LOCUS       DAORHF010000001        68146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_150_length_68146_cov_6.416105, whole genome shotgun sequence.
ACCESSION   DAORHF010000001 DAORHF010000000
VERSION     DAORHF010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 68146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68146
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150_length_68146_cov_6.416105"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="PKJ16_00005"
     CDS             complement(<1..1078)
                     /locus_tag="PKJ16_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45392.1"
                     /translation="MIRRIAIAVCIAGFIALARDSAIAQRDDGPAGPVETTVPAEPER
                     KKENAGEAPSGSAPFSHSKFVPDLSFVLDFSAVARDLEDRKFAALSIPEFMHGPAHAH
                     EGHGHSHAAMNEKRGFNLNYGELFLFAPVDPYFELTGVLHLSTTGVEIEEGYFATTCL
                     PYGFQFKGGKFFSGFGRINAQHAHQWDFSEQPLPYLAFFGAHGINEVGAQLTWLAPVG
                     FYLLVGGEALQGQNESSFGSEGFQTHAGGTPIVDATGETASVDIDGSNGPNLYTGFVK
                     TSFDIGDLTVMIGASAANGTTRINHELDEDNQEEGRHAVKAHTWVYGGELTLKYLIDS
                     YRYVSLQGEYLHRLMDGTRYGLVYD"
     gene            complement(1158..1427)
                     /locus_tag="PKJ16_00010"
     CDS             complement(1158..1427)
                     /locus_tag="PKJ16_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45393.1"
                     /translation="MIRTAALGTALVLMLSVVHVLLHHHHDGKAHSDCQVCVVMHLMT
                     STASIAVFAYAGSHVVKRRLVFVTYDCATAALQPPYYSHAPPIAS"
     gene            1757..2671
                     /locus_tag="PKJ16_00015"
     CDS             1757..2671
                     /locus_tag="PKJ16_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HNV45394.1"
                     /translation="MVRSYSIENFRITQCADDGGEILVYVDPTPEERALLVNDHLIDA
                     HTLQSSLDPDELARLEFEPEHAALIIKRPQNYSGGDQFLFRVSSYGIFLFKTRLIVVI
                     MEDIPLFDHKLFTRVYSLHDVVLKIIYHTIHHYLDHLKVINMISDSLEDKINTSMKNK
                     YLINLFMLEKSLVYYVNAINSNAFVIERLKNNATKIGFAPEGVEMLDDIIIENNQCFR
                     QAEIYSNILAGLMDARVSIVSNNLNILMKTLNIITIGIMVPTFVVSAFSMNVEIPFQK
                     HPLAFWMVMGLSLVAVLCFTLVWRFKKW"
     gene            2752..3324
                     /locus_tag="PKJ16_00020"
     CDS             2752..3324
                     /locus_tag="PKJ16_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HNV45395.1"
                     /translation="MDDMNTPTSDSIIQSHVIFGMTAGAIPLPLVDFVAISAIQIDLI
                     RELAKRHAVDFNANLGTSLVSSLAGTSLAKIGASAVKAIPGVGTALGIGAQVLLAGAT
                     TYALGRVFDSHFARGGTLANFNVDAMRRSYDEMVDTGKEFAQSLRKGASKKEDILATI
                     EKLAELRARGAISEDDFETTKKKLLDSLVA"
     gene            3321..3791
                     /locus_tag="PKJ16_00025"
     CDS             3321..3791
                     /locus_tag="PKJ16_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HNV45396.1"
                     /translation="MIVDHIAAARRYDRGTAWEDAFRFLATLDPAARENEYAIRGTDV
                     FARIFSYETLPREQARLEAHREYIDVQMILSGGERIGWYPRGGLSVSEPYDPSRDVEF
                     YAADREPSSWLALTPGLCAIFFPDDAHLTQVAGDTGPQKVKKVVVKIKRALAFP"
     gene            3844..5448
                     /locus_tag="PKJ16_00030"
     CDS             3844..5448
                     /locus_tag="PKJ16_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45397.1"
                     /translation="MSLRRRIMLLTCAGVLTIVFSSMGAAQQRARAFILYVPPMENQG
                     SRLHAWLGLAMADHVRDMLSTTDSIAVIGEDERLTALRQRAVDERAKDPDAALVRAGR
                     FLGASAVLVGRYTVRGSAVFIDRRLIDCRTGSTLTADSLAGTLPTISDLLERFATSAF
                     HSPGHGDGARTAPMTLTTADRASIAAYHRPPWTAFEHYARGRSLEAVKPDRAFTHFMK
                     AIQKDGFYTEAYIQAAALCYFKLDDPAQAFEYLRAALVISEQQHAPASMPSAGIAMRI
                     GQLYAAQGDLENALKHYEDSRQRYEKINRQASYPYIVLMGHIGEAHRLRNEYDRAIES
                     LGRAKEIAEQLDLADSEPYAELLARIGTLFYHRERYSRAEDLFARSLAIRTINETEQT
                     PAFVSLLNGLAGIYRQQNNPSKGLRYAEKGEALCRDPRLARTVECAALMSTTGILYYL
                     QQDYAKSVAAHEHGRRILDALALSDNARYAAALYHLGGSYIKTGDRSAAGRCFRTAHD
                     IYRTLGMQEQAEILRPFSEYYGKGTE"
     gene            complement(5454..6299)
                     /gene="nadC"
                     /locus_tag="PKJ16_00035"
     CDS             complement(5454..6299)
                     /gene="nadC"
                     /locus_tag="PKJ16_00035"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HNV45398.1"
                     /translation="MKRSIAERDIMPVIMLALREDIGDGDITSEAVFREPRVGAAEIV
                     AKADGVFCGGEVLRCVYRHVDEQVSVDILANDGDLVGPGDAVARLGGSVKSLLIGERT
                     ALNFLQRMSGIASATRRITSLLRGTGITVLDTRKTLPGFRLLDKYAVRTGGGGNHRFG
                     LFDAVLIKDNHIDAAGGITEAVRRVRDAYGDRFTVEVEARDPEQATEAAALGVDVIML
                     DNMDKEAMRIAIDGIAGRAKIEVSGNLDEDRIGRLVDLGIDYVSIGALTHSVKAFDLS
                     MRIVR"
     gene            complement(6296..8302)
                     /gene="uvrB"
                     /locus_tag="PKJ16_00040"
     CDS             complement(6296..8302)
                     /gene="uvrB"
                     /locus_tag="PKJ16_00040"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HNV45399.1"
                     /translation="MMPPFKVISPFRPSGDQQAAIERLTAGIRGTMKYQTLLGVTGSG
                     KTFTMAKVIEAVNKPVLVLTHNKTLAAQLYREFKEFFPENAVEYFVSYYDYYQPEAYV
                     AAQDLYIEKDSSINDEIDRLRLKATSSILDRDDVIVVSSVSCIYGLGSPENFEAMQIA
                     LHRGDDMGRDRLLRDLIAIHYQRNDVEFARGIFRARGDTVEVFPAYLKTAVRIEFFGD
                     EIEALYRIDPITGKIIDEIDSEYLYPAKHFVTDAEELSETVRLIQEELDARLRELTAQ
                     NRLVEAQRLESRTKYDMEMLQEMGYCSGIENYSRIIARRPPGSRPDCMIDYFKGNYLL
                     FVDESHVTIPQVRGMYLGDRSRKESLVEHGFRLPSALDNRPLFFEEFEAMIDQAVFVS
                     ATPADYELSVSEAVVEQVIRPTGILDPVIEVRPATGQVDDLIGQVRKRIDAGERVLVT
                     SLTKKMAEDLTAYLDEIGIRARYLHSEIETIERVEIIRDLRRGEFDCLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSARSLIQTAGRAARNVNGTVIMYADTITDSIRTTIETT
                     ERRRAIQRQYNEEHGITPATIAKEVGDIIEREYHPADEALSYVAEYREAYRDADPAAQ
                     LALLERIRADMIGAADNLEFEKAAVLRDQMLELEKRIDLRKKVK"
     gene            8471..8818
                     /locus_tag="PKJ16_00045"
     CDS             8471..8818
                     /locus_tag="PKJ16_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45400.1"
                     /translation="MLDISIDRKDRIAIMHIAGDFYIDSIASAERSWNEVVAAEPRII
                     ALDCRKLARIDSTAIGTLVKFFNMAMAKKIRLVFCDVNASIQLLFETARLKRHFVITS
                     AKKFNDKYLRQIA"
     gene            8835..9173
                     /locus_tag="PKJ16_00050"
     CDS             8835..9173
                     /locus_tag="PKJ16_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45401.1"
                     /translation="MFLVTIDHRGAVPILNLKGDLILDYVEHVDEVWNRVKEGQPQAI
                     ALNFKDLEQIDSAGLGSLIRFHYESKDKNIELMLLDPSPEILSLFEVAGIHILFTIVT
                     RAQFEADHPA"
     gene            9203..9730
                     /locus_tag="PKJ16_00055"
     CDS             9203..9730
                     /locus_tag="PKJ16_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45402.1"
                     /translation="MKGIIILTAIIACSCAINCSSSKKVGCVDGDCKSKNSAYYWKDG
                     SHYIGGYVAGVPSGKGTYVWADGKKYEGDMLKGQPHGRGTLVFPNGEKYEGDIQNNVF
                     VGKGTYTYKDGRVYTGEFKAGKPEGTGMMTWPDKTKYEGQFKNGTMEGKGVRTLPDGS
                     KEKGTFLPGGIFVND"
     gene            complement(9750..10256)
                     /locus_tag="PKJ16_00060"
     CDS             complement(9750..10256)
                     /locus_tag="PKJ16_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PCI domain-containing protein"
                     /protein_id="HNV45403.1"
                     /translation="MVRSLKLSLQKGFGFLTLFAGIFVLVVGVAMMFDDDPVHSETGR
                     GVAMFSTSILAPGAVLLYFFRRARRRLERLESIASVVKSYRRITVADLAGKIGVSIPE
                     ASELLAHAIAEGRIAGNFDRTTDEFFTEEARRQDVKFKFCPSCGAPLGRIFLEGETVK
                     CDRCGFLM"
     gene            complement(10281..14003)
                     /locus_tag="PKJ16_00065"
     CDS             complement(10281..14003)
                     /locus_tag="PKJ16_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HNV45404.1"
                     /translation="MIKDSKFREIPYNYTSFSDREIVLKYYDEETWGMLDQLRSHRVT
                     GRSARLLFEIYGDIFIIDRNPYLYDDYLYNPRKRKRLRRLHEMRLRAVEEGALGNQLV
                     LDLVKKTLVRGNEFFDELGGARRRRARIQSVLSHATSRANIHFTPFHRVSHATDASDW
                     RVEYPSVVVYPSRAREVPHIVRAARRLGLSIIPRGGGTGLTAGAVPVRKGTLVLNTER
                     LNRIEGIEFMDIAGSRVPVVNVEAGAVTEDVMEYCAERGYIFATDPTSAWASTIGGNI
                     AENAGGKKCVMWGTAIDNLLSFRIVTADGSLVEVARRDHAYRKIEPGDRVIFDVSVVK
                     RRGQRERLRTVELSGTDVRKKGLGKDITNKALGGLPGLQKEGGDGVIVSASFVLYKPF
                     HYCRTVCLEFYGNNMINASKAIVDIIEHFSENDRVYLTALEHFDDKYVRAINYKNKSK
                     RNEVPKAVLIIDIESNIEALLNAATVRTIALVREYNTEGFIASTPAHRENFWKDRKNL
                     GAIARHTNAFKLNEDIVIPIEQLPQFADFIDKLNLLKELSNSAQIVADVEAMLTAAGD
                     TDDADEFLRARIDEYRAHATAVRTTYLRYAENMNQTACDVLTDDPALSAERRSVFRLL
                     QDGVIRVDYECDVAEPFRRSFHNYDELMRAFDAAADTQRKRKIIIATHMHAGDGNVHV
                     NIPVHSNDYLMMAEADATVGVIMEEVVRLGGVVSGEHGIGLTKLRFVDQGILDGYAAY
                     KRENDPDDLFNPGKLNAAFPLSTIYTPSFNLLKLEAFILKATDLEGLSTSIASCVRCG
                     KCKGTCNTHHPGRTMFYNPRNKILGVGLIIEAVLYEAQMSKSLNFRHFAQLIEIANYC
                     TMCHRCAIPCPVKIDFGDITLNMREILTERRVSRVKTLTALSLFYLMRKGYYINKLFR
                     VLMFRIGYRMQNAAYYAVKPARRVIRAIVPTIAALVRTRLPAAGQKTFKEHIGVTRKN
                     AFIIIQNPTRSSGKSVFYFPGCGSERLFPDISIASQALLFAAGVRVVMPPEYLCCGYP
                     VLANGKSQSAAIKSYENRVIFHTIADVANYLEIDNVVVSCGTCFEMLQGYEVENIFNG
                     ARLIDINEFLAREGFFESAPRTDGVVYYHDPCHTPLKQLGYQKVISSLTGERVVNIPY
                     CCGEGGTLALSTPEISNQIRDRKRRCVGDAVLGKEVTILTTCPSCVQGLSRINGEVAV
                     TGMALPVHLAERFLGPDWKKDYLAAMNDRSIERVLY"
     gene            14251..15396
                     /locus_tag="PKJ16_00070"
     CDS             14251..15396
                     /locus_tag="PKJ16_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45405.1"
                     /translation="METAPTYLLIINHGRERGYETRELERDDFIVHTSRLLKRPRKVF
                     LGLGDFLEVGDYKKLIDSLFFNISGSAIETLLDDSNRQVSAEENIPFSFFKLAVYFPD
                     FILRDIPTSCASVIGKKGRLFPGAENFIKHIKPYDPLILTAIPHEIAIEFVKRLDLEE
                     RNLISTRYAVATNAKNREVYSGGVRVFMSGNRRSIEIEKVMNRENLSGEDIVYIGRGE
                     AGFNAFANMNSMAFNPTKSIIPESTITLYGSSLESLLVLFNFGGAITPLLTADAMEAN
                     LPSLVVFSEERSKRDELIELELSHRHLQNNIIGQRLEHSGESYVSVEREIDIEFGGSS
                     IDIQGVRDMVKNRMSAYRDHPQDFVHEVTNIARERYGRLYSSPEDES"
     gene            15393..16112
                     /gene="kdsB"
                     /locus_tag="PKJ16_00075"
     CDS             15393..16112
                     /gene="kdsB"
                     /locus_tag="PKJ16_00075"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HNV45406.1"
                     /translation="MITIGVIPARYGSSRFPGKPLAPIDGVILIERVYRQAQRANLAR
                     VLVATDDRRIFDAVTSFGGEAVMTGEHPSGTDRIAAAVRGIDADIVVNIQGDEPLIDP
                     RVIATVAAALENNAWADMSTAVTRLDAPEDIDNPNIVKCVFDQHGRALYFSRSRVPYN
                     RSATDHYHKHLGIYGYRKEFLERFISLPQSRLEAAESLEQLRALENGHGLHVSVVAYD
                     SIGVDVPEDVERVERILRAGR"
     gene            complement(16126..16902)
                     /locus_tag="PKJ16_00080"
     CDS             complement(16126..16902)
                     /locus_tag="PKJ16_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV45407.1"
                     /translation="MGDFTDGAQTFLCADRVNVRSAPSLKGSEVIANLPYGHAIRIVK
                     KSVERLSQNGFTANWYQIQFKEKGAQKTGYAWGGFFSYAHGVAKIDSTQVLFVSGLKH
                     RGSDGRYFAEIAALSGGRTISRIVYAPIYTDVSGTGEFGYSVYGTMHDGRKLSGVKGI
                     IELKFEYPACAYANGSMFFVFDGKRLIEACSAVTVSEAGVFHMGTVCLFPNQKGGRPD
                     RIVCTRKDEDFDEEKKEYVLKGTTVTVYQWDGTRFFEEKK"
     gene            complement(17024..18070)
                     /gene="ruvB"
                     /locus_tag="PKJ16_00085"
     CDS             complement(17024..18070)
                     /gene="ruvB"
                     /locus_tag="PKJ16_00085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HNV45408.1"
                     /translation="MDHTTRLMVPNKTDTDDDELSLRPRAFDEFIGQAKSVANLRVFI
                     ESARMRRQPLDHVILSGPPGLGKTTLAYIIANELGVAVKATSAPLLEKSDIASLLTSL
                     EENDVLFIDEIHRLAPAIEEILYPAMEDRTLDIVIGQGMGAKTIKINLAPFTIIGATT
                     RLGMLTAALRDRFGIPLRLNYYESADLQTIAMRSARILGRPIREDAAIEIASRSRRTP
                     RIVNRLLKRVADFATVQRKDAIDLAVTRYALEQLDVDGIGLDEMDRRILSLIIEKFNG
                     GPVGIKTIAAAVGEEIDTLEDFYEPFLVQIGFLNRTPRGRVATRSAYTHLGIPFAGAA
                     DRPESGGLFDSPEE"
     gene            complement(18076..18549)
                     /locus_tag="PKJ16_00090"
     CDS             complement(18076..18549)
                     /locus_tag="PKJ16_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV45409.1"
                     /translation="MIVSDHLNKDNVLTGVHARSRWELIDQLLQAAVKNRQVRGEDGE
                     EIKKALFEREKSMSTGIGKGVAIPHCSTDKVDDIVLVLAITDIGVNFNAVDNLPVRIT
                     VLLIVPKDKLTQHIKTLANIAKVLSNDALRQSLLELDSPESIVKAIRNFEKNAKK"
     gene            complement(18629..19687)
                     /gene="lpxK"
                     /locus_tag="PKJ16_00095"
     CDS             complement(18629..19687)
                     /gene="lpxK"
                     /locus_tag="PKJ16_00095"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HNV45410.1"
                     /translation="MKIFRALRVILAPVSLLYLLGFYVKRLCARPWSSPVPVVCIGNI
                     TTGGTGKTPVVIRTASALRENGFRPGVVSRGYRGRLSAAGAVVGDGERVLLSADAAGE
                     EAVLIAGALRGVPVVIGADRRNAISTIVDRFGVDCIVMDDGFQNTSVRSDVAVLVVDA
                     TDPFGGGLALPAGNLREPATALRRGDYIILNKCDLVRDERRERILARVRRISRGRPMT
                     TGRYRPRRLCRGADAGDTRPIESIAGSRVLLVSGIGNPAGFRALVGAQRPASIDEMAF
                     PDHHAYSKEDVDRIFSTSCGVDMVLTTEKDIIKLRRFPLPDALWYVPVDVVLEDERGF
                     VSFIADRVRAAAAKRKKL"
     gene            19868..20197
                     /locus_tag="PKJ16_00100"
     CDS             19868..20197
                     /locus_tag="PKJ16_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45411.1"
                     /translation="MIEIKDQGNGVFIIHIKMSEVHTLDVPELKEKLQKAIIDKGIKK
                     LVLDLSEVKMITSSGIGIFLNINQSLKSGLRLASPNAEVQKVLELTKVSSVIKIFDTV
                     DAAIKSF"
     gene            20356..20655
                     /locus_tag="PKJ16_00105"
     CDS             20356..20655
                     /locus_tag="PKJ16_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00251.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HNV45412.1"
                     /translation="MAFPPFIYIMQHNTAYIDITVKPRSSRRMIRVEDDSRIVAFINA
                     PPVDGKANEECIELFSDALGIAKSRISIFRGHHGRRKTLAIDGESAETVLRSLRP"
     gene            20652..21050
                     /locus_tag="PKJ16_00110"
     CDS             20652..21050
                     /locus_tag="PKJ16_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45413.1"
                     /translation="MIARIARIAICCLIAAVLATCGGRSWTVADRREGITKDQFRVCV
                     RVPRGSLSGIDPSGLSPALRRHADDRFRTLVGHLSASRRSIANDTARLDALMPGIVTA
                     GVIAHQSCEKDYCELFVDYPAKELRALVGE"
     gene            21047..21889
                     /locus_tag="PKJ16_00115"
     CDS             21047..21889
                     /locus_tag="PKJ16_00115"
                     /inference="COORDINATES: protein motif:HMM:NF017266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MnmC family methyltransferase"
                     /protein_id="HNV45414.1"
                     /translation="MTRTDRYIRIDTGDGTYSLLDRELDEAMHSRSGAYDEALRKHVL
                     PSRALEVPRATARVLDIGFGLGYNILALICERALRAPSLRLEIVTIERDAEIFPHLAD
                     VRFGDDRDAVYDNIRTLANRGIADGPGYTAILRLGDARQTIRHVDGAPFDAVFLDPYS
                     PAKNPELWTVDFFLLLRALVSDEAIITTYSSAPQVREALCEAGFRVGPGPSVGGKREG
                     TLATVSGPIPKLTAEALELLRDDPRSTPYRDPGLHSTREMIRAGRRDEMRSRRAVKKD
                     RQAP"
     gene            complement(21864..23675)
                     /locus_tag="PKJ16_00120"
     CDS             complement(21864..23675)
                     /locus_tag="PKJ16_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45415.1"
                     /translation="MSGKDFEKTKSNIGVKNLDDHTKKDLFNKFVQAGGQVVSEKKRR
                     GMTDFDRDKQRAYKTKIEEHKKKLGTRQPVGAVKSSPPSKKKPAASKGRDATGGGFFE
                     RLSIRFKLLFMGVTDFSILNLKPKFLNRINFEYRSALMEIQMVYLDVFKSNPVVSRAI
                     IEDLDSVKPVYFELIERVSSIYERSIVDRILNNYTESHTVPLRLDLVQEHVTALLRRL
                     VPIVAYQEQLQYAFDRAISMQMKLEKEKASVYSRKRKKAKNSVFVIYNRLYQELYWLF
                     CGIQGRVLPLQGGELEELLGVTDEERVGNRVRADQPKEIELPEAGARETEEENAEAEL
                     PDHIKKGVEMMFQLDLARLRKDYDRADIFREASDNDKVLVTYLLMQEFDKEYSIILTT
                     NKIKYNVVYSSGGKTDYRAVLSDTYNEMLKCLEGIRAYAESLATFAKAQRDKPTSNAQ
                     YIEYSKNLTSLEKEKIQAGRNAIASARMFMNKVSAELAEIVADMNGKQGIVDNPQDPL
                     AFNAEIEGNKKLDKKKIYEAITLVYQYTAGFAYRLGFEGDLSGNLEFSDSDRSVRAAI
                     ADKSAGTNRKDRADKAAPDAATEGENPSVIKELDDLF"
     gene            23866..24261
                     /locus_tag="PKJ16_00125"
     CDS             23866..24261
                     /locus_tag="PKJ16_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV45416.1"
                     /translation="MIHTLGITSRAREELIDITDAVADIVNAADVEEGVCHIYTPHTT
                     AGITINENADPSVRRDIITGLAHLDLERVPFTHGEGNSPAHVKSSLIGCAQFVLIERG
                     RLRLGTWQGIYFCEFDGPRTRQVLVRLSQ"
     gene            24344..27100
                     /locus_tag="PKJ16_00130"
     CDS             24344..27100
                     /locus_tag="PKJ16_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45417.1"
                     /translation="MKRTALKILRIALLWVPLSLIALVIVIFAALWLFLTPARVERLI
                     VSGFNERSHGTIALSVKEFDLVRGFRIDNLTITNGPEFDNTAFVRLERLVFRYHLLRM
                     LTGSVRFPEIGIYRPRIHLTQKNGVWNAARLMKPGEKKPEPEKPKEPETPSTADAITL
                     PISVDFLLKFVLDDLRLSVTSPAFSSNVEGITLNVDVWVPPFKKIPKSIDAVSLLERL
                     QIVLNPGEEINLSFYSKDAEVRPPLVCALRLAFDKKNGARPQFASVLRVGTNKTLVRF
                     KRAHLAPLNFMVSYDLFYQPLADLLTINHLEVAFLNKKWLRLTGTVNSVTAKQNINIR
                     MTESVISLDDLYPYYRSITGDHRTVFRGTISLFPLTVQGSIADLRVRGALSARTLVFR
                     NPAAEATLPSLTLAYTAHRRGVDMDITASLAMPHLFYTLNRNQSGDNGMTLTATVSAQ
                     NNFSRITIPGIAMRFFSPTLRRDVLALDLAGSVALKPALSGRISISRLAVDARPLPVM
                     VPRHLVKTVEGIPLKKPVTLGLDTDFSLGEAAIAARLALRGSVPDYDIPDLALGADIV
                     QHKASKRVTINRFSLGSARKNLSITAGGFIVLAEPPFSDADLRLTLSYRPPSFTTAYG
                     PWKLKGSARVDAAVKGGFKKGKATGAITVDKFFVKNDESLLAVDDININFPFEYYFTP
                     RYTGQSRITVDKAQIIENEFFQEKQNVTVASVRAKHPARNVPMEYLKDLRATMSFRNN
                     TFEIARLRAYVLDGALYGRNILFNLADMKKQNMEFNLVLDVTNVDIGKLDEQEAAKKK
                     RDAELSLNANFKGHGLDISRELSMEGFINIHKIGDKFANRLLKGLSTEKGQSKLGAIG
                     QFAVDNSMNVTGFRFNLDKGLVYATVTFSRRTFGYLLGVKNERVEFERMPIQEYLRKV
                     QTGD"
     gene            27102..27707
                     /locus_tag="PKJ16_00135"
     CDS             27102..27707
                     /locus_tag="PKJ16_00135"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HNV45418.1"
                     /translation="MKRALAATIFVIIIASIAATGCSSSQIPSCLEISPPDIHMTGEK
                     TVIEKQIVGDYKELEKDAWIISSARTNVQRTKGAGPAVTGDEELFRAMKIREFHQSKL
                     RTYKNEGAIGEANTGLVAYLNAAPYESDRDLKRLLMIFIEEENKARRAIFRRSLVKSG
                     VATPDDAALAGFGRAFAEEQRALAKPNDWIQDARGAWTKKK"
     gene            27718..35694
                     /locus_tag="PKJ16_00140"
     CDS             27718..35694
                     /locus_tag="PKJ16_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45419.1"
                     /translation="MNGFTLPRGIIVVLAAFVAVSPLACGKATREVPFKYMDIIKTEY
                     KYNVGKAIPVTIDQSIEIDGTFTGDGAYFFFSSNRESGNYDIYLRNLADITTVRIIRH
                     PAKDTAPAISPDGRRLAFVSQREDPEGDIFVAPIDPAAIIAAAKSSVDEIPPLDGASR
                     NLTQFQDPQTGTIKIVRDASPAWSPDGTRIAFSSTRDGAENIWIMDASGANLRRITTK
                     GGLYPSFSRDGKRIVFISYRDAGSRGNIYTVDVDSGTERQITKSSAIKLHPCFVANDR
                     EVIYTLIDRDTNGDGRIDLADNAVIAYQNLDTGAAYPLTLHSHSSFMPRWFPVFIDFE
                     KSYRGVVLYSEQIGQNININVIPETGIIPRSANAEEQFNLALKYLREFDDYERYELAL
                     ERVFHFFGSGKDARSVIFVTKALIEEARYALSRNNARKSRELAARLTSLSSDALDYRR
                     IAGAYLSRLNERRPGEETLRGAIARLSARDPKSEFIPYVMEDLAAELMRVGNMAAAES
                     TLRAIAAEHPKYRRAAAINYTLGSILFQSALDPLPQPLIQVLDTGSSFQKIDTALLLI
                     KRYQAVRDPQRRIDAARRAIETHPGKKSLAGTMLYVIGLSRIEQGKLAEARTALSEAL
                     AQVSKFDLIFFRANVALGGIAERQGNANDAERFYSAAANNYLLSWNQPDIRTVIRKLI
                     DHYEEHGERLERAGSWNAAVDLYKKYVWLVTYIHLNKRFEDLYNEYAPRAHVLYIDAH
                     AAWKGNSAESLSALEKEYLARLPIARNDFDKAHIYGLGYIYARSAITNDARASSVLPG
                     IASITGTRLPDLLAGLKRAVDQIEWALFIDDTFVDAYILNGWIFQYVDLLRREDAATA
                     GGKNERVFARFFPKYLWERSIPLYEKALTTNDETANPEAEGNLHLNIANCYFLLNNYP
                     KALTHYALTNRFKNRFNSPVEEALFRFHYAYCLWQNGDIANAREEMNSTLSIYRRLAS
                     GKNQRAYKHQILYLYRYFALFDRMEGKHASAIDWYQRIIDFSAENGIEIDRARYLQEI
                     GHCYGELGDTDRAQRFLEQSQVILVDAPDRRQTHKIKWRVFGISFLVGQFEFFDLGPD
                     VAVIGDNRLYSELDTYNKKLLNLSLFESYATGRADHAAAIGRLTEKLALLKKRDYRVN
                     REAIVRTLNNIGYHHFMLRDYAASAEHFRAAWRSAADPKINDLDGMFRAVMNMVNLYA
                     YRIENGRIADSAARTELDELARDIASYRAAYETGRLETGLKQLEAAAKARREAVTERQ
                     RDALREKISTDAAAIYFSLDIAQGIVTFYRAELRYRAEFAGKEPPAAFDIYRHNRELY
                     DLYADARSAFDAAVANTSRVRSREFAVKLLLNRAICQTRLAQYDEAADSYREAETIAE
                     KFRLRSILWEARFQAAVFLARHGQQTGERNHIARAEDYFTRAAEQLERPPYRASANLD
                     RVRAGIIAHARFLARRGLHERAFAATERLARIVRAHRFAASSLSFPSPKDAAAVSAYT
                     KLHASIESDEHALTAFLEGGGDIKADRARALASSISEHERALADLRSRLDRANPHLSS
                     CLFVQPVPVRAPAGSVVYAFLDNDDTLIAWTLAGAALRHAQVPLPQGQSSISDAVRSH
                     ITGDTANAHSRRFIILSDTSLAAYAGRTAPEMPAAMLIPALDRVSHALAADNVPISRG
                     LVIGVDAARVIKGRTAFSALLFTALDSAAGDLPHSQLIFDDTADGLSPASIISRGLRP
                     RVLIKRLRSTNYTDDLLFFSEAALYAGVRSLVVYTGLSTAEAADLVEATLSRPASEIA
                     STAARRGAILAFGNPDKPGQAGADPQKSATDAFSRYTRFLESRRFDDAARYLTIWRDT
                     AARDAATNARFHLLMADLAYRRGDLAAFGASIGQARSLTPINTSDHARALGGAFLQEC
                     LSGNAVTARTIFDEYRARTNRPAGDGMEIMQALCALMEHAPERSLAAVRGLSAARITE
                     PHAVRLLPVLLRFAELYGHRQLRASLIATMRSEPLRSALASSISDIDAIRMAAIPDAD
                     GIVPLPDDVRWLSSATDAATLRARAFEATAATTAYTVRTAPLLSASIRALLARYRFDE
                     VSALIGRIRTGDMIAASHWMDALALLDTIRAIHTAYERRDEAIATVTASTTLLERIPI
                     ASLLADRLYDEAVAHLARSDHARASASVNRALAITPDGADLKTRLRLLEIELMITQGS
                     VDAANTRLAAIESTLPDDYRYDRSMLRSLIVRHGIIQAAGQRQSAVTELLQTYDRLIA
                     DAMDALDATPGRVRTVRRLALIEQGLDFSISYRLSARRLVDSLSLFEAKTQISFRARM
                     SGELDSRAVPARARGSFRALTALQAGDPEDVRALLTEYPQLMVLSSVRHVPIAAFQAS
                     LPADAVAIVVARSEADLYIWTLDRSDINAFRIRNGFVRMTKLIADYHELIRERRHPAV
                     LSTALAAVFEPVEKHWRGRRTILFITDRNTEAIPYELIGRESILDETHAIAFAPSILA
                     ALREYPSVRPTVLLGSAGAPSLAANLDRIAIRESGITVDSGSRSTGFTHVEGLVAYHP
                     LAGAVYVDSVPYRRALNASPGAYLASLSPLERQAGNDLALLGAIGGSSVTVITAAEAR
                     DVNAALFTDAFYREFSRSRDLVRASSRGRAALRADERYRHPAYWCGIRLYLSSNRHLS
                     RGVK"
     gene            35749..36957
                     /locus_tag="PKJ16_00145"
     CDS             35749..36957
                     /locus_tag="PKJ16_00145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV45420.1"
                     /translation="MSDTRHPQRTIAIVDDDDAVNQVLTRILQREYAVRNFLCAEDMI
                     AENAAASVDAVITDVDLPGISGIEFLSKLCADDPNLPVIVITGYNDIDIAISALKSGA
                     FDFILKPFNNDQIILSVRRAIERRRLLMENAMLIETLTAKNAELEELNARIRARNVEI
                     ENELDIASNLQRCLFPASLPAIPGLEMALQYHPVEKVSGDFFEFDHTDDRRFSLLLAD
                     VSGHGLPAALYSAMIKAGTDTINTRSALPSEFMRAMNTFLIRTQKKMSYSFATLFFGR
                     FDLADNTITYTNGGIPAPILIKRGGEVMALDPNASFIGIFDSSRYRDDVVPFEPGDTL
                     LLYTDGAFEIQNPEHDILGHNTFIEIVLSHRDRPITEIVDNTFNDIRSFFPNGSIIDD
                     ITILGMRRTG"
     gene            37031..38095
                     /locus_tag="PKJ16_00150"
     CDS             37031..38095
                     /locus_tag="PKJ16_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV45421.1"
                     /translation="MKSIRHAMPVRMCAAALIAVSLFAPATLHALEITFMVGSVSLTR
                     GGKTSAPAVRTRLASGDTLTTGKGSLADLALADGSQIKVMENSSIRFSGDIKDRPDSV
                     SVIAGIVESKFKKLAKGSQSRIITPTTVCSVRGTEFRVAVSDSGDSRIDMQEGAVELR
                     NPYGKTAIRNRQQSSVRMNDKPAAAREGAGLEQWKEQRDRALDENPREQGGRIKTYLG
                     TMNDRSAKTSDRISGLGSDLRDNAPRTKRALKKSGTELDRLDEEVKDDLYLSEATNES
                     LGRMSERFSKGQGDIRELFTRLKEESDRVCEQKRKNYESIQAVKAAYRKAYDEIMGTM
                     NTEREKIRRQFDEQRETKPE"
     gene            38076..38561
                     /locus_tag="PKJ16_00155"
     CDS             38076..38561
                     /locus_tag="PKJ16_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV45422.1"
                     /translation="MKPNPSEASVMRVYLVQHADALPADADPARPLSPTGVRQADGAA
                     RFLASLSVRPDRIVHSGKERARHTAEIIATALGCASVEPRAGIDPNDDPAPIAEELIA
                     ATDSPMIVGHMPFFRRLANALLGTGALDPIDFDNASPLVLIRDGGRFRIEAYAKNAII
                     A"
     gene            38660..39694
                     /locus_tag="PKJ16_00160"
     CDS             38660..39694
                     /locus_tag="PKJ16_00160"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV45423.1"
                     /translation="MKTRITELFKIKYPILLSGMSWISTPELVAAVANAGGLGILATG
                     ILQPDETRDYIKRTRELTNKPFAANVTLYFPGADRNAEILIENKVPVVNYALGKGDKI
                     AKAVHAYGGKVIATVTTLKHAMAAQRDGADALIVTGHEAAGHGGAVTSLVLIPSIVDA
                     VDIPVIAAGGFADGRGLAAALLLGAEGISMGTRFMNTTESPVHESQKALSNTLTVYDT
                     VYTDKVDGLPARVMASKGATRLINKRLNPLSALINSRKIAKLLGFPWLKLAVGIMLSG
                     VQNSIRMARMAIGFDAFKAGTMDGDNENGVLPIGQVTGIMHDTPSVKTVMDRIVKEAA
                     EAGKKLGKKL"
     gene            39711..41102
                     /locus_tag="PKJ16_00165"
     CDS             39711..41102
                     /locus_tag="PKJ16_00165"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV45424.1"
                     /translation="MRPALEELAPRIRTALATAGVEYAEVRISEGSMTEITLSGEQVD
                     SLSTGDFAGGSVRVRSGGGWGFASFNSIGDIESFIRRAAAIVLPSVGGAAPGIRRTPA
                     ISDRVAIQSRIPFTDVPLDAKYELIAGYNAILRGSASIQTTRATYRDVAARSLFINTD
                     GSELYYDRSYCGIALAAIARDGSNIQTAGESVSGYGGYEIAQQRESIAERAVKTAVDL
                     LGAERVGGGTYDVITDQKLSGVFIHEAFGHLSEADFIHENPRMREIMTLGTRFGVPEL
                     NVIDDGTVPSLSGYVPYDDEGVPSRKTHLIRDGILAGRLHSRETAAAMDEELTGNARA
                     ISPMAQPIVRMTNTYIDNGPAETGELFDAVGNGIYACDVIGGQTNLEMFTFSAGHGYE
                     IKNGKRGKMLRDIVLSGNVFTTLNAIAMIANDRAMFGGLGGCGKQGQGPLPVSFGGPH
                     MLMKNILIGGVQR"
     gene            41099..42397
                     /locus_tag="PKJ16_00170"
     CDS             41099..42397
                     /locus_tag="PKJ16_00170"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HNV45425.1"
                     /translation="MIEAIDKRLRGACRWYDCLCTQSESTVVSFRDNRLHAVRESQNS
                     GCGLRVNIDNRVGFSFTNDASTPDGLVDRARATAPYGDIDDYDLPGRATLPATEPYRP
                     SIDSFSVEREIDAARALIAELRGRFPDLSVDLTASRATGTRRLANSTGFDASYRHSSY
                     GISIHANYVFDSGARLLVWEGTSELGPVDYRHLLDRLITKLDNAMTVRTAPSGKLPVL
                     ASPRAFASLLGILKSGLSAKAVYKGVSPFQNKLGERVFHEAFSLRDNPLAEDSPAGYP
                     FDDEGTAAADKIIIDRGVIAGFVSDLKHAARLGHAPTGNASRGYASLPVPSFSAVNVP
                     GGVTASVGLIEGMDRGVLVEQFIGLGQSNTLTGDFSANLDLAYLVEGGRIVGRVKDCM
                     ISDNLFSLLAAPFELSRDIERVGSATLPFAFFPAVNYTAN"
     gene            complement(42403..43464)
                     /locus_tag="PKJ16_00175"
     CDS             complement(42403..43464)
                     /locus_tag="PKJ16_00175"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HNV45426.1"
                     /translation="MTGKKILTWIVIMLVIAVIFTVKLLHQAGVFKTIEPMKNAPYAC
                     SRVTDIPGPEDIVIDRAAGIAFISSDDRRAALRGKRVPGAVYRYRLTVEHAVPENITP
                     ALAFEFHPLGIDLYRSGETVRLFAVNIRADGKYIEIFDYRDGALTHVESLHSDLITSP
                     NDIAAAGPRSFYVTNDCGLTSPLGRTVENYLKLPLGSVVHFDGARFTTAARRLVFANG
                     IALSPDRKRLFVASMLGRSLEVYDRDPAQGTLDHSASIDLGTCPDNISVDDTGALWIA
                     AHPQVMKVLPHRDNPSLLSPSQVLTVRFGPDGDFRITDVYVDPGTGISASSVGAATDG
                     RLLIGTIFDPMFLDCRARK"
     gene            43633..45015
                     /locus_tag="PKJ16_00180"
     CDS             43633..45015
                     /locus_tag="PKJ16_00180"
                     /inference="COORDINATES: protein motif:HMM:NF013836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HNV45427.1"
                     /translation="MITYAGFDELIARVYAHRQDHVFEYWGELIDAERRELLNDLTGI
                     DFEGLQSLFAQTRRSDAVAGEFGPAPFIPIPKTDGENAQRAQAREAGIAHIRARKVAA
                     FLVAGGQGTRLGYDGPKGKFPVSPVAGKTLFQIHAEKVLASSRAYGVDIPWLIMTSIA
                     NHEETETFLEEHDYFGIRETLVHVFPQNLIPSLDANGSLILESKNRIFKNPDGHGGSL
                     TALKTSGALDFLAEHGIDTLSYFQVDNPLVRVIDPDFIGYHELNTADVSSKAVTKAGP
                     EEKVGVFVRFPNGACGVVEYSDMTHEQQHLQDCTGALVYSMGNIAIHLFSVEYIRRLT
                     AGGAIELPYHVARKKIKSLRAGAPAEIDGFKFEKFVFDAIPLAERSIVFETGRAEEFA
                     PVKNRTGVDSVDTAREMMSALFRSWLAARGVPVPPGVRAIEISPLLALGPDDLPADLT
                     VPDRPDVYLS"
     gene            45012..46916
                     /locus_tag="PKJ16_00185"
     CDS             45012..46916
                     /locus_tag="PKJ16_00185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HNV45428.1"
                     /translation="MTRSVLFIQLPLLDHGRFYIAGNVDYAPASLTAYLHRRFRNTVR
                     CESLPDTLANFASNRLIARYIDAVDPEIVAFTCYLWNIERNLEIAREIRERAPRRTIL
                     FGGPEIAQGSWALAEPRDAVDAFVTGEGEWFFSRLLGGVDLGPYRTDIRGNAVYAQPA
                     AELLSADEIVEPFTAGYLNTMNDGSIFLELVRGCPYRCAYCNYSKNSATVRELPFDRL
                     VTAIERGRAHELTEIYILAPTFNRSSDFDPLLRRLAAMRHGVRLHTEMRTDGIDTDMA
                     ARMYDAGFRSLEVGLQTLSTRALREIGRSSRTDAELAGMTALRDAGIDLKIGIIPGLP
                     GDTPEGFIGTVTRLIEEGFGDQIELYPLLVLPGTRMRDIASNRSARFQSAPPYYFTDG
                     WGFDEGMIRDIVARVRAETGYEYDTIRIPDCLAGAGSGIVGGVHFRGDDPDRWDGARY
                     RDIIDTNVFTITVSLGSPDAIVPGLPRLFSLLPADVLYTLIFVCDLPIPEQPIVDFLT
                     RRESDSFLMRLRIFDEPKRDSRIQCFQVITDPAVYRIAHDTYRVIEPIFRLTMDTMDA
                     IPLVRRCERLGMVLAPGVYEHVARFIITEFSDRPDRIGFESETDMARFYRECGLDLVR
                     VPLTPSVLDR"
     gene            46942..47670
                     /locus_tag="PKJ16_00190"
     CDS             46942..47670
                     /locus_tag="PKJ16_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV45429.1"
                     /translation="MAFAISIRLIVFLQSLIVWIVRELSRVFAVSSGSGRRSRAADGP
                     SDEELVRAFTNDTGRPQERNRAFDALVRRHQHLVFSLCARILGDYDEASDCAQEVFIK
                     VHHNLGAFKMEASLTTWLYRIAVNTCRNRMASAQYRARQRSVNLDAGDDDSADGPPRE
                     LGDDSFAPENLVMRREDARAIQRAINTLPKAQRLLVVLRDVEGKSYSDISAITGIKEG
                     TVKSGLARARERLREILREAFDDV"
     gene            48152..48718
                     /locus_tag="PKJ16_00195"
     CDS             48152..48718
                     /locus_tag="PKJ16_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45430.1"
                     /translation="MRSKSDSPRFGRKQKTAKPGIVADRPIEILLAIADTRSMPADDE
                     IGAGDMATMREAEEKAPEAPAALKSESRDTKMALSRKRAADGMARGSSIERSKDAPAR
                     PALRDSGTAIRSITSDVRGRVIAEERDPDTRMLRAITIDVPAAKYGTFVERLAGVGRI
                     SMPPKREGPHGRDWVRIRIRTQRAPHSR"
     gene            complement(48715..49230)
                     /locus_tag="PKJ16_00200"
     CDS             complement(48715..49230)
                     /locus_tag="PKJ16_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45431.1"
                     /translation="MLQREHGIPYQMPLAVKSLRCSMQRSDIKTMKGYMIRHAGVVVA
                     CLIAVSALACATAGEKSGVISHDGKLAPLTFITIEPVYARTKDDPSKSVFIELSLGVR
                     NRPESAAEIRSRVPEIHETVKSAIRVRHHFELYSVNDYERLHVILKQDINRLLRKATV
                     EKVMFLNFIVR"
     gene            complement(49544..50260)
                     /gene="rsmG"
                     /locus_tag="PKJ16_00205"
     CDS             complement(49544..50260)
                     /gene="rsmG"
                     /locus_tag="PKJ16_00205"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HNV45432.1"
                     /translation="MEHEFRRFIEESGLAAMVDDRKLEKYSAFTALVVEASTRFNLTG
                     HKTPGDVFVDLVIGSIAPLARLNVPRRTFVDIGTGAGVPGIPLAIQYPDWTGTLIDSN
                     AKKAAFVRDVIQQLELPNLTAICGRVEEIAHDRAHRGRYDWVLSRAFNALYVTLELGA
                     ALAGPQGRCYVYSRETVGSLPQEILAHAHALGLVPSTSDDYEHVLGCSDGLLFRKAAR
                     TPDRYPRRYTIIKRDAQRIG"
     gene            complement(50250..52184)
                     /gene="mnmG"
                     /locus_tag="PKJ16_00210"
     CDS             complement(50250..52184)
                     /gene="mnmG"
                     /locus_tag="PKJ16_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010944073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HNV45433.1"
                     /translation="MIRTYDIIVVGAGHAGAEAALAAARMGMNTLMLTGNLDTICQMS
                     CNPAIGGLAKGHLVREIDALGGEMARAIDETGIHYKMLNRSKGPAVWAPRAQADKKAY
                     QLRMKLALERQQNLSVIQDIADRIIAESGRVTGIVTVRGQEHRARAVILCTGTFLKGL
                     IHIGEYNERAGRLADFSSEGISDSLRELGFPVMRLKTGTPPRVNGDSIDFSRCEPQYP
                     DERPTPFSYATTTIEREQVPCWITYTDERTHAFIRNNIHRSPLYGGKIKGIGPRYCPS
                     IEDKVMRFADKIRHQLFLEPEGINTNEYYINGFSSSLPEDVQLEMIRTIPGLEEVRVM
                     RPAYAVEYDFVPPSEIRPSLETKRIAGLYHAGQINGTSGYEEAAAQGLMAAINAVRQI
                     RGSEPIVLRRSEAYIGVLVDDLVTKEITEPYRLFTSRAEHRLLLRQDNADSRLMRYGY
                     ENGLVDEARYEAMREKYARIQSRIEDFARRTVLVDEKAAQALAGRGKAINPCGRIPIE
                     KLLKRPEIGIDDVLSLIGESMDPELAPIVEMEIKYAGYIQKDRELIARLNRMEERIIP
                     DDVDYRAIAGLKNEAREKLVRVQPRTIGQAMRISGVDPSDASILLVHLEARERAAKKE
                     KRKENADNARTNRGDVPRGT"
     gene            52491..53027
                     /locus_tag="PKJ16_00215"
     CDS             52491..53027
                     /locus_tag="PKJ16_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HNV45434.1"
                     /translation="MNDRSKYISRLHVKKGDITKESVDAIVNAANPSLLGGGGVDGAI
                     HRAAGPELLEACRTLGGCRHGEARITKGYNLKAPFVIHTPGPIYHGGSSGEAQVLRNS
                     YENSMRLAHEHALSSIAFPAISTGVYGYPREEACRIAVDAVLDFMATHDYHIDIYFIQ
                     YDNENYTIYKNYIDGLGK"
     gene            53024..53866
                     /gene="pyrF"
                     /locus_tag="PKJ16_00220"
     CDS             53024..53866
                     /gene="pyrF"
                     /locus_tag="PKJ16_00220"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HNV45435.1"
                     /translation="MNYLELLKETAKRFNSIVCLGMDPVIEDIPITTGSVKERIVAFY
                     AEMLDAIEKRKVYPAAVKPNYAFYAQYGLEGIEALVSVCERSRAVGLPVILDVKRGDI
                     GKTAEAYARESFEFFKADAVTLSPFLGYDSIEPFMKRFPGKGYYVLNKTSNKSSAELQ
                     DVRVGEDTVYALVSKKIIDWYQPGIGAVVGATYPSQLSEILGIYAHAGKEVPLLIPGV
                     GSQGGDVAEVIAALKKSPSVRIHRINSSSAINFAYKKHPGMHFAEAAVNELERLNAEI
                     NGLL"
     gene            complement(53881..54663)
                     /locus_tag="PKJ16_00225"
     CDS             complement(53881..54663)
                     /locus_tag="PKJ16_00225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HNV45436.1"
                     /translation="MLSHIDAFARGIIRDAGAVLLRGFRAPDLVVEYKSRTDLVTNID
                     RESERLIFDALRREFPGHVIIAEEGSRAEEGDDYVWYVDPLDGTNNFAHGIACFCVSI
                     GVYSRREGRMAAGFVYDPFHDELFHALHGSGSFLNGAPIRVSTTDAVDISIVATGFPY
                     NKKETENNNSREFCSVMPEVQGVRRFGSAALDLCSVACGRIDGYWERQLKPWDCAAGA
                     IIAEEAGGRVTRYDGSNFDPEYPEVLATNGRIHERMMALLRV"
     gene            complement(54666..55718)
                     /locus_tag="PKJ16_00230"
     CDS             complement(54666..55718)
                     /locus_tag="PKJ16_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45437.1"
                     /translation="MWGTLIGLTLAMSALGAPLHAERTPTLNEKGIELGNQKKYDQAI
                     ELFEQSLQGQNAASAIVMHNLGWVYEMKGDVKNAIRSYRGATERNPRQVASWERLGFL
                     LYKTGDYLASVRAGEAALKIEPDNRNVIGWLTEARKQIRTMYEEGTVGRFVQEKEAQE
                     EQTRPGFLSRDIFLKAGYVPIYEVMSDELERKSFAMRGFATMLETNIFLKWVWVGIDF
                     EWQRTVISDEIGYYQYDFLNPGISIKHVLDRGFFMGIGFSGKSLIAVNNPSANPVKAR
                     LASDLWGYIIMGYYLPISTSFALMFENRFGCNLTNNTFREYTGTAEHVAIGFSYDMTF
                     YLGIGWRFYTADQRKD"
     gene            55868..58120
                     /locus_tag="PKJ16_00235"
     CDS             55868..58120
                     /locus_tag="PKJ16_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HNV45438.1"
                     /translation="MKKTIMIIGGGLLQDPVIQTAKKMGLQVIVTDYNPNAIGMRHAD
                     IPIVMSTRDIEGSVRVAKAQNNITPIHGVLTVGTDASMTVAAVANALNLPGIKFEDAE
                     AATNKIKMRKRFHEHGVLCPRFYPVWSIADAKKACNKLGFPVVIKPSDNMGARGVTRI
                     GTKNEIADAFQFAKSASPSGELLIEEYMEGPELSIDAVVFNGEITITGVADRIIEYPP
                     YFVETGHTMPSHLPAEIQENACEAMRKGIRALGITHGFAKGDIKITKCGPKIGELAAR
                     LSGGFMSAYTYPLATGVDLMRAAVEIALGQEPGNLDPVMNRVSIERAIITTPGIVKSI
                     TGLEEALQIPGIEEIFMNVQPGDKIVIPRSNVEKAGHIIAVGNTLAEAEAAVARCREV
                     LSIELFEATELSQETINIAAREKLKKTCYVCKNCDGKDCPSGVPGMGGAGTGASFRRN
                     VESLRNYKINTRLIHGVTAPDTSTSFFGINLSFPVMAAPITGTMTNMGGAMDELDYNR
                     AVIDGCLQAGTIAFVGDGATPNKYKIGLQAIGEADGMGVPIFKPRSDNRELIKRLRAA
                     EKVHSVAMGMDIDAVVFKTMALKNQPVGPKSFDDLKALISMTEYPFILKGIMNVRDAV
                     MAVEVGAHGIVISNHGGRVLDEMVGSMDVLEEIAAAVKGHIKIIIDGGFRTGVDILKA
                     LALGADAVLIGRPIAIAAVGMGAAAVSYYLNGLRRDLEQAMILTGCNGISDITSDVVR
                     KINGKTLTYV"
     gene            58181..59089
                     /locus_tag="PKJ16_00240"
                     /pseudo
     CDS             58181..59089
                     /locus_tag="PKJ16_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(59297..59800)
                     /locus_tag="PKJ16_00245"
     CDS             complement(59297..59800)
                     /locus_tag="PKJ16_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45439.1"
                     /translation="MKTALCDASSLIILYKAGLVRAFVVRYRVVVPASVFEELVIKGK
                     TGAERFRAYCAEGSITVEDGLSFADVQIPLRGGEAECVRLYRAGRGDFILTDDGKAAD
                     YCRASGIPFINALLVPKLLLHAGILTEVTCADAMQRVRRFGRYARWVVDYAERAGLDE
                     LCAFMTE"
     gene            59990..61363
                     /locus_tag="PKJ16_00250"
     CDS             59990..61363
                     /locus_tag="PKJ16_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV45440.1"
                     /translation="MKTKAPLKPKKRNRSSAASKRLPHWVHLIAQDGYDTFAIAAKIK
                     AVMDRESVSVAGKSVFLKVSFVFPVRDPERVKHIITNPAFIAGVAQALVERGASVVYI
                     GDAETFATARYSFDIVDMQKALKALPESIRKNVVCCYLDEVYKEWITPDDPFCPEIRL
                     DYPKIVRDVDVYISLPKLKVNIFADITLSVKNGMGLITKETRLRYHNENLHAMIADIF
                     QVRPPDYVITDAVFAGEAQGPMEATPYPMNLILFGNNGPSVDTVCCNLMGYDPREVKH
                     LMLLHERGFGPIDLKTVRVENAKLLNERRHVFTRPSMELAHLSSNITCHQGECCASGC
                     LPFVRALLDGYGLNRGWESLGEYTIIMGKNLDIPEATLKKLKKRKTLVYGDCVERYRK
                     YGVFYRGCPPDYINALITFPIRTSLGRTPWLGYVEYWTRIKTDLLHLLSKLRGKKYRT
                     LRGRVKK"
     gene            61460..62332
                     /locus_tag="PKJ16_00255"
     CDS             61460..62332
                     /locus_tag="PKJ16_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV45441.1"
                     /translation="MFFIKYFTGQPSEYIIKFGRGRIRKEGQGISFFYWAKRNTIVSI
                     PATTRDVDFIFNEVTKTFQAVSIQGQITYRIQDKGKIFANLDFSINPVTRAYRSEDPD
                     KLAQRIINAVQMLTREQIQGLALEDALGSSQAMAHGVMEKLKTDARINAMGVSVESIY
                     FTAVTPIPEIAKALEAEYREMLQKKADEAIYSRRASAVEQERIIKQNELNSEIELEKR
                     RQELVEQSGANRIKEAEYKSKAMELEWSPYRNLDPKLLVALGMKSLGESGAKIGNLNL
                     NPDLITLLLGLDKK"
     gene            62329..63345
                     /locus_tag="PKJ16_00260"
     CDS             62329..63345
                     /locus_tag="PKJ16_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HNV45442.1"
                     /translation="MTSAFDKIVIVTKETQMDQLVRAFATKSQAKFYLAQAAQAAAPV
                     NAQSTQKKAGSAKNIQAHQVKSEFDEISLADSAYKEALKNLQSAIPAGINVQHIDWSY
                     LPTFLFGEKDLVIALRQDGLVINIAKYLSEQPIVAVNPDPARYDGVLLPFTVGDFNHH
                     FRRIVSGEFGVSAITIAKASLNDGQTIHGVNDIFIGPRTHTSFRCNVSFMGREEYQSS
                     SGIIVSTGCGSTGWFRSIVEGARRISSGFDNAKPAKEQSRFPWDANHLFFSVREPFES
                     KTSKTDITFGKITTGDPLVVTSLMGRNGIIFSDGTESDFIEFNSGKIATITLSDRKAK
                     LIKK"
     gene            63490..63774
                     /locus_tag="PKJ16_00265"
     CDS             63490..63774
                     /locus_tag="PKJ16_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45443.1"
                     /translation="MMETSSHRSPARSSALTIAIATVAILLPLSGAALFSSVDRGRLC
                     EDRCTQKFWTCRERAFNYEGKRGDRAIAICDTMFTNCMGRCRLYSQPKRR"
     gene            complement(63793..64491)
                     /locus_tag="PKJ16_00270"
     CDS             complement(63793..64491)
                     /locus_tag="PKJ16_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HNV45444.1"
                     /translation="MNFKGIHKTSLIDYPGKICSILFTGGCNLRCRYCYNPDLACNNC
                     ALETLTNEETFSFVERRRHFIDGVSISGGEPTLAKNLDSFIKSVKDISLSVKLDTNGS
                     NPEVIKRLIEKGLLDYVAIDVKTSPSKYDFLTGTTFRFADIVKTISIVRDAGIDYEIR
                     TTCIPFFVTLDDFVAIKREIGRVKQYYLQQFVNSKTLDDDVANYQPYPAEELHRFREF
                     VLSFADLCEIRGLS"
     gene            complement(64454..66742)
                     /locus_tag="PKJ16_00275"
                     /pseudo
     CDS             complement(64454..66742)
                     /locus_tag="PKJ16_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
     gene            67127..67648
                     /locus_tag="PKJ16_00280"
     CDS             67127..67648
                     /locus_tag="PKJ16_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45445.1"
                     /translation="MKYAIFGTLALMSIFLLFANSSAEVGADIIDKIKLTDADIPADF
                     AYGVLPDRVKRVLKGNPAQLDRAAIRKVIKDMELYPGGDHTTVLAMHAAVIAHKKNPH
                     DDDIVCYIIVYKDMKSAKHEIEKLSALVGANKTRTVMVTRDNCAVFLFVDEEANFHHI
                     QKMAGTITARLKK"
     gene            complement(67649..>68146)
                     /locus_tag="PKJ16_00285"
     CDS             complement(67649..>68146)
                     /locus_tag="PKJ16_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45446.1"
                     /translation="VIKGITSGSEIHWLDNNRVAYRKGWTGNFSAVLYAINTGKSYPL
                     VRQSFNTNLSYSNNGKNLAILKDQVIMLHDLATGMTTSTGLEGEDLSFDPHGRYFTSL
                     LFKKLFVVGIGTVHNRRIELQRAWKTIAQVYESIRNDSSAFENEYSSEYVKRKIALYR
                     SLLRD"
BASE COUNT        13187 a        22086 c        19918 g        12955 t
ORIGIN      
        1 catcatacac gagcccgtag cgggttccgt ccatcagccg gtgcaggtac tcgccctgca
       61 gcgacacgta ccggtacgag tcgatcaggt acttgagcgt gagctcgccg ccgtacaccc
      121 aggtgtgcgc cttcaccgcg tggcgtccct cctcctgatt gtcctcgtcc agctcgtggt
      181 tgatgcgggt ggtcccgttc gccgcggagg cgccgatcat caccgtgaga tccccgatgt
      241 cgaacgaggt cttcacaaac ccggtgtaca gattgggccc gttgctgccg tcgatatcga
      301 ccgacgccgt ctcgccggtg gcgtcgacga tgggcgttcc gccggcatgg gtctgaaagc
      361 cctcgctccc gaagctcgac tcgttctgcc cctgaagcgc ctcgcccccg acgagcagat
      421 agaacccgac cggcgcaagc cacgtcagct gcgcaccgac ctcgttgatg ccgtgcgcgc
      481 cgaagaacgc cagataggga aggggctgct cggaaaaatc ccactggtgc gcgtgctggg
      541 cgttgatgcg cccgaagccg ctgaagaact tcccgccctt gaattgaaat ccgtacggga
      601 ggcaggtggt cgcgaaatac ccctcctcga tctcgacgcc ggtggtcgag aggtgcaaca
      661 cccccgtgag ttcgaagtag gggtccaccg gcgcaaacag gaacagctct ccatagttga
      721 ggttaaaccc gcgcttctcg ttcatcgccg cgtggctgtg cccgtgcccc tcgtgggcat
      781 gcgcagggcc gtgcatgaac tccggtattg agagcgccgc aaatttccgg tcctccaggt
      841 cgcgcgccac tgccgagaaa tccagaacga atgaaagatc gggaacgaat ttactgtgcg
      901 aaaacggggc cgatcccgat ggggcctcgc ccgcattctc ctttttccgc tcgggctccg
      961 cgggaacggt cgtctccacc ggtcccgccg gaccgtcatc gcgctgtgca atggcgctgt
     1021 cgcgggccag cgcgatgaat cccgcgatgc atactgctat cgcaatgcgt cgtatcatta
     1081 tcgcctcctg acgatcagat ggtcgcgcgc aaccgtccac atgttcgtgg cggtcgcacg
     1141 gaaaggggaa tgtgacgtca ggaagcgatc ggcggggcgt gggaatagta gggcggttgg
     1201 agcgcggccg tcgcgcaatc atacgtgacg aaaacgagcc gccgtttcac gacatgcgag
     1261 ccggcgtagg cgaaaaccgc aatgctggcc gtcgaggtca tgaggtgcat cacgacgcat
     1321 acctggcagt ccgaatgcgc ctttccgtcg tggtgatggt gcagcaggac gtgaacgacc
     1381 gacagcatga gcacgagcgc cgttcccagc gccgcggttc ggatcatcgt tgtcattgtc
     1441 ctggtcatct ctcgccgtct gcgtgtcgcc cgcagtcatc gcgcacccga ttcggaaacc
     1501 cggaggaacg cgcaccgcac cggccatcac taccatgacg cacccgggcc gtcaagaaac
     1561 ttggacgcct cggaattcac atttccttta tgttcccgat gcgccgtttc gatatatttt
     1621 gctttgcatt ttttcccgcc ttcccgctag agggaaaaat gcgttatcaa atgccacggt
     1681 atcttcgata ctagtagcgg gggaaacggc gccgtgcgcg gcgccgatct gcacggggcg
     1741 cacagcaggc acagccatgg tccgttcata cagcatcgag aacttccgga ttacccaatg
     1801 cgccgacgac ggcggcgaga tcctggtgta tgtcgatccc acccccgagg agcgcgcgct
     1861 cctggtcaac gaccacctca tcgacgcgca caccctgcaa tcgtcgttgg accccgacga
     1921 gctcgcccgg cttgaattcg agccggagca cgcggcactc attatcaagc gcccccagaa
     1981 ctattccggc ggcgaccagt tcctcttccg ggtttcgtcg tacggtatct tcctgttcaa
     2041 gacccgactc atcgtcgtga tcatggagga catcccgctg ttcgaccaca agctcttcac
     2101 gcgcgtctac tcgctccacg acgtggtgct caaaatcatc taccacacca tccaccacta
     2161 tctggaccac ctcaaggtga tcaacatgat ctccgactcg ctcgaggaca agatcaacac
     2221 ctcgatgaag aacaagtacc tcatcaacct cttcatgctc gagaagagtc tggtgtacta
     2281 tgtgaacgcg atcaactcga acgcattcgt gatcgagcgc ctcaagaaca acgccacgaa
     2341 gatcggcttc gcccccgagg gcgtggagat gctcgacgac atcatcatcg agaacaacca
     2401 gtgcttccgg caggcggaga tctactccaa cattctcgcg ggcctcatgg acgcgcgggt
     2461 gtccatcgtg agcaacaacc tcaacatcct catgaagacc ctcaatatca tcaccatcgg
     2521 aatcatggtg cccaccttcg tcgtgagcgc gttctccatg aacgtcgaga tccccttcca
     2581 gaagcacccg ctggcgttct ggatggtcat gggactctcc ctggtggccg tgctgtgctt
     2641 cacgctggtg tggcgcttta aaaaatggtg atgccccgcg cgaattgttg cttgccgttt
     2701 tcccgcgccg tgtgtagtat gtgaggtata ccagcaatca aggagcgcgc catggacgac
     2761 atgaatacac caacgtcgga ttccatcatc cagtcccatg tcatcttcgg gatgacggcg
     2821 ggcgccatcc cgctccccct ggtcgatttc gtggcgatct cggccatcca gatcgacctg
     2881 attcgcgagc tcgcgaagcg ccatgcggtc gatttcaacg ccaacctcgg cacctcgctc
     2941 gtctcgtcgc tcgcggggac ttcgctcgcg aagatcggcg cgagcgccgt caaggcgatc
     3001 cccggcgtgg gcaccgccct cgggatcggc gcgcaggtcc tgctcgccgg cgccaccacc
     3061 tacgccctgg gccgcgtctt cgattcgcac ttcgcgcgcg gcgggacgct cgcgaatttc
     3121 aatgtcgacg ccatgcggcg gtcctacgat gagatggtcg atacgggcaa agagttcgcc
     3181 caatcgctcc ggaagggcgc ctcgaagaag gaggacatcc tggccacgat cgaaaagctc
     3241 gccgagcttc gcgcacgcgg cgccatctcc gaggacgatt tcgagaccac caaaaagaag
     3301 ctgctcgatt cgctcgtcgc atgattgtcg accatatcgc cgccgcgcgc cggtacgatc
     3361 gcgggaccgc ctgggaggac gccttccgct tcctggcgac cctggacccc gccgcacgcg
     3421 agaacgaata cgccatccgc gggaccgacg tcttcgcccg tattttcagc tacgagaccc
     3481 ttccgcgcga acaggcgcgc ctcgaggcgc accgcgagta catcgacgtg cagatgatcc
     3541 tctcgggcgg cgagcgcatc ggctggtacc cgcggggcgg gctttccgtc agcgagccct
     3601 acgacccgtc gcgcgacgtc gagttctatg cggcggaccg cgagccgtcg tcctggctcg
     3661 ccctgacgcc gggcctgtgc gcgatctttt tccccgacga cgcccacctg acgcaggtcg
     3721 ccggcgacac cggtccgcag aaggtgaaga aggtcgtggt aaagatcaaa cgcgcgctcg
     3781 catttccctg acatcgccat gcgccgctcc gacaagaaaa tcttcgctcg tgacggtgga
     3841 ctgatgagcc tccgccgtcg catcatgctc ctgacgtgtg cgggcgtgct cacgatcgtc
     3901 ttttcgtcga tgggcgcggc gcagcagcgt gcacgcgcct tcatcctcta tgttccgccg
     3961 atggagaacc agggctcgcg cctgcacgcc tggttagggt tggccatggc cgatcacgtc
     4021 cgcgacatgc tctcgacgac cgacagcatc gccgtcatcg gcgaggacga gcggcttacc
     4081 gcattgcgac agcgtgccgt cgacgagcgg gcgaaggacc ccgatgccgc cctggtgcgc
     4141 gcgggccgct tcctgggcgc aagcgccgtc cttgtcgggc gctacacggt acggggcagc
     4201 gccgtcttta tcgaccgccg gctgatcgac tgccgaacgg gatcgaccct caccgccgat
     4261 tcgctcgcgg gcaccctccc gacgatctcc gatctccttg agcgattcgc gacatcggcc
     4321 tttcactccc ccgggcacgg cgacggggcc cgtaccgccc caatgacgct cacgactgcg
     4381 gaccgcgcat cgatcgccgc gtaccatcgc cccccctgga ccgcgttcga acactatgcg
     4441 cgcgggcgct cccttgaagc cgtaaagccc gatcgcgcct ttacgcactt catgaaggcg
     4501 atccagaagg atggattcta taccgaagcc tatatccagg cggccgctct gtgctatttc
     4561 aaactggacg atccggcgca ggccttcgaa tacctgcgcg cggccctcgt catcagcgaa
     4621 caacagcacg ccccggccag catgcccagc gcgggcatcg cgatgcgcat cggccagctc
     4681 tatgccgcac agggcgatct ggaaaacgcc ctgaagcact atgaggacag ccgtcagcga
     4741 tacgaaaaga tcaatcggca ggcatcgtat ccgtatatcg tcctgatggg ccacatcggc
     4801 gaggcccacc ggctcaggaa cgaatacgac cgtgccatcg agtccctcgg gcgcgcgaag
     4861 gagatcgccg aacagctcga ccttgccgac tccgagccct acgccgagct tctggcgcgc
     4921 atcgggacgc tgttctatca ccgcgaacgc tactcgaggg cggaggacct gtttgcgagg
     4981 agcctcgcaa tccgcaccat caacgaaacg gaacagacac ccgcattcgt gtccctgttg
     5041 aacggactgg ccggcatcta ccgacagcag aacaacccgt cgaaggggct gcggtatgcg
     5101 gagaagggcg aggcgctctg ccgcgatccg cggctggcgc gcaccgtcga atgcgccgcg
     5161 ctcatgtcca ccacgggcat cctgtattat ctccagcagg actacgcaaa atcggtcgcc
     5221 gcccacgagc acgggcggag aatcctcgat gcgctcgcac tgagcgacaa tgcgcgctac
     5281 gccgccgcgc tctatcatct gggcggatca tacatcaaga ccggcgatcg ctccgccgcc
     5341 gggcgctgct tccgcacggc tcacgacata taccggacgc tgggcatgca ggagcaggcg
     5401 gagatcctca ggccgttctc ggagtattac gggaagggta cggagtagga aggctatcgc
     5461 acgatccgca tggagaggtc gaaggccttg acggagtgcg tgagcgcgcc gatcgagacg
     5521 taatcgatgc ccaggtccac gagccgaccg atccggtcct cgtccaggtt ccccgacacc
     5581 tcgatcttgg cccggccggc gatcccgtcg atagcgatcc gcatggcctc cttgtccatg
     5641 ttgtccagca tgatgacatc aacccccagc gcggcggcct ccgtcgcctg ttcgggatcg
     5701 cgcgcctcca cctcgaccgt gaagcggtcc ccgtaggcgt cccgtacgcg gcggacggcc
     5761 tcggtgatcc cgcctgcggc gtcaatgtgg ttgtccttga tgagcacggc gtcgaacagg
     5821 ccaaagcgat gattgccgcc cccgccggtc cggaccgcgt acttgtccag cagccgaaaa
     5881 ccggggaggg tcttgcgggt gtccagaacg gtgataccgg tgccccgcag cagcgacgtg
     5941 atccgccggg tcgcgcttgc gatgccgctc atgcgctgga ggaagttgag cgcggtccgc
     6001 tccccgataa gaaggctctt cacgctgccg ccgagacgcg cgaccgcgtc cccgggcccg
     6061 accaggtccc cgtcattcgc gaggatatcc accgaaacct gctcgtcgac gtggcggtag
     6121 acgcagcgaa ggacctcgcc gccgcagaac acgccatccg ccttcgcgac gatctccgcc
     6181 gcgcccacgc gcggctcgcg gaacaccgcc tccgaggtga tgtcgccgtc gccgatatcc
     6241 tcgcgcaagg ccagcatgat gacgggcatg atatcgcgct ccgcgatgct tcgcttcatt
     6301 tcaccttttt tcgcaggtcg atccgcttct cgagctccag catctggtcg cggagcacgg
     6361 cggccttctc gaactcgaga ttgtcggccg cgccgatcat gtcggcgcgt atccgctcaa
     6421 gcagcgcgag ctgcgccgcc ggatcggcgt cgcgataggc ctcccggtat tccgcgacat
     6481 acgacagcgc ctcgtcggcc gggtggtact cgcgctcgat gatgtccccc acctctttcg
     6541 cgatcgtggc gggggtgatg ccgtgctctt cgttgtattg ccgctggatc gcgcgccggc
     6601 gctcggtcgt ttcgatggtc gttcggatgg agtcggtgat ggtatcggcg tacatgatca
     6661 cggtaccgtt cacattgcgg gccgcgcggc cggcggtctg gataagcgag cgcgccgaac
     6721 gcaggaagcc ctccttgtcg gcgtccagga tcgccaccag ggacacctcc ggcaggtcca
     6781 gcccctcgcg cagcaggttg atgcccacca ggcagtcgaa ctcgccccgc ctgagatcgc
     6841 ggatgatctc gacgcgctcg atcgtctcga tctcggagtg cagataccgc gcgcggatgc
     6901 cgatctcgtc gagataggcg gtcaggtcct cggccatctt cttggtcaat gaggttacca
     6961 gcacccgctc gcccgcgtca atccttttcc ggacctgccc gatgaggtcg tccacctgcc
     7021 cggtcgccgg ccggacctcg atcaccgggt ccaggatgcc ggtgggccgg atcacctgct
     7081 ccaccaccgc ctcgctcacc gagagctcgt agtcggccgg cgtcgcggag acgaacaccg
     7141 cctggtcgat catggcctcg aactcctcga agaagagcgg ccggttgtcg agcgcggacg
     7201 ggaggcggaa gccatgctcc accagcgact cctttcggga gcggtcgccc aggtacatcc
     7261 cgcgcacctg cggaatggtc acgtgcgact catcgacgaa gagaagatag ttacccttga
     7321 agtaatcgat catgcaatcg ggacgggagc ccggcggccg gcgcgcgatg atgcgcgaat
     7381 agttctcgat cccgctgcag tagcccatct cctgcagcat ctccatgtcg tacttggtgc
     7441 ggctctcaag gcgctgcgct tcgacgagcc ggttctgcgc ggtaagctcc cgcaggcgcg
     7501 cgtcgagctc ctcctggatg aggcgcacgg tttccgagag ctcctcggcg tcggtcacga
     7561 aatgcttcgc cgggtagaga tactcggaat cgatctcgtc gatgatcttc cccgtgatcg
     7621 ggtcgatgcg gtacaatgcc tcgatctcgt cgccgaaaaa ctcgatccgg accgccgtct
     7681 tcaggtaggc cgggaacacc tccacggtgt cgccgcgggc gcggaagatg ccgcgcgcga
     7741 actccacatc attgcgctgg taatggatgg cgatgaggtc ccgcagcagc cggtcgcgcc
     7801 ccatatcgtc cccgcggtgc agcgcgatct gcatcgcctc gaagttctcg gggctgccca
     7861 ggccatagat gcacgatacg gacgaaacga cgatcacgtc gtcgcggtcc aggatggacg
     7921 aggtcgcctt cagccgcagc cggtcgatct cgtcgttgat cgacgagtcc ttttcgatgt
     7981 agaggtcctg cgccgccacg taggcctccg gctggtagta atcgtagtac gacacgaagt
     8041 actccaccgc gttctcggga aagaactcct tgaactcccg atagagctgc gcggccaggg
     8101 tcttgttgtg cgtcagcacc agcaccggct tgtttaccgc ctcgatgacc ttcgccatgg
     8161 tgaaggtctt tcccgaaccg gtcacgccga gcagggtctg atatttcatc gttccgcgaa
     8221 tgcccgcggt gagtcgctcg atggctgcct gctggtcacc ggacgggcga aagggcgata
     8281 tcactttaaa tggcggcatg atcggtgacg tatacaataa tcgaaatttg accgcttggc
     8341 gcaatatttg attgacggat gcgctgtgcc gtgtatggta cgcagcgata gggcactatc
     8401 gctcctggtg cggttttttt caatcctata ttctcccggg ataccgtcgg atacgatttc
     8461 gagggccgcc atgttggaca tcagcatcga ccgcaaggac cgcatcgcca tcatgcatat
     8521 cgccggcgac ttctacatcg acagcatcgc cagcgcggag cgctcctgga acgaggtcgt
     8581 cgccgcggaa ccgcggatca tcgctctcga ctgccggaag ctcgcgcgca tcgattccac
     8641 cgccatcggc acgctggtga aattcttcaa catggccatg gcgaaaaaga tccgcctggt
     8701 cttttgcgac gtcaatgcct ccattcagct cctgttcgag acggcccgtc tcaagcggca
     8761 tttcgtcatc accagcgcga aaaaattcaa cgacaagtac ctgcggcaaa tcgcctgagg
     8821 cgcgtacatc cgtcatgttc ctcgtcacca tcgaccaccg gggcgccgtt ccgatcctca
     8881 acctcaaagg cgacctcatt ctcgattacg tggagcatgt cgatgaggtc tggaaccggg
     8941 taaaggaggg ccagccgcag gccatcgcgc tcaacttcaa agacctcgaa cagatcgatt
     9001 cggccgggct tggctccctc atcaggttcc actacgaatc gaaggataaa aacatcgagc
     9061 tcatgcttct ggatcccagc cccgagatac tgagcctctt tgaggttgcg ggcattcata
     9121 tcctgttcac catcgtcacg cgcgcgcagt tcgaagcgga ccatcccgcg taacaccatc
     9181 gcactcataa acggaggtac cgatgaaagg aataataatt ctgacggcga ttatcgcgtg
     9241 ttcgtgcgcg atcaactgtt cgtcatcgaa aaaggtcggc tgtgtcgacg gcgattgcaa
     9301 gtccaagaac agcgcgtact actggaagga cgggtcacac tacatcggcg ggtacgtcgc
     9361 aggggtcccg tcgggcaagg ggacctatgt atgggcggac ggaaaaaaat acgagggcga
     9421 catgctcaag ggacagccgc acggccgggg cacgcttgtc ttccccaacg gcgaaaaata
     9481 cgagggcgac attcagaaca acgtgttcgt cgggaagggg acctacactt ataaggacgg
     9541 ccgcgtctac acgggcgagt tcaaagcggg caagccggag ggcaccggca tgatgacctg
     9601 gcccgacaag acgaaatacg agggccagtt taaaaacggc accatggagg ggaagggcgt
     9661 ccggacgctc ccggacggct ctaaggaaaa ggggaccttt ctgccgggcg gtattttcgt
     9721 aaacgactga ccgcgcacac ccggtgcggc tacatcagga agccgcaccg gtcgcatttc
     9781 accgtctcgc cctcaagaaa gatccttccc agcggtgcgc cgcagctcgg gcaaaacttg
     9841 aacttcacgt cctggcgccg cgcctcctcg gtaaagaact cgtcggtggt ccggtcgaaa
     9901 ttccccgcga tcctgccctc ggcgatcgca tgcgcgagca actcgctcgc ctcgggtatc
     9961 gatacgccga tctttcccgc gaggtcggcg acggtgatcc gccggtagga tttcaccacg
    10021 ctcgcgatcg actccagccg ctccaagcgc cgccgcgcgc gccggaagaa atacagcagc
    10081 accgcgcccg gcgcgaggat ggacgtggag aacatcgcga ccccccgtcc ggtttcgctg
    10141 tggacgggat catcgtcgaa catcatcgcg acacccacca cgaggacgaa aatgcccgca
    10201 aagagcgtca ggaacccgaa gcccttctgc aggctgagtt tcaatgagcg caccatcgcc
    10261 gcctcccacg gtaccgcata tcaatacaac acccgttcga tgctccggtc attcatggcc
    10321 gcaagatagt cctttttcca gtccggcccc aggaagcgct ccgccagatg gacggggagc
    10381 gccatgcccg tgaccgcgac ctcgccgttg atgcgcgaca gcccctgcac gcagctcggg
    10441 caggtggtga ggatcgtcac ctccttgccg aggacggcat cgcccacgca gcgccgcttg
    10501 cggtcgcgga tttggttgga tatctccggc gtggagagcg caagcgtgcc gccctcgccg
    10561 cagcagtagg ggatgttcac cacgcgttcg ccggtgaggg acgagatgac cttctggtag
    10621 cccagctgct tcaacggcgt gtggcaggga tcatgatagt acaccacgcc gtccgtccgt
    10681 ggcgccgatt cgaagaaccc ctcccgcgcc agaaactcgt tgatgtcgat gagccgggcc
    10741 ccgttgaaga tattctccac ctcgtagccc tgcagcatct cgaagcaggt cccgcacgat
    10801 acgaccacat tgtcgatctc cagatagttc gcgacgtccg cgatcgtgtg gaagatcacc
    10861 cggttctcat agctcttgat cgccgcgctc tgcgatttcc cgttggccag caccgggtag
    10921 ccgcagcaga ggtactccgg aggcatcacc acgcgcacgc cggccgcgaa caagagcgcc
    10981 tgcgacgcga tgctgatgtc ggggaagagc cgttccgacc cgcaccccgg gaagtagaac
    11041 acgctcttac cggacgagcg cgtcgggttt tggatgataa tgaaggcgtt cttccgcgtg
    11101 acgccgatgt gctccttgaa ggtcttctgc cccgcagcgg gcagccgagt gcgcaccagc
    11161 gccgcgatcg tgggcacaat ggcgcggatg acgcggcgcg ccggcttgac ggcgtaatag
    11221 gcggcgttct gcatccggta gccgatccta aacatcagca ctcgaaagag cttgttgatg
    11281 taatagccct tccgcatgag gtaaaacagc gagagcgccg tgagcgtctt cacccgcgac
    11341 acgcgccgct cggtgaggat ctcgcgcatg ttgagcgtga tgtcgccgaa gtcgatcttg
    11401 accgggcagg ggattgcgca gcggtggcac atggtgcagt agttcgcgat ctcgatgagt
    11461 tgcgcgaagt gccggaagtt cagcgacttc gacatctgcg cctcgtagag gaccgcctcg
    11521 atgatgagcc ccacgcccag gatcttgttg cgcggattgt aaaacatcgt ccgccccgga
    11581 tgatgggtgt tgcaggtccc cttgcacttg ccgcaccgga cgcaggaggc gatcgacgtc
    11641 gagagcccct cgaggtcggt cgccttcagg atgaacgcct cgagcttgag caggttgaac
    11701 gagggcgtgt agatggtgct caggggaaag gcggcgttga gcttgccggg gttgaacagg
    11761 tcatcggggt cgttttcccg cttgtatgcg gcgtagccgt ccaggatgcc ctgatcgaca
    11821 aagcgcagct tcgtgaggcc gatcccgtgc tcgccggaga ccacgccgcc cagccgcacc
    11881 acctcctcca tgatcacgcc cacggtcgca tcggcctccg ccatcatcag gtagtcgttg
    11941 gagtgcaccg ggatgttcac gtggacattt ccgtccccgg cgtgcatgtg agtggcgatg
    12001 atgatcttcc gcttgcgctg cgtgtcggcg gcggcgtcga aggcgcgcat caactcgtcg
    12061 taattatgaa aggatcgccg gaatggctcc gccacatcgc actcgtagtc gacccgaatg
    12121 accccatcct gcaagaggcg gaacaccgag cgccgctcgg cggacagcgc cggatcgtcc
    12181 gtcaagacat cgcaggccgt ctggttcatg ttctcggcgt agcgcagata ggtcgtgcgc
    12241 acggcagtgg catgcgcgcg gtactcgtcg atgcgtgcgc gaaggaattc gtccgcatcg
    12301 tcggtgtcgc ccgccgcggt aagcatggcc tcgacgtccg cgacgatctg cgcgctattg
    12361 gacagctcct tgaggaggtt cagcttgtcg atgaagtccg cgaactgcgg cagctgttcg
    12421 atggggatga cgatgtcctc gttgagcttg aaggcgttcg tatggcgcgc gatcgccccg
    12481 aggtttttcc ggtccttcca gaagttctcg cggtgcgccg gcgtcgaggc gatgaacccc
    12541 tcggtattgt actcgcgcac gagcgcgatg gtgcgcacgg tcgcggcgtt caacagcgcc
    12601 tcgatattgc tctcgatgtc gatgatgagc accgccttgg ggacctcgtt gcgcttcgac
    12661 ttgttcttgt agttgatcgc ccgcacgtat ttgtcgtcga agtgctccag cgcggtgagg
    12721 tagacccggt cgttctccga gaagtgctca atgatgtcca cgatggcctt cgaggcgttg
    12781 atcatgttat tgccgtagaa ttcgaggcac accgtgcggc aatagtggaa cggcttatag
    12841 agcacgaagc tcgccgagac gatcaccccg tcgccgccct ccttctggag gccgggcaac
    12901 ccgcccagcg ccttgttggt gatgtccttg ccgagcccct ttttccgcac gtcggtgccc
    12961 gaaagttcga cggtccgcag acgctcgcgc tggccacgtc gcttcaccac ggacacgtcg
    13021 aagatgacgc ggtctcccgg ctcgatcttc cgataggcgt gatcacgccg tgcgacctcc
    13081 accagcgagc cgtcggccgt gacgatccgg aacgagagga ggttgtcgat tgcggtgccc
    13141 cacatcacgc acttctttcc gccggcgttc tcggcgatgt tcccgccgat ggtggaggcc
    13201 caagccgagg tggggtcggt ggcgaagatg tagccgcgct cggcgcagta ctccatcacg
    13261 tcctccgtga ccgctcccgc ctcgacgttc accaccggga cccgcgagcc cgcgatgtcc
    13321 atgaactcga tgccctcgat ccggttgagc cgctcggtgt tgagcacgag cgtacccttg
    13381 cgaacgggca ccgcgccggc ggtcaggccc gtcccgccgc cccggggaat gatcgaaagc
    13441 cccaaacgcc gcgcggccct gacgatatgg ggcacctcgc gcgcgcggct cggatacacc
    13501 acgaccgacg gatattccac gcgccagtcg gaggcgtcgg tggcgtgcga cacgcgatgg
    13561 aagggggtga agtgtatgtt cgcgcggctg gtcgcatgcg acaggaccga ctggatgcga
    13621 gcgcgccttc gccgcgcgcc gccgagctcg tcgaagaact cgttgccccg cacgagcgtc
    13681 tttttaacca ggtccagcac cagctggttc cccagtgcgc cctcctcaac cgcccgcaac
    13741 cgcatctcgt gcagtcgccg gagccgcttt cgcttccgtg gattgtacag ataatcgtcg
    13801 tagaggtagg ggttgcgatc gatgatgaag atgtcgccat agatctcgaa gagcaaccgg
    13861 gcgctgcgcc cggtcacgcg gtggctgcgg agctggtcca gcatgcccca ggtctcctcg
    13921 tcgtaatact tgagcacgat ctcgcggtcg gagaacgagg tatagttgta ggggatctcc
    13981 cgaaatttcg aatccttgat catggcgcca tccgctttcg aaggccgtat cgcggctttg
    14041 tagtggtgca tcaggcatcg gtcgtcaagt gaatttatca cgcctttccc aaaatatcaa
    14101 aacatatgaa aaatccgttg ccgaaaaaac tttttcaaaa taggacggtt gatggcgccc
    14161 cgacgcacgc ggggtgcgcg catcgagtct cggaccccgc cgcagtacgc gatgcggacc
    14221 gagcgcggcg cagggcgggc aatggagcgc atggaaaccg cacccacata tctgctgatc
    14281 atcaatcacg ggcgcgagcg cgggtacgaa acccgcgagc tcgaacggga cgacttcatc
    14341 gtccacacat cgcgactgct caaacgaccg cggaaggtct ttctgggcct gggcgatttc
    14401 cttgaggtcg gcgactataa aaagctcatc gactccctct ttttcaacat ctccggcagc
    14461 gccatcgaga cgctgctgga cgacagcaac cggcaggtct cggccgagga gaacattccc
    14521 ttctcgtttt tcaagctcgc ggtctatttc ccggatttca tcttgcgcga catcccgacc
    14581 tcctgcgcct cggtgatcgg aaagaagggg cgcctctttc cgggagcgga gaacttcatc
    14641 aaacacatca agccctatga tccgctcatc cttacggcaa tcccgcacga gatcgccatc
    14701 gagttcgtca agcggctcga tctggaggaa cgaaacctca tctccacgcg ctacgcggtc
    14761 gcgaccaacg ccaaaaatcg agaggtgtac tcgggcggcg tgcgcgtctt catgtcgggc
    14821 aaccgacgga gcatcgagat cgagaaggtc atgaaccgcg agaacctctc gggcgaggac
    14881 atcgtgtaca tcggtcgcgg cgaggcgggc ttcaatgctt tcgccaatat gaattcgatg
    14941 gccttcaacc cgacgaaaag catcattccc gaatccacca tcaccctgta cggctcgtcg
    15001 ctggaatcgc tgttggtgtt gttcaacttc gggggtgcta tcactccgct cctcaccgca
    15061 gacgccatgg aggcgaacct gccgtcgctg gtcgtgtttt ccgaggagcg gtcgaaacgc
    15121 gacgagctca tcgagctgga gctctcccac cgccatctgc agaacaacat cattggccag
    15181 cggctcgagc attccggcga gagctacgtc tcggtggagc gcgagatcga catcgagttc
    15241 ggcggatcgt cgatcgacat ccagggggtg cgcgacatgg tcaagaaccg gatgagcgca
    15301 taccgcgacc atcctcagga tttcgtgcac gaggtcacca atatcgcgcg cgagcgctac
    15361 ggccgtctct attcgtcacc ggaggatgaa tcatgatcac gattggcgtt atcccggctc
    15421 gctacggatc gtcgcgattt ccgggcaaac cgctggcccc catcgacggc gtcatcctca
    15481 tcgagcgggt gtaccggcag gcgcagcggg ccaacctcgc gcgcgtcctg gtcgccacgg
    15541 acgaccggcg catcttcgac gccgtcacgt ccttcggcgg cgaggccgtg atgaccggcg
    15601 aacacccgtc cggcaccgac cgcatcgccg ccgccgtgcg cggcatcgac gcggacatcg
    15661 tcgtgaacat acagggcgac gagccgctca tcgacccgcg cgtgatcgcg accgtcgccg
    15721 cggcgctgga gaacaacgcc tgggccgaca tgtccacggc agtcacgcgg ctcgatgcgc
    15781 ccgaggacat cgacaacccg aacatcgtca agtgcgtatt cgaccaacac ggccgcgcgc
    15841 tctatttctc gcgatcgcgc gtcccctaca accgttccgc gaccgatcac taccacaagc
    15901 acctggggat ttacggatat cgaaaggaat tcctcgagcg cttcatctcc ctgccgcaat
    15961 cacgcctcga ggcggccgaa tcgctggagc agcttcgcgc gctggagaac gggcatgggc
    16021 tccacgtctc cgtggtcgcc tacgattcga tcggcgtgga cgtgcccgaa gacgtggagc
    16081 gcgtggagcg gatccttcgc gcgggccgat aaccctgccg cccgttcact ttttttcttc
    16141 gaagaatcgt gtcccgtccc actggtagac ggtgaccgtc gtgcccttga ggacgtattc
    16201 ttttttctcc tcgtcaaaat cctcgtcctt gcgcgtgcac acgattcggt ccggacgacc
    16261 tcccttctgg tttgggaaca ggcataccgt gcccatgtgg aacacgccgg cttcgctcac
    16321 ggtgaccgcc gaacacgcct caatgagcct cttcccgtcg aacacgaaaa acatgctccc
    16381 attggcatac gcgcacgcgg ggtattcgaa cttcagctcg ataatgccct tcacaccgct
    16441 cagttttctg ccgtcgtgca tcgtgccgta aacggaatac ccgaattcgc ccgttccgct
    16501 cacgtccgta tagatcggag cgtagacgat acgggagatc gtccttcccc ctgaaagcgc
    16561 cgcgatctcg gcgaaatacc gcccgtccga cccgcgatgt ttcaggccgg acacgaagag
    16621 cacctgcgtg ctgtcgatct tcgcaactcc atgcgcatac gagaagaagc cgccccaggc
    16681 gtaaccggtc ttctgtgcgc ccttttcctt aaactggatc tgataccagt tcgcggtaaa
    16741 accgttttgc gaaagccgtt ccaccgattt tttcacgatc cgtatcgcat ggccgtacgg
    16801 gaggtttgcg atcacctcgc tccccttcag cgatggcgca ctgcggacat ttaccctgtc
    16861 ggcgcacaaa aaggtctggg cgccatcagt gaaatcgccc atcgagtaaa caatgtgttt
    16921 ttcagcatac gcgcgcgatg cgcatatcgc acttatcata atgagagcga acaaccgtat
    16981 cgtacgtttc atggattttc cctcacgagt tgcatcgcgc gccctactcc tccggcgaat
    17041 cgaacagacc gccggactcg ggccggtcgg cggcgcccgc gaacgggatc cccaggtgcg
    17101 tataggcgct tcgcgtggcg acgcggcccc ggggcgttcg attcaaaaac ccgatctgca
    17161 ccaaaaacgg ctcgtagaaa tcctcgaggg tgtcgatctc ctctcccacc gccgctgcaa
    17221 tggtcttgat cccgaccggc cccccgttga atttctcgat gatgagcgac aggatccgcc
    17281 ggtccatctc gtcgagccct atcccgtcca catcgagctg ctcgagggcg tagcgggtca
    17341 ccgcgaggtc gatcgcgtcc ttccgctgga ccgtcgcgaa atcggcgacg cgcttgagga
    17401 ggcggttcac gatgcggggc gtgcgccgcg aacgcgaggc gatctcgatc gccgcgtcct
    17461 cgcggatcgg gcggcccaga atacgcgccg agcgcatggc gatcgtttgc agatcggcgg
    17521 actcgtagta gttgagccgc agcgggatgc cgaagcgatc gcgcagcgca gcggtgagca
    17581 tgcccaggcg cgtggtggcc ccgatgatgg tgaacggcgc aaggttgatc ttgatcgtct
    17641 tggcccccat gccctgtccg atgacgatgt cgagcgtgcg gtcctccatg gccggataca
    17701 ggatctcctc tatggcgggc gcgagacggt ggatctcgtc gatgaacagg acatcgttct
    17761 cctcgagcga ggtgagcagc gaggcgatgt cggacttttc cagcagcggc gcggaggtcg
    17821 ccttcacggc cacccccagc tcgttcgcga tgatgtacgc cagcgtcgtc ttcccgagcc
    17881 ccggcgggcc cgaaaggatc acatggtcca acggttgccg gcgcatccgc gccgactcga
    17941 tgaacacccg caggttcgcg acgctcttcg cctgcccgat gaactcgtcg aacgcgcggg
    18001 gccgcagcga cagctcgtcg tcgtccgtgt ctgttttgtt cggaaccatc agccgggtgg
    18061 tgtgatccat gacgcctact tcttcgcgtt tttttcgaag ttccgtatgg ccttgacgat
    18121 cgactcgggc gaatccagtt cgagcagcga ttgccgcagg gcgtcgttgc tgagcacctt
    18181 ggcgatgttt gcgagcgtct tgatgtgctg ggtgagtttg tccttgggga cgatgagcag
    18241 gacggtgatg cgcaccggca aattgtccac ggcgttgaag ttgacgccga tatcggtgat
    18301 cgcgagcacg agcacgatat cgtccacctt gtcggtgctg cagtgcggaa tggccacgcc
    18361 cttgccgatc ccggtgctca tcgacttttc tcgttcgaag agcgcctttt tgatctcctc
    18421 gccgtcctcg ccgcggacct gccggttctt cacggccgcc tgcagcagct ggtcgatgag
    18481 ctcccaccgg ctccgcgcgt ggacgcccgt cagcacattg tccttgttga gatggtccga
    18541 tactatcata ggtgttcggg tggtccctgc gcgccgacgg cgcgaatcat tgaccgatcg
    18601 tcgcccctgt tttccttttc gtcaagagtt ataatttttt ccgtttcgct gccgccgcgc
    18661 gcacccggtc cgcgataaaa ctcacgaacc cgcgctcatc ctcgaggacc acgtcgacgg
    18721 gcacatacca gagcgcatcg ggaagcggaa agcgccgcag cttgatgatg tccttctcgg
    18781 tggtcagcac catatcgacc ccgcacgacg tcgagaagat tcgatcgacg tcttctttcg
    18841 aatacgcatg atgatcggga aacgccatct cgtcgatcga tgcagggcgt tgcgcaccca
    18901 ccagcgcgcg aaaccccgcc gggttcccga tgcccgacac gaggagcacc cgcgatcccg
    18961 caatggattc gatcggccgc gtgtcccccg catccgcacc gcggcacagc cgacgcggcc
    19021 ggtaccgtcc cgtcgtcatc gggcggccgc gtgagatccg ccgcacccgc gcgagtatcc
    19081 gctcgcgtct ctcgtcgcga accaaatcgc atttattcag tatgatataa tctccccgcc
    19141 gaagggccgt cgccggttcg cgcaggtttc ccgcgggaag cgccagcccc ccgccgaagg
    19201 gatcggtcgc gtccaccacg agaacggcca catcgctgcg caccgacgtg ttttggaacc
    19261 cgtcgtccat gacgatgcag tcgacgccaa accggtccac gatggtgctg atcgcgttcc
    19321 ggcggtcggc tccgatcacc accggcacgc cccgaagcgc gcctgcgatg agcaccgcct
    19381 cctcccccgc cgcatcggcc gacaggagca cccgctcccc gtcgcccacc accgcgcctg
    19441 cggcggagag gcgcccgcga tacccgcggc tcaccacccc gggccggaaa ccgttctcgc
    19501 gcagcgcgct cgcggtgcgg atcaccaccg gcgttttccc ggtcccgccc gtcgtgatgt
    19561 tgccgatgca cacgaccggc acgggactgg accacggacg cgcgcacaac cgcttcacgt
    19621 agaaacccag cagatagagc agcgatacgg gagcgaggat gacgcggagc gcgcgaaata
    19681 tcttcatacc ggggaggttc cttgcgcgcg attaatgata cataatctga aaaagtcaat
    19741 cgatttccat cgtgagaatt ttaattgact tttatcgccc gcccggccca actacggatg
    19801 agacgagcgc gaaacggcgt gcggaacatc tcgacggtaa atacaggttc atcatgcggg
    19861 gtagaccatg atcgaaataa aagaccaggg aaacggcgtt ttcatcatcc atataaaaat
    19921 gtccgaggtg catacgctcg atgtccccga gctgaaggaa aagctgcaaa aggccattat
    19981 cgacaagggt atcaaaaaac tggtcctgga cctctccgag gtcaagatga taaccagctc
    20041 cgggatcggc atctttttaa acatcaatca aagcctcaag tcgggtcttc gcctcgcctc
    20101 gccgaatgcc gaggtgcaaa aggtcctgga gctcaccaag gtttcgtccg ttattaaaat
    20161 tttcgatacg gtcgatgcgg ccatcaagag tttttaacac ctcgctcttg acgattgaga
    20221 aacgattgag tatattgttc atgagggacg cacgaagaat cacaactgta cgttaacgct
    20281 tgcaccgtgg ggtgcgcact gggggcacga gagactgccc cgcaacgaag tacgtgaaac
    20341 gatgggggga aagcgatggc gttcccccca tttatttata tcatgcagca caacaccgcc
    20401 tatatcgaca tcaccgtcaa accccgctcg tcccgcagaa tgatccgcgt cgaggacgac
    20461 agccgcatcg tcgccttcat caacgcgccg cccgtggacg gaaaggccaa tgaggagtgc
    20521 atcgagttgt tctccgatgc gctcgggatc gccaaatcga gaatctccat cttccgtggg
    20581 caccatggcc gtcggaagac cctggcgatc gacggcgagt cggcggaaac cgtgcttcgc
    20641 tcgcttcggc catgatcgcc cggattgcac gcatcgccat ctgctgcctg atcgccgcgg
    20701 tcctcgcgac atgcggcggc cgttcatgga cggtggccga ccgtcgcgag gggataacga
    20761 aggaccagtt tcgcgtctgt gtgcgcgtcc cccgcgggtc cctctccggg atcgatccgt
    20821 caggcctttc gccggccctt cgccgccatg ccgatgaccg cttccggacg ctcgtcggcc
    20881 atctcagcgc ctcccgccgg agcatcgcga atgataccgc gcgattggac gccctgatgc
    20941 cgggcatcgt caccgcgggc gtcatcgccc atcagtcctg cgagaaagat tactgcgagc
    21001 tctttgtcga ttacccggcg aaggagcttc gcgcgctcgt cggcgaatga cgcgcaccga
    21061 ccgctacatt cgcatcgaca ccggcgacgg cacatattcg cttctggacc gcgagctcga
    21121 cgaggccatg cactcgcgct cgggcgccta tgacgaggcc cttcgcaagc atgtcctccc
    21181 atcgcgcgcg ctcgaggttc cgcgtgccac cgcgcgcgtg ctcgacatcg gcttcggcct
    21241 cggctacaac atcctcgcgc tcatctgcga gcgcgccctg cgcgccccgt cgctgcgatt
    21301 ggaaatcgtc accatcgaac gcgacgcaga gatctttccg catcttgccg atgttcgctt
    21361 tggcgacgat cgcgacgccg tctacgacaa tatccgcacc ctggcgaacc ggggcattgc
    21421 ggacggcccc gggtataccg ccatactgcg gctcggcgac gctcgccaga ccatccgcca
    21481 tgtcgatggc gcgccgttcg acgccgtctt tctcgatccc tactcgcccg cgaaaaaccc
    21541 tgagttatgg acggtcgact ttttcctgct actgcgcgct ttggttagcg acgaggcgat
    21601 catcaccaca tactccagcg ccccgcaggt gcgcgaggcg ttatgcgaag cgggatttcg
    21661 cgtcgggccg ggaccgtcgg tgggaggaaa gcgggagggg accctcgcga ccgtatcggg
    21721 tcccataccc aaactcacgg cggaggcgtt ggaacttctg cgtgacgatc cccgatcgac
    21781 gccgtaccgc gatcccgggt tacacagcac gcgtgagatg attcgcgccg ggcggcgcga
    21841 tgagatgcgc agccgccgcg cggttaaaaa agatcgtcaa gctccttgat caccgagggg
    21901 ttctcgcctt ccgtggcggc atcgggcgcc gccttgtccg cgcggtcctt ccggttcgtt
    21961 cccgcgcttt tgtcggcaat cgctgcgcga acgctccggt ccgaatcgga gaactcgaga
    22021 tttccggaca gatcgccctc aaagcccagc cgataggcga acccggccgt gtactggtac
    22081 accagggtga tggcctcgta gattttcttc ttgtccagct tcttgttccc ctcgatctcc
    22141 gcattgaacg ccagcggatc ctgcgggttg tccacgatgc cctgcttccc gttcatgtcg
    22201 gcgacgatct cggccagctc cgccgacacc ttgttcatga acatccgcgc cgacgcgatg
    22261 gcgttgcgcc ctgcctgtat cttttccttc tcaaggctcg tcaggttttt cgaatactcg
    22321 atgtattgcg cattgctggt gggtttgtcg cgctgcgcct tcgcgaaggt cgccagggac
    22381 tccgcgtagg cccgaatgcc ttcaaggcat ttgagcatct cattataggt gtcggagagc
    22441 accgcgcggt aatcggtctt cccgccggac gaatacacga cattgtattt gatcttgttc
    22501 gtcgtcagaa tgatggagta ttccttgtcg aactcctgca tgagcagata ggtgacgagg
    22561 accttgtcgt tgtcgctcgc ctcccggaag atgtcggcgc ggtcgtagtc cttgcgcagc
    22621 cgggcaagat cgagctggaa catcatctcc acgccttttt tgatatgatc cggcagctcg
    22681 gcctccgcgt tctcctcttc ggtctcgcgc gcgccggcct ccggcagctc gatttccttc
    22741 ggctggtccg cacgcacgcg gttccccacc cgctcctcgt cggtgacgcc cagcagctcc
    22801 tccagctcgc cgccctgcaa cgggagaacg cgcccctgga tgccgcagaa cagccagtag
    22861 agctcctggt agagtcggtt ataaatgaca aacacgctgt ttttcgcctt cttcctcttc
    22921 cgcgaataga ccgaggcctt ctccttctcc agcttcattt gcatggagat ggcgcggtcg
    22981 aaggcgtact ggagctgctc ctggtaggcg acgatcggta cgagccgccg gaggagcgcc
    23041 gtgacgtgct cctggaccag atcgagccgc agggggaccg tgtgcgattc ggtgtagttg
    23101 ttgaggatgc gatcgacgat gcttcgctcg taaatcgagg agacgcgctc gataagctcg
    23161 aaataaaccg gcttcacgct atccaggtcc tcgatgatgg cgcgcgatac gaccggattg
    23221 ctcttgaaca catcgagata caccatctgt atctccatga gcgctgatcg gtattcgaaa
    23281 ttgatccgat tgaggaattt cggcttgagg ttcagtatgg aaaaatcggt cacgcccatg
    23341 aacagcagtt tgaagcgaat ggatagacgc tcgaagaatc cgccccctgt ggcgtcgcga
    23401 cctttggagg cagccggctt cttttttgaa gggggtgatg atttcaccgc cccgacgggc
    23461 tgacgggtgc ccagcttctt tttatgctct tcaattttcg tcttgtaggc gcgctgcttg
    23521 tcgcgatcga agtcggtcat gccccgccgc ttcttctcgc tgacgacctg tccgcccgcc
    23581 tggacgaatt tattaaagag atccttcttc gtgtggtcgt ctaagttctt gacgccgata
    23641 ttgcttttcg tcttttcgaa atccttgccg ctcataaccc gcccgcgcga tgcgggcccg
    23701 gaccccctgt gctctcgttc gataccgcct ccgtcgccgt atcgacactc tgtattgaag
    23761 cgacccccgt cagcgaacac gtgcgcatcg taataaaaac tattgcattc ctgtctcgct
    23821 gtcaatacaa aatgacaaat actatgcaaa agaaggagcg agggcatgat acacacactg
    23881 ggaatcacat cgcgcgcccg cgaggagctg atcgacatca cggacgccgt cgccgacatc
    23941 gtgaacgcgg cggacgtgga agagggcgtc tgccacatct atacgccgca taccacggcc
    24001 gggatcacca taaacgaaaa tgccgatccg agcgtgcgcc gcgacatcat cacgggactc
    24061 gcgcaccttg atctcgagcg cgtgcccttc acgcacggcg aggggaattc ccccgcgcac
    24121 gtcaagtcat ccctcatcgg gtgcgcacag ttcgtcctca tcgagcgcgg acggcttcgc
    24181 ttggggacct ggcagggcat ctacttctgc gaattcgacg ggccgcggac gcgccaggtc
    24241 ctcgtgaggc tttcgcagta agctcgagtt atcgcgccca tggtcgcgca ccacagggca
    24301 attttttctt tacaccggag gcgttccggc ctactattca ttcatgaaac gaacggcgct
    24361 caaaatactc cgaatcgcgc tcctgtgggt cccgctctcg ctcatcgcgc tcgtcatcgt
    24421 gatcttcgcg gccctctggc tcttcctcac gcccgcgcgg gtggagcgcc tcatcgtctc
    24481 gggcttcaac gagcgttcgc atggaaccat cgcgctctcc gtaaaggagt tcgacctggt
    24541 gcggggcttc cgcatcgata atcttaccat cacaaacggg cccgagttcg acaacacggc
    24601 cttcgtgcgc ctggagcggc tggtgttccg ctatcacctg ctgcgcatgc tcaccggcag
    24661 cgtccgcttt ccggagatcg ggatctatcg gccccggatc catctgacgc aaaaaaacgg
    24721 cgtgtggaac gccgcgcggt tgatgaagcc cggcgagaaa aaacccgagc ccgaaaagcc
    24781 gaaagaaccg gaaacgccgt ccaccgcgga cgcgatcacg ctcccgattt ccgtcgattt
    24841 ccttcttaaa tttgtcctgg acgacctgcg cctctccgtc acaagcccgg ccttcagctc
    24901 gaacgtcgag ggcatcaccc tgaacgtcga cgtgtgggtg cctcccttta aaaaaattcc
    24961 gaaatcgatc gatgcggttt cgcttctcga gcggctgcag atcgtcctca accccggcga
    25021 ggagatcaac ctgtcgtttt attcgaagga cgccgaggtg cggccgccgc tggtctgcgc
    25081 gctcaggctc gccttcgaca aaaagaacgg cgcccgtccg cagtttgcga gcgtcctgcg
    25141 cgtggggacg aacaaaacgc tggtgcggtt caagcgggcg catctggcgc cgctcaattt
    25201 catggtctcc tatgacctgt tctatcagcc gctcgcggac ctgctcacga tcaaccacct
    25261 ggaggtcgct tttctgaaca agaagtggct gcgcctgacc gggacggtga acagcgtgac
    25321 cgcgaagcag aacatcaaca tccgcatgac cgaaagcgtc atctcgcttg acgacctcta
    25381 tccctattat cgcagcatca ccggcgacca ccgcaccgtc ttccgcggaa cgatctcgct
    25441 gtttccgctc acggtccagg gaagcatcgc ggacctgcgc gtccgcggcg ccctttcggc
    25501 gcgcaccctc gtgttccgca acccggccgc cgaagcgacg ctcccgtcgc tcaccctcgc
    25561 ctacaccgcg caccgccgcg gcgtcgacat ggacatcacc gcttcgctcg ccatgccgca
    25621 cctcttctat acgctcaatc gcaaccaatc gggcgacaac ggcatgacgc ttacggcgac
    25681 cgtgagcgcg cagaacaatt tctcgcggat caccatcccc ggcatcgcga tgcgtttttt
    25741 cagcccgacg ctgcgccgcg acgtgctcgc gctcgatctc gcgggatcgg tcgcgctcaa
    25801 gccggcgctc tccggtcgga tttccatctc tcgtctggcc gtcgatgcgc gcccgctgcc
    25861 agtcatggtc ccgcgccatc tggtgaagac ggtcgagggc atcccgctga agaaaccggt
    25921 gaccctgggt ctggacaccg acttctcgct cggcgaggcg gccatcgccg cacggctcgc
    25981 cctgcgcggc tccgttcccg attacgacat ccccgacctc gcgctggggg ccgatatcgt
    26041 tcagcacaag gcctccaagc gggtgaccat caaccgcttc tcactgggca gcgcgcggaa
    26101 aaacctgtcg atcaccgccg ggggcttcat cgtgcttgcg gagccgccct tctccgacgc
    26161 cgacctgcgc ctaacgctct cttaccgccc gccgtcgttc acgaccgcct acggtccctg
    26221 gaagctaaaa gggagcgcgc gtgtcgacgc ggccgtcaag ggcggcttta agaagggcaa
    26281 agcgaccggc gccatcaccg tcgacaagtt cttcgtgaaa aacgacgagt cgcttctggc
    26341 cgtggacgat atcaacatca attttccgtt cgagtactac tttacgccgc gctataccgg
    26401 gcaatcccgc atcacggtgg acaaggcgca gatcatcgag aacgagtttt ttcaggaaaa
    26461 acagaacgtg acggtcgcgt cggtccgggc caagcacccg gcgcggaacg tccccatgga
    26521 atacctgaag gacctgcgcg cgacgatgtc gttcaggaac aacaccttcg agatcgcgcg
    26581 cctgcgcgcc tatgtcctcg acggcgcgct ctacggccgg aacattctgt tcaaccttgc
    26641 cgacatgaaa aagcaaaaca tggagtttaa cctggtgctc gacgtgacca acgtggacat
    26701 cggcaaactg gacgagcagg aggccgcgaa gaaaaagcgc gacgccgagc tgtcgctcaa
    26761 cgcgaacttc aagggccacg ggctcgatat cagccgggag ctctcgatgg agggcttcat
    26821 caacatccac aaaatcgggg acaagttcgc caaccggctg ctcaagggct taagcaccga
    26881 aaagggccag tcgaaactgg gggccattgg ccagttcgcc gtggacaact cgatgaatgt
    26941 gacggggttc cgcttcaacc tcgacaaggg gctcgtgtac gcgaccgtca ccttctcgcg
    27001 acgcaccttc ggctacctcc tgggcgtcaa gaacgagcgc gtcgagttcg aacgcatgcc
    27061 catccaggaa tatctcagga aagtgcagac aggagattga tatgaagcgc gcactcgccg
    27121 ccaccatctt cgtcatcatc atcgcctcga tcgctgcgac gggttgttcc tcgtctcaga
    27181 tccccagctg cctggagata tcgccccccg acatccatat gaccggcgag aaaacggtca
    27241 tcgagaagca gatcgtgggc gactacaagg agctggagaa ggacgcctgg atcatctcct
    27301 cggcgcgcac caatgtccag cggacgaagg gagcgggtcc cgccgtgacc ggcgacgagg
    27361 agctctttcg cgccatgaag atccgggagt tccaccagtc gaagctccgg acctataaaa
    27421 acgagggggc catcggcgag gcgaacaccg gcctggtggc ctacctgaac gcggctccct
    27481 acgagagcga ccgcgacctg aagcgattgt tgatgatctt catcgaggaa gagaacaagg
    27541 cgcggcgcgc catcttccgc cgctcgctgg tgaaatcggg agtcgcgacc cccgacgatg
    27601 cggcgctcgc cggtttcggc cgggcgttcg cggaggaaca gcgcgcgctt gcgaaaccga
    27661 acgactggat ccaggacgca cgcggcgcat ggaccaagaa aaagtgaggg cctgcccatg
    27721 aacggattca cgctcccgcg cggcatcatc gttgttctcg ccgctttcgt cgcggtttca
    27781 ccgcttgcct gcggaaaggc gacccgcgag gtcccgttca agtacatgga catcatcaag
    27841 accgagtaca agtacaatgt cgggaaggcc atccccgtca ccatcgacca atccatcgag
    27901 atcgacggca ccttcacggg ggacggcgcc tacttctttt tctcgtccaa ccgcgaaagc
    27961 ggcaactacg acatctacct gcgcaatctg gcggacatca ccaccgtgcg catcatccgc
    28021 cacccggcga aggacaccgc cccggcgata tcgccggacg gtcgccgcct cgccttcgtg
    28081 tcgcagcgcg aggaccccga gggcgacatc ttcgtggcgc cgatcgaccc cgccgcgatc
    28141 atcgccgcgg cgaagtcctc cgtcgacgag attccgccgc tcgatggcgc gtcgcgcaat
    28201 ctcacgcaat tccaggaccc gcagaccggc accatcaaga tcgtgcgcga cgcctccccg
    28261 gcctggtctc cggacggaac gcgcatcgcc ttttcctcca cgcgcgacgg cgccgagaac
    28321 atctggatca tggacgcgag cggcgcaaac ctccggcgga tcaccacgaa gggcggcctc
    28381 tacccctcgt tctctcgcga cggcaaacgg atcgtcttta tttcgtaccg cgatgcggga
    28441 agccggggta acatctacac ggtcgatgtc gattccggaa ccgagcggca gatcacgaag
    28501 tcgtccgcca tcaagctgca cccctgcttc gtcgccaatg accgggaggt gatctacacc
    28561 ctcatcgacc gcgacaccaa cggcgacgga cggatcgacc tggccgacaa tgccgtgatc
    28621 gcctaccaga atctggatac gggcgcggcc tacccgctca cgctgcactc gcactcgtcg
    28681 ttcatgccgc gctggttccc ggtgttcatc gacttcgaga agtcgtatcg cggcgtcgtg
    28741 ttgtactcgg aacagatcgg ccagaacatc aatatcaatg tgattcccga gaccgggatc
    28801 atcccccgat cggccaatgc ggaggagcag ttcaatctcg ccctgaaata tctcagggaa
    28861 ttcgacgact acgaacgcta cgaactcgcc ctcgagcgcg tgttccattt cttcggctcc
    28921 gggaaggatg cgcgatccgt catcttcgtg accaaggcgc tcatcgagga ggcgcgctac
    28981 gccctctcgc gcaataatgc ccgaaagagc cgcgagctcg ccgcgcgact cacatcgctg
    29041 tcctccgacg cgctcgatta ccgacgcatc gcgggggcct atctttcccg actgaacgaa
    29101 cgccgtccgg gcgaggagac gctgcgcggc gcgatcgccc gcctcagtgc gcgcgatccg
    29161 aaatcggaat tcatccccta tgtcatggag gacctggccg ccgagctgat gcgcgtcggg
    29221 aacatggccg cggccgaatc gacgcttcgg gcaatcgccg ccgagcatcc gaagtaccgt
    29281 cgcgcggccg ccatcaacta tacgctggga agcatcctct tccagtccgc gctcgacccg
    29341 ctgccccagc cgctcatcca ggtcctcgac accggcagca gcttccagaa gatcgacacc
    29401 gccctcctgc tcatcaaacg ctaccaggcc gtgcgtgatc cgcagcgacg catcgacgcc
    29461 gcccgacggg cgatcgagac gcatcccggc aaaaaatccc tcgccgggac gatgctgtac
    29521 gtcatcggcc tgtcccgcat cgagcaggga aaactcgcgg aggcgcggac ggcgctcagc
    29581 gaggccctcg cgcaggtgtc caagttcgat ctgattttct tccgggcgaa cgtcgcactc
    29641 ggcggcattg cggagcggca gggcaatgcg aacgatgcgg agcgcttcta ctcggcggcc
    29701 gcgaacaact atctcctgtc ctggaaccag ccggacatcc gcacggttat ccggaagctc
    29761 atcgatcact acgaggaaca cggcgagcgt ctcgagcgag cgggctcatg gaacgcggcg
    29821 gtcgacctgt acaagaagta cgtatggctg gtcacctata tccacctgaa caagcgcttc
    29881 gaggacctct acaatgaata cgcgccccga gcgcacgtcc tgtacatcga cgcgcatgcc
    29941 gcctggaagg gtaattccgc tgagtcgctg tcggcgcttg aaaaagaata cctggcacgg
    30001 ctgcccatcg cgcgcaatga cttcgacaag gcgcacatct acggcctcgg gtatatctac
    30061 gcccggagcg ccatcaccaa cgacgcccgc gcgtcctcgg tcctgcccgg catcgcgtcc
    30121 atcaccggga cgcgcctgcc cgatcttttg gccggcctca agcgcgccgt cgaccagatc
    30181 gaatgggccc tcttcatcga cgacaccttc gtcgacgcct acatactcaa tggctggata
    30241 ttccaatacg tggacctgct gcgccgagag gacgccgcga cggccggagg gaagaacgag
    30301 cgcgtcttcg cgcgcttctt cccgaagtac ctctgggagc gctccatccc cctgtacgag
    30361 aaggcgctga cgacaaatga cgagaccgcg aaccccgagg cggagggcaa cctgcacctc
    30421 aacatcgcca actgctattt tctgctcaac aactatccca aggcgctcac gcattacgcg
    30481 ctcaccaacc gcttcaagaa ccggttcaat tcgccggtgg aggaggccct gttccgcttc
    30541 cattacgcct actgcctgtg gcaaaacggc gatatcgcga acgcgcgcga ggagatgaac
    30601 tcgacgctgt cgatctatcg acgattggcc tccgggaaga atcaacgcgc ctataaacac
    30661 cagattctgt acctgtaccg atacttcgcg ctcttcgacc gcatggaggg caagcacgcg
    30721 agcgcgatcg actggtacca gcgcatcatc gatttctcgg cggagaacgg tatcgagatc
    30781 gaccgcgcgc gctacctgca ggagatcggc cactgctacg gcgagctggg cgatacggac
    30841 cgcgcgcagc gctttctcga gcaaagccag gtcatcctcg tcgatgcgcc ggaccgccgc
    30901 cagacgcaca agatcaagtg gcgcgtcttc ggcatatcgt ttctggtggg ccagttcgag
    30961 tttttcgacc tcggtcccga cgtcgcggtc atcggcgaca accgactcta ctccgagctg
    31021 gacacctaca acaagaagct cctgaacctg tcgctcttcg agtcgtacgc gaccgggcgc
    31081 gccgaccacg ccgccgccat cggacgcctc acggagaaac tcgcgctgct caagaagcgc
    31141 gactaccgcg tcaaccgcga agccatcgtc cgcacgctca acaatatcgg ctatcaccac
    31201 ttcatgctgc gcgattacgc cgcgtcggcc gagcactttc gcgccgcctg gcgctccgcc
    31261 gccgatccga agataaacga cctcgacggc atgttccgcg ccgtgatgaa catggtaaac
    31321 ctctacgcct atcgcatcga gaacggacgc attgccgact ccgcggcgcg caccgagctc
    31381 gatgagctgg cgcgcgatat cgcctcctat cgagccgcat acgagacggg tcgcctcgag
    31441 accggcctca aacaactgga ggcggccgcg aaggcgcgcc gcgaggccgt gaccgagcgc
    31501 cagcgcgacg ccctgcgcga aaagatttca accgatgccg ccgccatcta cttttccctg
    31561 gacatcgccc agggcatcgt gaccttctac cgcgccgagc tgcgctatcg agccgaattc
    31621 gcgggtaagg aaccgcccgc cgcattcgat atctaccgcc acaaccgcga actttacgac
    31681 ctgtatgccg atgcgcgttc cgcgttcgac gccgcagtcg ccaacacgtc gcgggtgcgc
    31741 tcgcgggaat tcgcggtgaa gctcctgctc aatcgcgcga tctgccagac gcgcctcgca
    31801 cagtacgacg aggccgccga ttcgtaccgc gaggccgaga cgatcgcgga gaaattccgc
    31861 ctgcgctcca ttctctggga ggcgcgcttc caagcagcgg tctttctggc acggcatggc
    31921 cagcagaccg gcgagcgcaa tcatatcgcc cgcgccgagg actattttac ccgcgccgcc
    31981 gagcagctcg aacggccgcc ctatcgcgcg tcggcgaacc tcgaccgcgt ccgagcgggg
    32041 atcatcgccc acgcgcgctt ccttgcccgc cgcgggctgc atgagcgcgc ctttgccgcc
    32101 accgaacggc tcgcgcgcat tgttcgcgcc caccgcttcg ccgcgtcgtc cctttcattt
    32161 ccttctccga aggacgccgc ggccgtttcc gcgtatacaa aactgcatgc gtcgatagaa
    32221 tccgacgagc acgcactcac cgccttcctg gagggtggcg gcgacatcaa agccgatcgc
    32281 gcacgcgccc tcgcctcttc gatctccgaa catgagcgcg cactcgctga cctgcgctct
    32341 cgtctcgatc gggccaaccc gcatctctca tcgtgcctgt tcgtgcagcc ggttccagtg
    32401 cgcgcgccgg ccggttcggt cgtctacgca ttcctcgaca atgacgatac gctgatcgcc
    32461 tggacgctcg caggcgccgc ccttcgtcat gcccaggtgc cgcttcccca gggacaatcc
    32521 tcgatctccg atgccgttcg ctcgcacatc acgggcgata cggcgaatgc gcactcgcgc
    32581 cgtttcatca tcctttccga tacgtcactc gcggcatacg ccgggcgtac cgcacccgaa
    32641 atgcccgccg caatgctgat ccccgcgctc gaccgcgtct cgcacgcgct cgccgctgac
    32701 aatgttccca tatcgcgcgg gctggtcatc ggcgtcgacg ccgcgcgcgt catcaagggc
    32761 cgcaccgcat tctccgccct gctgtttacc gcgctggact cggcagccgg cgatctcccc
    32821 cactcccaac ttatttttga tgataccgcc gacggccttt cgccggcgtc gatcatttcg
    32881 cgcggcctac gaccccgtgt cctcatcaag cgccttcggt ccacgaacta tacggatgac
    32941 ctcctatttt tcagcgaggc ggctctctat gccggcgtcc gctcgctcgt tgtctatacc
    33001 gggctttcta ccgccgaggc ggccgatctc gtcgaggcga cgctgtcgag accggcatcc
    33061 gagatcgcct cgaccgccgc gcgacgcggt gccatcctag ccttcggcaa ccccgacaag
    33121 ccgggtcagg cgggcgccga tccgcagaaa agcgccaccg acgccttttc gcgttatacg
    33181 cgcttcctcg aatcgcgccg cttcgatgat gccgcacggt atctcaccat ctggcgcgac
    33241 accgccgcgc gcgacgccgc gacgaacgcg cgcttccatc tgctcatggc cgatctcgcc
    33301 taccggcgcg gcgaccttgc cgccttcggc gcgtcgatcg gacaggcccg ctccctcacg
    33361 ccgataaaca cgtccgatca tgcgcgcgcc ctcggcggcg cattcctcca ggaatgcctg
    33421 agcggcaatg ccgtgacggc gcgaactatt ttcgatgaat atcgcgcgcg tacgaatcgt
    33481 cccgcgggcg atggcatgga gatcatgcag gcgctgtgcg cgctcatgga gcatgcgccg
    33541 gaacgttctc ttgccgcagt gcgcgggctt tccgccgccc ggatcaccga gccccacgcc
    33601 gtccgcctgt tgcccgttct cctccgtttc gccgaactct acggccatcg acagctgcgc
    33661 gcttcgctga ttgcgaccat gcgaagcgag ccgctccgct ccgcgctcgc ctcgtcgata
    33721 tcggacatcg acgcgatccg catggccgcc attcccgatg ccgacggcat cgttccgctt
    33781 cccgatgatg ttcgctggct ctccagtgcg actgacgcgg ccacgctccg tgcgcgcgcc
    33841 ttcgaggcca ccgccgccac gaccgcttat accgtccgta ctgcgccgct cctgtccgcg
    33901 tcgattcgcg cgctcctggc gcgatatcgc ttcgacgagg tatccgcact catcggtcgt
    33961 atccgcaccg gcgacatgat cgccgcgtca cattggatgg acgcgctcgc gctgcttgac
    34021 acgatccgcg ccatccatac cgcctatgaa cgaagagacg aagcgatcgc gaccgtcacc
    34081 gcatcgacca cgctcctcga gcggatcccg attgcctcgc tcctcgccga tcgactctac
    34141 gacgaggcgg tcgcccatct ggcgagatcg gaccatgccc gagcgtccgc gtccgtgaat
    34201 cgcgcgcttg cgataacccc ggacggcgcc gacctgaaga cgcggctgcg cttgctcgag
    34261 atcgagctca tgataaccca gggatcggtt gacgccgcga acacgcgcct ggccgcgatt
    34321 gagtcgacgc tcccagacga ctatcgctat gatcgctcaa tgctgcgatc gctcatcgtc
    34381 cgccacggca tcatccaggc ggccggccag cgtcagagcg ccgttaccga actcctgcag
    34441 acgtacgatc gtcttatcgc cgacgccatg gacgcgctcg acgccacgcc cggacgcgtt
    34501 cgcaccgtgc gccgcctcgc cctcatcgaa cagggccttg atttttcgat cagctatcgc
    34561 ctcagcgccc gtcggctcgt cgattcgctc tcgctgttcg aagcaaagac gcagatatcc
    34621 ttccgcgcac gcatgagcgg cgagcttgat tcccgcgccg tcccggcacg ggcgcgcggc
    34681 tccttccgcg cactcacggc gctccaggcg ggggaccccg aagacgttcg cgcgctgctc
    34741 accgagtatc cgcagctcat ggtcctctcc tccgtgcgcc atgtcccgat cgccgcgttc
    34801 caggcatcgc ttccggccga cgccgttgcc atcgtcgtcg cgcgcagcga ggccgatctc
    34861 tacatctgga cgctcgaccg gagcgacatc aacgcattcc gcatccgcaa cggcttcgta
    34921 cgcatgacaa agctcatcgc ggattatcac gaactgattc gagaacggcg acaccccgca
    34981 gtgctctcca cggcgcttgc tgcggtcttt gagccggtcg aaaagcactg gcgcggacgc
    35041 cgcaccatac tgttcatcac cgaccgcaac accgaagcga ttccctacga actcatcgga
    35101 cgcgaatcca tccttgatga aacccatgcg atcgccttcg ccccctctat ccttgcggcg
    35161 ctgcgcgagt atccgtcggt gcgtccaacg gtgctcctcg gcagcgccgg cgcgccgtcc
    35221 ctcgccgcga acctcgatcg gatcgccata cgagaatcgg gcattaccgt cgattcgggc
    35281 tcccgatcaa cgggcttcac gcatgtcgag gggctggtcg cctatcaccc gctcgcgggg
    35341 gccgtctatg tcgattcggt tccgtaccgg cgcgcgctca acgcatcgcc gggcgcctat
    35401 cttgcctcgc tctcgccgct cgagcgccag gccggaaacg atctcgctct cctgggggcg
    35461 atcggcggat catccgtgac ggtcatcacc gccgccgagg cgcgcgacgt caatgcggcg
    35521 ctgttcaccg acgcctttta ccgtgagttc tcgcggagcc gggacctcgt gcgcgcatcg
    35581 tcgcgcggcc gggccgcctt gcgcgccgac gaacgctatc ggcacccggc ctactggtgc
    35641 ggcatacggc tctacctgag ctcgaaccga catctgtccc gcggcgtgaa atgatgcttg
    35701 caattcaagc ggcatgatgc cacgctacgg catcagccgg tcgatcccat gagcgatact
    35761 cgtcatccac aacggaccat cgcgatcgtc gacgacgacg acgcagtcaa ccaggtcctc
    35821 acgcgtatcc tgcagcgcga gtacgcggtg cgcaacttcc tgtgcgccga ggacatgatc
    35881 gctgagaacg cggccgcgtc cgtggacgcg gtcatcaccg acgtggacct cccgggcatc
    35941 tccggcatcg agttcctttc gaagctgtgc gcggacgatc cgaacctgcc ggtcatcgtg
    36001 atcaccggct acaatgatat tgacatcgcc atctcggccc tcaaatcggg cgcgttcgat
    36061 ttcatcctca agcccttcaa taacgaccag atcatccttt ccgtccgccg ggcgatcgag
    36121 cgccgacgcc tgttgatgga aaacgccatg ctcatcgaga cactcacggc gaaaaacgcg
    36181 gagctcgagg agctcaacgc gaggatcagg gcccgcaatg tcgagatcga aaacgagctc
    36241 gatatcgcca gcaatcttca gcgctgcctc ttccccgcgt cgctccccgc catcccggga
    36301 ctcgagatgg cgctgcagta ccatccggtg gagaaggtga gcggcgattt cttcgagttc
    36361 gaccacaccg acgaccgccg cttctcgctc ctgctcgccg acgtctctgg gcacggcctg
    36421 ccggcggcgc tgtattcggc gatgatcaag gcgggcaccg acaccatcaa cacccggtcc
    36481 gccctgccgt ccgagttcat gcgcgcgatg aacaccttcc tcatccggac ccagaagaag
    36541 atgagctaca gcttcgcgac gctcttcttc ggccgcttcg atctcgcgga caacaccatc
    36601 acctacacca acggcggcat cccggcgccc atcctgatta aaagaggcgg cgaggtgatg
    36661 gcgctcgatc cgaacgcgtc cttcatcggg attttcgatt cctctcgcta ccgcgacgat
    36721 gtcgtgccct tcgagccggg cgacacgctg ctcctgtaca ccgacggcgc cttcgagata
    36781 cagaacccgg agcacgacat cctcgggcac aacaccttca tcgagatcgt cctttcccac
    36841 cgggaccggc cgatcactga gatcgtggac aacaccttca acgacatacg gtcgtttttt
    36901 ccgaacggat cgatcatcga cgacatcacg atcctgggca tgcgccggac gggctgatcc
    36961 gggaaatcgt ttgacagcag acaccattcg cgcgaacacg cgcgaaatat aaatcatcgg
    37021 gaggacagtc atgaaatcga tacgccacgc catgcccgtg cgcatgtgcg ccgccgcgct
    37081 catcgcggta tcattgttcg cgcccgcaac gctgcacgcg ctcgagatca ccttcatggt
    37141 gggctcggtc tcgctcacgc gcggcgggaa gacgtccgca ccggccgttc gcacccggct
    37201 ggcatcgggc gataccctga cgacgggcaa gggctccctg gccgacctgg ccctcgccga
    37261 cggcagccag atcaaggtga tggagaacag ttccatccgc ttctccggcg acatcaagga
    37321 ccgccccgat tcggtgtccg tgatcgcggg catcgtcgag agcaagttca agaagctcgc
    37381 gaagggctcg cagagccgta tcatcacccc caccacggtc tgctccgtgc gcggcaccga
    37441 attccgcgtc gcggtgagcg acagcggcga ttcgcgcatc gacatgcagg aaggcgcggt
    37501 tgaactgcgg aacccgtatg ggaaaaccgc gatccgcaat cgccagcagt cgtcggtccg
    37561 catgaacgac aagccggcgg ccgcacgtga aggcgcggga ctcgagcaat ggaaggagca
    37621 gcgcgaccgc gccctcgatg aaaatccccg cgaacaaggc ggccggatca aaacatatct
    37681 gggaaccatg aacgatcgct ccgcgaagac atcggaccgc atcagcggac tcgggtccga
    37741 ccttcgcgac aatgcccccc ggacgaagcg ggcgcttaaa aaatccggca ctgaactcga
    37801 tcgcctcgat gaagaggtga aagacgacct gtatctgagc gaggcgacca atgaatcgct
    37861 ggggcggatg tccgagcgct tctcgaaggg ccagggcgat atccgcgagc tgttcacacg
    37921 cctcaaggaa gagtcggacc gcgtctgcga gcaaaaacgg aagaactacg aaagcatcca
    37981 ggcggtcaag gccgcctatc gaaaggcgta cgacgagatc atgggcacca tgaacacgga
    38041 gcgcgagaag attcgtcggc agttcgacga gcagcgtgaa accaaacccg agtgaggcgt
    38101 cggtgatgcg cgtgtatctc gttcaacacg ccgatgcgct tcccgccgat gcggaccccg
    38161 cgcggccgct ctcaccaacg ggcgtccgtc aggcggacgg cgccgcgcgc tttctcgcgt
    38221 cgctctccgt tcgtcccgac cgcatcgttc acagcggcaa ggaacgcgcc cgccacaccg
    38281 cggagatcat cgcaaccgcg ctcgggtgcg cgtccgtcga accgcgcgcc ggcatcgatc
    38341 ccaacgacga tcccgcgcca atcgccgagg agctcatcgc cgcgacggat tccccgatga
    38401 tcgtcggcca catgcccttt ttccgccggc tcgcgaacgc acttctcggg accggcgcac
    38461 tcgaccccat cgacttcgac aatgcctcgc cgctcgtgct gatccgcgac ggcggccgct
    38521 tccgcatcga ggcctacgcg aaaaacgcca tcatcgcctg aaaagggatt gtcgatttgg
    38581 ccccagcggc gttagctgtc tgcaatggac ggacgcgcac ggtcgccgta tcgtgcaaac
    38641 aatcattccg aggtgccgta tgaaaacacg cattaccgaa ctttttaaaa tcaaataccc
    38701 cattctcctg tcgggcatga gctggatcag cacgcccgag ctcgtggccg cggtcgcgaa
    38761 tgccggcggc ctggggatcc tcgccaccgg tattttgcag cccgacgaga cgcgcgatta
    38821 catcaagcgc acccgcgagc tcaccaacaa gcccttcgcc gccaacgtga ccctctactt
    38881 ccccggcgcg gaccggaacg cagagatcct catcgaaaac aaggtcccgg tcgtcaacta
    38941 cgcgctgggc aagggcgata aaatcgccaa agccgtccac gcctacggcg gcaaggtgat
    39001 cgcgacggtc accacgctca agcacgcgat ggccgcgcag cgcgacggcg ccgacgccct
    39061 catcgtgacc ggccacgagg ccgcgggcca cggcggcgcg gtcacctcgc tcgtgctcat
    39121 cccgagcatc gtggacgcgg tggacatacc cgtgatcgcc gccggcggct tcgccgacgg
    39181 acggggcctc gccgccgcgc tcctgctcgg cgccgagggg atctccatgg gcacgcgctt
    39241 catgaacacc acggaaagcc cggtgcacga gtcccagaag gcgctcagca atacgcttac
    39301 cgtgtacgac accgtctaca ccgacaaggt ggacgggctc ccggcgcgcg tgatggcctc
    39361 caagggcgcc acccggctca tcaacaagcg cctcaatccc ttgagcgcgc tcatcaactc
    39421 ccgcaagatc gccaagctcc tcggcttccc gtggctcaag ctcgccgtgg gcatcatgct
    39481 ttccggagtg cagaattcca tccgcatggc ccgcatggcc atcggcttcg acgccttcaa
    39541 ggccggcacc atggacggcg acaacgagaa cggggtgctc cccatcggcc aggtcacggg
    39601 gatcatgcac gatacgccgt ccgtcaagac ggtcatggac cgcatcgtca aggaagcggc
    39661 cgaggccggg aaaaagctcg ggaaaaagct gtaggccgat cccggtcgcc atgcgcccgg
    39721 cgctcgaaga gcttgcccca cggatccgga ccgcgctcgc aacggccggc gtcgagtacg
    39781 ccgaggtccg gatctccgaa gggtcaatga ccgagatcac cctctcgggc gagcaggtcg
    39841 actccctctc gaccggtgat ttcgcgggcg gctccgtccg cgtccgctcg ggcggtggat
    39901 ggggctttgc gtcgttcaat agcatcggcg acatcgagtc gtttatccgt cgcgccgccg
    39961 ccatcgtact tccctccgtc ggcggcgcgg cgccgggcat acgacgcacc ccggccatat
    40021 cggaccgcgt cgcgatccaa tcgcgtatcc ccttcaccga cgttccgctc gacgccaagt
    40081 acgagctcat cgcgggctac aatgcgattc tccgcggctc ggccagcata cagacgacgc
    40141 gcgccaccta ccgcgacgtg gcggcgcgtt cgctgttcat caacaccgac ggcagcgagc
    40201 tctactacga caggtcgtat tgcggcattg cgctcgccgc gatcgcccga gacggatcga
    40261 acatccagac cgccggcgaa tcggtgtccg gatacggcgg gtacgagatc gcgcaacagc
    40321 gcgagtctat cgctgagcgc gccgtgaaga cggccgtcga tctcctcggc gcggagcgcg
    40381 tgggcggcgg tacgtacgat gtgatcaccg accagaagct ctccggcgtg ttcattcacg
    40441 aggccttcgg ccacctctcc gaggccgatt tcatccacga aaacccgcgc atgcgcgaga
    40501 tcatgaccct cgggacgcgc ttcggagttc ccgagctcaa cgtgatcgac gacggcaccg
    40561 tgccgagtct ttcgggctac gttccgtacg atgacgaggg cgtcccctcg cgaaaaaccc
    40621 atctcatccg tgacggcatc ctcgccggcc ggctccactc ccgcgagacc gccgccgcca
    40681 tggacgagga gctcaccggg aacgcgcgcg cgatctcgcc gatggcgcag cccatcgtcc
    40741 gcatgaccaa cacctatatc gacaatggcc ccgccgaaac cggggagctc ttcgacgccg
    40801 tcgggaacgg catctacgcc tgcgacgtca tcggcggaca gaccaatctc gagatgttca
    40861 ccttctcggc gggccacgga tacgagatca aaaacgggaa gcgggggaag atgctgcgcg
    40921 atatcgtcct gtccggaaac gtctttacga cgctcaacgc catcgccatg atcgcgaacg
    40981 atcgcgccat gttcggcggg ttgggcgggt gcggcaagca gggccagggc cccctgccgg
    41041 tgtcattcgg cggaccgcac atgctcatga aaaacatact catcggcggc gtgcagcgat
    41101 gatcgaagca atagataaac gcctgcgcgg cgcctgccgc tggtacgact gcctctgcac
    41161 gcaatccgaa tccactgtcg tctcgttcag ggacaaccgc ctgcatgccg tccgcgagag
    41221 ccagaacagc ggatgcgggc tccgcgtgaa tatcgacaac cgcgtcgggt tttcattcac
    41281 aaacgatgcg tctacgccgg acgggctcgt cgatcgcgcc cgcgccaccg ccccatacgg
    41341 cgacatcgac gattacgatc tccccggtcg tgcaacgctc ccggcgacag aaccataccg
    41401 tccgtcgatc gattcctttt cggtcgagcg cgagatcgat gcggcccggg cgctcatcgc
    41461 cgagcttcgc ggccgctttc ccgatctgtc ggtcgatctc acggcatcgc gcgccacggg
    41521 caccaggcgt cttgcgaact ctaccggctt cgacgcatcg taccggcact catcgtacgg
    41581 aatatccatt cacgcgaact atgttttcga ttcgggcgcg cgtcttctcg tctgggaggg
    41641 gacctccgag ctcggacccg tcgactatcg acacctcctg gaccgactga tcacaaagct
    41701 cgacaatgcc atgacggtcc gtaccgcccc cagcggaaag ctcccggttc tggcaagtcc
    41761 gcgcgcattc gcaagtcttc tcgggattct caaatcgggc ctctcggcaa aggcggtcta
    41821 caagggggtc tcgcccttcc agaacaaact cggcgagcgg gtattccatg aggctttctc
    41881 ccttcgcgac aatccccttg ccgaggactc tcccgccggc tatcccttcg acgacgaggg
    41941 aacggcggcc gccgacaaga tcatcatcga tcgcggcgtg attgccgggt tcgtgagcga
    42001 cctgaagcat gccgcgcgcc tagggcatgc gcccaccggg aacgcctcgc ggggatatgc
    42061 ctccctgccc gttccgtcat ttagcgcggt gaatgttccg gggggcgtga ccgcgagcgt
    42121 cgggctcatc gaggggatgg atcgcggcgt tctggtggag caattcatcg gcctgggaca
    42181 gtccaacacc ctcacgggcg atttttccgc aaacctcgat ctcgcctatc tcgtcgaggg
    42241 cgggcgcatc gtcgggcgcg tcaaggactg catgatatcc gacaacctct tttcgctcct
    42301 cgccgcgcca ttcgagctca gtcgcgacat cgagcgcgtc ggttccgcga cgctcccgtt
    42361 tgcgttcttt cccgcggtca attacaccgc gaactgaagc cgtcatttcc gcgcccggca
    42421 gtccaggaac atcggatcaa agatggtgcc gatgagcaac cgcccgtctg tcgcggcccc
    42481 gacgctcgat gcggagatgc cggtcccggg atcaacatat acatcagtaa tgcgaaagtc
    42541 gccgtccggc ccgaaccgga ccgtgagcac ctgtgacggc gacaggagcg acggattgtc
    42601 ccggtgcggc agcaccttca tgacttgtgg atgcgccgcg atccagagcg ctcccgtatc
    42661 gtccaccgag atattgtccg ggcaggtccc gagatcgatt gacgcgctgt ggtcgagcgt
    42721 gccctgcgcc gggtcgcggt cgtagacctc gagcgagcgt cccagcatcg atgccacgaa
    42781 cagccgcttc ctgtcgggcg agagcgcgat gccgttggcg aacacgagcc gacgcgccgc
    42841 cgtcgtgaac cgcgcgccgt cgaagtgcac caccgatccc agggggagct tgagatagtt
    42901 ttcgacggtc cgccccaggg gcgaggtgag gccgcaatcg ttggtcacat aaaacgagcg
    42961 cggacccgcg gcggcgatat cgttgggact ggtgatgagg tcgctgtgca gcgattcaac
    43021 gtgggtgagc gcaccgtcgc gatagtcgaa gatctcgata tacttcccgt cggcgcgaat
    43081 gttgaccgcg aacagccgca cagtctcgcc cgaccggtac aggtcgattc ccagcgggtg
    43141 gaactcgaag gcgagcgcgg gggtgatgtt ctccgggacc gcatgctcga ctgtcagccg
    43201 atagcggtac accgcccccg ggacgcgctt tccacgcagc gccgcgcgtc gatcgtcgga
    43261 agagatgaag gcgatcccgg cggcgcgatc gatgacgatg tcctccgggc ccggaatgtc
    43321 ggtcacccgc gagcaggcgt atggggcgtt cttcatgggc tcgatggtct tgaacacgcc
    43381 cgcttggtga agcaatttca ccgtaaaaat aacggcaatc acaagcatga tgacgatcca
    43441 ggtaagaatt tttttcccgg tcatgatacc ctccctacgg tttgcgcggc gcattccatc
    43501 atgtcctacc atggtcaccg ccacctgtca agcatcgcgg cggtttgtct ctctgataaa
    43561 ctcttgactt tccgatcgcc gggaacaatc gtcatcaccg gacggaaacg acgcatactg
    43621 ctgggaggcg ccatgatcac ctatgccggc ttcgatgagc tcatcgcgcg cgtctatgcg
    43681 caccgacagg accatgtgtt cgaatactgg ggcgagctca tcgatgcgga gcgacgcgag
    43741 cttctgaacg atctcaccgg gatcgatttc gaaggattgc aatcgctctt cgcccaaacc
    43801 cggcgctcgg acgccgtcgc cggcgagttc ggccccgcgc ccttcattcc catcccgaaa
    43861 accgacggcg agaacgcaca gcgcgcgcag gcccgtgagg ccggcatcgc gcatattcgc
    43921 gcgcgcaagg tcgcggcctt cctggtcgcc ggcggccagg gaacccggct cggctacgac
    43981 ggtccgaagg ggaagttccc ggtgagcccc gtcgcgggca agaccctctt tcaaatccat
    44041 gccgagaagg tgctcgcctc cagccgggcc tatggcgtgg acatcccctg gctcatcatg
    44101 acctcgatcg cgaaccacga ggaaaccgaa accttcctcg aggagcacga ttacttcggc
    44161 atccgcgaaa cgctcgttca cgtctttccg caaaacctca tcccctcgct cgatgcaaac
    44221 gggagcctga ttctggaatc gaaaaaccgc atttttaaaa acccggacgg gcacggcgga
    44281 agcctcaccg cgctcaaaac gtcgggcgcg ctcgacttcc tcgccgaaca tggcatcgat
    44341 acgctgtcct actttcaagt ggacaatccg ctggtcaggg tcatcgaccc cgacttcatc
    44401 ggctaccacg agctcaacac cgcggacgta tccagcaagg ccgtcaccaa ggccggcccc
    44461 gaggagaagg tgggggtgtt cgtacgcttc ccgaacggcg cctgcggcgt cgtcgagtat
    44521 tccgacatga cccatgaaca acagcacctg caggactgta ccggcgcgct ggtctattcg
    44581 atggggaata tcgccatcca tctgttctct gtcgagtaca tccggaggct cacggcgggc
    44641 ggcgcgatcg agctccccta ccatgtcgcc cgaaaaaaga tcaaatccct gcgcgccggc
    44701 gcacccgccg agatcgacgg cttcaagttc gagaagttcg tcttcgacgc gatcccgctc
    44761 gcggagcgct ccatcgtctt cgagaccggc cgcgccgagg agttcgcgcc ggtcaaaaac
    44821 cgcaccggcg tcgattccgt cgataccgcg cgcgagatga tgagcgccct gttccggtcg
    44881 tggcttgccg cgcgcggcgt tccggtgcct cccggcgtcc gtgccatcga aatctccccg
    44941 ctcctggcgc tcggcccgga tgaccttccg gcggacctca ccgtccccga ccggcccgac
    45001 gtctatcttt cgtgacgcgt tccgttctct tcatccagct tccgctgctc gaccacggtc
    45061 gcttctatat cgccggcaac gtggactacg ccccggcgtc gctcacggca tatctccacc
    45121 gacggtttcg gaacaccgtc cgctgcgagt ccctgcccga tacgctcgcg aatttcgcat
    45181 cgaaccggtt gatcgcgcgc tacatcgacg cggtcgatcc ggagatcgtc gccttcacct
    45241 gctacctgtg gaacatcgag cgcaatctcg agatcgcgcg ggagatacgc gagcgcgcac
    45301 cgcgccggac catactcttc ggcgggccgg agatcgctca gggctcgtgg gcgcttgccg
    45361 agccgcggga tgcagtggac gccttcgtga ccggtgaggg cgagtggttt ttctcccgtc
    45421 tgctcggcgg cgtcgatctc ggaccatacc gcaccgacat ccgcggaaac gcggtatacg
    45481 cccagccggc ggccgagctc ctgagcgccg atgagatcgt cgagccgttc accgccggct
    45541 atctgaatac gatgaacgac ggctccatct tcctcgagct ggtgcgcggg tgtccctatc
    45601 gctgcgccta ctgcaattat tcgaaaaaca gcgcgacggt ccgcgagctt cccttcgacc
    45661 ggctcgtcac ggcgatcgag cggggacgcg cgcacgagct caccgagatc tacatcctgg
    45721 cgcccacctt caaccgctcg tccgatttcg acccgctgct ccggcgactc gcggcgatgc
    45781 ggcacggcgt ccgcctgcac acggagatgc gcaccgacgg catcgacacc gacatggccg
    45841 cccgcatgta cgatgccggg ttccgcagcc tcgaggtggg gctgcagacg ctgagcacac
    45901 gcgccctcag ggagatcggc cgatcgtcgc gcaccgacgc cgagctcgcg ggaatgaccg
    45961 cactgcgcga cgccggcatc gatctcaaga tcggcattat ccccggtctt ccgggcgaca
    46021 cgccggaggg attcatcgga accgtcaccc gcctcatcga ggagggtttc ggcgaccaga
    46081 tcgagctcta tccgctgctc gtactgccgg gaacccgcat gcgcgacatc gcctccaatc
    46141 ggagcgcccg cttccagtcc gctccgccgt actatttcac tgacggttgg ggattcgacg
    46201 agggtatgat ccgcgacatc gtcgcgcgcg tccgcgccga aaccggttat gaatacgaca
    46261 ccatccgcat accggactgc ctggccggtg ccgggagcgg catcgtcggc ggcgtccact
    46321 tccgcggcga tgatcccgat cgctgggacg gcgcacgcta ccgcgatatc atcgacacca
    46381 atgtcttcac gatcaccgtc tcgctgggct cgcctgatgc gatcgtccct ggcctgcccc
    46441 ggctcttttc gctgctgccg gccgatgttc tgtacacgct tatcttcgtc tgcgatcttc
    46501 ccatccccga acagccgatc gtcgattttc tcacccggcg ggagtcggac agttttctga
    46561 tgcgcctgcg catcttcgac gagccgaaac gggacagccg cattcaatgc ttccaggtca
    46621 ttaccgatcc ggcggtctat cgaattgcgc acgatacgta ccgcgtcatc gaacccatat
    46681 ttcgattgac catggacact atggatgcca taccgctggt gaggcgctgc gagcggctgg
    46741 gcatggttct cgcccccggc gtatacgaac atgttgcacg gtttatcatt acagaattca
    46801 gcgaccgccc cgaccggatc ggctttgaat ccgaaaccga catggcccga ttctatcgcg
    46861 agtgcgggct cgatctcgtt cgcgttcccc ttaccccgtc tgttctggac cgttaggccg
    46921 cgacattcag attatgtcac attggctttc gccatttcca ttcgacttat cgtattcctc
    46981 caatcgctca ttgtatggat cgttcgggaa ctttcaagag tcttcgcggt ctcatccggt
    47041 agcgggcgca gatcacgcgc agcggacggt ccgtccgacg aggagctggt ccgcgcgttc
    47101 acgaacgaca cggggcgtcc gcaggagcgc aatcgcgcct tcgacgcgct ggtgcgtcgt
    47161 catcagcatc tcgtcttttc gctgtgcgcc cgcatcctgg gcgactacga cgaggcgagc
    47221 gattgcgcgc aggaggtttt cataaaggtc catcacaatc tgggtgcgtt taaaatggaa
    47281 gcgagcctca caacctggct gtatcgaatc gccgtgaaca cctgccggaa ccgcatggcc
    47341 agcgcccagt accgcgcgcg gcagcggagc gtcaacctgg acgcgggcga tgacgacagc
    47401 gccgacggcc caccccgcga gctgggcgac gacagctttg cgcccgagaa cctcgtcatg
    47461 cgtcgggagg acgcgcgcgc catccagcgc gccatcaaca ccctgccgaa ggcgcagcgc
    47521 ctgctcgtcg tgctccgaga cgtcgagggg aaaagctatt ccgacatctc ggccatcacc
    47581 ggcatcaagg aggggacggt caagtccggg cttgcccgtg cgcgcgaacg actgcgcgaa
    47641 atcctccggg aggcgttcga tgacgtgtaa gggaacacgc cgcacactct ccgcctatat
    47701 cgatggagtg gttgatccgc gaacgcgcgc agcaattgat gcgcatatcg ccgcctgccc
    47761 gcgctgcgcc gacgagctcg cccggctcac ccgctatcgc gcgcgcatgg ccgcccttcc
    47821 ccgtctatcg gcccccgagg gcttcgccga aaccgtacgt actcgaatcg cggctggcgc
    47881 cctgcctcag ccggaaacca cctcgatcgt tacgaaaatc gtccgtaccg tcacgtctgc
    47941 gccgcttcgc attccgatta cggcggtcgg tgcgttctct gttgtcatta tcatcgcgat
    48001 ttttttccaa cgatcatccg acattgcacc gccaccgtta acagaaaaac gatacttcgg
    48061 tatgcaacag caatcgcagg atactaccga tttggcccgc atgcgcgatg acgccgcccc
    48121 ctccgatacc gcaaccgatg cgttggaacc catgcggagc aaatcggact caccgcgatt
    48181 tgggcgaaaa cagaaaaccg cgaaaccggg gatcgtcgcc gatcgtccca tcgagatact
    48241 cctggcgatc gccgatacgc ggtccatgcc ggccgatgat gagatcggtg cgggcgacat
    48301 ggccacgatg cgcgaggcgg aggagaaagc gcccgaagcc ccagcggcgc tgaagagcga
    48361 gtcacgtgac acgaagatgg cgctgtcccg gaagcgggcg gcggacggca tggctcgcgg
    48421 ttcatcgatc gaacggagca aggacgcccc ggcccgaccg gcgctgcggg attccggcac
    48481 cgccatcagg tccatcacgt ccgatgtgcg gggccgcgtg attgcggaag agcgcgatcc
    48541 ggacacccgc atgctgcggg cgatcaccat cgatgttccg gcggcgaagt atgggacatt
    48601 cgtggaacga cttgcgggag tcggtagaat cagcatgccg ccaaagcgtg aggggccgca
    48661 cggccgtgac tgggtccgca ttcgaattcg gacgcagcgg gcgccgcaca gccgttagcg
    48721 cacgataaaa ttcaggaaca tcaccttttc caccgttgct tttcggagca ggcggttgat
    48781 atcctgtttg agtataacgt gcagccgttc gtagtcattg accgaataca gctcgaagtg
    48841 atgacgcacg cgtatggcgg atttgacggt ttcatgaatc tccgggaccc gggaccgtat
    48901 ctcggcggcg ctctccggac ggttccgcac ccccagcgac agttcaatga acaccgattt
    48961 cgacggatcg tctttcgttc gcgcgtatac cggctcgatg gtaatgaacg taagcggagc
    49021 cagttttcca tcgtgcgata tcacgccgct tttctcgcca gccgttgcac aggccagcgc
    49081 actcactgcg atcaggcacg ccactacgac gcctgcatgg cgaatcatat atcctttcat
    49141 ggttttgata tcggatcgtt gcattgagca ccggagactt tttaccgcca atggcatttg
    49201 gtacgggatt ccatgttctc tctgcagcac ctatccctac ctttcctccc cctgcacaag
    49261 gggaagcgcc agtcgggcga atggggcgcg gataattaga accttttcag cagggagtcg
    49321 ggtgagacgg cagtatgtca cagcgaggtc gctcacaggc atccatgccc tcgcgacatg
    49381 cttcacatcc tgtgaagcgc atcaacccga attggctgcc ggagccgcga atacatggac
    49441 gcattgttcg cggcggggtg agcgtgacat actgccgtct cactcggtgc tattgccaat
    49501 gattctactg gaaaaggctc ttatacgcct acaggaatac accctatcca atccgctgcg
    49561 catcccgttt gatgatggta tatcgccgag gatatcggtc cggcgtgcga gcagccttcc
    49621 ggaagagcag accgtcggag catcccagga cgtgctcata gtcatccgat gtcgatggga
    49681 ccaggcccag cgcatgcgca tgagcgagaa tctcctgcgg cagtgagccg acagtctcgc
    49741 gtgaatacac ataacaccgc ccctgcggcc cggcgagcgc cgctcccagc tccaacgtca
    49801 cgtagagcgc gttaaaggcg cgcgacagca cccagtcgta gcgcccgcga tgcgcacgat
    49861 cgtgcgcgat ttcctcgaca cgaccgcata tcgccgtgag gttgggcagc tccagctgct
    49921 ggatcacgtc acggacaaag gccgcctttt tcgcattaga atcgatcagg gtccccgtcc
    49981 agtcgggata ctggatcgcc agcgggatgc cggggacccc cgctccggtg ccgatatcga
    50041 cgaatgttct acgtggaaca ttcaatcggg caaggggcgc gatgctcccg attacgagat
    50101 cgacaaacac atcccccgga gtcttgtgac cggtcaggtt gaagcgcgtc gaggcctcga
    50161 cgacgagtgc ggtaaatgcg gaatatttct ccagtttccg gtcatcgacc atcgcggcga
    50221 ggccggattc ctcgatgaaa cgccgaaact catgttccac gtggaacatc cccgcggttg
    50281 gtccgcgcat tgtccgcgtt ttcctttctt ttttccttct tcgccgcccg ttcgcgcgct
    50341 tccagatgca ccagcaggat cgaggcgtcc gacgggtcca cgccggatat ccgcatggcc
    50401 tgcccgatcg tgcgcggctg cacgcgaacg agcttctcgc gcgcctcgtt cttgagcccg
    50461 gcgatggcgc ggtagtcgac atcatcggga atgatgcgct cctccatccg gttaagccgc
    50521 gcgatcaact cccggtcttt ttggatatac ccggcatact tgatctccat ctcgacgatc
    50581 ggggccagct cagggtccat cgattctccg atgagggaaa gcacgtcgtc gatgccaatc
    50641 tccggccgct tgagcagctt ttctattggg atacggccgc aggggttgat cgccttgccg
    50701 cgcccggcga gggcctgcgc cgccttctcg tcgacaagga ccgtccgtcg cgcgaaatcc
    50761 tcaatgcggg actggatgcg cgcgtatttc tcgcgcatgg cctcgtaccg cgcctcgtcg
    50821 accaggccgt tctcgtagcc atagcgcatg aggcgcgaat cggcattgtc ctgccgcaac
    50881 agaagccggt gctcggcgcg cgaggtgaac aaccgatacg gctcggtgat ctctttggtg
    50941 accagatcgt ccaccagaac cccgatatag gcctccgagc gccgaaggac gatgggctcc
    51001 gatccgcgaa tctgccgcac cgcattgatg gcggccatca ggccctgcgc ggcggcctcc
    51061 tcgtagccgg aggtcccgtt tatctgtccc gcatgataga gccccgcaat gcgcttggtc
    51121 tcgagcgagg gacggatctc ggagggcggg acgaaatcat actccaccgc ataggccggg
    51181 cgcatcaccc ggacctcttc gagccccggg atggtgcgta tcatctcgag ctgaacgtcc
    51241 tcgggaagcg agctggagaa gccattgatg taatactcgt tcgtgttgat cccttcgggc
    51301 tcgaggaaaa gctgatggcg gatcttgtcc gcgaagcgca tcaccttgtc ctcgatcgac
    51361 ggacagtagc gcggcccgat gcccttgatc tttccgccat agagcggcga gcggtgtata
    51421 ttgtttcgga tgaaggcatg cgtccgctcg tcggtgtagg tgatccaaca ggggacctgc
    51481 tcgcgctcga tcgtcgtcgt cgcataggag aatggcgttg ggcgctcgtc cggatattgg
    51541 ggttcgcatc gcgaaaagtc gatcgaatcg ccgttcacgc gcggcggggt tcccgtcttg
    51601 agacgcatga ccggaaagcc gagctcgcgg agcgaatcgg agatcccttc cgaggaaaaa
    51661 tccgcaagcc gtcccgcacg ctcattgtac tcgccgatat gaatgaggcc cttaagaaag
    51721 gtgccggtgc acagaatcac ggcccgcgct cggtgttcct gcccgcgcac cgttacaatc
    51781 cccgtcaccc gtccgctctc agcgattatt cggtccgcga tatcctggat aacggaaagg
    51841 ttctgctgcc gctccagcgc gagcttcatt cgcaattggt acgccttctt gtccgcctgc
    51901 gcgcgcgggg cccagacggc cggtcccttg ctgcggttga gcatcttata gtgaataccc
    51961 gtctcgtcga tggcgcgcgc catctcaccg cccagcgcgt caatctcgcg gacgaggtgc
    52021 cccttcgcta gtccgccgat cgccgggttg cagctcatct ggcatatggt gtcgaggttt
    52081 ccggtgagca tgagcgtatt catgcccatg cgcgcagcgg caagcgccgc ctccgcgccc
    52141 gcgtgccccg cacccacgac gatgatgtca tatgttcgaa tcatgcgact cacgctccga
    52201 ctgtacgttc aggtaacacc gagcacacac acctgatcgc tcttttttca tcctattcac
    52261 tgacggcaag aaatatttat tgcaaggtcc aatgattggc gttgtgcttg cagttggcaa
    52321 tgaccccctc ccgaccttcc ccttcgttcg ggggaggtgt gtcaatagtc attcctgccc
    52381 cctttccctt tacgaagggg aaaaggggac gggggatagg ggtttaatga accattgcta
    52441 attgtgattg cagctttagc gcagtgcgat tttcataacc gggaaatggt atgaacgata
    52501 ggagtaaata catttcgcgc cttcacgtca agaagggcga catcacgaaa gaaagcgtcg
    52561 acgcgatcgt caacgccgcg aacccgagcc tgcttggcgg cggcggcgtc gacggagcga
    52621 tccaccgggc cgcggggcca gagcttctgg aagcgtgccg cacgctcggc ggctgccgcc
    52681 acggcgaggc gcgcatcaca aagggataca acctgaaggc tccctttgtg atccacacgc
    52741 cgggaccgat ctatcacggc ggctccagcg gtgaggcgca ggtattgcga aattcgtatg
    52801 aaaactcaat gcgcctcgca catgaacacg ccctatcgtc gatcgccttt cccgcaatct
    52861 ccaccggcgt gtacggttat ccccgtgagg aggcttgtcg cattgccgtg gacgccgtgc
    52921 tcgattttat ggcaacgcac gactatcaca ttgacattta ttttatacaa tatgataatg
    52981 aaaattatac tatatataaa aactatattg atggattagg aaaatgaact atctcgaact
    53041 gctcaaggaa accgcgaaac gattcaacag catcgtctgc ctcgggatgg atcccgtcat
    53101 cgaggacatt cccatcacca ccgggagcgt gaaggagcgg attgtcgcct tctacgcaga
    53161 aatgctcgac gccatcgaga agaggaaggt ctatcccgcc gcggtgaagc ccaactacgc
    53221 cttctatgcg caatacggcc tcgagggcat cgaggcgctg gtttccgtgt gcgagcgcag
    53281 ccgcgccgtt ggcctcccgg tgattctgga cgtcaagcgc ggggacatcg gcaagacggc
    53341 cgaggcctat gcacgcgaat cgttcgaatt cttcaaggcc gacgcggtga ccctttcgcc
    53401 cttcctcggc tacgattcaa ttgagccgtt catgaagcgc tttccgggca aggggtacta
    53461 tgtcctcaac aagacctcga acaagagctc ggccgagctg caggacgtca gggttgggga
    53521 ggacaccgtg tacgcactcg tatcgaagaa gatcatcgac tggtaccagc cgggtatcgg
    53581 cgcggtggtc ggcgcgacct acccgtccca gctctcggag atccttggca tatacgcgca
    53641 tgccggcaag gaggtgccgc ttctgatccc gggcgtcggc tcacagggag gcgatgtcgc
    53701 cgaggtgatc gccgcgctca agaagagccc gtctgtgcgc atccaccgca tcaattcatc
    53761 cagcgccatc aacttcgcct acaagaagca tcccggcatg cacttcgccg aggcggcggt
    53821 aaacgagctc gaaaggctga atgcggagat caacggattg ctgtaacgta ccgtgaacgg
    53881 ctatactctc agcagcgcca tcatccgctc gtggatgcgg ccgttggtgg cgaggacctc
    53941 ggggtattcg ggatcgaagt ttgatccgtc atagcgggtg acgcgtccgc ccgcctcctc
    54001 ggcgatgatg gcgcccgcgg cgcagtccca gggcttgagc tgccgctccc agtagccgtc
    54061 gatgcgcccg caggccacgc tgcacaggtc cagcgccgcc gagccgaagc gccggacccc
    54121 ctgcacctcc ggcatcaccg aacagaactc gcgggaattg ttgttctccg tctctttctt
    54181 gttgtacggg aatcccgtcg cgacgatcga gatatcgacg gcgtcggtcg tcgataccct
    54241 gatcggcgcg ccgttcagaa atgagcccga cccgtgcagg gcgtgaaaga gctcgtcgtg
    54301 aaagggatca tacacgaaac cggccgccat gcggccctcg cgacgggaat aaacgccgat
    54361 ggacacgcag aagcaggcga taccgtgcgc gaaattattg gtgccgtcga gcggatcgac
    54421 gtaccagacg tagtcgtcgc cctcctccgc ccggctcccc tcctcggcga tgatcacatg
    54481 gccgggaaac tcgcgccgga gcgcatcgaa gataagacgc tccgattcgc ggtcgatgtt
    54541 cgtgaccagg tcggtgcggc tcttgtactc caccaccagg tccggcgccc ggaagccgcg
    54601 caggaggacc gcgccggcat cgcggatgat cccgcgggcg aaggcgtcga tgtgcgacag
    54661 catggtcaat ccttccgctg gtcggcggtg tagaagcgcc agccgatccc gaggtagaag
    54721 gtcatgtcgt atgaaaagcc gatggccaca tgctcggcgg tgcccgtgta ttcgcggaag
    54781 gtgttgttgg tgagattgca cccgaagcgg ttttcgaaca tgagcgcaaa gctcgtgctg
    54841 atggggaggt agtagcccat gattatatac ccccacaggt cgctcgcgag gcgcgccttg
    54901 acggggttgg ccgacggatt gttcaccgcg atgagcgact tgccggagaa gccgatcccc
    54961 atgaagaagc cgcggtcgag cacgtgcttg atggagatgc cgggattgag gaagtcgtac
    55021 tggtaatagc cgatctcgtc ggagatgacg gtgcgctgcc attcgaaatc gatgcccacc
    55081 cacacccact tgaggaagat gttcgtttcc agcatggtcg caaagccgcg catggcgaag
    55141 ctcttgcgct cgagttcgtc gctcatcacc tcgtagatgg ggacataccc ggccttgaga
    55201 aagatgtcgc gcgacaggaa cccggggcgc gtctgttcct cctgcgcctc cttctcctgg
    55261 acgaagcggc ccacggtgcc ctcttcgtac atcgtgcgga tctgcttgcg cgcctcggtg
    55321 agccagccga tcacgttccg gttgtcgggc tcgattttga gggcggcctc gccggcgcga
    55381 actgacgcca ggtagtcgcc cgtcttgtag agcagaaacc cgagtcgctc ccacgaggcg
    55441 acctggcggg gatttcgctc cgtcgcgccg cgatagctgc ggatcgcgtt tttcacgtcg
    55501 cccttcatct cgtagaccca tccgagattg tgcatcacga tggccgatgc ggcgttctgg
    55561 ccctgcaatg attgctcgaa gagctcgatc gcctgatcgt actttttctg gtttcccagc
    55621 tcgatgccct tttcgttgag cgtaggcgtg cgttccgcgt gcagcggcgc gcccagcgcg
    55681 gacatcgcga gcgttaggcc gataagtgtg ccccacatcc gtcccatggc ggaaaggtga
    55741 tgggggatta tttttttcgt caacggataa aatatttgca ttaatgagac ataatcatgc
    55801 actttcatgt cgatagtgta agtaccacgc atcggtatca ttctcgacac ggacggggga
    55861 cgcttccatg aaaaaaacga tcatgatcat cggcgggggc ctcctgcagg acccggtcat
    55921 ccagaccgcc aagaagatgg gcctgcaggt catcgtgacc gactacaacc cgaacgccat
    55981 cggcatgcgg cacgccgaca ttccaatcgt gatgagcacc cgcgacatcg agggctcggt
    56041 gcgcgtggca aaggcgcaga acaacatcac ccccatacac ggcgtactga ccgtgggcac
    56101 cgacgcctcc atgaccgtcg ctgcggtggc gaacgcgctc aatctgcccg gcatcaagtt
    56161 cgaggacgcc gaggcggcga ccaacaagat caagatgcgc aagcgcttcc acgagcacgg
    56221 ggtgctctgc ccgcgcttct acccggtgtg gtcgatcgcc gacgcgaaga aggcctgcaa
    56281 taagctcgga tttccggtcg tcatcaagcc gtcggacaac atgggcgcgc gcggcgtgac
    56341 gcggatcggc accaagaacg aaatcgccga cgccttccag ttcgccaaga gcgcctcgcc
    56401 cagcggcgaa ctcctcatcg aggaatacat ggagggcccg gagctttcga tcgacgcggt
    56461 cgtcttcaac ggcgagatca ccatcacggg cgtcgccgac cggatcatcg agtacccgcc
    56521 gtacttcgtc gagaccggac acaccatgcc gtcgcacctg ccggcggaga tccaggagaa
    56581 tgcctgcgag gccatgcgca agggcatccg ggcgctgggg atcacgcacg gctttgcgaa
    56641 gggcgatatc aagatcacca aatgcggtcc caagatcggc gagctcgcgg cgcgcctctc
    56701 gggcggcttc atgtcggcat acacctatcc gctggccacg ggcgtggacc tcatgcgcgc
    56761 ggccgtggag atcgccctcg ggcaggagcc cgggaacctc gatccggtga tgaaccgcgt
    56821 gtcaatcgag cgcgccatca tcaccacgcc ggggatcgtg aagagcatca cgggcctcga
    56881 ggaggcgctg caaattccgg ggatcgaaga aatcttcatg aacgtgcagc cgggcgacaa
    56941 gatcgtcatc ccgcgctcga acgtggagaa ggcggggcac atcatcgccg tggggaacac
    57001 gctcgccgag gccgaggcgg ccgtcgcgcg ctgccgggag gtgctttcca tcgagctctt
    57061 cgaggcgacc gagctctcgc aggagacgat caacatcgcc gcgcgcgaaa agcttaaaaa
    57121 aacctgctat gtgtgcaaga attgcgacgg caaggactgc ccctccggtg ttcccggcat
    57181 gggcggcgcg gggacgggcg cctcgttccg ccgcaatgtc gagtcgctgc gcaactacaa
    57241 gatcaacacg cgccttatcc acggcgtgac cgcgccggac acctcgacga gctttttcgg
    57301 gatcaatctc tcgtttcccg tcatggcggc gcctatcacc ggcaccatga ccaacatggg
    57361 cggcgcgatg gacgagctcg actataaccg cgccgtgatc gacggctgcc tgcaggcggg
    57421 caccatcgcc ttcgtgggcg acggggccac gccgaacaag tacaagatcg ggctgcaggc
    57481 gatcggcgag gccgacggga tgggcgtgcc catcttcaag ccgcgcagcg acaaccgcga
    57541 gctcatcaag cggctccgtg cggccgaaaa agtccactcg gtggcgatgg gcatggacat
    57601 cgacgcggtg gtcttcaaga ccatggcgct gaagaaccaa cccgtgggcc ccaagagctt
    57661 cgacgacctc aaggcgctca tcagcatgac cgaatacccg ttcattctca agggcatcat
    57721 gaacgtccgc gacgcggtga tggcggtcga ggtgggcgcg cacggtatcg tgatctccaa
    57781 ccacggcggg cgcgtgctcg acgagatggt gggctccatg gacgtgctcg aggagatcgc
    57841 ggcggcggtg aagggacaca tcaagatcat catcgacggc ggatttcgca ccggcgtgga
    57901 catcctcaag gcgctcgcgc tcggggccga cgcggtcctc atcggccgtc cgatcgccat
    57961 cgcggccgtc ggcatgggcg cggccgcggt gtcgtactac ctgaacggcc tgcgccgcga
    58021 tctcgagcag gcgatgatcc tgaccggctg caacggcatc agcgacatca ccagcgacgt
    58081 ggtccgcaag atcaacggga aaacgctgac atacgtgtag gatcgcgcgg tcggcgtgtc
    58141 gtgatcgctt cggtgattcc agtgaggacg gcctcgtgcc gaagggaacc caatccccat
    58201 gatagtgact gattcacctt ttatgaaccg cggtcttcgc tgtgagggat ggcttttcct
    58261 ccccgatacc gcaaagaaac cgccggtagt cgtcatggcg cacggttttg gaggtctgcg
    58321 taattaccga ctcccggcct tcgcggagcg ttttgtgcag caggggcttg cggtttacct
    58381 gttcgagtac cgaaattttg gcgacagcga aggggagccg cgcaactgga tcagcccacg
    58441 gcgccatctc caggactggc gtgccgcgat tgcgcatgtg cgtactctcc caaatgttga
    58501 cgggggacgt atggcccttt gggggacctc tttcagcggc ggacacgtca tcgtgaccgc
    58561 ggcccgggtt cccggaatcc gggcgatcgt gtcacaggtt ccattcgtcg acggcattgc
    58621 gagcatctcg ctgatcccgc tgcgctcggt catcgccggc ttcatccacg gtctgcggga
    58681 tcttgttcgt atgatcacat tccggaggcc gcacctgatt ccgatcgtcg gcgacgcgac
    58741 gctcggcgtt ttcggggccc tcaacgcgcc cgattgccgg gaaggggtcc tggccatcgt
    58801 tccagagggc gctccgttta ccaatgaatg tccggcccgg atctgcctca ccctggtaca
    58861 gtaccggccg atacggtacg ctaaaaaaat tccctgcccg gccctgttca tcattgccga
    58921 acgcgattcg ctcatccctg cgaaagccgt ccgcacaaca gtcgcgcggg tttcgaacgc
    58981 gcaatccgtc gagttggatg ccggtcactt cgaggtctac gtgggcgatc tcttcgagaa
    59041 ggtcgtcgcc atcgaggcga attttctcgc gaaacaccta ctcgcatagc atcgagacgc
    59101 gcttctcgga ttcgcgtaca cccattgcat tgatgaacgc aaccagcgaa tgcccccacg
    59161 gtctctccag tggaagcgac gccaatcatc ccatgacgtc aaattctgtg atatcctatg
    59221 tattctcgtg tgcgttgaat actatttgta taccaccgca agcttctatc tattgatgta
    59281 aaagttccaa ttttttctac tccgtcatga aggcacacaa ctcgtccaac ccggcacgct
    59341 ccgcatagtc gaccacccag cgcgcatatc gtccgaatcg tcgcacccgc tgcatcgcgt
    59401 ccgcgcacgt cacctctgta agaattccgg catggagcag cagctttggc accagcagcg
    59461 cgttgatgaa gggaatgccg ctcgcccggc agtaatctgc cgctttgccg tcgtccgtga
    59521 gaatgaaatc gccccggccg gcgcggtaga gccgcacgca ttcggcctcg ccgccgcgaa
    59581 gcgggatctg cacatccgca aacgacaggc cgtcctcgac ggtgatgctg ccctctgcgc
    59641 agtatgcccg gaatcgctcc gcgccggttt tccctttgat gaccagttcc tcgaaaaccg
    59701 atgccgggac gacgacgcga tagcgcacga cgaatgcgcg cactagcccg gccttgtaca
    59761 ggatgatgag cgacgaggcg tcacagaggg ccgttttcat gatggcgcat tgcggttgac
    59821 aaaaacgttt ccccggcatt gggttcatca aacaggtacc acgcgtatgc gcatctggca
    59881 atcccgattc ttccgatatg cgacgaaaac cgtttcgtgc acatcggaac gttctgtgga
    59941 tgttgatgta cacaattgcg catcattcaa ttgattatta agagggatga tgaaaaccaa
    60001 agcgccatta aaaccgaaga agcgaaaccg gtcatcggcc gcatcgaaac ggttgccgca
    60061 ttgggttcac ctcatcgcgc aggacggata cgataccttc gcgatcgccg cgaagatcaa
    60121 agcggtgatg gaccgcgaat cggtttcggt cgccggcaag tccgtgttcc tgaaggtgtc
    60181 gttcgtcttt ccggtgcgcg atcccgagcg ggtgaagcac atcatcacga accccgcctt
    60241 cattgcgggc gtcgcgcagg cgctggtgga gcggggcgcc tcggtcgtgt atatcggcga
    60301 cgccgagacc ttcgccacgg cgcgctattc gttcgatatc gtcgatatgc agaaagcgct
    60361 aaaagcccta cccgaatcta ttcggaagaa cgtcgtgtgt tgctatctcg atgaggttta
    60421 caaggaatgg atcacgcctg acgatccctt ctgccctgag atcaggctcg actacccgaa
    60481 gatcgtccgt gacgtggacg tgtacatctc gctgccgaag ctcaaggtga acatcttcgc
    60541 cgacatcacc ctctcggtga agaacggcat gggcctcatc accaaggaga cgcgcctgcg
    60601 gtatcacaac gagaacctcc acgcgatgat cgccgacatc ttccaggtgc ggccgcccga
    60661 ctacgtgatc accgatgccg tgttcgccgg cgaggcgcaa ggccccatgg aggccacgcc
    60721 ctatccgatg aacctgattc tcttcgggaa caatggtccg tcggtggaca cggtctgctg
    60781 caacctcatg gggtacgatc cgcgcgaggt gaagcacctc atgctgctgc atgagcgcgg
    60841 ctttggcccc atcgatctta aaacggttcg tgttgagaac gccaagctcc tcaatgagcg
    60901 acggcacgtc ttcacgcggc caagcatgga gcttgcgcat ctctcatcga acatcacctg
    60961 ccatcaaggc gagtgctgcg cgtccggctg cctgcccttc gtgcgcgccc tccttgacgg
    61021 gtacgggctc aaccgcggct gggaatcgct gggagaatac accatcatca tgggaaagaa
    61081 cctcgatatc ccggaagcga cgctaaaaaa gctgaaaaag cggaaaacgc tcgtgtacgg
    61141 cgattgcgtt gagcgctatc ggaagtacgg cgtgttctat cgcggctgtc cgccggatta
    61201 catcaatgcg ctcattacct tcccgattcg cacgtcgctg ggaagaacac cctggctcgg
    61261 ctatgtggaa tactggacgc gcatcaagac cgatctgctg cacctgctgt cgaagctgcg
    61321 ggggaagaag taccggacgc ttcgtggacg agtcaaaaag tgagccgttg attctaagta
    61381 tttcgagcat ggcaaattgt acgtgacact cgtggatctt tacggtaggg tcggatcaga
    61441 tccatcaacg ggaggcccta tgtttttcat taagtatttc acgggacagc cgagcgagta
    61501 catcatcaag ttcggaaggg gacgcatacg caaggaaggg cagggtatca gtttttttta
    61561 ctgggcgaaa agaaacacca tcgtgtcaat tcccgccacg acacgggatg tggatttcat
    61621 cttcaacgag gtgacgaaaa ccttccaggc cgtgtccata cagggacaga tcacctatcg
    61681 catccaggac aagggtaaaa tattcgcgaa tctcgatttt tccatcaatc ccgtcacccg
    61741 cgcctatcgg tccgaagacc cggataagct cgcccagcgg atcatcaacg cagtgcagat
    61801 gctcacgcgt gaacagatcc aggggctggc cctcgaggac gctctcggaa gctcccaggc
    61861 catggcgcac ggtgtaatgg aaaagcttaa aaccgatgcg cgtatcaacg ccatgggggt
    61921 ctccgtcgag agcatctatt tcacggcggt gactccgatc ccggaaatcg ccaaggccct
    61981 ggaggcggaa taccgcgaga tgctccagaa gaaggccgac gaggcgatct actcccgacg
    62041 cgcctcagcg gtggaacagg aacgcatcat caagcagaac gagctcaact cggagatcga
    62101 gctggagaaa cggcgccagg aactcgtgga gcagagcggg gccaacagga taaaggaggc
    62161 cgagtacaaa tcaaaggcca tggaactcga gtggtcgccg tatcgcaatc tcgatccgaa
    62221 gctcctcgtc gccctgggaa tgaagtccct cggtgagagc ggcgcgaaga tcggcaacct
    62281 caacctgaac ccggacctta tcaccctctt actgggcctc gacaagaaat gacgagcgcc
    62341 ttcgacaaga tcgtcatcgt cacgaaggag acgcagatgg accagctggt ccgcgccttc
    62401 gcgacgaagt cgcaggcgaa attctacctg gcgcaggcgg cgcaggccgc ggctcccgtc
    62461 aacgcgcaat cgacgcagaa aaaagcagga agcgcaaaaa acatccaggc gcatcaagtt
    62521 aaatcggagt tcgacgagat cagcctggcg gacagtgcgt acaaggaagc attgaagaac
    62581 cttcagtcgg ccatccccgc gggaatcaat gttcagcata tcgattggag ttacctcccc
    62641 acttttcttt tcggcgaaaa ggacctggtg atcgccctga ggcaggacgg tctggtaatc
    62701 aatatcgcca agtacctgag cgaacagccg atcgtagccg tcaatccaga tcccgcacgc
    62761 tacgacggcg tgctccttcc cttcacggtg ggggatttca atcatcattt cagacgcatc
    62821 gtgagcgggg agttcggcgt gtctgccatc accatcgcaa aagcctcgct caacgacggg
    62881 cagacaatcc acggcgtgaa cgacatcttc atcggcccgc ggacccacac ctcattcagg
    62941 tgcaatgtga gcttcatggg cagggaggaa taccaatcgt cgagcggtat catcgtctct
    63001 acggggtgtg gctccaccgg atggttccgt tcgatagtcg agggtgcgcg gaggatatcc
    63061 tccggattcg acaacgcgaa gcccgcgaag gagcagagca gatttccctg ggatgcgaac
    63121 catctctttt tcagcgtgcg agagcccttc gagagcaaaa cctcgaagac ggacatcaca
    63181 ttcggtaaaa tcacgacggg ggatcccctg gtggtaacgt ccctgatggg gcgcaacggc
    63241 atcatcttta gcgacgggac ggaaagcgac ttcatcgagt ttaactcggg gaaaatcgcc
    63301 acaatcacac tgtcggaccg caaggcgaag ctaatcaaga aatgagattt gtgtgataac
    63361 agttgcatcc cgttcgcaca gtaccctgat caattcctgg aaacaacatc tggaaaatta
    63421 ttactgcggt tggaccaatt ttttattgaa tgcatcgcaa cggctcggta agatctaccc
    63481 gagtgaagca tgatggaaac cagttcacac agatcaccgg cgcgttcgtc cgcgctgacg
    63541 atcgccatcg cgacggtcgc gatcctcctt cccctgtccg gcgccgccct cttctccagc
    63601 gtcgatcggg gacggttgtg cgaagaccgc tgcacgcaga aattttggac gtgccgcgag
    63661 cgcgcgttca actacgaggg caagcgcggt gatcgcgcga tcgccatctg cgacacgatg
    63721 ttcacgaatt gcatgggacg gtgccggctg tattcgcaac cgaagcgccg gtgataatcg
    63781 tggtgtgtgc ggttacgaca gtccgcgaat ctcgcacagg tccgcaaagc tcaatacgaa
    63841 ctcacggaag cgatggagct cctcggcggg gtagggttga taattcgcca catcgtcatc
    63901 gagcgttttg ctgttcacga actgctgtaa ataatactgt ttcacgcgcc cgatctcgcg
    63961 cttgatcgct acgaaatcgt ccagcgtgac gaaaaacggg atgcaggtgg tccggatctc
    64021 ataatcgatg ccggcgtctc gcacgatcga gatggttttc acgatgtctg cgaatcgaaa
    64081 cgttgttccg gtgagaaaat catatttcga cggagaggtc ttcacgtcga tggcgacgta
    64141 gtcgagcagg cctttttcaa tgagccgctt gatgacctcc gggttcgatc cattggtatc
    64201 gagctttacg gaaagcgaga tatccttcac ggacttgatg aagctgtcga ggtttttcgc
    64261 cagggtgggc tcaccgccgc tgattgacac cccgtcgatg aaatgcctgc ggcgctcgac
    64321 gaacgagaag gtctcctcgt tcgtcagcgt ctcgagcgcg cagttattgc acgccaggtc
    64381 cggattgtaa cagtaccggc accgcagatt gcagccgccg gtgaacagta tcgagcaaat
    64441 cttgccggga taatcaatga gcgacgtttt gtgtatgccc ttgaaattca ttgctcgtcg
    64501 cccgcatgat gctgtgtaat gcgtattcct tcctgtcgtt gaactcttcc cgtttcccct
    64561 tgttccactg gttgaccggg cgaaagtatc ccaccacgcg ggagtagacc tctacgggaa
    64621 cctgcttttt ctgtttcata tgcctctcct ttcccatttt atatattatc gattcaatga
    64681 agcgctacat cgccactttt ttatcgcccg ccgcgggcgc ctcgatgccg aaccgctcca
    64741 gctcctcgtc cgtgtgatcg taggggcaaa acttgtgcga tccgggtatg tacccgtgca
    64801 cggggcacac gctgaaggtc ggggtgatgg tgaaatacgg tatcgagtag ttttcggtga
    64861 ttttgcggat caacagcttt acgatgttcg gatcgtcgat tttctcgccg atgaagcagt
    64921 gcaccaccgt cccgcccgta aacctggcct ggagctcgtt ttggtggtcc aggacctcga
    64981 agacgtcgtc cgaatacccg accggcagat ggatcgaatt ggtataatac ggctctccgg
    65041 cccccgcggt gacgatatcg gggaacgtgt cgcggtcgtg tttcgcgaac cggtaactca
    65101 cgccctcggc cggtgtcgct tcgaggttgt acaggttccc ggattcctcc tgataggacg
    65161 cgatgcggtc gcgcatcata tcgagaacgc gcaccgtgaa ggccttgccc tcctccgtcg
    65221 agatgtcctt cccgatgagg ttgatgcagg cctcgttcat tcccaccagg ccgatggtcg
    65281 agaaatggtt cacccagaac tttccggtgc ggttgtacac atcggccaga taataccggg
    65341 tgtacgggta gagattgctc tgcgtgaagt tttccagcac cttgcgcttt atctccaggg
    65401 acgacttcgc catgtccatg aggcgcccca gcgactcgag gaactcgtcc tcgctcgccg
    65461 ccttgaatcc aaggcgcggc atgttgatcg tgaccacgcc gatgctgccg gtgagcgggt
    65521 tcgcgccaaa gagcccgccg ccgcggcttc gaagctcgcg attatccagg cgcaggcggc
    65581 agcacatcga gcgcgcgtcc tccgggtcca tgtcggagtt tacgaaattc gcgaaatagg
    65641 gtatgccgta cttcgccgtg atctcccaca gggtcgtgag cgccggattg ttccagtcga
    65701 agtccgcgcc gatattgtag gtcggtatgg gaaaggtgaa gatgcggtgc cgcgcatcac
    65761 cctcgagcag gctctcggcg aaggcgcggt tgaagatgtt catctcttcc tggaattcct
    65821 tgtactgccg gtcgcgcacc tcgccgccga tgacgacgta ctcctcgccg atgttcttgg
    65881 gcactttgag gtcgagcgta acattggtga acggcgtctg gaagcccacc cgcgtgggga
    65941 tgttcatgtt gaagatgaac tcctgcaggc attgcttcac ctcggcatag gtgagcccgt
    66001 cgtagcggat gaagggcgcg agataggtat cgaagttcgc gaaggcctgc gcgccggccg
    66061 attccccttg cagggtgtag aagaaattga ccacctggcc cagcgcgctc cggaagtgct
    66121 tcgccgggcg gctctcgacc ttcccgggca cgccgccaaa tccgcgccgg agaaggtcct
    66181 tgaggtccca cccgacgcag taggccgaga ggagccccag gtcgtgcaga tgcacgtcgc
    66241 agttgatgtg cgcgtcccgt atctccgggg ggtagatgcg ctcgagccag tacttcgcgg
    66301 tgaccgccga ggcgatatga ttattgagcc cctggagcga atagctcatg ttgctgtttt
    66361 cgttcacgcg ccagtcggat cggtcgagat acttttctat gagtgcgatc ccctcgttta
    66421 ggaggtcctt gccgtcgcgg atcttccggt gctgttcacg gtagagaatg taggccttcg
    66481 cgacctttgc gtggcccttc tcgatgagca tcttctcgac gaggtcctgg atgttttcga
    66541 cggtgggaat gcgctcgttg caataagcga tctcgacgat gtcgcgcacg gactgcgcca
    66601 cgccctgggc gatgtcgcgg ttgtcgccgc cgacggcccg cgccgcattg aagatcgcat
    66661 cgacgatttt ctcgaggtcg aaaggaacaa tccgcccatc gcgcttccgg atgaattgta
    66721 cctttttctt ctgtgcgccc atgaaggata ctcccgaccg ataaactctt ggaccgaacc
    66781 gaaacgcggc gaaagaaacc cgccggagca gcgtacgcat cgtcgcgaga tcgcgaatat
    66841 ccggggaacg atgaccgacg gccacccgag acgaatattc gaaaacacga caaccactcc
    66901 gctctgtgga caagcgaaga cgcgacatga tgcgtggagt gcgttctgtg tgttaaaaat
    66961 actaccggta cgcgcgcttg ccgtcaatta aaatattatg tctcttaaaa aaaattcacg
    67021 gccagcggcg catactccga tatatatcgt gtattcattc aagcataatt tcttccgccc
    67081 gtgccggcgg cgtcgttccg cattttcatc tatcgggggc taatccatga aatacgctat
    67141 cttcgggact ttggcgctga tgtcgatttt tttgctcttc gcaaattcat cggccgaggt
    67201 gggcgccgat atcatcgaca aaatcaagct gacggacgcc gatatccccg cggactttgc
    67261 ctacggtgtg cttcccgatc gcgtaaagcg ggttctgaaa ggcaacccgg cccagttgga
    67321 ccgcgcggcc atcagaaaag taatcaaaga catggaattg tatccggggg gcgatcacac
    67381 cacggtgctg gccatgcacg ccgccgtcat cgcgcacaag aaaaacccgc acgatgatga
    67441 catcgtctgc tatatcatcg tatacaagga tatgaaatca gccaagcacg agatcgaaaa
    67501 actctctgcc ctcgtgggcg ccaacaagac gcgaacggtc atggtcaccc gcgacaactg
    67561 cgcggtgttt ctgttcgtcg atgaggaggc aaacttccat catattcaaa aaatggcggg
    67621 taccatcacc gcgcgactca aaaagtaatc aatccctcag gagcgagcgg tacagggcga
    67681 tctttctttt tacgtactcc gatgaatact cgttttcgaa tgcagaagaa tcgtttctga
    67741 tggactcata gacctgcgcg atcgttttcc aggcccgctg caactcgatc cgtcggttgt
    67801 gcaccgtccc aatccccaca acgaacagct ttttgaacag aagcgacgtg aagtacctgc
    67861 catgcggatc aaatgaaaga tcctcgccct cgagccccgt gctcgtcgtc atgccggtcg
    67921 cgagatcgtg cagcatgatt acctggtcct tgaggatggc gaggttcttc ccgttgttcg
    67981 aatacgacag gttcgtatta aacgactgcc tgaccagcgg atagcttttc ccggtattga
    68041 tcgcatacag gaccgccgag aagttcccgg tccagccctt tcggtacgcc acccggttgt
    68101 tgtcgagcca gtgaatctcc gaaccggagg tgatgccctt tatgac
//
LOCUS       DAORHF010000002        56844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_231_length_56844_cov_6.482893, whole genome shotgun sequence.
ACCESSION   DAORHF010000002 DAORHF010000000
VERSION     DAORHF010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 56844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56844
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_231_length_56844_cov_6.482893"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PKJ16_00290"
     CDS             <1..301
                     /locus_tag="PKJ16_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45447.1"
                     /translation="LVKEQKLSVSGFIDPREAVAIGKLSGADTIVTGTGHLVSRDSDV
                     LSYLVIKVISLRSGSVIMTANLRKNMSISDAVTEMSRSIKKCLDERAAAQKGAPK"
     gene            424..1254
                     /locus_tag="PKJ16_00295"
     CDS             424..1254
                     /locus_tag="PKJ16_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNV45448.1"
                     /translation="MRTIRDILAAEATVVSFVVLNAVAIFMTPLFAHGSTWERVISHV
                     DFVCVLYFMLESAIKINRDGWRGYIDVGWNRFDFLIVLCSLPVLLEPFVDLKGFGIVM
                     VLRLGRLFRLFRVMQFIPNLDRMAAGIGRALRASVGVLMAIVIVNIIFALGGTLLFGE
                     FAPKHFGNPLMSMYTTFKVFTVEGWYEIPDMIAANTDSDLMALGVRVYYVVAVVVGGL
                     IGLSLANAVFIDEMTADNNKLLEEKIDELSRELRELKGMLADREHPRGSGRARRGPRT
                     "
     gene            1251..2879
                     /locus_tag="PKJ16_00300"
     CDS             1251..2879
                     /locus_tag="PKJ16_00300"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HNV45449.1"
                     /translation="MTAETALHLVGGVGLFLLGMTLMTDGLKTLAGDSLRRALARFTG
                     GRVTSVISGAGVTAVIQSSSATIFATIGFVSAGLISFEQSIGIILGANLGTTSTSWIV
                     TYLGFKFKIGIVSYPLIALGALLKLFASHRFSAAGIIMAGFGMIFAGIEMMQAAMASA
                     AVFNLAGIGGATLPERIALLGLGVAMTLLLQSSSAAIAVTLAALGAGSITILQAAVLA
                     IGQNIGTTVISSIGAIGAATQAKRTALAHVAFNLITGASVFLLLPWFVAASGCIARLI
                     GRGEDIIALSAFHTLFNVAGVLAILPFTSPFARLIERMLPERGPALTRHLDASVLMVP
                     QVALETVRRTLADIAAYVIGHLLSLFQGYGQMAAMPETLDTARSALDRTRFFLQDLRS
                     FNNSPLLHERHANIIHALDHLYRLIGACRETNHLRGALANPELREIALHLADRMELLL
                     GWLEGGSGSDPLGDLEQASFSIAGIRKEQRKEILRRTSLGEIDTEAAFGIIESMRWLD
                     RIAFHIWRSVVHLENITRAHDRSEIAADPDGYGR"
     gene            complement(2867..3871)
                     /locus_tag="PKJ16_00305"
     CDS             complement(2867..3871)
                     /locus_tag="PKJ16_00305"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV45450.1"
                     /translation="MSRSGDSGMAFSALESALLNRIQSDFPLAERPCREIAAAIGADE
                     NEVRDTLSSLRARGIIRIIAGIFNAESLGYETSLVAFEVDGADADDAARIINGHPGVS
                     HNYLRDHHFNIWFTLAVDRATSLHKTVESLAERVRATRHLILKTEMLYKIGLNLRIGD
                     GAAGATLAHAINPAPPRALSADEREAVRLLQIDLPDADEPFRALIDLNAGTIHQGALV
                     GIARALRDEGVMRRYAATVRHREAGYTANAMTVWRPECAMNDVIDVFIDEPAISHLYK
                     RTVYPHTWEYPLFAMIHARTERDLADTVSRLSRASGITDYLVLKSLREFAKRRVVYRP
                     "
     gene            complement(3837..4907)
                     /locus_tag="PKJ16_00310"
     CDS             complement(3837..4907)
                     /locus_tag="PKJ16_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019241290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV45451.1"
                     /translation="MKASSGPRLIFWELTKRCNLRCRHCRAEADDAAFSGELPTGAIT
                     GIIDDIAAFAKPIMVLTGGEPLFRDDIFAIARHARGRGLRTALATNGTLIDEAAARAI
                     RDAGIMRVSISIDGSTADSHDGFRGIPGSFELALRGAERLAAAGVEFQFNTTVTRRNT
                     AELEEILRLAEDRGAAALHLFMLVPVGCGVEIADTDMISPDEYERILNWFYDRSRETA
                     LEFKATCAPHYYRIVRQRAKAEGRSVAFETNGMAAMTRGCLAGTGVCFISHRGDVQPC
                     GYLPLVAGNVTQTRFADIWNDSPLLVGLRDLSNLTGKCGACEYRGVCAGCRARAFHAT
                     GDHFAEEPYCVYVPQRRQRHGL"
     gene            complement(4904..6205)
                     /gene="hemA"
                     /locus_tag="PKJ16_00315"
     CDS             complement(4904..6205)
                     /gene="hemA"
                     /locus_tag="PKJ16_00315"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HNV45452.1"
                     /translation="MSAETKIPEIAVIGLNHKTAPVDVRERLSLPDEAVRDLLARSRE
                     RGIDEIVFLSTCNRVELYFAASDIPRAIDGLLSLLDDRIDIDRRRLEPCLYRKYSRDA
                     AAHLFSVASSIDSMVIGENEIVGQVKDAYRCAVSAKRTGPLLNRLFHQAFKTAKRVRT
                     ETEIAHNPLSVAFIAAELAKKIFEDLSRRSALLIGAGDMGELILKYFTKSDIREITIA
                     NRSLHNAERIAEEINRRAHIIPLEDITEAAARADIIIASSGSDAHLITEAMGREIARR
                     RRNNPLFVIDIGVPRNVDPAVGALDGIYLYNVDDLRAIADENLKSRMKELEVARQFVE
                     ADADDFMVWYERLDTVPLISAIQERFDEIRRAEFERYRRRRLKHLSDADLAIVEDLTR
                     QIMTKTLHNPIQAIKRARAEDGPRHRDRAAAKKLIQDLFDS"
     gene            complement(6207..7046)
                     /locus_tag="PKJ16_00320"
     CDS             complement(6207..7046)
                     /locus_tag="PKJ16_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45453.1"
                     /translation="MHSVMFYIALALFSVASLVSVAQFFTDRAAVHALARWTLYTASA
                     VLIATGIVRFGPLSAETLLRAVHGIWAYFFLTALVMICVLAYLPFSRWRAQVRSIGAI
                     AVPFITMLLFASVPFFGSDRSMAVITAHGLLPLHIVLTVVGELLFFLSAAGSLCSIVA
                     SRQLKRRGSMRFLNRLPALESIDGFNRWTALRSLAFISAGLVTGLALLWLQYETVSMY
                     THKEIVIYVSWAAILAVALLRARTGVSTNLINRITIALFLSIAAAFGYVNFFITTGFH
                     SFQ"
     gene            7289..7969
                     /locus_tag="PKJ16_00325"
     CDS             7289..7969
                     /locus_tag="PKJ16_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45454.1"
                     /translation="MFDIMDYLPDNDPAPLEGRCVDVAPQYADDEIAVEIDALLAARF
                     DPLYRRDKFKVGAAGVEIIHPALAYPGRRALVKGPDYVRFLLSLYPQKSDLESVEKIV
                     LRPRHVELGDIELMALYLRNRRILVLYLYVPHFYVVGESKFREYAEFAPCKLNDIVGG
                     SQTGPGRMAAASSTVKVPPLWYILSMISPSPDPGVDKFLVKKEREHYEKISAMLDEVS
                     YFYARHGY"
     gene            7973..8767
                     /locus_tag="PKJ16_00330"
     CDS             7973..8767
                     /locus_tag="PKJ16_00330"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HNV45455.1"
                     /translation="MALVDYHVHTPLCGHAHGEPAAYVEHAIAVGLSEIGFSDHAPLP
                     AGMREGITMLPEETERYIAMIERVRDEHRGRIPVRLGFEVDYPLHDTFDRRYLDDPRI
                     DYLIGSCHFVDGWAFDHPDGVAEYNRRDVDEVYAAYYDTLIELANEGRFHIVGHFDLV
                     KKFGHRATRDHHDRIRRVLMALARCGTAVEISTAGLRKPVGEIYPAPDIVALLFEMNV
                     PVTLGSDAHAPEEVGYEFARARELIRGAGYRRIARFEKRVRRDVPI"
     gene            8879..9175
                     /locus_tag="PKJ16_00335"
     CDS             8879..9175
                     /locus_tag="PKJ16_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45456.1"
                     /translation="MKYLTLCAVIVLALALAVSCKQAPKDMSLDDFLKIQNEIIKTDL
                     TPEAKDKVVTKFGYTLKQYDDLDEKVKGDPKLQEKLGEIRLNMGKNKDIKKEFE"
     gene            complement(9206..10054)
                     /locus_tag="PKJ16_00340"
     CDS             complement(9206..10054)
                     /locus_tag="PKJ16_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV45457.1"
                     /translation="MAIDFHTHLFTPEIVASRETGMADPAFAALYANKRAKIVGHDGL
                     LAGMDDAGINSAVVMGFPWTDEDLRRRQNDHFAELARIELGVLFFFGSVPMDPATTSE
                     EIDGAVRLLREQGMYGIGEIAYYGSGLTEAAASHLRSVLAAADTHGMPVCLHVNEPLG
                     HRYAGKISNDFGRLYDVIADHPAVTIVLSHWGGGVLFYELMPEVRKAFARVYYDTAAS
                     PLIYDNRIYASAVSLVGSGKIILGSDYPLTGFARYIAPIKKLTDARDRDTILRDNARR
                     VLGLIE"
     gene            complement(10056..12275)
                     /locus_tag="PKJ16_00345"
     CDS             complement(10056..12275)
                     /locus_tag="PKJ16_00345"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HNV45458.1"
                     /translation="MNSVTITIGGMSCVMCSRAIEGALASLPGVASAEVDYATGIAIV
                     HFSGAPVPIDAIAAAVERAGYSFLGTADDAPSFDDEETARELRSRKVRFVAGLAVALP
                     LMALMYLPAAKMIAHSPFVFLAALPVFLFVSAPIFAGAVRALRNRALTMDVMYALGMG
                     VAIVASALAATGVLSMEFMLVETAIMLAAFLMLGKYLEARARGKTSTSIRRLMDLHPK
                     SATVIRDGVESTVPADAVRAGDTVLVRPGDALPVDGTVIAGESAVDQSMLTGEPVPVH
                     RGPGDRVTGGTINGNGLLTVRAERIGRDTVLAGIIRLVRTAQGARPPVQRFADRIIVW
                     FIPTILAIALTASAAWFFLLGGTAIFSLSVLIAILVVACPCALGLATPTALTVGIGRG
                     AELGILVKSGEALEQSERLTAVMIDKTGTITMGAPQVSAVESFDIDRLELIALAAGAE
                     AKSTHPFASAILMHAAASGIMPDPAEGLVAFGGKGISARVAGRTVLVGSEPFLRENGV
                     AIPNAASTAITRHALDGASLVFVAIDGVLRGLFAVRDPLRVNARAAVDALHSMGLEVI
                     LVSGDGGHAVAAVAAEAGIDRHYSSALPENKAARIRALQERGGRVAFAGDGINDAPAI
                     AQADLGIAMGGGTDVAIETGDIVLLGADPLGIPGAIELGRAVMRRVKQNLFWAFAYNL
                     LLVPLAAGVLTPVAGITFRPELAGLAMAASSVTVVGLSLLLKRYTPPSLRRAARTGN"
     gene            12464..13300
                     /locus_tag="PKJ16_00350"
     CDS             12464..13300
                     /locus_tag="PKJ16_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNV45459.1"
                     /translation="MVQMKFAIARSDEEDFSDALFDEGADSVAVENAPADDAVVLTAI
                     FREMKTLREELAWRLTEVAELEESVWKYRWLEHLEGVALTDEVYVQPTTSSGLVPREY
                     PYIILIDPRDAFGDGHHPTTALCADALSTITRSVPIERRAQMRVIDVGTGTGVLAVLA
                     ALLGYGRVDAIDIDPESVARARENFARNECARIGAACAQVAEHAADAPYDIVIANLLS
                     GIITDNIDHLRRLTTPGGTIIASGVSDQWHDEMTGLFRTHRLVVHRHDRKDGWNGYVV
                     GA"
     gene            13326..13841
                     /locus_tag="PKJ16_00355"
     CDS             13326..13841
                     /locus_tag="PKJ16_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45460.1"
                     /translation="MDRIEAQLIIDILKTGSRYYRPLDREFSSEDEGFFIWYRPESDD
                     FVVRRYETDDWAVHRPGCDEEMVVSESVLMRLFTEYGRDNALWPLYDTNRYRFDFDTP
                     QPRKTIGQAIRESMHASDLGSLRCSHCASRDVEPTEYSSDVNPARARRYVHLAVECTR
                     CGRTSVYEWDD"
     gene            complement(13859..14284)
                     /locus_tag="PKJ16_00360"
     CDS             complement(13859..14284)
                     /locus_tag="PKJ16_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HNV45461.1"
                     /translation="MNTQTHTTIPHDPAIYAFRTTGARDGGQLPDDASSLEDDADKRP
                     LRCRICGAIITHRSAAMSMQGAHRHVFSNPAGISYEIGCFSHAPGCVTAGAPTEEYTW
                     FAGYAWSVALCGGCFNHIGWCYTDGGGGFFGLILAMLAE"
     gene            complement(14250..14435)
                     /locus_tag="PKJ16_00365"
     CDS             complement(14250..14435)
                     /locus_tag="PKJ16_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45462.1"
                     /translation="MSDAYDRGPWRLMRQDEHGRACLVDTFDEYEPAVRKMSLYESRG
                     HKQVYWIEHADAHDHSA"
     gene            14599..16425
                     /locus_tag="PKJ16_00370"
     CDS             14599..16425
                     /locus_tag="PKJ16_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV45463.1"
                     /translation="MNAKRFICAILSMSLAAAMLYCGSPIPRASDDTSATDARRDTSA
                     RKTDPARPSDLALDEKEYKSAAKRGDKKSNGRSRSDADAPSGESKPSTAKRERRPGAS
                     GLKAGFVDDNEQFNRYLEFLKKFGNTARNIPFDIGERIIIRVRDANGKPAANALVTVK
                     SGYRTLLTARTYADGTVLFFPVEYGSQSSYRATAEFQQQSADATFERTGKREVVIQVK
                     TPRAVMQRVPLDILFVFDTTGSMGEEINRLKKTLEIIYLNLTALSTKPLVRFGMVLYR
                     DRGDDYVTKVVPFTEDIDAFQKELAEVSASGGGDYPEDLQEALHDAIKKMKWNRDGIR
                     LGYIITDAPAHLDYGQSYTYTHAVKDARANAIKFFSIGTGGLTIDGEYQLRQIAQYTY
                     GRYIFLTYGEKGDSAGGAPGSVSHHVGANFETDKLESIIMRFARKELSYLTDKPLDDG
                     EGYFQANRIGTEDKKDTVKKLFTMAIGQLVDFSSIAIAPKTRAAVMPIGFDAKNAPLK
                     LNAEYFTEHLILSLGSSKKFTMVERKDLQRIMRELELQMAGLVDQGNAAKVGKMMGAE
                     VLIYGQLYRKKDHYELFIKLVRVETAEVLSVTRARIDTSLGL"
     gene            complement(16411..17208)
                     /locus_tag="PKJ16_00375"
     CDS             complement(16411..17208)
                     /locus_tag="PKJ16_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV45464.1"
                     /translation="MKDVSRINLAPFDRPEVLSNLFWPRQDDSASQVPGAIDLVIPVD
                     NGERVGGRLYVKDRAAPSILFFHGNGEVAADYDDIAQLYLEAGLNFLPVDYRGYGRSS
                     GRPTVTGMLRDCHYCFDFFSRYCSENGFTGKRFVMGRSLGSASALELADACRDDIAGI
                     IIDSGFAYALPLLDLMGIDTAALGLTEEAGMRNIDKMQRFDKPTLIIHAQYDQIIPYS
                     DAKALYEACPAKDKRLLMIPGADHNTIFFHGTGEYMYAVMQLCLQPQ"
     gene            complement(17205..17750)
                     /locus_tag="PKJ16_00380"
     CDS             complement(17205..17750)
                     /locus_tag="PKJ16_00380"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV45465.1"
                     /translation="MKYTIETYRTLPESAPFELIRGDLVMVPAPNWKHQRISRRLEHA
                     LILHVEHEGLGEIFGAPVDVYFDDTNILQPDIVFIAKDRKSMLGDKWYIQGAPDLVIE
                     ILSPTNSSMDAIEKMAIYERFGVREHWIVDPELRTMHVHILENGRYRLVKQYCGDEPA
                     ASVILPGFSVMPATIFEDLPA"
     gene            complement(17901..18242)
                     /locus_tag="PKJ16_00385"
     CDS             complement(17901..18242)
                     /locus_tag="PKJ16_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV45466.1"
                     /translation="MKYIIAIIKPHRLEDVMKELDKEKVYLRTVVDVVGCGRQRGITE
                     VYRGVKEVESLLRKVKLEIAVNEDFVEPTIAAITRGAREGEIGDGKIFVFDLKECVRI
                     RTGERGKEAIG"
     gene            complement(18267..19502)
                     /locus_tag="PKJ16_00390"
     CDS             complement(18267..19502)
                     /locus_tag="PKJ16_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNV45467.1"
                     /translation="MPQLNNADIVWMLISTAMVMIMTPTLAFFYGGLVRKKNILSVLM
                     QCMVILCVISVQWVLFGYSLAFGPDVGGVIGALDWIGLRGVGLQPHDTYMSTIPHQLF
                     MAFQMMFAVITPALIIGAFVERIKFSAFLVFVILWATLIYDPLAHWVWAKDGWLAKIG
                     ALDFAGGTVIHINAGIAALVFALMLGKREGVGHSRAPHNLPLCVLGAAFLWFGWFGFN
                     AGSAGSAGTLAVTAFINTNTAAAAAGVSWMLLDWCYNKVPTMLGVATGLVAGLVAVTP
                     AAGFVTPVSALAIGAISSVVCFIFVTKVKPFFGYDDSLDVFGVHGMGGIVGALATGVF
                     ATKAVNPAGADGLLYGNPGQLWVQFIATIAAIAFSAIGTAVLYKFVDLVIGMRVDTRD
                     EYIGLDLTQHRETGYTVIE"
     gene            19796..21079
                     /locus_tag="PKJ16_00395"
     CDS             19796..21079
                     /locus_tag="PKJ16_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNV45468.1"
                     /translation="MPFSMRRVFARWALLIAVSAAAACSSTQLEYPLWNPYARDAIER
                     VIVENGKRAPNYTGDARPYIVLDWDNTSIMNDCAGNLFVYQIDRLAYKLTPAEFSRVI
                     RTGIPNLYRTFPAKHRNKAGAAVTPFQVFADVDAHYQKLAQLMKTMDAAEIKLTDVFQ
                     DFKAKMLFLYYALDDAFGADVSYKWAMYLFVNYTPDELRQFATESHIHNLDLPLRAVT
                     FASPPEMKTRAGAVSAVHRQGVRIQLGILDLVRACERNGVDVFICTASLDEIVIAFAS
                     NPLFEYNIPRENVIGMRLQTRDGRYMAAYQQNYAFNYRKGKRDNVSELIAKSRGGRGP
                     VIVGGDSDGDWEMLSGFGDTKTGLILNKYREGNGIAVGAFNAARAQRMKEPAPRYVLQ
                     GRNENTGEWAPRMEVLLLGDEDDEDNYKLIQLPKK"
     gene            21104..21406
                     /locus_tag="PKJ16_00400"
     CDS             21104..21406
                     /locus_tag="PKJ16_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45469.1"
                     /translation="MIDLDHMPAAVKWAALVLATGFVAQFGKMLAEYVVKRYRERHAS
                     SNTPQAARPSGETTGGAERRAASSPFEGAAVPPVDSKIEKKMRKARYKANKKSRDR"
     gene            complement(21424..22518)
                     /locus_tag="PKJ16_00405"
     CDS             complement(21424..22518)
                     /locus_tag="PKJ16_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HNV45470.1"
                     /translation="MGDTERLVQKNEEVVDVSKLSPEDMKKVQEIAKQIDIDDSQAVV
                     TYGVGAQREISSFSDTILNEVRTKDTGFVGDILTNLVVNIKDLDVDSLSTGGSFVSKI
                     PVIGQLVDSVKRFIIRYEKTSLQIEKITDELDKARMQLLKDIALLDNMYEKNQEYLKN
                     LDLYIAAGQIKLKELQEKTIPEMKAKADASNDPADAQKFQDLAMAVNRFEKKVHDMKL
                     SRMIAIQTAPQVRMIQGSNQTLVEKIQTSILNTIPLWKNQIVIAITLFRQNKALKLQK
                     EITKTTNDLLSKNAELLKQSSIDIAKETERGIVDIETLKKVNNDLITTIEETLKIQAE
                     GRVKRAQAEKELIKLETDLKQKLTTVKESK"
     gene            complement(22623..23291)
                     /locus_tag="PKJ16_00410"
     CDS             complement(22623..23291)
                     /locus_tag="PKJ16_00410"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HNV45471.1"
                     /translation="MTQSPKQKSSGWPRQLISLAIAGGVFAGLHVFLNIGIFVSLLAA
                     LGGYIAGLIIFRRRARPEGVEILSGGVSQSDLRATLAEGSRKLNEMRSYGMRIRNAEV
                     KRKVYDICAVVERIYDNFKKDPKDIKAAKQFLSYYFDAVINILKRYVELSSQPVQSAE
                     VKASLEKAEGILDSIHRAFEAQYDKLLRDDVLDLETEIKVLKNMITMEGLGGSAPSTE
                     EKQS"
     gene            complement(23358..24416)
                     /locus_tag="PKJ16_00415"
     CDS             complement(23358..24416)
                     /locus_tag="PKJ16_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV45472.1"
                     /translation="MKQAVVIGGGVVGCHIALELAERRYEVFLLERHSRLGMDTSTRN
                     SGVLHAGIYYPADSLKARLCVEGNRLSREFFDRTGVQYRPSGKFIIARDRSEISEIEA
                     LRDKAIANGAEGISLVPQDTVQKRVPCVRCVGALHSASTCIIDTGDYLTTLKTLLMQR
                     DVHLLYECPALAIDGGVRVETPRGSVDADIVINAAGLYADEVARMTGLDDFTVVPLKG
                     DYYCTTALPLEVPVYPPPNHGHGTLGVHLTPTCGPDVIIGPSETPSRGKDNYDIETPF
                     AVFDDSLRDMIPNYEAIRPSLREGFSGNRPRAFRGGTRCEDFIIMKQPDSVIHLLSIE
                     SPGLTSAPAIARFVVKMI"
     gene            complement(24442..24900)
                     /locus_tag="PKJ16_00420"
     CDS             complement(24442..24900)
                     /locus_tag="PKJ16_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45473.1"
                     /translation="MIKTTINIHVDTLGRVSAVAGALNMPRRDIIVALLMRIMKHNAR
                     FRRGFSTVTYQPDDPRGVWRCFSISFKKDENEFFVDLRKVCKRSVSYLVAIAVEEYLD
                     DFVAEHINGMHNYAPFRHYVLRQEVIDDVICWHCYWGNPYRCQKTTQTRL"
     gene            complement(24972..25655)
                     /locus_tag="PKJ16_00425"
     CDS             complement(24972..25655)
                     /locus_tag="PKJ16_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNV45474.1"
                     /translation="MNASWYDDSGAVINYYDIFNLPYTARADEVRTAFCALIKRYHTD
                     CTGMNSDTDRKKIALIISAYKLLSDRESRARYDRALFPHGDDGAPSYPIIPRSRVKYS
                     FSLADLLRNRLLNPRMKRRDYIHSLGQDVEILVTSREAARGAIALVELSSRSQCPLCL
                     GRDRECNVCRGVGRIQTTNALQVAIRPGTPDGTVMDFDLMALKPDRFTSFTMRSVRIK
                     ITVGAASAR"
     gene            complement(25652..26572)
                     /locus_tag="PKJ16_00430"
     CDS             complement(25652..26572)
                     /locus_tag="PKJ16_00430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV45475.1"
                     /translation="MKILIVKPGGIGDTLLMFPALVELGVRFPGAQFDMIGNRAAQEL
                     FRISGRIANARSIDDRLSTRLLCAADREAVDILGSYDRLFFLIDDGDGEIAHRLTAAG
                     IRDFVIASSRQADPRVPQWRYCIEICGLRQTLSEDDYCREFTRGFPPPAQRGGGVIIH
                     PGSGSATKNWPPERFCAVIDYCCSNLREPIALVMGYAENGLHAAFERMRHHPRISFFV
                     DCPLAEVVSLLRGARTFLGNDSGIAHVAGLLGVPSVVLFGPTDPAAWKPIGGSVTVLA
                     PPDGERSSNGIATDAVIAALHCALGMEYGP"
     gene            26626..27324
                     /locus_tag="PKJ16_00435"
     CDS             26626..27324
                     /locus_tag="PKJ16_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45476.1"
                     /translation="MSHERNIILCQPDATKSCAACCGVFNLRDITEDALARYCGDYEV
                     RARRAYRLAPGCHLEGAADLEKRDIGAHVCPYAGFAHPGRPGCMLHPAVRGREERDRS
                     LFGAKICAEFLCPAHAILTDAEKKLLVGALTHWYPYTVAIVDPESFRWIIERVAEGYA
                     CSPAEIMSHQAARGSITAALRAHADYLNGIDGPIFQYSVAEYALGRSRFSLAGGADAL
                     ARHRARVLDSIAIK"
     gene            27392..28234
                     /locus_tag="PKJ16_00440"
     CDS             27392..28234
                     /locus_tag="PKJ16_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNV45477.1"
                     /translation="MECFGIVGAGMMGTDIAHLALSSGFRVVVCDCDAAQLDRARETL
                     GARFRRYVAEGRFRQVEMDGLLSRLITERSIGALAECDMVLECVTEDRSVKMEVFRAL
                     DGAVKGGAILATNTSSISITRIASVTKRPESVIGMHFMYPARVMKLVELVCGLATTRE
                     TFESAKAIVRQFGKQFVESQDSPGFLLNRIFAPMINEAVFALYEGAGTAESIDKAMKL
                     GTNVPLGPLELADMMGLDVVLAVIEVMFDEFGDPKYRPCPLLKKYVAAGYLGKKSGRG
                     FYTY"
     gene            complement(28228..29112)
                     /locus_tag="PKJ16_00445"
     CDS             complement(28228..29112)
                     /locus_tag="PKJ16_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45478.1"
                     /translation="MKNSSGAAGQQLSREIGFLARKVINDDFSREMVRREIESRFTTA
                     TRSDRERILGQVVQRCYTAIWLAMPAPRKHARTSTTRTRAAAAAKTLPVTVSLAQETF
                     ALRAARIITELVADDPGRYACETGYRSILFDAALDRLVDQTLFDDCDCDPELAHIYLK
                     QTALILKKSAPQENAADWYRSLFLSFWNLVKWERLFPSMPEAAGMLQLNRGIAADIVM
                     KSSGAFHLAETAGEIIEMTGMGRRDNLMLVSFIDFSLFTWLRHFGIMDYCAGSRTAAM
                     RARLTDHGRAFLGSLQNQ"
     gene            29375..30304
                     /locus_tag="PKJ16_00450"
     CDS             29375..30304
                     /locus_tag="PKJ16_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HNV45479.1"
                     /translation="MSIIDRYRNHQDVKEIRKNAERILHRLSVEIGERTLRRYDRLEE
                     TRAFITDRLSATGHAYAEEYTVEGKTVANIVAEIPGTERPNEIIVIGAHYDTVEGTPG
                     ADDNGSAVTGLLEMFRLLSARAYRRTLRFVAFTLEEPPYFLTKDMGSMRYAKGCRERK
                     ELIRFMVCLEMIGYAGRSIEQQYPIREMKERFPRIGDFLTVVSLPSSSPYTYLWKRVY
                     NQHAKKAVHELIGPASIPGIDFSDHCSFCRNGFPAIMLTDTAFYRNKAYHTEHDTYDT
                     INFDFLADVIRNSARTLAEIADMDELPVAECGC"
     gene            30424..30783
                     /locus_tag="PKJ16_00455"
     CDS             30424..30783
                     /locus_tag="PKJ16_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004469820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV45480.1"
                     /translation="MAEKSDRQARLAEDNIVNSIIGEGSEFKGEFKVNGLLRIDGKFK
                     GTIETEGKVLIGKSGEAVTDIKASIVIIGGLVEGNIFAGERVVMLSSGQVRGNVITPS
                     LVMEDGVVFEGNCVINS"
     gene            30802..31248
                     /locus_tag="PKJ16_00460"
     CDS             30802..31248
                     /locus_tag="PKJ16_00460"
                     /inference="COORDINATES: protein motif:HMM:NF015822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HNV45481.1"
                     /translation="MIEIIANHPNKDERTKVKGKKRSGAPAGTARAFREPLQQAVAAE
                     VEGTIDDLMGNLAEQEKRFLDQQNLYEMGRYKALVQKILKMVLDEGYTTAVLKRHSRN
                     KADFIIVDRINARLADISLHITRGTSGFNLLKTMEEIRGLVLDLLS"
     gene            31331..32377
                     /locus_tag="PKJ16_00465"
     CDS             31331..32377
                     /locus_tag="PKJ16_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45482.1"
                     /translation="MLTFSDILGHDEPLRRLAGLRDRNAIPNTLLFSGPEGIGKRLVA
                     MAFLASLFCRGDTRPCGACAPCRQIAADTFPDLVMLLPNDKGVIPIGNAEKREPGSVR
                     WLVDRLARRSVSGRAAVLIDGIDRATDEGQNALLKSIEEPGEGAVIVLVTADRSRVLP
                     TIRSRCFELAFRPLDDQAIMRHLSRGGVDEDDARFIAHLAQGSFSAAGLLMEGTTCEE
                     CLALAGEISAFLIRGELLTLDLGGLQKRLSAEAIVDTLTAIYRLNMRVLTGARDVAPA
                     KEARGALYPVFYERVCIDNLSMVTALVKIFLALRAGLRYNLNLRNSLKGSLYRAREGA
                     AGDDASPDTLPERG"
     gene            32381..33244
                     /gene="ricT"
                     /locus_tag="PKJ16_00470"
     CDS             32381..33244
                     /gene="ricT"
                     /locus_tag="PKJ16_00470"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HNV45483.1"
                     /translation="MDVALIRLRNTSQISCVNTNNLFIKSGTHCVIEMDNGVDLGQVL
                     KRQSSVKTNGAEVKGKLLRKATPDDFKRVPELEALENRAFAVCRDKARERGLGMRLVS
                     VKCLFDKTKIIFYFVAENRIDFRELVRDLASAFKTRIEMRQIGVRDKAKLMGGFGPCG
                     RELCCSCLKEEFEPVSIKMAKEQNLNLNSLKISGMCGRLLCCLWHEYDVYRELNRDLP
                     SVGNEVRTEKKTYTVDSVDTLKQQIRLRSRDCVLCVAKRDLTRAGDHYTVSEELIQQK
                     IKQFEDDENAE"
     gene            33262..35181
                     /gene="metG"
                     /locus_tag="PKJ16_00475"
     CDS             33262..35181
                     /gene="metG"
                     /locus_tag="PKJ16_00475"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018123220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HNV45484.1"
                     /translation="MHNRFYVTTPIYYVNAEPHIGHLYTTVLADFLTRFYRLRGYDAF
                     FLTGTDEHGDKIARAAAERGREPQEYADRISAVFRETWRDIGIGFDDFIRTSEPRHRR
                     VVQAILQRVYDAGDIYFSSYGGHYCVGCERFFTEKEMVDGKCPDHDRPLEFVQEENYF
                     FRMSKYQGWLAEHINTHPDFIRPERYRNEVLSILNNEVLEDLCISRPKTRLQWGIPLP
                     FDERYVTYVWFDALINYISALGFPDDSRFKTYWPAAQHLIAKDIVKPHGIFWPTMLKA
                     AGIEPYRNLNVHGYWKMEDAKMSKSLGNVVGPRDLIARFGNDQIRYFFLREMTFGLDA
                     KFTEEAVINRINYDLANDLGNLVKRSFAMTQKYFAGRVPARSSADDAGRGELAALLKA
                     SADEYSAAVERCQFSVGLERLWEFVRALNRYIDERKPWALAKEPGSPALASVMRNLLE
                     SIHALAVLLAPVCVDGSAKILAGLGRGDTVVAIDTLFSLDCLADGQAITDPGILYPRI
                     EREETPAVSPEKENKPVNTEQDGLLDIGDFAKVDIRVAKVLTAERIEGSDKLLELRVD
                     SGLDERTIIAGIAQHYEPAYLVGKKILLVANLKPAVLFKRTSNGMLLAAKKDKKDAPV
                     LIEVDDRIPVGARLS"
     gene            35188..35685
                     /locus_tag="PKJ16_00480"
     CDS             35188..35685
                     /locus_tag="PKJ16_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HNV45485.1"
                     /translation="MKIGIISDTHNNIEITLKAVAVFKERSVEMVLHGGDLTSPKMME
                     FFKSLSCDCRFVLGNSDIDSDLLNDRAQRLGFGCVEDTCDITVGGKRILVFHGDNVPL
                     FRKAVASGEYDYIIKGHTHFFENYVSNNTRVINPGSLFGDDEHTVAVLDTETDRVEKI
                     RIEME"
     gene            35900..36280
                     /locus_tag="PKJ16_00485"
     CDS             35900..36280
                     /locus_tag="PKJ16_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020605821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HNV45486.1"
                     /translation="MKIIMTPDAPKPGGHYAHAVIENDIVYVSGQLPLDPTTGKLKGN
                     SIEEQTEQCLANVAAILKAAGSRPDLVLKVTVYLSKTIWLGKVNDIYARFFGAHTPAR
                     VVVPIEDLPNAMWIEIDAIAAVAK"
     gene            complement(36367..37635)
                     /locus_tag="PKJ16_00490"
     CDS             complement(36367..37635)
                     /locus_tag="PKJ16_00490"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV45487.1"
                     /translation="MSELFEKNSRNIWKPFILNIMLVILLFIVGIFIGIFFKNKDLIE
                     SGMLSNARAHFRNIVITRSWNARYGGVYVEKTKGVESNPYLKNPDIHGINGKTYTKKN
                     PALMTREISQIAERDGLFRFHITSLMPLNPGNKADPFEEKALRSFERGTKETSTVVRS
                     ADRYLFRYMAPLVVDRSCLTCHAEQGYRIGDIRGGISVSFDITDTRRQINLYMVVIII
                     LGVATFAIIFLIIYLLIRKLIRRITEAQVLIEEMATHDELTGLYNRRYFFTRYREECD
                     RAIRYRKNLCIMMIDIDHFKAINDTHGHQIGDVALKNLSELLMRNTRASDIAARYGGE
                     EFVVMIPEAGRDGVMLFAEKIRSLVESSSTMSETGADIRFTISIGIVIVGASELAGIA
                     NTDTVIKFADDALYRAKRNGRNRIEIDERP"
     gene            complement(37798..38727)
                     /locus_tag="PKJ16_00495"
     CDS             complement(37798..38727)
                     /locus_tag="PKJ16_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HNV45488.1"
                     /translation="MAISSLDVDVLVIGRSCVDYLYVVDSYPPEDRKIAVAEHLVEAG
                     GQGSTAACCVARLGGRVRFIGSVGDDDAGRFCLDRLADFGVPTDLVKIIPGGRTPTAA
                     IVIAGGGSRTIFYEPSALPKPVVDDLLMSHAAAAPVILLDPQATYLAPMLGAAQHGLI
                     VYDCERRREGLEDMMAAADYFIPSAEFFLTDSDGGAEITLFDRALDFRTHVTGELIVT
                     DGPRGAAWFTGKEIITAAPPPETVKDTTGAGDNFHAAFALGLARGLPREELLAFAVAV
                     ATLSCRAYGGRRGIPSWDEALSASRRCDVRHIL"
     gene            38901..39242
                     /locus_tag="PKJ16_00500"
     CDS             38901..39242
                     /locus_tag="PKJ16_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45489.1"
                     /translation="MSSLKSRLTAILNLDTRNQPYSATQIAGMMAIVVLLFPLLFTMF
                     GFLSAPLAGLIEVVFRPRDPDAVRAAKIAVLIVKTALGLTGGLWICRIVWPKRLALPR
                     DQGDEHPVFPK"
     gene            39343..40017
                     /locus_tag="PKJ16_00505"
     CDS             39343..40017
                     /locus_tag="PKJ16_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV45490.1"
                     /translation="MYSTGDILNARAKPSMSAPIVVQIPYGSIVNVAKTDVRMTYEGM
                     TYHWYRVKEADAYVLGAFLSVKRPSAKKEMKLCLWGENEGCPAQSAVVRLSGMKASSS
                     RYQGCHSEEYRSELSGDYAIRKGYLEIVWKDYETIRTVIAPGRARSSTRVRNKGYIQR
                     FHWRADYAGWIDDDTLALMQKRKCRLDPKNCFLDCTDPSITDAAEREMDANENIRSFY
                     CVKECK"
     gene            complement(40059..40925)
                     /locus_tag="PKJ16_00510"
     CDS             complement(40059..40925)
                     /locus_tag="PKJ16_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNV45491.1"
                     /translation="MAKKALGKGLEAIISKSTTPVADFEEQVIAHGDRIVHIDTNAIK
                     PNPDQPRTHFDENEIKGLAESIRSVGMLQPIIVRRAGSDYFVVAGERRLRASKLAGVP
                     KIRAIVIQASEEENITLALVENIQRANLDPIEEAKVYRVLSERFSLTQNDIAGRVGKD
                     RATIANLLRLLALPSEIQASISAGELSVGHAKVLLGTSGAQQKTLFREIIDNGLSVRA
                     LESRLAGSAGTKKPSPAKPKSKNPQIRKMEELLVSRLGTKVEIKHAGPHGTIEISYYS
                     LDDFDRIVELIT"
     gene            complement(40918..41679)
                     /locus_tag="PKJ16_00515"
     CDS             complement(40918..41679)
                     /locus_tag="PKJ16_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004607099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV45492.1"
                     /translation="MGRIISVSNQKGGVGKTTTTVNIAAFIAEKGRRVLIVDIDPQAN
                     AGYGLGVRVEEITSTVYEVLTGEKTIAQCVQPTSVPNLSIVPSNIHLSGAQVELLDLE
                     GKEFTLRNAMQAIRDEYDYIFIDCPPSLGILTLNSLVAADSVLIPLQCEYYALEGLSQ
                     LLRIIAMVQERLNPRLAIEGVVLTMYDPRTNLAQQVVSDVREFFKEKVFTTIIPRNVR
                     LSEAPSFGKPIGMYDRHSTGSDSYEKLALEVLANG"
     gene            complement(41700..42890)
                     /locus_tag="PKJ16_00520"
     CDS             complement(41700..42890)
                     /locus_tag="PKJ16_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45493.1"
                     /translation="MRTVAVIALMILPLFSQTARDATRANADQPAGLVEGKFENAGAA
                     LRQLSIPFERIRLKDLERPARTYRVLFFPCGIGPAIEDAIDVRSHGIRLNAVTLTDDH
                     YSVNRARVCGSIRQYVENGGAAYFSDLSYEYAQCAFSMFSFFDGFPNVGRAGTVELTH
                     HGDLLAFTRLRHDTRTVLHSGWVAPESISGAVPLATGEFSTPRGERSGPVAALISRGK
                     GTIAFSSHHEDRITELFRFLAYRLYHRDLVASLEERARENGQTVRVRIADRVLAGEAV
                     RAYLVPLQSGMNTLYVSCAGDELSLDVFSSDSLLAAYSLDQREFLVDIPSDGDRTVRV
                     HLYPSRGVRATPYALLAASGRRARPHVRYALYGAAAVACLAILILAIKTLFFTKRFRG
                     RVRR"
     gene            complement(42897..44846)
                     /locus_tag="PKJ16_00525"
     CDS             complement(42897..44846)
                     /locus_tag="PKJ16_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45494.1"
                     /translation="MNPQDIKKLERRAKSLLLDKDIPPAKMDLVRSLINNGAISPDEK
                     YRTIIDLIQSCPDKPRGAPPGERVEIKKPQVLRKKPVPAKKSEPEKPASAPEPSDLTR
                     KAIPTLYDKYREYKLFKKRLLIHANNRFGIGFRKRLVPLPRYFAVFREIAACQETVTV
                     LLQRVMEALLADPAVEDPTHFNYLRVLARWMLIAPFSQVPPDRAKWMDRSVFEPELKD
                     YAIYFFSFLRLDGETREQLFLLVESRLRELDGLRKEPFDERDTDAVRRDKEKKNLSSE
                     KTIHEFIGLIRSFLPATIGAESALSALLKKKYGLPSFPDFLLASLEALVFEREVAPAD
                     IVEHYAIGAPTVRTDSWDYSADYLKKIGKDPETKRRRYAEQLRRELVYYEEVNSLLQL
                     GEGASLLLQSAFEHQWRHIDKKKGDPGTQHEENFFGFMEGCLAFFAGVILPIIAGARA
                     TLQDARGETRDEALFGPAYFEKERALFLELQGEIHYFKSNNPTLVVTRTQVERILSGE
                     TKSLAHLQHYLGRLASVFYQIGSECHRLLRTHQRWRASDASSDGPDDARTPLLSRSAV
                     DDTDEAGRQFPFFDCTIKGFPGRAQASKYLVAREVVGDLANPGFLLVAIAFCYQLAFA
                     CKESSLYRLLDERRSIIKRIRDLTS"
     gene            complement(44971..45987)
                     /locus_tag="PKJ16_00530"
     CDS             complement(44971..45987)
                     /locus_tag="PKJ16_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45495.1"
                     /translation="MRMKFKVLVLIALAAILAIGSTNVFADAPRARVQDMHRTWEMLD
                     LKFMLSPEFGLFTQPNVRTEHYRRDTYDPKTAQTSHNGQPLGVWFLELYTGFLYNLKV
                     DDSLKVTFGADWYFNGSPQSDSKNIHNTRNNYPYEHAINVWAVPSYTIGDFTIMARII
                     LYNQIFEGNTETWVLRTNADGTDGGSYRRGWDKSEEYGWAMTTRALVGLEYKLTKTAT
                     IFARYEIFYGTIADNETRFVPDNEQSEDPNKTTERNRDNNESRMAYGDGGFAKIGYYR
                     NRAYLGYSLKLASNVSFINQYVCETNLNPNFDPDGLTLADRTQVTSIVHYVFIGASLT
                     FNLY"
     gene            complement(46236..47981)
                     /locus_tag="PKJ16_00535"
     CDS             complement(46236..47981)
                     /locus_tag="PKJ16_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HNV45496.1"
                     /translation="MNVLLILVITVAVFFIGYRFYGRFIEKVFDVDGSHATPAHEQRD
                     DIDFVPTKKFVLFGHHFASIAGGGPILGPTFALIFGYLPVWLWIVVGTFLIGAVHDFT
                     ALLASVRENGRSMAEIAEKTLGRTGFMLFIGFTVIMLLMVTSVFLTATAKALSSMYPM
                     SHFPSDLAQGVRTVDVNGVPHAVIGGIASTSVIVISCCAPLLGYLLYKRGISVLLGSV
                     IAITVCVVSVLVGFLHPISINPFVWQIILSIYTIFAAGIPVWLVLQPRDFTNSFLLYG
                     GVVLMIAATVVIGISGGTIAAPANNIAAAAAMSEVGWVWPLLFITVACGAISGFHALV
                     AGGTSSKQLENERPDARYVGYGGMVLEGLLAMGVVIAVGYGLKFDEFVGIVHPADPTK
                     SNPILAFALGLGGMLNSAFGMPKIIGTIFGILMVEGFVVTTLDTGVRLNRYLLEELWR
                     AIFPRVPKLLNTYLFNSLLCVALMFLLAYFNAFGALWKLFGSANQLLAALTLITVSMW
                     LLQRGKRYMFAFIPGIFMFATTIAALIYLLVFSYIPSGNIALTAGDIMLILLAIGVVV
                     LVIRFLLERRRSARA"
     gene            complement(48369..49511)
                     /gene="rlmD"
                     /locus_tag="PKJ16_00540"
     CDS             complement(48369..49511)
                     /gene="rlmD"
                     /locus_tag="PKJ16_00540"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HNV45497.1"
                     /translation="MKKKKRAFLELARRYADVAVDPSCPHAGECGGCALQHLPYESQL
                     SLKRDHLAALFDGAIDVGPVQPADPFGYRNRMDFVTAFGKIGLRRAGSFKHVVDIGSC
                     RIQQDAADEAFRRVRELLAGVEDYDYLAHAGYLRYVVIRQGRFTGEVMLNFVVASPDG
                     RGRLDPVIAAMADRVASISILLSAGRADLSFGEILEHAKGGAIDERLDDLRFAIAPNA
                     FFQSNSEIALRMYRRIREHARGHVLDLYAGIGSITLFIAGACETVTGVEIVEEAVTAA
                     RANAVQNGIANASFVHGDAADFLATFTGRCTTLVLDPPRAGMHPAAVKRIAALAPERI
                     VYMSCNPESFKDDLLRLDGYRLTSFEAYDMFPQTPHIETLAVLDRR"
     gene            49700..50671
                     /gene="rfaD"
                     /locus_tag="PKJ16_00545"
     CDS             49700..50671
                     /gene="rfaD"
                     /locus_tag="PKJ16_00545"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014322382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HNV45498.1"
                     /translation="MIIVTGGAGFIGSAFLWKLNEQGIDDVLVVDRLGTSDKWKNLVH
                     RRCIDFIHKDDFPGLLDDGNRYFRASAIIHMGACSATTERDADYLYANNYRYTRTLAE
                     WCLKRNVFFMYASSAATYGDGARGFSDDPAGMDALTPLNMYAFSKQLFDQWALRNRVL
                     DRMTGIKFFNVYGPNEYHKAHMVSMIYRAFHQIRETGRVRLFKSYLQQYADGGQLRDF
                     VYIKDCVDVMWWLFNNRSASGIYNLGTGTARSWNDLAGAIFAAMGKKTDIEYIEMPEE
                     LRDRYQYFTEADMARLRAAGCNHTFMKMEDSVRDYVVNYLARDVRCL"
     gene            50675..51343
                     /locus_tag="PKJ16_00550"
     CDS             50675..51343
                     /locus_tag="PKJ16_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV45499.1"
                     /translation="MKRFLFSYVGPYVGLAILKSLTATYRIRIVDEENEQSIYRRGSR
                     PIYCSWHQRFFPGIAFFPKRNKIAIMISPSADGELIARVVKILGWHPSRGSSSRRGKE
                     ALMELRRLAAERYSIGHIVDGPKGPFGEVKPGLISMAQHSGLPILPTITSAERRWVFG
                     SWDRFMVPKPFSRIIIRFGKEIPVPSEIGDAQFEELRRQVERTLRELYEDTDRMWERA
                     PQAS"
     gene            51419..51898
                     /locus_tag="PKJ16_00555"
     CDS             51419..51898
                     /locus_tag="PKJ16_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV45500.1"
                     /translation="MSTLINIGVAQVGTASSPAVLRTILGSCIGVCVYERMKKMGGMA
                     HVLLPSTVQGGKPEKYADTAIPMLVERLLADGCKKEFMSAKIAGGASMFTFNSSVALA
                     QIGERNAEMARAVLAKYGIPIVIDDTGGNAGRVIDFFLEDGRLKVKSAGEEKIYYKL"
     gene            51926..52786
                     /locus_tag="PKJ16_00560"
     CDS             51926..52786
                     /locus_tag="PKJ16_00560"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV45501.1"
                     /translation="MNPQIKQRIESVINNIEQLPSIPEVATRIINMVNDPEVSFKAIA
                     DEISKDQMITTNILKLCNSAYFSKGKEISSVDRAIVTLGLKEVKDAVILAATKIVLNK
                     IIMGYDLAKGELWRHGLYVAVLAKKIALMKGKKDIADVAFTGGLIHDVGKTILALFVQ
                     GTFKEIMRTVEEKKIPFIAAEKDIMGFDHQEIGERILSKWKFPEVLKASVRHHHQPMS
                     APDEFRTVVSIVHVANSLCLMAGVGIGSDGLYHELVPEAIELTKLEPGELEMLFAGIP
                     EMGEQAETLI"
     gene            52795..53184
                     /locus_tag="PKJ16_00565"
     CDS             52795..53184
                     /locus_tag="PKJ16_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45502.1"
                     /translation="MHIVKRCPACGKNLRFPVDKGKIRVTCVCGNAFTADPDDPRLFD
                     GAAFDMGAQKKPASRPAASVRVRALGDAVIQGLYSFTYRVQNFRLLPTREKAQVLLVV
                     VSVAALVAAVLIYFLVNPAPRPNSSVI"
     gene            53187..54119
                     /locus_tag="PKJ16_00570"
     CDS             53187..54119
                     /locus_tag="PKJ16_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45503.1"
                     /translation="MRLNTTDGYYAREIESLGWELTVCNMLARPESPCRGVLRVNASY
                     GALLADFLATLVPLASCSSVLEVGGGYGVLMRDFLDRVPHLACTMLDISPFLLERQHE
                     ALRGRSASFVARDFFSVEDACLAGFDCAILNEIVGDFPTACDIDPDALSNGTDADDPA
                     TARVRRIMLRYGIAPPEGARFNFNLGAAEAAERLCAAGVPYIFMSEHSCEARVPDALA
                     GLVSIAAPGNPERIPLKGHDEYTIAFSHLVAIAVGHGYRVVRGPLADIVPVDFDGRVR
                     FIMSSNASRSDEHEIIRQFVEDLFKYEYVVFVKG"
     gene            complement(54120..54602)
                     /locus_tag="PKJ16_00575"
     CDS             complement(54120..54602)
                     /locus_tag="PKJ16_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45504.1"
                     /translation="MMSDTLNRIMESGYPQGSGPQAKYVFSCPTCGAQVISQNSYGAA
                     ASSGTLQNILSTGAQQGLSRLLWRIPIIGSVLASVLGGLVSTHLGQRQAQNMQQQQDG
                     ARRQAFDEVRSRFAPCTTCGALTCASCSDDGLCRACAEKAKGGTQDAGAPKDPTKMWD
                     "
     gene            54773..>56844
                     /locus_tag="PKJ16_00580"
     CDS             54773..>56844
                     /locus_tag="PKJ16_00580"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV45505.1"
                     /translation="MAQKKKEKKLTPSTNEFQGTDVESLQVSFVNHLEYSCAKDEFSA
                     TKHDCYTSTALMTRDRLIERWIETQQSYYAADAKRVYYLSLEFLMGRTLGNSLINLGL
                     YDNAHRALVDLGHDLEELREIEWDAGLGNGGLGRLAACFMDSMATLELPAYGYGIRYE
                     YGIFFQSIVDGYQVETPDNWLRYGNPWEFPRAEYLYPVRFYGTVGHHADPSGAQRFEW
                     TDGQMVMAMAYDTPIPGYNNNTVNNIRLWSAKSTREFNLEYFNHGDYEKAVSDQANSE
                     TISKVLYPKDESFKGKELRLKQEYFFVSATLQDIIRRYKKIHDSFDAFPDKVAIQLND
                     THPAIAIAELMRILMDDEGMGWEAAWDITVKTFAYTNHTVLPEALEKWPVGLVESVLP
                     RHIMIIYEINRRFLEDIAARYPGDMNRLHRMSIIEEGAVKNVRMANLAIVGSHSVNGV
                     AALHTEIIKNNIFRDFFEYWPHKFNNKTNGITQRRWLKLCNPGLAGLITEAIGAGWTT
                     DLFELKKLEPLAGDAAFQAQWAAAKRVNKERLAEYIMRKNGVRVTVDSIFDCHIKRLH
                     EYKRQLLKVMHVVALYNRIKANPSAAITPRTVLFAGKSAPGYYMSKLIIKLINSVADV
                     VNNDPDVAGRLTVLFLVNYCVSNAEKIIPAADLSEQISTAGYEASGTGNMKFALNGAL
                     TVGTLDGA"
BASE COUNT        10901 a        17081 c        18231 g        10631 t
ORIGIN      
        1 actggtgaag gaacaaaagc tgagcgtttc gggttttatc gatccccggg aggcggtggc
       61 gatcggaaag ctctccggcg ccgacaccat cgtgaccggc acgggccacc tcgtctcgcg
      121 cgacagtgac gtgctgagct atctcgtcat caaggtcatc agcctgagat cggggtctgt
      181 gatcatgacg gcgaatctac ggaaaaacat gagcatttcg gacgccgtaa ccgagatgtc
      241 gcgctcgatc aaaaagtgtc tcgatgagcg cgcggcggcc cagaagggcg cacccaaata
      301 atcggcaccg ccgtcatgcg cttgcgctcc cgctctcacc gcgttttcct cgtcgatctt
      361 cgggatcgca ccgcaccgcc gttccctgcg gccgtgacca cggcatagga ttcgatgcgc
      421 gccatgcgaa cgatacggga cattctggca gcagaggcca cggtcgtttc gtttgtcgtg
      481 ttaaacgccg tcgccatctt catgacgccg ctcttcgccc acggttcgac gtgggagcgg
      541 gtcatctccc acgtcgattt cgtctgcgtg ctgtacttca tgctcgagtc ggcgatcaag
      601 atcaatcgcg acggctggcg cggctatatc gacgttggct ggaaccggtt cgatttcctc
      661 atcgtgctct gcagccttcc ggtcctgctg gagccgttcg tggacctgaa gggattcggg
      721 atcgtgatgg tgctccggct cggaaggctc ttccggctct tcagggtgat gcagttcatt
      781 cccaacctcg accgcatggc cgcggggatc gggcgcgcgc tgcgggcctc ggtcggcgtt
      841 ctcatggcga tcgtcatcgt caacatcatc ttcgccctgg gggggaccct cctgttcggc
      901 gagttcgcgc ccaagcattt cggcaatccg ctcatgtcca tgtacacgac cttcaaggtc
      961 ttcacggtgg aaggatggta cgagatcccc gacatgatcg cggccaatac cgacagcgac
     1021 ctcatggcgc tgggcgtacg cgtgtactat gtagtcgccg tcgtcgtcgg ggggctcatc
     1081 gggctttcgc tcgcgaacgc ggtgttcatc gacgagatga ccgccgacaa caacaagctc
     1141 ctcgaggaaa aaatcgacga gctctcgcgc gagctccgcg aactcaaggg gatgcttgcg
     1201 gaccgtgaac atccgcgcgg gtcgggacgg gcgcgcaggg ggccgcggac atgacggccg
     1261 agacggcgct tcacctcgtc ggcggggtcg ggctgtttct gctcggcatg accctgatga
     1321 ccgacggcct aaagaccctg gccggggatt cgctcaggcg cgccctcgcc cgattcaccg
     1381 gcgggcgcgt cacctcggtg atctccggcg ccggcgtgac cgccgtgatc cagtcgtcga
     1441 gcgccaccat cttcgcgacg atcggcttcg tgagcgccgg gttgatctcc ttcgaacagt
     1501 cgatcggcat catcctcggg gcgaacctcg ggaccacgag caccagctgg atcgtcacat
     1561 atctgggctt caagttcaag atcggcatcg tgtcctatcc gctcatcgcg ctcggggcgc
     1621 tcctcaagct cttcgccagc catcgctttt ccgcggccgg catcatcatg gcgggtttcg
     1681 gaatgatctt cgcggggatc gagatgatgc aggcggcgat ggcgtcggcg gcggtcttca
     1741 accttgccgg catcgggggc gccaccctcc ccgagcggat cgcgctcctg gggctcggcg
     1801 tcgcgatgac ccttctgctg caatcgtcca gcgccgcaat cgcggtgacc ctggcggccc
     1861 tcggcgcggg gagcatcacc atcctacagg cggcggtgct cgccatcggg cagaacatcg
     1921 gcaccacggt catttcgtcc atcggcgcca tcggggccgc gacgcaggcc aagcgcaccg
     1981 ccctcgcgca cgttgcgttc aatctgatca ccggcgccag cgtgtttctg ctgctcccct
     2041 ggtttgtcgc cgcctccggc tgcatcgcgc ggctcatcgg gcgcggcgag gacatcatcg
     2101 ccctgtccgc ctttcacacg ctgttcaatg tcgccggggt tctcgcgatc cttccgttta
     2161 cgtcgccgtt cgcgcggctg atcgagcgga tgcttcccga gcggggcccg gcgctcaccc
     2221 gacacctcga cgcctcggtc ctcatggtgc cgcaggtcgc gctggagacc gtccggcgaa
     2281 cgttggccga tatcgccgcg tacgtgatcg ggcacctgct gtcgctcttt cagggctatg
     2341 gccagatggc ggcgatgccg gaaacgctgg acacggcgcg cagcgcgctc gatcggacgc
     2401 gctttttttt gcaggatctt cgctcgttca acaactcgcc gctcctgcac gagcggcacg
     2461 ccaatatcat tcacgcgctc gaccacctgt accggctcat cggcgcctgt cgcgaaacga
     2521 accacctgcg cggcgcgctc gccaacccgg agctccgcga gatcgcgctg catctagcgg
     2581 accggatgga gctcctcctc ggctggctgg agggcggaag cgggtccgat cccctcggcg
     2641 acctcgaaca ggcgtcgttc tccatcgcgg gcatccggaa ggagcagcgc aaggagatcc
     2701 tgaggagaac gtcattgggg gagatcgaca ccgaggccgc cttcggcatc atcgaatcga
     2761 tgcgatggct ggaccggatc gcgtttcaca tctggcgctc ggtggtccac ctcgaaaaca
     2821 tcacccgcgc gcacgatcgt tcggagatcg cggctgatcc ggacggctac gggcggtaga
     2881 ccacccgccg cttcgcaaac tcccgcaggc ttttcaatac cagatagtcg gtgatgccgc
     2941 tggcgcgcga gaggcgcgaa acggtgtccg cgagatcacg ctcggtgcgc gcgtggatca
     3001 tcgcgaagag cggatactcc caggtgtgcg gatagaccgt tcgcttgtac aggtgcgaga
     3061 tggcgggctc gtcgatgaag acgtcgatca cgtcgttcat cgcgcactca gggcgccaca
     3121 cggtcatcgc gtttgcagta tagcccgctt cgcggtgccg cacggtggcg gcgtaccggc
     3181 gcatcacgcc ctcgtcccgc agcgcgcgcg cgatgcccac gagcgcgccc tggtggatgg
     3241 tcccggcatt gagatcgatg agcgcgcgaa agggctcgtc ggcgtcgggg aggtcgatct
     3301 gcaggaggcg caccgcttcg cgctcatcgg ccgagagcgc gcggggcggg gcggggttga
     3361 tcgcgtgcgc gagcgtcgcg cccgccgcgc cgtcgccgat gcggagattg aggccgatct
     3421 tgtagagcat ctcggtcttg aggatgaggt gccgcgtcgc gcgcacgcgc tcggcgaggg
     3481 actcgacggt cttgtggagc gaggtggcgc ggtccaccgc gagcgtgaac cagatgttaa
     3541 agtgatgatc gcgcaggtag ttgtggctca cccccgggtg tccgttgatg atgcgcgcgg
     3601 cgtcatcggc atccgcaccg tcgacctcga aagcgaccag cgaagtttcg tagcccaggc
     3661 tttccgcatt gaagatgccc gcgatgatgc gtatgatccc ccgcgcgcgc aaactcgaaa
     3721 gcgtatcgcg aacctcgttc tcgtccgcgc cgatggccgc ggcgatctcg cggcagggtc
     3781 gctccgcgag agggaaatcg gattggattc gattgaggag ggccgattcg agcgcgctaa
     3841 aggccatgcc gctgtctccg ctgcgggaca tacacgcaat agggctcctc ggcgaaatgg
     3901 tcgccggtcg catggaaggc gcgcgcccgg catccggcgc acaccccgcg gtattcgcac
     3961 gcgccgcatt tgccggtgag attggacagg tcccggaggc cgacaagcaa cggcgagtcg
     4021 ttccagatgt ccgcgaaccg ggtctgggtc acgttgcccg cgacaagcgg cagatagccg
     4081 cagggctgca cgtcgccccg gtgcgagatg aaacacacgc ccgttccggc cagacagccg
     4141 cgcgtcatgg ccgccatgcc gttcgtctca aaggcgacgg agcggccctc cgccttcgcc
     4201 cgctggcgga cgatgcggta gtagtgcggc gcgcaggtcg ccttgaactc gagcgccgtc
     4261 tcgcgcgagc ggtcgtagaa ccagttgagg atgcgctcgt attcgtcggg cgagatcatg
     4321 tcggtatcgg cgatttcgac gccgcagccg acgggcacca gcatgaacag gtgcagcgcg
     4381 gcggcgccgc ggtcctccgc gaggcgaagg atctcctcga gttccgcggt gtttctgcgg
     4441 gtgaccgtgg tgttgaattg gaattcgacg cccgccgcgg cgagccgctc ggccccgcgg
     4501 agcgccagct cgaacgagcc cgggatcccg cgaaagccgt cgtgcgagtc cgccgtgctc
     4561 ccgtcgatgg agatgctcac gcgcatgatc cccgcgtcgc ggatggcgcg cgccgcggcc
     4621 tcgtcgatca gggtgccgtt ggtggccagg gccgtccgga gtccgcgccc gcgcgcgtgc
     4681 cgggcgatgg cgaagatgtc gtcgcggaag agcggctcgc cgccggtgag gaccatgatg
     4741 ggcttcgcga aggctgcgat gtcgtcgatg atgccggtga tcgcgccggt cgggagctcg
     4801 ccggagaagg cggcgtcgtc ggcctcggcg cggcagtggc ggcagcgcag gttgcagcgc
     4861 ttggtgagct cccagaagat gaggcgcggc ccggacgacg ccttcatgaa tcgaacaggt
     4921 cctggatgag ttttttcgcg gccgcccggt cgcggtggcg cggcccgtcc tcggcgcggg
     4981 cgcgcttgat cgcctggatc gggttgtgga gcgtcttggt catgatctgg cgggtgaggt
     5041 cctcgacgat ggcgaggtcc gcgtcggaaa ggtgcttgag ccggcgacgg cggtagcgct
     5101 cgaactcggc ccggcggatc tcgtcgaagc gctcctggat cgccgagatg agcggcaccg
     5161 tgtcgagccg ctcgtaccag accatgaaat cgtcggcgtc ggcctcgacg aactgccggg
     5221 ccacctccag ctccttcatg cggctcttca ggttttcgtc ggcgatcgcg cgcaggtcgt
     5281 cgacgttgta gagatagatg ccgtccagcg cgccgaccgc cggatcgacg ttgcgcggca
     5341 cgccgatgtc gatcacgaac agcgggttgt tccggcgtcg gcgggcgatc tcgcggccca
     5401 tggcctcggt gatcagatgc gcgtcggaac ccgatgaggc gatgatgatg tccgcgcgcg
     5461 ccgcggcctc ggtgatgtcc tccagcggga tgatgtgcgc gcggcggttg atctcctcgg
     5521 cgatccgctc ggcgttgtgc aacgagcggt tcgcaatggt gatctcgcgg atgtcgctct
     5581 tcgtgaagta cttgaggatg agctcgccca tgtcgcccgc gccgataagc aacgcgctgc
     5641 gccgcgagag gtcctcgaag atcttcttgg cgagctcggc ggcgatgaag gcgacggaga
     5701 gcgggttgtg cgcgatctcg gtctccgtgc gcacccgttt cgcggtcttg aaggcctgat
     5761 ggaacaggcg gttgagcagc ggcccggtcc gcttcgccga gacggcgcag cggtaggcgt
     5821 ccttgacctg gcccacgatc tcgttttcgc cgatgaccat cgagtcgatc gacgaggcga
     5881 cggagaaaag atgcgcggcg gcgtcgcgcg agtatttccg gtacaaacag ggctccaggc
     5941 gacgacggtc gatgtcgatc cggtcgtcca ggagcgacag gaggccgtcg atcgcgcggg
     6001 ggatgtcaga cgcggcgaag tagagctcca cccggttgca ggtggacagg aagacgatct
     6061 cgtcgatgcc gcgctcccgg ctgcgcgcga gcaggtcgcg gacggcctcg tccggaagcg
     6121 agaggcgctc gcgcacgtcc accggcgcgg tcttgtggtt gaggccgatc acggcgatct
     6181 cggggatttt cgtttccgcg ctcatcttat tggaagctgt ggaatccggt ggtgatgaaa
     6241 aaattcacat acccaaaggc ggccgcgatc gacagaaaca aggcgatggt gatgcggttg
     6301 atgaggttcg tggagacgcc cgtccgcgcg cgcaacaggg ccaccgccag gatggccgcc
     6361 cacgagacgt agatgacgat ctccttgtgc gtgtacatcg acaccgtctc gtactggagc
     6421 cacagcaggg cgagtcccgt gacaagcccc gcgctgatga acgcgagcga gcgcagcgcc
     6481 gtccagcggt tgaagccgtc gatcgactcg agcgcgggca ggcggttgag gaagcgcatc
     6541 gaaccgcggc gcttgagctg gcgcgaggcg acgatcgagc acagcgatcc ggccgcgctc
     6601 aggaagaaga ggagctcgcc gaccacggtc aggacgatat ggagcggcag cagcccgtgc
     6661 gcggtgatga cggccatgct gcggtccgag ccgaagaagg ggaccgaggc gaacaggagc
     6721 atcgtgataa acggaacggc gatcgcgccg atgctgcgca cctgcgcccg ccaccggctg
     6781 aacggaaggt aggcgaggac gcaaatcatc accagggccg taaggaagaa gtaggcccag
     6841 atgccgtgga cggcgcgaag cagcgtctcc gcgctcaggg ggccgaagcg cacgatgccg
     6901 gtcgcgatca gcacggcgct ggccgtgtac agcgtccagc gcgcgagcgc gtgaacggcc
     6961 gcgcgatcgg tgaagaattg cgcgacgctc acgagcgagg cgaccgagaa aagcgccagc
     7021 gcgatgtaga acatgacgga gtgcatggct gcctggaccg gatcaaccgt ggaccggcgt
     7081 cgcggatgtg acaagcacct ttttatgccg catttttatt tgacgtttcc ccccgcatgg
     7141 gctatagtat atatgccgcc gaaaggcggt accttccaat taatccgcta caatgattga
     7201 cgattccgcc cggaatcgtc ctttttttgt gccggacggt tggatgaaaa acacttgcgc
     7261 tgtgcgcccg gcgcggtacg atgaacgcat gtttgatatc atggattatt tgccggacaa
     7321 cgatcccgcg ccgctggagg gccgctgcgt cgacgtcgcg ccccagtacg ccgacgacga
     7381 gatcgccgtc gagatcgatg cgctgttggc cgcgcggttc gatccgctct accggcgcga
     7441 caaattcaag gtcggcgccg caggcgtcga gatcatacat cccgccctcg cctatccggg
     7501 acggcgggcg ctcgtcaagg gaccggatta cgttcgcttc ctcctgtcgc tgtatccgca
     7561 gaagtccgac ctcgagagcg tcgagaagat cgtgctgcgc ccccgccatg tcgagctggg
     7621 cgatatcgag ctgatggcgc tgtacctgcg caatcggcgc atcctggtgc tctatctgta
     7681 cgtcccgcac ttctatgtcg tgggcgaatc gaagttccgc gagtacgcgg agttcgcccc
     7741 gtgcaagctc aacgacatcg tcggcggcag ccagacaggg cccggccgca tggccgcggc
     7801 ctcctcgacg gtgaaggtgc cgccgctctg gtatatcctg tccatgatat caccgtcccc
     7861 cgatccgggg gtggacaagt ttctcgtcaa gaaggaacgc gaacactacg agaagatctc
     7921 cgcaatgctc gacgaggttt cgtacttcta cgcccggcac ggctactgag cgatggccct
     7981 ggtcgattac catgtccaca ccccgctgtg cggccatgcc cacggcgagc cggcggccta
     8041 tgtggagcac gcgatcgccg tggggctctc cgagatcggc ttttcggacc acgcgccgct
     8101 ccccgcgggc atgcgggagg ggatcaccat gctcccggag gagacggagc gctacatcgc
     8161 catgatcgaa cgcgtgcgcg acgagcaccg ggggcgcatc ccggtgcgtc tcgggttcga
     8221 ggtcgattac ccgttgcacg acaccttcga ccggcgctat ctggacgatc cccgcatcga
     8281 ctacctcatc ggctcgtgcc acttcgtgga cggctgggcc ttcgaccacc ccgacggcgt
     8341 cgcggagtac aaccggcgcg acgtggacga ggtctacgcc gcctattacg atacgctcat
     8401 cgagctggcc aacgaggggc gcttccatat cgtgggccac ttcgacctgg tgaagaagtt
     8461 cgggcatcgc gcgacgcggg accatcacga ccgcatccgc cgggtgttga tggcgcttgc
     8521 gcggtgcggc accgccgtcg agatcagcac ggccggtctg cgcaagccgg tgggcgagat
     8581 ctatcccgcg cccgacatcg tcgcgctcct gttcgaaatg aacgtcccgg tcacgctggg
     8641 atcggacgcc catgcgccgg aggaggtcgg ctacgagttc gcccgggccc gagagctcat
     8701 tcgcggcgcg gggtatcggc gcatcgcgcg cttcgagaag cgggtgcgcc gcgatgtccc
     8761 gatctagcgc ggtccggcgc gtgcaagaaa tagttcttga cagttggaaa aatcgcccgc
     8821 agtattctcg acggtatccc gtgtgtgccc aatcccactc gccattatag gaggaatgat
     8881 gaagtacctc acgctgtgcg ccgttatcgt ccttgcgctc gcgctcgccg tgtcgtgcaa
     8941 gcaagcgccc aaggacatgt ccctcgatga tttcctgaag atccagaacg agatcatcaa
     9001 gaccgatctc acgcccgagg cgaaggacaa ggtcgtgacg aagttcggct acacgctcaa
     9061 gcagtacgac gacctcgacg agaaggtgaa gggcgatccg aagctgcagg agaagctcgg
     9121 cgagattcgg ctcaacatgg gcaagaacaa ggacatcaag aaagagttcg agtagaccga
     9181 agcggcgatg tgatcccgct cgcgctcact cgatgagtcc cagcacgcgg cgggcattgt
     9241 cgcgaaggat ggtgtcccgg tcgcgtgcgt cggtcagctt tttgatcggg gcgatatatc
     9301 gggcgaatcc cgtgagcggg tagtcgctcc ccaggatgat cttccccgaa ccgaccagcg
     9361 agaccgcgct ggcgtagatc cggttgtcgt agatgagcgg cgatgccgcg gtatcgtaat
     9421 acaccctcgc gaaggccttg cgcacctcgg gcatcaattc gtagaaaaga acgcccccgc
     9481 cccaatggga caggacgatc gtgaccgccg gatgatcggc gatgacatcg tagagcctac
     9541 cgaaatcgtt cgaaattttc cccgcatagc ggtgtccgag cggctcgttc acatgcaggc
     9601 acaccggcat cccgtgggtg tcggccgccg caagcacgga gcgcagatgc gacgcggccg
     9661 cctcggtgag tccgcttccg taatacgcga tctccccgat accgtacatg ccctgttccc
     9721 gcaggaggcg caccgcgccg tcgatctctt cggaggtcgt cgccgggtcc atgggcaccg
     9781 aaccgaaaaa gaagagcacg cctagctcga ttcgcgcgag ctcggcaaag tgatcgttct
     9841 gccgccgacg caggtcctcg tccgtccagg ggaagcccat caccaccgca ctgtttatgc
     9901 cggcgtcatc cattccggcc agcaggccgt cgtggccgac gatttttgcg cgcttgttcg
     9961 cgtagagcgc cgcgaaggcg ggatcggcca tgccggtctc gcgcgacgcg acgatctccg
    10021 gggtgaacag gtgcgtgtgg aaatcgatgg ccatgtcaat tcccggtccg ggccgctcgt
    10081 cgcaacgacg ggggcgtata acgcttgagg aggagcgaaa gcccaaccac cgtgaccgag
    10141 cttgccgcca tggcaaggcc cgcgagctcc ggtcggaacg tgattcccgc gaccggcgtc
    10201 aggacaccgg cggcgagcgg aacgagaagc aggttgtatg cgaaggccca gaagagattt
    10261 tgtttcacgc gccgcatcac cgccctgccg agctcgatcg cgcccgggat cccgaggggg
    10321 tcggcgccca ggagcacgat gtcgcccgtt tcgatggcca cgtcggtgcc gccgcccatg
    10381 gcgatgccca ggtccgcctg cgcgatggcc ggcgcgtcgt tgatgccgtc gcccgcgaag
    10441 gcaacgcggc cgccgcgttc ctgcagcgcg cggatgcggg cggccttgtt ctccggcagc
    10501 gcggacgagt agtgccgatc gatccccgcc tcggccgcga cggccgcgac ggcgtgtccc
    10561 ccgtcgccgc tgacgagaat cacctcgagc cccatcgagt ggagcgcgtc cacggccgcg
    10621 cgcgcgttca cgcgcagcgg atcgcgcacg gcgaaaaggc cgcgcaggac gccgtcgatc
    10681 gcgacgaaga ccagagaggc gccgtcgagc gcatggcgcg taatggcggt ggacgcggca
    10741 ttcggaatgg cgacgccgtt ttcgcggaga aacggttcgc tcccgaccag gacggtcctt
    10801 ccggccacgc gcgccgagat ccccttgccg ccgaatgcga caagcccctc ggccggatcg
    10861 ggcatgatgc cggatgccgc ggcatgcatg aggatggcgc tcgcgaaggg gtgcgtcgat
    10921 ttcgcctcgg ccccggccgc gagcgcgatg agctcgagtc gatcgatatc gaaggactcc
    10981 acggcgctca cctggggcgc tcccatggtg atggtgccgg tcttgtcgat catcactgcg
    11041 gtgagccgct ccgattgctc gagcgcctcg ccgcttttca caaggatgcc cagctcggcg
    11101 ccccggccga tccccacggt gagcgccgtg ggcgtggcca ggccgagcgc gcagggacag
    11161 gcgacgacga gtatggcgat gagcaccgac agggaaaaga tggccgtgcc gcccaggaga
    11221 aagaaccatg cggccgatgc ggtgagcgcg atggcgagaa tggttggaat gaaccatacg
    11281 atgatgcggt cggcgaagcg ttgcaccggc gggcgcgcgc cctgggcggt gcggaccagc
    11341 cggatgattc cggcaaggac ggtgtcgcgg ccgatccgct ccgcgcgcac cgtaagcagt
    11401 ccgtttccat tgatggttcc gccggtcacg cggtcacccg gcccacggtg gaccggaacc
    11461 ggctcgccgg tgagcatgga ctggtcgacg gcgctctctc cggcgatgac ggttccatca
    11521 acggggagcg cgtcgcccgg gcggacgaga acggtatcgc ccgcgcggac ggcgtcggcg
    11581 ggtacggtcg attcgacgcc gtcgcggatc acggtcgcac tctttgggtg caggtccatg
    11641 aggcggcgga tcgacgtcga tgtcttgcca cgcgcgcggg cctcgaggta ttttccaagc
    11701 atgagaaagg cggcgagcat gatcgcggtc tcgacgagca tgaactccat cgacaatacg
    11761 cccgtggcgg caagggcgct cgcgacgatg gccaccccca tgccgagcgc atacatcacg
    11821 tccatggtga gcgcgcggtt gcgcagcgca cggacggccc cggcgaagat gggggcgctg
    11881 acgaacagga acaccggcag cgccgccagg aagacgaacg gcgagtgcgc gatcatcttc
    11941 gcggcgggaa gatacatgag cgccatgagc ggaagcgcga cggcgagtcc cgcgacaaag
    12001 cggacctttc gggagcgaag ctcgcgcgcg gtctcctcgt cgtcgaagga cggcgcgtca
    12061 tcggcggtcc cgaggaagga gtacccggcg cgctcgaccg ccgccgcgat cgcgtcgatc
    12121 ggaaccggcg cgccggagaa atgcacgatg gcgatgccgg ttgcgtaatc gacctcggcc
    12181 gaggcgacgc cggggaggct cgccagagcg ccctcgatgg cgcgcgaaca catcacgcaa
    12241 ctcattccgc cgatagtgat cgtgacgctg ttcatgttct ccaccacccg cgcatcgata
    12301 tcatgggttg tcgcgccgtt gaaacgcgca tgcgcatcag ccccgtgatg cgaagtgtat
    12361 gccgcggatc cccgagtgac aagaaaaatt cgatgatttc atcgcagaat aactggacag
    12421 cgcttcgcgt cggttgatag gatgttgccc gggaggagcg tccatggtgc agatgaaatt
    12481 cgcgatcgcc cggtccgacg aggaggattt ctccgacgcg ctgttcgacg agggcgcgga
    12541 cagcgttgcc gtcgagaacg cccccgcgga cgatgcggtg gtgctgacgg ccattttccg
    12601 ggagatgaag acgctgcgcg aggagctcgc gtggcgcctc accgaggtcg cggagctcga
    12661 ggagagcgtg tggaaatacc gctggctgga gcacctggag ggcgtcgcat tgaccgacga
    12721 ggtgtacgtg cagccgacga cctcgtcggg cctggtcccg cgcgagtatc cgtacataat
    12781 cctcatcgat ccgcgcgacg cattcggcga cgggcaccat cccaccaccg cgctgtgcgc
    12841 cgatgcgctc tcgacgataa cgaggagcgt tccgatcgaa cgccgcgcgc agatgcgcgt
    12901 cattgacgtg gggaccggca ccggcgtgct cgcggtgctg gcggcgctcc tcggctacgg
    12961 gcgggtggac gccatcgaca tcgatccgga gtcggtcgcg cgggcgcggg aaaattttgc
    13021 gcgaaacgag tgcgctcgca tcggcgccgc ctgcgctcag gtcgcggaac acgcggccga
    13081 cgcgccctac gatatcgtga tcgcgaacct gttgagcggc atcatcaccg acaatatcga
    13141 tcacctccgt cgcctcacca ccccgggggg gacgatcatc gcaagcggcg tgagcgatca
    13201 gtggcatgac gagatgacgg gcctgtttcg cacgcaccga cttgtcgtgc atcgacacga
    13261 tcggaaggac ggatggaacg gatatgtcgt tggcgcctga atgagtgagg cgccgggaga
    13321 ccgccatgga ccgcatcgag gcgcaactca tcatcgacat cctgaagacc ggcagccggt
    13381 attaccggcc cctggaccga gagttttcca gcgaggacga gggcttcttt atttggtacc
    13441 gacccgagtc ggacgacttc gtggtgcgca gatacgaaac ggacgactgg gcggtgcacc
    13501 ggcccggatg tgacgaggag atggtggtat ccgagtcggt gttgatgcga ctgttcaccg
    13561 aatacgggcg agacaatgcc ctgtggccgt tatacgacac aaaccgctac cgcttcgatt
    13621 tcgacacgcc gcagccccgt aaaacgatcg ggcaggccat ccgcgagagc atgcatgcgt
    13681 ccgacctggg cagcctgcgg tgcagccact gcgcaagccg cgacgtggag ccgacggagt
    13741 actcttcgga cgtgaacccg gcccgggcga ggcggtatgt ccatctggcg gtggagtgca
    13801 cacgctgcgg gaggacgtcg gtgtacgaat gggatgatta agcgccgggc gctttttttt
    13861 actccgcaag catcgccagg atgagcccga agaaaccgcc gccgccgtcc gtatagcacc
    13921 atccgatatg attgaaacag ccgccgcaca gggcgacgct ccaggcgtag ccggcgaacc
    13981 aggtgtattc ctccgtcggc gcgccggcgg tcacgcagcc cggcgcatgt gaaaaacacc
    14041 cgatctcgta ggagatgccc gcgggattgc tgaagacatg gcggtgggcg ccctgcatgg
    14101 acatcgccgc gctccggtgc gtgatgatgg cgccgcatat gcggcatcgg agcggacgct
    14161 tgtcggcgtc atcctcgagg ctcgacgcat cgtcggggag ctgcccgccg tcccgggcgc
    14221 cggtggtccg gaaggcgtag atggcgggat catgcggaat ggtcgtgtgc gtctgcgtgt
    14281 tcaatccagt acacctgctt gtggccgcgc gattcgtaga gcgacatttt tcgaaccgcc
    14341 ggttcgtact cgtcgaaagt atcaacgagg caggcccgcc cgtgctcgtc ctggcgcatc
    14401 agccgccacg gacctcgatc gtatgcatcg ctcatggcgc tctccgcggg cgacggcccg
    14461 cgtcatgagt gtgctgaaga gtgcggcccc cgtcaaattt tttcgcatgc aggggaactt
    14521 tttttatcgg ttgacgatcg tatctgaggg tggtatatgt tacgcctctt tgattaccta
    14581 accgtgtggg gaactaccat gaacgcgaaa cgattcattt gcgcaatcct gagcatgtcg
    14641 ctcgccgccg cgatgctgta ttgcggaagt cccatcccgc gtgctagtga cgatacatcc
    14701 gccaccgacg ccagacgcga taccagcgcg cggaaaaccg atcccgcccg cccgtccgat
    14761 cttgccctgg acgaaaagga atacaagagc gctgcgaagc gtggcgacaa gaaatcgaat
    14821 gggagaagcc gttccgatgc ggacgccccc tcgggtgaat cgaaaccgtc caccgcgaaa
    14881 cgcgaacggc gtcccggggc ctcggggctc aaggcggggt tcgtcgatga caacgagcag
    14941 ttcaatcgat acctggagtt cctgaaaaaa ttcgggaaca cggcgcggaa cattccgttc
    15001 gatatcggcg agcgcatcat catccgcgtg cgtgacgcga acggaaagcc ggcggccaat
    15061 gcgctggtca cggtgaagag cgggtaccgg acgctcctca ccgcgcgcac ctatgccgac
    15121 ggcacggtgc tgttttttcc cgtcgaatac ggttcgcagt cgagctatcg ggcgacggcc
    15181 gagttccagc agcagtcggc cgacgcgacc ttcgaacgga cggggaagcg cgaggtggtc
    15241 attcaggtga aaacgccccg ggcggtgatg cagcgcgtcc cgctggacat cctcttcgtg
    15301 ttcgatacca ccggcagcat gggcgaggag atcaaccgcc tcaagaaaac ccttgagatc
    15361 atttacctga acctgacggc gctctcgacc aagccgctgg ttcgctttgg catggtgctc
    15421 taccgcgatc ggggcgacga ttacgtgacc aaggtagtcc ccttcaccga ggacatcgac
    15481 gcgttccaga aggagctcgc ggaggtctcg gcctcgggcg gcggcgacta tcccgaggac
    15541 ctgcaggagg cgctccacga cgccatcaag aaaatgaagt ggaaccggga cgggatccgg
    15601 ctcggataca tcatcaccga cgcgccggcg cacctcgatt acggacagag ctacacctat
    15661 acgcatgcgg tgaaggacgc gcgggcgaac gcgatcaagt tcttctccat cgggaccggc
    15721 gggctcacca tcgacggcga gtatcagctc cggcagatcg cgcagtacac ctacgggcgc
    15781 tacatcttcc tcacctacgg cgagaagggt gatagcgcgg gcggcgcgcc cggcagcgtg
    15841 agccatcacg tgggcgccaa tttcgagacc gacaagctcg agagcatcat catgcgcttt
    15901 gcgcgcaagg agctttcgta tctcacggac aagcccctcg acgacggcga gggctacttc
    15961 caggcgaacc ggatcggcac ggaggacaag aaggacaccg tgaaaaagct cttcacgatg
    16021 gcgatcggcc agctcgtcga tttttcaagc atcgcgatcg cccccaaaac ccgcgcggcg
    16081 gtcatgccca tcggcttcga cgcgaaaaac gccccgctca agctcaatgc ggagtatttc
    16141 accgagcacc tcattctctc gctgggatcg agcaagaagt tcaccatggt cgagcgcaag
    16201 gacctgcagc gcatcatgcg cgagctcgag ctgcagatgg cggggctggt cgaccagggc
    16261 aatgccgcaa aggtgggcaa gatgatgggc gccgaggtgc tcatctacgg ccagctctat
    16321 cggaagaagg accactacga gctttttatc aagctggtgc gcgtggagac cgccgaggtg
    16381 ctgtcggtca cgcgggcgcg gatcgacacc tcactggggc tgtaggcaca gctgcatcac
    16441 cgcgtacatg tattcgccgg tgccgtggaa gaagatcgtg ttgtggtcgg cgccgggaat
    16501 catgagcagc cgcttgtcct tcgccgggca ggcctcgtag agcgccttcg cgtcgctgta
    16561 cgggatgatc tggtcgtatt gcgcatgaat gatgagcgtc ggcttgtcga accgctgcat
    16621 cttgtcgatg ttgcgcatgc cggcctcctc cgtgagcccc agcgccgcgg tgtcgattcc
    16681 catgaggtcc aggagcggca gcgcataggc gaagccgctg tcgatgatga tcccggcgat
    16741 atcgtcgcgg caggcgtccg cgagctcgag cgccgaggcg ctgcccagcg agcgtcccat
    16801 cacgaaccgc tttcccgtga acccgttctc cgaacagtag cgcgaaaaaa aatcgaagca
    16861 gtaatggcaa tcgcgcagca tgcccgtgac cgtcgggcgg ccgctcgacc ggccgtagcc
    16921 gcggtagtcc acggggagaa agttgagtcc cgcctccagg tagagctggg cgatgtcgtc
    16981 gtaatcggcc gcaacctcgc cgttgccgtg aaagaacagg atggagggcg ccgcgcggtc
    17041 cttcacgtaa aggcgcccgc ccacgcgctc gccgttatcg acggggatca cgaggtcgat
    17101 cgcgcccggc acctgcgacg cggaatcgtc ctggcgcggc cagaagaggt tcgagagcac
    17161 ctcgggccgg tcgaaggggg ccaggttgat gcgcgatacg tccttcatgc aggcaggtcc
    17221 tcgaagatcg tcgcgggcat caccgagaac cccggaagga tgacggacgc ggcgggctcg
    17281 tccccgcagt attgtttcac caggcggtag cgcccgttct cgaggatgtg cacgtgcatg
    17341 gtcctgagct cgggatcgac gatccagtgt tccctgaccc caaagcgctc gtagatcgcc
    17401 atcttttcga tggcgtccat ggaggaattc gtcggcgata aaatttcgat aacgaggtcc
    17461 ggggcgccct gtatgtacca tttgtcgcca agcatgctct tgcggtcttt cgcgatgaag
    17521 acaatatcag gttggagtat gttcgtatcg tcgaaatata cgtcaacggg tgcgccgaat
    17581 atctcaccca gcccctcatg ttcaacatgc agtataagag cgtgttcgag ccttcttgat
    17641 attcgttggt gcttccaatt tggcgctggt accattacga gatcgcctcg aatcaattcg
    17701 aatggagcgc tttcgggaag agtccggtag gtttcgattg tatatttcat tctgacgctt
    17761 atagtatata cggatgattg gaggatctat gtcactcgcg atgatagaat acgcgcggca
    17821 ggcaggaaaa gcaagaaaaa tactgcacga cgggcgccgt gtaccagcgc ccgtcgtttg
    17881 gggatggtcg atacggggtt ctagccgatg gcctctttcc cgcgttcgcc ggtgcggatg
    17941 cgaacgcatt ccttcaggtc aaagacgaag atcttgccgt cgccaatctc gccctcccgg
    18001 gcgccgcgcg taatggcggc gatcgtcggc tcgacgaaat cctcgttcac ggcgatctcg
    18061 agcttcacct tgcggagcag ggattcgacc tccttgaccc cgcgatagac ctcggtgatg
    18121 ccgcgctggc ggccgcagcc gaccacgtcg acgacggtgc gtaggtacac cttctccttg
    18181 tcgagctcct tcatgacgtc ctcgagccgg tgcggtttga tgattgcgat gatatacttc
    18241 atggatatcc tcctgtggcc tgtccgttat tcgatgaccg tgtaaccggt ctcgcggtgc
    18301 tgcgtgaggt cgaggccgat gtactcgtca cgcgtgtcga cccgcatgcc gatcaccaga
    18361 tcgacgaact tgtagagcac cgccgtgcca atggcggaga aggcgatcgc ggcgatcgtg
    18421 gcgatgaact gcacccagag ctgtcccggg ttgccgtaga gcaggccgtc cgcgccggcg
    18481 gggttgaccg ccttggtggc gaacacgccg gtcgcgagcg cgccgacgat gccgcccatg
    18541 ccgtgcacgc cgaacacgtc gagcgaatcg tcatagccga aaaacggctt caccttggtc
    18601 acgaaaataa agcagaccac gctcgagatg gcgccgattg cgagcgccga gaccggcgtc
    18661 acgaagccgg cggccggggt tacggcgacc agccccgcga ccagcccggt cgcgacgccc
    18721 agcatggtgg ggaccttgtt gtagcaccag tccagcagca tccaggagac gccggccgcc
    18781 gccgccgctg tattcgtgtt gatgaaggcc gtcaccgcga gggtgcccgc gcttcccgcg
    18841 ctcccggcgt tgaagccgaa ccagccgaac cagaggaatg ccgcgcccag cacgcacagg
    18901 gggagattgt gcggcgcgcg tgaatgaccg accccctcgc gcttgcccag catgagcgca
    18961 aaaacgaggg ccgcgatccc ggcattgata tggatgaccg tcccgccggc gaaatcaagc
    19021 gcgccgatct tcgcaagcca gccgtccttc gcccagaccc agtgtgcgag cggatcatag
    19081 atgagcgtgg cccacaggat gacgaacacc aggaaggccg agaacttgat gcgctcaacg
    19141 aaggcgccga tgatgagcgc cggcgtgatg accgcgaaca tcatctgaaa ggccatgaag
    19201 agctgatggg gaatggtgga catgtaggtg tcgtgcggtt gcaggcccac gccgcgaaga
    19261 ccgatccagt cgagcgcgcc gatgacgccg ccgacgtccg gtccgaacgc gaggctgtag
    19321 ccgaagagca cccactgcac cgagatcacg cagaggatca ccatgcactg catgagcacg
    19381 gagaggatgt tttttttcct gacgagaccg ccgtagaaaa aggccagggt tggcgtcatg
    19441 atcatgacca tcgcggtcga gatgagcatc caaacgatat cggcgttgtt gagttgtggc
    19501 attgagttcc tccttgccaa tatgattcat gccgggcgta cagcgaatgg ccggcactgt
    19561 gttgatataa aaaaaatagc gcacctggtc gcgctgtcaa gagtcgctgc cggcatcggt
    19621 ttgatttccg gggatattta acatcgccga aacagaacac gggcggtgtg cgcgggaaac
    19681 gcgcgatgtg cgcgcggttt gtttacattt gcgtaacaaa gataatgtta ttgaaatttt
    19741 ttcgcggcgc cgtttctatg cacggaagca tatcatgacg atccggagag gtgccatgcc
    19801 gttttcgatg agacgagtgt tcgcccgctg ggcgctgctg atcgccgtta gcgcagccgc
    19861 cgcgtgcagt tcgacacagc tcgagtatcc gctgtggaat ccctatgcgc gcgatgcgat
    19921 cgagcgcgtc atcgttgaaa acggaaaacg cgcccccaac tatacgggcg atgcgcgacc
    19981 gtacatcgtg ctcgattggg acaacacctc aatcatgaac gattgcgccg ggaacctgtt
    20041 cgtttaccag atcgaccgac tggcctacaa gctcaccccc gcggaattct cgcgggtgat
    20101 ccggacgggc atccccaatc tctaccggac ctttcccgcc aagcaccgaa acaaggccgg
    20161 ggccgccgtt acgccgtttc aagtgttcgc tgatgtcgac gcgcactacc agaagctcgc
    20221 gcagctcatg aagaccatgg acgccgccga gataaaactc acggacgtgt tccaggactt
    20281 caaggcgaag atgctctttc tctactacgc gctcgatgac gccttcggcg ccgatgtgtc
    20341 gtataagtgg gccatgtacc tgttcgtgaa ctataccccg gacgagctcc ggcaattcgc
    20401 gaccgagagc catatccaca atctcgacct gccgcttcgc gcggtgacct tcgcgagccc
    20461 cccggagatg aaaacgcgcg ccggggccgt ctccgccgtc catcggcagg gggttcgcat
    20521 acagctcggg atcctggacc tggttcgcgc gtgcgagcgc aacggggtcg acgtgtttat
    20581 atgcacggcg tcgctcgacg agatcgtgat tgcattcgcg agcaatccgc tgttcgaata
    20641 taatattccg cgcgagaacg tgatcgggat gcggctgcaa acccgggacg ggcggtatat
    20701 ggccgcgtat caacagaatt acgcctttaa ttatcgcaag ggaaagcgcg acaatgtgag
    20761 cgagctcatc gcgaaatcgc gcggcgggcg cggtccggtc atcgtggggg gcgattcaga
    20821 cggcgactgg gagatgctct ccggcttcgg cgacacgaaa accgggctga tcctcaataa
    20881 atatcgcgag gggaacggga tcgccgtcgg cgccttcaac gccgcgcgcg cgcagcgcat
    20941 gaaggagccc gcgccccgct atgtcctgca gggccgcaac gagaacaccg gcgagtgggc
    21001 gccgcgcatg gaggtcctcc tgctgggtga tgaagacgac gaggataact ataagcttat
    21061 tcagctcccc aagaagtgac cgcccggcgc taaatgcatc acgatgattg atcttgatca
    21121 catgcccgcg gcggtcaaat gggccgcgct ggtgttggcg acgggatttg tcgcgcagtt
    21181 cggaaagatg ctggccgagt atgtggtgaa gcgataccgg gagcgtcacg cctcgtcgaa
    21241 tacgccccag gcggccagac cctccggcga aaccaccggc ggcgcggagc gacgcgcggc
    21301 atcatcaccc tttgagggcg ccgcagtgcc gcccgttgat tcgaaaatcg aaaaaaagat
    21361 gcgaaaggcg cggtataaag cgaataagaa gtcccgggat cggtagcccc gggacgcgcg
    21421 cgattacttc gattccttga ccgtcgtgag tttctgcttg aggtcggttt cgagcttgat
    21481 gagctctttc tcggcctgcg cgcgcttgac gcgcccctcc gcctggatct tcaacgtctc
    21541 ctcgatggtc gtgatgaggt cgttgttcac ctttttgagc gtctcgatgt ccacgatgcc
    21601 gcgctccgtc tccttggcga tgtcgatgga gctctgcttg aggagctcgg cgttcttcga
    21661 caggaggtcg ttggtggtct tggtgatctc cttttgcagc ttgagcgcct tgttctgccg
    21721 gaagagcgtg atggcgatga cgatctggtt tttccagagc gggatcgtgt tgagaatgga
    21781 agtttggatc ttctcgacga gggtctggtt gctgccctgg atcatgcgca cctgcggcgc
    21841 ggtttggatg gcgatcatcc gggagagctt catgtcgtgg acctttttct cgaagcggtt
    21901 gaccgccatc gcgaggtcct ggaatttctg cgcgtcggcc gggtcattcg aggcgtcggc
    21961 cttggccttc atctccggga tcgttttctc ctggagctcc ttgagtttga tctgtcccgc
    22021 ggcgatgtac aggtccaggt ttttcagata ttcctggttc ttctcgtaca tgttgtccag
    22081 gagcgcgatg tccttgagga gctgcatccg cgccttgtcg agctcgtcgg tgatcttctc
    22141 gatctggagg ctggtcttct cgtagcggat gatgaagcgc ttcacggaat cgaccagctg
    22201 gccgatcacc gggatcttcg agacgaagct cccgcccgtg gaaaggctgt ccacgtcgag
    22261 atccttgatg tttaccacca ggttggtgag gatgtcgccc acgaagccgg tgtccttcgt
    22321 gcggacctcg ttgaggatgg tgtcggagaa cgacgagatc tcccgttgcg cgccgacgcc
    22381 ataggtgacg accgcctggg aatcgtcgat gtcgatctgc ttggcgatct cctgcacctt
    22441 cttcatgtct tcgggcgaga gcttcgatac gtcgacgact tcctcgttct tttgcacgag
    22501 ccgttcagtg tcacccattg tgtcctccgg tatgtttttc gccccagcgg gcgtagtggt
    22561 tttcgatctt tgcgcgcgcc gccgctggcg ggatcatccc tccgtcgtgc gggcggcgct
    22621 tgctacgatt gtttttcctc ggtcgatggc gcactccccc cgagtccctc catcgtaatc
    22681 atgtttttga gcaccttgat ttcggtttcc aggtccagca cgtcgtcgcg gagcagcttg
    22741 tcgtattgcg cctcaaaggc gcgatgaatg ctgtcgagga tcccctcggc cttctccagt
    22801 gacgccttca cctccgcgga ctgtaccggc tggctcgaca gctccacata gcgtttgagg
    22861 atgttgatga ccgcatcgaa gtagtagctc aggaactgct tagccgcctt gatgtccttg
    22921 gggtccttct tgaaattgtc gtagatgcgc tccacgacgg cgcaaatgtc gtacaccttg
    22981 cgcttgacct cggcgtttcg gatgcgcatg ccgtaggagc gcatttcatt gagtttgcgg
    23041 ctcccctcgg cgagcgttgc gcgaaggtcc gattgcgata cgccgccgga cagaatttct
    23101 acaccctccg ggcgcgcgcg ccgcctgaag atgatgagcc ccgcgatgta gccgccgagc
    23161 gcggcaagga gcgacacaaa tatcccaatg ttgagaaaaa cgtgcaggcc ggcgaagacc
    23221 ccgccggcaa tcgcaagtga aataagctgt cgcggccagc cgctcgattt ctgtttcggt
    23281 gattgtgtca tgggataccg tgcgctttga tttgcatgac agcatacata gccgatacga
    23341 ggaatgcatg tcaagagtta tatcattttc acgacaaagc gcgcgatcgc cggcgcggac
    23401 gtgagacccg gcgattcgat gctgagcagg tgtatcacgc tgtccggctg tttcatgatg
    23461 atgaaatcct cgcagcgggt cccaccgcga aaggcgcgcg gccggttccc ggaaaaaccc
    23521 tcgcgcagcg agggacgaat cgcctcgtaa ttcggaatca tgtcgcgcag gctgtcgtcg
    23581 aagaccgcaa atggcgtttc aatgtcgtaa ttgtccttgc cccgcgaggg ggtctccgac
    23641 ggtccgatga tgacatcggg tccgcacgtc ggggtgaggt gaacgccgag cgttccgtgg
    23701 ccgtggttcg ggggcggata caccgggacc tcgaggggga gcgccgtggt gcagtagtag
    23761 tcccccttga gcgggaccac ggtgaagtcg tcgagtcccg tcatgcgcgc gacctcgtcg
    23821 gcgtacaggc cggcggcgtt gatgacgatg tcggcatcga ccgagccgcg cggcgtctcg
    23881 acgcgaacac ctccgtcgat cgccagcgcc gggcactcgt acaggagatg cacgtcgcgc
    23941 tgcataagaa gcgttttaag cgtggtgagg taatcgccgg tgtcgatgat gcaggtcgat
    24001 gccgagtgca aagcgccaac gcagcgcaca caggggacgc gcttctggac cgtatcctgg
    24061 ggaaccaggc tgatcccttc ggcgccgttc gcgatcgcct tgtcgcgcag cgcctcgatc
    24121 tcggaaatct cgctgcggtc ccgtgcgatg atgaacttcc cggatggccg gtattgcacg
    24181 cccgtgcgat cgaagaactc ccgcgagaga cggttcccct cgacgcacag gcgcgccttg
    24241 agggagtcgg ccgggtagta gatgcccgca tgcagcactc cgctgtttcg cgtggatgta
    24301 tccatgccca gtcgcgagtg gcgctccagc aggaagacct cgtagcggcg ttcggcgagc
    24361 tcgagcgcga tgtggcagcc gacgactccg ccgccgatga caacggcttg tttcatgagg
    24421 tggcgtacct gtgggtgtgg gtcataatcg ggtttgtgtt gttttctggc atctgtatgg
    24481 gtttccccaa tagcaatgcc agcatattac gtcgtcaatc acttcttgtc gcagcacata
    24541 gtggcgaaag ggagcatagt tatgcattcc gtttatatgc tcggcgacaa aatcatccaa
    24601 atattcttca accgcaatcg caacaagata cgaaacggag cgcttgcaca ccttccgcaa
    24661 atcaacgaaa aactcattct cgtccttctt gaacgagatg gaaaaacacc gccacactcc
    24721 ccgaggatcg tccggctggt acgtgaccgt cgaaaaccca cgacgaaagc gcgcgttatg
    24781 tttcatgatt cgcatcagga gcgcaacgat aatatcacgg cgcggcatat tgagcgcacc
    24841 ggccacggcg gagacgcgcc caagcgtatc gacatgaata ttgatagtgg ttttaatcat
    24901 gacacgcacc cggaatcgaa aatgaccgta tggagtatat acgtgtacgg ggcgggaacg
    24961 atgaaccgga tttagcgcgc ggatgcggcg ccgacggtaa tttttatccg aaccgaccgc
    25021 atggtgaaac tcgtgaagcg gtccggcttg agcgccatca gatcgaaatc cataacggtt
    25081 ccatcgggtg ttccggggcg gatggcaacc tggagggcgt ttgtggtttg gatccgccca
    25141 acgccccggc acacattgca ctcgcggtcc cgaccaaggc acagcgggca ctgcgatcgc
    25201 gaggaaagct ccaccagggc gattgcgccc cgcgctgctt cgcgggatgt cacgagtatc
    25261 tccacatcct ggccgaggga gtggatgtag tcgcgccgtt tcatgcgcgg attgaggagg
    25321 cgattgcgga gcaggtccgc aagcgaaaac gaatacttta cccgcgagcg tgggataatc
    25381 ggatacgacg gtgcgccatc gtcgccatgg ggaaagagcg cgcggtcgta gcgggcgcgc
    25441 gattcgcggt cgctcaggag cttgtaggcc gaaattatca gcgcgatctt cttgcggtcg
    25501 gtatcgctgt tcatgccggt gcaatcggtg tgatagcgct tgatcagcgc gcagaacgcg
    25561 gtgcgcactt catcggcgcg tgcggtgtac ggcaggttga atatgtcgta atagttgatt
    25621 accgcgccgc tgtcgtcata ccaggatgcg ttcatggccc gtactccatg ccgagcgcgc
    25681 aatgaagggc ggcgatcacc gcgtcggttg caatgccgtt cgatgatcgc tccccgtccg
    25741 gcggcgccag aaccgttacc gagccgccga tcggtttcca cgccgcgggg tccgtgggac
    25801 cgaacagcac gaccgacggc acaccgagca gaccggcgac gtgcgcgatg ccggaatcgt
    25861 tgcccaggaa ggtgcgcgcc ccgcgcagca aagataccac ctcggcgagc ggacaatcaa
    25921 caaaaaaaga gatgcggggg tgatggcgca tgcgctcgaa ggcggcgtgc agcccgttct
    25981 ccgcatagcc catgaccagc gcgatcggtt cgcgcaggtt cgagcagcag taatcgatta
    26041 ccgcgcaaaa gcgctccggg ggccagttct tcgtcgcgct tccgcttccg ggatggatga
    26101 tgacgccccc gccgcgctgc gccggaggcg gaaaaccgcg ggtgaattcc cggcagtagt
    26161 cgtcctcgga gagggtctgc cggaggccgc agatttcgat gcaataccgc cattgcggca
    26221 cgcgcggatc ggcctgccgt gatgatgcaa tgacgaaatc gcggataccg gctgcggtca
    26281 gacgatgcgc gatttcgccg tcgccatcat caatcaaaaa gaatagccga tcgtaggagc
    26341 caaggatgtc caccgcctcg cggtccgcgg cgcataacag gcgcgtcgat aggcgatcgt
    26401 cgatcgagcg cgcgttcgcg atgcggccgg agatgcgaaa gagctcctgc gccgcccggt
    26461 tgccgatcat gtcgaactgc gcacccggaa agcgcactcc cagctccacg agcgcgggaa
    26521 acatgagcag ggtgtcgccg atcccgccgg gttttacgat gagtattttc attgcaaata
    26581 tgccgcgctc ggccataccg gccatcgacg caaatacaat cagacatgtc ccatgaacgc
    26641 aacataattc tctgtcaacc cgacgcgacg aagagctgcg ccgcgtgctg cggcgtgttc
    26701 aatcttcggg acatcacgga ggacgccctc gcgagatact gcggcgacta cgaggttcgc
    26761 gcccggcgcg cgtatcggct tgcgccggga tgccatctcg agggggccgc cgacctcgag
    26821 aagcgcgata ttggcgcgca tgtctgtccg tatgcgggct tcgcgcatcc cggcaggccg
    26881 gggtgcatgc tgcatccggc cgttcgcggc cgagaagagc gggaccggtc gcttttcggc
    26941 gcgaagattt gcgccgagtt cctgtgcccg gcgcatgcca tcctcaccga tgcggagaag
    27001 aagctgctcg ttggggcgct cacccactgg tatccgtaca ccgtcgccat cgtcgatccg
    27061 gaatcgttcc ggtggatcat cgagcgggtg gcggaggggt atgcgtgctc gccggcagaa
    27121 attatgtcgc atcaagccgc ccgcgggtcc atcacggccg cgctgcgggc gcacgcggat
    27181 tatctcaacg ggatcgacgg acccatcttc cagtactccg tcgcggagta tgcgctgggc
    27241 cgcagtcggt tctctctcgc gggaggtgct gatgcgctgg cgcgtcaccg cgcgcgggtg
    27301 ctggacagca tcgccatcaa ataatattgt tgcgatattt tgcgcgatcc ggcatgcttc
    27361 tgcgtacaca atcgcccgct ggaggggacc tatggagtgt ttcggcatcg ttggtgcggg
    27421 gatgatgggc accgacatcg cacacctcgc gttgagctcg gggttccgcg tcgtcgtgtg
    27481 cgactgcgac gccgcgcagc ttgatcgggc gcgcgagacc ctcggcgcgc gcttcaggcg
    27541 ctatgtcgcc gagggccggt tccgccaggt ggagatggac gggctcctgt cgaggttgat
    27601 caccgagcgg tccatcggcg cgctcgccga gtgcgacatg gtcctcgagt gcgtcaccga
    27661 ggaccggtcc gtcaagatgg aggtctttcg cgccctcgac ggcgccgtga agggcggcgc
    27721 gattctggcg accaacacct cgtccatctc catcacccgc atcgcctccg tcacgaagcg
    27781 cccggaatcg gtcatcggca tgcatttcat gtatccggcg cgggtgatga agctcgtcga
    27841 gctcgtctgc gggctcgcca ccacccgcga gaccttcgag agcgccaagg ccatcgttcg
    27901 tcagttcggc aagcagttcg ttgaatcgca ggattcgccg gggtttttgc tcaaccgtat
    27961 cttcgcgccc atgataaacg aggcggtgtt cgcgctctac gagggggccg ggacggccga
    28021 gagcatcgac aaggcgatga agctcgggac caatgttccg ctggggccgc tcgagctcgc
    28081 cgacatgatg gggctggatg tcgtgctggc ggtgatcgag gtcatgttcg atgagttcgg
    28141 cgatccgaag tatcgcccgt gcccgctatt gaagaaatac gtcgcggcgg gatacctcgg
    28201 aaagaaatcg ggccgggggt tttacaccta ctgattctgc agtgagccca ggaaggcgcg
    28261 gccgtggtcc gtgagcctgg cccgcatcgc cgcggtgcgc gaaccggcgc agtaatccat
    28321 gatgccgaaa tgccgcagcc aggtgaaaag cgaaaagtcg ataaacgaca ccagcatgag
    28381 attgtcccgt cggcccattc cggtcatttc gatgatctcg ccggccgttt cggccaggtg
    28441 aaacgcgccc gagctcttca tgacgatgtc ggccgcgatc ccgcggttta actgcaacat
    28501 gccggcggcc tcaggcatgg acgggaagag gcgctcccat tttaccaggt tccagaacga
    28561 caggaagagc gaccggtacc agtccgcggc gttctcctgc ggcgcgcttt tcttcaggat
    28621 gagggcggtc tgtttgagat agatgtgcgc gagctcgggg tcgcagtcgc agtcgtcgaa
    28681 cagcgtctga tcgacaagtc gatcgagcgc ggcgtcgaac aggatgctgc gatagccggt
    28741 ttcgcatgcg tagcggccgg gatcatcggc gaccagctcg gtaatgatcc gcgcggcgcg
    28801 cagggcgaag gtttcctgtg cgagcgagac ggtcacggga agggttttcg cagccgcagc
    28861 ggcgcgggta cgcgttgtcg aggtgcgtgc gtgctttcga ggggccggca tcgcgagcca
    28921 gatggcggtg tagcaccgtt ggacgacctg cccgaggatc cgttcgcggt cgctccgcgt
    28981 cgccgtggta aaacggctct cgatctcccg ccggaccatc tctcttgaaa aatcatcatt
    29041 gatcaccttc cgcgcgagaa aaccgatctc gcgggacagc tgctgtccgg cggcacctga
    29101 cgagttcttc attgtatatt cgagacccct ttttcaaaaa acaacatgac cagcgtgccc
    29161 cctcgggccg gccatgtgta tgcgtcgtac aatatatatt atccgctcga gaggtaaaac
    29221 atgggacata atgttatacc gtcaaattaa tttcaacaac tattctgccg taaaaccggg
    29281 ccgccgttat tttttgcgaa attctcttga taaaaaaggc ccgattgcaa gtatgttctg
    29341 atccggacac cgctgcggaa cataccaata cgccatgagc atcatcgatc gataccggaa
    29401 tcatcaggac gtcaaggaaa ttcgtaaaaa cgccgaacgg atcctgcatc gcctgtccgt
    29461 cgagattggc gagcgcacgc tccggcgcta cgatcgtctc gaggaaacac gggccttcat
    29521 caccgaccgt ctctccgcga ccgggcatgc gtacgccgag gaatacacgg tcgaggggaa
    29581 gacggtcgcc aacatcgtcg cggagatccc cgggaccgag cgtccgaacg agataatcgt
    29641 catcggcgcg cactacgata ctgtcgaggg gacccccggc gccgacgaca atggctcggc
    29701 cgtgacgggg ttgctcgaga tgtttcggct gctcagcgcg cgcgcctatc gccgcaccct
    29761 ccgcttcgtc gcctttaccc tcgaggagcc gccgtatttt ctcaccaagg atatggggag
    29821 catgcgctac gccaaggggt gccgcgagcg caaggagctc attcggttca tggtgtgcct
    29881 cgagatgatc gggtatgccg ggcgctccat cgagcagcaa tatcccatcc gcgagatgaa
    29941 ggagcgcttc ccgcgcatcg gggactttct cacggtggtg tccctcccgt cctcgtcccc
    30001 gtacacctac ctctggaagc gggtgtataa tcagcacgcg aagaaggccg ttcacgagct
    30061 catcgggccg gcctccatac ccggcatcga tttttccgac cactgttcgt tctgtagaaa
    30121 tggctttccc gcgatcatgc tcaccgatac ggcgttttac cgcaacaagg cttatcatac
    30181 cgaacacgac acctacgaca ccatcaattt cgattttctg gccgatgtga tccgcaacag
    30241 cgcccggacg ctggccgaga tcgccgacat ggacgaactg cccgtcgccg agtgcggctg
    30301 ctagcgggga tttgcggacc cgcgcgatta atgaattgac aggatgctct tggcgtggcg
    30361 tgatcggggg cgcggcggcc gctttagcgg gcgcctatct gaaatacacg gagcggttca
    30421 cgaatggccg agaaaagcga tcgccaggcg cggctggcgg aagacaatat cgtcaacagc
    30481 atcatcggcg aggggtccga attcaagggt gagttcaagg tcaacgggct cctgcgcatc
    30541 gacggcaagt tcaagggaac catcgagacc gagggcaagg tgctcatcgg caagagcggc
    30601 gaggcggtca ccgacatcaa ggcgagcatc gtgatcatcg gcgggctggt cgaggggaac
    30661 atcttcgccg gcgagcgcgt ggtgatgctc tccagcggcc aggtgcgggg aaacgtgatt
    30721 acaccgagcc tcgtcatgga ggacggcgtc gtcttcgagg ggaactgcgt catcaatagc
    30781 tgattgcgcc ggagcgccgt tatgatcgag atcatcgcca accatcccaa caaggacgag
    30841 cgcaccaagg tcaaggggaa gaagcgttcc ggcgcgcccg ccgggaccgc gcgcgcgttt
    30901 cgcgaaccgc tgcagcaggc ggtcgccgcg gaagtcgagg gcaccatcga tgatctcatg
    30961 ggcaatctgg ccgagcagga gaaacgcttc ctggaccagc agaacctgta cgagatgggg
    31021 cgctacaagg cgctggtcca gaagatcctc aagatggtcc tggacgaggg ctacacgacc
    31081 gccgtgctca agcgccacag ccggaacaag gccgacttca tcatcgtcga tcgcatcaat
    31141 gcgcgccttg ccgacatttc gctgcacatc acgcgcggca cgagcggttt caatctcctg
    31201 aaaaccatgg aggagatccg ggggctcgtg ctcgacctgc tctcgtgacg ccggcccgac
    31261 tcgccgcgca tgggtgcgtc gggaccgccg catgccctcg cgacaaatcg cgttatacga
    31321 tagatgttcc gtgctgacct tttccgacat cctcggccac gacgagccgc tccgccgtct
    31381 tgccgggctc cgggaccgga acgccatccc caacaccctg ctcttttccg gcccggaggg
    31441 aatcgggaaa cggctcgttg ccatggcgtt tctcgcatcg ctgttttgca gaggcgatac
    31501 gcggccgtgc ggcgcctgcg ccccctgccg gcagatcgcg gcggacacct ttccggacct
    31561 ggtcatgctt cttccgaacg acaagggggt gatcccgatc ggcaatgccg agaagcgcga
    31621 gccggggagc gtccgctggc ttgtcgatcg gctggcccgg cgctcggtgt cggggcgcgc
    31681 ggcggttctc atcgatggga tcgaccgggc gaccgacgag ggccagaatg cgctcctgaa
    31741 gagcatcgag gagcccggcg agggcgcggt gatcgtgctg gtgacggccg accgctcgcg
    31801 cgtcctgccg acgatccgct cgcgctgttt cgagctcgcc tttcgccccc tcgacgatca
    31861 ggccatcatg cggcatcttt cgcgcggcgg cgttgacgag gacgacgctc gttttatcgc
    31921 gcacctcgcg cagggatcgt tcagcgccgc cggtctcctg atggagggaa cgacctgcga
    31981 ggagtgtctg gcgcttgccg gcgagatttc cgcatttctc attcgcgggg agctcctcac
    32041 gctcgacctg ggcgggctcc agaagcgcct gagcgccgag gcgatcgtcg atacgctcac
    32101 cgccatctac cggctcaata tgcgcgttct cacgggcgcg cgcgacgtcg ctcccgcgaa
    32161 agaggcgcgc ggggcgctct atcccgtttt ttacgagcgc gtttgtattg acaatttatc
    32221 aatggtgacg gcgcttgtta aaatatttct tgccctcagg gcgggtcttc gctataattt
    32281 aaacttgcgg aattccctca aagggagcct gtaccgggcc cgagagggcg ccgcgggcga
    32341 cgacgcctcg cccgatacgc tgccggaacg cgggtgacct atggatgtag ctctcatacg
    32401 gctgcgcaat accagccaga tatcatgcgt gaacaccaac aacctgttca tcaagtccgg
    32461 gacgcactgc gtcatcgaga tggacaatgg cgtggacctg gggcaggtcc tgaagcgtca
    32521 atcgtcggtc aagacaaacg gcgccgaggt gaaggggaag cttctgcgca aggccacacc
    32581 ggatgatttc aagcgcgtcc ccgagctcga agcgctcgag aaccgcgcgt tcgcggtgtg
    32641 ccgcgacaag gcgcgcgagc ggggactggg catgcgcctg gtctcggtga aatgcctgtt
    32701 cgataaaacg aaaatcatct tctacttcgt cgccgagaac cgtatcgatt tccgcgaact
    32761 ggtgcgcgac ctcgcctcgg cgttcaagac gcgcatcgag atgcgccaga ttggcgtgcg
    32821 cgacaaggcg aaactcatgg ggggattcgg cccctgcggc cgggagctgt gctgctcgtg
    32881 cctcaaggag gagttcgagc cggtatccat caagatggcc aaggagcaga acctcaatct
    32941 caactcgctc aagatctccg gcatgtgcgg ccggctcctc tgctgcctct ggcacgagta
    33001 cgatgtgtac cgcgagctca atcgggacct gccctcggtg ggaaacgagg tgcgcaccga
    33061 gaaaaagacc tacaccgtcg attcggtcga cacgctcaag cagcagatcc ggctccgctc
    33121 gcgcgattgc gtgctctgcg tcgccaagcg cgacctcacg cgcgcgggcg atcactatac
    33181 cgtgtccgag gagctgatcc agcagaagat caagcagttc gaggacgatg aaaacgccga
    33241 ataactgaac cggagcgcgc gatgcataac cgcttttacg tgaccacccc catctactat
    33301 gtgaacgccg agccccacat tgggcatctc tatacgaccg tgctcgccga tttcctcacg
    33361 cgattttatc gcctgcgcgg gtacgacgcc ttcttcctca ccgggaccga cgagcacggc
    33421 gacaagatcg cccgcgccgc cgccgagaga gggcgcgagc cgcaggagta cgccgaccgc
    33481 atcagcgccg tgttccgcga gacctggcgc gatatcggga tcggcttcga tgattttatc
    33541 cgcacctccg agccgcggca ccggcgggtg gtgcaggcga tcctgcagcg cgtctacgac
    33601 gccggcgaca tctatttcag cagctacggc ggccactact gcgtgggctg cgagcggttc
    33661 ttcaccgaga aggagatggt ggacggcaag tgtccggatc acgaccggcc gctggagttt
    33721 gttcaggaag agaactactt cttccgcatg agcaaatatc agggttggct ggcggagcac
    33781 atcaacacgc acccggactt catccgcccc gagcgctatc ggaacgaggt cctctccatc
    33841 ctgaacaacg aggtcctcga ggacctgtgc atctcgcgcc cgaagacgcg cctccagtgg
    33901 ggcatcccgt tgcccttcga cgagcgttac gtcacctatg tgtggttcga tgcgctcatc
    33961 aactacatca gcgccctcgg ctttccggac gattcgcgct tcaagaccta ttggccggcc
    34021 gcgcagcatc tcatcgccaa ggacatcgta aagccgcacg gcatcttctg gcccaccatg
    34081 ctcaaggccg ccggcatcga gccctatcgc aacctcaacg tacacggcta ttggaagatg
    34141 gaggacgcga agatgtccaa gagcctgggg aacgtggtgg ggccccgcga cctcatcgcg
    34201 cgcttcggca acgaccagat cagatatttc tttttgcgcg agatgacctt cgggctcgac
    34261 gcgaaattca ccgaagaggc cgtgataaac cggatcaact acgatttggc gaacgacctg
    34321 gggaacctgg tgaagcgcag cttcgcgatg acgcagaagt atttcgcggg ccgggtgccc
    34381 gcccgttcga gcgccgacga cgccgggcgg ggcgagctgg ccgcgctcct gaaggcgtcc
    34441 gcggacgaat atagtgcggc ggtcgagcgc tgccagttca gtgtcgggtt ggagcggctg
    34501 tgggagttcg tgcgcgcgct caaccggtac atcgacgagc ggaagccctg ggcgctggcc
    34561 aaggagcccg gttcgccggc gctcgcctcg gtgatgcgca atctcctgga atcgattcat
    34621 gcgctggccg tcctacttgc gccggtgtgc gtagacggat cagccaagat cctcgccggt
    34681 ctcggtcgcg gggatacggt cgtcgccatc gacacgcttt tttccctgga ctgccttgcg
    34741 gacggccagg caatcacgga ccccggcatc ctgtacccgc gcatcgaacg cgaggagacg
    34801 ccggcggtct ccccggagaa ggagaacaaa ccggtgaata ccgaacaaga cggactgctc
    34861 gacatcggcg attttgcaaa ggtcgatatc cgcgtagcga aggtgctcac ggcggaacgc
    34921 atcgagggat cggacaaact gctcgagctt cgcgtcgaca gcggcctcga cgagcgcacc
    34981 atcatcgccg gcatcgcgca acactacgaa cccgcctatc tcgtcggaaa gaagatcctg
    35041 ctggtggcca atctcaagcc ggcggtcctc ttcaagcgca cctcgaacgg gatgctcctt
    35101 gccgcgaaga aggacaagaa ggacgcgccg gtcctcatcg aggtggacga ccgcatcccc
    35161 gtgggggcgc ggctctcgtg agggcccatg aagatcggca tcatctccga cacgcacaat
    35221 aacatcgaga tcaccctgaa ggcggtcgcg gtgttcaagg agcggagcgt ggagatggtg
    35281 ctgcacggcg gcgatctcac ctcgcccaag atgatggagt ttttcaagag cctctcgtgc
    35341 gattgccggt tcgtgctcgg gaacagcgat atcgattccg acctgctcaa cgatcgcgcc
    35401 caacggctgg gattcggctg cgtcgaggac acctgcgaca tcacggtggg cggcaagcgc
    35461 atcctggtct tccacggcga caatgtcccg ctcttccgga aggcggtcgc atccggcgag
    35521 tacgattaca tcatcaaggg gcacacgcac ttcttcgaaa actacgtgtc caataacacc
    35581 cgcgtcatca accccggatc gctcttcggt gacgacgaac acacggtcgc cgtgctggac
    35641 acggaaaccg atcgcgtgga gaagattcgg atcgaaatgg agtgagtcga tgcctcgcca
    35701 cgagttctcc tctttgtcgt agatcgaaaa catattgact ttttcttcgc aagccgtcat
    35761 ggtaagacca ccacaatgac acggagatgc gcgcgttatt cggtggattt tttctatatg
    35821 tccacttcag cgcaaagtgt aattcctgca gtaccatgaa caaggcgacg aacgcagttt
    35881 catcaatcgg aggtaacgca tgaaaatcat catgacgccg gacgcaccca agccgggcgg
    35941 gcactacgcg cacgcggtca tcgaaaacga catcgtctac gtctcggggc aacttccgct
    36001 cgatccgacg accggcaagc tcaagggaaa ctccatcgaa gagcagacgg agcagtgcct
    36061 tgcaaacgtc gcggccatcc tcaaggccgc gggcagtcgg cccgatctgg tcctgaaggt
    36121 gacggtctat ctgtcgaaaa ctatatggct cggcaaggtc aatgacatct atgctcgctt
    36181 cttcggcgcc cacacgccgg cccgggtcgt ggttccgatc gaagaccttc ccaatgcgat
    36241 gtggatcgag atcgatgcca tcgcggcggt tgcgaaataa cggcatcggc tctttgcacg
    36301 acaggctacg agtaatgggt ccggcctaga gccggacccg tgccgccgca tcgggctcct
    36361 cggccgtcac ggccgctcgt cgatctcgat gcggttccgt ccgttgcgct tggcgcgata
    36421 gagcgcgtca tcggcgaact tgatgaccgt gtccgtgttg gcgattcccg caagctcgga
    36481 cgcaccgacg atgacgatac cgatgctgat ggtgaaccgt atgtccgctc cggtctccga
    36541 catcgtcgag gaggattcaa cgaggctgcg gatcttttcg gcgaacagca tcaccccgtc
    36601 ccggcccgct tcggggatca tcacgacgaa ctcctcgccg ccatagcgcg cggcgatgtc
    36661 gctggcccgg gtgtttcgca tcaacagttc ggagaggttc ttcagcgcga cgtcgccgat
    36721 ctggtggcca tgggtgtcgt tgatggcctt gaagtggtcg atatcgatca tcatgatgca
    36781 gaggttcttc cggtagcgga tcgcgcgatc gcattcctcg cggtatcgtg tgaagaaata
    36841 gcgccgattg tacagccccg tcagctcgtc gtgcgtggcc atctcttcta tcaacacctg
    36901 cgcctccgtg atcctccgga tgagctttct gatcagtaga tatatgatca gaaagatgat
    36961 cgcgaaggtg gccacgccga gaatgataat gacgaccatg tagaggttga tctgtcgcct
    37021 ggtgtcggtg atgtcgaagc tcacgctgat gccgccgcgg atgtccccga tccggtagcc
    37081 ctgttcggcg tggcaggtga gacaggatcg gtcgacgaca aggggcgcca tgtagcggaa
    37141 gaggtatcgg tcggcgctgc ggacgacggt gctcgtctcc ttcgttccgc gttcgaatga
    37201 acgcagcgcc ttttcctcga acggatcggc cttgttcccg ggattgagcg gcatcaggct
    37261 ggtgatgtga aaacgaaaca gcccgtcgcg ttcggcgatc tgcgagatct cgcgggtcat
    37321 gagcgcggga ttcttcttcg tgtacgtctt gccgtttatt ccgtggatgt cgggattttt
    37381 taaatagggg ttcgattcga cgcccttcgt cttttccaca tagaccccgc cgtagcgcgc
    37441 gttccagctg cgggtgatca cgatgttcct gaaatgcgcc cgtgcgttcg acagcatgcc
    37501 ggactcgatg aggtccttgt tcttgaaaaa gatgccgatg aagatcccca cgataaacag
    37561 caggatcacc agcatgatgt tgaggatgaa gggtttccag atatttcgcg agtttttttc
    37621 aaatagttcc gacatagtcg ttcctctgct gcgcgatgat tatattagta tgatatatgc
    37681 acggtcaacg atattattgc agaaacttcg cgagggcata tcgcctgaac gctgtgctca
    37741 tgcatgaaga cggtgagggg aattcagtgc gcatgattca ggagaagaat cagcgtacta
    37801 gagaatgtga cggacgtcgc accgcctcga tgctgatagc gcctcgtccc atgatgggat
    37861 ccctcgtcgt cccccatacg cgcgacacga gagggtcgcc acggcgaccg cgaaggcgag
    37921 aagctcctcc cgcggcaggc cgcgggccag gccgagggcg aaggccgcgt ggaagttgtc
    37981 gccggccccc gtcgtgtcct tgacggtctc ggggggagga gcggcggtga tgatttcctt
    38041 cccggtgaac catgcggcgc cgcggggacc gtcggtcacg atgagctctc cggtgacgtg
    38101 agtgcggaaa tccagcgccc ggtcgaacaa cgtgatttcg gcgccgccat cgctatcggt
    38161 gagaaaaaac tccgccgagg gaatgaagta gtccgcggcc gccatcatgt cctcgagtcc
    38221 ctcgcggcgc cgctcgcaat cgtacacgat gaggccgtgt tgtgccgcgc caagcatggg
    38281 agccagatag gtcgcctgcg gatcgaggag tatcaccggc gcggcggcgg cgtgcgacat
    38341 cagcagatca tcgacgacag gctttgggag cgctgagggc tcgtagaaaa tggtccgcga
    38401 gccgccgccg gcaattacga tggccgctgt cggcgtccgg ccgccgggaa tgattttaac
    38461 cagatcggtg gggacgccga aatcggcgag gcggtcgagg cagaaacgcc cggcgtcgtc
    38521 gtccccgacg gacccgatga aacgcacgcg gccgcccagc cgcgcgacgc aacatgcggc
    38581 ggtgcttccc tgtccacccg cctccacgag gtgctccgcg actgcgatct tgcggtcctc
    38641 ggggggatat gagtcgacga cgtacaggta atcgacgcag cttcgcccga tgaccagaac
    38701 gtcgacgtcc aatgaagaaa tcgccatgat gccctccgct gcatgctggt acgatgctac
    38761 gagagcgcat ggtacattgt caaccgggta ttccggatca accactctgc cgatgctgcg
    38821 ccgtattctt ctttacactc acggaaatgc ggtgcgtgat gggcggcatt ccattacgga
    38881 ccagaatgcg gagaggaaaa atgtcgtcat tgaagagtcg gttgacggcg atcttgaatc
    38941 tcgatacgcg aaaccagccg tattccgcga cgcagatcgc cgggatgatg gctatcgtcg
    39001 tgctgctgtt tccgctgctg ttcaccatgt tcggatttct atcggcgcca ctggcgggcc
    39061 tgattgaagt cgttttccgg ccaagagatc cggacgcggt ccgggcggcg aagatcgcgg
    39121 tgctcattgt caaaaccgcg ctcggattga ccggcgggtt gtggatatgc cgcatcgtgt
    39181 ggccgaaaag attggcgttg ccgagggatc agggcgatga gcatccggtt tttccaaaat
    39241 aaagcaatca ccctgccaat atccatcgcg gtcatggtat gtgcagtgta catggcggcg
    39301 gttccaagga ccggaacgac cgctgaggtc gaatcaacac ccatgtatag caccggcgat
    39361 attctgaacg cgcgcgcgaa gccgtcgatg agcgcgccga tcgtcgtgca gataccgtac
    39421 ggatctatcg tgaatgtcgc gaaaaccgat gtgcgcatga cgtacgaggg catgacgtat
    39481 cactggtatc gcgtcaagga ggccgatgcg tatgtcctgg gcgcatttct ttccgtgaaa
    39541 cgaccatcgg cgaaaaagga gatgaagctc tgcttgtggg gcgagaatga gggatgtccc
    39601 gcgcagagcg cggtggtgcg cctttccgga atgaaggcgt catcgtcgcg ctaccagggg
    39661 tgtcattcgg aagaataccg gagcgagctc tcgggggact acgcgatcag gaaggggtat
    39721 ctggagattg tatggaagga ctatgaaacg atacgcaccg tcattgctcc gggacgggcg
    39781 cgatcatcga ctcgcgttcg caataaaggc tatatccagc ggttccactg gcgagcggac
    39841 tacgcaggct ggatcgacga tgacacgctc gcgctcatgc aaaaacggaa atgccggctc
    39901 gacccgaaga actgttttct cgattgcacg gacccctcca tcaccgacgc agccgagcgg
    39961 gagatggacg ccaatgaaaa tattcgttct ttttactgcg tcaaggaatg caaataggat
    40021 gatatgagtg tcggccgcag tgcgggttga cgtgatggtc acgtgatcaa ctcgacgatc
    40081 cggtcgaagt cgtcgagcga gtagtagctg atctcgatgg tgccgtgcgg tccggcgtgc
    40141 ttgatctcca ccttggtccc caggcgggac accagaagct cctccatctt gcggatctgc
    40201 gggtttttcg atttcggttt cgccggcgac ggtttcttcg tgccggcgct tccggcgaga
    40261 cgcgattcga gcgcgcggac ggaaagcccg ttgtcgatga tctcgcgaaa cagcgttttc
    40321 tgctgggcgc cggaggtccc gagaaggacc ttcgcatggc ccacgctgag ctcgcccgcg
    40381 gagatgctcg cctgtatctc ggaaggaagc gcgagcaggc gcaggagatt cgcgatggtc
    40441 gcgcggtctt tccccacgcg cccggcgata tcgttctgcg tgaggctgaa tcgttcggag
    40501 aggaccctgt acaccttcgc ctcttcgatg ggatcgagat tggcgcgctg gatgttctcc
    40561 acgagggcca gggtgatatt ctcctcctcg gaggcctgga tgacgatggc cctgatcttc
    40621 ggcacgcccg caagcttgga ggcccgcagg cgccgctccc cggccacaac gaagtaatcg
    40681 gaccccgcgc ggcgaacgat gatcggctgc agcatcccga ccgatcggat cgattccgcc
    40741 agacccttga tctcgttctc gtcgaagtgc gtccgcggct ggtcggggtt cggcttgatg
    40801 gcgttcgtgt cgatgtgcac gatgcgatcg ccgtgcgcaa tcacctgctc ctcgaaatcc
    40861 gcgaccggcg tcgtcgattt cgagatgatg gcctccaggc ccttccccag cgccttctta
    40921 gccattggcg agcacctcca acgcgagctt ctcgtagctg tcgctcccgg tcgagtgccg
    40981 atcgtacatg ccgatcggtt tcccgaacga gggcgcttcg gagaggcgga cattccgggg
    41041 aatgatggtg gtgaaaacct tctctttgaa gaattcacgc acgtcgctca ccacctgctg
    41101 ggcgagattg gtgcgcgggt cgtacatggt gagcaccacg ccctcgatcg ccaggcgcgg
    41161 attgagacgc tcctggacca tcgcgatgat gcggagcagc tggctcaacc cctcgagcgc
    41221 atagtattcg cactggagcg ggatgaggac ggagtcggcc gccaccaggc tgttgagcgt
    41281 caggatgccc agcgacggcg ggcagtcgat gaagatgtag tcgtactcgt cgcgtatcgc
    41341 ctgcatcgca ttgcgcaggg tgaattcctt gccctcgagg tccaggagct ctacctgggc
    41401 gcccgagagg tgtatgttcg acgggacgat ggacaggttc ggcaccgagg tgggctggac
    41461 gcactgcgcg atggtcttct cgccggtgag cacctcgtag acggtgctgg tgatctcctc
    41521 gacgcgcacg ccgaggccat aaccggcgtt cgcctgcggg tcgatgtcga cgatgagcac
    41581 gcggcggccc ttctcggcga tgaacgccgc gatgttgacc gtcgtcgtcg tttttccaac
    41641 gccgcccttc tggttggaaa ccgaaatgat cctgcccatg cgccgctccg ggtgtgccgc
    41701 tatcgtcgga ctctgccgcg aaacctcttt gtaaaaaaga gcgttttaat tgcaagaatt
    41761 aaaatcgcaa ggcacgccac ggccgctgcg ccgtagaggg cgtagcgtac atggggacgg
    41821 gcccgtcggc cggacgcggc gaggagcgca tacggcgtcg cgcgcacgcc gcgggacggg
    41881 tagaggtgga cgcgcacggt gcgatcgcca tccgagggaa tatccacaag gaattcgcgc
    41941 tggtccaagg aataggcggc cagcagcgag tcgctcgaaa atacgtcgag cgagagctcg
    42001 tcgcccgcgc aactgacata gagcgtgttc attcccgatt gcagcgggac cagataggcg
    42061 cgcaccgctt caccggcgag cacacggtcg gcgatgcgga cccgcacggt ctggccgttc
    42121 tcgcgcgccc gctcctcaag ggacgccacg agatcgcgat ggtacagccg gtacgcgagg
    42181 aagcggaaga gctcggtgat gcggtcctcg tgatggctcg aaaacgcgat agtccccttc
    42241 ccgcgcgaga tcagcgctgc gacgggtccg gagcgctcgc cgcgcggggt cgagaactct
    42301 ccggtcgcga gcggaacggc gccgctgatc gactccggtg caacccatcc cgaatgaagc
    42361 acggttcggg tgtcgtggcg cagccgggtg aaggccagga ggtccccgtg gtgggtcaat
    42421 tcgaccgttc ccgcgcggcc cacattggga aagccgtcga agaatgagaa catggaaaag
    42481 gcgcactggg cgtattcgta cgaaaggtct gagaaatacg ccgccccgcc gttttcgaca
    42541 tactggcgga tggagccgca tacgcgcgcg cggttcacgg aatagtgatc gtcggtcagg
    42601 gtgacggcgt tgagccggat gccgtgcgag cggacgtcga tcgcgtcttc gattgcgggg
    42661 ccgatcccgc agggaaaaaa caggacccgg taggttcgcg cggggcgctc gaggtccttc
    42721 agtcgtattc gctcgaaggg gatggagagc tgtcgcaatg ccgcgccggc gttttcgaac
    42781 tttccttcga caaggccggc gggctgatcc gcattcgcgc gcgtcgcgtc gcgggccgtc
    42841 tgcgagaaca gcgggagaat catgagggcg ataacggcaa cagtgcgcac ggcgaatcag
    42901 ctcgtgaggt cgcggatgcg ctttatgatc gagcgccgct cgtcgaggag ccggtagagg
    42961 gagctctcct tgcaggcaaa ggcgagctgg taacagaatg cgatggcgac gagcaggaag
    43021 ccgggatttg cgagatcacc gaccacctcg cgcgccacga gatacttcga tgcctgtgcg
    43081 cgcccaggaa aacccttgat cgtgcagtcg aagaagggga actgccggcc cgcctcgtcg
    43141 gtatcgtcca cggcgctccg ggagagaagg ggcgtgcgtg catcgtccgg cccgtcggat
    43201 gatgcgtcgg aggcgcgcca gcgctggtgc gttcgaagca ggcggtggca ctccgacccg
    43261 atctggtaga aaacgctcgc aaggcgcccg aggtagtgct gcaggtgcgc aagggacttc
    43321 gtttcgccgc tcaggatccg ctccacctgg gtgcgcgtga cgaccagggt ggggttgttc
    43381 gacttaaaat aatggatctc gccctggagc tcgagaaaca gcgcgcgctc cttttcgaaa
    43441 taggcggggc cgaagagcgc ctcgtcgcgc gtttcgcccc gggcgtcctg aagcgtggcg
    43501 cgcgcgccgg cgatgatcgg cagaatcaca ccggcgaaaa aggcgaggca tccctccatg
    43561 aatccgaaga aattctcctc gtgctgcgtg cccggatcgc ccttcttttt gtcgatatgc
    43621 cgccactggt gttcaaatgc gctctgaagg agaaggctcg cgccctcgcc gagctggagc
    43681 agggagttca cctcctcgta gtagacgagc tcgcggcgaa gctgctcggc gtatcggcga
    43741 cgcttcgttt ccgggtcctt gccgatcttt ttcagatagt ccgcgctgta gtcccacgag
    43801 tcggtccgga ccgtcggggc gccgatggcg tagtgctcca cgatgtccgc cggcgcgact
    43861 tcacgttcga acacgagtgc ctcgagcgaa gccagcagaa aatcgggaaa agagggaagc
    43921 ccgtattttt tcttgagcag cgccgagagc gcgctttccg cgccgatcgt cgcggggagg
    43981 aaggaccgga tgagcccgat gaattcgtgg atggtctttt cggacgagag gttcttcttc
    44041 tccttgtcgc ggcggaccgc atcggtgtcg cgctcgtcaa acggctcctt tcgtagaccg
    44101 tcgagctccc gaaggcggct ttcgacgagc aaaaagagct gctcgcgcgt ttccccgtca
    44161 agccgcagaa aggagaagaa atagatcgcg tagtccttga gttccggctc gaaaaccgag
    44221 cggtccatcc actttgcgcg gtccggcggg acctgcgaga agggcgcgat gagcatccat
    44281 cgcgccagga cgcgcagata gttgaagtgc gtggggtcct ccaccgcggg gtccgcgagc
    44341 agcgcctcca tgacgcgctg cagcaggacc gttaccgtct cttggcacgc ggcgatctcg
    44401 cgaaatacgg cgaagtagcg cggcagcggc accagacgct tccgaaagcc gatcccaaag
    44461 cggttgttcg cgtggatgag caggcgcttt ttaaagagct tatattcgcg gtatttatcg
    44521 tagagcgtgg gtatcgcttt cctcgtgagg tcggagggtt caggtgcgga cgccggcttt
    44581 tcaggttcgg atttttttgc gggaacgggc ttttttcgta gcacttgcgg tttctttatc
    44641 tccacccgct cgccgggcgg cgcgcctcgc ggtttgtccg ggcacgattg gatgaggtcg
    44701 atgatggtgc ggtacttctc gtcgggggaa atcgcgccgt tgttgatgag cgagcgcacc
    44761 aggtccatct tggccggcgg aatgtccttg tcgagcagaa gcgatttcgc ccgccgctcg
    44821 agctttttta tgtcctgggg gttcattggt tccgtttatc gcagtatcag cgaatacggt
    44881 ttttcggttt gtgtcaatga tattatctgc ggggagggca aacaaaaagg cccccgattg
    44941 ggggcctttt tggaggaaat tcgtattggc ttagtaaagg ttgaaggtca gcgatgcgcc
    45001 aataaaaacg tagtggacga tgctggtaac ctgtgtgcga tcagccaatg ttaaaccatc
    45061 tggatcaaaa ttcggattca gatttgtttc acaaacatat tggttgataa atgaaacgtt
    45121 tgatgcaagt ttcaatgagt aaccgaggta ggccctattg cgatagtagc cgatctttgc
    45181 gaaaccgcca tcgccatacg ccattcggga ctcattgttg tccctgttgc gttcagtggt
    45241 tttattagga tcttcagact gctcattgtc gggaacgaaa cgggtttcat tgtcggcaat
    45301 cgtaccgtag aagatttcat agcgggcgaa aatggttgct gtcttcgtga gtttgtattc
    45361 aagaccaacg agcgcacgag tggtcatggc ccatccatat tcttcgctct tgtcccagcc
    45421 tctacgatat gatcccccgt ccgtgccgtc agcatttgta cgcaataccc aagtctcggt
    45481 gtttccctca aagatctgat tataaaggat aatccgagcc ataatggtga aatcaccaat
    45541 tgtatacgaa ggaactgccc atacgttgat cgcatgttca tacgggtagt tgtttctcgt
    45601 attgtgaatg tttttactgt cggattgtgg agaaccgttg aaataccaat cggcaccaaa
    45661 ggttaccttt aatgaatcat cgacttttaa gttatagagg aaaccggtat acagctcaag
    45721 aaaccaaacg cctaacggtt gcccgttgtg gctagtctga gccgttttgg gatcatacgt
    45781 atctcttcta taatgttctg tccgaacatt aggttgtgtg aacaatccga attccgggct
    45841 taacatgaat ttaagatcca gcatctccca tgtacggtgc atatcctgta cgcgagcacg
    45901 gggcgcatca gcaaacacat tcgtcgatcc gatcgccaga atggctgcca gcgcgattag
    45961 tacaagcact ttgaatttca tacgcattct actctccttg ttttatttca taaaactttt
    46021 tgcataaata aaaagggtgt ccccttttga tttgcgtgga aacacgaaga catgtcgcaa
    46081 cgcaaacgcg ggaaacccta ttttctcgca tggttttgtc aagaaaatat ataaaaaaaa
    46141 taaagtatgc acttaatcat tttcctcgca gcgcgcgtgc atcgatatcg tcggatatca
    46201 atgcacgtag cgaggattgg ccccggaatt atccgttacg cgcgcgccga ccggcgccgt
    46261 tccagcagaa aacgtatcac caagacgaca acgccgatgg cgagcaaaat cagcatgata
    46321 tcgcccgccg tgagggcgat gttccccgac gggatatagc tgaacaccag caggtagatg
    46381 agcgctgcga tggtggtcgc gaacatgaaa atccccggga tgaacgcgaa catatacctc
    46441 ttgccgcgtt ggaggagcca catggacacc gtgatgagcg tgagcgccgc caggagctgg
    46501 ttcgcggagc cgaagagttt ccacagcgct ccaaaggcgt tgaagtaggc gagcaggaac
    46561 atgagcgcca cgcagagcag cgaattgaac agataggtgt tcagaagttt cgggacgcgc
    46621 gggaaaatgg cgcgccagag ctcctcgagg agatagcggt tgaggcgaac gcccgtatcg
    46681 agcgtcgtaa cgacgaagcc ttccaccatc aggatgccga agatggtgcc gatgatcttc
    46741 ggcatgccga aggcgctgtt tagcattccg cccaggccca gcgcgaaggc gaggatcggg
    46801 ttcgacttcg tcggatcggc gggatggacg atgcccacga actcatcgaa tttaaggccg
    46861 tagcccacgg cgatgaccac gcccatcgcg agcagcccct cgagcaccat gccgccatag
    46921 cccacatagc gcgcatcggg ccgctcgttc tcgagctgct tggagctggt gccgccggcg
    46981 acgagcgcgt ggaaaccgct gatcgcgccg caagcgacgg tgatgaacag cagcggccag
    47041 acccagccca cctcgctcat cgcggccgcg gcagcgatgt tgttcgccgg cgcggcgatc
    47101 gtgccgccgg agatcccgat gacgaccgtc gccgcgatca tcaacacgac gccgccgtac
    47161 agaaggaacg agttggtgaa atcgcgcggc tggaggacca gccagaccgg gatgcccgcg
    47221 gcgaatatcg tataaatgga tagaatgatc tgccacacga aggggttgat ggagatcggg
    47281 tgcaaaaagc cgaccagcac ggagacgacg cacacggtaa tggcgatcac cgatcccagg
    47341 agcacgctga tgccccgctt gtagagcagg tagcccagga gcggcgcgca gcagctaatc
    47401 acgatgaccg aggtggacgc gatcccgccg atcaccgcat gcggaacgcc gttcacatcg
    47461 accgtgcgaa cgccctgcgc caggtcgctc gggaaatggc tcatggggta catcgacgag
    47521 agcgcctttg ccgtggcggt gagaaagacc gaggtgacca tgagaagcat gatgaccgtg
    47581 aagccgatga acagcatgaa tccggtgcgt cccagcgttt tttccgcgat ctcggccatg
    47641 gagcggccat tttcgcggac ggaggccagg agcgcggtga agtcgtgcac ggcgccgatg
    47701 aggaaggtgc ccacgacgat ccagagccat accggcaggt agccgaagat gagcgcgaag
    47761 gtggggccca gaatggggcc gccgccggcg attgacgcga agtgatggcc gaagagcacg
    47821 aacttcttgg tgggaacgaa gtcgatgtcg tcgcgctgct cgtgtgcggg cgtggcgtgc
    47881 gagccgtcca catcgaagac cttctcgatg aagcggccgt aaaagcgata tccgataaaa
    47941 aagacggcga cggtgatgac gagtataagc aatacgttca tggatgctcc tccgggcgtg
    48001 gcgtgtacga catgctgcgt tcgtcgtcat tacggggagc ctgtgcgaag aggcgaccgt
    48061 ccgccacccc cgagtgccgg gaggaacatg tttcctggac cgcgccggac cccggatgcc
    48121 gggtcgggcg gtcgctgcag gtccacatgc ttcgtgcgca atgaccgtaa tgcaccggcg
    48181 cggttttcgc gcggatgcgg tacgtcaatg ccggtatcat gcgatgcgcc gcggcgctgt
    48241 caaggaaaaa tatacccact ccgtcctgcg gacaccaccc gcgtcgcaag gggaaggcga
    48301 gagggaagaa gtaatcggta tttcctaggg cactcctccc cctaacgaag ggggaggccg
    48361 ggagggggtc agcgccggtc gaggacggcc agcgtctcga tgtgcggggt ctgcgggaac
    48421 atgtcatagg cctcgaatga cgtgaggcga tatccgtcga ggcgcaggag gtcgtccttg
    48481 aacgattccg ggttgcacga catgtacacg atccgctcgg gggcgagcgc agcgatgcgc
    48541 ttgacggccg cggggtgcat gccggcgcgg ggcggatcga gcacgagcgt ggtgcagcgc
    48601 cccgtaaagg ttgcgagaaa atcggccgca tcgccgtgga cgaaggaggc gttggcgatc
    48661 ccgttttgta cggcattggc gcgagcggca gtgaccgcct cctccacgat ctccacgccg
    48721 gtcacggtct cgcaggcgcc ggcgatgaag agggtgatgc tgccgatgcc ggcgtacagg
    48781 tcgagcacgt ggccgcgcgc atgctcgcgg atgcggcggt acatgcgaag cgcgatctcg
    48841 gagttggact ggaagaaggc gttgggcgca atggcaaagc gcagatcgtc caggcgttcg
    48901 tcgatggcgc cgcccttcgc gtgttcgagt atctccccga agctcaggtc ggcccgtccc
    48961 gcgctcagga gtatggagat cgacgccacc cggtcggcca tcgcggcgat gaccggatcg
    49021 agacgaccgc ggccgtcggg cgaggccacc acgaaattga gcatcacctc gccggtgaag
    49081 cgcccctggc ggatcaccac gtagcggaga tagccggcat gggcgaggta gtcgtaatcc
    49141 tcgacgcccg cgaggagctc gcggacccgg cgaaaggcct cgtccgcggc gtcctgctgg
    49201 atgcggcacg agccgatatc gacgacgtgc ttgaagctcc ccgcgcggcg cagcccgatc
    49261 ttcccgaagg cggtcacgaa gtccatgcgg ttgcggtagc cgaagggatc ggccggctgc
    49321 accggaccga cgtcgatggc gccgtcgaag agcgcggcga gatggtcgcg cttgagcgaa
    49381 agctggctct cgtagggcag atgttggagc gcgcacccgc cgcactcgcc ggcatggggg
    49441 caggacggat cgacggcgac gtcggcgtat cgcctcgcga gttcgagaaa ggcgcgcttc
    49501 ttttttttca tgatatgccc agtgcggcga ctgattttat agcctggagt tatcgcgcag
    49561 cgaaggattg cccgcatgct ggatcatgca tggaaagatg cgttcgccgt aaagaaaaat
    49621 aattgaaata atatcgaacg gggggtccaa tggatggcga ccgcccgtgg gcgggagtat
    49681 cgaggagcgg aggtcgggcg tgattatcgt aaccggcggg gcgggattta tcggcagcgc
    49741 cttcctgtgg aaactcaatg agcaggggat cgacgatgtt ctggtggtcg accggctggg
    49801 cacgtccgac aagtggaaaa acctggtcca ccggcgctgt atcgacttca tccacaagga
    49861 cgatttcccc ggcctgctcg acgacgggaa ccgctatttc agggctagcg ccatcattca
    49921 catgggcgcc tgctccgcga ccaccgagcg cgacgcggat tatctctacg cgaacaacta
    49981 tcgctacacc cgcaccctcg ccgagtggtg cctcaagcga aacgtgtttt tcatgtacgc
    50041 ctcgagcgcg gcaacctacg gcgacggcgc ccgtggcttt tccgacgacc cggccggcat
    50101 ggatgcgctc accccgctta acatgtacgc tttctccaag cagctcttcg accagtgggc
    50161 gctgcgcaac cgcgtcctgg accggatgac ggggatcaag ttcttcaacg tgtacgggcc
    50221 caacgagtac cacaaggcgc acatggtcag catgatctat cgcgcgttcc atcagatccg
    50281 cgagaccggg cgggtgcggc tgttcaagtc gtacctgcag cagtatgccg acggcgggca
    50341 gctcagggat ttcgtgtaca tcaaggactg cgtggatgtc atgtggtggc tgttcaacaa
    50401 ccggagcgcg agcggcatct ataatctcgg gacgggaacg gcgcgctcct ggaacgatct
    50461 cgcgggcgcc atcttcgcgg caatggggaa gaaaaccgac atcgaataca tcgagatgcc
    50521 cgaggagttg cgcgaccggt accagtactt caccgaggcc gatatggcgc gcctgcgggc
    50581 cgcggggtgc aatcatacct ttatgaaaat ggaagattcc gttcgcgatt acgtggttaa
    50641 ttaccttgca cgggacgttc gatgtttgta ggccatgaag cgttttctgt tttcgtatgt
    50701 cgggccgtat gtgggcctgg cgattcttaa aagcctgacg gcgacctatc ggatccggat
    50761 cgtcgacgag gagaacgagc agtcgattta ccgccgcggg agccgcccga tctattgttc
    50821 gtggcaccag cgattttttc cggggatcgc gtttttcccg aagcgcaaca agatcgcgat
    50881 catgatcagc ccgagcgcgg acggcgagct tatcgcgcgc gtcgtgaaga tcctgggctg
    50941 gcacccgtcg cgcggctcgt cgtcgcggcg cggcaaggag gcgcttatgg aactcaggcg
    51001 cctggccgcg gagcgctatt ccatcgggca catcgtggac gggcccaagg ggcccttcgg
    51061 cgaggtgaag ccggggctca tctcgatggc gcagcactcg gggctgccga tccttcccac
    51121 gatcacctcg gccgagcgcc gctgggtgtt cgggagctgg gaccgtttta tggtgcccaa
    51181 gccgttctcg cggatcatca tccgtttcgg gaaggagatc ccggtgccca gcgagatcgg
    51241 cgacgcgcag ttcgaggagc tgcggcggca ggtggagcgg acgctgcggg agctctacga
    51301 ggacaccgac cggatgtggg agcgcgcgcc gcaggcgtcg tgatcgaact aacggtgcgc
    51361 atgagttgaa ttgttgcacg gcacgtacat ataaaatcca agcgaaagag atgattgtat
    51421 gagtacattg atcaacatcg gcgtagcgca ggtgggcacg gcgtcctcgc ccgcggtttt
    51481 gcgcaccatc ctcggaagct gcatcggggt gtgcgtgtac gagcgcatga agaagatggg
    51541 cggcatggcg cacgtgctgt tgcccagcac ggtgcagggc ggcaagccgg aaaagtacgc
    51601 cgatacggcg atcccgatgc tcgtcgagcg gctgctcgcg gacggctgca agaaggaatt
    51661 catgtcggca aagatcgccg ggggggcgtc gatgttcacg ttcaactcct ccgtggcgct
    51721 ggcgcagatc ggcgagcgca acgccgagat ggcccgcgcg gtgctcgcga aatacgggat
    51781 ccccatcgtc atcgacgaca ccgggggcaa tgcgggacgc gtgatcgact tcttcctgga
    51841 ggacgggcgc ctcaaggtga aatcggccgg cgaggaaaag atctactaca agctgtaacg
    51901 gacatccgcg caccggaggg agacaatgaa cccgcagata aagcagagaa tcgaatcggt
    51961 catcaacaac atcgagcagc tgccctcgat ccccgaggtg gccacgcgca tcatcaacat
    52021 ggtgaacgac cccgaggtct cgttcaaggc catcgccgac gagatttcga aggaccagat
    52081 gatcaccacc aacatcctca agctgtgcaa ttcggcctac ttcagcaagg gcaaggagat
    52141 ctcgtcggtg gaccgcgcga tcgtgacgct gggcctcaag gaggtcaagg acgcggtgat
    52201 cctggcggcg acgaagatcg tgctcaacaa gatcatcatg ggctacgacc tggccaaggg
    52261 cgagctgtgg cggcacggcc tgtacgtagc ggtcctggcg aagaagatcg cgctcatgaa
    52321 agggaaaaag gacattgccg atgtcgcctt caccggcggc ctcatccacg acgtggggaa
    52381 gacgatcctc gcgctcttcg tgcagggcac cttcaaggag atcatgcgga cggtcgagga
    52441 gaagaagatc cccttcatcg ccgccgagaa ggacatcatg ggtttcgacc accaggagat
    52501 cggcgagcgg atcctgagca agtggaagtt tccggaggtc ctcaaggcct cggtgcgcca
    52561 ccaccatcag cccatgagcg cgccggatga gttccgtacg gtcgtgtcca tcgtccacgt
    52621 ggccaactcg ctgtgcctca tggccggggt cggcatcggg agcgacggcc tctatcacga
    52681 gcttgtgccc gaggccatcg agctcacgaa gctcgagccg ggcgagctcg agatgctctt
    52741 cgccgggatc ccggagatgg gcgaacaggc cgagacgctc atctgagccg acggatgcat
    52801 atcgtcaaac gctgtcccgc ctgcggaaaa aacctccgct ttcccgtcga caaggggaag
    52861 atccgcgtca cctgcgtgtg cggcaacgcc ttcaccgccg accccgatga tccgcggctc
    52921 ttcgacgggg ccgccttcga catgggcgcc caaaagaaac ccgcgtcgcg ccctgccgcg
    52981 agcgtgcggg tgcgcgcgct cggtgatgcc gtcattcagg ggctctactc gtttacgtac
    53041 cgcgtgcaga atttcaggct tctccccacg agggaaaagg cgcaggtcct cctcgtggtt
    53101 gtcagcgtcg cggcgctcgt cgcggcggtg ctcatctact tcctggtgaa tcccgcgcca
    53161 cgccccaata gttcggtgat ctaagcgtgc gtctcaacac caccgacggt tactatgcgc
    53221 gcgagatcga atcgctgggc tgggagctca ccgtgtgcaa catgctcgcg cgccccgaga
    53281 gcccctgccg gggggtgctg cgcgtcaacg cctcctacgg cgccctgctc gccgatttcc
    53341 tcgcgacgct cgtcccgctt gcgtcctgct cgtcggtgct cgaggtgggc ggtggctacg
    53401 gcgtgttgat gcgcgatttc ctcgatcgcg ttccgcacct cgcgtgcacc atgctcgata
    53461 tctccccgtt tctgctggaa cgccagcatg aggcccttcg cggccggtcc gcatcgttcg
    53521 tggcgcgtga ttttttttcc gttgaggacg cgtgtctcgc cggtttcgat tgcgcgattt
    53581 taaacgagat cgtcggcgat ttcccgacgg cctgcgacat cgatccggac gccctgtcga
    53641 acggcaccga cgcggatgat ccggccacgg cgcgcgtgcg ccgcatcatg ctgcgctatg
    53701 gcatcgcgcc gccggagggg gcgcgattca atttcaatct tggagcggcc gaggcggcgg
    53761 agcggctctg cgcggcgggg gtgccgtaca tcttcatgag cgagcacagc tgcgaggcgc
    53821 gcgtgccgga tgcgctcgcg gggctggtgt cgatcgcagc gcccggcaac cccgagcgca
    53881 tccccctgaa ggggcacgac gaatacacga tcgcgttttc ccatctcgtc gcgatcgccg
    53941 taggccacgg ctatcgcgtt gtgcgcggtc cgctcgccga catcgtcccg gtcgatttcg
    54001 acgggcgcgt gcgtttcatc atgtcttcga acgcctcgcg ctcggacgag cacgagatca
    54061 tccgccagtt cgtcgaggac ctgttcaagt acgaatacgt cgtgttcgtg aagggctgat
    54121 cagtcccaca tcttcgtcgg gtctttgggc gcgccggcgt cctgcgtccc gcctttcgct
    54181 ttttcggcgc aggcgcggca gagcccgtcg tcggagcagg acgcgcacgt caatgcgccg
    54241 caggtagtgc agggcgcgaa gcggctgcgg acctcatcga aggcctggcg tcgcgccccg
    54301 tcctgttgct gctgcatgtt ctgcgcttga cgctgcccca ggtgggtgct cacgagaccg
    54361 ccgaggacgc ttgcgaggac ggacccgata atgggtatgc gccagagcag cctcgacagt
    54421 ccctgctgcg cgccggtcga gaggatgttc tgcagcgtgc cgctcgacgc cgcggcgcca
    54481 taactgttct gtgaaatcac ctgcgcgccg caggtcggac agctgaaaac gtatttcgcc
    54541 tgcggtccgc tcccctgcgg atatcccgat tccatgattc gattgagggt gtcgctcatc
    54601 attgccgctc cgtgtggatg tcgtgctgcc gtcttcccgt cgatacgtat atcgtttcca
    54661 gccgggcgtg tcaagccgga agggcgtgat tgatgcatgg ggaaaaacgg attgacctga
    54721 gtcgcacggc gggataggct tcggcattgc agcaaaacgg caggagggac ccatggcgca
    54781 gaagaaaaag gagaaaaaac tcaccccgtc caccaatgag ttccagggca cggacgtcga
    54841 atcgctccag gtctcgttcg tgaatcatct ggagtactcg tgcgccaagg acgagttttc
    54901 ggccaccaaa cacgactgct acaccagcac cgccctcatg acgcgcgacc ggctcatcga
    54961 gcgctggatc gaaacccagc agtcctatta cgcggcggac gcgaagcggg tctactacct
    55021 gtcgctggag ttcctcatgg ggcgcacgct cggcaacagc ctcatcaacc tggggctgta
    55081 cgacaacgcc caccgcgcgc tcgtggatct gggccacgat ctcgaggagt tgcgcgaaat
    55141 cgaatgggac gccggcctgg gcaacggggg cctcggccgc ctggccgcct gtttcatgga
    55201 ttcgatggcc acgctggagc ttcccgccta cgggtacgga atccgctacg agtacgggat
    55261 ctttttccag agcatcgtcg acggctacca ggtggagacg cccgacaact ggctccgcta
    55321 cggaaacccc tgggagtttc cccgcgccga gtacctctat ccggttcggt tctacgggac
    55381 cgttggtcat cacgccgatc cgtccggcgc gcagcggttc gaatggaccg acgggcagat
    55441 ggtgatggcg atggcctacg acacgcccat ccccgggtac aacaacaata cggtgaacaa
    55501 tatccggttg tggtcggcga aatccacgcg ggaattcaac ctggagtact tcaaccacgg
    55561 cgactacgag aaggccgtgt cggaccaggc caacagcgaa acgatatcga aggtgctcta
    55621 tcccaaggac gagagcttca agggaaagga gctgcggctc aagcaggagt attttttcgt
    55681 ctcggcgacg ctccaggaca tcatccgccg ctataagaag atccacgact ccttcgacgc
    55741 cttccccgac aaggtggcca ttcagctcaa cgacacgcac ccggcgatcg ccatcgccga
    55801 gctcatgcgc atcctcatgg acgacgaggg gatgggctgg gaggccgcct gggacataac
    55861 cgtgaagacc ttcgcctata ccaaccacac ggtgctcccg gaggcgctgg agaaatggcc
    55921 cgtggggctg gtcgagtcgg tcctgccgcg gcacatcatg atcatctatg agatcaaccg
    55981 ccggttcctc gaggatatcg cggcgcgata ccccggcgac atgaaccgcc tccatcggat
    56041 gtccatcatc gaggaggggg ccgtgaagaa cgtgcggatg gccaatctcg cgatcgtggg
    56101 cagccattcg gtgaacggcg tcgcggcgct gcacaccgag atcatcaaga acaacatctt
    56161 tcgcgatttc ttcgaatact ggccgcacaa gttcaacaac aagaccaacg gcatcacgca
    56221 gcgtcgctgg ctcaagctct gcaatccggg gctggccggg ctcatcaccg aggcgatcgg
    56281 cgccgggtgg acgaccgacc tcttcgagct caagaagctc gaaccgctgg ccggcgatgc
    56341 ggccttccag gcgcaatggg cggcggccaa gcgggtcaac aaggagcgcc tggccgaata
    56401 catcatgcgt aaaaacgggg tgcgcgtaac ggtcgattcc atcttcgact gccacatcaa
    56461 gcggctgcac gaatacaagc gccagctcct caaggtgatg cacgtggtcg cgctgtacaa
    56521 ccggatcaag gcgaacccct cggcggccat cacgccgcgg acggtgctct tcgccggcaa
    56581 gtcggcgccg ggctactaca tgtccaagct catcatcaag ctcatcaact ccgtggcgga
    56641 cgtggtgaac aatgatcccg acgtggccgg gcggctcacg gtgctatttc tggtcaacta
    56701 ctgcgtgtcc aacgccgaga agatcattcc cgccgcggac ctctcggagc agatctcgac
    56761 cgcgggctac gaggcctcgg ggacaggcaa catgaagttc gcgctcaacg gcgcgctcac
    56821 cgtgggcacg ctcgacggcg ccaa
//
LOCUS       DAORHF010000003        50843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_301_length_50843_cov_6.563046, whole genome shotgun sequence.
ACCESSION   DAORHF010000003 DAORHF010000000
VERSION     DAORHF010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 50843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50843
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_301_length_50843_cov_6.563046"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..8157)
                     /locus_tag="PKJ16_00585"
     CDS             complement(<1..8157)
                     /locus_tag="PKJ16_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45506.1"
                     /translation="MLPGILSRAMLCLLVAVPLAADTVRLKRYGEVERKLDRSFESAS
                     VKRDEAEWVRVVNAGRDALKADWEREAEAALDRELVARIGTGEDAEAVRVELAELFAR
                     AEAELDAAMRADIEYERGRWLASVAPIAPPRFDHETLLDSIARADALPVGTGLTDAYV
                     AWNAEAGETFAELWGNYETALEAERERALAGIEYLDELRAGFVAGLDERLARMRADAL
                     ADEEIYLIGAKNRFIYRNRYDTDSLRRESEANSASVITRSILDQTRMKIDDLMERALD
                     TGSATYEGDAGVPTVEGYSDRVKAIIDAGLASWRNAEEGLFAARLACEKRAGEDFAKS
                     DRAWEDASEELRARKNEWLTQIQTQLYQGGLVWDNAFDQFDRNYLDAQDELARYLEAQ
                     GASWDDYITTLRGMADTGSAALLNGQESIAWMTDLAARYARKGDGSMTGLLSEEIARW
                     REIMENFERILVNSEMMLHAGDIWGGTIGGFVFSGLLDDRRSDRFYLEYTDDGRVVFA
                     GDIENVRDPYLMTAAEFELEKIKIEEKFWNKRLLMAQAVYDFAYDKAGRASKEDQAAA
                     LDEARVERDHWQAEFDRARERLTSEFLAAVESAKTNYHDAQEALEEAQAEYDVAEEQF
                     RIANEKLLYLENPNAREILLREINDLTETVSDLRRSVAGADDRLIGEARAYYTAVKLR
                     AEAREAKAYAERLRDAIGVLEGAGDSAGYRDGSDRFAEIVASARANGDSFEALAQAVR
                     DNADDIFISIDKNDPYYMDAANEGIARARAALETAYDRHAAFTALVARFDDDPDAERE
                     AIMFELLPGRAGSGVFGEKTLADVMNAFTTLAAGSDAEKNQALAEIAGYSYGAYALTD
                     FSGTTPDEIKSMMLRAWAMAITGKPLVEIAGLSGPALDALLGDAAALEKGAREIELMG
                     AIGDARVAAETAFLTAKGVVTYLLYDFNGVATITDDTENSAARLAARAKDASRFNRYE
                     AERNHETVTALAAYLKEPGKKGMSANELAAGLLVRARERFADDPGGVGPESARLAGIY
                     RFIVQTARKTLDDIAAMTNAAERNDAWDAFMETISFEERRAGYIRDLYATEIMPDATP
                     QQVMERVTAWQRELATGKDADGNVLSDTDIERRGAYVNALTSGQGALLFSDPRLVAAE
                     GADYAVKAALAGYAALNYDASVERRVKDRSGVLSRIAAAIGKTVEEVKNIFIGGQSYE
                     ALAEVGEALRAYRKSDEYQRLPVAVREALAAAESKYIDMLKRDYVLQNRALSEGEAKA
                     RLDTIVSQVETLDQVMAAYGEALAEQTKDYGEEERAFYSRNRYALYTKKIHETVRALE
                     ARIDDLAQLANFTMRVEGSANIISAIPPLIASLRAKRFEYETQFYMYEYLAHSFAYGN
                     SVAAFRERLLLTEGDAAFARIDEFTDDRAGFADEVAARLRTLDLRLKVREALGAGALP
                     EMADTVGYWLNGFLAAAGVDPASLADDEAADLALFARLTYYDRLNASVGGGGFWADRL
                     ASDFRDYGYLLEFMAICAARGDAIDDTPFEDLFAALVGKYAAQADRLDARRGSFEEIY
                     DAAIAGRDVFIERELSGDVVATVLRGEYFADFFASKGLLMTVDDFLVGMGVAESDPLY
                     GSFRDDLGAYRERLSIASGYEYGADIEEFIERRGIIGDSDRDFLRRYAFSPDGASPYA
                     IMELTNRFDLKMASDAATRDFAALITTLYGADMKGGEYRTTADYAEDMGLLRAKATSL
                     YDYTRLGPDAFDNFRSSWDTAFQNWDYYIGKFVADGNDDRFVVLKPGEVPSAEDAEKP
                     VITKISDVEYLIPDEVRDAYLNATVGDPDKNIEVKWRYPIEYQELVGLLLSDKMGERF
                     FHRAGEDGLANSLIDAMTEYNRALYVMYAVSGIEEDALAAASLAGFDNLHGLSPVEDL
                     LAVMGANYCPDTMNPYGADMDDAVRAEKLAAIDTLTAQIENAHTAWIATGDSDIVAKA
                     RDEYLEDVTKYESAKKKIERVAKIQKGLEMTASKYVTTDDPENGFDYVRAKRVHQEKQ
                     AAYDMAQAERAAREAIYRTAQGNYFSQLNAIAEIYRRLQAAIDVYDTEKAVYEYAATA
                     YLYNTSTNADDKRGPEKLMQDTKQELDMVQGIHNEVLERMARQQEEVNRVKEQDVLTD
                     AEYVARMEELKEKSQRYFRLAKASYLIDKEVQKRTFEYEQARAAYESYRDTLIAANGD
                     ENEKKRNEFLDYFMTRDMYVATWRPAVTQIPGLGHIDWLSWVLPAMTHDNEFVDMAYY
                     YNSNKDAIFRTPPYWIGSEPSGSDFYGFMENSWDNVFSGGELTGAMTTFATEMLRHKE
                     EAAQWTRFLIVGGAGAHLLLANWAAKTALESAINAVQNQINRIPRRMRWMADPLKQLL
                     NALRGTLNQVVSSLVNFITMDMGSHYRAANNHLSNAQSAVNNINAELTELRNRKDRMQ
                     KAMVKLAEITQIETLDDDANAGVITYRNPRGKWITVRKRSLKTVIKELGGTNGFEISD
                     EDLAYIMEGAQGDANIFDAAPARENPSVVFDENGEVCDTCDKTGVWYYNSAPVTSKFT
                     AHMNQRRLHDMKEYFDYTAKMTSPPAAERFSDILSALGTATSCWEPLPGGEGSPKYDS
                     VVVWRAKENLFYEIATDAQANGYYSALEAGRAYTGYRKAMTDYYGVLEGFSFDDMREL
                     DMLIDEGAPGLAERFAALVGARTGINDAELMQRQALQNYEWQKKREHRDYERARWEEK
                     AADIFER"
     gene            8310..11477
                     /locus_tag="PKJ16_00590"
     CDS             8310..11477
                     /locus_tag="PKJ16_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV45507.1"
                     /translation="MTAGISVTRPIAVVMVYLAGMVFGAIALSKMEVDLLPRVEHPEL
                     SIITLYSGASPREVESLVTRPVEEAASGVPGVRRVRSESIEGASLVTAAFEWGADMDF
                     ATIKMREKVDLAKGALPQDAEKSIIARLDPAAQPVAGIAIRGRDGGVDGLRQMVHAKI
                     KPLFERIDGVACARIAGGRTREVQVRVDQSRLYANGLGLRDVIESVRLSNYNFPAGSV
                     TRGAREFVIRTIGEFASLADIENVLVRKSARGSAVYVRDVAEVVDGHRDRDSISYHNG
                     AESIGLAVVKEAGSNTVRVCAAVRDLVSEINARHRGAIELAVCYDGSRYIKNAVDNVV
                     DAAVLGAVIAFFVILFFLRDVKSSLVIIAVTPPSVLITVLAMYLRGIGLNMMSLGGLA
                     IGVGMLVDCAIVVIDSVHARLAAGEDLRAACRGAIAQDRAALFVSTLTTVVVFFPVVF
                     VRGIAGQVFSDLAFTISVSIVISFIVSITLTPALLVLLERRSLQSGGMMGFAPLRRLA
                     NIPALRRSAERLRAALEWAHSLLERSYTAYLVRLERRIDGYRRALIIGVTLVIGGFLM
                     FAFLDTSLFPRTNSGSFSIRIVMPPGTPIDDTREAALRVDAALAALPFVENRFIDVGY
                     NPGEISEYFGREKSRATAELVVTLKRDRGVSTEAAMRIARRAVALPPEIAVEFFSEER
                     EFAGFMRLGSSTVLVDVTGDDFDAIEQSVAVLTAAFEKTPGIARAVPHVAKGKPEVRL
                     VIDRAKLAAFGLALGDVAGTVRAAVRGEDAGKFYEADDQADISVRLRAADRSGLASLG
                     GMLVRVGESANVPLAAFSATERQSGYNKITRRDQRRTFSIALDAVPGTGRGAAIAAAA
                     GAIERVRLPHGVHAAVSRDMDEVRDSLAELAFSLALSMLLIFMILAAQFESLTRPLYI
                     MLIMPLTAAGVSLPLLFTGNSVNVISLMGTVLLSGSAVNAAIVLVDYIQARRAGGADL
                     RAAVIGGCRERLRPILMSTCTNALGIVPLAIGLREGGEMQSPIAIVFFGGIAVSTLCT
                     LALLPAYYYHYESRAERAGKRSANG"
     gene            11579..14716
                     /locus_tag="PKJ16_00595"
     CDS             11579..14716
                     /locus_tag="PKJ16_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV45508.1"
                     /translation="MREIVRFFITRKITTLMLFAAVLVFGCLSLARIPTALLPAAAKN
                     RVTIAVRYPGQSPQRIERIIVMPIEEAVSAVGGITSMVAACKEGEARIHLAFAEGADM
                     KYTTVQLRERIDPVRASFPRDADEPEIYGASADERPVLIVTLRSDTLAIDALREIAEK
                     RYKKLFERIEGVSEVAVGGGVKREIEIAVREDRLVSHGIGFDRVMRAVQMNNVAVPAG
                     TIGERREHALVIDGKFRTLDEVRATPILATAAGSPVFLGDIASVRDYAGRREELSRRN
                     GAENVSLYLMQTGAANCLELSRAVREALRAANDPRIHIDIAYDQAEFIGRAIRNLAIS
                     CVTGIALASLVLFAFFRSAREALPVMLAIPFSLLPVFSYLYFSGMTLNVLSLTGLALA
                     AGMAVDSGIVVLDAINKRTQEARGAARNGLLSPDAMNTICLDATADVAGPIFASTVTS
                     VCVFVPMALFGGPALGLFRDMAGAVIVALCSSFFSALTLIPLAAALAHRFDVALIINT
                     LPFGALRRLARRVANARPVAAVHAIALRIVARTIAYRTALPQYEVLMERLLRNRVRAA
                     LSVAMILAALLFLSSFLREEHLDPLESNELSATVELAAGTSLEETARVVGNIERTIRA
                     ERAVRELSTKIEKGRADLIATLRDGADKRRTARDLKRRLAGTTDAFVFFQEERAGESA
                     RELDFEIIGADVERIRDIARDLAARLSALGGSEDVVLRFKEGRPAVEIVVDSGKAMES
                     GLSAGVIGDFVRSSFHGPVASKFVDAHEVDIRCRLQADEARVIERLPALPIITAKGAA
                     VPLGELASFRNASAITTIWRKDRARMETLTVTLGDTDLERFVKRADAVIAAMPLPAGY
                     RIDYGDSLANLRRSKKELLGALALAILLVYAVVAALFESTLLPLAVLAAIPLSLIGVL
                     AALVAGGRSLNSSVYMGMIILVGIAVNTAIVLIDALEKERRASGHIDFPAIVSVARRR
                     IRPVAMTTLAATLGLLPLLFGGSGSSLWRSFALAVIAGLAASGVMVVYAVPIAYHAVR
                     GGRQMSRMVSKSDAG"
     gene            complement(14677..16305)
                     /locus_tag="PKJ16_00600"
     CDS             complement(14677..16305)
                     /locus_tag="PKJ16_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV45509.1"
                     /translation="MLQILLRILFIAMMTALISCSGADPIDDALNITVPEMDNGAVHP
                     SNGASGVEPRPAISVTFPREMDRPKTEEAFGISSEYGVPRGYFRWDGNRLHYDLIEDL
                     RNGATYTVRVSRGAEDLRGNNIREEIVAHFSVGSDLEKPRVTAVLPADGSIGIARNAV
                     VRVEFSEPIALSSAEAGFSISPGVLGTRTLSDDGRTVLFTPYAGFENKTGYSVIVGRD
                     ITDIAGNPLLEEKRFSFMVGGDFAVPTLDPARAVPADEGVGVFAVPPAGAPLRLDAGN
                     LTGGVEKDTALRIVFSKAMKPLEAGDAVRITPAPSGGHRITWTDARTATVSLSGGALE
                     LGRVYELEVRDSAIDVADNRLDAAYRFSFKINGPHSQAIFVQDIRQMEWLPNDDPALA
                     VVDESKAGRSVFGCYGIIDLDVYNSELPPDLRRYWGFMHRINDTMKAVYVLRVYFQNT
                     ANSLQGIGMSWGAAQRAISFAWDRYHGPGTHALMPVTWRMERPAAAPNAIDLYLFDLE
                     ADNYFRLRVAGGSDGMRDALGNYPASDFETILDI"
     gene            complement(16321..17775)
                     /locus_tag="PKJ16_00605"
     CDS             complement(16321..17775)
                     /locus_tag="PKJ16_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV45510.1"
                     /translation="MLMRLNSNRWIVRVMACAAIACAACSQDVAPPAIEGVSPGHQAS
                     VRADAPVTVDFSEPMDRERTEGAFSLTSSEGMVAGSFRWENDDRRLVFLPELPLANGR
                     YYTVRVTTAARDRAGNALTVEYLSSFLVGDDLAPPEVVSISPENLTRAFPLDGVIRIE
                     FSEPMDRMSVERGVRFTPSIPGTFVWTSDTVVEYRPFAPLVFATDYQITISTACTDLA
                     GNGLLATVESFFTAGDEFERPRITSIVDMSSGTDLAEHGSFHEYNGISHAASLQVRFS
                     EPVDRASFRDALSLSPSAEHQVEWNDASDGCTISFPRGLVSLQRYELRVNRTLRDTAG
                     NEMAEVFVVYAVIDAETSRPPAVAGVWFMDAPLADEVADDYYNDLGVEFSSDSTYQFR
                     VAFDRDVDRVSVHDGLFCSFVNGEQDDTSAIVRSYRWDAALPYSVLTVTLGSIEGGNL
                     YLLEFKGGENGIRGVTGVPMAATRRYFFKFNADD"
     gene            complement(17756..19192)
                     /locus_tag="PKJ16_00610"
     CDS             complement(17756..19192)
                     /locus_tag="PKJ16_00610"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV45511.1"
                     /translation="MKMKGMHAVFRFLAARKKVVFAAMLAVMAAGTAATVFQGQMAER
                     LLSRKTVERDAAPEIATAVIARRDMEHRLSVMGTVAQRDKAAISSKVLGRVERLFAEQ
                     GDAVRRGQPLARIETLPLEMQLKSARAELAAVEAARRLVKEKLDRAGRDVERHMATIR
                     KTVIDGRDKYVSFENMRSVMRKKEELHLVGGVSDAELDSVKTTYNSYLAKLLSAKKDH
                     EILRVGYRDADLMKAGLPVPKDPKEKHALLTKLNTKVERAELDAAEQAVKKTETSIEI
                     LQTSIKEAVIRSPIDGIVAARSVELGERVKDETTLFVVMNTRQVYVVANVNEKQVAGV
                     RKNQPVAFTVDAVADTPFAGRVHLVSPVLDVATRTVEVKILADNRTGQLRPGMFARAV
                     ITMEVLKGIVTVPKEALKDVKDGDGEVFIVKDGAVFRKKIVLGREFEDSFEVKSGLAE
                     GDVVASGGVKMLIDGMKVTEKKNADATK"
     gene            complement(19189..20562)
                     /locus_tag="PKJ16_00615"
     CDS             complement(19189..20562)
                     /locus_tag="PKJ16_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV45512.1"
                     /translation="MTKTAMGAGGVSARRRVCAAAVALLLIAPTSGARAQAAELSLDE
                     AVARALTCSTRLAALKSSIDVSRRIINEKWRSYLPDVGVSYSKEDTVAYREDDYRSQS
                     ILLNVGVDLYNGGRTSAEYRIAKLEALLAAEEYRIERNAIVLNVRSLYYELLKNLDEI
                     AINRKLMESLELQRVQVEARIAEAEYGILKSENEYQNRLKDLRVLIGLAPGAALALTR
                     MEIASVPVTAITRGRDELVAIALAKRNEFKKSTYLLYKTGREMELARAYYLPTIRLTG
                     SYGYTGNRYPPNKKMWNVGISVTTAIFGSSLSGAQNYGESNNGGTRSSQRSGQAGLYD
                     NPSYASAIIRTEASHREARLAHDLLRKSIAIEVVRAHDSVRENATRIAVSEKNVALLE
                     KQVAIEDAKARLGEITRYDVLKTYLDLSSARLRRLVAKTDYLIAVAVLERTLGYDIGK
                     LSGAHAP"
     gene            complement(20577..21083)
                     /locus_tag="PKJ16_00620"
     CDS             complement(20577..21083)
                     /locus_tag="PKJ16_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45513.1"
                     /translation="MNTIHAIRIAGIFLLGLAVSCSSIAIDAVDEGGARTRQIDSLFL
                     GAVEVKSDEMTNARESLMRTMGFMLKKRAFRAGTALDAGDRAEQYPFHAALSVFVVKG
                     GDVLEPRTASVAFFRVVDAKSGAAVITIKAASDSCDLAHPGDQAALCATIAERLDAAV
                     KGPAGWFR"
     gene            complement(21080..21670)
                     /locus_tag="PKJ16_00625"
     CDS             complement(21080..21670)
                     /locus_tag="PKJ16_00625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45514.1"
                     /translation="MIRAGRVMLIVLLACSCGGGAGADERFAHCLALYQKRDLAGAER
                     CLRECVSSRPGHVEAWRYLAMANFHLGRDREFAEAVEQYRTRSNDALDALRLEARWHI
                     RAKRPDEAKQLLERALAGSPDDIVSLYLLGALHADAGRTTEALAAFERALGQYAYLSR
                     IHEAVGPLYAHMGLDDRARAHRAMRDSITRWMEERK"
     gene            complement(21667..22458)
                     /locus_tag="PKJ16_00630"
     CDS             complement(21667..22458)
                     /locus_tag="PKJ16_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45515.1"
                     /translation="MNRLVRSFALAPLAAVAWLACASGGAQVAKRPEPESIITTSGIT
                     AHREFRKKDCAKAPSFDGAIAGVKRIAIRFAAPRDGRNNGGGPRHADWALELQTALLR
                     AGYVINDEKERDEFRFDDEGEKVEVKSDAYLCIDTVEVRELSLTHAVSVVVAGEAMAA
                     EHPYRLHVGALSGRLVLANNDVLWSGSVCVTSLDLAASAEAPAVSATATRAYRFSEKL
                     RAWVADKWELATRDNFSASFADWQADHHRRALIRHAVNEFLGGLK"
     gene            23014..24522
                     /locus_tag="PKJ16_00635"
     CDS             23014..24522
                     /locus_tag="PKJ16_00635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45516.1"
                     /translation="MKIIGLERAMPFAAVMLFLLCLAHPIHGKSAEDYFSDGAIKASE
                     GFTKEAISNYSAAIEINPGYTKAYYNRATAFLRLDRIDEALRDFNKVLSIDNQHHRAY
                     YQRGRISYLKEKYADALQDFNRTLEIQPTDEDALHYRGCVYIVNLELLKALKDFNTVI
                     QLNPRLSLSFAYHDWILYSRDKGRIFFSSFFNRAIMNRITGDRDSANNDIQLINDNFK
                     RIRTIFGDKQADTSLTDRITVALDVYQRVVSAHPRSAQSYQLRGQAYALLDLCDKAIR
                     DYSTSITLNPKLAAAYLGRGYAKTACGKPREALEDFSRAILIKPKLSEAYIARSLAKQ
                     KLGDLKGFLDDIARAISVSPKSIRARKIRGEHYQTTGNLANAIKDYSALISINPDDNW
                     AYIKRAATQSKLGLHEKAIEDYSKLIEMQPTLPYAYFYRGKEKAHLGDKQGSLDDYSK
                     AIQIHPDFKEAYLERGTIKAANGDIKGACADWNKARRLGLTGDIDEKLKTCK"
     gene            24559..25311
                     /locus_tag="PKJ16_00640"
     CDS             24559..25311
                     /locus_tag="PKJ16_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HNV45517.1"
                     /translation="MTPPAVTAIIQTRMGSTRLPGKALLPLAGLPLTAHVIERTMRIA
                     GVGQTVIATTTGAEDRPLMELAREMGCAAFAGSVHDVLERYYLAWREFGGDIIVRVTG
                     DNPFTDVAYNSQAVAFAIETGADLCSVAGIPLGTAVEIFTADALARAYEESAEPHQRE
                     HVSPFIKEHPERFRIARKQIEIDNPFPGLRLTIDTPEDFELARSLYEALYRDGPFPLE
                     AVIAEIARRPELAALNAGITQRAMTHSEHGTR"
     gene            25298..26230
                     /locus_tag="PKJ16_00645"
     CDS             25298..26230
                     /locus_tag="PKJ16_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45518.1"
                     /translation="MGRDNRTIAIVTAASPALGSGHLQRMTTLLWHLVEVSDLDAVVV
                     AREVGPFFPDPLRPYLRASLPDRAALIIRDMRDSTEDDIEHLARRGPVLAIDDAGPGR
                     ALADCAIDLLPNPTTGASEPEAFIYGWGYVDSLRALAAKDASDIARDIDCAIYPGAFA
                     DEARIERLLGLMPAGATSAVLRGAGSFVFRTGAERRPAGTCPYARLLLSSRALLSHFG
                     VALYESHAAGCRPLALNPTAYHARLADLARTRLPVENLGVDGDDDDALLRRRIDAALA
                     APSPRAVSVHEAFDAVMSRLDAFARIVRTLAGTR"
     gene            26302..27192
                     /locus_tag="PKJ16_00650"
     CDS             26302..27192
                     /locus_tag="PKJ16_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HNV45519.1"
                     /translation="MNRLMLFGLCALVALSCATAPKGPDYGARMLPGTWIEDIAVESV
                     FEGASMTVPVRVYLPKGYDAKKPVRTLIGLHAHGGTNRDWSRHPAIVQFADKYDIALV
                     FPAMGTTLYESRYYPETTNKWNPMPGGRWMGEVLVPFLRARLGLARERIRTGIFGNAT
                     GARGAVLMACRYPAVFGATAGMSGDYDAISMIEDRLFTSVYGDMNKFRQRWENDDNVI
                     KLAANLKGTPVFLTHGEKDRTVPYEQSNILAIRILQLKKELGDYRVEYYLRYYRFHDW
                     GLWAGMIPAMMGFFDKTLAK"
     gene            27189..27725
                     /locus_tag="PKJ16_00655"
     CDS             27189..27725
                     /locus_tag="PKJ16_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45520.1"
                     /translation="MTPRRAIASGAAVFAIVAACAGCASVPAVTAYDYVCALHGTHHL
                     RDRAHTAACFGFPLAEYDNIRFFSREGAAQTDAQYDPLRARLTDEVENAIVDAVMQKI
                     QSAMGASIKRHWRFEQIKVPVVLRAFRHDSPGAIRATVVAAMHKDRLAPAAIIKYLPL
                     EYKMDIIDYEERFKDTAP"
     gene            27722..28489
                     /locus_tag="PKJ16_00660"
     CDS             27722..28489
                     /locus_tag="PKJ16_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV45521.1"
                     /translation="MITAGIDIGSITAKAAVMKDRQILGATVIFTGYNAELAGRRVFD
                     ELLGELGMTAPEVDRVVATGYGRASVKVATKAITEITCHAAGAHYLDPTVRSIIDVGG
                     QDSKAIVIDEKGGVRDFAMNDKCAAGTGRFLEVMARALEVDLDALGELSRAGERPASI
                     SSTCTVFAESEVVSLIAKGESRENIIAGIHDSIASRVVAMGNRIGVRGPAMMTGGVAK
                     NSGVVRALESRLGFPLAVSDYAQVNGAIGAAFLAFSL"
     gene            complement(28455..28967)
                     /locus_tag="PKJ16_00665"
     CDS             complement(28455..28967)
                     /locus_tag="PKJ16_00665"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNV45522.1"
                     /translation="MPILIDGFNLIYKFPELEGLMYEGKLAEARAGLLDRLKEYARLT
                     GAKVRVVFDGKKQPSLLIKSESAGVIDVYYSLDFSADHLIKEFIKKDINPRMATVVTS
                     DKEIIAYVTRFKAKVKTSEEFAKMVDEIIEASRAAAIPEKEDDPAVSQEDVSYWERLF
                     KGKKRETPRR"
     gene            complement(29185..30378)
                     /locus_tag="PKJ16_00670"
     CDS             complement(29185..30378)
                     /locus_tag="PKJ16_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013524507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV45523.1"
                     /translation="MKLSIVNTNDPESLVSAVGAAIPPSGIREFFDIVYSTPDCISLG
                     VGEPDFITPWRVCETGIFAIRDGCTHYTSNRGLLELRELIAARIEKETGVGYDPETEI
                     IVTMGVSQGLDIALRSIVNPGDEVIMFEPCYVSYMANVQLLHGVPVVLPTRLEDGFAI
                     DAARVEQAITPKTKAVLLNYPRNPTGSTIDRPTLERIARIAAERNIIVLADEIYSAID
                     YLDERFSINSVRGMRERTIYLNGFSKSHAMTGWRLGYVAGPKYLIDVILKIHQYTALC
                     APSIAQYAAIEGLKKGDGDVKRMRAEYLKRRNFIVSRFREIGLPCHNPEGAFYVFPSV
                     KPAGLSSRDFALGLLKAEKVAVVPGEAFGPSGAGHIRCAYATSMENLTEAMKRIERYC
                     TGLMK"
     gene            complement(30375..30644)
                     /locus_tag="PKJ16_00675"
     CDS             complement(30375..30644)
                     /locus_tag="PKJ16_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45524.1"
                     /translation="MQPFLDSAVKLIQGNQYVFLGVCIALVFLAGFILKQVKALAVVL
                     VLLASAMLYFIVYKGSVDTKKISEMKKQVKGKLIEAPKDVKKKRL"
     gene            complement(30648..31292)
                     /gene="fsa"
                     /locus_tag="PKJ16_00680"
     CDS             complement(30648..31292)
                     /gene="fsa"
                     /locus_tag="PKJ16_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HNV45525.1"
                     /translation="MKFFIDTADIAEIRKAKALGILDGVTTNPSLVAKTGRKYVEVLT
                     EIAKEVDGPVSAEVIATECDGIVKEGRELSKIGPNINIKVPLIEEGLKAVKILSDEGL
                     KTNVTLCFNSVQALMAAKAGATFISPFVGRLDDISADGMEVVEEIVAIYDNYGYDTEV
                     IVASIRNPLHIKHAALIGAHIATIPFSVFQGIIKHPLTDNGLAKFLSDYQKIPK"
     gene            complement(31402..32697)
                     /locus_tag="PKJ16_00685"
     CDS             complement(31402..32697)
                     /locus_tag="PKJ16_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV45526.1"
                     /translation="MVKYTSYALCIMIILTVFCSSNRPDPAPAARVGDAVISRGFLAH
                     VLSKMAADRTKQSERETLDRIVANVLVARKAEADGLAAAPGFAALAAAARTAALRDFL
                     CEQHGITDDRDAKLYALVVKTFELSAPAVRWDAIIREERRAPVPLGGAIPRRSRTTDA
                     NRDAALDRDAARAVVIVSRVVRSTLLDVLVGLDSDTLDGIIAGSPEERDMRLRAALTD
                     LHLPHLAERLDGPARALLDEVLVRARENLLVETYREHIGFEKLSGGRASSITYPVSPA
                     EAREFYDKNRAQFEEPQRIDVSHIRVRDYAEAERLHKAIVVDPSSFCALAAKHSIAPD
                     ARDCGRIGVIERNAAVKLPLYKEFGFTLQKPGQLSVPFVTPDGVEIVRLNSIEKRLRP
                     FGDPYTQKLIVEQMQPLKREAKLKASIEEMKKKYPVTMY"
     gene            complement(32902..33993)
                     /locus_tag="PKJ16_00690"
     CDS             complement(32902..33993)
                     /locus_tag="PKJ16_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45527.1"
                     /translation="MRKLFISITILLFCVALSGTGSAWKFNSHRDTVVKAFEFMESSH
                     ADYIQQWTANFLKYAGGSDIALKMGNKNGDTDNFYDTSIGGWWVGYRTHVGIFGFDTN
                     FTSFWHFITMFRPGNYGNAYSGYSYKYSLEDGFWGLNGVIKSLLYNQDVRNSGNAGNN
                     ISYPTNGKGVKDAYKFRYQLSAGQKYYSTTSGGNYDDYQDIIFEPNSNASAYWYAKAL
                     EGRTPSNIGAAHLYYLGHVFHMVGDSNVTQHVWNTSDHNHVDYEGWVDDNRASLVNFA
                     RVKELVQEFKNAHGITSPDKLRNVTLQQITVFFATKAVNRPAPLYSKDYSVRVTNGAE
                     EYNGSIAINVIVLEKYIYDLYLAESARKF"
     gene            complement(34043..34426)
                     /locus_tag="PKJ16_00695"
     CDS             complement(34043..34426)
                     /locus_tag="PKJ16_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45528.1"
                     /translation="MRILETLMNARPEADRAALTKHDGAPAGNTPAVTVDVDDVSRRA
                     SFIFRPYENAEEKRDVLDRLEETGALFDRMIDTTVDSIEKALSDGSRTTAEIDEAKAA
                     LAEMKKGRQFISGRIKMVENDEFSQ"
     gene            34865..35467
                     /locus_tag="PKJ16_00700"
     CDS             34865..35467
                     /locus_tag="PKJ16_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45529.1"
                     /translation="MAESDPRTELLERFNERRAKWQRQPSAPKRRFAPWLLLLNLIIL
                     GLMVSYHYRSAPDRTIHHTLVRYAGTAYRLTITHDESAKTIDVTLTMKNERAIPQRLF
                     FLDPVAVATFRHDEATVLSVPLGAGTRDLSIKPNDSRVFTVRLGEAHLRSFAEANPDA
                     IVPRRRTLFSAKRHIPLAVELSLRTRDALAAYLDFKYVIE"
     gene            35601..36305
                     /locus_tag="PKJ16_00705"
     CDS             35601..36305
                     /locus_tag="PKJ16_00705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45530.1"
                     /translation="MFRILPIVIVTTLAVSCGDMKCTSSLRERKTDDQIVKELKDKIA
                     RELKKDESSRQHQRLLDWYEQLGDRYLMMGVWEQAIVNLEKVIAQGRQSANLHYSLGL
                     AYANRGRDLNRPEDFDRAESQYRRSLDYGMVRNEAGFALAVLLYYQKNKKDDGLRLME
                     EFASKNKKHYRSRFGLARMYYDSGRPDQSLATYEELYGDLEKLPDSNVKNEYIEACKN
                     NMQRLQGELARPGSNK"
     gene            36421..37197
                     /locus_tag="PKJ16_00710"
     CDS             36421..37197
                     /locus_tag="PKJ16_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HNV45531.1"
                     /translation="MSLTYILGVTFGMVTLVFGIFSAGGNLLMFADLPSVFITVGGSI
                     ASLMVGYPLVRLLKIKDLFRICLFDSKFNYSELILTLVSFSEKSRREGLLALEDDLQD
                     LNDPFMKMGIQLVVDGNDPELVRKILEIEIDQMAQRHDSYKRMFDDWATLAPSFGMIG
                     TLMGLVMMLVNLQDKSTIGPYLAVALLTTLYGAVMCYVLFTPMATKLDMKTQEEVIVK
                     MITLEGILSIQSGENPRIVKDKLVMYLPPSEREAISREVE"
     gene            37201..37989
                     /locus_tag="PKJ16_00715"
     CDS             37201..37989
                     /locus_tag="PKJ16_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV45532.1"
                     /translation="MGKKFEKSKQKGAPPWMVSMGDMNNLLMCFFIVLMGEDVVKTQA
                     EDIQLIMSAIKGNVGIMDGGKTFARGKLAEMGHNLLSLPSTTRERTFAKGVKRAQELF
                     RPEIRAKQVRVTEDERGLVISLSSDIYFDSGSSRLKPEAKPVLKKLSGLFTGITNFIR
                     VEGHTDSRPIGTPGVREGFESNWDLSSARSISVVRYFIEDERVNPKQLSAVAFGQYRP
                     IDDNNTPEGRALNRRVDIVILHDRMLQEGKDPRISRPLPDEEWR"
     gene            complement(38003..39022)
                     /locus_tag="PKJ16_00720"
     CDS             complement(38003..39022)
                     /locus_tag="PKJ16_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45533.1"
                     /translation="MQTIFGFEWPVASGFDIALMIGLAALVVAFASLLRYYQYLKDKE
                     RHEEQFFNFKTKQLGLTNFQYKILLGIVRIVGLKDYRAVLHTPSLFESSIASFMSYIH
                     KKDESVDSLLAIGKDIVITYEKLYHPAAFRKPLDSIRDLETDSLMYFQTEDDRVFVGK
                     LTAKDQSSLALTLYRELKEGELLQEKVSVFVWRNGDAEYTFEATVSESDGNGVRILIP
                     DVFKRGREVRHPYLEVMIPCTLSWPVDREKGGGAEETDSVRGTICKMNEHEIVVRVGS
                     PIAFEREYSIDFTLSDFKIRSPLKTMADAVIHEENMHYYTFKFLEISEIAADIIRKYI
                     VERMD"
     gene            complement(39201..40154)
                     /gene="glpX"
                     /locus_tag="PKJ16_00725"
     CDS             complement(39201..40154)
                     /gene="glpX"
                     /locus_tag="PKJ16_00725"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HNV45534.1"
                     /translation="MDRNLALELVRVTEAAALYASRYMGRGDEMLANSSAIEAMAKVF
                     SSVSINGKVINGEQDPADGPLVKGQAVGTMNGIEADIVVDALDGKTTCAQGGHNAIAA
                     IAIGERGAFFNAPQLYMEKIAVGPEAKGVVDITQPPSVNIKRVARASGKYIEDVTVCV
                     LDRERHVDLISKIRETGARIKLIRDGDISGAVATAMEDKSIDILMGVGGAKEGILAAA
                     ALKCLNGDMQARFVCRCDEDRARIRDSGIDDPDRIYGIVDLVPGKNIMFSASGVTDGE
                     LLPGVWFRPGGAETHSIVMRSKTHTVRFVNTRHHFDYKPLY"
     gene            complement(40276..40602)
                     /locus_tag="PKJ16_00730"
     CDS             complement(40276..40602)
                     /locus_tag="PKJ16_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45535.1"
                     /translation="MGYWAEVREIALKGLDLAAQNIKEGAAIAVEKGKEGMAYVQLKK
                     DLILAQRKLHNLLADLGDVVCDLYKEKKDVYADAVVKETMDKILAAENECKKIEAEIK
                     VIGAKQ"
     gene            complement(40798..43299)
                     /gene="hrpB"
                     /locus_tag="PKJ16_00735"
     CDS             complement(40798..43299)
                     /gene="hrpB"
                     /locus_tag="PKJ16_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HNV45536.1"
                     /translation="MSPDDIAALPIYPHLDEIAETLARHSAMLLHAEPGAGKTTLVPW
                     RLLREKECAGGKIALLQPRRIAARAAAERIAALLGEEIGSAVGLRTRTETISGPAARL
                     EVMTEGVLVRIIQGDQSLGGYGTIIFDEFHERSLVGDLGIALAIDCKKHLRSDLRFLF
                     MSATMNADALRDTLGDVPHRSIPGRAHPVEIRYSPPRGAEAPWDAAGRLALEALREIQ
                     CAGDVLVFLPGFREIRRAREACAGLSSADAEIMVLHGRVRPEEQRAVLRRPETGRRRI
                     ILATNVAETSLTIPGIQAVVDLGLERRVRFSPRTGMGHWETVPISAASAEQRAGRAGR
                     LGPGLCLRWWDSRESRSDFAPPEIAEADLAPLVLECAHWGAPSPSDLAWITPPPEPAV
                     RRAQSLLSDLGFLDGRGRITDAGRRAARVGAHPRFARMLFDGIDRGAAATAAIIAAII
                     EEGDPLPGADVDLRTRLDACREWLTGSRGRLSGSGAGRIADAATRLLSQAGVRCSAID
                     AEAIDPNLAGLLLLGAYPDRAARKTRDDGPARARLVLAGGRGATVRGALAAEEFLAIA
                     EIDGGESDGRVMLAAPIARADLEGGAAGPVRACCTFEWRGWTPAVHVERRVGALVLSR
                     QSGGSPAAEELQRAVRERIMREGIQSLPWGETSRRLCARCRFVHGRGVRNDWPDFSDD
                     ALSREVDSWLVPHGTWSGGPVFDERSIVRALEGRLGWENRRLLDDIAPEIWTLPSGSR
                     RPIDYTAGDIPVLAARLQEFFGSVETPRLCGEPIILHLLSPAGRPVQITRDLDGFWDR
                     AYPEVKKELRGRYPRHFWPDDPRAAAPTARAKPRK"
     gene            complement(43356..44408)
                     /locus_tag="PKJ16_00740"
     CDS             complement(43356..44408)
                     /locus_tag="PKJ16_00740"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HNV45537.1"
                     /translation="MIERIRKALGSDNESLLTYAARVPKERITLPGPDFVDRVFAPSD
                     RPVPVLRSLQALFAAGRLANTGYLSILPVDQGIEHTAGASFAPNPEYFDPENIVRLAL
                     EGGCNGVASTLGVLAMVARKYAHRIPFILKINHNELLTYPTKYDQVLFASVEQAVDMG
                     AVAVGATIYFGSEESSRQIQEISAAFERAHELGLATILWCYTRNNAFKTKEADYHTAA
                     DITGQANHLGATIKADIVKQKLPTLNGGFTAINFAKTHDKVYTELSSEHPIDLTRYQV
                     ISNYMGRIGLINSGGESSGERDLAEAVRTAVINKRAGGMGLISGRKAFQRPMKDGIAL
                     LNAIQDVYLCAEVTVA"
     gene            complement(44445..45191)
                     /locus_tag="PKJ16_00745"
     CDS             complement(44445..45191)
                     /locus_tag="PKJ16_00745"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV45538.1"
                     /translation="MMRRFLMVAVCACGVPLALYANDDGRDLVRKSMALAQPQTAKFR
                     YKMLVFRGEKVLEQHFDQYVKRNPDRENKMRITFTHPARLELLSHAHKNRDDDQWLKM
                     SAGNVKRIVGTDLDKPFANSNLYFEDLKELNVDDYDFMHRGTVKAVGEECHKIEAIKK
                     RGTVVYSKLVLYLRTYDQYIVRIDYYLKDEFHKYLERINVKPVGNYLTPYRIIMYRAD
                     GRGKTELKLAGNPEYDVPIADAFFITEKFR"
     gene            45365..46144
                     /locus_tag="PKJ16_00750"
     CDS             45365..46144
                     /locus_tag="PKJ16_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45539.1"
                     /translation="MRNALCAICIALVSATCAGRDASMRRETTAIRPDSLVNFFRVCE
                     QDSKNAIAVIVNRDITKNLTHLQRMDGGGRKYYLLEREAITEMIRAVTSGLYDDFILI
                     NRHGVVVYTMADDALFAKSVPASFKGTALQECFEKTGTDLYLSDTGPSPGASERPVLY
                     AAGRVVRDGEFHGVFLLQVRAERIARLIAENESIVSRAGIVRISRNGIPADAPYPDFE
                     RIAPATLVEARSHRLTLPRAVLSYYPFTYKNLSWIIIARER"
     gene            complement(46125..47399)
                     /locus_tag="PKJ16_00755"
     CDS             complement(46125..47399)
                     /locus_tag="PKJ16_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45540.1"
                     /translation="MKLLSEISVRIKRGFLPHLTNELYKRGCEMRSLAQLEQDGGGDL
                     FAIEVQCDSMEGFAAFLERIGRFSENFELVSVRNALDELIEGGLLRVSGGLPLEQADD
                     FETKVLGATALLLERAASGGSARTGIARNVAVVSGVRANPGGGTAPVAEEYVRNERDA
                     LVVSRFAGLNAYPLVIVFDHIEDFVKSLRAIEGTFAAVRISSVGEVEDPSDLEQVSAG
                     VTVPLVTRDHDETPALLLAVIDGLSEKNGLERGETSVGFAGIDCAAIRLTHLLAARGY
                     RRILGCDASETLMLALEKAGGLATTLENIFGNADIVVRGARDVTEADLRKIRPGQLVI
                     SLVPVTAEEEAVFRERGVRAHVVVSEQHRASVLPGLLMGAHRAGLCGIDDGRIIAASR
                     LLVESASPGAYLPELFGGIHDRLAGLISAPAR"
     gene            complement(47465..48040)
                     /locus_tag="PKJ16_00760"
     CDS             complement(47465..48040)
                     /locus_tag="PKJ16_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNV45541.1"
                     /translation="MALVLSLFGSPRDTSVSSHLHERFLAPLAAAGHEVRRYAAYAMD
                     VRPCIACGHCRDSFSCVHDDEMTALYPLLVQARLVSVSSPVYFSGLPGPLKTVIDRCQ
                     ALWELARRECISGARDGFFISVGEGDYPRQFEPSVVTMRHFFNSIGCRPLESDYLLVR
                     ADPDASPEHIAEAARAETAGIRSATAMSGRA"
     gene            complement(48045..49049)
                     /locus_tag="PKJ16_00765"
     CDS             complement(48045..49049)
                     /locus_tag="PKJ16_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HNV45542.1"
                     /translation="MINVLRGSFIALSLICSVLYYLEVSWLAMLVAAVVSVVVSLLVI
                     IAIEYVSHTFSARLLFAAIIGLASGLVVGHLLALAFSALPLPFSAQTLAVVRALLYHV
                     SGFAVLLFFVINHEDIRFLDAIVPARAEDDRGGTIAYKILDTSVIIDGRIADICDTGF
                     IEGILVIPNFVLGELQMIADSADSIKRNRGRRGLDILNKMQKDQSIMVKISDMDFPET
                     HEVDSKLVKLAKAMKAKIITNDFNLNKVAEFHGVDVLNINQLSNALKPIVLPGEEMRV
                     SLIKEGKDPNQAIGYLDDGTMVVVENGRSRLNSEVHVNVTSVLQTTAGRMIFARLRED
                     "
     gene            complement(49225..49707)
                     /locus_tag="PKJ16_00770"
     CDS             complement(49225..49707)
                     /locus_tag="PKJ16_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HNV45543.1"
                     /translation="MYQLGDKVVYPMHGVGRIDSIEKKVVLGKKTEFYIINIINSGMK
                     VMIPVDNAARIGLRPIIQKKDIKKVLTIIKQENVVTEDDWKLRYQNNIDKVKSGSIFE
                     VAQVTRDLYRRGRSKELSIMERKLYENAYQLLIHEIALTKDVAVEEAGNLISEALSSV
                     "
     gene            complement(49862..>50843)
                     /locus_tag="PKJ16_00775"
     CDS             complement(49862..>50843)
                     /locus_tag="PKJ16_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV45544.1"
                     /translation="TFTVDNQFNRAMQARRQPGSSFKPFVYGAAIEGEYVSDRSVLPD
                     APIVEIDSEGVGWSPTNYEGDFKGLVDVRRAVAASINIIAVRVFDLVGPDNVIDFAAK
                     MLKVPPSRFDPNPTLALGTTEVTPFEMATAYAIFANKGREVIPFAIRYVIDKDGNEVM
                     NVEEEVGNIIAAKERDGTVQVISEQTAWLVNDLMRGVIDGGTGNEPIRYKAGFLKQAA
                     GKTGTTTNWSDSWFCGLTPDIAAIVWVGYDKATMTLGAGAAGAMVAAPIWGRYMKDVY
                     NGMPDPAFPKEPSGVFHGAIGPYSDFKGSKMKSVLERYMEREGISLDENQ"
BASE COUNT         9040 a        16927 c        15299 g         9577 t
ORIGIN      
        1 ccgttcgaaa atgtcggcgg ccttctcctc ccagcgggcg cgctcgtagt cgcggtgctc
       61 gcgcttcttc tgccactcgt agttctgcag tgcctggcgc tgcataagct cggcgtcgtt
      121 gatccccgtc cgggcgccca ccagcgcggc gaagcgctcc gcgagacccg gcgcgccctc
      181 gtctatcagc atgtcgagct cccgcatgtc gtcaaaggag aagccctcga gcacaccgta
      241 atagtccgtc atcgccttgc ggtaccccgt gtaggcgcgc cccgcctcaa gtgcgctata
      301 atacccgttc gcctgcgcat cggtcgcgat ctcgtaaaag aggttttctt tggcacgcca
      361 aacaacaacg gaatcatact tcgggctccc ctctcccccc gggagaggct cccaacagga
      421 cgttgcagtg ccgagcgcgg acaggatgtc tgaaaagcgc tcggcggccg ggggtgaggt
      481 catcttcgcc gtataatcaa aatattcctt catatcatgc aaccgccgct gattcatatg
      541 cgcggtgaat ttcgacgtca ccggggcgga gttgtaatac cacacccccg tcttgtcgca
      601 ggtatcacac acctcgccgt tctcatcgaa caccacgctc gggttctccc gcgccggcgc
      661 cgcatcgaag atgttcgcgt cgccctgcgc gccctccata atgtacgcca ggtcctcgtc
      721 gcttatttca aagccgttgg tgccacccag ctcctttatg accgtcttca ggctccgctt
      781 gcgtacggtg atccatttcc cccggggatt gcgataggtg atcacgcccg cgttcgcgtc
      841 gtcgtccagc gtctcgatct gcgtgatctc cgccagcttc accatcgcct tctgcatgcg
      901 gtccttgcgg ttcctgagtt ccgtgagctc ggcgttaatg ttgttcacgg cggattgcgc
      961 gttcgagaga tgattgttcg cggcgcgata atgcgaaccc atgtccatgg tgataaagtt
     1021 gacgagcgaa ctcaccacct gattcagcgt gccgcgcaac gcgtttaaca actgcttgag
     1081 cgggtccgcc atccagcgca tgcggcgggg gatgcggttt atctggtttt gcacggcgtt
     1141 gatggcagac tcgagcgcag tttttgcggc ccaattcgcc aacaacaaat gcgcgccagc
     1201 accccctacg ataagaaacc gtgtccattg cgctgcctcc tctttgtgcc ggagcatctc
     1261 cgtggcaaag gtggtcatcg cgccggtcaa ttcgccaccg gaaaacacat tatcccaact
     1321 gttctccata aaaccgtaaa aatcgctccc cgacggctca gagccaatcc agtacggcgg
     1381 tgtgcggaag atcgcgtcct tgttggagtt gtaatagtac gccatatcca cgaactcatt
     1441 gtcgtgcgtc atggcgggta gcacccatga gagccaatca atatgaccca gaccgggtat
     1501 ctgcgtgacc gcgggacgcc aggtggcaac atacatgtcg cgcgtcatga aataatccag
     1561 gaactcgttt cgcttcttct cgttctcgtc gccgttggcg gcgatcagcg tgtcgcggta
     1621 gctttcgtac gctgcgcgcg cctgctcgta ctcgaatgtc cgcttctgca cctctttatc
     1681 aatcaaatag ctcgccttgg ccaggcgaaa ataacgctgc gacttctcct taagctcctc
     1741 catcctcgcg acgtactcgg cgtcggtgag cacgtcctgt tcctttaccc ggttcacctc
     1801 ctcctgctgt ctggccatgc gctccagcac ctcattgtgg atgccttgca ccatgtccag
     1861 ctcctgcttg gtgtcctgca tcagcttctc gggaccacgt ttatcgtcgg cgttggtcga
     1921 ggtgttgtat agatacgcgg tcgcggcgta ctcgtacacc gccttttccg tatcgtagac
     1981 gtcaatggcc gcctgtagcc gccggtagat ctcggcaatc gcattcaact ggctaaagta
     2041 gtttccctgc gcggtgcggt agatggcctc gcgcgccgca cgctccgcct gcgccatatc
     2101 gtatgcggct tgtttttcct gatggacccg ctttgctctc acgtagtcaa agccgttttc
     2161 tggatcgtca gtcgtcacat acttgctcgc tgtcatctcc aaccccttct ggatcttcgc
     2221 cacgcgctca atctttttct tcgccgattc gtactttgtc acgtcctcga gatactcatc
     2281 gcgcgccttc gccacaatat cggagtcgcc ggtcgcgatc caggccgtat gcgcgttctc
     2341 gatctgcgcc gtcagcgtgt ctatcgccgc gagcttctcc gcgcgcaccg catcgtccat
     2401 gtccgccccg taggggttca tcgtatcggg gcaatagttc gcgcccatga ctgcgagcag
     2461 gtcctccacg ggcgacaggc catgaagatt gtcgaaaccc gcaagggagg ccgcggcgag
     2521 cgcgtcttcc tcgatccccg acaccgcgta catcacatac agcgccctat tatactccgt
     2581 catcgcgtca atgaggctgt tcgcgagacc atcctcgccc gcgcggtgaa agaaccgttc
     2641 acccatcttg tccgacagta aaagtcccac gagctcctgg tattcaatcg gatagcgcca
     2701 cttcacctcg atgtttttgt cgggatcccc caccgtagcg ttcaaatagg cgtcgcgcac
     2761 ctcgtcgggg ataagatact ccacgtccga tatcttggta atcaccggct tctcggcgtc
     2821 ctccgctgag gggacctcgc cgggtttcag caccacaaag cgatcgtcgt tgccgtccgc
     2881 cacgaatttc ccaatgtagt agtcccagtt ctggaaggcc gtgtcccacg agctccggaa
     2941 attgtcaaag gcgtccggcc cgaggcgggt gtagtcgtag agcgacgtcg ccttcgcgcg
     3001 caacagcccc atgtcctcgg cgtaatccgc cgtcgtccgg tactcgccgc ccttcatgtc
     3061 cgccccgtac agcgtggtga tgagcgccgc aaaatcgcgc gtcgccgcgt cagaggccat
     3121 cttgagatca aagcggttcg tgagctccat gatggcgtac ggggaggccc cgtcggggga
     3181 aaacgcgtat cgccgcaaaa aatccctgtc actatcgccg atgatgcccc gcctctcaat
     3241 gaactcctcg atatccgcac cgtactcgta ccccgacgcg atcgacagcc gttcgcggta
     3301 tgcgcccagg tcgtcccgga acgatccgta cagcggatcg gactcggcca cgcccatccc
     3361 cacgaggaaa tcgtccaccg tcatcagcag gcccttcgat gcgaagaaat cggcgaagta
     3421 ctcgccccgc agcaccgtcg cgaccacgtc cccggaaagc tcgcgctcaa tgaacacgtc
     3481 ccttccagca atcgccgcgt cgtagatctc ctcgaacgag ccgcgccgcg catcgagacg
     3541 atccgcctgc gcggcatact ttccgacaag cgcggcgaag agatcctcga acggcgtatc
     3601 gtcgatcgca tcgccccgcg cagcgcatat cgccatgaac tcgagaaggt agccgtaatc
     3661 gcggaagtcc gacgccagcc ggtccgccca gaagcccccg ccgcccaccg acgcgttcag
     3721 ccgatcgtag taggtcagcc gagcgaacag cgcgaggtcc gccgcctcat cgtcggcgag
     3781 cgacgccgga tcgacgcccg cggccgcgag aaaaccgttc agccagtagc ccaccgtgtc
     3841 cgccatctcg ggaagcgcgc ccgcaccgag cgcttcgcgc accttcaagc gcaggtcgag
     3901 cgtccgcaag cgcgcggcca cctcgtccgc aaagcccgcg cggtcgtcgg tgaattcgtc
     3961 aatgcgcgca aaggccgcgt ccccttctgt cagcagcagt cgctcgcgga acgccgccac
     4021 gctgttcccg tatgcgaagg aatgcgcaag gtattcgtac atatagaact gcgtctcgta
     4081 ctcgaagcgc ttcgccctaa gcgaggcgat aagcggcggg atggcgctta taatgttcgc
     4141 gctcccctcg acgcgcatcg tgaagttcgc gagctgcgcc agatcgtcga tccgcgcctc
     4201 gagcgcgcgg acggtttcat gtatcttctt cgtatagagc gcgtaccggt tgcgcgagta
     4261 aaacgctcgc tcctcttcgc catagtcctt cgtctgctcg gcgagcgcct ccccgtaggc
     4321 cgccatcacc tgatccaggg tctccacctg ggagacgatc gtatccagcc gcgccttcgc
     4381 ttccccctcc gagagcgcgc ggttttgcaa tacatagtct cgtttcagca tgtcgatgta
     4441 tttcgattcg gcggccgcga gcgcctcgcg caccgcgacg ggcagacgct gatactcgtc
     4501 gctctttcga taggcccgga gcgcctcccc gacctccgcg agcgcctcat agctctgtcc
     4561 cccgatgaag atattcttca cctcttcaac cgtcttcccg atcgccgcgg cgatgcggga
     4621 gagcactccc gaacggtcct tcacgcgccg ctccaccgag gcgtcgtagt tgagcgccgc
     4681 gtacccggcg agggccgcct tcaccgcata gtcagcgccc tcggcggcca cgagccgcgg
     4741 atcggaaaac aggagcgccc cctgcccgct cgtgagggcg ttcacgtacg cgccgcgccg
     4801 ctcgatatcg gtatcggaaa gaacgtttcc gtcggcgtcc ttcccggtag cgagctcgcg
     4861 ctgccaggcg gtcacccgct ccatcacctg ctgcggcgtc gcgtcgggca tgatctccgt
     4921 cgcgtagaga tctctgatgt atcccgcccg acgctcctcg aaggaaatcg tctccatgaa
     4981 cgcgtcccac gcatcgttgc gctcggccgc gttcgtcatc gccgcgatgt cgtccagcgt
     5041 ctttctcgcc gtttgcacaa taaaacggta gatgcccgcc aagcgcgcgc tttccggacc
     5101 gacccctcct ggatcgtccg caaagcgttc gcgcgctcgc acaagaagac ctgcggcaag
     5161 ctcattcgcg ctcattccct tcttccccgg ttccttcaga taggcggcca aggcggttac
     5221 ggtctcatgg ttacgctccg cctcgtatcg attgaagcgc gacgcgtcct tcgcgcgcgc
     5281 ggcaagccgt gccgcgctgt tttcggtgtc gtccgtgatc gtcgcaacgc cgttgaaatc
     5341 gtacaacagg taggtcacca cgcccttggc cgtgagaaac gcagtctccg ccgcgacgcg
     5401 cgcatcgccg atcgcgccca tgagctcgat ctcgcgcgcg cctttctcca gggccgccgc
     5461 atcgccaagg agcgcatcca gcgccgggcc gctcaggccc gctatctcaa caaggggttt
     5521 tcccgtgatc gccatcgccc aggcccggag catcatcgat ttgatctcgt ccggcgttgt
     5581 accgctaaaa tccgtcaggg cgtacgcgcc gtacgaatac cccgcgattt ccgcgagcgc
     5641 ctgatttttc tccgcgtcgc tcccggcggc gagagtcgtg aacgcgttca tgacgtccgc
     5701 gagcgttttc tccccgaaca cgcccgaccc cgcgcgcccg ggcagcagtt caaacatgat
     5761 cgcctcgcgc tccgcgtcgg gatcgtcgtc aaaccgcgcc acaagcgccg taaacgctgc
     5821 gtgccggtcg tacgccgtct cgagggcggc tcgcgcgcgc gcaatccctt cgttcgccgc
     5881 gtccatgtag tacggatcgt tcttgtcaat cgagatgaaa atatcgtcgg cgttgtccct
     5941 caccgcctgc gccagcgcct caaacgagtc cccgttcgcg cgcgcgctcg cgacgatctc
     6001 cgcgaaccgg tcgctcccgt cgcgataccc cgcgctatcg ccggccccct cgagcacgcc
     6061 gatcgcgtcg cgcaggcgct cggcgtaggc cttcgcctcg cgcgcctccg cgcgcagctt
     6121 gaccgccgtg tagtacgccc gcgcctcgcc gatgagccga tcatccgcgc ccgcgacgct
     6181 gcgcctgagg tcgctcaccg tctcagtgag atcgtttatc tcgcgcagca ggatctcccg
     6241 cgcattgggg ttttccagat acaggagctt ctcgtttgcg attcggaact gttcctcggc
     6301 cacatcgtac tccgcctgcg cctcctccag cgcctcttgc gcgtcgtggt agttcgtttt
     6361 cgcgctctcc acggcagcca gaaactcact cgtgaggcgc tcccgcgcgc ggtcaaactc
     6421 cgcctgccag tgatcgcgtt caaccctcgc ctcatccagc gccgccgcct ggtcctcctt
     6481 cgaggcgcgt cccgctttgt cgtaggcgaa atcgtacacc gcctgcgcca tcagcaaccg
     6541 cttgttccag aacttttcct ctatcttgat cttctcgagt tcaaactcgg ctgcggtcat
     6601 gagatagggg tcgcgcacgt tttcgatgtc gcccgcgaag accacgcggc cgtcgtcggt
     6661 gtactcgaga tagaagcggt cgctccgtcg gtcgtccaag agtccgctga agacgaagcc
     6721 cccgatcgtc ccgccccaga tgtccccggc gtgcagcatc atctccgagt tcacgagtat
     6781 ccgctcgaag ttctccatga tctcccgcca gcgcgcgatc tcctccgaca ggagccccgt
     6841 catggagccg tcgcccttgc gggcatagcg cgccgcgagg tcggtcatcc aggcgatgct
     6901 ctcctggccg ttgagaagcg cggcggaccc ggtgtccgcc attcccctga gcgtggtgat
     6961 gtaatcgtcc cacgacgcgc cctgcgcctc caggtagcgc gcgagctcgt cctgcgcgtc
     7021 cagatagttc cggtcaaact ggtcaaaggc gttatcccaa acgagcccgc cctggtacag
     7081 ttgcgtttga atctgggtga gccattcgtt cttgcgcgcg cgaagctctt cagaggcgtc
     7141 ctcccaggcc ctgtcggatt tcgcgaagtc ctcgccggcg cgtttctcac acgccaggcg
     7201 cgccgcgaac aggccctcct cggcgtttcg ccatgacgcg agccccgcgt cgatgatcgc
     7261 cttcacgcga tccgagtagc cctccaccgt cgggacaccc gcatcgccct cgtaggtcgc
     7321 gctgccggtg tcgagcgcgc gctccatgag gtcgtcgatc ttcatccgcg tctgatccag
     7381 gatcgatcgc gtgataaccg aggccgagtt cgcctcgctc tcgcggcgaa gcgagtccgt
     7441 atcgtaccgg ttccggtaga tgaaccggtt cttcgcgccg ataagataga tctcctcgtc
     7501 cgccaatgca tccgcacgca tccgggccag acgctcgtcg agccccgcga cgaaccccgc
     7561 acgaagctcg tccagatact cgatccccgc gagcgcccgc tcccgctccg cctcgagggc
     7621 cgtctcgtag ttgccccaca gctccgcgaa ggtctcgccc gcctcggcgt tccacgccac
     7681 ataggcgtcc gtgagacccg tcccgaccgg cagcgcgtcg gcgcgcgcga tggaatcgag
     7741 caacgtctcg tggtcgaagc ggggcggcgc aatcggcgcg accgaggcga gccatcgtcc
     7801 gcgctcgtac tcgatgtccg cgcgcatcgc cgcgtccagc tccgcctcgg cgcgcgcgaa
     7861 aagctccgcg agctcgacgc gaaccgcctc cgcgtcctcc cccgtgccga tgcgcgcgac
     7921 gagctcacga tcaagcgccg cctccgcctc gcgctcccaa tcggccttga gcgcgtcgcg
     7981 accggcgttc accacgcgca cccattcggc ctcgtcccgc ttcaccgagg ccgattcgaa
     8041 ggagcggtcc agtttccgct ccacctcgcc gtagcgtttg aggcgaacgg tatcggcggc
     8101 gagcgggacg gcgacaagca ggcacagcat ggcgcgggaa aggattccgg gcagcattgg
     8161 gcaggacctc gatcgtgaac gccgcgaacc ggaatagcgg aactgcgcgg cggggggcga
     8221 tggaacggcg gtcgccatca accccctatt gtagatacgc gtgcggaaaa aattttttta
     8281 acttaatatg cgggtttcgt attacaggta tgacagcagg aataagcgta acacggccca
     8341 tcgcggtcgt catggtgtac ctcgccggca tggtcttcgg ggccatcgcg ctttcgaaga
     8401 tggaggtgga cctccttccg cgcgtggagc accccgagct ctcgatcatc acgctgtatt
     8461 cgggggcctc gccgcgcgag gtggagtcgc tggtcacgcg gccggtggag gaggccgcca
     8521 gcggcgtgcc cggcgtgcgg cgcgtgcgct ccgagtccat cgagggggcg agcctggtga
     8581 ccgccgcctt cgagtggggc gccgacatgg atttcgcgac catcaagatg cgcgagaagg
     8641 tggacctcgc gaagggggcg ctgccccagg acgcggagaa atcgatcatc gcgcgcctcg
     8701 atcccgccgc gcagccggtc gcggggatcg cgatccgggg gcgcgacggc ggcgtggacg
     8761 gcctgcgcca gatggtgcac gcgaagatca agccgctctt cgaacggatc gacggggtcg
     8821 cctgcgcgcg catcgcgggc gggcgcacgc gcgaggtgca ggtccgggtg gaccagtcgc
     8881 ggctctacgc gaacggcctg ggcctgcgcg acgtcatcga gagcgtgcgg ctgtccaact
     8941 acaacttccc cgcggggagc gtcacgcggg gtgcgcgcga gttcgtgatc cgcaccatcg
     9001 gcgagttcgc gagcctggcc gacatcgaga acgtcctggt gcgcaagtcc gcgcggggga
     9061 gcgccgtgta cgtgcgcgac gtcgccgagg tcgtggacgg ccaccgcgac cgcgacagca
     9121 tctcctatca caacggcgcg gagagcatcg ggctggccgt cgtcaaggag gcggggagca
     9181 ataccgtgcg cgtgtgcgcg gccgtgcgcg acctcgtctc cgagatcaac gcgcgccacc
     9241 ggggcgccat cgagctcgcc gtctgctacg acgggtcgcg ctatatcaaa aacgccgtgg
     9301 acaatgtggt cgatgcggcc gtcctcggcg ccgtgatcgc gtttttcgtg atcctattct
     9361 tcctgcgcga cgtgaagagc tcgctggtca tcatcgccgt cacgccgccg tcggtgctca
     9421 tcacggtgct cgcgatgtac ctgcggggga tcggcctcaa catgatgtcg ctcggggggc
     9481 tggcgatcgg cgtggggatg ctcgtcgact gcgccatcgt cgtgatcgac tccgtccacg
     9541 cgcgcctcgc cgccggggag gatctgcgcg cggcctgtcg gggggcgatc gcgcaggacc
     9601 gcgccgcgct cttcgtctcc acgctcacca cggtcgtggt cttctttccc gtcgtcttcg
     9661 tgcgcggcat cgcgggacag gtcttctccg acctcgcgtt cacgatatcg gtttccatcg
     9721 tgatctcgtt cattgtttca atcacactca cgccggcgct cctggtcctt ctggagcgcc
     9781 ggtcattgca atcggggggg atgatggggt tcgcgccgct ccgacgcctg gcgaatattc
     9841 ccgcgcttcg acgctcggcc gaacgtttgc gcgccgcgct cgaatgggcc cattcgcttc
     9901 ttgaacgatc gtataccgcc tatctcgtcc gtctcgagcg ccgcatcgac ggataccggc
     9961 gcgcgctcat catcggcgtc acgctcgtga tcggcggatt cctcatgttc gcattcctgg
    10021 acacgtcgct ttttccccgg accaacagcg gttccttctc gatccgcatc gtcatgcccc
    10081 cgggcacgcc gatagacgac acgcgcgagg ccgccctgcg cgtcgacgcg gcgctggccg
    10141 cattgccctt cgtggagaac cgtttcatcg acgtgggcta caatcccggc gagatctcgg
    10201 aatacttcgg ccgcgagaag agccgcgcga cggccgagct cgtggtcacg ctgaaacgcg
    10261 accgcggcgt gtccactgaa gcggcgatgc gcattgcgcg gcgcgccgtc gcgctcccgc
    10321 ccgagattgc ggtggagttc ttctccgagg agcgcgagtt cgcgggcttc atgcggctgg
    10381 gctcctcgac cgtgctggtg gacgtgacgg gcgacgattt cgacgcgatc gaacagtcgg
    10441 tcgccgtcct cacggccgcg tttgaaaaaa cgccgggcat cgcgcgggcc gtgccgcacg
    10501 tggcgaaggg caagcccgag gtgcgtctcg tcatcgaccg cgcgaagctc gccgccttcg
    10561 gcctcgcgct gggcgacgtg gccgggaccg tgcgcgccgc cgtccgcggg gaggatgccg
    10621 ggaaattcta cgaggccgac gatcaggcgg acatcagcgt gcgcctgcgc gcggccgacc
    10681 gcagcggcct ggccagcctg ggcggcatgc tggtgcgcgt gggcgaatcg gccaatgtcc
    10741 cgctcgccgc cttctccgcg accgagcggc agtcgggcta caacaagatc acccgccggg
    10801 accagcgccg tacgttctcc atcgcgctcg acgccgtgcc gggcacgggc cgcggggccg
    10861 ccatcgcggc ggccgcgggc gccatcgaac gggtgcgcct cccgcacggc gtgcacgccg
    10921 ccgtctcgcg cgacatggac gaggtgcgcg actccctcgc cgagctcgcc ttctcgctcg
    10981 cgctctcgat gctgctcatc ttcatgatcc tggccgcgca gttcgagtcg ctcacccgcc
    11041 ccctctacat catgctcatc atgccgctca cggccgcggg cgtttccctg ccgctgctgt
    11101 tcaccggaaa cagcgtgaac gtcatctccc tcatggggac cgtgctcctc tcgggctcgg
    11161 cggtcaatgc ggcgatcgtc ctcgtggact atatccaggc ccggcgcgcg gggggggcgg
    11221 acctgcgcgc ggccgtgatc ggcggctgcc gcgagcgcct gcggcccatc ctcatgagca
    11281 cgtgcaccaa tgcgctgggc atcgtgccgc tggccatcgg cctgcgcgag ggcggggaga
    11341 tgcagtcgcc catcgccatc gtcttcttcg ggggcatcgc cgtatcgacg ctctgcacgc
    11401 tggcgctttt gccggcgtat tactatcatt acgagtcgcg ggccgagcgc gccggcaaac
    11461 ggagcgccaa tggataggcg acgcgtgcgc atttcgcgtt atgcgtatca ctcaaagcga
    11521 ccgtgcgcgt ttgtcgctcg cgaacgaccg gcgctcgaac gcagggggcg ataggcgcat
    11581 gcgagaaatc gtccgcttct tcatcacaag aaaaatcacc acgctcatgc tcttcgcggc
    11641 ggtgctcgtc ttcgggtgcc tctcactcgc gcgcatcccc accgcgctcc tgcccgccgc
    11701 cgcgaagaac cgcgtcacca tcgcggtgcg ctatccgggg cagtcgccgc agcgcatcga
    11761 gcgaatcatc gtcatgccca tcgaggaggc ggtgagcgcg gttggcggca tcacctccat
    11821 ggtcgcggcc tgcaaagagg gcgaggcgcg catccacctg gccttcgccg agggcgcgga
    11881 catgaaatac acgacagtgc agctgcgcga gcggatcgac ccggtgcgcg cgagtttccc
    11941 gcgcgacgcc gacgagcccg agatctacgg cgcgagcgcc gacgagcgcc ccgtgctcat
    12001 cgtcacgctg cgcagcgaca cgctcgccat cgacgccctg cgcgagatcg ccgagaagcg
    12061 ctacaagaag ctcttcgagc gcatcgaggg cgtctccgag gtggcggtgg gcggcggggt
    12121 gaagcgcgag atcgagatcg cggtgcgcga ggaccggctc gtctcccacg ggatcggctt
    12181 cgaccgggtg atgcgggcgg tgcagatgaa caacgtggct gtgccggccg ggacgatcgg
    12241 cgagcgccgc gaacacgcgc tggtcatcga cggcaaattc cggacattgg acgaggtgcg
    12301 cgccacgccg attctcgcga ccgcggccgg ctcgccggtg ttcctgggcg atatcgcgtc
    12361 ggtgcgcgac tatgccgggc ggcgcgagga gctctcgcgg cgcaatggcg ccgagaacgt
    12421 gtcgctctat ctcatgcaga cgggcgcggc gaactgtctg gagctctcgc gcgccgtgcg
    12481 cgaggctctg cgtgctgcca atgacccgcg catccacatc gacatcgcct acgaccaggc
    12541 ggagttcatc ggccgggcca tccgcaacct cgcgataagc tgcgtcacgg ggatcgcgct
    12601 ggcctcgctg gtcctcttcg cctttttccg ctcggcgcgc gaggccctgc cggtcatgct
    12661 cgccattccg ttttcgctcc ttcccgtgtt cagctatctc tacttcagtg ggatgacgct
    12721 caacgtgctc tcgctcacgg gactggccct cgcggccggc atggcggtcg acagcggcat
    12781 cgtcgtcctg gacgccatca acaagcgtac gcaggaggcg cgcggcgcgg cgcgcaacgg
    12841 cctcctctcg ccggatgcga tgaatacaat ctgcctcgac gcgacggcgg acgtggcggg
    12901 ccccatcttc gcctcgaccg tgacctccgt gtgcgtcttt gtccccatgg cgctcttcgg
    12961 gggaccggcc ctggggctct ttcgcgacat ggcgggcgcc gtcatcgtgg cgctgtgctc
    13021 gtcatttttc tcggcgctca cgctcatccc gctcgccgcg gcgcttgcgc accggtttga
    13081 tgtcgcgctc atcatcaaca cactgccgtt cggcgcgctt cgccgcctgg cccggcgcgt
    13141 tgccaacgcg cgcccggtcg ccgcagttca tgcgatagcg cttcgcatcg tcgcgcgcac
    13201 catcgcatac cgcactgctc tcccgcagta cgaggtcctc atggaacgcc tgctgcgcaa
    13261 ccgcgtcaga gcggcgctct ctgttgcaat gatcctcgcg gcattattgt tcctgtcgtc
    13321 ctttctcagg gaggagcatc tcgacccgct ggagtcgaat gagctctcgg cgacagtgga
    13381 gctcgccgcg ggcaccagcc tggaggagac ggcgcgcgtg gtcggtaata tcgaacggac
    13441 gattcgcgcg gagcgcgccg tgcgcgagct ctcgaccaag atcgagaagg gccgcgcgga
    13501 cctcatcgcg acgctccgcg acggggccga taagcgccgc acggcgcgcg acctgaagcg
    13561 acgtctcgcc gggaccaccg acgccttcgt cttcttccag gaggagcgcg ccggggaaag
    13621 cgcgcgcgag ctcgacttcg agatcatcgg cgccgacgtg gagcggatcc gcgatatcgc
    13681 ccgcgacctc gccgcgcgcc tctcggccct cggcgggtcc gaggacgtgg tgcttcgctt
    13741 caaggagggc cgccccgcag tcgagatcgt ggtggattcg ggaaaggcca tggagagcgg
    13801 actctccgcg ggcgtcatcg gcgatttcgt gcgcagctcc tttcacgggc cggtcgcctc
    13861 caagttcgtg gacgcgcacg aggtggacat ccgctgccgc cttcaggccg acgaggcgcg
    13921 cgtgatcgag cgccttcccg cgctgcccat catcaccgcg aagggcgcgg ccgtgccgct
    13981 gggcgagctc gcctcgttca ggaacgcgtc ggccatcacg accatctggc ggaaggaccg
    14041 cgcgcgcatg gaaacgctta ccgtcacgct cggcgacact gacctcgagc gcttcgtgaa
    14101 gcgggccgat gcggtcatcg ccgcgatgcc gctccccgcc ggttatcgta tcgactacgg
    14161 cgactcgctc gcgaacctgc gccggtcgaa aaaggaactg ctgggcgcgc tcgcgctcgc
    14221 catcctcctc gtatacgcgg tcgtcgcggc gctgttcgaa tcgaccctgc tgcccctcgc
    14281 ggtgctcgcg gcgataccgc tctcgctcat cggcgtgctg gcggcgctgg tcgcgggcgg
    14341 gcgctcgctc aacagctccg tgtacatggg gatgatcatt ctggtgggca tcgcggtcaa
    14401 taccgccatc gtgctcatcg acgcgctcga gaaggaacgg cgtgcgagcg gccacatcga
    14461 ttttccggcg atcgtttccg tcgcgcggcg gcgcatccgg cccgtcgcga tgaccacgct
    14521 cgccgccacg ctcgggctcc ttccgctgct gttcggcggg agcgggagct cgctctggcg
    14581 ctccttcgcg ctggccgtga tcgccgggct tgcggcctcc ggcgtcatgg tcgtctatgc
    14641 cgtccccatc gcctaccacg ccgtgcgcgg cgggcgtcag atgtcgagga tggtttcgaa
    14701 atcggacgcc gggtaatttc cgagtgcgtc ccgcattcca tcggatccgc cggcgacccg
    14761 caggcgaaaa taattgtccg cttcgagatc gaagagatac aggtcgatcg cattgggcgc
    14821 cgccgccggg cgctccatcc tccaggtaac gggcatgagc gcgtgcgtcc ccggcccgtg
    14881 gtagcggtcc cacgcgaagc tgatcgcgcg ctgcgccgct ccccagctca tgccgatccc
    14941 ctgcaggctg ttcgcggtgt tttggaaata cacgcgcagc acgtacaccg ccttcattgt
    15001 gtcgttgata cgatgcatga acccccagta gcgccgcagg tccggcggca gttctgaatt
    15061 atacacgtcg aggtcgatga tgccgtaaca gccgaacacg ctccgcccgg ccttgctctc
    15121 gtccacgacc gcgagggcgg gatcgtcgtt gggcagccat tccatctgcc ggatatcctg
    15181 cacgaagatg gcctgcgaat gcgggccgtt tatcttgaag gaaaagcggt acgcggcgtc
    15241 cagccggttg tccgcgacgt cgatcgcgga atcgcgcacc tccagctcat acacgcggcc
    15301 cagctcaagg gcgccgccac tgagcgacac agtcgcggtg cgcgcgtcgg tccacgttat
    15361 gcgatgtcca ccggacggcg cgggcgtgat ccgcacggcg tcgcccgcct ccaggggctt
    15421 catggctttg gagaagacga tccgcagcgc ggtgtccttc tccacgccgc cggtgagatt
    15481 gcccgcatcc aggcgcaggg gcgcgcccgc gggcggcacg gcgaagacgc ccacgccctc
    15541 atcggccggg acggcgcgcg ccgggtccag cgtcgggacc gcgaaatcgc cgcccaccat
    15601 aaaggagaaa cgcttctcct caaggagcgg gtttcccgcg atgtcggtga tatcgcgccc
    15661 cacgatgacc gagtagccgg tcttgttctc gaaaccggcg tagggggtaa agagcactgt
    15721 cctcccgtcg tcgctcagcg tccgggtccc caatacgccc ggcgagatgg aaaatccggc
    15781 ctccgccgag gagagggcga tgggctccga gaattcgacc cgcacgacag cgttgcgcgc
    15841 gatcccgata cttccgtccg ccggaagcac cgccgtcacg cgcggctttt cgagatcgct
    15901 ccccaccgaa aagtgcgcga cgatctcctc gcggatattg ttgccccgca ggtcctccgc
    15961 cccccgcgac acccgcacgg tataggtggc cccgttgcgc aggtcctcga tgagatcgta
    16021 gtgcagccgg ttcccgtccc agcggaaata gccgcgcgga accccgtact ccgaggagat
    16081 gccgaaggcc tcctcggtct ttggccggtc catctcccgg gggaacgtca cggagatcgc
    16141 cgggcggggc tccacgccgc tcgcgccgtt cgacggatgt acggccccgt tgtccatctc
    16201 gggaaccgtt atgttgagcg cgtcatcgat cggatcggcg ccgctgcagg agatgagcgc
    16261 cgtcatcatc gcgatgaaaa gtattcgaag aagaatctgt aacattgttg tgtgctccat
    16321 ctaatcgtcc gcattgaact tgaaaaaata ccgccgtgtc gcggccatgg gcacgccggt
    16381 aacgcccctg atgccgtttt ccccaccctt gaattcgagc agatacaggt ttcccccctc
    16441 gatcgagccc agcgtgacgg tgaggacgga gtacgggagc gccgcatccc agcgatagga
    16501 gcggacgatc gcgctcgtgt cgtcctgctc gccgttcacg aacgagcaga acagcccgtc
    16561 atgaacgctc acgcggtcca cgtcgcgatc gaacgcgacc ctgaactgat aggtcgaatc
    16621 ggacgagaac tcgacgccca gatcgttgta gtagtcgtcg gccacttcgt ccgcaagcgg
    16681 ggcgtccatg aaccacaccc cggcgacggc gggcggtcgc gatgtctcgg cgtcgatgac
    16741 cgcatacacc acgaagacct cggccatctc gtttccggcg gtatcgcgca gggtccggtt
    16801 cacgcggagc tcatagcgct gcagggacac cagcccgcgc gggaagctga tggtgcagcc
    16861 gtccgaggca tcgttccatt cgacctggtg ttccgcggac ggcgagagcg acagcgcgtc
    16921 cctgaagctc gcgcggtcca ccggctcgct gaagcgcacc tgcaggctgg cggcatggga
    16981 gatcccattg tactcgtgga agctcccatg ttccgccaga tcggtcccgc tcgacatatc
    17041 gacgatcgac gttatgcgcg gccgctcgaa ctcgtccccg gcggtgaaga acgactcgac
    17101 cgtggcgaga agcccgttgc ccgcgaggtc cgtacacgcc gtggatatgg tgatctgata
    17161 gtccgtcgcg aagacgagcg gggcgaaggg gcggtactcc acgaccgtgt cactcgtcca
    17221 gacgaaggtc ccgggaatag acggcgtgaa tcgcaccccg cgctcgacgc tcatgcggtc
    17281 catgggctcg gagaactcga ttcgaatgac gccgtcgagc gggaaggcgc gggtaaggtt
    17341 ttccggcgag atcgagacga cctccggcgg cgcgagatcg tcgcccacga gaaagctcga
    17401 gagatattcc acggtgaggg cgtttcccgc ccggtcccgc gccgccgtcg tgacgcggac
    17461 ggtgtagtaa cgcccgttcg ccagcggcag ctccgggagg aacaccagcc gcctatcatc
    17521 gttctcccac cggaacgatc ccgcgaccat cccctcgctc gaggtgaggc tgaaggcgcc
    17581 ctcggtccgc tcgcggtcca tgggctcgga aaaatccacg gtgacgggcg catcggcccg
    17641 cacggaggcc tggtgcccgg gagagacgcc ctcgatcgcg ggcggcgcga cgtcctgcga
    17701 gcaggccgcg caggcgattg cagcgcacgc catcacccgc acaatccatc ggttactatt
    17761 tagtcgcatc agcattcttc ttctccgtca ccttcatgcc gtcgatgagc atctttacgc
    17821 cgccggaggc gaccacgtca ccctccgcaa gaccggactt cacctcgaag gagtcctcga
    17881 actcgcgccc caggacgatc ttcttccgga acacggcgcc gtccttcacg atgaagacct
    17941 cgccgtctcc gtctttcacg tccttcagcg cctctttggg gaccgtgaca atccccttca
    18001 gcacctccat cgtgatcacc gcgcgggcga acatgccggg gcgaagctgc cccgtgcggt
    18061 tgtcggcgag aatcttcact tccacggtcc gcgtggccac gtccaaaacg gggctcacca
    18121 ggtgcacccg ccccgcgaac ggcgtgtccg cgacggcgtc aaccgtaaag gcgacgggct
    18181 gattcttccg cacgcccgcc acctgctttt cgttgacgtt ggctaccaca taaacctgcc
    18241 gggtgttcat caccacgaac agggtggtct cgtccttcac gcgctcgccg agctccaccg
    18301 agcgcgccgc gacgatcccg tcgataggcg agcggatcac ggcctctttg atagatgtct
    18361 gcaggatctc gatcgaggtc tccgtcttct tcacggcctg ctcggcggcg tccagctcgg
    18421 cgcgctcaac cttcgtgttg agtttcgtca gcagcgcgtg cttctccttc ggatccttcg
    18481 gcacgggaag gcccgccttc atcaggtcgg cgtcgcgata gcccacgcgc agaatctcgt
    18541 ggtccttttt cgcggagagg agcttcgcca gataactgtt gtacgtggtc ttcacggaat
    18601 cgagctcggc atccgagacc ccgcccacga gatgcagctc ctcctttttc cgcatgacgc
    18661 tgcgcatgtt ctcgaacgag acgtacttgt cccgcccgtc gatgacggtt ttcctgatgg
    18721 tcgccatgtg ccgctcaacg tcgcgcccgg cccggtcgag cttctccttc acgaggcggc
    18781 gcgccgcctc gacggccgcg agctccgcgc gggcgctctt gagctgcatc tcgagcggga
    18841 gcgtctcgat gcgcgccagc ggctgccccc gccgcacggc atcgccctgt tccgcgaaca
    18901 gacgctcgac gcggccgagc accttggacg agatggccgc cttgtcccgc tgcgcgacgg
    18961 tgcccatcac cgagagccgg tgctccatgt cccgccgcgc gatcaccgcc gtggcgatct
    19021 ccggcgccgc atcgcgctca acggtctttc gcgacaggag gcgctccgcc atctgcccct
    19081 gaaagacggt tgcggccgtt cccgctgcca tgaccgccag catggccgcg aacacgacct
    19141 ttttgcgtgc ggccaggaat cgaaacactg catgcattcc tttcatcttc atggtgcgtg
    19201 tgccccgctc agctttccaa tatcgtaccc cagcgtccgt tcgaggacgg cgactgcgat
    19261 caggtagtcc gtttttgcca ccagccgccg cagccgcgcg ctcgacaggt ccagataggt
    19321 cttcaggaca tcgtaacgcg tgatctcccc caggcgcgcc ttcgcgtcct cgatcgcgac
    19381 ctgtttctcc aacagcgcga cgttcttctc tgacaccgcg atgcgcgtgg cgttctcgcg
    19441 cacgctgtcg tgcgcccgca ccacctcgat cgcgatggac ttccgcagaa gatcgtgcgc
    19501 caggcgcgcc tcccggtgcg acgcctcggt ccggatgatc gcgctcgcat acgacgggtt
    19561 atcgtagagc cccgcctgcc cggagcgctg cgacgaccgc gtgccgccat tgttcgactc
    19621 cccgtagttc tgggcccccg aaaggctgct cccgaaaatg gcggtcgtga ccgagatgcc
    19681 cacgttccac atcttcttgt tcgggggata gcggttgccc gtgtagccgt agctgcccgt
    19741 aagccgaatg gtgggcagat agtaggcgcg cgcgagctcc atctcccggc cggtcttgta
    19801 cagcaggtag gtgctcttct tgaattcatt gcgcttcgca agcgcgatgg ccacaagctc
    19861 gtctcggccg cgcgtgatcg ccgtgaccgg caccgacgcg atctccatgc gcgtgagcgc
    19921 gagcgcggcg ccgggcgcga gcccgatcag cacccgcagg tccttcaggc ggttctggta
    19981 ttcgttttcc gatttcagaa ttccgtactc ggcctcggcg atgcgcgcct ccacctgcac
    20041 ccgctgcagc tccagactct ccatgagttt tcgattgatg gcgatctcgt cgaggttctt
    20101 caggagctcg taatacaggc tgcgcacgtt caggacgatg gcattgcgct cgatgcgata
    20161 ctcctccgcg gccaggagcg cttcgagctt cgcgatccgg tactccgcgg aggtgcgccc
    20221 gccattgtac aggtccacgc ccacattcaa gaggatcgac tggcttcggt aatcgtcctc
    20281 gcgatacgcg accgtgtcct ccttgctgta gctcacgccc acatcgggaa ggtagctgcg
    20341 ccatttctcg ttgatgatgc gccgcgaaac gtcgatgctc gatttcagcg ccgccagtcg
    20401 cgtgctgcac gtcaatgcgc gcgcgaccgc ctcgtccagc gacaactccg ccgcctgtgc
    20461 gcgagcaccg ctcgtcggtg caatcaacag cagcgctact gcggctgcac acacacgccg
    20521 gcgcgccgat acgccgcccg cgcccatggc tgtcttcgtc atgcacgaca cggccgttac
    20581 cggaaccatc ccgcggggcc cttcaccgcc gcgtcgagcc gctcagcgat agtcgcgcac
    20641 agcgccgcct ggtcgccggg atgggccagg tcgcacgaat cgctcgcggc cttgatggtg
    20701 atcaccgcgg cgccgctttt cgcatcgacc actctaaaaa aagcgacgct cgctgtgcgc
    20761 ggctcgagca cgtcgccgcc cttgacgacg aacacgctca acgccgcgtg aaaggggtac
    20821 tgctccgccc ggtcgccggc gtccagggcc gtccccgccc ggaaggcgcg cttcttgagc
    20881 atgaatccca tggtgcgcat gaggctctcg cgggcattcg tcatctcgtc ggacttcacc
    20941 tccaccgcgc ccagaaacag cgagtcgatc tgccgggttc gcgcgccgcc ctcgtccacg
    21001 gcgtcgatag cgatcgagct gcatgacacc gccaggccca gcaggaagat gcccgcgatc
    21061 cgtatcgcat gtatcgtatt catttccgtt cctccatcca gcgtgtgatg ctgtcgcgca
    21121 tcgcgcgatg cgcccgagcc cgatcgtcca gccccatatg cgcgtagagc gggccgaccg
    21181 cctcgtggat gcgcgacagg tacgcatact gccccagcgc tcgctcgaag gcggcaagcg
    21241 cctcggtcgt gcggcccgca tcggcatgaa gggcgcccag tagatacagg gacacgatgt
    21301 cgtcgggcga tcccgcgagc gcgcgctcaa gaagctgttt cgcctcgtcc gggcgcttcg
    21361 ccctgatgtg ccagcgcgcc tcaaggcgaa gcgcgtcgag cgcatcattc gaacgcgttc
    21421 gatactgctc caccgcctct gcgaactccc ggtcgcgccc cagatgaaag ttcgccatcg
    21481 ccagatatcg ccaggcttcg acatgcccgg gacgcgacga aacgcactcg cgcaggcacc
    21541 gctccgcccc ggcgagatcc cgcttctgat acagcgccag acagtgcgca aaccgctcat
    21601 ccgctccggc tcccccgccg catgagcatg cgaggaggac aatcagcatc acccgccccg
    21661 cccgtatcat ttgaggcctc ccagaaactc gttcacggca tgccgaatga gcgcgcggcg
    21721 gtgatggtcg gcctgccagt cggcgaagga cgcgctgaaa ttgtccctcg tcgcgagctc
    21781 ccatttgtcg gccacccatg cgcgcagctt ttcggagaag cggtatgcgc gggtcgcggt
    21841 cgcggaaacc gcgggcgcct ccgccgacgc cgcgagatcc agcgaggtga cgcacacgct
    21901 cccgctccag agcacgtcat tgttcgccag caccagccgc ccgctgagcg cgccgacgtg
    21961 gagccgatac ggatgctccg ccgccatcgc ctcccccgcg acgaccaccg acacggcgtg
    22021 cgtgagcgaa agctcgcgca cctcgacggt gtcgatgcac agataagcgt ccgacttcac
    22081 ctcaaccttc tccccctcat cgtcgaaacg gaactcgtcg cgctccttct cgtcgttgat
    22141 gacatacccg gcgcgcagca gcgcggtctg cagctcgagc gcccaatccg catgccgcgg
    22201 ccccccgccg ttgttccgcc catcgcgcgg cgcggcaaag cggatcgcga tccgcttcac
    22261 cccggcaatg gccccatcga atgacggcgc cttcgcgcag tctttcttcc ggaattcccg
    22321 atgcgcggtt atcccgcttg tcgtgataat cgattccggc tccggacgct tcgcaacctg
    22381 cgccccgccg ctcgcgcagg ccagccacgc aacggcggcg agcggcgcga gtgcgaatga
    22441 tctcaccaat ctgttcatgg tatcgtatcc ttttctcgac gcatgccgac tcgacggccc
    22501 cgtgggaccg gccggtcatt gtgtgcgcgc gttctaccgt tctatatggt atacgcggcc
    22561 cggcggtttt atttgaaatt ttttaggggg gggtgatggc gtccgcaaaa ccaaacacgt
    22621 gttcatggca aaaggacttg acagctcatg cgatcagagc cagacaatta acccgatacc
    22681 aacgtgaacg tacatcatca cattaggaaa tctcctgcat gagttgtaca ggatttgaat
    22741 cttttgacga ccccctcctg tcctccccct tcgttagggg gaggcgtgta aagagagatg
    22801 ttcctcgccc cctttcccct tacgaagggg aaggctccca acaggatgtt gcagtgccgg
    22861 acaggaggtg ctgaacagga tgttcgaata ccgtcgccgc acaggatgtg caaaaaagcg
    22921 acggcgccaa aaagcgctcg gcgggccggg ggataggggt ataatataac attgacagtt
    22981 ttgattgcag cttcgctgtg ctatggcaat tatatgaaaa tcatcggact ggaaagagct
    23041 atgccttttg ccgcggtcat gctatttctt ctttgtttag cacatccaat tcatgggaaa
    23101 agcgcagaag attatttttc cgatggggcc ataaaagcat ctgagggttt caccaaggag
    23161 gctatcagca actatagcgc ggcaattgaa atcaatcctg gatacactaa ggcttactac
    23221 aaccgggcaa ccgcattttt acgtcttgac aggatcgatg aagccctgcg ggattttaac
    23281 aaggtgcttt ccatcgacaa tcagcatcat cgcgcctatt accaacgagg acgtatcagt
    23341 tatttaaaag aaaagtatgc cgacgcctta caggatttca accgaaccct tgaaattcag
    23401 ccaaccgatg aagacgcatt gcactatcga ggatgtgtgt acatcgtaaa ccttgaactc
    23461 ttgaaggcac ttaaggactt caatacagtc atacaactca atccgagact atcgctatcg
    23521 tttgcctatc acgattggat tctgtacagt cgagataagg gaagaatatt tttttcatcg
    23581 ttttttaatc gggcaataat gaaccgcatt accggagata gggatagtgc aaataacgac
    23641 atccaactga tcaatgataa ttttaaaaga attcgaacaa tcttcggaga taaacaagct
    23701 gacacttcgc tcacagatag gataacggta gcgcttgacg tgtaccaaag ggttgtttcg
    23761 gcacatcctc gatctgctca atcatatcaa ttgcgcggac aagcctatgc gctcctagac
    23821 ctatgcgata aagccatacg tgattattca acttcgatca cgctcaatcc aaaactcgcc
    23881 gctgcatatc tgggcagggg atatgccaaa acagcatgtg gaaaaccgag ggaagcactt
    23941 gaagactttt cccgggcaat tctcatcaaa cccaagttat cggaagcata tatagctcgt
    24001 agtttagcga agcaaaaact tggcgacctg aaaggttttc tcgatgatat cgccagggca
    24061 ataagcgtgt ccccaaaatc aataagggcc cgaaagatcc gcggcgagca ctaccagaca
    24121 actggcaacc ttgcgaatgc tattaaagac tattccgcat tgatctccat caatcctgac
    24181 gacaactggg cttacatcaa acgggccgcg acacaaagca aactcggttt gcacgaaaag
    24241 gcaattgagg attactccaa acttattgaa atgcaaccaa cgcttccata tgcctatttc
    24301 tatcgcggca aagaaaaggc gcatctcgga gacaagcaag gatcactaga tgattattca
    24361 aaagctatcc agatacaccc tgactttaaa gaagcctatc ttgagcgcgg tacaatcaaa
    24421 gcggcaaacg gggacatcaa gggagcatgc gctgattgga acaaggctcg cagattggga
    24481 ttaacaggcg atatcgatga aaaacttaaa acatgcaagt gaacccattt tttactattc
    24541 ttctcgtaga attgaattat gacacccccc gccgtcaccg ccatcatcca gacgcgcatg
    24601 gggtccacgc gactgccggg gaaggcgctg ttgccgctgg cggggctgcc gctcacggcg
    24661 cacgtgatcg agcgcaccat gcgcatcgcg ggcgtggggc aaacggtcat cgccaccacg
    24721 accggcgcgg aggaccgtcc gctcatggag ctcgcgcgcg agatgggctg cgctgccttc
    24781 gccggatccg tgcacgacgt gctggagcgt tactacctcg cctggcgcga gttcggcggc
    24841 gacatcatcg tgcgcgtgac gggcgacaat cccttcaccg acgttgcgta caattcgcag
    24901 gccgtcgcct tcgcgatcga aaccggggcc gacctctgtt cggtggcggg catcccgctg
    24961 ggcaccgccg tggagatctt cacggccgac gcgctcgcgc gcgcctacga ggaaagcgcc
    25021 gaaccgcacc agcgcgagca cgtgtcgccc ttcatcaagg aacaccccga gcgcttccgc
    25081 attgcgcgca agcaaatcga gatcgacaat ccctttcccg gcctgcgcct cacgatcgac
    25141 acgcccgagg atttcgagct cgcgcggtca ctctacgagg cgctctaccg cgacgggccg
    25201 tttccgcttg aggcggtcat cgccgagatc gcgcggcgcc cggagcttgc cgcgctcaac
    25261 gccggcatca cacagcgcgc gatgacgcac tccgagcatg ggacgcgata accggaccat
    25321 cgccatcgtg accgccgcct cgcccgcgct gggttcgggg cacctgcagc gcatgaccac
    25381 gctgttatgg catctcgtcg aagtgagcga tcttgacgct gttgtggtgg cccgcgaggt
    25441 cgggccgttt ttcccagacc cgctgcgccc atatcttcgc gcgagcctgc cggaccgcgc
    25501 cgcgctcatc atccgcgaca tgcgcgactc gaccgaggac gacatcgaac atctcgcacg
    25561 gcgagggccc gtcctcgcga tcgacgacgc gggcccgggg cgcgccctcg ccgactgcgc
    25621 gatagacctc ctgcccaatc ccacaaccgg cgccagcgaa ccggaggcgt tcatctacgg
    25681 ctggggctat gttgattcgc tgcgcgcgct cgcagcgaaa gacgcatctg acatcgcgcg
    25741 cgatatcgac tgcgcgatct accccggcgc cttcgccgac gaagcgcgca tcgagcgcct
    25801 actcgggctc atgcccgcgg gggcgacctc cgccgtcctg cgcggcgccg gatcgttcgt
    25861 gttccgcacc ggggcggaac ggcgacccgc agggacctgc ccgtatgcgc gcctgctcct
    25921 ttcctcgcgc gcgctccttt cgcatttcgg cgtcgcgctc tacgagtccc acgcggccgg
    25981 ctgccgcccg ctcgccctca acccgacggc atatcacgcg cgcctcgcgg acctggcccg
    26041 aacgcggctc ccggtcgaaa acctcggcgt ggacggcgac gacgacgatg ccctgctgcg
    26101 ccgtcggatt gacgcggcgt tggcggcgcc atcaccgcgc gccgtatccg ttcacgaggc
    26161 cttcgacgcc gtcatgtccc ggctggacgc attcgcccgc atcgtccgga cgctcgccgg
    26221 cacccgataa aattctgttg acgccacacc gccgccgcac gatactccaa cgctatcgac
    26281 actgagcacc ggatggacac catgaacagg cttatgcttt tcggattgtg cgcgctcgtc
    26341 gcgctctcct gcgcaaccgc cccgaagggc cccgactacg gcgcgcgcat gctgccgggg
    26401 acctggatcg aggacatcgc cgtcgaatcc gtcttcgagg gggcttcgat gacggtcccg
    26461 gtccgcgtgt acctgccgaa gggatacgac gcgaaaaaac cggtacggac gctcatcggc
    26521 cttcacgcgc acgggggcac caatcgcgat tggagccgtc acccggccat cgtccagttc
    26581 gccgacaaat acgacatcgc cctcgtattt cccgcgatgg ggaccacgct ctacgagagc
    26641 cgctattatc ccgaaacgac caataagtgg aacccgatgc cgggcgggcg ctggatgggc
    26701 gaggtcttgg tcccgtttct gcgcgcgcgc ctgggccttg cgcgggaacg catccgcacc
    26761 ggcattttcg gcaatgcgac gggcgcccgc ggggccgtcc tcatggcctg ccgctatccc
    26821 gctgtcttcg gcgcgactgc gggcatgtcc ggcgactacg acgcgatctc catgatcgag
    26881 gaccggctct tcacctcggt gtacggcgac atgaacaagt tccgccagcg ctgggagaac
    26941 gacgacaacg tgatcaagct tgccgccaat ctcaagggca cgccggtctt cctcacccat
    27001 ggcgagaagg accgcacggt cccctacgag cagtcgaaca tcctggccat ccgcatcctg
    27061 cagctcaaga aggaattggg agactaccgg gtcgagtact acctgcgcta ctaccggttc
    27121 cacgactggg gcctctgggc cgggatgata cccgcgatga tgggcttctt cgacaagacg
    27181 ctcgccaaat gacgccccgg cgcgccatcg cgagcggcgc cgcggtgttc gcgatcgtcg
    27241 cggcgtgtgc gggctgcgcg agcgttccgg ccgtcacggc atacgactat gtctgtgcac
    27301 tacacgggac gcaccatctg cgcgaccgcg cacacaccgc cgcctgtttc ggcttcccgc
    27361 tcgcggagta cgacaatatt cgtttcttct cacgcgaggg cgcggcacaa acggacgcgc
    27421 agtatgatcc cctccgggcg cggctcaccg acgaggtcga aaacgcgatc gtcgatgcgg
    27481 tgatgcagaa gatccaatcg gcgatgggcg cctccatcaa gcgccactgg cgcttcgagc
    27541 agatcaaggt cccggtcgtc ctgcgcgcat tccggcacga ttcgcccggc gcgatccgcg
    27601 cgacggtcgt ggccgcgatg cacaaagacc ggctcgcgcc cgccgccatc atcaaatacc
    27661 tgccgctgga gtataaaatg gacatcatcg actacgaaga gcgcttcaag gataccgcgc
    27721 catgatcacc gccggcatcg acatcggctc catcaccgcg aaggcggccg tcatgaagga
    27781 ccggcagatc ctcggggcca cggtcatctt caccggctac aacgcggagc ttgcggggcg
    27841 gcgcgtgttc gacgagctcc tgggcgagct gggcatgacc gcgcccgagg ttgatcgggt
    27901 cgtcgccacg ggctacgggc gcgcgagcgt gaaggtcgcg accaaggcca tcaccgagat
    27961 cacctgccat gcggccgggg cgcactacct cgatccgacg gtgcggtcca tcatcgacgt
    28021 gggcgggcag gacagcaagg ccatcgtgat cgacgagaag ggcggcgtgc gcgatttcgc
    28081 gatgaacgac aagtgcgcgg ccggcaccgg gcgcttcctg gaggtgatgg cgcgcgccct
    28141 cgaggtcgat ctcgacgcgc tgggcgagct ctcgcgcgcg ggcgagcgcc cggcgtccat
    28201 cagcagcacc tgtacggtct tcgccgagtc ggaggtggtc tcgctcatcg ccaagggcga
    28261 gtcgcgcgag aacatcatcg cgggcatcca cgattccatc gcctcgcgcg tggtcgcgat
    28321 gggaaaccgc atcggcgtgc gcggccccgc gatgatgacc ggcggcgtgg ccaagaactc
    28381 cggcgtggtg cgcgcgctcg aatcgcgcct gggcttcccc ctcgcggtgt cggactacgc
    28441 gcaggtgaac ggcgctatcg gcgcggcgtt tctcgctttt tccctttaaa gagccgctcc
    28501 cagtacgaca cgtcctcctg gctcaccgcc ggatcgtcct ccttctcggg gatcgccgcc
    28561 gcgcggctcg cctcgataat ctcgtcgacc atctttgcga actcctcgct cgtcttcacc
    28621 ttggccttga agcgcgtcac gtacgcgatg atctccttgt cggaggtcac caccgtggcc
    28681 atgcggggat tgatgtcctt cttgatgaat tccttgataa gatggtccgc ggagaaatcc
    28741 agggagtaat agacgtcgat cacgcccgcg ctctcgcttt taatgagcag ggacggctgc
    28801 ttcttgccgt cgaagaccac gcgcaccttc gcgccggtga gccgcgcgta ctccttgagc
    28861 cggtccagga gccccgcgcg cgcctcggcg agtttgccct cgtacatgag gccctcgagc
    28921 tcggggaact tgtagatgag attgaaaccg tcgatcagga ttggcatagt agcgttacct
    28981 cgccccccag ccccctctcc caccggagag ggggtgatca gcaagtagta cttttgtatt
    29041 gagccctctc ctatgggagg cagcaccttg tacatcctag tacggtgctg catacgcgac
    29101 atcctgtcgc taaaggcggc ccggtggtcc ccaagctcta tcgaggggct tgccccgagc
    29161 tcagtcgagg ggaggcgggt gatattattt catcaaaccc gtgcagtagc gctcgatccg
    29221 cttcatcgcc tcggtgaggt tttccatcga cgtcgcgtac gcgcagcgga tgtgccccgc
    29281 gcccgacggg ccgaacgcct cgcccggcac cacggccacc ttctcggcct tgagcagccc
    29341 cagcgcgaaa tcgcgcgacg agagccccgc gggtttcacc gagggaaata cgtaaaacgc
    29401 gccctccgga ttgtggcacg gaagtccgat ctcgcggaag cggctcacta tgaagttgcg
    29461 ccgcttgagg tactccgcgc gcatgcgctt cacgtcgccg tcgcccttct tgagcccctc
    29521 gatggcggca tactgcgcaa tcgacggcgc gcacagcgcc gtgtactggt ggatcttgag
    29581 aatcacgtcg ataaggtact tcgggcccgc cacatacccc aggcgccatc cggtcatcgc
    29641 gtgtgacttg gagaaaccgt tcaggtagat ggtgcgctcg cgcatgcccc ggaccgagtt
    29701 gatcgaaaag cgctcgtcga gataatcgat cgccgagtag atctcgtcgg ccagcacgat
    29761 gatgttgcgc tcggcggcga tgcgcgcgat acgctcgagc gtgggacggt cgatggtgct
    29821 gccggtcgga tttcgcgggt agttcaggag cacggccttg gtctttggcg tgatggcctg
    29881 ctcgacgcgc gccgcgtcaa tggcgaagcc gtcctccagg cgcgtgggga gcaccaccgg
    29941 cacgccgtgc agaagctgca cgttggccat gtacgagaca tagcagggct cgaacatgat
    30001 cacctcgtcg ccggggttca cgatgctgcg cagggcgata tcgagcccct ggctcacgcc
    30061 catggtcacg atgatttcgg tctcgggatc gtacccgacc ccggtttctt tctcgatccg
    30121 cgcggcgatg agttcgcgaa gctccaggag gccccggttc gaggtgtagt gcgtacaccc
    30181 gtcgcggatg gcgaagatcc cggtctcgca cacgcgccag ggcgtgatga aatccggctc
    30241 gccgacgccc agtgaaatgc agtccggcgt ggagtacacg atgtcgaaga attcccgtat
    30301 cccggacggc gggatggccg cgcccaccgc ggagacgagc gattccggat cgtttgtgtt
    30361 gacgattgag agtttcatag tcgtttcttt ttcacgtcct tgggcgcctc gatgagtttt
    30421 cccttcacct gctttttcat ctcgcttatt ttcttcgtgt ccaccgatcc cttgtagacg
    30481 atgaagtaga gcatcgccga ggctagaagc accagcacga ccgcgagcgc cttcacctgc
    30541 ttgaggatga accccgccag gaacacgagc gcgatgcaca cgcccaggaa cacgtactgg
    30601 ttcccctgga tgagcttcac cgcgctgtcg agaaacggtt gcatggccta tttcgggatc
    30661 ttctggtaat ccgaaaggaa tttcgccagg ccgttgtccg tgagcgggtg cttgatgatg
    30721 ccctggaaca ccgagaacgg tatggtcgcg atatgcgcgc cgatgagcgc cgcatgcttg
    30781 atgtgcagcg gattgcggat cgaggcgacg atcacctcgg tatcgtagcc gtagttgtcg
    30841 tagatggcca cgatctcctc gaccacctcc atgccgtcgg cggagatgtc atcgagccgg
    30901 cccacgaagg gcgagatgaa ggtcgcgccg gccttggcgg ccatgagcgc ctgaaccgag
    30961 ttgaagcaaa gggtcacgtt ggtcttgagc ccctcgtccg acaggatctt caccgccttg
    31021 agcccctcct cgatgagcgg caccttgatg ttgatgttcg gcccgatctt cgaaagctcg
    31081 cgcccctctt tcacgatacc gtcgcactcg gtcgcgatca cttcggccga cacggggccg
    31141 tccacctctt tcgcgatctc ggtgagcacc tcgacgtact tccgcccggt cttggccacg
    31201 agcgacgggt tggtggtcac gccgtccagg atgcccagcg ccttcgcctt cctgatctca
    31261 gcgatgtcgg cggtatctat gaaaaacttc atgtggttac ctcgttgcga tttattctaa
    31321 cgccgcatgc cgcaccctcc cggcctggac gacagacggg agactatcgg gatgccacgg
    31381 tgaaatagca agaaaaaaat ttcaatacat cgtcaccgga tatttctttt tcatctcctc
    31441 aatcgacgcc ttgagcttcg cctcgcgctt gagcggctgc atctgctcca cgataagctt
    31501 ttgcgtgtat ggatcgccaa acgggcgcaa gcgcttttcg atcgagttga ggcgcacgat
    31561 ctccacgccg tccggcgtca caaagggcac gctcagctgc cctggcttct gcagcgtaaa
    31621 cccgaactcc ttgtagagcg gaagcttcac cgcggcgttt cgctcgatga cgccgatgcg
    31681 cccgcagtcg cgcgcgtcgg gggcgatgga gtgcttcgcc gccagcgcgc agaacgacga
    31741 cggatcgacg acgatggcct tgtgcaaccg ctcggcctcg gcataatcgc gcacacgaat
    31801 atgcgacacg tcgatccgct gcggctcctc gaactgcgcg cggttcttgt cataaaattc
    31861 gcgcgcctcg gcggggctca ccggataggt gatgctcgat gcgcgccccc cggagagctt
    31921 ctcgaagcca atgtgctccc ggtaggtttc aaccaatagg ttctcccgcg cgcggaccag
    31981 cacctcgtcc aacagcgcac gcgcaggccc atccagtcgc tccgccaggt gcggcaggtg
    32041 cagatccgtc aacgcggccc gcagccgcat gtcgcgctcc tcgggtgaac cggcgatgat
    32101 cccgtcgagc gtgtcgctgt cgagcccgac gaggacgtcc aggagcgtgc tgcgcacgac
    32161 ccgcgagacg atgacgaccg cccgcgccgc atcgcggtcg agcgcggcgt cgcggttcgc
    32221 gtccgtggtg cgcgaccgtc gcgggatcgc tccgcccagc gggaccggcg cccggcgctc
    32281 ctcccggata atggcgtccc accgaacggc cggcgccgaa agctcaaagg tttttacgac
    32341 gagcgcatag agcttcgcgt cgcggtcgtc cgtgatgccg tgttgctcgc acaaaaaatc
    32401 gcggagggcc gccgtccgcg ccgccgcggc aagagcggcg aagccgggcg cggccgcgag
    32461 cccgtcggcc tcggcctttc gcgcgacgag gacattcgcg acgatccggt ccagcgtttc
    32521 ccgctccgac tgtttcgtcc ggtcggccgc catcttcgaa agaacatgcg cgagaaagcc
    32581 ccgtgatatc accgcatcgc ccaccctcgc cgccggcgcg gggtctggtc gattggacga
    32641 gcaaaagacg gtgagtatga tcatgataca gagcgcatac gatgtatact taaccatggc
    32701 acggtgttcc tcccgggcga ttgatatggg cacagcatag cacccgcggt atcggctgtc
    32761 cagcggaaag cgtcaatcat gcggcatacg ccatcatacg acagcgcccg tacgcgagag
    32821 gtcgtattcg ccccttcagc gtcgggcgca taaaaaaagg gcgcccaatg gacgcccttc
    32881 aaagaggttc ctgtggtagg gttaaaactt ccgtgcgctt tccgcaaggt acagatcgta
    32941 tatgtacttc tcgagtacga tcacattgat ggcgatggat ccattatact cctcggcccc
    33001 gttggtgacg cgaacgctgt aatccttcga gtacagcggc gccgggcggt tcacggcctt
    33061 ggtcgcgaag aacaccgtaa tctgctgcaa cgtcacgttc cgcagcttgt ccggcgaggt
    33121 gatcccgtgg gcgttcttga actcctggac gagctccttc acgcgggcga agttcacgag
    33181 gctcgcgcga ttgtcgtcga cccaaccctc gtaatcaacg tggttgtggt cggaggtgtt
    33241 ccacacgtgc tgggtaacgt tggagtcgcc caccatatgg aacacgtgcc cgaggtagta
    33301 caaatgcgcg gcgccgatgt tcgacggcgt gcggccctcg agcgccttcg cgtaccagta
    33361 ggccgacgcg ttcgagttgg gctcgaagat gatgtcctga taatcgtcat agtttccgcc
    33421 cgatgtggtg ctgtagtact tctggccggc ggaaagctga taccggaact tgtatgcgtc
    33481 tttgacgccc ttgccgttgg tcgggtacga aatattgttc ccggcattgc ccgagttgcg
    33541 gacatcctga ttatagagca gcgacttgat gacgccgttt aatccccaaa acccgtcttc
    33601 cagggaatac ttgtagctgt agccgctata ggcgttgcca tagttgcccg ggcggaacat
    33661 ggtgatgaaa tgccagaagc tggtgaagtt ggtgtcgaat ccgaagatac ccacgtgggt
    33721 gcggtatccc acccaccatc cgccgatgga ggtgtcgtag aaattgtcgg tgtcgccgtt
    33781 cttgttgccc atcttaaggg cgatgtcgct gccgccggcg tatttcagga aattggccgt
    33841 ccactgctgg atgtagtcgg catgcgagct ttccatgaat tcgaacgcct tcacgacggt
    33901 gtcgcgatgg gagttaaatt tccatgcgct gccggtgccg ctcagggcaa cacagaacaa
    33961 caggatggtg atgctgatga acagtttcct cattacagat cctccaggac gtgaatacgt
    34021 ttttttaagt acgatcctat cgctattggc tgaattcgtc gttttcaacc attttgattc
    34081 tgccgctgat gaactgccgg ccctttttca tctccgcaag cgccgccttc gcctcgtcga
    34141 tctcggcggt agtgcgcgag ccgtcggaaa gcgccttctc gatgctgtca acggtcgtat
    34201 cgatcatgcg gtcgaagagc gcgccggtct cctcgaggcg gtccagtacg tcgcgcttct
    34261 cctcggcgtt ctcgtaggga cggaaaatga acgaggccct gcgggacaca tcgtccacgt
    34321 cgacggttac ggcgggcgtg ttaccggcgg gagcgccatc gtgtttcgtg agcgcggcgc
    34381 ggtcggcctc gggacgggcg ttcatgaggg tctcgagaat ccgcacctcg acgtccgtgg
    34441 gaatggctcc agccggctgg atgcgttcgg gggccgtttc gcgggcgatg gtcgccgatt
    34501 gtgttgtaac aaagcacaaa ccgcatatag cgataccgac aaccgcgaaa ataactaatg
    34561 caactctctt ggacatacgc ttctctttgt aattagttaa accgtctaac cagactttaa
    34621 tgtaatatac aaaaaactta tcgtcaatag gtatcaccaa aataataatt aatatttcct
    34681 aaaaaagttt gatttacggc taggaaataa tcatataaat atttatgaat ataaaaagtt
    34741 acaattttta ttatctccaa actattcacg catactcttc gaattatgtc ccatgaatta
    34801 tttcgattga ctcgaccgcg ccgatatcgc tactctgagg atattccatc gagctggagc
    34861 acgcatggct gaaagcgatc cgcggaccga actccttgag cgcttcaatg aacgacgcgc
    34921 gaaatggcag cgccagccga gcgcgcccaa gcgccgtttc gccccctggc tgcttctcct
    34981 caatctcatt atactgggcc tcatggtgtc gtatcactac cggagcgcgc ccgatcgcac
    35041 catccatcac acgctcgttc gatacgccgg gaccgcctat cggctcacga taacgcacga
    35101 cgagtccgca aagaccatcg acgtgacgct caccatgaaa aacgagcggg cgatccccca
    35161 gcggctcttc tttctcgatc ccgtcgccgt ggcgaccttc cgacacgacg aggcgaccgt
    35221 gctctcggtt ccgctgggcg cgggtacgcg ggacctctcg atcaagccga acgactcgcg
    35281 ggtgttcacc gttcgcctgg gcgaagcgca cctgcgctcg tttgccgagg cgaacccgga
    35341 cgcgatcgtc ccccggcgtc ggacgctttt ctcggccaag cggcatatcc cgctggccgt
    35401 ggagctgtcg cttcgcaccc gcgacgcact cgcggcgtac ctggacttca agtacgtgat
    35461 cgagtaaaca cgcggataac gcttgacgcc gcatcatcgt gcggcataat agagaatgtc
    35521 ttgcaggcgg cccgccttcg gcgaaacggc tgcgggcgcg aacaatcgtc acacaagcga
    35581 ggttgatgat gagaacgcgc atgttcagaa tcctcccgat tgtcatcgtt acgacgctgg
    35641 ccgtgtcctg cggggacatg aaatgcacat cgagccttcg cgagcggaag accgacgacc
    35701 agatcgtaaa ggaattgaag gacaagatcg cgcgcgagct caaaaaggac gaatccagcc
    35761 gccagcacca gcgcctgctc gactggtacg aacagttggg cgatcgctac ctgatgatgg
    35821 gcgtctggga acaggcgatc gtcaacctcg agaaggtgat cgcgcaggga cggcagagcg
    35881 ccaatctgca ctactcgctg ggcctggcgt acgccaaccg cggccgtgac ctcaaccggc
    35941 cggaggattt cgaccgcgcc gagtcccagt atcggcgctc cctcgactac ggtatggtcc
    36001 gaaacgaggc ggggttcgcg ctggccgtgc tcctgtacta tcagaaaaac aagaaggacg
    36061 atggcctgcg gctcatggag gagttcgctt ccaagaacaa aaaacactac cgctcgcgct
    36121 tcggcctggc gcggatgtac tacgacagcg gccgcccgga ccagtccctg gcgacctacg
    36181 aggagctcta cggcgacctg gagaagctac ccgactcgaa cgtcaagaac gagtacatcg
    36241 aggcgtgcaa aaataacatg cagcggctgc agggcgagct cgctcggccc ggatcgaaca
    36301 agtaaaaaaa cccgatccgg gcgatttata ctatcaaaca atccgcatcc gccaaccgat
    36361 aataaactat cgtagcaggt tcaatccggc ttcgcgaaag cacgtctccg gggaaacgat
    36421 atgagcttaa cctatattct tggcgtcacc ttcggcatgg tcaccctggt gttcggtatt
    36481 ttttcagcgg ggggcaatct gctcatgttc gccgacctcc cgtcggtatt tatcaccgtc
    36541 ggcggctcca tcgcgtcgct catggtcggc taccccctgg tgcgactgct taagatcaag
    36601 gacctgttcc ggatatgcct attcgactcg aagttcaatt attccgaact catcctcacg
    36661 ctagtgtcgt tctccgagaa gtcgcgccgg gagggcctgc tcgcccttga ggacgatctg
    36721 caggacctca acgacccgtt catgaaaatg ggcatccagc tcgtggtgga cgggaacgac
    36781 cccgaactgg tgcgtaaaat attggaaatt gaaatcgacc agatggccca gcgccacgac
    36841 tcgtacaagc gcatgttcga cgattgggcc accctggcgc cgtcgttcgg gatgatcggg
    36901 accctcatgg gtctggtcat gatgctcgta aacctccagg acaagtccac catcggcccc
    36961 tatctggccg tcgcgctcct cacgaccctc tatggcgccg tgatgtgcta cgtcctgttc
    37021 acccccatgg cgaccaagct cgacatgaaa acgcaggaag aggtgatcgt gaagatgatc
    37081 acgctcgagg gcatcctctc gatccagtcc ggggaaaacc cgcggatcgt caaggacaag
    37141 ctggtcatgt acttgccgcc gtccgagcgc gaggcgatct cacgcgaggt cgaatagccc
    37201 gtgggaaaga agttcgaaaa gtcaaagcag aagggcgccc cgccctggat ggtctcgatg
    37261 ggggacatga acaacctgct catgtgcttc ttcatcgtgc tcatgggcga ggacgtggtc
    37321 aagacccagg ccgaggacat tcagctcatc atgtcggcca tcaaggggaa cgtgggcatc
    37381 atggacggcg ggaagacctt cgctcgcggc aagctcgcgg agatggggca caatttgctc
    37441 tcgctgccct ccacgacgcg cgagcgcacc ttcgccaagg gcgtcaagcg cgcccaggag
    37501 ctgttccggc ccgagatccg ggccaagcag gtccgggtca cggaggacga gcgcggcctg
    37561 gtgatttcgc tctcgtcgga catctatttc gactccggca gctcacgcct caagcccgag
    37621 gccaaaccgg tgctcaaaaa gctctccggg ctcttcacgg gcatcacgaa ctttatccgc
    37681 gtggagggtc acacggacag ccgacccatc ggcacgccgg gcgtgcgcga gggcttcgaa
    37741 tcgaactggg acctctcctc ggcgcggagc atcagcgttg tgcgctattt catcgaggac
    37801 gagcgcgtca accccaagca gctctccgcc gtggccttcg gccagtaccg gcccatcgac
    37861 gacaacaaca cgccggaggg gcgcgccctc aaccggcgcg tggacatcgt gatcctccac
    37921 gaccgcatgc tgcaggaggg caaggacccg cgcatctcgc gcccgctgcc cgacgaggaa
    37981 tggcgctagg cggcggacgc cgctagtcca tgcgctcgac gatgtacttc ctgatgatgt
    38041 ccgccgcgat ctcagaaatc tccaggaact tgaaggtgta gtagtgcatg ttctcctcgt
    38101 ggatgacggc gtccgccatc gtcttgaggg gtgagcgtat tttgaaatcc gacagcgtga
    38161 agtcgatcga atactcccgt tcgaaggcga tggggcttcc cacccgcacc acgatctcat
    38221 gctcgttcat tttgcagatg gtcccgcgga ccgaatccgt ttcctccgcg ccgccgccct
    38281 tttcgcggtc gaccggccac gacagcgtgc acgggatcat cacctcgaga taggggtgac
    38341 gcacctcgcg cccacgcttg aaaacgtccg ggatgaggat gcgcacgccg ttcccgtccg
    38401 attcgctcac cgtggcctcg aaggtgtatt ccgcgtcgcc gtttcgccac acgaacacgc
    38461 tcaccttctc ctgcagcagc tccccctcct tgagctcgcg atagagcgtg agcgcaaggc
    38521 tgctttggtc cttggcggtc agcttcccga caaacacgcg atcgtcctcg gtctggaagt
    38581 acatcaggct gtcggtctcg agatcacgga tcgaatccag cggcttgcga aaggcggccg
    38641 gatgatacag cttctcatac gttatcacga tgtccttgcc gatcgcaagg agcgaatcga
    38701 cgctctcgtc ctttttgtgg atgtagctca tgaacgacgc gatcgacgac tcgaagagcg
    38761 agggcgtgtg gagcacggcc cgatagtcct tgaggcccac gatgcggacg atgcccagca
    38821 ggattttata ctggaagttg gtgagcccga gttgcttggt cttgaaatta aagaactgtt
    38881 cctcgtggcg ctccttgtcc ttgaggtact ggtaatagcg aagcagggac gcaaacgcca
    38941 cgacaagcgc cgccaggcca atcatgagcg cgatatcgaa accgctcgcg acgggccact
    39001 cgaatccgaa aatcgtttgc atgaggcctc ctcacgcgac gacggtctcg cccgcggaat
    39061 atgccggcga ttcggtcccg cgaatgaccg catcatccca tatccgccgg cagaaatcaa
    39121 tcattttcgg aaaaagcgga aacggccgca tgctgatccc gccgcgcacg cgcgtcggcg
    39181 ttgagcgacg gaccgcccgc ctagtagagc ggcttgtagt cgaagtggtg gcgcgtgttg
    39241 acgaagcgca cggtgtgcgt cttcgagcgc atcacgatgg agtgcgtttc ggcgccgccg
    39301 ggccggaacc atacgccggg caacagctcc ccgtcggtca cgcccgaggc cgagaacatg
    39361 atgttcttgc cgggcaccag gtcaacgatg ccgtagatgc ggtccggatc gtcgatcccc
    39421 gaatcgcgga tgcgggcccg gtcctcgtcg cagcgacaaa caaagcgcgc ctgcatgtcg
    39481 ccgttgaggc acttgagcgc cgccgccgcg agaatgccct ccttggcgcc cccgacgccc
    39541 atcagaatgt cgatcgattt gtcctccatc gccgtggcca cggcgccgga gatgtcgccg
    39601 tcgcggatca gcttgatgcg cgccccggtc tcccggattt tactgatgag atcgacgtgg
    39661 cgctcgcggt cgagcacgca cacggtcacg tcctcgatgt atttgccgga ggcccgcgcc
    39721 acgcgcttga tattgacgct cggcggttga gtgatgtcca cgacgccctt tgcctcgggc
    39781 cccaccgcga tcttctccat gtagagttgc ggcgcattga agaacgcccc ccgctcgccg
    39841 atggcgatgg cggcgatggc gttgtgtccc ccctgcgcgc aggtggtctt tccatcgaga
    39901 gcgtccacca cgatgtcggc ctcgatgccg ttcatcgtac ccacggcctg gccctttacc
    39961 agcggcccgt ccgccgggtc ctgttcgccg ttgatgacct tcccgttgat ggacaccgac
    40021 gagaacacct tggccatcgc ctcgatcgcc gagctgttgg ccagcatctc atcgccgcgc
    40081 cccatatagc ggcttgcgta cagcgcggcc gcctccgtca cccggaccag ttccagcgcg
    40141 agattcctgt ccatacagcg cacccccttt atggcaaaac ggttctcacg gagccgatga
    40201 ccgcatccaa aaccggattt taaatatttc aaacaaaaaa ttcaaaatcc ggagggcgca
    40261 cccgtatgcg tcaggttatt gcttcgcccc gataaccttg atctccgcct cgattttttt
    40321 gcactcgttt tccgccgcga gaattttatc catcgtttcc ttcacgacgg cgtcggcgta
    40381 cacgtccttt ttttccttat agagatcgca cacgacatcg ccgagatcgg caagcaggtt
    40441 gtgcagtttt ctttgcgcga ggataaggtc cttcttcagc tgcacatagg ccataccctc
    40501 ctttcccttc tcgacggcga tcgcggcccc ctccttgatg ttttgcgccg cgagatccag
    40561 ccccttgagc gcaatctccc gaacttctgc ccagtaaccc atcataaacc tccagaattg
    40621 gcatttccgc gcgcccgcat gtcggacgcg ccatacccgt tcagtatacg gtacaatgac
    40681 gccaatgtca accactccgg gagatttgtg cggcctaatg atgcgcctcg ccatgcttta
    40741 cccgcaccgg catcagggcg atcgcgcgac cgagcgatca atcaattgcg cacaacgcta
    40801 tttccgcggc tttgcgcgcg ccgtgggcgc ggcggcgcgc ggatcatccg gccaaaaatg
    40861 gcggggatag cggcccctga gctccttttt cacctcggga taggcgcggt cccagaagcc
    40921 gtcgaggtcg cgcgtgatct gcaccgggcg gcccgcgggg ctcaaaaggt gcaggatgat
    40981 gggctcgccg cagaggcgcg gggtctcgac gctgccgaag aattcctgca ggcgcgcggc
    41041 gaggacggga atgtcacccg cggtgtagtc gatcggccgc ctgcttccgg acgggagggt
    41101 ccatatctcc ggggcgatat cgtcgagcag gcgacggttt tcccatccca gacggccctc
    41161 gagagcgcgc acgatcgagc gctcgtcgaa caccgggccg cccgaccacg ttccatgcgg
    41221 cacgagccac gaatcaacct cgcgcgaaag cgcgtcgtcg gaaaaatccg gccaatcatt
    41281 ccgcacgccg cggccgtgga cgaaccggca gcgcgcacac aggcgccgcg acgtttcgcc
    41341 ccaggggagc gattggattc cctcgcgcat gatccgctcg cgcaccgccc gttgcagttc
    41401 ctctgcggcc ggactgcctc ccgattgccg ggagagaacc agcgccccca ccctccgctc
    41461 aacgtggacc gcgggcgtcc agccccgcca ttcgaatgtg caacacgcgc ggaccggtcc
    41521 ggctgcgcca ccctcgagat cggcgcgcgc gatgggtgcg gcaagcatca cgcgcccgtc
    41581 gctctcaccg ccgtcaatct ccgcaatggc tagaaattcc tcggcggcaa gcgcgccgcg
    41641 caccgtggcc ccgcggccgc ccgcgagcac cagacgcgct cgggccgggc cgtcgtcgcg
    41701 tgtttttcgc gctgcgcgat ccggatacgc gccaagcagg agaaggccgg cgagattcgg
    41761 atcgatcgcc tccgcgtcga tggcgctgca gcgtaccccg gcctggctta aaagacgggt
    41821 cgccgcatcc gcgatccgcc ccgcaccgct ccccgagaga cggccgcggg aaccggtgag
    41881 ccattctcgg caggcgtcga ggcgggtccg gagatcgacg tcggcgccgg gaagcgggtc
    41941 gccctcctcg atgatcgcgg cgatgatcgc ggcggtcgcc gccgcgccgc ggtcgatgcc
    42001 gtcgaagagc atgcgcgcaa aacgggggtg cgcgccgacc cgtgcggccc ggcgtcccgc
    42061 gtcggtgatc cgtccgcgcc cgtcgaggaa gccaagatcg gaaaggagcg attgcgccct
    42121 ccgcaccgcc ggttccggcg gcggcgttat ccaggccaga tctgagggcg atggtgcccc
    42181 ccagtgcgca cactcgagca cgagcggcgc gaggtccgcc tcggcgatct ccggaggcgc
    42241 gaagtcggac cgcgactccc gtgaatccca ccagcgcagg catagccccg gacccagccg
    42301 gcccgcgcga ccggcccgct gctcggccga cgccgcactg atcgggacgg tttcccagtg
    42361 gcccatcccc gttcgcggcg aaaagcgcac gcgccgctcg agccccaggt cgacgacggc
    42421 ttgtatcccg gggatcgtga ggctcgtctc ggcgacgttt gtggcaagga tgatccgccg
    42481 ccggcccgtt tcggggcgtc gcagcaccgc gcgctgctcc tcgggccgca cgcgaccgtg
    42541 cagcaccatg atctcggcgt cggccgacga aaggccggcg cacgcctccc gcgcccggcg
    42601 gatctcgcga aagcccggga gaaagaccag cacgtcgccc gcacattgta tttcgcgcaa
    42661 cgcttcaagc gcgaggcgac cggcggcgtc ccatggggcc tcggcgcccc gcgggggcga
    42721 ataccggatc tcgacgggat gcgcgcggcc ggggatcgac cggtgcggga catcgcccaa
    42781 tgtgtcgcgg agcgcgtcgg cattcatcgt cgccgacata aaaagaaagc gaagatccga
    42841 ccgtaggtgt tttttgcaat cgatcgcgag cgcaattccc aggtcgccga cgaggctccg
    42901 ctcgtggaac tcgtcgaaga tgatcgtccc gtaaccgcca agggactggt cgccctggat
    42961 gatgcggacg aggacgccct cggtcatcac ctcgaggcgc gcggccgggc ccgagatcgt
    43021 ctccgttcgc gtgcgcaacc caacggcgct cccgatctcc tcgcccagaa gcgcggcgat
    43081 ccgctcggcg gccgcgcgtg cggcgatccg gcgcggctgc aggagcgcga tcttgccgcc
    43141 ggcacactcc ttctcccgca gcagacgcca cggtacgagc gtcgtcttcc ccgcgccggg
    43201 ctcggcatgc aggagcatcg cggaatggcg cgcgagcgtt tcggcgatct cgtcgaggtg
    43261 cggataaatc gggagggcag cgatatcgtc aggcgacatg cgtacgatgg gtcgggacgc
    43321 ggatgcgcga tgcggcgcga agcggcatca gccccctacg ccaccgtcac ctcagcgcaa
    43381 agatacacgt cctggatggc gttaaggagc gcgatcccgt ccttcatggg acgctggaat
    43441 gccttgcggc cggagatgag ccccatgccc cccgcgcgtt tgttgatgac cgccgtgcgc
    43501 accgcctcgg cgaggtcgcg ctcgccggag gactcgccgc cggagttgat gagcccgatg
    43561 cgccccatgt aattgctgat cacctggtag cgcgtgagat cgatggggtg ctccgacgag
    43621 agctccgtgt acaccttgtc gtgggttttt gcgaagttga tcgcggtgaa cccgccgttc
    43681 agcgtgggga gcttctgctt gacgatgtcg gccttgatgg tcgcccccag atggttcgcc
    43741 tgccccgtga tgtcggcggc ggtgtgatag tccgcctcct tcgtcttgaa ggcgttgttg
    43801 cgcgtgtagc accagaggat cgtggccagc cccagctcgt gcgcgcgctc gaaggccgcg
    43861 gagatctcct ggatctgccg cgagctctcc tccgaaccga aatagatcgt cgcccccacc
    43921 gcgacggcgc ccatgtcgac ggcctgctcg acgctggcga agaggacctg gtcgtacttt
    43981 gtcggatagg tgagcagctc attgtgatta atcttgagaa tgaacggaat cctatgggcg
    44041 tacttccgcg cgaccatcgc gagcacgccc agcgtggagg cgacgccgtt gcagccgccc
    44101 tccagcgcga gccgcacgat gttctccgga tcgaagtatt cggggttggg cgcgaacgac
    44161 gctccggcgg tgtgctcaat cccctggtcc accggcaaaa tggaaaggta gccggtgttc
    44221 gcgagccgtc ccgccgcgaa gagcgcctgc aggctccgca gcaccgggac cggccggtcc
    44281 gacggcgcga acacgcggtc cacgaagtcc ggccccggca gggtgatccg ttccttcgga
    44341 acgcgcgccg catacgtcag cagcgattca ttgtccgatc ctaacgcttt tcgaattctt
    44401 tcaatcatga tatccctccg cgtttcagat gcacgaccgt ccgcctacct gaatttttcg
    44461 gttataaaaa aggcgtccgc gatcggcaca tcgtactccg ggttccccgc gagcttcagc
    44521 tcggtcttgc cgcgcccgtc ggcccggtac atgatgatgc ggtacggcgt caggtaattg
    44581 ccaaccggct tgacgttgat ccgctcaaga tacttgtgga attcgtcctt gagatagtaa
    44641 tcgatgcgga caatgtattg atcgtaggtg cgcaggtaga gcaccagctt gctgtacacc
    44701 accgtgccgc gcttcttgat ggcttcgatc ttgtggcact cctcgcccac ggccttcacc
    44761 gtcccgcggt gcatgaagtc gtagtcgtcg acgttgagct ccttgaggtc ctcgaagtac
    44821 aggttcgaat tggcgaacgg cttgtcgagg tcggtgccca cgatgcgctt cacgttcccg
    44881 gccgacatct tgagccactg atcgtcgtcc cggtttttgt gggcatggct gaggagttca
    44941 agccgcgcgg gatgcgtgaa tgtgatgcgc atcttgttct cccggtccgg gttccgtttc
    45001 acgtactggt caaagtgctg ctcgagcacc ttctcgcccc tgaacaccag catcttgtac
    45061 cggaacttcg ccgtctgcgg ctgcgcgagt gccatgcttt ttcggaccag gtcccggccg
    45121 tcgtcgtttg cataaagcgc tagcggcacg ccacacgcgc acacggccac catcaggaaa
    45181 cgtcgcatca tgataagtcc tcccggaaaa aatatctgag catcgcatcc tcccatggac
    45241 gagaaaaatg cacaatactt tttttcgcgc acgtcccgcg ccggcccgac gcgccggtat
    45301 tctgcttaat attttccctt tgcgaaacga tgatacaaac agacccccca cggaggatcc
    45361 gcccatgaga aatgcgctct gtgccatctg catcgcgctc gtatcagcga cctgcgccgg
    45421 tcgcgacgcc tcgatgcgcc gcgagacgac ggccattcga cccgacagcc tcgtcaactt
    45481 cttccgggtc tgcgagcagg acagcaaaaa cgcgatcgcg gtgatcgtca atcgcgacat
    45541 caccaagaac ctgacccatc tgcagcgcat ggacggcggc gggcggaagt actatctgtt
    45601 ggagcgcgag gccatcaccg agatgatccg cgcggtcacc tcgggactct atgacgactt
    45661 catcctcatc aaccgccacg gcgtcgtggt gtacacaatg gccgacgacg ccctgttcgc
    45721 caagagcgtt ccggcatcgt tcaaggggac ggcgctccag gagtgtttcg agaaaaccgg
    45781 caccgatctg tacctgagcg acacgggccc gtcccccggc gcatcggagc gtcccgtgct
    45841 ctatgcggcc gggcgcgtgg tccgcgacgg cgaatttcac ggggtgtttc tactgcaggt
    45901 gcgcgccgag cggatcgcga gactcatcgc ggaaaacgaa tccatcgtga gtcgggccgg
    45961 gatcgtccgc atttcgcgta acggaattcc agccgatgcc ccctacccgg atttcgagcg
    46021 catcgccccc gcgaccctcg tcgaggcccg cagccaccgt ctcacactcc cgcgcgcggt
    46081 cctctcgtat tatcccttca cctacaaaaa cctctcgtgg atcatcatcg cgcgggagcg
    46141 ctaatcagtc cggcgaggcg atcgtggatg ccgccgaaga gctcggggag gtaggccccc
    46201 ggggaggcgc tctcgacgag aagccgtgac gcagcgatga tacgcccatc gtcgatgccg
    46261 cacaaccccg cccggtgcgc gcccatcagg aggcccggga gcaccgatgc gcggtgctgc
    46321 tcggagacga cgacgtgcgc gcggacgccg cgctcgcgga agacggcctc ctcctcggcc
    46381 gtaaccggga cgagcgatat gaccagctga cccgggcgga tcttgcgcag atcggcctcc
    46441 gtaacgtcgc gcgcgccgcg cacgacgata tcggcattgc cgaagatgtt ctcgagcgtg
    46501 gtcgcaagcc cccccgcctt ctccagtgcc agcatcaggg tctcgctcgc atcgcagccc
    46561 agtatccgcc ggtagccccg cgccgcaagc aggtgcgtga gcctgatagc cgcgcaatcg
    46621 atccccgcga accccacgct ggtctcgccg cgctcgagcc cgtttttttc cgagaggccg
    46681 tcgatcaccg ccagcaacag ggccggcgtc tcgtcgtggt cgcgcgtgac aagcgggacc
    46741 gtcaccccgg cgctcacctg ctccagatcg gacgggtcct cgacctcccc gacggacgag
    46801 atgcgcacgg cggcgaaggt cccctcaatc gcgcgaagcg atttcacgaa atcctcgatg
    46861 tgatcgaaga cgatcaccag cggataggcg ttgaggccgg cgaaccggga cacgacgagc
    46921 gcgtcccgct cgtttcgcac gtactcctcc gccaccggcg ccgtcccgcc cccgggattg
    46981 gcccggacgc cgctcaccac cgcaacgttg cgcgcgattc cggtgcgcgc cgacccgccg
    47041 gaggcggcgc gttccaacag gagcgccgtt gcgcccaata ccttcgtttc gaaatcgtcc
    47101 gcctgctcca gaggcagccc ccccgacacg cgcaggagcc cgccctcgat caactcgtcg
    47161 agcgcgttgc ggaccgatac gagctcgaag ttctcggaaa accggccgat ccgctccagg
    47221 aaggccgcaa agccctccat cgagtcgcac tgcacctcga tggcgaagag gtccccgccc
    47281 ccgtcctgct cgagctgcgc gagcgaacgc atctcgcagc cgcgcttgta gagctcgttg
    47341 gtgaggtggg ggagaaagcc gcgcttgatg cgcacggata tttcagagag tagtttcatg
    47401 gcgccggtcc ggtcagcggg agatatcgcg tcgtttgtaa tatcggccgt tttcggccgg
    47461 gagtttacgc ccgaccggac atcgcggtcg cactgcggat ccccgccgtt tcggcgcgcg
    47521 cggcttccgc gatatgttcc ggggacgcgt cggggtccgc gcggacgagc agatagtccg
    47581 attcgagggg acggcacccg atcgagttga agaaatgccg catggtcacg accgacggct
    47641 cgaactggcg gggatagtcg ccctcgccga cggaaatgaa gaacccgtcc cgcgcgccgc
    47701 tgatgcactc gcggcgcgca agctcccaga gcgcctggca gcgatcaatc acggttttca
    47761 gcgggccggg aaggccggag aagtagaccg gcgacgacac gctcaccagt cgggcctgca
    47821 cgagcagggg atagagcgcc gtcatctcgt cgtcgtgcac gcacgagaac gaatcgcggc
    47881 aatgcccgca ggcgatgcag ggccgcacgt ccatcgcgta ggccgcatac cggcgcacct
    47941 cgtgtcccgc cgctgcgagc ggggcgagaa accgttcgtg aaggtgcgag gaaacgctcg
    48001 tgtcgcgcgg gcttccaaac agcgacagga cgagcgccac cgcgtcagtc ctcccggagc
    48061 cgtgcgaaga tcatgcgtcc ggccgtggtc tgcagcaccg aggtcacgtt cacgtgcacc
    48121 tccgagttga gccgggagcg cccgttctcc accaccacca tcgtcccgtc gtcgagatag
    48181 ccgatcgcct ggttggggtc cttcccttcc ttgatgagcg aaacgcgcat ctcctcgccc
    48241 ggcagcacga tgggcttcaa ggcgttcgag agctggttga tattaagcac gtccacgccg
    48301 tggaactccg cgaccttgtt gaggttgaaa tcgttggtga tgatcttcgc cttcatcgcc
    48361 ttggcgagct tcacgagctt cgagtccacc tcgtgggtct cggggaagtc catgtccgag
    48421 atcttcacca tgatggactg atccttctgc atcttattga ggatgtccag gccgcgacgg
    48481 ccgcggttgc gcttgatgga gtcggccgag tccgcgatca tctggagctc acccagcacg
    48541 aaattcggaa tcaccaggat cccctcgatg aagcccgtgt cgcagatgtc cgctatccga
    48601 ccgtcgatga tgacgctggt gtcgaggatc ttgtaagcga tcgtgccgcc gcggtcgtcc
    48661 tccgcgcgcg cggggacgat ggcgtcgagg aaccggatgt cctcgtggtt gatcacgaag
    48721 aagagcagca ccgcgaaccc gctcacatga tacaggagcg cgcgcacgac cgcgagggtc
    48781 tgcgccgaga agggaagcgg cagcgcggag aaagccagcg ccagcaggtg ccccacgacc
    48841 aggcccgagg cgagcccgat gatggccgcg aagagcagcc gcgcggagaa ggtgtgcgac
    48901 acgtattcga tggcgatgat caccaggagc gacacgacca ccgacaccac ggccgccacg
    48961 agcatcgcca gccatgacac ctcgagatag tacagcaccg aacagatcag cgaaagtgct
    49021 atgaacgaac cgcgtaaaac attaatcatg accggacacc tctttctgtg aatagtgata
    49081 ttctaccgcc acccgtcgtg tcgcgccgaa aaaaacgctc acaccgcgac ggacgacgca
    49141 cataccgaca cttcaaacgg cgcgcgggcg cccgcggcga tgggaccgat gccgcgtcgc
    49201 ggggacgggg gatgcgtccg ggggttatac ggacgagagc gcttcggaga tgaggttgcc
    49261 cgcctcttcg accgcgacgt ccttggtcag ggcaatctca tggatcaaca gctgataggc
    49321 gttttcgtag agtttgcgct ccataatgga gagctccttg ctgcggcccc gccggtacag
    49381 gtcccgggtc acctgggcca cctcgaagat cgaaccgctt ttcaccttgt cgatgttgtt
    49441 ctgataccgc aacttccagt cgtcctcggt caccacgttt tcctgcttga tgatcgtcag
    49501 gacctttttg atgtcttttt tctggatgat gggccgcagg ccaatgcggg ccgcgttgtc
    49561 aaccgggatc ataaccttca tcccgctgtt gatgatgttg atgatataga actccgtttt
    49621 tttccccaga accacttttt tttctatcga gtcgatcctg ccgaccccat gcatcgggta
    49681 cacaaccttg tcacccagct gatacatatg gttctcctgt ccctagtacg cgcgcttccc
    49741 ccgcttccat gcgtttcgcg gtcgttcgag aaatgtataa tggcatcgga cccccgtccg
    49801 atttggcgac ggtaaaaaaa agtggccaca aatcaagaat aattatcacc gtcggccggt
    49861 tttattggtt ttcgtcgagc gatatcccct cgcgctccat gtagcgctcg agcaccgact
    49921 tcatcttgga gcccttgaag tccgaatacg gcccgatcgc gccgtggaag accccggacg
    49981 gctccttggg aaacgccgga tcgggcatgc cgttgtagac atccttcatg tagcgccccc
    50041 agatgggcgc cgcaaccatc gcgcccgccg cgcccgcgcc cagcgtcatg gtcgccttgt
    50101 cgtaccccac ccagacgatg gccgcgatat cgggggtgag tccgcagaac catgagtcgg
    50161 accagttggt ggtggtcccg gtcttgccgg cggcctgctt caggaagccc gccttgtagc
    50221 ggatgggctc attcccggtc ccgccgtcga tcacgccgcg catgaggtcg ttcacgagcc
    50281 aggcagtctg ctcggagatc acctgcaccg tgccgtcgcg ctccttcgcg gctatgatgt
    50341 tgccgacctc ctcctcgacg ttcatcacct cgttgccgtc cttgtcgatc acgtagcgga
    50401 tggcgaaggg gatcacctcg cgccccttgt tggcgaagat ggcgtaggcg gtggccatct
    50461 cgaagggcgt cacctcggtg gtgccgagcg cgagcgtcgg attgggatcg aaccggcttg
    50521 gcgggacctt cagcatcttg gcggcgaagt cgatcacgtt gtcgggaccg accaggtcga
    50581 agacgcgcac ggcgatgatg ttgatggagg ccgcaacggc ccgccgcacg tctacgagac
    50641 ccttgaagtc gccctcgtag ttggtggggc tccagcctac gccctcggag tcgatctcga
    50701 cgatgggcgc gtcgggcagg acggagcggt cgctcacgta ctcgccctcg atggccgcgc
    50761 cgtacacgaa gggcttgaac gacgagcccg gctgccggcg cgcctgcatg gcccggttga
    50821 actggttgtc cactgtgaag gtg
//
LOCUS       DAORHF010000004        40004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_496_length_40004_cov_5.601968, whole genome shotgun sequence.
ACCESSION   DAORHF010000004 DAORHF010000000
VERSION     DAORHF010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 40004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40004
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_496_length_40004_cov_5.601968"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1696
                     /locus_tag="PKJ16_00780"
     CDS             <1..1696
                     /locus_tag="PKJ16_00780"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HNV45545.1"
                     /translation="NFNLSVAITDEFMRAVRENRDYELVSPRTGRPVAVRNARAVYAA
                     IVEHAWHSGEPGIVFIDRINAANPLKRVGDIESTNPCGEQPLLPFESCNLGSINLSRV
                     LTSTGGAYEIDFPRLGEITRAAVHFLDNVIDRNRYPLPEIAESTLANRKIGLGVMGFA
                     DTLIMLGIPYDSEEAIETAERIMSFIQDESKAASRELARERGAFPNFERSSYAEAGEP
                     PLRNATTTTIAPTGTISIIANCSSGIEPIFALAYVRNVMDNNTLVETNPFFRSYADAN
                     GFYSDELMTDIARHGSVRDIDTVPEAARRLFVTAHDITPEWHIRLQAAFQKYVDNAVS
                     KTVNFAHSATVADIETVYNLAYDLNCKGVTVYRDGSRDSQVLEVARTEHPAPAAAQDD
                     AAERHLTPRPRKEVTWGSTRKMATGCGALYVTINEDEHGLFEVFATMGKSGGCAASQA
                     EAVSRLVSLSLRCGIEPEQIVKQLKGVRCPNQAWEKGGRIYSCADAIAKAIERYLGHD
                     SRKTEVISVETAKHIAETNGKGSDAVMVGVCPDCHGPLEFESGCSVCRMCGFSRCG"
     gene            complement(1743..2309)
                     /locus_tag="PKJ16_00785"
     CDS             complement(1743..2309)
                     /locus_tag="PKJ16_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45546.1"
                     /translation="MKKVLIVVLAIVAIVVIAILALPGAYTLEEGIEIGAPQPAVFAI
                     AAQPNRWNMLGMMATMGSMPAMPTGQMQLPKEMRDTMPGGMDLGSMMEGMQKIQKTMG
                     SVKIKVKSSAAPGKIVFKIEGGPMDGMEPEMSIERKADRITLVAIRESYTFQGVFSGV
                     KAITAKMASKKLNATNLQNLKRVCEGKK"
     gene            complement(2370..3743)
                     /locus_tag="PKJ16_00790"
     CDS             complement(2370..3743)
                     /locus_tag="PKJ16_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45547.1"
                     /translation="MSARSIISLCAAALALICVSCASFNTRDDYRPTSEALVRGDFDE
                     ALARFPRGERKSFIVTMETACLSLMAGKPDITALEAYSRKIGERVRYKVSRELQSFFY
                     LDTPEGYYASEHEVIWMHLLLSWGYSQRGDFEKARVEVKRAANFIQLETGGPGRFDDP
                     LLRIMCACMWMLCGDWPEAQVDFRAASRLDPKLWWARQLADMKTPPSNLVIVLGGIGP
                     EPAWDPSIGVNLIRGLRGISFNGQGLKSALAATDAKNAPLSLHLSPDSLPWYRRHLAR
                     DNEIQEVIRDTRYAKKTVIASLKAAGIATGSILAGVLVGTGAIALGAGVVYVAVLSGS
                     GEAIGGGIVAGGAIAYAGVDWGVDIGKGGIEKSGDVVRDDLDTSPYYRYVRFLPEYAW
                     VGWSRQPLQFPIEVRRAGSTVYTLNADERRPAATRFFSFGYVPDADVSGLPGRRVRII
                     EYKQPVK"
     gene            complement(3758..4108)
                     /locus_tag="PKJ16_00795"
     CDS             complement(3758..4108)
                     /locus_tag="PKJ16_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45548.1"
                     /translation="MAMELRLEEIDHTLVILHVSGEFYLNDIAEVEEVWRKASGLFPA
                     TIAIDCAGLTAIDSSAIGTMVKFFNSASVKKITLVLYGLGDDLRQLFSRAKLDRFFRI
                     MSSADFEEEFLYYK"
     gene            complement(4108..4890)
                     /locus_tag="PKJ16_00800"
     CDS             complement(4108..4890)
                     /locus_tag="PKJ16_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HNV45549.1"
                     /translation="MGVQLAFLGTGTSNATRKGPPALAFSNGEEVVAVDFGGGAYHQL
                     VRLGSDAFNYKNLSTIIITHFHNDHVSGLPDLFWGEMFDVSGRRETPLTLVGPHGLSN
                     FYQDRLMPFLGGYPIPFEVRLVELANDEQHAGSFYSARAVHLEHVEFSNGYRFSFDGA
                     ELAVTGDTSLCDNLYGLLRGCDAAVLEWALPAPGVFPGHLSNEDMARLIAADALPGRV
                     YVTHVYPVGAGDFEAHVDVRRRILGDAAARFVFPDDLQVFEL"
     gene            complement(4984..5961)
                     /locus_tag="PKJ16_00805"
     CDS             complement(4984..5961)
                     /locus_tag="PKJ16_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV45550.1"
                     /translation="MKKAVSMMAIAFLVAATFGCKKKQPEDVARAGFVNFVNGQVMIV
                     SDKGAENPAKVGDKVSQGMKIKTVGDKSFVDVYFGENAVKVLGNTTVEVSALVTSVKT
                     NGEKTEFMVEKGRMFCKITKKLAKDDSFSVKTPTAVAGVRGTNFLVKSDEKKTNVACV
                     EGLLEVLNKSMPNSDPVVVDKEKEVDVLPDQKMAVRDLSDENKKMIRDILSEISDLRK
                     DIWEQMERQREEIKKHVEEQRARDKEMVQQQKDQDKQNVEDQKARDRAMIGEIKGETD
                     AKRSEAISAAKDKMDAAKNVDLGGAKPAIEKQKIDKSGFKPDMNVKPQQ"
     gene            6219..6587
                     /gene="acpS"
                     /locus_tag="PKJ16_00810"
     CDS             6219..6587
                     /gene="acpS"
                     /locus_tag="PKJ16_00810"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HNV45551.1"
                     /translation="MILGIGTDIVSIERIAELRRRHADRFVRRILSEAEVSIMPGAGA
                     DAFLAGRFAAKEAFVKAIGGRAGIAFADIAILNDDAGKPALVASGRIAAALPDGAART
                     HVSISHEREFATAVVIIETG"
     gene            complement(6673..7947)
                     /gene="glyA"
                     /locus_tag="PKJ16_00815"
     CDS             complement(6673..7947)
                     /gene="glyA"
                     /locus_tag="PKJ16_00815"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HNV45552.1"
                     /translation="MSGKSSNVLLKNSDPEVFDIIRKENERQENTLILIASENYVSQP
                     VLEASGCTMTNKYAEGYPGKRYYNGCQWVDAVETVAIDRIKQLFKADVANVQPHSGTQ
                     ANMAVLLSTLSPGDCFMGMNLSHGGHLSHGSPVNISGIYYKVVSYGVTKDTNRIDFDE
                     AWKNAKEHKPKLIICGASAYPRTIDFAKFREIADSVGAVLLADIAHIAGLVATGDHPS
                     PVPVSDYVTFTTHKTLRGPRGAVILSKLDHEKAVNKFIFPGIQGGPLMHTVAAKAVAF
                     KEALSPGFSAYSKRVIANAQTLAETLQSRGLALVSGGTDNHLMLVDLRNKNLTGRDVA
                     NRLDAAGITCNKNGVPFDDKSPMVTSGIRLGSPALTTRGLGADDFTRIGNLIGDIIDN
                     MNDDAVLARVQGAVAEICAAYPTAFLRLPERV"
     gene            8112..8897
                     /gene="mazG"
                     /locus_tag="PKJ16_00820"
     CDS             8112..8897
                     /gene="mazG"
                     /locus_tag="PKJ16_00820"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HNV45553.1"
                     /translation="MTFKETRPLYLLQEVARILRAENGCPWDREQTHASLKPYLIEEA
                     YEVYDAIESEDPEHLREELGDLMYQVYAHAQIATEAGRFDIDDVARLAADKLIGRHPH
                     VFGDATADTSDAVIRNWELIKKAEKPHRESILDGVPRHLPALLKAYRVQQKVSRVGFD
                     WERIEGIVEKLDEELAEFKEAVALGAQEKMLEEIGDILFTIANIARFIKINPEEALAK
                     TVDKFITRFRFVEADVARSGRALENVPLAELDAAWERAKASER"
     gene            complement(8956..10119)
                     /locus_tag="PKJ16_00825"
     CDS             complement(8956..10119)
                     /locus_tag="PKJ16_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HNV45554.1"
                     /translation="MFTIQTLNSISRCGLDLLPGDRYTHAPALVDPDAILVRSADMHA
                     MELPASVKAVARVGVGVNNIPVDRCSERGVVVFSTPGANANGVKELVIMALIMCSRSI
                     PQAIAWAQGLKGAGAEVPARVEQGKSKFIGPEIKGKRIGVIGLGAIGVMVANDAVALG
                     MQVSGRDPFISADAAGRLSREVKISIDLDSLLAESDYITLHIPQTDETKGFLNRQCFS
                     LMKKGVRILNFSRAGLVNNADLVESIRAGTVATYVTDFPEDELLGVEGVIPIPHLGAS
                     TPESEDNCAIMAVRQLVDFLENGNIVNSANFPDCAMPRSANRRIVIANRNVPNIIGQF
                     ARILAQRTINIGNMLNKSKGDVAYNIIDIDGDIDAAVIGALGAIDGVVMVRAL"
     gene            complement(10138..11220)
                     /gene="serC"
                     /locus_tag="PKJ16_00830"
     CDS             complement(10138..11220)
                     /gene="serC"
                     /locus_tag="PKJ16_00830"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNV45555.1"
                     /translation="MSRVFNFSAGPAVLPESVLRTAASELLEYRDSGMSVMEMSHRSR
                     DYEAIIQDAERLLREIMGIPEQYSVLFLQGGAHLQFAMVPLNLFRGSKKADLVHTGAW
                     TTRAIAEAKKYGTVNIVASSEDANFSYIPKLDTKAFSPDADYFYICSNNTIEGTRFTV
                     WPDTGPVPLVADMSSNILSEVMDVSRFGVIFAGSQKNVAPAGVTIVIIRKDLIGAAME
                     ITPTMLDYATHAKENSLYNTPPTYTIYVAKLVFEWIMEQGGLPAIEKRNVEKANLLYD
                     YLDESTLFKGTVPREDRSLMNIPFVTGSAALDDAFITAAAKNGLVQLKGHRSVGGMRA
                     SIYNAMPVEGVQALVEFMKRFEAENA"
     gene            complement(11407..13113)
                     /locus_tag="PKJ16_00835"
     CDS             complement(11407..13113)
                     /locus_tag="PKJ16_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HNV45556.1"
                     /translation="MASACWKSRGRMIRAALAALCALGLSGAPAHAASVSETELVPSR
                     IAVGDTAALRITVNDVDDAEPVSAPRVAGLDISFNGAQHFSNVQIINMQMKKSAGVVL
                     TFAVSPQRPGTFVIPSVAIRAGSRRLSSRAVTLYASAGQARPRDNLRPGSRAVRLYGS
                     IECSKRAVFVGEPVVLRYFILHSGVEFEQNPMPEKMPETKGFVQKAVEERLEAGAENR
                     DGAELAKTHFATYVLIPTQSGEFTIGGGSAIVVAGSRDEFFGFSFPRQRRVLFREERV
                     SVRALPEAGKPDDFQGNVGSFTIEASSPPGRTPVFEELKLTVKVRGRGNLISLVRPAA
                     IEAGDTIRVIGSDGKETLRIEQGALAGEKEFTFSLIPERSGPFDLGGFTLNFFNPESM
                     RYESVASGRIAFEATGDASRQAGTAIETAKKADGAIDLNYLAIALALLGVAAIVAAVV
                     LWERRRFREIAARDNRTEQPKRVAPAAGRGGIDRTLHDLSGASRRRDAPVFLKTAEIA
                     INELERLAREGALDAVRAANIRSVKERLFSSKYGGGGLADAEMDELYRAIAEIADGIR
                     PS"
     gene            complement(13082..13765)
                     /locus_tag="PKJ16_00840"
     CDS             complement(13082..13765)
                     /locus_tag="PKJ16_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45557.1"
                     /translation="MMPRVLVIALALLTVAWLDPFRDRVSEGNRKFRDKKYEDAKERY
                     RDAARYAPNERARTTLGFNRGAADYMLERHEDAAAAFSQALQAEDRDVQKRAFFNLGN
                     TYLKEKKYREAIDAYVSALRIDPTYEAPKKNLEYLLKKNQQQNKDDQKNGKDGSDGKN
                     DRKDTNDRKKPQDKKDAKDKDGDDRQQRPQGAMSREQVKNILESMKNKPVRRSKGKGD
                     GIRMLEKPW"
     gene            complement(13762..14769)
                     /locus_tag="PKJ16_00845"
     CDS             complement(13762..14769)
                     /locus_tag="PKJ16_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV45558.1"
                     /translation="MHFGNLAYVVYIALACGIVLAFYGLYLFWKNRVMARVAADPRMR
                     RLILRERPIIARVKTALVALAILLAGIVLLRPQWGESAREERKEGVDLLIALDVSMSM
                     MARDVSPSRLARAKDAIRLMADSVKGDRVGLVLFAGDAFLQCPLTNDLGAFSMFLDAA
                     SPDAMRLQGTDLGAAVSEAERVFAKRRRTSRVFALITDGEDHQGALEAAMERFAKLEV
                     TVFTVGIGASSGDYIPLAEADPSGDSYARDAGGNLIRTKKNTETLERIASRTGGEYVD
                     ITESLAGVYRITRAMSRMSANEYGARVVKERNERYQIFALVLAVLLAIEIMLPGRRRE
                     V"
     gene            complement(14769..15767)
                     /locus_tag="PKJ16_00850"
     CDS             complement(14769..15767)
                     /locus_tag="PKJ16_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV45559.1"
                     /translation="MYIPDFKNPLLLLLLLPYAAMVFWYYFRRYHLREAAVAISSRRV
                     MRERQSIRVRTYRYLPALRFLTIFLLIVALARPGRGVHYSSVKHLGIDIMIALDLSDS
                     MMGEDFQPRNRLEVARQVVRDFIARRASDRIGLVVFSGDAYLQCPLTVDHAVLADIID
                     EVDFDTVREDGTAIGDALSLAASRMMDLKGKSKIILLLTDGMNNRGIIDPETAAKACA
                     EMGIRVYAVGIGKDGLVPYPATARFLGGQRFMKNHFDETGLKEIARITGGKFYRAASS
                     GVLWENIRDIDALERTEAEAKVYYEFFDKFQALLVIAVALFFLEILLRSVFYRKVP"
     gene            complement(15799..16851)
                     /locus_tag="PKJ16_00855"
     CDS             complement(15799..16851)
                     /locus_tag="PKJ16_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45560.1"
                     /translation="MTTLRAAIVISSALALAALTGGYAADYPEVRAAANPTETTIGAP
                     VVYTVTIAGKNIAGVTAVLPDKKEFFPKDLPGYVEPKKAAGKRDAAAAKDEDPAALIP
                     VCAVGDAAREDNSDAAMRYQTIRVTVTFYRTGTFRLPEIELRGEDGIKIGYKVPEVTV
                     KPVNEKGEFQEIEPPLELGGNYYRLIALVIALVAAAAAAVFIARAIARRRRRRAPAAT
                     VPPIERFMDDIHTLRTRGYVESGDVEAFCVELSSAFRTFLSGQFRTEAMEMTASEIVR
                     YLETLPGRASIGKYRDDIDRVLNLWDLMKFAEFVPSKEILEGNMESTVALAKKLAMEY
                     RPYGAAHETGDTEAAR"
     gene            complement(16860..17693)
                     /locus_tag="PKJ16_00860"
     CDS             complement(16860..17693)
                     /locus_tag="PKJ16_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HNV45561.1"
                     /translation="MRGDDRSVLRRIKLRTRHKLDTVFVGEYHSAFKGMGLSFNSVRE
                     YQYGDDVRNVDWNVSARMNHLFVKEYIEERELSVVLMIDLSASVEFGSARSKRDVVMD
                     IATLFLYLAQMNNDRISVLLFTDRVEKYLVPRKGRKFVLSVLDELVKYRPAGRRTNIG
                     AAIDFLQRVLKKRSVIIMVSDFLDDDYLLKMKLLRRRHDVIPVVISDPMEKELRLFGL
                     AEFVDLETGEAFLSDTVPEKGTLPFLKEFDSINLSTDAPIELPVLHFFEKRNRTILTR
                     Q"
     gene            complement(17695..18678)
                     /locus_tag="PKJ16_00865"
     CDS             complement(17695..18678)
                     /locus_tag="PKJ16_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV45562.1"
                     /translation="MEYAQIEKLTEKIKAENGFIAQIKQEMAKVIIGQERLLDRIIIS
                     FMAGGHILIEGLPGLAKTLTVRAFARAIDTGFSRIQFTPDLLPADLIGTRIYSPKDLN
                     FFTRKGPIFTNILLADEINRAPAKVQSALLQAMEERQVTIGEETHALPSPFMVLATEN
                     PIEQEGTYPLPEAQLDRFFMKTLVDYPTAAEEREMLSRLGEINVDRITPVVKPKAVAD
                     RRALVDAVYFDEKLVAYVVGIVNETRAPRDEALRRYIEYGASPRASIAIMKAARCIAF
                     MRGRGFATPDDVKEIGADVLRHRVILSYEAEADAKSPDDIVRMVFDSVEVP"
     gene            19026..20939
                     /locus_tag="PKJ16_00870"
     CDS             19026..20939
                     /locus_tag="PKJ16_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV45563.1"
                     /translation="MAGEKLLLTGVFGPYGVSDEYAAGLGMQMELLNNQITREQGVHS
                     PRQSYWTFPLYLLAENLAVSSTVLDFPSWSEFTRELKKGYTHVGINFIVPNVMKARRM
                     AEHIRRHHPAMKIILGGYGTTIPDLETIVPHDAKCRGEGVRWLREYFGEDPAAPLRHP
                     VIIGPAYERIYGFSGKPSGGILMPGVGCENGCKFCVTSHNFNRQYLPLLRTGGDIFTA
                     CRRMETAVGARGFSVMDENFLKNPQRARELLEVMTEHNKPYVFDIFSSAEVIATVGVD
                     FLVRLGVHLVWIGVESKRSSFEKTKGIDLSALIAELRAKGIAVNASAILFQDHHDEQT
                     LREDIDYVIGLESDLVQFMNYTPLPATALTAELSAEGRLNDTPYRFLHGAGGLAFVHP
                     HINDRRKHVRYLREAFRRKFLAHGPGVLSMAQTAIAGYRRAVADHARRMRDGLAWNPA
                     TLRYERTDTPVRDEFMESRIRMMRKIATNIRLVLTAARVYAPNGAARKKAKAVARDFT
                     ETLGPAPLPARLASLALVATAAVEWLRTRAARLAGRESVIYQPPSKRVEYPRAISLTK
                     EHATMRALLDMVPEFVLGAMARRNISVRALARRAGISPALVRGIVAGRRHDVTAHTIE
                     AVLAALGYRIVLE"
     gene            complement(21542..22300)
                     /locus_tag="PKJ16_00875"
     CDS             complement(21542..22300)
                     /locus_tag="PKJ16_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV45564.1"
                     /translation="MNQKYLITAIEDKIALVTINRPDKGNALSPEVLTEIGDIFTDLN
                     QREDVHVIVLTGSSKIFSGGFDLESVQTLRRESNEAFTALFHRAYRSIMFCAQPVIAA
                     IEGAAIAGGFDLTLMCDIRYAAENSKFSQREVLLSLVPMFDPLWRVIGMGPAKEIALT
                     GRIFDAQEALRLGYVTKLFTPGTVLSSVMEIARTMASYDRMCMMEIKRFAHNAMNNSV
                     DGAMKMHEWLFRTYVGSDDNRERIKKLLESLKKK"
     gene            complement(22539..23804)
                     /locus_tag="PKJ16_00880"
     CDS             complement(22539..23804)
                     /locus_tag="PKJ16_00880"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV45565.1"
                     /translation="MKRSIKVYSLCLAVLCAVVAGCRETVPIKEMSLAKADMSRAISV
                     KADKYAPAELQASKEKLFECHQQVKKEDLEKATKAAVESQKKAIEAYNKAVPLLAKDT
                     IEIAEKSLQEADEAAAPLLAKPEYGVAEKLVKDAQDKFENKKYYEAYLGAVEADKEAK
                     NARTIALGKKNVLKDSIDEVRSTLADAKRYDADKYAPEKVKLAEENLGIAQGALDDMK
                     LKKGLSAVEVAKINADEAYLASLKETAKKRISAAELVVENANKSAGASGAKDELAGAN
                     ESIKNAKGLFAEGKYKEAIDAADEAKRLAGIVVASKPAQDPDAAVGKDRPDADKDKDK
                     IADADKDYYIYKVKYRPSRRDCLWRIAAEAYKNPRLWRVIYDANRGKVKNPNLIRPGM
                     KLKIPKNPKAVKAKGPEKTEAPAEEKAAE"
     gene            complement(23885..24223)
                     /locus_tag="PKJ16_00885"
     CDS             complement(23885..24223)
                     /locus_tag="PKJ16_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45566.1"
                     /translation="MDITKRNKGDIVILDITGEIDLYNAPEIKDIISKLIEEKKYNVI
                     INLEKVSYIDSSGIGALISSLSNLKKYQGGLKIINVYASVRKVFELTKLTSFFEIFDA
                     EDVAVDSFNK"
     gene            complement(24361..25500)
                     /gene="tgt"
                     /locus_tag="PKJ16_00890"
     CDS             complement(24361..25500)
                     /gene="tgt"
                     /locus_tag="PKJ16_00890"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV45567.1"
                     /translation="MEFKVHATDTATGARTGTLSTAHGEIATPVFMPVATRGAVRALT
                     NRDIDELGFDMILANTYHLYLRPGADALARAGGLHRFMRFDRAILTDSGGFQVFSLSN
                     LCTVREEGVEFRSHIDGSRHLFTPRLVLDAQRAIGSDIMMVLDECIHYPATEAQARGA
                     LEKTVAWARDSHGYWRERFDTGRQALFAITQGGVYEHLRRECIERLLELDVSGYAIGG
                     LSVGEPKELYRAMTELTLGLLPAHKPRYMMGVGSPLEILYAVSRGVDMFDCVMPTRIA
                     RNGTLYTDAGRINIKTARFADDDGPIDAGCPCYVCRTFSRAYLRHIFRAGEIAALIYN
                     TYHNLSFMKRLMDRIAASIAGGSFGALMREYERAYAPGGGDTGGD"
     gene            25570..25643
                     /locus_tag="PKJ16_00895"
     tRNA            25570..25643
                     /locus_tag="PKJ16_00895"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25604..25606,aa:Asp,seq:gtc)
     gene            complement(25668..26126)
                     /locus_tag="PKJ16_00900"
     CDS             complement(25668..26126)
                     /locus_tag="PKJ16_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45568.1"
                     /translation="MRRSRGALFSVVMIAAVVARCASGPRAPQTGWAGDDIYRVAVEA
                     PAVARDGASAGSAAPVSRRATLERAAELARERVLRDFADQRAKALGASGAGQRAWLQL
                     TFDRDIRDAIARGTIVYRSFRDDRSCAVVFQVRREGLRGRVEGENAKEAR"
     gene            complement(26133..27143)
                     /gene="moaA"
                     /locus_tag="PKJ16_00905"
     CDS             complement(26133..27143)
                     /gene="moaA"
                     /locus_tag="PKJ16_00905"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HNV45569.1"
                     /translation="MILRDPYNRVLDYLRISVTDKCNLRCRYCIPPDGVEHLTHDEVL
                     RNEEFLHFIELFAELGVRKIRFTGGEPLVRKGIIDIIAGARAAAPGIELCLTTNGVLL
                     DGALAELMRLGVRKLNISLDTMSSARYREITGRDYFDRVLANIERAIALGAFDLKINA
                     VLFEDSLHEVNDFLEFFHDKDVMLRFIEQMPFTDDRAESGFVPADSLVERLAALGRLA
                     RNDRIDTSVAMMYDLEYRGGRAMRIGVIPPITHTFCARCNRMRLTCDGMLKTCLHGTA
                     EYDLKGPYRMDMGDDALRTIIRRAVAEKPCAHTLVCDSNTGQVCRSLEGAPVRPMSKI
                     GG"
     gene            complement(27256..28704)
                     /gene="gatA"
                     /locus_tag="PKJ16_00910"
     CDS             complement(27256..28704)
                     /gene="gatA"
                     /locus_tag="PKJ16_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HNV45570.1"
                     /translation="MLHRNGLRELADLLTGRQASAREIAQDLLSRVGALNGRLNAFIT
                     VDQERCLREADEADARRARGEARSDFDGIPVAVKDNICTAGLRTTCASGILRDFVPPY
                     DAHAYAGMRAHGFATLGKTNMDEFAMGSTTETSFFGPARNPHDDTRVPGGSSGGSAVA
                     VAALMAPAALGSDTGGSIRQPAAFCGCVGVKPTYGRVSRYGLVAFASSLDQIGAFARS
                     VDDAAALLAAVSGHDRRDSTSIDRPVDFGPDSLTGDVRGLRIGMPEEYFAGVAPEIAD
                     RVRAQIAALEAEGATVRPISLRLTEYAVPVYYLIATAEASSNLARYDGIRYGTPAPDA
                     ANLAELYERTRSAGFGKEVKRRIMLGTFSLSSGYYDAYYLKALKGRTLIIEDFRRAFA
                     EVDVIVTPVTTTTAFGIGEKISDPLSLYLSDILTISANLAAIPGVSVPVGLDARGLPI
                     GLQIMASHFQERVLLKAAKAVERVSPPVAPAL"
     gene            28853..30844
                     /locus_tag="PKJ16_00915"
     CDS             28853..30844
                     /locus_tag="PKJ16_00915"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HNV45571.1"
                     /translation="MSNRKLKITFSIVVTLVLIFSCSRKTTGLKKSDVQPVISLFLNK
                     HVQFRELNDELSRRTFNNILRQLDPGKQFFYKSDIAALSVYENKIDDFIEKEKYDFLS
                     EIYSVYRKRFAANIVLIHELLKGQFDFTIDESVTIDPDAIDYVSTDAEMRERWRKSIK
                     LQLLNYLDVINDMGKAKEKIVKRYQLFERRVNEVDEAKMMDAFANAFSTALDPHSNYL
                     SPEDHEDFMIQTKLKLEGIGVMLRSEDGFVTVESIVPTGAAAKLPVDLQLKLGDKIIG
                     VSQGDGEMQDVIDRDLRDVVRLIRGKKNTEVRLKVVRRLDEKNKQQQLIIPIKREEIK
                     LETSSAKSEVYVSPRPAASAKIGYIRLPTFYFDFDAAQSFDKNTKSSFHDFRAQLASL
                     KKTGVDGIVIDLRGNPGGALNEAVYLTGLFIDTGPVVQVKDGNNNISIMRDDERGMTY
                     DGPIVVLIDGFSASASEIFAGAIRDYRRGLILGPTKTFGKGSVQNYLKLADGAKGAVK
                     ITTALFYQPVGTSNDLIGINPDIIVPDISAVWDIGEHKLKYTIKWEKIPSAPYKPYTK
                     YLNGTIVSQLISESNKRVSSSKKFADLIQRIKKLKAKVNAKSISLKKEDFSSEEKDLK
                     EIKKKMRDENDKKLIDLERDLFLQEAFDITTDYIRMLKR"
     gene            complement(30834..31970)
                     /locus_tag="PKJ16_00920"
     CDS             complement(30834..31970)
                     /locus_tag="PKJ16_00920"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV45572.1"
                     /translation="MQQSSLGFMVDPLRALLEQWDGCMTALRTGEGKQVVGAVCGFLP
                     MEVLAAAGTIPVRIPARILAGAHAVSGACGTEGDAACVAPGCCDAMVVSASCCRCARN
                     SPQDARVIVFESPSGYGEGAAVKLHESLRDCLQRLGCPDAAALDPDALAAAVEERNAV
                     RRLVRGICAERRDRPAALSQEDLLAIMEYAACFPPAHAIERLSPIRDALNDFPAGNDG
                     AAALPALVHGGFFFDWRVFDEIEEAGCRVDEDDLCNGRRQFDISYNAESPHLYAEILD
                     AFSYRPLCPSIRPLGERVDLLYRLLRTHGIETVVFVGSGCCVARDRERTGMSRTLMRL
                     GIDPVAAGGEDPAAAASYHMKRMRDLPVIECGEAATGGGEPTTV"
     gene            complement(32141..33772)
                     /gene="groL"
                     /locus_tag="PKJ16_00925"
     CDS             complement(32141..33772)
                     /gene="groL"
                     /locus_tag="PKJ16_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256012.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HNV45573.1"
                     /translation="MAKILQYNEEARRSIMTGVNKLNDAVKVTLGPRGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEEPFENMGAQLVKEVATKTNDVAGDGTTTATLLATAIYRES
                     LKNVTAGSNPVNLKRGIDQAVKAAVDAVKNLSREIKDKKEIAQVAAISANNDNEIGEL
                     IAEAMEKVGKDGVITVEEAKSIDTHLEVVEGMQFDRGYISPYMVNNPDTMEAVFENAY
                     ILINEKKISTMKDLLPVLEKVAQAGKPLVIISEEVEGEALATIVVNTLRKTISCVAVK
                     APGFGDRRKAMLEDIAILTGGQVISEDLGLKLENTTVEMLGKAKKIIVDKENTTIIEG
                     GGKAKEVQARISVIKKQIEETTSDYDKEKLQERLAKLAGGVAVINVGAASEVELKEKK
                     ARVEDALSATRSAVEEGIVPGGGMALLYAQKSVIELVGKLQGDEKIGAEIIVRAVEEP
                     MKTIASNAGVEGSVIVERAKGEKTNIGFNASTLVWEDLVKAGIIDPAKVVRTALQNAS
                     SVAGILSTTEVLITDKKEDEKAPAMPGGMPPGGMY"
     gene            complement(33793..34077)
                     /gene="groES"
                     /locus_tag="PKJ16_00930"
     CDS             complement(33793..34077)
                     /gene="groES"
                     /locus_tag="PKJ16_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HNV45574.1"
                     /translation="MAIKPLGDRVLLEVIDDDLQKQGSIYIPDTAKEKPQQGKVVSVG
                     NGRYEDGKLIPLDVKAGDTVLFGKYSGTEVKYQGKEFLIVRENDILAVID"
     gene            complement(34344..34820)
                     /gene="moaC"
                     /locus_tag="PKJ16_00935"
     CDS             complement(34344..34820)
                     /gene="moaC"
                     /locus_tag="PKJ16_00935"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HNV45575.1"
                     /translation="MSDFSHYEKDGRSVMVDVSAKRETSRLARASGFVSMSAETIDAI
                     ENRLLPKGDPFEVARVAGILAAKGASGLIPMCHPLMITFVDVVIRIDRPAGGVVIESE
                     IRLNGKTGAEMEALMAVSVAALTVYDMCKAVDASMVLGNIHLVEKRGGKSDITRDI"
     gene            complement(34894..35634)
                     /locus_tag="PKJ16_00940"
     CDS             complement(34894..35634)
                     /locus_tag="PKJ16_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNV45576.1"
                     /translation="MNLAWVPNSLTLGNLLLGFMSIVIASHGGAPAATIAGALILGAA
                     LLDGFDGQAARALKVTSPLGAELDSLADCVAFGVAPGYLAYKAYLSGIIVSGIAVPYI
                     NIPFDFGIFIAALFPICAAYRLARFNVNSDSKSFTGLPSPIAGIIVAVVPIFIGDVIL
                     ADTIYRVIFAVLFSLVALLMVSTVRYSKPQATFFQRYIFVKIVLLLAIFALLVLLFRE
                     WSVFIVLALYILSGLVSFIIQFIQDHKY"
     gene            complement(35740..36417)
                     /locus_tag="PKJ16_00945"
     CDS             complement(35740..36417)
                     /locus_tag="PKJ16_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNV45577.1"
                     /translation="MSARAFPERCRAVLFDMDGVIVDSMPYHADAWMGAIRECCGLEL
                     SREDIFRREGMPGITSIIDILSEKNAPIPDEAELVRIREAKLRIFEHAVVRVFPGVAD
                     ILAMLAKRNIARGLVTGSMRRSVRHLLPPTLRTAFQAIVTGDDIRNGKPHPEPYLSCL
                     ARLGVDAAQAIAVENAPMGIRSARGAGLACVAIRTTLDETHLSEADAIVPDHDALRTF
                     LEKALGA"
     gene            complement(36445..37545)
                     /locus_tag="PKJ16_00950"
     CDS             complement(36445..37545)
                     /locus_tag="PKJ16_00950"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HNV45578.1"
                     /translation="MITVGVLFGGRTGEHDVSLCSAASVVSALDASRYAVVAIGIDRA
                     GRWYVQDAPGVVPDPEFGRKMRLERTGTWLAANHPDDNRLTLREIGSGQEVRLDVVFP
                     VMHGTYGEDGTLQGLLELAMVPYVGADVIGSAIGMDKDVSKRLLAQAGIPVTPGVTVS
                     RRDWERRKREMVDEAIGRLGLPLFVKPSCAGSSVGVRKAKIEAELCDAVDFAMQFDTK
                     VLVERSVPCREIECAVLGNDEPAASVLGEIVPNHEFYSYEAKYLDPDGARLSIPADVP
                     SALAEEIRSCACRAFTVLCCAGLARVDFFLDRETGAYYLNEINTLPGFTSISMYPKLW
                     EASGLPYAALLDRLIDLAFERHAVRAALKTEC"
     gene            complement(37592..38809)
                     /locus_tag="PKJ16_00955"
     CDS             complement(37592..38809)
                     /locus_tag="PKJ16_00955"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HNV45579.1"
                     /translation="MKIIVQKYGGTSVGTTERIKAVAQRIKGYIDNGYSVVVIVSAMG
                     KSTDELVRLAGEITAQPSKREMDMLLSTGEQISIALLAMALHEAGLTAVSYTGSQIRM
                     VTDGNFTNAKIHSISSEKIHDALRSNNVVIVAGFQGVDRDDNITTLGRGGSDTSAVAI
                     AAALGVRDCEIYTDVDGVYTADPRIVARTRKLREISYEEMLELARLGAGVLHSRSVEF
                     AKKYDVRLHVRSSFSSEEGTIVTSREEMMEKFVISGVTAKSDEAKVTIRDIVDRPGVA
                     SKLFGCLGENKIYVNMIVQSTGHDGKASISFTVKKDDLARAKELCGGLKGDLGGTSVE
                     SKEDIAIVSAVGVGMLSSYGVAGRMFEVLSRENVNIEMISTSEIGISCVIDSKYAELA
                     VKIIHKAFLEENQ"
     gene            complement(38928..>40004)
                     /locus_tag="PKJ16_00960"
     CDS             complement(38928..>40004)
                     /locus_tag="PKJ16_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45580.1"
                     /translation="VYTLDLNDSEPFFSGMGIGHPNDDVEAGRLWTGMRLFLRKMVFD
                     GAMQYHLIPGEPGIGTWTSDGKLYTIFDVDSVEGLDVNPLQVNTYYDSLKQNAKEINR
                     IFPLKIPIIGGLNFNYANDETVLEIRLVFKNFVKRYEYDFVRDGYHYIYRYFGPSDWL
                     RDVVADDAVIGGNIIAVARSYVRGKTVDITNAHTTGITVVANSYVAAVGPDSLCASLN
                     RPSVVRPTLRTSGTPPAQCDNPKGPHVPIPNDIESYLDYYLKYEKYKENYNLFAACVS
                     DGSFSTLWDEYNDSRNAYLLPAVATWTSDGSFTLKNVPYNSSWKICHRQTSPGAGDLP
                     EFTDNAGDFVVVTIDGGGNVAISP"
BASE COUNT         7372 a        12986 c        12057 g         7589 t
ORIGIN      
        1 caacttcaat ctctcggtcg ccatcaccga cgagttcatg cgcgcggtcc gcgagaaccg
       61 cgactacgag ctcgtaagtc cccgtacggg ccggccggtc gccgtgcgca acgcccgcgc
      121 ggtgtacgcc gccatcgtgg agcacgcctg gcacagcggc gagccgggca tcgtcttcat
      181 cgaccgtatc aacgccgcga accccttgaa gcgggtgggc gacatcgaaa gcaccaatcc
      241 ctgcggcgag cagccgctgc tgccgttcga atcgtgcaac ctgggctcca tcaacctgag
      301 ccgcgtgctg acgagcaccg gcggcgccta cgagatcgac ttcccgcggc tcggcgagat
      361 cacgcgcgcg gcggtccact tcctcgacaa tgtgatcgac cgcaaccgct acccgctccc
      421 cgagatcgcc gagagcacgc ttgcgaaccg gaagatcggc ctgggggtga tgggcttcgc
      481 cgatacgctc atcatgctgg gtattccgta cgactccgag gaggcgatcg aaaccgccga
      541 gcgcatcatg tcgttcatcc aggacgagtc gaaggcggcc tcgcgggagc tcgcgcgcga
      601 gcgcggggcg ttcccgaact tcgagcgctc atcgtacgcc gaggcgggcg agccgccgct
      661 tcgcaacgcg accaccacga ccatcgcgcc cacgggcacc atcagcatca tcgccaattg
      721 ctccagcggc atcgagccga tcttcgcgct cgcctatgtg cgcaatgtca tggacaacaa
      781 caccctcgtc gagacgaatc ccttcttccg ttcatacgcc gatgcgaacg gcttctacag
      841 cgacgagctg atgacggaca tcgcgcggca cggctccgtg cgcgacatcg acaccgtccc
      901 cgaggcggcg cgccggctct tcgttaccgc gcacgacatc acgccggaat ggcacatccg
      961 cctccaggcc gccttccaga agtatgtcga taacgccgtc tcgaagacgg tgaactttgc
     1021 ccatagcgcg acggtagccg atatcgaaac cgtgtacaac ctcgcctacg acctgaattg
     1081 caagggcgtg accgtgtacc gggacggctc gcgcgattcc caggtgctcg aggtcgcgcg
     1141 caccgagcac cctgcgcccg cggcggcgca ggacgacgca gcggagcgcc acctcacgcc
     1201 ccgcccccgc aaggaggtca cctggggatc cacgcgcaag atggcgaccg gctgcggcgc
     1261 gctctacgtg accatcaacg aagacgagca cgggttgttc gaggtgttcg ctacgatggg
     1321 caagagcgga ggatgcgcgg ccagccaggc cgaggcggtg agccgcctgg tgtcgttgtc
     1381 gctgcgctgc gggatcgaac cggaacagat cgtgaagcag ctcaaagggg tgcgctgtcc
     1441 caaccaggcc tgggaaaagg gcggccgcat ctactcatgc gcagacgcca tcgccaaggc
     1501 catcgagcgc tatctgggcc acgactcgcg caagacggag gtcatctccg tcgagacggc
     1561 aaagcacatc gccgagacaa acggcaaggg aagcgacgcg gtgatggtgg gcgtgtgccc
     1621 ggactgccac gggccgctcg agttcgagtc gggctgctcg gtgtgccgca tgtgcggctt
     1681 ctcgcggtgc gggtaggatt tcattgagac agggcggcgc cctgtctcaa tcaacggtat
     1741 cgctattttt tcccctcgca cacccgtttg aggttttgca ggttcgtcgc gttgagcttt
     1801 ttcgacgcca tcttcgcggt gatggccttg acgccggaaa acaccccctg gaatgtgtag
     1861 ctctctcgaa ttgcgaccaa cgtgatcctg tcggccttcc tctcgatgct catctccggc
     1921 tccatgccgt ccatggggcc cccctcgatc ttgaaaacga tcttcccggg ggccgcgctg
     1981 cttttcacct ttatctttac cgatcccatc gtcttctgta tcttttgcat gccctccatc
     2041 atggacccaa gatccatgcc gccgggcatc gtgtcccgca tctccttggg gagctgcatc
     2101 tgccccgtgg gcatggcggg catcgatccc atcgtagcca tcatgccgag catgttccaa
     2161 cggttcggct gcgccgcaat cgcaaagacg gccggctgcg gagcgccgat ctcgatgccc
     2221 tcctcgagcg tataggcgcc cggaagcgcg agtatcgcga tcacgacaat cgcgacgatg
     2281 gcgagaacga caatcaggac ctttttcatg cgaacctccc gtgatctgcg tgttgatcgc
     2341 gcgcccgaaa tcccaaaccc gcggcgcgat tatttgaccg gctgtttata ttcgatgatc
     2401 cggacccgcc gtcccggcag gccgctcacg tccgcatcgg gcacgtaccc gaacgagaag
     2461 aatcgggtcg ccgcgggccg ccgctcgtcg gcgttcagcg tgtacaccgt cgatccggcg
     2521 cgccgcactt cgatcgggaa ttgcagcggc tggcggctcc accccaccca ggcgtactcg
     2581 ggaagaaagc gcacgtaccg gtaatacggc gaggtgtcga ggtcatcgcg cacaacgtcg
     2641 ccgctctttt cgatgccgcc ctttccgatg tccacgcccc aatcgacccc cgcataggcg
     2701 atggcgcccc ccgcgacgat cccgccgccg atcgcctccc ccgatcccga gaggacggcc
     2761 acgtagacga cgccggcacc cagcgcgatc gcgcccgtac cgacgaggac gcccgccagg
     2821 atgctccccg tcgcgatccc cgcggccttg agcgacgcga tgaccgtttt cttcgcatac
     2881 cgcgtgtcgc ggatcacctc ctgtatctca ttgtcgcgcg ccagatggcg ccggtaccag
     2941 ggaagcgaat cgggcgagag gtggagcgag aggggcgcat tcttcgcatc cgtcgcggcc
     3001 agcgcgctct tgagcccctg cccgttgaag gaaatgccgc ggagtccccg gatgaggttt
     3061 acgccgatgg acgggtccca ggccggttcg gggccgatgc cgcccaggac gatcaccaga
     3121 ttggacggcg gcgttttcat gtcggcaagc tgccgcgccc accagagttt tgggtccaac
     3181 ctcgatgccg cccggaaatc gacctgcgcc tcgggccagt cgccgcagag catccacatg
     3241 cacgcgcaca tgatgcgcaa gagcggatcg tcgaaccgcc ccggccctcc cgtctcaagc
     3301 tggatgaagt tcgccgcgcg cttcacctcg acccgcgcct tctcgaaatc gccgcgctgg
     3361 gaatatcccc acgagagaag caggtgcatc cagatcacct cgtgctcgga ggcgtaatag
     3421 ccctcgggcg tatcaagata gaagaacgac tgcagctcgc gcgacacctt gtagcgcacg
     3481 cgctcgccga ttttacgcga atacgcctca agcgcggtga tgtcgggttt gccggccatc
     3541 aggctcaggc aggccgtttc catcgtgacg atgaatgatt ttctctcgcc gcggggaaag
     3601 cgcgcgagcg cctcgtcgaa atcgccgcgc acgagcgcct ccgacgttgg acgatagtcg
     3661 tcgcgggtgt taaacgaggc gcaggagacg catatcagcg ccagcgcggc cgcgcacagc
     3721 gaaatgatcg atcgggcgga catcatggct ccggacatta cttatagtac aggaactcct
     3781 cttcgaagtc cgcgctcgac atgatacgaa aaaaccggtc cagtttcgcg cggctgaaaa
     3841 gctgccggag atcgtcgcca agcccgtaga gcaccagcgt gatcttcttg acgctggcgc
     3901 tgttgaagaa cttgaccatg gtcccgatgg ccgaggagtc gatggcggtg aggccggcgc
     3961 agtcgatggc gatggtcgcc ggaaagagcc ccgacgcctt ccgccacacc tcctccacct
     4021 ccgcgatatc gttgagatag aattcacccg acacgtgcag aatcaccagg gtgtggtcga
     4081 tttcctcgag gcgaagttcc atggccatca gagctcgaac acctgaagat cgtccgggaa
     4141 cacgaagcgc gcggccgcat cgccgaggat ccggcgtcgc acatcgacat gggcctcaaa
     4201 atcgcccgcg ccaacgggat agacgtgcgt aacatagacg cgcccgggaa gcgcgtcggc
     4261 cgcgatcagg cgcgccatgt cctcatttga cagatgcccc gggaacaccc cgggggccgg
     4321 gagcgcccac tccaggacgg ccgcatcgca gccgcgcagc aggccgtaga gattgtcgca
     4381 caggctcgtg tcacccgtaa ccgccagctc ggcgccatcg aaggaaaagc ggtagccgtt
     4441 ggaaaactcg acatgctcca gatgcaccgc gcgggcggaa taaaagcttc ccgcgtgctg
     4501 ctcgtcgttc gccaactcca ccaggcgcac ctcgaagggg atggggtatc ccccgaggaa
     4561 gggcatgagc cggtcctggt agaaattcga aagcccgtgc ggccccacca gggtgagcgg
     4621 cgtttcgcgg cggccgctca catcgaacat ctcgccccag aagagatcgg ggagcccgct
     4681 cacatgatcg ttgtgaaaat gggtgatgat gatggtcgag agatttttat aattgaatgc
     4741 gtccgagccc aggcgcacga gctgatggta ggcgccgccc ccgaaatcga cggcgaccac
     4801 ctcctcgccg ttcgaaaacg caagcgccgg cggccccttg cgggtcgcat tggacgtgcc
     4861 ggtccccaaa aacgccagtt gaactcccat gtcgcctccc cccggaaacg caggctccct
     4921 caccgaacga tgagggagcc tggtgcgcgc ggtcttacga tctagaacgt cgcacgagca
     4981 gccttactgc tgcggcttga cgttcatgtc gggcttgaag ccgctcttgt cgatcttctg
     5041 cttttcgatg gccggcttgg cgccgcccag atccacgttc ttcgcggcgt ccatcttgtc
     5101 cttcgcggcg ctgatggcct cgctgcgctt cgcgtcggtc tcgcccttga tctcgccgat
     5161 catggcgcgg tcgcgcgcct tctggtcctc gacgttctgc ttgtcctggt ccttctgctg
     5221 ctggaccatc tccttgtcgc gggcgcgctg ctcctcgaca tgcttcttga tctcctcacg
     5281 ctggcgctcc atctgctccc agatgtcctt gcgcaggtcg ctgatctccg agaggatgtc
     5341 gcggatcatc ttcttgttct cgtcggagag gtcccgcacc gccatcttct ggtcgggcag
     5401 cacatcgacc tctttctcct tgtccacgac caccgggtcc gaattgggca tggacttgtt
     5461 gagcacctcg agcagcccct cgacgcaggc gacgttcgtt ttcttctcgt cgctcttgac
     5521 caggaagttg gtcccgcgga caccggcgac cgcggtcggg gtcttcaccg agaagctgtc
     5581 gtccttggcg agcttcttgg taatcttgca aaacatgcgg cccttctcga ccatgaactc
     5641 ggtcttctcg ccgttggtct tcacgctggt gaccagcgcg ctcacctcga cggtcgtgtt
     5701 gcccagcacc ttcacggcgt tctcgccgaa gtagacgtcg acgaacgact tgtcgccaac
     5761 cgtcttgatc ttcatgccct gcgagacctt gtcaccgacc ttggcggggt tctctgcgcc
     5821 cttgtcggaa acgatcatca cctggccgtt cacgaaattg acgaacccgg cgcgggcgac
     5881 gtcctcgggt tgttttttct tgcagccaaa cgtcgcggcg acaagaaacg ctattgccat
     5941 catcgatact gcttttttca tacgaccctc ctcgttgtgg ttcttgctgg cgcagcacgc
     6001 atgcgtgtat tggagaatgc gggcccgtgt tccagatgtt agaatatcac gttactgccg
     6061 gtgagaaagc tatacccgtt ttgacgccga gtcaagcaga aattgtcata atttttccac
     6121 aacaagcctg tccgggcggc aataaatcac ttgcatttgc ccgcaggagg cgcatcctcg
     6181 agacagtgcg cattgcgcac agggtagtct ggagcgccat gattcttggc atcggaaccg
     6241 acatcgtgag catcgagcgc atcgccgagc tgcgccggcg gcatgccgac aggttcgtcc
     6301 ggaggatact gtccgaggcc gaggtttcca tcatgcccgg ggcgggcgcc gacgcatttc
     6361 ttgcgggccg gtttgccgcc aaggaggcgt tcgtcaaggc gattggcggg cgcgcgggca
     6421 tcgcctttgc ggacatcgcg atactcaacg acgacgcggg gaaaccggcg ctcgtcgcga
     6481 gcggccggat cgccgccgcg ctgcccgacg gagcggcccg cacgcatgtg agcatcagcc
     6541 atgagcgcga attcgcgacc gcggtcgtta tcatcgaaac cggatgagtg cggaccgtac
     6601 gtccgctccc cgcagccacc gggcgccggc ggcgcccggc atcagcacgc aacgccgtac
     6661 tcgtttcgcg ggctagacgc gttccggaag ccgcaggaag gccgtcggat aggcggcgca
     6721 gatctcggcc accgccccct gcacccgcgc gaggaccgcg tcgtcgttca tgttgtcgat
     6781 gatgtcgccg atgaggttcc cgatccgcgt gaagtcgtcg gcccccaggc cgcgcgtggt
     6841 gagcgccggg ctcccgagcc ggatcccgct ggtgaccatg ggcgacttgt cgtcgaaggg
     6901 gacgccgttc ttgttgcagg tgatcccggc ggcgtcgagt cggttggcga cgtcccgccc
     6961 cgtgaggttc ttgttccgga ggtccaccag catgagatgg ttgtcggtgc cgccggagac
     7021 gagggcgagg ccccgcgact ggagcgtttc ggccaacgtc tgcgcgttcg cgatcacccg
     7081 cttcgagtac gcggagaagc cgggcgagag cgcctccttg aaggcgacgg ccttcgccgc
     7141 gacggtgtgc atgagcgggc cgccctggat gccggggaag atgaacttgt tcacggcctt
     7201 ctcgtgatcg agcttcgaga ggatcaccgc gccgcgcggg ccgcgcaggg tcttgtgcgt
     7261 ggtgaaggtc acgtagtcgg acaccggcac cggcgagggg tggtcgcccg tggcgacgag
     7321 ccccgcgatg tgcgcgatgt cggccaggag aacggcgccc acgctgtcgg cgatctcgcg
     7381 gaacttcgcg aagtcgatgg tgcgcggata cgcggaggcc ccgcagatga tgagcttggg
     7441 cttgtgctcc ttggcgttct tccacgcctc gtcgaaatcg atgcggttcg tatctttcgt
     7501 gacgccgtag gagacgacct tgtagtagat gccggagatg ttcaccggcg agccgtgcga
     7561 gagatggccg ccgtgcgaca ggttcatccc catgaagcag tcgccgggcg agagcgtcga
     7621 aaggagcacc gccatgttcg cctgcgtccc gctgtgcggc tgcacgttgg ccacatcggc
     7681 cttgaagagc tgcttgatgc ggtcgatggc gaccgtctcc accgcgtcca cccactggca
     7741 cccgttatag tagcgtttgc cgggatagcc ctccgcgtac ttgttggtca tggtgcaccc
     7801 ggaggcctcg agcaccggct gcgagacata gttctccgag gcgatgagga tgagcgtgtt
     7861 ctcctggcgc tcgttctcct tccggatgat gtcgaatact tcgggatcgg agttcttcaa
     7921 taggacgttc gatgactttc cgctcatggc gccgctcctc cgcgcaaaat aaatgaaggt
     7981 catgctatcg acgggggagg gaaacctgtc aagataaaat cgtgcggcct tccgtaccgc
     8041 cgcccgcagg cggtttattt catttacaaa atcacgcgca tcacgacaat gcccctgcga
     8101 tggagggtcc aatgacattc aaggaaacac gccccctgta cctgcttcaa gaagtcgccc
     8161 gcatcctgcg cgccgagaac ggctgcccgt gggaccgcga gcagacgcac gcatcgctca
     8221 agccctacct catcgaggag gcctacgagg tctacgacgc catcgagtcg gaggacccgg
     8281 agcacctcag ggaggagctc ggggacctga tgtaccaggt gtacgcgcac gcgcagatcg
     8341 cgacggaggc ggggcgtttc gatatcgacg acgtcgcgcg cctggccgcc gacaagctca
     8401 tcgggcggca cccgcacgtc ttcggcgacg cgacggccga caccagcgat gccgttattc
     8461 gaaactggga gctcatcaag aaggccgaaa agccgcaccg ggagtccatc ctcgacggcg
     8521 tcccgcgcca tcttccggcg ctcctgaagg cctatcgcgt gcaacagaag gtctcgcgcg
     8581 tgggcttcga ctgggagcgc atcgagggga tcgtggaaaa gctcgacgag gagctcgcgg
     8641 agttcaagga ggcggtggcg ctgggcgcac aggaaaaaat gctggaggag atcggcgaca
     8701 tcctcttcac catcgcgaac atcgcgcgct ttatcaagat caaccccgag gaggcgctcg
     8761 cgaaaaccgt ggacaaattc atcacgcgct tccggttcgt ggaagccgat gtcgcgcgct
     8821 ccgggcgcgc gttggagaac gtgcccctcg ccgaactcga cgccgcgtgg gagcgcgcca
     8881 aggcgtccga gcgctgaacg aaccgggcgc ggcgacgcac gaatcatcct cgcgccgccg
     8941 tcccgaagaa cacccctaca gcgcgcgcac catgaccacc ccgtcgatcg cccccagtgc
     9001 gccgatgacg gcagcatcga tatcgccgtc gatgtcgatg atgttgtagg cgacgtcccc
     9061 cttgctcttg ttgagcatgt ttccgatgtt gatagtgcgc tgcgccagga tgcgcgcgaa
     9121 ctggccgatg atattgggca cgttccggtt tgcgataacg atgcgccggt tcgcggagcg
     9181 cggcatcgcg cagtcgggga agttcgccga gttgacgatg ttcccgttct ccagaaaatc
     9241 gacgagctgc cgcacggcca tgatcgcgca attgtcctcg gattcaggcg tcgaggcgcc
     9301 caggtgcggg atcgggatca ccccctcgac gcccaggagc tcgtcctcgg ggaagtccgt
     9361 cacgtacgtc gcgacagtcc ccgcccggat cgactcgacg agatcggcgt tgttcaccag
     9421 ccccgctcgc gagaaattga gaatgcgcac gcccttcttc atgagggaga agcactgcct
     9481 gttgaggaac cccttcgttt cgtcggtctg cgggatgtgc agcgtgatat aatcggactc
     9541 cgcgagcagg ctgtcgaggt cgatggaaat ttttacctcg cgcgacagcc tcccggccgc
     9601 gtccgccgag atgaacggat cgcgcccgga tacctgcata cccagcgcca cggcgtcatt
     9661 ggccaccatc acgccgatcg cgccgagacc gatcacgccg atccgtttac ccttgatctc
     9721 gggaccgatg aatttcgact tcccctgttc cacgcgggcc ggcacctcgg cgcccgcccc
     9781 cttgagcccc tgcgcccacg caatcgcctg cgggattgag cgcgagcaca tgatgagcgc
     9841 catgatgacg agctccttga cgccgttggc attggcgccc ggggtgctga acaccacgac
     9901 gccccgttcg gagcacctgt ccaccggtat gttgttcaca ccgaccccga cgcgcgcgac
     9961 ggccttgacc gacgccggca gctccatcgc gtgcatgtcg gcgctgcgca ccaggatcgc
    10021 gtccggatcg acgagcgccg gggcgtgcgt ataacggtcg ccgggcaaca ggtccaggcc
    10081 gcagcgggag atgctgttga gcgtttggat tgtaaacatc gtgcagcgct ccttgttcta
    10141 cgcgttctcc gcttcgaacc gcttcatgaa ctcgacaagc gcctgtacgc cctcgacggg
    10201 catggcgttg tagatgctcg cccgcattcc gcccacggac cggtgcccct tgagctgcac
    10261 caggccgttc ttcgcggcgg ccgtgatgaa ggcgtcgtcg agcgccgcgc tgcccgtgac
    10321 aaagggaatg ttcatgagcg agcggtcctc ccgggggacc gtccccttaa acagcgttga
    10381 ttcgtcgagg tagtcgtaca gcaggttcgc cttctcgacg ttgcgcttct cgatcgcggg
    10441 gagcccgccc tgctccatga tccactcaaa gacgagcttc gccacgtaga tggtgtaggt
    10501 gggcggcgtg ttatagagcg agttctcctt cgcgtgcgtg gcgtaatcga gcatggtagg
    10561 ggtgatctcc atcgccgcgc cgatgaggtc cttccggatg atcacgatgg tcacgcccgc
    10621 gggggcgacg ttcttctgcg agccggcgaa gatgacgccg aacctcgaga cgtccatcac
    10681 ctcggagagg atattggatg acatgtccgc gaccagcggc accgggccgg tgtcgggcca
    10741 cacggtgaag cgggtgccct cgatggtatt gttcgagcag atgtagaagt aatcggcgtc
    10801 gggcgagaag gccttcgtgt cgagcttcgg gatgtagctg aagttcgcat cctcggagct
    10861 ggcgacgatg ttcaccgtcc cgtacttctt cgcctcggcg atggcccgcg tggtccacgc
    10921 gccggtgtgc accaggtcgg ccttcttgct gccgcgaaac agattgagcg gcaccatcgc
    10981 gaactgcagg tgcgcgcccc cctgcaggaa gagcaccgaa tactgttccg ggatgcccat
    11041 gatctcgcgc agcaacctct cggcgtcctg aatgatggcc tcatagtccc tgctgcggtg
    11101 gctcatctcc atgacggaca tgccggagtc ccggtactcg agcagctcgg atgcggccgt
    11161 cctgagaacg gattcgggca gcaccgcggg gcccgcggaa aaattgaata cgcgcgacat
    11221 ggcgattctc cttgtacgcc ctgttgtgga ttccggcggg cgtgcatccg gccgtacggg
    11281 caggggtcga tgccgcatca catccgtttg gcgtgccgta tgatgggcgt cgagcgcggt
    11341 gttgtcaaat gttttcgaat gccggggcgg gattattcgc ccccggaaac ggcatgggat
    11401 gcgcgcctag gacggcctga tcccgtcggc gatctccgcg atggcgcgat agagctcgtc
    11461 catctccgcg tccgccaggc cgccgccgcc gtacttgctg ctgaagagcc gttccttgac
    11521 cgatcggatg ttcgccgcgc gcaccgcatc gagtgcgccc tcccgggcga gccgctccag
    11581 ctcattgatc gcgatctcgg ccgtcttcag gaagaccggc gcgtcccgcc ggcgcgaggc
    11641 cccggagagg tcatggagcg tgcgatcgat cccgccgcgc ccggccgccg gcgccacgcg
    11701 tttcggctgt tccgtccgat tgtcgcgcgc ggcgatctcc cggaaccggc gccgctccca
    11761 cagcaccacg gccgccacga tcgccgcaac ccccaggagg gccagcgcga tcgccaggta
    11821 gttgaggtcg atcgcgccgt ccgccttctt cgccgtctcg atcgccgtcc cggcctgccg
    11881 cgaggcgtcg cccgtcgcct cgaaggcgat gcgcccggac gcgaccgatt cgtagcgcat
    11941 gctctcggga ttgaaaaagt tcagggtaaa gccgcccagg tcgaatgggc ccgagcgttc
    12001 ggggatcagc gagaacgtga attccttttc gccggcgagc gcgccctgct cgatgcgcag
    12061 ggtctccttc ccgtcgctgc cgatcacccg gatcgtgtcg cccgcctcga ttgccgcggg
    12121 tcgcacgagc gagatcaggt tcccgcgacc gcgcaccttc accgtgagct tcagctcctc
    12181 gaaaaccggc gtccgtccgg gcgggctcga cgcctcgata gtgaagctgc ccacgttccc
    12241 ttggaagtcg tccggcttgc ccgcctccgg cagggcgcgc acgctaacgc gctcctcgcg
    12301 gaacaggacc cgccgctgcc gcggaaagga aaacccgaag aactcgtccc gcgagcccgc
    12361 cacgacgatc gccgagccgc ccccgatcgt gaactccccc gactgcgtgg gtatgagcac
    12421 ataggtcgcg aagtgcgttt tggcgagctc cgcgccgtcg cggttctcgg cgcccgcctc
    12481 gagccgctcc tcgacggcct tttgcacgaa tcccttcgtc tcgggcatct tctcgggcat
    12541 ggggttctgc tcgaactcga cgccggagtg taaaataaaa tagcgcagca cgaccggttc
    12601 gcccacgaac accgcgcgct tcgagcactc gatcgaaccg tacaggcgca cggcgcgcga
    12661 gcccgggcgc aaattgtccc gcgggcgggc ctgacccgcg ctcgcgtaca acgtcaccgc
    12721 gcgcgacgag aggcgccgcg acccggcgcg gatcgcgacc gacgggatca cgaaggtccc
    12781 gggccgctgg gggctcaccg cgaaggtgag caccacgccc gcggatttct tcatttgcat
    12841 gttgatgatc tgcacgttgc tgaagtgctg ggcgccgttg aacgatatgt cgagcccggc
    12901 gacccgcggc gcggagaccg gctcggcgtc gtcgacgtcg ttcacggtga tccgcagcgc
    12961 ggcggtatcg cccacggcga tgcgcgaggg gaccagctcg gtctcggaca ccgaggccgc
    13021 atgcgcgggc gcgccggaga ggccgagcgc gcacagggcc gcgagcgcgg cgcggatcat
    13081 cctaccacgg cttttccagc atgcggatgc catcgccctt ccccttcgac cggcgcaccg
    13141 gcttgttctt catggattcc aaaatattct tcacctgctc gcggctcatc gcgccctgcg
    13201 ggcgctgctg ccggtcgtcg ccatccttgt ctttcgcgtc cttcttgtcc tgcggcttct
    13261 tccggtcgtt cgtatccttc cggtcgttct tcccgtcgga gccgtccttg ccgttcttct
    13321 gatcatcctt gttctgctgc tggttctttt tcaacaagta ttcgagattt ttcttgggcg
    13381 cctcgtaggt cgggtcgatc ctgagcgcgc tcacataggc gtcgatcgcc tcccggtatt
    13441 tcttttcctt gagataggtg ttgcccagat tgaagaacgc gcgcttctgc acgtcgcggt
    13501 cctcggcctg gagggcctgg ctgaaggcgg ccgcggcgtc ctcgtgccgc tcgagcatgt
    13561 agtcggcggc gccgcggttg aagcccagcg tcgtgcgcgc gcgctcgttg ggcgcgtagc
    13621 gggccgcgtc ccgatagcgc tccttcgcat cttcatattt cttgtcgcga aatttccggt
    13681 tgccctcgga cacgcgatcg cggaagggat cgagccaggc gacagtcagc agcgccagcg
    13741 caatgacgag cacccgcggc atcatacctc cctcctgcgt cccggcagca tgatttctat
    13801 cgcgagcaaa acggccagca cgagcgcgaa gatctgatag cgttcgttcc gctccttgac
    13861 gacgcgcgcg ccgtattcgt tcgcggacat ccgggacatc gcccgcgtga tccggtacac
    13921 gcccgccagg ctctcggtga tatccacgta ttcgccgccg gtccggctcg cgatcctctc
    13981 gagcgtctcc gtgttcttct tcgtgcggat gagattgccg cccgcatcgc gggcgtacga
    14041 atcgcccgag gggtcggcct cggccagcgg gatgtaatcg ccggaggacg cgccgatccc
    14101 cacggtgaag accgtcacct cgagcttcgc gaagcgctcc atcgccgcct cgagcgcgcc
    14161 ctgatggtcc tccccgtcgg tgatgagcgc gaacacgcgc gaggtgcggc gccgcttcgc
    14221 aaacacgcgc tcggcctcgc tcaccgcggc gcccaggtcg gtgccctgca gccgcatggc
    14281 atccggcgag gccgcgtcga ggaacatcga gaaggcgccg aggtcgttgg tgagcgggca
    14341 ctgcaggaag gcgtcgcccg cgaagaggac caagcccacg cggtcgccct tcacggaatc
    14401 ggccatgagg cggatggcgt ccttcgcgcg cgccaatcga ctgggcgaca cgtcgcgcgc
    14461 catcatgctc atgctcacgt cgagcgcgat gagaaggtcc acgccctcct tgcgctcctc
    14521 gcgggcgctc tcgccccatt gcgggcgcag gagcacgatt cccgccagca ggatcgcgag
    14581 cgcgaccagc gccgtcttca cgcgcgcgat tattggacgc tcgcggagga tgaggcggcg
    14641 catgcgggga tcggcggcca cgcgcgccat caccctgttt ttccaaaaaa gatacagtcc
    14701 gtagaaggcg agcacgatcc cgcaggcgag cgcgatgtag acgacgtatg ccaggtttcc
    14761 gaaatgcatc acggcacctt ccgatagaac accgagcgca gcaggatctc gaggaagaag
    14821 agcgcgacgg cgatgacgag gagcgcctgg aacttgtcga aaaactcgta gtagaccttc
    14881 gcctcggcct cggtccgctc cagcgcgtcg atgtccctga tgttctccca gagcacgccg
    14941 ctcgaggccg cccgatagaa cttgccgccg gtgatgcgcg cgatctcctt gaggccggtc
    15001 tcgtcgaaat ggttcttcat gaagcgctgg ccgcccagga agcgcgccgt cgccgggtac
    15061 ggcaccaggc cgtccttgcc gatcccgacg gcgtacacgc ggatgcccat ctcggcgcag
    15121 gccttcgcgg cggtctcggg atcgatgatg ccgcggttgt tcatgccgtc ggtgagcaga
    15181 aggatgatct tgctcttgcc cttgaggtcc atcatgcgcg aggcggccag cgagagcgcg
    15241 tcgccgatgg cggttccgtc ctcgcgcacc gtgtcgaagt cgacctcgtc gatgatgtcc
    15301 gcaagcaccg cgtggtcgac ggtgagcggg cattgcaggt aggcgtcgcc ggagaagacc
    15361 acgaggccga tgcggtcgct cgcgcgccgc gcgatgaaat cgcgcaccac ctgcctggcc
    15421 acctccaggc ggttgcgcgg ctggaaatcc tcgcccatca tggagtcgga gaggtccagc
    15481 gcgatcatga tgtcgatgcc caggtgcttt acgctcgaat agtgcacccc gcgccccggg
    15541 cgcgcgagcg cgacgatgag gaggaagatc gttaggaagc gcagcgccgg gagataccgg
    15601 taggtgcgca cgcggatcga ctgccgctcg cgcataacgc gacgggacga tatggcgacc
    15661 gccgcctccc ggagatggta ccgccggaaa taataccaga acaccatcgc cgcgtaggga
    15721 agaagcagca gcaggaggag cgggtttttg aaatcgggaa tgtacatgcc gtgcatcatc
    15781 gccggtgccg gagggtcctc accgggcggc ctccgtgtcg ccggtttcgt gcgccgcgcc
    15841 gtacgggcga tactccatcg cgagcttttt cgcgagggcg accgtcgatt ccatgttccc
    15901 ctcgaggatc tccttcgagg gcacgaactc cgcgaacttc atcaggtccc agaggttcag
    15961 gacccggtcg atgtcgtccc gatatttccc gatcgaggcg cgccccggga gggtctcgag
    16021 atagcgtacg atctcgcttg cggtcatctc catggcctcg gtgcggaact ggccggagag
    16081 aaacgtgcga aaggcgctcg agagctcgac gcagaatgcc tcgacgtcgc cggactcaac
    16141 atagccacgg gtgcgcaggg tgtggatgtc gtccatgaag cgctcgatgg gcggcaccgt
    16201 cgcggcgggc gctctcctgc ggcgtcgccg tgcgatcgcg cgcgcgatga acacggcggc
    16261 cgcggcggcg gcgacgagcg cgatgacgag cgcgatcagg cggtagtagt ttccgccgag
    16321 ctccagtggc ggctcgatct cctggaactc gcccttctcg ttcacgggct tcaccgtgac
    16381 ctcgggcacc ttgtagccga ttttgattcc gtcctcgccg cgcagctcga tctcggggag
    16441 ccggaaggtc cccgtccggt agaaggtgac cgtcacgcgg atcgtctgat agcgcatcgc
    16501 cgcgtcggag ttgtcctccc gcgccgcgtc gcccaccgcg cacaccggga tcagcgcggc
    16561 ggggtcctca tccttcgctg cggccgcgtc gcgcttcccg gcggcctttt tcggctccac
    16621 atatccgggc aggtccttcg ggaaaaactc ctttttgtcg ggaaggacgg ccgtcacgcc
    16681 cgcgatgttc tttcccgcga tggtcacggt atagacgacc ggcgcgccga tggtcgtttc
    16741 cgtcggattc gccgcggcgc gtacctcggg atagtcggcc gcgtatccgc ccgtgagcgc
    16801 ggcgagcgcg agtgcactgc tgatgacgat ggcggcgcgc agggtcgtca tggcgtccgc
    16861 tactgccgcg tgaggatggt gcggttgcgc ttctcgaaga agtggagcac gggaagctcg
    16921 atgggcgcat ccgtcgagag attgatcgaa tcgaactcct tgaggaacgg gagcgtcccc
    16981 ttctcgggaa cggtgtccga gaggaacgcc tcgccggtct ccagatccac gaactcggca
    17041 agcccgaaga ggcgcagctc cttttccatc ggatcggaga tcaccacggg gatcacgtcg
    17101 tgccgccgcc gcaggagctt catcttgagc aggtagtcgt cgtccaggaa gtccgacacc
    17161 atgatgatga cgctgcgctt cttgagcacg cgctggagga agtcgatggc cgcgccgatg
    17221 ttcgtgcggc gtcccgcggg gcggtacttc acgagctcgt cgagcaccga gaggacgaac
    17281 ttccggccct tccggggcac caggtatttc tcaacccggt ccgtgaacag cagcacggag
    17341 atccggtcgt tgttcatctg cgccaggtac aggaagagcg tggcgatgtc catcaccacg
    17401 tcgcgcttgg agcgcgcgct cccgaactcc accgaggccg agaggtcgat catgagcacc
    17461 accgacagct cgcgctcctc gatgtactcc ttcacgaaca ggtggttcat ccgcgcgctc
    17521 acgttccaat ccacgttgcg cacgtcgtcg ccgtactggt actcgcgcac ggaattgaag
    17581 gacagcccca tgcccttgaa ggccgagtgg tactcgccca cgaacacggt gtccagcttg
    17641 tggcgggtcc tgagcttgat gcgccgcagg accgatcggt cgtcgccgcg caccttacgg
    17701 cacctccacc gaatcgaaga ccatgcgcac gatgtcgtcg ggcgatttcg cgtcggcctc
    17761 ggcttcgtag gataggatga cgcggtggcg cagtacgtcc gcgccgatct ccttgacgtc
    17821 gtcgggcgtc gcgaagccgc ggccgcgcat gaaggcgatg cagcgcgcgg ccttcatgat
    17881 ggcgatggag gcgcgggggc tcgcgccgta ctcgatgtag cggcgcaggg cctcgtcccg
    17941 cggggcgcgc gtctcgttca cgatgcccac gacataggcg acgagcttct cgtcgaagta
    18001 gaccgcgtcc accagcgcgc gccgatcggc caccgccttg ggcttcacca ccggcgttat
    18061 gcggtccacg ttgatctcgc cgaggcgcga gagcatctcc cgctcctcgg cggcggtcgg
    18121 gtagtccacc agcgtcttca tgaagaagcg gtcgagctgc gcctcgggca gagggtaggt
    18181 cccctcctgc tcgatggggt tctcggtggc cagcaccatg aagggcgagg gcagcgcgtg
    18241 ggtctcctcg ccgatggtga cctgccgctc ctccatcgcc tgcaagagcg ccgactgcac
    18301 cttcgcgggc gcgcggttga tctcgtcggc cagcaggatg ttggtgaaga tgggcccctt
    18361 gcgcgtgaag aagttgaggt ccttggggct gtagatgcgc gtgccgatga ggtcggccgg
    18421 caggaggtcg ggcgtgaact ggatgcggct gaagcccgtg tcgatcgccc gggcgaaggc
    18481 gcgcaccgtg agcgttttcg ccaggcccgg cagcccctcg atgaggatgt gcccgccggc
    18541 catgaacgag atgatgatgc ggtcgagcag ccgctcctgg ccgatgatga ccttcgccat
    18601 ctcctgcttg atctgcgcga tgaagccgtt ctcggccttg atcttctcgg tgagtttttc
    18661 tatctgggcg tactccatac gggcacctct ttcgctcgcg ttccgtcgtc ccgggaatgg
    18721 gggttgggtg ttgttcgatg cgctttcgcg gaaaaccgtt atcgggattt gtgtgcaaaa
    18781 tggcaagcat ttgttacaaa aaaccccgcc cggtctcccc cgtcgcacgg gggagaccgg
    18841 gcatggcatc gagaggcaac caatcgtctc agtcttcatt gtgatgcggc gatggccaca
    18901 tgcgcggaaa tttttgcaac cgcccgggtt caaggctgtc cgtttcttat aaatcgcgag
    18961 gtctcatcaa cacacagaac gcaacgacgc acgctgcaaa cggtaacgat tgtcaggaga
    19021 aaacgatggc aggggaaaag ctgttactca cgggcgtgtt cggcccgtat ggcgtctcgg
    19081 atgaatacgc cgctgggctc ggcatgcaga tggagctgtt gaacaaccag atcacgcgcg
    19141 agcagggggt ccactcgccg cgccagtcgt attggacctt cccgctgtat ctcttggcgg
    19201 agaacctcgc ggtctccagc accgtgctcg attttccgag ctggagcgag tttacgcgcg
    19261 aactgaagaa gggctacacg cacgtgggca tcaacttcat cgtccccaac gtgatgaagg
    19321 cccggcgcat ggccgagcat atccgccgcc atcatcccgc gatgaagatc atcctcggcg
    19381 ggtacggaac caccatcccc gatctcgaga ccatcgtgcc gcacgatgcg aagtgccgcg
    19441 gcgagggcgt acgctggctg cgcgagtatt tcggcgagga ccccgccgcg ccgctgcggc
    19501 atcccgtgat catcgggccc gcctacgagc gcatctatgg cttctccgga aagcccagcg
    19561 gcgggatcct catgcccggc gtcggctgcg agaacggctg caaattctgc gtcacctcgc
    19621 acaacttcaa caggcagtat ctcccgctcc tgcgcacggg aggcgatatc ttcactgcct
    19681 gccggcgcat ggagacggcg gtgggcgcgc gcggcttttc cgtcatggac gaaaacttcc
    19741 ttaagaaccc gcagcgggcg cgcgagcttt tagaggtcat gaccgagcac aacaaaccgt
    19801 acgtgttcga catcttctcg tccgcggagg tgatcgccac cgtgggggtc gatttcctgg
    19861 tgcgcctggg cgtgcacctg gtgtggatcg gcgtggagtc gaagcggagc tccttcgaga
    19921 agaccaaggg gatcgacctt tccgcgctca tcgcggagct tcgcgccaag gggatcgcgg
    19981 tgaacgcctc cgcgatcctc ttccaggacc accacgacga acagacgctg cgcgaggaca
    20041 tcgactacgt gatcggcctg gaatcggacc tggtgcaatt catgaactac acgccgcttc
    20101 cggcgaccgc gctcaccgcg gagctttcag cggaggggag actcaacgac acgccgtatc
    20161 gcttcctgca cggcgcgggc ggactcgcgt ttgtccaccc gcacatcaac gaccgaagga
    20221 agcatgtccg ctaccttcgg gaggctttca ggcgcaagtt cctggcccac ggccccggcg
    20281 tgctcagcat ggcgcaaacc gccatcgccg gataccgccg ggcggtcgcg gaccacgcgc
    20341 ggcgaatgcg cgacggtctc gcctggaacc ccgcgacgct tcgctacgag cgaaccgata
    20401 cgcccgtacg cgacgagttc atggagtcgc gcatccgcat gatgcgaaag atcgccacga
    20461 acatccggct cgtcctcacc gcggcccgcg tctacgcgcc caacggggcg gcccgcaaaa
    20521 aggcgaaagc ggtcgcgcgc gacttcaccg aaacgctcgg cccggcgccg cttcccgcgc
    20581 gtctcgcctc gctcgccttg gtggccaccg ctgccgtcga atggctgcgc acccgggccg
    20641 cgcgtctggc cggacgcgag agcgtcatct accaaccgcc gtccaaacgg gtcgagtatc
    20701 cgcgcgcgat ttcgctcaca aaggaacacg cgacaatgcg cgccctgctc gacatggttc
    20761 ccgaattcgt gctgggcgcg atggcccgac ggaacatctc cgtgcgcgca ctcgcccggc
    20821 gcgccggcat ttcgcccgcg ctcgtccgcg gcatcgtcgc cggtcgccga cacgacgtga
    20881 ccgcccatac gatcgaggcg gtcctcgccg cgttgggata ccggatcgtc ctggaatagg
    20941 gctgggcgtc gtttgctatc ggacgctggt acattctgaa ttgtatattg tctttttgtt
    21001 ttgaggctga tgacggcttc gccccggtct gtttttgcac atttgcatgg aaccagtacg
    21061 aacacaaagc gaactcaaaa tgaatattct atcggattgt gttgtatttt tttattgcaa
    21121 atgctggctt cgatacgcct gcggggaaag acatctaccg actgcataac agattcatca
    21181 tttaaccatc accgtacaag acttacaatc ctatacccgg aacttaaatt tcacattccc
    21241 cctgaagagc ggccgaacca acgcaatccc gaggatattg acgagaacgc ctgtatccgt
    21301 aatgttttgt ttgtcggcaa taggacctcc atggatggaa ggacgtcttc ggccgtatgt
    21361 cttgggccaa caaccagcta tggcgcatat gcacccagct cccgaatcac gacaccccct
    21421 ggaatcagag gttacatcag catctgtgcg gtgaaaagaa ggacaagaat tgacatccag
    21481 cgatgcgtcc aaacggaact atcgagccgc gaggattgca ttcgatgccc cgttccgtgc
    21541 ctcacttttt cttgagcgac tccagcagct ttttaatccg ctcgcggttg tcgtcggaac
    21601 ccacataggt cctaaacagc cactcgtgca ttttcatggc gccgtcgacg ctgttgttca
    21661 tggcattgtg tgcgaaccgt ttaatctcca tcatacacat gcggtcatag gatgccatcg
    21721 tccgggcgat ttccataacc gacgagagca cggtccccgg cgtaaaaagc ttcgtgacat
    21781 accccaagcg cagcgcctcc tgggcgtcga agatccgccc ggtcagggcg atctccttgg
    21841 ccggccccat cccaatgacc cgccagagcg gatcgaacat cggcacgagc gagaggagca
    21901 cctcgcgttg gctgaacttc gaattctctg cggcgtaccg gatatcgcac atcagcgtga
    21961 ggtcgaaccc gcccgcgatg gcggcgccct cgatcgcggc gatgacgggc tgcgcgcaga
    22021 acatgatgga acgataggcc cggtggaaga gtgcggtgaa cgcctcgttg gactccctcc
    22081 gaagcgtctg aacggattcc agatcgaagc caccggaaaa gattttactg cttccggtca
    22141 gcacgattac gtggacgtcc tcccgctggt tgaggtccgt gaagatatcg ccgatctccg
    22201 tgaggacctc cggcgaaagc gcgttcccct tgtccggacg gttgatggtg accagcgcga
    22261 tcttgtcttc gattgcggtt atgagatatt tctgattcat ggtatcctcg tcctcgccat
    22321 ccaacattat acccaagtgt agaaaacgac cgacatcgtc gtcccacaag agcctagcac
    22381 atcccaacag gcgcgacgaa tactctgtaa ttgttgttgc gctggcgtca acccgaactc
    22441 accatacttc gccagatccc ctgcacgttt cgccaacccc ctcccacgaa aaagccccgc
    22501 aacatcgcgg ggctttttca ttctcgttcc tgcgcgtcct actcggccgc cttttcctcg
    22561 gccggcgctt ccgtcttttc gggacctttg gcctttacgg ccttcgggtt tttcgggatc
    22621 ttcagtttca tgcccggccg gatgaggttc gggttcttga ccttcccccg gttggcgtcg
    22681 tagatcaccc tccacaggcg cgggttcttg tacgcctcgg cggcgatgcg ccacaggcag
    22741 tcgcggcggg acggacggta cttcaccttg tagatgtagt agtccttgtc cgcatcggcg
    22801 attttatcct tgtccttgtc ggcgtcgggg cggtccttgc ccaccgcggc gtcggggtcc
    22861 tgtgcgggct tcgaggcgac cacgatgccc gcgaggcgtt tcgcctcgtc ggcggcgtcg
    22921 atcgcctcct tgtacttgcc ttcggcgaac aggcccttcg cgttcttgat cgattcgttc
    22981 gccccggcga gctcgtcctt cgcgccgctg gcgcccgcgg acttgttggc gttctccacc
    23041 acgagctcgg cggccgagat gcgcttcttc gcggtctcct tgagcgaggc gaggtaggcc
    23101 tcgtcggcgt tgatcttggc gacctccacc gcggacagcc ccttcttgag cttcatgtcg
    23161 tccaacgcgc cctgggcgat tcccaggttc tcctcggcga gcttcacctt ctcgggggcg
    23221 tacttgtcgg cgtcgtagcg ctttgcgtcg gccagcgtcg agcgcacctc gtcgatggaa
    23281 tccttgagca cgttcttctt gcccagggcg atcgtgcggg cgttcttcgc ctccttgtcg
    23341 gcctccacgg cgcccaggta ggcctcgtag tatttcttgt tctcgaactt gtcctgcgcg
    23401 tccttcacca gcttctcggc aacgccgtac tccggcttgg ccaaaagcgg cgcggcggcc
    23461 tcatcggcct cctgcaggct cttctcggcg atttcgatgg tgtcctttgc caaaagcggc
    23521 acggccttgt tgtaggcctc gatggccttc ttctgcgact cgacggcggc cttggttgcc
    23581 ttctccaggt cttccttctt cacctgctga tggcactcga agagcttctc cttcgacgcc
    23641 tggagctcgg cgggtgcgta cttgtcggcc ttcaccgata tcgcgcgcga catgtcggcc
    23701 ttggcaagcg acatctcctt gatgggaacg gtctcgcggc agccagcgac gaccgcgcag
    23761 agtaccgcaa ggcacagtga atagactttt atgcttcgtt tcatacgccg ccccttttgt
    23821 agcaattatt ttgatgatgg tgccggccgc gcgcgaggcg cgcgaatcag cccggcgatg
    23881 ccggctattt gttgaatgaa tccaccgcga cgtcctcggc gtcgaagatc tcgaagaacg
    23941 acgtgagctt ggtcagctcg aacaccttgc gcaccgacgc gtatacgttg atgatcttca
    24001 ggccgccctg gtacttcttc aggttcgaga ggctcgagat gagcgccccg atgcccgacg
    24061 agtctatata cgaaaccttc tcgaggttga tgatcacgtt atatttcttc tcttcgatga
    24121 gcttgctgat gatatccttt atctccggcg cattatagag gtcgatttca cccgtgatgt
    24181 cgagtataac gatgtcccct ttgttcctct tcgtgatatc catgctcgac ggctccttct
    24241 ctgttgtgga aatatgcctt ccgtgtatca gcattccacc ctagtaggct ctggcggaga
    24301 tgtcaagata aaatattctg aaaaaaccaa tgaattcgat aaaatgttca ggttccgaac
    24361 tcagtcgccg ccggtatcgc ccccgccggg agcgtaggcg cgctcgtact cgcgcatcag
    24421 ggcgccaaac gaaccgccgg cgatcgatgc ggcgatccgg tccatcaggc gcttcatgaa
    24481 cgaaaggttg tgataggtat tgtagatgag cgcggcgatc tcgcccgcgc ggaagatgtg
    24541 acgcagatac gcgcggctga aggtccggca cacgtaacag gggcacccgg cgtcgatggg
    24601 cccgtcgtca tccgcgaagc gcgccgtttt aatgttgatc cggcccgcgt ccgtgtacag
    24661 ggtcccgttt cgcgcgatcc gcgtgggcat cacgcagtcg aacatgtcca cgccccggct
    24721 cacggcgtac aggatctcca gcgggctccc cacgcccatc atgtagcgcg gcttgtgcgc
    24781 gggcagcaga ccaagcgtca gttcggtcat ggcgcgatag agctccttgg gctcgcccac
    24841 ggacagcccg ccgatcgcgt aaccggacac gtccagctcc agcagccgct cgatgcactc
    24901 gcgccgcagg tgctcgtaca ccccgccctg ggtgatggcg aagagcgcct ggcgcccggt
    24961 gtcgaaacgc tcgcgccagt agccgtgcga gtcgcgcgcc caggcgacgg tcttctccag
    25021 cgcgccgcgc gcctgcgcct ccgtcgccgg gtagtggatg cattcgtcca gcaccatcat
    25081 gatgtccgat ccgatggcgc gctgcgcgtc cagcaccagg cgcggcgtga acaggtggcg
    25141 cgagccgtcg atgtgcgagc gaaactccac gccctcctcg cgcaccgtgc acaggttcga
    25201 gagggagaag acctggaacc cccccgagtc ggtgaggatg gcccggtcga agcgcatgaa
    25261 ccggtgcagg ccgcccgcgc gggcgagcgc atcggccccc ggccgaaggt acagatgata
    25321 ggtgttggcc aggatcatgt cgaagcccag ctcgtcgatg tcgcggttgg tcagggcgcg
    25381 cacggcgccg cgcgtcgcga ccggcatgaa taccggcgtc gcgatctccc cgtgcgccgt
    25441 cgaaagcgtc ccggtccgtg cgcccgttgc cgtgtcggtt gcgtgtactt tgaattccat
    25501 gatattattt tcgatgaaaa tttttcattg acacaaagga gcgcctcggt agtgtggcga
    25561 ctgctttacg gggtgttagc tcagtcggtt agagtacctg cctgtcacgc agggggtcgc
    25621 gagttcgagt ctcgtacacc ccgatccgta gtgcatcaaa ggaggcgtta gcgcgcctcc
    25681 tttgcatttt caccctcgac ccgcccgcga agcccctccc gccgcacctg aaacacgacc
    25741 gcgcagctcc gatcgtcgcg aaacgagcga tacacgatgg ttccgcgcgc aatcgcgtcc
    25801 cgaatgtcgc gatcgaaggt gagctgcagc cacgcccgct gccctgcgcc ggatgcgccg
    25861 agggccttcg ctcgctgatc ggcaaaatcg cgcaaaaccc gctcgcgggc gagctcggcg
    25921 gcgcgctcca gcgtggcgcg ccggctcacc ggcgccgcgc tcccggcgga cgctccgtcg
    25981 cgcgcaaccg cgggcgcctc aaccgccacc cggtatatat cgtcgcccgc ccaccccgtc
    26041 tgcggcgcac gcggcccgct cgcgcagcgg gcgacgacgg ccgcaatcat aacaacggaa
    26101 aaaagggcgc cccgtgaacg tcgcatgtat tgctagccgc cgatcttcga catcgggcgc
    26161 accggcgccc cctcgagcga tcggcacacc tggccggtgt tgctgtcgca caccagcgta
    26221 tgcgcgcacg gcttctcggc caccgcgcgc cgaatgatcg tccggagcgc gtcgtcgccc
    26281 atgtccatgc gatacgggcc cttgaggtcg tactcggcgg ttccgtgcaa gcaggtcttg
    26341 agcatgccgt cgcaggtgag gcgcatccgg ttgcaccgcg cgcagaaggt gtgggtgatc
    26401 ggcgggatca cgccgatgcg cattgcgcgg ccgccccgat actcgaggtc gtacatcatg
    26461 gcgacgctcg tgtcgatgcg gtcgttccgc gcgagccgcc ccagcgccgc gaggcgctcg
    26521 acgaggctgt cggcgggcac gaagccggac tccgcccgat cgtccgtaaa cggcatctgc
    26581 tcgatgaaac gaagcatcac gtccttgtcg tgaaagaact cgaggaagtc gttcacctcg
    26641 tgcagcgaat cctcgaacag caccgcgttt atcttcaggt cgaacgcccc cagcgcgatc
    26701 gcccgctcga tgttcgcgag cacgcggtcg aagtagtcgc gcccggtgat ctcccgatag
    26761 cgcgcgctcg acatcgtgtc caggctgatg ttgagcttgc gcacccccag gcgcatgagc
    26821 tccgcgagcg ccccgtcgag gagcaccccg ttggtcgtga ggcagagctc gataccggga
    26881 gcggccgcgc gcgcgccggc gatgatgtcg atgatgccct tgcgcaccag tggctcgccg
    26941 ccggtgaagc ggatcttgcg cacgcccagc tctgcgaaga gctcaatgaa gtgaaggaac
    27001 tcctcgttgc gcaacacctc gtcgtgcgtc aaatgctcca cgccatcggg cgggatgcaa
    27061 taccggcagc gcaggttgca cttgtccgtc accgagatgc ggagataatc gagcacgcgg
    27121 ttgtagggat cacgcaaaat catgtcgctc ttggcgctgc ggcgcctgcg ctccgttccg
    27181 tgttcggcgg ggagtcccgc cgtcgatggc cattctagcg cacgaccccg cggcggtcac
    27241 cgattttttt cttcgctaca gcgcgggcgc gacgggcggc gatacgcgct caacggcctt
    27301 cgcggctttc agcagaacgc gctcctggaa gtggcttgcc atgatctgca ggccgatcgg
    27361 caggccgcgc gcatcgagtc cgaccggcac cgagacgccg gggatggccg cgaggttcgc
    27421 ggagatcgta aggatatcgg acaggtacag cgagagcgga tcgctgatct tctcgccgat
    27481 gccgaaggcg gtggtggtgg tcaccggcgt gacgatcacg tcgacctccg cgaacgccct
    27541 ccggaagtcc tcgatgatga gcgtgcggcc cttgagcgcc ttgagatagt acgcgtcgta
    27601 atagcctgag ctcagcgaga aggtccccag catgatgcgc cgcttcacct ccttcccgaa
    27661 gccggcgctg cgcgtccgct cgtagagctc ggcgaggttc gccgcgtccg gtgcgggcgt
    27721 cccgtagcgg atcccgtcat agcgcgcgag gttcgacgac gcctcggcgg tggcgatcag
    27781 ataatacacc gggacggcgt actcagtgag acgcagcgag atggggcgga ccgtggcgcc
    27841 ctccgcctcg agcgcggcga tctgcgcgcg gacgcgatcg gcgatctccg gcgcaacccc
    27901 ggcaaaatac tcctccggca tgccgatccg caaaccgcgc acatcgcccg tgagcgagtc
    27961 tggcccgaaa tcgacgggcc ggtcgatcga agtggagtcg cgccgatcgt ggcccgagac
    28021 cgccgccaga agcgcggccg cgtcgtccac ggagcgcgcg aaggcgccga tctggtcgag
    28081 cgacgatgcg aatgcgacga gcccgtagcg cgacacgcgg ccgtaggtcg gcttgacgcc
    28141 cacgcatccg cagaaggccg cgggctgccg gattgatccg ccggtatcgg accccagcgc
    28201 cgcgggcgcc atgagcgccg cgaccgcgac ggccgaaccg ccggagctcc cgccgggcac
    28261 gcgcgtatcg tcgtgcggat tgcgcgccgg tccgaaaaac gacgtctccg tcgtggagcc
    28321 catcgcaaac tcgtccatgt tcgtcttccc gagcgtggcg aacccgtgcg cgcgcatccc
    28381 ggcataggcg tgcgcgtcat agggcggcac gaaatcgcgc aagatcccgg aggcgcaggt
    28441 ggtgcgcaat cccgcggtgc agatattgtc tttcacggcg acggggatcc cgtcgaagtc
    28501 ggagcgcgct tctccgcgcg cgcgtcgggc gtcggcctcg tcggcctcgc gcaggcagcg
    28561 ctcttggtcc acggtgatga acgcgttgag tcgcccgttg agcgccccga cgcgggacag
    28621 caggtcctgc gcgatctcgc gcgccgatgc ctggcggccg gtgagcaggt cggccagttc
    28681 gcgcagtccg ttccgatgca gcatgcgtcc ctccgcgtgc gcgccccgcg gggcagtcaa
    28741 aatatgattg acgtaaacgc caatgaatat agcctgcagg agacggaaag ccccgatccg
    28801 gcgcggaatg tcactaccga ttttgcacac aattcaagga gcgcaaccta aaatgtcaaa
    28861 caggaaactg aaaataacct tttcaatcgt cgtgaccctt gttctcatct tcagctgctc
    28921 ccggaaaacc accgggctca agaaatccga cgtccagccg gtcatttcgc tgttcctgaa
    28981 caagcacgtg cagttccgtg agctcaacga cgagctctcg cgtcggacct tcaacaatat
    29041 cctccggcaa ctcgaccccg gcaaacagtt cttctataag agcgacatcg cggcgctgtc
    29101 cgtctacgag aataaaatcg acgacttcat cgaaaaggag aaatacgact tcctctccga
    29161 gatctattcc gtctaccgca agcgcttcgc cgccaacatc gtgctcatcc acgagctcct
    29221 caaggggcaa ttcgacttca ccattgacga atcggtcacc atcgacccgg acgcgatcga
    29281 ctatgtctcg accgacgcgg agatgcgcga gcgctggcgc aagagcatca agctgcaact
    29341 gttgaattat ctggacgtga tcaacgacat gggcaaggcc aaggagaaga tcgtgaagcg
    29401 ctaccagctc ttcgagcggc gcgtgaacga ggtcgacgag gcgaagatga tggacgcctt
    29461 cgcgaacgcc ttctccacgg cgctggaccc ccactccaac tatctctcgc ccgaggatca
    29521 cgaggatttc atgatccaga ccaagctcaa gctcgagggc atcggggtga tgctgcgctc
    29581 cgaggacggg ttcgtgaccg tcgagtccat cgtccccacc ggcgcggcgg ccaagctgcc
    29641 ggtcgacctg cagctcaagc tcggcgacaa gatcatcggc gtctctcagg gcgacggcga
    29701 gatgcaggac gtcatcgacc gcgacctgcg cgatgtcgtg cggctcatcc gcggcaagaa
    29761 gaacaccgag gtgcgcctca aggtcgtccg ccgtctcgac gagaagaaca agcagcagca
    29821 gctcatcatc cccatcaaac gcgaggagat caagctggag accagttcgg ccaagtcgga
    29881 ggtctacgtg tcaccgcgcc ccgcggcgtc ggcgaagatc ggctacatcc gcctgcccac
    29941 cttctatttc gatttcgacg cggcccaatc gttcgacaaa aacacgaaga gctcgttcca
    30001 cgattttcgc gcgcagctcg cctcgctcaa aaagaccggc gtggacggca tcgtgatcga
    30061 cctgcgcggc aatcccggcg gggcgctcaa tgaggcggtg tatctcacgg ggctgttcat
    30121 cgacaccggg ccggtggttc aggtgaaaga cggcaacaac aacatctcca tcatgcgcga
    30181 cgacgagcgg ggcatgacct acgacgggcc catcgtcgtc ctcatcgacg gatttagcgc
    30241 gagcgcgtcg gaaatcttcg ccggcgcaat ccgcgattat cgacgcgggc tcatcctcgg
    30301 ccccacgaaa accttcggca aggggtcggt gcaaaactat ctcaagctgg ccgacggcgc
    30361 caagggcgcc gtgaagatca ccaccgcgct gttctatcag ccggtgggga cctccaacga
    30421 cctcatcggc atcaatcccg acatcatcgt tccggacatc agcgccgtct gggacatcgg
    30481 cgagcacaag ctcaagtaca ccatcaagtg ggagaagatc ccgtccgcgc cctacaagcc
    30541 atacacgaaa tatctcaacg gcaccatcgt ctcgcagctc atcagcgagt ccaacaagcg
    30601 cgtgagctcc agcaaaaagt tcgccgacct catccagcgc atcaaaaaac tcaaggccaa
    30661 ggtcaatgcc aaatccatct cattgaagaa ggaagatttc agcagcgaag aaaaagacct
    30721 gaaggaaata aaaaagaaga tgcgcgacga gaacgacaag aagctgatcg acctcgaacg
    30781 cgacctcttc ctgcaggagg cgttcgatat aacgaccgac tacatacgca tgcttaaacg
    30841 gtagtcggct ccccgccgcc tgtggccgcc tccccgcatt cgattacggg gaggtcgcgc
    30901 atccgtttca tatgatacga cgcggcggcc gccgggtcct ccccgccggc cgcgacggga
    30961 tcgatcccca gacgcatgag cgtccgcgac atgcccgtcc gctcgcgatc acgcgccacg
    31021 caacaccccg acccaacaaa cacgaccgtc tcgatcccgt gggtgcgcaa cagccggtat
    31081 aataggtcca cccgctcgcc cagggggcgt atggacgggc acaggggccg gtacgagaag
    31141 gcgtccagga tctccgcata caggtgtggc gattctgcgt tgtacgatat atcgaactgc
    31201 cgccgcccat tgcacaagtc gtcctcatcg acgcggcacc ccgcttcctc gatctcgtcg
    31261 aacacgcgcc aatcgaaaaa gaacccgcca tgaacgagcg ccggcagcgc ggccgctccg
    31321 tcattccccg cgggaaaatc gttcaacgcg tcgcgaatgg gcgacaatcg ctcgatggcg
    31381 tgggccggcg ggaaacacgc ggcatattcc atgatcgcca gcaggtcctc ctgcgaaagc
    31441 gccgcaggcc ggtcccgccg ctccgcgcag atcccgcgca ccagccgtcg cacggcgttt
    31501 cgctcctcga cggccgcggc gagcgcgtcc ggatccagcg cggcggcgtc cgggcatccc
    31561 aaccgctgca ggcaatcacg cagcgattca tgaagcttca ccgcggcgcc ctcgccgtat
    31621 ccggacggac tctcgaatac gataacgcgc gcatcctgcg gcgaattgcg cgcacagcgg
    31681 caacaggacg ccgatacgac catagcgtcg cagcatcccg gcgcaacaca tgcggcgtcg
    31741 ccctccgtcc cgcaggcgcc ggaaacagcg tgcgcaccgg cgaggatccg tgcggggata
    31801 cgcactggaa tcgtcccggc ggccgcgagc acttccatcg gtagaaatcc gcacaccgcg
    31861 ccaacgacct gcttcccttc gcccgttcgc aatgccgtca tgcaaccgtc ccattgctca
    31921 aggagcgcgc gaagcggatc gaccatgaac cccagacttg attgctgcaa atcgctactc
    31981 cgcctcgatc atcgtaacgc attcacggat aataaatttc tagaattgcg atgaagattg
    32041 aagatagtat cgtatttgta aagcgcgaaa ccccttagaa acagcgaagg cggggaagct
    32101 ctccccgcct tcgctggctc gttactgcat atgcgtactt ctagtacatg ccgccgggag
    32161 gcatcccgcc gggcattgcc ggggcctttt catcttcctt cttatcggtg atgagcacct
    32221 cggtggtcga gagaataccc gcgaccgacg atgcgttctg aagcgccgtg cgaacgacct
    32281 tcgccggatc gatgatcccc gccttgacga ggtcttccca caccagcgtg ctggcattga
    32341 atccgatatt ggtcttctcg cccttggcgc gctcgacgat caccgaaccc tcgacgcccg
    32401 cattgctcgc gatcgtcttc atcggctcct cgaccgcgcg cacgatgatc tcggcgccga
    32461 tcttctcgtc gccctggagc ttgcccacga gctcgatcac ggatttctgc gcatacagga
    32521 gcgccatccc gccgccggga acgataccct cttcaaccgc cgaacgagtg gccgaaagcg
    32581 catcctcgac gcgcgccttc ttttccttca gctcgacctc tgaggccgca ccgacattga
    32641 tgacggcaac accgccggcg agcttcgcaa gccgctcctg gagcttctcc ttgtcgtagt
    32701 ccgaggtggt ctcctcgatc tgcttcttga tgacgctgat gcgcgcctgc acctctttcg
    32761 cttttccgcc gccctcgatg atcgtcgtgt tctccttgtc cacgatgatc ttcttggcct
    32821 tgccgagcat ttcaaccgtc gtgttctcga gcttgaggcc caggtcctcg gaaatcacct
    32881 ggccgccggt gaggatggcg atgtcttcga gcatggcctt gcggcgatcg ccgaagccgg
    32941 gggccttgac ggccacgcag gagatggtct tgcgcagggt gttcaccacg atggtcgcca
    33001 gcgcctcgcc ctcaacctct tcggaaatga tgacgagcgg cttgccggcc tgcgccacct
    33061 tctcgagcac gggcagcagg tccttcatgg tcgaaatctt tttctcgttg atgaggatgt
    33121 aggcgttttc gaacaccgcc tccatcgtat cggggttgtt caccatatag ggcgagatgt
    33181 aaccgcggtc gaactgcatc ccctcaacaa cctcgaggtg cgtgtcgatg gacttggctt
    33241 cctcaacggt gatcacgccg tccttgccga ccttttccat cgcctcggcg atgagctcgc
    33301 cgatctcgtt gtcgttgttc gcggagatgg ccgcgacctg ggcgatctcc ttcttgtcct
    33361 tgatctcgcg ggacaggttc ttcaccgcat cgacggccgc cttcaccgcc tggtcgatgc
    33421 cgcgcttgag gttcaccgga ttcgatccgg ctgtcacgtt cttgaggctc tcgcggtaga
    33481 tcgcggtcgc cagcagcgtc gccgtcgtgg tgccgtcgcc ggccacgtcg ttggtcttgg
    33541 tcgcgacttc cttcacgagc tgcgccccca tgttctcgaa gggctcctcg agctcgatct
    33601 ccttcgcgac agtcacgccg tccttggtga tggttggcga accgaatttc ttgtcgatca
    33661 cgacattgcg gccgcggggg ccgagggtaa ccttgacggc gtcgttcagc ttattgacgc
    33721 ccgtcatgat tgatcgtctc gcttcctcgt tatactgtaa aatctttgcc attgtctgtc
    33781 actcctctcc tgttagtcga tcactgcgag tatatcgttc tcgcgtacga tcaggaactc
    33841 cttgccctga tacttgacct cggtaccgga atatttgccg aagagcaccg tgtctcccgc
    33901 cttcacatcc agcggaatga gctttccgtc ctcgtagcgg ccgttgccga cggacacgac
    33961 tttaccctgc tggggtttct cctttgcggt atcgggaatg tagatcgaac cttgcttttg
    34021 aagatcgtca tcgatcactt caagaagaac cctgtctcct aatggtttga tagccattta
    34081 ctcctcctta ctatactttt aatgcgttga tagttatacc ctaattcaag cggacataga
    34141 gacataagtc tctatttagt tatgcatttg aattaagtaa atcgatgtgg ttttgttagc
    34201 actcgtcaaa acgagtgcta acaaaaataa atatatattt ttataacgat aattcaagcc
    34261 agatttaggc taatttaaag cgattattgc taattattga ataatataat aaaatacggc
    34321 gcaatatctc gaaaatgtat tttttaaata tcccgcgtaa tatccgactt cccgccgcgc
    34381 ttttcaacaa gatggatatt gcctagtacc attgatgcat caaccgcctt acacatatcg
    34441 taaacggtga gcgcggcaac gcttaccgcc atgagcgcct ccatctcggc tcccgttttc
    34501 ccgttgagtc ggatttccga ttcaatcacg acccctccgg ccggtcggtc aatgcgaatg
    34561 acgacatcga caaaggtgat catgagcgga tggcacatgg gaatgaggcc cgaggccccc
    34621 ttcgcggcga gaatgcccgc aacccgcgcc acttcaaacg ggtctccctt cggaagcagg
    34681 cggttttcga tcgcgtcgat cgtctcggcg ctcatcgaaa cgaagccgga cgcgcgcgcc
    34741 agtcggctcg tctcgcgttt cgccgagaca tcgaccatga ccgagcggcc gtccttttcg
    34801 taatgcgaaa aatcgctcat gacaacaccc ccatcaacac aaaggggccg acatcagtga
    34861 cggccccaca ttcgagatcg cccgttcgtg cggtcagtac ttatggtcct gaataaactg
    34921 aatgataaag ctcaccaacc ccgacaggat atagagcgcc agcacgatga agaccgacca
    34981 ttcacggaac aacagcacca gcagggcgaa aatggcgagc agcagaacga ttttcacgaa
    35041 gatatagcgc tggaaaaaag tggcctgcgg cttcgagtag cgaaccgtgg acaccatcag
    35101 gagcgcgaca agcgaaaaga gaaccgcgaa tatcacgcga tagatggtat cggcaagaat
    35161 gacgtcgcct atgaaaatgg gcacgacggc cacgatgatc ccggcgattg gcgacggcag
    35221 cccggtgaac gacttcgagt ccgaattgac gttaaagcgc gcgagccggt aggcggcgca
    35281 gataggaaac agggccgcga tgaagatccc gaaatcgaag gggatgttga tatacgggac
    35341 cgcgatgccg gacacgatga tgccagagag ataggctttg taggccagat agcccggcgc
    35401 cactccgaag gcgacgcagt cggccagcga atccagctcg gcgccgagtg ggcttgtgac
    35461 cttgagggcg cgcgcggcct gcccgtcgaa tccgtccagg agcgccgcgc cgagtatcag
    35521 cgccccggca atggtcgccg ccggcgctcc gccgtggctg gcaatgacga tagacatgaa
    35581 accgaggagg agattcccca gcgtgagaga gttcggcacc caggctagat tcatacacca
    35641 ccgcttgtgc agctcattgg agtgatcgga tcagggcatt gcgcgattca gcataatgaa
    35701 atcgaatcgg ggggttgtca acgaaaaaag aaatgctcct tacgccccga gcgctttctc
    35761 cagaaacgtc ctcagcgcgt catgatcggg gacgattgcg tcggcctcgg aaagatgcgt
    35821 ttcgtcgagc gtcgtccgta tggcgacgca ggcgaggccc gcgccccttg ccgagcggat
    35881 gcccatgggc gcgttctcca cggcgatcgc ctgcgccgcg tccacgccca accgcgcgag
    35941 gcaggacagg tacggctccg gatgcggctt tccattgcgg atgtcatcgc cggtgacgat
    36001 cgcctgaaac gcggtgcgca gcgtcggcgg cagcagatgc cgcaccgagc gtcgcatcga
    36061 tccggtcacc aggccgcgcg caatgtttcg cttcgccagc attgcgagga tgtccgcgac
    36121 gccgggaaac actcggacga cggcatgctc gaagattcgc agcttggcct cgcgtatgcg
    36181 gacaagctcc gcctcgtccg ggatcggcgc gtttttttcc gacaggatgt cgatgattga
    36241 cgtgatgccg ggcatcccct cccggcgaaa aatgtcctcg cgcgaaagct caagcccgca
    36301 gcattcgcgg atcgcgccca tccacgcatc ggcgtgatac ggcatcgaat cgacgatcac
    36361 gccgtccatg tcgaacagga ccgcgcgaca ccgctccgga aacgcacggg cggacacgct
    36421 atcgacccac cgacggcgcg cgcatcaaca ctccgttttt agagctgcgc gaacggcgtg
    36481 ccgctcgaat gcgagatcga tgagccggtc caagagcgcg gcgtacggaa gaccgctcgc
    36541 ctcccagagt ttgggataca tgctgatgct ggtaaaaccg ggaagggtgt tgatctcatt
    36601 cagatagtag gcgccggttt cgcgatcgag aaaaaaatcg acccgcgcga gtccggcgca
    36661 acacaggaca gtgaaggcgc ggcaggcgca ggagcggatc tcctccgcga gcgcggacgg
    36721 gacatcggcc ggtatcgaaa gacgcgcccc gtcgggatcg agatatttcg cctcgtagga
    36781 atagaactcg tggttcggaa cgatctcgcc cagcacggat gcggcaggct cgtcattgcc
    36841 caggacggcg cactcgatct cacgacaggg gacggaccgc tccacgagca ccttggtgtc
    36901 gaattgcatc gcaaaatcga cggcgtcaca aagctccgcc tcgatcttcg cttttctcac
    36961 gccaaccgac gatcccgcgc aggacggctt cacgaacagc gggaggccaa gccgtccgat
    37021 agcctcatcg accatctcgc gcttccgtcg ctcccaatcg cgacgggaca ccgtcacccc
    37081 gggagtcacc gggatgcccg cctgggcgag cagccgcttg gatacgtcct tgtccatccc
    37141 gatggcggat ccgatcacgt ccgcgccgac ataggggacc atcgccagct cgagaagccc
    37201 ctgcagcgtg ccgtcctcgc cgtaggtccc atgcatcacg gggaacacca catcgagccg
    37261 cacctcttgg ccggaaccga tctcgcgtaa cgtcaaacga ttgtcatcgg gatgattcgc
    37321 ggcaagccac gtgccggtcc gctccaaacg catcttgcgg ccaaactcag ggtccgggac
    37381 aacccccggc gcgtcctgca cataccagcg acccgcacgg tcgatgccga tcgccaccac
    37441 cgcgtaccgg ctcgcgtcca acgccgacac gaccgaggcg gccgagcaca gcgatacgtc
    37501 atgttcgccc gtcctcccgc cgaaaagcac cccgacggtg atcatcgccg cccgcgaccg
    37561 aggcctaaag cgtccaacgc atggaccgcc atcactggtt ctcttcgaga aacgccttgt
    37621 ggatgatctt taccgcgagc tccgcatatt tgctgtcaat gacgcaggag atgccgatct
    37681 ccgaggtcga gatcatttcg atattgacat tctcacggga gagcacctcg aacatcctgc
    37741 cggcgacgcc gtacgaagac agcatgccca ctccgacggc ggacacgatg gcgatatctt
    37801 cttttgattc caccgacgta ccgccgagat cgcccttgag gccgccgcac aattcttttg
    37861 cgcgggccaa atcgtccttt ttaaccgtaa acgatatcga cgccttgccg tcatgtcccg
    37921 tggactgcac aatcatgttt acatatatct tgttctcccc caggcatccg aagagctttg
    37981 acgcaacgcc cggtcggtcg acgatgtccc gaatggtcac cttggcctcg tcggattttg
    38041 cggtcacgcc gctgataacg aatttttcca tcatctcctc cctggatgtg acaattgtgc
    38101 cctcttccga gctgaaactc gatcgcacgt gcagccgcac atcgtatttc ttcgcaaact
    38161 caaccgagcg cgaatgcaaa actccggcgc cgagccgcgc gagctcgagc atctcctcat
    38221 agctgatctc gcggagcttc cgcgtccggg cgacgatccg cggatcggcc gtatagaccc
    38281 cgtccacgtc ggtgtagatc tcgcaatcgc ggacgcccag cgccgccgcg atggccaccg
    38341 cggaggtgtc tgatccgccg cgtcccagcg tggtgatatt gtcgtcacga tcgaccccct
    38401 ggaaccccgc cacgatgacc acgttgttgc ttctgagcgc gtcatggatc ttctccgacg
    38461 agatgctgtg gatcttggcg ttggtgaaat tcccatcggt caccatccgg atctgcgatc
    38521 ccgtgtacga taccgcagtc agccccgcct cgtggagcgc catcgccagg agcgcgatcg
    38581 atatctgctc gccggtcgag agcagcatgt ccatctcgcg cttgctcggt tgcgcggtga
    38641 tctcaccggc aagccgcacc agctcgtcgg tcgatttccc catcgccgag acgatgacca
    38701 ccaccgaata cccgttgtcg atgtacccct tgatccgctg cgcgaccgcc ttgatccgtt
    38761 ccgtggtccc gaccgaggtg ccgccgtatt tctggacgat tattttcata cgtacctgcc
    38821 aagcgcatcg ttcattgcgc ccaaaaataa aatcagggcg gattcttgtg aacccgcccc
    38881 ggttgtcaat acattattta ccgtatcgat tttacacggg gatacattta tggggagata
    38941 gcaacattac cacccccatc gattgtgaca acaacgaaat caccggcatt atcggtaaac
    39001 tcgggcagat ctcccgcacc gggggatgtt tggcgatggc atattttcca tgaagaattg
    39061 tacggcacat ttttcagcgt aaaggatcca tcactggtcc atgtcgccac agcaggaagc
    39121 aaatatgcat tacgcgaatc gttatactcg tcccacaacg tagaaaacga tccatcgcta
    39181 acgcatgcgg caaacagatt gtagttttcc ttgtacttct cgtacttcaa gtaataatcg
    39241 agatagcttt caatatcatt cggaatcggg acatgcggtc ctttcggatt atcgcactgt
    39301 gctggtggcg tgccggatgt tcgcaaggtc ggccgtacaa cggacggccg gttcaatgaa
    39361 gcgcacaagc tgtcaggccc aaccgcagcc acataagaat tggccacgac ggttatcccc
    39421 gtcgtgtgcg cattcgtgat gtcgaccgtt ttaccgcgca catacgaccg cgccacggcg
    39481 ataatattcc cgccaatcac cgcatcgtcg gcgaccacgt cccgcagcca gtccgaaggc
    39541 ccgaaatagc gatagatgta atgatagccg tcacgtacga aatcatactc atagcgcttg
    39601 acgaaatttt taaacacaag gcggatctcg aggaccgtct catcgttggc gtaattgaag
    39661 ttcaatccgc caataatcgg aattttcaaa ggaaagatgc gattgatctc tttcgcgttt
    39721 tgcttgaggc tgtcataata cgtgttcacc tggagtggat tgacatccag accttcgacg
    39781 ctgtccacat cgaagatcgt gtagagcttg ccatcgctcg tccatgtccc gatgcccggc
    39841 tcgccgggta tcagatgata ttgcatcgcc ccgtcgaaca ccattttccg caaaaacaac
    39901 cgcatcccgg tccacagacg cccggcctcc acgtcatcgt tcggatgacc gatgcccatt
    39961 ccgctaaaaa agggctccga gtcattgaga tcgagcgtgt acac
//
LOCUS       DAORHF010000005        37169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_573_length_37169_cov_5.597456, whole genome shotgun sequence.
ACCESSION   DAORHF010000005 DAORHF010000000
VERSION     DAORHF010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 37169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37169
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_573_length_37169_cov_5.597456"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="PKJ16_00965"
     CDS             complement(<1..139)
                     /locus_tag="PKJ16_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020311492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV45581.1"
                     /translation="MSEYIVAVILGVVEGITEFLPISSTGHLIIVNQFFDFTGPFAKL
                     FD"
     gene            complement(254..595)
                     /locus_tag="PKJ16_00970"
     CDS             complement(254..595)
                     /locus_tag="PKJ16_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV45582.1"
                     /translation="MGTHAGTIEEYARGGIALAGAIDVQQDAVVSKTLINKNAGTITL
                     FAFDAGQGLSEHTAPFDAFVYVVEGTVEITIAGSAHSVSAGEMIIMPAGKPHALRAVS
                     AFKMLLVMVKG"
     gene            complement(605..964)
                     /locus_tag="PKJ16_00975"
     CDS             complement(605..964)
                     /locus_tag="PKJ16_00975"
                     /inference="COORDINATES: protein motif:HMM:NF007714.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HNV45583.1"
                     /translation="MKRSSDVLERDIGTLANFIGIYCGRVHAEQKKKRVQAGGAVGTL
                     VNRHAVALCGECAALLLHAASKRLICPYDPKPNCKRCPTHCYAPGHRERIRAVMRFSG
                     MHLVRRGRIDLLLHFFR"
     gene            1116..1739
                     /locus_tag="PKJ16_00980"
     CDS             1116..1739
                     /locus_tag="PKJ16_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HNV45584.1"
                     /translation="MQKSHPSNRPDLTIQSLRAGEPITIVAIGDSLTNGWMVRKGYLD
                     FLGEMLRETYPKAQFKIINRGIPGDTADGGLDRLRGDVIDFDPDCVLVQFALNDMFMG
                     YPAAQYKNYLQAIIDSLINDTSAETVLVTSVCLGDTRENERANEFYEKMEELARENGV
                     SIARVHAHWARAIADGAAFRTLVQSDMVHPTVEGYRLMAEAIMQVFR"
     gene            1798..2826
                     /locus_tag="PKJ16_00985"
     CDS             1798..2826
                     /locus_tag="PKJ16_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV45585.1"
                     /translation="MKPLIGSALAAVFLMTMAPLLHADGELSSAELQGVPLESGRTIP
                     RLRIAYRTYGALNADKTNAVLFPTWLMGTSKELADLGYIGPGKAADSSRWFVIAVDNP
                     GNGVSTSPSNRARHGVTELPPMTVRDLVNVQRALVTGHLKLERLHAVMGISMGALQAL
                     EWSVSHPDMMTRVVAIAGTPRITTHELLLWNSQVALIEAARGMKDGNRTAMRLIAPTH
                     ALMSRTPEFFITQVPPENYPALLNATETALAAYRAEDWAWQVRAIVTHDIARPYGGSL
                     ARAAARVRARTLVIVSPGDRMVLPQGALEYARLVRAKTIENTGACGHFSFLCERDAIA
                     PRIRTFIE"
     gene            complement(2848..3006)
                     /locus_tag="PKJ16_00990"
     CDS             complement(2848..3006)
                     /locus_tag="PKJ16_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45586.1"
                     /translation="MVASDHDSASHYAESPAVEGALTPEEIRRMLAAYLERFMWINTV
                     PREKLPWE"
     gene            complement(3011..4285)
                     /locus_tag="PKJ16_00995"
     CDS             complement(3011..4285)
                     /locus_tag="PKJ16_00995"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HNV45587.1"
                     /translation="MKLVIKNGRVIDPASHTDDRRDVLVEDGFIAKIAANIADSVDET
                     IDAAGCVVLPGLIDMHVHFREPGREDIETIIGGSTVAAKGGFTSVCTMPNTVPPIDNQ
                     ALVRFIKLEAEQGPINIFPIATITKGGQGEEISEMGELVKVGAVGFSDDGKPVMSSIV
                     MRRALEYARMFDVPIITHSEDAQLANGGIMNEGANSVLLGLPGIPKAAEEVMIARDMI
                     LARLAKGRVHVAHVSSGGSVEIIRRAKSDAVRVTCETAPHYFALTDDAVEQHLSMAKM
                     NPPLRTEEDRQAIIAGLRDGSVDVIATDHAPHPINEKMQEIEYAPFGIIGLETAVPLI
                     VTVLVRENGFSLLQAFEKVTINPARILKLDRGALQEGAAADITVIDPEKKVLIDQAFI
                     ASKCKNTPFLGRELYGSVEWTICGGRVVYRKG"
     gene            complement(4287..5231)
                     /locus_tag="PKJ16_01000"
     CDS             complement(4287..5231)
                     /locus_tag="PKJ16_01000"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HNV45588.1"
                     /translation="MTGLNRKDLLDIQDLSLDEINLICNNARYFKDLFTRSVKTVPVL
                     RGKTVCCLFYEPSTRTKLSFELAAKRLSADLINIAVSTSSVVKGESLIDTVHTLEAMK
                     ADYIVMRHAVSLAPHFLSKNIGASVINAGDGFHAHPTQAMLDAYSILEKRGSLAGLHI
                     GIVGDIKHSRVARSDIEVFNKLGAKVTLCGPPTLVPDEFRQYGVDITYDLDGLIGKLD
                     VINMLRIQKERQKGSHFPSIREYHRQFALTNERLRRCKPDIIVMHPGPINRGIEIDDG
                     AANSANAIIEEQVTNGVAVRMSIFYLLAGGAPLEGIGE"
     gene            5470..6855
                     /locus_tag="PKJ16_01005"
     CDS             5470..6855
                     /locus_tag="PKJ16_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45589.1"
                     /translation="MLRLIMILSLSLLAQPAAQAAVRTGTYHEVIAKTSGMVGNVMAQ
                     GLARKHGLGILNVTWEDTGRYKGSSVGPNISDMTIQVQTKDPETGRFSLSCMPVIRHP
                     NFSDESADISPDRFFLLVGNEKSKPLRRITLKEYLSNMRKYLTDPGSWKGRAQSLLAK
                     RDTHVLVSAQACFLPVPKKGIAEFNPVLFNYQSYGDDPAVLAILATREGASATVIDNK
                     RDAFQAGRTWGQRLFFNKSGKRASLTGKRMSDFISEVGTNGGTNTTVDAAGQNGLNMV
                     LLIQVPLKQRRPMRYKMMDSAGCGMMKSPPSAARRSDVESAVIGHGTVEGPFTEIGGI
                     PIERDERFPVRVTVQFYKATSNGVISEADMREIAGQLKRVYAEGDYAGSLVVADTTGR
                     PTEFDGDKIEPPDWWESFWRRHTENTGQTPEEAIRMLKKLYGPAWQPRSEKQFTRELK
                     RAERLRRFKLL"
     gene            complement(7510..9174)
                     /locus_tag="PKJ16_01010"
     CDS             complement(7510..9174)
                     /locus_tag="PKJ16_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV45590.1"
                     /translation="MNATSTVSRYLLARLEEVGVGHLFGVPGDYVLDFLDAVVASPIG
                     WVGTCNELNAGYAADGYARMNGAGAAVVTYGVGAFSIINAVAGAFAERVPLIVISGAP
                     PARRREAGAMVHHLVADYYRQVEIFKKVTIDAAMLANPDAAPDEIDRVIRNCVTEKLP
                     VYLELPVDVALAPCRTPRPIADIAPPRSDEASLSECVAEAASLFDRSAHPLVLAGMEL
                     LRRGLGNETLRLVETAEVPFATMLGSKSVLPELHPQFVGIYQGGWSLDAVQRQVEDSD
                     CLLSLGVWMTDLDTGLFSVNLDPRRTIAAGGDQVRIGNHYYRQVQLGDFIRELAKAIT
                     PRSFLASHPAHARCPRTPFVPGKSRTLTAARLYECINAFLDDRMVLLAEPGDAFCAAP
                     EFFIEEAENFIVQSYYASIGYCTPAALGVALARPGKRPVVLTGDGAFQMTVQEVSTLI
                     RQRCPAIIVVINNDGYLIERLLHEDGPYNDIQMWRYAKLTEVFSGDGAAVGIRVETEE
                     ELNGAMKRAVDETDTLFLIEASLPGRDCSAGLDRLGRSFRQAQRKT"
     gene            9549..11369
                     /gene="typA"
                     /locus_tag="PKJ16_01015"
     CDS             9549..11369
                     /gene="typA"
                     /locus_tag="PKJ16_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HNV45591.1"
                     /translation="MNIRNIAIIAHVDHGKTTLVDKLLMQCRIFRDNQEVRECFLDSN
                     DLERERGITIFSKNISVPYRDVKINIIDTPGHADFGGEVERVLKMADGVLLLVDAFEG
                     AMPQTRFVLQKALSLHLAPIVVINKMDRPNRRPAEVLDEVFDLFIDLGANDRQLDFPV
                     IYASGRAGWAQAGPDEPGTDLIPLLDAIIAHIPAPKTNEGPLQLQVTSIDYSEYVGRI
                     AIGRVFRGEILINTAVQVIKRDGSEVKSFVKDLFIFEGLERKKTGRVSCGDICAVVGL
                     DDIEIGDTIADPDNPEPLPIIEIDEPTMSMIFTINNSPLYGQDGRYVTSRHIRERLAR
                     EMESNVAMRVEDVPSADAWKIYGRGILHLSILIENMRREGYEFQVGQPRVIYKEINGK
                     KSEPVELLVIDTPAETSGKVIEAVSAKRGELVKMESIGARTRLEFRVPARGLIGLRTR
                     ILNITSGEGVMHHRFYQYEYFKGSIPQRQNGVLVSMQEGEVVAYALDQLQSRGRFFVA
                     PGDRAYEGMIVGENNKEGDIVVNVQKSKKLTNMRASGSDDAVRLTPPIVFSLEEALEY
                     INDDELVEITPRTIRLRKMVLNETERKRQRIRKELQAVEG"
     gene            11485..11967
                     /locus_tag="PKJ16_01020"
     CDS             11485..11967
                     /locus_tag="PKJ16_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45592.1"
                     /translation="MFRAIALLAVLFAHTAAFAADPAQRTLVLGFGSKLLSDIEDRIV
                     REEVMRGLITRGHAVVPVMALEEYFLNRAGSIRAMDRAAVAGACRELSARIALSGAVT
                     RMDGRVRVALSLYRADTGETTDTAFTVDGAGEFKDYGPVLIAEILRHSERLLASRERK
                     "
     gene            11964..12824
                     /gene="argB"
                     /locus_tag="PKJ16_01025"
     CDS             11964..12824
                     /gene="argB"
                     /locus_tag="PKJ16_01025"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HNV45593.1"
                     /translation="MKKHIEKVNILTESFPYIKEFHGKTMVIKYGGNAMIQDDLKKSF
                     AMDMVLLKFVGINPVIVHGGGPQINDLLKKTGVKSEFVDGLRVTDPATMEIVEMVLVG
                     QVNKEIVNNINMVGGKAVGLSGKDGGLITAERIYHDREGAKIDIGQVGTVKRIKPSII
                     ELLDRDRFIPVIAPIGVDETGITYNINADTVAGKIAEALNAEKLILLTDIEGVKLDNE
                     LASSIPKSDIPSLIGSKQIGGGMIPKVNCCLDALSGGVHKTHIIDGRVEHAVLLEIFT
                     DAGIGTEIVH"
     gene            complement(12843..15008)
                     /locus_tag="PKJ16_01030"
     CDS             complement(12843..15008)
                     /locus_tag="PKJ16_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV45594.1"
                     /translation="MFDPRRIAIVGVTAEGFSFGRCMLLSHLSIGYEGTLYPVNRRGG
                     TTEGLTIYPSIEDIPDDIDFAVIAVPAQHVPAIVEACVKKGAAGVEILSSGFRESGTP
                     EGAALEDEVKAIAARGIRVLGPNCFGIYCPRSGQTLIPGPELSREPGGVAFLSQSGGH
                     AVDFALMGKWRGVRFSKVVSFGNGCDLRETEMLRYLTDDSETRVIGMYIEGVVDGRDF
                     FSALRGATTEKPVLVIKGGLSDSGGRAAASHTASMSGQRAVWEAALRQCNAIQLESVE
                     ELADASLAFSMIPIRPYHGCTIVGGGGALGIGAADAAESFGLAIPPLRADLREAVMDI
                     LPKPGSSATNPIDVANPFVSPSAIRQILLRASEDEAIDVHILVFLVYHFMAQRKVMGA
                     AILRDFIPGRELAAVCREVAESTGKPVVLVMPQYRQEAEAMELEAAVREVRAICIDAG
                     IPVYDDVKNALRAIAAVSRYARAREAMHGDTQNHTAPIRDAGSFDRFRGIIGAGVSRG
                     DGALNEYESKRVLREYGIPVTEERHAATLDEALAHARAIGYPVALKGSSSTLTHKTER
                     RLIELGIDGDEALVKAYRAIEERGGGMLDGMLVQQMVSGTREFVAGFMRDAQFGPCVM
                     FGLGGIYTEALRDVSFRVAPLSLRDAHEMMNEIRGASLLGAFRGMPAVDRDALARALV
                     GLGRLGLDHDEIAEIDVNPLIVRGELPVAVDALVVVRRV"
     gene            15233..15721
                     /locus_tag="PKJ16_01035"
     CDS             15233..15721
                     /locus_tag="PKJ16_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV45595.1"
                     /translation="MRILETKGPAMLSIDTLAFRTGKTKGSFYHHFNGREDYIARLLE
                     HYEKTATSEVIQLSSDAVTPRARLAALTTLTFQLSSRLELAIRAWALYDPLVRRFQDR
                     MDRRRLSYLEELHTAGGMAPDAARTRSYRDYALFLGLQQLSHHHAESEFRSLIKRLFT
                     DI"
     gene            15862..16074
                     /locus_tag="PKJ16_01040"
     CDS             15862..16074
                     /locus_tag="PKJ16_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45596.1"
                     /translation="MKAKLIAAVLALVVGIVFMAPIAKAEVEKSKCAGLKKAECKTDK
                     DCTWINKHKASDSFCKCKPGKCKPAK"
     gene            16214..16582
                     /locus_tag="PKJ16_01045"
     CDS             16214..16582
                     /locus_tag="PKJ16_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45597.1"
                     /translation="MTIARTITALCLIALIGATACKKSQPHLVLVKDTVEKSTAVVKI
                     KIAGVVKNEEKSRYWVNIVKAGSPESEWGSWIWVSDGTTELDIPMPRDVVEGAYEVRL
                     HSDYPVKTFNIIDRKPVTIK"
     gene            complement(16632..17576)
                     /locus_tag="PKJ16_01050"
     CDS             complement(16632..17576)
                     /locus_tag="PKJ16_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV45598.1"
                     /translation="MSGGVVTIDCGYLHPEFAAAYLITEGDRAAFIDNNTRFAVPRLL
                     DALAGAGRKPEKVEYIIVTHVHLDHAGGTAELARLCPNAIVLAHPRAARHIIDPSRLV
                     AGSRAVYGDETFARRYGDIEPVDATRVRSMDDGAEIALGARTLRFIHTAGHAKHHMCI
                     YDAGSNAVFTGDAFGLAYPVLQTGNEPFVFPSTSPSDFDPAEARDSIERIAGTGASVA
                     YPTHYGAVPHVARVRDMMLRSLDVMEVMLAEAIASGLDDDELERFCYRPMERFFRTEL
                     ERRGIVFSQSMQEYLADDIALNAQGIAVVALRLRGVIL"
     gene            complement(17576..17887)
                     /locus_tag="PKJ16_01055"
     CDS             complement(17576..17887)
                     /locus_tag="PKJ16_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HNV45599.1"
                     /translation="MSAVVQMSIFPLDKGPSLSGYVKDAVAEIEHSGLPHAVGPMGTV
                     IEGEWGEIAALVGRCLETMGRSSERVYLTLAVDWRRGPDGRLEGKIASLEGKLGHGVR
                     R"
     gene            17996..18781
                     /locus_tag="PKJ16_01060"
     CDS             17996..18781
                     /locus_tag="PKJ16_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNV45600.1"
                     /translation="MNDRILRAVVAIIIIVCAAFLSCANSSAADTDLYGGVRQRDYLI
                     GRYNPSAEKLFISLGTVGIPTRGRTQYLRIEAALALKQLYKHLKTDHPEISFWVQSAT
                     RTFDDQRAIWGNKWNGVTRVDGVKLNETIADPVARGLRILERSSMPGTSRHHWGTDVD
                     LNELFNGYFDRGNGATLYRWMTTHAHRYGFCQPYTAGRSAGYQEERWHWSYAPLSRAF
                     LADWMKHFGKNTDAFTAKGLFAGSEHIGGRAPLYVSSINRACE"
     gene            18790..20187
                     /locus_tag="PKJ16_01065"
     CDS             18790..20187
                     /locus_tag="PKJ16_01065"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HNV45601.1"
                     /translation="MRRHLSWPLFIALAAATCSHGEDASYSKRAPILNRLTATIGAQS
                     PVRWQSRDHTPAPVPVAAVGDPRYTARLSGGIRNIDIAAVMKQALSDGVSVILVIGDG
                     FGESHRKILATMRPTGTGRVGSHFERIANTGARGVCDTRSAGGITTDSAAAATALACG
                     VTTANGVIGRALSGARCDSALALARRRGMATGLITDTRVTDATPAAFFAHVANRRQES
                     AIAAQLVDGDTPDIILGGGACYFIPRTMAVRSHPALNGRSVGADLKSRRDDDRDLVTE
                     MAARGYRVVTNTEILISAARGSNRILGLFAGHTMNTTIDRDDENTGEPGIDLMARTAL
                     SVLARNPKGFFLMIECGQLDLVSHMNDTGAVVGALREMDDVLRVCLDHARANSARTLL
                     IATGDHETGAPVIDPQIAGMRAPALSLDAIARQRRSLRRIVIDARSTDELRTRIDTET
                     AFRINLEEAKIIWGQ"
     gene            20184..20864
                     /locus_tag="PKJ16_01070"
     CDS             20184..20864
                     /locus_tag="PKJ16_01070"
                     /inference="COORDINATES: protein motif:HMM:NF023757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-J related domain-containing protein"
                     /protein_id="HNV45602.1"
                     /translation="MSDISGDPGLALHEIDCDALGAFLESSARALMEIEVLRHVFGID
                     RMPADRRGLFAVHFSLYHALYRLKFGAGARGRYLHLDPMRIRLIAVPVTGCRWYSPEE
                     GRFCGLSTTRYDRCALHAHPTGMPDETPSFDPLQEFYLNPGNIAFGESDVLERLMNGV
                     VLYAVRRGEIEQALRLFDLSRPNRPSIDRRYHHLARVHHPDMPGGDAERMKEINRSYQ
                     VLKEVFVV"
     gene            complement(20880..21095)
                     /locus_tag="PKJ16_01075"
     CDS             complement(20880..21095)
                     /locus_tag="PKJ16_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002757627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45603.1"
                     /translation="MKKRKHVKYIHEGVYAAEIDVELLDSEEGWGPYLSLDDAVKIDT
                     VREALRNDDLTLASNYARVFTLHPAAI"
     gene            complement(21092..21415)
                     /locus_tag="PKJ16_01080"
     CDS             complement(21092..21415)
                     /locus_tag="PKJ16_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HNV45604.1"
                     /translation="MSSILKNIVFLVEQRQVFISYHGYDELSNDSISVLDVINGIKNA
                     VLIQEYPEYRKGPCVLVLQSDSEGNPIHVVWGMSKGDTSTAVLVTAYRPDPERWTDGF
                     TRRKE"
     gene            complement(21555..22211)
                     /locus_tag="PKJ16_01085"
     CDS             complement(21555..22211)
                     /locus_tag="PKJ16_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV45605.1"
                     /translation="MLEDGDKGVIIQRDKTTYAVAPHIPCGVVSPETLRRLADVAEKY
                     QAAALKLTSAARIAIVGLREEDIDAVWADLGMEPGAGVGLCVRSVKACPGTTFCKRGM
                     QDSLGVGMKLDETFHGMELPSKLKIGVSGCPNQCAETCIKDIGLVGMKNGWRLLVGGN
                     GGGKPRLSQELVRDISEAEAFAAIDRIIAFYKANGARMQRLGALIEKMGFEEFKNAVG
                     "
     gene            complement(22353..24116)
                     /locus_tag="PKJ16_01090"
     CDS             complement(22353..24116)
                     /locus_tag="PKJ16_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45606.1"
                     /translation="MIHGAEYRFYKEGLHVVNRDLPGFDGPMVRRGKLARSARFRIRG
                     TDFMFAKGSWMEFYYNGSVEKGTLDSTVRVRCGANDIVLESRTMKEKWMESIEFFGNG
                     AVSRGLLARDTRVRIGTYTATLARGNTSEHVAYFYPRGDVRIAMLAEDATLGAGNKSL
                     RFKKNTTITLYPNGSVMRGTLAEAVPPEASGVSMEIPAGSLVELYADGKVASVFTGAS
                     ATVSMQVVRFTAPAQSWFSFFRSGAVQSASVKEPLDVTVQGHTIFKNRYGRAELHENG
                     RIAMVSFSVSSRHRAGGRMISCAPGSVSFHANGSLMEAEIAEPISAIMNGVSLTFMKG
                     RVRFYDTGAIESGMIEGTGYIDRLTERYFPGFSTNVEKRKAQGFHPGKYRYSFHRNGA
                     VRSAYIENSWAIITSRIMGGAVMPDSGNIGFHADRRPSFFTLREDALLSLRGKPSRIP
                     QSARLVLAPSSEVFSIYTDRDAHCLMGGKPTGPHIGAYVVFHDYEKKIVDALAFRGID
                     DLFGWNQRPDRLVFSNIVIELEPLVAYDENDVLDTHIWVRYDDFHSATVRSLLFARER
                     RGFVDGREVRFPAFRWVDVGG"
     gene            complement(24406..24576)
                     /locus_tag="PKJ16_01095"
     CDS             complement(24406..24576)
                     /locus_tag="PKJ16_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45607.1"
                     /translation="MKIEFSEQEAAFIFFMMENMTKFEGLDKQTQSAVHALVARIQAT
                     LRAHGIDERSRF"
     gene            complement(24685..25152)
                     /locus_tag="PKJ16_01100"
     CDS             complement(24685..25152)
                     /locus_tag="PKJ16_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HNV45608.1"
                     /translation="METIIVTSSAFRNGGTIPARFTCDGPDVSPPLSWTGVPAGAKGL
                     ALICDDPDAPAGDWVHWVMYDIPPSTTALAENQPTTQTLPGGAKQGVNDFRKIGYGGP
                     CPPGGTHRYFFTIYTLDIALNLEPGKTKAELLRALQGHIVAQGQLMGAYTRTR"
     gene            complement(25355..25756)
                     /locus_tag="PKJ16_01105"
     CDS             complement(25355..25756)
                     /locus_tag="PKJ16_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45609.1"
                     /translation="MNIKKYLLASVAVYVAFQAIDFVFHGIIMADTYKALSHLWRSDM
                     MSYMWIMYLAGVVLAGAFTYIFVKGYEGKGLLEGVRFGIVAGLFMNVMGMFGQYAMYP
                     VPFSLTLIWFGYGMAQYVIGGVIAAAIYRPE"
     gene            25997..28333
                     /locus_tag="PKJ16_01110"
     CDS             25997..28333
                     /locus_tag="PKJ16_01110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008340443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV45610.1"
                     /translation="MNIDQLIEYLQSYQSFSENVRLWMRVDAFDGTYTDYPDGVEPNL
                     VARYRTRGIERLYAHQREAFDRVRAGDNVVVVTPTASGKTLCYNLPVIDAIAKNPEAR
                     ALYLFPTKALSQDQLAELNAIVEDTGIELRTYTFDGDTPASARKAVKAAGNIVITNPD
                     MLHAGILPHHTIWIKLFENLKYIVIDEIHSYSGVFGSHFANLMRRLHRICAFYGSRPQ
                     FICCSATIRNPKEHAEALIGRECALVNNNGAPRGERHYVIYNPPVVNEQLGIRASAVK
                     EAAKVGSFVLLNKIPTIVFARSRMRVEIVSTYLRHKCPGVSIVGYRGGYLPTERRRIE
                     QGLRAGSITGVVSTNALELGLDIGMLDVAITVGYPGSISSLHQQFGRAGRRGNKSLAI
                     MVATSSPLDQYIANNPDYIVKSNPESATINPDNLLILMDHLKCAAFELPFHADERFSA
                     HLGSTRDMLDYLAERGVLKRAEDTYHWMSDIYPANEISLRTAAQENFLIINDKQDKTL
                     RAMPDPAGVSRRDPAARRNPKVIGEVDYYSAATLIHEDAIYIHQGRQFFIDTLDWEGR
                     MAFCHEVESDYYTDAETKTDIHVLEKFEERSLPIAVFSRGEITVRTTPVMFKKIKFDT
                     HENLGWGKIHLPEIEMHTAAAWLDIDEERLHAMAGREIAGRVLYGAAYLLRNIAPIYT
                     LSDVSDVRVQHQARSNYSGKPAIFIYDSTPGGVGLSERVYAILDVIAAEAVRAIEVCE
                     CRYGCPGCVGPLPANDEHIKRLTAAFLRECCGEHQGQA"
     gene            28311..29537
                     /locus_tag="PKJ16_01115"
     CDS             28311..29537
                     /locus_tag="PKJ16_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HNV45611.1"
                     /translation="MNIRDKLKLYETTAPAQSTPQPATMPESLARAGGEVVTSNGETL
                     WRFTERVPLDAIVPASADDGTVCPLRPYLRYNRHPDDLSLDTLVFLDLETTSLSIGTG
                     NYPFVIGLGFVDGGSFVVEQYLLHDYRAESFLLAHIVPRLRSARALVTFNGKSFDVPL
                     LKNRYRLSGVPGYPVDTPVIDLLFPCRRIFKSIYASCSLKSMEEKFLGIVRNDDIPGW
                     MIPDVFFEFQRTGAIDCIPAVLLHNAIDVRSMASLLFALNDIYRGVADKNFTALETRA
                     LMNIAHGLYRVDRALFIDLVEFIGREVFGDRRLFKKYGAALKRTGQHDRAIELWREER
                     SLYSLEELAKHFEHRARDYGHALAQCREAAVLIDAGVLRVGGDPAPAGIITWYAERFA
                     KREKRLHRKAGDAEAT"
     gene            29521..30294
                     /locus_tag="PKJ16_01120"
     CDS             29521..30294
                     /locus_tag="PKJ16_01120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV45612.1"
                     /translation="MPKRRKTADTGPGAATTEVYTEFARFYDTVLKHVDYDRWYRYIK
                     SIMLLYVEHPNRLLELGCGTGRFGAKFSRDRYVIFGMDRSLEMLRVSRMRAYRNFRVL
                     CGDMRQLPLAGTFDFIFSVHDTMNYFLEYDDIRRVLRSVRRAMADEGVFMFDITTEHN
                     INRHFDNTVMRHSVRGTEIEWRNYFDRDTRLVYSVLDFRDRRGVVRTEKHVQRIYETD
                     EILTLLEEEGFMTLDVFSDYSFEPVSTTTVMTNFVTRKK"
     gene            30296..30649
                     /locus_tag="PKJ16_01125"
     CDS             30296..30649
                     /locus_tag="PKJ16_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45613.1"
                     /translation="MEYVFYAIGLLVGVLAFLYMVSLYEAGKSKVREIITPDRERGAN
                     AVQVNPSAVTVNNAVGRHPGERICPLCGSVLTKYEALYATFIDTEGDRRLLIHGCRYC
                     HRPDEKPEEKKKSDL"
     gene            complement(30650..31363)
                     /locus_tag="PKJ16_01130"
     CDS             complement(30650..31363)
                     /locus_tag="PKJ16_01130"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HNV45614.1"
                     /translation="MATKNDFISGLKKHKIKKPVLDAFEAVNRAKFFDPFFKNKLYLP
                     DEPIPIGYGEMSDPLLSLLQMLNHLAPKRSARVLEVGTGSGYSTALLAHLVDEVATVE
                     HNEKLALGAKDTLAANKIDNVRIFVGDGTDPRSNLGIFDAIIIFGACKIRPLRLIQCL
                     KKGGSMVFPMGPIIRQQIALVVNDLMVDDVQLYKTKFHEFCVFPPLRGMFGTDFEGFP
                     NLSEEVLPGVEKPSGKEQP"
     gene            complement(31376..32713)
                     /gene="dnaB"
                     /locus_tag="PKJ16_01135"
     CDS             complement(31376..32713)
                     /gene="dnaB"
                     /locus_tag="PKJ16_01135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HNV45615.1"
                     /translation="MTVDKLPPQDIEAEVSCLASILLSKAALLKVLEILHPEDFYLER
                     HKYIFEAITELERKNLPIDLVMLKQRLSDANLFERVGGDEALVAIYQAVSTSANAEFY
                     ARRVKELSLRRRLIEVSTDVVEKCFNMGRDTEELLDEVERDIFTVTERRITTDLRSIA
                     DILPETLNAIGQMYETKRAVTGVASGFTEMDETLTGFHESELIIIAARPSMGKTAFAL
                     NIANHVALRDRKPVLFFSLEMPATQLATRMLCIEAMIDSQRVRTGHISHEELNALVQT
                     SGRLGKSPIFIDDSPAVNILELRAKARRRAQKGELGVIIVDYLQLISSLSRTERHLQI
                     AEISRSLKQISRELNVPVIALSQLSRAVEARTDQRPQLSDLRESGAIEQDADVVMFIY
                     REERVKKDTEKKGIAEIIIAKQRNGPIRNVELKFWEKYTKFDNLDRIHGAEEY"
     gene            complement(32717..33163)
                     /gene="rplI"
                     /locus_tag="PKJ16_01140"
     CDS             complement(32717..33163)
                     /gene="rplI"
                     /locus_tag="PKJ16_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HNV45616.1"
                     /translation="MKVILQKDIPNLGDAGDVKDVSPGYARNFLLPKKLVLVANESSQ
                     RAIEHQNKLIKIKKDKRKKQSEKLIESLSGVSVTISAQVGEEGKLFGSVTSIDIARKL
                     KEIGHEIDKRKIQLADPIKQVGEYEVTLKLEEGLNATVKVIVEQAV"
     gene            complement(33228..34103)
                     /locus_tag="PKJ16_01145"
     CDS             complement(33228..34103)
                     /locus_tag="PKJ16_01145"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HNV45617.1"
                     /translation="MALVAFILLSVALLVGSAYASARWGWIPPAASMAAAVALAAIVG
                     ADVAKLAEVAAPVVIGSAAGCSLKYGRSKQFFLIVSACVLAALFSATYYSLTLHRNID
                     VLAESKRVFKEMVASSGMAESDKKEFFERFDEYAAVAVETIPFVHFLYGLLLSAVGVV
                     IIRMALKRVIGDTDALSQGLEYFALNDYAIFALIATWLVVLLVDSKEQLVIHRIGLNG
                     ALIVSMLYVLQALGVIKFLLLKRNVPVFLLPLTLGIVAVAGAPFLLFVLVILVSLGSL
                     DVWVDFRKLRPSQAP"
     gene            complement(34105..34449)
                     /gene="rpsR"
                     /locus_tag="PKJ16_01150"
     CDS             complement(34105..34449)
                     /gene="rpsR"
                     /locus_tag="PKJ16_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HNV45618.1"
                     /translation="MSDEHIAEPAQQPEAPVEATETKAPEQREARRPYREGGPRGQGG
                     QKFKKKRCRFCFNKDIKIDYKNNELLERFITDRGKILPRRITGTCSKHQRELSTAIKR
                     ARIIALVPFIVQ"
     gene            complement(34492..34926)
                     /locus_tag="PKJ16_01155"
     CDS             complement(34492..34926)
                     /locus_tag="PKJ16_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV45619.1"
                     /translation="MASDLNRIILIGRFVKDPELRYTQNGKSVTNFSVANNRSYTVGT
                     DKKEYTSFFSCIAWGKLGELIVQYCKKGNRIAIEGRLQQRSWDDQDGKRRSAVEIVVE
                     NFQFLTSKQDKDAMTPDIPGPSDMGPGSNIDDHPFSDNEVPF"
     gene            complement(34945..35235)
                     /gene="rpsF"
                     /locus_tag="PKJ16_01160"
     CDS             complement(34945..35235)
                     /gene="rpsF"
                     /locus_tag="PKJ16_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HNV45620.1"
                     /translation="MRTYELTVIVRINSDLESNIAKVKDILQKHGALIENDQSWGSRR
                     MAYEINGEREAHYLLLLIQTPADSVAKIIAEFKLNQNILRYLFVVMPQKKTA"
     gene            35392..36024
                     /locus_tag="PKJ16_01165"
     CDS             35392..36024
                     /locus_tag="PKJ16_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45621.1"
                     /translation="MKKILSTHDLKPGTVLRVYKKGFGYAILRVIEAHELYLAAFAPD
                     DFISRAVPGDRLKAYLWIEESGTYEFPLVVNGSISRQHAILFCGHTDRIKRGDAPKCL
                     TARLSAPLRFFVLDANSSKGFTTKRIVVHTGALVELSDRSALLRYDGDLSDGTLIKGH
                     LRSGSHTVDILGRVTRSATRGARRYRIDISAMNERERNAVLDYVFSVYRE"
     gene            complement(36030..36479)
                     /locus_tag="PKJ16_01170"
     CDS             complement(36030..36479)
                     /locus_tag="PKJ16_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45622.1"
                     /translation="MSPRARAILVVLSLLLAAAPARQGLPAGGAGPIEHREFFERLAS
                     LPPIMRDGFLDEQVNRLLNGTGHVVSVDELSRYNRGVRIIATESATSSMNITYYIFTD
                     QRSFLTSLKKNDAFAFSGLFKVYTPLSSRRDSYLFDVVLVEGAPVRR"
     gene            complement(36485..>37169)
                     /locus_tag="PKJ16_01175"
     CDS             complement(36485..>37169)
                     /locus_tag="PKJ16_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV45623.1"
                     /translation="VGMNTAIRTVSGGYQGISFAIPSNIIRQVCNDLIQHGRVRRGWL
                     GFIAREIAPGKRPARKQLEVITVIKNSPAEHGGIRRGDRIDEINGKRISSLGELVTNV
                     GNAPPGSMIAVTVTRDGRRHTIRLVSREKGDDARASRGADDLYARYGIELSENARTGD
                     VVVSALSPMGLGYRNGLKRGDIVVSLNAADVATLEDVAGVMRRSDGRVEKIGIYRGSR
                     RYTIPLDGN"
BASE COUNT         7383 a        11893 c        10867 g         7026 t
ORIGIN      
        1 catcgaagag cttggcgaag ggcccggtga agtcgaaaaa ctggttcacg atgatgaggt
       61 gcccggtgga cgagatcggc aggaattcgg tgatcccctc cacgacgccc aggatgaccg
      121 caacgatata ttcgctcata cacgcactcc gcagggaatg atgacgcacg cgccacgcgg
      181 cgccgcgcgc cgcgaatcag ccctcactag cggcgcgccc cgtttcgcgc aagggatttt
      241 ttcgtccgcg gaatcagccc ttgaccatca ccaacagcat cttgaaggcc gacaccgccc
      301 gaagcgcatg cggttttccg gccggcatga tgatcatctc gcccgcggaa accgaatgcg
      361 cgctcccggc gatggtgatc tcgaccgttc cctcgaccac gtacacgaag gcgtcgaagg
      421 gtgccgtgtg ctcgctcagt ccctggccgg catcgaacgc aaacagcgtg atggtccccg
      481 cattcttgtt gatgagcgtt ttgctcacca ccgcgtcctg ctggacgtca atcgcccccg
      541 ccagcgcgat cccgccgcgc gcgtactcct cgattgttcc cgcatgtgtt cccatggcat
      601 tctcctatcg aaaaaaatga agcagcagat cgatcctccc gcgacggacg agatgcatcc
      661 cggagaagcg catcaccgcg cggatccgct cgcggtgccc gggcgcatag caatgcgtgg
      721 ggcagcgctt gcagttcggc ttcggatcgt acgggcagat gaggcgcttg ctcgcggcgt
      781 gcagcaaaag cgccgcgcat tcgccgcaca gcgcgaccgc gtgtcgattg acgagcgtcc
      841 ccaccgcccc gcccgcctgc actcgcttct tcttctgttc cgcatgtacg cgcccgcaat
      901 agatcccgat gaaattcgcc agcgtcccaa tgtctcgttc gagtacgtct gatgatcgtt
      961 tcataagcaa taaccaataa taattaggaa tcctaataaa atgcactcta tcgcacatac
     1021 ccccgttctg tcaaataaat taccggtagg gtatttttac ttgcgtttgg ccttcgtcga
     1081 tcgcaccatg cagccgcaaa acgtgtcgag gcatcatgca gaaatcacat ccctccaaca
     1141 gacctgattt gacgatccag tcgctgcgcg ccggcgaacc gatcaccatc gtcgccatcg
     1201 gcgattcgct caccaacggc tggatggtgc gcaagggcta tctcgatttc ctcggcgaga
     1261 tgctccgcga gacgtatccg aaggcgcaat ttaaaatcat caaccggggc attccgggag
     1321 acaccgcgga cggcggcctc gaccgcctgc ggggcgacgt gatcgatttc gaccccgatt
     1381 gcgtgctggt ccagttcgcc ctgaacgaca tgttcatggg ctacccggcc gcgcagtaca
     1441 agaactacct gcaggccatc atcgattcgc tcatcaacga tacctccgcc gagaccgtgc
     1501 tcgtcacgtc ggtctgtctg ggcgacacac gcgaaaacga acgcgccaac gagttctatg
     1561 aaaagatgga ggagctcgcg cgtgagaacg gcgtgtccat cgcgcgggtg catgcgcact
     1621 gggcgcgcgc gatcgccgat ggcgccgcct tccgaacgct cgtgcaatcc gacatggtgc
     1681 atcccacggt ggaaggctac cggctcatgg ccgaggcgat catgcaggtg tttcggtaac
     1741 gcgcgcggcc actcgggtga tcgcgcaccg ccgtcgaagc aatcacggag gtttacaatg
     1801 aaaccattga tcggaagcgc cctcgccgcc gttttcctca tgacgatggc gcccctgctc
     1861 catgccgatg gcgaactctc ctccgcggag ctacagggcg tcccgcttga aagcggccgc
     1921 acgatcccga ggctgcgcat cgcttatcgc acctacggcg cgctcaacgc cgataaaact
     1981 aacgcggtgc tgttccccac ctggctcatg gggacctcga aggagctcgc cgatctcggc
     2041 tacatcggcc ccgggaaggc ggcggacagt tcgcggtggt tcgtcatcgc cgtggacaat
     2101 cccggcaacg gcgtttccac ctcgccctca aatcgcgcgc gccacggcgt caccgagctt
     2161 cccccgatga ccgtgcgcga cctggtgaat gtgcagcgcg cgctcgtcac cgggcacctg
     2221 aagctcgaac ggctgcatgc ggtgatgggg atctccatgg gcgccctgca ggcgctcgag
     2281 tggtcggtct cccatccgga catgatgacg cgcgtcgtcg cgatcgcggg gaccccgcga
     2341 atcaccacgc acgagctgtt gctgtggaac tcgcaggtgg ccctcatcga ggccgcgcgc
     2401 ggcatgaagg acggcaatcg gaccgccatg cggctcatcg ccccgaccca tgcgctcatg
     2461 tcgcgcacac cggaattttt catcacgcag gtgccgccgg agaactatcc ggccctgctg
     2521 aatgccacgg aaacggcgct cgccgcctat cgcgcggagg actgggcgtg gcaggtgcgc
     2581 gcgattgtga cccacgatat cgcgcgaccg tacggcggat cgctcgcgcg cgccgcggcg
     2641 cgggtgcggg cacggacgct tgtcatcgta tcgccgggcg accgcatggt gcttccccaa
     2701 ggcgcgctcg agtatgcgcg cctggtcagg gccaaaacga tcgagaacac cggcgcatgc
     2761 gggcatttct cgtttctgtg cgaacgggac gcgatcgccc cgcggatcag aacattcatc
     2821 gaataatgcc aggctgcgcc gcgagagtca ctcccacggc agcttctcgc ggggaacggt
     2881 gttgatccac atgaagcgtt cgaggtatgc ggcaagcatc cggcggattt cttccggggt
     2941 cagagccccc tcgaccgcgg gagactctgc gtaatgcgat gcggaatcgt gatccgaggc
     3001 caccattcca tcatcccttc cgatacacga ccctcccccc gcagatcgtc cactccaccg
     3061 agccgtagag ctcgcgtccc aggaagggcg tgttcttgca cttcgaggcg atgaaggcct
     3121 gatcgatcag gacctttttt tccggatcga tcacggtgat atcggcggcg gcgccctcct
     3181 gcagggcgcc gcgatcgagc ttgaggatgc gcgccgggtt gatggtgacc ttctcgaagg
     3241 cctgcaggag cgaaaagccg ttctcgcgca ccaacacggt gacgatgagc ggaacggccg
     3301 tctccaggcc gatgatgccg aagggcgcgt actcgatctc ctgcatcttc tcgttgatgg
     3361 ggtgcggcgc gtggtcggtc gcgatcacgt ccacgctgcc gtcgcgcagg cccgcgatga
     3421 tcgcctgccg gtcctcctcg gtcctgagcg gcgggttcat cttcgccatg gagaggtgct
     3481 gctcgaccgc gtcgtcggtg agcgcgaagt agtgcggcgc cgtctcgcag gtcacgcgca
     3541 ccgcgtcgct cttggcgcgc cggatgatct cgaccgaccc gccggaggag acgtgcgcta
     3601 cgtggacgcg ccccttggcg agccgcgcga ggatcatatc gcgcgcgatc atcacctcct
     3661 ccgccgcctt cgggatgccc ggcaggccca gcagtactga gttggcgccc tcgttcatga
     3721 tcccgccgtt cgcgagctgc gcgtcctccg aatgggtgat gatggggacg tcgaacatgc
     3781 gcgcatactc gagcgcgcgg cgcatcacga tggagctcat cacgggcttg ccgtcgtcgg
     3841 agaagcccac ggcccccacc ttcacgagct cgcccatctc gctgatctcc tcgccctgcc
     3901 cgcccttggt gatggtggcg atggggaaga tgttgatggg gccctgctcc gcctcgagct
     3961 tgatgaagcg caccagggcc tggttgtcga tcggcgggac ggtgttgggc atggtgcaca
     4021 cggaggtgaa gccccccttc gccgcgaccg tcgacccgcc gatgatcgtc tcgatgtcct
     4081 cgcggccggg ctcgcggaag tgcacgtgca tgtcgataag gcccggcagg acgacgcacc
     4141 cggcggcgtc gatggtctcg tcgacgctgt cggcgatgtt cgccgcgatc ttcgcgatga
     4201 agccgtcctc cacgagaacg tcgcgccggt cgtcggtatg cgaggccggg tcgatcaccc
     4261 ggccgttttt tatcacaagt ttcatatcat tccccgatgc cctccagcgg cgcgccgccc
     4321 gccagcaggt agaagatcga catccgcacc gccacgccgt tggtcacctg ctcctctatg
     4381 atggcgttgg cgctgttggc cgcgccgtcg tcgatctcga tcccgcggtt gatgggcccc
     4441 gggtgcatca cgatgatgtc cggcttgcag cgccgcaacc gctcgttggt gagcgcgaac
     4501 tgacggtggt actcgcggat ggaggggaag tgcgatccct tctggcgctc cttctggatg
     4561 cgcagcatgt tgatgacgtc cagcttcccg atcaggccgt cgaggtcgta ggtgatgtcc
     4621 acgccgtatt ggcgaaactc gtcgggcacc agcgtgggcg gaccgcagag cgtgaccttc
     4681 gcgcccagct tgttgaacac ctcgatgtcg gagcgcgcca cgcgcgagtg cttgatgtcg
     4741 cccacgatgc cgatgtgcag gccggcgagc gacccgcgct tctcgagaat ggaataggcg
     4801 tccagcatcg cctgcgtggg gtgcgcgtgg aagccgtcgc cggcgttgat gaccgaggcg
     4861 ccgatatttt tagataaaaa atgcggtgcc agtgacaccg catgccgcat gacgatgtaa
     4921 tcggccttca tggcctcgag cgtatgcacc gtatcgatga gactctcccc cttgaccacg
     4981 ctggaggtgg acaccgcgat gttgatgagg tccgccgaga ggcgcttggc ggccagctcg
     5041 aaggagagct tggttctcgt ggacggctcg tagaagaggc agcagaccgt cttcccgcga
     5101 aggaccggca cggtcttcac cgagcgcgta aagaggtcct tgaagtaccg ggcattgttg
     5161 cagatgagat taatttcgtc gagggagagg tcttggatgt cgagaaggtc ttttcgattg
     5221 aggccggtca tcttgaaagc cgtagtattg gcacgatcga ggtcgtgtca aatgaaatat
     5281 tgcaccttaa attatttttt accgcccacc cgtgccgcat tgcgggcatg cgcgcagcgc
     5341 ctctcattgc ggcaggttcg catcgccccg cgaaaaccgt caattttttt attcaaccat
     5401 ggaacgagtg ccccgccata tcggtcgaag gaccaggtgg ctatcatacc aaaaatgaag
     5461 gagaatgcaa tgctacgact cattatgata ctatccctga gtttgttggc acaaccggcc
     5521 gcgcaggccg ccgttcgcac gggaacctac cacgaggtta tcgcaaaaac gtccggcatg
     5581 gtgggaaacg tcatggcgca gggactcgcc cggaagcacg gcctggggat cctgaacgtg
     5641 acctgggagg acactggccg atacaagggc tcgagcgttg gcccgaacat cagcgacatg
     5701 acgatacagg tccagacgaa ggacccggaa accgggcgat tcagcctcag ctgcatgccg
     5761 gtgatccgcc atcccaactt ttccgacgag agcgccgaca tcagtccgga ccgtttcttc
     5821 ctgctggtgg gaaacgaaaa gagcaagccg cttcgccgga tcacgctcaa ggaatatctg
     5881 tccaatatgc gaaagtatct taccgatccc ggctcgtgga agggacgcgc acaatcgctt
     5941 ctcgccaagc gcgatacgca tgtgctggtg agcgcgcagg cctgcttcct gccggtcccg
     6001 aagaagggga tcgcggagtt caacccggtg ctcttcaact accagtccta cggcgacgat
     6061 ccggcggtgc tcgccatact cgccacgcgg gagggcgcca gcgccaccgt catcgacaac
     6121 aaacgggacg ccttccaggc gggacgcacc tggggccaac gattgttttt caacaagagc
     6181 gggaaacgcg cttcgttgac cggaaaacgg atgagcgact tcatctccga agttggaacg
     6241 aacggcggca cgaacaccac cgtcgacgcg gcgggacaga acggactgaa catggtgctc
     6301 ctgatacaag ttcccctcaa gcagcgtcgt ccgatgcgtt acaagatgat ggactcggcc
     6361 ggctgcggca tgatgaagtc gccgccctcc gccgcgagac gaagcgacgt cgaatcggcg
     6421 gtcatcggcc atggaacggt cgaggggccc ttcaccgaga tcggcggcat cccgatcgag
     6481 cgcgacgagc gcttccccgt tcgcgtcacg gtgcagttct acaaggccac cagcaacggc
     6541 gtgatctccg aggccgacat gcgggagatt gccggccaac tgaagcgcgt ctatgcggag
     6601 ggcgactatg cgggaagcct ggtggttgcc gataccaccg gacggcccac ggaattcgac
     6661 ggcgacaaga tcgagccgcc cgactggtgg gaatcgttct ggagacgcca tacggaaaac
     6721 accggccaga cgccggaaga ggcgatcagg atgctcaaga agctctacgg gcccgcatgg
     6781 cagccgcgca gcgagaagca atttacccgc gagctgaagc gagcagagcg attgcgcaga
     6841 ttcaagctat tgtaaatcaa tcagatcgga taacccgggc tgctgccata cacaccctat
     6901 tatctgggtt tgaaaaatgg ttatacatcg tcgggtactc aactcgtatg gtctgcagtt
     6961 cagaatgggg agtcccccgc ttggcttcga tacgccttcg gctactcagc cagcggggac
     7021 tgcttcctcg tcagcccgct cctcatcttg ttcgtttgca aaatacctgc agtccttccg
     7081 gaacagctta gtgactgaac agctaacagc ctaccctaat agctttgtgg ctgagtaact
     7141 atcaaccgac tcgaattgcc tggtagctga gtaactaacg gcctaccgta ttagcccggt
     7201 ggctgagcag ctaacggcct accgtattag cccggtggct gagcagctaa cggcctaccg
     7261 tattagcccg gtggctgagt agccgaaggc gtatcgaagc caccgtttca ctcgaatgta
     7321 catttaccaa tgttccatcc acgcaagtct tccgtgctcg gcggatggta ggctgtaaat
     7381 tttttacccg gtttcagaca ctttttggtt tggaagaaga aacgtctgca cggtcaaccc
     7441 agttcaatcg gtagtatgtc tcctgattcc ttacaaaaaa ggccgcacgt tcattgatgc
     7501 cgaacccact cacgttttcc gctgtgcctg gcgaaacgat ctgccgaggc ggtccaggcc
     7561 ggccgagcaa tcgcgcccgg gcaggctggc ctcgatgaga aaaagtgtat ccgtctcatc
     7621 gacggcgcgt ttcatcgccc cgttcaattc ctcctcagtt tcgacgcgga tccccacggc
     7681 ggcgccgtcg ccggagaaca cctcggtcag cttcgcatac cgccacatct ggatgtcgtt
     7741 atacggcccg tcctcgtgga ggaggcgctc gatcagatag ccgtcattat tgatgacgac
     7801 gataattgcg ggacaccgct gacggatcag cgtggagact tcctgtaccg tcatctgaaa
     7861 ggcaccgtcg ccggtgagga cgaccggccg cttcccggga cgcgccaggg ccacgcccaa
     7921 cgcagccggc gtacagtagc cgatggacgc gtaatagctc tggacgatga agttctcggc
     7981 ctcctcgatg aagaactccg gcgcggcgca gaaggcgtcc ccgggctccg cgaggaggac
     8041 catgcgatcg tcgagaaatg cgttgatgca ttcatacaat cgcgcggcgg tcaacgtgcg
     8101 tgattttccg ggaacgaacg gtgtgcgcgg acaacgggcg tgggcaggat gcgacgccaa
     8161 aaacgagcgc ggtgtgatcg ccttcgccag ctcccgaatg aaatcgccga gctgcacctg
     8221 ccggtaatag tggttgccaa tgcgaacctg gtccccgccg gcggcgatcg tccgccgcgg
     8281 atcgagattc acgctgaaca aacccgtgtc gagatcggtc atccatacgc cgagcgacag
     8341 aagacaatcg gaatcctcca cctgtcgttg tacggcatcg aggctccatc ccccctggta
     8401 gatccccacg aactgcggat gcagctccgg taaaacggac ttgctgccca gcatggtggc
     8461 gaacgggacc tccgcggtct cgaccagtcg aagggtctcg ttccccagtc ctcgtcggag
     8521 caattccatg ccggcaagca ccagcggatg ggcggagcga tcgaacaacg atgccgcctc
     8581 ggcgacgcac tccgataggc tggcctcgtc ggaacgtggc ggggcgatat ccgcaatcgg
     8641 ccggggagtg cgacaggggg cgagcgcgac atcgacgggc agctcgaggt agacggggag
     8701 cttttcggta acgcaattcc gtatcacccg atcgatctcg tcgggagcgg cgtcggggtt
     8761 cgccagcatg gcggcatcga tggttacttt tttgaatatt tccacctgcc ggtagtaatc
     8821 ggcgacgaga tgatggacca tggcgccggc ctcccgtcgg cgggccgggg gagcgccgct
     8881 gatgacgatc aaaggaacgc gctcggcgaa cgccccggcc acggcattga tgatgctaaa
     8941 ggccccgacc ccgtaggtga cgaccgcggc gcccgcgccg ttcatccgcg catagccgtc
     9001 cgccgcgtat ccggcattga gctcgttgca ggtgcccacc cagccgatcg gactggccac
     9061 gaccgcatcg agaaaatcca gaacataatc gccgggaact ccgaacagat gcccaacgcc
     9121 cacctcttcc aggcgcgcga gtagatagcg ggaaaccgtg cttgtcgcat tcattgcgag
     9181 ccccccttga tttcgcgtgt gcggcatagt ggcgtccggc gaacgtacca gatttacgcg
     9241 ccagaaacac ccgttaacgc cctgcaatgc atccctcccg gcaaaggagg ccacacgaaa
     9301 aacaaaacgt caagtgatat acggtagcga tgatagagca tcgctctcaa cagtccttcc
     9361 atgcacacac atccgccgca ccggaatcgt tcagcgataa tccattagaa gaaaacctgc
     9421 tcgccacatt caaagaaaga tcattgacga tttaccccca ccgctgtttt tcgccctatc
     9481 gacatcacaa accgccggag gcgcactggc gcgcgttccg gcgctcccag caaggaccga
     9541 tacgaacaat gaacatccgc aacatcgcca tcatcgccca cgtggaccac ggaaagacca
     9601 cgctggtgga caagctcctc atgcagtgcc ggatcttccg cgacaaccag gaggtgcgcg
     9661 agtgcttcct ggactcgaac gacctcgagc gcgagcgcgg catcaccatc ttctcgaaga
     9721 atatctccgt gccctaccgg gacgtgaaga tcaacatcat cgacacgccg ggacacgccg
     9781 atttcggcgg cgaggtcgag cgggtgctca agatggctga cggcgtgctc ttgctggtgg
     9841 acgccttcga gggagccatg ccgcagacgc gcttcgtgct gcagaaggcg ctctcgctgc
     9901 acctcgcgcc catcgtggtc atcaacaaga tggaccggcc gaaccgccgg ccggccgagg
     9961 tgctcgacga ggtgttcgat ctcttcatcg atcttggcgc gaacgaccgg cagctcgatt
    10021 ttccggtcat ctacgcctcc ggccgcgcgg gctgggccca ggccggcccc gacgaaccgg
    10081 ggaccgacct gatccccctc ctcgacgcca tcatcgcgca catccccgcg ccgaagacga
    10141 acgaggggcc gctgcaattg caggtcacca gcatcgacta ctccgagtat gtggggcgca
    10201 tcgcgatcgg tcgcgtgttt cgcggcgaga tcctcatcaa caccgccgtt caggtgatca
    10261 agcgcgacgg gagcgaggtg aaatccttcg tgaaagacct cttcatcttc gaggggctcg
    10321 agcggaaaaa aaccggacgc gtctcctgcg gcgacatctg cgcggtggtg ggccttgacg
    10381 acatcgagat cggcgacacc atcgccgacc ccgacaatcc cgagccgctc cccatcatcg
    10441 agatcgacga gcccacgatg agcatgatct tcaccatcaa caactcgccg ctctacgggc
    10501 aggatggtcg ctacgtgacc agccgccaca tccgcgagcg gctcgcgcgc gagatggaga
    10561 gcaatgtcgc gatgcgggtg gaggacgtcc cctccgccga cgcgtggaaa atctacggcc
    10621 gcggcatcct gcacctgtcc atcctcatcg agaacatgcg gcgcgaggga tacgaattcc
    10681 aggtggggca gccgcgcgtc atctacaagg aaatcaacgg caagaagtca gagccggtcg
    10741 agctgctggt gatcgacacc ccggccgaaa ccagcggcaa ggtgatcgag gccgtgagcg
    10801 ccaagcgcgg cgagcttgta aaaatggagt ccatcggcgc gcggacgcgc cttgagttcc
    10861 gcgtcccggc gcgcggcctc atcggcctgc gcacccggat cctgaacatc acctcgggcg
    10921 agggcgtgat gcaccaccgc ttctatcaat acgaatattt caagggctcc atcccccagc
    10981 gtcaaaacgg cgtcctggtt tccatgcagg agggggaggt ggttgcgtac gcgctggacc
    11041 agctccagtc gcgcggccgg ttcttcgtcg cgcccggcga ccgcgcctac gagggcatga
    11101 tcgttggcga gaacaacaaa gagggcgaca ttgtcgtgaa cgtacaaaag tcgaagaagc
    11161 tcacgaacat gcgcgcttcc ggcagcgacg acgccgtccg cctgacgccc ccgatcgtct
    11221 tctccctgga ggaggcgctc gagtacatca acgacgacga gctcgtcgag ataacgcccc
    11281 gcacgatccg cctgcgcaag atggtgttga acgaaaccga gcgcaagcga cagcgcatcc
    11341 ggaaggagct tcaggcggtc gagggatgac cgttccgcct gcggatttcg attgacaagg
    11401 gcggcgcgat ccggcacggt ttttcaccga atgcccgtcg cacggcgctg cactcgtccg
    11461 accgcgttca cggaggccac cagcatgttt cgcgccattg cgctcctcgc cgttcttttt
    11521 gcgcacaccg ccgcgtttgc ggccgacccc gcgcaacgga ccctggtgct gggatttggc
    11581 tcaaagctcc tgtcggacat cgaggaccgc atcgtccgag aggaagtgat gcgcggactc
    11641 atcacacgcg gacacgcggt cgttcccgtc atggcgctcg aggaatactt cctgaaccgc
    11701 gccggctcga ttcgcgccat ggaccgcgcg gccgtggcgg gcgcgtgccg cgagctttcc
    11761 gcccgcatcg cgctcagcgg cgccgtcacc cgcatggacg gccgcgtgcg ggtcgcgctc
    11821 tccctttacc gcgctgatac gggcgaaacg acggacaccg catttaccgt tgacggcgcg
    11881 ggcgaattca aggactacgg acccgtcctc atcgccgaga tcctccggca cagcgaacga
    11941 ttactggcat cgagagaacg aaaatgaaaa agcacatcga gaaggtcaac atcctcaccg
    12001 agtcgttccc ctacataaag gaattccacg ggaagaccat ggtgatcaag tacggcggca
    12061 acgccatgat ccaggacgac ctcaagaagt ccttcgcgat ggacatggtg ctcctgaagt
    12121 tcgtgggcat caatcccgtc atcgtccacg gcgggggacc gcagatcaac gatctgctca
    12181 agaagaccgg cgtgaaatcg gaattcgtcg acgggctgcg cgtgaccgat cccgccacga
    12241 tggagatcgt ggagatggtc ctcgtgggcc aggtgaacaa ggagatcgtc aacaacatca
    12301 acatggtggg cgggaaggcc gtgggactct cgggcaagga cggcgggctc atcaccgccg
    12361 agcggatcta tcacgatcgc gagggcgcga agatcgacat cggccaggtg gggacggtca
    12421 agcgcatcaa gccctcgatc atcgaactcc tggaccgcga ccgcttcatt ccggtgatcg
    12481 cgcccatcgg cgtggacgag accggcatca cctacaacat caacgccgac accgtcgccg
    12541 gaaagatcgc ggaggcgttg aacgccgaga agctgattct gctcaccgac atcgagggcg
    12601 taaagctcga caatgagctc gcctcctcga taccgaagtc ggacatcccg tccctcatcg
    12661 gctcaaagca gatcggcggc ggcatgatcc ccaaggtgaa ctgctgcctg gacgcgctct
    12721 ccggcggcgt gcacaagacc cacatcatcg acgggcgcgt ggagcatgcg gtccttctgg
    12781 agatattcac cgacgccggt atcgggaccg agatcgtcca ctagtaccac gcggcgcaac
    12841 agtcacaccc tccgcaccac caccagcgca tccaccgcca cgggcagttc gccgcgtacg
    12901 atgagcgggt tgacgtcgat ctccgcgatt tcgtcgtgat cgaggccgag ccgcccgagt
    12961 cccacgaggg cccgcgccag cgcgtcccga tcaaccgcgg gcattcccct gaatgcgccg
    13021 aggagcgatg cgccgcggat ctcgttcatc atctcatgcg cgtcacggag cgaaagcggc
    13081 gcgacccgaa aggacacgtc ccggagcgct tcggtgtaga tgccgccaag gccgaacatc
    13141 acgcagggcc cgaactgcgc gtcgcgcata aagccggcca cgaactcgcg cgtgcccgac
    13201 accatttgct gcaccagcat cccgtcgagc atcccgccgc cgcgctcctc gatggcgcga
    13261 tacgccttta ccagcgcctc gtccccgtcg atgccgagct cgatgagacg gcgctcggtc
    13321 ttgtgcgtga gcgtgctcga cgatcccttg agcgcgaccg gatacccgat tgcgcgcgca
    13381 tgggcgagcg cctcatcgag ggttgcggca tggcgctctt ccgtcaccgg aatcccgtat
    13441 tcgcgcagca cgcgcttgga ctcgtactca ttgagcgccc cgtccccccg cgacacgccc
    13501 gcgccgatga tgccgcggaa ccgatcgaac gatccggcat cgcgtatcgg agcagtatga
    13561 ttctgcgtat cgccatgcat cgcctcacga gcgcgcgcat accgcgacac cgccgcgatg
    13621 gcgcgcaggg cgttcttgac gtcatcgtac accgggattc cggcgtcgat gcagatggcg
    13681 cgcacctcgc gcacggccgc ctccagctcc atcgcctccg cctcctggcg gtactgcggc
    13741 atgaccagca cgacgggctt tcctgtcgat tccgcgacct cgcggcacac cgcggcgagc
    13801 tcccgcccgg gtatgaaatc ccgcaggatc gccgcaccca tcaccttccg ctgggccatg
    13861 aagtggtaga ccaggaatac gagaatgtgc acgtcgatcg cctcgtcctc ggatgcgcgc
    13921 agcaggatct gccggatggc cgacggcgaa acaaatggat tggccacatc gatcgggttc
    13981 gtcgcgctcg atccgggctt cggcagaatg tccatcaccg cctcccggag gtcggcgcgc
    14041 aggggcggga tcgccaggcc gaacgattcg gccgcgtccg ccgccccgat cccgagcgcg
    14101 ccgcccccgc cgacgatcgt gcatccgtga tagggcctga tcgggatcat cgagaaggcc
    14161 agcgaggcgt cggccaactc ctcaacgctt tccagctgta tggcattgca ttgccggagc
    14221 gccgcctccc agaccgctcg ctggccgctc atcgaggccg tgtggctcgc cgccgcacga
    14281 ccgccggaat ccgacaaacc gcccttaatc accagcaccg gtttttccgt tgttgccccg
    14341 cgcagagccg agaagaagtc gcgcccgtct acgacgccct cgatatacat gccaatgacc
    14401 cgcgtctcgc tgtcatcagt gaggtagcgc agcatctcgg tctcccgaag gtcgcagcca
    14461 ttcccaaagc tcaccacctt cgaaaaacgc actccgcgcc attttcccat cagcgcgaaa
    14521 tcgaccgcgt ggccgccgct ctgcgacaga aaggccaccc cgccgggttc acgggatagt
    14581 tccggacccg gtatgagggt ttgcccggat cgcgggcaat agatgccgaa gcagttgggg
    14641 ccgagcaccc gtattcctcg cgccgcgatg gccttcacct cgtcctcgag ggcggccccc
    14701 tccggcgttc cgctctcccg aaatccggat gaaaggatct cgacgccggc cgcccctttt
    14761 tttacgcatg cctccacgat cgccgggaca tgctgggccg gaacggcgat aaccgcgaaa
    14821 tcgatgtcgt ccgggatgtc ttcaattgaa gggtagatcg tcaacccctc ggtggtacca
    14881 ccgcgtcggt tcacgggata aagcgttcct tcgtacccga tcgagaggtg cgacagcagc
    14941 atgcaccgtc caaaactgaa tccctccgcg gtcaccccca cgatcgcgat gcgccgtgga
    15001 tcgaacatac ggtcgattcg cttaaatgag tttctttcat ttacgtgtgc catatctcct
    15061 ccggccggcc cggatacata cgggcgtttg tattgagatt acgaaccccg gctgtttacg
    15121 gcttgacaaa atcatcgtca caaacgataa cataccatac agtatggtat gttttggagg
    15181 ccaaaatttg tcaagcctaa aagacgcaaa aacatcgtgg atcgaggaag gattgaggat
    15241 tctggaaacg aagggaccgg cgatgctatc gatcgacacc ctcgcctttc gaacggggaa
    15301 aaccaagggg tcgttttatc atcacttcaa cggacgggaa gactacatcg cgcgccttct
    15361 cgaacactac gaaaaaaccg ccacgagcga ggtgattcag ctcagcagcg acgccgtcac
    15421 cccgcgcgcg cgcctcgccg cgctcaccac gctcaccttc cagctttcca gccggctgga
    15481 gttggccatc cgcgcctggg cgttgtatga tccgctcgtg cggcggttcc aggaccggat
    15541 ggaccgacgg cggctctcat acctcgagga gctgcatacc gccgggggca tggcccccga
    15601 tgccgcccgg acgcgctcgt accgcgacta tgcgctcttt ctgggcctgc aacagctttc
    15661 gcatcatcat gcggaatcgg agtttcgatc gctcattaaa aggctattca ccgacatctg
    15721 agcgcgtaca gacgccgcat cagcgcctaa tgcattcgat caaaaaaatg attgcgcttt
    15781 tctgtgtcct ctgctgtatt atgtctcaca tagagaacga tcgatccgtt ctccatgaaa
    15841 cataacacga gaggaaaccc aatgaaagca aaactcatcg ccgccgttct cgcgctcgtc
    15901 gtcggaatcg tcttcatggc gcccatcgcg aaggccgaag tcgaaaaatc gaagtgcgcg
    15961 ggactcaaga aggccgaatg caagaccgac aaggactgca cctggatcaa caagcacaag
    16021 gcctcggaca gcttctgcaa gtgcaagccc ggcaagtgca aacccgcgaa atagcgacac
    16081 gcatgattgc aggacccaaa acccgcggaa ttatccgcgg gttttttgtt caattctcgc
    16141 gcataccttc ttgacttttt aatcacgctt gcacatgctc catttattgc acaccgccaa
    16201 tggaggaaac ggcatgacta ttgcccgaac aataaccgca ctctgtctga tcgccctgat
    16261 cggcgcgacc gcttgtaaga aatcccagcc gcaccttgtg ctggtcaaag acaccgtcga
    16321 aaaatcgact gcggtcgtta aaatcaagat agccggcgtg gtgaaaaacg aggaaaaatc
    16381 acgctactgg gtgaacatcg taaaggcggg aagccccgaa tcggagtggg gttcgtggat
    16441 atgggtgagc gacgggacga cggagctcga catcccgatg ccgcgcgatg tcgtcgaggg
    16501 cgcctatgag gtgcgcctcc acagcgacta ccccgtgaaa accttcaaca tcatcgaccg
    16561 caaacccgtt acgataaaat aattcccgat tcaatagtag ggatggggca gtgccccatc
    16621 cctatcgtcg attacaggat aaccccccgc aaccgcaacg ccacgaccgc gatcccctgc
    16681 gcgttcaggg cgatatcgtc ggccaggtac tcctgcatgg actgcgagaa gacgatcccg
    16741 cgccgttcca gctcggtcct gaaaaaacgc tccatcggcc ggtagcagaa ccgctccagt
    16801 tcgtcgtcat cgagtcccga ggcgatggcc tccgcgagca tcacctccat cacgtcgagg
    16861 gagcgcagca tcatgtcacg tacgcgcgcg acgtgcggga ccgccccgta atgcgtggga
    16921 taggccaccg acgcgccggt ccccgcaatc cgctcgatgc tgtcgcgcgc ctccgcgggg
    16981 tcgaaatcgg acgggctggt ggacggaaag acaaacggtt cgtttcccgt ctgcagcacg
    17041 ggataggcca gcccgaaggc gtccccggtg aagaccgcat tgctgccggc atcgtatatg
    17101 cacatatgat gcttggcatg ccccgccgtg tggatgaaac gaagcgtgcg cgctcccagc
    17161 gcgatctcgg cgccgtcgtc catcgatctg acgcgcgtcg cgtcgaccgg ctcgatatcg
    17221 ccgtatcggc gcgcgaaggt ctcgtcgcca tacaccgcgc gcgatcccgc gacgagccgc
    17281 gaagggtcga tgatgtgacg cgccgcgcgc gggtgcgcaa ggacgatcgc attggggcac
    17341 aagcgcgcga gctcggccgt cccgccggca tggtcgagat gcacgtgcgt cacgatgatg
    17401 tattcgacct tttccggttt ccgcccggcg ccggcaagcg cgtccagaag gcgcgggacg
    17461 gcgaagcgcg tattgttgtc gatgaacgcc gcgcgatcgc cctccgtgat cagatacgcc
    17521 gcggcgaact cggggtgcag atacccgcaa tcgatggtga caaccccgcc gctcatcacc
    17581 tgcgcacccc gtgtccgagc ttcccctcga gcgaggcgat tttcccctcg agccggccgt
    17641 ccggccccct ccgccaatcg acggcgagcg tcaggtacac gcgctcggag gagcggccca
    17701 tcgtctccag gcaacgcccg acgagcgcgg cgatctcgcc ccactccccc tcgatgacgg
    17761 tccccatcgg ccccaccgca tgcggaagcc cgctgtgctc gatttctgcg accgcgtcct
    17821 tcacgtatcc gctgaggctt ggccccttgt ccaggggaaa gatcgacatc tgcaccacgg
    17881 cgctcatgga tcgcctccga taatgtttga tgaaaatatt cttgtcaaac ggcctatcgc
    17941 cccatacgca tacgacaaac gagcgacaca acgcacgaga acccggaagg cctgtatgaa
    18001 tgatcgcatc cttcgcgccg tcgttgcaat cataattatc gtttgcgccg catttttatc
    18061 atgcgccaat tccagcgcag ccgataccga cctatacggc ggcgtccgcc agcgcgacta
    18121 cctcatcggt cgctacaatc cgtccgcgga gaagctgttc atttccctgg gaacggtggg
    18181 cataccgacc aggggccgca cgcagtatct tcgcatcgaa gccgcgctgg cgctcaagca
    18241 gctctacaag catcttaaaa ccgaccatcc ggagatatcc ttctgggtgc aaagcgcaac
    18301 gcgcaccttc gacgatcagc gcgccatctg gggaaacaaa tggaacggcg tgacccgtgt
    18361 ggacggcgtg aaactcaacg aaacgatcgc ggaccccgtc gcgcgcggtc tccgaatcct
    18421 cgagcgctcc tccatgcccg gcacctcgcg ccatcactgg gggacggacg tggacctcaa
    18481 cgagcttttc aacggatact tcgatcgcgg aaacggcgcg acactatacc gctggatgac
    18541 cacacacgcg caccggtacg ggttctgcca gccgtacacg gcgggtcgca gcgccggcta
    18601 tcaggaggaa cgctggcact ggtcgtatgc gccgctatcc cgtgcgtttc tcgcggactg
    18661 gatgaagcat tttgggaaaa acaccgacgc ctttaccgcg aaggggctct ttgccggctc
    18721 tgaacacatc ggcggccggg cgccgctgta cgtttcgtcc atcaatcgtg cgtgcgagtg
    18781 attcggacca tgcgccgtca cctctcatgg ccgctcttca tcgcccttgc cgcggcaacc
    18841 tgctcgcacg gcgaggacgc ctcgtattcg aagcgcgccc ccattctcaa tcgactcacc
    18901 gcgacgatcg gagcgcaatc gccggtgcgc tggcaatcgc gcgatcacac gcccgcgcct
    18961 gtgcccgtgg ccgccgttgg cgatccacgc tacacagcgc gcttgtccgg aggcatccgc
    19021 aacatcgata tcgcggccgt gatgaagcag gcgctttccg atggtgtgtc ggttatccta
    19081 gtgatcgggg acggctttgg cgaatcgcac cgtaaaatcc ttgcaacgat gcggccaacc
    19141 ggcaccggac gcgtcggctc ccatttcgaa cgaatcgcga acacgggcgc tcgtggcgtt
    19201 tgcgacaccc gatccgccgg cgggattact accgattccg cggccgcagc gaccgcgctc
    19261 gcctgcggcg tgactaccgc caacggcgtc atcggacgcg ccctaagcgg cgcacgctgc
    19321 gatagcgccc ttgccctcgc cagaaggcgc ggcatggcga ccggcctcat caccgacacc
    19381 cgcgtgaccg atgcgacgcc ggcggcattc tttgcccatg tcgcgaaccg ccgccaggag
    19441 tcggccatcg ccgcacaact tgtcgatggc gacactcccg acatcatctt gggcggcggg
    19501 gcctgctatt tcattccgcg cacaatggcg gtgcggagcc atcccgcact gaacggccgt
    19561 tcggttggcg ccgacttgaa gtcccgtcgc gacgatgacc gcgacctcgt cacagagatg
    19621 gccgcacgcg gatatcgcgt cgtgacgaac acggagatcc tcatttccgc ggcgcgcgga
    19681 tcaaaccgca tactgggact tttcgccggg cacaccatga acacgacgat cgatcgcgac
    19741 gacgagaata ccggcgaacc cggcatcgac ctcatggccc ggaccgcgct ttccgttctt
    19801 gcgcgaaacc cgaagggttt tttcctcatg attgaatgcg ggcagctcga cctggtgtcg
    19861 cacatgaacg acaccggcgc ggtcgtcggc gctctccggg agatggacga cgtcctgcgc
    19921 gtctgcctcg accacgcgcg cgcgaactcg gcgcggaccc ttctcatcgc gaccggcgac
    19981 cacgagaccg gcgcgcccgt catcgacccg cagatcgccg gcatgcgcgc gccggcgctt
    20041 tcgctggacg cgatcgcccg acagcgccga tcgctgcggc gcattgtgat cgatgcgcgc
    20101 tccacggacg agctccggac gcggatcgac acggagaccg cgttcaggat caacctcgaa
    20161 gaggcgaaga tcatttgggg gcagtgagcg acatcagcgg cgaccccggc ctcgcgcttc
    20221 atgaaatcga ttgcgacgcc ctcggcgcct ttctcgaatc gagcgcgcgc gcgttgatgg
    20281 aaatcgaggt gctccgccac gtctttggga tcgatcgaat gcccgccgat cgtcgggggc
    20341 tcttcgcggt tcatttctcg ttgtaccacg cgctctatcg gctcaaattc ggggcgggcg
    20401 cgcggggacg ctacctgcac cttgatccga tgcgcatacg tctcatcgcc gtccccgtca
    20461 cggggtgccg ctggtattcg cccgaagaag ggcgcttctg cgggctttca acgaccaggt
    20521 atgatcgctg cgcgcttcac gcgcacccga cgggcatgcc cgacgaaacg ccgtcattcg
    20581 atccgctgca ggaattttat ctcaatccgg gaaacatcgc cttcggcgag agcgacgtac
    20641 tagagcgcct gatgaacggc gtggtgctct atgccgtacg ccgcggggag atcgagcagg
    20701 cgttgcggct gttcgatctt tcccggccga accgcccgtc catcgaccgg cggtatcatc
    20761 atctcgcccg ggtgcatcac cccgacatgc ccggcggcga cgcggagcgg atgaaggaga
    20821 tcaaccggtc gtatcaggta ctcaaggagg tgttcgtggt gtgagggatg ctactatggc
    20881 tatatcgcag ctggatgtaa tgtgaatacc ctcgcatagt tgcttgcgag cgtaaggtca
    20941 tcgttccgaa gcgcctctcg aacagtatcg attttaacgg catcatccaa tgaaagatag
    21001 gggccccacc cctcctcaga atctaaaagc tcaacatcga tctctgcagc atatacgcct
    21061 tcatgaatat atttaacatg tttccttttt ttcattcttt cctccttgtg aaaccgtccg
    21121 tccatcgttc aggatcaggc cggtaggcgg taaccaaaac cgccgtcgaa gtatcaccct
    21181 tcgacatccc ccagacaaca tggattggat taccttcaga atcactttgt aacaccaaca
    21241 cacatggtcc ttttcgatac tctggatact cctgaataag aacagcgttc tttataccgt
    21301 tgataacgtc gagcacagaa atcgaatcgt ttgatagctc gtcatacccg tgataagaaa
    21361 taaacacctg gcgttgttcc acgagaaaaa cgatgttctt caatattgag ctcatacaat
    21421 gtacacaaga aaaaagccgt agtcaatatt tttagaagat cagcatggga gcatcgtaac
    21481 atttccggta ttatcggaat atattgttat cgatgcaacc gtcatgcata atccgaaaca
    21541 gccgattgcg tcgttcaccc caccgcgttt ttgaactcct cgaaccccat cttctcgatg
    21601 agggcgccga gccgctgcat acgcgcgccg ttggccttgt agaacgcgat gatccggtcg
    21661 atcgccgcga aggcctccgc ctcgctgatg tcgcgaacga gctcctggga aagacgcggc
    21721 ttgcccccgc cgttcccgcc gacgaggagc cgccacccgt ttttcatccc caccaggccg
    21781 atgtccttga tacaggtctc ggcgcactga ttggggcagc cgctcacgcc gatcttgagc
    21841 ttgctgggaa gctccatccc gtggaacgtt tcatccagct tcatcccgac gcccaggctg
    21901 tcctgcatgc cgcgcttgca gaacgtggtg ccgggacagg ccttcacgct gcgcacgcag
    21961 agccccacgc ccgcgcccgg ctccatcccc agatcggccc acaccgcgtc gatgtcctcc
    22021 tcacgcagtc ccacgatcgc gatgcgcgcg gcgctggtca gcttgagcgc ggccgcctgg
    22081 tatttctcag cgacatccgc gagccttcgc agcgtctccg gcgacaccac cccgcagggg
    22141 atgtgcggcg ccaccgcata ggtcgttttg tcccgctgta tgatgactcc cttatctcca
    22201 tcttcaagca tagtgcgcac ctcgattgaa ttgttattgc ccgacgatcc catcatgctg
    22261 cacatcgtgc gtatggcaga tcgaaggcaa agctaccagc ctggcctgcc gatgcaatcg
    22321 tttctatgcg tggtgaacga gatttcgccg agtcagccgc ccacatcgac ccagcgaaag
    22381 gccgggaaac ggacctcacg accgtccaca aagccccggc gctcccgtgc gaacagcagc
    22441 gatcggaccg tggcgctgtg aaaatcgtcg tagcggaccc atatatgggt gtcgagcacg
    22501 tcgttctcat catacgccac cagcggctcg agttcgataa cgatattgga aaacaccagg
    22561 cgatcgggcc gttgattcca gccgaagaga tcgtcgattc cgcgaaaggc cagggcgtcg
    22621 acgatcttct tttcgtagtc gtggaagacg acatacgccc ctatatgcgg gcctgtcggt
    22681 ttcccgccca tgaggcagtg agcgtcgcga tccgtgtata tgctaaacac ctcgctcgac
    22741 ggagcaagga ccaaacgcgc cgattgtgga atcctggacg gttttccccg cagcgacagg
    22801 agcgcatctt cccgcagtgt gaagaaggag ggccgcctgt cggcatggaa gccgatattt
    22861 cccgaatcgg gcattacggc gccgcccatg atgcgggacg tgatgatggc ccaggagttt
    22921 tcgatgtatg cgctgcgaac ggcgccgttt cgatgaaatg aataccggta cttgcccgga
    22981 tggaaccctt gcgccttcct tttctcaaca tttgtagaaa acccgggaaa atatctttcc
    23041 gtgagccggt cgatatatcc cgtcccctca atcatgccgc tctcaatcgc gccggtatcg
    23101 taaaagcgca ccctgccctt catgaatgtg agcgaaacgc cattcatgat cgccgatatc
    23161 ggttcggcga tctccgcctc catgagcgaa ccgttcgcat ggaaggacac gcttccgggt
    23221 gcgcaagaaa tcatgcgccc acccgcccgg tgccgcgaag ataccgagaa cgaaaccatc
    23281 gcgattctac cgttttcgtg caattcggcc ctaccatatc gattcttgaa aatcgtatgg
    23341 ccctgcaccg tgacatcaag gggttctttc accgaggccg actgcaccgc cccgctccgg
    23401 aaaaaactga accagctctg cgcaggggcg gtgaaccgaa cgacctgcat ggagaccgtc
    23461 gctgaggcac cggtaaacac gctcgcaacc ttcccgtcgg catacagctc cacaagcgaa
    23521 ccggcgggga tttccatgga cacgccgctc gcctcgggcg gaacagcctc cgccagcgtc
    23581 ccgcgcatca ccgatccatt cggatacagg gttatggtcg tattcttttt aaaacgaagc
    23641 gatttgttcc ccgcgcccag ggtcgcatcc tcggccagca tggcgatccg gacatcgccc
    23701 cggggataaa aatacgccac atgctccgaa gtattgccac gcgccagcgt ggcggtatac
    23761 gtgccgattc gcacccgggt gtctcgggca aggagacccc ttgagacggc gccgtttccg
    23821 aagaactcga tcgactccat ccatttttct ttcattgtac gggactctaa gacaatatcg
    23881 ttggcgccgc aacgaacgcg caccgttgag tcaagcgtcc ctttttccac ggagccgttg
    23941 tagtaaaact ccatccagct gcccttcgcg aacatgaaat cggtcccgcg gattcgaaag
    24001 cgcgccgacc gggcaagctt gcctcttcgc accatgggac catcgaagcc gggcaggtcc
    24061 cggttgacga catggagccc ttccttgtaa aaacgatact cggctccatg aatcatcacc
    24121 gtctcaacgg gttttattct gattttttcg tacagaacat aaaacggaat gaggcacacg
    24181 gtgtaccatg gcaaatcgat cttgataaac gccgtggcgg gcgctgcgga aaagagcgtt
    24241 acgagcagta caaaggcggc gatactcctc gctggacgat ttcgcatcgc attcaccgtg
    24301 ttatttctct ctaggccgat tcgccttcgc gtacggagaa ttgcatcgta tcatacggtt
    24361 cgcgctccgg cacgtgcatg ctggcgacgg aaccggcaca tgtccttaaa agcgactcct
    24421 ctcgtcgatc ccgtgcgccc gcagcgtcgc ctggattctg gccaccagcg cgtgaacggc
    24481 gctttgcgtt tgcttgtcca ggccctcgaa cttcgtcatg ttctccatca tgaaaaaaat
    24541 gaacgcggcc tcttgttcag aaaactcgat cttcatgctt cccccgcatc aggtgttgat
    24601 atacaccggc atactatttt cttcgatccg gcgactggaa ccttcacgtc ggacgggatc
    24661 ctccacgacg aacttcacat catttcaacg cgttcgcgta tacgcaccca tcagctgtcc
    24721 ctgggcgacg atatgtccct gcagtgcgcg caacagctcc gccttggttt tcccgggctc
    24781 aagattcagc gcgatatcga gcgtgtagat cgtaaaaaaa taccgatgcg tacccccggg
    24841 cgggcaaggc cctccgtatc cgatcttccg gaaatcgttc actccctgct tcgctccgcc
    24901 gggaagcgtc tgtgtcgttg gctggttttc ggcaagagcg gttgtagacg gagggatatc
    24961 gtacatgacc cagtgaaccc agtctccggc gggcgcgtcc ggatcatcgc agataagcgc
    25021 aaggcccttc gcgcctgcgg ggacgccggt ccacgaaagc ggcggcgaga cgtcgggacc
    25081 gtcgcaagtg aaccgtgcgg ggatcgtccc gccgtttcgg aatgccgatg atgtgacgat
    25141 tatcgtttcc atgggatctc ccgcaaaccc atgacgcacc cgtatggcga tcaccccgca
    25201 ggcatcgcct ctgcgcgtca tacggtacga tcattgcata ctcatcattc ggcgtgatgt
    25261 caaacaaaaa gccggtttcc cttccattgc gcagggcgga aaaccggccg aagccgtcac
    25321 tggcgcacga cactcacgga acgttgtcgt acggctattc aggtcgatag atcgccgcgg
    25381 cgatgactcc cccgataaca tattgcgcca tcccgtatcc gaaccagatg agtgtcagcg
    25441 agaagggaac gggatacatc gcatattgcc cgaacatccc catcacattc atgaacaaac
    25501 ccgcgacgat gccgaagcgc accccctcga gcaggccctt cccctcgtat cccttgacga
    25561 agatataggt gaacgccccg gcgagcacga ccccggccag atacatgatc cacatatatg
    25621 acatcatgtc ggagcgccac aagtgtgaaa gcgccttgta cgtgtcggcc atgatgatgc
    25681 cgtgaaaaac gaaatcgatt gcctgaaagg cgacatagac cgcaacgctt gcgagaagat
    25741 atttttttat attcacaata aaactcctga taatgtggtt gacgaatggc taatcatgcg
    25801 tatagtatgt tatgattttg tcaacttcaa tatttttgaa ttgtcgccaa tcagagcgtt
    25861 cccactacga gaggaacata gcgacgatta tatctgatgt atccctcggg tatcgaacgc
    25921 tggtccgcct tcgcggattc ggactagtgg ttgacagaac gccgcttttg cattatgtcg
    25981 cgtgtacggc tcggccatga acatcgatca gctcatcgag tatctccaat cgtatcaatc
    26041 cttctcggag aacgtgcgtc tctggatgcg cgtggacgcc ttcgacggga cctacacgga
    26101 ctatcccgac ggcgtcgaac cgaacctggt cgcgcggtat cgtacgcgcg gcatcgagcg
    26161 actgtacgcc caccagcgcg aggcgttcga ccgcgtgcgc gccggagaca acgtggtcgt
    26221 ggtcacgccg acggcctcgg gaaagaccct gtgctacaac ctgccggtca tcgacgccat
    26281 cgcgaaaaac cccgaggcgc gcgcgctgta cctctttccc acgaaggcgc tctcgcagga
    26341 ccagctcgcc gagctcaacg ccatcgtcga ggacacgggg atcgagctgc gcacgtacac
    26401 cttcgacggc gacacgcccg catcggcccg caaggcggtg aaggcggcgg gcaatatcgt
    26461 gatcaccaac cccgacatgc tgcacgcggg gattctgccg catcacacca tctggatcaa
    26521 gctcttcgag aatctcaagt acatcgtcat cgacgagatc cactcgtaca gcggcgtctt
    26581 cggcagccac ttcgccaacc tcatgcgacg cctgcaccgc atctgcgcct tttacggctc
    26641 gcgcccgcag ttcatctgct gctccgccac gatacgcaac ccgaaggagc acgcggaggc
    26701 cctgatcgga cgcgagtgcg cgctcgtgaa caacaacggc gcgccgcgcg gcgagcggca
    26761 ttacgtgatc tacaatccgc cggtcgtcaa cgagcagctc gggatccgcg cctcggccgt
    26821 caaggaggcg gcgaaggtcg gatccttcgt gctgctcaat aagatcccga cgatcgtctt
    26881 cgcgcggagc cgcatgcgcg tcgagatcgt gtcgacctac ctgcggcaca agtgccccgg
    26941 cgtgagcatc gtcggctacc gggggggcta tctgcccacc gaacggcgcc gcatcgagca
    27001 gggccttcgc gctgggagca tcacgggcgt ggtatccacc aacgcgctcg agctcggtct
    27061 cgacatcggc atgctggacg tggcgatcac cgtgggctat cccggatcga tttcgtccct
    27121 gcaccagcag ttcgggcggg cgggtcggcg cggcaacaag tcgctcgcca tcatggtggc
    27181 gacgagctcg cccctggacc agtacatcgc gaacaatccc gattacatcg tgaagtccaa
    27241 tcccgaatcg gccaccatca acccagacaa cctgctcatc ctcatggacc acctcaagtg
    27301 cgccgccttc gagctgccgt ttcacgccga tgagcgcttc tcggcgcacc tcgggtccac
    27361 gcgcgacatg ctggactacc tcgccgagcg cggcgtcctc aagcgcgccg aggacaccta
    27421 tcactggatg agcgacatct acccggcgaa cgagatctcg ctgcgcacgg ccgcgcagga
    27481 gaactttctc atcataaacg acaagcagga caagaccctg cgcgccatgc ccgacccggc
    27541 gggcgtctcc cgacgcgacc ccgcggcgcg gcgaaacccg aaggtgatcg gcgaggtcga
    27601 ttactactcc gccgcgaccc tcatccacga ggacgcgatc tacatccatc agggacggca
    27661 gtttttcatc gacaccctcg actgggaggg gcgcatggcc ttctgccatg aggtggaatc
    27721 ggactactac accgacgccg agacgaagac cgacatccac gtgctggaga agttcgagga
    27781 gcgctcgctc cccatcgccg tattctcccg gggcgagatc accgtgcgca ccacgccggt
    27841 catgttcaag aagatcaagt tcgacacgca cgaaaacctc ggatggggca agatccacct
    27901 gccggagatc gagatgcaca cggcggccgc ctggctcgac atcgacgagg agcgcctcca
    27961 cgcgatggcg ggacgcgaga tcgccgggcg ggtcctctac ggggcggcct atctcctgcg
    28021 gaacatcgcg cccatctaca cgctctcgga cgtgagcgac gtgcgcgtgc agcaccaggc
    28081 gcgatcgaac tacagcggca agccggcgat cttcatctac gattcgaccc ccggcggcgt
    28141 ggggctttcc gagcgcgtat acgccatact tgacgtcatc gccgccgagg cggtccgggc
    28201 gatcgaggtc tgcgagtgcc gctacggctg ccccggctgc gtgggaccgc tccccgcgaa
    28261 cgacgagcac atcaagcggc tcacagccgc cttcctccgc gagtgctgcg gtgaacatca
    28321 gggacaagct taagctctac gaaacgacgg ccccggcaca gtcgacgccc cagccggcga
    28381 cgatgcccga gtcgctcgcc cgcgccggcg gcgaggtcgt tacgtcgaac ggtgaaacgc
    28441 tgtggcggtt caccgagcgc gttcccctcg acgcgatcgt ccccgcctcc gccgatgacg
    28501 gcacggtctg tccgcttcgt ccctatctgc gctacaaccg gcacccggac gacctttccc
    28561 tcgacacgct cgtcttcctc gacctggaaa cgacctccct ctccatcggc accgggaact
    28621 atccgttcgt gatcggcctc gggttcgtcg acggcggttc attcgttgtg gaacagtatc
    28681 tcctgcacga ctaccgcgcc gagtccttct tactcgcgca catcgtgccc cgccttcgct
    28741 cggcgcgcgc cctggtgacc ttcaacggaa agtcattcga tgtcccgttg ctgaaaaacc
    28801 gctaccgcct gtcgggcgtg ccgggctacc cggtggacac gccggtcatc gacctgctgt
    28861 ttccctgtcg ccgcattttc aagagcatct atgcgagctg cagtttgaaa tcgatggagg
    28921 agaaatttct cgggatcgtg agaaacgacg acatcccggg atggatgatc cccgacgtct
    28981 ttttcgagtt tcagcgcacc ggcgccatcg actgcattcc cgcggtgctc ctgcacaatg
    29041 ccatcgatgt gcgctccatg gcgagcctcc tgttcgcgct caacgacatc taccgaggcg
    29101 ttgccgacaa aaacttcacc gcgctggaga cgcgcgcgtt gatgaacatc gcccacgggt
    29161 tgtatcgggt cgaccgggcg ctgtttatcg atctcgtcga gttcatcgga cgcgaggtgt
    29221 tcggcgaccg gcgcctcttc aagaaatacg gcgcggccct caagcgcacg gggcagcacg
    29281 accgcgcgat cgagctctgg cgcgaggagc gctcgctgta ttcgctcgag gagctcgcga
    29341 aacacttcga gcatcgcgcg cgcgactatg gccatgcgct cgcgcagtgc cgggaggcgg
    29401 ccgtgctcat tgatgcgggc gtcctgcgcg tcggtggcga tcctgcgccg gcgggcatca
    29461 tcacatggta cgccgagcga tttgcgaaac gggaaaaacg attacaccgg aaggcgggcg
    29521 atgccgaagc gacgtaagac ggccgatacc gggcccggcg cggcgacgac ggaggtgtac
    29581 accgagttcg cgcgattcta tgacacggtc ctcaagcatg tcgattacga ccgctggtac
    29641 cgctacatca agtccatcat gctgctgtac gtcgagcacc cgaaccgcct gctcgagctc
    29701 ggatgcggca ccggccgttt cggcgcgaag ttttcgcgcg accggtacgt catcttcggc
    29761 atggaccgct cgctggagat gctgcgcgtt tcgcgcatgc gcgcctaccg gaattttcgc
    29821 gtcctttgcg gcgacatgcg gcaattaccg ctcgccggga ccttcgactt catcttctcc
    29881 gtgcacgaca cgatgaatta cttcctcgag tacgacgaca ttcgccgcgt gctgcgttcc
    29941 gtgcggcgcg cgatggccga cgagggcgtg ttcatgttcg atatcacgac cgaacacaat
    30001 atcaatcgcc acttcgacaa caccgtgatg cgccattcgg tccggggcac ggagatcgaa
    30061 tggcgcaact atttcgaccg cgacacacgg ctcgtatatt cggtcctcga cttccgcgac
    30121 cggcggggcg ttgtccgcac ggagaagcac gtgcagcgca tttacgagac ggacgagatc
    30181 ctgacgctgc tcgaagagga aggcttcatg accctcgatg tcttcagtga ttacagcttt
    30241 gaacccgtca gcactacaac ggtcatgacc aatttcgtca ccaggaagaa atgacatgga
    30301 atacgttttc tacgcgatag gactgctggt tggcgttctg gccttcctct atatggtgtc
    30361 cctctacgag gcggggaaga gcaaggtgcg ggagatcata acgcccgacc gcgagcgggg
    30421 cgcgaacgcg gtccaggtca acccaagcgc ggtaaccgtg aataatgccg ttgggcgaca
    30481 ccccggcgag cggatctgcc cgctctgcgg ctccgtcctc acgaaatacg aggcgctcta
    30541 cgcgaccttc atcgacaccg agggcgaccg acgcctgctc atacacggct gccggtactg
    30601 ccaccgaccc gacgagaaac ccgaggagaa aaagaagtcc gacctgtagc tacggctgct
    30661 ccttcccgga cggcttttcc acgcccggca acacctcttc ggagagattg gggaacccct
    30721 cgaaatcggt gccgaacatt ccccgcagcg gcgggaacac gcagaattcg tggaacttcg
    30781 tcttgtagag ctgcacgtcg tccaccatga ggtcgttgac gacgagcgcg atctgctggc
    30841 gaatgatggg tcccatcggg aacaccatcg acccgccctt cttgaggcac tgaatcagcc
    30901 gcaacggccg aattttacac gcgccaaaaa tgatgatcgc gtcaaagatg ccgaggttcg
    30961 accgggggtc ggtcccgtcg ccaacgaaga tgcgcacgtt gtcgatcttg ttcgccgcca
    31021 gcgtgtcctt ggcgcccagc gcgagcttct cgttgtgctc caccgtcgcc acctcgtcca
    31081 ccaggtgcgc aaggagcgcc gtcgaatacc cgctgcccgt gcccacctcg agcacgcggg
    31141 cgcttcgctt cggcgcaagg tgattgagca tctggagcag ggagagcagc ggatcgctca
    31201 tctcgccgta gccgatgggg atgggctcgt cgggcagata cagcttgttc ttgaagaagg
    31261 gatcgaagaa cttcgcccgg ttgaccgcct cgaaggcgtc gagcacgggc ttcttgatct
    31321 tgtgcttttt cagtccgctg ataaaatcgt tctttgtcgc catggccgcg cgtgatcagt
    31381 attcctcggc gccgtggatg cggtcgaggt tgtcgaattt cgtgtacttt tcccaaaatt
    31441 tgagctcgac gttccggatg gggccgtttc gctgttttgc gatgatgatc tccgcgatac
    31501 ccttcttctc ggtgtccttt ttcacccgct cctcgcggta gatgaacatc accacgtccg
    31561 cgtcctgttc gatggcgccc gactcgcgca ggtccgagag ctgcgggcgc tggtcggtgc
    31621 gggcctccac ggcgcgcgag agctgcgaga gcgcgatcac cgggacgttg agctcgcgcg
    31681 agatctgctt gagcgagcgc gagatctccg cgatctgcaa gtggcgctcg gtgcgcgaca
    31741 ggctggagat gagctgcagg tagtcgacga tgatcacgcc cagctcgccc ttctgcgcgc
    31801 gccggcgcgc cttggcgcgc agctcgagga tgttgaccgc cggcgaatcg tcgataaaga
    31861 taggcgactt cccgaggcgt cccgaggtct gcacgagcgc gttgagctcc tcatggctga
    31921 tatggccggt gcgcacccgc tgcgaatcga tcatggcctc aatgcacagc atccgcgtcg
    31981 cgagttgcgt ggccggcatt tccagcgaaa aaaaaagcac cggcttccgg tcgcgcagcg
    32041 ccacgtggtt ggcgatattg agcgcgaagg ccgttttccc catcgagggc cgcgcggcga
    32101 tgatgatgag ctccgattcg tgaaacccgg tgagcgtctc gtccatctcg gtgaaacccg
    32161 atgcgacccc ggtcacggcg cgcttggtct cgtacatctg cccgatcgcg ttgagcgtct
    32221 cgggaaggat gtcggcgatg ctcctgagat cggtggtgat ccgccgctcc gtcacggtga
    32281 agatgtcgcg ctcgacctcg tcgaggagct cctcggtgtc gcgccccata ttgaagcact
    32341 tctccaccac gtcggtcgac acctcgatga ggcgacggcg cagggagagc tccttcacgc
    32401 ggcgggcgta aaactcggcg ttggccgagg tcgacaccgc ctgatagatc gcgaccagcg
    32461 cctcgtcccc gcccacgcgc tcgaagaggt tcgcgtcgga gaggcgctgc ttgagcatca
    32521 ccagatcgat cggaaggttc ttccgctcaa gttcggttat ggcctcgaat atgtacttat
    32581 gccgctctag ataaaaatcc tccgggtgga ggatttcgag gactttcagg agggccgctt
    32641 tgctcagcag gatggaggcc aggcacgaaa cctcggcctc gatatcctgc ggcggaagct
    32701 tgtcgacggt catcggctag acggcctgtt cgacaatgac cttgacggtc gcgtttagcc
    32761 cttcctcgag cttcagcgtg acctcgtatt cgcccacctg tttgatgggg tcggcgagct
    32821 gtattttgcg cttgtcgatc tcatggccaa tctccttgag cttgcgggcg atgtcgatcg
    32881 aggttaccga gccgaacaac ttcccctctt cgccgacctg cgcgctgatg gtgaccgaaa
    32941 cccccgacag cgactcgatg agcttttcgc tctgcttctt tcgcttgtcc ttcttgatct
    33001 tgatgagctt gttctggtgc tcgatggcgc gctggctgga ctcgttggcg accaaaacga
    33061 gttttttcgg caagagaaaa ttccgggcat acccgggaga cacgtccttg acgtcgccgg
    33121 catcgccgag attggggatg tccttctgca atatgacttt catggcctgc tcctctgctg
    33181 atatcgatgg ataccgcgcc atagttcggc gcgagtgttg tcgtacgtta cggcgcctgc
    33241 gaagggcgca atttcctgaa atcgacccag acatcgagcg atcccagact taccagaata
    33301 acaagaacaa acaatagaaa cggtgcgccg gcgaccgcga caatacccag cgtgagcgga
    33361 agcagaaaca ccggcacgtt cctcttgagc agcaggaact tgatcacccc gagcgcctgc
    33421 agcacataca gcatcgaaac gatgagcgcc ccgttgagcc cgatgcggtg gatcaccaac
    33481 tgctccttcg aatccacgag aaggacgacc agccaggtcg cgatcagtgc gaaaatggcg
    33541 taatcgttca gcgcgaaata ctcaagtccc tgagagagcg cgtcggtatc gccgatcacc
    33601 cgcttgagcg ccatccggat gatgaccacg cccaccgcag aaagcagcag tccatatagg
    33661 aaatggacga acggaatggt ctccacggcg acggccgcat attcgtcgaa acgctcgaag
    33721 aactccttct tgtcggattc ggccatgccc gaagaggcga ccatctcctt gaatacgcgc
    33781 ttcgattcgg cgagcacgtc gatattgcgg tgcagcgtga gcgagtaata ggtcgccgaa
    33841 aacagcgcgg ccagcacaca cgccgacacg atcagaaaga actgcttgga cctgccgtat
    33901 ttcaaagaac acccggcggc cgatccgata accaccggcg cggccacctc ggcgagcttc
    33961 gcgacgtccg ccccgacaat cgcggccagg gcgacggccg cggccatcga cgccgccggc
    34021 ggtatccatc cccaccgggc agacgcatac gcggaaccga ccaggagggc gacgctcagc
    34081 aggatgaagg cgaccagcgc caccttactg gacgataaac ggaaccaggg cgatgatgcg
    34141 cgcgcgtttg atcgcggtgg aaagttcgcg ctggtgtttc gagcaggtgc cggttatgcg
    34201 gcgcggcaaa atcttgccac ggtcggtgat gaaccgttcg agcagctcgt tgttcttgta
    34261 atcgatcttg atgtccttgt tgaagcagaa ccggcatctc ttcttcttga acttctgccc
    34321 gccctgtccc ctcggtccgc cctcgcgata cggccgcctg gcctcgcgtt gctcgggcgc
    34381 cttggtttcg gtggcctcga cgggcgcctc cggctgttgt gctggttcag cgatatgttc
    34441 gtcggacatg cgtaaactcc ttcatgaatg atttcgtgtg attcgcgccc gctaaaaggg
    34501 cacctcattg tcggaaaacg ggtgatcgtc gatgttcgat ccggggccca tgtcggacgg
    34561 ccccgggatg tcgggggtca tggcgtcctt gtcctgtttc gaggtcagga actggaaatt
    34621 ctccacgacg atctcgaccg ccgagcgacg cttcccgtcc tggtcgtccc aggagcgttg
    34681 ctgcaggcgt ccctcgatgg cgatgcggtt gcccttcttg caatactgca cgatgagctc
    34741 gccaagcttg ccccatgcga tgcagctaaa gaaggacgtg tactccttct tgtcggtgcc
    34801 gacggtgtag gagcggttgt ttgccaccga gaagttggtc acggactttc cgttctgcgt
    34861 atagcgcagc tccgggtcct tgacgaaacg gccgatgagg atgatccggt tcagatcgct
    34921 cgccataatg gttcctcgcc gtacctatgc ggttttcttc tgcggcatga ccacgaagag
    34981 atagcgcaga atgttctggt tcagcttgaa ctcggcgatt attttcgcaa cggagtcggc
    35041 cggcgtttgg atgagcaaca gcaggtagtg cgcctcgcgc tcgccattaa tctcataggc
    35101 catgcggcgg gagccccagc tctggtcgtt ctcgatgagt gcgccgtgtt tttgaagaat
    35161 gtctttgacc ttggcgatgt tcgactcaag gtcgctgttg atgcggacaa tgacggttag
    35221 ctcgtacgtt cgcacgatat cccccttcct ttggaaatag tagtctattg ctaacatgcg
    35281 ctgtgctgtc agcagaaagg cccgacaatc ctctggccgg ggctgtttta atcaacaaat
    35341 tttttatttt ttgtattgta ttgtacgcct ttttcgatta tacgtgacgg aatgaaaaag
    35401 atcctatcaa cacacgatct aaaacccgga acggtgctgc gggtgtacaa aaagggcttc
    35461 ggctatgcga tccttcgcgt tatcgaggcc cacgagctgt atcttgcggc gttcgccccc
    35521 gatgatttta tatcgcgggc ggtccccggc gaccgcctca aggcctatct gtggatcgag
    35581 gaaagcggca cctacgaatt tccgcttgtc gtcaacgggt ccatatcccg acagcacgcc
    35641 attctcttct gcggacacac cgaccggatc aagcgtggcg acgccccgaa gtgccttacc
    35701 gcgcggcttt ccgcgcccct gcgttttttt gtgctggatg cgaactcctc gaagggcttt
    35761 acgacaaaac gcatcgtggt tcacacgggc gcgctcgtgg agctcagcga ccgcagcgcc
    35821 cttctccgat acgatggcga tctttccgat ggaacgctca tcaaggggca cctgcgctcg
    35881 ggatcgcaca cggtggacat cctcggcaga gtcacgcgaa gtgcgaccag gggcgcacgc
    35941 cgatatcgca tcgacatatc cgcgatgaat gagcgcgaac ggaacgccgt cctcgattac
    36001 gtcttttccg tctaccgcga atagcgccgt cagcgccgta ccggcgcgcc ctccacgagt
    36061 acgacatcaa acagatacga gtcgcgccgc gagctcagcg gcgtgtagac cttgaacagc
    36121 ccgctgaacg cgaaggcgtc gtttttcttg agcgaggtga gaaacgaccg ctgatcggta
    36181 aagatatagt aggtgatatt catgctcgac gtcgcgcttt cggtcgcgat gatccggacg
    36241 ccccggttgt agcgcgacag ctcgtccacc gaaacgacat gcccggtgcc gttgagaagc
    36301 cggttgacct gctcgtccag aaaaccgtcg cgcatgatgg gcggcaggga ggcgaggcgc
    36361 tcgaagaatt cccggtgctc gatgggcccc gccccgcccg cgggaagtcc ctggcgggcc
    36421 ggcgcggccg ccagcagcag cgacaggacg acgagaatgg cgcgggctcg cggggacatg
    36481 cgcatcagtt tccgtctaac ggtatcgtgt atcgccgcga gccgcggtaa atgccgatct
    36541 tctcaacgcg cccgtcggag cggcgcatga cccccgcaac gtcctccagc gtcgcgacgt
    36601 cggccgcatt gagcgagacg acgatatcgc cgcgcttgag tccgttccgg tatcccagtc
    36661 ccatcggcga gagggcgctg acgaccacgt cgccggtgcg cgcgttttcc gaaagctcga
    36721 tgccgtagcg cgcgtacagg tcgtcggccc cgcgcgacgc gcgcgcatca tccccctttt
    36781 cccgcgacac gagccggatg gtatggcgcc gaccgtcgcg cgtgacggtg acggcgatca
    36841 tggacccggg cggcgcgttc ccgacattcg tcaccaattc cccgagcgag gatatgcgct
    36901 tcccgttgat ctcatcgata cgatcgccgc gcctgatgcc gccgtgctcc gcgggcgagt
    36961 ttttgatgac cgtgatcacc tcgagctgct tgcgcgccgg gcgcttcccg ggcgcgatct
    37021 cgcgtgcgat aaaccccagc cagccgcggc gcacgcggcc gtgctgaatg agatcgttac
    37081 acacctgccg gatgatgttc gacgggatcg cgaagctgat cccctgatac ccgccggaga
    37141 ccgtccgaat ggccgtgttc atgcccacc
//
LOCUS       DAORHF010000006        36213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_609_length_36213_cov_6.529122, whole genome shotgun sequence.
ACCESSION   DAORHF010000006 DAORHF010000000
VERSION     DAORHF010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 36213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36213
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_36213_cov_6.529122"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="PKJ16_01180"
     CDS             complement(<1..954)
                     /locus_tag="PKJ16_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNV45624.1"
                     /translation="MKRTMKGVLVIGCAALILCALLVPAGDLLARAGGAGGGDGGGGG
                     GGDGLDVLIYLLIRVIIELPFPANVIVGGILVAGFIIVSRYSKKKFKAQTILNRLPSG
                     KPVQRVKGFDAFVKNNPDFGQEEFTAKARHAFTAIQEAWQKQSLAGVRRFISDGVYQR
                     FTTQFKMMALLKQKNTIDNLRVINIYIDRIETDGRFDIIHTAIHASITDRFVSELDPS
                     LNSGGAEEFVEYWSFIKKRGKPKTDLYFTETCPNCGSPLPVDMGEVSACPACGTLTNS
                     GEYDWVLSEITQADDYVAANPKLRKSADLTEKVRRLIDENDD"
     gene            complement(997..2475)
                     /locus_tag="PKJ16_01185"
     CDS             complement(997..2475)
                     /locus_tag="PKJ16_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45625.1"
                     /translation="MKRMKAFLLIGIVALAGCAGTGSKRQSTPANSVNVLVAPFEYRG
                     PAEHSWLAAGVSDAVVSGFSRVRAVNVFSDSDRAQAMKEMAFGMTGAVRERDIVTVGG
                     ILGARVIFAGSVQVNGSNVRIVARIIDAESARVERAITLDGRRESLFELQDRVVIGLL
                     DATNQVPSFAQVSPLVRAEETKAVVIRPTTKPDAYAWYAKGLRVHFTRPAEAVGYYTK
                     ACAIDADFFQAYTKMGQAYTETNAFADALRCHERARGILEGGALRESVDYAESLSGTG
                     SVYWGMGESDAARLHHEKALALLAKIGRRDSGMYADILGRVGAAYIQKNELAEGIRTS
                     ERALDMLQAAGLGNSSSAINTLINIGAAYVKKGDKAAAMKRFTRSEEIRERIGFVNTR
                     SHAIAMSNIGSVHFLMGDRDKALASFLRAEEIWEKTGLTNTYDHAMTLSNIGAVYLAK
                     GDRKTGTAYLQRAAVINAKTGRGDLNRAIDAKLKSMNLRRVR"
     gene            complement(2544..3593)
                     /locus_tag="PKJ16_01190"
     CDS             complement(2544..3593)
                     /locus_tag="PKJ16_01190"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV45626.1"
                     /translation="MILFQTRTAAYTAYGIAFGFLFPVISTAVESLFQFGTIGLATIA
                     AVQANSRLLWVIDSAPFFLGLFAALAGIRQDRLHATNARLTREIAARTEVEQRLRKQN
                     ELLHDDLEYARILQRSFLPDLPRCAYADIDYRYNPVKAVGGDLVSIVEIREGGVGFFV
                     GDVAGHGVSASLITALVKVVSDRACRKHGLEPDRFLLLVNRELMQVIPENNYLTALYG
                     VLQRDGGETAFTFSRAAHPHPIVLRGQAGTSELILSDGVPICHGGDSRYECHTARLDP
                     GDRLFLITDGMYDIQQKGKSSIGLAGFLPIIDAANAARRPIGETLDMIVAEADRLHDG
                     QSVRDDRVIIGIEVR"
     gene            complement(3743..4357)
                     /locus_tag="PKJ16_01195"
     CDS             complement(3743..4357)
                     /locus_tag="PKJ16_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNV45627.1"
                     /translation="MPDATILKAIAHCALFVIAVINPVSKIAVLSVLAERNAVRELRR
                     VSLRASVIAAIMLLPFALFGSALLTKVFHVDIHSFQICGGLVLLYRGLLAVNKGFFFE
                     SDPRQSLDDMSVVPLASPLIAGPAAITASVTLPVTFGTPVTLVAIVGALAVNYGVMFF
                     SVSLSAYLDRHHLLGALIRITGLIVSSIGIQMVLDGLTAYLASV"
     gene            complement(4559..6238)
                     /locus_tag="PKJ16_01200"
     CDS             complement(4559..6238)
                     /locus_tag="PKJ16_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV45628.1"
                     /translation="MRKIIGIVSVLLLCAASAWLVSCGGGKHTLGGQIKGDSRLTDEK
                     VRRGRAIEGAAGMHDFASTGMAIRESEERVRDGRLLPLQRVPRDFNTEEYERTAENRF
                     LETLKNPQSTFSIDVDAASYANVRRFINSVGRPPKDAVRIEELINYFTYDYPQPTGAH
                     PFSFTTELSACPWNSSHQLLMVGLQGKTIALEQMPPNNLVFLIDVSGSMNQSNKLPLL
                     KESFKLMVKQLRPVDRVAIVVYAGAAGLVLPSTPGSQKDRIMGVIDSFEAGGSTAGGD
                     GIKLAYTVAKNNFIAHGNNRVILATDGDFNVGVSSTGELERLIEQKRKGGVFLTVLGF
                     GMGNYKDSRMEKLADKGNGNYAYIDTLKEARKVLVSQLGGTLFTIAKDVKLQIEFNPA
                     VVKEYRLIGYENRILAAEDFDNDRKDAGDLGSGHSVTALYELVLADGAALPRSDLLYL
                     TTSVKPGAETADELMQIKFRYKRPKEEQSVLMTHAVLNRPVPAAKTSDNFRFSAAVAE
                     WGLLLKASEHKGRASLDDVQRLASGSIGVDAEGYRAEFLSLVKQSKRLLAR"
     gene            6500..7270
                     /locus_tag="PKJ16_01205"
     CDS             6500..7270
                     /locus_tag="PKJ16_01205"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HNV45629.1"
                     /translation="MDELRNSLMQLFADSGLVALKAGTEVEDMTFAEIAFLKELGDPI
                     LPLIVKIGGPEARNDIRACREIGVHGILAPMIESAYGLRNFVEATADIYRQERRPYLA
                     INIETVTAYAHLDEIMGSPWFDSIDQVTVGRSDLSASMDGAPDDDAVIAATRAIVHKA
                     SALGKNTSVGGKISPANAALVCERIAPSRINTRHMVYDPVRCGDLADAVARGLQFEEE
                     LCERLAEIEPTKRATYRERISATRARIATTPLPCCAAN"
     gene            complement(7313..8590)
                     /locus_tag="PKJ16_01210"
     CDS             complement(7313..8590)
                     /locus_tag="PKJ16_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV45630.1"
                     /translation="MRTILSSIIGHPSRSTLENRIYNALTFFTPLATVFVMPLMFILE
                     WSAYHIASVLVICSVGFYLYFLSRFRKVRSEPVLYVMGFALLVVIWITGYGSIGYVFL
                     FYQFLILYSLLVFKRRVRILLFLLVPAAIIALVSLEYIGITAPRHYHSTDERTVDFVI
                     NFLVWFLFTGFIIKVIIDNYRMERQRIQDELLVAQKLQRRLLPQQVPDIPGFSLHALY
                     LPMELVGGDFYDFCVSDETIDILIADVSGHGMPGAFLASITKMAFEYERSREGPGAVL
                     AGMNAAVRGATVDCNFVTASLCSIDRATKTMRCASAGHVPTLVYRRSSGVVEPVKPRG
                     CPLGLFDDLEVEQAEVRLCPGDRVVLFTDGIVEGFSDQGAMFGMEAFVTAIADGATQT
                     PRAFVDGLIETLRKFSGGDFFEDDITLVVIDVQ"
     gene            complement(8610..9083)
                     /locus_tag="PKJ16_01215"
     CDS             complement(8610..9083)
                     /locus_tag="PKJ16_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45631.1"
                     /translation="MSTHSGVADGFLYAPGVIATLALHSVRFTIAYSVWCTALIVLGI
                     FVSPAAAVPQLIWIANRLYSIIVIWLIAHFVRRSVRDSRQIKQLETMNMITMSAWTKE
                     VNVDGAWISIEEFLKRRFGIEVSHGIDPASSKRILDEIERMKQNGGGINGGPTAI"
     gene            complement(9226..11676)
                     /gene="gyrA"
                     /locus_tag="PKJ16_01220"
     CDS             complement(9226..11676)
                     /gene="gyrA"
                     /locus_tag="PKJ16_01220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV45632.1"
                     /translation="MPKEPGKTIHEKIVPVEIEEQMRSSYIDYAMSVIVGRALPDVRD
                     GLKPVHRRILHAMNERAWRSDRPYVKSAKIVGEVIGNYHPHGDAAVYDTMVRMVQEFS
                     MRVPLVDGQGNFGSVDGDPPAAYRYTEARLQKFAEELLRDIDKETVDFTDNFDETKRE
                     PLVLPASFPNLLVNGSAGIAVGMATNIPPHNLGEVIDATVRLIDNPDMTVKELMKVIK
                     GPDFPTAGIIMGSEGIYNAYAKGKGGIIVRGRIEMEESKKGRETIIITEIPYQVNKAA
                     MISRIAELVNNRVIEGIAELRDESDRTGMRIVIGLKKDANAAVIINQLYKHSPLQTSF
                     GITLLALVNNEPKILNLKGILSNYILHRKSVIVRRTQFDLRKAEERAHILEGLKIALD
                     NIDEVIAIIRASKDADEAGARLIKRFKLTEIQAKAILEMRLQRLTSLEVKKIVEELKQ
                     LKALIADLKAILKSDKRVLQIVKDELGQIKEKYADPRRTEIMHGGEASTSFEMEDLIA
                     EEDVVVSLTNDGFIRRMSVDTFRKQRRGGKGVSGMSTKAEDYIKTMLIASTHDMIFMF
                     SNKGKIFALKTYEIPVASKTSRGKSLKGMINLASGEEISEICAVPEIGEDYYLCMVTR
                     DGIIKKTEVNEFKNAKKGGIIAINLKKEDELVEARLVKGDVDIVLASQHGLLLRTNLT
                     KMRPMGRNASGIIGMRMGKGDRIIGMDVVKPNALLFVVTSKGFGKRMFYKNFPSKGRG
                     GKGMIYLKTSDKTGDASSVRSVFPEDEVIITAKSSKMIRLTANDISIQGRASSGVKLL
                     DIEDDDMVSDFAVITEEK"
     gene            complement(11702..12289)
                     /locus_tag="PKJ16_01225"
     CDS             complement(11702..12289)
                     /locus_tag="PKJ16_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45633.1"
                     /translation="MAGRITFVVGAVLLVLSGGPLAATEPTRKPDLPVAQEIKIPLPP
                     AWNVVEKKKLPNGELRVYKREPLTTDKGKALVPNMVVYVERFATPVDPKAFLISKRLA
                     FPFTRVTRVLTSEDIFGGYRTALGLYGTAYEKRVRTTYTRVLATAVFANSGLTVIINA
                     PERVFPAMEKECFDFLRSIRIIDRPPGAAAPGAVK"
     gene            complement(12297..14195)
                     /gene="gyrB"
                     /locus_tag="PKJ16_01230"
     CDS             complement(12297..14195)
                     /gene="gyrB"
                     /locus_tag="PKJ16_01230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNV45634.1"
                     /translation="MSQNYSADKIQVLEGLDAVRKRPAMYIGSTDVYGLHHLVYEVVD
                     NSIDEALAGVCDTIIVTIHNDNSIEVLDNGRGIPVDTHPVYKISALEIVMTKLHAGGK
                     FDNKSYKVSGGLHGVGVSVVNALSESLTVEVYRDGKTYTQSYKRGAPVGKVKQIGAAK
                     KQGTRVLFKPDKEIFEVLEYSFDTLSKRLRELAFLNAGIKIVLRDDRASGKEHIFQFK
                     GGIVSFVQYINEKKVSISKKPIYFRSQRDTVDVEVAIEYIDSYNETVYTYANNINTKE
                     GGTHLVGFRSALTRVINDSLKKEGYEKKIPQLSGDDVREGLVAIISVKLPNPQFEGQT
                     KMKLGNGEVKGIVESITNEHLSQYFEENPQVVRRILDKCVLSARARIAAKKARDLTRR
                     KGALEGDSLPGKLADCSERDPSLCELYLVEGDSAGGSAKQGRDRNFQAILPLKGKIMN
                     VEKSRLDKILSNEEIKTIITAIGTGIGGDEFDIGKARYGKIIIMTDADVDGSHIRTLL
                     LTFFFRYMPELIRNGYLYIAQPPLYLIKAGKEANYAYNDAERDQVLKKLDKKNTVIQR
                     YKGLGEMNPEQLWETTMNPEKRTILKVNMDDEIEAEEVFTVLMGDAVEPRKKFIEENA
                     RNVRNLDL"
     gene            complement(14192..14428)
                     /locus_tag="PKJ16_01235"
     CDS             complement(14192..14428)
                     /locus_tag="PKJ16_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNV45635.1"
                     /translation="MYIHIGNKRIVSDRRIIGIFNVETMRRSPENAHIVDGTAPEHKA
                     VIVNRNNTTVFSIVSPFTLIKRTALEDTVWSKTE"
     gene            complement(14435..14779)
                     /locus_tag="PKJ16_01240"
     CDS             complement(14435..14779)
                     /locus_tag="PKJ16_01240"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HNV45636.1"
                     /translation="MTRSSRHRHNATVPVSDLMAVAIAGLGMDEAVALERLRIAWPRV
                     VGAIIAAHSRPDRVFKRTLFIAVDHSTFANDVMMMRDSIVKSLDEEMGCSMVKSIKTE
                     VKRIAWGDGAKR"
     gene            complement(14776..15879)
                     /locus_tag="PKJ16_01245"
     CDS             complement(14776..15879)
                     /locus_tag="PKJ16_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HNV45637.1"
                     /translation="MVVERLVLKHFRNYTNLELSPAPGVNIITGHNGSGKTNMIEALS
                     LLSELRSFRGVGDGALMQWDADSYFCRADIVGPDERRFEVGCALYDGRARKRYKIDDA
                     EIRGSAEYYGRFVSVILSPGDLALVDGPPEARRRYVDAVIAKIDRGYLRDLVSFRKVL
                     ASRNRILKDARGEARAPGDQLSVWNALFAELTVAIVARRTEFARTFAPVIQDAYGRIA
                     DDSLAPALSYAPSTGEAGKQDIVERLEGCLRRDLQMGSTTIGPQRDEFVLHDRRGERF
                     ADSASQGQKRTAAIALKIAELDFVERALGKRAVILIDDIFPELDAQRRAAALELFRRE
                     NQTFVTMARDGLGGDGMNGAARFRVEDGRVSAL"
     gene            complement(15900..17024)
                     /gene="dnaN"
                     /locus_tag="PKJ16_01250"
     CDS             complement(15900..17024)
                     /gene="dnaN"
                     /locus_tag="PKJ16_01250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNV45638.1"
                     /translation="MKIEASKDALLKAVSSADSVVSSKAVNAVLMNCLFNVFNDEIEI
                     MATDKEIGIRTRIAASADTEGTFMLSGRRFANIVRDLPEGVIELSVDDRHVVQIRSQS
                     GKVSGQYSIIGTSGENFPEIPRYGDEDVIEIEQAAFRRMIRRVIYAASTDTVKAVFNG
                     VYFVTDEPKTISAVASDSRRLAMNTMSSSGGSDIAQGVILPLKAVREVNSVLGTDGVC
                     RFFIGKNQCFFSVGNTEIVTRTIEGTFPNYSQVIPREYRFRIGIAREPLLESLRRAVN
                     FTVEPVNKIVLRFEGSRLTIEARSPDFGESEENIVIETVPGDPIELGVNALYLIDALK
                     EIDTDVVRVGVTGSKSPMTIAPENDDRYISVIMPLSVKSE"
     gene            complement(17297..18634)
                     /gene="dnaA"
                     /locus_tag="PKJ16_01255"
     CDS             complement(17297..18634)
                     /gene="dnaA"
                     /locus_tag="PKJ16_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016839593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HNV45639.1"
                     /translation="MTHEMWNKVLSGVQTKINKQSYDMWFQNTSVESILDKTVRITVP
                     DDVSRRHIADNYQPLIVELLRELTGEQYFCEFVIANGHTPSPAAQTGIPVPDALSTIN
                     NPSTMMLNPRYTFSNFVVGPNNQFAHAAAVSVSKSPGNTYNPLFLYGGVGLGKTHLMQ
                     AIGHSVMMERPFLKVLYVQCELFINDFINSLRTKTLESFKIKYREVDLLLIDDIQFLE
                     GKEQTQEEFFHTFNTLHQSKKQIVVSSDRPPKDLSTLEDRLKTRFAWGLITDIQQPNL
                     ETREAILRNKAESDSIHVPDEVITFIATRIKSNIRYLESALNRLRHVSETSNEPISIQ
                     HAKTHIKDLFDAEVSKAITINDIMKKVSEKYDVTVDDLRAKGRHSKIVLPRFIAMYLV
                     RKLTSLTTTEIGKEFGDRDHSTVLNAMNNVEKMIKDDPNLKEEIDELIIELKL"
     gene            complement(19009..20646)
                     /locus_tag="PKJ16_01260"
     CDS             complement(19009..20646)
                     /locus_tag="PKJ16_01260"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45640.1"
                     /translation="MQRKNTALIMLLLLLVPAALVAETVIGVSAFTNLAGEKAVQWLE
                     IGIADSVSHKLRNVRDYIVVERTNVDKIIHEVQLGQTGVLDDKKAKQAGKALGADIIV
                     VGNFQKYGNRIRITAKLVEVESHKILKQVESTGVMDNIFELQDEIALKIIDQTNIKIT
                     ADVKNRITENFTKNISAYEYYVKGMSNLLYQLNYLEAIKWFDKAIGVDKNYSLAYSGL
                     GKAYSLRRWELKNYENRIDDSLLEKSYQNSRRALDLSPNLDEAHIALARYYQEVDDKK
                     VPNKWKLCEAETRKAIEINPNNAEAYFLLSRIYGYNDAQEEKYLLISIEKNKFLTDAH
                     NNLAVIYLDQKKLDLAEKYFKNTIEIDPEFKTGYMNLGVVYDRKGQLERALEMYQVVL
                     KKYPTYILGLRNLGIGYRRLNRLDEAMAQFRKAVKVKSDDYQTWSEIAYISLVKKDYR
                     EAIKNYHVALKHNPTYYFTLSNLGYCYGEVGDFKNAVKYLTDCHNQHRDKAWPAGHLG
                     WIYRNKLKDNAQAKYWYGEALKREPNNSGYQQNFNQL"
     gene            20794..21363
                     /locus_tag="PKJ16_01265"
     CDS             20794..21363
                     /locus_tag="PKJ16_01265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV45641.1"
                     /translation="MKILLRGGSIAAGTGVRVGYADILAERLRERGLSLINRSRARDS
                     SFEGNWTFSGDIDPVRPEVLIIHFGIDDIYRPVYRSEFKENLVQLVRLARARFDPAVL
                     LCTSHTWDDEYEMRAADIYYRTVREVALDLACEYVPVHLAWMSRLFEIGRPPKDFLLA
                     DDRLPNEEGHRLYANILWPHLEKFLAPYF"
     gene            21441..21701
                     /locus_tag="PKJ16_01270"
     CDS             21441..21701
                     /locus_tag="PKJ16_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HNV45642.1"
                     /translation="MSVIIKDIRRNDREIIRIEVSEFKGRDLINLRIWYQAVDERGNV
                     VYKPTQKGVALDVSNYAELLDGVERIGNYLKDREAGVNPLGE"
     gene            complement(21682..23154)
                     /locus_tag="PKJ16_01275"
     CDS             complement(21682..23154)
                     /locus_tag="PKJ16_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45643.1"
                     /translation="MTLPGTAASRLIIALLLPDTDDFFRRARPGCTMALSPLRLQSDR
                     AEPGAAASMHLSVTDAPAVHRIEIRNLDLPGELFESLSRHTADACGLSSVLLSMLLKR
                     RMTVAADPAPRGPTAHTGDTDPSVRIDLSVVSTDGDAGCSITLPAPFMRHFIGPGAID
                     PDTLNDAVLAFFDEPMRLLPHLPGLVASLSDSALQSLLYRLMNRRLIGPYQLMVMTMA
                     LPDEAGRIRRNLSPRLVDEVRALMRRYRGLERLTRRDLMAGAYSIEEAVLFLSRGGDD
                     VGFAPFLRAVQAERALLASLRIAVTRDVRDWFLEIAADGLLYAVLSACDEDTVARCIA
                     DDPAWYGGLIGQHVSRRSTESLLSRAGSIAPSPLERLSAHASLVTEYRRQRLRRRGLG
                     AESLPYLLRRIGPGDFRHVLVSAGWFVLSTALKGLRARYATDLVEGLPSGPRFLIEDV
                     LRGVANPDILHDELQIDAARALCVASIARLVEEGIIHPAD"
     gene            complement(23151..24314)
                     /locus_tag="PKJ16_01280"
     CDS             complement(23151..24314)
                     /locus_tag="PKJ16_01280"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV45644.1"
                     /translation="MAMLINDYLQKETPWEILVIDDTRLNRAIVRKTLDRMNIAVTEA
                     ADGQEGLDILARRRFDLILVDIIMPNLDGFGFLERFKDHVRDEFIPVILMTGSDDLNS
                     KIKGLSIGADDFLLKPLNEKELVARVNSLLKLKRAHDELFAKNLQIKKELEIAKKVQQ
                     FIIPKSFAQISYPTISGRYLPIEDIGGDYFDCYTLPDGSVGMLIADVTGHGIPAALVM
                     SMSKMIFSIYAANIGSTSELLAAVNREMKGLLLDNQYITAFYVIYHPAENTIHFTNAG
                     HTRALYFRKSKNAVLLLDTDGLFVGIADDANYGEKALRVEPGDRLFLYTDGISEVRNA
                     ENEEFGEGRIGAFMKARGDLHGDDFCEGLLAELQSFTSLDRRKDDIAFLSIEF"
     gene            complement(24612..28208)
                     /locus_tag="PKJ16_01285"
     CDS             complement(24612..28208)
                     /locus_tag="PKJ16_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45645.1"
                     /translation="MNIHRRARHFLLALATFFLIALVTISPARAATAAPDAPHAFSEL
                     ERAVNAAFSSGDYVALYDAIERLLTAFPDRFESFLYYYDLARLADVHGPARVAATLEK
                     LIAAVERGGGPHRNIARLTLMVELERALYQFDRTRAAAVSARLSPVRTWTLIGPYYRF
                     GDADLRHPFPPELSIKLSDASVHRKIVRVDAASGAVDLSRYLYPDDGVAYAATTIVAP
                     GPVKVRVYAEGPYTLFVNGREAVRNHPSGTSRNLRVLRVEGPRRISLLLKVMKKGDWR
                     FRVLVTDANDAIVPTTARPDETSTDEAACIEEPDVPFPALVERLRSDTAESHFALGTY
                     YDELNSRESIAHYRAAIARRPDPVYRFTLAAALIAYGENAASALASEGWTLYESLNRE
                     HPDFAPARFRRFQRERERRDYRGAFRTGRDLIADAPRYLPARVAHARFLSELGYDTEF
                     DDALRSFRLAFPSSPAPHRLFASHVRAKNPRAAEDALRVALAARNDRSDLTALVRLYK
                     NRGAHQEIVSLLRSRDPEGELIAELIDAYIAKGDEPGARAAIIDALSRREDPYLLLKL
                     GELYYRRGADPSAHWDKMLALKPSLFTMTELMSYLARQRIESPFARNGIGGAEDAILR
                     GVSADTESRPSRILYRGRFFHLMADGGSRVLCEDVYLVKDRKQLERWGEYEIAFAGTL
                     HPVRARVYHADGSFSDSHRIEKINGTNYLNLSSLSIGSVVHIAYVVDFPVTEPRNSSF
                     FSIPLTALSGFDEPVDRCDISVTSPAGQEITLVCNREGVRERTAVAGGVRHAITLTDL
                     PAVRREYDSGPGIGRLPHYAFSSMRDASDFVTWYGGIIANAERIDPAAARRFAAPTLR
                     ETVDRVYEFTAREMDTLRNVLYHPEPAENTLMRMRGTPEDKAVLAKSLLAGLGIRSFI
                     AVARDAHLPPTGAFMSPDAFTHALLYVPLDGRESLWLDFTGRHAPCGAVPEELAGVEA
                     LVLLKNGFEHRTVRDTTPGGIAGRYRITISDAGDAAIDAEVAYEGRTGSMRRDFMNAT
                     YREDRVNAVLGGMFPSMSIDEFRLSNLREYSRPFVIAARGRCDSFATVGHDRIILQPM
                     SQSSRVLDYLHYERRETPLVIRHAISEDDAYEFTLPDRFADAVVEREVRLTARHGHAR
                     IDIKKAKGSRTLRVVKSVRFSRARIEPGEYQDFVKFCVALKTAERHSIIIRR"
     gene            complement(28214..29185)
                     /locus_tag="PKJ16_01290"
     CDS             complement(28214..29185)
                     /locus_tag="PKJ16_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HNV45646.1"
                     /translation="MKGIHFDGTTMRYRTDLPEPVRTAGEALIRVRYAGVCRTDREIA
                     RGYMRFTGIPGHEFIGTVVETDDPSLVGASVTGDINLACHDCPTCAQGRYHHCPTRTT
                     LGIFRKDGVMAEYATLPARNLYVLPEGLSPVHGAFIEPLAAALEIVSVRPFAPELSVL
                     VIGDGILSYLIVGVLSLRGNAVTLIGKHDDKMRIFGERYPRISLRSHRDGVGDDRSRF
                     PVVVEASGTPGGFSLALDHIEPLGTLFAKSTFAGALTVDFNRVVVDEITVIGTRCGPF
                     APAIELLAQGLVDPLPFVEAVISLEEAARVLSDLSGTYKKYVVECTR"
     gene            complement(29182..32862)
                     /locus_tag="PKJ16_01295"
     CDS             complement(29182..32862)
                     /locus_tag="PKJ16_01295"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HNV45647.1"
                     /translation="MPRPPRHIARACIVVLLCAIALAHGAVAHAQPDDESEYFRQKRL
                     GNYDRALEIASDRIMRHDDPIRAEIELVRLFELAETVELVDSAIAALARAESAAPALL
                     ASAVLRARAATLRAQLELRRGSGSAVTSAIDGLGFLTSCDVIGPFHNRGGADFNAMPV
                     NAQRPFDPPASRGRSGEARWFPARTGLTGVFTINNYFKDIDNTFVLLRTVRSVPADGA
                     YQLFVGKTGYCDIWIDGTRVFSNRREHSFFHDQYRISARLTKGAHAILVKIGAADGDG
                     IRVSLRLTDADGRAVPAPVSPHTAIERGSAPAAVSSSLFPSAAGLSNFAAQDPMAAFR
                     LGYLLQATGLAGKSIEEVRALFQRSLAVPVPARWYLAMIEKTAEQREAHLHECLRAHK
                     DHIEALTELAHGKIRTGFLYEAAPLIEAIGRENRASFKYPIVSARLFEALGWHTEGLR
                     LAREAQRSRYPSTGRAIEGIMHLSRGDNAAALPSIEWHYNRERGNPVVIDRLVRCLTA
                     TGDLDRATSVLTHSLVLYPDDPNTLIRLIDIIEQTQGPGAAVPYLGALLQRAPQHAGA
                     LMRLGLVYHRLGNDALARHYLARALRHDQGNVALKRYLTIIENRPDPLDGHAVRDDIR
                     DLARAAARHRDEAAVILIDETMIRVLPDGSYEHRVRKAVLINDRSALDEFTRQYIVYS
                     PETDSIEDIDCGVINDGTRTAVTAFYDRSLSDPESRLFYDVKAKVIPLPSLGPGSIID
                     LRYTRISRVGEIYKGYFGERIPVGGEHRTLVFNCVLAHPRDKSVYPHLRGIDGSPIAT
                     TVGPADTVHRLHLRDRAPYKKESLMPHRSQLVPMLYFTTFRNWTEAHGWYRSLVKDRM
                     TMSAEMRDALRTAVAGASGRDDTIRRIYNHVNAHIRYVGFELGIGGIRPRHADQTYHT
                     RMGDCKDISLVLAAFLRAAGIDANLALVRTRDNGPANLAVPYLGDFNHAICHVDANGP
                     LFLDGTAKLAGMSELPSTDRAITAFVVGDRRYRFIDTAHRRYAENRDDVTNDVYLTPA
                     GDARIVRTLSKRGSYAVGSRSELQDREKKRASISAYWNERFPGATISPPEYGAAEPDE
                     PMVYRYTITVPSFLETDGAEMVFGPFFSASNYYSTYAMLRSRTHPVVFTTTFSSDTVI
                     RYHLPQGFSPVSVPKSEQFVTDDCEARFRFVAREGVIEVVSHIAFKAHRIPIGSYARF
                     RDFTRFVNRKENEKIIIRRNGP"
     gene            32971..33930
                     /locus_tag="PKJ16_01300"
     CDS             32971..33930
                     /locus_tag="PKJ16_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45648.1"
                     /translation="MITPRTVRIVLLVALILALLAVIGRTVFDGDRESRGALGNPFYD
                     IFYRYYSGREDVAKRDLRALFGSTRHGAHALINDGVLRAREGDADGAARSFGHAFDRG
                     ASLALSYLSSLRARTGAGYREFLAENPLVRETQWAEYELAVAAASERRDPDARSHLAR
                     AIARGFASTELMDQEPAFVRMRNDDAYKRLREAADANRASRISLSRLMRAERARTGGG
                     KASYVPEAIETANALERVGRRAEAEQALLPLTASSAPFRDRAMATYRLARIRATMGDR
                     AGSHAYLERFRSMIASGESDPTGFKDCAARFQDDIIRNDPLLR"
     gene            34026..35504
                     /locus_tag="PKJ16_01305"
     CDS             34026..35504
                     /locus_tag="PKJ16_01305"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNV45649.1"
                     /translation="MKTAQTAFMLLMITVLAGAVSCRLKVPLKEMSLAKSAITRAGEV
                     KADKYAPDDLKRAKEFLSLSHADIKKEDTSKAEENAKKSLAYGTDAINKSLPLLSKDT
                     LDEAKKLIADAERMNAERYASSDFSDARSKAGEAEKLHGDKSFWESYLVSKKAIAAAT
                     AARDKAEARIPELQNRIASIRGEADNLKSARGSEFASSELAQVSSRLDAATAGIGQKN
                     LKDASRAIDDAESALNRAKGNTKRGIAAEKIRNAEKELDIANSSKMKDQFAADIQRAS
                     TLITNAKSDLSSGSFDAAATKADEATALLKSANSNMTVKAQELKKTAQEKLEAAENAL
                     KKIKESPLASHMQADIDAVQPLIDQSRGFLNNDAYQDSIAKSDEALARINQINIAMEK
                     KAEELRLKGDTSKEAKEEQVYVVKYNPKKRDCLWRIAYYVYKDAKLWPVIYTANKDKI
                     KDPDLIFPGQKFSIPSMDKKSDDAGKKEKGKPPVEGTVDKRD"
     gene            35523..>36213
                     /locus_tag="PKJ16_01310"
     CDS             35523..>36213
                     /locus_tag="PKJ16_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45650.1"
                     /translation="MNRSALFWGALVLMAAVPWRAVARDINLDEIYLRKSSPALKKLI
                     LYKLDAYERVNAHFVDRDAIFSAWTSGDQIVYIKELPGGSNVVYRYNPHRRRLSEAGR
                     FSGVVTYARLSGRGRYVYLKRFVSDRGIIPRPGMVIIDIAAGRVIERKSNFPFLDFSV
                     AAGSDALYYETKRGIVEYHPGGGEGRMMIPRAEYGDIAVSDNPSVGYLSPNLQRALVV
                     KGGGGSYRAKLV"
BASE COUNT         6654 a        11193 c        11537 g         6829 t
ORIGIN      
        1 gtcgtcgttt tcgtcgatga ggcgccggac cttctcggtg agatcggcgg actttcggag
       61 cttcgggttc gcggccacat agtcgtccgc ctgcgtgatc tcggagagca cccaatcgta
      121 ttcgcctgag ttggtgagcg tcccgcaggc cgggcaggcg ctcacctccc ccatgtcgac
      181 cggcaacggc gagccgcagt tggggcaggt ctcggtgaag tagaggtcgg ttttcggctt
      241 gccgcgcttc ttgatgaacg accagtactc gacgaattcc tccgcgcccc ctgagttgag
      301 cgacgggtca agttcgctca cgaaacggtc cgtgatgctc gcatgaatcg ccgtatggat
      361 gatgtcgaag cggccgtccg tttcgatccg gtcgatgtag atattgatca cccgaaggtt
      421 gtcgatggtg ttcttttgct tgagaagcgc catcatcttg aactgcgtcg tgaagcgctg
      481 atacaccccg tccgagatga accgccgcac gccggcgagc gactgcttct gccatgcctc
      541 ctggattgcg gtgaacgcgt ggcgcgcctt cgccgtgaat tcctcctggc cgaagtcggg
      601 attgtttttc acgaaggcgt cgaacccctt cacccgctgc accggctttc cgctggggag
      661 acggttgagg atggtttggg ccttgaattt ctttttcgag taccgcgaga cgatgatgaa
      721 ccctgcaaca aggatccctc ccacgatgac gttggccgga aagggcagct cgatgatcac
      781 ccggatgagc aggtaaatga ggacgtcgag tccatcgccc ccgccgccgc ccccgccatc
      841 accgccgccc gcgccgccgg cgcgcgcgag aagatcgccc gccgggacca ggagcgcgca
      901 gaggatcaac gccgcgcagc caataactag cactcccttc atcgtccgtt tcatgccatc
      961 accctccccg tgttgatcgc cgcggcgccg atccgcttac cgcacccgcc gcaggttcat
     1021 cgacttcagc ttcgcatcaa tggcccggtt gaggtcgccg cggcccgtct tcgcattgat
     1081 tacggcggcc cgctgcagat aggccgtccc ggtcttccgg tcgcccttcg ccaggtacac
     1141 cgcgccgata ttgctcaacg tcatggcgtg atcgtaggtg ttcgtaagcc cggttttctc
     1201 ccagatctct tcggcccgca ggaacgaagc cagcgccttg tcgcgatcgc ccatcaggaa
     1261 atgcaccgaa ccgatattgc tcatggcgat cgcgtggctc cgcgtgttca caaacccgat
     1321 ccgctcgcgt atctcctcgg accgggtaaa gcgcttcatc gcggccgcct tgtcgccctt
     1381 cttcacgtac gccgccccta tgttgataag agtgttgatg gcgctgctgg agttcccgag
     1441 gccggccgcc tgcagcatat cgagggcccg ctcgctggtc cggattccct cggccagctc
     1501 gttcttctgg atatacgccg ccccgacccg accgaggata tccgcgtaca ttcccgaatc
     1561 gcggcgcccg atcttcgcca gcagggcgag cgccttttca tgatgaagcc gcgcggcgtc
     1621 ggattcgccc atgccccagt agacggagcc ggtgccggac agggactcgg cgtagtcgac
     1681 gctctcgcgc agtgcgccgc cctcgaggat cccgcgcgcc cgctcatgac agcgcagcgc
     1741 atcggcgaag gcgttcgtct ccgtataggc ctgtcccatc ttggtgtagg cctggaaaaa
     1801 atccgcatcg atcgcgcagg ccttcgtata atatccgacc gcctccgcgg gccgcgtgaa
     1861 gtggacgcgc aagcccttcg cgtaccaggc ataggcgtcg ggcttggtcg tcggtcggat
     1921 cacgacggcc ttcgtctcct ccgcgcgcac cagcggcgac acctgcgcga acgaggggac
     1981 ctggttggtc gcatccagca ggccgatcac cacccggtcc tgcagctcga agagcgactc
     2041 gcgccgcccg tcgagggtga tggcgcgctc gacccgcgcg ctttccgcat cgatgatccg
     2101 tgcgacgatc ctgacgttcg atccgttcac ctggacgctg ccggcgaaga tcacgcgcgc
     2161 gcccagaatg ccgcccaccg tgacgatgtc gcgctccctg acggcgccgg tcatcccgaa
     2221 cgccatctcc ttcatcgcct gcgcgcggtc cgaatcggaa aacacgttca ccgcgcgcac
     2281 ccgcgaaaaa ccgctcacaa cggcgtcgga caccccggca gcgagccagg aatgctccgc
     2341 gggcccccga tactcgaacg gcgcgacgag cacgttgacg gagttggcgg gcgtgctctg
     2401 ccgtttcgac ccggtcccgg cgcacccggc aagcgccaca atgccgataa gtaggaacgc
     2461 cttcatccgt ttcatagtct catccatcct tcgttaatat gggataacct ggtttgtcgg
     2521 gataccgcag caccatcgcc cgctcagcgc acctcgatgc caatgatgac ccggtcgtcc
     2581 cgaacggact gtccgtcatg caggcggtcc gcctcggcga caatcatgtc cagcgtctcg
     2641 ccgatcggcc gccgcgccgc attcgccgcg tcaatgatcg gcagaaagcc ggcaagacca
     2701 atggagctct tccccttttg ttgtatgtcg tacatcccgt ccgtgatcag aaacaaccgg
     2761 tcgcccggat caagccgcgc cgtatggcat tcataccggc tgtcgccccc gtggcaaatg
     2821 gggacgccgt cggagaggat caactccgat gtcccggcct gcccgcgcag tacgatgggg
     2881 tgcggatgcg ccgcgcgcga gaaggtgaag gccgtttccc cgccgtcccg ctggagcacg
     2941 ccatagagcg cggtgagata gttgttctca gggataacct gcatgagctc ccggttcacg
     3001 agcagcagaa accggtcggg ctccaggccg tgtttccggc aggcgcggtc cgataccacc
     3061 ttcacgaggg cggtgatgag cgaggccgat acgccgtgac cggcgacgtc gccgacaaag
     3121 aagccgacgc ccccctcccg aatctcgaca atggacacga gatcgccccc gaccgccttc
     3181 accggattat accggtagtc gatgtcggca tatgcgcacc ggggcaggtc cggaagaaac
     3241 gaccgctgca ggatgcgcgc gtattcgaga tcgtcgtgta agagctcgtt ttgctttctc
     3301 agccgctgtt cgacctccgt acgcgccgcg atctcgcggg tgaggcgcgc atttgtcgca
     3361 tggagccggt cctgccgaat gccggcgagc gcggcgaaca gtcccaggaa gaagggggcc
     3421 gagtcgatga cccagaggag gcggctgttg gcctgcaccg ccgcgatcgt cgcgaggccg
     3481 atcgtgccga attgaaataa cgattccacg gcagtggaaa tcaccggaaa taaaaacccg
     3541 aaggcgatcc cataggccgt atacgccgct gtccgtgtct gaaacagtat catgtcccgt
     3601 tacgtgctca ataacttccg cgcgccgccc ggcgccgcat gcggccccgc atgatcaaaa
     3661 catgaaaaaa aaccggggtc aagacattaa ttttacatcg tgggtcgatc cgctgcgcgc
     3721 gccgtcggtc gagtcccttc gcctataccg acgcgaggta cgccgtgagg ccgtccagga
     3781 ccatctggat gccgatgctg gagacgatga ggccggtgat gcggatgagc gcgcccagga
     3841 gatggtgtcg gtcgagatag gcgctcaagg acacggaaaa gaacatgacg ccgtaattga
     3901 ccgcgagggc gcccacgatg gcgacgaggg tcacgggcgt cccgaaggtg acggggagcg
     3961 tcaccgacgc ggtgatggcg gcgggaccgg cgatgagcgg cgaggcgagg gggaccaccg
     4021 acatgtcgtc cagactctgg cgcgggtcgc tctcgaaaaa gaagcccttg ttcacggcga
     4081 gcaggccgcg atagagcagc accagcccgc cgcagatctg gaaggaatgg atgtccacgt
     4141 ggaagacctt cgtgagaagg gccgacccga agagggcgaa gggcagcaac atgattgcgg
     4201 cgatgaccga ggcgcgcagc gacacgcggc gcagttcccg caccgcattg cgctcggcga
     4261 gcacgctgag caccgcgatc ttgctcaccg gattgatcac ggcgatcacg aagagcgcgc
     4321 agtgggcgat ggctttcagg atcgtagcgt caggcatggc gattcactcc gacaacggca
     4381 ctgaggatca tacacgtcca atccgctgtc acgcactttt tcacggtatg atccggatcg
     4441 cgacgcgatc cgcgcgaccg tcgggccgaa gacgccttac acgcactgga tatgggatgg
     4501 tctccgctgc cggcggcggt cccctgcaat cggccgccgc cggtatcgtt gcatcacgct
     4561 atcgcgccaa gaggcgcttc gattgcttca cgagcgagag aaactccgcc cggtagccct
     4621 cggcatcgac gccgatgctt ccgctggcca aacgctggac gtcatcgagg ctcgcgcgtc
     4681 ccttatgctc cgatgccttg agcaacagcc cccattcggc caccgcggcc gagaagcgga
     4741 agttgtcaga ggtcttcgcg gcggggaccg gacggttcag gaccgcatgg gtcatcagca
     4801 cgctctgctc ctccttcggg cgcttgtagc ggaacttgat ctgcatgagc tcgtccgcgg
     4861 tttccgcgcc gggcttcacg ctcgtggtga gatagagtag atcgctccgg gggagcgccg
     4921 cgccgtcggc caggaccagc tcgtagagcg ccgtcacgct gtgtccggac ccgaggtcgc
     4981 ccgcgtcctt ccggtcgtta tcgaagtcct ccgcggccag gatgcggttc tcgtagccga
     5041 tgaggcggta ctccttgacc accgcggggt tgaattcgat ctggagcttc acgtccttcg
     5101 cgatggtgaa gagcgtcccg ccgagctgcg acaccagcac cttccgcgcc tccttgagcg
     5161 tgtcgatgta cgcgtagttg ccgttcccct tgtcggcgag cttctccatg cgcgagtcct
     5221 tgtagttccc catgccgaag ccgaggaccg tcaggaacac gccgcccttt cgcttctgtt
     5281 cgatgaggcg ctcgagctcg ccggtggacg atacgccgac gttgaagtcg ccgtcggtcg
     5341 cgaggatcac ccggttgttc ccgtgcgcga taaaattatt cttcgccacc gtataggcga
     5401 gcttaatgcc gtccccgccc gcggtagagc cgccggcctc gaacgaatcg atcacgccca
     5461 tgattcggtc cttctggctc cccggcgtcg agggaagcac caggccggcc gcgcccgcat
     5521 acaccacgat ggccacgcga tcgaccggcc gcagctgctt gaccatgagc ttgaaggact
     5581 ccttcaacag cggcagctta ttggactggt tcatcgatcc cgagacgtcg atcaggaaca
     5641 ccaggttgtt gggcggcatc tgttcgagcg cgatagtttt gccctgcagt ccgaccatga
     5701 gaagctgatg cgatgagttc caagggcacg ccgagagctc ggtggtgaag ctgaacgggt
     5761 gggcgcccgt gggctgcgga tagtcgtagg tgaagtagtt gatcagctcc tcgatgcgca
     5821 ccgcgtcctt cggcgggcgc ccgaccgagt tgatgaaccg ccgcacgttc gcatacgacg
     5881 ccgcatcgac atcgatggaa aaggtcgatt gcggattctt gagcgtctcg aggaaccggt
     5941 tttccgccgt ccgctcgtac tcctccgtgt tgaaatcgcg cggcacccgc tggagcggca
     6001 gcagacgccc gtcgcggacg cgctcctcgc tctcccggat cgccatgccg gtggacgcga
     6061 aatcgtgcat ccccgccgca ccctcgatcg ctcgcccccg ccgcaccttc tcatcggtca
     6121 gccgcgagtc ccccttgatt tgtccgccga gcgtgtgttt cccgcctccg caggacacga
     6181 gccaggcgct ggccgcgcac agcaagagca cgcttacaat gccaatgatt ttacgcatat
     6241 ctccccctca gttgcgttga ttccggtctg aacgaccgtt cttattgacg cccgtcccgc
     6301 cgacgccgga aaaaaattgt gcggcgtgac gctgcgtctc gatcgagtga ccggcggggc
     6361 cccggtttcg ttccctgaat gtgacaattt ttccccattt acagaccgtg atcgggaaaa
     6421 accggtattg cggtatcgaa cgcgcgccga tactaagaac aggccgtacg cttcgatttt
     6481 tttcggcagg aggacgcaca tggacgaact acgaaactca ctcatgcagt tattcgctga
     6541 ttccgggctg gtggccctca aggcggggac cgaggtcgag gacatgacct tcgccgagat
     6601 cgcctttctc aaggagctgg gtgatccgat cctgccgctc atcgtgaaga tcggcggtcc
     6661 cgaggcgaga aacgacatcc gcgcctgccg cgagatcggc gtgcacggga tcctcgcgcc
     6721 catgatcgaa tcggcctacg ggctccggaa tttcgtcgag gcgacggcgg acatctatcg
     6781 ccaggagcgc cgtccgtacc tcgccatcaa catcgagacg gtgacggcct atgcccacct
     6841 tgacgagatc atgggaagcc cctggttcga ctcgatcgac caagtgaccg tcggccggag
     6901 cgatctctcc gcgtccatgg acggcgcgcc ggacgacgat gcggtcatcg cagcgacgcg
     6961 cgccatcgta cacaaggcgt cggcgctcgg caagaacacc tcggtcgggg gtaagatttc
     7021 gccggcgaac gccgcgctag tgtgcgagcg aatcgcgccg tcccgcatca atacgcgcca
     7081 tatggtgtat gatcccgtcc ggtgcggcga tctcgccgat gcggtcgcgc gggggctgca
     7141 attcgaggaa gagctctgcg agcgccttgc ggagatcgag ccgacaaagc gcgcgacgta
     7201 ccgcgagcgg atcagcgcga cccgcgcccg cattgcgacg acgccgctcc cctgctgcgc
     7261 cgcgaactga tcgcgccgga cagccgcgtt ccgggcgcga ccggcggcgc cgtcactgca
     7321 cgtcaattac gacgagggtg atatcgtcct cgaagaaatc gccgccggaa aacttgcgga
     7381 gggtttcgat aagcccatcg acgaatgcgc gcggggtctg cgtcgccccg tcagcgatcg
     7441 ccgttacgaa cgcctccatc ccgaacatgg cgccctgatc gctaaagccc tcaacaatcc
     7501 cgtcggtaaa aaggaccacc cggtccccgg gacacagccg aacctccgcc tgttcgacct
     7561 ctaggtcatc gaagagcccc agcgggcagc cgcgcggctt taccggctca accacgccgc
     7621 tcgagcgccg atacacgaga gtgggaacgt gcccggcgga cgcgcatcgc atcgtcttcg
     7681 tcgcgcgatc gatcgagcac aggctcgcgg tcacgaagtt gcagtccacg gtcgctcccc
     7741 gcaccgcggc gttcatgccc gcaagcaccg cgccggggcc ctctctcgac cgctcgtact
     7801 cgaaggccat cttggtgatg cttgccagaa aggccccggg catgccgtgc ccggacacgt
     7861 cggcgatgag aatgtcgatc gtctcgtcgg acacgcaaaa atcgtaaaaa tcgcccccca
     7921 ccagctccat gggcagatac agcgcatgca gcgagaagcc gggaatgtcg ggaacctgct
     7981 ggggcagcag acgccgctgg agcttctgcg ccaccagaag ctcgtcctgg atgcgctgcc
     8041 gctccatgcg ataattgtcg atgatcacct taatgataaa accggtgaac aggaaccaga
     8101 ctagaaagtt gatgacgaaa tcgacggtcc gctcgtccgt cgaatgatag tgcctgggcg
     8161 ccgtaatccc gatatactcg agggaaacca gcgcaatgat ggccgccggc accaagagaa
     8221 acaggagaat acggacccgc cgcttaaaca cgaggagcga gtagaggatc aggaactggt
     8281 aaaaaagaaa tacgtacccg atggacccgt agccggttat ccagatcacc accagcagcg
     8341 caaaccccat gacatatagg actggttcgg accggacctt gcgaaatcga gacaggaagt
     8401 acagatagaa gcccacggag cagatgacca ataccgacgc gatgtgatag gcactccact
     8461 cgaggatgaa catgaggggc atcacgaaca cggtcgccag gggcgtgaag aacgtcaggg
     8521 cattgtagat gcggttctcg agcgtactgc gggacggatg gccgatgatg ctggaaagga
     8581 tggttctcat atgtccacga ttcctatcgt catatggccg tcggacctcc gtttattccg
     8641 ccgccattct gtttcatccg ctcgatctcg tcgaggatcc gcttcgacga cgcgggatcg
     8701 attccatggc tcacctcgat cccgaaccgc cgcttgagaa actcctcgat gctgatccac
     8761 gcgccgtcta cgttgacctc cttggtccat gcgctcatgg taatcatgtt catcgtttcg
     8821 agctgcttga tctgccgcga gtcgcgaacg gaccgtcgaa cgaagtgggc gatcagccag
     8881 atgacaatga tggaatacag ccgattcgca atccaaatca actgcggaac cgctgcggcg
     8941 ggagaaacga agatgcccag gacgatgagc gccgtacacc atacgctgta ggcgatcgtg
     9001 aatcggactg agtgaagggc gagcgtggcg atgaccccgg gggcatacag aaatccgtcg
     9061 gcgacgccac tatgcgtgct catatcgacg atcagggcaa cgctcgacca gagcaacagg
     9121 ccggcaatcg ttagtgggat gcgtaactgc attatcgtat gtgtctgttc cgtgcgagta
     9181 tcgcacacgt ggttgcggat atcaagacaa aaacggccga cgcgctcact tctcctcggt
     9241 gatcaccgcg aagtcggata ccatgtcgtc gtcctcgata tcgagcagct tcacgccgga
     9301 agacgcgcgc ccctggatcg agatgtcgtt cgccgtcagg cggatcatct tgctgctctt
     9361 ggcggtgatg atcacctcgt cctcggggaa caccgagcgc accgacgagg cgtcgccggt
     9421 cttgtcgctg gtcttgaggt agatcatccc cttcccgccg cggcccttgg acgggaagtt
     9481 cttgtagaac atccgcttac cgaagccctt ggaggtgacc acgaacagga gcgcattggg
     9541 cttcaccacg tccatgccga tgatgcggtc gcccttgccc atgcgcatgc cgatgatgcc
     9601 ggaggcgttg cggcccatgg gacgcatctt ggtcaggttg gtgcgcagca gcagcccgtg
     9661 ctgggaggcg agcacgatgt ccacgtcgcc ctttaccagg cgcgcctcca ccagctcgtc
     9721 ctcttttttg aggttgatgg cgatgatccc gcccttcttg gcgttcttga actcgttcac
     9781 ctcggtcttc ttgatgatgc cgtcgcgggt gaccatgcac aggtagtagt cctcgccgat
     9841 ctcgggcacg gcgcagattt cgctgatctc ctcgccggag gcgaggttga tcatgccctt
     9901 gagcgacttg ccgcggctgg tcttcgaggc caccggtatc tcgtaggtct tgagcgcgaa
     9961 gatcttcccc ttgttcgaga acatgaatat catgtcgtgg gtggaggcga tgagcatcgt
    10021 cttgatatag tcctcggcct tggtgctcat gccgctcacg cccttcccgc cgcggcgctg
    10081 cttgcggaag gtgtccaccg acatgcgccg gatgaagccg tcgttggtga gcgagaccac
    10141 cacgtcctcc tcggcgatca ggtcctccat ctcgaagctg gtcgaggcct cgccgccgtg
    10201 catgatctcg gttcggcgcg gatcggcgta tttctccttg atctgcccga gctcgtcctt
    10261 cacgatctgg agcacgcgct tgtcgctctt gaggatcgcc ttgagatcgg cgatgagcgc
    10321 cttcagctgc ttgagctcct cgacgatctt cttcacctcg aggctggtga gccgctgcag
    10381 gcgcatctcc aggatcgcct tcgcctggat ctccgtgagt ttgaagcgct tgatgaggcg
    10441 cgcgcccgcc tcgtcggcgt ccttcgacgc gcggatgatg gcgatcacct cgtcgatgtt
    10501 gtccagcgcg atcttgaggc cctccaggat atgggcgcgc tcctcggcct tgcgcaggtc
    10561 gaactgggtc cgccggacga tcacggactt gcggtgcagg atgtagttcg agaggatccc
    10621 cttgaggttg aggatcttcg gctcgttgtt caccagcgcg agcagcgtga tcccgaacga
    10681 ggtctgcagc ggcgagtgct tgtagagctg gttgatgatg accgccgcgt tcgcgtcctt
    10741 cttgaggccg atcacgatgc gcatccccgt gcggtccgat tcgtcccgaa gctccgcgat
    10801 gccctcgatc acgcggttgt tcaccagctc ggcgatccgc gagatcatcg cggccttgtt
    10861 cacctggtag gggatctccg tgatgatgat ggtctcgcgc cccttcttgc tctcctccat
    10921 ctcgatgcgg ccgcgcacga tgatgccgcc cttgcccttc gcgtaggcgt tgtagatccc
    10981 ctccgatccc atgatgatgc ccgcggtcgg gaagtcgggc cccttgatga ccttcatgag
    11041 ctccttcacg gtcatgtcgg ggttgtcgat gaggcgcacg gtcgcatcga tcacctcgcc
    11101 caggttgtgc ggcgggatgt tggtggccat gcccaccgcg atccccgccg agccgttcac
    11161 caggaggttc gggaaggacg ccgggaggac cagcggctcc cgcttggtct cgtcgaagtt
    11221 gtcggtaaag tcgaccgttt ccttatcgat atcgcgcagc agttcctccg cgaacttctg
    11281 gaggcgcgcc tccgtgtacc ggtaagccgc gggcgggtcg ccgtccaccg acccgaagtt
    11341 gccctgcccg tcgaccaggg gaacgcgcat cgagaactcc tggaccatgc gcaccatggt
    11401 gtcgtacacc gcggcgtcgc catgcgggtg gtaattaccg atcacttcac cgacgatctt
    11461 ggccgatttc acgtaggggc ggtcgctgcg ccaggcccgc tcgttcatcg cgtggaggat
    11521 gcggcggtgc accggcttca ggccgtcgcg cacgtccggc agcgcccgtc ccacgatcac
    11581 gctcatcgcg taatcgatgt acgagctgcg catctgctct tcgatctcga ccgggacaat
    11641 cttctcgtgt atcgtttttc ctggttcctt gggcatatcg tgttcctttg cgcgtacgcg
    11701 cttactttac ggcgcccggc gccgcagcgc ccgggggccg gtcaatgatg cggatggacc
    11761 gcaaaaaatc aaagcactcc ttttccatcg cgggaaacac gcgctccggc gcattgatga
    11821 tgaccgtgag cccgctgttg gcgaacaccg ccgtcgccag cacccgggtg taggtcgtcc
    11881 gcacccgctt ctcgtaggcc gtcccgtaca gccccagcgc ggtgcgatag cccccgaaga
    11941 tgtcctcgct cgtgagcacc cgcgtcaccc gcgtaaaggg aaacgcgagc cgcttgctga
    12001 tcaaaaacgc cttcgggtcc acgggcgttg cgaaccgctc cacgtagacc accatgttgg
    12061 gcacgagcgc ctttcccttg tcggtcgtga gcggctcgcg cttgtagacc cgcagctcgc
    12121 cgttgggaag ctttttcttc tcgacgacgt tccaggccgg cggcagcggt atcttgatct
    12181 cctgcgccac cggcagatcg ggcttccggg tcggttccgt cgccgcgagg ggtcccccgg
    12241 acaggacgag caggaccgca ccgacgacga atgtaattcg accggccatc gccgccctac
    12301 aggtcgaggt tgcgcacgtt gcgcgcgttt tcctcgatga acttctttcg cggctccacg
    12361 gcgtcgccca tcagcacggt gaaaacctcc tcggcctcga tctcgtcgtc catgttcacc
    12421 ttgaggatgg tccgcttctc cgggttcatc gtcgtctccc agagctgctc ggggttcatc
    12481 tcgccgaggc ccttgtagcg ctggatcacc gtgttcttct tgtccagctt cttgagcacc
    12541 tggtcgcgtt cggcgtcgtt ataggcgtag ttcgcttcct ttccggcctt gatcagatac
    12601 aacggaggct gcgcgatgta caggtagccg ttccggatca gctcgggcat gtagcggaag
    12661 aagaacgtga gcaggagcgt gcggatgtgc gatccgtcca cgtcggcgtc ggtcatgatg
    12721 atgatcttgc cgtagcgcgc cttgccgatg tcgaactcgt ccccgccgat gcccgtcccg
    12781 atcgccgtga tgatggtctt gatctcttcg ttcgagagga tcttgtcgag ccgggatttc
    12841 tccacgttca tgatcttgcc cttgagcggg aggatcgcct ggaagttccg gtcgcgtccc
    12901 tgcttggccg agccgcccgc ggaatcgccc tcgaccaggt agagctcgca gagcgacggg
    12961 tcgcgctccg agcagtcggc gagcttgccg gggagcgaat cgccctccag cgcccccttg
    13021 cggcgcgtga ggtcgcgcgc cttcttcgcg gcgatgcgcg cccgggcgct cagcacgcac
    13081 ttgtccagga tgcgtcgcac cacctgcggg ttctcctcga agtactgcga gagatgctcg
    13141 ttggtgatcg actcgacgat ccccttcacc tcgccgttgc cgagcttcat cttggtctgc
    13201 ccctcgaact gcgggttggg cagctttacg ctgatgatgg ccaccagccc ctcgcggacg
    13261 tcgtcgccgg agagctgcgg gatcttcttc tcgtagccct ccttcttgag ggagtcgttg
    13321 atcacccggg tgagcgccga gcggaagccc accaggtgcg tcccgccctc tttcgtgttg
    13381 atgttgttgg cgtaggtgta caccgtctcg ttgtacgaat cgatgtactc gatggccacc
    13441 tcgacgtcca ccgtgtcgcg ctgcgaacgg aagtagatcg gctttttgct gatggaaacc
    13501 ttcttctcgt tgatgtactg cacgaaggag acgatgccgc ccttgaactg aaagatgtgc
    13561 tccttgcccg atgcgcggtc gtcgcgcagc acgatcttga tgcccgcatt gaggaaggcg
    13621 agctcgcgca gccgcttcga cagcgtgtcg aagctgtact cgagcacctc gaagatttcc
    13681 ttgtccggct tgaacagcac cctggtcccc tgcttcttcg ccgcgccgat ctgcttcacc
    13741 ttgccgacgg gcgcgccccg cttgtagctc tgggtgtagg tcttcccgtc ccggtacacc
    13801 tccaccgtga gcgactccga gagggcgttc accaccgaca ccccgacgcc gtgcaggccc
    13861 ccggacacct tgtacgactt gttgtcgaac tttcccccgg catggagctt ggtcatcacg
    13921 atctcgagcg ccgatatctt gtagaccggg tgcgtgtcca ccggtatccc gcggccgttg
    13981 tcgaggacct cgatggagtt gtcgttgtgg atggtcacga tgatggtgtc gcacaccccc
    14041 gcgagcgcct cgtcgataga gttgtccacc acctcgtaga cgaggtgatg gaggccgtag
    14101 acgtccgtgg acccgatgta catcgcgggc cgcttgcgga cggcgtcaag cccctccagg
    14161 acctggattt tgtcggcact gtagttctgg ctcattccgt tttactccag acggtgtcct
    14221 cgagcgcggt ccgcttaatc agggtaaagg gactcacgat gctgaacacc gtagtgttgt
    14281 ttcgattcac gatgaccgct ttgtgctccg gcgcggtccc gtccacgata tgcgcgttct
    14341 ccggcgaacg gcgcatggtc tcgacattga agatgccgat gatgcgtcgg tccgacacga
    14401 tacgcttgtt cccgatatgg atatacatgc cgcgctaccg cttcgcccca tcgccccagg
    14461 cgatccgctt cacctcggtc ttgattgatt tcaccataga acaccccatc tcctcgtcaa
    14521 gcgacttcac gatcgaatcg cgcatcatca tcacgtcgtt ggcgaaggtc gagtggtcga
    14581 cggcgatgaa gagcgtccgc ttgaacaccc gatccggccg gctgtgcgcc gcgatgatgg
    14641 cgcccaccac gcgcggccag gcgatgcgca ggcgctccag cgcgaccgcc tcgtccatgc
    14701 ccagccccgc gatcgcgacg gccatcaggt cggagacggg cacggtggcg ttgtgccgat
    14761 ggcgcgacga acgcgtcata gcgcgctcac ccgcccgtcc tcgacgcgga accgggccgc
    14821 gccgttcatt ccgtccccgc ccaggccgtc gcgggccatg gtcacgaagg tctggttctc
    14881 ccgccgaaag agctccagcg cggcggcgcg ccgttgcgcg tcgagctcgg ggaaaatatc
    14941 gtcgatcagt atcaccgcgc gtttcccgag cgcgcgctcc acgaagtcaa gctcggcgat
    15001 cttgagcgcg atcgccgcgg tgcgcttctg tccttgcgac gccgaatcgg cgaatcgttc
    15061 gccgcgccgg tcatgcagca cgaactcgtc ccgctggggc ccgatggtgg tgcttcccat
    15121 ctgcaggtcg cgccgcaggc agccctcgag tcgttcgaca atgtcctgct ttccagcctc
    15181 gccggtcgac ggcgcgtagg agagcgcagg cgcgagcgaa tcatccgcga tgcggccgta
    15241 cgcgtcctgt atcaccggtg caaacgttcg ggcgaactcc gtgcggcgcg caacgatcgc
    15301 aacggtcagc tcggcaaaca gcgcgttcca aaccgacaac tggtcgccgg gagcgcgcgc
    15361 ctcgccgcgg gcgtctttca ggatgcggtt tcgcgatgcg agcacttttc gaaacgaaac
    15421 gagatcgcga agatatccgc gatcgatctt ggcgatcacc gcatcgacat agcggcggcg
    15481 cgcctcaggc gggccgtcca ccagcgcgag gtcgcccggc gacaggatca ccgacacgaa
    15541 ccgcccgtag tactccgccg atccgcggat ctccgcatcg tcgatcttgt agcgcttgcg
    15601 cgcgcggccg tcgtacagcg cgcaaccgac ctcaaagcgc cgctcgtcgg gcccaacgat
    15661 gtcagcgcgg caaaaatacg aatcggcgtc ccactgcatc agcgccccgt cgccgacgcc
    15721 gcgaaacgag cgcaactcgg aaaggagcga cagcgcctcg atcatgttcg tcttccccga
    15781 cccgttgtgt ccggtgatga tgttcacgcc cggcgcgggc gagagctcca ggtttgtgta
    15841 gttcctgaag tgcttcagaa cgagccgctc gacaaccata ggcgtcccat gccggcgggt
    15901 cactccgact tgaccgacag cggcatgatc accgaaatgt agcggtcgtc gttttccggc
    15961 gcgatcgtca tcgggctttt gctgccggtc acgccgacgc gaaccacatc ggtatcgatc
    16021 tccttgagcg cgtcgatcag gtacagcgcg ttcacgccca actcgatggg gtcgccgggg
    16081 accgtctcga tgacgatgtt ctcctcggac tcgccgaaat ccggggagcg cgcctcgatc
    16141 gtgaggcggg acccttcgaa ccgcagaacg atcttgttca ccggctcgac ggtgaagttg
    16201 accgcgcgcc gcaacgattc caggagcggc tcgcgcgcaa tgccgatccg aaagcggtac
    16261 tcgcgcggga tcacctgcga ataattcgga aaggtcccct cgatcgtgcg cgtgacgatc
    16321 tcggtgtttc ctacgctgaa gaaacactgg ttcttcccga tgaaaaaccg gcacaccccg
    16381 tccgtcccga gaaccgagtt cacctcgcgg accgccttga gcggaagaat gaccccctgg
    16441 gcgatgtcgg acccgccgct tgacgacatc gtgttcatcg ccagccgacg cgagtcgctc
    16501 gccacggccg atatggtttt cggctcatcg gtcacgaaat atacgccgtt gaatacggcc
    16561 ttcaccgtat cggtcgacgc ggcatagatg acgcggcgta tcatgcgcct gaacgcggcc
    16621 tgctcgatct cgatgacgtc ctcgtcgcca tagcgcggta tttcgggaaa gttctcaccg
    16681 gacgtcccga tgatggagta ctggccgctc accttgcccg actgcgacct gatctgaacc
    16741 acgtgccgat cgtcaaccga aagctcaata acgccttcgg gaaggtcgcg cacgatattg
    16801 gcgaatcgcc gccctgagag catgaacgtc ccctctgtgt cggcgcttgc ggcgatcctg
    16861 gtgcgtatgc cgatctcctt gtcggtcgcc atgatctcga tctcgtcgtt gaacacgtta
    16921 aacaggcaat tcatgagcac ggcattgacc gccttggacg atacgacgga atcggcgctg
    16981 ctgacggcct tgaggagcgc gtccttgctt gcttcaatct tcatgatgtg caccacacgg
    17041 tattattact attatgtgaa tatataatac tattatatag ttgtagtagt aggcacggcg
    17101 aatatgtgga tacttcgcta actactgacg ccatcaggca ttccgcatat cgtccatgtg
    17161 gacacgaacg aggcggaggg cgaaaactct caatttaacg ccatgggtat cagacaacga
    17221 aaaaattatc aacaataatg cgacataata aacaatatac tcacacgtta tccccacgtc
    17281 agcgcgcggg tgtccgttac agttttagtt cgatgatgag ctcgtcgatc tcttctttga
    17341 ggttgggatc gtcctttatc atcttctcga cattgttcat ggcgttcagg accgtggaat
    17401 gatcgcgatc gccgaattcc ttgccgatct ccgtggtggt gaggctggtg agcttcctga
    17461 cgaggtacat ggcaatgaat cgcggcaaga cgatcttgct gtgacggccc ttggcgcgca
    17521 ggtcgtccac ggtcacgtcg tatttctcgg agaccttttt catgatgtcg ttgatggtaa
    17581 tcgccttgct cacctcggcg tcgaaaagat ccttgatgtg cgttttcgcg tgctggatgc
    17641 tgatgggctc gtttgaagtc tctgagacgt gccggagccg gttgagcgcc gattcgaggt
    17701 agcggatgtt cgatttgatg cgcgtggcga tgaaggtgat gacctcatcg ggcacgtgga
    17761 tgctgtcgct ctcggccttg ttgcgaagaa tggcctcgcg cgtttcaaga tttggctgct
    17821 ggatgtcggt gatcagtccc caggcgaaac gcgtcttcag ccggtcctca agcgtggaga
    17881 ggtccttcgg ggggcggtcg ctggatacca ctatctgttt tttgctctgg tgcagggtgt
    17941 tgaaggtgtg gaagaattcc tcctgggtct gctccttgcc ctcgaggaac tggatgtcgt
    18001 cgatgagcag caggtccacc tcgcggtact tgatcttgaa gctctcgagg gttttggtgc
    18061 gcagcgagtt gatgaaatcg ttgatgaaca gctcgcactg cacgtagagc actttgagaa
    18121 aggggcgctc catcatcacg gaatggccga tggcctgcat gaggtgcgtc ttgcccagac
    18181 cgacgccgcc gtaaaggaac agcgggttat aggtgttacc cggcgatttc gacaccgaca
    18241 ccgcggccgc gtgcgcgaac tggttgttcg gccccacgac gaaattgctg aaggtatacc
    18301 gcggattgag catcatggtg gacggattgt tgatggtcga cagggcgtcc ggaaccggga
    18361 tgcccgtctg cgcggcgggc gacggcgtgt ggccgtttgc aatgacgaac tcgcagaaat
    18421 actgctcgcc cgtcagctcg cgcaacagct ccacgatgag cggctggtaa ttgtccgcga
    18481 tgtgacgtcg cgagacatcg tccgggaccg tgatgcgcac cgtcttgtcg aggatcgatt
    18541 caaccgacgt gttctgaaac cacatgtcgt acgactgctt gttgatcttt gtttgtacgc
    18601 cggataacac cttgttccac atttcgtgtg tcataccttc cttgatgcgt tatcaacaat
    18661 tgttaataac tttgtttata aatggcatat atttgagtag tattcaccgt gtcatgatag
    18721 aaaacgcgcg gtccccagta ttgtctctcg attggggaac ccgaaggatt atcctctgca
    18781 attattttcc aaagggcttt ttggcaagga ttttatactg ccgcacaaat cataggcatg
    18841 tttttttccc gcatgcggat agggtgcgga tatcctgtgc acagccgcca gatcattgtt
    18901 gataacaggc gcagagatgt tgatatcgtg tgggaagcgg tggacagatg gaagatgatg
    18961 ccgcacgcga tcggccggat catgccggcc gatcgcgaac gttgtgcgct acagttgatt
    19021 gaagttctgc tggtatccgg aattgttcgg ctcgcgcttc agggcctcgc cgtaccagta
    19081 cttcgcctgc gcattgtcct tgagtttgtt gcgatagatc cacccgaggt gccccgccgg
    19141 ccaggccttg tcccggtgct gattgtggca gtcggtgaga tacttcaccg cattcttgaa
    19201 atcgccgacc tctccgtagc aatacccgag gttcgagagc gtgaagtagt aggtggggtt
    19261 atgcttgagc gcgacgtgat agttcttgat cgcctcgcgg tagtcctttt tcactaggga
    19321 gatataggcg atctcgctcc aggtctggta gtcgtccgat ttcaccttca cggccttgcg
    19381 aaactgcgcc atcgcctcgt cgagacggtt aagacggcgg tagccgatgc cgaggttgcg
    19441 cagccccagg atgtaggtcg gatacttctt caggacgacc tggtacatct ccagcgcccg
    19501 ttcgagctgg cccttgcggt cgtacacgac ccccaggttc atgtatccgg tcttgaactc
    19561 gggatcgatc tcaatggtgt tcttgaagta cttctcggcc agatcgagct tcttctggtc
    19621 gaggtagatc accgcgaggt tgttgtgcgc gtcggtgagg aacttgttct tctcgatgga
    19681 gatcagcaga tacttctcct cctgggcgtc gttatagccg tagatacggc tgagcagaaa
    19741 gtacgcctcg gcgttgttcg ggttgatctc gatcgccttg cgcgtttcgg cctcgcagag
    19801 cttccacttg ttagggacct tcttgtcgtc gacctcctga tagtagcgcg ccagggcgat
    19861 gtgcgcctcg tcgaggttgg gcgacaggtc gagcgcccgg cggctgttct ggtacgattt
    19921 ctcaagcagc gagtcgtcga tgcggttctc gtagttcttg agctcccagc ggcgaagcga
    19981 gtacgccttg cccagtcccg agtaggccag gctgtagttt ttgtccacgc cgatcgcctt
    20041 gtcgaaccac ttgatggcct cgagatagtt gagctgatag agcaggttgc tcatgccctt
    20101 cacgtagtat tcgtaggccg agatgttctt ggtgaagttc tcggtgatgc ggtttttcac
    20161 gtccgccgtg atcttgatgt tcgtctggtc gatgatcttg agcgcgatct cgtcttgcag
    20221 ctcgaagatg ttgtccatca cgccggtcga ttccacctgc ttgaggatct tgtggctctc
    20281 cacctccacg agcttggcgg tgatgcggat ccgattgccg tatttctgga agttgcccac
    20341 gacgatgatg tccgcgccga gggccttgcc ggcctgcttg gccttcttgt cgtccaggac
    20401 cccggtctgg ccgagctgca cctcgtggat gatcttgtcg acgttggtgc gctccacgac
    20461 gatgtagtcg cggacattgc gcagcttgtg cgagaccgaa tcggcgatgc cgatctcgag
    20521 ccactggaca gccttttcgc ccgcaaggtt cgtgaaggcg gacacgccga tcacggtctc
    20581 ggcgacaagc gccgccggga ccagcaggag caggagcatg atgagcgccg tattttttcg
    20641 ttgcattgtt tcctccgatg aaagatcgtt cgccgatgcg gccgcagcgc agtgtggcac
    20701 ggcgacgcga tcctgtcaag ccgcgattgc cgcgcgacat gggtgtcgta tattcgggaa
    20761 aaggtacttt ttcaagaaag aaatggcatc gacatgaaaa ttcttttacg cggcggcagc
    20821 atcgccgcag ggaccggggt tcgcgtcggc tatgccgaca tcctcgcgga gcggcttcgc
    20881 gaacgcggtc tatcgctcat caaccgttcg cgggcgcgcg acagctcctt cgagggaaac
    20941 tggaccttct ccggggacat cgatccggtg cggccggagg tcctcatcat acatttcggg
    21001 atagacgaca tctaccgccc cgtgtaccgc tcggagttca aggagaacct cgtgcagctg
    21061 gtgcgactcg cacgcgcgcg gttcgatccc gcggtgctgc tgtgcacctc gcatacctgg
    21121 gacgacgagt acgagatgcg cgcggcggac atctactatc gtacggtgcg cgaggtggcg
    21181 ctggacctgg cctgcgagta cgtgcccgtg cacctggcgt ggatgagccg ccttttcgag
    21241 atcggaaggc ccccaaagga ctttctgctt gcggacgacc gccttccgaa cgaggagggc
    21301 caccggctct atgcaaacat actatggccg cacctggaaa aattcctggc gccgtatttt
    21361 taagtggaca gtccggagcc gccgaacaac gatctccgcc gcgggaatgg ccgcgaatca
    21421 gaaaaaaccg ggagcgtcac atgagcgtca tcatcaagga catacgcaga aacgatcgcg
    21481 agatcatccg catcgaggtc tcggaattca agggacgcga cctcatcaac ctccgcatct
    21541 ggtaccaggc ggtcgacgag cgcgggaatg tcgtgtacaa gcccacccag aagggcgtcg
    21601 ccctcgacgt atcgaactac gccgaactcc tcgacggggt cgagcgcatc gggaactatc
    21661 tcaaggatcg cgaggccggc gtcaatccgc tgggtgaatg atcccctcct cgacgaggcg
    21721 ggcgatggac gcaacgcaca gcgcccgcgc cgcgtcaatc tgaagctcat cgtgcaggat
    21781 gtccgggttc gccacgccgc gaagcacgtc ctcgatcagg aagcgcggcc cggaggggag
    21841 cccttcgacg aggtccgtcg catagcgggc ccgaagccct ttgagcgcgg ttgacaggac
    21901 aaaccagccc gccgacacga ggacatgccg gaagtcgccc ggaccgatgc gccgcaggag
    21961 atacggaagg ctctccgctc ccagaccgcg gcgccgaagg cgctgccggc ggtactcggt
    22021 cacgagtgac gcgtgcgcgg acagccgctc gagcggcgac ggcgcgatcg aaccggcgcg
    22081 ggagagcagc gactcggtgc tgcgtcggga cacatgctgg ccgatgaggc ccccgtacca
    22141 ggcgggatcg tcggcgatgc agcgcgcgac cgtgtcctcg tcgcaggcgg acaggaccgc
    22201 gtacagcagg ccgtccgcgg cgatctcgag gaaccagtcg cgaacgtccc tcgtcaccgc
    22261 gatccgcagc gatgcgagaa gcgcccgttc cgcctgaacg gcgcgcagaa acggcgcgaa
    22321 accgacatca tcccccccgc gcgaaaggaa caggaccgct tcttcgatgg agtacgcacc
    22381 ggccatgaga tcgcgccgcg tgagccgctc gaggccgcgg tagcgccgca tcagggcgcg
    22441 cacctcgtcg acgaggcgcg gcgagaggtt tcgccgtatc cgtcccgcct catccggaag
    22501 cgccatcgtc atcaccatga gttggtaggg gccgatgagc cgtcggttca tgaggcggta
    22561 gaggagcgac tgcagggcgc tgtcggaaag cgaagcgacg aggccgggca gatggggcag
    22621 caggcgcatg ggctcgtcga aaaaggcgag gaccgcatca ttgagcgtgt ccggatcgat
    22681 cgccccgggg ccgatgaaat ggcgcatgaa cggtgccggg agcgttattg aacacccggc
    22741 gtccccgtcg gtagagacca cggagagatc gatgcggaca gacgggtccg tgtccccggt
    22801 gtgcgcggtc ggtccgcgtg gagcgggatc ggccgcaacc gtcatgcggc gcttgagcag
    22861 catgctcaga agcacggacg agaggccgca ggcgtcggcc gtgtggcggg aaagggattc
    22921 gaaaagctcg ccgggcagat ccagattgcg tatctcgatg cgatggacgg ccggcgcatc
    22981 cgtgacggac agatgcatgc tcgccgcggc gccgggctcg gcgcgatcgg actggaggcg
    23041 caggggagag agcgccatgg tgcagcccgg acgcgcgcgg cggaaaaagt cgtcggtgtc
    23101 gggcaggagc agggcgatga tgagtcggga ggcggcggtt cccggcaggg tcaaaactcg
    23161 atgctcagaa aggcgatgtc gtcctttcgg cggtccagcg acgtgaacga ttgcaattct
    23221 gcgagcagcc cctcgcagaa gtcgtcgccg tgcaggtcgc cgcgcgcctt catgaaggcg
    23281 ccgatgcgtc cctcgccgaa ctcctcgttc tcggcgtttc gcacctcgga gatcccgtcg
    23341 gtgtatagga agaggcggtc gcccggttcc acgcgcaacg ccttctcgcc gtagttcgcg
    23401 tcgtcggcga tgcccacgaa gaggccgtcg gtgtccaaca ggagcacggc gttcttgctc
    23461 tttcggaagt acagcgcgcg ggtgtggccg gcattggtga agtggatcgt gttctcggcg
    23521 ggatgataga tcacgtaaaa cgccgtgatg tactggttgt cgagcaggag gcccttcatc
    23581 tcgcggttca cggcggccag aagctcgctc gttgagccga tgttcgcggc gtagatgctg
    23641 aagatcatct tggacatgga catgaccagg gccgccggga tgccgtggcc ggtcacgtcg
    23701 gcgatgagca tgccgaccga gccgtcgggg agcgtgtagc agtcgaagta gtcgccgccg
    23761 atgtcctcga tcgggagata ccgtccgctg atggtcggat aggagatctg cgcaaagctt
    23821 ttcgggatga tgaactgctg gaccttcttg gcgatctcca gctctttttt gatctgcagg
    23881 tttttcgcga agagctcgtc gtgggcgcgc ttgagcttga gcagcgaatt gacccgggcg
    23941 accagctcct tctcgttgag cggcttgagc aggaagtcgt cggcgccgat ggacagcccc
    24001 ttgatcttcg agttgagatc gtccgagccg gtcatcagga tcaccgggat gaactcatcg
    24061 cgcacgtggt ccttgaagcg ctccaagaac ccgaacccgt caaggttggg catgatgatg
    24121 tcgaccagga tgagatcgaa ccgccgccgc gcgaggatgt cgagcccctc ctggccgtcg
    24181 gccgcctcgg tcacggcgat gttcatgcgg tcgagcgttt tgcggacgat ggcgcggttc
    24241 agacgggtat cgtcgataac gaggatttcc cagggggtct ctttctggag ataatcgttg
    24301 atgagcatgg ccatgcgtgg taccgttgtg ctcccgcgtc gtgacggtgc aaccgtagtc
    24361 gcgtaaaacg aaagttcaat taaaatattg cgccggggga aaaaaacgaa cagccccctg
    24421 gggattgtaa ttgacaaaga ccgcccctgc cgaggcatgc tgtcccgtcg cacaaatgat
    24481 tgaatcgagg ggtgcaccgt gagcaaacag tacaaccccg gcgaaaagcg caagcgcgcg
    24541 aagcgccggc tcaagcggaa aaagcagaaa caacacgagc agcgcaagca gggaaaatag
    24601 cgaccgccgc atcagcgccg gataatgatg ctgtgccgct ccgccgtttt caacgcaacg
    24661 cagaatttta cgaaatcttg atactcgccc ggctcgatac gggcgcgcga aaagcgaacg
    24721 gatttcacga cccgcagggt gcgtgaaccc ttcgcttttt ttatgtcgat gcgggcgtgc
    24781 ccgtggcggg ccgtgagccg cacctcgcgc tcgacgacgg cgtcggcaaa tcgatcgggc
    24841 agggtgaact cgtaagcgtc gtcctcgctg atggcgtgcc ggatgaccag gggcgtctcg
    24901 cgccgttcgt agtggagata gtcgagcacg cggctcgact gcgacatcgg ctgcaggatg
    24961 atgcggtcgt ggccgaccgt cgcgaacgag tcgcagcggc cgcgcgccgc aatcacgaac
    25021 ggacgtgagt attcgcgaag gttcgacagg cggaactcgt cgatgctcat cgaggggaac
    25081 atcccgccca ggacggcgtt gacgcgatcc tcgcggtagg tcgcattcat gaaatcgcgc
    25141 cgcatcgacc cggtacggcc ctcgtaggcc acctcggcgt cgatggcggc gtctcccgca
    25201 tccgagatcg taatgcgata cctgcccgca atcccgccgg gcgtcgtgtc gcggacggtc
    25261 cgatgctcga acccgttttt caaaagcacc agggcctcga cgcccgccag ctcctcgggg
    25321 accgccccgc agggggcatg gcggccggtg aaatcgagcc acaacgactc gcgcccgtcc
    25381 agcgggacgt agaggagcgc gtgggtgaac gcgtccgggg acatgaaggc gcccgtcggc
    25441 ggcaggtgcg catcgcgcgc gacggcgatg aaggaccgaa tccccagtcc cgccaggagc
    25501 gatttcgcga gtaccgcctt gtcctccggc gtgccgcgca tccgcatgag ggtgttttcc
    25561 gccggctccg ggtggtacag aacattgcgc agcgtgtcca tctctcgcgc cgtgaattcg
    25621 tacacgcgat cgaccgtttc gcgcagcgtg ggggccgcaa aacgacgcgc cgccgcgggg
    25681 tcgattcgct ccgcgttcgc tatgatgcct ccgtaccagg tgacgaagtc ggaggcgtcg
    25741 cgcatgctcg aaaaagcgta gtgcggaagt cgcccgatgc cggggccgct gtcgtattcg
    25801 cgccggaccg ccgggaggtc ggtgagcgtg atcgcgtggc ggacgccgcc ggcgaccgcg
    25861 gtgcgctccc gcacgccctc gcggttgcat acgagcgtga tctcctgtcc tgcgggggac
    25921 gtgacgctga tatcgcagcg gtccaccggc tcatcgaacc ccgacagcgc cgtcaggggg
    25981 atggagaaga agctcgaatt gcgcggctcg gtcaccggaa aatcgaccac ataggcgata
    26041 tgcaccaccg agccgatcga gagcgacgaa agattgagat aattggtccc gttgatcttc
    26101 tcgatgcgat gggaatcgct gaagctccca tcggcgtggt acacccgcgc ccgcaccggg
    26161 tggagcgttc ccgcgaaggc gatctcgtac tcgccccagc gttcgagctg cttccggtct
    26221 ttcaccagat acacgtcctc gcacaagacg cggcttcccc cgtcggccat gaggtggaaa
    26281 aaccggccgc ggtataggat gcgcgagggg cgcgattcgg tgtcggcgga tacgccgcgc
    26341 aggatcgcgt cctcggcgcc gccgatcccg tttcgagcga acgggctctc gatccgctgg
    26401 cgcgccaggt acgacatgag ctcggtcatg gtgaacaggg acggcttgag cgcgagcatc
    26461 ttgtcccagt gcgccgacgg gtcggcgccg cgccggtagt agagctcgcc gagcttcagg
    26521 agcagatacg ggtcctcgcg gcgcgacagg gcgtcgatga tggccgcgcg ggcgccgggc
    26581 tcgtcgccct tcgcgatata cgcgtcgatg agctcggcga tcagctcgcc ctcgggatcg
    26641 cgcgatcgaa ggaggctcac gatctcctgg tgcgcgccgc ggtttttgta caggcggacg
    26701 agcgcggtga ggtcgctgcg gtcgttccga gccgcgagcg cgacgcgcag agcgtcttcg
    26761 gcggcgcgtg gatttttcgc gcggacatgg gacgcgaaca ggcggtgcgg cgcgggcgac
    26821 gacggaaagg ccaggcgaaa cgagcgaagc gcatcgtcga attctgtatc atatccgagc
    26881 tcggagagga agcgcgcatg ggcgacccgg gccggcagat agcggggcgc atcggcgatg
    26941 aggtcgcgtc ccgtgcggaa cgcaccgcga taatcgcggc gctcacgctc ccgctggaag
    27001 cgcctgaagc gcgccggcgc gaagtcggga tgctcgcgat tgagcgattc gtacagggtc
    27061 cacccctcgc tggcgagggc gctcgcggcg ttctccccgt aggcgatcag cgccgcggcg
    27121 agggtgaagc gatatacggg gtcgggacgc cgggcgatgg cggcgcgata gtgcgcgatc
    27181 gattcacggc tgttcagctc gtcatagtag gtgccgagcg cgaagtggga ctcggcggtg
    27241 tcggaccgga ggcgctcgac gagtgcggga aacgggacgt cgggctcctc gatgcaggcg
    27301 gcctcgtcag tgctcgtctc gtcgggacgg gccgtcgtcg gaacgatcgc gtcgttcgca
    27361 tcggtcacca ggacgcgaaa gcgccagtcg ccctttttca tcactttgag gagcagcgag
    27421 atgcggcgcg ggccctcgac gcggaggacg cgcaggtttc gcgaggtccc ggacgggtga
    27481 ttgcggaccg cctcgcggcc gttcacgaag agcgtgtacg ggccctcggc gtagacgcgg
    27541 accttcaccg gacccggcgc gacgatggtg gtcgccgcgt aggcgacgcc gtcatcggga
    27601 tagaggtagc gcgagaggtc caccgccccc gaggcggcat cgacacggac gatctttcga
    27661 tgcaccgatg cgtcgctgag tttgatggag agctcgggcg ggaaggggtg gcgaaggtcg
    27721 gcgtcgccaa accggtagta ggggccgatg agcgtccagg tgcgcacggg agacaggcgg
    27781 gccgacaccg cggcggcacg cgtgcgatcg aactgataga gcgcccgctc cagttcaacc
    27841 atgagcgtga ggcgcgcgat gttccggtgc ggccccccac cgcgctcgac cgccgcgatc
    27901 agtttctcca gcgtcgctgc gacgcgcgcg ggcccgtgca catcggcaag gcgcgccaga
    27961 tcataataat ataggaacga ttcgaaacgg tcgggaaacg ccgtgagcag gcgctcgatc
    28021 gcatcgtaca gcgcgacgta atcgcccgag gaaaacgccg cgttaacggc gcgctcgagc
    28081 tcggaaaagg cgtgcggcgc atcgggagcg gccgttgcgg cgcgggccgg cgatatcgtg
    28141 acgagcgcga tgaggaagaa cgtagcgagc gcgagaagga agtggcgcgc gcgacgatgt
    28201 atgttcatgc gattcaccgg gtgcactcca cgacatattt tttatacgta ccggacaggt
    28261 ccgacaggac gcgcgccgcc tcctcgaggg agatcacggc ctcgacgaag gggagcggat
    28321 cgacgagccc ctgcgcgaga agctcgatcg ccggcgcgaa cgggccgcaa cgggtgccga
    28381 tcacggtgat ctcgtccacc acgacgcggt tgaagtcgac cgtgagcgcc ccggcgaagg
    28441 tgctcttggc gaacagcgtc ccgagcggtt cgatatggtc gagcgccagc gaaaacccgc
    28501 ccggcgtgcc cgatgcctcc acgaccaccg gaaagcgcga ccggtcatcg ccgacgccgt
    28561 cgcgatgcga gcgcagcgag atcctcggat agcgctcgcc gaagatgcgc atcttgtcgt
    28621 cgtgctttcc gatcagcgtg acggcgttcc cgcgcagcga gagaaccccg acgatgaggt
    28681 acgagaggat gccgtcgccg atcaccagga ccgaaagctc gggggcgaac gggcgcaccg
    28741 agacgatctc gagcgcggcg gccagcggtt cgataaaggc gccatgcacc ggggagagcc
    28801 cttcgggaag gacgtataga ttgcgcgccg ggagcgtggc gtattcggcc atcacgccgt
    28861 ccttgcggaa gatgcccagc gtcgttcgag tggggcagtg atgataccga ccctgcgcgc
    28921 acgtcggaca gtcgtggcag gcgaggttga tgtcgccggt gacgctcgcg ccgacgagcg
    28981 acggatcgtc ggtctcgacg accgtgccga tgaactcatg tcccggaatg ccggtaaatc
    29041 gcatgtagcc gcgggcgatc tcgcgatcgg tccggcacac cccggcgtag cggacccgga
    29101 tgagggcctc cccggcggtg cggacgggct ccgggagatc ggtgcggtat cgcatcgtcg
    29161 ttccatcgaa gtggatcccc ttcatgggcc gttcctccgg atgatgatct tttcgttctc
    29221 cttccggttc acgaaacggg tgaaatcgcg aaaccgcgca taggacccga tgggaatccg
    29281 atgggccttg aaggcgatgt gcgatacaac ctcgatcacg ccctcgcgcg cgacgaaccg
    29341 gaatcgcgcc tcgcagtcgt cggtaacgaa ttgctcgctt ttcgggaccg agaccgggga
    29401 aaacccctgc ggcagatgat accggatgac ggtgtcgctg gagaaggtcg tggtgaacac
    29461 gacggggtgc gtccgcgagc gaagcatggc gtacgtgctg tagtagttcg atgcggagaa
    29521 aaacgggcca aagaccatct cggcgccgtc ggtctccagg aaggagggga ccgtaatggt
    29581 gtaccggtac accatcggct catcgggttc ggcggcgccg tactccgggg gcgaaatcgt
    29641 cgcgcccgga aagcgctcgt tccagtaggc cgagatcgac gcgcgcttct tctcgcggtc
    29701 ctggagctcg gaccgggacc ccaccgcgta tgatccgcgc ttcgagagcg tgcggacgat
    29761 gcgcgcatcg ccggcgggcg tcaggtacac gtcgtttgtc acatcgtcgc ggttctcggc
    29821 gtagcggcgg tgcgcggtgt cgatgaaccg gtatcgccga tcgcccacga cgaatgcggt
    29881 gattgcgcgg tcggtcgacg gcagctcgct catgccggcg agcttcgcgg tcccgtcgag
    29941 gaacaggggg ccgttcgcat cgacgtggca tatcgcgtgg ttgaaatcgc ccaggtaggg
    30001 gaccgcgagg ttggccgggc cgttgtcccg ggtgcggacc agcgccaggt tcgcgtcgat
    30061 tccagcggcg cgcaggaagg ccgcgagcac cagggagatg tccttacagt cgcccatgcg
    30121 ggtgtgatag gtctggtcgg catggcgcgg tcggatgcca ccgatgccca gctcgaagcc
    30181 cacgtagcgg atatgcgcgt tcacgtggtt gtagatgcgg cggatcgtat cgtcgcgacc
    30241 ggacgcgccg gccaccgctg ttcgcagcgc atcgcgcatc tcggcgctca tcgtcatgcg
    30301 gtccttcacc agcgaacggt accagccgtg cgcctccgtc cagtttcgga aggtcgtaaa
    30361 atagagcatc gggaccagct ggctgcggtg gggcatcagc gactccttct tgtacggggc
    30421 gcggtcgcga agatggagcc gatggacggt atcggcggga ccgaccgttg tggcaatcgg
    30481 cgatccgtcg atcccgcgca ggtgcggata gaccgatttg tcgcgcggat gcgcaaggac
    30541 gcagttgaac accagggtcc ggtgctcgcc gccgacggga atgcgctcgc cgaaatagcc
    30601 cttgtatatc tcccccacgc gactgatgcg ggtatagcgc agatcgatga tgctccccgg
    30661 ccccagcgag ggcaggggga tcaccttcgc cttcacgtca tagaagagcc ggctctcggg
    30721 atcggaaagg gagcggtcgt agaaggcggt cacggcggtg cgggtcccgt cgttgatcac
    30781 cccacagtcg atgtcctcga tggaatccgt ctccggtgaa tacacgatgt actgacgcgt
    30841 aaactcgtcg agggcgctcc ggtcgttgat gagaacggcc ttccggaccc ggtgctcgta
    30901 ggacccgtcg gggaggaccc gtatcatcgt ctcgtcgatg aggattacgg cggcctcgtc
    30961 tcggtgccgc gcggcggcgc gcgcaaggtc gcggatatcg tcgcggaccg catggccgtc
    31021 aagcggatcg ggccggtttt cgatgatggt cagatagcgc ttgagcgcaa cgtttccctg
    31081 gtcgtgacga agggcgcgcg caagatagtg acgcgcgagg gcgtcgtttc ccagccggtg
    31141 gtacaccagc ccaaggcgca tgagggcgcc tgcgtgctgc ggcgcgcgct ggaggagcgc
    31201 gccgagatag ggaaccgccg cacccggacc ctgggtctgc tcgatgatgt cgatcaggcg
    31261 aatcagcgtg ttcggatcgt caggatacag gaccagcgag tgggtgagga ccgacgtggc
    31321 gcggtccagg tcgccggtcg cggtgaggca gcgcacgagc cggtcgatca cgacgggatt
    31381 gccccgctcc cgattgtagt gccattcgat gctcggcagc gccgcggcgt tgtcgccgcg
    31441 cgagaggtgc atgatgccct caatggcgcg tcccgtcgag ggataccggg agcgctgcgc
    31501 ctcgcgcgcc agccgcaggc cctcggtgtg ccagcccagc gcctcgaaaa gccgcgccga
    31561 aacgatcggg tatttgaatg acgcccggtt ctcgcgaccg atcgcctcaa tcagcggcgc
    31621 ggcttcgtag agaaagccgg tgcggatctt gccgtgcgca agctccgtga gcgcctcgat
    31681 gtggtccttg tgcgcgcgca ggcattcgtg aagatgcgcc tcgcgttgtt ccgcggtctt
    31741 ttcgatcatc gccagatacc agcgcgcggg aaccggcact gcgagcgagc gctggaagag
    31801 cgcgcgcacc tcctcgatgg acttcccggc aagcccggtg gcctggagca ggtacccgag
    31861 gcgaaaggcg gccattggat cttgcgccgc gaaattggag agcccggcgg cggagggaaa
    31921 cagcgacgag gataccgccg caggagcgct tccgcgctcg atcgcggtgt gcggcgatac
    31981 gggtgcgggt accgctcggc cgtcagcgtc ggtcagccga agggaaaccc ggatgccgtc
    32041 tccatcggcg gcgccgatct tgaccagaat cgcgtgcgcg cctttcgtga gacgggcaga
    32101 gatccggtac tggtcgtgaa agaaggaatg ctcgcgccgg ttcgaaaaga cccgcgtccc
    32161 gtcgatccag atatcgcaat agcccgtctt tcccacgaag agctgatagg cgccgtcggc
    32221 cgggacggag cggacggtcc gcagcagcac aaaggtattg tcgatgtcct tgaaatagtt
    32281 gttgatggtg aacacgccgg tgagtcccgt gcgggcggga aaccagcgcg cctccccgga
    32341 gcgcccccgc gatgctggcg gatcgaacgg gcgctgcgcg ttgaccggca tggcattgaa
    32401 gtcggcgccg ccccgattat ggaagggacc aataacgtcg cacgaggtca ggaagccgag
    32461 gccgtcgatc gcactcgtca cggcggagcc tgatccgcgg cgaagctcga gctgcgcgcg
    32521 gagcgtggcg gcgcgcgccc ggagaacggc gctggcgagg agcgcgggag cggccgactc
    32581 ggcgcgcgcg agggcggcga tggcggaatc gacgagctcg acggtctcgg caagctcgaa
    32641 gaggcgcaca agctcgattt cggcccggat gggatcgtcg tgccgcatga tgcgatcgct
    32701 cgcgatctcg agcgcgcgat cgtaattccc caggcgcttc tggcggaagt attcggactc
    32761 gtcgtcgggt tgggcgtggg ccacagcgcc gtgcgcgagc gcgatcgcgc agagaaggac
    32821 gacgatgcag gcacgggcga tgtgtcgtgg cggtcgcggc atgatagagc ggtgcgttcg
    32881 aacgcgtctc cccgggaaaa aattatgttt gaatagagcg gccgggcgga taatattcaa
    32941 ccataatttc tttacgatca tcatcgagcc atgattactc cgcgaacagt tcgaatcgtc
    33001 cttcttgtgg cgctcattct cgcgctcctt gcggtcatcg gacgcactgt gttcgacggc
    33061 gaccgggaat cgcgcggtgc gctcggcaac ccgttttacg atatcttcta ccggtactat
    33121 tcagggaggg aggatgtcgc gaagcgggac ctgcgcgcgc ttttcggaag cacgcgtcat
    33181 ggggcgcatg cgctcatcaa tgacggcgtg ctgcgcgcac gggaggggga tgccgatggc
    33241 gcggcgcggt cgttcggaca tgcgttcgat cgcggcgcgt cactcgcgct atcgtatcta
    33301 tcctcgcttc gggcgcggac tggtgcgggc taccgggaat ttctcgccga gaatccgctg
    33361 gtgcgcgaaa cgcagtgggc cgagtacgaa ctggcggtcg ccgccgcgtc ggagcggcgc
    33421 gatcccgacg ctcgctctca tctcgcgcgc gcgatcgcac ggggttttgc atccacggag
    33481 ctcatggacc aggagccggc cttcgttcgg atgcggaatg atgatgcata caaacggttg
    33541 cgcgaagctg cggacgcgaa tcgagcgtca cgaatttcgc tctcacggct catgcgtgcg
    33601 gagcgtgcga gaacgggcgg cgggaaggcg tcgtatgttc ccgaggcgat tgaaacggcg
    33661 aatgcgctcg agcgcgtcgg caggcgcgcc gaggcggagc aggcgctcct gcccctgacc
    33721 gcatcgagtg cgccgtttcg ggaccgcgcc atggcgacct atcggcttgc gcgtatccgc
    33781 gcgacaatgg gcgatcgcgc gggctcccac gcctatctgg aacgctttcg atcaatgatc
    33841 gcctcgggcg aatccgatcc aactggcttc aaggattgtg ccgcgcgctt ccaagatgac
    33901 ataatcagga atgatccgct cctccgataa tttttccgtt tgtttaaaaa atccttgaaa
    33961 tatttcaaat tcgccgtgag cctacgaaac ggaccgctta acctttcgcc ttcaggagaa
    34021 ttgccatgaa gaccgcacaa accgcattca tgctattgat gataaccgtt ctcgccgggg
    34081 ccgtatcgtg ccggctcaag gtgccgctca aggagatgtc tctagcgaag agcgccatca
    34141 cccgcgcggg cgaggtcaag gcggacaagt acgcgccgga cgacctgaag cgcgccaagg
    34201 agttcctctc cctctcgcac gccgatatca agaaagaaga cacctcgaag gcggaggaga
    34261 acgcgaagaa atcgctcgcc tacggcaccg acgccatcaa caaatcgctg ccgctccttt
    34321 cgaaggacac gctcgacgaa gcgaaaaagc tcatcgccga cgccgagcgc atgaacgccg
    34381 agcgctatgc gtcctccgat ttttcggacg cccggagcaa ggcgggcgag gcggaaaagc
    34441 ttcatggtga taaatcgttc tgggaatcgt atctcgtatc gaagaaggcg atcgccgccg
    34501 cgaccgcagc gcgcgacaag gccgaggcgc gcatccccga attgcaaaac cgcatcgcgt
    34561 ccatccgcgg tgaagccgac aacctgaagt cggcgcgcgg atcggagttc gcatcatccg
    34621 aactcgcgca ggtgtcgtcg cggctcgatg cggccaccgc cggcatcggc cagaagaacc
    34681 tgaaggacgc ctcccgcgcc atcgacgatg ccgagagcgc ccttaaccgc gcgaagggca
    34741 acaccaagcg cggcatcgcc gccgagaaga tccggaacgc cgaaaaggag ctcgatatcg
    34801 caaactcctc gaagatgaag gaccagttcg cggcggacat tcagcgcgcc tcgacgctga
    34861 tcacgaatgc gaagagcgac ctgtccagcg gctcgttcga cgccgccgcg accaaggccg
    34921 atgaggcgac cgcactcctg aaatcggcga attccaacat gaccgtaaag gcgcaggagc
    34981 tcaagaagac cgcccaggag aagctcgagg cggcggaaaa cgcgctcaag aagatcaagg
    35041 aatcgccgct ggcaagccac atgcaggccg atatcgacgc ggtgcagccg ctcatcgacc
    35101 agagccgcgg attcctgaac aacgacgcct atcaggactc catcgcgaaa tccgacgagg
    35161 cgctcgcgcg catcaatcaa atcaacatcg ccatggaaaa aaaggccgag gagctgcgcc
    35221 tcaagggcga tacgtcgaaa gaggcgaaag aggagcaggt gtacgtggtc aagtacaacc
    35281 ccaaaaagcg agactgcctc tggcgcatcg cctattatgt atacaaggac gccaagctct
    35341 ggccggtgat ctatacggcg aacaaggaca agatcaagga cccggacctc atcttccccg
    35401 gacagaagtt cagcatcccc tccatggaca agaagtcgga tgatgcaggc aaaaaggaga
    35461 agggaaagcc gcccgtcgag ggtacggtcg acaagcggga ctgatcgcac cgatagcgac
    35521 cggtgaaccg ttcggccctt ttctggggcg cactcgttct catggcggcg gtcccgtggc
    35581 gggccgtcgc ccgagacatt aacctcgacg agatctatct gcgcaagtcg tcgccggcgc
    35641 tcaagaaact catcctttat aagctcgacg cctacgagcg cgtcaacgcg cacttcgtcg
    35701 atcgcgacgc catcttctcc gcgtggacgt ccggcgacca gatcgtctac atcaaagaac
    35761 tcccgggcgg gagcaatgtc gtctaccgtt acaatccgca tcggcgtcgg ctctccgagg
    35821 ccggtcggtt ttcgggcgtg gtcacctatg cgcgcctgag cgggcgcggc cggtacgtgt
    35881 acctcaagcg gttcgtgtcc gatcgcggca ttataccgcg acccggcatg gtcattatcg
    35941 atatcgccgc cggccgcgtc atcgagcgga aatcgaactt cccctttctc gatttttctg
    36001 tcgcggcggg atcggacgcc ctctattacg aaacgaagcg cgggatcgtc gaataccatc
    36061 cgggcggcgg cgaggggcgg atgatgatac cccgcgccga gtacggggac atcgccgtgt
    36121 cggacaatcc gtccgtcggc tatctctcgc ccaatctcca gcgggcgctt gtcgtcaaag
    36181 gcggcggcgg gagctatcgg gcgaagctgg tgc
//
LOCUS       DAORHF010000007        32742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_755_length_32742_cov_6.011534, whole genome shotgun sequence.
ACCESSION   DAORHF010000007 DAORHF010000000
VERSION     DAORHF010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32742
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_755_length_32742_cov_6.011534"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PKJ16_01315"
     CDS             <1..314
                     /locus_tag="PKJ16_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45651.1"
                     /translation="EQSGWYAQLAVKPFLRWRFGARYDALNLNRVVIDNETQDTKNDF
                     SRASAMIEFVPSEFARIRLQYNYDRSRYETVEEVTRLKTVHEVILQFTMAIGAHGAHA
                     F"
     gene            328..1218
                     /locus_tag="PKJ16_01320"
     CDS             328..1218
                     /locus_tag="PKJ16_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNV45652.1"
                     /translation="MKRMTITLLFLLLGATWGEAKLNVVATYPWIADIAGRIGGEYAS
                     VQSLARGNWDPHFVAPKPSLIATVRRADLVIINGAELEIGWLPPVLRESRNARVQVGT
                     KGFLDLSAHVDLIDVPKNVSRSEGDVHPSGNPHYHLDPRRVPALAAAIRTRLCSLDRE
                     RCGAYEKNYTAFAADWHKRCARWEQALAGLKGARVVQYHRLFNYFFARFGLTRIVEIE
                     PLPGIPPSSGHLVRVIDTVKRQGAAFILTDVYHSREPGRLVAEKAGVRLVVLPHDVGA
                     VPEATDIVSLFDEMVRRLAP"
     gene            1215..1991
                     /locus_tag="PKJ16_01325"
     CDS             1215..1991
                     /locus_tag="PKJ16_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV45653.1"
                     /translation="MIELLAPALLVSLVLLGIHSYFGIRIIRRNIIFTDLAIGQMAAF
                     GAAISLFVLHGEYLYPISLAVALVAGGIIAVMARRTAHLEAFIGLCYAMGLSGVFILL
                     AKSPHGMEEFQNLMAYDILYTKPGDIGMVAALYAAIGAALVVIEKKTDGFANELLFFL
                     TFAVTVTSSVKMAGVLVVFAILLAPAYIAIQLAERERVPAFMKRYPLIVAWIVGTLIN
                     TAAIAVSYRFDFPTGYTVVFCNAFAAVAASFFAGGNSHRQ"
     gene            complement(2039..3919)
                     /locus_tag="PKJ16_01330"
     CDS             complement(2039..3919)
                     /locus_tag="PKJ16_01330"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV45654.1"
                     /translation="MREDLVKWKEAYPDKFKPEDEVFAHIRRGDRIFISTGCGEPQYL
                     VTALTAYVESHPKAFAEAEVFHVWTLGVAPYTDERFTYNFRHNSFFVGNNTRDSVNRG
                     LADYTPIFLSQVPALMRRRSIPINVALIQVSPPDSNGYVSLGVSVDISHAALECASVV
                     IAQVNAHMPRVHGDTFVHIGDIDFLVPHDEPLLEFAPQVPDEIANQIGAYVARIVNDG
                     DTIQVGYGSMPNAILHHLRDKRHLGVHSELLTDAMADLIRAGVIDNSNKSVERGKTVA
                     SFCMGTPATYRFIDDNPIINFQSIEFTNDPLVIAGIRNMTAINAALQVDLTGQATAES
                     IGSTFYSGIGGSSDFMRGAVLAPGGKTILVLNSTAQNGEVSRIVPFLDSGAGVTLNRG
                     DIRYVVTEHGIAYLHGKNIRERAMDMIAIAHPKFRPWLIEEAKRHNIIYRDQAYIPGT
                     KGEYPKHLEAYRTARSGLPLLFRPVRINDETLVKDFFYSLSDQSLQRRFMSSRRDVPH
                     NVRQSFVVIDYTHELVILACVERDGIEIVAGIGQTITEKETHTAEVAFAVRDEYHGQG
                     IGTELLSYLTMLAQKDGLQGFTADVLMENKPMLRVFEKMGFDMDMEIEQGAYHLTMRF
                     TP"
     gene            4075..5511
                     /locus_tag="PKJ16_01335"
     CDS             4075..5511
                     /locus_tag="PKJ16_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45655.1"
                     /translation="MRNGKAGEEPIITRCDSEIADPEVRALLDDERYACLRRYVVTNG
                     FRRFYVYYASGTSRAALVAPEAVVIDAQERYDRVTYVIQDDPWSLTDGRLGVRVSHDG
                     GETETYSADNINRLLVQGERMAFVNDFEGIVTLEGLAEVSRADNIAARSLFAELVKAE
                     YCAKNGALTSRQRRDIEEKGLIRFNWKRFGLIDAGAVAHAQARLQGAIMGCLRADNPI
                     AACYTTPAGLFNYDDSLIRFEPHLGRGEHTVYFNFLVGNRFGSFSPYSFIDVMGDIES
                     SKRVYAGLIEAGVIDRRGTLAEPGAVDDAGFDFIADADRRAGVRDIVRAARPLTLDDA
                     RRDIAESRERVTADLRRLRELSSAYEYTVASFSRFVDRLAGQRARAYLERYQGFRIVQ
                     EVADEIVKNRGIVLRDGAGDALLLLTDSVERSSPHYARLLAFFRGRLPDAKRISIAFA
                     YTNPPARVKRDMLHPAVQALHVMAQEEE"
     gene            complement(5519..6625)
                     /locus_tag="PKJ16_01340"
     CDS             complement(5519..6625)
                     /locus_tag="PKJ16_01340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV45656.1"
                     /translation="MRSGDDTSVSTGPDGASAPLDMNADLGLFYQLLQMVDGDWSDSI
                     QGICDHIRERFGALIVVLSVLDKTFNEFIYTAFTVSAETEQRLSRDGSRFTPESVLAD
                     IKRIYAENNAILDYGIYHGDILQSFASIYFRHDRERTVRMLTEMRMRTLFVIPLLETN
                     RNYRCTFHVITDHDYESGEKEFISQYSAQLNVALEIVFLVRELYTKATHDCLTKLFNR
                     KQGHLMLERDLERIPRNRQPLALAMMDIDHFKRINDTHGHPVGDEVLQATAALLSEGL
                     RKCDVVARYGGEEFMLILPDTDLPHAAEVIRRLREKIERASVATAAGPVSLTASFGIA
                     VTTHDRPLDASALIAEADRKLYLAKQNGRNRVEC"
     gene            complement(6634..7704)
                     /locus_tag="PKJ16_01345"
     CDS             complement(6634..7704)
                     /locus_tag="PKJ16_01345"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HNV45657.1"
                     /translation="MKTYKIAVLRGDGIGPEVVREGLKVLDAVSKREKFSLDLAEFDL
                     GGERYLRTGEVLPDSVIDELRRFDAIYLGAIGHPDVRPGVLEKGILLRLRFDLDLYIN
                     LRPVKLYENVPCPIVGKGPKEIDYVVVRENSGDVYTGAGGISMKGTPHEVAIQEMVYS
                     RHQVERCLRWAFALTQARNKRNTLALCGKTNVLTYVFDLWERAFHEIGAKDFPGIARE
                     YYHVDATCMHMINQPERFDVIVTGNMFGDIITDLGAVTQGGMGMAASGNINPEKTAPS
                     MFEPVHGSAPDIAGKNLANPLAAILSAKMMLEFLGEEAAGAIIERAVMKVVRSIHDVA
                     SGKMGKSTSAVGDEVADAAATL"
     gene            7789..7980
                     /locus_tag="PKJ16_01350"
     CDS             7789..7980
                     /locus_tag="PKJ16_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45658.1"
                     /translation="MKKSLTLLFLLIFSAQLVAIADAGMSVSAKKKNSSKHSLVKKQS
                     KKGKGTMSRGWKKTKKRGR"
     gene            8140..9339
                     /locus_tag="PKJ16_01355"
     CDS             8140..9339
                     /locus_tag="PKJ16_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45659.1"
                     /translation="MVATIPNTDTIRRGMRRIVVCILVAFIAFSATCSSRDVKRSAQE
                     EQYFTDLSNFLAGIPVPQQSRFWEATQHKFYAEHIKFMNNFWDRVMNDTVNRMVPWRE
                     AHMVRGFEKYPAFYPLSGADFINFYTIIPDAPEYVMVAMEHAGPAPEPLKLNPQQLRS
                     GLAALERVVFTYGYFNYFASRTMQKEMSNISITGATPVIMIFMARLGLRVTFVEPVGI
                     NNDGALEGINGAGAFANGVKPEYRGIRIEFRAPKDTKTRQLVYISMKMMNDNVQPTTP
                     SGRFFARYQNTRTMMKSAVYLCHYDKYKGVKEFILGKSIMLIQDDSGIPFRYFNNAQW
                     DIKLFGVYKPYFKLEGCHVYQQPELFESYKNNSSFPLPFHFGYGSVMGRERSTLILAV
                     RKQDKRA"
     gene            9386..11434
                     /locus_tag="PKJ16_01360"
     CDS             9386..11434
                     /locus_tag="PKJ16_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HNV45660.1"
                     /translation="MTAASVKKRLVSSVRRTLSLGDRLASTGKKTYALLVSLFAMVFL
                     YRVALTISLFVNDVKPFDFNPKLVPVWFMIGFLPYDLLLIGVIVLLSFAMSRLSRAEA
                     LAARPRLYYGVRIGGIVLTQALLVVLLTVHATHVRLLFEVQTGFEYYVVRELFFTASI
                     AGVLKQVPVAYYLAALVFIGVFWLIALLPHEAKVRLGRVLLIVMAVGLLLSPLSATLV
                     PTALVKEIRLNPVLFLLSDIAAGKVSSSAHHLAVDDADRKGMQLVSPEYINEPAEKET
                     KIIPLKSKKWNVVYFILESVGTRYVFDTKGGPAPMPFFQGLMKEGWYLKNHYAASNIS
                     SRATFSLLSGLYDYFDERVFCIEPHTTVPSIYSFVKDSHESFLVTPASTLWYFPDSFL
                     RNSGIREIHHLDNLTLRLTDQKHEKAGHYMARDEIQTVDFFIDRIKKAKGPFVGVYIS
                     FLAHFPYFDYGPAYRVRNNCDQLICKYLNNLALLDRMIQKLYDSLKEQNLLDSTILVI
                     AGDHGQAFGQHVQNNYLHYHYSYTENLQAPALLYQPDIFKPRVIETPTSHVDIVPTLL
                     DAMRVPFDPLLLAGESLFAKHLRRKYIFMYGQEKTISSLSADLIKVSFSLRDYRSWAF
                     DLKSDPGETRFLPLAQYEGQLAALKKFAGHTNASLKQYSAANREKKDFHGHRHPRVSL
                     "
     gene            complement(11451..12008)
                     /locus_tag="PKJ16_01365"
     CDS             complement(11451..12008)
                     /locus_tag="PKJ16_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45661.1"
                     /translation="MADMAMIGIPAALGAIALVLAIAGHIYTKRRRVFWDAILTESIA
                     YHRHHLEAGLARGEFTGRREERAREFLRVVTQQYPRERTRYRGLRDLPVMRLGVKSTL
                     VSLDELLFRIFREDPWGSCHRDDVLKKLIRTFQSTVYRSLFMHSLLGLPAILYMDGRL
                     GAARLGIARSGSARLYRDIADDLRR"
     gene            complement(12009..12323)
                     /locus_tag="PKJ16_01370"
     CDS             complement(12009..12323)
                     /locus_tag="PKJ16_01370"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV45662.1"
                     /translation="MAVDPTPYDRLAALFGALTSPIRLQILESLVDNHRKNPTWRCCV
                     SDIMSEIELPQPYISKHLKVLKECGILEYEKKGNRIYYSFAKNDVLDELIGYLRKYRS
                     CC"
     gene            complement(12323..12607)
                     /locus_tag="PKJ16_01375"
     CDS             complement(12323..12607)
                     /locus_tag="PKJ16_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV45663.1"
                     /translation="MIAFDASKCVGCGQCVKVCPQGVIVMRDGVAAITDVRSCMECGA
                     CRLNCDAGAIELTKGTGCLVAIIREDILKTVPKGTGCGCGTDTTKIPGCC"
     gene            complement(12604..13545)
                     /locus_tag="PKJ16_01380"
     CDS             complement(12604..13545)
                     /locus_tag="PKJ16_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45664.1"
                     /translation="MKDSFVALYEWFRAFRKTFSIEPGLYFIGERYDVAAPLLVTGNY
                     HLTVYLLWRVLRNRTVRILVIDTDGINVWCASGKGRFCAREILRQLRRYDRRLITRGE
                     RIELVLPKLSLSGVSLSELRKNGVSPRIGPVYRRDIPAYLDTAPLADRATDTYRFDLR
                     DRLFTLLPSLAQFMKFGLFVCAGLFAWDHWFATGIHWQVLPIIAVSIVLYVVLFPLLP
                     SRTFAIKGLSTAGLMIAGMALLASRNGSGIPDASTSAFYALFTAGANLFFALSYTGNS
                     GVSNYSLVKREIVRFLIPTIALGVAALVVIIVKGVMS"
     gene            complement(13699..15150)
                     /locus_tag="PKJ16_01385"
     CDS             complement(13699..15150)
                     /locus_tag="PKJ16_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45665.1"
                     /translation="MSIVPHLIHFGGIVGYPRDRLLRALNTDRPATGWDFLRKKYAKH
                     FGFGLEVRGALPPCGDVCDMASCAREALQLARGRYGAMLADADVHVRAGALPPAGDLI
                     AGYPVDLADGREYSKPVLIDIIHDAAARSYRIGAVAYDHIIDGAPLALLVQLMHNHIV
                     LRDDSLLARAGRSRETADRPPVPVRRGVLARERWERALAPSEESLFPVGGYLDVTDSA
                     VVALRSRIAREIGAAVPVSSVELALAAFETGLNWASDCVAQLAPGGIHDGYRGLGFVR
                     TSGYAQAAGLPHRAQYDWLRGVLAWASAETDRERARRGSSSLLYERYRRIPAFWVDLF
                     DRRIAYDDVMAVAGTQLLGSNLNGVDYGVPVHVGGIPAGALRYFFIPSHSEHHRETRR
                     ARAARNLETHVTEVSLSSAPFVTMGDGRRVMYKSTKASPATARRILAGWGRDDDARRD
                     GPAAVEALFRELYRPDRPSEGRIEQRARLLFAV"
     gene            complement(15288..16901)
                     /locus_tag="PKJ16_01390"
     CDS             complement(15288..16901)
                     /locus_tag="PKJ16_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV45666.1"
                     /translation="MTETRRTITLFALTMLMCIALASCRGGDGTDGAPVERWEILNDG
                     SRTHAQAASATGWRPISMPATFTIPGNRFDTMGHVWLRGSFDAGADPGRWYGISLGRV
                     YHADEVFINAQPIGRRTGTDLFRLHSPRNYAIAPGVIAPGRNEVSVRLGIFGREYGGF
                     AAEARILDGEAFAKRERQQHFWLTMIPFGIVFLYCGTIVLLLVFFGASRERKLLYSAL
                     GLAYYAAYILALFFPYPPRVIDCTTDQRLMGAMSFVLLSVPFFIVVLAFIVQAAYGRY
                     LTTVNRIALPALALLAAAQLINTYAVPGLVGRSGSYLINFLCIGLGAGYFCILLIRLH
                     ALHRDTTRIRILAPLLSLAIAMLAVENAIALAGHPGHGMIATYGSPIIVVLFMALFIG
                     DYARIKVEHARLYDQLQRPGRPPAGASDPASPDRPALTESAEEKLRRVIDFIREHYAS
                     DLSREGLAAAVDLNPSYLGRLFVSFTGEKIGEYVNRLRIEEAIRRLATRDARVIDIAL
                     SVGFESLSTFNRAFKRVTGTTPTEYQKRA"
     gene            complement(16894..17679)
                     /locus_tag="PKJ16_01395"
     CDS             complement(16894..17679)
                     /locus_tag="PKJ16_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV45667.1"
                     /translation="MRRRAIILILLLGCAGMASLGCVTIASLATRDFGEPLVYSGTRT
                     DFRILTTPPHPHNPVGGWPTMTVVIIDLPFSLVLDTLLLPVTGSWAVYERFFSSYNII
                     HYTRRTDVAMVKRALAQPLVNVHIADKDGRTALHHAVSTGSDELLGMLIAAGLRIDET
                     DRWGDTPLIEAASLCDCDAAKLLLDARADPNRANKEGHTALSRAKKRSGEHAAREREY
                     TARLRTIKQGSDEYLKSQVRKYRTQRELCDAFIRLLEERGARD"
     gene            17881..18411
                     /locus_tag="PKJ16_01400"
     CDS             17881..18411
                     /locus_tag="PKJ16_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45668.1"
                     /translation="MSDNTKQQRITFRRGIVATIIGIAHVVVGCAGTMWAIARIAVIG
                     YRAPLEMGAWMAAFNYLYEGHVALLWLSGIGLLRGRAWGRALALVWAALSIVLHVAAY
                     GIQRHYWGLLSPGFGWGDYFVLYYAGALVVAIAGSWALGRLPGWARALSELVGRDTPF
                     ARVLEALARPTERSKP"
     gene            18408..20045
                     /locus_tag="PKJ16_01405"
     CDS             18408..20045
                     /locus_tag="PKJ16_01405"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HNV45669.1"
                     /translation="MTPIGHMATGVAAGGIIAPVINKAFKTPYRALIPIYLVASVIPD
                     VDGLSLLVSRAAYFGKAWYSHHMAGHSIIGAAVYATVLALFYLLFAVTGRGVINLFRM
                     DKVPLEHRLRRTVGAWIAAFIGCLAHYAGDLPTPPGPWGGIALMWPSMTMYGGWNRIF
                     WHNWYLIYLSIGFLGSYLAVQLVAGFFAAFTPRVMRWTGYLFRTAAVVLCALFLYQTA
                     AFVHKHDLRTMGPARWDALNRSLVPAKYMKRADAYYDKATVFWRRSVITMPDIVKYGN
                     ITLHFFENLHRRVAPAVGRFMPVTRTREDDMALYRALQAMAPGMEDNRPGDYRVWILR
                     DALPRPDYFNRGFLYQLIHRTKKHIMQMTNAWMVVIHIEERDGAGNAVRVKKIYHNDK
                     IFVSDRIMPQLNLHELPQVTFKFWEHDRVQYSLIPGSDLTRFHNLAKGYYLTISTVRG
                     HLWPNWRSGVLIHDGPWTEGCIIHAYLHTDIGRHLEWAYYPLWERADEIIKKDIRGTV
                     YRGRNIYWGRLLFIKDPVRLETLAQRAPVTPQVAHSQ"
     gene            20134..21324
                     /locus_tag="PKJ16_01410"
     CDS             20134..21324
                     /locus_tag="PKJ16_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HNV45670.1"
                     /translation="MNRVRTACCIVGMLVVCAVPVRPDDAPNLRPLVEGNTQFALALY
                     KSLVVPGKNLVVSPYSVSTSLAMTSMGAWGKTLQEMHRALRLSMNSEAIHGSFAVLAD
                     ALEQRSGAGVRLTIANGIWLNKGLEVKKQFLTSAERSYKARADQLDFSGNAESSRREI
                     NEWISKKTAGRIAGLLPPGSVSPATSLVLANALYFLGNWEYPFDPKKTHTGKFIIGKK
                     RQVPAVMMRGKMLCRFLETSQAHIASLPYKGGALSLVVFLPSAKLGIEKFEKGLTAQM
                     MSQWLSRLQRREMEITIPRFTIAASLRLNETLKKMGMSSAFGPAADFSGIGGKLFISD
                     VFHSAYIEVNETGTEAAASTAVSMTKNGSRRAFRADRPFVYAIVDNATGSVLFMGRVY
                     QPKG"
     gene            21335..22099
                     /locus_tag="PKJ16_01415"
     CDS             21335..22099
                     /locus_tag="PKJ16_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV45671.1"
                     /translation="MLDVKSLRYHTEKDYLVLSIVFGMLYWILLIVLTFGIIIPIVAL
                     VALGLWIFEQFLKAMLFGDAIRVSENQYPELNAIVVDQCRELGMSNAPSVFIVNGQGM
                     INAMAVRVLSKRYVILLSDLVDLMLARDQIRELAMIIGHELAHHAAGHLDPKRNLAIA
                     PARFIPFLGTAYSRACEYTCDRIGYALIRDKAAAQRALIAVALGARTLASKTNIDAFA
                     AQEREVPRFFGVLTLIFSTHPRMTLRIRQLEGFEPK"
     gene            22195..22668
                     /locus_tag="PKJ16_01420"
     CDS             22195..22668
                     /locus_tag="PKJ16_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HNV45672.1"
                     /translation="MKRICIVALVAMVALVAVSCAKKKPSDIFTTLQKLGDTKEIADL
                     KQVYTAETVKAMEELMKIVPKDLQDSKGQNKFPKEAKWDVVSEKIDGDTAEVKVKFSD
                     HPMANMKGQEVTFKMKKEDGAWKIDLEKEMKAALALFQGLGGKDGLLKDLKKFIK"
     gene            22773..22856
                     /locus_tag="PKJ16_01425"
     tRNA            22773..22856
                     /locus_tag="PKJ16_01425"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22807..22809,aa:Ser,seq:tga)
     gene            22870..22956
                     /locus_tag="PKJ16_01430"
     tRNA            22870..22956
                     /locus_tag="PKJ16_01430"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22904..22906,aa:Ser,seq:gct)
     gene            22984..23499
                     /locus_tag="PKJ16_01435"
     CDS             22984..23499
                     /locus_tag="PKJ16_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45673.1"
                     /translation="MKKLTQRIDGKRDALKRTVAARRAQLQARIGEARAFAAGRIEFG
                     VRKFREYRTIAFLPENARFFAAAAYVPFVGWMLPLYLKEDSSFCQESGKQGFVLAVFF
                     AAIPMALSLLGVLFVPKDARIVSLVLAILVYLSNSVYLVLCVISAFMAIRTERLLAVP
                     VAASYAQRLQL"
     gene            23551..23624
                     /locus_tag="PKJ16_01440"
     tRNA            23551..23624
                     /locus_tag="PKJ16_01440"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23585..23587,aa:Arg,seq:acg)
     gene            23768..24247
                     /locus_tag="PKJ16_01445"
     CDS             23768..24247
                     /locus_tag="PKJ16_01445"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HNV45674.1"
                     /translation="MNDVHHEFMRAALEEARNAAEAGEIPVGAVIVRAGAVIARAHNR
                     NRRDRNPLLHAEMIAIEIACRAMGNERLIDCDLYVTKEPCAMCAGALVHARLRAVYIG
                     ARDVKYGACGTVLAVCGSPALNHRPAIEFGLLEQESTALLGGFFKRLRNHHTKERPE"
     gene            24244..24996
                     /locus_tag="PKJ16_01450"
     CDS             24244..24996
                     /locus_tag="PKJ16_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45675.1"
                     /translation="MNRRLSIAVLALIAALLASCATISYHDRMKYYGSPRSEFVDTTE
                     ARSMFEGVLVDDRLIRNGKPYYHLQFERVMDGAGRYLDLYLPHDTSDASLVFESNVKK
                     PGKRASFLLLERTCCSNEYGEFSDPLKTRDLEKNPERLFDIVRDKLKKNVTRADLPIV
                     FVKLVFSNVFKYDIIYTVWKAEGGDATSFSWNACLQLSYDTIDVKWKNRSRALYGLSK
                     VGYVGPVIVDIVTSPIQLIAAILYFGMGGAVK"
     gene            complement(25006..26823)
                     /gene="selB"
                     /locus_tag="PKJ16_01455"
     CDS             complement(25006..26823)
                     /gene="selB"
                     /locus_tag="PKJ16_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HNV45676.1"
                     /translation="MFIIGTSGHIDHGKTSLIRALTGIDCDRLPEEKQREMTIDIGFA
                     NIEYPKFGTVSVIDVPGHERFIRNMVAGAWGIDLALLVVAADDGWMPQTEDHFRVLEL
                     LGIERIIAVINKIDMVDDETIELVTADIAEHLAGTRYADADLARVSARTGAGVDELKQ
                     LILQNLGKLPKAGDAGRPYLYVDRVFTSKGYGTIVTGTLKNGRFHEEDEVTVLPQRRA
                     VRIKKIESHFQALAEGTPSQRTALNLAGIAREEIERGAIVARDNFFAESGDILTRLRI
                     LGERRIRNNIGIEVLIGTSTRKAKLILAHEPDDGSRTVIARIKFDEPWFFYPGQPFVV
                     THPGGHRIIGGGFVVLTEYGGLRNMRALRAKVGALAGFGREDLIAFSVAVRGAVPRAD
                     VINSLPDNPKAIEKIITTLINEKRITAQGPLLVDPAYGAASMAAIRDAVMGAVGLNIK
                     EIADRCGIDPEFCRLLMPAVAAEHGIVDKDGRYYAGNAVTPDTLSPDRKKVLDDVRAK
                     GREGVELDKADDAAKGRIRELVKLGFLVSLDGSIIFHREVYDELKRLVLAEFDRRDKL
                     TVPEAKDATGLSRKYIIPLLNRIETDGAIKRIGDFRIKA"
     gene            complement(26824..28209)
                     /gene="selA"
                     /locus_tag="PKJ16_01460"
     CDS             complement(26824..28209)
                     /gene="selA"
                     /locus_tag="PKJ16_01460"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HNV45677.1"
                     /translation="MSDDIASILRNIPQVEKILQDDDVARFVPVIGRDVTVAVIRDAI
                     DDFRRRVESGASGSAEELRGSIIGACGRKRLEKLQRVVNGTGVIIHTNLGRSPLRAEI
                     LQRLAAAMAGYCNLEVHLPTGERGKRGGFAEELACHLTGAQDALIVNNNAASVFLILH
                     AFAAGREAIVSRGELVQIGGGFRIPDIMRQTGARLVEVGTTNITELDDFKRAITPDTA
                     MILSVHQSNFRQKGFVRQPTLRELASLASDSVLFVRDLGSGNLIRDERLPLSFEPEVR
                     FEVAQGPDLVCFSGDKLLGSCQAGIIVGRRDLIAQLRKHPLMRALRVDKISYFILQET
                     LLAYMNERGREVGVWSGIFQDRAAVAKKIERTMKRITHERKKEMVARVECKSTFGGGS
                     MPGYTLQSVGVQIDVPGLRADDIDRALTANEVPIVGFIADGKYTLDFRTVLEEDIPVI
                     VQALTALFSGA"
     gene            complement(28264..28354)
                     /locus_tag="PKJ16_01465"
     tRNA            complement(28264..28354)
                     /locus_tag="PKJ16_01465"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28318..28320),aa:Sec,seq:tca)
     gene            28665..31079
                     /locus_tag="PKJ16_01470"
     CDS             28665..31079
                     /locus_tag="PKJ16_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV45678.1"
                     /translation="MDDGVNSQRFRLLFERARDAMCVVEDDVIVECNGVMLDLFRCAR
                     GDILGKCPADVSPALQPDGSPSRERMRALFDCALAGPAQRYEWRHERPDGSQFDAEVS
                     LTGIGFNGNRHLLAMIRTVSPCADADHAVRESKRKYRDLAEQLPVMVFEIDRFDGIVF
                     INRHILHTFGDRLFDQDNRLNYVEIIAAEDRAVFEGLLRRVNGGEEAHNVVLRGLTGA
                     GTPFPMQLSLVPVHDDGAIIGVRGIIIDITEQQAREDALRASEERYRAIIENIQDGYY
                     EVDLKGNFVNFNSALAHMLGIARGELMGVNYRAYTSPEIADTINRRFHEVFSTGTPLG
                     TLPWEISRADGTQRFLEMSVSLIYSPSGDPAGFRGIVRDATDRTRLLELIRASEERYR
                     AIIKNIEDGYYELDLRGNVTFFNESFRRIMEYPHEELLGLNYRRYTDEHTQRRLREAF
                     NAVYRTGAPNKNIEYDIVRKNGERRSVEVSVTLIFNAAGESIGFLGIMRDSTERKRAQ
                     EALRASEERYRTIIENIADVYYELDLTGTIVFINEPGLAMFNATREELVGSHFKRFSD
                     KNFVRTMYDAAARVLATGVPCSGIDWEITRTDGSTLTGNVSISLIRDANGEPAGFRGI
                     LRDTTERRRAEDMIKMLAYHDMLTGLPNRVLLNDRLAMSMNYAVRYHKKLAVLLFDLD
                     GFKEVNDTYGHQTGDLLLQKVSNRLLALIRKSDTLARLGGDEFIVLLNDIARTDDALA
                     FADKIVESFREPFHCSELDIRCNASVGIALYPEHGDDMETLLQKSDEAMYRAKRGGAG
                     KYELFH"
     gene            31210..31590
                     /locus_tag="PKJ16_01475"
     CDS             31210..31590
                     /locus_tag="PKJ16_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45679.1"
                     /translation="MKYVSGVVALCMVIGLAACAKGPVISLEKNELATSATSLKVLVA
                     NPVKGTDQSQKRYWVCLAKAGTPDSEWGDWSFVYDNMTAIDVAIKKNLVDGKLPAGDY
                     EVRLHSEYPTKPHNVVMRIKVAVK"
     gene            complement(31668..32741)
                     /gene="selD"
                     /locus_tag="PKJ16_01480"
     CDS             complement(31668..32741)
                     /gene="selD"
                     /locus_tag="PKJ16_01480"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018704231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(32667..32669),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HNV45680.1"
                     /translation="MKEQCSINAPLDAVRLTESVRGAGUAAKIGPGDLAAVMKQLVVP
                     VDANVIVGMDTSDDAGVYRLTDEIALIQTLDFITPIVDDPFVYGQIAVTNGLSDIYAM
                     GGRPLTALNIVCFPVSTFSLDTLARVLQGGQSVLDTAGVQLLGGHSVDDPEFKYGVSV
                     TGIVHPDRVLTNTGVRPGDAIVLSKALGTGIVNTAVKAGMADPAHAASAIRSMTLLNR
                     DAAACLEGRTVHACTDVTGFGLMGHLKEMIGNDPVAVHIDAKAVPLIPGAAEYASSGL
                     IPAGMYRNRDHVGALCTVDPSVPREVADIVFDPQTSGGLLVSLPAAEAEALCAALHAC
                     GCPDARIIGSVRESERPAIAITP"
BASE COUNT         5984 a        10117 c        10495 g         6146 t
ORIGIN      
        1 aggagcagtc gggctggtac gcccagctcg cggtgaagcc ctttctgcgc tggcgcttcg
       61 gcgcgcgtta cgacgcgctc aacctgaacc gcgtggtgat cgacaacgag acgcaagata
      121 cgaagaacga cttttcgcgc gcctcggcga tgatcgagtt cgtgccgtcg gagttcgcca
      181 ggatacggct tcagtacaac tacgaccgct cgcgctacga gaccgtcgag gaggtcacgc
      241 ggctcaagac cgtccacgag gtcatcctgc aattcaccat ggcaatcggc gcccacggcg
      301 cccatgcctt ttgacggagg tccgataatg aaacgaatga ccataacgct gttgttcctt
      361 ctcctcggtg cgacctgggg cgaggccaaa ctgaacgtgg tggcgaccta tccctggatc
      421 gccgatatcg caggtcggat cggcggcgaa tacgcgtcgg tgcagtccct tgcgcggggc
      481 aactgggacc cgcatttcgt ggcgcccaag ccgtctctca tcgcgacggt gagacgggcc
      541 gacctggtca tcatcaacgg cgccgagctg gagatcggct ggctgccgcc ggtgctgcgc
      601 gaatcgcgca atgcccgcgt gcaggtcggg acgaagggct ttctcgatct ctcggcgcat
      661 gtggacctga tcgatgtccc gaaaaacgtg tcgcggagcg aaggggacgt gcacccgtcg
      721 gggaacccgc actatcatct cgatcctcgc cgcgtcccgg cgctcgcggc ggcgatcagg
      781 acgcgcttgt gctcgcttga tcgtgagcgc tgcggtgcgt atgagaaaaa ctacacggcg
      841 ttcgccgcgg attggcacaa gcggtgcgcg cggtgggagc aggcgctcgc cggcctgaag
      901 ggcgcgcggg tggtacagta tcaccggctc ttcaattatt tcttcgcgcg cttcggtctc
      961 acgcgcatcg tcgagatcga gccgctgccg ggcatcccgc cctcgtcggg gcacctggtg
     1021 cgggtgattg acacggtaaa gcgccagggg gcggcgttca tcctcacgga cgtgtatcac
     1081 tcccgggagc ccggacgcct ggtcgcggag aaggcgggcg tgcggctcgt ggtgctcccg
     1141 cacgacgtgg gggccgttcc cgaggcgacg gacatcgtat cgctcttcga cgagatggtc
     1201 cggaggctcg cgccatgatc gaactactcg ctcccgcgct tctggtctcg ctggtgctcc
     1261 tggggatcca ctcgtatttc gggatccgga tcatccggcg caatatcatc ttcaccgatc
     1321 tggcgatcgg gcagatggcc gccttcggcg cggccatctc gctgtttgtc ctgcacgggg
     1381 agtatctcta tccgatatcc ctcgccgtcg cgctggtcgc gggcgggatc atcgccgtga
     1441 tggcgcggcg cacggcgcac ctcgaggcct tcatcgggtt gtgctatgcg atggggctct
     1501 cgggcgtgtt tatcctgctc gcaaaatcgc cgcacgggat ggaggagttc caaaacctca
     1561 tggcctacga tatcctgtat acgaaaccgg gcgatatcgg gatggttgcc gcgctgtatg
     1621 ccgcgatcgg ggcggcgctg gtcgtcatcg agaaaaagac ggacgggttc gcaaacgagc
     1681 tgctcttctt cctcaccttc gccgtcaccg tcaccagctc ggtgaagatg gcgggcgtgc
     1741 tcgtcgtgtt cgcgatactg ctcgcgccgg cgtacatcgc catccagctc gcggagcggg
     1801 agcgcgtccc ggcgttcatg aaacgctatc cgctcatcgt cgcgtggatc gtcgggacgc
     1861 tcatcaacac ggccgcgatt gccgtgtcgt accggttcga tttcccgacg ggctacacgg
     1921 tcgtgttttg caatgccttc gcggcggtcg cggcgtcgtt cttcgccggg gggaattcgc
     1981 atcgccagtg accgcgcgcc ggcgagcccg atccgtcgcg gcgcgcagcg gatcggccct
     2041 atggcgtgaa gcgcatcgtg aggtgatagg ccccctgctc gatctccatg tccatatcga
     2101 agcccatctt ttcgaagacg cgcagcatgg gcttgttctc catgagcacg tcggcggtga
     2161 atccctgaag cccgtccttc tgggccagca tggtcagata cgacagcagc tcggtcccga
     2221 tgccctggcc gtgatactcg tcgcgcaccg cgaaggcgac ctccgccgtg tgcgtctcct
     2281 tctccgtgat cgtctggccg atccccgcca cgatctcgat cccgtcgcgt tccacgcagg
     2341 ccaggatcac gagctcgtgc gtgtaatcga tgaccacgaa gctctggcgc acattgtgcg
     2401 gcacgtcgcg acgcgacgac atgaatcgcc gctgtaggct ctggtccgat agcgagtaga
     2461 aaaagtcctt caccagcgtc tcgtcgttga tgcgcaccgg acgaaacagg agcgggagcc
     2521 cggagcgtgc ggtgcggtag gcctcgaggt gcttcgggta ctcgcccttg gtgccgggga
     2581 tgtaggcctg gtcgcggtag atgatattat ggcgcttggc ctcctcgatg agccaggggc
     2641 ggaacttcgg gtgggcgatg gcgatcatgt ccatcgcgcg ctcgcgaatg ttctttccgt
     2701 gcagatacgc gatcccgtgc tcggtgacca cgtagcggat gtcgccgcga ttgagcgtaa
     2761 cgccggcgcc gctgtcgagg aaggggacga tgcgggacac ctccccgttc tgggcggtcg
     2821 aattgagcac caggatggtc ttgccgcccg gggcgaggac cgcgccccgc atgaagtcgg
     2881 agctcccgcc gatgccgctg tagaacgtgc tgccgatgga ctccgcggtg gcctgcccgg
     2941 tgagatccac ctgcagcgcc gcgttgatgg cggtcatgtt gcggatcccg gcgatcacca
     3001 gcgggtcgtt ggtgaactcg atcgactgaa agttgatgat ggggttgtcg tcgatgaagc
     3061 ggtaggtcgc cggcgtgccc atgcagaacg aggcgacggt cttgccccgc tcgaccgact
     3121 tgttcgagtt gtcgatcacc ccggcgcgga tgaggtccgc catggcgtcg gtgaggagct
     3181 cggagtgcac gcccaggtgg cgcttgtcgc gcagatggtg gaggatcgca ttgggcatgc
     3241 tgccgtagcc cacctggatc gtatcgccgt cgttcacgat gcgcgcgacg tatgcgccga
     3301 tctggttggc gatctcatcg ggcacctgcg gggcgaactc gagcagcggt tcatcgtggg
     3361 gcaccaggaa atcgatgtcg ccgatatgca cgaacgtatc gccgtggacg cgcggcatgt
     3421 gcgcgttcac ctgcgcgatc acgaccgacg cgcactcgag tgcggcgtgg gagatgtcca
     3481 cgctcacccc caggctcaca tagccgttgc tgtcgggggg cgagacctgg atcagcgcga
     3541 cgttgatggg aatgctccgt cggcgcatga gcgccgggac ctgggacagg aagatggggg
     3601 tgtaatcggc caggccgcgg ttcaccgaat cgcgggtgtt gttccccacg aagaaggagt
     3661 tgtgccggaa gttgtaggtg aagcgctcgt cggtgtacgg cgcgaccccc agcgtccata
     3721 cgtggaacac ctcggcctcg gcgaaggcct tggggtggga ctccacgtag gccgtgagcg
     3781 ccgtgaccag gtattgcggc tccccgcagc cggtgctgat gaagatgcgg tcgccgcgcc
     3841 gtatatgcgc aaagacctca tcctccggct tgaacttgtc cggataggcc tccttccatt
     3901 ttaccagatc ctcacgcatg ctcgccgtcc gtgccccgta ttgtaggatt cacccgtccg
     3961 ttcatatcgg gatcacacgg tgccgcgatt gtcaacggca ttattcgata ggtcccgccc
     4021 gtgcgggatc atatcgggat tgattttttt cagggacgat gacacccttc gcgcatgcga
     4081 aacgggaagg ccggcgagga acccatcatc acacgctgcg attcggaaat cgcggacccg
     4141 gaggtgcgcg cgctcctcga tgatgagcgc tatgcctgcc ttcgccgcta cgtcgtcaca
     4201 aacggcttcc ggcggtttta cgtctactat gcgagcggaa cgtcgcgcgc ggcgctcgtc
     4261 gcgccggagg cggtcgtcat cgacgcgcag gaacgatacg accgcgtgac ctacgtcatc
     4321 caggacgatc cctggtcgct cacggacggg cgcctcggcg tccgcgtgtc ccacgacggc
     4381 ggcgagaccg agacgtactc ggcggacaat atcaatcgct tgctcgtgca gggcgagcgg
     4441 atggcgttcg tgaacgattt cgagggtatc gtgaccctcg agggcctcgc cgaggtgtcc
     4501 cgggccgata acatcgcggc gcgcagcctc tttgccgagc tggtgaaggc cgaatactgc
     4561 gcaaagaacg gcgcgcttac ctcgcggcag cggcgggaca tcgaggaaaa ggggctgatc
     4621 cgcttcaact ggaagcgctt cggcctcatc gacgccggcg ccgtcgcgca cgcacaggcg
     4681 cgactccagg gcgcgatcat gggctgtctg cgcgcggaca acccgatcgc cgcttgctac
     4741 accacgcccg cggggctctt caattacgac gattccctga tccgcttcga gccgcatctc
     4801 gggcgcgggg agcataccgt gtactttaat tttctggtgg gcaaccgctt tgggtcgttt
     4861 tcaccgtatt cattcatcga cgtgatgggc gatatcgagt catccaagcg cgtgtatgcc
     4921 gggctcatcg aggcgggcgt catcgaccgg cgggggacgc tcgccgaacc gggagccgtc
     4981 gacgacgcgg ggttcgattt catcgccgat gcggaccggc gcgcgggcgt gcgcgacatc
     5041 gttcgcgcgg cgcggccgct caccctcgac gatgcgcggc gggacattgc cgagagtcgc
     5101 gagcgggtga ccgccgatct ccggcgcctc cgggagcttt ccagcgcata cgagtacacc
     5161 gtcgcgtcgt tttcgcgctt cgtggaccgg ctcgcggggc agcgggcgcg cgcctacctt
     5221 gagcgctatc agggctttcg tatcgtgcag gaggtggccg acgagatcgt gaagaaccgc
     5281 gggatcgtgc tgcgggacgg cgccggcgac gcgctcctcc tgctcaccga cagcgtggag
     5341 cgctcgtcgc cgcactatgc gcggctcctc gcatttttcc gcgggcgctt acccgacgcg
     5401 aagcgcatca gcatcgcctt tgcctatacg aacccgcccg cgcgcgtcaa gcgcgacatg
     5461 ctgcaccccg cggtgcaggc ccttcacgtc atggcgcagg aggaggaatg agggagcgtc
     5521 aacactcgac gcggttgcgg ccgttctgct tggcgaggta gagcttccgg tcggcctcgg
     5581 cgatgagggc gctcgcgtcg agcggccggt catgggtggt cacggcgatc ccgaagctcg
     5641 cggtgagcga cacgggaccg gcggcggtcg cgacggacgc gcgctcgatc ttctcgcgga
     5701 gccgccggat cacctcggcc gcgtgcggca ggtcggtgtc gggaaggatc agcatgaatt
     5761 cctcgccccc gtagcgcgcg accacgtcgc acttgcgcag cccctcagag agcagcgccg
     5821 ccgtcgcctg cagcacctcg tcgcccacgg ggtggccgtg cgtgtcgttg atccgcttga
     5881 agtggtcgat gtccatcatc gccagcgcga gcggctgccg gttgcggggg atacgctcca
     5941 ggtcgcgctc cagcatgaga tggccctgct tgcggttgaa gagcttggtg agacaatcgt
     6001 gcgtggcctt ggtgtagagc tcgcggacta aaaacacaat ctcgagggcg acattgagct
     6061 gcgccgagta ctggctgatg aactccttct cgcccgactc gtagtcgtga tcggtgatga
     6121 catggaaggt gcaccggtag ttccggttgg tctcgagcag cgggatcacg aagagcgtgc
     6181 gcattcgcat ctcggtgagc atccgcaccg tgcgctcgcg gtcgtgccgg aagtagatgc
     6241 tggcgaagct ctgcaggatg tcgccgtggt agatgccgta atccaggatg gcgttgttct
     6301 cggcgtagat gcgtttgatg tcggccagca ccgattccgg ggtgaagcgg gacccgtcgc
     6361 gcgacagccg ctgctcggtc tccgccgaca ccgtgaaggc ggtgtagatg aattcattga
     6421 aggtcttgtc cagcacgctc aggaccacga tgagcgcgcc gaagcgctcg cggatgtggt
     6481 cgcagatgcc ctggatggag tcggaccagt cgccgtcgac catctgcagg agctgataga
     6541 agagccccag gtcggcgttc atgtccaacg gagcgctcgc gccgtccgga ccggtcgaaa
     6601 cggaagtgtc gtcgcctgag cgcatgtgcg tccctacagc gtcgcggcgg cgtcggcgac
     6661 ctcgtcgccc accgcgctcg tcgatttgcc catcttcccg cttgcgacat cgtgaatgga
     6721 gcgtacaacc ttcatcacgg cgcgctcgat gatcgcgccc gccgcctcct cgccaaggaa
     6781 ctcgagcatc atcttcgccg acaggatggc cgcgagcgga ttggcgaggt tctttccggc
     6841 gatgtccggc gcggaaccgt gcactggctc gaacatcgag ggcgccgtct tttcggggtt
     6901 gatattgccg ctggccgcca tgcccatgcc gccctgcgtc acggccccca ggtcggtgat
     6961 gatgtcgccg aacatgttgc cggtcacgat cacgtcgaaa cgctccggct ggttgatcat
     7021 gtgcatgcag gtcgcgtcca cgtggtagta ttcgcgcgcg atgccgggaa agtccttcgc
     7081 gccgatctcg tggaaggcgc gctcccagag gtcgaacacg taggtgagca cgttcgtttt
     7141 cccgcacagc gcgagcgtgt tgcgcttgtt gcgcgcctgt gtaagcgcga aggcccaacg
     7201 caggcagcgc tccacctggt ggcgcgagta gaccatctcc tgtatcgcca cctcgtgcgg
     7261 cgtccccttc atggaaatgc cgccggcgcc ggtgtacacg tcgcccgagt tctcgcgcac
     7321 gaccacgtaa tcgatctctt tcgggccctt gcccacgatg gggcacggga cgttctcata
     7381 gagcttcacc gggcgcaggt tgatgtagag gtcgaggtcg aaccgcagcc ggaggaggat
     7441 gcccttctcc aacacgccgg gacggacgtc cgggtgcccg atcgcgccca gatagatggc
     7501 gtcgaagcgc cgcagctcgt cgatgacgga atcgggcagc acctcgccgg tgcgcaggta
     7561 gcgctctccg cccagatcga attcggcgag gtcgagcgag aatttctccc gcttcgaaac
     7621 ggcgtcgagc accttgagtc cctcgcggac cacctcgggc ccgatgccgt cgccccgcag
     7681 caccgcaatc ttgtatgttt tcatcggtat gattttcctc ttgcaaaatt ttctttacca
     7741 ggtcaccgtt tacgaacagg taaaaatcaa gcaaaaaagg catacccaat gaaaaaatcg
     7801 ctcacgctgc tctttctgct gatctttagc gcgcaacttg tcgccattgc cgacgccggg
     7861 atgtcggtat cagcaaaaaa gaagaactcg tcgaaacatt ccttggtgaa gaagcagtcg
     7921 aaaaaaggca aaggaaccat gtcccgcggg tggaagaaga cgaaaaagcg cggtcggtaa
     7981 cggcaacgcc gtcgcaaacg cgctccctct gagaaaggat tgacaagttt tgttcggacg
     8041 cccgaatgga ccccacaatt cacaagcagg acagtatcga aaaaatattc agatgtcaga
     8101 ataacgcgcc acatggttgg cgcggggagg acatatacaa tggtagcaac aattccaaac
     8161 accgacacca tacggcgcgg gatgcgcagg atcgtcgtct gtatccttgt cgcgttcatc
     8221 gcgttctcgg caacctgttc gtcgcgcgat gtgaagcgaa gcgcccagga agagcagtac
     8281 tttaccgacc tttccaattt cctggcgggc attccggttc cgcaacagag ccgtttctgg
     8341 gaggccaccc agcacaagtt ctacgccgaa cacatcaagt tcatgaacaa cttctgggat
     8401 cgggtcatga acgacaccgt gaatcgcatg gtcccctggc gcgaggcgca catggtccgc
     8461 ggcttcgaga aataccccgc gttctacccg ctcagcggcg ccgactttat caacttctat
     8521 acgatcattc ccgacgcgcc ggagtatgtg atggtcgcca tggagcatgc gggtcccgcg
     8581 cccgagccgc tcaagctcaa tccgcagcag ctccgatcgg gactcgccgc gctcgagcgc
     8641 gtggtgttca catacggcta tttcaactac ttcgcgagcc gcaccatgca gaaggagatg
     8701 agcaatatca gcatcaccgg cgcgacgccg gtcatcatga ttttcatggc ccgcctgggc
     8761 ctgcgggtca cctttgtgga gcccgtcggg atcaacaatg acggcgccct cgagggtatc
     8821 aatggcgcgg gcgccttcgc aaacggcgtc aagcccgagt accgcggcat ccggatcgag
     8881 ttccgcgcgc cgaaggatac gaagacgcgg cagttggtgt acatcagcat gaagatgatg
     8941 aacgacaatg tgcagccgac gacgccctcc ggccgcttct tcgcgcgcta tcagaacaca
     9001 cggacgatga tgaagtcggc cgtctacctc tgccactacg acaagtacaa gggcgtgaag
     9061 gagttcatcc tcggcaagag catcatgctg atccaggacg actccgggat tcccttccgt
     9121 tatttcaaca atgcgcagtg ggacatcaag ctcttcggcg tctacaagcc ctactttaag
     9181 cttgagggct gccatgtcta tcagcagccg gagctgttcg agtcgtacaa gaacaactcg
     9241 agcttccccc tgcccttcca tttcgggtat ggcagcgtga tgggacgcga gcgatcgacg
     9301 ctcatcctgg cggtgcgcaa gcaggacaaa cgggcctagc cgccatcgtc gcgacgcccg
     9361 tacgtgtccg ggcgcgcgaa accgtatgac agccgcatcg gtaaagaaac gactcgtctc
     9421 ctccgtccgg cggacgctgt cgctcggcga tcgcctggcc agcacgggaa aaaagaccta
     9481 cgccctcctg gtgagcctct tcgccatggt cttcctgtat cgcgtcgcgc tcacgatcag
     9541 cctgttcgtg aacgacgtga agcccttcga tttcaacccg aagctcgttc ccgtctggtt
     9601 catgatcggc tttctcccgt acgatctgct gctcatcggc gttatcgtcc ttctctcgtt
     9661 cgccatgagc cgcctttcgc gcgcggaggc gctcgccgcc cgaccgcggc tctactatgg
     9721 cgtgcgcatc ggcgggatcg tgctgaccca ggcgctgttg gtcgtgttgc tcacggtgca
     9781 tgcgacccat gtgcgcctgc tgttcgaggt gcagaccgga ttcgagtact atgtcgtccg
     9841 cgagctcttt ttcaccgcgt cgatcgcggg cgtgctgaaa caggtccctg tcgcatatta
     9901 tctcgccgcg cttgtgttta tcggggtgtt ttggctgatc gcgctcctgc cccacgaagc
     9961 gaaggtccgt ctcgggcgcg ttctcctgat agtcatggcg gtggggctcc tcctctcgcc
    10021 gctctcggcg acactcgtcc ccacggcgct ggtcaaggag atccgcctca atccggtgct
    10081 ctttctgctg tccgatatcg ccgccggcaa ggtctcttcg tcggcgcatc acctggccgt
    10141 cgacgacgcc gaccgcaagg gcatgcagct ggtctcgccc gagtacatca acgagcccgc
    10201 cgagaaagag accaagatca ttcccttgaa aagcaagaag tggaatgttg tttatttcat
    10261 tctcgaatcg gtggggacgc gctacgtgtt cgacacgaag ggcggtccgg cccccatgcc
    10321 gtttttccag ggcctcatga aagagggctg gtacctgaag aaccattacg cggcgtcgaa
    10381 catcagctcg cgcgcgacct tttcgctctt gagcggcctg tacgattact tcgacgaacg
    10441 ggtgttctgc atcgaaccgc acaccacggt cccgtcgatc tatagtttcg tgaaggacag
    10501 ccacgaatcg ttcctggtca cgccggcatc gaccctgtgg tatttccccg actccttcct
    10561 cagaaacagc ggtattcgcg agatccatca tctcgacaat ctcacgctgc ggctgaccga
    10621 ccagaagcac gagaaggccg ggcattacat ggcgcgcgac gagatccaga cggtcgattt
    10681 cttcatcgac cggatcaaga aggcgaaggg gcccttcgtc ggggtgtaca tcagcttcct
    10741 cgcgcatttt ccgtatttcg attacggccc cgcctatcgc gtccggaaca actgcgatca
    10801 gctcatctgc aagtacctga acaatctggc gcttctggat cgcatgatcc agaagctcta
    10861 cgacagcctc aaggagcaga acctgctcga ctcgaccatt ctggtgatcg ccggcgatca
    10921 cgggcaggca ttcgggcaac atgtgcagaa caactacctg cattatcact acagctacac
    10981 cgagaacctg caggcgccgg cgctgttgta tcagccggat attttcaagc cgcgggtgat
    11041 cgagacgccg accagccatg tggacatcgt gcccacgctg ctggacgcca tgcgcgttcc
    11101 tttcgacccg ctcctcctgg cgggcgagtc gttgttcgca aaacacctgc gacggaagta
    11161 catcttcatg tacggccagg agaagacgat ctcgtcgctc tccgccgatc tcatcaaggt
    11221 ctcgttttcg ctgcgtgatt atcgcagctg ggcgttcgac ctgaaatccg atcccgggga
    11281 aaccaggttc ctaccgctcg cgcagtacga gggacagctt gccgccctga aaaaattcgc
    11341 ggggcacacc aatgcaagtc tcaagcagta cagcgccgcc aaccgcgaga agaaggactt
    11401 tcacgggcat cggcatcccc gggtttcgct gtaatccgaa gggatgcggc ctagcgccgc
    11461 agatcgtcgg cgatatcgcg atacaggcgc gccgatccgc tccgggcgat gcccagccgc
    11521 gccgcaccga gcctgccgtc catgtagagg atcgccggaa ggcccagcag ggaatgcatg
    11581 aacaacgatc gatagacggt gctctggaag gtccggatca attttttcag aacatcgtcg
    11641 cgatggcagc ttccccacgg gtcctcgcgg aagatgcgga acaaaagctc atcgagcgaa
    11701 acgagcgtcg atttcacccc caggcgcatg accgggaggt cgcgcaggcc gcgatatcgc
    11761 gtgcgctcgc ggggatattg ttgcgtgacg acacggagga attcccgcgc ccgctcctcg
    11821 cgacgacccg tgaactcgcc gcgcgcaagg cccgcctcga gatggtggcg atgataggcg
    11881 atcgattccg ttaggatcgc gtcccaaaat acccgacgcc gttttgtgta gatatgtccc
    11941 gcgatggcca ggacgagtgc gattgcgccc agtgcggccg gtatgccgat catcgccatg
    12001 tcagccatct agcagcacga acgatacttg cgcaaatagc cgatgagctc gtccagcacg
    12061 tcgtttttcg caaacgagta gtagatgcgg tttccctttt tttcgtattc caggatgccg
    12121 cattccttga gcaccttgag gtgcttggag atgtacggct ggggcagctc gatctcgctc
    12181 atgatgtcgc tcacgcagca tcgccatgtg gggtttttcc ggtgattgtc caccagcgac
    12241 tcaaggattt gcagccggat ggggctggtg agcgcgccga agagggccgc gagacgatcg
    12301 tagggagtgg ggtcgaccgc catcagcagc accctgggat cttcgtggtg tcggttccgc
    12361 agccgcagcc ggtccccttc gggaccgtct tgaggatgtc ctcgcggatg atcgccacca
    12421 gacaaccggt ccccttggtg agctcgatcg cgccggcgtc gcagttgagc cggcaggcgc
    12481 cgcactccat gcaggagcgc acgtcggtga tcgcggcaac gccgtcccgc atgacgatca
    12541 ctccctgcgg acagaccttc acgcactgtc cgcacccgac gcacttcgag gcatcgaacg
    12601 caatcatgac atgactcctt tgacgataat gacgacgagc gcggcgacac ccagcgcgat
    12661 cgtcggtatc aggaaccgga cgatctcccg tttgaccagc gaataattgc tcaccccgct
    12721 gttgccggtg tacgagagcg cgaagaagag gttcgcgccc gccgtgaaga gcgcataaaa
    12781 ggcgctcgtt gatgcgtcgg ggatgccgct cccgttccgg gaggcgagca gcgccatgcc
    12841 cgcgatcatc aaccccgccg tagagaggcc cttgatcgcg aaggtgcggc tcgggagcag
    12901 cgggaacagg accacgtaca ggacaatgct cacggcgatg atgggcagca cctgccagtg
    12961 gatgccggtt gcgaaccagt ggtcccatgc gaagagcccg gcgcaaacga acagaccgaa
    13021 tttcatgaac tgcgcaagcg agggcagcag ggtgaagagc cgatcgcgaa ggtcgaaccg
    13081 gtaggtgtcg gttgcgcggt ccgcgagcgg cgctgtgtca agataggccg ggatgtcgcg
    13141 gcgatacacc gggccgatgc gcggcgagac gccgtttttc cggagctcgc tgagcgacac
    13201 gcccgagagc gagagtttgg gaaggacgag ttcgatccgt tcgccgcgcg tgatgagccg
    13261 ccggtcgtat cgccggagct ggcgcaggat ctcccgcgcg caaaagcgcc ccttgccgct
    13321 ggcgcaccag acattgatgc cgtccgtgtc gatcaccaga atccgcacgg tccgatttcg
    13381 aagcacccgc cacagcaggt ataccgtcag atgataattg ccggtcacca ggaggggggc
    13441 tgccacgtcg tatcgctcgc cgatgaagta caggcccggc tcgatgctga aggtcttccg
    13501 aaacgcgcga aaccattcgt agagcgccac gaatgagtct ttcaccgcgt gaaaaacgag
    13561 gcgtttcggc actccggtga tgaccggaca cgcagctgca atgttcaagg ttttgggttt
    13621 gatggcatcc ccgcagaata tattctcaca atggaatata ctatgcaagg tatattctgt
    13681 caagagaatt tcatgttatt atacagcaaa aagcagccgg gcccgttgct ctatcctgcc
    13741 ctcagatggg cggtcgggac gatagagctc gcggaagagc gcctcaaccg ccgccggacc
    13801 gtcgcgtcgg gcgtcatcgt cccggcccca tccggcgagg atacgacgcg ccgtcgcggg
    13861 tgacgccttg gtcgatttat acatcacgcg ccgcccgtcg cccatcgtta caaacggcgc
    13921 cgagctcagc gacacctcgg tgacgtgcgt ttcgaggttt cgggccgccc gcgcccggcg
    13981 ggtctcgcga tgatgctcgc tatgcgatgg gataaaaaaa taccgcagcg cgccggccgg
    14041 gatgccgccg acatgcaccg gcacgccata gtcgacgccg ttgaggttcg agccgagaag
    14101 ctgggtgccg gcgaccgcca tcacatcgtc gtaggcgatg cgccggtcga aaaggtccac
    14161 ccaaaaggcc gggatgcgcc ggtagcgctc gtacagcagc gaggatgatc ccctccgggc
    14221 gcgctcgcgg tcggtttccg ccgaggccca ggcgaggacg ccgcggagcc agtcgtactg
    14281 ggcgcggtgc ggtaggcccg ccgcctgcgc gtagccgctg gtgcgcacaa atccaagccc
    14341 gcggtagccg tcgtggatcc cgcccggcgc gagctgcgcc acgcaatcgc tcgcccagtt
    14401 gaggcccgtc tcgaacgccg cgagcgcgag ctcgacggag gaaaccggga cggcggcgcc
    14461 gatttcgcgc gcgatgcgcg atcggagcgc gaccaccgcg ctgtcggtga cgtcgaggta
    14521 cccgccgacc ggaaagagcg attcctcgga aggggcgagc gcgcgctccc atcgctcccg
    14581 ggcgagcacg ccgcgtcgta cgggcacggg cgggcggtcg gcggtttcgc gggatcgacc
    14641 ggcacgcgcg aggagcgaat catcccgaag gacgatgtga ttgtgcatga gctggacgag
    14701 cagggccagc ggagcgccgt cgatgatgtg gtcgtaggcc accgcgccga tgcggtacga
    14761 ccgcgcggcc gcgtcgtgga tgatgtcgat gagcaccggc ttggaatact cgcgtccgtc
    14821 ggcgaggtcg accgggtagc cggcgatcag gtccccggcg gggggaagcg cgcccgcccg
    14881 aacgtgcacg tcggcgtcgg ccagcatggc tccgtagcga ccacgggcga gttggagggc
    14941 ctcacgcgca caagacgcca tatcgcaaac gtcgccgcac ggggggagcg cgccgcgcac
    15001 ctcaaggccg aatccgaaat gcttggcgta tttctttcgg aggaagtccc agccggtcgc
    15061 cgggcggtcg gtgttgagcg cgcgaaggag gcggtcgcgc ggatagccca cgatgccgcc
    15121 gaagtggatc aggtgcggta cgatgctcat gtgaggcccc ctcgaatcgt cacgatggca
    15181 tcaacatacg cttttttggg agcggcgtca cggcgaaacg atacaatgta tcggaatgcg
    15241 atgcgcaaaa tacgggcgcg gtaattgcgg ctgtgcggcg gggccagtta cgcgcgtttc
    15301 tggtactcgg tgggggtcgt gccggtcacc cgcttgaagg cccggttgaa ggtcgacagg
    15361 ctctcgaagc ccacggagag ggcgatgtcg atcacgcggg cgtcgcgggt cgcaagccgc
    15421 cgtatcgcct cctcgatgcg cagccggttc acgtactcgc cgatcttctc gccggtgaac
    15481 gagacgaaga ggcggcccag gtagctggga ttgaggtcca ccgcggcggc cagcccctcg
    15541 cgcgagaggt ccgacgcgta gtgctcgcgg atgaagtcga tcaccctgcg cagcttttcc
    15601 tcggcgcttt cggtgagcgc ggggcggtcc ggtgaagcgg gatcggaggc gcccgcgggg
    15661 ggccgccccg ggcgctggag ctggtcgtac aggcgcgcgt gctccacctt gatgcgcgca
    15721 tagtcgccga taaacagcgc catgaacagc accacgatga tcggcgagcc gtaggtggcg
    15781 atcatcccgt ggccgggatg tcccgccagc gcaatggcgt tttccaccgc gagcatggcg
    15841 atggcgagcg agagcaaggg ggccaggatt cgaatgcgcg tcgtatcgcg gtgcagcgcg
    15901 tgcaaccgga tcagcagtat gcagaagtaa cccgcgccaa ggccgatgca caggaaattg
    15961 atgaggtacg atccgctgcg ccccacaagc cccggaacgg catatgtgtt gatgagctgc
    16021 gccgctgcga gcaaagcgag cgccggcagc gcgatccggt ttacggtcgt cagataacga
    16081 ccgtaggcgg cctggacgat gaaggccagg accacgatga aaaacggaac cgacagcaat
    16141 acgaaactca tggcgcccat gagccgttgg tcggtggtgc agtcgatcac gcgcggcgga
    16201 tagggaaaaa agagcgccag gatatacgcc gcgtaatacg ccagtcccag cgcgctgtag
    16261 aggagcttcc gctcgcgcga ggcgccgaag aacacgagga gcagcacgat ggtcccgcag
    16321 tacaggaaca cgatgccgaa ggggatcatg gtgagccaga aatgctgttg gcgttcccgc
    16381 ttcgcgaacg cctcgccgtc aagaatgcgc gcctcggcgg cgaacccgcc gtattcgcgc
    16441 ccgaagatgc cgagacgaac ggagacctcg tttcgccccg gcgcgatcac gccgggcgca
    16501 atggcatagt tgcgcgggga gtgcaggcgg aagaggtccg tccccgtgcg tcggccgatc
    16561 ggctgcgcat tgatgaagac ctcgtcggcg tggtacacgc gaccgaggga gatgccgtac
    16621 cagcgtcccg gatcggcccc ggcgtcgaat gagccgcgaa gccatacatg gcccatcgta
    16681 tcgaagcggt ttccggggat ggtgaaggtc gcgggcatgg agatgggacg ccacccggtg
    16741 gccgatgcgg cctgcgcatg ggtgcgggac ccgtcgttga ggatctccca gcgctccacg
    16801 ggcgccccat cggtcccgtc gcccccgcgg caggaagcga gcgcaatgca catgagcatc
    16861 gtcagggcga acagcgttat cgttcgccga gtttcagtca cgcgcgcccc gttcctcaag
    16921 cagtcgaata aacgcatcgc ataattcgcg ctgggttcgg tatttccgga cctggctctt
    16981 caagtattca tcgcttccct gcttgattgt ccgcagccgc gcggtatatt cgcgctcgcg
    17041 agcggcgtgc tccccgcttc gctttttggc gcgtgaaagg gccgtgtggc cttccttgtt
    17101 cgcccggttc gggtccgcgc gcgcgtcgag caggagcttc gccgcatcgc agtcgcagag
    17161 gctggcggcc tcgatgagcg gggtgtcccc ccaccggtcc gtttcgtcga tgcgcagccc
    17221 ggcggcgatg agcataccaa ggagctcgtc gctgccggtc gacacggcgt gatggagcgc
    17281 cgttctgccg tccttgtcgg cgatatgtac gtttaccagc ggctgcgcaa gagcgcgctt
    17341 gaccatcgcc acatccgtgc gccgggtgta gtggatgata ttgtaactgc tgaaaaagcg
    17401 ctcgtagacg gcccacgaac cggtgaccgg cagaagaagc gtgtccagga ccaggctgaa
    17461 cggaaggtcg atgatcacca cggtcatggt cggccagccg ccgacgggat tgtgggggtg
    17521 cggcggcgtg gtgaggatcc ggaagtccgt ccgggttccg ctgtagacga gcggttcgcc
    17581 gaagtcgcgc gtcgcaagag acgcgatcgt tacgcaccca agcgacgcca tgcccgcaca
    17641 gcccaaaaga agaattaata tgatggcgcg tcggcgcatg atccatcccc ctcatgggtc
    17701 tcggtccggt atctgatcag cgtacaggaa aaaccgcaat cactaatttg acgacgacgc
    17761 tcaagcgggc gcatcattga aaaaaagatt gaattttccc tgcgcaattc gccttgtcgc
    17821 gtatcgtgaa aaacttgaat atcattcgat tgatgctatt ctgatagcga aaaagcgggc
    17881 atgtccgaca acacgaaaca acagaggatc accttccggc gcggcatcgt cgcgacgatc
    17941 atcgggatcg cgcacgtcgt ggtcggatgc gccgggacga tgtgggcgat cgcgcgcatc
    18001 gcggtcatcg gctaccgggc gccgctcgag atgggcgcgt ggatggccgc cttcaactat
    18061 ctgtacgagg ggcatgtggc gctgctctgg ctctcgggaa tcggccttct gcgcggacgg
    18121 gcgtgggggc gcgccctggc gctcgtctgg gccgcgctct cgatcgttct gcatgtcgcg
    18181 gcgtacggga tccagcgcca ctactggggg ctcctttcgc ccggcttcgg ctggggcgat
    18241 tatttcgttc tgtactacgc cggcgcgctt gtcgttgcga tcgccggttc atgggcgctg
    18301 ggaaggctcc ccggctgggc gcgcgcgctg tccgaactgg tcggacgcga cacccccttc
    18361 gcgcgcgtac tcgaggcgct tgcgcgccca acggagcgct ccaagccatg acccccatcg
    18421 gacacatggc caccggcgtc gccgcgggcg ggatcatcgc cccggtcatc aacaaggcct
    18481 tcaagacgcc ctatcgcgcg ctcattccca tctatctggt cgcctccgtg atccccgacg
    18541 tggacggtct ttcgctcctg gtgagccgcg cggcgtattt cgggaaggcc tggtactcgc
    18601 accacatggc ggggcactcg atcatcggcg cggccgtgta tgcgacggtg ctggcgctct
    18661 tctatctcct gttcgccgta acggggcgcg gcgtcatcaa cctgtttcga atggacaagg
    18721 tgccgttgga gcaccggctt cgccgcaccg tcggcgcgtg gatcgccgcc ttcatcggct
    18781 gcctggccca ttacgcgggc gaccttccca cgccgcccgg cccctggggc gggatcgccc
    18841 tcatgtggcc cagcatgacg atgtacggcg gctggaaccg catcttctgg cataactggt
    18901 atctcatcta cttgtcgatc gggttcctcg ggtcgtacct ggccgtgcag ctggtcgccg
    18961 ggttcttcgc cgccttcacg ccgcgggtga tgcgctggac gggatatctg ttccggaccg
    19021 cggccgtggt cttgtgcgcg ctgtttctct atcagacggc ggcgttcgtt cacaagcacg
    19081 atttgcgaac aatggggccg gcgcgctggg acgcgctcaa ccgctcgctg gtgcccgcga
    19141 aatacatgaa gcgcgccgac gcctactacg acaaagcgac ggtcttctgg cgcagaagcg
    19201 tcatcaccat gcccgatatc gtcaagtacg ggaacatcac cctgcatttc ttcgagaacc
    19261 tgcaccggcg cgtcgcgccg gcggtggggc gcttcatgcc ggtcacgcgg acgcgggagg
    19321 acgatatggc gctctatcgc gcgctccagg cgatggcgcc gggtatggaa gacaaccgtc
    19381 cgggcgacta ccgcgtctgg atcctgcgcg atgcgctccc gcgtccggac tatttcaacc
    19441 gcgggttcct gtaccagctc attcaccgga cgaagaagca catcatgcag atgaccaatg
    19501 cctggatggt ggtgatccac atcgaggagc gggacggcgc gggcaacgcg gtccgcgtga
    19561 agaagatcta tcacaatgac aaaatcttcg tgtccgatcg catcatgccg cagctcaatc
    19621 tgcacgagct tccgcaggtc acctttaaat tctgggagca cgatcgggtc cagtactcgc
    19681 tgatccccgg ctccgacctc acgcgctttc acaacctcgc gaagggctac tacctcacga
    19741 tatcgacggt gcgcgggcac ctgtggccga actggcgctc cggcgtgctc atccacgacg
    19801 ggccctggac ggagggatgc atcatccacg cctacctgca cacggacatc ggccgccatc
    19861 tcgagtgggc ctactacccg ctctgggagc gcgccgacga gatcatcaag aaggacatcc
    19921 gcggcaccgt ctatcgcggc aggaacatct actgggggcg gttgctgttc atcaaggacc
    19981 ccgttcgcct cgagacgctc gcgcaacggg ctccggtaac tccgcaggtg gcgcattccc
    20041 aataacgttt ccgcgacggg ccatgttttt ttgtttacgt ctcgccggtc ccgggatatc
    20101 gggtgcacca cttcctacaa tcggagatgc gtcatgaatc gtgttcgcac tgcctgttgc
    20161 attgttggca tgctcgtggt gtgcgccgtc ccggtcaggc cggacgatgc gccaaacctg
    20221 cggcccctcg tcgagggcaa tacccagttt gcgctcgcgc tatacaagag cctcgtcgtc
    20281 cccggaaaaa acctggtcgt ttcgccctac agcgtatcga cgtcccttgc gatgacctcg
    20341 atgggcgcgt gggggaagac gctgcaggaa atgcaccgcg ccctgcggct gtcgatgaat
    20401 tccgaggcga tccacgggtc cttcgcagtc ctcgccgacg cgctggaaca gcgttcgggc
    20461 gcgggggtgc ggctcactat cgccaacggc atctggctca acaaggggct ggaggtaaaa
    20521 aaacagttcc tgacgtcggc ggaacggagc tacaaggcgc gcgccgacca gcttgatttt
    20581 tcgggcaacg ccgaatcgtc acggcgggag atcaatgagt ggatttccaa gaaaaccgcg
    20641 ggaaggattg cggggcttct tccccccggc tccgtgagcc ccgcaacgag tctggtgctc
    20701 gccaatgcgc tgtatttcct ggggaattgg gaatacccgt tcgatccgaa gaagacgcat
    20761 accgggaagt tcattattgg gaagaaacgg caggtcccgg cggtcatgat gcgtggaaag
    20821 atgctctgcc gatttctgga aacctcccag gcgcacattg cgagccttcc ctataagggc
    20881 ggtgccctct cgcttgtggt gtttcttccc tcggccaagc tggggatcga aaaattcgaa
    20941 aagggactta cggcgcagat gatgtcccaa tggctgtcgc gtcttcagcg gcgcgagatg
    21001 gagatcacga tcccccgctt caccatcgcc gcatcgttgc ggctgaacga gacgcttaaa
    21061 aagatgggca tgtcctccgc attcggtccc gcggccgatt tttccggaat cggcggaaag
    21121 ctgttcatct cggacgtgtt ccactcggcc tacatcgagg tcaacgaaac ggggacggag
    21181 gccgccgcct cgacagctgt atcgatgacc aagaacggct cgcggcgcgc cttccgcgcc
    21241 gaccgcccct tcgtgtacgc tatcgtcgac aatgcgacgg gaagcgtcct gttcatgggg
    21301 cgggtatatc aaccgaaggg ataatggagg tcatatgctc gacgtcaaat cgctgcgcta
    21361 tcataccgag aaggattatc ttgtgctatc catcgtcttt ggcatgctat actggattct
    21421 tctgatcgtg cttaccttcg ggatcatcat ccccattgtg gccctcgtcg ccctgggcct
    21481 gtggatcttc gagcagtttc tcaaggcgat gctcttcggc gacgccatcc gcgtttcgga
    21541 aaatcagtat ccggaactca acgccatcgt ggtcgatcag tgccgggagt tggggatgtc
    21601 gaacgcgccg tcggtgttca tcgtcaacgg ccaggggatg attaacgcca tggccgtacg
    21661 ggtcctgtcc aagcgctatg tgatccttct ctccgacctg gtggacctca tgctcgcgcg
    21721 cgatcagata cgggagctgg cgatgatcat cgggcacgag ctcgcccatc acgccgcggg
    21781 gcatctcgat cccaagcgga acctcgcgat cgcgccggcc cgcttcattc cgtttctcgg
    21841 gaccgcgtac agccgtgcct gcgaatacac ctgcgatcgc atcggctatg cgttgatccg
    21901 cgacaaggcg gcggcgcagc gggcgctcat cgccgtggcc ctcggcgcgc gcaccctcgc
    21961 ctcgaagacg aacattgacg cctttgccgc gcaggagcgc gaggtgccgc gctttttcgg
    22021 ggtgctcacc ctcattttct ccacgcaccc ccgcatgacg ctgcggatca gacaactcga
    22081 ggggttcgag cccaaataat cattgacagg cgcggcgcag gggcgacaat cgggtgaatt
    22141 atgtcacgcg actggtcgcg tcatcaaacc atactaatcc atcagaggta tccaatgaaa
    22201 cgaatatgta tcgtagcgct cgtcgccatg gtcgcgcttg tcgccgtttc gtgcgccaag
    22261 aaaaaaccca gcgacatctt caccacgctc cagaagctcg gtgacaccaa ggagatcgcc
    22321 gatctcaagc aggtgtacac cgccgaaacc gtgaaggcga tggaagagct catgaagatc
    22381 gtccccaagg acctgcagga cagcaagggg cagaacaagt tcccgaagga agcgaaatgg
    22441 gacgtcgtga gcgagaagat cgacggcgat accgccgagg tcaaggtcaa gtttagcgac
    22501 caccccatgg cgaacatgaa gggccaggaa gtgaccttca agatgaagaa ggaagatgga
    22561 gcctggaaaa tcgatcttga aaaggaaatg aaagctgccc tggcgctctt tcaggggctc
    22621 ggcggaaagg acgggctcct caaggacctc aagaaattta ttaagtagta tcggttatcg
    22681 tgcaagaccg tgttgcgttc agggtcaggt gaatcacctg accctgaact tttttatttg
    22741 acagatattc gattctattt acgtatgcat gcggaaggtt gtccgagtgg tcgatggagg
    22801 cggtcttgaa aaccgttggg tcacaagccc cgggggttcg aatccctcac cttccgattg
    22861 aagcagcctg gagagattcc cgagaggccg aaggggatgg tttgctaaac cattgtactg
    22921 tgaaaggtac cgagggttcg aatccctctc tctccgaagt tggtgcggga ccgaacgatg
    22981 ccattgaaaa agctcacaca gcgaattgac gggaagcggg acgccctgaa gcggacggtc
    23041 gcggcgcggc gcgcgcagtt gcaggcgcgc atcggcgaag cccgcgcttt tgcggccggc
    23101 cggattgagt ttggcgtccg gaaattccgc gaataccgga ccatcgcgtt cctgccggag
    23161 aacgcccggt ttttcgccgc cgcggcgtat gtgccgtttg tcggctggat gctccccctg
    23221 tacctcaagg aggattcatc cttctgtcag gagtccggaa agcagggatt tgtgcttgcg
    23281 gttttttttg cggcgattcc catggcccta tcgcttctcg gggtcctgtt cgtcccgaag
    23341 gatgcgcgca tcgtaagcct tgtgctggcg attctcgtgt atctttcaaa tagcgtgtac
    23401 ctcgttctgt gcgtcatcag cgcgttcatg gccatcagga cggagaggct gcttgcggtg
    23461 ccggttgcgg cgtcgtatgc gcagcggctt cagttgtaat caggagcgaa tgcacgccga
    23521 cgcggacccg ttccgcccgg cacgtcatat gcgtctatag ctcagctgga tagagcgccg
    23581 gactacgaat ccgtaggtca gaggttcgaa tcctcttaga cgcgcaccgg aggccgcccc
    23641 gtgaggggcg gcctcttcgt ctttaccgga ctgcgtccct ctcccgcgcg atggatccca
    23701 gggcacgaca tcggcgcatg ggcaatatct acttgaaaaa tatccgatat cgtgtcagca
    23761 tcatccggtg aatgacgtac accatgagtt catgcgcgcc gcgctcgagg aggcgcgcaa
    23821 tgccgcagaa gcgggcgaga tccccgtggg ggccgtgatc gtgcgcgccg gcgcggtgat
    23881 cgcgcgcgcc cacaaccgga accgccgcga tcgcaatccc cttctgcacg cggagatgat
    23941 cgcgatcgag atcgcctgcc gagcgatggg caacgagcgg ctgatcgact gcgacctgta
    24001 cgtcaccaag gagccctgcg cgatgtgcgc gggggccctc gtgcacgcgc ggcttcgggc
    24061 cgtctacatc ggcgcgcgcg atgtgaaata cggcgcctgc ggcacggtgc tcgcggtctg
    24121 cgggagcccg gcgctcaatc accgacccgc catcgagttt ggcctgttgg agcaggaatc
    24181 aacggcgctg ctgggcggtt tttttaagcg gctgcgcaat caccacacaa aggagagacc
    24241 ggaatgaacc gtcgattgtc gattgccgtg ctggcgctca tcgcggcgtt gctcgcaagc
    24301 tgcgcgacca tctcgtatca cgacaggatg aaatactacg gcagcccccg cagcgagttc
    24361 gtcgacacga ccgaagcgcg ctcgatgttc gaaggtgttc tcgtcgacga ccgcctgata
    24421 cgaaacggga agccctacta ccatctccag ttcgagcgcg tcatggacgg cgcgggccgg
    24481 tacctggacc tgtatctccc ccatgacacg agcgatgcat cgctcgtctt cgagtccaat
    24541 gtgaaaaaac cggggaaacg cgcgtcgttc cttctgttgg agcggacctg ctgctcgaat
    24601 gagtacggcg agttctccga cccgctcaag acgcgtgacc tcgaaaaaaa cccggagcgt
    24661 ctgttcgaca tcgtcaggga caagctgaaa aaaaacgtca cgcgcgcgga cctgccgatc
    24721 gtctttgtca aactggtctt ctcgaacgtc ttcaaatacg acattatcta caccgtgtgg
    24781 aaggcggagg ggggcgacgc gacgtcgttt tcctggaacg cctgcctgca gctctcctat
    24841 gacaccatcg acgtgaaatg gaaaaatcgg agcagggcgc tgtatggact ttccaaggtg
    24901 ggctatgtgg ggccggtcat cgtcgatatc gtgacctcgc ccatacagct gatcgcggcg
    24961 attctctatt tcgggatggg cggcgcggtg aagtgagtcg gccgcctagg ccttgatgcg
    25021 gaaatcgccg atacgcttga tggcgccgtc ggtctcgatc cgattgagca gggggatgat
    25081 gtactttcgc gacaggcccg tcgcgtcctt cgcctcgggg acggtgagct tgtcgcggcg
    25141 atcgaactcg gcgaggacga gtcgtttcag ttcatcatac acctcccgat ggaagatgat
    25201 gctcccgtcc aggctcacca gaaacccgag cttcacgagc tcccggatcc gccccttggc
    25261 ggcatcgtcc gccttgtcga gctccacgcc ctcgcggccc ttcgcccgga catcgtccag
    25321 gacctttttc cgatcgggcg agagggtgtc cggcgtcacg gcgttcccgg cgtaatagcg
    25381 cccgtccttg tcgacgatac catgctccgc ggcgaccgcc ggcatcagca gccggcagaa
    25441 ttccgggtcg atgccgcagc ggtccgcgat ctccttgatg ttcagtccca cggcccccat
    25501 gaccgcgtcc cggatggccg ccatcgacgc ggcgccgtag gcgggatcga cgagcagggg
    25561 cccctgcgcc gttatgcgct tctcattgat cagcgttgtg ataatctttt caatcgcctt
    25621 gggattgtcc gggagcgaat tgatgacgtc cgcgcgcggc acggcgcccc ggaccgcgac
    25681 gctgaacgcg atgaggtctt cccggccgaa gcccgcaagc gcgccgacct tcgcgcgaag
    25741 ggcgcgcatg ttgcgcaggc cgccgtactc ggtgagcacg acgaaccctc cgccgatgat
    25801 ccggtgcccg ccggggtgcg tgaccacgaa gggctggccg ggatagaaaa accacggctc
    25861 gtcgaacttg atgcgcgcga tgaccgtgcg cgagccgtcg tctggctcgt gcgccaggat
    25921 gagcttggcc tttcgcgtcg aggtgccgat gagcacctcg atcccgatgt tgttcctgat
    25981 gcggcgctcg cccagaatgc gcaggcgcgt caggatgtcg ccggactccg cgaagaagtt
    26041 gtcgcgcgcg acgatggcgc cgcgctcgat ctcctcgcgc gcgatccccg caaggttcag
    26101 cgccgtccgc tgcgagggcg tcccctcggc gagcgcctgg aagtggctct cgatcttttt
    26161 tatgcgcacc gcgcgccgct ggggcagcac cgtcacctcg tcctcctcgt ggaagcggcc
    26221 gttcttgagc gtcccggtca cgatggtgcc gtatcccttc gaggtgaaca cgcggtccac
    26281 gtagaggtac ggccgtccgg cgtccccggc cttggggagt tttcccaggt tctgaaggat
    26341 gagttgtttg agctcgtcga cgcccgcgcc ggtgcgcgcc gagacgcgcg ccaggtccgc
    26401 gtccgcgtag cgggtgcccg cgaggtgctc tgcgatgtcg gccgtgacga gctcgatggt
    26461 ctcgtcgtcg accatgtcga ttttattgat gacggcgatg atgcgctcga tgcccaacag
    26521 ctccagcacg cggaagtggt cctcggtctg gggcatccaa ccgtcgtcgg ccgcgaccac
    26581 caacagcgcc aggtcgatcc cccaggcgcc ggccaccatg ttccggatga agcgctcgtg
    26641 cccgggcacg tcgatgacgc tcaccgtgcc gaatttcgga tactcgatgt tggcgaagcc
    26701 gatgtcgatg gtcatctccc gctgtttttc ctcggggagg cggtcgcagt cgatgccggt
    26761 gagcgcgcgg atcagcgagg tcttgccgtg gtcgatgtgc cccgaggtcc cgatgatgaa
    26821 catctaggcc cccgagaaga gcgccgtgag cgcctgcacg atgacgggga tgtcctcctc
    26881 cagaacggtg cggaaatcga gcgtgtattt tccgtcggcg atgaagccga cgatgggcac
    26941 ctcgttcgcc gtgagcgctc ggtcgatatc gtcggcgcgc aggccgggga cgtcgatctg
    27001 cacgccgacg ctttgcagcg tgtagcccgg catcgacccg cccccgaagg tgctcttgca
    27061 ctcgacgcgg gcgaccatct cctttttcct ctcgtgggtg atccgcttca tggtccgctc
    27121 gatctttttg gcgacggccg cgcggtcctg gaagatgccg ctccacacgc ccacctcgcg
    27181 accgcgttcg ttcatgtagg cgagcagggt ttcctgcagg atgaagtagg aaatcttgtc
    27241 gacgcgcaac gcgcgcatca gcgggtgttt ccgcagctgc gcgatgaggt cgcgtcgtcc
    27301 cacgatgatc ccggcctggc agcttccgag gagcttgtcg ccgctgaagc agacgaggtc
    27361 cggcccttgg gcgacctcga agcgcacctc cggctcgaag gagagcggca ggcgctcgtc
    27421 gcggatcagg ttgccgcttc ccaggtcgcg cacgaagagc accgagtcac tggccagcga
    27481 ggcgagctcg cgcagcgtcg gttgccgcac gaagcccttc tggcggaaat tggactggtg
    27541 cacggagagg atcatcgccg tgtcgggggt gatggcccgc ttgaagtcgt ccagttcggt
    27601 gatattggtg gtgcccacct cgaccaggcg cgcgcccgtc tgccgcatga tgtcgggtat
    27661 gcggaagccg ccgccgatct gcacgagctc gccgcgcgag acgatcgcct cgcggccggc
    27721 ggcgaaggcg tgcaggatga ggaacacgct tgcggcattg ttgttcacga tgagcgcgtc
    27781 ctgcgcgccg gtgagatggc aggcgagctc ctcggcgaac ccgccgcgct ttccccgctc
    27841 ccccgtggga agatgcacct cgaggttgca gtaccccgcc atggccgcgg ccagccgctg
    27901 cagtatctcc gcgcggaggg gcgaacggcc caggttcgtg tggatgatga cgccggtgcc
    27961 gttcaccacg cgctgcagct tctccagccg cttgcgcccg caggccccga tgatggaccc
    28021 gcggagctcc tcggccgaac ccgacgcgcc ggattcgacc cgtcgccgga aatcgtcgat
    28081 ggcgtcgcgg atgaccgcca ccgtcacgtc ccggccgatg accgggacga aacgggccac
    28141 atcatcgtcc tggaggatct tttcgacctg cgggatgttt cggaggatgg aggcgatatc
    28201 gtcgctcatg gtggctgttc cccgtcgttc gcaccggacg gccgccggcc cggcgcgcat
    28261 tgttcggaag gggcaggaat cgaacctgcc gctgcactaa agtacagcca acggttttga
    28321 agaccgcaga ggccaccaga cctcatccgc ttcccgaatc gtcgattcac attatgcatt
    28381 ctcggttcga acgattgtgt caatactttt tacccggaag gggggcaaat gatcgatgcg
    28441 agggagcgtc aatcgtcgtg aagaaaatat tgacgcggtg ttgacgaacg agtatcttat
    28501 gaaggcggca tactgtccgg ataccgagat tcgggaggtg ccgcaaaccg cgcgctgata
    28561 tgcggatcga taaagaatgc gcacggtggt agagatcgcc cgtatgcgta cgggggattt
    28621 ttgttccgcc gccggtggtg gcgtgaacgc gggatggaaa tagagtggac gacggggtaa
    28681 acagccagcg gttccggttg ctcttcgagc gcgcccgcga tgcgatgtgc gtcgtcgagg
    28741 acgacgtgat cgtggagtgc aacggggtga tgctcgacct gtttcgctgc gcccgcggcg
    28801 atatcctggg aaaatgtccg gcggacgttt ccccggccct gcaacccgac ggttcgccgt
    28861 cacgtgaacg gatgcgggcg ctcttcgatt gcgcgctcgc cgggccggcc cagcggtacg
    28921 aatggcgaca tgagcgtccc gacggcagtc agttcgacgc cgaggtgagc ctcaccggga
    28981 tcggcttcaa tggaaaccgc cacctacttg cgatgattcg caccgtctcc ccgtgcgccg
    29041 acgcggacca cgctgtccgg gagagcaagc ggaaataccg ggacctggcc gagcagctcc
    29101 cggtgatggt cttcgagatc gaccgcttcg acggcatcgt gttcatcaac cgccacatcc
    29161 tgcacacatt cggcgaccgg ctcttcgacc aggataatcg gctcaactat gtcgagatca
    29221 tcgcggcgga ggatcgcgcg gtgttcgagg gcctgctgcg gcgcgtgaac gggggcgagg
    29281 aggcgcacaa tgtcgtgctg cgcggtctca ccggagcggg aacgcccttt ccgatgcagc
    29341 tctcgctggt gccggtccac gatgacggcg ccatcatcgg cgttcgcggg atcatcatcg
    29401 acatcacgga acaacaggcg cgcgaggacg ccctgcgcgc gagcgaggag cggtatcgcg
    29461 ccatcatcga aaacatacag gacggatatt acgaggtgga cctcaagggc aacttcgtga
    29521 acttcaactc ggccctggcg cacatgctgg gcatcgcgcg tggtgagctc atgggagtga
    29581 actatcgggc ctacacctcg ccggagattg ccgatacgat caatcggagg ttccacgagg
    29641 tgttttcgac cggcacgccg ctggggacgc tcccctggga aatttcccgt gctgacggga
    29701 cgcagcggtt cctcgagatg tccgtttcgc tcatctattc gccctcgggc gatcccgcgg
    29761 ggttccgcgg aatcgtgcgg gacgcgaccg atcggacgcg cctgctggag ctcatccgcg
    29821 ccagcgagga gcgctaccgc gccatcatca agaacatcga ggacgggtac tacgagctcg
    29881 acctcagggg caacgtgacg tttttcaacg agtccttccg gcggatcatg gagtatccgc
    29941 acgaggagct cctgggactc aattaccggc gctataccga cgagcacacc cagcggcggc
    30001 tccgggaggc gttcaatgcg gtgtatcgca ccggagcgcc caacaagaac atcgagtacg
    30061 acatcgtccg taaaaacggc gagcgccgct cggtggaggt gtcggtgacg ctcatcttca
    30121 atgcggccgg ggagtcgatc gggttcctgg gcatcatgcg ggacagcacc gagcgcaagc
    30181 gggcgcagga ggcgttgcgc gcaagcgagg agcgctatcg cacgatcatc gagaacatcg
    30241 cggacgtcta ctacgagctc gatcttacgg gcaccatcgt gtttatcaat gaaccggggc
    30301 tcgccatgtt caatgcgacc cgcgaggagc tcgtcggatc gcacttcaag aggttttcgg
    30361 acaagaactt cgtccgcacc atgtacgacg cggccgcgcg cgtgctggcc accggggttc
    30421 cgtgcagcgg aatcgactgg gagatcaccc gcacggacgg gtccacgttg acggggaacg
    30481 tctcgatctc gctgatccgc gacgcgaacg gcgagcccgc gggatttcgc gggattctgc
    30541 gggacaccac cgagcgccgt cgcgcggagg acatgatcaa gatgctggcc tatcacgata
    30601 tgctcacggg gctgcccaac cgcgtgctcc tgaacgaccg actggcgatg tcgatgaact
    30661 acgcggtccg ctatcacaag aagctcgcgg tgctgctctt cgacctggac ggcttcaagg
    30721 aggtgaacga cacctacggg caccagacgg gcgacctatt gcttcaaaag gtgagcaatc
    30781 gcctgcttgc gctcatccga aagagcgaca cgctcgcgcg cctgggcggg gacgagttta
    30841 tcgtcctgtt gaacgatatc gcccggaccg acgatgcgct cgccttcgcg gacaagatcg
    30901 tggagtcgtt cagggagccg ttccattgca gcgagctcga catacgatgc aatgccagcg
    30961 tcgggatcgc gctgtaccca gaacacggcg acgacatgga aacgcttttg cagaagtccg
    31021 atgaggcgat gtatcgtgcg aagcgcggcg gcgcgggcaa gtacgaactg tttcattgac
    31081 gcgcccgtcg agcccgggta tgaatgaaaa acagccgaag cgaattgtgt aatcctgttt
    31141 cacggggcgt tatccgcgca cgggttttca ttccggcgcg gtacacatat ctactcatgg
    31201 aggaatgtca tgaaatacgt cagtggtgtc gtcgctctct gtatggtgat cgggcttgcc
    31261 gcctgcgcga agggccccgt catatcgctc gaaaagaacg agctcgcaac gagtgcgacc
    31321 agcctcaagg tcctggtggc caatccggtg aaggggaccg atcagtcgca aaaacgctat
    31381 tgggtgtgcc tggcgaaagc gggaacgccg gattcggagt ggggggactg gtcgttcgtg
    31441 tacgacaata tgaccgccat cgacgtcgcg attaaaaaga acctcgttga cggaaagctc
    31501 cccgccgggg actacgaggt acgcctccat agcgagtatc ccaccaagcc gcacaatgtc
    31561 gtcatgcgca tcaaggtcgc ggtcaagtag cgccgcgcat ctgcacacgc tcgcgcgcat
    31621 tgccaacgtc aacgacggtc ctcgcggccg tcgtttcgtt ttacggctta tggggtgatg
    31681 gcgatcgctg gccgctcgct ctcccgcacg ctgccgatga tccgcgcatc ggggcagccg
    31741 cacgcgtgaa gcgcagcgca taacgcctcg gcctccgctg cggggagcga gaccagcagg
    31801 ccgcccgagg tctgcgggtc gaagacgatg tcggccacct cgcgcgggac cgacggatcg
    31861 acggtgcaga gcgcgccgac atggtcccgg ttccggtaca tgccggccgg gatgagcccg
    31921 ctcgatgcgt actcggcggc gcccggaatc agcgggacgg ccttcgcatc gatgtggact
    31981 gcgaccgggt cgttcccgat catttccttg agatggccca tgaggccaaa gccggtgacg
    32041 tccgtacacg catggacggt gcgcccctcg aggcacgcgg cggcgtcgcg attgagcagg
    32101 gtcatgctgc ggatggcgga tgcggcgtga gccggatccg ccataccggc cttgaccgcc
    32161 gtattcacga ttcccgttcc cagcgccttg gagagaacga tcgcgtcgcc cgggcgaacg
    32221 ccggtgttcg tgagcacccg atcggggtgg acgatgcccg tgaccgagac gccgtacttg
    32281 aactcgggat cgtccacgct gtggccgccc aggagctgga ccccggccgt atcgagcacg
    32341 ctctgaccgc cctgtaggac gcgcgccagg gtgtccaggc tgaaggtgga caccgggaag
    32401 cagacgatgt tgagggccgt gagggggcgg ccgcccatcg catagatgtc ggagaggccg
    32461 ttggtgacgg cgatttgccc gtagacgaac gggtcgtcga cgatgggcgt gatgaaatcg
    32521 agcgtctgaa tgagcgcgat ctcgtcggtg agccggtaca cgccggcatc gtcgctggtg
    32581 tccatcccca cgatgacgtt cgcatcgacg ggcaccacga gttgtttcat gacggccgcg
    32641 aggtcacccg gacctatctt ggccgctcac ccggcgccgc ggacgctctc ggtgagccgg
    32701 accgcgtcca ggggcgcatt gatggaacac tgttctttca tg
//
LOCUS       DAORHF010000008        32737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_756_length_32737_cov_6.305795, whole genome shotgun sequence.
ACCESSION   DAORHF010000008 DAORHF010000000
VERSION     DAORHF010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32737
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_756_length_32737_cov_6.305795"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKJ16_01485"
     CDS             <1..523
                     /locus_tag="PKJ16_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45681.1"
                     /translation="WAWEDGATAIGINVSAARFASYPTMMVFNSKLYALWEEGNISAV
                     QQIRFGVYTGGGVWNKLNTDVNCGINYNCAQNARRPSMAVCWGSLHLAWYETNGSGFN
                     QIQVMTYNGNDASPDGISITGQSNPEYGLNYNPSMNAAYPVLVGLNDNLYVAWYESNG
                     TANQIRVAAASWY"
     gene            579..1217
                     /locus_tag="PKJ16_01490"
     CDS             579..1217
                     /locus_tag="PKJ16_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HNV45682.1"
                     /translation="MVKLDNPYPDSSYRSVLAPVALLFALGFVCCRVSFFSGLYQYAI
                     LLFVVILVACFFDRGLIGRDRLFSWPSSVSHLVVGITAAVALFFAVYILLRLTRNIAA
                     FSIYFTAGGALSKWELFATAAVLLPVIEEALFRGFIQHNLANSFGAHTGLALGSAIAI
                     LFSLLFTRAVWVALPLAALTLLTGFLYMRYRSLAVSIAAHGSFALLLYVFVV"
     gene            complement(1218..2129)
                     /locus_tag="PKJ16_01495"
     CDS             complement(1218..2129)
                     /locus_tag="PKJ16_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV45683.1"
                     /translation="MTQNEAFMEHAPIINEKRCTRCGRCVEVCPKGIVVLRDDAVAYG
                     RAGCMRCAHCYAVCAFDAISFDPPLVAPSFTSFPYAEEMVQPGKVRPGDLVNLLRSRR
                     SVRRYAERPVPDEAMADLVAFAVTAPSGSNCQRWEFTVVNGRAKVEALGRSIGDFFTR
                     LNRLVANPIVRALSVLVAGMKLVRYYRDHYASVAWGLEQAAAGRDPLFHGAPGLIIIH
                     GRTDGSTPLEDAQYAGYNICLLGHALGLGSCFIGYAVEVLNRDTKLRRSLEIPPGNRV
                     LAVIALGYPAVRFLKPALRNDYTVQWL"
     gene            2407..3522
                     /locus_tag="PKJ16_01500"
     CDS             2407..3522
                     /locus_tag="PKJ16_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45684.1"
                     /translation="MALAQNTISRTRLRELALASLDILRDTPARLEALVAVGRLIERE
                     FGGRLDITAYDGLGDLDVAFVEVLDQILFELNADTGASGGIDEYIVDDLYTRAQAFFL
                     IERGPEHYRKNLHERAITLDDPIIIRHHRLAEFVPRLTAEFAEYPNLRCAILKTLLTF
                     DDDLLLPFYYDIVMGDGDTALKIMALIGLHQCRSRFGNWRTIARTADDELNALVAYVQ
                     AFDPSQPADGGALERPYPLVFTLMFLEQQCITGSDALDFERLFATLCATVRSRAAVQQ
                     LYPHLHETIASIFMHIAPDTLSALLDDKRTLLTFLKVVDLFPLEGFEKILVVINRLGP
                     RFLKTAERTIAAGEVQFEKSGPTLIGYILSLSAAEAL"
     gene            3611..4789
                     /locus_tag="PKJ16_01505"
     CDS             3611..4789
                     /locus_tag="PKJ16_01505"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNV45685.1"
                     /translation="MRDVVIASICRTAVGSFGGSLKDINAVQLGVTAVKEAIKRAGIK
                     PEQVENVVLGNVLQASLGQNPARQILIHAGIPQEVPAMTINKVCASGLRSFSLAAQMI
                     KSGDFDIVVAGGTESMTNAPYALTGARWGYRMGANEVVDLMIKDGLWDAFNQYHMGIT
                     AENVAAKWNVTRKELDEFAAESQRKAEAAVKSGRFKDEIVPVAIPQKKGDPKMFDTDE
                     FPRFGTTAESLAGLKPAFKKDGVVTAGNASGINDGAAAGVVMSAEMAKKLGITPLARI
                     ASYASAGVDPALMGTGPIPASRKTLEKAGWKPSDLDLVEANEAFAAQAVVVNKEMGWD
                     LSKVNVNGGAIAIGHPIGASGARIFTTLVYEMKKRGAKKGIATLCIGGGQGAAIAIEM
                     "
     gene            4889..5287
                     /locus_tag="PKJ16_01510"
     CDS             4889..5287
                     /locus_tag="PKJ16_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HNV45686.1"
                     /translation="MTDYSKASTAELQTPRYAFDTIEAKGHRPIVRWEFPEFQSLCPV
                     SERQDQGTVVVTYRPQEKILESKSVRDYFFAWRNLKNWQEYVTEEIADALFAACEPEW
                     LVVEITWAPRGGIFATTVARRGDRSSIETV"
     gene            5323..6570
                     /gene="purD"
                     /locus_tag="PKJ16_01515"
     CDS             5323..6570
                     /gene="purD"
                     /locus_tag="PKJ16_01515"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HNV45687.1"
                     /translation="MKYLVIGSGGREHALAWRLLADGSADEVFVAPGNGGIEERFRVN
                     IAIDDFAGIERFCRDSAIDIVVVGPEAPLVAGLIDFLTGKKIPAFGPTAAAARLEGSK
                     LFAKFIMEKYGVPTAGHRDFSSKADLLAHIETIADYPIVIKLDGLAAGKGVGIPESKA
                     EAIDFIERNVKDGAKVFVEDFLAGEEASVLGISDGETVLPFIAAQDHKRAYDGDRGPN
                     TGGMGAYAPAPVARDAVLERVRREVLVPVVQGMKAEGCPFKGILYAGVIIDGDSIKVL
                     EFNVRFGDPEAQVILPLLNGRLGDFLQASIAGSLHECELSFKRMSAITVVMASGGYPD
                     DYRKGIPITGLDAVSEDLIVFHAGTRMENGACVTSGGRVLNVTALGESFQAARDTVYG
                     EIGKIRFEGAHFRKDIGHRAMGR"
     gene            6666..8987
                     /locus_tag="PKJ16_01520"
     CDS             6666..8987
                     /locus_tag="PKJ16_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV45688.1"
                     /translation="MRPLEILRALLNPPAATVEDRDRKRLFTIYLLLLILPLIIFGTD
                     QIRTGHLDYGIIDYCLAAVLTFFIGLLRRLKNGRGVFRITLALLVSLLTYWLVTGAVD
                     GYASVWVLSFPPFAFFLMGRREGFAWTAIMSLITLVVFVNPGSAVTCFAYPPTLATRH
                     IFALFIIILFTYNYEAVRERFKNAMEGEQANLHAEKERLALASGDLEAVNRRLADEIA
                     IRRHAEEELRAHRDRLEELVGERTLQLKSNVDKLAASEARYRLLAENVTDLIWTMDLE
                     LHFTFMTPSVTSMFGFSVEEAMALPIERWSTPESNERAMREYLTHLELEKSGSADPTR
                     FAVFQVEQFKKDGSTLWVEIKTSFLRDTSGRAIGFVGVTRDISERVQAQRETEKIQEQ
                     LAQAQKMEALGTLVGGLAHDFNNILIGIMGSYDLLGMLLENNCLRDKDEIDQYLRLGA
                     DSARRAADLIKQLLALSKKHGIALTPVDITESLRHIRDICRNSFPKSVALDFRIAERP
                     LVVMGDMVQIEQVLLNLCINASHAMTVMRDPSERQGGVLTVEADEIGPEADGGRGRIR
                     IQISDTGVGMDAETRQRIFEPYFSKKERHDGSGLGLTISYNIVHSHGGRIIVYSEPGA
                     GSRFTLYLPAHEAAIVPTGGEIAQDIVRGSGTVLVIDDEPSVLATARGFLERCGYEVL
                     TAESAEGGIALFRETHGRVSAVLIDLSMPVKSGLEVFRELREIDPSVRALLSSGMLDA
                     ESKNIALALGIREFVNKPYLARELSHKIHAVIG"
     gene            complement(9060..9401)
                     /locus_tag="PKJ16_01525"
     CDS             complement(9060..9401)
                     /locus_tag="PKJ16_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNV45689.1"
                     /translation="MMKRYAVVWTQTAIEDLDSILDHVYFENPENALRILRKIKDRCA
                     LLHGNPSQGRIVPELNCHGIVTYREIIISPWRVLYRIANTCVYVLAVIDGRRNLEDIL
                     LSRIIRGGHHV"
     gene            complement(9398..9670)
                     /locus_tag="PKJ16_01530"
     CDS             complement(9398..9670)
                     /locus_tag="PKJ16_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006366198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNV45690.1"
                     /translation="MNIKEDIRPISYVKAHAADILSQISKTRRPLYVTQNGEAKAVIL
                     DTDSYQEMQKALGLLKIIAQGENDVRRGRLVPQEDVFSKVEKKMKK"
     gene            complement(9891..10406)
                     /locus_tag="PKJ16_01535"
     CDS             complement(9891..10406)
                     /locus_tag="PKJ16_01535"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV45691.1"
                     /translation="MRSRRRNLQRRREGMKSGHGKERALKALNHFSQRERDFMRTVNH
                     GISRQIIDFCVRNRAGTIHMEALTADVKKNAFLTAYWTYYQLQQMVEYKAAEHGIVVR
                     YINPKNTSRMCSACGHVDAANRPTQKDFRCLQCGHEAFADYNASVNIARSSAFVKTGE
                     LIEEEEEEEAG"
     gene            complement(10449..11147)
                     /locus_tag="PKJ16_01540"
     CDS             complement(10449..11147)
                     /locus_tag="PKJ16_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45692.1"
                     /translation="MATQQTLTRTAPRIKLVIADDEKYKEHLYWLGCVGNDLRNLRND
                     VMRECVSYLQKQIDGEPLPVNEKGKTIDYRTYAGQRRKKYDYANGTLCNWTEFLTVKT
                     FKQYKKQILAGKANIPMFKDKSIPIYGKGSRLWVENGEFFLKPPAMNGIVFKFVNPEK
                     NKSLAAILERVLSGEYSFDFSKIIKDDKFWYLHLSYTFGREPDPNLDPTVVCGIDLGW
                     VVPAYCGLNEGPQR"
     gene            complement(11147..11632)
                     /locus_tag="PKJ16_01545"
     CDS             complement(11147..11632)
                     /locus_tag="PKJ16_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45693.1"
                     /translation="MKVSIDTNCIISLFHDSDDIHGPMQCINGFFQQGKISLFVSIKA
                     LDELKEEKPREFALTLPKLPSYPISKWGEQVGSWCATNGSWKEASQVEDVHRYVKCVL
                     SAKMGLRDRQIVFDSIYGNMDALLTRDKKILSERSSGLLGESFHLKVYSPIEFVELLS
                     A"
     gene            12321..12536
                     /locus_tag="PKJ16_01550"
     CDS             12321..12536
                     /locus_tag="PKJ16_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45694.1"
                     /translation="MSVYTFDSFEEARHALWEFDPGPEYYCRVARLFALAFRIAPPRC
                     RRGIRAYASIEEAQKDRFHFQTGLRAP"
     gene            12555..14036
                     /locus_tag="PKJ16_01555"
     CDS             12555..14036
                     /locus_tag="PKJ16_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV45695.1"
                     /translation="MKRAIVIALIASTLLAAETAVRKFIPLPEPVNSPENDFAPSFTL
                     DGNTMVFNSKRGNTLQDIYICHRTDGRWGVPRPIDAINSPFNDETPFITADGKVILFA
                     SDRDGSIEAPRDAQGRVRVSYDLYWAQFYDGAWTRPMRLPGYVNTAMHERAPSLGPDK
                     RSLYYTAWPFGNPAASRIMRAEIAGNIFTNIKTLPNPINTNHHETAFIPDMHGNGFYF
                     SSTRGGGYGGWDIYHVEFKDGMWSTPVNLGPEVNSKQNDFFYTRIGADSYLCSDRPGG
                     AGKYDIWHRAVPQGIPMAALVRNKATRAPMSSNVRIAARRPRQADAIDRILSGRDGML
                     RHELPAETSAAEVTVIEPGYLPFYRTYSPEEIKKGLAIIELVPIEKDISFDVNEIYFD
                     YNLHDIKEESFRVLDAVVEFMKKNPRVRFRVIGHTDLHGTPEYNLKLSLRRANAVREY
                     LISKGIAERRLEIEGAGMSRPKINQKGREADEQNRRTEFKVIE"
     gene            14066..14917
                     /locus_tag="PKJ16_01560"
     CDS             14066..14917
                     /locus_tag="PKJ16_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HNV45696.1"
                     /translation="MNSNRAIISLILVLFVRHGALAIDRTAPFAARIAHTAKDGNVTV
                     HADSSLDSRFAASHAAHAAQVWRYFSSIFARTPGSSVEFFYTKDQRLFETILKRYPVI
                     VIPGGRQLTTHWEGNHRVWFIIPYTMPDFGTQLHELSHDFLYATWPRSEEHPWIKEGL
                     GMYFESGRISEQGRFSVERPFPSYVVLFRDWQAKGALIPLRRLLTMPRDEFYRSDHTK
                     TYSQSMMLVFYLWEKHPAMMRGVLAGFNNRTITTNAQLIDFILRSTGLSLVTLEREYI
                     PYGTAAR"
     gene            complement(14925..15584)
                     /locus_tag="PKJ16_01565"
     CDS             complement(14925..15584)
                     /locus_tag="PKJ16_01565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV45697.1"
                     /translation="MGYRAVIFDLDGTLLDTLADLGNAMNATLIAHGFPTHNADDYRR
                     IIGGGMASFVEKAFPSTGWDRARIGEIVAHVRAEYATRWDEYTAPYPGIEEMLDALAA
                     RGIRMSVLSNKVDDFTKRIVASLLPRWRFDAVYGERAGIPVKPDPVSALEIARIMEVD
                     PASCVFVGDSGIDMKTGRAAGMFPVGVAWGFRGEEELRASGAGRIIHAPGELVSMFDA
                     D"
     gene            complement(15584..16999)
                     /locus_tag="PKJ16_01570"
     CDS             complement(15584..16999)
                     /locus_tag="PKJ16_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017316439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HNV45698.1"
                     /translation="MTQRKRTIRSKKETKSAVARLLEGQRAFFDGGVTRDVDARLRQL
                     TALRRAIVAGEREILAALAADLRKPAYEAYLAEIVFLYHEIDFTMKHLGRWAKPRRAG
                     APSILRPSENFVVPEPYGAALIIGPWNYPFQLTIAPLVGAIAAGNTAILKPSELAPAT
                     AKVIADLIGRTFDPEFVAVVTGGVDATTALLRERFDHIFFTGGTAVGRIIMEAAAKHL
                     TPVTLELGGKSPTIVLADADIETAARKIAWGKFINAGQTCLAPDYVLVDERVRTRFVD
                     ALGRAIGNFYGDDPQASPDYARIITTRHFARLRGLMGPHAPRIGGRTDERDRYIAPTV
                     IENVPLDDPLMREEIFGPLLPVLSVGSLDEALGIVRSMSKPLALYLFTGDRAAERRVI
                     RETSSGAVCVNDVVIHVSNYRLPFGGVGESGMGAYHGQYSFEAFSHLKPVMKRTRLFD
                     MPLRYPPYRGSAMNIVRRIFG"
     gene            complement(17089..17655)
                     /gene="thpR"
                     /locus_tag="PKJ16_01575"
     CDS             complement(17089..17655)
                     /gene="thpR"
                     /locus_tag="PKJ16_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HNV45699.1"
                     /translation="MARLFAALTVDAAAADALAPARALLEPFSSIIKTVAPQSYHITL
                     KFFGECDGARARAIEAALDRISPPGALAYALCGLGAFPSSRNASAIWCGIRADLGPMR
                     RLAGEIEDLAERLGFPREGRPFAPHLTLARVRRGRRPPAALAACIDGNARTLFGESAF
                     DRMALFSSRLRPEGPEYTPLRVITLPAP"
     gene            complement(17662..17964)
                     /gene="gatC"
                     /locus_tag="PKJ16_01580"
     CDS             complement(17662..17964)
                     /gene="gatC"
                     /locus_tag="PKJ16_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HNV45700.1"
                     /translation="MKIDREQIERVAELARLELTEDERQEFSKQLSDILSYVEKINEL
                     DTDAIQPADHIVDLRNVFREDAPGPSLPRAELERMAPRFEKGHVVVPRIIDEKPSH"
     gene            complement(17954..18676)
                     /locus_tag="PKJ16_01585"
     CDS             complement(17954..18676)
                     /locus_tag="PKJ16_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45701.1"
                     /translation="MRMAGLLMVVAVIASAGCARRYGIEHGVRPADGGASATGARCRI
                     IQDAGVELAVEYVDAVRWETLLSHRRFRKKRPRDSTSRMPRYHLFHVVIRNAGRAPLR
                     VPDIILVAGALRQAPIARDELKKRGKSKAFSAVQLARLVEPMRLEGGARCPAKIDYDR
                     DAIGYGLPVIAPGETVARIVAFEWLPVEIRSCLLEVVLAPPGGVGAKKVIAFAYRRYE
                     YRTHGTVFLDPERASKEKGDED"
     gene            18807..19526
                     /locus_tag="PKJ16_01590"
     CDS             18807..19526
                     /locus_tag="PKJ16_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV45702.1"
                     /translation="MEVRIGLFSKNNELIESFTAGSEPCATRIFRNLETPRNEQYDLL
                     AVDADLFDSSDVIQFHLSRVRKKFKSTPVLLIPSVKHLDGIDMSWFFDDIILYPFRKG
                     ELAVRIKRLLWQNKSREDDAVIELGSVTINPKEYLVYHDNEKVELTYKEFELLRLMVQ
                     NRGVVFSRKELLANVWGLDYIGGTRTVDVHIRRLRGKLGDDFNSIIETVRNVGYRCIQ
                     EGETAVPEQEQSDDPEEAADA"
     gene            19667..20170
                     /locus_tag="PKJ16_01595"
     CDS             19667..20170
                     /locus_tag="PKJ16_01595"
                     /inference="COORDINATES: protein motif:HMM:NF015817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HNV45703.1"
                     /translation="MNQEQKIEKLVDCILSDDLSEYRLLLKNLKRKLPFFTGSRNIAA
                     ALLKQHLGDISNLDLTLNSLHQRAEDGKVLFEDIKDGKARLFINIGKNQKISPGDLIR
                     EIVKRSGIDGKFVGKIDIHSTYSFFEIPEKYAELVLVSLDKSKIRGVTITVEPAKRRR
                     KGKDNDE"
     gene            20193..21035
                     /locus_tag="PKJ16_01600"
     CDS             20193..21035
                     /locus_tag="PKJ16_01600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV45704.1"
                     /translation="MTASCPLCRSRRRKPLPFTSFVRCRDCGAVANEAYRPLAYSDAY
                     FTEDYRAQYGRTYEEDFDAIYASSRSRLAFLFDRFPRPDRSRLRLLDIGCALGFFLQA
                     ARDAGVGALEGIEISPYAASIARGRLGCTVHERPFGPGAVSGRFDIITAWYFIEHCPD
                     PRTTVEAIYDLLAPGGLFAFASPSAFGPLFRFDRARWIETHPVDHRVDFTPHAARRLL
                     AQTGFRAPHTRPGAIHPERIMSPDAFGYRPVRFCYAAFSRLASFSDTLEVYAQKPPVS
                     RPVA"
     gene            21199..21357
                     /gene="rpmH"
                     /locus_tag="PKJ16_01605"
     CDS             21199..21357
                     /gene="rpmH"
                     /locus_tag="PKJ16_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HNV45705.1"
                     /translation="MKRTFQPSITKRLRTHGFMARMETPGGQEVIKRRRRKGRKKLTV
                     SDERKGAK"
     gene            21368..21763
                     /gene="rnpA"
                     /locus_tag="PKJ16_01610"
     CDS             21368..21763
                     /gene="rnpA"
                     /locus_tag="PKJ16_01610"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HNV45706.1"
                     /translation="MKRIVPLKGRKTFQEVFKRGKRYAAEGVRISVLRLTRDREQQVR
                     RIYREFDRGRDIVTGITISRKYGTAVERNRMKRRTRAIFRELLPGMAPGFITVAHYDA
                     RGEALRYAVVRDRLAALLKRAGVYETHED"
     gene            21753..21980
                     /gene="yidD"
                     /locus_tag="PKJ16_01615"
     CDS             21753..21980
                     /gene="yidD"
                     /locus_tag="PKJ16_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV45707.1"
                     /translation="MKINGLFIALAIGLIQLYQLLLSPLLPPACRFYPSCSAYAIEAL
                     RKFGLVKGLALAAYRILRCNPLCRGGYDPVP"
     gene            22099..23802
                     /gene="yidC"
                     /locus_tag="PKJ16_01620"
     CDS             22099..23802
                     /gene="yidC"
                     /locus_tag="PKJ16_01620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HNV45708.1"
                     /translation="MEKKDILRAVVAIVLSLAVWVGWMYFFKDHMPQEQKPQQPAATD
                     AAHKAPGDASRQAAPHATGPATPAARPHAVLAAAPAGVEAKDIELKTGVYSVVFTTRG
                     AAIKSFTYAAKKVELVVPKSKYNATGLFDFALHISEDEFLNGSALDSMIWSHAVKENT
                     VVFSATATMQGLPVRIEKVYTLIPGKHHFTVETRIANLGRTPLPLPNKYLIMSPSDFM
                     GPALDFENTYNQVNSMFQINGGFEKPNKGGGFFSKNEGPTAVQKGAVTWVGVSSRYFL
                     IVMLANGFSGTGAIYDNRADRGFRAGMYVPVDSIQPGTTLARSFKVYVGEKNKDLLAE
                     VDKSMVDATDISRWIEPIRDFLLWCLMKINILFGNFGWSLVVFSIVTKLIFLPLTMKS
                     TESMKKMQDLNPKIQEIREKFKDKPDVVNKKIMEMYKKEKVNPLSGCLPLLLQMPFFF
                     ALYSALINSVDLWQAPFVLWITDLSLPDTVYAISGFNINILPIFMTATTYLQQRMTTT
                     EGTSQQQKMMMFMPLIFIVIFWNMPSGLVLYWTMQNVLQIGHQLYINKRGDKKKESPS
                     A"
     gene            23840..24478
                     /gene="jag"
                     /locus_tag="PKJ16_01625"
     CDS             23840..24478
                     /gene="jag"
                     /locus_tag="PKJ16_01625"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HNV45709.1"
                     /translation="MKVLELEGKTVDDATKRALKEFGLNEEDVKIEIVDQGKSGIFGF
                     GVSRPAKIRVYYSENTMDVGGLASEVLVTILSKMDIDGRVKDLKEGENKVYIEIDSAQ
                     SGLIIGRKGRTLESLQFIVNLIVNHQTNTEKKIILDIESYRAKREKALRKISKEVALK
                     VVRTGRPWTLEPMNPFERRLIHLTLQNDTRVTTKSEGEGIYRKVKVSPRGPK"
     gene            24480..25835
                     /gene="mnmE"
                     /locus_tag="PKJ16_01630"
     CDS             24480..25835
                     /gene="mnmE"
                     /locus_tag="PKJ16_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HNV45710.1"
                     /translation="MSPDTICAPASGPLAAPIAVIRVSGPGAHGAVAAVFSRPDALLP
                     RRAVFGSILDGETLVDDVVMVCYAAPASFTGEDSAEIFCHGNHLIVRRILSLLHARGV
                     RLAEPGEFSRRAFENGKIDLTAAEAINHIITARSDWEIEAALAQAHGSLRDAVRRLRE
                     DLVAYRADIECAIDFAEEEVPQLSREESLERITSIAAGIDAIRRRCATGERLARGIDA
                     PLIGRPNVGKSSILNCVINAERALVSHIPGTTRDLIRETVQFAGVPVNLIDTAGIGAP
                     GCELERMGIELSQRKIREAGIVIMVLDAVDGLTDDDRTILDMVADRPTVYLANKADLA
                     ATDDIAAIGRSLGRPVIPFSAVRATGLDALEAAIGRLVRDSIVEPERGFLADHRIMAL
                     LDEAIGTAQAVRTLVAEGRPPEIVAFELQTLADTLGAVTGEITPEDVLTSVFSRFCIG
                     K"
     gene            complement(25847..28684)
                     /locus_tag="PKJ16_01635"
     CDS             complement(25847..28684)
                     /locus_tag="PKJ16_01635"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HNV45711.1"
                     /translation="MADIIARIAQNKERFQGFNALKLRKFLASLSSKNLSDLIDIIPV
                     LITGNHPQLPGFVPEYDPVGIYGYRPAPRAINLLASLFPGAGLGDARPRRQLIEFFAI
                     IGSTGSIAYTEDSDIDFWICLDESALEKRELDAMRSKLRLIEHWITENYNIETHFYVN
                     DTENVKVDIFDADEVDITGKALGKLLKDELYRSSILLNGKIPFWWVVPAGTDDARYAA
                     AMRAVPKEEFAREYIDLGNLHVINADDFIGAGLFQILKSLGNPFKSIIKIGLIEKYLL
                     DAAGERDFLCTIVKRNVHEGKLETEQVDPYILMFRLVYDYFSRNIMREDPTADEILKT
                     CFYLKVEPNLSQYVTLQQGQMVRTVTAGGGKVALQRGSSIDEKIAIMNAMVKEWRWDE
                     DRLREIDNFRGWDITAVNTFWNDITKLVLKSYKRITASVGRASIAQKFTDAEIKFFAR
                     KIQSSLAPSANKIRPAVIFKDNPIEKQLTVESVSEAGGRIAWLLTKGFIGYNVSSTML
                     IMHKEPSLIGMLAWISINRLYLKDTTRLEIKSRYQLVENNFLRELLNDLTLHFSIKKL
                     HIRNRYFLVDPFPILNFIIFNLDSKYTRGFEEFYYLYHNSWGETVFERFTREIDLAAV
                     LERILNGALAHKESFDASVWLTSSYPYKSGGAFKAYQSLFRTVYGVFLAEGERSGRKF
                     LTMLGNNFVLYSCRRHQGGDAVECRVFDTEVKMLYSLINLSGMNATLTVDERNPELNY
                     LRAIVERRAPGAINIFFQRERKYCYFFIIDECGSLSFYRKGADRFQLYLARLHAFADN
                     AAAHVVSHNPHSPLATAEKRVRLYELTRGARNAATVTEVGPEVYDAVAGAARGVVPLR
                     LSLSLLDNGETGYRFTLPDGGLSDTYGRADIPSVAREIAVLMDTVPGYGYYATDITIE
                     NIGVAMYRMFTSFSFAEKNRFELLVEKGLGG"
     gene            complement(28705..29940)
                     /locus_tag="PKJ16_01640"
     CDS             complement(28705..29940)
                     /locus_tag="PKJ16_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV45712.1"
                     /translation="MNTADGQDKGTRTNRIDLRFMKAGTINSFDLKDEYGTVVIEAYT
                     ALDQALINHLLGKGVQHLYYTEPEKHDAAKSAGGIDLATNAVSETLQRESIDQARDIL
                     EYVRESFNVDPGRTINTAMLAKSRGVVEKILDGYEKNKNAAFSPVAKLKSMDEYTLVH
                     SSNVSTLAAILGTRLELSKDIRIGMGVGGLFHDIGKTSLSDAIVLKAGKLSDEEFDRM
                     KEHPHVGYKFVEGNPSMHDIEKRIILLHHESPDANGYPFGMDLEHYQKNVPREIRLLT
                     ICDVYSALTTERPYGRPYAPREALRILLNSIYAPYKKKYRFLPNDLRDFIRSMGFILN
                     NGEFFFDRGDVVRLNSGEIGVIEGMNRLYPLNPVIRVIADRESRKLKRPIVVDMLKEY
                     NSYIAHLFDKGAGAVAPSA"
     gene            30132..30830
                     /locus_tag="PKJ16_01645"
     CDS             30132..30830
                     /locus_tag="PKJ16_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNV45713.1"
                     /translation="MRTILFAIGALAVAGIVAASPLAASGKWKVVKQSDGITISTRPV
                     EGSPVDEFRGVTTINARIEVIGEVLRDVPANVEWMADCKEARVISRKDKDNLVIYFVQ
                     GAPGPVSNRDFIVRVNTTKNYDKGVVIVNLKAIAEPLVPVNPKYIRITDLTGQYILQY
                     IDRERTKVIYTVRANPGGSLPTWIANYASKDTPFKTLAGLRRQSAKAKYVELGNNSED
                     KKKIEERLKKGGLK"
     gene            30944..31510
                     /locus_tag="PKJ16_01650"
     CDS             30944..31510
                     /locus_tag="PKJ16_01650"
                     /inference="COORDINATES: protein motif:HMM:NF025637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNV45714.1"
                     /translation="MLNSDVLVLNSGFIPIRITSVRDAVCLIAAHRAVPVIEDDEYVR
                     SPSISIRIPSVISVLGYNSFPQRRVVFSKLNVIYRDDMLCQYCGSRFNMKDLTVDHVI
                     PRSRWESVTGRDQGDGFSTWHNLVCACRWCNNRKGSRLLHELGWALLREPFEPEYMPY
                     IFVSFERAKQKGWLPFCNFNVRIIGVVE"
     gene            complement(31947..32561)
                     /locus_tag="PKJ16_01655"
     CDS             complement(31947..32561)
                     /locus_tag="PKJ16_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45715.1"
                     /translation="MATAARGWQAFTVFLEMVKIEHSIFALPFALIGMMWGSMQKFNT
                     PWPGFWIFGWIVVAMVLMRLGYTGILPYVDPVIVIALIVVSFPVPLKVVLVEFRRLLL
                     VSPENSIEDEVKGLLRDVIEHYGMIDVQVWALKSGRTQYFFLYSSLREEHTTIRRLDE
                     IRLAIFRELAKQYPKFWADIMFTRINPENTTESLLESADVRVDR"
BASE COUNT         6390 a        10436 c         9758 g         6153 t
ORIGIN      
        1 ttgggcatgg gaagacggcg ccaccgcgat cggcatcaac gtgagtgctg cacgattcgc
       61 gtcatatccc actatgatgg ttttcaactc gaagctgtac gcgctgtggg aggaggggaa
      121 catatccgcc gtccagcaga ttcggttcgg cgtctatacc ggcggcggcg tctggaacaa
      181 gctaaatacg gatgtcaact gcgggatcaa ttacaactgc gcacagaacg cccgccggcc
      241 ctccatggcg gtgtgctggg gaagtctcca tttggcctgg tacgagacaa acggctccgg
      301 ttttaaccag atccaggtga tgacttacaa tggcaatgac gcaagtcccg acggcatatc
      361 gatcacagga caatcgaatc ccgaatacgg tctgaattac aatccgtcga tgaacgccgc
      421 ttatccggtt cttgtcggtt tgaatgataa cctctacgtt gcctggtacg aaagcaacgg
      481 gaccgcaaat cagatacgag tggccgccgc gagctggtac tgatcgcttt tacaaataat
      541 ttcttgacaa agagagattt tcgcgttctc atcgagtcat ggtaaagctg gacaatccct
      601 atcccgacag ctcgtaccgc tccgttctgg cgcccgtggc gctgctgttt gcgctgggct
      661 tcgtgtgctg ccgggtgagc tttttctcgg ggctctatca gtatgcgatc ctgctgttcg
      721 tcgtgatcct cgtggcgtgc tttttcgacc gcgggctcat cgggcgcgac cggctctttt
      781 cgtggccgtc gtccgtgtcg catctggtgg tggggatcac ggcggccgtg gcgctctttt
      841 tcgccgtgta catcctgctt cgcctcacgc ggaacatcgc cgccttcagc atctatttta
      901 cggcgggcgg ggcgctgtcg aagtgggagc tcttcgcgac ggcggcggtg ctcctccccg
      961 tcatcgagga ggcgctcttc cggggattta tccagcacaa tctggccaac agcttcggcg
     1021 cccacacggg ccttgcgctc ggttcggcaa tcgcgatcct gtttagcctg ctgttcacgc
     1081 gcgccgtctg ggtggcgctc cccctcgcgg cgctcacgct cctgacgggt tttctctaca
     1141 tgcgctaccg gtcgctcgcg gtgtccatcg ccgcgcacgg atccttcgcg ctcctgctct
     1201 acgtcttcgt cgtgtgatta cagccactgc accgtatagt cgttcctgag ggcgggcttg
     1261 agaaagcgca cggccgggta gccgagcgcg atcaccgcga gcacgcggtt ccccggcggg
     1321 atctccaggc tccgccgcag cttcgtatcg cggttgagca cctcgacggc gtacccgatg
     1381 aaacagctcc ccagccccag cgcatgcccg agcaggcaga tattgtaccc cgcgtactgc
     1441 gcgtcctcca gcggcgtgct cccgtccgtc cgcccatgta tgatgatgag ccccggcgcg
     1501 ccgtgaaaca gcgggtcgcg ccccgcggcc gcctgctcga gtccccatgc cacgctcgcg
     1561 tagtggtcgc gataatagcg gaccagcttc atccccgcca cgagcaccga aagcgcgcgc
     1621 acgattggat tcgcgaccag ccggttgagc cgcgtgaaaa agtccccgat ggagcggccc
     1681 agcgcctcga ccttcgcgcg gccgttcacg acggtgaact cccagcgctg gcagttgctt
     1741 cccgacgggg ccgtcaccgc gaaggcgacc aggtccgcca tcgcctcgtc gggaacggga
     1801 cgttcggcat agcgccgcac gctgcgccgc gagcggagca ggttcaccag gtcgcccggc
     1861 cggaccttcc cgggctgcac catttcctcg gcgtagggaa agctcgtaaa ggacggcgcg
     1921 accagcggcg gatcgaagct gatggcgtcg aaggcgcaca ccgcgtaaca gtgcgcgcag
     1981 cgcatgcatc ccgcgcgccc ataggcgacc gcgtcgtcgc gcagcactac aatgcccttg
     2041 gggcacacct cgacgcaccg cccgcagcgg gtgcatcgct tctcgttgat gatgggcgcg
     2101 tgctccatga acgcctcgtt ctgtgtcatc acgcacttcc ccctgaaaaa agtgctccat
     2161 ttcgaatgat gatggcgatt gcacacaatg cgtccatgaa tttcaatcca ttaatccacc
     2221 ggggatccgt ttcgcatcga ccgtctccgt ccgcgagcgc gtccgatgat ccgccatcgc
     2281 cgattctcgg tgcgatgcgc acgcgtcccg tgacgcggga aaaatttctc gacagcccgg
     2341 acccccggta tccaatggcc atcatcagca caccatcgtg agcgaacaca cagaatcgaa
     2401 gccttgatgg cactggcaca gaacacaatc agccgcaccc ggctccgcga gctggcgctg
     2461 gcctccctgg acatcctccg ggacacgccc gcgcggctcg aggcgctcgt cgccgtcggg
     2521 cggctcatcg agcgcgagtt cggcggacgg ctggacatca ccgcctacga cggtctgggc
     2581 gacctcgacg tcgccttcgt cgaggtgctc gaccagatcc tcttcgagct caatgccgac
     2641 accggcgcga gcggcggcat cgacgagtac atcgtcgacg atctctacac gcgcgcgcag
     2701 gcgttcttcc tcatcgagcg cggcccggag cactatcgca agaacctgca cgagcgcgcc
     2761 atcacgctgg acgaccccat catcatccgg caccaccgcc tcgccgagtt cgtgccgcgg
     2821 ctcaccgccg agttcgccga atacccgaac ctgcgctgcg cgatcctgaa gacgctgctc
     2881 accttcgacg acgacctcct cctcccgttc tactacgaca tcgtgatggg cgacggcgac
     2941 acggccctga agatcatggc gctcatcggg ctgcaccagt gccgttcgcg cttcgggaac
     3001 tggcgcacca tcgcgcgcac cgcggacgac gagctcaacg ccctggtcgc ctacgtacag
     3061 gccttcgatc cctcccagcc cgcggatggc ggcgcgctgg agcggcccta tccgctcgtg
     3121 ttcacgctca tgttcctgga acaacagtgc atcaccggat cggacgccct cgacttcgag
     3181 cgcctctttg cgacgttgtg cgcgaccgta cgctcgcgcg cggccgtgca gcagctctat
     3241 ccccacctgc acgagacgat cgcgagcatc ttcatgcaca tcgcgcccga cacgctctcc
     3301 gcgctcctgg acgacaagcg cacgctcctc accttcctca aggtggtgga cctgttcccc
     3361 ctcgagggtt tcgagaagat cctggtggtc atcaaccgcc tgggcccgcg cttcctgaag
     3421 accgccgagc gcacgatcgc cgccggcgag gtgcagttcg agaaaagcgg cccgacgctc
     3481 atcggctaca tcctgtcact ctccgccgcc gaggcgctgt aggaattttt gtttacaaat
     3541 aaccgatggc ggaaatgatg ttcgccatcg ccatcatacg ccaatcacaa cacacgagga
     3601 gaggggaatc atgagagacg tcgtcatcgc atcgatatgc cgcaccgccg tgggatcgtt
     3661 cggcggcagc ctcaaggaca tcaacgccgt ccagctcggc gtgaccgccg taaaggaggc
     3721 gatcaagcgc gccgggatca agcccgaaca agtcgaaaac gtcgtcctgg gcaacgtgct
     3781 ccaggcctcc ctgggtcaga acccggcccg acaaatcctc atccacgccg gcatcccgca
     3841 agaggtcccg gccatgacca tcaacaaggt ctgcgcctcg ggcctgcgtt cattctcact
     3901 ggcagcgcag atgatcaaat cgggagattt cgacatcgtc gtcgccggcg gcaccgaaag
     3961 catgaccaac gccccctacg cgctcaccgg cgcccgctgg ggctaccgca tgggcgcaaa
     4021 cgaggtcgtg gatctcatga tcaaagacgg tctctgggac gccttcaacc aatatcacat
     4081 gggcattacc gccgagaacg tcgcggccaa gtggaacgtc acccggaagg agctcgacga
     4141 gttcgccgcg gagagccagc ggaaggccga ggccgccgtc aagtccggcc gcttcaagga
     4201 cgagatcgtt cccgtggcca tcccgcagaa gaagggcgat cccaagatgt tcgacaccga
     4261 cgagttcccg cgcttcggca ctaccgccga atcgctcgcc ggcctcaagc ccgccttcaa
     4321 gaaggacggg gtcgtgaccg ccggaaacgc ctcgggcatc aatgacggcg cggccgccgg
     4381 cgtggtcatg tccgccgaga tggccaagaa gctgggaatc accccgctcg cgcggatcgc
     4441 atcgtacgcc tccgccggcg tggaccccgc cctcatgggc accggtccca tcccggccag
     4501 ccgcaagacg cttgagaagg ccggctggaa gccgagcgac ctcgacctcg tcgaggcgaa
     4561 tgaggccttc gccgcgcagg ccgtggtggt caacaaggag atgggttggg acctctcgaa
     4621 ggtgaacgtg aacggcggcg ccatcgcgat cggacatccc atcggcgcct cgggcgcgcg
     4681 tatcttcacc acgctcgtgt acgagatgaa gaaacgcggc gcaaagaagg gcatcgctac
     4741 gctgtgcatc ggcgggggac agggcgcggc catcgccatc gagatgtaga ggccggtcgc
     4801 gtttttcagt tgcaattaca cgaatcccat tctctggtgc aaccggggaa tgggattttt
     4861 tattttatga cgccgcacac ggacatctat gacggattat tcgaaggcat cgaccgccga
     4921 actgcagacg ccgcgctatg catttgacac catcgaggcg aaggggcacc ggcccatcgt
     4981 ccgctgggag tttcccgaat tccagagcct gtgtccggta tcggagcggc aggaccaggg
     5041 gaccgtggtg gtgacctatc gaccgcagga gaaaattctc gagagcaagt cggtgcgcga
     5101 ttacttcttc gcgtggcgga acctcaagaa ctggcaggag tacgtcaccg aggagatcgc
     5161 cgatgcgctc ttcgccgcct gcgagcccga gtggctcgtc gtcgagatca cctgggcccc
     5221 gcgcggcggg atcttcgcaa caacggtcgc ccggcgcggc gaccgttcca gcatagagac
     5281 cgtctgacgc gtatcgacca cagcgacgaa cgggaggaat acatgaagta cctcgtgatc
     5341 ggatccggcg gccgcgagca cgcgctcgcg tggcggctgc tcgccgacgg cagcgcggac
     5401 gaggtgttcg tcgcgcccgg caacggcggc atcgaggagc gcttccgcgt gaacatcgcc
     5461 atcgacgatt tcgcggggat cgagcgcttc tgccgcgaca gcgccatcga catcgtggtg
     5521 gtgggccccg aggcccccct ggtcgcgggc ctgatcgatt tcctcaccgg gaaaaagatc
     5581 ccggccttcg gccccaccgc cgcggccgcg cggctcgagg gaagcaagct cttcgcgaaa
     5641 ttcatcatgg aaaaatacgg cgttccgacc gccggccacc gcgacttctc ctcgaaggcg
     5701 gaccttctcg cccatatcga aacgatcgcg gactacccga tcgtcatcaa actcgacggc
     5761 ctggccgccg gcaagggcgt gggcattccc gaatcgaaag cggaggcgat tgatttcatc
     5821 gaacggaacg tgaaggacgg cgcgaaggtc ttcgtcgagg atttcctcgc cggcgaggag
     5881 gcctcggtcc tcggcatatc ggacggcgag accgtgctgc ccttcatcgc cgcgcaggac
     5941 cacaagcgcg cgtacgacgg cgaccgcggg ccgaacaccg gcggcatggg cgcctacgcc
     6001 ccggccccgg tggcacgcga cgccgtgctc gagcgcgtcc ggcgcgaggt gctcgttccc
     6061 gtggtgcagg gcatgaaggc ggagggctgc cccttcaagg gcatcctcta cgccggcgtc
     6121 atcatcgacg gggattccat caaggtgctg gagttcaatg tccgcttcgg cgatcccgag
     6181 gcgcaggtga tcctgccgct cctgaacggc cgcctgggcg atttcctcca ggcctccatc
     6241 gccggcagcc ttcacgagtg cgagctttcc tttaagcgca tgagcgccat caccgtggtc
     6301 atggcctcgg gcggataccc ggacgactac cggaaaggaa tccccatcac ggggcttgac
     6361 gcggtgagcg aggacctcat cgttttccac gcgggaacgc ggatggaaaa cggcgcgtgc
     6421 gtcaccagcg gcggacgcgt gctcaacgtg accgcgttag gcgaaagctt tcaggccgca
     6481 cgcgacacgg tgtacggcga gattggcaag atccgcttcg agggcgcgca tttccggaag
     6541 gacatcggcc accgggcgat ggggcgctaa agcccgcggg gcattcccgt ccgccattgc
     6601 gtgcgctcgc gctcgacatg aatccttgat ttctcccctg ggcaccagta aacttcatca
     6661 tggcaatgag accccttgaa atccttcgcg cccttctcaa tccgccggca gcgaccgtcg
     6721 aggaccgcga tcgaaagcgg ctctttacca tttatctgct gcttctcatt cttccgctca
     6781 tcatcttcgg gacggaccag atcaggaccg gccacctcga ttacggaatc atcgattatt
     6841 gcctcgcggc ggttctcacc tttttcatcg ggctgctcag gcgcctgaag aacgggcggg
     6901 gcgttttccg aatcacgctg gcgctcctcg tttccctgct gacctactgg ctcgtgaccg
     6961 gcgcggttga cggatacgca agcgtttggg tgctctcctt tccgccgttc gcgttttttc
     7021 tgatgggccg ccgcgagggc ttcgcgtgga ccgcgatcat gtcgctgatc accctcgtcg
     7081 tgttcgtcaa ccccggttcg gcggtcacgt gtttcgccta cccgcccacc ctggcgaccc
     7141 ggcacatctt cgcgttgttc atcatcatcc tatttaccta caattacgag gccgtgcgcg
     7201 agcgtttcaa gaacgcgatg gagggggaac aggcgaacct gcacgccgaa aaagagcgat
     7261 tggcgctcgc ctcgggcgac ctcgaggccg tgaaccgtcg cctggccgac gagatcgcca
     7321 ttcgacgaca cgccgaggag gagctgcgcg cgcatcgcga ccgcctcgag gaactcgtcg
     7381 gcgaacgcac cctgcaactc aagagcaatg tcgataagct cgcggccagc gaggcgcgct
     7441 accgtctcct cgccgagaac gtgaccgacc tgatctggac gatggacctc gagcttcatt
     7501 tcacctttat gacgccgtcg gtgacgtcga tgttcggctt tagcgtggag gaggcgatgg
     7561 cgcttcccat cgagcgctgg agcacgccgg aatcgaacga gcgcgccatg cgtgaatacc
     7621 tcacccacct cgaattggaa aagagcggtt cggcggaccc gacccgcttc gcggtctttc
     7681 aagtcgaaca gttcaaaaag gacgggtcga ccctctgggt cgagatcaag acctcgtttc
     7741 tgcgcgatac gagcggacgc gccatcggct tcgtgggggt cacgcgcgat atcagcgaac
     7801 gcgtgcaggc acagcgcgaa acggagaaga tccaggagca gctcgctcag gcgcagaaga
     7861 tggaggcgct cggcaccctc gtgggcggcc tcgcgcacga tttcaacaac atcctcatcg
     7921 gcatcatggg cagctacgac ctgttgggca tgctgctcga aaacaactgc ctgcgcgaca
     7981 aggacgagat cgaccagtac ctgcgcctgg gcgcggactc ggcccggcgc gcggcggacc
     8041 tcatcaagca gctgttggcg ctgtcgaaaa agcacggcat cgcccttacg cccgtggaca
     8101 tcaccgagtc gttgcgccat atccgcgaca tctgccgcaa cagcttcccc aaatcggtcg
     8161 cgctcgattt ccgcatcgcg gagcgcccgc tcgtggtcat gggcgatatg gtgcagatcg
     8221 agcaggtgct gctcaacctg tgcatcaacg cctcgcacgc catgaccgtt atgcgcgatc
     8281 catcggaacg ccagggcggc gtcctcacgg tggaggccga cgagatcgga ccggaggcgg
     8341 acggcggacg cggacggatc cgcatccaga tctcggacac cggcgtggga atggacgcgg
     8401 agacccggca gcgcatcttc gagccgtatt tctcgaagaa ggagcgccac gacgggtcgg
     8461 ggctcgggct cacgatatcg tacaacatcg tccactcgca cggtgggagg atcatcgtgt
     8521 attcggaacc gggcgccgga tcgcgcttca cgctgtacct gcccgcccac gaggccgcca
     8581 tcgtcccaac gggcggcgag atcgcccaag acatcgtccg cggctccggc accgttctgg
     8641 tgatcgacga cgagccgtcc gtgctcgcca ccgcgcgcgg gttcctcgaa cggtgcggct
     8701 acgaggtcct caccgccgag agcgcggagg gcggcatcgc cctcttccgg gagacgcacg
     8761 gccgcgtgtc ggccgtcctc atcgaccttt ccatgccggt caaatcgggg ctggaggtgt
     8821 ttcgcgagct gcgggaaatc gacccctccg tgcgcgcgct gctgtcgtcg gggatgctcg
     8881 acgccgaatc gaagaacatc gcgctggcgc tggggatcag ggagttcgtg aacaagccgt
     8941 acctggcgcg cgagctttcg cacaagatcc acgcggtcat cggttgagcg cattcccggg
     9001 cagcgccgta tccacaggga gtccgtagat ccgtcaacct tccgcgattt cgctgcccat
     9061 tacacgtggt gtccgccgcg gataattcta ctcaacaata tatcttcgag atttcttctg
     9121 ccgtcgatga cggcaagaac atacacgcat gtgttcgcga ttcgatataa cacccgccag
     9181 ggggaaatga ttatttcgcg ataggtaaca atgccatgac aattcaattc cgggacgatc
     9241 cttccctggg aaggattccc atgcaacagg gcgcatctgt ctttgatctt tcggagtatt
     9301 cgtaacgcat tttcagggtt ttcaaagtaa acatgatcaa ggatcgaatc gagatcctca
     9361 atcgctgttt gggtccatac tacggcatag cgtttcatta tttcttcatc ttcttctcaa
     9421 ctttcgaaaa aacatcctct tgaggcacca accgaccccg gcgaacgtcg ttttcgccct
     9481 gggcgataat ctttaaaagg cccaaggctt tttgcatttc ttgataacta tcggtatcga
     9541 gtataaccgc tttcgcttct ccgttttgcg tgacatagag cggtctcctg gttttcgata
     9601 tctgcgatag aatgtcggcg gcatgcgcct tgacatacga tatgggacga atatcttcct
     9661 tgatgttcat ggcccccccc aacctggcat cagttcaaat aaagtactaa atacggtcta
     9721 ttttgtcaag aataatgcca cacgcctccc ctgggcctac tgaaataggc cccaaataga
     9781 acggatgggc aatagcagaa gaacatgatg gcaggttgcc gacacggagg gagaaccagt
     9841 cgcgcatcat cgccaccctc atcgatcgcg gcgccgagac gaagtgaggg ctatccggcc
     9901 tcttcctcct cttcttcctc gatgagctcg cccgttttca cgaacgcgct cgagcgggcg
     9961 atgtttacgg acgcgttgta atccgcgaag gcctcgtggc cgcactggag gcagcgaaag
    10021 tccttttgcg tggggcgatt ggccgcgtcg acatggccgc aggccgaaca catcctgctg
    10081 gtgttcttcg ggttgatgta tcgcacgacg atgccgtgtt ccgccgcctt gtactccacc
    10141 atctgctgaa gctggtagta ggtccagtac gccgtgagaa aggcgttctt tttaacgtcc
    10201 gcggtgagcg cctccatatg gatggtcccc gcgcggttcc ggacgcagaa gtcgatgatc
    10261 tgcctgctga tgccgtggtt cacggtgcgc atgaagtcgc gctcgcgctg cgagaaatga
    10321 ttgagcgcct tgagcgcgcg ctcctttccg tgccccgact tcatgccctc ccgccgacgt
    10381 tgaaggttcc gccgacgcga ccgtatctgc gaacggaagt gcctgatgag ctggctgtcg
    10441 cccaggtcct aacgctgcgg tccctcgttg agcccgcaat aggccggcac cacccagccg
    10501 agatcgatcc cgcagacgac ggtcgggtcg aggttcggat cgggctccct cccaaaggta
    10561 tatgaaagat gcagatacca gaacttgtcg tccttgatga tttttgaaaa atcgaaggaa
    10621 tactcccccg atagaacgcg ttcaagaatg gccgcgaggc tcttattctt ctccgggttc
    10681 acgaacttga acacgatgcc gttcatagcc ggcggcttga gaaaaaactc accattctcc
    10741 acccacaacc tgctcccctt gccgtagatg ggaatggact tgtccttgaa catggggatg
    10801 ttcgccttcc cggcgagaat ctgttttttg tactgtttga aggtttttac cgtgagaaac
    10861 tccgtccagt tgcagagcgt cccattggcg taatcgtact tctttcggcg ctgccccgcg
    10921 tatgtgcggt aatctatcgt tttccccttt tcatttaccg gcagcggttc accgtcgatc
    10981 tgtttctgca aatacgagac gcattcacgc atcacgtcat tgcgaaggtt gcggagatcg
    11041 tttcccacgc agcccagcca gtagaggtgc tccttgtatt tttcatcgtc ggcaatgacg
    11101 agcttgatgc ggggggcggt acgggtgagc gtttgttgtg tagccattat gctgatagaa
    11161 gttccacaaa ctcaattggc gagtatactt tcaaatgaaa cgattcaccc agcaaaccgg
    11221 atgatctctc actaagaatt tttttgtccc tcgttaataa cgcgtccatg ttgccgtata
    11281 tgctatcgaa aactatttgc ctgtcccgta gtcccatttt cgcactcaat acacatttca
    11341 cataacgatg cacatcttcc acttgggagg cttctttcca agaaccattt gtggcacacc
    11401 atgaaccgac ctgttccccc cattttgaaa tcggataact tggcaatttt ggcaaggtaa
    11461 gcgcgaattc ccttggtttt tcctctttca actcatccaa tgcttttatg ctcacgaaaa
    11521 gactgatttt accctgttgg aaaaatccgt tgatgcattg catcgggcca tgaatatcat
    11581 ctgaatcatg aaagagtgat atgatgcaat tcgtatcaat tgacactttc atcggagcat
    11641 aatgttacgc agtgcagacc atttccacct catcccccgg cccgccgggc gcttttcgac
    11701 atcctgtctg ctcccgtcac tgctgcatcc tgcagggagc cttctcccaa aggagaaggg
    11761 gtgttcacgc gagagatccc atcccgagga gccctctcct atgggagagg gcgcgccgaa
    11821 ggcggggtga ggtgacgcgc ccgtgggtaa aactgcacct tctccatctc ctcgcgtgag
    11881 ggccgcgcca cgtccgcgca cgcgtgggta aaacgctgtg cagcttgatg ggtcttttct
    11941 ggccccagcc acgtccgcgc acccgtgggt aaaaccctaa aagtccgtat agtgcgcagt
    12001 cagtgagtgt tgcggacgcc attccgggac ggccgtactt ctcaccctca ggccgcatgg
    12061 acggcctctg cgggtttccc cgcatcgggt gcgatcatgg accgctcgcg cgagtcggtc
    12121 aaatactatt tcgccaatcc acctcacgac ccccatcgtc atgcgaccca taatatcgtt
    12181 gacggcatca tccgcccatg acacggtatc gtcatcatgg aatccgggat gagcctgttc
    12241 gaccgtttca tggacgtact cgacgacaaa tccgacgcgc agtttcttcg cgtactgctc
    12301 gagaaaacgg aaaaaacctg atgtccgtat acacattcga ttcctttgaa gaggcccggc
    12361 acgcgctctg ggagttcgat cccggcccgg agtattattg ccgggtcgcg cggctgttcg
    12421 cgctcgcctt tcgaatagcc ccgccgcgct gccggagggg aattcgcgcg tatgccagca
    12481 tcgaggaggc gcagaaggac cgcttccact tccaaacggg gctgcgtgcg ccataagagg
    12541 acggagccac tcacatgaaa cgcgccatcg tcatcgccct tatcgcatcg acgcttctcg
    12601 ccgcggaaac cgccgtgcgg aagttcatcc cgctcccgga gccggtgaac tccccggaga
    12661 acgatttcgc gccgtccttc acgctcgacg gcaataccat ggtcttcaac tcgaagcgcg
    12721 ggaacaccct gcaggacatc tacatatgcc atcggacgga cgggcgctgg ggcgtgccgc
    12781 gacccatcga cgcgatcaac tcgcccttta acgacgagac gcccttcatc acggccgacg
    12841 gcaaggtgat cctcttcgcc tccgaccgcg acggcagcat cgaggcgccg cgcgacgccc
    12901 aggggcgcgt gcgggtgtcc tacgatctct actgggcgca gttctacgac ggcgcctgga
    12961 cgcgcccgat gcgcctgccg ggctatgtga acaccgccat gcacgagcgc gcgcccagcc
    13021 tcgggcccga caagcgctcg ctctactaca ccgcctggcc cttcggcaac ccggcggcct
    13081 cgcgcatcat gcgggcggag atcgcgggga acatcttcac caacattaag acgcttccca
    13141 atcccatcaa caccaaccat cacgagacgg ccttcattcc cgatatgcac ggaaacggct
    13201 tttacttttc ctcgacgcgc ggcggcggat acggcggctg ggacatctac cacgtcgaat
    13261 tcaaggacgg catgtggagc acgccggtga acctgggccc ggaggtgaac tcgaagcaga
    13321 acgatttctt ctacacccgg atcggggccg attcgtacct ctgctcggac cggcccggcg
    13381 gcgcgggaaa gtacgacatc tggcaccgcg ccgtcccgca ggggatcccg atggccgcgc
    13441 tcgtccgcaa taaggcgacg cgcgctccca tgtcgtccaa cgtccgcatc gcggcgcgcc
    13501 gcccccggca ggccgacgcg atcgaccgca tcctttcggg acgcgacggc atgctgcgcc
    13561 acgagctccc cgccgaaacg agcgccgcgg aggtcaccgt gatcgagccg ggctatctgc
    13621 cgttctatcg gacctattcg ccggaggaga tcaaaaaggg cctggcgatc atcgagctgg
    13681 tccccatcga gaaggacatc agcttcgacg tgaacgagat ctacttcgac tataacctgc
    13741 acgacatcaa agaggagtcg ttccgggtgc tcgacgcggt ggtcgagttc atgaagaaaa
    13801 acccgcgcgt ccgcttccgc gtgatcggcc acaccgacct ccacgggacg ccggagtaca
    13861 acctgaagct cagcctccgc cgcgcgaacg ccgtgcgcga gtacctcatc tcgaagggca
    13921 tcgccgagcg ccgcctggag atcgagggcg cgggcatgag ccgcccgaag atcaatcaaa
    13981 agggacgcga ggcggacgag cagaaccgac ggacggagtt caaggtgatc gaataacgat
    14041 cacggcgccg agcgggagag aatagatgaa cagcaatcga gccattatct ccctcatcct
    14101 cgttctattt gttcgtcacg gtgctttggc catcgaccgc accgcgccat ttgcggcgag
    14161 aatcgcgcat acggcgaagg acgggaacgt cacggtgcac gccgattcaa gtctcgattc
    14221 ccgttttgcc gcatctcacg ccgctcatgc agcgcaggta tggcgctatt tttcctctat
    14281 attcgcccga acgccgggat catcggtcga gtttttctat acgaaggatc agcgtctgtt
    14341 cgaaaccatc ctgaagcgat atcccgttat cgtcatcccg ggcggacgac agctcaccac
    14401 tcactgggaa ggcaatcacc gcgtgtggtt catcataccc tacaccatgc cagatttcgg
    14461 cacccagctt cacgaactga gccatgattt tctctatgcg acctggccgc gaagcgaaga
    14521 gcatccatgg ataaaggaag ggctggggat gtattttgaa tcgggacgga tttccgagca
    14581 aggccggttc tcggttgaac gcccatttcc ttcgtacgtc gtgctctttc gtgactggca
    14641 ggccaagggc gccctcattc ccctacggcg gctgctcacc atgccgcggg atgaattcta
    14701 tcggtcggac cacactaaaa cctattcgca gtcgatgatg ctcgttttct atctctggga
    14761 aaaacacccg gcgatgatgc gcggcgtgct cgccggtttc aacaaccgta caatcaccac
    14821 gaacgcgcaa ctgattgatt tcattcttcg atcgaccggc ctgtcgctcg tcacgcttga
    14881 gcgggaatat atcccgtatg ggacggcagc acggtagcgc ctgttcaatc cgcatcaaac
    14941 atcgacacca actcccccgg cgcatggatg atccgtcccg cgccgctcgc gcgcagctcc
    15001 tcctcgccgc gaaaacccca ggcgacgccg accgggaaca ttcccgcggc gcgtcccgtt
    15061 ttcatgtcga tgccgctgtc gcccacgaag acgcacgagg ccggatcgac ctccatgatc
    15121 cgcgcgatct cgagggccga aacgggatcg ggtttcacgg gaatccccgc gcgctcgccg
    15181 tacaccgcgt cgaagcgcca gcgcggaaga agcgatgcga cgatgcgctt cgtgaagtca
    15241 tccaccttgt tggagaggac ggacatccgg ataccgcgcg cggcgagcgc gtccaacatc
    15301 tcctcgatgc cgggataggg cgcggtgtac tcgtcccaac gcgtcgcgta ctcggcgcgg
    15361 acatgcgcga caatctcacc gatgcgcgcg cggtcccatc ccgtcgacgg gaaggccttc
    15421 tcgacgaacg aggccatgcc gccgccgatg atccgccggt agtcgtccgc gttgtgcgtg
    15481 gggaagccat gcgcgataag cgtcgcgttc atcgcattgc ccagatccgc cagcgtatcc
    15541 agcagcgtcc cgtcgaggtc gaagatgacg gcgcgatagc ccatcagccg aaaatcctcc
    15601 gcacgatgtt catcgcgctc ccgcgatagg gcggatagcg cagcggcatg tcgaagagcc
    15661 gcgtgcgctt catcacgggc ttgaggtgcg agaaggcctc gaagctgtac tgcccgtggt
    15721 aggcgcccat cccgctctcg cccacgccgc cgaagggcag gcggtaattg ctcacgtgga
    15781 tcaccacgtc gttcacgcac actgcgcccg acgaggtctc gcggataacg cgccgctcgg
    15841 ccgcgcggtc gcccgtgaag aggtagagcg ccagcggttt cgacatcgag cggacgatcc
    15901 ccagcgcctc gtcgagcgag ccgacggaga gcaccggcag cagggggccg aagatctcct
    15961 cgcgcatcag gggatcgtcc agcggcacgt tctcgatcac ggtcggagcg atgtagcggt
    16021 cccgctcgtc cgtccggccg ccgatgcgcg gcgcgtgcgg tcccatgagg ccgcgcagcc
    16081 gcgcgaagtg ccgcgtcgtg atgatgcgcg cgtaatccgg cgaggcctgc ggatcgtcgc
    16141 cgtagaaatt tccgatcgcg cggcccagcg catcgacgaa ccgcgtccgc acgcgctcgt
    16201 ccaccagcac ataatccggc gccaggcagg tctgcccggc gttgatgaac ttgccccagg
    16261 cgatcttgcg cgcggccgtc tcgatatccg cgtcggcgag gacgatcgtg gggcttttcc
    16321 ccccgagctc cagcgtgacc ggcgtgaggt gtttggcggc agcctccatg atgatccgtc
    16381 ccaccgccgt cccgccggtg aagaagatgt gatcgaagcg ctcgcgcagg agcgccgtgg
    16441 tcgcgtccac gccgcccgtg accacggcca cgaactcggg atcgaaggtc cggccgatca
    16501 ggtcggcgat caccttcgcc gtcgccggcg cgagctccga gggcttgagg atggcggtat
    16561 tgcccgcagc gatggccccg acgagcggcg cgatggtgag ctgaaaggga taattccacg
    16621 gcccgatgat gagcgccgcg ccgtagggct cgggcaccac aaagttctcg gacggccgca
    16681 ggatggacgg cgcgcccgcc cgccgcggct tcgcccatcg cccgaggtgt ttcatggtaa
    16741 aatcgatctc atgatacaaa aagacgatct ccgccaggta ggcctcgtag gccggcttgc
    16801 gcagatccgc cgcgagcgcc gcgaggatct cccgctcccc cgcgacgatg gcccgccgga
    16861 gcgccgtgag ctgccgcagg cgcgcatcca cgtcccgcgt gacgccgccg tcgaaaaacg
    16921 cgcgctgacc ttcgagaagc cgtgcgacgg ccgatttcgt ttcctttttc gatcgtatcg
    16981 ttcgctttcg ctgggtcatg aatgcctcca catcaattca tgcacgtact gtccgaccat
    17041 ggtagtgtac tgcgcaccga gtgtttttca aaattattgc atcatcggct acggcgcggg
    17101 cagggtgata acgcgcagcg gcgtatactc cggcccctcc ggtcggagcc gcgacgaaaa
    17161 gagcgccatc cggtcgaacg ccgattcccc gaagagcgtc cgggcattgc cgtcgatgca
    17221 ggcggcaagg gccgccggcg gacggcggcc ccggcgcacc cgcgcgaggg tgagatgcgg
    17281 cgcgaagggg cgtccctccc gcgggaaccc gagccgctcc gcgaggtcct cgatctcccc
    17341 cgccaggcga cgcatcggac cgagatcggc gcggatgccg caccagatcg cgctcgcgtt
    17401 acgcgaactc ggaaaggcgc ccagcccgca cagcgcgtag gcgagcgcgc ccggcggcga
    17461 gatccgatcg agggcggcct ctatcgcgcg ggcgcgcgcg ccgtcgcact cgccgaaaaa
    17521 tttcaacgtg atgtgatagg actgcggcgc gacggtcttg atgattgacg agaacggctc
    17581 caggagcgcg cgggccgggg cgagcgcgtc ggccgccgcc gcatcgacgg tgagcgcggc
    17641 gaagaggcgg gccatggcgc gtcagtggga cggcttttcg tcgatgatgc gcgggaccac
    17701 gacatgcccc ttctcgaagc gcggcgccat ccgctcgagc tcggcgcgtg gaagcgacgg
    17761 gccgggcgcg tcctcgcgga aaacattgcg caggtccacg atgtggtcgg cgggctggat
    17821 cgcgtcggtg tccagctcgt tgatcttctc gacgtagctg aggatgtcgg agagctgttt
    17881 cgaaaactcc tggcgctcgt cctcggtgag ctcgaggcgg gccagctcgg caacgcgctc
    17941 gatctgctcg cggtcaatct tcatcgccct tctccttgga ggcccgttcg ggatcgagaa
    18001 acaccgtccc gtgcgtgcga tattcgtagc gccggtacgc gaaggcaata acttttttcg
    18061 cgccgacccc gcccggcggc gcgagtacga cctccaggag gcacgagcgt atctccaccg
    18121 gcagccactc gaaggcgacg atccgcgcca cggtctcgcc cggagcgatc accggcaggc
    18181 cgtagccgat cgcgtcgcga tcgtagtcga tctttgccgg gcagcgcgcg cccccctcca
    18241 gccgcatggg ctcgacgagc cgcgcgagct gcaccgccga aaacgccttg gatttcccgc
    18301 gcttcttcag ctcatcccgc gcgatcgggg cctgacgcag ggcgccggcg accaatatga
    18361 tgtcgggcac gcgcaggggc gcacgccccg cattgcggat caccacgtga aacagatgat
    18421 agcgcggcat ccgcgacgtg gagtcgcgcg ggcgtttttt ccggaaccgg cggtgcgaaa
    18481 gcagcgtttc ccagcgcacc gcgtccacgt attccacggc gagctcgacg ccggcatcct
    18541 gtatgatccg gcaccgcgcg ccggtcgcgg acgcgccgcc gtccgcggga cgcaccccgt
    18601 gctcgatgcc gtagcggcgc gcgcaccccg cgctcgcgat cacggcgacc accatcagaa
    18661 gacccgccat ccgcatgccg atgagcctta cggggaatcg gaaaaatgca accgcgattt
    18721 tatcgcgccc gcaaaaaatg gctttactat ttttcccggc cgatgcaccg tacgaaccac
    18781 tgctacacag aaatgcggag cgtgccgtgg aagtccggat cggccttttt tccaaaaaca
    18841 acgagctcat cgaaagcttt accgccggca gcgagccctg cgcgacccgg atcttccgca
    18901 acctcgaaac gccccgcaat gaacagtacg acctcctcgc cgtcgacgcc gacctgttcg
    18961 actccagcga cgtcatccag ttccacctgt cgcgggttcg gaagaaattc aagagcacgc
    19021 cggtcctgct catccccagc gtcaagcacc tggacggcat cgacatgagc tggttcttcg
    19081 acgacatcat cctctatccc ttccgcaagg gcgagctcgc cgtgcgcatc aagcgcctgc
    19141 tgtggcagaa caagtcccgc gaggacgacg cggtcatcga actggggtcg gtcaccatca
    19201 accccaagga atacctggtg taccacgaca acgagaaggt ggagctcacc tacaaggaat
    19261 tcgagctcct gcggctgatg gtgcaaaacc gcggcgtggt gttctcgcgc aaggagctcc
    19321 tcgccaatgt ctgggggctc gactacatcg ggggcacgcg cacggtcgat gtccacatcc
    19381 ggcggctgcg cggaaagctg ggcgacgatt tcaactcgat catcgaaacg gtccgcaatg
    19441 tggggtaccg gtgcatccag gagggcgaaa cggccgttcc ggaacaggaa caatcggacg
    19501 atcccgaaga ggccgcggac gcctgagtga aatatgcttg ccattttatc atcgctgtca
    19561 atagtatgct cgaaaaaatc ggccacatgg gcgcacgaga aaaccgatcg ccggcgcccg
    19621 gcgaccccac gtacccggta cgcgacatta ccataagagg tttatcatga accaggaaca
    19681 aaagattgag aaactcgtgg actgcattct gtccgacgac ctgagcgagt atcgcctcct
    19741 cctgaagaac ctgaaacgga agcttccctt cttcaccggc tcgcgcaaca tcgccgccgc
    19801 cctgctcaag cagcacctgg gcgacatctc caacctcgac ctcacgctca acagcctgca
    19861 ccagcgcgcc gaggacggca aggtcctctt cgaggacatc aaggacggca aggcccggct
    19921 gttcatcaac atcggaaaga accagaagat ctcgcccggc gacctcattc gcgagatcgt
    19981 gaagcgctcg gggatcgacg gcaagttcgt cggcaagatc gacatccact ccacctactc
    20041 ctttttcgag atccccgaga agtacgccga gctcgtcctg gtgtcgctgg acaagtcgaa
    20101 gatccgcggc gtgaccatca ccgtcgagcc cgcgaagcgt cggcgcaagg gaaaggacaa
    20161 cgacgagtag cgacgggacg cgcgtggaac ccgtgacggc ttcctgcccg ctgtgccgat
    20221 cgcgccgccg gaagccgctc ccctttactt cattcgtgcg ctgccgcgac tgcggcgccg
    20281 tcgccaacga ggcctaccgg ccgctcgcct attccgacgc ctatttcacc gaggactacc
    20341 gcgcccagta cggccggacc tacgaggagg acttcgacgc catctacgcc tcctcccgca
    20401 gccgtcttgc gttcctgttc gaccgatttc cccgccccga ccggtcgcgc ctgcgcctgc
    20461 tggacatcgg ctgcgcgctc ggctttttcc tccaggcggc gcgcgatgcc ggggtgggcg
    20521 ccctcgaggg gatcgagata tcgccgtatg ccgcgtccat cgcccggggc cggcttggct
    20581 gcacggtcca cgagcgcccc ttcggtcccg gcgccgtctc ggggcggttc gatatcatca
    20641 ccgcgtggta cttcatcgag cattgccccg acccccgcac gaccgtcgaa gcgatttacg
    20701 atctcctcgc gccgggcggc ctgttcgcct tcgcgagccc gtccgccttt ggaccgctgt
    20761 tccggttcga ccgcgcgcgc tggatcgaaa cgcacccggt cgatcaccgg gtcgatttca
    20821 ctccgcacgc ggcgcgccgc ctcctcgcgc agacgggatt tcgcgcaccg cacacccgtc
    20881 ccggggcgat ccaccccgag cgaattatgt ctcctgatgc cttcggatac cgccccgtcc
    20941 gcttctgcta cgccgctttt tcccgcctcg cctcgttctc ggacaccctt gaggtctatg
    21001 cccaaaaacc gcccgtttcc cgccccgtcg cctgaaaacc cggtaacaaa attatattga
    21061 caatggtccc atcgttttta gattggcggt ttgcatccct gcaaatgaaa ccagcaggcg
    21121 gtagcccgca cggcgtcgcc aacggataca acggcgtatt cacaatcatc tttcaggcac
    21181 gaggaagagg tatttaaaat gaaaagaaca ttccaaccaa gcatcaccaa gcgcctccgc
    21241 acccatgggt tcatggcgcg catggaaacc cccggcgggc aggaggtcat caagcggcgg
    21301 cggcggaagg gaagaaagaa attgaccgtt tccgacgagc ggaagggagc gaagtaatcc
    21361 gggttcggtg aagcgcatcg taccactcaa ggggaggaaa acgtttcaag aggtattcaa
    21421 gcggggaaaa cggtacgccg cggagggggt tcggatttcc gtcctgcggc ttacccggga
    21481 ccgcgaacaa caggtacgca ggatataccg ggagttcgac cgcggccgcg atattgtaac
    21541 ggggataacc atcagcagga agtacgggac ggcagtcgag cggaacagga tgaaacggag
    21601 aacacgggcg atattcaggg aacttctgcc gggtatggct cccggtttta tcaccgtcgc
    21661 gcactatgac gcgcggggcg aggcgctgcg ctatgcggtt gtgcgcgatc ggctcgccgc
    21721 gcttctgaaa cgagcgggcg tatacgaaac gcatgaagat taatggacta ttcatcgctc
    21781 ttgcgattgg attaatacaa ctctatcaac tcttgctgtc cccgttgctt ccgccggcgt
    21841 gcaggtttta cccgtcctgc tccgcctacg cgatcgaagc gctccgcaag ttcggccttg
    21901 tcaaggggct cgcgcttgcg gcgtaccgca tcctgcgctg caacccgttg tgcaggggcg
    21961 gatacgatcc ggtcccctga cgaaacccgg ccgtaccgac ctcattgaaa ctggcagtgc
    22021 gctcccggcc ggccgagtgc ccggtcaccg gcggcgacgc gacgtccgaa ctattttata
    22081 aaggatcagg tgcaccacat ggaaaaaaag gacatcttac gcgcggtagt agccattgtt
    22141 ctttctctgg ccgtctgggt cggctggatg tatttcttca aggaccacat gccgcaggaa
    22201 caaaagccgc aacaacctgc cgcgaccgat gccgcgcaca aggccccggg tgacgcatcg
    22261 cggcaggccg cgccgcatgc gaccggcccc gcgacgcccg ccgcacggcc ccacgcggtt
    22321 ctcgcggccg cgccggccgg cgtcgaggcg aaggacatcg agctcaagac gggcgtctac
    22381 tccgttgtgt tcaccacccg cggcgcggcc atcaagagct tcacctacgc cgccaagaag
    22441 gtggagctgg tggtccccaa gagcaagtat aacgccacgg gcctgttcga tttcgcgctg
    22501 catatcagcg aggatgaatt cctgaacgga agcgccctcg attccatgat ctggtcgcat
    22561 gccgtcaagg agaacacggt cgtcttcagc gccacggcga cgatgcaggg ccttccggtg
    22621 cgcatcgaga aggtgtacac cctcatcccc ggcaaacacc acttcacggt tgaaacccgg
    22681 atcgccaacc tcgggcgcac cccgctgccg cttccgaaca agtacctgat catgtcgcct
    22741 tcggacttca tggggcccgc gctcgatttc gagaacacct acaaccaggt caacagcatg
    22801 ttccagatca acggcggctt cgagaagccg aacaaggggg gcggtttctt ctccaagaac
    22861 gaaggcccca ccgccgtcca gaagggcgcg gtgacctggg tcggcgtctc cagccgctat
    22921 tttcttatcg tgatgctcgc caacggattc agcggcacag gcgccattta cgacaaccgc
    22981 gccgatcgcg gcttccgcgc cgggatgtac gtgccggtcg attccatcca gcccgggacg
    23041 acgctcgcgc gctcgttcaa ggtgtacgtg ggcgaaaaga acaaggacct cctcgccgag
    23101 gtggacaagt ccatggtgga cgcgacggac atcagccgct ggatcgagcc catccgcgac
    23161 ttccttcttt ggtgccttat gaagatcaac atcctgttcg gtaatttcgg ctggtcgctg
    23221 gtggtcttct cgatcgtcac gaaactcatc ttccttccgc tgacgatgaa gtccaccgag
    23281 tccatgaaga agatgcagga cctcaacccg aaaatccagg agatccgtga gaagttcaag
    23341 gacaagccgg acgtcgtcaa caagaagatc atggagatgt ataagaagga gaaggtgaac
    23401 ccgctctccg gctgcctgcc gcttctcctg cagatgccgt tcttcttcgc cttgtacagc
    23461 gcgctcatca actccgtgga cctgtggcag gcgccgttcg tcctgtggat cacggacctg
    23521 tcgctgccgg acacggtcta cgccatcagc gggttcaaca tcaatatcct gccgatcttc
    23581 atgacggcga cgacctacct gcagcagcgc atgaccacga ccgagggcac cagccagcag
    23641 caaaagatga tgatgttcat gccgctgatc ttcatcgtca tcttctggaa catgccctcg
    23701 gggctggtgc tgtactggac gatgcagaac gtgctgcaga tcgggcacca gctgtacatc
    23761 aacaagcgcg gcgacaaaaa gaaggaatcg ccgtcggcat aggttcgcgc cgggggcata
    23821 ttgatatctg gaggaacgta tgaaagtact ggaactcgaa ggcaaaacgg tcgatgacgc
    23881 cacgaagcgc gccctgaagg aattcggcct caacgaagag gacgtcaaga tcgagatcgt
    23941 ggaccaggga aagagcggca tctttggctt cggcgtatcg cgcccggcca agatccgcgt
    24001 atactacagc gagaacacga tggacgtcgg gggcctggcc tcggaggtcc tcgtgaccat
    24061 cctgtcgaag atggacatcg acggccgcgt caaggacctc aaggagggcg agaacaaggt
    24121 ctatatcgag atcgacagcg cgcagagcgg cctcatcatc ggccgcaagg ggcgcacgct
    24181 cgagtcgctg caattcatcg tgaacctgat cgtcaatcat caaacaaaca cggaaaagaa
    24241 gatcattctg gacatcgaat cgtatcgcgc gaagcgcgag aaggcgctgc gcaagatctc
    24301 gaaggaggtc gcgctcaagg tggtgcgcac cggacggccc tggacgctcg agccgatgaa
    24361 tcccttcgag cgacggctga tccacctcac gctgcaaaac gacacccgcg tgaccacgaa
    24421 aagcgagggc gagggaatct accggaaggt gaaggtgtcg ccgcgcggcc ccaagtgaga
    24481 tgtcgcccga caccatctgc gccccggcct cgggaccgct cgccgctccc atcgccgtta
    24541 tccgcgtaag cggtcccggc gcgcacgggg ccgtcgccgc ggtgttttcc cgcccggacg
    24601 cgctccttcc gcggcgcgcg gtcttcggct caatactcga cggcgaaacg ctcgtggacg
    24661 acgtggtgat ggtctgctac gccgcgcccg cgagcttcac cggcgaggac tcggccgaga
    24721 tcttctgcca cggcaaccat ctcatcgtcc ggcgcatcct gtccctcctg cacgcgcgcg
    24781 gcgtccgcct cgccgaaccc ggcgagttca gccgccgcgc cttcgagaac ggcaagatcg
    24841 acctcaccgc ggcggaggcc atcaaccaca tcatcaccgc gcgcagcgac tgggagatcg
    24901 aggcggcgct cgcgcaggcc cacggctcgc tgcgcgacgc ggtccggcgg ctccgggagg
    24961 acctggtcgc ctaccgcgcc gacatcgagt gcgcgatcga cttcgccgag gaggaggtcc
    25021 cgcagctctc gcgcgaggag tcgctcgagc gcataacgtc gatcgccgcc gggatcgacg
    25081 cgatccgccg ccgctgcgcg accggcgagc gcctcgcgcg cgggatcgac gcgccgctga
    25141 tcggccggcc caacgtgggg aaatcaagca tcctcaactg cgtcatcaac gccgaacgag
    25201 cgctggtgtc gcacattccc ggcaccacgc gcgacctcat ccgcgagacg gtgcagttcg
    25261 cgggcgttcc ggtgaacctg atcgacacgg cggggatcgg cgcgccgggc tgcgagctcg
    25321 aacggatggg catcgaattg agccagcgga agatccgcga ggcgggcatc gtcatcatgg
    25381 tgttggacgc cgtcgacggg ctgaccgacg acgatcgaac gatcctcgac atggtcgcgg
    25441 accggccaac ggtctatctg gcgaacaagg ccgatctcgc cgcaacggac gatatcgccg
    25501 cgatcgggcg ctcgctggga cggccggtca ttcccttttc cgccgtgcgc gcaacgggtt
    25561 tggacgcgct cgaagcggcg atcggacgcc tggtgcgcga ctcgatcgtc gagcccgaac
    25621 gcggcttcct cgcggatcac cgcatcatgg cgcttctcga cgaggcgatc gggaccgccc
    25681 aggccgtgcg cacgctcgtg gcggagggcc gcccgcccga gatcgtcgcc ttcgagctgc
    25741 agacgctcgc cgataccctc ggcgcggtga cgggcgagat cacccccgag gacgtgctca
    25801 cctcggtctt cagtcggttc tgcatcggca agtaggccgc gggccgctac ccgcccagcc
    25861 ccttttccac cagaagctcg aaacggtttt tctcagcaaa ggaaaacgag gtgaacatgc
    25921 ggtacatggc gacgccgatg ttttcgatgg tgatgtccgt cgcgtagtag ccatacccgg
    25981 gcacggtgtc catgagcacg gcgatctccc gcgccaccga cgggatgtcg gcgcggccgt
    26041 aggtgtcgga aaggccgccg tcgggcaagg taaaccggta gcccgtttcg ccgttgtcca
    26101 agagcgagag cgagaggcgg agcgggacca cgccccgggc ggctcccgcc acggcgtcgt
    26161 acacctccgg gcccacctcg gtgaccgtgg cggcgttgcg cgcgccgcgc gtcaattcat
    26221 agagccgcac ccgcttctcg gccgttgcaa gcggcgagtg cggattgtgc gagacgacat
    26281 gcgccgcggc gttgtcggcg aaggcatgga gccgcgcgag gtagagctgg aaccgatcgg
    26341 cccccttgcg atagaacgac agcgacccgc actcgtcgat aatgaagaaa tagcaatact
    26401 tccgctcccg ctggaagaag atgttgatcg cccccggcgc ccgacgctcg acgatggccc
    26461 gcaggtagtt gagctccggg tttcgttcgt cgaccgttaa cgtcgcgttc atccccgaca
    26521 ggttgatgag cgaatagagc atcttcacct cggtatcgaa cacgcggcac tcgacggcgt
    26581 ctcccccctg gtgccggcgg caggaataga gcacgaaatt gtttcccagc atggtcagga
    26641 acttgcgccc ggagcgctcg ccctccgcga gaaacacgcc gtacacggtg cgaaacagcg
    26701 attggtacgc cttgaacgcg ccgcccgact tgtagggata cgaggaggtg agccacaccg
    26761 acgcgtcgaa gctctccttg tgggccagcg cgccgttgag gatgcgctcg agcacggcgg
    26821 ccaggtcgat ctcccgcgtg aagcgctcga agaccgtttc gccccagctg ttgtgataca
    26881 aatagtaaaa ttcctcgaac ccgcgtgtgt atttcgagtc gagattgaag atgatgaagt
    26941 tgaggatcgg gaacggatcg accagaaaat agcggttgcg gatgtgcagc ttcttgattg
    27001 agaagtgcag cgtgaggtcg ttgaggagct cgcgcaggaa gttgttttcc accagttgat
    27061 agcgcgactt gatctccagc cgcgtggtgt ccttgagata gagacggttg atgctgatcc
    27121 aggcgagcat cccgatgagg ctgggctcct tgtgcataat gagcattgtg gaggagacat
    27181 tgtacccgat aaatcccttg gtgagcagcc aggcgatgcg cccgccggcc tcgctcaccg
    27241 actccaccgt gagctgcttt tcgatggggt tgtccttgaa tatcaccgcg gggcggatct
    27301 tgttcgcgga gggcgcgagg ctcgactgga tcttacgcgc gaagaatttg atctcggcgt
    27361 cggtgaactt ctgcgcgatg gatgcgcgcc cgacgctggc ggtgatccgc ttgtagctct
    27421 tgagcaccag cttggtgatg tcgttccaga aggtgttcac cgcggtgatg tcccagccgc
    27481 ggaagttgtc gatctcccgc aggcggtcct cgtcccagcg ccactctttc accatcgcgt
    27541 tcatgatggc gatcttctcg tcgatcgacg aaccgcgctg gagggccacc ttcccgccgc
    27601 ccgccgtgac cgttcgaacc atctgcccct gctgcagggt cacgtactgg gagaggttcg
    27661 gctccacctt gaggtagaag caggtcttga gtatctcgtc cgccgtcgga tcctcgcgca
    27721 tgatattgcg cgaaaaatag tcgtacacca ggcggaacat gaggatgtag ggatcgacct
    27781 gctcggtctc cagcttcccc tcgtggacgt tccgcttcac gatggtgcac aggaagtcgc
    27841 gttccccggc cgcgtcgagg agatactttt cgatgaggcc gatcttgatg atggacttga
    27901 aggggttccc cagcgatttc aggatctgga agagcccggc cccgatgaaa tcgtcggcgt
    27961 tgatgacgtg aaggttcccc aggtcgatgt actcccgcgc gaactcctcc ttcgggacgg
    28021 cccgcatcgc ggcggcgtat cgcgcgtcgt cggtgccggc gggcaccacc caccagaagg
    28081 ggatctttcc gttcagcagg atggagctcc ggtagagctc gtccttgagc agtttcccga
    28141 gcgccttgcc ggtgatgtcc acctcgtcgg cgtcgaagat gtccaccttc acgttctcgg
    28201 tgtcgttcac gtagaagtgc gtctcgatgt tgtagttctc ggtgatccag tgctcgatca
    28261 gccggagctt cgagcgcatc gcgtcgagct cgcgcttctc cagcgcgctc tcatcgaggc
    28321 agatccagaa gtcgatgtcg gaatcctcgg tgtaggcgat gctccccgtg ctcccgatga
    28381 tcgcgaagaa ctcgatgagc tgccgccgcg ggcgcgcatc gcccagtccc gcgccgggaa
    28441 acagcgaggc cagcaggttg atcgcgcgcg gcgccggccg gtagccgtag atgccgacgg
    28501 gatcgtactc gggcacaaac ccgggcagct gcgggtggtt gccggtgatg agcaccggga
    28561 tgatgtcgat gaggtccgag aggttcttgc tggacagcga ggcgagaaac ttccgaagct
    28621 tgagcgcgtt aaatccctgg aagcgctcct tgttctgcgc gatgcgtgcg atgatgtcgg
    28681 ccatggcgcg ggcgggctcc ggtatcaggc cgacggcgcg acggcgccgg cgcccttgtc
    28741 gaacaggtgg gcgatatagc tgttgtattc cttgagcatg tccacgacga tgggccgctt
    28801 gagtttgcgc gattcgcggt cggcgatcac ccggatgacc gggttgagcg gatacaggcg
    28861 gttcatgccc tcgatcacgc cgatctcgcc cgagttgagc cgcaccacgt cgccccggtc
    28921 gaagaagaac tccccgttgt tgaggatgaa gcccatcgac cggatgaaat cgcgcaggtc
    28981 attgggcagg aagcggtatt tcttcttgta cggcgcgtag atcgagttga gaagaatgcg
    29041 cagggcctcc cgcggcgcat agggccggcc gtacgggcgc tcggtggtga gggcgctgta
    29101 cacgtcgcag atcgtaagga gccgtatctc gcgggggacg tttttctggt agtgctccag
    29161 gtccatgccg aaggggtacc cgttcgcgtc gggactttcg tgatgcaaca ggatgatccg
    29221 cttctcgatg tcgtgcatcg aggggttccc ctcgacgaac ttgtatccga catgcgggtg
    29281 ctccttcatc cggtcgaact cctcgtcgga gagcttgccc gccttgagga cgatcgcgtc
    29341 gctgagcgac gtcttgccga tatcgtgaaa caggcccccg acgcccatcc cgatccggat
    29401 atctttggag agctccaaac gcgtccccag gatcgccgcg agggtggaca cgttcgacga
    29461 gtgcaccagc gtgtattcgt ccatgctctt cagcttggcg acgggagaga acgcggcgtt
    29521 cttgtttttc tcatacccgt ccagaatttt ttccacaacg ccgcgcgact tggcaagcat
    29581 ggccgtgttg atggtgcgtc cgggatcgac attgaagctt tcacggacgt attcgaggat
    29641 gtcccgggcc tggtcgatgc tctcgcgctg gagcgtctcg cttacggcat tggtcgccag
    29701 gtcgatcccg ccggcggatt ttgccgcatc gtgcttttcc ggctcggtat aatacaggtg
    29761 ctggacaccc ttccccagca ggtggttgat gagggcctga tccagcgcgg tatacgcctc
    29821 aatgacgacg gtgccgtatt cgtccttgag gtcgaatgaa ttgatggtgc ccgctttcat
    29881 gaaccggaga tcgatccggt tcgttcgggt ccccttgtcc tgtccgtctg ccgtgttcat
    29941 tccgcaataa tccgctacgt tcgcttttat gggagtgtac acccagcata tcgcaccggg
    30001 caactactaa tcaaaaaaaa attctttccc ccgtcccgcc gccatcgacg gtcaataata
    30061 cttgacacaa ccgcacacga tgtctatcga tgttacaaat tttgggccgt ttgaaccgca
    30121 aggaggatca tgtgagaaca atacttttcg ccatcggagc gctcgccgtg gccggcatcg
    30181 tcgccgcatc gcccctcgcc gcttccggaa aatggaaggt cgtcaagcag agcgatggca
    30241 ttaccatctc gacccgcccc gtcgagggat cgccggtgga tgagttccgc ggcgtgacca
    30301 ccatcaacgc ccgcatcgag gtcatcgggg aagtgcttcg ggacgtcccg gcgaacgtcg
    30361 aatggatggc ggactgcaag gaggcgcgcg tcatcagccg gaaggacaag gacaatctcg
    30421 tcatctattt cgtgcagggc gcgccgggcc cggtgtccaa ccgcgacttt atcgtccggg
    30481 tgaacacgac gaagaactac gacaagggcg tggtcatcgt caacctcaag gcgatcgccg
    30541 aaccgctggt cccggtcaac cccaagtaca tccgcatcac cgatctcacc ggccagtata
    30601 tacttcagta tatcgaccgc gagcggacga aggtcatcta tacggtgcgc gcaaatcccg
    30661 gcggctcgct tcccacctgg atcgcaaact atgcgagcaa ggacacgccc ttcaaaacgc
    30721 tcgcggggct tcgtcggcaa tccgccaagg cgaaatatgt cgagctggga aacaattccg
    30781 aagataagaa aaagatcgag gaacgcctga aaaagggcgg cctgaagtaa gcgatgaaaa
    30841 cccggcgtca ttggcgccgg gttttcattt tgtattgtgg ttgacatccg gcgtggcgcg
    30901 ctgttcagtg cgatgaaatc ggcttcacca acggtggctg gccatgttga actctgacgt
    30961 tctggtgctc aattcgggat tcataccgat acggatcaca tcggtcagag atgctgtctg
    31021 cctcatcgcc gcccatcggg cggtccccgt catcgaggac gacgagtatg tccgctcgcc
    31081 atccatatca atccgcattc cgtcggtcat ttcggtcctg ggatacaaca gctttccgca
    31141 gcgtcgggtg gtcttttcca agctcaatgt catttaccgc gacgacatgc tctgccagta
    31201 ctgcgggtcg cgcttcaaca tgaaagacct tacggtcgac cacgtcatcc cacgcagccg
    31261 ctgggaatcc gtcaccgggc gcgaccaagg cgacggcttc tcgacatggc acaatctggt
    31321 atgcgcctgc cgctggtgca acaaccgcaa gggcagccgc ctgctccatg aactcgggtg
    31381 ggccctcctg cgcgagccct tcgagcccga gtacatgccc tacatcttcg tgtccttcga
    31441 acgcgccaag caaaagggct ggctcccgtt ctgcaacttc aacgtgcgga ttatcggagt
    31501 cgtagagtag gcgattgatc tcgcagccgc ctaccgttat gtctcataac ccaattgatg
    31561 gaggtattcc atgaaaaagc tgtgcttgtt cctattagta gccgtattcg caacaactac
    31621 gctgatggcc gctgccccac ggaaagagaa tcggggccaa tgccgaaacc ggattctaga
    31681 gctttctaaa ttagagtacg gtaaatgtga caaactcccg agcagaagca aggaggagaa
    31741 ggaaaaaaga agggaatgcc aaaaaaatgc cggtgcatgg tcaaaagcgg aaatggccaa
    31801 atgcgataag ttataaccat acgcagagaa ccggtttcgg gaagaccatt agtttacatc
    31861 aaaggcattt cggaaccggt tctatcatag ccccctcttc tcaatcccca cctcccttca
    31921 aaaagtgcgt gacagcgggc gaacatttag cggtcaacgc gtacgtcggc tgactccagc
    31981 agggactcgg tggtgttctc cggattgatc cttgtgaaca tgatgtcggc ccaaaacttc
    32041 gggtattgct ttgcgagttc gcgaaatatc gccaatcgga tttcgtccaa ccgacggatg
    32101 gtcgtgtgct cttcccgaag cgaggaatat aaaaagaaat actgcgttcg gccgctctta
    32161 agcgcccaga cctgcacgtc aatcatgccg tagtgttcga tgacgtcccg cagcagcccc
    32221 ttcacctcgt cctcgatgct gttctccggg gaaaccagga gcagccgccg gaactcgacc
    32281 aggaccacct tcaggggaac gggaaacgac actacgatga gcgcaatcac gatgacggga
    32341 tcgacatacg gcagaatgcc cgtatacccg agacgcatga gcaccatggc gacgacgatc
    32401 cacccaaaaa tccagaagcc tggccacggc gtgttgaact tctgcatcga gccccacatc
    32461 attccgatga gtgcaaaggg aagggcaaag atcgagtgtt cgatcttcac catctccaga
    32521 aaaaccgtga atgcctgcca gccacgggca gccgttgcca tcgcaaaagt gtactggcat
    32581 tttcagattc cattgggtaa gttccgggaa tgtctgaatt cgatatcaaa gcttttatct
    32641 cagccaacct agaacggaac gcaggaatgt acgtctagga cttcgaagcc ctgccagatg
    32701 acgccctcgg ccgtagtcca agtggagttg ctcgatc
//
LOCUS       DAORHF010000009        32193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_787_length_32193_cov_5.879208, whole genome shotgun sequence.
ACCESSION   DAORHF010000009 DAORHF010000000
VERSION     DAORHF010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 32193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32193
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_32193_cov_5.879208"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKJ16_01660"
     CDS             complement(<1..271)
                     /locus_tag="PKJ16_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HNV45716.1"
                     /translation="MSEDTFIDIVVNKKARFEYEIVDTFEAGLVLKGTEVKSVRLKQA
                     SIQESYARIAKGEVFLVGMNITPYAHGNRFNHDPLRERKLLLHRQE"
     gene            complement(473..2647)
                     /gene="ccsA"
                     /locus_tag="PKJ16_01665"
     CDS             complement(473..2647)
                     /gene="ccsA"
                     /locus_tag="PKJ16_01665"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV45717.1"
                     /translation="MNAGHLLTIAALAAAAISLVFRLRDAMGAAGSARPARIAFTASG
                     IAMAAAMGLLAAAFVTENYSYAYVYNYSSRDLPLAYKIAGLWAGQQGTFLLWAFVLYA
                     IGTVLDRARAGQNNIVVAVLTVPMLFILLIIAMPDYSPFRFVWDAMPDRIAPGAVPAD
                     GAGLNPLLQNFWMISHPPVLFIGYAAATVPFAYAIAALVRNDFTDWAARAYPWALFTM
                     VSLGIGIFLGGYWAYTVLGWGGYWGWDPVENSSLVPWLSTVALVHGLIVHRRKRALVK
                     SNIALALVTFVLVFYSTFLTRSGVLSKFSVHSFGDLGLSAHLLVFIAATAAGGIALFL
                     WRRSSMTAEPLGESLLGLDNLIACGVVVLCGYAAFILIGTSMPILSRIVVPEGSSVKA
                     EFYNNLSIPAGLLLLALIIAASRAAWPRAVGRWAAAILGCAAIGIGIALNVGSTTNAF
                     AYLFAAFGSLAALQALHAIVRAPSRRAIAARIAHCGVGVLVVGFVTSNLHSTTAQREL
                     TQGATTAVGPLEIVFDGITGRDTTALAFTVRDGGRERAIRAPYFVDQRSGALYREPFV
                     IRGLVRDVYIAPAEYRPDGGGGTRLHLEKGKPQAADGTAFEFLGFDTKLMNMMAGAGT
                     ILAEVRVRGAGGTHTVSPGLIIENGKAVGNYDAALPGTPAKKIALLSMNVSAKEIHLL
                     VTEGAPPSVLVDISFKRLIWLVWLGTVAIAAGGIVGLFRSRE"
     gene            complement(2644..3021)
                     /locus_tag="PKJ16_01670"
     CDS             complement(2644..3021)
                     /locus_tag="PKJ16_01670"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HNV45718.1"
                     /translation="MKINNKILIGMGVALCAGIAFYSLRGAITPYVSFAEARSGRASV
                     QIIGKLDTRSPVRHENGAMLFTIADDRGERMDIAYRGPKPLNFEHAEQAVVMGSYDPA
                     ARRFAAEKLLVKCPSKYTAEQRP"
     gene            complement(3011..3328)
                     /locus_tag="PKJ16_01675"
     CDS             complement(3011..3328)
                     /locus_tag="PKJ16_01675"
                     /inference="COORDINATES: protein motif:HMM:NF040113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HNV45719.1"
                     /translation="MKSLIFVTVMLMAPAALIIPATLFFDTAAPHGYTHRAAPCDAAH
                     ASTRDSAAEKAVPTQPPREGTSATGERIPLVVVSIIVWIGLGAYLYRIDRKISRIEQG
                     HED"
     gene            complement(3325..4005)
                     /gene="ccsA"
                     /locus_tag="PKJ16_01680"
     CDS             complement(3325..4005)
                     /gene="ccsA"
                     /locus_tag="PKJ16_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV45720.1"
                     /translation="MTHARLITYALRSIYLLMPLSIALSLLWAPPAAVLGESSRILYL
                     HVPIAWVSALAFAVAGAFAIAFLADRTGRVRLLDEKAHHSAELGFLFAVLATATGAVW
                     ARAAWGSYWNWDPREMSITFLLLIYVAYFSLRASLAGSPARGRICASYLIIAMAAAPF
                     FIFVMPRLYPSLHPDPVINPDRKVHLDGRMQITLAIAVCSFTLLYAYLMHLRARIASI
                     AHRREGSS"
     gene            complement(4406..5062)
                     /locus_tag="PKJ16_01685"
     CDS             complement(4406..5062)
                     /locus_tag="PKJ16_01685"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HNV45721.1"
                     /translation="MGELAKNIRKDLTVEFRNRYALSVALAFAAVATIAASIALAGTV
                     PAPRVQSALLWIVVFFAAMNGLAHAFVREEEQGTALFLRLHATPHTVLTAKLAFNIAL
                     LAAIEAVAIPLFVVFLQLEVRAPHSFVAIAAAGCLCLACAVTVLGAMVARAGGRGALF
                     TVIAFPVVIPALWVLIGATARSLERPGPFDSGSVLFLLAFSGAMAGVSYLLFEHIWEE
                     "
     gene            complement(5055..5669)
                     /locus_tag="PKJ16_01690"
     CDS             complement(5055..5669)
                     /locus_tag="PKJ16_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV45722.1"
                     /translation="MRNTESAIAAHAVSARFGRVALFEGLTLTVAPGESLAIVGPNGS
                     GKSTLLEILAGLRRPTAGRVLFTAGGDDDARTDRSRIGFMGPRVEPYPDLTGMENLLF
                     AARGGSSRERAAALMERFALRPHRDKLTRHYSSGMRQRLKLAIALVNDPPALFLDEPG
                     AMLDEAGRALLRAEIDARRAERIIVMATNDADEAALCSRRTALG"
     gene            complement(5679..8261)
                     /locus_tag="PKJ16_01695"
     CDS             complement(5679..8261)
                     /locus_tag="PKJ16_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV45723.1"
                     /translation="MPATILHHTARIATLVCALIAAALLSPADARAAETLVLTDAGSR
                     YPLGTRCDLLEDRAGTLTVADVAGPSLASSFRPLDAAVPNFGYTRATYWFRFSLHNTA
                     DPRREWLLVLPYALIDDVRLYTPDGARGFTEHASGRLNPASKSGNFNRHFTLPLPPLA
                     RPTVCYVRVASKDSLPVPLEVREARQYRDHITRDHHVFGLYYGIIVVMVLFNLIIFIV
                     TRDRNYLLYSGTLVSLHGLTQLGINGFSAHYFGDSIWWSRTSISFFVSVGAGMAGFFC
                     ISFLRLRELFRPLYYSILACSVLLMTSAVLSLVVDYYYSIQLSVILCMIGSPQFLLAG
                     FVQIRRGYREARFYLLAWTMLVLCGCLYGLKVFALAPHTPLTEYAWQVGLAVETIILS
                     LALGDRINLMRQEQMRAQNDLLQKEREARETQERFNESLEKLVEERTNELNSALDELK
                     QKDLIIQRELDIAMDIQQNILPPMPLSYNGIRMVAFYRAMEKIGGDFYDIYPMKGGHL
                     CVLMADASGHGIPAAFVTAMAKITFSEATAQLQFPRDILRQVNEQMIRIIKTQEYLTA
                     FLVIISPSYEVFFSNASHQKAFVARARTNELDTWDTNGLFVGAVIEANQMYEEKQDYL
                     DFGDRLILYTDGLVEARNSHGEEYGQARLRELFIETFPLPLDAAKNAIHEHWRTFIGE
                     TPVRDDVTFLLVEIDPAYRELLEYKNRGLEHLYHNRVNDAIAELKQALKIDNRDAKVH
                     HLLGKCFFKERAYDEAITHLSYYTGTNAEDADAFNMLAAAHYNSKRYEDALAAARQAC
                     ALRPNFKNALSTCALALMKIGKAEESKAVWEQILAIDPANSIAKAELLIIQGQIDGGE
                     TPAG"
     gene            8444..10555
                     /locus_tag="PKJ16_01700"
     CDS             8444..10555
                     /locus_tag="PKJ16_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNV45724.1"
                     /translation="MSHRRTPLLERLLPVSGGAAVMLVLFGYGLINSFSMSYMGNTSE
                     RVAQFIISGFLGHIALFMGKILLAYLAIGAALGIGAWAVYRAADELFDLDWSALQRFV
                     ANAAAGVLVVHLFLFRDIIAYPQVYINNFHAKNALNRFVFDALTEHLSPALFSVAIYF
                     SIALAVAAGGFLLYRFDPRIARWGGALSGAAVIITLLAVNLDLSRPGDARRPNMLILL
                     TDALRADRLSALGYARPTTPHLDLLVKDGAAFENAFIEAPRTFPSMVSYLTGQYASTH
                     GIRHMFPAARDLKKDFVALPRILRESGYRTAVVADYAGDVFSRIDLGFEAVDTPFFNF
                     NAVIEQAVLDNHVFLLPFLTGRAGLFLFPVLRDSATFCPPRLVGGRVIEQIRKSHGRP
                     FFITAFFSATHFPYAPPHPYYAMFRAKGYRGPYRYLKQRELSLDGAARAEISAADIAQ
                     VRALYDAGVRAFDDAAGDILRYLSRKGALDDTIVVVLSDHGENLYEHEFGMGHGEHFR
                     GYHTIRVPLVMRHPRLVPRGARVRDLAHQVDLAPTLLALAGLPAPDSMEGASLVPALR
                     GGRLARRYAYGETGIWFDNDRSGVHFFQERRIQYPDITAISEVDRSFSNHIVVGDAYR
                     DIVNIAKHRYIFDGRYKLIYMPRREGVAYELYDAHRDPDDRTDLARRDPATRERLARE
                     LFRWMTRNGDVVVKNGFVVPR"
     gene            10621..11847
                     /locus_tag="PKJ16_01705"
     CDS             10621..11847
                     /locus_tag="PKJ16_01705"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV45725.1"
                     /translation="MKLPRSTMEALAPRIITRYIIREQVPALVIGLLFFTFIMMLNRI
                     FTIADLIINRRVDGWLVFKLFLYMLPITISFTVPMSVLISAVMALSRLSADSEITALR
                     ACGVSVYRIIKPVLVSGFVVFLMVLAFNETLFVYSNRTYNQLFVQILRSSPTSVLEPK
                     MFTTVGDKTIWVGQIEREVERLGDIMIYNRNPAGGWDIIRAQEGLWKQNADGSKSLIM
                     YRGKIFSSDFDKATFSYLDFSNGSAEVLMTESKIDYTEDRDVINPHEHDSAGLLARLR
                     AHPKSYRNDRNVALNWIEFFKKHAVPFSCFVFSVIGAPIGIFSTRSGRGVGFGVSIFI
                     FFAYYVFFVGGQSMAVRGDVHPALGVWFTNFLFMLIGVLLIVFKERLDTANIDIHALA
                     GRWQALVGRFRKRRET"
     gene            11879..12757
                     /locus_tag="PKJ16_01710"
     CDS             11879..12757
                     /locus_tag="PKJ16_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45726.1"
                     /translation="MREVSIWYLTDNESGASIARGIGELGLQVRTITDGDFAKANIIA
                     DIINLFVIDLKGTDLESVIDIIKRDARINGFLKFVVLGKRQAKKVHQVAVNLLHVEYL
                     TRPLGLREFTLLLEKTIVVEKYREIMKLISRDAQERIETYESLVGIGRRDAFDVSAHR
                     EVFERIIENERQLMAEQKKLNETLREVSLLRQAEMFDVRNRVKADEMLSELRRHELMD
                     AKNIIRAQEAVIDYSSQELKDAERFIDASTRVQELSRAEAIRLQEELASARRENERLS
                     GEVKRLTRELKRAARG"
     gene            12765..13328
                     /locus_tag="PKJ16_01715"
     CDS             12765..13328
                     /locus_tag="PKJ16_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45727.1"
                     /translation="MRRPIARLGLALLLVALCAGDARAWLFDAGLEARIGEIRARFRE
                     RRLMELYVCCVDPRYKLRIEKVVQRMKDDRRTGHAIARRLGFANHAELAAARDAAVVE
                     RLFALIVHPPAERVRIDNPDYQRLGLLLSLLFSCFETGMDITGRDVYRDRARVFFSGG
                     RLKMVAYFVFLDGAWYVTEKSLLGNDF"
     gene            13346..13618
                     /locus_tag="PKJ16_01720"
     CDS             13346..13618
                     /locus_tag="PKJ16_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45728.1"
                     /translation="MEWEKVLANAVQDGRIRELHLRKIPRLKTCANWREVEPIGWIDY
                     KMRFAHYKGGIVKLNSKIYFVTEQTLNALSEFISIKFKTTLAVIKD"
     gene            complement(13615..15477)
                     /locus_tag="PKJ16_01725"
     CDS             complement(13615..15477)
                     /locus_tag="PKJ16_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNV45729.1"
                     /translation="MSRITEAVGRLSPEDLKRIAGLLGCAAGSSESASAALAARLLTY
                     DGLAAVLGDLDPREMQVLAAAADDENGATYSEMEKKLRLPADLIEEAAGALAKRLLAH
                     ILKNRQRIHNRMDRLIVFPEVRRLLASRDPAELAAGYGDMLARLATGSVPALPLPGDR
                     ASKKLLAAAAARGGIIPYDTVGSVVSGAAREKTIRDLAEKNIMEIFPVLASPFHLYCA
                     LSPEALAATLGDRSEDPGAGEPLLSNHCRFLLNMLHTYDVILSTGLFLTQQREFRKVD
                     RKRIAEAMAPMTDIDGEPLDPDAAAQLALHCLHRLRCIALERDAARVSLKAIAGDLDS
                     PARLIGKLLRNNEPRDDGGLFGTPLPIPGFAVAREALDLATRYGTRTPESLRALHLAS
                     LVARGKNTLRLADADEQRAAVRSFDESLRFLVLCGAISMSAGRATPTDIGHELAGRAA
                     RDATRGSSAAIYINPDFTLIIPHREIGTETLFHLLTHTELLKEDLMVHARISRTSIVR
                     AHKRGMPPDRFLDALGALARNGIPQNLQFLITEWLGKTIHVRISRPVLVHSNQPTLLD
                     ELSYGKLKDAIVERIAPEYAIIRAQHLDDVVRFAGERDAVIGLFEEEDETVGGD"
     gene            15602..16672
                     /gene="lepB"
                     /locus_tag="PKJ16_01730"
     CDS             15602..16672
                     /gene="lepB"
                     /locus_tag="PKJ16_01730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV45730.1"
                     /translation="MLQHLRYGGTVAERAEFPIITGSGAYKFLFVRFGYLVILIYSIF
                     FLAVAIPFEGTYFIGSFIATLFLVRFVNLCVMFARYRNGRIAVDNGAIAVTDASATVT
                     LPSSDITFLEVNPMGNLVIRQKYDKTAFPLVLLTDEDQSKLLDLFEDMAPKKTALTRK
                     VIDFFDAILVAFILAMHIRQYIVQAYFIPTGSMEDTLQIGDHLLVEKITFGPIIPRMA
                     GMKDKVHLRMLGLRDIKRGDIIIFKPPHEENKDFIKRCVALPGDVLNIKNGAVFVNGK
                     KAVEPYVRGATSYRGFSDQKIEGVVPPGKLVVLGDNRENSYDGRGFGYLPISEVKGKA
                     FILYFNWDQIKNFDFSRLGLIR"
     gene            16686..18536
                     /locus_tag="PKJ16_01735"
     CDS             16686..18536
                     /locus_tag="PKJ16_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HNV45731.1"
                     /translation="MSQGAFNRRVYVIGIIVVGIAFTYVYKMFELHFSDRVSVGAGRR
                     ANVRRGTITDQHGAILAVSIEKHSLFANPEEIADPAEAAAFLAPVLGLPRDFLQARLM
                     RKKRFVWLKRMLEDDVAEAVRRRALKGLHLKREYDRVYPHGTLAATVIGFADREGRGL
                     EGIEYRFDRELAGARRARGIPGVRAEAPPLCDISLTLSRPVQHAAEEALADACRAARA
                     KQGSALVIEARTGRILAMAKYPLFDPNAYAQADDDERRNFSIVDSFEPGSTLKVFAMA
                     ALLEHHPAVLKASYRCEGYVEIADVLINCTRIHGRLDADGVIAHSCNAGMIQAMKPLT
                     REHLHDLLARVGFGRAVGADMPGESEGLLRPVAGWSGLSKYSISLGQEISVTSLQLAA
                     AFCALANDGTYVVPSVIESVTDADGVPVRRFYTRTRGQVIRPDIARRLVSMMRGAVSE
                     GTGARARLAQYQVAGKTGTAQKSSKQGGYAPNKYIVSFAGIAPAERPDLCVLVVIDEP
                     AGEFSGGDLAAPVFAGIVRRALPLRGVKAALVPAAEPRASVFAPPRLPIAVMPDLRGM
                     SLSEALWVLVELQNRYGATFAITGGGTVHAQEPAANTALSGKERITLFLK"
     gene            18565..18705
                     /locus_tag="PKJ16_01740"
     CDS             18565..18705
                     /locus_tag="PKJ16_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45732.1"
                     /translation="MKSDHIVHVAQGGRATGLSLDELEEGQDDADEGRQEPDQEGQPF
                     FY"
     gene            complement(18699..18923)
                     /locus_tag="PKJ16_01745"
     CDS             complement(18699..18923)
                     /locus_tag="PKJ16_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45733.1"
                     /translation="MQTRTKKAAPISFNDYKHIKDEIIEISEQRFENILTRRLSEFKN
                     RSWRSRTFDSTQSTLGSIRWNQNSSPGSSQ"
     gene            19028..20881
                     /locus_tag="PKJ16_01750"
     CDS             19028..20881
                     /locus_tag="PKJ16_01750"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV45734.1"
                     /translation="MSILERSDPALAARIAALGDDPAIVVHESRDGNRVPELRAEGKT
                     VLLHSRFDPVKEAGRLLADFDPQEYDLVIVFGVAFAYHLEALLPRLRPDATLLALERR
                     AGTLRAAMASRDLGALLADRRVRLAVDPDEEALVSLLSSASSYRITFITHRGSFQADP
                     AYYNNLVRLVKASLSRKEVNIATLARFEKSWSANIARNINAIVAGPGAGAFFGRFAGV
                     PAIVAAAGPSLSMSMDFIRANRDRAVIVAVDTSYRILRKHGVEPHFCLSVDAQLVNAR
                     YFECDTRTRAVLVADPTVHPAVLRLFRSRAAVAGMAFPMMKWIDEIAGGRGELAYGGS
                     VSTNAYDFARRLGASPIVLVGQDLAFTGGRAHARGSYLEEEVYLRVGRLWTMESFNRR
                     QLTALPKILVKGIRTPSVHTNQKMMIFHGWFSRQNDANLVNATYDGAQIAGVRSVAPE
                     EIAIGEPPFDIAARVDECYGAWRSGDGDAASAKKHLLKKVSAMHDELQALLPVLARAE
                     TLSGELVALVRRGSRDRSRFESIMGRLAEADRAVESTRDLKDMIGFTIQRVIHTITEG
                     YEIDEAEGVAGDDERAALRSQFMYRGLHEGARYNRKLLRTMMTLLAKGGSQ"
     gene            20878..21492
                     /locus_tag="PKJ16_01755"
     CDS             20878..21492
                     /locus_tag="PKJ16_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45735.1"
                     /translation="MNTNVIHAIVHIQNWGESSGVMEALVRSKNVISVFHVMGRASYL
                     VDVNFDSKAELEVWINQVKAIRLASGVPAIIAIRTQKVIEAHKQKRGFDLASYRNLGR
                     MFHFFLNIDVPHHDEETVALLQGSDIVHTVLHVQGEHSFVAEIITERYDAYKSLLKSL
                     KGAGGVNHLETQEVIAVHKYRNQVFDETGNLTYPREDIREMFSL"
     gene            21526..21948
                     /locus_tag="PKJ16_01760"
     CDS             21526..21948
                     /locus_tag="PKJ16_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HNV45736.1"
                     /translation="MNKKPAEPHIDDPVNYAREVVGRDPMATHLGIAVEEAREGYART
                     ALTVRPEHLNAVERAHGATVHAVADQAFAVACNSLGTLAIAVSMKITYVSAARDGERI
                     VAEATPVNIGRKVSVWRIDVRVAGGALVASCEGIAYHK"
     gene            complement(22060..23490)
                     /locus_tag="PKJ16_01765"
     CDS             complement(22060..23490)
                     /locus_tag="PKJ16_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45737.1"
                     /translation="MNTVIDRLLRRYDNAPYLLRSKARFLLYVCFSVMIIMPIGIWYT
                     GFLQLINPLSRNAIILPVLAPSFAIMAITGAVIVFLVRGWFRVAAHLFLISALTCIWA
                     VMLVDAAESVSRLDTISLVFAALTILPIVVLRKKRVIIIYVAANIIILYAFMFHVRSE
                     LHLSVNSFWDFLADNTLALVIASITLYNVYAINQRAIERAENEIAERRRAEEERSALQ
                     QQLFRSQKLESLGMLAGGIAHDFNNMLSVIIGYADMLKIDLAAQPQAREQLERISEAA
                     MRSREMTQRLLAFARRQPLETKPIDLNSLIADFGSMIARLVRTNVTVDVRIGDRALMV
                     RADENQLVQVLLNLVLNAQDAMPDAGDIIIEARTALVDARMAQRRTGCAPGRYHVLQV
                     SDTGPGIAPEIKEKIFDPFFTTKTPGAGTGLGLSIVYGIVAQHGGFVDVSSKPGAGSV
                     FAIYLPADGMEENERRVIDGALSSHG"
     gene            complement(23571..24137)
                     /locus_tag="PKJ16_01770"
     CDS             complement(23571..24137)
                     /locus_tag="PKJ16_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV45738.1"
                     /translation="MEQNKPGGIAFRPWNILVVIVLLGIAVGITKYGVAVPELPARYF
                     HAGGIIAAVLGTMYFLRNLIRHLRSYRWSATRATITASHIHKIDDSDGVQYQPHISYT
                     YTVDATEYASNRIQPSGDWSSSFPSIAHKQVNRYPEGTTVHAYYNPARPEESYLERTG
                     LLPILAGLITFVISAIAFSLALAGIIRL"
     gene            complement(24241..25665)
                     /locus_tag="PKJ16_01775"
     CDS             complement(24241..25665)
                     /locus_tag="PKJ16_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV45739.1"
                     /translation="MDLTLVNLNMLYVKYLDGSLRRQNHLPLGPLYLVSVLTEKGFAV
                     DFRDYQLLEGDAIFTPEAFCDFLADPAPVVGISCMANLMPFVAYAMPFLRERYPDRVF
                     VIGGVGAIDIERELMERVPSIDVIHRGEGERSVPLLMAALRGGGPLADVPNIFYRESG
                     AIRGNPPAERIAELDSLPLPSYDGLDFKRYAGHNMLGSRGCPYQCTFCSIAPIWGWRP
                     HLRSVENIVGEMVHMHREYGVRQFLFQDEFFISSPERTMRFARLLAKEKLDITYKAFA
                     RVDLVDRDSLQAMADTGCVEIRFGIESGSDRILDMIRKDITSQTAVETVALAKRIMRS
                     VDAFFVWGFPFETMKDFSESVFQMIAMRGMGVHILPSLLTYLPQTRLYRDLADRSLLE
                     FCPFLLPEFMICGMERRVSVRVDIDGRYGGLFDFIVANKDIFPGFFQVDVENNVLPKL
                     AMLEEFEFYRTDEDQCCGAHSPEP"
     gene            complement(25708..26457)
                     /locus_tag="PKJ16_01780"
     CDS             complement(25708..26457)
                     /locus_tag="PKJ16_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45740.1"
                     /translation="MDDKLTIVGPEPSERGVVTRFNKVNIEALLLMAKYDPGFRRLLR
                     EDRERALDDSGIAFSDSERAIITSISDTQLDTIIGEFSIPGVTRTSLASWRIAASVVL
                     LVTSAMFANVGCGRTFIDISDQSEHDREGWINNDTFRVISLGAPQKKLEKMNDEKART
                     KAQKDSARAAGILSAQHRMLEKFKGAMLSGAAGGALADDGNSVDAKHWKKVIALVKEK
                     GTVVGETYDKDNNCEIRFELNVPGLKKMILE"
     gene            complement(26569..27033)
                     /locus_tag="PKJ16_01785"
     CDS             complement(26569..27033)
                     /locus_tag="PKJ16_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45741.1"
                     /translation="MSICLSAPANGHSTTWNKGVAIVHGEPKYFDPSKFDIPQMQMTR
                     AYIRNHPKNIVPAAVITWLREHDGSVYAITHDHDKTKGSEISEGSKRYYYTIRDAALY
                     VSADAKTWDRLRVRFLNRKPYVSGGVMVWWRVRLTCRWFEGEYEIFSTAIGT"
     gene            27327..27938
                     /locus_tag="PKJ16_01790"
     CDS             27327..27938
                     /locus_tag="PKJ16_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45742.1"
                     /translation="MSRIAICRRALCALCAILATITLSGAQEKSADRPSTDAPKRERP
                     ASTSARSKKAPESCDAACRPLSGSAYNRCVARCEKSGARRDAAAPEGTRSAMVACVKG
                     CGGLSGGAKTECLRRCRETDASRRPSSSNEAVFKECAERCGTLSGQARYGCMRTCASH
                     RREQGGDRDDAKSVRYRECEGRCGVIEGPMRYKCVQNCVRTSR"
     gene            27962..28342
                     /locus_tag="PKJ16_01795"
     CDS             27962..28342
                     /locus_tag="PKJ16_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45743.1"
                     /translation="MIDGKPRHLPERDEFDERMVSIESAIFDLQISLLQFVANLVASF
                     GDDAGVDIPVRVFSGDDYAIVPAKRIYFDRADELLKIELADGDEIVASSLDIGMLEHV
                     ARQLLFRRTSDRAYRDLAGDDEVH"
     gene            28411..28893
                     /locus_tag="PKJ16_01800"
     CDS             28411..28893
                     /locus_tag="PKJ16_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45744.1"
                     /translation="MKAIRGLIAGCAALALSCGGFIRAEDGKRIARLEQYVFVARKDV
                     SNDQRRLRAGQPIRLFIRAEDKWIKVYGYPHDQNILEADRVLLLYLFPDDFKGSKYDH
                     DFFIWRLADEVVLLADQPVDKKKDAKQDAKKGAPQAAKKDVRSDAIEREPLDPRKKNQ
                     "
     gene            28908..29786
                     /gene="dapA"
                     /locus_tag="PKJ16_01805"
     CDS             28908..29786
                     /gene="dapA"
                     /locus_tag="PKJ16_01805"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HNV45745.1"
                     /translation="MFEGLHTALVTPFKDGGIDYDALERIVELQIAGGVDGILPMGTT
                     GESPTVSYEEHKEFIRRVVKLARGRVKVIAGTGSNATDEAICLSKAAADDGVDGIMLV
                     NPYYNKPPQRGMVAHFAAIAQAVPLPVIIYNIPGRTGINFLPESLTELADRCANVVAV
                     KEATGDIAQMMRTIELLGDRITLLSGDDNLLLPVLSIGGRGVISVLSNAFPAEVKRVI
                     VDYEAGRIDEARANFYRLLPLCRAMFLETNPIPVKAAMELLGHCSSDVRLPLMPLSEE
                     NRRALKRAIDDFGASR"
     gene            29783..30580
                     /gene="dapB"
                     /locus_tag="PKJ16_01810"
     CDS             29783..30580
                     /gene="dapB"
                     /locus_tag="PKJ16_01810"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HNV45746.1"
                     /translation="MSRIGINGAAGRMGRAVMGMLLARGHSIAFAFDAPSTPVIGTDA
                     GALVNRGPMGVTVSAIDGSALAGADGIIDFSSPAASELALAAARAHSVPIVVGTTGLS
                     DAGMRLIDDAARDIPVLFSPNMAMGVNLLFKLAETAARVLGPGFDVEIVEAHHRMKKD
                     APSGTAKRLVEVIKNASPALANAPEISGREGITGERSDAEIGVLAMRGGSIVGDHTVH
                     FIGMEERIELTHRAVSRDVLARGAVVAMEFLIGKKPGRYGMYDVLGL"
     gene            30580..31560
                     /locus_tag="PKJ16_01815"
     CDS             30580..31560
                     /locus_tag="PKJ16_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV45747.1"
                     /translation="MDRDKAEQRPDGDAPAEDLKKFDENPYQPDRVKPEDVVRIFYER
                     DTPLVPVVSNRNMLLGVLLKEDVVAELSDIERVKHQKIDEFVSKLSRKMTFDELLPAV
                     ANNRSFATINLFGEVTGEWTRLDLLAASEKGGGSATAIRREAEKQREEQALEWMIYLI
                     LEHLPRALYAVNDRGKTIFFNSHFEDLVVANTGAEIDVDAVEKAFADTDANEFFYRPE
                     GDEIFFYNKELDIHYEKVPMTSGERTVGFLIYCSRSKTDHARPATPHVETAGMSLNQM
                     LDAFVRKVLVDAVKTHGYNLETIAKELGISRPALLKKIQKYRISMQGGHS"
     gene            31992..>32193
                     /locus_tag="PKJ16_01820"
     CDS             31992..>32193
                     /locus_tag="PKJ16_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45748.1"
                     /translation="MTRQTSNAAANVKNGNGDETRRRGPAENKSGDANGANGTSGNGK
                     KPQASAERLDLAELKSKTINDLV"
BASE COUNT         5749 a        10044 c        10707 g         5693 t
ORIGIN      
        1 tctcctgccg gtggaggagg agcttgcgct cccgcagggg gtcgtggttg aaacggttgc
       61 cgtgcgcgta gggggtgatg ttcatcccca cgaggaacac ctcgcccttc gcgatgcgcg
      121 catacgactc ctggatgctc gcctgtttca gccggaccga tttcacctcg gtgcccttga
      181 gcaccaagcc cgcttcgaag gtgtccacga tctcgtattc gaagcgcgct ttcttgttaa
      241 cgacgatgtc gatgaaggtg tcttcgctca tggcgtttgt cgcttagtcg acgtactcgt
      301 gattgccgaa ccggtcattg cgaggagcgg ccgcgacgaa gcaatctgat tcggtacggg
      361 gaaaagtcaa ttgaaatatg gaatacgcat tgccccgctc cagtgccgga ttgcttcgct
      421 tccctcgcaa tgatccagag ggtatctcct cggttgcgct gaatgaagcg cgtcattcgc
      481 gtgatcgaaa caatcccacg atcccgccgg cggcgatggc gaccgttccc agccacacca
      541 accatatcag tcgcttgaag gagatgtcaa ccaaaactga agggggcgcg ccctccgtga
      601 ccagcaggtg gatctctttc gcgcttacgt tcatggacag aagcgcgatc ttcttcgcgg
      661 gcgtaccggg cagggcggcg tcgtagttcc ccaccgcctt tccgttttcg atgatgaggc
      721 ccggcgacac cgtatgcgtt ccgcccgcgc cgcgcacgcg cacctccgcg aggatggtgc
      781 cggcgccggc catcatgttc atgagcttcg tgtcaaagcc cagaaactcg aaggcggttc
      841 catccgcggc ctgcggcttc cccttctcga gatgcaggcg ggtcccgccg ccgccatcgg
      901 gccggtattc ggcgggggcg atatagacgt cgcgcacgag tccgcggatc acgaagggct
      961 cgcgatagag cgcgcccgaa cgctggtcga caaaatacgg cgccctgatt gcgcgctccc
     1021 ggccgccgtc gcgcacggtg aatgcgagcg cggtcgtgtc gcgcccggtg atcccgtcga
     1081 agacgatctc gaggggtccg accgcggtgg tcgcgccctg cgtgagctcc cgctgcgccg
     1141 tcgtcgagtg caggttcgag gtgacaaatc ccacgacgag gacgcccacg ccacagtgcg
     1201 cgatccgcgc agcgatcgcc ctgcgggagg gcgcgcgcac gatcgcatgc agcgcctgca
     1261 gggccgcgag gctcccgaat gcggcgaaga gataggcgaa ggcgttcgtc gtgctcccaa
     1321 cgttaagcgc aatcccgatg ccgatcgcgg cgcacccgag tatggcggcc gcccatcggc
     1381 cgacggcgcg gggccatgcg gcacgggacg cggcgatgat gagcgcgagc aggagcaagc
     1441 cggccgggat ggagaggttg ttgtagaatt cggccttcac ggaggagccc tccgggacaa
     1501 cgatccgcga caggatgggc atggaggtgc cgatcaaaat gaacgcggca tagccgcaca
     1561 ggaccacgac gccacaggcg atgaggttgt caaggcccag cagggattcg cccagcggct
     1621 cggcggtcat cgaggagcga cgccacagga acagggcgat cccgccggcg gccgtcgccg
     1681 cgatgaaaac gagcagatgc gccgagagcc cgaggtcccc aaacgaatgc acggagaact
     1741 tcgacaggac cccgctgcgg gtgaggaagg tgctgtagaa gacgagcacg aaggtcacga
     1801 gcgcgagggc gatgttcgat ttgacgagcg cgcgttttcg ccgatggacg atgagcccgt
     1861 ggacgagcgc cacggtcgaa agccacggga caagcgacga gttctcgacg gggtcccatc
     1921 cccagtaccc gccccagccg agcaccgtat aggcccagta gccgcccagg aagatcccga
     1981 tccccagcga taccatggtg aacagcgccc acggataggc gcgcgccgcc cagtcggtga
     2041 agtcgtttcg gacgagcgcg gcgatggcgt aggcgaaggg cacggtcgcg gccgcgtacc
     2101 cgatgaagag caccggcgga tgggagatca tccagaagtt ttgcaggagc gggttgagcc
     2161 cggccccatc ggccgggacg gcgccggggg cgatccggtc gggcatggcg tcccagacga
     2221 aacgaaaggg cgagtagtcg ggcatcgcga tgataagcaa gatgaagagc atcggcacgg
     2281 tgaggaccgc gacgacgata ttgttttgtc ccgcgcgcgc ccggtccagg accgtcccga
     2341 tcgcgtagag gacgaaggcc cacagcagaa aggtcccctg ttggccggcc cagagccccg
     2401 cgatcttgta ggcgagcggg aggtcgcgcg acgagtagtt gtacacgtag gcgtaggaat
     2461 agttttcggt cacgaaggcg gcggcgagga gtcccatggc cgcggccatg gcgatgccgc
     2521 ttgcggtgaa ggcgatccgg gccgggcggg cagatccggc cgcgcccatg gcgtcgcgca
     2581 gtcggaagac cagcgagatc gcggccgccg ccagcgcggc gatcgtgaga agatggccgg
     2641 cgttcatggg cgctgttccg ccgtgtattt cgaggggcac ttgaccagga gcttttcagc
     2701 cgcgaaccgg cgcgcggccg gatcgtacga tcccatgacg acggcctgct cggcgtgctc
     2761 gaagttgagc ggcttcggtc cgcggtaggc gatgtccatg cgctcgccgc gatcgtccgc
     2821 gatcgtgaag agcatggcgc cgttttcatg gcggaccggg gagcgcgtgt ccagctttcc
     2881 gatgatctgc accgaggcgc ggcccgagcg cgcctccgca aagctcacgt agggcgtgat
     2941 cgcgccgcgc aatgaataaa acgcgatccc cgcgcacagc gccaccccca tcccgatgag
     3001 tattttgtta ttaatcttca tggccctgct caatgcgcga aatcttccgg tcgatacggt
     3061 acaggtacgc tcccagccca atccacacga tgatcgatac gacgacaagc ggaattcgct
     3121 cgcccgtggc cgatgtcccc tcgcgcggcg gttgggtcgg tactgccttc tctgcggcgc
     3181 tgtcgcgcgt gctggcatgc gcggcatcac acggcgcggc tcgatgagta tagccatgcg
     3241 gtgcggccgt atcgaaaaaa agcgtcgctg gaataataag cgccgcaggc gccatcagca
     3301 tgacggtgac gaagatgagt gatttcatga tgatccctcc cggcggtgcg cgatcgatgc
     3361 gatacgggcg cgcaggtgca tgagatacgc gtagagcagc gtgaacgagc agacggcgat
     3421 cgcgagcgtg atctgcatgc ggccgtcgag gtggaccttc cggtccgggt tgatcacggg
     3481 gtccgggtgg agcgacgggt acaggcgcgg catgacgaag atgaaaaacg gcgccgccgc
     3541 catcgcgatg atgagatacg acgcgcagat gcgcccgcgc gcgggactgc ccgccaacga
     3601 cgcgcgcagg gaaaagtagg ccacgtagat gagcagcagg aaggtgatgg acatctcgcg
     3661 cgggtcccag ttccagtacg atccccacgc ggcgcgcgcc cacaccgcgc cggtcgcggt
     3721 cgccaggacg gcgaacagga aaccgagctc ggcggagtgg tgcgccttct cgtcgagcag
     3781 gcggacgcgc ccggtgcggt cggcgagaaa ggcgatcgcg aacgcgcccg cgaccgcgaa
     3841 ggccagcgcc gagacccagg cgatggggac gtggaggtac aggatgcggc tcgactcgcc
     3901 cagcaccgcc gcggggggcg cccacaggag cgacagcgcg atcgagagcg gcatcaataa
     3961 gtagatgctg cggagcgcat aggtgatgag ccgtgcatgt gtcattagtg tttcacctca
     4021 cccgccttcc cctcgactta gctcgggaac cgtcgggccg ccctctccca caggagaagg
     4081 ctccgcgaaa agttactcgc ggcacccccc ttctcctatg ggagaagggg ttgggggatg
     4141 aggtggcgat ctgacgtctc atgtttcgca ttttctgtct atggaagtcc atttcccgcc
     4201 ccgcttttca caggcgtcaa tcttttcggc gtccagtttc gttagaacaa atagcgcaaa
     4261 cagatataga atgaacaatg ttgcgagcga cagggcggca agcagaacgc atttgaaggc
     4321 gattcgcagg atgatccgca tgcgcctgat gggtgtgccg gatgatgttc gcatggcgca
     4381 gcggcgtcgt cgtccgcgaa ggtcgctact cctcccagat gtgctcgaac aggagatacg
     4441 atacaccggc cattgcgccc gaaaaggcaa gcaaaaaaag gacgctgccc gaatcgaagg
     4501 ggccggggcg ctcgagcgag cgagcggtcg cgccgatgag gacccacagc gcggggatga
     4561 cgacgggaaa cgcgatgacc gtaaagagcg cgcccctccc gccggcgcgc gcgaccatgg
     4621 ccccgagcac cgtgacggcg caggcgaggc agaggcaccc cgccgcggcg atcgcgacga
     4681 aggaatgggg cgcgcggacc tcgagttgca gaaacacgac gaacaggggg atggcgaccg
     4741 cctcgatggc cgccaggagc gcgatattga aggcgagttt cgccgtgagc accgtgtggg
     4801 gggtcgcgtg caggcgcaga aagagcgccg tgccctgctc ttcttcccgg acgaaggcgt
     4861 gggcgaggcc gttcatcgcg gcgaaaaaga cgacgatcca caagagcgcg gactggacgc
     4921 gcggcgcggg gacggtcccc gccagggcga tgctcgccgc gatcgtcgcg acggccgcga
     4981 aggcgagcgc gacggagagc gcgtagcggt tgcggaactc caccgtgagg tccttgcgga
     5041 tgtttttcgc gagctcaccc aagcgcggtc ctccgggagc agagcgcggc ctcgtcggcg
     5101 tcattggtgg ccatcacgat gatccgctcc gcgcggcgcg catcgatctc ggcgcgcagg
     5161 agcgcgcgac ccgcctcgtc gagcatggcg cccggctcgt ccaggaagag cgccggcggg
     5221 tcgttcacga gcgcaatggc gagcttgagg cgctgccgca tgccggagga gtagtggcgc
     5281 gtgagcttgt cgcggtgcgg ccggagcgcg aatcgctcca tgagcgccgc agcccgctca
     5341 cgtgacgatc cgccgcgcgc cgcaaacagc agattttcca tgccggtgag atcggggtag
     5401 ggctcgacgc gcgggcccat gaacccgatg cggctgcggt ccgtgcgcgc gtcatcatcc
     5461 ccgcccgccg tgaacaggac ccgtcccgcc gttggccgcc gcagcccggc gaggatctcc
     5521 aggagcgtgg acttgccaga cccgttgggc ccgacaatcg cgagcgactc gcccggggcg
     5581 acggtcagcg tgaggccttc gaagagcgcg acgcgcccga agcgcgccga tacggcgtgg
     5641 gcggcgatcg cggattcggt gtttcgcata acgatgtttc agccggccgg cgtctcgccg
     5701 ccgtcgatct gtccctggat gatgagcagc tcggccttgg cgatgctgtt ggcggggtcg
     5761 atggccagga tctgctccca gaccgccttg gactcctcgg ccttgccgat cttcatgagc
     5821 gcaagggcgc aggtggacag ggcgttcttg aaattcggcc ggagggcgca ggcctggcgc
     5881 gccgcggcga gcgcgtcctc gtagcgcttg ctgttgtaat gcgccgcggc gagcatgttg
     5941 aaggcgtcgg cgtcctcggc gttggtgccg gtgtagtagg agaggtgcgt gatcgcctcg
     6001 tcgtacgcgc gctccttgaa gaagcacttg cccagcaggt ggtggacctt cgcgtcccgg
     6061 ttgtcgatct tgagcgcctg cttgagctcg gcgatcgcgt cgttgacgcg gttgtggtaa
     6121 aggtgctcga gcccgcggtt tttgtactcg aggagctccc ggtaggccgg gtcgatctcg
     6181 acgagcagga aggtgacgtc gtcgcgcacc ggcgtctcgc cgatgaaggt gcgccagtgc
     6241 tcgtggatcg cgtttttcgc cgcgtccagc ggaagcggga aggtctcgat gaacagctcg
     6301 cggaggcgcg cctgcccgta ctcctcgccg tgggaattgc gcgcctcgac caggccgtcg
     6361 gtgtagagga tgaggcggtc gccgaaatcg aggtagtcct gcttctcctc gtacatctgg
     6421 ttggcctcga tcaccgcgcc cacgaacagg ccgttggtgt cccaggtgtc gagttcattc
     6481 gtgcgcgcgc gcgcgacgaa ggccttctgg tgcgaggcgt tggagaagaa gacctcgtag
     6541 gacgggctga tgatcacgag gaaggccgtg aggtactcct gcgtcttgat gatgcggatc
     6601 atctgctcgt tcacctggcg gaggatgtcg cgcgggaact gcagctgcgc ggtggcctcc
     6661 gagaaggtga tcttggccat cgcggtcacg aaggccgcgg ggatgccgtg gccggaggcg
     6721 tcggccatga ggacgcagag gtgccccccc ttcatggggt agatgtcgta gaagtcgccc
     6781 ccgatcttct ccatcgcgcg atagaaggcg accatgcgga tgccgttgta cgacagcggc
     6841 atgggcggca ggatgttctg ctggatgtcc atcgcgatgt ccagctcgcg ctggatgatg
     6901 aggtccttct gcttgagctc gtcgagcgcg ctgttgagct cgttggtgcg ctcctccacg
     6961 agcttctcca ggctctcgtt gaagcgctcc tgcgtctcgc gcgcctcgcg ttccttctgg
     7021 agcaggtcgt tctgcgcgcg catctgctct tggcgcatga ggttgatgcg gtcgcccagc
     7081 gcgagcgaga ggatgatggt ctccaccgca agccccacct gccaggcgta ttcggtcagg
     7141 ggggtgtgcg gcgccagggc gaagaccttg agcccgtaga ggcagccgca caggaccagc
     7201 atggtccagg cgaggagata gaagcgcgcc tcgcggtacc cgcggcggat ctgcacgaac
     7261 cccgccagga gaaactgcgg cgagccgatc atgcacagga tcaccgacag ctggatcgag
     7321 tagtagtagt ccaccacgag cgagagaacg gcgctggtca tgagcaggac cgaacaggcc
     7381 aggatggaat agtagagcgg acgaaacagc tcgcgaaggc gcaggaagga gatgcagaag
     7441 aatcccgcca tgccggcgcc cacgctcacg aaaaaggaga tggaggtgcg gctccaccag
     7501 atcgaatcgc cgaagtagtg cgcggaaaaa ccgttgatgc ccagctgcgt aaggccgtgg
     7561 agcgagacga gggtgccgct atacaggaga tagttccggt cgcgcgtgac gatgaagatg
     7621 atgagattga acagcaccat cacgacgatg atgccgtaat acaggccgaa gacatggtgg
     7681 tcgcgcgtga tgtggtcgcg gtactgtcgc gcctcccgga cctcgagcgg gaccgggagc
     7741 gagtccttgc tcgcgacgcg cacatagcag acggtcggcc gggcaagcgg cggcaggggc
     7801 agggtgaaat ggcggttgaa atttccgctt ttcgatgcgg gattgagccg ccccgatgca
     7861 tgctcggtga agccgcgcgc gccgtccggc gtgtacaggc ggacgtcgtc gatgagggcg
     7921 tagggaagca ccagcagcca ttcccgccgc ggatcggccg tgttgtgcag ggagaagcgg
     7981 aaccagtagg tcgcgcgcgt gtagccgaag ttggggaccg cggcatcgag cggccggaat
     8041 gagctggcaa gcgatggtcc cgcaacgtcg gcgacggtga gcgttcccgc gcggtcctcg
     8101 aggaggtcgc agcgcgttcc gagcgggtag cggctgccgg cgtcggtcag gacgagcgtt
     8161 tcggccgcac gggcgtccgc cggcgagagg agcgcggccg caatcagcgc acaaacaagg
     8221 gtcgcgatgc gcgcggtgtg gtgcaggatc gtggctggca tgagctgtat ttccttcacg
     8281 gtgtttccga tatgaatatc cacccggcga aattttgtcc acaaaataat cgtgccggcg
     8341 gacggtcccg gcggcgcgat gaaggccgcc cgcggggcga agattttttt gatgacgacg
     8401 cggcccccat cgtctgtgat gtcgcccgat gatacgagcg cgaatgagcc accggcgaac
     8461 cccccttctc gagcgcctgc tccccgtctc cggcggtgcg gcggtgatgc tcgtcctgtt
     8521 cggctatggc ctcatcaaca gcttctccat gtcgtacatg gggaacacca gcgagcgggt
     8581 ggcgcagttc attatcagcg gcttcctggg gcatatcgcg ctgttcatgg ggaagatcct
     8641 gctcgcgtac ctggcgatcg gcgcggcgct gggcatcggg gcctgggccg tctatcgcgc
     8701 cgcggacgag ctgttcgatc tggactggtc ggcgttgcag cgcttcgtcg cgaacgcggc
     8761 cgcgggcgtc ctcgtcgtcc acctgtttct cttccgcgac atcatcgcat atccgcaagt
     8821 ctacatcaac aatttccatg cgaaaaacgc gctcaaccgt tttgtcttcg atgccctgac
     8881 ggagcacctg tcgccggcgc tcttttccgt cgcaatctat ttttcgatcg cgctcgccgt
     8941 cgccgcgggg ggcttcctgc tgtaccgctt cgacccccgg atcgcccgat ggggcggcgc
     9001 gctttccggt gcggccgtca tcatcaccct gcttgccgtg aacctcgacc tgtcgcgtcc
     9061 cggcgacgcg cggcgtccga atatgttgat ccttctcacc gacgcgctgc gggccgaccg
     9121 cctgagcgcc ctcggatacg cgcggcccac gacgccgcac ctcgatctcc tcgtaaagga
     9181 cggcgcggcg ttcgagaacg ccttcatcga ggcgccgcgc accttcccgt cgatggtctc
     9241 ctatctcacg gggcagtacg cctccacgca cggcatccgg cacatgtttc ccgccgcgcg
     9301 cgacctgaaa aaagacttcg tcgcgctgcc gcgcattctg cgcgagagcg gctatcgcac
     9361 ggccgtggtg gccgattacg ccggcgacgt cttcagccgg atcgacttgg gcttcgaggc
     9421 ggtggacacg ccgtttttca acttcaacgc cgtcatcgag caggcggtgc tggacaatca
     9481 cgtcttcctg ctgccgtttc tcaccggtcg ggcgggcctg ttcctttttc cggtgctgcg
     9541 cgattcggcg acgttctgcc cgccgcgcct ggtgggcggg cgcgtgatcg agcagatccg
     9601 gaagtcccat ggccggccgt ttttcatcac cgcgtttttc tcggcgacgc actttcccta
     9661 tgcgccgccg cacccgtatt acgcgatgtt tcgcgcgaag ggataccgcg gcccgtaccg
     9721 ctacctgaag cagcgcgagc tctcgctcga cggcgcggcg cgcgcggaga tctcggcggc
     9781 cgacatcgcg caggtgcgcg cgctctacga cgccggggtc cgcgccttcg acgacgccgc
     9841 gggcgacatc ctgcgctatc tctcgaggaa gggcgcgctc gacgacacca tcgtcgtggt
     9901 gctctcggac cacggggaga acctctacga gcacgagttc ggcatggggc atggcgagca
     9961 ctttcgcggg taccacacca tccgggtgcc gctggtgatg cgccatccca ggctcgtgcc
    10021 ccggggcgcg cgggtccgcg atctggcgca ccaggtggac ctcgcgccca cgctgcttgc
    10081 gcttgctgga ctgcccgcgc cggattccat ggagggcgcc tcgctcgtcc cggcgctgcg
    10141 cggtgggcgt ctcgcgcgcc gatacgccta cggcgagacc ggcatctggt tcgacaatga
    10201 ccgcagcggc gttcatttct tccaggagcg gcgcatccag tatcccgaca tcaccgccat
    10261 ctccgaggtg gaccgctcgt tctccaatca catcgtggtg ggcgacgcgt atcgcgacat
    10321 cgtgaacatc gccaagcacc ggtatatttt cgacggccgc tacaagctca tctacatgcc
    10381 gcggcgcgag ggcgtggcct acgagctcta cgacgcgcac cgcgatcccg acgaccgcac
    10441 ggacctcgcg cggcgcgatc cggccacccg cgagcggctc gcgcgcgagc ttttccgctg
    10501 gatgacccga aacggcgacg tggtcgtgaa aaacggcttc gtggtgccga ggtgacgctc
    10561 cccgcttcgc ttcaccgtaa atcggcttga caggatcgtt ttacggaata gaactttctc
    10621 atgaagctcc cgcgttctac catggaggcg cttgcgccgc gcatcatcac gcgctacatc
    10681 atccgcgagc aggtgcccgc gctcgtgatc ggcctcctgt ttttcacctt tatcatgatg
    10741 ctcaaccgca tcttcacgat cgcggacctc atcatcaatc gccgggtgga cggctggctc
    10801 gtctttaagc tcttcctcta tatgctgccc atcaccatct ccttcaccgt gcccatgtcc
    10861 gtgctcatct cggccgtcat ggccctctcc cgcctctcgg cggactcgga gatcaccgcc
    10921 ctgcgggcct gcggcgtgtc ggtgtaccgc atcatcaaac cggtgctggt gtcgggcttc
    10981 gtcgtcttcc tgatggtgct ggccttcaac gagacgctct tcgtgtacag caaccgcacc
    11041 tacaaccagc tgttcgtgca gatcctgcgc tcgagcccca cctcggtcct cgagcccaag
    11101 atgttcacca cggtggggga caagaccatc tgggtgggcc agatcgagcg cgaggtcgag
    11161 cggctggggg acatcatgat ctacaaccgg aaccccgccg gcgggtggga catcatccgc
    11221 gcccaggagg gtctttggaa gcagaacgcg gacggcagca agtcgctcat catgtaccgc
    11281 ggcaagattt tctccagcga tttcgacaag gccacctttt cgtacctgga cttctccaac
    11341 ggcagcgccg aggtgctgat gaccgagagc aagatcgact acaccgagga ccgcgacgtc
    11401 atcaaccccc atgagcacga ctcggcgggg ctgctcgcgc ggctgcgggc gcacccgaag
    11461 tcgtaccgca acgatcggaa cgtcgcgctc aactggatcg agtttttcaa gaagcacgcg
    11521 gtgccgttca gctgtttcgt cttctcggtc atcggcgcgc ccatcgggat cttctccacg
    11581 cgcagcgggc ggggcgtggg gtttggcgta agcatcttca tctttttcgc gtattacgtc
    11641 ttcttcgtcg gcgggcagtc gatggcggtg cggggcgacg tccatcccgc gctcggggtg
    11701 tggttcacca attttctgtt catgctcatc ggcgtgctcc tgatcgtctt caaggagcgt
    11761 ttggacaccg cgaacatcga tatacacgcg ctcgcggggc gctggcaggc gctcgtcggg
    11821 cgattcagga aacggcggga gacgtgagcc ggcacatcga agcaccggga gaaccgttat
    11881 gagagaagtg agcatttggt acctgaccga caacgaaagc ggcgcgtcga tcgcgcgcgg
    11941 catcggcgag ctgggcctgc aggttcggac gatcaccgac ggcgacttcg ccaaggcgaa
    12001 catcatcgcc gacatcatca acctgttcgt gatcgacctc aaggggacgg acctcgaatc
    12061 ggtcatcgac atcatcaagc gcgacgcccg gatcaacggc ttcctcaagt tcgtggtgct
    12121 gggcaagcgg caggcgaaaa aggtccacca ggtggccgtg aacctgctcc acgtggaata
    12181 tctcacgcga ccgctgggcc tgcgcgaatt cacgctgctg ctcgagaaga ccatcgtggt
    12241 ggagaagtac cgcgagatca tgaagctcat ctcgcgcgac gcgcaggagc gcatcgagac
    12301 ctacgagagc ctggtgggga tcgggcggcg cgacgcgttc gacgtgagcg cgcaccgcga
    12361 ggtcttcgag cgcatcatcg agaacgagcg ccagctgatg gccgagcaga agaagctgaa
    12421 cgagaccctg cgcgaggtgt cgctcctgcg ccaggcggag atgttcgacg tgcgcaaccg
    12481 cgtgaaggcc gacgagatgc tctccgagct ccggcgccac gagctcatgg acgcgaagaa
    12541 catcatccgc gcgcaggagg ccgtgatcga ctactcgtcg caggagctga aggacgccga
    12601 gcggttcatc gacgcctcaa cgcgcgtgca ggagctttcg cgcgccgagg cgatccggct
    12661 ccaagaggag ctcgcgtccg cgcggcgcga gaacgagcgg ctctccggcg aggtgaagcg
    12721 actcacgcgc gagctcaagc gcgccgcgcg cgggtagcgt cccgatgcgc cgccccatcg
    12781 cccgcctagg cctcgccctg ctactggtcg cgctgtgcgc cggcgacgcg cgggcctggc
    12841 tcttcgacgc gggcctggag gcgcgcatcg gggagatccg cgcgcgcttc cgtgaacggc
    12901 gcctcatgga gctgtacgtg tgctgcgtcg atccgcgcta caagctgcgg atcgagaagg
    12961 tggtgcagcg catgaaggac gaccggcgga cgggccatgc gatcgcccgc cgcctcgggt
    13021 tcgccaatca cgcagagctt gccgccgcac gcgacgccgc cgtggtggag cggctcttcg
    13081 cgctcatcgt ccatccgcca gccgagcgcg tgcgcatcga caatcccgat taccagcgcc
    13141 tggggcttct gctctccctc ctgttttcgt gtttcgagac cggcatggac atcaccggac
    13201 gcgacgtcta ccgggaccgg gcccgcgtat ttttttccgg cggtcgtctt aaaatggttg
    13261 cgtatttcgt ttttctggac ggtgcttggt atgtcacgga aaaaagcctg ctcggcaatg
    13321 acttctaggg ggcacaggga cgcgtatgga gtgggaaaag gtgctggcga acgccgttca
    13381 ggacgggagg atccgtgaat tgcatcttcg caagatcccc cgcctcaaga cctgcgccaa
    13441 ctggcgcgag gtggagccta tcggctggat cgactacaag atgcgcttcg cccactacaa
    13501 gggcggtatc gtgaagctca atagcaagat ttatttcgtg accgagcaga ccctcaacgc
    13561 gctcagcgag ttcatttcca tcaagttcaa gacgaccctc gccgtcatca aggactagtc
    13621 cccgccgacg gtctcgtcct cctcttcgaa gagcccaatc accgcgtcgc gctcgcccgc
    13681 aaaccgcacc acgtcgtcca ggtgctgcgc gcggatgatg gcgtattccg gcgcgatgcg
    13741 ctccacgatg gcgtccttga gcttgccgta cgagagctcg tcgagcagcg tgggctggtt
    13801 cgagtgcacg agcaccgggc gcgagatccg cacgtggatc gtcttcccca gccattcggt
    13861 gatgagaaac tgcaggtttt gcgggatgcc gttgcgcgcc agcgcgccca gcgcgtcgag
    13921 gaagcggtcg ggcggcatgc cgcgcttgtg cgcccgcacg atggaggtgc ggctgatccg
    13981 cgcgtgcacc atcaggtcct ctttgaggag ctcggtgtgc gtgaggagat ggaagagcgt
    14041 ctcggtcccg atctcacggt gcggaatgat gagcgtgaag tcggggttga tgtaaatcgc
    14101 cgcggacgat ccgcgcgtcg cgtcgcgcgc agcgcgcccg gcgagctcgt ggccgatgtc
    14161 ggtgggcgtc gcccgcccgg cgctcatgga gatcgcgccg cacaggacga ggaagcgaag
    14221 gctctcgtcg aaggagcgca ccgcggcgcg ctgttcgtcg gcgtccgcga gccgcagtgt
    14281 gttttttccg cgggccacca gcgaggccag gtgcagggcg cgcagggact ccggcgtgcg
    14341 cgtcccgtac cgcgtcgcca ggtccagcgc ctcgcgcgca acggcgaatc cgggaatcgg
    14401 caggggcgtg ccgaagagcc cgccgtcgtc ccgcggctcg ttgttccgga gcagcttccc
    14461 gatcagccgg gcgggggagt cgaggtcgcc cgcgatcgcc ttgaggctca cgcgcgcggc
    14521 gtcgcgctcg agggcgatgc agcgcaggcg gtgcaggcag tggagcgcga gctgcgcggc
    14581 ggcgtccgga tcaagcggct cgccgtcgat gtcggtcatg ggcgccatcg cctcggcgat
    14641 gcgcttgcgg tccaccttgc ggaattcccg ctgctgggtc aggaagaggc ctgtcgagag
    14701 gatcacgtcg taggtgtgga gcatgttgag aagaaaccgg caatggttgg acaggagcgg
    14761 ctccccggcg ccgggatcct cgctccggtc cccgagtgtc gccgcgagcg cctcgggcga
    14821 gagcgcgcag tacaggtgaa acggcgacgc caggaccgga aatatctcca tgatgttttt
    14881 ctcggccaga tcgcggatcg tcttctcgcg tgcggcgccg gaaacgacgc tcccgaccgt
    14941 gtcgtagggg atgatcccgc cgcgagcggc ggcggcggca aggagtttct tcgacgcgcg
    15001 gtcgcccgga agcggcagcg ccgggacgga gccggtcgcg agcctcgcga gcatgtcgcc
    15061 atagcccgcg gcgagctcgg cgggatcgcg ggacgcgagg agccggcgca cctcggggaa
    15121 gacgatcagg cggtccatgc ggttgtggat gcgctggcgg ttcttgagga tgtgcgcgag
    15181 gaggcgcttc gcgagcgcgc cggccgcctc ctcgatgagg tcggccggga gccgaagctt
    15241 tttttccatc tcgctgtagg tcgcgccgtt ctcgtcgtcg gccgcggcgg cgagcacctg
    15301 catctcgcgc gggtcgagat cgcccagcac cgcggcaagg ccgtcgtagg tgagcagccg
    15361 cgcggcgagc gcggcggagg cgctctcgga ggaaccggcc gcgcatccga gcaaaccggc
    15421 gatgcgcttg aggtcttccg gagagagcct gccgactgct tcggtgatcc tgctcattga
    15481 tggccgtgcg cgctgtaaaa aattgtggac atggaatagg cgctattcta tatagttgca
    15541 atcatttctg caataacaat tagcctgtag acgccgtttt ttactgccca aggagttaca
    15601 aatgctgcag catctgcggt acggcggaac cgtggccgag cgggcggagt tcccgatcat
    15661 caccggttcc ggcgcgtaca agtttttatt cgtccggttc ggctatctcg tcatcctcat
    15721 ctactcgatt ttcttcctcg cggtcgccat acccttcgag gggacctatt tcatcgggag
    15781 ctttatcgcc acgctctttc tggtgcgctt cgtcaatctc tgcgtgatgt tcgcgcgcta
    15841 ccggaacggg cgcatcgccg tcgacaacgg ggccatcgcc gtcaccgacg cgagcgccac
    15901 cgtcacgctg ccctcgtccg acatcacctt tctcgaggtg aacccgatgg ggaacctcgt
    15961 gattcgccaa aagtacgaca agaccgcctt tccgctggtg ctgctcaccg atgaggacca
    16021 gtcgaagctc ctcgacctct tcgaggacat ggcgcccaag aagaccgcac tcacgaggaa
    16081 ggtgatcgat tttttcgacg cgatcctcgt ggccttcatc ctcgcgatgc atatccgcca
    16141 gtatatcgtc caggcgtatt tcatacccac cggttcgatg gaggacacgc tccagatcgg
    16201 cgaccacctg ctggtcgaga agatcacctt cggtcccatc atcccccgca tggcgggcat
    16261 gaaggacaag gtgcacctgc gcatgctggg cctgcgcgac atcaagcgcg gcgacatcat
    16321 catcttcaag ccgccgcacg aggagaacaa ggacttcatc aagcggtgcg tggcgctgcc
    16381 gggcgacgtc ctgaacatca agaacggcgc cgtgttcgtg aacgggaaaa aggccgtcga
    16441 gccctatgtg cgcggcgcga ccagctatcg cgggtttagc gaccagaaga tcgagggcgt
    16501 ggtcccgccc gggaagctgg tggtcctggg ggacaaccgg gagaactcct acgacggtcg
    16561 ggggttcggc tatctgccga tctccgaggt caagggcaag gccttcatcc tctacttcaa
    16621 ctgggatcag atcaaaaatt tcgacttctc gcggttgggg ttgatacgat agcgcgacgc
    16681 gaccgatgag ccagggcgcg ttcaacaggc gggtctacgt tatcggcatc atcgtcgtcg
    16741 gcatcgcctt cacctacgtg tacaagatgt tcgagctgca cttctcggac cgggtgagcg
    16801 tcggcgcggg ccgacgcgcc aatgtccggc gcggcaccat caccgaccag cacggcgcca
    16861 tcctcgccgt gagcatcgaa aaacactcgc tgttcgccaa tcccgaggag atcgccgatc
    16921 ccgcggaggc cgcggcgttt ctcgcgccgg tcctcgggct cccgcgcgat ttcctgcagg
    16981 cgcgcctcat gcgcaagaag cgcttcgtgt ggctcaagcg catgctcgag gacgatgtcg
    17041 ccgaggcggt ccgccgacgc gccctcaagg gattgcacct gaagcgggag tacgaccggg
    17101 tgtatccgca cgggacgctc gcggccacgg tgatcggctt cgcggaccgc gaggggcgcg
    17161 gtctcgaggg gatcgagtac cgcttcgacc gcgagctcgc gggcgcgcgg agggcgcgcg
    17221 gcatccccgg cgtccgtgct gaggcgccgc cgttgtgcga tatttcactc accctgagcc
    17281 gccccgtgca gcacgccgcc gaggaggccc tggccgacgc ctgccgggcc gcgcgggcga
    17341 agcaggggtc cgccctggtc atcgaggcgc ggaccggccg catcctcgcc atggcgaagt
    17401 accccctctt cgaccccaac gcctatgcgc aggccgacga cgacgagcgg cgcaacttct
    17461 ccatcgtcga ttcgttcgag ccgggctcca ccctcaaggt gttcgccatg gccgcgctcc
    17521 tggagcacca tccggccgtt ctcaaggcgt cctatcggtg cgaggggtac gtcgagatcg
    17581 ccgatgtcct tatcaactgc acgcggattc acgggcggct cgacgccgac ggcgtgatcg
    17641 cgcactcgtg caatgcgggt atgatccagg cgatgaagcc gctcacgcgc gagcacctgc
    17701 acgacctcct cgcgcgcgtc ggattcggca gggccgtggg ggccgacatg ccgggcgaat
    17761 cggaggggct gctgcgcccg gttgcgggat ggtccggcct ctcgaagtat tccatatccc
    17821 tcgggcagga gatctccgtg acctcgctgc agctggcggc cgcgttctgc gcgctggcca
    17881 acgacggcac ctacgtggtc ccctccgtca tcgagtccgt caccgacgcg gacggcgtcc
    17941 cggtccggcg cttctatacc cggactcggg gacaggtgat ccgccccgac atcgcgcggc
    18001 ggctcgtgag catgatgcgc ggcgcggtga gcgaggggac cggcgcgcgc gcgcggctcg
    18061 cgcagtatca ggtcgccggt aaaacgggga ccgcgcaaaa atcctcgaaa cagggcggct
    18121 atgcgcccaa caagtacatc gtctccttcg ccggcatcgc gccggccgag cggccggacc
    18181 tgtgcgtcct ggtcgtcatc gacgagccgg cgggcgagtt ttcgggcggt gacctcgcgg
    18241 cgcccgtgtt cgccggtatc gtccggcgcg cgctgccgct tcgcggcgtg aaggccgcgc
    18301 tcgttccggc cgccgagccg cgcgcgagcg tgttcgcgcc gccgcgcctg ccgatcgccg
    18361 tgatgccgga cctgcggggg atgtcgctct ccgaggcgct gtgggtgctg gtggagcttc
    18421 agaatcggta cggggcgacc ttcgcgatca cgggcggcgg gacggtgcat gcccaggagc
    18481 ccgccgccaa tacggcgctt tcgggaaagg agcggatcac gctgtttctg aaataaagag
    18541 cgcgatggcg tgattgtcac tgcgatgaaa agcgatcaca tcgtgcacgt cgcgcagggc
    18601 ggacgggcaa cgggattatc tctcgatgag cttgaagaag gccaggatga tgccgatgaa
    18661 ggccggcagg aaccagatca ggaaggtcag ccgtttttct attgactcga accgggactc
    18721 gaattttgat tccagcgaat tgatccgagt gtcgattgcg ttgaatcgaa cgtcctgctc
    18781 ctccacgagc gatttttaaa ttctgagagc cgccgagtga ggatgttctc gaagcgctgc
    18841 tcgctgatct cgatgatctc gtccttgatg tgtttgtagt cgttgaagga gatgggagcc
    18901 gccttcttgg tgcgtgtctg catgggcatg ctccgtttca tgagagtatg ctgctcattg
    18961 tacgtctgtt tgtcaaccga aagtaacatc atagatacga acgatggaac cggtatttca
    19021 aaaaaatatg tcgatcctcg aacggagcga tcccgcgctc gcggcgcgca tcgcggcact
    19081 gggcgacgat cccgccatcg tcgtccacga gtcccgtgac ggcaatcgcg tgcccgagct
    19141 gcgcgcggag gggaaaaccg tcctcctgca cagccggttc gatcccgtca aggaggccgg
    19201 gcgcctcctc gccgacttcg atccgcagga atacgacctg gtgatcgtct tcggcgtcgc
    19261 cttcgcctac cacctcgagg cgctcctgcc gcggctgcgg cccgatgcga ccctcctggc
    19321 cctggagcgg cgcgccggga cgctgcgcgc ggcgatggcg agccgggacc tcggcgcgct
    19381 cctggccgac cgccgggtgc ggctggcggt ggacccggac gaggaggcgc tggtgtcgct
    19441 gctctcgtcg gcctcatcgt atcgcataac gttcatcacc catcgcggct cgttccaggc
    19501 cgacccggcc tactacaaca atctcgtgcg gctcgtgaag gcctcgctct cgcgcaagga
    19561 ggtgaacatc gccaccctcg cgcgcttcga gaagtcctgg agcgcgaaca tcgcgcgcaa
    19621 catcaacgcc atcgtcgccg gtcccggcgc gggcgccttc ttcgggcgct tcgcgggcgt
    19681 ccccgcgatc gtcgcggccg cgggcccgtc gctgtcgatg agcatggact tcatccgggc
    19741 caaccgcgac cgcgccgtca tcgtcgcggt ggacacctcc taccgcatcc tgcggaagca
    19801 cggcgtcgag ccgcacttct gcctgtcggt cgacgcgcag ctggtgaacg cgcgctactt
    19861 cgagtgcgat acgcgcaccc gggcggtcct ggtggccgat cccacggtgc acccggcggt
    19921 gctgcgcctg ttccgttcgc gcgcggccgt cgccggcatg gccttcccca tgatgaagtg
    19981 gatcgacgag atcgcggggg ggcgcggcga gctcgcctac ggcggatcgg tgtccaccaa
    20041 cgcctacgat ttcgcgcggc gcctgggggc ctcgccgatt gtcctggtgg ggcaggacct
    20101 cgccttcacg ggcgggcgcg cgcacgcgcg cggatcatac cttgaagagg aggtgtacct
    20161 gcgggtgggg cgtttgtgga cgatggagtc gttcaaccgg cgccagctca cggcgctgcc
    20221 gaagatcctc gtgaagggca tccgcacccc gtcggtgcac accaaccaga agatgatgat
    20281 tttccacggc tggttctccc ggcagaacga cgcgaacctg gtgaacgcga cctacgacgg
    20341 cgcgcagatc gccggcgtcc gctcggtcgc gcccgaggag atcgcgatcg gcgagccgcc
    20401 cttcgacatc gcggcgcggg tggacgaatg ctacggcgcg tggagatccg gcgacggcga
    20461 cgccgcatcg gccaaaaaac acttgctcaa aaaggtttcc gcgatgcatg atgaactcca
    20521 ggcgctgctt ccggtgcttg cgcgcgccga gaccctttcc ggggagctcg tcgcgctcgt
    20581 ccgtcgcggt tcgcgggacc gctcgcggtt cgagtccatc atgggccggc tggccgaggc
    20641 cgaccgggcg gtggagtcca cgcgcgacct gaaggacatg atcgggttca ccattcagcg
    20701 ggtgatccac accattaccg aggggtacga gatcgacgag gcggagggtg tggccgggga
    20761 cgacgagcgc gcggcgctcc ggtcgcagtt catgtaccgg ggactgcacg agggcgcgcg
    20821 ctacaatcgg aagctgttgc gaaccatgat gacgctgctc gcgaagggag ggagccaatg
    20881 aatacgaacg ttatacacgc gatcgtccat atccagaact ggggggagag ctccggggtc
    20941 atggaggcgc tcgtgcgcag caagaacgta atctcggtgt tccacgtgat ggggcgcgcg
    21001 agctacctgg tggacgtgaa cttcgacagc aaggccgagc tcgaggtctg gatcaaccag
    21061 gtgaaggcca tacggctcgc ctcgggcgtg cccgcgatca tcgccattcg gacgcagaag
    21121 gtgatcgagg cgcacaagca gaagcgcggg ttcgacctgg ccagctaccg caatctgggg
    21181 cggatgttcc attttttcct gaacatcgac gtgccgcacc acgacgagga gaccgtggcc
    21241 ctgctgcagg ggtccgacat cgtgcacacc gtcctgcatg tccagggcga gcattccttc
    21301 gtcgcggaga tcatcaccga gcgctacgac gcctacaagt cgctgctcaa gtcgctcaag
    21361 ggcgcgggcg gcgtcaacca cctcgagacg caggaggtga ttgcggtgca caagtaccgc
    21421 aaccaggtct tcgacgagac cgggaatctc acctacccgc gcgaggatat ccgggaaatg
    21481 ttttcgttgt aaccccctcc gtgttgatac ggtaaaagga tgccaatgaa caagaagccg
    21541 gcggagccgc atatcgatga tcccgtgaat tacgcgcgcg aggtggtggg gcgcgatccc
    21601 atggccacgc acctgggcat cgcggtcgag gaggcgcgcg aggggtacgc ccgcacggcg
    21661 ctcaccgtgc gccccgagca cctgaacgca gtggagcgcg cgcacggggc caccgtgcac
    21721 gccgtggccg accaggcctt cgccgttgcg tgcaattcgc tggggacgct cgccatcgcc
    21781 gtgagcatga agatcaccta cgtgtccgcc gcgcgcgacg gcgagcgcat cgtggccgag
    21841 gcgacgcccg tcaatatcgg ccgcaaggtg agcgtgtggc ggatcgacgt gcgggtcgcc
    21901 ggcggcgcgc tcgtcgcctc ctgcgagggg atcgcgtatc ataaatgacc gagcggccgt
    21961 ccgcgccgac gcgatttcgc gcgcgggaca ggggcgcttc gttggcccca cagtgcgcga
    22021 cgctgggctg gcgatcaccc tgtccgggtt ggttccccgt tagccatgcg atgatagcgc
    22081 accgtcaata acccttcgtt cattctcctc catcccgtcg gccggaagat agatcgcaaa
    22141 aacgctaccc gctcccggtt tgctcgacac atcgacgaaa ccgccgtgct gcgcgacgat
    22201 gccgtagacg atggaaagcc cgagcccggt gcccgctccc ggcgtcttcg tggtgaaaaa
    22261 cggatcgaag atcttctcct tgatctccgg ggcgatcccc ggcccggtgt cggacacctg
    22321 cagcacgtga tatcgcccgg gggcgcatcc cgtgcgacgc tgggccatgc gcgcatcgac
    22381 gagcgccgtg cgggcctcga tgatgatgtc gcccgcgtcg ggcatggcgt cctgcgcgtt
    22441 gaggaccagg tttagcagca cctggacgag ctgattctcg tcggcgcgca ccatcaaggc
    22501 ccgatcgccg atgcggacat cgaccgtgac gttcgttcgc acgaggcgcg cgatcatgct
    22561 gccgaaatcg gcgatcaagg aattcaggtc gatcggtttc gtttccaggg gctgccggcg
    22621 ggcgaaggcc agcaaccgct gggtcatctc ccgggatcgc atggccgcct cgctgattcg
    22681 ctcgagttgc tcgcgcgcct gcggctgtgc ggcgagatcg attttcagca tgtcggcgta
    22741 gccgatgatc accgagagca tgttgttgaa gtcgtgggcg atccctccgg ccagcattcc
    22801 gagcgattcc agcttttggg agcgaaacag ctgctgctgc agcgccgagc gctcctcctc
    22861 ggcgcggcgc cgttcggcga tctcattctc ggcccgttcg atggctcgct ggttgatcgc
    22921 gtacacattg tacagtgtga tggaggcgat gaccagcgcg agcgtgttgt cggccaggaa
    22981 atcccagaag gaattaaccg acaggtggag ctcgctccgg acatggaaca tgaacgcgta
    23041 gagaatgatg atattggcgg cgacatagat gatgattacg cgctttttcc gcagcacaac
    23101 gatcgggagg atcgtgagcg ccgcgaagac cagcgagatc gtatcgaggc gcgatacgga
    23161 ttcggccgcg tcgaccagca tgacggccca gatgcacgtc agggcgctga ttaggaacag
    23221 gtgcgcggcg acacggaacc acccgcgcac cagaaaaaca atgaccgcac cggtaattgc
    23281 catgatggcg aatgacggag cgagcaccgg caggatgatg gcgttgcggg agaggggatt
    23341 gatgagctgg agaaaaccgg tgtaccagat cccaatgggc atgatgatca tgacggaaaa
    23401 acagacatac aggagaaagc gcgccttgct gcggagcagg tagggggcgt tatcgtagcg
    23461 acgaagcagc cgatcgatta cggtgttcat gagtgttttt cctccctccg ttcggatcga
    23521 cgcaagtcct gttctcggcg tctgctgtta agtcttcgag cgccggcccc ctagagccgg
    23581 atgattccgg ccagggcaag cgagaatgcg atggcggata tgacgaacgt gatcagcccc
    23641 gcaaggatgg gaagcaggcc cgttcgctcc agatatgatt cttccggccg ggccggattg
    23701 taataggcgt gaacggtcgt tccctccggg tacctgttga cctgtttgtg ggcgattgac
    23761 ggaaaagagg agctccagtc tccggatggt tgtatgcgat tggaggcgta ctccgtcgcg
    23821 tccaccgtat acgtgtagga aatgtgcggc tgatattgaa cgccgtcgga atcgtcgatt
    23881 ttgtgtatgt gtgatgcggt gatcgtcgcc cgcgtggcgc tccatcgata cgatcgcagg
    23941 tggcgtatca gatttcgcag gaaatacatc gtacccaata cggcggcgat gatccctccg
    24001 gcgtgaaaat agcgggcggg cagctcggga acggcgacgc cgtatttcgt gatgccgacg
    24061 gcgatgccga gaaggacgat gacgaccagg atgttccatg gtcggaaggc gatacctccg
    24121 ggtttgtttt gttccatatg ttcctcaaca caccggtcgt agtgccgaac ctgcctcatg
    24181 cgtgtcggtg cgaatacggc tccgcgcgca atgcgctcaa cgcgtgtagc gatgcggcgg
    24241 ttacggctcc ggcgaatggg cgccgcagca ctggtcctcg tcggtgcggt aaaattcgaa
    24301 ctcctcgagc atcgccaact ttggcaacac attgttctcc acgtccacct gaaaaaaacc
    24361 ggggaagatg tctttgttgg cgacgatgaa gtcgaacagg ccgccgtacc ggccgtcgat
    24421 atcgacgcgc acggacacgc ggcgctccat gccgcagatc atgaactcgg ggagaaggaa
    24481 ggggcagaac tcgagcagcg agcggtcggc cagatcccgg tacaagcgcg tctgcggcag
    24541 gtaggtcaag agcgacggca ggatgtgcac gcccatgccc cgcatcgcga tcatttgaaa
    24601 gacgctctcg ctgaagtcct tcatcgtctc gaagggaaag ccccagacga aaaaggcgtc
    24661 cacggaacgc atgatccgtt tcgccagggc caccgtctcc acggccgtct gcgacgtgat
    24721 gtctttgcgg atcatgtcga gaatcctgtc cgatccggac tcgatgccga agcgtatctc
    24781 cacgcagccc gtgtccgcca tcgcctgcag tgagtccctg tccaccaggt cgacccgcgc
    24841 gaaggccttg taggtgatgt cgagcttctc cttcgcgagg agccgggcga agcgcatcgt
    24901 ccgctcgggg ctcgagatga agaactcgtc ctggaagaga aactgccgca cgccgtactc
    24961 gcggtgcatg tggaccatct cgcccacgat gttctcgacg gaccgcagat ggggcctcca
    25021 gccccatatg ggcgcgatgg agcagaaggt gcattgatag gggcaccccc tgctgccgag
    25081 catgttatga ccggcgtagc gtttgaagtc gagcccgtcg tatgacggca gcggcaggga
    25141 atcgagctcg gcgatccgct cggccggcgg attgccgcgg atcgcgccgc tttccctgta
    25201 gaagatgttt ggcacatcgg ccagggggcc gccgccgcgg agggccgcca tgagcagggg
    25261 aacgctccgc tcgccctcgc cgcgatggat cacgtcgatc gaggggacgc gctccatgag
    25321 ctcgcgctcg atgtcgatgg cgcccacgcc gccgatgacg aaaactctgt ccggatagcg
    25381 ctccctgagg aagggcatgg cgtaggccac gaagggcatg aggttcgcca tgcagctgat
    25441 gcccacgacg ggcgccgggt cggccaggaa gtcgcaaaac gcctcaggcg tgaagatggc
    25501 gtcgccctcg aggagttgat aatcgcggaa atcgacggcg aacccctttt ccgtgaggac
    25561 cgagacgagg tacagcggtc ccaggggcag gtggttctgg cgccgcaggc tgccgtcaag
    25621 gtacttgacg tagagcatgt tgaggttgac gagggtcagg tccatgcgtc gctcatcgaa
    25681 tcatcgttct gcgcgataca cgcgatccta ttccagaatc atcttcttga ggcccggaac
    25741 gttcagctcg aagcgtattt cgcagttgtt gtctttgtca taggtttcgc cgacgaccgt
    25801 ccccttttcc ttgacgagcg cgatcacctt tttccagtgt ttcgcatcaa cggaatttcc
    25861 gtcatcggcg agcgcgcccc ccgcggcgcc cgagagcatc gcacccttga acttctcgag
    25921 catgcgatgc tgggcggaca gtattcccgc agcccgggcc gaatccttct gggccttcgt
    25981 tcgggctttt tcatcgttca ttttctcgag ctttttttgg ggcgccccca gggagatcac
    26041 cctgaaggtg tcgttgttga tccatccctc ccggtcgtgt tcggactggt cgctgatgtc
    26101 gataaacgtc ctcccacacc cgacattcgc gaacatcgcc gaggtgacca gcaggacgac
    26161 cgatgccgcg atccgccagc tggcgaggct tgtgcgggtg acgcccggga tggaaaactc
    26221 gcctatgatc gtatcgagct gggtgtcgct gatgctggtg atgatcgccc gctccgagtc
    26281 gctgaacgcg attcccgaat catcgagggc ccgctcccgg tcttcccgca acaatcgccg
    26341 gaacccggga tcgtatttcg ccatgaggag cagcgcctcg atgtttacct tgttgaaccg
    26401 cgttaccacg ccccgctcgg agggttccgg tcccacgatg gtgagcttgt cgtccatatg
    26461 cgatccctcc ttcgatcgag ctccggcggg cgatgatcgg cgcggtcgtc cgcacccgac
    26521 gcgtctcgcc cgctatcgac gttgtgcgcc atgcctcggc gcccgccgtt atgtcccgat
    26581 ggcggtgctg aaaatctcgt attcgccctc gaaccatcgg cacgtgaggc gcaccctcca
    26641 ccagaccatc acgccgccgc ttacataagg ctttctgttc aggaacctga cgcgcagcct
    26701 gtcccacgtt tttgcatcgg cgctgacgta cagcgccgcg tcgcggatcg tataataata
    26761 ccgcttgctc ccttcggaaa tttcggaccc tttcgttttg tcgtggtcat gggtgatcgc
    26821 atagaccgaa ccgtcgtgtt ccctgagcca ggtgatcacc gcggccggta cgatgttttt
    26881 cgggtggttc cggatatatg cccgggtcat ctgcatctgg gggatgtcga atttcgatgg
    26941 atcgaaatat ttcggctcgc catggacgat ggcaacgccc ttgttccaag tcgtcgagtg
    27001 gccattggcc ggtgccgaaa ggcagatcga catggttgcc acaagtatgg ttgcagttgt
    27061 tctttttttc actgcactat gccccggtaa tgggttcgtt gcgttttgtc ggcacgagtt
    27121 cctgtcggat caagagacta ggatatggga cgaggagcgt caagggtttt tcggggccgg
    27181 atggcgtctg cgatacgcgc ctgtaggcgg gtgcggaccg ttacagtcgt gctttttggc
    27241 cgatagcagc ggcaccgccg cgtaatcaaa caccctcgaa aagaattctg cttgtcagat
    27301 ggcgcgcggg cggataccgg ttgcccatgt cacggatagc gatatgccgg cgcgcgctgt
    27361 gtgcgctatg cgccatcttg gcgacgatca cgctgtcggg cgcgcaggag aaatccgcgg
    27421 atcgcccctc gacggacgcc ccgaagcgcg aacgccccgc gagcacctcc gcgcgaagta
    27481 aaaaagcgcc cgagtcctgc gacgccgcct gtcggccgct ctcgggatcg gcgtacaatc
    27541 gctgtgtggc gcgctgcgaa aagtcgggcg cacggcgcga tgcggcggcg ccggagggaa
    27601 cgcgttcggc gatggtcgcc tgcgtgaagg gctgcggggg attgtcgggc ggcgccaaga
    27661 ccgaatgcct gcggcgctgc cgcgaaaccg acgcctcgcg gcgtccatca tcatcaaacg
    27721 aggccgtctt caaggagtgc gccgagcgct gcggcaccct ctccggtcag gcgcgatacg
    27781 gctgcatgcg gacctgcgcg agccatcgcc gggagcaggg cggcgatcgg gacgatgcga
    27841 agtcggtccg ctaccgcgag tgcgaggggc gctgcggcgt catcgagggc cccatgcgct
    27901 acaagtgcgt tcaaaactgc gtgcggacat cgcgatagca tcggaccacg gagcgtgtcg
    27961 aatgatagac ggtaaaccac gccatctccc ggagcgcgac gagttcgacg agcggatggt
    28021 gtcgatcgag agcgccatct tcgacctgca gatctcgctt ctgcaattcg tggccaatct
    28081 cgtggcctcc ttcggcgacg acgcgggcgt cgacattccg gtgcgggtgt tctccggcga
    28141 cgactacgcg atcgtgcccg cgaagcggat ctacttcgac cgcgcggacg agctgctgaa
    28201 gatcgagctg gcggacggcg acgagatcgt cgcgagttcg ctcgatattg gcatgctgga
    28261 gcatgtcgcg cggcagctcc tgtttcggcg cacgtcggac cgcgcgtacc gggacctcgc
    28321 cggtgacgac gaggtccatt gacggcatgc gccgtcgcgg gggaattaat ccttgacatt
    28381 tctccatcgc ccgaacccaa ttcccgcaat atgaaagcga tcaggggcct tatcgccgga
    28441 tgcgcggcgc ttgctctgtc ctgcggcggg tttatccgcg cggaggacgg caagcggatc
    28501 gcacggctcg agcagtacgt ttttgtcgcc cggaaggacg tgtccaacga ccagcggcgg
    28561 ctgcgcgcgg gacagcccat ccggctcttc atccgcgccg aggacaagtg gatcaaggtg
    28621 tacggctacc cgcacgatca gaacatcctc gaggccgacc gggtgctgct cttgtatctc
    28681 tttcccgacg atttcaaggg atcgaaatac gatcacgatt ttttcatttg gcgcctggcc
    28741 gacgaggtcg tccttctggc cgatcaaccg gttgacaaga agaaggacgc gaagcaggac
    28801 gcgaagaagg gcgcaccgca ggccgcgaaa aaggacgtgc gatcggacgc catcgagcgg
    28861 gagcccctgg atccgcggaa gaagaaccaa taacgaaagg gttgaccatg tttgaaggac
    28921 tgcacaccgc gctcgtgacc ccgtttaagg acgggggcat cgactacgac gcgctcgaac
    28981 ggatcgtcga gctccagatc gccggcgggg tcgacgggat cctccccatg gggacgaccg
    29041 gcgagtcccc gacggtatcg tacgaggagc acaaggaatt catcaggcgc gtcgtgaaac
    29101 tcgcgcgggg gcgggtcaag gtgatcgccg gcacgggctc caacgcgacc gacgaggcca
    29161 tctgcctctc gaaggccgcg gccgacgacg gcgtcgacgg gatcatgctc gtcaatccct
    29221 actacaataa gccgccccag cgcgggatgg tcgcgcactt cgccgccatc gcgcaggcgg
    29281 tgccgctccc ggtcatcatc tacaatattc cgggccgcac cgggatcaac tttttgcccg
    29341 agagcctcac ggagctcgcg gaccggtgcg cgaacgtggt cgccgtgaag gaggccaccg
    29401 gggacatcgc gcagatgatg cgcaccatcg agcttctcgg agatcgcata acgctgctct
    29461 ccggcgacga caacctcctc ctgccggtcc tctcgatcgg ggggcggggg gtgatctcgg
    29521 tgctttccaa cgcgtttccg gccgaggtga agcgggtgat cgtcgactac gaggcgggcc
    29581 gcatcgacga ggcgcgcgcg aacttctacc gcctgctgcc gctgtgccgc gccatgttcc
    29641 tcgagaccaa tcccatcccg gtcaaggcgg ccatggagct tttgggccat tgctcgtccg
    29701 atgtccggtt gccgctcatg cccctctccg aggagaaccg ccgcgcgctc aagcgcgcga
    29761 tcgacgattt cggggcgtcg cgatgagccg gatcgggata aacggcgcgg cggggagaat
    29821 gggccgggcg gtcatgggca tgctgctcgc gcgcggccat tcgatcgcct tcgccttcga
    29881 cgccccatcg acgcccgtca tcgggaccga cgcgggggcg ctggtcaacc gcgggccgat
    29941 gggagtgacc gtgtcggcca tcgacgggag cgcgcttgcg ggggccgacg ggatcatcga
    30001 tttttcgtcg cccgcggcct cggagctcgc gctcgcggcg gcccgtgcgc actcggtgcc
    30061 catcgtggtc gggaccaccg ggctctcgga cgcgggtatg cgcctgatcg acgatgcggc
    30121 gcgcgacatc cccgtccttt tctcgccgaa catggccatg ggcgtgaacc tcctgttcaa
    30181 gctcgccgag accgctgcgc gcgtcctcgg tccgggcttc gacgtcgaga tcgtcgaggc
    30241 gcatcaccgc atgaagaagg acgcgccctc cggcacggcg aagcggctcg tcgaggtgat
    30301 caagaacgca tcgcccgcgc tcgcgaacgc cccggagatc tccgggcgcg agggcatcac
    30361 gggcgagcgc tccgatgccg agatcggcgt gctggcgatg cgcggcggga gcatcgtggg
    30421 cgaccacacg gtgcatttca tcggcatgga ggagcgcatc gagctcaccc atcgcgcggt
    30481 gagccgcgac gtcctggccc gcggggccgt ggtcgccatg gagtttctca tcggcaaaaa
    30541 acccggccgg tacggcatgt acgacgtcct ggggctctga tggaccggga caaagcggaa
    30601 cagcggccgg acggcgacgc gcccgccgag gacctgaaga agttcgacga gaacccctat
    30661 cagccggacc gggtgaagcc cgaggacgtg gtccgcatct tctacgagcg ggacacgccg
    30721 ctggtgccgg tcgtatccaa ccgcaacatg ctcctgggcg tcctcctcaa ggaggacgtg
    30781 gtcgccgagc tgtccgacat cgagcgcgtg aagcaccaga agatcgacga gttcgtctcg
    30841 aagctttcgc gcaaaatgac cttcgacgag ctcctgcccg cggtcgcgaa caaccggagc
    30901 ttcgcgacca tcaacctctt cggggaggtg acgggcgaat ggacgcggct ggatctgctg
    30961 gccgccagcg aaaagggcgg gggaagcgcg acggcgatcc ggcgcgaggc ggagaaacag
    31021 cgcgaggagc aggcgctcga atggatgatc tatctcatcc tggagcacct tccccgcgcc
    31081 ctgtacgccg tgaacgaccg cgggaagacc attttcttca acagccactt tgaggacctg
    31141 gttgtggcga acaccggagc ggagatcgat gtcgatgcgg ttgaaaaggc cttcgccgat
    31201 accgacgcca acgagttctt ttatcggccg gagggcgacg agatattctt ctacaacaag
    31261 gagctcgaca tccactacga aaaggtgccg atgacaagcg gcgagcggac cgtcgggttt
    31321 ttaatctact gcagccgctc gaagaccgat cacgcgcgac ccgcgacgcc gcacgtggag
    31381 accgcgggga tgtcgctcaa tcagatgctc gacgccttcg tgcgcaaggt gctggtggac
    31441 gccgtgaaga cgcacggcta caatctcgag acgatcgcca aggagctggg gatatcgcgc
    31501 ccggcgctgt tgaagaagat acagaaatac cgtatcagca tgcagggcgg ccattcgtaa
    31561 aatgtaatga aaaaatccca ttttttcttg actgaaacgg gccgcggaat ataatagcgg
    31621 aaaatcgcag ttgatgtatg tcctccacac ctctttgaat acatagcgca catctactct
    31681 atctgtttcc gtggttaagg gtactggatt tcaatacggg taattcctga gtgaagcgcc
    31741 atacaaacaa tgcctccggc cccgctgggc atcgggggaa acgctaaatc atacgccgct
    31801 catgccgctg tccgcagcct cccgccggac gccgcgcgtt tttccgccgc atgcatggcg
    31861 cagagcgcgt ctcatatagg aacgacaccg gtgggatata cgggacgaat gggcggtaac
    31921 cgcaattcgc ggatcgcccg cagtcggtgc gccggcggcc caatgcaata ctacgaacgt
    31981 gaagggatgg tatgacacga cagacttcaa acgcagcagc caacgtgaag aacgggaacg
    32041 gcgatgagac ccggaggcgg ggccccgccg aaaacaagtc gggcgacgcg aacggcgcga
    32101 acggcacgag cggcaatggg aaaaaacccc aggcctctgc cgagcgcctt gaccttgccg
    32161 agctgaagtc gaaaaccatc aacgacctgg tga
//
LOCUS       DAORHF010000010        31878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_805_length_31878_cov_5.940860, whole genome shotgun sequence.
ACCESSION   DAORHF010000010 DAORHF010000000
VERSION     DAORHF010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 31878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31878
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_805_length_31878_cov_5.940860"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKJ16_01825"
     CDS             <1..418
                     /locus_tag="PKJ16_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV45749.1"
                     /translation="RFREAVAQAHDHHERFVLMAEAALLVVVCLCYGFAKTLLPETPA
                     LIVAGTCFVLDQLLMWAGMARATWLKKIARAPEDVSPTLAMGVSIDHIFSIAIALASG
                     VLWLTLGYQYVFLLGAAIALVNLVSASYVRVPSRSA"
     gene            462..1112
                     /locus_tag="PKJ16_01830"
     CDS             462..1112
                     /locus_tag="PKJ16_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV45750.1"
                     /translation="MKIIALSDLHGNLDRLDAIADELRAADLLLVSGDITKSGSADEA
                     AGIIRTLQEYNAALLAVHGNRDFPEVATALDEAGVGLHARGTIVDGIGFFGAGGGRTT
                     RFHTACEYSEGEILEFLQRGFAAVAGARRVVLLSHMPPKGVRDRMFLGFRAGSAVVRD
                     FISGNRVDLCVCGHIHEARGTERLIDTMVANAGSFRNGWYARIELDRTVTVTIERA"
     gene            complement(1123..2304)
                     /locus_tag="PKJ16_01835"
     CDS             complement(1123..2304)
                     /locus_tag="PKJ16_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV45751.1"
                     /translation="MNRPRKLYLDANLQIVFWVTLMVVMGVSNITPAIPAMAKALSIA
                     DREIGLIISVFTLPGVFLTPVFGFLADRYGRKRVLVPSLVLFGIAGTMCFWCESFASL
                     LALRFVQGIGAASLGAVNVTIISDLFSGRERTAALGYNASVLSIGTASYPAIGGALAM
                     LGWNYPFLMTAAAIPVALAVLFLLNNPEPHARPEIRAYFAAVFRVVRARQVVGLFIAS
                     LAAFIFIYGAILTYFPILMAQSFGATALAIGLFLTAMSVANAITSSQLGRLARRWSER
                     AFVIAGFSLYAVVCALIPFAASYPLMLVPVVIGGMANGIVMPSLQALLAGAAPPAQRA
                     AFMSFNGMLLRAGQTGGPVLLALFYHFGGMRFTFLATAACMLVMLGVIAWLVRDERAA
                     E"
     gene            2516..3154
                     /gene="thyX"
                     /locus_tag="PKJ16_01840"
     CDS             2516..3154
                     /gene="thyX"
                     /locus_tag="PKJ16_01840"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HNV45752.1"
                     /translation="MELSRPVVKLLAVTPNAEALIEEAGRTCYLSFDKMTDESGAGFI
                     RGAIRRGHLSIIEHAAATFRILGASRAFTHQLVRHRLASFSQQSQRYVSEDEFNYIVP
                     PEIAANDGAARVYRGFIEQAQAAYRELRALGVKKEDARFVLPNALESQIVFTANFREL
                     RFIFSLRLQKSAQWEFRRVCFDMLAIMQREAPAVFGDFVMDEADLTAASNLT"
     assembly_gap    3210..3309
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3310..3937)
                     /locus_tag="PKJ16_01845"
     CDS             complement(<3310..3937)
                     /locus_tag="PKJ16_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45753.1"
                     /translation="MQLTDKQRLAARTFPLQFILRTEGISYFFIVPMIVFYVWSNVNL
                     THDQLVIFLVSVAVAFPISFITTQINNFIVIAPVLRYFRKLLSGAEVPEDEYNRAHRR
                     FIMLPFLHSFGAFFRWLFGLGMGVLPTVYFGNTNSEQTFNLWMTIVINAPLGTVLYFI
                     LTELYTQKLMRLGLFPAWPRERLNLHMNIFPRLTSSIIVITLLPFLMLL"
     gene            complement(4243..6072)
                     /locus_tag="PKJ16_01850"
     CDS             complement(4243..6072)
                     /locus_tag="PKJ16_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV45754.1"
                     /translation="MQLTDKQRLAARTFPLQFILRTEAISYFFIVPMLTFYVWSNLNF
                     TRDQMILFLMFAAGTFIVSFTTTQINNLIVIAPVVRYFRNLVKGADVSEHEYNRAHRR
                     FLLLPFVHSFGAFFRWVGGLSFVIVPTMIFADINSVQSFNLWMCVLINAPLGTVLYFI
                     LTELYVQKLMRLGLFPAWPRERLNLHMNIFPRLTSSIIVITLLPFLMLLTFFIIFIAD
                     LQVDKTLAYVKIGIIGGIGVIGAVLVSYVLTKSIVFKVRIIQGFLKQVGEGDLFAPAQ
                     KIAVLDELTQINKSVYGMKENLRAMVHAVAETARELSASSEKLIQSSNALSDMARDEA
                     AIIEEASSSFEEMSASFEMNLSNVKSQLDSAGAVKDDISRISEKGEALAKQTWTLSER
                     AGKSVKIAEDGEGLMNRSVKTIGNMVGYMQNIDEMAGQINDIADQINLLALNAAIEAA
                     RAGEAGRGFSVVADEVNTLADQATKLAGNIKKSVSENSAKINTELASLNNTVDAFNSM
                     KASILDIDAVIGQVFDFTQELIRMNDEIKGKVDRMNSLANDIYLASAEQQTTTAELTR
                     AVNSINELSQHTAESAEFVSHSAKSLNDSAQGLQAQIAKFRVS"
     gene            complement(6292..7137)
                     /locus_tag="PKJ16_01855"
     CDS             complement(6292..7137)
                     /locus_tag="PKJ16_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNV45755.1"
                     /translation="MPLNKDIVGKSYTAEPTEVTAHGSIFYALAYNEDNDAYFDGRRE
                     GGIIAPPMYAVYYAGRPIVNIMNDPETGMNMQKVVHWSQEYEWLRPVRPGDVIRTEAV
                     ITHVTVRRFGGLLGWETVSRNQRDEEVVKTKWEFFDLSAGDRSVSDPRERPQRPAEFL
                     WERTMTVRNGQTFIYAEASGDNNPIHLDDAAAKGVGLDGVILQGLCTMAFAHKAVVDT
                     CAGPERDPLRVRKLKVQFARAVLPGQTLTFKGFRMEDAEGKIGLLARNDAGKDVLMNA
                     WCEVV"
     gene            complement(7446..8402)
                     /locus_tag="PKJ16_01860"
     CDS             complement(7446..8402)
                     /locus_tag="PKJ16_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HNV45756.1"
                     /translation="MIPPAKYYRRKVRNALQTALLFALMAALLGAIGYIMSGPFGLVV
                     ALVFALAIMAMSPAVSPRMVLKMYRARALSEYEAPYLYRVIEELARRADLPAAPVLYY
                     VPSSIMNAFSVGDRTNAGIALTDGLIRALNPRELAGVLAHELSHVRHGDLWIMNLADS
                     LSRFTNVFSTVGIFVLVVSLPLYLAAGVMVPLPALAVLMGAPTLAVLLQLALSRTREF
                     EADLGAVALTGDPRGLASALGKIERYPMRLWDMILMPGRKVPGPSALRTHPETEKRIA
                     RLLELAEETQRHIEHVRDATVLPERYPPVTRAPRWRSGGIWH"
     gene            complement(8510..9796)
                     /locus_tag="PKJ16_01865"
     CDS             complement(8510..9796)
                     /locus_tag="PKJ16_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HNV45757.1"
                     /translation="MEDLKKAYKTVMDDHFPETMTIDFGGQKLVYKKRVWRIEDGGAL
                     IEKGLRYGENPGQESAMYELVNGNLVLGGCEFIGAGNALVSGIDEAMMIQSGKHPGKI
                     NLTDIDNSLNVLKYLMAKPACVIVKHNNPCGVAYGSTAAEAYDRAFRADRIAAFGGCV
                     ALNSAVDRETAELVAKQYIEVIAAPDYTGEAVDILKKRKNLRIVKIARIAELERYRDR
                     RFVDFKSMIDGGIIVQQSPVNRVKTKDDFAPAAAEYKGQKYAIDRLPTEKEYEDLLFG
                     WFVEQGVTSNSVLYVKDGCTVGIGTGEQDRVGVADIAVYKAYAKFADLICFDRHGISY
                     NELALAVEKGERDESELDDIDDETEDAKGGLVGSVMVSDAFFPFRDGVDVGLRQGITA
                     IVQPGGSERDFESIVACNEADPKVAMVYTGQRAFKH"
     gene            complement(10003..10749)
                     /locus_tag="PKJ16_01870"
     CDS             complement(10003..10749)
                     /locus_tag="PKJ16_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV45758.1"
                     /translation="MNLLFEMINSIACIIFIVINTILWGSVSVTSVVYSPSGRVAHFC
                     MRWWSKTTLWFCRVKVDVDGLEHVSRDRVQIFASNHASHFDIFVLSAVLPVKFGWVSK
                     KILFFVPFLGWHMKLQGYVSIDRGNRASAIKSLDEAAEKIKGGNRIAIFPEGTRTRSG
                     ELQPFKKGLFHLCVKTGVPIVPIFINGTYGILRPESLAVHPATVYVKIGSELPTAAYS
                     PERIEDLMNDLMARFIELRKEAEEKRVRWS"
     gene            10929..12140
                     /locus_tag="PKJ16_01875"
     CDS             10929..12140
                     /locus_tag="PKJ16_01875"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV45759.1"
                     /translation="MKRIAVIGGGASGLIAACFAAERGSVVVFEKQKKIGRKILVSGN
                     GRCNLSNRVIDAARYHGKNPKFVNNVFARFGPDDTLAFFLSIGIPVVELERGRLYPAS
                     LQSSSVVKVFEYELARRGVDVRLHRKIESIKRGNDGFRLTTAGKETHDFDAVILAAGS
                     CAYAPAGASSIGYELAASTGHTVREPFPAILPINIPNKALHRLEGIKWDVGAALEANG
                     RTVARSAGELLFTKYGISGPAALDISRAANENVLARRSPVITIDLFPERSRDELRATL
                     ESLWSDANKPIAFSLAGIVKERMPEVLCAIAGVDPEKRVAQTGAAERDRIASLMKDLR
                     LQPGEPRPFAEAVVAAGGVPVDEIDPATMESRITKGLYITGELLDIDGDTGGFNLQFA
                     WSTGAIVGTAV"
     gene            12246..12488
                     /locus_tag="PKJ16_01880"
     CDS             12246..12488
                     /locus_tag="PKJ16_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HNV45760.1"
                     /translation="MRVTAKDLRTHSRILLEAVARGEEVIITFRGRPRAKLVPITRTK
                     KKADDASDVFGMWRDNPAASDVNGYVRSKRKGRYAC"
     gene            12482..12862
                     /locus_tag="PKJ16_01885"
     CDS             12482..12862
                     /locus_tag="PKJ16_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019611996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV45761.1"
                     /translation="MLIDTDVLIWYLRGNQKAFDFIERQNGFTVSVITYMELVQGMRD
                     KQELNRFRKRFLEWNASIMHLSEDISTRALYIVEKFYLSHSLEMADALIAATALVTGL
                     PLATCNDKHFRSITGIELVPLKIS"
     gene            12859..13224
                     /locus_tag="PKJ16_01890"
     CDS             12859..13224
                     /locus_tag="PKJ16_01890"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNV45762.1"
                     /translation="MKNAILKDHFLHPRNMGALERATHRAMVKSDTCSDIVRMSARIE
                     DGVVREIRTEVYGCGYSIAAASLFTESVTGKRAPEIPEHYRGIIAPLTSDVPPHNENC
                     LLLPLRTFMALRPSADMEE"
     gene            13226..14008
                     /gene="fdhD"
                     /locus_tag="PKJ16_01895"
     CDS             13226..14008
                     /gene="fdhD"
                     /locus_tag="PKJ16_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HNV45763.1"
                     /translation="MALPAQVTIRAWEFGPEAAAEIEVAVSAEYHFTIAINGNPIVRI
                     ACSGSDLEELAIGHLVTEGIVRAPDDVRGCAVREAERIIDIATSDDEALLDRLFAVRS
                     IASGCGQASEGALPFERPVAGNRTRIAANVITASMREFLHASDLHRATRGVHSAALYT
                     TGGERIVFYDEIGRHNAIDKLAGYALMHNHDVSNTMLLSTGRLSSEIVGKAVRAGVPV
                     VASKASPTSLGVELARASGLTLIGKVRGERFCAFSGIEGISE"
     gene            complement(14037..15167)
                     /locus_tag="PKJ16_01900"
     CDS             complement(14037..15167)
                     /locus_tag="PKJ16_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45764.1"
                     /translation="MVTVTGPRQSGKTTLVKDTFPGHAYVSLEDPDTREMAALDPREF
                     LRKRGKGMILDEIQRMPELLSYLQGIVDQSDRAGRYILTGSHQFDLFSAVTQSLAGRT
                     AILKLLPFSIHELDSINADFSVDEYLLHGFYPRIHDRGLDPVAAHRNYFETYLQRDLR
                     QLIAVKDLRLFQKFVRLCAGRIGQIFNASGLSNDVGVSVPTVNAWISILEASFILFLL
                     EPYYENSGKRLIKSPKLYFYDVGLAAYLLGIESIKHVERDPLRGNLFENMVIAELVKR
                     RFNQGREHRLSYYRDSNGNEVDVLYRDGKDVIPIEIKASSTFSGDFLKGLRYLRRQFP
                     ENTPRSYLVYAGDEAEALDDIQLVNYKNAASILPTAGRTRGR"
     gene            complement(15296..15568)
                     /locus_tag="PKJ16_01905"
     CDS             complement(15296..15568)
                     /locus_tag="PKJ16_01905"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HNV45765.1"
                     /translation="MTIADDAAGRLPPDFPTEVTFKAVFRSSPFTRDTIRSLVLEHNV
                     DPEISQRESREGRFVSITVTAVFPSEKVLQAVCANIAALDSFVTMF"
     gene            15880..17322
                     /locus_tag="PKJ16_01910"
     CDS             15880..17322
                     /locus_tag="PKJ16_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HNV45766.1"
                     /translation="MPHTNLQEFIRALEAAGDLRRIAAAVDPRLEITEIADRVSKAGG
                     PALLFENVTGSSFPLLINAFGSFPRMERALNCASFDEIARKIETLVKVQPPQGIMDKV
                     RLLFTLKDIAGIMPKKVKSAPCQANVIRDERLLDHLPILTCWPGDGGPFVTLPIVITK
                     DPDTGIQNAGMYRMQKFDARSTGMHWQYNKDGARHYEKFKALGKRMEVAVALGGSPAV
                     TYAASAPLPPDVDEMLFAGFLEGRAVELVKGKTVDLYVPAESDFVIEGFVDPGDETIE
                     GPFGDHTGFYSPADTYPVFRITCITHRDGAVYPATIVGKPPMEDCYMAKATERIFLPF
                     IKLIVPEISDINLPMEGVFHNCAFVSIKKHYPGQAKKVISALWGLGQMASTKFIAVFD
                     DDIDLADYSTVAWKLLNNVDPRRDLVFSEGPLDALDHSAPHANFGGKMGIDATRKTRE
                     EGMGRDWPPEIRMADDIRDLVDKRWREYGL"
     gene            17312..18157
                     /locus_tag="PKJ16_01915"
     CDS             17312..18157
                     /locus_tag="PKJ16_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HNV45767.1"
                     /translation="MDFRKFANLIMIEQTLFALPFAYIGIVFAGGGSVWTWIWATVAV
                     VAARTAGMAFNRYFDAEIDAKNPRTKNRALPAGEVSKTEVLSIAVASSFFLIVAAYLL
                     NTLCFYLSFLAVGMLATYSLFKRFSSSSHLYLGLIEAAAPVGGYLAVSGDFQLMAFVL
                     GGAIMAWIAGLDIVYAIQDRSFDMDQKLFSVPVRFGVERAKLISAGLYVVSLGALVAA
                     GLLAGKGVVYWLTVICVGLVFIKQQLLARQQDIEEAVRHIFQLNNFVSPVLLAGTVLD
                     LFIKL"
     gene            complement(18161..18475)
                     /locus_tag="PKJ16_01920"
     CDS             complement(18161..18475)
                     /locus_tag="PKJ16_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013700296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HNV45768.1"
                     /translation="MKILSHSDIAYLKKKAHHLKAVVQIGKLGLTDALAAAADEALEA
                     HELIKVKFVGCKEERHEIAAACAERTGGALVGIIGNVAIMYRPRKDPAKRTIKLPEAG
                     WT"
     gene            complement(18690..19649)
                     /locus_tag="PKJ16_01925"
     CDS             complement(18690..19649)
                     /locus_tag="PKJ16_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45769.1"
                     /translation="MPPKKWSWNDESRERLDQVYAVLDEFEEFLPLTLRQIYYRLVAQ
                     RRVENSRAAFGAVASICKHARVEGLISWADIDDRPGGFYDLSGCYDTRVFVEESLKAL
                     MSGYYRNLMQTQDAYLELWVDAPSMRTFFSAIARRYSVSMALCGGFSSVSFLNDFMNR
                     AFDAVREGRRPVILFFGNYAPGGTDLLESMKATFAKEESLSNISFKRIALGRDDVTRH
                     RLPANPDAIRGAANGGRRRAARFGELPVELDALPPDLLERMIREAIEAEIDLALFRSE
                     QERQRADRETIERLRARISGFLDNNVSLVTAAEIEYIPAKNGK"
     gene            19755..20357
                     /locus_tag="PKJ16_01930"
     CDS             19755..20357
                     /locus_tag="PKJ16_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011339990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HNV45770.1"
                     /translation="MHVVAITGASGAIIGIRLVEELLRSGARTAVVVSDTARAVIRHE
                     VLRDADYATMRALLERRGRAVPGDALREFDPGDLFAPIASGSFHFASITVAPCSMKTL
                     SAVAHGHAEGLINRAVDVALKERRRCVLVPRETPVSEIHLENMLRARRAGADILLPVP
                     AFYHHPRSLDDVVDFIVGRALSLVGIEHALFTQWGDDASH"
     gene            20341..21456
                     /locus_tag="PKJ16_01935"
     CDS             20341..21456
                     /locus_tag="PKJ16_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNV45771.1"
                     /translation="MMLRIDAMLDPRLRPVAEKALAGEPASTEDARAMLETDDIVGLG
                     AIANHHRRRLHGDRAYYGVNMNLNYTNVCELRCPLCAYSKDAGDEGAYRYTLDEIESR
                     VRDAAERGIDEVHIVGGLHPELKLDYFEEMLRRIKRIRRELFIVAFTAVEYDYFARIN
                     GLALEEVFRRLIDAGLGALPGGGAEIFAPETRRVIAPKKMSGERWLEVMETAHRMGLK
                     TNATMLYNHIETTDQIVDHLTRIRALQEKTAGFKTFVPLPFHAENTAVRTARPPRTGF
                     GDVRIYATARVFLHTVPHLKALWMYLGERLAQTMLAFGADDIGATYHHERVVHAAGAT
                     SAASGSEARLRRLIERAGFVPVRANAAYETIDPEARA"
     gene            21453..22262
                     /locus_tag="PKJ16_01940"
     CDS             21453..22262
                     /locus_tag="PKJ16_01940"
                     /inference="COORDINATES: protein motif:HMM:NF014658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HNV45772.1"
                     /translation="MRLGCIDYLNCFPFYYHMLEKQPLPGVEIVSAYPSALNGMMNEG
                     ALDMGPMSAAEYADIRERTLLLPQFALSSVGYVKSVVLLSNHPIEELSGKTVGLSNAS
                     KTSVVLLKILLQKHYGLAPRYLPAPPRPDLEALDAALVIGNEAMMDDRQPVPFSYDLG
                     DLWLRRTGHPVVFAVFAVQREYARAHDHEVRAVIDSYRASLDRLASERERLIVAAARR
                     YPDIRYDIDHYYTLLQFEFTDALKNALQFYFDEAAALSLLPRVPSLVFFPH"
     gene            22447..25983
                     /gene="nifJ"
                     /locus_tag="PKJ16_01945"
     CDS             22447..25983
                     /gene="nifJ"
                     /locus_tag="PKJ16_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010527917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HNV45773.1"
                     /translation="MDGNTAAAHVAYAFTEVAAIYPITPSSTMAECVDEWAAYGRRNI
                     FGETVRVVAMQSEAGAAGAVHGSLQAGALTTTFTASQGLLLMIPNMYKISGELLPCVI
                     HVSARSLSTHALSIFGDHSDVYAARQTGFAMLATGSVQQVMDLAGVAHLAAIASRVPF
                     LHFFDGFRTSHEIQRIEVLDYDTLDALVDREALAAFRNRALSPETPVTRGTAMNPDIY
                     FQLREAANTYYDALPDIVERYMAEIGRITGREYAPFTYYGDPDAERVIVAMGSVTETV
                     RETIDYLRAHGEKAGLVTVHLYRPFSARHFFARLPHTVTRIAVLDRTKEPGAPGEPLF
                     MDVATAFIGRASAPVIVGGRYGLSSKDVTPQHIMAVFEHLTEASPRHGFTVGITDDVT
                     NTSLRLPPEPDIFPAETFKARFYGVGSDGTVGANKNSIGIIGDSADLNAQAYFAYDSK
                     KSGGVTVSHLRFGEKPITATYLVTRPNFVACHVPAYLGQYDMLRGLKPGGTFLLNAPW
                     DDEETKRHLPASVKRYLAHNGIRFYAIDASRIAEEVGLPGRTNSIMQAAFFRVAGVIP
                     HDEAAHRMKEAVKKSYGKKGDDIVEINCAAIDRGAEMVREIAIPPEWKDAPDDAADAA
                     CGNSFVDTIARPINRLEGNDLPVSAFTSMPDGTLPQGTATYEKRGIATHVPEWIPSTC
                     IQCNQCSYVCPHSVIRPFLLDDGDVAALPFPLDLLPAKGKGLEGHRYRIQISPLDCYG
                     CGNCAEVCPVKQKALVMRPFESQLAQRPIWDHLAAKVSYKDHLMAKTTVKGSQFAKPL
                     LEFHGACAGCGETPHYKLITQLFGDRMMIANATGCSSIYCGSAPSTPFTTDARGRGPA
                     WASSLFEDNAEYGYGMHLGYSKLRARAARLIETLAAGARDGALRDASAQWLRVMDDAS
                     ACDAATDRLVERVRGSTDETSAEVDRLRAYLRKRSIWMVGGDGWAYDIGFGGLDHVIA
                     MGEDVNMLVLDTEVYSNTGGQSSKATPTAAVAKFAASGKPLGKKDLGLMAITYGYAYV
                     AQVSMGANQAQFLRAVLEAEAYPGPSLIIAYSPCINHGIRSGMETTQRVMKNAVACGY
                     WHLYRYNPLLEREGKNPFVLDSPEPDWGAFREFLMNETRYTSLQHEFPEHADGLFAAA
                     EDDARRRYNTYRRLADSMAASTDVPEASNERA"
     gene            25970..26974
                     /locus_tag="PKJ16_01950"
     CDS             25970..26974
                     /locus_tag="PKJ16_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV45774.1"
                     /translation="MSARKFSIVVPALFILGGVFLVPFGTKPSTGQTAKKAPTKQTYA
                     YPSFYKGFYLTSGSGNNRAKLERHITRAKKAGLNAVVIDVQTPGMRVAVTPAEHVAYC
                     RAMGFHPIARVVVFPEGLSEYPAAAELIENRLAVAEAACRAGYREIQFDYIRFNDHRV
                     NRALSLAERYEYITGFLKKARERLSKYDVRIAADIFGRIPLNADDKIGQKMELLDGLV
                     DVICPMAYPSHYTWSKKMMADPYYTVHITSKRALERAPRSAIVTWIQAFRMKVKRSKL
                     GYDRYVAAQIKAVHDSGAGGYLLWNAAQDYAIPLAVAGEINRRRARVASLSETPSGGN
                     "
     gene            26976..28019
                     /locus_tag="PKJ16_01955"
     CDS             26976..28019
                     /locus_tag="PKJ16_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013684198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HNV45775.1"
                     /translation="MGSVKDLTVINAPSETQAGLGRFAFSDRYSVFDWGEMPDHIDGK
                     GAALCVIGAHFFELLAREGVPVHYRGLFDGDGRIKRLGELTAPSCDMAVSLYRVLRPA
                     ERDGSYDYARYADVNNNYLVPLEVIYRNRLPEGASVFARLREGTVTLAELGLTEMPAP
                     GAILDRPILDVSTKLEVTDRYITWPEAQAISGLLDGEMDTLRGLTLRINETITREAER
                     IGLANEDGKIEFAIDEHARLTVVDVLGTPDECRFTWNGLHVSKELARVFYRGSAWHAA
                     VERAKREDRINWKSRVDGAPAPLPAPLARNLSHMYQAVANGLTGRDFFPVPPLAEVMK
                     QVALELGALRREG"
     gene            28021..28329
                     /locus_tag="PKJ16_01960"
     CDS             28021..28329
                     /locus_tag="PKJ16_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNV45776.1"
                     /translation="MADVMIHEFLLGFIKIYILQFADKEPVYGKEIHDQLEEFGFKIS
                     FGTLYNTFHGFEEKGYLVHEERNVNGKIRKYYRITKHGREALATAKGKARELFTALYE
                     "
     gene            28362..29576
                     /locus_tag="PKJ16_01965"
     CDS             28362..29576
                     /locus_tag="PKJ16_01965"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV45777.1"
                     /translation="MKLSQRIKNVKPSATLALTALANKLKAQGVDVIGFGAGEPDFDT
                     PEPIKEAARRAINTGKTKYTPVGGIPELKAAVCEKFKRDNALSYKPAEVTVNCGGKHS
                     FYNLMQSILDEGDEVIVPAPYWVSYPPIIALAGGRPVIVDCPGERGFKMTPEQLSEHV
                     TDNTRAIIINSPSNPTGSAYTRAELAALAGAIAGRDIIIVSDDIYESMLYDARTFCTM
                     ANISDELKARTIVLNGVSKAYSMTGWRIGYMAGDAAIIQAVETLQSQSTSNPTSISQW
                     AAVEALTGDQSFIGEMMTAFTRRRSLIVDGLNRAPGISCQPPDGAFYAFPRVSGVFAL
                     PGWPAVRERYAGQANSSALCSHLLDEARVAVVPGVEFGNDEYLRLSFATSDENITRGV
                     ERICEAMQRLAG"
     gene            29692..31026
                     /locus_tag="PKJ16_01970"
     CDS             29692..31026
                     /locus_tag="PKJ16_01970"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV45778.1"
                     /translation="MVGCSYGRFFQVTVAGGSYQEGLVSHMQGVPSGIHLEEKDIYAD
                     LLQRKPGQGELSSPRREPDIPVIVTGVNAADTMPGFANAEHTNGTPLTVLIPNLDRHF
                     EHIDQYQRTNRTPRPGHASYATYQKYGSFDDSIGAGIFSGRYTSTIVAAGYVAKRILR
                     DHGIEVFSYVKEAAGVAMPEMPYDAIKERVRIFREFRRDNDPLYAIVYAQGRIKPGMR
                     MLEKMAVLAEIERMVPDITREPLSDAQVAALAEQGVHPVLNCPHLEAATEMYNKILEI
                     RDGGDSSGGLVEVVATGVPAGMGEPVFEKLDGELGRMMSIGTIKAVEIGAGMRVKDMT
                     GSECNDQMRARDGRVEFMSNSAGGITGGISTGQDIVARLAVKPTPTIAKPQKTIDKVS
                     NTDAELAAVTRRDPTIVSRVWPVAEAFTAIVLLDQLTLHIAYQALRSHYKER"
     gene            31034..>31878
                     /locus_tag="PKJ16_01975"
     CDS             31034..>31878
                     /locus_tag="PKJ16_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNV45779.1"
                     /translation="MERAILEYKGARYELPVIDGTEQERAIDISNLRKDSGLITLDVG
                     YQNTGSCTSAITFIDGDRGILRYRGIPIEVLAERSTFTETAFLLIYGHLPVEAERRAF
                     SERLTENSMIHEDMLRFFDGFPPTAHPMAILSTMVNALSVYYPGYFTEDFESGTFDLM
                     AVRLISKIRTIAAYSFKHSIGEPFVYPRHDLRYCANFLSMMFHSPVKPYEIDPDIEKA
                     LNALLILHADHEQNCSTSTVRLVGSSMVNLYASICAGICALWGPLHGGANQHVIEMLE
                     GIQQQG"
BASE COUNT         6009 a        10418 c         9787 g         5564 t
ORIGIN      
        1 gcgttttcgc gaagccgtag cacaggcaca cgaccaccat gagcggttcg tcctcatggc
       61 cgaggcggcg ctcctggtgg tcgtgtgcct gtgctacggc ttcgcgaaaa cgcttctccc
      121 cgagaccccc gcgctcatcg tcgccgggac ctgcttcgtg ttggaccagc tcctcatgtg
      181 ggcggggatg gcgcgcgcca cctggctcaa gaagatcgcg cgcgcgcccg aggacgtctc
      241 gcccaccctc gccatggggg tttccatcga ccatattttt tcaatcgcca tcgcgctcgc
      301 aagcggcgtg ctgtggctca cgctgggcta ccagtatgtc ttcttgctgg gcgcggcgat
      361 cgcgctggta aacctcgtgt cggcatcgta tgtccgcgtg ccgtcgcgaa gcgcctgacg
      421 cggcatcgac agcgaatcaa cacgaatgtg agaggagaat catgaagatc atcgccctca
      481 gcgacctgca cggaaacctg gaccgcctcg acgcgatcgc ggacgagctg cgcgccgcgg
      541 acctgctgct cgtctccgga gacatcacga aaagcggaag cgccgatgag gccgccggca
      601 tcatccgcac gctccaagag tacaacgccg ccctgctcgc ggtgcacggc aaccgcgatt
      661 tccccgaggt ggcgaccgcg ctcgacgagg cgggcgtggg gcttcacgcg cgcgggacga
      721 tcgtcgacgg gatcggattc ttcggcgcgg ggggcgggcg caccacgcgc tttcatacgg
      781 cctgcgagta cagcgagggg gagatcctgg agtttctcca gcgcgggttc gcagccgtcg
      841 ccggggcgcg ccgggtcgtg ctgctctcgc acatgccgcc gaagggcgtg cgcgaccgca
      901 tgttcctggg attccgcgcg gggagcgccg ttgtgcgcga cttcatctcc ggcaaccgcg
      961 tggatctgtg cgtgtgcggc cacatccacg aggcgcgcgg gaccgagcgg ctcatcgaca
     1021 cgatggtggc gaatgcgggg tccttccgca acggatggta cgcgcgcatc gagctggacc
     1081 ggacggtcac ggtaacgatc gaacgggcct gagcgtccga cgctattccg ccgcgcgctc
     1141 gtcccgcacg agccaggcga tcactcccag catgaccagc atgcacgcgg ctgtcgcaag
     1201 gaaggtgaag cgcatcccgc cgaaatgata gaagagcgcg agcaggaccg gccctcccgt
     1261 ctgccccgcg cgaaggagca tcccattgaa cgacatgaag gcggcgcgct gcgcgggcgg
     1321 cgccgcaccc gcgaggagcg cctgcaggga gggcatgacg atgccgttgg ccatcccgcc
     1381 gatcaccacc ggaacgagca tgagcggata gctcgcggcg aagggtatca gcgcgcacac
     1441 gaccgcatag agggaaaaac cggcgatgac gaaggcccgc tcgctccaac gccgcgcaag
     1501 gcgcccgagc tgagaggagg tgatggcgtt ggcgacggac atcgccgtga ggaacaggcc
     1561 gatcgccagc gccgtcgccc cgaacgactg cgccatgagg atggggaagt aggtgaggat
     1621 ggccccgtag ataaagatga acgccgcgag gcttgcaatg aacaacccca ccacctgccg
     1681 cgcccggact acgcggaaca ccgcggcgaa gtacgcgcgg atttcgggac gggcgtgcgg
     1741 ctcgggattg ttgagaagaa agaggaccgc gagcgcgacc gggatggcgg cggccgtcat
     1801 gaggaaggga tagttccagc ccagcatggc cagcgcgcca ccgatggccg ggtagctcgc
     1861 ggtgccgatg ctgagcacgc tcgcattgta tcccagcgcc gccgtgcgct cgcgccccga
     1921 gaacaggtca ctgatgatgg tcacgttgac cgcgcccagc gaggccgcgc cgatcccctg
     1981 cacgaagcgc agcgcgagga gcgacgcgaa gctttcgcac cagaagcaca tcgtcccggc
     2041 gatcccgaag agcaccagcg agggaacgag gacccgcttt cgcccgtacc ggtcggcgag
     2101 gaacccgaat accggcgtaa ggaaaacccc gggcagggtg aacaccgaga tgatgaggcc
     2161 gatctcgcga tcggcgatcg agagcgcctt cgccatcgcg gggatggcgg gggtgatgtt
     2221 cgagacgccc atcaccacca tgagggtcac ccagaagacg atctggaggt tggcgtccaa
     2281 atagagcttt cgcggtctgt tcacgttccg gtcatcctcg cgtgggcggg cgtatcgatt
     2341 cggcgcgaac cgtcttaggg tcccgacgcg catcctgcaa ccatttatta tttgacagga
     2401 cggacgggca tgcggcacgc tcggatccaa ttccgtctct gttttggaat tgttccgatc
     2461 cgatgcgcgc ggaatgcatc ggacatatcc ccatcgaata cacggtacgc cgcccatgga
     2521 actcagccga cccgtcgtga aactgctggc cgtcacgccc aacgccgagg ccctcatcga
     2581 ggaggccggg cgcacctgct acctgtcatt cgacaagatg accgacgaga gcggcgcggg
     2641 cttcatccgg ggcgcgatcc gacgcggaca cctctccatc atcgagcacg cagcggccac
     2701 cttccggatc ctcggcgcat cgcgcgcctt cacgcaccag ctggtgcgcc accgcctcgc
     2761 aagcttctcg cagcagtcgc agcgctacgt gagcgaggac gaattcaatt atatcgtccc
     2821 gcccgagatc gcggcgaacg acggggcggc gcgcgtctac cgaggattca tcgaacaggc
     2881 gcaggcggcc taccgggaac tgcgcgcgct gggcgtcaag aaggaggatg cgcgcttcgt
     2941 ccttccgaac gcgctcgaga gccagatcgt cttcacggcg aatttccgcg agctgcggtt
     3001 catcttttcg cttcgattgc aaaagagcgc gcagtgggag ttccgccggg tgtgcttcga
     3061 catgctcgcg atcatgcagc gcgaggcgcc ggcggtgttc ggtgatttcg ttatggacga
     3121 ggcggacctg acggcggcgt cgaatctgac gtaatcagga cacgcggaac ttcgcgatct
     3181 gcgcctggag gccctgggcg ctgtcgttgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnt cagcagcatc agaaacggca acagcgtgat cacgatgatc gacgaggtca
     3361 gccgcggaaa gatgttcatg tgcaaattga gacgctcccg gggccaggcc ggaaagaggc
     3421 ccagccgcat gagcttctgc gtgtacagct ctgtgagaat aaaatacagc accgtaccca
     3481 gcggggcgtt gatgacgatc gtcatccaaa ggttgaacgt ttgctcgctg ttcgtattgc
     3541 cgaaatatac cgtcggcagc acccccatcc ccagtccgaa cagccaccgg aagaacgccc
     3601 caaagctgtg aagaaacggg agcatgatga agcggcggtg cgcgcgatta tactcgtcct
     3661 ccggaacctc cgcgccggaa agcagcttgc gaaagtatcg gagcaccggg gcgatgacga
     3721 tgaaattatt aatttgcgtc gtgatgaacg atatgggaaa cgccacggcg acggaaacga
     3781 gaaaaatcac aagctggtcg tgggtgagat tgacgttcga ccacacgtaa aaaacgatca
     3841 tcggcacgat gaaaaaatag ctgatgccct ccgtgcgcag gatgaactgc agggggaagg
     3901 tccgcgccgc gagccgctgt ttgtcggtca actgcatggg catattcgat gatttttctc
     3961 tctatatgta tcggcagcac cctcaggggc ggcgaatgat ttgtcaatgg aaaagcgtcc
     4021 ggatgtgctg tttgctaaaa aaaactgaac aattttaaaa tatttgactc agggtttaat
     4081 atttacaccg ccacgcatcg gatatccaag cgagcttccc ttccggcttt gctgcgggct
     4141 tgtgtttcaa ttggcaagca cacgaagcgc agaggatcgt gccgcttcgc gccactcaca
     4201 ttggcaggtg cgtatgtgtc gctgtaaccc gatccgttct tttcaggaca cgcggaactt
     4261 cgcgatctgc gcctggaggc cctgggcgct gtcgttgagc gacttcgcgg agtggctcac
     4321 gaactcggcg ctctcggcgg tatgctgcga gagctcgttg atggagttga ccgcgcgggt
     4381 cagctccgcg gtggtcgtct gctgctcggc cgaagcgaga tagatgtcgt tggccagcga
     4441 attcatgcgg tccaccttcc ccttgatctc atcgttcatc cggatgagct cctgcgtgaa
     4501 atcgaacacc tgcccaatca ccgcatcgat gtcgaggatg ctcgccttca tgctgttgaa
     4561 cgcgtccacc gtgttgttga gcgaggccag ctcggtgttg atcttggcgg agttctcgga
     4621 aacgctcttc ttgatgtttc ccgccaactt cgtcgcctgg tcggcaagcg tgttcacctc
     4681 gtcggccacc accgaaaagc cgcgaccggc ctcccccgcg cgcgcagcct cgatcgccgc
     4741 gttgagcgcc aacaggttga tctggtccgc gatgtcgttg atctgaccgg ccatctcgtc
     4801 gatgttctgc atgtagccga ccatgttgcc gatcgtcttc accgagcggt tcatcagacc
     4861 ctcgccgtcc tcggcgatct tcaccgactt ccccgcgcgc tccgacagcg tccacgtctg
     4921 cttcgccagc gcctcgccct tctcgctgat gcggctgatg tcgtccttca ccgcaccggc
     4981 gctgtcgagc tggctcttca cgttgctcag gttcatctcg aaggaggccg acatctcctc
     5041 gaacgaggag ctcgcctcct cgatgatggc cgcctcgtcg cgcgccatgt ccgaaagcgc
     5101 attggacgat tggatgagct tctcgctgga ggcgctcagc tcgcgcgcgg tctccgcaac
     5161 ggcgtggacc atcgcgcgca ggttctcctt catgccgtac accgacttgt tgatctgcgt
     5221 gagctcgtcg agcaccgcga tcttctgcgc gggtgcgaag agatcgccct cgcccacctg
     5281 cttcagaaaa ccctggatga tgcgcacctt gaacacgatg ctcttggtga gaacgtacga
     5341 caccaggacc gcgccaatca cgccgatccc gccgatgatg ccgatcttca catacgccag
     5401 cgtcttatcc acctggagat cggcgatgaa gatgatgaaa aacgtcagca gcatcagaaa
     5461 cggcaacagc gtgatcacga tgatcgacga ggtcagccgt ggaaagatgt tcatgtgcaa
     5521 attgagacgc tcccggggcc aggccggaaa gagaccgaga cgcatcagct tctgcacgta
     5581 cagctccgtc aggataaaat acagcaccgt gcccagcggg gcgttgatga gaacgcacat
     5641 ccagagattg aacgactgca cgctgttgat atccgcgaag atcatggtgg gcacgataac
     5701 gaaggaaaga ccgcccaccc atcggaaaaa cgcgccgaag ctgtgcacga agggcaggag
     5761 cagaaaacgg cggtgcgcgc gattatactc atgctcggag acatccgccc ccttgaccag
     5821 attcctgaaa tagcgcacca ccggcgcaat cacgatgaga ttgttgatct gcgtcgtggt
     5881 gaatgacacg atgaatgttc cggcggcaaa catgagaaaa agaatcatct ggtcgcgggt
     5941 aaagttcaga ttggaccaca cataaaacgt gagcatgggc acgatgaaaa agtagctgat
     6001 ggcctcagtg cgcaggatga actgcagggg aaaggtcctc gccgcgagcc gctgtttgtc
     6061 ggtcaactgc atgggtatat gtatcgacgc gccgtcaact caatgtcatc gcgagcgaag
     6121 cgatctgcat ggcaaaaccc gttgtgcaac aattccacta gttatttctt tatatctttt
     6181 tcatcggcat tatccttccg atcaacaaac gttttgtcaa cagaaatgcg tccgtttgta
     6241 tcattaatat aattacacaa aacctagaaa atattgactg agaggaaaca atcagaccac
     6301 ctcgcaccac gcgttcatga gcacgtcctt tccggcatcg ttgcgcgcca ggagtccgat
     6361 ttttccctcc gcatcttcca tgcggaaccc tttaaatgtg agcgtctgtc cgggaagcac
     6421 cgcgcgtgcg aactgcacct tgagtttccg cacccgcaac gggtcgcgct ccgggcccgc
     6481 gcaggtatcg acaaccgcct tgtgcgcgaa ggccatggtg cacaggccct gcaggatgac
     6541 gccgtcgagt cccacgccct tcgccgcggc gtcgtcgagg tgaatgggat tgttgtcgcc
     6601 cgatgcctcg gcgtagatga acgtctgccc gttgcgaacc gtcatcgtcc gctcccagag
     6661 gaactcggcg gggcgctgcg ggcgctcgcg cggatcggac acgctccggt cgccggcgga
     6721 caaatcgaag aactcccact tcgttttcac gacctcctcg tcgcgctggt tccgcgagac
     6781 ggtctcccac cccaggagcc ccccgaagcg gcgcacggtc acgtgcgtga tcacggcctc
     6841 ggtgcggatc acgtcgccgg gacgcaccgg gcgcagccac tcgtactcct gcgaccagtg
     6901 caccaccttc tgcatgttca tgccggtttc gggatcgttc atgatgttca cgatgggcct
     6961 gccggcgtaa tagaccgcgt acatgggcgg cgcgatgatc ccgccctcgc ggcggccgtc
     7021 gaaataggcg tcgttgtcct cgttgtacgc gagcgcatag aagatgcttc cgtgcgcggt
     7081 cacctcggtc ggctcggccg tgtacgattt tccgacgata tccttgttga gcggcatgcg
     7141 ttcctcctct gtgtgtgtac gaatcgatgt gtcgcataac ttgtcgtcgc tgcgcgcgta
     7201 gcggcgtcat ccgtatgatc cgcgcgcgca ctgccgccga tcaacagccg ccatgccaca
     7261 tgcacgcccg gcacgatatc gccgtctcca cgcgcaacac cggtgggcgg catccccttc
     7321 ggatgcggcg ccccgcagcg cgaagcgcaa tcaccgcgcc ccgcgatgca cattcgctcg
     7381 cgcccccggt tcggtccgcg gcgcagcagg acggtacacc ctgcgggaat tatttcaatt
     7441 gttttttaat gccagatccc gccggagcgc cagcgtggcg cgcgggtgac cggcgggtag
     7501 cgctccggca gcaccgtcgc gtcgcgcaca tgctcgatat gccgctgtgt ctcctcggcc
     7561 agctcgagca ggcgcgcgat tctcttctcc gtttccggat gcgtgcgcag cgcggagggg
     7621 cccgggacct tgcgccccgg catgaggatc atgtcccaga ggcgcatcgg gtagcgctcg
     7681 atttttccga gggcggaggc gagcccgcgc ggatcgccgg tgagcgcgac cgctcccagg
     7741 tcggcctcga actcgcgggt gcgcgagagc gcgagttgca ggagcaccgc gagcgtgggc
     7801 gcgcccatca gcaccgcgag cgccggcagc ggcaccatca cccccgccgc cagatacagc
     7861 ggcaacgaca cgacgaggac gaagatcccg accgtcgaaa acacattggt gaaacggctc
     7921 aatgaatcgg ccaggttcat gatccacaga tccccgtggc gcacgtggct caattcatgc
     7981 gccaggacgc ccgcgagctc ccgcgggttg agggcgcgga tgaggccgtc ggtgagggcg
     8041 atccccgcgt tcgtgcggtc gcccaccgaa aaggcgttca tgatgctgct ggggacatag
     8101 tagaggacgg gtgccgcggg cagatcggcc cggcgcgcga gctcctcgat gacgcgatag
     8161 agatacggcg cctcgtactc ggacagcgcg cgcgcccggt acattttgag caccatgcgc
     8221 ggcgacaccg cgggactcat cgccatgatg gccagcgcga acacgagggc cacgacgagc
     8281 ccgaagggcc cgctcatgat atacccgatc gcccccagca gggccgccat gagcgcgaac
     8341 aggagcgcgg tctgcaacgc attgcgcacc ttgcgccgat agtatttcgc gggcggtatc
     8401 atagggggtc ggccactcgc tacttcatgc gtaatttccg cttcaccgcc ttcaccggat
     8461 gcaacctgat gtaatcacgg cattccatga taaccgcccg atgcaccgct taatgcttga
     8521 aggcccgctg gccggtgtag accatcgcga ccttggggtc cgcctcgttg caggccacga
     8581 tcgattcgaa gtcgcgctcg gagccgcccg gctgcacgat ggccgtgatc ccctgccgca
     8641 ggcccacgtc caccccgtcg cggaagggga agaacgcgtc cgagaccatc accgatccca
     8701 cgagcccacc cttcgcgtcc tccgtctcgt cgtcgatgtc gtccagctcg gactcgtcgc
     8761 gctcgccctt ctccaccgca agcgcgagct cgttgtacga aatgccgtgc cggtcgaagc
     8821 agatgaggtc ggcgaatttc gcgtaggcct tgtacaccgc gatgtcggcc acgcccacgc
     8881 ggtcctgctc gccggtgccg atccccacgg tgcagccgtc cttgacgtac agcaccgaat
     8941 tcgacgtcac tccctgctcc acgaaccagc cgaagagcag gtcctcgtac tccttctcgg
     9001 tggggagccg gtcgatcgcg tacttctgcc ccttgtactc ggcggcggcc ggcgcgaaat
     9061 cgtccttcgt cttgacgcga ttcaccggcg actgctggac gatgatgccg ccgtcgatca
     9121 tcgacttgaa atcgacgaag cgccggtcgc ggtagcgctc gagctcggcg atgcgcgcga
     9181 tcttcacgat gcgcaggttc ttgcgctttt tgaggatgtc caccgcctcg ccggtgtaat
     9241 cgggcgcggc gatcacctcg atgtactgct tcgcgacgag ctcggcggtc tcgcggtcaa
     9301 cggcgctgtt gagcgcgaca cagccgccga aggccgcgat gcggtcggcg cgaaaggcgc
     9361 ggtcataggc ctccgcggcg gtcgatccgt acgcgacgcc gcagggattg ttgtgcttca
     9421 cgatcacgca ggcgggcttc gccatgagat atttaagcac attgagcgaa ttgtcgatgt
     9481 cggtgaggtt gatcttgccg ggatgcttgc ccgactggat catcatcgcc tcgtcaatcc
     9541 cgctcacgag ggcgttgccc gcgccgatga actcgcaccc gccgaggacg aggttcccgt
     9601 tcacgagctc gtacatcgcg gactcctggc cggggttctc gccgtagcgc aaccccttct
     9661 cgatgagcgc gcccccgtcc tcgatgcgcc acacgcgttt cttgtacacc agtttctgtc
     9721 ccccgaaatc gatggtcatg gtttcgggaa agtggtcgtc catcacggtc ttataggctt
     9781 ttttcaggtc ttccatcgtc cagactcctt tataacgtta tgtgcgatat tgtttctccc
     9841 aagacctcat cccccggacc cggcgagcgc tttccggcct cctgcctgcg cccgccactg
     9901 cgacatcctg ccgggagcct tctcccacag tgaaggggga ggtcgccacg agataattcc
     9961 cggcacgccc ctctcccttc gggagagggg ccgggggcga ggtcacgacc agcggacgcg
    10021 cttctcctcg gcctccttcc gaagctcgat gaaccgggcc atgaggtcgt tcatcaggtc
    10081 ctcgatccgt tcggggctgt aggcggcggt gggcagctcg gacccgatct taacatacac
    10141 ggtcgccgga tgcaccgcca gcgattcggg gcgcagaatg ccataggtcc cgttgatgaa
    10201 aatcggcacg atgggtacgc cggtcttcac gcacagatga aacagcccct tcttgaacgg
    10261 ttgcagctcg cccgaccgcg tgcgcgtccc ctcgggaaaa atggcgatcc ggtttccgcc
    10321 cttgatcttt tccgccgctt catccaggct cttgatcgcg ctcgcgcggt ttccgcggtc
    10381 gatggacacg tagccctgca gcttcatgtg ccagccgaga aacgggacaa agaacaggat
    10441 cttcttggac acccagccga acttcaccgg cagcaccgcg ctcaggacga agatgtcgaa
    10501 atggctcgca tggttcgatg caaagatctg gacccggtcg cggctcacat gctcaagccc
    10561 gtccacgtcc accttcaccc ggcagaacca gagcgtcgtt ttggaccacc agcgcatgca
    10621 gaaatgcgcc acgcgcccgc tcggactata cacgaccgac gtgaccgata cggaccccca
    10681 caaaatggta ttgatgacaa taaagatgat gcaggcgatt gaattgatca tctcgaacaa
    10741 tagattcatg tgatcaagcc cctcccctcg cggtacgatt ttaaaaaaaa attgtattgc
    10801 cgctgacgac ccggtgcggt acggataccc gaacgacgga cggacgcgat acggcatcgt
    10861 aaaaaacaga tacccttgtg catcctgccg taaatttcaa gaattatctc gtgcgggtgt
    10921 aaaataaaat gaaacggatc gcggtgatcg gaggcggggc gtcggggctc attgcggcgt
    10981 gcttcgccgc ggaacgcggc tcggtggtcg tcttcgagaa acagaagaag atcggccgca
    11041 agatcctcgt cagcggaaac gggcgctgca atctctccaa ccgcgtcatc gacgccgcgc
    11101 gctatcacgg gaaaaacccg aagttcgtga acaacgtctt cgcgcgcttc gggccggacg
    11161 acaccctcgc cttcttcctg tccatcggca tccccgtcgt ggagctcgaa cgggggcggc
    11221 tctatccggc gtcgctgcag tcgtccagcg tcgtcaaggt cttcgagtac gagctcgccc
    11281 gccgcggcgt cgacgtccgg ctccatcgga agattgaatc gatcaaacgc ggcaacgacg
    11341 gcttccgcct caccaccgcc ggaaaggaga cgcacgattt cgacgcggtg atcctcgcgg
    11401 cggggagctg cgcttacgcg ccggcgggcg cctcgtccat cggctatgag ctcgcggcat
    11461 cgacgggaca taccgtgcgg gagccctttc cggcgatcct ccccatcaac attcccaata
    11521 aagcgctcca ccgcctcgag gggatcaagt gggacgttgg cgcggccctc gaggcgaacg
    11581 gccggaccgt cgcgcgctcc gcgggcgagc tcctcttcac gaagtacggc atctccgggc
    11641 ccgcggcgct ggatatatcg cgcgcggcga acgagaacgt cctggcccgc cggagccccg
    11701 tcatcacgat cgacctgttc ccggagcgct cccgcgacga attgcgcgca acgctcgaat
    11761 cgctctggtc cgacgcgaac aagccgatcg ccttcagcct cgcgggcatt gtaaaggaac
    11821 gcatgcccga ggtcctctgc gccatcgccg gagtcgatcc cgaaaagcgg gtcgcacaga
    11881 ccggagccgc cgaacgcgac cggatcgcgt cgctcatgaa ggacctgcgc ctgcagccgg
    11941 gcgagccgcg ccccttcgcc gaggccgtgg tcgccgccgg cggcgtgccc gtagacgaga
    12001 tcgatcccgc cacaatggaa tcgcgcatca cgaagggcct ttacattacc ggcgagctcc
    12061 tcgacatcga cggcgacacg ggtggcttca acctgcagtt cgcctggtct acgggcgcga
    12121 tcgtcgggac ggcggtgtga ggggcgatgc gggatgcgat cggatcaacc acacgacgat
    12181 gaaaagtgtt gacgcggacc ggccaagtat atactttgaa ctatacactt gcaaggagcg
    12241 atccaatgcg cgttacggcg aaggacctgc ggactcactc gagaatcctc ctggaggcgg
    12301 tcgccagggg cgaagaggtg atcattacct ttcgaggacg gcccagggcg aaactggtac
    12361 ccattacccg aaccaaaaaa aaggccgatg acgcatcgga cgtcttcggc atgtggcgcg
    12421 ataaccccgc cgccagcgac gtaaacggct atgttcgctc caaacggaag ggcagatacg
    12481 cgtgctgatc gacaccgacg tgctcatctg gtacctgcgc ggaaaccaaa aggcctttga
    12541 ttttatcgaa cgccagaacg gctttacggt ttcggtgatt acctacatgg agcttgtcca
    12601 ggggatgcgt gacaaacagg aactgaatcg ctttcgcaaa cgttttctgg aatggaacgc
    12661 gagcatcatg cacctctccg aggacatatc gacccgggct ttgtacattg tcgaaaaatt
    12721 ctatctcagc cattcgctcg aaatggcgga cgccctcatc gctgcgactg ccctggtcac
    12781 cgggctgccg cttgcgacat gcaacgataa gcatttcagg tcaatcacgg gaatcgaact
    12841 ggtaccgttg aagatttcat gaaaaacgcc atcctcaagg accacttcct ccatccgcgc
    12901 aacatgggcg cgctcgaacg cgccacgcat cgcgccatgg tgaagagcga cacctgcagc
    12961 gacatcgtcc gcatgagcgc gcgcatcgag gacggcgtgg tgcgcgagat acggacggag
    13021 gtgtacggct gcggatactc catcgccgcc gcctcgctgt tcaccgaaag tgtcacgggg
    13081 aagcgggccc cggagattcc ggagcactac cggggcatca tcgccccgct cacttccgac
    13141 gtgccgcccc ataatgagaa ctgcctgctc ttgccgctga gaacgttcat ggcgctgcga
    13201 ccatcggcgg atatggagga gtagcatggc gcttcccgca caggtgacca tccgcgcctg
    13261 ggagttcggc cccgaagccg ccgccgagat cgaggtggcc gtgagcgcgg agtatcattt
    13321 caccatcgcc atcaacggca accccatcgt gcgcatcgcc tgctcgggat cggacctcga
    13381 ggagctcgcg atcgggcacc tcgtcaccga ggggatcgtc cgtgcacccg acgatgtccg
    13441 cggttgtgcg gtccgcgagg cggagcgcat catcgacatt gccacgagcg acgacgaggc
    13501 gctgctggac cgcctgttcg ccgtgcgctc gatcgcctcc ggctgcggtc aggcgtcgga
    13561 gggagcgctt cccttcgagc gccccgtcgc gggcaaccgg acgcgcatcg ccgcgaatgt
    13621 catcaccgcc agcatgcgcg agtttctgca cgcatcggac ctgcatcgcg ccacgcgcgg
    13681 cgtgcacagc gcggcgctct acacgaccgg cggcgagcgg atcgtcttct acgacgagat
    13741 cgggcggcat aacgccatcg acaagcttgc gggatacgcc ctcatgcaca accatgacgt
    13801 ttcgaacacc atgctcctgt ccacggggcg gctctccagc gagatcgtgg gcaaggcggt
    13861 gcgcgccggg gtgcccgtgg tcgcgtcgaa ggcatcgccc acgagcctgg gggtggagct
    13921 cgcgcgcgcc agcgggctta cgctcatcgg caaggtgcgc ggggagcgct tctgcgcgtt
    13981 cagcgggatc gaggggatat cagaataacc atccgcggac aaacgtgttt tatcgatcac
    14041 cgtccgcggg ttcgtcccgc cgtcggcaga atcgaagcgg catttttata gttcaccagc
    14101 tggatatcgt ccaacgcctc cgcttcatct cccgcgtaca cgagatatga ccggggggtg
    14161 ttttccggga attgcctgcg cagataccgc aaccccttca ggaaatctcc ggaaaaggtc
    14221 gatgacgctt taatctcgat ggggataaca tcctttccgt cccgatacaa tacatcgacc
    14281 tcgttgccgt tgctgtcccg gtaatatgaa agccggtgct ctcgtccctg attgaaccgc
    14341 cgcttgacca gctcggcaat caccatgttt tcgaagagat ttccgcgcag cggatcgcgc
    14401 tcaacgtgtt tgattgactc gattcccagc aggtacgcgg ccagaccaac atcataaaag
    14461 tacagtttcg gcgacttaat caggcgcttt ccactattct cgtaatacgg ctcgaggaga
    14521 aacaggatga atgatgcttc caggatcgat atccaggcat tgaccgtggg aaccgacacg
    14581 ccaacatcat tactcaggcc actcgcattg aatatctgcc cgatcctccc agcgcagagc
    14641 cgcacaaatt tctgaaataa gcggaggtcc ttcacggcta taagttgtcg caggtcgcgc
    14701 tgaagatatg tttcgaaata gttccgatgc gccgcaacag gatcgagccc gcgatcgtga
    14761 atgcgcggat agaagccatg caggagatac tcatcgacac tgaaatcggc gttgatcgaa
    14821 tcgagctcgt ggatgctgaa cggcaacagt ttcaagatgg cggtccggcc ggccagcgac
    14881 tgtgtcaccg cactgaagag gtcaaactga tggcttcccg tgaggatgta gcgcccggcg
    14941 cggtccgatt ggtcgacgat tccctgcagg tacgacagca actccggcat gcgctgaatc
    15001 tcatcgagaa tcatcccctt gccgcgtttg cgcaaaaatt cgcgcgggtc cagcgccgcc
    15061 atctcacgag tatcgggatc ctccagggat acataggcgt ggccgggaaa cgtgtccttg
    15121 accagggtcg ttttacccga ttgacggggc cccgtcacgg taaccaccgg gtattcccgg
    15181 gcaagcgcac ggagcgtttt cgctatctct cgcttgacca tttatgcaat tataaagttt
    15241 aattttaata ttacataaac cgccgatacc gcgtggcgtc aagaaaaaaa tgggatcaga
    15301 acatcgtgac gaagctgtcg agcgcggcga tgttcgcgca caccgcctga aggaccttct
    15361 ccgaggggaa caccgcggtg accgtgatgg agacgaagcg cccctcgcga ctctcgcgct
    15421 gggagatctc gggatcgacg ttgtgctcga ggaccaggct cctgatggtg tcgcgcgtaa
    15481 agggcgagct ccggaacacg gccttgaagg tcacctccgt ggggaagtcg gggggcaggc
    15541 gtcctgcggc gtcatccgcg atggtcattg catcaacact ccgttcattg ttcccgtcaa
    15601 cgattgcatg ccgcccaccg gcacggcgtt gggtgatgat gccgaccggt ttgatgcaac
    15661 ccttcgcata tcaatagaaa cgcgccatcc acgccgcatg cgcgatcgag catgcgcatt
    15721 cgtgacgatg ctgtcaagca catgtgccgc cggttttttt cttgccggga agcgcgtcct
    15781 ccattcagat agcgccatcg gaacaaattt tcgccgattc ccggcccccg gcgatgcgca
    15841 ccgcgtgggc cggattaccc tatacggcaa gagcggtcga tgccccatac aaacctgcag
    15901 gaattcatac gcgcactcga ggccgccggc gacctgcgcc gcatcgccgc cgcggtcgat
    15961 ccgcgcctcg agatcaccga gatcgccgac cgcgtgagca aggccggcgg accggcgctg
    16021 ttgttcgaga acgtgacggg atcgtcgttt ccgctgctca tcaacgcctt cggcagcttc
    16081 ccgcgcatgg agcgcgcgct caactgcgcg agcttcgacg agatcgcgcg gaaaatcgag
    16141 acgctggtga aggtgcagcc gccgcagggg atcatggaca aggtgcgcct gcttttcacg
    16201 ctcaaggaca tcgccgggat catgcccaag aaggtgaaga gcgccccgtg ccaggcgaac
    16261 gtgatccgcg acgagcgact gctcgaccac ctgcccattc tcacctgctg gcccggcgac
    16321 ggcggcccgt tcgtgacgct gcccatcgta atcacgaagg accccgacac cggcatccag
    16381 aacgcgggga tgtaccgcat gcagaagttc gacgcgcgct ccaccggcat gcactggcag
    16441 tacaacaagg acggcgcgcg ccactatgaa aaattcaaag cgctggggaa acgcatggag
    16501 gtcgccgtcg cgctcggcgg ctcgcccgcc gtgacctatg cggcctcggc gcccctcccc
    16561 ccggacgtgg acgagatgct cttcgcgggc ttcctcgagg gacgcgccgt ggagctggtg
    16621 aaagggaaga ccgtggacct gtacgtgccc gccgagtccg atttcgtgat cgagggcttc
    16681 gtggacccgg gcgacgagac gatcgaaggc cccttcggcg accacacggg cttctactcc
    16741 ccggccgaca cctatccggt atttcgcata acgtgcataa cccaccgaga cggcgcggtg
    16801 tacccggcga ccatcgtggg caagccgccg atggaggact gctacatggc caaggccacc
    16861 gagcggatct tcctgccgtt cataaagctc atcgtgccgg agatcagcga catcaacctc
    16921 ccgatggagg gcgtgttcca caattgcgcg ttcgtttcca tcaagaaaca ctaccccggg
    16981 caggccaaga aggtgatcag cgccctgtgg ggattggggc agatggcgtc cacgaaattc
    17041 atcgcggtct tcgacgacga catcgacctg gccgactaca gcacggtcgc ctggaaactg
    17101 ctcaacaacg tggacccgcg ccgcgatctg gtcttctcgg agggaccgct ggacgcgctg
    17161 gaccactcgg cgccgcacgc gaacttcggc gggaagatgg gcatcgacgc cacgcgcaag
    17221 acgcgcgagg agggcatggg ccgcgactgg ccgccggaga tccgcatggc cgacgacatc
    17281 cgcgacctcg tggacaaacg atggagggag tatggacttt aggaaattcg cgaatctcat
    17341 catgatcgag cagacgctct tcgcgctccc gttcgcctat atcggcatcg tcttcgcagg
    17401 cgggggatcg gtgtggacct ggatctgggc caccgtggcc gtcgtcgccg cgcgcacggc
    17461 gggcatggcg ttcaaccgct acttcgacgc ggagatcgac gcgaagaacc cgcgcaccaa
    17521 gaaccgcgcg cttcccgccg gcgaggtctc gaagaccgag gtgctctcga tcgcggtcgc
    17581 ctcgtccttc tttctcatcg tggcggccta tctgctgaac accctgtgct tctacctctc
    17641 ctttctcgcg gtggggatgc tcgcgaccta ctcgctcttc aagcggttca gctcgtcgtc
    17701 gcacctgtat ctgggactca tcgaggcggc ggcgcccgtc ggcggctacc tcgccgtcag
    17761 cggcgatttt cagctcatgg ccttcgtgct gggcggcgcc atcatggcct ggatcgcggg
    17821 gctggacatc gtctacgcca tccaggaccg ctccttcgac atggaccaga agctcttctc
    17881 cgttccggtg cgcttcggtg tggagcgcgc gaagctcatc tcagcgggcc tgtacgtcgt
    17941 ctcgctgggg gcgctcgtcg ccgcgggcct gctcgcgggc aagggcgtcg tctactggct
    18001 cacggtcatt tgcgtggggt tggtcttcat caaacaacaa ttgctcgcgc ggcagcagga
    18061 catcgaggag gcggtgcgcc acatcttcca gctcaacaac ttcgtctcgc cggtgctgtt
    18121 ggcgggtacg gtgctggatt tgtttattaa gctgtaagac ttaggtccac cccgcttccg
    18181 gcagcttgat cgtacgcttc gccgggtcct tccggggacg gtacatgatc gcgacattgc
    18241 cgatgatccc cacgagcgcg cccccggtcc gctccgcgca cgccgcggcg atctcatggc
    18301 gctcctcctt gcatcccacg aacttcacct taatgagctc gtgcgcctcc agcgcctcgt
    18361 cggcggccgc ggccagcgcg tcggtgaggc cgagcttccc aatctgcacc accgccttca
    18421 ggtgatgcgc cttcttcttg aggtatgcga tgtcggaatg cgatagtatt ttcatgattt
    18481 tcctgtgtac gtatccacgt aaaacatcgt actgcttcca cggcgtcaag cgtaccccgc
    18541 cttccctcga ctaagctcga aacaagccct tcgactgtgc tcaggacaag cccctcgact
    18601 atgcttgggg caaacccctc gacctggctt ggaaaacgct cggcggggac gggcgttgca
    18661 ccgagggtgt cgaagcggtt gaggcggcac tacttcccgt tcttcgcggg tatatactcg
    18721 atctcggcgg cggtgaccag cgacacgtta ttatcgagaa agccgctgat gcgcgcccgc
    18781 aggcgctcga tcgtctcgcg gtcggcgcgc tgtcgctcct gctcgctgcg aaagagggcg
    18841 aggtcgatct ccgcctcgat cgcctcgcgg atcatccgtt cgaggaggtc cggcgggagc
    18901 gcgtcgagct cgacgggaag ctcgccgaag cgcgcggcgc gtcgcctgcc gccgttcgcg
    18961 gcgcccctga tcgcgtcggg gttggcgggc aggcggtggc gcgtcacgtc gtccctgccg
    19021 agcgcgatgc gcttgaagga tatgttcgag agcgactcct ctttcgcgaa ggtcgccttc
    19081 atggactcca ggaggtccgt cccgccgggc gcgtaattgc cgaaaaagag gatcaccgga
    19141 cgtcgcccct cccggacggc gtcgaaggcg cggttcatga aatcgttcag gaagctcacc
    19201 gacgagaagc cgccgcacag cgccatcgac acgctgtagc gccgcgcgat cgcggagaaa
    19261 aaggtgcgca tcgagggcgc atccacccag agctccaggt aggcgtcctg cgtttgcatg
    19321 agattgcggt aatagccgga catgagcgcc ttgaggctct cctcgacgaa caccctcgtg
    19381 tcgtagcagc cggacagatc gtagaagccg ccggggcggt cgtcgatatc ggcccacgag
    19441 atgagcccct cgacgcgcgc gtgcttgcag atcgacgcga ccgcgccgaa ggcggcgcgg
    19501 ctgttctcca cgcgccgctg cgcgaccagg cggtagtaga tctggcgcag cgtgagcggc
    19561 aggaactcct cgaactcgtc gaggaccgcg tacacctgat cgagccgctc gcgcgactcg
    19621 tcgttccagc tccatttttt cggcggcatc gccttccccg gggtagaatg attgcgtcta
    19681 cggtgcgccg gttcggccga ttatgcaatc aaaaaaatga ttgagaataa tgggccccgg
    19741 cgggagggta ccccatgcac gtcgtcgcga taaccggcgc aagcggcgcc atcatcggca
    19801 ttcggctcgt cgaggagctc ctgcgctccg gcgcgcgcac ggccgtcgtc gtctccgaca
    19861 ccgcgcgcgc ggtcatccgc cacgaggtgc tccgcgacgc cgactacgcg accatgcgcg
    19921 cgctgctcga gcgccgcggc cgtgcggtcc ccggcgatgc gctgcgcgag ttcgatcccg
    19981 gcgatctctt cgcgcccatc gcgagcggat catttcactt cgcctccatc accgtcgcgc
    20041 cgtgctccat gaagaccctc tccgccgtcg cgcacggcca tgccgagggc ctcatcaacc
    20101 gcgccgtgga cgtcgcgctc aaggagcgcc ggcgctgcgt gctcgtcccc cgcgaaacgc
    20161 cggtgagcga gatccatctc gaaaacatgc tgcgcgcgcg ccgcgccggc gccgacatcc
    20221 tgcttcccgt tcccgcgttc taccatcatc cgcgctcgct cgacgacgtg gtcgatttca
    20281 tcgtgggccg cgcgctttcg cttgtcggta tcgagcacgc cctttttaca caatggggtg
    20341 atgatgcttc gcattgacgc catgctcgac ccccggttgc ggcccgtcgc ggagaaggcg
    20401 ctcgcgggcg agccggcatc gaccgaggac gcccgcgcca tgctcgaaac ggacgacatc
    20461 gtgggcctgg gcgcgatcgc caaccaccac cgccgccgcc tgcacggcga ccgcgcctac
    20521 tacggcgtga acatgaacct caactacacc aacgtctgcg agctgcgctg cccgctgtgc
    20581 gcctattcga aggacgccgg cgacgagggc gcctaccgct acacgctcga cgagatcgag
    20641 tcgcgcgtgc gcgacgccgc cgagcggggc atcgacgagg tgcacatcgt gggcggcctg
    20701 catcccgaac tgaagctcga ctacttcgag gagatgctgc ggcgcatcaa gcggatccgt
    20761 cgcgagctgt tcatcgtggc cttcaccgcg gtggaatacg attatttcgc gcgcataaac
    20821 ggcctcgcgc tcgaggaggt gttccgccgg ctcatcgacg cggggctcgg ggcgcttccc
    20881 ggcggcggcg cggagatctt cgcgccggag acgcggcgcg tgatcgcccc gaaaaagatg
    20941 agcggcgagc gctggctcga ggtgatggag accgcgcacc gcatggggct caagaccaac
    21001 gcgaccatgc tctacaacca catcgaaacc accgaccaga tcgtggacca tctgacgcga
    21061 atccgcgcgc tccaggagaa gacggccggc ttcaagacct tcgtgccgct tcccttccac
    21121 gcggagaaca cggccgttcg cacggcgcga cccccgcgca ccggcttcgg cgacgttcgc
    21181 atctacgcga cggcgcgcgt attcctgcac accgtgccgc acctcaaggc cctgtggatg
    21241 tacctgggcg agcgcctggc gcaaacgatg ctcgccttcg gcgcggacga catcggcgcg
    21301 acgtaccatc acgagcgcgt ggtgcacgcc gccggtgcga ccagcgccgc gagcggctcc
    21361 gaggcgcgcc tgcggcggct catcgagcgc gcgggcttcg tcccggtgcg cgcgaacgcc
    21421 gcctacgaaa ccatcgatcc ggaggcgcgc gcatgaggct gggctgcatc gactatctca
    21481 actgctttcc cttctactat cacatgctcg agaagcagcc gcttcccggc gtggagatcg
    21541 tctccgccta cccgtccgcg ctcaatggca tgatgaacga gggggcgctc gacatgggcc
    21601 ccatgtcggc ggcggagtac gcggacatcc gcgagcgcac gctcctcctc ccgcagttcg
    21661 cgctgagctc cgtcgggtat gtgaaatcgg tggtgctgct ttccaatcac cccatcgagg
    21721 agctttccgg aaaaaccgtg ggcctctcga acgcctccaa gacctcggtg gtgctcctga
    21781 agatactgct gcagaaacac tacggcctgg cgccccgcta cctccccgcg ccgccgcgcc
    21841 ccgacctgga ggcgctcgac gcggcgctgg tgatcggcaa cgaggcgatg atggacgacc
    21901 ggcagcccgt tcccttcagc tacgacctcg gcgacctctg gctcaggcga acgggccatc
    21961 cggtcgtctt cgcggtcttc gccgtccagc gcgagtacgc gcgggcgcac gaccacgagg
    22021 tccgcgcggt catcgactcc taccgcgcat cgcttgaccg cctcgcctcc gagcgcgagc
    22081 gcctcatcgt cgccgccgcg cggcgctacc ccgacatccg ctacgacatc gaccactact
    22141 acacgctgct gcagttcgag ttcaccgatg cgctcaagaa cgccctccag ttctatttcg
    22201 acgaggcggc ggcgctctcg ctccttccca gggtcccctc gctcgtgttt tttccgcact
    22261 aaccaacccc gactggcgca cggcgcatca tcataaaaat tttttcattt tttttgatga
    22321 ttggattgac aaccgccact gtttccagtt catggtaaca gatcgtgata tcagatgttg
    22381 atatcaacat ttataaccaa atgggttgaa acgatgaccg aaacgataaa agcgcgaaag
    22441 gcctcaatgg acggcaacac cgcggcggcg catgtcgcct acgccttcac cgaggtggcg
    22501 gcgatttacc ccatcacccc atcatcgacc atggctgagt gtgtcgatga atgggccgcc
    22561 tatggcaggc ggaacatctt cggcgagacc gtccgcgtgg tggcgatgca gtccgaggcg
    22621 ggcgcagccg gcgcggtgca cggatccctg caggccggtg cgctcacgac caccttcacg
    22681 gcctcccagg gcctgctcct gatgatcccc aacatgtaca agatttccgg cgaactcctg
    22741 ccgtgcgtga tccacgtgag cgcgcggtcg ctctcgaccc acgcgctgtc catcttcggc
    22801 gaccattcgg acgtatacgc cgctcggcag acgggattcg cgatgctcgc aacggggagc
    22861 gtgcaacagg tgatggactt ggccggcgtc gcgcacctgg ccgccatcgc gtcccgggtc
    22921 ccattcctgc actttttcga cggcttccga acctcgcacg agatacagcg catcgaggtg
    22981 ctcgactacg acaccctcga cgcgctggtg gaccgcgagg cgcttgcggc cttccggaac
    23041 cgggccctct ctcccgagac gccggtcacg cgcggcaccg cgatgaaccc ggacatctac
    23101 ttccagctcc gcgaggccgc gaacacctac tacgatgcgt tgcccgatat cgtcgagcgg
    23161 tacatggccg agatcggccg cattaccgga cgcgagtacg cgccgttcac ctactatggc
    23221 gaccccgacg ccgagcgcgt gatcgtcgcc atgggctcgg tgaccgagac ggtccgcgaa
    23281 acgatcgatt acctgcgcgc gcacggcgag aaggcgggtc ttgtgaccgt ccacctctac
    23341 cgtccgttct cggcgcggca tttcttcgcg cgtctcccgc acaccgtcac gcggatcgcc
    23401 gtgctcgatc gcaccaagga accgggcgcc ccgggcgagc cgctgttcat ggacgtcgcg
    23461 acggcgttca tcgggcgggc gtcggcgccc gtcatcgtgg gcgggcgcta cggcctgagc
    23521 tcaaaggacg tcacgccgca gcatatcatg gcggtgttcg aacatcttac ggaagcctcc
    23581 cctcggcacg gattcacggt gggaatcacg gacgacgtga ccaacacctc gctccgcctg
    23641 ccaccggagc ccgacatatt ccccgccgag actttcaagg ctcgctttta cggcgtgggc
    23701 tcggacggca cggtgggcgc gaacaagaac tccatcggca tcatcggaga cagcgcggac
    23761 ctcaacgccc aggcgtattt cgcctacgac tccaagaagt ccgggggggt caccgtatcg
    23821 cacctgcgct tcggggaaaa acccatcacc gcgacctacc tggtgacgcg gccgaacttc
    23881 gtggcctgcc acgtccccgc ctacctcggc cagtacgaca tgctgcgcgg cctcaagccg
    23941 ggcggcacgt tcctgctcaa cgcgccctgg gacgacgagg agacgaagcg gcacctcccc
    24001 gcgtcggtca agcgttatct tgcgcataac ggcattcgct tctacgcgat cgacgcctcg
    24061 cgcatcgccg aggaggtcgg gctgccgggc cgcaccaaca gcatcatgca ggcggccttc
    24121 ttccgcgtgg ccggcgtcat tccccacgat gaggccgcac accgcatgaa ggaggccgtg
    24181 aagaaatcgt acggcaagaa gggcgacgat atcgtcgaga tcaactgcgc cgccatcgac
    24241 cgcggcgccg agatggtgcg cgagatcgcg atcccgcccg aatggaagga cgccccggac
    24301 gatgcggccg acgccgcctg cggtaactcc ttcgtcgata ccatcgcgcg ccccatcaac
    24361 cgcctcgaag gaaacgatct tccggtgagc gcctttacga gcatgccaga cggcaccctt
    24421 ccgcagggaa cggccaccta cgagaagcgc ggcatcgcca cgcacgtccc cgaatggatt
    24481 ccgtccacct gtatccagtg caaccagtgt tcgtacgtgt gcccgcattc ggtgatccgc
    24541 cccttcctgt tggacgacgg cgatgtcgcc gcgcttccgt tccccctcga cctccttccc
    24601 gcgaagggga agggcctcga ggggcatcgc taccgtatcc agatcagccc gctcgactgc
    24661 tatggctgcg ggaactgcgc cgaggtgtgc ccggtcaagc agaaggcgct cgtaatgcgg
    24721 cccttcgaat cgcagttggc ccagcgtccc atatgggacc acctcgccgc gaaggtgtcc
    24781 tacaaggatc atctgatggc aaagacgacc gtaaagggga gccagttcgc caaaccgctc
    24841 ctggagttcc acggcgcatg cgcaggctgc ggcgagacgc cgcactacaa gctcatcacc
    24901 cagctcttcg gcgaccgcat gatgatcgcc aacgcgacgg gttgctcctc catctattgc
    24961 ggctcggccc cgtccacgcc gttcacgacg gacgcgcgcg gccgcgggcc cgcctgggcg
    25021 agctccctgt tcgaggacaa cgccgagtac ggctacggca tgcacctcgg atattcgaaa
    25081 ctgcgcgcgc gagcggcccg tctcatcgag accctcgccg cgggcgcgcg cgacggagcg
    25141 ctgcgtgacg cgtcggccca atggctgcgc gtaatggacg atgcgagcgc atgcgacgcc
    25201 gcgacggacc ggctcgtcga acgtgtgcgc ggttcgacgg acgaaacgag cgccgaggtc
    25261 gatcggttgc gcgcctattt gcgcaagcgc tcgatctgga tggtgggcgg cgacggctgg
    25321 gcctacgata tcggctttgg cggcctggac cacgtgatcg cgatgggcga ggacgtgaac
    25381 atgctggtac tggacaccga ggtttactca aacaccggcg gccagtcctc caaggcgacg
    25441 ccaacggccg ccgtggcgaa gttcgcggcg tcgggcaagc ccctcggcaa gaaggacctt
    25501 ggactcatgg ccatcaccta cggctacgca tacgtggcgc aggtgtcgat gggcgcgaac
    25561 caggcgcagt ttttgcgcgc cgtgctcgag gccgaggcgt atccggggcc gtcgctcatc
    25621 atcgcctatt cgccgtgcat caaccacggc attcgctcag gcatggaaac cacacagcgg
    25681 gtcatgaaga acgccgtggc ctgcggctac tggcacctgt accgctacaa tccgctcctg
    25741 gagcgcgaag ggaaaaaccc cttcgtgctc gattcgccgg aacccgactg gggcgcgttc
    25801 cgggaattcc tcatgaacga gacgcgctac acctcgctcc aacacgagtt cccggaacac
    25861 gccgacgggc tcttcgcggc ggcggaagac gacgcgcgga gacgctacaa cacctatcga
    25921 cgccttgcgg acagcatggc ggcatcaacc gacgtaccgg aggcatccaa tgagcgcgcg
    25981 taagttttcc atcgtcgttc cggcactctt catcctcggg ggcgtttttc tggttccctt
    26041 cggaacgaaa ccctcgaccg gtcagaccgc gaaaaaggca ccgacaaaac agacgtatgc
    26101 ctacccgtcg ttctacaagg gcttctatct cacctccggg tcgggaaaca atcgggcaaa
    26161 gctcgagcgc cacatcacgc gcgcgaaaaa ggcgggtctc aacgccgttg tcatcgacgt
    26221 ccagacgccc gggatgcgcg tcgccgtcac gccggccgag cacgtggcct actgccgcgc
    26281 gatgggcttt cacccgatcg cccgcgtggt ggtgttcccg gagggcctct ccgagtaccc
    26341 ggccgccgcg gagctcatcg agaaccgcct ggccgtcgcg gaggccgcct gccgcgcggg
    26401 ctatcgcgag atccagttcg actacatccg cttcaacgac caccgcgtga accgggcgct
    26461 gtcgctcgcc gagcgctacg agtacatcac gggcttcctc aagaaggcgc gcgagcgcct
    26521 ctcgaaatac gacgtgcgca tcgccgccga catcttcgga cggataccgc tcaacgccga
    26581 cgataaaatc ggacagaaaa tggagctcct cgacggactc gtggacgtca tctgcccaat
    26641 ggcttacccg tcccactaca cctggagtaa aaagatgatg gccgatccgt actataccgt
    26701 ccacatcacc tcgaagcgcg cgctggagcg cgcaccgcgc tccgcgatcg tcacgtggat
    26761 ccaggccttc cggatgaagg tgaagcgctc gaaactcggc tacgaccggt acgtcgcggc
    26821 gcagatcaag gcggtgcacg attccggcgc cggcggctac ctgctctgga acgccgcgca
    26881 ggactacgcg atcccgctcg cggtggcggg cgagataaac cggcgccgcg cacgcgtggc
    26941 atcgttatcc gaaacaccat cgggaggaaa ctgacatggg aagcgtaaaa gacctcaccg
    27001 tcatcaacgc gccgtccgag acgcaggccg gcctgggccg gttcgcgttt tccgaccgct
    27061 actcggtgtt cgactggggg gagatgcccg accacatcga tggcaaggga gcggcgttgt
    27121 gcgtcatcgg cgcgcacttc ttcgagctcc tcgcccggga gggggtcccc gtccactacc
    27181 gcgggctctt cgacggcgac ggccggatca agcggctggg cgagctcacg gctccgtcct
    27241 gcgacatggc cgtgagcctg taccgcgtcc tgcggccggc cgagcgcgac ggaagctacg
    27301 actacgcgcg gtacgcggac gtgaacaaca actatctcgt cccgctcgag gtcatctacc
    27361 ggaaccgcct tccggagggg gcgagcgtct tcgcccgact gcgggaggga acggtcacgc
    27421 tcgccgagct gggcctcacc gagatgcccg cgcccggcgc catcctcgac cggcccatcc
    27481 tggacgtctc gaccaagctg gaggtgaccg accgctacat cacgtggccc gaggcgcagg
    27541 ccatctccgg cctcctcgac ggggagatgg acacgctgcg cgggctcacg ctgcgcatca
    27601 acgagaccat cacgcgcgag gcggagcgca tcggccttgc gaacgaggac ggcaagatcg
    27661 agttcgcgat cgacgagcac gcgcgcctca cggtggtgga cgtgctcggc acgcccgacg
    27721 agtgccgctt cacctggaac ggactgcacg tgagcaagga gctcgcgcgc gtcttctacc
    27781 gcgggagcgc ctggcacgcg gcggtggagc gcgcgaaacg cgaggaccgc atcaactgga
    27841 aatcgcgcgt cgatggcgcg ccggcgccgc ttccggcccc gctcgcgcgc aatctttcgc
    27901 acatgtacca ggcggtggcc aacgggctca ccggcaggga cttctttccc gtcccgcccc
    27961 tggccgaggt catgaaacag gtcgcgctcg aactgggcgc cctgcgacgg gagggctgat
    28021 atggctgatg tgatgattca cgagttcctg ctgggattca taaagatcta catcctgcag
    28081 ttcgccgaca aggagcccgt ctacggaaag gagatccacg accagctcga ggagttcggc
    28141 ttcaagatat ccttcgggac gctgtacaac accttccacg gcttcgagga gaagggctac
    28201 cttgtccacg aagagcggaa cgtgaacggg aagatccgca agtactatcg cataacgaaa
    28261 cacggccgcg aggcgctcgc gacggcgaag ggcaaggcgc gcgagctctt tacggcgctg
    28321 tacgaataga atacacatac cacaacggag aacggtacac catgaaactg tcacaacgga
    28381 tcaaaaacgt aaaaccctcg gccacgctcg cgctcacggc gctggccaac aagctcaagg
    28441 cgcagggggt cgatgtgatc ggctttggcg ccggggagcc ggatttcgac acgccggaac
    28501 cgatcaagga ggcggcgcgc cgcgcgatca acaccgggaa aacgaagtat acgcccgtag
    28561 gcggcattcc cgagctcaag gccgccgtgt gcgagaaatt caagcgcgac aatgcgctct
    28621 cgtacaaacc cgcggaggtg acggtcaact gcggcgggaa gcactcgttc tacaatctca
    28681 tgcagtcgat cctcgacgag ggggacgagg tgatcgtgcc cgcgccctac tgggtatcgt
    28741 atccgccgat catcgcgctc gccggcgggc ggccggtgat cgtcgactgt ccgggcgaac
    28801 gcggcttcaa gatgacgccg gaacagcttt cggagcacgt gaccgacaac acccgcgcga
    28861 tcatcatcaa ctcgccgtcg aacccgaccg gaagcgccta cacgcgcgcg gagctcgcgg
    28921 cgctggccgg ggcgatcgcc gggcgcgaca tcatcatcgt gtccgacgac atctacgaat
    28981 cgatgctcta cgacgcccgc acgttctgca ccatggcgaa catctccgac gagctgaagg
    29041 cgcgcaccat cgtccttaac ggcgtatcca aggcctattc gatgaccggc tggcggatcg
    29101 gctacatggc cggcgacgcg gcgatcatcc aggccgtcga gacgctgcag agccagtcca
    29161 cctccaaccc gacctccatc tcgcagtggg ccgccgtcga ggcgctaacg ggcgaccagt
    29221 cgttcatcgg cgagatgatg accgccttca cgcggcgtcg ctccctgatc gtggacggcc
    29281 tcaaccgggc gccgggcatc tcctgccagc cgccggacgg cgccttttac gccttcccgc
    29341 gcgtgtcggg cgtgttcgcg ctccccggct ggccggcggt gcgcgagcga tatgcgggac
    29401 aggcgaactc ttccgcccta tgctcgcatc ttctcgacga ggcgcgcgtg gccgtggtgc
    29461 cgggcgtgga gttcggcaac gacgagtacc ttcgattgtc cttcgccact tcggacgaga
    29521 acatcacgcg cggcgtggag cggatctgcg aggccatgca gcgcctggcc ggctaagggg
    29581 tgccgcacgg acaatcggat tgcttcgtcg ccaccgcttt tcgcacgaga ccgaggtacg
    29641 cgatgaatgc aatagcgcag cgaacacgag aatacaatca ggagacagta tatggtggga
    29701 tgcagttatg gacgtttctt tcaggtgacc gtcgcgggcg gctcgtatca ggagggcctc
    29761 gtctcgcaca tgcagggggt gccctcgggc atccatctcg aggagaagga catctacgcg
    29821 gatctccttc agcgcaagcc cggccagggc gagctctcgt cgccccgccg cgagcccgac
    29881 atcccggtga tcgtcaccgg cgtgaacgcg gccgacacca tgccgggctt cgccaacgcg
    29941 gagcacacca acgggacgcc gctcaccgtg ctcatcccca acctggaccg gcacttcgag
    30001 cacatcgacc agtatcagcg caccaaccgc acgccgcgcc cgggccacgc ctcctacgca
    30061 acgtaccaga agtacgggtc cttcgacgac tccatcggcg cggggatctt cagcgggcgc
    30121 tacacctcca ccatcgtggc cgccgggtac gtggccaagc gcatcctgcg cgaccacggc
    30181 atcgaggtct tctcgtacgt gaaggaggcg gccggggtgg cgatgcccga aatgccgtac
    30241 gacgccatca aggagcgggt gcgcatattc cgcgagttcc gccgggacaa tgacccgttg
    30301 tacgccatcg tgtacgcgca gggccgcatt aaacccggca tgcgcatgct cgagaagatg
    30361 gcggtgctgg cggagatcga gcgcatggtg cccgacatca cgcgcgagcc gctctccgat
    30421 gcgcaggttg cggccctggc cgagcagggc gtgcaccccg tgctcaactg cccgcacctc
    30481 gaggcggcca cggagatgta taataagatc ctggagatac gcgacggcgg cgattccagc
    30541 ggcggcctcg tggaggtggt cgcgacgggc gtcccggccg gaatgggcga gccggtcttc
    30601 gaaaagctcg acggcgagct cgggcgcatg atgtccatcg gcaccatcaa ggcggtggag
    30661 atcggcgcgg ggatgcgcgt gaaggatatg accggcagcg agtgcaacga ccagatgcgc
    30721 gcccgcgacg gccgcgtcga gttcatgagc aacagcgccg gcgggatcac cggcgggatc
    30781 agcaccgggc aggacatcgt ggcgcgcctc gcggtgaagc ccacgcccac catcgccaag
    30841 ccgcagaaga ccatcgacaa ggtctcgaac accgacgccg agctcgccgc ggtcacgcgc
    30901 cgcgacccca ccatcgtctc gcgcgtgtgg ccggtggcgg aggccttcac cgccatcgtc
    30961 ctgctggacc agctgacgct gcatatcgcc tatcaggcgc tacggtcaca ctacaaggaa
    31021 cggtaatcga acaatggaac gagcgatact cgaatacaag ggcgcgcgat acgaactccc
    31081 ggtgatcgac ggcacggagc aggagcgcgc catcgacata tcgaacctcc ggaaggactc
    31141 ggggctgatc acgctcgacg tgggatacca gaacaccgga tcgtgcacct ccgcgatcac
    31201 gttcatcgac ggcgaccgcg ggatcctgcg ctaccggggc atccccatcg aggtgctggc
    31261 ggagcgatca accttcacgg aaactgcgtt cctgctcatc tacggccacc tgccggtcga
    31321 ggcggagcgg cgcgccttct ccgagcgcct cacggagaac tcgatgatcc acgaggacat
    31381 gctccgcttc ttcgacggct tcccgccgac ggcgcacccg atggcgatcc tctccaccat
    31441 ggtgaacgcg ctgtcggtgt actaccccgg ctacttcacc gaggacttcg agagcggcac
    31501 cttcgacctc atggcggtgc gcctcatctc caagatcagg accatcgcgg cctactcgtt
    31561 caagcactcc atcggcgagc cgttcgtgta cccccggcac gacctccggt actgcgcgaa
    31621 cttcctgtcc atgatgttcc acagcccggt gaagccctac gagatcgacc ccgacatcga
    31681 gaaggcgctc aacgccctgc tgatcctcca cgccgaccac gagcagaact gcagcacctc
    31741 gacggtgcgg ctggtgggca gcagcatggt gaacctgtac gcctccatct gcgcggggat
    31801 ctgcgcgctc tgggggccgc tgcacggcgg ggcgaaccag cacgtgatcg agatgctgga
    31861 ggggatccag cagcaggg
//
LOCUS       DAORHF010000011        31275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_839_length_31275_cov_5.023062, whole genome shotgun sequence.
ACCESSION   DAORHF010000011 DAORHF010000000
VERSION     DAORHF010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 31275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31275
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_839_length_31275_cov_5.023062"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..2443
                     /locus_tag="PKJ16_01980"
     CDS             200..2443
                     /locus_tag="PKJ16_01980"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HNV45780.1"
                     /translation="MRTKYVTFVLLTVFSALALSGCYEQGSLYSKVSSRTLPAGDFLV
                     HWSNPGEGKAFVAVRHRSNPGRVLWSTEPGQNFLRAIMADIDVKEHRGSFTVKDDERL
                     SCAFQSVDDVRLDGAAVTITGRLADAKAKNGASYVMTFRQASGGHLRFEASVAKIGCA
                     GEFCARAFNQIEISYASSSNEKFYGFGEQFTHLNLKGHRVPIISQEGGVGRGREPVTS
                     ALNLVSPGSGGNAYTSYISVPHYITSLMRSLFLETTEYAVFDLRDDESVSVKIFSDRI
                     AGRILYGANPLELIERYTEYAGRMKALPDWIGSGAIVGMQGGTERVYEIWNLLEAQGT
                     PIAAFWLQDWVGKRKTMIGSQLWWNWELDALTYPEWDGLVAAMHEADIRAMGYINSFL
                     VDPVQKGSFARNLYREAVDGGFLVMNGNNQPYPIKNTDFDAGMVDLSNPDARAWIKSV
                     IKEEMIARGFSGWMLDFAEALPFDAVIASGDAASYHNQYPVEWAKINQEAIAELGLDD
                     EIVFFNRSGYTKSPGASTLFWEGDQMVTWDGDDGLKSAIKGLVSGGFSGFSLNHSDIG
                     GYTSVSLSVLNIFKREKELLMRWMEANAFTAAFRTHEGNEPERNAQFYSDADTLAQFT
                     RFAKVFRALGFYRAELMRDAEAKGWPLVRHMLLHYPNDPAAYDLKYQFMLGADFIVAP
                     VVDKRADSVRCYLPAGEWVHVWTGASYNAGWVDVGAPMGKPAVFFKKGSPAGSRFVRN
                     LRGEGVL"
     gene            complement(2669..8908)
                     /locus_tag="PKJ16_01985"
     CDS             complement(2669..8908)
                     /locus_tag="PKJ16_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45781.1"
                     /translation="MKSIALLFTAAILSGFFAFGCSKTDGDQTFQQTEPNDTVMLFEV
                     LNESPALKSAFSNLEPREFNRKMNDLMFANPDLLGELMVRTGEALYLPETPGAKAIFP
                     ETVDKLSKSFNSFHDAYMRDPDSLDTTVDIVKAILDLDPTVMSEGAGVIMDLLVALRN
                     WDDVNSDGGYWVDANGNALFDSAEAYDPAETSAYPWRLFGQGQELESIGYAGVNTLIE
                     NISFARTLYGDGEGLQDLSTIIRKSIESVIEEHWDIEQKMFSFIDYLETNDPFTGITA
                     IEKEIADWMAPGADPLKYSIGEYLINDLFPKVQRPVFDIVTDGAVERSIDVLESGKKF
                     LNDESLYLSESAKRRSRKYNIGPDAAAQSNDDGLYSKWFLGALYHDMDWYDRLEDVPL
                     FDFVNNDILRWLKGDAARRGVVPRWNTVLANFPQDRMTDLLWNGHTYSPTAGASTFFP
                     GLVSPGGAVTALGRVLSSDALLKPFRRQVNDILNSDGLGGYDDNANGTFKTVGLSYSS
                     TGNDRRPENIIESVMTNLKFYTLSSYYKASEGKWAFGPEDGQAFFGDPNRNLQTLFGT
                     LTTNIRNLVILDRDGKDPRVPGYTGNGLSLLTNFVYLFAAGGGMVDPNNAKTELSLRN
                     VLNSLGSPLRSRNYLNAMDYVDDSIDWLLELFGSWRVYCLEDATIRRSPSYGYHIASG
                     SIRPNTAPPSWTTFSSQYQMPVMEVLQPGTFRQRVGSNNPGAALHGVFSPSQGDLFGI
                     TRHNPATGNDVLNTSDWLNSEIALACWEGYGPYTYLGKAPNGSECKYRNDWYTDWYQM
                     IGMEWGNKGPGLKYDRGRYHYFEAIYKPTHNSRYGYFRKSSPVGMNITQDGSYANAVN
                     PDDPGTYDRQVVLDCATREEAMRKNFQWLLNYKKYVYLLPIHGMLDKDIITIPLINKS
                     LCNIKTEIYTYVTINFNGIGGAARGRRHQAAPNDKTNNAVWSGGIEHVYDNTMAIDSN
                     HVVNDDAGNPTYPVKLIEESNATFRMAASSFRPQDFCVAMEYDYSSKITLLFDLIPLP
                     DNMSAGILSMLLSIVDALWSAVGNGSILPAVIGDNLQALITLGDSVYSARDVLGAASP
                     TSDAMTKFNEFYRTYYTMSYVGPGATAGTLIYRDDNGREQSIKEKDLPRIPRVAGVSY
                     PTSFTDSGQATAWRVYEGETEGKFDLFVALYAAIQGTLHEDGRIMGFDGVGNVVPADY
                     SAIDRGVIAYYDRDGYRTQINDFVMTVCALNEARNAPGGTAPIPDPSSLLNLLVDRGG
                     MAGDAIGARNGVLSYVIESTYANLNNLNPIKESVEGAVRNVVRNFLNNGGNFRLTATG
                     ADAPLPYYVTREDGTRAVSNRHNWETPINKLRYFTDNESLRRLEESLDLLRDVSRSDE
                     FKEMLRKTIIALDNFLIVKHIDEQYRTCVKACDCSGIEDEPARAACRANYDACTAGCV
                     KMTRVEALNDGVLQLYTPLDDAGNQLAPGAANYDAEKAAQLAADIQKAADVLIETDYH
                     QIIDFIKRSGINDIEKLYNFSFDNWSLDATGLTMSDVRKNIDDINATLVKLAGVNLKE
                     GLIDGIYRLRNDAGTVVPDPRYFKRGDTIYGFGTYVEITQAEYQNGEAPADVTYVDFT
                     DGEGALLRYYRYIDIRRIMMPSGVLDDEHYSTDFGGITAYFDFRNDGTLGNAYSELCD
                     NYRDPWYRAGTVDCPNVFNPHKYDINMEGIFGGDATRFAAGDFATVPRASRVLTDFTA
                     GITDENGMPLAFDRPHRYYRPTTFIDTLYRWGDGLDIETELDYAKDSIIHSVYEEREM
                     EVPELLRGYFTSTDYRDETNHRASIRTVIQKSRGVMIEKAYDDGYDYDMNPGNGAVTH
                     YVAQEPAGARHRHKLNNIADIIAILLNPGDPDHPDEEYRTNPHFLTGRINTAWNAFIN
                     SPSIQDITTDDLVKVRSAVASLLYNSDESCQTCDDRGENCRPCTRDDRYSRLFTNLTA
                     NLPKLLRKYQLTVERDGDVYMQYGDTLYDELAQLGLVAFSPDGVGTYLFENIRPAAQF
                     TAWNMTEEIDSLLRKDIFTTSSTGTFWFEIGHLIQSFGIILKQREIVNGEMLDYYSSF
                     RGMFE"
     gene            9492..11210
                     /locus_tag="PKJ16_01990"
     CDS             9492..11210
                     /locus_tag="PKJ16_01990"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV45782.1"
                     /translation="MPDLARARPGRARRTVVICALSALIALAAGAGPLRAPPNIATLA
                     ERNSLADEWRITASDRPEYSSRAYDDSGWDTIRMPGSFFGYALRKTGALYGTVWLRKT
                     IIIDARAERLPAVGLILGRIANADETYFNGERIGGMGRFLPEDNSMWNHPRHYLVPGK
                     LVNYGGKNVIAIRVSHYIFGEMIGTLALTDLDTWEQDRVWQNFTRVTAGYIIIAMGCL
                     FLVIFLLFYALRREQEYLYYALQMACGFFIVLELCNFWNTFGSNLMRLKALGLSWAAV
                     NVVHPIFLHRIYDLQRKKIEWALWVFLALTIGLLLTITGKPSDLLQVNIFITFTICIG
                     LYNISCHVSAIFKRRPYSRVFGFFGIAVALGAIHDGLVYLAKFTDYSISLFGYVFRDY
                     MIFHYMAIMLYTGTALVLVFRFISMATEVEDLKTTLENFIIENAILNKQVVRSRDEGK
                     KRPEESALTDRTMEKLQKVVAFINENYQSSLSREGLAASVDVHPDNLGKLFRRYTSKK
                     LGDYICELRVEDAARKLVETDEPVIDIAFATGFESLRTFNRAFLKFKKMTPASYKKKN
                     KPATTS"
     gene            11574..13631
                     /locus_tag="PKJ16_01995"
     CDS             11574..13631
                     /locus_tag="PKJ16_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV45783.1"
                     /translation="MFVHAGPSFAIEPVVLDETREEYILGKHIEYLKDAKKEYAIQDV
                     SAAGFDSRFTASTEDWPNFAYTSAAYWLRFTIRNASKETRRWILLDNYPLTDDIKLFM
                     PAQHGGKILKHAGRFLPYAARDEDYRAFAFHLDVPAGSEQTYYLRLESEDSLVAILSI
                     VSPKRFTQIKHNTQIMLGIYYGFILAMIIYYFFIFIATRDRNYIYYVLTLIFLHLLFQ
                     FSLNGLSAEYINQTSLWWSRGSIVFFEAIGVFFAMQVAKEFLQTKRNAPVFHKLYNIV
                     MYIVFAIALGSLFLPYYYMIISAVLMTMSGSVLMWSAGLYCYYKGFRAARFYLLAWTS
                     IQLLGCFYGLKTLGLAPSFFLSEWGFQIGSLTHALLMSFALADLINIMRIEKTTALKE
                     SISLKEEMNRNLEIKVKERTEELQQAMATMKAMNEEIVTARDALWGEMQLAKKIQTVL
                     LPVKPQMKGYDISVFLKPADDVGGDYYDIINVENIDWIVIGDVSGHGVPAGLIMMMVQ
                     TAIHTVLASKGNLKPEALLTRINRVITRNIRLLSEDKYMTLTVFACIKDGKFYYSGLH
                     QDIMIYRSTEKRVGLIGTNGIWIGLLEDIRGMVTGEHFSMAVGDVMMLFTDGITEAWV
                     EGSVRDNRTMETDMFGQHKLKEVFEEYGEHHPDDIKSGILSKLDGYRITDDVTMVIVK
                     RRE"
     gene            13904..14209
                     /locus_tag="PKJ16_02000"
     CDS             13904..14209
                     /locus_tag="PKJ16_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45784.1"
                     /translation="MKKLVEILSVDSVHIIALDARGNRVTIAIADCINNFYREFMGAE
                     YVVTGNDRMIGERNSQADPRYFIFHSNPRYCIEVDRDRFDAISDMLSGNNLQTFDQT"
     gene            14231..14611
                     /locus_tag="PKJ16_02005"
     CDS             14231..14611
                     /locus_tag="PKJ16_02005"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HNV45785.1"
                     /translation="MDNTLVDEFGASARPGVIDLLVHLKRTGHTLILWTNSRKERARQ
                     IILDNNLRRYFTTFIFREDYDPEEKGLSKDIRKVKGDILVDDDPAEIRHVATLGKHGY
                     LVASYRKGMTVDRNEFAGLLKMLR"
     gene            14622..14804
                     /locus_tag="PKJ16_02010"
     CDS             14622..14804
                     /locus_tag="PKJ16_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45786.1"
                     /translation="MRSTRHPVVGPPILRIIFAIFVEAAIFRYLPGVSVRRALKTSTI
                     ANAASLVFGLIFIAMI"
     gene            14839..15465
                     /locus_tag="PKJ16_02015"
     CDS             14839..15465
                     /locus_tag="PKJ16_02015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV45787.1"
                     /translation="MPSLMHRRSHHSMKRFYDWVHRYYGSIEESIGPALDSIIEGMPS
                     VFADAGSKTALDYACGSGLLTLKLARRFSVVDGRDASTGMIGRAMERANALSVFNASF
                     RAGDIMEPDEAPGSYDIVFVSFALHLFHPDVEREILRKLFAIATEAVVVIDHGRRWSA
                     KTALIEWIEGSYYDRFITLDFNAMANEIGASRFVETEIADSLVLEFCK"
     gene            complement(15558..16559)
                     /locus_tag="PKJ16_02020"
     CDS             complement(15558..16559)
                     /locus_tag="PKJ16_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HNV45788.1"
                     /translation="MALFESYERRIKQINAVLGAHGITSIEETKSICDAKGIDVAAIV
                     RGIQPICFENACWAYTVGAAIAIKKGVTAAPEAAEILGQGLQSFCIPGSVADDRKVGI
                     GHGNLAAMLLREETKCFAFLAGHESFAAAEGAIGIAKSANKVRKNPLRVILNGLGKDA
                     AFIISRINGFTYVQTKFDYYTGTLAIVKETAYSSGEKAKVRCFGADDVREGVAILHHE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECVDLGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGATVAVAVAVAIQQAR"
     gene            complement(16576..17268)
                     /locus_tag="PKJ16_02025"
     CDS             complement(16576..17268)
                     /locus_tag="PKJ16_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003442486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45789.1"
                     /translation="MIYSAEVEEMMCVAKGPDHGPAPIPEEGKWVQVKEIKDISGLTH
                     GVGWCAPQQGACKLTLNVKDGIIHEALVETLGCSGMTHSAAMAGEILPGKTILEAMNT
                     DLVCDAINTAMRELFLQIAYGRTQTAFSEGGLPIGAGLEDLGKGLRSQIGTIFSTKAK
                     GVRYLELAEGYVNQLALDTDDQIIGYEIVHLGKMMDMIKKGTDANDALKKATSTYGRF
                     DDAARKIDPRHA"
     gene            17387..17845
                     /locus_tag="PKJ16_02030"
     CDS             17387..17845
                     /locus_tag="PKJ16_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNV45790.1"
                     /translation="MKREYIDTALSHFDDGCACSQSILLAFSEVFNLDETVAKRIAST
                     FGGGMGRLREKCGAVTGAFMVLGLAFGNVDPKDMKTKLYAYEKVRELNARVQGEFGTT
                     LCAEFLRKKASAADIAARAHHRIVCRSIIEYTVGALVDILIENGRLSRDS"
     regulatory      18002..18062
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            18139..19383
                     /locus_tag="PKJ16_02035"
     CDS             18139..19383
                     /locus_tag="PKJ16_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006341662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV45791.1"
                     /translation="MLELFTIVTAWLALAVFSTILANRLKISMALMEICVGAFAGYIA
                     MRFFDPDILHPNAEWMKFVAGSGAVLLTFLAGAELDPASMRAKAKEVTVVGLIGFFAP
                     FIGCAALAYFLLGWDGRASLLGGIALSTTSMAVVYAVMLEYGFNKTEYGKGILGACFI
                     NDLGTVIGLGLIFAPFTYRTLILVAACVLIVFVLPPITDRLTKVYGNRTAAIRTKWLV
                     FVLFGLGALALWSGSEPVLPAYIAGMALAGSVAKDEFFIRRFRTLTIGFLTPLYFLRA
                     GYLVSIPAVLAAPLVFLALLGGKVASKILGLYPVIGVFRESKKERWYYTLLMSTGLTF
                     GSISALYGLTHGIVTRDQYSFLIAAIIASAVIPTVIANKAFLPDHLLEAPMADEEIPQ
                     LDRVTDNWEQEEYDMYTKAKGE"
     gene            complement(19388..19816)
                     /locus_tag="PKJ16_02040"
     CDS             complement(19388..19816)
                     /locus_tag="PKJ16_02040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HNV45792.1"
                     /translation="MSTPTCVFCDIVAGTAPARIIMENELSMVILDINPYAKGHCLAI
                     PKRHVPWWHELTEDEVASLFTLAHRAANRIMKTLAPDFVCMYARGKRIPHTHIFLIPS
                     REGDVLDRFYNAMEKFQEAPRDLAKLAEPGTLDEARDLLT"
     gene            complement(19911..19982)
                     /locus_tag="PKJ16_02045"
     tRNA            complement(19911..19982)
                     /locus_tag="PKJ16_02045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19948..19950),aa:Gln,seq:ctg)
     gene            complement(20198..20710)
                     /locus_tag="PKJ16_02050"
     CDS             complement(20198..20710)
                     /locus_tag="PKJ16_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45793.1"
                     /translation="MRNPIALPDVLFTAFALIPPAAGIIAEGINRTPLIIVIVLAILY
                     LAALSIVLMIAVGRLHSRPALVISRCYAIVFSAPCCYISAFTIGLALLLRLFPNCTMV
                     NGDIRCTMATGQIFGAIIIAAIGGSALLHVYYRRFGNLFHRAATALTDGSRHDGDRAK
                     PSSAPSNLRE"
     gene            complement(20793..21887)
                     /locus_tag="PKJ16_02055"
     CDS             complement(20793..21887)
                     /locus_tag="PKJ16_02055"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HNV45794.1"
                     /translation="MTLKKVGILTGGGDCSGLNAVIRAVTRAAAIGHNAAVVGIEGGF
                     DGLVFNRTTELTVKSTRDILTLGGTILGTTNKGNPFEYRELKADGSISVTDRSDETVA
                     NFRKLGLDCLFVVGGEGTLEIGYKLHKKGVPTIGIPKTIDNDLDKTDYTFGYQTAVQV
                     ACDALDRLQTTGKSHQRVMILEVMGRNAGWISMESGISGGAHIILIPEIPFNIDKVVE
                     KIQFREKGGSPFSIIMVAEGAKEEGGEQITLSSASTRLQGVPQLGGVGQYLADQIKKR
                     IDLEVRCTVLGHIQRGGSPCAFDRVLGTRLGSYAVQAAAEGRFGTMVALKTPDMVLVP
                     LEELAGKVRTVPVDSQLIQCAEAIGISMGR"
     gene            22241..22606
                     /locus_tag="PKJ16_02060"
     CDS             22241..22606
                     /locus_tag="PKJ16_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tudor domain-containing protein"
                     /protein_id="HNV45795.1"
                     /translation="MKSAKAIAVILFALGVTAAFAWNVGDRILGQWTDGMWYPAKVAG
                     VQDGKFAVTFDDGDVGVLASAQIKRIDWRVGTKVQCNFRNAGLYYPGTITQMRGEVIH
                     ISYDDGDQEGATISRCRAR"
     gene            22860..24098
                     /locus_tag="PKJ16_02065"
     CDS             22860..24098
                     /locus_tag="PKJ16_02065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV45796.1"
                     /translation="MNDEYRFRTAQSPDDAEKLKNHFDGIFLPEKVGEVAVALYHHRP
                     GMGPENWFIIEEATTGAIASACTLIPWVWEFEGVRLDVAEMGLVGTAAPHRGRGLMNE
                     LARRFAEEMNRRGCDLGGIEGIPGFYARYGYHYAVPLENHINLPLHTCSGMEAGAYAL
                     RTATRDDIPFLMAQDDEYRRAYSLSSVRDAASWDYIFGPSVDTECARDIRIIERDGAP
                     LFSFAMMAYGFGAGLIVCEVSEGITCEALCAMLRFLGEEAARRGKPYVRFNLHVDSSP
                     ARMLIALGAAVNPGWAWQVRVPHPERFLAKIAPALDRRLAGSPFRESTMAFGVNLYCA
                     RYRLALANGRIKSVERDAGECAHAINVSADLFPALALGAHGWRELNRVRPEVCASSDM
                     AGLLADALFPPRRSWMRLAY"
     gene            24234..24356
                     /locus_tag="PKJ16_02070"
     CDS             24234..24356
                     /locus_tag="PKJ16_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016990954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45797.1"
                     /translation="MAKSTDARKDAKKKPEKTMKEKKQAKQQKKNERDRENKSL"
     gene            24489..26123
                     /locus_tag="PKJ16_02075"
     CDS             24489..26123
                     /locus_tag="PKJ16_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV45798.1"
                     /translation="MLEASDVTLSFGKRVLFKGVNIRFTSGNCYGLIGANGSGKSTFL
                     KVLSGDIEPNEGAIIRNPKERLAMLQQDQFAFDEYPVLDTVIMGHRQLYEVLREREVV
                     YTKEDFTEADGHRAAELEELLGSMNGYEAEADAATLLSGLGIDDALHGKLMKELEGGQ
                     KVRVLLAQALFGNPDILLLDEPTNNLDLESVTWLENFLYNFNNTVIVVSHDRHFMNKV
                     CTHIADIDFSKIQIYAGNYDFWAQASQLAVKQKREENKKKEDKIKELQAFIERFSSNA
                     SKARQATSRKKLVEKLTIDEIPTTSRRFPYVAFKPERECGKVILNVENLSMTIDGDPI
                     IRDFTLTVNRNDKIAFVGQNNLVKTVFFEMITGAVKPDGGEIKWGETITTAYFPKENA
                     SFFESDVKLVDWLRQFSDEKDETFVRGFLGRMLFSGEESLKKASVLSGGERVRCMLAR
                     MMLSSANVLILDEPTNHLDLEAITALNNGLIAFPEVVLFASHDHQFIDSIANRIVEIA
                     PGGVIDRYMTFDEYLEDEGVRALRDELYHGHQRLAL"
     gene            complement(26128..26529)
                     /locus_tag="PKJ16_02080"
     CDS             complement(26128..26529)
                     /locus_tag="PKJ16_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45799.1"
                     /translation="MTRYILLWFALLAGAIVNAGIRDFTYGKLLPGNLSHQLSVFPGM
                     AIFGLIIWRACRRWPLASKGRALGIGLLWVAMTEVFEISMIVFLQKKPVEVFIDMHRI
                     WQGELWVLLLLWIAIAPVLFTRTDTTAAAGD"
     gene            26711..27706
                     /locus_tag="PKJ16_02085"
     CDS             26711..27706
                     /locus_tag="PKJ16_02085"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV45800.1"
                     /translation="MNLPPVYAELSKKLMMEHSKLLPELWRMVCDEDEANAVNALPGT
                     AEEIARALGRPVGDMEMLLKRLYHRGAVFDSVKDGTLYYRMPRQVVQFHDATLLWDEA
                     PRELIDLWVRFMDEEYPQLLELVTTVKFPSFMRVIPIGETVAPASSVLSSEDAVAMVR
                     AAKTLAVTRCVCRLSVKNCDKPVEMCLQLNRGAEYTLKRGTGRAVSVDEALAILKKAE
                     EAGLVHMTENRAGMGNAICNCCDCCCEMLRYAKNASTKGVLAPSRYLAAVDAAVCTAC
                     GSCADICPMGAIEVAADSAAVAADACIGCGLCATVCAPGAIMLQGVRPEDFIPAR"
     gene            27815..28672
                     /locus_tag="PKJ16_02090"
     CDS             27815..28672
                     /locus_tag="PKJ16_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45801.1"
                     /translation="MKVDTPKLQNLLSKYPTEFTCNYFSDVVVVGKKLKAGEAVLLFD
                     VNNMVKSGIKDVEVVYDVTMYEYLCREYPSEYRRPVRWLSYFEIDKALEEIEKVNAQT
                     KRKRFLVNIGDVYEKEAKGQKSGAILKHNEKLDYQKWKTVKMHINSEQKFFCRNSEHG
                     IILFMSMKSEGGADYVDKFSKQADLIAAMVSRRTDKNEIAPDFVPTEDVHPVTVPGQL
                     LEEYIRTNARLIIIGENITGPFKEALLQVKSYDPFVRMMVAPPYDPKTVDHFLQQVKI
                     VYNADRWKK"
     gene            28783..29319
                     /locus_tag="PKJ16_02095"
     CDS             28783..29319
                     /locus_tag="PKJ16_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45802.1"
                     /translation="MKIRPPHALVLAALFAMTMATACDRSDSRCRSQIKNLLAECESF
                     IADREEERWKQVSHAVGNVIIREIFLGKRNEHMHIALTIDHFMSAREIAAIGVQIKEK
                     GDFQPFIAIWRDQGLVRKTGREAVVPERIAKVSTAADTVIDLKGSGGGTSIRATVFYS
                     PEAVMSWMQNQKKIFGAE"
     gene            29349..29903
                     /locus_tag="PKJ16_02100"
     CDS             29349..29903
                     /locus_tag="PKJ16_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45803.1"
                     /translation="MSRITNCLRAVIAVLPFAFAAIVACDLGETRSGSMGREISSEQD
                     AAMVLSELGIAPGPTLASIQRHPKSAGTFGREGLRIAAVYRVSRERADAILGTRLAAW
                     RRGPLPAIARRFEQGPKELAAYSPGAFTRCIAWFAETGKPVPCDDPAFAAAFGKNPMA
                     CRHYRAAVLDRNAGTLTVVYKNYY"
     gene            29920..30255
                     /locus_tag="PKJ16_02105"
     CDS             29920..30255
                     /locus_tag="PKJ16_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45804.1"
                     /translation="MERTETRIGVINGILSALHLYSLAQAQGLVMATVMTLGAPASQD
                     SALRTLIGYAVLAHPVVTLVSLVSTWVLFWKGRYLLSLLCALLPVANAIVAGAAILLS
                     VLLGRPIFG"
     gene            30340..30663
                     /locus_tag="PKJ16_02110"
     CDS             30340..30663
                     /locus_tag="PKJ16_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45805.1"
                     /translation="MAELVNAVPGHEVVLLFKRIMWLFSSTVFGKSRGISFVEVNRKL
                     FCVLLILLERIISCEAKFNGCSGEIRNDCGLCSCTPSGRSDYFWLMCGTIGTQVLCVI
                     GNYFC"
     gene            30712..>31275
                     /locus_tag="PKJ16_02115"
     CDS             30712..>31275
                     /locus_tag="PKJ16_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45806.1"
                     /translation="MKKYFFVAVSLVCMVCMTSVAAFADEIDISKIALERLRKTAAAT
                     NAAGQKLVGVKFLSTLETTGIKFKLPSAVPEQPFVLMTQTLSNNLASEARLKCGVEES
                     SMDSETIGTSKTLGGETEVSLSYKSPVGLTVAGKQSFNYSTTKNMEKTTSKTRTWRAE
                     GDFPVGPKERIDVQFVVSEKRLDNIPWS"
BASE COUNT         5962 a         9140 c         9668 g         6505 t
ORIGIN      
        1 ttatacaggt aaatccacga ttgtgcaagc agggggtcac atcatattgt ctaacccctt
       61 ctcccaaagg agaaggggaa atagtatgga gtttattgct gaagagccct ctcctgtggg
      121 agagggcgcg ccgaaggcgg ggtgaggtga cgtgagtttc ttcgaaaaaa catagaatac
      181 catcccaaga ggtgcatcga tgcgtacgaa gtatgtcacg ttcgttcttc taactgtatt
      241 ctccgccctc gcgctttccg gttgttacga acagggttcc ctgtactcga aggtttcgag
      301 ccggacgctg ccggccggcg attttcttgt ccactggtcc aaccccggcg aggggaaggc
      361 cttcgtggcc gtgcggcacc gatccaatcc cgggcgcgtc ctctggtcca ccgagcccgg
      421 gcagaacttt cttcgcgcga tcatggccga catcgacgtg aaggagcacc gcggctcgtt
      481 caccgtgaag gacgacgagc ggctctcctg cgccttccag agcgtggacg atgtgcggct
      541 cgacggggcc gcggtgacga tcaccggccg gctggcggac gcgaaggcga agaacggcgc
      601 ctcgtacgtt atgaccttcc ggcaggcgtc gggcggtcat ctccgcttcg aggcgagcgt
      661 cgcgaagatc ggctgcgcgg gcgagttctg cgcgcgcgcc ttcaatcaga tcgagatcag
      721 ctacgcgtca tcgtctaatg aaaagttcta cggcttcggc gagcagttca cgcatctcaa
      781 cctgaagggg caccgcgttc ccatcatcag ccaggagggc ggggtgggcc gcgggcgcga
      841 gccggtcacc tcggcgctca atctcgtctc gcccggctcc ggcggaaacg cgtacaccag
      901 ctatatctcc gttccgcact acattacctc gcttatgcgt tccctctttc tcgagacgac
      961 cgagtacgcc gtcttcgacc tgcgcgacga cgaatccgtg agcgtgaaga tattttcgga
     1021 ccgcatcgcg gggcgcatcc tctacggcgc gaacccgctc gagctcatcg agcggtatac
     1081 ggaatacgcc ggccgcatga aggcgctccc cgactggatc ggctccggcg ccatcgtggg
     1141 catgcagggc ggcaccgagc gcgtgtacga gatctggaac ctgctcgagg cgcagggaac
     1201 gcccatcgcc gccttttggt tgcaggactg ggtgggcaag cgcaagacca tgatcggctc
     1261 gcagctctgg tggaactggg agctcgatgc gctgacctat cccgagtggg acggcctcgt
     1321 cgccgcgatg cacgaggcgg acatccgcgc gatgggctat atcaactcct tcctggtcga
     1381 tccggtccag aaggggagct tcgcgcgcaa cctctaccgc gaggccgtgg acggcggctt
     1441 cctggtgatg aacggaaaca atcagccgta ccccatcaag aacaccgact tcgacgcggg
     1501 gatggtggac ctctcgaatc ccgacgcgcg cgcgtggatc aagtcggtca tcaaggagga
     1561 gatgatcgct cgcggtttct ccggctggat gctcgatttc gccgaagcgc ttcccttcga
     1621 cgccgtgatc gcgagcggcg atgccgcctc gtatcacaat caatacccgg tcgaatgggc
     1681 gaaaatcaac caggaggcga tcgccgagct tggcctggac gacgagatcg tcttcttcaa
     1741 ccgctccggc tacacgaaga gccccggcgc gagcacgctc ttctgggagg gcgatcagat
     1801 ggtcacctgg gacggcgacg acggtctgaa gagcgctatc aaggggctcg tctccggcgg
     1861 cttctcgggc ttcagtctca atcacagcga catcggcggc tacacctcgg tgagcctttc
     1921 ggttctgaac atcttcaagc gcgagaagga gcttctcatg cgttggatgg aggcgaacgc
     1981 cttcaccgcc gccttccgca cgcacgaggg caacgagccc gaaaggaacg cgcagttcta
     2041 ttccgacgcc gacacgctcg cgcaattcac gcgcttcgcg aaggtgttcc gcgcgctcgg
     2101 cttctaccgc gccgagctga tgcgcgacgc cgaggcgaag ggctggccgc tcgtgcgcca
     2161 catgctgctc cactacccga acgatcccgc cgcgtacgac ctgaagtacc agttcatgct
     2221 gggcgccgat ttcatcgtcg cgccggtcgt ggacaagcgc gccgattcgg tccgctgcta
     2281 tctgccggcg ggggagtggg tccacgtgtg gaccggggct tcgtataacg ccggatgggt
     2341 tgatgtgggc gcgccgatgg ggaaaccggc ggtgtttttt aagaagggat cacccgccgg
     2401 gagtcggttc gtgcggaacc ttcgtggtga gggagtgctc tgagcattcc gacctcctgc
     2461 ccgcgtcggg catgtccggg atgggacagt tgtcttatgg ccgtacatgc tcacgcgatt
     2521 ttctcccggg agtggagaag gttttaggag aatcgaacgg cttgcgatcc tttcatggcg
     2581 tcaaccgccg ccacctgccg gtttatgagg attgcgcgcc gcaccgcatg tcgcgattgt
     2641 cgcacgcatt cgcattcgaa taccgcgctc actcgaacat ccccctgaag gacgaatagt
     2701 aatccagcat ctcgccgttt acgatctcgc gctgttttag aatgatgccg aacgattgga
     2761 tcaggtggcc gatctcgaac cagaaggtcc ccgtgctgct cgtggtgaag atgtccttgc
     2821 ggagcagcga gtcgatctcc tcggtcatgt tccatgcggt gaactgcgcc gcgggccgga
     2881 tgttctcgaa caagtacgtc cccacgccgt ccgggctgaa ggcgaccagt ccgagctgcg
     2941 ccagttcatc gtacagcgtg tccccgtact gcatgtagac atcgccgtcc cgttcgaccg
     3001 tgagctggta tttccgcagg agcttcggca ggtttgccgt caggttggtg aacagcctcg
     3061 agtagcgatc gtcgcgggtg cagggtcggc agttctcacc ccgatcgtcg caggtctggc
     3121 aactctcgtc ggaattatac agcaggctcg cgacggccga tcggaccttc acgagatcgt
     3181 cggttgtgat gtcctggatg ctgggcgagt tgatgaacgc gttccaggcc gtgttgatgc
     3241 gcccggtcag gaagtgcgga ttcgtccgat attcctcgtc gggatgatcc gggtcccccg
     3301 ggttgagcag tatcgcgatg atgtcggcga tattgttgag cttgtggcga tggcgcgcgc
     3361 ccgccggctc ctgcgccacg taatgcgtaa cggcgccgtt gcccgggttc atgtcgtagt
     3421 cgtatccatc gtcgtaggcc ttctcgatca tcacgccgcg cgacttctga atgactgtac
     3481 ggatgctggc ccgatggttc gtctcgtccc ggtaatccgt ggacgtgaaa tatccccgga
     3541 ggagctccgg cacctccatc tcccgctcct cgtagacgga gtggatgatc gagtccttcg
     3601 cgtaatcaag ctcggtctcg atatcgagtc cgtcgcccca acggtagagc gtgtcgatga
     3661 aggtcgtggg gcgatagtac cggtgcggtc ggtcgaatgc cagcggcatg ccgttctcgt
     3721 cggtgatgcc cgccgtgaag tcggtcagga cccgcgacgc ccgcggcacc gtggcgaaat
     3781 cgcccgccgc gaacctcgtc gcatcgccgc cgaagattcc ctccatgttg atgtcgtact
     3841 tgtgcggatt gaacacgttc gggcaatcga cggttccggc gcgataccac gggtcccggt
     3901 agttgtcgca gagctcgctg tacgcgttcc ccagcgtgcc gtcgttcctg aagtcgaagt
     3961 acgcggtgat gccgccgaag tcggtggaat agtgctcatc gtcaagcacg cccgagggca
     4021 tcatgatcct cctgatgtcg atgtagcggt aataccgaag cagcgcgccc tcgccgtcgg
     4081 tgaaatcaac gtaggttacg tccgccggcg cttcgccgtt ctggtactcc gcttgggtga
     4141 tctcgacgta cgtgccgaaa ccgtagattg tgtcgccccg cttgaagtag cgcgggtccg
     4201 gcaccacggt ccccgcatcg ttccggagtc gatagatccc gtcgatcagc ccctccttga
     4261 ggttgacccc ggccaacttc accagcgtgg cgttgatgtc gtcgatgttc ttccggacat
     4321 cgctcatcgt gagtccggtg gcgtccaggc tccagttgtc gaagctgaag ttgtagagct
     4381 tctcgatgtc gttgatgccg ctgcgtttga tgaagtcgat aatctggtgg tagtcggtct
     4441 caatgagcac gtccgccgcc ttctggatat ccgcggccag ttgcgccgcc ttctcggcgt
     4501 cgtagttcgc cgctcccggc gccagctggt tcccggcgtc gtccaggggg gtgtagagct
     4561 gtaggacgcc gtcgttgagc gcctccacgc gcgtcatctt gacgcagccg gcggtgcagg
     4621 cgtcgtaatt cgcccggcag gccgcccggg ccggttcatc ctcgatcccg gagcagtcgc
     4681 acgccttgac gcacgttcgg tactgctcgt cgatgtgctt cacgatcagg aagttgtcca
     4741 gcgcgatgat tgtcttgcgc agcatctcct tgaactcgtc gctgcgcgag acgtcccgca
     4801 ggaggtccag gctctcctcg agccgccgca gcgattcgtt gtcggtgaag tatctgagct
     4861 tgttgatggg cgtctcccag ttgtgccggt tgctcacggc ccgggtcccg tcctcgcgcg
     4921 tcacgtaata cgggagcggg gcgtccgcgc cggtggcggt gagccggaag ttccctccgt
     4981 tgttgaggaa gttgcgcaca acgttcctga cggccccctc gacgctttcc ttgatgggat
     5041 tgaggttgtt gagattggcg tacgtgctct cgatgacgta cgagagcacc ccgttccgcg
     5101 cgccgatcgc gtcgcccgcc atgccgcccc ggtccaccag gaggttcaag agcgagctcg
     5161 ggtcggggat gggcgcggtc ccgcccggtg cgttccgcgc ctcgttgagg gcgcacacgg
     5221 tcatgacgaa atcgttgatc tgcgtccggt agccgtcgcg gtcgtagtag gcgatcacgc
     5281 ccctgtcgat tgcgctgtaa tccgccggga cgacgttccc gacgccgtcg aaccccatga
     5341 tccggccgtc ctcgtgcaac gtcccctgga tggcggcgta gagggcgacg aacaggtcga
     5401 acttcccttc cgtctcgccc tcgtagacac gccaggcggt cgcctgaccg ctatcggtaa
     5461 atgaggtcgg ataggaaacg ccggccacgc gcgggatgcg gggcaggtcc ttctccttga
     5521 tgctctgctc ccttccgttg tcgtcccggt agatcagcgt gccggccgtg gcgcccgggc
     5581 ccacatagga catcgtgtag tatgtccgat agaactcatt gaatttcgtc attgcgtcgg
     5641 atgtcggcga cgcagccccc aggacgtccc gtgcgctgta gacgctgtcg cccagcgtga
     5701 tcaacgcctg cagattgtcc ccgatgaccg ccgggaggat cgagccgttt ccgacggccg
     5761 accagagcgc atcgacgatg ctcagcagca tgctgaggat gccggcggac atgttgtcgg
     5821 gaagcgggat gaggtcgaac aggagggtga tcttcgacga gtagtcgtat tccatcgcca
     5881 cgcagaaatc ctgcggccgg aagctcgacg ccgccattct gaaggtggca ttggactcct
     5941 cgatgagctt gaccggatag gtgggattgc cggcgtcgtc gttcaccacg tggttcgaat
     6001 cgatggccat cgtattgtcg tacacgtgct cgatgccgcc ggaccacacc gcgttgttgg
     6061 tcttgtcgtt cggcgccgcc tggtggcgtc tcccgcgggc cgcgccgccg atgccgttga
     6121 aattgatggt cacgtaggtg tagatctccg tcttgatgtt gcacagcgac ttgttgatga
     6181 gcgggatcgt gatgatgtcc ttgtcgagca tgccgtggat gggcagcagg tatacgtact
     6241 tcttgtagtt gagcagccac tggaagttct tccgcattgc ctcctcgcgg gtggcgcagt
     6301 cgagcacgac ctgtcggtca tacgtccccg gatcgtcggg attgacggcg ttcgcatagc
     6361 tcccgtcctg ggtgatgttc atgccgaccg ggctgctttt acggaaatat ccgtaccgcg
     6421 agttgtgcgt cggcttgtag atcgcctcga aatagtggta tcggccccgg tcgtatttga
     6481 ggccgggccc cttgttcccc cattccatcc cgatcatctg gtaccagtcc gtgtaccagt
     6541 cgttccggta tttgcactcc gacccgttgg gggccttgcc gaggtacgtg taggggccgt
     6601 agccctccca gcaggccagc gcgatctccg aattgagcca gtccgacgtg ttcaggacgt
     6661 cattgccggt ggctgggtta tgccgggtta tgccgaagag gtcgccctgc gagggagaaa
     6721 aaacgccatg caacgccgcg cccggattgt tgctccccac gcgctggcgg aaggtccccg
     6781 gctgcagcac ctccatgacc ggcatctggt actgcgagga gaaggtcgtc cacgacggcg
     6841 gggcggtgtt gggcctgatg gacccgctgg cgatatgata cccataggac gggctccgtc
     6901 tgatggtggc gtcctcgagg caatacaccc gccacgatcc gaacagctcg agcagccaat
     6961 cgatcgaatc atccacgtag tccatggcgt tgaggtagtt ccgggagcgc agcggcgagc
     7021 cgagcgagtt caggacgttc cgcagcgaga gttccgtctt cgcgttgttc ggatcgacca
     7081 tgcccccgcc ggcggcgaag agatagacga agttggtcag gagcgacagg ccgtttcccg
     7141 tgtagccggg aacccgcgga tccttgccgt cccggtccag tatgaccagg ttccggatgt
     7201 tggtggtcag ggtgccgaag agggtctgca ggttgcgatt gggatcgccg aaaaacgcct
     7261 ggccgtcctc ggggccgaac gcccacttgc cctcggacgc cttgtaatag ctcgacagcg
     7321 tatagaactt caggttggtc atgacggact cgatgatgtt ctccggccgt ctgtcatttc
     7381 cagtgcttga atacgacagc cccaccgtct tgaaagtccc gttggcgtta tcgtcgtacc
     7441 cgccgaggcc gtcgctgttg aggatgtcgt tcacttggcg acggaacggc ttcaggagcg
     7501 catcggagct cagcacccgc cccagggccg tcacggctcc gccggggctc accaggccgg
     7561 ggaagaatgt ggacgcgccg gcggtcgggg aatacgtgtg gccgttccac agcaagtcgg
     7621 tcatccggtc ctggggaaag ttcgccagca ccgtgttcca tcggggcacc acgccgcgcc
     7681 gcgccgcatc acccttgagc cagcgcagga tgtcgttgtt cacgaaatcg aagaggggaa
     7741 cgtcctcgag gcggtcgtac cagtccatgt cgtgatacag cgcccccagg aaccatttgg
     7801 aatacaggcc gtcgtcgttc gattgcgccg cggcgtccgg cccgatgttg tacttgcgcg
     7861 agcggcgctt cgctgattcc gaaagataca gcgattcgtc gttgaggaac ttcttccccg
     7921 attcgagcac gtcgatgctt cgctcgaccg cgccgtcggt gacgatgtcg aagacgggtc
     7981 gctgcacctt ggggaagaga tcgttgatga gatactcgcc gatggagtac ttcagcggat
     8041 cggcgccggg ggccatccaa tccgcgatct ccttctcgat ggcggtgatt ccggtgaacg
     8101 gatcgttcgt ttcaagatag tcgatgaagc tgaacatctt ctgctcgatg tcccagtgct
     8161 cttcgatcac cgactcgatg ctttttcgga tgatggtgga caggtcctgg agcccctcgc
     8221 cgtctccgta cagcgtacgg gcgaagctga tgttctcgat cagggtgttc acgcccgcgt
     8281 acccgatgct ctcgagttcc tgtccctgtc cgaagaggcg ccaggggtag gccgacgtct
     8341 cggcgggatc gtaggcttcg gcggaatcga acagcgcatt gccgttcgcg tcgacccagt
     8401 agccgccgtc ggaattcacg tcgtcccagt ttcgcaacgc gacaagcagg tccatgatga
     8461 ctcccgcgcc ctcggacatg acggtcggat cgagatcgag gatcgccttc acgatgtcga
     8521 cggtggtgtc gagcgagtcg gggtcccgca tgtacgcgtc gtggaacgaa ttgaaacttt
     8581 tcgacagctt gtccaccgtt tcggggaaaa tcgcctttgc gccgggggtt tcgggcaggt
     8641 agagcgcctc gccggtgcgg accatcagct cgcccagcag gtcgggattg gcgaacatca
     8701 ggtcgttcat tttccggttg aattctcggg gctcgagatt gctgaaggcg gacttgagcg
     8761 cgggagattc gttgagcacc tcgaagagca tgaccgtatc gttcggctcg gtctgctgga
     8821 atgtctggtc gccgtcagtt ttcgaacatc caaaagcgaa aaaacccgac agtatggctg
     8881 ctgtaaaaag cagtgcgatg cttttcatcg aatccccctc gatatccgga tgaagaacaa
     8941 atttcgaaca gatctgagat tttttattag agtcgttgca aaacaggatt tcctgtcatt
     9001 gcgaggagcg atagcgacga agcaatccgg cgctttcgat ggaaagccac ggtattgggc
     9061 caggtgctca tgttgcacgg gttgtaccgc acggatcgct tcgctcgcga tgacatcgag
     9121 ttgcacaaca agtctatgaa aactgcgccg aaaagaatca aaaccgaccg gctggtagta
     9181 atatgatgtt tttttatgat cccgtcttgc caataatggc aatacccata tttggcatga
     9241 gattgtcgcg agcgcatcat gcgttcggaa cgtcaaacga gagccaaaca caggcgacct
     9301 gtttttgtca tggcgcccgt cggggcgtcg ggggtcagag cctcatggtt tatttttctg
     9361 caggtggaag caaaaaaatg gattgtcagt attgtggtga tggtttcctg ttatctcaac
     9421 gcgcggcgat acagagaacg tcgcgtccgc acactgcaga taacaacgag atgacacgtc
     9481 gggataacaa catgccagat ctcgcacgcg cccgccccgg cagagcgcgc cgaacggttg
     9541 tgatctgtgc gctctctgcg ctgatcgcgc tcgcggcggg cgccggtccc ctgcgggccc
     9601 cgcccaacat cgccaccctc gccgagcgca actcgctggc cgacgaatgg cggataaccg
     9661 cgtcggatcg cccggagtat tcgtcgcgcg catacgacga ctccgggtgg gacacgatcc
     9721 ggatgccggg aagcttcttc gggtacgcgc tcagaaagac cggagcgctg tacgggaccg
     9781 tctggctgcg caagacgatc atcatcgatg cccgcgcgga gcgactgccc gcagtgggac
     9841 tcatcctcgg gcgcatcgcc aatgcagacg agacgtattt caacggggag cggatcggcg
     9901 ggatgggccg gttcctgccc gaggacaatt ccatgtggaa ccatccccgc cactacctgg
     9961 ttcccggaaa gcttgtcaac tatggcggga aaaacgtgat cgcgatacgg gtgtcacact
    10021 atattttcgg ggaaatgatc gggaccctcg ccctcactga tctcgatacg tgggaacagg
    10081 accgggtatg gcagaacttc acccgggtga ccgccggtta tatcatcatc gccatgggat
    10141 gcctctttct tgtcatattc ctgctgttct atgcgctccg cagggagcag gaatacctgt
    10201 actacgcgtt gcagatggcg tgcggctttt tcatcgtact cgaactctgc aatttctgga
    10261 acaccttcgg aagcaacctc atgcggctca aggccctggg actctcatgg gccgcggtca
    10321 atgtcgtgca cccgattttc ctgcaccgga tctacgatct gcagagaaaa aaaatcgaat
    10381 gggcgctctg ggtgttcctg gcgctgacga tcggactctt gctcacgatc acgggcaagc
    10441 cctccgatct gctccaggtg aacatcttca tcaccttcac catctgcatt ggcctctata
    10501 acatctcctg ccatgtttcc gccatcttca agaggcggcc ctattcgcgt gttttcggtt
    10561 ttttcgggat cgctgtggcc ctgggggcca tacacgacgg gctggtgtac ctggccaagt
    10621 tcaccgacta cagcatatcc ctcttcgggt atgtcttccg ggattacatg atctttcatt
    10681 acatggccat catgctctat accgggaccg cgctggtgct ggtgttcagg ttcatcagca
    10741 tggccaccga ggtggaggac ctcaagacga cgctcgagaa cttcatcatc gagaacgcga
    10801 tcctcaataa gcaggtcgtg cgaagtcgtg acgaagggaa aaagcgcccg gaggaatccg
    10861 cgctcaccga ccggacgatg gagaagctcc agaaggtggt cgccttcatc aatgagaact
    10921 accagtcgag cctctcgcgc gagggcctcg ccgcctcggt cgacgtccat ccggacaacc
    10981 tgggcaagct cttccgtcgg tacaccagca aaaagctggg cgattacatc tgcgagctca
    11041 gagtcgagga cgccgcgcgc aagctcgtgg agacagacga acccgtcatc gacatcgcct
    11101 tcgcgaccgg cttcgagagc ctgcgcacct tcaaccgcgc gtttctgaag ttcaagaaga
    11161 tgactcccgc cagctacaag aagaagaaca agccggcgac gacatcgtga ggcgccgcgt
    11221 tcccttttcc agccgctcca gctgattgac gaccgatcta tcggacaggg gccgggtgat
    11281 cgctttaagc ccctcatcgg caaggacaaa ttcggatact gtccattatt ggccggaaag
    11341 cccatgccgg attggataat attaatccat cgggactgtt atttgtcatt ccccgatggt
    11401 ttagatttct catgtgagta tcgcaaacaa tgtaatcaac atatagagtg cagggtgttg
    11461 gcaaaccggt cgaaccggga gcgccgccca tgcgaaggaa cagcgaaatg aaggtgtcca
    11521 ggacgaaacc gctcatcgtt attttccatg gtatactgat cgcggcagca gcgatgtttg
    11581 ttcatgccgg cccctcgttt gcgatcgaac cggtcgtgct cgacgagacg cgggaagagt
    11641 acatactggg aaagcatatc gagtatctga aagatgcgaa gaaagaatac gcgattcagg
    11701 atgtttccgc ggccggattc gattcgcgat tcaccgccag caccgaggac tggccgaatt
    11761 tcgcatacac ctcggcggcc tactggctgc ggttcacgat caggaacgcg tcgaaggaga
    11821 cacggcgatg gatccttctg gataactatc cgctgactga cgacatcaag cttttcatgc
    11881 cggcgcagca tgggggcaag atcctgaagc acgcggggcg ctttctgccc tatgcggcga
    11941 gggacgagga ctaccgggcg ttcgcgttcc atctggacgt tccggccgga tcggaacaga
    12001 cgtactacct gcgcctcgag agcgaggatt ccctcgtggc gatcctgtcg atcgtgtctc
    12061 cgaagcgctt cacgcagatc aagcacaaca cccagatcat gctggggatc tactacggct
    12121 ttatcctggc gatgatcatc tactatttct tcatttttat cgccacccgt gaccggaatt
    12181 atatctacta cgtgctcacg ctgatctttc tgcatctctt gtttcagttt tcactcaacg
    12241 ggctctcggc cgaatatatc aaccaaacct cgctctggtg gtcgcgcggc tccatcgtat
    12301 tcttcgaggc catcggcgtg tttttcgcca tgcaggtcgc gaaggaattt ctgcagacga
    12361 agcggaacgc ccccgtcttt cacaagctgt acaatatcgt catgtacatc gtgttcgcca
    12421 tcgcgctcgg gtcgctgttc ctcccgtact actatatgat catctcggcg gttctgatga
    12481 ccatgagcgg gtcggtgctg atgtggtcgg cgggcctgta ctgctactat aaaggattcc
    12541 gggcggcccg attctacctg ctggcctgga cctccattca gctcctcggc tgcttctatg
    12601 ggctgaaaac cctgggcctc gcgccgagct tcttcctgtc ggaatgggga ttccagatcg
    12661 gatcgctgac gcatgccctg ctgatgtcat tcgccctggc ggacctgata aatatcatgc
    12721 gcattgagaa gaccacagcc ctcaaggaat cgatttcgct aaaggaagaa atgaaccgaa
    12781 acctcgagat caaggtcaag gagcgcaccg aggagcttca gcaggcgatg gccacaatga
    12841 aggcaatgaa cgaggagatc gtcaccgcgc gcgacgcgtt gtggggggag atgcagctgg
    12901 cgaagaagat ccagacggtc ctgctccccg tcaagcccca gatgaaaggc tacgacatct
    12961 ccgtgttcct caaaccggcc gatgacgtgg ggggcgatta ctacgacatc atcaatgtcg
    13021 agaacatcga ctggatcgtc atcggcgacg tgtccggcca tggcgttccc gccggcctaa
    13081 tcatgatgat ggtccagacg gcgatccaca cggtcctcgc gagcaagggc aacctgaaac
    13141 ccgaggcgct gctgaccagg atcaaccggg tcatcacgcg aaacatcagg ctcctgagcg
    13201 aggacaaata catgaccctc acggtgttcg cctgcatcaa ggacggcaag ttctactact
    13261 cgggcctgca ccaggacatc atgatctatc ggagtacgga aaagcgggtc gggctgatcg
    13321 gcaccaacgg catatggatc gggctgctcg aggacatcag gggcatggtg accggcgagc
    13381 acttcagcat ggcggtgggg gatgtcatga tgctgttcac ggacgggatc accgaggcgt
    13441 gggttgaggg cagcgttcgc gacaaccgca ccatggagac cgacatgttc ggccaacata
    13501 agctcaagga ggtgttcgag gaatatggcg agcaccatcc cgatgacatc aagagcggca
    13561 tcctgagcaa gctggatgga taccggatca cggacgacgt gaccatggtg atcgtcaagc
    13621 gccgggaatg atcggtcgac gctcagggaa aacggcagcg gatcacgtaa ttctcttgct
    13681 ttatcgtcac gcccggtagt agtatatacc cggacagcgt ctggcgtccg gaggccccgc
    13741 ccccggaggg atcatgccgt gaagcgatca tatcagctca tcgtcgccat cgtattcctt
    13801 gacctcgcga tccgcctggc gggaggcgat gccgttcggc agatgggcca ctgttcgcct
    13861 ggtgccacaa cggcgccggc atcgcaacag ggcggattgt gcgatgaaaa agcttgtaga
    13921 aatactatca gttgattcgg tgcatattat cgccctcgat gcccgcggaa atcgagtcac
    13981 gatagccatt gccgactgca tcaacaattt ctatcgtgag ttcatgggtg cggagtatgt
    14041 cgtcaccggc aatgaccgca tgatcgggga gcggaacagc caggccgatc cacggtattt
    14101 tatttttcat tccaatccgc gttattgcat cgaagttgat cgcgaccgct ttgatgcgat
    14161 cagtgacatg ctttccggaa ataacctgca gacctttgat caaacatagc ccgtgaatat
    14221 cgttttcgac atggacaata cgctggtgga cgaattcggc gcgtctgcgc gaccgggcgt
    14281 cattgatctg ctcgtccatt tgaaacgaac ggggcacacc ctgatcctgt ggacaaattc
    14341 aagaaaggag cgggcccgtc aaatcatact ggacaacaat ctgcggcggt atttcactac
    14401 cttcatcttc agggaggact acgatccgga agaaaagggg ctgtccaagg atatccggaa
    14461 agtcaagggg gatatcctcg tcgatgatga tccggcggag atccggcatg tcgcaaccct
    14521 cggaaaacat ggctatctgg tagcatcgta ccgtaaaggc atgacggttg acaggaatga
    14581 attcgcgggc ctcctgaaga tgctgcgtta aacggaaacg gatgcggagc acccggcatc
    14641 cggtcgtcgg cccgccgata ctgcgtatca tcttcgccat cttcgtcgag gctgcgatct
    14701 tccgctatct gccgggcgtc tccgtccgtc gcgcgctgaa gacatcaacc attgcgaacg
    14761 cggcctccct cgttttcggc ttgatattta tcgccatgat ctgatccgct ctttgcacta
    14821 cgattcagga gaacgcgcat gccgtcgctg atgcaccgac gatcacatca ttccatgaag
    14881 cgcttctacg attgggtcca ccgctactac ggcagtatcg aagagtcgat cggtccggca
    14941 ttggattcga tcatcgaagg catgccgtcg gtgttcgccg acgccggatc caagaccgcg
    15001 ctcgattacg cctgcggatc ggggctcctt acgctgaagc tcgcccggcg cttctccgtc
    15061 gtcgacggac gagacgcgtc gacgggcatg atcggacgcg ccatggagcg ggcgaacgcg
    15121 ctttcggtct tcaatgcctc atttcgcgcg ggggacatca tggagccgga tgaggccccc
    15181 gggtcctatg atatcgtgtt cgtgtctttc gcccttcatc tgtttcatcc cgatgtggag
    15241 cgggagatcc tgaggaagct gttcgccatc gccacggagg cggtcgtcgt catcgaccat
    15301 ggacgcagat ggagcgcaaa aaccgcgctg atcgaatgga tcgaggggag ttattacgac
    15361 cgtttcatca cgctcgattt taacgcgatg gcgaacgaaa tcggcgcgtc cagattcgtc
    15421 gagacggaga ttgcggacag ccttgtgctt gagttttgca aatagcgggc attggagttg
    15481 cctgtcgcct gtggcataaa aaggcgccat gcgcatttta tgcgcatggc gccttgcgga
    15541 tcacgatgtt cgtttgttca gcgcgcctgc tggatcgcca cggccacggc cacggccacg
    15601 gtcgcgccca ccatcggatt gttccccatg ccgatgagcc ccatcatctc tacgtgcgcg
    15661 gggacggagg acgatccggc gaactgcgcg tccgagtgca tgcggcccat ggtgtccgtc
    15721 atgccgtagg aggcgggacc ggcggccatg ttgtcgggat ggagcgttcg gccggtcccc
    15781 ccgccggagg ccaccgagaa atatttcttc ccgagatcga cgcactcctt cttgtaggtc
    15841 cccgcgacgg ggtgctggaa ccgggtcggg ttcgtcgagt tgccggtgat cgagacgtcg
    15901 accccctcgt gatgcaggat cgcgactccc tcgcggacgt catccgcgcc aaagcagcgc
    15961 accttcgcct tttcgcccga ggaataggcc gtttccttta cgatggcgag cgtgccggtg
    16021 taataatcga atttggtttg cacataggtg aagccgttga tgcgcgagat gatgaaggcg
    16081 gcgtccttgc cgaggccgtt caggatcacg cgcagcgggt tttttcgcac cttgttggcc
    16141 gatttcgcga tgccgatcgc gccctccgcc gcggcgaatg attcgtgccc ggcgagaaac
    16201 gcgaagcact tcgtctcctc gcgcaggagc atcgccgcga ggtttccgtg cccgatgccc
    16261 accttgcggt cgtcggcgac cgaaccggga atgcagaagg attgcaggcc ttgacccagt
    16321 atttccgccg cttcgggggc ggccgtcacc ccctttttta tggcgatagc ggcgccgacc
    16381 gtgtaggccc agcaggcgtt ttcaaagcat atcggctgga taccccgcac gatcgcggcg
    16441 acatcgatgc ccttcgcatc gcagatggat ttggtttcct caattgatgt aatgccatgc
    16501 gcgccgagca cggcgttgat ctgcttgatt cgtctttcgt agctttcaaa tagtgccatt
    16561 gcaatcaccc ttatgttacg cgtgacgagg atcgatcttc ctggccgcat cgtcgaaacg
    16621 gccatatgtc gatgtcgctt ttttcagcgc gtcgttcgca tcggttccct tcttgatcat
    16681 gtccatcatc ttgcccaggt ggacgatttc gtagccgata atctggtcat ccgtgtcgag
    16741 cgcaagctgg ttgacgtagc cctcggcgag ttccagatac cgcacgccct tcgctttcgt
    16801 gctgaatatc gtgccgatct ggctgcgcag acccttgccg aggtcctcga gtcccgcgcc
    16861 gatggggagc ccgccctcgg agaacgcggt ctgcgtccga ccgtaggcga tctgaaggaa
    16921 gagctcgcgc atggccgtgt tgatcgcgtc gcagacgagg tcggtgttca tcgcctcgag
    16981 gatcgtcttc ccgggcagga tctcgcccgc catggcggcg gaatgcgtca tgccggagca
    17041 cccgagcgtt tcgacgagcg cttcgtggat gatgccgtct ttcacgttga gcgtgagctt
    17101 gcacgcgccc tgctgcgggg cgcaccagcc gacgccgtgc gtaagtccgg agatgtcctt
    17161 gatttccttg acctgcaccc atttcccttc ctcgggaatg ggcgccggtc cgtgatcggg
    17221 gcccttggcc acgcacatca tttcttcaac ttctgctgag tatatcatac ggcgtactcc
    17281 tttatggaag ctgtagatac cattgagatt tcaataaaat aattcaagac aaaaaaatca
    17341 cttccatgag cattgtccgt atcatgtgat gatcgaagaa ggcgcaatga agcgggaata
    17401 tatcgataca gcattatcgc atttcgatga tggatgtgcg tgctctcaat ccattctgct
    17461 cgcattttct gaagtgttca accttgatga gacggtcgcg aagcggatcg cttcgacctt
    17521 cggcggcggg atggggcgat tgcgcgaaaa atgcggggcc gtcaccggcg cgttcatggt
    17581 cctcgggctc gcattcggga acgtcgatcc gaaggacatg aagacgaagc tgtatgccta
    17641 cgagaaggtg cgcgaattga atgcgcgcgt ccagggcgag ttcggaacga cgctgtgcgc
    17701 ggaattcttg cgcaaaaagg cgagcgcggc cgatatcgcg gcgagggcgc accatcgtat
    17761 cgtgtgcaga tcgattattg agtacactgt gggcgcgctc gtcgatattt taatcgagaa
    17821 cggccgtctc tctcgggata gctaaccgcc tgcctgatta gatgcgggga gttcgcgccg
    17881 cggcgttttc cgaaggggcg cggtgcacac aaacggaagc cggagaaacg gcgaacttcg
    17941 ggatcaggcg ccatgccgta aatttttcct tgcaccattt gcgcaggtgg ggttgattgt
    18001 ccacgctgca ggggatgggg tcccccgtat aaccgccacc ggctgatgac tcctgccgca
    18061 ccgacgtgtg gcgggtgcct ccagtgaaaa aggtccgcca tgtgtcaggc ggattttttt
    18121 ataccttcgg aggactccat gctcgagtta ttcaccatcg tgaccgcgtg gctcgccctt
    18181 gcggtttttt ccaccattct cgccaaccgg ttaaaaatct ccatggcgct catggaaatc
    18241 tgcgtgggcg cattcgccgg ctatatcgcc atgcgatttt ttgatcccga catcctgcac
    18301 cccaatgcgg agtggatgaa gtttgtcgcc ggatccggcg cggtgctgct cacctttctc
    18361 gccggcgccg agctggaccc ggcctcgatg cgcgcgaagg cgaaagaggt cacggtcgtg
    18421 gggctcatcg gcttcttcgc gccgtttatc ggctgtgcgg cgctggccta tttcctcctc
    18481 ggctgggacg ggcgcgcgag ccttctcggc gggatcgccc tctccacgac gtcaatggcc
    18541 gtggtctacg cggtgatgct ggagtacggc ttcaataaga ccgaatacgg caaggggatt
    18601 cttggggcct gttttatcaa tgacctgggg accgtgattg gcctggggct catcttcgcg
    18661 ccgtttacgt atcggacgtt gatattggtt gccgcatgcg ttttgatcgt cttcgtgctt
    18721 cccccgatca ccgatcgcct cacgaaggtg tatggcaatc ggaccgccgc catccgcacc
    18781 aaatggctgg tcttcgtgct cttcgggctt ggggcgctcg cgctgtggtc ggggtcagag
    18841 ccggtgctcc cggcctatat cgccggcatg gcgctcgcgg gcagcgtcgc caaggacgag
    18901 ttcttcatcc ggcgcttccg gacgctcacc atcggttttc tcacgccgct gtattttctg
    18961 cgcgccggtt acctggtgtc gattcccgcg gtcctcgccg cgccgctggt gtttctcgcg
    19021 cttttgggcg gaaaggtcgc atcgaagatc ctcggcctct atccggtcat cggggtcttc
    19081 cgtgagagca agaaggagcg ctggtattat acgctcctca tgtccaccgg cctcaccttc
    19141 ggctccatct cggcgctcta cgggctcacg cacgggatcg tcacgcgcga ccagtactcg
    19201 ttcctgatcg cggcgatcat cgccagcgcc gtcatcccca cggtcatcgc gaacaaggcc
    19261 ttcctgccgg atcacctgct ggaggcgccc atggccgacg aggagattcc gcagctcgac
    19321 cgcgtgacgg acaactggga gcaggaggag tacgacatgt acacgaaagc gaagggagag
    19381 tgacgcctta tgtcaacagg tcgcgtgctt cgtcgagcgt ccccggctcg gcgagtttcg
    19441 cgagatcgcg cggcgcctcc tggaactttt ccatggcatt gtagaaccgg tcgagcacgt
    19501 cgccttcccg ggaggggatg aggaagatat gcgtgtgcgg gatgcgtttc ccgcgcgcgt
    19561 acatgcacac gaaatcgggc gcgagcgttt tcatgatccg gttcgcggcg cgatgggcga
    19621 gggtaaacag gctcgccacc tcatcctcgg tgagctcgtg ccaccagggg acatgccgct
    19681 tcgggatcgc gaggcagtgt ccctttgcat aggggttgat gtcgaggatg accatcgaca
    19741 gctcgttttc catgatgatg cgcgccggcg ctgtgccggc gacgatgtcg cagaatacgc
    19801 acgtgggtgt gctcatgggc ccttcctgtc ggtgtgattg catacgtatt tccgggcgat
    19861 ggcgggacgt caatcgaatt atcgcccctg cgggcaattg gcgcggcgat ctgaggcgca
    19921 aggattcgaa ccttgggatg ctgggatcag agcccagtgc cttaccgctt ggctacgcct
    19981 cactgttttt caagtctgcc taaccgtcga acttccgcag cgtgtcggtt gtcgatcgct
    20041 gtgaaagtcc cacgctcaat gagccaatat ggcctcgtgc ggagagcatg tcaatttttt
    20101 aacgaaaaat agaagcgttc cattgtggaa tttacatgga tgtaacagcc ctgaatgatg
    20161 atcgcgcgta ttcccgcgag aatcggtttc tggcgtgtta ctcgcgaagg ttcgatgggg
    20221 ctgatgaagg cttcgcccga tcgccatcat ggcgcgatcc atcggtcagc gcggttgcgg
    20281 ctcgatggaa caggttgccg aatctgcggt agtagacatg cagcagcgcc gatcctccaa
    20341 tggccgcgat gatgatcgcc ccgaaaatct ggcccgttgc cattgtgcag cggatgtcgc
    20401 cgttaaccat cgtgcagttc gggaagagcc gcagcagcag cgcgagcccg atcgtgaatg
    20461 cgctgatata gcagcagggg gccgagaaca cgatggcgta gcagcgtgat atcacgagcg
    20521 ccgggcgcga atgcaaacgg cccacggcga tcatgagcac gatgctcaat gccgcgagat
    20581 aaaggatcgc gagaacgatc acgataatca gcggcgtccg gttgatccct tcggcgatga
    20641 ttcccgccgc cggagggatc agcgcaaacg ccgtaaagag aacgtccggc agcgcaatgg
    20701 gattccgcat aacggattat cggggcgccg cgacgcgcgc gtgcggtgtt tcgcgatgcg
    20761 gcgtcggcgg tcgcttcgcc gtcatcgtcg catcatcgtc ccatgctgat cccgatcgcc
    20821 tcggcgcact ggataagctg cgagtcgacc ggcacggtgc gcaccttgcc cgcaagctcc
    20881 tcgagcggca ccagcaccat gtccggggtc ttcagcgcca ccatggtgcc gaagcgtccc
    20941 tcggccgccg cctgcaccgc gtagctgccg aggcgcgttc cgaggacgcg gtcgaaggcg
    21001 cagggcgagc cgccgcgctg gatgtgtccc aggaccgtgc agcgcacctc gagatcgatc
    21061 cgcttcttga tctggtcggc caggtactgg cccacgccgc ccagctgcgg gacgccctgc
    21121 aggcgcgtcg aggccgacga gagcgttatc tgctcgccgc cttcctcctt ggcgccctcg
    21181 gccaccatga tgatgctgaa ggggctgccg cccttttcgc ggaactgtat cttctccacc
    21241 accttgtcga tgttaaaggg gatctcgggg atgaggatga tgtgcgcccc gccggagatc
    21301 cccgactcca tggatatcca gccggcattg cggcccatca cctccaggat catcacccgc
    21361 tgatggcttt tcccggtggt ctggaggcgg tccagagcgt cacaggcaac ctgcaccgcg
    21421 gtctggtagc cgaaggtata gtccgtcttg tcgaggtcgt tgtcgatcgt cttggggatg
    21481 ccgatcgtgg gcacgccctt cttgtggagc ttgtagccga tctcgagcgt tccctcgccg
    21541 cccacgacga acaggcagtc caggcccagc ttccggaaat tagcgaccgt ctcgtccgag
    21601 cggtcggtga cgctgatgga tccgtccgcc ttgagctccc ggtattcgaa cgggttgccc
    21661 ttgttggtgg tgccgaggat ggtgccgccc agcgtaagga tgtcccgcgt gcttttcaca
    21721 gtgagctcgg ttgtccggtt gaacaccagc ccgtcgaagc cgccctcgat ccccacgacc
    21781 gcggcattgt gcccgatcgc cgccgcgcgc gtgaccgcgc ggatcaccgc attgagcccc
    21841 gagcagtcgc cgccgccggt tagaatgcct acttttttca gtgtcatggg gttcctccgg
    21901 tttcttttga tgcgctcgct acctcatccc ccaacccctt ctcccatagg agaagggggg
    21961 attggcgaga gatatcttct ggcacacccc tctcctatgg gagaggggaa gggggtgagg
    22021 tggtcgttcc tctaccgtct tgtttgatta tatcgtaatg ctaaactgaa gatatcatat
    22081 ccatgtacct ctgtccacaa gaattctatc gcaatcgatt ctcccgtacg gtgcgtgtgc
    22141 gcaataaaca tattgacgct gccgaaatcc gtgagtacat tgttgccgcc gggtgtctct
    22201 cggcgccgtc gtatatacca agggacggtg ggaggtaacg atgaagtcgg caaaagcaat
    22261 cgcggtaatc ctattcgcac tcggggtcac cgctgcgttc gcctggaacg tcggggaccg
    22321 catcctcggg cagtggacgg acgggatgtg gtatcccgcc aaggtcgccg gggtgcagga
    22381 tggaaaattc gcggtcacct tcgacgatgg ggacgtgggc gtgctcgcct ccgcgcagat
    22441 caagcggatc gattggcgcg tggggacgaa ggtgcagtgc aatttccgca atgcggggct
    22501 ctactatccg gggaccatca ctcagatgcg cggcgaggtg atccacatca gctacgacga
    22561 tggcgatcag gagggcgcca ccatcagccg ctgccgtgcg cggtaacgcg ttacgtccga
    22621 aatggcttga cggttcggcg gtgggtccta ggatgcgtca gtattgattt ttctttcgcg
    22681 gagatagtat gacggctctt gttggatatc ggttcccgct ctgagggacg aagcgatacg
    22741 acgaagctga aaaccccatc ctttgttgtc ccgccccgtc gttcgagaat agttctctct
    22801 ttcagagcag gaacggacct tcgtgtgttc gtatctactt tatttacagg agagctgtca
    22861 tgaacgacga atatagattt cgcaccgcgc agtcgccgga cgacgcggaa aaactcaaaa
    22921 accacttcga cggcatattc cttcccgaaa aggtcggaga ggtggccgtc gcgctgtatc
    22981 atcaccgtcc cggcatgggg ccggagaact ggttcatcat cgaggaggcg accaccggcg
    23041 cgatcgcgtc ggcctgtacg ctgattccgt gggtatggga gttcgagggc gtacggctcg
    23101 acgtggccga gatggggctc gtgggaacgg cggccccgca tcgcgggcgg gggttgatga
    23161 acgaactcgc gcgccgcttc gcggaggaga tgaaccgccg gggatgcgac ctgggcggga
    23221 tcgaggggat cccgggcttt tacgcccgct atggctatca ctacgcggtc ccgttggaaa
    23281 accacatcaa cctgcccctg catacctgtt ccggcatgga ggccggcgcg tatgcgctgc
    23341 gcacggcgac ccgcgacgat atcccgtttc tcatggcgca ggacgacgag taccggcgcg
    23401 cctattccct ctcgtcggtg cgcgacgcgg cgtcgtggga ctatattttt ggcccgagcg
    23461 tcgacaccga atgcgcgcgg gacatcagga tcatcgagcg cgacggggcg ccgctcttct
    23521 cctttgcgat gatggcctac gggttcggcg cgggcctcat cgtctgcgag gtaagcgagg
    23581 ggatcacgtg cgaggcgttg tgcgcgatgc ttcgctttct cggggaggag gccgcgcggc
    23641 gcggaaagcc gtatgtccgc ttcaatctgc atgtcgattc gtccccggcg cggatgctga
    23701 tcgcgctcgg cgcggcggtg aatccgggct gggcgtggca ggtgcgggtc ccgcatcccg
    23761 agcgctttct cgcgaagatc gcgcccgcgc tcgatcgccg gctcgccgga tcaccattcc
    23821 gggagagcac gatggcgttc ggcgtgaacc tttattgcgc gcggtaccgt ctggcgctcg
    23881 cgaacgggcg gataaagtcg gtcgagcgcg atgcgggcga atgcgcgcat gcgatcaatg
    23941 tgagcgcgga cctcttcccc gcgctcgcgc tgggagcgca cggctggcgc gaactcaacc
    24001 gggtgcggcc cgaggtgtgc gcctcgagcg acatggccgg gctgctggcc gatgcgctct
    24061 ttccgccgag acggtcgtgg atgcggcttg cgtattgacg gtgcggggcg gttccggatt
    24121 tttcgcttgt cgggatcgcc ccggccggct gttttgtcgt tcgggcgcgt cggcgcatgc
    24181 cggggcgtcg cagttttgtt ggcgtgatat ccacaaccat gcggaggtgc atcatggcga
    24241 aatcgaccga tgcgcgaaag gacgcgaaga agaagccgga gaaaacgatg aaggagaaga
    24301 agcaggccaa gcagcagaaa aagaacgagc gcgatcgcga gaacaagtcg ctgtaacgcg
    24361 ccgcgcattc cgcccgcatg tgtggcgcat cgtatccgcc ttgacagaat gggccgtgtt
    24421 cccatcgttg acaacgtttt tcatgcggat ggcggatgtg cgcacactat gtacagggcg
    24481 gtatgatcat gcttgaggct tccgatgtaa cactctcatt cgggaagcgg gttctcttca
    24541 agggggtgaa catcaggttc acctccggga actgctacgg gcttattggc gcgaacggtt
    24601 cggggaagtc gacgtttttg aaggtccttt cgggcgacat tgagcccaac gagggggcca
    24661 tcatccgaaa tcccaaggag cggctcgcca tgctccagca ggaccagttc gccttcgacg
    24721 agtacccggt cctggacacc gtgatcatgg gtcaccgcca gctctacgag gttctgcgcg
    24781 agcgggaggt cgtatacacg aaggaagact tcaccgaggc cgacggccac cgcgccgccg
    24841 agctcgagga gcttctcggc tcgatgaacg ggtacgaggc cgaggccgac gccgcgacgc
    24901 tgttgtcggg cctgggcatc gatgatgcgc ttcacgggaa gctcatgaag gagctcgagg
    24961 gtggacaaaa ggtgcgcgtg ctcctcgccc aggcgctctt cggcaatccc gacatcctgc
    25021 tcctggacga gcccaccaac aacctcgacc tggaatcggt cacctggctc gagaatttcc
    25081 tctataactt caacaatacg gtcatcgtgg tctcgcacga ccggcacttc atgaacaagg
    25141 tgtgcacgca catcgccgat atcgacttct cgaagataca gatctacgcc ggcaattacg
    25201 acttctgggc gcaggccagc cagctcgccg tgaagcagaa acgggaagag aacaaaaaga
    25261 aggaagacaa gatcaaggag ctgcaggcct tcatcgagcg tttcagttcc aacgcgtcga
    25321 aggcgcgcca ggcgacctcg cgcaagaagc tcgtcgagaa gctcaccatt gacgagattc
    25381 ccaccacctc gcgccgcttt ccctatgtcg cattcaagcc ggagcgcgag tgcgggaagg
    25441 tgatcctgaa cgtggagaac ctctcgatga cgatcgacgg cgatcccatc atcagggatt
    25501 tcaccctcac ggtgaatcgc aacgacaaga tcgccttcgt gggccagaac aacctcgtga
    25561 agaccgtgtt cttcgagatg atcaccggcg cggtaaagcc cgacggcgga gagatcaagt
    25621 ggggcgagac gatcacgacg gcgtatttcc cgaaagaaaa cgcctcgttc ttcgagagcg
    25681 acgtgaagct cgtggattgg ctccggcagt tttcggacga gaaggacgag accttcgtgc
    25741 gcggctttct cggccggatg ctcttctcgg gcgaggagtc gctcaagaag gcgagcgtgc
    25801 tctccggcgg cgagcgggtg cgctgcatgc tcgcgcggat gatgctctcc agcgcgaacg
    25861 tcctcatcct ggacgagccg accaaccatc tggacctcga ggccatcacc gcgctcaaca
    25921 acggcctcat cgccttcccc gaggtggtgc tcttcgcctc gcacgatcac cagttcatcg
    25981 attcgatcgc gaaccgcatc gtcgagatcg cccccggcgg cgtgatcgac cggtacatga
    26041 ccttcgacga gtacctcgag gacgagggcg tgcgcgcact gcgcgacgag ctgtatcacg
    26101 ggcaccagcg cctggcgctg tgaggggcta gtcgcccgca gcggcagtcg tatccgttcg
    26161 cgtaaacagc accggcgcga tcgcgatcca caggagcaac aacacccaga gttcgccctg
    26221 ccagatgcgg tgcatgtcga tgaaaacctc caccggcttc ttctggagga aaacaatcat
    26281 ggaaatctcg aagacctcgg tcatcgcgac ccacaggagc ccgatgccga gcgcgcggcc
    26341 cttcgacgcg agcggccagc gccggcacgc ccgccatatg atgaggccga agatggccat
    26401 gcccggaaag accgagagct ggtgggagag gttcccgggc aggagcttcc cgtacgtgaa
    26461 atccctgatg ccggcgttga cgatggcgcc tgccaggagc gcgaaccaga gaagaatgta
    26521 tcgtgtcatg gcgttccgtt ttagataccc cgtcatttct cgaacgaacg agcggtggcg
    26581 ccggtatact cgcaccgggc cgcgaatacg gcaagcgaaa tactgccgat ggcgtcggta
    26641 tgtcttgaca ttcgctcccc tcgccgctac aacggatacc aatcacaatt cgatgattcc
    26701 ggaggcccgc atgaacctgc cgcccgtata cgccgaactc tcgaagaagc tcatgatgga
    26761 gcactcgaag ctcctgccgg aactctggcg catggtgtgc gacgaggacg aggcgaatgc
    26821 ggtgaacgcg cttcccggca ccgccgagga aatcgcgcgg gcgctcggca ggcccgtggg
    26881 cgacatggag atgctcctca agcggctcta tcatcgcggg gcggtgttcg attccgtgaa
    26941 agacgggacg ctgtactacc gcatgccccg gcaggtcgtc caattccacg atgcgacgct
    27001 cctctgggac gaggcgccgc gcgagctcat cgatctctgg gtgcgcttca tggacgagga
    27061 gtatccgcag cttctggagc tggtcaccac ggtgaagttt ccctccttta tgcgggtgat
    27121 ccccatcggc gagacggtcg ctccggcgtc gagcgtgctc tcctccgagg acgccgtggc
    27181 gatggttcgc gccgcgaaga cgctcgcggt gacccgatgc gtgtgccgcc tgtcggtgaa
    27241 aaattgcgac aagccggtgg agatgtgcct gcagctcaac cgcggcgccg agtacaccct
    27301 gaagcgcggc accggtcgcg cggtgagcgt cgacgaggcg ctggcgatac tcaagaaggc
    27361 cgaggaggcg gggctggtgc acatgaccga gaaccgcgcc ggcatgggca acgccatctg
    27421 caactgctgc gactgttgct gcgagatgct gcgctacgcg aagaacgcat cgacgaaggg
    27481 cgtgctcgcg cccagccggt atctggccgc ggtggatgcc gccgtgtgca ccgcctgcgg
    27541 gagctgcgcg gacatctgcc ccatgggcgc cattgaggtg gcggccgata gcgccgcggt
    27601 ggcggcggat gcgtgcatcg ggtgcggtct gtgcgccacg gtatgcgccc ccggcgccat
    27661 catgctacag ggcgttcgtc cggaggattt cattcccgcc cgatgagcgt cgctagcccg
    27721 atgcgacggg cggggaaatg aaaaatattg tttgctattt ctctcgggcg tggtacggtc
    27781 ggaccattcc cataaggccg tttggtccca gtaaatgaaa gtagacacgc caaagctcca
    27841 gaatctcctt tcgaaatacc ccaccgagtt tacctgcaac tatttttccg atgtggtcgt
    27901 cgtgggcaaa aagctcaagg cgggagaggc ggtgctcctg ttcgatgtga acaacatggt
    27961 caaatcgggc ataaaggacg tcgaggtggt ctacgacgtc accatgtacg agtatctctg
    28021 ccgcgaatac ccgtcggagt accgccggcc ggtgcggtgg ttgtcgtatt tcgagatcga
    28081 caaggccctc gaggagatcg agaaggtgaa cgcgcagacc aagcgcaagc gcttcctggt
    28141 gaacatcggc gacgtgtacg aaaaggaggc caagggacag aagagcggcg cgatcctgaa
    28201 gcacaacgaa aagctcgact accagaagtg gaagaccgtt aaaatgcaca tcaacagcga
    28261 gcagaagttc ttctgccgca actccgagca tgggatcatc ctgtttatga gcatgaagtc
    28321 ggagggcggc gccgactacg tggacaagtt cagtaaacag gcggacctca tcgccgccat
    28381 ggtctcgcgc cgcaccgaca agaacgagat cgcgcccgat ttcgtcccca ccgaggacgt
    28441 gcatcccgtg accgtgccgg ggcagctcct tgaagagtat atccgcacca atgcgcggct
    28501 catcatcatc ggcgagaaca tcaccggccc cttcaaggag gcgctgctgc aggtgaagag
    28561 ctacgacccc ttcgtgcgga tgatggtggc cccgccctac gatcccaaga ccgttgatca
    28621 ctttcttcag caggtgaaga tcgtatacaa tgcggaccgc tggaagaagt agccgcacca
    28681 gcttgtttgg cgcgctctgg agccgggagc ggcgccatgc gcatggtggc gctccgccat
    28741 tgaccgcccg aaacgccgga catcctttgg aggcatcctc ccatgaaaat acgaccgccg
    28801 catgcacttg tcctcgcagc gttgttcgcg atgacgatgg cgaccgcatg tgaccgatcc
    28861 gattcgcgct gccgctcgca gatcaagaat ctcctggcgg aatgcgagtc gtttattgcc
    28921 gatcgcgagg aggagcggtg gaagcaggtc tcccatgccg tgggtaatgt catcatccgc
    28981 gaaatctttc tgggcaagcg gaatgagcac atgcacatcg cgctcacgat cgatcatttc
    29041 atgagcgcgc gtgagatcgc cgcgatcggc gtccagataa aggagaaggg cgatttccag
    29101 cccttcatcg ctatctggcg cgatcagggc cttgtgcgaa aaaccggaag agaggccgtt
    29161 gttcccgaac gcatcgcgaa ggtgtcgacc gcggccgaca ccgtcatcga cctgaaggga
    29221 tctgggggcg ggacgagcat acgggcaacc gtgttctatt cgccggaggc cgtcatgtcg
    29281 tggatgcaaa accagaagaa gatattcggg gccgagtgag agggggcgct gccatggatg
    29341 gatgccgtat gtcgcgaatc acgaattgcc ttcgtgcggt gatcgccgtg ctgccgttcg
    29401 ccttcgctgc gatcgtagcg tgcgacctgg gcgagacccg gtcgggatcg atgggcaggg
    29461 agatatcctc cgagcaggac gccgcaatgg ttctctcgga gttaggcatt gcccctggac
    29521 cgacgctcgc gagcatccag cgccacccga agagtgccgg gaccttcggg cgcgaggggc
    29581 tgcgcatcgc ggccgtctat cgcgtgagcc gggagcgggc cgacgccatc ctcggaacgc
    29641 gattggccgc atggcgacgg gggccgttgc ccgccatagc gaggcggttc gaacaggggc
    29701 cgaaggagct cgccgcgtat tcgcccgggg ccttcacgcg atgtatcgca tggttcgccg
    29761 aaacgggaaa gccggtgccc tgcgatgatc ccgccttcgc ggcggcgttc ggcaagaacc
    29821 cgatggcctg ccgccactac cgggccgccg tgctcgatcg gaacgcggga acgctgacgg
    29881 tcgtctacaa aaactactat tagcgcgccg ggcggttcca tggagcggac tgaaacgagg
    29941 attggtgtca tcaacggcat cctgagcgcg ctgcatctct attcgctcgc gcaggcccag
    30001 ggtctcgtca tggcgacggt gatgacgctc ggcgcgccgg catcgcagga cagcgcgctg
    30061 cgcacgctca tcgggtatgc cgtgctcgcc catccggtcg taacgctcgt ttccctcgta
    30121 tcgacgtggg tcctcttctg gaagggccgc tatctcctct cgttgctgtg cgcgcttctg
    30181 cctgttgcga acgcgattgt cgccggcgcg gcgatactgc tctcggttct actcggaagg
    30241 cctatcttcg gctgaggtgg cggtatcgat cacgccaccg gcgcgtctcc attgaagtga
    30301 cttgttgcgg gaatactcga aatattggcg gcggagttcg tggctgaact cgtcaatgcg
    30361 gttccagggc atgaagtggt gttgttgttc aagcgtatca tgtggctttt ttccagtaca
    30421 gtcttcggaa agagtcgggg tatttcattt gtggaagtga atagaaagct gttttgtgtt
    30481 ttgctaatcc tgctggaacg gattatttct tgcgaagcga aatttaatgg ttgtagtggc
    30541 gaaatcagaa atgactgtgg tttatgttcc tgcactcctt cgggaaggag cgattatttt
    30601 tggttaatgt gtggaaccat tggaacgcag gttctgtgtg tgatcggaaa ttacttctgc
    30661 taaccgatgg gatactggta actgacaacg aaatgttcag ggagaatctc aatgaagaag
    30721 tatttttttg tagcggtgtc attggtttgc atggtttgta tgacctctgt ggcggcgttc
    30781 gcggatgaga tcgatattag caagatcgcc ttagagcgtt tgaggaagac cgctgcggca
    30841 acaaacgccg cgggtcaaaa gctggtgggc gtgaagtttt taagtacgct cgaaaccacc
    30901 gggatcaaat tcaagctccc ttcggcggta ccggagcagc cgttcgtgtt gatgactcaa
    30961 acgctttcca ataacctcgc gtcggaagca agactcaaat gcggcgtgga ggaatccagc
    31021 atggactccg agacgatcgg cacgtcgaaa accttgggcg gggaaacaga ggtttcgctg
    31081 agctataaga gcccggtcgg tttgaccgtc gccggtaaac agtctttcaa ttattccacg
    31141 acgaagaaca tggagaagac caccagcaaa acgaggacct ggcgcgccga aggggacttt
    31201 ccggtaggac ccaaggagcg gatcgatgtg cagtttgtgg tttccgagaa gcggttggac
    31261 aatattccat ggtcc
//
LOCUS       DAORHF010000012        30968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_863_length_30968_cov_6.258694, whole genome shotgun sequence.
ACCESSION   DAORHF010000012 DAORHF010000000
VERSION     DAORHF010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 30968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30968
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_30968_cov_6.258694"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PKJ16_02120"
     CDS             <1..396
                     /locus_tag="PKJ16_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019594292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNV45807.1"
                     /translation="PTFIETKAHNAVESYALSPDGTIATTFTFRDGSFDGPAKRYTPR
                     GFVVNRKTNAEWGMQFIWPIKGEFLIVYLRDDYGVTIIGRSKRDYVWLMARTPSIPEE
                     EYRALVKFIGDRGYDVGRLYRVPQKWDAR"
     gene            complement(421..699)
                     /locus_tag="PKJ16_02125"
     CDS             complement(421..699)
                     /locus_tag="PKJ16_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45808.1"
                     /translation="MDLVIDFVRRNIWFILFFAWGLPLGYYRSRFRKIVYQTDSWIIN
                     IKPVFVKELRALFGTMYPDNPDYIRFRNFYRLYLAIYTALFAAWKIFS"
     gene            complement(709..1407)
                     /locus_tag="PKJ16_02130"
     CDS             complement(709..1407)
                     /locus_tag="PKJ16_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45809.1"
                     /translation="MALLLLIGTVAGEVRAEALFNADLYLAGTNRQQLIFRQKNSIAK
                     KDGATILTHTYTHVDGRLAAREVVTRKDGAIQRYEVTMPDTGCGCLLETAGNRIRFSY
                     TKGGEPKTGEVDVVPDIVTGPTLTAFVHRRWASLLRGETAYFHLPAMSLQRLARFQIT
                     RIAGSPRNRPGVEVFQMKIANVFLRIAVDPVELVYDSASRRLLEIHGKSLLTRRVGGR
                     LENPVVDIVYRYDR"
     gene            1560..2339
                     /locus_tag="PKJ16_02135"
     CDS             1560..2339
                     /locus_tag="PKJ16_02135"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HNV45810.1"
                     /translation="MGEILNSYSIAAIFVFGYMTVLFLLAVFLKDNSIADVAWGPGFI
                     IAAAAVMIVQETFGVRNFLLQALVTIWGLRLGVRIFLRNHGRGEDWRYKKWREEWGSL
                     FYLRSYLQVFALQGAILLVNAAPLVIANSAPGDILVWTDYAGLAVWCTGFFFESVGDY
                     QLDRFIKDPANKGTIMDRGLWRYTRHPNYFGEVTMWWGIFIIGLGAPFGYLGIVGPVI
                     ITLMIVFVSGIPMTERAMDHDPAFQAYKKRTSAFIPLPPRG"
     gene            2493..4949
                     /locus_tag="PKJ16_02140"
     CDS             2493..4949
                     /locus_tag="PKJ16_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV45811.1"
                     /translation="MFVQDHHKTAFIHRDRPISYRELLSRITSFAGAFRIKTGDRVAL
                     CAENRPEWIYAFYAIWQNGGIPVPLDPTAAPADLAVVLRDCRPSLALCSGECAGRVKQ
                     ALRTRGMPKTTVRTLESLPDRADGPATPVTGGAPDDTAIIMYTSGTTGAPKGVMLSFA
                     NLAASIRGVASLGMLTADDRMLGLLPFYHIFPLQGTVIAPMQIGATVVMVASLSPEDI
                     LATLQKHRVTMFLGVPKLYELFHAGIVRKIRANPVAYGLFRVMRRVNSVRAGRVVFRK
                     VHAAFGGQVHSFLTGGAPMDATVARELWSLGFRLVEGYGLTETAPLVAFNPIDRIKLG
                     SVGKPMAGTEVKIDNGEVLVRGENVMKGYYRNPTATAGKIRDGWFHTGDLERFDDDGY
                     LYLEGRVDEMIVLPSGKNINPDEIERAILSSSPYVAEIGVFAHRGHLTALVRPDFAAL
                     ERDGIGTIAETIKWKVIDAYNARVPGHKKVLGVVITRDELPRTRLGKLKRFRLPEIAA
                     AARPSSGRAAGPTTPEYGILKRFLSSHAGMEALPDHHLELDLGLDSLDRVELLVFIES
                     AFGVSLDERDLAQFTTVARLAELVARRKTRAKEAAVDWRGILQASEPLSVRGGMFLMR
                     IVRGVARPFLRIVFRLRYRGLEHLPQAPFILSPNHQSFIDVLLLAVALPDRVLRNTFF
                     IARESLARRALVRALTRRTNIIFVNFERDVKTALQKAASILKAGRNLVIFPEGHRTRD
                     GRIAPFKKTFAILGAELQVPVVPVAIRGAYEALPAAGRLSRPRSIELTFCAPVRIMQG
                     NYAARAESVRSIIRGVVERA"
     gene            4980..5507
                     /locus_tag="PKJ16_02145"
     CDS             4980..5507
                     /locus_tag="PKJ16_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV45812.1"
                     /translation="MNAHPSSCEFRLTLGDGRAVFVRPIAPNDAPLLVDLFNRLSPQS
                     IYLRFLSRLSGLPADMLHHFTHVDYDREFALAAVVAETGGDAVIAVSRYSHDRDTGIT
                     DLAVAVRDDWQHLGLGREMLARIVAIGREHGYHRYGSMMDPDNTAIRALLHELGYRVK
                     YTPRDGYFDVEIRFD"
     gene            5595..5774
                     /locus_tag="PKJ16_02150"
     CDS             5595..5774
                     /locus_tag="PKJ16_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45813.1"
                     /translation="MEKAELRKDEFVALFTHFGLEPDWKFLSENFATIPDYEWKGFLN
                     SIHGILMRRQNRREG"
     gene            complement(5785..6801)
                     /locus_tag="PKJ16_02155"
     CDS             complement(5785..6801)
                     /locus_tag="PKJ16_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV45814.1"
                     /translation="MAPRTEADSIMPINTHAGRITDHVHLIDTLQFGKPAVTAAFLVW
                     DGRTGLVMDTGTSDNAPAVADAIARLGVPLGSVAGITGTHYHFDHMGGASALARIMRA
                     HNPSFSIFAPADMKARLQDAEEHLAGARTTFGDFVGTMDPAPEECFTIVAKDAALPID
                     FDGGISVTLMATPGHCPDHCSPAVRSDGRTEFVFAGEACGTLFHDSELVSSATSMPPN
                     FSFERYMAGVETIRALDPGAVGLCHFGAVTGARDVTEYIARHRDYIRTFRELIITAHA
                     ESPELRHILRATRDYFARSFDPAREIPEAFIGNLQLALTYGMLVDLGLREPKYEKRLP
                     EADA"
     gene            6912..7607
                     /locus_tag="PKJ16_02160"
     CDS             6912..7607
                     /locus_tag="PKJ16_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HNV45815.1"
                     /translation="MKHTRKAAPRPARVGAFFDLEKTIADHAVIWEFVMELYRRREMG
                     LSVVVRTMWIYFKYSLGVVKNFETVMRDASRRAFQGRNAARYIEIFRELFDERLKHRI
                     YPGARDTIAELKKRGVRTYIISTTFQSLVEFYANHLGIDEFYATSLEIVDGIHTGAIN
                     GPIYYYQHKASLMMEIAARDNIDLARSFAFGDNLNDRVMLECVGHPHAVNPDKKLRAV
                     AERGGWTILSWGI"
     gene            7611..9698
                     /locus_tag="PKJ16_02165"
     CDS             7611..9698
                     /locus_tag="PKJ16_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV45816.1"
                     /translation="MDKFFYPESMAVIGISIGRITLGNIILMNNVNLGYRGRLYGVGK
                     EAGELQGVPVFTSVDELPETPDVAIIITPAKTVPGFLRDCARKGVRHVVIESGGFSEF
                     STAHSTLEDEVRAIIAEHGIRLVGPNCVGAANKEINMMMAFGFFKNEGPPSGVAAITQ
                     SGGIGNTLIRIVSDNNIHFGKFISIGNKLDLDESDFIEYFLRDEKTDMILMYLEGFKR
                     GRFFFELAMRSDKPFVVLKSNRSELSAKIAQSHTTAISASDDVVDAAFAQAAVTRVEG
                     EDELTVAAKAFQLPVMRGRRVAVLSRSGGHAVITADACAKYEFDMVQFPASYIENLKT
                     IYTTRVIAHQNPLDLGEIFDYTIFIKILEETLKLDGIDGIIFNHLFQSGYESESSRTF
                     LTAVKDLVAQYKKPVAVSIMTNAEELLDINKNHPFPVFASPLQAAAALSMSLAYHERK
                     TARDARGTAPSFPVDAAAIEAIRVRAVGEKRIPLTDEAHAVCAAGGIASPALRRIADE
                     NGARGIDLRFPVVLKLLSRDASHKSDVGGVRLGIESADALAAAVSEMRSAVSGAPTPV
                     AIDGFLAQEMAPRGEEFFVGARRDPVFGPIVVAGYGGIFIELFRDRAIRVAPLTASEA
                     RDMVRSLKTYPLITGLRGRAALDEDAFVELICRVSNLVTRVESIAEIDLNPVILHERG
                     KGLSVVDARVFFA"
     gene            9715..10734
                     /gene="mnmH"
                     /locus_tag="PKJ16_02170"
     CDS             9715..10734
                     /gene="mnmH"
                     /locus_tag="PKJ16_02170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HNV45817.1"
                     /translation="MKEITYAEARALASPVFIDVRAPVEFVQDHIPGAINIPLFDDLE
                     RAEVGTIYRRIGSDEAILRGSEIAGGKLGEIVARIRRLPSDNLVINCFRGGMRSSTLA
                     AILDSLAIKNIYKLEGGYKEYRRHVGAALAAHRFSQPVYVVQGLTGTGKTAVVRALEH
                     AIDLEELAGHRSSVFGAMGMVPHSQKRFESLLLAKLESMRDAPYIVFEGEARKIGDLF
                     VPDCVVRAIAAAPTIMLVADIERRIDIILEEYEAHIDPAAVSAIVRSLEGRIGKKTAA
                     ELLDALDGGRYREFVRVLLERYYDARYAHSFAGCDIIAEVENADTAAACRAIERIIAE
                     RPGRM"
     gene            complement(10970..15835)
                     /locus_tag="PKJ16_02175"
     CDS             complement(10970..15835)
                     /locus_tag="PKJ16_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HNV45818.1"
                     /translation="MDAAQSTQSSLSRFTLREERQISDELVKIELRQAFAENRCFDFF
                     PSHDLVKLINAGDGPETLADRYIDIKNAAFGRVSGDDRTILARLGSDARLSSRIVAAV
                     DFVHRAIAADNNLSLLARRFVLVKERHGAPTLHHVSNETTVLAHVGQGPHWTEIPTIY
                     LGLNVFDALAAEEKRGGRDLFDAFRLVLKIEERAIQTGYAHTEVFSPEAARALQSLLD
                     EVIRISAVTEVEPEAAVPERALKGFTERNRQSHLRLLNARVKGDELNFHWDKNLKAIA
                     SLERLARRYKKTSDAASLREVMRLLVAASGHDIHEVRNKANMLLERVLAPKEFDAPLA
                     TEFVNVHVGETHRFAFSLPDSRGGYFVRIYRNAAAGRIPTQDEIAFDDIELTPDESGA
                     VFIAERRFEEYGHYDYLVYRRKVKNAEWLTLPGASGRINVLPNVAGEIILEIFPDIHG
                     HTGIYWRDPSGHPGLVYNENGEVIRLGRFADIAAHLEDIKERYRITAIYLLGAQRRGT
                     NRSDWAPGATSPSPFSPMSLTEIEPYLGGPEEFRALVARAHALDVKVIVDVVPHLNRM
                     SDVLPDDCAVRCYDGGGALVFRASTDGRYGSWDDGRLFNYRKLAVWDWLADSIVTLIE
                     EYDIDGIRFDSAHAVPIMMKRNNYLFTYDEARSDEDMVEGAIIVNDREDEHFVTTGFY
                     DSACRDILAVPLHYYYMLAVERAVRARGKRFFINIAECYWGHERFLTRLGLIPYNSAL
                     FKICENIVHGKTDVREIYHIYDNYFPKSLPRGTELLGILGNHDERRALNTFGHRGLRA
                     AVALTMFMSNIVMDYEGSAEGEGWKVFLDNIYVNWNQFEYAAHRSVEQFYRDWYDFHR
                     TATGKGHLVWANNTMVAAAMKFTATDTWIGVFNFADSNQNASIQFDNPVLPIADDALF
                     KVIDPLYSPITNTYSYFTGRELKTARVNTVVSFTERVKLLRLETVDQLERHYQDFLRD
                     SFFRLCAIGVPAHFTASFAFREITAALHDFDGLANFIISHLLPLFWADHRPTLELGLK
                     RSLYHAFKNGLFDGDTLRGYMNRLAGHEDERLRELGAALLHHSAAGSLVFMSAEAEPF
                     SKSGGLANVVYELPRELVNLEEEVYVITGYYRSGDDKSQRKMREAATKYGVRYAGMNV
                     RFKIMDHDYDVGVHRGEVDGITYFLLDHHEFFDGLYWGYTAEEKLRRRIAFARACAEV
                     ICCFNLKPLFTFTNDAYAGIFDGIVRSDHVYNDNPNFRRTTFLHIIHNGGWQYFDAYH
                     RFERGFDLFSLFNLPSWKAGDFSDPVFGDRLNCMAAGIRFADRTITVSPSYARQIEYA
                     CDGLERILHNVIGISNAIGRDFRERIDRNFAASGFQEEHYPAFKEHLRTDAKLAATVE
                     RDFPEILRGLDAVRAIPDALRRGSTVRTLNKMLLQLQRGFTVDPRAVLFTMIHRITEQ
                     KGFQLLLDASEGIFRDLGYQAIIGGAVSSGDQRGEDIAHGLYLLGDYYRDRVSVSFGF
                     QDISIPLLASDIFGMPSMNEPGGISQLEGFACGCLVVARATGGLRDTVFPVRVRDDAI
                     EGNGFLFSDFTPWAFYDAMERAHRFFTANDHLTLQKARVNAERSIYFWDTPARKYIEA
                     IYGIKEIIRVI"
     gene            complement(15841..16560)
                     /locus_tag="PKJ16_02180"
     CDS             complement(15841..16560)
                     /locus_tag="PKJ16_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45819.1"
                     /translation="MARRCLCLWFVILFAGCAARSQFALTGAARTPLPDTAAVAVVPY
                     GDTGAYDTIGTVEVRSSSADKRLAEAKRVARANGGDVIMPRGGEEKHPSIQTFLVLKS
                     RPAQPLDTGKDIAIKDRKGSDVDGGDFGKLPRATYKMLVHDYRDLKDEKFRGSLYPVR
                     FVKRPPRALRRHGGEDDAVLLMSTKSGRSRLYVIVPEEKVDDIKESIKERARINFIYT
                     PVSLHTERGKRYPVLRLIDTK"
     gene            16789..21660
                     /locus_tag="PKJ16_02185"
     CDS             16789..21660
                     /locus_tag="PKJ16_02185"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV45820.1"
                     /translation="MDPTTDRRRHRPSYIGRAFFSLLLLWIVLEYRFTWLFVPAIGFI
                     AYAFVWMLLVEFGRRVPVDHIAVKFVRTVIDIAFITFFVYLTGTVQTFLLIIYPVWVA
                     GDSMNVRRVYGVFTTLVASLAYIALVLLEYFSVVPDINIITETPAHIGTLQMGVFIVL
                     MVTINFGVHALVYGLYKRLADKNEELSRANAAIRQSEARYRTILEHIEEGYFEVDLTG
                     RITFFNAALCRISGYTAEQVDGRSYASFMDPIFVPGIFKIFHRVYETEQPSELFDWYF
                     VHQDGTRRIAEGTASLIVDAEGAKIGFRGLLRDITTRRAAEEALRASEEKYRNILEGI
                     KEGYYEVDLAGAVVFCNDSFCAIFNAPRERVVGKNYASLMSPESAGRVFGAFNDVFRE
                     RDHRELVDWEITLHTGERKFLVASIDIIADGSGRIAGFRGMIRDNTQRMLAEEALRTS
                     EEKYRDILEGIREGIYEVDLTGSFTFFNGAICTMWGRPRGRMVGLNYRNYLDQDAARR
                     VREVFTETYRTGKRNYHSDWIVTGDGEQRYIEISVEPVVGQEGTVLGFRGLARDMTER
                     KRAEEALRESEEKYRHFVENATDGIFRNDAKGNFLFVNPSGLRLLGYDDTEITSINYR
                     SLVLPEYRDFVTAAYRSQISNNVAESYLEFPVNRKDGTVVWLGQRATLERSARGTCEF
                     HGISRDITERKRAEDDLRRSEERYRTILESINEGYFELDIRGSFTFFNKALAEIWGRP
                     RNVIMGMNYREYLSDEDRERAYAVYNEVYRTGNRGAFLDYEVIRPDGSTRYVDSSFDL
                     MHTPDGTPVGFRGMVRDITERRQAAEALRRSEEKYRGILETIKEAYFEVDLAGSFTFF
                     NGTLCELSGYSREELIGLNYREYMDAGTVARVFETFNKVFRNRTAEPLFDWDIVRKDG
                     GVRSVESSVDLLYDAAGNPAGFKGIIRDITEKKRAEEALRELDRQKSDFFANISHEIR
                     TPLTLILAPIESALQGDYGETMDRGMLVNVQRNAIRLLKLINNLLDFSKIEAGRMAMK
                     VREADIVAFIRRYVDTVRPAAEAKGVALDFESGAGSLPLSFDAEKIDKVFMNLFSNAL
                     KFTDHGGRIAVRITADERNCYIEFADSGIGIPAEKLDVVFDRFGQAAVGANRRYEGTG
                     IGLALAKEFVEMHDGIITLVSRCIDEHPDDHGSVFTVTLPRGMEHFEGRRGVEFVTAD
                     ELDESVADHRFAGMREMLDLREDLSGAAPEPGPAAGALADNRRTVLVVDDNADMRAFL
                     RTLLSRSYAVELAEHGEEGLARARSLRPDLIVTDVMMPVMNGYEMTRRIKADAELMHT
                     PVIMLTARAEVTHRIEGLEFGADDYLTKPFNSKELLTRAANLITMKAQERELGIAYES
                     LNRAYLQMKEDLLLAKTIQANILPANDSTACGLSLHVEYLPLIEVGGDVYDICEIAPG
                     VVRVFLADAIGHGVVASLITMLIKSEYDMLKASGASPFGVFSALNDVFFMTYRSLKVF
                     LTGVIIDIDTARGVITYSSAGHPVQYLLRGGETVPLAKTGRAVGIWGGINCRDVEVPY
                     RAGDRLLLFTDGIYEEFNSENEEFGEDRVRGIIEAHAGEPIRCVIDALLEGMTGFVGD
                     VGLNDDITVLGVE"
     gene            complement(21721..21966)
                     /locus_tag="PKJ16_02190"
     CDS             complement(21721..21966)
                     /locus_tag="PKJ16_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV45821.1"
                     /translation="MKKQNREEEMIVYGTVKVSDKGQIALPVELRKDLDIARGDQLLI
                     VRKKNSGTFAFMTMKEVMKSIQDLWVDDFVPEEFRSK"
     gene            complement(22098..22535)
                     /locus_tag="PKJ16_02195"
     CDS             complement(22098..22535)
                     /locus_tag="PKJ16_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45822.1"
                     /translation="MFPKQRIHRRDGEQQQPDNEYHGRTDGPFGGKPNEKRLQDENPE
                     NPEKHNEDQQRVADFQFEHFPGVNDGEIRQHDPEKQQAGVHHIEIRSDDGQALTGQSR
                     EVLVPLEHPGKENMRYGAEQVKNAPDTKQNGIHIDTSITMMGE"
     gene            complement(22753..25371)
                     /locus_tag="PKJ16_02200"
     CDS             complement(22753..25371)
                     /locus_tag="PKJ16_02200"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HNV45823.1"
                     /translation="MHTDRRYKEIAGILVLMLSLIIALSLAGYHITDYAQLAENRPVK
                     HVLGPLGAAVGAGLRTAFGYPAYLIFAMTALAGWSVLRRGSAIFEIERIFALVYLAIT
                     SSALFALVSTSGVPQFAGGYLGVYVAVMLTRLAGSVGAYLIVIILNLIGLIFLGALSL
                     AFLSEGDRRTGDAFIRAWGVIAQAFATIASRITRRKTTAGPDTDATETRSGKKPPWIT
                     KKRIRIGDDDAALEKELALNTPRLLEMMEQTSRATAPDPFDALRETYRAGRARRIGPP
                     AVDDASDRAALANDAPDDEEIVFTPDEIQRPVTPGVVPNRITSIESEPAVRDAVPASA
                     MPDQAARRSTPAVALADPPVAVEEDEDDEDAADCIIAQSPHRDEMALDELGDDADGPA
                     PAGDAGVERVFDEIGIKSKYAIPMSYLKSSVAIDVESWKSEARRNSELLVNTLRDFGI
                     ESKVVRVNRGPVLTLYELQIAPGIKVNRIVALSDDIAMALAASRVRIVAPIPGKSAIG
                     VEVPNTAREMVTLGDIKLTEEYGTDAGRLKVALGKDILGNPVTLDIRRLPHLLIAGAT
                     GSGKSVCVNAIITSLIYNYDPNYVRFILVDPKMVELQLYDGVPHLLMPVIVEPHVVPK
                     ALKWAMYEMERRYKLLSALCARDIERYNEKAAASKTPHERLPYIVIIMDELADLMMVA
                     AKEIEGFITRIAQKARAVGIHLVLATQRPSVDVITGIIKANFPARIAFQVAQKTDSRT
                     IIDQNGAEKLLGKGDMLYQSPMSSFPIRVQGAYIAEDEIMAVVEHLKKLAKPAYINIE
                     ESIFDEDADDEDAVEDELFVEALKIVEDSKKASASYLQRRLSIGYNRAARIIELMEER
                     GYIGPQTGSKPREVFI"
     gene            complement(25639..26016)
                     /locus_tag="PKJ16_02205"
     CDS             complement(25639..26016)
                     /locus_tag="PKJ16_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45824.1"
                     /translation="MPAGRDGEIRITYLQTLPYGLFSDSATRELKKTGFDPDRHACIL
                     VKILASAKRKYYNSACYNTGATDDPCAPGNNIIYVVSKKDYPVFTRERYIDIRTVFLF
                     KSAVYGQSIPRGIYSFCTDYRGN"
     gene            complement(26162..27817)
                     /locus_tag="PKJ16_02210"
     CDS             complement(26162..27817)
                     /locus_tag="PKJ16_02210"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HNV45825.1"
                     /translation="MKAKLDKVRIVPLGGLGGIGRNMTVFEYGEDMIVVDCGIMFPSE
                     GMHGIDFLIPDFAYVAKNRDRLRGIIITHGHEDHIGGMPYLLQEANAPVYATRLTLGL
                     IQKRLEEKPPKEAPSFVEMSPRDKVHIGAFVIEFIRVNHSIIDGVGLAITTPVGTIIH
                     TGDFKVDLTSVEGEVTDMYRFAEYGENGVLLLMSDSTNAERDGFTRSERTLERKLMEI
                     LSRAKGRTIIATFASNLGRIQQVLNAAHKYNKKVLVSGTTMLKNIDIANSLGYLSFHD
                     DLFEDIRNANSLPHKRQVVLCTGTQGEPMSALSRIANGTHKNLAAGEGDIVVITASVI
                     PGNERMVYDVVNSLMRSGAEVYYEADADLHVSGHASREELKLMISLTKPKFFMPVHGE
                     YRHLRAHARLAESLAIKPANIIIAENGAMLELSAKSFEKTGAIELRDVYLDGREIGDM
                     GSSLIKERQAMAADGIIFITLFVSRGRLAAAPDIVSRGFTSQRLAEITAEISQDVEAL
                     CERYLDDGMKEADLSVALRKSVKNFIGKSLHKNPLIEVRVFGV"
     gene            complement(27814..28248)
                     /gene="dut"
                     /locus_tag="PKJ16_02215"
     CDS             complement(27814..28248)
                     /gene="dut"
                     /locus_tag="PKJ16_02215"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013650807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HNV45826.1"
                     /translation="MVHVKIKLDPGATLPAYQSAGAAGADVCARLDAPLVLAPGGRTL
                     VPTGLFLEIPDGYEAQIRPRSGLAIRHGITLLNTPGTIDADYRGELKIILANLGSEPV
                     SIENGMRIAQMVIAPVERASFVAAEVLTDTARGAGGFGHTGV"
     gene            complement(28301..29257)
                     /locus_tag="PKJ16_02220"
     CDS             complement(28301..29257)
                     /locus_tag="PKJ16_02220"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HNV45827.1"
                     /translation="MNSVSIAFESVAVPIGIGLIGFFVIARRMVPESVLTVLTPLVID
                     IALPCLIFAGMIRDFRPEAAPGWWQLPLWWMVFTALALAFTALATLLSRTKHRSEFAM
                     ALFYHNGLFVPLAFLTGMFGESSPHHIDLFLFTIAYPAFFFNTHHLFYPRKIRAAGAL
                     PLWARILNPVLVATMIALALRLGGIGDHIPGVAVSIAKSVGAMTIPLIMIIIGGSIYI
                     DFTAKGRFDLRESLVFVLCKNIALPACMLGTIALVRPDYNVALIMTLAAAVPPITAVP
                     IMVERLGGNRAIANQYLMASFGACVATLPAVFWAFTLLYPGR"
     gene            29432..>30968
                     /gene="carB"
                     /locus_tag="PKJ16_02225"
     CDS             29432..>30968
                     /gene="carB"
                     /locus_tag="PKJ16_02225"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV45828.1"
                     /translation="MPKRTDINKVLIIGSGPIIIGQACEFDYSGTQACKALKKLGYEI
                     VLVNSNPATIMTDPGMADRTYIEPLNAETIERIIEKERPDALLPNLGGQTGLNLSAEL
                     HKNGTLEKYGVRIIGVAADAIERGEDRIAFKDTMAELGLEVPRSAPAYSLEEAEKIAV
                     EIGYPLVVRPAYTMGGTGSGFVYNKEDLRVVVARGLQESLVGQVLIEESVLGWEELEL
                     EVVRDAKNNIVTVCFIENVDAMGVHTGDSYCVAPMLTISPELQARLQDYSYRIVEAIN
                     VIGGTNIQFAHNPKTGRVVVIEMNPRTSRSSALASKATGFPIALISSMLAAGLTLDEI
                     PYWRDGTLEKYTPSGDYVVVKFARWGFEKFAGVKDELGTQMRAVGEAMSIGKNYKEAF
                     QKAIRSLENGRMGLGFAKDFNRKSLDELVALASTPTSERQFIMYEALRKGADAALLHE
                     RTHIKPWFIQQMKELVELEERIIACRGGEIPDELLAQAKRDGFADAYLAKITLVLRVI
                     YLDA"
BASE COUNT         5421 a         9588 c        10208 g         5751 t
ORIGIN      
        1 cccacgttta tcgaaacgaa ggcgcacaat gccgtggagt cgtatgcgct ttcgcctgac
       61 ggcaccatcg ccacgacctt caccttccgc gacggctcct tcgacgggcc cgccaagcgc
      121 tacaccccgc gcggcttcgt cgtgaaccgg aagacgaacg cggagtgggg gatgcagttc
      181 atctggccca tcaaggggga gtttctcatc gtctatctgc gcgacgacta cggggtcacg
      241 atcatcgggc gcagcaagcg cgattacgtg tggctcatgg cgcgcacgcc ctcgatcccg
      301 gaggaggagt accgggcgct ggtgaaattc atcggcgatc gcggatacga cgtgggccgc
      361 ctctaccgcg ttccgcagaa atgggacgcg cgctgacggc gcgcggcgcg cgttcgggcg
      421 tcatgaaaaa atcttccatg ccgcgaacag cgcggtgtag atggcgagat agagccggta
      481 gaagttgcgg aagcgtatgt agtcgggatt gtcggggtac atggtcccga aaagcgcgcg
      541 cagctccttc acgaagaccg gctttatgtt gatgatccag ctgtcggttt ggtagacgat
      601 cttgcggaac cggctgcggt agtagcccag cggcagcccc cacgcgaaaa aaaggataaa
      661 ccaaatattt ctccgtacga aatcgatgac caggtccatt gcggcctcct agcggtcgta
      721 gcggtacacg atgtcgacga ccggattctc aagccttccg cccacccgac gcgtaagcag
      781 gctcttcccg tggatctcga gcaggcggcg gctcgcgctg tcgtagacga gctccaccgg
      841 gtccaccgcg atgcgcagga aaacgttcgc gatcttcatt tgaaacacct ccacgccggg
      901 gcggtttcgg ggcgatccgg cgatgcgcgt tatctgaaat cgcgcgagcc gctgcaggct
      961 catcgccggg agatggaagt acgccgtttc gccgcgaagg agcgaggccc agcgccggtg
     1021 cacgaaggcg gtgagcgtgg gccccgtgac gatgtccggt acgacgtcga cctctccggt
     1081 tttcggctcc ccgcccttgg tgtagctgaa gcggatccgg tttccggcgg tttcgagcag
     1141 gcagccgcag ccggtgtcgg gcatggtcac ttcgtatcgc tggatcgcgc cgtccttgcg
     1201 cgtcaccacc tcgcgcgcgg cgagcctgcc gtcgacatgc gtgtaggtgt gggtgagaat
     1261 ggtggcgccg tcctttttgg ctatggaatt tttctggcga aagatgagct gctgccggtt
     1321 cgttcccgcg agatacaggt cggcattgaa cagcgcctcg gcgcggacct ctccggcgac
     1381 ggtcccgata aggaggagca gcgccatcaa cattgccgct attgttcggt gtgttttcct
     1441 ggttttcata atgggacaag gctacttcgt ttggaaaata aatcaaaaaa aaattgtttt
     1501 tttgatccgc gcacggtaca gtgcttcgac aaagacaatg gcttgttttg gagggcctca
     1561 tgggcgagat actcaattca tattcgattg ccgcgatctt cgttttcgga tacatgaccg
     1621 tcctgttcct gctggccgtc ttcctcaagg acaacagtat cgccgacgtg gcctgggggc
     1681 cgggcttcat catcgcggcc gcggccgtga tgatcgtgca ggagaccttc ggcgtgcgca
     1741 acttcctgct gcaagcgctg gtgacgatct gggggctgcg gctcggggtc cgcatcttcc
     1801 tgcgcaatca cgggcgcggc gaggactggc gctacaagaa atggcgcgag gaatggggct
     1861 ccctctttta cctgcgcagc tatctgcagg tgttcgcatt gcagggcgcg atattgctcg
     1921 tgaacgccgc gccgcttgtc atcgccaatt ccgcgccggg cgacatcctc gtgtggaccg
     1981 actacgcggg gctcgccgtg tggtgtacgg gattcttctt cgagtcggtg ggcgactacc
     2041 agctcgaccg cttcatcaag gaccccgcga acaaggggac catcatggac cgcggcctct
     2101 ggcggtacac ccgccatccc aattacttcg gcgaggtgac gatgtggtgg ggcatcttca
     2161 tcatcgggct cggggccccg ttcgggtatc tcgggatcgt ggggccggtc atcatcaccc
     2221 tcatgatcgt gttcgtctcc ggcatcccga tgacggagcg cgcgatggac cacgacccgg
     2281 cgttccaggc gtacaagaag cgcaccagcg cgttcattcc cttgccgccg cggggatgag
     2341 cgcattccgg cgcgcgacgc cggatcgtcg tctccccgcg aatttgttga tccgtgtccc
     2401 gaacggttcg tatgcgcgga gtcgcccgcg gcgtcggacg atgaagcgtg gtatgcgcaa
     2461 tccaggagca cactatcgga aggagccggc ccatgttcgt ccaggaccat cataagaccg
     2521 cgtttatcca tcgcgatcgg cccatcagct accgcgagtt gttgtcgcgc ataacgtctt
     2581 ttgccggcgc gttccgcatc aagacggggg accgcgtggc gctgtgcgcg gagaaccggc
     2641 cggagtggat ctacgccttt tacgccatct ggcagaacgg cggcataccc gtcccgctgg
     2701 acccgaccgc cgcgccggcg gacctggcgg tcgttctccg ggattgccgt ccgtcgctcg
     2761 cgctgtgttc cggcgaatgc gccgggcggg tgaagcaggc gctgcgcacc cgcggcatgc
     2821 ccaagacgac ggtccgcacc ctcgaaagcc tccccgatcg cgcggacggg cccgccacgc
     2881 cggtgaccgg cggcgcgccg gacgacaccg ccatcatcat gtacacgtcc ggcaccaccg
     2941 gagcgccgaa aggcgtgatg ctctcgttcg cgaatctcgc ggccagcatc cgcggcgtgg
     3001 cctcgctggg catgctcacg gccgacgacc gcatgctggg gctcctgccg ttctatcaca
     3061 tcttcccgct gcaggggacg gtgatcgcgc ccatgcagat cggcgcgacg gtcgtcatgg
     3121 tcgccagcct ctcgcccgag gacatcctcg ccacgctgca gaagcaccgc gtgaccatgt
     3181 tcctgggcgt gcccaagctc tatgaactct tccacgccgg aatcgtacgc aagatcaggg
     3241 cgaacccggt cgcgtacggc ctctttcggg tgatgcggcg cgtgaacagc gtccgcgccg
     3301 gccgcgtcgt tttccggaag gtgcacgccg cattcggcgg gcaggtccat tcttttctca
     3361 ccggcggggc gccgatggac gcgacggtcg cgcgcgaact ctggtcgctc ggcttccggc
     3421 tcgtcgaggg ctacgggctc acggagacgg cgccgctggt cgccttcaat cccatcgacc
     3481 gcatcaagct gggttcggtc gggaagccca tggcggggac cgaggtgaaa atcgataacg
     3541 gcgaggtgct ggtccgcggc gagaatgtga tgaaggggta ttatcggaat cccacggcga
     3601 cggccggcaa gatccgcgac gggtggttcc acaccggcga cctggagcgt ttcgacgacg
     3661 atggctatct ctatcttgag gggcgcgtcg acgagatgat cgtgcttccc agcgggaaga
     3721 acatcaaccc cgatgagatc gagcgcgcga tcctctcgtc ctcgccgtat gtcgccgaga
     3781 tcggcgtgtt cgcgcaccgg ggacacctga cggcgctcgt ccgtcccgat ttcgcggcgc
     3841 tcgagcgcga cgggatcggg accatcgccg agaccatcaa gtggaaggtg atcgacgcgt
     3901 acaatgcccg cgtccccggc cacaagaagg tgttgggcgt cgtcattacg cgcgacgagc
     3961 tcccgcgcac gcgcctgggc aagctcaagc gattccgcct gcccgagatc gccgctgcgg
     4021 cgcgcccgtc gtcggggcgg gccgccggac cgacgacccc cgagtacggc atcctgaaac
     4081 ggttcctttc ctcgcacgcc ggcatggagg cgctgcccga tcaccatctc gagctcgatt
     4141 tgggcctcga ttcgctcgat cgcgtggagc tcctcgtgtt catcgagtcc gccttcggcg
     4201 tttcgctcga cgagcgcgat ctcgcgcagt tcacgaccgt ggcgcggctc gcggagctcg
     4261 tcgcccggcg caagacgcgc gcgaaggagg cggccgtcga ttggcgcggg atcctgcagg
     4321 cgtcggagcc gctgagcgtg cggggcggga tgtttctcat gcggatcgtg cgcggcgtgg
     4381 cgcggccgtt tctgcggatc gtcttccggc tccgctatcg cggactcgag catctcccgc
     4441 aggccccctt tatcctgtcg cccaatcacc agagcttcat cgacgtgttg ctgctggcgg
     4501 ttgcgcttcc cgaccgggtg ttgcgcaaca ccttctttat cgcgcgcgaa tcgctggcgc
     4561 ggcgggcgct cgtgcgggcc ctcacgcggc gcaccaatat catattcgtg aacttcgagc
     4621 gcgacgtgaa aacggcgctc caaaaggcgg cgtcgatcct caaggcgggc cgaaatctcg
     4681 tgatctttcc cgagggacac cgcacgcggg acgggcggat cgcccccttc aagaagacct
     4741 tcgccatcct gggcgccgag ctccaggtgc cggtggtgcc ggtcgcgatc cgcggcgcct
     4801 acgaggcgct tcccgcagcg ggacgccttt cgcgcccgag atccatcgag ctcaccttct
     4861 gcgcgcccgt gcggatcatg caagggaatt acgccgcgcg cgcggagtcg gtgcgctcca
     4921 tcatccgcgg cgtcgtcgag cgggcgtgag tccgccctga tcgcaacggc ctcgttcgca
     4981 tgaacgcgca tccgtcgagt tgcgaattcc gcctcaccct cggtgatggt cgcgccgtgt
     5041 tcgtgcgtcc catcgcgccg aacgatgcgc cgcttctggt cgatctcttc aatcggctct
     5101 cgccgcaatc gatttatctg cgtttcctga gtcgcctgtc cgggttgccg gcggacatgc
     5161 tgcaccattt cacgcatgtc gattacgatc gggagttcgc ccttgccgcg gtggtcgctg
     5221 aaaccggcgg ggacgccgtc attgccgtga gccgctactc gcatgatcgc gacaccggca
     5281 tcaccgacct cgcggtcgcc gtgcgcgacg actggcagca cctcgggctc ggccgcgaga
     5341 tgcttgcgcg catcgtcgcg atcggaaggg agcacgggta tcatcgctac gggagcatga
     5401 tggaccccga caacacggcc atccgggcgc tgctccatga gctcggctac cgcgtgaaat
     5461 acacgccgcg cgacgggtac tttgacgtcg agatacggtt cgattgagcg acgcaaagcg
     5521 agattgatga atcgatttcg attggcggac gaccgtcggt cccgatccga ttacgatgga
     5581 gtgaaacgat accaatggaa aaagcagaac tcagaaaaga cgagtttgtc gcgctcttta
     5641 cacacttcgg cctggagccg gattggaaat ttctgtccga aaacttcgcg acgattcccg
     5701 actacgagtg gaagggtttc ctcaattcga tacacggaat cctgatgcgg cgacaaaacc
     5761 gccgggaagg ctgaccgcgc gacgttacgc gtccgcctcg ggcaggcgtt tctcgtattt
     5821 cggctcgcgc agccccaggt cgacaagcat cccgtaggtg agggcgagct gcaggtttcc
     5881 gatgaaggcc tcggggatct cgcgcgcggg atcgaagctg cgcgcgaaat agtcgcgcgt
     5941 cgcgcgcagg atgtgccgca gctcggggct ttcggcgtgc gcggtgatga tcagctcgcg
     6001 aaaggtccgg atgtagtcgc ggtgccgcgc gatgtactcc gtcacgtccc gcgcgcccgt
     6061 gaccgcgccg aagtggcaca ggccgacggc cccggggtcg agcgcgcgga tcgtttccac
     6121 gccggccatg tagcgctcga acgagaaatt gggcggcatt gacgttgccg aggaaacaag
     6181 ttcgctgtcg tgaaaaagcg tcccgcacgc ctcgccggcg aagacgaact ccgtccttcc
     6241 gtccgagcgc acggccggtg agcagtggtc ggggcagtgg cccggggtcg ccatgagcgt
     6301 cacggagatc ccgccgtcga agtcgatcgg aagcgccgcg tcctttgcga cgatcgtgaa
     6361 gcactcctcc ggcgccgggt ccatcgtccc cacgaaatcg ccgaaggtgg tgcgcgcgcc
     6421 cgcgaggtgc tcttcggcgt cctgcagccg cgccttcatg tcggccggcg cgaagatcga
     6481 gaacgacggg ttgtgcgccc gcatgatccg cgccagcgcg ctcgcgcccc ccatgtggtc
     6541 gaagtgatag tgcgtcccgg tgattcccgc gacgctcccg agcggaaccc ccaggcgcgc
     6601 gattgcatcg gccactgcgg gcgcattgtc gctggtgccg gtgtccatga ccagcccggt
     6661 gcgcccgtcc cacaccagaa aggcggcggt cacggcgggc tttccgaact gcagcgtgtc
     6721 gatgaggtgg acgtgatcgg tgatccggcc ggcgtgcgtg ttgatgggca tgatggagtc
     6781 cgcctctgtg cgcggcgcca cgcccaatat tttgctcgac acggacgcgg cgcagaataa
     6841 aatgcaaaac caccattgga caaaacggcg gtttctgtaa agtggaaaaa acacggacac
     6901 ggcaggtgcc gatgaagcac acgcgaaaag ccgcgccccg ccccgcgcgc gtcggcgcat
     6961 tcttcgatct cgagaagacc atcgccgacc acgcggtcat ctgggagttc gtcatggagc
     7021 tctaccgccg gcgggagatg ggactttcgg tggtcgtccg cacgatgtgg atctatttca
     7081 agtacagcct gggcgtcgtg aagaatttcg agacggtgat gcgcgacgcc tcgcgccgtg
     7141 cgtttcaggg acggaacgca gcgcgctaca tcgagatctt ccgcgagctg ttcgatgagc
     7201 ggctcaagca ccgcatctat ccgggcgcgc gcgatacgat cgccgagctc aagaagcgcg
     7261 gggtgcgcac ctacatcatc tcgaccacgt tccagtcgct cgtggagttt tacgcgaacc
     7321 atctgggcat cgacgagttt tacgcgacca gcctcgagat cgtggacggc atccacaccg
     7381 gggcgatcaa cgggcccatc tactactacc agcacaaggc gtcgctgatg atggagatcg
     7441 ccgcgcgcga caacatcgac ctcgcacgca gcttcgcctt cggggacaac ctcaacgacc
     7501 gcgtgatgct cgagtgcgtg ggacacccgc acgcggtcaa tcccgacaaa aagctccgcg
     7561 cggtcgccga gcgcggcggc tggaccatac tatcctgggg gatatagcgc atggacaagt
     7621 ttttctatcc cgaaagcatg gcggtgatcg gcatctcgat cgggcggatc acgctgggca
     7681 acatcatcct catgaacaac gtgaacctgg gctaccgcgg ccggctctac ggcgtgggca
     7741 aggaggcggg cgagctgcag ggcgtgccgg tcttcaccag cgtggacgag ctccccgaaa
     7801 cgccggacgt cgcgatcatc atcacccccg cgaaaacggt gccgggcttt ttgcgcgact
     7861 gcgcgcgcaa gggcgtgcgg catgtggtga tcgagagcgg cggcttcagc gagttctcga
     7921 cggctcactc gacgctggag gacgaggtgc gcgccatcat cgccgagcac ggcatccggc
     7981 tggtggggcc caactgcgtg ggcgcggcca acaaggaaat caacatgatg atggccttcg
     8041 gcttcttcaa gaacgagggc ccgcccagcg gcgtggccgc catcacgcag agcggcggga
     8101 tcggcaacac cctgatccgc atcgtgagcg acaacaatat ccacttcggg aagttcatct
     8161 ccatcggcaa caagctcgac ctggacgagt cggacttcat cgagtacttt ctgcgcgacg
     8221 agaagacgga catgattctc atgtacctgg agggcttcaa gcgcggccgg ttcttcttcg
     8281 agctggcgat gcgctcggac aagcccttcg ttgtcctcaa gtccaaccgc agcgagctga
     8341 gcgcgaagat cgcgcaatcg cacacaacgg cgatctccgc cagcgacgac gtggtggacg
     8401 ccgccttcgc gcaggccgcc gtcacgcgcg tggagggcga ggacgagctc accgtcgcgg
     8461 ccaaggcctt ccaactcccc gtgatgcggg gacggcgcgt cgcggtgctc tcgcgctccg
     8521 gcgggcacgc cgtgatcacc gccgacgcat gcgcgaagta cgaattcgac atggtgcagt
     8581 tcccggcgtc gtacatcgag aacctgaaga ccatctacac cacgcgcgtg atcgcgcacc
     8641 agaacccgct ggacctgggt gagattttcg actacacgat cttcattaag atcctcgaag
     8701 agaccctcaa gctcgacggc atcgacggca tcatctttaa ccacctgttc cagtcgggct
     8761 acgagtccga atcgtcgcgc accttcctca cggcggtgaa ggacctggtg gcccagtaca
     8821 aaaagccggt cgccgtgtcc atcatgacca acgccgagga gcttttggac atcaacaaga
     8881 accatccgtt cccggtcttc gcctcgcccc tgcaggcggc cgcggcgctc tcgatgtcgc
     8941 tggcgtatca tgagcgaaag accgcgcgcg atgcgcgggg gacggcgccg tcgtttcccg
     9001 tggacgccgc ggcgatcgag gccatccgtg tccgcgccgt cggcgagaag cgcattccgc
     9061 tcaccgacga ggcgcacgcc gtgtgcgcgg ctgggggcat cgcctcgccg gccctgcgcc
     9121 gcatcgccga cgaaaacggc gcgcggggga tcgatctccg cttcccggtg gtcctgaagc
     9181 tcctttcgcg cgacgcatcg cacaagtccg acgtgggcgg cgtgcggctg gggatcgagt
     9241 cggccgatgc gctcgccgcc gccgtctcgg agatgcgctc cgcggtgtcg ggcgcgccga
     9301 cgccggtcgc catcgacggc ttcctggcgc aggagatggc gccgcgaggc gaggagttct
     9361 tcgtgggcgc gcggcgcgat ccggtcttcg ggcccatcgt ggtggcgggc tacgggggga
     9421 tcttcatcga gctcttccgc gaccgggcga tccgcgtcgc gccgcttacc gcgagcgagg
     9481 cgcgcgacat ggtgcgatcg ctcaagacct atccgctcat cacgggcctg cgcgggcgcg
     9541 cggcgctcga cgaggacgcg ttcgtcgagc tcatctgccg cgtctccaat ctcgtcacgc
     9601 gcgtcgagtc gatcgccgag atcgacctca acccggtgat cctgcacgag cgcgggaagg
     9661 ggctgtcggt cgtggacgcg cgcgtctttt tcgcgtgagc ggtccgctgc gcccatgaag
     9721 gagatcacct acgcggaggc gcgcgcgctt gcatcgccgg tcttcatcga cgtgcgcgcc
     9781 cccgtcgagt tcgtgcagga ccacatcccc ggcgcgatca atattccgct cttcgacgac
     9841 ctggagcgcg ccgaggtggg caccatctac cggcgcatcg gaagcgacga ggccatactg
     9901 cgcggcagcg agatcgccgg cggcaagctc ggcgagatcg tcgcgcgcat cagacggttg
     9961 ccctcggata atctggtgat caactgcttc cgcggcggga tgcgctcgag caccctggcg
    10021 gccattctcg attcgctcgc gatcaaaaat atctacaagc tcgaaggggg atataaggaa
    10081 taccgccggc acgtgggcgc ggcgctcgcg gcgcaccggt tctcgcagcc cgtctacgtg
    10141 gtgcagggcc ttaccggcac ggggaagacc gcggtggtgc gcgcgctcga acacgcgatc
    10201 gacttggagg agctcgcggg gcaccggagc tcggtcttcg gcgccatggg tatggtcccg
    10261 cattcgcaga agcgcttcga gagcctgctc ctggcgaagc tcgagtccat gcgcgacgcg
    10321 ccgtacatcg tcttcgaggg cgaggcgcgc aagatcggcg atctgtttgt ccccgactgc
    10381 gtagtgcgcg cgatcgccgc ggcgccgacg atcatgctcg tcgcggacat cgagcggcgc
    10441 atcgacatca tcctcgagga gtacgaggcg cacatcgatc cggccgcggt ttccgcgatc
    10501 gtgcgctcgc tcgaggggcg catcggaaag aagaccgcgg ccgagctcct ggacgcgctc
    10561 gacggcgggc ggtaccggga gttcgtccgc gtcctcctgg agcgatacta cgacgcgcgc
    10621 tacgcgcatt cgttcgcggg gtgcgacatc atcgccgagg tggagaacgc ggacaccgcc
    10681 gcggcctgcc gcgcgatcga gcggatcatc gccgaacggc cgggacgcat gtaaataccg
    10741 gcgtcgtggt gatcgcgcga cgcgccacac gagacataat ccttcacgcg cagcgcatca
    10801 gtattttttc gaacgccgca aaaaagacta aattttttct cctctgcgcc gatactagta
    10861 ttaatcattt tacactttgg gtcttcctta tggtgccgaa gcggaattat aaggtaagat
    10921 acaagatcac aaaacgggat cgccagatcc cgtttttttt tggtgcgcct caaatcaccc
    10981 ggatgatctc cttgatgccg tagatcgcct cgatgtactt gcgcgcgggc gtgtcccaga
    11041 agtagatgga gcgctctgcg ttcacgcgcg ccttctgcag cgtgaggtgg tcgttggcgg
    11101 tgaagaagcg gtgcgcgcgc tccatcgcgt cgtagaaggc ccacggggtg aagtccgaga
    11161 aaaggaagcc gttgccctcg atcgcgtcgt cgcgcacgcg gacggggaag acggtgtcgc
    11221 gcaggccccc ggtcgcgcgc gccaccacca ggcaaccgca ggcgaagccc tcgagctgcg
    11281 agatcccgcc gggctcgttc atggacggca tgccgaagat gtccgaggcg agcagcggta
    11341 tgctgatgtc ctggaagccg aaggagaccg agacgcggtc gcggtaatag tcgccgagga
    11401 ggtacagacc gtgcgcgatg tcctcgccgc gctggtcccc cgaggagacc gcgccgccga
    11461 tgatcgcctg atagcccagg tccctgaaga tgccctccga cgcgtccagc aggagctgaa
    11521 agcccttctg ttcggtgatg cgatggatca tcgtgaacag caccgcgcgc ggatcgacgg
    11581 tgaaaccgcg ctggagctgc aggagcatct tgttgagcgt gcggacggtg ctgccgcgcc
    11641 ggagcgcgtc ggggatggcg cgcacggcgt ccagccccct gaggatctcg gggaaatcgc
    11701 gctcgacggt cgccgcgagc ttcgcgtcgg tgcgcaggtg ttccttgaat gccgggtaat
    11761 gctcctcctg gaagccgctc gccgcgaagt tgcggtcgat gcgctcgcgg aagtcgcgtc
    11821 cgatggcgtt ggagatcccg atcacgttat ggagaatgcg ctcgagtccg tcgcaggcgt
    11881 attcgatctg ccgggcgtag gacgggctca cggtgatggt gcggtcggcg aagcggatgc
    11941 ccgccgccat gcagttgagc cggtccccga agaccgggtc ggagaaatcg cccgccttcc
    12001 acgagggaag gttgaacagc gagaagaggt cgaacccgcg ctcgaagcga tgataggcgt
    12061 cgaagtactg ccacccgccg ttgtggatga tgtgcaggaa ggtggtgcgc cggaagttgg
    12121 gattgtcgtt gtagacgtgg tcgctgcgca cgatcccgtc gaagatcccg gcataggcgt
    12181 cgttggtgaa ggtgaacaac ggcttcaggt tgaagcaaca gatcacctcg gcgcaggcgc
    12241 gcgcgaaggc gatccggcgc ctgagcttct cctccgcggt atagccccag tacagcccgt
    12301 cgaagaactc gtggtggtcc aggagaaagt aggtgatgcc gtccacctcg ccgcggtgga
    12361 cgcccacgtc gtaatcgtgg tccatgatct tgaaacgcac gttcatgccg gcgtagcgca
    12421 cgccgtactt cgtcgccgcc tcgcgcatct tccgctgcga cttgtcgtcg ccgctgcggt
    12481 agtagcccgt gatgacgtac acctcctctt ccaggttcac cagctcccgg gggagctcgt
    12541 acaccacatt ggccaggccg ccgctcttgg agaatggctc ggcctcggcc gacatgaaca
    12601 cgagcgagcc ggccgcgctg tggtgcaaga gcgccgcgcc cagctctcgc aggcgctcgt
    12661 cctcatgccc cgcgaggcgg ttcatgtagc cgcgtagcgt atcgccgtcg aacaggccgt
    12721 ttttaaacgc atgatagagg ctccgcttga gtccgagctc cagcgtgggc cggtgatccg
    12781 cccagaagag cgggagcagg tggctgatga tgaagttcgc cagcccgtcg aaatcgtgca
    12841 gcgccgctgt tatctcgcgg aaggcgaagc tcgccgtgaa atgggccggg acgccgatcg
    12901 cgcagagccg gaagaaggaa tcgcgcagaa agtcctggta gtggcgctcc agctggtcca
    12961 ccgtctccag gcgcaagagc ttcacgcgct cggtgaatga gaccaccgtg tttacgcgcg
    13021 cggtcttgag ctcccgcccg gtgaagtagc tgtaggtatt ggtgatgggc gagtagagcg
    13081 ggtcgatcac cttgaagagc gcatcgtcgg cgatgggcag cacgggattg tcgaactgga
    13141 tggaggcgtt ctgattgctg tcggcgaaat tgaacacgcc gatccaggtg tccgtcgccg
    13201 tgaacttcat cgccgcggcc accatggtgt tgttcgccca gacgagatgg cccttcccgg
    13261 tggccgtgcg gtgaaaatcg taccagtcgc ggtagaactg ctccacgctg cggtgggcgg
    13321 cgtattcgaa ctggttccag ttgacgtaga tgttgtccag gaagaccttc cagccctcgc
    13381 cctcggcgct cccctcgtag tccatcacga tattgctcat gaacatggtg agcgcgaccg
    13441 ccgcgcgcag cccccggtgc ccgaaggtgt tgagcgcgcg ccgctcgtcg tggttgccca
    13501 ggatccccag gagctcggtg ccgcgcggca gcgacttcgg gaaatagttg tcgtagatgt
    13561 ggtagatctc gcgcacgtcg gtttttccgt gcacgatgtt ctcgcagatc ttgaacagcg
    13621 ccgagttgta cgggatgagc cccaggcgcg tgagaaagcg ctcgtggccc cagtagcatt
    13681 ccgcgatgtt gatgaagaag cgctttcccc gcgcgcgcac cgcgcgctcc accgcgagca
    13741 tgtagtagta atgcagcggc acggccagga tgtcgcggca ggccgagtcg tagaagccgg
    13801 tggtcacgaa gtgctcgtcc tcgcggtcgt tgacgatgat ggcgccctcc accatatcct
    13861 cgtcgctgcg cgcctcgtcg taggtgaaca ggtagttgtt ccgcttcatc atgatgggca
    13921 cggcgtgcgc cgagtcgaag cggatgccgt cgatgtcgta ctcctcgatg agcgtcacga
    13981 tggaatcggc gagccagtcc cacacggcga gcttgcggta gttgaacagc cgtccgtcgt
    14041 cccagcttcc gtagcggccg tccgtcgagg cgcggaagac cagggcgccc ccgccgtcgt
    14101 agcagcgcac ggcgcagtcg tcggggagca cgtcgctcat ccggttcagg tgcggcacga
    14161 cgtccacgat caccttcacg tccagcgcgt gcgcccgggc gacgagcgcg cggaactcct
    14221 cggggccgcc cagatagggc tcgatctccg tgaggctcat gggggagaag ggcgagggcg
    14281 aggtcgcgcc cggcgcccag tcggaccggt tggtcccgcg ccgctgcgcg cccagcaggt
    14341 atattgccgt tatgcgatat cgttccttga tgtcctccag gtgcgccgcg atgtccgcga
    14401 agcgccccag gcggatcacc tcgccgttct cgttgtacac caggcccggg tggccggacg
    14461 ggtcgcgcca gtagatgccc gtgtgtccgt ggatgtcggg gaagatctcc aggatgatct
    14521 cgccggccac gttgggcagt acgttgatgc ggccagaggc gcccgggagc gtgagccatt
    14581 cggcattttt caccttgcgg cgatagacga ggtagtcgta gtggccgtac tcctcaaacc
    14641 ggcgctcggc gatgaagacg gcccccgatt cgtccggagt gagctcgatg tcatcgaagg
    14701 cgatctcgtc ctgggtggga atgcgcccgg cggcggcgtt ccggtagatg cgtacgaagt
    14761 agccgccgcg cgagtcgggg agcgagaagg cgaagcggtg cgtctcgccc acatgcacgt
    14821 tcacgaactc ggtggccagc ggcgcgtcga actccttggg ggccaggacc cgctccagga
    14881 gcatgttggc cttgttgcgg acctcgtgga tgtcgtggcc ggaggcggcc acgagcagcc
    14941 gcatcacctc gcgcagggag gcggcgtcgg aggttttctt gtagcggcgc gccaggcgtt
    15001 cgagcgaggc gatggccttc aggttcttgt cccagtgaaa gttgagctcg tcgcccttca
    15061 cgcgcgcgtt gaggaggcgc agatggctct ggcggttgcg ctcggtgaag cccttgagcg
    15121 cgcgctccgg gaccgccgcc tcgggctcca cctccgtgac ggcggagatg cggatcacct
    15181 cgtcgaggag gctttgcagc gcgcgcgccg cctcgggcga gaagacctcc gtgtgggcgt
    15241 aacccgtctg gatggcgcgt tcctcgatct tgagcaccag gcggaaggcg tcgaagagat
    15301 cgcgcccgcc ccgcttctcc tcggcggcca gcgcgtcgaa gacattgagc cccaggtaga
    15361 tggtggggat ttccgtccag tgcgggccct ggccgacgtg cgcgagcacg gtggtctcgt
    15421 tcgagacgtg atgcagcgtg ggcgcaccgt ggcgctcctt gaccaggacg aaccgccgcg
    15481 cgagcaggct caggttgttg tccgcggcga tggcgcggtg cacgaaatcg accgcggcga
    15541 cgatgcgcga agacagccgc gcgtcgctgc ccagccgcgc gaggatggtg cggtcgtcgc
    15601 ccgagacacg gccgaacgcg gcgttcttga tgtcgatgta gcggtcggcg agcgtctcgg
    15661 gcccgtcccc ggcgttgatg agcttgacca ggtcgtgcga ggggaagaag tcgaaacagc
    15721 ggttctcggc gaaggcctgc cggagttcga tcttgaccag ctcgtccgag atctgccgct
    15781 cctcgcggag cgtgaatcgg gagaggctgc tttgcgtgct ctgggcggcg tccacggcgg
    15841 tcacttcgtg tcgatgagcc ggaggaccgg gtagcgcttg ccgcgttcgg tgtgcagcga
    15901 caccggggtg tagatgaaat tgatccgcgc gcgctccttg atcgattcct tgatgtcgtc
    15961 caccttctcc tcggggacga tcacgtagag gcggctgcgc ccggatttcg tgctcatcag
    16021 cagtacggcg tcgtcctcgc cgccatggcg gcggagcgcg cgcggcgggc gcttcacgaa
    16081 gcggaccggg tacagcgatc cgcggaactt ctcgtccttg aggtcgcgat agtcgtggac
    16141 gagcatcttg taggtcgccc gcggcagttt gccgaaatcg ccgccatcaa catcgcttcc
    16201 tttacggtct ttgattgcaa tgtccttgcc cgtgtcgagg ggctgggccg gccggctttt
    16261 aagcaccaga aaggtctgaa tggacgggtg cttctcctcg ccgccccggg gcatgatgac
    16321 gtctccgccg ttcgcgcgcg ccacccgctt ggcctctgcg agccgcttgt cggccgaact
    16381 gctgcgcacc tcgacggtgc cgatggtatc gtaggcgccc gtgtcgccgt agggcacgac
    16441 cgcgaccgcg gcggtgtccg gcaggggcgt ccgtgcggcg ccggtgagcg cgaactggct
    16501 gcgcgcggcg caaccggcga acaaaatgac gaaccagaga cagaggcatc gacgagccat
    16561 ggtgcgacct cccatgtggt tttggacaga cactgccagt ttttccccaa cgcgtcaacc
    16621 gaaataccga cgttgcgagc agtgcgccga atcgattgat ttcttgccgc gatgggcgcg
    16681 cgtgcgggac ggggcgcccg ggctctcaaa taaaatgctt gccagccctt tcgggagctc
    16741 ctatcatgct gtcgcagcgg aataggtcaa tgccccatga acacaaagat ggatccaacg
    16801 actgaccgtc gtcgtcaccg gccatcctat attgggcggg ccttcttcag cctgctgttg
    16861 ctctggatcg tcctcgagta tcgcttcacc tggttgttcg tccccgccat cggatttatc
    16921 gcctacgcat tcgtctggat gctgctggtc gagttcggcc ggcgcgtgcc ggtggaccac
    16981 atcgcggtca agttcgtccg caccgtgatc gacatcgcct tcatcacctt cttcgtgtat
    17041 ctcaccggca cggtgcagac cttcctgctc atcatatatc ccgtctgggt ggccggcgat
    17101 tcgatgaacg tgcgtcgcgt gtacggggtc tttacgacgc tcgtcgcgtc ccttgcctat
    17161 atcgccctgg tcctgctcga gtacttctcg gtcgttcccg acatcaacat aatcaccgaa
    17221 acgccggcgc acatcggcac gttgcagatg ggcgttttca tcgtgctcat ggtcaccatc
    17281 aacttcggcg tgcatgcgct ggtgtacggc ctctacaagc ggctggccga caagaacgag
    17341 gagctctcgc gcgccaacgc cgccatccgg cagtcggagg cgcgctatag gaccatactc
    17401 gaacacatcg aggagggcta tttcgaggtc gatctcacgg gccgcatcac gttcttcaac
    17461 gccgcgctgt gccgcatcag cgggtatacg gccgaacagg tcgacggcag aagctatgcg
    17521 tcgttcatgg atcccatctt cgtccccggg atattcaaaa ttttccaccg cgtctacgag
    17581 acggagcagc catccgaact cttcgactgg tatttcgtgc accaggacgg aacgcggcgc
    17641 atcgccgagg ggaccgcctc gctgattgtc gatgccgagg gcgcaaagat cggtttccgc
    17701 ggcctgttgc gcgacatcac cacgcggcgc gccgccgagg aggcgctgcg cgcgtccgag
    17761 gaaaagtatc gcaacatcct cgaagggatc aaagagggct attacgaggt cgatctcgcg
    17821 ggggccgtgg ttttctgcaa cgattccttc tgcgcgatct tcaacgcgcc gcgcgagcgg
    17881 gtcgtgggca agaactacgc gagcctcatg tcgcccgaga gcgccgggcg cgtcttcggc
    17941 gccttcaatg acgtcttccg cgagcgcgac caccgagagc tggtcgactg ggagatcacg
    18001 ctgcacaccg gggagcggaa gttcctggtc gcttcgatcg acatcatcgc cgatggatcg
    18061 gggcgcatcg ccggcttccg ggggatgatc cgcgacaata cccagcgcat gcttgccgag
    18121 gaggcgctgc gcacatccga ggagaaatat cgggacatac tcgaggggat ccgcgagggg
    18181 atctacgagg tcgatctcac ggggagcttt acgtttttta acggggccat atgcaccatg
    18241 tgggggcggc cgcgcggccg gatggtgggg ctcaactatc gaaactactt ggaccaggac
    18301 gccgctcggc gcgtgcgcga ggtgttcacc gaaacctacc gcacgggcaa gcggaattat
    18361 cactccgact ggatcgtcac cggcgacggc gagcaacggt acatcgaaat ctccgtcgag
    18421 ccggtcgtgg gccaggaggg aaccgtgctg gggttccgcg gattggcgcg cgacatgacg
    18481 gagcgcaagc gcgccgagga ggcgttgcgc gaaagcgagg agaagtaccg gcacttcgtg
    18541 gagaacgcca ccgacggcat cttccgcaac gacgccaaag gcaatttcct gttcgtgaac
    18601 ccgtcgggcc tccggctgct cggctacgac gacaccgaaa tcacatcgat caactaccgt
    18661 tcgctggtgc tgcccgagta ccgcgatttc gtgaccgcgg cctaccgcag ccagatctcg
    18721 aacaacgtgg ccgagagcta tctcgagttt cccgtcaatc gcaaggacgg gacggtcgtg
    18781 tggctggggc agcgcgccac gctcgagcgc agcgcccgcg ggacctgcga gttccacggg
    18841 atctcgcgcg atatcaccga gcgaaagcgc gcggaggacg atctgcggcg cagcgaggag
    18901 cgctaccgga cgatcctcga gagcatcaat gagggctact tcgagctgga tatccgcggg
    18961 agttttacct tcttcaacaa ggcgctggcg gagatctggg ggcgtccgcg caacgtcatc
    19021 atgggcatga actaccgcga gtacctctcg gacgaagacc gggagcgcgc ctacgcggtg
    19081 tataatgagg tgtaccggac cggaaaccgc ggcgccttcc tggattacga ggtcatcagg
    19141 ccggacggca gcacgcgcta tgtcgattcg tcgttcgacc tcatgcacac gcccgacggc
    19201 acgccggtcg gtttccgcgg tatggtgcgc gacatcaccg agcggcgaca ggccgcagag
    19261 gcgctccgcc ggagcgagga gaagtaccgc gggattttgg agaccatcaa ggaggcgtat
    19321 ttcgaggttg accttgccgg atcgttcacc ttctttaacg gaaccctgtg cgaactctcc
    19381 ggctattcgc gtgaagagct gatcgggctg aattaccggg aatacatgga cgcggggacg
    19441 gttgcgcggg tgttcgagac gttcaacaag gtgttccgca atcgcaccgc cgagccgctg
    19501 ttcgactggg acatcgtccg caaagacggg ggcgtccgat cggtggagtc gtcggtggac
    19561 ctgctctatg acgccgccgg gaatcccgcc gggttcaagg gcatcatccg cgacatcacc
    19621 gagaaaaagc gcgccgagga ggcgttgcgg gagctcgacc ggcagaagtc cgacttcttc
    19681 gcgaacatct cgcacgagat ccgcacgccg ctcacgctga tcctggcgcc catcgaatcg
    19741 gccctccagg gcgattacgg cgagacgatg gaccgcggca tgctcgtgaa cgtgcaacga
    19801 aacgccatcc gtctgctcaa gctcatcaac aatcttctcg atttttcgaa gatcgaggcc
    19861 ggccgcatgg ccatgaaggt ccgcgaggcc gacatcgtcg cgtttattcg ccggtacgtg
    19921 gacacggtgc ggccggccgc ggaggcgaag ggagtcgcgc tcgatttcga gtccggcgcg
    19981 ggatcgctgc cgctctcgtt cgacgccgag aagatcgaca aggtcttcat gaatcttttt
    20041 tcaaacgcgc tcaagtttac cgatcacggc gggcgcatcg ccgtgcgcat cacggccgat
    20101 gagcgaaact gctatatcga gttcgccgac agcggcatcg gcattcccgc cgagaagctc
    20161 gatgtggtgt tcgaccgttt tgggcaggcc gcggtcggcg cgaaccgccg gtacgagggg
    20221 accggcatcg gcctggcgct ggccaaggag ttcgtggaga tgcacgacgg catcatcacg
    20281 ctcgtgagcc gttgcatcga cgagcacccg gacgatcacg ggagcgtttt taccgtgacc
    20341 ctgccgcgcg ggatggagca cttcgaggga cggcgtggcg tggagttcgt gaccgccgac
    20401 gagctcgacg agtccgtggc cgatcatcgc tttgcgggga tgcgggagat gctggacctg
    20461 cgcgaggatc tctcgggcgc ggcgcccgag cccggacccg cggccggcgc gctcgcggac
    20521 aatcgtcgga ccgtgctggt ggtcgatgac aatgccgaca tgcgcgcctt tctccgtacg
    20581 cttctctccc gctcgtatgc ggtcgagctc gcggagcatg gagaggaggg gctcgcgcgc
    20641 gcgcggtcgc ttcggccgga cctgatcgtc accgacgtga tgatgccggt gatgaacggc
    20701 tacgagatga cgcggcgcat caaggccgac gccgagctca tgcacacgcc ggtgatcatg
    20761 ctcacggcgc gcgccgaggt cacgcaccgc atcgaggggc tggagttcgg cgcggacgat
    20821 tacctcacga agcccttcaa ctcgaaggag ctcctcacgc gcgccgcgaa cctcatcacg
    20881 atgaaggcgc aggagcgcga gctgggcatc gcctacgaga gcctcaaccg cgcctacctg
    20941 cagatgaagg aagacctgct gttggccaag accatccagg cgaacatcct gccggcgaac
    21001 gactcgacgg cgtgcgggct gtcgctgcat gtcgagtatc ttccgctcat cgaggtgggc
    21061 ggggacgtgt acgacatctg cgagatcgcg ccgggcgtgg tgcgcgtgtt cctcgccgac
    21121 gcgatcgggc acggcgtggt ggcctcgctc atcaccatgc ttatcaagag cgagtacgac
    21181 atgctcaagg cctcgggcgc gagccccttc ggcgtgttct ccgcgctcaa cgacgtcttc
    21241 ttcatgacgt atcgttcgct caaggtgttc ctgaccggcg tgatcatcga catcgacacc
    21301 gcgcgcggcg tgatcaccta ttcgtccgcc ggccacccgg tgcagtatct gctgcgcggc
    21361 ggcgagacgg ttccgctcgc gaaaaccggg cgcgccgtgg gcatctgggg cgggatcaac
    21421 tgccgggacg tggaggtgcc ctatcgggcc ggggaccggt tgctgctgtt caccgacggc
    21481 atctacgagg agttcaacag cgaaaacgag gagttcggcg aggaccgggt ccgcgggatc
    21541 atcgaagcgc atgccgggga gccgatacgg tgcgtgattg acgcgctcct cgaggggatg
    21601 accgggttcg tgggcgacgt cgggctcaac gacgacatca cggtgctggg agtcgaatag
    21661 gttttttaaa ggagcgccat cccgtgaacg gcgcctccgg tccggccgat gccgtgaaag
    21721 ctatttggac cggaactctt ccggaacaaa gtcgtccacc cagaggtcct ggatcgattt
    21781 catcacctcc ttcatggtca tgaacgcgaa tgtgccgctg ttttttttgc gaactataag
    21841 gagctgatcg ccccgggcaa tgtcgaggtc ttttctgagt tctacgggca gggcgatttg
    21901 ccctttgtcg ctcaccttga cggtgccgta tacgatcatc tcttcttcgc ggttctgctt
    21961 tttcatcggt caccccgtgg tgataggcat tatgacgcat aatgcatggt ccggctcgag
    22021 tgtcaagcga atgcgaaaaa tataatatat cttacttttc ttacttatta gaattgactt
    22081 ttcatcgggt caagcgatca ttcgcccatc atagtgatgg aggtgtcgat atgtataccg
    22141 ttttgcttcg tgtcgggggc gtttttcacc tgctctgcgc cgtaacgcat attttctttc
    22201 cccggatgtt ccagtgggac aagaacctcg cgggattgac cggtgagagc ctggccgtca
    22261 tcagatcgaa tctcaatgtg atgaacgcct gcctgttgtt tttctgggtc atgctggcgt
    22321 atctccccat cgttcacgcc cgggaaatgc tcgaattgaa aatcggcaac gcgctgctgg
    22381 tcttcgttgt gcttttctgg gttctcagga ttttcatcct gcagccgctt ttcattgggt
    22441 tttccgccga agggaccgtc ggttcgaccg tgatattcat tgtcgggttg ttgctgttcg
    22501 ccatcccgac ggtgaatgcg ttgtttcgga aacaaatatg acgggcggag tatcgttttt
    22561 agcggaggta atgttttgtc ggagttctgt ttctgtcgca ataacggcgg ggcggtgata
    22621 gactttcatc gaatgaagtc ggatgtactc ataaagaaaa cacgatgatt gcttgacgcg
    22681 ccaatttcgg atcgccggga ttcgctcttt tggttcggca gtgtggatag ccgtggggca
    22741 accgcgcggg aattaaataa acacctcgcg cggcttgctg cccgtctgcg ggccgatgta
    22801 gccgcgctcc tccatgagct cgatgatccg cgccgcgcgg ttgtagccga tcgagagccg
    22861 ccgctgcaga tacgaggccg acgccttctt gctgtcctcc acgatcttga gcgcctcaac
    22921 gaacagctcg tcctcgaccg cgtcctcgtc gtccgcgtcc tcgtcgaaga tgctctcctc
    22981 gatgttgatg tacgccggct tcgcgagctt cttgaggtgc tccaccaccg ccatgatctc
    23041 gtcctcggcg atgtaggcgc cctgcacgcg gatgggaaac gagctcatgg gcgactggta
    23101 gagcatgtcg cccttgccca gaagcttctc cgcgccgttc tggtcgatga tggtgcgcga
    23161 gtccgtcttc tgcgcgacct ggaaggcgat gcgcgccggg aagttcgcct tgatgatgcc
    23221 ggtgatcacg tccaccgacg gccgctgcgt ggccagcacc agatggatgc ccaccgcgcg
    23281 cgccttctgc gcgatgcgcg tgatgaaacc ctcgatctcc ttcgccgcga ccatcatgag
    23341 gtccgcgagc tcgtccatga tgatgacgat gtacggaagc cgctcgtgcg gcgttttgga
    23401 cgcggccgcc ttctcgttat agcgctcgat gtcgcgcgcg cacagcgccg agaggagctt
    23461 gtagcgccgc tccatctcgt acatggccca cttgagcgcc ttgggcacca cgtgcggttc
    23521 cacgatcacg ggcatgagca ggtgcgggac gccgtcgtag agctgcagct ccaccatctt
    23581 ggggtccacg aggatgaagc gcacgtagtt gggatcgtaa ttgtagatga ggctggtgat
    23641 gatcgcgttc acgcagacgg acttgccgga gccggtcgcg cccgcgatga gcaggtgcgg
    23701 caagcggcgg atgtccagcg tcacgggatt tcccaggatg tcctttccca gcgcgacctt
    23761 gagccggccg gcgtcggtcc catactcctc ggtgagcttg atgtcgccga gggtcaccat
    23821 ctcgcgcgcg gtgttgggca cctccacgcc gatggcggat tttcccggga tgggcgcgac
    23881 gatgcgcacg cgcgaggcgg ccagcgccat cgcgatgtcg tccgacagcg ccacgatgcg
    23941 gttcaccttg atgcccggcg cgatctgcag ctcgtagagc gtgagcacgg ggccgcggtt
    24001 cacgcgcacc accttggact cgatgccgaa atcgcgcagc gtgttcacca ggagctcgga
    24061 gttcctgcgc gcctcgctct tccagctctc cacgtcgatc gcgaccgacg acttcaggta
    24121 cgacatgggg atcgcgtatt tcgatttgat gccgatctcg tcgaagacgc gctccacgcc
    24181 cgcgtcgccc gccggtgcgg gtccgtcggc gtcatcgcca agctcgtcca gcgccatctc
    24241 gtcgcgatgc ggggattgcg cgatgataca gtcggccgcg tcttcatcgt cctcgtcttc
    24301 ttcgacggcg acgggcggat cggcgagcgc gaccgccggc gtcgagcgac gggcggcctg
    24361 atcaggcatc gccgacgcgg gcactgcgtc cctgacggcg ggctcggact cgatggaggt
    24421 gatgcggttg ggcaccacgc cgggcgtaac ggggcgctgg atctcgtcgg gcgtgaagac
    24481 gatctcctcg tcgtccgggg cgtcgttcgc gagggctgcg cgatcggagg cgtcatcgac
    24541 cgccgggggg ccgatccggc gcgcgcggcc ggcgcgatag gtttcgcgca acgcatcgaa
    24601 cgggtccgga gcggtcgcgc ggctcgtttg ctccatcatc tccagcagcc ggggggtgtt
    24661 gagcgcgagc tccttctcga gcgcagcgtc atcatccccg atccgtatcc gtttcttggt
    24721 gatccacggc ggttttttcc ccgagcgcgt ttccgtcgca tcggtgtccg gtccggccgt
    24781 tgtttttcgt cgggtgatgc gcgatgcgat cgtcgcaaag gcctgtgcga tgacgcccca
    24841 cgcgcgtatg aatgcgtcgc cggtgcgccg gtcgccctcg gagaggaacg cgagcgagag
    24901 cgcgcctagg aagatgaggc cgatgaggtt caggatgatg acgatgaggt aggccccgac
    24961 cgacccggcc agccgcgtga gcatgaccgc gacatacacg cccaggtagc cgcccgcgaa
    25021 ctgcggcacg ccgctcgtgg agaccaatgc gaacagggcc gacgaggtga tcgccaggta
    25081 cacgagcgcg aagatccgct cgatctcgaa gatcgcgctt ccgcgccgca gcacgctcca
    25141 gccggcgagc gcggtcattg cgaagatgag gtaggcgggg tagccgaacg ccgtgcgcag
    25201 tcccgcgccg acggccgcgc cgaggggacc gaggacgtgc ttgaccgggc ggttctccgc
    25261 cagctgcgca tagtcggtga tgtgatagcc ggccagcgag agcgcgatga tgagcgagag
    25321 catcaggacg agaatgcccg cgatttcctt gtatcggcga tcggtgtgca cgatgccccc
    25381 cggtgtgtca gaacggtcgt gatgacaatg gacatgcgga accgggcccg tcggccgggg
    25441 tccatacgtc ttatatctat atcggctcgc cgtgcggaga tttttagcgg aaagacgggc
    25501 cgatgattga aatggcgacg tcgtctgggt gaccatttgc ggctcgacgc acatattgcc
    25561 cgatgtttgg ggggaggccc cagatgaaat gccagtggat gatgttattt tttttcggag
    25621 atggggcgat gatctgcgct agttgccgcg gtaatcggtg caaaagctat agatgccccg
    25681 tgggatcgat tgcccgtaga ccgccgattt aaagaggaaa acggttcgta tgtcgatgta
    25741 tcgttcgcgt gtaaaaacgg gatagtcctt tttgctgacg acgtatatga tgttgttgcc
    25801 gggagcgcag gggtcgtcgg ttgcgcccgt attgtagcag gcggaattgt aatactttct
    25861 ttttgccgac gcaaggatct tcacgaggat gcatgcgtgc cggtcgggat cgaagcccgt
    25921 ttttttcagt tcccgtgtcg cactgtcgga gaataaaccg taggggagcg tctgtagata
    25981 ggtgatgcgg atttcaccgt ctctcccggc gggcatcccg cttcgatccg caatggcaca
    26041 cgcggccagt gtcgtgatga tgatcgttgc aatacgtgtc aggggggagg atgatcgcgt
    26101 ggttttcatt tcatttttcc gtgcgaatct gctgatccca ttttacgacg gacggcgccc
    26161 gctacacccc gaacacccgc acctcgataa gcgggttctt gtgcagcgac ttgccgatga
    26221 agttcttcac gctcttgcga agcgcgaccg agagatcggc ctccttcatg ccgtcgtcca
    26281 gatagcgctc gcagagcgct tcgacgtcct gcgagatctc ggcggtgatc tccgcgaggc
    26341 gctggctcgt gaaaccgcgc gagacgatgt ccggcgccgc cgcgagccgg ccccgcgaga
    26401 cgaacagggt gatgaagatg atgccgtcgg cggccatcgc ctgccgctcc ttgatgaggc
    26461 tcgagcccat gtcgccgatc tcccggccgt cgaggtacac gtcccggagc tcgatcgcgc
    26521 cggtcttctc gaagctcttc gccgaaagtt cgagcatggc gccgttctcc gcgatgatga
    26581 tgttcgcggg cttgatcgcg agcgattccg cgaggcgtgc gtgcgcgcgc agatgccggt
    26641 actcgccgtg gacgggcatg aaaaatttcg gcttggtcag cgagatcatg agcttgagct
    26701 cctcgcgcga ggcgtgaccg gagacgtgga ggtccgcgtc ggcctcgtag tacacctcgg
    26761 cgccggagcg catgagcgag ttcaccacgt cgtacaccat gcgctcgttg ccggggatca
    26821 cggaggcggt gatcaccacg atgtcgcctt cgcccgcggc caggttcttg tgcgttccgt
    26881 tggcgatgcg cgacagcgcc gacatcggct cgccctgcgt gcccgtgcac agcaccacct
    26941 gccgcttgtg gggcagcgag ttcgcgtttc tgatgtcctc gaagaggtcg tcgtggaagc
    27001 tgaggtagcc cagcgagttc gcgatgtcga tgttcttgag catggtggtg ccggagacga
    27061 gcaccttctt gttgtacttg tgcgccgcgt tcagcacctg ctgtatgcgc cccaggttcg
    27121 aggcgaaggt cgcgatgatg gtgcgcccct tcgcccgcga caggatctcc atgagcttgc
    27181 gctccagcgt gcgctcggaa cgggtgaagc cgtcgcgctc ggcgttggtg ctgtccgaca
    27241 tgagcaacag cacgccgttc tcgccgtact cggcgaagcg gtacatgtcg gtcacctcgc
    27301 cctcgacgga ggtgaggtcc accttgaagt cgcccgtgtg gatgatggtg cccaccggcg
    27361 tggtgatcgc cagccccacg ccgtcgatga tggagtggtt cacccggatg aactcgatca
    27421 cgaaggcgcc gatgtgcacc ttgtcccgcg gcgacatctc gacgaagctc ggggcctcct
    27481 tcggcggctt ctcctccagt cgcttctgga tgaggcccag cgtgagccgc gtcgcgtaga
    27541 ccggcgcatt ggcctcctgc agcagatagg gcatgccgcc gatgtggtcc tcgtgtccgt
    27601 gcgtgatgat gatgccgcgc agccggtctc ggttcttcgc cacgtacgcg aagtcgggaa
    27661 tgaggaaatc gatgccgtgc atgccctcgc tgggaaacat gatgccgcag tccacgacga
    27721 tcatgtcctc gccgtattcg aacacggtca tgttgcgccc gatgccgccg aggcccccga
    27781 ggggaacgat gcggaccttg tcaagttttg ctttcatacg ccggtatgcc caaagccgcc
    27841 cgcgccgcgc gccgtgtccg tgagaacctc cgcggcgacg aacgatgcgc gctccaccgg
    27901 ggcgatcacc atctgcgcga tgcgcatgcc gttctcgatg gagaccggtt cgctccccaa
    27961 gttcgcgaga atgatcttca gctcgccgcg gtagtcggcg tcgatggtgc cgggggtatt
    28021 gagcaacgtg atgccgtggc gtatcgcgag tccgctccgg gggcgtatct gcgcctcata
    28081 gccgtcgggg atctccagga agaggccggt gggcacgagc gtccgtccgc cgggcgcgag
    28141 cacgagcggc gcatcgagcc gcgcgcatac gtcggcgccc gccgccccgg cgctctggta
    28201 cgcgggaagc gtcgcgcccg gatcgagttt gatttttacg tgaaccatga cgctaccggt
    28261 atgcggccgg cgaacggatt tgtcaaggaa atagaggcga tcagcgtccg ggatacagca
    28321 gtgtgaacgc ccagaagacg gccggcagcg tcgccacgca ggcgccgaag ctcgccatga
    28381 ggtactggtt cgcgatggcg cgatttccgc ccagccgctc caccatgatg ggcaccgcgg
    28441 tgatgggcgg caccgccgcc gcgagcgtca tgatgagcgc cacattatag tcgggccgca
    28501 cgagcgcgat cgtccccagc atgcaggcgg ggagcgcgat gtttttacag agcacgaaga
    28561 ccagcgactc gcgcaggtcg aaacggccct tggcggtgaa atcgatgtag atgctgcccc
    28621 cgatgatgat catgataagc gggatcgtca ttgcccccac gctcttggcg atggacacgg
    28681 ccacaccggg tatgtgatcg ccgatgcccc ccaggcgcag cgcgagcgcg atcatcgtcg
    28741 cgacgagcac cggattcagg atgcgcgccc acagggggag tgcgcccgcg gcccggattt
    28801 tccggggata gaacaggtgg tgcgtattga aaaagaaggc gggatacgcg atcgtaaaaa
    28861 gaaaaaggtc aatatgatgc ggcgagctct cgccgaacat gcccgtaaga aacgcgagcg
    28921 gaacaaagag gccgttgtgg tagaagaggg ccatcgcgaa ctcgctccgg tgtttggtgc
    28981 gcgagaggag cgtggcgagg gccgtgaagg cgagcgcgag ggccgtaaag accatccacc
    29041 acagcgggag ctgccaccag ccgggcgcgg cctcgggccg gaagtcgcgg atcataccgg
    29101 cgaagatgag gcagggaagc gcgatgtcga tcaccagcgg cgtgagcacc gtgagcacgc
    29161 tctctggcac catgcgccgg gcgatgacaa aaaagccgat aaggccgata ccgatgggca
    29221 cggccaccga ttcgaacgcg atgctcacgc tattcattgg agggctcctt tcgcaccggc
    29281 aggtgtcgca cgccgcgtgc cgaatgtcga gagaaaaatc cggggtgcgg accgtgccgg
    29341 cttgcgaaat tttcgtttga cgcccgtcgg gcaggggccg tatttaggaa aactgcacct
    29401 cgtatagccg cactaacaag gagatcccgc catgccgaag agaaccgaca tcaacaaggt
    29461 cctgattatc gggtccggac ccatcatcat tggccaggcc tgtgagttcg actactcggg
    29521 cacgcaggcc tgcaaggcgc ttaaaaaact cggctacgaa atcgtcctgg tcaactcgaa
    29581 cccggcgacc atcatgaccg accccggcat ggcggaccgc acctatatcg agccgctcaa
    29641 cgccgagacg atcgagcgca tcatcgaaaa ggagcggccc gacgcgctgc tgcccaacct
    29701 aggcgggcaa acgggcctca acctttcggc ggaactccac aagaatggca cgctcgaaaa
    29761 gtacggggtc cggatcatcg gggtggcggc ggacgcgatc gagcgcggcg aggaccggat
    29821 cgccttcaag gacaccatgg ccgagctcgg actggaggtg ccgcggagcg ctccggccta
    29881 ctcgctcgag gaggcggaga agatcgccgt ggagatcggc tatcccctcg tagtgcgccc
    29941 ggcgtacacg atgggcggaa ccggcagcgg cttcgtctac aataaagagg atctgcgggt
    30001 cgtcgttgcg cgcgggttgc aggagagcct ggtgggacag gtgctcatcg aggagtcggt
    30061 gctgggctgg gaggagctcg agctcgaggt ggtgcgtgac gcgaagaaca acatcgtcac
    30121 cgtctgcttc atcgagaacg tggatgcgat gggcgtgcac accggggact cgtattgcgt
    30181 ggcgcccatg ctcaccatct cgcccgagct gcaggcgcga ctgcaggact actcctatcg
    30241 catcgtcgag gccatcaacg tgatcggcgg cacgaacatc cagttcgccc acaacccgaa
    30301 gaccggccgg gtggtggtca tcgaaatgaa cccccgcacc tcgcgctcct cggcgctggc
    30361 ctcgaaggcg acgggcttcc ccatcgcgct catctcctcc atgctcgccg cggggctcac
    30421 gctggacgag atcccctact ggcgcgacgg cacgttggag aagtacaccc cgtcgggcga
    30481 ttacgtggtg gtcaagttcg cgcgatgggg cttcgagaag ttcgccggcg tcaaggacga
    30541 gctggggacg cagatgcgcg ccgtgggcga ggccatgagc atcggcaaga actataagga
    30601 ggccttccag aaggcgatcc gctcgctcga gaacgggcgc atggggctgg gcttcgcgaa
    30661 ggacttcaac cggaagtcgc tcgacgagct cgtcgcgctg gcgagcaccc ccacgagcga
    30721 gcggcagttc atcatgtacg aggcgctgcg caagggcgcg gacgccgcgc tcctccacga
    30781 gcggacgcac atcaagccct ggtttatcca gcagatgaag gagctcgtcg agctcgaaga
    30841 gcggatcatc gcctgccgcg gcggggagat ccccgacgag ctcctcgcgc aggcgaagcg
    30901 cgacggtttc gcggacgcct acctcgcgaa gatcacgctc gtgttgcgag tgatctatct
    30961 ggacgcgt
//
LOCUS       DAORHF010000013        29538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_937_length_29538_cov_4.737510, whole genome shotgun sequence.
ACCESSION   DAORHF010000013 DAORHF010000000
VERSION     DAORHF010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29538
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_937_length_29538_cov_4.737510"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="PKJ16_02230"
     CDS             <1..560
                     /locus_tag="PKJ16_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV45829.1"
                     /translation="KIVKLQQYYGLSAKFNVIAHSMGGLVTRYYAWQYPYTVAGLVTL
                     GTPHHGSSLANTADFLFLDWFLGAQQAMEHLKPYWVKNTFNKTLAPIMTTPLADGQKL
                     WTIVGDADGWDCWGWGGELFAGWPILGTLEGDNDGLVSHQSAMLSGIDNHNGDANTLA
                     FFWSYDHYDVVRESSVATKAASVLR"
     gene            715..945
                     /locus_tag="PKJ16_02235"
     CDS             715..945
                     /locus_tag="PKJ16_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNV45830.1"
                     /translation="MNTLLHRISVDPAVCFGKPCIKETRIWVSLILDLLAGGMSASQI
                     MDEYPVLSDEDIRAAIAYGAEMSRERFVIIPT"
     gene            949..1317
                     /locus_tag="PKJ16_02240"
     CDS             949..1317
                     /locus_tag="PKJ16_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNV45831.1"
                     /translation="MKFKLDENIGRRAVDVFIRAGHDAHTVYDERLQGCNDQTIYERC
                     CEEQRCLVTLDLDFSNILRFPTERCGGIVIIRTPNNTSLTALEKLIRDFLGMVTADTR
                     INTIWIVEPGRIRIHATDDE"
     gene            complement(1365..2945)
                     /locus_tag="PKJ16_02245"
     CDS             complement(1365..2945)
                     /locus_tag="PKJ16_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV45832.1"
                     /translation="MIRSGRWSRVRAAFQLAAWLFFVALVIALRDPVEREALFGIVPL
                     LSAHLGLATSLAARQLLSAFWPALVVLALTLAFGRFFCGWICPMGATIDATDHAVRPD
                     KINEWRRNGRMLWIKPVKYAVLAVSLILAIAGANAAGIIDPLSLAFRSYSTVLFAYAD
                     RLAKLVFEALAAVPALSAVAEPIYGLLRERALDPNPVVFHGHLAVLAVFVGVLAVSRL
                     ARRFWCQALCPFGAVLALTARIGLLRRVVDTEACTTCRRCERSCRMNAITDSGRGTRE
                     EECVKCFECLDACRDGAISFRFSRPFGKAARPAPAAGGVRTRRQLLAGLASSALLVPL
                     VKHNAEARKDFSAVIRPPGALPEREFLSQCLRCGACMKACPTNALHPAVMEAGLEGLF
                     TPRLIPRIGYCEYSCVLCTRICPTDALRPLAEADKEREVIGTAVIDRNLCIPWAEARE
                     CLVCEEMCPVADKAIKLRRTRMRRNDGKAVSVSLPFVVEDRCIGCGACENKCPVSGSS
                     AITVRVPKKAPASRESSD"
     gene            complement(2950..3912)
                     /locus_tag="PKJ16_02250"
     CDS             complement(2950..3912)
                     /locus_tag="PKJ16_02250"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV45833.1"
                     /translation="MKKVFRREFIRNAFLALGAITLADVIAAIGPLFAKPTTTKRPSN
                     AAGAANTVIVVKGGDLSKATIERMVRTGMAELGGIQRFVKKGMNVVIKPNIGWASKPE
                     RAHNTNPHLVEAVAAMCVKAGARVTIMDRPVHAVDRCYRLSGIQDAAKRCGAKMVAMD
                     KRKFKEVPVKGGLSLSTVEVYEDILNADLVINIPIAKHHGSGTVTLAMKNLMGAIGGS
                     RGKYHIGNLHENIVDFTKAIPTGLVIIDALRVLLDNGPSGGSPKDIAEPRTILFGTNP
                     VTMDAYATRFLNRQPSSVEHIRLAGREGMGVIDPAKMRIVNKAV"
     gene            4190..5548
                     /locus_tag="PKJ16_02255"
     CDS             4190..5548
                     /locus_tag="PKJ16_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45834.1"
                     /translation="MHPACSNMMSSYSEGDVGDGGDAGQAIVAAPCRARPIGLDAPGG
                     IAHGYATFGDPAASPVRCDETPVQIPSDYDTGANGPFAAGTYVRIIENGRNIVDPSGL
                     NLSPVSADTDTPRSPSARLRDDGTRSGECGINAARGWFVLPRPAWWHRLESWDSLSTP
                     VINEYPENAPTTALISEGQSPAAGFYWLISQRGVLPGTFGNAIGAKIVAQSGLASLRR
                     YSTYQWSASREDAIDVFGGAARDLGRGTLSCWIRLRTVESTSSNPYASIANRADASVT
                     IQLSSAPPVRVTFSQTGASACTVSIVIGDATMSSALPHRVDWQHVYIVWDASRSLNAS
                     RAFMLFVNGALHASHANEFPDLSGGTLRIMLAANAMSTVQTFGSDANATLGANDDAGS
                     AGIDNLQIWNHVAAETPVWIYNAGAGRENALHAIYSPENGYRPLLTGAGNGVEYFVPG
                     EP"
     gene            complement(5560..6297)
                     /locus_tag="PKJ16_02260"
     CDS             complement(5560..6297)
                     /locus_tag="PKJ16_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45835.1"
                     /translation="MIHKVMCAVVLSYICVASMGSVASTATAHTVVDYYRQLPATAFG
                     GARYSIRQKKNYWVTTSMADFEIGITVDIPNGYLRIHDEGTGGADLTHELAVFKRADG
                     STVVALSVIDLLASPKMHVTRFFDPGKLWKDVSASVLPKLDPYDLPGSRCIIKQVEAV
                     SAIMRAKGKGALSIAYALPRNGTTVYAGVFVPAVSSLLSVDALVDEEKTSIDEFIDCI
                     GDVRVPLLWDRAKGMFSIGRPVRVKDR"
     gene            6593..9583
                     /locus_tag="PKJ16_02265"
     CDS             6593..9583
                     /locus_tag="PKJ16_02265"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45836.1"
                     /translation="MDLRHWDFARDGSVQLRGEWEMFPGTFLDPKARAAPSTASDAHI
                     IRQPGSWNTQRIQGKPMGPHGFATYRLTLLMPDHAGPLALSYMLAYSAHRLYLDGDLI
                     VRGGEPSPSEDGERREIRPRIATLRPHASRVELVMHVSNHWYYYGGLLEPITVGTQQT
                     IERGLAIESIVHVFLFSIILGMALYHLALFLLRRSDRAPLLFSVFCFASSLHTLIINH
                     AILFFFFPHLCFSDFYTTYHAVFFVTVPAFIAYLRSLYPDEFPASLNLFVLGISGAFM
                     LITIASGIAIVQHLLTAYQALTVALIAYAVVALVRASRRRRFGTRVFIAGFIFLAMGT
                     VNDILVYEDVYSTPFVFPAGLLCFLCSQTYLIAKKFSLAFETSERLAQTLEQRNERLL
                     ELDRLKDEFLAATSHELRTPLNGIIGLAEVLHAKEGLDEKSRNTLSMIVSSGVRLNAL
                     VRDILDFVRIRNRDITLAVVPTDLRAACDTVIELMRPIAAGKRLALENRVPAGLPLVA
                     ADENRLQQVLYNLVGNAMKFTLFGSVSVTAREMTGARGEGLVELSVIDTGIGIPDGDR
                     ERIFEMFEQAAGGIEREHGGAGLGLAIAKRIVELHGGTIGVESKPGAGSRFHFTLPIA
                     APTDAVRPAGRGTAVVSPQELEHAGASTPPRPNPAEQSRVAAAGHILVVDDDLVNCEV
                     ARGFLEQGGFTVSIATRADEAMDRIFVPMERDGARFDLIVLDVMMPHISGLTVCSHIR
                     ARYAPFELPILIFTALGSVGDLVRAFDSGANDYLVKPVRRDELLARSRTLIALKREVQ
                     EHRDARLLLLQNRMKPHFLFNALNTIHALVESGGPGASEAIMTLATIYRFLIDGARQR
                     LVTFDEEWDFTNAYLDFQAIQYGDMLAVSRERSVEFGDMLVPPLIIQPIVENCFKHGF
                     RELAGTGRIALSAELRDGRVRVTVADNGVGPGTEPSFDRSLGNIRDRINHHYGDADVT
                     IGPARDGGTVVGITIRRDSLRGRP"
     gene            9580..10314
                     /locus_tag="PKJ16_02270"
     CDS             9580..10314
                     /locus_tag="PKJ16_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HNV45837.1"
                     /translation="MTATGATYRVLIAEDMAPARSLIRSFVEERPELTCAAVAINGRE
                     VLQRLTAEEFDLALLDISMPLMSGIEAIERAARRPYVIFTTAHAQYAVKAFEIGAIDY
                     LLKPISRERFNAAIDRFLAIAGAAGKRDRAPAADCLSFLESRRQCILPFDEIVYLASH
                     GKKTTIHATARDYEVSALLGDIEARLPTERFVRVHKQHLVNCRYVRELGCDAEGAYTI
                     RLGDADDTALPVGRSFVADAKKSLTR"
     gene            10558..10845
                     /locus_tag="PKJ16_02275"
     CDS             10558..10845
                     /locus_tag="PKJ16_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45838.1"
                     /translation="MEWIYRYLSHFAGFKGIAAASVSLMGQARHRTWKREPRALVMSS
                     LVECPPAGVAIGIKPTTRIGANGLGIAESMTGNMACESHCKTTPVLRHANA"
     gene            10892..12712
                     /locus_tag="PKJ16_02280"
     CDS             10892..12712
                     /locus_tag="PKJ16_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45839.1"
                     /translation="MKSEKNHGVRLYAATFIACVLLLHGCSGGLFDYKDAATGSRTGG
                     DVTNQLTEEQKAYVANLATDFQDSGKIVTDPETGYPSFAAGACEEFDKKLNEKGMRFD
                     CDSQENCDEAKSELDYQLARDQLLQAGIDPYNLQYDSLEDLDRQLEEKFPELLHTTQS
                     AAITNPSPRTASSFSANMRMSSMISNFSIPSDPTQDAHLPSERNELIAVLWCNLIDFI
                     NHPCLSKPQPMFPCCEEKRFRPMPNYKNVIAELRAKLGLREGEPLPQDQEDAMYTKHY
                     QAHIGDINTRYTDIKSWYGRFAVSFGEENLELYKYNGYSPLNEDILKWKPKRILYQVG
                     NEWFDRYFYNGSNNVDLLKKIHSTIRSHIYLYQNRFPDYHPKIYIVGAIPPWFSWGMQ
                     TYDSYYKGVETLNAILEATAREYNATFVDVYGLLHVYDRLHEFLNIDAPVRKFYLTNM
                     GRRIVAYAIIRAIKNAEGVYDSSDPGFIIDNFFIDWKSGEFHPLTPPSGMGTYVYFQL
                     AEHIVESTLTASHDITGSMQTYPIERYTGNGKPREYRIWLSGSIPEDREFHIKVEGFS
                     TKLDQKESFTIQYELSYDPVCIPFTYICTATSSIRGRINY"
     gene            complement(12789..13679)
                     /gene="metF"
                     /locus_tag="PKJ16_02285"
     CDS             complement(12789..13679)
                     /gene="metF"
                     /locus_tag="PKJ16_02285"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HNV45840.1"
                     /translation="MHIGDILTNNKITFSFEFFPPRDPTSSDELFRSIRELVPLKPGY
                     VSVTYGAGGSTRELTHDLVLKIQKETELTVVSHLTCVGSTRDEILEVLTRYKSEGICN
                     IMALRGDPPHGQERFVMTEGGFQFATELVAFIKQHFPGMGIGVAGYPEGHPETPNRLR
                     EMEHLKMKVDAGADYICTQLFFDNRDFYDFRDRCRVAGITVPILAGIMPVTSRKGILR
                     MADLALGARFPARLMKDLARAESDEYVEKVGIHWATTQVDDLIDNGAQGVHFYTLNRS
                     RATREIYASLGASSSEVFNR"
     gene            13896..16046
                     /locus_tag="PKJ16_02290"
     CDS             13896..16046
                     /locus_tag="PKJ16_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV45841.1"
                     /translation="MGHAHHDTTTTAHTAHRNAGKDTRILLTGVFGPFAQNDEYGSRA
                     ENPMELYHNQVTRVQGPFSLRMFHRTFGLLMIEANIEAACTVLEFPTLDRFMEELRDN
                     AYDIVGISAIIPNMLKVKKMCAEIRRLQPGATIVVGGHIANREDLEESIDADHICRGD
                     GIRWFREFLGQDASAPVKHPAVISGFGTRIMGISVPGTRTAAVLIPSVGCPMGCNFCS
                     TSALFGGKGNSVVFYKTGDELFAVLCQLEQELGVGSFFVLDENFLLYRERALRLLELM
                     QRHDKSWAFYVFTSARVLESYTMEQLVGLGVSWVWMGIEGRDSRYSKLNGVDTLELVK
                     RFQANGIRVLGSTIIGLEEHTPENIREAIAHAVAHDTDFHQFMLYTPLPGTPLYHEHR
                     AAGTLLTEDECPAADAHGQTRFNFRHPHIRDGREGEHLIDAFTDDLNTNGPSLARIAR
                     TTLQGWQAHKNHPDPRVVRRYKAGARGLASIYAGSVWAMRHWYRGNRPLVARLTGLLR
                     DLYAEFGLPARIMAPAVGLFVLVNLFREDRRLRSGWTYEPPAIYEKNARAIALESAHP
                     APAKRRTAGIPHPVYDLAWIGQRCREQLEAFQAGLGEQLETAMRELAAIQEQYFREVD
                     SASPCAAKDEHFSRIRERALAGIAQVRERMHDLSDSAQERITHYRSSMTETYRRARHH
                     VDQACDAMHANAELVKLQAQQACARLEREISSVG"
     gene            16142..18397
                     /locus_tag="PKJ16_02295"
     CDS             16142..18397
                     /locus_tag="PKJ16_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HNV45842.1"
                     /translation="MDFIDQLNPNQREAVLHTEGPLLILAGAGSGKTRVITYRIAHLI
                     KNKLVPPYAIFAVTFTNKAAEEMRKRIMTIAGPVGESVFIRTFHAAAVYILRRHGAAI
                     GLPGNFSIFDAKDQAALVKEILLELRIDPKKFTPASLTSKISEIKDKAELYDGADPVA
                     IMPKHYPFSFPNLYAEYHARLAKQNALDFNDLLIRTVALLRESPETLVSLQRRWRYFM
                     VDEYQDTNHAQYLITRALSRATGNLCVVGDDDQSIYSWRGADIRNILEFERDYENARV
                     ITLGENYRSTVPILTAAAAVIQNNVNRKHKALSAQRGDGESAVWCVANNEYGEAEYVV
                     NAIRRHKSTEGVVNADIAIFYRTNAQSRIFEDFLRQENIPYRVVGGLKFYDRKEVKDI
                     MSYLRFICNTRDEVALGRIINTPARGIGKVTLERVRDAAYANNVSEWEIILRAGDYLG
                     KAPEGMARFRELILAAIAAREEVPTSRKLSDFVNDIIERSGYRDDLAAEKSIEARGRL
                     ENLDELLNGVYEYEIRNPDATPDQFLQDISLLTSEENPQEAAAAQAGAVTLMTVHNAK
                     GLEFRVVFLTGMEEGLFPHFNSSDTDEGIEEERRLCYVGITRAMEMLHLTCADVRRGW
                     SGVEYKQPSRFLGEIPEHLLVPVRYGGDSFSGNSFGPQRSFRGGTGRAPQPSRVMDIE
                     APVVPDEPVAFDDEPAFRTRDRVMHPRYGIGTILKIEGTGDNTRLVIDFKSGGKKTFL
                     QKYTPLEKIQR"
     gene            18502..18861
                     /locus_tag="PKJ16_02300"
     CDS             18502..18861
                     /locus_tag="PKJ16_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45843.1"
                     /translation="MDSNYRVSSDMKENTPVLVVEGDITSESDSDIMKTYASIKEKHN
                     PANLIINFEKTNYINSAGIATLINIIQDMGEKNGRVAFVGLSNHFQKVMDIVGISDFV
                     DIVNTNDQAMEKIKARS"
     gene            complement(19145..19429)
                     /locus_tag="PKJ16_02305"
     CDS             complement(19145..19429)
                     /locus_tag="PKJ16_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45844.1"
                     /translation="MFDRVRGHIERWIREQGIYFFDVFCEREESGAGADGFCYTVRVI
                     DRYQRRAHLFRLRFRNGSFELGYLRMSQNPALLRPAGFRMELQQHAGSFC"
     gene            complement(19780..20100)
                     /locus_tag="PKJ16_02310"
     CDS             complement(19780..20100)
                     /locus_tag="PKJ16_02310"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV45845.1"
                     /translation="MARIPHAIEARIQRYLLDLAENNIRIRRAYVFGSYARGTYDEWS
                     DIDIALVSDGFDGDRIADKNKIRRITLRAGSDIEPLPFTLDEFNESNPLVKEILTHGI
                     EIPT"
     gene            complement(20333..20467)
                     /locus_tag="PKJ16_02315"
     CDS             complement(20333..20467)
                     /locus_tag="PKJ16_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45846.1"
                     /translation="MTKDEQVLYWLESARHDLETAETLYANKNSTGACLLGISLLKKL
                     "
     gene            complement(20847..21611)
                     /locus_tag="PKJ16_02320"
     CDS             complement(20847..21611)
                     /locus_tag="PKJ16_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HNV45847.1"
                     /translation="MKKSLIVLCVVAILAAWSSCAKKIEVKEREIGGMKFVWIPSGTY
                     TMGSPEKEEYLADARPQHEVTVDGFWMGKYEVTQKQYHEVLGAKPSLFKGDSNPVETV
                     SWGDAVNFCKRFGTKNKVTARLPTEAEWEYACRAGTTTGFYWGNDYNDAKDYAWYGNH
                     FSERKTYPVGQKKANAYGLHDIIGNVMEWCLDWYAVDYYKNSPRQNPQGPFVGTQRVI
                     RGSSCKGGGGSSYWRYKIVPTFRTEDIGFRVVVAGP"
     gene            complement(21654..22745)
                     /locus_tag="PKJ16_02325"
     CDS             complement(21654..22745)
                     /locus_tag="PKJ16_02325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV45848.1"
                     /translation="MNKNGMRMIYAILVAVLGLLVLSSCASKRSNMLIDPANGDNPQK
                     MLQIAFRSYQDGYYGTAREIASYCIRNNMRVADSCNLLGKCYFAEGDRKQAAVAFKMA
                     IKNNPRLGDAYTGLAIIAAENGEIEESLYHLKAGEPYCQNNAMFHHLSGTLQSSMGNY
                     KNAAGSFLMAISLNPYFGPAYSGLIHCFAKSNQEKEADTVRKLWEKNSAQFAKEMSRK
                     RRDPSWNKQLVEAVIGLDVKTVRDTLHYGADPNQKFNSSPLIVYAAGMGYEDKKGNHW
                     HQRNYDIAKLLLEYGADPNSRCNRGGYALEYAATPSGDSSNLKLVQLLLDHGANINAR
                     DKWGRTILTSLNTSDEDIVKLLIKNGARK"
     gene            complement(22820..23968)
                     /locus_tag="PKJ16_02330"
     CDS             complement(22820..23968)
                     /locus_tag="PKJ16_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45849.1"
                     /translation="MKVKMVCIPLFLGVIFVPMLSHGAFLPEWAQVVAAVGKKTINSV
                     ECWNSRYSGGKTNYDRCMDKARSNYASEVKRFCGSSAPGPGLHLPHKATSVALRCDSI
                     AAQNASRGNFDSSDQARYSVPLGRVSPSVLYNPKQYDVRSVKGGNAAMRKTRPLEITS
                     EETAEYFSAVQSARSPFHDYMVAQQKAASAGDDSSSIFTLDNLALGLQVASALLQGVS
                     DIQAAREQRKSSVSGKSRSSNQASSAGSVPRSIASNPGTRKNSKAYQDWSKSKGGVMN
                     QTPNEIKGKDKAGICGYGSSIRCEGCKLQADYAKAERELSACNCAGTPRHPILFEKMR
                     NIDRRKAAKWDEWFRHTSSCASCRLANLPISDFCDRGPGRHAPCGFGN"
     gene            complement(24037..24288)
                     /locus_tag="PKJ16_02335"
     CDS             complement(24037..24288)
                     /locus_tag="PKJ16_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45850.1"
                     /translation="MNARVRMLLAVTVCAVAVTACNTAAKKKVAEQYDSPDEIRRLTP
                     AKMLDIGFQHYKIGDTGKAIDIAKYCSAALRISYGFRDC"
     gene            complement(24374..25690)
                     /locus_tag="PKJ16_02340"
     CDS             complement(24374..25690)
                     /locus_tag="PKJ16_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45851.1"
                     /translation="MSNKISKSVLIAAVVVIVSFGLYTEAWAWSVTSTLPGAPRCFRL
                     RFRPHATASCKYIEVVNSCSRPVLINGITVKGISNVKSVSEKWWGWGSAQTAGPGKTI
                     DISMVACKESPYVETWDADVILHAEYDTKEEAEIQKQKQKEEEEKREREAEKKRQKEK
                     EREKEREVAQEKERQKQQALAKQQEERARKQKEQEERIRIQNEERKKKQQEQFEREKE
                     RRKQEQQAFEKRKRDEEERRRRDAELRQQQQKDRERAQMEQREKLRQQQEKRKQDQAD
                     AHRKQQEMRKKQWEDQQRAKLEREQKLKEQRDRQQKENYEKTLAKQKAAKELRDKADR
                     DSEARNKKIREDLVKSKRAEMDRKSANRKEQEEAERKREAQARERASNEKKAASNDKT
                     SAGASSWQKSCLNPQRSCIEPAQKSCWMIWRFVSDSWEVIKKLMRL"
     gene            complement(25788..25943)
                     /locus_tag="PKJ16_02345"
     CDS             complement(25788..25943)
                     /locus_tag="PKJ16_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45852.1"
                     /translation="MRYVAMGCDGRVFGTLCVLDDDVIRRGNRFDAILKECKAMIEEH
                     LRMMEMA"
     gene            complement(25972..27156)
                     /locus_tag="PKJ16_02350"
     CDS             complement(25972..27156)
                     /locus_tag="PKJ16_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45853.1"
                     /translation="MMWVKGMAWAVITGSLVFTQVSSLQAGVLDSLAAGINKSADWMQ
                     PDFVKGACSKTKWSDIGGRVACGSKDIVARNMKKFGNFLAGSRSASDGNPILQNDLNR
                     DGVNTRQNSQNLLSATSVTMMSGYLPGSGTRDLDAVADAQEEMRADEELKRQEEAKDR
                     RYIEGCMGTVHEPIQNPFVVTQEDKARAEKDYAAQRAKYEERKSICIAALQNNKEGDK
                     RLAEYLKRKPQQNYDPSKDGKKRRSDLAREEEESRNQARAENPIEKPTDGRADSSPPA
                     DSPGFGNKVSSCMDIAIPVLQVIGYLIPAVANLAESTKRPSVPRQSSAPKVSKAPQQV
                     APAQTAPKKAESPCAWDLGSLSQGGQNKCAPKTKTTAAKKPAARGDEEAGGYNFDTQN
                     MK"
     gene            complement(27209..27847)
                     /locus_tag="PKJ16_02355"
     CDS             complement(27209..27847)
                     /locus_tag="PKJ16_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45854.1"
                     /translation="MRGNKVLNTGFAIVLAVSVAACNTAAKKKIAEQYDSPDKLKKLT
                     PAKMLDIGFQYYKIGDTGKAIDIAKYCIYKNYSRSDAYVLLGKCYYVKKDYRKARAAL
                     EMAGNNGEAYLTLSSVALDEGNVSLALASLKRVPWAGRNNAVYYLNWGDMYNAVGEYK
                     RAAFCYIQAINLNPKFVNAYKRLLHTCKRSRDDALAKRAEELYAVHKIKYGI"
     gene            complement(27986..29494)
                     /locus_tag="PKJ16_02360"
     CDS             complement(27986..29494)
                     /locus_tag="PKJ16_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45855.1"
                     /translation="MNTAVRKIIAVCLAGTACLYASCGTSSSSNVKVKIIKEDLFDAN
                     RLIGGAYRYYQAGDTRKARELLSHCINNKMETGEAFLLMGKCFFAERDYVKASIAFKY
                     AINNSPRRAEGYTSLALIALQQGNVRDGLYILREARPYAAGSAMYHYLNGALNLAMGS
                     GYPAMCAFLKAISINPGFVPAYNGLVECFEKTGNKALVEAVGPLFAELAGGKGSLSAI
                     LALAKKEYEKSLVLCAKSPDGAFLPYDVRKKTPVPTKKDCLSVGIDELPSTLKSLAKK
                     AYFKAAPGVELPSLRGDAIAEDNRGSESFRARSNRPLGVPSEVDVAGRDFLRSSDAII
                     DQLERSQKGAAALADPFYKTKEERKKEPDMWREWQADNKAFVASLDQKVLKSLAKDNR
                     QLSNDSWIDKMLDVAVGVGQAAEALQSGIDKMQQIQGKTGSGAGAQNCCGAMALIDQL
                     GRLPAQETPGQAGKGGKWCQKMNEIQQRARGCADCPQSFKESSQQTFDANCR"
BASE COUNT         5995 a         9305 c         8068 g         6170 t
ORIGIN      
        1 ctaagatcgt caagctccag cagtactatg gcctaagcgc gaagttcaac gtcatcgccc
       61 acagcatggg cggactggtg acccgctact atgcgtggca atatccctac acggtcgcag
      121 ggctcgtaac gctcggcacg ccgcaccacg gttcgtcgct tgcgaacact gcggattttc
      181 tcttcctcga ctggttcctc ggcgcccagc aggcgatgga gcacctgaaa ccgtattggg
      241 tcaagaacac cttcaataag accctcgcgc ccatcatgac gacgccgctg gccgacggac
      301 agaagctctg gaccatcgtg ggcgacgccg acggatggga ctgctgggga tggggcggcg
      361 agctcttcgc cgggtggccg atcctgggca ccctcgaggg cgacaatgac ggcctggtat
      421 cgcaccagtc ggccatgctc tccggtatag acaatcacaa cggcgacgcg aacacgctgg
      481 cattcttctg gagctacgac cactacgatg tcgttcgtga gtcttcggtg gccaccaaag
      541 cggcgtcggt attgcggtaa tcaccgcgca tagcacaccg tcctctttat taacaggaca
      601 ctcaaacggc taaccagccc ccgggaaacc gggggcgttt ttttgacgac gcgcgctttt
      661 tcatttgacg ccccctgcga aggtccatac tttaccatca gggaatacaa tatcatgaat
      721 accctgctac atagaatatc cgtcgatccc gccgtttgtt tcggaaaacc gtgcatcaag
      781 gagacgcgta tctgggtatc gctcattctc gatttactgg ccggcggcat gagcgcctct
      841 caaatcatgg atgaatatcc cgttctctcc gatgaagaca ttcgcgccgc cattgcatat
      901 ggcgcggaaa tgtcccggga gcgctttgtc atcattccca cgtaacgaat gaagttcaaa
      961 ctcgacgaga acatcgggcg aagggcggtt gacgttttca ttcgtgccgg ccatgacgcg
     1021 cacaccgtct acgatgaacg ccttcaggga tgcaacgacc agaccatata cgaacgctgc
     1081 tgcgaagagc aacgctgcct cgtaaccctc gatctcgatt tctccaacat tctcagattc
     1141 ccgaccgaac gctgcggcgg catcgttatc atccgaacgc cgaacaatac gagcctcacg
     1201 gcgctggaga agttgatccg ggactttctg ggcatggtta ctgctgacac gagaatcaac
     1261 accatctgga ttgtagagcc ggggagaatt cggatccatg caaccgacga cgaataatac
     1321 ggctaaccag cccccgggaa accgggggcg tttttttgcg cgcgctaatc cgaggactcc
     1381 cgcgacgcgg gcgccttctt ggggacgcgc acggtgatgg ccgaactccc cgaaaccggg
     1441 cacttgttct cgcaggcgcc gcagccgatg catcgatcct ccaccacgaa cggcaggctc
     1501 accgatactg cctttccgtc gtttcgccgc atgcgcgtcc gcctgagctt gatcgccttg
     1561 tcggctacgg ggcacatctc ctcgcacacc aggcactcgc gcgcctcggc ccagggaatg
     1621 cacaggttcc ggtcgatcac ggccgtaccg atgacctcgc gttccttatc ggcctcggcc
     1681 agcggccgga gcgcgtcggt ggggcagatt cgcgtgcaca gcacgcatga atactcgcaa
     1741 tacccgatgc gcggaatgag acgcggggtg aacaggccct cgagcccggc ctccatgacc
     1801 gccgggtgca gcgcattggt ggggcaggcc ttcatgcaag cgccgcagcg aaggcactgg
     1861 gagagaaatt cgcgctccgg aagcgccccc ggcgggcgga tgacggccga gaagtccttt
     1921 cgcgcctcgg cattgtgctt caccagcgga acgaggaggg ccgacgaggc cagcccggcc
     1981 aggagctgcc gacgcgtgcg aaccccgcct gccgcaggcg ctggccgcgc cgccttcccg
     2041 aacggccgcg agaaccggaa ggagatcgcc ccatcgcgac aggcgtccag gcactcgaag
     2101 catttgacgc actcctcctc gcgcgtgccg cgacccgagt cggtgatggc gttcatgcgg
     2161 caggagcgct cgcagcgccg gcatgtcgtg cacgcctccg tgtccacgac gcgtcgaagc
     2221 aggccaatgc gcgcggtgag cgcgagcacg gcgccgaagg ggcacagcgc ctgacaccag
     2281 aaccggcgcg cgaggcgcga gacggcgagc acgcccacga aaactgcaag cacggcgaga
     2341 tggccgtgaa ataccacggg attgggatcg agcgcgcgct ccctgagaag gccgtatatc
     2401 ggctcggcaa ccgccgagag cgccggaacg gcggcgagcg cctcgaagac gagcttggcc
     2461 agccgatcgg cgtaggcgaa cagcacggtc gaatagctcc gaaaggcgag cgacagcgga
     2521 tcgatgatac cggccgcgtt cgcgccggcg atcgcgagga tcagcgatac ggcgagcacc
     2581 gcatacttga ccggtttgat ccagagcatg cggccgtttc gccgccactc gttgatttta
     2641 tcgggccgca cggcatggtc cgtcgcgtct atcgtcgcgc ccatgggaca gatccatccg
     2701 cagaagaacc ggccgaacgc aagcgtgagg gcaaggacca caagcgcggg ccagaacgcc
     2761 gagaggagct gtcgcgcggc aagcgaggtc gcgagaccca ggtgcgccga caaaaggggc
     2821 acgatcccga agagcgcctc gcgctcgacc ggatcgcgca gtgcgataac gagcgcgacg
     2881 aagaagagcc acgcagcgag ctgaaacgcc gcccgcacgc gcgaccagcg gccgctgcgg
     2941 atcataccgc tacaccgcct tgtttacaat gcgcattttg gccggatcga ttactcccat
     3001 gccctcgcgc cccgcgagcc ggatatgctc aacggacgac ggctggcggt tgaggaagcg
     3061 cgtggcgtag gcgtccatcg tgacgggatt ggtgccaaac aggatcgtcc ggggctcggc
     3121 gatgtccttc ggggagccgc cggaggggcc gttgtccagg agcacgcgca gggcgtcgat
     3181 gatcaccagg cccgtcggga tcgccttggt gaaatcgacg atgttctcgt gcaggttgcc
     3241 gatgtgatac ttgccgcgac tcccgccgat cgcgcccatg aggttcttca tggcgagggt
     3301 caccgtcccc gagccgtggt gcttggcgat ggggatgttg atgacgagat cggcgttgag
     3361 aatgtcctcg tacacctcga ccgtcgagag gctgaggccg cccttgacgg gcacctcctt
     3421 gaacttccgc ttgtccattg cgaccatctt cgcgccgcaa cgcttcgcgg cgtcctggat
     3481 gccgctcagt cgatagcagc ggtccaccgc atgcaccggg cggtccatga ttgtcacgcg
     3541 cgcgcccgcc ttcacgcaca tcgccgcgac ggcctcgacg agatggggat tggtattgtg
     3601 cgcgcgttcg ggcttggagg cccatccgat attgggcttg atgactacgt tcatgccctt
     3661 cttcacgaag cgctggatgc cgccgagctc ggccatgccg gtccgcacca tccgctcgat
     3721 ggtggccttc gaaagatcgc cgcccttcac gacaatgacg gtgttcgcgg cccccgcggc
     3781 gttcgaaggg cgtttcgtcg ttgtcggttt cgcgaacagc gggccaatcg cagcgataac
     3841 atcggccagg gtgattgcgc caagggcaag aaacgcgttt ctaatgaatt cccgtctgaa
     3901 cacctttttc atgagaacct ccggcacatg cagaatcctc acatgatagc gcacatcgag
     3961 gaaaatacaa tcaaatagtt ggcctcattc ggtttgaacc ttgcgaccat accgcttaaa
     4021 aaaagcttga tatttagcca ctatgggaat actgttgact aattgaatca taacgagaca
     4081 catgaaatat aaattcgctt ttcctgttcg acagcaaagg ccccgctgga cgcgaatgat
     4141 ctcattctca atacataaag cgctcttgcc gctcgtggta ctcgcatgca tgcaccccgc
     4201 ctgctcgaat atgatgtcga gctattccga aggcgacgtt ggcgacggag gggatgcggg
     4261 tcaggcgatc gtagccgcgc catgccgggc gcgtccgata gggctcgatg cgccgggggg
     4321 gatcgcacat gggtacgcga catttggcga tccggccgca tcgccggtcc gatgcgatga
     4381 aacccccgtc cagatcccgt ccgattacga cacgggcgcc aacggccctt tcgccgccgg
     4441 cacctatgtg cgaatcatcg aaaacggccg taatatcgtc gatccgagcg gcctcaatct
     4501 ttcgccggtc tcggcggaca ccgatacgcc gcgctcgcca tccgcgcgat tgcgcgatga
     4561 cggcacccgt tcgggcgagt gcggtatcaa tgcggcgcgg ggctggttcg tgctcccccg
     4621 tcccgcatgg tggcaccggc tcgagtcatg ggactccctc tcgacaccgg tgattaacga
     4681 gtatcccgaa aacgcgccaa ccaccgcgct catctccgaa ggccagtccc ccgccgcggg
     4741 tttttactgg ctcatctcac agcggggcgt cctgccggga acattcggaa acgcgatcgg
     4801 cgcgaagatc gtcgcgcagt ccgggctagc atcgctcagg cgctacagca cctaccaatg
     4861 gagcgcgagc cgcgaagacg ccattgacgt attcggaggc gccgcacgtg acctcggccg
     4921 cggaacgctg tcgtgctgga tcaggcttcg aacggtggag agcacctcct cgaatcccta
     4981 cgcgtccatc gccaaccggg ccgacgcgtc cgtgacgatc cagctgtcgt cggcgccgcc
     5041 ggttcgcgtg accttctcgc agaccggcgc aagcgcctgc acggtgagta tcgtcatcgg
     5101 cgatgcgacg atgtcctcgg ccctgcccca tcgcgtcgat tggcaacacg tctatatcgt
     5161 ctgggacgcc tcgcgctcgc tcaatgcgtc gagggcattc atgcttttcg tgaacggcgc
     5221 cctccacgcc agccatgcga acgaattccc ggacctgtcc ggcgggacac tgcgcatcat
     5281 gctcgcggcg aacgccatga gcacggtgca aaccttcgga agcgatgcga acgcgacact
     5341 gggcgccaac gacgacgccg gatccgccgg gatcgacaat ctccagatct ggaaccacgt
     5401 cgcggcggaa acccccgtat ggatttacaa tgccggcgcg ggcagggaga acgcgttgca
     5461 cgcaatctat tcccccgaaa acggttaccg cccgctcctc accggggctg gaaacggggt
     5521 tgaatatttt gttcccgggg aaccctgacg cgcggagcgt cagcggtcct tcacccgtac
     5581 gggcctgccg atcgaaaaca tccccttcgc acggtcccag agaagcggga cgcgcacatc
     5641 accgatacag tcgataaact cgtcgatcga cgtcttctcc tcgtcgacaa gcgcatcgac
     5701 ggacaacagg gacgaaacgg ccgggacgaa aacgccggca tagacggtcg tgccgttgcg
     5761 cggaagggcg tacgcaatgg aaagggcgcc ctttcccttc gcgcgcatga tcgcggagac
     5821 ggcttccact tgcttgatga tgcagcgcga gccggggaga tcgtacggat cgagcttcgg
     5881 cagcaccgat gcgctcacgt ctttccataa tttcccggga tcgaagaagc gggtgacgtg
     5941 cattttcggc gaagcgagga gatcgatgac ggaaagcgcg acgaccgtcg atccgtcggc
     6001 ccgtttgaat acggccagct cgtgcgtcag gtcggcgccg ccggttccct cgtcgtggat
     6061 gcgcaggtac ccattgggga tgtcgacggt gatcccgatc tcgaaatccg ccatcgaggt
     6121 cgtcacccag taatttttct tttgccgtat cgaatacctg gcgccaccga atgcggtcgc
     6181 cggcagctga cgatagtagt cgaccacggt gtgcgcagtc gcggtcgagg cgaccgaacc
     6241 catcgatgcg acacagatgt acgacagaac gacggcacac atgaccttat gaatcatcgc
     6301 gaagcacctc ctcgaacagg cattcgaatc gctgaaggta cggaatcata tcacgagcac
     6361 tcctgcaagg tttttttacg cgccgtacaa ggcccccccg ggcggaaacc cgccgaagaa
     6421 taccataaaa aagttgccgc acaaggctcc gatggcatat catgccgcct gaaacgcacc
     6481 cattgacatg acagcgcgct cccgaaacct ttttcccgcg atatgcctcg ccctgatcac
     6541 gacatggtgc gccggatgcg tccgcgatgt ccccgtcgca aaacggggcg tcatggacct
     6601 tcgccactgg gacttcgccc gcgacgggtc cgtgcagctt cggggcgaat gggagatgtt
     6661 tccgggcaca ttcctcgacc caaaggcaag agcggcccca tcgactgcat ctgacgcgca
     6721 tatcatccgg cagcccggat catggaacac ccagcgcatc caaggaaaac cgatgggccc
     6781 gcatggattc gcgacctatc ggctcacgct gctcatgccc gatcacgccg ggccgctcgc
     6841 cttgtcctat atgctcgcct actcggcgca tcggctctat ctcgacggcg atctcatcgt
     6901 ccggggcggg gagccgtccc cgagcgaaga cggcgagcgc cgcgaaatcc ggccgcgcat
     6961 cgcgacgttg cgcccgcacg cctcgcgcgt cgagctcgtg atgcacgtct cgaaccactg
     7021 gtactattac ggagggctcc tcgagccgat caccgtgggc acgcaacaga ccatcgagcg
     7081 cgggcttgcc atcgaatcca tcgtacacgt gttcctgttc tcgattatcc tcgggatggc
     7141 gttgtaccac ctcgccctgt ttctcctgcg gcgatcggac cgcgcgcccc tgttgttctc
     7201 ggtgttctgc ttcgcatcgt cgctgcacac gctcatcata aatcacgcga tattgttttt
     7261 ctttttcccg cacctgtgtt tttccgactt ttacacgacc taccacgccg tgttcttcgt
     7321 cacggtgccc gcgttcatcg cgtacctgcg ctcgctctac ccggacgaat ttcccgcatc
     7381 gctcaacctg ttcgtgctgg ggatcagcgg cgcatttatg ctgatcacga tcgcgagcgg
     7441 catcgcgatc gtccagcacc tgctcacggc ataccaggcg ctgacggtcg cgctgatcgc
     7501 ctacgccgtc gtcgccctcg tccgcgcgtc ccggcggcgc cggttcggga cacgggtgtt
     7561 catcgccggt ttcatctttc tggccatggg caccgtgaac gacatcctcg tttacgagga
     7621 cgtgtattcg accccgttcg tgtttcccgc ggggctcctg tgcttcctgt gctcccagac
     7681 ctatctcatc gcgaaaaagt tttccctggc cttcgagacc tccgagcgtc tcgcgcaaac
     7741 cctggagcag agaaacgaac gcctcctcga gctcgaccgg ctcaaggacg agttcctggc
     7801 cgcgacctcc cacgagctcc ggacgccgct caatgggatc atcgggcttg cggaggtcct
     7861 tcacgcgaag gagggcctcg acgagaaatc gcgcaacacg ctctcgatga tcgtctcgag
     7921 cggcgtgcgc ctcaatgcgc tcgtacgcga cattctcgat ttcgtacgca tccggaaccg
     7981 cgacatcacg ctcgcggtcg tgccgacgga cctccgcgca gcgtgcgaca cggtcatcga
     8041 gctgatgcgc ccgatcgccg ccggcaagcg cctggcgctc gagaaccgcg tcccggccgg
     8101 cctccccctc gtcgcggccg acgagaaccg attgcagcag gtcctgtaca atcttgtcgg
     8161 caacgcgatg aagttcacgc tgttcggatc ggtgtctgtg accgcgcgcg aaatgaccgg
     8221 cgcgcgcggc gaggggctgg tcgagctttc ggtcatcgac accggcatcg gcataccgga
     8281 cggcgaccgc gagcggatct tcgagatgtt cgaacaggcg gcgggcggga tcgagcgcga
     8341 gcacgggggc gcgggactgg gactggccat cgccaagcgg atagtcgagc tgcatggcgg
     8401 gaccatcggc gtggaatcga aaccgggcgc gggatcgcgc ttccatttca cgctgccgat
     8461 cgccgcaccg acggacgctg tgcggcccgc gggtcgcggg accgctgtcg tctcaccgca
     8521 ggaactcgag cacgcaggcg catcaacacc cccgcgcccg aaccccgcgg agcaatcgcg
     8581 cgtcgccgcc gcgggccaca tcctggtggt ggacgacgac ctcgtcaact gcgaggtcgc
     8641 gcggggtttt ctcgaacagg ggggcttcac ggtgtccatc gccacgcgcg ccgatgaagc
     8701 gatggaccgc atcttcgtcc cgatggagcg cgacggggcc cggttcgacc tcatcgtgct
     8761 cgacgtcatg atgccgcaca tctccggcct caccgtgtgc tcgcacattc gcgcgcggta
     8821 tgcgcccttc gagctgccca ttctcatctt cacggcccta ggctccgtgg gcgacctggt
     8881 ccgcgccttc gacagcgggg ccaacgacta cctcgtgaag ccggtgcgcc gcgacgagct
     8941 gctggcgcgc tcgcgaacgc tcatcgcgct caagcgcgag gtgcaggagc accgtgacgc
     9001 gcggctcctc ctgctccaga accgcatgaa gccgcacttc ctgttcaacg cgctcaatac
     9061 catccatgcg ctcgtcgagt ccggcgggcc cggcgcgagc gaggcgatca tgacgctcgc
     9121 gaccatctat cgattcctga tcgacggggc gaggcagcgg ctggtcacct tcgacgagga
     9181 atgggacttc acgaacgcgt atctcgattt ccaggccatc cagtacggcg acatgctcgc
     9241 ggtgtcccgc gagcgctccg ttgaattcgg cgatatgctg gtcccgcccc tcatcatcca
     9301 gcccatcgtg gaaaactgtt tcaagcacgg cttccgggag ctcgcgggga ccggccgcat
     9361 cgcgctttcg gccgaactgc gcgacggacg cgtccgcgtg acggtcgccg acaacggcgt
     9421 cggcccgggg accgaaccgt cgttcgaccg gtcgctcggg aacatccgcg acaggatcaa
     9481 ccaccattac ggggatgccg atgttaccat tggacccgcg cgcgacggcg gcaccgtcgt
     9541 cggaatcacc atccgcagag attcattgag ggggcgccca tgaccgccac aggtgcgaca
     9601 taccgcgtgc ttatcgccga ggacatggcc ccggcccgat ccctcatccg ctctttcgtc
     9661 gaggagcgtc ccgagctgac ctgcgcggcc gtcgccatca acggccggga ggtgttgcag
     9721 cgcctcaccg ccgaggagtt cgatctggcc ctgctggaca tcagcatgcc cctcatgtcc
     9781 gggatcgagg cgatcgagcg ggccgcgcgg cgcccctacg tcatcttcac cacggcgcac
     9841 gcgcagtacg cggtcaaggc cttcgagatc ggcgcgatcg actatctgct caaaccgatc
     9901 tcccgcgagc ggttcaacgc ggcgatcgac cgctttctcg cgatcgcagg cgcggccggg
     9961 aagcgggacc gcgcgccggc ggcggactgc ctctcgttcc tcgagagccg gcgacagtgc
    10021 atccttccct tcgacgagat cgtctacctg gcctcgcacg gaaagaagac gacgatccac
    10081 gcgaccgcgc gcgattacga ggtctccgcg ctcctgggcg acatcgaggc ccggctcccg
    10141 accgaacgct tcgtgcgcgt ccacaaacag cacctggtga attgccgtta tgtgcgcgag
    10201 ctcggctgcg acgccgaggg cgcctacacc atccggctcg gcgacgcgga cgacacggcg
    10261 ctgcccgtgg gacggtcgtt cgtcgccgac gcgaagaagt ccctcacgcg ctgacgtcgc
    10321 tctccgaaca cgcggagaac caccaatcgc gcctagctcc catcacgacg cgcatcaacc
    10381 cagccgccat gcgatcccct tcggcatgtc gttcattccg caagacctga cgttcattct
    10441 ccttcccggg cgcgcgtcgt ctcccgcatt gacgcacccg cgccatgtct ggtactgtcc
    10501 cgccatacac gcattgacgc acctgcatgc atgaaaaacg caggggagag aacggacatg
    10561 gaatggatct atcgctatct gtcgcacttc gcaggcttca aaggaatcgc cgccgcaagc
    10621 gtatcgctga tgggccaggc gcgccatcgg acgtggaagc gggagccacg cgcgctcgtc
    10681 atgtcgtcgc tggtcgaatg cccaccggcc ggcgtggcca tcggcataaa gccgacaaca
    10741 cgcatcgggg ccaacggtct tggcatcgcc gaaagcatga cgggaaacat ggcatgcgaa
    10801 tcacactgca aaaccacgcc cgttctgcgg catgccaatg cataacctgg aacaagcaag
    10861 aaaagcaact ccatatcaca ggggggtcac aatgaaatcc gaaaaaaacc atggcgtccg
    10921 attatacgcc gcaacgttca tcgcatgcgt cctgttgctg cacggctgca gcggcgggtt
    10981 attcgactac aaggacgcgg caacaggttc ccggactggc ggcgatgtca caaatcagtt
    11041 aaccgaagag cagaaggcat acgtggccaa tctggcgacc gatttccagg acagcggaaa
    11101 gatagtaacg gacccggaaa cggggtatcc ttcattcgcc gcgggcgcat gcgaagaatt
    11161 cgacaagaag ctcaacgaga agggcatgcg attcgattgc gacagccagg aaaactgcga
    11221 tgaggcaaaa agcgagcttg actaccaact ggcgcgcgac cagctccttc aagcgggaat
    11281 cgatccatac aacctccaat acgattcact tgaggatctc gacaggcaac tcgaggaaaa
    11341 attcccggaa ttgctacata cgacgcagtc cgccgccatc accaatccat caccgcgtac
    11401 cgcatcgtcg ttttccgcaa acatgcgtat gtcgtcaatg atctcaaact tcagcatccc
    11461 gtctgatccc acccaagatg cgcatctgcc cagcgaaaga aatgagctga tagcggttct
    11521 ctggtgcaac ttgattgatt tcatcaacca cccatgcctg tcgaaacccc aaccgatgtt
    11581 cccgtgctgc gaggaaaaac ggttccgccc catgccgaac tacaagaacg tgatcgcaga
    11641 acttcgcgca aagctcggtc ttcgcgaggg cgaaccgctg ccccaggacc aggaagacgc
    11701 aatgtatact aaacattatc aagcgcatat tggggacatc aatactcgct ataccgacat
    11761 caaaagctgg tatggaaggt tcgctgtcag tttcggcgaa gagaatctcg agctttacaa
    11821 gtacaacggg tattcgccac taaacgagga tatactcaaa tggaaaccga agaggatact
    11881 atatcaggtg gggaatgagt ggttcgaccg ctatttctac aatggcagca acaacgtcga
    11941 ccttctcaaa aaaatacact caacgatacg aagccacata tacctctatc aaaatcgatt
    12001 cccggactac cacccaaaga tttacatagt aggcgccatt ccgccgtggt tttcctgggg
    12061 catgcaaacg tatgattcat actacaaagg cgtagagacg ctcaatgcca tactggaggc
    12121 gacggcgaga gaatacaacg ccacattcgt ggacgtgtat gggctcctcc acgtttacga
    12181 ccgtcttcat gaatttttga acatcgacgc gcccgtccgg aaattttacc tgacaaacat
    12241 gggcagaaga atcgtcgcct atgcgattat cagggcgatt aaaaatgcgg agggtgtcta
    12301 tgacagctcc gatccgggtt tcatcattga taatttcttc atcgactgga aaagcggaga
    12361 gttccacccc ctgaccccgc caagcggaat gggcacgtat gtctactttc aattagcgga
    12421 acatatcgtc gagagcactc tcacggcatc ccacgatatc accggctcca tgcagacata
    12481 cccgattgaa cgctacaccg gcaacggaaa gccgcgggaa taccggatat ggctgagcgg
    12541 ctcgataccc gaggatcggg aatttcacat taaagtcgag gggttcagca caaaacttga
    12601 ccagaaggag agcttcacca tccagtatga actttcatac gatccggttt gcatcccatt
    12661 tacttatata tgcacggcca catcttccat tcggggaaga atcaattatt gatcacacgc
    12721 acatgccgca ccatcacaca gcctataaaa aacgcatccc ttcggggatg cgttttttac
    12781 gaccgcacct accggttgaa cacctccgac gagctcgcgc ccaacgacgc atagatctcg
    12841 cgcgttgcgc gcgatcggtt gagcgtgtag aagtggaccc cctgcgcgcc attgtcgatg
    12901 aggtcgtcca cctgggtggt ggcccaatgg atgcccacct tctcgacgta ctcgtcgctc
    12961 tcggcgcgcg cgaggtcctt catgaggcgc gcggggaagc gcgcgcccag cgcgaggtcg
    13021 gccatgcgga ggatcccctt gcgggaggtg accggcatga tgccggcgag gatggggacg
    13081 gtgatcccgg cgacgcggca gcggtcgcgg aaatcgtaga aatcgcgatt atcgaagaag
    13141 agctgggtgc agatgtaatc cgcgccggca tcgaccttca tcttgaggtg ctccatctcg
    13201 cgcagccgat tgggcgtctc cgggtgcccc tccggatagc ccgcgacgcc gatccccatg
    13261 ccggggaaat gctgtttaat aaatgcgaca agctccgtcg cgaactgaaa gccgccctcg
    13321 gtcatcacga accgctcctg cccatggggc ggatcaccgc gcagcgccat gatattgcag
    13381 attccctcgc ttttatagcg cgtgaggacc tccagtatct catcgcgcgt cgagcccacg
    13441 caggtaagat gcgacacgac ggtcaactcg gtctctttct ggatcttcag cacgaggtcg
    13501 tgcgtgagct cgcgcgtcga tccgcccgcc ccgtaggtga cgctcacata gccgggcttg
    13561 agcggcacca gctcccggat cgagcgaaag agctcatcag atgatgtggg gtctcgcggc
    13621 ggaaaaaact cgaagctaaa ggtgatcttg ttgttcgtga ggatatcgcc gatatgcatg
    13681 tgcctccact ctcccgcgca ggtagcgcat cgatcgctcg cacgtttcac ccgcgctgtc
    13741 aatcaccttt tccccggcgc ggccgtccgg gcggattccc tccccgcttt gcctcttgac
    13801 agaaatgggg cgcggtgaga gaatggagcc cgatatactg ggcgggtgat tggcccatcg
    13861 cggcccgctg aaaacttcaa ggacacgcac gaaccatggg acacgcacac cacgacacca
    13921 caacgaccgc gcatacagcg caccgcaacg ccgggaagga cacccgcatc ctgctcacgg
    13981 gcgtcttcgg ccccttcgcg cagaacgacg aatacggcag ccgcgccgag aaccccatgg
    14041 agctctacca caaccaggtg acgcgcgtac agggcccctt ctcgctgcgc atgttccacc
    14101 gcaccttcgg gctgctcatg atcgaggcga acatcgaggc cgcctgcacg gtgctggagt
    14161 tccccacgct cgatcgcttt atggaagagc tgcgcgacaa cgcctacgac atcgtgggca
    14221 tcagcgccat catccccaat atgctgaaag tgaaaaagat gtgcgcggag atccgccgcc
    14281 tccagcccgg ggccaccatc gtggtcggcg gccatatcgc caaccgcgag gacctcgagg
    14341 agagcatcga cgccgaccac atctgccgcg gcgacgggat ccgctggttc cgcgagtttc
    14401 tgggccagga cgcgagcgcc cccgtgaagc acccggcggt catctcgggg ttcggcacgc
    14461 gcatcatggg catcagcgta cccggcacgc gtacggcggc cgtgctgatc ccgtcggtgg
    14521 gctgccccat gggctgcaac ttctgctcca cctcggcgct cttcggcggc aagggaaact
    14581 ccgtcgtctt ctataaaacc ggcgacgagc tcttcgccgt gctctgccag ctcgagcagg
    14641 agctcggggt cggctcgttc ttcgtgctcg acgagaactt cctcttgtac cgcgagcgcg
    14701 cgctgcgcct gctcgagctc atgcagcggc acgacaagag ctgggccttc tacgtcttca
    14761 cctccgcgcg cgtgctcgaa tcgtacacca tggagcagct cgtgggactc ggtgtctcgt
    14821 gggtgtggat gggcatcgag ggccgcgaca gccgttattc gaaactcaat ggcgtggaca
    14881 ccctggagct ggtgaagcgc ttccaggcga acggcatccg cgtgctcggc tcgaccatca
    14941 tcggcctcga ggagcacacc ccggagaaca tccgcgaggc catcgcccac gccgtggccc
    15001 acgacaccga cttccaccag ttcatgctct acaccccgct ccccggcacg ccgctgtatc
    15061 atgagcaccg cgcggcgggc acgctgctca ctgaggacga gtgccccgcg gccgacgcgc
    15121 acggccagac gcgcttcaac ttccggcatc cgcacatccg cgacgggcgc gagggcgagc
    15181 acctcatcga cgcattcacc gacgacctga acacgaacgg ccccagcctc gcgcgcatcg
    15241 cccgcacgac gctccagggc tggcaggcgc acaagaacca tccggacccg cgcgtcgttc
    15301 gccgatacaa ggcgggcgcc cggggcctcg cgagcatcta tgcggggtcc gtgtgggcca
    15361 tgcgccactg gtaccgcggc aaccggcctc tcgtcgcgcg cctcacgggc ctcctgcgcg
    15421 acctctacgc cgagttcggc cttccggcgc gcatcatggc gccggccgtg gggctgtttg
    15481 tgctcgtgaa cctttttcgg gaggaccgcc gcctcaggag cggctggacc tacgagcccc
    15541 cggccattta cgagaagaac gcccgcgcga tcgcgctcga gagcgcgcac ccggctccgg
    15601 cgaaacgccg aaccgccggc ataccgcacc cggtgtacga cctcgcgtgg atcggccagc
    15661 gctgccgcga gcagctcgag gccttccagg cgggcctggg cgagcagctc gagacggcga
    15721 tgcgggagct tgccgcgatc caggagcagt atttccggga ggtcgattcg gcctcgccgt
    15781 gcgccgcgaa ggacgagcac ttcagccgga ttcgcgagcg cgcgcttgcg ggcatcgcgc
    15841 aggtgcgcga gcgcatgcac gacctgtccg attcggccca ggagcggatc acgcactacc
    15901 gttcgtcgat gaccgagacc taccgccgcg cgcgccatca cgtggaccag gcctgcgacg
    15961 cgatgcacgc gaacgccgag ctcgtgaagc tccaggcgca gcaggcctgc gcgcgcctgg
    16021 agcgggagat ctcctccgtc ggatgatcgc gccgtcgcgc cgtcgggcct tccgcacggc
    16081 gcgacgcatg ccgtgaaaaa actgcttgtc actgcggaac gatgacacta agaggttccg
    16141 catggacttc atcgaccagc tcaatcccaa ccagcgcgag gccgtactac acaccgaggg
    16201 accgctcctc atcctcgcgg gggcgggctc cgggaagacc cgcgtgatca cctaccgcat
    16261 cgcgcacctc atcaagaaca agctggtgcc gccctacgcg atcttcgccg tgaccttcac
    16321 caacaaggcg gccgaggaga tgcgaaagcg catcatgacg atcgcgggcc cggtgggaga
    16381 gagcgtgttc attcgcacct tccacgcggc cgcggtgtac atcctgcgcc gtcacggcgc
    16441 tgcaatcggc ctgccgggga acttctcgat cttcgacgcg aaggaccagg ccgcgctggt
    16501 caaggagatc ctcctcgagc tgcgcatcga cccgaagaag ttcacgccgg cctcgctcac
    16561 ctcgaagata tccgagatca aggacaaggc cgagctctac gacggcgcgg accccgtcgc
    16621 gatcatgccc aagcactacc ccttcagctt cccgaacctc tacgccgaat accatgcgcg
    16681 cctggccaag cagaacgcgc tcgacttcaa cgacctcctc atccgcacgg tcgcgctgct
    16741 gcgcgaaagc cccgagaccc tcgtctcgct ccagcggcgc tggcgctact tcatggtgga
    16801 cgagtaccag gacaccaatc acgcgcagta cctcatcacg cgcgccctct cgcgcgcaac
    16861 aggcaacctc tgcgtggtgg gcgacgacga ccagtcgatc tactcgtggc ggggcgccga
    16921 catccgcaac atcctcgagt tcgagcgcga ctacgagaac gcccgcgtca tcacgctcgg
    16981 cgagaactac cgctccaccg tccccatcct caccgccgcg gccgcggtga tccagaacaa
    17041 cgtgaaccgc aagcacaagg cgctcagcgc gcagcggggc gacggcgaga gcgccgtctg
    17101 gtgcgtcgcg aacaacgagt acggcgaggc ggaatacgtg gtgaacgcca tacgccgcca
    17161 caagtcgacc gagggggtcg tgaacgcgga catcgcgatc ttctaccgga ccaatgcgca
    17221 gtcgcggatc ttcgaggact tcctgcgcca ggagaacatc ccctaccgtg tggtgggcgg
    17281 cctcaagttc tacgaccgca aagaggtcaa ggatatcatg tcgtacctgc gcttcatctg
    17341 caacacccgc gacgaggtgg ccctggggcg catcatcaac acgccggcgc gggggatcgg
    17401 caaggtcacc ctcgagcgcg tgcgcgacgc cgcgtacgcc aacaacgtct ccgaatggga
    17461 gatcatcctg cgggcgggag actatctcgg caaggccccc gagggcatgg cccgctttcg
    17521 cgagctcatc ctcgccgcca tcgccgcgcg cgaggaggtc ccgacatcgc gaaagctttc
    17581 cgatttcgtc aacgacatca tcgagcgctc ggggtatcgc gacgatcttg cggcggaaaa
    17641 atccatcgag gcccgcggcc gcctcgagaa cctggacgag ctcctcaacg gcgtttacga
    17701 gtacgagatc cgcaatcccg acgccacgcc ggaccaattc ctacaggata tatccctcct
    17761 cacctccgag gagaaccccc aggaggccgc ggccgcgcag gcgggcgccg tcacgctcat
    17821 gacggtgcac aatgcgaagg ggctcgagtt ccgcgtggtc ttcctcaccg ggatggagga
    17881 gggactgttc ccgcacttca attcaagcga caccgacgag gggatcgagg aggagcggcg
    17941 cctctgctac gtgggcatca cgcgcgccat ggagatgctc cacctcacct gcgccgacgt
    18001 ccgacgcggc tggagcggcg tggaatacaa gcagccgtcg cgctttctcg gggagatccc
    18061 cgaacacctc ctggtcccgg tccgctatgg gggcgactcc ttttccggca attcgtttgg
    18121 cccccaacgg agctttcgcg ggggcacggg acgcgcgcca caaccctcgc gggttatgga
    18181 catcgaagct ccggtcgttc ccgatgaacc ggtcgccttt gacgacgagc ccgcatttcg
    18241 cacccgggac cgcgtgatgc acccccgcta tggaatcggg acgatcctca agatcgaggg
    18301 caccggcgac aatacccgtc tcgtgattga cttcaaatcg ggtggaaaaa aaacattttt
    18361 gcagaagtat acacctcttg agaagataca acggtaaaat aatgcccagc cgggccaaaa
    18421 aatgttgatt tttttcatca ttgattttag cgtgctgcaa agatgcatcg aacgggaaac
    18481 atcatagacg gggaaggaac catggacagc aactaccggg tatcgtctga catgaaggaa
    18541 aatacccccg ttctcgttgt cgaaggagat atcacctccg aatcggacag cgacatcatg
    18601 aaaacatacg catccatcaa ggaaaagcac aatcccgcca accttatcat aaacttcgag
    18661 aaaacgaact acatcaactc ggcggggatc gcgacgctca tcaacattat ccaggacatg
    18721 ggcgagaaga acggtcgggt cgccttcgtc gggctgtcca accacttcca aaaggtcatg
    18781 gatatcgtcg ggatttccga cttcgtcgac atcgtgaaca ccaacgatca ggccatggag
    18841 aaaatcaagg cccggtcctg acgcaccgtt ttcccactct cacaatacaa ttcacgcata
    18901 ccaccgaaaa aaaagctcga ttggaaaatc gagggctgcg gcctggcccc gagcattctc
    18961 gtgacgcgcc tcgggccgcc gagatgttga tgaaaaccga gcacacatgc gctccacgct
    19021 cgatttcgca tccgcacccc ggcagtccgc accatgcgaa cgcgggggat gattgccgtc
    19081 gttgcgacgg ctgcagttcc ggcgcgagcg accgtcgcca tcgcacaccg gacaagagcg
    19141 ccccctagca gaatgatccg gcgtgctgct gaagctccat ccggaaacct gcgggacgga
    19201 ggagcgccgg gttctgcgac atccgcaggt agccgagctc gaagctcccg ttgcgaaacc
    19261 gaagccggaa cagatgcgcc cggcgctgat agcggtcaat cacgcgcaca gtatagcaga
    19321 agccgtccgc gcccgcgccg gactcctcac gttcgcaaaa cacatcaaaa aagtaaatgc
    19381 cctgctccct gatccaccgc tcgatatggc cgcgcacccg atcgaacacc accgccctcg
    19441 tctttagaaa gaaaacctca ttcccatgat ccgccgcatg tctttccata aaaacctctt
    19501 tgtgctttat tttgggtttt ttgattgaca tcgtgaggga tcgaccgtat attactacta
    19561 acagaaactc aatacatgtc aaatcggggc acccaagccc cgtggcctta agttaccgct
    19621 taaggccttt ttattgtcca cccgacagga aaccgtatcg agtaaatcag gggaatgtac
    19681 tgatagtatg aggaatcgtc aagtttcggg ggcgtttttt ctgatttttt tctgcaaagc
    19741 tgattacaca agaatgaggc accatccaag taatggtgct caggtcggga tctcgattcc
    19801 atgcgtaagt atctctttta cgaggggatt actttcatta aactcatcga gtgtaaacgg
    19861 caatggttcg atatcgctcc cagccctgag ggtgatccgt ctgatcttat ttttatcggc
    19921 gattcgatcg ccgtcaaaac cgtccgaaac cagagcaata tctatatcgc tccattcatc
    19981 gtacgtgcct cgggcatagc tgccaaatac atacgcacgt ctgatccgta tgttgttttc
    20041 cgccaaatcc agtaggtaac gctgaatacg cgcctctatt gcatggggga tacgagccat
    20101 aaaaagaatt ccttgatttg aataaaatat cccgcggtat attcctcagt acatctgccg
    20161 taaaatgaat gcttaaaatc cggataccgt gcttcaagat tgaaatcgtt gatctcgtcg
    20221 agtagaattc tttgcgcatc cgtgatcaca agccttgtct gttcagccag gcgtaccagc
    20281 ttatgcaccc gaggcgggat ggcattatca ttgtcgaata cgtaccgagc cttcagagct
    20341 ttttcaacaa cgagatgccc aacaaacagg caccagtcga atttttatta gcatacagtg
    20401 tttccgccgt ttcaagatca tggcgggcac tttctagcca gtacagcacc tgttcatctt
    20461 tcgtcattag ccggctcccg gaatgattgc caattgatcc gataccacaa cgggtcattt
    20521 gtcaataaat ttcttattgc cgactctcac cctgttcatc gaagcagagc gaatctcaag
    20581 tgtcttcaag ccatatcgaa tggagatgtt tttccttccg ggatattcgc ctgattcgaa
    20641 tcccgatgaa cccggcaggc ggtttcatcg gccggaaacc ggatcgggtg aataaaactg
    20701 taccagcaat taaggaatag tcttatagtg ggacgatttt tatatttatc cccgggaagc
    20761 tcattacgga aggatgaagt aaccggggga agtatgaccc ggggcccact tacacgggtc
    20821 ccgacatgaa gatggagaag cgcggcctat ggccccgcaa ccacaacacg aaagccgatg
    20881 tcctcagtac gaaacgtggg aactatttta tatcgccaat atgacgaccc accaccaccc
    20941 ttacaggaac tgccgcgaat aacgcgctgc gtccccacga acggaccctg cggattttgc
    21001 cttgggctgt tcttgtagta atcaacggca taccaatcca aacaccactc cattacattt
    21061 ccgattatat catgcaatcc atacgcatta gcctttttct gcccaacagg atacgttttt
    21121 ctctctgaga aatgattacc ataccaagcg taatcctttg catcattata atcatttccc
    21181 cagtaaaaac cagtagttgt gcctgcgcgg caggcatatt cccattccgc ttccgttggc
    21241 aggcgcgcgg taactttatt ctttgtgccg aatcgtttac aaaaattcac ggcatcgccc
    21301 catgaaaccg tttcaaccgg gttgctgtca cccttgaata aactcggttt ggcgcccagc
    21361 acttcgtggt actgtttttg cgtaacctcg tatttcccca tccagaaccc gtccacggtc
    21421 acttcatgct gcggacgcgc atctgccagg tattcttcct tttcgggaga acccatcgta
    21481 tacgtgccgg acgggatcca tacgaatttc attcctccga tttcccgctc tttaacctcg
    21541 atctttttcg cgcacgagct ccaggcggcg aggatcgcga ctacacacaa cacgatcagc
    21601 gattttttca tttttcatta cctcgattga ttgtgcactc ttcaagtgtt agattacttt
    21661 cttgcgccgt tctttattaa aagttttact atatcttcat cactagtatt taaggacgtt
    21721 aatatcgtac gcccccattt atctcttgca ttaatatttg cgccatggtc gagcagcaat
    21781 tgtaccaatt tcagatttga gctatcacca cttggtgttg ctgcatattc gagggcataa
    21841 ccgcctctat tacaacgact attcggatca gcgccatatt cgagaagcaa ttttgcaata
    21901 tcgtaatttc gctgatgcca atgatttcct tttttatctt cataacccat tcctgcagca
    21961 tatactatca atggagacga attgaatttc tggttcggat cagcgccata gtgcaatgta
    22021 tctctcacag ttttcacatc aaggccaata accgcttcta cgagctgttt gttccagctt
    22081 ggatcccttc gctttctact catttccttg gcgaattgcg ccgagttctt ttcccataac
    22141 ttcctgaccg tgtcggcctc tttttcctgg ttcgatttcg caaaacagtg gatcaaaccg
    22201 ctataggccg gaccgaaata cggatttaag cttatcgcca tgaggaagct gccagcggcg
    22261 tttttataat tgcccattga cgattggagc gtcccgctca ggtgatgaaa catggcgttg
    22321 ttttggcaat acggctcccc ggctttcaga tgatacagcg attcctcaat ctccccattt
    22381 tctgcggcga tgatcgcaag cccggtatac gcatcgccga gcctgggatt gtttttaatc
    22441 gccatcttga atgccacggc cgcctgtttc ctatcacctt ccgcgaagta gcatttccca
    22501 agaaggttgc aggaatccgc cacacgcatg ttatttctaa tacaatacga tgcgatctcc
    22561 ctggcggtcc cataataccc gtcctggtac gaacggaaag cgatttgcaa catcttttga
    22621 ggattgtcgc catttgcagg atcgatcagc atattgctgc gcttcgatgc acacgaagac
    22681 aaaacaagca gccccaatac ggcaaccagt atcgcatata tcattctcat tccgttttta
    22741 ttcattgtcg cccccttgat aaatgagtaa ccacgcaatg tgcatgtggt cggcgcttat
    22801 agtgtgaaat cataaaattc taatttccaa aaccgcatgg cgcatgcctt cccggtcccc
    22861 tgtcgcaaaa gtcggatatt ggcagattcg cgagtcgaca cgacgcgcac gagctggtgt
    22921 gccggaacca ttcgtcccat tttgccgctt ttcttctatc aatgtttcgc attttttcga
    22981 acaagatcgg gtgccgcggc gttcccgcgc aattgcacgc ggacagttcc ctttcggctt
    23041 ttgcgtaatc cgcctgtaat ttacagccct cacagcggat actgctccca tagccgcata
    23101 tacctgcctt atccttaccc tttatttcat tgggcgtctg attcattacc ccgcccttgc
    23161 tcttgctcca gtcttgatac gctttggagt tctttcttgt gccgggattt gacgcgatac
    23221 tccgaggaac agatccagca cttgacgcct gattcgaaga cctcgacttt ccggatacgc
    23281 tactctttcg ctgctcacgc gccgcctgta tatcagaaac cccttggaga agggcgcttg
    23341 ccacctgaag ccccagagcc agattgtcga gtgtaaatat cgaactcgaa tcgtcgcccg
    23401 ccgatgcggc tttctgttgc gccaccatgt aatcgtgaaa cggacttcgc gccgattgga
    23461 cagcagaaaa atattcggcg gtttcttccg atgtgatctc aagcggcctc gttttgcgca
    23521 tggcggcatt tccgcccttc accgaccgaa catcatactg ttttggatta tacagaacgg
    23581 acggactcac tcttcccaac ggcacagaat accgtgcctg atcggacgaa tcaaagtttc
    23641 cacgggaagc gttttgcgcc gctattgaat cacagcgcaa tgcgactgat gttgctttat
    23701 gcggcaaatg aagacccgga ccaggcgcgc tcgacccgca aaaacgcttc acttcactgg
    23761 cataattgct tctggccttg tccatgcagc gatcatagtt ggttttcccg cccgaatacc
    23821 tgctattcca gcattcaacg gaattgatgg tttttttccc gacggctgcc accacctgag
    23881 cccattctgg taaaaatgcg ccatgtgaga gcattggaac aaaaatgaca cccaagaaca
    23941 agggaataca caccattttt actttcatgc ttttgcctct atttttttta caagtataca
    24001 accacaaaca cgacaacatc tcattcagac gggcccttaa cagtctcgaa atccatagga
    24061 aatgcgcagt gcggcactac agtatttcgc gatatcaatc gcctttcccg tatcgccgat
    24121 cttgtaatgt tggaagccga tatccagcat cttcgccggc gtgagccttc gtatctcgtc
    24181 cggggagtcg tactgctcgg cgaccttttt cttcgcggcc gtattgcagg cggtaaccgc
    24241 gacggcgcac accgtcacgg ccaacagcat tcgcacgcgt gcgttcattc cgttcatctc
    24301 aaaccccatg aatactcatg ataatatatt ctgccacaac gcggcacaac gcggcacaac
    24361 gcgacacgtc acgctacaat cgcatgagct tcttgatcac ctcccaggag tcgctcacga
    24421 atcgccagat catccagcat gatttctggg cgggctctat gcaggaccgc tggggattta
    24481 aacaggactt ctgccacgaa gacgcgccgg cggacgtctt gtcattggag gcggccttct
    24541 tttcgttgct ggcgcgctct cgagcctggg cttcgcgctt ccgctcggcc tcctcttgtt
    24601 ccttgcgatt ggcgctttta cggtccattt ccgcccgctt gctctttacc aggtcttccc
    24661 ggatcttctt gttccgcgcc tcgctgtccc gatcggcctt gtcgcgaagc tcctttgcag
    24721 ctttctgctt cgcgagtgtt ttctcgtaat tttctttctg ctggcggtcg cgctgctcct
    24781 tcaacttttg ctcacgctca agttttgccc gctgttgatc ttcccattgt tttttccgca
    24841 tttcctgctg ctttcggtgt gcgtcggcct ggtcctgctt gcgtttttcc tgctgttggc
    24901 gcagcttctc acgctgctcc atctgcgcgc gctcgcggtc tttctgttgt tgctggcgga
    24961 gctccgcatc cctccggcgc cgttcctcct catcgcgctt gcgcttttca aatgcctgct
    25021 gctcctgctt ccgacgttct ttttcgcgct cgaactgttc ctgttgcttt tttttgcgct
    25081 cctcgttctg gatgcggatc cgttcttcct gctctttttg cttgcgtgcc cgttcttcct
    25141 gttgcttggc tagcgcctgt tgtttctgac gctctttctc ttgagctact tccctttctt
    25201 tttctcgttc tttttccttt tgccgttttt tctccgcttc cctttctcgt ttttcctctt
    25261 cttctttttg tttctgtttt tgtatttccg cttcttcttt tgtatcatac tctgcatgga
    25321 gtattacatc ggcatcccac gtttcgacat acggactttc cttacatgcg accatggaga
    25381 tatcgatcgt cttgccggga cccgccgttt gtgcgcttcc ccatccccac catttctcgc
    25441 taacgctttt tacattgctt atccctttaa cagttattcc attaatcaat acgggtcgcg
    25501 agcaactgtt cactacctct atatacttgc aacttgcggt tgcatgtggc cgaaatcgca
    25561 accgaaaaca gcgcggcgcc ccggggagtg tcgatgttac cgaccaggcc caagcctccg
    25621 tataaagtcc aaatgataca attaccacca ctgcagcaat caggacgctt tttgaaattt
    25681 tattgctcat gaaaacaatc ctcacgatta ttgtttagtt aatgcatcat gcatattgaa
    25741 ggttacgcat tcctcatgtg ggccggtact tcgcggaccg gttaaactca cgccatctcc
    25801 atcatccgca aatgctcctc gatcatcgcc ttgcattctt tcaggatcgc gtcgaagcgg
    25861 ttgccgcggc ggatcacgtc gtcgtcgagg acgcagagag tgccgaagac gcgcccgtcg
    25921 cagcccatcg caacataacg catgccgctg acaacccatt ttccgcaccg atcatttcat
    25981 attctgcgtg tcgaaattgt agccgccggc ctcttcatcc ccgcgggcag cgggtttttt
    26041 ggcggctgtc gtctttgttt tgggagcgca tttgttttgc cctccctggg aaaggcttcc
    26101 caggtcccac gcgcatggag attcagcctt tttcggcgcc gtttgggccg gcgcgacctg
    26161 ttgaggcgct ttgctgacct tcggcgcgct tgattggcgc ggaacactcg gtctcttcgt
    26221 cgattccgcc aggttcgcga cggcggggat caggtacccg ataacctgca gcaccggaat
    26281 cgcaatatcc atgcacgagg aaaccttgtt cccgaagccg ggggaatccg cagggggcga
    26341 actgtccgcc cggccgtcgg tcggcttctc gataggattc tccgcgcggg cctggtttcg
    26401 cgattcctcc tcttcccggg ccagatccga tcgcctcttt ttcccgtcct tggacggatc
    26461 ataattctgc tgtggtttcc gcttgagata ttcggccaga cgcttgtccc cttccttgtt
    26521 gttttgcaat gcggcaatgc agatggactt ccgctcttcg tacttcgccc tctgggcggc
    26581 ataatctttt tcagccctgg ccttgtcctc ttgagtcacc acgaacgggt tttgtatcgg
    26641 ctcatgcacc gtgcccatgc acccttcgat gtaacggcgg tcctttgcct cttcctgtcg
    26701 cttgagttcc tcatccgcac gcatctcttc ttgggcgtca gccacggcgt cgagatccct
    26761 cgtgccggac ccaggcaaat aacccgacat catggtgacc gaagtggcgg acaagagatt
    26821 ctgcgaattc tggcgcgtat ttacgccgtc cctgttcagg tcgttctgca atatcgggtt
    26881 tccatccgat gccgaacgcg accccgccag aaaattgccg aattttttca tattcctggc
    26941 gacaatatcc ttcgatccgc aggcgacacg acccccgatg tcagaccact tcgttttcga
    27001 acaggcaccc ttgacgaaat ccggctgcat ccaatcggca gatttattga tacccgcagc
    27061 caacgagtcc aacacaccgg cctgtaacga cgaaacttga gtgaacacta aagagccagt
    27121 gattactgcc catgccatcc ccttcaccca catcattgtc atcaccatga gcccatgtaa
    27181 aattcttcgt agtatatcaa tccatgcatc atatgccgta cttgatttta tgtactgcgt
    27241 ataattcctc cgcccgtttg gccagggcgt catcgcgtga tcgtttacag gtgtgcaata
    27301 aacgcttgta ggcgttcacg aatttcggat tgaggttgat cgcctgaata taacaaaatg
    27361 cagcgcgttt atattcgccg acggcattgt acatatcgcc ccaattgagg taatacacgg
    27421 cgttgtttcg acccgcccac gggacccgtt tcaggcttgc gagggcgagc gaaacgtttc
    27481 cctcatccag cgcgaccgac gagagcgtca gatacgcctc gccgttgttc cccgccatct
    27541 cgagcgccgc gcgggctttc ctgtaatcct tcttgacgta atagcatttc cccagcagca
    27601 cgtaggcatc cgagcggctg tagttcttat agatgcagta tttggcgata tcgatcgcct
    27661 tgccggtatc gccgatcttg tagtactgaa agccgatatc cagcatcttc gcgggcgtga
    27721 gcttttttag cttgtccggc gaatcgtact gttccgcgat cttctttttc gccgccgtgt
    27781 tgcaggcggc caccgacacg gccaacacta ttgcgaatcc cgtgtttaac actttattgc
    27841 cacgcatcac gatcattacc tccaggcgtc agtgaacgtc ccgtcccggc cgaccttccg
    27901 cgctaaaatc gtttcacccg ataccgaaat tgccagagga tcgaagagtt tctcgtaaat
    27961 ctgaaaatcg tcgcgtcgtg tcgcgctagc gacaatttgc atcaaacgtt tgctgcgagc
    28021 tctccttgaa cgattgcgga cagtcggcgc accctctggc gcgctgctgg atctcgttca
    28081 tcttttgaca ccatttcccg ccctttcccg cctgtcccgg agtttcttgc gcgggaagcc
    28141 gacccaattg gtcgatgaga gccatagcgc cgcaacaatt ctgggcgccc gctcccgagc
    28201 cggttttccc ctgaatctgc tgcatcttgt cgattcccga ttgcagcgct tcggcggctt
    28261 gtcctacccc aacggccacg tcgagcatct tgtctatcca gctatcattc gaaagctgcc
    28321 tgttgtcctt ggcgaggctt ttaaggacct tctggtccag cgacgcgacg aaggccttgt
    28381 tgtccgcctg ccactcacgc cacatgtccg gctccttttt gcgctcctcc ttcgttttgt
    28441 agaacggatc cgcgagcgcc gcggcgcctt tctggcttcg ctcgagctgg tcgatgatcg
    28501 cgtcgctgct ccggagaaaa tccctaccag cgacatccac ctcgctcggg acgcccagcg
    28561 gcctgttgct tcgagcgcgg aagctttcgc tgccccgatt gtcctcggcg attgcgtcgc
    28621 cccgcaggga cggcagctcc acgcccgggg ccgctttgaa atacgccttt ttagcgagcg
    28681 atttgagcgt ggagggaagc tcatcgattc caacgctcag gcagtccttc ttggtcggga
    28741 ccggggtttt ttttcgaaca tcgtagggga ggaacgcgcc gtcgggactc ttggcgcaca
    28801 gaacgaggct cttctcgtac tcctttttcg ccagggcgag tatggcggaa agcgatcctt
    28861 tgcccccggc gagctccgcg aagagcgggc cgacggcttc gacgagcgcc ttgttcccgg
    28921 ttttttcaaa gcattccacg aggccgttgt acgcgggaac gaatccggga ttgatgctga
    28981 tcgccttcag gaacgcacac atggccgggt aaccgctgcc catggcgagg ttgagcgcgc
    29041 cgttcaggta gtgatacatc gcgcttccgg ccgcgtatgg ccgcgcctcg cggagaatat
    29101 acagcccgtc gcgaacattt ccctgctgaa gcgcgatgag cgccagggac gtatacccct
    29161 ccgcccgccg ggggctgttg ttgatcgcgt acttgaacgc gatggaggcc ttgacgtaat
    29221 cgcgctcggc gaaaaagcac ttgcccatca ggagaaacgc ctcgccggtc tccatcttgt
    29281 tgttgatgca gtgcgagagg agctctcgcg ccttccttgt atcgccggcc tggtaatagc
    29341 ggtaagcgcc gccgatgaga cgattggcgt cgaacaggtc ttccttgatg atcttcacct
    29401 tgacgtttga cgatgacgat gtcccgcacg aggcgtacag gcaggcggtc cccgcgagac
    29461 agacggcgat gattttcctg acggcagtgt tcatttgggt tcgctccttt ggcataaaaa
    29521 atcgtcccgc gtacggat
//
LOCUS       DAORHF010000014        29514 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_939_length_29514_cov_6.293289, whole genome shotgun sequence.
ACCESSION   DAORHF010000014 DAORHF010000000
VERSION     DAORHF010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29514)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29514)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29514
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_29514_cov_6.293289"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="PKJ16_02365"
     CDS             <1..1394
                     /locus_tag="PKJ16_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HNV45856.1"
                     /translation="SGSLVLQNNNGDDLTIRGDGPFKFATPIQRGGEYRVTIRKQPDG
                     PECGVYNASGTALSEVTDIVVVCSYKSYPVRVTVRGLAGKLTLANNGIDTLEIDSDGS
                     HVFNLPIVKGGRYRVTVDSKPTIQSCTVNNAEGPVNGPVELDVNCSTISYKISGTVRG
                     LKEGSIILQNNAGDDILMDANGNFTFHDPVVKDSNYTVTIRSQPGIRPCRVINGGGKA
                     TRDVTNVSVLCSYRVVLYSAGLYNGDMGGPDGTDAKCAAMGNVSGLDAPYTAFICKSG
                     SVSVHTMPEVYGIPKHLPVTTPGGALIAQNWEDLVNGKLRMSLETLVDQGSKNTTFWS
                     GCDATGNYKANAKNCGNFYSYIFPPGPIPATWVPAWKTTIDDQRAASKGWTGSMMLTG
                     NGWLSQREIACTQNAYLLCAAYGPEPASVLTAYLRFPPSHSASLLAIMIGVVGIALAA
                     IVVIVIVRKSRST"
     gene            1402..2307
                     /locus_tag="PKJ16_02370"
     CDS             1402..2307
                     /locus_tag="PKJ16_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV45857.1"
                     /translation="MNRNETTRLVDAGLRAAETDMADEDDIWSYNSNDKVDIGERLAS
                     VIRTLHRALPQARKMRALSLGSSSEPQFRILETAFRGGLYLMDIERHALDIVEERIQR
                     QYTDHVFTMQGSYLDFIETPGFPGRLRSTWMHGRKVDLITMHHSLYYCEESTWLPLFE
                     TLFRTVLAPRGAIHAVLMANTSRDTHTTTWLYNHFAGKFFGIRNNQSLPVLKRELERS
                     RVFDDSQILISTSSVNFFVGDFEKFMKVVWMILLYPNVHPFTESQKVEIIRFVIDTFW
                     APKRPLVQTQHHLVCYRGLDFKGMI"
     gene            2475..2753
                     /locus_tag="PKJ16_02375"
     CDS             2475..2753
                     /locus_tag="PKJ16_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45858.1"
                     /translation="MIETLLPAFIMESAARYNAVVILAAVALFACLLFAGAAAGVFLA
                     FRIDTAMKRRGRNLEMALFAVILLLVLGVALAAFSVAVLFAVAHLRCG"
     gene            2852..3940
                     /locus_tag="PKJ16_02380"
     CDS             2852..3940
                     /locus_tag="PKJ16_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HNV45859.1"
                     /translation="MRGTKRLSRAACAAIALAVCGALLAACRERPHETVDFVMGTVVR
                     QTVYGPSAETAAREAVERLRALEAALSFHDAASDISRLNRSSGGPQVTLGPEAIGLLE
                     RSLEFSRISDGAFNIMVGPLVRRWKVTADRPDVPPPAEIASLVRLASYRDLSVDRDRR
                     TARLARRGQMADLGGIAKGYASDVILALYRARGVRSAIIDIGGNIGLLGSRPDGRPWN
                     IGVQDPGRERGSYIGYLSLADNSVSTSGGYERFFVAGGRRYSHIIDPRTGYPADSGLS
                     SASVIAPSAEESDALSTAVFVLGASKGIGLIQRRPGVEAVCITTDGTVIVTEGLRGRF
                     TLTAPEGTYRVEYRYRVGPDAAGPARQR"
     gene            3945..5264
                     /gene="rsxC"
                     /locus_tag="PKJ16_02385"
     CDS             3945..5264
                     /gene="rsxC"
                     /locus_tag="PKJ16_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HNV45860.1"
                     /translation="MGKAMKAFRGGIAAPHNKHTENCATEAMGVPARIIIPMVQHIGA
                     PCAPLVQKGDRVTVGQKIGDNDAFISSPIHASASGTVADVRPILYAGGVEVVSVEIET
                     DGAQETCPDIRPPATDRESLIKAIRESGIVGLGGAGFPTAVKLSPPADKKIDILVLNG
                     AECEPYITSDYREMMENPGGIIEGARIIMELAKIPRALIGIEANKPEAIRLLTDTVKD
                     DPAIDVVTLRTRYPQGGEKQLIYATTGRRVPAGGLPSDIGVLVQNTTTASFIAGFVKT
                     GMPLIKKKVTVDGSAMATPKNVEVLIGTPLKDVFEFCGGFREEPVKIIMGGPMMGVAQ
                     FSLDNTVIKQTNALLALGAREVALKPEIVCIKCGRCVSACPMGLLPLYLNACMERGLF
                     ERMADDYHVSDCIECGCCSYVCPSARRLVQSIRYGKAELRKLAAKKK"
     gene            5308..6255
                     /locus_tag="PKJ16_02390"
     CDS             5308..6255
                     /locus_tag="PKJ16_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV45861.1"
                     /translation="MKTGILIAGSSPHLRSDATARRIMLDVLIALVPATAAAVYFFGV
                     RAALVIAVTTAASVLAEFVSNKIMKKPITIGDLSAAVTGLLLAFNLPPGIPLWVAAIG
                     GVFAIVVAKQLFGGLGMNFINPALAARAFLMASWPVFMTTWTTPMVDAVSSATPLAII
                     KSAEAAGATPPSIAQLFLGNVAGCLGETSALAILVGAAYLVVRRVITLETPLAFIGTV
                     AAMTWVLGGKGLFSGDVPYHLFAGGLMLGAFFMATDYPTSPITWKGKIIFGVGCGLLT
                     SVIRLYGGYPEGVCYSILLMNLAVPIIDKFTVPKSFGGA"
     gene            6259..6843
                     /locus_tag="PKJ16_02395"
     CDS             6259..6843
                     /locus_tag="PKJ16_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HNV45862.1"
                     /translation="MRDIVKTTIPLLALCLAVGLCLSYVNALTKGPIEKRKIEDAEKK
                     KKEVLPEAQTFEEIADWKTAAPEGHVLTGAYRGMRDGKTVGYVFSAAPKGYAGAMTVT
                     IGIKRDGTVGAVSLGDNKETPGLGTKAGEAPFITQFAGKTIATPIELVKMTASGNQVQ
                     AIAGATISSRAMVVAVQHAAAVARELLKREGGKP"
     gene            6840..7493
                     /locus_tag="PKJ16_02400"
     CDS             6840..7493
                     /locus_tag="PKJ16_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HNV45863.1"
                     /translation="MSLLAIIKNGIVDENPTTRLVLGMCPTLAVTTSAINGIGMGVST
                     LAVLMGSNIVISLLRSVIPDKVRIPSYIVVIAGFVTIVQMTIKAFAPSVDQALGIFIP
                     LIVVNCIILARAEAFASKNGVLASAADGLGMGIGFTIMLLIIGGAREILGNGSIFGIA
                     LYGQTASPALMMILPPGGFLAFGVLIGIVNMLSEKKITQIGCDSCGAPCKRNAENRG"
     gene            7499..8083
                     /gene="rsxA"
                     /locus_tag="PKJ16_02405"
     CDS             7499..8083
                     /gene="rsxA"
                     /locus_tag="PKJ16_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HNV45864.1"
                     /translation="MAEQIITSFLAAVLIENFVLVRFLGICPFVGVSKRLETAVGMGG
                     AVIFVMALASAITWLIQRFVLTAFQVEYLQTIAFILVIASLVQFVELIIKKVSRGLYD
                     SLGVYLPLITTNCAILGACIINIQKGFDFINAVLYGTFAGMGFTLAIVLFAGIRERLE
                     LFSDVPANMKGFPISLVAASLVSLAFFGFQGLIK"
     gene            8096..8971
                     /locus_tag="PKJ16_02410"
     CDS             8096..8971
                     /locus_tag="PKJ16_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HNV45865.1"
                     /translation="MIMNEIIIATVILSALGLFFGVGLSIASKIFAVTEDERVAKIRA
                     LLPGANCGACGYPGCDGLAVALVEGKARVLDCPVGGETAGDAIAEVLGLAVEKGEKMV
                     ARVLCKGDCDVSRDKFSYDGLQDCVAAAQIFGGSKSCRFGCLGYGNCQRACPFGAIDM
                     IKGLAVVDEARCKSCGLCVAACPKALIEMAPMTGRHFVFCKSKDKGAATKKACDVGCI
                     GCTRCFKTCKYGAIRMEGPLAIIDPCVCTNCGECVPVCPTHSIVRILAPGETAPTAVP
                     VGEEATPASAEQSPS"
     gene            complement(8937..10268)
                     /locus_tag="PKJ16_02415"
     CDS             complement(8937..10268)
                     /locus_tag="PKJ16_02415"
                     /inference="COORDINATES: protein motif:HMM:NF003376.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HNV45866.1"
                     /translation="METYAESHDAPGGRAAAPTVEAKPAEKPVEGAKRDDGAITLGAD
                     AIEKLKSDLIAEIHKDLKQNLTHEIVREIIDVLGERFLGARPASTRGADGAADGTGPA
                     AAGASPKDNLPSAAERIGAITAFAVKKEPASIPVHRVKLGATKAEGGTRGRTLEIGGA
                     TAMPFHLWEGAMPNRPLVAMEVFDRVSEKYPPVLRKFWGDLVERPAEMAKACVEKYGA
                     DLISVRLEGTHPEKGNRSAAESLALVKEVLAAVDVPLIITGHNNYERINEVFKEIASG
                     CAGENLLFNWVEQDNYRTVAGAAIAYGHSLVAQSPIDVNIAKQMNILLTNMDFKADRI
                     VIDPMTGAMGYGIEYTYSVMERIRLTGLGGDAMLASPMIVSPGQECAKLKELKADAAS
                     FPSWGDLDKRAAYWELATATSLLYAGADVLIMYHPEAATALKTAIARLMRG"
     gene            complement(10263..12440)
                     /gene="acsB"
                     /locus_tag="PKJ16_02420"
                     /pseudo
     CDS             complement(10263..12440)
                     /gene="acsB"
                     /locus_tag="PKJ16_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
     gene            complement(12433..14394)
                     /gene="cooS"
                     /locus_tag="PKJ16_02425"
     CDS             complement(12433..14394)
                     /gene="cooS"
                     /locus_tag="PKJ16_02425"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HNV45867.1"
                     /translation="MDKEKDKTIRAAQDRATDRASIETLEKAERDCVTVSFFRMDQQK
                     NQCGFGKSGVCCKICHMGPCRITPKAPRGVCGADADTIVARNFLREVAGGTAAHSDHG
                     RHLVLLLKKIAEGKGGGYVIKDEPALRRIAREYGLETENVATQELALQLADVFLRDFT
                     AQEEHLETLKLAPVKRQAVWERAGIAPEGIDRMVVESMHRTHMGVDHDPHNILLHSFR
                     TALADGWGGSRIASQVSDIIFGSPSPVKSAANLGVLEKNKVNVLVHGHEPALSEMLAA
                     AAGDPEVTARAKEAGAEGVNLAGICCTANEVLMRHGIAVAGNFLQQELAIVTGAVEMM
                     IIDVQCCMPGLPEVAASYHTEIVSTSDIAKTVGAHHAAFNPDDAYASARDLLFRAIAN
                     FKRREPKKVFIPAAQSPLVAGFSVEAIKYMLGGRFRASFRPLNDAIIQGRIRGVAGIV
                     GCNNPKQQLDHYINILTKELLKNNVLVLKTGCAAIASAKSGMLTPEAALEHAGAGLRE
                     VCEAIGMPPVLHMGSCVDNSRLLEAATEVVREGGLGDDLSDVPVVGVAPEWMSEKAVS
                     IGCYFVASGVDVVLGNPFYISGSETVTNYLNGGSAEAFGASFHLHENPLEAAEAILAI
                     IDRRREKLGINKKAERKLYDMKDRRELDV"
     gene            complement(14486..15250)
                     /locus_tag="PKJ16_02430"
     CDS             complement(14486..15250)
                     /locus_tag="PKJ16_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV45868.1"
                     /translation="MGYTIAITGKGGCGKTTIAGLIVDALVRLGKTPVLAVDADPNSC
                     LDTVLGVRVEKSIGTIREEAREEAAKGMASGIAKRDLLELRISECLVEADGFDLIAMG
                     RPEGPGCYCYANNVLRDVIAGVSASYPYVVIDNEAGLENLSRRIVASVDLLILVAEPS
                     INGVKTVQRLHELAGEMGIGYTSLAIIVNRMKGDSLIENARLMIDGIGAHHVMLLRDD
                     DEIAAASERGSSITGLSTENNVSSQIYAFIQRVLSN"
     gene            15622..17526
                     /locus_tag="PKJ16_02435"
     CDS             15622..17526
                     /locus_tag="PKJ16_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45869.1"
                     /translation="MLAGNHISAVSLAFLGACAGTLLVGVLIYGIIYHFTRSPLHAGV
                     ALMGLFGFVFVAGELGVALCHLANDPLLGRRMHRMQALAAAFFIFGVPYVASGLTDLS
                     HAWRSANRVVSYASLAIVAAIVVVAFAMPDFFISQTSGGMESLHRLSHDAVRGRTGPV
                     YRLRDIVIAALMGYALVMFVFDLLWHRNYRYISLPLAGSVTAITSAFVDYFYIYRGVD
                     GPFPARESISYFSVGITIMIVLCMVGVVLRFIDQTRDIEKAQRIESLGLFAGGIAHDF
                     NNLLTAVIGNISLARLDAGAGRAAQALDQAEQASFRARDLTRQLLTFSRGGEPVTRSV
                     PVQDMATETAHLVLGGSRIECVFNIAPDVRNVIADPGQIGQVLQNIILNARQAMADRG
                     RIDITIANEDAAAGRRRERHAGPYVRIAIADTGPGIHRRDLARIFDPYYTTKTAGNGL
                     GLAIAHSIVHKHHGSITVSSRAGVGTTFVILLPATDAAEHGASDHAPVLATPGAHGAI
                     LLVDDEESIRAMGRMMLERLGYSAVTARDGREAVEAYRAANAGESPFRCVLLDLTMPD
                     GMSGREVLAELRAIDPGVRAIVMSGYSNDPAIAAYREYGFRASLAKPFGIDDLTRAVD
                     GVMADGGPTA"
     gene            17594..18883
                     /locus_tag="PKJ16_02440"
     CDS             17594..18883
                     /locus_tag="PKJ16_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45870.1"
                     /translation="MKNMWIIPLSVMLIIGVTIGIVHSQKARKPVSIAAPKLFGSEPL
                     TKINHQDVPNNAMVLSDGSVLISVGQTGNPTEAQTKMESMMKNEKRMPAVPTYACSTF
                     GWAFAMKVNAREQLEWMQVIYPESVRGLYDPQNQGWQTNQLHFATAAPTRDGILFGGT
                     APVMKPDDSSQLPVLIKYRNNGKKAWEKVYAEFAVADEKFLRSGFMNVGETPQGITAI
                     IFVKKDYFWVKMDANGAILPGSKQPFAFRGSSALEARFAPDGSLYTVDADGGAINVSR
                     HDANTGAGLWRKKANDSGSCGLIGTTNIVVTRDGGVAVCGYRESGDTRDVIAARFDAN
                     GNRQWVFQYGAPGASHWPRGISIMPSGDIVVASSCDQGNSRYTRLILLNAQGKEVCAN
                     NYSGISGNSYFAGNFLIGYGCRRSEDVCVKYIPAICK"
     gene            complement(18923..19327)
                     /gene="fosX"
                     /locus_tag="PKJ16_02445"
     CDS             complement(18923..19327)
                     /gene="fosX"
                     /locus_tag="PKJ16_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019555054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FosX/FosE/FosI family fosfomycin resistance
                     hydrolase"
                     /protein_id="HNV45871.1"
                     /translation="MVRGISHITLIVRDLEKSSAMLATVLDAEEIYSSGDRTFSHSRE
                     RFFLVGSVWIALMEGPALTARTYNHIAFRIDDAEFATYRERVTKLGLDIDNDRSRIAG
                     EGRSLYFYDYDNHLFELHTGTLEERLRAYTGQ"
     gene            complement(19334..20395)
                     /locus_tag="PKJ16_02450"
     CDS             complement(19334..20395)
                     /locus_tag="PKJ16_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45872.1"
                     /translation="MRLPNAFTATAIGSFPHDNPEQPLDIIFASMPDAPVWPQLPKTG
                     ALESMEIQYAEGMPRAVIDTGKSRLHFDTSGDYSEDFADFYERYMTLMESGGDFGPLA
                     IGPAHSKGIYALEKRLQAAGKKHPFVKVQVTGPCSFSLTVTDENKRALYYNEEFRDVI
                     AKAIALKSRWQIRKFAPYAEQVICFVDEPILSAFGSSTYIAVKRDDVIALLAEQVEAI
                     HADGALAGIHCCGNTEWSIPVEAGFDIINFDAYAYGETIALYPDAMKQFIGRGGVLAW
                     GIVPTSEAIRSNDAASLADRFGPLAADLAKKAGIDLAKIYASAMVTPSCGTGSLPVAD
                     AVKVFEVLKALPGELRTRY"
     gene            complement(20648..21430)
                     /locus_tag="PKJ16_02455"
     CDS             complement(20648..21430)
                     /locus_tag="PKJ16_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HNV45873.1"
                     /translation="MMKIAVSGKGGVGKSTIAAGIALTIARRGGSVLAVDADPDANLA
                     DALGIPRAAQGSIVTIARHRELVEERTGAKAGEYGQIFKLNPTVSDIADDYAYAHRGV
                     SLLVLGAVKGGGAGCACPESSLLRALVQDLVLRRDETLVMDMEAGIEHLGRATVRGVT
                     ALVVVVEPGMRSVETVKRIVAMAAEIGLARIAVVVNKVRSDDDERYLRESLPGLDIAA
                     SFPFSDRLLASDRDGVAVIDGMDDGMRARLEALVDFLEGKYS"
     gene            21600..22229
                     /locus_tag="PKJ16_02460"
     CDS             21600..22229
                     /locus_tag="PKJ16_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45874.1"
                     /translation="MGVQDGYTPREALEISSSLLYVVMLCVITCVASLFQVFAQVSPA
                     GIVFPFALNLGAIALASVIHSRKKARRDSELLQWGVALLTITAPVLAKYNYARTFDWT
                     PAGWTFAAQSYNSSILLVVFVVLLQLLYNRRLFICCALYAFAHWIAFFVIAYANGAAL
                     YFYALSNDAPRLDGVIVLREVFYIFCVVVIAIVAYRNIASAPGHDGARE"
     gene            22373..23968
                     /locus_tag="PKJ16_02465"
     CDS             22373..23968
                     /locus_tag="PKJ16_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV45875.1"
                     /translation="MKHSDYTARQVLEIKSSFMFFILVIVLAFFGNGFQMLAKVTLAG
                     LLPSIMLNVSIIVMAFFVYMRKRALRSAAVFEWILGFVATTGIIFAKYNYAKSYDWST
                     VGWTYAAQSYNTSIYLAIFLVLLQLLYNRKLFIFYAIWGLAHWALFFALAVMNGALVH
                     PEAIGPDGIPFLDGVIMLREIFFIIAVGFISFVCYRNIPITQEYDERTTRQRELIEKQ
                     AAEQRALVVDIKEKMNILFGQVDEQNQLVGDFNEKMQSQSATFEEMSATLEELLASAE
                     NIHVSSVDQIDGNVKMETIVSEFRSIKQETKGNLAATFQDIEAVVNRTTQANERLQDV
                     EKTIGKIKAQATRIAETVTLIVDIADRVNLLSLNASIEAARAGEYGKGFAVVADEIGK
                     LAYQTAESIKEIERVLSLSAQTTEEGVGVINSTAEMIKGMIMNMGASSNKIKVLQESI
                     FIEEKYINIIIDQMTKNIQLAKNIGMGTDEQKSAIESSARAVEHVNEIVMAMAKEISE
                     LALTSQNILDNARDLMARAETIT"
     gene            23988..25163
                     /locus_tag="PKJ16_02470"
     CDS             23988..25163
                     /locus_tag="PKJ16_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV45876.1"
                     /translation="MNFAVFFTVALSLYAAVNAYIFVRGWRALVPAVVSRPSFTAAFL
                     FLSLSYVAGRFLEKVALGMVSDFLVWTGSLWLALMFYLFLAVVAIDLARLANRLFPFF
                     PRAITADPARAAAVTAVGVCVAGTLIVLGGFINTLLPRTTELVVPIDRPSWKGRTMTA
                     LLVSDVHLGTVIGRMRLSALVERINACAPDLVLLAGDILDEDVGTVMRKDLGEILKNV
                     RARHGIYGITGNHEFIGGVERTARFLQDSGIVMLRDWAVKIDGALYLVGREDRSVTRF
                     GGPERAPLDRLLAGLDRSLPVIVMDHQPYDLKHAAARGADLHLSGHTHAGQVWPASCI
                     VRAMYEVPSGAKRIGATLFYVSTGYGSWGPPVRIGNRPEIVVVRLTGPGAGQKPVCP"
     gene            25284..25625
                     /locus_tag="PKJ16_02475"
     CDS             25284..25625
                     /locus_tag="PKJ16_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45877.1"
                     /translation="MKIVTKKVENVIIVYPPSWLDLDTSAQFRSRIAKLIDAEDECHL
                     LLNFKDVKHINSANLSELIKAKKKLEKSKRAMKMCGVHDKVKDVFEVVNFNTFFEIFD
                     TEDEALFSFKN"
     gene            25625..26518
                     /locus_tag="PKJ16_02480"
     CDS             25625..26518
                     /locus_tag="PKJ16_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HNV45878.1"
                     /translation="MPRVFIIADIEGSSGCWNHAGSAFGTDEWARACVGMTKDVDAVA
                     RALFAAGVSSVTVKDFHRTGYNLLPRRIDRRARLIMGYRRGGVPGIGDPGGPGLLMMI
                     GMHAPSGTDGFIAHTMTSRIARLEVNGALMSEAQLFSASLAPYEIRPVFFSGCPVACA
                     QAQSVLRPITIHSIDKSAGPDRFDADMWRTELAAAAVRALGNDATVPYRPEGPFDARI
                     VMRDGEEAAKKLASRWGLDHEGPVIRVQAITIHDLYMALIRMCYLTPFIERILPAGLP
                     LYNCAGWAGQQWVRWRTRAEG"
     gene            26578..26721
                     /locus_tag="PKJ16_02485"
     CDS             26578..26721
                     /locus_tag="PKJ16_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45879.1"
                     /translation="MNDHLVINALVTAAKQDLSRGIKREDLLQSLANVVSGDIYRKIK
                     DRI"
     gene            complement(26729..28585)
                     /locus_tag="PKJ16_02490"
     CDS             complement(26729..28585)
                     /locus_tag="PKJ16_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HNV45880.1"
                     /translation="MIFVENLSKSYGRQLLFDAVSFRVNPRERIGLVGRNGHGKTTLF
                     RIITGEEGPDEGSVSIPRNYRMGYLQQHLAFSRETAVAEGSLSLPDEERGETWRVEKV
                     LSGLGFGEREMRCHPRELSGGYQIRLNLAKVLIAEPDMLLLDEPNNYLDITSIRWLER
                     FLTSWKGELVLITHDRTFMDNVVTHTMGIHRRKLRKVAGGTDKLYSQIAMEEEIYEKT
                     RANDEQRRKEIELFVTRFRAKARLGGLVQSRVKMLEKMGQRDKLENIKTLDFSFIGVP
                     FTPQYIVNIEDLSFGYETDRPLITGLSMSIRARDRICVVGKNGRGKTTLLKLIAGALA
                     PDAGRVSINPKAVMGYYEQSNVQVLKDSRSVLDEVMNANPGLDRTRAWTICGSMMFEG
                     DDALKKIAVLSGGEKSRVLLGRIIAAPVNLLLLDEPTNHLDMESCDALLAAIDNFDGA
                     VIMVTHNEMYLNALADRLIVFHNDTVRAFEGGYQRFLEKGGWGDEEAVLRQDAPSKPA
                     GEGRKIGKAEARKIRSKIVAERARALKPLETKVSEIEGAIQRLEKELESHTGEIVTAS
                     QEKNGKRVSELSQLIHRCRAEIDARFDELEESTLALEQASAHFDALLSEVEG"
     gene            28882..29220
                     /locus_tag="PKJ16_02495"
     CDS             28882..29220
                     /locus_tag="PKJ16_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45881.1"
                     /translation="MKKKLLVIGLIVSLVVPVLYATDAVAEYKFTVKNSSKSKIVGLF
                     AREAGKKEWGAFDVGSGIASGKTETMHWDASTNSSGCVWEIMAKYADGSSSAPAKFDF
                     CKETDIEFTD"
BASE COUNT         5237 a         9253 c         9394 g         5630 t
ORIGIN      
        1 tttccggctc gctcgttctc cagaacaaca acggggacga tcttaccata cgcggcgacg
       61 gcccgttcaa attcgcgacg ccgatacagc ggggcggcga ataccgtgtg accatacgca
      121 agcagcccga cgggcccgaa tgcggcgtat ataatgccag cgggaccgcg ctgtccgagg
      181 tgacggacat cgtggtggtc tgctcctata aaagctaccc ggtgcgcgtc acggtgcgcg
      241 gcctcgcggg aaagctcact ctcgccaaca acggcatcga cacacttgaa atcgattccg
      301 atgggtccca tgtctttaac ctgcccattg taaagggggg acgataccgg gtcaccgtcg
      361 attccaagcc gactattcag tcctgcaccg tcaataacgc ggaaggcccg gtgaacgggc
      421 ccgtggagct cgacgtgaac tgctcgacca tatcatacaa gatcagcggg acggtgcgcg
      481 gcctcaaaga aggaagcatc attctccaga acaatgccgg cgacgacatt ctcatggacg
      541 cgaacgggaa cttcaccttc cacgatcccg tggtaaagga ctcgaattat accgtgacca
      601 tcaggtctca gcccgggata cggccctgcc gcgtcatcaa tggcggcgga aaggcgacac
      661 gggacgtgac aaatgtgagc gtgctctgct cgtaccgcgt cgtcctctac agcgccggcc
      721 tgtataatgg cgatatgggg gggcccgacg gaacggatgc gaaatgcgcg gccatgggaa
      781 acgtctccgg gctggacgcc ccgtacaccg catttatctg caaaagcggt agcgtatcgg
      841 ttcataccat gcctgaggtg tatggaatcc ccaaacacct tcccgtaacc acccccgggg
      901 gagcgctcat cgcgcagaat tgggaggatt tggtaaacgg caagctgcga atgtcgcttg
      961 aaacgctggt tgaccaggga agcaaaaata cgactttttg gtccggctgc gatgcaacag
     1021 ggaactataa agcaaacgca aagaattgcg gcaactttta tagttatatt tttcctcccg
     1081 gaccaattcc tgctacgtgg gttcctgcct ggaagacgac aattgatgat caacgtgccg
     1141 cttcgaaagg ctggaccgga tcgatgatgc tgacgggcaa tggctggctg tcgcaacggg
     1201 aaatcgcctg cacgcaaaat gcgtacctcc tgtgcgccgc ctacgggccc gagcccgcgt
     1261 ccgtgctcac ggcatatctg cggttccctc cgtcgcattc ggcttcattg ctggccatca
     1321 tgatcggcgt cgtcggcatc gccctggcgg cgattgtcgt gattgtcatt gtcagaaaat
     1381 caaggagcac gtaaacgccg catgaaccga aacgaaacaa cgcggctcgt cgatgccggc
     1441 ctcagggccg cggaaacgga catggccgat gaagacgata tctggtcata caacagcaat
     1501 gacaaggtcg atatcgggga gcggctcgcg agcgtcattc ggacgctgca ccgcgcgttg
     1561 ccgcaggcgc ggaagatgcg cgccctctcg ctgggatcga gcagcgagcc gcagttccgg
     1621 atcctcgaga ccgcgttccg ggggggatta tacctgatgg atatcgagcg gcatgcgctc
     1681 gatatcgtcg aggaacggat ccagcggcag tacaccgacc acgtgttcac catgcagggg
     1741 agctacctgg acttcatcga gacgccgggc ttccccggcc ggcttcgctc gacgtggatg
     1801 cacggcagga aggtcgacct catcacgatg catcattccc tctattactg cgaagaatcg
     1861 acatggctcc cgttgttcga aacgctgttc cgcacggtcc tcgccccgcg gggcgccatc
     1921 cacgccgtgc tcatggcgaa cacgagccgc gacacacaca ccacgacctg gctgtacaac
     1981 catttcgcgg ggaaattttt cggcattcgc aacaatcaaa gcctgccggt cctgaaacgg
     2041 gaactggagc gatcgcgggt ttttgacgat tcgcagatcc tcatctccac gagctccgtc
     2101 aattttttcg tgggcgattt cgaaaaattt atgaaggtcg tctggatgat cctgctctat
     2161 ccgaatgtcc atccgttcac cgaatcgcag aaggtggaga tcatcaggtt cgttatcgac
     2221 accttctggg cgccgaaacg gccgctcgtg caaacgcagc accacctcgt atgctaccgg
     2281 gggctcgact tcaaggggat gatatagccg ggggacgttg aggctttcgg acatgggagt
     2341 acaatcactc gccattgcat tcgtccttac gcacggatca cgggcgaaat gcgcatcgca
     2401 acatttcttc gtgttcatac ggagaattgg cgccgggcag cgaaatcgat catcatgacg
     2461 cggaggttcc tgcgatgatc gaaacgctcc tgccggcatt catcatggag agcgcggcgc
     2521 ggtacaatgc cgtcgtgatt ctcgccgccg tcgcgctctt cgcctgcctg ctcttcgcgg
     2581 gcgctgcggc gggcgtcttc ctcgcctttc gcatcgacac cgcgatgaag cgtcggggcc
     2641 gcaatctcga aatggcgctc ttcgccgtga tcctgcttct ggtccttgga gtcgcgctcg
     2701 cggccttcag cgtcgccgtg ctctttgcgg ttgcgcacct gcggtgcggc tgagggaccg
     2761 ttcgaatgcg cgcatgcccg ctttttcgct tgatgaaatg ccggcatcta attacaaggt
     2821 cgtacggact gtattgtaac aggtggtgtt gatgcgcggc acgaaacgac tgtcgcgggc
     2881 ggcctgcgcc gcaatcgcgc tcgctgtatg cggcgcgctt ctggccgcgt gccgcgagcg
     2941 tccgcacgaa accgtcgact ttgtgatggg gaccgtcgtc cggcagacgg tctacggccc
     3001 ctccgccgaa accgccgcgc gcgaggccgt cgaacgtctg cgggcgctcg aggcggcgct
     3061 gagctttcac gacgccgcga gcgacatttc gcggctcaac cgctcgtcgg gcgggccgca
     3121 ggtaacgctc gggccggagg cgatcgggct cttggagcgc tcgctcgagt tctcgcggat
     3181 aagcgacggc gccttcaata tcatggtggg cccgctggtg cgtcgctgga aggtgaccgc
     3241 agacaggccc gatgtgccgc cgcccgcgga gatcgcgtcg ctcgtgcggc tcgcctcgta
     3301 ccgcgatctc tcggtggacc gcgaccggcg gaccgcacgg ctcgcccggc gcggccaaat
     3361 ggccgatctg ggcgggatcg cgaagggtta cgcgagcgac gtgatcctcg cgctgtaccg
     3421 agcgcgcggg gtgcgctccg ccatcatcga catcgggggt aacatcggcc tcctgggttc
     3481 gcgccctgac ggtcgtccgt ggaacattgg cgttcaggac cccgggaggg agcggggcag
     3541 ctacatcggc tatctctcgc tcgcggacaa ttcggtgtcg acctcgggcg gatacgagcg
     3601 tttttttgtt gccggcggga ggcggtattc gcatatcatc gacccgcgca cgggataccc
     3661 ggcggactcg ggactgtcga gcgcgtccgt gatcgcgccg tccgccgagg agagcgacgc
     3721 cctctcgacc gccgtgttcg tgttgggtgc gtcgaagggg atcggcctga tacaacggcg
     3781 tcccggcgtc gaggccgtgt gcataacaac tgacggaacg gtgatcgtga ccgagggatt
     3841 gcggggaagg tttaccctca ccgcgccgga gggaacgtat cgcgtggaat atcgataccg
     3901 ggtcgggccg gatgcggccg gacccgcgcg gcagaggtga acgaatgggc aaggcgatga
     3961 aggcgtttcg gggcgggatc gcggccccgc acaacaagca cacggagaac tgcgcgaccg
     4021 aggccatggg cgtccccgcg cggatcatca tcccgatggt gcagcacatc ggcgcgccgt
     4081 gcgccccgct cgtccagaag ggcgatcgcg tcacggtggg ccagaagatc ggcgacaacg
     4141 acgccttcat ctcctcgccc atccatgcga gcgcgtcggg gaccgtggcc gacgtgcgtc
     4201 ccatcctcta cgccggcggc gtggaggtgg tatcggtcga gatcgagacc gatggcgcgc
     4261 aggagacctg tcccgacatt cgcccgcccg cgaccgaccg cgagtcgctc ataaaggcga
     4321 tccgcgaatc gggcatcgtg gggctcggcg gcgcgggctt ccccacggcg gtcaagctct
     4381 cgcccccggc cgacaagaaa atcgacatcc tcgtcttaaa cggcgccgag tgcgaaccct
     4441 acatcacctc ggactatcgc gagatgatgg agaaccccgg cggaatcatc gagggcgcgc
     4501 gcatcatcat ggagctcgcg aagatcccgc gggcgctcat cggcatcgag gcgaacaagc
     4561 cggaggcgat ccgcctcctc accgacacgg tcaaggacga tcccgccatc gacgtcgtca
     4621 cgctgcggac gcgctatccg cagggcggcg agaagcagct catctacgcg accacggggc
     4681 ggcgggtccc cgccgggggg cttccgtccg acatcggcgt gctggtgcag aacaccacga
     4741 ccgcgtcctt catcgcgggg ttcgtgaaaa cggggatgcc gctcatcaag aagaaggtga
     4801 cggtggacgg ctccgcgatg gcgacgccga agaacgtcga ggtgctcatc ggcacgccgc
     4861 tcaaggacgt ctttgaattc tgcggcggct tccgcgagga gcccgtgaaa atcatcatgg
     4921 gcggccccat gatgggcgtg gcgcaattct cgctcgacaa tacggtcatc aagcagacga
     4981 acgcgctttt agcgcttggc gcgcgcgagg tcgccctcaa gccggagatc gtgtgcatca
     5041 agtgcggccg ctgcgtctcc gcctgtccca tgggcctcct gccgctgtat ctgaacgcct
     5101 gcatggagcg ggggttgttc gagcggatgg ccgacgacta ccacgtgagc gactgcatcg
     5161 aatgcgggtg ctgctcgtac gtgtgcccgt cggcgcggcg gctggtgcag tcgatccgct
     5221 acggcaaggc cgagctgcga aaactggccg caaagaagaa atgacggcgc cgccgcgaac
     5281 cggcgcaagg gggactacac tgtggaactg aaaacgggca tactcatcgc gggctcgtcg
     5341 ccccacctca ggagcgatgc gacggcgcgc cggatcatgc tggacgtgct catcgcgctc
     5401 gttcccgcga ctgcggcggc ggtctacttc ttcggcgtgc gcgcggcgct ggtaatcgcc
     5461 gtgacgacgg ccgcgagcgt cctggcggag ttcgtatcga ataaaattat gaaaaaaccg
     5521 ataactatcg gtgacctgag cgcggccgtg acgggcctgc tccttgcgtt caatcttccg
     5581 ccgggaattc cgctgtgggt ggccgcgatc gggggcgtgt tcgcgattgt cgtggccaaa
     5641 caactcttcg gcgggctggg catgaacttc atcaatccgg cgctggccgc gcgggccttc
     5701 ctcatggcct cctggcccgt cttcatgacc acctggacga cgccgatggt ggacgccgtt
     5761 tcgtcggcca cgccgcttgc gatcatcaag agcgcggagg cggccggcgc gacgcccccg
     5821 tcgatcgcgc agcttttcct ggggaacgtg gccgggtgcc tcggcgagac ctcggcgctg
     5881 gccatcctcg tgggcgcggc ctatctcgtc gtccgccgcg tcataaccct ggagacgccg
     5941 ctggccttca tcggcacggt ggccgcgatg acctgggtgc tgggcggcaa ggggcttttt
     6001 tccggcgacg tgccctatca tctcttcgcc ggcgggctca tgctgggggc gttttttatg
     6061 gcgaccgact atccgacctc gcccatcaca tggaagggaa agatcatctt tggcgtgggg
     6121 tgcgggttgc tcaccagcgt catccggctc tacggcggct atccggaggg agtgtgctac
     6181 tcgatcctcc tgatgaacct cgcggtgccc atcatcgaca aattcacggt ccccaaaagc
     6241 ttcggaggtg cgtaagcgat gcgcgatata gtgaaaacga cgataccgct tctggcgctg
     6301 tgcctcgcgg tggggctgtg cctctcgtac gtgaacgcgc tcacgaaggg gcccatcgag
     6361 aagcgaaaga tcgaggacgc ggagaagaag aagaaagagg tgcttccgga ggcgcagacc
     6421 ttcgaggaga tcgccgactg gaagaccgcc gcgccggagg gccatgtgct caccggcgcc
     6481 tatcgcggca tgcgcgacgg gaaaacggtc ggctatgtct tttccgccgc gcccaagggc
     6541 tatgccggcg cgatgacggt cacgatcggg atcaagcgcg acggcacggt gggcgcggtg
     6601 agcctgggcg ataataagga aacgccgggc ctgggaacca aggcgggcga ggcgccgttc
     6661 atcacccagt ttgcgggaaa gacgatcgcg acgcccatcg agctcgtgaa gatgaccgcc
     6721 tccggcaacc aggtgcaggc gatcgccggc gcgaccatat cgtctcgcgc gatggtggtc
     6781 gccgtccagc acgccgcggc ggtcgcgcgc gaactactga aacgggaagg gggaaagcca
     6841 tgagcctgct cgcaatcatc aaaaacggaa tcgtggacga gaaccccacg acccggctcg
     6901 tgctgggcat gtgtcccacg cttgcggtca cgacctccgc gatcaacggt atcgggatgg
     6961 gcgtctcgac cctggcggtg ctgatgggat cgaacatcgt gatctccctg ctgcgctcgg
     7021 tgatccccga caaggtgcgc atcccctcct acatcgtggt gatcgcgggc ttcgtgacca
     7081 tcgtgcagat gaccatcaag gcattcgcgc cgtcggtgga ccaggcgctg ggcatcttca
     7141 tcccgctcat cgtggtcaac tgcatcatcc tggcgcgcgc ggaggccttc gccagcaaga
     7201 acggcgtcct cgcctcggcc gccgacgggc tgggcatggg tatcggcttc accatcatgc
     7261 tgctgatcat cggcggcgcg cgcgagatcc tgggcaacgg ctcgatcttc gggatcgccc
     7321 tctacggcca aacggccagc cccgcgctga tgatgatcct gccgccgggg ggcttcctcg
     7381 ctttcggggt gctcatcggc atcgtgaaca tgctgtcgga aaagaagatc acgcagatcg
     7441 gctgcgattc ctgcggcgcg ccgtgcaagc gtaacgcgga gaaccggggg taggcgccat
     7501 ggcggaacag atcatcacat cctttcttgc ggccgttctc atcgaaaact tcgtcctggt
     7561 gcgcttcctg gggatctgcc ccttcgtggg cgtgtcaaag cggctggaga cggccgtggg
     7621 catggggggc gcggtcatct tcgtgatggc gctcgcctcg gcgatcacct ggctcatcca
     7681 gcgcttcgtg ctcacggcct tccaggtcga atatctgcag acgatcgcct tcatcctggt
     7741 gatcgcctcg ctggtgcagt tcgtggagct gatcatcaag aaggtgagcc gggggctcta
     7801 cgattcgctc ggcgtgtacc tgccgctgat taccaccaac tgcgcgatcc tgggggcctg
     7861 catcatcaac atccagaagg ggttcgattt catcaatgcg gtcctgtacg ggaccttcgc
     7921 cgggatgggc ttcacgctgg cgatcgtcct gttcgcgggc atccgcgagc ggctggagct
     7981 cttttcggac gtccccgcga acatgaaggg gtttcccatc tccctggtcg cggcgtcgct
     8041 ggtgtcgctg gcctttttcg gcttccaggg cctcatcaaa taggaatggc ggggtttgat
     8101 catgaatgag attattattg cgacggttat cctgagcgcg ctggggcttt tcttcggcgt
     8161 ggggctgtcc atcgcctcga agatattcgc cgtgacggag gacgagcgcg tcgcgaagat
     8221 acgcgcgctc ctgcccggcg cgaactgcgg cgcctgcggg tatcccggct gcgacggcct
     8281 cgcggtcgcg ctggtcgagg ggaaggcgcg ggtgctcgac tgccccgtcg gcggcgagac
     8341 ggccggcgac gccatcgccg aggtgctggg gcttgccgtc gagaagggcg agaagatggt
     8401 cgcgcgggtg ctctgcaagg gcgactgcga cgtgagccgc gataagttca gctatgacgg
     8461 cctgcaggat tgcgtcgcgg cggcgcagat cttcggcggc tcgaaatcgt gccgcttcgg
     8521 ctgcctgggc tacgggaact gccagcgcgc ctgccccttc ggcgcgatcg acatgatcaa
     8581 gggcctggcg gtcgtcgatg aggcgcgctg caagtcgtgc ggcctctgcg tggcggcctg
     8641 ccccaaggcg ctcatcgaga tggcgcccat gacgggccgg cacttcgtgt tctgcaaatc
     8701 gaaggacaag ggcgctgcca cgaagaaggc ctgcgacgtg ggctgcatcg gctgcacgcg
     8761 ctgctttaaa acctgcaagt acggggcgat ccgcatggag gggccgctcg cgatcatcga
     8821 tccatgcgtt tgcacaaact gcggcgagtg cgtacccgtg tgccccacgc actccatcgt
     8881 gcgcatactc gcgccgggcg agacggcgcc gacggccgtt cccgtcggcg aggaggctac
     8941 ccccgcatca gccgagcaat cgccgtcttg agcgccgtcg ccgcctcggg gtggtacatg
     9001 atgagcacgt ccgccccggc gtaaaggagc gaggtggccg tggcgagctc ccagtaagcc
     9061 gcgcgtttgt ccagatcgcc ccatgacggg aacgatgccg cgtcggcctt gagctccttg
     9121 agtttggcgc actcctgtcc cggcgagacg atcatgggcg aggcaagcat cgcgtcgccc
     9181 cccaggccgg taagccggat gcgctccatg accgagtagg tgtactcgat gccgtagccc
     9241 attgcgccgg tcatcgggtc gatgacgatg cggtcggcct tgaagtccat gttggtcagc
     9301 aggatgttca tctgcttggc gatgttcacg tcgatggggc tctgcgcgac gagcgagtgc
     9361 ccgtaggcga tggccgctcc cgcgacggtg cgatagttgt cctgctccac ccagttgaac
     9421 agcaggttct ctcccgcgca gccgctcgcg atctccttga agacctcgtt gatccgctcg
     9481 tagttgttgt gcccggtgat gatgagcggg acgtccaccg ccgcgagcac ctccttcacg
     9541 agcgcgagcg attccgcggc cgagcggttt cccttctcgg ggtgcgtccc ctcaaggcgc
     9601 acgctgatga ggtccgcgcc gtatttctcc acgcaggcct tcgccatctc agcgggccgc
     9661 tccacgaggt cgccccagaa tttccgcagc accggcgggt acttctcgct cacgcggtcg
     9721 aagacctcca tcgcgacgag ggggcgattg ggcatcgctc cctcccagag gtggaagggc
     9781 atcgccgtcg caccgccgat ctcgagcgtc ctgccgcgcg tgccgccctc cgccttcgtc
     9841 gcgccaagct tcacgcggtg gacgggaatg gaggccggtt ccttctttac ggcaaaggcg
     9901 gtgattgcgc cgatgcgctc ggccgccgag ggaagattat ccttcggcga cgcgccggcc
     9961 gccgccggtc ccgtcccgtc cgcagcgcca tcagcccccc gtgttgatgc gggccgcgcg
    10021 cccaggaagc gctctcccag gacgtcgatg atctcgcgca cgatctcgtg ggtgaggttc
    10081 tgcttgaggt ccttgtggat ctccgcgatg aggtcgcttt tgagcttttc gatggcgtcc
    10141 gcgccgaggg tgatcgcgcc atcgtccctc ttcgcgccct cgacgggttt ctcggccggc
    10201 ttcgcttcga cagtcggcgc ggcggcgcga ccgcccggcg catcatgcga ttcggcatac
    10261 gtttccatgt tttccatgaa aagggccggg tgcttcacct tctccataaa tgttcggatc
    10321 tcgtgcggat ccgtcgcgac ggtctcgtcg gcgatcttgt cgaagaagtc cgggaagccc
    10381 tcgcgctcga agatgcgcgt gaggtcgttc ttcaggagct ccttgagctc cttgggcagc
    10441 cacacgagcc gccggtgccc gccctcggcg aagaggaact tgcgcgaggc gaggaacacc
    10501 ttgccgcagc ccatgaagcc gggcgtctgt gcgccgccgc ccacgttgcc cgcaagcgtg
    10561 gagaaggtca tgcccacggg cgtgtcgccc aggaactcgc ggttcacgcc catcacgccg
    10621 ttgcattcgg gcacgtaggc gaggatcacc tcgaagcagc cgcagctggt catggggcgg
    10681 tccatgatgg agtacaggca aaaggtctcg accgacttgt gcgattgcgg gtacacgtac
    10741 tcgtccacgc cggtgaagat ccccttcacg gggtccacgc aatcgccctt cttgacgggc
    10801 tggttcgggc ccgtctcgtc gatctcgtag gccgccttgc cgtccagcca gttgtacgcg
    10861 ccgcacaggc ccaggcgctc gggcgtgatc acgcacacgt ggttgggtgc gaaggactgg
    10921 cagaggaggc aggagtagaa ggtgtccacc gactcgtcgg tcatggactc caagcggcgg
    10981 ttgcgctcgt cgtatatctt gcgcgcgatg gccacgcgcc gctccacctc gtcgggatcg
    11041 gtgatgatgg tcacctttac cttgtcgacg atggcgggga agttcgataa aaacttcgcg
    11101 tgaatgatct cgccgtaatg gcgcaggcgc aggcccttct ggaagccgct cttggagatg
    11161 cgcgtccaca cgatgtcgcg ctggcccatg tgccagatcc cctccgcgcc gttcaccagg
    11221 tggtggatct gccgctccag gatggactcg aagtcgacct gcatcttccg gccggccgcc
    11281 tccacccaga tcgccagcgg cagcgcgctc ccgggctcca cctcgtcggc gtccttgccg
    11341 atgatctcga tcacgccgtc ctcgatctgg tccatctcga ccatctgcac gaactcgaag
    11401 gcggtggtga tgttgccgcc gaattcgacc tgcgtgtcct gcttgcggat gcgctcgccc
    11461 tcgaaggccg cgccgtaggg cacggggatg gggaccttcg ttaccttgat cttgcagccg
    11521 cgcacctcca gcgcgcgctc caccatgcgc tcgtgcggga cggacgacac gacgtgctcg
    11581 taggtgcaca cgcccgtcgg gaggatctgc gggatgtcgg tgtccgcgat cacgggaaag
    11641 ccgtagttga tggcgcccgc ggccatggcg tatttctcgg agtccacctc gcccagggcc
    11701 aggacgaagg cgaagatgcg gttcttgttg tatttcaaat tggccgcgaa gtcgccgggc
    11761 ttcacgccgc cgaatgagag cgcggcgcgg ctggcgaagc ccagcgggta gacgagcgcg
    11821 gagacgtcag gcccgaaggg gaccagtcgc gtctcccagc cgagctggac gccctcctcg
    11881 gcgagctgct cggcgaattg cttcccgttc gtcgatccgg ccatgaagac atagatgttc
    11941 ttctcttgga gctcgcgcgc gattctaacg gcgatctcgt tggtgggggc ggcgccggtc
    12001 acggccgcga agccgggcgc cgtgccgtcc acgaactcca caccccgctc gcgcatgatc
    12061 acgtcgttcg ccgcgcccag ccagatgccg tcgatgggat ttggccccac caggtacttg
    12121 cacgcctcga tcacctcgca ggcgaagagc gaggcgacgc cggcgtcgag cgtgttgccc
    12181 agatacggca gccaggtatt ttcggaggga cggcccggca ggagctcctt cgcatcgcgg
    12241 agcgcgcgcc ggagatcgcc cagcgtctcc atcttcgcgc cggtaaaaga atatatgatc
    12301 ggcaggaagt aggcggtgtc gggatagccg accttcacct cctcgcccat cttgtcgatg
    12361 gcctggtcga gcttcgcttc ggcgcgcgcg acccattcga tcgcgccgtc gatggcgctg
    12421 gcgcagatga tcttagacat cgagctccct ccggtctttc atgtcgtaga gcttgcgctc
    12481 cgctttcttg ttaatgccca gtttctcgcg ccgccggtcg atgatggcga ggatggcctc
    12541 ggccgcctcc agcgggtttt cgtgcaggtg aaacgacgcg ccgaaggcct cggcggagcc
    12601 gccgttcaga tagttcgtga cggtctcgga accggagatg tagaaaggat tcccgagcac
    12661 cacgtcgacg cccgaggcga cgaaatagca gccgatggaa acggccttct cgctcatcca
    12721 ctcgggggcg acgcccacca cgggaacgtc cgagagatcg tcgcccaggc cgccctcgcg
    12781 gaccacctcg gtcgcggcct ccagcaggcg ggagttgtcc acgcagcttc ccatgtggag
    12841 caccggcggc atgccgatcg cctcgcacac ctcgcgcagg cccgcaccgg cgtgttccag
    12901 cgccgcctcg ggtgtgagca taccgctctt ggccgaggcg atcgcggcgc aaccggtctt
    12961 gagcaccagc acgttgtttt tgagaagctc cttcgtgagg atgttgatgt aatgatcgag
    13021 ctgctgtttg ggattgttgc agcccacgat gcccgccaca cctctgatgc gcccttggat
    13081 gatggcgtcg ttgagcggcc ggaacgacgc gcggaagcgc ccgcccagca tgtacttgat
    13141 ggcctccacg ctgaatcctg ccacgagcgg cgattgcgcg gccgggataa agaccttctt
    13201 cggttcgcgc cgcttgaagt tggcgatggc gcggaagagg aggtcgcgcg cgctcgcata
    13261 ggcgtcgtcg ggattaaagg ccgcgtggtg cgcgcccacg gtcttggcga tatcgctggt
    13321 ggagacgatc tcggtgtgat acgacgcggc cacctcgggc aggcccggca tgcagcactg
    13381 cacgtcgatg atcatcatct ccacggcgcc ggtcacgatg gcgagctcct gctgcaggaa
    13441 gttccccgcg acggcgatgc cgtggcgcat gaggacctcg ttggccgtgc agcagatgcc
    13501 ggcgaggttc acgccctcgg ccccggcctc cttcgctcgc gccgtcacct cgggatcgcc
    13561 cgccgcggcg gcgagcatct cggagagcgc cggctcgtgg ccgtgcacca acacgttcac
    13621 cttgttcttc tcgagcacgc ccaagttcgc ggcgctcttg accggcgagg gcgagccgaa
    13681 gatgatgtcg gacacctggc tcgcgatgcg cgagccgccc cagccgtccg cgagcgcggt
    13741 gcggaaggag tgcaggagga tgttgtgcgg atcatggtcc acgcccatgt gtgtgcggtg
    13801 cattgattcg accaccatgc ggtcgatccc ctcgggcgcg atgcccgcgc gctcccaaac
    13861 ggcctgacgc ttcacgggcg cgagcttcag cgtctcgagg tgctcctcct gggccgtgaa
    13921 atcgcgcaga aagacatcgg cgagctgtag cgcgagctcc tgcgtggcga cgttctcggt
    13981 ctcgaggccg tattctcgcg caatgcggcg aagcgcgggc tcgtccttga tcacgtaacc
    14041 gccgcccttc ccctcggcga tctttttcag aagcagcacc aggtgccgcc cgtgatcgga
    14101 gtgcgcggcg gtccccccgg ccacctcgcg caggaagttt cgcgccacga tggtgtcggc
    14161 gtcggccccg caaacgcccc gcggcgcctt gggggtgatg cggcagggcc ccatgtggca
    14221 gatcttgcag cacacgccgc ttttcccgaa gccgcactgg ttcttctgct ggtccatgcg
    14281 gaagaagctc acggtcacgc agtcgcgttc cgctttttcc agcgtttcta tcgaggcgcg
    14341 gtcggtggcg cggtcctgag cggcccgtat ggttttatcc ttttccttgt ccatggtctc
    14401 acctgtgctg atgaccccct ccggccttcg accacctccc cctttataag ggggaggatg
    14461 agtatggggt cttcattgaa tcgtgtcagt tagacaaaac acgttgtatg aatgcatata
    14521 tctgcgacga cacattgttt tccgtcgata atcccgtgat cgaactcccc cgctccgagg
    14581 ccgctgcgat ttcatcgtcg tcgcgaagca gcatcacgtg atgtgcgcca atgccgtcaa
    14641 tcatcaacct cgcgttctcg atgagcgaat cacccttcat ccggttcacg ataatggcaa
    14701 gggacgtata gccgattccc atctcgccgg cgagctcgtg caggcgctgc acggtcttca
    14761 cgccattgat cgacggctcg gcgaccaaga tcaacaggtc caccgaggcc acgatgcgcc
    14821 gcgagaggtt ctcgagcccg gcctcgttgt cgatgacgac atacgggtag ctcgcggaaa
    14881 ccccggcgat cacgtcgcgg agcacgttgt tcgcgtaaca gtagcagccg ggtccctcgg
    14941 gccgccccat ggcgatgaga tcgaacccgt cggcctccac caggcactcg gaaatcctga
    15001 gctccaacag atcgcgcttg gcgatgcccg aggccatgcc cttcgcggcc tcttcgcgcg
    15061 cctcctcgcg gatggtcccg atggatttct ccacgcgcac acccaggacg gtatccaggc
    15121 acgagttcgg atcggcgtcc acggcgagca ccggcgtttt cccgaggcgg accagcgcat
    15181 caacgatgag cccggcgatg gtggtcttgc cgcacccgcc cttgccggtg atggcgatgg
    15241 tatatcccat ggcagtattc tagcgcgaaa caggggattt catgtaataa tctcttgatt
    15301 ggcccatcga cgatgagcat cgccacatcg ttatgccgcc gaccggcatg aacgacatac
    15361 gtattcgaag gacgtttttt cgcaattgtt tttgtggtga atttcaatcg tgccacattc
    15421 acgtatttct tcgcacttga atgctcgagc gcagcgaagc tgtacttccg aacctccttc
    15481 ctctttacca attcgttagg ggtacgggga gcgtcaagag gtggtagtga cacccggcct
    15541 ccccctagcg aagggggagg ccggtggggg taaaaccaga taaatgattg aaaattattc
    15601 ctttccgcat gagaatcacc catgctcgcc ggaaaccata tcagcgctgt ttcgctcgcc
    15661 tttctgggcg cctgcgcggg tactctcctc gtcggggtcc tgatatacgg gatcatctat
    15721 cacttcaccc ggagcccgct gcatgcgggt gtggcgctta tgggattgtt cggattcgtc
    15781 ttcgtggccg gcgagttggg cgtggccctg tgccatctgg ccaatgaccc gctcctgggg
    15841 cgccggatgc atcggatgca ggcgctcgcc gctgccttct tcatctttgg ggttccctat
    15901 gtcgcctctg gcctcacgga tctttcgcac gcatggcgca gcgcgaaccg ggtcgtttcg
    15961 tacgccagtc tcgccatcgt cgccgcgatc gtagtggtcg ccttcgcgat gccggatttc
    16021 ttcatctcgc aaacctcggg cggaatggaa tcgctgcatc gcctttcgca cgatgccgtc
    16081 cgcgggcgca ccgggccggt gtaccgcctg cgggacatcg tcattgccgc gctcatggga
    16141 tatgcgctcg ttatgttcgt cttcgacctc ctgtggcacc gtaactatcg gtatatctcg
    16201 cttcccctgg ccggcagcgt gacggcgatt acgagcgcct tcgtggacta tttctacatc
    16261 taccggggcg tcgatggccc gttcccggcg cgcgaatcga tatcatattt cagcgtgggc
    16321 atcacgatca tgattgtcct gtgcatggtg ggggtcgtgc tgcggttcat cgaccagacg
    16381 cgcgacatcg agaaggcgca gcgcatcgaa tcgcttggcc tgttcgccgg cggcatcgcc
    16441 cacgacttca acaatctgct cacggccgtg atcggcaaca tctcgctggc caggctcgac
    16501 gcgggcgccg gacgcgcggc tcaggcgctc gatcaggcgg agcaggcgtc atttcgcgcc
    16561 cgggacctca cgcgccagct gctcaccttc tcgcgcggcg gcgagccggt cacccgcagt
    16621 gtcccggtgc aggacatggc aaccgagacc gcgcatctgg tcctgggcgg ttcgcgcatt
    16681 gaatgcgtat tcaatatcgc gccggacgtg cgcaatgtta tcgccgatcc agggcaaatc
    16741 gggcaggtgt tgcagaatat catactcaac gcccgccagg cgatggccga ccgcggacgc
    16801 atcgacatca cgatcgcaaa cgaagatgcc gccgcgggaa ggcgccgcga gcggcacgcc
    16861 gggccatatg tcaggatcgc catcgccgat accggtccgg gcatccaccg acgcgacctc
    16921 gcccgtatct tcgatccgta ttacacgacc aagaccgccg gcaacggcct tgggctcgcg
    16981 atcgctcatt cgatcgtcca caagcatcac gggagcataa cggtcagctc ccgcgcgggc
    17041 gtaggcacga catttgtgat tctgctgccg gcgacggatg ccgccgagca cggtgcttcc
    17101 gatcatgcgc ccgtcctggc gacgcccggc gctcatggcg ccattttgct cgtggacgac
    17161 gaggaaagca tccgcgccat gggacggatg atgctggagc ggctcggcta ttccgcggtt
    17221 acggcgcgcg atggacgcga ggccgtcgag gcatatcggg ccgcaaacgc cggggaatcg
    17281 ccctttcgat gcgtcctgct cgacctcacc atgcccgacg gcatgagcgg ccgagaggtg
    17341 ctggccgaac tgcgcgcgat cgatcccggc gtgcgcgcga tcgtcatgag cgggtattcg
    17401 aacgatccgg cgatcgccgc ctatcgcgag tacggcttca gggcttcgct cgcgaaaccg
    17461 ttcggtatcg acgatcttac acgggccgtc gatggcgtga tggctgacgg agggcccact
    17521 gcatgaaccc aatactgcgg attttctgat ctgcggtcgc acatatgcac gaatcacgca
    17581 tggaggtaac acaatgaaaa acatgtggat catcccgctg tccgtaatgc taattattgg
    17641 cgtgaccatt ggcattgtgc actcgcaaaa ggcccggaag ccggtttcca tcgccgcgcc
    17701 gaagctcttc ggctccgagc cgctgacgaa gatcaaccat caggacgtgc cgaacaatgc
    17761 gatggtgctg agcgacggct cggtgctcat ctccgtcggc cagaccggca atcccaccga
    17821 ggcccagacc aagatggaat caatgatgaa gaacgagaag cgcatgcccg ccgttccaac
    17881 ctatgcctgc agtacgtttg ggtgggcctt tgcgatgaag gtcaatgcgc gcgaacagct
    17941 ggagtggatg caggtcatct accccgagag cgtccgaggc ctgtacgatc ctcagaacca
    18001 gggatggcaa accaaccagc tccatttcgc gactgccgcg ccgaccaggg acggaatcct
    18061 gttcggcgga acggctccgg tcatgaagcc ggacgattcc agccagctcc ccgtgctcat
    18121 taagtatcgc aacaacggca aaaaggcatg ggaaaaggta tatgccgagt ttgccgtcgc
    18181 ggatgagaaa ttcctgagaa gcggtttcat gaacgtcggt gagacgccgc aggggatcac
    18241 cgcaatcatc tttgtgaaaa aagactattt ctgggtaaaa atggacgcga acggcgccat
    18301 cctgccgggc tcgaagcagc cgttcgcctt cagggggtca tcggccctgg aggcccgttt
    18361 tgcgccggac ggcagcctgt acacggtcga tgccgacggc ggcgcgatca acgtctcccg
    18421 gcatgacgcg aataccggcg ccggcttgtg gcggaagaag gccaatgatt ccgggtcgtg
    18481 cggcctcatc gggaccacca acatcgtcgt gacgcgcgac ggcggcgttg ccgtctgcgg
    18541 ttatcgggaa tcaggcgaca ctcgggacgt catcgcagcg cggttcgatg cgaatgggaa
    18601 ccggcagtgg gtgttccagt atggcgcgcc gggcgcgtcc cactggccgc gcgggatctc
    18661 gattatgccg agcggcgata ttgtcgtggc ctcgtcctgc gatcagggca actcgcgcta
    18721 tacccggctg attctgctga acgcccaggg caaggaagta tgtgcaaata attattcggg
    18781 catctccggc aattcgtatt tcgccggcaa tttcctcatc gggtacggct gtcggaggag
    18841 cgaggacgtc tgcgtgaagt acatcccggc gatctgcaag taaccggcgg gcggtatccg
    18901 gatcggacgg cgccgggcga gtttactgac ctgtatacgc ccgcagccgc tcctccagcg
    18961 tgcccgtgtg cagctcgaag aggtggttgt cgtagtcgta gaagtagagc gatcgcccct
    19021 cgcccgcgat ccggctgcgg tcattgtcga tgtcgagccc gagcttcgtc acacgctcgc
    19081 gatatgttgc gaactcggca tcgtcgatgc gaaaggcgat gtgattgtat gtgcgcgccg
    19141 taagggccgg tccctccatc aaggcgatcc agacggaacc gacgagaaag aaccgttccc
    19201 gcgagtgcga aaacgtccgg tcgccgctgg agtagatctc ctcggcgtcg agcacggtcg
    19261 caagcatcgc ggatgatttc tcgagatcgc ggacgatgag cgtgatgtgg cttatccccc
    19321 ggaccatgcc ggatcagtag cgcgttcgca gctcgccggg aagcgccttc agcacctcga
    19381 acaccttcac ggcgtcggcc acgggaagag agcccgtgcc gcaggagggc gtgaccatcg
    19441 cgctggcgta aattttcgcg agatcgattc ccgccttctt cgcgaggtcg gcggcgaggg
    19501 gtccgaacct atccgcgagc gaggccgcgt cgttcgagcg gatcgcctcg gaggtgggca
    19561 cgatgcccca ggcgagcacg ccgccccttc cgatgaactg cttcatcgcg tcgggataga
    19621 gcgcgatcgt ctcgccgtag gcgtaggcgt cgaagttgat gatgtcgaag ccggcctcga
    19681 ccgggatgct ccattcggtg ttgccgcagc agtggattcc ggccagcgcc ccgtcggcgt
    19741 ggatggcctc cacctgctcc gcgaggaggg cgatcacgtc gtcgcgcttc acggcgatgt
    19801 aggtggacga cccgaaggcc gagaggatcg gctcgtccac gaagcagatc acctgctcgg
    19861 catagggcgc gaacttccgt atctgccagc ggctcttgag cgcgatggcc ttggcgatca
    19921 cgtcgcggaa ttcctcgttg tagtagagcg cgcgcttgtt ctcgtcggtc accgtgagcg
    19981 agaaggagca cggtccggtc acctgcacct tgacgaacgg gtgcttcttc ccggcggcct
    20041 gcaggcgctt ctcgagcgcg tagatcccct tcgagtgcgc ggggccgatc gccagcggac
    20101 cgaaatcgcc gccggactcc atgagggtca tgtagcgctc gtagaagtcc gcgaagtcct
    20161 cggagtagtc gcccgaggtg tcgaagtgca ggcgcgactt cccggtgtcg atgacggcgc
    20221 gcggcatccc ctcggcgtac tggatctcca tggactcgag cgccccggtc ttcgggagct
    20281 gcggccatac cggcgcgtcc ggcatcgacg cgaagatgat gtcgagcggc tgctccggat
    20341 tgtcgtgcgg aaagctgccg atggcggtgg cggtgaatgc attgggaaga cgcatgggat
    20401 tctcctcgga tactgctcta tcgttttgtt ggattgcctg tcattgcgag gagtcccgac
    20461 tggtcgggac gacgaagcaa tctgtgtcat gaaacatatg agcgaatgcc gcattcctgc
    20521 gcgatacacg aaacagatcg cttcgccatg cacgcgatga cgacggaaat cgaggatgct
    20581 cgaaatcgtg aaacacgaac atggtgcgaa cgatcatcat ggaaaatgat gattcgatac
    20641 cgcactgtca agaatatttt ccctcgagaa aatcgacaag cgcctcgagg cgtgcgcgca
    20701 tcccgtcgtc catgccgtcg atcacggcaa cgccgtcgcg gtcgcttgcg agcagccggt
    20761 ccgagaaggg aaacgatgcg gcgatgtcga gtccggggag cgactcgcgc aggtagcgct
    20821 cgtcgtcatc ggaacggacc ttgttgacga ccaccgcgat cctggcaagg ccgatctccg
    20881 cggccatcgc gacgatccgc ttcacggtct cgacggagcg catgcccggc tccacgacca
    20941 cgacgagcgc ggttacgccg cggacggtcg cgcgtcccag gtgctcgatc cccgcctcca
    21001 tgtccatgac gagcgtctcg tcgcggcgca acaccaggtc ctgcaccagg gcgcgcagga
    21061 gcgagctctc cgggcaggcg cagccggcgc cgccgccctt caccgcgccg agcaccagga
    21121 gcgatacgcc gcggtgcgcg taggcgtagt cgtcggcgat gtcggagacc gtcggattga
    21181 gcttgaagat ctgcccgtac tccccggcct tcgcgccggt gcgctcctcg acgagctcgc
    21241 gatgccgggc gatggtcacg atggacccct gcgcggcgcg ggggatcccc agcgcgtcgg
    21301 cgaggttcgc gtcgggatcg gcgtcgacgg caagcacgct cccgccgcga cgggcgatcg
    21361 tgagcgcaat gcccgccgca atggtcgatt ttccaacgcc gccctttccg ctcaccgcaa
    21421 tcttcatcat caccgccccc gccgtcgtgt acggatcata caggagaacc gcttcgtatt
    21481 cgaaacgagg atttaatact tgccaacaaa tcccccgccg gtaggctttc accgccgatg
    21541 cggtttttgc aaggcaaatt ttccgcggcg cgctccgccg cacggacggg cagagggcca
    21601 tgggcgttca ggacgggtat acgccacgcg aggcgctcga gatcagctcg agcctgttgt
    21661 atgtcgtgat gctgtgcgtg atcacctgcg tcgcatcgct ctttcaggtg ttcgcgcagg
    21721 tctcccccgc cggcatcgtg ttcccgttcg cgctcaatct cggcgcgatc gcgctcgcgt
    21781 cggttatcca ttcccggaag aaggcgcgcc gggacagcga gctcctccag tggggcgtcg
    21841 cgctgctgac gatcaccgcg ccggtcctgg cgaaatacaa ttacgcacgc accttcgact
    21901 ggacgccggc gggctggacc ttcgcggcgc aatcgtacaa ttcgtccatc ctgctggtgg
    21961 tgttcgtggt gctgctgcag ctcctgtaca accgccggct cttcatctgc tgcgcgctgt
    22021 acgccttcgc ccactggatc gcgttcttcg tgatcgccta tgcgaacggc gcggcgctct
    22081 acttctacgc cctcagcaat gacgcgccgc gcctggacgg cgtgatcgtc ctgcgcgagg
    22141 tgttttacat tttctgcgtc gtggtgatcg ccatcgtcgc gtaccgcaat atcgcatcgg
    22201 cgcccggcca cgatggggcg cgggaataat gtgcttgatc ttttcggcag gcgcgcgtcc
    22261 gattaatacc ggcaggagca cacgacatag aaaccgcgga tgcgcggggg ctgcgttccg
    22321 gttcgcccgc gcggggtaat ggtgtcacgg gcgttcttaa agaggtgggg tgatgaaaca
    22381 ttccgattat accgcgcgcc aggtgctcga gatcaagtcg agcttcatgt tttttatcct
    22441 cgtcatcgta ctcgcttttt tcggcaacgg cttccagatg ctcgcaaagg tgacgcttgc
    22501 cggcctcctc ccgtcgatca tgctgaatgt cagcataatc gtgatggcgt ttttcgtgta
    22561 tatgcggaag cgggcgctac ggtcggcggc cgtgttcgag tggatactcg gctttgtcgc
    22621 gacaaccggg atcatattcg cgaaatataa ctatgcgaag tcgtacgact ggtccacggt
    22681 cggttggacc tacgcggcgc aatcgtacaa tacatccatt tatctggcga ttttcctcgt
    22741 cctccttcaa ttgctgtaca atcggaagct gttcatcttc tatgcgatat gggggctggc
    22801 ccattgggcg ctcttcttcg cgctggccgt catgaacggc gcgctggtgc acccggaggc
    22861 gatcggtccc gacggcattc cgtttctcga cggggtgatc atgcttcggg agatcttttt
    22921 catcattgcg gtggggttca tctccttcgt ctgctaccgc aatatcccca tcacccagga
    22981 atacgacgag cgcaccacac ggcagcggga gctcatcgag aagcaggcgg ccgaacagcg
    23041 cgcgctggtg gttgacatca aggagaagat gaacatcctc ttcgggcagg tggacgagca
    23101 gaaccagctc gtcggcgact tcaacgagaa gatgcagagc cagtcggcca ccttcgagga
    23161 gatgtcggcc acgctggagg agctgctcgc ctcggcggag aacatccacg tctcctcggt
    23221 ggaccagatc gacggcaacg tgaagatgga gaccatcgtg agcgagttcc gctccatcaa
    23281 gcaggaaacc aagggcaacc tggctgcgac cttccaggac atcgaggcgg tggtgaaccg
    23341 caccacgcag gccaacgagc gcctgcagga cgtggagaag accatcggca agatcaaggc
    23401 gcaggccacg cgcatcgccg agaccgtcac cctcatcgtg gacatcgccg accgcgtgaa
    23461 cctgctctcg ctcaacgcct ccatcgaggc ggcgcgggcg ggcgaatacg gcaagggctt
    23521 cgccgtggtg gccgacgaga tcgggaagct ggcctaccag accgccgaga gcatcaagga
    23581 gatcgagcgc gtactgtccc tgagcgcgca gacgaccgaa gagggcgtgg gcgtcatcaa
    23641 ctccaccgcg gagatgatca agggcatgat catgaacatg ggcgcgagct ccaacaagat
    23701 caaggtgctg caggagagca tcttcatcga ggagaagtat atcaacatca tcatcgacca
    23761 gatgaccaag aacatacagc tggccaagaa catcggcatg ggaaccgacg aacagaagag
    23821 cgcgatcgag agctcggcgc gggccgtgga gcacgtcaac gagatcgtca tggcgatggc
    23881 gaaggagatc agcgagctcg cgctcacctc gcagaacatc ctcgacaacg cccgcgacct
    23941 gatggcgcgg gccgagacga tcacgtgagc cggaggcgcg cgcagcaatg aatttcgccg
    24001 tgttttttac ggtcgcgctg tccctctatg cggccgtgaa cgcatatatc ttcgtgcgcg
    24061 ggtggcgggc gctcgtcccc gcggtcgtct cgcgcccgtc ctttacggcc gccttcctgt
    24121 ttctctccct ttcgtacgtg gcgggccggt tcctcgagaa ggtggcgctg ggcatggtct
    24181 cggatttcct ggtgtggacc ggatcgctgt ggctggcctt gatgttctat ctcttcctgg
    24241 cggtcgtggc gatagacctc gcccggttgg cgaaccgcct gttcccgttt ttcccgcgag
    24301 cgatcaccgc cgatcccgcg cgcgcggccg ccgtcaccgc ggtgggcgtg tgtgtcgccg
    24361 ggacgctcat cgtcctgggc ggcttcatca acaccctcct tccccgcacg accgagctcg
    24421 tggtgccgat cgaccgtccg tcgtggaagg gccgcacgat gaccgcgctc ctggtctccg
    24481 acgtgcatct gggcacggtc atcggccgga tgcggctctc cgcgctcgtc gagcggatca
    24541 acgcctgcgc cccagacctg gtgctgctgg ccggcgacat actggacgag gacgtgggaa
    24601 cggtgatgcg caaggacctg ggggagatcc tgaaaaacgt ccgcgcgcgc cacggcatct
    24661 acgggatcac cggcaaccac gagttcatcg gcggcgtgga gcggaccgcg cgcttcctgc
    24721 aggacagcgg gatcgtgatg ctgcgcgatt gggcggtgaa gatcgacggc gcgctgtatc
    24781 tcgtggggcg cgaggaccgc agcgtcacgc gattcggcgg ccccgagcgc gcgccgctcg
    24841 acaggctcct cgcggggctg gaccgctcgc tgccggtgat cgtcatggac caccagccct
    24901 atgaccttaa gcatgcggcc gcacgcggtg cggatctgca cctgtccggc cacacccacg
    24961 cgggccaggt gtggccggcg agctgcatcg tgcgcgccat gtacgaggtc ccgagcggcg
    25021 cgaagcggat cggcgcaacg ctgttctatg tttcgacggg atacggcagc tgggggccgc
    25081 cggtgcgcat cggcaaccgg ccggagatcg tcgttgtgcg cctcacgggg ccgggcgcgg
    25141 ggcaaaaacc ggtctgtccg taaaatatac tttacagaaa aacgccatgc cctgatgtag
    25201 ttcccggccc gcatagagcg gggatcccgg cggaaccaat cgacaccttg aaaacagaaa
    25261 ccagacagcg cgcggagcgt tccatgaaga ttgtcaccaa gaaggtcgag aacgtcatta
    25321 tcgtttatcc cccctcctgg ctcgacctgg acacctcggc ccagttcagg agccgcatcg
    25381 ccaagctcat cgatgccgag gacgagtgcc acctgctgct caacttcaag gacgtgaagc
    25441 acatcaacag cgccaacctg agcgagctca tcaaggcgaa aaagaagctc gagaaatcga
    25501 aacgcgccat gaagatgtgc ggcgtgcacg acaaggtcaa ggacgtcttc gaggtggtca
    25561 acttcaacac cttcttcgag atattcgaca ccgaggacga ggccctcttc tccttcaaga
    25621 attaatgccg cgcgttttca tcatcgccga catcgagggc agctccggct gctggaacca
    25681 cgccggatcg gccttcggga ccgacgagtg ggcgcgcgcc tgcgtgggga tgacgaagga
    25741 cgtcgacgcc gtggcccgcg ccctcttcgc ggcgggcgtt tcctccgtga ccgtgaagga
    25801 tttccaccgg accgggtaca acctgctgcc gcgccgcatc gaccggcggg cgcggctcat
    25861 catgggctat cggcggggag gagtccccgg catcggcgat cccggcggcc cgggcctgct
    25921 gatgatgatc ggcatgcacg cgccctccgg caccgatggt tttatcgccc acaccatgac
    25981 ctcccgcatc gcccgcctgg aggtgaacgg ggcgctcatg tccgaggcgc agctcttctc
    26041 ggcctcgctc gcgccctacg aaatccgccc ggtattcttt tccggctgtc ccgtcgcctg
    26101 cgcgcaggcg caatcggtcc tgcgaccgat aaccatccat tccatcgaca aaagcgccgg
    26161 tcccgatcgt ttcgatgccg atatgtggcg cacagaactc gcagcggcgg ccgtgagggc
    26221 tcttggtaat gacgcgaccg tcccctatcg gccggagggg ccgttcgatg cgcgcatcgt
    26281 catgcgggac ggcgaggagg ccgccaaaaa gcttgcctcc cgctgggggc tcgatcatga
    26341 gggacccgtc atccgcgtac aagcgataac tatccacgac ctctatatgg ccctcattcg
    26401 aatgtgctac ctgaccccgt tcatcgagcg gattttgccg gcggggctgc ccctctacaa
    26461 ttgtgcgggc tgggccgggc aacagtgggt gcggtggagg acgcgcgcgg aggggtaaag
    26521 aattcccgcg gcggtgccga acctgtatac gatacctatc ccagactggg aggctgcatg
    26581 aacgaccatc tcgtcattaa cgcgctggtg accgccgcga aacaggacct gtcgcgcgga
    26641 atcaagcggg aagacctgct gcaatcgctc gccaatgtcg taagcggcga catctaccgg
    26701 aaaatcaagg accgcatcta ggcggccgct acccctcgac ctccgacagc agcgcgtcga
    26761 agtgcgccga cgcctgctcg agcgccaggg tgctctcctc gagctcgtcg aagcgcgcgt
    26821 cgatctcggc gcggcagcga tggatgagct gcgagagctc cgacacgcgc ttcccgtttt
    26881 tctcctgcga cgcggtcacg atctcgcccg tgtgcgattc gagctccttc tcgagccgct
    26941 ggatggcccc ttcgatctcc gagaccttcg tttcgagcgg cttgagcgcc cgggcgcgct
    27001 cggcgacgat tttcgagcgg atcttgcgcg cctcggcctt tccgatcttc cggccctcgc
    27061 cggcgggctt cgacggcgcg tcctggcgga ggaccgcctc ctcgtccccc cagccgccct
    27121 tttcgagaaa gcgctgatag ccgccctcga aggcgcggac cgtgtcgttg tggaacacga
    27181 tgagacggtc cgcgagggcg tttaagtaca tctcgttgtg cgtgaccatg atcaccgcgc
    27241 cgtcgaagtt gtcgatggcc gcaagcagcg cgtcgcacga ttccatgtcg aggtggttgg
    27301 tgggctcgtc caggagcagc aggttcaccg gcgcggcgat gatgcggccg agcagcacgc
    27361 ggctcttctc gccgccggag agcaccgcga tctttttcag ggcgtcgtcg ccctcgaaca
    27421 tcatggaacc gcagatggtc cacgcgcgcg tgcggtcgag gccggggttg gcgttcatca
    27481 cctcgtcgag caccgagcgc gagtccttga ggacctgcac attgctctgc tcgtagtagc
    27541 ccatcaccgc cttggggttg atgctgacgc gtcccgcgtc cggcgccagc gcccccgcga
    27601 tcagcttgag gagcgtagtc ttcccgcggc cgttctttcc cactacgcag atccggtcgc
    27661 gcgcgcggat gctcatcgag agtcccgtga tgagcggccg gtccgtctcg tagccgaagg
    27721 agaggtcctc gatgttcacg atgtactgcg gcgtgaacgg aacgccgatg aaggagaaat
    27781 cgagcgtctt gatgttctcc agcttgtccc gctggcccat cttctccagc atcttcacgc
    27841 gcgactgcac cagtcccccc agccgcgcct tggcgcggaa gcgcgtaacg aagagctcga
    27901 tctccttgcg ccgctgctcg tcgttcgcgc gcgtcttttc gtatatctcc tcctccatgg
    27961 cgatctggga ataaagcttg tcggtgccgc cggcgacctt ccggagcttt ctgcggtgga
    28021 tgcccatcgt gtgcgtgacc acgttgtcca tgaaggtgcg gtcgtgggtg atgaggacga
    28081 gctcgccctt ccacgacgtg aggaagcgct cgagccagcg gatggaggtg atgtccaggt
    28141 agttgttggg ctcgtccaaa agcagcatgt ccggctcggc gatgagcacc ttcgcgaggt
    28201 tgaggcggat ctggtagccg ccggagagct cgcggggatg gcagcgcatc tcgcgctcgc
    28261 cgaagccgag gcccgagagg accttctcca cgcgccaggt ctcgccgcgc tcctcgtcgg
    28321 gcagcgaaag cgagccctcg gcgacggcgg tctcgcgcga aaaggcgagg tgctgctgga
    28381 ggtatcccat gcggtagttg cgcggtatgg agacgctccc ctcgtcgggt ccctcctcgc
    28441 cggtgatgat ccggaagagc gtggtcttgc cgtgcccgtt gcgccccacc aggccgatgc
    28501 gctctcgcgg gttcacgcga aagctcacgg cgtcaaataa cagctggcgc ccgtagctct
    28561 ttgaaaggtt ctcgacgaag atcatggcga taatatctcc ctggcatatg caagaacacc
    28621 catatccttg ggctcggcgg tttgacgcta cttggaaggg ttattgcagt ctgtcaattt
    28681 tttatccgtt accaatactg tcccgcaggc gaatactata cattaaaaga tggggcaata
    28741 atttaaactt aaataacacg aatgtgcttt ttttatcatt actgaaggcc tatcaaagca
    28801 ttaattgttg accacccgta tatcggatta ggtacagtgt ggatcataag gcttaaggcc
    28861 attacacagg agggtttggt tatgaagaaa aagctgttgg tcattggact tatcgtgtcg
    28921 ctggtcgttc cggtcttgta tgcaacggac gcggtcgccg agtacaaatt tacagtcaaa
    28981 aacagctcca agtcgaagat tgtcggtctc ttcgcacgcg aagcggggaa gaaggagtgg
    29041 ggcgcattcg atgttgggag cgggatcgcg tccggcaaaa ccgagaccat gcactgggac
    29101 gcatccacga acagctccgg gtgcgtatgg gagatcatgg caaagtacgc ggacggctct
    29161 tcatcggcgc cggcgaagtt cgatttttgc aaggaaaccg atatagagtt taccgactga
    29221 ttatttcgga ccgtccgtga cggccgtagt gatcatcacg tgacgaaact gccgatataa
    29281 aaaatcgtcc ccgtacgccg gggacgattt tttttctcaa tgcaatttca ttcccgttgc
    29341 gtccgttcct cattgccaga tatccatgtt caatgggcgg atcctgttcg ccgtaccgtt
    29401 cgcgtcgccg cggcagggat ggtgtcatta cccaacggtg aatgcgtttg cgcccctcgc
    29461 gcggagcatc acatggcaca ggccgtaaaa tatcgcaggc gaataatcat ccgc
//
LOCUS       DAORHF010000015        29127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_961_length_29127_cov_5.168100, whole genome shotgun sequence.
ACCESSION   DAORHF010000015 DAORHF010000000
VERSION     DAORHF010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 29127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29127
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_961_length_29127_cov_5.168100"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..768
                     /locus_tag="PKJ16_02500"
     CDS             88..768
                     /locus_tag="PKJ16_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HNV45882.1"
                     /translation="MCGRFSLTGGLEAIRRLIEDLESATELAPRYNIAPTQGIAILIN
                     DGTRLVRLARWGLVPAWARDEGMGERLFNARAETLAEKPSFKAQFRAKRCLVFADGFY
                     EWRKTPGSAKKTPYYIRLIARESFAFAGLWDLWTDRTDASRRLLSATIITTSPNELVA
                     PIHDRMPVILPRETVWQWLAPGDTQLAALQNLLAPYPPEKMEAFPVSLAVNNPGNESP
                     DCVAPLSS"
     gene            complement(1019..1399)
                     /locus_tag="PKJ16_02505"
     CDS             complement(1019..1399)
                     /locus_tag="PKJ16_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45883.1"
                     /translation="MKRFALCAIIASFALMMAPYIAGAQGDAEKTATENGLTVTVKKM
                     ADEIKGFKTFEVVAENTSGAKKTLNGRICLEDQTRKQPPCGSGECTIYMELGAGEKKT
                     MKKDCKEASRSNVWSVKIVKVYDF"
     gene            complement(1419..1583)
                     /locus_tag="PKJ16_02510"
     CDS             complement(1419..1583)
                     /locus_tag="PKJ16_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45884.1"
                     /translation="MKIDDCIKCKHHMDHVADQVLCRFWGETEHRVIERGIKDGIVYV
                     RLCPLEMKKK"
     gene            complement(1654..2598)
                     /locus_tag="PKJ16_02515"
     CDS             complement(1654..2598)
                     /locus_tag="PKJ16_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV45885.1"
                     /translation="MELRALQEALEEFIFVAQAHTLGVIERLHREPMSAGELARAMGF
                     DLRCASALLEGLAEMGYLAKRDQHYVVPADVAARLVDSQGDQYEGDFWRFLYYLLDPW
                     RTLPAVLATGRPDTESYRNFSIGDFIRAMNSPWKKRAVPEIVDRCLARHPDARSVIDV
                     GGAPGAVAREFAARGLQVCIYDLPESNAVLGRELAAVPGISVADGDATKEFPDGVFDI
                     AFLGNICHGQSVDDNARMIRMARDRIAAGGIIVIFDNIRGESYLGARLAMHMITQSPG
                     GNVYTRDEYFGWLADAGFQSPAVEHLSDRAWQLIIARK"
     gene            complement(2682..3143)
                     /locus_tag="PKJ16_02520"
     CDS             complement(2682..3143)
                     /locus_tag="PKJ16_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HNV45886.1"
                     /translation="MPGKKLKDFLDQNDILYTSIMHSPAYTAQGIAHSAHISGKEIAK
                     TVIVKVDGAFAMAVMPANRKLVLEILKDKLGAKEAALATESEFANLFPDCEPGAMPPF
                     GNLYGMAVYSCAELERDEEIAFNAGTHSELVRMAYKDFRRLVNPKVVDFTR"
     gene            complement(3323..4570)
                     /locus_tag="PKJ16_02525"
     CDS             complement(3323..4570)
                     /locus_tag="PKJ16_02525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV45887.1"
                     /translation="MFDFILTDAQKKLRDEAREFTRWVPREMILDMDAEKLHFPHEYL
                     KECGRRNLLGIRISKELGGRGLGWVEDGIVAEEIGVASYSLACLWGVGADIVCEAVAL
                     FGSDELKQAIVVPLLRGDVYAAECLTEPRGGSDFFGATTVARKDGGDWIINGQKRFIV
                     GAEGADWFMTYAVTDPGAPAHKRLTCFLIPRDAGVETKYLYGLMGVRGGGAGRVIFND
                     VRVPERYALNGINGGFDVFVKNMIPERLGTALMTIGSVRPAVEIATRYTSMRKAFGQP
                     IMNFQAVGFKVADSVMMLDALRAVGYSAALAADSKTAHAGRVRRMASQAKKFITEGAW
                     QIANNCMQVMGGIGYTSVYPLERIVRDIRLASIWVGTNEIQQSIIQTEWYKEYFKVLS
                     KEPVRDVEQDALGADQVEEKVYE"
     gene            complement(4598..4987)
                     /locus_tag="PKJ16_02530"
     CDS             complement(4598..4987)
                     /locus_tag="PKJ16_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014813159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV45888.1"
                     /translation="MPEERFTISELAEELDINPSTIRFYEQKGLLLPERTRGNQRVYT
                     RQDRGRLKLILRGKRFGATLDQIAEMIGMADEAINEMGQIDTSLYYIDEKMKEVARQR
                     SELDLFQADLEALKRKLNKRRRELMKK"
     gene            complement(5301..6485)
                     /locus_tag="PKJ16_02535"
     CDS             complement(5301..6485)
                     /locus_tag="PKJ16_02535"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNV45889.1"
                     /translation="MNRYTMLAAVLAALISLIGAPGSAHAAQRMVVTFTGDIMMHTAV
                     KESAAQNNRPGEGGAGSANNGGFDFLFDRIRPHLADSDVVLGNMEFPVSPPFRHGKGV
                     IFNCAPEIIPAMHAAGFTLFTLANNHILDQGNRGAIDTMDYLQRYAASFIGVNRHEGT
                     ARRGIVVQIHGIRVGFLAYTGILNYGFPVRPRGLHVNWLYWRNKTAADIRTMRARCDF
                     VVVVSHAGKEYKIRPEAEDMRAYRDSCEAGADLVIGHHPHVLQPVEAYTAADGRSCRI
                     FYSLGNFISNQRFKYHDHLRTLPYTTQETVLVRLVLERAWRGGLAARYEVLPLWTENE
                     IYREDGRERRRIQVWRMNERIEALERDGATGRAAYLRQRLDDIPKILFPESRPADVTY
                     VR"
     gene            complement(6490..8733)
                     /locus_tag="PKJ16_02540"
     CDS             complement(6490..8733)
                     /locus_tag="PKJ16_02540"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45890.1"
                     /translation="MRRAPKQIVAAIAGIVAWLVPLALVALPVRPMAGDVHYRDGFSP
                     SWTTAAPADDPAWRLVPADLRHCTAVRLTGGGVARYPSRTAFTPWKFPPVEFTLRSSF
                     ELPAGATGGGRILGLYISQIAENWEVFVNGALVRSEMHRGPDGAIAQHRDLRFVRFPL
                     HPGALREGRNELAVRIVGDPTFIDTGVFRRSQMVVDDYQAILKRHAETVYLSLITLYL
                     FVGIYHLFLFLMRREERFNLFFGLFTCMLFVYLYSRTFSAQELIPDTNILRRIEFVSL
                     FTLFPLVGAFLDLVLRGRVCRFTVGNAIASGVLVCGVVPFSLAFAVDVLRVWQFTAPV
                     PLAVILYRMVRSLVDMVREQRDRDGARGGTGAAFLRSLVRTVPGNLFLGGMIMIAFTG
                     FDIVDAVFFNRNVILVRYGFFVFVIGITIVLANRFRQVHERVEELNRDLGQKLDELGE
                     ASRAISLSEEKYKHLVEDTRDVIFTLDTALQVETINRAVYDHLRIEPSQLIGAPFISL
                     IYAGTEDTALSRQIAQEKLAELFHANEPVRFAAALNSAIGQEPKEMMLRFEMITIGGK
                     NQILGKATGIEEDFLLRHFVAERQRYEIGNYITSADELSMRLTRNCVRFLDARNVKYL
                     RIALREMILNAIEHGNLCITFNEKTEAIANDCYLQFIAQRRADPRYRDRTVVVEYSLN
                     GRRVAYRITDQGPGFDHAGFMARMREVNENLMVHGRGIMMAKNIFTRVRFNQAGNRVY
                     LVKTFQE"
     gene            complement(8740..9759)
                     /locus_tag="PKJ16_02545"
     CDS             complement(8740..9759)
                     /locus_tag="PKJ16_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNV45891.1"
                     /translation="MIRISMRAALAAVVSILALADVTLADQFAVARCDAMRKAHSIVA
                     RQKEVYAFCKPCGDTAAKRVVVSAPRVDPENAAPYENCTLRIGGREVDLAYVYVLHNG
                     RLENLAFLAGLAPVDVPRSLALAGGPSGEDAAGPQRPDTRPDAPTPGSADAPYLTQLE
                     REVVDELNRVRTDPAGYAAHLEGMLKYFRGRRYERPGAITLLTKEGAAAVQETIDFLK
                     SAKPLPPFVPSPGMSRAAKDQVNDTGPKGLTGHQGTDGSSVGSRLNRYGTWRRSCGEN
                     ISYGKNTAREIVIQLLVDDGVPSRGHRKNIFSPKFLRVGVGVGPHRTYRHMCVNTYAG
                     EYVEK"
     gene            10098..10700
                     /locus_tag="PKJ16_02550"
     CDS             10098..10700
                     /locus_tag="PKJ16_02550"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV45892.1"
                     /translation="MAAKLIIMTAVSILIAIAAVIAYRRYDIPLAERYRRIGKKKSWK
                     AVTYLGYSAVYIAPAAIAWLACTYYRPDPALAARALFVGVTVSASGIINALVKSVFGR
                     YRPYVWFDDKLFGFKFFQHKYGFTSFPSGHSNTSFCAALVFSYMIPYGAPAFFVLAGF
                     IAFSRVAINKHYLSDVLAGSGLGLVSAFAAYELYFRDLLA"
     gene            complement(10676..11566)
                     /locus_tag="PKJ16_02555"
     CDS             complement(10676..11566)
                     /locus_tag="PKJ16_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV45893.1"
                     /translation="MRRREPATRIDGVVFAGGGNRCIWQVGFWETAAPALGLRPKAVS
                     AVSAGAAMACMIFSGKTETALEYFTEATARNPKNFYPRNIVGSEPCLPHYGMYRDALT
                     HCLDEAALTRLHRHAINILIARPPRWLGPVSGTFVGLGAYSLEKKVSFPVHSSWARRI
                     GFRGEAVPVRSCATPSDLAELILQSSCTPPFVPVMRRDGRPVLDGGLVDNVPVRALDP
                     VDGTALVLLTRVYPAERLPRLPGLVYVQPSEPIRISKWDYTDPHGLREAYDLGRRDGE
                     RFVSEWAPRGAKAHASRSRK"
     gene            complement(11572..12588)
                     /locus_tag="PKJ16_02560"
     CDS             complement(11572..12588)
                     /locus_tag="PKJ16_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HNV45894.1"
                     /translation="MKQHVRDIFYGVIVGVANIIPGVSGGTMALVLGFYERLIGGIHN
                     ISGATVLSFFKALTLRRERIDAFKAEFDRIEGPFLLKLAIGAGISIGSLAKIMSYLLV
                     KQHDPTYGFFFGLVLVSAFTPWSLIKKKTLPTILAFLIAVAGIIAVSESMSREEIIQK
                     AEARQRIALQATESGAPAGAGVGVARLGFFFAAGAISISAMILPGISGSFVLLLMGGY
                     FDILRAISSLDLLILGVFAAGCLGGIVVFTRILNYLLGRFYDPTLGFLLGLVVGSLWV
                     IWPFKESMQVGAETVYLANRLPASVGANELITLAAFAAGCVIVAVMIYIERRGATENA
                     GGSM"
     gene            12831..14186
                     /gene="trkA"
                     /locus_tag="PKJ16_02565"
     CDS             12831..14186
                     /gene="trkA"
                     /locus_tag="PKJ16_02565"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HNV45895.1"
                     /translation="MNVVILGAGLVGFQLADELIREGNDVVIIEKNPDRARYVAGHLD
                     CMVINDEGTRLSTFKKARLARADFFISVTNVDEVNMISCGIVSGEYPDTVRIARVRNA
                     DYSRAGILSKSFLGIDFVVNPEVETARNIANAVALGADSDVMLFENSDMQIRNLVVKP
                     TSFFRGRTMKDIKKTLDEPFLVAGIARGDDFIIPSGDTAVLEHDNIYLLASKNTLTRI
                     FIKAGKKKEKIDRLVIVGGGKIGQLACQHLIRTGRRITFVESDYEICKALSSRFKRAL
                     VLHSDISDEDIFEQEKLSEYDLIVTTTNNEELNILTSVYAKSLGIKKAMALVSKASYM
                     PIAARLNIDSIINPKHSTVDAILKFLRRGDIRSVHSLFGGKAEVIEFIASETCTLAGK
                     RLMDIAMPHDSLILTILREGERDIIPGGDSVIRTNDRIVVISSKESVPEVDRFFSGTD
                     R"
     gene            14190..15641
                     /locus_tag="PKJ16_02570"
     CDS             14190..15641
                     /locus_tag="PKJ16_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HNV45896.1"
                     /translation="MNVLLVVKILAFLMLLYSLFMLLPTGVAAYYREYAALKTFVGTV
                     LAVVSGAGLLYVLLRRVKSASLTMRDGFFLVTVTWLLASVVGAVPFVVSGAIPSPADA
                     FFETISGLSTTGASILTDVEALPKSILFWRSLNHWIGGMGIVVLAVAILPLLGIGGMQ
                     LIQAEAPGPTVDKITPRIAETAKRLWLIYAGFSVVQTALLMAGGMGWFDSLIHTFGTL
                     ATGGFSSKNLSAGHYNSAYFDGVITLFMVLAGINFTLHYRLLTGRMGSLWRNTELKAY
                     LAIFAASTLFIAFGLLGNTYATFGESFRYASFQAASILTTTGFATADYEKWPLACQVT
                     LFLLMFVGGSFGSTGGGIKVFRIVALLKQAINEMRYLLHPRGVFTLRMNGEPVGKALV
                     FSISGFFFLYVIMLLITSAVVASSGQDLLTSFTAALATLGNIGPGFGAVGPADNFASL
                     PDYVKWFLSFAMLVGRLELYTVLVLFLPAFWRN"
     gene            complement(15650..16678)
                     /locus_tag="PKJ16_02575"
     CDS             complement(15650..16678)
                     /locus_tag="PKJ16_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV45897.1"
                     /translation="MFELTLIITLFTALSFLCSLLEAVILSVGRPYIQTLIDAKKKSG
                     AILQRMKDDIDEPIAAILTLNTIAHTMGAAVSGAIAVEIFGSKWMGAFSAVLTVIILV
                     LSEIIPKTIGARYWKRLSPVSAYLLRIMVTLLKPIIVPMNLLSRLIARGNHSDQVSKD
                     EVFNFAKIGFRQGAIDSGEFVIIENVLKLKKVRVQDIMTPRTVVVSLDPDARVTHVAD
                     TATDLSFSRLPLYDSRSNTVHGVVLRRDIMNCLAEKKIKVSLRELSRKPEYVPGGMSV
                     LALLSWFTKTGVHMGFVVNEHGDYIGVVTIEDAIESLLGVEIVDEFDPAVDMQAVARK
                     KMRRNKRI"
     gene            complement(16871..17872)
                     /locus_tag="PKJ16_02580"
     CDS             complement(16871..17872)
                     /locus_tag="PKJ16_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013714368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HNV45898.1"
                     /translation="MANRNTSTDHIVDIHEPVASIARTDYALLRDDLTIGRALDDIRS
                     RGLGERILYFYVVDADERLVGVLPTRRLLVSPLDQRLSDIMIADVITIRSDATIIEAH
                     QRLAENKLLALPIVDDDARVIGLVDVSMLTGVSFDINERDRVEELFELIGFRVMQVCD
                     ASPVRAFRYRFPWLTATIGSGLLCAMLAGVFEATLSTSIILAFFLTLVLGLGESVSMQ
                     SMTMTIQALRAMRPNLDWYMSAFWRELRSAFLLGAGSGTIVGAFIWLWHGKGLAALSV
                     GGSILLVIAAGCLYGLSIPTLLHRLRLDPKIAAGPLTLAITDISTILFYLGLATVVL"
     gene            complement(17945..18835)
                     /locus_tag="PKJ16_02585"
     CDS             complement(17945..18835)
                     /locus_tag="PKJ16_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV45899.1"
                     /translation="MMIAPPKKILLATDLNARSDRALDRSIALMRHYQARLVLLHVLE
                     ANAEPSGARRIPFFPVNRLVDPRIDGARRQLLDYLGEAGTSAQIRLEEGAPHRVIMRV
                     AREEGCDLIVTGVARNEALGRFSLGKTTDRILRAAEAPVLVVTERVRAPYRNVVALTD
                     LCAASKRAVETAAAFFPDQSISALYAFAAARATAVGERDRYIEQMRAVAERDLREFIA
                     GADLSPRQRDRMAQLVEFGDELPLLRELARLSSVDVVVVGATRRMPLLDAVFGGRVTR
                     IISSVPCDVLIVPGVRSFAC"
     gene            19022..19426
                     /locus_tag="PKJ16_02590"
     CDS             19022..19426
                     /locus_tag="PKJ16_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HNV45900.1"
                     /translation="MKTSIWNAINNPRSTYYIILIYLALSVLFSLCYWFIAPRIEGVQ
                     SLMYNMGGQSLVPVHGYFDAYYYSITTQTTVGHGDIVPATRGGKIVTALQVVVGYFYL
                     AFTISFFTCKSLVQSETFKAFFRNYEDDIASR"
     gene            19444..21291
                     /locus_tag="PKJ16_02595"
     CDS             19444..21291
                     /locus_tag="PKJ16_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV45901.1"
                     /translation="MSTSPAIRRIIQSLIVITVVSAVAWAADPGGDGHSSVVRSVGIA
                     MLAATVLAYFGNLIKQPLLLAYLAAGVVIGPSIGLGLIKNVHDIETISHFGLILLLFL
                     IGLEIDVKKLRESGKSLIIGGITQFPLTVAIGIGFFMLLGYTLGGGKYDLIYLAACCG
                     LSSTAIVVKLLYGKFELDTLAGRITLGILVFQDIWAILLLGIQPNLANPDVAQILLSF
                     GKGFLLVAVSMLISKFVLPRVFKSVAKVPELVLVSSLGWCFLVCGGAAYLNLSLEMGA
                     LIAGVAISTFPYNLDVIAKVVNIRDFFVTLFFVSLGVQIPNPLANTSILAVAAIVSIF
                     LVAARFAAIFPALYALNNGLRVSLLTPINLSQISEFSLVIASLGVAAGHIARDTLSII
                     IFVFVITSVSSTYMIKYSDAIQKTLARALRAIGLREIQSAAQDDDSAPGKEIALLGFH
                     RTASSLLHEIMDANSAATGGHTLADKLVVVDFNPEIHESLQSLGIKVVYGDIGHLDTL
                     HHAGIHDVRIVISTIPDTMLVGTDNLTIIRAMRDICPHAKIIVTAESTERALKMYAEG
                     ADYVFLPRILSAQRLMTVIGALLGDRPGELESVAAKEIEGLTERQEIIR"
     gene            21436..21969
                     /locus_tag="PKJ16_02600"
     CDS             21436..21969
                     /locus_tag="PKJ16_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45902.1"
                     /translation="MIRSAVLRAAVVILLAAPCALGAQEKKEEPNLRKIADDTKFQNA
                     LTFISLKKYDRALVELAEYLEVFVNGSHRREAYARTGDIHFERFDYRLAIRAYRTLSE
                     EFSGSPEGIEALYRIGLCYRKMGRTAKALALFKRIIAEHPDSDAALQAKNQLDILDII
                     EKDNASAKNEESAVKKP"
     gene            22368..24443
                     /gene="fusA"
                     /locus_tag="PKJ16_02605"
     CDS             22368..24443
                     /gene="fusA"
                     /locus_tag="PKJ16_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV45903.1"
                     /translation="MPFQKVRNIGIAAHIDAGKTTVTERMLFFTGTTRKLGEVHDGQA
                     TMDFMKQEQERGITIASAAISCKWRNHQVNIIDTPGHVDFTVEVERSLRVIDGMIALF
                     SAVDGVEPQSETVWNQADRYRVPRIAFVNKMDRIGADFAECIRQMDEYLDANPVALQV
                     PIGAEDNFTGIIDIVDRTAVTFKEFDREVGDIPAEYAEAADAARATLIEKLADFNDEI
                     ADKYLNGQEVSNELIKRAVRESTIKLNITPVLCGAAYKNKGVHILLDAVVDYLPSPID
                     KGAVTGSDIDNPEKTHLRHPAASDPFCALAFKLIHDPYVGQQTFIRIYSGELKSGMQV
                     YNSSKGKTERIGRIMKIHAKDREELDTAYPGDIVALIGMKVTKTGDTLCDEKHPVLLE
                     TIFIPPSVIELKVTVPSKKEEEKLGIALRKLSMEDPSFHIRVDEETNETIMAGMGELH
                     LEIIVDRLKHEFDVEAIVGEPSVSYREAISAEAEYNYKHAKQTGGRGQYAHCVMRIEP
                     NPGKGFEFVDHIKGGVIPAEYIPSVKKGVESVLEDGVLAGFPVVDVRVVLIDGSYHDV
                     DSSDMAFRTCASICFKEAFRKASPKLLEPLMKIEVNTPDDYIGDVIGDLNRRRGKIEA
                     MRRFRKGAQKVNGFVPLMEMFGYATQLRNLSSGRANYSMEFHQYVPLNKDMQEKTLAA
                     LKEKKLQKV"
     gene            24587..25057
                     /locus_tag="PKJ16_02610"
     CDS             24587..25057
                     /locus_tag="PKJ16_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45904.1"
                     /translation="MKSPDFHQDFLDMICALESAGCRYIIVGGYAMAFNGYVRATGDI
                     DILVEPEKDNALKVMHALSEFGAPLHGVTAEDFGKEGTIFQIGLPPVRIDIITKIDGL
                     TFDEAYADRNYHMTDDAKIPFLSLDSIVKNKNASGREKDILDIKELLKRKEKRT"
     gene            25462..26391
                     /locus_tag="PKJ16_02615"
     CDS             25462..26391
                     /locus_tag="PKJ16_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HNV45905.1"
                     /translation="MARIGIIINPHAKKNKRARINPLNRYRELGGDLADVRATQSLDE
                     VFTVAESFKKSGIEYLGICGGDGTLHHVLSRFMYVYRDAPLPPIVILKGGTMDTVSRS
                     IGLTGAGPEIMRRLMDRLADGGAIDLFSRDTIRIGDRYCFLFGLGLTSNFLTEYYRGG
                     DTGPLKAVTVALKGIASGILGLRSGNLFTRFTGRVIVNGEELWFSDFLGILAGTVEEI
                     GIGFRPLSRAYERDGAFHTIFAGCKPITVALQVNRFRTGRPLRDPNYHDCVTDRFIVT
                     GPPKFQYMMDGDLYEANSTLTVESGPAVRLVRV"
     gene            26404..27366
                     /locus_tag="PKJ16_02620"
     CDS             26404..27366
                     /locus_tag="PKJ16_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45906.1"
                     /translation="MFGIVKKKQFGIIINPVAANNRKDPAAVVRRYEKIAGMAKPPMW
                     ITSRLDDIYPIAEEFKAGGMKYLLLSGGNGTFHHVISRFKTVYQGGELPPVLMLKSGH
                     MDTIADTINLAGNDIDILERFMRALRRGRGITLLRRNLIKIEDTYCTMFGAGGLVAQF
                     MNHYYKMGEYTPQNALEVLTRFMTLARDASSREAREIFRPFKSTIAIDGRAFPKKEIL
                     ACVGVTIPGIGLGFTPGSRVLEQSGRFQFICTALSPIDFATQIFRFYGGKKIEGPNHF
                     DDLAESVVISSKDPFEYTMDGEMYHAIYELRIAASRTGVNLVHV"
     gene            27388..28623
                     /locus_tag="PKJ16_02625"
     CDS             27388..28623
                     /locus_tag="PKJ16_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV45907.1"
                     /translation="MMRFRDRYNRNVILTGLTSFFTDISSEMLYPLIQAFVAMVLTAQ
                     KALLGPVLGIIEGVAESSASLIKVFAGYHSDRTSSRKAPTIAGYSLSALSKVALFAAS
                     LGWYFVLLSRFVDRIGKGIRSAPRDALIAESTPKELQGRAFGFHRAMDFAGAFVGALV
                     CYLIVERFLDPATGTLRDLDAFYLLFAVSIVPAVLGVGFLLFTRETGGDAIGTETERP
                     RPNLNIAKYDPRLKTFFIAQLLFTIGNSSNQFLLLRSMDLGHALSTVILMYIAFNLVT
                     AALSSAFGSLSDRIGRKSLLIGGYLLYAVVYLAFGFIATGTNWLLWAFWPAYGLYYAM
                     TEGVEKAFVSEIAPAGSRATALGFFHTIVGIGLLPASIIAGLLFAFNPAAPFVFGGLM
                     ALIAVLVLGVGVSARPAKA"
     gene            28702..28989
                     /locus_tag="PKJ16_02630"
     CDS             28702..28989
                     /locus_tag="PKJ16_02630"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV45908.1"
                     /translation="MQERRSHQRRSAAVGIEISIDGRALGGEIINRSESGMLVRIDPD
                     DVTISAADIGTEVLFELDADEAHDGRRSRARLVRVADAAGSTLVALFILRD"
BASE COUNT         5520 a         9367 c         8884 g         5356 t
ORIGIN      
        1 ctgctggact acgtgcgcat ggagtgctac aacctgcagg tgtccttcca ggacttccgc
       61 gacggcaagg gcggctgcaa gtaggcgatg tgcggccggt ttagcctcac gggcggcctc
      121 gaggcgatca ggcggctcat cgaggacctc gagagcgcga ccgagctcgc gccgcggtac
      181 aacattgcgc caacgcaggg gatcgccatc ctcatcaatg acggaacgcg cctggtgcgc
      241 cttgcgcgct ggggactcgt cccggcgtgg gcgcgtgacg agggcatggg cgagcgcctg
      301 tttaacgcgc gcgcggaaac gctcgccgag aagccgtcgt tcaaagcgca attccgggcg
      361 aaacgttgcc tcgtctttgc cgacgggttc tacgagtggc ggaagacgcc gggcagcgcg
      421 aaaaaaacgc cgtattatat ccggctgata gcgcgggagt cgttcgcctt cgccgggctg
      481 tgggacctct ggaccgaccg caccgacgcg tcgcgccgcc tgttgagcgc gaccatcatc
      541 accacatcgc caaacgagct ggtcgccccc atccacgacc gcatgccggt catccttcca
      601 cgggaaaccg tatggcaatg gctcgcaccc ggcgatacgc agctcgcggc gttgcagaat
      661 ctgctggccc cctatccgcc ggagaagatg gaggcctttc ccgtatcgct ggcggtgaac
      721 aatccgggga acgaatcccc ggactgcgtt gcgccgctat cgagctaaga aattattcca
      781 gcagggattc aaatgagacg gcaggatgtc acagcgagca ggcagcccgg attggctgcc
      841 ggagccgcga atacacggat gtattgttcg cggcggggcg agcgtgacat tctgccgtct
      901 catttggtac tattgataat gaacctaatg gaataggctc tagacgaccc ctttacgcac
      961 acaggccgtc ggtttcccgg acggcctgcg cattcgctgt ggatgatgtc tcggcgccct
     1021 agaaatcgta caccttgacg atcttcacgc tccagacatt ggagcgggat gcttccttgc
     1081 agtctttctt catggtcttt ttctcgccgg cgccgagctc catgtagatg gtgcactcgc
     1141 ccgatccgca gggcggctgt ttgcgcgtct ggtcttcgag acagatgcgc ccgttcaggg
     1201 tcttcttcgc cccgctcgtg ttctcggcga ccacctcgaa ggtcttgaag cccttgatct
     1261 cgtcggccat cttcttcacg gtgacggtca agccgttctc ggtcgccgtt ttttcggcat
     1321 cgccttgggc gccggcgata tacggcgcca tcatgagcgc gaatgatgcg atgatagcgc
     1381 agagtgcgaa tcgtttcatg ggttcctccc gtagtatttt attttttctt catttcaagc
     1441 gggcacaggc gcacatacac gatgccgtcc ttgatgccgc gctcaatcac gcgatgttcg
     1501 gtctcgcccc aaaaacggca gaggacctgg tcggccacat ggtccatgtg atgcttgcat
     1561 ttgatgcaat cgtcaatttt catcggcgct ccttcgaaac ggcggtttcg cgaggagaca
     1621 taacgggacg accggatttt tttccactct tttttatttt cgtgctatga taagctgcca
     1681 cgcccggtcc gaaaggtgtt ccactgccgg ggactggaag ccggcgtcgg cgagccagcc
     1741 gaagtactca tcgcgagtgt acacgttccc gccggggctc tgcgtaatca tgtgcatcgc
     1801 caggcgcgcg ccgagataac tctcgccgcg gatattgtcg aagatgacga tgatgccgcc
     1861 cgcggcgatg cggtcgcgcg ccatgcggat catgcgggca ttgtcgtcca cgctttgccc
     1921 gtggcagata ttgcccagga aggcgatgtc gaacacgccg tcggggaact ccttcgtcgc
     1981 atcgccgtcg gcaacgctga tcccgggaac ggccgcgagc tcacgtccca ggaccgcgtt
     2041 cgattccgga agatcgtaaa tgcagacctg caggccgcgc gcggcgaact cgcgcgcaac
     2101 ggcgcccggc gccccgccca cgtcgatcac gctgcgcgcg tcgggatggc gcgcgaggca
     2161 gcggtccacg atctccggaa cggcccgttt cttccacggg gaattcatgg cgcggatgaa
     2221 atcgccaatg gaaaaattcc ggtagctttc ggtgtcgggc ctgccggtcg caagcaccgc
     2281 ggggagcgtg cgccagggat cgagaaggta atacaggaac cgccagaagt cgccttcgta
     2341 ttggtcgccc tgcgaatcga ccagccgcgc ggcgacatcc gcgggaacga catagtgttg
     2401 atcccgcttc gcgaggtacc ccatctcggc cagcccctcc aggagcgcgc tcgcgcaccg
     2461 gagatcgaac cccatggccc gggcgagctc gccggcgctc atcggctccc ggtgcaggcg
     2521 ttcgatgacg cccagcgtgt gcgcctgcgc cacgaagatg aactcctcga gcgcctcctg
     2581 cagtgcgcgc aattccatgt ccaaaccctc ctccccttcg cgcgtaggac gcacgaaggc
     2641 ccatgcgcgt caccacgacg ggccgcgggg ccgcgcgcgg atcagcgggt aaaatcgacg
     2701 accttcggat tcaccagacg ccggaagtcc ttgtaggcca tgcgcacaag ctcgctgtgg
     2761 gtcccggcgt tgaacgcaat ctcctcgtcc cgctccagct cggcgcacga gtacaccgcc
     2821 atcccgtaga gattaccgaa gggcggcatg gcccccggct cgcagtcggg aaagaggttg
     2881 gcgaactcgc tctcggtggc gagcgcggcc tccttcgcgc cgagcttgtc cttcagtatc
     2941 tccaggacga gctttcggtt ggcgggcatc accgccatcg caaacgcgcc atcgaccttc
     3001 acgatcacgg tcttggcgat ctccttgccg gagatgtggg cggaatgcgc gatgccctgc
     3061 gccgtgtagg ccggcgagtg cataatactg gtgtagagga tgtcgttttg gtcaagaaaa
     3121 tctttgagct tttttcccgg catggcgacc tccgtatcga ggcgaacgaa cgcctcgcga
     3181 tgattgttcg ggtgttcccg aatatcaaga taccgcaccg ggggcgcaaa ggaaagacaa
     3241 agtgggcgat tccgagccgc agagaaaaaa accgccgcac cgggcgcggg gaggaaaccc
     3301 tccgcccggc gccggccgtc aatcactcgt ataccttctc ctcgacctga tcggcgccga
     3361 gggcgtcctg ctccacgtcg cggaccggct ccttcgagag gaccttgaag tattccttgt
     3421 accattcggt ctggatgatg ctctgctgga tctcgttggt gcccacccag atggaggcga
     3481 gacggatgtc gcgcacgatg cgctcgagcg ggtacacgct ggtgtagccg atcccgccca
     3541 tcacctgcat gcagttgttg gcgatctgcc aggccccctc ggtgatgaac ttcttggcct
     3601 gcgaggccat gcgccggacg cggccggcgt gcgccgtctt gctgtcggcc gcgagcgcgg
     3661 cggagtagcc gaccgcccgg agcgcgtcga gcatcatcac cgaatcggcg accttgaacc
     3721 ccacggcctg gaagttcatg atgggctgcc cgaaggcctt gcgcatggag gtgtatcgcg
     3781 tcgcgatctc gaccgcgggg cgcaccgagc cgatggtcat gagcgcggtg cccagacgct
     3841 cggggatcat gttcttcacg aacacgtcga agccgccgtt gatgccgttg agggcgtagc
     3901 gctccggaac gcgcacgtcg ttgaagatca cgcggcccgc cccgccgccc cgcacgccca
     3961 tgaggccata gaggtatttc gtttccacgc cggcgtcgcg gggaatgaga aaacaggtga
     4021 gccgcttgtg cgcgggggcg ccgggatcgg tcaccgcgta ggtcatgaac cagtcggccc
     4081 cctcggcgcc cacgatgaag cgcttctgac cgttgatgat ccagtcaccg ccgtccttgc
     4141 gcgcgacggt cgtggcgccg aagaagtccg atccgccccg cggctccgtg aggcactcgg
     4201 cggcgtacac atcgcccctg agcagcggaa ccacgatcgc ctgcttgagc tcgtccgagc
     4261 cgaagagcgc cacggcctcg cagacgatgt cggcgcccac gccccacagg caggcgagcg
     4321 aataactggc cacgccgatc tcctcggcaa cgatgccgtc ctccacccag cccagtcccc
     4381 ggccgcccag ctccttcgag atgcggatgc ccaggagatt gcgccgcccg cactccttga
     4441 ggtactcgtg cgggaaatgg agcttctcgg cgtccatgtc gaggatcatt tcgcggggga
     4501 cccagcgggt gaactcgcgc gcctcgtcgc ggagcttctt ttgcgcgtca gtaaggatga
     4561 aatcgaacat tgtgacctcc tgctgtgtga ttgtgaacta cttcttcatc aactcccgcc
     4621 gcctcttgtt cagcttgcgc ttcaatgcct cgaggtcggc ctgaaagaga tcgagttcgc
     4681 tgcgctgccg ggcgacctcc ttcattttct cgtcgatata atagaggctg gtgtcgatct
     4741 ggcccatctc attgatcgcc tcgtccgcca tgccgatcat ctccgcgatc tgatcgaggg
     4801 tcgcgccgaa gcgcttgccc cgcaggatga gcttgagccg cccgcggtcc tgccgggtgt
     4861 acacgcgctg gttcccccgc gtccgctccg ggagcaagag gcccttctgc tcgtaaaagc
     4921 ggatcgtcga gggattgatg tccagctcct cggcgagctc ggatatggtg aatcgttctt
     4981 ccggcatggt ccttgcactt aaagttaaag ttaactataa gttattgacc gtactttttt
     5041 tttcaaggaa ttttcagggc gcggtacggg tgccattacc acaccccccc ctaacggggg
     5101 gggggggggg gatcgtaagg tcggtgctga tgaaaagttc aaaactttat gcaccttctg
     5161 caggagcacc ggtccggggg aggaccgcac atgccttcgg ggatattgca ttgtgagccg
     5221 atttctgcgt ctgcacgacg gcccgcatgt gttggcaacc gcgaaccgcg tgcaacaggc
     5281 ctacgtcatg ggcgcgcgcg ttatctgacg taggttacgt cggcgggccg cgattccgga
     5341 aagagtatct tcgggatgtc gtcgaggcgc tgccggagat aggccgcgcg cccggtcgcg
     5401 ccgtcgcgct cgagcgcctc gatgcgctcg ttcatgcgcc acacctgaat gcgacggcgc
     5461 tcgcggccgt cctcgcggta tatctcgttc tcggtccaca gcggaaggac ctcgtagcgc
     5521 gccgcgagcc cgccccgcca cgcgcgctcg aggacgagcc gcacgaggac cgtctcctgc
     5581 gtagtgtacg gcagggtgcg aaggtggtcg tgatacttga agcgctggtt ggaaatgaag
     5641 ttccccaggc tgtagaaaat gcggcaggat cgtccgtccg ccgccgtgta cgcctccacc
     5701 ggctggagca cgtgcggatg gtgcccgatg acgaggtcgg cgcccgcctc gcagctgtcg
     5761 cggtaggcgc gcatgtcctc ggcctcgggg cggatcttgt actccttgcc cgcatgcgag
     5821 acgaccacca cgaaatcgca gcgcgcgcgc atggtgcgaa tgtcggccgc ggtcttgttc
     5881 cgccagtaga gccagttcac gtgcaagccc cgcgggcgca ccgggaagcc gtaattgagg
     5941 atgcccgtat acgcaaggaa gcccacgcgg ataccgtgta tctgcaccac gatgccgcga
     6001 cgcgccgtcc cctcgtggcg gttcacgccg atgaagctcg ccgcgtagcg ctggagataa
     6061 tccatcgtgt cgatggcccc gcgattgccc tggtccagga tgtgattgtt ggccagcgtg
     6121 aagagcgtaa atcccgccgc gtgcatcgcg ggaatgatct cgggcgcgca attgaagatg
     6181 acgcccttgc cgtgccgaaa cggcggcgac acgggaaact ccatgttgcc cagcacgaca
     6241 tcggaatcgg ccagatgggg acggatccgg tcgaagagga aatcgaagcc gccgttgttc
     6301 gccgatccgg ccccgccctc gccgggccgg ttgttctgcg cggcgctctc cttcaccgcc
     6361 gtgtgcatca tgatgtcgcc ggtgaaggtt acgaccatgc gctgcgcggc atgcgcgctc
     6421 cccggcgcgc cgatgaggga gatcagggcc gcgaggacgg ccgccagcat ggtatatcgg
     6481 ttcacgcgat cactcctgaa atgtttttac gaggtacacg cggtttcccg cctggttgaa
     6541 ccggacgcgg gtaaagatgt ttttggccat catgatgccg cgcccgtgca ccatgaggtt
     6601 ctcgttcacc tccctcatcc gcgccatgaa tccggcgtgg tcgaaccccg ggccctggtc
     6661 cgtgatgcga taggcgacgc ggcggccatt gagcgaatat tcgacgacca ccgtgcggtc
     6721 gcggtagcgg gggtccgcgc ggcgctgcgc gatgaactgc aggtagcaat cgttggcgat
     6781 ggcctcggtt ttctcattga aggtgatgca caggttcccg tgctcgatcg cgttgaggat
     6841 catctcgcgc aatgcgatgc ggagatactt cacgttccgg gcgtcgagaa accggacgca
     6901 gttgcgcgtg agccgcatgc tcagctcgtc ggcgctggtg atgtagttgc cgatctcata
     6961 gcgctgccgc tcggcgacga agtgccgcag caggaagtcc tcctctatgc cggtcgcctt
     7021 gcccaggatc tggtttttcc cgccgatggt gatcatctcg aagcggagca tcatctcctt
     7081 gggctcctgc ccgatggccg agttgagcgc ggcggcgaag cggaccggct cattggcatg
     7141 gaacagctcc gcaagctttt cctgggcgat ctgccgcgag agcgcggtgt cctcggtgcc
     7201 cgcgtagatg agcgatatga agggcgcgcc gatgagctgc gacggctcga tgcgcagatg
     7261 atcgtatacg gcccggttga tcgtttccac ttgaagcgcc gtatcgagcg tgaagatcac
     7321 gtcgcgcgtg tcctcgacga ggtgcttgta cttctcctcg gacaggctga tggcgcgcga
     7381 cgcctcgccg agctcgtcga gcttctgccc gaggtcccgg ttgagctcct cgacgcgctc
     7441 gtgcacctgg cggaaccggt tggccagcac gatcgtgatc ccgatcacga acacgaaaaa
     7501 cccgtaccgg acgaggatga cgttccggtt gaagaagacg gcgtcgacga tgtcgaaccc
     7561 ggtgaaggcg atcatgatca tcccgccgag aaagaggttg cccggcaccg tgcgcacgag
     7621 cgagcgcagg aacgcggcgc ccgtcccccc gcgcgcgccg tcccggtcgc gttgctcgcg
     7681 caccatgtcc acgagcgagc ggaccatccg gtacaggatg acggcaagcg ggaccggcgc
     7741 ggtgaactgc cacacgcgaa gcacgtcgac ggcgaaggcc agcgagaacg ggaccacgcc
     7801 gcacacgagc acgccgctcg cgattgcgtt cccgacggta aaacggcaca cgcgcccccg
     7861 gagcaccaga tcaagaaatg cgcccaccag gggaaacagg gtaaacagcg atacgaactc
     7921 gatccggcgc aggatgttcg tatcggggat gagctcctgg gcgctgaagg tccgcgagta
     7981 caggtagacg aacagcatgc aggtaaaaag cccgaaaaac aggttgaagc gctcctcgcg
     8041 ccgcatcagg aacaggaaga gatgatagat ccccacgaac aggtacaggg tgatgaggct
     8101 caggtacacg gtctcggcgt gccgtttcaa tatcgcctga tagtcgtcga cgaccatctg
     8161 gctgcgccga aacaccccgg tatcgataaa ggtcgggtcg cccacgatgc ggacggcgag
     8221 ctcgttgcgg ccctcgcgca gggcgccggg atgcagggga aagcgcacaa agcgaagatc
     8281 gcgatgctgc gcgatcgcgc cgtccgggcc gcgatgcatc tccgaccgga cgagcgcgcc
     8341 gttcacgaac acctcccagt tttcggcgat ctgcgagatg tacagtccca ggatgcgccc
     8401 gccgcccgtt gcgcccgccg gaagctcgaa ggaggaccgc agggtgaatt cgaccggagg
     8461 aaatttccac ggcgtgaagg cggtgcggga agggtagcgc gcaacgcctc ccccggtaag
     8521 gcgcaccgcc gtgcagtgcc gcaagtccgc ggggaccagc cgccacgcgg gatcatccgc
     8581 cggggccgcc gtggtccagg acggcgagaa gccgtcgcgg tagtgcacat cgcccgccat
     8641 gggccgcacc ggcagtgcga cgagcgcgag cggaacgagc cacgcgacga ttcccgcgat
     8701 cgcggcgacg atttgtttag gcgcgcgtcg catcaccggt catttttcga cgtattcccc
     8761 ggcgtacgtg tttacgcaca tgtgccgata ggttcgatgt ggccccacgc cgacgccgac
     8821 tcgcaggaac ttgggactga agatgttttt gcggtggccc cgcgacggaa cgccgtcatc
     8881 gaccaggagc tggatcacaa tctcgcgcgc cgtgttcttc ccgtagctga tgttttcccc
     8941 gcaggagcgg cgccatgttc cgtagcgatt gagacggctc ccgacactgc tgccgtcggt
     9001 tccctgatgc ccggtgagac ccttgggccc ggtatcattc acctggtcct tcgcggcgcg
     9061 cgacatcccg ggcgatggta caaatggcgg aagcggcttg gcgcttttca ggaaatcaat
     9121 ggtctcctgc accgccgccg ccccctcctt ggtgaggagc gtgatcgcgc cgggacgctc
     9181 gtagcgccgc ccgcggaagt atttcagcat accctcgaga tgcgccgcgt agccggcggg
     9241 atcggtgcgg acgcggttga gctcgtcgac cacctcgcgc tcgagctgcg tgagatacgg
     9301 cgcgtccgcc gaaccgggcg tcggcgcgtc cgggcgcgtg tcgggccgct gcggaccggc
     9361 cgcatcctcg cccgacggcc ctccggcgag cgcgagagag cgcggcacgt cgacgggagc
     9421 gagccccgcg agaaacgcca ggttctccag ccgaccgttg tgaagcacat agacataggc
     9481 aagatcgacc tcgcgcccgc cgatacgcaa cgtgcagttc tcgtacggcg cggcgttttc
     9541 gggatcgacg cggggagcgg aaacgacgac gcgtttcgcc gcggtatcgc cgcagggctt
     9601 gcaaaaggca tagacctcct tctgccgggc gacgatgctg tgcgccttgc gcatcgcgtc
     9661 gcagcgcgcc acggcgaact ggtcggcgag cgtcacatct gcgagcgcga gaatggaaac
     9721 gaccgcggcg agcgccgcgc gcatcgatat gcggatcatg cggatacccc ctgttcaatt
     9781 tttaacatat tgtgtgctcg atatcatcgc acacggggcg atctgcatca caaaaccacg
     9841 agcgcccgaa cagccggcac aagagcgcga ctcccccgta aagacgcccg atcgcttcgt
     9901 cgctatcgcc ctgcacaaca atacaatcaa ccgcccacga cctcgctaca cgcgccgggc
     9961 gggcccgtat gctacaggcc taagtgtcgg tgtcaatgat aataagggaa atgccgtccg
    10021 gccggccagt tgaaaaaaaa ctgtgcttga cgccgcacgg acggaacgta gaaccatcca
    10081 acgcaggagg gtcacccatg gcggcaaaac tcatcatcat gacggcggtg tcgatcctca
    10141 tcgctatcgc ggccgtcatc gcctatcgtc gctacgacat tccgctcgcg gagcgctatc
    10201 gacgcattgg aaagaagaaa tcgtggaagg cggtgaccta tctcggctac tccgcggtgt
    10261 acatcgcgcc ggcggccatc gcctggctcg catgcaccta ctaccgtccc gatccggcgc
    10321 tcgccgcacg cgcgctcttc gtcggcgtca cggtgtcggc gtcgggcatc atcaacgcgc
    10381 tcgtgaagtc ggtcttcggg cgctaccgcc cctacgtgtg gttcgacgac aagctcttcg
    10441 ggttcaaatt tttccagcac aagtacggct tcacctcgtt cccctcgggg cactcgaaca
    10501 ccagcttctg cgccgcgctg gttttttcat atatgatccc gtacggcgcg ccggcgtttt
    10561 tcgtccttgc cggattcatc gcgttcagcc gggtggccat caacaaacat tatctgagcg
    10621 atgtgctggc gggatcgggg ctgggactcg tttcggcgtt cgccgcctac gagctctatt
    10681 ttcgcgacct gctcgcgtga gccttggcgc cgcggggcgc ccattccgag acgaaccgct
    10741 cgccgtcgcg gcgccccaga tcatacgcct cgcgcaaccc gtgcggatcg gtgtaatccc
    10801 actttgatat gcggatcggc tccgacggct ggacgtagac gaggcccgga aggcgcggca
    10861 ggcgctccgc cggatagacc cgcgtgagga gcaccagcgc ggtcccgtcc accggatcga
    10921 gcgcccggac cgggacattg tccaccagcc cgccgtcgag caccgggcgg ccgtcgcgcc
    10981 gcattacggg cacgaagggc ggcgtgcacg acgactgcag aatgagctcg gcgagatcac
    11041 tcggcgtcgc gcaggaccgc accggcaccg cctcgccgcg aaagccgatg cgccgcgccc
    11101 acgacgagtg gaccggaaac gacacctttt tctcgagcga ataggcgcct aaccccacga
    11161 aggtgcccga aaccggaccc agccagcgcg gcggtcgcgc aatgagaatg ttgatggcgt
    11221 gccggtggag gcgcgtgagc gccgcttcgt cgaggcagtg cgtgagcgcg tcgcggtaca
    11281 tcccgtaatg cggaaggcac ggttcgctgc cgacgatatt acgcggatag aagttcttcg
    11341 gattgcgcgc ggtggcctcc gtgaagtact caagcgccgt ttcggttttt cccgaaaaga
    11401 tcatgcaggc catcgccgcg cccgcggaca cggcgctcac ggcctttggc cgcagtccga
    11461 gcgccggcgc ggcggtctcc cagaagccaa cctgccagat gcagcggttc ccgccgcccg
    11521 cgaagacgac gccgtcgatg cgcgttgccg gttccctgcg ccgcatgcgg cttacatgct
    11581 cccgccggcg ttctccgtcg cgccgcgccg ctcgatataa atcataacgg cgacgatgac
    11641 gcaaccggcg gcgaaggccg ccagcgtgat aagttcattt gcgccgacgg acgcggggag
    11701 ccggttggcc agatagaccg tctcggcgcc gacctgcatc gattccttga agggccatat
    11761 cacccagagc gaaccgacga ccaggcccag gagaaacccc agggtgggat cgtagaagcg
    11821 cccgaggaga tagttcagga tgcgcgtgaa caccacaatc cccccgaggc agccggcggc
    11881 gaacacgccg aggatgagca ggtcgagcga cgatatggcc cgcaggatat cgaaataacc
    11941 gcccatgaga aggagcacga atgaaccgct gatccccggc aggatcatcg ccgagatgga
    12001 gatcgcgccc gccgcgaaga agaacccgag acgcgcaaca ccgacgcccg cgcccgccgg
    12061 ggcgcccgac tccgtcgcct gcagggcgat ccgctggcgc gcctcggcct tctggatgat
    12121 ctcctcgcgc gacatcgact cggagacggc gatgatgccg gcgacggcga tcaggaaggc
    12181 gagaatggtc ggtaaggttt tcttcttgat gagcgaccag ggcgtgaagg ccgacaccag
    12241 caccaggccg aagaaaaagc cgtaggtggg atcgtgctgt ttcaccagca gatacgacat
    12301 aatcttggcc agcgacccga tggatatccc cgcgccgatg gcgagcttga ggaggaacgg
    12361 cccctcgatc cggtcgaact cggccttaaa cgcgtcgatg cgctcccgcc ggagcgtgag
    12421 cgccttgaaa aacgacagga cggtcgcacc ggaaatatta tgaatgccgc cgataagccg
    12481 ctcgtaaaaa ccaagcacca gggccatggt gcccccggat acgccgggaa tgatgttggc
    12541 cacgccgaca atcaccccgt agaaaatgtc gcgaacatgc tgcttcatgt gccctcctac
    12601 tatcgtgtcg ctgcaatcgt tccgccgcgg cggctacagt acgggaccgg tttttttcgt
    12661 caaccggttc ctcgaatgcg cgttcaatcc tgaatggacc cgtcgcgcaa taggcgccat
    12721 gttaacgtac aatgaagaat tcggcgaaaa tcttatgttg acaaaaaaca tattcggcct
    12781 tttgctatat cgaatatatc aacacgccaa tccatacgga agcaaaccga atgaacgttg
    12841 taatccttgg cgcggggctg gtgggctttc agctcgcgga cgaactcatc cgggaaggga
    12901 acgatgtcgt catcatcgag aagaaccccg accgcgcgcg ctacgtggcc gggcacctcg
    12961 actgcatggt catcaacgac gagggcacgc gcctgtccac cttcaagaag gcgcggctcg
    13021 cgcgcgcgga cttcttcatc agcgtcacca acgtggacga ggtgaacatg atctcctgcg
    13081 gcatcgtgtc cggcgagtat cccgacacgg tccgcatcgc gcgcgtgcgc aacgccgact
    13141 actcgcgggc gggcatcctc agcaagagct ttttgggcat cgacttcgtc gtgaaccccg
    13201 aggtcgagac cgcgcggaac atcgccaatg ccgtggccct cggcgccgac agcgacgtga
    13261 tgctcttcga gaactccgac atgcagatcc gcaacctcgt ggtcaagccc acctcgtttt
    13321 tccgcggccg caccatgaag gacatcaaga agacgctcga cgagcccttc ctcgtcgccg
    13381 gcatcgcgcg gggggatgac ttcatcatcc cctcggggga caccgccgtg ctcgagcacg
    13441 acaacatcta cctgctcgcc tcgaagaaca cgctcaccag gatcttcatc aaggccggaa
    13501 aaaagaagga gaagatcgac cggctggtga tcgtcggcgg cgggaagatc ggccagctcg
    13561 cttgccagca cctgatccgg accggccgcc gcatcacctt cgtcgaaagc gactacgaga
    13621 tctgcaaggc gctgtcgagc cgcttcaagc gcgcgttggt gctccactcc gacatctccg
    13681 acgaggacat cttcgagcag gagaagctca gcgaatacga cctgatcgtg accaccacga
    13741 acaacgagga gctcaacatc ctcacctccg tgtacgcgaa gagcctgggg atcaaaaagg
    13801 cgatggcgct ggtgagcaag gcgagctaca tgcccatcgc cgcgcgtctc aacatcgatt
    13861 ccatcatcaa ccccaaacac agcacggtgg acgccatcct caagttcctc cggcgcggcg
    13921 acatccgcag cgtgcacagc ctctttggcg gcaaggccga ggtgatcgag ttcatcgcga
    13981 gcgagacctg cacgctcgcc ggcaagcgcc tgatggacat cgccatgccg cacgactccc
    14041 tcatcctcac gatcctgcgc gagggcgagc gggacatcat ccccggcggc gattccgtca
    14101 tcaggaccaa cgacaggatt gtcgtgatat cgtcgaagga atcggtgccc gaggtcgacc
    14161 gcttcttctc gggaacggac cgctaaccca tgaatgtcct gctggtcgtt aaaatcctcg
    14221 catttctgat gctgctgtac tcgctgttca tgctcctccc gacgggcgtt gcggcgtact
    14281 accgcgagta tgccgcactc aagaccttcg tcggcaccgt gctggccgtc gtgagcggtg
    14341 cgggactttt atatgtcctg ctgcgacgcg tgaagtcggc ctcgctcacg atgcgcgacg
    14401 gttttttcct cgtcaccgtc acgtggctgc tcgcatcggt ggtgggcgcg gtcccgtttg
    14461 tggtctcagg ggcgattccc tcccccgccg acgcgttttt cgaaaccata tcgggtctct
    14521 cgaccaccgg cgcctccata ctcaccgacg tcgaggcgct cccgaaatcg atcctcttct
    14581 ggcgctcgct caaccactgg atcggcggca tggggatcgt cgtgctcgcc gtggcgattc
    14641 tccccctgct ggggatcggc ggcatgcagc tgatccaggc cgaggcgccg ggccccacgg
    14701 tggacaagat cacgccgcgc atcgccgaga ccgcaaaacg cctctggctc atctacgcgg
    14761 gcttcagcgt cgtccaaacc gccctcctca tggccggggg catgggctgg ttcgattcgc
    14821 tcatccacac cttcggcacc ctcgccaccg gcgggttttc gtcgaagaac ctgagcgccg
    14881 ggcactataa ctcggcctat ttcgacggcg tcatcacgct gttcatggtg ctcgcgggaa
    14941 tcaacttcac cctgcactac cgactgctca ccggcaggat gggttcgttg tggcggaaca
    15001 ccgagctcaa ggcctacctc gccatcttcg ccgcctcgac gctgttcatc gcgttcggtc
    15061 tcctcgggaa cacctacgcg accttcggcg aatcgttccg atacgcgagc ttccaggcgg
    15121 cgtccatcct cacgaccacc gggttcgcaa cggccgacta cgaaaagtgg ccgctcgcct
    15181 gccaagtgac actcttttta ctgatgttcg tgggcgggtc gttcggctcc accggcggcg
    15241 ggatcaaggt cttccggatc gtcgcgctgc tcaagcaggc gatcaacgag atgcgctacc
    15301 tgctgcaccc gcgcggcgtg tttacgctgc gcatgaacgg cgagccggtg gggaaggcgc
    15361 tcgtcttttc catatcgggc ttcttcttcc tgtatgtgat catgcttctc atcacctcgg
    15421 ccgtcgtggc cagctccggg caggacctgc tcacatcgtt caccgccgcg ctcgcgaccc
    15481 tggggaacat cggacccggc ttcggcgcgg tcggccccgc ggacaacttc gcctcccttc
    15541 cggattacgt gaagtggttc ctgagcttcg ccatgctcgt gggacgtctc gagctctaca
    15601 cggtcctggt gttgtttttg ccggcgttct ggcgcaattg acgcgtcgat caaatccttt
    15661 tgttacggcg cattttcttg cgggctaccg cctgcatgtc cacggcgggg tcgaattcgt
    15721 ccacgatctc aacgccgaga agcgattcaa tcgcgtcctc gatggtgacc acgccgatat
    15781 aatcgccgtg ttcgtttacg acgaatccca tgtggacgcc cgttttcgta aaccagctca
    15841 gcagcgcaag caccgacatt ccgccgggaa catattcggg ctttcggctc aattcacgaa
    15901 gggaaacctt gatctttttt tcggccaggc aattcatgat gtcccgcctg agcacgacgc
    15961 cgtggaccgt gttcgaccgg ctgtcataga gcggcaggcg ggaaaagctc aggtcggtcg
    16021 ccgtatccgc gacgtgggtg accctggcgt cgggatcaag cgacacgacg accgttcgcg
    16081 gtgtcattat atcctggacc ctgaccttct tcaacttgag tacgttctcg attatcacga
    16141 actcgcctga atcgatggcc ccttgccgaa atccgatctt cgcgaaattg aacacttcat
    16201 ccttgctcac ttgatcgctg tgatttcccc gggcgataag ccgcgacaga aggttcatcg
    16261 gaacgatgat cggcttgagc agcgtgacca tgatcctcaa caggtatgcg ctcaccgggc
    16321 tcagccgctt ccagtacctg gccccgatgg tctttggaat tatctcggac agcaccagga
    16381 taatcacggt gaggacggcc gaaaaggcgc ccatccattt cgagccgaaa atctcgacgg
    16441 cgatcgcgcc cgatacggcg gcgcccatcg tgtgcgcgat ggtgttcaat gtgagaatcg
    16501 cggcgatcgg ctcgtcaata tcgtccttca tcctttgcag gatggcgccc gatttctttt
    16561 tcgcgtcgat gagcgtctgg atgtacggtc gcccgacgga gaggatgacc gcctccaaga
    16621 gcgagcacag aaaggacaga gcggtaaaaa gggtgataat gagcgttaat tcgaacatgc
    16681 tcgagttgga cctcccattc gcgtcgtagc ggacgcgacg ccacgttggt cgcagcaaga
    16741 ttcacgcatt cgccaggata tggaccgatc cgaccaccgt caagtacatc ggcgtctccc
    16801 ggtcgcgcgt cgcgacacgg gcctacgctc tctgcacgcc ttcggacggc tccgccgttc
    16861 agatttgatc ctacaatacg accgtcgcga ggcccagata aaagaggatc gtgctgatgt
    16921 ccgtgatcgc gagagtgagc ggcccggcgg cgatcttcgg gtccaagcgc aggcggtgca
    16981 ggagcgtggg gatcgagagg ccgtagaggc atcccgcggc gatcaccaga aggatgctcc
    17041 cgccgacgga gagggccgcc agccccttcc cgtgccacag ccagatgaac gcgccgacga
    17101 tggtcccgct ccctgcgccc agcagaaacg cggagcgcag ttcccgccag aacgcgctca
    17161 tgtaccagtc caggttcggc cgcatcgccc ggagcgcctg gatggtcatc gtcatcgact
    17221 gcatgctcac gctctcgccg agccccagca ccagcgtcag gaaaaacgcg aggatgatgc
    17281 tcgtcgacaa cgtcgcctcg aagacgcccg ccagcatcgc gcacagtaag ccgctgccga
    17341 tggtggccgt gagccacgga aaacgatacc ggaaagcgcg cacgggagac gcgtcgcaca
    17401 cctgcatcac ccggaagccg atgagctcga agagctcctc gacgcggtcg cgctcgttga
    17461 tatcgaacga gacgccggtg agcatgctca cgtccaccag gccgatcacg cgggcgtcgt
    17521 cgtcgacgat gggcagcgcc aggagcttgt tctccgccag ccgctgatgc gcctcgatga
    17581 tcgtcgcgtc gctccggatc gtgatcacat cggcgatcat gatgtccgag agccgctggt
    17641 cgagcgggga aacgagcaac cgccgcgtgg gcagcacccc gacgagccgc tcgtctgcgt
    17701 cgacgacgta aaaatagagg atgcgctcgc caagtccgcg cgaccgtatg tcgtccaggg
    17761 cccggccgat ggtgagatcg tcgcgaagca gcgcgtaatc ggtcctcgcg atggacgcga
    17821 ccggctcgtg aatatcgact atatggtccg tgctcgtgtt cctattcgcc atgactcctc
    17881 catcgggatt gcttcggaac ggacaggggc cgctgccgta tcgtcgtcga ccggaaacgg
    17941 cttttcagca ggcaaacgag cgcacgccgg ggacgatcag cacatcacag ggcaccgatg
    18001 agatgatgcg cgtcaccctg ccgccgaaca ccgcatcgag gagcggcatg cgccgtgtcg
    18061 cgccgaccac cacgacgtcc accgaggaca ggcgggcaag ctcgcgcaat agcggcagct
    18121 cgtcgccgaa ctccaccaac tgcgccatcc ggtctcgctg acgcgggctg aggtccgcgc
    18181 cggcgatgaa ctcgcgcagg tcgcgctcgg caacggcgcg catctgctcg atgtagcggt
    18241 cgcgttcgcc cacggccgtt gcgcgcgccg cggcgaaggc gtacagcgcc gaaatcgatt
    18301 ggtcgggaaa aaacgcggcg gcggtttcca cggcgcgctt cgaggccgcg cacaggtccg
    18361 taagcgccac gacattccgg tagggcgcac gaacgcgctc ggtgaccacc aggaccggcg
    18421 cctccgcggc gcggaggatg cggtcggtcg ttttgcccag cgagaagcgg ccgagcgcct
    18481 cgttgcgcgc gacgccggtg acgatgagat cgcagccctc ctccctcgcg acgcgcatga
    18541 tgacgcgatg cggcgcgccc tcctcgaggc ggatctgggc gctggttccc gcctcgccca
    18601 ggtagtcgag cagctgccgg cgcgccccgt cgatgcgcgg atcgacaagc cggttgaccg
    18661 ggaagaacgg aatgcgccgc gcgccggacg gctccgcgtt cgcctcgagc acatgcaaca
    18721 ggaccaggcg cgcctggtag tgacgcataa gcgcaatgga ccggtccagg gcgcggtcgc
    18781 tccgtgcatt aaggtcggtg gccagcagaa tttttttcgg gggcgctatc atcatcaaca
    18841 cactcgcata tattcaattt gcgccataac gctagtttgt catccttttt ttcattaata
    18901 cttataatca cgcacgacga tagatattgt atacgcgaac cgaatccgcg gaatgagcat
    18961 gtcggcctca caaacacatc gacaggcggg catgaacacg ttagcgacag gagaatccag
    19021 catgaaaacc agcatctgga acgccatcaa caatccgcgg agcacatact atattatact
    19081 gatatacctt gcgttgtccg ttcttttttc tctctgctac tggtttattg cgccccgcat
    19141 cgagggcgtt caatcgctca tgtacaacat gggcgggcaa tcgcttgttc cggtgcatgg
    19201 atatttcgac gcgtattatt acagcataac cacgcaaacg acggtcggac acggcgacat
    19261 cgtaccggcc acgcgcgggg gaaaaatcgt caccgcgctc caggtcgtcg tcggttattt
    19321 ctacctcgcc ttcacgatct cgtttttcac ctgcaaatcg ctcgttcaat cggagacgtt
    19381 caaggcgttt ttccgaaatt acgaagacga tatcgcctcc cgctgacagg agcccgaagc
    19441 gccatgagca cctctcccgc cattcgccgc atcatccagt cccttatcgt catcacggtc
    19501 gtttcggccg tcgcatgggc cgccgatccg gggggcgacg gccacagcag cgtggtgcgc
    19561 agcgtgggca tcgccatgct ggcggcgacg gtgctggcgt acttcggcaa tctcatcaaa
    19621 cagccgctgc tgttggcgta tcttgccgcg ggggtcgtga tcggcccgtc catcgggctt
    19681 ggcctgatca agaacgttca cgacatcgaa acgatatcgc acttcggcct catcctgctc
    19741 ctgttcctca tcggcctcga aatagacgtc aagaagctca gggagtcggg gaaatcgctc
    19801 atcatcggcg gcatcaccca attcccgctc acggtggcca tcggcatcgg tttcttcatg
    19861 ctgttggggt acaccctcgg cggcgggaag tacgatctga tttatcttgc ggcctgctgc
    19921 gggctcagca gcacggccat cgtcgtgaag cttctctacg ggaagttcga actggacacg
    19981 ctggccgggc ggatcacgct gggcatactc gtcttccagg acatctgggc gatcctcctt
    20041 ctgggcatac agccgaacct cgcgaacccc gatgtggcgc agatcctgct ctccttcggg
    20101 aagggcttcc tgctggtcgc ggtgagcatg ctcatcagca agttcgtcct gccccgggtc
    20161 ttcaagtccg tggccaaggt cccggagctc gtcctggtgt cgtcgctggg ctggtgcttc
    20221 ctcgtgtgcg gcggcgcggc ctacctgaat ctctcgctcg agatgggcgc gctgatcgcc
    20281 ggggtggcga tatcgacctt cccctacaac ctcgacgtga tcgcgaaggt cgtgaacatc
    20341 cgcgatttct tcgtgaccct gtttttcgtc tccctggggg tgcagatccc gaacccgctg
    20401 gcgaacacgt cgatcctggc ggtggcggcg atcgtttcga tcttcctggt cgccgcgcgc
    20461 ttcgcggcga tctttcccgc gctctacgcg ctcaacaatg gcctgcgcgt gagcctgctc
    20521 acgcccatca acctgtcgca gataagcgag ttctcgcttg tgatcgcctc cctcggcgtc
    20581 gccgccggcc atatcgcgcg ggacacgctc tcgatcatca tcttcgtgtt cgtcatcacc
    20641 tcggtctcct cgacctacat gatcaagtac agcgacgcca tccagaagac cctggcgcgg
    20701 gccctgcggg cgatcggcct cagggagatc caatccgccg cgcaggatga tgattcggcg
    20761 cccggcaaag agatcgcgct cctgggcttc catcggacgg cgagctctct cctgcacgag
    20821 atcatggacg ccaacagcgc cgcgaccggc gggcatacgc ttgcggacaa gctcgtggtg
    20881 gtcgatttca acccggagat ccacgaatcc ctgcagagct tggggatcaa ggtggtctac
    20941 ggcgacatcg ggcacctgga cacgctccac cacgcgggca tccacgacgt ccgcatcgtc
    21001 atctcgacca tccccgacac catgctcgtg ggcacggaca acctcacaat catccgcgcc
    21061 atgcgcgata tctgcccgca cgcgaaaatc atcgtcaccg cggaaagcac cgagcgcgcc
    21121 ctgaagatgt acgccgaggg cgccgactac gtcttcctgc cgcgcatcct ctctgcccag
    21181 cgcctgatga cggtgatcgg cgcattgctg ggcgaccggc cgggcgagct ggaatcggtg
    21241 gcggccaagg agatcgaggg actcacggag cgccaggaga tcatccgcta ggcgtcctcg
    21301 cggcgcccga tgcgcacggc cccccgcccc gcgcgaaata gcatgcccca tcggtgaaga
    21361 ttttttctaa agcgtgttga caaatcggcc gatcctggga gtatattatg tcgcattgta
    21421 aaccgaggtg ccccgatgat ccgttcagcc gttctccgcg ccgccgtcgt catcctgctc
    21481 gccgcgccgt gcgcactggg cgcgcaggaa aagaaggagg aacccaatct ccgaaagatc
    21541 gccgacgaca ccaagttcca gaacgcgctc accttcatca gcctcaaaaa gtatgatcgc
    21601 gcgctcgtgg agctcgccga atacctcgag gtgttcgtaa acggcagcca ccgccgtgag
    21661 gcctatgcgc gcaccgggga catccacttc gagcgtttcg actaccgcct ggctatccgc
    21721 gcctaccgga ccctttccga ggagttctcc ggaagcccgg aggggatcga ggccttgtac
    21781 cgcatcggac tgtgctaccg caagatggga cgcaccgcga aagcgctcgc gctgtttaag
    21841 cggatcatcg ccgagcaccc cgattccgac gccgcgctgc aggcgaaaaa ccagctcgac
    21901 atccttgaca tcatcgagaa ggacaacgcc tccgcaaaga atgaagagag cgccgtcaaa
    21961 aaaccctgat ttttttgatt tatcttgaca cgatccggca tggcttgaac tatgccgcaa
    22021 ccttgggccg cgtcgtcagg tcccgcacgg acaatgacac agacggcccc cggaactgat
    22081 tcgacccaca acggacacag gatacgccgg tatggaacag aatacctatc cgaaacgatg
    22141 tgtgcggcgc gggggtatcg gtgacggtcg ggaccgggcg cggtgatgtt tcgcaggcat
    22201 tccgcttccc agcgacgttc gcgcggcgcg ccaatcagca cgcgcgcata acgccctcgc
    22261 atcccagccg tcgaacccct ccccgacgac gcattcatgg tttccagtat tacagtacca
    22321 gagcacacgc agcatcagat catatataac aggacaggag cagtatcatg ccgtttcaaa
    22381 aagtaagaaa tatcgggatc gccgcgcaca tcgacgccgg caagaccacc gtgaccgagc
    22441 gcatgctctt tttcaccggc accacgcgca agctcggcga ggtgcacgac gggcaggcga
    22501 ccatggactt catgaagcag gaacaggagc ggggcatcac catcgcctcc gccgccatct
    22561 cctgcaagtg gcggaaccac caggtgaaca tcatcgacac gcccgggcac gtcgacttca
    22621 ccgtcgaggt ggagcgcagc ctccgcgtga tcgacggcat gatcgccctc ttctcggccg
    22681 tcgacggcgt ggagccgcag tcggagaccg tgtggaacca ggcggaccgc taccgcgtgc
    22741 cccgcatcgc cttcgtgaac aagatggacc gcatcggcgc cgacttcgcc gagtgcatcc
    22801 gacagatgga cgagtacctc gacgccaatc ccgtggcgct gcaggtgccc atcggcgccg
    22861 aggacaattt caccgggatc atcgacatcg tggaccgcac ggccgtcacc ttcaaggagt
    22921 tcgatcgcga ggtgggcgac atccccgccg agtacgcgga ggccgccgat gcggcgcgcg
    22981 ccaccctgat cgagaaactc gcggacttca acgacgagat cgccgacaag tacctcaacg
    23041 gccaggaggt ctcgaacgaa ctcatcaagc gcgcggtgcg cgaatcgacc atcaagctca
    23101 acatcacccc cgtcctctgc ggcgcggcgt acaagaacaa gggcgtgcac atcttgctcg
    23161 acgcggtggt cgactacctg ccctccccca tcgacaaggg ggcggtcacg ggatcggaca
    23221 tcgacaaccc cgaaaagacg cacctgcggc accccgcggc gagcgatccg ttctgcgccc
    23281 tggccttcaa gctgatccac gacccctatg tcggacaaca gacctttatc cgcatctact
    23341 cgggcgagct caagagcggc atgcaggtct acaactcatc caagggcaag accgaacgga
    23401 tcgggcgcat catgaagatc cacgcgaagg accgcgagga gctcgacacc gcgtatccgg
    23461 gcgacatcgt cgcgctcatc ggcatgaagg tcaccaagac cggcgatacg ctctgcgacg
    23521 agaagcaccc cgtgctgctc gaaacgatct tcatcccgcc gtccgtgatc gagctcaagg
    23581 tcaccgttcc ctcgaagaag gaagaggaaa agctggggat cgcgctccgg aagctctcga
    23641 tggaggaccc gtcgttccac atccgcgtgg acgaggagac caatgagacc atcatggccg
    23701 gcatgggcga gctgcacctg gagatcatcg tggaccgcct gaagcacgag ttcgacgtgg
    23761 aggcgatcgt gggcgaaccc tcggtgtcgt accgcgaggc catctccgcc gaggccgagt
    23821 acaattacaa gcacgccaag cagaccggcg gccgcggcca gtacgcgcac tgcgtgatgc
    23881 gcatcgagcc gaaccccggc aagggcttcg agttcgtgga ccacatcaag ggaggcgtga
    23941 tcccggccga gtacatcccc tcagtgaaaa agggcgtgga gtcggtgctc gaggatggcg
    24001 tactcgcggg cttcccggtc gtggacgtgc gcgtcgttct catcgacggc agctaccacg
    24061 acgtggactc aagcgacatg gccttccgca cctgcgcctc gatctgcttc aaggaggcgt
    24121 tccggaaggc ctcgcccaag ctcctggagc ccctcatgaa aatcgaggtt aacacgccgg
    24181 acgattatat cggcgacgtg atcggcgacc tcaaccgccg tcgcggcaag atcgaggcga
    24241 tgcgccgctt ccggaagggc gcgcaaaagg tgaacgggtt cgtgccgctc atggagatgt
    24301 tcggctatgc gacgcagctg cgcaacctct cgagcggccg cgcgaactac tcgatggagt
    24361 tccaccagta tgtcccgctc aacaaggaca tgcaggagaa gaccctcgcg gccctaaagg
    24421 aaaagaagct gcagaaggtg tgatccgcgc gcggacctcg ccgccgaaag ccccggcccc
    24481 acggccgggg cttttttgtt tcggacgggg atcggcctcc cggacctgtc gagtacggta
    24541 taaatattat ttgacatccc gttgcggacc gcagagcata ttgtcaatga agagtcccga
    24601 ctttcatcag gattttcttg atatgatctg cgccctggag tcggccgggt gcaggtatat
    24661 cattgttggc ggatatgcca tggcattcaa tggatacgtt cgggcaacgg gagatattga
    24721 cattcttgtc gagccggaga aggacaacgc cctcaaggtt atgcatgccc tttcggaatt
    24781 cggggcccca ttgcatggcg ttacggcgga ggactttgga aaagagggga caatctttca
    24841 gatcggcctt cctcccgtga ggattgatat tattacgaaa atcgatggtc tgacatttga
    24901 cgaggcatat gccgaccgga attatcatat gacggatgac gcaaagatcc cgttcctttc
    24961 gctcgattct atcgtaaaaa acaagaatgc atcaggacgg gaaaaggaca ttcttgacat
    25021 caaggaactg ctgaagcgga aagaaaagcg gacatgacac gggaggaacg aaaacgatat
    25081 cggctggcga attatcacgc cgagattaaa acgcgtgaat catctgactt tatagtaatc
    25141 gctcctggaa ctgactgcgt tattgattcc gcgcgaagcc ttgccgccct ggaatcaatc
    25201 cgggcggttg tctatccgga acttgcccgc ggcatccgca tggacagaag ccgggtgaca
    25261 attctgcgcg ggcagccgag acgcactccg atgagcagcg atcactgaag ggcacagtat
    25321 tgccaacgag tggaaggaac ggagcacgtt catgtacggc cttcggcagt tcggatacgt
    25381 ggaaatcgtg atagccgata ttcaccttga ctttcgcggg gcggcatgcg tgcctttgtc
    25441 atctccgcaa ggaacgggca catggcacga atcggcatca tcatcaatcc gcacgcgaag
    25501 aagaacaagc gcgcacggat caaccccctg aaccgctacc gcgagctggg gggggacctc
    25561 gctgacgtgc gcgccaccca gagcctcgac gaggtcttca cggtcgccga atcgttcaag
    25621 aagtccggca tcgagtacct gggcatttgc ggcggcgacg ggacgctgca tcacgtgctc
    25681 tcgcgcttca tgtacgtgta ccgcgacgcg cccctcccgc ccatcgtgat cctcaagggc
    25741 ggcaccatgg acactgtctc gcggtcaatc ggcctcacgg gcgccgggcc ggagatcatg
    25801 cggcgcctca tggaccggct cgcggacggc ggcgcgatcg acctgttctc gcgcgacacc
    25861 atacgaatcg gggaccgcta ctgctttctc ttcggcctcg ggctcacgtc gaactttctc
    25921 accgaatact accgcggggg cgataccggg ccgctcaagg ccgtgaccgt ggcgctcaag
    25981 ggcatcgcga gcggcatttt ggggctccga tcgggcaacc tctttacgcg cttcacgggc
    26041 cgcgtgatcg tgaacggcga ggagctctgg ttcagcgact ttctgggtat cctcgccggg
    26101 accgtcgagg agatcgggat cggctttcgc cccctctcgc gcgcgtacga acgcgacggc
    26161 gccttccaca ccattttcgc cggctgcaag cccatcaccg ttgcgctgca ggtgaaccgc
    26221 ttccgcaccg ggcggccgct ccgcgacccg aactatcacg actgcgtgac cgatcgtttt
    26281 atcgtcactg ggccgcccaa attccagtac atgatggacg gcgatctcta tgaggcgaac
    26341 agcaccctca ccgtggagtc cggcccggcg gtccggctcg tgcgcgtata ggaggagacc
    26401 cggatgtttg gcatcgtcaa aaagaaacag ttcggcatca tcatcaatcc cgtcgcggcc
    26461 aacaaccgga aggacccggc cgcggtcgtg cggcgctatg agaagatcgc cggcatggcc
    26521 aagccgccca tgtggatcac cagccggctc gacgacatct atccgatcgc cgaggagttc
    26581 aaggccggcg ggatgaaata tctcctgctc tcgggcggga acgggacctt ccatcacgtg
    26641 atctcgcgct tcaagactgt ctaccagggg ggcgagcttc cgccggtgct catgctcaaa
    26701 tcggggcaca tggacacgat cgccgacacc atcaacctcg cgggcaacga tatcgacatc
    26761 ctcgagcgtt tcatgcgcgc gctccggcgc ggccggggga ttacgctgtt gcggcgcaac
    26821 ctcatcaaga tcgaggacac ctattgcacc atgttcggcg cgggggggct ggtggcgcag
    26881 ttcatgaacc actactacaa gatgggcgag tacaccccgc aaaacgccct cgaggtgctc
    26941 acgcgcttca tgacgctcgc ccgcgacgcc tcgtcgcgcg aggcgcggga aatcttccgc
    27001 ccgttcaaat cgaccatcgc catcgacggg cgcgcgttcc ccaaaaagga gatcctggcc
    27061 tgcgtcggcg tcaccatccc cggcatcggc ctgggcttca cccccggatc gcgcgtgctc
    27121 gagcagagcg ggcgcttcca gttcatctgc acggcgctgt ctcccatcga cttcgccacg
    27181 cagattttcc gcttttacgg cgggaaaaaa atcgagggcc ccaaccattt cgacgacctt
    27241 gcggagagcg tggtgatctc gtcgaaggac ccgttcgaat acaccatgga cggcgagatg
    27301 taccacgcca tctacgagct caggatcgcc gcgagccgga cgggcgtaaa cctggtccac
    27361 gtataacgca cacccacagg agcgacgatg atgcgattcc gcgaccgtta caaccgcaat
    27421 gtgatactca ccgggctcac gtcctttttc accgacatct cgtcggagat gctctatccg
    27481 ctgatccagg cctttgtggc gatggtcctt acggcgcaaa aggcgctcct ggggccggtg
    27541 ctggggatca tcgagggggt cgctgaatcg agcgccagcc tcatcaaggt gttcgccggc
    27601 taccactcgg accgcacgag ctcgagaaag gcgccgacca tagccggcta ttcgctctcg
    27661 gccctctcca aggtcgcgct gttcgccgcg tcgctgggct ggtacttcgt gctcctgtcg
    27721 cgcttcgtgg accggatcgg caagggcata cgatcggcgc cgcgcgacgc gctcatcgcc
    27781 gaatcgaccc cgaaggagct gcaggggcgc gccttcggtt ttcatcgcgc gatggacttc
    27841 gcgggcgcct tcgtcggcgc gctcgtctgc tatctcatcg tcgagcgctt tctcgatccg
    27901 gcgaccggca ccctgcggga cctcgacgcg ttctacctgc tcttcgccgt gtcgatcgtg
    27961 ccggcggtcc tcggggtggg cttcctcttg ttcacccgcg aaacaggcgg agacgccatc
    28021 gggacagaga cggaacgacc gcgacccaat ctgaacattg cgaaatacga tccccggctc
    28081 aagaccttct tcatcgcgca gctccttttc acgatcggca attcctcgaa ccagtttttg
    28141 ctgcttcgca gcatggacct ggggcatgcg ctctcgacgg tcatcctgat gtacatcgcc
    28201 ttcaacctgg tgacggcggc gctctcgtcg gcctttggat cgttgtcgga ccgcatcggc
    28261 cgcaaatcgc tcctcatcgg cggctatctc ctctatgccg tcgtctatct cgccttcggg
    28321 ttcatcgcca ccggcacgaa ctggctcctg tgggccttct ggccggcata cggcctctac
    28381 tacgcgatga cggagggcgt cgagaaggcc tttgtctcgg agatcgcccc cgccggttcg
    28441 cgcgcgaccg cgctgggttt tttccacacc atcgtcggca tcgggttgtt gcccgcgagc
    28501 ataatcgccg ggctgctctt cgcattcaat ccggcggcgc ccttcgtctt tggcgggctg
    28561 atggcgctca tcgccgtcct ggtgttgggc gtcggtgtca gcgctcgtcc ggcgaaggca
    28621 tgatctccgc cggggagcgc gacgcgctgg cagtatctat cgcacagcag gataatagct
    28681 tactagttgc acgaggtacc catgcaggaa cgtcggagcc accagcgccg cagcgcggcc
    28741 gtcggtatcg agatttccat cgacgggcgc gcgctcggcg gggagatcat caaccgctcg
    28801 gagagcggga tgctcgtgcg gatcgaccct gacgacgtga cgatcagcgc ggccgacatc
    28861 ggcacggagg tcctcttcga gctcgacgcc gatgaggcgc atgacggacg ccgctcccgc
    28921 gcgcggctcg tccgcgtcgc cgacgcagcg gggagcacgc tcgtggcgct gttcattctc
    28981 agggattaac atcgcggtcc ggcggtcgcc ggcgcgcgtc actcggtggg ctggccgatc
    29041 gactcgagga actcgtccag gttcacctcg cccaccggat cgatcgttat aaactcgcac
    29101 cccaccttga tgatcttgtt ctcgagc
//
LOCUS       DAORHF010000016        28605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1000_length_28605_cov_6.339370, whole genome shotgun sequence.
ACCESSION   DAORHF010000016 DAORHF010000000
VERSION     DAORHF010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28605
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1000_length_28605_cov_6.339370"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            362..1345
                     /locus_tag="PKJ16_02635"
     CDS             362..1345
                     /locus_tag="PKJ16_02635"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV45909.1"
                     /translation="MDIQLNLSAFTDERRFVPLDPAALYDVLIVGGGPAAMTAAVYCM
                     RKGVSTGLVTERFGGQIAETSSVENYLGFTYIEGLQLVERFTDQVKQFPIAVIEGSRA
                     RALAVDGPVKRVTLENGATLQARAIVITTGKHWRRLGVPGEAELTGRGVAYCSTCDAP
                     LFAGKSVAVVGGGNSGVESAIDLARIAERVTLVQFLDALTADRVLVDALAGFANVEIL
                     FHHEVTRINGTGAVQSIAVRDNATGAERDLPVSGIFIEIGLSPNSNFARGALELNEQG
                     EIAVDCACRTSAPGVFAAGDVTTVPFKQIIIAAGEGAKAALSACEYVLTQS"
     gene            1376..2017
                     /locus_tag="PKJ16_02640"
     CDS             1376..2017
                     /locus_tag="PKJ16_02640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV45910.1"
                     /translation="MAVFNEKVRGQLKDVLKGMINPVTIAFFSQEVECMACREARAFL
                     EEIAGLSDKLTLVVHDLQKDAAEAAALGIDKVPGIALLDAKGAPTRMNFFGIPGGYEI
                     NSFLGALLEASGKREALPGDIAARIATIAADVRIQVFISLTCPYCPAAVSAAHRIALE
                     SPRVRADMVDSSIFSHLAIKYQVSGVPKTVINETHELVGAQPLGKLLDIIEKL"
     gene            complement(2033..3382)
                     /locus_tag="PKJ16_02645"
     CDS             complement(2033..3382)
                     /locus_tag="PKJ16_02645"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HNV45911.1"
                     /translation="MKTGTKRTIAGAAGAALMIVYPLAMFSCGRTQHFELSRFLGPDM
                     CGGCHAAVADQWKNSMHNLSHFDPVYTAVAKHLRKGLTDPDEIAEAESCVKCHTPVGF
                     VTGNPTKVSDDLDRVPDIAKQGIQCDYCHSATGARKMYNNDIRLDPGHGMDNAGTKRG
                     PFTDSHSEFHKSAYSKFHTESGICGTCHDVRHVVYGTKLETTYEEWLGSPYNSSDSNK
                     RIPCQGCHMYQRPGVPATGSTPRPQNPGEAAGGGPQRAHIYTHYFVGANAYVPNKFGD
                     AVKARMAEERLKNAAALTIIDAELSKGIVKVEVKNIGAGHKLPTGLTDVRQMWLEVVV
                     TDAAGRELFSSGKLGRDGTIPAGAVVYNTVFGDGKGKAVANLAKAREVLKDYRIPPLG
                     SVVETYTVPNLKAKTVTVIVALKYRSAPQELLDAVFGAGKIVLPVTTMAEAKKQIAL"
     gene            complement(3411..4388)
                     /locus_tag="PKJ16_02650"
     CDS             complement(3411..4388)
                     /locus_tag="PKJ16_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45912.1"
                     /translation="MNKHILARCAAAACAALMAAGCARDASRFDEATPAASGSYRIFK
                     QTYYRQGKFREYHFYESDSAGNTFSESIQRSDGLLHGATKRYKKEGDTVKVLVYDNQD
                     NLKSYVVQEYDPVHKNIVKLSNYDKNDRLRTYITFEYDAAGTLMKKSNFDAAGKPTGY
                     QFEFDKNGNLVKDSYYEAGALRRYSVHEYDAQNNLVKRTRFDGQGRLDSYKMFTYNDR
                     GMLAEHQNYSASSALLWNMVSSYDAAGRRVKVQYFEREGGGMGLKFTKEFTYDDKGNL
                     AKVATYDRAGNITGSGVYKYDAKGNKTRQEYFDERNELLGFSIYDYDMQ"
     gene            complement(4425..5075)
                     /locus_tag="PKJ16_02655"
     CDS             complement(4425..5075)
                     /locus_tag="PKJ16_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45913.1"
                     /translation="MDTKKKRTKLILHKIQVRTTMSVMGIVFLGVAIIIALIAVSVNY
                     NHNQLSDIIKKNSHIIHRIDNIAAIQDNVVEDLMTYSYSVADPRQKLAIQEFAMDHYK
                     NITAIKKNVSDTKNNITTVQNILRFNNILLIAIIVIVVVQGIVLYYLGIRISHKFTGP
                     IMVISDFLRDFIEGKKATLRPLRKDDELREFYYLFARAVKMVRQRERDLHKRVDGK"
     gene            complement(5218..8238)
                     /locus_tag="PKJ16_02660"
     CDS             complement(5218..8238)
                     /locus_tag="PKJ16_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV45914.1"
                     /translation="MRLLNRLSTLSADAIALGASAVIIATFSTLLYADLSARLDAGDA
                     VQIGTITYKKRVAQRKYGAQVIWEDVEQNVPVFNNDSVRTSDSSEAVIRLADGTDIQL
                     EDNSMILLSMAEGAININFEHGSIFARRGDVAAKDIQQVKIVSKDANVSIEKSDVKLT
                     KDENKDLNLTVTKGTAKIETGSEVKTIKEDQRVVVSQGAKEAKVYDIRLRLAAPEANR
                     FFVTQQANQPISFSWEPVSGATEVFFEIAPDSAVSRPVVSRRVDGTTAAAVLGAGSHY
                     WRLRAKNAGGGVEYSETRKLTIVQDRPVRLTTPMNGAQFAFVARAPIIGFRWESNALA
                     TGYTVEVAADAAFTRIVRSVPANLTEIAVDSLGEGVYFWRVRTSVEIAAGGYAGSSTV
                     HQFTVSRKKALEPPVLIAPAGDRVISAGVVKSAGLMFSWRPDPQIRSYRLSIARDQGF
                     SQGVITISNNGNFYELDRPLPEGQYYWRVQGVGAPGEEPVLSAVRRFTVSSPESIRAL
                     APATGAVITVKSAEKTVPVAFSWKPAGMAGRYQFELARDRDFAVLHTRQLVSGTGVTV
                     PLGEAGAYFWRVRLIDDSRAEVMSSDTLPLSVNRLEPEKPKAVLVVIAPAAAKGAAIS
                     IDGKLMGHDMVRVEPKPGVAVRVEIAAKEFEPYQKSVTLADGQTLELRAEMAGVKKKE
                     RAAPIADMAAPIVATPVTAAGGILIVGSNDGRVSGFRKDGSRAWNTNLQKRFESAPAV
                     QQSQVFVVTVDGTLYCINAQSGAVVWSRKAAGTMLFGSKPLVVGDRVYLATGQGVVQA
                     FSTSGAELWQKRLDGGIYCSPAHHDGVVYVGTNNYRLHALKASNGAELWAYELDGRIV
                     AMPVVADGALYVGSFAGSVYSLTAKKGALNWKFLAGDAILAPPTPYRGTVYAGTSAGT
                     VFAIAADNGKERWRYRSGGKIQAEAVALRDTVYVPAGRTIFALAAATGALEWKRDFDH
                     AVTTSAAAVGDELFFGLDNGRVVSVRTDLGTRVR"
     gene            complement(8222..10105)
                     /locus_tag="PKJ16_02665"
     CDS             complement(8222..10105)
                     /locus_tag="PKJ16_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV45915.1"
                     /translation="MTIEDIAKPVRQFIYTMKIRTKLISIISLIIVTALSGMIFLASY
                     FFRSDNKLRIEESNHKISEVTALKVRADFLAVVEKLNIMGTTMLQDFPQKKLFTDLFF
                     TNDKDFIFAGIAAGSAGSPAIAVTRSIANAAFLAENQMTPAAIDAITRTAAPQFARSF
                     GGEAVVQNLSDSFKMPVIGISIPFQRDGTRVATIAIAYLKLDRFLQAFRQAGITETFM
                     VNDQGDIIAHSDSKIVLSRGNYLALPIVQRMMKSKYDNEQTRYRDTGGVYHLGSFKKI
                     GFSGIGIVATVPEEKAFQAVYSIQRRNIYIMIIFLNLAILVVFFFSKTLTTPLVRLVG
                     ATAQIEQGNFRVDIAPTSGDEIGQLTRSFVSMGQGLEERERLKDTFGKFVNKEIAEQV
                     LKGEIKLGGERKTACIFFSDIRSFTAISEKLEPEEVVEFLNEYMTRMVQCVNNTNGNV
                     DKFIGDAIMAVWGAPISHGNDTANAVSGALMMRNELMGFNRGRGGDKKPIIKIGCGIN
                     TGPVLAGQIGSDERMEYTVIGDTVNLASRIEALNKPFGTDILISEDSYAIVKDLFVCE
                     PMQKIKVKGKSEPQQIYAVLGRMNDPSAPKSLDELRAMLGIEMKGKPDAAPEEEVKYE
                     IIE"
     gene            complement(10226..10855)
                     /locus_tag="PKJ16_02670"
     CDS             complement(10226..10855)
                     /locus_tag="PKJ16_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45916.1"
                     /translation="MTEASITALGNLRTPNSFEWYLVPFLALVIYAYSLEVRSGHWDR
                     VITGIGFFGGELIWEMFNALVLHFTQYAALWTVSGKSVFLIFVGLNVEIALMFALAPL
                     ALFNALPADRGRKILGLSNRIVIPVCFGLFCVMVEILLNRWGALAWAYRWWSWPNVWL
                     IIIAYSGPFTFIAWFNDRVSLKNKAFAAAACWALAGIGFVVFAVFLRWI"
     gene            11237..12901
                     /locus_tag="PKJ16_02675"
     CDS             11237..12901
                     /locus_tag="PKJ16_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007043903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HNV45917.1"
                     /translation="MLHIDLMAPKSLEWSLQINCIYALRTIISLRSENLTGYSIMEVL
                     WRLAREEYDSVPDDLSDAFLDDLYHIFIGTTGNSRVYDREIYSVSADRHGREAAIERS
                     EQLDAMAARVAGFMKRYPSGLDDEIRALRAENRARIMRRYGATDAEWNDHRWQLDHIV
                     RTADELGALVTLSDEERSSIARAKVGGLPFGITPYYASLMDDEPHRRRDHAVRAQVIP
                     PADYVASMLRHREDRSHSLDFMMEHDTSPIDLVTRRYPSIVILKPYNTCSQICVYCQR
                     NWEIEDAFCDNARASDEALEAALGWIADHPAVSEVLVTGGDPLIMEDGQIDALMARLA
                     DIGHVERIRIGTRTPAVLPQRITSALADSIARYHRPGRREVAIVTHFEHPYEITDEAM
                     GAVQRFKRRGMSVYNQTVFTVENSRRFEIVALRRLLRLIGVDPYYTFNTKGKEETRKY
                     RVPMARLQQEVKEEARLAPGLARTDEPVYNVPRLGKNYIRAEQNHTLLTILPNGSRVY
                     EFHPWEKKLALVDTYIDVDVPIYDYLKELERRGEDIEEYRSIYYYF"
     gene            complement(12914..13870)
                     /locus_tag="PKJ16_02680"
     CDS             complement(12914..13870)
                     /locus_tag="PKJ16_02680"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNV45918.1"
                     /translation="MIDTRRSFLRKGALLTLSSLPLAAGCAHLEQRIERLKTSSPRTA
                     AVLWYSQTGHTRVMGRLMAQVLRAEGLTVTDADYREFDASTLPSFDLVIMGSPVYYFR
                     VPENLRAWIAKLPSLEGIPAAAFVTFGGVGHNQHNTSYELLEWLADRGAVPMRIAAFG
                     HMSSYAPTWSSGRSARILTFRDLPNEQTFEAGRDFARKTVASVRARERASIGTEVSVF
                     QPLRRLDMPWWTKLLASNHRIDPNTCIRCGKCERACPVGAVSVERNRVDSARCIFCVG
                     CVNNCPTGAMKMTYFGSEVYGYREFLKRNGITLRLPRELADG"
     gene            complement(13988..15559)
                     /locus_tag="PKJ16_02685"
     CDS             complement(13988..15559)
                     /locus_tag="PKJ16_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV45919.1"
                     /translation="MNASAQAGIEARSQRYYSFFGFGMGLFLLVLVITSFDVMGVISI
                     YICLAGATYLIGYALLTFYLARKNITSTFLKYFGTVSLFAGLTIAKFSFVFGRIGYAD
                     VLKETMTFDLYFILIIFTAVYNDRRLTIISGIMAALMYGALVAIGIVGYGLIPTDAPE
                     ANFSREHVRINIEIVKCCLLIGTGVLMNFITGSINTLLDRVRRSEAEALTRVDYQNRV
                     IDEVSTKSDHLLEVSDRQSTLEREINVNTTGQLEFAQALSRYVNDLYNLARSVSENIS
                     QQTGMAADLKSHVINLREWHDDASDYSKKVQALAGTINRSSVESSGDIAESIRRIQVI
                     SDGTQSIQEFLGIINDITDRINLLSLNAAIEAARAGEYGRGFAVVADEISKLADATSE
                     QSVEISKRLQKNIEDVKEGERYIQKSSQSFNQIIESIKTAQEYLGTIFAIIDRLNAAS
                     YELDGKVKALSDFSVSIDESSQRQSVITGEIKTRIDEMVRSCNMVIEGGRELAEISSD
                     IARLSGELKVTVAVD"
     gene            complement(15888..16955)
                     /locus_tag="PKJ16_02690"
     CDS             complement(15888..16955)
                     /locus_tag="PKJ16_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45920.1"
                     /translation="MSDKKSIIIAVAHRTGVALVDVAIDAGAVSVRASLDCTGVIALA
                     HGGGCLYALIDDAALRVISLDGRLSIVAEKKLRSAGSIAADDARVCIGADGALIVLGL
                     DLSERGRVTLIPDWPLAHDKNAHHIILHKDTAYLLDNIEQPVYVFRVDIADPALPRIV
                     ERHEWSDTYAHLAAQWLFPDEEKWIVAMTTSGMFGEEHRLYEFGPDLPEGKLQYFTNF
                     RRICAPHSESGTLEVEGRPVLGVLPGPPVWSMTRIASHNDSAITGFEYGLIKWWLDGE
                     SLEFSPELVPAPSFNVDEFFATRFVMRRQGDIVAIAPHEGALLAIAECGETARILREI
                     DLRDIGIENVFDVIIVASEGR"
     gene            complement(16945..17571)
                     /locus_tag="PKJ16_02695"
     CDS             complement(16945..17571)
                     /locus_tag="PKJ16_02695"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HNV45921.1"
                     /translation="MSEHFIPSVFFTLFLVVDPLGLIPVFVMYLQGYSASFRRRIIIK
                     ATVIAFAVSVFFVVLGDYFLKFIGVSAASFLIAGGALLFLIAIDMLFARDDRTRRPAA
                     DAAPGVEEDVSVFPLAIPMISGPGTIAALLMFSAQVRGDARGTLVIIALAAAVLAITM
                     AVMFLSAHLKRLIGETGISVIQRLMGLILSAMAVQFIVNGLKGIGIVR"
     gene            complement(17568..17885)
                     /gene="cutA"
                     /locus_tag="PKJ16_02700"
     CDS             complement(17568..17885)
                     /gene="cutA"
                     /locus_tag="PKJ16_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HNV45922.1"
                     /translation="MNEYTVIFCTVPNNDVANAIAEKVVGERLAACANIVPGLRSIYT
                     WKGAVCRDDELLLVMKTKQSLFEELRAAILSAHPYEVPEIVALPIVAGHDAYLSWITD
                     VTR"
     gene            complement(17882..18364)
                     /locus_tag="PKJ16_02705"
     CDS             complement(17882..18364)
                     /locus_tag="PKJ16_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HNV45923.1"
                     /translation="MNRITDTLVDALLTQAAASARRRAIHCFHRPEEYLQRMVNACLR
                     GTCVRPHRHKDPDKLEVFYCIRGRAAIVFFDDGGAVRDCAIIDERGPCRSTEIAPGEW
                     HSFVALADEAAFFEIIEGHYEALTHKHYAPWAPAEDDSAAAAYLDGIIDILRKRGDIT
                     "
     gene            18504..19667
                     /locus_tag="PKJ16_02710"
     CDS             18504..19667
                     /locus_tag="PKJ16_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV45924.1"
                     /translation="MLKIACAALLIVASAPKRPAGVPTRNLVQKESVIFGANSIQRLY
                     FATSSSYSRNHDGKKAIDSDRESAWVSQQGNGPHWIEIDFDTKRLMTKVVIYPGRHDN
                     YKTVKSLKLQFLYEGNWFDFSTVRLDEQQTVSCLDLFSIPWGSYRDKVEIDLGGIDAS
                     AFRIYIPAGETFGGYAAIAEIELYMGATKLQYFDDRLLGLCFPVKNGFLPDADSAYPN
                     SPRGYRGGVHVGLDVFAYHNDDSYTPIAVTRKTPILAADRGTVIRADWDYKPMTPEEW
                     KNQSAFYQKHPRTFVKRSFGGRQVWIDHGNGVVTAYNHLSGIKSGIKKGSSVGKGDVI
                     GWCGNSGLMGEAEGKNYGIHLHFEIWVDGRYLGYGMDIKDVRKYFSWIFSNRP"
     gene            complement(19685..20140)
                     /locus_tag="PKJ16_02715"
     CDS             complement(19685..20140)
                     /locus_tag="PKJ16_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45925.1"
                     /translation="MAIRTTINIRHDVYERLSLAANRLNRPRRDVLMAVVMMLMADVE
                     RFQRSFRTVRYQPDCPKESWRCYSIVLRPDENEYCVDLRKACKCSVSLIVAMAVETYL
                     DRLMAKGESGMHNYARFSSYVILRAQVDGVICWHYYWGNPYNHHNTLSK"
     gene            20233..21105
                     /locus_tag="PKJ16_02720"
     CDS             20233..21105
                     /locus_tag="PKJ16_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV45926.1"
                     /translation="MPSRIFESSRRTVAVLLSLAVLTSHCAHGHEHAQRETVECDPAS
                     IVCAAANGRMRDVRDMIARGADVNRADHRGDTALYWASFRQFADIAALLIASGARDSL
                     VFAAARGDRAAVERFFVTRVPIDRPAHDGKTALIWAACKNHIDIVTLLVDRGARINIV
                     DRDGITALGHAIASNRVSVAKLLLDRGADPNGLVEDDRRITALMYAVYNNMTGTMRMM
                     IARGVDLNARAADGRTALMEACELGREDAVRLLIDAGASIHARARNGATALSVARGNA
                     AIERLLRAAGAREN"
     gene            complement(21102..21791)
                     /locus_tag="PKJ16_02725"
     CDS             complement(21102..21791)
                     /locus_tag="PKJ16_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNV45927.1"
                     /translation="MQKYAVIGLGNFGIHIAKSLSEYGSEVTGIDISQEQLLRARNYL
                     KNAVNGDATDKAFLQTVALGEMDGVIVSVGGDMGVNVLITILLKELGMKRLIACAYSE
                     PHCAILEHLGVKDIVFPQRDFAAWFGKALSIRNVLDYVPLAGEYVVMNIQPPRSFIGK
                     NIRDLQIGARFKCQILGIKYLQGNENWNADSPEWENMKIAPTADDIIPERSVLLFLGR
                     KSDLLRIQQQD"
     gene            21967..23115
                     /locus_tag="PKJ16_02730"
     CDS             21967..23115
                     /locus_tag="PKJ16_02730"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HNV45928.1"
                     /translation="MIRWERKLAAWADAGLLTAEQRDRILDFERSAVRTNVAAYTVIG
                     LGAIIIAIGVISLIAYNWDAIPDRVKLAANFILLIAGALAAARAYGSARTIAFEIICS
                     VFFMLIGASIGLISQVFHSGGAFYEAATVWAAIALPLVLASRGTVTVHLWMTIAIIAL
                     YGQIDQSLSEALRERSTFTVVLLSAHLPAALGIAALALRGRSDEDIATLGRSLAVWAF
                     AGALVGTIFLSVPSDYGDAFSPGPWIATVLVANGALVAAACALAWKVYGRGIIVLATG
                     VALYALLAITNLRQVNPDVREVTDAALFILAWFAAAFFLFRLDYRKLFNALIVGIGIR
                     FLVVYFQVFSDLAMTGLGLIVSGLAIIGLSALYIKKRESLYALMERIK"
     gene            23112..23582
                     /locus_tag="PKJ16_02735"
     CDS             23112..23582
                     /locus_tag="PKJ16_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HNV45929.1"
                     /translation="MKKRLFVYAALAVPVAALLALAAYKAHIYHRGAEIVLPITGYDP
                     RDLLAGHYLTYRVEYGIEGLCESSRTRAGCICFDTDGNRFIDDCAADSPAECYPFVRG
                     ECRNGRFVAGIERYYIPEAKAASLDSRVRKGGAKIRIAVSPSGRSVVKDLILPE"
     gene            24028..24216
                     /locus_tag="PKJ16_02740"
     CDS             24028..24216
                     /locus_tag="PKJ16_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45930.1"
                     /translation="MESKSGSTANPDSFNNLSRFQKYAGISRDNCFLVYGGDTSGLRG
                     QAHVVSWKNMEEMHQQII"
     gene            24511..27177
                     /locus_tag="PKJ16_02745"
     CDS             24511..27177
                     /locus_tag="PKJ16_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV45931.1"
                     /translation="MDSSGMLYLNFFSVGSIIPALFHLSLAFFVLTIPGKSKATFHLG
                     MMFLFMFLFNLGYFIAASAYHPAAAFHRWLTVGVILLAEAHFNVMIINFPDNRHEKAG
                     RIFIITQYAVAIVVSLVFFIMTLKAGTVYRFDGHYWDFDAERISKVVSLFIVGFIVIN
                     IVLSVFRMIRAPKGERAIFLILLVCYLLTTVVPSVINTLSRDGAVDRETFQVSWDLFN
                     ILGFYLVGIIFVGVSRDRISIMGKLIGVSMVTFFVLFQFIAYFSLQDLERSHDDLRRK
                     DLSLAIAGTPSPELRYLAWYDPKSDALGIRDGDRGAIDPQALRIHFQNTALFESIRSL
                     PGGDGGARTAALLESSHRYFDGYRRAIIGLGAGRQSGVDYTGDQISAHLLGIRRTVYY
                     RSNKISQLPDAGFRQALARFLAGQKGDFAPFADALTRALAETRLDGKELKAEMLRYLA
                     PVNEAGARNYRIGTDGRAHFVSYLRVDPASDRVYEAGFSYRAFRHYFHPSSLKLIIML
                     AAVILVIRFGFQFYFSKTLMNAIRAFSRGVKAVDRGELDVSVPVQSEDEIGYVSHKFN
                     TMVGSIRSVVGTVTTNSHEVKKVSTDLNTASTHLSDIARELAAIVEETAAAYEEMSAS
                     FESNLNEIRAQLESSDHVKSDIERINANSGQLSGRISKLTTSINDAVAQVEAGEQTMK
                     KSVAAIEGMASYLRGIEETVNSINEVADKINLLALNAAIEAARAGEAGRGFSVVADEV
                     NKLADQTTELVKGIQGTIIEQTNRISNEISFIGGTSDIFRNVQAKIAETRAVLAETIS
                     FTDGLGSMNTEIQRKINHLSDISRNIFSFSQDQKNVIDELTRAIGSISEISQRTLERA
                     DMVKGYSRIIDMSADELAENISAFTKSGDDAK"
     gene            complement(27353..27655)
                     /locus_tag="PKJ16_02750"
     CDS             complement(27353..27655)
                     /locus_tag="PKJ16_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45932.1"
                     /translation="MDCDVCGRDIDHNYNRAKITGRYSPVAKLCFLHAEAAVQVADNG
                     TVNWLAMVHATVRLRQEEPNLFEEPDRYAEWDPLPRLVALYRAALAEYPKDLPPPT"
     gene            28051..28602
                     /locus_tag="PKJ16_02755"
     CDS             28051..28602
                     /locus_tag="PKJ16_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HNV45933.1"
                     /translation="MLIHAATKPHTFLPGAIISSSDVNENFDAAFDAINTLIPAGTVM
                     AYAGSTVPAGYLVCNGSAVSRTTYADLFAAIGTMYGSGDGSTTFNLPDYRGMFLRGHH
                     FGKSSGTTDPDYATRQMGAAQSDLIKSHQHLLPYSNGATAPSGTHATPPWQGIDSTQH
                     STVAFGGTETRPVNVSVAYIIKH"
BASE COUNT         5371 a         9032 c         8660 g         5542 t
ORIGIN      
        1 cctgcggggc cacgctgccc ttgatatcga cggacatccc gcccaccgag atattgctga
       61 tccagcagtc gtgcatcatc gcgacggcct gctcgcccat catccggcaa taggtcgcct
      121 gcacaaactc gattcgccgg tgtttccgcc gttccatgcg ccgcacctcc acttcatcat
      181 accctgtata tatggtctcg atggattctg caatcacaca ttttttttgc aaaaaatcaa
      241 ttctttttca aaaaaaatac gaccatccgt ccacgtgtcc ggattgccgg gcgaaaaatt
      301 tttatttgac tcatcgccaa atcgcgctac acagataccg atccgcgtca tggaggcccg
      361 catggacatt cagctcaacc tgtcggcatt caccgatgag cgacgatttg tgccgctcga
      421 tcccgccgcg ctctacgacg tcctcatcgt gggcgggggg ccggcggcca tgaccgcggc
      481 ggtctactgc atgcgcaagg gcgtctcaac cgggctcgtc accgaacgct tcggcggcca
      541 gatcgcggag acctcgtcgg tcgaaaatta cctgggcttc acctacatcg agggactcca
      601 gttggtggaa cggttcaccg atcaggtgaa gcagtttccg attgccgtca tcgaggggag
      661 ccgcgcgcgc gcccttgccg tggacggccc cgtgaagcgc gtcacgctcg aaaacggagc
      721 gacgctccag gcgcgcgcga tcgtcatcac caccggcaaa cactggcggc ggctgggtgt
      781 ccccggggag gcggagctca cggggcgcgg ggtggcctat tgctccacct gcgacgcgcc
      841 gctcttcgcg ggcaagtccg tcgccgtggt gggcgggggg aactccggcg tcgagtcggc
      901 gatcgacctc gcgcgcatcg ccgagcgcgt gaccctcgtc cagttcctcg acgcgctcac
      961 ggccgaccgc gtcctcgtcg atgcgctcgc gggcttcgcc aacgtggaga tcctgtttca
     1021 tcacgaggtg acgcgcatca acgggacggg cgcggtgcag tcgatcgccg tgcgcgacaa
     1081 cgcaaccggc gccgagcgcg atcttcccgt cagcggcatt ttcatcgaaa tcggcctgtc
     1141 gcccaacagc aatttcgcgc gcggcgccct cgagctcaac gagcagggcg agatcgcggt
     1201 cgattgcgcc tgccgcacca gcgcgcccgg cgtcttcgcc gcgggcgacg tgacgacggt
     1261 tccgttcaag cagatcatca tcgccgcggg ggaaggggcg aaggccgcgc tctcggcgtg
     1321 cgaatacgta ctcacgcaat cataacgcac cgatcaacac acggaggaaa acggcatggc
     1381 ggtattcaat gaaaaggtac gcggtcaact caaggacgta ctgaagggca tgatcaatcc
     1441 ggtcacgatc gccttctttt cgcaggaggt cgaatgcatg gcctgccgcg aggcccgcgc
     1501 cttcctggag gagatcgccg gtctctcgga caagctcacg ctcgtcgtgc acgatctgca
     1561 aaaggacgcc gcagaggccg ccgcgctggg catcgacaag gtgccgggga tcgcgctcct
     1621 cgacgcaaag ggcgcgccca cccgcatgaa cttcttcggc attccggggg gctacgagat
     1681 caactcattt ctgggagcat tgctcgaggc gtcggggaag cgcgaggccc tgccgggcga
     1741 tatcgccgcg cgcatcgcga caatcgctgc ggacgtgcgc atccaggtgt tcatctcgct
     1801 cacctgtccc tactgcccgg ccgcggtgag cgcagcgcac cggatcgcgc tggaaagccc
     1861 gcgggtgcgc gccgacatgg tggacagttc gatcttctcg cacctggcga tcaagtatca
     1921 ggtgagcggc gtcccgaaga cggtcatcaa cgagacgcac gagctggtgg gcgcgcagcc
     1981 gctcgggaag ctcctggata tcatcgagaa gctctgaggg tggtgccgcg gctcagagcg
     2041 cgatctgctt tttcgcctcg gccatcgtcg tcaccgggag gacgatcttc ccggcgccga
     2101 agaccgcgtc caggagctcc tgcggcgcgc tccggtactt gagggccacg atcacggtga
     2161 ccgtcttcgc cttcaggttg ggcaccgtgt atgtttcaac gacggagccc agcggcggga
     2221 tgcgatagtc cttcagcacc tcgcgcgcct tggcgaggtt cgcgaccgcc ttccccttcc
     2281 cgtcgccgaa cacggtgttg tagaccaccg cccccgcggg tatcgttccg tctcggccga
     2341 gcttgccgga cgagaagagc tcgcgcccgg cggcgtcggt gaccacgacc tcgagccaca
     2401 tctgccgcac gtcggtgagg ccggtgggga gcttatgccc ggcgccgatg ttcttcacct
     2461 cgaccttgac gatccccttt gacagctcgg catcgatgat ggtgagcgcc gcggcgttct
     2521 tgagccgttc ctcggccatg cgcgccttca cggcgtcgcc gaatttgttt gggacatagg
     2581 cgttcgcgcc cacgaagtag tgcgtgtaga tatgcgcccg ctgcgggcct ccgcccgccg
     2641 cctcgcccgg gttttgggga cgcggagtgg agccggtcgc gggaacgccg gggcgctgat
     2701 acatatggca cccctggcag ggaatccgct tgttcgaatc ggacgaatta tacgggctgc
     2761 ccagccattc ctcgtaggtg gtctcgagct tggtgccgta caccacgtgc cggacatcgt
     2821 ggcaggtccc gcagatgccc gactcggtgt ggaacttcga gtaggcagat ttgtggaact
     2881 ccgagtgcga gtccgtgaag gggccgcgct tggtccccgc attgtccatg ccgtgtccgg
     2941 gatcgagccg gatgtcgttg ttgtacattt tgcgcgcgcc ggtcgcggag tggcagtaat
     3001 cgcactggat cccctgcttg gcgatgtcgg ggacgcggtc cagatcatcc gacaccttgg
     3061 tggggttgcc ggtcacgaag cccaccggcg tgtggcactt gacgcacgat tcggcctcgg
     3121 cgatctcatc gggatccgtg aggccttttc gcaggtgttt cgcgaccgcc gtgtacaccg
     3181 gatcgaaatg cgacaggttg tgcatcgagt tcttccactg atcggccacc gccgcgtggc
     3241 agccgccgca catgtccggc cccaggaagc gcgacagctc gaagtgttgc gtgcgcccgc
     3301 aggagaacat ggcgagcgga tagacgatca tgagcgccgc accggctgcg ccggcaatgg
     3361 tccgcttcgt tccggttttc atcatcacac ccccgccgtg atgcgacagc tcactgcatg
     3421 tcgtagtcgt atatcgaaaa ccccaggagc tcgtttcgct cgtcgaaata ctcctggcgc
     3481 gtcttgttcc ccttggcgtc gtacttgtag acgcccgagc cggtgatgtt gccggcgcgg
     3541 tcgtaggtcg cgaccttcgc gagattgccc ttgtcgtcgt aggtgaattc cttcgtgaac
     3601 ttgaggccca tcccgccgcc ctcgcgctcg aagtactgca ccttgacgcg gcggcccgcg
     3661 gcgtcgtacg aggacaccat gttccacagg agcgcgctgg acgcggaata gttctggtgc
     3721 tcggcgagca tcccgcggtc attgtaggtg aacatcttgt acgaatcgag ccggccctgc
     3781 ccgtcgaagc gcgtgcgctt caccaggttg ttctgcgcgt cgtattcgtg gacgctatag
     3841 cgccgcaggg cacccgcctc gtaatacgaa tccttcacga ggttgccgtt cttgtcgaac
     3901 tcgaactggt agccggtggg cttgcccgcc gcgtcgaaat tggacttctt catgagggtg
     3961 cccgcggcgt cgtactcgaa ggtgatgtac gtgcgcagcc ggtcgttctt gtcgtaattg
     4021 gacagcttca cgatgttctt gtggaccgga tcgtactcct ggaccacgta gcttttcagg
     4081 ttgtcctggt tgtcgtagac cagcaccttg acggtatcgc cctctttttt gtagcgcttg
     4141 gtggcgccgt gcagcaggcc gtccgagcgc tggatggatt cgctgaaggt gttccccgcc
     4201 gagtcgctct cgtagaaatg atactcgcga aattttccct gccggtagta cgtttgcttg
     4261 aagatgcggt acgatccgct cgccgcgggg gtcgcctcgt cgaaccgcga cgcgtcccgg
     4321 gcgcagccgg cggccatcag cgccgcgcag gccgccgccg cgcaccgcgc cagtatatgc
     4381 ttgttcatgg tttcattctc cttcccgagt catgcgcgcc gccgtcactt gccgtcgacc
     4441 cgcttgtgca gatcgcgctc gcgctgccgc accatcttca ccgcccgggc gaacaggtag
     4501 tagaactccc ggagttcgtc gtccttgcgc aggggccgca gggtggcctt cttgccctcg
     4561 atgaagtcgc gcaggaagtc cgatatgacc atgatgggcc cggtgaactt gtggctgatc
     4621 cgtatcccga gataatacag cacgatgccc tgcaccacca cgatcacgat gatcgcgatg
     4681 agcaggatat tgttgaaccg caggatgttc tggacggtcg tgatgttgtt cttcgtgtcg
     4741 gacacgttct tcttgatcgc ggtgatgttc ttgtagtggt ccatggcgaa ttcctggatc
     4801 gccagcttct gccggggatc ggccacggag tacgagtagg tcattaggtc ctccaccaca
     4861 ttgtcctgga tggcggcgat attgtcgatc cgatggataa tgtgggagtt ctttttgatg
     4921 atgtcggaga gctgattgtg gttgtagttc acgctcaccg cgatcagggc gatgatgatg
     4981 gcgaccccga ggaacacgat ccccatgacc gacatggtgg tgcgaacctg aatcttatgc
     5041 agaatcagct tggttctctt ctttttggtg tccatatgca ttcctttcct gtgatatgcg
     5101 aattcgttcc gcccgggcgg cgcgcgcggc gccttccgaa tccggtatgc ggctgccggg
     5161 aaatactaca atcataaatt agcgcgcgca gcgcaatttt tacatgcgcg cggcgcgtca
     5221 ccggacgcgc gttccgaggt ccgtgcgcac cgacacgacg cggccgttat ccagcccgaa
     5281 gaacaattcg tcccccacgg ccgcagccga cgttgtgacg gcatgatcga agtcccgctt
     5341 ccattccagc gcccccgtcg ccgcggcaag ggcgaaaatc gtccgtccgg cgggaacgta
     5401 cacggtgtcc cgcagcgcaa cggcctcggc ctgtatcttc ccgccggatc gatagcgcca
     5461 gcgctccttt ccgttgtcgg ccgcgatggc gaataccgtc ccggcgctcg tcccggcgta
     5521 caccgtgccg cgatatggcg tcggcggcgc gagaatcgca tcgccggcta ggaatttcca
     5581 attgagcgcg cccttcttcg cggtaaggga atagacgctt cccgcgaagg agccgacata
     5641 gagcgcgccg tcggcgacaa cgggcatggc cacgatacgg ccgtccagtt cataggccca
     5701 cagctcggcg ccattggacg ccttcagggc atggagccgg tagttgttcg tcccaacata
     5761 cacgacgccg tcatgatggg cgggcgagca gtagatgccg ccatcgagcc gcttctgcca
     5821 cagttcggcc cccgacgtgg aaaacgcctg caccacgccc tggcccgtgg ccaaatacac
     5881 ccgatcgccc accacgagcg gcttcgatcc gaagagcatg gtgcccgccg ctttgcggct
     5941 ccacacgacc gcgccgcttt gcgcgttgat gcagtagagc gtgccgtcga cggtcacgac
     6001 gaagacctgc gactgctgga ccgcaggcgc cgactcgaac cgcttctgca gattggtgtt
     6061 ccacgcccgc gacccgtcct tccggaaacc cgacacccga ccgtcgttcg agccaacaat
     6121 gagaatgccg cccgccgcgg tgaccggcgt tgccacgatg ggcgccgcca tgtcggctat
     6181 tggcgcggcc cgctcctttt tcttcacgcc cgccatctcc gcgcgcagct ccagggtctg
     6241 tccatccgcg agcgtcaccg acttctggta gggctcaaac tccttcgcgg cgatctcgac
     6301 gcgcaccgcg acgcccggct tcggttccac gcggaccata tcgtgcccca ttagcttccc
     6361 gtcgatgctg atagcggcgc ccttcgccgc tgccggcgcg atcaccacta acaccgcctt
     6421 cggcttctcc ggctccaggc ggttcacgga cagcggcagc gtatcgctgg acatcacctc
     6481 cgcgcgcgaa tcgtcgatga ggcgcacccg ccagaagtac gcgcccgcct cgccgagggg
     6541 aacggtcacc cccgtgccgc tgaccagctg tcgcgtgtgc agaaccgcga agtcgcggtc
     6601 gcgcgccagc tcgaactggt agcgcccggc cattccggcc ggtttccagc tgaaggcgac
     6661 ggggaccgtc ttctccgcgc tcttgacggt gatcaccgcg cccgtggccg gggcgagcgc
     6721 cctgatcgac tcgggcgagc tcacggtgaa gcgccgcacg gccgacagca ccggctcctc
     6781 gccgggcgct ccgactccct ggacgcgcca gtagtactgg ccctcgggca gcgggcggtc
     6841 cagctcgtaa aaattaccgt tattcgaaat ggtgataacg ccctgcgaaa aaccctggtc
     6901 gcgtgcgatg gacaggcggt acgaacggat ctgggggtcc ggccgccagc tgaacatgag
     6961 ccccgccgat ttcacgacgc cggcgctgat gacgcgatcg ccggccggcg cgatgaggac
     7021 cggcggctcc agcgccttct tgcgggacac ggtgaactgg tgcacggtgc tggacccggc
     7081 atatccgccc gcggcgatct ccaccgacgt gcggactcgc cagaagtaga cgccctcgcc
     7141 gagcgagtcc accgcgatct ccgtgaggtt tgccggaaca gagcggacga ttcgcgtgaa
     7201 cgccgcgtct gcggccacct cgacggtgta gccggtcgcg agggcgttgg actcccagcg
     7261 aaagccgatg atcggcgccc gcgccacaaa ggcgaactgc gcgccgttca tgggcgtcgt
     7321 gagccgcacc ggccggtcct gcacgatggt gagcttgcgc gtctcgctgt actcgacgcc
     7381 gccgccggcg tttttcgcgc gcaggcgcca gtaatggctc cccgcgccca ggacggccgc
     7441 ggcggtcgtt ccgtcgacgc gccgcgagac gacggggcgc gagacggcgc tgtcgggggc
     7501 gatctcgaag aacacctcgg tcgcgccgct cacgggctcc caggagaagg atatcggctg
     7561 attcgcctgc tgggtcacga agaaccggtt cgcctccggc gcggcaaggc gcaggcggat
     7621 atcgtagacc ttcgcctcct tcgcgccctg cgacacgacg acccgctggt cctcttttat
     7681 ggtcttcacc tcggagccgg tctcgatctt ggcggtcccc ttggtcacgg tgagattgag
     7741 gtccttgttc tcgtccttgg tgagcttcac atcgctcttt tcgatggaga cgttcgcgtc
     7801 cttggatacg attttcacct gctggatgtc cttcgcggcg acatcgcccc ggcgcgcgaa
     7861 gatggacccg tgttcgaagt ttatgttgat ggcgccctcg gccatcgaga gcaggatcat
     7921 gctgttgtcc tcgagctgga tgtccgtgcc gtccgcgagc cggatcaccg cctcggagct
     7981 gtccgaggtg cgcacggaat cgttgttgaa cacggggacg ttctgctcga cgtcctccca
     8041 tatcacctgg gccccgtact tccgctgcgc gacgcgcttc ttgtaggtga tggtgccgat
     8101 ctggacggcg tcccccgcgt cgagccgggc ggagagatcg gcgtacagga gcgtcgagaa
     8161 ggtggcgatg atgacggcgc tcgcgcccag cgcgatcgcg tcggcgctga gggtcgacag
     8221 cctattcaat aatctcatac ttcacttctt cctcgggggc ggcgtccggc ttgcccttca
     8281 tctcgatccc caacatcgcg cgcagctcgt cgaggctctt cggggccgac gggtcgttca
     8341 tccgccccag caccgcgtag atctgctgcg gctccgactt gcccttcacc ttgatcttct
     8401 gcatcggctc gcagacgaag aggtccttca cgatcgcgta ggaatcctcg gagatgagga
     8461 tgtcggtgcc gaagggcttg ttgagcgcct cgatccgcga ggcgaggttc acggtgtcgc
     8521 cgatcaccgt gtactccatg cgctcatcgg agccgatctg ccccgcgagc accgggccgg
     8581 tgttgatccc gcacccgatc ttgatgatgg gcttcttgtc gccgccgcgc ccgcggttga
     8641 agcccatgag ctcgttgcgc atcatgagcg cgccgctgac ggcgttcgcg gtgtcgttgc
     8701 cgtgcgagat gggcgcgccc catacggcca tgatcgcgtc gccgatgaac ttgtccacgt
     8761 tgccgttggt gttgttcacg cactgcacca tccgcgtcat gtactcgttg aggaattcga
     8821 ccacctcctc cggctccagc ttctccgaga tggcggtgaa ggaccggatg tccgagaaga
     8881 agatgcaggc ggtcttgcgc tcgccgccca gcttgatctc gcccttgagc acctgctcgg
     8941 cgatctcctt gttcacgaac ttcccgaagg tgtccttgag gcgctcgcgc tcctcgaggc
     9001 cctgtcccat cgacacgaac gagcgcgtga gctggccgat ctcgtcgccg gaggtgggcg
     9061 cgatgtccac ccggaagttt ccctgctcga tctgcgcggt cgcgcccacg agccgcacca
     9121 gcggcgtggt gagcgttttg gagaagaaga acaccacgag gatcgccagg ttcaggaaga
     9181 tgatcatgat gtagatgtta cgccgctgaa tgctgtagac cgcctggaag gccttttcct
     9241 cgggaacggt cgcgacaatt ccgatgccgc tgaagccgat cttcttgaac gagcccaggt
     9301 ggtacacccc gcccgtgtcg cggtagcggg tctgctcgtt gtcgtacttg ctcttcatca
     9361 tccgctgcac aatgggcagc gcgaggtagt tgccccgcga gaggacgatc ttgctgtcgg
     9421 agtgcgcgat gatgtcgccc tggtcgttca ccatgaaggt ctcggtgatg cccgcctgcc
     9481 ggaaggcctg caggaagcgg tcgagcttga gataggcgat ggcgatggtg gccacgcgcg
     9541 tcccgtcgcg ctggaagggg atgctgatcc cgatcaccgg catcttgaag ctgtcggaga
     9601 ggttttgcac caccgcctcg ccgccgaacg agcgcgcgaa ctgcggcgcg gccgtgcggg
     9661 tgatcgcgtc gatcgccgcg ggcgtcatct ggttttcggc gaggaaggcc gcattggcga
     9721 tgctgcgcgt cacggcgatc gccgggctcc cggccgaccc ggcggcgatg cccgcgaaga
     9781 tgaagtcctt gtcgttggtg aagaagaggt cggtgaagag cttcttctgc gggaagtcct
     9841 gcagcatggt ggtgcccatg atattgagct tctccaccac ggcgaggaag tcggcgcgca
     9901 ccttgagcgc ggtgacctcc gatatcttgt ggttgctctc ctcgatgcgc agcttgttgt
     9961 cgctgcggaa aaaatagctc gccaggaaga tcatccccga gagcgccgtg acgatgatga
    10021 gcgagatgat ggagatgagc ttggtgcgga tcttcatcgt gtagatgaat tgccggaccg
    10081 gcttcgcgat gtcctcgata gtcatggtga tgccgccaat gtgtgccgtt tcttgtttga
    10141 tagaatgccc gaaacgcggc aatcagtcaa ttaaaatatc ttgggcgtca cgcaccgacg
    10201 gcgcctggca cgccggatca gactatcaaa tccagcgaag aaatacggca aacacgacaa
    10261 agccgatgcc ggcgagcgcc cagcaagccg ccgcggcgaa ggccttgttt ttcagcgaaa
    10321 cgcgatcatt gaaccaggcg atgaaggtga acggcccgct gtacgcgatg atgatgagcc
    10381 agacattggg ccagctccac cagcgatacg cccaggcgag cgcgccccac cggttcaaga
    10441 ggatctccac catcacgcag aagagtccga agcacacggg gatgactatc cggttcgaca
    10501 ggcccagtat ctttcgaccg cggtcggctg gaagcgcgtt gaaaagcgcg agcggcgcca
    10561 gggcgaacat cagcgcgatc tccacgttca atcccacgaa gatgagaaac acgctcttcc
    10621 cgctgacggt ccagagcgcc gcgtattgcg tgaaatgaag cacgagcgcg ttgaacatct
    10681 cccagatgag ctcgcccccg aagaagccga tgcccgttat cacccggtcc cagtgcccgc
    10741 tgcgaacctc gaggctgtac gcatagatga cgagggccag gaatggcacg agataccatt
    10801 cgaaggagtt cggagtacgg agattgccaa gtgcggtgat gctagcttct gtcatggggc
    10861 cttcctcgcg agcctgccgg catcaggatg ccgttgggga atcagatttc cattatatac
    10921 cgatggtaca tattaatatt atcacgtcaa tagcttttct ctgcaggtac gacatactga
    10981 ttgtgattag cataaaaaaa ttaaacctat tatgtactgt tttagatatt gttaaaaaat
    11041 gtatggacat accttaacaa atatgttatt tttccatgcc taatcagtat gacctgttaa
    11101 taaaatgaat ttcacgcgcg aaaattgcga gcggctcttt tcgggtaata ccgttatcca
    11161 gcaaatcctg aaaagcgcgc cggactaccg cacggccaaa aatcgcctgt ccacgtattt
    11221 atatgatatg gcgaacatgc tgcacatcga tctcatggcg ccgaagagcc tggaatggtc
    11281 gctccagatc aactgcatct atgcgctcag gaccatcatc tcgctgcgca gcgagaacct
    11341 gaccggctac agcatcatgg aggtgctgtg gcggctcgcc cgcgaggaat acgattccgt
    11401 cccggatgat ctctccgacg ccttcctcga tgatctgtat cacatcttca tcggcaccac
    11461 gggaaacagc cgcgtgtacg accgcgagat ctacagcgtg tcggcggacc gtcacgggcg
    11521 cgaagcggcg atcgagcggt ccgagcagct ggacgccatg gccgcgcgcg tggcgggctt
    11581 catgaagcgc tatccctcgg gcctcgatga cgagatccgc gcgcttcgcg cggagaaccg
    11641 cgcgcggatc atgcgccgct acggcgcgac cgacgccgaa tggaacgatc accgctggca
    11701 gctcgatcac atcgtccgca ccgccgacga gctgggcgcg ctggtgacgc tttccgacga
    11761 ggagcgatcg agcatcgcgc gcgcgaaagt cgggggactg cccttcggga tcacccccta
    11821 ctacgcctcg ctgatggacg acgaaccgca ccggcggcgc gaccacgcgg tgcgcgcgca
    11881 ggtgatcccg cccgcggatt acgtggcctc gatgctccgg caccgcgagg accgctcgca
    11941 ctcgctggac ttcatgatgg agcacgacac ctcgccgatc gatctggtca cgcgccgcta
    12001 cccgtccatc gtgatcctga agccctataa cacctgcagc cagatctgcg tgtactgcca
    12061 gcgcaactgg gagatcgagg acgccttctg cgacaatgcc cgcgcctccg acgaggcgct
    12121 ggaggccgcc ctcggctgga tcgcggacca tcccgccgtg agcgaggtgc tcgtgaccgg
    12181 cggcgacccg ctcatcatgg aggacgggca gatcgatgcg ctcatggcgc gccttgccga
    12241 catcggccac gtggagcgca tccgcatcgg cacgcgcacg ccggcggtgc tgccgcagcg
    12301 gatcacctcg gcgctggccg acagcatcgc gcgataccat cgaccggggc ggcgcgaggt
    12361 cgcgatcgtc acccacttcg agcatcccta cgagatcacc gacgaggcca tgggcgcggt
    12421 gcagcgcttc aagcggcgcg gcatgtcggt gtacaaccag accgtgttca cggtggagaa
    12481 cagccggcga ttcgagatcg tggcgctcag gcgccttctg cgcctcatcg gcgtggaccc
    12541 gtactatacc tttaacacga aaggaaaaga ggaaacgcgg aagtaccggg tgcccatggc
    12601 tcgcctgcag caggaggtga aggaggaggc gcgcctcgcg ccgggactgg cgcgcaccga
    12661 cgagcccgtg tacaatgtcc cgcgcctcgg gaagaactac atccgcgccg agcagaacca
    12721 taccctgctc accatccttc cgaacggcag ccgcgtgtac gagttccatc cctgggagaa
    12781 gaagctcgcg ctcgtggaca cctatatcga cgtggacgtg cccatctacg actacctcaa
    12841 ggagcttgag cggcgcggcg aggacatcga ggagtaccgg agcatctact attacttctg
    12901 atcgcgcggt ccgtcacccg tccgcgagct cgcggggcag gcgcagggtg atgccgttgc
    12961 gcttcaggaa ttcacgatac ccgtagacct ccgagccgaa ataggtcatc ttcatggctc
    13021 ccgtggggca gttgttcacg cagccgacgc agaagatgca gcgcgcgcta tccacgcgat
    13081 tgcgctccac cgagacagcg cccacgggac acgcccgttc gcacttcccg caccggatgc
    13141 aggtgttcgg gtcgattcta tggttactcg ccaacagctt ggtccaccag ggcatatcca
    13201 gcctcctgag gggctggaat acgctcacct cggtgccgat gctggcgcgc tcccgggcgc
    13261 gcacggaggc gacggtcttt cgcgcgaaat cgcgaccggc ctcgaaggtc tgttcgttcg
    13321 gcaggtcgcg aaacgtaagg atgcgcgccg accggccgct ggaccaggtg ggcgcgtagc
    13381 tggacatatg gccaaaggcg gcaatgcgca tggggacggc cccgcgatcg gcgagccatt
    13441 cgagaagttc gtacgaggta ttgtgctgat tgtgccccac gccaccgaac gtaacgaacg
    13501 ccgctgcggg gatcccctcg agcgagggga gcttcgcgat ccaggcgcga aggttctccg
    13561 gcacccggaa gtagtagacc ggcgagccca tgatgaccag atcgaaggac gggagcgtcg
    13621 atgcgtcgaa ctcgcgatag tcggcgtccg tgaccgtgag cccctcggcg cgcaggacct
    13681 gcgccatcag ccgccccatc acccgcgtgt ggccggtctg gctgtaccag agcacggccg
    13741 ccgtgcgcgg cgacgatgtt ttcagccgct cgatgcgctg ctcgaggtgc gcgcagcccg
    13801 cggcgagcgg cagcgaggag agcgtgagaa gcgcgccttt ccgcaagaat gaacgtcggg
    13861 tatcgatcat ggctttctcc atgggaacgc attgtgcgtc ggggcgcctg atccgcaagc
    13921 ggaatattcc gattgcatac gagtgccccg ccactggcag tgcggcgtcc gacgaggacg
    13981 acacgcccta atcgaccgcc accgtcacct tgagctcgcc ggagagccgg gcgatgtcgc
    14041 tggagatttc ggccaactcc cgtcccccct cgatcaccat gttgcagctt ctgaccatct
    14101 cgtcgatgcg cgtcttgatc tcgccggtaa tgacggactg ccgttgcgac gactcgtcga
    14161 tgctcacgct gaagtcgctg agcgccttga ccttgccgtc gagctcatag gaggccgcgt
    14221 tgagccggtc gatgatcgcg aagatcgtcc cgaggtattc ctgggcggtc ttgatgctct
    14281 cgatgatttg attgaaggac tgcgacgact tctggatata gcgctcgccc tccttcacgt
    14341 cctcgatgtt cttctgcagc cgtttcgata tctccaccga ttgttcgctc gtggcgtcgg
    14401 cgagcttcga gatctcatcg gccacgacgg cgaacccgcg cccgtactcg cccgcccgcg
    14461 ccgcctcgat ggccgcgttg agcgagagca ggttgatccg gtcggtgatg tcgttgatga
    14521 tgccgaggaa ttcctgaatc gactgcgtcc cgtcggatat gacctggatg cgccggatcg
    14581 actcggcgat atcgccggac gattctacgg agctccggtt gatggtgccg gcgagcgcct
    14641 gcaccttctt ggaatagtcg gacgcgtcgt cgtgccactc gcgaaggttg atgacatggc
    14701 tctttagatc tgcggccata cccgtctgct ggctgatgtt ctcggacacg ctgcgcgcga
    14761 gattatacag atcattgaca tagcgcgaca acgcctgcgc gaattccagc tgaccggtgg
    14821 tgttcacatt gatctcccgc tcgagcgtcg attgccgatc ggagacttcg aggaggtgat
    14881 cggacttcgt cgacacctcg tcgatcaccc gattctggta gtcgacccgc gtgagcgcct
    14941 ccgcctcgga gcggcgcacc cgatcgagga gcgtattgat gctgccggtg atgaagttca
    15001 tgagcacccc ggtgccgatg aggagacagc atttcacaat ctcgatgttg atccgcacgt
    15061 gttcgcgcga gaagttcgct tcgggagcgt cggtcgggat caggccatac ccgacgatgc
    15121 cgatcgcgac cagcgcgccg tacatcagcg ccgccatgat gcccgaaatg attgtgagcc
    15181 ggcggtcgtt gtacacggcc gtgaagatga tgagaatgaa atacaggtcg aaggtcatcg
    15241 tttccttgag aacgtcggcg tacccgatgc ggccgaagac gaagctgaac ttcgcgatcg
    15301 tgaggccggc aaacagcgag actgtcccga aatacttaag gaacgtcgag gtgatgtttt
    15361 tccgcgccaa gtaaaacgtc aatagtgcgt agccgatgag ataggtcgcg cccgccaggc
    15421 agatatagat ggatatgaca cccatgacat cgaacgacgt gatcacgagc acgaggagaa
    15481 aaagccccat cccgaacccg aagaaactgt aatagcgctg cgatcgcgcc tcgataccgg
    15541 cctgtgccga tgcgttcata ccgacctctt tgcgtcctgt ctatttcatc aatccacctt
    15601 ggcgaactgt cgatccgttg atcccgcaac gcagttttcc cgacagagac cgcttcgctt
    15661 gcagtgaaaa cgattacgca atgcaaacaa catagaccac aagactataa ttgcgtcatc
    15721 atccgtcaac catttctgcc ggtgtgatcg aagcacggga agcatcatga taataccgaa
    15781 aaatcttgag cggcgtcgtt acgattgccg cgaccaggat ggcgatgaac ctttccatat
    15841 cggtcattac cggcatttgg gacccagatg cgcccggaac ggagtcgtca tcgaccctca
    15901 gatgccacaa taatcacatc aaacacattt tcgatgccga tgtcccgcag gtcgatttca
    15961 cgcaggatgc gtgccgtctc gccgcattcc gcgatcgcga ggagcgcgcc ctcgtgcggc
    16021 gcgatcgcga cgatatcgcc ctgccgccgc atgacgaagc gcgtcgcgaa gaactcatcg
    16081 acattgaacg acggcgccgg aacgagctca ggagagaact ccagcgactc gccgtcgagc
    16141 caccatttga tgaggccgta ctcgaaaccg gtgatcgcgg agtcgttgtg ggaggcgatg
    16201 cgcgtcatcg accacaccgg cggcccgggc aagacgccga gaaccggacg gccctcgacc
    16261 tcgagcgttc ccgattcgga atgcggcgcg cagatgcgac ggaagttagt gaagtactgc
    16321 agcttcccct cgggaaggtc gggaccgaac tcatagagac gatgctcctc gccgaacatg
    16381 ccggaggtgg tcatcgccac aatccatttt tcctcgtccg ggaagagcca ctgcgctgcg
    16441 aggtgcgcat aggtgtcgga ccactcgtgt cgctcgacga tgcgcgggag cgcgggatcg
    16501 gcgatatcga cacggaagac gtacaccggc tgctcgatat tgtccagcag atacgccgtg
    16561 tctttgtgca gtatgatgtg atgcgcgttc ttgtcgtgcg caagcggcca atcggggatg
    16621 agcgtcacgc gccctcgttc ggagagatcg agaccgagca cgatgagcgc gccgtccgcg
    16681 ccgatgcaca cgcgcgcatc gtccgcggcg atggatccgg cgctgcgaag ctttttctcg
    16741 gcgacaatcg acaaacggcc gtccaggctg atgacgcgca gggcggcgtc atcgatgagc
    16801 gcgtagaggc atccgccgcc gtgggcaagc gcaatcacac cagtgcagtc gagtgatgcg
    16861 cgcacggaaa cggcgcccgc gtcgatggcg acatcgacga gcgcgacgcc cgttcggtgg
    16921 gcgacggcga tgatgatcga ttttttatcg gacaattccg attcccttga gcccgttcac
    16981 gataaactga acggccatcg ccgacaggat gagccccatg agccgctgga tgacggagat
    17041 gcccgtctcg ccgatgagcc gcttgaggtg cgcggagagg aacatgaccg ccatcgtgat
    17101 cgcgaggacc gccgcggcga gggcgatgat gacgagcgtc ccgcgcgcgt cgccccgcac
    17161 ctgcgcgctg aacatgagga gcgcggcgat ggtgccggga ccgctgatca tggggatggc
    17221 gagcggaaac accgacacgt cctcctcgac gccgggggcc gcgtccgccg ccggccgccg
    17281 ggtgcggtcg tcgcgcgcaa agagcatgtc gatggcgata agaaagagga gcgccccgcc
    17341 ggcgatgaga aacgacgccg cggagacgcc gatgaatttg aggaaataat cgcccagcac
    17401 gacgaagaag accgataccg cgaaagcgat cacggtcgcc ttgatgatga tgcgccgacg
    17461 gaaggacgca ctgtacccct gtagatacat cacaaacacc gggatgagcc ccagcggatc
    17521 gaccaccagg aagagcgtga agaacaccga ggggatgaag tgctcgctca tctcgtcacg
    17581 tcagtgatcc acgaaaggta ggcgtcgtgc ccggcgacga tgggcagggc gacgatctcc
    17641 gggacctcgt aggggtgcgc ggacaggatc gccgcgcgca attcctcgaa aagcgattgc
    17701 tttgtcttca tcaccagcag cagctcatcg tcccggcaca ccgcgccctt ccaggtgtag
    17761 atggaccgca ggccgggaac gatgttcgcg cacgcggcga gcctctcgcc caccaccttc
    17821 tcggcgatcg cgttcgcgac atcgttgttg ggcaccgtgc agaaaatcac cgtgtattcg
    17881 ttcatgtgat gtcacctcgt tttcggagaa tgtcgatgat gccgtccagg tacgcggccg
    17941 cggccgaatc atcctccgcc ggcgcccacg gcgcgtagtg cttgtgcgtg agcgcctcat
    18001 aatggccctc gatgatctcg aagaacgccg cctcgtccgc gagcgcgacg aacgagtgcc
    18061 actcgcccgg cgctatctcc gtcgagcggc agggaccgcg ctcgtcgatg atcgcgcagt
    18121 cgcgcacggc gccgccatca tcgaaaaaca cgatggccgc ccggccccgg atgcagtaga
    18181 acacctcgag cttgtcgggg tccttgtgtc ggtgcggacg cacacaggtg ccgcgcaggc
    18241 aggcgttgac catccgctgc aggtattctt ccggccggtg aaagcagtgg atggcgcgcc
    18301 gccgcgctga ggccgcggcc tgcgtcagca gcgcatcgac cagcgtgtcg gtgatccggt
    18361 tcatggttcc gatagcgtga ggccggctgc gaataatggc aataaaaatt caactcacgc
    18421 cggtcgaaat tggatttaca ccgtatatgc gctttgcaac ggtgttcccg tgccgcacac
    18481 acctgaatgt cggagtattg cccatgctca agatcgcctg cgcagcgttg ctcatcgtcg
    18541 cctcggcccc gaaacgcccg gccggcgttc ccacgcgcaa tctggtgcaa aaggaatccg
    18601 tcatcttcgg cgccaactcg atccagcgcc tttacttcgc gacgagctcg tcgtacagca
    18661 ggaaccacga cggcaagaaa gcaatcgaca gcgaccgtga gtcggcctgg gtctcccagc
    18721 agggcaatgg cccccactgg atcgaaatcg acttcgacac caagcggctg atgaccaagg
    18781 tcgtcatcta tcccggtcgc cacgataatt acaagaccgt caaatcgctc aagctccagt
    18841 tcctctatga gggaaactgg ttcgatttct cgaccgtccg cctcgacgaa cagcagaccg
    18901 tctcctgtct cgatctgttt tccatcccct ggggctcgta ccgcgacaag gtcgagatcg
    18961 atctcggtgg gatcgatgcg agcgcctttc gcatctacat ccccgcaggc gagaccttcg
    19021 gcggctatgc cgccatcgcc gagatcgagc tctacatggg ggccacaaaa ctgcagtact
    19081 tcgacgaccg gctccttggc ctgtgctttc cggtgaaaaa cggcttcctg cccgatgcgg
    19141 attccgccta tcccaattcg ccgcgcggct atcggggcgg cgtacacgtg ggactggacg
    19201 tgttcgccta tcacaacgac gattcgtaca caccgatagc ggtgaccagg aaaacgccga
    19261 tcctcgccgc cgaccgtggg accgtcatcc gcgccgattg ggactacaag cccatgacgc
    19321 ctgaggagtg gaagaaccag tcggcgttct accagaagca tccccgcacc ttcgtgaagc
    19381 gctccttcgg cggacgccag gtatggatcg accacggcaa cggcgtggtg accgcataca
    19441 atcacctctc gggaatcaag agcggcataa agaagggctc ctcggtcggc aaaggcgacg
    19501 tgatcggctg gtgcggcaac tcagggctca tgggcgaggc cgaggggaag aactatggga
    19561 tccatctcca cttcgagata tgggtggatg gccgctatct gggttacggc atggacatca
    19621 aggacgtgcg gaagtacttc tcgtggattt tctcaaaccg tccgtaagtt cgatcgagcg
    19681 aaggttactt cgatagggtg ttgtggtggt tatatgggtt tccccagtag tagtgccagc
    19741 agatgacccc atcgacctgc gcacggagaa tgacatagct ggagaatcgt gcatagttat
    19801 gcataccact ttcacccttc gccatcagac ggtccagata ggtttccaca gccatcgcca
    19861 ctatcagcga aacggagcat ttacacgcct tgcgcaaatc aacgcaatac tcgttctcat
    19921 ccggccgcag cacaatcgag taacaacgcc agctctcctt cgggcaatcg ggctggtagc
    19981 gaacagtccg aaaagaccgc tgaaaccgct cgacatcggc catgagcatc atcacaaccg
    20041 ccatgagcac gtcccggcgg ggacggttga gcctgttcgc ggccagtgag agccgttcgt
    20101 agacatcatg ccgaatattg atcgtcgttc gtatcgccat tgtcacctcc gtgggcgccc
    20161 tgcatgcgtc ctataggtga tacgaacgcc gaccgacata catgcagtat ttcccattcg
    20221 gagtgcgcga aaatgccatc acgaatattt gaatcatccc ggcggaccgt cgcggttctt
    20281 ctctcactcg cggttctcac cagccattgc gcgcacggcc acgaacacgc ccagcgcgaa
    20341 accgtggagt gcgacccggc atccatcgtg tgcgccgccg cgaacggccg catgcgcgac
    20401 gtgcgcgaca tgatcgcgcg cggggccgac gtgaaccgcg cggaccaccg cggcgacacc
    20461 gcgctctact gggcctcgtt ccggcagttc gccgacatcg cggcgctgct catcgccagc
    20521 ggcgcgcgcg actcactcgt ctttgcggcg gcgcggggcg accgtgcggc cgtggagcgc
    20581 tttttcgtaa cgcgcgtccc catcgaccgg ccggcgcacg acggcaagac cgcgctcatc
    20641 tgggcggcct gcaaaaacca catcgacatc gtgacgctgc ttgtggatcg gggtgcgcgg
    20701 atcaacattg tcgatcgcga cggcataacg gcgctcgggc acgccatcgc ctcgaaccgc
    20761 gtatcggtgg cgaaactgct cctcgatcgc ggggccgatc cgaacggact cgtcgaggat
    20821 gaccgccgca ttacggcgct catgtatgcg gtgtacaaca acatgaccgg cacgatgcgg
    20881 atgatgatcg cgcgcggcgt tgacctgaac gcaagggcgg cggacggacg caccgcgctc
    20941 atggaggcct gcgagctggg gcgggaagat gccgtacgat tgctgataga cgccggggcg
    21001 tcgatacacg cgcgggcgcg caacggcgcg acggcgcttt ccgtcgcacg gggcaatgcc
    21061 gccatcgagc ggttgctccg cgcagcgggc gcgcgggaaa attagtcctg ctgctggatg
    21121 cgcagcaaat cggacttcct tcccagaaac agaagcaccg agcgctcggg gatgatatca
    21181 tctgcggtgg gggcgatttt catattctcc cactcggggc tgtcggcgtt ccagttctcg
    21241 tttccctgca ggtacttgat ccccaggatc tggcacttga agcgcgcacc gatctgcaga
    21301 tcgcgtatgt ttttcccgat gaagctgcgc ggcggctgga tgttcatcac cacatactcc
    21361 cccgcaagcg gaacgtagtc gagcacgttc cgtatggaga gcgccttgcc gaaccaggcc
    21421 gcgaaatcgc gctgcgggaa gacgatgtcc ttcacgccga ggtgctccag gatcgcgcag
    21481 tgcggctccg agtaggcgca ggcgatcagg cgcttcatgc ccagctcctt gaggagtatg
    21541 gtgatgagca cgttcactcc catgtcgccc ccgacgctca cgatcacgcc gtccatctca
    21601 cccagcgcaa ccgtctgcag aaacgccttg tccgtcgcgt cgccgttgac cgcattcttc
    21661 aggtaattgc gcgcccgaag cagctgttcc tggctgatat cgatccccgt gacctcgctc
    21721 ccgtactccg agagcgactt ggcgatatgg atcccgaaat tccccaaccc gatgaccgcg
    21781 tatttttgca tgatcgacct ccgacccccg gcgataacgg cgcgctgcgc cttcgttgcg
    21841 cgcgaacgcg aaagactacc cgcgaccgca tcgcgagtca agcggaaaag gtcccatcgc
    21901 gcctttcgcc tcgcactgga gcaattaatg gttgcacatc cgttaaaata catacagtgt
    21961 gccggtatga ttcgctggga gagaaaactt gcggcatggg ccgacgcggg cctgctcacc
    22021 gccgagcagc gcgaccgcat cctggacttc gagcgctcgg ccgttcgtac aaacgtcgcc
    22081 gcctacaccg tcatcggcct gggcgcgatc atcatcgcca ttggcgtcat ctccctgatc
    22141 gcgtacaact gggacgcgat cccggaccgg gtcaaactcg ccgcaaactt cattctcctg
    22201 attgccggtg cgcttgctgc ggcgcgcgcg tacggatcgg cccggacaat cgcctttgaa
    22261 atcatctgct ccgttttctt tatgttgata ggcgcctcga tcggactcat ctcgcaggtt
    22321 tttcattccg gcggcgcatt ctacgaggcg gccaccgtct gggcggcgat cgccctcccg
    22381 ctcgtgctcg cgagccgcgg cacggtgacg gtccacctct ggatgacgat cgcgatcatc
    22441 gcgctctacg ggcagatcga ccagtcgctc tcggaggccc tccgcgagcg ctccaccttc
    22501 accgtggttc tcctctcggc gcaccttccc gccgcgctgg ggatcgcggc tcttgccctc
    22561 cggggacggt ccgacgaaga tatcgcgaca ctggggcggt cgctcgcggt ttgggcattc
    22621 gcgggcgcgc tcgtcggaac catcttcctg tccgttccca gcgattacgg cgacgcgttt
    22681 tcgccgggtc cctggatcgc gacagtgctc gtcgcgaacg gcgcgctcgt cgcggcggcc
    22741 tgcgccctcg catggaaggt gtacgggcgc ggcatcatcg tcctcgcgac cggcgtcgcg
    22801 ctctatgcgc tcctggcgat cacgaacctc cgccaggtga atccggacgt acgcgaggta
    22861 accgacgccg cgctgtttat cctcgcatgg ttcgccgcgg ccttctttct gtttcgtctc
    22921 gattaccgca agctcttcaa cgccctcatc gtcggcatcg gcatccgctt cctggtcgtg
    22981 tatttccaag tgttcagcga cctggccatg acgggtctcg gcctcatcgt ctcggggctc
    23041 gcgatcatcg ggctgagtgc gctgtacatc aaaaaacgcg aatcgctgta cgccctcatg
    23101 gagcggatca aatgaagaaa cgcctgttcg tctacgccgc gctggccgtt cccgtcgccg
    23161 ccctgctcgc gcttgcggcc tacaaggcgc acatctatca tcgcggcgcg gagatcgtgc
    23221 tgcccatcac cggctatgac ccccgcgacc tactggcggg gcactacctc acctatcgcg
    23281 tcgagtacgg gatcgagggc ctctgcgagt cctcccgcac gcgcgcgggc tgcatctgct
    23341 tcgatacgga cggaaaccgc ttcattgacg attgcgccgc ggactcgccc gcggaatgct
    23401 atcccttcgt caggggcgag tgccgcaacg ggcgtttcgt cgcggggatc gagcgctact
    23461 acatccccga ggcgaaggcg gcctcgctcg acagccgcgt gcgaaagggc ggcgcgaaaa
    23521 tccgcatcgc cgtgagcccg tcgggccggt cagtggtgaa ggacttgatt ctgccggagt
    23581 gagaggcaac tcatgatctt ccggttgatt ctttccgatt accatggata atacgcttct
    23641 tcgtgtttgg ctcgtcatca cgctttatct cttcaatgac gcctgttgtg atgctataaa
    23701 tgggcgtaat tacgtgacat gataaatttc acataacaga aatcatatcc ccatacggga
    23761 gcctctaata attacaacta acgcatcaat aaacatccga aactgtaatt ttacgacacc
    23821 ggccttgccg cggcgttact gggcattcgc gcacccgggg tgctcgccct tatccatctc
    23881 cgcggtaaga tctttgagaa ttccatcatc tccgaataca tcaagcattc gttccacagc
    23941 tggtgtcacc cgttctgcta tttctagcgc gataacaccg gcaatgaaat cgatccgctc
    24001 gttgaacggg gcggatcgct atatgccgtg gaaagcaagt cgggctcgac cgcaaatccc
    24061 gattccttca acaatctgtc tcggtttcaa aagtatgcgg ggatctcccg ggacaactgc
    24121 tttttggtgt acggcggcga tacctccgga ctccggggac aggcgcatgt tgtaagctgg
    24181 aagaatatgg aagagatgca ccagcagatc atttaatcgc cggcaccaac cccaccgtac
    24241 aatgcgttca ccacgaaggg agggtgcacg ctatcgccta tcgctcgtcc ggacgatcac
    24301 cgggcgcacc acgaaaaaat tgttgaataa tggccaatga aattgggaga tggatgcggg
    24361 tgacgttgtt cgacgggcgg cgcgtatcgg ccgcaatcaa catatataca ttcacacaca
    24421 tcgctttttt gcgagaagcg aagcgttttt ccgctggtta gccacggtgt ccattgaggt
    24481 ctgattatcc tttcataagg gagttttgcc atggactcgt ccggcatgct gtatctgaat
    24541 ttcttttccg tgggatccat catccctgcg ctgttccatc tttccctggc gtttttcgtg
    24601 ctgaccattc ccggcaagtc caaggcgacc ttccatctgg ggatgatgtt cctgttcatg
    24661 ttcctcttca acctgggata cttcatcgcg gcatccgcct atcaccccgc ggcggccttc
    24721 catcgctggc tcaccgtcgg cgtgatcctc ctggcggagg cccatttcaa tgtgatgatc
    24781 atcaacttcc ccgacaaccg ccacgagaag gcggggagga tcttcatcat cacgcaatac
    24841 gccgtggcga tcgtcgtatc gctggtcttc ttcatcatga ccctgaaggc cggaacggtg
    24901 taccgcttcg acgggcatta ctgggacttc gacgcggagc gcatcagcaa ggtcgtctcc
    24961 ctgttcattg tcggtttcat cgtcatcaac atcgttctgt ctgtttttcg gatgatccgg
    25021 gccccgaagg gggagcgcgc gattttcctc atcctcctcg tgtgctatct gctcacgacc
    25081 gtcgtcccct ccgtcatcaa caccctcagc cgcgacggcg cggttgaccg ggagaccttt
    25141 caggtgtcct gggacctgtt caatattctc ggcttctacc ttgtcggtat catcttcgtc
    25201 ggcgtttcgc gggaccggat cagcatcatg gggaagctca tcggcgtgag catggtcacc
    25261 ttcttcgtcc tgttccagtt catcgcctac ttctcgctgc aggacctcga gcgctcgcac
    25321 gacgacctgc gccgaaagga cctgtccctg gcgattgccg gaacgccgtc gcccgagctc
    25381 cgatatctcg cctggtacga tccgaagagc gatgcactcg gcattcgcga cggggaccgg
    25441 ggggcgatcg acccgcaggc gcttcggatc cattttcaga acacggcgct cttcgagtcg
    25501 atccggtccc tccccggcgg tgacggcggg gcgcggacgg cggcgctctt ggaatcgagc
    25561 catcgctatt ttgacggata ccgccgcgcc atcatcggcc tcggcgcggg tcgacaatcc
    25621 ggcgtagatt acaccggcga tcaaatctcc gcgcacctcc tggggatcag acggaccgtc
    25681 tattatcgct ccaacaagat ctcgcagctt ccggacgccg gttttcgtca ggcgctcgcc
    25741 agattcctcg ccggacagaa gggcgatttc gcccccttcg ccgacgcgct cacccgcgcg
    25801 cttgccgaga cgcgcctcga tggaaaggag ctcaaggccg agatgcttcg gtatctcgcg
    25861 cccgtgaacg aggccggcgc gcggaactac cgcatcggga cggacggccg cgcgcatttc
    25921 gtgtcgtacc tgcgtgtcga tcccgcgtcg gatcgggtgt acgaggctgg cttttcgtat
    25981 cgggccttcc ggcactattt ccatccctcg tcgctcaagc tgatcatcat gctcgccgcg
    26041 gtgatactcg tgatccgctt cggcttccag ttctatttct cgaaaaccct catgaacgcc
    26101 atccgggcgt tttcacgcgg cgtcaaggcg gtcgatcggg gcgagctcga cgtgagcgtc
    26161 ccggtgcagt ccgaggacga gatcggctac gtgtcgcaca agttcaacac aatggtgggg
    26221 tcgatccgca gcgtggtggg cacggtgacc accaactcgc acgaggtcaa gaaggtgagc
    26281 accgacctca atacggcgtc gacgcacctc tcggacatcg cccgggagct cgccgcgatc
    26341 gtcgaggaga cggccgccgc ctacgaggag atgtccgcct cgttcgagtc gaacctgaac
    26401 gagatacgcg cccagctgga aagctcggac cacgtcaaaa gcgacatcga gcggatcaac
    26461 gcgaacagcg gccagctctc ggggcgcatc tcaaagctca cgacgagcat caacgacgcc
    26521 gtcgcgcagg tcgaggcggg cgaacagacg atgaagaaat cagtcgcggc catcgagggc
    26581 atggcctcgt acctgcgggg catcgaggag accgtcaact cgatcaacga ggtcgccgac
    26641 aagatcaatc tgcttgcgct gaacgccgcg atcgaggcgg cgcgcgcggg cgaggcggga
    26701 cgcggctttt ccgtggtggc cgacgaggtg aacaagcttg ccgaccagac cacggaactc
    26761 gtcaagggca ttcagggcac catcatcgag cagacgaacc gcatctcgaa cgagatcagc
    26821 tttatcggcg gcacctcgga catcttcagg aacgtgcaag cgaagatcgc cgagacgcgc
    26881 gccgtcctcg ccgagacgat cagcttcacc gacggcctgg gatcgatgaa caccgagata
    26941 cagcgaaaga tcaatcactt gagcgacatc tcccggaaca tcttttcctt ctcgcaggac
    27001 cagaagaacg tgatcgacga gctcacgcgc gcgatcggct ccatcagcga gatctcccag
    27061 cggacgctcg aacgcgcgga catggtgaag ggctactcgc gcatcatcga catgagcgcc
    27121 gacgagttgg ccgagaacat cagcgccttc actaaaagcg gcgacgatgc gaagtgaacc
    27181 acgaacgcct aggagaccgc atgagcgaaa cgctcgcgag gtgtaacggt gcccatttcg
    27241 atggtcgccg caccggtcag cgaggcccgg gagacgaata tcctcgtcat gtcgaatctg
    27301 ccggggtgag ggcgtgggcg tcaatcgctg aactgcattc ggcgcgcccg cctcatgtgg
    27361 gcgggggaag gtccttcggg tactccgcca gggcggcccg gtacagcgcc acgaggcgcg
    27421 gcagcgggtc ccactcggca tagcggtccg gctcctcgaa aaggttcggc tcctcctggc
    27481 gcaggcggac ggtggcgtgc accatggcca gccagttcac ggtgccgtta tccgcgactt
    27541 gtacggcggc ctccgcgtgg aggaagcaca gcttcgccac gggcgagtag cgccccgtga
    27601 tcttggcccg gttgtagttg tggtcgatgt ccctgccgca gacgtcgcag tccatggtct
    27661 caccctttga cgaccgtgat tgtcccgatt gattatgcgc gtagtgcgcg atggattcga
    27721 atacaataag accaacgtcc tgtcaatgaa accgtcagcg tttttccgat caggcgacac
    27781 tccccctgcc gaaataaatt caaatgccgg actggaattt catggtttac aggcaacgta
    27841 aatcatgcac catagcgttt aaagcaccgg acatcgagga catgaaaggc aataatgcga
    27901 tgctcgatgg gttcgcgagt ttaatcacac aaggataccg ccatgcaaat aattatcaat
    27961 atcgacgaga aaatatttca tcgtttgaaa aagctattta ccgtacgtcg aatagtatgg
    28021 gcgattgcac tctgtgccgc aagcacgtca gtgttgatcc atgcggcgac gaagccccac
    28081 acctttctcc ccggcgccat catatcctct tccgatgtaa acgaaaactt tgacgccgca
    28141 ttcgacgcga taaacacgct cattcctgcc ggcaccgtca tggcctatgc cggttcgacc
    28201 gttcccgccg ggtatctcgt ctgcaacggg tccgccgtca gccggacaac ctatgccgat
    28261 ctctttgccg cgattgggac catgtacggc agcggtgacg gctcgacgac attcaatctc
    28321 ccggattacc ggggcatgtt cttgcgcggg caccatttcg ggaaaagctc gggaaccacc
    28381 gatcccgatt acgcgacgcg acagatggga gccgctcagt ccgatctgat caagtcccat
    28441 caacacctgc tgccgtattc caacggagcc accgcgccct caggaaccca tgcaacaccg
    28501 ccatggcagg gtatcgattc gacgcagcat tcgacggtcg cctttggcgg caccgagacg
    28561 cgtcccgtga atgtgagtgt cgcgtatatc atcaaacact aataa
//
LOCUS       DAORHF010000017        28332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1016_length_28332_cov_4.877674, whole genome shotgun sequence.
ACCESSION   DAORHF010000017 DAORHF010000000
VERSION     DAORHF010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28332
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1016_length_28332_cov_4.877674"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PKJ16_02760"
     CDS             <1..787
                     /locus_tag="PKJ16_02760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV45934.1"
                     /translation="PIIGWRSNIEFLQLDRMREFFTRHYVPARMSIAIVGKQNPAETL
                     AVLERSFGRLPKRPEPMPAAIREPAQNGERRFEVFFDANPSLVMGWHKPTLPAKADFA
                     CEVIASLLSDGRSSRLYRRLVLERKIASSVSAWNGFPGSRYDNLFVVMAAPQGSHTLP
                     EIEAAVLDEIARLAGDLRDDELERILNRIETKAILGLDSNMGIASLISHYQTIMKDWR
                     FATSYLDDMRRITRADVVDAINTYLVAKNRTVGYLLRTRDQKK"
     gene            823..2235
                     /locus_tag="PKJ16_02765"
     CDS             823..2235
                     /locus_tag="PKJ16_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV45935.1"
                     /translation="MNMIIRGKRFYGRILAVATACVMMMIPLATGTAQQATMELPRVQ
                     SWKTTNGIRVVYLEDRLPQLTVVATIGFGTLYENERNAGIADLLARTLNLAGSKRYPG
                     TAMHEAIESVGGTIGVSSSWEGITVTVRVLERHAARAFDVLGDLVTNPNWDARKLEDA
                     RALLIETIKRKQDRPFELGYEALREVVFAGKGYGAIAREKTVRACTLDDLTSVWRTYA
                     VAGNTLLGVSTSIPAPAVRRHAEVLRGVPAGDATDYAIDAAAVRASIATNAGVIYLVP
                     KDIPQATILIGTVAPDIASPNTSALRVMNYILGEGSFNSRLMEEVRVKRGLAYSVQSV
                     IGFRRNAGIFFAMAQTNADQATVTLEVMRENIARMSREPVSGEELVWARESIANSFVF
                     EFDTSLAVVQKYLYLRYNRLPDSYLTDYIPRIRAISATRIQDEARSLFGGGTVTVVVG
                     RRDMAPALGKFGRVVVLGDR"
     gene            complement(2238..3164)
                     /locus_tag="PKJ16_02770"
     CDS             complement(2238..3164)
                     /locus_tag="PKJ16_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45936.1"
                     /translation="MKTKFIKIRAISEIDPSKITVYDLNNRYIDPEGRMYGLRYNRRD
                     RKIEIIRIIRTPANSAGFYQQKVVESKRSGKSSRPESESTPASPDTISDIFERADAPI
                     DETAIGDADGADAAVSYVLDGFDPDDVVNKALELLKTHKGRLHGMMINISNTQISSKV
                     SREIGTSIETALRNLDLDGIQKIDKVMGLNKELVSFPRSVQYYIARLDTQTKDIVEAF
                     GSQDEQMRFIFLYEMYWALVSMYKSLAAIVNQTLVSLAEVNETEVKDLTTPDRQHIKD
                     SRISLENTSVEVANMLKKLEMLRMHIYKAERL"
     gene            complement(3187..3564)
                     /locus_tag="PKJ16_02775"
     CDS             complement(3187..3564)
                     /locus_tag="PKJ16_02775"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV45937.1"
                     /translation="MSEKRKYRRYACDIKTTFEFFEGDPDTVDVTITIPSNGAGRIID
                     ISRGGVFIASNDRVSISNIIRVSFALKKEKRTAEGIIVRTGLLRNNPSEAALRFANRK
                     VKEDSYIAVRFTDPLPDFTDKDL"
     gene            3796..5442
                     /locus_tag="PKJ16_02780"
     CDS             3796..5442
                     /locus_tag="PKJ16_02780"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HNV45938.1"
                     /translation="MNTKHTRFIFVTGGVCSSLGKGISTASLGLLLEGHGYRVSLIKM
                     DPYINIDPGTMSPFQHGEVYVTEDGAETDLDLGYYERMTNARLTRKNSVSTGQIYHAV
                     IERERRGDYLGKTVQVIPHITNEIKKRIYDVATDDDLDFVLVEIGGTVGDIESIPFLE
                     AIRQIRHDLGVARVLNVHLTLIPTITVAGEQKTKPTQHSVKELMELGIIPDILLCRTN
                     RPLSADMKSKISLFCNVSERHVISAVDIASSIYEIPRMLHENGYDAIVLEHFGLAPTE
                     LSIPAWDSFMDALHDPQVTVRIAVVGKYISLQDSYRSIYEALGHGAVANRATLEIVRI
                     DSEDIEKKHKLNVKGLFQKVDGILIPGGFGNRGIEGKIATVAYARKNGIPLFGICLGM
                     QCTVIEFARNACGLEGANSSEFDPATEHPVISLLEEQEMIETKGGTMRLGAYQCDLRE
                     GTKARALYGRESVMERHRHRFEFTMRYRELFEENGMQCSGINPKSNLVEVVEVRDHFW
                     LVCTQYHPEFKSKPTAPHPLFVGFIKAAIDSQKGKGAGSE"
     gene            5439..6254
                     /gene="kdsA"
                     /locus_tag="PKJ16_02785"
     CDS             5439..6254
                     /gene="kdsA"
                     /locus_tag="PKJ16_02785"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HNV45939.1"
                     /translation="MKLCGHVVDPAGRFFLIAGPCVIESRDLVLRTAERLTEIAARLG
                     LFFIFKSSYDKANRSSITSFRGPGIDAGLAILDEVRRQFGVPVTTDVHSVEEIGIAAG
                     AVDVIQIPAFLCRQTDMLIAAGRSGAAVNVKKGQFVAPSDVAPIVEKLTASGCESYGI
                     IERGYTFGYNNLVVDMRSFAIMRAMGVPVIFDATHSTQLPGGGTQSGGERDYAPLLAR
                     SAVAAGVDGLFMEVHPCPDEALCDATNQYYLDRVEPLLATLIEIDRLAKGTGG"
     gene            6251..6742
                     /locus_tag="PKJ16_02790"
     CDS             6251..6742
                     /locus_tag="PKJ16_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006073282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HNV45940.1"
                     /translation="MIDWKAVRMLITDVDGVLTDGSLYYDANGESIKRFNVRDGLGIK
                     RLMAAGVKCAVITGRTSQIVEARLRELEYDAVLQGVIDKAAGLSRVRKLLPGISAHEI
                     AYIGDDVNDLPILALVGIPIAVADAADEVLRIARHVTAHVGGNGAVREVCDLILAAKS
                     GSV"
     gene            6810..7373
                     /gene="lptC"
                     /locus_tag="PKJ16_02795"
     CDS             6810..7373
                     /gene="lptC"
                     /locus_tag="PKJ16_02795"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNV45941.1"
                     /translation="MKVFARIGPAVALCVACVACGQGGFLSPARAIPDMRIKNFQSTN
                     YQNGKLLWELAAEESLYFFSENRSVARGIVVSYYQNNKLTATVRADEARIVANSRNIV
                     LSGNVDALMPSGNRLVTSSITWNNHDKVLDTDDPVRIVMSNGDVIEGIGLKANYDLET
                     FELKRRVTAKATETDAIIPNKPHAKKR"
     gene            7357..8649
                     /gene="lptC"
                     /locus_tag="PKJ16_02800"
     CDS             7357..8649
                     /gene="lptC"
                     /locus_tag="PKJ16_02800"
                     /inference="COORDINATES: protein motif:HMM:NF018537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HNV45942.1"
                     /translation="MPRSGNIIVALIAAGALILCWSNDPAAVSAPAKNKKKKNVPVVI
                     SGGSVKGALQGIVGGKKMYQTELGGNPEITWGQMRLSTGRIVVRGAQGDTAECTGGVR
                     ISDGKTKSTIRASRALFTRKDNHIELTGGVEITTRRSDDGSTVLMRSKRVIYRINDEV
                     AEALGGVRVTNADILIVSERAVYRKRENVVSFEIKPIAKKGPNIFRAATLSYELGERR
                     IVLDGNVYIETYTDEKDAKTSAPRRVRSITTGDRAEYLDAAERRVTVLGNARIVRDDA
                     TFAGKRIDMLGKDGDRVLGDDIVITYTAEHVDVKGKRLSYNKANKYASISGDSIIIIR
                     DPKVPGKEKARMYGDYIEYFQDIEELFINGNVSIHQDNGVIRGDVARYLRREDAVYIM
                     GNSRIERAGSTLTANMIYFNTKSNNVRLIGNIYGTGVN"
     gene            8684..9448
                     /gene="lptB"
                     /locus_tag="PKJ16_02805"
     CDS             8684..9448
                     /gene="lptB"
                     /locus_tag="PKJ16_02805"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HNV45943.1"
                     /translation="MQNGDTYGDARKTGRKILELDSLVKIYHKRRVVNRISFEIMSGE
                     IVGLLGPNGAGKTTTFYMVVGLVKPDAGKIILNNTDITNEPMYRRARLGIGYLPQEAS
                     IFRKLTVRENLDIILEMQDLTKQEMEEKRERLLSDLSISHLAPQKAYTLSGGERRRLE
                     IARTLVTDPDFILLDEPFAGVDPIAVADIQSIISALKERGIGVLITDHNVRETLTITD
                     RSCIIHEGKILISGKANQLIRSKKAREIYLGEEFSL"
     gene            9458..10906
                     /gene="rpoN"
                     /locus_tag="PKJ16_02810"
     CDS             9458..10906
                     /gene="rpoN"
                     /locus_tag="PKJ16_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HNV45944.1"
                     /translation="MAQSPRLELRQSQKLFLTQTLRQSIEMLQLSTVELMEMIRAELT
                     ENPVLEETSSEGAATVGSADDREGLLERSLSGDEAPAGEKGEGTIEYERYGDSAYSGH
                     VDDDSNRRFIENTIRYGESLQDVLLRQARIVARDIAEEDLFERIITALDENGFVRLSP
                     GEIAEGAGVPIEAVVHAMGVIQEFDPPGCATTGAREALLVQARAQYPNDALLAEIIER
                     HFDDLDNVRFERIAKALGVEIGAVMDRVEKLHHLDPFPGRQFSRQGARYIVPDVEARL
                     DGGELAVTMKDDWLPDIDINSYYSRLLDEKKIEKKHKEYIQDKLQSARNLIKNIRTRR
                     TTIARVVGAVMERQRAFLDRGPGHLRPLTCREIADDLGYHESTVSRAMSNKFVQTAWG
                     IFEMRSFFVSRVGNEGVEAASSDRVQALIRETIAGEDPSAPLSDEDICERIREQGIHP
                     SRRTVAKYRGELNIPPAHTRKRIHIYKTGEHS"
     gene            10903..11706
                     /gene="raiA"
                     /locus_tag="PKJ16_02815"
     CDS             10903..11706
                     /gene="raiA"
                     /locus_tag="PKJ16_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HNV45945.1"
                     /translation="MNIKITGRNFDVTPSIREYAEKKVKKLETYFHQLLDASIIMFLE
                     RQDHVMEILINGDGVQFYARERSGDMYSSIDLLCDKVEKQIVRHKERHSGHKAVGLGK
                     MEEKEVSASSAVTLTMNRVSNKPLDPIEAFLEMRLEKRDFILFKRGIQDLQSDVDYSS
                     RNYSIIFKDGAGYRMVEVPFEFISGNAVAGQGLIGYDIKILDESAASPRFEYVKNKDV
                     TLPSITPAQAYEKLVADGASHLPFFNADTNFLNIIYRDGKRFSLMVPTF"
     gene            11722..12678
                     /gene="hprK"
                     /locus_tag="PKJ16_02820"
     CDS             11722..12678
                     /gene="hprK"
                     /locus_tag="PKJ16_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HNV45946.1"
                     /translation="MSAKKFRIADLISIKGKLDLGLTLVAGAAGLDKVIKGSEINRPG
                     LTLAGFYEFFAFDRIQIFGLGETAFLHGLDDARKREVYEKLFSYDMLCCIFSHGERPD
                     DIFLDFSEERGIPVLLTGLETTRLITLITHIIEEAYSPSITLHGTLVEVFGIGVLLTG
                     KSGIGKSECALELIERGHRLVADDLVEVKRLDEYLLMGSGSEIIKHHMEIRGIGIINV
                     RDIFGIRSVRNRKRIELVALLEEWQSGKEYDRLGIEEEVYTILDVDSPYITIPVRPGR
                     NIPIIIETAALNRRLKKMGVFSASELDMRIQNWMKQEGAAHD"
     gene            12671..12937
                     /locus_tag="PKJ16_02825"
     CDS             12671..12937
                     /locus_tag="PKJ16_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HNV45947.1"
                     /translation="MTEREVRITSNAGVHARPAMMLVKEAMKYPCEVFVIKDDIEANG
                     KSIMSVLGLAITPGSRLLIKAVGDREREAVERLVRLIESDFKDL"
     gene            12975..13664
                     /locus_tag="PKJ16_02830"
     CDS             12975..13664
                     /locus_tag="PKJ16_02830"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HNV45948.1"
                     /translation="MTREFEGGAREELRAYVRQQPPRGGMVWYRSDEERSYLSAWLAA
                     GDERSPRIVRDTPADSPGVVRDERAACTRCENVVRKRGPFGSGANGIMIILNMPSRIT
                     DFEKKFYAADADELLAKMMGAIGIDLAECYVTELVKCDPTGSASSAGALFDNCRAVLT
                     RELEERAPRFVVVMGELAPLQKIIHRSRGILWYAIEHPVVLLKNPDLKKPAWNTLKVL
                     RTKLAEPSAGR"
     gene            13666..15639
                     /gene="priA"
                     /locus_tag="PKJ16_02835"
     CDS             13666..15639
                     /gene="priA"
                     /locus_tag="PKJ16_02835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HNV45949.1"
                     /translation="MGRAVYADVHVGTPVEGPFTYAVPEGMTVVPGMRVIVDFNRRRM
                     TAFVVDVHARIPDFSPIKPIVECIDPEPVFNERLVDLARFTASHYVAEVGEVMALALP
                     AGTSRGTRSRDNTSATQGGRAIELNEEQRVVRDAILEGTARGENAHLVFGVTGSGKTE
                     LYLELARLTIEAGRSVLVMVPEISLSSQIFRRIHDVFGGDLVLYHSRLTPNQRFDNWM
                     RFFHGEARIAVGTRSAVFMPCPDLGLIVIDEEHDGSYKENSTPRYNARRLAFYRSRTE
                     NALLVMGSATPSVETLYSTERGVMRLHRLTARYGTAEQPEIDIVTVKARDGQDALSSR
                     LRLATRRTIDAGGQAIFLLNRRGFAPVMICDECSQFVSCPQCSISLTLHNDNRLHCHY
                     CGHLQGAPESCPACGAEALVSLGAGTQRAEEAVRAAFPDARLARMDLDSTRRKNAAAD
                     IIAAMEAREIDILLGTQMVAKGFDFHGVTLVGVLLADIGMSLPDYRATERVFSLLVQV
                     AGRCGRGAEAGRVLVQTLNEEHRLFSFLRRHDYLGFYRYELSMRRELGYPPFTRIARL
                     LVRGKDESQVEAAVQSLARELAGIIKRRQAAVQLLGPSMAPLYRIAGNYRSHILLKSR
                     EVDPLRAVVAEGKRAFASRGVYLEIDIDPYDML"
     gene            complement(15652..16869)
                     /locus_tag="PKJ16_02840"
     CDS             complement(15652..16869)
                     /locus_tag="PKJ16_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNV45950.1"
                     /translation="MKKFIYVMLALSIAGVALSAYLTVEHYYPEIGSALRLCGNDLNS
                     ACASLRNSPYSSVFGVPIAGIGLLFYLFILFTVLVADYAGERYYTYAAIIAFPATALA
                     LAVDIALAIILVVTGLFCGFCIATYGVTALMFATSILWYRFALRDAPGGLRGTVRQLV
                     NADESGPDRRAAVSLYVIFGFLLLFTVFSFNYILFLKTFTPAAPRAAVDEHLRTFYAA
                     APERLELPPSALTIGPANARVRIIAFTDFLCSACSHLFEVEKQLFAQFKGSLAVTYYH
                     YPLDTDCNPFAKRTTYPHSCSAARAMSAAAQKGFFPRYLSLHFKYYRKISGGYTADTA
                     RALADSIAPDPGFMSLMGSPAVSAIIERDARLAEKLGISATPTLFINGRRMVGVPPVE
                     LLSAIIRREMGGQ"
     gene            complement(16901..18199)
                     /locus_tag="PKJ16_02845"
     CDS             complement(16901..18199)
                     /locus_tag="PKJ16_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV45951.1"
                     /translation="MTPRRRNRPQLVYCDERDRIYEHPSLEPAFRSGTRFVPVDPAEL
                     IELPFGSHILSLPGRRPVARRKRTDAFECIPDDDAGVPIVAAASFLSSGFLRTFLPGY
                     TVTADAPTLPLWAYAGVAIVDGEYAVPAMRIDEDPRSDPELHLDDAALTRAQRSLAQR
                     LPDNRLVRQLAHCSTEYRCLCARNFFLGRFEAPVPTTPACNAACVGCLSLQEDSGFCH
                     SQDRLDFSPTPEEIAEVILHHFSRVPDGVASFGQGCEGEPLLRARDLSRAIELVRAHT
                     ARGTINLNTNGSLPDRIPDLRAAGLDSIRISLNSPTERYYNRYVRSSSFSFSDMRRSV
                     ETALGCGLFVSLNLFFLPGFTDMESEVDSLFAFLRDHPVHMIQTRNLNIDPDLYWAAI
                     GVEETNAIGIRTLCATLRERHPAIRLGYYNPPREKFGFSN"
     gene            complement(18180..18446)
                     /locus_tag="PKJ16_02850"
     CDS             complement(18180..18446)
                     /locus_tag="PKJ16_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45952.1"
                     /translation="MEESSRTSTALHNELRDLRASWRQVRMERLGIKRTLEGSGIDAR
                     TIRRSPAYRRLVKEQHRLSTRIRHIEKKLHRANAIRIGHDTTKT"
     gene            complement(18497..18694)
                     /gene="thiS"
                     /locus_tag="PKJ16_02855"
     CDS             complement(18497..18694)
                     /gene="thiS"
                     /locus_tag="PKJ16_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HNV45953.1"
                     /translation="MKIKVNGIAIEFYKTSLLDLVVQYRFNPDKIVVERNGAIVSPES
                     YPAIHLQDGDLIDIARSTGKK"
     gene            complement(18699..19433)
                     /locus_tag="PKJ16_02860"
     CDS             complement(18699..19433)
                     /locus_tag="PKJ16_02860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HNV45954.1"
                     /translation="MPRFYIPSPTIVDGRVRIDGDLFHHAVRVRRAGPGEIVEYTTAH
                     GDAYRAAIVEISADNALLEIIDKIDTRFSSVNIVVAVALLKGKKFDLVVQKAVEAGVA
                     GIQPMITERSIPRLEDDGEGRLNRWRRIAAEAAQQCQRGTPPPVFAIRDFHEVIAGFA
                     ESVRMIAHLDPRARDLKEFLRSRPMLGDDGRAGTVLIGPEGGFSERECAAARDAGWEA
                     VILGGTQMRAETAAIVLPAILIYEWS"
     gene            19538..20995
                     /locus_tag="PKJ16_02865"
     CDS             19538..20995
                     /locus_tag="PKJ16_02865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV45955.1"
                     /translation="MKKYLVLAAAVFVLFTAVKLHFISSTMVIDDEAYYGMYSRHLAP
                     GYIDHGPVVALLVRASTAAFGETAFGVRAGSVALLSLLSIALFWFGARFHSPRAGWAL
                     FLISMTTLLYFAGSVVVTPDTPMVFFMMLALMLYYAAFFSHRYYFYPAGIMLGLAMLS
                     KISVVFPALGVFLFPLIAPDKRAFLRCREFYLSFIIAAVVFSPFLVWNFQNDLAFIRY
                     QGAHVFRPGGIKSFFDLWGAQAGLLFPILFFFAIYLPGQAIVNRIRARAVDPRRLFFA
                     ITAAVPLGYFMVQSMFSRYEANWPAPAFFGGIMLAGMHIAEHWERLRTLFAVQVAASC
                     ALIAVAVVQVVYSPIAFPAGKDVTNRYYAYQALAGPVREFLEREPRYRSLRMMSANYQ
                     IPSMVNLYLRPDREAVAISVAGYHDTLYSFVYRDATLVGQDYLFVWLGEEIPNNIRRH
                     FNSLERVATFPSVRAGRVVGVVTLWEAKGYRGRAF"
     gene            complement(20992..21795)
                     /locus_tag="PKJ16_02870"
     CDS             complement(20992..21795)
                     /locus_tag="PKJ16_02870"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HNV45956.1"
                     /translation="MAKSLPILIIVPHGGYRVPDELKGYERVNRFDLFYSADSCANDL
                     FAFGDRGATVISTDISRLFVDVDRPHTLLPPGSDDGVIKRETPAGKPVFDEGAFPDEI
                     AIANLIRRYHLPFHTQVEAATQSVGHALIIDCHVMMPVGPKSAADAGRPRPLVTIENA
                     AHGRSGRYTTCPDELAGALKQALEKFFESEPASVAGRIAINTPSFNGHILNRYGNAGI
                     PMLRLSVSRSLFFTERYFNETTMRVDAARISALRERLWGGIERFFGKYF"
     gene            21952..22458
                     /locus_tag="PKJ16_02875"
     CDS             21952..22458
                     /locus_tag="PKJ16_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019638430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HNV45957.1"
                     /translation="MKPYKIDQLSVNILEILNDNAKTSHADIARQLAVKEAEVAKAIK
                     RLEKDRIIVRYKSQINWERIKHDEVRALIEVKVTPMRGVGFDAIAEDIYRFPEVNSVY
                     LLSGSYDLLIQVEGPNLREVSLFVSEKLSTIKNIQSTVTHFILKKYKEDGVVMVDRSE
                     SKRLPVSL"
     gene            22455..23144
                     /locus_tag="PKJ16_02880"
     CDS             22455..23144
                     /locus_tag="PKJ16_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018231606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HNV45958.1"
                     /translation="MNAPLSLNRVFRALLRAYGPQHWWPGDTPFEIAIGAILTQNVAW
                     TNVETAIAQLKEKRLLAPRALDAIPAPRIAPLIRSTGYYNQKAIKLKNFLAWFAGYRY
                     SFARLHGVDTERLRRELLAVSGIGPETADSILLYALGRKLFVVDAYTVRLLGRLGIIG
                     GGESYHDVQGLFHRRFRGGVGEYNEFHALIVNHGKYVCRTRPRCAECCLSRRCLAYHQ
                     QGADAAHAVAG"
     gene            complement(23094..23903)
                     /locus_tag="PKJ16_02885"
     CDS             complement(23094..23903)
                     /locus_tag="PKJ16_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45959.1"
                     /translation="MIPLIPRLKTLACVLPILFTAFTPPTGDRGDTGVPTEYYYALQS
                     LKQAAPPRVVDMIKTDNARLGKRVIRRGTLVTYSNRKAASVRIAGDFNAWKPVPMTRS
                     ASGVWYHFLPFGNRAVGDSAGNESEHAPSSAPPIRYKFTVDGIWIADPLNPDRIDDGA
                     GSFVSRIAPGTRPENRHDSYRIIGRGTVEFRCYHPNASYVAVVGDFNAWNPENDVLKK
                     TSAGFWRLTKRLSPGTYRYQYIIDGVWRPDIYNAKSSGDGMGGISSLLVVR"
     gene            24154..27456
                     /locus_tag="PKJ16_02890"
     CDS             24154..27456
                     /locus_tag="PKJ16_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45960.1"
                     /translation="MGDKEERISYTADEQKEIDRILSFVDTETDRLMSGFTKTATLER
                     ESLPEEFQDDDREAGAYREMEDDVADIDRDMPEDEDIVDITDMVQEVEEPAEPSGAGE
                     FDDLDSLVRDDERRPRSPEARDEADELDTLSTADLDEVADEKPAFKYKPKTKPAEGDE
                     EPSTLDELEALAAGEAVSGDEARENEAVPADGDELASFDDFDMPAEKPREAGKGVSIG
                     GDAESDIPDLSDISFDEAGEMPEAKDEDIPEIDLGGLDDFSAAEKTVPEKSRTAADEA
                     PMSFDEELPSFDDDALAAPSRAESEIAPRAAEKRAVADDDGFGDIPSIDEIDDIVAKE
                     EPAIAAGAYDDDFGMAEPSQTKAASFTEPARERESAPPKEKPAKGARREEQRDGLDLS
                     DRDLKKLKNGLTLLHPNLRKAVRDIILNDVLPEQDARQVLDAIITGKSEDAVLKIVQN
                     KLGKTIDISEPAAGAAGRRVIAGRPEYTKDGIERQKKLLFLTKIFGAAAAVTAVLVVV
                     LYQFWYKPYMAERYIAKGVALIREPGDPVTKKVKDYTKAEELFTFVDENYKKDYLRGY
                     NEYARAYFDKREFDLSLRKLNAAYRIDPVHPETLNSLGYFFSRIPEGYFKQIRPRLKD
                     WYYKEVPPVGSAETQLDVAVDFYRRVLNREPKNITALFGIGNAYKYQGQYLKARQYYE
                     NILKIDRKSVVGYSGLLDLFIDRDNFPEVLTVHSDLQDKDLMVKLPSPLLAKLASYYL
                     GKKRTDRMNIRVDYGVESPRLKDIADNPYPVVRSVLSALAERDADYPPLYVHLALLAR
                     AQDNPKLMMENLKRAIKRAEARDQQYFAAYHLMGEYHLHVKEPVQAYQNFNRAIKAYS
                     SPADFMREDFYAETESLGKTYAMIGNVFYYFFDKVRHRFGDEIEDRELDQDVEKFANY
                     RIARDKYELAVQEGYKSSELFYNLGRIYYMKGLFENYEKALESWLNLYEDFSARPELM
                     YAVGNAFYHLDNLEASKGEFLKLISVFEHQAERLTAVHPEKAAHVRIFQTLASAYNNL
                     GAIYQRQNNESKSTICYWKSIDYAARLRRESEFARVNLARSFKARQEEISPIIDENVP
                     YAIDIYSEDMRK"
     gene            27540..28130
                     /locus_tag="PKJ16_02895"
     CDS             27540..28130
                     /locus_tag="PKJ16_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45961.1"
                     /translation="MQRLPILYLYKKVVFPSCALTTGTREKRHRGLAAGDRVLALPIR
                     GFFDILLARRGVATLSEVTSVEVAEDTVTVGLKGISRVRLRRIDRFLTAEHVPIPENG
                     AWARDGIVNELRKKSQELIFLINVKESDKLIGLLGFINNPSQLSDFVANYFIVSFPRR
                     YRIHREIDGARRLRNLMAVLDELIAKFREKREAGVS"
     gene            28127..>28332
                     /locus_tag="PKJ16_02900"
     CDS             28127..>28332
                     /locus_tag="PKJ16_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45962.1"
                     /translation="MKKKTDESGAYYTIMWSKMYKYDKYEAQRILPELTGILCLLEKK
                     DSRDPRPILFYNCWREGLKIAIKNL"
BASE COUNT         5451 a         8593 c         9114 g         5174 t
ORIGIN      
        1 tcccatcatc ggctggcgct cgaatatcga gtttctgcag ctcgaccgga tgcgcgagtt
       61 tttcacgcgg cactatgtcc ctgcgcgcat gagcatcgcg attgtcggga agcagaaccc
      121 ggcggagacc ctcgccgtgc tcgagcgctc attcggccgc ttacccaagc gtccggagcc
      181 catgcccgcc gccatacgcg agcccgcgca aaacggcgag cgccgtttcg aggtcttttt
      241 cgacgccaat ccgtccctcg tgatggggtg gcacaagccc acgcttccgg ccaaggccga
      301 ttttgcctgc gaggtgatcg cctccctgct ctccgacggg cgctcctcgc gcctgtatcg
      361 ccggctggtc ctggagcgaa aaatcgcctc gagcgtgagc gcctggaacg gctttcccgg
      421 gtcgcgctac gacaatctct tcgtcgtgat ggccgcgccg cagggatcgc acaccctgcc
      481 ggagatcgag gcggcggtgc tcgatgagat cgcgcggctc gccggcgacc tccgcgacga
      541 cgagctcgag cgcatcctca atcgcatcga gaccaaggcg atcctgggcc tcgactcgaa
      601 catggggatc gccagtctca tcagccacta tcagaccatc atgaaggact ggcgtttcgc
      661 cacgagctat ctcgacgaca tgcggcgcat cacccgcgcg gacgtcgtcg acgcgatcaa
      721 cacctatctg gtggcgaaga accggacggt gggctacctg ctgcgaacgc gcgaccagaa
      781 gaagtgatgc gcggcgccct ggatattcac gggacaagga caatgaacat gatcattcgc
      841 ggaaaacgat tctatgggcg tattctcgcc gtcgcaacgg cgtgtgtaat gatgatgatt
      901 cccctcgcaa cggggaccgc ccagcaggcg accatggagc tgccgcgcgt ccagtcgtgg
      961 aaaacgacca acggcatacg cgtggtctac cttgaggacc ggcttccgca gctgacggtc
     1021 gtcgcgacga tcggctttgg gacgctctac gagaacgagc gcaacgccgg gattgcggac
     1081 ctgcttgcgc gcaccctgaa cctggccgga tcgaagcggt atcccggtac ggcgatgcac
     1141 gaggcgatcg aatcggttgg cgggaccatt ggggtttcat cgagctggga agggatcacc
     1201 gtcaccgtgc gggtgctcga acggcatgcc gcgcgcgcat tcgatgtgct gggggacctt
     1261 gtcaccaacc ccaactggga cgcgcgcaag ctcgaggacg cccgggcgct cctcatcgag
     1321 accatcaagc ggaaacagga tcgtcccttc gagctgggct acgaggcgct gcgcgaggtt
     1381 gtcttcgccg gcaagggcta tggggcgatc gcgcgcgaaa aaacggtgcg cgcctgcacg
     1441 ctcgacgatc tcacgtcggt atggcgcacc tatgccgtgg cgggaaacac gctgctgggc
     1501 gtgtccacgt ccatccccgc gcccgcggtg cggcgtcacg cggaggtcct gcggggggtg
     1561 cctgcgggcg acgcaaccga ttacgcaatc gacgcggccg ccgttcgcgc gtccatcgcc
     1621 accaatgccg gcgtgatcta tctggttccc aaggacattc cccaggccac catactgatc
     1681 gggacggtcg cgccggatat cgcgagcccg aacacgtccg cgctcagggt gatgaactac
     1741 attctcggcg agggctcgtt caactcccgg ctcatggagg aggtgcgcgt gaagcggggg
     1801 ctcgcctatt cggtgcagtc cgtcatcggc ttccggagaa acgcggggat tttcttcgcc
     1861 atggcccaga ccaatgcgga tcaggcgacg gtcaccctcg aggtgatgcg cgaaaacatc
     1921 gcgcgcatgt cccgcgagcc cgtgagcggc gaggagctcg tctgggcgcg ggagtcaatc
     1981 gccaacagct tcgtgttcga gttcgatacc tcgctcgcgg tggtgcagaa gtacctctat
     2041 ttgcgctata accgcctgcc ggactcctat ctgaccgact acatcccgcg catacgcgcg
     2101 atctctgcaa cgcgtatcca ggacgaagcg cgttccctgt tcggcggcgg gacggtaacg
     2161 gtggttgtcg ggcgtcggga tatggcgccc gcccttggga agttcggccg ggtggtggtg
     2221 ctgggggacc gctaagccta cagacgctcg gccttgtaga tgtgcatgcg aagcatttcg
     2281 agctttttca gcatattcgc cacctccacc gaggtgttct cgagtgaaat gcgggagtcc
     2341 ttgatgtgct ggcggtcggg cgtggtgagg tccttgacct cggtctcgtt cacctcggcg
     2401 agcgacacga gcgtctgatt gacgatcgcg gccagcgact tgtacatcga gaccagcgcc
     2461 cagtacatct cgtacaggaa gatgaaccgc atctgctcgt cctgcgatcc gaacgcctca
     2521 acgatgtcct tggtctgggt gtcgagtcgg gcgatatagt attgcaccga gcgggggaag
     2581 ctcaccagct ccttgttgag tcccatcacc ttgtcgatct tctggatccc gtcgaggtcc
     2641 aggttccgaa gcgcggtctc gatcgacgtt ccgatctctc gggagacctt gctgctgatc
     2701 tgcgtattgg agatgttgat catcatcccg tggaggcgcc ccttgtgcgt tttgagcagc
     2761 tccagcgcct tgtttaccac atcgtccggg tcgaacccgt cgagcacgta gctgaccgcg
     2821 gcgtcggcgc catcggcgtc cccgatggcc gtctcgtcga tcggggcatc cgcgcgctcg
     2881 aaaatgtccg agatggtgtc cggcgaggcc ggcgtcgatt cggactcagg gcgggacgac
     2941 ttgccggaac gcttgctctc cacgaccttt tgttgataga atccggccga gttcgccggg
     3001 gttcggatga tgcggatgat ctcgattttc ctgtcgcgcc ggttgtagcg caggccgtac
     3061 atgcgcccct cgggatcgat gtagcggttg ttgaggtcgt acacggtgat cttcgagggg
     3121 tctatctcgg agatcgcgcg aatcttgatg aacttggttt tcacgacgga tcgatggtcc
     3181 cgcggcctac aggtccttgt cggtgaaatc cggcaggggg tccgtgaagc gcacggcgat
     3241 gtatgagtct tccttcacct tgcggttcgc gaagcgaagg gccgcctcgg agggattgtt
     3301 ccggagcagg cccgtacgga cgatgatccc ctcggcggtg cgtttttcct ttttaagcgc
     3361 gaaggacacc cggatgatat tgctgatgct cacgcgatcg ttcgaggcga tgaacacgcc
     3421 gccgcggctg atgtcgatga tgcgcccggc gccgttggag ggtatggtga tggtgacgtc
     3481 gaccgtgtcg ggatcgccct cgaagaattc gaaggtcgtc ttgatgtcgc acgcgtatcg
     3541 tcgatatttt cgcttttcgc tcattggcgc ccgccgtcgt atcgtgtccg ggccgcggtg
     3601 cgggacattt ttgtcccccg cggaaccgtg tgaacattgg tagcgtttca aaaaggtatt
     3661 tgtcaagcca tgttttatga aatttccaaa atcatcattg acggaatcat cttcgccgtg
     3721 aagcctgtga ctcggcgatg ggcgggcacc cgcaccgtat cgccacagac gacctgaggg
     3781 cggcgccatc aacctatgaa cacgaaacac acccgtttta tttttgtgac cggcggcgta
     3841 tgctcgtcgc tggggaaggg gatctcgacc gcctcgctcg ggctgctcct cgaggggcac
     3901 ggttaccgcg tctcgctcat caagatggac ccgtacatca atatcgatcc ggggacgatg
     3961 agccccttcc agcacggcga ggtgtacgtg accgaggacg gcgcggagac ggacctcgat
     4021 ctgggctact acgagcgcat gaccaacgcc cgcctcacgc ggaagaactc ggtctccacg
     4081 gggcagatct atcatgcggt catcgagcgg gagcgacgcg gcgactatct ggggaagacg
     4141 gtccaggtga tcccgcatat caccaacgag atcaagaagc gcatctacga tgtggccacc
     4201 gacgacgatc tcgatttcgt gctcgtggag atcggcggga ccgtgggcga catcgagtcg
     4261 atccccttcc tggaggccat ccgtcagatc cgccacgacc tcggcgtcgc gcgcgtgctc
     4321 aacgtgcatc ttacgctcat ccccaccatc accgtggccg gcgagcagaa gaccaagccc
     4381 acgcagcact cggtaaagga gctcatggag ctggggatca tcccggatat actgctgtgc
     4441 cggacgaacc ggccgctctc cgcggacatg aaaagcaaga tttcgctctt ctgcaacgtg
     4501 tccgagcgcc acgtgatctc ggcggtcgac atcgcgagct ccatctacga gattccgcgc
     4561 atgctccatg agaacggcta cgacgcgatc gtgctggagc atttcggcct cgcgcccacc
     4621 gagctttcga tccccgcctg ggactccttc atggacgcgc tgcacgatcc gcaggtcacg
     4681 gtgcggatcg cggtggtcgg gaaatacatc tcgctgcagg actcgtaccg ctccatctac
     4741 gaggcgctgg ggcacggcgc ggtggcgaac cgcgccacgc tcgaaatcgt gcgcatcgac
     4801 tccgaggaca tcgagaagaa gcacaagctc aacgtgaagg gcctcttcca gaaggtggac
     4861 ggcatcctga tccccggcgg gttcgggaac cgcggtatcg aggggaagat cgccacggtc
     4921 gcctatgcgc gcaagaacgg gataccgctt ttcggcatct gcctgggaat gcagtgcacg
     4981 gtgatagagt tcgcccgcaa tgcctgcggg ctcgaggggg cgaattcctc cgagttcgat
     5041 cccgcgaccg agcaccccgt catctcgctg ctcgaggagc aggagatgat cgagaccaag
     5101 ggcggcacca tgcggctcgg cgcctatcag tgcgatctgc gcgagggcac gaaggcccgc
     5161 gcgctctacg gccgggagag cgtcatggag cgccacaggc accgcttcga attcaccatg
     5221 cgctatcgcg agctcttcga ggagaacggc atgcagtgca gcgggatcaa tccgaaaagc
     5281 aatttggtcg aggtggtcga ggtccgcgat catttctggc tggtgtgtac gcaataccac
     5341 cccgagttca agtccaagcc aaccgctccg catccgctct ttgtcggatt catcaaggcc
     5401 gcgatcgact cacagaaagg gaagggggcg ggatccgaat gaaactgtgc gggcatgtcg
     5461 ttgatccggc ggggcggttc ttcctcatcg cgggcccctg cgtgatcgag tcgcgggacc
     5521 tggtgttgcg cacggccgag cgcctcaccg agatcgccgc gcggctgggc ctgttcttca
     5581 tcttcaaatc atcgtacgat aaggcgaatc ggagctccat cacctccttc cgcgggccgg
     5641 ggatcgacgc gggcctcgcg atcctggacg aggtccgccg ccagttcggc gttcccgtga
     5701 ccaccgacgt gcattcggtg gaggagatcg gaatcgccgc aggcgcggtt gacgtgatcc
     5761 agatcccggc gtttttgtgc cggcagaccg acatgctcat cgcggccgga cgcagcggcg
     5821 ccgcggtgaa cgtgaagaag gggcagttcg tcgccccgtc cgacgtcgcg cccatcgtcg
     5881 agaagctcac cgcgtcgggg tgcgaaagct acgggatcat cgagcgcggg tacaccttcg
     5941 gttacaacaa cctggtggtc gacatgcggt cgttcgccat catgcgcgcg atgggggtgc
     6001 ccgtcatctt cgacgcgacg cactccacgc agctccccgg cggcgggacg cagtcgggcg
     6061 gcgagcggga ctacgcgccg cttctggcgc gaagcgcggt cgcggcgggc gtggacggcc
     6121 tgttcatgga ggtccatccc tgtcccgacg aggcgctctg cgacgcgacc aatcaatact
     6181 atctggaccg cgtggagccg ctccttgcga cgctcatcga gatcgaccgg ttggcgaagg
     6241 ggaccggcgg atgatcgact ggaaggccgt gcgcatgctc atcaccgacg tcgacggcgt
     6301 gctcaccgac gggagcctct attacgatgc gaacggcgag tcgatcaagc gcttcaatgt
     6361 ccgggacggg ctcgggatca agcgcctgat ggccgccggt gtgaagtgcg ccgtgatcac
     6421 cggccggacc tcgcagatcg tcgaagcgcg cctgcgcgag ctggaatacg atgcggtgct
     6481 gcagggcgtc atcgacaagg ccgctggtct ttcgcgggtg cggaaactcc tccccgggat
     6541 atccgcgcac gagatcgcct atatcggcga tgacgtgaac gacctgccga tactggcgct
     6601 ggtcggcata ccgatcgccg ttgccgacgc cgccgacgag gtgctgcgca tcgcccgtca
     6661 cgtgaccgcg catgtaggcg ggaacggggc cgtgcgcgag gtgtgcgacc tgatcctcgc
     6721 cgcgaaaagc gggtccgtat gagctttttt tgttgaaatc ctcccgagtt cgcattaccc
     6781 ttaccataac gattgtgatt ggagtatcgg tgaaagtatt tgcacgaatc ggtcccgctg
     6841 tggccctgtg tgttgcgtgt gtcgcgtgcg gtcagggcgg gttcctgtcg ccggcgcgcg
     6901 ccatccccga catgcgcatc aagaacttcc agtcgacgaa ctatcagaac gggaaactgc
     6961 tgtgggagct tgcggccgag gagtcgctct attttttttc ggagaaccgc tccgtggcgc
     7021 gggggatcgt ggtcagctat tatcagaaca acaagctcac ggcgaccgta cgcgcggatg
     7081 aggcgcgcat cgtggcgaat tcccgcaata tcgtgctctc gggaaacgtg gacgccctga
     7141 tgccgtcggg aaaccggctc gtcacctcgt cgatcacctg gaacaatcac gacaaggtgc
     7201 tggacacgga cgatcccgtc cgcatcgtca tgagcaatgg cgatgtcatc gaggggatcg
     7261 gcctcaaggc gaattacgat ttggaaacat tcgaactgaa gaggcgggtg acggcgaaag
     7321 cgaccgagac ggacgccatc atcccgaaca agccccatgc caagaagcgg taatatcatc
     7381 gtcgcactca tcgcggcggg cgctctgatc ctctgctggt ccaacgaccc tgcggccgtt
     7441 tcggcccccg cgaaaaacaa gaaaaagaag aacgttcccg tcgtcatttc gggcggttcc
     7501 gtgaagggcg cgctccaggg catcgtgggc gggaagaaga tgtatcaaac cgagctcggc
     7561 ggaaatccgg agatcacctg ggggcagatg cgcctgagca cgggcaggat cgtggtccgc
     7621 ggcgcccagg gcgacacggc ggagtgcacg ggcggcgtcc gcatctccga cggcaaaacg
     7681 aaatcgacca ttcgcgccag ccgcgcgctg ttcacgcgga aggacaacca catcgaactg
     7741 accggcgggg tcgagataac cacgcgccgc tccgacgacg gctcgacggt gctgatgcga
     7801 tcgaagcgcg tcatctatcg catcaacgat gaggtcgccg aggccctggg cggcgtgcgc
     7861 gtgacgaacg ccgacatcct gatcgtgtcg gagcgcgccg tgtatcggaa acgggagaac
     7921 gtggtctcgt tcgagatcaa gccgatcgcg aagaagggcc cgaacatctt tcgggccgcc
     7981 acgctctcct atgagctcgg cgaacggcgc atcgtgcttg atggaaacgt ctatatcgag
     8041 acctacaccg acgagaagga cgcgaagacc agtgcgccga ggcgcgtccg gtcgatcacc
     8101 acgggcgacc gcgcggagta tctggacgcg gccgaacgcc gggtcacggt gctggggaac
     8161 gcgcgcatcg tgcgcgacga cgcgaccttc gccgggaagc ggatcgacat gctggggaaa
     8221 gacggcgacc gggtgctcgg cgacgatatc gtgatcacct atacggcgga gcatgtcgat
     8281 gtgaagggga agcggttatc gtacaacaag gcgaacaagt acgcgtcgat atcgggcgat
     8341 tcgatcatca tcatacgcga tccgaaggtc ccgggcaagg agaaggcgcg gatgtacggc
     8401 gattacatcg agtacttcca ggacatcgag gagctgttca tcaacgggaa cgtctcgatc
     8461 catcaggaca acggggtgat tcggggcgac gtcgcgcgct accttcggcg cgaggacgcg
     8521 gtgtacatca tggggaattc gcggatcgag cgcgcgggat cgaccctcac ggcgaacatg
     8581 atctatttta atacgaagag caacaatgtc cgattgatcg gaaacatcta cggcacgggc
     8641 gtcaactgag acgcccggcg gcgggtacgc ggggataggc gacatgcaaa acggcgacac
     8701 atacggggac gcgcggaaga cgggccgaaa gattctcgag ctcgactcgc tggtgaagat
     8761 ctaccacaag cggcgcgtgg tcaaccggat ctcgttcgag atcatgtcgg gtgagatcgt
     8821 ggggctgttg ggaccaaacg gcgccgggaa gacaacgacc ttctacatgg tcgtgggctt
     8881 ggtgaagccc gatgcgggca agatcatcct gaacaacacc gacattacca acgagccgat
     8941 gtaccgacgc gcgcgcctgg gcatcggcta ccttccgcag gaggcgtcca tttttcgcaa
     9001 gctcaccgtt cgcgagaacc tcgacatcat cctggagatg caggacctca ccaaacagga
     9061 gatggaggag aagcgggagc ggctcctttc ggacctgtcg atatcgcacc tcgcgcccca
     9121 gaaggcctat acactttccg gcggcgagcg gcgccgtctc gagatcgcgc gcaccctggt
     9181 gaccgacccg gacttcatcc tgctcgacga gccgttcgcc ggggtggacc ccatcgccgt
     9241 ggccgacatc cagagcatca tcagcgcgct gaaggagcgg gggatcggcg tgctcatcac
     9301 cgaccacaat gtgcgcgaga ccctcaccat cacggaccgc tcgtgcatca ttcatgaggg
     9361 gaagatcctc atcagcggca aggcgaacca gctcattcgc agcaagaagg cccgggagat
     9421 atacctcggg gaggagttct ccctctaggg aggcgcgatg gcacagtcac ccagactcga
     9481 gcttcggcaa tcgcagaaac tctttctcac gcagacgctg cggcagtcca tcgagatgct
     9541 gcagctgtcg accgtggagc ttatggagat gattcgcgcc gaactgacgg agaacccggt
     9601 gctggaggaa acctcctccg agggcgcggc gaccgtcggg agcgccgacg accgggaggg
     9661 acttctcgag cgcagcctga gcggcgacga ggccccggcg ggcgagaagg gtgagggcac
     9721 gatcgaatac gagcgctacg gcgacagcgc atattcgggc catgtggacg acgacagcaa
     9781 ccggcggttc atcgagaaca ccatccggta cggcgagtcc ctgcaggacg tgctcctgcg
     9841 gcaggcgcgc atcgttgcgc gtgatatcgc ggaggaggac ctcttcgagc ggatcatcac
     9901 cgcgcttgac gaaaacgggt ttgtgcgact gtcccccgga gagatcgccg agggcgccgg
     9961 cgtgccgatc gaggccgtcg ttcacgcgat gggcgtgatt caggagttcg atccgcccgg
    10021 gtgcgcgacg accggcgccc gcgaggccct gctggtgcag gcgcgcgcgc agtacccgaa
    10081 cgacgcgctc cttgccgaga tcatcgagcg gcatttcgac gatctggaca atgtccggtt
    10141 cgagcgcatc gccaaggcgc tgggcgttga gatcggggcg gtgatggatc gcgtcgagaa
    10201 gctccatcac ctcgacccgt tccccgggcg tcaattctcc cggcagggcg cccgttatat
    10261 cgtcccggac gtcgaggcgc gcctcgacgg cggagagctc gccgttacga tgaaggacga
    10321 ctggctgccc gatatcgaca ttaattccta ttattcgcgc ctgctcgacg aaaaaaaaat
    10381 tgaaaaaaaa cacaaagaat atattcagga caaactgcag tcggcgcgga acctcatcaa
    10441 gaacatcagg acccgccgca caacgattgc gcgggttgtg ggagccgtca tggagcgcca
    10501 gcgggccttt ctggatcggg gtccggggca cttgcggccg ctcacgtgcc gcgaaatcgc
    10561 ggacgatctc ggctatcatg agtcgaccgt cagcagggcc atgagcaaca agttcgtgca
    10621 aaccgcctgg ggaatcttcg agatgaggag ctttttcgtc tcccgcgtgg gaaacgaggg
    10681 tgtcgaagcg gcgtcctcgg accgggtgca ggcgctcatc agggagacga tcgcgggtga
    10741 ggacccgagc gccccgttga gcgacgagga tatctgcgag cggattcgcg agcagggaat
    10801 tcacccgtcg cgaaggaccg tggcgaaata ccgcggggag ctcaatatcc ccccggcgca
    10861 tacgcgtaag cgaatacata tctacaaaac aggagaacat tcatgaatat aaaaatcacc
    10921 ggcagaaatt tcgacgtcac accgagcatt cgcgaatacg ccgaaaagaa ggtgaaaaag
    10981 ctcgaaacct attttcacca gctgctcgac gcgagcatca tcatgttcct ggagcggcag
    11041 gaccatgtca tggaaatact catcaacggg gacggcgtgc aattctacgc ccgcgagcgg
    11101 tccggcgaca tgtactcgtc gatcgacctg ctctgcgaca aggtcgagaa gcagatcgtg
    11161 cgccacaagg aacgccactc gggccacaag gcggtaggcc tcgggaaaat ggaagagaaa
    11221 gaggtctctg cgtcctcggc cgtgacgctc accatgaacc gggtgagcaa taagccgctc
    11281 gatcccatcg aggcctttct ggagatgcgc ctcgagaaac gggacttcat cctgttcaag
    11341 cggggcatcc aggatctgca gagcgatgtc gattattcaa gccgcaatta ttcgatcatc
    11401 ttcaaggacg gtgcgggata ccgcatggtc gaggttccct tcgaattcat cagcggcaac
    11461 gccgtcgccg gccaggggct catcggctac gacatcaaga tccttgatga atccgccgca
    11521 agcccgcgct tcgagtacgt gaagaataaa gatgttacgc tcccgtcgat cacgccggcg
    11581 caggcctacg agaagctggt ggccgacggc gcgtcccatc ttccgttctt caatgccgat
    11641 acgaatttcc tgaacatcat ctaccgcgat ggaaagcgct tctccctcat ggtgcccacg
    11701 ttctaggggc tgtgcccgcc gatgagcgca aaaaagttcc gtatcgccga cctcatctca
    11761 atcaagggca agctcgacct cgggcttacc ctggttgccg gggccgcggg gctggacaag
    11821 gtcatcaagg ggagcgagat caaccgtccc ggcctcacgc tcgccggctt ttatgagttt
    11881 ttcgcatttg atcgcatcca gatcttcggt ctgggcgaga cggccttttt acacggcctg
    11941 gacgacgcgc gcaagcgcga ggtgtacgag aaactgttct cgtacgacat gctgtgctgt
    12001 attttcagcc acggcgagcg gcccgacgat atcttcctgg acttctccga ggagcgcggc
    12061 ataccggtgc tgctcaccgg tctcgagacc acgcgcctca tcacgcttat cacgcacatc
    12121 atcgaggagg cgtactcccc gtcgatcacg ctccacggga cgctggtcga ggtgttcggc
    12181 atcggcgtac tgctcacggg caagagcggc atcggcaaga gcgagtgcgc gctggagctc
    12241 atcgagcgcg ggcaccggct ggtggccgac gatctcgtcg aggtgaagcg cctggacgag
    12301 tatctgctga tgggaagcgg ctccgagatc atcaagcacc acatggagat ccggggcatc
    12361 ggcatcatca acgtccgcga tattttcggg atccgttcgg tgcgcaaccg caagcggatc
    12421 gagctggtgg cgctcctcga ggaatggcag agtggcaagg agtacgatcg cctgggcatc
    12481 gaggaggagg tgtatacgat cctcgatgtc gacagtccct acattaccat accggtgcgt
    12541 ccggggcgaa acatccccat catcatcgag accgcggcgc tcaaccggcg cctcaagaag
    12601 atgggcgtct tctcggccag cgagctcgac atgcgcatcc agaactggat gaagcaggag
    12661 ggggccgcgc atgactgagc gcgaggtgcg catcaccagc aatgcgggcg tgcacgcccg
    12721 gcccgccatg atgctggtca aggaggcgat gaagtacccc tgcgaggtgt tcgtcatcaa
    12781 ggacgacatc gaggcgaacg ggaagtccat catgtccgtg ctgggtctcg cgatcacgcc
    12841 cggcagccgt ttgctcatca aggccgtagg ggaccgggag cgcgaggcgg tggagcgact
    12901 cgtccggctc atcgaaagcg atttcaagga cctgtgatgg tgcgttgcgg agtatgcgcc
    12961 acggcggtgc cccgatgaca cgcgaattcg agggcggcgc gcgcgaggag ctccgtgcgt
    13021 atgtgcgaca gcagccgccg cgcggcggca tggtctggta ccgctccgac gaggagcgtt
    13081 cctatctgtc cgcctggctg gccgcaggcg atgagcgctc accccgcatc gttcgcgata
    13141 cgcccgccga ctcccccggt gtcgttcgtg acgagcgtgc ggcgtgcacg cggtgcgaga
    13201 acgtggtgcg caaacgcggg cccttcggct ccggcgcgaa cgggatcatg attatcctga
    13261 acatgccgtc gcggatcacc gatttcgaaa aaaaattcta cgccgccgac gccgacgagc
    13321 tgctcgccaa gatgatgggc gccatcggga ttgaccttgc ggagtgttat gtgaccgagc
    13381 tggtgaaatg cgaccccacc ggatccgcct catcggccgg cgccctgttc gacaactgcc
    13441 gcgccgtcct cactcgcgag ctcgaggagc gtgcgccccg cttcgtggtg gtgatgggcg
    13501 agctcgcgcc gctccagaag atcatccatc gctcgcgcgg catactctgg tacgcgatcg
    13561 agcacccggt cgtccttttg aaaaatccgg acttgaagaa gccggcatgg aacacgctca
    13621 aggtgctgcg cacgaaactg gccgaaccgt ccgccggccg ttaaggtggg gcgcgcggtg
    13681 tatgccgacg tgcacgtggg cactcccgtc gagggtccct tcacctatgc agtcccggag
    13741 gggatgacgg tcgttcccgg aatgcgcgtc atcgtcgatt tcaaccgccg ccgaatgacc
    13801 gccttcgtcg tcgacgtgca cgcgcgcatc cccgatttct cccccatcaa gcccatcgtc
    13861 gaatgcatcg atcccgaacc ggtgttcaac gagcgcctcg tcgatctcgc gcgcttcacg
    13921 gcgagccact atgtggccga ggtgggggag gtgatggcgc tggcgctgcc cgccggcacg
    13981 agccgtggaa cgcgttcgcg cgacaatacg agcgcgacgc agggcggccg cgccatcgag
    14041 ctcaacgagg aacaacgggt tgtccgcgac gcgatccttg agggaactgc gcgcggggag
    14101 aacgcgcatc tggtcttcgg ggtcaccggg agcggcaaaa ccgagctcta cctcgagctc
    14161 gcgcgactga ctatcgaggc ggggcggtcg gtcctcgtca tggtcccgga gatatcgctc
    14221 tcgtcgcaga tcttccggcg gatccacgat gttttcggcg gcgacctggt cttgtatcac
    14281 agccgcctca cccccaatca gcgcttcgac aactggatgc gcttctttca cggcgaggcg
    14341 cgaatcgcgg tgggaacgcg ctcggcggtc ttcatgccct gcccggacct ggggcttatc
    14401 gtcatcgacg aggagcacga cggctcctac aaggagaaca gcacgccccg ctacaatgcg
    14461 cgacgcctgg ccttttaccg gagccgcacg gagaatgcgc tcctggtaat gggttcggca
    14521 acgccgtcgg tggagacgct gtattcgacg gaacgcgggg tgatgcggct gcaccgcctc
    14581 acggcccgct acgggaccgc cgagcagccg gagatcgaca tcgtcacggt caaggcgcgc
    14641 gacggacagg acgcgctctc gtcgcgcctc aggcttgcga cgcgccggac gatcgacgcg
    14701 ggcgggcagg cgatcttcct gctcaatcgg cgcgggttcg cgccggtgat gatctgcgac
    14761 gagtgcagcc agttcgtatc ctgcccacag tgcagcatca gtctcacatt gcacaatgac
    14821 aaccgcctgc actgtcatta ttgcggccac ctgcagggtg cgcctgagtc ctgtcccgcc
    14881 tgcggggctg aggcgctcgt gtcgttgggc gcgggcaccc agcgagccga ggaggccgta
    14941 cgcgcggcct tccccgacgc gcgcctggcg cgcatggacc tcgacagcac acgccggaag
    15001 aacgccgcgg ccgacatcat cgccgccatg gaggcgcgcg agatcgacat tctgctgggt
    15061 acgcagatgg tggccaaggg cttcgatttc cacggcgtga cgctggtggg cgtgctcctc
    15121 gcggacatcg gcatgagcct gccggactat cgggcgacgg agcgcgtttt ctcgctcctg
    15181 gtgcaggtgg ccggacgctg cggacgcggc gccgaggccg gtcgcgttct cgtccagacc
    15241 ctgaacgagg agcaccgcct gttttcgttt ctccggcgcc acgactatct ggggttttac
    15301 cggtacgaac tctcgatgcg tcgggagctt ggctatccgc cgttcacgcg tatcgcgcgc
    15361 ctgctggtgc ggggcaagga cgagtcgcag gtcgaggccg cggtgcagtc gctcgcgcgg
    15421 gagcttgccg gcatcatcaa acgccggcag gcagcggtgc agctcctggg gccgtcaatg
    15481 gcgccgctgt atcgcatcgc ggggaactac cgcagccata tactgttaaa gtcccgtgag
    15541 gttgatcccc ttcgggcggt cgtggcggag ggcaagcgcg cctttgcgtc gcgcggcgtc
    15601 tatctcgaga tcgatatcga tccctacgac atgctctgag aaaaaacccg gttactggcc
    15661 gcccatctcc cggcgtatga tggcactgag gagctcgacc ggggggacgc cgaccatccg
    15721 cctgccgttg atgaacaggg tgggcgtagc cgagatcccg agcttttccg ccagccgcgc
    15781 gtcccgctcg atgatggccg acacggcggg cgaccccatg agggacatga agcccggatc
    15841 cggggcgatg ctgtccgcca gcgcgcgcgc ggtgtccgcg gtgtacccgc cggagatctt
    15901 ccgatagtat ttgaagtgca gtgagagata gcgggggaaa aaccccttct gcgccgctgc
    15961 gctcatcgcg cgcgcggcgc tgcaggaatg cggatatgta gtccgtttcg cgaacggatt
    16021 gcagtcggtg tccagtgggt aatggtagta ggtgaccgcg agagagccct tgaactgcgc
    16081 aaacaactgc ttctcgacct cgaaaagatg agagcatgcg gaacacagga agtccgtgaa
    16141 ggcgatgatc cgaacccgcg catttgccgg tccgatcgta agcgcgctcg gtggcagttc
    16201 caggcgttcg ggggcggccg cgtagaaggt ccgcaggtgc tcatcgaccg ccgcgcgcgg
    16261 tgccgccggc gtgaaggtct tgaggaagag aatataattg aacgagaaca cggtgaacag
    16321 gagcaggaag ccgaagatca cgtagagcga tacggccgcc cgacggtccg gccccgattc
    16381 atcggcgttg acgagttggc gaacggtgcc gcgaaggccc cccggcgcgt cgcgaagggc
    16441 gaaccggtac cagagaatgc ttgtcgcaaa catgagcgcg gtcacgccgt aggtggcgat
    16501 gcagaagccg caaaacaggc ccgtgacgac gaggatgatc gcgagcgcaa tatcgaccgc
    16561 gagcgccagc gccgttgccg gaaaggcgat gatcgccgcg tacgtataat accgttcgcc
    16621 cgcataatcc gcgacgagca cggtaaacag gataaacaaa tagaacagga ggccgatccc
    16681 cgcgatcgga actccgaaca cgctggagta gggcgagttg cgcagggacg cgcatgcgct
    16741 gttcaggtcg ttgccgcaga ggcgcagggc ggagccgatc tcgggatagt agtgctcgac
    16801 ggtgagatac gcgctcaggg cgacaccggc gatcgagagc gcgagcatga catagatgaa
    16861 ttttttcatg ccgcatgatc ccatcgggcg cgctgcgtcg tcaattacta aatccgaatt
    16921 tttcgcgcgg gggattgtaa tacccgaggc ggatcgccgg atgccgctcc cgcagcgtcg
    16981 cgcacagtgt cctgatgccg atggcgttcg tctcctcgac gccgattgcc gcccagtaca
    17041 ggtccgggtc gatgttgagg ttccgcgtct gtatcatgtg caccgggtga tcgcgcagga
    17101 aggcgaaaag cgaatcgacc tcgctttcca tgtcggtgaa acccggcagg aagaacaggt
    17161 tgagcgatac gaacagaccg cacccgagcg ccgtttcaac cgatcgacgc atgtcggaga
    17221 aggaaaacga cgacgagcgg acatagcggt tgtagtagcg ctccgtgggc gagttgaggc
    17281 tgatgcgtat cgagtccagg cccgccgccc gcaggtccgg gatccggtcg gggagcgatc
    17341 cgttcgtgtt caggttgatg gtgccccgcg cggtgtgcgc acgcaccagt tcgatcgctc
    17401 gcgacagatc gcgcgcccgc agcagcggct ccccctcgca tccctgcccg aagctcgcga
    17461 cgccgtccgg cacgcgcgag aagtgatgca gaatcacctc cgcgatctct tcgggcgtcg
    17521 gcgagaagtc gagccggtcc tgcgaatggc aaaacccgga gtcctcctgc aacgacaggc
    17581 agccgacgca ggcggcattg cacgcgggcg tggtgggaac gggcgcctcg aagcgcccca
    17641 ggaaaaaatt gcgcgcgcag aggcagcggt actcggtgga gcaatgcgca agttggcgaa
    17701 cgaggcgatt gtccggaagg cgttgtgcga gcgaccgttg ggcgcgtgta agcgccgcat
    17761 cgtccaggtg cagctcggga tcggatcgcg gatcttcgtc gatccgcatg gcggggacgg
    17821 cgtactcccc gtcgacgatg gcgacgccgg catatgccca gagcggaagc gtcggcgcgt
    17881 cggcggttac ggtataaccg ggaagaaagg tccggagaaa ccccgagctc aggaacgatg
    17941 cggcggcgac aatcgggacg cccgcgtcgt catcgggaat acactcgaat gcgtcggtcc
    18001 tcttcctgcg cgccacggga cggcgtcccg ggagcgagag gatgtggcta ccgaaaggga
    18061 gctcgatgag ctcggcggga tcgaccggaa cgaagcgggt gccgctccgg aaggcgggtt
    18121 cgagcgacgg gtgttcgtag atgcgatcgc gctcgtcgca atagacgagc tgcgggcggt
    18181 tacgtcttcg tggtgtcatg gccgatccgg atcgcgttcg cgcgatgcag ttttttttca
    18241 atatgcctga tccgcgtcga aaggcggtgt tgctccttga ccagtcggcg gtacgcgggc
    18301 gatcggcgaa tcgtcctcgc gtcgatgccg cttccctcga gggtgcgctt gatgccgagg
    18361 cgctccatgc ggacttggcg ccaggacgcg cgcaggtccc ggagttcgtt gtggagcgca
    18421 gtcgatgtac gggatgattc ttccatgatg cgggcgcagg atcgcagtgg cggtgcgcgg
    18481 gacagggttg ggcgcgctac tttttcccgg tggatcgggc gatatcgatc aagtcgccgt
    18541 cctggaggtg aatggccgga tagctttccg gcgatacgat ggcgccgttt cgctcaacga
    18601 cgattttatc gggattgaaa cggtactgga caaccaggtc caagagcgag gtcttgtaga
    18661 actcgatggc gataccgttt accttgattt tcatctcgct agctccattc gtagatgaga
    18721 atggccggca gaacgatggc cgccgtctct gcgcgcatct gcgttccgcc gagaataacc
    18781 gcctcccatc cggcgtcacg cgccgcggcg cattcgcgct cggaaaaccc gccctcgggg
    18841 ccgatgagta cggtcccggc gcgcccgtcg tcgcccaaca tcggacgcga cctgaggaac
    18901 tccttgaggt cccgggcgcg agggtccaga tgcgcgatca ttcgaacgct ttcggcgaag
    18961 cccgcgatga cctcgtggaa gtcgcgtatc gcgaaaaccg ggggcggcgt ccctcgctgg
    19021 cactgctgcg ccgcctcggc ggcgatgcga cgccaacggt taaggcggcc ctcgccgtcg
    19081 tcctccagcc gcgggatgga cctctcggtg atcatcggct gaatgcccgc cacgcccgcc
    19141 tcgacggcct tctgtactac caggtcgaac ttcttcccct tgagcagggc cacggcaaca
    19201 acgatattta cacttgaaaa gcgcgtgtcg attttgtcaa tgatttccaa aagcgcgttg
    19261 tcggcggata tctcgactat cgccgcccga taggcatcgc cgtgtgcggt cgtatactcg
    19321 acgatctcgc ccgggcccgc gcggcggacg cgaacggcgt gatggaagag atcgccgtcg
    19381 atgcgcacgc gcccgtcgac gatcgtcgga gatggaatat agaatcgcgg catgccgaac
    19441 cctccgcaca gattcgtcaa ttgccgtcgg aaagattttt ggtcaatact aatatttttt
    19501 gcttgatggg tacgacgcga tcgggaccag ctccgctatg aaaaaatatc tcgtcctcgc
    19561 cgccgcggtg tttgtcctgt tcaccgccgt gaagctgcat tttatctcgt cgaccatggt
    19621 gatcgacgac gaggcctact acggcatgta ttcgcggcat ctcgcacccg gctacatcga
    19681 ccacgggccg gtcgtggcgc tcttggtgcg cgcctccacg gccgccttcg gcgaaacggc
    19741 cttcggcgtg cgcgccggct cggtggcgct cctgtcgctg ctctcgatcg cgctcttctg
    19801 gttcggggcg cgcttccatt cgccgcgcgc cggctgggcg ctcttcctga tttcaatgac
    19861 gacgctgctc tatttcgccg ggtcggtcgt cgtcaccccg gatacgccca tggtgttttt
    19921 tatgatgctg gcgctcatgc tgtattatgc ggcgttcttt tcgcaccggt attatttcta
    19981 tcccgcggga atcatgctgg ggcttgcgat gctctcgaag atatcggtgg tctttcccgc
    20041 gctgggcgtt ttcctctttc cgctcattgc gccggataag cgggcatttc tccggtgccg
    20101 cgaattctat ctgtcgttca tcatcgccgc cgtcgtcttc tcgccgtttt tggtgtggaa
    20161 tttccagaac gacctggcct tcattcgcta ccagggcgcg catgtgttcc gcccgggcgg
    20221 cattaaaagc ttcttcgatc tctggggcgc gcaggcgggg ctgttgtttc ccatcctgtt
    20281 tttctttgcg atctatcttc ccggccaggc gatcgtgaac cgcatacgcg cgagggcggt
    20341 cgatccgcgc cgtctctttt tcgccatcac cgccgccgtc cccctggggt atttcatggt
    20401 ccagtcaatg tttagccgct acgaggccaa ctggccggcg ccggccttct tcggcgggat
    20461 catgcttgcg gggatgcaca ttgcggaaca ctgggagcgt cttcggacgc tgtttgccgt
    20521 gcaggtggcg gcttcgtgcg cgctcatcgc cgtcgcggtc gtccaggtgg tgtattcgcc
    20581 catagcgttt ccggccggga aggatgtcac aaaccgctac tacgcctacc aggcgcttgc
    20641 gggtcccgtg cgggagtttc tcgagcggga gccgcgctat cggtcccttc gaatgatgtc
    20701 ggccaactac cagatcccgt cgatggtcaa tctctacctt cgcccggacc gcgaggccgt
    20761 cgccatatcg gttgccggct atcacgacac cctctattcc ttcgtgtatc gcgatgccac
    20821 gctcgtcggt caggactacc tgttcgtgtg gctgggcgag gagatcccga acaatatccg
    20881 acgccatttc aactccctcg agagggtcgc gacgtttccg tccgtgcgcg ccgggcgcgt
    20941 cgtgggcgtg gtaacgctct gggaggcgaa gggataccgc ggacgggcct tctagaagta
    21001 cttaccgaaa aaccgttcga tcccgcccca gaggcgctcg cgcagcgcgg aaatgcgcgc
    21061 ggcgtccacc cgcatcgtcg tctcgttgaa atatcgctcg gtgaaaaaga gcgagcggga
    21121 caccgacagg cgcagcatcg gtatgccggc gttcccgtag cggttcagga tgtgcccgtt
    21181 gaagctcggc gtgttgatgg cgatccgtcc ggctacgctg gccggctcgc tctcgaagaa
    21241 tttctccagc gcctgtttga gcgcgcccgc gagttcgtcg ggacaggtcg tgtagcggcc
    21301 ggagcggccg tgcgcggcgt tttcgatggt caccagtggg cgcgggcggc cggcgtcggc
    21361 ggccgacttc gggcccacgg gcatcatcac gtggcagtcg atgatgagcg catggccgac
    21421 cgactgcgtc gccgcctcga cctgcgtgtg aaagggaagg tggtagcggc ggatgaggtt
    21481 tgcgatcgcg atctcgtcgg ggaaggcgcc ctcgtcgaac acgggcttgc cggccggcgt
    21541 ctcacgcttg atgacgccgt cgtccgagcc gggcgggagc agggtgtgcg gacggtccac
    21601 atccacgaac agtcgcgaga tgtccgtgga gatcaccgtc gccccgcggt ccccgaaggc
    21661 gaagaggtcg ttggcgcagg aatcagcgct gtagaacaga tcgaagcggt tcacgcgctc
    21721 atagcccttg agctcgtcgg gaacgcgata gcccccgtgg gggacgatga tgaggatcgg
    21781 caacgatttc gccatggcgc attatgtcgg atattttcca ttctggcaaa ttattttctt
    21841 gaaataatgc atttcctcgc caatggtgta ttcgcgcctg cggcgccatc atgcttccgc
    21901 gtttggtgcg catgatgaac gccatataga tcgagcgaaa accgggggac catgaagcca
    21961 tataagatcg accagctcag cgtgaacatt ctggagatac tcaacgacaa cgcgaagacg
    22021 tcccacgccg atatcgcccg ccagctcgcg gtgaaggagg cggaggtggc caaggccatc
    22081 aagcggctcg agaaggaccg catcatcgtg cggtacaagt cgcagatcaa ctgggagcgg
    22141 atcaagcatg acgaggtgcg tgcgctcatc gaagtgaagg tcacgccgat gcgcggcgtg
    22201 ggcttcgacg ccatcgcgga ggacatctac cggttccccg aggtcaattc ggtctacctg
    22261 ctctccggca gctacgacct tctgatccag gtggagggcc ccaacctgcg cgaggtgtcg
    22321 ctcttcgtct ccgagaagct ctcgaccatc aagaacattc agtcgaccgt gacgcacttc
    22381 atcctcaaga aatacaagga ggacggcgtg gtgatggtgg accgctcgga atcaaaacgc
    22441 ctcccggtga gcctctgaac gccccgcttt cgctcaatcg ggtctttcgc gcgctcctgc
    22501 gcgcatacgg gccgcagcac tggtggccgg gggacactcc cttcgagatc gccatcggcg
    22561 ccattctcac gcaaaacgtc gcctggacca acgtggaaac ggcgatcgcg caactaaagg
    22621 agaagcgcct gctcgccccc cgcgcgctgg acgccatccc ggctccgcgc atcgccccgc
    22681 tcatacgctc gacgggctac tacaaccaga aggcgatcaa gctcaagaac ttcctcgcct
    22741 ggtttgcggg gtatcgttac tcgtttgcgc gattgcacgg tgtcgatacc gagcggctcc
    22801 gacgcgagct tcttgccgtc tcgggcatcg gacccgagac cgccgactcg atcctgctct
    22861 acgcgctcgg ccggaaactg ttcgtcgttg acgcctacac cgtacgcctg ctgggccgcc
    22921 tggggatcat cggcggcggg gaatcatatc atgatgttca gggtttgttt catcgccgct
    22981 ttcggggcgg ggtgggggag tacaacgagt tccatgcgct tatcgtgaat cacgggaagt
    23041 acgtgtgccg aacgaggccg cgctgtgcgg agtgctgcct gtcgcggcgg tgcctagcgt
    23101 accaccagca gggagctgat gccgcccatg ccgtcgccgg atgatttcgc attgtagatg
    23161 tcgggccgcc agacgccgtc aatgatgtac tggtaccggt aggttcccgg cgacaggcgc
    23221 ttggtgagcc gccagaaccc ggcgctggtt ttcttgagga cgtcgttttc cgggttccat
    23281 gcgttgaagt cgcccaccac cgcgacatag ctggcgttgg ggtggtagca acggaattcg
    23341 acggtgcccc ggccgatgat gcggtagctg tcgtggcggt tttccggcct cgttcccggg
    23401 gcgatgcgcg aaacgaagga gccggcgccg tcatcgatgc ggtcgggatt gaggggatcg
    23461 gcgatccaga tgccgtccac cgtgaattta tagcggattg gcggcgcgga cgagggggcg
    23521 tgttcgctct cgttcccggc cgaatcgccg acggcgcggt ttccgaacgg gaggaagtga
    23581 taccagacgc ccgatgcgct gcgtgtcatg ggaacgggct tccaggcgtt gaagtcgccc
    23641 gcgatccgga cggatgcggc cttccgattg ctgtaggtga cgagggtgcc gcggcggatc
    23701 acccgtttgc ccagccgggc attgtcggtc ttgatcatgt cgaccacgcg gggcggcgcg
    23761 gcctgtttga gcgactgcag cgcgtaatag tattcggtcg gaacgccggt gtcgccgcga
    23821 tctcccgtcg ggggggtgaa ggcggtgaac aggatcggca gcacgcaggc gagcgtcttc
    23881 aggcgcggga tgagcggtat catcaagtac cccgagaaac catatttcga tactatgaat
    23941 gtcgataggg ccgcccggat ttctgaaaca attttccgcg ccaatgggat gcggtctcat
    24001 ttcgcgacgg cgataatgac ggtggcgggc gctttatgta ctttataatt gtgcaaaaaa
    24061 tgtcgatagg tacaggaagg ggaggcctct caggggaccg tcgtgtgggc ggacccgggt
    24121 gcggctcccg actgcggcag ccaggacagg accatgggcg ataaagaaga acgaatatcc
    24181 tacaccgcgg acgagcagaa ggagatcgac cgtattctct ccttcgtcga taccgagacg
    24241 gaccgcctga tgtccggatt cacgaagacg gccacactgg agcgcgagag cctacccgag
    24301 gagttccaag acgacgatcg ggaggccggg gcgtatcgcg agatggagga cgatgtcgcc
    24361 gatatcgacc gggacatgcc ggaagacgag gacatcgtgg acatcaccga tatggtccag
    24421 gaggtggagg agccggcgga gccgtccggg gccggcgaat tcgacgatct cgattcgctg
    24481 gtacgcgatg atgagcggcg tccccggtcc cctgaagcgc gagacgaggc ggacgagctt
    24541 gatacgctct cgaccgcaga cctggatgag gttgccgacg aaaagcccgc gttcaaatac
    24601 aagccaaaga cgaaacccgc ggagggcgat gaagagccct caaccctcga tgaacttgaa
    24661 gcgcttgccg ccggcgaggc ggtatcgggc gacgaggccc gagagaacga ggcggtgccg
    24721 gccgatggtg acgaactggc atcgttcgac gatttcgaca tgccggcgga aaagccacgc
    24781 gaggcgggga agggcgtgag catcggcggc gacgccgaat cagacattcc cgatctttcg
    24841 gacatctctt ttgacgaggc cggtgagatg cccgaagcga aggacgagga cataccggag
    24901 atcgatctcg gcgggctgga tgatttctcc gccgctgaga aaaccgtccc ggaaaaatcc
    24961 cgcacggccg cggacgaagc gccgatgtcg ttcgacgagg aactgccgtc attcgatgac
    25021 gacgcccttg ccgcgccatc ccgggcggaa tccgagatcg ccccccgcgc cgccgagaaa
    25081 cgcgcagtcg ccgacgatga cggtttcggc gacatcccat caattgacga aattgacgac
    25141 atcgtcgcca aggaggagcc ggccattgcc gccggcgcct acgatgatga ttttggcatg
    25201 gccgaacctt cccagacgaa ggccgcgtcc tttacggaac ccgctcgcga acgggaaagc
    25261 gcgccgccga aggaaaagcc cgcaaagggc gcgcgtcgcg aggagcagcg cgacggcctg
    25321 gacctgagcg atcgggatct gaaaaaactg aaaaacggcc tcacgctcct gcaccccaac
    25381 ctccgcaagg cggtcaggga catcatcctg aacgacgtat tgcccgagca ggacgcgcgc
    25441 caggtgctgg acgcgatcat cacgggcaag tccgaagatg cggtgctcaa gatcgtccag
    25501 aacaagctgg gtaaaaccat cgacatatct gagcccgccg caggcgccgc cgggcgccgg
    25561 gtcatcgccg gccgtcccga gtacaccaag gacggcatcg agcgccagaa aaagctgctg
    25621 tttctcacca agatattcgg cgcggccgcg gccgtcacgg cggtcctggt ggtcgttcta
    25681 tatcaattct ggtacaagcc gtacatggcc gagcgctaca tcgcgaaggg cgtggcgctc
    25741 atccgcgagc cgggcgatcc ggtcaccaag aaggtgaagg actataccaa ggcggaggag
    25801 ctgttcacgt ttgtcgacga gaactataaa aaggactacc tccgaggcta caacgaatat
    25861 gcgcgcgcct atttcgacaa gcgcgagttc gacctgtcgc ttcggaagct gaacgccgcc
    25921 taccgcatcg atcccgttca tccggagacg ctcaattcgc tgggatattt cttttcgcgc
    25981 atccccgaag gctacttcaa gcagatacgg ccgcgcctca aggactggta ctacaaggag
    26041 gttccgccgg tggggtccgc cgaaacccag ctcgatgtcg cggtggactt ctatcggcgc
    26101 gtcctcaatc gcgagcccaa gaacatcacg gcgctcttcg ggatcggaaa cgcctacaag
    26161 taccaggggc aatacctcaa ggcgcgccag tactacgaga acatcctgaa aatcgaccgg
    26221 aagtccgtgg tgggctactc ggggcttctt gacctgttta tcgaccggga caatttcccc
    26281 gaggtcctca cggttcactc ggacctgcag gacaaggatc tgatggtcaa gctcccgtcg
    26341 ccgctcttgg cgaagctggc ctcgtactac ctgggcaaga agcgcaccga ccggatgaat
    26401 atccgcgtcg attacggcgt cgagtcgccg cgcctcaagg acatcgcgga caacccgtat
    26461 cccgtcgtcc ggtccgtgct ctcggccctc gccgagcggg acgccgacta tccgccgctc
    26521 tacgtccatc tggccctttt ggcgcgcgcg caggacaacc ccaaactcat gatggagaac
    26581 ctcaagcgcg cgatcaagcg ggccgaggcg cgcgaccagc agtatttcgc cgcctatcac
    26641 ctgatgggcg agtaccacct gcacgtgaag gagccggtgc aggcctacca gaacttcaac
    26701 cgcgccatca aggcctattc ctcgccggcg gacttcatgc gggaggattt ctacgccgag
    26761 accgagagcc tggggaagac ctatgcgatg atcggcaacg tcttctacta tttcttcgac
    26821 aaggtgcgcc accgattcgg cgacgagatc gaggaccgcg agctcgatca ggacgtcgag
    26881 aagttcgcga actatcgcat cgcgcgcgac aagtacgagc ttgccgtgca ggaggggtat
    26941 aagtcctccg agctcttcta caatctgggc cgcatctact acatgaaggg cctgttcgag
    27001 aactacgaaa aggcgctgga gagctggctc aacctgtacg aggatttttc cgcgcggccg
    27061 gagctgatgt acgccgtcgg gaacgcgttc taccacttgg acaacctcga ggccagcaag
    27121 ggcgagttcc tcaagctcat atcggtgttc gagcaccagg ccgagcgcct cacggccgtg
    27181 catccggaga aggccgcgca cgtccggatt ttccagacgc tcgcctcggc gtacaacaat
    27241 ctgggcgcca tctaccagcg gcagaacaac gagagcaaga gcaccatctg ctactggaag
    27301 tcgatcgact atgcggcccg cctgcggcgt gaaagcgagt ttgcgcgggt gaacctcgcg
    27361 cgctcgttca aggcgcgcca ggaggagatt tcgccgatca tcgacgaaaa cgttccgtac
    27421 gcgatcgaca tctacagcga ggacatgcgc aagtagcccg accaaaaaat cagccgcagc
    27481 caaaaaacca ttggaaaaaa tcctcccatc gggtgtgatg gcacccgcgg aggacatgca
    27541 tgcagcggtt gccgatcctg tatctgtata agaaggtggt gttcccctcc tgcgcgctca
    27601 cgaccggtac gcgggagaaa cggcaccggg gccttgcggc gggcgatcgc gtgctcgcgc
    27661 tgccgatccg cggatttttc gacatcctgc ttgcccggcg cggcgtcgcc accctgtcgg
    27721 aggtgacatc ggtcgaggtt gccgaggaca ccgtgaccgt gggcctgaag ggcatatcgc
    27781 gcgttcgcct gcggcggatc gaccggttcc tgaccgccga gcatgtcccg attccggaaa
    27841 acggcgcctg ggcccgggac ggcatcgtga acgagcttcg aaaaaaatcg caggagctca
    27901 tctttcttat caacgtcaag gagtcggaca agctcatcgg gctgctggga ttcatcaaca
    27961 atccgagcca gctctccgat ttcgtcgcga actattttat cgtgagcttc ccgcgccggt
    28021 atcgcatcca tcgtgagatc gacggcgccc gacggctccg caatcttatg gccgtgctcg
    28081 acgagctaat cgcgaaattc agggaaaaga gggaagccgg ggtctcatga aaaagaaaac
    28141 cgacgaaagc ggcgcgtact acaccatcat gtggtcgaag atgtacaagt acgacaaata
    28201 cgaggcccag cgcatcctgc cggagctcac cggcatccta tgccttctgg agaagaagga
    28261 tagtcgcgat ccgcgcccga tcctgtttta taactgctgg cgcgagggac ttaagatcgc
    28321 catcaagaac ct
//
LOCUS       DAORHF010000018        28009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1032_length_28009_cov_5.390785, whole genome shotgun sequence.
ACCESSION   DAORHF010000018 DAORHF010000000
VERSION     DAORHF010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 28009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28009
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1032_length_28009_cov_5.390785"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKJ16_02905"
     CDS             <1..306
                     /locus_tag="PKJ16_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HNV45963.1"
                     /translation="VDGIEFEKICEMLDRHIQKVLREVDTQDLAIALKGTSAELQGAI
                     FRNMSPRASSLLREDMDFMGPVRREDVESRQEKILNIVIKLAECGDIIIPNGSEFIE"
     gene            517..939
                     /locus_tag="PKJ16_02910"
     CDS             517..939
                     /locus_tag="PKJ16_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45964.1"
                     /translation="MQIQYPGTEAIHSALQSIIKDCFWDSAVSEDDIRTIVGTDQVRL
                     KQKLFAKIRFHVHGRYSDDLDFFAPDDLRFGENIKQVLDALTGNGVRFARVICLERNA
                     GDSPKESACREGTPHRADPRLSPGLVVEPENTATAYSG"
     gene            complement(1123..2163)
                     /locus_tag="PKJ16_02915"
     CDS             complement(1123..2163)
                     /locus_tag="PKJ16_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNV45965.1"
                     /translation="MMTAPYEQYADSRDVWVFAEIQDHERVLEGALELLTRGRELADA
                     LGSRLIALVFALDAEQYLPAIERHGPDAILCCSHPALKHYDSQIVPDMFADLIAERRP
                     SIVLFPSTEAGCDLAPRLAQRFATGLTSHCTDLSIMDHPDYGPGLLVMKRPAYSGNAI
                     ATVICPHARPQMATVQQGVFERRERDDVATDIEMLPFSYDLTKLRIESLEAPLRWDKP
                     KVPLEQAPVVVTGGRGLATKKNFERLFELAELLGGEVGATRVPVFNRWCGEERMIGQT
                     GKTIRPRLYLGFGISGQIQHTASIVDSEIIVSVNTNAGAPIAALSDYVVTDDAGVFLA
                     KLIERLRSEKRG"
     gene            complement(2171..2962)
                     /locus_tag="PKJ16_02920"
     CDS             complement(2171..2962)
                     /locus_tag="PKJ16_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNV45966.1"
                     /translation="MLSIIVCVKQVPDTQKAVFDPKTGAIDRSATGNIMNPDDRHALE
                     MALALKDAHGATLCALTMGPPHADEVLYEACALGADRGVLLSDPRFAGADSLATSRAL
                     ARAIERLGPFDIIVTGREAIDGNTGHVAYQLSEFLDAPLVSQIHRIRIDGAHAIIERQ
                     YGHEYQTIRCPLPALLSVGSRDNRVRHPRLADIAAGAVQPIRRMTMDDIGGSESDYGA
                     SGSPTIVIGAELFSHTRGREALPGGTAEKVEALVHRLKKHNILRY"
     gene            complement(2968..4125)
                     /locus_tag="PKJ16_02925"
     CDS             complement(2968..4125)
                     /locus_tag="PKJ16_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV45967.1"
                     /translation="MDMALDRTQLMVQRAVRAFARETLEEWANVLDRESRPLPAEIVA
                     EMGRINLWGIQAPVRYGGAELDTVSYAIAIEEISRVSAAVGLGVTVHNSVCLAPLLKF
                     GTPDQIERFVPDLASGRKIGGFTVTEPQAGSDAASIQTTAAPDGDSFIINGQKAFVTN
                     GGVGDLFLVAATLDPAKKARGIAIFIIEKSMPGFQVGKIEDMMGMRGNMVSELFFNNV
                     RVPKDNILGRPDEGFRISMQTLDIGRIGIAAQALGIGEAAFAAAATYAAERTQFGKPI
                     GTFQAVSFKLAEMKTRLDAARLLVCRAACLKDRGLPFSTEAAMCKAYASDAAMRVCRE
                     ALQVFGGYGYVKDLPVERFFRDAKITEIYEGTSEVMNIIIGNAIMKEFGRM"
     gene            complement(4133..4828)
                     /locus_tag="PKJ16_02930"
     CDS             complement(4133..4828)
                     /locus_tag="PKJ16_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009054542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNV45968.1"
                     /translation="MMTPARTYPRVLIALIAVAHLLCADTAPARDVDTVRILDRPIPF
                     GETRRRLTLEYARLHYDPATTDIAITPRMIVVHWTATRTLQEAYDTFAPEILPPSRPE
                     IARGGRVNVSAHFLVDRDGTVYRLMPETLMGRHVIGLNHCAIGIENVGGPANPLTAAQ
                     LAANARLVRHLANRFPTITYLIGHYEYRRFERHPLWIERDPAYRTVKVDPGAAFMTRL
                     RERVLDITGLAPF"
     gene            5012..5815
                     /locus_tag="PKJ16_02935"
     CDS             5012..5815
                     /locus_tag="PKJ16_02935"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HNV45969.1"
                     /translation="MVLLCIASGCDKAGKRAGAKNEITVATYNIRYCAAGRGPVLNAI
                     RSLGADVIALQEDPVRGGESAGSAFARALGMHHASSAPYAVENAMPWALSILSAHPII
                     SRDELRVKSGRRALRVRISVGGAGCELVTTHLSPFVWDATNPVAANRARSLRRREELR
                     ELRAWLGTPSTPRILVGDFNSLPVMGELAVLADDGYRSVWDALDMPGQGTFRLSASVH
                     SFVKKYLPVDEEIMLDYIFVSVGITVLQAGVVRSDASDHYPLTARIRLP"
     gene            6150..7061
                     /locus_tag="PKJ16_02940"
     CDS             6150..7061
                     /locus_tag="PKJ16_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45970.1"
                     /translation="MPENRLKVPLAVSQDLVKHIRLLALAGRRSFDRYLVGPIREAGW
                     RPITSAQKTRKMIERIGIEMENPAYRHTISPRCKRLVQVSLSESMAAVGDASIFFLES
                     MQADAIVASGEESIEFADIIEPPLAEFREVNSTKSERLFRETIEAIDGEELKTAFDPV
                     RLGNPEQKVQMDREVHSLYRQIVVASGRNDIPKCRKLIAGYLIRSSDREEYDAHEVEK
                     VIDALEQREPGFRRDLMDMLEADLYYRITRGIIEGDVAKAIQGIRKYSYVFQGSPDTR
                     HYYDVDRLERALYQIITDKNLWDDLKK"
     gene            7091..7438
                     /locus_tag="PKJ16_02945"
     CDS             7091..7438
                     /locus_tag="PKJ16_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV45971.1"
                     /translation="MAEPTLTLKDMTQGAVSVLAIAGELDAKTAPQLKAKLDAIVAGG
                     VVKIVCDCAGLKYISSAGIGVLNAALSACKAKGGDIALAGVSKQIKDTLDVMYFTKKV
                     RLFQKIADAVNEL"
     gene            7452..7883
                     /locus_tag="PKJ16_02950"
     CDS             7452..7883
                     /locus_tag="PKJ16_02950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV45972.1"
                     /translation="MAGAIEKRVIFTNDLDELSRIRDEIQDFIGDCLEYVERNRVILA
                     MDEAIANIIKYGYPNGGRGTVELVMRRDDVRLEFVLTDDAVPFNPLELPDADLEHYHN
                     TGASGGLGVYLYRRLMDVSYEPTPSGGNRLTLSRRINEGDS"
     gene            7880..10750
                     /locus_tag="PKJ16_02955"
     CDS             7880..10750
                     /locus_tag="PKJ16_02955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV45973.1"
                     /translation="MKKKWYRALVPGIRIKLTLVTTLFVSLVIAVTSFFHYRQQRQTL
                     EEAMEREAAAPLRFVTALAVERENLKRGIVSLEELRLRIQEKRKELGRYRVAVYRRKS
                     TFGNTMRRFGSWLGMKVAYDYQHRSVDTHYSRYLSDKDITVLEERIKAQLLAGGSRAP
                     ADDLAKLKAAARPVAVSLLAIAALEAQAESAADAEAKRFARLMEGEEKKRAAAERSLD
                     STLNATLGRGYQSRAAEQGLTGDSVRIQSINPGGEVYFDTGSVFGRTTYRFDRLFRND
                     AYAAGRKSFIAEADPVSLLADGPRRQSYRVVDSHFEVTYRPVFKNPFIEEMARAMLVE
                     LRTNRAAWKRFLDDDRRISESIAQVAEKLTARVQALRKTPLVKPAADREYRSLYRDYQ
                     ALVKQRAQSFAANNPHAKTHDQRAAAIAAERTALDARDKELRAAVGAFTPGQTVRVAP
                     DGIEVRLEALLAERDRLKRQSAELAARRDDLRDSPERFADAIRHVRDAVVFSYVMLPY
                     RVDPLQHERYLAGKASRDALQLRWAALRAWIAEGASETDTPSVVVGGRREPLMADGVL
                     ARSRSEAEEFLWKIDSLPLADDEGDHDGGIIALIVADGHIGYNAIFTDKTDGMRAIID
                     NRNEILRYGVTIALVAVVAIFLLSSLMVRRIRRIIASAELVGEGDLEVQFPRAGSDEI
                     GTLSESLDEMVKGLRDREEMRGEMVAAEEIQRRLLPEAMPAVMQERLLCRTFYKAMRG
                     AGGDYFDFIDSGEDDFYFCIGDVSSHGAGPAIVMALLRAQLRGIIRRGGRDMKKILTE
                     INESVYADTPPHIFVTFVLLRCTVSTGEIAYCSAGHCPGLVYRANSTTVETLEGRGLP
                     VGMDESELFETTIKNRSFRLDPGDMLFLYTDGLTEAMDPHRELFGEERLQGIIAEQGG
                     ATPSELVDVLVAAVERHTGTELKGEGPSQLNDDIAMIAIKRT"
     gene            10766..11590
                     /locus_tag="PKJ16_02960"
     CDS             10766..11590
                     /locus_tag="PKJ16_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV45974.1"
                     /translation="MNQITIDETKCKHDGACVAECPVGIIMMADKKSLPKIIPGGEEF
                     CISCGHCVAVCPHGALAHTIARPEDLAEVKKEWMLSPEHAEHFLRSRRSIRAYKDKAV
                     DRETLERLIGVARHAPSGHNVQPVQWMVVSGAEKIRELSGAVIDWMRYMIKEKPEIAT
                     PLHMDGVVLYWEMGRDMICRNAPHILVAHAPRALPQAATDGTIALAYLELAAPSLGLG
                     TCWAGYFHVASLYWPGMKTALGLPDGHVSLGAMMVGYPKYGFHRLPERNAPQIIWR"
     gene            complement(11621..12862)
                     /locus_tag="PKJ16_02965"
     CDS             complement(11621..12862)
                     /locus_tag="PKJ16_02965"
                     /inference="COORDINATES: protein motif:HMM:NF018485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV45975.1"
                     /translation="MHYGWIILGASVLTVFSSLGFGRFGYTMIFPAMKTGLGLTDAQA
                     GDLATGNMVGYLLLALTCGFLSAHFSPRKIIASFMALLAGAMLLTGLAPDFFTALIGR
                     VLTGMGSGGTNIPVLAIVIAWFGVSRRGLATGITVSGSSAALLFAGIVIPKILAAYGV
                     DGWRYAWFALSGIAVAIAVVCAFVLRNTPSELGLSPIGHDGSAPPTDSAAISLVDSMR
                     LVYRTPTVWHLAAIYSLYGFSYIIYATFFVRYLNWECGFTVADAGALWSAVGGISIAS
                     GFIWGTVSDRMGRKYGLSCVYAIQFACYVLFGLWKSAPGIYASALLFAVTAWSIPAIM
                     AAASGDILGPRMGSAALGFITLFFGIGQALGPFMAGRVADSFGSYTLAFTIAGGASLV
                     GAILSLLLHPVEQKSNQHGEA"
     gene            complement(12872..13273)
                     /locus_tag="PKJ16_02970"
     CDS             complement(12872..13273)
                     /locus_tag="PKJ16_02970"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV45976.1"
                     /translation="MPYIMWNDSFSLNDPTIDSQHQRLFDLINALHEAVMDGSRTEAL
                     AAALAELKRYTEYHFRAEEELLAHAGYPGLEAHQHLHREFIDRIDTFIARQSKKKMGL
                     FQDVTSYLRDWLIDHILKSDTEYVASLGIRR"
     gene            13401..14357
                     /locus_tag="PKJ16_02975"
     CDS             13401..14357
                     /locus_tag="PKJ16_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV45977.1"
                     /translation="MRKTAIVLAAVCLIALCGAIAACRGADTRAKPAASNEPRYRGLY
                     ISNKYSVNLPFMKRLVERGKPLGVNMLVIDVHPYKILRPRINKEVIDYLKSENLYLVA
                     RVVCFQDGLDKLPVPEPYMERLMNVFNTALTYDFPEIQLDYIRFKDGGVPYSLKRKYD
                     YIADLLQSFRTISNARGVKLSADLFGRVMYNKNDPIGQQLELFARYTDVIYPMLYPSH
                     FTGDRQRLSNPGATIKEGTMLGINRLSGTPVRVLPYVQAFGYNIGWARVNLVKYVELQ
                     VIAVEETPAGGWVAWNAKGDYESVFRALDNLKAPVASQVETR"
     gene            14435..14626
                     /locus_tag="PKJ16_02980"
     CDS             14435..14626
                     /locus_tag="PKJ16_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV45978.1"
                     /translation="MPVYEFTCDQCGIVFSELRRMGDFDPPACPECGSRDTRKIFSMF
                     AGAGSAASCGTCTTKPSGG"
     gene            14682..16748
                     /locus_tag="PKJ16_02985"
     CDS             14682..16748
                     /locus_tag="PKJ16_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV45979.1"
                     /translation="MKTAPLILVAGGTGSGKTHLIDRMLAANPGLRCAALVDSPGNAA
                     IDCAAIGRGVVVPLMQGAIILRSFPRDLDRVVTRYAPDAIVVELRAGIDLSGLLGRLY
                     RTRAVLMGVIAVIAPSGAALDENDAAALPLADLVVVNDGAPGAGDPGGIVARAREINP
                     RAEILSGGSLPSMDSMMNGPRYDIAARLRACGCDGPTGGAALRDDSGSHAIDFFDGRW
                     VEIDALRSLLASPPVPLETAKGFVCLQPDGRRYRFYHRNDTFILQEAAPFPDAQVETA
                     DWMHRHDHDHDDCDCESMETDEGSDSLAEFSAITFTVGCDDAGVVDAELAMARAMERC
                     WEYPDAGPRAGAPDDGTIETIANLMHDEKFIEAASLVQECLRSCAEIPSSLYGFLAEI
                     YDALGMPYRALTLARCACIAGSPSVETLERLARAYNAVDDCARAVTVLDRCLSLDPEF
                     ISGLLNQARLNFAYDRLDRARNALERARAMDPDNPDAIMLAGQIADESGDPGGAREEF
                     ERLAAAAPDDAEIAFRLASACNDCDDLDAALHAQRRAAEADPDDAYARYCCGFIALRA
                     GDDADALEWLEQAREGGFTNSRLFLCAGIAHTRLDEPDDAALRFADARDAAMDALNDD
                     PFVDSLIEQAILAHIAVGETDDARRRAALLAVDPAFAFSREFGQLCRMMDTFPLAHEL
                     LRMSES"
     gene            16767..18659
                     /locus_tag="PKJ16_02990"
     CDS             16767..18659
                     /locus_tag="PKJ16_02990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV45980.1"
                     /translation="MRPEFREIIRILLTRKSILAATISIALIESLLAIALPYLSRVQI
                     EVLESRILAPGAALSTPLALFVALLAAISLIRLLQAAAAGLGRVIDVYARERFTLQAD
                     ALLYGKMETMDAGFFNNPKNRRLLYVLFDVGQLPGAVLQFCKSQGRVTVALAGIFPIL
                     AVVDARILAVIAAAGALQLLVLRFRIRAENSYRLYKERRLADILEMKFLLRYHYHQVL
                     GVSGEDRIMPRYWSRAGELVALETGQERLAFRYQLVNVFVEQGALAASAAVAGAMALS
                     GALSIGAFTMVTMYVVYLQSCFGDINQSINEFYRLRSVFLQLGFFLALRPRVAVPDGA
                     PAKAAIAGDIEVRDVSFRYPVLTTEECDYLSHLVTRLNLANKRREVYGSDLSIVREWQ
                     RLLDENHAPNPLVLDGVSCRFANGSITALVGRNGSGKTTLMNLLMRAYDPERGAIAIG
                     GVPLATAHPAAARHAISMVAQAPFLLESYSLRDNLLLGAAREYDDAALWDILGELGLA
                     DELRRFPGGLDSVVGDEVNLSGGQAQLLCIARVMAQRRRVIILDEGTNQLDAENELRI
                     VGILERLKRDAAVIIITHRMTTARKADLIHVMDGGRIVESGTHVELASRDGGVYRGFW
                     EIQVVR"
     gene            complement(18916..19725)
                     /locus_tag="PKJ16_02995"
     CDS             complement(18916..19725)
                     /locus_tag="PKJ16_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV45981.1"
                     /translation="MREIISMPFFVNAIAMCVIMSLLFGMLSFFVVLRRMSFLGAGVA
                     HTAFGGVALGLFLGIDPFFASLAFCVLAAVTLGRLVSRDGLSYDAGIGIFFAFSMALG
                     AILIAVRKAYSFDLMGYLFGNILGVTTFDTVMAAIALVLLYLFVGLYLHRLLFMSFDE
                     DVASTSGVRTGALDTLLLALMAAVIITGIKVVGIILISALVVLPASFGLLLSKNFRLV
                     IAWGILYTFAIMTGGLALSFVLDTPTGATIVSLGTALYFLAMAFVRIFMNK"
     gene            complement(19718..20494)
                     /locus_tag="PKJ16_03000"
     CDS             complement(19718..20494)
                     /locus_tag="PKJ16_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HNV45982.1"
                     /translation="MERPIIALNNVWVEFGSIIVLEDITLAVRPREIVSIVGPNGSGK
                     TTLLRTMMGLTRPTRGTVSILETAPAEIMRTGRIGYLPQERRQDERFPVTAYDVVAMS
                     RFARTAVIERLTRTDRQAIEGALDTVGMADAARAHFGSLSGGQKQRVLIARALALNPE
                     ILLLDEPSTGLDAVAQDSFYLLLRKLRDERGLAIVIVSHDIGTVSMIVDTVACIKRKL
                     HFHGKPADCLGSDRLAEVFGKNVYVLHHDKNCPTCRDDHA"
     gene            complement(20510..21382)
                     /locus_tag="PKJ16_03005"
     CDS             complement(20510..21382)
                     /locus_tag="PKJ16_03005"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HNV45983.1"
                     /translation="MTPTKRIHSRDHAPHRAFFPLLIWVMLACGASGPTAEKPEIAVS
                     VFPVYDIARTIAGDRLTVSAIVPAGANPHIYEPPPSAVRALQNARLYIGISSGFDGWA
                     ARFVPTTAKTVYLLNDDTANPHIWLSVRGARAIADGIAAAYCAIDPANRPAYEKNLAA
                     YRARIDDLHRDIEAALKPVRGKSFIQWHASWDYFAAEYGLVIAGTIESGHGHKPSLRA
                     FGDLIAAGRRMSARAVIIDLNAQSAEARTIAEALSVPIIRLDAIGDPATPSRATYLSL
                     MRHNATALAAALSR"
     gene            complement(21441..21998)
                     /locus_tag="PKJ16_03010"
     CDS             complement(21441..21998)
                     /locus_tag="PKJ16_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNV45984.1"
                     /translation="MITIEEHYPGRIFKHCPRCGAGGFALRDASSFRCERCDFVYFVN
                     AAAAVAGLVFDDSRRLLLVERKNEPRKGMLDLPGGFADIMETAEEALRRELREELNIE
                     ITTATYLASFPNRYLYGGITYFTLDMGFICAATDFSRMRAHDDAAAIVFMRPDDIDLD
                     AIGFESIRRMIALARQSPSSPTSAF"
     gene            22187..22747
                     /locus_tag="PKJ16_03015"
     CDS             22187..22747
                     /locus_tag="PKJ16_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45985.1"
                     /translation="MDMGGKLICKACRLIIAESKLGAVCPACGVPRSAFEAYADPVKP
                     RRRFFIDLNLHPISLHFPQALSLLVPFCLGAGFIAGGQWGARFDGAALILSMLFPLSV
                     LGAFTTGVFDGSMRFKKLRAPYLVRKIFLGASLFMLSVAAAALAFMVGVEGARVSIGA
                     VCMAAVLVEIALAQIGKSLMCAALPG"
     gene            22865..25720
                     /locus_tag="PKJ16_03020"
     CDS             22865..25720
                     /locus_tag="PKJ16_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV45986.1"
                     /translation="MNDHSAERPEAGGGASRTEDTSFELAHIRYQELLQSICEGYYEC
                     DLSGRVTFCNDSFARMVGLPVERLVGAPYRTFIEPDDIGTVEPLFLRILKSGETERFS
                     DWRMRSQSGARVYVEGMASLIRDARGAPIGFRGIVRDISTFMAVHEDLVRSEQRFFDF
                     LESLPEIVFEMDMKGNFLYVNRRGCEVMGYSRAEIMCMNALDILRLDERERASRGIIK
                     TMLGKRYSAIEYTFHRKDGSWLPVHVYARQVVEGGEVIGIRGIAIDATELKEAERAIL
                     ESEERFRTMIEHSTEMISILDWHGVIRYQSQSTRRILGYSPEELVGRRFVDLVHDDDA
                     HQVVVTLTGSLKRMNEEIVAEYRFRDREGHYRVLESTGVNLKNSLINGILLNTRDITD
                     RKAAEYALRKREERFRSMYNNAIIGLMTIDRASTSVLTTNEIGFRIFGCESNNEFLGV
                     SLGPRFVDSDAFAQFEHEVMEHGVVNNHEIEIRRTDGSSLWTEMSGKHLTEADNIELA
                     IIDISKRKKAEEQVAYFSFYDRLTGLPNRELISNRIQTEMLKARRKGSHVFFVLCIGI
                     DRFKFVNDMSGTAAGDQVIIKAAGRLKMSFGGEDLVARLDGDKFVAVYSDAEDTGDVL
                     AAVQKAMTAFADPFVIDGSIFPITASIGVSVYPVDGTAPNTLIKNSESAMYLAKEQGR
                     NGYRLFDVQLNAQMRQRLQLEAELQRAIYQNEFVAFYQPKVLQNGRIMGMESLIRWQS
                     PTRGLVPPMQFIPIVESNGMIRDIGSLILLQSCRQNRLWQDAGHPPVKVAVNLSPIQF
                     RQPDLIDSIARIVKDTALDPRWLELEITESGIMENEKENIERLSEIHRMGISISIDDF
                     GTGYSSLSKLRDYPIDTLKIDKSFIEKLPADDKSATIATTIIDLAHNLGFMVVAEGVE
                     SSDQLAFLDDRRCDQYQGYFFSKPLPPDEFEKKLRAS"
     gene            25796..26833
                     /locus_tag="PKJ16_03025"
     CDS             25796..26833
                     /locus_tag="PKJ16_03025"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HNV45987.1"
                     /translation="MTSRKRTTANLDWKNLPFGYVKTNANIRYYWRDGVWSEGEVVSD
                     ENISISVAAPCIHYGQQAFEGLKVFETKSGEIVAFRPEENAMRLNRSCERIFMPAIPE
                     KLFVDAIHRVVAANVEFVPPYGTGASLYVRPLIIGVGPRVGLGPAEEYIFIVLVSPVG
                     PYYKGGFKPVEALVVEQFDRAAPLGVGDCKVGGNYAAGLAGSEYGKKHGFPIVLFLDP
                     REKVYLDEFGTSNVVAIHGDAYVTPKSPSILPSITNRSLAVIAEDLGMRVEWRQIAID
                     EVQYFSEIGAVGTAAVITPVYRIRYRDRDFTFGKPDVAGPMLTKLYEQLTGIQYGEIE
                     DRHGWLDVIEV"
     gene            complement(26902..27099)
                     /locus_tag="PKJ16_03030"
     CDS             complement(26902..27099)
                     /locus_tag="PKJ16_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV45988.1"
                     /translation="MARPLLGELLLENNEITDDQLNKALEVQKNEGGLIGIILVNLGF
                     ISEQKLVQYLALQAERVVKSE"
     gene            complement(27222..>28009)
                     /locus_tag="PKJ16_03035"
     CDS             complement(27222..>28009)
                     /locus_tag="PKJ16_03035"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNV45989.1"
                     /translation="DGVTIGGIAGDQQAAMVGQNCVFEGTIKNTYGTGCFMLLNIGSQ
                     HTISRHGLLTTLTCDKHGKPAYALEGAVFIGGAVVQWLRDYMKFVDSSADSQPMALAV
                     DKEDEVVVIPAFVGLGAPYWKMDARGAIFGLTRDTTREQIVRASLKAVAFQSMDVIDA
                     LQNDSGKTIHELRVDGGATANRFLMQFQADILGIPVLLPEVAESTALGAAYLTGLTSR
                     LYNSIDEVAKHNAITARFDPDMAPNERERQRRIWKQAIQRLLM"
BASE COUNT         5240 a         8506 c         9155 g         5108 t
ORIGIN      
        1 gtcgacggca tcgaattcga gaagatatgc gagatgctcg accgacacat ccagaaggtc
       61 ctccgggagg tcgatacgca ggacctcgcg atcgccctca aggggaccag cgccgagctg
      121 cagggcgcga tattccgcaa tatgtcgccg cgggcgtcgt ccctgctgcg ggaggacatg
      181 gatttcatgg gaccggtccg gcgggaggac gtggaaagtc gtcaggagaa gattttgaat
      241 attgtcatca aactcgccga gtgcggcgat atcatcattc cgaacgggag cgagttcatc
      301 gaataggacg tgcgttgatg aagctggttt gatcgtatgc tcgtccaggt ccggcggttg
      361 caatcgcgtt caccgtaccg agtgattttt tttgaaaatg atacaacatc gagcgggaga
      421 atgaagtgcc cacggtgccc gatgggtcaa aaagctcatg acggtgctat cgtggtggca
      481 gtacatttta gatcgggacg atgtattgat tgatcaatgc agatacaata tccaggcact
      541 gaagcgattc attccgcgtt gcaatcgatc atcaaggatt gcttttggga cagtgctgtc
      601 tctgaggatg atatccgtac tattgttggc acggaccagg ttcgcctgaa gcagaaatta
      661 ttcgcaaaaa tccggtttca cgtacacggg cgctattcgg atgatctcga tttcttcgct
      721 ccggacgacc tgcgctttgg cgagaatatc aagcaggtgc tcgatgccct caccggtaat
      781 ggagtacgct tcgcacgagt catttgtctg gagcgaaatg ctggagattc tccaaaagaa
      841 agcgcctgtc gagaaggaac tcctcataga gcggatccgc gcctttcccc tggattggtt
      901 gtcgaacctg aaaatactgc aaccgcttac agtggataag gaatcaataa cgcgactctg
      961 tgacgatatc gctcgcggcg gcagtaactc attccatggg tccgattcag gtgtgcgcta
     1021 ctagtgtccg cgcgcttcgt tgtagagcgt cctaagaaaa tagcgtgctc ccattcatgg
     1081 atgggacatc cgtaaccggc caggcgtaac cgctcaccat cgctatcccc gcttctcgct
     1141 tcgcagccgc tcgataagct tcgcaagaaa aacccccgca tcgtccgtga ccacgtaatc
     1201 cgagagcgcc gcgatgggcg cgccggcatt ggtgttcacg gaaacgatga tctcggaatc
     1261 gacgattgac gcggtgtgct ggatctggcc ggagattccg aagccgagat agaggcgcgg
     1321 ccggatggtc ttgccggtct ggccgatcat gcgctcctcg ccgcaccagc ggttgaagac
     1381 gggcacgcgc gtcgcgccca cctcgccgcc caggagctcg gcgagttcaa agaggcgctc
     1441 gaagttcttt ttcgtcgcaa gcccgcggcc gccggttacg accaccggcg cttgttcgag
     1501 cggcaccttc ggcttgtccc atcggagcgg cgcctcgagg ctttcgatgc ggagcttcgt
     1561 caggtcatag gaaaacggca gcatctcgat gtccgtcgcg acatcgtccc gctcgcgccg
     1621 ctcgaagacg ccctgctgca cggtggccat ctgcggtcgt gcgtgcgggc agatgaccgt
     1681 cgcgatcgcg tttccgctgt aggccggtcg cttcatgacg agcaggcccg gtccgtagtc
     1741 ggggtggtcc atgatggaaa ggtcggtgca gtgcgaggtg aggccggtcg cgaatcgttg
     1801 cgcaagtcgc ggcgcgagat cgcatcccgc ctcggtcgag ggaaagagga cgatggacgg
     1861 ccgtcgttcg gcaatgaggt ccgcgaacat gtcggggaca atctggctgt cgtagtgctt
     1921 gagggccgga tggctgcagc agaggatggc gtccggtcca tgccgctcaa tggccggaag
     1981 gtactgctcc gcgtcgagcg cgaagacaag cgcgatgagc ctcgacccga gcgcgtccgc
     2041 gagttcccgg ccgcgcgtga ggagctcgag cgcgccctcg agcacgcgct cgtggtcctg
     2101 tatctcggcg aagacccaga cgtcgcggga atcggcgtat tgttcgtacg gagcggtcat
     2161 catcaacccg tcaatatcga agtatgttgt gcttcttgag ccggtgcaca agggcctcga
     2221 ccttctccgc ggtgccgccg ggaagcgcct cgcgtccgcg ggtgtgcgaa aaaagctcgg
     2281 cgccgattac gatcgtgggc gaccccgacg cgccatagtc ggattccgat cctccgatgt
     2341 cgtccatcgt catgcggcgt atgggctgaa cggcgccggc ggcgatgtcg gccagccggg
     2401 ggtgccgcac ccggttgtcg cgggagccga ccgacaggag cgccggcagc gggcagcgga
     2461 tcgtctggta ttcgtggccg tactggcgct cgatgatggc gtgcgcgccg tcgatccgga
     2521 tgcggtggat ctgggagacg agcggcgcgt cgagaaactc ggagagctga taggcgacat
     2581 ggccggtgtt gccgtcgatc gcctcgcggc cggtgacgat gatatcgaag gggccgagcc
     2641 gttcgatggc gcgcgcgagc gcgcggctgg tcgccagcga gtccgccccg gcgaaccgcg
     2701 gatcggagag gagaacgccg cggtcggcgc cgagcgcgca ggcctcgtag aggacctcgt
     2761 cggcatgggg cgggcccatg gtgagcgcgc acagtgttgc gccgtgcgcg tccttgagcg
     2821 cgagcgccat ctccagcgcg tggcggtcgt cggggttcat gatattaccg gtggccgagc
     2881 ggtcgatggc gcccgtcttc ggatcgaaga cggccttctg ggtgtcgggg acctgcttta
     2941 cgcagacgat gatcgagagc atgtccgtta catcctgccg aattccttca tgatggcgtt
     3001 gccgatgatg atattcatga cctccgaggt cccttcgtag atctcggtga tcttcgcatc
     3061 ccgaaagaag cgctccacgg gcaggtcctt cacgtagccg tagccgccga agacctgcag
     3121 cgcctcccgg cagacgcgca tcgccgcgtc ggaggcatag gccttgcaca tggccgcttc
     3181 cgtcgaaaag ggcaggccgc ggtccttgag gcaggcggcc cggcagacga gcaggcgcgc
     3241 cgcatcgagg cgcgtcttca tctcggcgag cttgaaggat accgcctgaa aggtgccgat
     3301 tggtttgccg aactgcgttc gttccgccgc gtaggtcgcc gcagccgcga aggccgcctc
     3361 gccgatgccc agcgcctgcg ccgcgatgcc aatgcggccg atatcgagcg tttgcatgga
     3421 gatccggaag ccctcgtccg gacggccgag gatgttgtct ttggggacgc gcacattgtt
     3481 gaagaagagc tcggagacca tgttgccgcg catgcccatc atgtcttcga tcttcccgac
     3541 ctggaagccc ggcatcgatt tttcgatgat gaagatggcg atgccgcgcg ccttcttcgc
     3601 gggatcgagc gtggccgcga cgaggaagag gtcgcccacg ccgccattgg tcacgaaggc
     3661 cttctggccg ttgatgatga aggaatcgcc gtcgggcgcg gcggtggtct ggatggaggc
     3721 ggcgtccgat cccgcctgcg gctcggtcac ggtaaagccg ccgatcttcc ggccgctcgc
     3781 gaggtcggga acgaagcgct cgatctggtc cggcgtgccg aacttcagga ggggcgcgag
     3841 gcagacggaa ttgtgcacgg tcaccccgag gccgacggcg gcgctcaccc gcgagatctc
     3901 ctcgatggcg atggcgtagc tcaccgtgtc gagctccgcg ccgccgtagc gcacgggcgc
     3961 ctggatgccc cagaggttga tgcggcccat ctccgcgacg atctccgccg ggagggggcg
     4021 cgattcgcgg tcgagcacgt tcgcccattc ctcgagcgtc tcgcgcgcga aggcgcggac
     4081 ggcgcgctgc accatgagct gggtcctgtc gagggccatg tccatggcgc gatcagaagg
     4141 gcgcgaggcc ggtgatgtca agcactcttt cccgcaggcg cgtcatgaag gccgccccgg
     4201 gatcgacctt taccgtgcgg tacgccggat cgcgctcgat ccagagcggg tgacgctcaa
     4261 agcgtcgata ctcgtaatgg ccgatgagat atgtaatggt gggaaagcgg ttcgcgagat
     4321 ggcgcaccag tcgcgcattt gccgcaagtt gcgcagcggt gagcggattg gccggaccgc
     4381 cgacgttctc gatcccgatc gcgcagtgat tgaggccgat cacgtgccgt cccatgagcg
     4441 tctccggcat gagacggtag accgtcccgt cgcggtccac caggaaatgc gccgatacgt
     4501 tcacgcgccc gccgcgcgcg atctccggtc gcgacggcgg caggatctcc ggcgcgaagg
     4561 tgtcgtatgc ctcctggagg gtccgggtcg ccgtccagtg caccacgatc atgcgggggg
     4621 tgatggcgat gtccgtcgtt gcgggatcgt agtgcaggcg cgcgtactcg agggtgagac
     4681 gacgccgcgt ctcgccgaac gggatcggcc ggtcgaggat gcgcacggtg tcgacatcgc
     4741 gggcgggagc ggtgtcggcg cagaggaggt gcgcgaccgc aatgagcgcg atgaggacgc
     4801 gcgggtatgt tcgtgccggt gtcatcatca gcacactacc ggaacggcac gacacggcaa
     4861 atgttttttt tcattgcgcg cagtgtgggg ggtgatgatg atgtatggcg cgaccgggca
     4921 tgtcgcggcg ggaggcgcct tcgggatgaa cggaacttcg ccacacggaa atcgatacgc
     4981 actgcgccat gcggcgcttt gtgccggtct gatggttctt ctctgtatcg cgagcggatg
     5041 cgataaggcc ggaaagcggg cgggagcgaa gaatgaaatt accgtcgcca cctataatat
     5101 ccgctattgc gcggcgggac gggggccggt gctcaatgcc attcgctccc tcggcgccga
     5161 cgtgatcgca ttgcaggagg acccggtgcg cggcggcgaa agcgccggat cagccttcgc
     5221 gcgcgcactc gggatgcacc atgcatcgag cgccccctat gccgtcgaga acgcgatgcc
     5281 gtgggcgctc tccatcctga gcgcgcaccc gatcatctcg cgagacgagc tccgcgtgaa
     5341 atcggggcgg cgcgccctgc gtgtacgaat ctccgtcggt ggcgcaggct gtgagctcgt
     5401 gaccacgcac ctctcgccct tcgtgtggga tgctaccaat cccgtcgccg ccaaccgggc
     5461 gcgctccctc aggcggcgcg aggagctgcg cgagctgcgg gcgtggctgg ggacgccctc
     5521 gaccccgcgc atcctcgtcg gggacttcaa cagcctgccc gtgatgggcg agctcgcggt
     5581 gctcgccgat gacggctatc ggtccgtctg ggacgcgctc gacatgccgg ggcaggggac
     5641 cttccggttg agcgcaagcg tccattcgtt cgtgaaaaag taccttccgg tcgacgagga
     5701 gatcatgctc gactatatct tcgtttccgt gggtatcacg gtcctgcagg ccggggtggt
     5761 gcgctcggat gcttcggacc actatccgct gacggcgcgc attcgcctcc catagggcga
     5821 ttcgggaaaa gcgtccgtgc gagtctgcga ttagatgggc gggcatatcg tcaccactag
     5881 gccgtgtcgc tgtgggatcg gcgtcgaaat tcagatcgca tcccctgccg tctcattcgg
     5941 tgcccctcag atgccgtaga ttccgcggaa agccgaacct gtttcgaacg taactaatgg
     6001 ttgaatagag gttgaataga ggttgaatag aggttgatcc ggatcatcag gcggcaggta
     6061 ggcggggcgc attcgccgaa tccatgggaa atatgcttgc atttcgtctt aatcggggtg
     6121 atgatgccct gtcgccgaaa ggatatccta tgccggaaaa ccgattaaag gtccctctcg
     6181 ccgtttcgca ggacctggtg aagcacatca ggctgctcgc cctcgccggc aggcgctcgt
     6241 tcgatcgcta tctcgtcggg ccgatccgcg aggcggggtg gcggcccatc acgagcgcgc
     6301 aaaagacccg gaagatgatc gagcgcatcg gcatcgagat ggaaaacccg gcgtaccggc
     6361 acaccatatc cccccgctgc aagcgtcttg tccaggtctc gctttccgag agcatggccg
     6421 ccgtcgggga cgcctcgatc ttttttctcg agtccatgca ggccgacgcc atcgtcgcca
     6481 gcggcgagga gtcgatcgag ttcgcggaca tcatcgagcc gccgctcgcc gagtttcgcg
     6541 aggtcaacag cacgaagagc gagcgcctct tccgggaaac catcgaggcc atcgacggcg
     6601 aagagctcaa gaccgccttc gatccggtcc ggctgggcaa tcccgagcag aaggtccaga
     6661 tggaccgcga ggtccattca ctctaccgcc agatcgtcgt cgccagcggg cgcaacgaca
     6721 tcccgaagtg ccgaaagctc atcgccggat acctcatccg gtccagcgac cgcgaggagt
     6781 acgacgcgca cgaggtggag aaggtcatcg acgcgctgga gcagcgcgag cccgggttcc
     6841 gtcgggacct gatggacatg ctcgaggccg atctgtacta ccgcatcacc cgcggcatca
     6901 tcgaggggga cgtcgcgaag gccatccagg gcatccgcaa gtattcctat gtcttccagg
     6961 ggtcgcccga cacccggcac tactacgacg tggaccgcct ggagcgcgcc ctctaccaga
     7021 tcatcaccga caagaacctg tgggacgact tgaaaaaatg aaataaccta tcatcacaag
     7081 gaaaaagaca atggctgaac caacactgac gttgaaggac atgacccagg gggccgtgag
     7141 cgtcctcgcg atcgccgggg agctggacgc gaaaacggcc ccgcagctca aggcgaaact
     7201 cgacgcgatt gtcgccgggg gcgtcgtcaa gatcgtgtgc gattgcgcgg ggctcaagta
     7261 catctcctcg gccgggatcg gcgtgctcaa tgcggccctg agcgcgtgca aggccaaggg
     7321 gggcgatatc gccctggcgg gcgtgtccaa gcagatcaag gacacgctgg acgtgatgta
     7381 cttcacgaag aaggtgcgcc tcttccagaa gatcgccgac gcggtgaacg agctgtaggg
     7441 cggcggggcc ggtggccggc gcgatcgaaa aacgggtgat cttcacgaac gacctggacg
     7501 agctctcgcg gatccgcgac gagatccagg acttcatcgg cgattgcctc gaatatgtgg
     7561 aacgcaaccg cgtgatcctc gcgatggacg aggcgatcgc caatatcatc aagtacggct
     7621 atccgaacgg cgggcgcggg accgtcgagc tggtcatgcg ccgcgacgat gtccgcctgg
     7681 agttcgtcct taccgacgac gccgtcccct tcaatccgct cgagcttccg gacgccgacc
     7741 tggagcacta tcacaatacc ggcgcctcgg gcgggctggg ggtgtatctc taccgtcggc
     7801 tcatggacgt gtcctatgaa ccgaccccga gcggcggaaa ccggctcacg ctttcgaggc
     7861 gaatcaacga gggggactca tgaaaaagaa atggtaccgc gcgctcgtgc cgggaatccg
     7921 gatcaagctg accctggtca cgacgctgtt cgtttcgctg gtgatcgccg tcacgtcgtt
     7981 tttccactac cgacagcagc ggcagacgct cgaggaggcc atggagcgcg aggcggcggc
     8041 cccgctgcgc ttcgtcacgg ccctggcggt cgagcgcgag aacctgaagc ggggcatcgt
     8101 ctccctcgag gagctgcgac tgcgcatcca ggaaaagcgg aaggagctgg gccgatatcg
     8161 cgtcgcggtc tatcggcgaa agagcacctt cggcaatacc atgcgccggt tcgggagctg
     8221 gctggggatg aaggtggcct acgactatca acaccgctcg gtcgatacgc attacagccg
     8281 ctatctctcc gacaaggaca tcaccgtgct cgaggagcgg atcaaggcgc agcttctcgc
     8341 gggcggatcg cgtgcgcccg ccgacgacct cgcgaaactc aaggcggcgg cccgtccggt
     8401 cgccgtatcg ctcctggcga tcgccgcgct cgaggcccag gcggagtcgg ccgccgatgc
     8461 cgaggcgaaa cggttcgcgc ggctcatgga gggcgaggag aagaaacggg cggcggcgga
     8521 gcgatcactc gattcgacgc tcaacgcgac gctgggccgg gggtatcaat cgcgcgccgc
     8581 agagcaggga ctcaccggcg acagcgtgcg gatccagtcg atcaatcccg gcggagaggt
     8641 gtacttcgat acgggaagcg tcttcggcag gaccacgtat cggttcgacc ggctgttccg
     8701 aaacgatgcc tatgcggccg gacgtaagtc attcatcgcc gaggccgatc ccgtctccct
     8761 ccttgccgac ggtccccgcc gtcaaagcta tcgggtggtc gacagccatt tcgaggtgac
     8821 ctaccgcccg gtgttcaaga atccgttcat cgaggagatg gcccgcgcga tgctcgtcga
     8881 gctgcggacc aaccgcgccg cgtggaagcg ctttctcgac gacgatcggc ggatcagcga
     8941 gtcgatcgcg caggtcgcgg aaaagctcac ggcgcgcgtg caggcgctca ggaaaacgcc
     9001 cctcgtgaag cccgccgcgg accgcgagta ccggtcgctg taccgggact atcaggcgct
     9061 ggtgaaacaa cgggcgcagt cctttgccgc gaacaatccc catgcgaaga cgcacgatca
     9121 gcgcgccgcg gccatcgccg ccgagcggac cgcgctcgat gcccgggaca aggagctccg
     9181 ggccgcggtc ggcgccttta cgccggggca aaccgtccgg gtcgcccctg acgggatcga
     9241 ggtgcgcctg gaggcgctcc tcgccgagcg cgaccgcctg aagcggcaat ccgccgagct
     9301 cgcggccagg cgtgacgatc ttcgcgattc gcccgagcgg tttgccgatg cgatccggca
     9361 tgtgcgcgac gcggtcgtgt tctcatatgt gatgcttccc tatcgcgtcg atccgttgca
     9421 gcacgagcgc tatctggccg gaaaggcctc gcgcgacgcc ctccagctcc gctgggcggc
     9481 cctccgcgca tggatcgcgg agggggcgtc ggagacggac acgcccagcg tcgtcgtcgg
     9541 cggcaggcgg gagccgctta tggccgacgg cgttctcgcg cgcagccgca gcgaggcgga
     9601 ggagttcctc tggaagatcg actcgctgcc gcttgccgat gacgagggcg atcacgacgg
     9661 cgggatcatc gcgctcatcg tggcggacgg gcacatcggc tacaacgcca tcttcaccga
     9721 caagaccgac gggatgcgcg ccatcatcga caaccgcaac gagatcctcc gctacggcgt
     9781 gacgatcgcg ctggtcgcgg tcgtggcgat ctttctcctc tcgagcctga tggtgcgccg
     9841 catccggcgc atcatcgcca gcgcggagct ggtgggcgag ggcgacctcg aggtgcagtt
     9901 cccccgcgcc gggagcgacg agatcggcac gctcagcgag tcgctggacg agatggtgaa
     9961 ggggctgcgc gatcgcgagg agatgcgcgg ggagatggtc gccgccgagg agatccagcg
    10021 ccgcctcctg ccggaggcca tgccggcggt gatgcaggag cgcctgttgt gccgcacctt
    10081 ttacaaggcg atgcggggcg cgggcgggga ctatttcgat ttcatcgatt caggggagga
    10141 cgatttctat ttttgcatcg gcgacgtgtc gagccatggg gccggcccgg ccatcgtgat
    10201 ggcgctcctg cgcgcgcagc ttcgcgggat catcaggcgg ggcggccgcg atatgaagaa
    10261 gatcctcacg gagatcaacg aaagcgtcta tgccgatacc ccgccgcata ttttcgtcac
    10321 gttcgttctg ctccgctgca ccgtcagcac cggcgagatc gcgtattgtt cggcggggca
    10381 ttgcccggga ctggtctatc gggcgaactc cacgacggtc gagacgctcg aggggcgcgg
    10441 gctccccgtc gggatggacg agagcgagct cttcgagacg accatcaaga accggtcctt
    10501 ccggctcgat cccggcgata tgctctttct ctatacggac gggctcaccg aggcgatgga
    10561 cccgcatcgc gaactcttcg gggaggagcg actccagggg atcatcgcgg aacagggcgg
    10621 ggcgacgccg tccgagctcg tcgacgttct ggtggccgcg gtcgagcggc ataccggcac
    10681 cgaactgaag ggagaggggc cgtcgcagct caatgacgat atcgcgatga tcgccatcaa
    10741 acgaacatag gacacggacg ataccatgaa ccagatcacc atcgacgaaa cgaagtgcaa
    10801 gcacgacggc gcctgtgtgg ccgagtgccc cgtcggcatt atcatgatgg ccgacaagaa
    10861 atcgcttccg aagatcatac cgggcgggga ggaattctgt atctcctgcg gacattgcgt
    10921 ggcggtatgt ccgcacgggg cgcttgcaca cacgatcgcc cgtccggaag atctggcgga
    10981 ggttaagaaa gaatggatgc tttccccgga gcacgccgag cattttctgc gctcccggcg
    11041 gtccatcagg gcctacaagg acaaggcggt ggaccgtgaa acgctcgagc ggctcatcgg
    11101 cgtcgcgcgc cacgccccca gcgggcacaa tgtgcagccg gtgcagtgga tggtcgtcag
    11161 cggggcggag aagatacgcg agctgtccgg cgcggtcatc gactggatgc gctacatgat
    11221 caaggaaaag ccggagatcg ccacgcccct gcacatggac ggtgtcgttc tctactggga
    11281 gatggggcgc gacatgattt gccgtaatgc gccgcacata ctcgtcgcgc atgcccccag
    11341 ggcgctcccg caggcggcga cggacggcac catcgcgctt gcctatctcg agctcgccgc
    11401 gccgtcgctt ggtctgggga cctgctgggc gggctacttc catgtcgcct cgctctattg
    11461 gccgggcatg aaaacggcgc tgggactccc cgacggccat gtgagcctgg gggcgatgat
    11521 ggtgggctat ccgaaatacg ggttccatcg gctgccggag cggaatgcgc cacaaataat
    11581 ctggcggtag gacgtccgcg cttcgcgcgt atcaatccga tcaagcctcg ccgtgctgat
    11641 tcgatttctg ctcaacgggg tgcagcagaa gcgaaaggat cgctccgacg agcgacgcgc
    11701 ctcctgcgat cgtgaaggcc agcgtgtaac tcccgaacga atcggcgacg cgcccggcca
    11761 tgaagggtcc gagggcctgg ccgatgccga aaaagagcgt aatgaaaccg agagcggccg
    11821 atcccatgcg cggcccgaga atgtccccgg aggccgccgc catgatggcg ggaatgctcc
    11881 aggcggtgac ggcgaacagc agggctgacg catagatgcc gggtgccgat ttccacaatc
    11941 cgaacaatac gtagcacgcg aactggatgg cgtagacgca ggacaggccg tacttccgcc
    12001 ccatgcggtc tgagaccgtt ccccagatga acccgctggc gatgctgatc ccgccgaccg
    12061 ccgaccagag cgcgcccgca tcggcgaccg tgaagccgca ttcccagttg agatagcgca
    12121 cgaagaatgt cgcgtagatg atgtacgaaa aaccgtaaag gctgtagatc gcggccaagt
    12181 gccatacggt cggcgttcgg tatacgagcc gcatcgagtc gacgagcgag atcgccgcac
    12241 tatccgtcgg gggtgcggat ccatcgtgtc cgatgggcga gagtcccagt tccgatggcg
    12301 tgttcctgag gacgaacgcg cacaccacgg cgatcgcaac cgcgatgccc gatagagcga
    12361 accatgcgta gcgccaaccg tccacgccgt aggcggcgag tattttcgga atgacgatgc
    12421 ccgcgaacag gagcgccgcg ctcgatccgc tcacggtgat gccggtcgcc agcccgcgtc
    12481 gcgacacccc gaaccacgcg atcacgatgg caagcacggg aatgttggtg ccgccgcttc
    12541 ccatgccggt gagcacccgg ccgatgagcg cggtgaagaa gtccggcgcg agccccgtca
    12601 ggagcatggc gccggccagg agcgccatga aggaggcgat gatcttccgg gggctgaagt
    12661 gcgccgagag gaatccgcag gtgagcgcaa gcagcaggta tcccaccatg ttccccgtcg
    12721 caaggtcgcc ggcctgcgcg tcggtgaggc ccaggccggt cttcatggcg gggaagatca
    12781 tcgtgtagcc gaaacggccg aagccgagcg aactgaacac cgtgaggacc gacgcaccga
    12841 gtattatcca gccgtaatgc atggcgggcg gctagcggcg tatcccaagc gaggcgacgt
    12901 attccgtgtc gcttttcagg atgtggtcga tgagccagtc gcgaagatag ctcgtcacgt
    12961 cctggaagag ccccatcttc tttttgctct gccgtgcgat gaaggtgtcg atccggtcga
    13021 tgaactcgcg gtgcaggtgc tggtgcgcct cgagccccgg atagccggcg tgcgccaaaa
    13081 gttcctcctc ggctctgaag tggtattcgg tatagcgctt gagttcggca agcgccgcgg
    13141 cgagcgcctc ggtgcgcgaa ccgtccatga ccgcctcgtg gagggcgttg atgaggtcga
    13201 agagcctctg gtgctggctg tcgatggtcg ggtcgttgag gctgaacgaa tcgttccaca
    13261 tgatatacgg catacggtac cttcgtactg gaatgatccg ctcagggtat gtcgaacggg
    13321 tgcgccctgc aattaaaaaa tagcgctaaa acaaaaaaca agcgttgaca agatttgaac
    13381 gtaatcgctg aaatgccgac atgagaaaaa ccgccatcgt ccttgcagca gtatgtttga
    13441 tcgccctctg cggcgccatt gcggcctgtc gcggcgcgga tacgcgcgcc aagccggccg
    13501 cctcgaacga accgcgctat cgcggcctgt acatatcgaa caaatattcc gtgaacctgc
    13561 cgttcatgaa gcgccttgtg gagcgcggga aaccgctggg cgtcaacatg ctggtgatcg
    13621 acgtgcaccc gtacaagatc ctccggccgc gtatcaacaa ggaggtcatc gactatctca
    13681 aatccgagaa cctgtacctc gtggcgcgcg tcgtgtgctt ccaggacggg ctggacaagc
    13741 tcccggtgcc cgaaccgtac atggagcgcc tgatgaacgt cttcaatacg gcgctcacat
    13801 acgatttccc cgaaatccaa ctcgactaca tccgcttcaa ggacggcggg gttccctatt
    13861 cgctgaagag gaagtacgac tacatcgccg atctcctgca gtccttcagg accatatcga
    13921 acgcacgcgg cgtgaaactc tcggcggacc tcttcgggcg ggtcatgtac aataaaaacg
    13981 accccatcgg tcagcagctc gagctctttg cgcgctatac cgacgtcatc tatcccatgc
    14041 tctaccccag ccacttcacc ggcgaccggc agcgtctctc caatccgggc gcgacgatca
    14101 aagaggggac catgctgggc atcaatcgcc tcagcgggac cccggtgcgc gtccttccct
    14161 acgtgcaggc attcggctac aatatcggat gggcgcgcgt caatctggtc aagtacgttg
    14221 agctgcaggt gatcgcggtc gaggagacgc cggcgggcgg ctgggtcgcc tggaacgcga
    14281 agggcgacta cgagagcgtt tttcgtgcgc tcgacaacct gaaagcgccc gtcgcctctc
    14341 aggtggaaac gcggtaaacg cgcgcgtcga tccgccgccg gaacgcgcgg cgcgcggcct
    14401 gcggtgcggt cgggtcggat tatacgggac cactatgcca gtatacgaat ttacctgtga
    14461 ccagtgcggg atcgtcttca gcgagcttcg ccgaatgggc gatttcgatc cgccggcctg
    14521 cccggaatgc ggctcgcgtg acaccagaaa gatattctcg atgttcgccg gcgcgggttc
    14581 cgccgcgtcg tgcggcacat gcaccaccaa gccgtccggc gggtgaaagg cctgccattg
    14641 aggccggtcg gcctcatcac caccgcttct cgggagccat catgaaaaca gccccgctca
    14701 ttctcgttgc cggcggtacg ggcagcggaa agacccatct gatcgatcgc atgctcgccg
    14761 cgaatccggg cctgcgctgc gcggcgctcg tcgattcccc cggtaacgcc gcgatcgatt
    14821 gcgccgcgat cggccgcggc gtcgtcgtcc cgctcatgca gggcgcgatc atcctgcgga
    14881 gcttcccgcg cgacctggac cgtgtggtca cgcgatatgc gccggacgcg attgtcgtgg
    14941 agcttcgcgc gggaatcgac ctgtcgggac tgctgggccg attgtaccgc acccgggccg
    15001 tgctgatggg cgtcatagcg gtcatcgctc cgtccggcgc cgcgctggat gaaaacgatg
    15061 cggccgcgct tccgcttgcg gacctcgtcg tcgtcaacga cggcgcgccg ggggccgggg
    15121 acccgggcgg gatcgtcgcg cgggcgcgcg agatcaaccc ccgtgcggaa atcctttccg
    15181 gcggttcgct cccttcaatg gatagtatga tgaacggacc gcgctacgat atcgccgcgc
    15241 ggctgcgggc atgcgggtgc gacggtccga cgggcggggc cgcgcttcgc gatgattccg
    15301 gcagccacgc gatcgatttc ttcgacggcc gttgggtcga aatcgatgcg cttcgatccc
    15361 tgctggcatc gccgccggta ccgcttgaga ccgcgaaggg attcgtctgc cttcagccgg
    15421 acggcaggcg ctaccgcttt tatcaccgga acgacacctt tatccttcag gaggcggctc
    15481 cgtttcccga tgcgcaggtc gaaacggccg attggatgca tcgccacgat cacgaccatg
    15541 acgactgtga ttgcgaatcg atggagacgg atgagggttc ggactcgttg gcggaattta
    15601 gcgcgatcac cttcacggtg ggatgcgatg atgcgggtgt cgtcgacgcc gagctcgcaa
    15661 tggcacgggc catggagcgc tgctgggagt atccggacgc cggtccgcgc gccggcgcgc
    15721 ccgacgacgg cacgatcgag acgatcgcga accttatgca cgacgagaaa ttcatcgagg
    15781 cggcctcgct cgttcaggaa tgcctgcggt cgtgcgcgga gatcccgtcg agcctgtacg
    15841 gctttctggc cgagatctac gatgcattgg gaatgccgta tcgggcgctt acgctggcgc
    15901 gctgtgcctg catagcggga agcccctccg tcgagacgct cgagcgcctg gcgcgggcct
    15961 ataacgccgt cgatgattgc gcgcgggccg tgacggtcct cgaccgctgt ctttcgctcg
    16021 atccagagtt tatttcgggg ctgctcaatc aggcgcggct caacttcgcc tacgaccggc
    16081 tcgaccgggc gcgcaacgcc ctcgagcgcg cccgcgccat ggacccggac aatccagacg
    16141 cgatcatgct cgcgggccag atcgccgacg aatcgggcga tcccggcggc gcccgtgaag
    16201 agttcgagcg actcgccgcg gcggccccgg atgacgcgga gatcgccttc cgtctcgcct
    16261 ccgcctgcaa cgattgtgac gatctcgatg cggcgctgca tgcccagcga cgcgccgctg
    16321 aggcggatcc ggacgacgcc tatgcgcggt attgctgcgg ctttatcgcg ctccgtgcgg
    16381 gcgacgatgc cgacgcgctc gaatggctcg aacaggcgcg cgaggggggc ttcacgaaca
    16441 gccggctctt cctgtgcgcg ggtatcgcgc atacccgcct cgatgagccc gacgacgccg
    16501 cgctgcggtt cgccgatgcg cgcgatgccg ccatggacgc cctcaatgac gatcccttcg
    16561 tcgattccct catcgaacag gcgatcctcg cgcacatcgc cgtgggggaa accgatgatg
    16621 cccggcggcg cgccgcattg ctcgccgtcg atcccgcgtt cgccttctcc cgggagttcg
    16681 gccagttgtg ccgaatgatg gatacctttc ccctggcaca cgagcttctg cgcatgtccg
    16741 aaagctagcg cgatcggata acgcccatgc gaccggagtt tcgcgaaata atccgcatcc
    16801 tgctcacgcg aaagtcgata ctcgcggcga ccatatcgat cgccctgatc gaatcccttc
    16861 tggcgatcgc gctcccatat ctctcccgtg tgcagataga ggtgctggaa tcgaggatcc
    16921 tcgcgccggg cgccgcgctt tcgacgccgc tggcgctgtt cgtcgcgctc ctcgccgcga
    16981 tctcgctcat ccggcttctc caggcggccg ccgccggtct ggggcgggtg atcgacgtat
    17041 acgcgcgcga gcgctttacc ctccaggccg atgcgctcct ctacggaaag atggagacga
    17101 tggacgcggg tttcttcaac aacccgaaga accgccgcct gctctacgtg ctcttcgacg
    17161 tggggcagtt gccgggcgcc gtcctccagt tctgcaaatc gcagggacgc gtcacggtgg
    17221 cgctcgcggg gatcttcccc atcctggccg tcgtcgatgc gcgcattctc gccgtcatcg
    17281 ccgcggccgg cgcgctccaa ctcctggttc ttcggtttcg catccgggcg gagaattcat
    17341 atcggctcta caaggagcgc cggctcgccg acatcctcga gatgaagttc ctgcttcgct
    17401 atcactatca ccaggtgctg ggggtctcgg gcgaggaccg catcatgccg cgctactggt
    17461 cgcgcgcggg ggagctggtc gcgctggaga cggggcagga gcggcttgcc tttcgctatc
    17521 agctcgtgaa tgtattcgtg gagcaggggg cgcttgcggc ctcggccgcc gttgccgggg
    17581 caatggcgct ctccggagcg ctttccatcg gggcgtttac catggtcacg atgtatgtcg
    17641 tctatcttca gtcctgcttc ggcgacatca atcagagcat caatgaattc taccggctgc
    17701 gctccgtgtt ccttcagctc ggcttctttc tggcgctgcg cccgcgcgtt gcggtcccgg
    17761 atggcgcgcc ggcgaaggcc gccatcgccg gcgacatcga ggtgcgcgat gtctcgttcc
    17821 ggtatccggt cctcacgacg gaggaatgcg attatctgtc gcatctggtc acgcgcctca
    17881 atctcgcgaa caagcggcgc gaggtatacg ggagcgacct gtcgatcgtg cgcgaatggc
    17941 agcggctgct cgacgagaac catgcgccga accccctcgt gctcgacggg gtttcctgcc
    18001 gcttcgcaaa cggatcgatc acggcgctgg tgggccgcaa tggatcgggg aaaacgacgc
    18061 tcatgaacct gctcatgcgg gcctacgatc cggagcgtgg cgcgatcgcg atcggcggcg
    18121 ttccgcttgc gacggcgcac ccggcggccg ctcgtcacgc catcagcatg gtcgcgcagg
    18181 cgccgttcct gctggagtca tattcgctgc gcgacaacct gcttctgggg gccgcgcgcg
    18241 agtacgatga cgcggcgctg tgggacatcc tcggcgagct ggggctcgcg gacgaattgc
    18301 ggcgttttcc cggcggtctc gatagcgtcg tgggcgacga ggtgaacctc tcgggcgggc
    18361 aggcgcagct cctgtgcatc gcgcgggtga tggcgcagcg gcggcgggtc atcatcctgg
    18421 acgaggggac caatcagctc gacgcggaga acgagctgcg gatcgtcggg atccttgagc
    18481 gcctgaagcg cgacgccgcg gtcatcatca tcacgcatcg catgacgacg gcgcggaagg
    18541 ccgacctgat ccatgtgatg gacggcgggc gcatcgtcga gagcgggacg catgttgagc
    18601 ttgcgtcgcg ggacggcggg gtgtatcgtg gattctggga gattcaggtg gtccgctaaa
    18661 aaacaaatgg cgccatgttt ccatggcgcc attggatctt cccaacgaac aaaacgctac
    18721 ttcatgacct tggtgcggtg gcactggcag cagtaccctt cgcgtttgtc agcgcggcac
    18781 ttgtcgcact gcttgtggcc acagtatgag catttcatag agagtctcct tttgatgaaa
    18841 ttattggcat aaatatacag cgcggcaggc gcatcgtcaa tcatattttg cgaaattatc
    18901 acttcgtcaa cgttgttatt tgttcataaa aattctaacg aaagccatcg ccaggaagta
    18961 aagggcggtc cccaatgaga cgattgtggc gccggtcggc gtgtcgagga caaaggaaag
    19021 ggcgaggccg ccggtcatga tcgcaaaggt gtaaaggatc ccccaggcga tcaccagccg
    19081 gaagtttttt gagagcaaca gcccgaagct cgccggaagg accaccagcg cggatatgag
    19141 gatgatgccc actaccttga taccggtgat gatgacggcg gccatgagcg ccaggagcag
    19201 cgtgtcgagc gcgccggtcc gcacgccgct cgtcgatgcg acgtcctcgt cgaatgacat
    19261 gaacagcaag cggtgcagat agagccccac gaagaggtac aacaatacca gcgcaatcgc
    19321 cgccatgacc gtatcgaatg tcgtcacccc gagaatgttc ccgaagagat agcccatcag
    19381 gtcgaaggaa taggccttgc ggaccgcgat gaggatggcg cccagggcca tcgaaaaggc
    19441 gaagaagatg ccgatccccg catcgtagga aagaccgtca cggctgacca gccgccccag
    19501 ggtcacggcc gcgaggacgc agaaggcgag cgaggcgaaa aacgggtcga tgcccagaaa
    19561 aagccccaac gccacgccgc cgaaggccgt atgcgcaacg cccgcgccga gaaacgacat
    19621 gcggcgcagc acgacaaaga acgagagcat gccgaacaga aggctcatga tgacgcacat
    19681 ggcgatggcg ttcacgaaga agggcatgga gatgatctca cgcatggtcg tcccggcagg
    19741 tcgggcaatt cttgtcgtgg tgcagcacgt agacgttttt cccgaagacc tcggcgaggc
    19801 ggtccgagcc gaggcaatct gcgggcttgc cgtgaaaatg gagcttgcgt ttgatgcagg
    19861 cgacggtgtc cacgatcatc gatacggtcc cgatgtcgtg cgagacgatc acgatcgcca
    19921 gaccgcgctc atcgcgcagt ttccgcagca ggagatagaa cgagtcctgc gcgacggcat
    19981 caaggccggt cgagggctcg tcgaggagca ggatctcggg attaagcgca agggcccgcg
    20041 cgatgagcac ccgttgcttc tgccctcccg agaggctccc gaaatgggcg cgcgccgcat
    20101 ccgccatccc caccgtatcg agcgcgccct cgatggcctg gcggtccgtg cgggtgagcc
    20161 gttcgatcac ggccgtccgc gcaaagcggg acatggcgac cacgtcgtag gccgtgaccg
    20221 gaaagcgctc gtcctgacgg cgctcctggg gaagatagcc gatccgccct gtgcgcatga
    20281 tctcggcggg cgcggtttcg aggatggaga ccgtgccccg tgtcgggcgg gtgaggccca
    20341 tcatcgtgcg caggagcgtg gttttgcccg agccgttggg gccgacgatg gacacgatct
    20401 cgcgcggacg gacggcaagc gtgatgtcct cgagcacgat aatggatccg aactcgaccc
    20461 agacattgtt cagtgcgatg atggggcgtt ccatggtcgc cggtggccct tagcgggaga
    20521 gcgccgcggc cagcgcggtc gcattgtgcc gcatgaggga aagataggtg gcgcgtgatg
    20581 gcgtggcggg atcgccgatc gcatcaaggc gtatgatggg cacgctcagg gcctcggcga
    20641 tcgtccgcgc ctcggcgctc tgcgcgttca ggtcgatgat gacggcgcgg gcggacatgc
    20701 ggcgccccgc ggcgatgagg tcgccgaagg ctcgcagcga cggcttgtgc ccgtgtccgg
    20761 actcgatggt cccggcgatg acgagcccgt attcggcggc gaaataatcc cacgacgcat
    20821 gccactggat gaacgatttt ccgcggaccg gcttgagcgc cgcttcgatg tcgcggtgca
    20881 gatcgtcgat acgggcgcgg taggccgcga ggtttttttc gtacgcgggg cggttcgccg
    20941 gatcgatcgc acaatacgcg gcggcgatgc cgtcggcgat ggcgcgtgcg ccgcgcacgg
    21001 aaagccagat gtgcggattg gcggtgtcgt cattgaggag atagaccgtc ttcgcggttg
    21061 tcgggacaaa gcgggccgcc cacccgtcaa agccggagga aattccgatg tacaggcgcg
    21121 cattctgcag cgcgcggacc gccgacggcg gcggctcgta gatgtgcgga ttggcgcccg
    21181 ctgggacgat agcggaaacc gtcagacgat cgccggcgat agtgcgggcg atatcgtaga
    21241 ccggaaaaac ggaaacggct atctccggct tctccgcggt tggtccggac gctccgcagg
    21301 caagcatgac ccagatgaga agcgggaaaa acgcgcgatg cggcgcatga tcgcggctgt
    21361 ggattcgttt cgtgggtgtc atcatcattg tcggtggccg tcgttgcgag attgggatgc
    21421 ggccggtccg ccgtgtcaag ctaaaaagcc gatgtggggg atgatggcga ttggcgcgcc
    21481 agcgcgatca tacggcgaat ggattcgaag ccgatcgcgt cgaggtcgat atcgtccggg
    21541 cgcatgaaga cgatggccgc ggcgtcgtcg tgagcgcgca tgcgtgaaaa atccgtggcc
    21601 gcgcagataa atcccatgtc cagcgtgaag taggtgatcc cgccatagag ataacggttc
    21661 gggaacgagg cgaggtaggt cgccgtcgtg atctcgatgt tcagttcctc gcgcagttcg
    21721 cggcggaggg cctcctcggc ggtttccatg atgtccgcga aaccgcccgg aagatcgagc
    21781 atgcccttgc ggggttcgtt ttttcgttcg accagaagca ggcggcgcga atcgtcgaag
    21841 acgagaccgg cgaccgcggc ggccgcgttg acgaagtaca cgaagtcgca gcgttcgcat
    21901 cggaaggagc tggcatcgcg aagggcgaat ccccccgcac cgcagcgcgg gcagtgcttg
    21961 aagattctgc cgggatagtg ttcttcgatc gtaatcatct catgcgttcc gggtgcgctg
    22021 tgcatacgcc ttcatcacgg cgcatgtcat ggaatccgca tgccgacacg agcgtcaagg
    22081 acattaccgt ggacgcggac tggcggtagc ataaaatcat tgacgcgccc ctgcgggcgt
    22141 gctgtattct gtctcggtac gcatcatcga cacaggggag gggcgaatgg atatgggagg
    22201 aaaactcatc tgcaaggcct gccggctgat tatcgcggag agcaagcttg gcgcggtatg
    22261 ccctgcctgc ggcgtcccgc ggagcgcgtt cgaggcgtat gccgatccgg tgaagccgcg
    22321 gaggcggttt ttcatcgatc taaacctaca ccccatctca ctgcacttcc cgcaggcatt
    22381 atcgctcctg gtgccgtttt gcctcggtgc gggattcatt gccggcgggc agtggggggc
    22441 gcggttcgac ggcgcggcgc ttattctgtc gatgctcttc ccgctctccg ttctgggcgc
    22501 ctttacgacc ggcgtcttcg acggatcgat gcggttcaag aagttgcgcg ccccctatct
    22561 cgtcaggaag atctttctcg gggcgtcgct gttcatgctt tcagtcgccg ctgccgcgct
    22621 tgcgttcatg gtcggcgtcg agggcgcccg ggtgtccatc ggcgccgtat gcatggccgc
    22681 ggtactcgtc gagatcgcac tggcgcagat tggcaaatcc ctgatgtgcg ctgcgcttcc
    22741 gggataggtc atcggtatgc aaaaaacgca acgacggatc cgctgatctt tgcttgacgc
    22801 ctcgcgggta tttggatata ctgatggtgg aaggaaataa aacaggtgga tcacatacaa
    22861 acacatgaac gatcatagcg ccgagcgtcc ggaagcgggc ggcggcgctt ctcgcaccga
    22921 agacacgtca tttgaactcg cccatatccg gtatcaggag ctcctgcaga gtatctgcga
    22981 gggatactac gagtgcgatc tctccggtcg cgtgaccttt tgcaatgact ccttcgcgcg
    23041 aatggtcggc ctgcccgtcg agcggcttgt gggcgcgccc tatcggacct ttattgaacc
    23101 ggacgacatc ggcacggtgg agcctctttt tctgaggatc ctgaaaagcg gcgaaacgga
    23161 acgcttctcc gactggcgaa tgcgctccca gagcggcgcg cgcgtttacg tcgaggggat
    23221 ggcctcgctg atccgcgacg cacggggagc gccgattggc ttcaggggca tcgtgcgcga
    23281 catctccacc ttcatggccg tgcacgagga cctggttcgc agcgaacagc gctttttcga
    23341 tttcctggag tcgctccccg agatcgtgtt cgaaatggac atgaagggga actttttgta
    23401 tgtgaaccgc cgcggttgcg aggtcatggg ctattcgcgc gccgagatca tgtgcatgaa
    23461 cgccctggat atcctgcgcc tggacgagcg cgagcgggcc tcgcgcggca tcatcaaaac
    23521 catgctgggc aagcgctatt ccgcgatcga atacaccttc caccggaagg acggctcctg
    23581 gctgccggtg cacgtgtatg cccgccaggt ggtggagggc ggggaggtga tcggcatacg
    23641 cgggatcgcc atcgacgcga ctgagctcaa ggaggcggag cgcgcgatcc ttgaaagcga
    23701 ggagcgcttc cgcacgatga tcgagcacag caccgagatg atttcaatcc tcgactggca
    23761 tggggtgatc cgctaccaga gccagtcgac gcggcgcatt ctcgggtatt cgccggagga
    23821 gctcgtggga cgccgcttcg tcgatctcgt ccatgacgac gacgcgcacc aggtcgtcgt
    23881 caccctcacc ggcagcctca agcggatgaa tgaggagatc gtcgccgagt accgttttcg
    23941 cgacagggag gggcactatc gcgtacttga atcgaccggc gtgaacctca agaacagcct
    24001 catcaacggc atcctgctca atacccgcga catcaccgac cggaaggcgg ccgagtatgc
    24061 gctgcgaaaa cgggaggagc gcttccggtc gatgtacaac aacgccatca tcggactgat
    24121 gacgatcgat cgggcgagca cctcggtgct cactaccaat gagataggct tccgaatctt
    24181 cgggtgcgaa tcgaacaatg agtttctggg cgtttcgctc gggccgcgat tcgtggattc
    24241 ggacgcgttc gcccaattcg aacatgaggt catggagcac ggggtcgtga acaaccacga
    24301 aatcgagata cggcgaaccg acgggagctc gctttggacc gagatgtccg gcaagcacct
    24361 caccgaggcc gataatatcg agctggccat catcgacatc tccaagcgca agaaagcgga
    24421 ggagcaggtc gcgtattttt ccttctatga ccggctcacc gggctgccga acagggagct
    24481 catctccaat cgcatccaga ccgagatgct caaggcgcgc cgcaagggat cgcatgtctt
    24541 tttcgtgctc tgcatcggta tcgaccggtt caaattcgtg aacgatatgt cggggaccgc
    24601 tgcgggcgac caggtcatca tcaaggccgc cgggcgcctg aagatgagtt tcggcggcga
    24661 ggatctcgtc gcgcgcctcg acggcgacaa gttcgtcgcc gtctactccg acgcggagga
    24721 caccggcgac gtgctcgccg cggtacagaa ggccatgacc gcgttcgcgg accccttcgt
    24781 gatagacggc tccatcttcc cgatcacggc cagcatcggg gtgagcgtat atccggtgga
    24841 cgggacggcg ccgaacacgc tcatcaagaa cagcgaatcg gcgatgtacc tcgcgaagga
    24901 gcaggggcgc aacggttatc gcctgttcga tgtgcagctc aatgcgcaga tgcgccagcg
    24961 tctccagctg gaggccgagc tgcagcgcgc catctaccag aacgagttcg tcgcgtttta
    25021 tcagcccaag gtgcttcaga acggccgcat catgggcatg gagtcgctca tccgctggca
    25081 gtcccccacg cggggcctcg tgccgccgat gcagttcatt cccatcgtcg aatcgaacgg
    25141 catgatccgc gatatcggct ccctgatcct gctccagtcc tgccgccaaa accgcctgtg
    25201 gcaggacgcc ggccatccgc cggtcaaggt cgcggtgaac ctctcgccca tccagttccg
    25261 ccagcccgat ctgatcgaca gcatcgcgcg tatcgtcaag gacaccgcgc tggatccgcg
    25321 ctggctcgag ctcgagatca ccgagagcgg gatcatggaa aacgagaagg agaacatcga
    25381 gcgtctcagc gagatccacc gcatgggcat ctccatctcg attgacgatt tcgggaccgg
    25441 atattcctcg ctcagcaagc tccgggacta tccgatcgac acgctcaaga tcgacaagtc
    25501 gttcatcgaa aagcttcccg cggacgacaa gtcggcgacc atcgccacta cgatcatcga
    25561 cctggcgcac aatctcggct tcatggtggt cgccgaaggg gtcgagtcct ccgatcagct
    25621 cgccttcctg gacgatcgac gatgcgatca ataccagggg tatttcttca gcaagccgct
    25681 ccctcccgac gagttcgaga aaaaactgcg cgcgtcatag ctttgttctt gaaaaataag
    25741 gttcggtgtg cgcatgtgct gaaattaatc ctactcacag cgcaggtatg agctcatgac
    25801 ctcccggaaa agaaccacgg cgaacctcga ctggaaaaat cttccattcg ggtacgtgaa
    25861 aacgaacgcg aacattcgat actactggcg cgacggcgtc tggtccgagg gcgaggtggt
    25921 ctcggacgag aacatcagca tcagtgtcgc cgcgccctgc atccactacg ggcagcaggc
    25981 gttcgaaggg ctcaaggtgt tcgagacgaa gtcgggggag atcgttgctt ttcgacccga
    26041 agaaaacgcg atgcgcctca accgcagttg cgagcggatc ttcatgcccg cgattcccga
    26101 aaagctgttt gtcgatgcga tccatcgcgt tgtcgcggcg aacgtcgaat tcgtgccccc
    26161 ctacggcacc ggggcgagcc tgtacgtgcg gccgctgatc atcggggtgg ggccccgcgt
    26221 gggactgggc ccagccgagg agtatatttt cattgtcctg gtgagcccgg tggggccgta
    26281 ttacaaaggc ggcttcaagc cggtcgaggc gctcgttgtc gagcagttcg atcgcgcggc
    26341 gccgctcggc gtcggcgatt gcaaggtcgg cgggaactac gcggcgggcc ttgccggaag
    26401 cgaatacggg aagaagcacg gcttcccgat cgtccttttt ctcgatccgc gtgaaaaggt
    26461 gtatctcgac gagttcggga catcgaatgt cgtcgcgatc cacggcgacg cgtatgtgac
    26521 gcccaaatcc ccgtcgattc tgcccagcat caccaaccgc agcctcgcgg tgatcgcgga
    26581 ggacctgggc atgcgcgtcg agtggcgcca gatcgcgatc gacgaggtgc agtatttttc
    26641 ggagatcggc gccgtgggca ccgcggccgt gatcacgccg gtgtatcgga tccgctaccg
    26701 cgatcgcgac ttcaccttcg gaaaaccgga cgtcgccggc cccatgctca ccaaactcta
    26761 cgagcagtta acgggaatcc agtacggtga aatcgaggat cggcacggat ggcttgatgt
    26821 gatcgaggtg tgaaggggct gcgaactcgt gtggtccgtg gatgaaaccg aacggccgcc
    26881 gggaaacggc ggccgttcgt attattccga tttgacgacc cgttcggcct gaagggccag
    26941 gtactgcacc agcttttgtt cggaaatgaa tccaaggttc accaggatga tcccgatcag
    27001 tccgccctcg tttttctgca cctcgagcgc cttgttgagt tgatcatcgg taatctcgtt
    27061 attctccaat aagagctcac ctagaagcgg tcgtgccatt atattcctcc accatgtcga
    27121 tcatttcaaa atattcaacg ccgttacgta tttggaaaca aatcgcgaat aaatcaattg
    27181 attttttccg aaatggttaa aaatcaatct gccggttcgc ctcacatcaa cagccgctga
    27241 atggcctgct tccaaatgcg tcgctggcgt tcgcgctcgt ttggcgccat gtccggatcg
    27301 aaccgcgctg tgatcgcatt gtgtttggcc acctcgtcga tggagttgta gagccggctg
    27361 gtgaggccgg tgagatacgc agccccgagc gccgtcgact ccgcgacctc cggcagaagg
    27421 accgggatgc ccaggatatc ggcctggaac tgcatgagga aacggttggc ggtcgcgccg
    27481 ccgtcgacgc gcaattcgtg gatcgttttc ccggagtcgt tctgcagcgc gtcgatcacg
    27541 tccatcgatt gaaaggcgac cgccttgagg gaggcgcgga cgatctgctc gcgcgtggtg
    27601 tcgcgcgtaa gcccgaagat ggcgccgcgc gcgtccatct tccagtaagg ggcgcccagg
    27661 cccacgaagg ccgggatcac caccacctcg tcctccttgt ccaccgcgag ggccatgggc
    27721 tgggagtcgg cgctcgagtc gacaaacttc atgtagtcgc gcagccattg caccaccgcg
    27781 ccgccgatga acaccgcccc ctcgagcgca taggccggct ttccgtgctt gtcgcaggtg
    27841 agcgtggtga ggagcccgtg ccgggaaatg gtgtgctggg agccgatatt gagcagcatg
    27901 aaacacccgg tcccgtaagt gttcttgatg gtcccctcga acacgcagtt ctgtcccacc
    27961 atggcggcct gctggtcgcc ggcgatgccg ccgatggtta cgccgtcgg
//
LOCUS       DAORHF010000019        27333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1078_length_27333_cov_6.113278, whole genome shotgun sequence.
ACCESSION   DAORHF010000019 DAORHF010000000
VERSION     DAORHF010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27333
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1078_length_27333_cov_6.113278"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PKJ16_03040"
     CDS             <1..550
                     /locus_tag="PKJ16_03040"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HNV45990.1"
                     /translation="ADMSSYHRYELTDSGVSPQAAPGISKHVVVVDSDEHDVDGHLTE
                     KPIIAEQMCQKRLRKIASLKSSLDFEPIVEGQKSSPHLVISWGSTYETLREARNHLKK
                     KNALFAHLHLSQVWPLPVERLKALFARYDRIILVENNAVPELGSLLCECTQRAHDVFI
                     NRFDGRPFSVEEMVSRLTEVLP"
     gene            547..1404
                     /locus_tag="PKJ16_03045"
     CDS             547..1404
                     /locus_tag="PKJ16_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV45991.1"
                     /translation="MTVRIDDYRSAVENHWCPGCGNFGILEAVKKSLVQLDLPPEKVV
                     VCTGIGQAPKLPHYLRVNTFNGLHGREVASATAIKCAAPDLTVLVHAGDGGAYGEGGN
                     HFLHAIRRNIDITLVVHDNKVYGLTKGQASPMTTRGTKAKIHPHGVPSNALNPLALAI
                     AQNCGFVAQCMSARGDHLAATLSAAIRHKGFSLVHVLQPCVSWDKVHTYAYYKEHCYE
                     LGADYDPGDRMKALELVLAEGDRLPLGILYRTEQPTFEDLALSHLDAPLRDLPVDSGR
                     IERLMPKFQ"
     gene            complement(1510..2634)
                     /locus_tag="PKJ16_03050"
     CDS             complement(1510..2634)
                     /locus_tag="PKJ16_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV45992.1"
                     /translation="MTILIKAGIAIAAAGGLIVAVSQCARHVQQRTGDNGFPLPRTAS
                     PNFDGRRFRNAAATTISKPGTLLRSAAEFITGGQVRRPPVAPGPFRLDAAPAAGPAHD
                     GLRITWMGHSSALIEIGGKRFLTDPVWSDRASPVGFAGPQRFFAPPCAIEELPPLDGV
                     IISHDHYDHLDRETVTRLAARGVDFYVPLGVGPILAGWGVPQRQIRELDWWAEAPLGP
                     THRLVATPAQHFSGRLFINGNGTLWCSWVIIGPAHRVFFGGDGGMFDGFAEIGRRHGP
                     FDVTMLEIGAYHHNWRDIHLGPHLAIDAHLALRGKALLPIHWGTFTLAFHDWDAPIRE
                     LIDAAAPRGIALLAPAPGAAIIPDGTSRISRWWERDGSAL"
     gene            2848..3348
                     /locus_tag="PKJ16_03055"
     CDS             2848..3348
                     /locus_tag="PKJ16_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV45993.1"
                     /translation="MDIVNVDPQDCPYYLLTRASLAITAAFRKAFTGAGVEDVRPAYL
                     GALMCLWRECCVDEALGKFGGEGGMKLADLGRCAGLEPSTMTGLIDRMERDGLVRRED
                     DRSDRRVQRIVITEKGDALRAAVMEAVTATLRRSFDGIDMNELDVFKKVLRTVLSNAN
                     RGESNE"
     gene            3341..4387
                     /locus_tag="PKJ16_03060"
     CDS             3341..4387
                     /locus_tag="PKJ16_03060"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV45994.1"
                     /translation="MNEKRRIGYTCAYTPLALIHAAGFSPYRILPDVDCADRAGQVLH
                     DNLCPHVKRVLDRAMAGSVPELAGAVFMNSCDAMRRLADAWAAVRRDVPAALVDLPVV
                     VNDRTVSFFRDELERCAGILQSWGGVIMSDASIAEGVGTHNRIAERLERLRALAREGR
                     LPGGFPALQEAYNLASTAPPEEAIARLDHIESSALAAPSGDGIPVFVFGNVLPEPEAF
                     TLFEDAGARVVGEDLCTGSRLFARIDAAGPDPLRAIASSLLGRTACARTFDAEHPGGL
                     AETVVTRAREFGARAVIAYTAKFCDPYIARMPAVRDALRTEGIPFLALEGDCTLRSIG
                     QHRTRLEAFIEMMR"
     gene            4393..5619
                     /locus_tag="PKJ16_03065"
     CDS             4393..5619
                     /locus_tag="PKJ16_03065"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV45995.1"
                     /translation="MMHEFFSNLESGLSKAIDAGEASPRKIYARELARLGTRLYGGNG
                     GVAWCGITAPFDLLNAMGVTSCFVEFVGAMLASTGVVGSFIEETDHMGYSSDACSYHR
                     AVMGASRKGMVPEPAFLVGTTNPCSGGLAVIENLARRFGKRQYVLHIPQDQSGGNVRF
                     LAEQLRDLSAFVAAHTGAPLDPDALRRAVSNTNEARALMAEAYEFAKAVPSPVATKDL
                     ANYGIVMALFLGTDAAVDIARAFRDEFAKRVADTKSGVPNEKVRLLWIQNRIQFKNGI
                     DDILAREYGASVVIDELNDITWDAIDPDEPFEGIARRSISIPFNGSIANRVAHLKKLA
                     REYRVHGAVNPCNWGCRQGTGARGLIEEGLKEIGVPVLNLEVDCIDSRKFTEGQFRTR
                     IEAFMEMLAGRPSPWS"
     gene            5657..6460
                     /locus_tag="PKJ16_03070"
     CDS             5657..6460
                     /locus_tag="PKJ16_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV45996.1"
                     /translation="MYFLGIDIGSLSCDAVLIDDDENVIASSVVPTGARNRESIARVS
                     AEVLRDAGVDRGAVRATVSTGYGRDRVDDRLASVTEITCHARGIAKLVPGTRLLVDIG
                     GQDSKAILMARDGRVVDFAMNDKCAAGTGRFLEAMARALQVDIDEMGPLAAGAEGRQT
                     ISSMCTVFAESEVVSLIADGTPVNEIVHGINRSIAARVAGMVKRIAPDHGALPAAMSG
                     GVARNCGVVAALRGALGCEVAVPALPDTVGALGAALIARERCAAGAAAV"
     gene            complement(6471..7007)
                     /locus_tag="PKJ16_03075"
     CDS             complement(6471..7007)
                     /locus_tag="PKJ16_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV45997.1"
                     /translation="MWSGDRVQLAIDFAASAHDGQTVPGRGYAYIVHPLSVCAEVMRS
                     LAREGSDRPDLAVLCAILHDTLEDTAATYQDIVGSFGRDIADGVRALSKDPTLPKEAR
                     MADSLRRIREQPREVGIVKLADRIVNLQEPPEEWDRPKRAAYRDEAIVIRETLGNCSA
                     HLAARLDEKIRAYEAFLR"
     gene            7170..9287
                     /locus_tag="PKJ16_03080"
     CDS             7170..9287
                     /locus_tag="PKJ16_03080"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV45998.1"
                     /translation="MMHRAKRNRLANETSPYLLQHAENPVDWHPWGDEAFALARALDR
                     PVFLSIGYSTCHWCHVMERESFEDDAVAALMNRTFVCVKVDREERPDVDRLYMDFCQA
                     MTGAGGWPLTIVMTPEKKPFFAATYLPRTARYGRTGMMELVARIQALWREDRPRIEET
                     AEQVAAELARHAGAVSRSPGAGPSADAAFASLGRHFDRAHGGFGGAPKFPTAHVVSFL
                     ARYGARTGRAEAREMAEKTLRAIRRGGIYDQLGFGVHRYATDAEWRTPHFEKMLYDQA
                     ALARASLDAFRAFGGEAHASMAREIFAYVLRDLAAPDGAFYTAEDADSEGVEGRFYLW
                     RREEIVRIAGAARADWFARIYGAGPSGSFRDESDVGDDGGIVLRMTGEVDEIARASGA
                     DAAEVRAMLEEARQLLFEARERRVRPFRDEKVLTDLNGLMIGALAHGAIALDDPSLAL
                     IACRAADVILAERMTADGGLVHGNAGARPIPGMADDYAFLIEGLIALYGATFRAVYLE
                     RASVLNDFFIAHFWDDATGAFFSTADDEGGDLLFRKIELADGAIPSANSVAMGNLSRL
                     ARLTGRGELAEMAVRIAGTYPDGLSEHPSACAGMLAEKEFMMAAPCDIVIAGDPESDA
                     VRDLIRAARAGAPPQALLLVAGPAGGSADLAGIVENASGYIYPESGAAAYVCGNFSCR
                     EPVYTPEALSTLLQAITGDDNGS"
     gene            9277..9660
                     /locus_tag="PKJ16_03085"
     CDS             9277..9660
                     /locus_tag="PKJ16_03085"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV45999.1"
                     /translation="MDLKDYFETRQGTGVLATADAQGNVDAALYSRPHVMDDGTVAFI
                     MNDRVSHRNLQSNPRAAYLFTESENRRDGIRLYLVKSRETDDPAAIEPLRRHRSAGGD
                     DGKKLFLVVFTVERTRPLVDAAPSG"
     gene            complement(9652..10308)
                     /locus_tag="PKJ16_03090"
     CDS             complement(9652..10308)
                     /locus_tag="PKJ16_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV46000.1"
                     /translation="MLKRFRYFLGIHLLPALVYRIIRLYSATFRLTVHNEEAGMKHLK
                     EGGVILLCCLHQQFFAAIRYYKTYAKHHPGLMISKSKDGEVIAQVANRTGWVTVRGSS
                     SRGGAEALKDMIAHLKEHRLAAHILDGPRGPIGIVKPGLIRLAQEAKARIVPFYIQVD
                     RAWFFKSWDRFMLPKPFSRVKLIYDDMIQLEEITSDEDFERQRLHVENLMKPRLYPLS
                     "
     gene            complement(10520..10711)
                     /locus_tag="PKJ16_03095"
     CDS             complement(10520..10711)
                     /locus_tag="PKJ16_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46001.1"
                     /translation="MRVHLFDMDSLESRGMIVADDHHRWEYRDVANEHLVRTTSGLPL
                     RAVLACLSSFNIVYDIVED"
     gene            complement(10742..11356)
                     /locus_tag="PKJ16_03100"
     CDS             complement(10742..11356)
                     /locus_tag="PKJ16_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46002.1"
                     /translation="MKPIPFIIVLAATIASALPARADSPLTATEFSAAYQDHAVVREA
                     AKKEELDRALFDVLAAPGTPIDIKAAVINALGWKIEGRRNSQVFLSMLSERHKKKLDS
                     VSVKTLSADELFCLGYLMAMDDYLHPEKAIPILTLAHYKNRASFTIAIVLALARAQRA
                     MDRSWCEVWRVVEAVLDNGALTMDMREPARKIIVDYMALYKDEC"
     gene            11502..12548
                     /locus_tag="PKJ16_03105"
     CDS             11502..12548
                     /locus_tag="PKJ16_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46003.1"
                     /translation="MKYFAYAVVLIALLSGAMVLSQGRTDDVRTPDATRETKPDEPAR
                     APDTRKPDADAQKRPVDDPGATEQPAKKDADTAAPSDDAKKTVEPGKGPDTRTAEPEK
                     KDTTEHIIIDRKDRPEDHAPERPAEKKDRRDKTDDDAPSRTRRLGDEGKFIFGAYAGY
                     GFVYPSGLIAGTGDQGADVTAGYAVGGELGYMLLEGGGLFLGVEYASKPISINRTYMR
                     LSQQVQYQLAFIDYVLTYRGFFDAFDTRKSRLFAEIGMFYGSRSGRWVMRETMGSTTY
                     ERDMATLKPGKVQNEYGICFGFGVQFDAARDVSVNVGLRFEISLQNAFEYEDELKTNL
                     GMLTLGACYKFDPF"
     gene            complement(13129..13308)
                     /locus_tag="PKJ16_03110"
     CDS             complement(13129..13308)
                     /locus_tag="PKJ16_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007933562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46004.1"
                     /translation="MQTIKQEALAAISKLPDNATIEDMMYRLYVIDKIHAGLDDIRNG
                     RVITAEELKREIQTW"
     gene            complement(13497..14201)
                     /locus_tag="PKJ16_03115"
     CDS             complement(13497..14201)
                     /locus_tag="PKJ16_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV46005.1"
                     /translation="MAILPTEARPAERGDRHAQAPALSPVKPAAIYHGSRRTPLVALT
                     FDACERINEKTGYDRRIIDILTRTRTPATFFLGGRWMLSHPEETRELARVPFFELANH
                     SYSHPDMRALGDAALRYEIQETQNIMHRIAGRQGTLFRAPFGWYNKRLVAAAGALGLR
                     LIQWDVVSGDPDPRVSAAMIRRFVLGGARFGSIAIMHVNGRGHHTHAALPGIIDELRR
                     RGFRFVTVSELLSVAP"
     gene            complement(14291..15058)
                     /locus_tag="PKJ16_03120"
     CDS             complement(14291..15058)
                     /locus_tag="PKJ16_03120"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HNV46006.1"
                     /translation="MFPILFQYKFLQIGGYGVMLGMGFYLAFLLLERELKLRDINPEL
                     AYRILLGAIPGGIVGSKMFHIFEHWSDFTSSWQSAKSMIFSGEGLSAYGGFVLAAIIC
                     FIIIRKSGEDFLTVGDAVAPSMALGYCFGRFGCHVAGDGCYGVVSTLGIATPYPNGIV
                     PTTTPVLPTPFFEVYFSFIVFALLMQLRKLDWPVGRIFFLYMILNGLPRFLVEFVRLN
                     PRGPLGLSQAQVIGILLIIGGIAGWIWTGRRKAAAEA"
     gene            complement(15086..15433)
                     /locus_tag="PKJ16_03125"
     CDS             complement(15086..15433)
                     /locus_tag="PKJ16_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HNV46007.1"
                     /translation="MGCLFCNIIAGEIPSTKIFENERVFAFADISPQAPVHALVVHKT
                     HTTNLDELTAENSGIMADLFLGVKEVARITGVDAKGYRVIINNGAAGGQVIWHLHVHV
                     LGGRDDMGPMVVK"
     gene            15596..16708
                     /locus_tag="PKJ16_03130"
     CDS             15596..16708
                     /locus_tag="PKJ16_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46008.1"
                     /translation="MKTIDDIRAIVYDPAEERAPLVARFLALPGKRHLACTDRESVTR
                     ALDGLRAEGLDSKEALVLQRFMGRFFQKCPGSPRVICCNYHLINTCFNCLYDCAYCYL
                     NTYLNSFGVVQFTNILDVADEIEAHVAARPAGAVLRIGTGEFTDSLMMDEVTGIAASL
                     IARMSRFPNVFLELKTKSDNVGHLLALPEKGNAVLAWTLNTERNIALYENGTAALDRR
                     IGAARAACAAGYRIAFHFDPMIAGEGWEAEYRAVAARALAAVDPARVAWVSLGGFRYA
                     SGFREIIRARFPDEALTRAEMFPGIDGKYRYLRPRRLAMYRAMRECINELAPDAFVYL
                     CMEGADMWEDFAGARYASSDDLEEDIARHLRRTVLR"
     gene            complement(16705..17412)
                     /locus_tag="PKJ16_03135"
     CDS             complement(16705..17412)
                     /locus_tag="PKJ16_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HNV46009.1"
                     /translation="MRALAVVIAALATCAGCVSSITRYTIPRTPPPEGFYVTVNKGSI
                     AATDHVLSATSASIKRGTDTGAVTGDDAVPRRESAPADKKDEGPSKKSDGVPPAPDGD
                     TKGANDAARDAAAPSDKAARDAPADGKKPADTTDDTAKSAVKESEKETKPVDAKDAAA
                     DEKARQATTDSVRTGRSSSQGVLGLVAWGDSSIRKAADEAKITRVHSVDYERVMLRLP
                     YFNWLIYESLTVIVTGD"
     gene            complement(17414..17731)
                     /locus_tag="PKJ16_03140"
     CDS             complement(17414..17731)
                     /locus_tag="PKJ16_03140"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HNV46010.1"
                     /translation="MRMKPIAVMCAAVLVIGVLGCSSTPTPKGCIYTKVQGPFTVTAN
                     LNSPKVGRATASSVLGIVAWGDSSIKKACESAGITKIHHVDYEDFNILLVYMTYTTVV
                     YGE"
     gene            complement(17848..18237)
                     /locus_tag="PKJ16_03145"
     CDS             complement(17848..18237)
                     /locus_tag="PKJ16_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004478751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV46011.1"
                     /translation="MGKRIASFSKGKRIIKSGKYEQRMYVILEGEVEISFTVNDEKVS
                     VARLGKGDFFGEVSLFQEIERTANAYALTDVRATFIDSPEELVAFITKNPRYAVKMVR
                     TLTERIANTDRLLVGELCGNRVVKYVW"
     gene            18452..18895
                     /locus_tag="PKJ16_03150"
     CDS             18452..18895
                     /locus_tag="PKJ16_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNV46012.1"
                     /translation="MDKSETAVQTFGAGFNCAQAVLGAFASEAGLDADTAYAIATGFG
                     AGLAYRGETCGAVSGAVMVLGLRYGWRGGDIAESRDRTVERVREFIRRFEGTHGTILC
                     QDLLGMDVADPAALCKARERNLFRTLCPRYVRDAAAILEDMLGAP"
     gene            18892..19608
                     /locus_tag="PKJ16_03155"
     CDS             18892..19608
                     /locus_tag="PKJ16_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HNV46013.1"
                     /translation="MIAHADITALLPGGEGDWIVGFADLGGLLHERFAGHPFAIVIGR
                     RLDDAIMDDVADGPVRAYYDHYRDINRRLSDAVRAVADRVRRDGFDCRSVEPTVLDEQ
                     VAADYMETMRLDFSHKMAATRAGLGWIGKTDLFVSERFGPRLRLATVLADREVAPAGT
                     PVTVSRCGACALCVERCPAKAATGKLWRITVDRDEFYDPWKCREKCRELARTRIGVNE
                     SICGICVSVCPVGRAGAGAI"
     gene            complement(19601..20278)
                     /locus_tag="PKJ16_03160"
     CDS             complement(19601..20278)
                     /locus_tag="PKJ16_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46014.1"
                     /translation="MNPTARNKRIATGVLLVIGGMMMALPATLLWSALAGPGALVKER
                     SGDFYRARAVLVAGPLIVCRRDAASHKTKRAALDSRMERTNLDAIGLHRIEYIEFIQP
                     PVETAAGPAHQAPPDEYTGTYSINAAGNSGILALRRANGAFYGSIRFPNWGRGATEPL
                     KGVRISGGKIFFVRSVTTPDEVKRTGANGPFVQEYSGEYTQGGRSIRGSYRVSGIQKQ
                     WDASKVR"
     gene            complement(20321..22438)
                     /locus_tag="PKJ16_03165"
     CDS             complement(20321..22438)
                     /locus_tag="PKJ16_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV46015.1"
                     /translation="MKTTQPIVRLGTILLAGALATAAHAAPAVVLTEAAREYALGPSV
                     EYLEDRDGTIAFDDIRAGTHDERFVRNTAPVMNFGFTKSAYWMRVTVKNNHPAIQNWL
                     LEVGYPHHDYIRFYSPSPSGKYEMRTAGDRVAFNEREVRYHTFLFRLAIPRGSSMTVY
                     LRFQTESSFQVPLTLWEPTAFAETVNRSQYGFGVYYGIMIVMLLYNLFLFIGIRERVY
                     LIYILFIGAFILGQMSLNGFAFEYLWFAVPWWANVSVLFSLFLSIFFSILFCRDYLNT
                     KANVPAIDKVLLVLLGCSVLGMGMSIFAPYALGVKFVALMATVLAIPSIAAGIICMRK
                     GVPAAKYYLIAWLALLIGVMLLGLRNLGAVPTFFLTTYSIQIGSAMQVVLLSLGLADR
                     INTMRRERYLAQQEALESHREMLAAQEKLVEQLKETDRIKDEMNRDLERKVEERTAEL
                     KSARDQLWGEMQLAKKIQTVLLPDRPEIEGFQIAAYMQPADEVGGDYYDIINVDGLDW
                     LVIGDVSGHGVPAGLIMMMVQTAIHTALAGKPNLKPQRLLSHINQVVTANIKKLNEDK
                     YMTITVLACLKNGTFYFSGLHQDIMIYRAATKTVELVETDGIWIGLVEDINGMLHDSE
                     LTLSRGDVMFLYTDGITESWRRGARKDERDPESDMFGDERLKSCLLTLGEKEPEEIKR
                     GILDEMRDYMPADDVTLLIVKKG"
     gene            complement(22515..25088)
                     /locus_tag="PKJ16_03170"
     CDS             complement(22515..25088)
                     /locus_tag="PKJ16_03170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46016.1"
                     /translation="MGFFSTFYLNYFSVGMLIPVLFHLFLAIFLFSIRNRSKATFHLA
                     MGYALWVVFNLAYFAANSFYHPLAAYHRWFTVGSVLFIITHFNMIHFYFPDERAPRFG
                     RIYFIVSYAVAALVSLAFIASTIHGRTVYQINAHYWDFDADTFSKYFSLLMLLYIFIQ
                     LPLALWRTVTTKSRDRWISLTMGIFLFVSGLAPGVTNTLSRDGAMDRELYQVSWVIFN
                     VLCFAAAAIIYLNSTRDRTSFIGKLITITGVAFLLVVQFIAYFALTDKDRGYDFAHQH
                     LTARVLISDYRPDDFSYRATYALDTNRFIGIERAPGGGAAVDHASYRQEFINAALLGK
                     IRSLPTDGFQSRIMKLLDETPAAFAGYRAFIQNELGRMPREETRPARRLLARLDGAKR
                     IIAYRANKIRALPEKGFRESLVKFASKKEGDFEPFRAALMALMNDASRTDAELRRDAL
                     SLLEPMNGEGVRLYRTDPTGQHHYIAYVQFDPAANAVHEVGYSYRAYREFIHPFALTL
                     IIILAALLVIVRFGFQYFFMGALVAPLRTLSRGVRLVNNGDLDVTVPVRFEDEIGFIT
                     HAFNNMVGSIRDSKKKLEEYAQTLETKVEERTAELKSARDQLWGEMQLAKKIQTVLLP
                     DRPEIEGFQIAAYMQPADEVGGDYYDIINVDGLDWLVIGDVSGHGVPAGLIMMMVQTA
                     IHTALAGKPNLKPQRLLSHINQVVTANIKKLNEDKYMTITVLACLKNGTFYFSGLHQD
                     IMIYRAATKTVELVETDGIWIGLVEDINGMLHDSELTLSRGDVMFLYTDGITESWRRG
                     ARKDERDPESDMFGDERLKSCLLTLGEKEPEEIKQGILDAMRDYMPADDVTLLVVKRS
                     A"
     gene            complement(25241..26674)
                     /gene="gltX"
                     /locus_tag="PKJ16_03175"
     CDS             complement(25241..26674)
                     /gene="gltX"
                     /locus_tag="PKJ16_03175"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HNV46017.1"
                     /translation="MRLRFAPSPTGYLHIGNARTAILNYLIKRKTGATLVLRVEDTDM
                     ERSSRESEASILADLRWLGIEWDEGPDTGGAHGPYRQSERFDIYREYTEKLIASGSAY
                     PCYCTQDELDAMRREGREGAFEYPGTCRDLTAAERAEREAKGLKPTVRFRVPAGESVT
                     VNDLIKGPVAFSSENIGGDFIIVRSDGVPVYNYIVIIDDALMAITHVIRGEDHLSNTP
                     KQILIARALGLPVPEYAHLALVMGDDRKKLSKRHGITSVDLYRSDGYLPEALVNYIAM
                     LGWAAESGEEIMPLDDIVRHIEIGGLAKSAAVFDFQKLRWMNAHYIRSAGIGAITELF
                     VPHIEKAGYAVAGLDRAWLESVIALTRVYCEVLSDIGRIIGVFLEEVPALDDDAAAFL
                     DDDEARRVIAAAQDAIAASGAGADFAPALIAAIKGSGVKGKKLFLSMRAIFTGRLAGP
                     ELDQALPLIGFEKCAKRVEAARARCSA"
     gene            27044..>27333
                     /locus_tag="PKJ16_03180"
     CDS             27044..>27333
                     /locus_tag="PKJ16_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HNV46018.1"
                     /translation="MDSASEKTTSNFIKDIINEDRKNGKHGARVHTRFPPEPNGYLHI
                     GHAKSICLNFGLAKEYGGLCNLRMDDTNPSKEDVEYVDSIMADVRWLGFDWD"
BASE COUNT         4758 a         8286 c         9214 g         5075 t
ORIGIN      
        1 tgcggacatg agttcgtacc accggtatga actcacggac tccggggtct cgccacaggc
       61 ggccccggga atatcgaagc acgtcgtcgt ggtggactcc gacgagcacg atgtggacgg
      121 ccacctgacc gagaagccca tcattgcgga acagatgtgc cagaagcggt tgcgcaagat
      181 cgcctcgcta aaatcgtcgc tcgacttcga gcccatcgtc gagggacaga agtcgagccc
      241 gcacctggtg ataagctggg ggagcaccta tgaaaccctg cgggaggcgc ggaaccatct
      301 gaaaaaaaag aacgccctgt tcgcgcacct gcacctgtcg caggtctggc ccctgcccgt
      361 ggaacggctg aaggccctgt tcgcgcgata cgacagaatc atactcgttg aaaacaatgc
      421 cgtaccggag ctggggtccc tgctgtgcga atgcacgcaa cgggcgcacg acgtgtttat
      481 caatcgcttc gacgggcgcc ccttctcggt ggaagaaatg gtctcgcgtt taacggaggt
      541 gctgccatga ccgtgcgcat agatgattac cggagcgccg tggagaacca ctggtgcccg
      601 gggtgcggaa acttcggcat tctcgaagcg gtgaaaaaat ccctggtgca gctggacctg
      661 cctccggaaa aagtcgtggt gtgcacgggc atcggccaag cgcccaagct gccgcattac
      721 ctgcgggtga acaccttcaa cgggctccac ggccgggagg tcgcgagcgc cacggccatc
      781 aagtgcgcgg cgccggacct gacggtcctg gtgcatgccg gcgacggcgg ggcctacggt
      841 gagggcggca atcacttcct gcacgccatc cgccgcaaca tcgacatcac cctggtggtc
      901 cacgacaaca aggtctacgg gctgaccaag ggccaggcgt cgcccatgac gacgcgggga
      961 acaaaggcca agatccatcc ccacggcgtt cccagcaatg cgctcaatcc actggccctg
     1021 gccatcgccc aaaattgcgg tttcgtcgcg cagtgcatgt cggcccgggg ggatcacctg
     1081 gcggccacgc tgtcggccgc catccggcac aaggggtttt ccctggtcca tgtcctgcag
     1141 ccctgcgtga gctgggacaa ggtccacacg tacgcgtatt acaaggagca ctgctacgag
     1201 cttggcgccg actacgatcc gggcgatcga atgaaggccc tcgagctggt tctcgctgaa
     1261 ggcgaccggc tgccgctggg gatcctgtac cggacggaac agccgacctt cgaggacctg
     1321 gccctgtcgc acctggacgc gcccctgcgc gatcttccgg tcgactcggg ccggattgaa
     1381 cgcctgatgc cgaaatttca atgattggtt cgcccgcggt gcgggcccgc acgggcggtc
     1441 gttttcggtc acgcgttgcg tgcaacagga gtcgcgcgtg cgtttcgcat gcggcgcagt
     1501 gggctgccgc tacagcgccg atccgtcccg ctcccaccat cgcgagatgc gcgatgtccc
     1561 gtcggggata atcgcggcgc cgggcgcggg agcgaggagc gcgatgccgc gcggggcggc
     1621 cgcgtcgatg agctcccgga tgggcgcgtc ccaatcgtga aaggcgagcg tgaaggtgcc
     1681 ccagtggatg ggcagcaggg cctttcccct gagcgcgaga tgcgcgtcga tggcgagatg
     1741 ggggccgagg tggatgtcgc gccagttgtg gtggtaggcg ccgatctcga gcatagtcac
     1801 gtcgaagggg ccatgtcgcc ggccgatctc cgcgaagccg tcgaacatgc cgccgtcgcc
     1861 gccgaagaag acgcggtgcg cgggcccgat gatgacccat gagcaccaga gcgtcccgtt
     1921 gccgttgatg aacaaccgac cggaaaaatg ctgcgccggc gtcgcgacga ggcgatgcgt
     1981 cggccccagc ggcgcctccg cccaccagtc cagttcgcgt atctgccgct gcggaacgcc
     2041 ccagcccgcg aggatcggcc ccacccccag cggaacgtag aaatcgaccc cccgcgcggc
     2101 cagccgcgtc acggtctccc ggtccaggtg gtcgtaatgg tcatgggata tgatcacgcc
     2161 gtcgagcggc ggcagctcct cgatcgcgca gggcggcgcg aagaagcgct gcggtcccgc
     2221 aaaccccacg ggcgaagcgc gatcggacca gaccgggtcc gtgaggaagc gtttgccgcc
     2281 gatctcgatg agggcgctcg agtgccccat ccacgttatg cgcaatccat cgtgggcggg
     2341 tcccgccgca ggggcggcgt ccaggcggaa ggggccgggg gccacgggcg gtcggcggac
     2401 ctggccgccg gtaatgaatt ccgcggcgct cctgagcagc gtccccggct tgctgatggt
     2461 cgtggcggcc gcgttgcgga agcggcggcc gtcgaaattg ggcgatgccg tgcgagggag
     2521 cgggaacccg ttgtcgccgg tgcgctgctg gacatgccgc gcgcactgtg aaacggcgac
     2581 aatcaggcct ccggcggccg cgatcgcgat tcccgccttg atcagtatcg tcattgggca
     2641 ttgcctcccc gaggttggcc gcgcgaactt ccggtgcgca acaggcgcgg atctcgattt
     2701 ctatgcaatt tggtttcagt actggccgtt agtcattttt ttttcggtga tgttgcagaa
     2761 aaatcaatat tttggttgac gataatacgt atacgaaata tatttatatc aatcatactt
     2821 aaacgaatat tacacaaggg ttataaaatg gatatcgtga atgttgatcc ccaagactgc
     2881 ccgtattacc tgctcacccg ggcgtcgctt gccatcaccg cagcgtttcg gaaggccttc
     2941 acgggggcgg gcgtcgagga tgtgcggccg gcgtatcttg gcgcgctgat gtgcctgtgg
     3001 cgtgagtgtt gcgtggacga ggcgctgggc aagttcggcg gcgagggtgg catgaagctg
     3061 gccgacctgg ggcggtgcgc gggtctcgag ccgtccacca tgaccggcct catcgatcgc
     3121 atggagcgcg acgggctggt gcggcgcgag gacgatcgca gcgaccgtcg cgtgcagcgc
     3181 atcgtcatca ccgagaaggg ggacgcgctg cgcgccgccg tcatggaggc cgtcacggcg
     3241 accctccggc ggtccttcga cgggatcgac atgaacgagc tggatgtctt taaaaaggtc
     3301 ctccggaccg tgctgtcaaa cgccaatagg ggtgaatcga atgaatgaaa aacgacgtat
     3361 cggctacacc tgcgcctaca cgccgcttgc gctgatccac gcggcggggt tttcgccgta
     3421 ccggatcctg cccgatgtcg attgcgccga ccgcgccggg caggtgctgc acgacaatct
     3481 ctgtccgcac gtgaagcgcg ttctggaccg cgcgatggcg ggaagcgtgc cggagctcgc
     3541 gggcgcggtg ttcatgaaca gctgcgacgc catgcggcgg cttgcggacg cctgggccgc
     3601 cgtgcggcgg gacgttcccg cggcgctcgt cgacctcccc gtggtggtga acgaccgcac
     3661 ggtgtccttt tttcgcgacg agctggaacg gtgtgcgggg atcctccaat cgtggggcgg
     3721 cgtgataatg agcgacgcct cgatcgcgga gggcgtcggg acccataacc ggatcgccga
     3781 gcgcctcgag cgcctgcgcg cgctcgcgcg ggagggacgg cttcccggcg gctttccggc
     3841 gctgcaggag gcgtacaatc tcgcatcgac ggcgccgccc gaggaggcga tcgcgcgcct
     3901 cgatcacata gaatcaagcg cgctcgcggc tccctccggg gacggcattc ccgtgttcgt
     3961 gttcggcaat gtgctgccgg agccggaggc cttcacgctg ttcgaagacg ccggcgcgcg
     4021 cgtggtgggc gaagacctgt gcaccggctc gcgcctcttc gcgcgcatcg acgcggccgg
     4081 gcccgatccg ctccgcgcga tcgcgtcgtc gcttctcgga cggacggcgt gcgcgcgcac
     4141 cttcgacgcc gagcaccccg gcggtctcgc ggagacggtc gtgacgcgcg cgcgggagtt
     4201 cggggcccgc gcggtgatcg cgtacacggc caagttctgc gacccgtata tcgcgcgcat
     4261 gcctgcggtc cgcgacgcgc tccggaccga ggggatcccc tttctggccc tcgagggcga
     4321 ctgtacgctg cgctccatcg gccagcaccg cacgcgcctc gaggcgttca ttgagatgat
     4381 gaggtaatcg ccatgatgca tgaattcttt tcgaaccttg aatcggggct ctcgaaggcc
     4441 atcgacgccg gagaggccag cccgcggaaa atctacgccc gcgagctcgc gcgcctgggc
     4501 acacggctct acggcggcaa cggcggcgtc gcgtggtgcg gcatcaccgc gcccttcgat
     4561 ctgctcaatg cgatgggcgt cacctcgtgc ttcgtcgaat tcgtcggcgc gatgctcgcc
     4621 tcgacgggcg tggtggggtc cttcatcgag gagaccgacc acatgggcta ctcgtccgac
     4681 gcgtgcagct atcatcgcgc ggtcatgggc gcgtcgagaa aggggatggt gccggagccc
     4741 gctttcttgg tggggaccac caatccctgc tcgggcgggc tcgccgtcat cgagaacctc
     4801 gcgcgccggt tcgggaagcg gcagtatgtg ctgcatatcc cgcaggacca atcgggcggg
     4861 aacgttcgtt ttctcgccga acagcttcgc gacctgtcgg cgttcgtggc cgcgcacacc
     4921 ggcgcaccgc tcgatcccga cgcgctccgg cgcgccgtgt cgaacaccaa tgaggcgcgc
     4981 gccctgatgg cggaggccta cgagttcgca aaggcggtgc cctcgccggt cgcgacgaaa
     5041 gacctggcca actacggcat tgtgatggcg ctgttcctgg gaacggacgc ggcggtcgat
     5101 atcgcccgcg catttcgcga cgagttcgcg aagcgcgttg cggacacgaa aagcggcgtg
     5161 cccaacgaga aggtgcgcct gctctggata cagaatcgca tccagttcaa gaacgggatc
     5221 gacgacatcc tcgcccgcga atacggcgcg tccgtcgtca tcgacgagct caacgatatc
     5281 acgtgggacg ccatcgatcc ggacgagccg ttcgagggca tcgcgcggcg ctccatctcg
     5341 atcccgttca acggttcgat cgcgaaccgc gtggcgcacc tgaaaaagct cgcgcgcgag
     5401 tatcgggtcc acggggccgt caatccctgc aactggggct gccggcaggg gaccggcgcg
     5461 cgcggtctca tcgaggaggg cctgaaggaa atcggggtcc cggtgctcaa cctcgaggtg
     5521 gactgcatcg attcgcgcaa gttcacggag gggcagttcc ggacccgcat cgaggcgttc
     5581 atggagatgc tggccggtcg cccgtcgccg tggtcgtaag atacgatcta tcattttatc
     5641 atcgtggggt aatgccatgt attttctggg aatcgatatc ggaagtttgt cctgtgacgc
     5701 cgtgctgatc gacgacgacg agaacgtgat cgcctcgtcg gtggtcccga ccggcgcgcg
     5761 caaccgcgag tccatcgcgc gcgtttcggc cgaggtgctg cgcgatgcgg gagtcgatcg
     5821 cggcgccgtc cgcgccacgg tctcgaccgg ctatgggcgc gaccgggtgg acgaccgcct
     5881 ggcgtccgtg accgagatca cctgccacgc gcgcgggatc gcgaagctcg tcccggggac
     5941 gcggcttttg gtggacatcg gcgggcagga ttcgaaggcg atcctcatgg cgcgcgacgg
     6001 ccgggtggtc gatttcgcga tgaacgacaa gtgcgcggcc ggcaccgggc gcttcctcga
     6061 ggcgatggcg cgcgccctgc aggtcgacat cgacgagatg gggccgctcg cggcgggcgc
     6121 cgagggacgg cagaccatca gcagcatgtg cacggtcttc gccgagagcg aggtggtctc
     6181 gctcatcgcc gatgggacgc cggtgaacga gatcgtccac gggatcaacc gctcgatcgc
     6241 ggcgcgcgtc gcggggatgg tgaagcggat cgcgccggac cacggcgcgc tcccggccgc
     6301 gatgtccggc ggcgttgcgc gaaattgcgg tgtggtggcg gccctccgcg gcgcgctggg
     6361 gtgcgaggtc gccgtgcccg cgctccccga taccgtgggg gcgctcggcg cggccctcat
     6421 cgcgcgcgag cgctgtgcgg cgggggcagc ggccgtctag ggggcgcgcg ttagcgcagg
     6481 aatgcctcgt acgctcgtat cttctcgtcg aggcgcgcgg cgagatgggc gctgcagttc
     6541 ccgagtgttt cgcgaatgac gatcgcctcg tcgcgatagg ccgcgcgctt cgggcggtcc
     6601 cactcctccg gcggctcctg gaggttgacg atgcggtcgg cgagcttgac aattcccacc
     6661 tcgcgcggct gctcgcggat gcggcgaagc gagtcggcca tgcgcgcctc cttcgggagc
     6721 gtcgggtcct tggaaagcgc ccggacgccg tcggcgatgt cacgcccgaa cgacccgacg
     6781 atatcctggt aggtcgcggc ggtgtcctcg agcgtgtcgt ggaggatggc gcagaggacg
     6841 gcgaggtcgg gacggtcgga tccctcgcgc gcgagggaac gcatcacctc ggcgcagacg
     6901 gacagcggat ggacgatgta ggcgtatccc cgtccgggga cggtttgccc gtcatgcgcg
     6961 ctcgccgcga aatcgatcgc cagttgcacc ctgtcccccg accacatggc attcctcctt
     7021 cggttgggaa tcaacgagca cgcatgcagt gaaatgtcaa cagcttggcg gtgattccgt
     7081 tcggtttctc gccgtattcg cgacgatcat ttttggcgta tcggatacga cgtgctacgc
     7141 tggtcttcgc cgaatgaacg agggcgatga tgatgcaccg cgcgaaacgc aaccggctgg
     7201 cgaacgagac cagcccctat ctgctccagc acgccgaaaa cccggtggac tggcacccgt
     7261 ggggcgacga ggccttcgcg ctcgcccgcg cgctggaccg cccggtcttc ctctcgatcg
     7321 gctactcgac atgccactgg tgccacgtca tggagcgcga atcgttcgag gacgatgcgg
     7381 tggcggcgct catgaaccgc acctttgtgt gcgtcaaggt ggaccgcgag gagcgtcccg
     7441 acgtcgaccg actgtacatg gacttctgcc aggccatgac gggcgcgggc ggctggccgc
     7501 tcaccatcgt catgacgccc gagaagaaac ccttcttcgc ggcaacctat ctgccccgca
     7561 ccgcgcgtta cggcaggacc ggcatgatgg agcttgttgc gcgcatccag gcgctctggc
     7621 gcgaggacag gccgcggatt gaagaaaccg cggaacaggt agccgccgaa ctcgcgcgtc
     7681 atgcgggggc ggtctcacga tctcccggtg cgggcccctc cgccgacgca gcgtttgcct
     7741 ccctggggcg gcatttcgac cgcgcccacg gcggattcgg cggcgcgccg aaattcccca
     7801 cggcccacgt ggtgtcgttc ctcgcgcgct acggggcccg gaccggccgc gccgaggcgc
     7861 gcgagatggc ggagaaaacg ttgcgcgcga tacggcgcgg cgggatctac gaccagctgg
     7921 gcttcggcgt ccaccggtat gcgaccgacg ccgaatggcg caccccgcat ttcgagaaga
     7981 tgctctacga ccaggcggcg ctcgcgcggg ccagcctgga cgccttccgg gcattcgggg
     8041 gcgaggcgca tgcgtcgatg gcgcgcgaga tattcgccta tgttctgcgc gacctcgccg
     8101 cgcccgacgg cgccttctac acggccgagg acgccgacag cgaaggggtc gagggccggt
     8161 tctatctctg gcgtcgcgag gagatcgtgc gcatcgcggg cgcggcgcgc gcggactggt
     8221 tcgcccgaat ctacggcgct ggtccgagcg gaagtttccg agacgaatcg gacgtcgggg
     8281 acgatggcgg tatcgtcctg cgcatgacgg gtgaggtcga cgagatcgcg cgcgcgtccg
     8341 gcgctgatgc cgccgaggtg cgcgcaatgc tcgaggaggc gcggcagttg ctctttgagg
     8401 cgcgcgagcg acgggtgcgg ccctttcgcg acgagaaggt gctcacggat ctgaatggcc
     8461 tcatgattgg cgcgctggcg catggcgcga tcgcgcttga cgatccctcg ctcgcgctca
     8521 tcgcgtgccg ggcggccgat gtcatcctcg ccgagcggat gaccgctgat ggcgggctcg
     8581 tgcacgggaa cgccggtgcg cgcccgatac cgggcatggc ggacgattac gcctttctca
     8641 tcgagggact gatcgcgctc tatggggcga cctttcgggc ggtgtacctc gagcgcgcca
     8701 gtgtgctgaa cgattttttc atcgcgcatt tctgggacga cgcgaccggc gccttcttct
     8761 cgaccgcgga cgatgagggc ggcgatctgc tcttccggaa aatagagtta gccgacggcg
     8821 ccataccctc ggcgaactcg gtcgcgatgg ggaacctgtc gcgcctggcc cgactgaccg
     8881 ggcgcggcga actggctgag atggccgtgc gtatcgcggg cacctatccc gacgggctct
     8941 cggaacatcc gagcgcctgc gccgggatgc tcgccgagaa agagttcatg atggcggcgc
     9001 cctgcgacat cgtcatcgcg ggcgatccgg agtcggacgc cgttcgcgat ctcatacgcg
     9061 cggcccgagc gggcgcgccg ccgcaggccc tcctgctcgt tgcgggcccg gcgggcgggt
     9121 cggcggatct tgcgggcatc gtcgagaacg cgtccgggta tatatatccg gagtcaggcg
     9181 cggcggcgta tgtgtgcgga aatttttcct gtcgggaacc ggtatatacg ccggaggcgc
     9241 tatcaacact attacaggca ataacggggg acgataatgg atcttaagga ttatttcgag
     9301 acgcgccagg ggaccggcgt gctggccacg gcggacgcgc aggggaacgt cgatgcggcg
     9361 ctgtattcac gcccgcatgt catggacgac ggcacggtcg ccttcatcat gaacgatcgg
     9421 gtgagccatc ggaacctgca gtcgaatccc cgggcggcct atctgttcac ggaatcggaa
     9481 aaccggcgcg acggcatacg gctgtacctg gtgaagtcgc gggagacgga tgatcccgcc
     9541 gcgatcgagc cgctgcgtcg gcaccgatcc gccggcggcg atgacggcaa gaagctcttc
     9601 ctagtcgttt ttacggtcga acggacgagg ccgctcgtgg atgcggcgcc gtcaggataa
     9661 gggatagagc cgcggcttca tcaggttctc gacgtggagc cgctggcgct cgaaatcctc
     9721 gtcgctcgtg atctcctcga gttgtatcat gtcgtcgtag atgagcttca cgcgcgagaa
     9781 gggcttgggc agcatgaagc ggtcccagct cttgaagaac catgctcggt cgacctggat
     9841 gtaaaagggt acgatacgcg ccttcgcctc ctgcgccagc cggatgagcc cgggttttac
     9901 gatgccgatg ggcccgcgcg gcccgtcgag gatgtgcgcc gccaggcggt gttccttcag
     9961 gtgggcgatc atgtccttga gcgcctcggc gccgcccctc gatgacgatc cgcgcacggt
    10021 gacccagccc gtgcgattgg cgacctgcgc gatcacctcg ccgtccttgc tcttgctgat
    10081 catgagcccc ggatgatgtt tcgcataggt cttgtagtag cggatggccg cgaagaactg
    10141 ctggtgcagg cagcacagca gaatgacgcc cccctccttg aggtgcttca tccccgcctc
    10201 ctcgttgtgc accgtgagcc ggaaggtcgc ggagtacagg cgaatgatcc gatagacgag
    10261 cgcggggagc agatgaattc ccagaaagta gcgaaagcgt ttcagcatga ttagactcct
    10321 tgccggacag gcgtgcgagc gcatatgcgt atggacattc gccggcgaga gaatgaacat
    10381 gccatgcacg agtggctcac tgtgagatcg cgggagcggc gcatgggccg ctgtcccgct
    10441 tccgtggctc tgagaccatg tcgccgtgga aaatcaaaaa gaacacgcgg cgaacggcgt
    10501 gcgatacatc gaccgacgat cagtcctcga cgatatcgta gacgatattg aacgacgaaa
    10561 ggcacgcgag cacggcgcgt aacggcagac cggacgtggt gcggacaaga tgctcgttgg
    10621 cgacgtcgcg gtactcccag cggtgatggt cgtcggcgac gatcatcccg cgcgattcga
    10681 gagaatccat gtcgaagagg tgtacgcgca tgtccggttc cgtgtcgcgg cggcgccgcg
    10741 atcagcactc atccttgtag agggccatgt agtcaacgat aattttccgc gccggttcgc
    10801 gcatgtccat cgtgagcgcg ccgttgtcga ggaccgcctc caccacgcgc cacacctcgc
    10861 accagcttcg gtccatggcg cgctgcgcgc gggcgagcgc gaggacgatg gcgatggtga
    10921 acgaggcgcg gttcttgtag tgcgccagcg tgaggatggg gatcgccttc tcggggtgca
    10981 ggtaatcgtc catggccatg aggtagccca ggcagaagag ctcgtcggct gagagcgttt
    11041 tcacggaaac cgaatcgagc ttttttttgt gccgttcgga gagcatcgag aggaaaacct
    11101 gcgaattccg ccgcccctcg attttccaac ccagcgcgtt gatgacggcc gccttgatgt
    11161 cgatgggggt gccgggggct gcgagaacgt cgaagagcgc gcggtcgagc tcctcttttt
    11221 tcgcggcctc gcggacgacg gcatggtcct ggtaggccgc ggagaattcc gtcgcggtga
    11281 gcggggaatc ggcccgggcc ggcagcgccg acgcgatcgt cgcggcgaga acgatgatga
    11341 acgggatcgg tttcatggat gcgcccatct cctatcgtca ctaagatatg cgctctgcgc
    11401 cggtgcggca agaaaaatat ccccgaagcg catttgttat tgcaaaaact gcggtgcgga
    11461 tggctgatgc gtacggatca acccgggagg atatccgtgc catgaaatat ttcgcctatg
    11521 ccgtggtgct cattgccctg ctatccggcg cgatggtgct gtcgcaggga cggacggatg
    11581 acgtccgcac gcccgatgcg acccgcgaaa cgaagcccga cgagcccgcc cgcgcgcccg
    11641 atacgcggaa acccgacgcg gacgcccaga agcgtcccgt tgacgatccg ggcgccaccg
    11701 aacagccggc gaaaaaagac gcggacaccg cggcgccctc cgatgacgcg aaaaaaactg
    11761 tcgagcccgg aaaggggccc gacacccgca cggcggagcc ggaaaagaaa gacacgaccg
    11821 agcacattat catcgatcgc aaggaccgtc ccgaggacca tgcgcccgag cggcccgccg
    11881 agaagaagga ccgccgcgac aagacggatg atgacgcccc gtcgcggacg cgtcgtctcg
    11941 gcgacgaggg gaagtttatt ttcggcgcgt atgcgggata cgggttcgtg tatccgagcg
    12001 gcctcatcgc cggcacgggg gaccagggcg ccgacgtgac cgccggctat gccgtgggcg
    12061 gcgagctggg ctacatgctt ctcgaggggg gcggcctgtt tctgggcgtg gagtacgcga
    12121 gcaagcccat ctccatcaac cggacctaca tgcgcctgtc gcagcaagtg cagtaccagc
    12181 tcgccttcat cgattatgtg ctcacctatc gcggtttttt cgacgccttc gatacgcgca
    12241 agagccgcct cttcgccgag atcggcatgt tttacggctc gcggagcggc aggtgggtga
    12301 tgcgcgaaac gatgggaagc accacctatg agcgcgacat ggccacgctc aagcccggca
    12361 aggtgcaaaa cgaatacggc atctgcttcg gctttggcgt gcagttcgac gcggcgcgtg
    12421 acgtgagcgt gaacgtgggt ttgcgcttcg agatatcgct ccagaacgcc ttcgaatacg
    12481 aggacgagct caagaccaac ctgggaatgc tcaccctggg cgcctgctac aagttcgatc
    12541 ctttttagcc ggttggtgta gcagcgggtt tttcgttgcg actatttccc tttgcgtcca
    12601 atgtccggac acaaacgtcg ttgccccctt cgtgcagggg gtttcgtgtt tttgggggtt
    12661 ggggcaatgg cgggtgtgtg taattgggcg aagtggcgaa cagcgtatcg ggcactgaag
    12721 ttgaagctgt ttccgggtcg cattcgaaaa caggtattcg ccagcgctcg gcaaatctag
    12781 cggctggtcg gaattaatta gttcaaccgg ttaatcgtga acggcggtgg ggtcaggggg
    12841 tttgaaagac gcccacggtt tcgtcaaagt ctcttttgct gtgtataacg gccaatattt
    12901 tcacgccatc cggggttgtt ctatacatga tgcgatatgg cggtacaatg agctccctcg
    12961 cggaagcttg catgatttcc ggtatcagtc gtcccatttg cggaaatgca tcgaggatat
    13021 ccgcccttga gacgatcgaa gagacttcta ttcgcgcgta atgaggggac tctgtagcga
    13081 tataatcata gatttttttg aggtcccgtt ttgcggactc ggtccagtct accatgtttg
    13141 gatttcacgt tttaattctt ctgcggtaat gaccctgccg tttcgtatgt catcaaggcc
    13201 cgcatggatt ttatcgatga cgtagaggcg atacatcatg tcttcgatgg ttgcattgtc
    13261 ggggagcttc gagatggcgg ctaaggcttc ctgtttaatt gtttgcatgg gttttacacc
    13321 tcagaggata gtattctgag cgactgtcgg cgtcgatttt ttcacgatag caatttcaat
    13381 aattttattc aaggtttttt tgtccttctc gtcccttatg catccgcttg tgcgaatatg
    13441 agttttttgc aggatgcaga ggatgtaaat ggatcttttt taaccattcc ctgccgtcac
    13501 ggcgcaaccg acagcagctc cgagaccgtc acgaagcgga acccccgtcg ccgaagctcg
    13561 tcgatgatcc cggggagcgc tgcgtgcgtg tgatgcccgc gcccgttcac gtgcatgatg
    13621 gcgatggacc cgaagcgcgc gccgccgagc acgaagcgac ggatcatcgc ggccgagacg
    13681 cgcgggtcgg gatcgcccga gaccacgtcc cattgaatga gccgcagccc cagcgcgccc
    13741 gccgccgcga cgagccgttt gttgtaccag ccgaacggcg cgcgaaacag cgttccctgc
    13801 ctgccggcga tgcggtgcat gatgttctgc gtttcctgga tctcgtatcg cagcgcggcg
    13861 tcgccgaggg cgcgcatgtc cgggtgggaa tacgagtggt tcgcaagttc gaaaaaggga
    13921 acgcgcgcga gctcccgcgt ctcctccgga tgcgagagca tccatcgtcc gcccaggaag
    13981 aacgtggccg gcgtgcgggt ccgcgtgagt atgtcgatga tgcgccggtc gtagccggtc
    14041 ttttcgttga ttcgctcgca ggcgtcgaag gtaagcgcga ccagcggcgt tcgccgtgat
    14101 ccgtgataga ttgcggcggg tttcaccggg gagagggcgg gcgcctgcgc gtgccgatca
    14161 ccgcgctccg ctgggcgcgc ttcggtcggg aggattgcca tacagaacgc tgcgatcgtt
    14221 atcgcacgca ccatgagcac gtatcgcgcg ctcttcgtca tgtccgatgc ctagaagcgg
    14281 ccgcgcggta ttacgcttcc gcggccgcct ttcgccggcc cgtccatatc catccggcga
    14341 tgccgccgat gatgaggagg atgccgatca cctgcgcctg cgacagtccc agcggcccgc
    14401 gcggattgag ccgcacgaac tcgaccagga agcgcggcag cccgttcagg atcatgtaca
    14461 ggaagaagat gcgcccgacg ggccagtcga gcttgcgcag ctgcatgagg agcgcgaaga
    14521 cgataaacga gaagtacacc tcgaagaacg gcgtgggcag caccggcgtg gtggtgggca
    14581 caatgccgtt cgggtagggc gtcgcgatgc cgagcgtcga gacgacgccg tagcagccgt
    14641 cgcccgcgac atggcagccg aagcgcccga agcagtagcc cagcgccatc gacggcgcta
    14701 cggcgtcgcc gacggtgagg aagtcctcgc cgctctttcg gatgatgatg aagcagatga
    14761 tggccgccag cacgaagccc ccgtaggccg agagcccctc gccggagaag atcatcgact
    14821 tcgcgctctg ccagctggac gtgaagtcgg accagtgttc gaagatgtgg aacatcttcg
    14881 agccgacgat gccgccgggg atcgcgccca gcaggatccg ataggcgagc tccggattga
    14941 tgtcccgcag cttgagctcg cgctcgagca gcagaaaggc gagataaaag cccatgccca
    15001 gcatgacgcc gtagccgccg atctgaagga atttatactg gaataagatg ggaaacatga
    15061 ttgcctcgct gtgcgctgtc tgtggttatt tcacgaccat ggggcccatg tcgtccctgc
    15121 cgcccagcac gtggacgtgc aggtgccaga tgacctggcc gccggcggcg ccgttgttga
    15181 tgatcacgcg gtaccccttc gcgtcgacgc cggtgatgcg cgcgacctcc ttgacgccca
    15241 ggaacaggtc ggccatgatg ccgctgttct cggcggtgag ctcgtcgagg tttgtggtgt
    15301 gcgtcttgtg caccaccaac gcgtgcaccg gcgcctgcgg gctgatgtcc gcgaacgcga
    15361 acacgcgctc gttctcgaag atcttcgtcg aggggatctc ccccgcgatg atgttgcaga
    15421 acaggcatcc catggcgcgc tccaaaaccg atttattgtt gattgttccg ccggcgccgg
    15481 cggatcgtgt ttgcaggtac cgtacgcgat actattcgtc aagaacatta ttgcgggaat
    15541 cggagcgggg cgcccgcgcg catggggcgg gaagcgggac ggtgaggcat cacgcatgaa
    15601 aaccatcgac gacattcggg ccatcgtgta cgatcccgcc gaggagcgcg cgccgctcgt
    15661 cgcgcgtttt ctcgcgctcc cgggaaagcg gcatctcgcc tgtacggacc gcgaatcggt
    15721 tacgcgcgcc ctcgacgggc tgcgggcgga agggctcgac tcgaaggagg cgctcgtcct
    15781 ccagcgtttc atggggcgct tcttccagaa gtgtcccggc tcgccgcggg tgatctgctg
    15841 caactatcat ctcatcaaca cctgctttaa ctgcttgtac gactgcgcct attgctacct
    15901 gaacacctac ctgaactcct tcggggtggt gcagttcacg aacatactcg atgtcgccga
    15961 cgagatcgag gcgcacgtgg ccgcgcggcc agcgggggcg gtgctgcgca tcgggaccgg
    16021 cgagttcacc gattcgctga tgatggacga ggtgaccggg atcgcggcgt cgcttatcgc
    16081 gcgcatgagc cgcttcccca acgtgttcct ggagctcaag acgaagtcgg acaacgtggg
    16141 gcacctgctc gcgctcccgg agaagggcaa tgcggtgctc gcctggacgc tcaacaccga
    16201 gcggaacatc gcgttatacg aaaacggaac cgccgcgctg gaccggcgca tcggggccgc
    16261 gcgcgcggcc tgcgcggccg gctaccggat cgcgtttcac ttcgatccga tgatcgcagg
    16321 cgagggctgg gaggcggagt accgcgcggt ggccgcgcgg gccctcgcgg cggtggaccc
    16381 ggcgcgcgtg gcgtgggtgt cgctgggggg ctttcggtac gcgtcgggct ttcgcgagat
    16441 catccgagcg cgcttccccg acgaggcgct cacgcgcgcg gagatgtttc ccggcatcga
    16501 cgggaagtat cgctatctgc gcccgcggcg gctggcgatg taccgcgcga tgcgcgagtg
    16561 catcaacgag ctcgccccgg acgctttcgt ctacctgtgc atggagggcg cggacatgtg
    16621 ggaggatttc gccggggcgc gctacgcctc ctccgatgat ctggaggagg acatcgcgcg
    16681 ccacctgcgc cggaccgtgc tgcgctagtc gccggtcacg atcaccgtga ggctctcgta
    16741 gatgagccag ttgaaatacg gaaggcgcag catcacgcgc tcgtagtcga ccgagtggac
    16801 gcgcgtgatc ttcgcctcgt cggcggcctt gcggatgctg ctgtcgcccc aggccaccag
    16861 gcccaggacg ccctgcgatg acgagcggcc ggtgcgcacc gaatcggtgg tcgcctggcg
    16921 ggccttctcg tccgcggccg cgtccttcgc gtcgaccggc ttggtttctt tctcgctttc
    16981 tttgacggcg gatttcgccg tatcgtccgt tgtgtcggcg ggctttttgc cgtcggcggg
    17041 agcgtcgcgt gcggctttat cggacggcgc tgcggcgtcc cgcgcggcgt cattcgcgcc
    17101 tttcgtgtcc ccgtcggggg ccgggggaac gccgtcggat tttttcgacg ggccttcgtc
    17161 ctttttatcg gcgggagcgg attcgcgacg gggtacggca tcatcacccg tgacggcgcc
    17221 ggtgtcggtt ccgcgtttga tcgacgccga tgtggccgaa aggacatggt cggtggccgc
    17281 gatcgatccc ttgttcacgg tcacgtaaaa gccctcgggc ggcggcgtcc ggggaatggt
    17341 gtagcgggtg attgacgaaa cgcatcccgc gcaggttgcg agggcggcga tcacgacggc
    17401 gagcgcacgc atgctattca ccgtagacga ccgtcgtgta ggtcatgtac acgagcagta
    17461 tgttgaagtc ctcgtagtcg acatgatgga tcttggtgat cccggcgctc tcgcaggcct
    17521 tcttgatgct gctgtcgccc cacgcgacga tgcccaggac cgacgaggcc gtggcgcgtc
    17581 ccaccttcgg gctgttcagg ttcgcggtca cggtgaaggg tccctgcacc ttcgtataga
    17641 tgcagccctt gggggtcggc gtggacgagc agccaaggac gccgatgacg aggacggcgg
    17701 cgcacataac ggcgatcggt ttcattctca tggttctcgt gtctcctttt ttatgatggc
    17761 gtgacgggga aaacgcattt cgaacagtat acgcaatgat gaggggatcg gcaagttttt
    17821 tttcgatgac gccgatggcg tgtgcggcta ccagacgtac ttgaccacgc ggttcccgca
    17881 gagctcgccc acgagcagcc ggtcggtgtt cgcgatgcgc tcggtgagcg tgcgcaccat
    17941 cttcacggcg tagcgggggt tcttggtgat gaacgcgacc agctcctccg gcgagtcgat
    18001 gaatgtggcc cgcacgtcgg tgagcgcgta ggcgttcgcg gtccgctcga tctcctggaa
    18061 gagcgacacc tcgccgaaaa aatcgccctt gccgaggcgc gcgaccgata ccttttcgtc
    18121 gttcaccgtg aaggaaatct cgacctcgcc ctcgaggatc acgtacatgc gctgttcgta
    18181 ttttccgctc ttgatgatgc gcttgccttt cgagaagctc gctatccgtt tgcccatgga
    18241 ccctcgcagc cggtgatggt tatactatac caatcggccg tccgcgttct ccggtccaca
    18301 actatcgtga aaaacctgtt gattttttcc cgcgtgcggg gcggtatgag tggcgtcccg
    18361 gctctcatat gcggcgaacg ggcgggagcc gtgcgccgaa accgacggta cgtgtcgcgg
    18421 tgtcgttcat atcatccctg gcggtgcgag tatggataaa tcggaaacgg cggtgcaaac
    18481 cttcggcgcg ggcttcaatt gcgcccaggc ggtcctcggc gcgttcgcgt cggaggccgg
    18541 tctcgatgcg gacacggcgt acgcgatcgc cacgggcttt ggtgcgggcc tcgcctatcg
    18601 gggggaaacc tgcggggcgg tctcgggcgc ggtcatggtg ttgggactgc ggtacggatg
    18661 gcggggcggc gatatcgccg aatcgcgcga ccggacggtc gagcgcgtcc gggagttcat
    18721 ccgccggttc gaggggacgc acgggaccat cctctgccag gaccttctgg ggatggacgt
    18781 ggccgatccg gctgcgctct gcaaggcgcg cgagcggaac ctctttcgca cgctgtgtcc
    18841 ccgctatgtc cgcgacgccg cggcgatcct cgaggacatg ctcggcgcgc cgtgatcgcg
    18901 catgccgaca tcacggccct cttgcccggc ggcgagggcg actggatcgt cggtttcgcc
    18961 gatctgggcg gcctcctgca cgaacgcttt gcggggcatc ccttcgccat tgtcatcggc
    19021 cgtcggctgg acgatgccat catggacgat gtcgcggacg ggccggtgcg ggcgtattac
    19081 gaccactatc gcgacatcaa ccgcaggctg tcggatgccg ttcgcgcggt cgcggatcga
    19141 gtgcggaggg acggattcga ctgccgcagc gtcgaaccaa cggtgctcga cgagcaggtg
    19201 gcggcggact acatggaaac gatgcggctc gatttttccc acaagatggc ggcgacgcgc
    19261 gcgggactcg gctggatcgg caaaacggac cttttcgtct cggagcggtt cggtccgcgc
    19321 ctgcgcctgg ccacggtgct cgcggaccgg gaggtcgcgc ccgcgggaac gcccgttacg
    19381 gtgagccggt gcggcgcgtg cgcgctctgc gtggagcgct gcccggcgaa ggccgcgacg
    19441 ggaaagctat ggcgcataac ggttgaccgc gacgagtttt acgatccctg gaaatgccgc
    19501 gagaaatgcc gggagctcgc ccggacgcgc atcggcgtga atgagagcat ctgcgggatc
    19561 tgcgtgtcgg tgtgcccggt cggacgcgcc ggcgccggcg ctatctgacc ttcgacgcgt
    19621 cccactgttt ctggatgccg gaaacgcggt aagacccgcg aatgctgcgg ccgccctgcg
    19681 tgtattcccc gctgtattcc tgcacgaacg gcccgttcgc cccggtccgt ttcacctcat
    19741 ccggcgtggt cacggagcga acgaagaaaa tcttccctcc cgaaatgcgg acccctttga
    19801 gcggttcggt cgcaccccgg ccccagttgg gaaagcggat cgagccatag aacgcgccat
    19861 tcgcgcgtcg gagcgccagg atgccgctgt tcccggcggc gttgatggag taggttccgg
    19921 tgtactcgtc cggcggcgcc tgatgggccg gtccggccgc tgtttcaaca gggggctgga
    19981 tgaactcgat gtattcgatg cggtgcaggc cgatcgcatc gaggttcgtg cgttccatgc
    20041 ggctgtcgag cgcggcgcgt ttcgttttgt gcgatgccgc atcgcggcgg cagacgatga
    20101 gcggtcccgc gaccaggacg gcgcgcgccc ggtagaaatc gccgcttcgt tccttgacga
    20161 gcgcgccggg ccccgccagg gcggaccaga ggagcgtcgc ggggagcgcc atcatcatcc
    20221 ccccgatgac gagcaggacg ccggtcgcga tgcgtttgtt tcgagcggtc ggattcatgg
    20281 cttcctccat ggttcggggc ggtctagggc ggtgagtcgc tcaccccttc tttacgataa
    20341 gcagcgtcac atcgtcggcg ggcatgtagt cccgcatttc gtccaggatg ccccgcttga
    20401 tttcctccgg ttccttttca cctaacgtca acaggcagct cttcagtcgc tcgtcgccga
    20461 acatgtcgct ctcgggatcg cgctcgtcct ttcgcgcgcc gcgccgccac gactcggtga
    20521 tgccgtcggt gtacagaaac atcacgtcgc cgcgtgaaag cgtgagttcg ctgtcatgaa
    20581 gcatgccgtt gatgtcttcc acgagaccga tccagatgcc gtcggtctcg acgagctcga
    20641 ccgttttggt cgcggcgcga tagatcatga tgtcctgatg cagtcccgaa aagtagaacg
    20701 tgccgttctt gaggcaggcc aggaccgtga tggtcatgta cttgtcctcg ttgagcttct
    20761 tgatgttggc ggtgaccacc tggttgatgt ggctgaggag ccgctgcggc ttgaggttcg
    20821 gctttcccgc gagggcggtg tggatggcgg tctgcaccat catcatgatg aggccggcgg
    20881 ggacgccgtg cccggagacg tcgccgatca ccagccagtc gagcccgtcg acgttgatga
    20941 tgtcgtaata gtcgccgccc acctcgtcgg ccggttgcat ataggcggcg atctgaaagc
    21001 cttcgatctc cggccgatcg gggaggagca ccgtctggat cttcttggcg agctgcatct
    21061 ccccccagag ctggtcgcgt gcgctcttga gctcggcggt gcgctcctcg accttgcgtt
    21121 ccaggtcgcg gttcatctcg tccttgatgc ggtccgtctc cttgagctgc tcgaccagct
    21181 tctcctgggc ggcgagcatc tcgcggtggg actcgagcgc ctcctgctgg gcgagatacc
    21241 gttcgcggcg catggtgttg atgcggtcgg cgagcccgag ggagagcagg accacctgca
    21301 tggccgagcc gatctggatc gaataggtgg taaggaagaa cgtggggacc gcgccgaggt
    21361 tgcgcaggcc cagcagcatc acgccgatta gcagcgcgag ccaggcgatg aggtagtatt
    21421 tcgccgcggg aacgcccttg cgcatgcaga tgatcccggc cgcgatcgaa gggatggcga
    21481 gcacggtggc catgagggcc acgaacttca ccccgagcgc atagggcgcg aagatgctca
    21541 tgcccatgcc gaggaccgag cacccgagga ggacgagcag gaccttgtcg atcgccggga
    21601 cgttcgcctt cgtgttgagg tagtcgcggc agaacaggat cgagaagaat atcgacagga
    21661 agagcgagaa aagcacgctc acgttggccc accaggggac ggcgaaccac aggtattcga
    21721 aggcgaagcc gttgagcgac atctgcccca ggatgaacgc cccgatgaat agaatgtaga
    21781 tcaggtatac ccgctcgcgg atcccgatga acagaaaaag attgtagagc agcatgacga
    21841 tcatgatgcc gtagtagacg ccaaacccgt attgcgagcg gtttaccgtt tcggcgaagg
    21901 ccgtgggctc ccagagcgtg agcggcacct ggaacgagct ctcggtctgg aagcgcaggt
    21961 agaccgtcat cgacgagccg cgcggtatgg cgaggcggaa gagaaacgtg tggtagcgca
    22021 cctcgcgttc gttaaaggcg acgcggtccc cggccgtgcg catctcgtat ttccccgatg
    22081 gggaggggga atagaaccgg atgtaatcgt gatgggggta tcccacttcg aggagccagt
    22141 tctggatcgc gggatggttg tttttcacgg tcacgcgcat ccagtaggcc gacttcgtga
    22201 agccgaagtt catgaccggc gcggtgttgc ggacgaagcg ttcgtcgtgc gtccccgcgc
    22261 ggatgtcgtc gaacgcgatc gttccgtcgc ggtcctccag gtattcgacg gacgggccca
    22321 gggcgtattc gcgggccgcc tcggtgagaa cgacggccgg cgcggcatgg gccgcggtgg
    22381 ccagcgctcc ggcgagaagg atggttccga gcctcacgat tggttgtgtt gttttcattc
    22441 gccccgttcc tcgttgcgtg tccgtcccgg tccactacgt agccatcgac tgtatttccg
    22501 tatcgtgcgt gccgctatgc tgatcgtttc acaacaagca gggtcacatc gtcggcgggc
    22561 atgtagtcgc gcatcgcgtc caggatgccc tgcttgattt cctccggttc cttttcaccc
    22621 agcgtcagca ggcagctctt cagccgctcg tcgccgaaca tgtcgctctc gggatcgcgc
    22681 tcgtcctttc gcgcgccgcg ccgccacgac tcggtgatgc cgtcggtgta cagaaacatc
    22741 acgtcgccgc gtgaaagcgt gagttcgctg tcatgaagca tgccgttgat gtcttccacg
    22801 agaccgatcc agatgccgtc ggtctcgacg agctcgaccg ttttggtcgc ggcgcgatag
    22861 atcatgatgt cctgatgcag tcccgaaaag tagaacgtgc cgttcttgag gcaggccagg
    22921 accgtgatgg tcatgtactt gtcctcgttg agcttcttga tgttggcggt gaccacctgg
    22981 ttgatgtggc tgaggagccg ctgcggcttg aggttcggct ttcccgcgag ggcggtgtgg
    23041 atggcggtct gcaccatcat catgatgagg ccggcgggga cgccgtgccc ggagacgtcg
    23101 ccgatcacca gccagtcgag cccgtcgacg ttgatgatgt cgtaatagtc gccgcccacc
    23161 tcgtcggccg gttgcatata ggcggcgatc tgaaagcctt cgatctccgg ccgatcgggg
    23221 aggagcaccg tctggatctt cttggcgagc tgcatctccc cccagagctg gtcgcgcgcg
    23281 ctcttgagct cggcggtgcg ctcctcgacc ttcgtttcca gggtctgggc gtactcctcc
    23341 agctttttct tggagtcgcg gatcgagccc accatgttat taaaggcgtg cgtgatgaac
    23401 ccgatctcgt cctcgaagcg gacgggaacg gtgacatcga ggtcgccgtt gttcacgagt
    23461 cgcacgccgc gcgagagcgt gcggagcggg gcgaccagcg cgcccatgaa gaagtattgg
    23521 aatccaaagc gtacgatgac caggagcgcc gcgagaatga tgataagcgt gagcgcgaac
    23581 gggtggatga attcgcggta ggcgcggtac gaatagccga cctcatgcac ggcgttggct
    23641 gccggatcga attggacgta ggcgatataa tggtgctggc cggtcgggtc ggtccggtac
    23701 aaccgcacgc cctcgccgtt catcggttcg agcagcgaca acgcatcgcg gcggagttcc
    23761 gcatcggttc gcgacgcgtc gttcatgagg gccatgagcg ccgcgcggaa cggctcgaag
    23821 tcgccttcct ttttactcgc gaatttcacg agcgattcgc gaaatccctt ttccggtaat
    23881 gcgcggatct tgttggcgcg gtaggcgata atgcgcttcg cgccgtcgag tcgcgcaagc
    23941 aggcggcggg ccggccgcgt ctcttcgcgc ggcatgcgtc caagctcgtt ctggatgaag
    24001 gcgcgatatc cggcgaaggc cgccggcgtt tcgtcgagaa gtttcatgat gcgtgactgg
    24061 aagccgtccg tcggcaggga gcgtattttc ccgagcagcg cggcgttgat gaattcctgg
    24121 cggtaggatg cgtggtccac cgccgcgccg ccgccgggag cgcgctcgat gccgatgaag
    24181 cggttcgtgt caagggcata ggtcgcccga taggagaaat cgtcggggcg atagtcgctg
    24241 atgagcacgc gcgcggtgag gtgctggtgg gcgaagtcgt atccgcggtc cttgtcggtc
    24301 agggcgaaat aggcgatgaa ctggacgacc agcaggaagg cgacgccggt gatggtgatg
    24361 agcttgccga tgaacgaggt ccggtcgcgc gtgctgttca gatagatgat cgcggccgcg
    24421 gcgaagcaga gcacgttgaa gatcacccac gaaacctgat agagctcgcg gtccatcgcc
    24481 ccatcgcggc tcagggtgtt ggtgactccc ggggccaggc ccgatacgaa caggaagatc
    24541 cccatggtga gcgatatcca gcggtcgcgg cttttcgtgg tgacggtgcg ccacagcgcg
    24601 agcggcagct ggatgaagat gtagagcagc atgagaaggg aaaagtattt gctgaaggtg
    24661 tcggcgtcga agtcccagta gtgggcgttg atctggtata cggtccggcc gtgaatggtc
    24721 gaagcgatga acgcgagcga cacgagcgcc gcgacggcgt atgaaacaat gaaatagatc
    24781 ctgccgaagc gcggggcgcg ctcatcggga aagtaaaagt ggatcatgtt gaaatgggtg
    24841 atgatgaaca ggaccgatcc aacggtgaac catcgatgat aggcggcaag cggatgatag
    24901 aagctgttgg ccgcgaaata cgcgaggttg aacacgaccc agagggcgta tcccatcgcc
    24961 aggtggaagg tcgcttttga gcggttgcga attgaaaaaa gaaaaatcgc aagaaacaaa
    25021 tggaagagca ccgggatgag catgcccacc gaaaagtaat tcaggtaaaa cgtcgaaaaa
    25081 aatcccatgc gaaccctccc gccccgtcga acgaacgggg ctggcagatc tgattacttt
    25141 tgtgtgtttt cactcggcga aacgccgatg tctcagacaa ccaggaatag tatgatgcgg
    25201 tcgctgataa aagcaagtac tttatcggaa gcggatcgcc tcaggcgcta caacgcgccc
    25261 gcgccgcctc gacgcgtttc gcgcatttct cgaagccgat gagcgggagc gcctggtcga
    25321 gctcggggcc ggcaaggcga cccgtgaaga tcgcgcgcat cgacaggaag agcttcttgc
    25381 ccttgacgcc ggatcccttg atcgcggcga tgagcgccgg cgcgaaatcc gcgccggcgc
    25441 ccgatgcggc gatcgcatcc tgtgcggcgg cgatcaccct ccgcgcctca tcgtcgtcga
    25501 ggaaggcggc ggcatcgtcg tcgagcgccg ggacctcctc gagaaagacg ccgatgatcc
    25561 tcccgatgtc ggagaggacc tcgcaataga cgcgcgtgag cgcgatcacg gattcgagcc
    25621 atgcgcggtc gagcccggcg acggcgtacc cggccttctc gatatggggg acgaagagct
    25681 cggtgatggc gccgatcccg gcgctgcgga tgtagtgcgc gttcatccag cgcagcttct
    25741 ggaagtcgaa gaccgccgcg ctcttcgcga ggcccccgat ctcgatgtgc cttacgatgt
    25801 cgtccaacgg catgatctcc tcgccgctct ccgccgccca gcccagcatg gcgatgtagt
    25861 tcaccagcgc ctcggggagg tagccgtcgc tccggtagag gtccacggag gtgatgccgt
    25921 ggcgcttgga gagcttcttg cggtcgtcgc ccatcaccag ggccaggtgc gcgtactcgg
    25981 gcacggggag gcccagcgcg cgcgcgatca ggatctgctt gggggtgttc gagagatggt
    26041 cctcgccgcg gatcacgtgg gtgatcgcca tgagcgcgtc gtcgatgatc acgatgtagt
    26101 tgtacaccgg cacgccgtcg gagcgcacga tgatgaaatc gcccccgatg ttctcgctgg
    26161 agaaggcgac cggccccttg atgaggtcgt tcaccgtgac ggattcgccc gcgggaacgc
    26221 ggaatcgcac ggtgggcttc aggcccttcg cctcgcgctc ggcgcgctcg gccgccgtga
    26281 gatcgcggca tgtcccggga tactcgaagg cgccttcgcg cccctcgcgg cgcatcgcgt
    26341 cgagctcgtc ctgcgtgcag tagcacgggt aggcgctccc ggacgcgatg agcttctcgg
    26401 tgtactcgcg gtaaatgtcg aaccgctcgg actggcggta cgggccgtgc gcgccgccgg
    26461 tatcggggcc ctcgtcccac tcgatgccca gccagcgcag gtcggcgagg atcgacgcct
    26521 ccgattcgcg ggagctgcgc tccatgtcgg tgtcctccac gcgcagcacc agcgtcgcgc
    26581 cggtcttgcg cttgatgagg tagttcagga tggccgtgcg ggcgttcccg atatgcaggt
    26641 agccggtggg gctgggggcg aatctgaggc gcatgactat tctcctggcg tatgcgggtc
    26701 gttcatgatt gtcaatggtt tcatcacgct ggcggatatc accgtcgctc cgccgcaaag
    26761 caaacgtcct gtttctgcgg ctcccggcga ccctctcggt cgtctgctca cggtgacacc
    26821 cgccagcgtg atgaaagaga aggtgaggca cagttgatga atactgctac cgtatgggta
    26881 tacggggata cggtcgtcaa caatgatttc cccggccggc ggccatgttt cggaaaggta
    26941 aaatagtcaa aatcggccat aatcgcgggc aatatacttg acaagcgcca ttcgatggcg
    27001 caccacgcga atgtcgacat acatcagcct ccgggagcgc atcatggaca gtgccagcga
    27061 aaagacgacc tccaacttca tcaaggacat catcaacgag gaccgaaaga acggcaagca
    27121 cggcgcgcgc gtgcacacgc gcttcccccc ggagcccaac ggctacctgc acatcggcca
    27181 cgccaaatcg atctgcctca acttcgggct cgcgaaggag tacggcggcc tgtgcaacct
    27241 gcgcatggac gacacgaatc cctccaagga ggacgtggag tacgtcgatt ccatcatggc
    27301 ggacgtgcgc tggctgggct tcgactggga cga
//
LOCUS       DAORHF010000020        27249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1087_length_27249_cov_6.373171, whole genome shotgun sequence.
ACCESSION   DAORHF010000020 DAORHF010000000
VERSION     DAORHF010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27249
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1087_length_27249_cov_6.373171"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PKJ16_03185"
     CDS             complement(<1..331)
                     /locus_tag="PKJ16_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46019.1"
                     /translation="MIAYEIGAVEAGYRGTNVLEGISVRIHADDFLGVIGPNGAGKST
                     LLKLLTGNLRPSAGTIAFRGRALAAYTERELARELAVVHQHIENVLPFTVEEFVTMGR
                     FPHRRPWE"
     gene            complement(328..1302)
                     /locus_tag="PKJ16_03190"
     CDS             complement(328..1302)
                     /locus_tag="PKJ16_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HNV46020.1"
                     /translation="MKRSLAVIGIIAALAAVFALCISLGATFIPPHTALAALIGGGDS
                     TERLIIATLRVPRMLMAFAVGAALSVSGLLYQALFRNPLADPYTIGTAGGAALGATVA
                     ICLSLGAGAVTAAAFAGSLAAVIAVNALAARRGFSSVSLIVAGIALSFILSSAVMLVF
                     SVARPQLVHKALMWLMGDLSIARYALLPPLGALVALLIAGAMVFHRRLDILSFGPAFA
                     RALGVSPAAVKNIFWLSALLAALAVTLAGVIGFVGLIVPHIMRRSLGPAHRLLIPASA
                     LGGGCLLALCDALGRSILPLSEVPAGVMTGFFGGMFFLVLFATRGDAR"
     gene            complement(1292..2167)
                     /locus_tag="PKJ16_03195"
     CDS             complement(1292..2167)
                     /locus_tag="PKJ16_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HNV46021.1"
                     /translation="MKHAIAARLPLLAALLVFGALYAIADTGARPAPAPRAHGRIVSL
                     SPSITRQIMELGAADAIVGVTTYHPALGRPVAPVGDLSRPSIERIVALAPDVVMYSRE
                     DADTQFTELLAATGLPVFVTGANRSFEELCDNFRSLGRLLGRGAIAEERIAAVRARIA
                     AIKRPARRLRIAVLLSHVPLVAASGQSFINGIIESAGGENVFRASSIPYPVITLEHLV
                     AARPDVLISFTDNPEPLFPDAHGALTAIPAVRRGAMVTLDHSHITQYTPDGYADAASA
                     LASILTSLERRIDET"
     gene            complement(2168..2332)
                     /locus_tag="PKJ16_03200"
     CDS             complement(2168..2332)
                     /locus_tag="PKJ16_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46022.1"
                     /translation="MKLTKPERDILAGAHGSALQKALASVVRFGDVFGAVRQGQRVDI
                     AENGTVTLRG"
     gene            2480..3133
                     /locus_tag="PKJ16_03205"
     CDS             2480..3133
                     /locus_tag="PKJ16_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HNV46023.1"
                     /translation="MKRALRIALSLVLVSVAALVGINIYVLAATAPFIFDEAKDVPRA
                     YAVLVPGAFAYRDGRLSHVLYDRVQRAQDLYRAGTVKRFLLSGDHGRKTYNEVNIMKS
                     HLMKRGVPGADIFTDHAGFITYDSIVRAKRVFQVRDMVVVTQRYHLPRAVYIARTNGI
                     DAYGLAADRRTYRAILWYRVREFVAVGKAFVWLLIDKRPRFLGTPIPITGDSAKSWD"
     gene            3189..4532
                     /locus_tag="PKJ16_03210"
     CDS             3189..4532
                     /locus_tag="PKJ16_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013886653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV46024.1"
                     /translation="MERLRYYADMLRGPAFTAHLARSWAVGWPMVFIMALEFLINLTD
                     VFIAGRLGKEVQAAVGFAMQLYFIFIVAANAITVGTVSVVSRIHAAGDGEGVRAAAWS
                     VIATCLGAGAALGVLGYALSPVVIGMVNLPAEVKRYAAPLVQVLALGLPFHYFNINAS
                     GIMRATDRVKRALATVMATCVINIGLNFLLVFHTPLGFLGIMVSTVGATVVGSLINAG
                     FMAQILRAPRVFSRGLLGRILLVGWPSGVQQVMWHVGAAALFLILGALPNHNVEVIAA
                     YTNGLRIEAAIFLSAFAFNMANAVIVGNFLGAHRQRDAFRGGLVTAGIGVAVVAALTI
                     VVVVHAKPFAGFLSANAMVIDRCATYLYISMLSEPFMAWGVILAGALGGAGDTRGVMM
                     IIVGSQWLVRLPLAFVLGIVLGLGEAAVWWSMNASIFVYCILLTRRYFKRKWLHL"
     gene            4529..5383
                     /locus_tag="PKJ16_03215"
     CDS             4529..5383
                     /locus_tag="PKJ16_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HNV46025.1"
                     /translation="MKREPLDLSHRELLAGRLRAVDTALSEYSFANLYLFRDNHRYEV
                     LFDGDDVFVAGLTYDGARFIMPARNVDPACVERLAALLADFDIIFPVPEPWLPLFPAP
                     RFAHAYSDGDTDYVYTIEKMSTYPGKKLHKKRNLMKQYEELYEHEEFPLTDDRIADAR
                     AVLEEWQTDMGVPPRETDYRSCVEALERYEDLALCGGIYYAAGQPAGFILGEELDAKT
                     FALHFAKGLRRFKGVYQFMYNSFARIMPAQYCCLNFEQDLGREALRQAKTSYVPDQLL
                     RKYRVRLR"
     gene            complement(5417..6484)
                     /gene="rsgA"
                     /locus_tag="PKJ16_03220"
     CDS             complement(5417..6484)
                     /gene="rsgA"
                     /locus_tag="PKJ16_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNV46026.1"
                     /translation="MSHDNLARLGWGPDFERFYSEHKTEDSHPARVISVSRGTWLLAG
                     VQGERFGVPAGKFHYHDIEMPVVGDWVVARGTGDDSWLVIQDVLPRRNHLSRKAPGKT
                     SARQVMAANIDIMFVVQDALALNCALAERYLVLAKGSDITPVLVLNKCDTEAHAAEAL
                     GAIAERIPGVTAVAASALTGLGIGDIRAHVAQGVTACLLGPSGAGKSSIINGMLGTQR
                     QRIGEIREGDRKGRHTTTVRELFFLSGGGMVIDTPGMRELMPWDASGLDDSFRDIEEL
                     AAQCRFDDCAHETEPGCAVRQAVESGTLDPGRLENFHRLTRELAYLESQIDENARREK
                     KRKEKELHRLIKDYNRNRRKP"
     gene            complement(6771..7301)
                     /locus_tag="PKJ16_03225"
     CDS             complement(6771..7301)
                     /locus_tag="PKJ16_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNV46027.1"
                     /translation="MASREERKQKVVTVLNTARSMELQAIHQYMNQHYNLDNLDYGEL
                     ARNIKLIAIDEMRHAEMFAERIKELGGEPTSDLAGGVEKAQSVDAIFPFDAKAEDDAI
                     DAYNRFLAVCLENGDSITAKLFETIIDEEQKHLNYFDNVNEHLGKLGAAYLARIAGTS
                     ASTGPASKGFVLGGGE"
     gene            7467..8456
                     /locus_tag="PKJ16_03230"
     CDS             7467..8456
                     /locus_tag="PKJ16_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV46028.1"
                     /translation="MDTAITTPERLQELINAYRTSRIILTAFELGVFTAVGTGDADAA
                     RVARAVGADERGTDRLMNALCAIGLLEKRGGRFFNTPFGAERLVRGAPRYLEGIRHSV
                     NLWDTWHTLTDAVRAGHTVVNGDPACRDPNWFEGFIAAMHQRASAQAPRMIECMDLDG
                     VMRAIDIGGGSGAYAIALARAREGLRCTVFDLPNVIELARGYVGRAGMLERIDFVAGD
                     FNADDFGVGYDMALLSAIIHMNSPDGNRRLFAKIAACLNPGGRLVVQDFVMNDDRTGP
                     AVGALFALNMLVGTSAGDTYTAAEIESWMREAGCARTEARDTPFDASLVIGWK"
     gene            complement(8537..9097)
                     /locus_tag="PKJ16_03235"
     CDS             complement(8537..9097)
                     /locus_tag="PKJ16_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV46029.1"
                     /translation="MELTQAILKRRSIRKFTDYVVTDAEIAELLEAARWAPSWANTQC
                     CEFIVVRDRGLIEKITAAYSEFNPARKCSLAASALIIGCAHSKKAGVKEGGWATKFSS
                     WYMFDLGLAVQTICLRAHELGLGTVIVGLMNHDICKNLLDLPEDYEVVVSIPIGKPAV
                     EAKEGPARRAVAEFTHLDRFGNPFMS"
     gene            complement(9288..12233)
                     /locus_tag="PKJ16_03240"
     CDS             complement(9288..12233)
                     /locus_tag="PKJ16_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSEP-CTERM sorting domain-containing protein"
                     /protein_id="HNV46030.1"
                     /translation="MIANTAARIEALIAVVRRPINWIIFGTVPQALLLVANLRSYSLV
                     AGELDPHQNRIALSLLIAEIALLALVAVLGALFGARRREIPWAAGLPALFAAAAYLYL
                     FTETLPAAFPRNVPEWILSYDGVVYAQFIAVMPAAFLALATAALFRTTLRFPQEVAVW
                     SVSLAGPPVFWYLFVLLMQSSLSRNWSGAAVAAVLLPFFIATTALMFLAIIRLFALFY
                     ARFYRENRWLSLAAHAAFGIAGPIGGLLLNRAIPFPTDFQSPAVYALAVANGIVLMLP
                     PARSVRLNRAIFLTRAAMFPFTLYFFAVFLPFLPLGIPAILAAGAGFLILTPTVLFLL
                     HGSRLVDDYRADIARGAPRATRIMLAGALLVLPIAYTARALVDRVNIHAALDYVYEPD
                     YEKDRVFSGSRFLLEHSLANLQETKKGVQMPFLTGYYNWIVFDNLVLPDEKMDRLHRF
                     FFGGPMAPYGKGNVLGDIFGRRTSVAREGGRVPLPLPGRVELVSVAMRHAPAGDAVKR
                     TVVITMKNRTKLQGEYATTVTLPVGTLVTGYWLHIGKERVPGRIFEKKTALWVYRMIK
                     SVRRDPGILYFTGEQELALRVFPFGPHEERVTEIELTVPPGGGGAATIGGRRIDLGAA
                     LSDTANIVPATGATGVLLPPAAKRAMLPVTRAPYLHLVLDRSDATGLDAARALAAARA
                     VLERFPRIARCALSAANFESVDILPPTSSRDRIASITAGAIDSALTRRGGFSAQREVK
                     RILTSHRDPESAPIIVVIGRTEAAPDATRTPTREEEDNLSFFGGLAPDIDGYFMTENG
                     SALAPVPFDRKRARAVSPLPRPVHLVRCGARTVPISTDGASELIRCDGAAAMDSIAYL
                     DASTRAYRPLRTGPSEAAVGLAGIVDYRTLKLIPARTRALYPALVATAKRTGILNGAA
                     SYIVVESPSQWRILEEKEKQKLRNRETLEITERADEPHPALVVACALALIIILERFRK
                     RRRNAALYS"
     gene            complement(12223..12792)
                     /locus_tag="PKJ16_03245"
     CDS             complement(12223..12792)
                     /locus_tag="PKJ16_03245"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HNV46031.1"
                     /translation="MNQPTDTIPAAPGLRHSRDMRGRTAIAAVMAASIGLGAISLAPA
                     ELVHAVFLHPAARIAALYLGLPCVEDPGAGYIIPHPALRLAVVPECSGFTFYTILCSF
                     LLFQAFRRFDARRAVPAAIGIVLAALPAALLANALRLVACLYAFIIMPPGTPRAIANT
                     AHLWIGVLVFVPALVLAYYLFERRFVHDR"
     gene            12895..15855
                     /locus_tag="PKJ16_03250"
     CDS             12895..15855
                     /locus_tag="PKJ16_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46032.1"
                     /translation="MPWNPSQALPFDSIGEIVADPLVSCDETGVIEACNAAFALFVGA
                     DRASVPGRGLVDFLPDNLVDAIVASYAPDAGEMEVTLAGHAPRAVSYRRAFISDGDGN
                     RTILCRFTDITKQKWEREQIRMFNYAVENSPASIVITDRNGTIEYVNRKFTEVTGYAM
                     NEAVGQNPRILKSGRQHADYYREMWETILAGRSWYGEFCNVRKDGSLFWEIASIGPVI
                     GIAGEISHFIAVKENITYLKEIEEGLRASDSRTSAILNAVPDLMFRVDTDGRLLDTEV
                     AAKVQEIFPDAIAETARPVIARALEDFDVKIFEYPVYFNKKLQYYEARFIAAGDDEVI
                     AIIRNISDRVAAEEQLKKARDDAEAANRAKGEFLANMSHEIRTPLNSILGFIELLKLE
                     NITGTQAEYLGIVSESAASLLGIINDILDFSKIESGRFEIEAVPFNPRREFESVIDLF
                     TVKAQEKRIEFLSFVDPALPPSVAGDPLRIKQVLANLLSNAVKFTPAGKTISVEVLRV
                     GDADGAARIRFTVADTGIGIPERKRDIIFSAFSQVDSSVTRRFGGTGLGLSISSNLVR
                     MMGGELALESEPGKGSSFFFELALPHAAAEESESLPEIPPSISAVVLGPDGPPGPRES
                     LVARYLTSLRFTWRRITPTDPEHEREPDVFVVFGEQELDGFVARVRGERPEVPAVAVV
                     GSMGEIDTLPRLHARDRVVSLPLGASKLYNALADAVGIGAGAGAIGRAASARALPALR
                     VHALVAEDNAVNQKLMLLMLKQFGATADIASNGLDAFDRYRKGRYDVVFMDMHMPVTD
                     GVEAARMILDYEKVYRLQHVPIVALTAKMMRGGADELLAMGMDEYLPKPVSLAALEAV
                     LVKFCASRDGAEAAEAAPVADAAGAYSFDDSAAELGIGVDALREIVREFMADADETMA
                     AIERGIADGSHEAVYQAAHRLKGAAANLRFRRLADALRALEAAARAGDAGRYRALVDG
                     VINEFGSVRNDISK"
     gene            15839..16624
                     /locus_tag="PKJ16_03255"
     CDS             15839..16624
                     /locus_tag="PKJ16_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46033.1"
                     /translation="MTSASDLGGVGPIVLAIDDDATTLGLITTQMAGLPFRVFTERSP
                     LKAIGTAATLNPDVVLLDVNMPELNGFGVLKELRRDEITRSVPVIMLTSVTQRSVVQD
                     AMRQGVIDYIVKPWDRGRLVGKIESALHYAAMKRNEYRLEESDSIYISRDSDTVILSF
                     RASPKTKEFLAEARSVFSAFFFKSIRGKRCIIDLRPLTEFDAADVNALGVIVRLFGDI
                     RVSIVAGRHYGVIVAASDFEERADLFLSFGDAEMGPAAGGMAE"
     gene            16621..17670
                     /locus_tag="PKJ16_03260"
     CDS             16621..17670
                     /locus_tag="PKJ16_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46034.1"
                     /translation="MSARMLQAVSVDDERTNLMVVEAMARDLGIAVRSFRDPRAALDF
                     VAGNDIDLAFVDYLMPEMDGVELITRIRAHHADVPVVMITSVEDDNRLKVRAIEAGAT
                     EFLTKPLGSAEFRARVRNLVELRTAQLVLRDRAALLEREVRAATAAIRDREYESLIVL
                     ARVAEYKDRETAAHIIRVGEYARIIAASLGEPDEVQDVIFNAAPLHDIGKIGIPDAIL
                     QKDSGLSAEEVAVMRSHPRIGHGILKDSHSPYLQAGALVSLTHHEKFDGSGYPDGLAG
                     EAIHLYGRIVAVSDVFDALTARRHYKEPWTIDDSLAYLARESGAHFDPRLVEHFVANL
                     DAVLAVRERYAEPAA"
     gene            complement(17727..18215)
                     /locus_tag="PKJ16_03265"
     CDS             complement(17727..18215)
                     /locus_tag="PKJ16_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46035.1"
                     /translation="MSLVELPFGLPGAIYRSAMPLGSFDPGGGLIAAYRAAGIATIVM
                     LLPDEECLRRDNVDLRARYAREGFAVLYFPIVNYSVPYSIEATRELIGEIVGRAGRGE
                     RISVHCYGGWGRTGIVLALLARAVCGYSGDQAIEWVRRSVPGAVETDEQESFVREFTF
                     TE"
     gene            18384..19865
                     /locus_tag="PKJ16_03270"
     CDS             18384..19865
                     /locus_tag="PKJ16_03270"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HNV46036.1"
                     /translation="MMSNVNGALNEQQLRAAGGEGGVMLVVAGAGTGKTRTLVEKVGA
                     IVRQGTAAPENILVLTFSRKAAEELRERVRAALGASADAIPMGTFHSFCLGALREAAD
                     DYLGWSGRDRFPAVMDDAARAGLLGELVAGRRERFLGIPDDVVLSLMEGTRRVDPRIA
                     ARLDALGVSAELERLRAEFDERKLAAGLLDFEDMIGHFSRLLRERPAVRRRMIDRYRY
                     ILVDEFQDTAPGNFEVIELLCPDVGRNLFAVGDDAQSIYGFRGARPGYLVEMRRHFPE
                     AAVVTLERNYRSRAEIVRRSDRFIARNRGGVRKHLTAVRGAGGAVVPVPAGSIREECA
                     RLSQIVERERAAGATIAVLYRNNWQGEAVRRECRVADAAGVSLMTMHASKGLEFDAVI
                     VIGVADGIIPDPAADIEEERRLLYVALTRARDRLYVIHRANADGSPARFARELGWGKR
                     RGPVAWLRDAIASARAARGRRREAGSDRTVRSEIRGDAQCESW"
     gene            19850..20113
                     /locus_tag="PKJ16_03275"
     CDS             19850..20113
                     /locus_tag="PKJ16_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNV46037.1"
                     /translation="MRIVVKGKAAKQLCKIEKSNRALAIRIRNKIRRYAADPQGRHDV
                     KPLRSGSVSTRRLRVGVYRVTLVAKEEVLYVLESRHRQGAYRG"
     gene            complement(20122..>21755)
                     /locus_tag="PKJ16_03280"
     CDS             complement(20122..>21755)
                     /locus_tag="PKJ16_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV46038.1"
                     /translation="LYTPDGVLTDVNTAWETIWGLDPQEQIGRFNIRNHEPVISRGLQ
                     NYIDEAFRGESVIIPEMTVERPPDRPGGAPRPMILRCRAFPILEKGGAIQSIVFLHED
                     ITERKQAEEQALYYSMFDNLTGLPNREVFATRLHTEIMKSKRRGNEYIFGVMCLGTDR
                     FKNINEMHGPVTGDKLLKEIAKRLKTTFRADDLVARLEGDKFIVLLSDIKTADDISSI
                     VRKTEDAFRAPLTAAGIELTVSMTIGVCLYPNDGDKAEALIKNSEAALYMAKQRGTGT
                     YHLYDHTMHEQTIHNLRLEKDLVVAIDNQEFRAFYQPKVNREGAIVGMESLIRWQSPA
                     RGLVPPLHFIPLAEKNGLIKDIGYIIMYESCRQTKEWQTMGLPPLKVAVNLSSFQFRQ
                     PCLIDKIKEILSVTGLDPQWLEFEITESGIMENEKESIGKLNEINALGVSISIDDFGT
                     GYSSLSKLKSLPIDTIKIDKSFVDDLPHDATSVNIATTIIDLAHNLGFKVVAEGVETK
                     EQIDFLLYLGCDQFQGYYYSKPLPSDEFRKKLSTA"
     assembly_gap    21756..21855
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21856..22222)
                     /locus_tag="PKJ16_03285"
     CDS             complement(<21856..22222)
                     /locus_tag="PKJ16_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46039.1"
                     /translation="MEQIPGILIVEDEPITALDIKGILEQSGYRVAGIAETGERALEI
                     VRTEPPDLILMDIILGGTMNGIELATAIKERHRIPIIYITSLSDASTFARTLSTDPYG
                     YITKPLDTTMLTHMIDLALY"
     gene            22362..22541
                     /locus_tag="PKJ16_03290"
     CDS             22362..22541
                     /locus_tag="PKJ16_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46040.1"
                     /translation="MDRFISDLIREYHVLFKNIFGIGGLILAWICITGFIRFCIAAWR
                     RRKGVYPPAASMEDH"
     gene            complement(22591..>27249)
                     /locus_tag="PKJ16_03295"
     CDS             complement(22591..>27249)
                     /locus_tag="PKJ16_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46041.1"
                     /translation="RSDEVAGTFGYMAPEQCGVIKRAVDERSDLYSLGILFYHLVTRR
                     MPFTGDTVSALIHQHIARLPEPPRAIDDTIPPVLEQVICKLIAKEPERRYQSARGLLA
                     DLDRFLGGRDDFVLGTNDRISRLNYRTVLVGREKEYDALVARYTGVMRGTGSVCLVGG
                     EAGRGKSRLIEELCTYVRGMNGLYIDGKCFSGANKPPFGPLKEAANVYLRFFGKYSAL
                     RQESIRTRLRAALDPLSEIVTQFNPPCAALIGAGETPVPLDPEKEAARFLSVVARFFR
                     TLGEIERGLVIVLDDLQWCDEGTIAVLLEIIQGIDQAPLLVVGTYRDNEIAPEGPLAR
                     FIRQAGERSLPVHDIRLELFDRARMRQFVEGLLFDTGPAIGGIADLVLSKSGGNPFFA
                     IEILKQLVDEKALVPGAHGWSVDRAVLEDAQIAPTILDILLKRIATLPPDDIALLSYA
                     SVIGRKFEIEILFRLMPAYSIPAIVSIVDRAVRLQLLEEDRNDRGKIIFVHDRIREAF
                     YQNIAAAELSALHGKIAATLEELHPEPGDDALFEIAHHWIAAGDREHSLRFAHPAGLR
                     AMRRYANDEALKYFTTVTALLSESGDTDSPLMLECLRHTADILLMTGRHDEAIALYTT
                     LRARMGAKYEQAAVIRQISDAYWKKGDFPSCERTAAEGLSLLGERLPLGKIAVAAGTA
                     VELCTHALHVLAPRLFVRRRDSRSERHKLMIAYYHSLNWSYIYSDLLKFIRSILRMLN
                     ISQRRIGPSRELGLSLGGYASLCMAIPRFGRALTYHRRSLAMRDELRDEPGLVESHQF
                     IGYCYQWMGEYRKSIEHFEESLSRARTIGDMLLLNQSVNGLLQSYYYLSDYENGMTYL
                     DEFFAVLERRKDLFSVLWGALYRTQYAVERGDYAGAESTATAALPLCTAHGMWIQYCI
                     LNTELAGVYLEQGDTARAREHIETARRLFEKNNFMRHYTVHLYTRYAEVLIAEHLARA
                     GTGRGSRGALRAIRRACRTALRKTKSWPAHHAGALRESARAQALSGNHRAAGRLFQRS
                     IDHAERIGRRHELARAMYDYGRFLADTGHAESARKRLETAYRIFSEIGAAGFLKKTEE
                     RLGMGGGEAPSAAQRLMDRQRLSSIITVSQDISSILDIDELIDTILAKAIEVTGAQNG
                     ALFLVDETGAELVVRAATGPDADEAAHSRHIVADVFRHAKTVISLNAEADRALGEYHS
                     VVEQGLKSVLCVPIRQHERVLGVCYLDNRLSSGVFTDEDARVLEALMAQAAIAIENSA
                     LYNNLERKVDERTSELTTAYQKLNEAYQKVEEQSLELKDAYDKLDLAYHSIKKDLTLA
                     RHIQQNILPAAADRIGDLGLAIQYIPMGEIGGDLYDIAVRDGGALRVFIADATGHGVQ
                     AALVTMIIKSEYEMLKNAIASPAELLETLNSEFMAKYASLVVFFTGVVADIDTARGSI
                     TYASAGHPDQFLIRGGAVKPLQRTGRAVGLHDSINISTRTVAFGPGDRLILFTDGLFE
                     EFNATKELFGEERLGRLALTNAGEPAERIIERMLAAMRDFIGDEELNDDITIIGVERE
                     "
BASE COUNT         4397 a         8673 c         9430 g         4649 t
ORIGIN      
        1 tctcccacgg gcgccggtgg gggaagcgcc ccatcgtcac gaattcctcc accgtgaagg
       61 gcaacacgtt ctcgatgtgc tgatggacca ccgcgagctc gcgcgcgagc tcgcgctcgg
      121 tgtaggcggc gagggcgcgc ccgcgaaacg cgatcgttcc cgccgacggt cgcaggttcc
      181 ccgtgaggag cttgagcagc gtgctcttgc ccgcgccatt ggggccgatg acgccgagga
      241 aatcgtccgc atgaatgcgc acggaaatcc cctcgagcac gttcgtcccg cgataccccg
      301 cctcgaccgc gccgatctcg taggcgatca tcgcgcatcg ccccgcgtcg cgaacaggac
      361 caggaaaaac atcccgccga aaaacccggt catcactccg gccgggacct cggagagcgg
      421 caggatcgag cgtcccagcg cgtcgcacaa cgcgagcagg cacccgccgc cgagcgcgct
      481 ggcggggatg agcaaccggt gcgcggggcc gagcgatcga cgcatgatgt gcgggacgat
      541 gaggcccacg aacccgatga cccccgccag cgtgaccgcg agggcggcga ggagggccga
      601 gagccagaag atgttcttca cggcggccgg cgagacgccg agggcgcgcg cgaaggcggg
      661 cccgaacgag aggatgtcga gacgtcgatg gaaaaccatc gcgccggcga tcagcagcgc
      721 gacgagcgcg cccagcggcg ggaggagcgc gtagcgggcg atggagaggt cccccatgag
      781 ccacatgagc gccttgtgca ccagctgcgg ccgggcgacg gaaaagacca gcatgacggc
      841 tgaggacagg atgaagctga gcgcgatccc ggcgacgatg agcgacacgc tggagaagcc
      901 gcggcgcgcg gcgagggcgt tcaccgcgat caccgccgcg aggctccccg cgaaggccgc
      961 cgcggtgacg gcccccgcgc cgagggagag gcagatggcc acggtcgcgc cgagcgccgc
     1021 gccgcccgcg gtcccgatgg tgtaggggtc ggcgagcggg ttgcggaaga gcgcctgata
     1081 gagcaggccg ctcaccgata gcgcggcgcc caccgcgaac gccatcagca tgcgcgggac
     1141 gcgcagcgtg gcgatgatga gccgctcggt cgaatcgccc ccgccgatga gcgcggccag
     1201 cgccgtgtgc ggcgggatga aggtcgcgcc cagcgagatg cacagcgcga acaccgccgc
     1261 gagggccgcg atgatgccga tgacggcgag gctacgtttc atcgatgcgg cgctccaggc
     1321 tcgtcaggat cgacgcgagc gcgctcgccg cgtcggcgta gccgtccggg gtgtactgcg
     1381 ttatgtgcga gtggtccagg gtgaccatcg cgccccgtcg gaccgccgga attgccgtga
     1441 gggcgccgtg cgcgtccggg aacagcggtt ccgggttgtc ggtgaaggag atgagcacgt
     1501 cggggcgggc cgcgacgagg tgctccaggg tgatgaccgg gtaggggatg gaactggcgc
     1561 gaaagacatt ttccccgccc gccgattcga tgatcccgtt gatgaaggac tggccggacg
     1621 ccgcgacgag cggaacgtgc gacaggagga ccgcgatccg gaggcgccgc gcggggcgct
     1681 tgatcgcggc gatccgcgcg cgcaccgcgg cgatgcgctc ctcggcgatc gcgccgcgcc
     1741 cgaggaggcg ccccagcgat cggaagttgt cgcacagctc ctcgaaggag cggttcgcgc
     1801 cggtcacgaa cacgggcagg cccgtcgcgg ccaggagctc ggtgaactgc gtgtcggcgt
     1861 cctcccgcga atacatgacg acgtcgggcg cgagggcgac gatccgctcg atcgacgggc
     1921 ggctgaggtc gcccaccggg gccacggggc gtccgagggc gggatggtac gtggtgaccc
     1981 cgacgatcgc gtccgcggcc ccaagctcca tgatctgccg cgtgatcgag ggcgagagcg
     2041 acacgatgcg cccgtgcgcg cggggggccg gcgcgggacg cgcgccggta tccgcaatcg
     2101 catagagcgc tccgaaaacg agcagggcgg cgaggagggg caggcgcgcg gcgattgcgt
     2161 gtttcattta tcctcgaagc gtgacggttc cgttctccgc gatgtccacg cgctgtccct
     2221 gccggaccgc gccgaagacg tcgccgaatc gaaccaccga cgcgagcgcc ttctggaggg
     2281 cgcttccgtg cgcgccggcg aggatgtccc gttcgggttt cgtcagtttc attgcggtgc
     2341 cgtcatgtca tcacgcacta tcacgatcgc gaaaatgcgc aataattttt tccgcggcgc
     2401 aatattttgc ttgtccatgc ggtgctctgt ggcacgcttt cgtgtcgata cgtacgatca
     2461 tcgagaggtg gacggtttca tgaaacgcgc cctgcggatt gccctgtcgc ttgtgttggt
     2521 gtcggtcgcc gcgcttgtcg ggatcaacat ctatgtgctg gccgcgaccg cgccgttcat
     2581 ctttgacgag gcgaaggacg tgccgcgcgc ctatgccgtg ctcgtgccgg gcgccttcgc
     2641 ctatcgcgac ggcaggctgt cgcatgtcct gtacgaccgc gtccagcgcg cgcaggacct
     2701 gtaccgcgcc gggacggtga agcgcttcct gctctccggc gaccacggcc ggaagacgta
     2761 caacgaggtg aacatcatga agagccacct catgaagcgc ggcgtgccgg gcgcggacat
     2821 cttcaccgac catgcgggct tcatcaccta cgactcgatc gtccgcgcga agcgggtgtt
     2881 ccaggtccgc gacatggtcg tcgtcacgca gcggtatcat cttccccgcg ccgtttacat
     2941 cgcgcgcaca aacggcatcg atgcctatgg cctggcggcc gaccggcgca cctatcgcgc
     3001 gatcctgtgg taccgcgtcc gggagttcgt cgccgtgggt aaggcgttcg tgtggctgct
     3061 gatcgacaag cggccgcgct ttctcggtac gcccattccc atcaccggcg acagcgcgaa
     3121 gagctgggac tgacgcggcc gctcccccgg ctgacggcca taccccgatg gcgcgcgacg
     3181 caacttcgat ggaacgacta cgctactacg cagacatgct ccgcggcccg gccttcaccg
     3241 cgcacctcgc gcgcagctgg gcagtgggct ggcccatggt gttcatcatg gcgctggagt
     3301 tcctcatcaa cctcacggac gtcttcatcg cgggccggct cgggaaggag gtgcaggcgg
     3361 cggtcgggtt cgccatgcag ctctatttca tcttcatcgt cgccgccaac gccatcaccg
     3421 tggggacggt gtcggtcgtg tcgaggatcc acgccgccgg cgacggggag ggcgtgcgcg
     3481 cggccgcctg gagcgtgatc gcgacatgcc tcggcgcggg cgcggcgctc ggcgttctcg
     3541 gatacgccct ctcgccggtc gtcatcggca tggtcaatct cccggccgag gtgaagcgct
     3601 acgcggcgcc gctggtccag gtcctcgcgc tcgggctccc gttccactac ttcaacatca
     3661 acgcgagcgg gatcatgcgc gcgacagacc gcgtgaaacg tgcgctcgcg acggttatgg
     3721 cgacctgcgt catcaatatc ggcctcaatt ttttgctcgt atttcatacg ccgctcggct
     3781 ttctcgggat catggtctcc accgtcggcg cgaccgtggt cggttcgctc atcaacgcgg
     3841 gtttcatggc gcagatcctg cgcgcgccgc gcgtattctc tcgcgggctg ctcggcagga
     3901 tcctgctggt gggctggccg tcgggcgtgc agcaggtgat gtggcatgtc ggcgcggcgg
     3961 cgctctttct catactgggc gcgctgccga accacaatgt cgaggtgatc gccgcgtaca
     4021 cgaacgggct gcgcatcgag gcggccatct tcctgtccgc cttcgccttc aatatggcca
     4081 atgccgtgat cgtgggaaac ttcctcggcg cgcaccggca acgggacgcc tttcgcggag
     4141 ggctcgtcac ggcggggatc ggggtcgcgg tggtggcggc gctcacgatc gtggtggtcg
     4201 tgcacgcgaa accgtttgcg ggatttttgt cggccaatgc aatggtgata gaccggtgcg
     4261 ccacctacct gtacatcagc atgctcagcg agccctttat ggcctggggc gtgatcctcg
     4321 ccggcgcgct cggcggcgcg ggcgacacgc ggggggtgat gatgatcatc gtggggagcc
     4381 agtggctggt gcgcctgccg ctcgcgttcg tgttggggat cgtgctggga ctcggcgagg
     4441 cggccgtctg gtggtccatg aacgcgtcga ttttcgtgta ttgcatattg cttacgcgcc
     4501 gatatttcaa gaggaaatgg ctccatctat gaaacgcgaa ccgctcgacc tttcgcaccg
     4561 ggaactcctc gccggccgcc tgcgcgccgt cgacaccgcg ctctccgagt acagcttcgc
     4621 gaacctgtac ctcttccgcg acaatcaccg ctacgaggtg ctcttcgatg gggacgacgt
     4681 gttcgtcgcc gggctcacct acgacggggc gcgcttcatc atgcccgccc gcaacgtcga
     4741 tcccgcctgc gtcgagcgcc tcgccgcgct gctcgcggat ttcgacatca tctttcccgt
     4801 gccggagccg tggctgccgc tcttcccggc cccgcgtttc gcgcacgcct acagcgacgg
     4861 cgacaccgat tacgtctaca ccatcgaaaa gatgagcacc tatccgggca agaagctcca
     4921 caagaagcgc aatctcatga agcagtacga ggagctctac gagcacgagg agttcccgct
     4981 caccgacgac cggatcgccg acgcgcgcgc ggtgctggag gagtggcaaa ccgacatggg
     5041 cgttccgccg cgcgagaccg actatcgctc gtgcgtggag gcgctggagc gctacgagga
     5101 tctcgcgctc tgcggcggga tctactacgc ggcggggcag ccggcgggct tcatcctcgg
     5161 cgaggagctc gacgcgaaga ccttcgcgct ccatttcgcc aaggggctcc ggcggttcaa
     5221 gggcgtctat cagttcatgt acaacagctt cgcgcggatc atgcccgcgc agtactgctg
     5281 cctcaacttc gaacaggacc tcggccggga ggcgctgcgc caggcgaaga cctcctacgt
     5341 gccggaccag ctcttgcgaa aataccgcgt gcggctgcgc tagccgcacg cgcgtgcgcg
     5401 gctagcgccc gacgggctac ggttttctcc gattgcgatt gtagtccttg atgaggcggt
     5461 ggagctcctt ctcctttcgc tttttctcgc ggcgcgcatt ctcgtcgatc tgcgactcga
     5521 gataggcgag ctcccgcgta agccgatgga aattctccag ccgacccgga tcaagcgttc
     5581 cgctctcgac ggcctgccgg actgcgcatc ccggttccgt ttcgtgcgcg caatcgtcga
     5641 accggcactg cgcggcgagc tcctcgatgt cgcgaaagga gtcgtccagc cccgaggcgt
     5701 cccacggcat gagttcgcgc atgcccggcg tgtctatcac catgcccccg ccggaaagaa
     5761 agaacaactc tcgcacggtg gtggtatggc ggcccttgcg gtccccctcg cggatttcgc
     5821 cgatgcgctg ccgctgcgtc cccagcatgc cgttgatgat gctcgacttg cccgcgcccg
     5881 acggtcccag caggcaggcg gtgacgccct gcgcgacatg cgcgcgaatg tccccgatgc
     5941 ccaggcccgt gagcgcgctg gccgcaaccg ccgtgacgcc ggggatccgc tcggcgatcg
     6001 cgcccagcgc ctccgcggca tgcgcctccg tgtcgcactt gttgaggacg agcaccggcg
     6061 ttatgtccga acccttcgcc aggacgaggt agcgttcggc gagggcgcag ttgagcgcga
     6121 gcgcgtcctg gacgacgaac atgatgtcga tgttcgcggc catgacctgc cgcgccgatg
     6181 ttttccccgg cgccttccgc gaaagatggt ttctgcgcgg gagcacgtcc tgtatcacga
     6241 gccaggaatc gtcgcccgtt ccgcgggcga cgacccagtc gcccacgacg ggcatctcga
     6301 tgtcgtggta gtggaatttt cccgcgggaa cgccgaagcg ctcgccctgc acgcccgcga
     6361 gcagccaggt cccccgggag acggagatca cccgcgccgg gtgcgagtct tcagttttat
     6421 gttcggaata gaaccgctcg aaatccgggc cccagcccag gcgagcgaga ttgtcatgtg
     6481 acattgtggt agcctctcat caaaaaaatg cccgaccatg ccgatccgcg gccagcgcgg
     6541 tggcggttcg gggtgcgcga tgatgaaatg gctacacaat catatgcgat ttggttcgtc
     6601 gtgcggtatg atgccggcgt gcgaacgccg gtgtcaatag aaatttttcc gattcggcgc
     6661 ggcgtgcaat tgctcatgcg atgatccgcc cacggcggcc gcatcgagcg ggactcgcgt
     6721 cgattaaaaa acccgcgtgc ggacgcgcgc cgcacgcggg cgatcgcggc ttactccccg
     6781 ccgcccagca cgaatccctt cgatgccggt ccggtggacg ccgaggtgcc ggcgatgcgc
     6841 gccaggtagg ccgcgcccag cttcccgagg tgctcgttca cgttatcgaa gtagttcagg
     6901 tgcttctgct cttcgtcgat gatcgtttcg aaaagctttg cggtgatgct gtcgccgttt
     6961 tccaggcaga ccgccaggaa ccggttgtag gcgtcgatgg cgtcgtcctc cgccttcgcg
     7021 tcgaagggga agatcgcgtc cacgctctgc gccttctcga cgccgccggc gaggtcgctg
     7081 gtgggctcgc cgccgagctc cttgatgcgc tcggcgaaca tctcggcgtg gcgcatctcg
     7141 tcgatggcga tgagcttgat gttgcgcgcg agctcgccgt agtccaggtt gtccaggtta
     7201 taatgctggt tcatgtactg atggatggcc tgcagctcca tcgagcgtgc ggtgttgagt
     7261 acggtgacga ctttttgctt gcgttcttcg cgtgatgcca tggttctctc cggtgcgtcg
     7321 tatggtatga tgcgattggc ggaatgccgc gatggggctt catatagcac acgcgcgggg
     7381 cgctgtcaaa gaatttattt gttgcgggtt ccgcgcgatg cgatacggtc tgcattaatg
     7441 aagtgcagat gataccagga ggcgccatgg acaccgccat cacaacgccg gagcggctgc
     7501 aggagctcat caacgcgtac cgcacgtcgc gcatcatact cacggccttc gagctcgggg
     7561 tgttcaccgc cgtggggacg ggtgatgcgg acgctgcgcg ggtcgcgcgc gccgtgggcg
     7621 ccgacgagcg gggaacggac cggctcatga acgccctctg cgcgatcggg ctcctcgaaa
     7681 agcgcggcgg ccggttcttc aatacgccct tcggggccga gcgcctggtc aggggggcgc
     7741 cgcgctacct cgaggggatc cgccactcgg tgaacctctg ggacacctgg catacgctca
     7801 ccgacgccgt gcgcgcaggc catacggtgg tgaacggcga tccggcctgc cgcgacccga
     7861 actggttcga gggcttcatc gccgccatgc atcagcgcgc ctcggcgcag gcgccgcgca
     7921 tgatcgaatg catggacctc gacggcgtta tgcgcgctat cgacatcggc ggcggatcgg
     7981 gggcctatgc catcgcgctg gcccgcgcgc gggaggggct gcgctgcacg gtcttcgacc
     8041 ttcccaacgt gatcgagctc gcgcgcggct atgtcggccg cgcgggcatg ctcgagcgca
     8101 tcgatttcgt cgccggcgat ttcaacgcgg acgatttcgg cgtcgggtac gacatggcgc
     8161 tcctctcggc catcatccac atgaattcac ccgacgggaa ccgccggctc ttcgcgaaga
     8221 tcgccgcctg cctcaatccc ggcggccggc tggtggtgca ggatttcgtg atgaacgacg
     8281 accgcaccgg gcccgccgtg ggcgcgctct tcgcgctcaa catgctcgtg gggacctccg
     8341 ccggcgacac ctataccgcc gccgagatcg agtcgtggat gcgcgaggcg ggatgtgccc
     8401 ggaccgaggc gcgcgacact ccgttcgacg cctcgcttgt catcggctgg aaatagaatt
     8461 ccccccgtcc ctcggacgcc tcccccttcg atgggggagg gaggggcatg gcgtgccggg
     8521 tgttggtccc ggcgcgttag gacatgaagg ggtttccgaa ccggtcgagg tgcgtgaact
     8581 cggcgaccgc ccggcgcgcc ggtccctcct tcgcctcgac ggccggcttg ccgataggga
     8641 tcgacacgac gacctcgtag tcctcgggga ggtcgaggag gtttttgcag atgtcgtggt
     8701 tcatgagccc cacgatgacg gtgcccagcc ccagctcgtg ggcgcgcagg cagatggttt
     8761 gcaccgcgag tcccaggtcg aacatgtacc agctcgaaaa cttcgtggcc cagccgccct
     8821 ccttgacgcc cgccttcttc gaatgcgcac agccgatgat gagcgccgag gccgcgagcg
     8881 agcatttccg cgccgggttg aattccgagt acgccgcggt gatcttctcg atgaggccgc
     8941 ggtcgcgaac cacgatgaac tcgcagcact gcgtgttcgc ccacgagggc gcccagcgcg
     9001 ccgcttcgag gagttccgcg atctcggcat cggtcacgac gtaatcggtg aattttcgaa
     9061 tgctgcgccg cttcagtatc gcttgcgtga gttccatggt tcctccgtgg tgttgatttc
     9121 acctcacccg cactcccctc gacttagctc ggggatcgcc gggccgccaa ctcccacagg
     9181 agagggcttc aaatcagggt gtcccttgcc gctccccccc cttctcctat gggagaaggg
     9241 ggtcgggggg atgaggttta tgaattctgt cgaggattgt atatctgtca actgtataat
     9301 gcggcgttcc gccgccgctt tcgaaagcgt tcaaggatga tgatgagcgc gagcgcacac
     9361 gcaacgacga gcgccgggtg cggttcgtcc gcgcgctcgg tgatttcgag cgtttcccgg
     9421 ttccgcaatt tctgcttctc cttctcctcg aggatgcgcc actgcgacgg gctctccacc
     9481 acgatgtacg atgccgcgcc gtttaagatg ccggtccgct tggctgtcgc gacgagcgcc
     9541 ggatagagcg cccgcgtccg ggcggggatg agcttcaggg tccggtaatc gacgatcccg
     9601 gccaggccga cagcggcttc actcggcccg gtccgcaggg gccgataggc gcgcgtcgat
     9661 gcgtcgagat acgcgatcga gtccatcgcc gcggcgccgt cgcagcggat gagttcggac
     9721 gcgccgtcgg tcgatatcgg cacggtgcgc gctccgcatc tgacgagatg cacggggcgc
     9781 ggaagcgggg agacggcgcg cgcgcgtttt ctatcgaagg gaacgggagc gagcgccgag
     9841 ccgttttccg tcataaagta gccgtcgatg tcgggcgcga gcccaccgaa gaatgagaga
     9901 ttgtcctcct cctcgcgcgt gggggtccta gtcgcgtcgg gtgcggcctc cgtgcgaccg
     9961 atgacgacga tgatcggcgc cgattccggg tcgcggtgcg acgtcaggat gcgcttcacc
    10021 tcgcgttgcg cggagaatcc gccgcgccgc gtgagcgccg aatcgatggc gcccgccgta
    10081 atcgacgcga tacgatcgcg gctcgatgtc ggcgggagga tgtccacgct ctcgaagttc
    10141 gcggcgctga gcgcgcagcg cgcaatccgc gggaaccgct cgaggacggc gcgcgcggcc
    10201 gcgagcgcgc gtgcggcgtc gagcccggtc gcgtccgagc ggtccagcac gaggtgcagg
    10261 tagggcgcgc gcgtcacggg gagcatcgcc cgcttcgccg ccggcggaag gaggacgccc
    10321 gtcgcgcccg tcgcggggac gatgtttgcg gtatcggata acgccgcacc caggtcgatc
    10381 cgccgcccgc cgatcgtcgc cgcgccgccg cctcccggcg ggaccgtaag ctcgatctcg
    10441 gtcacgcgct cctcgtgcgg cccgaagggg aacacccgga gggcgagctc ctgttccccg
    10501 gtgaagtaca ggatgccggg atcgcgccgg acggacttga tcatgcggta cacccagagg
    10561 gcggtcttct tctcgaagat gcggccggga acgcgctcct tcccgatgtg caaccagtag
    10621 cccgtcacaa gcgtcccgac gggcagcgtg accgtcgtcg cgtattcacc ctggagcttc
    10681 gtccggtttt tcatggtgat gacgacggtg cgcttcaccg cgtcccccgc cggcgcatgc
    10741 cgcatcgcga ccgaaacgag ctcaaccctg ccgggaagcg gcagcgggac gcggccgccc
    10801 tcgcgggcca ccgaggtgcg cctgccgaag atatcgccca ggacatttcc cttaccgtac
    10861 ggggccatcg gcccgccgaa gaagaagcgg tgcaggcggt ccatcttctc gtccggaagc
    10921 accaagttgt cgaagacgat ccagttgtag tagccggtga gaaacggcat ctggacgccc
    10981 ttcttcgttt cctgcaggtt cgcgaggctg tgttccagga ggaagcggct gccggagaaa
    11041 acgcggtcct tctcgtagtc gggttcgtag acataatcga gcgcggcgtg gatattgacg
    11101 cggtcgacca gggcgcgcgc cgtgtacgcg atcggcagga cgagaagcgc ccccgcgagc
    11161 atgatccgcg tcgcgcgcgg cgcgccgcgc gcgatatcgg cgcggtagtc gtcgacaagc
    11221 ctgcttccgt gcaggagaaa gaggaccgtg ggcgtgagga tgaggaaacc cgccccggcc
    11281 gccagtatcg cggggatgcc gagcgggaga aacggcagga acaccgcaaa gaaatagagc
    11341 gtgaagggaa acatcgccgc gcgcgtcagg aagatggcgc gattgaggcg cacggaacgg
    11401 gcgggcggca gcatcaggac aatgccgttc gcgacggcga gcgcatacac cgcggggctc
    11461 tggaagtccg tcgggaacgg gatggcgcga ttgaggagca ggcccccgat ggggccggcg
    11521 atgccgaagg ccgcatgcgc cgcgaggctg agccagcggt tctcgcgata gaagcgcgca
    11581 taaaacagcg cgaacaggcg gatgatcgcg aggaacatga gcgccgtcgt tgcgatgaag
    11641 aacgggagga ggacggcggc gacggcggcg ccgctccaat tcctgctgag gctcgattgc
    11701 atcagcagga cgaagaggta ccagaacacg ggcgggcccg ccagcgagac ggaccagacg
    11761 gcgacctcct ggggaaagcg aagcgttgtg cgaaacagag cggccgtggc cagcgcgaga
    11821 aaggccgcgg gcatgacggc gatgaactgc gcgtagacga cgccgtcgta ggaaaggatc
    11881 cattcgggca cgttgcgcgg aaacgccgcc gggagcgtct ccgtgaagag gtacaggtag
    11941 gccgccgcgg cgaaaagcgc cgggaggccc gccgcccagg ggatttcgcg tcgtcgcgcg
    12001 ccgaacaggg cgccaaggac ggcgaccaac gccagaaggg cgatctcggc gatcaacagg
    12061 gagagcgcga ttcggttttg gtgcggatcg agctccccgg ctaccagcga gtacgatcgc
    12121 aggttggcga ccagcaggag cgcctgcggg acggtcccga agatgatcca gttgatgggc
    12181 cgtctgacaa cggcgatgag ggcctcgatg cgcgcggcgg tgttagcgat catggacgaa
    12241 cctccgttcg aacaggtagt aggccaggac gagcgccggg acgaagacga gcacgccgat
    12301 ccacaggtgc gccgtgttcg cgatcgcgcg cggcgtcccg gggggcatga tgatgaatgc
    12361 gtagaggcac gcgacgagcc ggagcgcatt cgcgagcagt gcggcgggca acgcggccag
    12421 gacgatcccg atcgcggccg ggacggcccg gcgcgcatca aaccgccgaa acgcctggaa
    12481 taggagaaat gagcagagga tcgtataaaa ggtgaacccg ctgcactcgg gaacgacggc
    12541 gaggcgcagt gcggggtggg ggatgatgta tcccgcgccc ggatcctcga cgcagggcag
    12601 gcccaggtag agcgcggcga tgcgcgcggc gggatgtaaa aacaccgcat gaacgagttc
    12661 cgccggcgcg agcgatatcg ccccgaggcc gattgatgcc gccatgacgg ccgcgatggc
    12721 ggtcctcccg cgcatgtcgc gggaatgccg caggccgggc gcggccggga tggtgtccgt
    12781 gggctggttc acgcctgcaa cggtatcctc tcgttcgcgc gcgatgcaag ccttattttt
    12841 tgtttgcctc gcgcgggccg gtgcggtatg gtatggattg gcagcggagg aatgatgccc
    12901 tggaacccgt ctcaggcgct cccgttcgat agcatcggag agatcgtcgc cgacccgctc
    12961 gtctcctgcg acgagaccgg cgtgatcgag gcgtgcaacg ccgcctttgc gctgttcgtc
    13021 ggggcggacc gggcgtcggt gccggggcgg ggccttgtcg atttccttcc ggacaatctt
    13081 gtggacgcga ttgtcgcatc gtatgcgccg gatgcggggg agatggaggt gacgctcgcc
    13141 ggccacgcgc cgcgcgccgt ttcctatcgc cgggcattca tttcggacgg cgacggaaac
    13201 cggacgatcc tgtgccgctt caccgacatc accaagcaga agtgggagcg cgagcagatc
    13261 cgcatgttca actacgccgt ggagaacagc ccggcctcca tcgtgatcac cgaccgcaac
    13321 ggcaccatcg agtacgtgaa ccggaagttc accgaggtga ccggttacgc catgaacgag
    13381 gcggtggggc aaaacccgcg catcctgaag tcgggccgcc agcacgcgga ctactaccgc
    13441 gagatgtggg agaccatcct cgccggccgg tcgtggtacg gcgagttctg caatgtgcga
    13501 aaggacggga gcctgttctg ggagatcgct tcgattggcc cggtgatcgg gatcgcgggc
    13561 gagatatcgc acttcatcgc cgtgaaggag aacatcacct acctcaagga gatcgaggag
    13621 gggttgcgtg cgtctgattc gcggacgagc gccatactga acgccgtgcc ggacctcatg
    13681 ttccgcgtgg atacggatgg tcggctcctc gacacggagg tggccgcgaa ggtgcaggag
    13741 atcttccccg atgcgatcgc cgagacggcg cggcccgtga tcgcgcgcgc gctcgaggat
    13801 ttcgacgtga agatcttcga gtaccccgtg tacttcaata aaaagctgca gtactacgag
    13861 gcgcgcttca tcgccgccgg cgacgacgag gtgatcgcca tcatccggaa catcagcgac
    13921 cgggtggcgg cggaggagca gttgaaaaag gcgcgcgacg acgccgaggc cgcaaaccgc
    13981 gccaagggcg agtttttggc caacatgagc cacgagatcc gcacgccctt aaacagcatc
    14041 ctgggcttca tcgagctgct caagctcgag aacatcaccg gcacgcaggc cgagtacctc
    14101 ggcatcgtga gcgagagcgc ggcgagcctg ctgggcatca tcaacgacat cctggacttc
    14161 tccaagatcg agagcgggcg cttcgagatc gaggcggtcc ccttcaaccc gcgccgcgag
    14221 ttcgagtcgg tgatcgacct gttcacggtg aaggcgcagg agaagcgcat cgagttcctt
    14281 tccttcgtcg atccggcgct gccgccgtcg gtcgcgggcg acccgctgcg catcaagcag
    14341 gtgctcgcga acctcctgtc caacgccgtg aagttcacgc cggccgggaa gaccatctcg
    14401 gtcgaggtgc tccgtgtggg cgatgccgat ggcgccgcgc gcatccgctt cacggtcgcg
    14461 gacaccggga tcggcattcc ggagcgcaag cgcgacatca tcttcagcgc cttctcgcag
    14521 gtggacagct ccgtgacccg ccgcttcggc ggaacgggcc tggggctgtc gatcagctcc
    14581 aatctcgtcc ggatgatggg cggcgagctc gccctcgaga gcgagcccgg caaagggagc
    14641 tcgttttttt tcgagctcgc gctgccgcac gccgcggccg aggagagcga gtcgctgccg
    14701 gagatacccc cgtcgatttc ggcggtcgtc ctgggtccgg acgggccgcc ggggccgcgc
    14761 gaatcgttgg ttgcgcgata tctcacgtcg cttcggttta cctggcggcg catcaccccg
    14821 acggacccgg agcacgaacg cgaaccggac gtgttcgtcg tgttcgggga gcaggaactc
    14881 gacggattcg tcgcgcgggt ccgcggcgag cgcccggagg tcccggccgt cgcggtggtc
    14941 ggctcgatgg gcgagatcga cacgctgccg cgcctgcatg cgcgcgaccg ggtggtgagc
    15001 ctgccgctgg gcgcctcgaa gctgtacaat gcgctggcag acgccgtggg gatcggcgcc
    15061 ggtgcggggg cgatcggacg cgcggcctcc gcgcgcgcgc tgcccgcgct gcgcgtccat
    15121 gcgctcgtcg ccgaggacaa tgccgtgaac cagaagctca tgctgctcat gctcaagcag
    15181 ttcggcgcga ccgccgatat cgcgtccaac ggcctcgacg ccttcgaccg ctaccggaag
    15241 gggcgctacg acgtcgtgtt catggacatg cacatgccgg tgacggacgg cgtcgaggcg
    15301 gcgcggatga ttttggacta cgaaaaggtc tatcggctcc aacacgttcc catcgtggcc
    15361 ctcacggcga agatgatgag gggcggcgcg gacgagctcc tcgcaatggg aatggacgaa
    15421 tacctgccca agccggtgtc cctcgcggcg ctcgaggcgg tgttggtgaa attctgcgcc
    15481 tcccgggacg gcgccgaggc cgccgaggcc gctcccgtcg cggacgccgc gggcgcgtat
    15541 tccttcgacg attccgccgc cgaactcggt attggggttg acgcgctccg ggagatcgtg
    15601 cgagagttca tggccgacgc ggacgagacg atggcggcga tcgagcgggg cattgcggac
    15661 gggagccatg aggccgtcta tcaggcggcc caccgcctca agggggccgc cgccaacctg
    15721 cgctttcgcc gtctggccga tgcgctgcgc gcgctcgagg cggcggcgcg cgcgggcgac
    15781 gcgggtcgct atcgcgccct cgtcgacggc gtcatcaatg aattcgggag cgtgcgcaat
    15841 gacatcagca agtgacctgg gcggcgtggg gcccatcgtg ctcgcgatcg acgacgatgc
    15901 gacgacgctg ggcctcatca ccacgcagat ggccggattg ccgtttcgcg tgttcacgga
    15961 gcgctccccc ctcaaggcga tcgggacggc ggcgacgctc aacccggacg tcgtcctcct
    16021 cgacgtcaac atgcccgagc tcaacggctt cggggtgctc aaggagctgc ggcgcgacga
    16081 gatcacccgg agcgtcccgg tgatcatgct cacctccgtg acgcagcggt ccgtcgtgca
    16141 ggacgccatg cggcagggcg tgatcgacta tatcgtgaag ccgtgggacc gcgggcggct
    16201 cgtggggaag atcgagtcgg cgctgcacta cgccgcgatg aagcgcaacg aataccggct
    16261 cgaggagagc gactccatct acatctcgcg ggacagcgac acggtgatcc tttcgtttcg
    16321 ggccagcccg aagacgaagg agttcctggc cgaggcgcgc tccgtgttca gcgccttctt
    16381 cttcaagtcg atccggggca agcggtgcat catcgacctg cggccgctca cggagttcga
    16441 cgccgccgac gtgaacgcgc tgggcgtcat cgtgcggctc ttcggcgata tccgcgtgag
    16501 catcgtcgcc ggtcggcact acggcgtcat cgtcgccgcc tcggatttcg aggagcgggc
    16561 cgacctcttc ctctcgttcg gcgacgcgga gatggggccc gcggccgggg gcatggcgga
    16621 atgagcgcgc gcatgctgca ggcggtctcg gtggacgacg agcggacgaa cctcatggtg
    16681 gtggaggcga tggcgcgcga cctggggatc gccgtgcgct cctttcggga cccccgtgcg
    16741 gcgctcgact tcgtggccgg aaacgacatc gacctcgcct tcgtcgatta cctcatgccg
    16801 gagatggacg gagtggagct catcacgcgg atacgcgcgc atcacgccga cgtgccggtg
    16861 gtgatgatca cctcggtcga ggacgacaac cgcctcaagg tgcgcgcgat cgaggccggc
    16921 gccaccgagt tcctcaccaa accgctgggg agcgcggagt tccgggcgcg cgtgcgcaat
    16981 ctcgtcgagc tgcgcacggc gcagctggtg ctgcgcgacc gcgcggcgct cctggagcgc
    17041 gaggtgcgcg cggccaccgc cgccatccgc gaccgcgaat acgagagcct catcgtgctg
    17101 gcgcgcgtcg ccgagtacaa ggaccgggag accgcggcgc acatcatccg cgtcggggag
    17161 tacgcgcgga tcatcgccgc ctccctcggc gaaccggacg aggtgcagga cgtcatcttc
    17221 aacgcggcgc cgttgcacga tatcgggaag atcggaatcc ccgacgccat cctccagaag
    17281 gactccggcc tgagcgccga ggaggtcgcc gtgatgcgga gccacccgcg catcgggcac
    17341 gggatcctca aggactccca tagcccctac ctgcaagccg gcgcgctcgt atcgctcacc
    17401 catcacgaga agttcgacgg ctcgggatat cccgacgggc tcgcgggcga ggcgatccat
    17461 ctctacggcc gcatcgtcgc ggtctccgac gtgttcgacg cgctcaccgc gcggcgtcac
    17521 tacaaggagc cgtggacgat cgatgattcg ctggcctatc ttgcgcgcga gtcgggcgcc
    17581 cacttcgacc cgcggctggt ggagcatttc gtcgcgaacc tggacgcggt cctagccgtc
    17641 cgggagcgct acgcggagcc tgccgcctga gcggcccgcc ggcttccgcc ggttgatgcc
    17701 gcatcacccg gtccgatcgc gcgccgtcat tccgtaaaag taaactcgcg cacgaaggac
    17761 tcctgctcgt ccgtctccac ggccccgggg acggagcggc gcacccactc gatcgcctga
    17821 tcgcccgaat atccgcagac ggcgcgcgca aggagcgcga ggacgatgcc ggtgcgcccc
    17881 caaccgccgt agcaatggac ggagatccgc tcgccgcgac cggcgcgacc gacgatctcc
    17941 ccgatgagct cccgcgtcgc ttcgatggaa tacggcacgg agtagttgac gattgggaaa
    18001 tagagcaccg cgaacccctc ccgggcatag cgtgcgcgca agtccacatt gtcgcggcgc
    18061 aggcactcct cgtcgggcag gagcatgacg atcgtggcga tacccgccgc gcggtaggcc
    18121 gcaatcaggc cgccgcccgg atcgaacgag ccgaggggca tggcgctccg gtagatcgcg
    18181 ccgggaagcc caaatggcag ctcgaccagg ctcatcgggg ggcacccttc ggaatcatac
    18241 cccgtccctc cgcttgtcca ttatgattcg ccagcgcgct tcgatggtag cggcgcctcg
    18301 atactcaatg ggcgccgtgc ggaaaaaaat tcaaatacaa ttcaaatatt tcgcaccggc
    18361 cccgtataca ttgcaggggg tgcatgatgt cgaatgtgaa cggggcgttg aacgagcagc
    18421 agctgcgcgc ggccggcggg gagggcgggg tgatgctggt ggtcgccggg gcggggaccg
    18481 ggaagacgcg cacgctggtc gaaaaggtgg gcgcgatcgt ccgtcagggt accgcggccc
    18541 cggagaatat cctcgtgctt accttcagcc gaaaggcggc cgaggagctg cgcgagcggg
    18601 tgcgcgcggc ccttggcgcg tccgcggacg cgataccgat gggcaccttc cattcgtttt
    18661 gcctgggggc cctgcgggag gccgcggacg attacctcgg ctggtcgggc cgcgatcgct
    18721 ttccggcggt gatggacgat gcggcgcgcg ccgggcttct cggggaactg gtcgccgggc
    18781 ggcgggagcg cttcctgggc atccccgacg acgtggtgct ctcgctcatg gaggggacgc
    18841 ggcgggtcga tccgcgcatc gcagcgcggc ttgatgcgct gggcgtctcc gccgagctcg
    18901 agcggctgcg cgcggagttc gatgaacgca agctggcggc gggccttctc gatttcgagg
    18961 acatgatcgg ccatttttcg cgcctcctgc gcgagcgccc ggcggtgcgc cgtcggatga
    19021 ttgaccgcta ccgctacatc ctcgtggacg agttccagga caccgccccg gggaatttcg
    19081 aggtgatcga gctgctgtgc cccgacgttg ggcgaaatct tttcgcggtc ggcgacgacg
    19141 cgcagagcat ctatggcttt cgcggggcgc ggccgggcta cctggtcgag atgcggcgcc
    19201 atttcccgga ggcggcggtc gtgacgctgg agcgcaatta ccgctcgcgc gccgagatcg
    19261 tccgccgttc ggaccgcttc atcgcgcgaa accggggcgg ggtccgaaag cacctcacgg
    19321 cggtgcgcgg cgccgggggc gcggtggtcc cggtcccggc cgggtcgatc cgcgaggagt
    19381 gcgcgcgtct ttcgcagatc gtggagcgcg agcgcgccgc cggcgcgacg atcgccgtgc
    19441 tgtaccgcaa caactggcag ggggaggcgg tccgccgcga gtgccgcgtc gccgatgcgg
    19501 ccggcgtttc gctcatgacc atgcacgcgt ccaaggggct cgagttcgac gcggtgatcg
    19561 tgatcggcgt cgcggacggc atcatcccgg acccggccgc ggacatcgag gaggagcgcc
    19621 ggctcctgta tgtcgcgctc acgcgcgcgc gggaccggct gtacgtgatc catcgcgcca
    19681 acgccgacgg aagcccggcg cgattcgccc gcgagctcgg ctgggggaag cgccgcggtc
    19741 ccgtcgcctg gcttcgcgat gcgatcgcgt cggcgcgcgc cgcgcggggc cgacgccgcg
    19801 aggcgggatc ggaccggacg gtgcgcagtg agatacgggg ggatgcgcaa tgcgaatcgt
    19861 ggtgaagggg aaggccgcaa agcagctgtg caagatcgag aagagcaatc gcgcgctcgc
    19921 gatcaggatc cgaaacaaga tccgccggta cgcggccgat ccgcaggggc gccatgacgt
    19981 gaagcccctg cgcagcggga gcgtttcgac gcgccgcctg cgcgtcggcg tgtaccgcgt
    20041 gacgctcgtc gcgaaggagg aggtgctcta cgtgctggag agccggcacc ggcagggggc
    20101 ctatcgcggc tgatgggtcc gctaggcggt gctcagcttt ttccggaact cgtccgaggg
    20161 gagcggcttg ctgtagtagt agccctggaa ctggtcgcag cccaggtaga gcaggaagtc
    20221 gatctgttcc ttggtctcga cgccctcggc caccaccttg aacccgaggt tgtgcgcgag
    20281 gtcgatgatg gtcgtggcga tgttcaccga ggtcgcgtcg tgcgggaggt cgtccacgaa
    20341 cgacttgtcg atctttatgg tgtcgatggg aagcgacttg agcttgctca gcgacgagta
    20401 gcccgtgccg aagtcgtcga tcgagatgct gacgcccagc gcattgatct cgttgagctt
    20461 gccgatcgac tccttctcgt tctccatgat gccgctctcg gtgatctcga actcgagcca
    20521 ctgcgggtcg agccccgtga ccgagaggat ctccttgatc ttgtcgatga ggcagggctg
    20581 ccggaactgg aaggacgaga ggttcacggc aaccttgagc ggcggcagcc ccatcgtctg
    20641 ccactccttg gtctggcggc acgactcata catgatgatg tagccgatgt ccttgatgag
    20701 gccgttcttc tccgcgagcg ggataaaatg aagcggcggc acgagccccc gcgccggcga
    20761 ctgccagcgg atgagcgatt ccattcccac gatggcgccc tcgcggttca ccttgggctg
    20821 gtagaaggcg cggaactcct ggttgtcgat ggcgaccacc aagtccttct cgagacggag
    20881 gttgtggatg gtctgctcgt gcatggtgtg gtcgtagagg tgataggtgc cggtcccgcg
    20941 ctgcttggcc atgtacagcg ccgcctcgct gttcttgatc agcgcctccg ccttgtcgcc
    21001 gtcgttggga tacaggcaca cgccgatggt catggacacc gtgagctcaa tgccggccgc
    21061 cgtgagcggc gcgcgaaacg cgtcctcggt tttgcgcacg atcgacgaga tgtcgtcggc
    21121 ggtcttgatg tccgacagca gcacgatgaa cttgtcgccc tccaggcggg ccaccaggtc
    21181 gtccgcgcga aaggtcgtct tgagccgctt cgcgatctcc ttgaggagct tgtcgccggt
    21241 gacgggcccg tgcatttcgt tgatgttctt gaatcggtcg gtcccgaggc acatgacgcc
    21301 gaatatgtac tcgttgccgc ggcgcttcga tttcatgatc tcggtgtgca ggcgcgtggc
    21361 gaagacctcg cggttgggca ggcccgtgag gttgtcgaac atcgagtagt acagcgcctg
    21421 ctcctcggcc tgcttgcgct cggtgatgtc ctcgtgcaga aagacgatgc tctgtatcgc
    21481 gccgcccttc tcgaggatgg ggaaggcgcg gcagcgcagg atcatgggcc gtggcgcgcc
    21541 gcccgggcgg tccggcgggc gctcgacggt catctcgggg atgatgacgc tctcgccccg
    21601 gaacgcctcg tcgatgtagt tctgcagtcc gcgggagatc acgggctcgt ggttgcggat
    21661 gttgaatcgc ccgatttgct cttggggatc aagcccccag atggtctccc aggccgtgtt
    21721 cacgtcggtg agaaccccgt cgggggtgta cagcgnnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnntgtac agcgccaggt cgatcatgtg cgtgagcatc gtggtgtcca
    21901 gcggcttggt gatgtagccg taggggtccg tggagagcgt gcgggcgaag gtcgatgcgt
    21961 ccgagagcga ggtgatgtag atgatgggga tccggtgccg ctccttgatg gctgtcgcca
    22021 gctcgatgcc gttcatcgtg ccgccgagaa tgatgtccat gaggatgagg tcgggcggct
    22081 ccgtgcggac gatctccagg gcccgctcgc cggtctcggc gatgccggcg acgcggtagc
    22141 cggattgttc gaggattccc ttgatgtcga gcgcggtgat gggctcatct tcgacgatga
    22201 ggatgccggg tatttgctcc atattcacgg tatcaatcac ggcgggtatt ttttcaataa
    22261 aaatttatat tttgcaattg acagtccgca cgacggcctc gtacggtatc cgggctgcgg
    22321 attcgcttcc cgtttgcagc cgattacgca cggagggctt tatggataga tttatcagcg
    22381 atctgattcg tgaatatcac gtcctgttca agaacatctt cggcatcggc gggctcatcc
    22441 tcgcctggat ctgcatcacc ggttttatcc gcttctgcat cgcggcatgg cggcgaagaa
    22501 agggcgtcta tcctccggcg gccagcatgg aggaccacta gaaatcggag ggtcgggcgc
    22561 catgcccggc cctttttcgt ccggtcccgc ctattcgcgc tccacgccaa ttatcgtaat
    22621 atcgtcgttc agctcctcgt cgccgataaa atcccgcatt gccgcgagca tgcgctcgat
    22681 gatccgctcc gccggttccc ccgcgttcgt gagggccagc cggccgagcc gctcctcgcc
    22741 gaagagttcc ttggtcgcgt tgaactcctc gaagaggccg tcggtgaaca ggatgagccg
    22801 gtcgcccggc ccgaaggcaa cggtccgggt ggagatgttg atgctgtcgt gcagtcccac
    22861 cgcgcgcccg gtccgctgaa gcggcttcac ggcgccgccg cggatgagaa actggtcggg
    22921 atggccggcg ctggcatagg tgatgcttcc gcgcgccgtg tcgatatcgg ccaccacgcc
    22981 ggtgaagaac accacgagcg aggcgtactt ggccatgaat tcgctgttca gcgtttccag
    23041 gagttcggcg gggctggcga tcgcgttctt gagcatctcg tattcgctct tgatgatcat
    23101 ggtcaccagg gccgcctgca cgccgtggcc ggtcgcgtcg gcgatgaaga cgcggagcgc
    23161 gccgccgtcc cgcacggcga tgtcgtacag gtcgccgccg atctcgccca tggggatgta
    23221 ctgtatcgcg aggccgaggt cgccgatacg gtccgcggcg gcgggcagga tgttctgctg
    23281 aatgtgccgc gcgagcgtga ggtccttctt gatgctgtgg taggccaggt ccagcttgtc
    23341 gtaggcgtcc ttgagctcca ggctctgctc ctccactttc tggtaggcct cgttgagctt
    23401 ctggtaggcc gtggtgagct ccgaggtccg ctcgtccacc ttccgctcca ggttgttgta
    23461 gagcgccgag ttttcgatgg cgatggccgc ctgggccatc agcgcctcca ggacccgcgc
    23521 gtcctcgtcg gtgaacacgc cgctggagag ccggttgtcc agatagcaca cgcccaggac
    23581 gcgctcgtgc tgccggatgg ggacgcacag gaccgacttg agcccctgct cgaccacgct
    23641 gtggtactcc cccagcgcgc ggtcggcttc ggcgttgagc gaaatcacgg ttttcgcgtg
    23701 gcggaacacg tccgcgacga tgtggcgcga gtgcgcggct tcgtcggcgt cgggaccggt
    23761 cgcggcccgc acgacgagct cggcgccggt ctcgtcgaca aggaagaggg cgccgttctg
    23821 cgcgccggtc acctcgatcg ccttggcgag gatggtgtcg atgagctcgt cgatgtccag
    23881 gatggagctg atgtcctggc tcaccgtgat gatcgacgag agccgttgcc ggtccatgag
    23941 gcgctgggcg gcgctcggcg cctcgccgcc gcccatcccc aggcgctcct cggtcttctt
    24001 gaggaaaccg gccgcgccga tctcgctgaa gatgcggtag gccgtttcca gccgcttgcg
    24061 cgcggactcg gcgtgacccg tatcggccag gaagcggccg tagtcgtaca tggcgcgcgc
    24121 gagttcatgg cggcggccga tccgctcggc gtggtcgatg ctgcgctgga agagccgccc
    24181 cgcggcgcgg tggttgcccg agagggcctg cgcgcgggcg ctttcccgga gcgcccccgc
    24241 gtggtgcgcc ggccatgatt tcgttttccg gagggcggtc cggcaggcgc gccgtatagc
    24301 gcgcaacgcg cctcggctcc cgcgcccggt tccggcgcgc gcgaggtgct cggcgatcaa
    24361 cacctcggcg taccgggtgt agaggtgcac cgtgtagtgg cgcatgaagt tgtttttctc
    24421 gaagagccgg cgggcggtct cgatgtgctc ccgcgcccgg gcggtgtcgc cctgttcgag
    24481 atacacgccc gcaagctccg tgttcaggat gcagtactgt atccacattc cgtgcgccgt
    24541 gcagaggggg agcgcggccg tcgcggtcga ttcggcgccg gcataatcgc cgcgctcgac
    24601 ggcgtactgc gtccggtaga gcgcccccca gagcaccgag aagaggtcct tgcgccgctc
    24661 gagcaccgcg aagaactcgt cgagataggt catcccgttt tcgtagtcgg acaggtagta
    24721 gtagctttgc aggagtccgt tcaccgactg gttcaagagc agcatgtcgc cgatggtgcg
    24781 cgcgcgcgac aggctctcct cgaagtgctc gatgctcttc cggtattctc ccatccactg
    24841 gtagcagtag ccgatgaact ggtgcgactc gaccagtccc ggctcgtcgc gcagttcgtc
    24901 gcgcatcgcc agcgagcggc ggtgatacgt gagcgcgcgc ccgaagcgcg ggatcgccat
    24961 gcacaggctg gcgtatcccc ccaggctcag gcccagctcc cgcgaggggc cgatgcggcg
    25021 ctggctgatg ttcagcatgc ggagaatgga gcggatgaat ttcaggaggt cgctgtagat
    25081 atacgaccag ttgagcgagt ggtagtaggc gatcatgagc ttgtgccgct cgctccggct
    25141 gtcgcgccgc cggacgaaga ggcgcggcgc gagtacgtgc agggcgtgcg tacagagctc
    25201 gacggcggtg cccgccgcca ccgcgatctt ccccaggggg aggcgttcgc ccagcaggga
    25261 cagcccctcg gccgcggtgc gctcgcagga ggggaagtcg cccttcttcc agtaggcgtc
    25321 gctgatctgg cggatgacgg cggcctgttc gtacttcgcg cccatgcgcg cgcgcaacgt
    25381 cgtgtagagc gcgatggcct cgtcgtgccg gccggtcatg agaaggatgt cggcggtatg
    25441 gcgcaggcac tccagcatga gaggcgagtc cgtgtcgccc gactcggaga ggagcgcggt
    25501 cacggtggtg aaatacttga gcgcctcgtc gttcgcgtac cgccgcatcg cgcgcagccc
    25561 ggcgggatgg gcgaagcgga gggagtgctc ccggtcgccc gcggcgatcc agtgatgggc
    25621 gatctcgaac aacgcgtcat cgccgggctc ggggtggagc tcctcgagcg tggccgcgat
    25681 cttcccgtgc agggcggaga gctccgcggc ggcgatgttc tggtaaaacg cctcgcggat
    25741 gcggtcgtgc acgaagatga tcttcccgcg gtcgttacgg tcctcctcga ggagctgcag
    25801 gcgcaccgcg cggtccacga tggacacgat cgcgggaatg gaataggccg gcatgaggcg
    25861 gaacaggatc tcgatctcga acttgcggcc aatcaccgag gcgtaggaga gaagcgcgat
    25921 gtcgtcgggc gggagcgtgg cgatccgctt cagcaggatg tccaggatgg tgggcgcgat
    25981 ctgcgcgtcc tccaaaacgg cgcggtccac ggaccagccg tgcgcgcccg ggacaagggc
    26041 cttttcgtcc accagctgct tgaggatctc gatggcgaag aagggattcc cgccgctttt
    26101 ggaaagcacc agatcggcga tcccgccgat cgccgggccc gtgtcgaaga gcagcccctc
    26161 gacgaactgc cgcatgcgtg cgcggtcgaa gagctcgaga cgaatgtcgt gcaccggcag
    26221 cgagcgctcg cccgcctgcc ggatgaagcg cgcgagcggt ccctccggcg cgatctcgtt
    26281 gtcgcggtag gtccccacca ccaggagcgg cgcttggtcg attccctgta tgatctccag
    26341 cagcaccgca atcgttccct cgtcgcacca ctgcaggtcg tccaggacga tcaccaggcc
    26401 gcgttcgatc tcgcccaggg tcctgaaaaa ccgcgccacc acggagagaa agcgcgccgc
    26461 ctccttctcc gggtccagcg gcaccggcgt ctctcccgcg ccgatgagcg cggcgcaggg
    26521 aggattgaac tgcgtcacga tctccgagag cgggtcgagc gcggcccgca ggcgggtgcg
    26581 gatgctttcc tggcgcaggg cgctgtactt gccgaaaaag cgcaggtaca cgttcgcggc
    26641 ctccttgagc ggaccgaagg gcggcttgtt ggcgcccgag aaacacttgc cgtcgatgta
    26701 gagcccgttc atgccgcgca cgtaggtgca cagctcctcg atgaggcggg acttgccccg
    26761 ccccgcctcg cccccgacga ggcacacgga gcccgtgccg cgcataacgc cggtgtagcg
    26821 ggcgacgagc gcatcgtatt ccttctcgcg gcccaccagg acggtgcggt agttgagccg
    26881 cgagatgcgg tcgttggtgc ccagaacgaa atcgtcgcgc ccgccgagga agcggtcgag
    26941 gtcggccagc agcccccgcg cgctctgata gcgccgctcc ggctccttcg cgatgagctt
    27001 gcagatgacc tgttccagca cgggcgggat ggtgtcgtcg atggcgcgcg gcggctccgg
    27061 gagccgggcg atgtgctggt ggatgagcgc gctcaccgtg tccccggtga agggcatccg
    27121 ccgcgtgacc aggtggtaga agaggatccc cagcgagtac aggtcgctgc gttcgtccac
    27181 cgcgcgcttg atcacgccgc actgctcggg cgccatgtag ccgaaggtgc ccgcgacctc
    27241 gtcgctgcg
//
LOCUS       DAORHF010000021        27134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1099_length_27134_cov_6.171867, whole genome shotgun sequence.
ACCESSION   DAORHF010000021 DAORHF010000000
VERSION     DAORHF010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 27134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27134
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_27134_cov_6.171867"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /gene="msrB"
                     /locus_tag="PKJ16_03300"
     CDS             complement(<1..415)
                     /gene="msrB"
                     /locus_tag="PKJ16_03300"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007979191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HNV46042.1"
                     /translation="MKNIYSLCIILTFAAILAQCGASSNIDKRGDKPMNYTVTKSDIE
                     WKTILTPEQYRVMREGGTERPFSGPLNDNYRDGTYFCPGCGTALFSSATKYDHRTGWP
                     SFTAPIDRKNIEYRQDTSHGMIRTEVRCAACGAHLG"
     gene            complement(540..1442)
                     /locus_tag="PKJ16_03305"
     CDS             complement(540..1442)
                     /locus_tag="PKJ16_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HNV46043.1"
                     /translation="MKIFITGGSGFFGRTLTSRLRADRHDITIVDRRITDPSAFPAGL
                     TGIEADTTKPGDWQAAAAASEIVVNLAGTPIFNRWSKAIKRSILESRVLTTRNIVEAL
                     ASRKKKTKRLINASAVGYYGFLNDEIVDETFPPGADFLATVARAWEDEAVRAREHAVD
                     VTACRFGIILGKGGGALALLKKIYRLRAGSRLGGGRQWFPWVHEDDLAEMVAFIIKHR
                     ITGAVNCTAPVPVRNAEMTRQLNRALGVFPLVPPAPAFAVKLALGEFGETLLRGQRAF
                     PRVLTERGFEFRYPEFERAIRSLA"
     gene            complement(2055..2279)
                     /locus_tag="PKJ16_03310"
     CDS             complement(2055..2279)
                     /locus_tag="PKJ16_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNV46044.1"
                     /translation="MNYKERVTASPDIMLGKPVIKGTRITVEIILRKLSEGMAVDELI
                     TAYPHIQKEDIMACLSYSAEVISREEILAS"
     gene            complement(2358..3584)
                     /locus_tag="PKJ16_03315"
     CDS             complement(2358..3584)
                     /locus_tag="PKJ16_03315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HNV46045.1"
                     /translation="MKHMNTHPRTILHVDMDAFFAAVEQLDHPEYRGKPVVVGADPMG
                     GHGRGVVSTASYEARRFGIRSAMPISTAYRACPGAIFVQPRGKRYGEVSRQIMEIFES
                     FSPLVEPISIDEAFLDCTGAEGIFGGPKDLGRKIKERIRNQTGLTASIGIASNKSIAK
                     IASDLKKPDGLVICPPGAERDFIAPLPISRLWGAGPKTVKHLESIGFRTIGDIANAPC
                     DTMEKIHGAWGVHLWQLANGLDDRPVDPTWERKSISEERTFMTDTDDRAEIYRAFLEI
                     ADELSRRMRRERLRGRTLTLKIRLEGFITHTRSATLTLGVNDMASLRDTAYALYGRFD
                     RRGKKVRLLGIGLSNLEPDEPREVQLELFPTPDDGGSATEKVRTIERLMDSLRDDLGA
                     RVSRASLIKGRKQHGQ"
     gene            3750..4715
                     /locus_tag="PKJ16_03320"
     CDS             3750..4715
                     /locus_tag="PKJ16_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46046.1"
                     /translation="MFECFLLSSTEKKILKNAHEALGTLKDRMAGNPTVDSKIALAAG
                     FLEHYRNAKEAGRKTGEVETHEFITARAFDLVGVIDGAFRDDVIRHSMDPDRELDLLA
                     IAAEGHFYGPIDAAGSAYGNYMQRVYLPEVLDKIEAVHDIYENALTNFQAHYESCSAG
                     PADAATLGIALHYLQDMTAPHHAGNYALFFSRYTDHFDTHLAFEKYARQLVNRHPDRF
                     TADAADLRTGFAQRFDELGPREFAREINRMIHPFFPMIKKKDRTAWGDVIDAAIPIAI
                     AASAVVLASALSGAKRTRSAGSGVKKKTAATRRSGRKTATTTRRR"
     gene            complement(4784..9943)
                     /locus_tag="PKJ16_03325"
     CDS             complement(4784..9943)
                     /locus_tag="PKJ16_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46047.1"
                     /translation="MNLDHAIISNKYRITGMIGEGGISEVYRAVDIISGNDRIALKVL
                     KKNRTTSRIEDVIRFQGEAAIASHLTHPHIVSVFDVGSLSGLHYLCMEFIDGMSLAEL
                     LRNGSTFTPAEAVDIVAQVARALEHIHRSKIIHRDLKPGNIMISRDGPDGAPIARIID
                     FGLAQIKEFGAITDPDEIAGTFSYMSPEQSGVIKRPVDERSDLYSLGIIFYQLLTRAL
                     PYEGDSITALLHQHVAAVPRPPVSLNPDLPRVLDRIVLKLIEKEPANRYQGAEGLLRD
                     LEAYRRGETDFTVGQQDAHVKLDFRTALVGREREMARLSARLDSMLQGNGGVCLIGGG
                     AGSGKTRIAEEVMDRFAQRQGIVIGGKCFSGDAKTPYGPLRDALGALVKQYGKLSPHR
                     QAALREQLRGSTGELGAVIESLNPLVREILGESQPLIALEPERENRRFLTVAARFFLA
                     LGAIDGGLILFLDDLQWADTGTFAVLSEIAQEIASRPVLVLGTYRDNEISASHPLRAL
                     TKSAQDNHQPLDHVPLGNFDSETLNRFVASLLHMPPDDTREIHAHVQRQSGGNPFFAI
                     EIIKRMIDDGSIAFREMRWVLVPEQIAHTRIAASIIEIILQRITQLTSRDMTILSYAA
                     LIGHTFDMDLLFRLLPADGTAEIVSAIDRAIELRLLEEDSIVKGVVHFVHDRIREACT
                     ERIASDERRRIHLEIARILEGDRADERDERIFELAHHVIEGGDDEKTLAYAVPAGEMA
                     KDEYANAEAIRYFTIARDILERRGRTGDERWRLCVTRIGELLILTGQHDAAIEQLTGL
                     LEHQHAIPEKALTNRLLADAFFKKGDLARCEIYAHDGLALLGERLPLGVTAVVAGIIK
                     ELGIRALHRALPLIFVRKSENPRGNSYRQMLPFFKTLGWNYSLSDTKKYARLIMRVLN
                     LCERRLGPSKELGTALMGYAGIFMFAAWFDTALALHRKAVALARRSNDLWGTAQTLQL
                     TAYCHKWKGEYPAALTLFGESLRIFREIGDIREVGITNAGIGETHLYLGDYAAAKQAN
                     DDFMKISRKTRDHYGVSAALMCSMPYHIQRGELHRAYENGSESYAIAQERDLPFVLCE
                     TSMYLGVLALEQSDTDQAIKYLEQARTLYEKNSFLKHYTVFLYPYLVEARLAEFTRQR
                     DTLAPSQRNAALRRIRTLCDQSLRITKSWATHHGLALRAAGRFWDTAGKPARAERFLL
                     MSIEHHKRLGRTYELGRSLYEYALHLNRHGAAAASRSQMESAHRIFREIAADDYRKRA
                     AGFLGISQDDTGADQRLMDRQRIDAIIKLSQDVSSILNLDSLLEIIMAKAVEVTGAQR
                     GYLFIHDPDTHALTLKASRGLGDAGAIEYSHTVVDTVFHNARALITANARADNRLNTY
                     HSVVTRGLKSILCVPLLHHTNVIGVCYLDNPLLSGVFTEEDADMLDVFMGQAAIAIEN
                     ASLYNNLEREVELRTRELRQRTREVESKSDELELAYVKLNSAYSRMKEDLILAKNIQD
                     NMLPPRVSRSGSLNIFVEYLPLIEVGGDLYDVFERADGSVRILIADATGHGIVAALFT
                     MLIKSEYEKLKDVIDDPSELFERLNTIFFVSYKPLKMFFTGFLADINPMKSTIRYSSS
                     GHPSQYLIRDGELIELPNTGRPVAIWDEALCETRHEEFRPGDRLLLFTDGLFEEFNRA
                     KEQFGESRLRDLIKQNAHRDARELVTSLVRDLMRYIDSAELNDDITIIGIE"
     gene            10148..10657
                     /locus_tag="PKJ16_03330"
     CDS             10148..10657
                     /locus_tag="PKJ16_03330"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HNV46048.1"
                     /translation="MFFVCISAAACSGKRLSPTVVKNVDLARYAGTWYEIARFPHSFE
                     KGLVCVTATYTINPDGAIAVVNAGRLERDRSQRKEARAKAWVPDPAEPGKILVQFFWP
                     FRGNYWIIGLDGAGYRYAVVAGPSPDYLWILSRSKTMDATLYDSLVALARSQGFDVSR
                     LERVDQSCE"
     gene            complement(10926..11585)
                     /locus_tag="PKJ16_03335"
     CDS             complement(10926..11585)
                     /locus_tag="PKJ16_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46049.1"
                     /translation="MMHAHIAVGMAAKRIAPRVSLGTLIFAGIFADLLLSVLLLAGVE
                     HLRIAPGFTAVVPMDLYDYPYSHGLLHSMVWSILLGVGYFAWKRDVRGSIVVALVAAS
                     HWVFDFISHIPDLPLAPGYGARYGLGLWEHYAATHIVEGALFIGGFALYLKTTRPLDR
                     IGRYALAGLFTALCVMMMGSLAGSVPPSATVMAFTTLLQLLLVALAAWGDRHREMRVP
                     A"
     gene            11747..12193
                     /locus_tag="PKJ16_03340"
     CDS             11747..12193
                     /locus_tag="PKJ16_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46050.1"
                     /translation="MAISTTVCMFGDTLERINEIRAKRGMSRNDAIVRLLRRAMQAGA
                     VLMKTGTAVRYQKSVGRGEWHVVHVRFARAEYEFFTDLRKVYKMSVSLLLAYAAMGFL
                     DDGEEGDNYCFCSHDLSFLNQGMMFRWIITWNPTKHSPEKTRKQRE"
     gene            complement(12216..13217)
                     /locus_tag="PKJ16_03345"
     CDS             complement(12216..13217)
                     /locus_tag="PKJ16_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46051.1"
                     /translation="MIEHIGIVIGKFMPLHAGHIALIRFAAERCTRVLISVDGQPDHP
                     IPVDTRAEWIRKTFADDPRIEVHATDEPLPDDPVPSRAVSSIWAEYLKRRFPQATAIV
                     SSETYGDYVAEYMGISHIPFDPDRAANPVSGTAVREHPFRHWHLIAPAARSYFAQRVC
                     IYGPESTGKSVMTEYLARRFKTTFVPEMARELLAERGVAGLVREDFAAIGELHARDIL
                     RRTPEANRILFVDTDHITTKIYARHYFDFTPDFPAWIEEANRYGLYLFLDIDVPWIED
                     PQRDSWHCREEHRALFLGELERAHLPHVVISGSWEERNRKAVAAVLERWPDMEFNGS"
     gene            complement(13214..13888)
                     /gene="pnuC"
                     /locus_tag="PKJ16_03350"
     CDS             complement(13214..13888)
                     /gene="pnuC"
                     /locus_tag="PKJ16_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HNV46052.1"
                     /translation="MQALFDITHTLFTAWGYKVSPLELLGTSTGLACVWLTAREKVLC
                     WPVGIANIVFFFVLFYQHRLYSDMFLQVYFLATSLYGWWKWTHPAPAEENRRHELRIT
                     RLRAGRLALIAAGCAIAVAALGTLVGRLHAIVPAVFPEPAAFPYADTAVAVASVAAQF
                     IMARKKIECWLFWIGVDILATAIYFLKGINLLAIEYAVFGCIALYGFLGWRREMRSYE
                     TEGAPA"
     gene            complement(13894..14376)
                     /locus_tag="PKJ16_03355"
     CDS             complement(13894..14376)
                     /locus_tag="PKJ16_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HNV46053.1"
                     /translation="MTSWRQQATEGIAPGMTVRFSRTFTQEETNTFGDITRDYNPVHY
                     DERFAKQKGFTDLICHGLLVGGMVCELGGQVGWLATGMKFLFKRPVYFGDTVTCEITV
                     RTVDENGFAVADARFTNQSGDEVLEAKISGLVPAAQDRALLATMISEGDPTNKIAGER
                     "
     gene            complement(14455..15810)
                     /locus_tag="PKJ16_03360"
     CDS             complement(14455..15810)
                     /locus_tag="PKJ16_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV46054.1"
                     /translation="MEHNTRTPLMKPEWLATIHRMRKHPDAPRWNIEIGDRLTAEDLA
                     YAKSFGETLAADRARPASSPTDDIVAWIEERRERVPYFARVLDGIDARRDFGAIPRMT
                     RRDLQRNLDSIVPPDEPLDRLIVNPTAGTTGEPILAPNHPRAIGCYDPLIQFCLRRHG
                     VRARYDHTVIAAIQACFQKSTIVYTAVHSFLDGAGFAKINLDPSAWPRPDSAARYVRD
                     MAPVFISGDPIAFMEMLRLGVEYRPQALLSCALALTGDLRARLESAYGCPVVDFYSLN
                     ETGPLAYSSPERPGCFHVLPHDIHLEICDDAGLPVPDGAVGEICMTGGRNPFLPLLRY
                     RTGDTGRFVSRNGTAGDPSPLIELCDCRMPVLFSTPDGRSINPIDIARIIRSHPVESF
                     QCVQASDLAVTVRLPRASGAHFGEEISRRIADLFGGTVAVRHTALVVHSEEKIIPFIK
                     E"
     gene            complement(15885..16601)
                     /locus_tag="PKJ16_03365"
     CDS             complement(15885..16601)
                     /locus_tag="PKJ16_03365"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HNV46055.1"
                     /translation="MNDTSTHETAPNIRVAVIEPGGPVNVGSIARAMANFAFDDLVLV
                     GNDVDDPDGRGARVAMGGAGVFAGRRIAGTLDAALEGCGLVIGATRRARSDESCIDFR
                     EAARIAARRPRAATVALLFGRERDGFMRQERERCHILAEIPSVEGTAGSLNISHAAAL
                     FLCAIYCELGGPEPVAHADPEPLLAAFDDLFKRSGTWEANGRAQRIFHGLLARAGLTP
                     EEAELLERSFRLFGARIQPE"
     gene            complement(16790..17395)
                     /locus_tag="PKJ16_03370"
     CDS             complement(16790..17395)
                     /locus_tag="PKJ16_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46056.1"
                     /translation="MQTLHKAVSTFSRFAKLAALAIAASTLVFGLIHLASLSLLAETG
                     RIVIEHDALLHLLSVNMLPMVMAYLFLVAVLYYLYRMARAAIAQSAKEEIESARREAT
                     VATLQKVTGVIAEELVAPNTELRRYIELKKRKGQAPPALETASGSISTTLRRLTWLSY
                     VLPHLETDATGDDALTIIANAPAHTTGNPLLLPGPVAKRRQ"
     gene            complement(17564..18142)
                     /locus_tag="PKJ16_03375"
     CDS             complement(17564..18142)
                     /locus_tag="PKJ16_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46057.1"
                     /translation="MKKSRKLIVDKKYQFGFAAPLTAASGAVITLVIVAIAAVSLVNN
                     GKLHELASNQMRLVANQNDIYQTLLAVSQAKDLSNPSVVEEFVNRDVARNKVRLDDNT
                     AIIIGIGRNNLILISGLIVFALLQSGILFYLLIKRSHRISGPVLVLNRYIDDIKNGTF
                     PDVRPLREKDDLKELFENFRQMVEALKRKGGG"
     gene            18331..18921
                     /locus_tag="PKJ16_03380"
     CDS             18331..18921
                     /locus_tag="PKJ16_03380"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46058.1"
                     /translation="MNAKPGRRDEIMDASLRLIERKGIQNLTTKNIAREVGFTEPGIY
                     RHFASKEDILAAILERFRSVVNDRNRAILDGDMDPLDRIGAMYANTIGYFAENPSVTA
                     AVFAEEIFQNSSRLSATVRRIMDRTQRALRTTIRLGQERGCVRGDLPDEQLALIVMGS
                     FRLLVTRWRLSGRSFNLVREGAKLWETVRAMLSAER"
     gene            18944..19522
                     /locus_tag="PKJ16_03385"
     CDS             18944..19522
                     /locus_tag="PKJ16_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HNV46059.1"
                     /translation="MNATDELQKEHRFILAMIEIIGELCNRLERDGTADPGHLAAVID
                     FIRTFADGCHHAKEERALFPALEAAGIPRENGPVGVMLAEHDAGRRFVTAMDATLDGI
                     RAGDAAAARDFIVNARGYMELLVKHIHKEDEVLFVLADRYLSPDEQERLVREFERIER
                     DETGAGAHERYHALLHELRGVYLDGRPPGTRT"
     gene            19559..20752
                     /locus_tag="PKJ16_03390"
     CDS             19559..20752
                     /locus_tag="PKJ16_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HNV46060.1"
                     /translation="MAIQVKNNVWWVGKTDWELRKFHGSEYSTHRGTSYNSYLVREEK
                     TALIDTVWRPFADEYVDNLAKTIDLSKIDFVVANHAEIDHSGALPELMERIPEAPVYC
                     TANGVKSLKGHYHRDWNFVPVKTGDRISLGSKELIFIEAPMLHWPDSMFCYLTGDNIL
                     FSNDAFGQHYATSGLFNDLADPAELFAECVKYYANILTPFSPLVLKKIAEFVSLSLPL
                     DIICTSHGVIWRDNPMQIVERYLAWADAYRENRIAILYDTMWNGTRVLAEQMAKGVVE
                     ADPSVTVELFNVATTDKNDVITEVFKSKGIMVGSPTINRSVLSSVAAILDQIHGLQFK
                     GKKAAGFGCYGWSGESVTDINERLGKAGFDVMPDGFKALWNPDEKSRDDAVRFGRSFA
                     EFCRG"
     gene            complement(20763..21374)
                     /locus_tag="PKJ16_03395"
     CDS             complement(20763..21374)
                     /locus_tag="PKJ16_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46061.1"
                     /translation="MTATGPSIAHLTHRLANAPAAVTNEPASAPDMPEVLTTAAMISD
                     MIDGLGGGLLTMREATAVTALRTKAGDRIMLMLQAAAFLAADPWFADYARAHPDADLA
                     AKTKSFLMNGLTELSKYVKPDYLVGDPEGREELARRYLAALDLVPEGETAAQAADRLT
                     ALDSVERARLIGATRESQKRARELREAMARREAEEAASKMTRE"
     gene            complement(21371..23137)
                     /locus_tag="PKJ16_03400"
     CDS             complement(21371..23137)
                     /locus_tag="PKJ16_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV46062.1"
                     /translation="MKTKAPEHTRSDRVLLAERWRERWPEALGLWGSFVRLPEPIFCQ
                     DTAQARAEGLTESFAMIRLTDHRIVLSPMTILSLGIGEYALEIMGHEIGHHVYCPADM
                     SDTGRMIARIRRALPTMEQYAPMVGNLYTDLLINDRLFRQHGLRMDAVYRAMKAPHAD
                     ALWTFYLRIYEILWGLQKGTLVEGETSTELEADARLGNRVVRSYANDWVRGAGRFAAL
                     CLTYLLAEKAGTSEALRAILDAQVTACGDEIPDGLAEMDDGEEGDSIHPALEDDEAEE
                     KRPTQPGGPQAVRGNYREPFEYGQILRAMGITVSDEDIAVRYYRERARPHLVPFPVRV
                     EPRASDPMPEGLDQWDIGEPIDGINWIETAMRAPVVVPGVTTVQTRYGEERGFEKAVT
                     PIDLDLYVDCSGSMPDPRQATSYLALAGTIIALSALRAGSRVQATLWSDTGGFQTTNG
                     FVSDERLIMTVITGYIGGGTAFPIHILRDTYANRKPTDRTVHILCISDDGITTMYDND
                     ERGNSGRPIAANALARAAGGGSLVLNLYGDWRNNKELLFASEQGWAIYPVSTWEDLVA
                     FSREFVRRNYLAHKTSRGVMQP"
     gene            complement(23134..24189)
                     /locus_tag="PKJ16_03405"
     CDS             complement(23134..24189)
                     /locus_tag="PKJ16_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46063.1"
                     /translation="MAGLIPPELAACVERHRVALNAAFAAHRAGHPRIDADGVLRAFA
                     SLAETARAALADRPDAIDALIEPLFAISLELAGPRGRLPGLEAHLGRFIALFPHAVSH
                     EPDRLLRDAANAYRTLFARCVSAPERWTALMAGTSRLAPTPDELRRYGAIAAWRAGLA
                     EFRQAAVASIGASGSDAVRALLGISDNVAMPDPAAFAAALADNPWADPAALAAGSAQP
                     RACFAAIGGFSAFGGVFAAPPVVFAQDGIALAHDGTRCFEIIADRFGAHFAPAGAAPG
                     DRIAPDPSVVVGPSAIVAHGISIALRDIAPIDANHPLPDLPVVPVRGSAYDGHAVYLT
                     TPLSYRVFVVGIAGAGA"
     gene            complement(24177..25754)
                     /locus_tag="PKJ16_03410"
     CDS             complement(24177..25754)
                     /locus_tag="PKJ16_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009957996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46064.1"
                     /translation="MDVPKQFTKVSESPTLPGKRGARKTFDVADLYRDPSGEPAPDAG
                     LGLDEKLRKAYFWITNSAIITPFYDIEFNDGPAPEYTVGDSKSIVRLPSAQSYSSFVL
                     LPLLTLAVRGKCLFVGGPGRGKTASAIIMGVLAGYPLIDIKRAIQHGQPQMTIADLLG
                     NPLPADLMKAESMDAIRIAWRKWLAMRVKIIDEYNRIPTRTQSALLTVMADNYAEIMD
                     QVYECPDAAWYLTANDDAGGGTYQVIEALRDRIDVVVHALHFNSRFIGELLARVDSGV
                     KPETLVPGEIIFTGEEIDRMHADILAVEMPRALLRRLEHFASQFEFCEPAGEQIEYKT
                     KDVVKLAGENPATLAERETGKSKAKDLGLQTANGLSVRAIMTILVFTKALAYFRGARE
                     VSPDDLRAMVPFVLHDKLVQNPDASFLEEQGNAVYRVDRVSWIRRLFDLSCAEYDQVG
                     LDKNDPVGALMDAFARGLEGVTRKQAEEALVAIERRMDAHQKERKLHGPIFDDILALK
                     YLHQRYRNYLRWLEWQG"
     gene            25925..26401
                     /locus_tag="PKJ16_03415"
     CDS             25925..26401
                     /locus_tag="PKJ16_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46065.1"
                     /translation="MVTPTDTFGDGLKEKGASMSRSETELEALVEEWKRGSWRESGIV
                     DEIAARSAGAPPESPLEELIAQRRALETDRDAVQDLISARRSEELQGSDLEAVIEEKR
                     RDELDKTPLEEIIEMRRSEEALVSGFQEQYLAAMNAREFEKTNLELIIEVHRAGIV"
     gene            complement(26479..>27134)
                     /locus_tag="PKJ16_03420"
     CDS             complement(26479..>27134)
                     /locus_tag="PKJ16_03420"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV46066.1"
                     /translation="HPMRMIGGDIIDFLRFRDPDTIGVFIGDVSGHGVPAALVAAMLK
                     GVINQSGDSRSDPSRFLLYLNDCMFDTTGGYFITALYGIFTVSTGRFAFSAAGHPPPV
                     LVRGDDASFYDMPGSVPLGIMTTADLLDFDKGYAAHAIDIRAGETLLLYTDGLYEACP
                     ANGDGSPQFGERALLDTLRAARGLPGTELIRAVLDTAVAHRGSDQFDDDVSMICIEG"
BASE COUNT         4757 a         8170 c         9115 g         5092 t
ORIGIN      
        1 gtcccaggtg ggcgccgcag gcggcgcagc gcacctcggt gcggatcatg ccgtgcgagg
       61 tgtcctgccg gtattcgatg ttcttccggt cgatgggcgc ggtgaagctg ggccatccgg
      121 tccggtggtc gtacttggtc gcggagctga acagcgcggt gccgcagccg gggcaaaaat
      181 aggtcccgtc gcgatagttg tcgttgagcg gcccgctaaa gggacgttcg gttccgccct
      241 ctcgcataac gcggtattgt tccggcgtga gtatcgtttt ccactcgatg tcagattttg
      301 ttacggtata gttcatgggt ttgtcccccc ttttgtcgat gttcgaactc gcgccgcact
      361 gtgcgaggat ggcggcgaac gtcagtatga tgcacaagct gtatatgttc ttcatggtgg
      421 tgtcctttag gtttgtttac ctcaccccgc cttcggcgcg ccctctccca ttggagaggg
      481 tttcgatgta aaagactaac tcctgcggga gaaggggtcg ggggatgagg tgtgccgtct
      541 cacgccagac tccgtatcgc ccgctcgaat tccggatagc ggaactcgaa cccgcgctcg
      601 gtgaggacgc gggggaacgc ccgctggccg cgcaggagcg tctcgccgaa ctcgcccagg
      661 gccagcttca ccgcgaaggc gggggccggc gggacgagcg ggaagacgcc gagcgcgcgg
      721 ttgagctgtc gcgtcatctc cgcattgcgc accgggacgg gcgccgtgca gttgaccgcg
      781 cccgttatgc gatgcttgat gatgaacgcg accatctccg cgaggtcgtc ctcgtgcacc
      841 caggggaacc actggcggcc gcctcccagc cggctcccgg cgcgcagccg gtagatcttt
      901 ttaaggagcg cgagggctcc gccgcccttc cccaggatga tgccgaagcg gcacgccgtg
      961 acgtcaacgg cgtgctcgcg cgcgcgaacg gcctcgtcct cccaggcgcg cgccaccgtc
     1021 gcgaggaagt ccgcgccggg agggaaggtc tcatcgacga tctcgtcgtt caggaagccg
     1081 tagtacccca cggccgaggc gttgatgagc cgcttcgtct ttttcttccg cgacgcgagc
     1141 gcctccacga tgttgcgcgt ggtgaggacg cggctttcga ggatcgagcg cttgatcgcc
     1201 ttcgaccagc ggttgaagat gggcgtcccc gcgaggttga cgacgatctc gctcgccgcc
     1261 gcggccgcct gccagtcgcc cggcttcgtc gtgtcggcct cgatcccggt gaggcccgcg
     1321 ggaaatgcgg acggatcggt gatccgccgg tccacgatgg tgatgtcgtg cctgtccgcc
     1381 cgcaggcgcg acgtgagcgt gcgcccaaag aaccccgaac cgccggtgat gaagatcttc
     1441 attgccgcct ccctcgcttt cgattggaac agtatagcgc atacgccatc ggtttgccaa
     1501 ggaaattggc ggcacgcgcg tatggcgccg gccgaagtca ttacgcaggg tatctgtgag
     1561 gcggtcgaag ttccggtata gagcagaggc gtcacggagc acgtcatatc gcttgctgtg
     1621 ctgtcggacg ttacgttatg cgtgaaggtc gagaatcctt gagatggatc tcgagcgttt
     1681 cacgaaagaa aagaatggcg aatttacaag tcgcgaattc gaattttttt tgtagttata
     1741 acgacaaact ttccgcgtaa tgtatctttg tgacgccgta ttaggtttaa caatacttcc
     1801 cctatggcgt tttcttccat gtgattgtat cgcaataata ttacgcttaa atgttccgtt
     1861 ttgtgggcaa acactcattc tccgaaatcc ttgtcctcgg taagcacgat ggcattgttt
     1921 ttaatggcga gctcaatgat tttagatcgc ttaagccgcg gtgggtttcg taaattgaca
     1981 atacctcaat ggagttctgc tttagtaatg aaataatttc acaatcgatg ttctcatcgg
     2041 cgataatggc aatatcagga tgcaagtatt tcctcccgac tgatcacctc ggcggaatac
     2101 gaaaggcaag ccatgatgtc ctctttttgg atatgcggat aggccgtgat aagttcatcg
     2161 accgccatgc cctcggataa ttttctcaga atgatttcga cggttatccg cgtacccttg
     2221 ataaccggct tgccgagcat gatgtcggga ctcgctgtta cccgttcctt gtagttcatt
     2281 atcattaccc cgtcgtacaa tctgacaata cggtagtatt gaagcatgct tctgtcaaca
     2341 catttcgcca tcctgcctta ttgtccatgc tgctttcgcc ctttgatcag ggaggcacgg
     2401 gagacgcgcg cgccgagatc atcgcggagg ctgtccatga ggcgctcgat ggtgcggacc
     2461 ttttccgtcg ccgagccgcc gtcgtcgggt gtgggaaaaa gctcgagctg aacttcccgc
     2521 ggctcgtcgg gttcgaggtt cgacaggccg atccccagca ggcgcacctt ttttccccgg
     2581 cggtcgaagc ggccgtacag cgcgtaggcc gtatcgcgga ggctcgccat gtcgttcacg
     2641 cccagcgtca gggtcgcgct tcgcgtatgc gtgatgaagc cctccaggcg tatcttgagc
     2701 gtcagggtgc gtccgcggag ccgttcgcgc cgcatccgcc gcgacagctc gtcggcgatc
     2761 tcgaggaagg cgcggtagat ctccgcgcga tcatcggtgt cggtcatgaa ggtgcgctcc
     2821 tcgctgatgg acttgcgctc ccaggtcggg tccacggggc ggtcgtccag cccgttcgca
     2881 agctgccaca ggtggacccc ccaggccccg tggatcttct ccatggtgtc gcacggtgcg
     2941 ttcgcgatgt cgccgatcgt gcggaagccg atcgattcga ggtgcttcac cgtcttgggg
     3001 cccgcgcccc agaggcggga gatgggcagc ggtgcgatga agtcccgctc ggcgcccggc
     3061 gggcagatga ccagcccgtc cggtttcttg aggtccgatg cgatcttcgc aatggacttg
     3121 ttcgaggcga tcccgatgct ggcggtgagg ccggtctggt tcctgatgcg ctccttgatt
     3181 tttcttccga ggtccttcgg gccgccgaag atcccctcgg cgccggtgca gtccagaaac
     3241 gcctcgtcga tgctgatggg ctcgacgagc ggcgagaagc tctcgaagat ttccatgatc
     3301 tgccgcgaca cctcgccgta gcgctttccg cgcggctgca cgaagatcgc gccggggcag
     3361 gcgcggtatg ccgttgagat gggcatggcg gagcggatac cgaaccgtcg cgcctcgtag
     3421 ctcgcggtcg acaccacgcc gcgcccgtgt ccgcccatcg gatcggcgcc cacaacgacg
     3481 ggcttgccgc gatactccgg atggtcgagc tgctcgacgg ccgcgaagaa ggcgtccatg
     3541 tcgacgtgaa gaatggtgcg gggatgcgtg ttcatgtgtt tcatttgccc aatcggacta
     3601 tacaaatata tagtattcgg gttttttgtc aatcacattt gcgatgccga tcgctcctgt
     3661 gcgacgctgg accgtgcggg tcaaaatcga acttgacgcc gttcgcatca aatttgttat
     3721 tcaataaaaa acacaccgga gattgtgaca tgtttgaatg cttcctgttg agttcgacgg
     3781 aaaagaaaat actgaaaaat gcgcatgaag cgctggggac gctcaaggac cgaatggcgg
     3841 gcaaccccac ggtcgattcc aaaatcgcgc tcgcggcggg cttcctcgag cattaccgga
     3901 acgccaagga ggcgggcaga aaaacgggcg aggtggagac ccacgagttc atcacggcgc
     3961 gcgccttcga cctggtcggc gtgatcgacg gcgcgtttcg cgacgacgtt atccggcact
     4021 cgatggatcc cgaccgcgag ctcgatcttc tggcgatcgc ggccgagggg catttctacg
     4081 ggcccatcga cgcggccggg agcgcgtacg gcaattacat gcagcgggtg tacctgccgg
     4141 aggtcctcga caagatagag gcggtgcacg acatttacga gaacgcgctc acgaacttcc
     4201 aggcgcatta cgaatcatgc agcgccggtc cggcggatgc cgcgacgctc gggatcgccc
     4261 tccactacct gcaggacatg acggccccgc accatgccgg caactacgcg ctcttctttt
     4321 ctcgatacac cgaccacttc gacacgcacc tggcgttcga gaagtacgcc cggcagctcg
     4381 tgaaccggca ccccgaccgc ttcaccgcgg acgccgcgga cctgcgtacg ggattcgcac
     4441 agcgttttga cgagctgggg ccgcgcgaat ttgcgaggga gataaaccgg atgatccacc
     4501 cgttcttccc catgatcaag aaaaaggacc ggacggcgtg gggcgacgtg atcgacgccg
     4561 cgataccgat cgccatcgcg gcgtccgccg tggtcctcgc gagcgcgctc tcgggagcga
     4621 agcggacgcg atcggccggg tctggcgtga agaagaaaac ggcggcaacg cggcgttccg
     4681 ggagaaagac ggcaacgaca acgcgccgtc gttgacgagg cccatcgcac gttgtttcaa
     4741 tgcgctcacc ggcaacgatt tagtaagagc ggacgccgtc ccgtcactca atgccaatga
     4801 tggtgatgtc gtcgttgagt tccgctgagt cgatgtaacg catcaggtcc cggaccagcg
     4861 aggtgacgag ctcacgtgcg tcgcgatgcg cgttttgttt gatgaggtcc cggagccggc
     4921 tctcgccgaa ctgctccttg gcccggttga attcctcgaa gagcccgtcc gtgaacaaca
     4981 gcaggcggtc gccgggacgg aattcttcgt gcctcgtctc gcatagcgcc tcgtcccaaa
     5041 tcgccacggg acgaccggtg ttgggaagct cgatgagctc cccgtcgcgg atgaggtact
     5101 gtgacggatg tccagatgat gaatagcgga tcgtgctctt catcgggttg atgtcggcca
     5161 ggaagccggt gaagaacatc ttgagcggct tgtacgatac gaagaagatc gtgttcagcc
     5221 gctcgaagag ctcgctcgga tcgtcaatca cgtccttgag tttctcgtat tcgctcttga
     5281 tgagcatggt gaacagagcc gcgacgatgc cgtgaccggt cgcgtcggcg atcaggatcc
     5341 ggaccgatcc gtccgcacgt tcgaatacat cgtacagatc gccgcccacc tcgatgaggg
     5401 gcaggtactc gacgaagatg ttcagggagc cggagcggct gacgcgcggc gggagcatgt
     5461 tgtcctgaat gtttttggcg agaatgaggt cttccttcat tcgcgagtat gcgctgttca
     5521 gctttacgta cgcgagctcg agttcgtccg acttggattc cacctcccgc gtccgttgcc
     5581 gcagctcccg ggtccgcaac tccacctcgc gctcaagatt gttgtacagg ctcgcgtttt
     5641 cgatggcgat ggcggcctgg cccatgaata cgtccagcat atcggcgtcc tcttccgtga
     5701 acacgcccga gaggagcggg ttgtcgaggt agcagacgcc gatgacgttt gtgtggtgga
     5761 gcaacggtac gcagaggatc gacttgagac cgcgcgtgac gacgctgtgg tatgtgttga
     5821 gcctgttgtc ggcgcgtgcg ttcgcggtga tcagcgcgcg ggcattgtgg aacaccgtgt
     5881 ccacgacggt gtgggagtat tcgatcgccc ccgcatcccc cagcccgcgg gaggccttga
     5941 gcgtcaacgc atgcgtgtcg ggatcgtgaa tgaacaggta cccgcgctgc gcgccggtca
     6001 cctcgacggc cttcgccatg atgatctcga gcagcgaatc gaggttgagg atggagctca
     6061 cgtcctggct gagcttgatg attgcgtcga tgcgctggcg gtccatcagc cgctggtcgg
     6121 cccccgtgtc gtcctgcgaa atccccagaa aacccgccgc gcgtttgcgg tagtcgtccg
     6181 cagcgatctc cctgaagatg cgatgcgccg actccatctg cgagcgcgag gccgctgccg
     6241 cgccgtgccg attgaggtgg agcgcgtatt cgtagaggct gcggccgagt tcataggtgc
     6301 gtccgagccg tttgtggtgc tcgatgctca tcaagaggaa tcgctcggcc cgcgccggtt
     6361 tgcccgcggt atcccaaaaa cgccccgccg ccctcagggc cagcccgtga tgggtcgccc
     6421 aggatttcgt tatccgcagt gactggtcac ataacgttcg tatccgccga agcgccgcgt
     6481 tccgctggga aggcgccagg gtgtcgcgct gccgggtaaa ctccgccagc cgggcctcga
     6541 ccaggtatgg atagaggaag acggtgtagt gtttgaggaa gctgtttttt tcatacaggg
     6601 ttcgcgcctg ttcgagatat ttgatcgcct ggtcggtgtc ggactgctcc agggcgagga
     6661 cgccgaggta catcgacgtc tcgcacagga cgaacggcag gtcccgctcc tgggcgatgg
     6721 cgtagctctc gctgccgttc tcataggcgc ggtgcaattc gccccgttgg atatgatacg
     6781 gcatgctgca catgagcgcc gcgctcacgc cgtagtggtc gcgggtcttg cgcgagattt
     6841 tcatgaaatc gtcattggcc tgtttcgccg ccgcgtaatc gccgaggtag aggtgagtct
     6901 cgccgatgcc cgcgttggta atgcccacct cgcgtatgtc tccaatttcg cggaatatgc
     6961 gcaggctctc cccgaagagt gtgagcgccg ccgggtattc gcccttccac ttgtggcaat
     7021 aggcggtcag ttggagcgtt tgcgccgttc cccacaggtc gttcgatcga cgcgccagtg
     7081 cgaccgcctt tcggtgcagg gcgagcgcgg tgtcgaacca cgcggcgaac atgaatatgc
     7141 ccgcatagcc catcagcgcc gtgccgagct ctttggaggg gcccagccgg cgctcgcaca
     7201 gattgagcac ccgcatgatg agtcgcgcat acttcttcgt atcgctgagc gagtagttcc
     7261 agccgagcgt tttaaagaac gggagcatct gtcgatacga gttcccgcgt gggttttcgc
     7321 ttttacgaac aaatataagc ggtaatgcgc ggtgcagcgc ccggatgccg agctccttga
     7381 tgatgcccgc caccacggcg gtaacgccta aagggaggcg ctcgcccagg agcgcgaggc
     7441 catcgtgcgc atagatctcg caccgggcca ggtcgccttt tttgaaaaac gcatcagcga
     7501 gcagccggtt ggtgagggcc ttttccggga tggcgtgctg atgctccagt agccccgtga
     7561 gctgctcgat cgccgcgtcg tgctgcccgg tgaggatcag cagctcgccg atgcgcgtca
     7621 cgcacaatcg ccagcgctcg tcgcccgtcc ggccgcgccg ctctaggatg tcccgcgcga
     7681 tcgtgaaata gcggatcgcc tcagcatttg cgtattcgtc tttcgccatc tcgccggccg
     7741 ggacggcgta ggcgagcgtt ttttcgtcgt cgcccccctc gatgacgtga tgggccagct
     7801 cgaagatgcg ctcatcgcgc tcgtccgcac ggtcgccttc gagtatgcgg gcgatttcca
     7861 ggtggatccg ccgccgttcg tccgaggcaa tacgctcggt gcacgcctcc cgaatccggt
     7921 catggacgaa atgcacgacg cccttgacga tgctgtcctc ctcgagcagc cgcagctcga
     7981 tcgcccggtc gatggcgctg acgatttcgg cggtgccatc ggcgggcagc agccggaaga
     8041 gcaggtccat gtcgaaggtg tgcccgatga gcgccgcata cgagaggatg gtcatgtcgc
     8101 gcgaggtgag ctgcgttatc cgctgcaaga tgatctcgat gatggacgcc gcgatgcggg
     8161 tgtgcgcgat ctgctccggg accagcaccc atcgcatctc gcgaaatgcg atgctcccat
     8221 cgtcgatcat ccgcttgatg atctcgatag cgaaaaacgg attgcccccg ctctgacgct
     8281 gcacgtgggc gtgaatctcc cgcgtgtcgt cgggcggcat gtgcaggagg ctggcaacaa
     8341 agcggttgag ggtctcgctg tcgaaattcc ccagggggac atggtcgagc ggctggtgat
     8401 tgtcctgggc gcttttcgtg agcgcgcgca gcggatggga cgcggaaatc tcgttgtcgc
     8461 gataggtgcc cagcaccagc acgggccgcg atgcgatctc ctgggcgatc tcggagagca
     8521 cggcgaaggt ccccgtgtcg gcccactgga ggtcgtcgag aaacaggatg aggccgccgt
     8581 cgatcgcgcc cagggctagg aagaaccgcg cggccaccgt gaggaagcgg cggttctcgc
     8641 gctcgggctc cagcgcgatc agcggctggc tctcgccgag gatctcccgg acgagggggt
     8701 tgagggattc gatgacggcg cccagctcgc cggtcgaccc gcgcagctgt tcccgcaacg
     8761 cggcctggcg atggggcgaa agctttccgt actgtttcac gagcgccccc agcgcgtcgc
     8821 ggagcgggcc gtacggggtc ttggcgtccc cggagaagca cttgccgccg atgacgatcc
     8881 cctgccgttg cgcgaaacgg tccatgacct cctccgcgat tcgggtcttt cccgatccgg
     8941 cgccgccgcc gatgaggcag acgccgccgt ttccctggag catgctgtcc agccgcgcgg
     9001 agaggcgggc catctcccgc tcgcggccga ccagcgcggt gcggaaatcg agctttacgt
     9061 gcgcgtcctg ctgtcccacc gtgaagtcgg tctcgccgcg ccggtaggcc tccaggtcgc
     9121 gcagcagccc ctccgcgccc tggtagcggt tcgcgggctc cttctcgatg agcttgagga
     9181 cgatccggtc caatacgcgg gggagatcgg gattgagcga cacgggggga cgggggacgg
     9241 cggccacgtg ctgatgcagc agggcggtga tgctgtcgcc ctcgtagggg agcgcgcggg
     9301 tcagcagctg gtaaaagatg atccccagcg agtacaggtc gctgcgctcg tcgaccgggc
     9361 gcttgatgac gccgctttgt tcgggggaca tgtagctgaa ggtcccagcg atctcgtccg
     9421 gatcggtgat ggcgccgaat tccttgatct gcgccaggcc aaagtcgatg attctggcaa
     9481 tcggcgcgcc gtccggcccg tcgcgcgaga tcatgatgtt gcccggcttg aggtctcgat
     9541 gaatgatctt gctgcggtgg atgtgctcca gcgcgcgtgc gacctgcgcg acgatgtcca
     9601 cggcctcggc gggtgtaaac gtcgagccgt ttcgcagcaa ctccgccagg ctcatgccgt
     9661 cgatgaattc catgcacagg taatggagcc cgctgagcga gcccacatcg aacaccgaaa
     9721 cgatatgggg gtgcgtgagg tgcgaggcga tcgcggcctc accctggaag cggatcacgt
     9781 cctcgatgcg gcttgtggtc cggttcttct tgaggacttt cagggcgatg cggtcattgc
     9841 cgctgatgat atcgaccgcc cgatagacct cgctgatgcc gccctcgccg atcatcccgg
     9901 tgatgcggta tttgttcgag attattgcat ggtccaggtt cattgcgctc gcgctctgtc
     9961 atagtattaa tccagctggt cggaaatcaa gccaatatcg tcggcgacgg gtaaaaatcg
    10021 tgttgcgaaa tgcagacggt tgcgctatca atagtgccgg cggggtaacg ggtgcggtcg
    10081 atgctccgca acaactgctg ggtgctgagg gggggatatg cgatgttgtg gacgttttct
    10141 tgtgttgatg tttttcgtgt gcatctcggc tgcggcctgt tccgggaagc gcctcagccc
    10201 cacggtcgtg aaaaacgtgg atctcgcgcg ctacgccggg acctggtacg agatcgcgcg
    10261 cttcccgcac tcgttcgaga agggcctcgt atgcgtcacc gcgacctaca cgatcaatcc
    10321 cgacggcgcc atcgcggtgg tgaacgccgg gcgtctggaa cgcgaccggt cgcagcgcaa
    10381 ggaggcgcgc gcgaaggcct gggtgcccga tccggccgag cccggcaaga tcctggtgca
    10441 gttcttctgg cccttccgcg gcaactactg gatcatcggg ttggatggag ccggctatcg
    10501 ctatgccgtc gtcgcgggcc cgtcgccgga ctacctctgg atcctttcgc gaagtaaaac
    10561 gatggacgca acgctgtatg attcgctcgt tgcgctcgcg aggtcccagg gattcgacgt
    10621 gtcgcggctc gaacgcgtgg accagagttg cgagtgattg tgacgcgtgc gtggctgcag
    10681 gatgcgccgg ggcgttatgt gctttactgg agaccgtgca ctggtttcgc gatttcccgg
    10741 cctcgcggtc accgcagcgc agttcgtggg ccgttcgcga tagcggtatt atcttcttgt
    10801 tgcgatagca ctttctctat gttctcaatc cagcggcttg tgcggcgact ctgccatttg
    10861 tgacggcggt gtgcgttctc gcgaactgtt gagtcgagcg gaacgcacca tccatgtcac
    10921 tcgtctcacg ccgggaccct catctcccga tgccggtccc cccacgcggc gagcgcgacc
    10981 aggagaagct gcaatagcgt cgtgaacgcc attaccgtcg cgctgggcgg cacgcttcct
    11041 gcgaggcttc ccatcatcat cacgcacagc gccgtgaaca atcccgcaag cgcataccgg
    11101 ccgatccggt ccaatggccg cgtcgtcttc aaatacagcg cgaatccgcc aatgaaaagc
    11161 gcgccttcca cgatgtgcgt ggcggcgtaa tgctcccaga gccccagccc gtaacgtgcg
    11221 ccatagcccg gcgcgagcgg caaatcggga atgtggctga tgaagtcgaa gacccagtgg
    11281 cttgccgcga cgagggcgac gacgatcgat ccgcgcacgt ctcgcttcca ggcgaaatag
    11341 ccgacgccaa gcagaatcga ccagaccatc gagtgaagaa ggccgtggga gtaggggtag
    11401 tcgtacaggt ccatcgggac gacggcggtg aatcccgggg cgatccgcag atgctcgaca
    11461 ccggcaagca ggagaacgct gaggaggaga tcggcgaaaa tcccggcgaa gatgagcgtc
    11521 cccagcgata cgcgcggcgc gattcgcttt gcggccatcc cgacggcgat gtgtgcgtgc
    11581 atcatggcgg tattctcctt cctgtggctg ctgatgtttc gagcgtattt gctcctgtgt
    11641 aatgacgatc ggaatttttt ccataagaaa acgggtccat gcaggaaaaa catcaaacac
    11701 tcgtctgtgc ggcgcgtata tacatatatg acgcgaggag gcgggcgtgg cgatttccac
    11761 tacggtgtgc atgttcggcg ataccctgga gcgaatcaat gaaattcggg caaaacgggg
    11821 gatgtcccgg aatgatgcga tcgtcaggct tctcaggcgg gccatgcagg cgggcgctgt
    11881 gctcatgaag accgggaccg ccgtgcgcta ccagaaaagc gttggtcgcg gcgagtggca
    11941 tgtcgtccat gtgcggttcg ctcgggcgga gtatgagttt tttacggatt tgcggaaggt
    12001 gtataagatg tcggtgtcat tgcttctggc gtatgcggcg atgggttttc ttgatgatgg
    12061 ggaagagggt gataactatt gcttctgttc acacgatctt tcatttttaa accagggaat
    12121 gatgtttcga tggattataa cctggaaccc aacaaaacat tcccccgaaa aaacccggaa
    12181 acagcgcgaa taaccgcatc actcgcaaag gaaagctagc tcccgttgaa ttccatatcc
    12241 ggccatcgct caagcacggc cgcgaccgcc ttccggttgc gctcctccca ggatccgctg
    12301 atgacgacat gcggaagatg cgcgcgctcg agctcgccga gaaagagcgc gcgatgttcc
    12361 tcgcggcagt gccaggagtc ccgctgcggg tcctcaatcc acggaacgtc gatgtcgagg
    12421 aacaggtaga gtccgtaccg gttcgcctcc tcgatccatg cggggaagtc cggcgtgaaa
    12481 tcgaagtagt gccgcgcgta gattttcgtg gtgatgtggt cggtgtccac gaagaggatc
    12541 cggttcgcct cgggcgtccg gcggaggata tcgcgcgcgt ggagctcgcc gatggccgcg
    12601 aagtcctcgc gcacgaggcc ggcgacgccg cgctcggcga ggagctcgcg cgccatctcc
    12661 ggcacgaatg tcgtcttgaa acggcgcgcg aggtattccg tcatcacgct ctttccggtg
    12721 ctctcgggcc cgtagatgca cacccgttgt gcgaagtatg atcgcgccgc gggtgcgatc
    12781 aggtgccaat ggcggaaggg gtgctcgcgg acggcggtgc cggatacggg attcgcggcg
    12841 cggtccggat cgaacggaat atgcgagatc cccatgtatt cggccacgta gtcgccgtat
    12901 gtttctgatg agacgatggc cgttgcctgc gggaaccttc gtttcaaata ctccgcccag
    12961 atcgatgaca ccgcgcgcga ggggaccgga tcgtccggga ggggctcgtc ggtggcatgg
    13021 acctcgatgc gcgggtcgtc cgcaaaggtc tttcggatcc actccgcgcg ggtgtcgacg
    13081 gggatggggt ggtcgggctg tccgtccact gagatgagta cccgcgtaca gcgctccgcc
    13141 gcgaaacgga tcagcgcgat gtgccccgca tggagcggca tgaacttgcc gatgacgatc
    13201 ccgatatgtt cgatcacgcc ggcgctccct cggtttcgta cgatcgcatc tcacgccgcc
    13261 aacccaggaa gccgtagagc gcgatacagc cgaagaccgc gtactcgatc gcgagcaggt
    13321 tgatgccctt gaggaagtag atcgcggtgg cgagtatgtc cacgccgatc cagaacagcc
    13381 agcactcgat cttcttgcgc gccatgatga actgcgccgc gacgctcgcg acggccacgg
    13441 ccgtgtcggc gtaggggaag gcggccggtt ccggaaaaac cgcagggacg atcgcgtgaa
    13501 gccgtcccac cagcgtcccg agggcggcca ccgcgatcgc gcacccggcg gcgatgagcg
    13561 cgaggcgccc ggcgcgcagt cgcgttatgc gaagctcgtg gcggcggttc tcctcggccg
    13621 gagcggggtg cgtccacttc caccagccgt acaggctcgt cgcgagaaag tacacctgga
    13681 gaaacatgtc cgagtacagg cggtgctggt agaagagaac aaagaaaaaa acgatattgg
    13741 cgatccccac cggccagcag aggacctttt cccgcgccgt gagccacacg caggccaggc
    13801 cggtggacgt ccctaggagc tcgagcggag aaaccttgta gccccaggcg gtgaagagcg
    13861 tgtgagtgat gtcgaagagg gcctgcatcc cgcctagcgc tcgccggcga tcttgtttgt
    13921 cggatcgccc tcggagatca tggtcgcgag gagtgcgcgg tcctgggcgg cgggcacgag
    13981 cccggagatc ttcgcctcga gcacctcgtc gcccgattgg ttcgtgaagc gcgcgtcggc
    14041 cacggcgaag ccgttctcgt ccacggtgcg cacggttatt tcgcaggtga cggtatcccc
    14101 gaagtatacc ggtcgtttaa agaggaactt catgccggtg gccagccagc ccacctgtcc
    14161 gcccagctcg cacaccatgc cgcccacgag cagcccgtgg cagatgaggt ccgtaaatcc
    14221 cttctgcttc gcgaagcgtt catcgtagtg cacgggattg tagtcgcgcg tgatgtcgcc
    14281 gaaggtgttc gtctcctcct gcgtgaatgt acgtgaaaag cggaccgtca tgcctggggc
    14341 gatcccctcg gtcgcctgtt gtctccaaga cgtcatgtgt cgctgtccct ggtcgattgt
    14401 cgatgcacgc gatgcgtgcc tgaccgctca tatcgcgaag gcgcgaaaag acaatcactc
    14461 ttttatgaag gggatgattt tttcctcaga atggacgacg agcgcggtat ggcgaaccgc
    14521 gaccgtgccg ccgaataaat cggcaatgcg gcgggagatc tcttcgccga agtgcgcgcc
    14581 cgacgcgcgt gggagccgca ccgtgacggc gaggtccgat gcttgcacgc actggaacga
    14641 ttccacgggg tgcgaacgga taatgcgcgc gatgtcgatg gggttgatcg accgtccgtc
    14701 cggcgtcgaa aacagcaccg gcatgcggca gtcgcaaagc tcgatgagcg gcgacgggtc
    14761 gcccgcggtg ccgttacgcg acacgaagcg cccggtgtcg ccggtccgat agcgcaacag
    14821 cggcaggaag gggttccgcc cgccggtcat gcagatctcg ccgacggctc cgtcggggac
    14881 cgggaggccc gcatcatcgc atatctcgag atggatgtcg tgggggagga cgtgaaaaca
    14941 ccccggccgc tccggcgacg aataggccag ggggccggtc tcgttgagcg agtagaaatc
    15001 caccacgggg catccgtagg ccgactcgag gcgcgcgcgg agatcgccgg tcagcgcgag
    15061 cgcgcaggaa aggagcgcct gcggccgata ctcgacgccg agtctgagca tctccatgaa
    15121 ggcgatgggg tcgccggaga taaagaccgg cgccatgtcg cgcacatacc gcgcggcgct
    15181 gtcggggcgc ggccacgcgg acggatcgag atttatcttc gcgaagcccg ccccgtcgag
    15241 gaaggagtgc acggcggtat agacgatggt gctcttctgg aaacatgcct ggatcgcggc
    15301 gatcaccgtg tggtcgtagc gcgcgcgcac gccgtgccgc cgcaggcaga actgaatgag
    15361 cgggtcgtag caaccgatgg cgcgcggatg gttgggcgcg aggatgggct cgccggtggt
    15421 gccggcggtg gggttcacga tgagccggtc gaggggctcg tccggcggga cgatcgaatc
    15481 aagattgcgc tgaaggtcgc gccgcgtcat gcgcggtatc gcgccgaaat cgcgcctcgc
    15541 gtcaatgccg tcgaggacgc gggcgaagta cgggacgcgc tcccggcgct cctcgatcca
    15601 cgcgacgata tcgtcggtcg gcgaggaggc gggacgggcg cggtcggccg cgagggtttc
    15661 cccgaacgat ttcgcatagg cgaggtcttc cgcggtgagc cggtcgccga tctcgatgtt
    15721 ccagcgcggc gcgtcgggat gcttgcgcat gcggtggatc gtggcgagcc actccggttt
    15781 catcaggggc gtgcgggtgt tgtgttccat gcttctgcga ttgtcgccga atcgggatcg
    15841 tcctgtcaat cgaatcgtgg aaatttgctt cctcatgacc gcgctcactc cggttgtatc
    15901 cgcgccccaa agaggcgaaa tgaccgctcc agaagctccg cctcctccgg ggtgaggccc
    15961 gcgcgggcga ggaggccgtg aaagatcctc tgcgccctcc cgtttgcctc ccacgtgccg
    16021 cttcgcttga agagatcgtc gaatgccgcg agaagcggct cgggatcggc gtgcgccacc
    16081 ggttcgggtc cgccgagttc gcaatagatg gcgcacagga agagcgccgc cgcgtgggag
    16141 atgttgagcg agccggcggt cccctcgacg ctgggtatct ccgcgaggat gtggcagcgt
    16201 tcgcgctcct ggcgcatgaa cccgtcgcgc tcccgaccga aaagaagcgc gacggtcgcg
    16261 gcgcgggggc ggcgtgcggc gatgcgcgcg gcctcgcgaa agtcgatgca tgattcgtcg
    16321 ctgcgcgcgc gtcgggtcgc gccaatcacc aggccgcagc cctccagcgc cgcatcgagc
    16381 gtgccggcga tacggcgtcc ggcgaagacg ccagcgccgc ccatggcgac gcgcgcgccg
    16441 cgcccgtcgg gatcgtccac gtcgttgccc acgaggacga gatcgtcgaa ggcgaagttc
    16501 gccatcgcgc gcgcgatcga gccgacgttc accgggccgc ccggctcaat caccgccacg
    16561 cgaatgttgg gcgcggtttc atgtgtcgat gtgtcgttca tcgggggaag ggtgatacgc
    16621 gtcgtgtgaa atgtcaatcc aagtccgcgc cgttttccgg acgatgggaa aagccgtcac
    16681 atgcgaatgg cgctccgctg gcgcgcgggg tagcctgccg gcgctgatca gggaaaaacg
    16741 ctgtaatgcg gattgtcgcg gtttcgccga aaatggcgtt atgcgagatt cattggcgcc
    16801 gtttcgcgac gggtccaggc aggaggagcg ggttgcccgt cgtgtgcgcg ggggcgttcg
    16861 cgatgatagt gagcgcatcg tcgccggtcg cgtcggtttc gagatggggc aggacatagc
    16921 tcagccaggt gagccgccgc agggtggtcg atatgctgcc gctggccgtc tccagcgcgg
    16981 gcggcgcctg gcccttccgt ttcttgagct cgatatagcg cctgagctcc gtgttggggg
    17041 ccacgagctc ctcggcgatg acgccggtca ccttctggag cgtggcgacc gtcgcctccc
    17101 ggcgggccga ttcgatctct tccttcgcgg actgtgcgat cgccgcgcgc gccatgcggt
    17161 acagataata caggaccgcc acaaggaaga ggtaggccat gaccatcggg agcatgttca
    17221 cggacaacag gtggagcagc gcgtcgtgtt caatgacgat ccggccggtt tcggccagga
    17281 gggaaaggct cgccaggtgg atgagtccga acaccagcgt ggatgcggcg atcgccagcg
    17341 cggccagttt agcgaaacgg gaaaacgtgg aaacggcctt gtgtaatgtc tgcatgcagc
    17401 ctccggtttc ctgtgggatc gttcgaacgc acgttgcgta ttggggcctg tttcagcagg
    17461 tggatgtccc tactggatag gcttgtgtat atgaggatcc gcgtgcgcat tttgttacag
    17521 gattgcggtc gcttccgcgt catctccgat cgttcaggcg tcgtcagccg ccgcccttcc
    17581 gtttcagggc ctcgaccatc tggcggaagt tttcgaagag ttccttcagg tcgtctttct
    17641 cgcgcagcgg gcgcacgtcg gggaaggtcc cgttcttgat gtcgtcgatg tagcggttga
    17701 ggacaagaac cggccccgag atccggtgcg atctcttaat aagaagatag aacaaaatcc
    17761 ccgactgcag cagggcgaag acgatcaggc cggagatcag gatgaggttg ttcctgccga
    17821 tgccgatgat gatcgcggtg ttgtcgtcga gcctgacctt gttgcgcgcc acgtcacggt
    17881 tcacgaactc ctcgacgacc gaggggttgg acagatcctt cgcctgcgat acggcgagca
    17941 gcgtctgata gatgtcgttc tggttggcga ccagacgcat ctgattgctc gccagctcgt
    18001 ggagcttgcc gttgttcacc aaggagaccg cggcgatggc gacgatgacg agcgtgatga
    18061 cggccccgga ggccgccgtc agcggggcgg cgaagccgaa ctggtatttc ttgtcgacaa
    18121 tgagctttct gctttttttc attctgggcc tccggtcctg attgcgatcg atgttgggcg
    18181 agtatgcgaa accgccgcgc ttcgtgtcat gcagaaaatc tgtcccccgc cgctcccgtc
    18241 gaaaaaatcc cgccggatat ttcatgaaaa aagtggacga accggcgcgc cgatggtata
    18301 ttagtattta ctaacatttg ggggtcgggg atgaacgcaa agcccgggcg gagggatgag
    18361 atcatggacg cctcgctgcg gctcatcgag cggaagggca tccagaacct gacgaccaaa
    18421 aacatcgccc gcgaggtggg tttcaccgag ccggggatct accggcactt cgcgagcaag
    18481 gaggacatcc tcgccgccat tctcgagcgc ttccggtcgg tcgtcaacga tcgaaaccgc
    18541 gccatcctgg acggcgacat ggacccactc gaccggatcg gcgcgatgta cgcgaacacg
    18601 atcgggtatt tcgcggagaa cccgtccgtc acggcggcgg tgttcgccga ggagatcttt
    18661 cagaacagct cccgcctgtc cgcgacggtg cggcgtatca tggatcgcac gcaacgcgcc
    18721 ttgcgcacga ccatcaggct cggccaggag cgggggtgcg tcaggggcga tctgcccgac
    18781 gaacagcttg cgctcatcgt gatgggctcg ttccggctgt tggtgacgcg ctggcggctc
    18841 tcggggcgct ccttcaatct tgtacgggag ggggcgaagc tctgggagac cgtgcgcgcc
    18901 atgctgtcgg cggagcgttg aggagagaag gaggtgcatg gcaatgaacg cgacagacga
    18961 gttgcaaaaa gagcaccggt tcatactggc gatgatcgag atcatcggcg agctctgtaa
    19021 ccggctcgag cgggacggga cggccgatcc ggggcatctc gccgcggtca tcgattttat
    19081 ccgcacgttc gcggacgggt gccaccacgc gaaggaggag cgggccctgt tcccggccct
    19141 cgaggcggcg gggattcccc gcgagaacgg acccgtgggc gtgatgctgg ccgagcatga
    19201 cgcgggcagg cggttcgtga cggcgatgga cgcaaccctc gacgggatac gggccggtga
    19261 tgccgcggcc gcgcgcgatt ttatcgtgaa cgcccgcggg tatatggaac tgctcgtaaa
    19321 gcatattcac aaggaagacg aggtcctgtt cgtcctggcc gatcggtact tgtcgcctga
    19381 cgaacaggag cggctcgtgc gcgaatttga acgcatcgag cgcgacgaga ccggagcggg
    19441 ggcgcacgag cgctatcatg cgctgttgca tgagttacgc ggcgtctatc tcgacgggcg
    19501 gccgcccgga accaggacgt gacggcgcgc agcgcgatca acatacagga ggacggacat
    19561 ggcgattcag gtgaagaaca atgtgtggtg ggtcggcaag accgactggg agctccggaa
    19621 attccacggc agcgagtatt ccacgcaccg ggggacctcg tacaattcct acctggtgcg
    19681 cgaggagaag acggcgctca tcgacacggt gtggcggccg ttcgccgacg aatacgtcga
    19741 caacctcgcg aagacgatcg atctttcgaa gatcgatttc gtcgtcgcca accacgccga
    19801 gatcgaccac agcggcgcgc tgcccgagct catggagcga ataccggagg cgcccgtcta
    19861 ctgcacggcc aatggcgtga agtcgctgaa ggggcattat catcgcgact ggaacttcgt
    19921 gccggtgaag accggggacc ggatctcgct gggttcgaag gagctcatct tcatcgaggc
    19981 gccgatgctg cactggccgg acagcatgtt ctgctatctc accggcgaca atatcctctt
    20041 cagcaacgac gccttcgggc aacactatgc cacgagcggc ctgttcaacg acctggcgga
    20101 cccggcggag cttttcgcgg aatgcgtcaa gtattacgcg aacattctca cccccttcag
    20161 cccgctcgtc ttgaagaaga tcgcggagtt cgtgtcgctg tcgcttccgc tcgacatcat
    20221 ctgcacgagc cacggggtca tctggcgcga caatcccatg cagatcgtcg agaggtatct
    20281 cgcctgggcc gacgcctatc gggagaaccg gatcgcgatc ctgtacgaca cgatgtggaa
    20341 cggcacgcgc gtcctcgcag agcagatggc gaagggcgtc gtcgaggcgg acccgtccgt
    20401 cacggtggag ctctttaacg tggcgacgac cgacaagaac gacgtgatca ccgaggtctt
    20461 taaatcgaag gggatcatgg tcggctcgcc gacgatcaac cgcagcgtgc tgtcgtctgt
    20521 ggcggctatc ctcgatcaga ttcacggcct gcagttcaag ggcaagaagg ccgcgggctt
    20581 cggctgctac ggctggagcg gggagtccgt cacggacatc aacgagcggc tggggaaggc
    20641 cgggttcgat gtgatgcccg acggattcaa ggcgctgtgg aaccccgacg agaagagccg
    20701 cgatgacgcc gtgcgcttcg gccgctcgtt cgcggagttt tgccggggct gatccgggag
    20761 cgctactccc gcgtcatctt cgacgccgcc tcctcggcct cgcggcgggc catcgcctcc
    20821 cggagctcgc gggcgcgctt ctgcgattcg cgggtggccc cgatgaggcg cgcgcgttcc
    20881 accgaatcga gggccgtgag gcggtcggcg gcctgcgccg ccgtctctcc ctcgggaaca
    20941 agatcgagcg ccgccaggta tcggcgcgcg agctcctcgc ggccctcggg atcgcctacg
    21001 agataatccg gttttacata cttagagagc tcggtaagcc cgttcatgag aaaggatttc
    21061 gtcttcgcgg caaggtccgc gtcgggatgc gcgcgcgcgt aatcggcaaa ccagggatcg
    21121 gccgcgagga aggccgcggc ctgcagcatc agcatgatcc ggtcgcccgc cttcgttcgt
    21181 agcgcggtga ccgccgtggc ttcgcgcatc gtgagaaggc cgccgcccag cccatcgatc
    21241 atgtcggaga tcatcgcggc ggtggtgagg acctccggca tgtcgggcgc ggaggccggc
    21301 tcattcgtca cggccgccgg cgcgttcgcg agccggtggg tgaggtgcgc gatggacggt
    21361 ccggtcgcgg tcatggctgc atcacccccc gtgacgtttt gtgcgcgaga taattccgcc
    21421 gcacgaactc cctgctgaag gcgacgaggt cctcccatgt cgagacggga tagatggccc
    21481 agccctgctc gctggcgaag agaagctcct tgttgttgcg ccagtcgccg tagaggttga
    21541 gcacaaggga gccgccgccg gcggcgcgcg ccagggcgtt ggccgcaatc gggcggccgg
    21601 agttcccgcg ctcgtcgttg tcgtacatgg tggtgatgcc gtcgtcggag atgcacagga
    21661 tgtggaccgt gcggtccgtc ggcttccggt tcgcataggt gtcgcgcagg atgtggatgg
    21721 ggaaggcggt cccgccgccg atgtagccgg tgatcaccgt catgatgagc cgctcgtcgg
    21781 agacgaagcc gttggtggtt tgaaaaccgc ccgtgtcgct ccagagcgtc gcctgcacgc
    21841 gcgatcccgc tcgcagcgcc gagagcgcga tgatggttcc cgcgagcgcg aggtaggagg
    21901 tcgcctggcg cgggtcgggc atggagcccg agcagtcgac gtagaggtcg aggtcgatcg
    21961 gcgtgacggc cttctcgaag ccgcgctcct cgccgtagcg cgtctggacg gtggtcacgc
    22021 ccggcaccac gacgggggcg cgcatcgccg tctcgatcca gttgatgccg tcgatcggct
    22081 cgccgatgtc ccactggtcc aggccctccg gcatggggtc ggacgcgcgc ggctccaccc
    22141 ggacgggaaa ggggaccagg tgcggacgcg cgcgctcgcg gtagtagcga acggcgatgt
    22201 cctcgtcgct tacggtgatg cccatcgcgc ggaggatctg cccgtactcg aagggctcgc
    22261 gatagttgcc ccgcaccgcc tggggaccgc cgggctgcgt tggacgcttc tcctcggcct
    22321 cgtcgtcctc tagcgcggga tggatggaat cgccctcctc gccgtcgtcc atctcggcca
    22381 ggccgtcggg gatctcgtcg ccgcacgcgg tgacctgcgc gtcgaggatc gcgcgcagcg
    22441 cctccgatgt ccccgccttc tccgcgagga ggtaggtgag gcagagcgcg gcgaagcgcc
    22501 cggcgccgcg cacccaatcg ttcgcgtagc tgcggaccac gcggttcccg aggcgcgcgt
    22561 cggcctccag ctcggtggag gtctccccct cgacgagcgt ccccttctgc aggccccaga
    22621 ggatctcgta gatgcgcagg tagaaggtcc acagcgcgtc ggcgtggggc gccttcatgg
    22681 cgcggtagac cgcgtccatg cgaaggccgt gctggcggaa caggcggtcg ttgatcagga
    22741 gatcggtgta gaggttgccc accatcgggg cgtactgctc catcgtcggc agggcgcggc
    22801 ggatgcgcgc gatcatgcgg ccggtgtcgc tcatgtcggc agggcagtag acatgatggc
    22861 cgatctcgtg tcccatgatc tccagcgcgt actcgccgat gcccagggag aggatcgtca
    22921 tgggcgagag cacgatgcga tggtccgtca ggcgtatcat cgcgaaggat tccgtgagcc
    22981 cctcggcgcg cgcctgcgcg gtgtcctggc agaagatcgg ttcgggaagg cgcacgaagc
    23041 tgccccagag gccgagcgcc tcgggccagc gctcgcgcca gcgttccgcg aggagtacgc
    23101 ggtcgctgcg cgtgtgttcg ggcgccttcg tcttcatgcg cccgccccgg cgatgccgac
    23161 gacaaatacg cggtacgaga gcggcgtggt gagatagacg gcgtgtccgt cgtatgcgga
    23221 accgcgcacc ggcactacgg gcaggtcggg gaggggatga ttggcatcga tcggcgcgat
    23281 gtcgcgcagg gcgatggaga tgccgtgcgc gacgatggcc gacgggccga cgacgacgga
    23341 cgggtccggc gcgatccggt cgccgggcgc cgcgcccgcc ggtgcgaagt gcgcgccgaa
    23401 ccggtcggcg atgatctcga agcatcgcgt cccgtcgtgg gccagagcga ttccgtcctg
    23461 cgcgaacacg accggcggcg cggcgaacac cccgccgaat gcggagaaac cgccgatcgc
    23521 ggcgaagcag gcgcgcggct gcgccgatcc ggcggcgagc gccgcggggt ccgcccaggg
    23581 attgtcggcc agcgccgccg cgaacgcggc gggatcgggc atggcgacgt tgtccgaaat
    23641 gccgaggagc gcccgcacgg cgtcggaccc gcttgcgccg attgatgcga cggccgcctg
    23701 ccggaactcg gcgaggccgg cgcgccatgc cgcgatcgcg ccatatcggc gcagctcgtc
    23761 gggcgtcggg gcgaggcgcg atgtcccggc catgagcgcg gtccatcgct ccggcgcgga
    23821 cacgcacctg gcgaagagcg tgcgataggc gttcgccgcg tcgcgcaaca agcggtccgg
    23881 ctcgtgagag acagcatggg gaaagagcgc gatgaagcgg cccaagtgcg cctcgaggcc
    23941 ggggagccgg ccgcggggtc cggcgagctc gagcgagatg gcgaagagcg gttcgatgag
    24001 cgcgtcgatc gcgtccggcc ggtctgcgag cgccgcgcgg gccgtctccg cgagggacgc
    24061 gaatgcacgc aggacgccgt cggcgtcgat gcgcggatgg cccgcacgat gggcggcgaa
    24121 cgcggcgttg agcgcgaccc gatggcgctc gacgcaggcc gcgagttccg gcgggatcag
    24181 ccctgccact cgagccaccg gagatagttg cgatagcgct ggtgcaggta cttgagcgcg
    24241 aggatgtcgt cgaagatggg gccgtggagc ttgcgttcct tttggtgcgc gtccatccgc
    24301 cgctcgatcg cgacgagcgc ctcctcggcc tgctttcgcg taacgccttc cagcccgcgc
    24361 gcgaaggcgt ccatgagcgc gcccacggga tcgtttttgt cgagccccac ctggtcgtac
    24421 tcggcgcagg agaggtcgaa gaggcggcgg atccagctca cgcggtccac gcggtacacg
    24481 gcgttcccct gctcctcgag gaagctcgcg tcggggtttt gcaccagctt gtcgtggagc
    24541 acgaacggga ccatcgcgcg caggtcgtcg ggcgagacct cgcgcgcccc gcggaagtag
    24601 gcgagcgcct tggtgaagac gagtatcgtc atgatggcac gcaccgagag gccgtttgcg
    24661 gtctggaggc ccaggtcctt cgccttgctc ttgccggtct cgcgctcggc gagcgtcgcg
    24721 ggattctcgc cggcgagctt caccacgtcc ttcgtcttgt actcgatctg ctcgccggcc
    24781 ggttcgcaga actcgaactg cgacgcgaag tgctccaggc gccgcaagag cgcgcgcggc
    24841 atctccacgg cgaggatgtc ggcgtgcatg cggtcgatct cctccccggt gaaaatgatc
    24901 tcccccggca cgagcgtctc cggcttcacc ccggaatcga cccgcgcgag aagctcgccg
    24961 atgaagcggc tgttgaagtg gagcgcgtgc accaccacgt cgatccggtc gcgcagcgcc
    25021 tcgatcacct gataggtccc gccgcccgcg tcgtcgttcg cggtgaggta ccacgccgcg
    25081 tcggggcact cgtagacctg gtccatgatc tcggcgtagt tgtcggccat caccgtgagc
    25141 agcgccgact gcgtgcgcgt ggggatgcgg ttgtactcgt cgatgatctt cacgcgcatc
    25201 gcgagccact tgcgccaggc gatgcggatg gcgtccatgc tctcggcctt catgaggtcg
    25261 gcgggcaggg ggttgcccag caggtcggcg atggtcatct gcggctggcc gtgctggatg
    25321 gcgcgcttga tgtcgatgag cggataaccc gcgagcacgc ccatgatgat cgcgctcgcc
    25381 gtcttcccgc gcccgggccc gcccacgaag aggcacttgc cgcgaaccgc gagggtgagc
    25441 agcggcagca gcacgaagct cgagtagctc tgcgcggagg gcaggcgcac gatcgatttg
    25501 ctgtcgccca cggtgtactc gggcgcaggg ccgtcgttga actcgatgtc gtagaagggc
    25561 gtgatgatgg cggagttggt gatccagaag tacgccttgc gcagcttctc gtccagcccc
    25621 agcccggcgt caggggccgg ctcgccggag gggtcgcggt agaggtcggc cacgtcgaag
    25681 gtcttgcgcg cgccgcgctt ccccggcagc gtgggcgatt cggaaacctt ggtgaattgc
    25741 ttcgggacat ccatgggcgc ctcccggtat caaacttcgc ttgcccgagt tggaatgatc
    25801 gtgcgcactt gaagaaagtc aattagattt ccatgcacgg cggcatgtct actatgcccg
    25861 ttggaattac ttgaatctat ccgcgtgctt ccgtatcttt tcagtggaga gaaaggagga
    25921 cgccatggtg acgccaactg atacattcgg agacggattg aaggaaaaag gcgcatcgat
    25981 gagcaggagc gagaccgagc tcgaggccct cgttgaggag tggaagcgcg ggtcctggcg
    26041 cgaatcggga atcgtagatg aaatcgccgc ccggagtgcc ggcgcgccgc cggaatcgcc
    26101 gctcgaggaa ctcattgcgc aacggcgcgc gctggaaacg gaccgagacg cagtacagga
    26161 tcttatcagt gcgcgtcgaa gcgaagagct gcagggctcg gacctcgaag cggtgatcga
    26221 ggaaaagcgc cgggatgagc tggataaaac tccgctcgag gagatcatcg agatgaggcg
    26281 aagcgaggag gcgctcgttt ccgggttcca ggagcagtac ctcgcggcca tgaacgcacg
    26341 ggagttcgag aagacgaacc tcgagctgat catcgaggtg catcgggcgg ggattgtgtg
    26401 agatacttgt acccgtcctc gcgcaacatg atgtgaaaaa cggatgtcga tggttgtccg
    26461 tatggtgatg cgccggcgtc atccctcgat gcagatcatg ctcacgtcgt catcgaactg
    26521 gtccgagccg cgatgcgcga cggccgtgtc gagcactgcg cgaatgagct cggtcccggg
    26581 cagcccccga gcggcgcgca acgtgtcgag aagcgcgcgc tccccgaact ggggcgaccc
    26641 gtcgccgttc gcgggacagg cctcgtagag cccgtcggtg tacagcagga gcgtctcccc
    26701 cgcgcggatg tcgatcgcgt gcgcggcata gcccttgtcg aagtcgagca ggtcggccgt
    26761 ggtcatgatc cccagcggta cgctgcccgg catgtcgtag aacgacgcgt catcgccgcg
    26821 aaccaggacc ggagggggat gccccgccgc tgagaacgcg aaccgtcccg tcgataccgt
    26881 gaatatcccg tagagcgcgg tgatgaaata cccgccggtc gtgtcgaaca tgcagtcgtt
    26941 gaggtagagc agaaatcggg agggatccga acgggagtcc cccgactgat tgatgacccc
    27001 tttcagcatg gcggccacca gcgcggccgg gaccccgtgg cccgagacgt cgccaatgaa
    27061 cacgccgatc gtgtcggggt ctctgaatcg caggaaatcg atgatgtccc cgccgatcat
    27121 ccgcatcgga tggt
//
LOCUS       DAORHF010000022        26958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1108_length_26958_cov_5.020555, whole genome shotgun sequence.
ACCESSION   DAORHF010000022 DAORHF010000000
VERSION     DAORHF010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26958
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1108_length_26958_cov_5.020555"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            201..1022
                     /locus_tag="PKJ16_03425"
     CDS             201..1022
                     /locus_tag="PKJ16_03425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46067.1"
                     /translation="MKRVLSAIILSVVLIAAGCNQQDLFDLTSKAQDLKTSGRLPSDV
                     AGLALWLKADGITPLPDGTVISDWPDASGNGITTVVFSNPRYFHTAFNGMPAVRFDGS
                     SYILASEFMNFSSFTIFYVGAYTGPGGGRHIIYDQGVTAGSTPFIGLHISTVSITGTV
                     GDSQATPEFLSITSPLTPTTPHIAVFVLNGQNASLFLNGASSGGTLNLAYDSSTTWFN
                     GPSAPSIGSQNGVMYYQGDIAEIIVYNNALSETDGFAVDCYLSKKYGIAVSHPCD"
     gene            complement(1026..2072)
                     /locus_tag="PKJ16_03430"
     CDS             complement(1026..2072)
                     /locus_tag="PKJ16_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006802643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV46068.1"
                     /translation="MKVLLTGSAGFIGHHVARRLSALGCEVVGLDSINDYYDPALKMA
                     RLRASGIDDNAISYGALVRSSALPGHRFIWLDLCDGDRLRTLFEDERFDAVCHLAAQP
                     GVRHSLIRPASCVESNINGFAVMLEACRAAPVRHLVFASSSSVYGLGGRAPYSPHGPA
                     DHPISLYAATKRANELMAHAYSHLYGIPTTGLRFFSVYGPWGRPDMAYFSFTKRIIDG
                     DAIELFGDGGLRRDFVYIDDVVDAVVAVLERPPLPDGAWDGADPALSAAPYRIYNVGS
                     GASVSIEEFVRAIEHALGATATVIATPRQPGDMLATRADIADLAREFGYCPRVPFAEG
                     LARFVAWYREYYRA"
     gene            2345..3592
                     /locus_tag="PKJ16_03435"
     CDS             2345..3592
                     /locus_tag="PKJ16_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HNV46069.1"
                     /translation="MNRALRILCVSALAVAFITGDTNVSFSQYGKKYKEAVQRQREVQ
                     QKDLAEVRQQVEAIRQQIKENHWKFKAEMTEALKYRIEEITGAKSPSNLNREAKIQSQ
                     AGEKLFLEFLKKLAEYNEKKQRGGGKKRPERREETKREEYQEDTKRDEYRDDSRKDEA
                     PGDDRRADDTRTDDADRAKPDDSSHLGMVADPNAPAFNWRDAGKMTPVKHQLTCGSCW
                     AFTACAVYEAIYLTANRVSLNLSEQQLVNCAADNRGSKAGTCDGGWYGKVFEYWTRTG
                     AVLTTSVPYRNANGSCTAFPRTTYKVAAWGYVKQDAGIPSVAEMKRALCKYGPIASTV
                     KVTPAFQAFAGGIFDEHISTIGPRDVNHGIVIVGWDDSKKSYLIKNSWGTQWGEKGYM
                     WIEYGCNNIGYGSAWMVPTSEKF"
     gene            3608..4021
                     /locus_tag="PKJ16_03440"
     CDS             3608..4021
                     /locus_tag="PKJ16_03440"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HNV46070.1"
                     /translation="MLQKLQLLLFTALLTTTFVLCAGCEKNKADMKTIADADNGTTVS
                     LAKGESFRIRLKSQMSTGFSWQVTKSPQIVSLQGKPEVEVEKRAEKIVGGVDHQVFTF
                     TAAKEGDGAIDLAYARPWEKKAKPARTFSVKISIK"
     gene            complement(4084..4698)
                     /locus_tag="PKJ16_03445"
     CDS             complement(4084..4698)
                     /locus_tag="PKJ16_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNV46071.1"
                     /translation="MYCHNCGKDVIEGGVYCSFCGSTLDHPRDDAGGRPLYLLKPVFL
                     SIKMLAATVAWQIFLTLWAMVIFGIVGVLMISHFGWDTSHWYFVITAGAVFFVMTPVA
                     YIIIMLNRYTRTEYRFYKDRLEYWEGYFRLRKKQLRYADMIEINLKRGILQRLSQLGD
                     IVISSSATSASDGIAESGIRIFDIYEPDPVFNYLKKNVRSGAAS"
     gene            complement(4781..5947)
                     /locus_tag="PKJ16_03450"
     CDS             complement(4781..5947)
                     /locus_tag="PKJ16_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46072.1"
                     /translation="MGKILSYIPIALALVSFAVFLRPAPSLCAEERLSTVVVEFEEKE
                     NTGIELVSRQVSERLVLYLQASGLYAVAERPLAWTYVRAKRPDLPAPAVRDTAVAIGQ
                     VFGYRVVVIGSITKIDNRVIIIAEAVNVDKRTTVAHAKVEFFETRDMDDRVHDLAGQI
                     IRGERRAGRKTAERPADLMLRHRIGFRLGTGYLFNDTRTDDSKGFAPLAVGAFYNGPF
                     LDVELFGITPPTNAALLSGAINAYPTQVFGVGLGYFWIYDHLELARRDRLLKEYHKGE
                     YQSAVAGVVVRAPFGLKVSFYVGFPVSGTINYQDEDNAWYRYDIRERYQMAPPSLMGF
                     VEYRFNEYISVRASYHQGGGGAEIRSKPEGSDPFPTMHMDTKIITVSVGYGVDL"
     gene            6030..6719
                     /locus_tag="PKJ16_03455"
     CDS             6030..6719
                     /locus_tag="PKJ16_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HNV46073.1"
                     /translation="MISHPWDVTPAEAVRVQRELAESVVIGGMPDPVRTVAGIDIALG
                     HADARGYCGIIAFSYPELVIVDRAFAEGAIGFPYVPGLLSFREGPLILAALAQLAIRP
                     DLLVFDGQGIAHPRRLGIAAHLGLVTGIPSIGCAKSRLYGAYTEPPAERGARTYLVDP
                     SGAPIGVTLRTRTGVRPVFVSPGHRIGVDEAADFMMSCVSSYRVPTPTRVAHIEVGAF
                     KARRLLDAERL"
     gene            6871..7278
                     /locus_tag="PKJ16_03460"
     CDS             6871..7278
                     /locus_tag="PKJ16_03460"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV46074.1"
                     /translation="MDYIQWSDNLSVGVQVFDEEHKNLVGFVNRLNNALTIGSAKKTM
                     EEILSSLIRYTIIHFKHEEDYMALHGYPALEKHRAEHEALKAQVADFHERFVAGKTTF
                     SLELMTFLRDWLTNHILGSDMAYRGFFNERGVQ"
     gene            complement(7293..7796)
                     /locus_tag="PKJ16_03465"
     CDS             complement(7293..7796)
                     /locus_tag="PKJ16_03465"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46075.1"
                     /translation="MDLSAIKRLDVILLCGVYGAGKTEFAQRFLKQPDRSRVSRNEIR
                     KMMFEMTRFGQKWTAADFTEEDDILVKHIERKVLEHFLFNKRRVLVINTFATKRSRQG
                     IVGIAKQMNKTIGAVFLNIPLEKCLLRYKEHNPTLPERVITSMFNKIELPDRREGFTD
                     VLVVTDF"
     gene            complement(7813..9669)
                     /locus_tag="PKJ16_03470"
     CDS             complement(7813..9669)
                     /locus_tag="PKJ16_03470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HNV46076.1"
                     /translation="MEGIRYMMWARCVALVCALWVVAAPASAKRESSERAVSSKIDRV
                     MVYYDRALVTRVARIDAIPAGVSDIIIPNLPPLLQDDSVRARIVEGAEVKILDVEVKM
                     VRLEKAPEEKVRDLQERLQELGDERTRNQNRLKVLEREREYLAKARDSFLGVNRPPRP
                     AGTADAQDPVRLRIKDYDAMLSYLKDKYAANADAVLRAEQKGRAIATSIDLVKGELAK
                     LNVGQSAIQSKKLVRVTVDAHRGGSCALEISYINYKIKWRPAYDIRVALDEKKMDFTG
                     YGIVDQSSGEDWIDAKLSYSTAQPSVRGSLPDLLPLYATDAGKVRASSGKQSRGFASQ
                     SLMNKAVLSGDLGGAREGRASDEPVSESAADASADRSAGSLVFSVPRRSNVPSDGSPH
                     RTVIGRYSFPVKFEYLSVPRLSPYAYLQAVGTNGMKEPVLRGDLNIFMGNDFVGSAYT
                     DTILPGQDFELSLSVNENVRVTRSLEEKEEKKASFITGAQRTTYAFRIKVENYSGGDI
                     VMNVIDQIPVSETQEIEIDEVRFSPEPTVRSKKGILKWQLPVRSRETREISFSFSVNV
                     PKGKEAAFFRTGLSPAQYLQNLSRQNVDYYQENMDQPSSPRKKSPAMRQMLK"
     gene            9971..10852
                     /locus_tag="PKJ16_03475"
     CDS             9971..10852
                     /locus_tag="PKJ16_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46077.1"
                     /translation="MGIIEKIAIASILLLLIAAINSKDSSAGMYFALIMMICFLAFIV
                     YIIAHRGFIRRTGPTDTESTRKELIDSVRDRDGLDRMHYLALLVDRFRNDPHVAKLLA
                     ELLADPDIDMQIEAARHLGARGLAHLLGMLALADTVHDDAALRILGILQEREQGQRGG
                     TDELVRAYERRESEEVREAILGVISHNADPAAGSFLLAELARDGGDLRVPIVEALGQC
                     GSVAAVEPLYRVSSGSLNPFVRSAAAKAIERIQSRLGTAERGWLSPAPLGGADGALSV
                     ADGAGDGALSIPPKKKD"
     gene            complement(10898..12334)
                     /locus_tag="PKJ16_03480"
     CDS             complement(10898..12334)
                     /locus_tag="PKJ16_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46078.1"
                     /translation="MSTIALVEASADSTHYYSSTYLPRMGMPTLGAVLARAGHACDIW
                     FQAMPGFDEERLFEYDIVCISSLSNTIDEAYRLADALTAAGRTVIMGGAHVSFLPDEA
                     LDHCPYVVIGEGEDVLPALVDALARGGPLDEIRGLAYRSPDGRGRASISATAGLVDFE
                     RLPSPDFSLSPQVAPGNTPPIVTTSRGCPHDCSFCSVTSMFGRKYRFKSSAQVIEELR
                     PLKHRSVFFGDDNIYASPRRAKALLRDLIAADALPLRWSGQMTVKAALDEEFLGLLRE
                     TRCRIMYIGIESVDPETLKGYGKAHQADAIAHCIENMHRYGIGVHGMFVVNADDGPDA
                     VKKIVDYAIATDIDTIQICALTPFPGTAAYDEFKGRLLHTEWKYFDGMHVVVDPSACT
                     AYELQMTIVRELKRFYSLTRVLGAYRRGRAWRLKYRAGGYYIARRWVRENREYLSRLR
                     DELCPRTRTWRAGEPIVAPEPVPGGVSR"
     gene            12778..14772
                     /locus_tag="PKJ16_03485"
     CDS             12778..14772
                     /locus_tag="PKJ16_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV46079.1"
                     /translation="MGLAEDIIYIIIAGLATGLVANRLGIPPIVGYIIGGIIIGPHTG
                     GITVSDVPRIELLAEIGVALLLFSIGLDLSFKEIRSVRAVALIGTTVQIALTALFGFG
                     MGRLMGLPAPASVVLGMTLSLSSTMVVLKTLMNRGLMGTLSSRVMVGMLIVQDLAAIP
                     LMIFIPHLVSTGDLASLGLVILKAAVLLGAIVVVGMRVLPLVLKTVARLESRELFLIT
                     VTAIGLGVGFCTHLFGLSLAFGAFVAGMVINQSEYSHQALSDIIPLRDIFGLFFFTSI
                     GMLLDPAFLLANLSTVAMLVPAVIGAKFIIFFAITRAFRYRNIVPLAAGLSLAQIGEF
                     SFVLARTGLQNNVIDRGLYALILATAVITMIVTPFLAMLAGPLYSLRNRFFRTERYQT
                     VNLPAGGLTDHVVIAGGGRVGATIAGILHHLGVACAVIDQDFRRFEQARGIGCTTVFG
                     DATQEPVLAAAGVMRARLLIMTVPSIQSARTIIATALSLAPGIAVIARGDSDEQADEL
                     YAMRVREVVRPEFEAGLEIIRQSLLALDYPAAAIQQYTDDVRRSRSSPLLRANADYSL
                     LAGLRSAAHLLELAWIDIAPESPAAGRSIKDLAVRSRTGVSVVGVFRDGLFTPNPDAD
                     FSLRENDHVAIIGTPAGKKAFESLVAPGEAGNQPHTEAGT"
     gene            14769..15242
                     /locus_tag="PKJ16_03490"
     CDS             14769..15242
                     /locus_tag="PKJ16_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3124 domain-containing protein"
                     /protein_id="HNV46080.1"
                     /translation="MKALLCIVSIGVAALLLTAGAAAPKQRGTAPRPSACYVPVYSAV
                     HYGDRQHAMNLAVTLAIHNTDLRNAIMITAVDFYDTAGRKLKSYLATPRALNPLETVQ
                     YYVRESDTSGGTGANFIVRWRAASGAGAPLVESIMIGTHGQQGISFTSRCVPIPD"
     gene            15278..17560
                     /locus_tag="PKJ16_03495"
     CDS             15278..17560
                     /locus_tag="PKJ16_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46081.1"
                     /translation="MSDMLNGKTILLVEDEAIIAMDEAALLERRGCAVIVASSGSAAV
                     DAVLRNPHIDLVLMDIDLGAGVDGTAAAERILAHREVPIVFLSSHTEPEVVERTEGIT
                     SYGYVVKDSGETVLLASIKMAFRLFAARTALTEKSDEAEAANEELGATIEELESTNEE
                     LARSQEELLQLTTLLEQVFEQSPVPMALVSMPDVTIRIANRACREFLGILDEPSPVGT
                     SLPDFRPTFEDRDMSGALGAVGDLPLARSLRGERTVNEERIIVRKDGTTRRELVNAVP
                     IVNERGEIIAGYLTLIDITERRKIEMMFEAIFRLSPIAIALTRFEDGRIVQANDRYCA
                     MTGYRLDEIIGKTSLELNFWPHPGDRDTVKRILAKNGSFRDREITFRRKDGSLYDSQQ
                     SASVITIDGERYIIAATKDLSDERAMRETLQHREALITAISDNLPGAMIYQIDRRPDG
                     TRVITHLSDTVRRFYGCSPEEAIADPTRIYGRVADEDTARLFEAEEEANRSLSRLDIE
                     VRMIDPSGAPRWSHFVSTPALLPDGTTRWDGIEVDITERKRIEDELARSLAAQETLLR
                     ELRHRVMNSLSIIGSLAGMEGRATESAAVRAALLSMGGRIRSLAELYDMLPLSAAGEI
                     RLDLYLRRIIDAFARSILHDSSRIVITATGDEISIDADRATTLGLIANELATNAVKYA
                     FPDGRAGAVNATLRRDGIDIVLMMSDTGIGPPPGFSLSGGTGTGMSIVTMLARQLDGS
                     VSFERIDGRTVFTVRVPYGA"
     gene            complement(17581..18111)
                     /locus_tag="PKJ16_03500"
     CDS             complement(17581..18111)
                     /locus_tag="PKJ16_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV46082.1"
                     /translation="MIDFITDREIYERVILGAAPSAERFLWIATADLKDLYVSQGTRM
                     VPFLAVLSGLIDRGVSVRLLHAKEVGPHFRDDFDRHPNLIDGLERIQCPRVHFKAVIV
                     DGRFAYTGSANLTGAGMGAKSDARRNFEAGVVTDERALVKRIMAQFDEVWMGARCVEC
                     QRKEYCTDYPDLLAGR"
     gene            18251..18808
                     /locus_tag="PKJ16_03505"
     CDS             18251..18808
                     /locus_tag="PKJ16_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46083.1"
                     /translation="MADQPYLFAVLVVGAAIAYVVYGLVRNKSPREAWEALAGVRGPG
                     AALAECAVTPGAPFSLTHRASRASVLRAFLACEIAYRLDGADGIDDERAFGIAVRIDM
                     GEPTGPPRSVRLGIGRLAPPDVIAFTGVMHGTRFSRTGGECRFRAVVPIGRMPVAAGA
                     EAVIAGTVEASANITATAITVLITT"
     gene            18858..19463
                     /locus_tag="PKJ16_03510"
     CDS             18858..19463
                     /locus_tag="PKJ16_03510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV46084.1"
                     /translation="MIRITERADIRAAASVLAEAFRDDPLFAHFFPDASRRRDRAVHT
                     FRFIVSHAVGRGRVYLSPRRDAAAVWLPPGISERGLADQIRYGALRMLVRQHPRSIVR
                     QMRASDFMHAIHERFLPAHRWYLSTLGALPSARGTGAASALVRGMLDEIDTGALPCAL
                     DTHNGNNISFYARFGFTVVHESAIPGTSVRHWTLLRPTRAR"
     gene            19468..19956
                     /locus_tag="PKJ16_03515"
     CDS             19468..19956
                     /locus_tag="PKJ16_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020887418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HNV46085.1"
                     /translation="MPSAPAPSITIISGGQTGVDRAALDAAIALGVPHGGWCPAGRLA
                     EDGTLPARYLLTETRSRDYSVRTERNVRDSDATLILTWGPPSGGTAYTIECAIRMNRP
                     YLVVDMEGESADPARAANWMRDRDIGALNVAGPRESSRPGVVHSTAMRFLVALLEALL
                     HQ"
     gene            complement(20013..21293)
                     /locus_tag="PKJ16_03520"
     CDS             complement(20013..21293)
                     /locus_tag="PKJ16_03520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HNV46086.1"
                     /translation="MEHENCSDTVAKCESPTLMERIAMRMVLSHLTAIRHGRLLVVLP
                     DGESRSYGSHGDSHRAVLQVRRYRFFTRVLFGGEIGFGEAFTAGLWDSPDVVGVINCF
                     IDNTDALGDAARSANRLSRVAQYLVHRLHANSIFRSRKNISAHYDLGNGFFSSFLDRS
                     MMYSCAIFRSKKDGLERAQRNKIHAIIDKAGITAGDHVLEIGCGWGGFAIEAARTTGC
                     RVTCITISGEQLAMARERIAAAGLSDRVRVEFCDYRAVRGSFDKIVSIEMLEAVGHRY
                     LGDFFAALERCLAPSGIAVVQVITTPDHHYRAYCRRADWIQKYVFPGGHLPSLGALVN
                     AMLKRSTLFVEHVENIGPHYATTLRAWRERFDAVAERLEQTGYDQMFQRLWRYYLSIC
                     EAGFESRIVNDLQIVLTRPSNRTVPRYVTSFENG"
     gene            21706..21900
                     /locus_tag="PKJ16_03525"
     CDS             21706..21900
                     /locus_tag="PKJ16_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HNV46087.1"
                     /translation="MACDRMEKNLAMCNCSYEPCSRKGKCCECIQYHRRSSELPACFF
                     PDSVERTYDRSVRRFIQCQK"
     gene            21888..22529
                     /locus_tag="PKJ16_03530"
     CDS             21888..22529
                     /locus_tag="PKJ16_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46088.1"
                     /translation="MPEVMPITRTAARIMLFAACFALGCASFRDSRFVPPFDFTPPAR
                     DAGALLIINRFAGPDTTAGSIVSEADRESFVATVEGACRDARLFSRTVTQRRAGRERD
                     AGYAVILETELAISENLHPKSARVLNTFLFTWLDRRRVTLRASVYDGGGTRRGEYEMS
                     ERFETIGFLLLPVFPLLGRSERTVKDEVFRTLVVRVINAAQRDGAFAAPKPKE"
     gene            complement(22686..23102)
                     /locus_tag="PKJ16_03535"
     CDS             complement(22686..23102)
                     /locus_tag="PKJ16_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV46089.1"
                     /translation="MVVFLDTSSLLKRYVEERGSDQVDRFFVSGNAICISPITPIEMY
                     SALCRKVADRSIDQETFDKAQRYWGQDIRHYSIVPFSEILVERAISIIEAHNIRTLDS
                     IQIASASLSDPDTCITSDIAMSKVLEKVLVGRTLVV"
     gene            complement(23096..23299)
                     /locus_tag="PKJ16_03540"
     CDS             complement(23096..23299)
                     /locus_tag="PKJ16_03540"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HNV46090.1"
                     /translation="MIKLEDIQRMPDEIKKEILDYAEYLIAKYQSERDGEEVRWVDVR
                     SRGRSIGEMASDTVVKLRNEARW"
     gene            23586..23825
                     /locus_tag="PKJ16_03545"
     CDS             23586..23825
                     /locus_tag="PKJ16_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46091.1"
                     /translation="MINITIDEKRELLEGIERQYRNMAPDEKDELLTVAIMLKLYEKN
                     NGFPARNMNDLEMWYSLNKAAVDMLPDPEEGDAGV"
     gene            23894..24175
                     /locus_tag="PKJ16_03550"
     CDS             23894..24175
                     /locus_tag="PKJ16_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46092.1"
                     /translation="MTISRRVSYTGLALSGLYVFIATVALVLSSDCRGEGCAVQLFVT
                     FPWFFLFLRSSSTIMLFVVSVSLNAILWYLLGSAIGRLGKWIVSRLGRG"
     gene            complement(24241..26067)
                     /locus_tag="PKJ16_03555"
     CDS             complement(24241..26067)
                     /locus_tag="PKJ16_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46093.1"
                     /translation="MILCAIAALASFSCSMFGGRLFTAIEHGDSKAAIELIRTHPSIT
                     SSRQDHKNNPLTPLMYAAVYNRSDVAACLIESGVDVNETTGEYTFRSGWTALHYAAAY
                     NAPETAIVLIKAGAKPTGRAPGQGWTPLHVAASYGHVGPLKAMLDAGTPPDARDGRGT
                     TALMAASRSMNISDDTALVLLKYGADPNVRGGQIDPIRTNWTALMWAAFYNKTDLVRA
                     LIARGASPDIKSGEGMTALMVAADVGIHSAQRIPVVRALLESGASTSVRDNEGKTALH
                     HNIKNLYLESYDIAVLKLLLQHKADVNARTNDGETPLVFAVKQASDASKVRHKIEVIR
                     TLLAVGADVNIATKNGRTALYELLDRPDLGGKLGKGAYDPAAMLDMVLAAGADVNARC
                     GSEQRTALFPAVYWNYADAVRALVRHKAEVNVQDAGGYTPLILAVRGNALTSIGILLS
                     AGADIAMENKHGRSAIGEAAAADRDRVIALFVERGISLAVREKSTGWTPLMIACASGS
                     NAAAKAIVNARVNLNETDRRGRTALMIVADARAREIDLLKMFDTARGAAGPADYAAIA
                     ELLIGAGADVNARDLSGESALSYARKTRQASVEQVLLKHGAK"
     gene            complement(26170..26898)
                     /locus_tag="PKJ16_03560"
     CDS             complement(26170..26898)
                     /locus_tag="PKJ16_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV46094.1"
                     /translation="MFTLIHTCDDFIVISKKAGVSFHGGDGAPSLDDAIRERMGIDEL
                     YALHRLDNLTSGLLVFARTRGVVRELARQFRERHIEKFYLAVGGPHPKKKQGIVSGDM
                     KKGRDGVWILTRTHERPAVTRFFSTFLQPGLRLYLLRLYTGRTHQVRVALKSVGAPVI
                     GDARYCKKVMAGIEPDRGYLHAYALGFSVMGVRHRFVCPPDEGRYFLSDEFKAALIRL
                     GEDPWALTWPKTQVHGLSPEQSGI"
BASE COUNT         5097 a         8729 c         8227 g         4905 t
ORIGIN      
        1 aagctcatcc ttgccggcga gaccaacacg ttggaccgtc tgctggatga cctcgtcgcc
       61 tttagcggca ccgaccagtt cgaggacgac gtgacgatga ttttgttcga gttctgacgc
      121 gccgcttgac gcatcctcgt tttttccgta tcttcatgtg tgaagctggc gctcattaca
      181 tccttctgca cggtgaaacg atgaaacggg ttctctcggc cataatcctg tcagtggtgc
      241 tgattgcggc aggctgcaat caacaagacc tgttcgattt gacatcgaaa gcacaggatt
      301 tgaaaacaag cgggcgtctt ccctccgacg tcgccggtct tgcgctgtgg ctgaaggctg
      361 acgggataac tcccctgccc gatgggaccg ttataagcga ctggcctgat gcttcgggga
      421 atggaatcac cacagtggtt ttttcaaacc cgcgatattt ccataccgcc tttaacggaa
      481 tgcccgcagt gcgatttgac ggtagttcat atattctcgc ttccgagttc atgaactttt
      541 cgtcttttac cattttctat gtcggtgcgt acactggacc gggaggcgga cggcatatca
      601 tctatgacca aggggtgacg gcgggttcga caccgtttat cggattgcac attagcacgg
      661 tttcaattac cggaaccgtt ggcgactcac aggcaacccc ggagtttcta tccatcacaa
      721 gtcccttaac gcccacaacg ccccatatcg ctgtttttgt gctaaatgga cagaacgcct
      781 cgctatttct taacggcgct tcatccggtg gaacgttgaa cttggcctac gactcatcga
      841 caacatggtt caatggcccg agcgctccga gtattgggag ccaaaacggc gtcatgtatt
      901 atcagggcga catcgcagag ataatcgtgt ataacaacgc cctcagcgaa accgacgggt
      961 tcgccgtcga ctgctacctc agcaagaagt acggcatcgc cgtatcgcac ccctgcgact
     1021 aggcgctacg cgcgatagta ttcccgatac cacgccacga aacgcgcaag cccctcggca
     1081 aagggcacgc gcgggcaata gccgaactcg cgcgcgaggt cggcgatgtc cgcgcgggtc
     1141 gcgagcatat cgcccggctg gcggggcgtc gcgatgacgg tcgctgttgc gcccagcgcg
     1201 tgctctatgg cgcgcacgaa ctcctcgatt gacacggacg ccccgcttcc cacgttgtag
     1261 atacgatacg gcgcggccga caaggccgga tcggcaccgt cccacgcccc gtccggcagg
     1321 ggcggtcgct caagcacggc aacaaccgca tcgaccacat cgtcgatgta cacgaaatcg
     1381 cgccgcaggc ccccgtcccc gaagagctcg atcgcatcgc cgtcgatgat gcgcttcgtg
     1441 aacgaaaaat acgccatgtc cggccgcccc caagggccgt agacggaaaa gaagcgcagg
     1501 cccgtcgtcg ggatgccgta gagatggctg taggcgtgcg ccatgagctc gttcgcgcgc
     1561 ttggtcgcgg cgtagaggct gatggggtga tcggcgggac cgtgcggcga gtacggggcg
     1621 cgccccccga gcccgtacac cgacgagctg ctggcgaaca cgagatggcg cacgggcgcc
     1681 gcgcggcatg cctcgagcat aacggcaaac ccgttgatat tgctctcgac gcacgatgcg
     1741 ggccggatca gcgaatggcg cacgcccggc tgcgcggcca gatggcacac cgcgtcgaac
     1801 cgctcgtcct cgaagagcgt tctcagacgg tcgccatcgc acagatcgag ccagatgaat
     1861 cggtgccccg gcagcgcgct gcttctcacg agtgcgccat acgaaatcgc gttgtcatcg
     1921 atcccggacg ctcgcagtcg cgccatcttg agcgccggat cgtagtagtc gtttatgcta
     1981 tcgagcccca cgacctcgca tcccaacgcc gaaagccgcc gggcgacatg atgcccgata
     2041 aacccggccg aaccggtgag tagcaccttc atcgcttttt tcgccccatg ctagaagtat
     2101 cggccagcat gcgaaggggg cttcgtattg ttttaccggg cgattgcatt attgacacta
     2161 gttgatattt gcgcatataa gaaatatcac ccctctttta aaaaaagaat ttgaatttta
     2221 cccgatgcct gcttatcaat aaaaataatt gcacatgccc gaacgaccgt cgtctagtat
     2281 ggaacggaca tcgatggcgc ttcgacgcca cagtgcgggc aataacggac acagagaggt
     2341 caccatgaat agagccctca ggatcctatg cgtgagcgcg ctcgctgttg cgttcatcac
     2401 gggcgatacg aacgtttcgt tttcgcagta cggcaagaag tacaaggagg cggtgcagcg
     2461 ccagcgcgag gtacaacaga aggacctcgc cgaggtgcgc cagcaggtcg aggccatccg
     2521 gcagcaaatc aaggagaacc actggaagtt caaggccgag atgaccgagg cgctcaaata
     2581 ccgcatcgag gagatcacgg gcgcgaaatc gcccagcaat ctgaaccgcg aggcgaaaat
     2641 ccaaagccag gccggagaga aactcttcct cgaattttta aagaagctcg ccgagtacaa
     2701 cgaaaagaaa cagcggggtg gcgggaaaaa gcgccccgaa cggcgcgagg aaaccaagcg
     2761 cgaggagtac caggaagaca ccaagcgcga tgagtatcgc gacgattccc gaaaggacga
     2821 ggcgcccgga gacgaccgtc gggccgacga tacccgcacc gacgatgcgg accgcgcgaa
     2881 gccggacgat tcgtcgcacc tgggtatggt ggccgatccc aatgcgcccg ccttcaactg
     2941 gcgcgacgcc ggcaagatga cgcccgtgaa gcaccagctc acctgcggga gctgctgggc
     3001 cttcaccgcc tgcgccgtgt acgaggcgat ctatctcacc gcaaaccgcg tatcgctcaa
     3061 tctctctgag cagcagctgg tcaactgcgc agcggacaat cgcggcagca aggccgggac
     3121 ctgcgacggc ggctggtacg gcaaggtgtt cgagtactgg acgaggaccg gcgccgtcct
     3181 cacgacaagc gtaccatacc ggaacgccaa cggctcctgc accgccttcc cgcgcacgac
     3241 ctacaaggtg gccgcctggg ggtacgtaaa gcaggacgcg gggatcccct ccgtggccga
     3301 aatgaagcgc gccctgtgta agtacggccc catcgcatcg accgtgaagg tcaccccggc
     3361 cttccaggcc tttgccggcg gcatcttcga cgagcacatc tcgaccatcg gcccgcgcga
     3421 cgtcaatcac gggatcgtga tcgtgggttg ggacgacagc aagaaatcgt atctcatcaa
     3481 gaactcctgg ggcacgcagt ggggcgaaaa gggatacatg tggatcgagt acgggtgcaa
     3541 caatatcggc tacggatcgg cctggatggt cccgacgagc gaaaagttct aactatggag
     3601 agttgtcatg ctacagaaac tgcagttatt gctgttcacc gcgctgctta cgaccacctt
     3661 cgtgttgtgc gcgggctgtg agaaaaacaa ggctgacatg aagaccatcg ccgacgccga
     3721 caacggaacc acggtgtccc tggcgaaggg cgagtcgttc cgcatccggc tgaagtcgca
     3781 gatgagcacc ggctttagct ggcaggtcac gaagtcgccg cagatcgttt cgcttcaggg
     3841 aaaaccggag gtcgaggtgg aaaaacgcgc cgagaagatc gtgggcggcg tcgatcacca
     3901 ggtgttcacc ttcacggccg cgaaggaggg ggacggcgcg atcgacctgg cctatgcccg
     3961 cccgtgggaa aagaaggcga agccggcgag gaccttcagc gtaaagattt ccatcaagta
     4021 acgatcatac gcgattgtcc gcgcacacgt agaggcgggt caccgacccg cctctatccg
     4081 ttattacgag gccgcgccgc tgcgcacgtt tttcttgaga taattgaaca ccgggtccgg
     4141 ctcgtagatg tcgaaaatgc ggatgccgct ttccgcgatc ccgtccgacg cgctggtcgc
     4201 cgacgaggat atcacgatgt cccccagctg cgagaggcgc tgcaggatcc cgcgcttcag
     4261 gttgatctcg atcatgtcag cgtaccgaag ctgttttttc cgaagccgga aatacccctc
     4321 ccaatactcc aggcggtcct tgtaaaaccg gtactccgtg cgcgtgtagc ggttgagcat
     4381 gatgatgatg taggccaccg gggtcatgac gaagaagacc gcgccggcgg tgatcacgaa
     4441 ataccagtgc gaggtgtccc agccgaaatg gcttatcatg agaacgccga cgatgccgaa
     4501 gatgaccatg gcccacagcg tgaggaagat ctgccaggcg accgtcgcgg cgagcatctt
     4561 gatgctcagg aacaccggct tgagcaggta gagcggccgc ccgccggcat cgtcccgggg
     4621 atggtcgagc gtggagccgc agaacgaaca gtatacgcca ccctcgatga cgtccttccc
     4681 gcaattatgg caatacatcg gcaccctcct gcccgtccgg catatatggg actctttata
     4741 tatatatcgt acaggtctgt caatatttta acgatttccg ctacagatcg acgccatacc
     4801 cgacggaaac ggtgatgatc ttggtgtcca tatgcattgt cgggaaggga tcgctcccct
     4861 ccggcttcga tctgatctcg gctccgccgc cgccctgatg atacgatgcg cggaccgaga
     4921 tatactcgtt gaaccggtac tcgacgaagc ccatgagcga cggcggcgcc atctgatagc
     4981 gctcgcggat gtcgtaccga taccacgcgt tgtcctcgtc ctgataattg atggtgcccg
     5041 acaccggaaa cccgacgtag aaggacacct tgagcccgaa gggcgcgcgc accacgacgc
     5101 ccgcgaccgc cgactggtat tcgcccttgt gatactcctt gagcagccgg tcacgacggg
     5161 ccagctcgag atgatcgtat atccagaaat agcccagccc caccccaaag acctgcgtgg
     5221 gataggcgtt gatggcgccg gacaagagcg ccgcgttcgt ggggggcgtg atgccgaaga
     5281 gctcgacgtc gagaaacggc ccgttataaa aggcgcccac ggcgagcggc gcaaatccct
     5341 tgctgtcgtc tgtccgggtg tcattgaaca ggtagcccgt ccccagccgg aacccgatcc
     5401 gatgccgcag catgaggtcc gccgggcgtt cggcggtttt ccggcccgcg cgccgctcgc
     5461 cgcgtatgat ctgcccggcg agatcgtgga cacgatcgtc catatcgcgc gtctcgaaaa
     5521 actcgacctt ggcgtgggcc acggtggtcc gcttgtccac gttcaccgcc tcggcgatga
     5581 tgatcacgcg attgtcgatc ttcgtgatcg agccgatgac cacgacccga tacccgaaca
     5641 cctgcccgat ggcgaccgcc gtatcgcgca cggccggcgc gggcaggtcc ggccgcttcg
     5701 cccggacata ggtccacgcc agcggccgct cggccaccgc atacagcccc gacgcctgca
     5761 aatacaggac gagccgctcg ctcacctggc gcgagaccag ctcgatcccg gtgttctcct
     5821 tctcctcgaa ctcgacgacg accgtcgaca ggcgctcctc ggcgcagagg gacggcgccg
     5881 ggcgcaggaa tactgcgaat gaaacgagcg caagcgcgat cgggatatat gatagaatct
     5941 ttcccatgtg ttttccgccg attgtacttg cccaatttgg gccaatggcg taaaaaaagc
     6001 atcaacattt ttccaaactc gaggacgcca tgatatcgca cccctgggac gtcacgccgg
     6061 ccgaggccgt ccgggtccag cgcgagcttg ccgaaagcgt cgtcatcggc ggaatgcccg
     6121 atccggtccg caccgtcgcc ggaatcgaca tcgcgctcgg acacgccgat gcgcgcgggt
     6181 attgcggcat catcgccttc tcgtatcccg agctcgtcat tgtggaccgc gcattcgcgg
     6241 agggggccat cggctttccg tacgtgcccg gcctcctctc gtttcgcgag ggaccgctca
     6301 tccttgcggc ccttgcgcag cttgccatcc ggcccgacct cctggtgttc gacggtcagg
     6361 ggatcgccca tccgcgtcgg ttgggcatcg ccgcgcacct gggactggtg acgggcatcc
     6421 cgtctatcgg gtgcgcgaaa tcgcgcctgt acggcgccta caccgaaccc ccggccgagc
     6481 gtggcgcgcg aacctacctt gtcgatccct ccggcgcgcc catcggcgtg acgcttcgaa
     6541 cgcggacggg cgtgcggccc gtgttcgtgt cgccgggcca ccgcatcggc gtggacgagg
     6601 cggccgattt catgatgagc tgcgtatcgt cgtatcgcgt gcccacgcca acgcgggtgg
     6661 cgcacatcga ggtgggcgca ttcaaagcgc ggcggcttct tgatgccgag aggttgtagc
     6721 gagttcgatc ctcaagtcga catcatcgcg attgacctat cgccaccgtc gcgcgccgta
     6781 aaaaaaacgc cccatcgcac cgataaaaaa ttattgccgc aggacggcat ccgtgctacg
     6841 tatttacata caccactcgc ggagaacgcc atggactaca tacagtggag cgacaacttg
     6901 agcgtcggcg tgcaggtttt cgacgaagag cacaaaaacc tggtcggctt cgtcaatcgg
     6961 ctcaacaacg ccctcaccat cgggagcgcg aagaagacga tggaggagat cctctcaagc
     7021 ctcatccggt acaccatcat acatttcaag cacgaagagg actacatggc gctccacggc
     7081 taccccgccc tcgaaaaaca ccgcgcggag cacgaggcgc tcaaggccca ggtggccgat
     7141 ttccacgagc ggttcgtcgc ggggaagacg accttctcgc tcgagctcat gaccttcctt
     7201 cgcgactggc tcaccaatca catcctggga agcgatatgg cctatcgggg gttcttcaac
     7261 gagcgcgggg tccagtaggc gctcacccgc ggctagaagt ccgttaccac cagcacgtcg
     7321 gtaaaaccct cgcgacggtc cgggagctcg atcttgttga acatggacgt gatcacgcgc
     7381 tcggggagcg tggggttgtg ctccttgtag cgcagcaggc atttctcaag ggggatattg
     7441 agaaacaccg ccccgatggt cttgttcatc tgcttcgcga tccccacgat gccctggcgc
     7501 gaacgcttgg tggcgaaggt gttgatgacc agcacccggc gcttgttgaa caggaagtgc
     7561 tccagcacct tccgctcgat gtgtttcacc agaatgtcgt cctcctcggt gaaatccgcg
     7621 gccgtccatt tctgcccgaa gcgggtcatc tcgaacatca tcttgcgaat ttcgttgcgg
     7681 ctcacccggc tgcggtcggg ctgcttgaga aaccgttgcg cgaattccgt ttttccggcg
     7741 ccgtacacgc cgcacagcaa gatcacatcc agacgtttta tggcgctcag gtccatgtgc
     7801 ggacacctcc gtttatttca gcatctggcg catcgccggg gatttcttcc gcggcgacga
     7861 cggctgatcc atgttctcct ggtagtagtc gacgttctgc cgcgagaggt tctgcagata
     7921 ctgcgcgggc gagagcccgg tgcggaaaaa ggccgcctct ttgcccttgg ggacgttcac
     7981 cgagaacgaa aaggatattt cacgcgtctc gcgcgaccgc accggcagtt gccacttgag
     8041 gatgcccttt ttgctgcgga ccgtcggctc cggcgagaag cggacctcgt cgatctcgat
     8101 ctcctgcgtt tcgctcaccg ggatctggtc gatcacgttc atcacgatat cgccgccgct
     8161 gtagttttcg accttgatcc ggaaggcgta ggtggtcctc tgcgcgccgg tgatgaagct
     8221 cgccttcttc tcctccttct cctcgaggga gcgggtcacg cggacattct cgttcacgct
     8281 cagcgagagc tcgaagtcct gcccgggaag gatggtgtcc gtgtacgccg agcccacgaa
     8341 gtcgttgccc atgaagatgt tgagatcgcc tcgcaggacg ggctccttca tcccattggt
     8401 ccccaccgcc tgcagatagg cgtagggaga gagacgcggc acgctcaagt actcgaattt
     8461 caccgggaag gaatagcggc cgatcaccgt gcggtgcggc gatccgtcgc tcgggacgtt
     8521 cgagcggcgg ggaaccgaaa acacgagcga ccccgcgctc cggtcggcgg aggcgtcggc
     8581 ggccgattcg gagaccggct cgtcggaggc ccgcccctcc cgcgcgccgc ccaggtcgcc
     8641 ggagaggacc gccttgttca tcaggctctg gctcgcaaaa ccccgcgact gcttcccgct
     8701 ggacgcgcgc accttgcccg catcggtcgc atagagcggc aggaggtccg gcaggctgcc
     8761 gcgcaccgac ggctgcgcgg tggaatatga taatttcgcg tcgatccagt cctcgccgct
     8821 cgactggtcg acgatgccgt atccggtaaa atccatcttc ttttcgtcca acgccacgcg
     8881 gatatcgtag gccggacgcc acttgatctt gtaattgatg tacgatatct cgagcgcgca
     8941 cgaaccgccg cgatgcgcgt ccacggtcac ccgcaccagc ttcttgctct gtatggcgga
     9001 ttgccccacg ttgagtttcg cgagctcccc cttcacgagg tcgatgctcg tggcgatcgc
     9061 gcgccccttc tgctccgctc gcagaaccgc gtcggcgttc gccgcatact tgtccttcag
     9121 gtacgacagc atcgcatcat agtccttgat ccgcagccgc accgggtcct gcgcgtccgc
     9181 ggtcccggcc gggcgcggcg ggcggttcac gcccagaaag ctgtcgcgcg ccttggcgag
     9241 atattcccgc tcgcgctcga gcaccttgag gcggttctgg ttccgcgtcc gctcgtcgcc
     9301 aagctcctgg agacgctcct gcaggtcgcg caccttctcc tcgggcgcct tctccagacg
     9361 gaccatcttc acctcgacgt cgaggatctt cacctcggcc ccctcgacga tccgggcgcg
     9421 cacggaatcg tcctgcaaca gcggcggcag attggggatg atgatgtcgg acacccccgc
     9481 ggggatcgcg tcgatccgcg cgacgcgggt caccagcgcg cggtcgtaat agaccatcac
     9541 ccggtcgatc ttcgacgata cggcgcgctc cgaggattcg cgcttcgccg acgccggcgc
     9601 cgcgaccacc cacaacgcgc acacgagcgc gacgcaccgc gcccacatca tatatcgaat
     9661 gccctccata gcgaccctcc cctgcgcgca gcgatactta tgcgatcgca atcatcatgg
     9721 taagcataca caatcgcgga gaaaaaaaca agcggaattc tcgtgctgcc gcggcggaat
     9781 gcatgcgatc tcggcgaaca tgaaaaaatc cttgatcggg atgcggatcc atgcgtacac
     9841 ataccgcatg cgcgtgtgaa tcattcgagt tgatgaaata tcgtcgttac tgccgtgcac
     9901 gcactatcat cgagcgtcac agcacgtccg gcgcccatcg catcgagcga gggtgacatg
     9961 ggaggctccg atgggaatca ttgaaaagat cgcgattgcg agcattctcc tactactgat
    10021 tgcggcgatc aacagcaagg actcgtcggc gggaatgtac ttcgccctga tcatgatgat
    10081 atgctttctc gcattcatcg tctacatcat cgcccaccgt ggttttattc gtcgaacagg
    10141 ccccaccgac acggagtcga cacgaaaaga actcatcgat tcggtcaggg accgcgacgg
    10201 cctcgaccgc atgcactacc tcgccctgct tgtggaccga tttcgaaacg atccgcacgt
    10261 cgcgaagctg ctcgccgagc tcctcgcgga cccggacatc gacatgcaga tcgaagccgc
    10321 gcggcatctg ggcgctcggg gactcgcgca cctgctgggc atgctggcgt tggcggacac
    10381 cgttcacgac gacgcggccc tgcgcatcct gggaatcctt caagaacggg aacagggcca
    10441 gcggggcggg accgacgagc tcgtgcgggc gtacgaacgc cgcgagtccg aggaggtccg
    10501 ggaggcgatc ctcggagtaa tttcgcataa cgccgatccg gcggccgggt cgttcctgct
    10561 cgcggagctc gcgcgcgacg gcggcgacct gcgcgtgccc atcgtcgagg cgctggggca
    10621 gtgcggaagc gtcgcggccg tggagccgct ctaccgggtg tcgtcgggtt cgctcaatcc
    10681 gtttgtccgg tcggcggcgg cgaaggcgat cgagcggata cagtcgcgcc tggggaccgc
    10741 ggagcgcgga tggctctccc cggcgccgct gggcggcgcg gacggggccc tgagcgttgc
    10801 cgatggagcg ggcgacggcg ccctgagcat tccgcccaag aaaaaggact gaccgccgcc
    10861 aggcggatcg cgcgggagta ttcgcgtcag ccggtcatca gcgcgatacg ccgccgggta
    10921 cgggctccgg cgcgacgatc ggttcgcccg cacgccatgt ccgggtgcgc gggcacaatt
    10981 cgtcgcggag ccgggagaga tattcccggt tttcgcgcac ccagcgccgg gcgatgtaat
    11041 agccgcccgc gcggtacttt aaccgccagg cgcgtccccg tcgatacgcg cccaatacgc
    11101 gcgtgaggct gtagaagcgc ttgagctcgc gcacgatggt catctgcaat tcataggccg
    11161 tgcaggcgga ggggtcgacc accacgtgca tcccgtcgaa gtatttccat tccgtgtgca
    11221 gcaagcggcc cttgaattca tcgtaggccg cggtccccgg gaagggcgtg agcgcgcaaa
    11281 tctgtatggt gtcgatgtcg gtcgcgatgg cgtaatcgac gatctttttc accgcgtccg
    11341 ggccgtcgtc ggcgttcacg acgaacatgc cgtgcacgcc gatgccgtag cggtgcatgt
    11401 tttcgatgca gtgcgcgatg gcgtcggcct ggtgcgcctt cccgtagccc ttcagggtct
    11461 ccgggtcgac cgattcgatg ccgatgtaca tgatccggca gcgcgtctcc cgcaggaggc
    11521 cgaggaactc ctcgtcgagc gccgccttca ccgtcatctg ccccgaccag cgcagcggca
    11581 gcgcgtccgc ggcgatcaga tcgcgaagga gcgccttggc gcggcgcggg ctggcgtaga
    11641 tattgtcgtc gccgaagaag acgctacggt gcttcagggg ccgcagctcc tcgatcacct
    11701 gcgccgacga tttaaagcga tatttccggc cgaacatgct ggtcacgctg caaaagctgc
    11761 agtcgtgcgg gcagccgcgg gaggtggtca cgatgggcgg cgtgttgccc ggcgcgacct
    11821 gcggcgagag cgagaaatcc ggcgacggca ggcgctcgaa gtcgaccagg ccggcggtgg
    11881 ccgaaatgct cgcgcgtccg cgtccgtccg ggctgcggta ggccagtcct cgtatttcat
    11941 ccaacggtcc tccccgcgcg agggcgtcga cgagcgccgg caggacgtcc tcgccctccc
    12001 cgatgacgac ataggggcaa tggtccagcg cctcatcggg aaggaacgac acgtgggcgc
    12061 ctcccatgat cacggtgcgg ccggccgccg tgagcgcgtc ggccaggcga taggcctcgt
    12121 cgatggtatt ggacagcgag ctgatgcaca cgatatcgta ctcgaacagc cgctcctcgt
    12181 cgaagcccgg catggcctga aaccagatgt cgcacgcatg gcccgcgcgc gcgagcaccg
    12241 cgcccagcgt gggcataccc atgcggggaa gataggtact gctgtagtaa tgggtcgagt
    12301 ccgcgctggc ttcgaccagg gctatggtgg acatggcgta cactcctcga gaatacagtt
    12361 ttgttccgac gacacaaagg acgactatga cggtgggacc gatgcggcga ggccgcgtga
    12421 tcgcggcatc ccgtaacgat acgtgcggtt ggcgataccg gcgcatcatc gcatgatgca
    12481 agaggccatg ttccggttct gcaatagcac ccacaatcga taaccgattg cgcgatcaac
    12541 gtacataaag cacgctattt aaacaaacct ttatttcgcg aaccggcctt tttctttcct
    12601 ccgatagcga cagcattgtc cgccaaacgg ttgcgatttt tcggcgaaaa tgccgtttcg
    12661 atgaaacaga attgtacttg ccgcaagaac taccatcgtc attaatcgat ccgtgggaac
    12721 ggtcgttttc tccgggatac gccccagcgg ccttcaccgt ggaccacatg atcgattatg
    12781 ggactcgcgg aagacatcat ctacatcatc atcgccgggt tggcgacggg gctggtcgcg
    12841 aaccgcctgg ggatccctcc catcgtgggc tacatcatcg gcgggatcat catcgggccg
    12901 cacaccggcg gcatcaccgt atccgacgtg ccgcgcatcg agctcctggc ggagatcggc
    12961 gtggcgctgc tgctgttctc catcggcctg gacctgtcct ttaaggaaat ccgctccgtg
    13021 cgcgccgtcg cgctcatcgg cacgacggtc cagatcgcgc tcaccgcgct gttcggattc
    13081 ggcatggggc gcctcatggg actgcccgcg ccggcgtccg tcgtcctggg gatgacgctg
    13141 tcgctatcga gcacgatggt ggtgctcaaa acgctcatga accgcgggct catggggacg
    13201 ctttccagcc gggtgatggt ggggatgctc atcgtgcagg acctggcggc catcccgctc
    13261 atgatcttca tcccgcacct cgtgtccacg ggcgacctcg cgtccctggg gttggtgatc
    13321 ctcaaggcgg ccgtcctgct gggggcgatc gtcgtggtgg gcatgcgcgt gctgccgctg
    13381 gtgctcaaga cggtcgcgcg gcttgagtcg cgggagctct tcctcatcac ggtcacggcc
    13441 atcgggctgg gcgtgggatt ttgcacgcac ctcttcgggc tgtcgctggc cttcggggcg
    13501 ttcgtggcgg gcatggtgat aaaccagtcg gagtacagcc atcaggccct gagcgacatc
    13561 atccccctgc gggacatctt cggcctgttc ttcttcacct ccatcggcat gctgctggac
    13621 ccggcgtttc tgctggccaa cctctcgacg gtggcgatgc tcgtacccgc ggtcatcggc
    13681 gcgaagttca tcatattctt cgcgatcacg cgggccttcc ggtaccggaa catcgtcccg
    13741 ctcgcggcgg gtctctccct ggcgcagatc ggtgagttct cgttcgtact cgcgcgcacg
    13801 ggcctgcaga acaacgtgat cgaccgcggg ctctacgccc tcatcctggc gacggccgtc
    13861 atcaccatga tcgtgacgcc gtttctggcg atgctggccg gcccgctcta ttccctgcgc
    13921 aaccgcttct tccgcacgga gcgctatcag accgtgaacc tgccggcggg cggccttacc
    13981 gaccacgtgg tgatcgccgg cggcggccgc gtgggcgcca ccatcgcggg catcctccac
    14041 cacctggggg tcgcctgcgc ggtgatcgac caggacttcc gccgcttcga gcaggcgcgc
    14101 ggcatcggtt gcaccaccgt cttcggcgac gcaacccagg agccggtcct ggccgccgcg
    14161 ggcgtcatgc gtgcgcggct gctgatcatg acggtgccgt cgatacagtc ggcgcgcacg
    14221 atcatcgcga ccgcgctctc gctcgcgccc gggatcgccg tgatcgcgcg gggcgacagc
    14281 gacgagcagg cggacgagct ctacgccatg cgcgtccgcg aggtggtgcg ccccgagttc
    14341 gaggccggcc tggagatcat acggcaatcg cttctggcgc tggattatcc ggccgcggcg
    14401 atccagcagt acaccgacga cgtccgccgg tcgcgctcct cgccgctgct acgcgccaac
    14461 gccgactact cgctcctcgc ggggctgcgc agcgccgcgc acctcctgga gctcgcctgg
    14521 atcgacatcg ccccggagag cccggccgcc gggcgctcca tcaaggacct cgccgtccga
    14581 tcgcgcaccg gcgtgtccgt cgtgggcgtc ttccgcgacg gccttttcac cccgaacccc
    14641 gacgccgatt tttcccttcg cgagaacgac cacgtggcca tcatcgggac gccggcggga
    14701 aagaaggcgt tcgaatcgct cgtcgcaccc ggcgaggccg ggaatcaacc ccatacggag
    14761 gcaggcacat gaaagcgtta ttgtgcatcg tatcgatcgg cgttgcggca ttgctcctga
    14821 ccgcgggcgc ggccgcgccg aaacagaggg ggaccgcccc ccggccgtcc gcatgctacg
    14881 tcccggtcta ctccgccgtc cactacggcg accggcagca tgcgatgaac ctcgcggtga
    14941 ccctggccat ccacaacacg gacctgcgaa acgccatcat gataaccgcc gtggacttct
    15001 acgatacggc ggggcgtaaa ttgaaaagct acctcgcgac gccccgcgcg ctcaacccgc
    15061 tggagacggt gcagtactat gtcagggaat cggacacgag cggcgggacc ggcgcgaact
    15121 tcatcgtgcg ctggcgggcg gcatccggcg cgggcgctcc gctggtggag tcgatcatga
    15181 tcggcaccca cggtcagcag gggatctcgt tcacctcgcg gtgcgtgccc attcccgact
    15241 gacgacgcac ctcgatcatc acgcgcagga gacggcaatg tccgatatgc tcaatggcaa
    15301 aacgatcctt ctggtcgagg acgaggcgat catcgcgatg gacgaggcgg cgcttctgga
    15361 gcggcgcgga tgcgccgtca tcgtcgcctc ctcggggagc gccgcggttg acgccgttct
    15421 gcggaatcct cacatcgacc tcgtcctcat ggacatcgac ctcggcgcgg gcgtcgacgg
    15481 gaccgcggcg gccgagcgga tcctcgcaca ccgcgaggtc cccatcgtct tcctctccag
    15541 ccacaccgag ccggaggtcg tggagcgcac cgagggcatc acctcgtacg gttacgtggt
    15601 caaggacagc ggcgagacgg tgcttctggc ctccatcaaa atggccttcc ggctgttcgc
    15661 ggcgcgcacg gcgctcacgg aaaaaagcga cgaggccgag gccgccaacg aggagctcgg
    15721 cgcgacgatc gaggagctcg aatcgaccaa cgaggaactc gcgcgctcgc aggaagagct
    15781 gctgcagctc acgacgctcc tggaacaggt gttcgagcag tcgcccgttc cgatggcgct
    15841 ggtgagcatg cccgacgtca cgatccgcat cgcaaaccgc gcctgccgcg aattcctggg
    15901 catcctggac gagccctctc cggtggggac ctcgcttccg gacttccgcc ccaccttcga
    15961 ggaccgcgac atgagcggcg cactgggcgc ggtgggcgat ctgcccctcg cccggtcgct
    16021 gcgcggagag cggaccgtca acgaggagcg gatcatcgtg cggaaagacg gcacaacccg
    16081 ccgcgagctg gtgaacgccg tgcccatcgt gaacgagcgg ggggagatca tcgccggata
    16141 cctcacgctg atcgacatta ccgagcggcg gaagattgag atgatgttcg aggcgatctt
    16201 ccggctctcg cccatcgcga tcgccctcac ccgcttcgag gacggacgga tcgtgcaggc
    16261 gaacgatcgc tactgcgcca tgacgggata tcgcctcgac gagatcatcg ggaaaacgtc
    16321 tctcgagctg aatttctggc cgcaccctgg ggaccgcgat acggtgaagc ggatactcgc
    16381 gaagaacggc tcgtttcggg accgcgagat cacctttcgt cgtaaggacg gcagcctcta
    16441 cgattcgcag caatcggcgt cggtgatcac catcgacggc gaacgctaca tcatcgccgc
    16501 caccaaggac ctcagcgacg agcgggcgat gcgcgagacg ctgcagcacc gcgaggcgct
    16561 catcacggcc atcagcgaca acctcccggg ggccatgatc taccaaatcg accgccgccc
    16621 cgatgggacg cgcgtcatca cccacctcag cgatacggtg cggcggtttt acggctgctc
    16681 gcccgaggag gccatcgccg acccgacgcg catctacggg cgcgtcgccg acgaggacac
    16741 ggcccggctc ttcgaggccg aggaggaggc gaaccgatca ttgtcgcgtc tcgacatcga
    16801 ggtgcgcatg atcgatccgt cgggagcccc gcgctggtcg cacttcgttt cgacgcccgc
    16861 gctgctgccc gacgggacca cgcgctggga cggcatcgag gtggacatca ccgagcgaaa
    16921 gcgcatcgag gacgagctcg cccgctcgct ggccgcgcag gagaccctgc tgcgcgagct
    16981 ccgacatcgc gtgatgaaca gcctgtcgat catcggctcg ctggcgggca tggagggccg
    17041 cgcgaccgag agcgcggcgg tgcgggcggc cctgctgtcg atgggcggac gtatccgttc
    17101 gctcgcggag ctctacgaca tgctcccgct gtccgcggcc ggcgagatac ggctggacct
    17161 gtacctgcgc cgcatcatcg acgcgttcgc ccgctccatc ctgcacgact cctcccgcat
    17221 cgtgataacc gcgaccggcg acgagatatc catcgacgcc gaccgcgcga cgacgctggg
    17281 actcatcgcg aacgaactgg cgaccaatgc cgtcaagtac gccttccccg acggccgggc
    17341 cggcgcggtc aacgcaaccc tccgccggga cgggatcgac attgtgctga tgatgagcga
    17401 caccgggatc ggccccccgc ccggattttc actctccggc ggcaccggga cgggcatgtc
    17461 gatcgtgacc atgctggccc ggcagctcga tgggagcgtg tcgttcgagc gtatcgacgg
    17521 acggaccgtg ttcacggtgc gcgttccgta cggcgcataa cgcgcagacg acgcggtttc
    17581 ctatcggccg gcgagcaggt cggggtaatc cgtgcagtat tccttccgct ggcattcgac
    17641 gcagcgcgcc cccatccaga cctcgtcgaa ctgcgccatg atccgcttca ccagcgcgcg
    17701 ctcgtcggtg accacgcccg cctcgaagtt gcgccgcgcg tcgctcttcg cgcccatccc
    17761 ggcgccggtg aggttcgcgc tgccggtgta ggcgaagcgg ccgtcgacga tcacggcctt
    17821 gaagtgcacg cgcgggcact ggatgcgctc caacccgtcg atgaggttgg gatggcggtc
    17881 gaaatcgtcg cggaaatgcg gcccaacctc cttcgcgtgg agcagacgca ccgacacgcc
    17941 gcggtcgatg agcccggaga ggaccgcaag gaacggcacc atgcgcgtcc cctgcgacac
    18001 gtagaggtct ttgaggtcgg cggtggcgat ccagagaaag cgctcggcgc tcggcgccgc
    18061 gccgaggatc acccgctcgt agatttccct gtcggtgatg aagtcgatca tcgcacgctc
    18121 cggcgcatcg ctccgagcag atcacgtcgc atgcgcgctg tcaacgattt cccgcgccgc
    18181 acgcgacacg gcccgccgcg gacgcgataa gcggattgac aggccggacg ctttccgctc
    18241 caatgcgcct atggcggatc aaccgtatct gttcgccgtt ctcgtcgttg gggccgcgat
    18301 cgcatatgtc gtctacggcc tggtgcggaa caaatccccg cgcgaggcat gggaggcgct
    18361 cgccggcgtc cggggcccgg gcgcagcgct cgcggaatgc gccgtgacgc ccggagcgcc
    18421 gttctcgctc acgcaccgcg cgtcgcgcgc atcggtcctg cgggcctttc tggcctgcga
    18481 gatcgcctat cgcctcgatg gcgcggatgg gatcgacgac gagcgcgcct tcggaattgc
    18541 ggtacggatc gacatgggcg agccgaccgg tccgccgcgc agcgtgcgcc tggggatcgg
    18601 ccgcctcgcg ccgcccgacg tgatcgcgtt caccggcgtc atgcacggca cgcgtttttc
    18661 ccgcaccggc ggggagtgcc gcttccgggc cgtcgtcccc atcggccgca tgcccgtggc
    18721 ggcaggcgcg gaggcggtca tcgcgggaac ggtcgaggcg agcgcgaaca ttaccgcgac
    18781 cgcgatcaca gtgctcatca cgacctgacg acggcgccga gcgcgcccat accaccaatt
    18841 tgacaggact ccgaccgatg atccgcataa ccgaacgcgc cgacattcgc gccgccgcat
    18901 ccgtcctcgc cgaggccttt cgggacgatc cgctcttcgc gcatttcttc cccgacgcgt
    18961 cccgacggcg tgatcgcgcg gtccacacct tccgcttcat cgtgagccat gccgtcggcc
    19021 gggggcgcgt gtatctctcg ccgcgacgcg atgcggcggc cgtgtggctt ccgcccggca
    19081 tttccgagcg cggccttgca gaccagatca gatatggggc gctgcgaatg ctcgtccgcc
    19141 agcacccgcg gtcgatcgtg cgccagatgc gcgcgtccga cttcatgcac gcgatccacg
    19201 agcgcttcct ccccgcacac cgctggtacc tttcaacgct gggcgcgctt ccttcggcgc
    19261 gcgggaccgg cgccgcgtcc gcgctcgttc gcggcatgct cgacgagatc gacaccggcg
    19321 ctctcccgtg cgccctggac acgcacaacg ggaacaatat ctccttctac gcgcgcttcg
    19381 gcttcacggt ggtccatgag agcgccatac ccggaacctc cgtccgccac tggacgctcc
    19441 tgcgcccgac gcgcgcccga tgagcccatg ccgagcgcac cggccccatc gatcacgatc
    19501 ataagcggcg ggcagacggg cgtggaccgc gcggcgctgg acgccgcgat cgcgctgggc
    19561 gtcccccacg gcggctggtg tccggcgggg cggctcgcgg aggacgggac tcttcccgcc
    19621 cgctatctcc tcaccgagac gagatcccgg gactactcgg tgcgaacgga gcgcaatgtc
    19681 cgcgactccg acgcgacgct catcctgaca tggggacctc cctcgggggg aacggcctat
    19741 accatcgagt gcgccatcag aatgaaccgg ccgtatctgg tggtcgacat ggaaggagag
    19801 agcgccgatc cagcccgtgc ggctaattgg atgcgagacc gggacatcgg ggcgctcaac
    19861 gtggcgggtc cgcgcgagag ttcgcgaccg ggcgtggtgc attcgacggc gatgcgtttc
    19921 cttgtcgcgc tcctggaagc gctgctgcac caatagtccg gtaccgttag ccgcgatccc
    19981 gcacaaccga catcacggcg cctcgcgcgc catcagccat tctcaaacga ggtcacgtat
    20041 cgcggaacgg ttcgattgct cgggcgcgtg aggacgatct gaaggtcatt gacgatcctg
    20101 ctctcgaaac ctgcttcaca gattgacaaa taataacgcc acagccgctg gaacatttgg
    20161 tcatagccgg tctgctcgag ccgctccgcc accgcgtcaa aacgctcccg ccatgcccgc
    20221 agggtggtcg cgtaatgcgg cccgatgttt tccacatgct cgacgaacaa tgtggaccgc
    20281 ttgagcatcg cgttcacgag cgcgccgagc gacggcaggt gcccgccggg gaagacgtac
    20341 ttctggatcc agtcggcccg gcggcagtac gcgcgataat gatggtccgg cgtggtgatc
    20401 acctggacca ccgcgattcc cgagggcgcg aggcagcgct ccagcgcggc gaagaagtcc
    20461 ccgaggtacc gatggcccac cgcctcgagc atctcgatgg atacgatctt gtcgaacgat
    20521 ccgcgcacgg cgcggtaatc gcagaactcg acgcgcacgc gatcagaaag ccccgccgcc
    20581 gcgatgcgtt cgcgggccat cgcaagctgc tcgcccgata tggttatgca cgttacgcgg
    20641 cacccagttg ttcgcgccgc ctcgatggcg aacccgcccc atccgcagcc gatctccagg
    20701 acgtggtcgc ccgcggtgat gcccgccttg tcgatgatgg cgtggatctt gttccgctgc
    20761 gcccgctcga gcccgtcttt ctttgagcga aagatcgcgc atgaatacat catgctacgg
    20821 tccagaaatg acgagaagaa gccgttgccg agatcatagt gggcgctgat atttttgcgg
    20881 ctgcggaaaa tactgttcgc gtgcaggcga tggacgaggt actgcgccac gcgcgagaga
    20941 cggtttgcgg aacgcgccgc gtcgcccagg gcgtcggtgt tatcgataaa acaattgatg
    21001 acgcccacca cgtccgggct gtcccaaagg ccggcggtga acgcctcgcc aaagccgatc
    21061 tccccgccaa agaggaccct cgtgaaaaac cgataccgac gcacctgcag caccgcgcgg
    21121 tgcgaatcgc catgcgaccc ataggatcga ctttccccgt cgggcagcac caccagaagc
    21181 cgcccgtgcc gaatggcggt cagatgggac aacaccatac gcatcgcgat acgctccatc
    21241 aacgtgggcg attcgcactt cgcaaccgta tcactacaat tctcatgttc catacctccc
    21301 ccttgcccgg atatccgggc atccgttgcc gcaccatcac agcgacgaaa aaccacacgc
    21361 ccgatgcgac cgtggacgat acagcgcgac gcttattccg ggacggtgtc gcaggattgc
    21421 gtcatgcggg aacccgcgcc gcatgaatcg acaccgccgc aatgcatcac caataggaag
    21481 gacaaaaata cgcgatgacc agttgcacca gaatattgaa aatgaaacaa gacggaaacg
    21541 caagaaccgg gacaacaact cattctataa attccggcgt tgttggccgg ggtggaacat
    21601 cccgtacggc gggtaaaaaa acaattgcgc ggaatctcct ctcgtttcac agtatgatcc
    21661 ggcgcggctt cgccgcaccg cgacacccca tcgtggaggg accgcatggc ctgcgaccgc
    21721 atggaaaaga acctggcgat gtgcaactgc tcgtacgagc cctgcagccg caagggaaaa
    21781 tgctgcgagt gcatccaata tcaccggcgc tcgagcgagc tgcccgcctg tttctttccg
    21841 gacagcgtgg agcgcaccta cgaccgctcc gtgcgccggt tcatccagtg ccagaagtaa
    21901 tgcccatcac gcgcacggcc gcccgcatca tgctgttcgc ggcgtgcttc gccctgggct
    21961 gcgcgtcgtt tcgcgattcg cgcttcgtgc cgcccttcga cttcaccccg ccggcgcgcg
    22021 acgcgggcgc gctgctcatc atcaaccgct tcgccgggcc cgatacgacg gccggctcca
    22081 tcgtctccga ggccgaccgc gaatccttcg tcgcgaccgt cgagggggcc tgccgcgacg
    22141 cgcggctttt ctcgcgcacc gtcacgcagc ggcgcgccgg gcgggagcgg gacgcgggct
    22201 acgcggtcat cctggaaacc gagcttgcga tctcggaaaa cctgcacccg aaatcggcgc
    22261 gggtgctcaa cacgttcctc ttcacctggc tcgaccggcg gcgcgtcacg ctgcgggcga
    22321 gcgtatacga cgggggcggg acgcgccgcg gcgagtacga gatgagcgag cgattcgaga
    22381 cgatcggctt cctcctcctg ccggtgttcc cgctcctggg acgctccgag cgcacggtga
    22441 aggacgaggt ctttcgcacg ctcgtcgtgc gcgtcatcaa cgcggcgcag cgggacggcg
    22501 cgtttgcggc gccgaaaccg aaagagtgaa aggacgggcc gttttgcttg aagtcatcgg
    22561 gagcaaagcg aggcgatcgg aattatcaaa catgaagcga atgaacatct ggcaacacac
    22621 cgtaatccaa tgcacgcatg tgcctcaaac ggtttcattc gcctaaaaaa gtatccggaa
    22681 cgcgttcaaa caaccaacgt tcgccccacc aatacttttt ccaacacctt cgacatggcg
    22741 atatccgatg taatgcatgt gtcaggatca gaaaggctgg ctgaagcgat ttgaatacta
    22801 tcgagggtcc ttatgttatg ggcttctatg atcgatatcg ctctttccac aaggatttcg
    22861 ctaaaaggaa caatcgaata atggcgaata tcctgtcccc aataccgctg agccttgtca
    22921 aatgtctcct gatcaatact gcgatcggcg acttttcggc ataatgctga atacatctca
    22981 atcggcgtta ttggagaaat gcatatggca ttcccactga caaaaaaccg atcaacttga
    23041 tcgcttcccc gctcttcaac gtatcgcttg agcagactgg atgtgtcgag aaaaactacc
    23101 atcgtgcctc atttctgagc tttaccacgg tatccgaagc catttcccca atggaacgac
    23161 cacgagatcg cacatcaacc cagcggactt cttctccatc ccgctctgat tgatatttag
    23221 caatgagata ctcggcataa tcgagtatct ctttttttat ctcatccggc atgcgttgaa
    23281 tatcttctag ttttatcatc cggcctccag caatcatccc ccttccgggc cacggaatgc
    23341 ttcccccgtg atgagtatac tatatattac ggggaatgag taaatttttt ttattcattt
    23401 tacaaattcc agattgcatt ttcttcccca aatcgcccct tataaaccca atcatcaaac
    23461 gacactactc ctcaatcaag aaaaacgcaa gctcaatgag aaaatcgttg catactgagc
    23521 aggaccatcc aactacaatg gtgctgcaga acaagcgggt tgtcacgcga attggctgaa
    23581 attacatgat caacatcacc atcgacgaga aacgcgaact gctggagggg atcgaacggc
    23641 agtaccggaa catggccccc gacgagaagg acgaactgct cacggtcgcc atcatgctca
    23701 agctgtacga gaagaacaac ggcttccccg cgcgaaacat gaacgacctg gagatgtggt
    23761 actcgctcaa taaggcggcc gtggatatgc taccggaccc ggaggaaggc gacgcgggcg
    23821 tataatcgtt gacacgcccg ataatcatcc gtatccttgg agcacgcggg ctcgccgccc
    23881 ggggaggaat cagatgacca tttctcgaag ggtatcctac accggcctgg cgctttccgg
    23941 cctgtacgta ttcatcgcaa cggtggccct ggttctctcg tcggactgcc ggggcgaggg
    24001 ctgcgcggtc cagctcttcg tcacgttccc gtggttcttt ctctttctgc gaagctcatc
    24061 gacgatcatg ctctttgtcg tatcggtgtc gctcaacgcg atcctgtggt atctgctcgg
    24121 gagcgcgatc gggcggctcg gaaaatggat cgtgtcgcgt ctggggcgcg gctgattcca
    24181 acggggagcg cgcagcatac ggaaagcggc gcctctgcga gccgacgccc cacggttccg
    24241 ctacttcgcg ccgtgcttca gtaacacctg ctcgacgctg gcttgccgcg tcttcctcgc
    24301 ataggaaagc gcgctctcgc ccgagaggtc gcgcgcgttc acgtcggccc ccgcaccgat
    24361 gaggagctcc gcgatcgcgg cataatcggc gggaccggcg gcgccgcgcg cggtgtcgaa
    24421 catcttcagg agatcgattt cgcgggcgcg cgcatcggcg acgatcatga gggccgtccg
    24481 gccgcgccgg tcggtctcgt taagattcac ccgggcattc acgatcgcct tcgcggcggc
    24541 gttcgatccg gacgcgcacg cgatcatcag gggagtccac cccgtcgact tctcgcgcac
    24601 cgcgagcgat atgccgcgct cgacgaacag cgcaatcacc cggtcccggt cggcggccgc
    24661 ggcctcgccg atcgcgcttc gcccgtgctt gttctccatg gcgatatcgg cccccgccga
    24721 caggagaata ccaatggagg tgagcgcgtt cccccgcacg gccaggatca gcggggtata
    24781 gccgccggca tcctgcacgt tcacctcggc cttgtgacgc accagcgccc tgacggcatc
    24841 ggcataattc cagtacacgg cggggaacag cgccgtccgt tgttccgatc cgcatcgcgc
    24901 attgacgtcc gcgcccgccg cgagcaccat atcgagcatc gcggcgggat cgtacgcgcc
    24961 ctttccgagc ttccccccga ggtcgggccg gtccaggagt tcgtataacg ccgtacgccc
    25021 gttctttgtc gcgatgttca catcggcgcc cactgcgagc agcgtgcgga tgacctcgat
    25081 cttatggcgg acctttgatg catcgctcgc ctgtttaacg gcgaacacga gcggcgtctc
    25141 gccgtcgttg gtgcgcgcat tgacgtcggc cttatgctgc agaagaagct tcaggacggc
    25201 gatatcgtag ctctcgaggt acaggttctt gatgttgtga tgaagcgccg ttttcccctc
    25261 gttgtcccgc accgacgtgg atgcgcccga ctcgaggagc gcgcgcacca ccgggatccg
    25321 ttgcgccgag tggatgccga cgtcagcggc gaccatgagg gcggtcatcc cctccccgct
    25381 tttgatatcg ggcgaggcgc cgcgcgcgat caacgcgcgc acgaggtccg tcttgttgta
    25441 gaacgcggcc cacatgagcg cagtccagtt ggtacggatc ggatcgatct ggccgcccct
    25501 gacattgggg tcggcgccgt atttaaggag cacgagcgcc gtatcatcac tgatgttcat
    25561 ggagcgactc gccgccatga gcgccgtcgt cccccgcccg tcacgcgcgt cgggaggcgt
    25621 gcccgcatcg agcatggcct tcaagggccc cacatgacca taagaggccg ccacgtgcag
    25681 gggcgtccat ccctggcctg gcgccctacc cgtcggcttc gctcccgcct ttatgagcac
    25741 gatcgcggtt tctggagcat tgtacgccgc cgcataatgc agggccgtcc atcccgagcg
    25801 aaaggtgtac tcgccggtcg tttcattcac gtcgacgccg ctttcgatga gacatgcagc
    25861 gacgtcgctt cggttgtaga ccgcggcgta catgaggggc gtcaggggat tgttcttatg
    25921 atcctgtcgc gatgatgtaa tggagggatg cgtgcgaatc agctcgatag ccgctttgga
    25981 gtcgccgtgt tcaatcgcgg taaacaagcg ccccccgaac atggagcagg aaaacgaggc
    26041 cagcgctgcg attgcgcata agatcatgtt tcgcatggta tcaccgcacc ggagatgcat
    26101 gcgcgcattc ctagcaccgc gattgatgaa cgtcaagaac ttatgcgctg gaatgccgca
    26161 tcacgccatt cagatgccgg attgttccgg ggacaaacca tggacctgcg tttttggcca
    26221 tgtgagcgcc cagggatcct cgcccaggcg tatgagcgcc gccttgaatt catcggacag
    26281 aaagtaacgc ccctcatcgg gcgggcagac gaagcgatgc cggaccccca tcaccgagaa
    26341 gccaagcgcg taagcatgga ggtatcctcg atccggctct atccccgcca tgaccttctt
    26401 gcaataccgc gcatcaccga tcaccggcgc gcctaccgac ttgagcgcca cccggacctg
    26461 atgggtgcgg ccggtataca gccgcagcag atagagccgg agacccggct ggaggaaggt
    26521 gctaaaaaac ctggtgaccg ccggacgctc atgcgtgcgc gtgagtatcc agacgccgtc
    26581 gcggcccttt ttcatgtcgc cggagacgat tccctgtttc tttttcggat ggggcccgcc
    26641 gaccgccagg tagaactttt ctatgtgccg ctcgcgaaac tggcgggcca gctcgcgcac
    26701 gaccccgcgc gtccgcgcga agaccaaaag acccgaggtg agattgtcga ggcggtgcag
    26761 cgcgtagagc tcgtcgatgc ccatccgctc gcggatcgca tcgtccaggg acggcgcacc
    26821 gtcgccgccg tgaaaggaca cgccggcctt tttgcttatt acgatgaagt catcgcaggt
    26881 atggatgagc gtaaacattc ggcgtcctcg acgccaggtg aatcaggatg atggaattgg
    26941 caagcagtat tgcgggca
//
LOCUS       DAORHF010000023        26899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1109_length_26899_cov_5.521271, whole genome shotgun sequence.
ACCESSION   DAORHF010000023 DAORHF010000000
VERSION     DAORHF010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1109_length_26899_cov_5.521271"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKJ16_03565"
     CDS             complement(<1..475)
                     /locus_tag="PKJ16_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46095.1"
                     /translation="MAANGTNGNRATTRERILVVDDEQTIREILSKYITQLGYEVVTA
                     EDGMHALEKLAALPFDLVLTDLKMPKMDGRELLKRLAEAYPDIPKIVLTGLGTDEDII
                     LALKTGAYDFLTKPIIDFEILHHALKRALERKRLNDERTRYADQQTHITEIVSMLN"
     gene            complement(570..1295)
                     /locus_tag="PKJ16_03570"
     CDS             complement(570..1295)
                     /locus_tag="PKJ16_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV46096.1"
                     /translation="MTAPTIAEAKRTAIALSRATPRPLFYREKTHELDRARALSRNDR
                     AAHFARDSIRTEGSVLGHDFYHAHRVAHDAAAIVYCERGDDDDGLARLALVAGYLHDI
                     RRNMRDHSARGAEAVVPLLANILPARDIAMVAVAIRDHEAFRERVAIDDDDARLLADA
                     LYDADKFRWGPDNFTYTIWSMARSLAISVDDLVRHYQAGIAGITRIRDTFRTDTGRRY
                     GPDFIDRGLELGGALYAYLTERR"
     gene            complement(1312..2205)
                     /gene="hisG"
                     /locus_tag="PKJ16_03575"
     CDS             complement(1312..2205)
                     /gene="hisG"
                     /locus_tag="PKJ16_03575"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HNV46097.1"
                     /translation="MSETILKLGIPKGSLENATIDLFQKAGWRITSSSRNYFPSIDDP
                     EISCSLVRAQEMARYIESGVLDVGLTGLDWILENNARVEIISDLIYSKVSMRKARWVL
                     AVPEHSPISSVEDCANKTISTELVNFTSRYFAERGIPVKVEFSWGATEAKVVEGLVDA
                     IVEVTETGSTIRAHGLKIIHTLLETNTKLAANVESMQDSWKRNKIGQIALLLKGALNA
                     GNLVGIKMNVPTANLDAVIALVPSLTAPTVSRLHNTDWFSVESVIDEKTVRGLIPRLI
                     NAGADGIIEYPLNKVVSKGDC"
     gene            complement(2202..2585)
                     /gene="hisI"
                     /locus_tag="PKJ16_03580"
     CDS             complement(2202..2585)
                     /gene="hisI"
                     /locus_tag="PKJ16_03580"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HNV46098.1"
                     /translation="MIELDFSKLGGLVPVIAQDHRTGEVLMMAFMNAEAWDLTRSTGI
                     VHYYSRSRNKLWKKGESSGNIQEVKEIRVDCDNDCVLVKVNQIGEAACHTGYVSCFYR
                     VLEGDTVRIDGTKVFDPETVYGEKK"
     gene            3250..4227
                     /locus_tag="PKJ16_03585"
     CDS             3250..4227
                     /locus_tag="PKJ16_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46099.1"
                     /translation="MRRMFLVAVLMMMTPAFADDDANERLLGNSRNGSVPGIRMALKD
                     GAKINEAHDHEYNTALMLAAGGGHLEAVKVLVDNGADVNKVSEGSSYSTALIEAGDTG
                     SPQVIAYLLSKGAKVNEATSYKQSALIRAAYSGKAANIRALIAGGADVNMMDDAGAAI
                     HAASMNGHGDAVTALIAGKANVNLPGSSHYVTALMLAAEKGHANVVKLLLDAKADVNM
                     KSVVKRQALEYAAEKGHAAIVRMLVAKGADLNNQNSENGRTALYAAACKGHVETVRIL
                     LAAKADPNIKSTNVDYTPLKCASENFERIDEAQHRQLMDLLRKAGARME"
     gene            complement(4436..4864)
                     /locus_tag="PKJ16_03590"
     CDS             complement(4436..4864)
                     /locus_tag="PKJ16_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46100.1"
                     /translation="MDFAAVCGFLFESFEKEHIRFALIGGLALHASGVTRTTEEVDFL
                     IARRRYALAMLDRSIPLPVLGGRFTARVVAPEDLIGLKVQSSINDPSRADHDRADIRN
                     LMRNNLKNLDLDRIREYYALFGKEEEFSQLLAEMKRASNT"
     gene            complement(4873..6360)
                     /locus_tag="PKJ16_03595"
     CDS             complement(4873..6360)
                     /locus_tag="PKJ16_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV46101.1"
                     /translation="MRQKRHRTPIVRILAGLCLLFGVVSPAHRAAAGPFIKINSIDSS
                     NFPAVKVHLTATDGAVGHLSDLDDEHLSLIEDGYRINYMTMLDREASRDLIYLVFSLD
                     TSKSIHAKNFAKLKTIARDIVNASTDRDRIAVYKFNSEVALLSNFTGNRADLSKLIGR
                     LEAHGKKTLLYNSLYDSLDLLSRANGPRKAVIVFTDGRDEGSSVSAGDVIKFARDLQI
                     PISFVAVDGDRYVRELSRIAKLTGGRLYRARQVTDVGEIYRTLVRAIKNQYVVQYKSM
                     LPADGRAHTLEVRLKYGTLRDRDTAPFTVKKRLFDIAMPTALEIALAILIALLVLGMI
                     VFMVIFLRRNGLVPARKTERAAAPRAESSEAGDYHVAIDLDEQERKSGDRVITATDPE
                     YVYAKAWLLEKDGPEVGKKFPIFWDEVTLGRGDDNAIVIKDDAVSLNHAKIKRVKNAY
                     TLYDLVSDNGTFLNDNKLLRPKTLYDWDEIRIGRTVLLFRGSKNA"
     gene            7240..7890
                     /locus_tag="PKJ16_03600"
     CDS             7240..7890
                     /locus_tag="PKJ16_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46102.1"
                     /translation="MNGKGIRRITIIVFCAFILNGCRSFDMVENEKQKHAIDQQVPNM
                     TLVVNKPSIKACFANWQHSIVTAEVGPIGFPQMKEEGLTSDNLESYVETVIRNNFIMQ
                     SNNHSNKYLSVIVRNIDFSQNWWWEPVTFGGILGILGIPVCSQTLIIEMEAAVLQQNG
                     NILKIYKTKAEKTAYGALYWGYMWIGRASFCMALNNACADLREQIRRDADYLKSVK"
     gene            8057..8182
                     /locus_tag="PKJ16_03605"
     CDS             8057..8182
                     /locus_tag="PKJ16_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46103.1"
                     /translation="MVDGSEIRGEIVNQNRTAITVKTKYTTMTIEKKNIREIKYK"
     gene            8297..8884
                     /locus_tag="PKJ16_03610"
     CDS             8297..8884
                     /locus_tag="PKJ16_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46104.1"
                     /translation="MASLFNGCVSPMWQAMKDGDIEKVKTFLEQGEDVNKAAFGMSPL
                     TFTAYYGKTDLAKLLLDKGASIDRQSTNSLTQQTATPLFWAIDRGNHDTAKYLISRGA
                     SIDVAIAAFDDLIIKNPSGNTGAWKQNSALLHEWKKTRPAGSTAGSNENTIFMNDGSE
                     IRGEIVNQNRTSITVKTKYTTMTIEKKNIKEIKYK"
     gene            8967..9743
                     /locus_tag="PKJ16_03615"
     CDS             8967..9743
                     /locus_tag="PKJ16_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46105.1"
                     /translation="MRVGTIIAVIVISTIILITGCVSLYTAVEDNNVNQVKILLEKGT
                     NPNESYSVHRAAWKGNYEIVKLLVDKGADLNVKYLNKGPLVYAVWGKNNEIIKLLLDK
                     GTNTDSAIYQAVNESNINVIQMLLDKGADLNRNIDGMPLISQYAFKGEYKTSNAEFIV
                     TRGANVIDAIDRLEQEMVNHPNETAKYQLAITRLNRLKSLNVKASKSKPKESENTIHL
                     NDGSEIRGAIVNQSRTNITVKTKYTTMTIEKKNIKEIKYK"
     gene            9882..10778
                     /locus_tag="PKJ16_03620"
     CDS             9882..10778
                     /locus_tag="PKJ16_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV46106.1"
                     /translation="MRILAVHGKATLVSGAVTTDLKPDATVNAGDTIVTEKQTTVDLY
                     AGNQSAVRIYENTKFTVNARTVDPGGNITNATMGLTGGRIFVIIKKLAMDRAISVSTP
                     TYVIAVRGTEFSVNTFTEKDRQLTKLDVVEGKVEIKSTTKPELADTLVEGDTITLAPD
                     KKEKEKAKLDAPAIEKLKQDAGTLREKTTEYEKKAGVASAPDAASQKPAGDDKAATVA
                     DAGKKPDEKKSAAPADNKPPVLKTEKQIREYYNKLERVNMEDGSVLIGAVIGQSGTHL
                     RIHTVNGIISVPKTAVVNVVMQ"
     gene            complement(10789..11721)
                     /locus_tag="PKJ16_03625"
     CDS             complement(10789..11721)
                     /locus_tag="PKJ16_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV46107.1"
                     /translation="MIRNHVIGLFCVGAVALCAFAVIGIAATDDIAIPTPFPGRYIDI
                     EGETIRFHQAGAGRDVLLVHGLPGSIEEWEPVFGLLATRYRVIAYDRPGQGFSSARSD
                     DFSIQRNATLALALITRLSLRNVIAVGHSYGGGVILALAVHNPPEIAAYLSIAGSSRP
                     RGGAMAFSHITALPVIGRGIAACDARVRAGEMVAEGIRYAFHPNDSSLPADYIETRLR
                     IWLQTKVLVSMAKEDVAYDAGMGKLTPLYSKIRKPLVILHGDRDRNVPVADSVRLHEA
                     VPSSRLIILRNTGHMAQYANPAAVVTAVDSLGRR"
     gene            complement(11755..12603)
                     /locus_tag="PKJ16_03630"
     CDS             complement(11755..12603)
                     /locus_tag="PKJ16_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46108.1"
                     /translation="MSATLIILLAVLAMGALAVACASRRAVVVHQAPCGDATLRVEKR
                     REMVELAWQDRFDLALMRPGANTAVRLTGNVGVLPRAKRYRDMIAIADFREGSRPAMG
                     ELFTTHYISPRRITREEFDEVCRVYRHRRAEWSVIAPSIGLLVYGEQRMFHEVFDCGS
                     GLYLYTDLDGNILITDDPAYESLSRIAERRHMNHVAFFTDDNRLIFRTGRIVSGVEND
                     LFGRPSKRVAYSGIAGIADYANHVDVRWCDLLYETDRTGINASLLRSAKNRDGKRLDE
                     IFRAAD"
     gene            12643..13449
                     /locus_tag="PKJ16_03635"
     CDS             12643..13449
                     /locus_tag="PKJ16_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HNV46109.1"
                     /translation="MRTALRGTMHSAEIIDARWSREPTLGTVLAQSTRCSLCLDIFVR
                     PWHITTMENALVLLDLFGTLAFAISGAFKAVKYELDLLGLMVLATMTGIGGGIVRDIL
                     CGSLPPAALRNEIYFFVCIAGGIAVYFLAPRIARRWDIVLFADAVGLGVFAGIGGIKG
                     VDMGFGFMGVILLGTLTATGGGVIRDILVSEIPMVIRSDFYATAVIIGCAAMAAVNAA
                     GAPRVAVLGVCIGVTILCRLLAMRFDLSLPKVKSLPESPTQMTRRRRTKD"
     gene            complement(13479..13985)
                     /gene="mog"
                     /locus_tag="PKJ16_03640"
     CDS             complement(13479..13985)
                     /gene="mog"
                     /locus_tag="PKJ16_03640"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HNV46110.1"
                     /translation="MSVGVITVSDRSSRGEREDASGPAIREWAEAHGYAVARETIVPD
                     ERDAITRALVDYSDAGVELILTTGGTGFAPRDVTPEATCAVIDRPAPGFAEAMRAKSL
                     AITPHAMLSRAASGIRKRSLIINLPGSPKAVRENLGFIEAAIPHAIELLRGEVKDCGT
                     PPSDLPLR"
     gene            complement(13985..14344)
                     /gene="hypA"
                     /locus_tag="PKJ16_03645"
     CDS             complement(13985..14344)
                     /gene="hypA"
                     /locus_tag="PKJ16_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HNV46111.1"
                     /translation="MHELSVMSNILDIVVEHAKRNNATRVTKINLTVGELSDLVPEWM
                     QNYFDFVSKDTIADKAVLVIERRPAVLRCRDCAFEFALDREAFDFSCQKCRSANMEIV
                     SGREFTVNSIEIDGGGE"
     gene            complement(14373..14909)
                     /locus_tag="PKJ16_03650"
     CDS             complement(14373..14909)
                     /locus_tag="PKJ16_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46112.1"
                     /translation="MLARIALIAALAVSSPAIDFPRRVPADETLAVPGVGAERIVVGD
                     AAPVVIGRLGVPEAKAQFPERRELFRDVLLTPAPITLFFTAVYEYRTRRVAVFMDRDR
                     VTAIVARTTGRVTPESVSLARGVEYFIFTYGNAGMHPIAKPPANTLFLYPRLGIGIVD
                     DKNDDTIDMFIIFAPTAP"
     gene            complement(14925..15977)
                     /locus_tag="PKJ16_03655"
     CDS             complement(14925..15977)
                     /locus_tag="PKJ16_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46113.1"
                     /translation="MAPEPQNPSQFDSLDKKTRLIKSALQFTNGDMDKAKQMASGQYN
                     DVRVIKGKLSIESSMVYGIFIIFVNAANRYIMNLNALLVASSGVFEKSKIFDGWKTFY
                     GDYKKYVDREGDNALGSYDFTTHLIGAIEGYNLYDHLEVENLDEITEDLKDIFKKYFK
                     VDRVQCQIAIDPTNSLVIDAEGIPIEMPGSREGGADAREAAASVDQRMNEIESQAEHV
                     IEGKIIVSPIKGKYINDIKAGESLKVVLTRDDEISMSVAKMTNAITEDDEFLPMKARL
                     KAKIPLPEGGFVLYGVVAKNVLVKIIEEEDVKIEMDQQAAEKSSEKSDESRLFLYVAL
                     LCGAIIIMIILLIRII"
     gene            complement(15993..16460)
                     /locus_tag="PKJ16_03660"
     CDS             complement(15993..16460)
                     /locus_tag="PKJ16_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46114.1"
                     /translation="MVSVKKISEGIKTTDDSLFGLLSSYKPHTAYFRILASTEEKRAV
                     LLITDNPRCWLRLPAERYAAYAPVRRQELGATKLSVKYLTAANIVRTFGPTLGVSLPK
                     KKTTVVNELDQGGCFLLEFPIDDLPLVLQAVRKIITLSPQRKYEIIETCLTEI"
     gene            complement(16554..17114)
                     /locus_tag="PKJ16_03665"
     CDS             complement(16554..17114)
                     /locus_tag="PKJ16_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46115.1"
                     /translation="MRLRISDIARELSFTSPDLITRRMGEAMYHLLREKIAGLAPDEV
                     LTLDFEGIQVIDTSFIDEFIVKLAYDSRGRTHPFYVKLSNITDIMQVNIDSVFSSHAQ
                     YTAGSFAVLTDRLMPNNSYYLGRLTPNERDVINYLSVNKSAHVSEIADFLSRSTLETA
                     EIVESLAGLRLIRQSGEPGAALITPL"
     gene            complement(17122..18018)
                     /locus_tag="PKJ16_03670"
     CDS             complement(17122..18018)
                     /locus_tag="PKJ16_03670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46116.1"
                     /translation="MNAIYKNAVVLPNNCKDLYSFEEVLSRTVSLADVRPDALDLSRI
                     SFIEPYSMLNLLLIGRNYLRATGDRLALINMPLTVHQYLARMDFLKKGVFTVPEPLDE
                     ELFLKRSSFSKTVIEITDIPNKERESLQAISAVVALFRTRANHILKYWIANSIVDYFV
                     TVISEICQNIFEHSLDSGYCAMQTYASGSEHIVRLAIVDSGIGIRESFARNKEIEFES
                     TAKLIEQALMTPISSKRPFGYGLCQVNTLMEKLKGSIFIRSENASIAVMYNRKHERKP
                     YTFMRNDLAAFPGTQLSISLSG"
     gene            complement(18236..18379)
                     /locus_tag="PKJ16_03675"
     CDS             complement(18236..18379)
                     /locus_tag="PKJ16_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46117.1"
                     /translation="MKEAIVKLLKYGWPDHIIANYLLVKPAVVKYYRKKLKITRNEYQ
                     GFY"
     gene            18534..19280
                     /locus_tag="PKJ16_03680"
     CDS             18534..19280
                     /locus_tag="PKJ16_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HNV46118.1"
                     /translation="MMTIDAGGPMFERRPDGSFVVTPQGPVRIGAVGRGVGSVDYTAP
                     AEAIRYAEKEILASITGIAPRNIIMLNQVHGDDIVIIDHAPVVDRPWHADADGLITRE
                     TGICLVIRTADCVPVFAYDPASRVLGAAHAGWRGCRAGIAAKLVRTMRERFDARPERM
                     ASYILPSIGPDSYSVQEDVAGLFPRDIIRKRGSMFLNLWTNIERGIVESGVPAENVFN
                     ARRCTLIERDSFFSHRNGDAGRNLNYAYII"
     gene            19430..20062
                     /locus_tag="PKJ16_03685"
     CDS             19430..20062
                     /locus_tag="PKJ16_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46119.1"
                     /translation="MKKEIVQLSNLVDSSKPKNIIAEVKKIFSFHYTERHFSRISKAA
                     TLAINLFEGRFPGYQSCNTVYHDLEHTMAALLGVARLLDGYLIRGRTLSEPLVINLFI
                     ATLLHDCGYIQESWDTEGTGAKHTDSHVERSVRFVEKNHEAFGIAAGDAPVIGSLIRS
                     TGLSVNMDDIPFRSPEERVAGALLGTADLLGQMSDRKYLEKLLFLYQEFR"
     gene            20126..20347
                     /locus_tag="PKJ16_03690"
     CDS             20126..20347
                     /locus_tag="PKJ16_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46120.1"
                     /translation="MTKKRMNESYGGLYKYARCHFQKRNGIDENLYLVAINRHIDYLH
                     RIIDDSSTNFRKKLKRADWVNHAHSFARH"
     gene            complement(20594..21082)
                     /gene="greA"
                     /locus_tag="PKJ16_03695"
     CDS             complement(20594..21082)
                     /gene="greA"
                     /locus_tag="PKJ16_03695"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNV46121.1"
                     /translation="MENSIDEKKKQLKEELETLRVEYKVELPKRIAEARAYGDLRENA
                     EYDAARERQAFVKARIDQISHQMSQLNDLSMSSIEESKIGYGSIITVLEKETNARVEF
                     TFVHPNEVNPSEGKISLSSPIGMALQNKTVGEEVVVNIPAGKKRYYIEKLTTLHGKTM
                     SV"
     gene            complement(21274..22656)
                     /locus_tag="PKJ16_03700"
     CDS             complement(21274..22656)
                     /locus_tag="PKJ16_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV46122.1"
                     /translation="MDTYDIIIVGAGPAGIFAARELTKRSPGTRVLLIEKGNRIEERT
                     CPKRQHDRCWSCQPCNITTGFSGAGAFSDGKLLLSGDVGGGLGEYVDRDTLAGLITYV
                     DAVYLANGADTRVYGRGSDDVITQLRQKAIESNLKMIECPIRHIGTEKCAEIYANLQR
                     ELIEDGVTILFKNPVKDIIVENGAVAGVIADREYRANRVILAVGREGASWLKELCARH
                     GIPTRVGGIDIGVRVEVRNEIMQEINDAVYESKLVYYSATFDDRVRTFCQNPAGIVAT
                     EYYEDGLAVVNGHSYKSDEFKTENTNFAILVSNYFTQPFKEPIEYGKYIARLGNLLSG
                     NKIIVQRYGDFRRGRRTTVTRLSRNNITPTLKDAVPGDLSLVLPYRIMLDIKEMIEAL
                     NNIFPGLSSDETLLYGIEVKFYSNIVETGSDFRTGLAGLFVAGDGAGITRSLMQASIN
                     GVLAARAIIA"
     gene            22876..23946
                     /locus_tag="PKJ16_03705"
     CDS             22876..23946
                     /locus_tag="PKJ16_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV46123.1"
                     /translation="MKPQVFFIRVAEGEDDGAVERKIGRLYSSAGFDPFVAKGETVAM
                     KTHFGEKGNTTHIRANHVRQIIKKIKAAGGKPYLTETSVLYKSKRSNALDHILLAIEH
                     GFGYDKMGAPIIMADGFLGNWEREIEINGEYYTSVSVAGDALAPDKLFIVSHATGHLA
                     SGLGAALKNLGMGLSSRKGKLNQHSQAAPAVKPTACTLCGKCIRWCPAEAVVERDGKA
                     FILTEKCIGCGECIAVCAFDAVGFEWDQTSEVLQKKMVEHACAICREKKDRIAYVTFL
                     TSMTRDCDCMTSKTKLIDDIGIVASFDPVAIDTATLDLTARFAGENLSRLSYPQYDPL
                     VQVRHAEKLGMGSMAYELVEVE"
     gene            23990..25816
                     /locus_tag="PKJ16_03710"
     CDS             23990..25816
                     /locus_tag="PKJ16_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46124.1"
                     /translation="METTVVANDLKSFRMRFILMTEGVGYFVMVPILLLYVCTNLSLN
                     LDQVVLMLLCSGAAVIVNSTFAIAHDLWTIRPVTGYFAKRLRGDDISPEEHGRAQQRF
                     FSLPYRHGINTVIHWITGLLLAIVPFVNLAELTAVQKFNMWILLAMMPPMGGMVYFFL
                     TEMLVQRLLNRGIFEKIEMKEFTLSFSFLGRISASIAIILSMPIFGLIGYFSIIIEGI
                     KPDYQFLSIKLAGIILLVIATSLVISFVLFKSIRDKIAIISDFLKKIGAGDLSAKKGI
                     IAVVDELTRINQTIYVMKKNITDMIYDIVRISDQLDESTAAISTVTDGFTEVTQNQAA
                     TMEEVTATIEQISAGMDQIATGAQDQVKSLRTLIDRMGDLTSVIREMERKIADASAQA
                     GEITVEARRGEESLAKMNESMENIGESSRKMTGIIGIINDISDKINLLSLNAAIEAAR
                     AGEAGRGFAVVADEISKLADNTATSVKEIDALIKATEREIASGTATVGDVVERISRIT
                     GGIGAMNETVTSIAAFMKTQIETNAIVDREVARVREKSEEIEGATQEQKTAIGDVVNS
                     ITRINELTQAISAGSEEIAANTKENADIADELKNKVHQFTIA"
     gene            26003..26518
                     /locus_tag="PKJ16_03715"
     CDS             26003..26518
                     /locus_tag="PKJ16_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV46125.1"
                     /translation="MKAYRKAYCEYYPIVYSVLLSRLRNGDDAEDICQEVFIRFLDNF
                     DSIESHRGWLLKAIEFEISNYYRKKSSRNDEAVDVAEFVNDINLAFENGFRDARIVIN
                     EAIENMENYDSEEDRTLFDLIAVYGFSYKEAGKYLGMTRWQAEYRYGQIEARIVDYLK
                     KMGIKEVFDLL"
     gene            26545..>26899
                     /locus_tag="PKJ16_03720"
     CDS             26545..>26899
                     /locus_tag="PKJ16_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46126.1"
                     /translation="MKELLHKYRLDHPVAPDSQKRMMAHMGPMYRRILKRTGRHSLIT
                     GIVLWIYFLLRRYGIVITTGKAIIVTIVLLVFSLGGILAFFMLQHRPDSAVNRKGGVV
                     DIRVDLDSAPVQPPRP"
BASE COUNT         5645 a         7510 c         8207 g         5537 t
ORIGIN      
        1 ggttgagcat ggagacgatc tcggtgatgt gcgtctgctg gtcggcgtag cgggtgcgct
       61 cgtcgttgag gcgcttgcgc tctagcgcgc gcttcaaggc gtggtgcagg atctcgaaat
      121 cgatgatggg cttggtgagg aagtcgtacg ccccggtctt gagcgcgagg atgatgtcct
      181 cgtcggtgcc caggcccgtc agcacgatct tcgggatgtc gggataggcc tcggccagcc
      241 gtttcaggag ctcccggccg tccatcttgg gcattttaag gtcggtgagc accaggtcga
      301 agggaagcgc cgccagcttt tccagggcgt gcatgccgtc ctcggcggtt accacctcgt
      361 agccgagctg ggtgatgtac ttcgagagga tctcgcggat cgtctgctcg tcgtccacca
      421 ccaggatgcg ctcgcgcgtc gtcgctctgt tgccgttggt cccgtttgct gccacttcgt
      481 ctccttgagg caccgtaaag ctaactttac ggcgaggggg ataattttca aataattttt
      541 atcgtcggtg cgcggttcgc ggcgttgatt cagcgccgct cagtcaaata cgcgtacagc
      601 gccccgccga gctccagtcc ccggtcgatg aagtcgggac cgtagcggcg tccggtgtcg
      661 gtccggaagg tgtcccggat gcgcgtgatg cccgcgatcc ccgcctggta gtgccgcacg
      721 agatcgtcca ccgagatcgc caggctgcgc gccatgctcc agatggtata ggtgaaattg
      781 tccggccccc agcggaactt gtcggcgtcg tagagggcgt cggcgagcag gcgcgcatcg
      841 tcgtcgtcga ttgcgacgcg ttcgcgaaag gcctcgtggt cgcgaatggc gacggcgacc
      901 atggcgatgt cgcgcgcggg aagaatgttc gcaagcaggg gcacgaccgc ctcggccccc
      961 cgggcggagt ggtcgcgcat gttgcgtcgg atgtcgtgca ggtagcccgc gaccagagcg
     1021 agccgcgcga gtccgtcatc atcatcgcct cgctcgcagt agacgatcgc ggcggcgtcg
     1081 tgcgcaacac ggtgcgcatg atagaaatca tggcccagca cgctgccctc ggtccggatg
     1141 ctgtcgcggg cgaaatgcgc ggcgcggtcg ttccgggaca gggcgcgggc gcgatcgagt
     1201 tcgtgcgttt tctcgcggta gaagagcggc cggggggtcg cgcgggagag cgcgatcgcg
     1261 gtccgtttcg cctcggcgat cgtgggcgcg gtcacggtgc ggccgccggg atcagcaatc
     1321 ccccttgctc acgaccttgt tcagggggta ctcgatgatg ccatccgcgc cggcattgat
     1381 gagccgcggg atgagcccgc ggacggtttt ctcgtcgatc accgactcca cggagaacca
     1441 gtcggtgtta tggagccgcg ataccgtggg cgccgtgagg ctgggcacca gcgcgatgac
     1501 cgcgtccagg ttcgccgtgg gcacgttcat cttgatgccc accagatttc ccgcattgag
     1561 cgctcccttc aggagcagcg cgatctggcc gatcttgttc cgcttccacg agtcctgcat
     1621 gctctcgacg ttcgcggcga gcttggtgtt ggtctccagc agggtgtgga tgatcttgag
     1681 tccgtgcgcg cggatggtgg agccggtctc ggtcacctcg acgatggcgt ccacgaggcc
     1741 ctcgaccacc ttggcctcgg tggcgcccca ggagaactcc acctttacgg ggatcccgcg
     1801 ctcggcgaag tagcggctgg tgaaattcac gagctcggtc gagatggtct tgttcgcgca
     1861 gtcctccacc gaggagatgg gggagtgttc ggggaccgcg agcacccagc gggccttgcg
     1921 catgctcacc ttggagtaga tgaggtcgga gatgatctcg accctcgcgt tgttttccaa
     1981 aatccagtcg aggccggtaa ggcccacgtc gagcacgccg ctttcgatgt agcgcgccat
     2041 ctcctgcgcc cggacgagcg agcacgagat ctcgggatcg tcgatggacg ggaaataatt
     2101 tcgcgaggat gacgtgatcc gccatcccgc cttttgaaac agatcgatgg tcgcgttctc
     2161 gaggctcccc ttggggattc ccagcttgag tatcgtttcg ctcatttctt ttctccgtac
     2221 accgtctcgg gatcgaagac ctttgtgccg tcgatccgca ccgtgtcgcc ctccaacacc
     2281 cggtagaagc agctcacgta gccggtgtgg caggccgcct cgccgatctg gttcaccttc
     2341 acgagcacgc agtcgttgtc gcagtcgacg cggatctctt tgacctcctg tatgtttccg
     2401 gaggattcgc ccttcttcca gagcttgttc cgggagcggc tgtagtagtg gacgatgccg
     2461 gtggagcgcg tgaggtccca cgcctccgcg ttcatgaacg ccatcatgag gacctcgccg
     2521 gttcggtggt cctgcgcgat tacgggcacg agcccgccga gcttggaaaa atcgagttct
     2581 atcataatcg ttttgggtcc tttgaatttg gagcaaccat agtagcgggc cgttcgatga
     2641 caagaatttt attttcgcgc acagtcggcg ccgccagcac cgataccatg cgcgggaccg
     2701 tcgcacgggt gtttttatgt tgacatgcgc acgccgacct gctgtattca ccggcacaca
     2761 tcatcaccat caagggagga aatttcacat gggaaaaatt gcagtatccg gggtttcgtt
     2821 attactgttt ctttcgatcg cggtatgcgt gagttcgtct ccggaaacgg agcgcatcaa
     2881 gagatgccgt cccgttttcg acaaatgcat gaaactggcg aacacgacct ccaatcccct
     2941 gtataaggga atggaacggg acgcccgcaa cacggcgatc aaggccgaac gggaaaaatg
     3001 cacgaagtcg tataacgagt gcaaataaga cgccagcggg cgcacggctc tccgccttcg
     3061 cctgcatgag tctccggcgg attgggccga tcgtatcacg gcccggtccg ctggccatct
     3121 tcccctccga cagttcccct ttttccgaca tccactgctg tccgccgcaa tgtcccttga
     3181 ctttttccac ccggtgacgg atgtatttca acgtggcctt catgatttgt aatactatcg
     3241 gagggaatca tgagaaggat gtttcttgtc gccgtgctta tgatgatgac cccggcgttt
     3301 gcggacgacg atgcgaacga acggcttctg ggaaattcgc gaaacggctc cgtgccgggg
     3361 atccgcatgg ccctcaagga cggggcgaag ataaacgagg cgcatgatca tgaatacaac
     3421 acggcgctca tgcttgcggc gggcggcggc catctggaag cggttaaggt gctggtggac
     3481 aacggggccg acgtgaacaa ggtctcggag gggagcagtt attcaacggc gctgatagag
     3541 gcgggcgata cgggctcccc gcaggtgatc gcctatctcc tctccaaggg cgcgaaggtc
     3601 aatgaggcga cctcctacaa acagtccgcg ctcatccggg ccgcatactc gggaaaggcc
     3661 gccaacatca gggcgctcat cgccggcggc gcggacgtaa acatgatgga tgacgccggc
     3721 gcggccatcc acgcggcgtc catgaacggc catggcgacg ccgttacagc gctcatcgcc
     3781 ggaaaggcga atgtgaacct tccggggtcg agccactatg tcacggccct catgctcgcc
     3841 gcggagaagg gacatgcgaa tgtcgtaaaa ttgctgctcg atgcgaaggc ggacgtgaac
     3901 atgaagagcg tggtaaaacg gcaggcgctg gaatacgcgg ccgaaaaggg gcatgcggca
     3961 atcgtgcgca tgctggttgc gaagggggcc gatctgaaca atcagaactc ggagaacggc
     4021 cggacggcgc tctacgcggc ggcctgcaag ggacatgtgg agacggtgcg catcctgctc
     4081 gccgccaagg ccgatcccaa tatcaagtcg acgaacgtcg attacacgcc cctcaagtgc
     4141 gcgagcgaga acttcgagcg catcgacgag gcgcagcatc ggcaactcat ggacctgctc
     4201 cgaaaagccg gggcgcggat ggagtgagga gaatcgctga ttctatagac ggttacgctt
     4261 cgttattgtt ggttttcgcg accggtgaaa ggcgccaaat acttcctgca cgtcgttgag
     4321 aaaggcgaga tactcgtcga aggtcatgcg ttttttcgga cgcattcgaa gcgcggtgcg
     4381 gaaatcctcg ccccgttttt ggtcgagacc atcggcaagc atctcgcgtt tttcttcagg
     4441 tgttagatgc acgtttcatc tcggcaagca gttgggaaaa ctcttcttcc tttccgaaga
     4501 gggcatagta ctccctgatc ctatcaaggt cgaggttttt caagttattt cgcatcaagt
     4561 tgcgtatatc cgccctatcg tggtcggccc gggatggatc attgatactg gattggactt
     4621 tcagtccgat gaggtcctca ggagcgacaa cccgagcagt gaaacgtccg ccaagaaccg
     4681 gcagcggtat gctgcgatcg agcattgcca gtgcgtagcg tcgacgagcg atcaggaaat
     4741 cgacttcctc tgttgtcctg gtgaccccgg atgcatgcaa cgcgagcccg ccaataaggg
     4801 cgaaacggat gtgctccttc tcaaaggatt caaatagaaa accgcacacc gccgcgaagt
     4861 ccatgacggc gctcaggcgt tcttcgatcc gcggaagagc agcacggtgc gaccgatgcg
     4921 gatctcgtcc caatcgtaga gggttttggg gcgcaggagc ttgttgtcgt tgaggaaggt
     4981 gccgttgtcc gacacgaggt cgtagagggt gtaggcgttt ttcacgcgct tgatcttcgc
     5041 gtggttgagc gacaccgcgt cgtccttgat cacgatggcg ttgtcgtcgc cgcggcccag
     5101 cgtcacctcg tcccagaaga tagggaactt tttgcccacc tcggggccgt ccttctccag
     5161 gagccacgcc ttcgcgtaca cgtactccgg atcggtggcc gtgatcacgc ggtcgcccga
     5221 tttccgctcc tgctcgtcga ggtcgatggc cacatggtag tcgcccgcct cggatgattc
     5281 ggcccgcggg gcggcggccc gttccgtctt tcgcgccggg acaaggccgt ttctcctcag
     5341 gaagataacc atgaagacga tcatgccgag gaccaggagg gcgatcagga tcgccagggc
     5401 gatctcgagc gccgtgggca tcgcgatatc gaacaatcgc tttttcacgg tgaagggcgc
     5461 ggtgtcgcgg tcgcggagcg tcccgtactt caggcggacc tcgagcgtgt gcgcccgccc
     5521 gtcggcgggc agcatggatt tatactggac cacgtactgg ttcttgatgg cgcgcaccag
     5581 cgtacggtag atctcgccca cgtccgtcac ctgccgggcg cggtagagcc gtccgccggt
     5641 gagcttggcg atgcgcgaca gctcgcgcac gtaccggtcg ccgtccaccg ccacgaagct
     5701 gatcggtatc tggaggtccc gagcgaattt tatcacatcg cccgcggaca cgctgctccc
     5761 ctcgtccctg ccgtcggtga acacgatcac ggccttccgg ggtccgttcg cgcggctcag
     5821 gaggtccagc gaatcgtaga gggaattgta cagcagggtc tttttcccgt gcgcctccag
     5881 gcggccgatg agcttcgaaa gatcggcccg gttgccggtg aagttcgaca ggagcgcgac
     5941 ctcgctgttg aacttgtaca cggcgatccg gtcgcggtcg gtgctcgcgt ttacgatgtc
     6001 gcgcgcgatc gttttaagct tcgcgaaatt tttcgcatga atgcttttac tggtgtccag
     6061 cgagaagacc aggtagatga ggtcgcgcga cgcctcccgg tccagcatcg tcatgtagtt
     6121 gatccggtac ccgtcctcga tgagcgagag gtgctcatcg tcgaggtcgc tcaggtggcc
     6181 gaccgcgccg tcggtcgccg tgaggtgcac cttcacggcg ggaaaattgg atgagtcgat
     6241 cgaattgatc ttgataaagg ggcccgcggc ggcgcggtgc gccggtgaga cgacgccgaa
     6301 gagcaggcac aggcctgcga gtatgcgtac gattggtgtt cgatggcgtt tctgtctcat
     6361 gaactattcg agcagccgca tggtcgtatg aatccggcgc tgtttctagt atcgggaata
     6421 tggtgcgccg gtttgaatga ttttgtaaaa cagaaaatgt gccggttgta agttatgatg
     6481 tttggtactt gaacttatca aattgaacaa attacgaatt ctatttataa attacgtaca
     6541 atgaattcag gttaggaatc aaatcgtggg agaacgtgaa ccattacttc catcacggtc
     6601 tgatgaaatg gcgagagcca ccagggcaac cggcgcacaa catgcgcctc gtctgcagcg
     6661 tgaatgaatc gtttatccat gcggggacgt tcgtgatatc gtgaaatgaa gtcaatatac
     6721 tgaccgatac gttcgcgccg cagaatgatt attcgcaaat tatatgacaa ccgtccggca
     6781 ataccgaaat tcatttgact ttgtcaatgc catcgactac tatcgaaatg ttgaaatata
     6841 taaaaaggcc catcccctgg cggaataaca cagaaagagc ccggggtgga gattatcaca
     6901 atgatagtca cgactggtaa gcggaatccc cgcagatgga cctctcctga ccatgaatgc
     6961 ttcgcatttg tcttttgcgt gaacggtagt atgagaacgt atccgaggat aatgatttcc
     7021 ctcctctttc atgatcgatc gaagtgcgat ctacaacgag accataagca aaattcgccc
     7081 attgaattca aattggttaa gctcacttaa gaaaaacggc gcagaagtac attatacatt
     7141 aagcactcgg gatatttcat gaagaatcca cgtattatgc aacgaacagt cagtgtattc
     7201 aatatgcaca agaccagcgg acatcccagg ggagaagtca tgaacggaaa gggaatcaga
     7261 aggataacca ttatcgtttt ctgcgcattc attttgaatg gatgcaggtc ttttgatatg
     7321 gtcgaaaatg aaaaacaaaa acatgcgatt gatcagcagg tgccgaacat gacgcttgtc
     7381 gtcaataaac ctagcatcaa ggcgtgcttt gcgaattggc agcattctat agtgacagca
     7441 gaagtaggac ccattggctt ccctcaaatg aaagaagaag ggcttacgtc cgataatcta
     7501 gaatcgtatg tcgagacagt tatcaggaat aattttatta tgcaatcaaa taatcactcg
     7561 aacaagtact tgagcgttat agtgcgaaat atcgattttt cccaaaactg gtggtgggaa
     7621 cccgttacgt ttggtggcat tcttggaatt ttgggaattc cagtttgcag tcaaacctta
     7681 ataattgaaa tggaagcagc ggtcttacag cagaatggaa acatactgaa gatatataag
     7741 acaaaagcag aaaaaaccgc atatggagca ctctactggg ggtatatgtg gattgggcgt
     7801 gcgtcatttt gcatggcgct taataatgca tgtgcagatc ttcgcgagca aatacgccgc
     7861 gatgcggatt acctgaaaag cgtaaaatga ggtatttgat atgaaccata gagcattcat
     7921 tggtgtaata attctcgttg tatctgtttc cttttgtaca ccggtaaaaa gaagttattc
     7981 cggaaatcat gtaattatgt ttgatcaagc agcaccgtca acgttcaaga atagtgatgt
     8041 ggggaatatt atttacatgg tcgacggcag cgaaatacgc ggcgagatcg tgaaccagaa
     8101 tcgcacggcg atcaccgtga agacgaaata cacgacgatg acgatcgaga agaagaatat
     8161 aagggagata aagtataaat aggatgaaaa ccgagaaaat ctttcagtga ttagacgaag
     8221 cccttaattg tcattatttc tggagcatga aatgagttat aggcaacacg tgatacacgc
     8281 gatgctttca atagccatgg catcgttatt caatggctgt gtatcgccaa tgtggcaagc
     8341 catgaaagat ggagatattg aaaaggtgaa aaccttttta gagcaagggg aagatgtgaa
     8401 taaagcggca ttcggcatgt cccccttaac cttcaccgca tattacggca agaccgactt
     8461 ggcgaagctc ctactggaca agggggcaag catagatcga caaagcacga attcactaac
     8521 gcaacagacg gcgacgccgc tcttttgggc tatagatcgc ggtaaccatg atacggctaa
     8581 gtatctcatc agcaggggcg ccagtatcga tgtggcgatt gccgccttcg acgatttgat
     8641 cattaagaat ccttccggga atacgggagc ctggaaacag aacagtgccc tattgcatga
     8701 atggaaaaaa accaggccgg ctggatcaac ggctgggtcc aacgaaaata ccatcttcat
     8761 gaacgacggt agtgaaatcc gtggcgagat cgtgaaccag aaccgaacgt cgatcaccgt
     8821 gaagacgaaa tacacgacaa tgacgatcga gaagaagaat ataaaagaga tcaagtataa
     8881 gtgaatcact ccgcgttgta ttagctatga tgagaaatca ttgagtggga atcaataaat
     8941 attcacaatt cttacggagg gtgacaatga gagtgggtac aataatagct gtaatagtaa
     9001 tttctacaat catcctaatc acgggctgtg tatcattgta caccgcagtt gaggataata
     9061 atgtcaatca agtaaaaatc ttattggaaa aaggtacgaa tccaaatgag agttattctg
     9121 tacatcgagc agcgtggaag ggaaactatg aaatagtaaa gctgttggtg gataaaggcg
     9181 ctgatttaaa tgtcaaatat ttgaataaag gacctttggt atatgcagta tggggtaaaa
     9241 ataatgaaat aattaagctg ttgttggata aaggaaccaa tactgacagt gctatatatc
     9301 aagcggtcaa tgagagtaat atcaatgtaa ttcagatgct attagataag ggtgcagatt
     9361 taaaccgaaa tattgatggc atgcctttaa taagtcaata tgcgtttaag ggagagtata
     9421 agacgtcaaa tgctgaattt attgttacaa gaggggcaaa tgtaattgat gctattgata
     9481 gattggagca ggagatggtg aatcatccaa atgaaacagc aaaatatcaa ttagctatca
     9541 cacgtcttaa taggctaaaa tcattaaatg taaaagcttc aaagtcaaag ccaaaagaat
     9601 ccgaaaacac cattcacttg aacgatggca gtgaaatccg tggagcgata gtcaatcaga
     9661 gccgcacaaa catcaccgtg aagacgaaat acacgacaat gacgatcgaa aaaaagaaca
     9721 tcaaagaaat caaatataaa taaacacacc atttggaggt tgccatgcaa cgcatcacca
     9781 gttcatcatc atggtcaaaa agagcctggt tcatcgcagc aatcgcaagt gtcgtcattg
     9841 gcggaatgtt gatatcgtgc gtaaagaagg accagttttc catgcgtata ctcgcggtgc
     9901 acgggaaggc tacactcgtt tccggggccg tgaccacgga tctcaagccg gacgcaacgg
     9961 tgaacgccgg ggacaccatc gttaccgaga agcagacaac cgtggacctg tacgcgggga
    10021 accagtccgc cgtgcggatt tacgagaata cgaagttcac ggtcaacgcg cgcacggttg
    10081 atcccggcgg gaacatcacc aacgccacta tgggcctgac gggcgggagg atattcgtca
    10141 tcatcaaaaa actggcaatg gacagggcga tatccgtatc gacgccgacc tacgtgatcg
    10201 ccgttcgggg cacggagttc tcggtcaaca catttacgga gaaggaccgg cagctcacca
    10261 agctcgacgt ggtcgagggg aaggtcgaga tcaaatcaac cacgaagccc gagctcgccg
    10321 acacactcgt ggagggcgac accatcaccc tcgcacccga caagaaggag aaggagaaag
    10381 cgaaacttga cgcgccggcc atcgaaaagc tgaagcagga cgccggaacg ctccgagaga
    10441 agacgaccga gtacgagaag aaagccggcg tcgcctcggc gccggatgcc gcgagccaga
    10501 agcccgcagg cgacgacaag gccgcgacgg tcgccgatgc cgggaaaaaa ccggatgaga
    10561 agaaatcggc ggcgcccgcc gacaataaac cgccggtcct caagacggag aagcaaatcc
    10621 gcgagtacta caacaagctc gagcgggtga acatggaaga cggctcggtc ctcatcggtg
    10681 cggtgatcgg gcagagcggg acgcacctgc gcatccacac cgtcaacggc atcatcagcg
    10741 tgcccaagac ggccgtcgtg aatgtggtga tgcagtagtc ggtctctctc accggcggcc
    10801 gagggaatca accgccgtca ccacggccgc cggattcgca tactgcgcca tgtgtccggt
    10861 attccggagg atgatgaggc gcgacgacgg cacggcctca tgaaggcgta ccgaatctgc
    10921 gacggggaca ttgcggtccc tgtcgccgtg aaggatcacc agcggctttc taatcttcga
    10981 atacaagggc gtaagcttcc ccatgcctgc gtcgtaggcc acatcctcct tcgccatcga
    11041 caccagcacc ttcgtttgca gccatatccg caggcgggtt tcgatgtaat cggcgggcag
    11101 ggacgagtcg ttgggatgaa aggcgtaccg gataccctcc gcgaccattt cgcccgcacg
    11161 cactcgcgcg tcgcaggcgg cgatgccgcg cccgatgacg ggaagcgcgg tgatgtgcga
    11221 aaacgccatc gcgccgccgc ggggccggct cgatccggcg atcgacaggt aggccgcgat
    11281 ctccggcggg ttatggacgg cgagcgccag gatcaccccg cctccgtagg agtgccccac
    11341 cgcgatgaca ttgcgcaatg aaagccgtgt gatgagagcg agcgcaagtg tcgcgtttcg
    11401 ctggatgctg aaatcgtcgc tgcgcgcgct gctgaaaccc tggcccggac ggtcgtaggc
    11461 gatcacgcgg tagcgcgtcg ccaggaggcc gaagaccggc tcccattcct cgatggagcc
    11521 ggggaggccg tgcaccagca acacgtcgcg tccggcgccc gcctgatgga atcgaatcgt
    11581 ttcgccttcg atgtcgatgt atctgccggg aaacggcgtg ggtatggcga tatcgtccgt
    11641 ggcggcgatg ccgatgacgg cgaaggcgca aagcgcaaca gcaccgacac agaataatcc
    11701 gatgacatgg tttctgatca tgcgacgcgc ccgaatgcga tatcctcccg cacctcagtc
    11761 cgccgcccgg aagatctcat ccagccgctt cccgtcgcgg tttttcgcgg agcgaagcag
    11821 ggatgcgttg atgccggtgc ggtccgtttc gtagagcagg tcgcaccagc ggacatcgac
    11881 atggttcgcg tagtcggcga tgccggcgat accgctgtag gcgacccgtt tcgacggtcg
    11941 cccgaacagg tcgttctcga cgccggagac gatgcggccg gtccggaata tcaggcggtt
    12001 gtcgtcggta aaaaacgcca cgtggttcat gtggcggcgc tcggcgatgc gcgaaagcga
    12061 ctcgtaggcg ggatcgtccg tgatcaggat gttcccgtcg aggtcggtat agaggtacag
    12121 cccgctcccg cagtcgaaca cctcgtggaa catgcgctgc tcgccgtaca ccagcaggcc
    12181 gatcgacggc gcgatcacgg accactccgc ccgccggtgg cggtacacgc ggcacacctc
    12241 gtcgaattct tctcgggtaa tgcggcgcgg gctgatatag tgcgtggtaa agagctcgcc
    12301 catggcgggc cgggacccct cgcggaagtc ggcgatcgcg atcatgtcgc ggtagcgctt
    12361 cgcccgcggc aggacgccga cgtttccggt gagccggacg gccgtgttcg cgcccgggcg
    12421 catgagggcg agatcgaagc ggtcctgcca ggcaagctcc accatctccc ggcgcttttc
    12481 cacgcgaagc gtcgcgtcgc cgcagggcgc ctggtggacg acgaccgccc gacgcgacgc
    12541 gcatgcgacg gcaagcgcgc ccatcgcgag gacggcgagc aggatgatca gcgtcgcgct
    12601 catgggccct ctcatccggc ggcgcccgcc ggtgcaggaa tcgtgcgaac agcgcttcgc
    12661 gggacgatgc acagcgccga gattattgac gcacgatggt cgcgcgaacc gactcttggc
    12721 acggtgctgg cgcaatcaac acgatgctcg ctttgccttg acattttcgt ccggccatgg
    12781 cacatcacca cgatggaaaa tgcgcttgtt ctgctcgatc tcttcggcac cctggccttc
    12841 gccatatccg gtgcgttcaa ggccgtcaag tacgagctcg atctcctggg gctgatggtg
    12901 ctggccacca tgacggggat cggcggcggc atcgtgcgcg acatcctgtg cggctcgctg
    12961 ccgccggccg cattgagaaa cgagatatac tttttcgtct gcatcgccgg cgggatcgcg
    13021 gtgtactttt tagcgccgcg catcgcgcgc cgctgggaca tcgtcctgtt cgccgacgcc
    13081 gtggggctgg gggtgttcgc gggcatcggg ggcatcaagg gcgtcgatat gggcttcggt
    13141 ttcatgggcg tcatcctcct gggaacgctc accgcgaccg gcggaggcgt tatccgcgac
    13201 atcctggtgt ccgagatccc gatggtgatc cgcagcgatt tctacgccac ggccgtgatc
    13261 atcggctgcg cggcgatggc ggccgtcaat gccgcgggcg cgccccgcgt ggccgtcctg
    13321 ggcgtctgca tcggcgtcac catcctctgc cgtctcctcg ccatgcggtt cgatctttca
    13381 cttcccaagg tgaaatcgct ccccgaatcc cctacgcaaa tgacccgccg acgacggacg
    13441 aaggactaga aggcgctgtg atataaccat tcctccccct aacgaagggg gagatcggaa
    13501 gggggtgtcc cgcagtcctt cacctcgcct ctcaggagtt cgatggcatg ggggatcgcc
    13561 gcctcgatga agccgaggtt ctcgcgcacc gccttggggc tgcccgggag gttgatgatg
    13621 aggctgcgtt ttcggatgcc gctcgcggcg cgcgagagca tggcgtgcgg cgtgatcgcg
    13681 agcgacttcg cgcgcatggc ctccgcgaag ccgggcgcgg ggcggtcgat gaccgcgcat
    13741 gtcgcctcgg gcgtcacgtc gcgcggcgcg aatcccgtgc cgccggtggt gaggatgagc
    13801 tcgacgcccg cgtcggaata gtcgacgagc gcccgtgtga tggcgtcgcg ctcgtccggc
    13861 acgatcgtct cacgcgcgac ggcatacccg tgcgcctcgg cccactcccg gatcgcggga
    13921 ccggacgcgt cctcccgctc gccgcgcgac gaccggtcgc tcacggtgat gacgcccacg
    13981 ctgatcattc tccgcctccg tcgatctcga tgctgttcac ggtgaactcg cggccggaca
    14041 cgatctccat gttggccgag cggcacttct ggcacgagaa gtcgaaggcc tcccggtcga
    14101 gcgcgaactc gaaggcgcag tcgcggcagc ggagcaccgc gggccgccgc tcgatcacga
    14161 gcacggcctt gtcggcgatg gtgtccttgc tcacgaagtc gaagtaattc tgcatccact
    14221 ccgggaccag gtcggagagc tcccccaccg tgaggttgat ctttgtcacc cgcgttgcgt
    14281 tgttccgctt cgcgtgctcg acgacgatgt cgagaatgtt gctcatgacg gacagttcgt
    14341 gcatgccgtc ccccggttcg atatatcgcg cctcacgggg cagtgggcgc gaagatgatg
    14401 aacatgtcaa tcgtatcgtc gtttttgtcg tcgacgatcc cgatccccag gcgcgggtag
    14461 agaaacaggg tgttcgcggg cggcttcgcg atggggtgca tgcccgcatt gccgtaggtg
    14521 aagatgaagt actccacacc gcgcgcgagc gacaccgatt cgggcgttac gcgtcccgtg
    14581 gtgcgcgcga cgatcgccgt cacgcggtcg cgatccatga acaccgcgac gcggcgcgtc
    14641 cggtactcgt atacggcggt aaagaaaagg gtgatcggcg cgggcgtcag gaggacgtca
    14701 cggaagagct cccgccgttc gggaaactgc gccttcgctt cggggacgcc cagccgcccg
    14761 atcacgaccg gggcggcgtc gccgacgacg atgcgctcgg cgcccacgcc cggcacggcg
    14821 agggtctcgt cggccgggac gcgccgtggg aaatcgatcg cgggagacga tacggcgagc
    14881 gcggcgatga gggcgatgcg cgcgagcatg gcggcgcggg cgcgctagat gatgcggatc
    14941 agcaggatga tcatgatgat gatcgcgccg cataggaggg ccacgtacag gaagaggcgg
    15001 ctctcgtcgc tcttctcaga cgacttctcg gcggcctgct ggtccatctc gatcttgacg
    15061 tcctcctcct cgatgatctt gacgagcacg tttttcgcga ccacgccgta gaggacgaag
    15121 ccgccctcgg gcagcgggat cttcgccttg aggcgcgcct tcatgggcag gaactcgtcg
    15181 tcctcggtga tggcgttggt catcttggcc acgctcatcg agatctcgtc gtcgcgcgtc
    15241 aggaccacct tgagggactc gcccgccttg atgtcgttga tgtacttccc cttgatgggc
    15301 gagacgatga tcttgccctc gatcacgtgc tcggcctggg actcgatctc gttcatgcgc
    15361 tggtcgacgc tggccgcggc ctcgcgcgcg tccgccccgc cctcgcgcga cccgggcatc
    15421 tcgatcggga tgccctcggc gtcgatgacc agggagttcg tcgggtcgat cgcgatctgg
    15481 cactggacgc ggtccacctt gaaatatttt ttgaatatgt ccttcaggtc ctcggtgatc
    15541 tcgtccaggt tctcgacctc gagatggtcg tagaggttgt atccctcgat ggccccgatc
    15601 aggtgggtgg tgaagtcgta cgagcccagg gcattgtccc cctcgcggtc gacgtacttt
    15661 ttgtagtcgc cgtagaaggt cttccagccg tcgaagatct ttgatttctc gaacacgccg
    15721 ctcgaggcga ccagcagggc gttgaggttc atgatgtagc ggttcgccgc gttcacgaag
    15781 atgatgaaga tgccgtagac catcgagctc tcgatcgaga gcttcccctt gatcacgcgc
    15841 acgtcgttat actgcccgga ggccatctgc ttggccttgt ccatgtcgcc gttggtgaac
    15901 tgcagcgcgg atttgatgag ccgcgtcttc ttgtcgagcg aatcgaactg cgaggggttc
    15961 tggggttcgg gggccatatc gggcgtccta catcaaattt cggtgaggca tgtttcaata
    16021 atctcgtatt tccgctgcgg cgagagcgtg atgatcttgc gcacggcctg cagcaccagc
    16081 ggcaggtcgt cgatggggaa ctcgagcagg aaacatccgc cctggtcgag ctcgttcacc
    16141 accgtggttt tctttttcgg cagcgagacc cccagcgtgg ggccgaaggt ccgcacgatg
    16201 ttcgcggccg tcaggtactt caccgagagc ttcgtggcgc ccagctcctg gcgccgcacc
    16261 ggagcgtatg cggcgtaacg ctcggccggg aggcgcagcc agcaccgcgg attgtccgta
    16321 atcagaagca ccgcgcgctt ttcctccgtg gaggccagta tccggaagta ggcggtatgc
    16381 ggcttgtacg aggacaggag gccgaacagg ctgtcgtccg tcgttttgat cccttcggat
    16441 atttttttta ctgaaaccat cggcatcgtc ggtcagcggc ccagtgcgcc gctcccgatg
    16501 gttagtgtat cgacatggcg ccagaataca agtaaaaaac ggcggtgccg ccgtcacagc
    16561 ggggtgatga gggccgcgcc gggctcgccc gactggcgga tcagacgcag gccggccagg
    16621 gactccacga tttcggcggt ctcgagcgtg gaccgggaca ggaaatcggc gatctcgctc
    16681 acgtgcgcgg acttgttcac cgagaggtaa ttgatcacgt cccgctcgtt gggcgtgagg
    16741 cggcccagat aatagctgtt gttgggcatg agccggtcgg tcaatacggc gaaggaaccg
    16801 gcggtgtatt gcgcgtgcga cgaaaaaacg gaatcgatgt tcacctgcat gatatcggtg
    16861 atgttggaca gcttcacgta gaagggatgc gtgcggccgc gcgaatcgta cgccagcttc
    16921 acgatgaact cgtcgatgaa cgaggtgtcg atcacctgga tgccctcgaa atcgagcgtg
    16981 agcacctcgt cgggggcgag gccggcgatc ttttcccgca gcagatggta catcgcctcg
    17041 cccatgcgcc gggtgatgag gtccggggag gtgaaggaga gctcgcgggc gatgtcgctg
    17101 atgcgcaggc gcatgcgtgt gctatccgga cagcgatatc gagagctgcg tgccgggaaa
    17161 ggccgctaga tcgtttcgca taaaggtgta gggtttccgc tcgtgcttgc ggttgtacat
    17221 gacggcgatc gaggcgttct ccgaacggat gaagatgctc cccttgagct tttccatcag
    17281 cgtgttcacc tggcagaggc cgtagccgaa gggacgcttg ctggagatgg gcgtcatcag
    17341 cgcctgctcg atgagcttgg cggtcgactc gaactcgatc tccttattgc gcgcgaagct
    17401 ctcgcggatg ccgatgccgg aatcgacgat ggccagccgg acgatgtgct ccgaaccgga
    17461 ggcgtaggtt tgcatcgcgc aatagcccga atccaggctg tgctcgaaga tattctggca
    17521 gatctcggag atcaccgtca cgaaataatc gacgatgctg ttggcgatcc agtacttgag
    17581 gatgtggttg gcgcgcgtcc gaaacagcgc cacaaccgcc gagatggcct gcaggctctc
    17641 gcgctccttg ttggggatgt cggtgatctc gatgacggtc ttgctgaagg agctgcgttt
    17701 gagaaacagc tcctcgtcca acggctcggg cacggtgaac acccctttct tcaggaaatc
    17761 catccgggcg agatactggt gcaccgtgag cggcatgttg atgagggcca gccggtcgcc
    17821 ggtcgcgcgc aggtagttac ggccgataag caggaggttg agcatggaat acggctcgat
    17881 gaacgagatg cgggacaaat caagcgcgtc ggggcgcaca tcggccagcg atacggtccg
    17941 ggacaggacc tcctcgaagg aatacaagtc cttgcagttg ttgggcaaaa caacggcgtt
    18001 tttatatatg gcgttcaccg atccccaccg gaacgttggg agtaaaaaaa agacgtaaaa
    18061 aaatactcaa ctttacgcct tttttccatt gacaaaatac gattaaattt ttggatggtc
    18121 ttaatgtgtc ctcctatttt tttggcgaac ctccaaaaaa cacatttaaa ggggattaac
    18181 catccccttt tttttatatt cgttcgggac gcaggccgct ttcgggcgcg gcggatcagt
    18241 aaaacccctg atactcattg cgcgtgattt tcagcttctt gcgatagtat ttcacgacag
    18301 cgggttttac caagagatag ttggcaatga tatgatccgg ccagccgtat ttgagaagtt
    18361 tgacaatggc ttctttcatg gcgataccct ccttgagata gtgaacctcc gttgggagta
    18421 tagtaaacta cgccccgaaa ttatcaatcc ttttttcgaa tattcaattt tgtgtataat
    18481 agtatttatt tgtacaaatt aaattatgtc acataacggc acggcggggg atgatgatga
    18541 cgatcgatgc gggcggtccc atgtttgagc ggcggccgga cggctcgttc gtcgtgacgc
    18601 cgcaggggcc ggtgcgcatc ggcgccgtgg ggcgcggcgt gggttcggtg gactatacgg
    18661 ccccggccga ggcgatcagg tatgccgaaa aagagatcct tgcctccata accgggatcg
    18721 caccgcggaa catcatcatg ctcaaccagg tccacggaga cgatatcgtg atcatcgacc
    18781 atgcgccggt cgtcgatcga ccctggcatg ccgacgccga cgggctcatc acgcgcgaga
    18841 cggggatatg tctggtgatc cgcaccgccg actgcgtgcc ggtgttcgcg tacgatcccg
    18901 cgagccgcgt cctcggcgcc gcacatgccg gctggcgggg atgccgcgcg gggatcgccg
    18961 cgaagctggt ccgcacgatg cgcgaacgct tcgacgcgcg cccggagcgt atggcgtcgt
    19021 acatattgcc ctcgatcggc ccggattcat actccgttca ggaggatgtc gccggattgt
    19081 ttccacggga cataatcagg aagcgcggtt cgatgtttct taacctctgg acgaacatcg
    19141 agcgcggcat cgtcgagtcg ggggtccccg cggagaacgt gttcaatgcg cgtcggtgta
    19201 cgttgatcga gcgggactct tttttttcgc atcgaaacgg cgatgcgggg cgtaatctaa
    19261 actacgcgta tataatataa aatacgacat ttcacaatcc gtccgttctt tttttcttgc
    19321 gttttttctc atcatcaacc taccctaaat acagataggt cttcgacgca ttgtttcgta
    19381 tcctgtgggc gtatataaat acatgcggtg tgccgtagcg ggggttctcg tgaaaaaaga
    19441 aatcgttcaa ttgtccaatc tggtcgattc atccaaaccg aaaaacatca tcgccgaggt
    19501 taaaaagata ttctcgttcc attacacgga acggcatttc tcgcgcatct cgaaggcggc
    19561 cacgctcgcc atcaacctct tcgaggggcg ctttcccggc tatcaatcgt gcaatacggt
    19621 ctatcacgac ctggagcaca ccatggcggc gctcctggga gtggcgcggc tcctcgacgg
    19681 ctatctcatc cgcggacgca cgctctccga accgctggtc atcaacctgt tcatcgcgac
    19741 actgctgcac gattgcgggt atatccagga gtcatgggac acggagggga cgggcgcgaa
    19801 gcacaccgac agccacgtgg agcgcagcgt ccgcttcgtc gagaagaacc acgaggcctt
    19861 cgggatcgcc gcgggggacg cgcccgtcat cggctcgctc attcgcagca cgggcctctc
    19921 ggtgaacatg gacgacatcc ccttccggtc gccggaggag cgggtcgccg gcgccctcct
    19981 ggggacggcc gatctcctgg ggcagatgtc ggaccgcaag tatctggaga agcttttgtt
    20041 cctgtatcag gagtttcgct aggcgggcat cgccgggttc aacaccgagt tcgacatcat
    20101 ccgcaagacg gtggacttct acgagatgac caagaagcgg atgaacgagt cctatggcgg
    20161 cctgtacaag tacgcgcgct gccatttcca gaagcgaaac gggatcgacg agaacctcta
    20221 cctggtcgcc atcaaccggc acatcgatta cctgcaccgc atcatcgacg acagctcgac
    20281 gaatttcagg aaaaagctga aacgcgccga ctgggtgaac cacgcccaca gcttcgcccg
    20341 ccattaaacc cggccgcgca caggcgggga tcatgccaaa aaaagcccct gcgggttgac
    20401 gttttatcgg aaaagcagta tcttggtgct agagttatgt ctcataatgg aggcgcgatt
    20461 caatgcacca gatcttgttt ctttgcgcga tcatcggggt ggtcgccctg ctcctgcggc
    20521 ttgccgtccg gctcaatcgc aagcaggaag acgatattct cggaaagatt tagccgcccc
    20581 gccggttcgc cgcctacacc gacatcgttt tcccatggag ggtggtgagc ttctcgatgt
    20641 aataccgctt tttccccgcg gggatgttca cgaccacctc ttcgccgacg gtcttgttct
    20701 gcagcgccat gccgatggga gagctgagcg agatcttgcc ctcggaggga ttcacctcgt
    20761 tgggatgcac gaacgtgaac tccacgcgcg cgttcgtttc cttctcgagc acggtgatga
    20821 ttgagccgta gccgatcttg ctctcctcga tggacgacat gctcaggtcg ttgagctggc
    20881 tcatctgatg gctgatctgg tcgatgcgcg ccttaacgaa ggcctgccgc tcgcgcgccg
    20941 cgtcgtactc cgcgttttcc cgcaaatcgc cgtaggcgcg cgcctcggcg atgcgcttgg
    21001 gaagctcaac cttgtattcg acccgaagcg tctcgagctc ttccttgagt tgcttctttt
    21061 tctcgtcgat cgagttttcc atgcgggtac ctcaccggtt ctgatgttag gatgccgagg
    21121 ccgtttttgg cgaaaacgcg gcacgcctta tagtttacgg ctataagcga cagtaggtgc
    21181 ctcgacgatt gcgtgatttc tcgtgcgtcc tgtagtgttt ataacatact aaaggcgcgt
    21241 aaaaacatca attatttttt tgtttttatt ccgtcacgcg atgatcgcgc gcgcggcgag
    21301 caccccgttg atgctcgcct gcatgaggct gcgcgtgatg cccgccccgt cgccggcgac
    21361 gaagagcccc gcgaggccgg tccggaaatc gctcccggtc tcgacgatgt tggagtagaa
    21421 cttcacctcg atgccgtaca gcagcgtctc gtcgctggag aggccgggaa agatgttatt
    21481 gagggcctcg atcatctcct tgatgtccag catgatgcgg tagggcagca ctaggctcag
    21541 gtcgccggga acggcgtcct tgagggtggg ggtgatgttg ttacgcgaca ggcgcgtgac
    21601 ggtggtgcgg cgcccgcgcc ggaaatcgcc ataccgctgc acgatgatct tgttgcccga
    21661 aaggagattt cccagccggg cgatgtactt tccgtattcg atgggctcct tgaacggctg
    21721 ggtgaagtag ttggacacca ggatggcgaa gttcgtgttt tcggtcttga actcgtcgga
    21781 cttgtagctg tgcccgttca ccacggccag tccgtcctcg tagtattccg tcgcgacgat
    21841 gccggccggg ttttggcaga aggtgcgcac gcggtcgtcg aaggtggctg agtagtacac
    21901 gagcttgctc tcgtagacgg cgtcgttgat ctcctgcatg atctcgttgc gcacctcgac
    21961 gcggacgccg atgtcgatgc cgcccacccg cgtgggaatt ccgtgccgcg cgcacagctc
    22021 cttgagccag ctcgcgccct cccgccccac ggcgaggatc acgcggtttg cgcggtactc
    22081 gcgatcggca atcacgccgg ccacggcgcc gttttcgacg atgatgtcct tcaccggatt
    22141 tttgaacagg atggtcacgc cgtcctcgat gagctcgcgc tgcaggttgg cgtagatctc
    22201 cgcgcacttc tcggtgccga tgtggcggat cgggcactca atcatcttca ggttgctttc
    22261 aatggccttc tgccggagct gggtgatcac gtcgtcgctc ccgcgcccgt acacccgggt
    22321 gtcggcgccg ttggcgaggt agacggcgtc cacataggtg atgagcccgg cgagggtgtc
    22381 gcggtccacg tactcgccca gcccgccgcc cacgtccccc gagagcagga gcttcccgtc
    22441 gctaaaggcg cccgcgccgg agaagccggt ggtgatgttg cagggctggc aggaccagca
    22501 ccggtcgtgt tggcgcttgg ggcacgtccg ttcctcgatc cggttgccct tctcgatgag
    22561 gagcacgcgc gttccgggcg agcgcttcgt gagctcgcgc gccgcgaaaa tcccggccgg
    22621 tcctgcgccg acgatgatga tatcgtaggt gtccatatcc tcgagtatgc gatccgttca
    22681 atagcatacg ccggaatgcg gtacgcgtaa agcaaaataa tccgattttc gcggcgtcgc
    22741 gtccggcccg catgacccat acaatttaac gcgcgcggca catttgttgt tgaaaagtat
    22801 ccatgaccgg cgcaggcttt ccctttcgcg cgcgtgctga tggcgtaagc ggatcaacga
    22861 atcgggaagg tgtgcatgaa accacaggtg tttttcattc gcgttgccga gggcgaggac
    22921 gatggcgccg tcgagcggaa gatcggccgg ctgtactcgt ccgcggggtt cgacccgttc
    22981 gtcgcgaagg gcgagaccgt cgccatgaaa acgcacttcg gcgagaaggg caataccacg
    23041 cacatccggg cgaaccacgt ccggcagatc atcaagaaga tcaaggcggc cggcgggaag
    23101 ccctatctca ccgagaccag cgtcctctac aagagcaagc gctcgaacgc gctcgaccac
    23161 atcctcctgg ccatcgagca cggattcggc tacgacaaga tgggcgcgcc catcatcatg
    23221 gccgacggct tcctgggcaa ctgggagcgc gagatcgaga tcaacggcga gtattacacg
    23281 agcgtgtcgg tcgccgggga cgccctcgcc ccggacaagc tcttcatcgt gtcgcacgca
    23341 acggggcacc tggcctccgg gctcggcgcg gcgctcaaga acctgggcat gggactctcg
    23401 agccgcaagg gcaagctcaa ccagcactcg caggccgcgc ccgcggtgaa gccgacggcc
    23461 tgcacgctgt gcgggaagtg catccgctgg tgcccggccg aggccgtcgt cgaacgggac
    23521 ggaaaggcgt tcatcctaac ggaaaagtgc atcgggtgcg gcgagtgcat cgccgtgtgc
    23581 gcgttcgacg cggtgggctt cgagtgggat cagacgagcg aggtgctgca aaaaaagatg
    23641 gtggagcacg cctgcgccat ctgccgggaa aagaaggacc gcattgccta tgtcaccttt
    23701 ctcaccagca tgacccgtga ctgcgattgc atgacgagca agacgaagct catcgacgat
    23761 atcggcatcg tcgcttcgtt cgatccggtc gcgatcgaca cggcgacgct cgatctcacc
    23821 gcgcggttcg cgggcgagaa cctgtcgagg ctttcatatc cgcaatacga tccgctggtg
    23881 caggtgcggc atgccgagaa gctcggcatg ggatcgatgg cctacgagct cgtcgaggtg
    23941 gaataatcgg tcgtgcatta actcgaatag acaacggaag gggatttcga tggaaacaac
    24001 ggttgtcgca aacgacctga aaagcttcag gatgagattt atcctgatga ccgaaggggt
    24061 cgggtatttc gtcatggtgc cgatcctgtt gctgtacgtc tgcaccaacc tcagcctcaa
    24121 cctcgaccag gtggtcctca tgcttctgtg ctcgggagcg gcggtgatcg tgaactcgac
    24181 gttcgccatt gcgcacgacc tgtggacaat ccggcccgtg accggttatt tcgcgaagcg
    24241 cctgcgcggc gatgatatat ccccggagga gcacggacgc gcccagcagc ggttcttttc
    24301 gctcccctac cggcacggca tcaacactgt tatccattgg attaccggcc tactcttggc
    24361 gattgtgccg ttcgtgaacc tcgccgagct cacggcggtg cagaagttca acatgtggat
    24421 ccttttggcc atgatgccgc cgatgggcgg gatggtgtat ttctttctca cggagatgct
    24481 cgtgcagcga ttgttgaacc gcgggatttt cgagaagatc gagatgaagg agttcaccct
    24541 gagcttcagt tttctgggca ggatctcggc gtccatcgcc atcatcctgt cgatgccgat
    24601 cttcgggctc atcggctatt tttccatcat catcgagggc atcaagcccg actaccagtt
    24661 cctttcgata aaactggcgg gcatcattct cctcgtgatc gcgacgtcgc tggtcatctc
    24721 gttcgttctt ttcaaatcga tccgcgacaa gatcgcgatc atcagcgact tcctgaagaa
    24781 gatcggtgcg ggcgatctgt ccgcgaagaa ggggatcatc gcggtggtgg acgagctcac
    24841 gcggatcaac cagacgatct acgtgatgaa gaagaacatc accgacatga tctacgatat
    24901 cgtgcgcatc tccgaccagc tcgacgagtc gaccgcggcc atctcgacgg tgaccgacgg
    24961 cttcaccgag gtgacgcaaa accaggcggc cacgatggag gaggtgaccg cgaccatcga
    25021 gcagatctcg gcgggcatgg accagatcgc caccggcgcg caggaccagg tgaagagcct
    25081 ccgcacgctc atcgaccgga tgggggacct cacctcggtg atccgggaga tggagcggaa
    25141 gatcgccgac gcctccgcgc aggcgggcga gatcacggtg gaggcgcgcc gcggcgagga
    25201 gtcgctggcg aagatgaacg agagcatgga gaacatcggc gagagctcgc ggaagatgac
    25261 cggcatcatc ggcatcatca acgacatctc ggacaagatc aacctgctga gcctgaacgc
    25321 cgccatcgag gcggcgcgcg caggcgaggc cgggcgcggg ttcgccgtgg tcgccgatga
    25381 gatatccaag ctcgccgaca atacggccac gagcgtcaag gagatcgacg cgctcatcaa
    25441 ggcgaccgag cgggagatcg ccagcggcac ggccaccgtg ggcgacgtgg tggagcgcat
    25501 cagccggatc accggcggta tcggcgcgat gaacgaaacg gtgacgagca tcgccgcgtt
    25561 catgaaaacg cagatcgaga ccaacgcgat cgtcgaccgg gaggtggcgc gggtgcgcga
    25621 gaagtccgag gagatcgagg gcgcgaccca ggagcagaag accgcaatcg gggacgtggt
    25681 caattcgatc acgcgcatca acgagctcac ccaggcgatc agcgcgggct cggaggagat
    25741 cgccgcgaac acgaaggaga acgccgacat cgccgacgag ctgaaaaaca aggtgcacca
    25801 gttcacgatc gcgtgagccg gatacggtcc cattgtgtcc gcacgcgcgg gcgcgatggc
    25861 ttccctcttc ctggttgaca gatcgggccg agggtacttt tttcgcgtcg ttcggtaatt
    25921 tttttccttt ttgcaagccg gttttcattc taacatacag cgtctgcaga cgagagacac
    25981 agaaatggcg gcatcgggca gcatgaaagc ctaccggaaa gcgtactgcg agtactatcc
    26041 catcgtgtac agcgttctcc tgtcacgcct gcggaacggc gatgatgccg aagacatatg
    26101 ccaggaggtg ttcatccggt ttctggacaa cttcgattca atcgagagcc atcgtggctg
    26161 gctgctgaag gcaattgaat tcgagatttc gaattactac cggaaaaaga gctcacggaa
    26221 cgacgaggcg gtcgatgtcg ccgaattcgt gaacgatatc aatctcgcct ttgagaacgg
    26281 attccgcgat gcgcgcatcg ttatcaacga ggcaatcgag aacatggaaa actacgactc
    26341 ggaggaggac aggaccctct tcgacctgat cgccgtgtat ggattttcct acaaggaggc
    26401 ggggaaatat ctcggcatga ccaggtggca ggctgaatat cggtatggcc agatcgaggc
    26461 aaggatagtc gattacttaa agaaaatggg catcaaagag gtcttcgatt tgttatgatg
    26521 gaaaagcagg gcccccaggg caacatgaaa gagctgctgc ataaatatcg gctcgatcac
    26581 ccggtcgcgc cggattcgca gaaacggatg atggcgcaca tgggcccgat gtaccgtcga
    26641 atcctgaaac ggaccggacg gcacagtctt atcacgggga tcgtgctgtg gatatacttt
    26701 ctgctccggc gatacggcat cgtcatcaca acgggcaagg cgatcatcgt cacgattgtg
    26761 ctcttggttt tctcgctggg cggcatcctg gcttttttca tgctgcagca tcggcccgac
    26821 agcgcggtga accggaaagg gggcgtcgtt gacattcggg tcgacctgga cagtgccccc
    26881 gtgcaaccgc cccgcccag
//
LOCUS       DAORHF010000024        26890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1110_length_26890_cov_5.220384, whole genome shotgun sequence.
ACCESSION   DAORHF010000024 DAORHF010000000
VERSION     DAORHF010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26890
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1110_length_26890_cov_5.220384"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="PKJ16_03725"
     CDS             complement(<1..439)
                     /locus_tag="PKJ16_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46127.1"
                     /translation="MRDRMNHRLKGVGKRFFLCILLFFSLCNLNQQKLNSQATNTGNN
                     YTWNTINGDWEIKKTTAGKSYLYQSRIRAIPTGYSELINYNSIITVKPVERHTRIGFT
                     LYLTKPGGKPVEALLVFAAKDFRNLHAFKFTGDSKRLTKIALIS"
     gene            complement(500..853)
                     /locus_tag="PKJ16_03730"
     CDS             complement(500..853)
                     /locus_tag="PKJ16_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46128.1"
                     /translation="MKKTIMLILFVFVLSVLFGACNSKKTELQFVNSPDSPGPINDII
                     WANGDAQFSQANGFPVNESTASMEVTELNGRVEAKVLNGSEFVTGNVVIPKTNSSSLS
                     LSEGSANKYTIKVNP"
     gene            1427..1876
                     /locus_tag="PKJ16_03735"
     CDS             1427..1876
                     /locus_tag="PKJ16_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46129.1"
                     /translation="MRKNGSTVRIDGLDGSSPYWLWLDYVSYSRLPGELQGWLDVIID
                     GKPVASIGIHEIAPASAPRRIAIPYECTADGSILVEFRDRSYASGLWGLWGLAVSDSD
                     TVPGEEQFAPRRDGAATGPDVTPRILERKRDPVKAPAGSVRRSGEDK"
     gene            1928..4429
                     /locus_tag="PKJ16_03740"
     CDS             1928..4429
                     /locus_tag="PKJ16_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46130.1"
                     /translation="MRKQSSIIRIAGVAALLALVAGVSYAQTAPAKDAGATLEVKLNA
                     PSGRGAKADGNQNEAVRFSGADEGEYLTMSSGDQIVILRGNIKMEMGAAVISAKVVVY
                     NKNTGDITITDELTFSDGENVIVAQKGVFNVQTKTGVLYGARSTREPLLFQTDKIRIV
                     GEKKYVAEGMGFSTCELERPHYLFRVSKIWIYNDRRVVASNAIYVVGDIPLFYFPVIV
                     HSDEGIGIIMQFGFSNRRGEFLQNTVRYTTEGGFRWKFKLDIYKKMGYYGGIELMKKT
                     DDFDINLYMSGARYKPVDENLEISASLPDENWYKAALDSNMRFLYHEWDTSHMYYNLK
                     FEWMNNWDYERNYDNRREPVTAVDMILFVPRNVFDVRRYLYWNFALGHRGDRHNVMLL
                     FKRTWMWNETLDPKLVDDYYLKGLYAPLYDQLPQFTMRYNGSFRAFEKKDEKTGETSG
                     QTFNWNVNLSNNYYKEYLLGNFFRATNTLLGSGDINTVFPFWTYFAFTPGMKAGFSHK
                     HVEDPNDATRPTSELEARKNSYLFLEPSARLKFGTLQYYLQAGYVYRRTFLESETVEP
                     FVHERLNYLEGGLYLFPLEGIDMSVTTTYDVRPSFPFEDERWRDVVVKNNFFFDFFSY
                     LKSDAASNRKKNLFFAGIGISNSYQYIARDSESGYNTFDIAFMTGNFSIMGIRLFRNI
                     ELGYNWFHDFRHPFRDIMGFRWNVTAELSKWWRLDIGMKSQADSSYLYTQPNTTITPY
                     EDMQKSLYFFNQEKSKGAVFTLRDFHVDLLHDLHCWEIGISYNIERSVRKVGPYNRDR
                     VIYYEQMVFLTFRLKSFSGAGGTGQVYGSKREWEY"
     gene            4528..4821
                     /locus_tag="PKJ16_03745"
     CDS             4528..4821
                     /locus_tag="PKJ16_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46131.1"
                     /translation="MGTEFEKDFDDEVYEDDFYEDGYEGEELVEDDDEGRSADDGDDD
                     LDGDDKRIDVSFACEDCDYRWEDQITKKKDEIDDIDFSDIVCPMCGSVNITQI"
     gene            4818..7628
                     /gene="uvrA"
                     /locus_tag="PKJ16_03750"
                     /pseudo
     CDS             4818..7628
                     /gene="uvrA"
                     /locus_tag="PKJ16_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907899.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
     gene            7739..9778
                     /locus_tag="PKJ16_03755"
     CDS             7739..9778
                     /locus_tag="PKJ16_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46132.1"
                     /translation="MVMSMGAGRRWVMAIACAIAVFASVPAAAQFGDLSRFERAKDAY
                     RKGIVFYNRMQYLAAVEHFRAAVAVYPDYHTAREYLARSYRFAGYTDEALKEWEILAN
                     ISPHNVAVQGKIDALRFRRVGGATAPESAEFVFASQIESNDQTRYRFPRPVDCAVDRD
                     KNVYLSSFSTGKVVKFDANGEGIGTFTTALGSKIYGIDIFDDTVAMTDFQNDVVFLTD
                     RNFRAIKQFGSKGAEAGRFHGPEGVSFDRNGYLYVVDSGNHRVQKFDAAGGFVLAFGQ
                     NGAYEGQLNYPSDVTVIKDRVFVSDSGNKRLACFDDSGNFLRNIGVDGMEGPRGLSEI
                     GGRLVVADEKKGLMMYNPETDVAVWPDLWKNTKGGFSKLFAASFDRDGFLYCVDHHRE
                     TLSVFSPLQSRYSNLDLEITSVDTRKYPIVAVYCNVRNRRGMPVHNLLNKNFRITEDS
                     ARISGVYVDYLKDLDPSVSVVLCVDRSSVAASHHGDLPWAAEFLLRKMRTNDALKVMN
                     FNSDYWTGNDFDWSRRRALQALKERDYRPGKDFGRALYNALNDVVPRITRRAVVVLTD
                     GSVEPESFRRYSADTVIEYARSHYIPISVVAFRQAAPELARIARETGGSVIRVQELDA
                     LRGLYDRVKGQEEYRYVLVYYSFKIADLKGWWSDLRIDVDYLGQKGTEWGGYFVP"
     gene            9768..10418
                     /locus_tag="PKJ16_03760"
     CDS             9768..10418
                     /locus_tag="PKJ16_03760"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019626077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNV46133.1"
                     /translation="MFPDAVDQYANARARMVQSLREISGIYSGAVLRAMSSVPRHRFV
                     SEALRYHAYRDTSLPIGFNQTISKPSTVARMLQALELTGGERVLEIGTGSGYQTALLS
                     RLAGTVVSSEGIDELHKRAREVLLFDLGLANVVLHHTFDFDSLSGEYDAIIVCAGAAV
                     FPGSLLSRLTPNGRLIIPVGGSGGQVLTRYCRTRDDMIIEDSIGRASFVPLVSAVS"
     gene            complement(10428..11366)
                     /locus_tag="PKJ16_03765"
     CDS             complement(10428..11366)
                     /locus_tag="PKJ16_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HNV46134.1"
                     /translation="MPTDFDRFFLNLPPFDRERDFNDFWKKAFQDLKRVPIEPTYDLS
                     SRRSTQRFDLYEVTFRGYNKSRVTGEILYPKGARRAKFAVCLHDYNRPLAYQQAYLDI
                     DLAYFFIRLRGHELIEKRATDQEPSSPGYLVDGILELDNYYAKGVYLDAFRAIDVLRL
                     NSKFDSSEIGMMGKGFGAAAAVFATSFSDRVSALVIDTLAFCCLPVSQNSSTSDATNE
                     INAFIETHKTKKRAVKTNLTYFDAINYADRITCPVLATVGLKDRIAPPECVFSFFNHL
                     QVDKTMEVYPDEGNTAGSENQLVKSIKWMKGFLLEP"
     gene            complement(11377..11793)
                     /locus_tag="PKJ16_03770"
     CDS             complement(11377..11793)
                     /locus_tag="PKJ16_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46135.1"
                     /translation="MYEKPSYENKRLSDRVREWGALGAFIVVSLVISIVVMDVIIFPI
                     SLLSVANKPLFNALVVFGTIGGFAALVALSLARRIIQLRKDGVAAGRIAISIAVAPFK
                     AVGGAIVSLLMSLIVVYAIYLLMSGNYYLLYKLLNR"
     gene            complement(11818..13152)
                     /locus_tag="PKJ16_03775"
     CDS             complement(11818..13152)
                     /locus_tag="PKJ16_03775"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV46136.1"
                     /translation="MQNHPFDSRAVRCVSLIIAALLACGIALPLAAQTAQRPDRAKSA
                     APRDSASARRPEKKPADTPAGVKKPAARDEKRAQPGSAKDAAAPQKTKEALQAEKEQK
                     LKESEEKTVENIEKTLVFGVHKDRADAIGRILKLRRPENKKRLEPKLVEIIASEINAE
                     VRTKAITVAGELEIKAAVPALITAVGDPSEDMRVAAVYALKRIGDPSCVATLSQKLKE
                     QDLSKNANLTEALLNALGEFKTAALAPWAIEGIKNAKTSAANREYLVIFLGRIEARES
                     KDHLLKLLKDEEEDLRIRSYAANSLARMNIKEAAGDINGIIKTIESYSFAKRKKYYNL
                     YIYCLAALARLGDPAAVPRLVDALRSDNAEVRLKAISLIKEIRDKKTMDILKYKMEHD
                     PSPRVQKAAREALAEIEAEAKKQGAQSTAPDRDGPTTPERKPPAENKPAGKK"
     gene            13273..14604
                     /locus_tag="PKJ16_03780"
     CDS             13273..14604
                     /locus_tag="PKJ16_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HNV46137.1"
                     /translation="MKICLAAACAAMLAVALGASVIRDAAAAPGSSYAVVRLDGSVNP
                     ISAGHIVRAIERANAERAQFIVLQMDTPGGMVSSTDDIIKAIYASGIPVVVYAYPKGG
                     EAFSAGAYIMLAAHVAVMAPLTKIGAMHPVALNLFGGGGDGEKNDNVRVMGEKMLNAM
                     LVGARSSAHKRGRNLAWVERAVTKSITATSEEAVGLRIIDFLADDYPDLLKRLDGRTV
                     RLDTGATVVLRTAGSQPIAHEMNWKERFFNFFADPQVVYFLIIIALVGIGIEIKNPGL
                     IVPGVVGILALLFFLLAMRILPVNFFGVILIMLAIVLFVLELKFTSYGLLSVGGIISF
                     VVGSMMLFDSPLPGFSVPLPTIIGVALFVLFLVFVVVRSIVKAHEARVLTGFAGMVGE
                     RGTVLVDFERTGKVLVHGEIWNAESTDPLRKNDAVIVERADSMTLVVKKVQ"
     gene            14629..15387
                     /locus_tag="PKJ16_03785"
     CDS             14629..15387
                     /locus_tag="PKJ16_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HNV46138.1"
                     /translation="MLSLPVTLILLGVMALVSSIKIIKEYERAVVFRLGRLLQAPRGP
                     KGPGIIIIIPLIDKWVRVNMRLIAMDVPPQDIITKDNVSVKVNAVVYFRVVEPNRAIT
                     EVDDYLYATSQLAQTTLRSILGQAELDDLLSNRERINHELQNVLDSQTDPWGIKVTAV
                     EVKHVDLPQEMQRAMAKQAEAERERRAKVIHAEGEEQAAAKIVEAAKQMQQYPVAITL
                     RYLQTLKEVAAEKNSTTLFPIPIDLFEAFMKKLK"
     gene            15398..15763
                     /locus_tag="PKJ16_03790"
     CDS             15398..15763
                     /locus_tag="PKJ16_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46139.1"
                     /translation="MSRAVFTRAGGALLALFIFVSFTACGRITDAVGADDPCPGIRAR
                     FDAVLERASGACVQSNECGCYGAITEKAGCGGVTDKGTADQLGAIAMEFNAAKCAYSR
                     RCAPWLCAPKCVNGRCVRR"
     gene            15834..17003
                     /locus_tag="PKJ16_03795"
     CDS             15834..17003
                     /locus_tag="PKJ16_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV46140.1"
                     /translation="MPHLSRRIGAFTESVIRRMTRVANACGAINLSQGFPDFDPPEEL
                     AAALAKTALVGPHQYAVTWGAPNFRAALARKQSRFMGIPIDPDGNIVVTCGSTEAMMA
                     SMLTVCDPGDRVIVFSPFYENYGADAILSGAEPVFVPLRPPDFTFDPADLRRAFEKRP
                     KAIILCNPANPTGRVFTEGELMAIARLAEEFDAFVITDEVYEHIVYAPHRHVYFASLP
                     GMRARTISCSSLSKTYSITGWRLGYVIADPAIIDGVKKVHDFLTVGAAAPLQEAAIVG
                     LEMPDGYYDRLVESYARKREVFLGALDRAGLRYTRPQGAYYVMVDVSEFDVESDVEFC
                     EWMAREVGVAAVPGSSFFREDVRHLVRFHFAKREETLAAAGERLRSLREKARGRR"
     gene            17000..18850
                     /locus_tag="PKJ16_03800"
     CDS             17000..18850
                     /locus_tag="PKJ16_03800"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV46141.1"
                     /translation="MMALTALRAIIASLIACCTLGMLSCGGGADQLFELRPRTDYRWD
                     DSLAGADGIPALAHDADSAGWRPLDGDGDFRITGERWLWFRVKLPDRSWRDPVVVISE
                     ITQSFDAYYRGARIYRFGSPDELLSQYAAHLIDLPDGAAGGYLYLRMYSRLFKIGVSG
                     PLFEIGERPAVLSRIVRHDADKLLLGFFFLVLGLAAFFYYSSQIDWQLSISLGALSLL
                     AGIYTFCQTDIKQVLHFQNHAFWGAVEIFAFFLIFPALNTFYLRVKRTGALRMVAVCF
                     RWMSIAVPVGAAVAAAFDVNLLARAVLPFEVFCVLNCCTLLALSLADIRRAERGTALF
                     ACGIGAFLLTAIFDVLVDADLLPGVYFYTMHYGLFAFVIVLNRMLARSVGELYRNLKS
                     YADDLRMKNIVVEKAHLELSSLYTELDNTQRDIVYRLCDVIELRSRETGGHVRRVAEY
                     TDILARGYGLPEEDLRVLRIASSMHDIGKLGVPDAILAKPGPLTDEEFRTMQLHTIAG
                     YELLNISQRPIFHSAAIVAREHHEKFDGTGYPDRIAGDTIHVYGRIVAVADVFDSLSA
                     RRSYKEPWRDDDIFAFMDTHRREHFDPDMVSILLSNIDAVVAVKKRFGEG"
     gene            complement(18868..21438)
                     /gene="mutS"
                     /locus_tag="PKJ16_03805"
     CDS             complement(18868..21438)
                     /gene="mutS"
                     /locus_tag="PKJ16_03805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HNV46142.1"
                     /translation="MSDKLTPMMKQYLEIKKQHPNDILFFRMGDFYEMFFDDAATASA
                     VLDIALTARQNDVPMCGIPYHAAESYLARLIKAGYRVAICEQMEAVPSSGTVVRREVV
                     RVVTPGTLVESGLLQSDDSNFLASIVVGEHQVGMAFVDISTGEFFLSAIDRSIELFRG
                     EIVRFSPREVVFRDGSAPDDARFGDYLRSQDIPVNRINDWYYDADYLAGVIAEAYGIA
                     NLKGLGLGGDIEILAAGSIIQYLKDTQRKAFGHLKFPLRIVASNTMVLDDATIANLEL
                     ASNQNDRSRNRTLFSVLNHTRTPMGRRALERMILQPLVARDGIERRLDLVQHFFELYE
                     LNARLRDALAGVHDLERLIARITMGRSFPRDFLMLARSLEAAGAITHILREQPHALCA
                     ALGESIPDLAELARRISAAMDDEPALSPEHGRTIRPGFDQELDRLYKLKTDAKGWIMA
                     YQEDEKKRLGIPTLRVKYNRILGYYIEISKGQTAKAPDDYLRKQTLVGGERYTTETLQ
                     KFETDILSASEKIVAIETAALDTLLRDVSAHRAPLQEAARVIAELDVLCSLASAAVQN
                     RYVRPTFNDEGRTTIADGRHPIVERYYTKEVFIPNDIHLDGERNTILIITGPNMSGKS
                     TYIRMSAIIQLMAQIGSFVPARAADLSIADRIFTRIGASDNIARGESTFLVEMTEAAL
                     ILNNATDRSLIIMDEIGRGTSTFDGLAIAWATVEYLLRYLKAKTLFATHYHELTELGN
                     KDGIANYTVLVKEGLHGVDFLHKVVPGAADKSYGIHVASLAGIPKEITARAAAILERL
                     EKKSKKKTEIPEPAAAEQLEIFNAANHRVIQAIRNIDTDAITPLEALNELNRLKKLIE
                     "
     gene            complement(21441..22607)
                     /locus_tag="PKJ16_03810"
     CDS             complement(21441..22607)
                     /locus_tag="PKJ16_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46143.1"
                     /translation="MRTRAARTRYAIAGAALLGCVLISPHLAHGAHERDDYARVQSAY
                     RSASPDALREAARAYLKNHPRSPHVPDVRLMAALAEAAPGVALRELRAIVDGYPGFPK
                     RDRAQHRICEILTLSADWKRLGVEARFALKVFRDSRLREDFLFFAAQADINTERYDDA
                     ARSTGEIAKTSHHFQTLAGTLLLASYIDRSRSGYSRAYLSSLREIVVGFKDADAVPAA
                     IYLLGRYYESKRDYDRAHSCYADVTGRWPRSPEAAYARKRITALARYKPRTVSYLPDD
                     KTISGSDSIDIHPEMSVTDNDDEDYEAATTFAVSLGPFLNLREALKIKKLVGREFSPV
                     RVVKLRSGFTIYVGRTKSTRTAQSDKIRLAEEYGFNGTVVRVMKNLNRQYIYGE"
     gene            complement(22609..23925)
                     /gene="miaB"
                     /locus_tag="PKJ16_03815"
     CDS             complement(22609..23925)
                     /gene="miaB"
                     /locus_tag="PKJ16_03815"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HNV46144.1"
                     /translation="MRRTFHIETYGCQMNKCDSELMALSMAGAGFDPADGDAADIRIF
                     NTCSVRAHAEDRALANMRAAQAAIRERDGIIVLAGCMAQRIGAGLAGSAAHIVVGPYQ
                     TPDIGRIVRDHLDAGAGAVHLSQEPARLATRINPENIGDADGTPWHKWLTITHGCENF
                     CAYCIVPAVRGPLVSFPSAEIIDYAKRLADHGVIELTLLGQNVNQYGQDSGDISFARL
                     LASISEIDGIRRVNFLTSHPKDFTDDIIETMRDHLAITRAIHLPMQSGSDRILGLMNR
                     RYTVDAYRSLVDRIRAALVDCAISTDLIVGFPGETDAEFRDTLDAVESIGFDDAFMYA
                     YSPREGTAAHALAESITREEKIARLNELIALQRAIGRTRLAVRAGATEVVIVERVSKK
                     SDDEVMGKTALNHPVVLPGGAGDIGAVLRVRIEGVRGNTLYGERVE"
     gene            24536..24760
                     /locus_tag="PKJ16_03820"
     CDS             24536..24760
                     /locus_tag="PKJ16_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46145.1"
                     /translation="MSCYFRHLADILAESGIVVDKENKKDVDRMIHEYLDVEYKNCSD
                     AWRAFKESVRDDDAARKKFIDHLKKYRPRD"
     gene            24806..25993
                     /locus_tag="PKJ16_03825"
     CDS             24806..25993
                     /locus_tag="PKJ16_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV46146.1"
                     /translation="MKTKPIVIVTAGDGPFANAKRALAQLDLAGVRGKRVLVKPNAGR
                     LVGPARGINTHPEALAGVIESLREAGAGDIAIGESPILGVKTLEAFEKSGMAEVARRY
                     GCALIDFDAVPPVEKAIPDARVVTSTKVCAPIYEYDVLLSLPVAKCHMHTGVTLGIKN
                     MKGCLYRREKVRYHQLEYPPGAAWGERTLDSAISDLAMILLPDITVIDGSVGMEGLGP
                     SGGERIESDFAVASFHPLGADVVGCAMMGLDAAEVAHLRLVAERRGWPIDTGAYDVRP
                     ADFRAHHADYRKPPTDISVAYPDVVLYDVESCSACLSTVMLFLKRFKDDMSQYLVDGK
                     LHIAIGKGVRELDLPEGTICVGNCAKRSGTCFFVPGCPPVPTRIYEAITGEEPDKNEP
                     DVS"
     gene            26057..26536
                     /locus_tag="PKJ16_03830"
     CDS             26057..26536
                     /locus_tag="PKJ16_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HNV46147.1"
                     /translation="MLLVAIFAFSVCVFDQLPNRVPAHYNFAGVPDRWTDTSMMGWLS
                     VPLLFAGITALFYGIALTLPWFRKRPQWINMPEESKKRFLALDEEARGAALRGMAAYL
                     YWFPVSSSLMFLWMRYEEYRFIVLREPSFNGAWAIGIIIAGLAITTIAMLRGIKRLV"
     gene            complement(26520..>26890)
                     /locus_tag="PKJ16_03835"
     CDS             complement(26520..>26890)
                     /locus_tag="PKJ16_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNV46148.1"
                     /translation="NIHDVKKAVALRIASKPLDEWMEAFSKVDACVEPVLTLAEAVSR
                     PPVSERGMLVTVRTAAGSGLTQIGNPIRFASGDNCAGWAGTAPGRHTVEILRALGYDD
                     DAIAQLRDCGAIDPAPTPGA"
BASE COUNT         4820 a         8059 c         8651 g         5360 t
ORIGIN      
        1 atgaaatgag ggcgatcttc gtaagccgct tcgagtcgcc ggtgaacttg aaggcgtgaa
       61 ggttccgaaa gtccttcgct gcgaacacca gaagcgcctc gacgggcttt ccgccaggct
      121 tggtgagata gagcgtgaaa ccgatgcgcg tgtggcgctc taccggcttc acggttatga
      181 tcgagttgta attgatgagc tcgctgtagc ccgtggggat cgcccggatg cgactttggt
      241 agaggtagct tttgccggcg gtggtctttt ttatctccca gtcgccgttt atggtattcc
      301 aagtgtaatt attgcctgtg ttagtggctt gtgagtttaa tttctgttgg ttgaggttgc
      361 agagagaaaa aaacagaaga atgcaaagaa agaaacgctt gcccacccct ttgagccgat
      421 gattcattcg gtctctcatt tccttttcgt gatattgaaa acccttatgg gctctcaata
      481 tcacattggg gcaattattc tatggattta ctttaattgt atatttgttt gccgacccct
      541 cgctcagaga aagtgaagac gaattagttt tcggaataac cacattgcca gttacaaatt
      601 cacttccgtt taataccttt gcctcgaccc tcccgttcaa ctcagtcact tccatcgatg
      661 ctgtactctc gttaacagga aatccatttg cttggctgaa ttgcgcatct ccattcgccc
      721 aaatgatatc gttaataggc cctggtgagt ctggtgaatt cacaaactgc aattccgtct
      781 tcttgctgtt gcacgcgcca aagagcacag ataacacgaa tacgaagagt atcagcatta
      841 tcgttttttt catgatagtc cccctcacaa tcgaataaaa tcgcagggat aaaccgctgc
      901 cgcatgatcc atcgtgatgc tcacggtttc tcatacgcct caaataaaat caaccacaat
      961 aataatttta ttgtaaataa atctaccacc cggcccaagc gctaccgaat tatatataag
     1021 atagggcgga gtatgccggc agtcaagttt ttgggagcac caggttggct atttcgcgat
     1081 ttgcggtcct ttggctcatt attttcggcg ggtagggggt ttcctgcttg catttgtgtt
     1141 tcatagcgga gacgtggagt tatcgatgcg gggaaaaatg aacagtagtc gtattatcgt
     1201 cattattatt gctatcagtg tttgcgtgtt gatcgccatc aggagcggcg gtgccgcgga
     1261 tcggcgtgcg ccaatagcca ttgtctttga cggcacgaat gatgagcgcg ccatcaagaa
     1321 ctggagcgag cccattgcct accgcgagat cgctcgacgg gcgcgggtga taggcgccga
     1381 ggttctttca ccgggtggcg cgggatcccc gcgaatggcg cgcttcgtga gaaaaaacgg
     1441 gtcgacggtg cgcatcgacg ggctcgacgg cagctccccc tactggctct ggctcgatta
     1501 tgtctcctac agccgcttgc cgggcgagct ccagggatgg ctcgatgtta tcatcgacgg
     1561 gaaacccgtc gcgtccatcg gcatccatga gatcgcgccg gcctccgcgc cccggcggat
     1621 cgcgatcccc tatgaatgca cggccgacgg gtccattctc gtcgagtttc gggaccgttc
     1681 ctatgcgagt ggtctatggg ggctctgggg ccttgcggtt tctgattccg atacggttcc
     1741 gggcgaggag caattcgctc cgcggagaga cggcgccgca accgggcccg atgttacgcc
     1801 gcgcatattg gagcgaaaac gcgatccggt gaaggcgcct gcgggatccg tgcgccgttc
     1861 tggtgaggac aagtaggcgg tcgggccgcg agcgcgctcg ccgattccac cgcatcgtga
     1921 ttaccgcatg aggaaacaat cgtcaatcat tcgaatagcc ggcgtggccg cgcttctggc
     1981 gctcgtcgcg ggcgtgtcgt atgcccagac ggcaccggcc aaggatgccg gcgccacgct
     2041 cgaggttaag ctgaacgcgc cctcgggacg cggggcgaag gcggatggca accagaacga
     2101 ggcggttcga ttctcgggcg cagatgaggg cgaatacctc acgatgtcaa gcggcgacca
     2161 gatcgtcatc ctgcgcggca acatcaagat ggagatgggc gccgccgtca tttcggcgaa
     2221 ggtcgtcgtg tataacaaga ataccggcga catcaccatc accgacgagc tgaccttcag
     2281 cgatggcgag aacgtgatcg tcgcgcagaa gggcgtgttc aacgtccaga cgaaaacggg
     2341 ggtgctctac ggggcgcgct cgacgcgcga gccgcttctg ttccagaccg acaagatccg
     2401 catcgtgggc gagaagaaat acgttgccga ggggatgggc ttctccacct gcgagctcga
     2461 gcggccgcac tacctgttcc gggtttccaa gatatggatc tacaatgacc ggcgcgtggt
     2521 ggccagcaat gccatctacg tggtgggcga catcccgctc ttctacttcc cggtgatcgt
     2581 ccatagcgac gagggcatcg gcattatcat gcaattcggg tttagcaacc ggcggggcga
     2641 gttcctgcag aacacggtgc gctacaccac ggaggggggc ttccgctgga agttcaagct
     2701 cgacatctac aagaagatgg gctattacgg cggcatcgag ctcatgaaga aaaccgacga
     2761 tttcgacatc aacctctata tgtccggcgc ccgctataag cccgtcgacg agaacctgga
     2821 gatatccgcc tccctgcccg acgagaactg gtataaggcc gcgctggatt cgaacatgcg
     2881 cttcttgtat cacgaatggg acacgtcgca catgtattac aatctcaagt tcgagtggat
     2941 gaacaattgg gattacgagc gaaactacga caaccggcgc gagcccgtga ccgcggtgga
     3001 catgatcctg tttgtcccgc gaaatgtgtt cgacgttcga cgatacctgt actggaactt
     3061 cgccctcggc caccgcggcg accggcacaa tgtcatgctc ctcttcaagc gcacctggat
     3121 gtggaacgag acgttggacc cgaagctggt cgacgattat tacctgaagg gcctgtatgc
     3181 gccgctctac gatcagctgc cgcagttcac catgcgctac aacggcagct tccgggcctt
     3241 cgagaaaaag gacgagaaga ccggcgagac gagcggccag accttcaact ggaacgtgaa
     3301 cctgtcgaac aattactaca aggagtacct gctcggcaat ttcttccgcg ccacaaacac
     3361 cctcctgggc tcgggcgaca tcaacacggt cttccccttc tggacctatt tcgcgttcac
     3421 cccgggcatg aaggccggct tcagccacaa gcatgtggag gacccgaacg acgcgacgcg
     3481 ccccaccagt gaactcgagg cgcgaaagaa ctcctatctg ttcctggagc cgtcggcgag
     3541 gctcaagttc ggcacgctgc agtactatct gcaggcggga tatgtctacc ggcgcacgtt
     3601 tctggagagc gagacggttg agccgttcgt ccacgagcgg ctcaactatc tcgagggggg
     3661 gctgtatctg tttccgctcg aggggatcga catgtcggtg accaccacct acgatgtgcg
     3721 cccgagcttc cccttcgagg acgagcgctg gcgggacgtc gtcgtgaaga acaatttttt
     3781 cttcgatttt ttcagctacc tgaaaagcga tgcggcgagc aaccgcaaga agaacctgtt
     3841 cttcgcgggg atcgggattt cgaactcgta tcagtacatc gcccgcgaca gcgaatcggg
     3901 ctacaatacc ttcgatatcg ccttcatgac gggaaacttc tccatcatgg ggatccggct
     3961 gttccggaac atcgagctcg gctacaactg gttccacgac ttccgccacc cctttcgcga
     4021 catcatgggg ttccgctgga acgtgaccgc cgaactgtcg aagtggtggc ggctcgatat
     4081 cgggatgaag tcgcaggcgg acagttcgta tctctacacc cagccgaata cgacgatcac
     4141 accctacgag gacatgcaaa agtcgctgta cttcttcaat caggagaagt ccaagggcgc
     4201 cgtgttcacc ctgcgcgatt tccatgtgga cttgctgcac gatcttcact gctgggagat
     4261 cggcatctcg tataatatcg aacgctcggt gcgcaaggtg ggcccgtaca accgcgaccg
     4321 cgtcatctac tacgagcaga tggtgttcct caccttccgc ctcaagtcct tctccggcgc
     4381 gggcggaacg gggcaggtgt acggcagcaa gcgcgagtgg gagtactaga aaaaaatttg
     4441 acaaaataaa tcgcgaactt ttggttgata tccgatgccg cgcgacgtat cttagccgtg
     4501 gaatacacta catgccaggg agcagtgatg ggaaccgaat tcgaaaagga ctttgacgac
     4561 gaagtgtacg aagacgattt ctacgaagac ggctatgagg gcgaagagct cgtggaggac
     4621 gacgacgagg ggcgctccgc cgacgatggt gacgacgatc tcgacggcga cgacaagcgc
     4681 attgacgtga gcttcgcctg cgaggactgc gactaccgct gggaagacca gatcaccaag
     4741 aaaaaagacg agatcgacga tatagacttc tccgacatcg tgtgcccgat gtgcggatcg
     4801 gtcaatatca cgcagatatg agccagccga cccttcgcat caggggagcg cgcgaacaca
     4861 acctcaagaa catctcactc gacatccccc gggatcggct ggtggtgatc accggcctct
     4921 ccggctcggg gaaatcctcg ctcgccttcg acacgatcta cgcggagggc cagcggcgct
     4981 atgtggagtc gctgtcggcc tatgcgcgcc agttcctcgg cctcatggaa aagcccgatg
     5041 tcgatttcat cgacgggctc tcgccggcca tctccatcga gcagaagacg acgcaccgga
     5101 acccgcgatc cacggtgggc accgtcaccg aggtgtacga ctatctccgc ctgctcttcg
     5161 cgcgggtggg gacgccgcac tgccaccgct gcggaaagat gatatcgtcg caatcggtgg
     5221 accagatcgt ggagtcgctg ctcacgcggc ccgcgggaac gaagctgcag gtgctcgcgc
     5281 ccatcgtgcg cgggcggaag ggcgagcacg ccgaccgtct cgagaaggcg cgacgggacg
     5341 gcttcgtgcg cgtccgcatc gacgggcagg tccgctcgct cgatgaggac atcgccatcg
     5401 acaagaagaa gaaccatacc gtcgagatca tcatcgaccg ccttgccgtg aaggaggggg
     5461 tgcgcccgcg gctggccgag tccgtcgaga ccgcgatgga tatcgccgac gggctcgtcg
     5521 gggttttggt gaacgacgag gagcatctct tctcgacgcg cctctcctgt cccgactgcg
     5581 gcatctccat gccggagctt tcgccgcgga tcttctcgtt taacagcccc ttcggcgcct
     5641 gcgagaaatg cagcgggctg ggcttcatca tgcgcttcga cccggagcgg atcgtctcgg
     5701 acccgaagcg ctcgctctac gatggggtcc tggaggtgtg gggcaagacc accagctact
     5761 ggtatcgcga gcagatcgag gccctggagc gcaatttcaa ctttgacgcg aacctcccct
     5821 gggaaaagat cccgcgaaag gtaaaggata tcatcctgta cggctccggc gggaagaaac
     5881 tctctttcga tgtgaagcgg acgaacgcgg agtacaagtt ctcccggagc ttcgaggggg
     5941 tgatccccaa cctggagcgc cggtaccgcg agacgaaatc ggacgagatg cgcgaatgga
     6001 tggagggcta catgtccaac caggtgtgcg acgcctgcga gggtcgccgc ctgcgccccg
     6061 agagcctcgc cgtgcgcgtg gcgggcaggg gcatcgacga ggtgaccggg ctttcgatcg
     6121 gggcggccgc ggaattcttc gaatccatcg cgctttcgga cgttgagcga aagatcgccg
     6181 cgcagatact gaaagaaata cgggcccggt tgcgctttct cacggacgtg gggatcgact
     6241 acctcacgct ctcgcgcgcc gcggggacgc tctcgggcgg cgagtcccag cgcattcgcc
     6301 tggcgacgca gatcggctcc agcctcaccg gcgtgctcta cattcttgac gaaccgagca
     6361 tcgggctgca ccagcgcgac aatgcgcggc tgctggagac gctgaagaac ctgcgcgaca
     6421 tcgggaacac ggtgatcgtg gtggagcacg atgagagcac catgcgcgag gccgatttcg
     6481 tcgtggacct ggggcccggc gcgggtgtcc acggcgggca tgtcgtggcc gcgggcacgc
     6541 ccgagtagat catggccgac cggaagtcca tcaccggaaa gtacctgagc ggcgagcgag
     6601 ccattccggt gcccccccga cggcgggagc ctgcgggttt ccttgagctt cgcggggcgc
     6661 gcgagaacaa ccttaagggg atcaccgcac gctttcccct gggggtgctc tcctgcgtga
     6721 cgggcgtgtc cggatcgggg aaatcgaccc tgctcatcga cattctctac aaggcgctct
     6781 cggcgctggt catgggatcg aaggagtatc ccgggcgctt cgacgagatc gtcgggacgg
     6841 agctcatcga caaggtgatc gacatcgacc agtcgccgat cgggaggacg ccgcgctcga
     6901 acccggccac gtacaccggc gtgttcacgc ccatccgcga tctcttcgcg aacctgccgg
     6961 aatcgaaggt gcgcggctac aaggccgggc ggttttcgtt caacgtgtcc ggcggccgct
     7021 gcgaatcgtg cgagggcgac ggcatcaaaa agatcgagat gcacttcctg cccgatgtct
     7081 atatcccgtg cgaggtgtgc aagggacggc ggtacaacca cgaaacgctc gaggtgcgtt
     7141 tccgggggaa gaacatcttc gaggtgctgg acatgacggt ggaggaggcg ctcgggtttt
     7201 tcgagaacat accctccgtg cgcggcaagc tcgcgaccat cggccaggtg ggcctcgggt
     7261 acatcaagct ggggcagccg gccaccacgc tctcgggcgg cgaggcgcag cgcgtgaagc
     7321 tcgccaccga gctctcgaag cgcgcgaccg ggaggacgct ctatatcctg gacgagccca
     7381 ccacggggct ccacttcgac gacgtgcaca agctgctggg cgtgctcgcc tcgctggtgg
     7441 agaagggcaa caccgtgctc atcatcgagc acaacctgga cgtgattaag accgccgact
     7501 ggatcatcga cctcgggccc gaggggggag acggcggcgg ggagatcgtg gccgagggta
     7561 cgcccgaggc cgtggcgaag gtccgccggt cgcacactgg cgcgttcctc aagcccctgc
     7621 tgaaatagcg ccgcccgccg ccgcatcatc cgcgcgccgt ttcccgctca tgattttttt
     7681 agtgcttgac gataatttcc aatatgacat aattcttgat gtggacaggg agccgtacat
     7741 ggtgatgtcg atgggtgccg ggcggcgctg ggtgatggcg attgcgtgtg cgatcgccgt
     7801 cttcgcgagc gtccccgcgg cggcgcagtt cggggacctc tcgcggttcg agcgggcgaa
     7861 ggatgcgtat cgcaagggga tcgtcttcta taaccggatg cagtacctgg cggcggtgga
     7921 gcatttccga gctgcggtgg cggtgtatcc cgactatcac accgcgcgcg agtacctggc
     7981 gcgctcctac cgcttcgcgg gctacaccga cgaggcgctc aaggagtggg agatcctcgc
     8041 gaacatctcc ccgcacaacg tggccgtgca ggggaaaatc gatgcgctgc gcttccgccg
     8101 cgtggggggc gcgacggcgc cggaatcggc cgaattcgtg ttcgcaagcc agatcgaatc
     8161 gaacgaccag acgcgctatc gctttccgcg tccggtcgat tgcgccgtcg accgcgacaa
     8221 gaacgtgtac ctgtcgtcct ttagcacggg caaggtggtc aagttcgacg cgaacggcga
     8281 gggcatcggg accttcacca ccgcgctggg cagcaagatc tacggcatcg acatcttcga
     8341 tgatacggtc gcgatgacgg atttccaaaa cgacgttgtg ttcctgaccg accggaattt
     8401 tcgcgccata aaacagttcg gctccaaggg cgccgaggcg ggccggtttc acgggcccga
     8461 gggcgtgagc ttcgaccgca acggctatct gtatgtggtc gattccggga accatcgcgt
     8521 ccagaagttc gacgccgcgg gcggctttgt cctcgccttt ggccagaacg gcgcctacga
     8581 gggacagctg aactatccga gcgacgtgac ggtgatcaag gaccgcgtct tcgtgagcga
     8641 cagcggcaac aagcggcttg cctgtttcga cgattccggg aacttcctcc ggaacatcgg
     8701 cgtggacggg atggagggcc cgcgcgggct cagcgagatc ggcggccggt tggtcgtcgc
     8761 ggacgagaag aagggactca tgatgtacaa tcccgaaacg gacgttgcgg tctggcccga
     8821 tctgtggaaa aacaccaagg gaggattctc gaaacttttt gccgcgagct tcgaccgcga
     8881 cggcttcctc tattgcgtcg atcatcatcg cgagacgctc tcggtgtttt ccccgctgca
     8941 gagccggtac tcgaacctgg acctcgagat cacgtcggtg gacacgcgga agtatccgat
     9001 cgtggccgtc tactgcaatg tccgcaaccg tcgggggatg ccggtgcaca atctgctgaa
     9061 caagaacttc cggatcaccg aggacagcgc ccgcatctcg ggagtgtatg tcgattatct
     9121 caaggacctg gacccgtcgg tgtcggttgt cctgtgcgtt gaccgctcgt ctgtcgcggc
     9181 gtcgcatcac ggcgacctgc cctgggcggc ggaattcctg ttgcggaaaa tgcgcaccaa
     9241 cgacgcgctc aaggtgatga acttcaacag cgattactgg accggaaacg atttcgactg
     9301 gagccggcgg cgcgcgctgc aggcgctcaa ggagcgcgat taccggcccg ggaaggattt
     9361 cgggcgcgcg ctgtacaatg cgctcaacga cgtggtgccg cggatcacgc ggcgcgcagt
     9421 ggtggtgctc accgacgggt ccgtcgagcc ggaatccttc cggcgctatt ccgccgacac
     9481 cgtgatcgaa tacgcccgca gccattatat cccgatctcc gttgtcgcgt tccggcaggc
     9541 ggcgccggag ctggcgcgca tcgcccgcga gaccggcggg agcgtgatca gggtgcagga
     9601 gctggacgcg ctgcgcggac tgtacgatcg cgtgaagggc caggaggagt accgctacgt
     9661 gctggtctat tacagcttta agatcgccga cctgaagggc tggtggtccg atcttcgcat
     9721 cgatgtcgat tatctcggcc agaaggggac cgagtgggga ggatactttg ttccctgacg
     9781 cggtcgatca gtacgccaat gctcgggcgc ggatggtgca gtccctccgg gagatttccg
     9841 ggatttactc gggcgcggtc ctgcgggcga tgtcgtccgt tccgcgccac cgcttcgtct
     9901 cggaggcgtt gcgctatcac gcctatcggg acacctcgct gccgatcggg tttaaccaga
     9961 ccatatcgaa gccgtccacc gtggcgcgca tgctgcaggc gctcgaattg accgggggtg
    10021 agcgcgtgct ggagatcggt acggggtccg gctatcagac ggcgctcttg tcacgcctcg
    10081 cggggaccgt cgtgagctcc gaggggatcg acgagctgca caagcgcgcc cgcgaagtgc
    10141 tcctgttcga tctggggcta gcgaacgtcg ttttgcacca caccttcgac ttcgattcgc
    10201 tttcgggcga gtacgacgcg atcatcgtgt gcgccggggc ggccgtgttt cccggatcgc
    10261 tcctgtcgcg gctcacgccg aacggccggt tgatcattcc cgtcggcggg agcggggggc
    10321 aggtgctcac gcgctattgc cgcacgcgcg acgacatgat tatcgaggat tcgatcggcc
    10381 gggcgtcgtt cgttccgctg gtgagcgcgg tatcctgagc cgcgccgtca gggctccagc
    10441 agaaatccct tcatccactt gatcgatttg accagctggt tttccgagcc cgcggtgttt
    10501 ccctcgtcgg gatacacctc catggtcttg tccacctgga gatggttgaa aaacgagaat
    10561 acgcattcgg gaggcgcgat gcggtccttg aggcccaccg tcgcgagcac cggacaggtg
    10621 atgcggtccg cgtaattgat cgcatcgaaa taggtgaggt tcgttttaac ggcgcgtttt
    10681 ttcgttttat gggtttcgat gaaggcgttg atctcgttgg tggcgtcgct cgtcgagctg
    10741 ttctggctta ccggcaggca gcagaaggcg agggtgtcga tcaccagcgc cgagacgcgg
    10801 tccgagaacg acgtggcgaa caccgcggcg gcggcgccaa agcccttgcc catcataccg
    10861 atctcgctgc tgtcgaattt tgaattgagt cgcagcacgt cgatggctcg aaacgcgtcg
    10921 aggtacaccc ctttcgcata gtagttgtcc agctcgagga tcccgtcgac gagatagccc
    10981 ggcgaggacg gttcctggtc cgtggcgcgc ttttcgatga gctcgtgtcc gcgtaggcgg
    11041 atgaagaagt acgccaggtc gatatccaga taggcctgct gataggccag cggtcgattg
    11101 tagtcgtgca ggcacacggc gaacttcgca cggcgcgcac ctttcgggta gaggatctcg
    11161 ccggtcaccc gcgacttgtt gtagccccgg aaggtgacct cgtagaggtc gaaccgctgg
    11221 gtggaccggc ggctgctcag gtcgtaggtc ggttcgatgg gaacgcgctt gaggtcctga
    11281 aacgcctttt tccagaagtc gttgaaatcg cgctcccggt cgaagggcgg caggttcagg
    11341 aagaagcggt cgaaatccgt gggcatgtgc cgtccgctat cggttcagga gcttgtacag
    11401 gagatagtag ttcccgctca tgagcaggta gatcgcgtag acgacgatca gcgacatcag
    11461 cagggagacg atggcgccac cgacggcctt gaatggggcg acggcgatgg agatcgcgat
    11521 cctgcccgcg gccacgccgt ccttccggag ttggatgatc cgtcgcgcga gcgagagggc
    11581 gaccaaggcc gcgaacccgc cgatggtccc gaagaccacc agcgcgttga acagcggctt
    11641 gttcgcgacc gagagcagcg atatggggaa gatgatgacg tccatgacga cgatggatat
    11701 cacgagcgat acgacgatga aggccccgag cgccccccac tcgcggaccc ggtctgagag
    11761 tcgtttgttc tcgtagctcg gcttttcgta catggcgaag tgtccgtgcg cggcgtatta
    11821 ttttttcccg gccggtttgt tctcggccgg gggcttccgt tcgggtgtcg tcggtccgtc
    11881 gcggtcgggg gcggtcgatt gggcgccctg ctttttcgcc tccgcctcga tctcggcgag
    11941 cgcctcgcgc gccgccttct gcacccgcgg actaggatca tgctccatct tgtattttaa
    12001 aatgtccata gtttttttgt cccgtatctc cttgatgagg ctgatcgcct tgaggcgcac
    12061 ctcggcgttg tcgctgcgca gcgcgtccac gaggcggggc acggcggcgg ggtcgcccag
    12121 gcgggccagc gcggcgaggc agtagatgta caggttgtag tacttcttcc gcttcgcgaa
    12181 ggaatacgat tcgatcgttt tgatgatgcc gttgatgtcg ccggcggcct ccttgatgtt
    12241 catgcgcgcc agggaattgg cggcatagga gcggatgcgc aggtcctcct cctcgtcctt
    12301 gagcagcttc aggaggtggt ccttcgattc ccgggcctcg atgcgtccga ggaagatgac
    12361 gaggtattcc cggttcgcgg ccgaagtctt cgcgttttta atgccctcga tcgcccaggg
    12421 agcgagcgcg gcggtcttga actcgcccag ggcgttcagg agcgcctcgg tgaggttggc
    12481 gttcttgctc aggtcctgct ccttgagctt ctggctgagc gtggcgacgc aggagggatc
    12541 gccgatccgc ttgagcgcat agacggccgc cacgcgcatg tcctccgacg gatcgcccac
    12601 ggccgtgatg agggcgggga cggcggcctt gatctcaagc tcccccgcga cggtgatggc
    12661 cttggtgcgc acctccgcgt ttatctcgga ggcgatgatc tcgacgagct tcggctccag
    12721 gcgcttcttg ttttcgggac ggcgcagctt gaggatcctg ccgatcgcgt cggcgcggtc
    12781 cttgtgtacg ccgaagacca gcgtcttttc gatgttctcg acggtcttct cctcgctttc
    12841 cttgagcttc tgctccttct cggcctggag cgcctccttc gttttttggg gagcggctgc
    12901 gtccttcgct gatccgggct gcgcgcgttt ctcgtcgcgg gccgccggtt tttttacgcc
    12961 ggccggcgtg tccgcgggtt tcttctccgg tcgcctggct gatgcgctgt cgcgtggggc
    13021 ggccgatttc gcgcggtcgg gccgctgcgc ggtttgcgcc gcaagcggga gcgcgatgcc
    13081 gcacgcgaga agcgcggcga tgatgagcga aacgcaccgg actgcgcgcg aatcgaacgg
    13141 atggttttgc atgagtgccg tccccgtggt taataacttg acagacgcgt tggaatatgc
    13201 tataccgtac ccggtcgtgg tgacaatcaa attttttccg ggaatcggca aatggcacag
    13261 agggcccgtg ttgtgaagat atgtctcgca gcggcgtgtg ccgcgatgct ggcggtcgcg
    13321 cttggcgcgt cggtgatacg cgatgcggcg gcggcgcccg gatcgtccta cgcggtggtc
    13381 cggctcgacg gctcggtcaa tcccatcagc gccggccaca tcgttcgcgc gatcgagcgc
    13441 gcgaacgccg agcgcgcgca gttcatcgtg ctgcagatgg acacgcccgg cggcatggtt
    13501 tcctccacgg acgacatcat caaggccata tacgcctccg gcataccggt ggtggtgtac
    13561 gcctatccca agggcgggga ggccttttcc gcgggcgcgt acatcatgct ggccgcgcat
    13621 gtcgcggtca tggcgccgct gacgaaaatc ggcgcgatgc atccggtcgc cctcaatctc
    13681 ttcggcggtg gcggcgacgg cgagaagaac gacaatgtgc gcgtcatggg cgaaaagatg
    13741 ctcaacgcca tgctcgtcgg tgcgcgcagc tccgcgcaca agcgcggacg aaacctcgcc
    13801 tgggttgagc gcgccgtcac gaaatcgatc accgcgacca gcgaggaggc cgtcgggttg
    13861 cgcatcatcg attttctggc cgacgactat cccgacctcc tcaagaggct cgacggcagg
    13921 accgtgcgcc tcgacaccgg cgccaccgtc gtcctccgca cggccgggtc gcaacccata
    13981 gcgcacgaga tgaattggaa ggagcgcttc ttcaacttct tcgccgatcc gcaggtcgtg
    14041 tacttcctga tcatcatcgc cctggtgggc atcgggatcg agatcaagaa cccggggctc
    14101 atcgtacccg gggtcgtggg cattctggcg ctcctcttct ttttgctggc catgcggatt
    14161 ctgccggtca actttttcgg cgtcatcctt atcatgctcg ccatcgtgct gttcgtgctc
    14221 gagctcaaat tcaccagcta cggcctattg tccgtgggtg gcatcatatc gtttgtggtg
    14281 ggttccatga tgctgttcga ttcgcccctg ccggggtttt ccgtgccgtt gccgaccatc
    14341 atcggcgtgg cgctgttcgt gctgttcctc gtgttcgtgg tggtgcgctc catcgtgaag
    14401 gcgcacgagg cgcgggtgct caccgggttc gcgggcatgg tgggcgagcg cggcacggtc
    14461 ctcgtcgatt tcgagcggac ggggaaggtg ctggtgcatg gcgagatctg gaacgcggaa
    14521 tcgaccgatc cgctgcggaa aaacgacgcg gtgatcgtcg agcgcgccga tagcatgacc
    14581 ctcgtggtca agaaggtcca ataatatttc aaggaggcgg gaatggacat gctcagtttg
    14641 ccggtaacgt tgatacttct gggggtcatg gccctcgtca gttccatcaa gatcatcaag
    14701 gaatacgagc gcgcggtcgt ctttcggctc gggcggctcc ttcaggcgcc gcgcggcccc
    14761 aaggggccgg ggatcatcat catcattccc ctgatcgaca agtgggtgcg ggtgaacatg
    14821 cgcctcatcg cgatggacgt tccgccgcag gacatcatca ccaaggacaa tgtgtcggtg
    14881 aaggtcaacg cggtggtgta cttccgcgtg gtggagccca accgcgccat caccgaggtg
    14941 gacgactatc tctacgccac ctcgcagctc gcgcagacga cgctgcgcag catcctgggc
    15001 caggcggagc tcgacgatct cttgagcaac cgcgagcgga tcaaccacga gctgcagaat
    15061 gttttggaca gccagaccga tccctggggg atcaaggtga cggccgtcga ggtgaagcac
    15121 gtggacctgc cgcaggagat gcagcgcgcc atggccaagc aggccgaggc cgagcgcgag
    15181 cgccgcgcga aggtgatcca cgccgagggc gaggagcagg cggcggcgaa gatcgtggag
    15241 gcggccaagc agatgcagca gtatccggtg gccatcaccc tgcggtatct ccagaccctc
    15301 aaggaggtcg cggccgagaa gaactcgacc acgctcttcc cgatccccat cgacctcttc
    15361 gaggcgttca tgaagaagct caagtagggg cgacgggatg tcgcgcgcgg ttttcacgag
    15421 ggcgggcggc gcattgctcg cccttttcat tttcgtttct ttcacggcgt gcggccggat
    15481 aacggacgcg gtcggagcgg atgatccgtg cccgggcatc agggcgcggt tcgatgccgt
    15541 tttggagcgg gcgtccggag cgtgcgtaca gtcgaacgaa tgcggttgct acggcgccat
    15601 cacggagaag gcgggttgcg gcggcgtgac cgacaagggc acagccgatc agctcggggc
    15661 catcgcgatg gaattcaatg ccgcgaagtg cgcctactcg cgccggtgcg ccccgtggct
    15721 ctgcgcgccg aagtgcgtga acgggcgctg cgtgcgacgt tgatattgtg cttgcccgaa
    15781 tcggcaccgg cggtgtcacc ttgtccgcat aacacggccg cagggggacc atcatgccgc
    15841 acctgagcag acggatcggc gccttcaccg aatcggtgat ccgacgcatg acccgcgtcg
    15901 cgaacgcctg cggcgccatc aacctctcgc agggctttcc cgatttcgat ccgcccgagg
    15961 agctcgccgc cgccctcgcg aagaccgcgc tcgtcggacc gcatcaatac gccgtcacct
    16021 ggggcgcgcc gaacttccgc gcggcgctgg cccgtaaaca gtcccgcttc atgggcattc
    16081 ccatcgatcc cgacggtaac atcgtggtca cctgcgggag taccgaggcg atgatggcgt
    16141 cgatgctcac cgtgtgcgat cccggcgacc gggtgatcgt cttttcgccc ttttacgaga
    16201 actacggggc cgacgcgata ctctccgggg ccgagccggt cttcgtgccg ctgcgcccgc
    16261 ccgatttcac cttcgatccc gccgatctcc ggcgcgcctt cgagaaacgg ccgaaggcga
    16321 tcatcctgtg caatcccgcg aacccaaccg ggcgcgtctt caccgagggc gagctcatgg
    16381 cgatcgcgcg cctggccgag gagttcgacg ccttcgtcat caccgacgag gtgtacgagc
    16441 atatcgtata cgccccgcac cgccatgtct acttcgcctc gctgccggga atgcgcgcgc
    16501 ggaccatctc ctgcagctcg ctctcgaaga cctattcgat caccggatgg cggctgggct
    16561 atgttatcgc cgatccggcg atcatcgacg gcgtgaaaaa ggtgcacgat ttcctgacgg
    16621 tcggcgcggc cgcaccgctt caggaggcgg ccatcgtggg tctggagatg ccggacggtt
    16681 actacgaccg gctcgtcgag tcatatgcac gcaagcgcga agtcttcctc ggggccctcg
    16741 accgggccgg cctgcgctat acgcggccgc agggcgcgta ttacgtgatg gtcgatgtct
    16801 ccgagttcga tgtcgagagc gatgtcgagt tctgcgagtg gatggcgcgc gaggtgggcg
    16861 tggccgcggt ccccggctcg agctttttcc gcgaggatgt gcgccatctg gtccgctttc
    16921 acttcgccaa gcgcgaggag acgctcgccg cggcggggga acgcctgcgc tcgctcaggg
    16981 aaaaggcgcg ggggcgtcga tgatggcgct cacggcgctt cgcgcgatca tcgcgtcgct
    17041 cattgcgtgt tgcaccctgg gcatgctgtc gtgtgggggc ggggccgatc agctcttcga
    17101 gctccgaccg cggaccgact atcgctggga cgattcgctg gcaggcgccg acgggatccc
    17161 cgcgctggcg cacgatgccg acagcgcggg ctggcgcccg ctcgacggtg acggcgattt
    17221 tcgcataacg ggcgagcgat ggctctggtt ccgggtgaag cttcccgacc ggagttggcg
    17281 cgatccggtc gtcgtcatca gcgagataac gcagtccttc gatgcctatt accggggagc
    17341 acgcatttat cgcttcgggt cacccgacga gctcctgtca cagtacgccg cgcatctcat
    17401 cgacctgccg gacggggccg cgggcgggta tctctacctg cggatgtatt cgcggctctt
    17461 caagatcggc gtgtcggggc cgctcttcga gatcggcgag cgcccggcgg tgctctcgcg
    17521 catcgtgcgt cacgacgcgg acaaactcct gctgggattt ttctttctgg tcctcgggct
    17581 cgcggcgttc ttctactact cgtcgcagat cgactggcag ctgtccatct cgctgggggc
    17641 gctctcgctg ctcgcgggaa tatacacctt ctgtcagacg gacatcaaac aggtccttca
    17701 ttttcagaac catgccttct ggggcgcggt ggaaatattc gcgttctttt tgatctttcc
    17761 ggcgctcaac accttctatc tgcgcgtcaa gcggacgggc gcgctgcgca tggtcgcggt
    17821 ctgttttcgg tggatgtcga tcgccgtgcc ggttggtgcg gccgtcgccg ccgcgttcga
    17881 cgtaaacctg ctggcccgcg cggtccttcc cttcgaggtg ttctgcgtcc tcaactgctg
    17941 cacgctcctg gccctgtcgc tggccgacat caggcgcgcc gaacgcggca cggcgctctt
    18001 cgcgtgcggg atcggcgcct ttctgctcac cgccatcttc gatgtcctgg tggacgccga
    18061 ccttctgccg ggcgtctatt tctacacgat gcactacggg ctcttcgcgt tcgtgatcgt
    18121 gctcaaccga atgctcgcgc gctcggtggg cgagctctat cgcaatctga agagctacgc
    18181 cgacgatctc cggatgaaaa acatcgtcgt ggagaaggcg cacctggaac tctcgtcgct
    18241 ctacaccgag ctcgacaata cgcagcgcga catcgtgtac cggctctgcg acgtgatcga
    18301 gctgcgctcg cgggagaccg ggggccatgt gcgccgggtg gccgagtaca ccgacatcct
    18361 cgcgcgcggc tacgggttgc cggaggagga ccttcgggtg ctgcgcatcg cttcatctat
    18421 gcacgacatc ggcaagctcg gcgtccccga cgcgatcctc gccaagccag gccccctcac
    18481 cgacgaggag tttcgcacga tgcagctgca cacgatcgcg ggctacgagc tgctcaatat
    18541 ctcgcagcgg cccatcttcc actcggccgc gatcgtagcg cgcgagcacc acgagaagtt
    18601 cgatgggacg ggctaccccg accgcatcgc cggcgacacc atccacgtct acggccgtat
    18661 cgtcgcggtc gccgacgttt tcgattcgct ctcggcccgt cgcagctaca aggagccctg
    18721 gcgggacgac gatatcttcg cgttcatgga tacgcaccgg cgggagcatt tcgatccgga
    18781 catggtgtcg atcctgcttt cgaatatcga cgcggtggtt gcggtcaaga agcgttttgg
    18841 cgagggttga tccggcgcgc cgcgctctca ctcgatgagc ttcttgaggc gattgagctc
    18901 gttgagcgcc tcgagcgggg tgatggcgtc ggtgtcgatg ttccggatcg cctggatcac
    18961 gcggtggttt gcggcgttga agatttccag ctgctcggcg gccgcgggtt cggggatctc
    19021 tgttttcttc ttcgattttt tctccagccg ctccaggatg gccgccgcgc gcgcggtgat
    19081 ctccttgggg atgcccgcca gcgacgcgac gtggatgccg tatgatttgt cggccgcgcc
    19141 gggcaccacc ttgtggagga agtcgacccc gtgcagcccc tccttgacga gcaccgtgta
    19201 gttggcgatc ccgtccttgt tccccaattc cgtaagctcg tgatagtgcg tcgcgaagag
    19261 cgtcttcgcc ttgagataac gcagcaggta ctccaccgtc gcccaggcga tcgcgagacc
    19321 gtcgaaggtg ctggtgccgc gcccgatctc gtccatgatg ataaggctgc ggtcggtcgc
    19381 gttgttcagg atgagcgcgg cctccgtcat ctccaccagg aaggtcgatt cgccccgcgc
    19441 gatgttgtcc gaggcgccga tgcgcgtgaa aatgcggtcc gcgatcgaga ggtccgcggc
    19501 gcgcgccggg acgaagcttc cgatctgcgc catgagctgg atgatggcgc tcatgcggat
    19561 gtaggtcgat ttgcccgaca tgttcggccc ggtgatgatg aggatcgtat tgcgctctcc
    19621 gtcgagatgg atgtcgttgg ggatgaaaac ctccttcgtg tagtatcgct ccacgatggg
    19681 gtgccgcccg tcggcgatcg tcgtgcgccc ctcgtcgttg aaggtcgggc ggacgtagcg
    19741 gttctggacc gccgcgctcg ccagcgagca caatacgtcg agctccgcga tcacgcgcgc
    19801 ggcctcctgg agcggtgcgc ggtgtgcgga aacatcgcgc agcagcgtgt cgagcgccgc
    19861 cgtttctatc gccacgatct tttcggaggc cgagaggatg tccgtctcga acttctggag
    19921 ggtctcggtg gtgtagcgct cgccgcccac cagcgtctgt ttgcggaggt aatcgtccgg
    19981 ggctttagcc gtctgcccct tgctgatctc gatgtagtag cccaggatgc ggttgtactt
    20041 cacgcgcagc gtcgggatgc ccaggcgctt tttctcgtcc tcctgatagg ccatgatcca
    20101 gcccttggcg tcggtcttga gcttgtagag gcggtcgagc tcctggtcga acccggggcg
    20161 aatggtgcgc ccgtgctccg gcgagagcgc cggttcgtca tccatggcgg cggagatcct
    20221 gcgcgcgagt tccgcgaggt ctgggatcga ttcgcccagc gccgcgcaca aggcatgggg
    20281 ctgctcgcga aggatatgcg tgatggcgcc cgccgcctcg agcgaacgcg caagcatgag
    20341 gaaatcgcgc gggaaggatc ggcccatcgt gatgcgcgcg atgagccgtt cgaggtcgtg
    20401 cacgcccgcg agcgcgtcgc gcagccgcgc gttcagttcg tacagctcga agaaatgctg
    20461 gacgaggtcc agccgacgct cgatcccgtc gcgcgcgacg aggggttgga ggatcatccg
    20521 ctcgagcgcc cgtcgcccca tgggggtccg ggtgtgattg agcaccgaaa aaagggtccg
    20581 attgcgcgag cggtcgttct ggttgcttgc cagctcgagg ttcgcgatcg tcgcatcgtc
    20641 cagcaccatc gtattcgacg cgacgatgcg cagcggaaac ttcagatggc cgaaggcctt
    20701 gcgctgggtg tccttgaggt actggatgat ggagcccgcc gccagtatct cgatatcgcc
    20761 gcccagcccc agccccttga ggttcgcgat gccgtaggcc tcggcgatga cgcccgcgag
    20821 gtagtcggcg tcgtaatacc agtcgttgat gcgattgacg ggaatgtcct ggctccgcag
    20881 ataatcgccg aagcgcgcgt cgtccggggc ggacccgtcg cgaaacacca cctcgcgcgg
    20941 ggagaagcgg acgatctcgc cgcgaaacag ttcgatggac ctgtcgatcg ccgagaggaa
    21001 gaactcgccg gtggagatgt cgacgaaggc catgcccacc tggtgttcgc cgacgacgat
    21061 cgacgcgaga aaattgctgt cgtcgctttg cagcaggccc gattcgacca gcgttcccgg
    21121 ggtgacgacg cgaaccactt cgcggcgcac cacggtcccc gagctcggca cggcctccat
    21181 ctgctcgcag atggcgaccc ggtagcccgc cttgatgagc cgcgcgagat agctctcggc
    21241 ggcgtgatag gggatcccgc acatggggac gtcgttctgg cgcgcggtga gggcgatatc
    21301 gaggaccgcc gaggccgtgg ccgcatcgtc gaagaacatc tcgtagaaat cgcccatgcg
    21361 gaaaaagagg atgtcgttcg ggtgctgttt cttgatctcg aggtactgct tcatcatggg
    21421 cgtcagcttg tcgctcatgc tcactctccg taaatgtact gccgattaag gttcttcatc
    21481 acccgcacca cggtgccgtt gaagccgtac tcctcggcaa gccggatctt gtcggattgc
    21541 gccgtccgcg tgctcttcgt ccgaccgacg tagatcgtga aaccgctgcg aagcttcacg
    21601 acgcgcaccg gggaaaactc ccggcccacg agcttcttga tcttgagcgc ctcacgcagg
    21661 ttcaggaacg gcccgagcga caccgcgaag gtcgtggcgg cctcgtagtc ctcgtcgtcg
    21721 ttgtcggtca cggacatctc ggggtggatg tcgatggagt cgctccccga gatcgtcttg
    21781 tcgtcgggca ggtagctcac ggtccggggt ttgtatcggg cgagcgcggt gatccgcttg
    21841 cgcgcgtagg cggcctccgg cgagcgcggc cagcggccgg tgacgtcggc gtagcaggaa
    21901 tgggcacggt cgtagtcgcg cttcgattcg tagtatcggc ccaggaggta gatcgccgcg
    21961 ggcaccgcgt ctgcgtcctt gaatccgacg acgatctccc gcagcgacga gaggtacgcg
    22021 cgcgagtagc ccgagcggct tcggtcgata tacgaggcca gcaggagcgt tccggcgagc
    22081 gtctggaaat gatggctggt tttggcgatc tcgccggtcg atcgcgctgc gtcgtcgtat
    22141 cgctccgtgt tgatgtcggc ctgtgccgcg aagaagagga agtcctcgcg caatcggctg
    22201 tcccgaaaca ccttgagggc gaaacgcgcc tcgacgccca gccgcttcca gtcggcggag
    22261 agcgtgagga tctcgcatat gcgatgctgc gcacggtcgc gcttcggaaa gcccgggtat
    22321 ccgtcgacga tcgcgcgcag ttcgcgaagc gcaacgccgg gggccgcctc ggcgagggcg
    22381 gccatcagtc ggacatcggg gacatgcggg cttcgggggt ggtttttcag atacgcgcgc
    22441 gccgcctccc gaagcgcgtc gggcgatgcg gaccgatagg cgctctgaac gcgggcgtag
    22501 tcgtcgcgtt cgtgcgcccc atgcgcgagg tgcggcgata tcaacacgca tcccaggagc
    22561 gccgctcccg caatcgcgta tctcgtcctg gcggcgcgcg tcctcatgtc attcgacccg
    22621 ctcgccgtag agcgtgttcc cgcgcactcc ctcgatgcgc acgcggagca ccgcgccgat
    22681 gtcgcccgcg ccgccgggca gcaccaccgg atgattgagc gcggttttcc ccatcacctc
    22741 gtcgtccgat tttttgctca cgcgctcgac gatgacgacc tccgtggcgc ccgcgcggac
    22801 ggcgagccgt gttcgtccga tcgcgcgctg cagggcgatg agctcgttga gccgcgcgat
    22861 cttctcctcg cgggtgatcg attcggccag ggcatgggcc gccgtaccct cgcgcggcga
    22921 gtaggcgtac atgaacgcgt cgtcgaatcc gatcgattcc accgcgtcga gcgtatcgcg
    22981 aaactcggca tcggtctcgc cggggaagcc cacgatgagg tcggtggaga tggcgcaatc
    23041 gacgagggcc gcgcggatgc ggtccacgag ggagcggtag gcgtctaccg tatagcgccg
    23101 gttcatgagc ccaaggatgc ggtcggagcc gctttgcatc gggagatgga tggcgcgcgt
    23161 gatggccagg tgatcgcgca tggtttcgat gatatcgtcg gtgaagtcct tcgggtgcga
    23221 ggttaaaaag tttactcggc ggataccgtc gatttcggaa atcgaggcca gaaggcgggc
    23281 gaatgaaata tcaccgctgt cctggccgta ctggttgacg ttttgtccca ggagcgtcag
    23341 ttcgatgacg ccgtgatcgg ccaggcgttt cgcgtagtcg atgatctccg ccgacggaaa
    23401 cgagacgagc ggcccgcgga cggccgggac gatgcagtag gcgcagaagt tctcgcagcc
    23461 gtgcgtgatg gtgagccatt tgtgccacgg ggtcccgtcg gcatctccga tgttttcggg
    23521 gttgatgcgc gtcgcgagac gtgcgggctc ctgggaaagg tggaccgcgc ccgcgccggc
    23581 gtcgagatgg tcgcggacga tccggccgat gtccggcgtt tgatatggcc ctacgacaat
    23641 gtgggccgcg ctccccgcga ggcctgcgcc gatgcgctgc gccatgcatc cggccaggac
    23701 gatgatcccg tcacgctcgc gaatcgccgc ctgcgccgcg cgcatgttcg ccagcgcccg
    23761 gtcctccgcg tgcgcccgca ccgagcaggt gttgaagatg cggatgtcgg cggcgtcccc
    23821 gtcggcggga tcgaaaccgg caccggccat cgacaacgcc atgagctccg agtcgcactt
    23881 gttcatctgg cagccgtacg tctcgatgtg gaaggtcctg cgcatgcatc tccttgaaca
    23941 ggcgtcgtgg gctttgtcaa gcggttaacc tcccgggccg gtccgaaata gttcaaggga
    24001 ttggcccggg aatcgtattc ccgggcaggg acgtccgttt cgcccgaata cgtgggtgcc
    24061 ggagaaatcg atggcaatat caacgacgat aggtgtgcat gtagtcacgt gagtgttgca
    24121 tgtgctgttc ggtgcggatg agtacgagct tttacggatg agaggaagta aagtggaaaa
    24181 ttctgcgccc ccccttcggc ttttcttttg cttttgcccg tgaatacttg acaagttgtc
    24241 acctttaagc tgtattcaat tcatgaaaac gctaccggtg ggcgaattca aatcgaaatt
    24301 ctctgaagtt cttgaaaatg tgaagaaaag agatgaaatt gctatctcct tcgggaaaaa
    24361 gaaagagaaa atcgcaatgt cctcctgtgg acactcttcg agtgcagcaa tacatggact
    24421 tcggactaaa agtaacatgg tgacttttca actgcgcccc ccatcccccg cccgcaataa
    24481 tggttgaccc cccacgccag gcgtcgtatc cttcctgaaa catgggaggc gcgccatgag
    24541 ctgttatttc cgacatcttg ccgatatcct tgccgaatcc ggcatcgtcg tcgacaagga
    24601 aaacaagaag gacgtggacc gcatgatcca cgaatatctc gacgtggaat acaagaactg
    24661 ctccgatgcg tggagggcct tcaaggagtc cgtgcgagat gatgatgcgg cgcgcaagaa
    24721 gtttatcgat cacttgaaaa aataccgtcc gcgagattga gtatttctgt tgcataataa
    24781 tcgcacatgg taacgatgcc gcttcatgaa aactaagccg atagttatcg tgaccgcggg
    24841 cgacgggccg ttcgcaaacg cgaaacgggc ccttgcgcag cttgatctcg caggcgttcg
    24901 cggaaagcgc gtgctcgtga agccgaacgc gggccggctg gtcgggcccg cgcgcggcat
    24961 caacacgcat ccggaggcgc tcgctggcgt catcgagtcg ctccgcgagg ccggggccgg
    25021 cgatatcgcg atcggcgaga gccccatcct cggggtgaaa acgctggagg cgttcgaaaa
    25081 atcggggatg gcggaggtcg cgcgtcggta tggatgcgcg ctcatagatt tcgacgcggt
    25141 cccgcccgtc gagaaggcca tcccggacgc ccgtgtcgtc acatcgacca aggtgtgcgc
    25201 gcccatctac gaatacgacg tcctgctctc gctgccggtc gcgaagtgcc acatgcacac
    25261 cggcgtgacg ctggggatta aaaacatgaa gggctgcctc taccggcgcg agaaggtgcg
    25321 ctatcaccaa ctcgagtacc cgccaggggc ggcgtggggc gaacggacgc tcgacagcgc
    25381 catctccgat cttgcgatga tcctcctccc ggatattacg gtcatcgacg gatcggtcgg
    25441 catggagggg ttgggcccat cgggcggcga gcggatcgag tcggactttg cggtggcgtc
    25501 gttccatcca ttgggggccg atgtcgtcgg ttgcgcgatg atggggctcg acgccgccga
    25561 ggtcgcgcac ttgcgactcg tcgcggagcg tcgcggctgg cccatcgaca ccggggccta
    25621 tgatgtgcgc ccggccgatt ttcgcgcgca tcatgccgat tatcgcaaac cccccacgga
    25681 catatcggtc gcctatcccg acgtggtcct ctacgacgtg gagtcctgca gcgcctgcct
    25741 ctccacggtg atgctgtttc tcaagcgttt caaggacgat atgagccagt acctggtgga
    25801 cggcaagctg cacatcgcga tcggcaaggg cgtgcgcgag ctcgatctgc ccgaggggac
    25861 catctgcgtg gggaactgcg cgaagcggtc ggggacgtgc tttttcgttc cggggtgtcc
    25921 gcccgtgccc acgcgcatct acgaggccat caccggcgag gagcccgaca agaacgagcc
    25981 cgatgtttca tagcacgaac aggagggagc aatgcgaacg aatagaatcg ccgtcagcgc
    26041 ccatgtcgta aacagtatgc ttcttgtcgc catattcgca ttttccgttt gcgtattcga
    26101 tcaattgccc aatcgcgttc ccgcgcatta taatttcgcc ggagtgccag atcggtggac
    26161 cgacacctca atgatgggct ggctctcggt cccgctcctg ttcgccggca tcacggcgct
    26221 cttctacggc attgcgctca cgctgccctg gttccggaag cgcccccagt ggatcaacat
    26281 gcccgaggag agcaagaagc gttttctggc gctcgacgag gaggcgcggg gcgcggcgct
    26341 tcgcggcatg gcggcctatc tctactggtt tccggtttcg tcgagcctca tgttcctgtg
    26401 gatgcgctac gaggagtatc gcttcatcgt tctcagggag ccgtccttca atggggcgtg
    26461 ggccatcggc atcattatcg cggggcttgc gatcacgacg atcgcgatgc tgcgcgggat
    26521 caagcgcctg gtgtaggcgc ggggtctatc gcgccgcaat cgcggagttg cgcgatggcg
    26581 tcatcgtcgt accccagagc gcggaggatc tccaccgtgt ggcgtccggg cgccgttccg
    26641 gcccatccgg cgcagttgtc gcccgacgca aagcggatgg ggttcccgat ctgcgtgagg
    26701 cccgagcccg cagcggtgcg cacggtcacc agcatcccgc gctcgctcac gggcggccgc
    26761 gaaaccgctt cggccaaagt gagcaccggc tccacacagg catcgacctt cgaaaacgcc
    26821 tccatccatt cgtcaagcgg cttggatgca attcttaatg ccacggcctt ctttacgtcg
    26881 tgtatgtttg
//
LOCUS       DAORHF010000025        26525 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1132_length_26525_cov_6.355610, whole genome shotgun sequence.
ACCESSION   DAORHF010000025 DAORHF010000000
VERSION     DAORHF010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26525
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1132_length_26525_cov_6.355610"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            475..1545
                     /gene="prfA"
                     /locus_tag="PKJ16_03840"
     CDS             475..1545
                     /gene="prfA"
                     /locus_tag="PKJ16_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HNV46149.1"
                     /translation="MENKLEEIARRCAAIEQELSRPEVIGDQNKFRELTRQHSQLAPV
                     VGAFADYRKMKQELADSQELLKSEKEREMREMLAEETAALEGKIAAAEEALMVMLLPK
                     DPNSGKNILMEIRAGTGGEEAALFAADLFRMYTRYNDRMGFKMEIMELSPTGIGGYKE
                     IIFSVAGSEAFDNLKFESGVHRVQRIPTTESGGRIHTSAVTVAVMPEAEESDIAIKPD
                     EIRIDVYRASGHGGQSVNTTDSAVRITHIPTGMIVTCQDEKSQLKNKTKALRVLRARL
                     FELKERERQAEESKLRKSQVGSGDRSERIRTYNFPQSRVTDHRVGLTLHSLATVLDGE
                     LDEIIEALKKNEVDERLKASAE"
     gene            1674..2876
                     /locus_tag="PKJ16_03845"
     CDS             1674..2876
                     /locus_tag="PKJ16_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46150.1"
                     /translation="MHITTSLLLAAQLVLGMGSEVRSLAEFAPASEKDFGIVMYLKGA
                     PIMGFVEKTKLIDALVDRDIKKKKMSPEQAAMMRMQSAIQLQLITGIAQKITEGIMFL
                     YADPADIAAKAAGKEKLLVLANLALAKEPAREHPMLVGVHNLKEGEIDTATFGSPVTR
                     FGTVVAGSSRADQLTAFINNKKQSSRVGKGAIAALDGAIGSNAVYLSMNNLDAFEGQA
                     DLHKLAATDEKQNATLVNGLNKHAGGKKMTTADIDKMIADMKTFEFMTDFKSDTVLMT
                     AVMAGKTPAHGALLKKVSDDDEIWRESYRANGLEDMVDRIKHESRLEERAPGYRLTVA
                     IMMANDDFKELILRSAERVTEKAKAKPGGEGAAIQAPASVAKPKTAKPDTKKKKKVPK
                     KKRKIKNL"
     gene            2897..4456
                     /locus_tag="PKJ16_03850"
     CDS             2897..4456
                     /locus_tag="PKJ16_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46151.1"
                     /translation="MRIAGFALAIFLISSAARAHGPGVGTPSCKPSCSNCWGALKGCL
                     TYPFSSSSSGPSRVKLMVGGIEATFRNHTLYYFTLPDGTNGPLQRGHLANQHDLSCGD
                     RRIRFMPGAVSFHRNGSVARGCLASRTDFPLRDSAITLEPQCVSLYASGRLELFSLTR
                     EAELPFRGGRTRFEPGPMALHESGSVREGTIATDTEIVAAGRTFTVGKIHHAIFHEDG
                     MIERIYVKPQYRDRRIVARYGGRDLPFHILWLHPSGRLRKGFLAENAEFTVGPNRLLA
                     SAITPNYFENREILSLDSTPSVSFFDDERIESVHIAGEPVITAHGTRMRVKDGIEFFR
                     NGSVRSTTLAERVSLPLGGGRRASFAWNRVVFYPNGAVREGHVDSTSAVVINGRSVTF
                     TEKSSARTLFHPDGTVHSTDTFNKKYRSWGFTYPAIYADFSKGIVEAEGNFHHTGEPI
                     SLTEFGRDKTETPIVITLPRSLRCYYWIRYVHPGGKRLADAIQVSEDTVIDINGRKVP
                     ATAFTWVKLKP"
     gene            complement(4487..6172)
                     /locus_tag="PKJ16_03855"
     CDS             complement(4487..6172)
                     /locus_tag="PKJ16_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46152.1"
                     /translation="MKKFPVSIDIKGKTFIDEWDRLELSPDGSSVLMVAMLQKERKDG
                     FDYSRYVLVNDRLYGPYAHARGAFFGAHRFFVSYERGKSMYKRCVQNKCTVYPAFVCV
                     SLIPPIGKLLEYDFVKRIADPPEQRGFLVDGGNIASSNPWFSQSGFVANNDGSLYAFK
                     YGEGYKRSIYFVRVNDTAYGPFNRASKPALSRNGATYAFSYSDEKRRSVRVNGTVHGP
                     FEGHVPDEVSAPVVSADGAHVGFAHGAGANNPYRFFVRVDANTYGPFSKVGEPGSHRK
                     GPWLRFNDAGTVFAFLYRMSREDGSEMGGVRTADRDYGPYKHAVNLRVSPAGDTVAFR
                     YAMRGDRDTERVMVNGASYGPFQPHAFGYFPHANAPVVSKNGKQFLLSFRENDRDIVQ
                     MGARRLGSFYRLTSVPVLSADGARVAFAYKDEFHFVHADGRRYGPYLEAGDIVFGPRG
                     AHLAFTYTAKSGQTYLRVNETEYGPFDYREQHVDRRSLLTVGFAPDDGRYCIRAVRFD
                     PDPARPGMRLRNYYLHTKEAGYGPYQYAAYRFDERGRLIIAYLKDGFVHIEEI"
     gene            complement(6392..7918)
                     /locus_tag="PKJ16_03860"
     CDS             complement(6392..7918)
                     /locus_tag="PKJ16_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46153.1"
                     /translation="MPKGPSFCLYVLLWPFTALADAISKSYREHTTVRMTVGGCEAVF
                     RTEGLVAWNTGDGTGLRTGNLAREAMLNVGGKRLRFSPGWTTFHESGRVKTGNLADGG
                     ETRTALGPVSVKAGPTAFYENGTLRECVLQRDTAFTVRERRVLFEPTEKDGLGFHDPE
                     SSGCASDKSPGYCFYGTIEFYESGVVRAGYPAAGQTLPVDGGELRFPRGAAASFREDG
                     RLSGVYLKSGVTIQAPCAGRLVSFMRYVVFHDNGRMKSGVAAAPSTLRFGKEVVAVAT
                     GDIVRLHENGALKSAGSGSVRDYVVRGQVLRLGSVSFHDDGSVHAGELGGYHEVVVGI
                     SPARRLPIGITGNIEFYRSGSVKRAAIRERSTLDINGRRETIDAPAEAVFFESGEVMS
                     ISVEGIRGTRTFHLGGKPVGDIPNGTLVMFRDYRNGIIEGMSRGNCHSILGLRGDRAG
                     KRVTIPREIPDFWVRYADADRRRIDGLLLDRDAVILVGAEKRACRAFTWLPLDAELLR
                     "
     gene            7977..8741
                     /locus_tag="PKJ16_03865"
     CDS             7977..8741
                     /locus_tag="PKJ16_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46154.1"
                     /translation="MTIVRHVFFEDGPIAHDYHHAGGIASTNPHGPFTLHATQAMAHD
                     PERAPAERRTRPRSLARNVNRPGEHATRIIHKKVIDRTAAAPIHEGPRDKENPGHIGL
                     SARTTPYYGFRSINMKRLMGIIVVLFFIASLPSCSVYNKLRGYVGIGDLKRVANLISM
                     DFGKGPDGPFQVSTIASVDPKLVVKNSTDRTITVKAQGPTAKTFVINSGQQSEGTVKS
                     GMYHFVASAPGVSSAEGDVELKGFKQYKWEFIIRKQ"
     gene            complement(8995..10083)
                     /locus_tag="PKJ16_03870"
     CDS             complement(8995..10083)
                     /locus_tag="PKJ16_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782456.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HNV46155.1"
                     /translation="MTRSYDSLILHVMSGTGNSLRAARWLADLFGPGGATIVRIAKSY
                     DAPPAPDRNSMTGFVFPTHGFTAPWAVIRHAMRIPRGTGSDAFVVATRAGTWLGFLLP
                     GLSASAMFVTAFLLMLKGYRIKGLRGLDMPSNWTALHWGMSPKNAQRIIDRARIDIER
                     LAALIFSGKTHFVSINNFVELVGGIALANISAGYLLIGRFALAKLFYANWKCTSCGLC
                     ARECPFGAIRMMGTDPKLPYWTFACESCMHCMGYCPEKAVEASHPLAIAMFYVGTFSL
                     AAALGAWIIRLQPGLSGIGAGPGGAFVRSAVNYAYVLVSFFLVYVLFYYLVRIPLVNR
                     LFTYLTLTHYYRRYREPGIGGKDFTEGG"
     gene            complement(10144..10752)
                     /locus_tag="PKJ16_03875"
     CDS             complement(10144..10752)
                     /locus_tag="PKJ16_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV46156.1"
                     /translation="MKLRALILCGIVLLATYQSPAFAAVISGSDVNIRAKPDAASAVV
                     GKLQSGDRVTVLEKTGSMGFAGMYLGRWCRIKSGSREGYVFSSFVREDGDADEQFHVF
                     FQAFAAANEKGDLNYIRSRTVFPLTVVSCFEGNCTDSAKTAATLELNDLYMKRPFMYK
                     QEFTYEKGSVHLFYGYEATRFNLYFRLQKGKWVLYAVRTSSC"
     gene            complement(10927..12513)
                     /locus_tag="PKJ16_03880"
     CDS             complement(10927..12513)
                     /locus_tag="PKJ16_03880"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV46157.1"
                     /translation="MSYEKLSVALMLALALVSCRNAENRYYTWVDKAALREAPSLDAP
                     VVAWLDQGEDVEFTGERSKHTTNVELRHQRFDRHWITVKTASGHTGWMHEATLKPYGE
                     LLKRVANERDWNPEYESILLPLIKGNAREFAKAAASMRVPGAPEPGMPAKNFPRLMHQ
                     YAGYTDNPAIREMLRSKVRHYYQPARKYFQSLPAVPPGHAAIASGSVIAALTDGDYFL
                     PVFSPNGRRLACARKAIRRGVTEYSKNKNDPHDFVTGELILLDTSTWQPTLCLQADAC
                     AKYRVYAAPIIGIEWRSNDRLTVTLHDGDVDNIELTVDALSCRILKHEVDGGGERGYE
                     RSRRELEARLAPYQPADAPLAFESRNRALVTGAFIVQDFTDNGSRSVLRMLDFSKKAV
                     RVIRPVPDFHDGALNGAARMGDDFILALNEAWFWNERFYVLRYREGKTPSVLLALDVN
                     EQYPYLRETGMIRGRALLRISGSPAHAGYYEYAAGRLLRATDFAVIDSIVVSSDGALA
                     AFCYREKPGACDRRIAVKRL"
     gene            complement(12696..13913)
                     /locus_tag="PKJ16_03885"
     CDS             complement(12696..13913)
                     /locus_tag="PKJ16_03885"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV46158.1"
                     /translation="MRALKTEGYEVPTPIQERAIPYILQGSDILGCAQTGTGKTAAFA
                     LPILQNLWHANSAGARPRTLRTLVLTPTRELCIQIAESFAAYGRHTGLRHAAIYGGVS
                     QKPQTDALRAGVDILIATPGRLLDLMSQRLVSLQAVTSFVLDEADRMLDMGFINDVRK
                     VIAKMPRERQTLFFSATMPAEIRKLADDILTEPVPIAVTPVSSAVDTVEQRIYHVAKA
                     DKKALLHHVLKDTSIDAALVFTRTKHGADKVARDLEKAGIRAEAIHGNKSQGARQRAL
                     GNFKARKTRVLVATDIASRGIDVEGLSHVINFELPNEPETYVHRIGRTGRAGLSGIAL
                     SFCDVEERPYLTDITKLLARPIAAVEEHPYRLEVGAHVPVPAAKAVKRPGASGSRPPQ
                     RSNNANRGRRRYR"
     gene            complement(14017..14304)
                     /locus_tag="PKJ16_03890"
     CDS             complement(14017..14304)
                     /locus_tag="PKJ16_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46159.1"
                     /translation="MLRQNIITDKRKSKTDHEFLTTKQEFAQIVRNKTLSSGKQHGHV
                     KEELLRGIEVVESFIAAGLVRDRKGRLFRYEPEDARDAVASGRYEWFTWNC"
     gene            complement(14639..15505)
                     /locus_tag="PKJ16_03895"
     CDS             complement(14639..15505)
                     /locus_tag="PKJ16_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46160.1"
                     /translation="MMKRKNGPGRCLGDADIARFVDKTLPADRRRAAIEHMADCAQCR
                     SAAIEVGRLAALPGHRVPPDLEQRAFAAARASRRIIPLRFALPLAAAALVIMASVVAI
                     NIINDTPQEGIDLASSAPAVAIDAGNAPKTARVASRRGAAAPSAAPDMPRVAAKDPRR
                     STPHGDGTSMLRAGWAYFQLSSTQAGDTKLIETLSRDLAAAKLRRDEGVDLVITQQYP
                     EFLEKLARLPREEQKEFIDGYVLSMLFYLDEKGGLKRECMAKSRDLLADASGDTVRDV
                     MLYSPECALPER"
     gene            complement(15505..16188)
                     /locus_tag="PKJ16_03900"
     CDS             complement(15505..16188)
                     /locus_tag="PKJ16_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV46161.1"
                     /translation="MKAGAGDTSGFDDPRGEARALRALDDDALMEHLFREYREFIYAI
                     ARAKIWTARLPREDADDCFADIIVRVCDNGCRKLRQFRGGSSFRTYLATIVRNLATDY
                     IRRERRTRDRLREFDEFIVPEAVDEALGSGGQSRSPEAQVLESQRAEAMRDALDALRG
                     ALARLDAEDRLIVALRMEYGMSYREIDEHLGIDNARYRLTKIHQELRLSFDAGTRMRF
                     EELLREEGL"
     gene            complement(16429..17970)
                     /locus_tag="PKJ16_03905"
     CDS             complement(16429..17970)
                     /locus_tag="PKJ16_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46162.1"
                     /translation="MRTQCRIAVMLCLLLCWPRESSARDFPVHIKSDPQKTHLVISPD
                     GSRAALVHLASAGYVNTGYAVQLNGDYRDIDAEWAGSPVFSATGIMAYDRSDKRGKSA
                     VIVDGRGNARRIVGASGIRINRDGSNYLYKKPMPGSDRSFLCDTDRCRGPFDEVTWYG
                     FNPEGSLFGVVAREIAAPQRERAPILRNNPRAVEKPDPRNRVIINGRSFGPYEIVFDK
                     PVFSDRGGRYAFSYLEGSAYYCVVDGMTLGPFDTQRDAVFSPDGSRYYLVSRDNRNQE
                     LFIGDRRIAGPFRSIDRLTLSDTGGSFAFIGRRAGPIDTCYNYGCAPVVLLGRHADHC
                     CSFKWLNVMVSTGPGDVAGRYSAFVQFNDRRLSGFDSASGLAINRAGNSIAFVLERSD
                     KKFVHFNNREYGPYDFVLGPHLDDSGKHLLFYFQVGSLSRGLRYVLRHDDREYEIRDR
                     PIRIGFTADGTRSCCVFEAKKRVYLMVDGISRGPYDAADFVIRENRIFTAWVAKGRLF
                     LEEAR"
     gene            complement(18012..19346)
                     /locus_tag="PKJ16_03910"
     CDS             complement(18012..19346)
                     /locus_tag="PKJ16_03910"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV46163.1"
                     /translation="MNTIKRLITYLVGGKDEFVLEHQILNAMLLLATICAPIITIGTL
                     LLDFGLAHRLIVVFIGACGIGIYYLSRFRRIRLIKTIILLLFGLLSVIWFTGYGSRGG
                     DPLFFLFCAIFSLILLKGAVRYLFFFMTPVVVAGLTLCEHLNPGSVKSYASEADRVAD
                     YLMNYVTWIVVVGFIFKFVVDSYFEEREKLEVRNFIMNRELDMAREVQRKLIPVGPPW
                     ERIAFRYMPMMKVGGDYFDFIRFREADRIGIFISDVCGHGVPSALIASMVKSVVLKTG
                     DERTDPAQFLFGLNKTLFGNTGAMFVTALYGIFDRRDNTFVYSSAGHVSPFHVHDARA
                     DSLSTESIGVPVGIVSNDEIRAKYNPYVNETVRCEPGDKIVLCTDGLLEVLGPQSGEE
                     FFGARALAEVMAKHAALPADAFAGAIVDEVTRFHGGDEFDDDLCLVCIEAGG"
     gene            complement(19418..20128)
                     /locus_tag="PKJ16_03915"
     CDS             complement(19418..20128)
                     /locus_tag="PKJ16_03915"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HNV46164.1"
                     /translation="MANIITITSRLIPPAVALACAIAAPGMLGQGTPLGAAPSFPSKP
                     AYEKEDRALNEAFRAIMRRLDAAMKRELRENSREWIRHRDYLCGYQAAMRLGKDDARD
                     DEYYQCLRDLTRDRTAYIRAAFGKTSAPSGVDGAYGDGFGGVMTLRTHTDGSVHFTIE
                     VVRGPTFHLGSISGDIAVRGGTAEFREKEPCAGDGCCRIEFAFGKHWIDVREHACAYH
                     HGARVFFDGRYRRVGKTP"
     gene            20299..21147
                     /gene="prmC"
                     /locus_tag="PKJ16_03920"
     CDS             20299..21147
                     /gene="prmC"
                     /locus_tag="PKJ16_03920"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HNV46165.1"
                     /translation="MNITDLLRQASQRLQEAGVESPRLDAEVLLAHAMGIDRVRIITG
                     GDREVPDNERRSFDSMIDRRARREPAAYIMGAKEFYSLEFEVNERVLIPRPETELLVD
                     LALYHAPRGGRVIDVGTGSGAIAVAVVRNRPDIAMTATDLSGDALALAHRNADALLGT
                     HAIRFLQGDLFAPVAGERFDLIVSNPPYVDPALKGALQPDLAFEPELALYAGESGTAV
                     VARIIRDARDHLADGGMALMEISETMKDFVEREASACGFAVSVLSDYAGLPRVASLTT
                     RGSAHG"
     gene            complement(21170..21409)
                     /locus_tag="PKJ16_03925"
     CDS             complement(21170..21409)
                     /locus_tag="PKJ16_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46166.1"
                     /translation="MEVRDMNGNPGMWEELSWEDLNDTEKELWSALGWSEELWDGNTA
                     PESTDKEWDDLSDRERNAAMGLGFTEEIWNNFEDE"
     gene            21605..22042
                     /locus_tag="PKJ16_03930"
     CDS             21605..22042
                     /locus_tag="PKJ16_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV46167.1"
                     /translation="MDDRLLFLMSRAHHAIKNHVAGEFAREGIGITPAQMGILFSLRM
                     SDGLPMSELGRIIRIDNAAVTRHVDSLERAGCVRREAAPGDRRKSIIRITAKGRDEAV
                     RCARTARRINAMLKEGFTAEEIATFTRVLNGVIEKCSPANGGV"
     gene            22042..22506
                     /locus_tag="PKJ16_03935"
     CDS             22042..22506
                     /locus_tag="PKJ16_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HNV46168.1"
                     /translation="MSQVLRIYNALAGKPFGKWLFSRILCFKAPYFRTIKPRFAALRP
                     GYGEIRMRNRRAVQNHLKSVHAIAMCNMCELVGGTTLEVSIPAHLRWIPRGMTVQYRT
                     IARSDLTGTCAIPDHGAITPGTLPVSVSITDTRGTEVFSAVIDMHISERKRT"
     gene            22643..22867
                     /locus_tag="PKJ16_03940"
     CDS             22643..22867
                     /locus_tag="PKJ16_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV46169.1"
                     /translation="MKITTKGQVTIPQHIREKHGLMPHTEIDIIEEGAKIVIVRSKKS
                     RHSDQFSRLRGTATVRMSTDEILRLTRGQE"
     gene            22864..23268
                     /locus_tag="PKJ16_03945"
     CDS             22864..23268
                     /locus_tag="PKJ16_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010480903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV46170.1"
                     /translation="MKGLLVDSSVVLDIITDDPTWADWSQQMLARHVTSHRLYINPII
                     YTEVSIGFERIEEVEFVISNSGLKILQIPREALFLAGKAFLRYKKHKGSKSSTLPDFF
                     IGAHAAVSQLAVLTRDVTRIRTYFPTVRIIAP"
     gene            complement(23282..26416)
                     /locus_tag="PKJ16_03950"
     CDS             complement(23282..26416)
                     /locus_tag="PKJ16_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV46171.1"
                     /translation="MKIQTPRLLVVALLFFALTGCSAQKTATPRASDDLRSDQKPASA
                     MEKKETMRAAEKEAGMDLDKAMADEAPRGRYKAMAKKDAGGRKGSDVKTWKRSEIAPN
                     TSRLMIGDKEELRLVAMQAKVRIVGFRARVLIDCYFHNEHARQFEGTFKLRLPDDASP
                     YFFAFGETAYAAEDIPKGQMFYSLPDGEKMGMEPEQIMQDRQAQWKQPKEARMVPKEK
                     AAFAYQETVRRSVDPALMEWSGAGIFSARVFPLAPKTVHRVVIGYDVNLLPAGDDLEY
                     RFDIPDVPESMVDVSVRDLPNVTAEVDLKTSPYKAGDATVYRFTNRKKETVTVRLKNP
                     GSVLLHGADGKVGVHFATMFRPPLPKKSDTTAAQKAVFLVDTSLSSNPDQFNVWRKLV
                     EAVLANNRASIPEFAVLFFNIEQRWWKRAFVKNTPDATAELLSAMNALALEGATDIKS
                     ALAEAVSPAWLRRDDGAPWTLFLLSDGAITWGDGNAAALSRVLALKGRGRLFAYRTGL
                     AGSDMGLLGQIARETGGAVFSVVGENEIAGASTAHRMSSWRLVGMTLEGGTDLLLAGR
                     PVSVYPGQALLLAGRAAKEIPANARVVLSLRDAAGKRLQVAAPIRHAVRTELAARVYG
                     QVAVGQLEEFADAVEKYSRAYATHYRITGNTCSLLMLESEAEYRRFNIKPDEDALVVA
                     KNPAAPVIARVMQRIGDSLSNPKKGFLAWLAKMEKMPGVKFSVPTALSDFLKEMPDSS
                     FAITPKPLACSVFRWEQLPLAVRGKIRNKDIEYDAFTAEANRRKAKHGAADALKAVSS
                     LVEMSPGDGVLMRDVGFTAMQWGLGDHAYHLFRRVAEARPFEPHTYHALALLLSEMGR
                     TDLAIAYYEVGLAGQWDSRFGDFRKILMLDYLNLLKKIRKGTARTIDREFAMARLKTL
                     TKEIGISRADLMVTIMWNTDRTDVDLHVYEPSGEECYYSHKNTKIGGELTYDVTQGYG
                     PEMYLLKKSVPGNFTVKVKYFASDSNRKSARTKVYATVYRYWGTKRETIEKKTVTLEY
                     GKDMHDIATVKMAQ"
BASE COUNT         5212 a         8574 c         7929 g         4810 t
ORIGIN      
        1 cagatttttt ggggttgtag aacgtagatg cacatatact atacggacat atagcttata
       61 ttggaatttt tttcttgagc cgttacaata ccggggttcc aacgtccagg acttcatgct
      121 gacgacatgc acggaggcgc tcatgacctt cgcattagaa acatactgtt gcgcattgat
      181 aggcacctat cccgacggcg ctcgacgtgg gcggtacgtt ctgaacgaaa tgcccgaaag
      241 ccgcgacgcc acggatggca aggagcggcg agcggggaga aggggcgtcc gagaggaagc
      301 ggccgcgtga gtcttttccc atcgcagcca tttttccttg acgaaccgct cccgcatccg
      361 atcaatgcca gtggttcaat ccggcgacgc gtgttaccgg gaaccgccca tatgcgcgcc
      421 gcgtgacgat tcgatcacgg cacccacgca cctcgaggac tatagaacgc gaccatggaa
      481 aacaaactcg aagaaatcgc ccggcgctgc gccgccatcg aacaggagct gagccgtccc
      541 gaggtgatcg gcgaccagaa caagttccgc gagctcacgc ggcagcactc gcagcttgcg
      601 ccggtggtgg gcgcgttcgc cgactaccgg aaaatgaagc aggagctcgc cgattcgcag
      661 gagcttctga agtccgagaa ggaacgggag atgcgcgaga tgctcgccga ggagacggcc
      721 gcgctggagg ggaagatcgc cgcggccgag gaggcgctca tggtgatgct cctcccgaag
      781 gacccgaact cggggaagaa catcctcatg gagatccgcg ccgggaccgg cggcgaggag
      841 gcggcgctct tcgcggccga cctcttccgc atgtacacgc gctacaatga ccgcatgggc
      901 ttcaagatgg agatcatgga gctcagcccc accgggatcg gcggctacaa ggagatcatc
      961 ttctccgttg ccgggagcga ggccttcgac aatctcaagt tcgaatcggg cgtgcaccgc
     1021 gtccagcgaa tccccaccac ggagtccggc gggcgcatcc acacctcggc cgtcaccgtc
     1081 gcggtgatgc cggaggccga ggagagcgac atcgccatca agcccgacga gatccgcatc
     1141 gacgtgtacc gcgcctccgg ccacggcggc cagtcggtga acaccaccga ctcggccgtc
     1201 cgcatcaccc acatcccgac gggcatgatc gtgacctgcc aggacgagaa atcgcaactc
     1261 aagaacaaga ccaaggcgct gcgggtgctg cgcgcgcgcc tcttcgaact caaggaacgc
     1321 gagcgacagg cggaggagtc gaagctgcgc aagtcgcagg tgggctccgg cgaccggagc
     1381 gagcgcatcc gcacctacaa cttcccgcaa tcgcgcgtga ccgaccaccg cgtggggctc
     1441 acgctgcact cgctcgcgac cgtcctggac ggcgagctcg acgagatcat cgaggcgctc
     1501 aagaaaaatg aggttgacga acggctcaag gcgagcgcag aataacctga aacaggcgcc
     1561 ggattgcttc gtcgccatcg ctccgcgcaa tgaaaaaaaa cctgttgtgc gacaaaccga
     1621 atatatactc accgatttca attacgccgc atcacgaatc cgagaggtac cacatgcaca
     1681 tcacaaccag cctactgctc gccgcccagc tcgtcctggg aatgggaagc gaggtccgct
     1741 cgcttgcgga gttcgcgccc gcatccgaga aggacttcgg cattgtcatg tacctgaagg
     1801 gcgcgcccat catgggattc gtcgagaaga cgaagctcat cgacgcgctc gtcgaccgcg
     1861 acattaagaa gaaaaagatg tcgcccgagc aggcggcgat gatgcgcatg cagagcgcga
     1921 tccagctcca gctgatcacg ggcatcgcgc aaaagatcac cgagggaatc atgttcctgt
     1981 acgccgatcc ggccgatatc gccgcgaagg cggccggcaa ggagaagctc ctggtgctgg
     2041 cgaacctcgc gctcgcgaag gagccggcgc gcgagcaccc gatgctcgtc ggggtgcaca
     2101 atctcaagga gggcgagatc gacacggcga ccttcggttc gcccgtcacg cgcttcggca
     2161 ccgttgtggc cggcagctcg cgcgcggatc agctaacggc gttcatcaac aacaaaaagc
     2221 aatcctcgcg cgtcgggaaa ggcgccatcg cggcgctgga cggcgccatc ggctcgaacg
     2281 ccgtgtatct gtccatgaac aacctggacg ccttcgaggg gcaggcggac ctgcacaagc
     2341 tcgcggcgac cgatgaaaag cagaacgcga cgctcgtgaa cggcctcaac aagcacgcgg
     2401 gcggaaaaaa aatgaccacg gccgacatcg acaagatgat cgcggacatg aaaacatttg
     2461 agttcatgac ggacttcaaa agcgacaccg tcctcatgac cgcggtgatg gccggcaaga
     2521 cgcccgctca tggcgcgctc ctcaagaagg tttccgacga cgatgagata tggcgcgaat
     2581 cataccgggc gaacggcctc gaggacatgg tcgaccgcat caaacacgag tcgcgcctcg
     2641 aggaaagggc ccccggctac cgactcaccg ttgcaataat gatggcgaac gacgatttca
     2701 aggagctcat cctccgctcg gcggaacggg taacggaaaa ggcgaaggcg aaacccggag
     2761 gcgagggcgc cgcgatacag gccccggcga gcgtcgcgaa accgaaaacg gccaaacccg
     2821 acacgaaaaa gaagaaaaag gttccgaaga aaaaaaggaa aattaaaaac ctgtaagcgg
     2881 gagacaggga tcggccgtgc gtatcgctgg tttcgcgctc gccatttttc tcatctcctc
     2941 cgcggcacgc gcgcacggtc ccggcgttgg caccccgtca tgtaagccga gctgctcgaa
     3001 ctgctggggg gcgctcaagg gctgcctgac ctatcccttc tcctcgtcct cttccggccc
     3061 atcccgagtg aagctgatgg tgggtgggat cgaggccacc ttccgcaacc acaccctcta
     3121 ctactttacg ctccctgacg gcacgaacgg cccgctgcag cgaggccatc tggcgaacca
     3181 gcatgatctc tcctgcggag accgccggat ccgcttcatg ccgggcgcgg tcagcttcca
     3241 tcgaaatggt tccgtggcgc gcggctgcct cgcatcgagg accgatttcc cgttgcgcga
     3301 cagcgccatc accctcgaac cgcagtgcgt aagcctgtac gcaagcggac ggctcgaact
     3361 gtttagcctc acacgcgagg ccgagcttcc cttccgcggc ggccggacgc gtttcgagcc
     3421 gggccccatg gccctccatg agagcggttc cgtgcgcgag gggacaatcg ccaccgacac
     3481 ggagatcgtc gccgccgggc gcacgtttac cgtggggaag atccatcacg cgatcttcca
     3541 tgaggacggg atgatcgagc ggatctacgt gaagccgcaa taccgtgatc gacgcatcgt
     3601 cgctcggtac ggcggacgcg acctcccgtt tcacatccta tggctccatc ccagcggccg
     3661 tttgcgcaag ggcttcctgg ccgagaacgc cgagtttacg gtcggcccca accgtttact
     3721 cgcgagcgcg ataacgccca attatttcga gaatcgcgag atcctctctc tcgactcgac
     3781 gcccagcgta tcattcttcg acgacgagcg catcgagagc gtgcacatcg cgggcgagcc
     3841 ggtcatcacc gcgcacggga cccgaatgcg cgtgaaggac gggatcgagt tttttcgcaa
     3901 cggttcggtg cgctcgacga ccctcgcgga gcgggtgtcg ctgccgcttg gcggcggccg
     3961 gcgcgcgtcg tttgcgtgga acagggtcgt gttctacccg aacggcgccg tgcgcgaggg
     4021 gcatgtggac agcacgagcg cggtcgtcat caatgggcgt tcggtgacat tcacggagaa
     4081 aagcagtgcg cgcaccctat tccatcccga cggcacggtg cacagcaccg acaccttcaa
     4141 caagaaatac aggagctggg ggttcaccta tcccgccatc tatgccgatt tctcgaaggg
     4201 aattgtcgag gccgaaggca atttccatca tacgggcgag cccatcagtc tcaccgagtt
     4261 cggtcgcgac aagaccgaaa cgccgattgt gatcacgctg ccgcgcagtc ttcgctgcta
     4321 ctattggatc cgctacgtgc atcccggcgg caagcgcctg gccgatgcga tccaggtatc
     4381 cgaggacacc gttatcgaca taaacggaag gaaagtgccc gccacggcgt tcacctgggt
     4441 gaagctgaaa ccctgatacc tggcagcgcg agcgtcgccc cgtcgctcat atctcctcga
     4501 tatgaacaaa cccatctttc aggtaggcga tgataagccg tccgcgctcg tcgaagcgat
     4561 aggcggcgta ctgatacgga ccgtagcccg cttccttcgt gtgcagatag tagttgcgca
     4621 ggcgcatccc cggtcgcgcg ggatcgggat cgaagcgcac cgcgcgaatg cagtagcggc
     4681 cgtcgtcggg ggcgaagccg accgtcagga gggagcgtcg atcgacgtgc tgctcccggt
     4741 aatcgaaagg cccgtactcc gtttcgttca cgcgcagata ggtctgtccg cttttcgcgg
     4801 tatacgtaaa ggcgaggtgg gcgccccggg gcccgaagac gatatcgccc gcctcgagat
     4861 agggcccgta ccgccggccg tccgcatgca cgaaatggaa ttcgtccttg tacgcgaacg
     4921 cgacgcgcgc gccgtccgcc gacaggaccg gcacagacgt taggcgatag aacgagccca
     4981 ggcggcgggc gcccatctgg acaatgtccc ggtcgttctc gcggaacgag agcaggaact
     5041 gctttccgtt tttactcacg acaggcgcgt ttgcgtgcgg gaaatatccg aaggcgtgcg
     5101 gctgaaaggg cccgtatgac gcgccgttca ccatgacccg ctcggtgtcc cggtccccgc
     5161 gcatcgcata gcgaaaggcc acggtgtcgc ccgcggggct cacgcggaga ttcaccgcgt
     5221 gcttgtaggg gccgtaatcg cggtcagccg tcctcacgcc gcccatctcg ctgccatcct
     5281 cccgggacat acgatacaga aaggcgaaca cggtgcccgc atcattgaag cggagccagg
     5341 ggccctttcg gtggctgccc ggctccccga ccttgctaaa tgggccgtag gtgttcgcat
     5401 cgacgcgcac gaagaagcga tagggattgt tcgccccggc gccgtgggcg aacccgacat
     5461 gcgcgccgtc cgcgctcacg accggggcgg agacctcatc gggaacatgc ccctcgaagg
     5521 gaccgtgcac cgtcccgttc acgcgcacag agcgcctctt ttcgtcagaa tatgagaacg
     5581 cataggtcgc cccattacga ctcagggccg gtttgcttgc ccgattgaat gggccatagg
     5641 cggtatcgtt cacccgcacg aagtatatcg acctcttgta cccctcgcca tatttgaagg
     5701 cgtacagcga gccatcgtta ttcgcgacga aacccgactg cgagaaccac ggattcgagg
     5761 aagcaatatt cccaccatcg accagaaaac cgcgttgttc cgggggatcg gcgattcgct
     5821 tcacaaaatc atactcgaga agctttccaa tgggcggaat cagactcacg cagacaaagg
     5881 cgggatacac ggtacacttg ttttgaacgc agcgcttata catggacttt ccgcgctcgt
     5941 acgaaacgaa gaatcggtgg gcgccaaaga acgcgccgcg cgcgtgcgca tagggcccgt
     6001 acagacgatc gttcaccagc acatagcgcg aataatcaaa gccgtccttt ctttcctttt
     6061 gcagcatggc aaccataaga accgagctcc cgtcggggga aagctccagc cggtcccatt
     6121 catcgatgaa ggtttttcct ttgatgtcaa tcgacacagg gaactttttc acgggcgcac
     6181 gcccgcgcaa atctgcggcg ccgcagggcg tcatcatgac acgatacatc gtcagcacga
     6241 caacaggcat gatcagtgcg cggccccccg caatcatgag caacctcctc cagcacgccg
     6301 cgggcgcgcg catttcccgc acggactcac ccgcattata acgttgcacg acatcatggt
     6361 ccacgaccgc acgccccgcg ccgcgagcgc ctcatcgaag caattccgca tccagcggaa
     6421 gccaggtaaa cgcgcgacag gcgcgcttct ccgcgccgac gagtatcacc gcgtctcggt
     6481 cgaggagcag cccgtcgatc cggcgccgat cagcatcggc ataccggacc cagaagtcgg
     6541 gaatctcgcg cggtatcgtc actcgtttgc cggcccgatc cccgcgaagg cccaggatcg
     6601 aatggcagtt accgcggctc attccctcga tgatcccgtt ccggtaatcg cggaacatga
     6661 cgagggtccc attcgggatg tcgccgacgg gtttcccgcc caaatgaaac gtgcgcgtgc
     6721 cgcggattcc ttcaaccgaa atgctcatca cctcaccgct ttcgaaaaac acggcctcgg
     6781 cgggcgcgtc gatcgtctcc cggcggccgt tgatatccag cgtggaccgc tccctgatgg
     6841 cggcccgctt gaccgaaccg ctccgataga actcgatatt gccggttatg ccgatgggca
     6901 atcgtcgcgc tggcgagatc cccaccacga cctcatgata gccgcccaac tcaccggcat
     6961 gcacggagcc gtcgtcatgg aaagagacgg agccaaggcg cagcacctgc ccgcggacga
     7021 catagtcccg cacgctcccc gatccggccg atttgagcgc gccattctcg tggagccgga
     7081 cgatatcgcc ggtcgcgacg gcgacgacct cctttccgaa acgaagcgtc gacggagcgg
     7141 ccgccacgcc gcttttcatc ctgccattgt catgaaatac aacatatcgc ataaaggaaa
     7201 cgagccttcc ggcgcagggc gcctgaatgg ttaccccgga cttgagatag actcccgaca
     7261 gccgtccatc ctcccgaaaa gaggccgcgg cgcctctagg aaagcgcagc tctccgccgt
     7321 cgaccggcag cgtttggccg gccgccggat atccggcgcg aactacgccg gactcataaa
     7381 attcgatggt gccgtaaaag cagtatcccg gtgatttgtc cgaggcgcac ccggatgatt
     7441 ccggatcgtg aaaaccgagg ccgtcctttt cggtgggctc aaagagcacc cgccgctccc
     7501 gaacggtaaa tgcggtgtcc cgctgcaaca cgcactccct gagggttcca ttctcgtaaa
     7561 aggcggtcgg tcccgccttc acggaaaccg gcccaagggc cgtccgggtt tccccgccgt
     7621 cggcaagatt ccccgtcttc acccggccgc tttcgtggaa cgtggtccat cccggactga
     7681 agcggaggcg cttcccgccg acgttcaaca tggcctcccg cgcgagattg cccgtgcgga
     7741 gacccgtccc gtcgcccgtg ttccatgcga cgaggccctc cgttctgaag acggcctcgc
     7801 atcccccaac ggtcatgcgg accgtcgtgt gttcgcgata ggacttcgat atcgcatccg
     7861 ccagcgcggt gaacggccac aggagcacat acaggcaaaa cgaaggcccc ttgggcacgg
     7921 gtggatccgc atgggcgaag gctccgctca cggagaaaac ggcgacgagg agcgcgatga
     7981 cgatcgtgcg gcatgttttt ttcgaggacg ggcccatcgc acacgactat caccatgccg
     8041 gcggcatcgc aagcacaaat ccgcacgggc ccttcacgct tcacgcaacc caggccatgg
     8101 cgcatgatcc ggagcgcgct ccggccgaac gccgaacgcg cccgcggtcg cttgcgcgca
     8161 acgtgaaccg accgggggag cacgcgacgc gcatcataca taaaaaggtg attgacagaa
     8221 ccgccgccgc gcccatacat gagggaccgc gcgacaaaga aaatccaggc catatcggac
     8281 tatcggcgcg gacaacacca tactacggtt tcaggagtat caacatgaaa agactcatgg
     8341 gaatcatcgt cgtgctgttc ttcatcgcat ccctgccgag ctgcagtgta tataataagc
     8401 ttcggggata cgtgggcatc ggcgacctta agcgggtcgc gaacctcatc agcatggact
     8461 tcggcaaggg tccggacggc cccttccaag tgagcaccat cgcgtcggtc gatccgaagc
     8521 tcgtcgtgaa aaacagcacc gaccgcacca tcaccgtcaa ggcccagggc cccacggcca
     8581 agaccttcgt gatcaattcg ggccagcagt cggaggggac agtgaagagc ggcatgtacc
     8641 acttcgtggc ctccgcgccc ggcgtctcga gcgccgaggg cgatgttgag ctcaagggtt
     8701 tcaagcaata caagtgggag ttcatcattc gcaaacagta atgcgaaccg cgcgcacggg
     8761 cgtcgtttcc ggcaggaccg tccgtgcgcg tgcgacgttc atcggcgcgc tcatcttttc
     8821 cccgacatgt acggccgtcc cctacatccc cctggaggtc actcgaccct tttcacgtga
     8881 acgaattcct tttgcgacga atgatccagg gttttggatt acgctttcga cgaaatcccg
     8941 ttcgttcatg tgcacgcatc actcgatccc ggtgccgacc gacaacggga atgatcaccc
     9001 gccttcagta aaatccttgc cgccaatccc cggttcgcga tagcgccggt agtaatgcgt
     9061 gagcgtgaga tacgtaaaca ggcggttcac aagcggtatc cggaccagat aatagaacag
     9121 cacatacaca agaaaaaacg agacgagcac atacgcgtag ttgaccgcgg accgcacgaa
     9181 cgcgccgccg ggacccgcac cgatcccgct caatcccggc tggagccgaa tgatccaggc
     9241 gccgagcgcg gccgccagcg aaaacgttcc gacataaaac atcgcgattg cgagcggatg
     9301 gctcgcctcc acggcctttt ccggacagta gcccatgcag tgcatgcagc tctcgcaggc
     9361 gaaggtccaa tacggcagct ttgggtccgt tcccatcatc cggatggccc cgaaggggca
     9421 ctcgcgggcg cacaatccgc atgacgtgca tttccagttc gcatagaaga gcttcgcgag
     9481 ggcgaaacgt ccgatgagga gatagcccgc ggaaatattc gcaagcgcga tcccgccgac
     9541 aagctcgacg aaattattga tgctgacaaa gtgagttttc ccggagaaaa tgagcgccgc
     9601 gaggcgttcg atgtcgatcc tcgcgcgatc gatgatccgc tgcgcgtttt tcggggacat
     9661 gccccagtgg agcgccgtcc agttcgacgg catgtcgaga ccccgcagcc ccttgatccg
     9721 atatcccttg agcatcaaca ggaacgcggt gacgaacatc gcgctcgccg aaagccccgg
     9781 cagcaggaac cccagccagg tccccgcgcg ggtggccacg acaaaggcgt cgctccccgt
     9841 tccgcgcggg atccgcatcg catgtcggat aacggcccac ggcgcggtaa agccgtgcgt
     9901 cgggaagaca aatccggtca tagagtttcg atccggcgcg ggcggggcat catacgattt
     9961 cgcgatccgc acgatcgtcg cgcccccggg accgaacaga tcggcaagcc agcgggccgc
    10021 gcgcagcgaa tttcccgtgc cgctcatgac gtgcaggatg agggaatcat acgatcgcgt
    10081 catcatcatc ctcccctatt gccggatttc gatgaccggc atcgccattt cacgacggcg
    10141 cgtttaacat gagcttgtcc tgaccgcata caggacccac ttccccttct gcaacctgaa
    10201 atacaggttg aaccgggtgg cctcatatcc gtaaaacagg tgcacgcttc ccttctcgta
    10261 cgtgaactcc tgcttgtaca tgaagggtct cttcatgtag aggtcgttca gctcgagggt
    10321 ggcggcggtc tttgcgctgt cggtgcagtt tccctcgaag caggagacga ccgtaagcgg
    10381 gaagaccgtc cgcgacctga tatagttcag atctcccttt tcgttcgcgg cggcaaacgc
    10441 ctggaagaat acgtgaaact gctcgtcggc atccccgtcc tcgcggacaa agctgctgaa
    10501 cacgtacccc tcgcgggatc cgctcttgat gcggcaccac cggcccagat acatcccggc
    10561 aaatcccatg ctgcccgttt tctcgagcac cgtcacccga tcgccggatt gcagcttccc
    10621 gacgacggcg ctcgcggcat ccggcttcgc gcggatattg acatcggagc ccgagatcac
    10681 cgccgcgaat gcgggagatt gatatgttgc cagcagaacg ataccgcaca gaattaatgc
    10741 cctcagcttc atgtcatcac ctcgtgtatt gttgcggatg ttcgttccgc ccgcagggcc
    10801 gcaagactga ccggttcgca tccgcgacga atcgccatcg ggcggcggtc gatcaaaaca
    10861 cgctcatctc tgtttccgca gagccgtaac accgttcgcg tctgagacgg aacgatccat
    10921 atcatctcac agtcgcttca ccgcgattcg ccggtcgcac gcgcccggct tctcccggta
    10981 gcagaacgcc gcgagcgcgc cgtccgacga aacgacgatg gaatcgatca ccgcgaagtc
    11041 ggtcgcgcgc agcaggcggc ccgctgcgta ttcgtagtac ccagcgtgcg ccgggcttcc
    11101 ggagatgcgc aacagcgcgc ggccgcggat catccccgtc tcgcgcaggt acggatactg
    11161 ttcatttaca tcgagcgcaa ggaggaccga gggcgttttc ccctctcggt aacggagaac
    11221 atagaagcgt tcattccaaa accaggcctc gttcagggcg aggatgaaat catcccccat
    11281 gcgagcggcc ccgttgagtg cgccgtcatg gaaatcggga acggggcgga taacccgcac
    11341 ggcctttttc gaaaaatcga gcatgcgcaa aacgctccgt gatccattgt ccgtgaaatc
    11401 ctggacgata aaggcccccg tcaccagcgc cctgttcctg ctttcaaagg ccagcggagc
    11461 gtcggcgggc tgatagggcg cgagcctcgc ctcgagctcg cggcgcgacc gttcataacc
    11521 gcgctctcct ccgccgtcca cctcgtgctt caggatacgg cacgacagcg cgtccaccgt
    11581 gagctcgata ttgtccacat ctccgtcgtg aagcgtgacc gtgaggcgat cgtttgagcg
    11641 ccattcgatg ccgatgatgg gcgccgcgta cacacggtat ttcgcgcagg cgtccgcctg
    11701 caggcagagc gtcggctgcc aggtcgatgt atccagaagg atgagctcgc cggtcacgaa
    11761 gtcgtgcgga tcgtttttgt ttttcgaata ttcggtcacg cccctgcgaa tggcctttct
    11821 cgcgcaggcg agccgccgtc cgttcgggga gaagacgggc aggaaataat cgccgtcggt
    11881 gagcgcggcg atgactgatc ccgatgcgat cgcggcgtgc ccgggcggga ccgccggaag
    11941 agactgaaag tattttctcg ccggctggta gtagtggcgc accttgctcc ggagcatttc
    12001 ccggatcgcc gggttgtccg tgtatccggc atactggtgc atgagccgcg ggaagttctt
    12061 cgccggcatt cccggctccg gcgcgcccgg gaccctcatc gacgcggcgg ccttcgcgaa
    12121 ctcgcgcgca ttgcccttga tgagcggaag caggatcgat tcgtactcgg gattccaatc
    12181 ccgctcgttg gctacgcgtt tcaggagctc tccatagggt ttgagcgtcg cctcatgcat
    12241 ccagcccgtg tgtcccgacg cagttttcac cgtgatccag tgccgatcga acctttgatg
    12301 ccgcagctcc acgttcgtcg tgtgcttcga gcgttcgccg gtgaactcga cgtcctcgcc
    12361 ctggtcaagc caggccacca ccggcgcatc gagcgacggg gcctcgcgca gcgcggcctt
    12421 gtcgacccag gtatagtagc ggttctccgc attgcggcag gagaccagtg caagcgcaag
    12481 catcaacgcg acggacagtt tctcgtatga catggcggcc tattgttttt tccgcacggg
    12541 tgcggggtga tgattcgttt ttcgcatgtc gaccgtgtag ctgtcaacga atttcaacgg
    12601 gcaggtgcgc cggaccctcc gcatgcgcgc ataaaacagc cccgcgcctt gaagggcgcg
    12661 gggctgtgca gaaaacagac attttcgata tcgcgctacc ggtatcgacg ccgcccccga
    12721 ttggcattgt tcgaccgctg cggcggccgg ctgccgctcg cgccgggacg tttcacggcc
    12781 tttgcggcag ggaccggaac gtgcgcgccg acctccagcc gatagggatg ctcctcgacc
    12841 gccgcgatgg ggcgcgcgag aagcttcgtg atgtccgtga ggtacggccg ctcctcgaca
    12901 tcgcagaacg agagcgcgat gccggagagc cccgcccggc ccgtgcgccc gatgcggtgc
    12961 acgtaggtct cgggctcgtt ggggagctcg aagttgatca cgtgggacag cccctccacg
    13021 tcgatgcccc gcgacgcgat gtccgtggcc acgagcacgc gcgttttgcg cgccttgaag
    13081 tttccgagcg cgcgctggcg cgccccctgc gacttgttgc cgtggatcgc ctcggcgcgg
    13141 atgcccgcct tctcgagatc gcgcgcgacc ttgtcggcgc cgtgcttggt gcgcgtgaag
    13201 acgagcgcgg cgtcgatcga cgtgtccttg agcacgtgat gcaggagcgc cttcttgtcg
    13261 gccttcgcga catgatagat ccgctgctcg acggtgtcca ccgccgagga gacgggcgtc
    13321 accgcgatcg ggaccggctc cgtgaggatg tcgtcggcca gcttccggat ctccgccggc
    13381 atggtggccg agaaaaagag cgtctgccgc tcgcgcggca tcttcgcgat caccttccgc
    13441 acgtcgttga taaagcccat gtccagcatg cggtccgcct cgtcgaggac aaacgaggtc
    13501 acggcctgca ggctcaccag ccgctggctc atgagatcca gcaggcggcc cggcgtcgcg
    13561 atgaggatat cgaccccggc cctgagcgcg tcggtttgcg gcttctgcga caccccgccg
    13621 tagatcgcgg catgccgaag tccggtgtga cgcccgtagg cggcgaagct ctccgcgatc
    13681 tggatgcaga gctcgcgcgt tggcgtgagc accagcgtcc gcagcgtccg gggacgcgcg
    13741 ccggcgctgt tcgcatgcca cagattttgc aaaatcggca gcgcgaacgc ggccgtcttc
    13801 ccggtgcccg tctgggcgca gcccaggata tcactcccct gtaaaatata cgggatcgcc
    13861 cgctcctgaa tgggcgtggg gacctcatac ccctcggtct tcagggcccg catgaggggc
    13921 ccgatgaggt tcaactcttc aaacgatgtc actgattgtt tctccttgcg atcgtcatga
    13981 tcgcgtgtaa ttgtcggacg agtgcgcgtc cacgcatcag cagttccagg tgaaccactc
    14041 gtatcgtccc gaggcgacgg catcgcgcgc gtcctcgggc tcatagcgga acagacggcc
    14101 cttccggtca cgaacgagcc cggccgcgat aaagctctcc accacctcga ttccccgcag
    14161 gagctcctcc tttacgtgac cgtgctgctt cccggatgag agcgtcttgt tgcggacgat
    14221 ctgagcgaac tcctgtttcg tcgtcaggaa ctcgtggtcg gttttgcttt ttcgtttgtc
    14281 ggtgatgatg ttttgccgaa gcatacttcc ccccttggtg cgcaccacga tagtgcaatt
    14341 caacgacatg cgccacccgt ggtccgcacg ccatacagtg cggccagtgg caacgaaacg
    14401 gcgccggtgc gcggttgcgt tcgatgattg acgttggcgg acgatgcgag cggaacgtca
    14461 ggggcagtac atcagacggg tttccacgaa agggaacgaa tcacgcgccg gtgagagtgg
    14521 gccgcgattc aacgtgctgt taccggaaca ctgctgttat tgggcactat ccaatccccg
    14581 tgataattgt acagcatatt atgcgaatca ccacaaaaaa gtcgcgcgtg ccgatccgct
    14641 agcgctccgg cagggcgcac tcagggctgt agagcatcac gtctcgcacc gtgtcgcccg
    14701 aggcgtcggc caggagatcg cggctcttcg ccatgcactc gcgcttcaag ccccccttct
    14761 cgtcgagata aaagagcatg ctgagcacgt acccgtcgat gaattccttc tgctcctccc
    14821 gcgggagccg ggcgagcttc tcgagaaatt ccgggtactg ctgggtgatg acgaggtcca
    14881 cgccctcgtc gcgcctgagc ttggcggcgg cgagatcgcg cgacagcgtc tcgatgagct
    14941 tcgtgtcgcc cgcctgcgtg ctgctgagct gaaagtaggc ccagcccgcg cgcagcatgc
    15001 tcgtgccgtc gccatgcggc gtcgagcggc gcggatcctt cgccgccacg cgcggcatgt
    15061 ccggcgccgc ggacggggcc gcagcgccgc gacgggacgc gacccgcgcc gtcttcgggg
    15121 cattgcccgc atcgatggca accgcgggcg cactgctcgc gagatcgatt ccctcctgcg
    15181 gcgtatcatt gattatattg atcgcgacaa cgctcgccat gatgaccagc gcggcggccg
    15241 ccaacggcaa cgcgaaccgc aggggaatga tccttcgcga tgcgcgcgcg gcggcgaagg
    15301 cgcgctgttc gagatcgggc gggacgcggt gtcccgggag cgcggcaagg cgccctacct
    15361 cgatcgcggc ggagcggcac tgcgcgcaat ccgccatgtg ctcgatcgcc gcccgccgcc
    15421 ggtcggcggg aagcgttttg tccacaaacc gcgcgatgtc cgcatcgccc aggcagcggc
    15481 cgggtccgtt ctttcgcttc atcatcacaa tccctcctcc ctgagcagct cctcgaaccg
    15541 catccgtgtt cccgcgtcga agctcagtcg cagctcctga tggatcttcg tgaggcggta
    15601 ccgggcattg tcgatgccca ggtgctcgtc gatctcgcgg tacgacatgc cgtactccat
    15661 gcgcagcgcg acgatgagcc ggtcctccgc gtcgagccgt gcgagcgcgc cacggagcgc
    15721 gtcgagcgca tcgcgcatgg cctccgcgcg ctgcgattca agcacctgcg cctccggaga
    15781 gcgggactgc ccccccgagc ccagcgcctc atcgacggcc tccggcacga tgaactcgtc
    15841 gaattcgcgc agccggtctc gcgtgcgccg ctcgcgacgg atgtaatcgg tcgccaggtt
    15901 ccggacgatc gtcgccaggt aggtccggaa ggagctcccg ccgcggaact gccgcagctt
    15961 ccggcagccg ttgtcgcaca cccggacgat gatgtcggcg aaacagtcgt cggcgtcctc
    16021 ccggggcagg cgcgccgtcc atatcttcgc ccgcgcgatg gcgtagatga actcgcggta
    16081 ctcgcggaac aggtgctcca tgagggcgtc atcgtcgagc gcccgcagcg cgcgcgcctc
    16141 gccgcgcggg tcgtcgaaac cggacgtatc gcccgccccc gccttcaccg ggcccgttcc
    16201 attttcggaa aatacatcat cgaacaatcc gtcggtaagt atacaggcca acggcgccgc
    16261 atcaaccctt ttcatcgcca tcgcgcacct ttttcgaacg tcgtatcgaa atcgatctcg
    16321 catagtagac gacgccggca tgcggatggg aaaaaaattt tcgttatggc ggttgattat
    16381 gtctcattgc ccggacaggc gctatgccgc agaggagggg ccgtacgact accgcgcctc
    16441 ctcaaggaac aagcgcccct tcgccaccca ggccgtgaag atgcggtttt cgcgtatgac
    16501 aaaatcggcg gcgtcatacg ggccgcgcga tataccgtcc accatgaggt acacccgttt
    16561 tttggcttcg aagacgcaac aggaccgcgt gccgtcggcg gtgaagccga tgcggatagg
    16621 gcgatcgcgt atttcatact ctcgatcatc atgacgcagc acatatcgaa ggccccggct
    16681 cagcgaaccg acctggaaat aaaaaaggag atgcttcccg gaatcgtcga ggtgggggcc
    16741 gaggacgaaa tcgtacgggc catattcccg gttattgaaa tgcacgaact ttttgtcgct
    16801 tcgctccaac acgaaggcga tgctgtttcc cgcgcggttg atcgcgagac cgctggcgga
    16861 atcgaaaccg ctcagacggc ggtcgttgaa ctgaacaaag gcgctgtacc ggcccgcgac
    16921 atccccaggt ccggtcgaaa ccatgacgtt caaccatttg aagctgcagc aatgatccgc
    16981 atgccgcccc aacaagacca cgggcgcgca gccgtaattg tagcaggtgt cgatcggtcc
    17041 cgcccgcctg ccgataaagg cgaaacttcc tccagtgtcg ctcagtgtaa gccgatcaat
    17101 cgacctgaag ggacccgcga tccgccggtc gccgatgaac agttcctgat ttcgattgtc
    17161 gcgcgacacc agatagtatc gcgagccgtc agggctgaag acggcgtccc gctgcgtatc
    17221 gaacgggccc agcgtcatcc cgtcgaccac gcaatagtat gcgctcccct ccagatagct
    17281 gaaggcatac cggccgcccc gatccgagaa gaccggttta tcaaaaacaa tttcgtacgg
    17341 cccgaacgat cgcccattga tgatgaccct gttccgggga tctggttttt ccaccgcgcg
    17401 cggattgttg cggaggattg gcgcgcgctc ccgctgcggc gcggcgatct cccgtgcgac
    17461 aacaccgaac aggctcccct ccggattgaa tccgtaccat gtgacctcgt cgaacggccc
    17521 ccggcaccgg tctgtgtcgc agagaaatga gcgatcgctc cccggcatcg gcttcttgta
    17581 gagatagttc gatccgtccc ggttgatcct gatcccggag gcgccgacga ttcttcgcgc
    17641 gttcccgcgg ccgtccacga tcacggcgct ttttccgcgc ttgtccgatc gatcgtacgc
    17701 catgatgcca gtggcgctga agaccgggct ccccgcccat tcggcatcga tatcgcggta
    17761 gtctccgttc agctgcacgg cgtaaccggt gttcacatac ccggcgctgg cgagatggac
    17821 cagcgccgct cgcgacccgt cgggcgagat gaccaggtgg gtcttctgcg ggtcgctctt
    17881 tatatgcacc gggaagtccc gcgcggagct ctcgcgcggc cagcacagga ggaggcacag
    17941 cataacggca atacggcatt gtgtacgcat gatgatgggg ccgccgctca ttcgaccgca
    18001 tgccctcata ttcagccccc cgcctcgata cagacgaggc agaggtcgtc atcgaactca
    18061 tcgcccccat gaaagcgcgt cacctcatcg acgatcgcgc ccgcaaaggc gtcggccggc
    18121 aacgccgcgt gtttcgccat gacctcggcg agcgcccgtg cgccgaagaa ctcctctccg
    18181 ctctgcggcc ccaggacctc gagcaatccg tccgtacaca gaacgatctt gtcccccggt
    18241 tcgcagcgga cggtctcatt gacgtacggg ttgtacttcg cgcggatctc atcattgctc
    18301 acgatgccga cggggacgcc gatactctca gtggacagag aatctgcgcg cgcgtcatgg
    18361 acatggaacg gggagacatg tccggcgctc gaatacacga aggtattgtc gcgtcggtcg
    18421 aagatcccgt agagcgcggt cacgaacatc gccccggtgt tcccgaagag cgtcttgttg
    18481 aggccgaaga gaaactgcgc cgggtccgtc cgctcgtcgc cggtcttcag cacgacgctt
    18541 ttcaccatgg aagcgatgag cgccgacggg acgccgtggc cgcacacgtc gctgatgaag
    18601 atcccgatcc ggtccgcctc ccgaaagcgg atgaaatcga agtaatcgcc gcccaccttc
    18661 atcatcggca tgtagcggaa cgcgatgcgc tcccacggcg gacccacggg gatgagcttg
    18721 cgctgcacct cgcgcgccat gtccagctcg cggttcatga tgaaattccg cacctcgagc
    18781 ttctcgcgct cctcgaagta ggaatcgacg acaaacttga agatgaagcc gacgacgacg
    18841 atccacgtca catagttcat gaggtaatcg gccacgcgat cggcctccga cgcatacgac
    18901 ttcacgctgc cgggattgag gtgctcgcac agcgtgagcc ccgcgacaac gacgggcgtc
    18961 ataaaaaaga agagatagcg caccgccccc ttgaggagga tcagcgagaa tatcgcgcaa
    19021 aaaagaaaaa acagcggatc gccgccgcgg gagccgtatc ccgtgaacca gatgaccgag
    19081 aggagcccga acaaaagcag aatgatggtc tttatcagcc gaatccgcct gaagcgggac
    19141 aggtagtaga tgccgatccc gcacgcgccg atgaacacga cgatgaggcg atgcgcaaga
    19201 ccgaagtcca ataacaacgt gccgatggtg atgatcgggg cgcatatcgt cgccaagagc
    19261 agcatcgcat tgaggatctg atgctccaga acgaactcat ctttcccgcc cacaagatag
    19321 gttatgagcc gtttgattgt attcatcgca ccgtgtagcc gtactcagag cgttcgcgcg
    19381 acgcagcaca catcgccaaa atcaaggaca acgggcatca tggcgtttta cccaccctgc
    19441 gatagcgccc gtcgaaaaag acgcgcgcgc cgtggtgata agcgcaggcg tgctcccgca
    19501 cgtcgatcca gtgcttgccg aaggcgaact cgatacggca gcatccgtcg cccgcgcacg
    19561 gctccttttc gcggaactcc gccgtcccgc cgcggaccgc gatgtccccc gatatcgatc
    19621 cgagatggaa ggtcggtccg cggacaacct cgatcgtaaa atgaacgctc ccgtccgtat
    19681 gtgtccgcag cgtcatgacg ccgccgaacc cgtcgccgta cgcgccatcc acgcccgacg
    19741 gcgccgaagt cttcccgaac gcggcgcgga tatacgcggt cctgtcacgc gtgagatcgc
    19801 gcaggcattg ataatactca tcgtcccgcg cgtcatcctt ccccaaccgc atggcggcct
    19861 gatacccgca cagatagtcc ctgtggcgta tccactcccg cgagttctcc cgcagctcac
    19921 gcttcatggc ggcgtcgagg cggcgcatga tcgcgcgaaa cgcctcgttg agcgcgcggt
    19981 cctctttctc gtatgccggt ttcgacggaa acgacggcgc cgcgcccaag ggcgtcccct
    20041 gcccgagcat gcccggcgcc gcaatggcac aggcgagcgc gaccgccggc gggatcaggc
    20101 gggatgtgat tgtgatgatg ttcgccatga ctatttgtat gcgcatccct gaaaaatgtc
    20161 aatgcatttg cgagggggcc gatacgccga agcgttcgac ggcgtcattt ttcgccgtca
    20221 ggggcgcaat aatcgttgtg cgaatgcgcg ccttcgggca taccggtcgg cgcacacgca
    20281 cggaatcatg caaacgccat gaacatcacc gacttacttc gccaagcctc ccaacggctc
    20341 caggaggccg gcgtcgaaag cccgcggctc gacgccgagg tcctgctcgc acatgcgatg
    20401 ggcatcgacc gcgtgcgcat catcaccggc ggcgaccgcg aggtgcccga taacgagcgg
    20461 cggtcctttg acagcatgat cgatcgccgc gcgcggcgcg aaccggcggc ctacatcatg
    20521 ggcgcgaagg aattctattc gctggaattc gaggtgaacg agcgcgtcct catcccgcgc
    20581 ccggagacgg agcttctggt ggacctcgcg ctctaccacg ccccgcgcgg cgggcgggtg
    20641 atcgacgtgg gcacggggag cggcgcgatc gccgttgccg ttgtgcgcaa tcgtccggac
    20701 atcgccatga ccgcgaccga cctctccggc gacgccctcg cgctcgcgca ccggaacgcg
    20761 gacgcgctgc tcggaacgca cgcgatacgc ttcctgcagg gcgatctctt cgcccccgtc
    20821 gcaggcgagc gtttcgacct catcgtatca aatccgccct atgtcgatcc cgcgctgaag
    20881 ggcgcgctgc agcccgacct cgccttcgag ccggagttgg cgctctatgc cggcgaatcc
    20941 ggcacggcgg tcgtcgcgcg catcatccgc gacgcccgcg atcatctcgc cgacggcggc
    21001 atggcgctca tggagatctc ggaaacgatg aaggacttcg tggagcgcga ggcgtccgca
    21061 tgcggcttcg cggtgtcggt cctgagcgac tatgccggcc tcccgcgcgt cgcgtcgctg
    21121 acgacgcgcg gcagcgcgca cggctgatcc ccgccgacga tttcattcac tattcatctt
    21181 cgaagttatt ccatatctct tcggtgaatc ccaaccccat ggcggcgttc cgctctctat
    21241 cgcttaaatc gtcccattcc ttatcggtgc tttcaggagc ggtattacca tcccacagtt
    21301 cttcactcca tccgagagcg ctccatagtt ctttctctgt atcgtttaaa tcctcccacg
    21361 aaagttcttc ccacatcccc ggatttccat tcatatcgcg aacttccata acaacctccg
    21421 tagaagcgcc gttgaagcgg gcgctaaggg cgcacaggat gaattctttt aaccagatga
    21481 tagagcacgg gaagcacgac gtcaagggtt tttcaccgca tggaaatccg gacttgacga
    21541 aatgtcgctt gacattatac ttgatatatc aaatattgac atatcaatta cttaccgggg
    21601 ccccatggac gaccgattgc tctttctgat gtcgcgcgcg caccatgcga tcaagaacca
    21661 cgtggcgggg gaattcgccc gcgaggggat cggcatcacg ccggcgcaga tggggatcct
    21721 cttttcgctg agaatgagcg acggcctgcc gatgagcgag ctcggtcgca tcatccgcat
    21781 cgataatgcc gcggtcacgc gccacgtcga cagcctggag cgcgcgggtt gcgtgaggcg
    21841 cgaggcggct ccgggcgatc ggcgcaagag catcatccgc atcaccgcga agggacgcga
    21901 cgaggcggtg cgctgcgcgc ggaccgcccg gcggatcaac gccatgctca aagagggatt
    21961 caccgccgag gagatcgcga cgtttacgcg cgtgctcaac ggcgttattg aaaaatgttc
    22021 acccgccaat ggaggtgtat gatgagtcag gtattgcgta tttacaacgc cctcgcgggc
    22081 aagcccttcg ggaaatggct tttttcgcgg attctgtgct tcaaggcgcc gtatttccgc
    22141 accatcaagc cgcgcttcgc ggcgctccgg ccgggttatg gggagatccg catgcgcaac
    22201 cgccgagcgg tgcagaacca cctgaagagc gtgcacgcca tcgccatgtg caatatgtgc
    22261 gaactggtcg gcggcaccac gctcgaggtt tccatcccgg cccacctgcg ctggatccct
    22321 cgcggcatga cggtgcagta tcgaacgatc gcgcgaagcg acctcacggg cacatgcgcc
    22381 atacccgacc acggcgcgat cacgcccggc acgctgccgg tatcggtgag catcacggac
    22441 acacgcggca cggaggtctt ttcggcggtc atcgacatgc atatcagcga acggaagcgg
    22501 acgtgaggcg atattgatca atcgagccga atgacactct atgcgcgccg agcggaattg
    22561 gacgcgcagg aataacactt gacaggcgcc acaatagtat taccattcgc atgtattacc
    22621 attaccggag atgagacgcc ccatgaagat caccacgaag ggacaagtca cgattcccca
    22681 gcacattcga gagaagcacg ggctcatgcc gcataccgag atcgacatca ttgaggaggg
    22741 cgcgaaaatc gtcattgttc ggtcaaagaa gtccaggcac tccgaccaat tctctcgatt
    22801 gcgcggaacg gcgactgtac ggatgtccac ggacgaaatc ttacggctca cccgtggcca
    22861 ggaatgaagg gccttctcgt cgattcatcc gttgtactcg acatcattac tgacgatccg
    22921 acctgggcgg actggtcaca gcagatgctt gcacggcacg tgacctcgca tcgcctgtac
    22981 atcaatccaa taatttacac ggaggtttcg attggttttg aacgaatcga agaagttgaa
    23041 ttcgttattt ccaattcggg actgaaaatc ctccagatac cgcgcgaggc cctttttctg
    23101 gccggaaagg cattcctgcg ctacaagaag cacaagggct cgaaatcatc cacgctgcct
    23161 gattttttca tcggcgctca cgcagcggtt tcacaactcg cggtactcac ccgtgacgta
    23221 acgcgaatac ggacctactt ccccacggtg cgcataatcg ccccctaaac gaacgcatcg
    23281 ctcactgcgc catcttcacc gtcgcgatgt cgtgcatgtc ctttccgtac tcgagcgtga
    23341 ccgtcttctt ttcgatcgtc tcgcgcttcg tgccccagta acgatacacc gtcgcgtaga
    23401 ccttggtgcg cgcgcttttc cggttgctgt ccgacgcgaa gtacttcacc ttcacggtga
    23461 aatttccggg cacggatttt ttcaggagat acatctccgg gccatagccc tgcgtcacgt
    23521 cataggtgag ctcgccgccg atcttcgtgt tcttgtggct gtagtagcac tcctcgccgg
    23581 agggctcgta gacgtgcagg tccacgtcgg tgcggtcggt gttccacatg atggtgacca
    23641 tgaggtccgc gcgcgagatg ccgatctcct tcgtgagcgt cttcaatcgc gccatcgcga
    23701 attccctgtc aatcgtccgc gcggtcccct tcctgatctt ttttaaaaga ttgagatagt
    23761 cgagcatgag gatcttccga aaatcgccga accggctgtc ccactgcccc gccagcccca
    23821 cctcgtagta ggcgattgcg aggtccgtgc ggcccatctc cgaaagcaac agcgcgaggg
    23881 catgataggt atgcggctcg aacggacgcg cctccgccac gcggcggaaa aggtggtagg
    23941 cgtggtcgcc cagcccccac tgcatcgccg tgaagccgac gtcgcgcatc agcacgccgt
    24001 cgccggggct catctccacg agcgagctta cggccttgag cgcgtccgcg gcgccgtgct
    24061 tcgcctttcg gcggttcgcc tccgcggtga acgcatcgta ctcgatgtcc ttgttgcgta
    24121 tttttccgcg aaccgcgagc ggcaattgct cccagcgaaa gaccgagcag gcgagcggct
    24181 tcggcgttat ggcgaacgac gaatccggca tttccttgag aaaatcggaa agcgcggtgg
    24241 gaacggaaaa tttcacaccg ggcatctttt ccattttcgc aagccacgcc aggaagccct
    24301 ttttcgggtt ggagagggaa tcgccgatcc gctgcatgac gcgcgcgatg acgggggccg
    24361 ccgggttctt cgcgaccacc agcgcgtcct cgtcgggttt gatattgaag cgccggtact
    24421 cggcctcgct ctccagcatg agaagggagc aggtgttgcc cgttatgcga tagtgcgtcg
    24481 cataggcgcg cgagtatttc tccacggcgt cggcgaactc ctcgagctgg cccaccgcca
    24541 cctgcccgta gacgcgcgcc gcgagctcgg tgcgcaccgc gtgcctgatg ggcgcggcca
    24601 cctgcagacg ctttccggcg gcgtcgcgca gcgacagcac cacgcgcgcg ttggccggaa
    24661 tctccttcgc cgcgcgcccc gccaggagga gggcctggcc cgggtacacc gacaccggcc
    24721 gcccggccag gagcaggtcc gtgccgccct cgagcgtcat ccccacgagg cgccaggagg
    24781 acatgcgatg cgcggtggac gcgcccgcga tctcgttctc gccaaccacc gaaaagaccg
    24841 cgccgcccgt ctcccgcgcg atctgcccca gaagccccat atcgctcccg gccagcccgg
    24901 tgcgataggc gaacaggcgg ccgcggccct tcagcgcgag gacgcgcgag agcgcggcgg
    24961 cattcccgtc gccccacgtg atcgcgccgt cgctcagcag gaacagggtc cagggcgcgc
    25021 catcatcacg gcgcagccag gcgggactca cggcctccgc aagcgcgctc ttgatgtcgg
    25081 tggcgccctc cagcgcgagc gcgttcatgg cggagagaag ctcggcggtc gcgtcgggcg
    25141 tgtttttcac gaaagcgcgc ttccaccacc gctgttcgat gttgaagaac aggaccgcga
    25201 actccgggat ggacgcgcgg ttgttcgcaa ggaccgcctc gacgagctta cgccatacgt
    25261 tgaactggtc cgggttcgag ctcaacgacg tgtcgaccag gaagaccgcc ttctgggccg
    25321 cggttgtatc cgacttcttg ggaagcggcg gccggaacat cgtcgcgaaa tgcacgccga
    25381 ccttcccgtc cgcgccgtgc aggaggaccg acccggggtt cttgaggcgg acggtcacgg
    25441 tttccttttt tcggttcgtg aagcggtaca ccgtggcgtc acccgccttg tacgggctcg
    25501 tcttcagatc gacctccgcc gtcacgttcg gcaggtcgcg cacggacacg tccaccatcg
    25561 attcgggaac gtccgggatg tcgaagcggt actcgaggtc gtcgcccgcg gggagcaggt
    25621 tcacgtcgta gccgatcacc acgcggtgaa cggtcttcgg cgcgagcgga aacacccgcg
    25681 cgctgaagat ccccgcgccc gaccactcca tgagcgcggg atcgacgctg cgccgcacgg
    25741 tctcctggta ggcaaaggcc gccttctcct tcgggaccat gcgcgcctcc ttcggctgct
    25801 tccactgcgc ctgccggtcc tgcatgatct gctcgggctc catgcccatc ttctcgccgt
    25861 cgggcaggct gtagaacatc tggcctttcg ggatgtcctc ggcggcgtag gcggtctcgc
    25921 cgaaggcgaa gaaatacggc gacgcatcgt cggggagacg cagcttgaag gtcccttcga
    25981 actgccgcgc atgctcgtta tggaaatagc agtcgatgag aacgcgcgcg cgaaagccga
    26041 cgatccgcac cttcgcctgc atggccacga gccgcagctc ctccttgtcg ccgatcatca
    26101 gccgcgaagt gttgggggcg atctcggagc gcttccacgt cttgacgtcc gatcctttcc
    26161 ggccgcccgc gtcctttttc gccatggcct tgtagcggcc ccgcggcgcc tcgtccgcca
    26221 tggccttgtc cagatccatg cccgcctcct tttcggcggc ccgcatcgtc tccttttttt
    26281 ccatggcgct ggccggcttc tgatccgagc gcaggtcgtc ggatgcccgt ggcgtggcgg
    26341 tcttctgtgc gctgcagccg gtgagtgcga aaaacagcag cgcgacgaca agcaggcgag
    26401 gcgtttggat cttcatggcg ttctcccgtg taatggaatg gcatggtgta tcccgacgag
    26461 gatgaaatgt cgtaactatt cagcactttg aggcataaat ggcctgtttt caaacaaccc
    26521 ctgta
//
LOCUS       DAORHF010000026        26067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1162_length_26067_cov_6.571467, whole genome shotgun sequence.
ACCESSION   DAORHF010000026 DAORHF010000000
VERSION     DAORHF010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26067
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1162_length_26067_cov_6.571467"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PKJ16_03955"
     CDS             complement(<1..198)
                     /locus_tag="PKJ16_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HNV46172.1"
                     /translation="MDTSAVLIAFALTAIAGLSTGVGSAIAVLANTTNKKFLSLALGF
                     SAGVMMYVSFVEIFFEAKKSLV"
     gene            complement(314..1348)
                     /locus_tag="PKJ16_03960"
     CDS             complement(314..1348)
                     /locus_tag="PKJ16_03960"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV46173.1"
                     /translation="MDTTRDIHRDITRDIAAHAQSGSAHAARPKTSITTDIVRLAMSR
                     QLNPARVRFLGMRRFRAMSDLATRLIGGRRERLDVVRDITIAGRRGAVPLRLYRIDAA
                     DPARPQPVLLFFHGGAYNIGSIDTHDYICRYLARHAGAVVISVGYRLAPEHPYPAALE
                     DCYAALQWAYERAWTFNGDRTRIAIAGDSAGGGLAAKIAFMARDRKGPSLCCQLLIYP
                     VVTPEDATASREQFATRYGLNRDFIDFSRRLFVTDSALLSEMRLLTRDDLRGLPYTMV
                     ITAECDPLRDEGEIFADRLSRAGVPAERARFRGMIHGFISMGLIYREAYAALDLAAAR
                     LRTAFSGAHG"
     gene            1528..1914
                     /locus_tag="PKJ16_03965"
     CDS             1528..1914
                     /locus_tag="PKJ16_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46174.1"
                     /translation="MTPETLDAMLREGFVKREDDRNRIIAHFEELVQAQSLAEAVGIV
                     RSVCAGPLRNRDARVALGAIVLLERLYKRLPEDRFLPDKGAVRDEALAALREKMADAN
                     KKISRTASETFDLLKTYFPPIRFDLW"
     gene            complement(1924..3330)
                     /locus_tag="PKJ16_03970"
     CDS             complement(1924..3330)
                     /locus_tag="PKJ16_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46175.1"
                     /translation="MIHDDAIYNSLIQSLPDIVYKIDPEGRFTFVSNSICKLGYDPAE
                     LIGKHYSAIIHPDSRDAIQREKVLPQYQGKMLPDSKAPKLFDERRTGTRITRSLRIRL
                     LPRDPDTAPAQFDGEVISLGLYEGGNEETGQLLGTIGIIRDLSDVITIEKALRLTERF
                     YRSLIDYSSDAFSIVSTDGTILYVSESVRSFLGYTPIDLIGESLLTYIHPDDEEVFPR
                     ILSHEKTKTEGSPFFEYRFLHADWSWRFLESSARTILDERNELVSTVLYTRDVTRRKE
                     VELELQRERERLMRSLEEKNVLLREIHHRVKNNMQIITSLLQLQARATDDRRLAEQFT
                     VCIQRIKSMSLIHERLYQSDNLSSIHFTTYLRSLIADLQRAYASDRPAPTLTSDLDEV
                     VLDIEQAIPCGLIVHEILANAFAHAHPAPNGAAEAIDVLLRAGDDTISLEIGDNGVGM
                     PAGDAVPHRLSPPFAGWG"
     gene            3526..3888
                     /locus_tag="PKJ16_03975"
     CDS             3526..3888
                     /locus_tag="PKJ16_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HNV46176.1"
                     /translation="MAQKTKYSTDPDTLLAECEVDYYSGTGKGGQNRNRHYNCVRLRH
                     RPTGIIAVGTEERSQRKNRELAIRRLVERIEALNYVKPKRVPTKPSAGAKKARIESKT
                     RVAVKKSARKKVANDDDA"
     gene            3872..4648
                     /locus_tag="PKJ16_03980"
     CDS             3872..4648
                     /locus_tag="PKJ16_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HNV46177.1"
                     /translation="MMTTPSGRTRACAIIIAFCAAALVAALAAAALVHYGVIWLHDPD
                     PVRYPVRGIDVSHHQGAIEWSAVGARRIDFAFIKATEGGDHVDSRFAENWRGAASAGI
                     ARGAYHFFTLCRAGSEQARNFISIVPRENGALPPVVDLEYEGNCRNRPAEFVVLDEIK
                     AFLDAVERRYGVRPILYVTREFYDRHLRGALPGYRIWLRAVYERPVLPDGRAWDFWQY
                     SSRGRVAGIDGRVDLNVFRGTRREFDALVSASGRAPGSAP"
     gene            4672..5616
                     /locus_tag="PKJ16_03985"
     CDS             4672..5616
                     /locus_tag="PKJ16_03985"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HNV46178.1"
                     /translation="MNRLGREGVPFLFIVDFDGARPIVLPLDEVDPAAILYDVNGRRN
                     FDPPGAPIAPPRLRTTPIGYERYRRAFDLVQENLRAGNSYLLNLTFPTAIEVDAALGD
                     IVLHARAPYRLWMRDELVVFSPECFVTIERGVIASHPMKGTIDAALPDAEARILADEK
                     EFAEHVTIVDLIRNDLGIVSRGVAVERFRYVTRVRSAQGELLQVSSRITGRLDGDYPA
                     RIGDIVSALLPAGSVTGAPKKKTVEIIKAAEHYNRGYYTGVFGVFDGASLDSAVMIRF
                     IERDGGSLVYKSGGGITIYSDPEAEYRELIDKVYVPIV"
     gene            5621..6196
                     /locus_tag="PKJ16_03990"
     CDS             5621..6196
                     /locus_tag="PKJ16_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HNV46179.1"
                     /translation="MRLDDGRFDDLRYHTERFNRSRRELFGCADEIDLAARIAVPDDC
                     RAGLYKCRVVYERDIIGTEFVPYRMRTIHRLQLVREDTVDYTYKYDDKRALEALVARR
                     GVCDDVIIVKRGCVTDASFCNLAFYDGARWITPDTPLLAGTRRARLLEAGRIVEGRIR
                     PGDLGAYSTVAMINAMIDLGQLTIPIDGIVE"
     gene            6347..8098
                     /locus_tag="PKJ16_03995"
     CDS             6347..8098
                     /locus_tag="PKJ16_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNV46180.1"
                     /translation="MHGWTGTIVRIDLTEKTFSVEHPSSDIYERFIGGRGMAGHYLMR
                     DGTRAWDDPAMPLLLFTGPLVDTPTPTSGRMCVMSRSPLTGAICDTSVGGRLGTQLKR
                     AGFDGVVIAGKSDAPCGIEIRDGAITFTDAAHLRGKTVSETRAAIGDAGSCAVIGSAA
                     ENGVLYASIMVDGHFCSGRGGLGLVMASKNLKYLAVSGTGKTPVADRDEIAAAREEIN
                     RLAAASPILLGELGIAHYGTGALYDLMDSRRMMPTDNFRATHFAAAASMNAHAYKTRY
                     ATKRTGCTGCAILCKKIGANKEIIPEFETMSHFSALIGNVDPDAVIEANRVCNEAGMD
                     TITAAVTIACHLEITGRSLSPDALIELLNDIAHARGDGAALAMGSHRYAAAQGRPEVS
                     ISVKKMELPAYDPRGAYGMALAYAVSTRGACHLRAYPISHELLRKPVATDRFSWSGKA
                     RIIKIAEDANATIDSLTACKFLFFAASLEEYARAYCGATGARATAQDLLAAGERIYYT
                     ERIMNAANGFSADDDDLPPRFFAEPGTGGGGIAIAPLGRGEFLAARARYYRIRGLDGR
                     GMPTEEKCRELGIEWNG"
     gene            8086..9261
                     /locus_tag="PKJ16_04000"
     CDS             8086..9261
                     /locus_tag="PKJ16_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HNV46181.1"
                     /translation="MERLIRKYEDKLVAHGLCDPGAPLIGSRDEAVLWNRDDALVPAM
                     EEVFAGLNIASLLFARPAEPFFSIMERVAHSPEARAGCIRPQDSETRTFLHDIPVLDA
                     FVPRDIIATLKRRKCVVVRSHGIVTYGTVSPEQAFVFFSSVCFSIYVKFFADYRDAVR
                     AGTADAADRAVVERALASYESFVARIPESPRLMRGPFTEPDDCLAAIVEAGRMTVECR
                     MVDSFFGNISYRLGDTVYISQTGSSLDELGGMIDPCPMDNSSCAAITASSELSAHRGI
                     YRDPGVAAILHGHPKFAVIASLDCDDDACAHRGRCHVECARERFVGDVPIVPGEVGTG
                     PHGLCNTLPPAMRGRRGAIVFGHGLFTTGAADFTDAFASLVSVERMCLEEYRQTITG"
     gene            9694..10080
                     /locus_tag="PKJ16_04005"
     CDS             9694..10080
                     /locus_tag="PKJ16_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46182.1"
                     /translation="MIILLLPIGDTAHLVISVIVGIGFMYYYIFAALANWPGDDDPLE
                     PVKLSYPIMMLLLWTFGWSIESVIGRDLEGGVRLLEHSIRHQNLSAIRWRPIACMVFV
                     PAMWIAGVTLWELGLLYRTKARGNRL"
     gene            complement(10150..10971)
                     /locus_tag="PKJ16_04010"
     CDS             complement(10150..10971)
                     /locus_tag="PKJ16_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV46183.1"
                     /translation="MRILLSGIIGAIFLLSCKPTPGVCPVRICGSTTLYPIMTAALAS
                     LSRANREAVELSATGSVGGYRSLLDGACDICDSSYRLAPQEIDALAKKGIRVREIAIG
                     YDLVIPIVHPSNPLGEIAPDALRGIFAGTVASWKELRGPDLPIKPVCRDEGSGTGAVW
                     SKRFMRDGPCASGSLRARSNSESLSTVAHEPGAIGYISRAFLNPEVKELKIAGHPTDP
                     AAMKRHPGFRTLYLYVNEKNECPAGRALLVYLMSAGGKELLRNHRFTPATDGGMR"
     gene            complement(11086..11967)
                     /locus_tag="PKJ16_04015"
     CDS             complement(11086..11967)
                     /locus_tag="PKJ16_04015"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HNV46184.1"
                     /translation="MKLPFNANARINRRIALLIAILFMDEAVYLVNLLGFYPVPPEII
                     TAARTLSIVIIGFLVVNVILRFTENRVFHFLEGEVEIEQRILLTKLYKGALYLIPVLF
                     LLVHLGLSFQNLTLFVGLVATGIALAMRELIMSYLVWIIIILKNPVRIGDGIRIGDTT
                     GLVSRIGTMYLSVRPCPDVTERVVRIPNKTLLDHPIVNFGRGVIADAIRIPLETPPRD
                     WAAPMERIRASIERNLPGVSALSLRIDIDAGKWVLAVEYTVDFAERDAARTRLLGTIA
                     ELYQDLIAPAGRPGKRR"
     gene            complement(11967..13001)
                     /locus_tag="PKJ16_04020"
     CDS             complement(11967..13001)
                     /locus_tag="PKJ16_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46185.1"
                     /translation="MRARNHTRARPSGRTAAVGCLLVMLALPGAGAAETGLGYREFRL
                     GQSIAEARGIIAASYRGMDAQHDAAANAITITQNEQNNERVILRFNAAGTLYAITTLL
                     GRDKDSIYLSKLLNFLENKYGKTTVESGGANRGFIWRAVWRLQNRRYGINAEYTATGV
                     AITYRDIAADERMQGESLRTRYDAFKALFVPRAGSAPATLRFAEFRLGDTRAAVVSTL
                     ARLGARPGADTAPGKCRNGRAIVTARLADRSEIRVSFDNADAAYEIVVYRPDADQRYI
                     VDLLRFMRSVAGEPVYANEEMYIGRWEFEGGALLFTASFTDRATVSLIDKTRLDRCIA
                     DVEKSLFKDL"
     gene            13128..14615
                     /locus_tag="PKJ16_04025"
     CDS             13128..14615
                     /locus_tag="PKJ16_04025"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV46186.1"
                     /translation="MINTSELTDRLIDGGTDITLAFKHPDDRTIIAISALVSKILSRL
                     DRVFFIDSLIIIVKEIVLNAYKANAKRWYFSSRGLDIADPEQYAAGMARFDHEVVRNL
                     ERIRDDMERSDYTITLVISKRPEGIILSITNNVPILPDELERVRARLRKAGDFDNLRD
                     VYTSLYDTTEGAGMGIILSSLLLRHYGIDASSLTVTPGENSVTTSLTVPYEFRPREVV
                     SIIRDRIVAAIEGLPTFPKNIVELQGMCADPSVAIEAIAERITHDPCLSAEILKVANS
                     AGFVTRKRIETVHEALMIIGIKNLQTILVVTAARKILAQRYHKFEELWEHCTKTAFYA
                     RRIAHALKLGAAADHAFLAGLLHDIGKIVLLSTDLTLVNQIADLVANRKVRASTIIEE
                     ATVGISHGAIGELIARKWNFPAFLIEAIRDHHTPLNAPPEYSVSVSVVYLANMLVNIE
                     SNDCAYSYVETQVLERFGIAGEDAFNALHDQIRTEYAEHSAAMNA"
     gene            complement(14632..14883)
                     /locus_tag="PKJ16_04030"
     CDS             complement(14632..14883)
                     /locus_tag="PKJ16_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46187.1"
                     /translation="MEPHQAHTVAQILKSGKVYTDGRSVGLERLSYDRANLTFIYRKE
                     DATADIFNPVVFESLMSDEEFLAFVESNFDFDAVIEKLV"
     gene            complement(14993..17317)
                     /locus_tag="PKJ16_04035"
     CDS             complement(14993..17317)
                     /locus_tag="PKJ16_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HNV46188.1"
                     /translation="MTTRTLAHILQTAPIVMMIACSGAHSSSDVRERIAVDLCARMAD
                     ARIEDADAAVKRRDRERFDNLSTTPWGPDSPYLVTAGFVMDRAKKHQRIRQSIWLSAH
                     RGAPVEMRIGLGDGPVRDGTLEFSLASIRRASPRAARRLIITFSHGGASIPLYDSARD
                     EHGIPDSRWRGHAIALPRGAGAHATLALRFESDADRPEHLFVGGPRIVTPTREAPTNV
                     VFVIVDAMRADCLNALADRYAVTPVMDVLARDGVLFERHIVAANWTRPSTIAMLGSCS
                     ASAAGVNLFYPAVHDEERAFFYRESGVTLLPTLLKRAGHLTRAIGNNPFIIDYSGIGV
                     DCDLADLSEYSTSVADTTDITEETVAWLGENARRPFFLFMNYNAPHNPYSPPARFYRE
                     AARRFPGADPMFNAYLGEIAYTDEGLGRVIAALKRLGLYENTLIIVTSDHGEIFSHDR
                     GSSPFTGADTIYTHGQTQFDEELHTPLIVKFPAAYGYAGKRVAAQVRSIDIAPTVLEA
                     LGLSPPDSFQGRSLFPLVRGEERQERIAYSEGRRMYSVRYGGYKYAERFTGFGLKPEH
                     WGGEYAGEYAELYDLSADPRETRNLAADLPETAGRMRALLRHARFKQPDNVLFSNGAP
                     ARGRVRIENGFFYSIATTGAGSSSRALSRRECEFSLPAGGSLVFQTIPGAARVAITAA
                     PATPLLCGRNLLPLAARSLGGTLAINGEDRALHGRPEPAMLALAGRALLFWHEGAKSS
                     VREVKREKYLSADINVILKDWGYIQGKERTREED"
     gene            17505..18149
                     /locus_tag="PKJ16_04040"
     CDS             17505..18149
                     /locus_tag="PKJ16_04040"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV46189.1"
                     /translation="MKRTCTWLMALAAATAIGCSTIDVAVFPTAEFRQQVKKIAVMPF
                     NFKGQDIGQDFAGAIRTYLMQEAPGRFIIVEREEFDRIVDEQKMGATGLFDEGQAAKL
                     GKMIGADIMIVGRGDVLELTGPGTYPGKHIHTCEIKVVSVEKGSLLVSLIKTPGIAWD
                     MGYRAKWCFSPFGAGIWKDADLVAQSSMYSNLARQCVKRILGAIERVEKRPLGY"
     gene            18229..19020
                     /locus_tag="PKJ16_04045"
     CDS             18229..19020
                     /locus_tag="PKJ16_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46190.1"
                     /translation="MIMGTIRTAVARIAAALVVAAVAITPCAAIEFDELDKPPEGAHK
                     GQMFLGVVISVGAPSGELVDAETAFVDGSTYTFENEVTKQLKVSHLFFGVGASFEYMP
                     IDHVGAKTRAMQTIVVQRTEFGTDYENWRENLYTCGAIYAGPAFHLTTRRSWDVTLAA
                     LAGYGWGEYAATPVAKKLIENYTGENKKTASSFTYLVELAFCAYFSGGLYFSIGGEFI
                     SYAVDFGKPFELVNPQTQKKYYEGKSAGAITSVNCVLSIGYAFSN"
     gene            complement(19129..20988)
                     /locus_tag="PKJ16_04050"
     CDS             complement(19129..20988)
                     /locus_tag="PKJ16_04050"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46191.1"
                     /translation="MNVFILLHVTASLFYLYLAGFILSRGLRDGLNRVFSLLCALFGL
                     WAASFAVMHAFHAPEMAYAAYTISALAWCLYSGMAAHVLLYIAGSGLTRNPWFVTALY
                     APGILLLAMQWTGHFQGFALYRHPLGWIEIPLTDSPWYWFFILYQVLCISVALIILVC
                     WGLRSPLRRERKQSAILTICISFTFLLCFISDILLPLIGVYAMPSLSPLIIVLLAAGT
                     LFTIVRYQLLSLSLAFASGEILKRIRDVVVLLDPGGTVLKVNAWAGTLLGSSETELAG
                     RPFTEFLRNRDAFRTLFDAVRAGDREEFSLEDALITRSGGEVPHRISGSAMHDREGDI
                     IAVLIIAHDLTEKILLRREIEQGIATAAELRQSREVLSARNEQIEEQLLHAQRIQRAL
                     LPQSAPITERVRTDFRSNSMDAVGGDYFSFAATSEGLGLLIGDVSGHGVSAALFLALL
                     KSESERIFRTSHRDPAGFIGTLNLNLLGAMPGHYLTAAYAFFSWTGQGPVSLAFSKGG
                     HPEPVLQRSGRPAEFLVCHGPVIGHFAHATFCDMRLELGTGDRVFFYTDGIVETRTAD
                     GELFGYVRLLSLIDEFAHLELSDALDRVEKAIASFRGAAPAEDDSVVIGCEIL"
     gene            complement(20985..22031)
                     /locus_tag="PKJ16_04055"
     CDS             complement(20985..22031)
                     /locus_tag="PKJ16_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HNV46192.1"
                     /translation="MTASRYLAADPIPLLLEARHPHLRYRTLRDLVGLTDDDAELREA
                     RERLSGTAHAARLRRAIHGGVVGDARRFDLYGSGMMWRFIEAVALGFDRRDDFVRDTA
                     ERILARCQLPDGGFTLAWRPAIALACRTGDMLRALIEAGYADDARVLRGLSWIRERQR
                     SDGGWLHCPQEGWRDALGLVLLGRSGGGLRREGRTAVPSCPIATHACTDALLRHARTT
                     GRAPEGALDGAAEFFLAHRCFVRPNRSDIPSRCERGGLHEWSSLGYPLMTHYDILAGL
                     SVVAGAGRIDDSRSGEAFNTLIEKQNADGTWAMERFSSGMLFGSRRTIDDEEARKWTT
                     LRVLQFFNDAGLAT"
     gene            complement(22028..23740)
                     /locus_tag="PKJ16_04060"
     CDS             complement(22028..23740)
                     /locus_tag="PKJ16_04060"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV46193.1"
                     /translation="MIARRVAIVCSAALLAVSSAPEAGAQSYRWPIDVPKRVTSTFGE
                     FRSTRFHHGLDVSTGGKRGFRVVAAERGYVSAVMYENYGIGYAVFVRHPDGRTTFYGH
                     MDRFADGILANARVRRHIEHIRNHRDFRVDFSKPEIPLRRGTPIGYSGDSGAGLEHFH
                     FEVRDKNNVHLNPLRYALWVPDATAPVIETVYLTPLDRFSRVDGTPGEKAYRATLVNR
                     KEGRYVLAGNVVPRIAGRVGVKARVHDTVGWRNKVTAYGFNWYVDGVKRHRLFFDKIV
                     RAYTHRMGLIYDYDTSNSRHFTFYLYARTSGDGVINAGAPGGTRRARAEFFDARLNRS
                     IIEFSCRAEPPAPEPAPGFDHNLVPGSALVLTSADGRCTLSFPDTAALYPEMVAMEQS
                     PLPQNVLSGLPVLSARYAVTPTHLCIDSTARIRIAYDGADHRTVGIYSVNAAGTGIGF
                     VSNSYHPKERAFYATVRRMGRYVLARDEIPPVIRHRNPKRPRPNTQLRFTVSDRGSGV
                     DLSKISLAVDGKSVFWYFDVDYRAVVILPHNPIWAKGAHTVTMTIVDRAGNRSAPLTY
                     EYEQ"
     gene            complement(23784..24512)
                     /locus_tag="PKJ16_04065"
     CDS             complement(23784..24512)
                     /locus_tag="PKJ16_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46194.1"
                     /translation="MKTILLLCAILCVALPASADSYDDALKLFQQNKYDDALKTITDT
                     LDAKRDREPGSPNYRLRFLAAHAHWKKGNLKGALSHFRKCAEIDRATPDPYIDAALML
                     VEHRKLKEADSFLRKGRKIKDDPMFYYIWGRMALANRNAWRAKELLEKSISMDPALYA
                     SYHALGMALMRLKRYSEANTAFATAHELMPSSGIILNNLALSYELLGKNAEAVEAYRK
                     AQKLAPENTAIFDNLSRAEKLTKK"
     gene            complement(24576..>26067)
                     /locus_tag="PKJ16_04070"
     CDS             complement(24576..>26067)
                     /locus_tag="PKJ16_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HNV46195.1"
                     /translation="VHGMHVAEAILTAKGGMTSHAALVARGWGKCCIVGAGAIHVDVH
                     AKTMTVDGRTFREGETLTLNGTRGFVYAGALAMMDATENPRFQKFMKIADEMRTLKVR
                     TNADTPEDAAKAREFGAEGIGLFRTEHMFYGKNSEQPLFILRKMILSDTEEKRRAALD
                     ELFPFVKRDIKSTLAVMSPLPVTIRLLDPPLHEFVPTRQDEREKLASALGIGMDRLTK
                     RAEDLHESNPMMGHRGVRLGVTFPEISEMQYRAILESAAELNKEGKKVVPEIMIPVVG
                     HVNELDDQKKLLDAVHAAVCKKMGVSKIEHMFGTMIEIPRAAITADRIAKTAEFFSFG
                     TNDLTQMTFGFSRDDIGGFVPDYVEKKILPVDPFQQLDRDGVGVLMRMAVEKGRSVRK
                     DIKLGICGEHGGEPSSVEFCHMIGLSYVSCSPFRVPIARLAAAQAAVRGAALPKAKPA
                     VKKAAPKKKAAPKKPAAKKKAAPAKKKPAPKKKAAPKKTAKKKGKK"
BASE COUNT         4577 a         8079 c         8811 g         4600 t
ORIGIN      
        1 gacgagggac tttttcgcct cgaagaatat ctcgacgaag gacacgtaca tcatcacgcc
       61 cgccgaaaag ccgagggcca gcgacaggaa ttttttattg gtggtattgg ccaggacggc
      121 gatggcgctc ccgacgccgg tggagaggcc cgcgatggcg gtgagcgcga aggcgatgag
      181 gaccgcggaa gtatccattg gcgcgactcc gaagcagtgc tgtatcatgc tatcaaaaac
      241 ggacgggccg ttcgagggca agcactaatt ctatttcgag gatgaccccg gtcgcgccgc
      301 gtggaatgcg ccttcagcca tgcgcgccgg agaaggccgt ccgcagccgg gcggcggcga
      361 ggtcgagggc cgcgtaggcc tcgcggtaga tgaggcccat ggagatgaag ccgtggatca
      421 tgccgcggaa gcgcgcgcgc tcggcgggga ccccggcgcg ggagagccgg tccgcgaaga
      481 tctcgccctc gtcgcgcagc ggatcgcact ccgcggtgat gaccatggtg tacgggagcc
      541 cccggaggtc gtcgcgcgtc agcaggcgca tctcggagag gagcgcgctg tcggtcacga
      601 agagccttct cgaaaagtcg atgaagtcgc ggttgagtcc gtagcgcgtg gcgaattgct
      661 cccgcgaggc cgtggcgtcc tcgggggtga ccaccgggta gatcaggagc tggcagcaca
      721 gggacggccc cttccggtcg cgcgccatga aggcgatctt ggccgcgagg ccgccgcccg
      781 cgctgtcgcc ggcgatggcg atccgggtgc ggtcgccgtt gaaggtccag gcgcgctcgt
      841 acgcccactg cagggcggcg taacagtcct cgagcgcggc cggatagggg tgctcgggcg
      901 cgaggcggta tcccaccgag atcaccacgg cgccggcatg ccgcgccaga tagcggcaga
      961 tgtagtcatg ggtgtcgatg ctgccgatat tgtaggcgcc gccgtggaag aacagcagca
     1021 ccggctgggg gcgcgcgggg tcggcggcgt caatgcgata gaggcgtagg ggaacggccc
     1081 cgcgccgccc ggcgatcgtg atatcgcgca cgacgtcgag gcgctcgcgg cgcccgccga
     1141 tgaggcgcgt cgcaaggtcg gacatcgcgc ggaagcggcg catgcccaga aacctgaccc
     1201 gggcgggatt gagctggcgg ctcatggcga ggcgtacgat gtcggtggtg atgctggttt
     1261 tcgggcgtgc tgcgtgtgcg gatccgctct gcgcatgggc ggcgatgtct cgtgtgatgt
     1321 cccggtgtat gtcgcgggtg gtgtccatgg gtggcctctg tgacgggtgc gtgatcgtgc
     1381 gtggtgtaca aacgaatgcg atttgtgcaa cgatttcggc ggcggcgcgt tttggcaacc
     1441 gaatttttct ttcgggaccg gtgggccatc gacccatggc gggtaatagt gattgacgcg
     1501 cgccgctgtg cgtgatacaa gcggcgtatg acacccgaga cgcttgatgc gatgctgcgc
     1561 gagggattcg tgaagcgcga ggacgaccgg aaccggatca tcgcgcattt cgaggagttg
     1621 gtgcaggcgc aatccctggc cgaggccgtc gggatcgttc ggtccgtttg tgctgggccg
     1681 ctccggaaca gggatgcgag ggtcgccctg ggggccatcg tgcttctcga gcggctgtac
     1741 aagcggcttc ccgaggaccg gttcctcccg gacaagggcg ccgtgcgcga cgaggcgctc
     1801 gcggcgctca gggaaaaaat ggccgatgcc aacaaaaaga tatcccgaac agcctctgaa
     1861 acgttcgacc ttctgaagac ctatttcccg ccgatccgat tcgacctctg gtagcgttag
     1921 gcgctatccc catccggcga acggcgggga aaggcgatgc ggtaccgcgt cccccgccgg
     1981 catgcccacg ccattgtcgc cgatctcaag cgatatcgta tcgtccccgg cccggagcag
     2041 gacgtcgatg gcctccgccg cgccgttcgg tgcgggatgg gcgtgcgcga aggcattggc
     2101 gagtatctcg tgcacgatga gcccgcaggg aatcgcctgt tcgatgtcca gcacgacctc
     2161 gtccaggtcg ctcgtcaacg tcggcgcggg ccggtccgag gcatacgccc gctgcagatc
     2221 ggcgatcagc gagcgcaggt aggtggtgaa gtgtatgctg gacaggttgt ccgattggta
     2281 gagccgctcg tggatcagcg acatgctttt gatccgctgg atgcacacgg tgaactgctc
     2341 ggccagcctg cggtcgtcgg tcgcgcgcgc ctgcagctgc agcaggctgg tgatgatctg
     2401 catgttgttt ttcacccggt ggtggatctc gcgcagcagg acgttttttt cctcgagcga
     2461 ccgcatgaga cgctcgcgct cgcgctgaag ctcaagctcg acctccttgc gccgggtgac
     2521 gtcgcgggtg tacaggaccg tgctcaccag ctcgttgcgc tcgtcgagga tcgtccgggc
     2581 cgatgattcg agaaaacgcc acgaccagtc ggcgtgcaga aaccggtatt cgaaaaaggg
     2641 gctgccctcg gtcttcgtct tttcgtggga gaggatgcgg ggaaagacct cctcgtcgtc
     2701 gggatggatg taggtgagca ggctttcgcc gatcaggtcg atgggggtgt agccgagaaa
     2761 gctcctcacg gactcgctca cgtacaggat ggtcccgtcg gtggagacga tcgagaaggc
     2821 gtcagacgag tagtcgatga gcgagcggta aaagcgctcg gtgaggcgca gcgccttttc
     2881 gatcgtaatc acatcgctca ggtcgcggat gatgccgatg gtccccagga gctggccggt
     2941 ctcctcgttg ccgccctcgt agagccccag cgaaatgacc tccccgtcga attgcgccgg
     3001 tgcggtatcc ggatcgcgcg gcagaagccg tatcctgagc gagcgggtga tgcgcgttcc
     3061 cgtgcgccgc tcgtcgaaga gcttgggcgc cttgctgtcg gggagcattt tcccttggta
     3121 ctggggcagg accttctcgc gctggatcgc atcgcgcgaa tccggatgga tgatggcgct
     3181 gtagtgtttg ccgatgagct cggcgggatc gtagccgagc ttgcagatgg aattgctgac
     3241 gaaggtgaac cggccctcgg ggtcgatctt gtagacgatg tcgggcagcg actgtatgag
     3301 cgagttgtag atggcgtcgt cgtgtatcat gtccgtttcg cgctctcccc cgtgcgctcg
     3361 tatcattgac gcgtttttac ggcatgtcaa cgagaaaacg cccgacgctc gatgaaaagc
     3421 gtgcgaggtg aagcccgcct gtgcggtatt aatgcttgat aaaatcgcgc accgtcatta
     3481 cgtggtgcgc gcgcaggcgc cgcgaatcgc gctgcgtcaa cggccatggc acagaaaacg
     3541 aaatacagca ccgacccgga caccctcctc gcggagtgcg aggtcgacta ttacagcggg
     3601 accgggaagg gcgggcagaa ccgcaaccgg cactacaatt gcgtgcggct tcgccaccgc
     3661 cccacgggga tcatcgcggt ggggaccgag gagcgctcgc agcggaaaaa ccgcgagctc
     3721 gcgatccggc ggctcgtgga gcggatcgag gcgctcaatt acgtgaaacc gaagcgcgtc
     3781 cccacgaagc cgtcggcggg cgcgaagaag gcgcggatcg aatcgaagac gcgtgtcgcg
     3841 gtgaaaaaat ccgcgcgcaa gaaggttgcg aatgatgacg acgcctagcg ggcgaacgcg
     3901 cgcatgtgcg atcatcatcg cgttctgtgc ggcagctctc gtcgcggcgc tggccgcggc
     3961 ggcgctcgtg cactacggcg tcatctggct gcatgatccc gatcccgtcc gctatccggt
     4021 gcggggcatc gacgtgtccc atcatcaggg cgcgatcgag tggagcgcgg tgggcgcgcg
     4081 gcggatcgac ttcgcgttta tcaaggcgac cgagggcggc gatcacgtcg attcgcgctt
     4141 cgcggaaaac tggcgcggcg cggcctcggc cgggatcgcg cggggagcct atcacttctt
     4201 tacgctgtgc cgggcgggga gcgagcaggc gcgcaacttc attagcatcg tgccgcgcga
     4261 gaatggcgcg ctgccgccgg tcgtcgacct ggagtacgag ggcaactgcc ggaaccggcc
     4321 cgcggaattc gtcgtgctcg acgagatcaa ggcgttcctc gacgcagtgg agcgccgcta
     4381 cggcgtgcgg cccatcctgt acgtgacccg cgagttctac gaccggcacc tgcgcggcgc
     4441 cctgccgggc taccggatat ggctgcgcgc cgtgtacgag cggcccgtac tcccggacgg
     4501 tcgggcctgg gacttctggc agtacagctc gcgcggccgt gtcgcgggga tcgacggccg
     4561 ggtggacctc aacgtgttcc ggggaacgcg gcgcgagttc gacgcgctgg tgtcggcgag
     4621 cggacgcgcc ccgggaagcg cgccgtgatc gagccgtccg cgcgcgagcg catgaaccgc
     4681 ctgggtcgcg agggcgtccc ctttctcttt attgtcgatt tcgacggtgc gcgccccatc
     4741 gtccttccgc tcgacgaggt ggaccccgcc gcgattttgt atgatgtaaa cggacggcgt
     4801 aatttcgatc cgcccggcgc gccgatcgcg ccgccacgat tgcgcacaac gccaatcggg
     4861 tatgaacgct accgccgcgc cttcgacctg gtgcaggaga acctgcgcgc ggggaacagc
     4921 tatctgctca atctcacctt tccgacggca atcgaggtcg atgcggccct cggcgacatc
     4981 gttctgcatg cccgcgcacc gtaccggctc tggatgcgcg acgagctcgt ggtgttctcg
     5041 ccggaatgct tcgtgaccat cgagcgcggg gtgatcgcct cacacccgat gaagggcacc
     5101 atcgacgcgg ccctgccgga cgccgaggcg cgcatcctcg ccgacgagaa ggaattcgcc
     5161 gagcacgtca ccatcgtgga cctcatccgt aatgacctgg gcatcgtctc gcgcggcgtg
     5221 gcggtggagc gctttcggta tgtcacgcgc gtgcgatccg cccaggggga gctcttgcag
     5281 gtgagctcgc gcatcacggg ccgcctcgat ggcgactatc ccgcgcgcat cggggacatc
     5341 gtgagcgcgc tcctcccggc cggctcggtg acgggcgccc ccaagaaaaa aactgttgaa
     5401 ataatcaaag ccgccgagca ctacaaccgg ggttactaca ccggcgtctt cggcgtcttc
     5461 gacggcgcct cgctcgacag cgcggtgatg atccgcttca tcgagcgcga cggcggctcg
     5521 ctcgtctata agagcggcgg cggcatcacc atctacagcg acccggaggc ggagtatcgc
     5581 gaactcatcg acaaggtcta cgtgccgatt gtttgaggcg atgcgcctcg atgacggccg
     5641 gttcgacgat ctgcgatatc acacggagcg gttcaaccgc tcgcgccgcg agctcttcgg
     5701 ctgtgcggac gaaatcgacc tcgccgcgcg gatcgcggtc ccggacgact gccgcgccgg
     5761 tctgtacaag tgccgggtgg tgtacgagcg ggacatcatc ggcacggaat ttgtcccgta
     5821 tcgcatgcgg accatacatc gattgcaact tgttcgcgag gatacggtcg actatacgta
     5881 caagtacgac gacaaacgcg cgcttgaggc gcttgttgcc cggcgcggcg tctgcgacga
     5941 cgtcatcatc gtgaagcgcg gatgcgtcac ggacgcctcg ttctgtaatc tcgcgtttta
     6001 cgacggcgcg cgctggatca cgccggacac gccgctcctc gcgggaacgc ggcgcgcgcg
     6061 cctgctcgag gcgggaagga tcgtcgaagg gcgtatccgc ccaggagacc tgggagcgta
     6121 ttcgaccgtc gcgatgatta acgccatgat tgatctcgga caactgacaa tccccatcga
     6181 tggtattgta gagtaaatga tgaacggcac cgcaccccgc aaccggcgag acctgcgcct
     6241 cgtcccccgt tttgtgattc cccggggagc gggccgggcg cacattgtat ccttgcctga
     6301 ccccccttct ccggggggag aaggggtcgg gggatgaggt agcatcatgc acggctggac
     6361 cggaaccata gtgcgaattg acctgacgga gaagacgttt tccgtcgaac atccgtcatc
     6421 cgacatctac gagcgattta tcggcggtcg tggtatggcg ggccactacc tcatgcgcga
     6481 cggcacgcgc gcctgggacg atccggcgat gccgctcctc ttgttcaccg gaccgctggt
     6541 ggacacgccc acgcccacct cggggcgcat gtgcgtcatg agccgctcgc cgctcacggg
     6601 cgccatctgc gacacctccg tgggcggaag gctcggcacg cagctcaagc gcgcggggtt
     6661 cgacggcgtc gtgatcgcgg gaaaaagcga tgcgccctgc ggcatcgaga tccgcgacgg
     6721 cgcgatcacc tttacggacg ccgcgcacct tcgcgggaaa acggtaagcg agacgcgtgc
     6781 ggccatcggc gatgccggtt cctgcgcggt cattgggtcg gcggcggaga acggcgtcct
     6841 ctacgcgagc atcatggtgg acgggcattt ctgctctggg cgcggcggcc tgggcctggt
     6901 gatggcgtcg aaaaacctga aatacctcgc ggtgagcgga acgggaaaga cgccggtcgc
     6961 cgaccgcgac gagatcgccg cggcgcgcga ggagatcaac cgcctcgccg cggcctcgcc
     7021 catcctgctg ggcgagctcg ggatcgcgca ctacggcacg ggcgccctct acgatctcat
     7081 ggacagccgg cgcatgatgc ccaccgacaa cttccgcgcg acccatttcg ccgccgccgc
     7141 ttccatgaac gcgcatgcct acaaaacgcg ctatgccacg aagcgaacgg gctgcaccgg
     7201 ctgcgccatt ctctgcaaga agatcggcgc caacaaggaa atcatccccg agttcgagac
     7261 gatgtcgcac ttcagcgcgc ttatcgggaa tgtcgatccc gacgccgtga tcgaggcgaa
     7321 ccgcgtctgc aacgaggcgg gaatggacac catcaccgcc gcggtcacga tcgcctgcca
     7381 cctcgagatc accggccgct cgctctcgcc ggacgcgctc attgaattgt tgaacgacat
     7441 cgcgcatgcg cggggtgacg gtgcggcgct cgcgatggga tcgcaccgct atgcggccgc
     7501 gcaggggcgt ccggaggttt ccatcagcgt gaaaaaaatg gagcttcccg cctacgatcc
     7561 gcgcggggcc tacgggatgg cgctcgccta cgccgtgtcc acgcgcgggg cctgccacct
     7621 gcgcgcgtac ccgatatcgc acgaactcct gcgcaagccc gtcgcgaccg accgtttcag
     7681 ctggagcggc aaggcgcgca tcatcaagat cgccgaggac gcgaacgcga cgatcgattc
     7741 gctcacggcc tgcaagttcc tcttcttcgc cgcgagcctc gaggaatacg cgcgcgccta
     7801 ttgcggcgcg accggcgcgc gcgcgacggc gcaggacctg ctcgcggccg gcgagcgcat
     7861 ctattatacg gagcgcatta tgaatgcggc gaacggcttt tccgcggacg acgacgatct
     7921 gccgccgcgc ttttttgcgg agccggggac cggcgggggc ggcatcgcca tcgccccgct
     7981 gggtcgcggg gagttcctcg cggcgcgcgc gcggtattac cggatccgcg ggctggacgg
     8041 gcgagggatg cccaccgaag aaaaatgccg ggagctcggg atcgaatgga acggctgata
     8101 cgcaaatacg aggacaagct cgtcgcgcac ggcctgtgcg atcctggggc gccactcatc
     8161 ggatcgcgcg acgaggccgt gctctggaac cgcgacgatg cgctcgtccc ggccatggag
     8221 gaggtgttcg cgggcctgaa catcgcgagc ctgctcttcg cgcgaccggc cgagccgttc
     8281 ttttcgatca tggagcgcgt cgcgcattcg ccggaggcgc gcgcgggctg catccgcccg
     8341 caggactccg agacgcgcac ctttctgcac gacatccccg tgctggacgc gttcgtcccg
     8401 cgcgacatca tcgccacgct caagcggcgc aagtgcgtgg tggtgcgcag tcacggcatc
     8461 gtgacctacg gcacggtgag ccccgagcag gccttcgtgt tcttcagctc ggtgtgcttt
     8521 tccatatacg tgaaattttt tgcggattat cgcgacgccg tgcgggcggg aacggcggat
     8581 gccgcggacc gggcggtcgt cgagcgcgca cttgcgtcct acgagtcgtt cgtcgcgcgc
     8641 atccccgaat cgccgcgact catgcgcggt cccttcacgg aaccggacga ctgcctcgcg
     8701 gccatcgtcg aggcgggcag gatgacggtc gagtgtcgga tggtcgattc gttcttcggc
     8761 aacatatcgt atcggctcgg cgacacggtc tacatcagcc agacgggaag ctcgctggac
     8821 gagctcggcg ggatgatcga cccgtgcccg atggacaact cctcgtgcgc ggccatcacc
     8881 gcctccagcg agctctcggc gcaccgcgga atctaccgcg atcccggcgt cgccgcaatc
     8941 ctgcacgggc atccgaagtt tgcggtgatc gcgtcgctcg attgcgacga cgatgcgtgc
     9001 gcgcaccgcg gccgctgtca tgtggagtgc gcgcgggagc gcttcgtggg agacgtgccc
     9061 atcgtgcccg gggaggtggg gaccgggccg cacggcctgt gcaataccct gcctccggca
     9121 atgaggggtc ggcgcggcgc gatcgtcttc gggcacggtc tcttcacgac cggcgcggcg
     9181 gacttcaccg acgccttcgc gagcctcgtt tccgtcgaac ggatgtgcct ggaggagtac
     9241 cggcagacca tcaccgggta gagtgatcgt tttcttggtt gtacccatct cgatagcgcc
     9301 gtccggcgct gcattgaaaa tgtgttgtca tatgcgccca tttcgtctat tgaaggaaaa
     9361 gcacgggaac gaaggcgaaa ccgtacttgt agcggaggca tcgcggattg aggcgcggaa
     9421 tacgtccctc gacctccagc gagccggatg acgcgatcac aatttgcgga aaccactggt
     9481 gcaatggcaa tgaaggcccc gcctgcgaaa aaaacgaaac ccgttaagcg ttttatcaat
     9541 cccgagagcg ggcgatgcgt gttgtgcggg agggaataca aaaatctcgc gcggcacatg
     9601 gccacacgac acaaggcgat gccgccattt cgcctacggc ttggttttta cggtcggtgg
     9661 ctgtgcagta ttttcatcat gctggcgaca tcgatgataa ttctccttct gccgatcggc
     9721 gacaccgccc atctcgtgat cagcgtcatc gtcggtatcg gattcatgta ctattacatc
     9781 ttcgcggcgc tcgccaattg gccgggtgac gacgatccgc tcgagccggt aaaactatca
     9841 tatcccataa tgatgttgtt gctatggacg tttgggtggt cgattgaatc ggtgatcggc
     9901 agggacctcg agggaggggt tcgtctgctg gaacactcta tccggcacca gaacctgagc
     9961 gctatccgat ggagaccgat cgcgtgcatg gtcttcgtcc cggcgatgtg gatcgcgggc
    10021 gtaacgctgt gggagctagg tcttctgtac cgtacaaaag ctcgtggtaa tcgcctatag
    10081 cgcctgcgta cggtgacgaa acgagtgcgg ggacgctcgc tgtttgatga tgacgaagcg
    10141 cgcgaaaggc tatcgcatcc cgccgtctgt ggccggtgtg aatctgtggt tacgcagaag
    10201 ctcctttccg ccggcgctca tgagatagac cagcagggcg cggccggccg ggcactcgtt
    10261 cttttcgttc acgtacagat agagcgtcct gaatccggga tgccgcttca tggcggcggg
    10321 gtccgtggga tgacccgcaa tcttcagttc cttcacctcg ggattgagaa aggcgcggct
    10381 gatatagccg atcgcccccg gctcatgcgc caccgtcgag agcgactcgc tgttcgaccg
    10441 cgcgcggagg ctgccgctcg cgcaggggcc gtcgcgcatg aaccgcttgc tccagacggc
    10501 gccggtgccg gaaccctcgt cgcggcatac gggcttgatg ggcagatcgg ggccgcgcag
    10561 ctctttccac gatgcgaccg ttcccgcgaa gatgccccga agcgcgtccg gggcgatctc
    10621 gccgagcggg ttcgagggat ggacgatcgg gataacgaga tcgtatccga tcgcgatctc
    10681 cctgacgcga atcccctttt tcgcgagcgc gtcgatctcc tgcggggcga gccggtagga
    10741 cgagtcgcag atgtcgcagg cgccgtcgag gagcgaccgg tatcccccga ccgatcccgt
    10801 cgcgctgagc tcgacggcct cccggttcgc gcgggagagc gaagcgagcg cggcggtcat
    10861 gatcgggtac agtgtcgtcg atccgcatat ccgcaccgga cagacgccgg gcgtgggttt
    10921 gcaggacagc aggaaaatcg ctccgatgat tccgcttaaa agaatgcgca tgggacgctc
    10981 caggatgtgg tatcgagcgg cgcgcggacg ctctgtatca cggacggcgg ttttcgcgaa
    11041 aagtcaaacg attatcgggc caatcgcgta aaaaccggcc gcgcgctatc gccgttttcc
    11101 cggtcgtccg gcgggcgcga tgaggtcctg atacagctcg gcgatcgtgc cgaggagccg
    11161 ggtgcgcgcc gcgtcgcgtt ccgcgaagtc caccgtgtac tcgacggcca ggacccattt
    11221 cccggcgtcg atgtcgatgc gcagcgagag cgccgacacg ccgggcaggt tgcgttcgat
    11281 ggacgcgcgg atacgctcca tgggcgcggc ccagtcgcgc gggggcgtct cgagcgggat
    11341 ccggatggcg tcggcaatga cgccgcgacc gaagttgacg atgggatgat ccagcagggt
    11401 cttgttgggg atacgcacca cgcgctccgt gacgtccggg cagggccgca ccgacaggta
    11461 catggtgccg atgcggctca ccaggcccgt ggtgtcgccg atgcgaatgc cgtccccgat
    11521 gcgcaccggg tttttcagga tgatgatgat ccatacgaga tacgacatga tgagctcgcg
    11581 catagccagc gcgatcccgg tggccaccag gcccacgaag agggtgaggt tctggaagga
    11641 caaccccagg tggaccagca ggaataaaac cgggatgagg tagagcgccc ccttgtacag
    11701 cttggtgagc agaatgcgct gctcgatctc cacctcgccc tcgaggaagt ggaacacgcg
    11761 gttctcggtg aaacgcagga tgacgttcac caccaggaac ccgatgatga cgatcgagag
    11821 cgtccgcgcc gccgtgatga tctccggcgg gacgggatag aaccccagca ggttgaccag
    11881 gtagaccgct tcgtccatga acaggatggc gatcaggagc gcgatgcggc ggttgatgcg
    11941 ggcgttcgcg ttgaagggca gcttcatcat aggtctttga agagcgattt ttcgacgtcc
    12001 gcaatgcagc gatcgagacg ggttttgtcg atgaggctca cggtggcgcg gtcggtgaac
    12061 gaggcggtga acaggagcgc cccgccctcg aactcccacc ggccgatgta catctcctcg
    12121 ttcgcgtaca ccggctcgcc cgcgacgctg cgcatgaagc gcaggagatc gacgatgtat
    12181 cgctgatcgg cgtccggccg atagacgacg atctcgtagg ccgcatcggc gttgtcgaag
    12241 gacacgcgga tctcgctccg gtcggcgagc cgcgcggtga cgatcgcccg gccgttgcgg
    12301 catttccccg gggccgtgtc ggcgccggga cgcgcgccga gccgtgcgag cgtggacacc
    12361 acggccgcgc gcgtatcgcc caggcgaaac tccgcgaagc ggagcgtggc cggggccgat
    12421 cccgcgcggg ggacgaagag cgccttgaag gcgtcgtagc gcgtgcgcag cgattcaccc
    12481 tgcatccgtt cgtcggccgc gatgtcgcgg taggtgatgg cgacgccggt cgcagtgtac
    12541 tcggcgttga tgccgtagcg cctgttctgc agccgccaca ccgcgcgcca gatgaacccg
    12601 cggttcgcgc cgccgctctc gacggtggtt ttcccgtact tgttctccag gaagttgagc
    12661 agcttgctca ggtagatgga gtccttgtcg cgccccaaca gcgtggtgat ggcgtagagg
    12721 gtcccggcgg cgttgaagcg caggatcacg cgctcgttgt tctgctcgtt ctgggtgatg
    12781 gtgatggcgt tcgcggcggc gtcgtgctgc gcgtccatgc cccggtagga ggccgcgatg
    12841 atgccgcgcg cctcggcgat cgactggccg aggcggaact cgcggtagcc gaggccggtc
    12901 tccgccgccc ccgcgccggg gagtgcgagc atgacgagca agcagccgac ggcggcggta
    12961 cggcccgacg ggcgggcgcg ggtgtggttt cgagcgcgca tacgcggcca gcacagcacc
    13021 ggcatcataa aatggccata atttttttgt tgatattcca tgacgctgaa tatttactca
    13081 actcgggtcg atactatata tagacggcgc gtcaggcgga atagaccatg atcaacacct
    13141 cggaactcac cgaccggctc atcgacggcg ggacggacat caccctcgca ttcaagcatc
    13201 ccgacgatcg cacgatcatc gcgatctcgg cgctggtgtc caagatcctg tcgcggctcg
    13261 atcgcgtctt cttcatcgac tcgctcatca tcatcgtgaa ggagatcgtg ctcaacgcct
    13321 acaaggccaa tgcgaagcgc tggtacttca gctcgcgcgg gctcgatatc gccgatccgg
    13381 agcagtacgc cgccggcatg gcgaggttcg atcacgaggt ggtccggaac ctggagcgca
    13441 tccgcgacga tatggagcgc agcgactata cgatcaccct ggtgatcagc aagcgcccgg
    13501 aggggatcat cctgtcgata accaacaatg tccccatcct tcccgacgag ctggagcggg
    13561 tgcgggcgcg cctccgcaag gccggcgact tcgacaacct gcgcgacgtg tacacgtccc
    13621 tctacgacac caccgagggc gccgggatgg ggatcatcct gtcgagcctg ctcttgcggc
    13681 attacggcat cgacgcatcg agcctgaccg tcacccccgg agagaattcg gtgaccacgt
    13741 cccttacggt gccctacgag ttccgcccgc gcgaggtggt ctctatcatc cgcgaccgga
    13801 tcgtcgcggc gatcgagggc ctgcccacat ttcccaagaa catcgtggag ctgcagggca
    13861 tgtgcgccga cccgtccgtg gccatcgagg ccatcgcgga acgcatcacg cacgacccct
    13921 gcctctccgc ggagatcctc aaggtggcga actcggcggg cttcgtcacg cgcaagcgca
    13981 tcgagacggt ccacgaggcg ctcatgatca tcggcatcaa gaacctgcag acgatcctcg
    14041 tggtgaccgc ggcgcggaag atcctggcgc agcgctatca caagttcgag gagctgtggg
    14101 agcattgcac caagaccgcg ttttacgcgc gccggatcgc gcacgcgctc aagctgggcg
    14161 ccgcggccga ccacgccttc ctggccggcc tcctgcatga catcggcaag atcgtcctgc
    14221 tctcgaccga tctcacgctc gtcaaccaga tcgccgatct cgtcgccaac cgcaaggtgc
    14281 gcgcctcgac catcatcgag gaggcgaccg tggggatcag ccatggcgcc atcggggagc
    14341 tcatcgcccg gaagtggaat ttcccggcgt tcctcatcga ggcgatccgg gatcatcaca
    14401 ccccgctcaa tgcgcctccg gagtattccg tctcggtgtc ggtggtgtac ctggcgaaca
    14461 tgctggtgaa catcgaatcg aacgactgcg cgtattcata cgtcgagacg caggtgctcg
    14521 agcgcttcgg gatcgccggc gaggacgcct ttaacgccct gcacgaccag atccggaccg
    14581 aatacgccga gcattcggcg gcgatgaacg cgtaggcagg ggccaatccg ctcagaccaa
    14641 tttctcaatc accgcgtcga aatcgaaatt gctctcaacg aacgctaaaa actcctcatc
    14701 gctcatgagc gactcgaaca cgaccgggtt gaagatgtcc gccgtggcgt cttccttgcg
    14761 atagatgaag gtcaggtttg cccgatcgta tgaaagccgt tccagcccga cagaacgccc
    14821 gtccgtgtac accttgccgc tcttgaggat ctgcgcgacc gtatgtgcct gatgcggttc
    14881 catgatagag tgcaccgttc cggtattgtg actgattctc aaggtaaccc cggaaacggc
    14941 gcatttcaaa ttttttcgcc gccatcgggc gttttcgcga gcccgtgagg cgtcagtcct
    15001 cctcccgcgt gcgctccttg ccctggatgt agccccaatc cttgagtatc acattgatgt
    15061 ccgccgagag gtacttctcc cgcttcacct cgcggacgct gctctttgcg ccctcgtgcc
    15121 agaagagcag ggcgcgtccg gcgagcgcca gcatggcggg ctccgggcgg ccgtgcagcg
    15181 cgcggtcctc gccgttgatc gcgagcgtcc cgccgagcga gcgcgccgca agcggcagga
    15241 ggttccgtcc gcagaggagc ggcgtcgccg gtgcagcggt gatcgcgacg cgcgccgctc
    15301 cgggaatggt ctggaaaacc agcgagccgc cggccggcag cgagaactcg cattcccgcc
    15361 gggaaagcgc ccgcgaagag gaacccgcgc ccgtggtcgc gattgaatag aaaaacccgt
    15421 tttcgatgcg gacccgtccg cgcgcggggg cgccgttgga gaagagcaca ttgtccggct
    15481 gcttgaagcg cgcatggcgc aggagcgcgc gcatccggcc cgccgtctcg ggcaggtcgg
    15541 cggccagatt gcgcgtctcg cgggggtcgg cggagaggtc gtagagctcg gcgtattccc
    15601 cggcgtactc cccgccccag tgctcgggct tgaggccgaa gcccgtgaag cgctcggcgt
    15661 atttatagcc gccgtaccgg accgagtaca tccgtcgccc ctcagagtat gcgatgcgct
    15721 cctgtcgctc ctcgccgcgg accaggggaa aaagcgagcg gccctggaag gagtccggcg
    15781 gggagaggcc cagcgcctcg agcacggtgg gggcgatgtc gatgctgcgc acctgcgcgg
    15841 cgacgcgctt cccggcatag ccgtacgcgg ccgggaactt tacgatgagc ggcgtgtgga
    15901 gctcctcgtc gaactgcgtc tgcccgtgcg tgtagatcgt gtcggcgccg gtgaaggggc
    15961 tcgacccccg gtcgtgcgaa aagatctcgc cgtggtccga ggtgacgatg atgagcgtgt
    16021 tttcgtagag ccccaggcgc ttgagcgcgg cgatcacgcg ccccaggccc tcgtcggtgt
    16081 aggcgatctc cccgagatag gcgttgaaca tggggtcggc gccggggaag cggcgggccg
    16141 cctcgcgata aaagcgcgcg ggggggctgt agggattgtg cggggcgttg tagttcataa
    16201 acagaaagaa cggccgccgc gcgttctcgc cgagccaggc gacggtctcc tcggtgatgt
    16261 cggtcgtgtc ggccaccgag gtgctgtatt cggagaggtc cgccaggtcg caatccacgc
    16321 cgatgccgct gtagtcgatg atgaagggat tgttcccgat cgcgcgggtg agatgcccgg
    16381 cgcgcttcag gagcgtgggc agcagcgtca cgccgctctc gcggtagaag aaagcgcgct
    16441 cctcgtcgtg cacggccggg tagaacaggt ttacgccggc tgcggaggcg ctgcacgagc
    16501 ccagcatggc gatggtggag gggcgcgtcc agttggcggc gacgatgtgc cgctcgaaga
    16561 gcacgccgtc gcgcgcgagg acgtccatga cgggcgtgac ggcgtagcgg tccgcgagcg
    16621 cattgaggca atcggcgcgc atcgcgtcca cgatcacgaa caccacgttg gtcggcgcct
    16681 cgcgcgtcgg cgtaacgatg cggggcccgc ccacgaagag gtgctccggc cgatctgcgt
    16741 cgctctcgaa gcgcagcgcc agtgtcgcgt gcgcgccggc gccgcgggga agcgcgatcg
    16801 cgtgcccgcg ccagcgggag tcgggaattc catgttcgtc ccgcgccgaa tcgtacaggg
    16861 ggatggaggc gccgccgtgg gaaaatgtga tgatgagccg ccgcgcggcg cgcggcgacg
    16921 cgcgccggat cgacgccagc gaaaattcca gcgtgccgtc gcggacgggc ccgtcaccca
    16981 gcccgatgcg catctcgacg ggcgcgccgc ggtgcgccga gagccatatc gactggcgga
    17041 tgcgctggtg cttcttcgcg cggtccatga cgaaacccgc ggtgacgaga tacggcgagt
    17101 cggggcccca gggcgtggtc gagagattgt cgaagcgctc ccggtcgcgc cgcttgacgg
    17161 cggcgtccgc gtcctcgatg cgggcgtcgg ccatgcgggc gcagagatcg acggcgatgc
    17221 gctcacggac gtcggaggat gaatgcgcgc cggagcaggc gatcatcatc acgatggggg
    17281 ccgtctggag gatgtgcgcg agcgtgcgtg tggtcatggg cgcgtccggt accgttggga
    17341 ccattcgtgg ggggcgtcga taaatggcaa atacaatatg ccgacggccg gtgtgtcgcc
    17401 cacacggggc cgaaaaagaa tattgacacg gatgcggaca cgcgggtacg atcgtgctgg
    17461 gccgtacagg aatgcgcggc cgacgaccgg tgcggaggat tgcgatgaaa cggacatgta
    17521 cctggctgat ggcgctggcc gcggcgacgg cgatcgggtg ctccaccata gacgttgcgg
    17581 tgttccccac cgcggagttc cggcagcagg tgaagaagat cgcggtgatg cccttcaatt
    17641 tcaagggaca ggacatcggg caggatttcg ccggagcgat ccgcacctat ctcatgcagg
    17701 aggcgccggg gcgctttatc atcgtcgagc gcgaggagtt cgaccgcatc gtggacgagc
    17761 agaagatggg cgccacgggg ctgttcgacg aggggcaggc cgccaagctc ggcaagatga
    17821 tcggcgcgga catcatgatc gtggggcgcg gggacgtgct cgagctcacc ggcccgggca
    17881 cctatccggg caagcacatt cacacctgcg agatcaaggt ggtgagcgtg gaaaagggat
    17941 cgctcctggt gtcccttatc aagacgccgg ggatcgcatg ggatatgggg taccgggcca
    18001 agtggtgttt ctcgcccttc ggcgcgggca tctggaagga tgcagatctc gtcgcccaga
    18061 gctcgatgta ctcgaacctg gcgcggcagt gcgtcaagcg catcctcggc gcgatcgagc
    18121 gcgtcgagaa acggccgctg ggatactgac gggcgtgcgc atgtcgcgta tcatgaatgg
    18181 cgttcgcctc agtacggtgc agccgacccg acgcacggaa cgcgccgcgt gatcatgggt
    18241 acgatccgga cagcagtcgc gcgtatcgcc gccgcgttgg tcgtcgccgc tgtcgccatc
    18301 accccctgcg ccgcgatcga gttcgatgag ctcgacaagc ccccggaggg ggcccacaag
    18361 gggcagatgt ttctcggggt cgtcatctcg gtgggcgcgc cgtcgggcga gctcgtggac
    18421 gcggaaacgg ccttcgtgga cgggtccacc tataccttcg aaaacgaggt gaccaagcag
    18481 ctcaaggtgt cgcacctctt cttcggcgtg ggggcatcgt tcgaatacat gcccatcgac
    18541 cacgtgggcg ccaagacgcg cgcgatgcaa accatcgtgg tgcagcgcac ggagttcggg
    18601 accgactacg agaactggcg ggagaacctt tacacctgcg gtgcgatcta cgcggggccg
    18661 gcctttcatc tcaccacgcg gcggagctgg gacgtgacgc tcgcggcgct ggcgggctat
    18721 gggtggggcg agtacgcggc gacccccgtg gccaagaagc tcatcgagaa ctatacgggg
    18781 gaaaataaaa aaaccgcctc gagcttcacc tatctggtgg agctcgcctt ctgcgcctac
    18841 ttcagcggcg ggctgtactt ctcgatcggc ggcgagttca tatcgtacgc cgtggacttc
    18901 ggcaagccct tcgagctggt caatccccag acgcaaaaga aatactatga agggaaaagc
    18961 gccggcgcga tcacctcggt caactgcgtc ctctcgatcg ggtacgcctt ctcgaattga
    19021 tcgctccggt tgcaggccgg cgcaatcaga caggcatgtc gttggatagg tgagtaaaag
    19081 cggctgtccc catcttcacg gagcgctcct tcctcgtttg cgttacttct acaggatctc
    19141 gcagccgatg acaaccgaat cgtcctcggc cggcgcggcg ccgcgaaacg acgcaatcgc
    19201 cttctcgacg cggtcgagtg cgtctgaaag ctccaggtgg gcgaattcgt caattaagga
    19261 cagcagccgc acgtacccga agagctcgcc atcggcagtt cgggtttcga cgatcccgtc
    19321 ggtatagaaa aacacccggt ctccagtgcc caattccaag cgcatgtcgc agaacgttgc
    19381 atgtgcaaaa tgaccaatca cgggcccgtg gcagaccagg aattccgccg gccttccgct
    19441 gcgttgaagc accggttccg gatgccctcc cttggaaaag gccagcgata ccgggccctg
    19501 tcccgtccag gaaaaaaacg cataggccgc ggtaagataa tgtccgggca tggctccgag
    19561 gagattcaga ttgagcgtcc cgatgaagcc cgcgggatcg cggtggctgg tgcggaaaat
    19621 ccgttccgac tcggatttca gcagggcaag gaacagcgcg gccgatacgc cgtgccccga
    19681 aacatcgcca atgagcaggc ccagcccctc gcttgtcgca gcgaaggaga aataatcgcc
    19741 ccccaccgcg tccatgctgt tcgagcggaa atccgtgcgg acccgttccg ttatcggcgc
    19801 cgattgcggc aggagggccc gctggatgcg ctgggcgtgc aggagctgtt cctcgatttg
    19861 ttcattgcgc gcgctgagca cctccctgct ctgtcgaagc tccgctgcgg tcgctatgcc
    19921 ctgttcaatc tccctgcgga ggagtatctt ttccgtcagg tcgtgggcga tgatcagcac
    19981 cgcgatgatg tctccctccc ggtcgtgcat ggcgctgccg ctgatgcgat gggggacctc
    20041 cccgccgctg cgggtgatca gcgcgtcctc gagggaaaat tcctcacgat ccccggcgcg
    20101 tactgcgtcg aacagagtgc gaaatgcatc gcgattccgc aggaattccg taaagggccg
    20161 cccggcgagc tcggtctcgc tcgatcccag cagggtcccc gcccaggcgt ttaccttcaa
    20221 caccgttccg ccgggatcga gaaggacaac aacgtccctg atgcgcttga ggatttcacc
    20281 tgaggcgaag gccagggaaa gcgacagcag ctggtaacgc acgatggtga agagcgtgcc
    20341 cgcggcgagg agaacgatga tcagcggcga gagtgatggc atggcgtaca cgccgataag
    20401 cggaagcagg atgtcggata taaaacaaag taaaaaggtg aaggatatgc agatcgtgag
    20461 aatggccgac tgctttcgtt cgcgccgcaa cgggcttcgc aatccccagc agacaaggat
    20521 gatgagggcc acggaaatgc agagcacctg atagaggatg aagaaccagt accacggtga
    20581 atccgtgagc ggaatctcga tccagcccag cggatggcga tatagcgcaa agccctgaaa
    20641 atgtcccgtc cattgcatgg ctaaaagcag aatcccgggc gcgtacagcg ccgttacgaa
    20701 ccagggattt ctcgttaaac cggatccggc aatgtataat aacacatggg cggccatgcc
    20761 gctgtagaga caccatgcga gtgcgctaat agtgtacgcg gcgtatgcca tttcgggcgc
    20821 atggaacgcg tgcatcaccg caaacgacgc ggcccaaagg ccgaagaggg cgcacaggag
    20881 cgagaagacc cgattaaggc cgtcccgaag ccccctgctg aggatgaacc cggcaaggta
    20941 gagataaaag agcgatgcgg tcacatgaag aaggatgaaa acattcatgt cgcaagaccc
    21001 gcgtcgttaa aaaattgcag gacgcgcagc gtcgtccact ttcgcgcctc ttcgtcgtcg
    21061 atcgttcgcc ggctcccgaa caacatcccg ctggagaagc gctccatggc ccaggtgccg
    21121 tcggcgtttt gcttctcgat gagcgtattg aacgcctcgc cgctgcggct gtcgtcgatg
    21181 cgtccggcgc ccgcgacgac cgaaagcccc gccaggatgt cgtagtgcgt catgagcgga
    21241 tagcccaacg acgaccattc gtgcagcccg ccgcgctcgc accgcgacgg aatatcggag
    21301 cggttcggcc ggacgaagca gcgatgcgcg agaaaaaatt ctgcggcgcc gtcaagggcg
    21361 ccctcggggg cgcgcccggt cgtccgggca tggcgcagca atgcgtcggt gcaggcatgg
    21421 gtcgcgatgg ggcagctcgg cacggccgta cgcccctcgc ggcgcaggcc tccacccgag
    21481 cgtcccagaa ggaccagtcc cagcgcgtcg cgccatccct cctgcgggca gtgcagccag
    21541 ccgccgtcac tgcgctggcg ctcccggatc cacgaaagtc cacggagcac gcgcgcgtca
    21601 tcggcatagc cggcttcgat gagcgcgcgc agcatgtcgc cggtccggca ggcgagggcg
    21661 atcgcggggc gccaggcgag cgtgaatcct ccgtccggta gctggcatcg ggccagaatg
    21721 cgctcggcgg tgtcgcggac gaaatcgtcg cgccgatcga aacccagcgc gaccgcctcg
    21781 atgaagcgcc acatcatccc gctgccgtag aggtcgaagc gccgcgcatc gcccacgacc
    21841 ccgccgtgta tcgcgcgcct cagtcgcgcc gcatgcgccg tgccagacag gcgttcgcgc
    21901 gcctcgcgca gttcggcgtc atcgtcggtg agccccacga ggtcgcggag cgtgcgatag
    21961 cggagatgcg gatggcgcgc ctcgagcagg agcggaatcg gatcggcggc gaggtagcgc
    22021 gaggccgtca ttgctcgtac tcgtaggtca agggggccga tcggtttccg gcgcgatcga
    22081 cgatggtcat cgtgaccgta tgcgcgccct tggcccagat gggattgtgc ggcaggatca
    22141 ccacggcccg gtaatcgacg tcgaaatacc agaagacgct cttcccgtcc accgcgaggg
    22201 aaatcttcga caggtccacg ccgcttcccc ggtcggatac ggtgaagcga agctgcgtgt
    22261 tcggccgcgg ccgtttcggg ttgcggtggc ggatgaccgg cgggatctcg tcccgcgcca
    22321 gcacatagcg gcccatgcgc ctcacggtgg cgtaaaaggc gcgctccttt ggatggtagc
    22381 tgttcgacac gaagccgatc ccggtccccg ccgcgttcac ggaatagatc cccaccgtgc
    22441 gatgatcggc gccgtcgtag gcgatgcgta tgcgcgccgt cgaatcgatg cacaggtgcg
    22501 tgggcgttac ggcgtagcgc gcgctcaaaa cgggaaggcc cgagagaacg ttttggggaa
    22561 ggggcgattg ttccatggcc accatttccg ggtagagcgc cgctgtatcc gggaaggaga
    22621 gcgtacagcg cccgtcggcg ctcgtaagga caagggccga tcccgggacg aggttgtggt
    22681 cgaaccccgg cgcgggttcc ggtgccggcg gctccgcacg gcaggagaac tcgatgatcg
    22741 agcggttcag gcgggcgtca aagaactcgg cgcgggcccg gcgcgtcccc cccggcgcac
    22801 cggcgtttat cacgccgtcc ccgctggtgc gcgcatagag gtaaaaggtg aaatgccgcg
    22861 agttcgaggt gtcgtaatcg tagatgagtc ccatgcggtg ggtgtaggcg cggacgatct
    22921 tgtcgaagaa aagccggtgc cgcttgacgc cgtcgacgta ccagttgaac ccgtaggcgg
    22981 tgaccttgtt ccgccagccc acggtgtcgt ggacgcgcgc cttcacgccc acgcgccccg
    23041 cgatgcgggg aacgacgttc cccgccagga cataacggcc ctctttccgg ttgacgagcg
    23101 tggcgcgata ggccttttcg ccgggcgtgc cgtccacacg ggaaaagcgg tcgagcggcg
    23161 tgaggtagac cgtttcgatg accggcgcgg tggcgtccgg gacccacagc gcatagcgaa
    23221 gcgggttgag gtggacattg ttcttgtcgc gcacctcgaa gtggaaatgc tcgagccccg
    23281 cgccgctgtc gcccgagtag ccgatcgggg ttccccggcg cagcggaatc tcgggcttcg
    23341 agaaatcgac gcgaaaatcg cggtgattgc ggatgtgctc gatgtgccgc cggacccgcg
    23401 cattggcgag gatgccgtcg gcgaagcggt ccatgtggcc gtagaaggtc gtgcggccgt
    23461 cgggatggcg gacgaatacc gcgtacccga tcccgtaatt ctcgtacatg acggcgctta
    23521 cgtagccgcg ctcggccgcc acgacgcgga acccgcgttt gccgccggtg ctcacgtcaa
    23581 gcccgtgatg aaaccgcgtg gagcggaact cgccgaaggt gctggtgacc cgcttgggaa
    23641 catcgatggg ccagcggtag gattgcgcgc cggcctccgg cgcgctcgag acggccagca
    23701 gcgccgcgga acacacaatc gcaaccctgc gagcgatcat gccgggcggc tgcgatcgga
    23761 tgcggagcat gcgcggctgt cggctacttc ttggtcagct tttcggcccg gctcaggttg
    23821 tcgaagatgg ccgtgttttc gggggccagc ttctgcgcct tccggtaggc ctcgacggcc
    23881 tcggcgttct tgcccagcag ctcgtagctg agcgcgaggt tgttgaggat gatgccggag
    23941 gacggcatga gctcgtgcgc cgtcgcgaac gcggtgttcg cctccgagta ccgcttgagg
    24001 cgcatcaggg ccatccccag ggcgtggtac gatgcgtaca gggccgggtc catcgagatg
    24061 gatttttcga gcagctcctt ggcgcgccag gcgttgcggt tcgccagcgc catgcggccc
    24121 cagatgtagt aaaacatcgg atcgtccttg atcttccggc ccttccggag gaaggaatcg
    24181 gcctctttca gtttccggtg ctcgacgagc atgagcgccg cgtcgatgta cgggtccggg
    24241 gtggcgcggt cgatctcggc gcatttcctg aagtgcgaga gcgcgccctt gaggttcccc
    24301 ttcttccagt gggcatgggc cgcgagaaag cgcaggcgat agttgggtga gccgggctcg
    24361 cggtcgcgct tcgcgtccaa ggtgtcggtg atggttttga gcgcgtcatc gtacttgttc
    24421 tgttggaaca gttttaacgc atcgtcgtag ctgtcggcgc tcgccggcag ggccacgcac
    24481 aatatcgcac ataggagcag gatcgttttc atctcggttt tcctggggga atgatggtcc
    24541 ggcgccgatg cgtacatcgg cgccggactc ctccgttact tcttgccttt tttcttcgcg
    24601 gtttttttcg gcgcggcctt cttcttcggg gccggtttct ttttcgccgg agccgccttc
    24661 ttcttcgccg cgggcttttt cggcgcggcc ttcttcttcg gcgccgcttt cttcaccgcc
    24721 ggtttcgcct tcggcagggc ggcgccgcgc accgccgcct gggccgccgc gagacgcgcg
    24781 atgggcacgc ggaacggcga gcagctcaca tacgacaggc cgatcatgtg gcagaactcg
    24841 accgagctcg gctcgccgcc gtgctcgccg cagatgccca gcttgatgtc cttacggacg
    24901 ctgcggccct tttcgacggc catgcgcatc agcacgccca cgccgtcgcg gtcgagctgc
    24961 tggaacgggt caaccggcag gatcttcttc tcgacgtaat ccggcacaaa gccgccgatg
    25021 tcgtcgcgcg agaaaccgaa ggtcatctgc gtcaggtcgt tggtgccgaa ggagaagaac
    25081 tcggccgtct tcgcgatccg gtcggcggtg atggccgcgc ggggtatctc gatcatggtg
    25141 ccgaacatgt gttcgatctt cgagacgccc atcttcttgc ataccgcggc gtggaccgcg
    25201 tccaggagct tcttctggtc atccagctcg ttcacatggc ctaccacggg gatcatgatc
    25261 tcagggacga cctttttgcc ctctttgttg agctcggccg cggattcgag aatggcgcgg
    25321 tactgcatct cggagatctc ggggaaggtg acgcccaggc gcacgccgcg gtgccccatc
    25381 atggggttcg attcgtgcag atcctcggcc cgcttggtga gccggtccat gccgatgccc
    25441 agcgccgagg cgagcttctc gcgctcgtcc tggcgggtgg gcacgaactc atgcagcggc
    25501 gggtccagca gccggatggt caccgggagc gggctcatca ccgccagcgt gctcttgatg
    25561 tcgcgcttca cgaagggaaa gagctcgtcg agcgcggcgc ggcgcttctc ctcggtgtcg
    25621 gagaggatca tcttgcgcag gataaacagc ggctgctcgg agttctttcc gtagaacatg
    25681 tgctcggtgc ggaagaggcc gatcccctcc gcgccgaact cgcgcgcctt ggcggcgtcc
    25741 tcgggggtgt cggcgttggt gcgcaccttg agggtccgca tctcgtcggc gatcttcatg
    25801 aacttttgga agcgcgggtt ttcggtggcg tccatcatcg ccagcgcccc ggcgtaaacg
    25861 aagccgcgcg tgccgttcag ggtgagggtt tcgccctcgc ggaaggtgcg cccgtcgacc
    25921 gtcatggtct tcgcgtgcac gtccacgtgg atggcgcccg cgcccacgat gcagcacttg
    25981 ccccagccgc gcgccaccag cgccgcgtgc gaggtcatgc cgcccttggc ggtaaggatg
    26041 gcttcggcca cgtgcatgcc gtggacg
//
LOCUS       DAORHF010000027        26058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1164_length_26058_cov_7.028612, whole genome shotgun sequence.
ACCESSION   DAORHF010000027 DAORHF010000000
VERSION     DAORHF010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 26058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1164_length_26058_cov_7.028612"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4716)
                     /locus_tag="PKJ16_04075"
     CDS             complement(<1..4716)
                     /locus_tag="PKJ16_04075"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HNV46196.1"
                     /translation="MSLLRHIRRIVPLAAVVPALCLFSCVNMKDGGVAATSGPGSSTF
                     TPEAIKASPLSVSVEGASDAPTRWNLFDGNTHTAFAPAEAASLRVDLPRNMKITRLRV
                     YGKSSYDVRVYRIEGANRSASPALTIRGEGLSGSWNTITPPEPFSADALVFAFAPVGE
                     PALGVREIEIWSGESGEIPDEGVCSTLDAVKTPERLAAAISHGYPHIHELSAAPAELS
                     IAANGGREHPGTMSFALAMPPVQVRRAFLRFKGKNLCAAPSVERRINGLSWSGGFPVP
                     CDASDEWKEYFEEINPAWLAAGTNTVDFCSARDVSIKELSVVLVTGSGWNWISGVSER
                     NAFDFDTQTSAGVTIGHDMRIDFDSTVEPEHLLLHVPGPAHALARVQYLNNGSWHDIA
                     GVWNVNCAGLVRGWNRLAFPAPVAADGIRLVFSAAPGGPAAPVEINEVRVAASPIAPR
                     NAVRIVFDYPRNGEFFARSAYIRGFVATTAAISSLSLEGKTANADQTAPADGSFNVTL
                     SKDETRYGAQKDDEPWDPALTALTGIGSFSSALHLYVNRLSSSSGSGSGSGSGPGSGT
                     DGDGEQSDGSYRATLYPDQSKTILFRDVTLKFPAGSVTKTTVIRIVPLSQGEIPKLGE
                     GMINVTYPAAGYRFLPSGRFEKPVEVYFRYTKDAMKAGQKDEDVFMYYYDVADGKWKR
                     LERLAVEPAITMVTSQTDHFTDIINATLVVPEHPDPLSYNPNSIKDIKAGDPGAGINL
                     IEPPTANSNGDVALSYPIEIPRGRNGMQPQIAVRYNSSGGNGWMGLGWDIPIQSVVID
                     TKFGVPRYNGSETYLLEGASIVPIGGNQYRQRTEGPFKRIMRRGDGPATYYWEVTDKN
                     GTRFIYGQSAGSRLASRNGNIFQWCLERVVDTNGNTIDYNYEIDNLGGGGSEQSVQIY
                     LNRIAYTGAAGAPGPYSVEFFHDDGNRRDRIINCRGGFKMLTRFLLSSIEVKYNGSII
                     RKYVFTYREGVFYKNLLSRIVQYGEGGSVYFNEHTFDYHDDVTEDGDFLGFTPEDETW
                     DNSTPGETRGSRSGVHAYIGFAPPFLTKGISAGMKIGSNWSQQETTKSLIDIDGNGVP
                     DYVYLSGNQVHYSPNLAGPDGENRFGNDYQASGLFRNLGKEKSNGSTRGAQLFVTLPI
                     FEMLSVGAFVDRNEGESNSDRYFTDVNGDGFVDFVDGGTVLFNNGNGEFSNTPPENFD
                     QGSANISGIDFGEFPSRAELAADFYRDDPIRMWKAPYSGNVRISGTVQLVQKPLEAGD
                     YLTNDGVRVSIQRNASVLWSSVIGPTEYTPVEPDTVDLVWVDRGDCIFFRVSSVDDGS
                     YDTVVWNPVIEYDTAFPGMVDENGSPVYRYEAASDFSFAGNETYWPVPLSGTVVIDGV
                     VRKQGITSDDIEILIKKNDDIIYRHTLAWDFVGEHPIEETSGVLGVNPQDPSLSGERL
                     LFALHSDTPVDWTQLAVQPRLTYTSRDDGGQVQDDSGNPILFFLLPVSMNLYPEKDPE
                     PVPPFIAEKAGRARIVYQVLVPRYHPDFPDIPILPGSPNYNDPGVSDAIAADYTARVV
                     LAAKKDGGLVFKQAGVIEHG"
     gene            complement(4713..6107)
                     /locus_tag="PKJ16_04080"
     CDS             complement(4713..6107)
                     /locus_tag="PKJ16_04080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV46197.1"
                     /translation="MGKADSPTNSISGSVTMYGRPVQAASVALTHESYGGVGNVSTDA
                     NGVYTFSDVREGEYTLAFAKAGYTFDNPLVRITVGGGGVTLPENVSVISWKNAYGGSG
                     DEQAYAVRPTPDYGCVVAGFTGTGSSGVSDVLVKKLDLFGVQEWSYASGGPGYDVAYD
                     IERTLDGGYIVVGEYENGLNINDFYVLKLSGGGEKEWERLYGFSERDVARSVRQTSDG
                     GYIIVGSRESDTPNRDLDNYYILKLDTNGNLGDPGTWHKEYGGESWDVAYAVEETGES
                     YVIAGSAQIDLLDPSRSAILVRWLRKSDGVDIYNKIYSLGDSSEARAMVKTPDNGFVI
                     AGFTSDLLTGFSDLCLLGIDDQGNELWRRTFDWSFNDRAASISNTADGGFIVAGHTYT
                     NVTDSYSFLVMKITASGDETWRRVYSFSEDDRAHSVRQTPDGGYIVAGKTWSAGAKDN
                     MMLLKLDREGNIRE"
     gene            complement(6810..7991)
                     /locus_tag="PKJ16_04085"
     CDS             complement(6810..7991)
                     /locus_tag="PKJ16_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46198.1"
                     /translation="MAYRITAYIVLATCLLAVAARGAPSRAPLRGSHDRLTFLVFDAA
                     AVRSPRALARGLSAALYAELAASREFTLVNEPEIAALLKERREPHGPCRGRDCAVRIG
                     GVLGSDKTVTGTLQLDTIEDTEPVSRYVYRVAKKERWRLTVAVHDTPTGKTELSFTEY
                     PRTRAEIEAKTREIARKIVEYYRKRAWARATAAEPLRAAAVASAAMDASAQARPGGPD
                     APAARWGLEFDSLAASAGYLKPLNAFSNIAHDGFGIAADARWRLVPWRSVVVSLGAAG
                     YRIRDRLESIQSYYLAMALVTAGYSLSPARGLYVVPSLGFGYLGHIIYGDKSIPDGRT
                     RYEYKMHYTYDPSLFAGGELQYALGGRMRLFLAPWYVYFFEKDENGQAVMITAGLRFM
                     Y"
     gene            complement(8432..8854)
                     /locus_tag="PKJ16_04090"
     CDS             complement(8432..8854)
                     /locus_tag="PKJ16_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46199.1"
                     /translation="MGQEKTILLVEDSDRDAELITLALSEIDVPVVVERSRDGDGALE
                     FVANRGCSARDISLVLLDIKLPGMSGLEVLNRLKSDERTGGIPVVMLTSSRERRDIRD
                     AYRMGVNAYVVKPMEFEDLLNALKCVAHFWCVVNERSY"
     gene            complement(8871..11969)
                     /locus_tag="PKJ16_04095"
     CDS             complement(8871..11969)
                     /locus_tag="PKJ16_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46200.1"
                     /translation="MKTILLVGVIDERRIRLEDFLAARGYHALIAANGREAISMARAV
                     PPDIAVTDILMPVMDGFTLCREWMNDEILSKIPFVFIATADTDPRVKAFALSLGAARV
                     VVKPVEPHFLVSLFEETLGEFPGAASRRPKDGTALADLNYLRDESFPRTLEKKMVEFS
                     DSERRYRAIVENQPVAVCRWRPDTTLTYANEYYFRHIARPGEKEGKRWIEFVPEEHRS
                     AIMEYYREISETPRIATLEHPVYGADGTIRWFSWMDVPIFDDDGRLAEFQSVGTDITD
                     RKEYESKIERAAQEWSTTFDSIQDAVWLLDKDFRVLRANAASVAFFGLSPSDLAGRKC
                     FDIVHGTGGPIPDCPAARVSVSRKPESGEFFLRNRWMQVTVYPIFDESGEPNGIVHVM
                     RDITDAKKIEIALQESESILKRAQRVARLGYWTWKITENRLIWSEVMYGIFGIDKDSF
                     NDALADVIARAIHPEDREAVEKSNRSVIEDGRPSPLEYRVVHPDGSVRWIYAEAGDLI
                     RDEAGVPVELSGIAQDITERKEHELAQRESEERYRLLFEANPHPMWVYDLETLGFLAV
                     NDAAVRKYGYTRDEFLSMTIADIRPSEDVPRLIENIEKVDVGIDYAGVWRHRTKGGEI
                     IFVEIISHVLTFKNRRAELVLANDITERKAAGDLLRESEARYRRLIDTIPHGIAVHQN
                     GIIVFCNPAGASILGADAPERLIGMPITGILHPDGLESARRRIEAMLSGAEGLYPAED
                     RYVRLDGRVIDVEVMATSLNYRGSPAVQVVVTDISEKKRAQKELERLYADLERKVAER
                     TADLEKANKELESFSYTVSHDLRAPLRHINGFLEMLRREIGDSFPPKASRYMDVINDS
                     AKRMGRLIDDLLSFSRMERTGLSVREVNMSHLIDELLKEHKEEIAERSVTIERASLPV
                     VRGDPSLLRVVMENLISNALKFTARVSSPAIEIGYRAAESSHVFYVRDNGAGFDMRYV
                     DRLFGVFQRLHDEREYEGTGIGLATVKSIVQRHGGMVWAEGEPGKGACMFFSLPGDAR
                     GV"
     gene            complement(12035..13453)
                     /locus_tag="PKJ16_04100"
     CDS             complement(12035..13453)
                     /locus_tag="PKJ16_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV46201.1"
                     /translation="MNALSSSAADVNALLRVLILEDSENDAGLIENEIAAAGIPCTTR
                     IVADESSFRNALETYKPSVVLADFRLPSYDGFQALEEVRSQDRLLPFIFVSGTIGDER
                     AIEALKRGATDYVNKEHLGKLPSAILRAIDEREERRQRQRAEEALERIRHRNIIILDS
                     TDEGIAGLDGDLRFVFINRSALAILGYGMKELLGESLDTIFADQANGPGGASAVACVP
                     TPVPGGASIRNLRRADGTTFPAEVTVRTVTDDGSSLGCVISFRDISQRLKSAADLASS
                     FERMRKIMFESIHAMATALEYRDPYTAGHQKRVADLSKEIGSVMGLDDETIEGLYLAG
                     VVHDIGKIYVPAEILTRPSKLSPVEFSMIKEHSEAGYQILKNIEFPWPIAEAVYRHHE
                     RLDGSGYPGNLTGKDIILEARILAVADVVEAMASHRPYRPSLGLEPALDEIAKNRGRL
                     YDPAVVDACSHLFRNRGYRLSQ"
     gene            complement(13450..14319)
                     /locus_tag="PKJ16_04105"
     CDS             complement(13450..14319)
                     /locus_tag="PKJ16_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46202.1"
                     /translation="MKELGAFIKAAREERALSIREAAELCGMSNAYLSQIENGKISRP
                     SPETIRKISGLYQMPFEVLMEKAGYALPEYGFLPKYVPPSRITVLIVDDSPHDREIIV
                     SHLERESARRYDVFFAGTGSEAVEQYRRCDPDCVLLDYRLPDMDGLAVFRKLKADDAG
                     KDVSVIMLTGYGNEETAVQAMKMGAANYLNKNLITPEILVGAIDQAVRRKHFRESIRD
                     IAGNGRQKWEDLHNAVGGIARAASDALARLAEKKPELRHDEDFMALAAQADRLMRLGG
                     SVKNGVAVKGEPI"
     gene            complement(14515..15981)
                     /locus_tag="PKJ16_04110"
     CDS             complement(14515..15981)
                     /locus_tag="PKJ16_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46203.1"
                     /translation="MGANSELKEILYLTRKIKDGSEKKYILARSFYELGGLSYEKGSR
                     KGDYEIRDRVGKIVAEINRYYEHIKLFDNGKNGDIRAYEDILVFFQNSLIDSRLIDDK
                     TSALREEIARQEREDRLHNARHAGTPLERKKNAYVTELEKSLQRFEDQLRTVVEPRLR
                     EFLSDVNHILYCEKLKTIVQNIAKSAGGGAGAARTDDFIRHTITFLWIDRKINGTRVA
                     ASDLRDFVTGVLEQMGLLAPLEERAGLGKRLLAESIAEIGSQLDGLLAVEREKTKPSP
                     PVEVAEQEEKRLEAIRVSTRMKSLLDNLCYVNISKPSLYLPEYRDYRDLAAHKENRYL
                     FHRPGSFEVSLKIVSDYHSNSLMTVYTWVKREVRRDISQLALLRPLIDVLPDCERFVR
                     VYSTALFISSVASNQGVSLVMGEPQQFIPVGLARELHEVVCGNCERVYAAFADTAAAL
                     ALMQRSDRDLLGKKLRIMFDSYSLARSKIVEWSNRLRV"
     gene            complement(16126..16548)
                     /locus_tag="PKJ16_04115"
     CDS             complement(16126..16548)
                     /locus_tag="PKJ16_04115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV46204.1"
                     /translation="MTKERRTYPRLKVIRFIAGLQTVGKGEFEKIEIVDISAGGLCFL
                     QTFPVSVGENVLFSFTFRKCSMQMPGKVIRVDGREIGVKFTDSEDEIMKFVRLFNREV
                     SGMLITKKGTSRIMVPGEYDESMGFDDLNQLLDPDKDE"
     gene            16740..17597
                     /locus_tag="PKJ16_04120"
     CDS             16740..17597
                     /locus_tag="PKJ16_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46205.1"
                     /translation="MKNLGTFIKAARKRTAITIREAEKRSGVSNAYLNQIENGRIKRP
                     SPDILGKIGALYDLPYDVLLHKAGYSLPDFKTDEDAERDRCYRILIIDDDRRDRELLI
                     NRLAGFTGGALAVREARSGEEGIAILEKELFDCIFLDYRMPGIDGLAVLRRIREARPW
                     ETASIIMLTGQGNETAALEAIRLGAANYLTKSENKETILAALKQALLRKQMRRPVAVM
                     PPPRDDEEAPTAHDALTRVRRIRTMVEKIIEADDGNALTEDLRGILREIHAAETALTA
                     ESRQSRAET"
     gene            17599..19551
                     /locus_tag="PKJ16_04125"
     CDS             17599..19551
                     /locus_tag="PKJ16_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46206.1"
                     /translation="MRRRWERTTEIQHTARVAVVSPFPATMTAVHEALAAHQDDTVFF
                     DHAATPQEAIGKNADWRYDVVLIAAGDGPDAVLTALTECLPHFPSAPLIAIGEAVTES
                     MAAAAILAGADDFVSLRDFSRGTLFRAIARAAQHANRKLLRGTSEWRFHLLLESQDDL
                     ICSWLPDTTLLYANDYYCRFYGIGRDRIGTLKWLALVPADMRENVRENYQTFLKEKRT
                     ISYEHEATAADGRVGWQEWRDVPVVDERGEIVEILSIGRDITKRKQAENALRESEERF
                     RTLYSSMEQGVVYVNASGSITDANPAAARILGRSRGHLLGMTLEDPCIKAVEEDGRAL
                     SFGSHPAMIALTTGRPALGVVLGVESAREGTVRWIMIDAVPQFLPGAETPCRAYVTLT
                     DITALKNAKEAMRRFNEELESRVAERTERLAEANRELEAFSYTVSHDLRGPLRHIAGF
                     AEMLAEHAGGSLDDTGRHYLSVIGESVGRMEQIISGILSLTRIGQTELTIAAVDLDRL
                     VRDILISFEPDLAGRDIDIVVCDLPRVRADAVLLGIALRNLIENAIKFTAHTKHAVLE
                     VGHARGAGTDTFFVRDNGAGFNMEYADKLFNAFERLHDTDEYGGLGIGLPTVKRIIER
                     HGGTVWGESGPDGGATFYFTLGAPSS"
     gene            19548..19985
                     /locus_tag="PKJ16_04130"
     CDS             19548..19985
                     /locus_tag="PKJ16_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46207.1"
                     /translation="MTALEKRILVVEDSAHDIEMISRAFDLAGIVACVDTVRSGPEAL
                     DYLYGRNAYEETGAGRPVLVLLDIKLPFRSGIEVVRELKEDENMKTIPVIMLTSSDDA
                     GDIEAAYRAGANAYIIKPTSFSEFVAIIRSMTQFWLTVNEHAK"
     gene            19975..20373
                     /locus_tag="PKJ16_04135"
     CDS             19975..20373
                     /locus_tag="PKJ16_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46208.1"
                     /translation="MRNETVKSDGPVCRQEPFMDGRRAPVRDFPDYYYVYIMTDPDHE
                     LLFVGVSGNLKAVMAMCRFEFEYDHGTVSMEKLVYYEIYDSVESAVMRNRDLHLLSHD
                     ALRHLVNATNVEWADLFEQLDGMAATPGGV"
     gene            20373..20726
                     /locus_tag="PKJ16_04140"
     CDS             20373..20726
                     /locus_tag="PKJ16_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46209.1"
                     /translation="MKIIVVDDDTKTTELLLEYGGLGEEDVCAFHNPVEAIKYVANHA
                     IDIALIDSQTRVIDSLNVIRFIRRIRPSCIVYVFVTEAIDIDYFSVAHHVGAADFIHK
                     PLRESSFSFLAGAEA"
     gene            20794..21117
                     /locus_tag="PKJ16_04145"
     CDS             20794..21117
                     /locus_tag="PKJ16_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46210.1"
                     /translation="MIERACEIIGAHARRIEVNEATRRPDGFDALVLCSAAADSGLIR
                     TIAGVFRGPLLVITETADAVRRPSLYADRTRLIARPTRSIDLANELLRLLNASASATD
                     TAPHR"
     gene            21129..21590
                     /locus_tag="PKJ16_04150"
     CDS             21129..21590
                     /locus_tag="PKJ16_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46211.1"
                     /translation="MIPSILYIGTRTADDELLHHVSERTGRAILRVRALGDLRARNTE
                     CFIDTVIVGTTVELTARVLDEIEHLRGLAEGTVILGLVREGMDDRVDIMIQRRMQPCH
                     ISANSAARAARIVAHMIEERTGVYRNVIERMCNELAHGRMVRTGGLVDKRK"
     gene            complement(21806..22195)
                     /locus_tag="PKJ16_04155"
     CDS             complement(21806..22195)
                     /locus_tag="PKJ16_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV46212.1"
                     /translation="MQYLLVTVTIIRHLCGSGNIGARARDVLDAGPTTPHTFHVSVIS
                     LMEIVYLSEKNRIKINLSRTLERLDASSLYSIVDLTPEIIKKAESIKFYELHDRMIIA
                     TALWLDVPILSSDSRFDDVKGLSVIWS"
     gene            complement(22203..22451)
                     /locus_tag="PKJ16_04160"
     CDS             complement(22203..22451)
                     /locus_tag="PKJ16_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46213.1"
                     /translation="MHSQVVKRIIIKKGINLVPENRLDEVKTYIDTIINQSDGTKPRP
                     VSLQGIWKGTGFEKLPSLEDEIRDLRRDLSSVILKKHA"
     gene            22828..23142
                     /locus_tag="PKJ16_04165"
     CDS             22828..23142
                     /locus_tag="PKJ16_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46214.1"
                     /translation="MSILKGVLDEENKRLKRLLGKYQRDIDALIKGSVSIKDISGQRY
                     AYIAKRKGDTVVFEYIGKASSDKVKRLLEEVNKRKKLTVKIKALKQDIKEIERALGGR
                     KI"
     gene            23126..23812
                     /locus_tag="PKJ16_04170"
     CDS             23126..23812
                     /locus_tag="PKJ16_04170"
                     /inference="COORDINATES: protein motif:HMM:NF023701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HNV46215.1"
                     /translation="MEERYRLFDQTIAALHNAGVLSALVLVGSWSQHLYKHALTNTDE
                     IPLLITSDIDFLIPQPTQMTMPCDVPGILNELGYETATSPVSGHVKYIRRELEVEFLV
                     PEKGRGSDTPRKIQELMVSAQGLRYLNMLQEHTITVDYKGIPVAVVEPAAYALHKFLV
                     SGRRTKKEKMEKDLASAIAMCEYVTHEMCGITTMRDILNSLHPKWKKDILLHIKKHIP
                     ELHDSLISPG"
     gene            complement(24284..25177)
                     /locus_tag="PKJ16_04175"
     CDS             complement(24284..25177)
                     /locus_tag="PKJ16_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46216.1"
                     /translation="MLRLSRGIIKLVRGTGGRFDDYRYYVIARRHFAARSGIFSLAEL
                     CDILHAEYGYASLHRRPGNNRGAVRRRLARLLGSSVLFVPLSDGRYRIRSEKSLLNRQ
                     NSSGPAMWFELPYESVLASRREFYDFCVGVLLAGNKFRANGNIARYSGCTVRRIQYAT
                     ARNHKEGTFRKRYNFLEDFTGSYEEVQKFRAILLNVHGITSPLPRHYKGGEWVARLNA
                     PNTYTSCVLSGVKGDRAQPTGTVVRIGAKEGCWFVPAREPRKSTQLNLFCNPQPVSRW
                     YFNERLYTTDRYIQEHSRLYA"
BASE COUNT         5462 a         7482 c         7648 g         5466 t
ORIGIN      
        1 accgtgttca atcacgccgg cctgcttgaa caccaatccg ccgtccttct tggcggcaag
       61 caccacccgt gccgtataat ccgcggcaat agcatcggac acacccggat cgttatagtt
      121 tggactgccg ggaagtatcg ggatgtccgg gaaatcgggg tggtagcgcg gcaccagcac
      181 ctgatataca atcctcgcac gaccggcctt ttccgcaata aacgggggaa cgggttccgg
      241 gtctttttcc gggtaaagat tcattgatac cgggagcaag aagaaaagga tcggattgcc
      301 gctatcgtcc tggacctgtc ctccgtcatc acgagaggtg taggttaaac ggggctggac
      361 tgcaagctgt gtccagtcaa cgggcgtatc cgaatgtaat gcgaacaaca aacgttcccc
      421 ggacaacgac gggtcctggg ggttgacgcc aagaacaccg cttgtttctt cgatgggatg
      481 ctcgccgacg aaatcccagg cgagcgtgtg acgatagata atgtcatcat tctttttaat
      541 tagtatttcg atgtcatccg atgttatacc ctgtttccgg acgacgccat cgataacgac
      601 ggtgccgctg agcggtaccg gccagtacgt ttcattcccg gcaaaactga aatccgaggc
      661 ggcctcatac cggtataccg gcgatccgtt ctcgtccacc atcccgggga aggctgtgtc
      721 atattcaata accggattcc ataccacagt gtcgtacgaa ccgtcatcaa ccgaactgac
      781 ccggaagaaa atacaatcgc cacggtcgac ccatacaaga tcaaccgtat caggctcgac
      841 cggggtgtac tcagttgggc cgatcacgga tgaccataag acggaggcgt ttcgctgtat
      901 gctcacccgt acgccgtcgt ttgtgagata atcacccgcc tctaagggtt tttgaacaag
      961 ctgaaccgtc ccgcttatcc gcacattccc cgaataggga gccttccaca tccgaatagg
     1021 atcgtcgcga tagaaatccg ccgcgagctc agcgcgtgac ggaaactcgc caaaatcgat
     1081 acctgagata tttgccgatc cctggtcgaa attctcggga ggcgtattgc tgaactcgcc
     1141 gttcccgtta ttaaacagca ccgtgccgcc atcaacaaaa tcgacgaagc cgtcgccatt
     1201 gacgtcggta aaatagcggt cgctgttgga ttcgccctcg ttgcggtcca caaatgcgcc
     1261 cacactcaac atttcgaaga tcggcagcgt tacgaacaac tgcgcgcccc gggttgagcc
     1321 attgcttttt tccttgccga gattacggaa aagaccactt gcctggtaat cgttcccgaa
     1381 acggttctca ccgtccggac cggcgagatt tgggctgtag tgcacctgat tcccgctcag
     1441 gtacacatag tcgggaactc cattgccatc gatatcgatg agcgactttg tggtttcctg
     1501 ctgactccag tttgatccga ttttcatgcc ggcggatatg ccttttgtta aaaacggcgg
     1561 ggcgaaaccg atataggcat gaacccccga acggctcccc ctggtttctc cgggagtgct
     1621 gttgtcccat gtttcatcct cgggggtaaa accgaggaag tccccgtctt cggtaacgtc
     1681 atcatggtaa tcgaacgtat gttcgttgaa ataaaccgaa ccgccttcgc cgtattgtac
     1741 gatcctgctc aggagattct tataaaaaac accttcgcga taggtgaaga cgtatttccg
     1801 gataatgctc ccattgtatt taacctcgat cgaggagaga agaaatcgag tcagcatctt
     1861 gaagccacca cgacaattaa taatccgatc tcgacgatta ccatcgtcgt ggaagaattc
     1921 aacagaatac ggacccggcg caccggcggc accagtataa gcaattctgt ttagataaat
     1981 ctgtaccgat tgttcactac cacccccgcc caggttatcg atctcataat tgtaatcgat
     2041 ggtattcccg ttggtatcca caacacgctc aagacaccac tgaaagatgt tcccgttccg
     2101 actcgctaat cgcgaaccgg cgctttgccc gtatataaag cgcgtaccgt tcttgtcggt
     2161 gacctcccaa tagtaggtgg cggggccatc gccccggcgc atgatccgct tgaacgggcc
     2221 ttccgttctc tgacgatact ggttgccgcc gatagggacg attgacgccc cttccagcag
     2281 atatgtctcg cttccgttgt agcgcggcac cccgaacttc gtgtcgatga ccacggactg
     2341 gatggggatg tcccacccga ggcccatcca gccgttcccg ccggaggagt tgtagcgcac
     2401 ggcgatttgc ggctgcatgc cgtttcggcc cctggggatc tcgatgggat aggagagcgc
     2461 gacgtcgccg ttactgttgg ccgtgggcgg ctcgatgaga tttatccccg cgcccggatc
     2521 gcccgccttg atgtccttga tggagttagg gttgtacgaa agcgggtccg gatgctcggg
     2581 gaccaccagc gtggcgttga tgatgtccgt gaaatggtcc gtttgcgagg tgaccatggt
     2641 gatggccggt tcgaccgcga ggcgctcgag ccgcttccat ttgccgtcgg cgacgtcgta
     2701 gtaatacatg aagacgtctt cgtccttctg gccggccttc atggcgtcct tcgtgtagcg
     2761 gaagtacacc tcgaccggct tttcgaacct gcccgagggc aggaagcggt agccggccgc
     2821 cggataggtg acgttgatca tgccctcgcc gagcttgggg atctcgccct gcgacagggg
     2881 gacgattcgt atcacggtcg ttttggtgac cgagcccgcg gggaacttca gcgtgacgtc
     2941 gcgaaacagg atcgtcttgg actggtccgg atacagcgtg gcccgatagc tgccgtcgga
     3001 ctgctcgccg tcgccgtccg tgccgcttcc cgggccgctg ccggaacccg agcccgaacc
     3061 ggaggaggag gacagcctgt ttacgtacag atggagcgcc gaggagaacg atccgatgcc
     3121 ggtgagcgcc gtgagcgcgg ggtcccaggg ctcgtcgtcc ttttgcgcgc cgtagcgcgt
     3181 ctcgtccttg gagagggtta cgttgaacga gccgtcggcc ggcgcggtct ggtccgcatt
     3241 ggccgtttta ccctccagtg aaagcgagga tatcgcggcg gttgtcgcga caaagcccct
     3301 gatataggcg ctgcgggcaa agaactcgcc gttacgcggg taatcgaaca cgatgcgtac
     3361 ggcgtttcgg ggggcgatgg ggcttgcggc cacgcgcacc tcgtttatct cgacgggcgc
     3421 cgccgggccg ccgggcgcgg cggagaagac caggcggatg ccgtccgcag caaccggcgc
     3481 ggggaaggcg agcctgttcc agcccctgac gagacccgcg cagttgacgt tccagacgcc
     3541 ggcgatgtcg tgccagcttc cattgttcag gtactgcacg cgcgcgagcg catgcgcggg
     3601 gccgggcaca tgcagaagca ggtgttccgg ttccaccgtg gaatcgaaat cgatgcgcat
     3661 gtcgtggccg atggtgaccc cggccgaggt ctgggtgtcg aagtcgaagg cgttgcgctc
     3721 ggatacgccg gatatccagt tccacccgct gcccgtcacg aggacgacgg agagttcttt
     3781 aattgatacg tcccgcgcgg agcagaaatc gacggtattg gtcccggctg cgagccaggc
     3841 gggattgatc tcctcgaaat actccttcca ctcgtccgac gcgtcgcagg ggaccgggaa
     3901 gccgccggac caggagagcc cgtttatgcg gcgctccacg gacggcgcgg cgcagaggtt
     3961 cttccccttg aagcgcagaa aggcacgccg gacctgcacc ggcggcatgg cgagcgcaaa
     4021 cgacatggtc ccgggatgct cccgcccgcc attcgccgcg attgagagct cggccggcgc
     4081 ggcggagagt tcgtggatgt gcgggtagcc gtgcgaaatg gcggcggcca gccgctccgg
     4141 cgttttcacg gcgtcaaggg ttgagcagac gccctcgtcg gggatctcgc cggactcgcc
     4201 gctccatatc tcgatttccc ggacgccgag cgcgggttcc ccgacggggg cgaacgcgaa
     4261 caccagggcg tccgcgctga agggctcagg cggcgtgatc gtgttccacg acccggacaa
     4321 accctcgccg cgaatggtga gcgcggggga cgccgaacgg ttcgcgccct cgatgcggta
     4381 cacccgcacg tcgtaggagg acttcccgta cacgcgaagg cgcgtgatct tcatgttccg
     4441 ggggaggtcg acgcgaaggg acgccgcctc cgccggggcg aacgcggtat gcgtattgcc
     4501 gtcgaagagg ttccaacggg ttggcgcgtc ggatgcgccc tcgacgctca ccgacagggg
     4561 cgacgccttg atcgcctcgg gcgtgaaggt ggagctgccg gggccggagg ttgcggcgac
     4621 gcctccatct ttcatgttta cgcacgagaa gaggcagagc gccggcacga ccgccgcgag
     4681 cgggacgata cgacgtatgt gacgcaggag gctcattcgc ggatgttccc ctcgcggtcg
     4741 agtttcagga gcatcatgtt gtccttcgcg ccggcggacc aggttttccc ggcgacgatg
     4801 tagccgccgt ctggggtttg ccggacggag tgggcgcggt cgtcctcgct gaaggagtag
     4861 acccggcgcc acgtttcgtc cccggatgcg gtgatcttca tgacgagaaa gctgtagctg
     4921 tcggttacgt tcgtataggt atgcccggcc acgatgaagc cgccatcggc cgtgttcgag
     4981 atcgaggcgg cgcggtcgtt aaagctccag tcgaaggtcc tgcgccacag ttcgttcccc
     5041 tggtcatcta ttccaagcag gcataggtcg ctaaacccgg ttagaagatc gctcgtgaag
     5101 cccgcgatga caaagccgtt gtcgggggtc tttaccattg cccgcgcttc gctggaatcg
     5161 cccagggaat atattttgtt gtagatgtct acgccatcgg attttctcag ccagcggacc
     5221 agaatggccg agcgcgacgg gtccagaaga tcgatttgcg cgctcccggc gatgacatag
     5281 ctctcgccgg tctcctcgac ggcataggcg acgtcccagg attcgccgcc gtactccttg
     5341 tgccatgtgc cggggtcgcc gagattgccg ttcgtgtcga gtttgaggat gtaataattg
     5401 tcgagatcgc ggttgggggt gtccgattcc ctgcttccca caatgatata accgccgtcg
     5461 gaggtttgcc ggacggatcg cgcgacatcg cgctcggaga agccgtacag gcgctcccat
     5521 tccttttccc cgccacccga gagcttgagg acatagaaat cgttgatgtt gagcccgttt
     5581 tcgtactcgc cgacgacgat atacccgcca tcgagggtgc gttctatgtc gtaggcgaca
     5641 tcgtagccgg ggccgccgga ggcatagctc cattcctgaa cgccgaagag gtccagtttc
     5701 ttgacgagga cgtcggacac gccgctggat ccggtgccgg taaagccggc aaccacgcag
     5761 ccgtagtcag gggtggggcg gacggcgtat gcctgctcgt cgccgctgcc gccataggcg
     5821 ttcttccagg atatgacgct gacgttttcc gggagggtta cgccgccacc gccaacggtt
     5881 atcctgacga ggggattgtc gaaggtgtac ccggccttgg cgaaggcgag cgtatactcg
     5941 ccctcgcgca catcgctgaa ggtgtagacg ccgttcgcgt cggtactgac gtttcccacg
     6001 ccgccgtagg attcgtgagt gagcgcgacc gaggccgcct ggacggggcg gccgtacatg
     6061 gtgacgctgc ctgagattga attcgtgggg gaatcggcct tgcccatgca ggcgaccgag
     6121 agcgcgcaca gaaggcatgg aagcagggag gcgattgaaa ccgggacggc gcggcgcgtt
     6181 tttaccgcgc cggtcagtgc ccgatacgct tttccccgat gagcgccgac gggctcactt
     6241 ccgacgttta aacgcggaat acgaaacaat gaccacacga acttacccca atcacatcac
     6301 atgcgtattt ctttttacag catatacatc cgccacccgt ttgcggcggg tctgtttgcc
     6361 agtggtacgg tatctcttga acacaaacag gcagcgtgca cgccgggcgt gttttttgac
     6421 ccgatgtgcg tagttctctt tttatatttt ttaagccagc agtattaata ttttgttgac
     6481 ttaaatcaat tcagagatcg cgcctgtaaa aaatcaatcc aaattggacc cttgaaatac
     6541 atttttgaaa aaattgttgc tgacagcaaa tgtcatggtc gttttctcgt ttttgtctat
     6601 ttatgcgata ttccccgcct ggacgcctat cccgaatgcg cataacagga tttcttcccg
     6661 gattcctata tgtctagtgt ctccggaggg gacaaaatga caaaattttt cccgcgattt
     6721 acacattttc taaaaaggta accgggggca cggcgggtaa ggtgttgtgg aaatgcggct
     6781 ataataagga agagatttaa atgataacgc tagtacatga accgaagtcc ggccgttatc
     6841 atgaccgcct ggccgttttc gtccttttcg aagaagtaca cataccatgg ggccaggaag
     6901 aggcgcatcc ggccgcccag cgcatattgc agctcgccac ctgcgaatag agacggatcg
     6961 taggtgtagt gcatcttgta ttcatagcgg gtgcggccgt ccgggatgct tttgtcgccg
     7021 tagatgatgt gcccgagata gccgaacccc agcgagggaa cgacgtagag cccccgggcc
     7081 ggcgagagcg agtaccccgc cgtaacaagc gccatcgcga ggtaatagga ttgtatgctt
     7141 tcgaggcggt cgcgtatgcg atagccggcg gccccaagcg agacgaccac gcttcgccag
     7201 gggaccagcc gccagcgggc gtccgcggcg ataccgaagc catcgtgcgc tatgtttgaa
     7261 aacgcattga gcggcttcag atacccggca ctagcggcga ggctgtcgaa ctcgaggccc
     7321 cagcgcgctg cgggcgcgtc cggtccgccg gggcgcgcct gcgcggacgc atccatggcc
     7381 gctgacgcga cggcggccgc ccgaaggggc tccgccgcgg tcgcgcgtgc ccaggcgcgc
     7441 ttccggtagt actcgacgat ctttcgcgcg atctcccgcg ttttcgcctc gatctccgcg
     7501 cgggtgcgcg ggtattccgt aaaggacagc tcggttttcc cggtgggggt gtcgtgaacg
     7561 gcgacggtga gacgccagcg ctcctttttc gcgacccggt acacgtaccg ggagaccggc
     7621 tccgtgtcct cgatcgtatc gagctgcagc gtcccggtga cggttttatc cgagccgaga
     7681 acgcccccga tgcggacggc gcagtcccgg ccgcggcagg gaccgtgcgg ttcgcgccgc
     7741 tctttgagca gcgcggcgat ttccggttcg tttaccagcg tgaactcgcg ggacgccgcc
     7801 agctccgcat acagcgccgc ggaaagcccc cgcgcgaggg cgcgcgggct tcgcaccgcg
     7861 gccgcatcga acaccaggaa cgtgagccgg tcgtgcgagc cgcgcagcgg cgcgcgcgat
     7921 ggggcgcccc gagccgcgac ggcgagaagg cacgtcgcga gaacgatata tgctgttata
     7981 cgatacgcca tggtgcgatt gtcccgatcg ggccggggaa gcgatccggc ggcccgcatt
     8041 tttgtttttc gaggtgcatc atcgggacgc gcgtttgttt ttgtaaatga aaaaagcggc
     8101 gggggcggtt caaccgtatc gggcggtaga tgcactagaa gctacccttc gacatccaga
     8161 cggccctaac aatgcgcgat tttcaatttc gcggctaaaa atgaatcccg catgatgagc
     8221 ggttttcccc cgagcgtctc ggcctccgcg ccgcggcgcc gataccgaac tccggatatt
     8281 tcccgatgct catggatatt taaggcgcgt tactgcccca cacgtccaat gcccgagaag
     8341 gacccgcggg acgcttgcgg cgtgccgacc tgtgcgtaag tgcgtatcaa gtgagcggta
     8401 gcggatcata tcgacgatga ttctggaaac ctcaatatga ccgctcgttt accacgcacc
     8461 agaaatgggc aacgcatttg agcgcgttga gaaggtcttc gaattccatc ggtttcacca
     8521 cgtaggcatt gacgcccatg cggtacgcgt cccggatgtc gcgccgctcc cgcgaggagg
     8581 tgagcatgac cacgggtatg cctcccgtcc gctcatcgct tttcagccga ttcaggacct
     8641 ccaagcccga cattcccggc agtttgatgt caagcaggac gagggaaatg tctcgggcgg
     8701 aacagcccct gttggcgaca aactcaaggg ccccgtcgcc gtctcgtgac cgctcgacga
     8761 cgacggggac atcaatctcg ctcagggcca gggttatgag ctccgcgtcc cggtcgctgt
     8821 cctctaccag cagtatcgtt ttttcttgcc ccatgcgata ccccccgctg ttatacgccg
     8881 cgcgcgtccc ccggaaggga aaaaaacatg cacgctccct tcccgggttc gccttcggcc
     8941 cacaccatgc cgccatggcg ctggacgatg ctcttgaccg tcgccaggcc gatcccggtc
     9001 ccctcatact cgcgctcgtc gtgtagccgc tgaaagacgc cgaagagcct gtcgacatag
     9061 cgcatgtcga aaccggcgcc attgtccctg acataaaaaa catgagaact ttcagcggcg
     9121 cgatacccga tttcgatggc cggggacgac acccgcgcgg tgaacttcag ggcgttcgat
     9181 atcaggtttt ccatgacgac cctcagcagg gagggatcgc cgcgcacgac gggaagggag
     9241 gcgcgttcga tcgtaaccga tctctcggcg atctcctcct tgtgctcttt cagcagttca
     9301 tcgatcaggt ggctcatatt cacctctctt accgacagtc ccgttctttc catgcgcgaa
     9361 aacgagagta gatcgtcaat cagccgtccc atgcgcttcg cggaatcgtt tatcacatcc
     9421 atgtaccgtg aggctttggg tggaaaagag tccccgattt cccttcgaag catctccagg
     9481 aacccgttga tgtgacgcag gggggccctg aggtcgtggg agacggtgta cgagaacgac
     9541 tcgagttcct tgttggcctt ttcgaggtcg gcggtgcgtt cggcgacttt ccgttcgaga
     9601 tcggcataga gccgttccag ttctttctgg gcccgtttct tttccgagat gtccgtgacg
     9661 accacctgga cggcggggga gccccggtaa ttcagcgacg tcgccatgac ctcgacgtcg
     9721 atgaccctgc cgtcgagcct gacgtaccgg tcctcggcgg gatacagtcc ttcggcgccg
     9781 gagagcatgg cttctatcct ccggcgggcg ctctcgaggc cgtccgggtg cagtatcccg
     9841 gttatgggca taccgatcag ccgttcgggc gcgtccgctc cgagaatcga cgcgcccgcg
     9901 ggattgcaga agacgattat accgttttga tgaaccgcga taccgtgggg tattgtatcg
     9961 ataagcctgc ggtagcgagc ctcgctctcc cgcaggaggt ccccggcggc ttttctctct
    10021 gttatatcgt tcgcgagtac gagctcggct cttctattct taaacgtgag aacatgagaa
    10081 attatttcga caaagatgat ttccccgccc ttcgttcggt gacgccacac gccggcatag
    10141 tcaatgccga cgtcgacctt ttcaatgttt tcgatgaggc ggggaacatc ctcagacggg
    10201 cgaatgtcgg ctatggtcat tgaaagaaac tcgtcgcggg tatacccgta tttacggacg
    10261 gcggcgtcat tgactgccag gaagccgagg gtttcgaggt cgtacaccca catgggatgc
    10321 ggattggcct cgaagaggag gcggtaccgt tcctcgctct cccgctgggc caattcatgt
    10381 tccttgcgct cggttatatc ctgggctatg ccggacagct ctaccggaac gccggcctcg
    10441 tcgcggatta aatcacccgc ctccgcatat atccaacgga ccgagccgtc gggatgtact
    10501 acgcggtatt ccagcgggga cggcctgccg tcctcgatta ccgacctgtt cgatttttcc
    10561 accgcttcgc ggtcctcggg atggatcgcc cgggcgataa cgtccgccag ggcatcgttg
    10621 aaggaatcct tatcaatgcc gaagatgccg tacatcacct cagaccagat caggcggttt
    10681 tcggttatct tccaggtcca gtaaccgagg cgcgccaccc gctgggccct ttttaaaatg
    10741 gactctgatt cctgtaaggc gatctcgatt ttcttggcgt cggtaatgtc gcgcatgaca
    10801 tgcacgatgc cgttgggttc gcctgattca tcgaagatgg ggtacacggt gacctgcatc
    10861 catctgttcc gaaggaaaaa ttcgcccgat tcgggcttgc gggacacaga aactctcgcg
    10921 gcgggacaat cgggaatggg accgcccgta ccgtggacga tatcgaagca tttccttccc
    10981 gcgagatcgg acggcgatag cccaaaaaac gcgacgcttg ccgcattggc gcgaagtaca
    11041 cggaaatcct tatcgaggag ccataccgcg tcctggatgc tgtcgaaggt ggtgctccat
    11101 tcctgggcgg cgcgttcgat tttactctcg tattctttgc ggtctgttat gtccgtaccc
    11161 acggactgaa actccgcaag ccttccgtca tcgtcgaaga tcggtacgtc catccaggag
    11221 aaccagcgta tcgtgccgtc ggcgccataa accggatgct ccaatgtcgc aattcgagga
    11281 gtttccgata tttcccgata atactccatt atggcgctgc ggtgttcctc ggggacgaat
    11341 tcaatccatc gctttccctc tttctcgccg ggacgggcga tatgccggaa gtaatactcg
    11401 ttcgcgtagg tgagggtcgt atcgggacgc cagcggcaga ccgcgacagg ctggttttcc
    11461 acgatggctc ggtagcggcg ctcgctatcg ctgaattcca ccattttctt ttcgagcgtt
    11521 cgaggaaacg actcgtcgcg aaggtaattc aaatccgcca aggccgtgcc gtctttcggt
    11581 cttcgcgacg cggctcccgg aaattccccc aacgtttcct cgaataaact aaccagaaaa
    11641 tgcggttcca ccggtttcac gacgacccgc gcggccccca gagagagggc gaacgctttg
    11701 acgcgcgggt cagtatcggc cgttgcaata aaaacgaagg ggattttcga aaggatttcg
    11761 tcgttcatcc actcgcggca gagggtgaag ccgtccatga ccggcatgag gatgtccgtc
    11821 accgcgatgt ccggcggtac ggccctcgcc atggaaatgg cctcgcggcc gttcgccgct
    11881 ataagggcat gatacccacg ggccgccagg aaatcctcga ggcggatgcg gcgttcatcg
    11941 ataacgccca ctagcagtat cgtcttcatg cccgcactct ccgaatgact tgaccgcttt
    12001 ttacgtattc acatcatcac gccctgtccg cccttcattg cgacagccgg tacccgcggt
    12061 tcctgaaaag atgactgcag gcgtcgacga cggccgggtc gtacaatcgg ccccggttct
    12121 tcgcgatttc gtccagtgcc ggttcgagac caagggacgg ccggtatggc cggtgggacg
    12181 ccatcgcctc caccacgtcg gcgaccgcga ggatccgcgc ctccaagata atgtcttttc
    12241 ccgtaaggtt tcccgggtag ccggaaccgt cgagccgctc atgatggcga tacaccgcct
    12301 cggcgatggg ccagggaaac tcgatgtttt tcagtatctg gtaccccgcc tccgagtgct
    12361 ccttgatcat gctgaattcc acgggggaca gcttcgacgg ccgggtgagg atctccgcgg
    12421 gtacgtagat tttcccgata tcgtgaacga cgccggcaag gtagagccct tcgatggttt
    12481 catcatccag ccccatcaca gacccgattt cctttgaaag gtcagcaacg cgcttctgat
    12541 gacccgcggt atagggatcg cgatattcga gggccgtcgc catggcgtgg atgctttcga
    12601 acatgatttt ccgcatgcgc tcgaagctcg acgcaaggtc cgcggcggat ttaagacgct
    12661 gagatatatc gcgaaacgag atgacgcatc ccaggctgct gccgtcgtcc gtaacggtcc
    12721 tgacggtaac ctccgccggg aatgtcgtgc cgtccgcgcg ccgcagatta cggatgctcg
    12781 caccgccggg gacgggtgtg gggacgcacg ccaccgcaga cgcaccgccg ggaccgttcg
    12841 cttggtccgc gaagatcgtg tccagggact cgcccaaaag ctccttcatg ccgtatccca
    12901 ggatggcgag cgccgaccga ttgatgaata cgaaccgcag gtcgccgtcg agaccggcga
    12961 tgccttcatc ggtggagtcg aggatgatga tgttgcggtg acgaatgcgc tccagggcct
    13021 cttcggccct ctggcgctgg cggcgctcct cccgctcgtc aatggcccgc agtatggccg
    13081 acggaagctt ccccaggtgt tccttattga cataatcggt cgctccgcgc ttgagggctt
    13141 cgatggcacg ctcatcgccg atggtcccgc ttacaaaaat aaacggcaac aaccggtctt
    13201 gcgaccgtac ttcctcaagg gcctggaaac cgtcgtagga ggggagcctg aaatccgcca
    13261 gcacaacgct cggcttatac gtctcgagtg cgttcctgaa ggaagactcg tcggcgacaa
    13321 tccgtgtcgt gcaggggatc ccggcggcgg cgatttcatt ctcgatcaat ccggcgtcgt
    13381 tttcgctgtc ttcgagaatg aggacccgca ggagggcgtt cacatcggcc gcactcgacg
    13441 ataaggcgtt catatcggtt ccccctttac cgctacaccg ttcttgacgg aaccgcccaa
    13501 acgcatcaaa cggtccgctt gggccgccaa ggccatgaaa tcctcatcgt gcctcaattc
    13561 cggtttcttt tccgcaagcc tggcaagcgc gtctgaagcg gcccgcgcga tgccaccaac
    13621 ggcgttgtgc aggtcttccc atttctggcg gccgttcccg gctatgtccc ggatggactc
    13681 acgaaaatgc tttctccgta cggcctggtc tatggcgccg acgaggattt cgggggtgat
    13741 cagattctta ttaagataat tggcggctcc cattttcatg gcctgaacgg cagtttcctc
    13801 gttgccgtaa ccggttagca tgatcaccga aacgtccttt ccggcgtcat cggctttcaa
    13861 ctttcgaaac acggcaaggc cgtccatgtc cggtaggcgg taatcaagta aaacgcagtc
    13921 ggggtcgcag cgtcggtatt gctcaacggc ctccgacccg gttcctgcga agaagacatc
    13981 gtaccgtcgc gcgctttcgc gctcgagatg cgagacgata atctctctat cgtgagggct
    14041 atcgtcgacg ataaggacgg ttatccggct cggcggaacg tacttgggaa ggaagccgta
    14101 ctccggcaaa gcgtatcccg ctttctccat gagaacttcg aagggcattt gatacagccc
    14161 gctaattttc ctgatcgttt caggcgaggg tctgctgatc ttcccgtttt ctatttgact
    14221 caaataggcg ttcgacatgc cgcacagctc cgcagcctcc cgtatgctca aggccctttc
    14281 ttcacgggcc gctttaatga acgcgccaag ctctttcata taatcaccag acaccactat
    14341 cgcttgctgc atcaagcagt tcaatgatag tatagtcctt tttgttaact ccatcaagca
    14401 aatgtgtgcg tttttttaaa aattttcaaa aaattattgg tacttcggga agtggctact
    14461 taacggattc gttccggtaa tttccgctcc agttaaaccg ccggcggcca tacgtcacac
    14521 ccgcaggcgg ttcgaccatt cgactatctt tgaccgtgcc agcgaatatg aatcgaacat
    14581 gattcggagc tttttcccga gaagatcccg atcgctccgc tgcatgagcg caagggcggc
    14641 agcagtgtcg gcaaaggcgg cgtagacccg ttcacagttt ccgcatacga cctcgtgaag
    14701 ctcccgggcg agcccgaccg gtatgaattg ctgcggttcg cccatgacca gcgacacgcc
    14761 ctgattgctt gcgacggacg agatgaacag cgcggtgctg tagacgcgca caaaccgctc
    14821 gcagtcgggg agaacgtcga taagcgggcg aagaagcgca agctgggaaa tgtcgcgacg
    14881 gacctcacgc ttgacccagg tatagaccgt catgagcgaa ttcgagtgat aatccgaaac
    14941 gatcttcagg gaaacctcga aggagccggg gcgatgaaac agataccgat tttctttgtg
    15001 cgcggcgaga tcccgataat ctcgatattc ggggagatac agcgacggtt tggaaatgtt
    15061 gacatagcag agattgtcga gcaacgattt catccgcgtg gaaacccgga tcgcttcgag
    15121 ccgtttttcc tcctgttccg cgacctcgac aggcggggag ggtttcgttt tttcgcgctc
    15181 cacggccagg aggccgtcga gctggctgcc gatttcggcg atgctttcgg caaggaggcg
    15241 tttccccagg cccgcccgtt cttccagcgg agcgaggagt cccatttgtt cgagaacgcc
    15301 ggtcacgaaa tcacgcaaat cggaggcggc cacccgggtc ccattgattt tacggtcgat
    15361 ccacagaaac gttatggtat ggcgtataaa atcatcggta cgcgcggcgc cggcgccccc
    15421 tccggcgctc tttgcgatat tctggacgat cgtttttagc ttctcgcaat acaggatatg
    15481 attcacgtcg ctgagaaatt ccctgagccg cggttcgacg accgtgcgaa gctggtcctc
    15541 aaaacgttga agcgattttt cgagctcggt gacgtaggcg ttctttttcc gctccagcgg
    15601 tgtgcctgcg tgccgggcgt tgtggaggcg gtcttcccgc tcctggcggg cgatctcctc
    15661 cctgagcgcg gaggttttgt cgtctatgag gcgcgagtcg ataaggctgt tctggaagaa
    15721 caccagaatg tcctcgtacg cccggatatc gccgtttttg ccgttgtcga agagtttgat
    15781 gtgctcgtaa tagcggttta tctcggcaac gatcttgccg accctgtcac gaatttcata
    15841 gtcccccttc cgggagcctt tctcgtagct gagccctccg agctcgtaga acgaccgcgc
    15901 gaggatatac tttttctcgg acccgtcctt tattttgcgg gtcagataga gtatttcctt
    15961 cagttcgcta tttgcgccca tgggactacc gcaccgtcat cgtcatttgt ttattatcgc
    16021 acatctcgac tctcatctct ataccgcgtt agtttttcaa tgaccgtatc acgagtaaac
    16081 ggttattcca ggaccatcaa gaagcggcat ctcgacttca acgaactatt cgtccttgtc
    16141 cggatcgagc aattgattca aatcatcgaa gcccatgctt tcatcgtact ctccggggac
    16201 cattattcgc gatgtccctt ttttcgtgat aagcataccg cttacctcgc gattgaataa
    16261 tcgtacaaat ttcatgattt catcctcact gtcggtgaat ttcacgccga tttccctgcc
    16321 gtcgacgcgt atcactttcc cgggcatttg catggaacat ttacgaaacg taaaactgaa
    16381 caggacgttt tcaccgacgg ataccggaaa tgtctgcaaa aagcacagcc cgccagcgga
    16441 aatatcaacg atttcaattt tctcgaactc gccttttccg accgtttgaa ggcccgcaat
    16501 aaaccggatg acttttaatc ggggatacgt tcttcgctct tttgtcatgg gactgcagaa
    16561 cctcgctcag ggacggcgtt cattccgaat gtatcattaa accggattga cgtcaaatat
    16621 tgcgatgaag agcctttcat atatgactta gcccataaaa acggtaatag aactatatat
    16681 caactttctg cttgacacag caagcatatt ttagtatttt accatcgcga ggagccgcca
    16741 tgaaaaacct tgggaccttc atcaaggccg cgcgaaaacg cacggccatt accatccgcg
    16801 aggccgaaaa gcgcagcggg gtttcgaacg cctatttgaa ccagatcgag aacggacgga
    16861 tcaagcgacc gtcgccggac attctgggga agattggcgc attgtacgac ctgccctatg
    16921 atgtgctcct gcacaaggcg gggtattccc tgccggattt taagaccgat gaggacgccg
    16981 agcgcgaccg ctgctatcgg atactgatca tcgacgacga ccgtcgtgac cgggagctcc
    17041 tgataaacag gctcgccgga ttcaccggcg gcgccctggc ggttcgcgag gcccgttccg
    17101 gcgaggaggg gatcgcaatc ctcgaaaagg agctgtttga ctgcattttc ctcgactatc
    17161 gcatgccggg gatcgacggg cttgcggtgc tccggcggat ccgcgaggcg cggccctggg
    17221 aaacggcgtc gataatcatg ctgaccgggc aggggaacga gacagcggcc cttgaggcga
    17281 tccggctggg ggccgcgaat tatctgacga aaagcgagaa caaggagacc attctcgccg
    17341 cgctcaaaca ggccctgctg cgcaaacaga tgcggaggcc ggttgcggtc atgccgccgc
    17401 cccgcgacga cgaggaggcg ccgacagccc acgacgccct gacgcgcgtg cgccgcatca
    17461 ggaccatggt cgagaagata atcgaggccg acgatggcaa cgcgctgact gaggacctgc
    17521 gcggcatcct ccgggagata catgccgccg aaacggcact taccgcggag agccggcagt
    17581 cacgcgcgga gacctgacat gcgccgccga tgggaacgga caaccgagat acaacacacc
    17641 gcccgggtgg cggttgtttc tccgttcccc gcgaccatga ccgccgttca tgaggcgctc
    17701 gcggcgcacc aggacgacac cgtgtttttt gatcacgccg caacgccaca ggaggccatt
    17761 ggaaaaaacg ccgattggcg ctatgacgtt gtcctcatcg ccgcaggcga cggacccgac
    17821 gccgtcctta ccgcgctgac cgaatgcctc ccgcactttc cgagcgcccc cctgatcgcc
    17881 atcggcgaag ccgtcacgga atcgatggcc gccgccgcga tcctggcggg ggcggacgat
    17941 ttcgtttccc tgcgtgattt ttcgcggggg acgctctttc gcgccatcgc ccgcgccgcg
    18001 cagcacgcca accggaaact gctgcggggg acgagcgaat ggcgatttca cctgctgctc
    18061 gaaagccagg acgacctcat ctgcagctgg ctgcccgata cgacactcct ctatgcgaac
    18121 gactactatt gccgatttta cgggatcggc agggatagaa tcgggaccct gaaatggctt
    18181 gcgcttgttc ccgccgatat gcgcgagaac gtacgcgaga attatcaaac ctttctgaaa
    18241 gaaaaacgga ctatatcgta tgaacatgag gccaccgcgg ccgatggaag ggtcgggtgg
    18301 caggagtggc gcgacgtgcc tgtcgtggac gaacgtggcg agattgtcga gatcctgtcc
    18361 attggccgcg atataaccaa acggaagcag gcggagaatg cgctcaggga gagcgaggag
    18421 cgatttcgca ccctgtattc ctcaatggag cagggcgtgg tgtacgtaaa cgcctccggc
    18481 agcataacag acgcgaaccc ggcggccgcg agaatactcg gccgttcccg cgggcacttg
    18541 ctgggcatga cgctcgaaga cccgtgcata aaagccgtcg aagaagacgg ccgcgcattg
    18601 tccttcggct cgcatcccgc gatgatcgcg ctcaccaccg ggcgcccggc cctgggggtc
    18661 gtgttgggcg tcgaatcggc ccgcgaggga accgtccgct ggatcatgat agacgcggtc
    18721 ccgcagtttt tgccgggagc ggaaacgccc tgccgggcgt acgtgacgct gaccgacatc
    18781 accgcgctga aaaacgcgaa ggaggccatg cggcggttta acgaagaact ggagtcccgt
    18841 gtggccgaac ggacggagcg gctcgccgag gcgaaccggg agcttgaggc cttttcctac
    18901 acggtgtccc acgaccttcg cggtccgctg cggcatatcg ccggttttgc cgagatgctc
    18961 gctgagcatg ccggggggtc gctcgacgat acggggcggc actacctttc cgtcatcggc
    19021 gaatcggtcg gcaggatgga acagataatc agcggcatcc tgtcccttac ccgcatcggg
    19081 cagaccgagc tcaccatagc cgcggtggac ctggaccggc tcgtccgcga cattctgatc
    19141 tcgttcgagc cggaccttgc ggggcgggac atcgacatcg tcgtgtgcga cttgccgcgc
    19201 gtgcgcgccg acgccgtgct cctcgggatc gccctgcgaa acctcatcga gaacgcgatt
    19261 aaatttacgg cacacacgaa acacgcggta ctggaggtcg ggcatgcgcg cggtgcggga
    19321 acggacacgt tctttgtccg ggacaacggc gcggggttta acatggagta cgccgacaag
    19381 ctctttaacg cattcgagcg cctgcacgac acggacgagt acggcggtct ggggatcggg
    19441 cttccgacgg tcaagcgcat catcgaacgg cacggcggga ccgtttgggg cgaaagcggc
    19501 ccggacggcg gcgcgacctt ttatttcacg ctcggagcgc catcatcatg accgcgctcg
    19561 aaaaacgcat cctggtcgtg gaagacagcg cccatgacat cgaaatgatc tcgcgagcgt
    19621 tcgacctggc gggcattgtc gcatgtgtcg acacggtgcg ttcgggaccc gaggcgctgg
    19681 actacctcta cggcaggaat gcgtacgagg agacgggcgc ggggcgtccc gtgctggtgc
    19741 tcctggacat caaactcccc tttcgaagcg gcatcgaggt cgtgcgggaa ctcaaggaag
    19801 acgaaaatat gaaaacgatt ccggtgatca tgctcacctc atcggacgac gcgggcgaca
    19861 tcgaagcggc atatcgcgcg ggggcgaacg cgtatatcat aaagccgacc agtttttccg
    19921 agttcgtggc gatcatccgc agcatgacgc aattctggct gacggtgaac gaacatgcga
    19981 aatgaaacgg tgaaatctga tggtccggta tgccggcagg agcctttcat ggatggacgg
    20041 cgcgcaccgg ttcgggattt tcctgactat tattatgtct atatcatgac ggacccggac
    20101 cacgagctgc tgtttgtggg cgtttcgggt aacctcaagg cggtcatggc gatgtgccga
    20161 tttgagttcg agtatgacca tgggaccgtc agtatggaaa agcttgtcta ttacgagatt
    20221 tacgattccg ttgaaagcgc agtgatgcga aatcgcgacc tgcatctgct ttcccatgac
    20281 gccctgcggc acctcgtgaa cgccaccaac gtcgaatggg ccgacctctt tgaacagctc
    20341 gacggtatgg ccgcgacccc cggaggggta tgatgaagat catcgtagtg gacgacgaca
    20401 ccaaaacgac cgagctgctg ctggaatatg gcgggcttgg cgaagaggac gtatgcgcgt
    20461 ttcataaccc cgtagaggcc atcaagtacg tcgccaacca tgctattgat atcgccctga
    20521 tagactccca gacgcgggtc attgacagcc tgaatgttat caggtttata cggcgcatca
    20581 ggccatcctg cattgtatac gttttcgtga ccgaggcgat tgatatagac tacttttccg
    20641 tagcccacca cgtcggcgcc gccgatttca tccataaacc gctgcgcgag tcgtcctttt
    20701 cctttcttgc gggcgcggag gcatgacatg gcccgaatta cgaatggcat gattggcatt
    20761 gtcgttattg agcctgaccg gtggtgcatg ccgatgattg aacgcgcatg cgagatcatc
    20821 ggcgcccatg cgcggcgcat cgaggtgaac gaagcgacga gacgccccga cggctttgac
    20881 gcgctggtgc tctgttccgc ggccgccgat tccggactga tacgaaccat cgccggcgtt
    20941 tttcgcggtc cgctgctggt gatcaccgag accgccgatg cagtacgccg cccatcgttg
    21001 tatgccgacc gcacccgcct gatcgcgcga cccacgagaa gcattgacct ggcaaacgag
    21061 ctgctgcgcc tgctgaacgc gagcgcctcc gcaactgaca ccgcgcccca ccggtaacgg
    21121 tatatcatat gattccttcc atcctgtata ttggaacacg aaccgcggac gatgaactgc
    21181 tgcatcatgt gagcgaacga acgggacgcg caattctgcg cgttcgcgcc ctcggcgacc
    21241 tgcgcgcgcg aaacaccgaa tgcttcatcg atacggtgat cgtgggaacg acggttgagc
    21301 tcaccgcgcg ggtcctggac gagattgagc acctgcgcgg ccttgccgag gggaccgtca
    21361 tccttgggct ggtgcgcgag ggcatggacg atcgcgtgga catcatgata cagcgccgga
    21421 tgcagccctg ccatatctcc gcgaattcgg ccgcccgggc ggcgcgcatc gttgcgcaca
    21481 tgatagaaga acggaccggc gtataccgaa acgtcataga gcgcatgtgc aacgaactcg
    21541 cgcacggacg catggtccgc acgggcgggc tcgtggataa acggaagtga gggggcgtgt
    21601 tcctcgccag aggccctgta cctcctcgcg tggtcatcat gacatagcat aggcatcatg
    21661 gatgttattc aattttgcca ctgagaaaaa attttcatgg ccatggttgc tttcacacac
    21721 agggcgaatg aacgagaaga agcgtgtata ttcccgatag aatatagcgg agacacccac
    21781 aattgcgcgg ccgatatcgt tgtttttaag accaaatgac ggacagtccc ttgacgtcat
    21841 caaagcgcga atcgcttgag agaatgggga catcaagcca cagggcagta gcaataatca
    21901 tacgatcgtg aagttcgtaa aacttgatgc tttcggcttt ttttatgatt tcaggcgtaa
    21961 gatcgacaat ggaatacaat gatgaggcat ccagacgttc aagcgtcctg gacaggttta
    22021 tctttatacg attcttctct gaaagatata caatttccat aaggctgata acggagacat
    22081 gaaaggtgtg cggggtcgtt ggtccggcat cgagaacatc acgggcgcgg gcgccaatat
    22141 ttccgctccc gcacaaatga cggatgatgg ttacggtaac gagaagatac tgcatgaagc
    22201 gattaggcgt gttttttcag aatgacgctg gaaaggtccc tgcgaaggtc cctgatttcg
    22261 tcttcgagac tgggtagttt ctcgaacccg gtccccttcc agatgccctg aaggctcacc
    22321 gggcggggct ttgttccatc ggactgattg ataatggtgt cgatatatgt tttaacttcg
    22381 tcaagacggt tttcgggaac gaggtttatt ccttttttta ttattattcg ctttacgact
    22441 tgtgagtgca tacttcctcc atcgataggg gtaaactaat aaatggacat gagggagtca
    22501 acaaaaatcg cgcggcgccc gcggcggggt gccccaatga cgcattgatc gcgcacggac
    22561 gctttcgccc acagcgctcg ctcctgccac caccttcggc atacgactgt agtaaaacaa
    22621 caccgcgtga acggactacg gcattacggg acatgtattt tacctcacgg agaatggggt
    22681 gtggatcgtg gattgggtgc cgggggagtt tattgggttt ccggtttaac taacacttgt
    22741 gatatttttg tatattgtta ggtaatttat tgttgacgcg gattacctaa cacgatagaa
    22801 ttatattgtt gatgttaggt agtccccatg agcatactga aaggcgtact tgacgaagag
    22861 aacaagcgac ttaagcgtct gctgggcaaa tatcagcggg atattgacgc attgatcaag
    22921 ggatccgttt ccatcaagga tatatcgggg cagcggtatg cctatattgc caaacggaaa
    22981 ggggatacgg tcgtattcga atatatcggg aaagcgtcat ctgataaggt aaaaaggctc
    23041 ctggaagagg taaataaacg gaaaaagctg acggtgaaga tcaaggcact aaagcaggat
    23101 atcaaggaaa tcgagagggc gctcggtgga agaaagatat aggctttttg accagacgat
    23161 tgcggcactg cataacgccg gggtattatc ggcattggtg cttgtaggca gctggagcca
    23221 gcacctatac aagcacgccc ttacgaacac cgatgaaata ccattactca taacatcgga
    23281 cattgatttt ctgataccac aaccgaccca aatgacaatg ccatgcgatg tccccgggat
    23341 cctgaatgaa cttggatatg aaacggccac ctcgccggtc agtggacatg tcaagtatat
    23401 acgacgagaa ctcgaggttg aatttttggt ccctgaaaag ggaaggggat ctgacacccc
    23461 gcgaaaaata caggagctca tggtaagcgc acaggggctt cgctacctga acatgctgca
    23521 ggagcacacg ataaccgttg attacaaggg aatacccgta gccgtggtgg agcccgcggc
    23581 ctatgcgctg cacaagtttc tggtgagcgg gagacgcaca aagaaggaga agatggagaa
    23641 agacctcgct tcggcaatag ccatgtgcga gtacgtgaca cacgagatgt gcggtattac
    23701 aacgatgaga gatatcctga actctctgca cccgaagtgg aaaaaggata tactactaca
    23761 catcaagaag cacattcccg aattgcatga tagtctgatt tcacccggat agcgatgaca
    23821 gggatgcgtg ggaaaaacca tttctaaaat cgcgcggcgc ccgcggcggg gtgccccatt
    23881 gacgcattga tcgcgcacgg acactttcgc ccacagcgcg cgctcctgcc accaccttcg
    23941 gcaaacgagc gcatacctca cccacacgcg aacggactac ggccacacgg cccaccggca
    24001 cagccggcag agcgcaaaca gcgcggcgcc gacggcgggg tgccccattg acgcttggat
    24061 cgcgcactga cgctcgcgcc cacagcgcac gtcggtgcca tcaccttcgg cacacgagcg
    24121 cattaaaaca tacccgcgca cacggactac ggcattacgg cacaaacggc aacagccaga
    24181 ccgacgacgg cggtacggcc cgggcggcgt tcacggcaac ggccccacac ggccaaagac
    24241 cccttaggat gaacgaaaac actaagtcta aaaaaacaca cactcacgca taaaggcggc
    24301 tgtgttcctg tatgtaccgg tccgtggtgt acagtcgctc gttgaagtac cagcggctga
    24361 cgggttgcgg attacagaac agattgagtt gtgtcgattt tctcggctcg cgcgccggca
    24421 cgaaccagca cccttccttt gccccgatac gaacgacggt ccccgttggt tgagccctgt
    24481 cccctttgac cccgctcaac acgcatgacg tgtacgtatt gggtgcatta agcctcgcca
    24541 cccattcccc gcccttgtag tggcggggga ggggggaggt gatgccgtga acgtttaaga
    24601 gtatggcgcg aaacttctgg acctcctcgt aggagcccgt gaagtcctcg agaaaattgt
    24661 aacgcttgcg aaaggtgccc tctttgtgat tacgcgcggt ggcgtactgg atccgccgca
    24721 cggtgcagcc ggaatagcgg gcgatgtttc cgttcgcccg gaacttgttg ccggcgagaa
    24781 ggacgccgac gcagaaatcg tagaactccc gccgcgacgc gaggacgctt tcgtacggca
    24841 gctcgaacca catggccggg ccggaggaat tttgacggtt taacagggac ttctctgagc
    24901 ggatgcggta gcgaccgtcc gagagcggca cgaacaggac cgaggagccc aggaggcgcg
    24961 ccagacggcg gcggacggcc ccgcggttgt tgcccgggcg gcggtgaagc gaggcgtagc
    25021 cgtactccgc gtggagtatg tcgcacagct ccgccaggct aaagatgcct gaacgcgcgg
    25081 cgaagtgccg gcgggcgatg acgtagtagc gatagtcatc gaacctgccg ccggtgcccc
    25141 ggacgagctt gatgatgccc ctgctcaggc gcagcattga tatagtgccg tggtgacgga
    25201 ctagctcaga tgccgcgcgg ttatgcgcgc gattttacga tgagtggaaa aatcgcgatg
    25261 agatgggaaa aaaatcataa aaaaacaccc cttttgatcg attttttgat ccagcgggtg
    25321 ttttcgaatt gacaatactc gaactgcgtt agtatattca tcctaacaat aaccaattgt
    25381 cgattcgggg taacacccga aattaagaag cccggagagc cacctccggc ttctttgttt
    25441 tttggaccag cgttacatct tccgctatgt cattgatgaa ccttccctga acagataccc
    25501 gataaacggg agtacaaccc gcgacgggac gtttgtgaac gcggggacag gctagcagcg
    25561 gccgaaaaat taatcaataa cttttttatg tcacattaaa aggctccgac aggcccccgc
    25621 atgcccgaaa atgggggcat gcgagacgcg aaatgaagac ggaaagcgca ttcgcacgaa
    25681 atgcgagcga atggacgatg aaatagattt ttgcattgcg tcgttttcca aaaatgaaac
    25741 aactctctat aagattaatg gcccgagcgg gggttgcggg acagattttt ttaaaatttt
    25801 cattcgtccg cgcgtatgag gacgtacgcg cggacgcgga agagagacag cgggcggtga
    25861 aaccgaagat tgcaggcgtg cagacggcgc agccgggacg ccgggcgggc attcagagcg
    25921 cgtcgaccag ctcgaggatg ccacagcgcc tgagacggcc gaagacaaag cggacggccg
    25981 tggcgttgag atgcctgacc cgacggcgcg agagccccag gcggtgcccc acgtccgcat
    26041 gggtgcggcc ctcgaggg
//
LOCUS       DAORHF010000028        25612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1206_length_25612_cov_6.635403, whole genome shotgun sequence.
ACCESSION   DAORHF010000028 DAORHF010000000
VERSION     DAORHF010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25612
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1206_length_25612_cov_6.635403"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /gene="asnS"
                     /locus_tag="PKJ16_04180"
     CDS             <1..1007
                     /gene="asnS"
                     /locus_tag="PKJ16_04180"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010997802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HNV46217.1"
                     /translation="NTFGAVLRVRNALARAIHEFFQDRGFVYVHTPIITASDCEGAGE
                     MFAVTTLKLEDIPRTKDCVDYAGDFFGRKTGLTVSGQLEGELLATALGDIYTFGPTFR
                     AENSNTSRHLAEFWMVEPEMAFADITDDMDLAEDFLRHIFAACLERCADDMAFFDERV
                     QRGAIETLRGIADSRFERITYTDAIDMLEKSGESFEYPVKWGLDLQSEHERYITEKKI
                     KKPVIVIDYPREIKAFYMKLNDDGRTVRAMDVLVPRIGEIIGGSEREDRYDVLLDRMR
                     AAGLSAEDYWWYLDTRRFGSVPHAGFGLGFERTVQFVTGMQNIRDVIPFPRTPGSAAF
                     "
     gene            complement(1068..1763)
                     /locus_tag="PKJ16_04185"
     CDS             complement(1068..1763)
                     /locus_tag="PKJ16_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46218.1"
                     /translation="MIARRCFMLFIAATLLAGAAGCSQTFNMNLKPTAIQAKSAPAVL
                     PVFVKMVNPPVDSFVFAVTPIGAKKKMEMKNIPGRFDELLKQQIEQALKNRGYAIASS
                     PGAARGVLTVDFHTFDFLWRVSAVSSSFGQTNILIKNSLAANRANAQPAWKWQYQNDA
                     PYGSMWGNCYQNGCAFISGITIIGAIPFLIYLKARREPWDYLSEAGNIRMDEFLKQLE
                     TQLPRAEAMRGRL"
     gene            complement(2027..2479)
                     /locus_tag="PKJ16_04190"
     CDS             complement(2027..2479)
                     /locus_tag="PKJ16_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46219.1"
                     /translation="MRAFGPVDLALLVWELPQNLLGALVLWRLKRRGAVVEIVNERRR
                     LFIKTITAVSLGYFIFWYRDDGNPVFDITAVNRDHEYGHSVQSRMLGPLYLPVIGLPS
                     YLRTVYARWYYRRYGAKWKGYYRGYPERWADRIAGVDSHAGTRSRPHE"
     gene            complement(2493..2930)
                     /locus_tag="PKJ16_04195"
     CDS             complement(2493..2930)
                     /locus_tag="PKJ16_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46220.1"
                     /translation="MKYIGAVIAACALACTLAQAEEFIKNGWANANTYRVTGTGTIGR
                     DQDGGTPAQRQVLACEAAQLHAEGQLVERFAQARLRSVKGSVSEEAVRDGIKRRFAGT
                     IRGGRIVWRSFDPATNECRVVYEISEKNLKARVTGQTRAKKKK"
     gene            3080..3418
                     /locus_tag="PKJ16_04200"
     CDS             3080..3418
                     /locus_tag="PKJ16_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012220512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HNV46221.1"
                     /translation="MTASTTRPARYWIYILECRNGSYYTGYTSDMDRRWEEHCAGSPK
                     SKYTRGFAPTRIVARWRIRGTRGTAMRIERLIKSLTRVHKEELVARPETLGALIRDRL
                     GVRMRPKHEG"
     gene            complement(3454..3951)
                     /locus_tag="PKJ16_04205"
     CDS             complement(3454..3951)
                     /locus_tag="PKJ16_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46222.1"
                     /translation="MRNHRTPLAALLAVIMIAVLAVAAAAATGNVRFVNVSVDKNGLV
                     MKELKEEQYWVDTGGIKRIVELHFSDPQLFSRRPAGLSLILNIPDIEEADSEDGLEVF
                     LNDRPVGSVPRVARNTWLEIPLDVSAFDSAPEFFLVIKSKTSDSIAIASKKSGFGAVI
                     KAVYR"
     gene            complement(4047..4649)
                     /locus_tag="PKJ16_04210"
     CDS             complement(4047..4649)
                     /locus_tag="PKJ16_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HNV46223.1"
                     /translation="MLPALAIALSLLCAAPDFSRLAEGDIIFQETSGRQSDAIALATK
                     SRYTHVGIVFREKGRWMVLEAVQPVSSIALERWIARGVKRHYVVKRLAAAPGGIGPDA
                     VQRMKAAAVKYLGRDYDEFFEWSDRRIYCSELVWKLYRETLGVELAPRAKLGDFDLSA
                     PAVRRELRRRYGGSVPVNEPVVPPSALFDSKLLVTVMAAP"
     gene            complement(4652..6100)
                     /locus_tag="PKJ16_04215"
     CDS             complement(4652..6100)
                     /locus_tag="PKJ16_04215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV46224.1"
                     /translation="MLLRVRACAIVPALCLGILGMGEWTCKKKEEPRYAGGVNPSGYR
                     MLEGKKVFVDAGHGGSLGADIFRQGPGGVSEEEVNLRVARYLRDKLAAAGAIVRMSRE
                     KDEDVPLERRVAMAKEFAPDLLVSVHHNGTVRRMDRVNYPCVLFWGSAHNAGPSLNLA
                     ELLQNEFHKIMEDRGRVLSDFAAFPETGTMILRETRYLCPGVIGEAGFFSDERHALRL
                     ADLQYNQLEADAYFLAIAEFFRRGAPSGTVRASCAVESDPIAGLLIKDDRPVIALRLD
                     PGAKDATIDRSSVRAWVDGVPAGVTLIEDALCVLDYGERLYPGAHAVRFSFANSRGNR
                     SPVMHATFTLEIKAGDFDRLMGEGVRLVKSRRNPREGLKMLLAARSMSVTHPDDDTIL
                     WHIAQGFARIGDANASDYYLAKIRHCYPESSYLPKSARTQSEPRMMVEYKGAPVSFLH
                     EASLEHVCREHTRKKTPSAPVRRSGPRLNEVI"
     gene            complement(6124..9096)
                     /locus_tag="PKJ16_04220"
     CDS             complement(6124..9096)
                     /locus_tag="PKJ16_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46225.1"
                     /translation="MRVVPAARDGFDMWLFNYNAISNFLICVLLVIMVGYLLFRSQRN
                     REVLSLILFMAHLLVWQACLLYQNLYFDPAAALPLFYFQHSGYTLLSFLALVMFSYVF
                     IEPVFATEARVVFFAGLLAGFVIIIHFFVSVLPVPPQMRFDVALHVYSASPAGIRPYI
                     IALELMFLAIYAKNMVYKSIRMRGESRLFAIHVAVATLAGAVLIMGGVVLEGAGAVDP
                     RFMQGANTVLAGIVFIYFYWVYINFSRMRYFYADKTFLTIIFIVIIAISLTTALTYGI
                     YRDSYVADRREDARNIVTALDSRAISDAGAYFEELHKADLDFISVRDNATGAERSLFG
                     TLYHFEGRGLDARGAGSERPAPVFASYGATVLFYFTLQGGARTVWTGFSYVAYRAHMH
                     RFVAAGLTTIVLVVFGLFALLRVAIYFGLTRPLRRLLRGIEEIHAGNLYHEIDVNTFD
                     EIGYVSQQFNYMVYDLRVTEESIKRSEQKYREIMAMLPDIIYETDLSLRITYLNEAGR
                     RITGYAEDDVHAGVSMTELLDESDTGALRALLAAAPARRSVEFITHRIRRKDGSVFYG
                     ENNSVVIDEMGRPAGLRGVIRDVTERLRLERSLLQAQKMKTIGSLAGGIAHDFNNILA
                     GITGTVSLLEYKLAGSAALRPEEIGDDLRIMKTTADRAAKMVRQILALSRMQTPTLAL
                     TDLNAVLRHVLDVCKNSFDKKIELDFTFHEGAAYATADETQLEQVVLNLCVNARDAMT
                     IMRPAGSAAGGRIVVRLEHSFPDEDPFSPARADADAAYWCISVADAGVGMDNETLARV
                     FDPFFTTKEKDKGTGLGLPMVYNIVKQHGGFVDISSEPEKGTVCRVYLPIARPEPEAS
                     REERAPEGLHFGSGTILLVDDDGAIQKTAEEMLRALGYGVILARDGREGVEVLRARAD
                     EIDAVILDVVMPVMSGHEAFAQMRALVPDLRVLVSSGFRDDPRIEEILSAGARGFIQK
                     PYTLRQLSVELHRIVQME"
     gene            complement(9235..9474)
                     /locus_tag="PKJ16_04225"
     CDS             complement(9235..9474)
                     /locus_tag="PKJ16_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46226.1"
                     /translation="MLDGAQRKTWFGITLFLLVATGLIPIGGGTGPYVARMPIFMFYL
                     LLAMGEVKALWFILPHVCIAGALGYWIARYAGRSA"
     gene            complement(9545..10183)
                     /locus_tag="PKJ16_04230"
     CDS             complement(9545..10183)
                     /locus_tag="PKJ16_04230"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV46227.1"
                     /translation="MIGDIHGMAGKLSGLYRRISRELADDDTLVFVGDYVDRGPDSFR
                     VIELLRELSARHDAVFLKGNHEELFLDALEGRNREAFLENGGDATVASYRAALGAMRL
                     PEPHEAFYGGLRLYYEADDFVAVHAGLKPNSYNVETQDPADLLWIREAFYRHPRRWEK
                     TVIFGHTPSMTLNGRTAVYRDARRNIIGIDTGAAYGGPLSCVRMPDGKSFQY"
     gene            complement(10200..11273)
                     /gene="mnmA"
                     /locus_tag="PKJ16_04235"
     CDS             complement(10200..11273)
                     /gene="mnmA"
                     /locus_tag="PKJ16_04235"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013651883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HNV46228.1"
                     /translation="MRIAVAMSGGVDSSCAALLLREQGHDVVGITAKLLLCADMDGVE
                     PRDDVCCSPESIADARGVARRYGFPHVVADVEDDFSREVIDPFCREYVRGRTPSPCIT
                     CNARIKFPRLLAIARELGCAALATGHYARIGEESGRYYVSRGVDPDKDQSYFLFDLSQ
                     EVLAQVRFPLGGYRKEEIRALARGYGLSVADKPESQEICFVLDNDYPRYIERRTGTIP
                     PPGDIVDRAGSVLGRHRGVHRYTIGQRRGLGIAHPTPLYVIEIDASRNVIVVGGRDEL
                     ARRGLVATGINRMKAVTLGGCDAFVKTRSTQPPVRARLEDAAGGVSAVFAEPQAGISP
                     GQAAVFYDEQGDVLGGGWIERAL"
     gene            11466..12440
                     /locus_tag="PKJ16_04240"
     CDS             11466..12440
                     /locus_tag="PKJ16_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV46229.1"
                     /translation="MPHPNDAFTPPIALRNPIIQSVLASSALRAIGPNPMLAAEREVI
                     VDAGGGVRLQGFLSTHSARPRSLVILLHGWEGSAASAYMKRTGRFLYRTGYDVFRLNL
                     RDHGTSHHLNEGLFLGTLIEEVFAAIRAISAGAGVPSVLAGFSLGANFALRVARRAAV
                     EPVPGLRHILCVNPPLDPHASTLAIDGIGIFRNYFLKKWKRSLAIKQSCFPAAYDFSG
                     ILAMRSCMAITDALMARHTSFASSREYFDGYTLTDGRVDRVGVPTTVITAEDDPVVPA
                     RDFHALEGVKDLSLIVHRYGGHCGYIENVLLRSWYQNALAEIVARVAR"
     gene            complement(12450..12911)
                     /locus_tag="PKJ16_04245"
     CDS             complement(12450..12911)
                     /locus_tag="PKJ16_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46230.1"
                     /translation="MAVDTTMYVHKSTLGLVKVVCTRAGLKRNRAIVLLLKGAMTDRA
                     LKAAIGRAIRYQGRDRKDNWRTVHVRLREDEADYFKDLRNVLKLSVSLILAIAARRYL
                     AELVRKAKQGEKMDNYPFSHYVLSREEQEDGMIFWKICWGFPEHPEHHFRL"
     gene            13065..13859
                     /locus_tag="PKJ16_04250"
     CDS             13065..13859
                     /locus_tag="PKJ16_04250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV46231.1"
                     /translation="MYIDTHAHFDICIEEGVATEQTLIDELAASDIGRAVQISIDEDG
                     FAWSRDFARRRRDRGVLFTAGIHPSSQSDDAKLGRLSAFLDDSLAGDGADLLLGVGET
                     GLDFYRMRQPRDAQERSFEFQLGAAKRRGLPVIVHIRDAMDEGIAIMTRFAPLTGIMH
                     CFSGDSAAARRVIDLGLLVSFAGNLTYPKATQLHDAARYVPLDRLLLETDAPFLTPVP
                     YRGKPNRPAFVTHTYAFFATMRKEPLANVIDTIEQNFDTLIAGRRG"
     gene            13978..15666
                     /gene="nadB"
                     /locus_tag="PKJ16_04255"
     CDS             13978..15666
                     /gene="nadB"
                     /locus_tag="PKJ16_04255"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HNV46232.1"
                     /translation="MNPRRHHSDFLVIGSGIAGLAFAIKAAALGSVNIVTKKKDSDSN
                     TNYAQGGIASVLSPEDSIDEHIADTLKAGAGLCNAAAVEILVRNGPERIAELMEWGTR
                     FSTRIDREGTRALDLGREGGHSHNRIAHAKDLTGQEIERALINKISTMRNIAVFEDHT
                     AVDLLTEHQLRLGSAGESSGPAVDPITCYGAYILENETGTVHIFTARCTLLASGGAGQ
                     VYLHTSNPDIATGDGIAMAYRAGAFIADMEFIQFHPTTLHQDRLEGRSFLISEAVRGE
                     GALLFNSRGERFMEGEHELKELAPRDIVARAIDRELKRLGDRCVYLDITAKGEDFLRH
                     RFPLIYENCLKAGYDMARERIPVVPAAHYLCGGIVSDTFGRTAIRNLFVSGESACTGV
                     HGANRLASNSLLEGVVFSHRAFLHLADTWHGRVDAPTPEFPHWNKEGTFDLEEWILIQ
                     HNVEDVKRLMWDYVGIVRSDLRLQRAYRRIILIKEEILDYYRRSTISPRLIELRNLAT
                     IAKLIIQSALARKESRGLHYNTSYPFGSEQQRKNVVLRAGQPPSMTAIDTLPVA"
     gene            15753..16025
                     /locus_tag="PKJ16_04260"
     CDS             15753..16025
                     /locus_tag="PKJ16_04260"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HNV46233.1"
                     /translation="MPKELILSGRVQGVFCRDYCRQNARRLGLRGSASNLANGSVRVL
                     LDCDDERVDEYVRALRGNPFGFRFHGRISDITVTEYRGGMGGDYVF"
     gene            16049..16924
                     /locus_tag="PKJ16_04265"
     CDS             16049..16924
                     /locus_tag="PKJ16_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46234.1"
                     /translation="MRIRALGIIILTTVALASCISIDFVKTGKTYTALKPNADVIVFH
                     RSAPTERYEEIGILRIDGGSLSKRIEVAKKVARQNGGNGLVPRGSGSDALRSVDSKIF
                     EHESKVADAKGSERTQVFMVIRITDPEAERRAKEAEAKGPEGSPDKDSSKFTQRDPDK
                     YYREGTDGPNEPGRSPERDPGKLPRATYRQLIENIDTMKGQRFAGALVPEKFYKIPRT
                     LRSSAGPDSKLLMMRTTQRRQQVLVIVPADKAAGFKESIGSRSRVTIVYTPVDVHRFG
                     GKAYPVLKLVDDAKK"
     gene            complement(16985..19681)
                     /locus_tag="PKJ16_04270"
     CDS             complement(16985..19681)
                     /locus_tag="PKJ16_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HNV46235.1"
                     /translation="MAVTTTSLPGYGSISTLEADAALRLLGSSPDGLTGARAAALLAE
                     HGHNELPPAKRKGILRKVFDALADPMALILLVAALFSLVIGDVIEAVAIMFVVVVNTV
                     IGIAQDRKAEKAVEALTKMLAPQSRVVRNGSTELVAARFIVPGDIVIIEAGDIVPADA
                     RIIESHGLLVDEAFLTGESDPVRKTAGALPGTDRKLYEMENMVFAGSRVLDGSGRALV
                     AGTGTRTEMGRIAGSMQALEEEKTPLQRKLQTEIKFLVGLAFVSAALVVAITLLRVTG
                     IDNLNRETLFGVVLVAVSVMVAVFPEGLPASITIALSLAIQRLAKNSIIIKGLTSVET
                     LGNVDFICTDKTGTITAHVMTVKEYYIGDGFLTGGDIFRMIAEGETGVIRDIFLASFK
                     TSTAQIVETDGTVEKELGDPTEVALLKAAVIAGFRPAQFESAQVVDRVPFSSDLMYAA
                     SLVAEPAGERAILVKGAPERIVALCDRHYVDGAEEKLGKHDAARILDDLAGRSEKGYR
                     LIAFARKGAASGTDTIDRARLGGFVFLGAAVIFDPPKDEVKLTVCEAKEAHISMVMIT
                     GDSKKTGRSIAESVGIADGASQAVEGRELEAMTEAEFTRQVENIHVYSRVAPLDKLRI
                     VDKLKEKGHVVAMTGDGVNDAPALKRADVGIAMGRAGSQVAQEAADVILTDDDFATIV
                     NGIREGRIVYQNLRRLVRYLITNNLGKVAGLLLNPILGFPTPLLAIQLLWTNVIMEAF
                     PAVGISTDAAGPGIMKKPPVRLADPIFALRDRVHMIVDGLVFGCAISAGYILVDHLTN
                     DRAIAGTASFTITLLSPQIYVFMLRDGSLRQKLFSFNMILHPFLAFTVAMIAAIVYVP
                     HLNIIFKTAPVRDPALWLVIGGLSVATSVFRLGVGTFFKERG"
     gene            complement(19742..19912)
                     /locus_tag="PKJ16_04275"
     CDS             complement(19742..19912)
                     /locus_tag="PKJ16_04275"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HNV46236.1"
                     /translation="MPPKKTQVPQLDEFLSEIRQHAYEVFEERIRRNEPGDEMSDWLR
                     AEREVKAKYGIA"
     gene            complement(19993..20604)
                     /locus_tag="PKJ16_04280"
     CDS             complement(19993..20604)
                     /locus_tag="PKJ16_04280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF039181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HNV46237.1"
                     /translation="MAGAPAPARLRTIDLTGELESIIRDICASVPMFAHIDMGRVLVC
                     VASNRGIGGGGCYGKLVPLRFAGGAEIARFRGRSFAIPRVVAGERRMLYVIYFYFPRF
                     FDLDARGKLRVIFHELYHISPDFNGDIRRMGPVRAAHGHSRRRFDRRFEAECDGYFDR
                     IRSTGVAEMLSLDGDGLHGRFERVLGRRLKVPRPVEVGSREDL"
     gene            complement(20609..22345)
                     /gene="recN"
                     /locus_tag="PKJ16_04285"
     CDS             complement(20609..22345)
                     /gene="recN"
                     /locus_tag="PKJ16_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HNV46238.1"
                     /translation="MTAAGRERHRMLRELRIKNFVLVDELRLPFGPGLNILTGETGAG
                     KSVLIDALSGVLGEKMTTDMIRTGFDRAVLEGEFDVSGLPDVRRLLDEAGIDCDDGVL
                     MIRRELNSNGKGRCFANATQIPVARMAEIAAYLVDIHGQNEHQNIVRIAKHRELLDAF
                     AGHGALVDAVRALHARLGEIKEKMDTFEIDEREKARRIEYLTFAVKEIEQAKLSPCEE
                     DELRAESTVLANAERLFREISTATALLKGDGGIVGGLKKVEQSLAAIAGFDANITDTL
                     ESVKQSLYAMEDVAAGLREYERSVNFSPERVNEVEERLALISSLKKKYGSTVDEILEF
                     RERSLRELEAFSSSEEEIERLRVEYREAVREAKETALDLSERRKNTAKRLEELVMKEL
                     RDLGMAGTVFRVSIKREIHPDGIIEADNKTYMLYPHGLDRVEFLLSANEGEDLRALAR
                     VASGGELSRIMLALKNVILASDIVESLVFDEVDAGIGGKTAEVVGKKLKTLSRDRQVL
                     VITHLPQIAAMSDSHFIVKKEKTANRFTTIVKNITRSEKVREVARMLAGETITELSLR
                     HAEEMVSRAEGA"
     gene            complement(22342..23205)
                     /locus_tag="PKJ16_04290"
     CDS             complement(22342..23205)
                     /locus_tag="PKJ16_04290"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HNV46239.1"
                     /translation="MIATVSANIRPDDDGSLAFLGSMIDFLRKRGVATLLPPYDIFSA
                     TPFAGCVTDMARFVRDAELVVAIGGDGTFLRTARLFADTGKALFGVNRGRVGFLTEFM
                     PDEALPHLAAAVEGRGEIAERSLLEVEHWRNGAMMERQVFVNDAVISKGAFSRPIDIE
                     LAIDGDFLNRFSGDGLIVATTTGSTAYSLSAGGPIVAPPVDSVYVINPICPHVLAVRP
                     MIVSSACTLRARIESRFENLLLTIDGQVAIRIEAGDDVYFRGTDTTIRLVTHPDFSYY
                     DILRQKLGWGR"
     gene            complement(23207..23656)
                     /locus_tag="PKJ16_04295"
     CDS             complement(23207..23656)
                     /locus_tag="PKJ16_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HNV46240.1"
                     /translation="MFKRVLYPTDFSDCAGAAVPYLRKLKGAGTAEIVVVHVIDLRHA
                     AFAETVSWFGESIKQYEMELFNNLTQDAQRRIDEVCRRLGRDFTVRGIVESGVPFKTI
                     IDVARREKATLIVLGSHGASNIAEMLMGSVSEKVVRKSPVPCLVVKR"
     gene            complement(23669..24712)
                     /locus_tag="PKJ16_04300"
     CDS             complement(23669..24712)
                     /locus_tag="PKJ16_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46241.1"
                     /translation="MNHRIFLVGVAFAAALGCSPEHESKMLKGIEAYRQGAIEEGTLR
                     AAEGLVLSSKIAKFKKGEPVASGNVIYTRSDNTVRLHHPCAASIDVPAQFKHLACDPA
                     TGRIAATNGLELRIFGKGGASESSVVVSQDKKDKADSLAVSDDAVFYFVGRQLHRYSI
                     ADRKIAVVAGRDKIQPPFIGVPYYSHLYKSGTTMMVILGIAGDYTANIVDLAADSLFM
                     KDIKVNSPRMWFENNRLWYVSGIIGMWQLVELSVPDKKKREVFKFRDIADVAFFPGGM
                     LYEDREGLWAVLTGPRHLVKLPAQLKIAGMAHGAMLLTLEHATYLVDSTRLAAAIAFL
                     AEKGPGMCAVKKF"
     gene            complement(24720..>25612)
                     /locus_tag="PKJ16_04305"
     CDS             complement(24720..>25612)
                     /locus_tag="PKJ16_04305"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46242.1"
                     /translation="SRAITADPSLVDCVLPIGVASYYLSEYDDAEAFLLRAVEHPQSM
                     FEANFYLGVIYKGKKDFDTAIRMFENSRKDRAFFVKSLYHLGEIYFENGLYDKAIEAL
                     EEGIGRIDKKDDEALAYRYLLAECFEIENKIKEAVHHWEQIARINSDFRSTRLKLEEY
                     QNILNNPNLKSFFTSSITELQPLIQEVISRLNYNIVSKKEVSHNEYVYKAFNIKRINE
                     PPVLIVFNRTTKEITEGQIINFHRTIGAEKCKSGMYITTSKFSIRARSSASSRMVELY
                     DSTFLDKAVEKIKAKTAKNE"
BASE COUNT         4603 a         8564 c         7994 g         4451 t
ORIGIN      
        1 ccaacacctt cggggcggtg ctgcgcgtgc gcaacgccct ggcgcgcgcc atccacgaat
       61 ttttccagga tcgcggcttc gtgtacgtcc acacgcccat tatcaccgca agcgactgcg
      121 aaggcgcggg cgagatgttc gccgtcacca cgctgaagct ggaggatatc cctcggacga
      181 aggactgcgt cgattacgcc ggcgacttct tcggccgaaa gaccggcctc accgtgagcg
      241 gccagctcga gggcgagctc ctggcgacgg cgctcggcga catctacacc ttcggcccca
      301 ccttccgcgc ggagaactcc aacacctcgc ggcacctggc ggagttctgg atggtggagc
      361 ccgaaatggc cttcgccgac atcaccgacg acatggacct ggccgaggat ttcctgaggc
      421 acatcttcgc cgcctgcctg gagcgctgcg ccgatgacat ggccttcttc gacgagcgcg
      481 tacagagggg cgcgatcgaa acgctgcgcg gcatcgcgga ttcgcgcttc gagcgtatca
      541 cctacaccga cgccatcgac atgctggaga agtccgggga gtcgttcgag taccccgtga
      601 aatggggatt ggaccttcag tccgagcacg agcgctacat caccgagaaa aaaataaaaa
      661 aaccggtgat cgtcatcgac tacccgaggg agatcaaggc cttttacatg aagctcaacg
      721 acgacggcag gacggtgcgg gcgatggacg tgctggtgcc tcgcatcggc gagatcatcg
      781 gcgggagcga gcgggaggac cgctatgacg tgcttctcga ccgcatgcgc gcggcgggcc
      841 tttccgccga ggactactgg tggtacctgg acacccgccg cttcggcagc gtgccgcacg
      901 ccggcttcgg cctgggcttc gagcgcacgg tgcagttcgt caccggcatg cagaacatcc
      961 gcgacgtgat cccgtttccc cgcacgccgg ggagcgcggc gttttagcgc atcacgacgg
     1021 agcgaacccg gtcacggcgc gcacgaccgg gaccgcccgc gatcacctca taatcgtccg
     1081 cgcattgcct ccgccctcgg cagctgcgtc tcaagttgct tgagaaattc gtccatgcgt
     1141 atattgcccg cctcgctcag atagtcccac ggctccctgc gcgccttgag gtatatcaaa
     1201 aacggaatgg cccctatgat tgttatccct gaaataaacg cacatccgtt ctgatagcag
     1261 ttgccccaca tactgccgta cggcgcatcg ttctgatatt gccacttcca cgcgggctgc
     1321 gcgttcgccc ggttcgccgc gagggaattc ttgatgagga tattcgtctg gccgaaggag
     1381 ctggacacgg cggacaccct ccagagaaaa tcgaatgtgt ggaaatcgac cgtgagcacc
     1441 ccgcgcgcgg cccccggaga ggatgcgatg gcatatccgc ggttcttcag cgcctgctcg
     1501 atctgttgct tgagcaactc atcgaaccgg ccgggaatgt tcttcatctc catcttcttc
     1561 ttcgccccga tcggcgtcac cgcgaagacg aatgaatcga ccggcggatt gaccatcttg
     1621 acgaacaccg gaaggaccgc cggcgcggat ttcgcctgga tcgcggtggg cttgaggttc
     1681 atgttgaacg tctgcgagca ccccgcagct ccggcgagga gcgttgcggc aatgaagagc
     1741 atgaaacaac gtctggcgat catatcaacc tcctgtatcg aataacgttt aatcgggacg
     1801 atcggtcgca tacacgcccc gaactccccg gatgacgccg gttcacacgg aaatcgatca
     1861 aaaagcccgt tcgggcgaat gggttgaatt gttctgtcta tctatctttc gtctgcatat
     1921 ttggcgccac agaccttttt aataaatata acaaacgacg tcgacctgtc aagttttttt
     1981 acgagcggcg tgccgacggg atcaccatcg ctgaacgctc cgccacttat tcgtgcggtc
     2041 gggatcgcgt cccggcgtgc gaatcgacgc ccgcaatccg gtccgcccag cgctcggggt
     2101 atccgcgata atagcccttc cacttcgcgc cgtacctgcg ataataccac cgcgcataca
     2161 ccgtacgcag atacgaggga aggccgatca cgggcagata gagcgggccc agcatgcggg
     2221 actgcacgct gtgaccgtac tcgtgatcgc gattgacggc cgtgatgtcg aatacgggat
     2281 tcccgtcgtc acgataccag aagatgaagt atccgaggga caccgcggtg atggttttaa
     2341 taaacagtcg gcggcgttcg ttgacaatct cgacgacggc gcccctccgt ttcaaccgcc
     2401 agaggacaag cgcgcccagc aggttctgcg gaagctccca caccaacagc gcgagatcga
     2461 cgggaccaaa ggctcgcatt aaatgcgtat atctactttt tctttttcgc gcgcgtctgt
     2521 ccggtcacgc gcgccttgag gttcttctcg ctgatctcgt agaccacccg gcattcgttc
     2581 gtcgcgggat cgaacgagcg ccatacgatt cgcccgccgc gaatggtgcc ggcgaagcgc
     2641 ctcttgatcc cgtcccggac ggcctcctcg ctcaccgagc ccttcacgct ccgcagcctc
     2701 gcctgcgcga agcgctcgac gagctggccc tcggcatgga gctgcgcagc ctcgcaggcg
     2761 agcacctgac gctgggccgg ggtgccgcca tcctggtccc ggccgatggt gcccgtgccc
     2821 gtcacccgat aggtgttcgc attcgcccat ccgtttttaa tgaactcctc cgcctgggcg
     2881 agcgtacacg ccagcgcaca cgcggcaatc acggcaccaa tgtatttcat gccacctcca
     2941 ctacggaacg acgcgatatc cgccatcgaa tggaatcatg cgtttcgccg tccgtcaaat
     3001 cgttttttcc ttgcgtcccg tgcgcgattc gcataccggt attgacgaag cgccatcgac
     3061 agtgggaaaa aaatttgcca tgaccgcctc gaccacgcgc ccggcgcgct actggattta
     3121 tatcctggaa tgccgaaacg gcagctacta tacgggatac accagcgaca tggaccgccg
     3181 ctgggaggag cattgcgcgg ggagcccgaa atccaagtac acgcgcggct tcgcgcccac
     3241 gcgcatcgtc gcgcggtggc gcatacgcgg cacgcgggga acggcgatgc gcatcgaacg
     3301 gctcatcaag tcgctcacgc gagtgcacaa ggaggagctg gttgcccggc ccgaaacgct
     3361 cggcgctctg atcagggacc ggctgggggt gcggatgcgt ccgaagcacg aaggatgatc
     3421 cgcgccgggg gcgcacccgg ttgagcgacg ggactaccgg tacaccgcct tgatcaccgc
     3481 gccgaagccc gacttcttcg atgcgatggc gatgctgtcg ctcgtcttgg acttgatcac
     3541 cagaaaaaac tccggcgcgg aatcgaaggc gctcacgtcg agcggtatct cgagccaggt
     3601 gttgcgcgcg acccgcggca cggagcccac cggccggtcg ttcagaaaca cctccaggcc
     3661 gtcctcggaa tcggcctcct cgatgtcggg gatgttcagg atgagcgaaa gccccgccgg
     3721 gcgtcgggag aagagctggg ggtccgagaa atggagctcg acgatacgct tgatcccgcc
     3781 ggtgtccacc cagtactgct cctccttgag ctccttcatg accaatccgt tcttgtccac
     3841 cgagacgttc acgaatcgga cattgccggt cgcggccgcc gcggcgacgg cgaggacggc
     3901 gatcatgatg acggccagca gagcggcgag gggcgtgcga tggttgcgca tggcggtatc
     3961 tccacaagga tcatgccggg attgctgttg ttatcatcgg catggcgcgg agaaaaatga
     4021 agcggactct gtcgtccgat gcgggcctac ggcgccgcca tcaccgtgac caggagcttc
     4081 gaatcgaaca gcgcgctcgg cggcaccacc ggctcgttca ccgggaccga tccgccgtag
     4141 cggcgccgca gctccctgcg caccgcgggg gccgagagat cgaagtcgcc cagcttcgcg
     4201 cgcggcgcga gctccacgcc cagcgtctcg cggtagagct tccatacgag ctcggagcaa
     4261 tagatgcgcc ggtcggacca ctcgaagaac tcgtcgtagt ccctgccgag gtatttgacc
     4321 gccgcggcct tcatccgttg tacggcatcc gggccgatcc cgccgggggc ggcggcgagg
     4381 cgcttcacga catagtgccg cttcacgccg cgggcgatcc agcgctcgag ggcgatcgac
     4441 gagaccggct gcaccgcctc gagcaccatc cagcgcccct tctcgcgaaa gacgatcccc
     4501 acgtgggtgt agcgcgattt cgtcgccagc gcgatggcgt cgctctgccg gccgctggtt
     4561 tcctgaaaga tgatgtcgcc ctcggcgagc cgcgagaaat ccggcgcggc gcacagcagc
     4621 gagagcgcga tcgcgagcgc gggaagcatc gctagatgac ctcgttcagg cgcggcccgc
     4681 tccggcgaac gggcgcggac ggcgttttct tgcgcgtgtg ctcgcggcag acatgctcga
     4741 gcgaggcctc gtgcagaaag gaaaccggcg cgcccttgta ttccaccatc atgcggggct
     4801 ccgattgcgt gcgcgccgat ttcggcaggt aggagctctc gggatagcag tggcggatct
     4861 tcgcgaggta gtagtctgac gcgttcgcgt cgccgatccg cgcgaagccc tgcgcaatgt
     4921 gccacagaat ggtgtcgtcg tcgggatgcg tgacgctcat cgagcgcgcg gcaagcagca
     4981 tcttcaggcc ctcgcgcgga ttgcgccggc tcttcaccag ccggaccccc tcccccatca
     5041 gccggtcgaa atcgcccgct ttgatctcca gcgtgaacgt cgcgtgcatg accgggctgc
     5101 gattaccgcg cgagttcgca aacgagaacc gcacggcgtg ggccccggga tagaggcgct
     5161 cgccgtaatc gagcacgcac agggcgtctt cgattaaggt gacgccggcg ggaacgccgt
     5221 ccacccacgc gcgcaccgac gagcgatcga tcgtcgcatc cttcgcgccc gggtccagcc
     5281 gcagtgcgat gacgggccgg tcgtccttga tgagcaggcc cgcgatcggg tcgctctcca
     5341 cggcgcacga ggcacgcacc gtccccgacg gcgcgccgcg gcggaaaaac tcggcgatgg
     5401 ccagaaagta cgcgtcggcc tcgagctgat tgtactgcag gtcggcgagg cgcagggcgt
     5461 gccgctcgtc gctgaaaaac ccggcctcgc cgatcacgcc ggggcacagg tagcgcgtct
     5521 cgcgcaggat catcgtgccc gtctcgggga aggccgcgaa gtcggacagc acgcggccgc
     5581 ggtcctccat gatcttgtga aactcgttct gcagcagctc ggcgagattc aggctgggcc
     5641 cggcgttatg cgcgctcccc cagaagagca cgcacgggta gttcacccgg tccatgcggc
     5701 gcaccgtgcc gttgtggtgc acgctcacca ggaggtccgg cgcgaactcc ttcgccatcg
     5761 ccacgcgccg ctcgagcggg acgtcctcgt ccttctcccg gctcatgcgc acgatcgcgc
     5821 cggccgccgc gagcttgtcg cggaggtagc gcgccacgcg caggttcacc tcctcctcgc
     5881 tcacgccgcc cggcccctgc cgaaagatat cggccccgag cgagccgccg tgacccgcat
     5941 ccacgaacac ctttttcccc tcgagcatgc gatagccgga cgggttcacc ccgcccgcgt
     6001 agcgcggctc ctccttcttc ttacaggtcc actcgcccat gccaaggatc cccaggcaga
     6061 gcgccggaac aatcgcgcac gcgcgcacac gcaggagcat tcggttcacg gttcatcacc
     6121 tcgtcattcc atttgtacaa tacggtgcag ctccaccgaa agctggcgga gcgtatacgg
     6181 cttctggata aaaccccgcg cgcccgcgga aaggatctcc tcgatccgcg gatcgtcgcg
     6241 aaaaccggac gagaccagca cccgcaggtc cgggaccagc gcgcgcatct gggcgaacgc
     6301 ctcgtggccc gacatcacgg gcatcaccac gtcgaggatc accgcgtcga tctcgtcggc
     6361 gcgcgcgcgc aggacctcga ccccctcccg cccgtcgcgg gcgaggatca ccccgtaccc
     6421 gagcgcccgc agcatctcct cggcggtctt ctggatggcg ccatcgtcgt ccaccagcag
     6481 tatggtgccg ctgccgaaat gcaggccctc cggcgcgcgc tcttcgcgcg acgcctccgg
     6541 ctcgggacgg gcgatgggaa gatacacgcg gcacaccgtc cccttctcag gctccgagct
     6601 gatatcgaca aacccgccgt gctgcttgac gatattgtac accatgggca ggcccagccc
     6661 ggtccccttg tccttctcct tggtggtgaa gaacgggtcg aacacgcgcg cgagcgtctc
     6721 gttgtccatg cccacgccgg cgtccgcgac gctgatgcac cagtacgccg cgtccgcatc
     6781 ggcgcgcgcg ggcgagaacg ggtcctcgtc gggaaagctg tgctccagcc ggacgacgat
     6841 gcgtcctccc gccgcactgc ccgccgggcg catgatggtc atcgcgtcgc gcgcattcac
     6901 gcagagattg agcaccacct gttcgagctg cgtctcgtcg gccgtggcgt aggccgcgcc
     6961 ctcatgaaag gtgaaatcga gctcgatctt tttgtcaaag ctgtttttgc acacgtccag
     7021 cacatgccgg agcaccgcat tgaggtccgt gagcgccagc gtgggcgttt gcatgcgcga
     7081 tagggccagg atctgacgca ccatcttcgc cgcgcggtcg gcggtggtct tcatgatgcg
     7141 caggtcatcg ccgatctctt cgggccggag cgcggccgac ccggcgagtt tgtattcgag
     7201 cagggacacc gtcccggtga tgccggcgag gatattgttg aagtcgtgcg cgatgccgcc
     7261 ggccaggctc ccgatcgtct tcatcttctg cgcctgcagc aggctgcgct ccagcctgag
     7321 ccgctcggtc acgtcgcgta taacgccgcg cagcccggcc ggccgcccca tctcgtcgat
     7381 caccacggag ttgttctcgc cgtagaacac gctcccgtcc ttccggcgga tgcggtgcgt
     7441 gatgaattcc accgaacggc gcgccggcgc cgccgcgagc agcgcgcgca atgcgccggt
     7501 gtcgctttcg tcgaggagct cggtcatgga aacgcccgca tgcacgtcgt cctccgcgta
     7561 gccggtgatg cggcgcccgg cctcgttgag gtaggtgatc cggagcgaca ggtccgtttc
     7621 gtagatgatg tccggcagca tcgccatgat ctcgcggtat ttttgctccg agcgcttgat
     7681 gctctcctcg gtcacgcgca ggtcgtagac catgtagttg aactgctggg agacatagcc
     7741 gatctcgtcg aaggtattca cgtcgatctc gtggtacaga ttgcccgcgt ggatctcctc
     7801 gatgccccgc agaagccggc ggagcggccg cgtgaggccg aaatatatcg cgacgcgcag
     7861 gagcgcgaag agcccgaaga ccacgagaac gatcgtcgtg agcccggcgg ccacgaaccg
     7921 atgcatgtgc gcgcgatagg cgacatagga aaagccggtc cacaccgtcc gcgcgccgcc
     7981 ctgcagggtg aaatagaaga gcaccgtggc gccgtaggat gcgaacaccg gcgccggccg
     8041 ttcgctaccc gcgccccgcg catcgagccc cctcccctcg aaatgataca gcgtgccgaa
     8101 caggctgcgc tccgcccccg tcgcgttgtc gcgcacggag atgaagtcca ggtccgcctt
     8161 gtgcagctct tcgaaatagg cgcccgcatc ggagatggcg cgcgaatcca acgccgttac
     8221 gatgttccgc gcgtcctccc ggcggtcggc cacgtaagag tcacggtaga tgccatacgt
     8281 gagcgccgtc gtgaggctga tggcgatgat gacgatgaaa atgatcgtga ggaaggtctt
     8341 gtcggcatag aagtaccgca tgcgggaaaa gttgatatac acccagtaga agtagatgaa
     8401 gacgatcccc gcaagcaccg tgttcgcgcc ctgcatgaag cgcggatcga ccgcgcccgc
     8461 gccctccaga acgacgcccc ccatgatgag aaccgctccg gcgagggtcg cgacggccac
     8521 gtgtatcgcg aacaggcgcg actcgccgcg catgcggatc gacttgtaca ccatgttctt
     8581 cgcgtagatg gcgagaaaca tgagctcgag cgcgatgatg tatggcctga tcccggccgg
     8641 cgacgccgaa tagacgtgga gcgcgacgtc gaaccgcatc tggggcggga cgggaaggac
     8701 gctcacgaaa aaatgtatga tgatgacgaa cccggccagc aaaccggcga agaacaccac
     8761 gcgggcctcg gtggcgaata ccggctcgat gaacacgtac gagaacatca ccagggcgag
     8821 aaacgacagg agcgtgtatc cggaatgctg gaaataaaaa agcggcaggg ccgcggcggg
     8881 atcgaaatag agattctgat agagcaggca ggcctgccac acgagcaggt gcgccatgaa
     8941 caggatcagg ctcagcacct cgcgattgcg ctggctcctg aacagcaaat agccgaccat
     9001 gatgacaagg agcacgcaga tgaggaaatt gctgatcgcg ttatagttga acaaccacat
     9061 gtcgaagccg tctcgcgcgg cgggaacgac ccgcattcgc cggagtatgt ctgtctagtg
     9121 gcgcatcgat gcgccgcggt caataccgta gcgtgccggt ccgcacgtgt caaacataat
     9181 tcaattcccg atccgcgcga ttccgattcg gcgaaccccc cggacgaacc tcgctcaggc
     9241 ggatcggccc gcataccgcg cgatccaata gccgagcgcg cccgcgatgc acacgtgcgg
     9301 cagaatgaac cacagcgcct tcacctcgcc catcgccagc aggagataga acatgaatat
     9361 gggcatcctt gccacatagg gtccggtccc gccgccgatg ggtataagcc cggtcgcaac
     9421 aagcagaaag agcgtgatcc cgaaccacgt tttgcgttgc gctccatcca gcatccgatc
     9481 gcctcccgcg ttccatcgtc gattcgtgcg cgcgcgacac atcatgcccg ccgcgctccg
     9541 cgcctcagta ctgaaacgat ttcccgtccg gcatccgcac gcacgaaagc gggccgccgt
     9601 acgccgcgcc ggtgtcgatg ccgatgatgt tgcgccgcgc atcgcggtac acggccgttc
     9661 gtccgttgag cgtcatgctg ggcgtgtggc cgaagatgac ggtcttctcc cagcgccgcg
     9721 ggtgccggta aaacgcctcg cggatccaca gcaggtcggc cgggtcctgc gtttcgacgt
     9781 tgtacgaatt tggcttgagg cccgcgtgca ccgcgacgaa atcatcggcc tcgtaataca
     9841 ggcgcaaccc gccgtaaaac gcctcgtgcg gttccggaag ccgcatcgcg cccagggccg
     9901 cgcgatagct cgccaccgtc gcatcgcccc cgttctcgag gaaggcctcg cggttgcgtc
     9961 cctccagcgc gtccagaaag agctcctcgt gattcccctt gagaaagacc gcatcgtgcc
    10021 gcgcggaaag ctcccgcagg agctcgatca cccgaaagga gtcgggcccg cggtccacgt
    10081 agtcgcccac gaacaccagc gtatcgtcgt cggcgagctc gcgcgagatg cgccggtaca
    10141 ggcccgaaag ctttcccgcc atgccgtgga tgtcgccgat cacgtagtgc atccgccgcc
    10201 tacagcgccc gctcgatcca tccgccgccg agcacgtcgc cctgctcgtc gtagaacacc
    10261 gcggcctgtc ccgggctgat cccggcctgc ggctcggcga agacggccga cacgccgccc
    10321 gcggcatcct ccagacgcgc gcgcaccggc ggctgggtcg agcgcgtttt cacgaaggcg
    10381 tcgcacccgc caagcgtcac ggccttcatg cggttgatcc ccgtcgcgac gaggccgcgc
    10441 cgggcgagct cgtcgcggcc gcccacgacg atcacgttgc gcgacgcatc gatctcgatc
    10501 acgtacagcg gcgtcgggtg cgcgatcccc aggccgcgcc gctggccgat ggtgtagcga
    10561 tggacgccgc gatgccggcc cagcaccgac cccgcgcgat cgacgatatc gcccggcggc
    10621 gggatcgtcc ccgtgcggcg ctcgatgtag cgcgggtagt cgttgtccag cacgaagcag
    10681 atttcctggc tctcgggctt gtcggccacg gagagaccgt agccgcgcgc gagggcgcgg
    10741 atctcctcct tgcgatagcc gccgagcggg aagcgcacct gcgccaggac ctcctgcgac
    10801 aggtcgaaga gaaaatacga ctggtccttg tccggatcga cgccgcggga cacatagtac
    10861 cgtcccgact cttcgccaat ccgcgcatag tgcccggtgg cgagcgccgc gcagccaagc
    10921 tcccgcgcaa tggccaggag ccgcgggaac ttgatgcgcg cattgcaggt gatgcacggg
    10981 ctgggcgtgc gcccgcgcac gtactcgcgg cagaagggat cgatcacctc gcgcgagaaa
    11041 tcgtcctcca cgtccgcgac cacgtgcggg aagccgtagc gccgcgcgac cccccgcgca
    11101 tcggcgatgc tctccggcga gcagcacaca tcgtcgcgcg gctcgacgcc gtccatgtcg
    11161 gcgcacagca ggagcttcgc cgtgatgccc acgacatcgt gcccctgctc gcgcaggaga
    11221 agcgcggcgc acgacgaatc cacgccgccg ctcatggcga ccgctatgcg catttccgcc
    11281 cgcccccggg caatagaatc gtgccgtcgc agtccatcat catcacccca aaaatcgttc
    11341 gcgatagcat acggctgcgc cggcggctgg caagaattat tttacccgca aatttccatt
    11401 gacagcgcac tcgtgggcga tgatgatgcg cgccggtatt cgtattttcg aacggagcac
    11461 ctgacatgcc gcacccgaat gacgccttta ccccgccgat tgcgctgcgc aatcccatca
    11521 tccagtcggt gctggccagc agcgcgttgc gcgcgattgg accgaacccg atgctggcgg
    11581 cggagcgcga ggtgatcgtc gatgccgggg gcggcgtgcg gctccagggg tttctctcga
    11641 cgcactcggc gcggccgcgg agcctagtga tcctgcttca cgggtgggag gggagcgccg
    11701 catcggcgta catgaaacgg acggggcgct ttttgtatcg cacgggatac gacgtcttcc
    11761 gcctcaacct gcgcgaccac ggaacgagcc accatctgaa cgagggcctg ttcctgggga
    11821 cgctcatcga ggaggtgttc gcggcgattc gcgccatcag cgccggggcg ggcgttccca
    11881 gcgtcctggc gggattttcg ctcggcgcca atttcgcgct gcgcgtcgcg cgtcgtgcgg
    11941 ccgtcgagcc cgtgccggga cttcgccaca tcctgtgcgt gaacccgccg ctcgatccgc
    12001 acgcctcgac gctcgcaatc gacggcatcg gcatcttccg caactacttt ctcaagaagt
    12061 ggaaacgctc gctggcgatc aagcaatcct gcttcccggc cgcctatgat ttttccggca
    12121 ttctcgcgat gcgctcctgc atggcgatca ccgacgccct gatggcgcgg catacgagct
    12181 tcgcgtcttc gcgcgaatac ttcgacggct acaccctcac ggacggccgc gtggaccgcg
    12241 tgggcgttcc gaccaccgtt atcaccgccg aggacgaccc ggtggtcccg gcacgggact
    12301 tccatgcgct ggagggcgtg aaggaccttt ccctcatcgt ccaccgctac ggcgggcact
    12361 gcggatacat cgagaatgtg ctgctgcgtt cgtggtatca gaacgccctg gcggagatcg
    12421 ttgcgcgggt ggcgcggtag cgcacatcgc taaaggcgga aatggtgttc ggggtgttcg
    12481 gggaaccccc agcagatttt ccagaatatc atcccatcct cttgctcctc tcgagaaagg
    12541 acgtaatgtg aaaatggata gttatccatt ttctcccctt gcttcgcctt tctcaccagc
    12601 tcggccaggt atcgccgagc ggcaattgcc aggatcaacg aaaccgacaa cttcaacaca
    12661 ttccgcaagt ccttgaagta atccgcctca tcctcccgca accgcacatg gactgtacgc
    12721 cagttatctt tcctgtcgcg tccctggtag cggatcgccc tgccgatggc cgccttcaat
    12781 gcgcgatccg tcatcgcgcc cttaagaagc agcacgatcg cgcgattgcg tttcaacccg
    12841 gccctcgtgc atactacctt gaccagcccc agcgtcgatt tatgcacgta catcgtcgta
    12901 tcaactgcca tatccccctc catggctcct ctacactggt gatacggctc gcgcaccatc
    12961 gcgtgtaaca ttttccgttc tcgcccgata gaaaaaattt gtttgacacc gctgcatccg
    13021 atggcaatca acaaggcgac acgaattttc acggacggcc gaccatgtac atcgacacgc
    13081 acgcgcattt cgacatttgc atcgaggagg gcgtcgcaac ggaacagacg ctcatcgacg
    13141 agcttgccgc gagcgacatc ggacgcgccg tccaaatctc aatcgacgag gacggtttcg
    13201 cctggtcgcg tgatttcgcg cggcggcgcc gcgaccgggg cgtcctcttc acggccggga
    13261 tccacccgtc ctctcaatcg gacgatgcga agctggggcg gctctcggcc ttcctggacg
    13321 attcgctcgc gggcgacggg gccgatcttc tcctgggcgt gggcgagacc ggcctcgact
    13381 tctatcggat gcgccagccg cgcgacgcgc aggaacgctc gttcgagttt cagctcggtg
    13441 ccgcgaagcg ccgcggcctt ccggtcattg tccacatccg cgacgcgatg gacgagggga
    13501 tcgccatcat gacgcgcttc gcgccgctta ccgggatcat gcattgcttt tcgggtgata
    13561 gcgccgccgc gcggcgcgtg atcgacctgg gactcctggt cagcttcgcg gggaacctca
    13621 cctatccgaa ggcgacgcag cttcacgatg cggccagata cgtcccgctc gaccggctgc
    13681 tgctcgaaac ggacgctccc tttctcacgc ccgtaccgta tcgcgggaag ccgaaccgac
    13741 ccgcgttcgt gacgcacacc tatgcgtttt tcgccacgat gcgaaaggaa ccgctcgcga
    13801 atgtgattga caccatcgag caaaacttcg acactttgat cgcggggcga cggggctgat
    13861 atcgcccgcg gccgaacggc cagccgcacc gaaggagaac cctatgcacg cacgccgctg
    13921 gatcgacacg cccgttcgct tcgctgtgat gatgcgcacg gctcggagca tggcgcgatg
    13981 aacccgcggc gccatcactc ggacttcctg gtgatcggct ccggcatcgc cggactcgcc
    14041 tttgccatca aggccgccgc gctgggcagc gtcaatatcg tcacgaagaa gaaggactcc
    14101 gactccaata cgaactatgc gcaagggggg atcgcctcgg tcctgtcgcc cgaggactcg
    14161 atcgacgagc atatcgccga caccctgaag gccggcgcgg gcctgtgcaa tgccgcggcc
    14221 gtggagatcc tggtgcgaaa cggccccgag cgcatcgccg agctcatgga atgggggacg
    14281 cgcttctcca cccgcatcga ccgcgaggga acgcgcgccc tcgacctggg acgcgagggc
    14341 ggccactcgc ataaccgcat cgcgcacgcc aaggacctca ccggccagga gatcgagcgg
    14401 gcgctcatca acaagatatc gaccatgcgc aacatcgccg tcttcgaaga ccacacggcg
    14461 gtggacctcc tcaccgaaca ccagctgcgc ctgggttccg ccggcgaatc atccgggccg
    14521 gcggtcgacc ccatcacctg ttacggcgcc tatattctcg aaaacgagac cgggaccgtg
    14581 cacatcttca ccgcgcgctg cacgctgctg gcctccggcg gcgccggcca ggtgtacctg
    14641 cacacctcga atcccgacat cgcgacgggc gacggcatcg cgatggccta tcgcgctggc
    14701 gccttcatcg ccgacatgga gttcatacag ttccacccga ccacgctcca tcaggaccgg
    14761 ctcgaggggc ggtcgtttct catcagcgaa gcggtccggg gagagggcgc cctcctgttc
    14821 aactcgcggg gagagcgctt catggagggc gagcacgagc tcaaggagct tgcgccgcgg
    14881 gacatcgtcg cgcgcgccat cgaccgcgag ctcaagcgcc tgggagaccg gtgcgtctat
    14941 ctcgatatca ccgcgaaggg agaggacttt ctccgccacc gctttcccct catctacgaa
    15001 aattgcctga aggccgggta cgatatggcg cgagagcgca ttcccgtggt gcccgccgcg
    15061 cattacctgt gcggcgggat cgtgagcgac accttcgggc gcaccgcgat ccgcaacctg
    15121 ttcgtctcgg gcgaatcggc ctgcaccggc gtccacggcg cgaaccgcct ggccagcaac
    15181 tccctcctcg agggcgtggt cttctcgcac cgcgccttcc tccaccttgc ggacacctgg
    15241 cacgggcgcg tcgacgcccc gacgccggaa ttcccgcact ggaacaagga gggcaccttc
    15301 gacctcgagg agtggatcct catccagcac aacgtggagg acgtgaaacg cctcatgtgg
    15361 gactacgtgg ggattgtgcg ctcggacctg cgcctccaac gggcctaccg gcgcatcatc
    15421 ctcatcaagg aggagatcct cgactattac cggcgcagca ccatctcgcc gcgcctcatc
    15481 gagctcagga acctcgcgac catcgccaag ctcatcattc agagcgcgct cgcgcgaaag
    15541 gaaagccgcg gacttcacta caataccagc tatccgttcg gctccgagca gcagcgcaaa
    15601 aacgtggttt tgcgcgccgg gcaaccgccg tcgatgaccg cgatcgacac cctccccgtc
    15661 gcctgatccg cgtaaaaaag tacttgaaaa tacccgaccc gccgggtatc cttatttcgg
    15721 ttcgccattg acagcgacgg gacagaccgg ggatgccgaa ggaactcata ctgagcggac
    15781 gggtgcaggg ggtcttctgc cgagattatt gccggcagaa cgcccggcgg ctgggcctgc
    15841 gcggctcggc cagcaatctc gcaaacggct cggtgcgcgt ccttctcgat tgcgacgacg
    15901 agcgcgtcga cgagtacgta cgcgcgctca ggggcaaccc tttcggcttc cgcttccacg
    15961 ggcgcatcag cgatatcact gtcaccgaat accggggcgg catgggcggc gattacgtct
    16021 tctgagtcgg accgcacagg aggaaaacat gagaatcagg gcacttggca tcatcatcct
    16081 cacgactgtc gcgctggcgt cgtgcatcag catcgatttc gtgaaaaccg gcaagaccta
    16141 cacggcgctc aagcccaacg cggacgtgat cgtgttccac cggtcggcgc cgacggagcg
    16201 ctacgaggag atcggcattc tgcgcatcga cggcgggtcc ctgtcgaagc gcatcgaggt
    16261 ggccaaaaag gtggcgcgcc aaaacggcgg caacgggctg gtgccgcgcg gatccggctc
    16321 cgacgcgctg cggtcggtcg actcgaagat cttcgagcac gaatcgaagg tcgccgacgc
    16381 caagggttcc gagcgcaccc aggtgttcat ggtgatccgc atcaccgacc ccgaggccga
    16441 gcgccgcgcc aaagaggcgg aggccaaggg cccggagggc tccccggaca aggactcgtc
    16501 gaaattcacg caacgcgacc ccgacaagta ctatcgcgag gggacggacg gtcccaacga
    16561 accgggacgg tcgccggagc gcgatcccgg aaagctcccg cgcgcgacct atcgccagct
    16621 catcgagaac atcgacacca tgaaggggca gcgtttcgcc ggggccctgg tgccggagaa
    16681 gttctacaaa attccccgca cgctccgttc ctcggccggt cccgattcca agctgctcat
    16741 gatgcgcacg acgcagcggc gtcagcaggt gctcgtcatc gtccccgccg acaaggccgc
    16801 cgggttcaag gaaagcatcg gttcgcgatc gcgcgtcacg atcgtctaca cgcccgtcga
    16861 cgtgcaccgc ttcggcggaa aggcctaccc ggtgctcaag ctcgtggacg acgcgaagaa
    16921 gtgatcatcg aaggattgcg cgcgggctcc gtgccggata cacggcgcga cggcccgcgc
    16981 cgcatcagcc ccgctcttta aagaacgttc ccaccccgag ccgaaacacc gacgtcgcga
    17041 ccgacagtcc accgatcacc agccagaggg cgggatcgcg cacgggcgcc gttttaaaga
    17101 tgatgttcag atgcggcacg tacacgatgg ccgcgatcat cgccacggtg aacgccagga
    17161 acggatgcag gatcatgtta aaggaaaaca gcttctggcg aagcgatccg tcgcgcagca
    17221 tgaacacgta gatctggggc gaaagaagcg tgatcgtgaa ggacgccgtc ccggcgatcg
    17281 cgcggtcgtt cgtgagatgg tcgaccagga tatacccggc cgaaatggcg cagccgaaca
    17341 ccaggccgtc gacgatcatg tgcacccggt cgcgcagcgc gaagatcggg tccgcgaggc
    17401 gcaccggcgg ctttttcatg atgccgggcc ccgccgcatc ggtgctgatt cccaccgcgg
    17461 ggaacgcctc catgatcacg ttggtccaca ggagctggat cgcgagcagc ggcgtgggga
    17521 atcccaggat gggattgagc aggagccccg ccaccttgcc caggttgttg gtgatgagat
    17581 agcgcaccag gcgccgcagg ttctggtaca cgatgcgccc ctcgcggatg ccgttgacga
    17641 tggtggcgaa atcgtcgtcc gtgaggatca cgtcggccgc ctcctgggcc acctgcgaac
    17701 cggcgcgtcc catcgcgatg cccacgtcgg cgcgcttgag cgcgggggcg tcgttcaccc
    17761 cgtccccggt catggccacc acgtggccct tctccttgag cttgtccacg atccgcagct
    17821 tgtccagcgg cgccacgcgc gaatacacgt gtatgttctc cacctggcgc gtaaactccg
    17881 cctcggtcat cgcctcgagc tcgcgcccct cgacggcctg gctcgcgccg tcggcgatgc
    17941 ccacgctctc ggcgatcgac cggcccgtct tcttgctgtc gccggtgatc atcaccatgc
    18001 tgatgtgcgc ctctttggcc tcgcacacgg tgagcttcac ctcgtccttg ggcggatcga
    18061 agatgacggc cgcgcccagg aacacgaatc cgccgagacg cgcgcggtcg atcgtgtccg
    18121 ttccggacgc cgcgcccttg cgcgcgaagg cgatgagccg gtaacccttc tcggagcgcc
    18181 cggcgaggtc gtccaggatc cgcgcggcgt cgtgcttgcc gagcttctcc tcggcgccgt
    18241 cgacgtagtg ccggtcgcac agcgcgacga tccgctccgg ggcccccttg acgaggatgg
    18301 cccgctcacc cgcgggttcc gccaccagcg atgcggcata catgagatcg gaggaaaagg
    18361 gcacgcggtc gaccacctgt gcggactcga actgggcggg acggaagccg gcgatcactg
    18421 ccgccttgag cagcgccacc tcggtgggat cgcccaactc cttctcgacg gttccgtcgg
    18481 tctccacgat ctgcgccgtt gacgtcttga acgaggcgag gaagatgtcg cggataacgc
    18541 cggtctcccc ctcggcgatc atgcggaaga tgtcgccgcc ggtgagaaac ccatcgccga
    18601 tatagtactc cttcaccgtc atcacgtgcg cggtgatggt cccggtcttg tcggtgcaga
    18661 tgaaatcgac gttccccagc gtctcgacgg aggtgaggcc cttgatgatg atggagttct
    18721 tcgccaggcg ctggatggcc agcgacagcg cgatggtgat cgaggccgga agcccctcgg
    18781 gaaacaccgc caccatcacg ctcaccgcca ccagcactac cccgaagagg gtctcgcggt
    18841 tgaggttgtc gatgcccgtc acgcgcagca gggtgatcgc gacgaccagc gccgcggaga
    18901 cgaaggccag ccccaccagg aacttgatct cggtctgcag cttccgctgg agcggggtct
    18961 tctcctcctc gagggcctgc atgctcccgg cgatgcgccc catctccgtg cgcgtgccgg
    19021 tccccgcgac cagggcccgg ccggagccgt cgagcacgcg gctgccggcg aagaccatgt
    19081 tctccatctc gtagagcttc cggtccgtcc cggggagcgc ccctgcggtc ttgcggacgg
    19141 gatcgctctc gcccgtcagg aaggcctcgt ccacgagcag cccgtgcgat tcgatgatcc
    19201 gggcgtcggc ggggacgatg tctcccgcct cgatgatgac gatgtcgccg ggcacgatga
    19261 agcgcgcggc cacgagctcc gtcgagccat ttcgcacaac gcgcgactgg ggcgcgagca
    19321 tcttcgtgag cgcctccacc gccttctccg ccttccggtc ctgcgcgatg ccgatgacgg
    19381 tgttcaccac caccacgaac atgatcgcga ccgcctcgat cacgtcgccg atcaccagcg
    19441 agaagagcgc ggccaccagg aggatgagcg ccatgggatc ggcgagcgcg tcgaagacct
    19501 tgcggagtat cccctttcgt ttcgcgggcg ggagctcgtt gtggccgtgc tcggccagga
    19561 gcgccgccgc gcgcgcgccg gtgagcccgt cgggggagga gccgaggagc cggagcgccg
    19621 cgtcggcctc gagcgtgctg atgctgccgt atcccggcag ggacgtcgtc gttaccgcca
    19681 tgggcgcctc cgggcgtgtg gaatcatagt ggcgtgcgcg gggctcgcga ggccgcgggg
    19741 atcacgcaat gccgtatttc gccttgacct cgcgctcggc gcgaagccag tcgctcatct
    19801 cgtcgcccgg ctcgttgcgg cggatccgct cctcgaagac ctcgtaggcg tgctgccgga
    19861 tctcacttaa aaattcgtcg agttgcggaa cctgggtttt cttgggcggc atggcatcct
    19921 ccgaggcatg tgcgattacg gtgttcgcgt aacacacgat agcccagaag ggactgatgg
    19981 caataaaaaa attcacaaat cctcacgcga tccgacctcc accggccggg gcaccttcag
    20041 gcgccgcccg agcacccgct cgaaacggcc gtgcagcccg tcgccgtcga gcgacagcat
    20101 ttccgcgacg ccggtgctgc ggatccggtc gaaatagccg tcgcactccg cctcgaaccg
    20161 gcggtcgaag cgccggcgcg aatggccatg cgccgcccgc acggggccca tgcgccggat
    20221 gtcgccgtta aagtcggggc tgatatgata gagctcgtga aagatcaccc gcagcttacc
    20281 gcgcgcgtcg aggtcaaaaa accgcgggaa gtagaagtat atcacgtaga gcatgcggcg
    20341 ctccccggcg accacgcgcg gaatggcgaa ggagcgcccg cggaatcggg cgatctcggc
    20401 gccgcccgcg aagcgaagcg gcaccagctt cccgtagcac ccgccgccgc cgatcccccg
    20461 attggaggcc acgcagacga gcacgcgccc catgtcgatg tgcgcaaaca tcgggacgct
    20521 tgcgcagatg tcgcggatga tcgactcgag ctcgccggtc aggtcgatcg tccgcaggcg
    20581 cgcgggtgcg ggtgcgccgg ccatccggtc aggcgccctc ggcgcgggag accatctcct
    20641 cggcatggcg gagcgagagc tccgtgatcg tctccccggc gagcatccgg gccacctccc
    20701 gcaccttctc cgagcgggtg atgttcttga cgatcgtcgt gaagcgattt gcggtctttt
    20761 cctttttcac gatgaaatgg ctgtccgaca tcgcggcgat ctgcggcagg tgcgtgatca
    20821 ccagcacctg ccggtcgcgc gagagggtct tgagcttttt ccccaccacc tcggcggtct
    20881 tgccgccgat gccggcgtcc acttcgtcga acacaaggct ctccacgatg tccgaggcga
    20941 ggatcacgtt cttgagggcc agcatgatgc gcgagagctc gccgccggag gcgacgcgcg
    21001 cgagcgcccg caggtcctcg ccctcgttgg ccgagagaag gaactccacg cggtccaggc
    21061 cgtggggata gagcatgtag gtcttgttgt cggcctcgat gatgccgtcg ggatggatct
    21121 cgcgcttgat ggagacgcgg aacacggtgc ccgccatccc caggtcgcgg agctccttca
    21181 tcaccagctc ctcgagccgc ttcgcggtgt tcttccgccg ctcggagagg tccagcgccg
    21241 tctccttggc ctcgcgcacg gcctcccggt actcgacccg cagccgctcg atctcctcct
    21301 cgctcgagga aaaggcctcg agctcgcgca gggagcgctc gcggaactcc aggatttcgt
    21361 ccaccgtcga tccgtacttc ttcttgagcg acgagatcag cgcgaggcgc tcctcgacct
    21421 cgttcactcg ctccggcgag aagttcacgc tccgctcgta ttcccgcagc cccgccgcca
    21481 cgtcctccat ggcgtagagc gactgcttca cggactcgag cgtgtcggtg atgttcgcgt
    21541 cgaagcccgc gatggcggcc aggctctgct ccaccttctt gagcccgccc acgatgcccc
    21601 cgtcgccctt gagcagtgcg gtggccgtgc tgatctcgcg gaagagccgc tcggcgttgg
    21661 cgagcaccgt ggactcggcg cggagctcgt cctcctcgca gggcgatagt ttcgcctgct
    21721 cgatctcctt gaccgcgaag gtgaggtact cgatgcggcg ggccttctcg cgctcgtcga
    21781 tctcgaaggt gtccatcttt tccttgattt cgcccagccg cgcgtgcagg gcgcgcacgg
    21841 cgtccacgag cgccccgtgg ccggcgaagg cgtccagaag ctcgcggtgc ttcgcgatgc
    21901 gcacgatgtt ctggtgctcg ttctgcccgt ggatgtccac caggtaggcg gcgatctcgg
    21961 ccatgcgcgc gacggggatc tgcgtggcgt tggcgaagca gcgccccttc ccgttcgaat
    22021 tgagctcccg gcggatcatc agcacgccgt cgtcgcagtc gatgcccgcc tcgtcgagca
    22081 gacggcgcac gtcgggcagc ccggacacgt cgaactcccc ctcgagcacg gcccggtcga
    22141 acccggtgcg gatcatgtcg gtggtcatct tctcgcccag cacgcccgag agcgcgtcga
    22201 tgagcacgga ctttcccgcg ccggtctcgc cggtgaggat gttgagcccc ggcccgaagg
    22261 gcaggcgcag ctcgtccacc aacacgaaat tttttatgcg cagttcacga agcatgcggt
    22321 gtcgttcgcg gccggcggcc gtcacctccc ccatccaagc ttttgccgga gtatgtcata
    22381 atagctgaag tcggggtgcg tgaccagtcg aattgttgta tcggtgcccc ggaagtagac
    22441 gtcgtctccg gcctcgattc gaatagcaac ctgaccgtcg attgtcaata ataaattttc
    22501 gaagcgcgat tcgatacgcg cgcgcagggt gcaggccgag gaaacgatca tggggcgcac
    22561 ggccagcaca tgcggacaga tgggattgat gacgtacacg ctgtccaccg gcggcgcgac
    22621 gatggggccg ccggccgaga gcgagtaggc ggtggagccc gtcgtggtcg ccacgatgag
    22681 cccgtcgccg gagaaccggt tcagaaaatc cccgtcgatg gccagctcga tgtcgatggg
    22741 acgcgaaaag gcgcccttgg atatgaccgc gtcgttcacg aacacctgcc gctccatcat
    22801 ggcgccgttt ctccagtgct cgacctcgag gagcgaccgc tcggcgatct cgccgcgccc
    22861 ctcgacggcg gcggcaagat gcgggagcgc ctcgtcgggc atgaactcgg taaggaatcc
    22921 gacgcgcccg cggttcacgc cgaagagcgc cttcccggta tcggcgaaca ggcgcgcggt
    22981 gcggaggaag gtcccgtcgc cgccgatcgc gacgaccagc tccgcgtcgc ggacgaagcg
    23041 cgccatgtcg gtcacgcacc cggcgaacgg cgtcgcggag aagatatcgt agggcggtaa
    23101 aagcgtcgcc acgccccgct tccgaaggaa gtcgatcatg gagcccagga aggcgagcga
    23161 gccgtcgtcg tcggggcgga tatttgccga tacggtggcg atcatgtcag cgcttcacga
    23221 ccaggcaggg gacgggcgac ttgcgcacga ccttttcgga gaccgagccc atcagcatct
    23281 cggcgatatt gctcgcgccg tgcgagccca ggacaatgag cgtcgccttc tcgcggcggg
    23341 ccacgtcgat gatggtctta aacggcaccc cgctctcgac gatgccgcgc accgtgaagt
    23401 cgcgacccaa gcgccggcac acctcgtcga tgcgccgctg cgcgtcctgc gtgaggttat
    23461 tgaacagctc catctcgtat tgtttgatgg attcgccgaa ccacgaaacc gtctcggcaa
    23521 acgccgcatg ccgcagatcg atcacgtgca cgacgacgat ctcggcggtc cccgccccct
    23581 tgagtttccg cagataggga acggcggccc cggcgcagtc cgagaaatcc gtcggataca
    23641 gcactcgctt gaacacggac cctccgccct agaatttttt caccgcgcac atgccgggcc
    23701 ccttctcggc cagaaaggcg atcgcggccg ccagccgcgt gctgtccacc aggtaggtgg
    23761 cgtgctcgag cgtgagcagc atcgcgccgt gggccatccc ggcgatcttc agttgcgccg
    23821 ggagcttgac caggtggcgc ggcccggtga ggaccgccca gagcccctcg cggtcctcgt
    23881 agagcatgcc ccccgggaag aaggccacat ccgcgatatc gcggaacttg aacacctcgc
    23941 gcttcttctt gtcgggaacg gagagctcca cgagctgcca catcccgatg atgccggaga
    24001 cataccagag ccggttgttc tcgaaccaca tccggggcga gttcaccttg atgtccttca
    24061 tgaagagcga atcggcggcc aggtccacga tgttcgccgt gtagtcgccc gcgatgccga
    24121 ggatcaccat catcgtcgtg ccgctcttgt agagatggga ataatacggc accccgatga
    24181 agggcggctg gatcttgtcc cgccccgcca cgacggcgat cttccggtcc gcgatcgaat
    24241 agcgatgcag ttggcggccg acgaaataga acacggcgtc gtcggacacc gccagggaat
    24301 cggccttgtc ctttttatcc tgggagacga cgacgctcga ctccgatgcg ccgcccttcc
    24361 cgaagatccg cagctcgagg ccgttcgtgg ccgcgatgcg gccggttgcc ggatcgcacg
    24421 caaggtgctt gaattgcgcg ggaacgtcga tggacgccgc gcagggatga tggagccgca
    24481 ccgtattgtc cgagcgcgtg tagatcacgt tcccggaggc gacgggctcc cccttcttga
    24541 acttcgcgat cttcgaggag agcaccaggc cctcggccgc acgcagcgtc ccctcctcga
    24601 tggcgccctg ccggtaggcc tcgatgccct tgagcatctt cgattcgtgc tcgggggagc
    24661 acccgagcgc cgccgcgaac gcgacgccca caaggaatat gcgatgattc atgaagtcct
    24721 cactcgttct tggcggtctt cgccttgatc ttctccaccg ccttgtcgag gaaggtggaa
    24781 tcgtagagct cgaccatccg cgacgacgcg gacgaacggg cccggatgct gaatttggac
    24841 gtggtgatgt acatgccgct cttgcacttc tcggcgccga tcgtgcggtg gaagttgatg
    24901 atttgcccct cggtgatctc cttcgtggtg cggttgaaga cgatgaggac cggcggctcg
    24961 ttgatccgct tgatgttgaa ggccttgtac acgtactcgt tgtgcgacac ttcctttttc
    25021 gagacgatat tgtagttgag ccgcgagatc acctcctgga tgagcggctg cagctccgtg
    25081 atcgaggagg tgaagaacga tttgaggttg gggttgttca ggatgttctg gtactcctcg
    25141 agcttgagcc gggtgcttcg gaaatccgag ttgatccgcg cgatctgctc ccagtggtgc
    25201 accgcctcct tgatcttgtt ctcgatctcg aagcactccg ccagcaggta gcggtaggcc
    25261 agcgcctcgt cgtccttctt gtcgatccgc ccgatcccct cttcgagggc ctcgatcgcc
    25321 ttgtcgtaca ggccgttctc gaagtatatc tcgcccaggt ggtacaggct tttcacgaag
    25381 aaggcgcggt ccttccgcga gttctcgaac atccggatgg cggtgtcgaa gtcctttttt
    25441 cccttgtaga tgacccccag atagaagttc gcctcgaaca tggactgcgg atgctcgacc
    25501 gcgcgcagca gaaaggcctc cgcgtcgtcg tactcggaca ggtagtagct cgccacgccg
    25561 atgggcagca cgcagtcgac gagcgacgga tcggcggtga tggcgcggct ga
//
LOCUS       DAORHF010000029        25463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1222_length_25463_cov_6.076511, whole genome shotgun sequence.
ACCESSION   DAORHF010000029 DAORHF010000000
VERSION     DAORHF010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 25463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25463
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1222_length_25463_cov_6.076511"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(244..408)
                     /locus_tag="PKJ16_04310"
     CDS             complement(244..408)
                     /locus_tag="PKJ16_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46243.1"
                     /translation="MKDLMNMDDRETKQGKILGNRQKIDEYRKRISDNDYLDHAIKKI
                     ATDLSHYLTK"
     gene            complement(649..1002)
                     /locus_tag="PKJ16_04315"
     CDS             complement(649..1002)
                     /locus_tag="PKJ16_04315"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HNV46244.1"
                     /translation="MNTAIVGSAGEDAAARHLEERGFTVVARNYRYRRYGEIDLIARK
                     DDLLVFVEVKTRRSDRYGGPLRSISERKMRTMRLIAGQFLMANPRYHTRAVRCRFDLV
                     AIENGAITWVEDCFR"
     gene            complement(1289..2467)
                     /locus_tag="PKJ16_04320"
     CDS             complement(1289..2467)
                     /locus_tag="PKJ16_04320"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNV46245.1"
                     /translation="MRKISVEELKPGMRFDKPVYIDANNMLVAANVAIKEDDIKKLMK
                     WGVVQVETGGEVVSTESDIKVAQRIARTTSDDARKIIADYNNLLKKRKILIEVHRQAS
                     VAVENAHAAIRANKLFTLDEIDEALDSIIKLMSENNNIFLFLYGLEEGQNYLVAHSVN
                     VTFYSLLIGMSMKYAPNRLRELGRGTLLIDAGMVKMPLYIIHKQSNLSDHEYNLIKTH
                     PLHGYKVVKQLANVNERVALVALQHHEQFDGKGYPRGLTGNQIDEYARIAAIADSYEA
                     QISNRAYRKKQYFYHAMRNLLSSGINRFDPVLLRVFLSRMSVYPIGSLVELNDGCVGL
                     VVGSIPQKPLRPFIKLIFDNQGKRLEETTILNLLEESALYIVKALDESEAGINILDVL
                     "
     gene            complement(2460..2762)
                     /locus_tag="PKJ16_04325"
     CDS             complement(2460..2762)
                     /locus_tag="PKJ16_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HNV46246.1"
                     /translation="MKRPAAAAIGYAGADSLPRVLAAARGALVARLLEMAAAHDVPVY
                     RDADLAEVLSRLEAGSEIPEELFRAVAEVLAYCYRVNGAFANKMDAIMRIKAGPDA"
     gene            complement(2763..3833)
                     /locus_tag="PKJ16_04330"
     CDS             complement(2763..3833)
                     /locus_tag="PKJ16_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46247.1"
                     /translation="MRIDRHDAIRIPAGGVRPGQLSRLQAGDLVWGKIVQRSGPRQAL
                     LDVAGTRVSAEFTSGVPREGRVLLVLTGHDGNALLFSRVKKDDPRYYRDLFHLTVFDA
                     SRIGKKELGGILARLRDGAGGLFELSASIARSRTGKENSASRPGGLLRALAALGVRDA
                     DLPETAFLLASLRNRHLAAARALLRGIGYGGAGDAVSAPPGGDSAIDAAGLLRPIEDA
                     LDRDGDAPRHLHDLLCAVLGDEDDALLEGEFAYEDDGPRAVRYCAGEDGCVVSIELSR
                     LGPVEVLVRRDGSALRCIISCDTEEARAALAEGADELHTTLQQVMNTAVRVDIYKRSE
                     IAQKIIEINALSTIHSVFDARV"
     gene            complement(3823..4557)
                     /locus_tag="PKJ16_04335"
     CDS             complement(3823..4557)
                     /locus_tag="PKJ16_04335"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HNV46248.1"
                     /translation="MPRRALGRRAPTFDLERAHAERGCALIAGVDEAGRGALAGPLAV
                     GLVVYPTHLFSEPPDGRLSGVRDSKQLTPRQREKAETAVRAAAQVALTAFVSHRIIDK
                     INVNRATEAGLRKLLRMLPRTPDIILMDGTFSFDVGVPIVPIVGGDSRSMSIASASIL
                     AKVARDALLRGLDARYPGYGFHENKGYGTRAHRTAIEMIGPSPVHRLTYDPVRSLREG
                     VVVEHEGSEPRNTTRIAGKGSEPHAD"
     gene            complement(4563..4982)
                     /gene="rplS"
                     /locus_tag="PKJ16_04340"
     CDS             complement(4563..4982)
                     /gene="rplS"
                     /locus_tag="PKJ16_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HNV46249.1"
                     /translation="MDLIKAIEQELMPVQRTLDIEVGDTVRVHYKIKEGTRERVQIFE
                     GIVIAIDNKGISKTFTVRKISFDIGVERIFPFFSPRIGAIDIVRKGKRRRAKLYYLRE
                     RTGKSARVKERRVKGKTGAQEPVVPVEGTAPATEGAE"
     gene            complement(4960..5685)
                     /gene="trmD"
                     /locus_tag="PKJ16_04345"
     CDS             complement(4960..5685)
                     /gene="trmD"
                     /locus_tag="PKJ16_04345"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HNV46250.1"
                     /translation="MIALKIITLFPEFFDGPLRAGLMGKAIESGIIALEVMDLRDFSE
                     DRLRRCDDYPYGGGSGMVLTPGPLFRAIRAARTPRTRVILTTPAGRLLTQDLVKELSG
                     EAELCIVCGRYEGVDQRVIDRCIDDEISVGDYVLSGGEYAALIIADAVARYVPGFMGN
                     AESLVDESFADGLLEYPQYTRPAEYEGMQVPEVLLSGHHGNVAAWRRDQRIEKTRRLR
                     PDVYSNYLMRNNRGNDHGSHQGD"
     gene            complement(5696..6223)
                     /gene="rimM"
                     /locus_tag="PKJ16_04350"
     CDS             complement(5696..6223)
                     /gene="rimM"
                     /locus_tag="PKJ16_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HNV46251.1"
                     /translation="MSGAEYLRIARVLGAHGLGGRIKILLVTDVQERFSVGGVVYLRQ
                     ANEFRRHVVEEFLPYKKNVFLLRLDGIGVREAVDALRGCELFIPREDAEESRHLLGGD
                     EFYYYDVIGCAVYRDGAPFGAVSGIMEAGAGEILVITDGTGREHLVPFVREMVNTDRL
                     PERRIDITPVEGLLE"
     gene            complement(6220..6456)
                     /locus_tag="PKJ16_04355"
     CDS             complement(6220..6456)
                     /locus_tag="PKJ16_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013606980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HNV46252.1"
                     /translation="MDYRGLVEFMVKSLVDHPDEVELREIEGEKSTILELKVTKDDIG
                     KVIGKHGRIARAIRTIINASATKSGKRVVLEILD"
     gene            complement(6475..6738)
                     /gene="rpsP"
                     /locus_tag="PKJ16_04360"
     CDS             complement(6475..6738)
                     /gene="rpsP"
                     /locus_tag="PKJ16_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HNV46253.1"
                     /translation="MAVKIRLQRVGAKKKPCYRVVAIDERKKRDGAVLEFLGQYQPIS
                     GGSQFSVNEDKVIGWLKNGAQPTLTVANLLKKNGVWQKYKSAR"
     gene            complement(7031..7711)
                     /gene="rpe"
                     /locus_tag="PKJ16_04365"
     CDS             complement(7031..7711)
                     /gene="rpe"
                     /locus_tag="PKJ16_04365"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HNV46254.1"
                     /translation="MKDTASRVRISPSIIATDLTTIGVDAAGYDPAVVDLLHVDVMDG
                     SFVPNLTIGPGFVKSLAAHTDIPLDVHLMIERPELSVESYLALNPEIVTIHYESTRFP
                     ARLMRLIRERGAVPGVSINPATPVESVFDILPYAGLVLVMSVDPGFYGQSFMPEALRR
                     IERLAAFLSANGLDGSVDIQVDGGINADTIAAVVRAGARIVVAGAAAFKGGDVNAAVA
                     ELKRRAIS"
     gene            complement(7695..8726)
                     /locus_tag="PKJ16_04370"
     CDS             complement(7695..8726)
                     /locus_tag="PKJ16_04370"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HNV46255.1"
                     /translation="MAQDTQTGGAPETKFSYFKSVRHIAFIFFIGFSFYLLISSIMLV
                     FLTRPDKEVKVPHVVGKQFGDIYNSLSNKGLRPDVRFYDVFDVDDGFILSQHPSAGSI
                     VAEGSRVKLLVSRSALHLTVPNLVGMELPFAVNKLRNQHVHDRTISVATGIVSYVPSE
                     KSAANIVIDQSPKSGERITPGRRINLLVSAGKVDADMKMPEVTGQSIDLCIDLLLSKG
                     VMVSQDIATVATPEQSGIVVSQTPAKDAPVKKGDIAVLRVNHFPMKERSYNAYDRIDW
                     NIPSDEKEALYEAVIEDFGPKRIAFSRRMRPGEKISFVFSRTGDATIRILADKKSIKS
                     FGVKFEGYR"
     gene            complement(8815..10167)
                     /locus_tag="PKJ16_04375"
     CDS             complement(8815..10167)
                     /locus_tag="PKJ16_04375"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HNV46256.1"
                     /translation="MGHILIVDDNPPILDYIADILTKSGHTTVACADGGEALAVFMRQ
                     EFDLVISDLFLPVLDGFELLAKIRTHDAEMPVIIITGQGGVDDAVKAIKNGASDFISK
                     PFQPLEFRLKVEKNLEHAALKKELARIKEQGAVLSGGVSMVGESPVMKKLITRVKRVS
                     RANATVLILGESGTGKELLARAVHEESERHAKPFVPIDCSTLTETIIESELFGHVRGA
                     FTGADRAKKGLLEEANGGTVFLDEIGNLSPTIQAKLLRFLQEREVKPVGSSRSVRVDV
                     RIICATNVNLKEEMRRGAFREDLYYRLAGIELVIPPLRERLEDLPFLIGHFIRKYAGD
                     LNKKVNFIEEQALEILRNREWKGNIRELEHLIEYALIVENSDRITTGTILHILPEKSE
                     GVAAEDFDPDLEATMARLEAAHIRSVISMAGNNRSKAAKLLGISRSVLYEKLRRHGIG
                     "
     gene            10368..11474
                     /locus_tag="PKJ16_04380"
     CDS             10368..11474
                     /locus_tag="PKJ16_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV46257.1"
                     /translation="MIKILLTADLHWGTTRDGLASLNACRAKTFERIMSLAATHDLLL
                     VAGDLFDGLGTDDPAYRAAAERFGAVLSGGTAIALIPGETECGDDGAPLPVIDTLPAS
                     RVFSLTDEGPVRLERGGEIVHVYGAPARMPFDPARYARTNDDGFHIGLFHGNFTVKED
                     GDGAPVHTLTRELIRECNLDFFAVGHTHGFRIFKQQNRVIGASPGSPEAAARTETGER
                     SVLSIALENNEITSIRRVIVNTAELRALELDCGACASDDALFDELAKNKSPVVHCTAR
                     LVGRREFFIDPARLDQLRREFLSLDITDESAPGLGALLGEFSRENSLRGEFFSLLDDM
                     RRRSALPADEDETHLRSVLESLGRTGTYRDEDWR"
     gene            11471..13618
                     /locus_tag="PKJ16_04385"
     CDS             11471..13618
                     /locus_tag="PKJ16_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46258.1"
                     /translation="MKITGVTIAKPGLFDNTRLAPGEGVTVIYGRSGSGKSAIARALI
                     DVLGAGMEADGSPAHSWENLFVEVSLASTHGSFRFVRNGERSFEIASVEGSGEPREIV
                     HDDQIGRNPDAARLRIREALLAHANGHASLALLASGSRAYLTTIAYLAAPVDTLSAPA
                     LHGESLRRLLLDDATRFYGLSQALNAAFGPSASAEGIEAELRRDAVELRDMEKRVELI
                     DIQREKFDKLHREMALVEREIDELEGAASRLRDRVAMLGAVRDLLERREVLRARIADI
                     RAQLDEEASNREAAERLAAEIADAYPQFANFDEAKKRNLGEIQERFTDIRDINETIAE
                     YHDALAARTKKLKIVSTALIAASFAALVLAVTRTGIALAPSKIITMGAILGGLSAVSL
                     AALGLLATLGKRTRAITTLLNRKAAAEERLQRILNENNLPIRDLTLASLYEYLLQYFN
                     DFNNFTEKQLELFRIRESMQGPACARVLEHELEGALADERALGAEIDAVTAELGGAAD
                     NGSLQDAIDAIVVETASLEESINEKRAVMRQVTDEILGQEDRGAERAALLAEIAARRE
                     RHERHARHVETMRFVRGVLDEAVRRREEKQRYRLINRAAERFHTLTDNQYISAIDREH
                     VRALVAGDALPAGTSQVVEHLMLIAVKLALTEFMIDAGGALPLILDEPFLYMDDRRAA
                     RFRELLEATAPMRQVILLTHNNLYKDWGTYIEL"
     gene            13620..14606
                     /locus_tag="PKJ16_04390"
     CDS             13620..14606
                     /locus_tag="PKJ16_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46259.1"
                     /translation="MDRNGQLTFSDDPLLPRLNEAYRLIEDGSFHEAVAIFDELMDAN
                     PDYPGVPEGYRVARFWNNRAVEMTQEAEGKKKADYLMRQWKDFSKYAGEKQIADSAAA
                     RAAMKYIFQVASRQYRIAFQRGEGAADNFELLLNLGYCLVTLGDHKNVIDTLEYARSS
                     YRASARLLAILGESYFHTGDVPKSLLYFREAFFLDPSEIDLSIVKAKPVTDLLQTVRT
                     ERPGCADPREWAPVYGYLQDVFYVRRQLGSHQIESIKKDIYTLETNFQTLSRDRIENS
                     NITPRLINKYLWMLDYFEYQNYDFQSVTEIRQRLVAIDKRLFEEHFKREKKK"
     gene            14731..17382
                     /locus_tag="PKJ16_04395"
     CDS             14731..17382
                     /locus_tag="PKJ16_04395"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HNV46260.1"
                     /translation="MDFPSTYNPAEAETKWYERWERERRFHGDVTDGNEPYSIVIPPP
                     NVTGNLHMGHALNNTLQDILIRWKRMQGKSAMWMPGMDHAGIATQNVVERLLREEGKT
                     KNDLGRAAFEKRVWEWKEHSGGQIQNQLKRLGCSCDWERERFTLDEGLSRAVRKVFVT
                     LYQEGLIYQGERIINWCPRCETALSDIETEYQDLQGKLYYIKYPVKGSSDLITVATTR
                     PETMLGDTGVAVNPEDERYKHLIGTMLILPLVNREIPIFADAFVAQEFGTGLVKVTPA
                     HDPNDFEMGKRHDLPEINILDKNANINENGGPYKGLTRFEAREKVVKDLDALGLLVKI
                     ENHDHSVGHCYRCSTVIEPYMSKQWFVKIKPLADEAIRAVENGSIRFVPTNWSKTYFE
                     WMYNIRDWCISRQLWWGHRIPAFYCRDCGHITVEMEDPSQCARCGSTRLAQDEDVLDT
                     WFSSALWPFSTMGWPDITDTLKKYYPTSVLVTGFDIIFFWVARMIMMGLKFMKDVPFR
                     DVYIHALVRDEHGHKMSKSRGNVIDPLIMMEQFGTDAFRFTLAIFAAQGRDIIMSEKR
                     IEGYRAFCNKLWNASRFIVMNLGDDFAPAPIDTKALEPFDRWILHRLNDAIADITRAL
                     DEYKFNEAAQAVYDFLWRELCDWYLELSKQRLYAKEAGMAASSETARQVLYHVLATAL
                     RLLHPFMPFITEELWRIISPQGEMLITASWPAPDPAFNFAYEAAETEIFKEMIYKIRN
                     VRGEMNIAPDKKLNAVFKTGSDAISSIVNREAANIRALARVNEISVDAAYAPDKTDAA
                     AVMKDVEIYLPLTGIIDFAVERARLEKEIARVSGELEKVEKKLGNPSFIEKARPDVIE
                     KEKAKSAEMADLLAKLRGSLEKLPSQD"
     gene            complement(17514..18308)
                     /locus_tag="PKJ16_04400"
     CDS             complement(17514..18308)
                     /locus_tag="PKJ16_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV46261.1"
                     /translation="MSTTILMIHGMMTTAICWMAYRGFFEKLGYRCLVPTLRHHEMRP
                     DRIPDPRLSETGLLDYVDDMEQLIQGLPEPPIVLGHSLGGLIAQILIGRSRVRVSAGV
                     LITPAPPQGVFLFTRRNLRTLAGIVLRPRWWRSACRLSSAATSYGVFNRMPIGMHERL
                     HSFIVPESGRTLLQVGFPVFDSEKASRVDGRGMTVPLLVIGAAHDNLVPSSVVKRIHG
                     KYRANAVYREYDNHAHFIISEPGWERVAGDIHAWLEGLAGRRHGRE"
     gene            18560..20098
                     /locus_tag="PKJ16_04405"
     CDS             18560..20098
                     /locus_tag="PKJ16_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV46262.1"
                     /translation="MKIGIVILLLCCLGCGYSSAERVASWQALFDDASADPGAIATRT
                     DWTPIALPSMFRLPYPPARTFRYLWLRGAANIAGDPSQYAGIRLGRIYYIDRTYVNGI
                     LVGSQLTEEYNAVHNPRAYAIPKGVLKTGKNEVLIRLGIYGTEYGGLLDEVLLLGPRD
                     FIGARISDEFIYRQLPIGIALFLLGQMAFNMIFFIWRKKQRANLYAAAMCLIWSLYIF
                     ALFSPYFPFTDDFRITFLWSCTSFVPILFLMLIQSFYKVYLSYFNRVVIPLLLAVTGI
                     VLAFQDTTAAFYPGPILGTGTLFAVIPLLSYVMYRVNSRRPDPMIFVFIGFGLLPGLF
                     IGWDIVNYLWVHHYPPLTHTYTIPVFIVGVMILIIREFIKKEIELDLLYRKLEEPSSE
                     EPVKKPGITSSTEGKLEKIIEFLKENYASDISREGLAGAMNLSPDHMSRMFKAYTGKK
                     INDYINELRITDAAGRLADPEVKIIDIAFAVGFESLATFNRSFLKVTGKTPTDFRAAL
                     KKDD"
     gene            20190..22415
                     /locus_tag="PKJ16_04410"
     CDS             20190..22415
                     /locus_tag="PKJ16_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46263.1"
                     /translation="METGTLDRKLLLGFPLRVFLSTDLITYLVSIPVTVFISYLVLPM
                     EGTQRIVFFSVVGAVVLIAVNLTFFQTRALFAPVTKYFTKLLDDEPTTDAEYVAAWNC
                     FFTAARRRAIESIIAWTVLMPVAIIVFTVFFHPTLTSRIVIGFLLLANIIFAGVYYYL
                     VIDSQVRKIARLGIFSRRMEGCNIARRPLSLTIAIIMLGMVAFFCAVVIPVIFAITNS
                     FQERAYLSEMKIVITLIDADLEKRFDERRAPRGAIAPDGADPIAHIQDLVKRTRVGET
                     GYPFFLDDRNAVIGHPDPKQIGQNIDRFDFGRRLIALPSGSHFRYLWQDRYKIMYFVK
                     NKKYGFTSAITAYLFDIERHSLYVVAIMCLFLVAGFICTGLCSYWFVATRLRPLSRCR
                     QLIDEMGNGNLSVEFTGVTNDEIGIIMNTLNDFISRLRDVIRNIQGIAHEMASSAEEM
                     SSAALSFSENAQNQAASAEEGSATSEEVASGVEHIADGAERQLASLNALIGEINELAG
                     SITEMGERIEQTVHINEDISRKATSGDEALRTMNASMQRITDSSHEMTNIVKIISDIS
                     EQINLLSLNAAIEAARAGEAGRGFAVVADEISKLADQTASSIKEIDKYIKSSNDEVGR
                     GMANASSTSQLIMSIIQGVAQISGMMAELADRMRRQTAINRSVTENAETVRNRAEEIS
                     LATDEQKRAMDEIVRTITHINELTQANASGAEEMAGNTEGLASMAEALKASIDFFRAS
                     E"
     gene            complement(22424..23524)
                     /locus_tag="PKJ16_04415"
     CDS             complement(22424..23524)
                     /locus_tag="PKJ16_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46264.1"
                     /translation="MTGPVTPLIELSGVTVHRGGVRVLDVSSLAVAAGETLGLVGPNG
                     SGKTSLLLTLATLLGTTTGTISFRGAPVATRRDRLAYRRRCAFVFQEPLLFSGSVADN
                     VASGLRMRGMGRAEIARRVDETLHRLRIDHLRGRAAQKLSGGEAQRTSLARAFATAPD
                     LIFLDEPFSSLDANARESLVADLAGTLRETGATAVIITHDAQEALALTDRIAVMERGR
                     IVADGPADDMFNNPVSEFMASFAGMETVLVGRVASTRGGAAVVDVSGHAIETAVAAEH
                     GSAVALCIRPENVSLSVGPARAGARSSVRNSFPARVVDVYRKGVFFKVRLDCGFPLVS
                     HVTADSLERLRLAPGRQVAASFKATAVHVIRK"
     gene            complement(23521..24219)
                     /locus_tag="PKJ16_04420"
     CDS             complement(23521..24219)
                     /locus_tag="PKJ16_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV46265.1"
                     /translation="MGIFLDGIWQAFYLLVTLDPEVLRITLLSLQISGSATLISMLLG
                     ISSGAAVALAEFPGKRVVISLINTGMGLPPVVVGLFVTMFLWRNGPFGFLEILYTPVA
                     MVIAQAVIAVPIVTGITIAAMQNLPPNLRLQILSLGATRIQMVWILMKEARLPLLAAV
                     MAGFGGVISEVGASIMVGGNIKGFTRVLTTATVMETGKGNFELAIALGIILLLLAFLI
                     TGVLTIIQQREKRR"
     gene            complement(24220..25041)
                     /locus_tag="PKJ16_04425"
     CDS             complement(24220..25041)
                     /locus_tag="PKJ16_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV46266.1"
                     /translation="MALLAGSWGASPAAAQKVKSVILATTTSTQDTGLLDVLIPIFEK
                     KTGFMVKTIAVGSGQAMAMGRKGEADVLLVHSPDDEKAFMKAGFGARRRLVMHNDFII
                     VGPAADPAKIKGTKKSADALKKIAKSESLFVSRGDNSGTHAKEKNLWKAAAVKAEGQK
                     WYQQTGLGMGQTLAVAAEKKGYTLADRGTYLALKKKLGLDILVEGDAKLLNIYHVIEL
                     IGKNFPKVNVAGGTAFADFMVAKETQKIIKTFGVEKFGSPLFFPDAGKRVESLGK"
BASE COUNT         4838 a         8263 c         7761 g         4601 t
ORIGIN      
        1 gatatcgaac accggccgga gcgcaatgta gaagatgagc gcgaacagga agggccacac
       61 aaaatgccgg tagatgagcc cgaccaagag cagcaccatc ccgaacagaa tgaaaaacag
      121 cgccatcagg cgtttgttct ccatcacggc gcgatagggt tccttgttct gcagcatgcg
      181 ggcctcctcc gggcgcacca cgggcctcgg cggtgaaaac gggcgagtcg gttgtttcgt
      241 tcgctacttc gtcagatagt gagataggtc cgttgctatt ttcttaatag catgatccag
      301 gtagtcattg tccgagatcc tcttccggta ttcgtcaatc ttctgccggt ttccgagaat
      361 cttgccctgt tttgtctcac gatcatccat gttcatgaga tctttcatga aagctccctc
      421 tccttggccc gccgttcctc catgaacggc gcccagcgac ctgattaatt catcggactc
      481 ggggggggag acttaagcag ctttttgcgc ctgcgttaaa tatttagccc aattcggccg
      541 gattatgaaa cattgggggc caaaaaaatc atcgaaatat agaaaatgtt tacttcggaa
      601 aaagggtgat gatagcgccg gaaaacgaac gaaatggcaa aaaccgtatt atcggaaaca
      661 atcctcgacc caggtgatcg cgccattctc gatcgcgaca agatcgaagc ggcacctgac
      721 ggcgcgggta tgatagcgcg gattggccat caggaactgt ccggcgatga gccgcatcgt
      781 gcgcatcttt cgctccgaga tcgaccgaag cggccccccg tatcgatcgc tccgccgcgt
      841 cttcacctcg acgaacacga gcagatcgtc cttgcgcgca ataaggtcga tctcgccata
      901 gcgacgatac cggtaattcc gcgcgacgac cgtgaagccg cgctcttcga gatgtcgcgc
      961 cgccgcgtcc tcgccggcgc tcccgacaat cgcagtattc atgaccgcgg accttcctac
     1021 aatgcggcgt tgcgcggggc tcatcccccg cttgttgatc aatgcgagcg gagcgtaagc
     1081 gcgcacgagc cctcgtatac aaatacgaat gctgcatacg cttctttgaa cgggaaatgc
     1141 ggggaatcgt gaatgtgcgg catcactgac cgaacgcggc cggtcacgac gcatcgaacg
     1201 ggacgcagcc atcataacaa tgtgcgtaat ttaaacggcg atggtcggga tttagaacct
     1261 actaaaatag gctttaaaaa agggaaaact acagcacgtc gaggatgttg atgcccgcct
     1321 cgctctcgtc gagcgccttg acgatataca gcgccgattc ctcgaggagg ttgaggatgg
     1381 tggtttcctc gagccgcttg ccctggttgt cgaagatgag cttgatgaac ggccgcagcg
     1441 gcttctgcgg aatggagccc accaccaggc cgacgcagcc gtcgttgagc tccaccagcg
     1501 agccgatggg gtacaccgac atccgcgaca ggaagacgcg caggagcacc ggatcgaagc
     1561 ggttgatgcc gctggagagc aggttccgca tggcgtggta gaaatactgc ttcttccggt
     1621 aggcgcggtt ggatatctgc gcctcgtagc tgtcggcgat cgccgcgatg cgcgcgtact
     1681 cgtcgatctg gtttccggtg agcccgcgcg ggtagccctt gccatcgaac tgctcgtggt
     1741 gctgcagcgc gaccagcgcg acgcgctcgt tcacgttggc gagctgcttc accaccttgt
     1801 agccgtgcag cgggtgggtc ttgatgagat tatactcgtg gtccgagagg ttcgactgct
     1861 tgtggatgat gtagagcggc atcttcacca tgccggcgtc gatcaggagc gtcccccgcc
     1921 ccagctcgcg cagccggttg ggcgcgtact tcatggacat cccgatgagg agcgaataga
     1981 aggtcacgtt caccgagtgc gcgaccaggt agttctgtcc ctcctccagc ccgtagagga
     2041 acaggaagat gttattgttc tcggacatca gcttgatgat gctgtcgagc gcctcgtcga
     2101 tctcgtccag ggtgaagagc ttgttggcgc ggatggcggc gtgtgcgttc tccacggcga
     2161 cgctcgcctg ccggtgcacc tcgatgagga tcttccgctt cttgagcaga ttgttgtagt
     2221 cggcgatgat cttgcgcgcg tcgtcggagg tggtgcgcgc gatgcgctgg gccaccttga
     2281 tgtccgattc ggtggagacg acctcgccgc cggtctcgac ctgcacgacg ccccacttca
     2341 tcagcttctt gatgtcgtct tccttgatgg cgacgttggc cgccaccaac atgttgttgg
     2401 cgtctatata gaccggtttg tcgaagcgca tccccggctt gagttcctcg acggagatct
     2461 tacgcatcag gccccgcttt tattcgcatt atcgcatcca ttttgttcgc aaacgccccg
     2521 ttcacccgat agcaatacgc aaggacctcc gccaccgcgc gaaagagctc ctcggggatc
     2581 tcgctcccgg cctcgaggcg agagagcacc tcggccaggt cggcgtcgcg gtacaccggg
     2641 acatcgtgcg cggcggccat ctcgaggagc cgtgcgacca gcgcccctcg cgcggcggcg
     2701 agcacccgcg gcaggctgtc cgcgcccgca tatccgattg cggcggcggc gggtcgcttc
     2761 atttacaccc gtgcgtcaaa cacgctatgt atagtcgaca aagcatttat ttcaataatt
     2821 ttttgagcga tctcgcttct tttatatata tcgacacgaa ccgcggtatt cattacctgt
     2881 tgaagcgtcg tgtgcaattc atccgcccct tcggcaagcg ccgcgcgcgc ctcctcggtg
     2941 tcgcaggaga tgatacagcg cagcgcggaa ccgtcgcgcc gcaccaggac ctcgacggga
     3001 cccaggcgcg agagctcaat cgagacgacg cagccgtcct cgcccgcaca atagcgtact
     3061 gcgcgcggcc cgtcgtcctc ataggcgaat tcgccctcga ggagcgcgtc gtcctcatcg
     3121 cccagaacgg cgcacagcag gtcgtgcaga tgtcgcggcg catcgccgtc cctgtcgagc
     3181 gcgtcctcga tgggccgcag cagccccgcc gcgtcgatgg ccgaatcgcc gcccggcggc
     3241 gcactgaccg catcgcccgc gcccccgtag ccaatccccc ggaggagcgc ccgcgccgca
     3301 gcgaggtgcc ggttccgcag cgaggcgagc aagaacgcgg tctccggcag atcggcgtcg
     3361 cggacgccaa gcgccgcgag cgcccgcagc aggccccccg gccgggacgc tgagttttcc
     3421 ttgcccgtgc gcgaacgggc gatggacgcg ctcaactcga acaggccgcc cgcgccgtcc
     3481 ctgagtcggg cgaggatccc gccgagctcc tttttgccga tgcgcgacgc gtcgaacacg
     3541 gtcagatgga agaggtcgcg ataatagcgc ggatcgtcct tttttacgcg ggaaaagaga
     3601 agcgcgttcc cgtcatgtcc cgtcaggacg agcaataccc ggccctcgcg cgggaccccg
     3661 ctggtgaact cggcgctcac gcgcgttccg gcgacatcga ggagcgcttg tcgcgggccg
     3721 ctgcgctgga cgatctttcc ccatacgaga tcgcccgcct ggagccggga gagctgcccg
     3781 ggccgcacgc cccccgccgg gatccggatg gcgtcatggc gatcaatccg catggggctc
     3841 tgaccccttc cctgcgattc gcgtcgtgtt gcgaggctct gacccctcat gttccaccac
     3901 caccccctcg cgcaggctcc gcaccgggtc gtaggtgagc cggtgcaccg gagacgggcc
     3961 gatcatttcg atcgccgtcc ggtgcgcgcg ggtcccgtac cccttgttct cgtgaaaccc
     4021 atagccggga tagcgcgcgt ccagtccgcg cagcagcgcg tcgcgggcga ccttcgcgag
     4081 tatcgacgcg gaagcgatgg acatcgagcg cgaatccccg ccgacgatcg gaacgatcgg
     4141 aacgcccacg tcgaacgaga aggtcccgtc catgaggatg atgtcgggcg tgcggggcag
     4201 catgcgcagg agcttgcgga ggccggcctc ggtggcgcga tttacgttaa ttttgtcgat
     4261 gatacgatgc gacacgaacg cggtcagtgc gacctgcgcg gcggcgcgca cggccgtttc
     4321 cgccttctcg cgctgacgtg gggtgagctg cttggaatcg cggacgccgc tcaggcggcc
     4381 atcgggaggt tcggaaaaga gatgtgtcgg atagacgacc agccccaccg cgagcggccc
     4441 ggcgagcgcc ccccgcccgg cctcgtcgac cccggcgatg agcgcgcagc cgcgttcggc
     4501 gtgcgcccgc tcgagatcga aggtgggcgc acggcggccg agcgcgcggc gcggcacgga
     4561 tgctactcgg cgccctcggt ggcgggagcc gttccctcga ccggcacgac gggctcctgc
     4621 gcgccggtct tccccttcac gcgacgctcc ttgacccggg cggacttgcc ggtccgctcg
     4681 cgcagatagt agagcttcgc gcggcggcgc ttgcccttgc ggacgatgtc gatcgcgccg
     4741 atgcgcggcg agaagaacgg gaagatccgc tccacgccga tgtcgaagga tatcttccgc
     4801 acggtgaagg tcttgctgat ccccttgttg tcgatcgcga tcacgatccc ttcgaatatc
     4861 tggacgcgct cgcgggtgcc ttccttgatt ttatagtgga cccgtacggt gtcgcccacc
     4921 tcgatatcga gggtgcgctg caccggcatc agttcctgtt caatcgcctt gatgagatcc
     4981 atgatcgttt cccctgttgt tcctcataag atagttgcta tagacatcgg gccgaagccg
     5041 tctggttttt tcaatgcgtt gatccctacg ccatgccgcg acattgccgt gatggccgga
     5101 gaggagcacc tccggcacct gcattccctc gtactcggcc ggccgcgtgt actgcggata
     5161 ctcaaggagc ccgtccgcga agctctcgtc aacgagcgac tcggcgttcc ccatgaatcc
     5221 cggcacatag cgggcgacgg cgtcggcgat gatcagcgcg gcgtattcgc cgccggagag
     5281 cacgtaatcg cccaccgaga tctcgtcgtc gatacagcgg tcgatcaccc gttgatcgac
     5341 cccctcatag cgcccgcaga cgatgcacag ttccgcttcg ccgctgagct ccttgaccag
     5401 gtcctgggtg aggagccgtc ccgccggcgt ggtgaggatc acccgcgtcc gcggcgtgcg
     5461 cgccgcgcgg atcgccctga agagcggccc cggggtgagc accatcccgc ttccgccgcc
     5521 gtagggataa tcgtcgcagc gccgcaggcg gtcctccgag aagtcccgca ggtccatcac
     5581 ctcgagcgcg atgatcccgg actcgatcgc cttccccatg agccccgccc gaagcgggcc
     5641 gtcgaaaaac tcggggaaga gcgtgattat tttaagcgcg atcacaccga ccccgctact
     5701 cgagaagccc ctcgaccggg gtaatgtcga tccggcgctc cggcaggcgg tcggtgttga
     5761 ccatctcccg cacgaagggc accagatgct cgcgccccgt gccgtcggtt atcacgagga
     5821 tctcgccggc gcccgcctcc atgatgccgg acacggcgcc gaagggcgcg ccgtcgcggt
     5881 acaccgcgca cccgatcacg tcataataat aaaactcgtc gccgcccaga aggtgccggc
     5941 tctcttcggc gtcctcccgc ggtataaaca gctcacaccc gcgaagcgca tcaaccgcct
     6001 cgcgaacgcc gatgccgtcc aatcgaagca aaaaaacgtt ctttttatag gggaggaatt
     6061 cctccaccac atgccggcga aattcgttcg cctgcctgag atacacaacg ccgcccacgg
     6121 aaaaacgctc ttgtacgtcg gtcacgagca ggattttgat ccgtcccccc aggccgtggg
     6181 cgcccaggac gcgggcgatg cgcagatact ccgctccgct caatcaagta tttccagcac
     6241 gacccgcttc cccgacttcg tcgccgacgc gttaatgatg gtgcggatgg cgcgcgcgat
     6301 ccggccgtgc tttccgatta cctttccgat atcgtccttg gtgaccttca actcaagaat
     6361 cgtggatttc tcgccctcga tctcacgcag ctcgacctcg tcgggatgat cgacgaggga
     6421 tttcaccata aactccacga gtcccctgta atccattccc gtctcctcga gcgcttaccg
     6481 ggcgcttttg tatttctgcc acaccccgtt cttcttgagc aagttggcga cggtgagcgt
     6541 gggctgcgcg ccgttcttga gccagccgat caccttgtcc tcgttcaccg agaactggct
     6601 gccgccggag atgggctggt actgtccgag gaactcgagg accgccccat cacgcttctt
     6661 gcgctcgtca attgccacca ctcgataaca gggcttcttt tttgccccga ccctctgcaa
     6721 gcgaatctta actgccactg aaaccgcacc tccagaaaaa agtaagggaa aacctatttt
     6781 ttgacacttt tttgtcaata taaaatatta ttatttcccg caggcatggc gcgcggggtg
     6841 cgccgatggc gtctccgagg tcactacacg acgtcgcgcc gatgcgcgtg cgcgcgggcc
     6901 aatacgacat aatgcgccga cgcagaacaa acaaaattcc gtcgcggtgc gaacgcagcg
     6961 cgcccgcatc gccgccatcg cgcagccgcc gcagcgatcg cgagagaacg gaatgggatg
     7021 attggggacg ctaggagatc gcccgacgct tgagctcggc gacggccgcg ttcacatcgc
     7081 cgcccttgaa ggccgccgca ccggcgacga cgatgcgcgc gcccgcacgc acgacggcgg
     7141 cgatggtgtc ggcgttgatg ccgccgtcga cctggatgtc gacggagccg tcgagcccgt
     7201 tggccgagag gaacgcggcg aggcgctcga tccggcgcag cgcctccggc atgaatgact
     7261 gcccgtagaa tccggggtcc accgacatga ccagcaccag cccggcatac gggagaatgt
     7321 cgaacaccga ttcgaccggc gtggccgggt tgatggaaac gccgggcacg gccccccgct
     7381 cgcgaatgag ccgcatgagg cgcgccggga agcgcgtgct ctcgtagtgg atggtgacga
     7441 tctccgggtt aagcgcaagg tacgattcga ccgacagctc ggggcgctcg atcatgaggt
     7501 gcacgtcaag cgggatgtcc gtatgcgctg cgaggctctt cacgaagccg gggccgatgg
     7561 tgagattggg gacgaagctc ccgtccatga cgtcgacgtg caacaggtcg accacggcgg
     7621 ggtcgtaccc cgccgcatca acaccgatgg tggtgaggtc ggtcgcgatg atcgacgggg
     7681 agatgcgcac ccggctagcg gtatccttca aacttcaccc cgaaggactt gatggatttc
     7741 ttgtcggcga ggatgcggat ggtggcgtcg cccgtgcggc tgaagacgaa cgagatcttc
     7801 tcgcccggcc gcatgcgccg agagaaggcg atgcgcttgg gcccgaaatc ctcgatcacg
     7861 gcctcgtaga gcgcctcctt ctcgtcgctg gggatgttcc agtcgatccg atcgtaggcg
     7921 ttatacgagc gctccttcat ggggaaatgg ttcacccgca gcaccgcgat atcgcccttc
     7981 ttgacggggg cgtccttggc gggcgtctgg gacacgacga tcccgctctg ctcgggcgtc
     8041 gcgacggtgg cgatgtcctg actcaccatc acgcccttcg acagcagcag atcgatgcac
     8101 aggtcgatgg actggccggt cacctccggc atcttcatgt cggcgtccac cttcccggcc
     8161 gacaccagca ggttgatccg gcggcccggc gtgatgcgct cccccgactt ggggctctgg
     8221 tcgatgacga tgttcgccgc gctcttctcc gagggaacat acgaaacgat ccccgtggcc
     8281 accgagatgg tgcggtcgtg cacgtgctgg ttcctgagct tgttcacggc gaagggcagc
     8341 tccatgccca ccagattggg gacggtgaga tggagggcgc tgcggctcac gaggagcttc
     8401 acgcggctcc cctcggcgac gatgctcccg gcggacggat gctggctcag gatgaagccg
     8461 tcatcgacat cgaacacgtc gtagaaacgc acgtcggggc gcaggccctt gttgctcagg
     8521 ctgttgtaga tgtcgccgaa ctgcttgccc accacgtgcg ggaccttgac ctccttgtcg
     8581 ggccgggtga gaaagacgag catgatcgag gagataagaa ggtaaaacga aaacccgata
     8641 aaaaagatga aggcgatatg ccggacggac ttgaagtagc tgaacttcgt ctcgggcgcg
     8701 ccgccggtct gtgtgtcctg tgccattgtc atatccaatt agttttgtag gggcagggct
     8761 ctgccctgag ccaactagaa atttcgcctg gacgattccg tcaagcgaaa tacgtcaccc
     8821 gatgccgtgt cggcggagct tctcgtagag cacgctccgg ctgatgccca ggagcttggc
     8881 cgccttcgac cggttgttgc cggccatcga gatgaccgag cggatgtgcg ccgcctccag
     8941 gcgggccatg gtcgcctcca ggtcgggatc gaagtcctcc gcagcgacgc cctcgctctt
     9001 ctccgggagg atgtgcagaa tggtgccggt ggtgatccgg tcgctgttct cgacgatgag
     9061 cgcgtactcg atcaggtgct cgagctcgcg gatattgccc ttccactcgc ggttacggag
     9121 gatctccagc gcctgctcct cgatgaagtt cactttctta ttgaggtccc cggcgtattt
     9181 cctgatgaag tgcccgatga gaaagggcag gtcctcgaga cgctcgcgga ggggcggaat
     9241 gacaagttcg atgcccgcca gccggtagta caggtcctcg cgaaaggccc cccggcgcat
     9301 ttcctccttt aaattcacgt tggtggcgca gatgatgcgc acgtccaccc gcaccgagcg
     9361 gctggagccg accggtttca cttcgcgctc ctggagaaag cgcaggagct tggcctgtat
     9421 ggtggggctc aagttcccga tctcgtcgag gaacaccgtg ccgccattgg cctcctccag
     9481 gagccccttc ttcgcgcggt cggcgccggt gaacgccccg cggacgtgcc cgaagagctc
     9541 cgactcgatg atggtctccg tgagggtgct gcagtcgatg ggcacgaagg gcttggcatg
     9601 tcgctcgctc tcctcgtgga ccgcgcgggc cagaagctcc ttaccggtgc cgctctcccc
     9661 gaggatgagc acggtcgcat tggcgcgcga aacccgcttc acccgggtga tgagcttctt
     9721 catcaccggg ctctcgccga ccatggacac gccgccgctc aggaccgcgc cctgctcctt
     9781 gatgcgtgcg agctccttct tgagcgcggc gtgctcgagg ttcttctcga ccttgagcct
     9841 gaattcgagg ggctggaagg gcttggatat gaaatcgctc gcgccgttct ttatggcctt
     9901 cacggcgtca tcgacccccc cctggccggt gatgatgatg acgggcatct ccgcgtcgtg
     9961 cgtcctgatt ttcgctagaa gctcgaagcc gtcgagcacc ggcaggaaga gatccgagat
    10021 gaccaggtcg aactcctggc gcatgaagac cgccagcgcc tcgccgccgt cggcgcacgc
    10081 gaccgtggtg tggccgcttt tggttaaaat gtcggcaata tagtcaagaa tcggcggatt
    10141 gtcatcgaca attagaatat ggcccatact ctgtttcgct gtgcggcatg gcgcatgatc
    10201 gccgtgtgcg tttctatgga gaatgcgcgg gatcccggcg ggcgccgcag cgttgcgcgc
    10261 tccccgcgga tagcaatggt gggcgtacgg gcggaaatgg caagcatatt tgttcggggt
    10321 gatgatgaaa cgcgattcgc gcgattgcgg cgcgtcggag gcgctgcgtg attaagatcc
    10381 tgctcaccgc ggacctgcac tgggggacta cgcgcgacgg cctggcgtcc ctcaacgcgt
    10441 gccgggcgaa gaccttcgag cgcatcatgt cgctcgccgc cacgcacgac ctgcttctgg
    10501 tggccggcga tctcttcgac ggcctcggga cggacgatcc ggcctaccgc gcggccgccg
    10561 agcggttcgg cgccgtactc tccggcggta ccgcgatcgc gctgatcccc ggcgagaccg
    10621 agtgcgggga cgacggcgcg ccgcttcccg tcatcgacac cctcccggca agccgcgtct
    10681 tttcgctcac tgacgagggc cccgttcgcc tggagcgcgg cggggagatc gtgcatgtct
    10741 acggcgcacc cgctcgcatg cccttcgatc cggctcgcta tgcccggaca aacgacgacg
    10801 gcttccacat cggcctgttc cacgggaact tcaccgtcaa ggaagacggc gacggggcgc
    10861 ccgtacacac gctcacccgg gaactgattc gtgagtgcaa cctggatttc ttcgccgtgg
    10921 gacacaccca tggctttcgc atcttcaagc agcaaaaccg ggtgatcggc gcctccccgg
    10981 gcagccccga ggcggccgcg cgcaccgaaa cgggcgaacg ctccgttctg tcgatcgcgc
    11041 tcgagaacaa cgagatcacc agcatccggc gcgttatcgt caacacggcc gagctgcggg
    11101 cgctcgagct cgactgcggc gcgtgcgcaa gcgacgatgc gctgttcgac gagctcgcaa
    11161 agaacaagtc gccggtggtc cattgcacgg cgcgcctcgt cggccggcgc gaattcttca
    11221 tcgaccccgc gcgcctggat cagctgcgcc gcgaatttct ctccctcgac atcacggacg
    11281 agtccgcccc cggcctcgga gcgctcctcg gcgagttttc ccgcgaaaac tccctgcgcg
    11341 gcgagttttt ttccctgctt gacgacatgc gccggcggag cgccctcccc gcagacgagg
    11401 acgagacgca cctgcgaagc gtgctcgagt cgctgggccg caccgggacc tatcgcgatg
    11461 aggattggcg atgaagatca cgggcgtcac catcgcgaag ccggggctgt tcgacaatac
    11521 gcggctcgcg ccgggcgagg gcgtgaccgt catctacggc cgcagcgggt ccggaaagtc
    11581 cgccatcgcc cgcgcgctca tcgacgtcct gggcgcgggc atggaggcgg acggctcgcc
    11641 ggcccattcc tgggagaacc tcttcgtcga ggtatcgctc gcctcgacgc acggctcatt
    11701 ccgtttcgtt cgaaacggcg agcgctcctt cgagatcgcc tccgtcgagg gttcgggaga
    11761 gccgcgcgag atcgtccacg acgaccagat cgggcgaaat ccggacgcgg cgcggctgcg
    11821 gatccgcgag gcgctgctcg cgcacgccaa cggccacgca tcgctcgcgc tcctcgcgtc
    11881 gggctcgcgc gcatacctga cgacgatcgc ctacctcgcc gcgccggtgg acacgctcag
    11941 cgcgccggcg ctccacggcg aatcgctgcg ccgcctcctg ctggacgatg cgacgcggtt
    12001 ctacggcctg agccaggcgc tcaacgccgc cttcggcccg tcggcctccg ccgagggaat
    12061 cgaggccgag ctgcgccgcg acgcggtcga gctgcgcgac atggagaagc gcgtcgagct
    12121 catcgacatc caacgcgaga aattcgacaa gctccatcgc gagatggccc tcgtggagcg
    12181 cgagatcgac gagctcgagg gcgcggcctc gcggctgcgc gaccgggtgg ccatgctggg
    12241 cgccgtccgc gatctcctcg aacggcgcga ggtcctgcgc gcccgcatcg ccgatatccg
    12301 cgcgcagctg gacgaggagg cgtccaaccg ggaggccgcg gagcgtctgg ccgccgagat
    12361 agcggacgcc tacccccagt tcgccaattt cgacgaggcc aagaagcgaa acctcggcga
    12421 gatacaggaa cgcttcaccg acatccgcga catcaacgag acaatcgcgg agtatcacga
    12481 cgccctcgcc gcccgcacga aaaagctgaa gatcgtttcc accgcgctca tcgccgcttc
    12541 cttcgcggcg cttgtccttg ccgtcacgcg gaccggcatc gccctggccc cgtccaagat
    12601 catcacgatg ggtgcgatct tgggcgggct ctcggccgta tcgctcgcgg cgctggggct
    12661 gctcgcgacg ctgggaaagc gcacgcgcgc catcaccaca ttgctgaacc gcaaggcggc
    12721 ggccgaggag cggctgcagc gcatcctgaa cgagaacaat cttcccatac gcgacctcac
    12781 gctggcgtcg ctctacgaat acctcctgca atacttcaac gacttcaaca atttcacaga
    12841 gaaacaactc gagctcttca ggatccggga gtcgatgcag gggccggcct gcgcgcgcgt
    12901 gctggagcac gagctcgagg gcgcgctcgc cgacgagcgc gccctcggcg cggagatcga
    12961 cgcggtgacg gcggagctgg gcggtgcggc cgacaacggc tccctgcagg acgcgatcga
    13021 cgcgatcgtc gtcgagaccg cctcgctcga ggaatcgatc aacgaaaaac gcgccgttat
    13081 gcgccaggtg accgacgaga tcctgggcca ggaggaccgc ggggccgagc gggccgcgct
    13141 cctagcggag atcgccgccc gacgggaacg gcacgagcgt catgcgcgcc acgtggaaac
    13201 catgcgcttc gtccgcggcg tgctcgacga ggcggtgcga cgccgggagg agaagcagcg
    13261 ttaccgtctc atcaaccgcg cggcggagcg gttccacacg ctaacggaca accagtacat
    13321 ctccgccatc gaccgcgaac acgtccgcgc cctcgtcgcc ggcgacgccc tccccgcggg
    13381 gacgagccag gtggtcgagc acctgatgct catcgccgtg aagctcgccc tcaccgaatt
    13441 tatgattgac gccggcggcg cgctccccct catacttgac gagccgttcc tgtatatgga
    13501 cgatcgccgg gccgcgcgct tcagggagct cctcgaggcg accgcaccca tgcgccaggt
    13561 gatcctgctg acgcacaaca acctgtataa agactgggga acctacatcg agttatagaa
    13621 tggaccggaa tggccagctc acattttcag acgatccgct ccttccccgc ctcaacgagg
    13681 cctaccggct catcgaggac gggtccttcc acgaggcggt cgcgatcttc gacgagctga
    13741 tggacgcaaa ccccgactat cccggggtgc cggagggcta ccgcgtcgcg cgcttctgga
    13801 acaaccgcgc cgtggagatg acgcaggagg ccgagggcaa aaaaaaagcc gactacctga
    13861 tgcggcagtg gaaggacttc tcgaaatacg caggcgagaa gcagatcgcc gattccgccg
    13921 ccgcgcgcgc ggcgatgaag tacatcttcc aggtcgcgtc gcgccagtac cgcatcgcct
    13981 tccagcgcgg cgagggcgcc gcggacaact tcgagctcct gctcaacctg ggatactgcc
    14041 tggtcacgct gggcgaccac aagaacgtca ttgacaccct cgagtatgcg cgcagctcct
    14101 accgcgcgag cgcgcggctg ctcgcgatcc tgggcgagtc gtacttccac acgggcgatg
    14161 tccccaagag cctgctctac ttccgcgagg ccttctttct cgacccgtcc gagatcgacc
    14221 tgtcgatcgt caaggcgaaa cccgtgaccg atcttctcca aacggtccga acggagcgcc
    14281 cgggctgcgc cgatccccgc gaatgggcgc ccgtctacgg ctatctgcag gacgtcttct
    14341 atgtgcgccg gcagctgggg tcgcaccaga tcgagtccat caaaaaggac atctatacgc
    14401 tggagacgaa cttccagacg ctctcccgcg accgcatcga aaactccaac atcacgccgc
    14461 gcctcatcaa caagtacctc tggatgctcg actacttcga gtatcagaac tacgacttcc
    14521 agagcgtgac cgagattcgc cagcgactcg tcgccatcga caaacggctc ttcgaggaac
    14581 acttcaagcg cgagaagaag aagtagcgtc tctgtagggt ccgggaacgt cctggcacta
    14641 cggagacgcg gcgactccgc aaaattgaat tgacactgcc ctttccggac gcgtaatgac
    14701 gacgaactca tcgcacgcaa ggggcccgtc atggactttc catcaaccta caatcccgcc
    14761 gaggcggaaa cgaaatggta cgagcgctgg gagcgggagc gacgctttca cggcgacgtg
    14821 accgacggca atgagccgta ttccatcgtg atccccccgc ccaacgtgac gggaaacctc
    14881 cacatggggc acgccctcaa caacaccctg caggacattc tgatccgctg gaagcgcatg
    14941 caggggaaat ccgcgatgtg gatgccgggg atggaccacg cgggcatcgc cacgcagaac
    15001 gtggtggagc ggctgctgcg cgaggaggga aagaccaaga acgacttggg ccgcgcggcc
    15061 ttcgagaaac gcgtgtggga gtggaaggag cacagcggcg ggcagataca aaaccagctc
    15121 aagcgcctgg gctgctcgtg cgattgggag cgcgagcgct tcaccctcga cgaggggctc
    15181 tcgcgcgcgg tgcggaaggt tttcgtcacg ctctaccagg aggggctcat ctaccagggc
    15241 gagcgcatca tcaactggtg cccgcgctgc gagacggcgc tctcggacat cgagaccgaa
    15301 taccaggacc tgcagggcaa gctctactat atcaagtatc ccgtaaaggg atcgtccgac
    15361 ttaatcaccg tcgcgaccac gcggcccgag acgatgctcg gcgacaccgg ggtcgcggtc
    15421 aatcccgagg acgagcgcta caagcatctc atcgggacca tgctcattct gccgctcgtg
    15481 aaccgcgaga tcccgatctt cgccgacgcc ttcgtggcgc aggagttcgg cacgggactg
    15541 gtcaaggtga cgccggcgca cgatcccaac gacttcgaga tgggcaagcg ccacgacctg
    15601 cccgagatca acatcctgga taagaacgcg aacatcaacg agaacggcgg gccgtacaag
    15661 gggctcacgc gcttcgaggc ccgcgagaag gtcgtgaagg acctcgacgc gctgggcctg
    15721 ctcgtgaaga tcgagaacca cgaccactcg gtggggcact gctatcgctg cagcaccgtc
    15781 atcgagccgt acatgtccaa gcagtggttc gtgaagataa agccgctggc cgacgaggcg
    15841 attcgcgcgg tggagaacgg cagcatccgc ttcgttccca cgaactggtc caagacctat
    15901 ttcgagtgga tgtacaatat ccgcgactgg tgcatctcgc gccagctctg gtgggggcac
    15961 cgcatcccgg cgttctactg ccgcgactgc ggccacatca ccgtggagat ggaagatccg
    16021 tcacaatgcg cgcggtgcgg atcgacgcgg ctcgcacagg acgaggacgt gctggacacc
    16081 tggttctcct cggccctgtg gcccttctcc acgatgggct ggcccgacat taccgacacg
    16141 ttgaagaaat actacccgac gagcgtgctg gtgaccggct tcgacatcat cttcttctgg
    16201 gtcgcgcgga tgatcatgat gggcctgaag ttcatgaagg acgtcccgtt ccgcgacgta
    16261 tacatccacg cgctggtgcg cgacgagcac ggccacaaga tgtccaaatc gcggggcaac
    16321 gtcatcgatc cgctcatcat gatggagcag ttcggcaccg acgccttccg ctttacgctg
    16381 gccatcttcg cggcgcaggg gcgcgacatc atcatgtccg agaagcgcat cgagggctac
    16441 cgggccttct gcaacaagtt gtggaacgcc tcgcgcttca tcgtcatgaa cctgggtgat
    16501 gatttcgcgc cggcaccgat cgacacgaag gcgctcgagc ccttcgaccg gtggatcctc
    16561 caccgcctga acgacgcgat cgccgacatc acccgcgcgc tcgacgagta caagttcaac
    16621 gaggccgcgc aggcggtgta cgacttcctc tggcgcgagc tctgcgactg gtacctggag
    16681 ctctccaagc agcgcctcta cgcgaaggag gcgggaatgg cggcatcctc ggaaacggcg
    16741 cggcaggtcc tctaccacgt gctcgcgacg gcgctgcggc tcctgcatcc cttcatgccg
    16801 ttcatcaccg aggagctctg gcgcatcatc agcccgcagg gcgagatgct catcaccgcc
    16861 tcgtggcccg cgccggaccc tgcgttcaat ttcgcgtacg aggccgccga gaccgagata
    16921 ttcaaggaga tgatctacaa gatccggaac gtccgcggcg agatgaacat cgccccggac
    16981 aagaagctca acgcggtgtt caaaacggga agcgatgcga tttcgtccat cgtcaaccgg
    17041 gaggccgcga acatccgggc gctcgcgcgg gtgaacgaga ttagcgtgga cgcggcgtac
    17101 gcgccggaca agaccgacgc cgcggccgtc atgaaggacg tcgagatcta cctcccgctc
    17161 acgggcatca tcgacttcgc ggtcgagcgc gcccgtctcg aaaaggagat cgcgcgcgtc
    17221 tccggcgagc tcgagaaggt ggagaagaag ctcggcaatc cctccttcat cgagaaggcg
    17281 cggcccgacg tgatcgagaa ggagaaggcg aagagcgcgg agatggccga cctcctcgcg
    17341 aagctgcggg gaagcctgga gaagctgccg agtcaggact aacaccgacc gccgtgtttg
    17401 tctgatatac cgcgcccgcg acatttccct cggcggcgca ttatgccgtt ttccaataac
    17461 gcgatcaccg tcgcagcggt ggccgatgcg catcggatga tgcggcgcga aacctactcg
    17521 cgcccgtgcc gccgccccgc caaaccctca agccatgcat gtatgtcgcc ggcgacgcgc
    17581 tcccatcccg gctccgaaat gatgaagtgc gcgtgattgt cgtattcccg gtacacggcg
    17641 ttcgcgcgat acttcccgtg tatccttttg acgacgctcg acgggacgag attgtcgtgc
    17701 gcggcgccga tgacaagcag cggaaccgtc atgccgcgcc cgtcgacgcg ggaggccttt
    17761 tcggaatcga aaaccgggaa gccgacctgg agcagggtcc gccccgactc gggcacgatg
    17821 aaggagtgga gcctctcatg catcccgatg ggcatgcggt tgaatacgcc gtatgatgtc
    17881 gcggcggatg agagccggca ggcggatcgc caccatctcg gccggaggac aatgcccgca
    17941 agcgtccgca ggttgcgacg cgtgaacaga aagacgccct gcggcggggc gggcgtgatc
    18001 agtacgccgg cgctgacgcg gacgcgggag cggccgatca ggatctgcgc gatgaggccg
    18061 cccagggagt gccccagcac gattggcggt tcgggaagcc cctggatgag ttgttccatg
    18121 tcatcgacat agtccagaag accggtctcc gacaggcgcg ggtcgggaat ccggtcgggg
    18181 cgcatttcat gatggcgcag cgtgggcacg aggcagcgat aaccgagctt ctcgaaaaac
    18241 ccgcgatacg ccatccagca gatcgcggtg gtcatcatgc cgtggatcat cagaatggtt
    18301 gtgctcatgg cgccctatgt cggaaacaac acatgcgatt acggaaaccg tattgatatt
    18361 ttacatcgcc tacaaacgtg acccctgagg ctctgacccc tgaggctctg acccctgaga
    18421 ctcaccatga ggccaccaca tggcgaaaag tcaaaccgtt tgcatggcgc acgtcgatga
    18481 ccaatccgct ttttttattg acaatcatgc aattggagca ttcactcatc tttccaaaaa
    18541 acaatcggag cgccacccgg tgaagatagg catcgtcatt ctccttttgt gctgcctcgg
    18601 ctgcggatat tcctcggcgg agcgggtcgc ttcgtggcag gcgctcttcg acgatgccag
    18661 cgccgatccc ggcgcaatcg ccacgaggac ggactggacg ccgatcgcgc tcccgtcgat
    18721 gttccggctc ccctacccgc ccgcgcgcac attccgctat ctctggctca ggggcgcggc
    18781 gaacattgcg ggcgatccgt cccagtacgc cggcatccgc ctgggccgca tctactacat
    18841 cgacaggacc tatgtgaacg gcatcctggt gggaagccag ctcaccgaag agtacaacgc
    18901 ggtgcacaat ccccgcgcct acgccatccc gaagggggtc ctcaagaccg ggaaaaacga
    18961 ggtgctcatc cgcctcggca tctacgggac cgagtacggc gggctgctcg acgaggtcct
    19021 gctgctgggc ccgcgcgact tcatcggcgc gcgcatctcc gacgagttca tctaccgcca
    19081 gctccccatc ggcatcgcct tgttcctcct cgggcagatg gccttcaaca tgatcttctt
    19141 catctggcgg aaaaaacaac gggcgaacct ctacgcggcg gcgatgtgcc tgatctggtc
    19201 gctgtacatt ttcgcgctct tctcgcccta cttcccgttc accgacgatt ttcgcataac
    19261 gtttctgtgg agctgcacct cgttcgtccc catcctgttc ctcatgctca tccagtcctt
    19321 ctacaaggtg tacctctcct acttcaaccg ggtggtcatc ccgctgctgc tcgccgtcac
    19381 cgggatcgtg ctggcgtttc aggacaccac cgccgcgttc tatcccggcc ccattctggg
    19441 gaccggcacc ctgttcgccg ttatccccct cctgtcgtat gtgatgtacc gggtcaactc
    19501 ccgccggccc gaccccatga tcttcgtctt catcggtttc ggcctgctgc cgggactgtt
    19561 catcggctgg gacatcgtca actacctctg ggtccatcac tatccgccgc tcacgcacac
    19621 ctacaccatc ccggtgttca tcgtgggggt gatgattctc atcatccgcg agttcatcaa
    19681 gaaggagatc gagctggacc tcctgtatcg aaaactcgag gagccgtcgt ccgaggagcc
    19741 ggtcaagaag cccggcatca cctcctcaac ggaggggaag ctcgagaaga tcatcgagtt
    19801 cctgaaggag aattacgcgt cggacatatc gcgcgagggg ctggcggggg cgatgaacct
    19861 ctcccccgac cacatgagcc gcatgttcaa ggcctacacc ggcaaaaaga tcaacgacta
    19921 catcaacgag ctgcgcataa ccgacgcggc ggggcggttg gccgatcccg aggtgaagat
    19981 catcgacatc gccttcgccg tgggcttcga gagcctggcc accttcaacc gctcgtttct
    20041 caaggtcacc gggaaaacgc cgaccgattt ccgggccgcc ctgaagaagg acgactgatt
    20101 tattattgac atccgtcttt gcgcgctcga tactctgaca gacgccgttc gcatcacgat
    20161 cgttcgacac ctgcggcctg gagcacccca tggaaaccgg cacccttgac agaaagctgc
    20221 ttctcggatt tcccctgcgc gtctttctaa gcacggacct gatcacctat ctcgtcagca
    20281 tccccgtgac ggtgttcatc tcctacctgg tcctccccat ggaggggacc cagcggatcg
    20341 tcttcttctc ggtcgtgggc gccgtcgtgc tcatcgccgt caacctcacg tttttccaaa
    20401 cgagggcgct cttcgccccc gtcacaaagt atttcacgaa gcttctggac gatgaaccga
    20461 cgacggatgc ggagtacgtc gcggcgtgga actgtttctt caccgccgcg cggcggcgcg
    20521 cgatcgagtc gatcatcgcc tggacggtcc tcatgccggt cgcgatcatc gtgttcacgg
    20581 ttttcttcca cccgacgctc acctcgcgca tcgtgatcgg attcctcctg ctcgccaaca
    20641 tcatcttcgc cggcgtctac tattatctgg tgatcgacag ccaggtccgg aagatcgccc
    20701 ggctggggat tttctcccgc cggatggagg gatgcaacat cgcgcggcgg ccgctcagcc
    20761 ttaccatcgc catcatcatg ctcggcatgg tcgcattctt ctgcgcggtg gtcatcccgg
    20821 tcatcttcgc catcaccaac tcgttccagg agcgcgcgta cctgagcgag atgaagatcg
    20881 tgatcacgct gatcgacgcc gatctcgaaa aacgcttcga cgagcgtcgc gcgccccgcg
    20941 gcgccatcgc cccggacggc gcggacccga tcgcccatat ccaggacctc gtgaagcgga
    21001 ctcgcgtggg cgagaccgga tacccgtttt tcctcgacga ccgcaacgcc gtcatcggcc
    21061 accccgaccc gaagcagatc ggccagaaca tcgaccggtt cgacttcggc cgaaggctca
    21121 tcgccctgcc cagcggctcc catttccgct acctctggca ggacaggtat aaaatcatgt
    21181 acttcgtgaa gaacaagaag tacggcttca cttcggccat caccgcgtat ctgttcgaca
    21241 tcgagcggca cagcctgtat gtggtcgcga tcatgtgcct cttcctggtc gccggattca
    21301 tctgcaccgg cctgtgctcg tactggttcg tcgccacgcg gctcaggccg ctctcccggt
    21361 gccgacagct catcgacgag atggggaacg gcaatctgag cgtggaattc accggcgtga
    21421 caaacgacga gatcggcatt atcatgaaca cgctcaacga cttcatctcc cggctcaggg
    21481 acgttatccg caacatccag ggaatcgccc acgagatggc gagctcggcc gaggagatgt
    21541 cgtcggccgc gctctcgttc tcggagaacg cgcagaacca ggccgcctcg gccgaggagg
    21601 gatcggcgac ctccgaggag gtcgcctcgg gcgtggagca catcgcggac ggcgccgagc
    21661 ggcagctggc gagcctgaac gcgctcatcg gagagatcaa cgagctcgcc ggcagcatca
    21721 ccgaaatggg cgagcggatc gaacagaccg ttcacatcaa cgaggacatc tcgcgcaagg
    21781 cgacttccgg cgacgaggcg ttgcgcacga tgaacgcctc catgcagcgc atcaccgaca
    21841 gctcgcatga gatgaccaac atcgtcaaga tcatcagcga catctccgag cagatcaacc
    21901 tgctctcgct caatgcggcg atcgaggcgg cgcgggcagg cgaggcgggg cgcgggttcg
    21961 cggtcgtggc cgacgagatc tccaagctcg ccgaccaaac ggcctcaagc atcaaggaga
    22021 tcgacaagta catcaagagc agcaacgacg aggtggggcg cgggatggcg aacgccagct
    22081 ccaccagcca gctgatcatg tccatcatcc agggggtcgc gcagatcagc ggcatgatgg
    22141 cggagctcgc cgatcggatg cggcggcaga cggccatcaa ccgcagcgtg accgagaacg
    22201 ccgagacggt gcgcaaccgc gccgaggaga tctcgcttgc gaccgacgag cagaagcgcg
    22261 cgatggacga aatcgttcgg acgatcacgc acatcaacga gctcacgcag gcgaacgcct
    22321 ccggcgccga ggagatggcg ggaaacaccg agggtctggc cagcatggcc gaggcgctca
    22381 aggcgtcgat cgatttcttc agggcctccg agtagacggg cgattatttc ctgatcacgt
    22441 gcaccgcggt cgctttgaag gacgccgcga cctgccggcc cggcgcgagc cgcagccgct
    22501 ccagcgaatc cgccgtcacg tgtgagacga ggggaaagcc gcaatcgagc cgcaccttga
    22561 agaacactcc cttccgatac acgtcgacaa cgcgcgcggg aaatgagttg cggacgctcg
    22621 agcgcgcgcc agcgcgcgcc ggacccaccg aaagcgagac attctccggt cggatgcaca
    22681 gcgcgaccgc gctcccatgc tccgccgcaa cggcggtttc gatcgcgtgc cccgacacgt
    22741 ccacgaccgc cgcgccgccg cgcgtgctcg cgacgcgccc caccagcacc gtttccatcc
    22801 cggcgaatga cgccatgaac tcgctcacgg gattgttgaa catgtcgtcc gcgggtccat
    22861 cggcgacaat gcgcccgcgc tccatcaccg cgatgcggtc cgtgagcgcg agcgcctcct
    22921 gtgcatcgtg ggtgatgatg acggcggtcg cgcccgtttc ccgcagggtg cccgcgaggt
    22981 cggcgacgag cgattcgcgc gcgttcgcat cgagcgacga gaagggctcg tcgagaaaga
    23041 tgagatcggg cgcggtcgcg aaggcgcgcg cgaggctcgt ccgctgcgcc tcgccgcccg
    23101 agagcttctg tgcggcgcgg ccgcgcaggt ggtcgattct cagacgatgg agcgtctcat
    23161 cgacccgccg cgcgatctcg gcgcgcccca tgccgcgcat gcgaaggcca gaagcaacgt
    23221 tatccgcaac gctgccgctg aacaacagcg gctcctggaa cacgaaggcg catcgtcggc
    23281 ggtaggccag acggtcgcgc cgcgttgcga ctggcgcgcc ccggaacgag atcgttcccg
    23341 tcgtggttcc gagcaatgtg gccagcgtaa gcaggaggct cgtcttgccg ctgccgtttg
    23401 ggccgaccaa tcccagcgtc tctccggcgg cgacggcgag agaggaaaca tcgagcacgc
    23461 gaacgccgcc gcggtgaacg gtgacgccgg aaagttcgat gagcggcgtt accggtcccg
    23521 tcatcgccgc ttctcccgct gctgtatgat ggtcaggacg ccggtgatca ggaatgccag
    23581 aagaagcaaa atgatgccca gcgcgatggc gagctcgaag ttccccttgc cggtctccat
    23641 caccgtcgcg gtggtgagca cccgcgtgaa gccctttata ttgcccccca ccatgatgga
    23701 ggcgcccacc tcggagatca cgccgccgaa gccggccatg acggccgcaa ggagcggcaa
    23761 ccgcgcctct ttcatgagga tccagaccat ctggatccgc gtcgcgccca gcgagagtat
    23821 ctgcagccgg aggttgggcg gcaggttctg catggccgcg atggtgatcc cggtgacgat
    23881 gggcaccgcg atcaccgctt gcgcgatcac catggcgacc ggcgtgtaga ggatctccag
    23941 gaaaccaaag gggccgtttc gccacaggaa catggtcacg aacaggccca ccacgaccgg
    24001 cggcaggccc atgcctgtgt tgataaggct gatgaccacg cgtttcccgg gaaattcggc
    24061 cagcgcgacc gccgcgcccg acgagatgcc cagcagcatg ctgatgagcg tcgccgaccc
    24121 ggagatctgc aatgagagca gcgttatgcg cagcacctcc ggatcgagcg tcaccagtag
    24181 atagaacgcc tgccagatgc cgtcgagaaa aatgcccatt tacttgccca gcgactcgac
    24241 cctcttgccg gcgtcgggaa agaagagcgg cgagccgaat ttctccacgc caaaggtctt
    24301 gatgatcttc tgggtttcct tggcgaccat gaaatcagcg aatgccgtgc cgcccgcgac
    24361 gttcaccttg ggaaagtttt tcccgatgag ttcgatgacg tggtagatat tcaagagttt
    24421 cgcgtcgccc tcgaccagga tgtcgagccc gagtttcttc ttgagcgcca aatacgtgcc
    24481 ccggtcggca agagtgtacc ccttcttctc ggccgcgacg gccagcgtct gacccatgcc
    24541 caggccggtc tgctggtacc acttctggcc ctcggccttg acggcggccg ctttccagag
    24601 gttcttctct ttcgcgtgcg tgccggagtt gtccccgcgg gacacgaaga gcgactcgga
    24661 cttcgcgatc ttcttcaggg catcggcgga ctttttggtt cccttgatct tcgcggggtc
    24721 ggcggccggg cccacgataa tgaagtcatt gtgcatcacc agccggcgac gcgcgccgaa
    24781 accggctttc atgaaggcct tctcgtcgtc gggtgaatgg accaacagca cgtcggcttc
    24841 gcccttcctg cccatcgcca tcgcctggcc ggagcccacg gcgatcgtct tcaccatgaa
    24901 accggtcttc ttctcgaaga tggggatgag cacatcgagc agccccgtgt cctgtgtgct
    24961 ggtcgtcgtc gcgaggatga cgcttttcac cttctgcgcg gcggcggggg aagcgcccca
    25021 tgaaccggcg agcagcgcca tcgctacaat caaaaatgcg gttatcttcc tgttgcagtc
    25081 catatagata ctcccactgt aatgatgaaa tttttcctga atgatccaaa agcgactact
    25141 ttattacacc gtaaagctcc aatatcatac aataaacaac aatatttttc agccatatga
    25201 tttttccttt ggcagtgcga ttcactcccc atttccgaga aaaattatac aagcattcat
    25261 gcacagtatg ccactgcgcg agaaaattca ccgacaacga gctgtgtcac aaccactaga
    25321 aaaaacattt gactccgact aatcgagtac ccacgctggc aaaatattca ttattttaac
    25381 caataataca ataatctaac ttatacctac gggaatcgga catgttcgag ggaaaaacgc
    25441 tgtggaacat catgcagatg ggg
//
LOCUS       DAORHF010000030        24903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1268_length_24903_cov_6.167579, whole genome shotgun sequence.
ACCESSION   DAORHF010000030 DAORHF010000000
VERSION     DAORHF010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24903
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_24903_cov_6.167579"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PKJ16_04430"
     CDS             complement(<1..1220)
                     /locus_tag="PKJ16_04430"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNV46267.1"
                     /translation="MGESVTYKILREHLVDGTLDPGSEIGIRIDQTLTQDATGTMAYL
                     QFEAMGIPRVKTELSVSYVDHNTLQMGFENADDHRYLQTMNANFGIHYSRAGNGICHQ
                     VHLERFGRPGATLLGSDSHTPTGGGIGMIAIGAGGLDVAVAMGGGPFYLACHRVVNIM
                     LTGSLRPWVSAKDVILTVLGILTTKGNVGTVVEYTGPGVATLSVPERATITNMGAELG
                     VTTSVFPSDEVTRAFLKAQGREAAWIELVPDSDAVYHRTIEIDLASVEPMVACPHSPD
                     AIKRAAELRGMKITQVAIGSCTNASYKDLATVAKILAGKKAHPDVSLIVAPGSRQVFE
                     MIARDGSLADLIASGARIMESACGFCIGSGQAPVTEGVSLRTNNRNFEGRSGTESAQV
                     YLVSPELAALSAIAG"
     gene            complement(1223..1636)
                     /locus_tag="PKJ16_04435"
     CDS             complement(1223..1636)
                     /locus_tag="PKJ16_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV46268.1"
                     /translation="MTAPGVPSSGNPLVDGVNAQLALVTVEEYKLGAPVSAQERADMI
                     DRAVAVIKAMLPRLPEGYVIQVTGHTDTQGGAQSKANLAVSARKAQLVFGELGKKGVA
                     GARLVTKGAGGTLLSTACGEKEPCQRRVTFIVVKK"
     gene            complement(1823..2461)
                     /locus_tag="PKJ16_04440"
     CDS             complement(1823..2461)
                     /locus_tag="PKJ16_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNV46269.1"
                     /translation="MEKNYLRIVWILCIITIAYNIVEGIVSTFFGAGDDTIALFGFGL
                     DSFVEVISGVGVAHMVWRIRRAGDLAQRDGFERRALRITGAAFYLLAGGLVCGSALTI
                     YRGGRPETTVVGIVIALISIATMWALYAFKVRYGRLLGSEAVLADAGCTKTCLYLSFI
                     LLASSLLYEMFKIGYFDVAGGLGIAWYSIREGREAFQKSRNDTRTCGCADCH"
     gene            complement(2527..3612)
                     /locus_tag="PKJ16_04445"
     CDS             complement(2527..3612)
                     /locus_tag="PKJ16_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46270.1"
                     /translation="MPDEPTPFSRPMRTVLWIVMSLAFALGFLAANREDMFERLHIFL
                     FNLCCCGFVILRDVARHAATADHSVPFLALSLAYAGCAALGFYLPAAVLALALAALVE
                     SVRARAFSPLPLDIFSLRTDVAEKFRHAAVLCLSIALALSALVIINNEYGRWIAMRKL
                     TLTMFFLGFSFPMSLMTMALVVDGLRPSFRGSTRRILEAVFWIITVGVILFLAAILLE
                     FAPAEIALATMLFTAVIALLALFAHGAERSPRAAYCASGIAFLVSTGISGITYIFVNR
                     YGSCATCGDVVLWSHAYLSLYGWNLCGLALIVHAPSDAPRALPARVLIPLHWLSVALA
                     PFGKLYPQAAVVAVIVFAVIVFALVKK"
     gene            3702..4349
                     /locus_tag="PKJ16_04450"
     CDS             3702..4349
                     /locus_tag="PKJ16_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46271.1"
                     /translation="MRCAIIIPPPFTAACRRGLAIASAVVFIAGCAAAPATTRLPKPD
                     LAAAQHDRSELIIALGDDNVKVLEALRFLQEKEPDTYNALFDYIRNIMPGSHPYWSRI
                     RYPDTIEISTYTFTRGRVYLASLLFHELCHLMFIRIRLEPEHPVENERLIAILRRYDP
                     FITVEEIRGLSFFEVERKIHFIQLRFLYRYGDIENAKAVKDAIGELPKTYRHLLP"
     gene            4475..5578
                     /locus_tag="PKJ16_04455"
     CDS             4475..5578
                     /locus_tag="PKJ16_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV46272.1"
                     /translation="MSTKDLVSIIVPTFREAKNIPVLTERIHEAMKAARIAYEIIVVD
                     DNSQDGIAEAVDALRKKFPISIKVRLTERGLSSAVIEGFRLAKGTVFAVMDADLSHPP
                     EKIPELVAPIMGGSAEFVVGSRFVPGGSAEHFNWFRRLNAWVSKTLARPFTTVRDPMA
                     GFFAFPKSILPDLTLLNPLGFKIGLEVIVKGNPRTTMEIPIQFKERLYGESKLSIKEQ
                     VNYLRHIIRLFEYKYESLAEMIKFGFVGGTGTVVNMAFVFLSYDILSISYRIAVAIGF
                     LVSATSNFILNRTFTFPKSRQLHSAVQYALFIVVGGAGFAVNWFIAVYLYENNPFFHE
                     HYLLATFLGILGGMTINFTGSKLLVFRKGTTSA"
     gene            complement(5565..6782)
                     /locus_tag="PKJ16_04460"
     CDS             complement(5565..6782)
                     /locus_tag="PKJ16_04460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV46273.1"
                     /translation="MTVDFMRTVDRYVGIPACLLLSFVHRLNPFTARTKPSSVKKILF
                     LQISEMGSAISAYSALRKAQAMFPDAELYYFIFSEMQESVHLLNIIPRERVLTVTSKS
                     FFGFIGGLLRMIVTIRRMRFDAILDLELFSRLSSLLSFLFGARYRAGFYRFHMEGLYR
                     GTMQTHRVVYNHTRHISENFLALVYALAEAPGREPLSKVCIDENDIVRPRIPITPAGR
                     RHIGDLLRRVCPSITPRHRIVLFNPNASELLPLRRWPMENYIALAKRLVRDARVIVAV
                     TGAPSEARDADAIVAGVASERCVSLAGKTTLRELVDLYHCSHVLLSNDSGPPNFASLT
                     PIRVLVFFGPETPLCYKPLGNQIEALYSSFACSPCVSAYNHRKSACTENRCLQAITVD
                     EVYRRVTGALKRS"
     gene            complement(6789..8507)
                     /locus_tag="PKJ16_04465"
     CDS             complement(6789..8507)
                     /locus_tag="PKJ16_04465"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HNV46274.1"
                     /translation="MNDTTSLPRILRNAADIFALIGALVLFSGGLYFHDAIFGSVVVA
                     RISEFGIFFIPLLLLGIFAPDIARQTRAAVALRSVHRWMLALPARRQTALYGAIIGIT
                     GALHIAGIIRKYLSFRMDRDLAIQANACANYLMASHKGDTHLLADHFEPFLMLFSPLC
                     ARFDPSVVVLVGCTLFWIAGALGIYRIARSERCSFPVSLAFSWLYLVFWGHVNVVYFD
                     SHLLALAVGFLPWIVWSVRTDRFVLFCILAILYMGLKENAALTIAGFGMLMILTRRYV
                     KGAIVVAAGFAAFVVIMTVVYPHFTKGEGTVYFAKYYGHIGGTMKEFLLSCLTRPLYV
                     LSTVITVPKMIYLLRLFAPFAFIHVLRPVYLVPIIPSIMINILSNWDRLISMDFNYES
                     EIFPYLFATSVILAYDPAVRARLYALSRLPARWLRMPFVRPGLSARVVVITVVFVFFS
                     GSSPAFHYSFNRPEQFQIHLRMMLPKLIDRADRVAAIEKVAPHLSHLRHIYYLNEWEK
                     SDTIVIAYPDFQRLWQYGFEEIETKIVPQFDEKYELWYRDARYPLFRVWKRKASAPLE
                     IPPASR"
     gene            complement(8510..9568)
                     /locus_tag="PKJ16_04470"
     CDS             complement(8510..9568)
                     /locus_tag="PKJ16_04470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV46275.1"
                     /translation="MTPAAWKKILAIQIKQMGDVLLTTPAVRCLRRAFPDAQIDFAVQ
                     SNIAVIVEDNPDINNLVIVNPKSGFFDYVSFLRSVRRTGYDAVFDFIGTPRSAVIARV
                     SGAPVRYGFDFDGRKFAYTHVVQRDRSPKYEVDYKFDLLRGAGIACAELDLVLGVNDA
                     ARARAAEIFRELGLDAPGARGKGVILLSPISRRQARRWHITSYARLASLAADAGFVCV
                     AVTGPGEEQMGEEIVRGSGGRALMLPPTSVKEMAACFALADATVANDNGPMHISMAVG
                     TPTIAIFGPTKPESWMEPGGRHRWLKGNVPCIGCEKQECNEMICMTDVTPEAVLAVVR
                     EVCAGQTETTAPESNRTR"
     gene            complement(9585..10685)
                     /locus_tag="PKJ16_04475"
     CDS             complement(9585..10685)
                     /locus_tag="PKJ16_04475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV46276.1"
                     /translation="MDIKYPVMRPYIDEAEKRLVAQSIDDGWISSIGPYVERFERLFA
                     EYAGAPYALSCSNGTTALHLALMAIDLAPGDEVIVPDITFIATANAVTYAGATSVLVD
                     VEPDTWAMDPKEIEKKITKRTKAIIPVHLYGHPADMDPIMAIAKANGIAVVEDAAESL
                     GARYKGRFSGCIGDIGCFSFYGNKIITTGEGGMVVTASEELYKKMQLLRNHGMTKERR
                     YWHPVIGYNYRMTSLQAAFGCGQMEKVDAIVKKRVADAAVYHRELAGVPGIVPHPCRE
                     WAYNVYWMYSILVEDPTRRDGLMQALLEDGIETRPFFYPVHEMPRYERPNEYPVSTRL
                     ARAGINLPTYFAMTEEDIVYICGRIKTHVQRA"
     gene            complement(10705..11871)
                     /locus_tag="PKJ16_04480"
     CDS             complement(10705..11871)
                     /locus_tag="PKJ16_04480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV46277.1"
                     /translation="MPRGVHNKKTVLMVNGHYPPAKCGMGDYTKRLADEVRATGAYDV
                     DVLTAAYPGIAKIRTERGVRLLRIPRRWDAAEYGKLREHLRGRYDVLHIQYHDGDYPS
                     LELLRRLPRLMKDDGRVGSVMISMAGFKDSDRSLEKLFAESDLVNVTTDEDGARIRRI
                     TGIAESKLRTIYDGPNMLPMNERYIHTPRTQVGAKKKPARLLHFGFLSPQKGFEEIVE
                     ALRIVSDSGVEFSLDVIGSDHEARETDYFKRIKTLIKKKGMGERITFHGFIGGAKMNR
                     IFSQADLAIFPFRDGISGKRSSLWSVMSFGVPCISTESAMVPAGIRHGENILLTPARE
                     PRVLASAVIAAVSDDALRARLSRGALEVVRRQYSWERIRTEVVDAYEAMLHGRL"
     gene            complement(11968..12543)
                     /locus_tag="PKJ16_04485"
     CDS             complement(11968..12543)
                     /locus_tag="PKJ16_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HNV46278.1"
                     /translation="MKSVKGTRTEKNLLASFAGESQARNRYTYFASVAKKEGYEQISA
                     IFLETAENEKEHAKKFFKFLEGGMVEITAAYPAGVIGTTVENLKASADGENEEHTMLY
                     PEFAKIAEEEGFKEIALLWRNIASVEKHHEERYRALAKNIQDQKVFKKDAPVAWKCRN
                     CGFVIEGKDAPEKCPTCDHPKAYFEMLDDNF"
     gene            complement(12754..13131)
                     /locus_tag="PKJ16_04490"
     CDS             complement(12754..13131)
                     /locus_tag="PKJ16_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HNV46279.1"
                     /translation="MTKKLEVYKCEVCGNMVEVVHEGAGQLVCCGQPMKNLVENTTDA
                     AKEKHVPAIEKAAHGIVVKVGSVAHPMTPEHFIEWIQVIFKSGRTTRVFLNPGQAPEA
                     AFPVDEDYESVREYCNLHGLWKA"
     gene            complement(13209..14207)
                     /gene="cydB"
                     /locus_tag="PKJ16_04495"
     CDS             complement(13209..14207)
                     /gene="cydB"
                     /locus_tag="PKJ16_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013171509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HNV46280.1"
                     /translation="METIDILRHAWFVLIGVLFVGYSILDGFDLGIGALFPFLARDDS
                     DKRALVNSIWPVWDGNEVWLLTAGGAMFAVFPQAYATVFSGFYLALMLVLFALIFRAV
                     SLEFWYYDEARRTLWQWAFTIGSLLPALLFGVALGNVVIGVPLDAAMEYAGDFFTLLR
                     PYPLLIGLLGLSAILLQGATYAVLKTDGALRERARAATRATWIGFIVMFNAALIATIV
                     YQPLFFRKTLALLFVFAVVAAWLALAVAQRRRRDGWAFLFSSLVFAGLWGVAGASHHP
                     FLVKGGGDALTSITIANGSSSELTLTVTLVIALIGMPVVIGYTAYVYRVFRGTARG"
     gene            complement(14239..15564)
                     /locus_tag="PKJ16_04500"
     CDS             complement(14239..15564)
                     /locus_tag="PKJ16_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HNV46281.1"
                     /translation="MDAVLLSRIQFALAAGFHFLFPPITLGLALIIVILETKYLRGGD
                     ELVKRMSAFLVKILALVFTVGVATGIVLEFSFGTNWSNYSRFVGDIFGAPLAAEGILS
                     FFLESVFLGVLVFGRTRVSKRAYWLSAVLVCFASHLSGLWIIIANSWMQTPAGFRIEN
                     GRAVMTNFLEVVINHSSLVRYLHVVIASWITGSLFVAGIAAWNIVTARHEEVFRPLLK
                     LSLAVFAAAVIIQLGSGHSHSVQVAKTQPEKMAAFEALWETTAGAPLSLFGIPVEKER
                     KTYLEVSVPKLLSLLVHFNPNEKIAGLSEFPDNEKPPVLLTYASYHVMISLGFLFPAI
                     AGVGFLLFVRKRLFDTPWYLWALVFAVPLPHVANLFGWMAAEVGRQPWVVYRVLRTAD
                     AASVVVPAGQVLFTLIMFGLIYALLFVVFLVILAKIIRTGPDGAAHEAH"
     gene            complement(15599..15757)
                     /locus_tag="PKJ16_04505"
     CDS             complement(15599..15757)
                     /locus_tag="PKJ16_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HNV46282.1"
                     /translation="MKKYKCSVCGYVYDPSEGDPDNGIKPGTKFEDLPDSWVCPVCGV
                     GKDQFEEA"
     gene            complement(15846..16268)
                     /locus_tag="PKJ16_04510"
     CDS             complement(15846..16268)
                     /locus_tag="PKJ16_04510"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HNV46283.1"
                     /translation="MMKNDVVQLLEEKHIKPSLHRIKILEYLMVHRNHPNVDMIYKAI
                     GKEIPTLSKTTIYNTLKIFQESGIVQFLTVEENEVRFDATMQHHAHFRCVTCGAVHDI
                     ELSSKVFEMESVDGHRITGSQIYFFGHCRDCSQLRVIS"
     gene            complement(16383..17543)
                     /locus_tag="PKJ16_04515"
     CDS             complement(16383..17543)
                     /locus_tag="PKJ16_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV46284.1"
                     /translation="MRYVIIGNGVAGVEAALAIRKADADGTITIITKSPNLHYYRPRL
                     IEFLSGEVSLDKMTTYKDDAYEKKGIANLLGTRITAIRPDERSAIDANGRAHEYDRLL
                     IATGGRPFVPPIAGADLPGVFTFRGVRDAERIRDYAAGVRDVAVIGGGLLGLETAHSL
                     ARMGKRPTVVEFCEWLMPRQLDRDGGGILRRILEEKGVRFVLGESVASIEGGGRAERI
                     VMQSGATVDAGLIVISAGIRSDLDLAREVGIATDKGIIVDDTMRTSAEGIYAAGDVAQ
                     HKDCVYGIWPAAREQGIAAGAAMAGQPVAYRGTVMSSSLKITGVDLYSAGDINRDDCD
                     AIVSSGGSSYKKLLMKDRKATGAIVLGDAAALKIAQRVIAGTADPEEFKKLF"
     gene            complement(17540..18700)
                     /locus_tag="PKJ16_04520"
     CDS             complement(17540..18700)
                     /locus_tag="PKJ16_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HNV46285.1"
                     /translation="MNPIKILDDVYWVGGIDWNLRNFHGYLTQRGSTYNAYLIVDDAV
                     TLIDTVKHYLTDEMLDRIAWIVDPSEIKYIVANHVEMDHSGALPRLMEICPDATIITS
                     PQGEKGLRAHYRKDWNFKVVQSGEEVSIGARTLKFVLTPMVHWPDNMVCYSPSDKILF
                     SNDAFGQHIATSERFDDQFYVDIIHEEAMKYYANIVLPYSQQVRKVLEDAATLDIEYI
                     APSHGIIWRSYVSWILEFYQRWAMNETDNKALIIYDTMWGSTEKIAYAIQKAFEIREI
                     DTSMLLLKNNHMSDIMTEVLTARYICVGSPTLNKNMLPDVAAFLTYLKGLAPRNKVGL
                     AFGSYGWGAQGVNDVDAVLRACDFDMIDKISTQYVPDAKALDEITKKVMEAL"
     gene            18825..19859
                     /locus_tag="PKJ16_04525"
     CDS             18825..19859
                     /locus_tag="PKJ16_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46286.1"
                     /translation="MMMTRRSLIAGAFAAIAGLAPPVVLSAVRQSSGADTIPEASYLR
                     LYRSGELKQRGERLRGMMARCRLCPRECGANRLAGARGFCGATAEPMVAAHYPHFGEE
                     RPLVGRHGSGAVFLSNCSLRCVFCINADISQGGAGDTVTVARLASMMLELQSKGCRNI
                     NVVTPTHYSAHILLALDAAARRGLRIPLVYNTCGWERPEVLALLDGVVDIYLPDMKYW
                     DPERAARYSAGARSYPELSRAAIAEMHRQVGVARPAADGSLYRGLIIRHLVMPNRVGG
                     TREILAWIARTLPKDTYVNLMSQYRPVHRAREFHEIARGITRAEYAEAVRWAREAGLT
                     RVDLQREPGA"
     gene            complement(19996..20658)
                     /locus_tag="PKJ16_04530"
     CDS             complement(19996..20658)
                     /locus_tag="PKJ16_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV46287.1"
                     /translation="MRHMRAVSAMRFWAAWTAGLAVWAIGLIMIAPHDLTIAQAVYNP
                     QSIPARAVALAGEWPLWIVFLGSLGALAFHARLFTDRPRYRDVATLIVLQAIVHPFLI
                     TQALKRLWGRVRFRDLSPGLLDYTHFYEPAGPGAGQSFPSGHVAMSLILAPLVFYVFA
                     SQKRAAALALLGAVSAYGLFVSYCRMVTGAHYPTDVAFSFGCSFLVAALIVRYAPMGP
                     DR"
     gene            20870..22174
                     /locus_tag="PKJ16_04535"
     CDS             20870..22174
                     /locus_tag="PKJ16_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46288.1"
                     /translation="MRLLVPGANTRMIDIGKLYCGGASTGDGLRYGTESGADCHGNRP
                     HRVVVSARERRPIVVWSVTRTCNLNCVHCYTDSANRRYADELSTDEGLRLIDDLAAFG
                     IPSLLFSGGEPLMRRDLFTLIERASARDIRPVISTNGTLIDRDAARAIKDSGIVYVGI
                     SLDGMEDVNDEFRGQKGAFSKAMKGFENCVAVGQRVGLRLTLTRHTYADLHRIFDFIE
                     REGINRACFYHLVYSGRGSGIFADDLTHGQSRDAMDIILARTKDFFERKRDINILTVD
                     NHADGVYLYLKLRESDPHRAEEVRELLAWNGGGAHSTGVGIGNIDFVGNVHPDQFWQD
                     YSFGNIRERSFGDIWTDESESLMKGLKNKADYIKGRCRLCAHRALCTGAMRVRAYRVY
                     HDPWAPDPQCYLTDEEIGLTDEMKRELAARGESFPMPPELAR"
     gene            22178..22447
                     /locus_tag="PKJ16_04540"
     CDS             22178..22447
                     /locus_tag="PKJ16_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46289.1"
                     /translation="MAVSEQCAICGAALTEPHSNYPRAVCPACDARALNAQGEPARHI
                     TQTVPAEEAHRYAFTDDGDNPVFIDGIRCTRRYKFGGWVSMRDDQ"
     gene            22482..23135
                     /locus_tag="PKJ16_04545"
     CDS             22482..23135
                     /locus_tag="PKJ16_04545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HNV46290.1"
                     /translation="MKPYPAFLNLEGRSVVIVGGGGVALRKVKDLLDAGARITLVAPS
                     VLPEIAAIAAAEPARVAIRERAYEPGDCAGAALVISATDDGEVNRAVFREAEERNLFI
                     NAVDDPENCSFIVPSTIRRGDLLIAVSTGGLSPSMAARLRRELERHVPEDIEVKLEAL
                     AAARKALRENPRLSHLTSAERGEVLKYIANNDEVLNKLITATIAGEVVELLLTIVGR"
     gene            23986..24573
                     /locus_tag="PKJ16_04550"
     CDS             23986..24573
                     /locus_tag="PKJ16_04550"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46291.1"
                     /translation="MNTRPERKDEIIAASLALIERKGIQNLTTKNISREVGITEPGIY
                     RHFKNKQEILTEILRRFKNGIGERMIIAKERATNPLDQIGALYEGSLRMFNEYPSIAA
                     AIFSEEIFQNNDRLSSEVRSIMELSQSGLRDMIRRGQSEGCIRNDQPDEQLSLIVMGA
                     FRLLVTRWRLMKRSFDIIADGRKLWKTMRALLAVK"
     gene            24620..>24903
                     /locus_tag="PKJ16_04555"
     CDS             24620..>24903
                     /locus_tag="PKJ16_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV46292.1"
                     /translation="MNTLVRFFINRPMLINIIILAVIGLGVKSVMEARKEGFPEISMN
                     KIIIQTIYPGASARDVELNVTVPIEDALEELEGIKEVLSVSEEGVSRIEV"
BASE COUNT         4945 a         7835 c         7630 g         4493 t
ORIGIN      
        1 ccggcgatgg ccgaaagcgc cgcaagctcg ggactcacca ggtacacctg cgccgattcc
       61 gttcccgatc gcccctcgaa gttccggtta ttggtacgca gcgacacgcc ctcagtgacc
      121 ggcgcctgcc cggagccgat gcagaagccg caggcggact ccatgatgcg cgcgccgctc
      181 gcgatgaggt ccgcgagcga cccgtcgcgg gcgatcatct cgaagacctg ccgcgatccc
      241 ggcgcgacga tgaggctcac gtccgggtgc gcctttttcc cggcgaggat cttcgcgacg
      301 gtcgcgaggt ccttatagga tgcgttggtg caactcccaa tcgcgacctg ggtgatcttc
      361 atgcctcgca gctcagcggc gcgcttgatc gcgtccggcg agtgcgggca tgccaccatc
      421 ggctccaccg aagcaagatc gatctcgatg gtgcgatgat agaccgcgtc cgaatcgggc
      481 acgagctcga tccaggctgc ttcgcggcct tgtgccttca gaaatgcgcg cgtcacctcg
      541 tcgctgggaa acacggacgt ggtaacgccc agctccgcgc ccatattagt gatagtagcg
      601 cgctcgggta cggagagcgt tgcgacgccc ggcccggtgt attccacgac cgtccccaca
      661 tttcccttgg tggtgaggat gccgagcacg gtaaggatga cgtccttggc cgatacccac
      721 gggcgaagcg aacccgtaag catgatgttc accacgcggt gacaggcgag atagaacggc
      781 ccgccgccca tggcgaccgc gacgtccaac ccgcccgcac cgatcgcgat catgccgatg
      841 ccgccgccgg tgggcgtgtg gctgtcggat cccagcagcg tggcgccggg gcgaccgaag
      901 cgctccagat gcacctggtg gcagatcccg ttgccggcgc gcgagtaatg gatcccgaaa
      961 tttgcgttca tggtctgcag gtagcggtga tcgtcggcgt tctcgaagcc catctgcagg
     1021 gtgttgtgat cgacatagga gacggacagc tccgtcttca cgcgggggat ccccatcgcc
     1081 tcgaactgaa ggtaggccat ggtgccggtc gcgtcctgcg taagcgtctg gtcgatgcga
     1141 atgccgatct cgctgccggg atcgagcgtc ccgtccacga ggtgctcgcg gaggatcttg
     1201 taggtgacgg attcgcccat cgctattttt tcaccacgat gaacgtgacg cgccgctggc
     1261 agggctcctt ctcgccgcaa gcggtcgaca gcagcgtacc gcccgcgccc ttggtgacga
     1321 gccgcgcacc cgcgacgccc ttcttgccga gctcgccgaa caccagctgc gccttcctcg
     1381 cgctcaccgc gagattcgcc ttggattgcg cgccgccctg cgtatcggtg tggccggtga
     1441 cctggatcac gtatccctcg gggaggcggg gcagcatcgc cttgatgacg gcgacggcgc
     1501 gatcgatcat atcggcccgc tcctgcgcgc tcaccggcgc gcccagcttg tactcctcga
     1561 cggtgaccag cgcgagctgc gcgttcacgc cgtcgaccag cggattgccc gaggacggga
     1621 ctccgggagc cgtcatcggc gcgggctttc cggcgcatcc ggcgcacgcg agaacgagaa
     1681 cgaacgcact aataaaaata tttctactgg gtgtaatcat catacggctc ctttgttgaa
     1741 atgatagcgt cacgcccgaa cgcataacgg attgtaggcg atcacgggaa aaataatgca
     1801 agaaaaaaac aggcgatccc tgtcaatggc agtcggcgca accacaggta cgcgtgtcgt
     1861 ttcgtgattt ctgaaacgcc tcgcgacctt cacgaatcga ataccaggcg attcccagcc
     1921 ctcccgcgac atcgaaatag ccgatcttga acatctcgta taaaaggctc gatgccagca
     1981 ggatgaacga gagatacagg caggtctttg tgcagcccgc gtccgcaagg accgcctccg
     2041 atcccagaag ccggccatac cggaccttga aagcgtacag cgcccacatc gtcgcgatcg
     2101 atatcagggc gatgacgatc ccgacgaccg tcgtttccgg ccgtcctccc cgatagatgg
     2161 tcagcgcgct tccacaaaca agtcctcccg ccaacagata aaaggccgct ccggtgatgc
     2221 gcaacgctcg gcgctcaaaa ccgtcgcgct gcgccaggtc gcccgcgcga cgtatccgcc
     2281 ataccatgtg cgccacgccc acgcccgaaa tcacctcgac gaaactgtcg aggccgaatc
     2341 cgaacagcgc aatggtatcg tccccggcgc caaagaaggt ggagacgata ccctcgacga
     2401 tattatacgc aatggtaatg atgcaaagaa tccatacgat gcgaagatag ttcttttcca
     2461 ttctttccgt ttcccgcaca gatcgtgctc ccgtagagat aattataccg cactgcgacg
     2521 cggttttcat tttttcacaa gcgcgaacac gatcactgcg aacacgatca ccgcaacgac
     2581 ggcggcctgc ggatacagct tcccgaacgg ggcgagcgca acggacagcc agtgcagggg
     2641 gatcaggacc cgtgcgggaa gcgcgcgcgg cgcatcagaa ggggcgtgca cgatgagcgc
     2701 gagaccgcag agattccatc catacagcga cagatacgca tgcgaccaca gaacgacatc
     2761 gccgcatgtc gcgcacgagc cgtagcgatt gacgaaaatg taggtgatgc cgctgatccc
     2821 ggtggacacc agaaacgcaa tgccggaggc gcaatacgcc gcgcgcggcg atcgctccgc
     2881 cccatgcgca aagagcgcga ggagcgcaat caccgccgtg aagagcattg tcgcgagcgc
     2941 gatctccgcg ggcgcgaact ccaacaggat ggcggcgaga aacaggatca cgcccaccgt
     3001 gatgatccag aatacggcct cgaggatccg tcgcgtcgat ccgcgaaacg acggacgcag
     3061 gccgtccacg accagcgcca tcgtcatgag cgacatgggg aatgagaaac caagaaaaaa
     3121 catggtgagc gtgagcttcc gcatggcgat ccagcgcccg tattcgttgt tgatgatgac
     3181 cagcgccgag agcgcaaggg ctatggagag gcacagcacc gccgcgtgcc ggaacttctc
     3241 cgcgacgtcc gtccgaagcg agaagatgtc cagggggagc ggcgaaaacg cgcgcgcccg
     3301 caccgactcg acgagggccg cgagcgccag cgccaggacc gccgcgggaa gatagaaccc
     3361 caacgcggcg caccccgcgt acgcgaggct gagcgcgaga aacggcaccg aatgatccgc
     3421 ggtagcggcg tgacgggcga catcgcgcag tatgacgaag ccgcagcagc agagattaaa
     3481 gaggaagatg tgcagccgct cgaacatgtc ttcgcggttg gccgccagaa agccgagtgc
     3541 gaatgcgaga ctcatgacga tccagaggac cgtccgcatg ggccgtgaga agggggtggg
     3601 ttcgtccggc atacgcgcat ctccgagcgg ggcggaatca acgatcaatg gggacagcat
     3661 atcgtgcttg cttttttttg caatcgggat acggttcttc catgcgatgc gccatcatca
     3721 tcccgccgcc gtttacagcc gcctgccgcc gggggcttgc gatcgcgtcc gcggttgttt
     3781 tcatcgcggg gtgcgccgcc gcgcccgcaa ccacgcgcct tcccaagccc gacctcgctg
     3841 ccgcgcaaca cgaccggagc gagctcatca tcgccctggg ggacgacaac gtcaaggtgc
     3901 tggaggcgct gcgctttttg caggaaaagg aacccgacac ctacaacgcc ctcttcgact
     3961 atatccgtaa catcatgccg ggaagccatc cctactggag ccgcatccgc tacccggaca
     4021 ccatcgagat atcgacctat accttcacgc gcgggagggt gtatctcgcg tcgctgctct
     4081 ttcacgagct gtgccatctc atgttcatac gcatccgcct ggagcccgaa catcccgtgg
     4141 agaacgaacg cctcatcgcc atcctgcggc gatatgatcc gttcatcacg gtcgaggaga
     4201 ttcgcggcct ctccttcttc gaggtcgaga ggaagatcca tttcatccag ctgcgcttcc
     4261 tctaccgcta cggcgacatc gaaaacgcga aggccgtcaa ggacgcgatc ggcgagctcc
     4321 cgaagaccta tcgccacctg ctgccgtaaa gaccgacggc gcgagcaggg ccttttcatg
     4381 atctcgaacg gacccgcggc ccgccgatgg ataatacttg attaaaaaca gaccctctgt
     4441 ttctctggcg gtaaacgatc acaggcggcc atccatgagc acaaaagacc tcgtgtcgat
     4501 tatcgtcccg accttccgcg aagcgaaaaa catccccgtc ctcaccgagc gcatccatga
     4561 ggccatgaaa gcggcccgga tcgcctatga gataatcgtc gtcgacgaca actcacagga
     4621 cggcatcgcg gaggcggtgg acgcgttacg aaagaaattc cccatcagca tcaaggtgcg
     4681 cctcaccgag cgcgggctct cgtcggcggt catcgagggg ttccggctcg ccaaggggac
     4741 ggtgttcgcc gtcatggacg ccgacctcag ccatccgccg gagaagatcc cggagctggt
     4801 cgcgcccatc atgggcggca gcgccgagtt cgtggtcggg agccgtttcg tgccgggcgg
     4861 ctcggcggag cacttcaact ggtttcgccg cctcaacgcc tgggtgtcca aaacgctcgc
     4921 gcggcccttc accaccgtgc gcgaccccat ggcgggcttc ttcgccttcc cgaaatcgat
     4981 cctcccggac ctcacgctcc tcaatcccct gggattcaag atcggcctgg aggttatagt
     5041 caaggggaat ccccggacaa cgatggagat ccccatccag ttcaaggagc gcctgtacgg
     5101 cgagagcaag ctttccatca aggagcaggt gaactatctc cggcatatca tacgcctgtt
     5161 cgagtacaag tacgagagcc tcgccgagat gatcaagttc gggttcgtgg gcggcaccgg
     5221 caccgtcgtg aacatggcgt tcgtgttttt atcctacgat atcctgtcga tctcctatcg
     5281 catcgccgtc gcgatcgggt ttctggtgtc ggcgacatcg aactttattt taaatcgcac
     5341 cttcaccttc ccgaagtcgc ggcagctcca ttcggcggtg cagtatgcgc tctttatcgt
     5401 cgtcggcggg gcggggttcg cggtcaactg gttcatcgcc gtgtatctgt acgagaacaa
     5461 tcccttcttc catgaacatt acctgctcgc gaccttcctg ggcatcctgg gggggatgac
     5521 gatcaacttc accgggagca agctcctcgt ctttcggaag gggactacga gcgcttgagc
     5581 gcgcccgtga cgcgccggta gacctcgtcg accgtgatgg cctgaaggca gcggttttcg
     5641 gtgcaggccg acttccgatg attgtaggcc gacacgcagg ggctgcaggc gaaactagaa
     5701 tagagcgcct cgatctggtt ccccagcggc ttgtagcaca acggggtctc cggcccgaaa
     5761 aacaccaaaa cgcggatcgg cgtgagcgac gcgaaattcg gcgggcccga gtcattgctg
     5821 agcagcacgt ggctgcagtg gtagaggtcc acgagctcgc gcagcgtcgt cttgcccgcc
     5881 agcgaaacgc agcgctcgct cgcgacgccg gcgacgatgg catcggcgtc cctcgcctcc
     5941 gacggcgcgc ccgtcaccgc aacaatcacc cgtgcgtccc ggacgagccg tttcgccagc
     6001 gcgatatagt tctccatggg ccagcggcga aggggcagaa gctcgctcgc gttcggattg
     6061 aacagtacga ttcggtgccg cggcgttatc gacgggcaca cccggcgcag cagatccccg
     6121 atgtgcctac gccccgcggg cgtgatggga atcctcggcc ggacgatgtc attttcatcg
     6181 atgcagacct tcgacagcgg ctccctgccc ggcgcctccg cgagcgcata gaccaaggcg
     6241 agaaagtttt cgctgatatg ccgggtatga ttgtacacca ctcggtgcgt ctgcatcgtc
     6301 ccgcggtaca ggccctccat gtggaaccgg tagaaccccg cccggtaccg cgcaccgaac
     6361 aggaagctca gcaggctgct gagccgcgaa aagagttcca ggtcgaggat cgcgtcgaag
     6421 cgcatccgcc gaatcgtcac gatcatgcgc aggagcccgc cgatgaagcc gaaaaacgat
     6481 ttcgaggtga ccgtgaggac ccgctcccgc ggtatgatgt tcagcaggtg cacgctctcc
     6541 tgcatctcgc tgaaaatgaa gtaatacagt tcggcgtcgg ggaacatcgc ctgcgccttt
     6601 cgcagcgcgc tgtaggcgga gatcgccgat cccatctcgg agatctgcag gaacaggatt
     6661 tttttcaccg acgacggttt cgtccgcgcc gtgaacgggt tcaggcgatg cacgaatgaa
     6721 agaagcaggc acgccgggat gccgacgtat cggtcaacgg tccgcatgaa atcgacggtc
     6781 atagttcgct acctcgaagc cggtggtatc tcgagcggag cggaggcctt ccgtttccac
     6841 acgcgaaaga gcggatagcg cgcatcgcgg taccagagct cgtacttctc gtcgaactgc
     6901 gggacgatct tcgtctcgat ctcctcgaag ccgtactgcc acaggcgctg aaagtccgga
     6961 taggcgatga cgattgtgtc ggacttctcc cactcgttaa gataatagat atgccgcaga
     7021 tggctcaggt gcggcgccac cttctcgatg gccgcgaccc ggtcggcgcg atcgataagc
     7081 ttcggtaaca tcatcctcag atggatctga aactgttcgg gccggttgaa cgagtaatgg
     7141 aaggccggcg acgagccgct gaagaaaacg aacacgacgg tgatgacgac gacacgcgcc
     7201 gatagccccg gccgaacgaa cggcatgcgg agccatcgcg ccggcaaccg cgatagcgca
     7261 tacagtcgcg cgcgcaccgc cggatcatac gcgagaatga cggaggtcgc gaacaggtat
     7321 gggaatatct ccgactcgta attgaagtcc atgctgatga gccggtccca attggacagg
     7381 atgttgatca tgatcgaggg gatgatcggg accagataga ccgggcgtag cacgtgtatg
     7441 aaagcgaacg gagcgaacag gcgaagcagg taaatcatct tggggaccgt tatcacggtc
     7501 gagaggacat agagcgggcg cgtgaggcag gagagcagaa actccttcat cgtgccgccg
     7561 atgtggccgt agtacttggc gaaatacaca gtcccctctc ccttggtaaa gtgcggatac
     7621 accaccgtca tgatgacgac aaatgccgcg aagcccgccg cgacgacgat cgcgcccttc
     7681 acgtaacggc gcgtcaggat catcagcatg ccgaacccgg cgatggtcag ggcggcgttt
     7741 tccttcaatc ccatgtacag gatagccaga atgcaaaaaa gaacgaagcg gtccgtccgc
     7801 accgaccaga cgatccacgg caaaaacccc acggccagcg cgagcaaatg cgaatcgaaa
     7861 tacaccacat tcacatggcc ccagaagacg agatagagcc acgagaaggc gagcgacacg
     7921 ggaaagctgc agcgctcgga gcgcgcgatg cggtaaatcc cgagggcgcc cgcgatccag
     7981 aacagcgtgc acccgacgag gacgaccacc gacggatcga agcgcgcgca cagcggcgag
     8041 aagagcatca ggaacggctc gaagtggtcg gccaggaggt gcgtgtcgcc cttgtgtgac
     8101 gccatgagat aattggcgca ggcgttcgcc tggatggcga gatcgcggtc catccggaag
     8161 gagagatact tgcggatgat tccggcgata tggagcgcgc cagttatgcc gatgatcgca
     8221 ccgtagagcg ccgtctggcg gcgtgccggg agcgcgagca tccagcggtg gaccgagcgc
     8281 aacgcaaccg cggcgcgagt ctgccgcgcg atatccggcg cgaagatgcc cagcaggagt
     8341 aacgggatga agaaaatgcc gaactcgctg atgcgggcga cgaccacgga gccgaagatg
     8401 gcatcgtgaa aatacaggcc cccggaaaac agcaccagcg cgccgatcaa cgcgaagatg
     8461 tcggcggcgt ttctgaggat tcgcggtaat gatgtcgtgt cgttcatatt cagcgagtac
     8521 gattggattc cggcgcggtc gtttccgttt gcccggcaca gacctcacgc accaccgcca
     8581 gcaccgcctc gggtgtcacg tcggtcatgc agatcatctc attgcattcc tgcttctcgc
     8641 agccgatgca gggcacgttt cccttgagcc agcggtgtcg accgcccggc tccatccacg
     8701 attccggctt ggtggggcca aagatggcga tcgtgggcgt gcccacggcc atgctgatgt
     8761 gcatgggacc gttgtcgttg gcgacggtcg cgtcggccag ggcgaagcag gccgccatct
     8821 ccttgaccga ggtgggcggc agcatcagcg cccggccccc cgaaccgcgc acgatctcct
     8881 cgcccatctg ctcttcaccc gggcccgtca cggccacgca cacgaagccc gcgtcggcgg
     8941 ccagcgaggc gaggcgcgca tacgacgtta tgtgccatct tctcgcctga cgccggctga
     9001 tgggcgacag gaggatcacc cccttccccc gcgcccccgg cgcgtccagc ccgagttcac
     9061 ggaagatctc cgccgcccgc gcgcgcgcgg cgtcgttcac gccgaggacc aggtcgagct
     9121 ccgcgcacgc gatcccggcg ccccggagga ggtcgaactt gtagtcgacc tcgtacttgg
     9181 ggctgcggtc gcgctggacc acgtgcgtat aggcgaactt gcgcccgtcg aaatcgaaac
     9241 cgtagcgcac gggcgcgccg ctcacgcgcg cgatcaccgc cgagcgcggc gtgccgataa
     9301 aatcgaacac ggcgtcgtac ccggtccggc gaacagatcg caggaacgaa acatagtcga
     9361 agaaccccga tttcggattg acgatgacga gattattaat atccggattg tcctccacga
     9421 tcacggcgat gttcgactgc acggcgaaat cgatctgcgc gtcggggaag gcgcggcgca
     9481 ggcagcgcac cgccggcgtg gtgaggagca cgtcgcccat ctgcttgatc tggatcgcga
     9541 ggatcttctt ccaggccgct ggcgtcatcg acacgctctc cgtatcatgc tcgctgcaca
     9601 tgggtcttga tgcggccgca gatgtatacg atgtcctctt ccgtcatagc gaaataggtc
     9661 gggaggttga tcccggctcg cgcgagccgc gtggacaccg ggtattcgtt cgggcgctca
     9721 tagcgcggca tctcgtggac cgggtaaaag aacggtcgcg tctcgatgcc gtcctcgagc
     9781 agcgcctgca tgagcccgtc gcgccgcgtc gggtcctcca cgaggatcga atacatccag
     9841 tagacgttat acgcccactc ccggcaggga tgcgggacga tgccgggaac gccggcgagc
     9901 tctcgatggt agaccgccgc gtcggccacg cgcttcttca cgatggcgtc caccttctcc
     9961 atctgcccgc agccgaaggc ggcctgcagg ctcgtcatgc ggtagttgta gccgatcacc
    10021 gggtgccagt agcgccgctc cttggtcatg ccgtgattgc gtaacagctg catcttcttg
    10081 tagagctcct ccgacgcggt caccaccatc ccgccctcgc cggtggtgat gatcttgttg
    10141 ccgtagaagc tgaagcagcc gatgtcgccg atgcagccgc tgaagcggcc cttgtagcgc
    10201 gccccgagcg actccgccgc gtcctcgacc acggcgatcc cgttggcctt cgcgatcgcc
    10261 atgatcgggt ccatgtcggc cggatgcccg tacaggtgca cggggatgat ggcctttgtc
    10321 cgcttcgtga tcttcttttc gatttccttc gggtccatgg cccaggtgtc gggctccacg
    10381 tccacgagca ccgaggtcgc gccggcatag gtgacggcgt tcgcggtggc gatgaaggtg
    10441 atgtcgggga cgatcacctc gtcgcccggc gcgaggtcga tggccatgag cgccaggtgc
    10501 agcgccgtgg tgccgttgga gcaggagagc gcatagggcg cgcccgcgta ctcggcgaag
    10561 agccgctcga agcgctcgac gtaggggccg atggacgaaa tccatccgtc gtcgatgctc
    10621 tgggcgacca ggcgcttttc cgcctcgtcg atatagggcc gcatgaccgg gtatttgatg
    10681 tccattgatt gttccgttat ccgtttatag gcggccgtgc agcatggcct cgtaggcgtc
    10741 cacgacctcg gtgcggatgc gttcccagga gtactgccgt cgcaccacct cgagcgcgcc
    10801 ccgggacagc cgcgcgcgaa gcgcgtcatc ggacacggcg gcaataacgg cgctcgcgag
    10861 tacccgcggt tcgcgcgcgg gcgtcagcag aatgttttct ccgtggcgga tgccggcggg
    10921 gaccatcgcg ctctccgtgc tgatgcaggg cacaccgaac gacatgacgg accacaggct
    10981 gctccgcttc ccggaaatcc cgtcgcggaa ggggaagatg gcgaggtcgg cctgtgagaa
    11041 gatgcggttc atcttcgcgc cgccgatgaa gccgtgaaac gtgatccgct cgcccatgcc
    11101 cttctttttg atgagcgtct tgattcgctt gaagtagtcg gtctcccgcg cttcgtgatc
    11161 ggacccgatg acgtcgaggc taaactccac ccccgaatcg ctcacgatcc gcagcgcctc
    11221 gacgatctcc tcgaagccct tctgcggcga gaggaaaccg aagtgcagga gtcgcgccgg
    11281 ttttttcttc gcgccgacct gcgttcgcgg cgtatggatg tagcgctcgt tcatcggcag
    11341 catgttcggg ccgtcgtaga tggtgcgcag cttcgattcg gcaatcccgg tgatccgcct
    11401 gatgcgcgcg ccgtcctcgt ccgtggtcac gttcacgagg tcggattcgg cgaagagctt
    11461 ttccagcgag cggtcggagt ccttgaatcc ggccatcgat atcatgacgg acccgacgcg
    11521 tccgtcgtcc ttcatgagcc gcggcaggcg gcgcaggagc tcgagcgagg gatagtcccc
    11581 gtcgtggtac tggatatgca ggacatcgta ccggccgcgg aggtgctcgc ggagcttccc
    11641 gtattccgcc gcgtcccatc ggcgcggaat ccgcaggagg cgcaccccgc gctcggtgcg
    11701 gatcttcgcg atgcccggat acgccgcggt caacacatcg acgtcgtatg cgccggtggc
    11761 gcggacttcg tcggcgagac gcttggtgta gtcgcccatg ccgcacttgg cgggcgggta
    11821 gtgcccgttc accatcaaca ccgttttttt attgtgaaca ccgcgcggca tagtatggaa
    11881 tccgttggtc cgataaaaag agtaaccccc tggcgatcca gagggtttct cactcacttc
    11941 cgttggctga aaaaatgcaa gaaaaaatca gaaattatcg tccagcatct cgaagtacgc
    12001 cttcgggtga tcgcacgtgg ggcacttctc cggcgcgtcc ttcccctcga tgacgaaccc
    12061 gcagttccgg catttccacg ccaccggcgc gtccttcttg aagaccttct ggtcctgaat
    12121 gttcttcgcg agcgcgcgat agcgctcctc gtggtgtttc tccacggagg cgatattgcg
    12181 ccacaggagc gcgatctcct tgaagccctc ctcctcggcg atcttcgcga actccggata
    12241 gagcatcgtg tgctcctcgt tctcgccgtc ggcggacgcc ttcaggttct cgaccgtggt
    12301 gccgatgact cccgcggggt aggccgcggt gatctccacc atgcccccct cgaggaactt
    12361 gaagaatttc ttggcgtgct ccttctcgtt ctcggccgtt tcgaggaaga tggcggaaat
    12421 ctgctcgtag ccctcctttt tcgcgaccga cgcgaaatag gtgtagcgat tgcgcgcctg
    12481 cgactcgccc gcgaacgagg cgagcaggtt cttttcggtt ctggttcctt ttacgctttt
    12541 cattcatttc tcctaaatgg gttatacgta ttcctagtgc cccacggccg ctgtgcgata
    12601 gcgatgcgtc cgatccctgg gggctgtgtg ccgcacacaa ccggcggcga acgcgcgatg
    12661 acatccgccg gtcttcgtca gtcgcgcacg atcagcaacc gtcgttcatc gcgcagatcg
    12721 cgcgggcgcc atcaccgcgc gatccgtggg cgtttacgcc ttccacagcc cgtgcagatt
    12781 gcaatactcg cgcacggatt cgtagtcctc gtcgaccggg aaggccgcct cgggcgcctg
    12841 tcccggattg aggaacacgc gcgtagtccg cccgctcttg aagatcacct ggatccattc
    12901 gataaagtgc tcgggcgtca tcggatgggc gaccgacccc accttcacga cgatcccgtg
    12961 cgcggccttc tcgattgcgg gaacgtgctt ttccttcgcg gcgtcggtgg tgttctccac
    13021 caggttcttc atgggctgcc cgcagcacac cagctgcccc gcgccctcgt ggacaacctc
    13081 gaccatgttg ccgcacacct cgcatttata cacttcgagt ttcttggtca tacattcctc
    13141 cgtttggttt tatatatgca taacgccgcc aacacgcgat cgcccgaccg cgtccggcgg
    13201 cggcatcatc agccccgcgc cgttccccgg aagacccggt acacgtacgc cgtatacccg
    13261 atcaccaccg gcatgccgat gagcgcgatg accagcgtca ccgtgagcgt gagctcgctc
    13321 gacgatccgt tcgcgatggt aatgctggtg agcgcatcac cgccgccctt gacgagaaac
    13381 gggtgatgcg acgcgccggc gacgccccac aggccggcga agacgagcga cgagaacaga
    13441 aaggcccatc cgtcgcgacg tcgccgctgc gcgacggcga gggcgagcca cgccgcgacg
    13501 accgcgaata caaagaggag tgcgagcgtc ttgcggaaaa agagcggctg atatacgatc
    13561 gtcgcgatca gcgccgcgtt gaacatcacg atgaacccga tccaggtcgc ccgcgtcgcg
    13621 gcgcgcgcgc gctcccgcag cgcgccatcg gtcttgagca cggcataggt cgcgccctgc
    13681 agcaggatgg ccgaaagccc caggagcccg atcaggagcg gataggggcg cagcagcgtg
    13741 aagaaatcgc ccgcatactc catggccgcg tcgagcggga cgccgatcac cacattgccg
    13801 agcgccacgc cgaacaggag cgcggggagg aggctcccga tcgtgaaggc ccactgccag
    13861 agcgttcggc gcgcctcgtc gtaataccag aactcgagcg agaccgcgcg aaagatgagc
    13921 gcgaagagca ccagcatgag cgccaggtaa aacccgctga acacggtcgc gtaggcctgc
    13981 gggaataccg cgaacatcgc cccgcccgcc gtgaggagcc acacctcgtt accgtcccag
    14041 acgggccaga tggaattgac gagcgcccgt ttgtccgaat cgtcacgggc gagaaacggg
    14101 aacagcgcgc cgatgcccag gtcgaagccg tcgaggatcg agtagcccac aaagagcacg
    14161 ccaatgagca cgaaccacgc gtgtcgcagt atgtcgattg tttccattta tcgttccccc
    14221 ggccgcatgg ataatccgtc agtgcgcctc atgcgccgcg ccatcgggcc cggtgcgtat
    14281 gatcttcgcg aggatgacga gaaacaccac gaacagcagc gcgtagatga ggccgaacat
    14341 gatcagcgtg aagaggacct ggccggccgg gaccacgacc gacgcggcgt cggcggtgcg
    14401 cagcacccgg tagacgaccc agggctgccg tcccacctcg gcggccatcc agccgaagag
    14461 gttcgcgacg tgcgggagcg ggacggcgaa cacgagcgcc cacagatacc agggcgtatc
    14521 gaagagccgc tttcgcacga acagcagaaa tcccacgccc gcgatcgccg gaaagaggaa
    14581 gcccagcgat atcatcacat gataggaggc gtaggtgagg agcaccggcg gtttttcatt
    14641 gtcgggaaat tcactgagtc ccgcgatctt ctcgttcggg ttgaagtgca ccagcaggga
    14701 gagcagcttc ggaacgctca cctcgagata ggtcttccgc tccttctcga cgggaatgcc
    14761 gaagagcgac aggggcgcgc ccgccgtggt ctcccacagg gcctcgaagg cggccatctt
    14821 ctccggttgc gtttttgcga cctgcaccga gtgcgaatgg ccggaaccga gctgaatgat
    14881 caccgcggcg gcgaagacgg cgagcgacag cttcagcagc ggccggaaca cctcctcatg
    14941 gcgcgccgtg acgatgttcc aggcggcgat gcccgccacg aagagcgatc cggtgatcca
    15001 gctggcgatc accacatgca ggtagcgcac cagcgacgag tgattgatca cgacctcgag
    15061 aaaattcgtc atgaccgccc ggccgttctc gatgcgaaag cccgcagggg tctgcatcca
    15121 cgaattggcg atgatgatcc agaggcccga gaggtgcgag gcgaaacaca ccagcaccgc
    15181 cgagagccag taggcgcgct tcgacacgcg agtgcgcccg aagacgagca cgcccaggaa
    15241 caccgactcc aggaaaaagg aaaggatgcc ctcggccgcc agcggcgcgc cgaagatatc
    15301 gcccacgaag cgcgagtagt tggaccagtt ggtcccgaag gaaaactcca gcacgatgcc
    15361 cgtggccacg cccacggtga acaccagcgc gaggatcttc accagaaacg cggacatccg
    15421 cttcacgagc tcgtcgcccc cccgcagata cttcgtttcc agaatgacga tgatcagggc
    15481 gagcccgagc gtgatcggcg ggaagagaaa gtgaaatccc gcggcgagag caaactggat
    15541 cctggacagc aatacggcat ccatacgcta ctcctgtatg tcggcccgaa cgtcgggtct
    15601 aggcctcttc gaactggtcc ttccccacac cgcacaccgg gcagacccag ctgtcgggaa
    15661 ggtcttcaaa tttggttccg ggcttgatcc cgttgtcggg atcgccctcg ctgggatcat
    15721 acacataacc gcacaccgag catttgtatt tcttcattgg aatctcctta aatgtattag
    15781 atacacaagt gaaatgaata cagatttatc aactattttt tctctttctc gatactttta
    15841 cgcagttacg agatgactcg caactgggaa cagtcccggc agtgcccaaa aaaatatatc
    15901 tggctgccgg ttatccgatg cccgtccacc gattccatct cgaacacctt cgaggagagt
    15961 tcaatgtcgt gcaccgcgcc gcacgtaacg caccggaaat gcgcatggtg ctgcatggtc
    16021 gcatcgaagc gcacctcgtt ttcctccacc gtgaggaact ggacgatccc gctctcctga
    16081 aagatcttca gggtgttgta gatggtcgtc ttggacaggg tggggatctc cttcccgatc
    16141 gccttgtaga tcatatccac gttgggatga ttgcggtgca ccatcagata ctccagtatc
    16201 ttgatgcgat ggagcgaggg cttgatgtgt ttctcttcga ggagctgtac cacgtcattc
    16261 ttcatcatgg cgtcaccgat gcacggagcg ttcgcccggc cgggggcccg agggaccgaa
    16321 cggcgcgccg gacgcgcgat tgtatgttga ttgcaaaaac cgtatcacgg atcggcacgc
    16381 tttcaaaata atttcttaaa ttcctcgggg tccgccgtgc cggcgatgac ccgctgcgcg
    16441 atcttcaggg cggcggcgtc accgagcacg atggcccccg tcgccttccg gtccttcatg
    16501 aggagctttt tataggatga tccgccgctc gagacgatgg cgtcgcaatc gtcccggttg
    16561 atgtcgccag ccgaatacag atcgacgccg gtgatcttga gcgagctgga catgacggtc
    16621 ccgcggtagg cgaccggctg acccgccatg gcggcgccgg cggcgatccc ctgctcgcgg
    16681 gccgcgggcc agatcccgta cacgcagtct ttgtgctgcg cgacgtcgcc ggccgcatag
    16741 atcccttcgg cgctggtgcg catcgtgtcg tcgacgataa tgcccttgtc cgtcgcgatg
    16801 cccacctcgc gcgcgaggtc gaggtcgctc cggatgcccg ccgatatcac gatcaaaccg
    16861 gcgtcgacgg tcgcgccgga ctgcatgacg atccgctccg cgcgcccgcc gccttctatt
    16921 gatgcgacgc tctcgcccag gacgaagcgc acgcccttct cctcgagaat gcgccggagg
    16981 atgcccccgc cgtcccggtc gagctgccgc ggcatgagcc attcgcaaaa ctccacgacc
    17041 gtcgggcgct ttcccatccg cgcgaggctg tgcgcggttt ccagccccag cagacccccg
    17101 ccgatcaccg ccacgtcgcg cacgcccgcg gcgtagtcgc gaatgcgctc ggcgtcgcgc
    17161 accccgcgga aggtgaatac gccgggaagg tcggcccccg cgatgggcgg cacgaacggc
    17221 cgcccgccgg tcgcgatgag caaccggtcg tattcatggg ccctgccgtt cgcgtcgatc
    17281 gcactgcgct cgtcggggcg gatcgccgtg atgcgcgtcc cgaggagatt cgcgatgccc
    17341 ttcttctcgt aggcgtcgtc cttgtacgtg gtcatcttgt ccaacgacac ctcgccggag
    17401 aggaactcga tgaggcgcgg acgatagtaa tgaaggttgg gggacttggt gatgatggtg
    17461 atggtcccgt ccgcgtcggc ctttcggatc gcgagcgccg cctccacccc ggcgacgccg
    17521 ttgccaatga taacgtatct cacagcgcct ccatcacctt cttcgtaatc tcgtcgagcg
    17581 ccttcgcgtc ggggacgtat tgcgtcgaga tcttgtctat catgtcgaaa tcgcaggcgc
    17641 gcaggaccgc gtcgacgtca ttgaccccct gcgcccccca gccgtaggag ccgaaggcaa
    17701 ggcccacctt gttgcggggc gcgagcccct tgaggtaggt gagaaacgcc gccacgtcgg
    17761 gcagcatgtt cttgttgagc gtgggcgacc ccacgcagat atagcgcgcg gtgagcacct
    17821 cggtcatgat gtcggacata tggttgttct tgagcagcag catgctcgtg tcgatctcgc
    17881 ggatctcgaa cgccttctgg atcgcgtagg cgatcttttc ggtcgatccc cacatggtgt
    17941 cgtagatgat gagcgccttg ttgtcggttt cgttcatggc ccagcgctgg tagaactcga
    18001 ggatccagct cacgtagctg cgccagatga tgccgtggct gggcgcgatg tactcgatgt
    18061 cgagcgtcgc ggcgtcctcc agcaccttgc gcacctgctg cgagtacggc agcacgatat
    18121 tggcgtagta cttcatcgcc tcctcgtgga tgatatcgac gtagaactgg tcgtcgaagc
    18181 gctccgaggt tgcgatgtgc tgtccgaagg cgtcgttcga aaagaggatc ttgtccgacg
    18241 gcgaatagca gaccatgttg tcgggccagt gcaccatggg cgtgaggacg aacttcagcg
    18301 tccgcgcgcc gatggacacc tcctcgccgg actgcaccac cttgaagttc cagtccttcc
    18361 gatagtgcgc gcgcagcccc ttctcgccct gcggcgaggt gatgatggtc gcgtcggggc
    18421 agatctccat gagccgcggc agcgcgccgc tgtggtccat ctccacgtgg ttggcgacga
    18481 tgtacttgat ctcggagggg tccacgatcc atgcgatgcg gtcgagcatc tcgtcggtga
    18541 ggtaatgctt cacggtgtcg atcagggtca ccgcgtcgtc gacgatgaga taggcgttgt
    18601 aggtggagcc gcgctgcgtg agatagccgt ggaaattgcg caggttccag tcgataccgc
    18661 ccacccagta gacatcgtcg aggatcttga tcgggttcat ccacgcctcc aaggaccgct
    18721 gatgtatcac tctacaatag cgcatcgcgt atcggcaata aaaaaattta tccgcacccg
    18781 ggccgcggct tgacccgacg cgcgtcgggg tgtatcttac ccgcatgatg atgacgagac
    18841 gatccctcat cgccggcgcc ttcgccgcga tcgcgggcct cgcgcccccc gtcgtgctat
    18901 ccgccgtgcg acagtccagc ggcgcggaca cgatccccga ggcctcgtac ctgcggctgt
    18961 atcgctccgg cgaattgaaa cagcgggggg aacggctcag ggggatgatg gcgcgctgcc
    19021 gcctgtgtcc gcgcgagtgc ggcgcgaacc ggctcgcggg cgcacgcggc ttctgcggcg
    19081 cgacggcgga acccatggtc gccgcgcact acccgcattt cggcgaggag cggccgctcg
    19141 tggggcggca cggatcgggc gccgtcttcc tctcgaactg ctcgctgcgc tgcgtcttct
    19201 gcatcaacgc ggacatcagc caggggggcg cgggcgacac cgtcaccgtc gcgcgtctcg
    19261 cctcgatgat gctggagctc cagtcgaagg gctgccgcaa catcaacgtg gtcacgccca
    19321 cgcattactc cgcgcacatc ctgctggcgc tcgacgcggc ggcgcgacgc ggcctgcgca
    19381 tcccgctggt gtacaatacc tgcggctggg agcggccgga ggtgctcgcg cttctggacg
    19441 gcgtcgtgga catctatctg cccgacatga agtactggga ccccgagcgc gccgcgcgct
    19501 actcagccgg cgcgcgaagc tatccggagc tctcgcgcgc ggccatcgcc gagatgcacc
    19561 gccaggtggg cgtcgcgcgc ccggcggccg acggctcgct ctatcgcggc ctcatcatcc
    19621 gccatctggt gatgcccaac cgggtcggcg gcacgcgcga gatcctcgcg tggatcgccc
    19681 gcaccctgcc gaaggacacc tacgtgaacc tcatgtcgca gtaccggccc gtgcaccgcg
    19741 cgcgggaatt ccacgagatc gcgcgcggca tcacccgcgc cgaatacgcc gaggccgtgc
    19801 gctgggcccg cgaggccggc ctcacccggg tggacctgca gagagagccg ggcgcctgag
    19861 gagcgcatca tgccaaaaat tcgtgcgggg acggacacct tccgtcctcc gggcacctcc
    19921 tccttgtgaa gggaaaggaa gggcgggaat gtgtgcgata gagcgagcgc aatataatcg
    19981 cgttatacga caactctaac gatcgggacc catcggcgcg tatcgcacga taagcgcggc
    20041 gaccaggaac gagcagccga acgagaacgc gacatcggtc ggataatgcg cgcccgtcac
    20101 catgcggcag tacgaaacaa agagaccata ggcggaaacc gcgccaagaa gcgcgagcgc
    20161 cgccgcacgc ttctgcgacg cgaacacgta gaatacgagc ggcgcgagga tcagcgacat
    20221 cgcgacgtgg ccggacggga aggactgccc cgcgccgggg cccgccggct cgtaaaaatg
    20281 ggtgtagtcg agaaggccgg gcgacaggtc gcggaaacgc accctccccc agaggcgctt
    20341 gagcgcctgc gttatgagaa acggatggac gatcgcctga agcacgatga gcgtcgcgac
    20401 gtcccgatat cgcggtcggt ccgtgaaaag ccgcgcgtgg aacgcgagcg cacccaggga
    20461 gccgagaaag acgatccaca gcggccattc ccccgcgagc gcaacggccc gcgcgggtat
    20521 cgactgcgga ttgtacacgg cctgcgcaat cgtgaggtca tggggcgcta tcatgataag
    20581 cccgatcgcc cagacggcga ggccggccgt ccatgcggcc cagaaccgca tggccgacac
    20641 tgcgcgcatg tgtcgcatat gtgcgctctc cactacgtga cgttcgcccg gcggcggcat
    20701 gctcgtgcgt aaaaatcgac gacatgcgcg cgccgccgtg cgtcgtgact caggttcgac
    20761 ggaaaagcac tgcgcgagcg cgccggtccc gtcaagccgt taatgcgcgg tgcgtcggcg
    20821 aacgcgcgac agccgcatca ccaataaatg cttgcggata atcgcatcga tgcgcctgct
    20881 tgttccggga gcgaacacgc gcatgatcga catcggaaaa ctctactgcg ggggcgcctc
    20941 gaccggcgac ggcctccgct acggaacaga aagcggcgcg gattgccacg gcaaccggcc
    21001 gcaccgcgtg gtcgtgagcg cgcgcgagcg gcggcccatc gtggtgtgga gcgtcacgcg
    21061 cacctgcaat ctcaactgcg tccactgtta tacggactcc gcgaaccggc ggtacgcgga
    21121 cgagctgtcc accgacgagg gcctgcgcct catcgacgat ctcgcggcct tcggcatccc
    21181 gtcgctcctc ttctccggcg gcgagccgct catgcgcagg gacctcttca cgctcatcga
    21241 gcgcgccagc gcgcgcgaca tccgcccggt gatctccacc aacgggacgc tcatcgaccg
    21301 cgacgccgcg cgcgccatca aggactccgg catcgtgtac gtggggatca gcctggacgg
    21361 catggaggat gtgaacgacg agtttcgcgg ccagaagggg gccttttcga aagccatgaa
    21421 gggcttcgag aactgcgtgg ccgtgggcca gcgcgtgggc ctgcgcctca cgctcacgcg
    21481 gcacacctac gcggacctgc accgcatctt cgatttcatc gagcgcgagg gcatcaaccg
    21541 cgcctgcttc tatcatctgg tgtactcggg gcgcgggagc ggcatcttcg ccgacgacct
    21601 cacgcatggc cagtcgcgcg acgcgatgga catcatcctg gcgcgcacga aggacttctt
    21661 cgagcgaaag cgcgacatca acattctcac ggtggacaat cacgcggacg gggtgtatct
    21721 gtacctgaag cttcgcgagt ccgatccgca ccgcgcggag gaggtgcgcg agcttctcgc
    21781 ctggaacggc ggcggcgcgc actccacggg cgtggggatc gggaacatcg acttcgtcgg
    21841 gaacgtgcac cccgaccagt tctggcagga ttattccttc ggcaacattc gcgagcgcag
    21901 cttcggcgac atctggaccg acgagagcga gtcgctcatg aagggcctca agaacaaggc
    21961 cgactacatc aagggccgat gccgcctgtg cgcgcaccgt gccctgtgca ccggggccat
    22021 gcgggtgcgc gcgtatcgcg tataccacga cccgtgggcg ccggatccgc agtgctacct
    22081 taccgacgag gagatcggcc tcaccgacga gatgaagcgg gagcttgccg cccgcggcga
    22141 atcattcccc atgccgccgg agctcgcgag gtaggcgatg gccgtatccg aacagtgcgc
    22201 aatttgcggg gccgcgctca cggaacccca ttccaattac ccgcgcgccg tatgccccgc
    22261 ctgcgacgcg cgcgccctga atgcccaggg cgagcccgcc cgccacatca cccagacagt
    22321 gcctgccgag gaggcgcacc gatacgcctt caccgacgac ggcgacaatc cggttttcat
    22381 cgacggaatc cgctgcaccc ggcgctacaa attcggcggc tgggtgagca tgcgagacga
    22441 tcagtgatgt tccattcaac tatccgcgac ggagcgcgcc gatgaagcca tatcccgcat
    22501 ttctcaatct cgaagggcgc agcgtcgtca tcgtgggtgg gggcggcgtc gcgctgcgca
    22561 aggtgaagga cctgctcgac gccggggcgc gcattacgct tgtcgcgccg tcggtgctgc
    22621 cggagatcgc ggcgatcgcg gccgcggaac cggcgcgcgt cgccatccgc gaacgcgcct
    22681 acgagcccgg cgactgtgcg ggcgccgcgc tcgtcatctc ggcgaccgac gacggagaag
    22741 taaaccgcgc ggtcttccgc gaggccgagg agcggaatct cttcatcaat gccgtggacg
    22801 atcccgagaa ctgctccttt atcgtcccct cgacgatccg ccggggggac ctcctcatcg
    22861 ccgtctcgac gggcggcctt tcgccctcga tggccgcgcg gttgcgccgg gagctcgagc
    22921 gccatgtccc cgaggacatc gaggtgaagc tcgaggcgct cgccgccgcg cggaaggccc
    22981 tccgtgagaa cccgcgattg tcgcatctca cgtcggccga gcgaggcgag gtcctcaaat
    23041 acatcgccaa caacgacgag gtcctcaaca agctcatcac cgcgaccatt gccggcgagg
    23101 tggtggaatt gctcctgacg atcgtggggc ggtaggggag tgccgatcat taccatcaag
    23161 gaaactccgc ccctttccgc aatgcgtcaa gaaacatact gccggttatg agaactggat
    23221 cagcccgtca gacagtcctc ttacagccaa aaagcgtgtg acatcggtct ttaaatttat
    23281 cccaccaggt gtttttttcc gttatgttat gttgaaacct aaaacttttt ggcgttggtt
    23341 tagagcattt atgtttcaac ccaacaaccc aacatattcc cgaggccgct ctgagcctct
    23401 gattttgtgt ccattttttt ctagctgaaa agccccggcc ggccccatgg cccctcagac
    23461 tctacggaga ccctccccag tgatactaag taatagacca gacccatgcg gctcgctcct
    23521 ggagttgtta aaatctacca tcaaggaaac tccgcccctt tccgcaatgc gtcaagagac
    23581 atactgccgg ttatgagaac tggatcagcc gtcagactgt cctcttacag ccaaaaagcg
    23641 tgtgacatcg gtctttaaat tttgccgatt caccgggatg tgcgagaatc cccgtgtgag
    23701 ctccgaaagc ctgcagtgtc atgaaaacaa ccacgaatcc ccagatactt ctccaggtcc
    23761 cacggcccgg cttcccagct ggtgcgtggt aacacgattt gtgctctccc gacgtgtaaa
    23821 ggcacgggtt tcccttacat cagaaaccct ttagcatgat atttttgtat aaactcaata
    23881 ttcagtcctg tcggactgct tttatgccat ttttttttga gaaatattga cgacggtgga
    23941 attttttgat atattagtgt ttactaactt ttgaaagagg cacagatgaa cacacggccg
    24001 gaacgtaaag acgaaatcat tgcggcatcg cttgccctta tagaaagaaa ggggattcag
    24061 aatctcacca ctaaaaatat ttcacgggaa gtgggcataa cggaaccggg gatttaccgg
    24121 cattttaaaa acaagcagga gatccttacg gagattttaa ggcggtttaa aaacggaata
    24181 ggggagagga tgataatcgc aaaggaaagg gcgacgaatc ccctggatca gataggagca
    24241 ctttatgaag gctcgctgcg aatgttcaac gagtatcctt ccatcgccgc agcgattttt
    24301 tcagaggaga tattccagaa caatgaccgc ctttcttccg aagtgaggtc cattatggag
    24361 ctctctcagt cgggtttgcg ggacatgatt cgacgcggac agtccgaagg atgcattaga
    24421 aatgaccagc cggatgagca gttgtccctg atagtcatgg gagctttcag gctgctcgtg
    24481 acacggtggc gattgatgaa gaggtctttc gacattattg cggacggcag aaaattatgg
    24541 aaaacgatga gggcgttact tgcagtgaaa taacacggat agcgacggat ttgcgtccag
    24601 agcataagga tttattataa tgaacacact tgtccggttt ttcatcaaca gacccatgct
    24661 gatcaacatc atcattctgg cggtgatcgg actgggcgtg aaaagcgtca tggaggcccg
    24721 caaggaaggg ttccccgaaa tatcgatgaa taaaataatc attcaaacga tatatcccgg
    24781 ggcatcggcg agggacgtgg agctcaatgt aactgtcccg attgaggacg cgcttgaaga
    24841 acttgagggc atcaaagagg tcctatcggt ttcggaggaa ggagtttcaa gaattgaggt
    24901 aca
//
LOCUS       DAORHF010000031        24703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1281_length_24703_cov_6.307692, whole genome shotgun sequence.
ACCESSION   DAORHF010000031 DAORHF010000000
VERSION     DAORHF010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24703
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1281_length_24703_cov_6.307692"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PKJ16_04560"
     CDS             complement(<1..600)
                     /locus_tag="PKJ16_04560"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNV46293.1"
                     /translation="MIAAYLAGTTPPATAFAWTDIFAFVTPVKAGPDDTTLFFTLGGL
                     YIKKIGNTDFLTSNVKADFRFDDNITELQLYYDAFYTEVREKITEKKQSGTFRFDRRV
                     FSRFEGFVYSRSESNEITKILHRNNTGAGIKFDVIRNRYWRLDLSGAPVHQHEHIRDR
                     DPTDQWRWSLRFKSIIVPVRPVSLRYVFFYIPKIDESAVY"
     gene            complement(696..3857)
                     /locus_tag="PKJ16_04565"
     CDS             complement(696..3857)
                     /locus_tag="PKJ16_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46294.1"
                     /translation="MEKNILQTIKLEISKNFKLIPYERIAFHKILAILRTENGMNVLV
                     RDLAGDPLVRESAIAALRDFENPAVAAALAPLLDADTSMAEKITILDHIERRGSVGHA
                     GEMIAFIEAHRADQKLAPAVLKAFDVLRAVGGDDDGVYDFFLRTARDAEGDIALRRAA
                     VLGLSSFRRALVYEELLKENAPGVAQAVYQSIARYCENFARSAAEDSEDEQLFTYSSE
                     KEDGSILNIRVLLSKMTPQFDEYSDETKVAFLDAMIACAHREFLIYIMKALTSRNRAL
                     VIMALHLVRARVHRLADPDKLFRNLIALSVESAIENAVIVEIFQRFFTGMQENRKNLL
                     LRDKLFNYIVVTLETYFETYRKEFMVAEVAEKDFPENFRRIRHFILERFTPELRRALI
                     AYLKNEDRTGIHKIIVAIAGSLPHVSTEEGETVEFLVEVLFDNDPKAREVSAGRIESV
                     NFEKKFLRDRIVRLLRIIGILDIENAATALVKMFNYVKKYPDPEILNEVGETLSYLKY
                     SYMLGELEVALATENTVEQRRSIQLLAQFSDQRSLNIMTDFIKDKIAQSSPLTASILN
                     ILLTRDLRGNVAAGHIFRKIIEENTDAEIRRLAIQCLGKCGNESDIDFLNGIFYTLGP
                     GDPKEAIVQAIGAITELTANFNRRQVINYLQEYLKDPGIKVRIYSCAVLILMGNKGAI
                     KIIQDMMIIKNKQIQREILSIVGSLRSVEFAYFLISLMKEDYAISDDIVPILSLLPPE
                     ELLEIDHFIVNLFKKYEVPDSLITDRSHAAGTAEEKALFKRVESQAVTILRVSIRGFS
                     ELAGRLKLADLIMLHTVLNAYAIAPIQAGRGVVSRMCEGIVVAYFPDPLAASSAAMTI
                     LSNLREFNRNQSLHNAIAIEAQLMTDTAKIIQGEVLSASAYRIESMTTAGLANRILFD
                     EATGRAIENAFHAERMPGDIFSARGSIGAFYELVSPRNFLDAAESMIQKLQKIEQSRQ
                     ELQMQIDLELKKHKQEQRSPTAIAYAQMLDGFGKILKSELEEVNKYVQKRSLDRDLIG
                     TVGKMLQNIHKRYIVETSKIVIK"
     gene            4048..5295
                     /locus_tag="PKJ16_04570"
     CDS             4048..5295
                     /locus_tag="PKJ16_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46295.1"
                     /translation="MKAKKWTLYVVLFATVALLSCGTGQKKDGGKDAGSTRDELCGEG
                     TGYAQIYDNDVSMARDRALDEAMNFLVKAKLGQVVSGTSVAEDFALVASIVEARSSGM
                     VKDVKVLKEGPEQGAFVMTIKGCVYPQAVNDTIASTIRNYGRPKFMVLIRERFEGKAN
                     EPGFTVTELTMMEIMGKAGFEFVDAAMTQQLMRQDRSAISKAMAGRIDPSVQHLLLDR
                     KDIGAEVVIVGTAETKDQSHAMARVSKNMKSKSAIINVKAIDVYTGNILASTSANAPG
                     LHIESETASKTAIQRCLQQILGKEDEDDDGKFKSGPFMNQITKKFLRAATEREINMTV
                     TGLDNTAATKFRSELKGRIRGVKEANLKGQSGIQTKIQVIFAGKTDQLVQELQAKSRA
                     IGFDITVNEQFPNKVMLGAKALK"
     gene            5481..6683
                     /locus_tag="PKJ16_04575"
     CDS             5481..6683
                     /locus_tag="PKJ16_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HNV46296.1"
                     /translation="MPFTRPQLERYADVLIWGLTVARKKPFKPYDLILVRYDLEALPL
                     LETLYARLIAQRRNVVVRAMPGPSMEKSFFSLSDAAQRRFLAPFDMDLYARISGNIYL
                     HAPSSLTHLKSIDTQRINEFTISRKPLRDIANTREDAGAFTWTLCTYPTRALADQAQL
                     SLREYAAQVAKACFLGDRDPVGRWNRIYADVNGIKKRLNALPIESVTVESASCDLTIR
                     FGEKRRFIGVSGRNIPSFEIFTSPDWREARGVYFADQASFRNGNYVEGVRLEFQKGRV
                     VRVSAKKGGAFVKKTLSMDRGASQIGEFSLTDRRFSKIDRFMADTLFDENFGGRHGNC
                     HIALGSSYADAFAGDPATLTKSSKASLGFNDSALHWDLVNTEKKRVTAALRGGGKTVI
                     YENGMFAV"
     gene            complement(6688..7698)
                     /locus_tag="PKJ16_04580"
     CDS             complement(6688..7698)
                     /locus_tag="PKJ16_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46297.1"
                     /translation="MDLHEAQQTANWTRYVPGQRQGHYESFFLRANHPSRPLAFWIRY
                     TIFSPHEHPERAMGELWSVFFNGETGEHVAAKKEVPFSECSFARDRLEARVGDATLAS
                     GRLRGAITNAKTGGTIEWDLSYAGDHTPIFWFPLKFYTGGFPKAKGLVSLPLASFMGS
                     IVINGATVAIDSWVGSENHNWGIKHTDYYAWGQVAGFDNAPGSFLELATAKIKIGPIW
                     SPFFTPIVLRHDGREYALNELHTLLFRASFSYFHWNVATRSTEIDVRGVIRAEARDFV
                     CLPYYNPPGGVKMCLNSKIASCELDVTFRRTGERVRLVTAHRAAFEILTDKTDHGLLP
                     AV"
     gene            7889..8389
                     /locus_tag="PKJ16_04585"
     CDS             7889..8389
                     /locus_tag="PKJ16_04585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HNV46298.1"
                     /translation="MKTRIVGILFICIILIALPLAASPRRAQGFALLNRNNQLVTLSA
                     LMKKGNVIVSFWATYCVPCKREMPELSKLAAAYRDRKRVGVLFVSIDKEGKSVAQPFL
                     DALNIADECVFDIYQETAKRYIPSLKVPAVFLVNEKGMIVFEAVGETPETLRRLEDAI
                     KLLPDR"
     gene            complement(8606..8794)
                     /locus_tag="PKJ16_04590"
     CDS             complement(8606..8794)
                     /locus_tag="PKJ16_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46299.1"
                     /translation="MGSDTGLAVGVDERRAPELYGTYGVVLRVLGDDDRYVELLLEER
                     ELEDREELDERELDELER"
     gene            8820..11321
                     /locus_tag="PKJ16_04595"
     CDS             8820..11321
                     /locus_tag="PKJ16_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNV46300.1"
                     /translation="MKSYTAVLAIARDGSVTVSETLSVMLASPANGITRSLVPDEQYT
                     GLIAADTVSTTTGSVTYSNARYFQVGNPNTKLQKEQTFTLSYRLDGIVLPYAAGPVLA
                     WRMNLGRPLPQGAITIRFPDAASAARCEVVIVPAKPHRFYTATKLKTEVRGAEVSAAI
                     ERPLKDWETVEAYAEFPAGSVDPVALAAAFDKRFGKNTIYSIARLAYRATVRRNLSVD
                     VDAAYDLSIPNKYARPCQARRETYSVGIPYAEQSKPDKDSWRRDLYLYNLSGSGYTDR
                     TLSERDICYTLAPGTSAQLKTGYTTYGNFYDTGTLFVDLELPGLDAKRTGVITVSIDV
                     PDGVSTDLVRPQFLLKRTANNELLLRPLAVPFTWEKNRLTATIGGLLEDQTIMVRLFL
                     PADAFTRPFIAARAIMYVRTMWNFNTGLLIAVIVVVLVIGAIIVISIMAVRSGRPLPS
                     LPKIAKREKKKAPAASIAIPPAIEAQIREHDPAFTAAHFLSRVTAISTRLQEAWGRGD
                     MHPVRNYVSQGVYNRFRLQLDLMIHEEGVIDCTSDFAITDARVTGISSARNYQTLHVR
                     ITAQIRDLTIDASLSDDDKRKLLAEEPLETFTEIYSFTRRTGAKTDTTKDWLKGQCPN
                     CGFVPENFSEVNKCPNCATIYNSGEFEWVLSEITQLEEWRPTSCQDIPGLAALEEKNL
                     SINREVIEDRASYLFWRWVYARAKGMPSAIARDATKEFLQAFSAQRSAISEIAVGAVD
                     LERVSQDGDTVRAVVAVKWSAARARDAEPEYREHAFTLMLPVATTNEYGLAEHSCPNC
                     GAPLPELDALTCAYCGADLPSAVNDWMLESVEQRA"
     gene            complement(11342..12064)
                     /locus_tag="PKJ16_04600"
     CDS             complement(11342..12064)
                     /locus_tag="PKJ16_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46301.1"
                     /translation="MDAGTRLTKRIGEAAGAAREIFELIRRHKTEVMARFLGTSPDVT
                     DPAHAPARDGKWPVFPLREISLIVHGDLRAEDDRRGPAPGSVLIAVPSCVSSLRFSDD
                     ACATVDPSHIAPGTPRAEAGDIVVACAGDHPGASAILPLDHAPIALGSGCAAVRADGG
                     RCDQFYLLNVLHFFHTTGRMPSTRDDLASLRVPLPPHDEQRRITHYLLELSGGMVAQE
                     RHEDSLARLAALVRDAEAVLRG"
     gene            complement(12072..12452)
                     /locus_tag="PKJ16_04605"
     CDS             complement(12072..12452)
                     /locus_tag="PKJ16_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46302.1"
                     /translation="MRIAFEIIRITQRLDEVIDFNALIFRIDAPKRVDIDSSRYLWIF
                     LKTVIDGGVRKIIIDMKNLEMIDSSGMGVLINAAKLVRGKKGDIILSNVSADVRKIFK
                     IINLQEFIKMFNTDGEAQNHFRFL"
     gene            complement(12465..12845)
                     /locus_tag="PKJ16_04610"
     CDS             complement(12465..12845)
                     /locus_tag="PKJ16_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46303.1"
                     /translation="MKIAFEIIKTTKRTDESIDYKTIVFKLVLEKIIDLDSSRDLGAL
                     FEALVDGGAVKILLDMTGLEYIDSMGIGTVISSAKLVRKKRGDVVMIGVTPDLDKIFR
                     VVNLHRVIKMFDTEDEALNFFRYV"
     gene            complement(12881..13270)
                     /locus_tag="PKJ16_04615"
     CDS             complement(12881..13270)
                     /locus_tag="PKJ16_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46304.1"
                     /translation="MSACNTTFSVITLGSARNDAIDYRALVFRVGPGAVLDADTAPSV
                     WVLVKTLIEGGARKFVVNMNGLEFIDSYGIGIFVNAAKTVRGRGGDLALAEVPPSIAR
                     ILEPINVARFIQIFAAEQDALDYLGGL"
     gene            complement(13293..14867)
                     /locus_tag="PKJ16_04620"
     CDS             complement(13293..14867)
                     /locus_tag="PKJ16_04620"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HNV46305.1"
                     /translation="MAKEKRGDCILAIDAGTQSIRAIAVDLKGNLYDSVKTPLDPYFS
                     TNPGWAEQDPEYYWKILCQTTKKFFAKTKVPRDAFTGVTLTTQRNTVINLDKNGKPLR
                     PAIVWLDQRKARNESWPSLPLRTALRAINMLETVEHSYRECEANWIRQNQAEIWGKTD
                     KFVFLSGYFINRLIGEFVDSTASQVGYIPFDYKTHAWAAPSDMKWKMFPIEPEKLVRI
                     VEPAELMGAITKKASQETGIPAGLPLVSAGADKACEVLGAGCLSPEIACLSYGTTATV
                     ETTNEKYVEVIPFLPPYPSAVPGSYNTEIMIYRGFWMVSWFKKEFGLREQQIAQKRKI
                     PPEALFDELIRDIPPGSMGLTLQPYWTPGVKVPGPEAKGAIIGFGDVHTRAHIYRAIL
                     EGLAYALKEGALRTEKRNKVKIEKIRVAGGGSQSRVAMQLTADIFNMPAEKPHTPETS
                     ALGAAIDAAVGLKYFTDFSGAIRSMTRVGEVYEPAGESARVYQELFEKVYLRMYAKLQ
                     PLYDDIRSITGYPARH"
     gene            15025..15792
                     /locus_tag="PKJ16_04625"
     CDS             15025..15792
                     /locus_tag="PKJ16_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HNV46306.1"
                     /translation="MPVLTEDARAVARDHYLLSIAFDAIDAAPGQFVNIKAGSNADPL
                     LRRPFSIYDRAGSVLEIVVRVVGRGTAWLSGCRAGADIDVLAPLGRGFTLAEDKRALI
                     VGGGVGNAPLQYLARELRARACRVTYLYGAASGEFVYCEDAFRAASDDFLLTTDDGSA
                     GRRGRITDALAEVLAERAFDIAYVCGPTPMMRAAAALLAPRDIAFELSVENYFGCGIG
                     VCSGCSIGTTDGLRRACVDGPVFDGRAIHWESLEAQS"
     gene            15789..16592
                     /locus_tag="PKJ16_04630"
     CDS             15789..16592
                     /locus_tag="PKJ16_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46307.1"
                     /translation="MKPFVILIAFALSGCITASYHTATVEFGHAYREFDRDHRAATRM
                     YTGTMAGRCDINGEGFHRFQFAGILKGAGRYLDVLVPERERMTPAYETGPDGILRESK
                     RLIKRSGLIRIRESATPAPPGPPAYLAFTTMSTMTMKTFTALMNQEVGKPSDPISFLN
                     EHFSFAVVRAEYPLAIIHLDFSLNYSYSARAVIWGAPADGSSLVICTGIAVGPYTDPH
                     FDIAWKERSRIVYAARMAGYVGTVIADVVTSPVQLVMTILTALTGPGVR"
     gene            16742..18439
                     /locus_tag="PKJ16_04635"
     CDS             16742..18439
                     /locus_tag="PKJ16_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HNV46308.1"
                     /translation="MNGFIIAITVLGGLGLFLFGMKIMSEALQKATGDKLRNILTRVT
                     SNRFFGLLTGLGITALIQSSSATTVMLVSFVNAGLINLSQSVGIILGANIGTTVTGWI
                     VALLGFKVQITMFALPAIAIGFFLRFFKNEKFEDWGEVILGFGILFLGLDIMNSAVKD
                     LRGSSAVMHLMSMVRADTIPTAILVVVIGTLVTMVVQSSSATMAMTMALAVNGIVDFY
                     TCCALILGENIGTTFTAFLASLGASTTARRTARVHMLFNIFGVIWVLSCFHWLFIPFI
                     NWVVPGDPFSTVASERSKVIADHMAAFHTLFNVINAMLFLPFTTVLANIATRLVPDKA
                     GTAEYDEFNLKYIASPLLSTPAININQARMETQRMVGISIEMFDMVMEVFGKPGEKLG
                     ELVEKIQVRERTIDKLEMEISEYLVKISQESISQDQSYEISMLLHAVNDCERIGDHCE
                     NLLKLVRRKYDGKLEFSDRAVQEITSISAKVRDLLVLIHDGLMKASVSIMTTASVLEN
                     RINELRKELRSTHISRLNEGACDVSSGLVFIDMLTSFEKIGDHAFNIAEGIALKKIT"
     gene            18589..19518
                     /locus_tag="PKJ16_04640"
     CDS             18589..19518
                     /locus_tag="PKJ16_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV46309.1"
                     /translation="MNTFIPRLLCACALLVPLAAFAGGSAEKKCNTKYPIILSHGMGV
                     RDSILGVNYWYGIPDALRDEGARVYISNQNAFGSHAQRGEQLRAYIMGVLALYNYNTK
                     VNIIGHSQGTLDSRYVIATPITYTVGGKTYTRKMSDFIASWTGIAGPNRGSAVADVIL
                     GALPNGTHTTVGAIINAAGWLMGDVKPDSLGAARNLTRDYMCNVFNKAYPDQPGVYYQ
                     SWAAKIKTLTADCVLIEPTHLILKFYEGDNDGLVSVSSAKWGNFRGVVSGAWWCGGVS
                     HFNIVDQFFGVTPGFDAPAFYVDIVNDLKKRGY"
     gene            complement(19695..20069)
                     /locus_tag="PKJ16_04645"
     CDS             complement(19695..20069)
                     /locus_tag="PKJ16_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV46310.1"
                     /translation="MNSTNTHVAEQNRYEREIVPAVDIYENETGFVVTVDMPAVSKEN
                     VAVTLHENTLDIRGTVTADEKERESLRYGEYALYNYHRTFTVGDDIDPNGVSAKIENG
                     VLTLTLAKREEVKPKKIEIAVH"
     gene            complement(20081..20383)
                     /locus_tag="PKJ16_04650"
     CDS             complement(20081..20383)
                     /locus_tag="PKJ16_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV46311.1"
                     /translation="MNVYHAGDAVEVRALVPGVTADTITLELVDRDLVIKGARTAANE
                     QHAYIRREREFGEFTRSVRLPIAVDRDSITATLKDGVLRVTLAASEAAKPKRIAIQ"
     gene            complement(20618..21808)
                     /locus_tag="PKJ16_04655"
     CDS             complement(20618..21808)
                     /locus_tag="PKJ16_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV46312.1"
                     /translation="MDSIGKKILSAPAIAIAVIVAIACVVVLSASRLTGAGGGFGADG
                     KSPISSSSSRAWDMQKTFREIYDLNNDRVVFISTEQTVQMPQNPFFNDPFFRDFFGMP
                     RGQQQPRTQKRTGLGTGFVLTDDGYICTNAHVVSRMDRTGNIIGPLERVKVKVGDTTY
                     DAIVVGADGRTDIALLKINPKTKLNPVYLGDSDTVRVGDWAVAIGNPFGLDKTFTVGV
                     ISAVARKDVGMMGGSQTHIQTDASINPGNSGGPLININGEVIGINRMIYSNSGGSLGI
                     GFAIPINQAKAVLEDLKKFKKVKRGYIGVHIAPLTDEHVKELGLKNTEGARVDAVLNG
                     GPSAKAGMRIGDVILSVDGTKVNSPGALIEIVEKMPIGKTLKIEVWRERGTVTLYITI
                     QERS"
     gene            complement(22025..22450)
                     /locus_tag="PKJ16_04660"
     CDS             complement(22025..22450)
                     /locus_tag="PKJ16_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46313.1"
                     /translation="MKLAPFSHRPEMLIDTEQNSRMNRVNRWDATGARTVTINPDRER
                     RRGRDHARTDDTPDEAGAHLESLRNMAAQVNEDLASRNSPHRFYVFEEGGEVFINLVL
                     LDAAGSVVRIIRKDITHSEFSDLVRQLERGEGLFLELVG"
     gene            complement(22661..24472)
                     /locus_tag="PKJ16_04665"
     CDS             complement(22661..24472)
                     /locus_tag="PKJ16_04665"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HNV46314.1"
                     /translation="MNDSSLTKHNGLIQWVDAMAAMCQPDRVYWCDGSQREYDAMIKI
                     MVDCGLATPLNEKTRTKSYLFRSDPSDVARVEDRTYIASKSKDEAGPTNNWIDPDELK
                     KTMKGLYTGCMKGRTMYVIPFSMGPVGSPIAQIGIELTDSPYVVVNMHIMTRVGSAVL
                     DALGENGTFIPCLHSVGKPLGPGESDGHLWPCAPIEKKYISHFPEEKTIWSFGSGYGG
                     NALLGKKCFALRIASVMAREQGWLAEHMLILGITNPQGVKKYIAAAFPSACGKTNLAM
                     LIPSIPGWKVECVGDDIAWMKFGEDGRLYAINPEAGFFGVAPGTSMDSNPNAMKSIDR
                     NTIFTNVALTENNDVWWEGIGYDPPAGRIIDWTGAEWTKDSGKLAAHANARFTAPARQ
                     CPAIDPNWEDPKGVPISAFLFGGRRPTTIPLVHQAFNWNHGVFLGSIVGSEVTAAAIS
                     ANIGQVRRDPMAMLPFCGYHMGDYLKHWITIGSRTSAEKLPKIFYVNWFRKTADGKWL
                     WPGYGENSRVLKWVFERVNGEGKAQDTAIGHMPTVDAIDRSGLDVSDADMRELLAVDH
                     DEWKREIASIREHYARFGDRLPRELADELAALERRLG"
BASE COUNT         4710 a         7614 c         7428 g         4951 t
ORIGIN      
        1 atacaccgcg ctctcgtcga tcttcggaat gtagaagaac acgtagcgga gcgacaccgg
       61 acgcaccggg acgatgatgc ttttaaagcg aagcgaccat cgccactggt ccgtcggatc
      121 ccggtcgcgg atgtgctcgt gctgatgcac cggcgcgccg ctcaaatcga gacgccagta
      181 gcggttgcgg ataacgtcga acttgatccc cgcgccggta ttgttgcggt gcagtatctt
      241 cgtgatctcg tttgattccg agcgcgaata cacgaaaccc tcgaagcgcg aaaacacgcg
      301 ccggtcgaac cggaacgttc ccgactgctt cttttcggtg atcttttccc gcacctcggt
      361 atagaacgcg tcgtagtaca gctggagctc ggtgatgttg tcgtcgaacc ggaagtcggc
      421 cttcacgttc gaggtgagaa agtcggtgtt gccgattttt ttgatataaa gtcccccgag
      481 cgtgaagaag agcgtcgtgt cgtcgggccc cgccttgacc ggcgtaacga aggcgaagat
      541 atcggtccag gcaaacgccg tcgcgggcgg cgtcgtgccc gcaagatagg cggcgatcac
      601 gtggtcggtc gtgtcttgcg cggcggcggg acgggcgaac gcgagcgcgg cggcgcatgc
      661 agcgacgatc aggatgcggc gaaacgggaa cgcggttatt ttatgacgat cttcgaggtt
      721 tcgacaatat agcgcttgtg gatgttctgg agcatctttc cgacggtgcc gataagatcg
      781 cggtcgagag aacgcttctg gacgtatttg ttcacctcct cgagttccga tttgagtatc
      841 ttcccgaagc cgtcgagcat ctgcgcgtag gcgatcgcgg tgggcgagcg ctgctcctgc
      901 ttgtgcttct tcagctccag gtcgatctgc atctgcagtt cctggcggga ctgctcgatc
      961 ttctgcaact tctgaatcat cgactcggcc gcgtcaagaa agtttctcgg cgacacgagc
     1021 tcataaaacg cgccgatcga cccgcgggcc gagaagatgt cgcccggcat gcgctcggcg
     1081 tggaaggcgt tctcgatcgc gcgtccggtc gcctcgtcga agagaatgcg gttggccagt
     1141 cccgccgtgg tcatcgactc gatgcggtac gccgatgcgc tgagcacctc gccctggatg
     1201 atcttcgcgg tgtcggtcat cagctgcgcc tcgatggcga tggcgttgtg aagcgactgg
     1261 ttgcggttga actcgcgcag gttcgagagg attgtcatgg ccgcactcga cgccgcaagc
     1321 gggtcgggga aataagcgac gacgatcccc tcgcacatgc ggctcaccac gccccgcccc
     1381 gcctggatgg gggcgatcgc gtacgcgttg agcacggtgt gcagcatgat gaggtcggcg
     1441 agcttgaggc ggccggccag ttcggagaac ccgcggatcg agacgcgcag gatcgtcacc
     1501 gcctgggatt cgacccgctt gaagagcgcc ttctcctccg ccgtgccggc cgcgtgcgag
     1561 cggtcggtga tgagcgagtc ggggacctcg tacttcttga acaggttcac gatgaagtgg
     1621 tcgatctcga gcagctcctc aggcggcagg agactcagga tgggcacgat gtcgtcgctg
     1681 atcgcgtaat cctccttcat gagcgagatg aggaagtacg cgaactcgac cgagcggagg
     1741 cttcccacga tggacaggat ctcccgctgg atctgcttgt tcttgatgat catcatgtcc
     1801 tggatgatct tgatggcgcc cttgtttccc atcaggatga gcacggcgca ggagtagatc
     1861 cgcaccttga tccccgggtc cttgaggtat tcctgcaggt agttgatcac ctgccggcgg
     1921 ttgaagttcg cggtgagctc cgtgatggcg ccgatcgcct ggacgatggc ctccttcggg
     1981 tcgcccggcc ccagggtgta gaagatcccg ttgagaaagt cgatatcgct ctcgttgccg
     2041 cacttgccca ggcactggat ggccagccgg cggatctccg catcggtgtt ttcttcgatg
     2101 atttttctga agatgtgtcc cgcggccaca ttgccccgca ggtcgcgcgt gagcaaaatg
     2161 ttcaggatcg acgccgtcag cggcgagctc tgcgcgatct tgtccttgat gaagtcggtc
     2221 atgatgttga gcgagcgctg gtcggagaac tgcgccagca gctggatgct gcgccgctgc
     2281 tcgaccgtgt tctcggtggc cagcgcgacc tcgagctcgc ctagcatata ggagtacttc
     2341 aggtacgaga gcgtctcgcc gacctcgttc aggatctcgg gatcgggata cttcttcacg
     2401 tagttgaaca tcttcaccag cgcggtcgcg gcgttctcga tgtctaggat cccgatgatg
     2461 cgcagcagcc ggacgatgcg gtcgcgcaaa aacttctttt cgaaattgac cgactcgatg
     2521 cgtccggccg acacctcgcg cgccttcggg tcgttgtcga agagcacctc gacgaggaac
     2581 tcgacggtct cgccctcctc ggtcgagaca tgcggcagcg accccgcgat ggccacgatg
     2641 attttatgga tgccggtgcg gtcctcgttc ttgaggtacg cgatgagggc gcggcggagc
     2701 tcgggggtga atcgctcgag gatgaagtgg cggatgcgcc ggaagttttc gggaaagtcc
     2761 ttctccgcca cctcggccac catgaactcc ttgcggtagg tctcgaagta ggtctccagg
     2821 gtcaccacga tgtaattgaa gagcttgtcg cgcaggagca ggttcttccg gttctcctgc
     2881 atgccggtga agaagcgctg gaagatctcc acgatgacgg cgttctcgat cgcgctttcg
     2941 accgagagcg cgatcaggtt gcggaacagc ttgtcgggat cggcgaggcg gtgcacccgc
     3001 gcgcgcacca ggtggagcgc catgatcacg agcgcgcggt tgcgcgaggt gagcgccttc
     3061 atgatgtaga tgaggaattc gcggtgcgcg caggcgatca tcgcgtccag gaaggcgacc
     3121 ttggtctcgt cggaatactc gtcgaactgc ggcgtcatct tgcttaagag cacccgtatg
     3181 ttcaggatcg acccgtcctc cttctcggac gagtaggtga acagctgctc gtcctcggaa
     3241 tcctcggcgg ccgaccgcgc gaagttttcg caatagcgcg cgatggactg gtataccgcc
     3301 tgggcgacgc cgggcgcgtt ctccttgagg agctcctcgt agacgagcgc tcttcggaat
     3361 gaggaaagcc ctagaacggc ggcgcggcgc agggcgatgt ccccctcggc gtcgcgggcc
     3421 gtgcgcagaa agaagtcgta caccccgtca tcatcgcccc cgacggcgcg caggacatca
     3481 aaggccttga gcacggccgg cgccagcttt tggtccgccc ggtgcgcctc gatgaatgcg
     3541 atcatctcgc cggcgtgccc gacggagccc cggcgctcga tgtggtcgag gatggtgatc
     3601 ttttccgcca tgctggtgtc ggcgtccaga agcggcgcaa gcgcggcggc gacggcggga
     3661 ttctcgaagt cgcggagcgc ggcgatggcg ctttcgcgca ccagcggatc gcccgcgaga
     3721 tcgcgcacga gcacgttcat cccgttttcg gtcctcagga tggccaggat cttgtggaag
     3781 gcgatccgct cgtaggggat cagcttgaag ttcttgctga tctcgagctt gatggtttgc
     3841 aggatgttct tttccatggg cgaacgtgta ccgatcaggc gcgtatggta tatacatatc
     3901 cgccgatttg gaccacgagc gcaatcaaaa aatgaattga cgttttttga actctttgta
     3961 ttctacttaa gctcccaaga gcggatcagc acataccggc cgggcccgct gggcgccggc
     4021 tgattatcat cgacaaggag ttttcccatg aaagcgaaga aatggacact gtacgtcgtt
     4081 ctttttgcga cggtagccct gctctcgtgc ggcaccgggc agaaaaagga cggcgggaag
     4141 gacgccggga gcacgcgtga cgagctctgc ggcgaaggga ccggctatgc gcagatttac
     4201 gataacgacg tctccatggc gcgcgaccgc gccctcgatg aggcgatgaa ctttctcgtc
     4261 aaggcgaagc tcggccaggt cgtttccggg acctcggtcg ccgaggattt cgcgctggtc
     4321 gcctccatcg tcgaggcccg ctccagcggc atggtcaagg acgtgaaggt cctcaaggag
     4381 ggccccgagc agggcgcctt cgtgatgacc atcaagggct gcgtgtatcc ccaggcggtc
     4441 aacgacacca tcgcctccac catacgcaat tacggccgtc ccaagttcat ggtgctgatc
     4501 cgcgagcgct tcgaggggaa ggccaacgag ccgggcttca ccgtgaccga gctgaccatg
     4561 atggagatca tgggcaaggc gggctttgag ttcgtcgacg ccgcgatgac ccagcagctc
     4621 atgcgccagg accgctcggc catcagcaag gcgatggcgg ggcgcatcga tcccagcgtc
     4681 cagcacctcc tcctcgaccg taaggacatc ggcgccgagg tcgtgatcgt cgggaccgcc
     4741 gagacgaagg accagtcaca tgcgatggcc cgcgtttcca agaacatgaa atcgaagtcg
     4801 gccatcatca acgtgaaggc gatcgacgtg tacaccggga acatcctggc cagcaccagc
     4861 gcgaacgccc ccggcctcca catcgagtcc gagaccgcgt ccaagaccgc catccagcgc
     4921 tgcctgcagc agatcctcgg aaaggaagac gaggacgatg acggcaagtt caagtccggc
     4981 ccgttcatga accagatcac caagaagttc ctccgcgccg ccaccgagcg cgagatcaac
     5041 atgaccgtga ccggcctgga caataccgcg gccaccaagt tccgcagcga gctgaaaggg
     5101 cgcatccgtg gcgtgaagga agcgaacctc aagggccaga gcggcatcca gacgaagatc
     5161 caggtgatct tcgccgggaa gaccgaccag ctcgtgcagg agctccaggc gaaatcgagg
     5221 gccatcggct tcgatatcac cgtgaacgag cagttcccca acaaggtgat gcttggcgcg
     5281 aaagcgctca aatagcgcgg gcgcaatcaa cacgaagacg gatgtacgat gcggcggccc
     5341 caccgggccg ccgtttcatt tgggcggtcc cgccggcggg gagcgcagcg acgggacgta
     5401 aaaagaattg aaaaaagacg gcgcgccgtc gagggtgatg acgccgtgtg gcatcacgat
     5461 accgccgtca tggaggaacc atgcccttta cccgaccgca actggaacga tacgccgacg
     5521 tgctcatctg gggcctcacc gtcgcgcgga agaagccatt caagccgtac gacctcatcc
     5581 tggtccggta cgatctcgag gcgctgcccc ttctcgagac gttgtacgcc cgactgatcg
     5641 cacagcgccg caacgtggtc gttcgcgcca tgccgggccc gtccatggag aagagcttct
     5701 tctcgctctc cgatgcggcg cagcggcgct tcctggcccc cttcgacatg gacctctacg
     5761 cgcggatcag cggcaacatc tacctccacg cgccgtcgtc gctgacgcat ctcaagagca
     5821 tcgacacaca gcgaatcaac gagttcacca tctcccgcaa gccgttgcgc gacatcgcga
     5881 acacgcggga ggacgccgga gcgttcacct ggacgctgtg cacctatccc acgcgggcgc
     5941 tcgcggacca ggcgcagctg tccctccggg aatacgccgc gcaggtcgcg aaggcctgct
     6001 tcctcggcga ccgggacccc gtcgggcgat ggaaccgcat ctacgccgac gtgaacggca
     6061 tcaaaaagcg cctgaacgcg ctcccgatcg agagtgtcac cgtggagagc gcctcgtgcg
     6121 atttgaccat ccggttcggc gagaagcggc ggttcatcgg cgtgagcggg cgcaacatcc
     6181 cttcgttcga gatattcacc tcgcccgact ggcgcgaggc gcgcggcgtc tacttcgccg
     6241 accaggcctc cttccgcaac ggcaattacg tggagggcgt gcgcctcgag ttccagaagg
     6301 ggcgcgtggt gcgggtgtcg gcgaaaaagg gcggggcgtt cgtgaaaaaa acgctgtcga
     6361 tggaccgcgg cgcgtcgcag atcggcgagt tttccctcac ggaccgccgc ttctcgaaga
     6421 tcgaccgctt tatggccgac acgcttttcg acgagaactt cggcggccgc cacgggaact
     6481 gccacatcgc gctgggcagc tcctacgcgg acgcctttgc cggcgatccg gcgacgctca
     6541 cgaaatcgtc gaaggcatcg ctgggcttta acgattccgc gctccactgg gacctggtga
     6601 ataccgagaa gaagcgcgtc acggccgcgc ttcggggcgg cgggaagacc gtcatttacg
     6661 agaacggaat gttcgcggtc tagcggacta caccgccggc agcaggccgt gatcggtctt
     6721 gtcggtgagg atctcgaagg cggcccgatg cgccgtcaca agccgcaccc gttcgcccgt
     6781 tctcctgaac gttacgtcga gctcgcacga agcgatcttg ctgttgaggc acatcttcac
     6841 gcccccgggc ggattgtagt acggcaggca cacgaagtcc cgcgcctccg cgcggatcac
     6901 gccgcgcaca tcgatttccg tcgagcgcgt cgcaacgttc caatggaaat acgagaagct
     6961 cgcgcgaaac agcagcgtat gcagttcgtt gagcgcgtac tcgcgtccgt cgtgtcgaag
     7021 cacgatcggc gtgaaaaacg gcgaccatat cggcccgatc ttgatcttcg ccgtggcgag
     7081 ctcgaggaag ctcccgggcg cattgtcgaa cccggcaacc tgcccccagg catagtagtc
     7141 cgtgtgcttg attccccagt tgtggttctc gctccccacc caagagtcga tggcgaccgt
     7201 cgcgccattg ataacgatgc tgcccataaa tgacgccagc gggaggctca ccagcccctt
     7261 cgccttggga aaaccgccgg tgtagaactt gagcggaaac caaaagatgg gcgtgtgatc
     7321 gccggcatag gacaggtccc attcgatggt cccgcccgtc tttgcgttag tgatggcgcc
     7381 ccgaagacgt ccggaggcaa gagtagcgtc gcccacgcgc gcttccagcc ggtcccgcgc
     7441 aaatgagcat tcggaaaacg gcacctcctt ctttgccgcg acatgctcgc cggtctcacc
     7501 attgaagaag accgaccaga gctcgcccat cgcccgctca ggatgttcgt gcgggctgaa
     7561 gatcgtgtac cggatccaga aggcgagcgg tcgagaggga tggttcgccc tgagaaaaaa
     7621 actttcgtaa tgtccctgcc gctggccggg aacgtagcgc gtccaattcg cggtctgttg
     7681 cgcctcgtga aggtccatat gcgctcctct ttcttacgat gaatagacac gtccgttcat
     7741 gccgcgcccc tggaacacac cgcgtatgcg gcatcatcaa cgcttataaa aagcatacgt
     7801 cgatagcgaa taaactcaac catattttct ttcttgcaaa aaattcatcg ggacagggaa
     7861 atatcggcga acagcggagg aacgtgacat gaaaacgcgc atcgtcggta ttctctttat
     7921 atgtatcatt ctgatcgcgc tgccgctcgc cgcctctccg cgcagggcgc agggcttcgc
     7981 gctcctcaac cgcaacaacc agctcgtcac gctcagcgcg ctgatgaaga aggggaatgt
     8041 catcgtctcg ttctgggcca cctattgcgt tccctgcaag cgggagatgc ccgagctctc
     8101 caaactcgcc gccgcctatc gcgaccggaa gagggtgggc gtgctcttcg tgagcatcga
     8161 caaggagggc aaaagcgtgg cgcagccgtt cctcgacgcg ttgaacatcg ccgacgagtg
     8221 cgtgttcgac atctatcagg aaaccgcgaa gcgatacatt ccgtccctca aggtgcccgc
     8281 ggtatttctg gtcaacgaaa aggggatgat tgtgttcgag gccgtgggcg aaacgcccga
     8341 gacgctccgg aggctcgaag acgccatcaa actcctcccg gaccgctgat cggcgcgtcc
     8401 ggcccacggg tccccatttt ttcctaattg acatatcgcc gatataatcg gtatgatttg
     8461 ccatcgccaa cgaaaaggag cggatcatgt cgattagcag cttgtcacga cgatccatcg
     8521 ttgccgccat ttgcgccctg tgcctcgtgg cggattttac catcttctcc gatgacgccg
     8581 acgcgcgcgc cggacgcggg ggaagctatc gctcgagctc gtcaagctcg cgttcatcga
     8641 gctcctcacg gtcttccagc tcgcgctcct cgagcagcag ctccacgtat cgatcgtcat
     8701 caccgagcac gcgcagcaca acgccgtacg tgccgtacag ctccggcgcc cgacgctcat
     8761 cgacccccac cgcgagaccc gtgtcgctgc ccacaacgac ctattcgctc gattattcaa
     8821 tgaagtccta tacggccgtt ctcgctatcg cgcgtgacgg atccgtgacc gtatcggaga
     8881 cgctcagtgt gatgcttgcc tcaccggcga acggaataac ccggtcgctg gtgccggacg
     8941 agcagtatac cggcctcatc gccgccgaca ctgtgagcac caccaccgga agcgtgacct
     9001 attcaaatgc gcgatacttc caggtcggca acccgaacac aaaactccag aaggagcaga
     9061 ccttcaccct gtcgtatcgg ctcgatggga ttgtgctccc ctacgccgcg gggcccgtgc
     9121 tcgcctggcg gatgaacctt ggccgcccgc tgccgcaggg cgcgataacc attcgcttcc
     9181 cggacgccgc ctcggccgcg cgctgcgagg tcgtcatcgt tcccgcgaaa ccgcaccgtt
     9241 tctatacggc gacgaagctt aaaaccgagg tccgcggcgc agaggtgagc gccgccatcg
     9301 agcgcccgct gaaggactgg gagaccgtcg aggcctacgc cgaattcccc gcgggcagtg
     9361 tcgatcctgt cgcgcttgcc gcagccttcg acaaacgatt cgggaaaaac acgatctatt
     9421 cgatcgcacg acttgcgtat cgggcaacgg tgcggcggaa cctctcggtg gacgtcgacg
     9481 ccgcatacga tctctcgatt cccaacaaat atgcccgtcc gtgccaggcc cgccgggaga
     9541 cgtattccgt cggtattcca tacgcggaac aatccaaacc ggacaaggac tcgtggcgtc
     9601 gcgacctcta cctgtacaat ctttccggtt ccggctacac cgaccgcacc ctgtccgaac
     9661 gggatatatg ctatacgctt gcgccgggta ccagcgcaca attgaagacg ggctatacga
     9721 catacggaaa tttctacgat accgggaccc tctttgtcga tctcgaactt ccgggcctgg
     9781 atgcgaaaag aaccggcgtc atcaccgtgt ccatcgatgt gcccgatgga gtgtcgaccg
     9841 atctcgtgcg cccgcagttt ctcctgaagc gaacggcgaa taacgaactc ttgctgcgcc
     9901 ctcttgccgt ccccttcacc tgggagaaaa accgcctaac cgcaacgatt ggcggcctgt
     9961 tggaggacca gacgatcatg gttcggctct tcctgcccgc agatgcgttc acgaggccgt
    10021 tcatcgcggc ccgtgcgatc atgtatgtcc gaacaatgtg gaacttcaac accgggctgc
    10081 tcatcgccgt catcgtcgtc gtactggtca tcggcgcgat catcgtcatc agcatcatgg
    10141 cggtgcgctc cggccggccg ttgccctcgc tgccgaagat cgcgaagcgc gaaaagaaga
    10201 aggcgcccgc cgcctctatc gcgataccgc ccgcgatcga ggcacagata cgggaacacg
    10261 accccgcctt cacggccgca catttcctgt cgcgcgtaac ggcgatctcc acgcgcctgc
    10321 aggaggcgtg ggggcgcggc gacatgcacc cggtgcgcaa ttacgtgtcg cagggcgtgt
    10381 acaaccgttt ccgcctccag ctcgatctga tgatacacga ggagggggtg atcgattgta
    10441 cgtcggattt cgccatcacc gacgcgcgcg tgaccgggat atcgagcgcg cggaactacc
    10501 agacgctcca cgtgcgcatc accgcgcaga tccgagatct caccatcgac gcctcgctca
    10561 gcgatgatga taagcggaag ctccttgcgg aagagccgct cgagaccttc acggaaatct
    10621 attcgttcac ccgccgcacg ggcgcgaaga ccgatacgac caaggactgg ctgaaggggc
    10681 aatgccccaa ctgcgggttc gtgccggaga atttcagcga ggtgaacaag tgccccaact
    10741 gcgccaccat ctacaactcc ggcgagttcg aatgggtcct ttcggagatc acgcagctcg
    10801 aggaatggcg tcccacctcc tgtcaggaca ttccggggct tgccgcactc gaggagaaaa
    10861 atctctccat caaccgagag gtgatcgagg accgcgcatc gtatctgttc tggcgctggg
    10921 tctacgcgcg ggcgaagggg atgccctcgg cgatcgcgcg cgacgcgacg aaggaatttc
    10981 tccaggcgtt ttccgcgcag cgaagcgcca tctccgagat cgccgtgggc gcggtcgatc
    11041 tcgaacgggt ttcgcaggac ggcgatacgg tccgcgcggt cgtcgccgtc aagtggagcg
    11101 cggcgcgtgc gcgcgacgcc gagccggaat accgcgagca cgcattcacg ctcatgctgc
    11161 cggtggcgac aacgaacgaa tacggattgg ccgagcacag ctgcccgaat tgcggcgcgc
    11221 cgctgcccga gctcgacgcg ctcacgtgcg cctattgcgg cgcggatctt ccgtccgccg
    11281 tgaacgactg gatgctcgag tcggtggaac agcgcgcctg acgcggccga ccgcgcccga
    11341 atcagccgcg caggaccgcc tcggcgtccc gaaccagcgc cgccaggcgc gcgagcgaat
    11401 cctcatgccg ctcctgcgcc accatcccgc cggagagctc cagcaaataa tgcgtgatgc
    11461 gccgctgctc gtcgtgcggc gggagcggga cgcgaagcga cgcaagatca tcgcgcgtgg
    11521 acggcatccg gccggtcgtg tggaaaaaat gcaaaacatt gagcaggtag aactggtcgc
    11581 agcgaccgcc atcggcgcgc acggccgcgc aaccgctgcc cagcgcgatc ggggcgtggt
    11641 cgagagggag tatcgcggag gcgccgggat gatcccccgc gcaggcgacc acgatgtcac
    11701 cggcctcggc gcgcggcgtt ccgggagcga tgtgcgaggg atcgaccgtc gcgcaggcgt
    11761 cgtcggaaaa gcgcagcgag gaaacgcaag aaggaacggc gatgagaacg gacccgggtg
    11821 cgggtccgcg gcgatcgtct tcggcgcgca gatcgccgtg cacgatcaga gatatttcgc
    11881 gcaacggaaa aacgggccat tttccgtccc gggcgggcgc atgtgcggga tcggtcacat
    11941 ccggcgacgt tcccaggaat cgcgccatca cctccgtttt atgccggcgg atgagttcga
    12001 agatctcccg cgccgcgccc gcggcctcac cgattcgctt cgtgagcctc gtccccgcgt
    12061 ccatgcgtcg ttcagaggaa ccggaagtgg ttttgcgcct cgccgtcggt gttgaacatc
    12121 ttgatgaatt cctgcaggtt gatgatcttg aagatcttgc gcacgtcggc ggagacgttc
    12181 gacaggatga tgtcgccctt ctttccgcga acgagcttgg ccgcgttgat cagcacgccc
    12241 atccccgagc tgtcgatcat ctccaggttt ttcatgtcga tgatgatctt gcgcaccccg
    12301 ccatcgatga ccgttttcag gaagatccac aggtagcgcg aggaatcgat atccacccgt
    12361 ttcggggcgt cgatccggaa tatcagcgcg ttgaagtcga tgacctcgtc gagtcgttgc
    12421 gtgatcctga tgatttcgaa tgcgatcctc atgggacccc cctgtcagac gtatcggaaa
    12481 aaattgagcg cctcgtcttc cgtgtcgaac atcttgatga cgcgatgcag gttcaccacg
    12541 cggaaaatct tgtcgagatc gggcgtgacg ccgatcatca ccacgtcgcc gcgctttttg
    12601 cgcaccagtt tcgccgagct gatcacggtg ccgatcccca tgctgtcgat gtattccagg
    12661 ccggtcatgt ccaggaggat cttcaccgcg ccgccgtcga ccagcgcctc gaagagcgcg
    12721 ccgaggtcgc gcgacgaatc caggtcgatg atcttctcga gcacgagctt gaagacgatc
    12781 gttttgtaat cgattgactc gtcggtgcgc ttcgttgtct tgatgatttc aaacgcgatc
    12841 ttcattccgt acccggatcg tgcgcggcat gcggcggcgg ttacagaccg cccaggtagt
    12901 cgagcgcgtc ctgctcggcc gcgaatatct gtataaaacg cgcgacgttg atcggctcga
    12961 ggatccgcgc gatcgacggc ggcacctcgg cgagcgcgag gtcgcccccg cgcccgcgca
    13021 ccgtcttcgc cgcgttcaca aaaatgccga tcccgtaact gtcgataaac tcgagcccgt
    13081 tcatgttcac cacgaacttg cgcgcgccgc cctcgatgag ggttttcacg agcacccaga
    13141 cgctgggggc agtgtcggcg tccagcaccg cccccgggcc gacgcgaaag acgagcgcac
    13201 ggtagtcgat ggcgtcgttg cgcgcgctgc ctagggtgat gacggaaaat gttgtgttac
    13261 aagcgctcat cggccgtgcg cgaatcctgt ttttagtgac gcgcgggata gccggtgatg
    13321 gacctgatgt cgtcgtacag cggttgaagt ttcgcgtaca tccgcaggta caccttttcg
    13381 aacagctctt gatacacgcg cgcgctctcg ccggcgggct cgtagacctc gcccacgcgc
    13441 gtcatcgacc ggatcgcccc ggagaaatcc gtgaaatact tgaggcccac cgcggcgtcg
    13501 atcgccgcgc cgagcgccga ggtctcgggc gtatgcggct tctcggccgg catgttgaag
    13561 atgtcggccg tgagctgcat cgcgacgcgg ctctgcgatc cgccgccggc cacgcggatc
    13621 ttttcgattt tcaccttgtt ccgcttctcg gtgcgcaggg ccccctcctt gagcgcatag
    13681 gcgagcccct cgaggatggc gcggtagatg tgcgcgcgcg tgtgcacgtc gccgaaaccg
    13741 atgatggcgc ccttcgcctc cgggccgggc accttcacgc cgggcgtcca gtacggctgc
    13801 agggtgagcc ccatcgagcc cggcgggatg tcgcgaatga gctcgtcgaa gagcgcctcc
    13861 ggcgggatct tccgcttctg ggcgatctgt tgctcgcgca ggccaaactc cttcttgaac
    13921 cagctcacca tccagaagcc gcggtagatc atgatctccg tgttgtacga tccggggacg
    13981 gcgctcggat agggcggcag gaaggggatc acctcgacgt atttctcgtt ggtcgtctcc
    14041 acggtcgcgg tggtcccgta actcaggcac gcgatctccg gcgataggca gccggccccc
    14101 aggacctcgc aggccttgtc ggcgcccgcg gagaccagcg gcagccccgc cgggatcccg
    14161 gtctcctgcg aggccttttt ggtgatggcg cccatgagct cggcgggctc gacgatccgg
    14221 acgagcttct ccggttcgat ggggaacatc ttccatttca tgtccgaggg cgcggcccag
    14281 gcgtgggtct tataatcgaa ggggatgtag cccacctggc ttgctgtcga atcgacgaac
    14341 tcgccgatga gccggttgat gaaatacccg gagaggaaca cgaacttgtc ggtttttccc
    14401 cagatctccg cctggttctg ccggatccag ttggcttcgc actcgcggta ggaatgctcc
    14461 accgtctcca gcatgttgat cgcgcgcagc gccgtccgca ggggaaggga cggccagctc
    14521 tcgttgcgcg ccttgcgctg gtccagccac acgatggccg gccgcagggg cttcccgttc
    14581 ttgtcaaggt tgatgaccgt gttccgctgc gtggtgagcg tcacgcccgt gaaggcgtcg
    14641 cgcgggacct tcgttttcgc gaaaaacttc ttcgtggtct ggcacaggat tttccagtaa
    14701 tactcggggt cctgctcggc ccatcccggg ttcgtggaaa aatacggatc gagcggggtt
    14761 ttcaccgaat cgtacaaatt ccccttcagg tccaccgcga tcgcccgtat ggattgcgta
    14821 ccggcgtcta tcgcgagaat gcagtcaccc ctcttttcct ttgccaccga acacccccta
    14881 ttcgtatata caaaatgcga atgcgtatca tatgggcgcg cgcatttttt tcaataacaa
    14941 tttatgttga taaaaaacga cccgtcgcat agcgtgcgtt catccggcgg gcagcgcgcc
    15001 cgccacacct gcgacggagt cattatgccc gtactcaccg aagacgcgcg cgcggtcgcc
    15061 cgcgatcatt atctgctctc catcgccttc gatgccatcg acgccgctcc ggggcagttc
    15121 gtgaacatca aggcaggatc gaatgccgat ccgctcctgc gccgcccatt ctccatctat
    15181 gaccgcgcgg gctccgtgct cgagatcgtg gtccgcgtcg tggggcgcgg taccgcgtgg
    15241 ctctccggat gccgtgcggg cgcggacatc gacgtcctcg cgccgctggg gcgcgggttt
    15301 acgctcgccg aggacaagcg ggcgctcatc gtcggcggcg gcgtggggaa cgcgccgctg
    15361 caatatctgg cgcgggagct tcgcgcgcgc gcatgccgcg tcacgtacct gtacggcgcg
    15421 gcgagcggcg aattcgtgta ttgcgaagac gccttccgcg cggcaagcga cgattttctt
    15481 ctcacaaccg acgacgggag cgcggggcgc cgcgggcgta tcaccgacgc gctcgcggag
    15541 gtcctcgcgg agcgcgcctt cgacatcgcg tacgtgtgcg gaccgacccc gatgatgcgc
    15601 gcggccgcgg ccctcctcgc gccccgcgac atcgccttcg agctgtcggt ggagaattat
    15661 ttcggctgcg ggatcggcgt atgctccggc tgctccatcg gcacgacgga cggcctgcgc
    15721 cgcgcctgcg tggacggtcc cgtcttcgac gggcgcgcga tccactggga atcgctcgag
    15781 gcgcagtcgt gaagccgttc gtcattctga tcgcgttcgc cctgtccggg tgcatcaccg
    15841 cgtcctatca tacggccacc gtcgaattcg gtcacgccta ccgcgaattc gatcgggacc
    15901 accgcgccgc gacgcggatg tacaccggga ctatggcggg caggtgcgat atcaatggcg
    15961 agggcttcca ccgcttccag tttgccggaa tcctgaaagg cgcggggcgc tatctcgatg
    16021 tgctggtgcc ggagcgggaa cgaatgacgc cggcatatga aacgggaccc gacggaattc
    16081 tacgggagtc gaaacgcctc atcaaacgat ccgggctgat tcggatccgc gaaagcgcaa
    16141 cccccgcccc gccgggccct cccgcctatc tcgcatttac gacgatgtcg accatgacca
    16201 tgaagacgtt caccgcgctg atgaaccagg aggttggaaa accatcggat ccgatctcat
    16261 tcctcaatga acacttctcg tttgcggtcg tcagggctga gtatcccctc gccattattc
    16321 atctcgattt ttcgctcaac tacagctata gcgcccgggc ggtcatctgg ggtgcgcctg
    16381 ctgacgggtc gtcgctggtc atctgcaccg gcatcgccgt gggtccctac accgatccgc
    16441 atttcgacat agcatggaaa gagcgaagca ggatcgtata cgccgcgcgc atggctggat
    16501 acgtcggcac cgtgatcgcc gatgtggtga cctcgccggt tcagctggtc atgacgatcc
    16561 ttaccgcgct gacgggtccg ggcgtccggt aacgattgtt acgacagaac cataaaaacg
    16621 tgtacattga cccgctggcg aattgtggta atttttcttg taaatatgcg ctcattattt
    16681 ttcattgttc acatgggatt ggcggcggct tcgtgcgttg ccggaaatcg ctgaggacta
    16741 catgaacgga tttatcatcg caataacggt tctcggggga cttggcctgt tcctcttcgg
    16801 catgaagatc atgagcgagg cccttcagaa ggcgaccggg gacaagctca ggaacatcct
    16861 cacccgggtc accagcaatc gatttttcgg gctcctgacg ggactgggca tcacggccct
    16921 cattcagtct tccagcgcga cgacggtgat gctcgtcagt ttcgtaaacg ccggcctcat
    16981 caacctctcc cagtcggtcg gaatcattct cggcgcaaac atcggtacaa ccgttacggg
    17041 ctggattgtc gcgctcctcg gcttcaaggt tcagatcacc atgttcgccc tgcccgccat
    17101 cgcaatcggc ttcttcctgc gcttcttcaa gaacgagaaa ttcgaggact ggggcgaggt
    17161 catcctcggt ttcgggattc ttttcctggg cctcgacatc atgaattcgg ccgtcaagga
    17221 cctccgcggc tcaagcgcgg tgatgcacct gatgtccatg gtccgcgcgg acaccattcc
    17281 cacggcaatc ctggtggtgg ttatcggcac tctcgtgacc atggtcgtcc agtcgtcgag
    17341 cgccacgatg gccatgacga tggcgctcgc cgtcaacggg atcgtcgatt tttacacctg
    17401 ctgcgcgctc atcctcggcg agaacatcgg caccaccttc accgcttttc tcgcgtccct
    17461 tggcgcatcg acgaccgcac ggcggaccgc gcgggtccat atgctcttca atatattcgg
    17521 ggtcatctgg gtcctgtcct gttttcactg gttgttcatt cccttcatca actgggtggt
    17581 tccgggcgat cccttctcaa cggtagcaag cgaacgctcg aaggtcatcg ccgaccacat
    17641 ggcggcgttt catacgctgt ttaacgtgat caacgcgatg ctgttccttc ctttcacgac
    17701 ggtccttgcg aatatcgcca cgcgccttgt tccggacaag gcgggcaccg ccgagtatga
    17761 cgagtttaat ttgaaatata tcgcgagccc gctcctgtcg acccccgcca tcaacataaa
    17821 ccaggcgcgc atggagacgc aacgcatggt ggggatctcg atcgaaatgt tcgacatggt
    17881 gatggaggtg ttcgggaagc ccggcgaaaa gctgggcgag ctcgtggaaa agatccaggt
    17941 gcgtgagcgc accatcgaca agctggaaat ggagatctcg gagtacttgg tcaagatatc
    18001 gcaggagtca atatcccagg accagagcta cgagatatcg atgctcctgc acgcggtgaa
    18061 cgactgcgag cggatcggag accactgcga gaacctcctg aagctcgtgc ggcgcaagta
    18121 cgacggcaag ctcgagtttt ccgaccgcgc cgtgcaggaa atcacgagca tatccgcaaa
    18181 ggtgcgcgac ctgctcgtgt tgatccatga cggactgatg aaggcgagcg tgagcatcat
    18241 gacgaccgcc agcgtcctcg aaaacaggat caacgagctc aggaaagagc ttcgttccac
    18301 ccatatcagc aggctcaacg agggggcctg cgacgtgtcg tcgggcctgg tgttcatcga
    18361 catgctcacc agcttcgaaa aaatcggcga ccacgcattc aatatcgccg agggcatcgc
    18421 cctgaaaaag ataacctgag ccggcctccc ggcctcctta cgcctccggc gggacgggat
    18481 tatcgtacac ccggttgaga taatcccgca cacatcaatc cagtgcaaga cggcctccct
    18541 cccgatgtgg tactatctgt acacacacat cattttcagg aggtctcaat gaacactttc
    18601 attccacggc ttctctgcgc ctgcgcgttg ctcgtgcccc ttgcggcatt tgcgggcggg
    18661 tccgccgaga agaaatgcaa caccaaatac cccatcatcc tgtcccacgg catgggcgtg
    18721 cgcgacagca tactgggcgt caattactgg tacggcatcc cggacgcgct ccgtgacgag
    18781 ggcgcgcggg tgtacatctc caaccagaac gccttcggat cgcatgccca gcggggcgag
    18841 caactgcgcg cgtacatcat gggcgtgctg gcgctgtaca attacaacac gaaggtgaac
    18901 atcatcgggc actcgcaggg cacgctcgat tcgcgctacg tgatcgcgac ccccatcacc
    18961 tacaccgtcg gcgggaagac ctacacgcgc aagatgagcg attttatcgc gagttggacc
    19021 ggcatcgcgg gccccaaccg cgggagcgcc gtcgccgatg tcattctggg tgcgctgccg
    19081 aacgggacgc atacgacggt cggcgcaatc atcaacgccg ccggatggct catgggcgac
    19141 gtgaaacccg acagcctggg cgcggcgcga aatctcaccc gcgactacat gtgcaatgtc
    19201 ttcaacaagg cctatccgga ccagccgggc gtctattacc agagctgggc ggcgaagata
    19261 aaaacgctca cggccgattg cgtgctgatc gaaccgaccc atttgatcct gaaattttac
    19321 gaaggtgaca atgacgggct ggtatcggtg agctcggcga aatggggcaa ctttcggggc
    19381 gtcgtttccg gcgcctggtg gtgcgggggc gtgtcccact tcaatatcgt cgaccagttc
    19441 ttcggcgtta cgcccggttt cgacgcgccc gcattttatg tcgatatcgt caacgacctg
    19501 aagaaacggg ggtactgaag atatatccga agtcctctcc aggacctctc acggaccgcg
    19561 ctggtatggc cccgcatggg gccaatcttt ttatcgtgag aaacgacaat gggcatggaa
    19621 tctgcccatg cccattgatg ggtatatgag aacccgcccg aatagcgata cgggcgggag
    19681 gagggtgaga tcggtcagtg aaccgcgatt tcgatcttct tcggtttcac ctcttcgcgc
    19741 ttcgccagcg tcagggtgag cacgccgttt tcgatcttcg cgctcacgcc gttgggatcg
    19801 atatcgtcac caaccgtgaa ggtgcgatga tagttgtaca atgcgtattc accgtagcgc
    19861 agcgactcgc gctccttctc gtcggccgtg accgtgccgc ggatgtccag cgtgttttca
    19921 tggagcgtca ccgcgacgtt ttccttcgag accgccggca tatcgaccgt caccacgaag
    19981 ccggtttcat tttcgtagat gtcaacggcc gggacaatct cgcgttcgta tctgttctgt
    20041 tccgcaacat gggtattcgt gctgttcatg gtccacctcc ttactggatc gcaatacgtt
    20101 tcggcttggc cgcctcgctg gcggcgagtg tcacgcgcaa tacgccgtcc ttcagggtcg
    20161 cggtgatgct gtcgcgatcg accgcgatgg gcaggcgaac gctccgggta aactcgccga
    20221 actcgcgttc gcgccggata taggcatgct gctcgttcgc ggcggttcgc gctcccttga
    20281 tcaccagatc gcgatcgacc aactccaggg tgatcgtatc ggccgtgact ccgggaacaa
    20341 gcgcgcgcac ttcaaccgca tcgcccgcat ggtacacgtt cacaacggga tagtttccag
    20401 cgttagtgta ccgatgagtt gtcgcagtaa aaaaatcatc aaaaatgctt ctcagtccaa
    20461 gcatatcggt aaagagatca cttgcagtta tcatgtcatt cctccaatat tgttcatttt
    20521 attgttagca ctttaaataa ttgagtgcta ataacactaa taaaataata cccctcgtca
    20581 gaaacgtcaa ggggtgaatt tgaatttttt ggatttttta cgatcgttct tgtatggtga
    20641 tataaagagt gacagtcccc ctttcgcgcc atacctcgat tttaagggtc tttccaatcg
    20701 gcattttttc gacaatctcg atcagtgcgc cgggtgaatt cacctttgtg ccgtcaacgg
    20761 aaagtatcac gtcgccaatc ctcatgccgg ccttcgcgga tgggcctcca tttagtacgg
    20821 catccacacg cgctccctcc gtgttcttta agccaagctc ttttacatgc tcgtcggtca
    20881 gcggtgcgat atgaaccccg atatacccgc gcttcacctt cttgaatttc ttgaggtcct
    20941 ccaataccgc cttcgcctgg ttgatgggaa tggcgaagcc gatccccagc gacccgccgc
    21001 tgttcgagta gatcatgcga ttgatgccga tcacctcgcc gttgatgttg atgagcggcc
    21061 cgcctgagtt gccggggttg atggaggcgt cggtctggat atgcgtctgg gagccgccca
    21121 tcatgcccac gtccttgcgc gccacggcgc tgatgacgcc gacggtgaag gtcttgtcca
    21181 atccgaaggg gttcccgatc gcgaccgccc agtcaccgac ccgcacggtg tcggaatcgc
    21241 ccagatacac gggattgagt ttcgttttgg gattgatctt caggagcgcg atgtccgtcc
    21301 gcccgtccgc gcccaccacg atcgcgtcat aggtcgtgtc gcccaccttc accttcaccc
    21361 gttccagcgg cccgatgatg ttccccgtgc ggtccatgcg gctcaccacg tgggcgttgg
    21421 tgcagatgta gccgtcgtcc gtgaggacaa acccggtccc cagtcccgtt cgcttctgcg
    21481 tgcgcggctg ctgctgtccc cgcggcatcc cgaagaaatc gcggaaaaac ggatcgttga
    21541 agaacgggtt ctgcggcatc tgcaccgttt gctcggtgct gatgaagacc acccggtcgt
    21601 tgttgagatc gtagatctcg cgaaaggtct tctgcatgtc ccacgcccgc gaggaactcg
    21661 acgagatcgg cgatttcccg tcggcgccga acccgccgcc ggcgcccgtg agccgcgacg
    21721 cggatagcac cacgacacat gcgatggcga caatcacggc aatcgcaatc gcgggcgccg
    21781 atagtatttt ctttcctatg gaatccatac gcacctcata gcaatagatt tgttgtgtgt
    21841 gacgatatca tataataaag aagcggttgg cggtaaaaaa accgcgcaat gatacaggga
    21901 ataccgttct tagacccggc gggcggcgca ttgttacatc gcgttggtgc gggttacggc
    21961 tttgttcctg ctgattgttg tgctgcgcaa aaaatcatgg ccgccgtcaa ttcccgatcg
    22021 atcatcagcc gacgagctcg aggaaaagcc cttcgccgcg ctccagctgg cggacgaggt
    22081 ccgaaaactc ggagtgggtg atgtccttgc ggataatgcg cacgacggag cccgccgcgt
    22141 cgaggagcac gagattgatg aacacctcgc cgccctcctc gaatacatag aaccggtgcg
    22201 gcgaatttcg tgaggccaaa tcctcattca cctgcgccgc catgttccgc aggctctcga
    22261 ggtgcgcgcc cgcctcgtcg ggcgtatcgt ccgtacgcgc atgatcgcgc cctcggcgcc
    22321 gttcgcggtc cggattaatg gtaacggttc tcgcgccggt cgcgtcccag cgattgacac
    22381 ggttcatgcg cgaattctgc tccgtgtcga tgagcatttc cggtcgatgt gaaaacggtg
    22441 ccagcttcat gacatcctcc tcgcttcgcc cgatcaccat atgtatcgtc atttctggcg
    22501 gaaaaatcaa gccaggctaa acatatgttt gtgtttttta tgcaatgccg cagtcgcgcg
    22561 ctatccgcag tgagtgaatt atgtggtgga gtgtgataat gcatccgaac gcccggccgc
    22621 tggatgcgac cggacgttcg aaagcaggga attgcagtac ctagccgaga cgccgttcga
    22681 gcgccgccaa ctcatcagcg agctcgcggg gcagacggtc gccgaaccgc gcataatgct
    22741 cgcgaatgct tgcgatctcg cgcttccatt cgtcgtggtc caccgcgagg agctcccgca
    22801 tgtcggcgtc gctgacgtcg aggccggagc ggtcgatggc gtcaaccgtt ggcatgtggc
    22861 cgatggccgt gtcctgcgcc ttgccctcgc cgttgacccg ctcgaagacc cacttgagca
    22921 cgcggctgtt ttctccgtag ccgggccaga gccatttccc gtcggcggtc ttgcggaacc
    22981 agttcacgta gaatatcttc ggcagttttt cggcgctcgt cctcgagccg atggttatcc
    23041 agtgcttgag ataatccccc atgtgatagc cgcagaaggg gagcatggcc atggggtcgc
    23101 gtcgcacctg gccgatgttc gccgatatcg ccgccgccgt cacctcggag ccgacgatgg
    23161 aaccgaggaa cacgccgtgg ttccaattga atgcctgatg caccagcggg atggtggtgg
    23221 ggcgccgccc gccgaagagg aaggcgctga tcggcacgcc cttcgggtcc tcccagttcg
    23281 ggtcgatcgc ggggcactgc cgcgccggtg ccgtgaagcg cgcattcgcg tgcgcggcga
    23341 gctttccgga gtccttggtc cactctgcgc ccgtccagtc gataatgcgc cctgcgggcg
    23401 gatcataccc gatgccttcc caccagacat cgttgttctc cgtgagggcc acattggtga
    23461 agatggtatt gcggtcgatc gacttcatcg cgttcgggtt cgaatccatc gaggtccccg
    23521 gcgccacgcc gaagaatccg gcctcggggt tgatggcgta aagccgcccg tcctcgccga
    23581 acttcatcca ggcgatatcg tcgcccacgc attccacctt ccagccgggg atggagggaa
    23641 tgagcatggc gagattggtc ttcccgcagg cggaggggaa ggccgcggcg atgtatttct
    23701 tcacgccctg cggattggtg atgcccagga tgagcatgtg ctcggccaac cagccctgct
    23761 cgcgggccat caccgaggcg atgcgcagcg cgaagcactt tttgcccaac agcgcattcc
    23821 cgccatagcc ggatccaaac gaccagatcg tcttctcctc ggggaaatgc gagatgtatt
    23881 tcttttcgat gggcgcgcag ggccacaggt gcccatccga ttcgccggga ccgagcggtt
    23941 tgcccaccga gtgcagacag gggatgaacg tgccgttctc gccgagcgca tcgagcaccg
    24001 cgctcccgac gcgcgtcatg atgtgcatgt tcaccacaac atagggcgag tccgtgagct
    24061 cgatgccgat ctgcgcgatg ggcgagccga ccgggcccat gctgaagggg atcacgtaca
    24121 tagtccggcc cttcatgcag ccggtataga gtcccttcat ggtctttttt aattcatcgg
    24181 ggtcgatcca gttgttcgtg ggaccggcct cgtccttgga tttcgatgcg atgtaggtgc
    24241 ggtcctcgac gcgcgcgacg tccgaggggt ccgagcgaaa gagatatgat ttcgttcgcg
    24301 ttttctcatt gagcggggtc gcgaggccgc aatccaccat gatcttgatc atggcgtcgt
    24361 actccctctg ggacccgtcg caccaataga cgcggtcggg ctggcacatc gcggccatcg
    24421 catcgaccca ttggatgagt ccattgtgtt tcgtcaagct gctgtcgttc atgtgaggca
    24481 cctccctctg tcgtattcgg gcagattcaa ggcgctggca ggagtttcct gatgcctgaa
    24541 aataccaatc gtgtgagtac ccaaaaattc aggtcgttgt ctgtcaattt tctttccggc
    24601 aaatcgatca aaaagagaaa aaatattgct tttccgatgg ccttgcacct aattggtatg
    24661 ctgcccgacc gtgctaaaca ccgcgtcatt acagaatatc cag
//
LOCUS       DAORHF010000032        24057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1348_length_24057_cov_6.013832, whole genome shotgun sequence.
ACCESSION   DAORHF010000032 DAORHF010000000
VERSION     DAORHF010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 24057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1348_length_24057_cov_6.013832"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PKJ16_04670"
     CDS             complement(<1..285)
                     /locus_tag="PKJ16_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46315.1"
                     /translation="MAKDNNKRSDLEVRGMTLKKVLLLAVCFVFIASVPVMAKSFDLV
                     LLHGLTNKHAWSDSFLNTVAATWGSGNVYVIYTNESTRVWTRSINGRTIYF"
     gene            complement(576..995)
                     /locus_tag="PKJ16_04675"
     CDS             complement(576..995)
                     /locus_tag="PKJ16_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46316.1"
                     /translation="MNSTIILRLIAAYNFIMGIIVLFPLLMLAAVSSMMAFSSAIDQQ
                     RHAVSYPSVIIVVLLIVGMIAGIIFLDRGLLRLRNRFRIAEMCLCALQVVMLISMLSK
                     TYGHSVRAIVVSAVFTVIPLSIIAWLAAPSVRSKFTD"
     gene            complement(1015..1614)
                     /locus_tag="PKJ16_04680"
     CDS             complement(1015..1614)
                     /locus_tag="PKJ16_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46317.1"
                     /translation="MKRPRTIVVGALLGLIIVITFLNTGKTPTPDSPDGPRRGDNTAV
                     RDSARDDGSAMSFFSRMFSSDEPGRAPGPGDEEPSGDNEPPRNEIFTLVDQVSKNESL
                     PRNLLFPSGTSKEHIEEHSRNLREMVLYAELIRKGSASPQQKIRYYEIKIKMLEEKRG
                     LIRHYLGLQETDGDREQSEKNLKLLDEKIAVYQEELRAL"
     gene            1803..2330
                     /locus_tag="PKJ16_04685"
     CDS             1803..2330
                     /locus_tag="PKJ16_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018288759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNV46318.1"
                     /translation="MKASVEVLDIKHRIMLIRGERVIIDADLAKVYQVPTKRLNEQVK
                     RNRERFPEDFMFQLTKEEKAEVVANCDHLGTLRFSKSLPFAFTEHGALMAASVINTPR
                     AVQMSVFIVRAFIAVRELLSSRRELDKKLAELERRVGGHDEAIGSLVAAIRRLAGRDD
                     GGAGRSGRKIGFGRE"
     gene            complement(2340..7073)
                     /locus_tag="PKJ16_04690"
     CDS             complement(2340..7073)
                     /locus_tag="PKJ16_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46319.1"
                     /translation="MNLIGLTINTRYRITAQRSENALCVRYAAYDDAGGTPVLVSILK
                     GAALSNRIEDRIRFRHVVEAAAAIDHPNLVAVRETGEVIGLTYLVTELAAGITLDRLL
                     RQGALAPEVGARVGAYVCRALEQLHRAGIVHRDLRPENVLLAAPDASEEARLSDALAT
                     GAPHIARAIAGGFITVKLDGVGLALVREYGGDLSPDEIERTFGYIAPEQTGVLKRPVD
                     ERSDLYSLGVLAYEMLAGSPPFSGGDVCALIHQHVARIPPPPSRVNHAVPAILDAIVL
                     KLIEKEPENRYQTAAGAASDFEAVAGGAADFTPGRHDRSVRLSFRTRIVGREREIAAL
                     KRLFSQAQRGSGCVCLIGGESGSGKTRTVEELRDFVYASGGAMIDGTCAAGAGGRPYG
                     PFREAMDSYLRAYRSYPDARAREIAARLGDEFGELGGIIIQLNPGLTDLLGAASAPVA
                     LKPNREYKRHLMTIARFLFRLSEIENGLTVLIDDLQWADDGTLDILGEIAREIGDHPL
                     LVIGTYRNDEVADDHPLAAFLLRLREAGGPLAEMTLANLDRADMARFVASLLSSPGEP
                     MDRLADFVHARSRGNPFFAIEIVKQLVAEGVLRYGADGWGADPDALVGVAASPSIVDI
                     ILRRIGLLDDIEKNVLSRAAVIGKRFPLDLLYRICALDRSGVVAITDRAAALQLVEPS
                     QQVRGEMHFVHDRVHEAFSVIIDGQTRATLHQRIAEALEEERPDREGPVLFDIVRHYH
                     AAGNADKLLEYAYPAGVRAQGEYANEDAIRYYTMALDILGEQPAPERRADRRRCLERI
                     GEVHLTVGNNDRAIGLYQQILSETDEDAERVRAYKQISQAYFKKGDWRNCERYARRGM
                     RILGENMPVRRAAVIASLVREAALRILHDALPAVFVRSRARDGAERDKLIIWFYVSLG
                     WSFILSDVRKYIHSILRAYNLSESRIGPSAERAMMMTGYATILGAIPLFSRCLRLHER
                     ALRLRMSLGDTWGAAQTHQLTGFAQNWMGDYSRSIQSFERAIELFRRTGDVREIGMST
                     AGLIHNYALIADYASLGDCIERYRDITRSTNDVYGISEADTYRTPYLLETGDIEGALE
                     SGRRAYETSLEKQVYFTHCRACIELGRAHLEQGDPASVDAALEHLSRAASLYHANNFL
                     KQYTVYLFTHLAEALLASFHLTAGTLDPRQRRARLRAIGVACREAERRTRRWVSHHGS
                     ALAACAQHRAACGRTTAAHTLFKAAIAELAKLGRQYELGRAMYHYGIFLMQHGDTARA
                     RELLQAAYQEFMDIGAPRYIARIRTLLGFRDDGGEASSLERFIERERLAAVMRMSQDI
                     AGMADSDELNQAVLARALEITGAQRGLLLLSDGGSGLTVAAGHAGPDGGQPRHSPAIV
                     DEVLARGTYYIGPSDSGSSSVLCAPIRTGSAILGACHLDNPASGDIFTDKEAHLLIAF
                     LTRSAAAIENARLLKRIGPAGPAAEKPGAISQFSEEILKRALDYLADNYLSDISREGL
                     AAHLQVHPDHLGKVFKKGTGKKISEHVNELRIRDAARRLVESDETIIDIAFAVGFESL
                     RTFNRAFQKVMGTTPTEYRETEKKP"
     gene            7300..10131
                     /locus_tag="PKJ16_04695"
     CDS             7300..10131
                     /locus_tag="PKJ16_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HNV46320.1"
                     /translation="MNAGAVSHDSETELDFEGAAPEIAAEAVDGVDAVLRAIADNGFP
                     HDGRFALMRDAHLLRLAYANDKILSLSNSRTQILAHQVESTHRIVNALNRRFLLADEV
                     GLGKTIEGGLVIKELIFRHGYRRILIVCPASLQVQWQSEMRTKFNEEFSIVDGARMRR
                     CARDAAAGGPGPWSALEKAIVSVDFIKNRSFADSLKLNRWDAVIIDEAHRLRRDSLQT
                     TQAYAVAEILSERTECLLLLTATPFRGKLEELYFLVRLIDKNLLGPFQSFYNAYCLGA
                     ADLAELREKIGTVLIRRTKKEIGGFTKRHARTIRFELYPEERALYDATTRYIVEEFNR
                     AVQAENRAVGFVMTVFQKLLDSSSFALMCALRKRRAYLSALVEKGDPRELCAAFLRRP
                     APDAGEEGEEVRDDIFAAVLEKTVAEVRAEIATIDGLIALGAAVTRNKKGDKLRDMIK
                     RSRMLRRNKVLIFTQFRTTQEYLRELLADSDVVVFHGSLSRDEKEEAIRDFRERADIL
                     VCTEAGGEGRNMQFCNILINYDLPWSPLKIEQRIGRIHRFGQKDDVYIFNFSTRDTVA
                     ERVLEVLTRKVKLFEESIGTPDVLLGEIEEELNLGRLFMELVSGRLTRRTVNREIDVR
                     LEAARRGYEKLSDLVVARTMDFNYDEYYRITLKERKFCNRRIEGFVNRAQALDARVRE
                     IIGAKGRLNRLYPVTVAGADGVPVRRMGTFDSARALANEEIEFLAFGNPAVGSIVDHC
                     AGESFGGRSGTVVVEHRIPFIAFYANYAVTFRARSEFQELIPVCVDVFDLLARCDVDA
                     LARELLEAPVADARVPEKYRKYIDHMRAQAEELIDWANGRLRDLAGARAAEIRAGIGG
                     TIDAEIEKIAGAHSRRIKELEEQLERQVGQEKWFDRDMKSAITRTKNKIAAERTEMHD
                     ELARYRAYGAVGFSARLINAGAVLSTAVR"
     gene            10237..12039
                     /locus_tag="PKJ16_04700"
     CDS             10237..12039
                     /locus_tag="PKJ16_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46321.1"
                     /translation="MQRYRHVWSALLFALAIALAAGCSGSTDEPTANTGATLANHIEP
                     AHANEPEDAEDTVGDAPAAAPAIVRKAKAPARPDHYRWTLTFGGRKFDFGRSVAVDSG
                     DHVYLLGSFEDVVDFNPDAEIASPRRSLFGDSVFIVKITAEGRYLWSKALVSTTRGFV
                     KGNHITVDPSDNVYITGSFGGGIYYEGMVDFDPDPEADDLHFASGRGSDAYVTRFNAD
                     GSYGWTRTWGGADGGACGGTSLASDHHNNVYVVGFFSGAVDFGIIDTDGCEKDSCVNV
                     KRSQGAFDVFVTRIDKDGRYAWTRTYGGGATDAAYAIAVDSRDNVYLGGFFQRTVTFN
                     RDWIAEARETGDADGAGAVTRQADAKTSRGGADAFITKIYANGDYAWTRRIGGEYHEA
                     VHAIAVDSRDAVIAVGNFFGAVNFNVVNDPPDMHEGKSVLDLFVTALSAQGEYRWTRV
                     IGGEKSATDDAGVPLYDPDADPGDGYWTNGFYPRAFYSVAVDSRDTIYLAGDFCETVD
                     FDPGPGRDLRESVNRGFDSFVTALAPDGAYRWTRAIQGYDNSYCTGLAVDSRDNLYLA
                     GYFYGRQNFNTDGRRPDSRESAGKSDAFVTSFRK"
     gene            12076..12933
                     /locus_tag="PKJ16_04705"
     CDS             12076..12933
                     /locus_tag="PKJ16_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46322.1"
                     /translation="MGNDTHPITVKFWGTRGSIPTPGKYFVKYGGNTPCVEVRCGDTI
                     LIFDAGTGMRELGASLLHEFAGSPREYHILISHTHWDHIQGFPFFHVAYLPGNKVTFY
                     GGNSVSALENLIFGQMDREYFPVTLFELSAEVTFKTLVESPFAINDVKLYFTHQIHPA
                     LSLGFRIEYRGRVVVYATDSEISNDPEIERINERNIGSLIKDADILIADCQYTAEEYQ
                     AKVGWGHTSIDKAVEMSNRYGVRNLFAFHHDPLRTDEQLDIMIDNARNLVKPPLRLHC
                     AREKTTVRV"
     gene            13146..13718
                     /locus_tag="PKJ16_04710"
     CDS             13146..13718
                     /locus_tag="PKJ16_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46323.1"
                     /translation="MIQLYLAIFFVLILALGALARRSGSSRARAIVGPLAGCAVFLMC
                     LVGFEQYSGVFPILVSAGILLLAIADVYLSIDEADLGRAGRFFALFGSMALLAAFAPH
                     VPFGWWCAPAGAALVALGVWRYRAGAGGVAARARSAAAVMAMSLCALVVAGTAAYYFS
                     WGGESAFLASLVAYFLTVAFRAAGGVIGAK"
     gene            complement(13902..14849)
                     /locus_tag="PKJ16_04715"
     CDS             complement(13902..14849)
                     /locus_tag="PKJ16_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46324.1"
                     /translation="MKVLRLEIGRSFKLENFKKSFKLTWLYSGIGLLIFLVFLSFSGL
                     VQDRSFFYYFFQNAGMFLMFTLLLMAYAGVYRITKFPEDATKVTRTSIRETAKKAHII
                     VGTSITTVIVFFAVVMIEVGISSISMIPYAGPLIIALFTIPIFIINFACLLLAICVFA
                     IVPPLVGEFDSVKEIFTELRHIIRHRWLNVIIYLIISLSILFLSMVVIYFLVRYATGV
                     TKAVQWKINAAYPKLMNALTLKSYFTDLIYKITPQPDPADALKRYGMDLFHYLDILRY
                     IVSISYLAVLSFIASFPLAAYFNISSAFFKSIWKAKKKA"
     gene            complement(14921..15316)
                     /locus_tag="PKJ16_04720"
     CDS             complement(14921..15316)
                     /locus_tag="PKJ16_04720"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV46325.1"
                     /translation="MQEQRQHQRHDIPLQVEFHYFSGDPDRIGDESPAPARGFGTIID
                     ISIGGMSMLSESPVPPGLPISLFFTMLNESGDGARRVLLIANGTVVRSEPQPAAHDGT
                     VTTDEHCRLVAIRFAETQHEIASLFADRR"
     gene            15457..17415
                     /locus_tag="PKJ16_04725"
     CDS             15457..17415
                     /locus_tag="PKJ16_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV46326.1"
                     /translation="MFPKTLVLIIGAAIICAARPDAAAGSPAIVMDARTDRAVVGAHL
                     EILEDPHRALSFEDVRRPEHDRRFRPAPGEAANFGYVSSAFWARFRVEDAAPVPGELY
                     LEMVYTHLDRVTLHLPRPDGSGAVKRGGDLVPYRDREVKYRNTVFILPPERGPRTLYV
                     HVDTESSMQLAMNLWRPRAFAESIKREQYVFGLFYGIMLVMAVYNLFLFLFVRDINYL
                     YYVLYVVAYALVQISYNGIGYEYLWPDQVWWNNRTNPFFIGFSSMCIIQFARSFLGTR
                     ANAPLIDVVLRVSLGLAAAVSVLSLAGGYQASMKAGLALMLVETVIIFVAALMGVAKR
                     YRPARYFLVAWTTFLFGIAAMALKTLGALPDTFITNYSIQIGSALETVLLSVALADRI
                     NTLRDENEKLSVLHKELEIARRIQRSILPEHAPQMPELDIQVRYLPMESVGGDFYDFH
                     VIDRERFGVIIADVSGHGVPAALIAAMLKVAFSMQRDTADDPAALLTSLNATLAGKFS
                     RQFITANYSYLDLSRNVLASSSAGHFPVLVIRRRDGHVHTFEPRGRMMGFVPEINAGT
                     AEIAVGPGDRIMLYTDGVIELRNRAGELFGSERLVALTRELSSADAAGFADAVIDRLT
                     RWRPAGGSFEDDITLIVIDILDGSRKRE"
     gene            17440..22662
                     /locus_tag="PKJ16_04730"
     CDS             17440..22662
                     /locus_tag="PKJ16_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46327.1"
                     /translation="MESIGEYTIIERLDETRGSVVYRAQAPDGAGSVVVKALKTRYPA
                     PAEIARFRQEYDIIRGIESDGIVRALDIVERDDAIAIVLEDFSGISLRRILAEKRLPL
                     RSALLVAAKAANALGDIHRQNIVHKDIKPHNILVNAATGAVKITDFGVAALLTRENEE
                     IYNPLVVQGTLAYLSPEQTGRMNRTVDYRSDLYSLGITLYEMMIGRVPFPSADPMEVI
                     HAHIARRPASPAELRPDVPRTVSDIVMKLLAKTAEERYQNGYGLAADLRECLARLKGT
                     GRIAPFALGHKDISPRFIIPQSLHGREEEVASLLDAFGRVAGGAREVMLVTGDPGIGK
                     SALVHEVHKPIVERRGYFISGKYDQFRRDVPYSALIQSFQGLVRQILTESEERIGRWR
                     DRLGAALGANGRVITDIIPDVELIIGPQPELPALGPDESQNRFNAVFKSFVKAFATEE
                     HPVALFLDDLQWSDAASLGLVATVFADPTLRHFFFIGAYRGGEAAESHLLSLAIETVR
                     SSGVAISSVSLGPLGVDAINELIAHFLRSDIEHTRSLAALVERKTGGNPFFVNQFLKN
                     LYDEGQLLLDPAAGWRWDTDRIEEMQVTENVVDLMSRKIVTLPEETQELLKICACVGN
                     RFDLETLAHIAGVTIDRALADLTVALNEGLVSLHGDLYRFHHDRIQEAAQALIPEDDQ
                     ARWHHAIGAYFLERTAPAELPEKIFYVVNQLNAALPRIVDPAERANLARLNIIAGSRA
                     KSSTAYAEAAGCFRMALRLLPEDAWERDYDTMLHVHRELAASEHLNRNFEAAGALFDT
                     ALRRARSREDRAGLYNLKMIMVANLGRHEEAVDIGLTGLRLLGLRLPKRPGQLSLLMT
                     VARLSCALRSHNAESLISLPPMKDPRVLLMMSVLMNMSSSAYFFSKNLTILIALRMVQ
                     QTLRYGSSPLSAYAFVMYGGVLIAGFQRIDAGHMFGELALAISRAGGAPEQKAKILTI
                     FGLLIEHWRRPLDRNIDVLRESLAVALQTGDHNYACYDMQTIVFTMIARGAPLREIEE
                     ENERFFDFIRQLRDPGALNYLISVRQFVKCLRGETRGPASLADDAFREDEHLFAMLKD
                     NIPIILQRHYLLKLRLQYFAGDYASALAMARASERLLDYSIGQIVTVEHVAFAGLTAA
                     ALADASRGLARIRYRITLWRRRRLLARWAAGCPENFRHKHALLEAEAARLAGNSELAL
                     AAYRKAIREARENGFTQYEAIASERAARFFIGRRNEELARVYLAESIRAYEAWGATAK
                     ARALEVEFAAELERMSRAQRDFGFDGTSTGTSSGGSLRALDLQAVMKASQAISSQLEL
                     ARILEVIMKISIENAGAERGLLILQKESDGRLYVEAEGSVEGGIVVLQSVPIDLEHAT
                     TDTSAAEGGASILPSGIINYVNKTHESVVLNNASTDGMFIGDPYVVSRNTRSVLCGPV
                     VHQGKLSGILYLENNLTDGAFTPDRLELLRILSGQAAISIENARLLEHRENAAKLGRD
                     MEIAANIQTALLPAAPAIPGYDLCAYLKPAEAVGGDYYDVICEGGRHWMVIGDVSGHG
                     VHAGLIMMMAQTAIHVALNERPELTPAELLSTVNTTLTRNIQRLGEYKYMTASAFAVG
                     ADGTVDYAGLHQDILIYRARDGSVETIQTKGIFVGITEEIADKLEDERFTLEPGDTML
                     VFTDGITEMWRKGSVRGKRTPERDMFGIERLRNLFQSLAGSAAVEDIEAGILGSLDEY
                     EPRDDITMMIVRRR"
     gene            23335..23853
                     /locus_tag="PKJ16_04735"
     CDS             23335..23853
                     /locus_tag="PKJ16_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV46328.1"
                     /translation="MFLKNREFAEAYNDLYPVVFGAVYSKVGNPEDAQDICQELFVKL
                     YEKLETIENRRKWLYGALRLEVMAYYRKKKPDGVDIDEVFADVGLTFVNGFRDARIVI
                     SGAIEHDENFEDERERALFDLVAVQNYSYEQAGMQLGMSKRQVRYRYGLIVERILAYL
                     QKQGIKSLEDLL"
     gene            23850..>24057
                     /locus_tag="PKJ16_04740"
     CDS             23850..>24057
                     /locus_tag="PKJ16_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46329.1"
                     /translation="MNGSVSVFGRAMERFRFVEPVPPEVQRHIRRVKRREFLKILRRA
                     GAYSTLFAIVSSVFFTLKKLGVSIT"
BASE COUNT         4312 a         7499 c         7943 g         4303 t
ORIGIN      
        1 gaaatagatg gtgcggccat tgatcgagcg ggtccatacc cgggtcgatt cattcgtgta
       61 aatcacatat acattgcccg atccccacgt cgcggccact gtgttaagga aggaatcgct
      121 ccacgcatgc ttgttggtca aaccatgcag cagcaccaga tcgaacgatt tcgccatgac
      181 gggaaccgag gcgatgaaaa cgaaacaaac cgcgagaaga agaacttttt taagtgtcat
      241 acccctgacc tccaggtcgc ttcttttatt gttgtcctta gccatttttt attctggaat
      301 cccttgtttc ttcaacaatc catccagacg gtttcttgaa ccagtcataa tatgcagaat
      361 taatgatcat acgtcttgcc aatgctagaa cgatctgttt ttgtccaggg ggtgtggcgt
      421 ttgattaggc gggtgggctc ggatttgatc cagaatcgat aggattatta ctgccaaatc
      481 atgctatcgg cacgcaaaaa tagacaaaat ccgtcttttg gttattagaa agacgaaagt
      541 atacactgcc tgttcaggtt gatccagacg caggcttaat cggtaaactt gctcctcacc
      601 gagggggccg caagccaggc gatgatggag agggggatca cagtgaatac tgcggatacg
      661 acgatagcgc gaacgctgtg cccgtatgtc ttcgagagca tgctgatgag catgacgacc
      721 tgaagggcgc agaggcacat ctccgcgatc cgaaaacggt tgcgcagccg cagaaggccc
      781 cggtcgagga agatgatacc ggcgatcatc ccgacaatca ggagcacgac gatgatgact
      841 gaggggtaac tgacggcgtg gcgttgctga tcgatggccg agctgaaggc catcatggac
      901 gagacggcgg cgagcatgag cagcgggaac agcacgatga tacccatgat aaaattgtac
      961 gcagcgatga gtcgaagaat gatggtgctg ttcatgtcgc gcctcctctc cgggctagag
     1021 cgcccgtaat tcttcctgat acacggcgat tttttcatca agcaatttta ggtttttttc
     1081 actctgttcc cggtcgccgt ccgtttcctg caggcccagg tagtgccgga taagcccgcg
     1141 cttttcctcc agcatcttga tctttatctc gtagtatcgg atcttctgtt gcgggctcgc
     1201 gctccccttt cggatgagct ccgcgtagag caccatctcg cgcaggttgc gcgagtgctc
     1261 ctcgatatgc tccttcgatg tccccgacgg aaacaaaaga ttgcgcggaa ggctttcgtt
     1321 cttcgatacc tggtcgacga gcgtgaagat ctcgttacgc ggcggctcat tgtcaccgga
     1381 gggctcctcg tcgccgggac cgggcgcgcg gccgggctcg tcggacgaga acatgcggct
     1441 gaaaaaactc atggcggaac cgtcgtcccg cgcggaatcg cgcacggcgg tattgtcgcc
     1501 acggcgcggt ccgtcagggg aatcgggtgt gggcgttttc ccggtattga ggaaggtgat
     1561 gacgatgatc agcccgagca gggcgccgac gacgatggtg cggggacgct tcattacgca
     1621 atccaatacg gtaaaatgcg tgatgtcaag ttattaaaaa aacatctacc atggaatgac
     1681 gaacccgtgc acgcaccctc tcgaacaatc ggacgatccg tcgctgtgaa aaagtgacat
     1741 gtttcaatgc cggaatcgta tatatcgtag tacgtgattc tatcgttcga atgaggatgc
     1801 gcatgaaggc gagtgtggaa gtgctggaca tcaaacacag gattatgctg attcgcggcg
     1861 agcgggtcat catcgacgcc gacctggcta aggtctatca ggtgcccacg aaacgattaa
     1921 acgaacaggt caagaggaat cgggagcgat ttcccgagga cttcatgttt cagctcacca
     1981 aggaagaaaa ggccgaggtg gtcgcaaatt gcgaccacct cggaacgctc agattttcta
     2041 aatcgcttcc gttcgccttt accgagcacg gggcgctcat ggcggccagc gtcatcaaca
     2101 ctccccgtgc cgtgcagatg agcgtgttta tcgttcgtgc gttcattgcc gttcgcgagc
     2161 tcctttcgtc gcgacgcgag ctggacaaaa agctcgccga gctggagcgg cgggtgggcg
     2221 gccacgacga ggcgatcggg tcgctggtcg ctgcgattcg ccggcttgcg ggcagggacg
     2281 atgggggcgc gggaaggtcc ggcaggaaga tcggatttgg ccgcgaatga cgtgcggcct
     2341 tacggttttt tttctgtctc gcggtattcc gtgggcgtgg tgcccatcac cttctggaag
     2401 gcgcggttga aggtgcgcag gctctcgaag cccacggcaa aggctatgtc gatgatggtc
     2461 tcgtcgctct cgacgaggcg gcgcgccgcg tcccggatgc gcagctcgtt tacgtgttcg
     2521 ctgatcttct tcccggtccc ttttttgaac accttgccca ggtggtcggg gtgcacctgg
     2581 aggtgcgcgg cgagaccctc gcgcgatatg tccgatagat agttatccgc cagatagtcc
     2641 agcgcgcgct tgaggatttc ctcggagaac tgggagatgg cgcccggctt ctccgccgcg
     2701 ggaccggccg gtccgatgcg cttgaggagc cgggcgtttt cgattgccgc ggcgcttcgg
     2761 gtcagaaagg cgatcaggag gtgcgcctcc ttgtcggtga agatgtcgcc gcttgcggga
     2821 ttgtcgagat gacaggcgcc gaggatcgcg ctcccggttc ggatcggcgc gcagagcacc
     2881 gagctcgatc cggaatcgga cgggccgatg taataggtgc cgcgcgcgag cacctcgtcg
     2941 acgatcgcgg gggaatggcg cggttgcccg ccgtcgggcc cggcgtggcc ggcggcgacg
     3001 gtgaggccgc tcccgccgtc ggagagaagc agcagcccgc gctgggcgcc ggtgatctcg
     3061 agggcgcgcg cgaggacggc ctggttgagc tcatcgctgt cggccatgcc ggcgatgtcc
     3121 tggctcatgc gcatgaccgc cgccaggcgc tcgcgctcga taaagcgctc gagcgaggac
     3181 gcctcgcccc cgtcgtcgcg aaatcccagc agggtccgga tgcgcgcaat atatcgcggg
     3241 gcgccgatgt ccatgaactc ctgataggca gcctggagca gttcccgcgc acgcgccgtg
     3301 tcgccgtgct gcatgaggaa gatgccgtag tggtacatcg cgcgccccag ctcgtactgt
     3361 cgccccagtt tcgccagctc cgcgattgcg gccttgaaga gcgtatgcgc cgccgtggtc
     3421 cgcccgcacg cggcgcggtg ttgcgcgcag gccgccagcg cggagccgtg atgcgacacc
     3481 cagcgccgtg tcctgcgctc ggcctcgcgg caggcgacgc cgatcgcgcg caggcgcgcc
     3541 cggcgttggc gcggatcgag cgtccccgcc gttaggtgaa acgatgcgag gagcgcctcc
     3601 gccaggtgcg tgaacaggta gaccgtgtac tgcttgagga agttgttggc atggtagagc
     3661 gatgcggcgc gcgagagatg ctccagcgcc gcgtccacgg acgcgggatc gccctgctcg
     3721 agatgggcgc gcccgagttc aatgcacgcg cggcagtgcg taaagtacac ctgtttctcg
     3781 aggctggtct cgtaggcgcg ccggccgctc tcgagcgcgc cctcgatgtc gccggtctcc
     3841 agaagatagg gggtgcgata cgtgtcggcc tcgctgatgc cgtagacgtc gttggtggac
     3901 cgggtgatgt cgcgatagcg ctcgatgcag tccccgagcg acgcgtagtc ggcgatgagc
     3961 gcgtagttgt ggatgaggcc ggccgtggac atgccgatct cgcggacgtc gcccgtgcgg
     4021 cggaacagct cgatggcgcg ctcgaaggac tggatgctgc gggagtagtc gcccatccag
     4081 ttttgcgcaa agcccgtgag ctgatgggtc tgcgcggccc cccaggtgtc gcccagcgac
     4141 atgcgcaatc gcagcgcccg ctcgtgcagg cgcaggcacc gggagaacag ggggatggcg
     4201 cccaggatgg tcgcgtagcc ggtcatcatc atcgcgcgct ccgcggaggg gccgatgcgg
     4261 ctctccgaga gattgtaggc ccgcaggatg gaatgaatgt acttgcgcac gtcgctgaga
     4321 atgaaggacc agccgagaga gacgtagaac cagatgatga gcttgtcgcg ctccgcgcca
     4381 tcgcgcgcgc gcgaacggac gaacaccgcg gggagcgcgt cgtgcaggat gcgcagggcc
     4441 gcctcgcgga cgaggcttgc gatcaccgcc gcgcggcgta ccggcatgtt ctcgcccaga
     4501 atgcgcatgc cccgccgggc gtaccgctcg cagtttcgcc agtcgccctt tttgaagtag
     4561 gcctggctga tctgcttgta cgcacgcacc cgctccgcat cctcgtcggt ctcggagagg
     4621 atctgctggt agagcccgat cgcccggtca ttgttgccaa ccgtgagatg cacctcgccg
     4681 atccgctcga ggcaccggcg ccggtcggcc cgacgctcgg gcgcgggctg ttcgcccagg
     4741 atgtccaatg ccatggtgta atagcgtatg gcgtcttcgt tggcgtattc cccctgcgcg
     4801 cggacgccgg cgggataggc gtattcgagc agcttgtcgg cgtttccggc ggcgtgatag
     4861 tggcggacga tgtcgaagag cacgggcccc tcgcgatcgg ggcgctcctc ctcgagggcc
     4921 tcggcgatcc gctggtgaag cgttgcgcgc gtctggccgt cgatgatgac cgagaaggcc
     4981 tcgtgcaccc ggtcgtggac gaagtgcatc tctccgcgca cctgctggct cggctcgacc
     5041 agctgcaggg cggcggcgcg gtccgtaatg gccaccacgc ccgaccggtc cagcgcgcag
     5101 atccgataga ggaggtccag cgggaagcgc ttgccgatca ccgccgcgcg cgagagcacg
     5161 ttcttctcga tgtcgtccag gagcccgatg cgccggagga tgatatcgac gatggacgga
     5221 gaggccgcaa cgccgacgag cgcgtcggga tcggcccccc agccgtccgc gccgtaacgg
     5281 agcacgccct ccgcgacgag ctgcttgacg atctcgatgg cgaagaaggg gttcccgcgg
     5341 cttcgagcgt gtacgaagtc cgcaagccgg tccatgggct ccccggggga ggacagcagc
     5401 gaggcgacga agcgggccat gtcggcgcgg tcgaggttcg ccagcgtcat ctccgcgagc
     5461 ggtccgccgg cctcgcgcag gcgcagaaga aatgcggcca gcggatggtc gtcggccacc
     5521 tcgtcgttgc gataggtgcc gatgacgagc agcggatggt ccccgatctc gcgggcgatc
     5581 tcgccgagta tgtcgagcgt cccgtcgtcg gcccactgga gatcgtcgat gagcaccgtc
     5641 agtccgttct cgatctcgct cagacggaac aggaatcgcg cgatggtcat cagatggcgc
     5701 ttgtattcgc ggttgggctt gagcgcgacc ggcgcggatg cggcgccgag gagatcggtg
     5761 aggccggggt tcaactggat gatgatgccg cccagctcgc cgaattcgtc cccgagccgc
     5821 gcggcgatct cgcgcgcccg cgcgtccgga tagcttcgat aggcgcgcag atacgagtcc
     5881 attgcctcgc gaaaggggcc atagggacgg ccgccggcgc cggcggcgca ggtgccgtcg
     5941 atcatcgcgc cgccgcttgc gtacacgaaa tcgcgcagct cctccaccgt gcgcgttttc
     6001 ccggaaccgg actcgccgcc gatgaggcag acgcacccgc tcccccgctg cgcctggcta
     6061 aagagccgct tgagcgcggc gatctcgcgc tcccgcccca cgatgcgcgt gcggaagctc
     6121 aggcgcacgc tgcggtcgtg ccgtcccggc gtgaaatcgg ccgcgccgcc ggcgacggcc
     6181 tcgaagtcgg acgcggcgcc cgcggccgtc tggtagcggt tctccggctc cttctcgatg
     6241 agcttcagga cgatggcgtc gaggatcgcg ggcacggcgt ggttgacgcg cgaggggggc
     6301 ggcggaattc gcgcaacgtg ctggtggatg agcgcgcaca cgtcgccgcc ggaaaacggg
     6361 ggcgatccgg cgagcatctc gtacgcgagc acgcccagcg aatagaggtc gctgcgctcg
     6421 tcgaccggtc gcttgaggac gccggtctgc tcaggagcga tgtagccgaa ggtccgctcg
     6481 atctcgtcgg gggacaggtc gccgccatac tcgcgcacga gggccagtcc cacgccgtcg
     6541 agttttacgg tgatgaatcc gccggcgatc gcccgcgcaa tgtgcggcgc gcccgtcgca
     6601 agcgcgtcgg acaggcgggc ctcctcggag gcatcgggcg cggccagcag gacgttttcc
     6661 ggccgcaggt cgcggtggac gatgcccgcg cggtgcagct gctccagcgc ccggcacaca
     6721 tacgccccca cgcgcgcccc gacctcgggc gcaagggccc cctgtctgag gagccggtcg
     6781 agcgtgatgc ccgcggcgag ctcggtcacc aggtaggtga ggccgatcac ctcgccggtt
     6841 tcccgcaccg cgacgaggtt ggggtggtcg atggcggcgg ccgcttcgac aacgtggcgg
     6901 aagcggatgc ggtcctcgat gcggttcgag agggcggcgc ccttgaggat ggaaacgagc
     6961 actggcgtcc cgcccgcgtc gtcgtaggcc gcatagcgga cgcacagcgc gttttcggag
     7021 cgctgtgcgg tgatgcggta gcgggtgttg atggtcaggc cgataaggtt catggcgcgc
     7081 cggcgacgat gccgggatgc gcgcgatggt cgcacggctc gtggccccgt gtcaaataca
     7141 atcgcgggaa aaaaaacttg cattttctct ccgggggaat attatgtcac gtatacacgg
     7201 gtcaatcaaa cctgcgcgtt cggaatactt cattataaaa aaccaggacc ggcgtccgtt
     7261 ccgggagccg gtcgatgatg tgcggggtgg ggaattgttg tgaatgccgg tgccgtatca
     7321 cacgatagcg agacggagct cgacttcgag ggagcggccc cggagatcgc ggcggaggcc
     7381 gtcgacggcg tcgatgcggt gcttcgcgcg atcgcggaca acggtttccc gcacgacgga
     7441 cgcttcgcgc tcatgcgcga tgcgcatctg cttcggctcg cctatgcgaa cgacaagatc
     7501 ctgtcgctct cgaactcccg cacgcagatc ctcgcgcacc aggtcgagtc cacgcaccgc
     7561 atcgtgaacg cgctcaatcg gcgcttcctt ctcgccgacg aggtgggcct gggcaagacc
     7621 atcgagggcg ggctcgtcat caaggagctc attttccgtc acggctaccg gcgcatcctc
     7681 atcgtgtgcc cggcgtcgct ccaggtgcag tggcaaagcg agatgcgcac caagttcaac
     7741 gaggaattct ccattgtcga tggcgcgcgg atgcgccgat gcgcgcggga tgccgccgcc
     7801 ggcgggcccg gtccctggag cgcgctcgag aaggcgatcg tctcggtcga tttcatcaag
     7861 aaccggtcgt tcgccgacag cctcaagctc aatcgctggg acgccgtgat catcgacgag
     7921 gcgcaccgcc tgcgccgcga ttccctgcaa accacccagg cctatgcggt ggccgagatc
     7981 ctctccgagc ggacggagtg tctgctgctg ctcaccgcga ccccgttccg gggaaagctc
     8041 gaggagctgt atttcctggt gcgcctcatc gataaaaact tgctggggcc gttccagtcg
     8101 ttctacaacg cctactgcct gggcgccgcc gaccttgcgg agctgcgcga gaagatcggc
     8161 acggtgctca tccggcgcac caagaaggag atcggcggct tcacgaagcg ccacgcgcgc
     8221 accatccgct tcgagctcta cccggaggag cgggcgctct acgacgccac cacgcgctac
     8281 atcgtcgagg agttcaaccg cgccgtgcag gcggagaacc gcgcggtggg cttcgtgatg
     8341 accgtcttcc agaagctgct ggactcctcg tcgttcgcgc tcatgtgcgc cctgcgcaag
     8401 cggcgcgcct acctgtccgc gctcgtggag aagggcgacc cgcgcgagct ctgcgccgcc
     8461 tttctgcgcc ggcccgcgcc ggacgcgggc gaggagggcg aggaggtgcg cgatgacatc
     8521 ttcgcggcgg tgctggagaa gaccgtcgcg gaggtgcgcg cggagatcgc aaccatcgac
     8581 ggcctgatcg cgctgggcgc cgccgttacg cgaaacaaaa agggcgacaa gctgcgcgac
     8641 atgatcaagc gctcgcggat gctgcgccgc aacaaggtgc tcatcttcac gcagttccgc
     8701 accacgcaag agtatctccg tgagctgttg gccgacagcg atgtcgtcgt gttccacggc
     8761 tcgctgtcgc gcgacgaaaa ggaggaggcg atccgcgact ttcgcgagcg cgccgacatc
     8821 ctggtgtgca cggaggcggg cggcgagggc cgcaacatgc agttctgcaa cattcttatc
     8881 aactacgacc tgccgtggtc gcccctgaag atcgagcagc gcatcggccg catccaccgc
     8941 ttcggccaga aggacgacgt ctacatcttc aacttctcca cgcgcgacac cgtggccgag
     9001 cgcgtgctgg aggtcctcac gcgcaaggtc aagctcttcg aggagtccat cggcacgccc
     9061 gacgtgcttc tgggcgagat cgaggaggag ctcaatctgg ggcgcctctt catggaactg
     9121 gtgagcgggc gcctcacccg gcgcacggtg aaccgggaga tcgacgtccg cctggaggcc
     9181 gcgcgccggg gctacgagaa gctctcggac ctggtggtgg cgcgcaccat ggacttcaac
     9241 tacgacgagt actaccgcat caccctcaag gagcgcaagt tctgcaatcg ccgcatcgag
     9301 gggttcgtga accgcgcgca ggcgctcgac gcgcgcgtcc gcgagatcat cggtgcgaag
     9361 gggcggctca accggctcta tccggtgacc gtggccggag ccgacggcgt cccggtgcgg
     9421 cggatgggca ccttcgacag cgcccgcgcg ctcgcgaacg aggaaatcga gtttttggcc
     9481 ttcggcaatc ccgcggtggg gagcatcgtc gatcactgcg cgggcgaatc attcggcggc
     9541 cgttcgggga ccgtggtggt ggagcaccgc atccccttca tcgcctttta cgcgaactac
     9601 gcggtcacct tccgcgcgcg cagcgagttt caggagctca ttcccgtgtg cgtggacgtc
     9661 ttcgatctgc ttgcgcgctg cgatgtggac gcactggcgc gagaactgct cgaggcgccg
     9721 gttgcggacg cgcgggtgcc ggagaagtat cggaagtaca tcgaccacat gcgcgcgcag
     9781 gcggaggagc tcatcgattg ggccaatggc cggctgcgag atctggccgg cgcgcgcgcc
     9841 gccgagatcc gcgccgggat cggcgggacg atcgacgccg agatcgaaaa gatagccggc
     9901 gcgcactccc gccgcatcaa ggagctcgag gaacagcttg agcgccaggt gggccaggag
     9961 aagtggttcg accgcgacat gaagagcgcc atcacccgca ccaaaaacaa gatcgccgcc
    10021 gagcggaccg agatgcacga cgagctcgcg cgctatcgcg cctacggcgc ggtgggtttc
    10081 tcggcgcgcc tcatcaacgc aggggcggtc ctgtccacgg ccgtgcgcta gccgcgcctc
    10141 ccgtttttat cttgccaaaa cacatggccc cgtatacggt ttgcccgtga caggcccggc
    10201 ggacgtttcg tgccgggttg tgcggggggg atgatgatgc aacgataccg acatgtgtgg
    10261 agcgcccttc tgttcgcgct cgcaatcgcg cttgccgcgg gctgctccgg atcgaccgac
    10321 gaaccgacgg cgaacaccgg cgccacgctc gcgaaccaca tcgagcccgc ccacgcgaac
    10381 gagccggagg acgccgagga caccgtcggc gacgcgcccg ccgccgcgcc cgcgatcgta
    10441 cggaaggcga aggcgccggc ccggcccgac cactatcgct ggaccctcac cttcggcgga
    10501 cggaaattcg atttcggccg gtccgtcgcc gtcgattcgg gcgatcacgt ctacctgctg
    10561 ggaagcttcg aggacgtggt cgatttcaat cccgacgccg agatcgccag cccgcgccgc
    10621 tcgctcttcg gcgactcggt cttcatcgta aagatcaccg cggaggggcg ctacctctgg
    10681 tccaaggcgc tcgtctcgac gacgcgcggc ttcgtcaagg gaaatcacat aacggtcgat
    10741 ccgagcgaca acgtgtacat caccgggagt ttcggcggcg gcatctacta tgagggcatg
    10801 gtggacttcg atcccgatcc cgaggccgac gatctccact tcgcctcggg gcgcgggagc
    10861 gacgcgtacg tgacccggtt caacgccgac gggagctacg gctggacgcg cacctggggc
    10921 ggcgccgacg gcggcgcctg cggcgggacc tcgctggcga gcgatcacca caacaacgtg
    10981 tacgtggtgg gatttttcag cggcgccgtc gatttcggca tcatcgacac ggacggatgc
    11041 gagaaggaca gctgcgtgaa cgtgaagcgc tcccagggcg cgttcgacgt attcgtgacg
    11101 cgcatcgaca aggacgggcg ctacgcctgg acgcgcacct atggcggcgg cgccacggac
    11161 gccgcatatg cgatcgccgt ggattcgcgg gacaatgtct atctcggcgg cttcttccag
    11221 aggacggtca ccttcaatcg ggattggatc gccgaggcgc gcgagacggg cgacgcggac
    11281 ggggccggcg cggtcacgcg gcaggcggac gcgaagacct cgcgcggcgg tgccgacgca
    11341 ttcatcacca agatatacgc gaacggggac tatgcctgga cgcgccggat cggcggggag
    11401 tatcacgagg cggtccacgc catcgcggtc gattcgcgcg atgccgtgat cgccgtcggc
    11461 aactttttcg gcgcggtgaa cttcaacgtc gtgaatgatc cccccgacat gcatgaggga
    11521 aagagcgtgc tcgacctgtt cgtgaccgcg ctctccgcgc agggcgagta tcgctggacg
    11581 cgcgtcatcg gcggcgagaa gtcggcgacg gacgatgccg gagttccgtt atacgatccc
    11641 gacgccgatc cgggcgacgg ctactggacg aatgggttct acccgcgcgc cttctactcg
    11701 gtggcggtgg actcgcgcga cacgatctac ctcgccggcg atttctgcga gacggtcgat
    11761 ttcgatcccg gtcccgggcg cgatctgcgc gagtccgtga accgcggctt cgattcgttc
    11821 gtgaccgcgc tcgcgcccga cggcgcctac cgctggacgc gcgccatcca gggttacgac
    11881 aactcgtact gcacggggct ggcggtcgac tcccgcgaca atctctatct tgccgggtat
    11941 ttctacgggc ggcagaattt caacacggac gggcggcgcc ccgattcacg ggagtcggcc
    12001 gggaagagcg acgcgttcgt gacctcgttc cggaaataaa atcggcgcgc ccgtacgcgg
    12061 gcgacggtgt gattcatggg aaacgatacg catcccatca cggtgaaatt ctggggcacc
    12121 cgcggctcca tccccacgcc ggggaaatac ttcgtgaaat acggcgggaa cactccctgt
    12181 gtcgaggtgc ggtgcgggga caccatcctc atcttcgacg ccgggacggg catgcgcgag
    12241 ctgggcgcct cgctgttgca tgaattcgcc ggaagcccgc gcgagtatca catcctcatc
    12301 agccatacgc actgggacca catccagggc ttcccgtttt ttcacgtggc ctatctgccg
    12361 ggaaacaagg tcacctttta cggcggcaac agcgtctcgg cgctcgagaa cctgatcttc
    12421 gggcagatgg accgcgagta cttccccgtg accctcttcg agctctccgc cgaggtcacc
    12481 ttcaagaccc ttgtggagag tcccttcgcc atcaacgacg tgaagctgta ctttacgcac
    12541 cagatccacc cggcgctgtc gctcggattt cggatcgagt accgcggccg ggtcgtggtg
    12601 tacgcgaccg acagcgagat atccaacgat cccgagatcg agcgcatcaa cgagcggaac
    12661 atcgggagcc tcatcaagga cgccgacatc ctcatcgccg actgccagta caccgcggag
    12721 gagtaccagg cgaaggtcgg ctgggggcac acctcgatcg acaaggccgt ggagatgagc
    12781 aatcgctatg gcgtaaggaa cctcttcgcc ttccatcacg atccgctccg caccgacgag
    12841 cagctcgaca tcatgatcga caatgcgcga aacctcgtga agccgccgct tcggctccac
    12901 tgcgcgcggg agaaaacgac tgttcgtgtg taagatgcgg cgcgtgaccg cgcgtgatcg
    12961 gcatgcgagg ccgtgaaaaa atttatacat tccccggcgg cgcgcgtaat atctatgtga
    13021 acgacactaa cgatgggcgc gcggccgttc tcggggccac cgcggattgc cgccgcgccc
    13081 cgttcacttt tttctcttga ttctctcccg ccggtgtgca caattgcttc accgggagga
    13141 gactcgtgat ccagctctat cttgccatct tcttcgtgct gatcctcgcg cttggcgcgc
    13201 tcgcgcgccg ttccggaagc tcccgggcgc gcgccatcgt ggggccgctc gccggctgtg
    13261 cggtgtttct gatgtgcctg gtgggcttcg agcaatactc cggggtgttt cccatcctgg
    13321 tgtcggcggg cattctcctg ctcgcgattg ccgatgtcta cctttcgatc gatgaggcgg
    13381 acctggggcg ggccgggcga tttttcgccc tctttggcag catggcgctt cttgcggcct
    13441 tcgcgccgca tgtcccgttc gggtggtggt gcgcgccggc gggcgcggcg ctcgtcgcgc
    13501 tgggggtgtg gcgctatcgt gcgggggcgg gcggtgtcgc ggcgcgggcg cgcagcgctg
    13561 cagccgtcat ggcgatgtcg ctgtgcgcgc tcgtcgtcgc cggcacggcc gcgtattact
    13621 tctcgtgggg cggggaatcg gccttccttg cgtcgctggt cgcgtatttc ctcaccgtcg
    13681 cgttccgcgc ggccggcggc gttatcggcg cgaaataatc atcgaaatga actagtacgg
    13741 tatcgcggga tcccctgttt gcgcgggtgg tgaacttgcg ttatttgcgt gtatttcgcg
    13801 aacgacaact cgacgcatcc gcacacggcg aagcgatcat gatgcgcgcg cggtgcgagt
    13861 ctctactgtc gatgcgttaa tcctgcggct gaccgatggt attacgcctt ctttttcgcc
    13921 ttccagatgc tcttgaaaaa cgccgaggaa atattgaaat acgccgccag cgggaacgag
    13981 gcgatgaacg agagcaccgc gagatacgag atgctcacga tgtagcgcag gatgtcgaga
    14041 tagtggaaca ggtccatccc gtagcgcttg agcgcgtcgg ccgggtcggg ctgcggggtg
    14101 atcttgtaga tgaggtcggt gaaatacgac ttgagcgtga gcgcgttcat gagcttgggg
    14161 taggccgcgt tgatcttcca ctgcaccgcc ttggtgacgc ccgtcgcgta gcggaccagg
    14221 aaatagatca cgaccatgct caggaataat atcgagagcg agatgatgag atagatgatg
    14281 acgttgagcc accggtgccg gatgatgtgg cgcagctccg tgaagatctc cttgacggaa
    14341 tcgaactcgc ccacgagcgg cggaacgatg gcgaagacgc aaatggccag caggagacag
    14401 gcgaaattga tgatgaagat gggaatggtg aatagcgcga tgatcaacgg ccccgcgtag
    14461 gggatcatgg agatcgacga gattcccacc tcgatcatca ccactgcgaa aaaaacgata
    14521 accgtcgtga tggaggtccc gacgatgatg tgcgccttct tggccgtctc cctgatggag
    14581 gtccgggtca ccttggtggc gtcctcgggg aacttggtga tgcggtacac gccggcatag
    14641 gccatgagca aaagcgtgaa catgaggaac atgcccgcat tctggaagaa gtaatagaag
    14701 aagcttctgt cttgaaccag tcccgaaaac gagaggaaga caaggaagat gagcaggccg
    14761 atgccgctgt agagccaggt gagcttgaag ctcttcttga agttttcgag cttgaagctc
    14821 ctgccgatct cgagacgcaa taccttcatg ggcgacccct gccgcgtgga atgcacgcac
    14881 actgacggcc gccgacgaaa aaatcaacaa aaaaacgccg ttaccgccgg tccgcgaaca
    14941 acgatgcgat ctcgtgctgg gtctcggcga aacggatggc gacgagccgg cagtgctcgt
    15001 ccgtggtcac ggttccgtca tgtgcggccg gttggggctc gctgcggacc accgtcccgt
    15061 tggcgatcaa gaggacgcgc cgtgcgccgt ccccggactc gttgagcatg gtgaaaaaca
    15121 gcgatatggg aaggcccgga gggacgggcg attccgacag catggacatg ccgccgatgc
    15181 tgatgtcgat gatggtgcca aagccgcgcg cgggcgccgg gctttcgtcg ccaatcctgt
    15241 cgggatcccc gctgaaatag tggaactcga cctgcagggg gatgtcatgt cgctggtgct
    15301 gtcgctgttc ttgcatgggc gtcctccggt gtccagcgcc ggcctgccgg ccgatcacgc
    15361 taccatgggc cgcgcgcggt ttcaagaaat aaaatatttt tcattgcatt ttacccggcc
    15421 acatgcggtg ctctgtatga ggatatcgag aacgccgtgt ttcccaagac cctcgtactg
    15481 atcatcggtg ccgcgatcat ctgcgcggcg cgacccgacg cagccgccgg ctcgccggcg
    15541 atcgtcatgg acgcgcggac ggatcgtgcc gtcgtgggcg cccatctcga gatcctcgaa
    15601 gacccccacc gcgccctctc gttcgaagac gtccgccgcc ccgagcacga ccgccgcttc
    15661 cgccccgcgc ccggcgaggc ggcgaatttc ggatacgtgt cgtcggcctt ctgggcgcgc
    15721 tttcgcgttg aggacgccgc gccagtaccg ggcgagctct acctcgagat ggtctacacg
    15781 cacctcgacc gggtcacgct ccatctgccc cgcccggacg ggtccggcgc cgtcaagcgg
    15841 ggcggcgatc tcgtacccta ccgcgaccgc gaggtgaaat atcgcaatac ggtgttcatc
    15901 cttccccccg agcgggggcc gcggacgctg tacgtgcatg tcgacaccga aagctccatg
    15961 cagcttgcga tgaacctgtg gcggccccgc gccttcgcgg agtccatcaa gcgcgagcag
    16021 tacgtgttcg gcctgttcta cgggataatg ctggtcatgg cggtgtacaa cctgttcctc
    16081 ttcctgttcg tgcgcgacat caactatctc tattacgtgc tgtacgtcgt cgcctacgcc
    16141 ttggttcaga tatcgtacaa cgggatcggc tacgagtatc tctggcccga ccaggtctgg
    16201 tggaacaacc gcaccaaccc gttcttcatc ggcttctcgt cgatgtgcat catccagttc
    16261 gcccggagct tcctggggac gcgggcgaac gcgccgctga tcgacgtcgt gctgcgggtg
    16321 tcgctgggcc tcgccgcggc ggtgtcggtc ctgtcgctcg cgggaggata ccaggcgagc
    16381 atgaaggcgg ggctggcgct catgctcgtc gagaccgtca tcattttcgt ggcggccctc
    16441 atgggggtgg cgaagcgcta tcgcccggcg aggtatttcc ttgtcgcctg gaccaccttc
    16501 ctgttcggga tcgcggcgat ggcgctcaag accctggggg cgcttcccga cacctttatc
    16561 accaattact cgatccagat cggctccgcg ctcgagacgg tgctcctctc ggtcgcgctc
    16621 gcggaccgga tcaacaccct gcgggacgaa aacgagaagc tgagcgtgct gcacaaggaa
    16681 ctggagatcg cgcgccgcat ccagcgctcg atccttccgg agcatgcccc gcagatgccg
    16741 gagctggaca tccaggtgcg ctatctcccc atggagtcgg tggggggcga tttctacgat
    16801 ttccacgtga tcgatcgcga gcgcttcggg gtgatcatcg ccgatgtctc gggccacggg
    16861 gtccccgcgg cgctcatcgc cgcgatgctc aaggtcgcct tttccatgca acgggacacg
    16921 gcggacgacc cggcggcgct gctgacttcg ctcaacgcga ccctggccgg gaagttcagc
    16981 cggcagttca tcaccgccaa ctattcgtac ctcgacctct cgcgcaacgt cctcgcgagc
    17041 tcgagcgccg ggcacttccc ggtcctcgtg atccgccgtc gcgacgggca tgtccacacg
    17101 ttcgaacccc gcgggcgcat gatggggttc gtgccggaga tcaacgccgg gaccgcggag
    17161 atcgccgtgg ggcccggcga ccggatcatg ctgtacaccg acggcgtcat cgagctgcgc
    17221 aatcgcgcgg gcgagctctt cgggagcgag cgcctcgtcg cgctgacccg cgagctgtcg
    17281 tcggccgacg cggccgggtt cgccgacgcc gtcatcgacc gactgacccg gtggcggccg
    17341 gcggggggga gtttcgagga cgatatcacc ctcattgtta ttgacatcct ggacggctcc
    17401 cggaagcgtg agtaggggcg gccgtccgcc tggcgcgtca tggaaagcat cggcgaatac
    17461 accatcatcg aacggctgga cgagacgagg ggatcggtcg tctaccgcgc ccaggcgccc
    17521 gatggggcgg ggagcgtcgt ggtcaaggcg ctcaagacgc ggtatcccgc cccagccgag
    17581 atcgcgcgct tccggcagga gtacgacatc atccgcggca tcgagagcga cggcatcgtc
    17641 cgggcgttgg acatcgtcga gcgcgacgac gcgatcgcca tcgtcctgga ggacttctcg
    17701 gggatatcgc tccggcggat cctcgccgag aagcggctcc ccctgcgctc ggcgctgctc
    17761 gtcgcggcga aggccgcaaa cgcgttgggc gacatccatc gacagaacat cgtccacaag
    17821 gacatcaaac cgcacaatat cctggtgaac gcggcgacgg gcgcggtgaa gatcaccgat
    17881 ttcggcgtgg ccgcgctgct cacgcgcgag aacgaggaga tctacaaccc gctggtggtg
    17941 caggggacgc tcgcctatct ctctcccgag cagaccggcc gcatgaaccg gacggtggac
    18001 taccgcagcg acctctattc gctgggaata acgttgtacg aaatgatgat cgggcgcgtg
    18061 cccttcccct cggcggaccc catggaggtg atccacgcgc acattgcgcg ccgtcccgcg
    18121 tccccggcgg agctccggcc cgatgtgccg cggacggtga gcgacatcgt gatgaagctc
    18181 ctggccaaaa cggccgagga gcgctaccag aacggctacg gccttgcggc cgacctgcgt
    18241 gagtgcctcg cgcggctcaa aggcaccggg agaatcgcgc cctttgcgct ggggcataag
    18301 gacatctcgc cgcgcttcat catcccccag agcctgcacg ggcgcgaaga ggaggtcgcc
    18361 tcgctcctgg acgccttcgg gcgcgtggcc ggcggcgcgc gcgaggtgat gctggtgacc
    18421 ggcgatccgg ggatcggcaa gtcggcgctg gtgcatgagg tccacaagcc catcgtggag
    18481 cgccgcgggt atttcatctc cgggaagtac gaccagttcc gccgtgacgt gccctacagc
    18541 gcgctgatcc agtcctttca ggggctcgtg cggcagatcc tcaccgagag cgaggagcgg
    18601 atcgggcggt ggcgcgaccg gctgggcgcg gcccttggcg cgaacgggcg ggtgatcacc
    18661 gatatcatcc ccgacgtaga gctcatcatc gggccgcagc cggagcttcc cgcgctgggt
    18721 ccggacgaat cgcagaaccg cttcaacgcg gtgttcaaat ccttcgtgaa ggccttcgcg
    18781 accgaggagc acccggtggc gctctttctg gacgacctgc aatggtccga tgccgcgagc
    18841 ctcggcctgg tcgcgacggt tttcgccgat ccgaccctgc gccacttttt cttcatcggc
    18901 gcctaccgcg gcggcgaggc cgcggaatca cacctgctgt cgctcgcgat cgagaccgtc
    18961 cgctcctcgg gcgtggcgat ctcgtcggtg agcctggggc ccctgggcgt ggacgccatc
    19021 aacgaactca tcgcgcattt tctgcgcagc gacatcgagc acacgcgttc gctcgccgcg
    19081 ctggtcgagc gcaagaccgg cggaaatccc ttcttcgtga accagttcct gaagaacctc
    19141 tacgacgagg ggcagctcct cctcgacccg gcggcgggat ggcgctggga cacggaccgc
    19201 atcgaggaga tgcaggtgac cgagaacgtg gtcgacctca tgagccgcaa gatcgtgacc
    19261 ctcccggagg agacgcagga gctgctcaag atctgcgcct gcgtgggcaa ccggttcgat
    19321 ctcgagacgc tcgcccacat cgccggcgtg acgatcgacc gggccctcgc cgacctgacg
    19381 gtggcgctca acgagggact ggtgagcctg catggcgacc tgtaccgctt ccatcatgat
    19441 cgcattcagg aggccgcgca ggccctcatt cccgaggacg atcaggcgcg ctggcaccac
    19501 gccatcggcg cctatttcct cgagcgcacc gcgccggccg agctccccga aaagatcttc
    19561 tacgtggtca atcagctcaa tgcggcgctt ccgcgcattg tcgatccggc cgagcgcgca
    19621 aatctcgcgc ggctcaacat catcgcgggc tcgcgcgcga aatcgtccac cgcctacgcc
    19681 gaggcggccg gctgcttccg catggcgctt cgtctcctgc ccgaggacgc gtgggaacgc
    19741 gactacgaca cgatgctgca cgtccaccgc gagctcgccg cgagcgagca cctgaaccgg
    19801 aacttcgagg ccgccggggc gctcttcgac accgcgctgc gccgcgcccg ttcgcgcgaa
    19861 gaccgcgcgg gtctgtacaa tctcaagatg atcatggtgg cgaacctcgg ccgacacgag
    19921 gaggcggtcg acatcggcct caccggcctt cggttgctcg ggttgcggct tcccaaacgc
    19981 ccgggacagc tttcgctgct gatgacggtc gcgcgtctct cgtgtgcgct ccgctcgcac
    20041 aatgcggaat cgctcatatc gctgcccccg atgaaggacc cgcgcgtcct gctcatgatg
    20101 agcgttctga tgaacatgtc cagctcggca tatttcttca gcaagaatct caccatcctc
    20161 atcgcgctca ggatggtgca gcagacactc cgctacgggt cgtcgccgct ctcggcctac
    20221 gccttcgtca tgtacggcgg cgttctcatc gcgggcttcc agcggatcga tgcggggcac
    20281 atgttcgggg agctcgccct cgcgataagc cgcgccgggg gcgcgccgga acaaaaggcg
    20341 aaaatcctca ccattttcgg cctgctgatc gagcactggc ggcgcccgct ggaccgcaac
    20401 atcgatgtgc tccgcgaatc gctcgccgtg gcgctccaga ccggcgacca caactacgca
    20461 tgctacgaca tgcagacgat cgtcttcacg atgatcgcgc ggggcgcgcc cctccgggag
    20521 atcgaggagg agaacgagcg ctttttcgac ttcatccggc agctcaggga ccccggcgcg
    20581 ctcaactacc tgatctcggt gcggcagttc gtcaagtgcc tgcgcggcga gaccagaggg
    20641 ccggcgtcgc ttgccgacga cgccttccgc gaggacgagc acctgttcgc catgctcaag
    20701 gacaatatcc ccatcatcct gcagcggcac tacctgctga agctgcggtt gcagtatttc
    20761 gcgggggatt acgcgagcgc cctcgcgatg gcgcgcgcct ccgaacggct gctggactac
    20821 tcgatcggcc agatcgtgac ggtggagcac gtcgccttcg cgggtcttac ggcggcggcc
    20881 ctggccgacg cctcgcgcgg gctggcgcgc attcgatatc gcataacgct ttggcgccgg
    20941 cggcgcctac ttgcgcgctg ggcggcgggc tgcccggaaa acttccgcca caaacacgcg
    21001 ctccttgagg ctgaggcggc gcggctcgcg ggaaacagcg agctggccct cgcggcctac
    21061 cggaaggcca tccgggaggc ccgcgagaac ggcttcacgc agtacgaggc catcgcgagc
    21121 gagcgcgccg cgcggttttt catcggccgt cgcaacgaag agctcgcgcg ggtctacctg
    21181 gccgagtcga tccgcgccta cgaggcctgg ggcgccaccg cgaaggcgcg ggccctggag
    21241 gtcgagttcg cggcggagct ggagcgaatg tcgcgcgcgc agcgtgattt cggcttcgac
    21301 ggcacctcga cggggacctc gtccgggggc agtctgcgcg cgctcgatct tcaggccgtc
    21361 atgaaggcct cgcaggccat ctcgagccag ctcgaactcg cgcgcatcct cgaggtcatc
    21421 atgaagatct cgatcgaaaa cgccggagcg gagcgggggc tgctcattct ccagaaggag
    21481 agcgacggac ggttgtatgt ggaggccgag gggagcgtcg agggcggtat cgtggtcctg
    21541 cagtcggtgc cgatcgatct ggagcacgcg acaaccgaca cgtcggcggc cgaagggggc
    21601 gcgtcaatac tgccctcggg gatcatcaac tatgtgaaca agacgcacga gagcgtcgta
    21661 ctcaacaatg cctcgaccga cggaatgttt atcggcgatc cgtatgtcgt ctcacggaac
    21721 acccggtcgg tgctctgcgg acccgttgtc caccagggga agctctccgg gattctctac
    21781 ctggagaaca atctcaccga cggggccttc acgccggacc ggctggaact cctgcggatc
    21841 ctctccgggc aggcggccat ctccatcgag aacgcgcggc tcctggagca ccgcgagaac
    21901 gccgcgaagc tggggcgcga catggagatc gcggccaata tccagacggc gctgttgccc
    21961 gcggcgcccg ccattcccgg ctacgacctg tgcgcctatc tcaagccggc cgaggccgtg
    22021 gggggcgatt actacgacgt tatctgcgag ggagggcggc actggatggt catcggcgac
    22081 gtctcggggc acggggtgca cgccggactc atcatgatga tggcgcagac cgcgatacac
    22141 gtcgcgctca acgagcgtcc cgagctcacg ccggcggagc ttctgtcgac ggtgaacacg
    22201 acgctgacca ggaacatcca gcgtctcggc gagtataaat acatgaccgc aagcgccttc
    22261 gccgtgggcg cggacgggac ggtcgactat gccgggctcc accaggacat cctgatatac
    22321 cgcgcccggg acgggtcggt ggagaccatc cagacgaagg gcatcttcgt gggtatcacc
    22381 gaggagatcg cggacaagct cgaggacgag cgcttcacgc tcgaaccggg cgataccatg
    22441 ctcgtcttca ccgacgggat caccgagatg tggcgaaagg ggagcgtgcg cggcaagcga
    22501 acgcccgaga gggacatgtt cgggatcgag cgcctgcgga acctcttcca gtcgctggcc
    22561 gggagcgccg cggtcgagga catcgaggcg ggaatcctcg gctcgctcga tgaatacgag
    22621 ccccgcgacg atatcaccat gatgatcgtg cggaggcgct agggcgcgcc atcattcacc
    22681 tttccggaaa atattctttc aatctcatcc atgcgttgac agcgaaggat tattatcctt
    22741 cagcaggcgg catgaacacg ggccgctttc tttcttcgca gaaagaaagc ggccaaagaa
    22801 atgcgccact caggcgccgt ggcggcatga ctttcggagc gtggggccat ccggagcgcg
    22861 cagcccatgt aacccgacat cctgaagggc tccctggtgc aatgttcaga aggcgaactc
    22921 gtaaaatgtc aatagcatac gcaacaggcc ctcgagcgga agtataagcg cagtggggct
    22981 cgagggaggc gaagcaaata gattcgggaa atatctgtag tttaaccggg cggagccact
    23041 aagccgctcg cggcggtgag cgagcgcgga agaggagagc gcgagaggag aaggccgcga
    23101 aggccttttc ctctcgcgga tttggatttg actgcgaacg aggtgctgat cgcctggcga
    23161 gtgcaccaga aaaaggccaa tccgtcatcg gcctcgagtg aaacatcccc acgcggcata
    23221 aaattcttga cgcatggcgt ctatacggcg gaattattgg ccaaataaaa tgaaatattt
    23281 tgtcacaatg cgggcgatta tgacgttact attccagacg gcgcaggatg cgccatgttc
    23341 ctgaagaacc gggaattcgc ggaggcgtat aacgacctct atccggtcgt gttcggcgcg
    23401 gtgtactcga aggtggggaa tcccgaggac gcgcaggaca tctgccagga gctgttcgtg
    23461 aagctctacg agaagcttga gacgatcgag aaccggcgga agtggctcta tggggcgctg
    23521 cggctcgagg tgatggctta ctatcggaag aagaagcccg acggcgtcga catcgacgag
    23581 gtcttcgccg acgtggggct caccttcgtg aacgggtttc gcgatgcgcg catcgtcatc
    23641 agcggggcga tcgaacacga cgagaacttc gaggacgagc gcgagcgcgc gctcttcgat
    23701 cttgtcgcgg tgcagaacta ctcctacgag caggcgggga tgcagctggg catgtccaag
    23761 cgccaggtgc gctaccgcta cgggctcatc gtggagcgta tcctggcgta tttgcagaaa
    23821 cagggcatta agagcctgga ggacttgcta tgaatggaag cgtttcggtt ttcggcaggg
    23881 cgatggagcg cttccgtttc gtggagcccg tcccgcccga ggtgcagcgt catatccgcc
    23941 gagtgaagcg gcgcgagttc ctgaagattc tcaggcgcgc gggggcctat agcacgctct
    24001 ttgcgatcgt cagctcggtt tttttcacgc taaaaaaact gggagtatcg atcacca
//
LOCUS       DAORHF010000033        23533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1399_length_23533_cov_5.633572, whole genome shotgun sequence.
ACCESSION   DAORHF010000033 DAORHF010000000
VERSION     DAORHF010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23533
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1399_length_23533_cov_5.633572"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..502)
                     /locus_tag="PKJ16_04745"
     CDS             complement(44..502)
                     /locus_tag="PKJ16_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46330.1"
                     /translation="MIPKNNILTNKFVELIEENHQQITEYYMNDLLGDPDTTAYRTLD
                     RQVIYESGNQIYRELSSYITKDFSKDEIAKHYKKLAWERFEKGVPASHVFKALMLLKR
                     HVWLFIQKKMGDDPTDFRQALELSNRVVLYFDRAAHAVLKGYEDIDKKRW"
     gene            complement(628..1671)
                     /locus_tag="PKJ16_04750"
     CDS             complement(628..1671)
                     /locus_tag="PKJ16_04750"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HNV46331.1"
                     /translation="MRMRRVLYGFAVMAGVALCTGGSAPPDARTGDATGWWRVFFTAP
                     GSPRNDRDGPEAAFIRLLGAAERTIHGAFYDISSPEIARAFISAAARGVSVRLVTDDG
                     NAGNDEIARIRSAGIPVVADERRGFMHNKFAIIDGEIVWTGSFNLTRNCARKNNNNAI
                     MIRSPELARIYEAEFDEMFTHRVFGNRAEPGPFAVVRNRYYVRIGESHINVHFSPENG
                     VERIIAKRIDKAKKSICFMLFSFTSDPIGEAMIAKFKSGVPVHGIFERRGAGSASSEY
                     TKMRTEGLPVMLDRNPRAMHHKVLIIDERLVITGSYNFSKNADRRNDENIIIIDNPEI
                     AAEYLAEYRRLAK"
     gene            complement(1830..2795)
                     /locus_tag="PKJ16_04755"
     CDS             complement(1830..2795)
                     /locus_tag="PKJ16_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46332.1"
                     /translation="MTDYIEHKTPKGILIKLYPAKREFLAFDRNGSSLYADGHNLRLM
                     HKAPSVFFLGERFHVKNTAGEDLSFVKISRGKLVIPYEFQDEKRYVFIARDEALDVLP
                     VVVKCLRNFNLLANEVKIGRAPDYIIVDTAITRNDIIVIKNLCTAVQVIITGDKPIDA
                     DAPPQPGEGIDTTRATELIKDVNINMLSQNPVFLARVHLRRMDLAKVKQLLLDFDLAE
                     IDAQYILNFVVTMIGKADRDPDLRRERATLELLSNSFRFYLNLLMKDDDAVRRMIQAE
                     VNQNELVSYHTLIAKTKLLFPSSDDQLRLTEIENLIWEAMEKLKK"
     gene            2915..6262
                     /locus_tag="PKJ16_04760"
     CDS             2915..6262
                     /locus_tag="PKJ16_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HNV46333.1"
                     /translation="MHSINVSRRAVHAGAIADSSGELDAKYSVKNIVRNEGSWRPAAR
                     PGAAYDFFTVDFGAEVPVNFVEIAPYPPARGAFPAAFRIEASDDNASWRVIHAEKNFA
                     LDADEASFLLSFPLATLRYLKVVIYHSGQEKTDAPEIARLSAGIAGVKTIVGPSSSEA
                     HAPATLVSPAPGAYWESAINQNRATAVLDIDLGTSCNLAAMTLTAHGGSPCAFPEHFL
                     LELSADNKLWTTIREEKNFAAEPKAKYRWDFPVTPARYARITATDVKLSQKHFGVRLA
                     GVELFAVLPNTGHTHNISGLTLPASVFNPGIVRLARDGETAQGAAVQGNDSRLRDAST
                     IFKGIVQLADHGSTEDLRAVQASDPRLAPATDGRHGTVRLAYDREVNPNAVVRSDDSR
                     LSEATDSSYGIVKLCPDGVYSKMTVVTGNDSRLKSATTSSFGIVRLADDGETAQDCVI
                     TGADRRLRDASTLYRGIVELAENGEDAPNVAVQGNDKRLKDATTATKGIVELAENGED
                     APNVAVQGNDKRLKDATTATKGIVELAENGEDAPNVAVQGNDKRLKDASTTAKGIVEL
                     AENGEDAPNVAVQGNDRRLKDATESRPGILRFAKNLESAPLCAVQGNDHRLRDATTAY
                     RGIVELAENGEDAPGVAVQGNDKRLKDASTTAKGIVELAEDGEDKAGIAVQGHDRRLK
                     NATETTTGIVRFAGNGESASNAAVQGNDKRLKDATTTAKGIVELAENGEDAPNVAVQG
                     NDKRLKDASTTAKGIVELAENGEDAPSVAVQGNDKRLKPASVGAPGIVSLAAHGETRE
                     GRVVQANDPRLSDARTPLPHDHDYARRRHDFSEHRGTIAIADKKNEPFIGLTPPPEGA
                     GIISAKNITIDDGGVGVAGVALHPGESYGKSYGVVGHAQFVGMRGQSTGSAEASARGC
                     GVMGLSRFGAGGVFASEHGYSLVVEGFGDIADYDDSARLAGDGRALYLRGSSDINGRM
                     SIINTQSGAPGNIVEMYEVDDVDYLSPGNLLVAGAAGGVLSRSRSRYDRAVIGIVAGN
                     PLIILNGKGAKDKFYPVVLGGKAVCTVDARERPIKPGDLIVSSDTPGCGMAGTIDSFE
                     KIGTVIGKALEGLESGMGSIMVYVVHL"
     gene            complement(6286..6942)
                     /locus_tag="PKJ16_04765"
     CDS             complement(6286..6942)
                     /locus_tag="PKJ16_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46334.1"
                     /translation="MNKKTRRGLLCLVALCALGCASTVRVHPRVTFPLALTEGAPVLI
                     FPINLDYTGAPIDSRKSWPTLASGIAARLGKNAVWDGRLYDMAGNLSFELPEAMDAAM
                     RAGQWRLDGGRERIAVALEVVVRRMTKSLVASRQVPRDFMFTHVLVVHSRGRVTLGGT
                     AISHESWGGVYRVADRTIVSFVRTPMTIAHAKGEMPARLSELYARMAADLIAGKPTTR
                     "
     gene            complement(7040..7663)
                     /locus_tag="PKJ16_04770"
     CDS             complement(7040..7663)
                     /locus_tag="PKJ16_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46335.1"
                     /translation="MNKKITIAVLICMALGTALYAQTRQSGRAAEDKSAKTAKNIPDG
                     YGSLKWGALTAASKTGVLGKIVYTDEKKVIISRDGEIEYLYGFFYVDPAVAAAPEAKD
                     AKDAKSDGRLYYVVVSFPYLALDDVRKKVEAKFGVPTGENIKKNQGAYVWEADKTTVI
                     LWVDEYEKKPYTRKLTYVGKDIAKEINDYQLKVFTAGERKVLEGLTP"
     gene            8131..8565
                     /locus_tag="PKJ16_04775"
     CDS             8131..8565
                     /locus_tag="PKJ16_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46336.1"
                     /translation="MLVRIRYTIVVDGEKIPCGSAQYAYGAGNTLRRITYTALSDTVR
                     RRTYPSGVQMFEYSGGRVAARRIIEYAYNRKINAVMQIGQHAISYHNAAPALLQSWVL
                     DPETGVIARTRETNGTALRERITRIETALAAAASGVHFTCTN"
     gene            8574..8888
                     /gene="trxA"
                     /locus_tag="PKJ16_04780"
     CDS             8574..8888
                     /gene="trxA"
                     /locus_tag="PKJ16_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HNV46337.1"
                     /translation="MAGLIEATDANFKTEVLDASGTVLVDFWAPWCGPCRMQTPILEK
                     LAGSGEIGAKIVKLNTDDNPNTAQTYGIQSIPTLIIFKNGAETERMGGVQPEKALKDK
                     LK"
     gene            8899..9348
                     /gene="dtd"
                     /locus_tag="PKJ16_04785"
     CDS             8899..9348
                     /gene="dtd"
                     /locus_tag="PKJ16_04785"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HNV46338.1"
                     /translation="MRAVLQRVLSASVTINGEVVSAIGPGMLALVGFRRGDTDDDMAY
                     LIDKTLGVRIFDDERGVMNRSLAEIDGELLVVSQFTLYGDARKGRRPSYGEAMPPDEA
                     RVLYDAFLRRCRERHPRVCGGVFGADMKVALVNDGPATILLDSSRNL"
     gene            9449..9907
                     /locus_tag="PKJ16_04790"
     CDS             9449..9907
                     /locus_tag="PKJ16_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46339.1"
                     /translation="MPIDTTLYIHENTLKLISESCDMSGISHNRLIVMLLKNAMSKAR
                     KNMKTGLAIRYQERNQGGHWHTVHVRFREDEADFFKDMRNFMKMSVSLILAQAAREYL
                     KEMVRASAERETTDNYPFSNHQLSFIETNDGIFWITHWKTPPPPSPTIKH"
     gene            complement(9897..10967)
                     /locus_tag="PKJ16_04795"
     CDS             complement(9897..10967)
                     /locus_tag="PKJ16_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HNV46340.1"
                     /translation="MKIGIIGLPQTGKKSFFELLTGAKAPSAPQDQMKALSGIAEVRD
                     ERFDRLAALYRPKKETRARIAVDLVPRIDPGSVRPEVLAIAAETDALCHVVRAFEDDA
                     VYHVSGSVNPARDIALVNDELVLHDQLFIEKRLERIAKNQKKGRDERSAREEALLASF
                     REHLDAGRPLRTRAISDEEAALIASYPFLTMKKMIVAVNAGDADIASGCISAAVEEAL
                     TESGIACMRISAKLEAEIALLADEAERREFMAASGIAAPALNVLTGLCMDALGLISFF
                     TVGTDEVRQWEVRRGAAAPEAAGAIHSDIQRGFIRAEVMKYPDLIEAGSEDAVKKAGR
                     FHVMGREYIVEDGDIISFRFNV"
     gene            complement(11065..14892)
                     /locus_tag="PKJ16_04800"
     CDS             complement(11065..14892)
                     /locus_tag="PKJ16_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46341.1"
                     /translation="MAINVSDSNPPRPRILLVEDEPIVLLDVTHILTDLGYEVAGTAT
                     TGEQAVSLAAALAPDLIIMDIVLQGAASGIDAARAIRSNAPIPIIYMTANADPATIDA
                     ARDTGPSGYVTKPVSSTDLHSNIESALYKDRMERRLRESEARFRTLFHFAGEGILVAE
                     VESKRFTYANPAICKMLGYTEEELLRLSVGDIHPPEAVASVVREFMSQARGDTILAAD
                     IPCLRKDGTVIYADINTSAMMIDGRECNVGFFTDITARKDIERALRDNEEKYRRLIET
                     TDTGFVIVDGRGHVVDANDIYVKMTGHATLEQIRGRNVLEWTAAHDRERNDAAVAQCA
                     ALGFIRGFEVEYEWPNGVIIPIEINATVTGSGDAARILTLCRDISERVGAARALRESE
                     ERFRLAAQATGQLVYDYDIPAGVINWAGAIAAITGFTPEEMRAVDINEWEEMIHPDDR
                     ARATTALERSINTGTLYSIEYRFRVKGGSYMDIEDVGAFIPDRNGVPYRMVGTMKDIS
                     ERKRAARELAETTKRLESILSITRTGVDIIDADYNLRYVDPGWQKVYGDPAGRKCYEY
                     FMGRQRPCSDCGIPAAITSRQITITEEVLPREGNRAIEVHTIPFQNPDGEWLVAEFNI
                     DITERKKAETALRASEQKFITAFNSTPVLMAISTIDTGQFFEVNQSFLDVLGFSREEV
                     IGKTSRDLGLFDDVSRRDEIMSALERDGFARDFDVQVRTRSGVLRNGSFSADIVELEG
                     ERYLLTAMNDITERKKAEEALERERNQFVSGPMVAFKWRAEPGWPEEYVSPNVSRWGY
                     DPASFIEGRTSYLDIIHPDDRERIEREVARYGEAGTPSYEQEYRVFTASGEVRWVHDF
                     TVVIRDAAGAVTHYDGYLSDITERKKAEEDRARLEDQLRQSQKIESIGRLAGGVAHDF
                     NNLLTAITGNISLALMDVPAGDALAETLKDAMKAAESAAALTRQLLAFSRKQIVEPRV
                     VGVNDLVSNMKKMLGRIIGEDIALKTVLAPDAGAVRIDPGQFEQIIINLTVNARDAMP
                     GGGELIIETATLDLDEEYASGHPYVSPGPYVLISVSDTGSGMSSEVKEHLYEPFYTTK
                     PKGKGTGLGLATIYGAVKQNSGSIEVYSEVGLGSTFKIFFPRVADGSEATLPGRLVGD
                     FPRGTETLLLVEDDPAVRGLAVRLLSRLGYRLMVFGNPMEALHAVEAHAGAIDLMVTD
                     VVMPGMNGLELAQRVGKLRPGLRILFSSGYSEEVIAHHGIIDRGLNFIAKPYRPQDLA
                     KKIREVIDGPAPA"
     gene            15006..15683
                     /gene="radC"
                     /locus_tag="PKJ16_04805"
     CDS             15006..15683
                     /gene="radC"
                     /locus_tag="PKJ16_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HNV46342.1"
                     /translation="MAHRETPVTDTLPVQRCRDGADIRELADHELVAIIIGTGTRGTG
                     VVRMGMEILERFGGVRGIRCAGLRELANAKGMGMVKAARLQAALELGRRAMATVEPGA
                     SVSSPRRVWELLRHEIAGVPQEVFRVLVLNSKNLLVKNRVVSVGTISAALIHPREIYR
                     DAIRETGSSIIIAHNHPSGVLTPSGEDIAATKRIADAGNIIGIALLDHVIIAEAGYLS
                     LREAGYM"
     gene            complement(15634..16887)
                     /locus_tag="PKJ16_04810"
     CDS             complement(15634..16887)
                     /locus_tag="PKJ16_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNV46343.1"
                     /translation="MLLPRIVPRARAAMFLAGALALSCNAVKKEPAVVVPVYPEKLAI
                     TKAESLRTGIGAKSVEMSPDGSRVYSMNLEECSVYEYNRASRVIQRKLIFVMTPGKGW
                     DYDKKVPINSYQEKPVEGHFSHNGRYLWVSLHNADGVAVWDLLGGDTAVEGKPFKTAW
                     LHERIPPKPADVSAPPAAKKKAAPEPEFTNKKMKLLLIKTGATPKVIWSSPNGKYLFV
                     SNWHSSTVSVIDISAPTPDGWNVVKTLRTGSVPRGMTASADSKFLYIAQMGGNFLSVV
                     DLDTVEKVREITVGPAPRHILISNGYLYASICNEAKLIKVELPSGRIVQWARTMSSPR
                     TIAASPDGTVIFVTCYNDNTVQAFRADTLALLATRESPGRPVGITVWQGGGVYEAWIC
                     NHTNFVMNVFTYTPPPAGSGTPPRQ"
     gene            complement(17033..18139)
                     /locus_tag="PKJ16_04815"
     CDS             complement(17033..18139)
                     /locus_tag="PKJ16_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46344.1"
                     /translation="MKPADIAAHAGALRDAVRRCRHLLIYIKGSPDPDVIASSFAVHA
                     LCAAEGVRATIVSITVVSLPQNEAMIRDLRIPIAFSRELPRLSSYDAYAVMDFQSAHI
                     KGVSERLRCAIHIDHHDAVAEDLPVDFKLVSGAAGSVSTLVALMLEALAPPFDAPLMR
                     RVATALTYGIQTDTDNYAHANDLDRRALAYLARHADPEEIRHIARIPLSEEVVRLMAL
                     AERERVVYKGWALAGIGFIDESRRDGIAIIADSMARDPEIAVVVVFAVVVREATRGLA
                     LNASIRAKDGSLDLTELIRRITPEGGGRAFKGAYQVNLDYFFSCPDRARLWEVVRATT
                     LEALTQQRDAVGLIELKGVYKRIRRRLGNLWGGS"
     gene            complement(18136..20310)
                     /locus_tag="PKJ16_04820"
     CDS             complement(18136..20310)
                     /locus_tag="PKJ16_04820"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46345.1"
                     /translation="MPMTLSPLLTLEIVLTGCAAVLAGYFALVNIVRRSRFVINRVFA
                     LISFITLCLYLSLGALLVAPGFEYRTELGRLFFLLILGATQLFFHLTEIFPRWEKRSP
                     GWFIALSALPGLVLAGALLFTDAIVVETVQQDHLAFRFGRFFIAYIALFGLYIFGSFL
                     ILLYKSSFLRNQSFRVQIFYMFMGTNLGSLLILVFTFMLPIMFNVHAYKNIAIAVSGS
                     LLLLVINYALSDERMLDFKRFYLRGIYWVTVFALLVGPVYAGVEFGMGRVVFGQTVPA
                     PALAFGVFLYLFVFYRSARPRLERFFQSEYRAFERNVLEFFQELSGLAGAGERGYVEF
                     FNNAIDMLEHRFGIARATFLTRDRAGDAFEFSYSLGERISLSPLPADGELARALVEYA
                     GLIDRSMIFTDERLAEKREELIALFDRHDIQVAVPLFDYEKRLIGVILTGSLVSGRPY
                     NINLLQALELYRIQFELTLANAIMLDEVKRTQAASHDRLVVRTIKGRIIPRTLAQIDG
                     IRLSSLYKNVSERGGDYFDAVAINPDTLGIFIADTADLGVESGLLGLELYAALRSCSE
                     KYEMPEQVLGAMNWVVCTSRYSEKYAQALSVIYSSADGEIRFANAAFNPLAYFDSERG
                     NFIDLDTKGVPIGIERGFSYESGAVKAHPDSIGVMYSNGLVNARNRDGASYTIGRVKD
                     LIRINRGDSPAVLARRILADFEQYTDGTVLTADVSLVVFKIE"
     gene            20485..21390
                     /gene="ispE"
                     /locus_tag="PKJ16_04825"
     CDS             20485..21390
                     /gene="ispE"
                     /locus_tag="PKJ16_04825"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HNV46346.1"
                     /translation="MQLTTRAHAKVNLHLEVLNKRPDGYHAIFSLMASVGFCDLLKLE
                     SVRLTERPDGECSITVHAAGGKYPELIETIPYGRNLIEMAAALYCRTAGLSGEIDFTI
                     EKNIPSGAGLGGGSTDAAAALRLLNQRLGRVSEPDLRALAAQLGADVPFCLNGGAAIC
                     EGIGDIIEPLVGRVTRHLVIANDGIHVNTAAAYASLHRGQSYPVDRDALNRKRARIIE
                     DFANGNLRNLTSIRKNDFEAPVFAEHPRIEHINAMMAAHRPEFAAMTGSGSSIIGVFE
                     SESDARSAHEALQCEVKHCILTALL"
     gene            21394..21464
                     /locus_tag="PKJ16_04830"
     tRNA            21394..21464
                     /locus_tag="PKJ16_04830"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21426..21428,aa:Gln,seq:ttg)
     gene            21497..22255
                     /locus_tag="PKJ16_04835"
     CDS             21497..22255
                     /locus_tag="PKJ16_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HNV46347.1"
                     /translation="MSAAANDITAVLLAAGKGVRMQSELPKVLHPLNGTPLIRHVIDS
                     VMAAGIRDIIVVVGYQGQQVIDALDGIPGITVAWQREQLGTGHAVLQAEQVRAGFPGP
                     VLVACGDVPLIRPAHFRSLVDAAREPNARAAVLTMFQDNPTGYGRIMKDARGFIERIV
                     EEKDATPDEKAVREVNSGTYVFDGPLLFEGLKTIGTDNAQREYYLPDVLQHIRRSGYD
                     VRAVLLEDAICGRGINSREELAALEEYLREHAPQ"
     gene            22274..23212
                     /locus_tag="PKJ16_04840"
     CDS             22274..23212
                     /locus_tag="PKJ16_04840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HNV46348.1"
                     /translation="MDHQMKIFSGTSNPVLAREIADYLNLSLSAIELKKFKDSEISVK
                     IGENVRGVDVFLVQSTCNPANDYLMELLLMIDAAFRASAKRITAVIPYFGYARQDRKV
                     EPRVPISAKVVANILHSVGVNRILTMELHSEQIQGFFDMPVDNLYAGPILVDYIKRID
                     IQDAVVVTPDTGGAERARALAKKLHAGLALIDKRRPEANVSEVMHVIGAVEGKNCILV
                     DDMIDTGGSISAAARALKENGARDIYCLATHGVLSADASMKLDAAGFKELIITNTIPV
                     PDGKRPKHTTVLSTAQLFGEAIRRIHNGESISSLFS"
     gene            23255..>23533
                     /locus_tag="PKJ16_04845"
     CDS             23255..>23533
                     /locus_tag="PKJ16_04845"
                     /inference="COORDINATES: protein motif:HMM:NF013545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNV46349.1"
                     /translation="MKAHDLTVTLRTETGKNECNRLRAAGFIPGVVYSHGGSEAVKVN
                     KKDFMGLFKGHISESVLINITVANKPADNAFKVFVKDYQLDPVTDIIKH"
BASE COUNT         4460 a         7510 c         7328 g         4235 t
ORIGIN      
        1 atgtgacgct ttgaagaaaa tgttcgccct gcgcactggc gcatcaccac cgctttttat
       61 cgatgtcctc gtagcccttc agcaccgcat gcgccgctcg gtcaaaatac agcacaacgc
      121 gattgctcaa ttccagcgcc tgtcgaaagt cggtcggatc atcgcccatc tttttttgga
      181 taaacagcca tacgtggcgc ttaagcagca tgagcgcctt gaacacatgg gacgcgggaa
      241 cgcccttctc gaagcgctcc caggcgagct tcttgtagtg ctttgcgatt tcgtctttcg
      301 aaaaatcctt ggtgatatag ctcgaaagct cccgatagat ctggttgccg gattcgtaaa
      361 tgacctgccg gtcgagcgtc cgataggcgg tcgtatcggg atcacccagc aggtcgttca
      421 tgtagtactc ggtgatctgc tgatggttct cctcgatgag ctccacgaac ttattggtga
      481 gaatattatt ttttggtatc atggcgaacc tcgctttttc aatgcgtcta cgtcccgggc
      541 cgatattaat caagaagttt ttgccgccac ccgcccgcac gaccgcggcg gcatggacgg
      601 attcgcgcat agcgtctcga ggccggccta tttcgcgaga cgccgatact ccgccagata
      661 ctccgcggcg atctcgggat tgtcgatgat gatgatgttc tcatcgttcc tgcggtcggc
      721 gtttttcgaa aaattgtacg agccggttat caccaggcgc tcgtcgatga tgagcacctt
      781 atggtgcatg gcgcgcggat tgcggtccag catcacgggg agcccctccg tgcgcatttt
      841 cgtgtattcc gacgatgcgc tcccggcccc gcgccgctcg aagatcccgt gaacaggcac
      901 cccggacttg aatttcgcga tcatcgcctc gccgatcggg tcgctcgtga acgagaacag
      961 catgaagcat atgctttttt tcgccttgtc gatccgcttc gcgatgatcc gctcgacgcc
     1021 gttctcgggc gagaaatgga cattgatgtg cgattctccg atccgcacat agtagcggtt
     1081 gcggacgacc gcgaagggcc cgggctcggc gcgattgccg aagacgcgat gggtgaacat
     1141 ctcgtcgaac tccgcctcgt agatgcgcgc gagctcgggc gagcggatca taatggcgtt
     1201 gttgttgttt ttgcgcgcgc agttccgggt gaggttgaac gacccggtcc acacgatctc
     1261 cccgtcgatg atggcgaact tgttatgcat gaacccgcgg cgttcgtcgg cgaccaccgg
     1321 tatccccgcg gaccggatcc gcgcgatctc gtcgttgccg gcgttcccgt cgtccgtgac
     1381 cagccgcacc gacaccccgc gtgcggcggc ggaaataaac gcgcgcgcga tctccggcga
     1441 ggaaatgtcg tagaaagcgc cgtggatcgt gcgctccgcg gcgccgagga gcctgatgaa
     1501 ggcggcctcc gggccgtcgc ggtcgttgcg cgggcttccc ggagcggtga aaaacacgcg
     1561 ccaccatccg gtcgcatcgc cggtccgggc atcgggcggg gcgcttccgc cggtacacaa
     1621 cgcgacgccc gccatcaccg caaaaccgta cagcaccctg cgcattcgca ttctacacac
     1681 ccgttgccac tgtttttcgc gctcattctc gaacgagccg cctgtcgtgc cgctcgacac
     1741 tccgtgatga acacgcgcgt acagattata cggacgaccc cggggaaatt ttaaacacgc
     1801 ttgggcgggc cggtgcgctc cgaggacggt cactttttca gtttttccat cgcttcccaa
     1861 atcaggttct ctatctcggt gagcctgagc tggtcgtccg acgaggggaa caggagcttc
     1921 gttttggcga tgagggtgtg atacgagacc agctcgttct ggttgacctc cgcctgtatc
     1981 atccgccgca ccgcatcgtc gtctttcatg agcaggttca gatagaaccg gaaggagttg
     2041 gacagcaatt cgagcgtggc gcgctcgcgc cggagatcgg ggtcgcggtc ggccttgccg
     2101 atcatggtga cgacgaagtt gaggatgtac tgcgcgtcga tctccgcgag atcgaagtcg
     2161 agcaggagct gcttcacctt ggccaggtcc atcctgcgca ggtgcacccg ggccaggaag
     2221 acgggattct gcgacagcat gttgatgttc acgtccttga tgagctcggt ggcgcgggtc
     2281 gtatcgatcc cctccccggg ctgcggcggg gcgtcggcgt caatcggctt gtccccggtg
     2341 atgatcacct gcacggcggt gcagagattt ttgatcacga tgatgtcgtt ccgggtgatc
     2401 gccgtgtcca cgataatata atcgggcgcg cgtccgattt tcacctcgtt ggccagcaga
     2461 ttgaagttcc gcaggcactt gacgaccacc ggcagcacgt ccagggcctc gtcgcgggcg
     2521 atgaagacgt agcgcttctc gtcctgaaat tcgtacggga tgacgagctt cccccggcta
     2581 atcttcacga acgacaggtc ctcgccggcg gtattcttca cgtgaaagcg ctcgcccagg
     2641 aaaaagaccg acggggcctt gtgcatcagg cgaaggttgt ggccatcggc gtacagggac
     2701 gatccgttcc ggtcgaaggc gagaaattcc cgcttcgcgg gatacagctt gatgaggatg
     2761 cccttcggcg ttttgtgttc gatatagtcg gtcatgatcg caacgcgccg caaatcgcga
     2821 ccggcgcgca ccggcgcaag tctatcgccg ataatttatt tgacaacaat atattttatt
     2881 gccatgaggg ggttacgatc aaagggggcc atcaatgcat tccatcaacg tgagccgtcg
     2941 cgccgttcac gcgggcgcca tcgccgattc ctcgggggag ctggacgcca aatactccgt
     3001 gaaaaacatc gtgcggaacg aggggtcgtg gcgccccgcc gctcgtccgg gcgcggccta
     3061 cgattttttc accgtcgatt ttggcgcgga ggtccccgtg aacttcgtcg agatcgcgcc
     3121 gtatccaccg gcgcgcgggg cgtttcccgc ggcatttcgc atcgaggcga gcgacgacaa
     3181 tgcgagctgg cgcgtcatcc acgcggagaa gaatttcgcg ctcgatgccg acgaagcctc
     3241 gttccttctt tcgttccccc tcgcgaccct tcgctacctc aaggtggtga tctatcattc
     3301 cggccaggaa aaaaccgacg cccctgagat cgcgcggctc tccgccggca tcgccggggt
     3361 gaagaccatc gtcgggccca gctcgtccga ggcgcatgcg ccggcgacgc tcgtgagccc
     3421 cgcgccgggg gcctactggg aatcggccat caatcagaac cgcgcaacgg ccgtcctgga
     3481 catcgacctc gggacgagct gcaatcttgc ggcgatgacg ctgaccgccc acggcggatc
     3541 accctgcgcc tttcccgagc acttcctcct cgagctctcc gcggacaaca agctctggac
     3601 gacgatccgc gaggagaaga attttgcagc cgagccgaag gcgaaatacc gctgggactt
     3661 tcccgtcacc cccgcccgct atgcgcgcat cacggcgacg gacgtgaagc tctcgcagaa
     3721 gcacttcggc gtccgccttg cgggggtcga gctctttgcc gtgctgccga acacggggca
     3781 cacgcacaat atcagcggcc tcacgctgcc cgcctccgtc ttcaatcccg gcatcgtgcg
     3841 gctcgcgcgc gacggcgaga ccgcgcaggg cgcggcggtc cagggaaacg acagccggct
     3901 ccgcgacgcc tccacgatct tcaaggggat cgtccagctg gccgaccacg gctcgacgga
     3961 ggacctgcgc gccgtgcagg cctccgatcc gcggctcgcg cccgccacgg acggcaggca
     4021 cggcaccgtg cggctcgcct atgaccgcga ggtgaacccg aacgccgtcg tgcgctccga
     4081 cgactctcga ctgtccgagg cgacggactc gagctacggc atcgtgaagc tgtgccccga
     4141 cggcgtgtat tcgaagatga cggtcgtgac gggcaacgac agccgcctca agtcggccac
     4201 gacctcgtca ttcggcatcg tccggctggc ggatgacggc gagaccgcgc aggattgcgt
     4261 gatcaccggc gccgatcggc ggcttcgcga cgcatcgaca ctttaccggg gcatcgtgga
     4321 gctcgcggaa aacggcgagg acgcgcccaa tgtcgccgtt cagggcaacg acaaacggct
     4381 caaggacgcc accacggcaa ccaagggcat cgtggagctc gcggaaaacg gcgaggacgc
     4441 acccaatgtc gccgtccagg gaaacgacaa acggctcaag gatgccacca cggcaaccaa
     4501 gggcatcgtg gagctcgctg aaaacggcga ggacgcgccc aatgtcgccg tccagggaaa
     4561 cgacaagcgg cttaaggacg catccaccac cgcaaagggc atcgtggaac tcgccgaaaa
     4621 cggcgaggac gcgcccaatg tcgccgtcca gggcaacgac aggcggctca aggacgcaac
     4681 ggaatcgcgt ccgggcatcc ttcgctttgc gaaaaacctt gaatcggccc cgctttgcgc
     4741 cgttcaggga aacgatcatc ggctcaggga cgcaaccacc gcctaccggg gcatcgtaga
     4801 gctcgctgaa aacggcgagg acgcgccagg cgtggccgtt cagggaaatg acaaacgcct
     4861 caaggacgcg agcaccaccg cgaaagggat cgtggagctc gcggaggacg gcgaggacaa
     4921 ggcgggcatt gcggtgcagg ggcatgatcg ccgcctcaag aacgccacag aaacgacgac
     4981 cggcatcgtg cggtttgccg gaaacggcga gtccgcctcc aatgcggccg tccagggaaa
     5041 tgacaagcgg ctcaaggacg ccaccaccac cgcgaagggc atcgtggagc tcgccgaaaa
     5101 cggcgaggac gcgcccaatg tcgccgtcca gggaaacgac aagcggctca aggacgcctc
     5161 caccaccgcg aagggcatcg tggaactcgc cgaaaacggc gaggacgcgc ccagcgtcgc
     5221 cgtccaggga aacgacaagc ggctcaagcc ggcctccgtc ggcgcgccgg gcatcgtgtc
     5281 cctcgcggcc cacggcgaga cgcgcgaggg gcgtgtcgta caggcgaatg atccgcgcct
     5341 gtccgacgcg cgcacgccgc tgccgcacga tcacgactac gcgcgccggc gtcacgattt
     5401 cagcgaacac cgcggcacca tcgccatcgc cgacaaaaag aacgagccgt ttatcggcct
     5461 cacgccgccg ccggagggcg ccggcatcat ctccgcaaag aacatcacca tcgacgacgg
     5521 cggcgtgggc gtggccgggg tcgcgctcca cccgggggaa tcgtacggaa agtcgtacgg
     5581 ggtcgtcgga catgcgcaat tcgtgggcat gcgcggacaa tccacgggca gcgccgaggc
     5641 gtccgcacgg gggtgcgggg tgatgggcct gtcgcgcttc ggcgcaggcg gcgtgttcgc
     5701 cagcgagcac ggctattcgc tcgtcgtgga aggcttcggc gacatcgccg actacgacga
     5761 ctccgcacgg ctggccggcg acggtcgcgc gctctatctg cgcgggagct ccgatatcaa
     5821 cgggcggatg tccatcatca acacccagag cggggcgccg gggaacatcg tggagatgta
     5881 cgaggtggac gacgtcgatt acctgtcgcc cggaaacctg cttgtcgcgg gcgccgcggg
     5941 gggcgtgctc tcgcgctcgc gcagccgcta cgatcgcgcg gtgatcggca ttgtcgcggg
     6001 gaacccgctc atcatcctca acggcaaggg agcaaaggac aaattctacc cggtggtcct
     6061 gggcgggaag gcggtgtgta cggtggacgc gcgcgagcgc cccatcaagc cgggcgacct
     6121 catcgtgagc tcggacaccc ccggctgcgg catggccgga acgatcgatt cattcgagaa
     6181 aatcggcacg gtgatcggga aggcgctcga gggactcgag agcggcatgg gatcgatcat
     6241 ggtgtacgtg gtgcacctat aggcgcttcc cgggcgctcc cgcccctagc gggtcgtcgg
     6301 cttcccggcg atgaggtccg ccgccatacg cgcatagagc tcggacagcc gtgcgggcat
     6361 ctcgcccttc gcgtgcgcta tcgtcatggg cgtgcgcacg aaactcacga tggtccggtc
     6421 ggcgacgcga tacacgccgc cccaactttc atgcgagatg gcggtcccgc ccagcgtaac
     6481 acggccccgc gaatgcacca cgagaacgtg cgtgaacatg aaatcgcgcg gcacctggcg
     6541 gcttgcgacg agtgatttcg tcatacggcg caccaccacc tcgagcgcca cggcgatccg
     6601 ctcacggccg ccgtcaagtc gccattgccc cgcgcgcatg gcggcgtcca tggcctccgg
     6661 aagctcgaag gaaagattgc cggccatatc gtagaggcga ccatcccaca ccgcgttttt
     6721 tccgaggcgt gcggcgatcc ccgaggccag cgtcggccag ctcttgcggc tgtcgatcgg
     6781 tgcgccggta tagtcgaggt tgatcggaaa tatcagcacc ggcgccccct ccgtaagcgc
     6841 gagcggaaag gtgacgcggg gatgtacgcg taccgtcgag gcgcatccga gggcgcacag
     6901 cgccacgagg cacagcaggc cccttcgtgt tttcttgttc attctgatgg actccacgta
     6961 ccgtgatttc cgcgcccggc ccgcaagcgg cgatggcgcg ttcaacattt cccgcggcgc
     7021 cgcgcgcggc aatgcgccat cagggcgtga gcccctcgag caccttccgc tcgcccgccg
     7081 tgaacacctt gagctggtaa tcgttgatct ctttggcgat gtccttcccc acataggtga
     7141 gcttgcgggt gtagggcttc ttctcgtatt catcgaccca caggatgacc gtcgtcttgt
     7201 cggcctccca gacataggcg ccctggtttt tcttgatgtt ctccccggtg gggacgccga
     7261 acttcgcctc gacctttttc cgcacatcgt ccaacgcgag atacggaaag ctgacgacca
     7321 cgtagtacaa gcgcccgtcg ctcttggcgt ccttggcgtc ttttgcctcc ggggccgcgg
     7381 cgacggccgg atcaacatag aaaaagccgt acaggtattc gatttccccg tcgcgggaga
     7441 tgatgacctt tttctcgtcg gtgtagacga ttttacccag caccccggtt ttcgacgccg
     7501 ccgtgagcgc gccccatttg aggctgccgt agccgtccgg aatgttcttt gccgtttttg
     7561 ccgatttgtc ctccgccgcc ctgcccgact gccgggtttg cgcatacagc gccgtaccga
     7621 gcgccatgca gatcaacact gcgatagtaa tctttttatt catgcccgct cctttctcta
     7681 aacggaacgc atgcgcggtc cgccctggta cgcatatcgg catattcgcg aaaaaaaaca
     7741 agcgcgaatg acggctccgg caccagacgt ttattgtttg acagaatccc cgcgcccgat
     7801 accaccgtat cccgtgccgg agcgtcctcc cgagcgcacg cacaccgggg aaccatgtcg
     7861 aaacaaaaaa ttgccgcatt ttccgtcgcg cgatatgctt gtatcgcgct catcgctgtc
     7921 ggatgctcgg ggctctcgag gctggaccac acggaccgcc tggggagggt cgcgagcaga
     7981 gcggtctata caaatggcgt tctgcgcggc gcggaggaga tccggtacgt tggcgaatcc
     8041 gcccggccgc tttcgatcac gtttcggtgt ttggaacgag acgcactgaa acaatcgcga
     8101 gaggcgtacc atgaatacac cgaagccggc atgctggtac ggatacggta taccatagtc
     8161 gttgacgggg aaaagatccc ctgcgggtcc gcccagtacg cgtacggcgc ggggaatacg
     8221 ctccggcgga tcacctacac cgccctctcc gataccgtcc ggagacggac gtatccgtcg
     8281 ggtgtgcaga tgttcgaata ttccggagga cgtgtcgcgg cgcggcgcat catcgaatac
     8341 gcctacaacc ggaagataaa cgccgtgatg cagattgggc agcacgcgat ttcctatcat
     8401 aacgccgctc cggccctgct gcaatcatgg gtgctcgatc ccgagaccgg cgttatcgcg
     8461 cgcacccgcg aaacgaacgg gacggcgctg cgggagcgca tcacacgcat cgaaaccgcc
     8521 cttgccgctg cggcgagcgg cgtacatttt acctgcacca attaggagga gctatggctg
     8581 gactcattga agcgacagac gcgaatttca agaccgaggt gctcgacgcc tccggcacgg
     8641 tccttgttga tttttgggcg ccctggtgcg gcccctgccg gatgcagacc ccgatcctcg
     8701 agaagctcgc cggctcgggc gagatcggcg cgaagatcgt aaagctcaac accgacgaca
     8761 atccgaacac cgcgcaaacc tacggcatcc aatcgatccc gacgctcatc atctttaaaa
     8821 acggcgccga gaccgaacgc atggggggcg tccagcccga gaaggcgctg aaagacaagc
     8881 tgaagtagcg ccggggccat gcgcgcggtc ctgcagcggg tgctttccgc cagcgtgact
     8941 atcaacgggg aggtggtctc ggccattggc ccggggatgc ttgcgctggt cgggttccgt
     9001 cgcggcgaca cggacgacga catggcctac ctgatcgaca aaacgcttgg cgtccgcatc
     9061 ttcgacgacg agcgcggcgt aatgaaccgc tcgcttgcgg agatcgacgg cgaacttctg
     9121 gtagtgtcgc agttcaccct ttacggcgac gcgcgtaagg gacgccgtcc gtcgtacggc
     9181 gaggcgatgc cgccggatga ggcgcgcgtg ctctacgacg cgtttcttcg gcggtgccgc
     9241 gagcgccacc cgcgggtgtg cggcggtgtg ttcggcgccg acatgaaggt tgcgctcgtc
     9301 aacgacggcc cggcgaccat cctgctggac agctcgcgaa acctgtaatc ggccgcaccc
     9361 atacgcggta ttcacgaccg cgttttttcg tcacaacaat cgaaagcgga atcgtatctg
     9421 agatagcact catgcgcgga gatgcgatat gccgattgat acgacgctgt acatccatga
     9481 aaacacgctg aaactgatct ccgagtcgtg cgacatgagc ggtatttcgc acaaccggct
     9541 catcgtcatg ctgcttaaga atgcgatgag taaagcccgg aagaacatga aaaccggttt
     9601 ggccattcgc taccaggagc ggaatcaggg cgggcactgg cacacggttc atgtgcgctt
     9661 tcgggaggac gaggcggatt tctttaagga tatgcggaac tttatgaaga tgtcggtttc
     9721 gctgattttg gctcaggccg cacgagagta tcttaaagaa atggtcagag catcagcgga
     9781 aagggaaacg accgataact atccgttttc gaaccatcaa ttgtcattta ttgaaaccaa
     9841 tgacgggatt ttctggataa cacactggaa aacaccccca cccccgtcac ccacaatcaa
     9901 acattgaacc ggaagctgat gatgtccccg tcctcgacga tatactccct gcccatgaca
     9961 tggaaacgtc ccgccttctt cacggcatcc tcgctcccgg cctcgatgag atcgggatac
    10021 ttcatcacct cggcgcggat aaacccgcgc tggatatcgg agtggatcgc ccccgccgcc
    10081 tcgggcgccg cggccccccg acgcacctcc cactgacgca cctcgtccgt acccaccgtg
    10141 aaaaacgaga tgaggccgag cgcatccata cacaggcccg tgagcacgtt gagcgcgggc
    10201 gcggcgatcc ccgaggccgc catgaactcg cgccgttcgg cctcatcggc caggagggca
    10261 atctccgcct cgagcttcgc gcttatgcgc atgcacgcga tccccgactc cgtgagcgcc
    10321 tcctcgaccg cagccgaaat acagccggag gcgatgtccg catcacccgc attcacggcc
    10381 acaatcatct ttttcatggt gaggaacgga tagctcgcga tgagcgccgc ctcctcgtcg
    10441 cttatcgcgc gcgtccgaag cggccgccct gcgtccagat gctctcgaaa cgacgcgagc
    10501 agcgcctcct cgcgcgccga acgctcgtcg cgtcccttct tctggttttt tgcgatgcgc
    10561 tccaggcgct tttcaatgaa cagctgatca tgcagcacaa gctcgtcgtt taccagcgcg
    10621 atgtcgcgcg ccgggttcac cgaaccggag acatgataca cggcgtcgtc ctcgaacgcg
    10681 cgtacgacat ggcagagcgc gtcggtctcg gccgcgatcg cgaggacctc gggacggacg
    10741 ctcccggggt cgatccgcgg aacaaggtcc acggcgatgc gcgcgcgggt ttcttttttc
    10801 ggacgataga gggccgccag ccggtcgaaa cgctcatcgc gcacctcggc gatgccgctg
    10861 agtgccttca tctggtcctg cggcgcgctt ggcgctttgg cgccggtgag gagttcgaaa
    10921 aagcttttct ttccggtctg cggcagaccg atgatcccga tcttcatgca ttcattctcc
    10981 gtgcgcgggt gcgcgaacga acctgcatag cgcgggggcg gaatcgatca agaacaattt
    11041 ttcgggcgca gcggcgtcgg gcaatcaggc gggggcggga ccgtcgatca cctcgcgaat
    11101 ttttttggcg aggtcctggg gacgataggg cttagcgata aagttaagcc cgcggtcaat
    11161 gatgccgtga tgggcgatca cctcctcgga gtagccggat gagaaaagaa tccgaagccc
    11221 cgggcgcagt ttcccgacgc gctgcgcgag ctcgaggccg ttcatccctg gcatcaccac
    11281 gtcggtgacc atgagatcga tcgcccccgc gtgggcctcc acggcgtgca gcgcctccat
    11341 cggatttccg aagaccatga gccgatagcc cagccgcgag agcagacgca cggcgaggcc
    11401 gcgcaccgcg ggatcatcct ccacgagaag gagcgtttcc gtcccgcgcg ggaaatcgcc
    11461 caccaacctg cccggtagcg ttgcctcgga gccatcggcc acacgcggga agaagatctt
    11521 gaacgtgctc cccaggccaa cttcagaata cacctctatt gagccgctgt tttgcttcac
    11581 ggccccatag atggtcgcca gccccagacc ggtccccttg cccttgggtt tggtggtgta
    11641 gaacggctca tagaggtgct ccttcacctc cgaggacatg cccgatccgg tgtcgctcac
    11701 cgagatgagc acgtagggcc ccggcgacac atagggatgg ccggacgcgt attcctcgtc
    11761 gagatcgagc gtcgcggtct cgatgatgag ctcgccgccg ccgggcatgg cgtcgcgcgc
    11821 gttcacggtg aggttgatga tgatctgctc gaactgtccc gggtcgatcc gcaccgcgcc
    11881 cgcgtcggga gcgaggaccg tcttgagcgc gatgtcctcg ccgatgatgc gcccgagcat
    11941 cttcttcatg ttggaaacca ggtcgttcac gcccactacg cgcggctcca cgatctgctt
    12001 gcggctgaag gcgaggagct gccgggtgag cgccgccgcg ctctcggcgg ccttcatggc
    12061 gtccttcagc gtctcggcga gcgcgtcgcc ggcgggcacg tccatgagcg ccagcgagat
    12121 gttcccggtg atcgccgtga ggagattgtt gaagtcgtgg gccacgccgc cggcgagcct
    12181 cccgatcgac tcgatcttct gcgactgccg gagctggtcc tcgagacggg cgcggtcctc
    12241 ctcggccttt ttgcgctcgg tgatatcgct cagatagccg tcatagtgcg tgacggcgcc
    12301 ggccgcgtcg cggatcacca cggtgaagtc gtgcacccag cggacctcgc ccgaggcggt
    12361 aaaaacacgg tactcctgtt cgtacgacgg cgtgcccgcc tcgccgtagc gcgccacctc
    12421 gcgctcgatc cgctcacgat cgtccggatg gatgatatcg aggtagctgg tgcgcccctc
    12481 gatgaacgac gccggatcat atccccagcg cgacacgttg ggcgacacgt actcctccgg
    12541 ccagccgggc tccgcgcgcc acttgaaggc gaccatgggc cccgagacga actgattgcg
    12601 ttctcgctcg agcgcctcct cggccttctt gcgctcggtg atgtcgttca tggccgtgag
    12661 caggtagcgc tcgccctcga gttcgacgat gtccgcggaa aacgagccgt tgcgcaggac
    12721 ccccgagcgc gtgcgcacct gcacgtcgaa atcgcgcgca aacccgtcgc gttccagcgc
    12781 ggacatgatc tcgtcgcgac gcgagacgtc gtcgaacagc ccgagatcgc gggaggtttt
    12841 cccgatcacc tcctcgcgtg aaaacccgag cacgtccagg aagctctgat tgacctcgaa
    12901 aaactggccg gtgtcgatcg tgctgatggc catgagcacc ggcgtactat tgaaggccgt
    12961 gataaacttc tgttcgctcg cgcgtaacgc cgtttccgcc ttcttgcgct cggtgatgtc
    13021 gatattgaac tccgccacca gccactcgcc gtcggggttc tgaaacggga tggtgtgcac
    13081 ctcgatggcg cggttcccct cgcggggcag cacctcctcg gtgatcgtga tctgccggct
    13141 ggtgatcgcc gccgggatcc cgcagtccga gcacggcctc tgacgaccca tgaaatactc
    13201 gtagcatttg cgcccggccg gatcgccgta aaccttctgc cagccgggat ccacataccg
    13261 gagattgtaa tcggcgtcga tgatgtccac cccggtccgg gtgatgctca ggatgctctc
    13321 gagccgtttc gtggtctcgg cgagctcgcg cgcggcgcgc ttgcgctcgg aaatatcctt
    13381 catggtcccc accatccggt acgggacccc gttccggtcg gggatgaagg cgcctacgtc
    13441 ctctatgtcc atgtacgagc cgcccttcac gcggaagcgg tattcgatac tgtagagcgt
    13501 tccggtgttg atggagcgct ccagcgccgt cgtggcgcgc gcccggtcat cgggatggat
    13561 catctcctcc cattcgttga tgtccaccgc gcgcatctcc tcgggcgtga agccggtgat
    13621 ggcggcgatg gcgccggccc agttgatgac gcccgcggga atatcgtaat cgtagacgag
    13681 ctggcccgta gcctgggcgg cgagccggaa gcgctcctcg ctctcccgca gcgcccgcgc
    13741 cgcccccacg cgctcggaga tgtcgcggca gagcgtgagg atgcgcgccg cgtcgccgct
    13801 cccggtgacc gtcgcattga tctcgatggg tatgatgact ccgtttggcc attcgtactc
    13861 cacctcgaag ccgcggataa agccaagtgc cgcgcactgc gccaccgccg cgtcgttgcg
    13921 ctcccggtcg tgcgcggccg tccactcgag gacgtttcgc ccgcgaatct gctccagcgt
    13981 cgcgtggccg gtcatcttca cgtagatgtc gttcgcgtcc acgacatgcc cgcgcccgtc
    14041 gacaataacg aagccggtat cggtcgtttc gatgagccgg cgatacttct cttcattgtc
    14101 gcgcaatgcc cgctcgatgt ccttgcgcgc cgtgatgtcg gtgaaaaacc cgacattgca
    14161 ctcgcggccg tctatcatca tcgccgaggt gttgatgtcg gcgtagatga ccgtcccgtc
    14221 ttttcggagg caggggatgt ccgccgcaag aatcgtatcg ccgcgcgcct ggctcataaa
    14281 ctcccgcact acggatgcga ccgcctcggg gggatggata tcgcccacgg acaggcgcag
    14341 cagctcctcc tcggtgtagc ccagcatttt acagatcgcc gggttcgcgt aggtgaaccg
    14401 cttcgattcc acctccgcga ccaggatccc ctcgcccgcg aagtggaaca aggtccggaa
    14461 gcgcgcctcg ctctcgcgca gtcgccgctc catgcggtct ttgtagagcg ccgattcgat
    14521 gttcgaatgc aggtccgtcg agctcacggg cttggtgaca tagccggagg gcccggtgtc
    14581 gcgcgcggcg tcgatcgtcg cggggtcggc gttggcggtc atgtagatga tggggatggg
    14641 cgcgttcgaa cggatggcgc gcgcggcgtc gatgccgctt gccgcgccct gcagcacgat
    14701 gtccatgatg atgaggtccg gcgcgagcgc ggcggccagc gacaccgcct gctcgccggt
    14761 cgtggcggtc ccggccacct cgtagccgag gtcggtaagg atatgcgtca cgtcgagcag
    14821 aacgatcggc tcgtcctcga cgaggaggat tcgggggcgc gggggattgc tgtcgcttac
    14881 atttatcgcc atggcattcg tattctcaac gatgggaaca tatcaatccc aactgccctg
    14941 aagcggctta atgctggcac atcatggcaa ccgagtcaac acaatttacc gccccgaggt
    15001 gaccgatggc ccatcgcgaa acaccagtga ccgacacgct gcccgtgcaa cgctgccggg
    15061 acggcgccga cattcgcgag ctcgccgacc acgagctcgt cgcaatcatc atcggaaccg
    15121 gaactcgcgg gaccggggtg gtgcggatgg ggatggaaat cctggagcga ttcggcgggg
    15181 tgcgcggcat acggtgcgcc ggcctccgcg aactcgcgaa tgcgaagggc atggggatgg
    15241 tcaaggcggc gcgcctgcag gcggcgctcg agctgggacg gcgggccatg gcgacggtcg
    15301 aaccgggcgc gtcggtctcg tcgccccggc gcgtatggga gctgctccgc cacgagatcg
    15361 cgggcgtccc gcaggaggtg tttcgcgtgc tcgtcctcaa cagtaaaaac ctgctggtga
    15421 agaaccgcgt ggtctcggtg ggcaccatct ccgccgcgct catccacccg cgcgagattt
    15481 accgcgacgc catacgcgaa acgggctcgt ccatcatcat cgcccacaac cacccctcgg
    15541 gggtccttac cccgtcgggc gaggacatcg ccgcgaccaa gcgcatcgcc gatgcgggga
    15601 acatcatcgg cattgcgctt ctggatcacg tgattattgc cgaggcgggg tacctgagcc
    15661 tgcgggaggc ggggtatatg tgaacacgtt catgacaaag ttggtatgat tgcagatcca
    15721 ggcctcgtac acgccgccgc cctgccacac ggtgatcccc accggccgcc ccggtgactc
    15781 ccgcgtggcc agcagcgcaa gcgtatcggc gcggaacgcc tgcacggtat tgtcattgta
    15841 gcaggtcacg aatatcaccg tgccgtcggg cgaggccgcg atggtgcgcg ggctggacat
    15901 cgtccgcgcc cattgcacga tgcgcccgga ggggagctcg accttgatga gcttcgcctc
    15961 gttgcagatg gacgcgtaga ggtatccgtt cgatatcagg atatgccgcg gcgcgggccc
    16021 cacggtgatc tcccgcacct tttccacggt gtccagatcg accacgctca ggaaattccc
    16081 gcccatctgc gcgatgtaga gaaatttcga atcggcgctg gcggtcatcc cccgcggcac
    16141 gctcccggtc cgcagggtct tcacgacgtt ccatccgtcc ggcgtgggcg cggaaatatc
    16201 gatcacgctc accgtgctcg aatgccagtt ggacacgaag aggtacttcc cgttcgggct
    16261 cgaccagatc accttcggcg tcgcgccggt ctttatgagc aggagtttca tcttcttgtt
    16321 cgtgaattcc ggctcgggtg cggccttctt tttcgcggcc ggcggcgccg acacgtccgc
    16381 gggctttggg ggaatgcgct cgtgcagcca tgccgtcttg aagggcttgc cctcgacggc
    16441 cgtgtcgccg cccaagaggt cccacaccgc aacgccgtcg gcgttgtgca gcgaaaccca
    16501 gagatagcgc ccgttgtgcg agaaatggcc ctcgaccggc ttttcctgat acgagttgat
    16561 cgggaccttc ttgtcgtaat cccagccctt cccgggcgtc atcacgaaga tgagcttgcg
    16621 ctggatcacg cggctggcgc ggttatactc gtacacgctg cactcctcga ggttcatgga
    16681 gtacacccgc gacccgtcgg ggctcatctc caccgacttc gcgccgattc ccgttcgaag
    16741 cgactcggcc ttcgtgatgg cgagtttctc cgggtacacc gggacgacga ccgccggctc
    16801 cttttttacg gcgttgcacg atagcgcaag cgcacccgcg aggaacatcg cggcgcgcgc
    16861 gcgcggaaca attctcggta atagcatatc gcatctcctt gtgttgatgt gtgcagtgat
    16921 cccgtccctc gccccgccac ggaggggggg tgtccgaaga gcaaacgggg ccgcacatgg
    16981 ctctccggca ctaacccggc gcgggcggaa aaaaatcaac aataatcccc cgctacgaac
    17041 cgccccaaag atttcccagg cggcgccgga tccgcttata cacgcccttg agctcgatga
    17101 gccccacggc gtcgcgctgc tgcgtgagcg cctccagcgt ggtcgcgcgc accacctccc
    17161 agagccgcgc gcggtccggg caggaaaaaa aataatcgag gttcacctga taggcgccct
    17221 tgaaggcgcg cccgcctccc tccggcgtga tgcgcctgat gagctcggtg agatcgaggc
    17281 tcccgtcctt cgcgcggatc gaggcgttca gggccaagcc gcgggtcgcc tcgcgcacca
    17341 ccacggcgaa gacgaccacc acggcgatct cggggtcgcg cgccatcgaa tccgcaatga
    17401 tggcgatccc gtcccggcgg ctctcgtcga tgaagccgat gcccgccagc gcccacccct
    17461 tgtacacgac gcgctctcgc tcggccaggg ccatcaaccg caccacctcc tcggagagcg
    17521 ggatgcgcgc gatgtgccgg atctcctcgg gatcggcgtg gcgcgcaaga tacgcgagcg
    17581 cccggcggtc caggtcgttc gcatgcgcgt agttgtcggt gtcggtttgg atgccgtagg
    17641 tgagcgcggt ggccacgcga cgcatgagcg gcgcatcgaa cggcggcgcg agcgcctcca
    17701 gcatgagcgc cacgagcgtg ctcaccgagc ccgccgcgcc gctgaccagc ttgaaatcca
    17761 ccggaaggtc ctccgccacc gcatcgtggt ggtcgatgtg gatcgcgcag cgcaggcgct
    17821 ccgagacccc cttgatgtgc gccgactgga aatccatgac cgcataggcg tcgtaggacg
    17881 acaggcgggg gagctcgcgg ctgaaggcga tgggaatgcg aaggtcgcgg atcatcgcct
    17941 cgttctgcgg cagcgagacc acggtgatgg acacgatggt cgcgcgcacc ccctcggcgg
    18001 cgcacagcgc gtgcaccgcg aacgacgagg cgatcacgtc cgggtcgggc gagcccttga
    18061 tgtagatgag caggtgccgg cagcgccgaa cggcgtcccg gagcgcgccg gcgtgcgcgg
    18121 cgatatcggc cggtttcatt ctattttgaa gaccaccaga ctcacgtcgg cggtaaggac
    18181 cgtcccgtcg gtgtactgct cgaagtcggc gaggatgcgg cgcgcgagca ccgccggcga
    18241 gtcgccccga ttgatgcgga tgaggtcctt cacgcggccg atcgtatagg acgcgccgtc
    18301 gcggttgcgc gcattgacca ggccgttgga atacatgacg ccgatgctgt ccgggtgcgc
    18361 cttcacggcg ccggactcgt agctgaagcc ccgctcgatg ccgatgggca cgcccttggt
    18421 gtccagatcg atgaagtttc cgcgctcgct gtcgaaatag gcgagcgggt tgaacgcggc
    18481 gttggcgaag cgtatctcgc cgtcggccga cgaatagatg accgacagcg cctgcgcgta
    18541 tttttcggaa tagcgcgagg tgcacaccac ccagttcatg gcccccagca cctgttccgg
    18601 catctcgtac ttctccgagc acgaacgcag cgccgcgtag agctcgagtc ccaacagtcc
    18661 cgactccacg ccgagatcgg cggtgtcggc gatgaatatg cccagcgtgt cagggttgat
    18721 ggcgacggcg tcgaagtaat cgcccccccg ctccgagacg ttcttgtaca gcgagcttag
    18781 ccggatgccg tcgatctgcg cgagggtgcg cgggatgatc cggcccttga tggtgcggac
    18841 cacgagccgg tcgtggctgg ccgcctgcgt ccgcttcacc tcgtcgagca tgatggcgtt
    18901 ggccagtgtg agctcgaact ggatccggta gagctccagc gcctggagca ggttgatatt
    18961 gtacggacgc ccgctcacca ggcttccggt gaggatgacg ccgatgagcc gcttttcgta
    19021 gtcgaacagc ggcacggcga cctgaatgtc gtgccgatcg aagagcgcga tgagctcctc
    19081 gcgcttttcg gcgagccgct cgtcggtgaa gatcatcgag cggtcgatga gcccggcgta
    19141 ctcgacgagg gcgcgcgcga gctcgccgtc cgcgggcagg ggcgagagcg atatccgctc
    19201 gcccaggctg tagctgaatt cgaaggcgtc gcccgcccgg tcgcgcgtga gaaaggtcgc
    19261 gcgcgcgatc ccgaagcggt gctccagcat gtcgatggcg ttgttgaaga actcgacgta
    19321 gccgcgctcc cccgcgcccg cgagccccga aagctcttga aagaactcga gcacgttgcg
    19381 ctcgaaggcg cggtactcgc tctggaaaaa ccgttcgagg cgcggacgcg cgctgcgata
    19441 gaacacgaac aggtatagga acacgccgaa ggcgagcgcc ggcgccggga ccgtctggcc
    19501 aaacacgacg cgccccatcc cgaattcgac ccccgcatag accggcccca ccagcagcgc
    19561 gaacaccgtg acccagtata tcccgcgcag gtagaagcgc ttgaagtcca gcatccgctc
    19621 gtcggagagc gcgtagttga tgaccagcag gagcaggcta ccggacaccg caatggcgat
    19681 gttcttgtat gcgtgaacat tgaacatgat gggcagcata aaggtgaaca ccagaatcag
    19741 cagcgacccc aggttggtcc ccatgaacat gtagaagatc tgcacccgga acgactggtt
    19801 gcgaagaaag ctcgacttgt acaggaggat gagaaacgag ccgaaaatat agagcccgaa
    19861 gagcgcgata taggcgataa agaagcgccc gaaccggaag gccaggtggt cctgctgcac
    19921 cgtctcgacg acgatggcgt ccgtgaaaag gagcgcgccc gcgaggacca ggccgggcag
    19981 ggccgagagc gcgatgaacc atcccggcga gcgcttctcc cagcggggaa agatctccgt
    20041 caggtgaaag aagagctggg tggccccaag gataagtaga aaaaacagcc ggcccagctc
    20101 cgtgcgatac tcgaagcccg gcgccacgag caatgccccc aacgaaaggt acaggcacag
    20161 cgtgatgaag ctgatgaggg cgaaaacccg gttgatgacg aaccgcgagc ggcggacgat
    20221 attcacgagg gcgaagtacc ccgccagcac ggcggcgcag cccgtgagga cgatttccag
    20281 agtgaggagc ggcgataatg tcatgggcat gatatggaat cggccccatg atgccgggaa
    20341 agcgctaaat ttcaagttaa aaaaaccgga cccgaccggc aacaaatatt ttcttgacag
    20401 ccaggggggc ccatactagt tttggctggc atttttcccg actggcttcg tgatcggttt
    20461 cgccagctcc acgagcggta atcattgcag ttaacgacca gagcacacgc aaaagtcaac
    20521 cttcaccttg aggtattgaa caagcggccg gacgggtatc atgccatttt ttccctgatg
    20581 gcgagcgttg ggttttgtga tcttttaaaa ctcgaatcag tgcggctcac cgaacgtccc
    20641 gacggcgaat gttccattac cgtgcacgct gcgggcggga agtacccgga gctcatcgag
    20701 accataccct atggccggaa tctcattgaa atggcggctg cgctgtactg tcgcacggcg
    20761 ggcctttccg gcgagatcga ctttaccatc gagaagaaca ttccatccgg agcaggcctt
    20821 ggcggcggga gcaccgatgc ggcagcggcc ctcaggctgc tcaatcagcg cttggggcgc
    20881 gtgagcgaac cggatctgcg agcactagcc gcgcaattgg gggccgatgt ccccttctgc
    20941 ctgaatggcg gcgccgcaat ctgcgaggga atcggcgata tcatcgaacc gctcgtcggc
    21001 cgcgtgacgc ggcacctcgt tattgccaat gacggaatcc atgtgaatac cgccgcggcg
    21061 tacgcctcgc tacaccgcgg ccaatcatac ccggtcgacc gggacgcact gaatcggaaa
    21121 cgagcgcgca ttatcgagga ttttgcgaac ggcaatctcc gcaatcttac atcaatacgg
    21181 aaaaacgatt tcgaggcgcc ggtttttgca gagcatccgc gcatcgagca catcaacgcc
    21241 atgatggcgg cgcaccggcc ggagtttgcc gccatgaccg gatcgggatc gagcatcatt
    21301 ggcgtattcg agagtgaatc cgacgcccgg agcgcgcatg aggcgctcca gtgcgaggtg
    21361 aaacactgca tattgacggc tttattgtaa cagtgaggcg tagccaagcg gtaaggcacc
    21421 gggttttggt cccggcatcc aaggttcgaa tccttgcgcc tcagatattt tccagcagaa
    21481 gagcgaaggt atccccatga gcgccgcggc gaatgacata acggcagtac tcctggcggc
    21541 cggcaagggc gtgcggatgc aatccgaact ccccaaggtc ctccaccccc tgaacggcac
    21601 accgctaatc aggcacgtca ttgacagcgt tatggctgcc ggcatccgcg acatcatcgt
    21661 ggtggtgggc tatcagggcc agcaggtcat cgacgcgctc gacggaatac ccggcatcac
    21721 cgtagcctgg cagcgcgaac agctcgggac gggacatgcc gtgcttcagg cggagcaggt
    21781 tcgcgcgggc tttcccggcc cggtccttgt cgcctgcggc gatgtgccgc tcattcgtcc
    21841 ggcccatttc cggtcgctcg tcgatgcggc gcgggagccg aacgcccgtg cggccgtcct
    21901 caccatgttc caggacaatc cgaccggcta cggcaggatc atgaaggacg ctcgcggttt
    21961 tatcgagcgc atcgtggagg agaaggacgc cactcccgat gaaaaggccg tccgcgaggt
    22021 caactcgggc acctacgtgt tcgacggccc cctgcttttc gagggcctca agactatcgg
    22081 caccgacaac gcccagcgcg aatactacct gcccgacgtg ctacagcaca tccggcggtc
    22141 cggatacgac gtacgcgccg tcctcctgga agacgcgatc tgcgggcgcg gcatcaactc
    22201 ccgcgaggag ctggccgccc tcgaggaata cctgcgcgaa catgcgccac agtaaacgga
    22261 gacggctata ccgatggatc atcaaatgaa gatattttcg gggacctcga acccggtcct
    22321 cgcccgtgag attgcggact atctgaacct gtcgctgtcg gccatcgagc tcaagaaatt
    22381 caaggacagc gagatctcgg tcaagatcgg cgaaaacgtc cgcggcgtgg acgtgtttct
    22441 cgtccaatcg acctgcaatc cggcgaacga ctacctcatg gagctcttgc tcatgatcga
    22501 cgcggccttc cgggcctcgg ccaagcggat cacggcggtg atcccctatt tcggctatgc
    22561 gcgccaggac cgcaaggtgg agccgcgcgt tcccatatcg gccaaggtgg tcgcgaacat
    22621 cctgcactcc gtcggcgtga accgcatcct caccatggag cttcactcgg agcagatcca
    22681 ggggttcttc gacatgccgg tggacaatct ctacgccggc cccatcctgg tcgattatat
    22741 caagcgcatc gacatacagg acgcggtggt ggttacgccg gacaccggcg gggccgagcg
    22801 cgcgcgggcc ctggccaaga agctgcacgc cggactcgcg ctcatcgaca agcgccgtcc
    22861 ggaggcgaac gtctccgagg tgatgcatgt catcggcgcc gtggagggca agaactgcat
    22921 cctggtggac gacatgatcg acaccggcgg ctcaatcagc gcggcggcgc gggcgctcaa
    22981 ggagaacggc gcgcgcgata tctactgcct ggcgacgcac ggcgtcctct ccgcggacgc
    23041 gtcgatgaag ctcgacgctg cgggtttcaa ggagctcatc atcaccaaca ccatccccgt
    23101 gcccgacggc aaaaggccca aacacacgac ggtcctctcg accgcgcagc ttttcgggga
    23161 ggccatacgg agaattcaca acggcgagtc catcagctcg ctcttttcgt aaaacccgaa
    23221 cagggaaatc aatccaatac aaacggagac cataatgaaa gcacacgacc tcaccgtcac
    23281 actgcggacc gaaaccggaa aaaacgaatg caaccgcctg cgcgccgccg gctttatccc
    23341 cggcgtggtg tactcgcacg gaggctcgga agccgtcaag gtgaacaaga aggacttcat
    23401 ggggctcttc aaggggcaca tctcggaaag cgtgctcatc aacatcacgg tggccaacaa
    23461 gcccgcggac aacgccttca aggtgttcgt gaaggactat cagctcgatc cggtcaccga
    23521 catcatcaag cat
//
LOCUS       DAORHF010000034        23320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1430_length_23320_cov_6.073845, whole genome shotgun sequence.
ACCESSION   DAORHF010000034 DAORHF010000000
VERSION     DAORHF010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 23320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23320
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1430_length_23320_cov_6.073845"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(470..1315)
                     /locus_tag="PKJ16_04850"
     CDS             complement(470..1315)
                     /locus_tag="PKJ16_04850"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV46350.1"
                     /translation="MKKLNVFDPNDLKEFEPDAKVGLIATVSEGELPHITLITALQAR
                     STTELIWGQFSEGMSKRNVRKNPRVGFLIMSLSKELWRGKAVWKKALNEGDEYIMFNK
                     KPMWRYNSYFGLHTVHYMDLVETTGREKLPMGGIVRGALMTKCAKAGASSGEQARILK
                     PFAEDLFNDIGALKFISYIGSDGYPVIIPAIQCQAADSRRLAFAPTAYREELAAIPAG
                     TTVAVFGLSLTMEDVLVRGTFRGFSRHRGVKLGTVDIDWVYNSMPPKQGQIYPEIEMR
                     PVVNF"
     gene            complement(1451..2482)
                     /locus_tag="PKJ16_04855"
     CDS             complement(1451..2482)
                     /locus_tag="PKJ16_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNV46351.1"
                     /translation="MKSERDAIKGLVLNIQRMSTEDGPGIRTTVFVKGCSLACAWCHN
                     PESIPFERQIQWIETKCIGCRTCVKGCPQQALSDDAGVIALDRAACTLCGICVDECPA
                     LAMELLGTEWGVDELADEVEKDRSYFESSGGGVTISGGEPCMQPEFTAAFLRECTRRG
                     LHTALDTCGLCRKEAFDAILPHADLVLYDIKEIDPVLHKRFTGSGNERMLENLKHVRD
                     TIAKTGSKTKLWIRTPIIPNATARDENIAGIGAYIAENIAGAVERWELCAFNNLCRDK
                     YRRLDLEWDFGAEALLGRETMEHLANVARHSGVDPVIVHWSGATKFISDDEQADNDAK
                     AHMRLVKGC"
     gene            complement(2591..5104)
                     /locus_tag="PKJ16_04860"
     CDS             complement(2591..5104)
                     /locus_tag="PKJ16_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV46352.1"
                     /translation="MCAASTAPAFKIKEPKNLSPRIQWLRDYYFVGARRAWNNEYSAW
                     TTGTDWDVQFDEISYYIVPEMYAFNQTFRSSFRQSARVVTLHPDFWSWSLPERKAWFT
                     KEVMVNCVPQELLPGDLIAGARFNLQASRCWSEKEAKARDALIYGTKGARAVMKWFHD
                     HGYGNSGATSGHLVPGHERALTIGWKGIHADLEQKYKALSESDKKGRKGAQLRAMMTA
                     ATMPRDLAAKYAALCRKLSAKEKNAGRKSELEQMAKNLDRVPWEPAETLWEAVQALWI
                     NHMLIMSDENYPGPGVSFGRFDQYLLPYWKKSMADGMDRDFAKEILKCFWIHSNTAYD
                     AMVQVGNQGITSGFGQLITLSGLGANGADMTNELTYAVLEVIDEMTPILEPKPNVRMH
                     RNTPDELLDRLIDMVSSSQGAPFLLNFDERSMAGMMREAKRANVAHLINENNVHEYAP
                     VGCLENTMVGNDRSGTVDNNLNLLKAVELALTGGKDLLEFVDPITGKRDPIRQDGPKT
                     GDATKFSTWDEFWNAYVIHTKNIIRRCVEVYEASERIRAEFCPTPYLSCLVGGCAEKA
                     LDITQGGAELSFVTIEGVTFGTTVDSLLAIKYLVFDKKECTMAELIQALRDNWEGHDI
                     LQAKAKNRAPKYGRDDNEADAMALAVMDLWAEETWQYKTKSTNRQFRPGMLSWNYWVG
                     DGYVMAASPDGRPKGQFLSNAICPSNGADINGPTANANSVGKVLGGTADGGAADWEGY
                     LSNLPNGASHTITFNPSILRDPEHRKKFKAFLRGYAKNGGTALQINMIDADMLRDAQQ
                     HPQNYRHLLVRVTGYNAYFTAIGRELQDEIIARESHNRY"
     gene            complement(5170..5901)
                     /locus_tag="PKJ16_04865"
     CDS             complement(5170..5901)
                     /locus_tag="PKJ16_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNV46353.1"
                     /translation="MSDRLSETIFTKLQKAIIVGDMPAGSRFPAERDLAEEFGVSRST
                     VREAVGRLAKSGLVETRPQSGTYVSDYQAKASLDVLIDIMQNGGEVDSDTLRALMETR
                     LLIELHAVKMAVAAFTPDDERELRDIIGIEERSLSDPAAMAECDYRLHALIMKRSGNI
                     IIQLVFNSFRPVYRFYTDFFFNLPEAIRITMEQHRRFVDAACRKDAHQAERIMREALE
                     YGQARVVDALGIGIENRKIRLVSNI"
     gene            6241..7338
                     /locus_tag="PKJ16_04870"
     CDS             6241..7338
                     /locus_tag="PKJ16_04870"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNV46354.1"
                     /translation="MGTFSYNPEAIANLKITDTSILDRLVDFESARAAHLAKDHSRRD
                     KRMSLSEAISAFVADGDIATDAGFAYVRTAHQAYFEIMRQGKKGLHFIGSPNTNQSYM
                     INFGVCAYSHNSYTGAEMRGTDRNYSRQLKNGKVKILSEWSHGSMAQGFKAAQLGVPG
                     VFSKQLLGSDILRYNPYVRVMQNPMRDDPDPVVFIPALYPDVTVIHVHAADKTGNARI
                     YGPTVNDIALAAAARKVIITAEEIVPEIDIRYNSKGVAIPFMYVDAVVEMPYGAVPGN
                     MPGHYYWSRQWWEKAFRYACVSDDNMKAFMDEWVFGCKDQFEFVDKLGGAKWITMARR
                     LTKAAEYDNESDGVDFSYQPYLPDKDPGIYY"
     gene            7352..8140
                     /locus_tag="PKJ16_04875"
     CDS             7352..8140
                     /locus_tag="PKJ16_04875"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HNV46355.1"
                     /translation="MTTNKPANPLEMVAYVLSRQISDHQVVYVGTGLPMVSAILAHKT
                     HAPNITMVYESGGQNPIEGDMPWSVGDPFTWRKSPMIQVMAYSFAQSYNGYVDIAFLG
                     FAQIDCYGNINTHMIGDDFHKPKARLTGSGGNNDLSSLTENMVLVGIQSPDKFPKKLD
                     FITSVGHLTGGDSRKKAGMLGKGPVAVVTNAGVYDFEPVSKRMRVKSLHAGVNFDLAQ
                     LTTGFELLKPEGEIPVTDAPSDEALHILRTEVDPHGVFTSIPGM"
     gene            8683..10005
                     /locus_tag="PKJ16_04880"
     CDS             8683..10005
                     /locus_tag="PKJ16_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46356.1"
                     /translation="MSEIDTHARVHDLLLKNGIAAITEPVSFRKSIASNGTRSAVAVS
                     VKDFEKRYTISENGVMALAEGTEEAFRKHIDAQFPDDDALRRFRDDIATTGVKYGVPH
                     TFIVSDGAATIVVDPACKKRRLADIAAHGVLPGDSIILCSHHHPDHWANAGRIARGAM
                     CIVFHPDTKAILARGFFRFCYGEYLRILRTSPAGDIFPRVLGIGGIGRRIARFMLSRL
                     HRLTALGIYVITARIYGKTGMRRASLRFLEWEESSDFVFGATTVRGWRLSENCVALHT
                     PGHAPDTLSLLVRDRRALFIADSSAFLNPVSDHDGTIAGVHENVARLLAIVTSEKIEI
                     LLPSHAEPVVGHAAILEFLRSYDASLREVESLFGGIIAARAEWRFGELADTIWRHDHP
                     LARDLVKSNWPRTMSNIDLYLLMLLPEIGYRPLDSRRERWRRESAIRQ"
     gene            complement(10027..11013)
                     /locus_tag="PKJ16_04885"
     CDS             complement(10027..11013)
                     /locus_tag="PKJ16_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46357.1"
                     /translation="MKPGASFPWPSERVREYVLANTRTVEVTGHYLSRIGIGLILALA
                     RSFSWEGADRAGAFFGRLMYLLRIRWSTARVNLDIVYGERKTMKEKRAIYRESLVMVC
                     RHFANFLRVPLMDEEFLRTRFEIENEELLRDAYNQGKGVILVGGHIGEWDTAAGRIGM
                     ANYPIAIVAKKFKNPVMDKVIVDARCGMNLGTIAHVNSMQRIREGLARGEGVIMAMDQ
                     NMKRSQGMFVEWLGRPACTVRSNAWVARETGAPVICGYACRVAPGRFKLILTEEIPWE
                     PCPDDRERELAINTRNQVKAIEKIILAHPELWFWIHRRWKTQPEGVPSPYGD"
     gene            complement(11017..11595)
                     /locus_tag="PKJ16_04890"
     CDS             complement(11017..11595)
                     /locus_tag="PKJ16_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46358.1"
                     /translation="MEHYRHTTYFAKKKFLKLFGGEIKIFDQTKTQVLFFVKQKAFKL
                     KEDITVFSDESMATPLLTIKARSIIDFSAAYDVTDATTGEKVGALRRRGFKSILQDSW
                     ELMNAVDAVVGTVTEDSMLMAVLRRFLTNLIPQSFTIEMGGAPVGILKQTFNPFVPQF
                     NVDFTMDAGNRLDRRMGIATVILLQIIEGRQN"
     gene            11773..14544
                     /gene="ileS"
                     /locus_tag="PKJ16_04895"
     CDS             11773..14544
                     /gene="ileS"
                     /locus_tag="PKJ16_04895"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HNV46359.1"
                     /translation="MDYSKTINLPETDFPMKANLAQREPEMLKRWEAAGLYTAILASR
                     DGAPLYVLHDGPPYANGNIHMGHALNKILKDVIVKHKTMMGFRAPYVPGWDCHGLPIE
                     LHVTRELGEKAKELAKIDIRRRCRKYADKFVKIQMAEFKRLGVFGNYENPYLTMAPAY
                     EAKIVETFGNILKEGFIYKSKKPIYWCPTCVTALAEAEVEYDNHTSPSIYVKFKVDPS
                     SFHADGVDAHNLFVAIWTTTPWTLPANLAICFHPEFEYSVFRAGKEFLIMADGLAEQM
                     SSITGLALEDKRPIAKDSIENLKVSHPFIKRESKVIFGDFVTLDAGTGIVHIAPGHGQ
                     EDYVVGLKYGIDTLSPVDDEGRFTDEYAAMKGENVFDANPKIVQMLKDTGVLIFTNAI
                     EHSYPHCWRCKKPLIFRATEQWFMKIDHRDMRQTALAETDRVVWIPAWGAQRFRGMVE
                     SRPDWCLSRQRSWGVPIPSFRCKKCQANLMTAETVLHFAKLSLTKSIDAWYSEDIANL
                     VPAGTTCSCGGTEFEKEFDILDVWFDSGVSHFAVLDEWKELRWPSDIYLEGSDQHRGW
                     FQSSLWPAIALRQRAPYHTVLTHGFLLDDKGKAMSKSEGNVIHPEEIIKKYGADILRL
                     WVSSEDYRNDVRIGFEMLNQVADSYRKIRNTFKFIIGNLSDFSPDKAVPYAQLADIDK
                     WMLHKLYHLANQTMESYEKFEFHMVYRRILNFCAVELSSIYFDISKDILYIEAKDAPS
                     RRSNQTVLAEVYHALVRLVAPVLSFTAEEIWQFNKNTDSIHQQVYHPLRDEYDNPAID
                     EKMERLVEIKTDALKALELKRKAKEIGTSLEAELVIHAKSASARELLAGMADITRFFQ
                     VSKVTLAAEKAEGMSEYDNSFIAVAKSAGVKCVRCWNYFDRAQIGANPDHPELCARCT
                     DIITRSSHE"
     gene            14537..15043
                     /gene="lspA"
                     /locus_tag="PKJ16_04900"
     CDS             14537..15043
                     /gene="lspA"
                     /locus_tag="PKJ16_04900"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNV46360.1"
                     /translation="MNKHAIAGIIFAVSLIADILTKWLVVSKIALHERIDVIGSFVQL
                     TLVYNRGGLFGILQGYQAVFLVVSLVVLAIMIAFYVFEKNKTQLFCVSMALIASGAIG
                     NISDRFIGMLGIADRPGVVDFVYIGSDAVFRWPAFNIADAAIVVGAGLLLIVFFVEEK
                     RRKRSGAA"
     gene            complement(15040..15423)
                     /locus_tag="PKJ16_04905"
     CDS             complement(15040..15423)
                     /locus_tag="PKJ16_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46361.1"
                     /translation="MKQPIAIALLVALALAGCKKAETLSFCEGMSPEGKTVNCGTEFS
                     QGDLSAVITSKEPFGVSTLRVTVLEKKKFKDEAVKSFDVEVKADESMKAFDLSFYNDG
                     EYRVTVSGKENSVIGENEIRIIDTY"
     gene            complement(15420..16406)
                     /locus_tag="PKJ16_04910"
     CDS             complement(15420..16406)
                     /locus_tag="PKJ16_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HNV46362.1"
                     /translation="MKQPSGASAYLDVAREVLTVESKAIARLAGSLDENFVRAVERIA
                     KCRERVVLTGMGKSGLICRKIAATLTSTGTQAGFLHPSEAMHGDLGIIKKGDVVIAVS
                     NSGETREIIDIIPHVKILKATIIAMTANAESTLAQESDIPLIYAIEREGCPLNLAPMA
                     STTTVLALGDALAAALIHAKKFTAEDFNLYHPRGRLGQILLKVRDLMVAERLPVVRRE
                     TSMREVLSIMVSSNVGAVFVAGTRGHLRGIISDGDIKRLIERDEQFMRRTAGEVMNAT
                     PAWIYEDALAREALIRMEERRITVLPALDRRKRIVGVIHIHDIIKHNYGKTK"
     gene            complement(16403..16699)
                     /gene="xseB"
                     /locus_tag="PKJ16_04915"
     CDS             complement(16403..16699)
                     /gene="xseB"
                     /locus_tag="PKJ16_04915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010131684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HNV46363.1"
                     /translation="MEKKKKASPALSFEKALEGLEEIARRLEDGELDLDESIAEFEKG
                     VRLARICHEKLDAAEKKIEILQKAEGGRIERRAVDTDEETGELDDDEDIQGTLL"
     gene            complement(16677..17975)
                     /gene="xseA"
                     /locus_tag="PKJ16_04920"
     CDS             complement(16677..17975)
                     /gene="xseA"
                     /locus_tag="PKJ16_04920"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HNV46364.1"
                     /translation="MNDRFYTISEFTRLIKATLTGTPELNQVWLKGEITNLTYHSSGH
                     IYFTLKDEGAVISSVFFKYANRSMAFRLEEGMSVFAFGSVGLFEKRGSYQFTVSQVRL
                     EGAGELQKRIEQLKKKLLAEGLFDPARRRRIPFLPRRIGVVTSPTGAALRDIIKVATR
                     RFPNVEILIAPALVQGAGAADSVARGIEELNDPSHGVDVIIAGRGGGSFEDLMPFSEE
                     VVVRAFANSRVPIISAVGHQVDHPLCDDAADAAAPTPSAAAEIAVPVKVDLQERIDYC
                     RARAYSAMLSYVREATLRIDAATSVRVFRTPRDIVETRMALVRERERGMIVALERRVA
                     EKRRRFLSVPDVRRLAAAQVARTEMRLAVASEKLQKLSPLGTLRRGYAVARGADGKIV
                     RSVKMVAVRDRIDVILRDGALTCTVDSISRRVIGGKEEKG"
     gene            complement(18033..19025)
                     /locus_tag="PKJ16_04925"
     CDS             complement(18033..19025)
                     /locus_tag="PKJ16_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46365.1"
                     /translation="MKRLGVVLLFLVCGAGFASCKQNDLFPDAQKTESATQFKGDPVF
                     VAVGANGTIIYSGDGITWSAGTSGAGGSDVLYAVSSNGKKTFVAVGMGSFVRFSEDGG
                     NTWIPGSGPLVIESAVASDGDRGWVVGNGINDLRISNDNGRTWYDPAVTLGSTVNSLI
                     YNAALNRWILLCSGGSYAQYNDDWFTTGATNASGVTSMMRAVVYANSQFIAVGNGPDA
                     FHSSNGATWAVGSTGMPIAPAYMKDIDYGNGRYIAVGDNGGTGMAYYYTGSGSWNNTT
                     FSGTVLQGVAFGNNVWVAVGTGGSVYYSLDNGDNWIPATSGTPQNLNGVAFGYR"
     gene            complement(19262..20173)
                     /locus_tag="PKJ16_04930"
     CDS             complement(19262..20173)
                     /locus_tag="PKJ16_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV46366.1"
                     /translation="MRTFHAGNRDALFMGLMALAMAVWGGSWVCAKALGNGIHYQKLA
                     FVRFTLTVLFFVPPIIALRESLRIRPATAGLILLGSVLYTLYSQMFFLGLSLGTAGLG
                     GVLVTSLVPIITYAGVSVLGRKKLRVIDAAGLAIGCAGALVILQIWRFSHEALLASGN
                     LFFVIGAALWSGVTVNSQRTQSSVSIWVYSFYLNGFAALIRFLFVLPYGLDGLVSAEP
                     SWWFSMTYLSLFSTVGATSLYFYAAKKIGSRRASAFTFMVPLTAVLLSWVVLGEVPAV
                     STIAGGLLSIGAVYLIQFGGAAETPGA"
     gene            complement(20206..21372)
                     /locus_tag="PKJ16_04935"
     CDS             complement(20206..21372)
                     /locus_tag="PKJ16_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46367.1"
                     /translation="MIPVPRLAIASGTCAFALAAIILACGSPQKTARPVRTLMIPPFQ
                     NQSGVEANDHLAVGVADELARRMQASGIFRSIDPAGLFDRLTISQVYGTAKEEQQLLG
                     ALRTLDTDYAVLGTVSTADKGKTMTITAKVIRSRDNTVLASEREEGPPANWTELRGLL
                     AIKIAKRLGIPLTAQEKQQLVVGETRDHEAFRLNYIGKMQYYRSLEARYRSREVEAER
                     YRNIAMAYWSKSIERDGRYEAARDNYARAMDVGDVRSSLDARVERAQGSLNGMIMYGF
                     LYKSSKVWDPTAWVRANADVLRAMANTLPQGYVIEVQGHTCCSGPEEPRDGKPGNIRI
                     SDDRAREVYQAMVGIGIKGSLLRHKGYGSSRTIPWLDPRDQSNRRVTFEVKPGL"
     gene            complement(21399..22553)
                     /locus_tag="PKJ16_04940"
     CDS             complement(21399..22553)
                     /locus_tag="PKJ16_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46368.1"
                     /translation="MKRSVRMQFLIWCAVVAVASASFSCRDAGTQAGDEQRISIIIPP
                     FVNTTVAGNRDDYDYLSIGIADELSNRLAYSDRFDVIDPALLVEKITLRQLVGGAKDE
                     KALVDAFLGLKAEYAVMGTVSLSVDRNVLTLSARALRTADRKTMAEATETGEASEWNH
                     LRGLLASRLAAKLGVKLSGTALRQLAEADTREFAAFRENYIGKLYYYKSLEAGINKKE
                     DDVRKFKQLSLLHYSKAIARDVNYQTVRKNYVQLFDFKKLLSTKEEKSARIQGRLNEM
                     TIFGIANQADANRHIAEWAAKNQVLIKSMINGLPDEYRLEIQGHTDSAGSPALAAARA
                     KQVYDALRGVGVFDFKMTHKGYGSLKRIPWIHPGDKSNNRVTFAIVSVIK"
     gene            complement(22643..>23320)
                     /locus_tag="PKJ16_04945"
     CDS             complement(22643..>23320)
                     /locus_tag="PKJ16_04945"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HNV46369.1"
                     /translation="LFDVVIQLGAILTVVVYFWRRLIPFGGGITADERKRVFGIWLKT
                     IVGVLPALAIGFFAAKAIKEYLFNPVTVAIALIAGGVVLIVLERRARESSITSMDELS
                     FKTAFLIGIIQCLAMVPGTSRSAATIIGAMLLGSSRVVAAEFSFFLAIPTMVAASAYS
                     MLKAEKALGAHELTVLGIGFAVSFVVALLVIAIFMRFITRHDFKPFGWYRIVLGIAVL
                     AYFLLIK"
BASE COUNT         4476 a         7473 c         6906 g         4465 t
ORIGIN      
        1 atcatcaaac actaataaga cacgaaatac aggcaatggg accgtcctca tgtatgcctg
       61 ctgacacggc caagacgaac tcgctgtcgc aggtctccaa acggtttttt aatactcgag
      121 agcgttgtaa atgttctgat ggtcgcgacg cgttatgagt actctgaggt ggtctcgttc
      181 aaatcgaaac aaagaatgga gtaggccatg ccggacaata atagcgagga tccgattgta
      241 cgcacgaccg cggacataga gcgccgcgca gtcgcccacg gggcggcggt gcacccgagc
      301 aggcatactg ctcatgaaca agaaaaacac tatccacgta tggtaattat cgttctctcc
      361 gatgtcgcac acgcccacgc atcagaaaca tcggtcatcg aacttccccc ttcacgcgcc
      421 aaggtgaaaa atctctcaaa gaggaaagga ttcgggagta cacacggccc tagaaattca
      481 ccaccggccg catctcgatc tccggataga tctgcccctg cttgggcggc atggagttgt
      541 acacccagtc gatgtccacc gtgcccagtt tcacgccgcg gtgccgcgag aagccgcgga
      601 aggtgccgcg cacaagcacg tcctccatgg tgagcgaaag accgaacacc gcgaccgtcg
      661 ttccggcggg aatggccgcc agttcttccc ggtaggccgt gggggcgaag gcgagccgcc
      721 ggctgtcggc cgcctggcac tggatcgccg ggatgatgac gggatagccg tccgagccga
      781 tgtacgatat gaacttgagc gcgccgatat cattgaaaag atcctcggcg aacggcttga
      841 ggatgcgcgc ctgttccccg gatgacgcgc cggccttcgc gcatttcgtc atgagcgcgc
      901 cgcggacgat gccgcccatc gggagcttct cgcgtccggt cgtctctacc aggtccatgt
      961 agtgcaccgt gtggaggccg aagtacgagt tgtaccgcca catgggcttc ttgttgaaca
     1021 tgatgtactc gtcgccctcg ttcagcgcct tcttccagac cgccttcccg cgccagagct
     1081 cctttgataa actcatgata agaaagccca cgcgcggatt cttccgtacg ttccgcttac
     1141 tcatcccctc ggagaactgg ccccagatga gctccgtggt ggagcgcgcc tgcagcgccg
     1201 tgatcaacgt tatgtgcggc agttcgcctt cgctcaccgt ggcgatgagt cccaccttgg
     1261 cgtcgggctc gaattccttg aggtcgttcg gatcgaatac gttaagtttt ttcatggttt
     1321 cctctcatgg ttgatgttac ctcaccccgc ctccggcgcg ccctctcccg caaggagagg
     1381 gctctgcaat agtcaactat tgcctctccc ccttctccta tgggagaagg gggttggggg
     1441 gatgaggttt tcagcatccc ttcactagcc gcatgtgcgc cttcgcgtca ttgtccgcct
     1501 gctcatcatc gcttatgaac ttcgtcgcac cgctccagtg cacgatcacg ggatcgacgc
     1561 cggaatggcg cgccacgttc gcaagatgct ccatcgtctc cctgccgagg agcgcctcag
     1621 ccccaaagtc ccactcgaga tcgagacgcc gatacttgtc gcggcagaga ttattgaagg
     1681 cgcacagttc ccagcgctcc accgcgcctg cgatgttctc cgcgatgtag gcgccgattc
     1741 ctgcgatgtt ctcgtcacgc gcggtcgcgt tcgggatgat gggcgtgcgt atccacagtt
     1801 tcgtcttcga tcccgttttc gcgatcgtgt cacggacgtg cttcaggttc tcgagcatcc
     1861 tctcgttgcc gctgccggtg aatctcttgt gcaacaccgg atcgatctcc ttgatgtcgt
     1921 agagcaccag gtccgcatgc ggcaaaatcg cgtcgaacgc ttcctttcgg cagagaccgc
     1981 aggtatcgag cgcggtgtgc agtccgcggc gggtgcattc gcgcaagaac gccgcggtaa
     2041 actccggctg catgcagggc tcgcccccgg atatcgtcac gccgccgccg gagctctcga
     2101 agtacgagcg gtccttttcc acctcgtcgg ccagttcatc gacgccccac tcggttccca
     2161 agagctccat cgcgagcgcc gggcactcgt ccacgcagat gccgcagagc gtgcatgcgg
     2221 cgcggtcgag cgcgatcacg ccggcatcgt cggagagcgc ctgctgcgga caacccttca
     2281 cgcaagtgcg gcacccgatg cacttcgtct cgatccactg gatttgccgc tcgaacggaa
     2341 tgctctccgg attgtggcac cacgcgcagg cgaggctgca gcccttcacg aagaccgtcg
     2401 tccgaatccc cggcccgtcc tccgtggaca tccgctggat gttcagaaca agccctttga
     2461 ttgcatctct ttcgcttttc atccgcttat ttcgatcgta cttattaccg ataatcccat
     2521 caacacgcta actcattctc tcccatccct ccccctaacg aagggggagg tgcccgaagg
     2581 gcggagaggg tcaataccga ttatggctct cccgggcgat gatctcgtcc tgcagctcgc
     2641 ggccgatcgc cgtgaagtag gcgttgtagc cggtcacgcg caccagcagg tgccgatagt
     2701 tctgcgggtg ctgctgcgcg tcgcgcagca tgtccgcgtc gatcatgttg atctgcaacg
     2761 ccgtgccgcc gtttttcgca tagccccgca ggaaggcctt gaatttcttg cggtgctcgg
     2821 gatcgcgcag gatcgacgga ttgaaggtga tcgtgtggct cgcgccgttc gggagattgc
     2881 tcaggtagcc ttcccaatca gccgcgccgc cgtccgccgt tcccccgagc acctttccca
     2941 cggaattcgc attggccgtg gggccgttga tgtccgcgcc gttcgaaggg cagatcgcgt
     3001 tggagaggaa ctgccccttg ggacggccgt cggggctcgc ggccatcacg tagccgtcgc
     3061 ccacccagta gttccacgag agcatgcccg gccggaactg ccggttggtc gatttcgtct
     3121 tgtactgcca ggtctcctcg gcccagaggt ccatcaccgc gagcgccatc gcgtcggcct
     3181 cgttgtcgtc gcggccgtac ttgggcgcac ggttcttcgc cttggcctgc aggatatcgt
     3241 ggccctccca attgtcgcga agcgcctgga tgagctcggc catcgtgcat tccttcttat
     3301 cgaagaccag gtacttgatc gccagaagcg aatcgaccgt cgtgccgaag gtgacgccct
     3361 cgatggtcac aaacgaaagc tccgcgccgc cctgcgtgat gtccagcgcc ttctcggcac
     3421 agccccccac caggcacgag agatacggcg tgggacagaa ctcagctcga atgcgctccg
     3481 acgcctcgta cacctcgacg cagcggcgga tgatgttttt cgtatggatc acgtaggcgt
     3541 tccagaattc atcccatgtc gaaaacttcg tcgcgtctcc cgtcttcggt ccgtcctgcc
     3601 tgatgggatc gcgcttcccg gtgatgggat cgacaaactc gaggaggtcc ttcccgccgg
     3661 tgagcgcgag ctccacggcc ttgagaaggt tcaggttatt gtccacggtg ccggatcggt
     3721 cattgccgac catggtgttt tccaggcagc cgaccggcgc atactcgtgc acgttgtttt
     3781 cgttgatgag gtgcgccaca ttcgcgcgct tcgcctcgcg catcatcccc gccatggagc
     3841 gctcgtcgaa attaagcaga aacggcgctc cctggctgga cgaaaccatg tcgatgagcc
     3901 ggtcgaggag ctcgtccggc gtattgcggt gcatgcggac attcggcttg ggctccagga
     3961 tcggcgtcat ctcgtcgatc acctcgagga ccgcataggt gagctcgttg gtcatgtcgg
     4021 ccccgttcgc gccgaggccc gaaagggtga tgagctgccc gaagcccgac gtgataccct
     4081 ggttcccgac ctgcaccatg gcgtcgtagg ccgtattgct gtggatccag aagcacttga
     4141 ggatctcctt cgcgaaatcg cggtccatgc cgtccgccat ggattttttc caatacggca
     4201 gcaggtactg atcgaaccgg ccgaaggaaa cgcccggccc gggatagttc tcgtcgctca
     4261 tgataagcat gtggttgatc cagagcgcct gcacggcctc ccacaatgtc tcggccggct
     4321 cccaggggac tcggtcgagg tttttcgcca tttgctcgag ttcgcttttt cgcccggcgt
     4381 tcttttcctt cgcggagagc ttccggcaga gcgcggcgta cttcgcggca aggtcgcgcg
     4441 gcattgtcgc cgcggtcatc atcgcccgca gctgcgcgcc cttccttcct tttttgtctg
     4501 attcggacag cgccttgtat ttttgctcca ggtcggcatg gatccccttc cagccaatcg
     4561 tgagcgcgcg ctcgtgcccg gggaccaggt gcccgctcgt cgcaccggag tttccatacc
     4621 cgtgatcgtg gaaccacttc atgaccgcgc gcgctccctt cgttccgtag atgagcgcgt
     4681 cgcgcgcctt cgcctctttt tcgctccagc agcgcgaggc ctgcagattg aagcgcgccc
     4741 ccgcgatgag atctcccggc aatagttctt gaggcacgca attcaccatc acctctttcg
     4801 tgaaccacgc cttgcgctcc ggaaggctcc agctccagaa atcgggatgg agcgttacca
     4861 cgcgcgcgct ctgccggaac gaggaccgga aggtctggtt gaatgcgtac atctccggga
     4921 cgatgtagta cgagatctcg tcgaactgga cgtcccagtc ggtgccggtg gtccaggcgc
     4981 tgtactcatt gttccacgcc cgccgtgcgc ccacgaaata gtagtcgcgc aaccactgga
     5041 tgcgcggaga gaggtttttc ggttccttga tcttgaatgc gggtgctgtc gatgctgcgc
     5101 acatggcgtc ctcctgatga atggtgctgc gttgtacgat ggggcacggg cagtgtccgt
     5161 accctacaat tatatatttg aaaccaatcg aatcttccgg ttctcgatgc cgatgcccag
     5221 ggcgtccacg acgcgcgcct gcccgtactc cagcgcctcg cgcatgatcc gctccgcctg
     5281 atgcgcgtct ttccggcagg ccgcatcgac gaaacgccgg tgctgttcca tcgtgatgcg
     5341 aatcgcctcc ggcagattga aaaagaaatc cgtatagaag cgatacaccg gccggaacga
     5401 attaaagacg agctggatga tgatgttgcc cgaccgcttc atgatgagtg cgtgcagccg
     5461 gtagtcgcat tccgccatcg ccgcgggatc ggacagcgac cgctcctcga taccgatgat
     5521 gtcgcgaagc tcgcgttcgt catccggcgt gaaggcggcg accgccatct tcaccgcgtg
     5581 gagctcgatg agcaatcgcg tctccatgag cgcgcgcagc gtgtccgaat cgacctcgcc
     5641 gccgttctgc atgatgtcga tgagcacatc gagcgacgcc ttcgcctgat agtcgctcac
     5701 gtaggtgccg ctctgcgggc gcgtctccac gaggccgctc ttggcgagcc tgccgaccgc
     5761 ctcgcgtacg gtggagcggc tgacgccgaa ttcctccgcg agatcgcgct cggcgggaaa
     5821 gcggctgccg gcgggcatat cgcccacaat gatggctttt tggagctttg tgaagattgt
     5881 ttccgaaagg cgatccgaca ttattttctc cggaataatt ggtctgacca attatcaaca
     5941 taccgataac tattcgaatc ggcaagccaa tttcagtaaa aaatgctgat aattgataaa
     6001 taatattttg caaaataaac attcgaagat aaaatgtcca tatcgaagcg ataactatcc
     6061 gtttcacggc atcaaggagg atcgacaggc agatgtgagg taatgacaac cggcttccag
     6121 gcaccaccgt ccgtacgctg atgatgaggg tcacggccgt acagagaccg tgtttggaga
     6181 ttcccgaata gggggcctgg cgttcgaaag aacgcacttt tccacgagga ggggctcact
     6241 atgggtacat tcagttacaa tccggaagcc atcgccaacc tcaagatcac cgacacgtcg
     6301 atactcgacc gtctcgtcga tttcgaatcg gcgcgcgcgg cgcacctggc gaaggaccac
     6361 tcgcggcgcg acaagcggat gtcgctttcg gaagcgatct ccgcgttcgt cgccgacggc
     6421 gatatcgcca ccgacgccgg cttcgcctat gtccgcacgg cccaccaggc ctatttcgag
     6481 atcatgcgcc agggcaagaa gggcctgcac ttcatcggct cgcccaacac caaccagagc
     6541 tacatgatca acttcggggt gtgcgcgtac tcgcacaact cctacaccgg cgcggagatg
     6601 cgcggcaccg accgcaacta ctcgcgccag ctcaagaacg gaaaagtgaa gatcctctcc
     6661 gagtggagcc acggatcgat ggcgcagggc ttcaaggccg cgcagctggg cgtgccgggg
     6721 gtcttctcga agcagctcct gggatcggac attctccgtt acaatcccta cgtccgtgtg
     6781 atgcaaaacc ccatgcgcga cgatcccgac cccgtcgtgt tcatccccgc gctctatccc
     6841 gacgtcaccg tcatccacgt gcacgccgcc gacaagaccg gcaatgcgcg catctacggc
     6901 ccgacggtca acgacatcgc gctcgcggcc gcggcgcgca aggtgatcat caccgccgag
     6961 gagatcgtcc ccgagatcga catccgctac aacagcaagg gcgtggcgat cccgttcatg
     7021 tacgtcgacg ccgtggttga gatgccctac ggcgcggtgc ccggcaacat gcccggccac
     7081 tactactggt cgcgccagtg gtgggagaag gccttccgct acgcctgcgt ctccgacgat
     7141 aacatgaagg ccttcatgga cgaatgggtc ttcgggtgca aggaccagtt cgagttcgtc
     7201 gacaagctcg gcggcgcgaa atggatcacc atggcgcgcc gcctcaccaa ggccgccgag
     7261 tacgacaacg agagcgacgg cgtcgatttc agctatcagc cctatctgcc ggacaaggat
     7321 ccggggatct actattaaga ggaggcacga tatgacaacc aacaaaccgg caaacccgct
     7381 cgagatggtg gcctacgtcc tctcgcggca gatctcggat caccaggtcg tgtacgtggg
     7441 gacgggcctc ccgatggtct cggccatcct cgcgcacaag acgcacgccc ccaacatcac
     7501 catggtctac gaaagcggcg ggcagaaccc catcgagggc gacatgccct ggtccgtagg
     7561 cgatcccttc acctggcgca agtcgccgat gatccaggtg atggcctact cgttcgcgca
     7621 gagctacaac ggctatgtcg atatcgcctt tctcggcttc gcgcagatcg actgctacgg
     7681 gaacatcaac acgcacatga tcggcgacga ttttcacaag cccaaggcgc gcctcaccgg
     7741 ctccggcggc aacaacgacc tctcgtcgct cacggagaac atggtgctcg tgggcattca
     7801 atcgcccgac aagttcccga agaagctcga cttcatcacg agcgtggggc acctcaccgg
     7861 cggcgattcg cggaagaagg cgggcatgct gggcaaggga ccggtcgccg tggtcaccaa
     7921 tgccggcgtg tacgatttcg agcccgtgtc gaagcgcatg cgcgtgaagt cgctccacgc
     7981 gggagtgaac ttcgatctcg cgcagctcac gaccggcttc gagctcctga agcccgaggg
     8041 ggagattccg gtgaccgacg cgccgtcgga cgaggcgctg cacatcctgc gcaccgaggt
     8101 cgatccgcac ggcgtgttca ccagcatccc gggcatgtaa tgcgctcacc ggcggccgtc
     8161 cgcgcgggcg gccgccggaa tcatcccgcg catacctgca tactgctccg gaagggtttt
     8221 tcgcttgaaa aagccgtcgt acagtgcaca catagccgtg atggccaacg gaagaaaaaa
     8281 atcatatatc cgaaatccct gaaggatatc ccgcgctggc ggcgacgcga gcttgccgag
     8341 aacctggagt ggtttctgcg cttttcaccg gtcgagcgcc ttcgctacat cgaccgggaa
     8401 tgggaagaga catgcacgta tatagagaaa tacagtctgg ttaagcgatg gaaggcgaaa
     8461 aagaactaac cttgaaatcc gtaattgcaa tgataaactg atgatttttc agaatgctat
     8521 gcgaattcgg acaacattga agaccttcag gagcgactgg tgttccgggt cccctcaatc
     8581 gatgacctga tacgactgaa acagattcga gaaccaaaca gtaaagacgc gcaggatatc
     8641 gagtacctcc tgaaagcgaa acaatcccga aaaaaacacg ccatgagtga aatcgacaca
     8701 cacgcacgcg tccacgacct cctgttgaaa aacggcatcg ccgcgattac ggagccggtg
     8761 agcttcagga aatccattgc ctcgaatgga acgcgcagtg cggttgccgt ttcggtgaag
     8821 gatttcgaaa aacgatatac gatcagcgaa aacggcgtga tggcgctcgc ggagggcaca
     8881 gaggaagcct ttcgcaaaca tatcgatgcg cagtttcccg atgacgacgc cctccggcga
     8941 ttccgcgacg atatcgccac gaccggcgtc aagtacggcg tgccccacac cttcatcgtc
     9001 tcggacggcg cggcgaccat cgtggtcgat ccggcgtgca agaaacgccg cctcgccgac
     9061 atcgccgcgc acggcgtcct tcccggcgac agcatcatcc tctgctcgca ccatcacccc
     9121 gaccactggg cgaacgccgg acgcatcgcg cgcggcgcca tgtgtatcgt ctttcacccg
     9181 gacacaaagg caatcctggc gcgcggcttc ttccgcttct gctacgggga gtatctgcgc
     9241 atccttcgca cctcgccggc cggcgacatc tttccgcggg tcctggggat cggcggtatc
     9301 ggccggcgga tcgcgcgatt catgctttcg cgcctgcacc ggctcaccgc gctgggcatc
     9361 tacgtcatca ccgcgcgcat ttacggaaag acgggcatgc gccgcgcgag cctccggttt
     9421 ctcgagtggg aggaatcgag cgattttgtt tttggcgcaa cgacggtgcg cggatggcgc
     9481 ctgagcgaga actgcgtcgc gctgcacacc cccggccatg cgcccgatac gctctcgctg
     9541 ctcgtgcggg accgcagggc gctcttcatc gcggacagca gcgccttcct caacccggtc
     9601 agcgaccacg acgggacgat cgccggcgtg cacgagaacg tcgcgcggct cctggcgatc
     9661 gtcacgagcg agaagatcga aatcctcctg ccgtcgcatg cggagcccgt cgtgggacac
     9721 gcggcgatcc tcgagttcct gcgctcctac gatgcgtcgc tccgcgaggt ggaatcgctc
     9781 ttcggcggca tcattgccgc gcgcgcggaa tggcgcttcg gcgagctcgc ggacacgatc
     9841 tggcgccacg accatccgct cgcgcgcgac ctggtcaaat cgaactggcc ccggaccatg
     9901 tcgaacatcg acctgtacct gctgatgctg ctccccgaga ttggctaccg gccgctcgat
     9961 tcgcgccgcg agcgctggcg gcgcgagagc gcgatcagac aataactgaa acgagcgtca
    10021 cgcgcatcaa tccccgtacg ggctgggcac gccctccggc tgcgtcttcc agcgacgatg
    10081 gatccagaac cacagctcgg gatgcgcgag gatgatcttt tcgatcgcct tcacctggtt
    10141 gcgcgtgttg atggcgagct cccgctcacg gtcgtcgggg cagggctccc aggggatctc
    10201 ctcagtgagg atcagcttga accgtcccgg cgcgacgcgg caggcatagc cgcagatgac
    10261 cggcgcgccc gtctcgcgcg cgacccacgc attcgagcgc accgtgcacg cgggccggcc
    10321 cagccactcg acgaacatgc cctggctgcg cttcatgttc tggtccatgg ccatgatcac
    10381 accctcgccg cgcgcgagcc cctcgcggat gcgctgcatg gaattcacgt gcgcgatggt
    10441 gcccaggttc atcccgcagc gcgcgtccac gatcaccttg tccattaccg ggttcttgaa
    10501 cttcttggcg acgatcgcga tggggtagtt cgccatgccg atgcggcctg cggccgtgtc
    10561 ccattcgccg atgtgccccc cgacgaggat cacgcccttc ccctgattgt acgcatcgcg
    10621 cagcagctcc tcgttctcga tctcgaagcg cgtcctgaga aactcctcgt ccataagcgg
    10681 cacgcgcagg aagttcgcga agtgccggca caccatgacc agcgactcgc ggtagatcgc
    10741 ccgcttctcc ttcatcgtct tgcgttcgcc gtagacgatg tcgaggttca cgcgcgccgt
    10801 gctccagcgg atgcgcagca ggtacatcaa tcgcccgaag aaggcgcccg cgcgatcggc
    10861 gccctcccag gagaacgagc gcgcgagcgc gagaatcagc ccgatcccga ttcgcgagag
    10921 gtagtgcccc gtcacctcga cggtacgggt gttggccagc acatactcac ggacccgctc
    10981 ggacggccag gggaaagatg cgccaggttt catcggtcag ttctgccgtc cttcgatgat
    11041 ttgcagcagg atcaccgtcg cgattcccat ccggcggtcg agacggttcc cggcgtccat
    11101 ggtaaaatcg acgttgaact gcggaacgaa tgggttgaag gtctgcttga ggatgccgac
    11161 cggcgcgccg cccatttcga tggtgaacga ctgcgggatg aggttcgtga ggaaccggcg
    11221 caggaccgcc atcagcatgc tgtcctccgt tacggtgcct acgaccgcat cgaccgcgtt
    11281 catgagttcc caggagtcct gcaggatcga cttgaatccg cgccgcctga gcgcacccac
    11341 cttctcgccg gtggtcgcat ccgtcacgtc gtaggcggcc gagaaatcga tgatgctgcg
    11401 cgccttgatc gtaagcagcg gcgtcgccat cgattcatca gaaaatacgg tgatgtcctc
    11461 tttcagtttg aaggcctttt gcttcacgaa gaacagcacc tgcgtcttcg tctggtcgaa
    11521 aattttaatc tcgccgccga agagtttcaa gaatttcttt ttcgcgaaat aggtcgtgtg
    11581 tcgatagtgt tccatgatcc gctcccatgc gcgtccgatg tgacaagata gtgatgcgtt
    11641 actgttgcga gatagcatcg cgcgcggaaa attgcaatga taatactgcg ccgaaaaata
    11701 ctattgacaa aaacctcgcc cgtccggctc ttttagcaaa tttcgcgcac cgctggcgcg
    11761 cttggggctc acatggatta ttcgaaaacg atcaatctcc cggaaaccga tttcccgatg
    11821 aaggcgaatc tcgcccagcg ggagccggag atgctcaagc ggtgggaggc cgccgggctg
    11881 tacaccgcca tcctcgcatc gcgcgacggg gcgcccctct atgtcctgca cgacggaccg
    11941 ccctacgcga acggcaacat ccacatgggc cacgcgctca ataagatcct caaggacgtc
    12001 atcgtaaaac acaagacgat gatgggcttc cgcgcgccgt acgtccccgg ctgggactgc
    12061 cacggcctgc ccatcgagct ccacgtgacc cgcgagctcg gcgagaaggc gaaggagcta
    12121 gccaaaatcg acatccggcg ccgctgccgg aagtacgccg acaagttcgt gaagatccag
    12181 atggccgagt tcaagcggct gggcgtcttc ggcaactacg aaaacccgta cctcacgatg
    12241 gcgccggcct acgaggcgaa gatcgtcgag accttcggca atatcctcaa ggaaggattc
    12301 atctacaaga gcaagaagcc catctactgg tgccccacct gcgtgaccgc gctggccgag
    12361 gccgaggtgg agtacgacaa tcacacctcg ccgtcaatct acgtcaagtt caaggtcgat
    12421 ccgtcgtcgt ttcacgccga cggcgtggac gcgcacaatc tcttcgtcgc catctggacc
    12481 acgacgccct ggacgcttcc ggccaacctc gcgatttgct ttcatccgga gttcgagtac
    12541 tccgtcttcc gcgccggaaa ggagttcctc atcatggccg acgggcttgc cgagcagatg
    12601 agctcgatca ccggcctcgc gctcgaggac aaaagaccca tcgcgaagga ttcgatcgag
    12661 aacctgaagg tgtcccatcc cttcatcaag cgcgaatcga aggtcatctt cggcgatttc
    12721 gtgacgctcg acgcggggac cggcatcgtc cacatcgccc ccggccacgg ccaggaggac
    12781 tacgtggtgg gcctcaagta cggcatcgac accctttcgc cggtcgacga cgagggacgc
    12841 ttcaccgacg aatacgccgc catgaagggc gaaaacgtct tcgacgcgaa cccgaagatc
    12901 gtgcagatgc tcaaggacac cggcgtcctc atcttcacca atgcgatcga gcactcctat
    12961 ccccactgct ggcgctgcaa aaagccgctc atcttccgcg ccacggaaca gtggttcatg
    13021 aagatcgacc accgcgacat gcggcagacc gcgctcgcgg agacggaccg ggtcgtgtgg
    13081 atccccgcat ggggcgcgca gcgtttccgc ggcatggtgg agtcccgccc cgactggtgc
    13141 ctctcgcgcc agcgctcgtg gggcgtgccc atcccgtcgt tccgctgcaa gaagtgccag
    13201 gcgaacctca tgaccgcgga gacggtcctc catttcgcga agctctcgct cacgaaaagc
    13261 atcgacgcct ggtactccga ggacatcgcg aacctcgtcc ccgccgggac gacatgctcc
    13321 tgcggcggga ccgagttcga gaaggagttc gacatcctcg acgtgtggtt cgattccggc
    13381 gtgtcgcatt tcgccgtgct cgatgaatgg aaggaactgc gctggccgtc cgacatctac
    13441 cttgagggaa gcgaccagca ccgcggctgg ttccagtcct cgctgtggcc cgccatcgcc
    13501 ctgcgccagc gcgcgccgta tcatacggtg ctcacgcacg gcttcctgct ggacgacaag
    13561 ggcaaggcga tgagcaaatc cgaggggaac gtgatccacc ccgaggagat catcaagaag
    13621 tacggcgccg acatcctgcg cctgtgggtc tcctcggagg actaccggaa cgacgtccgg
    13681 atcggcttcg agatgctcaa ccaggtcgcg gactcctacc ggaagatccg caataccttc
    13741 aagttcatca tcgggaacct ctcggacttc tcgcccgaca aggccgtccc gtacgcgcaa
    13801 ctcgcggaca tcgacaagtg gatgctgcac aagctctatc atctggcgaa ccagacgatg
    13861 gaatcgtacg agaagttcga attccacatg gtctatcgcc gcatcctcaa tttctgcgcg
    13921 gttgagctgt cctcaattta tttcgatatt tccaaggaca tcctgtacat cgaggcgaag
    13981 gacgcgccgt cgcggcgttc gaaccagacg gtgctcgccg aggtgtacca tgcgctggtg
    14041 cgcctggtgg cgccggtgct ctccttcacc gccgaggaga tctggcagtt caacaagaac
    14101 accgacagca tccaccagca ggtgtatcac ccgctgcggg acgagtacga caacccggcc
    14161 atcgacgaga agatggagcg gctggtggag atcaagaccg acgccctcaa ggcgctggag
    14221 ctcaagcgca aggccaagga gatcggcacc tcgctcgagg cggagctcgt catccacgcg
    14281 aagagcgcga gcgcccgcga gctcctggcc ggcatggccg acatcacgcg tttctttcag
    14341 gtctcgaagg tgacccttgc cgcagaaaaa gccgagggca tgtccgagta cgacaattcc
    14401 tttatcgcgg tggcgaaatc ggcgggcgtg aagtgcgtcc gctgctggaa ctacttcgac
    14461 cgcgcgcaga tcggcgccaa tccggaccac ccggagctgt gtgcgcgctg caccgacatc
    14521 atcaccagga gctcacatga ataaacatgc aatcgccggc atcatcttcg ccgtctccct
    14581 catcgccgac atcctcacca aatggctggt ggtctcgaag atcgccctgc acgagcgcat
    14641 cgacgtcatc ggctccttcg tgcaactcac gctggtgtat aatcgcggcg ggctcttcgg
    14701 catcctgcag ggctaccagg cggtgtttct ggtcgtgtcc ctggtggtgc tcgcgatcat
    14761 gatcgccttc tacgtgttcg aaaagaacaa gacgcagctc ttctgcgttt caatggcgct
    14821 catcgcctcc ggggcgatcg gcaacatctc ggaccgcttc atcggcatgc tgggcatcgc
    14881 ggaccgtccg ggggtggtgg atttcgtgta tatcgggagc gacgcggtgt tccgctggcc
    14941 ggcgttcaac atcgccgacg cggcgatcgt ggtcggcgcg ggtctgctgc tcatcgtgtt
    15001 cttcgtggaa gagaaaagga gaaagcgttc cggcgcggcc taataggtgt cgatgatccg
    15061 tatctcgttt tccccgatca cggagttttc ctttcctgaa accgttacac gatattcacc
    15121 atcgttgtaa aacgagaggt cgaacgcctt catgctctcg tcggccttca cctcgacatc
    15181 gaaggacttc acggcctcgt ccttgaattt cttcttttcg aggaccgtca cccggagcgt
    15241 cgaaacgccg aacggctcct ttgacgtgat caccgcggag aggtccccct gcgagaactc
    15301 cgtgccgcaa ttcaccgtct tgccctccgg ggacatcccc tcgcagaacg agagcgtctc
    15361 ggcctttttg cagcctgcga gggcgagggc gacgagcagt gcgatggcga tgggctgttt
    15421 cattttgtct tcccgtaatt atgtttgatg atgtcgtgga tgtggatgac gcccacgatc
    15481 cgtttgcgcc ggtccagcgc cggcagcacc gtgatccgcc gctcctccat gcggatgagc
    15541 gcctcgcgcg cgagcgcgtc ctcgtagatc cacgccggcg tcgcgttcat cacctcgccc
    15601 gccgtgcgcc gcatgaactg ctcgtcgcgc tcgatgagcc gcttgatgtc gccgtcggag
    15661 atgattccgc gcaggtgccc ccgcgtcccc gcgacgaaca ccgctccgac gttggacgac
    15721 accatgatcg agagcacctc gcgcatggac gtctcgcggc ggacgaccgg gagccgctcg
    15781 gcgaccatga ggtcgcgcac cttgagcaga atctgcccca ggcgcccgcg cgggtgatag
    15841 agattgaagt cctcggccgt gaattttttc gcgtggatga gggcggcggc gagcgcgtcg
    15901 cccagcgcga gcaccgtcgt ggtgctcgcc atcggcgcga ggttcagggg gcagccctcg
    15961 cgctcgatcg cataaatgag cgggatgtcc gactcctgcg cgagcgtcga ttcagcgttc
    16021 gcggtcatgg cgatgatggt cgccttcaga atcttgacgt gcgggatgat gtcgatgatc
    16081 tcgcgcgtct cgcccgagtt cgagaccgcg atcaccacgt cgcccttctt gatgatgccc
    16141 aggtcgccgt gcatggcctc ggagggatgc agaaagcccg cctgcgtccc ggtggaggtg
    16201 agcgtcgccg cgatcttgcg gcagatgagc ccggacttcc ccatcccggt gaggacgacg
    16261 cgctcgcggc acttcgcgat ccgctcgacc gcgcgcacga agttttcgtc gagcgacccg
    16321 gcgagacgcg cgatcgcctt gctctcgacc gtgaggacct cacgcgcgac gtcgagatac
    16381 gccgatgcgc cggatggttg tttcatagca gcgtcccctg tatgtcttcg tcgtcgtcga
    16441 gctcgccggt ctcctcgtcg gtgtccaccg cgcgacgctc gatccggccg ccttcggcct
    16501 tctgaagtat ttcgatcttc ttttcggccg cgtccagctt ctcatggcag atgcgcgcga
    16561 gccggacgcc cttctcgaac tcggcgatgg actcgtcgag atcgagctcg ccatcctcga
    16621 gccggcgggc gatctcctcg agcccctcga gcgccttctc gaaggagagc gcggggctag
    16681 cctttttctt cttttccacc gatcaccctc cgtgatatcg aatcgaccgt gcaggtcagc
    16741 gcgccgtccc tcagtatcac atcgatacgg tcccgcacgg caaccatttt tacgctccgt
    16801 acgatttttc cgtccgcgcc gcgcgcgacc gcgtatccgc gccgcagcgt gcccagcggc
    16861 gagagcttct ggagcttttc tgacgcgacc gccaaacgca tttccgtgcg cgcaacctgc
    16921 gctgcggcaa gccgtcgcac atcgggcacg gacaggaacc ggcggcgctt ctccgcgacg
    16981 cgacgctcga gcgcaacgat catgccgcgt tcccgctccc gcacgagcgc catgcgcgtc
    17041 tccacgatgt cgcgcggagt gcggaacacg cgcacggagg tcgcggcgtc gatgcgcagg
    17101 gtcgcctcgc gaacatacga gagcatcgcc gaataggccc gcgcgcggca gtagtcgatg
    17161 cgctcctgca ggtccacctt caccggaacg gcgatctccg cggcggccga cggcgtgggc
    17221 gcagcggcgt cggcggcgtc gtcgcacagg ggatgatcca cctggtggcc cacggcggag
    17281 atgatgggaa cgcgcgaatt cgcgaatgcg cgcacgacga cctcctcgct aaacggcatg
    17341 aggtcctcga aggagccgcc gccccgccct gcgatgatca catcgacccc atgcgacggg
    17401 tcgttcagct cctcgatacc gcgtgcgacg gaatcggccg cgcccgctcc ctgcaccagc
    17461 gccggcgcga tgaggatctc gacgttcggg aatcgccgcg tcgcgacctt gatgatgtcg
    17521 cggagcgccg cccccgtggg cgaggtgacc acgccgatcc tgcgcggcag gaaggggatc
    17581 cgccggcgcc gcgcaggatc gaacagcccc tccgcgagga gttttttctt gagctgctcg
    17641 atgcgcttct gcagctcgcc cgctccctcc aggcgcacct gcgaaacggt gaactggtag
    17701 ctcccgcgct tctcgaagag cccgacgctc ccgaaggcga acacgctcat cccctcctcg
    17761 agccggaagg ccatcgatcg gttggcgtac ttgaagaaca ccgacgatat cacagccccc
    17821 tcgtccttga gcgtgaagta gatgtgcccg gaggaatgat aggtgagatt ggtgatctcg
    17881 cccttgagcc agacctggtt cagctccggg gttccggtga gggtggcctt gatgaggcgg
    17941 gtgaactcgc tgatcgtgta gaaccggtcg ttcatggcgg gtacggcata gcgcaccgat
    18001 ctaatttttc aaccgaaaaa cgagaaggat acctatcgat agccaaatgc cacgccgttg
    18061 agattctgcg gggtcccgga agttgccgga atccagttgt cgccattgtc gagtgaatag
    18121 tacaccgaac ccccggtgcc gaccgccacc catacattgt tgccaaatgc gaccccctgc
    18181 agaacggtcc cgctgaatgt cgtgttgttc cagctcccgg agccggtata gtaataggcc
    18241 atgccggtgc cgccattgtc gcccaccgcg atatatctgc cattcccata gtcgatgtcc
    18301 ttcatgtacg ccggtgcaat aggcataccg gtgcttccga ccgcccatgt tgcgccgttc
    18361 gaagaatgaa aggcgtccgg accgttacca acggcgataa actgggaatt ggcgtatacg
    18421 acggcgcgca tcatcgacgt gacccctgag gcgttcgtcg cacctgtcgt aaaccaatcg
    18481 tcgttatact gggcatagga accgccgctg cacaatagta tccacctatt cagggcggca
    18541 ttataaatga gggaattgac agtactgccc agcgttactg cgggatcata ccatgttctg
    18601 ccgttgtcgt tcgagattct gagatcatta atgccattcc cgaccaccca accccggtcg
    18661 ccgtcactgg cgacagcgct ctcgatcacg agcggcccgc ttccgggaat ccatgtgttc
    18721 ccgccatcct ccgagaaacg gacaaaggag cccattccca ccgcgacaaa cgtttttttc
    18781 ccgttacttg aaaccgcata gagtacatcc gaaccaccgg caccagaggt cccagccgac
    18841 caggttatgc catcaccaga ataaataatg gttccattcg ctccaacggc gacaaagacc
    18901 ggatcgccct tgaattgcgt tgcggactcc gtcttctgag cgtcagggaa gagatcattc
    18961 tgtttgcagg acgcgaaacc agcgccgcat acgaggaaca gaagcactac gccgagacgt
    19021 ttcatcgctc tactccgtat gattctgatg tggtacgcgc gtgatcgcac cccatacgcc
    19081 gagaaaacat gcgtcaagat aatttcacgg cggacaccgc aatcctcctt gctttacatg
    19141 cgccccgttg tgatccccca tcctccacgt cggacctgct cagatgcgca tatcgagtga
    19201 atgccccctc gctaccacgc cactaccacg tctcgatcac gaggatcacc ggatcgaagc
    19261 atcacgcacc aggggtctct gcggctcccc cgaactggat gagatacacg gccccgatcg
    19321 acagcaggcc gcccgcgatc gtggagaccg ccggcacctc gccgagaacg acccagctga
    19381 gcagcaccgc cgtgagcggg accatgaagg tgaaggcgct ggcgcggcgt gatccgatct
    19441 ttttcgcggc gtagaaatag agcgacgtgg cgcccaccgt cgagaagagc gaaaggtatg
    19501 tcatcgaaaa ccaccaggac ggctcggcgg acacgaggcc gtcgaggccg tacgggagca
    19561 cgaacaggaa ccggatcagc gccgcgaagc cgttcaggta gaagctgtac acccaaatag
    19621 aaacggatga ttgcgtccgc tggctgttca cggtgacgcc cgaccagagc gctgcgccga
    19681 tcacgaaaaa gaggtttccc gaggcgagca gcgcttcgtg ggaaaatcgc catatctgca
    19741 ggatgaccag cgcccccgcg cacccgatcg caaggcccgc cgcatcgatc acccgaagct
    19801 ttttccttcc caggacggac acgccggcat aggtgatgat gggcaccagc gaggtcacca
    19861 gcacgccgcc caggccggcg gttcccagcg acaggcccag aaaaaacatc tggctgtaga
    19921 gcgtgtacag gacgctcccg aggaggatca gcccggcggt ggcgggccgt atccgtaacg
    19981 attcacgcag cgcgatgatc ggcggaacaa agaacaaaac ggtgagcgtg aagcgcacga
    20041 aggcgagttt ctgataatgg atgccgttgc cgagcgcctt tgcgcacacc cacgaaccgc
    20101 cccacaccgc catcgcgagg gccatgagtc ccatgaagag agcgtcgcgg ttgccggcgt
    20161 ggaatgttcg catgcggtat gatccggtgc gcctgatcgg cgcgctcaca ggcccggctt
    20221 cacctcgaag gtgacgcgcc ggttggattg atcgcgcgga tcgagccagg ggatcgtccg
    20281 cgacgatccg tagcccttgt ggcgaagcag ggatcccttg atgccgatgc cgaccatcgc
    20341 ctggtagacc tcgcgcgcgc gatcgtccga tatgcggata tttcccggct tgccgtcgcg
    20401 cggctcctcg ggaccggagc aacaggtgtg tccctgcacc tcgatcacgt atccctgcgg
    20461 aagggtgttc gccatcgccc ggaggacgtc ggcattggcc cggacccagg ccgtggggtc
    20521 ccacaccttg ctcgatttat acaggaaccc gtacatgatc atcccgttga ggctgccctg
    20581 cgcccgctcg acgcgcgcgt ccagcgacga acggacgtcg ccgacatcca tggcgcgcgc
    20641 gtagttgtcc cgcgccgcct cgtagcggcc gtcgcgctcg atggatttcg accagtaggc
    20701 catggcgatg ttccggtagc gttccgcctc gacctcgcgg gagcggtagc gcgcctcgag
    20761 cgagcgatag tactgcatct tgccgatata gttgagccga aaggcctcat ggtcgcgcgt
    20821 ctcgccgacg acgagctgct gcttctcctg cgcggtgagc gggatgccga ggcgcttggc
    20881 gatcttgatg gccagaagcc ccctgagttc ggtccaattc gcggggggtc cctcctcgcg
    20941 ctcgcttgcc agcaccgtat tgtcgcggct ccggatcacc ttcgccgtga tcgtcatcgt
    21001 cttccccttg tcggcggtgg acaccgtgcc caggacggcg taatcggtgt cgagcgtccg
    21061 gagcgcgccc agcagctgct gctcttcttt cgcggtgccg tagacctgcg agatggtcag
    21121 ccgatcgaac aggccggcgg gatcgatgga ccggaaaatt cccgaggcct gcatgcgccg
    21181 cgcgagctcg tcggcgacgc ccacggcgag atgatcgttc gcctcgacac cgctctggtt
    21241 ctggaacggc ggtatcatca gcgtccgcac cggacgcgcc gtcttctgcg gggagccgca
    21301 ggcgaggatg atcgcggcaa gcgcaaacgc gcatgtgccg gatgcgatag cgagcctggg
    21361 gacgggaatc atcacacacc tccggtgcgg ttatctgatt atttaatgac cgacacgatg
    21421 gcgaaggtca cccggttgtt cgacttgtcg ccgggatgga tccaggggat gcgcttcaga
    21481 gagccatagc ccttatgggt cattttaaaa tcgaacaccc ctacgccgcg cagggcgtcg
    21541 tacacctgct tcgcccgcgc cgcggccagc gcgggactgc cggccgaatc ggtgtgcccc
    21601 tggatctcca ggcggtattc gtcgggcagc ccgttgatca tcgacttgat gagcacctgg
    21661 ttctttgcgg cccattcggc gatgtgcctg ttcgcgtcgg cctggttcgc gatgccgaag
    21721 atcgtcatct cgttgagccg tccctggatc cgcgcgctct tctcctcctt ggtggagagg
    21781 agcttcttga aatcgaaaag ctgcacatag ttcttccgga cggtctgata gttcacgtcg
    21841 cgggcgatcg ccttgctgta gtgcaggagc gagagctgct tgaacttgcg gacatcgtct
    21901 tctttcttgt tgatccccgc ctcgagcgat ttgtaatagt agagcttgcc gatataattc
    21961 tcgcggaagg cggcgaactc cctggtgtcg gcctccgcga gctgccgaag ggcggtgccg
    22021 ctgagtttca cgcccagctt cgccgcgagg cgcgaggcga gaagcccgcg aaggtgattc
    22081 cactcggacg cctcccccgt ctccgtcgcc tcggccatgg ttttcctgtc ggcggtccgg
    22141 agggcgcggg cggacagggt gagcacgttc cgatcgaccg acaggctcac cgtccccatc
    22201 acggcatact cggccttcag tcccaggaat gcgtccacga gcgccttctc gtccttcgcg
    22261 ccgcccacga gctggcgcag ggtgatcttc tcgaccagga gggccggatc gatcacgtcg
    22321 aagcggtctg aataggccag ccggttggac agttcgtcgg cgatgccgat cgacaggtaa
    22381 tcgtaatcgt cacggttgcc cgcgacggtc gtgtttacaa atggggggat gatgatgctg
    22441 atccgctgct cgtctcccgc ctgcgtaccc gcatcccggc aggaaaacga ggcactggcg
    22501 accgcgacga ccgcacacca gatcaggaat tgcatacgaa ccgatctctt catggcagga
    22561 cccgcattga cgatgggatg ggcgaaacta gccgcccggc cccgattctg tcaacacagt
    22621 aatcgggatg cgcccgcgcc ggttatttga tcaataggaa atacgcgagc accgcgatgc
    22681 ccaggacgat gcggtaccac ccgaagggct tgaagtcgtg gcgggtgatg aagcgcatga
    22741 agatcgcgat caccaggagc gccaccacga acgaaaccgc gaacccgatc ccgagcaccg
    22801 tgagctcgtg cgcacccagc gccttctcgg ccttgagcat cgagtaggcc gacgccgcga
    22861 ccatggtggg gatggcgagg aagaacgaga actccgcggc gaccacgcgc gacgagccca
    22921 ggagcatcgc cccgatgatg gtcgcggccg aacgcgaggt gcccggcacc atcgccaggc
    22981 actgaatgat gccgatgaga aacgccgttt taaacgagag ctcgtccatc gaagtgatcg
    23041 agctctcccg cgcgcgccgc tcgaggacga tgagcacgac gccgcccgcg atgagcgcga
    23101 tggcgaccgt cacgggattg aagagatact ccttgatcgc cttcgcggcg aagaagccga
    23161 ttgcgagcgc cgggaggacg cccacgatgg ttttcagcca tatcccgaag acccgcttcc
    23221 gctcgtcggc ggtgatgccg ccgccaaagg gaatgagccg acgccagaag tatacgacca
    23281 ccgtcaggat cgcgccgagc tgaatgacca catcgaagag
//
LOCUS       DAORHF010000035        20878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1738_length_20878_cov_5.325025, whole genome shotgun sequence.
ACCESSION   DAORHF010000035 DAORHF010000000
VERSION     DAORHF010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20878
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1738_length_20878_cov_5.325025"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="PKJ16_04950"
     CDS             <1..1908
                     /locus_tag="PKJ16_04950"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HNV46370.1"
                     /translation="FCPYVISYAFLCWNKLGGRREIIAPAIDFRFGDDHVIDELYDAF
                     RDHADDRAAVARAFAAGRAAQSRFLRERYEYGQRVMREAAAGGATAIVMIGRPYNLYD
                     GIINLGLPGRFHGRGVTAIPYECLIDPDDNSAPVEHVYWNYGQRILSAAKMVRALPNA
                     YAVYVTNFGCGPDSFILGRFEKLMRGKPYLIIELDEHGSETGYLTRIEAFIDVIAEAR
                     VAPARPASENLDFHYLARQRERTVWIPQMHEVIARLFAAGFRAWNFRSEALPLEDADA
                     FETGLKNVRGSECLPAVATIGAFLKHLRDIDAAPAEHALFMPTADGPCRFGQYCVLHR
                     AVLDANGFAEAGLFSPTSINSYMGMPDALRRYLWDVTLAGDLVYKMICARRPYEMTPG
                     SVDARAEEAMGSMERVIEARGDVRECLGRALESIASVPVAYEARPLVGIVGEIYVRCN
                     PFCNDSLVRTIERCGGEAWLSPVSEWIAYTQYMERHLARLNGAGPLGRLFVGLKARYM
                     LGRLHRFETFAAPYLPERTEPPVEEVLEIGKAHLPIIFEGEAILTLGRTEVFLRDGAA
                     MVINCAPFGCMPGNITKTLFAPVQERHGKPVITLFYDGVSDVNRTVGIYLENLRGGMR
                     VEEIEALEKRA"
     gene            1988..2680
                     /locus_tag="PKJ16_04955"
     CDS             1988..2680
                     /locus_tag="PKJ16_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46371.1"
                     /translation="MKVLSRLFLVLPMVFSLVSCSSVWSRDEWSRLSNKEFEFNFLAF
                     RGYPGSFVNSPAITDNDLAIAFISTVDLESVFTALEAKHGIKINRKRYQDGFVNKGRV
                     FAKSVGTTNSFSYWGPLQASSTLGLESFWSASEEQSKSGTNYIDISFHLREAMYGFKY
                     SMIKLQCDVKLFVNGQVRARYFVEFPRLNVWWKTNMETASPQYKKALLLSAVQVKIVA
                     METLLRMIRSGE"
     gene            complement(2685..3605)
                     /locus_tag="PKJ16_04960"
     CDS             complement(2685..3605)
                     /locus_tag="PKJ16_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV46372.1"
                     /translation="MDRTRTLKIASWTSIAGNLALAALKIVAGAVAGSAAVLADGIDS
                     LSDVAISIITLAASLMIARPPDREHPYGHHRAETIAASILAFIIFFIGGQLVILTVER
                     LLYHEPATLPEPLALYATVISIAGKAALAASQFYWGKRSQSRMLMANGQNMLNDIITS
                     AGVLAGLAIGYYWRIPIIDRIIAIAIGVWIMISAARIFRGLAVELMDGHEQDGPYRAI
                     FDAVREVTGAVNPHRARIRRLGALYVIDLDIEVDGAMSVSEAHGIASAIEECIKERLD
                     NVFDIVIHVEPLGNREADEAYGLSANDVQE"
     gene            3827..4753
                     /locus_tag="PKJ16_04965"
     CDS             3827..4753
                     /locus_tag="PKJ16_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46373.1"
                     /translation="MQTYGTTAIAFIMLLSLPLFSSRTAAAADVSAGATVWYAWWRAA
                     HSQVDTDFGISNDRLQTMSAFMYGPVVSVKFHDDWTLSSRFMIGNFGVKELEGRAYGT
                     NYDTFTTRYRVLKYDSDTTLGYSVSRYVRLFAGVKIMGYQRFLNRRSNINIYPFSVLE
                     NYVYITENAFSTTYGPGGGLGLVAPLADGLYASLNVNGLYTVGESQTRLYSYVPNRPV
                     LLGGSDITRYISGEFSTVADVVMWGGNIDAAVSYYILSVRTTVAIGGRFQILKVLARD
                     RRDAVTPSDGSDRDYQDIFGGITVSVLYYFGG"
     gene            4835..5713
                     /locus_tag="PKJ16_04970"
     CDS             4835..5713
                     /locus_tag="PKJ16_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46374.1"
                     /translation="MNKTVKRKSIFVPLVSALMVVAFSGAALAVDLTVGASVWYAWWQ
                     FATKENYDNVSNDVKDNGFMYGPLVSLKFNETWSLSTRFMTGAFGYGEVTGYDYENDS
                     TFISREKTFKFDSDTIVTYSINRMVRVFGGVKWMGYNWYSKNWYSSIEGYPKYIGYKS
                     YESTVGPGLGVGFSIPLADSFYLMINLNGVYTVGTEELVSTYYFYDPDRRNDSGPHVQ
                     IFDLVSWGGNGDIAFAYYFSSAKTTLSLGFRYQQLKVVGRDRKEYLDTQDPAMERDTQ
                     DVFYGVTLSALYYFDM"
     gene            5810..6862
                     /locus_tag="PKJ16_04975"
     CDS             5810..6862
                     /locus_tag="PKJ16_04975"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV46375.1"
                     /translation="MMRQMRRLPFIVIPFIVLAQLSCGKAKLADFVFTVQSSIGTVKV
                     VSQGVARAASIGEALALGDAVITDKASTADLLYGAKGLVRVYENSNVRIASLQEKMAG
                     DTSLTMQSGKVYVTVSKLVKGSTFRVNTPTLTIAVRGTSFQVAAEPQRMRVVVLNGTV
                     TVNPVVADTPVVEAATMVEKNQALVVDEKAMKTAIEKKQAIPVVTLKAEEVEALRREV
                     KEIKPEVIDKLDVETKKEIKREVLQVTEDADASASREAEDRLQAERDAKLAKDKEEQG
                     KRDAGLKDGQAAAARDAAARQAALEKEKRDRAQEAANAERIRAEQERLAREAAERAKA
                     EKERKEKERAGNVPSL"
     gene            6981..7508
                     /locus_tag="PKJ16_04980"
     CDS             6981..7508
                     /locus_tag="PKJ16_04980"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HNV46376.1"
                     /translation="MPIKSKIRTILHFPKDGIMFRDITTLLLDAGGFRDVINEMVERY
                     KSVEIDLVVGLEARGFILGGAVAYQLGKGFVPIRKKGKLPAEKESHEYELEYGTDIIE
                     IHKDALKKGDRVLVVDDLLATGGTALASAHLIEKVGGKVVEMSFIVDLPDVGGRKRLE
                     GAGYKVFALCEFEGD"
     gene            complement(7516..8846)
                     /locus_tag="PKJ16_04985"
                     /pseudo
     CDS             complement(7516..8846)
                     /locus_tag="PKJ16_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015732695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
     gene            complement(8955..9545)
                     /locus_tag="PKJ16_04990"
     CDS             complement(8955..9545)
                     /locus_tag="PKJ16_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46377.1"
                     /translation="MNKAERRELIIRHATTLFAEKGYYNVSVTDIIDECRIVRSTFYA
                     HFRNKQDIFRLLVDRFSDILQEAILGINISKADRRDDLVGAIREMSLALVDAIERNRD
                     LTTILVTAPLGHDDDFDDKVNEFYAKILGAIKLLLAEGMRDGTIARLDADIISYAILG
                     NIKQIVLQWIIYRDVPDIRTALDDIVRFILFGITRR"
     gene            complement(9635..10612)
                     /locus_tag="PKJ16_04995"
     CDS             complement(9635..10612)
                     /locus_tag="PKJ16_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV46378.1"
                     /translation="MKKKILMTGGSGFIASHFHERLTDTPVNNLDLVAPAHPLHSAHI
                     QGDVRNPADVERAITGCSSIIHLAAAHHDFGISREEYFDVNENGARVICEAAAKHKVR
                     DVVFYSSVAVFGEDYEMTVDDTEPRPTNDYGASKLAAERVLREWAREDTKRRLLIMRP
                     VVVYGPRNYANMYNLLRQIASGKYFNIGAGGNIKSIAYVENLVEATLFLAARMKAGVE
                     TYSYSDEPHLSSREISDIIARALGRGRTITVPYRLAWLMALPFDALIALTGKNLPVST
                     NRVKKFCTQTYHDAPKVRASGFTKRFDNPAGLARMARWYLDTPSARERP"
     gene            complement(10658..11944)
                     /locus_tag="PKJ16_05000"
     CDS             complement(10658..11944)
                     /locus_tag="PKJ16_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV46379.1"
                     /translation="MTRVVLICSFYAPELGAAVNRVREYSRAFMREPSVDYRVITSFP
                     TYPTGVKPHRYRRKIQTSETGEFGEPVTRLYTTNWSKAFVSKLGNQLMFAFMLGQYCL
                     RFLFLRNTVFIVSSPSFFAIIPVYLLSLVRRCDYVLDVRDPYPVSLFELSGLVKRDSL
                     PGRILLALEARFYRRARRVFTVSESIIDFIMASNPRIAARGIGRERFGVIYNGVSADF
                     IGFGADEAHRREIAARYRGRFVVLFSGTMGKAEDIEAVLEAAAILEHTHPKMLFLFVG
                     DGEKRGMVAAHAARLRNVALVDPVSHRAVAAYIRVANVGLSIRHPSPLEATALPVKIF
                     EYLAMGLPVIYGGGGEAERVIAGARGGIALETTSGAALAAALRTVHARKTTLRVNREY
                     IIRNFSRETQAKKMVAEIVSPHSPPCARDNAAGTRA"
     gene            complement(11941..13038)
                     /locus_tag="PKJ16_05005"
     CDS             complement(11941..13038)
                     /locus_tag="PKJ16_05005"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV46380.1"
                     /translation="MIKFLDLAKQYQSIKGEIDAAIGAVIADTSFIGGAHLRRFEEAF
                     AAYQRAEHCVGVANGTDAIEIAIEALGLPHGSEIIVPANSFIASSEAVTRAGCRVVFC
                     DANERNYTIDIDDLSRRITPQTKAIIAVHLYGHPCDMDALLAIAKRHGLFVIEDCAQA
                     HGAEYRGRRVGAIGDIGAFSFYPGKNLGAYGDAGAIVTNDAALARRCRMIANHGRVEK
                     YNHEFEGRNSRLDGLQAAVLSVKLAHLDEWTARRIAIADYYLEHLRDVPFITLPERAE
                     WARQVYHLFVIRSEHRDALQAHLKAGGVETGIHYPIALPKLAAYRYLNQHGEALFANC
                     ADSTLLSIPMGEHLEPEDARTVVERIKAFSP"
     gene            complement(13090..13683)
                     /locus_tag="PKJ16_05010"
     CDS             complement(13090..13683)
                     /locus_tag="PKJ16_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HNV46381.1"
                     /translation="MIAPSAIIHPGVRLGADVTVEDFCIIGAPFRGQAGEETVIGDGA
                     VIRSHTVIYAGNVIGAGFQTGNKANIRELNRIGDDVSVGTLTVIEHHVTIGSGVRIHS
                     QVFIPEFTELMDECWIGPNVVITNARYPKSPEAKQNLRGAVIEPNAKIGANCTLLPGV
                     RVGAGSLVGAGSVVTKDVAPGIIAAGNPARRIKNIFY"
     gene            complement(13680..14738)
                     /locus_tag="PKJ16_05015"
     CDS             complement(13680..14738)
                     /locus_tag="PKJ16_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007420597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV46382.1"
                     /translation="MLRFALVGCGRIAKRHSELLGFGQIAGAELAAVCDIVPEKAKAI
                     GERFSVPHYTDMDEMMTREKIDAVTVLTESGRHAEHVIRLAKHGRHIIVEKPMALTLD
                     DADRMIAACAENDIRLFVVKQNRFNVPVQKLRGALEAGRFGTLVLGTVRVRWCRTQAY
                     YDQDAWRGTWAFDGGVLTNQASHHVDLLEWMMGDVESVFAKGITALVNVETEDTAVVT
                     LKFKNGALGVIEATTAARPKDLEGSISILGERGTVEIGGFAVNEMKVWNFTEPIAGDD
                     EVLSKFSVNPPNVYGFGHQAYYEHVVDSISNNTKQLVDGLEGRRSLELISAMYESMET
                     GREVLLRFSPNKCRLGMR"
     gene            complement(14731..16017)
                     /locus_tag="PKJ16_05020"
     CDS             complement(14731..16017)
                     /locus_tag="PKJ16_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV46383.1"
                     /translation="MVRLITDIGDGPRRTRCEYAFRTLLDVAGVSPDNALAVSYASEP
                     HTRCDVWIRPEGAPAIARRRRGAIAIDDDIIAESFALLSCAEEYGGRRDGKGRFLARF
                     TERAAIDIPVVNRHALTLKRALARAARAKGKRLRTDPRRFTVVLSHDVDNITDKNIHV
                     CLHRAARAVKYLLRGRIASALRMGLFTLGRLFARGNPYDNYAAYIDLERRYGFRSTFF
                     MITGAGGRFGARYSIAQARSMLGDITAAGWEVGLHTNYYSFHDPKAVIGERRAIEDAI
                     GRPIAGCRNHYLMLSIPESWRVMVRAGLVYDSSVGYTDAIGFRAGVAYPYRPYDMAAE
                     AAFDLIEIPLVIMDTAVLDYDGDREAAWARIQKVLDETRDVGGTIAVNFHHRALYDRE
                     FAGWNDIYIRILDYIREHNGRGITASMLVGEVVHHA"
     gene            complement(16030..17034)
                     /locus_tag="PKJ16_05025"
     CDS             complement(16030..17034)
                     /locus_tag="PKJ16_05025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV46384.1"
                     /translation="MARTVKTRILPQEEYERWDAFVDTSPQGSLFNKSWWLSTVSSDF
                     SILACEDGSGRIIGGIALPRYYGTLYKNPPITTVLGVLLGDFSDLKNATRYSKEIEIV
                     EEIVASLPRFRLFDYGFSYHFTNALPFKWAGFSFDIGCTYVIEDLSAVDALFDDFKTN
                     IKTDVRKAAKNGVSITDALTIDDFYAVHRMTFQRQSMEVSYGKDFVLGIERTLAARNA
                     RKMLFAVDEAKRIHAAAYIVHDARCAYYFMGGGDPALRNSGATSLVIWEAIRFAATVS
                     REFNFEGSSVPSIERFFRAFGGEQRPYHIIRKSSAVTRMALSLLRTQRNLIYRFRKLP
                     "
     gene            complement(17137..18099)
                     /locus_tag="PKJ16_05030"
     CDS             complement(17137..18099)
                     /locus_tag="PKJ16_05030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV46385.1"
                     /translation="MARPPFSTTRLLERSRSAGMDDKTPSAAMRGGLRMNGFVKRSRP
                     GLPLVTVATVSYNAAADIEDTIRSVLEQTYDNVEYLVIDGGSTDGTVDIIRAHEHAID
                     LWICEPDRGIYDAMNKGIALGTGDWFSFMNAGDRFAGDDVLALFCDARFDAHAPGIAY
                     GDTIVVDAQSREILSSAGELRLRDFHYDTPVCHQSLFTNRMAFEAVGAYSADLKLMAD
                     LDWLVRFMRLGATRTCNRYRVDRPVARYRIGGKGYSSGERNYLERWRVARAYFPLRVR
                     ARYLIGFPLEMVLIRLRRLAKLILGDSAIAYRWRRWRMGGSLSP"
     gene            complement(18115..19215)
                     /locus_tag="PKJ16_05035"
     CDS             complement(18115..19215)
                     /locus_tag="PKJ16_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HNV46386.1"
                     /translation="MNKDSRIFVAGGSGLVGAAIIRTLLQKGYTNVIASSHTSRVDNA
                     GDRRVPEYPLDLTDQAATNEFFRTHTPEYVFMSAARVGGIAANSTYVAEFIYQNIAIA
                     ANVVHAAHTHGVTKLLNLGSSCIYPKLAPQPMKEDYLLTGPLEETNEPYAIAKISAIK
                     LCRYYNQQYGTNFISVMPTNLYGPNDNYDLYNSHVLPALIRKFHLGTLLEKNDLAAIR
                     ADFNFWERTDRYGAMSDADIMRLIETFGITKGRVAVWGTGSPFREFLHVDDVTDAFVF
                     LMENFDYADIGEFINIGYGADVTIREAAGTVKDIVGFTGELYFDTTKPDGTPKKLLDV
                     SRLSALGWKAKIPLRDGIANAYEWYVQTVPSR"
     gene            complement(19208..20368)
                     /gene="gmd"
                     /locus_tag="PKJ16_05040"
     CDS             complement(19208..20368)
                     /gene="gmd"
                     /locus_tag="PKJ16_05040"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV46387.1"
                     /translation="MKKKALITGITGQDGAYLAELLMNKGYEIHGIKRRSSMFNTHRI
                     DHLYQDQHESNVKFFLHYGDLTDSTNLIRIVQDVQPDEIYNLAAQSHVAVSFETPEYT
                     ANADAIGTLRLLEAIRILKLENKTKFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYSYWIVVNYREAYDIFACNGILFNHESPNRGETFVTRKITRAAARIALGLQNMMFL
                     GNMNSKRDWGHARDYVEGMWLMLQQKTPGDFVLATGETNTVRTFVEMSFHELGVSVEW
                     KGEGVHEKGKIAAIDKDAFMKAIDDAALAEKALKSLKTGAIVVEVDPRYFRPTEVDLL
                     IGDPSKAKRELGWAPKRSLRDLVGEMVREDVREARKGVLLHESGYRVMRFHE"
     gene            20518..>20878
                     /locus_tag="PKJ16_05045"
     CDS             20518..>20878
                     /locus_tag="PKJ16_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HNV46388.1"
                     /translation="MIDHGALALPPERVIILDFGSQYTQLIARRVRELGVYAEIVPYF
                     HPLDDITRERPSAIILSGGPSSLYEDGAPRITADIFDLGVPVLGICYGLYVIVDAFKG
                     TSQPAHSKEYGRATITIA"
BASE COUNT         3810 a         6251 c         6823 g         3994 t
ORIGIN      
        1 ttctgtccct atgtgatctc gtatgcgttc ctgtgctgga acaagttagg cggccggcgg
       61 gagatcatcg cgccggcgat cgatttccgc ttcggcgacg atcacgtgat cgacgagctc
      121 tacgacgcct ttcgggacca cgcggacgat cgcgccgcgg ttgcgcgggc cttcgccgcg
      181 ggccgcgcgg cgcagtcgcg ctttcttcgc gagcgctacg agtacggaca gcgcgttatg
      241 cgcgaggcgg cggcgggcgg cgcgaccgcg atcgtcatga tcggccgtcc gtacaatctg
      301 tacgacggca tcatcaacct tggcctgccc gggcgctttc acggccgcgg cgtcacggcg
      361 atcccctacg agtgcctcat cgaccctgac gacaacagcg cgcccgtgga gcacgtctac
      421 tggaactacg ggcagcgcat tctctcggcc gcaaagatgg tgcgcgcgct cccgaacgcc
      481 tatgcggtgt acgtgaccaa cttcgggtgc ggcccggact cgttcatcct cgggcgcttc
      541 gagaagctca tgcgcggcaa gccgtacctc atcatcgagc tcgacgagca cggctccgag
      601 acgggctatc tcacgcgcat cgaggcattc atagacgtga tcgcggaggc gcgcgtcgcg
      661 cccgcccgcc cggcgtcgga gaatctcgac ttccactatc tcgcgcgcca gcgtgaacgc
      721 acggtgtgga tcccgcagat gcacgaggtg atcgcccgtc tcttcgccgc gggttttcgc
      781 gcctggaact tccgctccga agcgcttccg ctcgaggacg cggacgcctt cgagacgggc
      841 ctcaagaacg tgcgcgggag cgagtgcctg ccggcggtcg ctacgatcgg cgcgtttctc
      901 aagcacctgc gggacatcga cgcggcgccc gccgagcatg cgctctttat gcccacggcc
      961 gacgggccct gtcgcttcgg gcagtactgc gtgcttcatc gcgcggtgct ggacgcgaac
     1021 ggtttcgccg aggcggggct cttctcgccg acctccatca actcctatat gggcatgccc
     1081 gatgcgcttc gccgctacct ctgggacgtg acccttgccg gtgatcttgt gtacaagatg
     1141 atctgcgcac ggcgcccgta cgagatgacg ccgggaagcg tggacgcgcg cgccgaagag
     1201 gcgatgggat cgatggagcg cgtgatcgag gcgcggggcg acgtgcggga gtgcctcggg
     1261 cgcgcgcttg agtcgatcgc gtcggtcccg gtcgcctacg aggcgcgtcc cctcgtcggg
     1321 atcgtcggag agatctacgt gcgctgcaat ccgttttgca acgattcgtt ggtgcgcaca
     1381 atcgagcgct gcggcggcga ggcgtggctc tcgccggtgt cggaatggat cgcctatacg
     1441 cagtacatgg agcgccacct tgcgcgcttg aacggcgccg gtccgctggg ccggctcttc
     1501 gtggggctca aggcgcgcta catgctgggg cggctgcatc gcttcgagac attcgcggcg
     1561 ccgtacctgc cggagcggac ggagccgccg gtggaagagg tgctcgagat cgggaaggcg
     1621 cacctgccga tcatcttcga gggcgaggcg atcctcacgc tgggccgcac cgaggtgttc
     1681 ctgcgcgacg gggcggccat ggtgataaac tgcgcgccgt tcgggtgcat gccgggcaat
     1741 atcacgaaga ccctgtttgc gccggtgcag gaacggcacg ggaaaccggt catcacgctc
     1801 ttctacgacg gcgtgagcga cgtgaaccgc acggtgggca tctatctgga aaacctgcgc
     1861 ggcgggatgc gcgtcgaaga gatcgaggcg ctggagaaac gcgcgtaaaa ccgtatttct
     1921 tcattgaact tcttgacagc gtaacctctc gtgcttttca gtataaaaaa acacgtcgag
     1981 gttcctcatg aaagtactat ctcgcctttt tcttgttctt ccaatggttt tttcactcgt
     2041 ttcatgctct tcggtatggt cgcgtgatga gtggtcacga ttaagcaaca aggagttcga
     2101 attcaatttt ttggcgttta gagggtatcc gggatccttt gtaaactctc cggccattac
     2161 ggataatgac ctggcgattg cgtttatctc gaccgttgac cttgaaagcg tctttaccgc
     2221 cttggaggcg aaacatggta ttaaaatcaa tagaaaaaga tatcaggatg gttttgtcaa
     2281 caaggggcgg gtattcgcaa aatcggtagg aacgacgaat tcgttttcat actggggccc
     2341 gctgcaggcg tcgtcgacgt tggggctgga gtcgttctgg tcggcatcgg aggagcaatc
     2401 gaaatcggga acgaactata tcgatatcag ctttcacctt cgggaagcca tgtatggatt
     2461 taaatactcc atgatcaagt tgcaatgtga cgtgaagcta tttgtcaatg gccaagtgcg
     2521 ggcgcgctat ttcgtcgagt ttccgcgcct caatgtttgg tggaaaacaa acatggaaac
     2581 ggcaagtccg cagtacaaga aggcgttatt gctgtccgcg gtccaagtga aaatcgtggc
     2641 gatggagacc ctcctccgaa tgatccggag cggagagtag cgcgtcactc ctgcacgtcg
     2701 ttcgcgctga gcccgtaggc ctcgtccgcc tcgcggttcc cgagcggctc gacgtgtatc
     2761 acgatgtcga agacattgtc gagccgctcc ttgatgcatt cctcgatcgc gctcgcgatg
     2821 ccgtgcgcct cgctcacgct catcgcgccg tccacctcga tgtccaggtc gatgacgtat
     2881 aacgcgccaa ggcgccggat ccgcgcgcgg tgcgggttga ccgcgccggt cacctcgcgg
     2941 acggcatcga agatcgcccg gtacgggccg tcctgttcgt gtccgtccat gagctccacc
     3001 gcgagccccc ggaagatccg cgccgcgcta atcatgatcc acacgccgat cgcgatcgcg
     3061 atgatgcggt cgatgatcgg gatccgccag tagtacccaa tcgcgaggcc cgcgagcacc
     3121 cccgccgatg tgatgatgtc attgagcatg ttctgcccgt tcgccatgag catgcggctc
     3181 tgcgaccgct tcccccagta gaactggctg gcggcaagcg ctgcttttcc ggcgatggaa
     3241 atgacggtcg catacagcgc cagcggctcg ggcagagtcg cgggctcgtg atagagcagg
     3301 cgctccacgg tgaggatcac gagctgtccg ccgatgaaaa agatgatgaa cgccaagatc
     3361 gacgccgcga tggtctcggc gcggtggtgc ccgtacgggt gctcgcggtc cggcggtcgc
     3421 gcgatcatga gcgaggcggc gagggtgatg atggagatgg ccacgtcgga caggctgtcg
     3481 atgccgtcgg ccaggaccgc cgcgctcccg gctacggctc ccgccacgat cttgagcgcg
     3541 gcgagcgcaa ggttgcccgc gatgctcgtc caggaggcga tctttagtgt gcgggttctg
     3601 tccatggtca aggcgtattc cgttcgaaca ggtttgatcc tggcgcatgt ctgcggattt
     3661 gtcaatcacg aagccgaggc catttcgcat ccgaaatgcg gcatgcgggc gacgatccgg
     3721 attttaccgc ttcacatgga aaagggcttg aatttctatt cggcggttgg ttccattgac
     3781 cctcgagagc atgacagtat gtcaccaccc ccatttggag gcgatgatgc aaacgtacgg
     3841 taccaccgca atcgctttca tcatgctctt gtctcttccg ctattcagtt cccgcaccgc
     3901 cgctgccgcc gatgtctccg ccggcgcaac cgtctggtac gcgtggtggc gcgccgccca
     3961 ttcccaggtc gataccgatt tcggaatcag caatgaccgc ctgcaaacga tgagcgcctt
     4021 catgtacggg cccgttgtct cggtcaagtt ccacgacgac tggacgctct cgtcgcgttt
     4081 catgatcggg aatttcgggg tgaaggaact ggagggacgg gcctacggaa cgaactacga
     4141 tacgtttaca acgcggtatc gggtgctcaa gtacgattcg gatacgacgc tcggatactc
     4201 cgtttccaga tatgtgcgtc ttttcgccgg cgtgaagatc atggggtatc agcggttcct
     4261 caatcggaga agcaatatca acatctatcc gttttccgtt ttagaaaact atgtgtacat
     4321 aaccgagaat gctttttcca ccacctatgg gcccggcggc ggtctggggt tggttgcccc
     4381 gcttgccgat gggttgtatg cgagcctgaa cgtcaacggc ctgtataccg ttggggaatc
     4441 ccagacgcga ttgtacagct atgtgcccaa caggccggtg cttcttggcg ggagcgatat
     4501 cacgcgctat atcagcggag agttctcaac cgtggccgat gtcgtcatgt ggggtggcaa
     4561 catcgatgcg gcggtttcgt attatattct gtcggtgcgg accaccgtcg cgattggcgg
     4621 acgcttccaa atcctcaagg tgcttgcccg cgaccgccgc gatgcggtta ctcccagcga
     4681 tgggagcgat cgcgactatc aggacatatt cggcgggata acggtgtcgg tgttgtatta
     4741 cttcggcgga tgatgcgtgc ggcgtgatct cgtcagtacc gccggcgtat cgggttgtga
     4801 tgatagtgct gataactcga agaggagaca tgacatgaat aaaaccgtaa aacgaaaatc
     4861 gattttcgtg ccgctcgttt ccgcgctcat ggttgtcgcg ttttccggcg ccgcgctcgc
     4921 cgtcgacctc accgtcggcg catcggtctg gtacgcctgg tggcagttcg cgaccaaaga
     4981 aaattatgat aacgtcagta acgacgtcaa ggataacggc ttcatgtatg ggcccttggt
     5041 ttcgctgaaa ttcaacgaga cgtggtcgct ctcgactcgg tttatgactg gggccttcgg
     5101 ttatggggag gtaaccgggt acgactatga aaatgacagc acgtttatca gtagagaaaa
     5161 aacattcaag ttcgattccg acaccatcgt gacgtattcc atcaacagaa tggtgcgagt
     5221 tttcggcggc gtcaaatgga tggggtataa ttggtattca aagaactggt attcatcgat
     5281 cgaggggtac ccgaaatata ttggttataa atcatatgaa agcaccgtcg gtccggggct
     5341 tggtgtcgga tttagcattc cgcttgccga cagtttctat ctcatgataa acctgaatgg
     5401 cgtctacacg gtcggaacgg aggagttggt gtcaacatat tacttctacg accccgaccg
     5461 aagaaatgat tccggcccgc atgttcagat atttgacctc gtttcctggg gcggcaacgg
     5521 tgacatcgcc tttgcatatt atttctcttc cgcgaaaacg accctttcgt tgggtttccg
     5581 ctatcagcaa ctcaaggtgg ttggacgcga tcggaaggag tatttggata cacaggaccc
     5641 cgccatggaa cgggacacgc aggacgtgtt ctacggtgtc acgctatcag cgctgtatta
     5701 cttcgacatg tagcatgcga aacagtgccg cgaacggcaa tcaccagctc ggaaccatgc
     5761 ggacgggttc gttgacgcgc cacaagaaca atacacccga gaggtattca tgatgcgaca
     5821 gatgcgacgg cttccattca ttgtcattcc gtttattgtg ctcgcccagc tctcctgcgg
     5881 caaggccaag ctcgcggact tcgtctttac ggtgcagtca tctatcggta cggtgaaggt
     5941 cgtgtcgcag ggcgtggcgc gcgccgcgag catcggcgag gcgctcgcgc ttggtgatgc
     6001 cgtcatcacc gacaaggcct ccaccgccga cctcctctac ggcgcaaagg ggctggtgcg
     6061 cgtgtacgaa aactcgaacg tgcggatcgc ctccctgcag gaaaagatgg cgggcgatac
     6121 cagcctcacc atgcaaagcg gcaaggtcta cgtgaccgtc tccaagctcg tcaagggctc
     6181 gaccttccgc gtgaatacgc cgacattgac catcgcggtg cgggggacga gcttccaggt
     6241 ggccgccgag ccgcagcgga tgcgcgtggt ggtcctgaac ggaacggtca cggtcaatcc
     6301 cgtggttgcg gacacgccgg tcgtggaggc cgcgaccatg gtggagaaaa accaggccct
     6361 ggtcgtggac gaaaaggcca tgaaaacggc gatcgagaag aagcaggcga tacccgtggt
     6421 gaccctgaag gccgaggaag tcgaggccct ccggcgggag gtcaaagaga tcaagcccga
     6481 ggtgatcgac aagctcgatg ttgaaacgaa gaaagagatc aagcgcgagg tgttgcaggt
     6541 cacggaggac gccgacgctt cggcgtcccg cgaggcggag gaccggctcc aagcggagcg
     6601 tgatgcgaag ctcgcgaaag ataaggaaga gcagggaaag cgggacgccg ggctgaagga
     6661 cggtcaggcg gccgctgctc gcgatgcggc ggcgcgtcag gccgcgctgg aaaaagaaaa
     6721 acgagatcgc gcgcaggagg cggcgaacgc ggagcgcatt agggccgagc aggagcgact
     6781 cgcgcgcgag gccgccgagc gggcgaaggc cgagaaggaa cggaaggaaa aggagcgcgc
     6841 gggcaacgtg cccagcctgt aggaacggcc gtcgtgtgcc gggataaaag tatttgcttt
     6901 ttcaatgccg tgacgatgtg atgtgtcggc gggcgcaggt cgctcgccgg tgatttgcat
     6961 acaaccctga aggtgccatc atgcccatta aatcaaaaat tcgcaccatc ctgcacttcc
     7021 ccaaggacgg gatcatgttc cgcgacatca ccacgctgct cctcgacgcg ggcgggttcc
     7081 gcgacgtcat caacgaaatg gtggagcgct acaagagcgt cgagatcgat cttgtggtcg
     7141 gtctcgaggc gcgcggcttc attctggggg gagcggtggc ctatcagctc ggcaagggct
     7201 tcgtccccat ccgcaagaag ggcaagctcc cggccgagaa ggagtctcac gagtacgagc
     7261 tggaatacgg gaccgatatc atcgaaatcc acaaggacgc cctgaaaaaa ggagaccgcg
     7321 tgctcgtcgt ggacgatctt ctggccaccg gcgggactgc gcttgcgagc gcgcatctca
     7381 tcgaaaaggt cggcgggaag gttgtcgaga tgtccttcat cgtggacctg ccggacgtgg
     7441 gcggtcggaa gcgcctggag ggggcgggct acaaggtctt tgcgctctgc gagttcgagg
     7501 gcgattgagc gcgcctcaca ccttcaggaa cgcatagcgc agcacaaaca cgatccccag
     7561 cacgatgacc agcggcttga tctcgcggat cttgcccgtc gcgatcttga gcgccgggta
     7621 ggagatgaac ccgaaggcga ggccgtcggc gatcgagtag gtgagcggca tcccgatcat
     7681 aaccaggaag gccggcaggt attcggtcac gtcgtcgaag gcgatgtccc tgatgctaat
     7741 gaccatcatg gagccgacaa tgatgagcgc cggcgcgatg atcgggtaga acgtcgtccc
     7801 gccgaccgcg tatccgccgc cgatcatccg cgcgagcggc tcaatgaaga tggcgagcgc
     7861 caggagcagc gccgtgacga ccgcggtgag gcccgtccgc ccgccctcgt acacgcccgt
     7921 cgcgctctcg atataggtgg tcacggtgga ggtgccgaag agcgccccga aggccgtcgc
     7981 ggccgaatcg gtgtgcagga ttcggttgat gcgcgggatg gcgccgttct cgtccatgag
     8041 ccccgcctgt cgcgtaacgc caacgacggt gcccaccgta tcgaacacgt tcataaagag
     8101 gaacaccagg atcaggggga catagtcgag cgagagcgcc ttgaggacgt ccatcttcgc
     8161 gaaaactggc atgatggacg gcgggccgct cacgatgccg cggtagttcg cgatcccggt
     8221 cacgagggcg atgagcgcgc agacgaggat tcccaggagg atcgcccccc ggatcctgaa
     8281 atacgacagg acggcgatga ccagcacccc gagtatcggg atgatcacgt gcagctcgcg
     8341 gacgctgccg agcttcacga tcccgcccgg cgatttctcg atgatgcccg cgaagaccag
     8401 gccgatgaac gtgacgaaca ggccgatgcc caccgtgatg ccggccttga gcgacggcgg
     8461 aatcgtgttg atgatgatct cccgcacatt gaacagcgac agcgccagga acaggatgcc
     8521 ggagatgaac acgatgccca gcgccgtgtc ccacgggatc cccatcgcga cgaccaccac
     8581 gtatgcgaag aagaaattct cgcccatgcc cggcgcaagc gcgagcggat agcgggcgta
     8641 gaggcccatc atgagcgtgg cgagcgccga ggagacgcag gtcgctacga gcaccgcgcc
     8701 gaagtccatc ccggcggcgg agagcacggc cggttgcacg aagatgatgt aggccatcgt
     8761 gacgaaggtg gtggcccggc ggtgatctcg gtgcgaatcg ttgacccgag ccgggagatt
     8821 gagaagaaac ggtcgatgaa gttcatgccg gcaccccgca tgcggtattg tcgatcgggc
     8881 ggcggccccg ccccggtcga cggtccgatt tgatcgcgcg gcggaaaatt atcaaatgaa
     8941 atttgcgaaa aaaatcaccg gcgggtgatg ccgaacagga tgaagcggac gatgtcgtcc
     9001 agcgccgttc ggatgtccgg aacgtccctg tagatgatcc actggagcac gatctgtttg
     9061 atgttgccca ggatcgcgta ggagatgatg tccgcgtcga gccgcgcgat ggtgccgtcg
     9121 cgcatgccct cagccaggag gagcttgatg gcgcccagga tcttcgcgta gaattcgttc
     9181 accttgtcgt cgaaatcgtc gtcgtgcccg agcggcgcgg tcaccaggat cgtcgtgagg
     9241 tcgcggttgc gctcgatcgc gtccaccagc gcgagcgaca tctcgcggat cgcgcccacc
     9301 aggtcgtccc ggcggtccgc cttggagatg ttgatgccca ggatcgcctc ctggagaata
     9361 tcggaaaagc gatccaccag taggcggaag atgtcctgtt tgttccggaa atgcgcgtag
     9421 aaggtgcttc gtacgatgcg gcattcgtcg atgatgtcgg tcacggagac gttgtagtag
     9481 cccttctccg cgaagagcgt cgtggcgtgc cggatgatga gctcgcgccg ctcggccttg
     9541 ttcatggcgt ccgtcccgcg ccgcgcgcct ctccatcgcg cgcggcgtcg gtgaagggaa
     9601 gcgcgttctg tgcgatgcca aacatcggcg aactctaggg ccgctcccgc gcggacggcg
     9661 tatcgagata ccaccgcgcc atccgcgcga gtcccgccgg attgtcgaac cgcttggtga
     9721 agccgctcgc gcggactttg ggggcgtcgt gataggtctg cgtgcagaat ttcttcacgc
     9781 ggttggtcga gaccggaagg ttttttcccg tgagggcgat gagcgcgtcg aacggaagcg
     9841 ccatcagcca cgcaagccgg tagggaacgg tgatcgtcct gccgcgcccg agcgcgcgcg
     9901 cgatgatgtc ggagatctcg cggctcgaga ggtgcggctc gtccgaatag ctgtaggtct
     9961 cgacgcccgc cttcatccgt gcggcgagga agagggtcgc ctcgaccagg ttttccacgt
    10021 aggcgatgga cttgatgttg ccgccggcgc cgatgttgaa gtatttcccc gaggcgatct
    10081 gtcggaggag gttgtacatg ttcgcgtagt tgcgggggcc gtataccacg acgggacgca
    10141 tgatgagcaa tcgacgtttt gtgtcctccc gcgcccattc gcgcagcacc cgctcggcgg
    10201 cgagcttgga ggcgccgtaa tcgttcgtcg ggcgcggttc ggtgtcgtcc accgtcattt
    10261 cgtagtcctc gccgaagacg gccacgctgg agtagaagac aacgtccctg accttgtgtt
    10321 tcgccgccgc ctcgcagatg acgcgcgcgc cgttctcgtt cacgtcgaag tactcctcgc
    10381 gcgatatccc gaagtcgtgg tgcgccgcgg ctagatggat gatggacgag cagcccgtga
    10441 tcgcgcgctc gacgtccgcg gggttgcgca cgtcgccctg gatgtgcgcg ctgtgcaggg
    10501 ggtgcgccgg cgcgacgagg tccaggttgt tgacgggcgt gtccgtgagg cgctcgtgga
    10561 aatgcgatgc gataaatccg gaaccgccgg tcatcagaat cttctttttc atgtcccgtg
    10621 tccttgatgg aattctttca ctgcccccag aggggcgtta cgcgcgtgtt cccgccgcat
    10681 tgtctcgagc gcacggagga gagtgcgggg atacgatctc cgccaccatt tttttcgcct
    10741 gcgtctcacg cgagaagttc cggatgatgt attcgcggtt gacgcgcagc gttgtttttc
    10801 tcgcatgcac ggtgcgaagc gcggctgcga gcgcggcgcc gcttgtcgtc tcgagcgcga
    10861 tgccgccccg ggcgccggcg atgacccgtt cggcctcgcc gccgccgccg tagatcaccg
    10921 gaagccccat cgccaggtat tcgaatatct tcaccgggag cgcggtcgcc tcgagcggcg
    10981 agggatggcg gatggacagg ccgacgttcg cgacccggat gtacgcggcc accgcacggt
    11041 gggacaccgg atcgacgagc gcgacattgc gcagccgcgc cgcgtgcgcg gccaccatcc
    11101 cgcgcttttc gccgtcaccg acgaacagga agagcatctt cggatgggtg tgctccagga
    11161 tcgccgcggc ctccagcacc gcctcgatgt cttccgcctt gcccatggtt ccgctgaaca
    11221 ggacgacgaa cctcccgcgg tagcgcgcgg cgatctcccg ccggtgcgcc tcgtcagcgc
    11281 caaatccgat gaagtccgcc gagaccccgt tgtagatgac gccgaagcgc tcacgcccga
    11341 tgccgcgcgc cgcgatgcga ggattggacg ccatgatgaa gtcgatgatg ctctcgctca
    11401 cggtgaacac ccggcgcgcc cggcggtaga aacgagcctc gagcgcgagc aggatcctgc
    11461 ccggaagcga atcgcgcttc accaggccgc ttagctcgaa gaggctcacc ggatagggat
    11521 cgcggacgtc gaggacgtag tcgcagcgcc gcacgagcga gaggagatag acggggatga
    11581 tggcgaaaaa tgacggtgag gaaacgatga acacggtgtt gcgcaggaac aggaagcgca
    11641 ggcagtactg acccagcatg aaggcgaaca tgagctggtt tccgagcttc gacacgaagg
    11701 ccttcgacca gttcgtcgta tacagcctcg tcacgggctc gccgaactcg cccgtctccg
    11761 aggtttgaat tttgcgtcga tagcggtgcg gcttgacgcc ggtcgggtag gtgggaaagg
    11821 aggtgatcac gcgatagtcg accgagggct cccgcatgaa ggcgcgggaa tattcgcgca
    11881 cgcggttcac cgccgccccg agctccggcg cgtagaacga gcagatgagc acgacgcggg
    11941 tcatgggctg aaggccttga tgcgctcgac gaccgtccgc gcgtcctccg gttccaggtg
    12001 ctcgcccatg gggatgctca ggagcgtgga gtccgcgcaa ttggcgaaca acgcttcgcc
    12061 gtgctggttc agatagcggt acgcggcgag cttcggcagg gcgatgggat agtggatccc
    12121 cgtttcgacg ccgcccgcct tcaggtgcgc ctggagcgcg tcgcggtgct cggagcggat
    12181 cacgaacagg tggtacacct gccgcgccca ctccgcgcgc tccggcagcg tgatgaacgg
    12241 aacatcgcgc aggtgctcga ggtagtagtc ggcgatcgcg atgcggcgcg cggtccactc
    12301 gtcgagatgg gcgagcttca cggacaggac ggccgcctgc aggccgtcga ggcgggagtt
    12361 gcggccctcg aactcatggt tatacttctc gacgcggcca tgattggcga tcatacggca
    12421 gcggcgggcg agcgccgcat cgttggtcac gatcgcgccg gcgtcgccgt aggcgccgag
    12481 gttcttgccc gggtagaaac tgaacgcgcc gatgtcgccg atcgcgccca cgcgccgccc
    12541 ccggtattcc gcgccgtgcg cttgcgcgca atcctcgatc acgaagaggc cgtgccgttt
    12601 cgcgatcgcg aggagagcgt ccatgtcgca gggatggcca tagagatgca cggcgatgat
    12661 cgccttcgtc tgtggggtga tccgccgcga aaggtcgtcg atatcgatgg tgtagttccg
    12721 ctcgttcgcg tcgcagaaga cgacgcggca cccggcgcgc gtgaccgcct cgctggaggc
    12781 gataaagctg ttcgcgggga cgatgatctc gctcccgtgc gggaggccca gcgcctcgat
    12841 cgcgatctcg atggcgtcgg tcccgttggc gacgcccacg cagtgttccg cccgctggta
    12901 ggcggcaaag gcctcctcga agcgccgcag gtgcgcgccg ccgataaacg aggtgtcggc
    12961 gatgaccgcg ccgatcgcgg cgtcgatttc gcccttaatc gattggtatt gtttggcgag
    13021 gtcgagaaat tttatcatgt ggattgccct gtgcgaaaga ggtgaatgat cgccggcgga
    13081 gcgtatttgt caataaaata tgttcttgat ccgccgggcg gggttcccgg cggcgatgat
    13141 ccccggcgcg acgtccttcg tgaccacgct gccggcgccc accagagagc ccgcgcccac
    13201 gcgcaccccc ggcagcagcg tgcagttcgc gccgatcttc gcgttcggtt cgatgacggc
    13261 gccgcgaaga ttttgtttgg cctcggggga tttcgggtag cgcgcattgg tgatgacgac
    13321 attcggtccg atccagcact cgtccatgag ctcggtgaac tcggggatga agacctggga
    13381 gtggatgcgc acgccgctgc cgatggtcac gtggtgctcg atcaccgtga gcgtccccac
    13441 gctcacgtcg tcgccgatgc ggttgagctc gcggatgttc gccttgttgc cggtctggaa
    13501 ccccgcgccg atcacgttcc cggcgtagat caccgtatgc gagcggatca cggccccgtc
    13561 gccgatcacc gtctcctcgc cggcctgtcc ccggaagggc gccccgatga tgcagaagtc
    13621 ctccacggtc acatcggcgc ccaggcgaac gccgggatgg atgatggcgc tcggcgcgat
    13681 catcgcatgc ccagacggca cttgttgggg gaaaagcgca agaggacctc gcggcccgtt
    13741 tccatggatt cgtacatcgc cgagatgagc tccaggctcc gtcgcccctc gagaccgtcc
    13801 accagctgct tggtgttatt cgagatcgaa tcgaccacgt gctcgtagta ggcctggtgc
    13861 ccgaagccgt acacgtttgg cgggttcacg gagaacttcg agagcacctc gtcgtccccg
    13921 gcgatcggct ccgtgaagtt ccacaccttc atctcgttca ccgcgaagcc gccgatctcg
    13981 accgtgcccc gctcgcccaa tatcgagatg gacccctcga ggtccttcgg gcgcgccgcg
    14041 gtggtcgctt cgatcacgcc cagggccccg tttttaaact tgagcgtcac caccgccgtg
    14101 tcctcggtct ccacgttcac gagcgccgtg atgcccttcg cgaacacgct ctccacgtcg
    14161 cccatcatcc actcgagcag gtccacgtga tggctcgcct gattggtgag tacgccgccg
    14221 tcgaacgccc aggtgccgcg ccaggcgtcc tggtcgtagt aggcctgcgt gcggcaccag
    14281 cgcacgcgca cggtccccag aaccagtgtt ccgaagcgcc cggcctcgag cgccccccga
    14341 agtttttgca ccggaacatt gaagcggttc tgcttcacca cgaagagccg gatatcgttc
    14401 tccgcgcagg cggcgatcat gcggtcggcg tcgtcgaggg tgagcgccat gggcttctcc
    14461 acgataatgt gccggccgtg tttcgccagg cgaatgacgt gctcggcgtg gcgtccgctc
    14521 tcggtgagca ccgtgacggc gtcgatcttc tcgcgcgtca tcatctcgtc catgtccgta
    14581 tagtgcggca ccgaaaagcg ctcgccgatc gccttcgcct tttccgggac gatatcgcag
    14641 accgccgcga gctcggcgcc cgcgatctgt ccgaacccca ggagctcgga gtgccgcttg
    14701 gcgatcctcc cgcagccgac cagcgcgaac ctaagcatga tggaccacct ccccgacgag
    14761 catcgacgcc gtgatcccgc gcccgttgtg ctcgcgaatg taatcgagta tgcgaatgta
    14821 gatgtcgttc cagcccgcga actcgcggtc gtagagcgcg cggtggtgga agttcaccgc
    14881 gatagttccg ccgacgtccc tggtttcgtc caggactttt tgaatgcgcg cccaggccgc
    14941 ctcgcgatcg ccgtcgtagt ccagaacggc ggtgtccatg atcacgaggg gtatctcgat
    15001 gaggtcgaag gcggcttccg ccgccatgtc gtaggggcgg tacggatacg cgacccccgc
    15061 gcgaaagccg atggcgtcgg tgtagccgac gctgctgtcg tagacgagcc ccgcgcggac
    15121 catcacgcgc catgattcgg ggatggacag catgagatag tggttgcggc aacccgcgat
    15181 ggggcggccg atcgcgtcct cgatggcgcg ccgctcgccg atgacggcct tcggatcgtg
    15241 aaaggagtaa tagttggtgt gcaggccgac ctcccatccg gcggcggtga tgtcgccgag
    15301 catcgaccgg gcctgtgcga tcgagtagcg cgccccgaag cgccccccag cgccggtgat
    15361 catgaaaaac gttgagcgaa atccatagcg gcgctcgagg tcgatgtagg cggcgtagtt
    15421 gtcgtaggga ttgccgcgcg cgaacagtcg gcccagcgtg aacagtccca tgcgcagggc
    15481 cgatgcgatc ctgccgcgca ggagatactt caccgcgcgc gctgcccggt gtaaacaaac
    15541 atggatattc ttgtcggtga tgttgtccac gtcgtgcgag aggactacgg tgaagcgccg
    15601 cggatcggtc ctgagccgct tccccttcgc gcgggccgcc cgcgccagcg cgcgcttcag
    15661 cgtgagggcg tgacggttta ccacggggat gtcgatcgcc gcgcgttcgg tgaagcgcgc
    15721 gaggaagcgt cccttgccgt cccgccggcc gccgtactcc tccgcgcagg acagcagcgc
    15781 gaacgattcg gcgatgatgt catcgtcgat ggcgatcgcg ccgcgtcggc gccgtgcgat
    15841 cgcgggtgcg ccctccgggc ggatccacac atcgcagcgg gtatgcggtt ccgacgcgta
    15901 cgagacggca agcgcgttgt ccggcgaaac gcccgcgaca tcgagcaggg tgcggaaggc
    15961 gtattcgcac cgcgtcctgc gcgggccgtc gccgatatcg gtgatcaagc ggaccatggg
    16021 ggcgggcgcc tagggaagct tcctgaagcg gtagatcagg ttgcgctgcg tgcgcaggag
    16081 cgaaagcgcc atgcgcgtaa cggccgagct cttgcggatg atgtggtagg gccgctgttc
    16141 gcccccgaag gcgcggaaga agcgctcgat cgagggcacg gacgaaccct cgaagttgaa
    16201 ttcgcggctc accgtcgcgg cgaaccggat cgcctcccag atgacgagcg aggtcgcgcc
    16261 gctattgcgg agcgccggat cgccgccccc catgaagtag taggcgcagc gcgcatcgtg
    16321 cacgatgtag gccgcggcgt ggatgcgctt cgcctcatcg acggcgaaga gcatctttcg
    16381 cgcattgcgc gcggcgagcg tccgttcgat gcccagcacg aagtcctttc cgtagctcac
    16441 ctccatcgac tgccgctgga aggtcatccg gtgcaccgcg tagaagtcgt cgatggtcag
    16501 cgcgtcggta atggacacgc cgtttttcgc cgccttgcgc acgtcggtct tgatgttcgt
    16561 cttgaaatcg tcgaagagcg catcgaccgc ggagaggtcc tcgatcacat aggtgcaccc
    16621 gatatcgaac gagaacccgg cccatttgaa gggcagggcg ttcgtgaagt ggtagctgaa
    16681 gccatagtcg aagagcctga acctcggcag ggacgcgacg atctcctcga cgatctcgat
    16741 ctctttcgaa taccgcgtgg cgttcttcag atcagaaaaa tcccccagca gcacccccag
    16801 gacggtggtg atgggcgggt tcttgtagag cgtgccgtag tagcgcggga gcgcgatgcc
    16861 gccgatgatg cgcccgctcc cgtcctcaca ggcgaggatc gaaaagtcgg atgacaccgt
    16921 ggagagccac cagctcttat tgaacaggct cccctgcggc gatgtatcga cgaaggcgtc
    16981 ccaacgctcg tattcctcct gcgggaggat gcgtgttttt acggttcgtg ccatgtgttt
    17041 ttccgaatgg tgatgccgcc gtggtaccgg gacacggggg aaatgtcaag tccgttgtcc
    17101 ccgttgtccc cgtagagaca gggcagtgcc ctgtctctac ggggacaacg atccgcccat
    17161 gcgccatcga cgccagcgat atgctattgc cgaatcgccc aggatgagct tcgccaggcg
    17221 ccgaaggcgg atcaggacca tctccagggg gaacccgatg aggtagcgcg cccgaacgcg
    17281 aagcgggaaa tacgcacgcg ccacgcgcca ccgctcgaga tagtttcgct ctccgctcga
    17341 ataccccttc ccgccgatgc gatagcgcgc gacggggcgg tcaacgcgat agcggttgca
    17401 ggttcgcgtc gcgcccagcc gcatgaagcg gaccagccag tcgaggtcgg ccatgagttt
    17461 caggtcggca gagtaggcgc cgaccgcctc gaaggccatt cggttcgtga acagcgactg
    17521 atggcagacc ggcgtatcgt agtggaagtc gcgtagccgc agctcgcccg ccgacgacag
    17581 aatctcccga ctctgcgcgt caacgacgat cgtgtccccg tacgcgatgc cgggggcatg
    17641 cgcatcgaag cgcgcatcgc aaaacagcgc gaggacatca tcgccggcga agcggtcccc
    17701 ggcgttcatg aacgaaaacc aatcgcccgt tcccagcgcg atccccttgt tcatggcatc
    17761 gtagatgccg cggtccggtt cgcagatcca gaggtcgatg gcgtgttcat gcgcgcggat
    17821 gatgtcgaca gtcccgtcgg tcgagccgcc gtcgataacg agatactcca cattgtcgta
    17881 ggtctgctcg aggacgctgc ggatggtgtc ctcgatgtcg gccgcggcgt tgtacgacac
    17941 ggtcgcgacg gtgacgagcg ggagtcccgg gcgcgaacgc ttgacgaaac cgttcatgcg
    18001 gagcccgccg cgcatcgcgg ccgatggcgt tttgtcgtcc attccggcgg accgtgatcg
    18061 ctcgagcagg cgggtggtgg agaatggcgg tcgtgccatg atgcgcccgt ccgcctagcg
    18121 cgacggcacg gtctgtacgt accactcgta ggcgttcgcg atgccgtcgc gaagcgggat
    18181 cttcgccttc cagccgagcg cggagaggcg cgagacatcg aggagcttct ttggcgtgcc
    18241 gtcgggcttg gtggtgtcga aatacagctc gcccgtgaag ccaacgatgt ccttcacggt
    18301 cccggccgcc tcgcgaatgg tgacatcggc gccgtagccg atgttgatga attcgccgat
    18361 gtccgcgtag tcgaagtttt ccatcaagaa tacgaaggcg tcggtcacgt cgtccacgtg
    18421 caggaactcg cggaaggggc tgccggtccc ccacacggcg acgcggccct tcgtgatccc
    18481 gaaggtttcg atgagccgca tgatgtcagc gtcgctcatg gcgccgtagc ggtcggtgcg
    18541 ctcccagaag ttgaaatcgg cgcggatggc cgcgagatcg tttttttcga gcagcgtccc
    18601 caggtggaac ttgcggatga gcgcggggag cacgtgcgaa ttataaagat cgtagttgtc
    18661 gttggggccg tagaggttcg tgggcatcac cgagatgaag ttggtgccgt actgctggtt
    18721 gtagtagcgg cacagcttga tggcggaaat cttggcgatc gcatagggct cgttggtctc
    18781 ctcgagcggg ccggtgagga ggtagtcctc cttcatgggc tgcggggcga gcttggggta
    18841 gatgcacgac gagccgaggt tgaggagctt ggtgacgcca tgcgtgtggg ccgcgtgcac
    18901 gacgttggcc gcgatggcga tgttctgata gatgaactcc gctacgtagg tgctgttcgc
    18961 cgcgatcccg ccgacgcgcg cggccgacat gaagacgtat tccggcgtgt gcgtccggaa
    19021 gaactcgttc gtggccgcct ggtcggtgag gtccagcggg tattcgggaa cgcgccggtc
    19081 ccccgcattg tcaacgcggc tggtgtgcga actcgcgatg acgttcgtgt atcccttctg
    19141 aagcagggtg cggatgatgg cggcgcccac caggccgctc ccgccggcga cgaagatcct
    19201 cgagtcctta ttcatggaag cgcatcaccc gatagccgct ctcgtgcagg aggacgccct
    19261 tgcgcgcttc tctgacgtcc tcgcgaacca tctcgccaac aagatcgcgg agagagcgct
    19321 tgggcgccca gccgagctcg cgcttcgcct tcgaggggtc gccgatgagc aggtccacct
    19381 cggtggggcg gaagtagcgc gggtccacct cgacgacgat ggcgcccgtt ttcaaggact
    19441 tgagcgcctt ctcggcgagg gcggcatcat caatcgcttt catgaacgca tctttgtcga
    19501 tagcggcgat ttttcccttc tcgtggacgc cctcgccctt ccattcgacc gagacgccga
    19561 gctcgtgaaa cgacatctcc acgaaggtgc gcaccgtgtt ggtctcgccg gtggcaagga
    19621 caaagtcgcc gggcgttttc tgctggagca tgagccacat gccctcgacg tagtcgcgcg
    19681 catgtcccca gtcgcgcttg gagttcatgt tgcccaggaa catcatgttc tgcaggccca
    19741 gggcgatccg cgccgcggcg cgggtgatct tccgggtcac gaaggtctcg ccgcggttgg
    19801 gcgattcgtg gttgaagagg atgccgttgc aggcgaagat gtcgtaggcc tcgcggtagt
    19861 tcacgacgat ccaatagctg tagagcttcg cgaccgcata ggggctgcgc ggataaaagg
    19921 gcgtggtctc cttctgcggg atctcctgca ccaggccgta gagctccgag gtggaggcct
    19981 gatagaattt cgtcttgttt tcgagcttga ggatgcggat cgcctcgagc aggcgcaggg
    20041 tgccgatggc gtcggcgttc gcggtgtatt ccggcgtctc gaaggagacg gccacgtgcg
    20101 actgcgcggc caggttgtag atctcgtcgg gctgcacgtc ctggacgatg cggatgaggt
    20161 tggtggagtc ggtcaggtcg ccgtagtgga ggaagaactt tacgttggac tcgtgctggt
    20221 cctgatacag gtggtcgatg cggtgcgtgt tgaacatgga cgagcgccgc ttgatgccgt
    20281 ggatctcgta gcccttgttc atcaggagct cggcgaggta ggccccgtcc tgtcccgtga
    20341 tgccggtgat gagcgctttt ttcttcatgg ttcctcggtc ggcggtacgg attcgtaccg
    20401 gtagtattgt ctatgataac cgcccggccg cgttatccgt caagaaaaat atttgacatc
    20461 caccgggcgc gccctaggct cccgatgcaa tccatgtacg gagaaaaacg gaccaccatg
    20521 atcgatcacg gcgccctcgc gttgcccccg gagcgggtca ttatcctcga tttcggctcg
    20581 cagtacaccc agctgatcgc gcggcgcgtg cgcgagctcg gcgtgtacgc ggagatcgtc
    20641 ccctacttcc acccgctcga cgatatcacg cgcgagcgcc cctccgcgat catcctgtcg
    20701 ggcgggccct cgtcgctcta cgaggacggc gccccgcgga tcaccgccga catcttcgac
    20761 ctgggcgtcc cggtgctggg catctgctat ggtctgtatg tgatcgtgga cgccttcaag
    20821 gggacatcgc aaccggcgca ctcgaaggag tacgggcgcg cgaccatcac cattgccg
//
LOCUS       DAORHF010000036        20498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1789_length_20498_cov_5.265030, whole genome shotgun sequence.
ACCESSION   DAORHF010000036 DAORHF010000000
VERSION     DAORHF010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20498
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1789_length_20498_cov_5.265030"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKJ16_05050"
     CDS             complement(<1..354)
                     /locus_tag="PKJ16_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV46389.1"
                     /translation="MKRIHSIALITVMIIAIAGFTGTDVFAGGSSSSINLKYPVILAH
                     GYAGSDEFLGFIDYFYGIKDALKDKGVAVYAPDLGAFDTSAKRGEKLKRHILEVLATT
                     GKAKVNIIGHSQGGVT"
     gene            868..2562
                     /locus_tag="PKJ16_05055"
     CDS             868..2562
                     /locus_tag="PKJ16_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HNV46390.1"
                     /translation="MKFLSSQFTYFFQSKNTRKNIRILLKYLMALWVLIIVYSVLFHF
                     IMAAEGKEHSWVTGLYWTLTVMSTLGFGDITFSGDLGRFFSLIVLLSGVIFLLVLLPF
                     TFIQFFYAPWIEAERKSRTPRELPSHVRNHVIITSYNPVTASLIDKLTAHGREYTLVV
                     EDIQRALDFYDMGLRTSVGNIDDPETYRLMRAIEAAMVVGANSDEVNTNVAFTVREIN
                     ADVPVVCTADSTDSVDILTMAGATLVLQLSDMLGRSLARRTIGGDRRANVIGRFDELV
                     IAEAPVMGTPLVGKSLAESRLREIAGVTAVGIWERGRFAIPGPDTAITPTTVLVIAGS
                     EEQMQTYEELMCIYQVSSDRALVIGGGRVGVAVAMSLEERGIDYVVVDKSRESTGAAV
                     LKECVFGNAADIETLKRAGIERAPAAIITTHDDATNIYLTKYCRSLRPDMQIISRANA
                     DRNVSTLHRAGADFVMSYATLGASTIFNYLENADIVMLAEGLDIFRRRVPAGLMGKTL
                     AQSNIRQDTGCTVVALNNGDGMSINPDPNRPLESHGELILMGSAEAEQRFMKRFAD"
     gene            2694..3518
                     /locus_tag="PKJ16_05060"
     CDS             2694..3518
                     /locus_tag="PKJ16_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HNV46391.1"
                     /translation="MGRSIRMRLHQIMEPAVEGDRASKVFDVGIMALIVVNVLAVILE
                     TVKSIESEFGEILYRFEVFSVAVFTVEYALRLWSCVEDDRFARPVLGRLRYMVSPIAL
                     IDLLAVLPFYLPLFLALDLRFIRVLRLFRIFRLFKIGRYSESMKRMGAVFRKKKEELG
                     LAVFIVLILLVFTSCIMYYVEHEAQPEAFSSIPRAMWWGVATLTTVGYGDVYPMTAIG
                     KVLGAVIALLGVGMVALPAGIIASGFAEEIHASREEAKLVCPHCGKTFDAAKDGDA"
     gene            3646..4311
                     /locus_tag="PKJ16_05065"
     CDS             3646..4311
                     /locus_tag="PKJ16_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46392.1"
                     /translation="MKRSTVLRTMILVFAIAWTAAPAHTAPRVVEKEKDKIWQVTVDA
                     VPKTLDAFMSFRDAIAKTPQGGIVVYLVAQLIMMDNPELGEQCLIVALDRSQLAEHKP
                     GSRAVGVQGWQLGGSEKTRLGMSTFIRAKGYVAKSFVAGTSTATGYALPSLPYTYVIR
                     KHKFQDANPSVWKGNVATSCSDLGTVPIHVTVNDRGVWKVSNSSSFYAGCKPPTKKVD
                     DNL"
     gene            4357..5286
                     /locus_tag="PKJ16_05070"
     CDS             4357..5286
                     /locus_tag="PKJ16_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46393.1"
                     /translation="MTEQTDAGTGGPVSAGDSGAKRAATLTVVGLLFVFAAFIILYRA
                     ERTNNRQWIAKKAERVDPAAPGAGARMVKFTGVPGGTFLDDADSGRRFVYLRRSRYEF
                     RRGADAAAAPDWQYASGSTERAKEVRIGAVGIRLDEAEIIGGGAWSTTVYKNGREAAP
                     RIGDVKISLSGIPAELPLFCAGYFSNGYLGAGDLFVVSSFSEGKTMDELAETWVWRWL
                     RHPACFALMLVGLLLLGGPAMRTMKRHEEMPVVRSLARIGWPAYGAMSLACAFLLVRF
                     SPVTVDLIWLILALVIGVPIAITVRSVRSHRGA"
     gene            5320..5691
                     /locus_tag="PKJ16_05075"
     CDS             5320..5691
                     /locus_tag="PKJ16_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46394.1"
                     /translation="MLNHAIVKLAALGSSLGGPLFVFDLARPRLGTMPAFLLIFSPIA
                     LMMLATLALDEESTDPLARASMYGGIMGSVILFAMNEHAFWRLYQGAQHPNHGMIVAG
                     ITIGVVASALVAYRAVRFLRS"
     gene            5783..5989
                     /locus_tag="PKJ16_05080"
     CDS             5783..5989
                     /locus_tag="PKJ16_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46395.1"
                     /translation="MAKNNYQYEKRMRDLAKKKKNEEKRQRKLEKNAAKSDDATSVDQ
                     PHDDARPDAGQEIESGDVRDKATD"
     gene            complement(6115..7686)
                     /locus_tag="PKJ16_05085"
     CDS             complement(6115..7686)
                     /locus_tag="PKJ16_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HNV46396.1"
                     /translation="MAKVLLDPITRIEGHLSIEIDVQNGKVVDAKSIGNMYRGFEKIL
                     RGRNPLDANQITQRICGVCPVSHGLASSKCLDAAFGVKPNANGRLLRNLILAANYLQS
                     HIIHFYHLSALDFVDIKALLTYTGSDAKLSGLRDWVKNELETKKDRADALTVAAPFLP
                     RYEGKDFYIKDPAINLDAIAGYVKALDMRMKAHQMCTIFAGRMPHAISLVPGGVTQVP
                     TRSAIRQYRSLLKEIEKFVNSVYFNHIVAVAKAYPDYFKLGGYNNFLVFGTFEGDDAN
                     KKYLFEPAVITAGKLEKLDTARIREQVKYAWYRSGSNLHPLKGETDADPKKGGAYTWL
                     KAPRYDGKPMEVGPLARAVAAYLNPASNPYKEAIAGVLKSLQLDVSAVFSVLGRHAAR
                     VIESQILCKQAMGWLDELESGKSPRNSFDIPDVGEGEGLVEAPRGALGHWIVVKDKKI
                     ENYQCVVPTTWFCGPRDDNGVRGPVEQALIGTPISDPANPIEAARVVRSFDPCIACAV
                     HVVEGDREIAKFRVV"
     gene            complement(7679..8692)
                     /locus_tag="PKJ16_05090"
     CDS             complement(7679..8692)
                     /locus_tag="PKJ16_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HNV46397.1"
                     /translation="MTISRREFLKLMGGAAAIYAFPGVFIHGCQKMIEKAAARTNVIW
                     LQGQSCSGCSVSLLNTAKPGIASVITEHISLNFHQTVMGGTGDVAVSVLADAAAKQRK
                     DFVLILEGSIPTKAAEYCTVGVVKGKHIGIAEWTRKLGANAKAVVAVGTCATYGGIPA
                     AKDRVTKDNPTGAVGLAEYLKDIRADVVNVPGCPPHPNWMIGTLLHVLLSGVPELDEY
                     RRPTMYFGKTVHENCERLEDYKRGVYAKRWGDPGCLYNLGCLGMDSGCDIPVRKWLGG
                     VNTCTNCGAGCIGCTEPVFPDYGKRGIFTHTLASNAEIDAIEHPEVREAVLKLKNGGM
                     IHG"
     gene            complement(8705..9163)
                     /locus_tag="PKJ16_05095"
     CDS             complement(8705..9163)
                     /locus_tag="PKJ16_05095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNV46398.1"
                     /translation="MKKDVLVVGVGNILQRDEGLGVHAIAYLESEHSRFPRSVEFLDG
                     GTAGYDLIPAMKGRRKIVFIDALRADAPAGSVFRFPAERLTGAPLFSLHDAGIGTIVQ
                     MLRIEGELPAIEIIGVVPEDIASIDIGITEPVRQALPRVLEQVFDAVTTT"
     gene            complement(9165..9545)
                     /locus_tag="PKJ16_05100"
     CDS             complement(9165..9545)
                     /locus_tag="PKJ16_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HNV46399.1"
                     /translation="MNPTPAVIMLLIPFLILSCGRDPRESGVDKPRDVVSITKVTAHA
                     TKADGAPMRALVGTVKMSHRVHEEHGIGCEACHHKKGNDDRIKTCAAPGCHTGVKGFR
                     VMHGLCEDCHNARQPVREKCMRCH"
     gene            complement(9808..10728)
                     /locus_tag="PKJ16_05105"
     CDS             complement(9808..10728)
                     /locus_tag="PKJ16_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV46400.1"
                     /translation="MSSELMNTRELAEYLGIHEKQVYALIKANRLPGTRITGKWIFPK
                     HLIDEWIETHSLEGRVPAAARAPRPFEHLLAAGSNDPVLDLLLHTLKQTHPDLYIFSA
                     TTGSTEGLRLLAERNTDIAWCHLNDPADGASRVADITRALNDEKIAIVHLFQRELGLV
                     FRADCPVKKPDFAAIAEGGLRFINRQPGAGTRLVADHFFKTEGIDTRKVAGYADEVYT
                     HLEVGLAILSGAADIGLATRAISSFMGLRFAPVISESFDMILCQDVFFKKEVQAFIDT
                     LKSAEFRARVEKLGNYDFGGSGKIRYSSTQ"
     gene            complement(10895..11485)
                     /locus_tag="PKJ16_05110"
     CDS             complement(10895..11485)
                     /locus_tag="PKJ16_05110"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV46401.1"
                     /translation="MPKHRNHSFANIESYEKRFEDPARITWQKPDEVVRAMGLKDGDT
                     VADIGAGTGYFTRRIAKAVAPRGVAIGYDVERGMVEYMRADAKKLGYANYHAELVAPS
                     APALPRAGFTVIFMCNTYHHIENRPAYVRSIVPALKKAGRIVIVDSRMEAKAGPPKHF
                     RIPKEVIVAEFAKAGMRLVKDLDFLPDQHYLEFARK"
     gene            11845..14319
                     /locus_tag="PKJ16_05115"
     CDS             11845..14319
                     /locus_tag="PKJ16_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNV46402.1"
                     /translation="MRSLFTTMCTLLLCAAFSIAAIAQQPDAPADSGDKPSGKKKTSV
                     FTMGEIVVTERAIANIEQITLTTDINDKDITERGDKTLGDALRMVPGTNVYLSNKGST
                     VFDLWGLQHSKVAILVDGLPFEDVYGGGGGDISKIPMHGVSNIIVNRGISSALYGTMG
                     LIGSINVISKKPEAMFTQINAEYGQHDNYSMGIAHGAPVGNLYYMVTAGYTNSSGYEI
                     SQRLTRERREGWFNKLVQYKMYFPTLTVDDIKLDAADRYLNDSGRWSENTYQKYYASL
                     KIGYNVTKEIEVGVSSNYVLNQQHSSYFMSNTTLSFTPVSNQNTGQWYRGGAPFNAAS
                     YQTDSSSAMFRNNVFVWPEDSRYSVMPYLKMDYGDFMLRANMFYVHAVNKTEAFANQQ
                     YATNQFPTSQYYWSPTPLPAKWSSIQRSFTSTWIDTSYGINIQPSYKFAKWNRLSGAI
                     MVRNDMHDEREQALYDDETENLSATQKYWWINGMEEYETKQMEAMYLTVAVEDEMKFD
                     TPAGNLALTFGISYDAMDLRKFQAVTTDSAIMFNRLDNIDLPDDRGTTWKTATRDSYN
                     PVAGLLYEPVKDFVKLRAAYASKTEFPNLGVYASNQTAHIDYNVKPEKATNMSGGLEL
                     LFLKNTISLRGDYFYTQFKDKIERILDPTISSNRHYRNLSSVVIQGYEAAARVTLPKV
                     VDFMTNDLNFTYVHLHTINYDESYISKGDRIEKTPEHQYMAELRTKFTSGTGINIWGQ
                     HTVNQMVYVMSGLPPSGTNNKYTTSWYTKAYLHDPFFLNIRISQKIMENFEVYVMCKN
                     VLDDYQADALNPGPGRMFYFGGSGEF"
     gene            14438..15544
                     /locus_tag="PKJ16_05120"
     CDS             14438..15544
                     /locus_tag="PKJ16_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46403.1"
                     /translation="MKKLITDAARGAVLYMLLAAVAGALVPGCSKSDIEIMAVTGATP
                     SYVIAKAPGGLTLEVNGLVKRTYSFTSRSLAAFPSMAVRTREVSPKGEFEGAYFYSGI
                     PLVHIMDGVAPKKTEKSPFDKPLDIIVTFTAANGAKARFSYGELAMADDAHPVVLAYH
                     RKEIRPFKDDKKTYNKNIHRENVKGLRLICPRDSNTARYLDDVRSITFSEPDVSGLDL
                     PVTKKGAKCVSKGVTCVTGAGSAPAVYAGVPKISMDNWVRVGHGMGYKGISKAEGYRL
                     TAFLKRNNFVLSRDRFYLFVGCDGYRCIFSGWELFDNEYGSEAMLITSIDGKKPAEGP
                     MLGPVRDFFVDRDLWGFTHAVQLDDTAVADGGKK"
     gene            15541..16284
                     /gene="ccsA"
                     /locus_tag="PKJ16_05125"
     CDS             15541..16284
                     /gene="ccsA"
                     /locus_tag="PKJ16_05125"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HNV46404.1"
                     /translation="MTIGALIYGAVALYAIGLVLYLLKREQLSFGALGIGFAAHTAYQ
                     IWRAWLGGVFIPNGMVDGVFLLPWAIAGVIILLRLFGDRGTGWLSGLVPLVIFGVFAL
                     VYPKGMIPPTPNKLTLWAPTFFFTEAIGHACFYLGAWYALQSIINKSETGAFHMVVVW
                     GFVAYSLSQIVGAIWAYNGWATTFRWGSRHLQSAVIWCFYATYLHLRYMPGWGMRARA
                     RYAAVGFVVVALCTFGSHLNEMRFPRIGG"
     gene            16289..17158
                     /locus_tag="PKJ16_05130"
     CDS             16289..17158
                     /locus_tag="PKJ16_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46405.1"
                     /translation="MKRIWNFLGDVRVTFWLLIAIMATMYAGSLLASIDYKLFDGMNG
                     RAVQDWFLETGITRLAYTAWVPFLFALFGAFGINTVACTSSRFVAVLARRRSLSVRRL
                     LVLLSPSLIHVIFLVILAGHFATFVVIEQVRAPLSEKGRFELPDKSGLTVEKIEYENF
                     PDSSRLAGRVSQVRVALLPDGGGGERVVTEFTRPASYRGYDLHLDMMRKRENRDAPRP
                     AQATPMPKKSNIDDETCNKEKLYHIGHFKPTNRPELYLVATRDPGLPLLAIGFAIMLS
                     VMGFYFANYRSAE"
     gene            17184..18944
                     /locus_tag="PKJ16_05135"
     CDS             17184..18944
                     /locus_tag="PKJ16_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV46406.1"
                     /translation="MRRLLFATMIPLLALPAVFGGSGSLRSSGMRVSVSGLVGQPLTL
                     DVEDLARLRTVEVRSNEVAEDGGYRGAFGYRAVSLKTLLSLAKVRKDESDFFKPIDLA
                     VVVRNRRGERRVLSWGEVLYRQEADVVLAYDGRPVMPMKNCTACHTGSEYQKWLAPLE
                     RSIGYPKLLFPHDAVADRSLEEVDRIEVVSVPAPVRFDRKKNASSDAFSIREGNRAVV
                     VRDLSRYRPAAADAIQIGEGKGFHGRISLRGASLADIIAQNGFTPDANALLVVSSVDG
                     YRSVLSYGEVKYGRFGSGVFVADAAGGKKITELGRFHCVMPDDLASDRWVKALARIDI
                     VSIDRRGRIVIVGVGCGDARLITLDAISQIARADSVVCTDDIKERYAHYIAGKPVLFD
                     PLLHLPHYFRKKNPRHTEAESKKLVAALRERNAAQLREAIGAGKRVAFLEYGDPTIYG
                     SWTYWLFESFTSEDIDVVPGVSAFNAANAMIAKNVAVNGSVVITVPNAMIANEALLAA
                     VAKSGDTLAVFIGIKEIEAIVRLFKKHYSESTPVCLVYKAGYSESGRLYRTTLREMAA
                     VAGREKEKFLGLIYIGPAVR"
     gene            19191..19400
                     /locus_tag="PKJ16_05140"
     CDS             19191..19400
                     /locus_tag="PKJ16_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV46407.1"
                     /translation="MKISARNVIKGTVKKIIKGAVNSEIVVEIPGGSEIISVITNEST
                     EGLGLAVGKSVYAVVKASNVMIAVD"
     gene            19405..20277
                     /locus_tag="PKJ16_05145"
     CDS             19405..20277
                     /locus_tag="PKJ16_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV46408.1"
                     /translation="MKGVVGSIFLSLVFLTGCPSSGERGPGNGFILMCSTVGPVDAGI
                     VQALEEAFERESGIRMRHISAGTGAALAIARGGSIDLVMAHAKSLEEKFVSDGFGTER
                     IPLMYNDFVLVGPANDPAGVRGAARAVDALRMIAAQGSTFISRGDRSGTHIAEMDLWV
                     RAGIKPAGDWYTVYERGAEGNGPTLRFADRRSAYTVMDRATYLKHKKRIGIVVLVEKD
                     SALLNRISLIPVNPSKVARANYADVMSFVRWACDPNKGQRIIANFGTDTYGEPLYFPD
                     SREWVKSAKGKPAR"
BASE COUNT         4036 a         5752 c         6440 g         4270 t
ORIGIN      
        1 ggtcacgccc ccctgcgaat gtccgatgat attcaccttc gccttcccgg tggtcgccag
       61 gacttcgagg atatggcgtt tgagcttttc gccgcgcttg gcggaggtgt cgaaggcgcc
      121 gaggtcggga gcgtacaccg ccaccccttt gtccttgagg gcgtccttga tgccgtagaa
      181 atagtctatg aagccgagga actcgtcgct gccggcatat ccgtgggcca ggatgaccgg
      241 gtacttgaga ttgatggacg acgacgatcc gcctgcgaag acatcggtgc cggtaaagcc
      301 cgcaatcgcg ataatcatga cggtgatgag tgcgatgctg tgtattcgtt tcatgagatc
      361 ctcctgggaa gtgagtgaac cgtaaaccat ccggtcggta tgtcatgaat atagcgtatt
      421 ttgatattca ctgtcttatc aaaaaccgtc ggaacggtat ttgatcaggg gttttttata
      481 atcggggtca gagccccggt gaaatggggg tcagagcctc ggctccggcc gggtttttta
      541 tggtcatagt ttgataggtg tctaaaataa tggggtcaga gccccataat cagactcatt
      601 aggggtcaga gccctgttcg ttatcgtttc atcacactgg acgatggtgt tcttcactgg
      661 tgtgatccga tgcgcaggtg ccgcaatttt acataaacga attgacgtag gccaaaaaat
      721 ccgaatggaa tgtgattggg cgtggccgcg tccgggatac accgtgacgg tttggcgatc
      781 cattgccaaa tcatcacggg atgccgccgg gcctcatgca ggggccatgt tgatttttcc
      841 aatgcaggcg tttatgcgca cgggactatg aagtttctaa gctcacagtt cacatacttc
      901 ttccagagca agaatacacg aaagaacatc aggatactgc ttaagtatct gatggcgctg
      961 tgggttctta ttatcgtata tagcgtgctg ttccacttta ttatggcagc agagggcaag
     1021 gagcattcct gggtcaccgg cttgtattgg acgctgacgg tcatgtccac gctggggttt
     1081 ggcgatatca ccttttccgg ggacctgggg aggttctttt cccttatcgt cctgctttcg
     1141 ggagtgatct tcctgctggt ccttctgccg tttaccttta ttcagttttt ctatgcgccc
     1201 tggatcgagg cggaacgaaa aagccgcacg ccgcgcgagc tgccgtcgca tgtcaggaac
     1261 catgtcatta tcaccagcta caatcccgtg acggccagcc tcatcgacaa gctcacggcc
     1321 catgggcgcg agtataccct ggtggttgag gacatacagc gcgccctgga cttttacgac
     1381 atgggtctcc ggacgtccgt tggcaatatc gacgatccgg aaacctaccg cctgatgcgc
     1441 gccatcgagg cggccatggt ggtgggcgcc aatagcgacg aggtgaacac caatgtcgcc
     1501 ttcaccgtcc gggagatcaa cgccgacgtg ccggtcgtgt gtacggccga ttcgacggat
     1561 tccgtggaca tcctcacgat ggccggcgcg acgctggtgc tgcagctttc ggacatgctg
     1621 gggcgctccc tggcccgacg gaccatcggc ggcgaccggc gggctaatgt gatcggtcgt
     1681 ttcgacgaat tggtgatcgc cgaggcgccc gtcatgggca ccccgttggt gggcaagagc
     1741 ctcgccgaga gccgcctgcg ggagatcgcc ggcgtgacgg ccgtcggaat ctgggagcgg
     1801 gggcgcttcg ccatccccgg accggacacc gcgatcaccc cgacaacggt cctggtcatt
     1861 gccgggtcgg aagagcagat gcaaacgtac gaggagctca tgtgcatcta tcaggtgtcg
     1921 tccgatcgcg cgctcgtgat cggcggcggg cgcgtcgggg tcgcggtcgc gatgtccctg
     1981 gaggagcgcg gcatcgacta tgtcgtggtg gacaagagcc gggagtcgac cggcgccgcg
     2041 gtgctcaagg aatgcgtgtt cggcaacgcg gcggacatcg agacgctcaa gcgcgcgggc
     2101 atcgaacggg cgccggcggc gatcatcacc acccacgacg acgccaccaa tatttatctg
     2161 accaagtatt gtcgcagcct gcgcccggat atgcagatca tcagccgggc caatgccgac
     2221 cgcaatgtct cgacgctgca ccgcgcgggg gcggacttcg tcatgtcgta cgcgacgctg
     2281 ggggccagca ccatattcaa ttatctcgag aacgccgata tcgtcatgct ggccgagggg
     2341 ctggacatat tccggagaag ggtcccggcg gggctgatgg gcaaaacgct cgcgcaatcc
     2401 aatatccggc aggatacggg ctgcacggtg gtcgcgctga acaatggcga cgggatgagc
     2461 atcaatcccg atcccaaccg cccgctggag tcccatggtg agcttatcct aatgggaagc
     2521 gccgaggccg agcagcgatt catgaagcga tttgcggact agtcgggcag tggggaatca
     2581 agcggtttcg ttcgatactc atgcacggcg agattcatga tatggatgat cgcccgccat
     2641 gacgtcgagt taaccgcgaa gtgaaaatat atagagcatg aaggaggtac gcgatgggcc
     2701 gttcgattcg catgcgcctg caccagataa tggaaccggc cgtcgagggc gatcgcgcga
     2761 gcaaggtctt cgatgtcggg atcatggcgc tcattgtggt gaatgttctc gcggttattc
     2821 tggaaacggt caagtcgatc gagagcgagt tcggtgagat cttgtaccgg ttcgaggtgt
     2881 tctcggtcgc ggtgttcacc gtcgagtatg cgctgcgcct atggtcctgc gtcgaggacg
     2941 atcgcttcgc gcggccggtt ctgggccgcc tgcggtacat ggtgtcgcca atagcgctca
     3001 tcgacctgct ggccgtgctg ccgttttatc ttccgctgtt tctcgcgctc gacctgcgat
     3061 ttatacgggt gttgcggctg tttcggatct tccggctctt caagattggg cgctattccg
     3121 agtcgatgaa acggatgggc gcggtgtttc ggaaaaaaaa ggaggagctg ggactggccg
     3181 tgtttatcgt gctcattctg ctggtgttca cctcgtgcat catgtactac gtggagcacg
     3241 aggcgcagcc ggaggccttc tcgagcattc cccgcgcgat gtggtggggg gtcgcgacgc
     3301 tcaccaccgt gggctacggc gacgtgtatc cgatgaccgc gatcgggaag gtccttggcg
     3361 cggtcatcgc gctcctgggc gtcggcatgg tcgcgctccc cgcggggatc atcgcctcgg
     3421 ggtttgcgga ggagatccac gcgtcgcggg aggaggcgaa gctcgtgtgt ccgcattgcg
     3481 gtaagacctt cgatgccgcg aaggacggcg acgcatgata cgccgcggcg catcatgcga
     3541 aaatatgtat ggacattcgc cgcgcgatgg cgtatcgtta cccatggtgg ggaatgggca
     3601 tattcgatgc ccgatgaaat cggtatgcgc gtgtatggag gcgccatgaa aagatcaaca
     3661 gtgctgcgta cgatgatcct cgtgttcgcg atcgcgtgga ccgctgctcc cgcgcacaca
     3721 gcgccgcggg tggtggagaa ggaaaaggac aagatctggc aggtcacggt ggacgcggtc
     3781 ccgaaaacgc tcgatgcgtt catgtcgttt cgggacgcga tcgccaaaac cccgcagggc
     3841 gggatcgtcg tgtatctcgt ggcgcagctc atcatgatgg acaatcccga gctcggcgag
     3901 cagtgcctca tcgtcgcgct ggaccggagc cagcttgccg agcacaagcc cggatcgcgc
     3961 gcagtgggcg tgcagggctg gcagttggga ggaagcgaga agacccgact ggggatgagc
     4021 accttcattc gcgccaaggg ttatgtggcg aagtccttcg tcgcggggac atcgacggcg
     4081 accggatacg cgctcccgtc gcttccctac acctatgtga tccgcaagca taaatttcag
     4141 gacgcaaacc cgagcgtctg gaagggaaac gtcgccacct cctgctccga tcttggaacg
     4201 gtccccattc acgtgacggt caatgatcgg ggagtttgga aggtgtccaa ttcgagcagt
     4261 ttttatgcgg gatgcaagcc gcctacgaag aaggtcgacg acaatctgta gcggcggccg
     4321 gccgacggcc ggatcataca cgagaggggg gctgatatga ccgaacaaac tgatgccggg
     4381 accgggggcc ccgtgagcgc aggcgattcg ggcgcgaaac gggcggccac cctcaccgtc
     4441 gtggggctgc tgttcgtgtt cgcggcgttt atcatcctgt accgcgcgga gcggaccaat
     4501 aaccggcagt ggattgcgaa aaaggccgag cgggtcgatc ccgccgctcc gggcgccggc
     4561 gcgcgcatgg tgaaattcac cggcgtcccc ggcgggacct tcctcgatga cgccgacagc
     4621 ggacgtcgct tcgtgtacct ccggcgctcg cgctacgaat tccgccgggg ggccgatgcc
     4681 gccgccgcgc ccgattggca gtatgcgagc ggttccaccg agcgcgcgaa ggaggtgcgc
     4741 atcggagcgg tgggcattcg gctggacgag gccgagatca tcggcggcgg cgcgtggtcg
     4801 acgaccgtgt acaagaacgg ccgcgaggcc gcgccgcgca tcggcgatgt gaagatatct
     4861 ctttccggca tcccggcaga gcttccgttg ttttgcgccg ggtatttttc caacggctat
     4921 ctgggcgcgg gagatctctt cgtggtgagc tcgttcagcg agggcaagac catggacgag
     4981 ctcgcggaga cctgggtgtg gcggtggctg cgccatccgg cgtgttttgc gctcatgctc
     5041 gtggggctgt tgctcctcgg ggggccggcc atgcgcacga tgaagcggca tgaggaaatg
     5101 ccggtggtcc ggtcgcttgc gcgcatcggc tggccggcct atggggcgat gtcgctcgcg
     5161 tgtgcgttcc tactggtgcg cttctcgccg gtgacggtgg acctcatctg gctgatcctc
     5221 gcgctggtga tcggcgtccc gatcgccatc acggtccgaa gcgtgcgctc gcatcgcggc
     5281 gcctgatcgg ccgcgcgcac tcggcgggaa gcgcactcga tgctcaatca cgccatcgtc
     5341 aagctggcgg ccctgggctc ctccctcggc ggcccgctgt tcgtcttcga cctcgcgcgc
     5401 ccgcggctcg ggacgatgcc cgcgtttctg ctcatctttt cccccattgc gctgatgatg
     5461 ctcgcgacgc tcgcgctcga tgaggagtcc acggatcccc tggcgcgcgc gtcgatgtac
     5521 ggcggcatca tgggcagcgt gatcctgttc gcgatgaacg agcatgcgtt ctggcggctg
     5581 taccagggcg cgcagcaccc gaaccacggc atgatcgtcg cggggatcac gatcggcgtt
     5641 gtcgcctcgg cgctggtggc ctaccgcgcg gtgcgcttcc tgcgttcgtg accgggggcc
     5701 gctgcgagcg gattctcctt gacagatact cgtgggcggt ttcgtctctc ggctggcggt
     5761 aatttcatga aaggatatca atgtggcgaa gaacaactat cagtatgaaa agcggatgag
     5821 ggacctggcc aagaagaaga agaacgagga aaaacggcaa cggaaactgg aaaaaaacgc
     5881 ggcgaaatcg gatgatgcca cgtcagtgga tcagccgcat gacgatgccc gtccggatgc
     5941 ggggcaagag attgaatcag gagacgtgcg ggacaaagcg acggactagg gtgcgatgta
     6001 atgcgtcaat ccgcggtttt cccggtgcgg attccgctgg cgctggacct gatgaaaaaa
     6061 acgccacccg atgcttccgg gtggcggatg agaaaatccc tttaaccaga actgttaaac
     6121 gaccctgaac ttcgcgatct cgcggtctcc ctcgacgacg tgtaccgcgc aggcgatgca
     6181 gggatcgaac gatcggacga cccgcgccgc ctcgatgggg ttcgcggggt ccgatatggg
     6241 cgtgccgatg agcgcctgct ccacgggacc gcgcacgccg ttgtcgtcgc gcgggccgca
     6301 aaaccacgtc gtgggcacca cgcactgata gttctcgatc ttcttgtcct ttacgacgat
     6361 ccagtggccc agcgcgccgc gcggggcctc gaccaaaccc tcgccctcgc cgacatccgg
     6421 gatgtcgaag ctgttgcgcg ggctcttccc cgactcgagc tcgtcaagcc accccatggc
     6481 ctgcttgcac aggatctgac tttcaatgac gcgggcagcg tggcgtccga gaaccgagaa
     6541 aaccgcgctc acgtcgagct ggagcgactt gaggacgccg gcaatcgctt ccttgtacgg
     6601 attgctcgcg gggttcagat acgcggcgac cgcgcgggcg agcggaccga cctccatggg
     6661 cttgccgtcg tatcgcgggg ccttgagcca ggtgtaggcc ccgcccttct tcgggtcggc
     6721 atcggtctcg cccttgagcg ggtgcaggtt cgatccggac cggtaccagg cgtatttcac
     6781 ctgctcgcgg atgcgcgcgg tgtcgagctt ctcgagcttc ccggcggtga tcaccgcggg
     6841 ctcgaagagg tacttcttgt tcgcgtcatc gccctcgaag gtgccgaaga cgaggaagtt
     6901 gttgtagccg ccgagcttga aatagtccgg ataggccttg gcgaccgcga cgatgtggtt
     6961 aaaatacacg gagtttacga atttctcgat ctccttgagg aggctgcggt actgcctgat
     7021 tgcgctgcgc gtggggacct gggtcacgcc gccgggcacc agcgatatcg catgcggcat
     7081 gcgtccggcg aagatggtgc acatctggtg cgccttcatc cgcatatcca gcgccttcac
     7141 gtagccggcg atggcgtcga ggtttatcgc ggggtccttg atgtagaagt ccttcccctc
     7201 gtagcgcggt aaaaacggcg ccgcgaccgt cagcgcgtcg gcgcggtcct tcttcgtctc
     7261 gagctcgttt ttcacccagt cgcgcagtcc cgacagtttc gcgtcgctcc ccgtgtacgt
     7321 gaggagggcc ttgatgtcca cgaaatccag agccgacaga tgatagaagt ggatgatgtg
     7381 cgactgcagg tagttggccg ccaggatgag gttgcgcagc aggcgtccgt tcgcgttcgg
     7441 tttgacgccg aaggccgcgt cgaggcactt gctggatgcc aggccatggg acaccgggca
     7501 cacgccgcag atgcgctggg tgatctgatt ggcgtcgagc gggttgcgcc cgcgcaggat
     7561 cttctcgaag ccgcggtaca tgttgccgat gctctttgcg tcgaccacct tcccgttctg
     7621 aacgtcgatc tcgatcgaga ggtggccctc gattctggtg atgggatcaa gcaggacttt
     7681 agccatggat catacctccg tttttcagtt tgaggacggc ctcgcggact tcgggatgct
     7741 cgatggcgtc gatctcggcg ttgctcgcca gggtgtgcgt gaagatgccc cgctttccgt
     7801 aatcgggaaa cacgggctcg gtgcagccaa tgcagccggc gccgcagttg gtgcaggtgt
     7861 tcacgcctcc cagccatttc cggaccggga tatcgcagcc gctgtccatg cccaggcagc
     7921 ccagattgta caggcatccg ggatcgcccc agcgtttcgc gtacacgccg cgcttgtagt
     7981 cctcgaggcg ctcgcagttc tcgtggaccg ttttgccgaa atacatcgtg gggcgacggt
     8041 actcgtcaag ttcgggaacg cccgaaagaa gcacgtggag cagcgtgccg atcatccagt
     8101 tcgggtgcgg ggggcatccg ggcacgttca caacgtcggc gcggatatcc ttcagatact
     8161 cggcgagtcc cacggcgccg gttgggttgt ccttggtcac gcggtccttc gccgcgggga
     8221 tgccgccata ggtcgcgcag gtcccgacgg cgaccacggc cttggcgttg gcgcccagct
     8281 tccgggtcca ctcggcgatg ccgatgtgct tgcctttgac gactcccacg gtgcagtact
     8341 cagccgcctt ggtggggatc gagccctcga ggatgagtac gaaatccttc cgctgtttcg
     8401 cggccgcgtc ggccaggacg ctcaccgcca cgtcgcccgt gccgcccatg acggtctggt
     8461 gaaagttgag gctgatgtgt tcggtgatga ccgaggcgat gcccggcttc gccgtattga
     8521 gcagcgacac ggaacagccc gaacacgatt gtccctgcag ccagattacg ttggtgcgcg
     8581 ccgccgcctt ttcgatcatt ttctggcaac catggatgaa cacgccgggg aaggcgtaga
     8641 tcgccgcggc accgcccatg agtttcagga attcgcgtct gctgatcgtc atagagatcc
     8701 tccgttatgt ggtggtaact gcgtcaaaaa cctgctccag cacccgtggg agcgcctgtc
     8761 gcaccggttc cgtaatgcca atgtcaatgc tggcgatgtc ctcggggacc acgccgatga
     8821 tctcgatcgc cggcagctcg ccctcgatgc ggagcatctg cacgatggtg ccgatcccgg
     8881 cgtcgtgaag cgaaaacagc ggcgcgcccg tcaggcgctc ggcgggaaag cggaacacgc
     8941 tcccggccgg cgcgtcggcg cgcagggcgt cgatgaacac gattttgcgg cggcccttca
     9001 tggccgggat gaggtcgtat ccggccgtgc ccccgtcgag gaactcgacc gatcggggaa
     9061 agcgcgagtg ctcgctctcg agataggcga tggcgtgaac gcccagcccc tcgtcccgtt
     9121 gcaggatgtt tcccacgccg acgaccaaca cgtctttctt cacgtcagtg gcaccgcatg
     9181 catttttcgc gcaccggctg gcgcgcatta tggcagtcct cacagagccc gtgcatcacc
     9241 cggaatccct ttacgccggt gtggcatccg ggcgccgcgc aggtcttgat ccgatcgtcg
     9301 tttcccttct tgtggtgaca ggcctcgcag ccgatcccgt gctcctcgtg gactcggtgc
     9361 gacatcttga ccgtacccac cagggcgcgc atcggcgcgc cgtcggcctt ggtcgcgtgg
     9421 gcggtcacct tggtgattga tacgacgtcc cggggcttgt cgacgcccga ttcgcggggg
     9481 tccctgccgc acgaaaggat gagaaacgga ataagcagca tgatgacggc gggtgtcgga
     9541 ttcatggctt tcacgcaggg cctcctgtgt tactcacggt tctcggtgtt gtgaaggtgg
     9601 tggcaatgtg cttaaatgag cacgatcaac aatgcatatg acctgattgt cgagtcaatc
     9661 aaaaagtatc taaatattac aaataattac aaaaagaaac ctataaacgc acgaccggcc
     9721 agcatggact ggatccggaa gctgttgcac cggaatgtag tgaccgatgg cgatcagtga
     9781 agggtggggc gacagtcgac gacggattta ctgtgtgctc gaataccgga tcttcccgga
     9841 gccgccgaag tcgtaattgc cgagcttctc gacgcgcgcg cggaattcgg cggacttgag
     9901 agtgtcgatg aaggcctgga cctctttctt gaagaagacg tcctggcata ggatcatatc
     9961 gaagctctcg ctgatgaccg gcgcgaaacg caggcccatg aagctcgata tggcccgcgt
    10021 cgcgagcccg atatcggccg cgcccgagag gatggcgagg cccacctcga gatgggtgta
    10081 cacctcgtcc gcgtaaccgg ccaccttccg cgtatcgatc ccttccgttt tgaagaaatg
    10141 gtccgcgacc aggcgcgtgc ccgctcccgg ctgccggttg atgaatcgca atcctccctc
    10201 ggcgattgcc gcgaaatcgg gctttttcac cgggcagtcg gcgcgaaaga cgagccccaa
    10261 ctcgcgctgg aacaggtgaa caatcgcgat cttctcgtcg ttgagcgcgc gggtgatgtc
    10321 cgcgacccgc gacgcgccgt cggcggggtc gttgaggtgg caccaggcga tatcggtgtt
    10381 tcgttccgcg agaagacgca ggccctcggt gctgccggtg gtggccgaaa agatatagag
    10441 gtccgggtgc gtctgcttga gggtgtgcag caggaggtca aggacggggt cgttgctgcc
    10501 ggcggcaagc aggtgttcga agggacgcgg cgcgcgggcg gcggccggca cgcggccctc
    10561 gagcgaatgc gtttcgatcc actcgtcgat caggtgtttc ggaaaaatcc atttcccggt
    10621 gatccgggtg ccggggagcc ggttcgcctt gatgagggca tacacctgct tctcgtggat
    10681 gccgaggtac tcggcgagtt cgcgcgtgtt catcagttcg ctggacatgg ttcgctcctg
    10741 ggtatgtcca gtctgttgcg gaaaaaaggt tttcgtcaat acaaaatagt acggctacgg
    10801 cgttatccgc cgccagccgg caggacgcgc tccccatcac ggccaccgca gcggggggtg
    10861 atggggtttt gaaacgcgac ggaatcgcgg agcgctattt tcgcgcgaat tccaggtagt
    10921 gctggtcggg caggaagtcc aggtctttga cgagccgcat gcccgccttc gcgaactcgg
    10981 cgacgatgac ctccttggga atcctgaagt gcttcggcgg ccctgctttc gcctccatgc
    11041 ggctgtccac gatgacgatg cgccccgctt ttttaagcgc tggcacgatg gaccgcacat
    11101 acgcggggcg gttctcaatg tggtgatagg tgttgcacat gaagatgacc gtgaacccgg
    11161 cccggggaag cgccggcgcg ctaggggcca ccagttccgc gtggtaattg gcatagccca
    11221 gttttttcgc atcggcgcgc atgtattcca ccatgccccg ttcgacgtcg tagccgatgg
    11281 ccacgccccg cggggcaacg gccttcgcga tccgccgcgt gaaatagccc gtccccgcgc
    11341 cgatgtcggc gactgtgtcc ccgtccttca ggcccatcgc gcgtaccacc tcgtccggtt
    11401 tctgccaggt gatgcgtgcc gggtcctcga agcgtttctc gtaggattcg atattggcga
    11461 aggaatggtt acggtgcttt ggcatgtcat ttccatggct gcacgatgct atgataagcg
    11521 ctatcagtgc gagcggcagc aagagatgaa aagagcttcg aatcataggg ggcacctcgt
    11581 tgtggatagt agttttctgg gccattaaga gatggcaata tcgcaactgg caataaaaaa
    11641 cacaaaaaat tatccaaata cgtcttatat tataatcatt tatattaata tgattatatt
    11701 atatttaatt aaatttaaaa aaacaatatt tcatgtaata ttctgtaact aaatgtaata
    11761 ttattcattg actcgaaata attcccatcg aaccatggcc cattcacaga atcagttaaa
    11821 tctaacacaa tacaggtgaa tggcatgaga tcacttttca caacgatgtg cacgctactc
    11881 ctctgcgccg ccttttccat cgcggcaatc gcgcagcagc ccgacgcgcc cgccgacagc
    11941 ggcgacaagc cctccggcaa aaagaaaacc agcgtcttta ccatgggcga gatcgtggtc
    12001 acggagcggg ccatcgcgaa catcgagcag attacgctga caacagacat taatgacaaa
    12061 gatatcaccg aacgagggga taaaaccctc ggagatgcgc ttcgcatggt tcccggcacg
    12121 aacgtatatc tttccaataa gggcagtacg gtgttcgatc tctgggggct ccagcattcg
    12181 aaagtcgcca ttctggtgga tggccttccc tttgaggacg tctatggcgg cggcggtggg
    12241 gatatctcga aaatcccgat gcatggcgtt tcgaacatta tcgtgaaccg cggtatttca
    12301 tctgcgctgt atggaaccat gggactcatc ggttccatca acgttatctc gaaaaaaccc
    12361 gaggcgatgt tcacgcagat caatgcggag tacggtcagc atgacaatta ttccatgggt
    12421 atcgcacacg gggcgcccgt gggaaacctg tactatatgg taacggccgg atatacgaat
    12481 tcctccggtt atgagatatc gcagcgcctt acccgcgaac gccgcgaggg atggttcaat
    12541 aagctggtgc agtacaagat gtatttcccg acgcttactg ttgatgacat caaactcgac
    12601 gcggccgatc gctatctgaa cgatagcggg cgctggtcgg agaacaccta tcagaagtat
    12661 tatgcttccc tcaagatcgg gtacaatgtg acgaaggaga tcgaggtcgg ggtgtcttcc
    12721 aattacgttc tcaatcagca gcattcaagt tatttcatgt cgaatacaac attgagtttt
    12781 acgccggttt caaatcaaaa tacaggtcag tggtacagag gcggtgctcc gtttaacgca
    12841 gcttcatatc agaccgattc aagtagtgcg atgttcagga ataacgtgtt cgtctggcct
    12901 gaagactcgc gttattcggt catgccgtat ctcaaaatgg attacggcga tttcatgttg
    12961 cgagccaata tgttttatgt gcatgccgtc aataaaacgg aggcgttcgc aaatcagcaa
    13021 tacgctacta atcaattccc gacgagtcag tattattggt ctccaacgcc actaccagca
    13081 aaatggtcaa gtattcagag atcgttcaca tcaacatgga tcgatacctc gtatggtata
    13141 aatatccaac cgtcgtacaa gtttgcgaaa tggaaccggc tctccggcgc gatcatggtc
    13201 cgcaacgaca tgcatgatga gcgcgagcag gcactgtatg acgacgaaac cgaaaatctg
    13261 agcgcgaccc agaagtattg gtggatcaac ggcatggagg aatacgaaac caagcaaatg
    13321 gaagcgatgt acctaacggt ggcagtcgag gacgagatga aattcgacac accagcagga
    13381 aatctcgcgc tcacgttcgg aatatcgtac gacgccatgg acctgcggaa attccaggcg
    13441 gtgacgaccg attcggccat catgtttaat cggttggata atatcgatct gcccgatgat
    13501 cgcggaacga catggaaaac ggcaacgcgc gattcataca atcccgtcgc cggattgctc
    13561 tatgagccgg ttaaggattt cgtgaaactc cgtgcggcat acgcttcaaa aacggagttc
    13621 ccgaaccttg gcgtgtatgc gtcaaatcaa acggcgcata tcgattacaa tgtaaaaccg
    13681 gaaaaggcga ccaatatgag tggcggtctt gagcttttat tcctcaagaa taccatcagc
    13741 ctgcgcgggg attatttcta tacgcagttt aaggacaaga ttgagcgcat tctggatccc
    13801 accatttctt cgaatcgcca ctaccgaaac ctgtcgagcg tggtcataca ggggtatgaa
    13861 gcggccgcac gcgttacgct tcccaaggtt gtcgatttca tgacgaacga cctgaacttt
    13921 acctatgtgc acctgcatac gatcaattac gacgaatcct atatttcgaa gggagatcgg
    13981 atcgaaaaaa cgcccgaaca ccagtatatg gccgagctca gaacaaagtt cacatcgggc
    14041 accggtatca acatctgggg ccagcatacc gtgaatcaga tggtgtatgt gatgagcggg
    14101 cttcccccat caggcaccaa taacaagtac acgacctcgt ggtacacaaa ggcttatctc
    14161 cacgacccgt ttttcctcaa catccgcatc tcccagaaga tcatggagaa tttcgaggtc
    14221 tatgtgatgt gcaagaacgt gctagacgac taccaggccg atgcgctcaa cccggggccg
    14281 ggacgcatgt tctacttcgg cggcagcggc gagttctagt agcgccgtcc atatcagtag
    14341 aggtggaagg ttttttacga gtgtgatcga atccgccccg cgggagtgcc gcggggcgga
    14401 ttagtatatg ccgcgcgaaa atacaaggag agagatgatg aaaaaactga taacggacgc
    14461 tgcacgggga gcggtgttat atatgctgct ggcggctgtt gccggcgcgc tagtgcccgg
    14521 ttgcagtaaa agcgatattg aaatcatggc ggttaccggc gccacgccga gttacgtgat
    14581 cgcgaaggcg cccggcggcc tcacgctcga ggtgaacggg ctggtgaaaa ggacgtattc
    14641 cttcaccagc cggtcgctgg ccgcgttccc gtccatggcg gtgcggaccc gcgaggtctc
    14701 gcccaagggc gaattcgagg gggcctattt ctattcgggc attcccctgg tgcatatcat
    14761 ggacggcgtg gcgccgaaga agaccgagaa gtcgcccttc gataagccgc tggacatcat
    14821 cgtcaccttt accgccgcga acggcgcgaa ggcgcgcttt agctacggcg agctcgcgat
    14881 ggccgacgac gcgcatccgg tcgtgctcgc gtatcaccgt aaggagatcc ggccgttcaa
    14941 ggacgacaag aagacctata acaagaacat tcatcgcgag aacgtgaagg ggctgcggct
    15001 catctgtccg cgcgattcga acaccgcgcg ctatctcgac gacgtgcgct ccatcacctt
    15061 ctcggagccg gacgtgagcg gcctggatct cccggttacc aaaaagggcg cgaaatgcgt
    15121 ttcgaagggc gtcacctgcg tcacgggcgc ggggagcgcg ccggcggtgt acgccggggt
    15181 gccgaaaata tcgatggata attgggtgcg cgtgggacat ggcatgggtt acaaaggcat
    15241 atcgaaggcc gaaggatacc ggctcaccgc gtttctcaag cgaaacaact tcgtgctcag
    15301 tcgcgatcgg ttttatctct tcgtcgggtg cgatggatac cggtgcattt tttccggttg
    15361 ggagctgttc gacaacgaat acggcagtga ggcgatgctg atcacttcaa ttgacggaaa
    15421 gaagccggcg gagggaccca tgctgggacc cgtgcgcgat ttctttgtcg atcgcgacct
    15481 gtggggcttc acgcacgccg tgcagctgga cgataccgcg gtagccgacg gagggaagaa
    15541 atgaccattg gcgcgttgat ctacggtgcc gttgcgctgt atgccatagg gctggtcctg
    15601 tatctcttga aacgcgagca actgtcgttt ggcgcgctcg gaatcgggtt tgcggcgcac
    15661 acggcgtatc aaatctggcg ggcatggctg ggcggcgtgt ttattcccaa tggcatggtg
    15721 gacggcgtgt tcctcctgcc atgggccatc gcgggggtca tcatcctgtt gcgacttttc
    15781 ggcgatcggg gaacgggctg gctctcgggg ctcgtgccgc tggtcatctt cggggtcttc
    15841 gcgctcgtct acccgaaggg catgatcccg cccacgccca acaagctcac gctgtgggcg
    15901 ccgacgtttt ttttcacaga agcgatcggg cacgcgtgct tctatctggg ggcgtggtac
    15961 gcgctgcaga gcatcatcaa caagagcgaa acgggcgcgt ttcatatggt cgtcgtgtgg
    16021 ggcttcgtgg cctacagcct ctcgcagatc gtaggcgcga tctgggccta caacggctgg
    16081 gccaccacct tccgctgggg ctcgcgccac ctgcagtcgg cggtcatctg gtgcttttac
    16141 gccacctatc ttcatttgcg gtatatgccg ggatggggga tgcgggcgcg cgcgcgatac
    16201 gccgctgtgg ggtttgtcgt cgttgcgctg tgcacattcg ggtcgcactt gaacgagatg
    16261 cggttcccac ggatcggggg gtgatgtaat gaaacggata tggaactttc tgggcgacgt
    16321 tcgcgtgacc ttctggctgc tcattgccat catggcgacg atgtacgcgg ggtcgctcct
    16381 cgcatcgatc gattacaaac tgttcgacgg catgaacggc cgggcggttc aggactggtt
    16441 tctggaaacg ggcatcacgc ggctggcata caccgcttgg gtgccgttcc tgttcgcgct
    16501 ctttggcgcc ttcgggatca acaccgtcgc ctgcacttcg agccggttcg tggcggttct
    16561 ggcgcggcgg cggagtctct ccgttcggag attgctcgtg ctcctgagcc cgtcgctcat
    16621 ccatgtgatc tttcttgtga tcctcgccgg gcatttcgca accttcgtcg tcatcgaaca
    16681 ggtgcgcgcg ccgctttccg agaaggggcg gttcgagctc cccgacaaat cggggctcac
    16741 cgtcgagaag attgaatatg aaaatttccc ggactcctcg cggctcgccg gcagggtgtc
    16801 gcaggtgcgc gttgcgctgc tcccggacgg cgggggcggc gagcgcgtgg tgacggagtt
    16861 cacgcggccg gcctcgtatc gcggatacga cctgcatctg gacatgatgc gcaagcgtga
    16921 gaaccgtgac gcgccgcgac ccgcgcaggc cacaccgatg ccgaagaaat ccaatattga
    16981 cgatgagacc tgcaacaagg agaagctcta tcatatcggg cacttcaagc ctacgaaccg
    17041 cccggaactc tacctggttg ccacccgcga tcccggcctg ccgctgctcg caatcggctt
    17101 cgcgatcatg ctgtccgtga tgggatttta cttcgcgaac tatcgcagcg ccgagtaggc
    17161 gtcggcgcat acggagaagc gcgatgagac gattgctatt tgcgacgatg attccccttc
    17221 tggcgctgcc ggcagtattc ggcggctccg gttcgcttcg gtcctcgggg atgcgtgtga
    17281 gcgtttcagg actcgtcgga cagccgctca cgctcgatgt cgaggacctc gcccgcctgc
    17341 ggacggtcga ggtgcgctcg aacgaggtcg ccgaggacgg cgggtatcgc ggggcgttcg
    17401 gctatcgcgc ggtatcgctg aagacgctgc tgtcgctcgc gaaggtgcgg aaggacgaga
    17461 gcgacttttt caagccgata gacctggcgg tggtcgtacg caaccgtcgg ggggagcggc
    17521 gcgtgctctc ctggggcgag gtgctgtacc gccaggaggc cgatgtggtt ctcgcatatg
    17581 acggcaggcc ggttatgccg atgaagaact gcactgcctg ccacacgggg agcgaatacc
    17641 agaaatggct cgcgccgctc gagcggtcga tcggatatcc caagctgctc tttccgcacg
    17701 acgccgtcgc cgatcgctcg cttgaagagg ttgaccgcat cgaggtggtt tccgttccgg
    17761 cgcctgttcg cttcgacaga aagaagaatg cgtcatccga tgctttttca atacgcgagg
    17821 ggaatcgcgc cgtcgtggtc cgcgacctgt cgcggtatcg ccccgccgcg gcggacgcga
    17881 tccagatcgg cgaggggaag gggtttcatg ggcggatctc cctgcgtgga gcgtcgctgg
    17941 cggacatcat cgcgcaaaac ggtttcacgc ccgatgcaaa cgccctgctg gtggtatcct
    18001 ccgtcgacgg ctatcgttcc gtgctgtcct acggcgaggt gaagtacggg cgcttcggaa
    18061 gcggcgtgtt cgtggccgac gcggccggcg ggaagaaaat caccgaactg ggaaggttcc
    18121 actgcgtgat gcccgacgac ctcgcctcgg accgctgggt gaaggcgctg gcccgcatcg
    18181 atatcgtttc tattgacagg cgcggccgca ttgtcatcgt gggcgtgggc tgcggcgatg
    18241 cgcggctcat cactctggac gcgatctcgc agatcgcccg cgccgacagc gtcgtgtgca
    18301 cggacgacat caaggagcgc tacgcgcact atatcgccgg caagccggtc ctgttcgatc
    18361 ccctgctgca ccttccgcat tatttcagaa agaagaaccc gcgccataca gaggccgaga
    18421 gtaaaaaact ggtcgccgcg cttcgcgagc ggaacgcggc gcagcttcgg gaggccatcg
    18481 gcgcggggaa gagagtcgcg tttctcgagt acggcgatcc gaccatatac gggagctgga
    18541 cctactggct gtttgaatcg ttcacttcgg aggacatcga cgtggtcccc ggcgtgagcg
    18601 ccttcaacgc cgccaatgcg atgatcgcga agaacgtcgc cgtgaacggg tccgttgtca
    18661 tcaccgtgcc caatgcgatg atcgcgaacg aggcgctgct cgcggcggtc gcgaagagcg
    18721 gcgacacgct ggcggtgttt atcggcatca aggaaatcga ggcgattgtg cgattgttta
    18781 agaagcacta ttcggaatca accccggtgt gcctggtcta caaagcggga tattccgaaa
    18841 gcggacggtt ataccggacg acactgcggg agatggccgc cgtagcgggg cgggaaaagg
    18901 aaaagttttt ggggcttatc tatatcggac ccgccgtgcg gtgacgcgtt cgcttcgcgg
    18961 ggcgccggta aaaaaagccc tcccatttgg agggagggcc gatactaccg cagtgtttag
    19021 agagagaatt gcgtgtttaa aaaatatatt acagaaacct actaattctt acatgcaata
    19081 tagtcataat tatttggata tttattaatg caagagaaaa ttattgaccg ggattgtttt
    19141 tgtatacatg atagtgctgt agtgaaatcc tatgtacaag ggtgggcgat atgaagatca
    19201 gcgcgcggaa cgtaatcaag ggcacggtaa agaaaatcat caagggcgcg gtcaattccg
    19261 agattgtggt cgagatcccc ggaggatccg agatcatctc cgtcattacc aatgaatcca
    19321 ccgaggggct cggtcttgcc gtcggtaaaa gcgtctatgc cgtcgtcaag gcttcaaatg
    19381 taatgatcgc cgtcgattga ggccgtgaag ggagtcgtcg gctcaatctt cctgtcgctt
    19441 gtttttctca ccggatgccc atcgtccggc gagcgggggc ctggcaatgg attcattctc
    19501 atgtgcagca ccgtcggccc cgttgacgcc gggatcgtcc aggcgctcga agaggccttc
    19561 gagcgggaga gcgggatccg catgcgccac atcagcgcgg gcaccggcgc ggcgctcgcg
    19621 atcgcgcggg gcgggtcgat cgatctcgtg atggcgcatg cgaaatccct cgaggagaaa
    19681 ttcgtgagcg acggattcgg cacggagcga attccgctca tgtacaatga tttcgtcctt
    19741 gtggggccgg cgaatgaccc ggcgggcgtc cggggagcgg ccagggccgt cgatgccttg
    19801 cggatgatcg cggcgcaggg ctcgactttc attagccggg gtgaccggag cggaacgcat
    19861 atagccgaga tggatctctg ggtcagggcg gggatcaagc cggcgggaga ctggtatacg
    19921 gtgtacgaga ggggcgcaga gggcaacggg cccacgctta ggttcgcgga tcgaagatcg
    19981 gcgtacaccg tcatggatcg ggcgacgtat cttaagcata agaaacgtat cgggattgtt
    20041 gtccttgtcg agaaggactc agcgctcctg aaccgcatct cgctcattcc ggtcaatccg
    20101 tcgaaggtcg cccgggcgaa ctatgcggat gtcatgtcct ttgtcaggtg ggcctgcgat
    20161 ccgaacaagg gccagcggat catcgcgaat ttcggtacgg acacctatgg cgagcccctc
    20221 tatttcccgg attcgcggga atgggtgaag tccgcgaagg gaaaacctgc acggtgaatg
    20281 caaaagcaat cgattgtcaa tatcacggat ggtgcagcat gagcgtcaat gacagtatga
    20341 atcacacgaa acgcaggaga tgatacggag cgtgtacaca ctctctccat ccaccgacgt
    20401 cctccggctc cgaaaccgcc tgcggctcct tgcgtggctg gccctctcga tcgcgctcca
    20461 tctggcgatc ctcgcggcgc tgcgctcggt ttcgacgc
//
LOCUS       DAORHF010000037        20355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1809_length_20355_cov_6.173448, whole genome shotgun sequence.
ACCESSION   DAORHF010000037 DAORHF010000000
VERSION     DAORHF010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20355
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_20355_cov_6.173448"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PKJ16_05150"
     CDS             <1..794
                     /locus_tag="PKJ16_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46409.1"
                     /translation="AESLLDVYLSQDKQAPKELRELERALEGYNIILRFTRDVSSANK
                     RLILDYCIYGRKKLIKISHPLFNMREKVYNIQPLIYYDEFSTSNSTFYFDMIYINPEE
                     VHNDYVIAKNVLAGKSVKSMFFVGARVSEDIASCLRRAFAGGRPIKPEILRMFEIHEL
                     THKILNNHYNFYDQVIGEELALSSTIYANPFLGLSVLYSYLDYSVINPHRIAAMNFVR
                     FAASRTRNTDMIDNPSLMRALGEEEILKLAREHFNSIIKTLRRSN"
     gene            complement(791..1882)
                     /locus_tag="PKJ16_05155"
     CDS             complement(791..1882)
                     /locus_tag="PKJ16_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HNV46410.1"
                     /translation="MPGIISHHRLLVETVAHLARERHLPPPLRSVEILLNTPAFKNAA
                     FFGAIGPNIFDYAPGLTRQLYGSPLSYLMHNGGSVTFIAAMLRRIGALDDLNTEWASV
                     QRAYLHGFIAHAVADIVYHPFMFYWSGFPDAFIRREINHYREQNLVFEYNLDLFFLHQ
                     YRTDGYAFNLDDMLPLREGRRGLRINRPVKRLILEAFKEGFPDQYRRLFLGRPSGDVD
                     GDANAFTLLDALPSLIRATYWIKRNRNPRLNALLDALRRRTALFNDYLVRYPDPRRIN
                     SHVLNLHRQRWVYPAGARGIHYESVEDLMRVVVDRTVDLWARVEAKLRQGNDNYSDVI
                     RDLRTNNLSGEDGKDYLAMKDKEPVRLRV"
     gene            complement(1887..2120)
                     /locus_tag="PKJ16_05160"
     CDS             complement(1887..2120)
                     /locus_tag="PKJ16_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46411.1"
                     /translation="MTRKAILAGIALVFVGTALYSSPAIMNLHRGLKRAGVEVVKSCA
                     DCHNAATGLKQAKGQDVKRLYRTPACTGKGCHL"
     gene            2540..2905
                     /locus_tag="PKJ16_05165"
     CDS             2540..2905
                     /locus_tag="PKJ16_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HNV46412.1"
                     /translation="MANAKSSNNKTKVAYPEKYTVNTISDYLAKKHGIPKRQAKEMLE
                     DMFAVINSGVMSGERVPIGKMGKMFIRVRPATKARQGRNPLTGEEITIPAKKATKVPK
                     FAFTKTFKTEATKAKVKTK"
     gene            complement(3093..4286)
                     /locus_tag="PKJ16_05170"
     CDS             complement(3093..4286)
                     /locus_tag="PKJ16_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46413.1"
                     /translation="MNTPIEQPRRDHSIDEATALKIALYYTKGDVESANNMVAGSYND
                     LQVFKIKFTSPMINGACLLFFNRIYNQLADCYGIISPTYSVGDISTHKDWKEFEREIS
                     EYFNTCEHDKGQVFSFLEAVRNGVTATFAKDLQYFVEMQDLPGMASIFKRQVFSGIEL
                     GNIKLDVHFETISSLDMERYSKSSIKLTPEQISGAQPPAADGSMADDAPIVIEKDDED
                     TFDKNKIKLIFHGALVLDPINGMNVAGLVVKDSIKIRLVDENRKAVLIAKAFNAYADG
                     QMMPIVGSIVSYRKKATGGYKIFVEIADGIYVRIEEDERNIKVAFVAPSPDRPPELQK
                     GIEKYVDKYIEQQRKKEPVNDDDGKAAGAADGADEGNIYTQLAAFAVILLCIIGVIVF
                     IVMKK"
     gene            4573..5301
                     /locus_tag="PKJ16_05175"
     CDS             4573..5301
                     /locus_tag="PKJ16_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNV46414.1"
                     /translation="MTAQPAHLDPVSKNRLHDEIVRQFQRKIVRGEFAVGQKLPPERE
                     LAASLAVNRATLREALKKLEVLGLLQILHGDGIYVKDFRASGNLELFKALVYLDSVIN
                     ADILRNMLDIRKLITPEMAARAAHNRSEADLARLAHIVEGENPADILERDLAVHNGIA
                     LSGGNLCYLFIHNFFSGIIRDFGHLYFADPANVERSRRFHRDILEAIRKKDAKRAHRV
                     MHDVLDYSEKSIYAQYDARYNTHS"
     gene            5313..6944
                     /locus_tag="PKJ16_05180"
     CDS             5313..6944
                     /locus_tag="PKJ16_05180"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HNV46415.1"
                     /translation="MERFLDTYRGARFDIIVVGGGITGAAVAYEAASRGLSVALLEKN
                     DFGWATSAATSKMIHGGLRYLNNFELGLVRESLRERRILENIAPNLVYPFPLLIPNYK
                     KLMTRKSVLNFGLTLYDILAFDKKRTWDPSKRIPNHSTYSRDKTRAIAPGVKTDGLTG
                     SCVFYDCRSIFPERLTLAFVRSAMRYGAKAANYATVTGFVRGPGGRIAGVTVKDTVKR
                     REMEIHGELVVNCGGPWADIVLNTAKGGANDHPMKRSEGIHIITKKLYNEHAVTLQTP
                     EGRHFFILPWRNHALIGTTDKEFFGSPDDYRVSRQSVQELIDEVNQSYGDGTLSYDDV
                     LFAYGGLRPLVEQQTAETYESSRKYEIHDNAEEGFEGLITVEGGKYTTSRNLADNVLK
                     MVKIKLHRSLTTSITDRIYLHGCEIKDLEEFVRQVHTAHADFPKNTREYLARNYGTEC
                     HAVFDIARENTALAQPVNGDGEILAQVAYAARREMALTLNDILFRRTGLGTLGDPGAK
                     VLNAAAKVAAKELRWNAARVKKEIAAAKKALALPA"
     gene            6978..8606
                     /locus_tag="PKJ16_05185"
     CDS             6978..8606
                     /locus_tag="PKJ16_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV46416.1"
                     /translation="MYGNYRHIFKWGDPSHEEQLKDHVVHFAKEHFGMTDADFANKHL
                     PGFDTITLKKRPRLSKASLGALKKIVGAENVSTEDFERAYHSAGKFYLELLKLRLGVI
                     DNPPDAVVYPRDEKDVIAIVRLCNAKRIPITPFGGHSSVTRGVEPVKGGISLDLTRHM
                     NAVIRVNEINSTVTVQPGMFGPALEEFLNRYKAGYTCWHFPQSFEFSTVGGWAVTRGA
                     GQASTGYGKIEDMVLSMRTVTPAGIIETKDYPAAAIGPDIDQIIIGSEGAYGVVTEIT
                     MKIRTYRPQNTSHTSFVFKDFKSAVTAMRTVMQAEFGKPHLFRISDPEETEMAFRMGG
                     KHGGLGDKVLSLLGYKPMQRCLMFVSVEGDANYTKFVVGKIKDVASDFGALCTGAGPT
                     KKWLEQRYSSAYLRDPLMDAGILADTLETSVSWENLNQLWSEVRDYIKARPKTVCMVH
                     LSHIYENGANLYFTFLTPMNKGDEVRDFVQFHKGIVDTVHANHGSISHHHGVGKMLAP
                     WMPKEHDTASIELMRAIKKHLDPKGIMNPGTLGL"
     gene            8804..9490
                     /locus_tag="PKJ16_05190"
     CDS             8804..9490
                     /locus_tag="PKJ16_05190"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HNV46417.1"
                     /translation="MNGYRTAAIICLLIIIPLSIQAESDDARKLRNAMETLAQIAAIP
                     EGGIPTALLANARGIAVLPGTVKAGCVLGGRYGTGVLSIQNADGTWSLPAFITLAGGS
                     LGWQIGAQGTDIILVFKSRKGIDAIGNGKLTLGADIAIAAGPVGRQASAATDIELKAE
                     IYSYSRARGLFAGLALEGALLRLDEKANHAWYGTGSNPESILTAAPPRVPEEAKGFSA
                     ELKKLMVPRR"
     gene            9561..10895
                     /locus_tag="PKJ16_05195"
     CDS             9561..10895
                     /locus_tag="PKJ16_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46418.1"
                     /translation="MDVTLVIVLALGAIWMAYAVKMSRRIYSHTDLMMKQTDELVIVP
                     ASGAAPEVVIHLVNGWNVTGEYNADGEANLVSIRIEAPKPCATSFTISRETAASRFKK
                     NLGMDDIAVGDDRFDDECLCKSNNTLFLLSRLGSALRSRLLAFADRADEFTITHAGFT
                     ARFSAVTGERPAFIDRTAEEMIAIHRAITGSPATKPRLKSIIFDDPIAHVRLRCLEAY
                     AAQFGLDASFERCLADADIGVQIAAARHLGEKGLSHLATLIGSQPDIADEDKTEIIRF
                     CNERTHPLPGTILATLLDGAHHRDLLSEAIGACRFSSDPSLSEHLLRRLERAGLDDLL
                     RLEIILVLGDCGTLAAVEPLYRIAENRRLSSGPRTAANEAIARIQSRQGTGERGWLSV
                     STPAGKEGALSITRGAGQGALSSPNAAPTVKSTARLKTGNRRSSKKTSKKKK"
     gene            10900..12210
                     /locus_tag="PKJ16_05200"
     CDS             10900..12210
                     /locus_tag="PKJ16_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46419.1"
                     /translation="MTWFLVIVGGLVAFLLIQLVMRWLDSPPMLTVADEPFKSLLSDV
                     RTDNGAMRGTYRGHPAIVSFTYEKSRSYLFEVITTIVRMDIPPHITGPFSVSRETVLS
                     NSKRLIGKGDYQTGDVRFDRTFLISSDHPVVLPALLNAAVRKNLLKLNSSAYALSLSN
                     EQFMAELPRPSKFGKAIRNAFETMASIIDDIASPASIRDRLVSSILHDPVAAVRRNCL
                     EALIANVPIDTFIRDVLEKAVKDLSIGVKIAAVRALKGQHLGELERVLKERTKLTDAE
                     RLELLSVLNDNRYDIPVRTLIALADQTREMTVVKEILRSCAIPNSDKLYPFLMRYLDQ
                     RNAKANTEMIRHLGSVGTAETVEKLYLLGKKSINPFVRRAVQDAIATIQARLGDIERG
                     WLSTAVPAETEGALSIPDGPAAGVLSVAKESKPVRAARKTEKNV"
     gene            12243..13769
                     /locus_tag="PKJ16_05205"
     CDS             12243..13769
                     /locus_tag="PKJ16_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46420.1"
                     /translation="MDCMPLPSVLIIEDDPSVSREVRQRLEARGYPVIGQAATLPDAA
                     AHIDRRRPDICLTDITNGRGTVSIDIAHAVRHEHRVPVVFMTGAEPNPHLDQARAARP
                     SSFIRFPITDHELVMAIDLAIEHHAIEMRYRESERLYRHLVEGTVDIILQTDVRGRFI
                     YINPAAEKLTGYSLDEIIGVYYLKFVHKDYRGRIKEILESLFASGKPNDYVEFPLVTK
                     HGSTIWLGQTVSLLMDHGAITGFQAVSRDITKQKELETVKARYQKRIRYEISSLRELQ
                     AAQQPRLPRFPEYDLATLFLPVEDISGDFFDAFALTPERYQIIVCDVSGHGITSSYFG
                     AEIRTLFRMMSSPNRTPAELIRLVNRSVIEHFRALDYFATVAVCQIDLPSGAMLYSSG
                     GHPESLIFRAADKTCTTIGGTGPLIGIPTPRDHSDIELRLEAGDCLLMYTDGITEMAD
                     PSGALYGVDALTNELVYNARYSAQDIIFSIISKLYEFNGYNNPGDDVTAVCVKKTPRT
                     "
     gene            13766..14251
                     /locus_tag="PKJ16_05210"
     CDS             13766..14251
                     /locus_tag="PKJ16_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV46421.1"
                     /translation="MIEIVPLARLPHLAEVLGGWAYGEWYAHRDVPRAAVIESYRHRA
                     HSRNELPIAWVALEDGRPAGMASLKPDDLWSRRDLGPWLASLYVDPHRRNRGVATRLV
                     ETVVAAARDLSFPRIFLFRSHEWLDDFYRNRGWSLYGDCVDNDGRPAKIYLMQLAPPT
                     V"
     gene            14326..14703
                     /locus_tag="PKJ16_05215"
     CDS             14326..14703
                     /locus_tag="PKJ16_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46422.1"
                     /translation="MASKPYIDFKLIGVRPFTAALDDSKVPYVLFEGDGDALFDTHGE
                     LFNRETDTIRVGRCTGAGTDAYFAEFGIRITKSFNSHIVFIFDHHPTGGDLAETADAI
                     EPLIGEGLAAVNPGDLAPKDALH"
     gene            complement(14873..15298)
                     /locus_tag="PKJ16_05220"
     CDS             complement(14873..15298)
                     /locus_tag="PKJ16_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46423.1"
                     /translation="MDDSTLSRFRGICTAVCAALPTGSEWQWDDRFEVGLASFDASSR
                     EAMLAALRERFPLAIEAQTLKAAPKDIQKIIKDVFDLKAGQLVFAAEQESDAMLLAAW
                     WPWGDGQTTSLRIGVYPRGGKPFDAETAHTSLAALLPLS"
     gene            complement(15342..17003)
                     /locus_tag="PKJ16_05225"
     CDS             complement(15342..17003)
                     /locus_tag="PKJ16_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46424.1"
                     /translation="MHRALIALAALVAMREAAAMPPPQYLAPAKLSVTVQGEHRIDAV
                     DYEEEARAYYRGKLIMRFSRTSALYGAYVAVEERNGPDRTGEDDSRRSYTWSLAVRDA
                     SPYVALYVGNYYVNFGSGLLIGGWMPSNPDVFFSRDAIPRRETFAPCTSGNPLYAYRG
                     GALALRTPGEGDVIATLHPFYSSKERYLDPKTADSGATNSSIVSMDYKYEQREAWSRP
                     VELVTWGAAAALHIIESFLLQAHYVNTGVSFYSGDALEWDRDAPGNDDRGIVSLDGYG
                     ALAEYRDRFVRIFVEHDRTRRLLRGDDGARGETCGAGSVGGLQFRHPLVSLSIIGKST
                     DADYYAPFQSSVGARSPEEAWFVNAAVELARGLALGGAVSSQRPHLLAPGERECAAVI
                     RERVSLRYIPSDRLSVELAGRRYEGRERGEEREREQAQGNALAWPWESCGVSARALFQ
                     RQDGREWSDLLGGGIAVRWRRRVECALEYAKARIAGENRLYAVVSPLGGANIPGIFIE
                     ETSHVAAARLRITWPPVTLAARYLYQFTDEAPLRTRMEFFASVRL"
     gene            complement(16988..18295)
                     /locus_tag="PKJ16_05230"
     CDS             complement(16988..18295)
                     /locus_tag="PKJ16_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46425.1"
                     /translation="MSAIAVVLIIGRLATHAFEFRESNPAALFPYYCAVDDTGSGNLA
                     NPSFLPLNRYAYICSSYNRPYADLEVQAANARIGQGLERFGWQVAWGGFGIDAYREDT
                     VEANVGWRPVRMLSVGAGASYYSLSINTEDLGHAIGMYDGRASVLFMPVAWADIGVRQ
                     ENIGSLFMREREDLLFPETSAGVALKPARGVALIYNFNRTYYGALNSVAVSANLLDCL
                     NVKVGYSRETTTYAASISIAVSVVRASYGIRQHPYLGLSHSFAVTVATGDIPFEQIPY
                     TEPVMTRREAIPASQKLDINNCVIEELRELPLMTDALAARIMQHRRMIGPVNARILEQ
                     LGMSRRDIAAFRSYARWDDPKKKREWSPRSPKRVPSRANAHERTKALFRQLLESGLTA
                     ADALDLSELAGSAQPAEIERRAAAIRGITESTRRLVVKLCTGR"
     gene            complement(18292..19332)
                     /locus_tag="PKJ16_05235"
     CDS             complement(18292..19332)
                     /locus_tag="PKJ16_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46426.1"
                     /translation="MKRISTMVLIVYAVAGLFQGVCVYAGQLLITEVACGTKGDDWAE
                     VFFQGAPGERIDIARLYVTMYYGANEPLSDSPITLHGADRPETPYDDRYAVIHLNGRG
                     RADETDLTGDTDRNGAIDVYCENHADSLWNTDCVVAIDTDDDPANGGIVDFVAYSNRD
                     GSVNATMAGYVAQAQIHAQWAACSEENLQQCMADVGADGLATHQSLARVPGADTNSAA
                     DFRITPYQTPGRDNVFDTGKKPGGIFEVEKGTITIIPTHPTLGTGKIAIMVHEPCALR
                     LRVFNAIGMLVHESPLYRDVNPGPFSFTWDLTGRGRPVCTGLYFGHIEGSHYASRRSV
                     SRTVYIVVSRYR"
     gene            19516..>20355
                     /locus_tag="PKJ16_05240"
     CDS             19516..>20355
                     /locus_tag="PKJ16_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46427.1"
                     /translation="MNDMPPYSQKRFNLRFLPFLMLAVYTVCIACNQQDIFEALTSKN
                     ETAAKTLHWQFVDGTPTTAVGLNHISSYDAQDVHLLVFNNQLYAAWAERNGNAGNPYQ
                     IRVARYNGDRTWALIDGSGANGLNANASLNAFSPHLGIYAGSLYIAWNETNGTEARIR
                     VKRYDGGAIWTFVDGGCVGDGINYAANGIGYNAYSPRIAEFGSFMMVTWHETNGTADQ
                     VRVRYYNGANWLWADPGGTTGINFDTGVIGRNPQSVIFNSSLYCTWYEYDGGVGQIRI
                     RSYN"
BASE COUNT         3978 a         6605 c         6143 g         3629 t
ORIGIN      
        1 tcgcggaatc cctgctcgac gtgtatctgt cgcaggacaa gcaggccccg aaagagctcc
       61 gggagctcga gcgcgccctc gagggctaca acatcatcct ccgcttcacg cgcgacgtct
      121 cctcggcgaa caagcggctc atcctcgact actgcattta cggccgcaag aagctcatca
      181 agatctcgca cccgctgttc aatatgcgcg aaaaggtgta caacatccag ccgctcatct
      241 actacgacga gttctcgacc agcaactcga ccttctactt cgacatgatc tacatcaacc
      301 cggaagaggt gcacaacgat tacgtgatcg cgaaaaacgt cctggcggga aagagcgtga
      361 agtccatgtt cttcgtgggg gcgcgcgtct ccgaggacat cgcgagctgt ctccggcgcg
      421 cgtttgcggg gggcaggccc atcaagcccg agatcctgcg catgttcgag atccacgagc
      481 tcacgcacaa gatcctcaac aaccattaca acttctacga ccaggtgatc ggcgaggagc
      541 tggcgctgtc gagcaccatc tacgccaatc ccttcctggg gctctcggtg ctctacagct
      601 atctcgacta cagcgtcatc aacccgcacc gcatcgcggc gatgaacttc gtgcggttcg
      661 ccgcgagccg gacgcgcaac accgacatga tcgacaatcc ctcgctcatg cgcgcactcg
      721 gcgaggagga gatcctcaag ctcgcgcgcg agcacttcaa ctcaatcatc aagaccctcc
      781 ggcgctccaa ctagacccgc agccgcaccg gttccttgtc cttcatggcc agatagtcct
      841 ttccgtcctc gccgctgagg ttgttcgtgc gcaggtcgcg gataacgtcg ctgtaattgt
      901 cgttcccctg gcgcagcttc gcctcgaccc gcgcccagag gtcgacggtc cggtccacga
      961 ccacgcgcat gaggtcctcg accgactcgt agtggatgcc ccgcgcgccc gcgggataca
     1021 cccagcgctg gcgatgcagg ttgaggacat ggctgttgat ccgtcgggga tcaggatagc
     1081 gcaccaggta atcgttaaag agcgcggtgc ggcggcgcag cgcgtcgagg agcgcattga
     1141 gccgcgggtt ccggttgcgc ttgatccagt aggtcgcgcg gatgagcgag ggaagcgcgt
     1201 ccagaagcgt aaacgcgttg gcgtcgccat cgacatcgcc gctcgggcgt ccgagaaaga
     1261 ggcgccggta ctgatcggga aacccctctt tgaacgcttc gagaatgagg cgcttgaccg
     1321 gacgattgat gcgaagcccc cggcgcccct cccggagcgg cagcatatcg tccaggttga
     1381 aggcatagcc gtccgtgcga tactggtgca ggaagaacag gtccagattg tattcgaaga
     1441 cgaggttctg ctcgcggtag tggttgatct cgcggcggat gaaggcgtcg gggaagcccg
     1501 accagtaaaa catgaaggga tgatacacga tgtccgccac cgcgtgcgcg atgaatccgt
     1561 gcagataggc gcgctggacc gacgcccact ccgtgttgag atcgtccagc gcgccgatgc
     1621 ggcgcagcat cgcggcgatg aaggtgacgc tcccgccgtt gtgcatcagg tacgagagcg
     1681 gcgagccgta caattgccgc gtgagaccgg gcgcgtagtc gaagatgttc ggcccgatgg
     1741 ccccgaagaa ggccgcgttc ttgaaggccg gcgtattgag aaggatctcg acagagcgca
     1801 acgggggcgg aaggtgccgc tcacgcgcga ggtgcgcgac cgtctcgacc aggaggcggt
     1861 gatgggagat tatgccgggc attcccctag aggtgacagc ccttcccggt gcaggccggg
     1921 gtccggtaga gccgcttcac gtcctggccc ttcgcctgct tgagcccggt tgccgcatta
     1981 tggcagtcgg cgcatgattt gacgacctcg acgccggcgc gcttgaggcc ccggtgaagg
     2041 ttcatgatgg ccgggctcga gtacagcgcc gtgccgacga aaacgagcgc aatgcccgct
     2101 aggatcgctt ttcgcgtcat gggaaaacct ccgcgtgctg atccgctccg caacgcggag
     2161 cggcggtgac cggccgagtg cgccgccgga acgacggcag cagtgtgccc gccggaaaca
     2221 ggcggtcaag gatttttcgg acggaccggg gccgatgccc cggcccgatt ctccttgaca
     2281 gggacacggc gccgtgatgg aatgagcggc ggcgcgtaaa ccgtttcgtt tcggcgccgc
     2341 atcaaaccac caattcgttt cgcttttccg acaggaatcc ggccgcacct tgcgggttcc
     2401 tgccggaacc gattttacgg aaaccccgtt ttttcgggca aaatcgcgtt ttttttatcc
     2461 cgcgcgcatc tagttattga cggaaacgga cgccggtggc aagtgtgcct ctattctata
     2521 caccacccga ggaattgcaa tggcaaacgc gaaatcatcg aacaacaaga cgaaagtggc
     2581 ctacccggaa aagtacaccg tgaacaccat ctcggattat ctcgccaaga aacacggcat
     2641 cccgaagcgg caggcgaagg agatgctcga ggacatgttc gccgtaatca acagcggcgt
     2701 catgagcggc gagcgcgttc cgatcggcaa gatgggcaag atgttcatcc gcgtccgccc
     2761 ggcgaccaag gcccgccagg gacgcaatcc gctcaccggc gaagagatca ccatccccgc
     2821 aaagaaggcg accaaggtcc ccaagttcgc gttcaccaag accttcaaga ccgaggcgac
     2881 gaaggcgaag gtcaagacga agtaggcgtt tccattcgat gcgcaatcag gaaggcgggt
     2941 gagagatcac ccgccgtttt attgtccgca caccggggtt tccgggatgt ttcgtctcag
     3001 ggtgcgcttt ttcccggccc acgcaccggg aaaaacgact gttcgatgtg cgcgcttcca
     3061 ttccccgcca aatttttccg cgacaccgcc attcattttt tcatgacgat aaagacgatt
     3121 accccgataa tacacaggag tataacagcg aacgccgcca actgggtata gatattcccc
     3181 tcgtcggccc cgtccgccgc accagcggcc tttccgtcat cgtcattcac cggctctttc
     3241 ttccgctgct gctcgatgta cttatcgaca tatttctcta tccccttctg gagctcgggg
     3301 gggcggtcgg gagacggagc gacgaaagcg accttgatgt tgcgctcatc ctcctcgatg
     3361 cggacataga tcccgtccgc gatttcgacg aatatcttgt agccgccggt cgcctttttc
     3421 cggtacgaca cgatgctgcc gacgatcggc atcatctggc cgtcggcata ggcattgaac
     3481 gccttggcga tgagcaccgc cttccggttc tcgtcgacga gacggatttt tatcgaatcc
     3541 ttcacgacca ggccggcgac gttcatgccg ttgatgggat cgagcaccag cgcgccgtgg
     3601 aagatgagct tgatcttgtt tttatcaaac gtgtcctcat cgtccttttc gatgacgatc
     3661 ggcgcatcgt cggccatgga cccgtccgct gccggcggct gcgcgccgct gatttgctcg
     3721 ggcgtcagct tgatgctgct tttcgagtag cgctccatgt cgagcgatga aatcgtctcg
     3781 aagtgaacat ccagcttgat atttcccagc tcgatgccgc tgaacacctg ccgcttgaat
     3841 atgctcgcca tgccgggcag gtcctgcatt tcgacaaaat actgcagatc cttggcaaat
     3901 gtcgcggtaa cgccgtttcg gactgcctcc agaaacgaaa agacttggcc cttgtcgtgt
     3961 tcgcaggtgt taaaatactc ggatatctcg cgctcgaact ccttccagtc cttgtgtgtg
     4021 gaaatatcgc ctacgctata cgtcggcgag atgatgccat agcaatcggc gagctggttg
     4081 tatattctat taaagaagag cagacacgcg ccattgatca tcggagaggt gaatttgatc
     4141 ttgaacacct gcaggtcatt atacgatccg gcgaccatgt tgttcgcgct ctccacgtcg
     4201 cctttcgtgt aatacagggc gatcttcagc gccgtggctt catcaatact atgatcgcgt
     4261 cgtggttgtt cgattggggt attcatacta tgctgttcat cgatgctaat gtatcttggt
     4321 attctcgaat cattctcgtc agggcggtac accaatccgg cttttcggta gagtcaatca
     4381 taaataatgt cccagatcgg cgagaattaa atggtgcctt cgatacgccg actgcgtatc
     4441 accattcagg gacccggtcc ccttccgagc aaaaagtgcc tgacgccatg aatttttcgc
     4501 ctttttatga ttgacaaaaa gagtctcctt gtgttattgg tcgaaccact gagccattga
     4561 accaataatg ccatgacagc acagcccgca catctggacc ccgtttcgaa aaaccggctg
     4621 cacgacgaga tcgtgcgcca gttccagcgg aagatcgtcc gcggcgagtt cgccgtgggg
     4681 cagaagctcc cgcccgagcg cgagctcgcc gcgtccctcg cggtgaaccg cgcgaccctg
     4741 cgggaggcgc tcaagaaact cgaggtgctg ggcctgctcc agatcctcca cggcgacggc
     4801 atctacgtga aggacttccg ggccagcggc aacctggagc tcttcaaggc gttggtgtac
     4861 ctggacagcg tcatcaacgc ggacatcctg cgcaacatgc tggacatacg gaagctcatc
     4921 acgccggaga tggccgcacg ggccgcgcac aaccgctcgg aggcggacct ggcgcgcctc
     4981 gcgcacatcg tcgagggcga gaaccccgcc gacatcctgg agcgcgatct tgcggtgcac
     5041 aacggcatcg cgctgtccgg cgggaacctc tgctacctgt tcatccacaa cttcttcagc
     5101 gggatcatcc gggattttgg acatctctat tttgccgatc ccgcgaacgt ggaacgttct
     5161 cggcgcttcc atcgggacat tctggaggcg atccggaaga aggacgcaaa gcgcgcgcat
     5221 cgcgtgatgc acgacgtgct cgactattcc gaaaaaagta tttacgcgca gtacgatgcg
     5281 cggtataaca cgcacagcta aggaggaagg ccgtggaacg atttctggac acctatcgcg
     5341 gggcgcgatt cgacatcatc gtggtcgggg gcgggatcac cggcgccgcc gtggcctacg
     5401 aggccgcgag ccggggactc tcggtggcgc tcctggagaa gaacgatttc ggctgggcaa
     5461 cctcggccgc gacctcgaag atgatccacg ggggcctgcg ctatctcaat aacttcgagc
     5521 tggggctggt gcgcgaatcg ctgcgggagc ggcgcatcct ggaaaacatc gcgcccaatc
     5581 tcgtgtatcc gttccccctg ctcattccca actacaagaa gctcatgacg cgaaagagcg
     5641 tgctcaattt cggcctcacg ctctacgaca tactcgcctt cgacaagaag cggacgtggg
     5701 acccgagcaa gcgcatcccc aaccactcga cctattcgcg ggacaagacc cgtgcgatcg
     5761 cgcccggcgt gaaaacggac ggtctcaccg gctcgtgcgt cttctacgac tgccgcagca
     5821 tcttccccga gcggctcacg ctggccttcg tgcgttcggc catgcgctac ggcgccaagg
     5881 ccgcgaacta cgcgaccgtg acggggttcg tgcgcggtcc cggcgggcgc atcgcgggcg
     5941 ttacggtaaa ggacaccgtg aagcgccgcg agatggagat ccacggcgag ctggtggtca
     6001 actgcggcgg tccgtgggcc gacatcgtgc tcaatacggc gaagggcggc gcgaatgatc
     6061 accccatgaa acgctcggag gggatccata tcatcacaaa aaagctgtac aatgagcacg
     6121 ccgtaacgct gcaaaccccg gagggacggc acttcttcat cctcccctgg cgcaatcacg
     6181 cgctcatcgg caccaccgac aaggagttct tcggatcgcc cgacgactac cgcgtgtcgc
     6241 ggcagagcgt ccaggagctc atcgacgaag tgaaccagtc ctacggggac ggcacgctct
     6301 cctacgacga tgtgctgttc gcctacggcg gcctgcgccc gctcgtggag cagcagacgg
     6361 cggagaccta cgagtcgtcg cgcaagtacg agatccacga caacgccgag gagggcttcg
     6421 aggggcttat caccgtggag ggcggcaagt acaccaccag ccgcaacctc gccgacaacg
     6481 tgctcaagat ggtgaagatc aagctgcatc gcagcctcac gacgtcgatc accgaccgga
     6541 tttatcttca cggctgcgag atcaaggacc tggaggagtt cgtccgccag gtgcacaccg
     6601 cccacgccga ctttccgaag aacacgcgcg aatacctggc gcgaaactac gggaccgagt
     6661 gccacgcggt cttcgacatc gcccgcgaga acacggcgct tgcgcagccc gtgaacgggg
     6721 acggcgagat cctcgcccag gtggcctacg ccgcgcgccg ggagatggcg ctcacgttaa
     6781 acgacattct ttttcgacgg accggactgg gcacgctggg cgatcccggc gcgaaggtcc
     6841 tgaacgcggc ggcgaaggtc gcggcgaagg agctccgctg gaacgccgcg cgcgtcaaga
     6901 aggagatcgc cgccgcaaag aaggcgctcg cgctgcccgc ataacacgag acacgaacaa
     6961 tacaaccgga ggctaccatg tacggcaatt atcgacacat cttcaaatgg ggcgatccct
     7021 cgcacgagga gcagctcaag gaccacgtgg tccattttgc gaaggagcat ttcgggatga
     7081 cggacgccga cttcgcgaac aagcacctcc ccggcttcga caccatcacg ctaaaaaaga
     7141 ggccgcggct gtcgaaggcg tcgctggggg ccctgaaaaa gatcgtgggc gcggaaaacg
     7201 tgagcaccga ggacttcgag cgcgcctatc attcggcggg aaagttctac ctcgagctcc
     7261 taaagctgcg gctgggcgtc atcgacaatc cccccgacgc ggtggtgtac ccgcgcgacg
     7321 agaaggacgt gatcgcgatc gtgcgcctgt gcaatgcgaa gcggatcccc atcaccccct
     7381 tcggcgggca ttcgtcggtc acgcgcggcg tggagcccgt caagggcggg atctcgcttg
     7441 acctcacgcg ccacatgaac gcggtgatcc gcgtgaacga gatcaactcc accgtgacgg
     7501 tgcagcccgg catgttcggg cccgccctgg aagaattcct caaccgctac aaagcgggct
     7561 acacctgctg gcacttcccg caatccttcg agttctccac cgtgggcggc tgggcggtca
     7621 cgcgcggcgc gggacaggcc tcgaccggct acggcaagat cgaggacatg gtgctgtcca
     7681 tgcgcacggt cacgcccgcg gggatcatcg agacgaagga ctacccggcc gcggcgatcg
     7741 gtcccgacat cgaccagatc atcatcggct cggagggcgc ttacggcgtg gtgaccgaga
     7801 tcacgatgaa gatccgcacg taccggccgc agaacacgtc ccacacctcg ttcgtcttca
     7861 aggacttcaa gagcgccgtg accgccatgc gcacggtgat gcaggccgag ttcggaaagc
     7921 cccacctctt ccgcatctcc gaccccgagg agaccgagat ggccttccgg atgggcggca
     7981 agcacggggg gctgggcgac aaggtcctgt cgctgttggg atacaagccg atgcagcgct
     8041 gtctcatgtt cgtttcggtc gagggcgacg cgaactacac gaagttcgtg gtgggcaaga
     8101 tcaaggacgt ggcgagcgat ttcggcgcgc tgtgcaccgg cgcgggcccc acgaagaagt
     8161 ggctggagca gcgctactcg agcgcgtacc tgcgcgatcc gctcatggac gcggggatcc
     8221 tcgccgacac gctggagacg tccgtgtcgt gggagaacct caaccagctc tggagcgagg
     8281 tgcgcgacta catcaaggcg cgccccaaga cggtgtgcat ggtgcacctc tcgcatattt
     8341 acgagaacgg cgcgaacctc tacttcacct tcctcacgcc catgaacaag ggcgacgagg
     8401 tgcgcgattt cgtccagttc cacaagggca tcgtcgacac cgtgcacgcg aaccacggct
     8461 cgatatcgca ccaccacggc gtgggcaaga tgctcgcgcc ctggatgccc aaggagcacg
     8521 acacggcgtc catcgagctc atgcgcgcca tcaaaaaaca cctggacccg aaaggcatca
     8581 tgaatccggg gacgctgggc ttgtagcagg gacgattttc gcggcagtca gcgtactggg
     8641 gccgggtcga ttcgatccgg ccctttgttt tttcagggcc gcggattttt gttgcgcgaa
     8701 aaattccggc ccgtatactg cttcaactgg ccgcgttcgc gcactcatcc gagcattccg
     8761 atcaataccg cggcccggcc atcaccgtcg aggaggaatc atcatgaacg gatatcggac
     8821 cgcagccatc atctgccttc tgattatcat accattgagc attcaagccg agagcgacga
     8881 tgcgcggaag ctcagaaacg ccatggaaac gctcgcgcag atcgcggcca tccccgaggg
     8941 cggcatcccg accgcgctac tggccaatgc ccgcgggatc gccgtcctgc cgggcacggt
     9001 caaggccggc tgcgtgctcg gcggacggta cggcacgggc gtgttgtcga tacagaacgc
     9061 ggacgggacc tggagcctgc cggccttcat caccctggcg ggcggcagcc tcggctggca
     9121 gatcggcgcg caggggaccg acatcattct ggtgttcaag agccgcaagg gaatcgacgc
     9181 gatcgggaac gggaagctca cgctgggggc cgatatcgcc atcgccgcag gccccgtcgg
     9241 gcggcaggct tcggccgcca cggacatcga gctcaaggcg gagatatact cgtactcgcg
     9301 cgcccggggg ctcttcgccg gcctcgcgct ggagggggcg ctgctcaggc tggacgagaa
     9361 ggcgaaccac gcctggtacg ggaccggttc gaacccggaa tccatcctca ccgccgcgcc
     9421 gccccgtgtc cccgaggagg cgaaaggctt ctcggcggag ctgaaaaaac tcatggtgcc
     9481 ccggcgctga tggcacgcac caccgcgctc gcaacggccg cggcgccgcg catcctggtg
     9541 tcgcataacg gagtatcgca atggacgtga cgcttgtcat cgtgctcgcc ctcggggcca
     9601 tctggatggc gtatgccgtt aaaatgtcgc ggcgcatcta cagccacacc gatctcatga
     9661 tgaagcagac ggacgaactc gtcatcgtcc ccgcctccgg cgcggcgccc gaggtcgtca
     9721 tacatctggt caacggatgg aacgtgacgg gtgaatataa cgcggacggc gaggcgaacc
     9781 tcgtctccat ccggatcgag gcgccgaagc cgtgcgcgac gtccttcacc atctcccggg
     9841 aaacggccgc gtcgcgcttc aagaaaaacc tgggcatgga cgatatcgcc gtcggcgacg
     9901 accgcttcga cgacgaatgc ctgtgcaaat cgaacaacac gctgttcctc ctcagccgcc
     9961 tggggtccgc ccttcgctcg cgcctgctgg cctttgccga ccgcgccgac gagttcacga
    10021 tcacgcacgc cgggtttact gcgcgcttca gcgccgtgac gggggagcga ccggccttca
    10081 tcgatcggac ggccgaggaa atgatcgcta tccaccgcgc catcaccggc tccccggcga
    10141 ccaagccgcg cctcaagagc atcatcttcg acgatccgat cgcgcacgtc cgcctgcgct
    10201 gcctggaggc ctatgcggcg cagttcgggc tcgacgcctc attcgagcgc tgcctcgcgg
    10261 atgcggacat cggcgtgcag atcgccgcgg cgcggcacct gggcgagaag gggctttcgc
    10321 acctggccac actcatcggc tcgcaacccg acatcgcgga cgaggacaaa acggagatca
    10381 ttcgtttttg taacgaacga acacatccgc ttccgggaac gatcctcgcg acattgctcg
    10441 acggtgcgca tcaccgcgat cttctcagtg aagcgatcgg ggcctgtcgc ttttctagcg
    10501 atccgtccct gtcggagcac ctgctgcgcc ggctcgaacg cgccggcctc gacgatctcc
    10561 tccgcctcga gattatcctc gtacttggcg attgcgggac gctcgcggcc gtggagccgc
    10621 tctaccggat cgcggagaac cgccggctct catcgggtcc gcgcactgcc gcgaacgagg
    10681 cgatcgcgag gatccagtcg cgtcagggca cgggcgagcg cgggtggctg tcggtatcga
    10741 cgcccgcggg aaaggagggc gctctgagca tcacgcgcgg cgccggccag ggtgcgctga
    10801 gttcccccaa cgctgcccct acggtaaaat caaccgctcg tttgaagacc ggaaacaggc
    10861 ggtcatcaaa aaaaacgtca aagaaaaaaa agtgacgcca tgacctggtt cctcgtcatc
    10921 gtcggcggac tcgtcgcgtt tcttctcatc cagcttgtca tgcgatggct ggattccccg
    10981 ccaatgctga ccgtggcaga cgagccgttc aagtcgctcc tctcggacgt ccgtaccgac
    11041 aacggcgcga tgcgcggaac ctatcgtggg catcctgcga ttgtttcctt tacatacgaa
    11101 aaaagcaggt cctacctgtt tgaagtcatc accaccatcg tacgcatgga catccctccg
    11161 cacatcaccg gcccgttttc cgtctccagg gagacggtcc tctcgaactc gaaacgtttg
    11221 atcggcaagg gcgattatca gacgggcgat gtccgcttcg acagaacgtt cctcatctca
    11281 tcggaccatc cggttgtcct tccggcgctg ctgaacgctg cggtccggaa aaatctcctg
    11341 aagctcaact cgtccgccta cgcactcagc ctcagcaacg agcagttcat ggccgagctg
    11401 ccgcgcccct caaagttcgg gaaggcaatt cgcaacgcgt tcgaaaccat ggcgtcgatt
    11461 atcgacgata tcgcgtcccc ggcgtcgatc cgcgatcgcc tcgtcagcag cattctgcac
    11521 gaccccgtgg cggcggtccg ccgaaactgt ctggaagcgc tcatcgccaa cgtaccgatc
    11581 gacacgttta tacgcgacgt gctggaaaag gccgtcaagg acctgagcat cggtgtgaag
    11641 atcgcggccg tccgggcgct gaagggccag cacctgggcg agctcgagcg cgtgctcaag
    11701 gagcgcacaa aactcaccga tgccgagcgc ctcgaactgc tctccgtcct caacgataat
    11761 cgatacgaca tccccgttcg aacgctcatc gcccttgccg atcaaacacg cgagatgacc
    11821 gtagtgaagg agatcctgcg gtcatgcgcc attccgaatt cggacaagct ctatccgttc
    11881 ctcatgcgct atcttgacca acgaaacgcg aaggcaaaca cggaaatgat ccgtcatctc
    11941 ggaagcgtgg gaacggccga gacggtcgag aagctgtatc tgctcggaaa gaaatcgatc
    12001 aatccgttcg ttcgccgcgc cgtacaagac gccatcgcca ctattcaggc gcggctgggt
    12061 gatatcgagc gcggctggct ctccacggcc gtaccagcgg aaaccgaggg cgctctcagc
    12121 atacccgacg ggcccgccgc gggcgtcctt agtgtcgcta aagaatccaa acccgtccga
    12181 gcggctcgaa aaaccgaaaa aaacgtttga actaagccgt cggtttcgct accgtgtaac
    12241 acatggactg catgccgctc ccttcagtgc tcatcatcga ggatgatccg tccgtgtcgc
    12301 gcgaggtgcg ccagcggctc gaggcgcgcg gctatcccgt gatcggacag gcggcgaccc
    12361 tgccggacgc cgcggcgcac atcgaccgcc ggcggcccga catctgcctc accgacatca
    12421 ccaacggccg gggaacggtc agcatcgata tcgcgcacgc cgtgcgccac gagcaccgcg
    12481 tgccggtcgt cttcatgacc ggcgcggagc ccaatccgca cctcgaccag gcccgcgcgg
    12541 cgcgcccatc gtcattcatc cgcttcccca taaccgacca cgagctcgtg atggccatcg
    12601 acctggcgat cgagcaccat gccatcgaga tgcgctatcg cgagagcgag cggttgtacc
    12661 gccacctcgt cgaggggacc gtagacatca tcctccagac cgacgtcagg ggacgcttca
    12721 tttatatcaa ccccgccgcg gagaagctca ccgggtacag cctggacgag atcatcggcg
    12781 tttactacct gaagttcgtc cacaaggact atcggggccg catcaaggaa atccttgaat
    12841 cgctatttgc gagcggcaag cccaatgact acgtcgagtt cccgctggtc acgaaacacg
    12901 gctcgaccat ctggctcggg cagacggtga gccttctcat ggaccacggc gcgatcaccg
    12961 gcttccaggc ggtatcgcgc gacatcacca aacagaagga gctcgagacc gtcaaggcgc
    13021 gctaccagaa gcgcatccga tacgagatct cgtccctgcg cgaactgcag gcggcgcagc
    13081 agccgcgcct tccgcgcttc ccggagtacg acctggcgac cctcttcctt ccggtggagg
    13141 acatctcggg cgatttcttc gacgccttcg ccctcacccc ggagcgctac cagatcatcg
    13201 tctgcgacgt gtccggacac ggcatcacct cgtcgtattt cggcgcggag atacggacgc
    13261 tcttccggat gatgtcgtcg ccgaaccgca cgccggccga gctcatccgg ctcgtgaacc
    13321 ggtccgtcat cgagcacttc cgcgcgctcg actacttcgc cacggtggcc gtttgccaga
    13381 tcgacctgcc ctcgggcgcg atgctctact cctcgggcgg tcaccccgaa tcgctcatct
    13441 tccgcgccgc cgacaagacc tgcacgacca tcggcgggac cggcccgctc atcggtatcc
    13501 ccacgccgcg cgaccactcg gacatcgagc tgcgcctgga ggccggcgac tgcctgctca
    13561 tgtacaccga cggcatcacc gagatggccg atccctccgg cgcgctctac ggcgtggacg
    13621 ccctcacgaa cgagctcgtc tacaacgccc gctacagcgc gcaggacatc atcttttcca
    13681 tcatctccaa actctacgaa ttcaacggct acaataatcc gggcgacgac gtcacggccg
    13741 tgtgcgtgaa aaagaccccg cgtacatgat cgagatcgtc ccgctcgcgc gcctgcccca
    13801 tctggccgag gtcctgggcg gctgggccta tggcgaatgg tatgcccacc gcgacgtgcc
    13861 gcgcgcggcg gtgatcgaat cctaccgcca ccgggctcat tcacgcaatg agttgcccat
    13921 cgcctgggtg gccctcgagg acggccgtcc cgccggcatg gcgagcctca agccggacga
    13981 cctctggtcg cgacgggacc tcgggccctg gcttgcctca ttgtatgtcg atccgcacag
    14041 gcgcaatcga ggcgtcgcga cgcggctcgt ggaaacggtc gtggcggcgg cccgcgatct
    14101 gagttttccg cgcatcttcc tgttccggtc ccatgaatgg ctggacgatt tttaccggaa
    14161 ccggggatgg tcattatacg gcgattgcgt ggacaatgac gggcgcccgg cgaagattta
    14221 cctgatgcag cttgcgccgc ctaccgtttg atcgcgcccg cgtttttcag tttacagaag
    14281 gcggccgatc cggttgcctg tcgccagcgc agggaccgaa accgcatggc atccaaaccc
    14341 tacatcgact tcaagctcat cggcgtgagg ccctttacgg cggcgctcga cgattcgaag
    14401 gtgccctacg tgctcttcga gggcgacggc gacgcgctct tcgacacgca cggcgagctc
    14461 ttcaatcgcg agaccgacac catccgcgtg ggacgctgca cgggcgccgg gacggacgcc
    14521 tacttcgccg agttcggcat ccggatcacg aagagcttca actcgcacat cgtcttcatc
    14581 ttcgatcatc atcccaccgg cggggacctc gccgagaccg ccgacgcgat cgagccgctc
    14641 atcggcgagg ggctcgccgc cgtcaacccg ggcgacctcg cgcccaagga cgcgctgcac
    14701 tgatccgtcc ggaacgcgtt cgcaacaccg gacatacccc caccctgcct ccccctttac
    14761 acaggggaag tgtccgtaga acggaggggg tcatggctat cgtaggccgc tgaaaacggg
    14821 gtagtgctac tcaatgcggg atgatgccgc gcggcacaca tcgcccgtcc ggtcaggaca
    14881 ggggcagcag ggcggcaagc gacgtgtgcg cggtctcggc gtcgaagggt ttcccgccgc
    14941 gcggatatac gccaatccgc agcgaggtgg tctgcccgtc gccccagggc caccatgccg
    15001 ccaggagcat cgcatccgat tcctgctccg cggcgaacac cagctgcccg gccttgagat
    15061 caaagacgtc cttgatgatc ttctggatgt ccttgggtgc ggccttgagc gtctgcgcct
    15121 cgatggcgag gggaaagcgc tccctgaggg cggcgagcat ggcctcgcgc gaagaggcgt
    15181 cgaacgacgc aagccccacc tcgaaccggt cgtcccattg ccattcggag ccagtgggaa
    15241 gcgccgcgca gaccgccgtg cagatgccgc gaaaccgtga gagtgtactg tcgtccatgc
    15301 gttaatctcg cacgaagcgg ccgatcgcgt caaccacaat atcacagccg cacgctcgcg
    15361 aaaaactcca tgcgcgtccg cagcggggcc tcgtcggtga actggtagag atagcgcgcg
    15421 gcgagcgtta ccggcggcca ggtgatccgc aagcgcgcgg cggccacgtg cgaggtctcc
    15481 tcgatgaaga tgccggggat gttcgccccg cccagcggcg aaacgaccgc gtagagccgg
    15541 ttctctccgg cgatgcgcgc cttcgcgtat tccagcgcgc actcgacgcg cctgcgccat
    15601 cgcaccgcaa tgccgccgcc cagaagatcg gaccactcgc gcccgtcctg ccgttggaac
    15661 aatgcccgcg ccgatacgcc acacgattcc cacggccatg cgagcgcgtt cccctgcgcc
    15721 tgctcccgtt cgcgctcctc gccgcgctcg cgcccctcgt agcgccgccc ggcaagctcg
    15781 acggacaggc ggtcgcttgg aatatatcga agcgaaacgc gctcgcggat caccgccgcg
    15841 cattcccgct cccccggggc cagaagatgt ggccgctgcg aggaaacggc cccgccgagc
    15901 gcgagcccgc gcgcgagctc caccgcggcg ttcacgaacc acgcctcctc gggggagcgc
    15961 gcccccacgg acgattgaaa cggggcgtaa tagtcggcgt cggtcgattt cccgatgatc
    16021 gaaagcgaca ccagcggatg acggaactgc agcccgccga cggagcccgc gccgcaggtc
    16081 tcgccgcgcg cgccgtcgtc cccgcgcagg aggcgccgcg tccggtcgtg ttcgacaaag
    16141 atgcgcacga accggtcgcg gtattccgcg agcgcgccat agccgtcgag tgagacgatg
    16201 ccccggtcgt cgttgcccgg cgcgtcgcgg tcccattcca gcgcatcgcc cgagtaaaac
    16261 gacacccccg tattcacata gtgcgcctgg agcaaaaagg attcgatgat gtgaagcgcc
    16321 gccgcggcgc cccaggtaac aagctccacc gggcgcgacc atgcctcccg ctgttcgtac
    16381 ttgtagtcca tggaaacgat gctggagttt gtcgcaccgc tgtccgcggt tttcggatcg
    16441 aggtagcgct ccttcgacga ataaaacggg tgcagcgttg cgatcacatc gccctccccc
    16501 ggcgtacgaa gcgcgagggc cccgccccga taggcgtaca gcgggttccc gctggtgcac
    16561 ggagcgaagg tctcccggcg cggaatcgcg tcgcgggaga aaaagacgtc ggggttggag
    16621 ggcatccatc cgccaatgag caggccgctt ccgaaattca cgtaataatt cccgacatac
    16681 agcgcgacgt acggcgaggc atcccgcacc gccaagctcc aggtgtagct gcgccggctg
    16741 tcgtcttcgc cggtgcggtc cgggccgttt cgctcctcga cggcaacata ggcgccgtag
    16801 agcgcgcttg tccggctaaa gcgcatgatg agcttgccgc gatagtaggc gcgcgcctcc
    16861 tcctcgtagt cgaccgcgtc gatgcggtgc tcgccctgca ccgtcaccga cagcttcgcg
    16921 ggcgcgagat actgcggcgg cggcatcgcc gccgcctcgc gcatcgccac aagcgccgcg
    16981 agcgcgatca gcgcccggtg catagcttca cgacgagccg tcgcgtggat tcggtgatgc
    17041 cgcggatcgc cgcggcccgc cgctcgatct ccgccggttg cgccgagccg gcgagctcgc
    17101 tcaggtcgag cgcgtcggcc gccgtgagtc ccgactccag gagctgccga aaaagcgctt
    17161 tcgtgcgctc gtgcgcattc gcgcgcgacg ggacccgctt cggcgaacgc ggcgaccact
    17221 cgcgcttctt tttcggatca tcccaccgcg catagctccg aaacgcggcg atgtcccggc
    17281 gcgacatgcc cagctgctcc aggatgcgcg cattcacggg accgatcatc ctgcggtgct
    17341 gcatgatccg cgcggcgagc gcgtcggtca tgagcggaag ctcccgcagc tcctcgataa
    17401 cgcagttgtt gatgtcgagc ttctgcgacg cgggaatggc ctcgcgccgc gtcatcaccg
    17461 gctcggtata gggaatctgc tcgaatggaa tatcgcccgt ggccaccgtc acggcgaagc
    17521 tgtgcgacag ccccaggtac ggatgctgac ggatgccgta cgaggccctg acgaccgaca
    17581 cggcgatcga gatcgaggcc gcgtaggtcg tggtctcgcg cgaatagccc accttgacat
    17641 tgaggcagtc cagcaggttc gccgaaaccg cgacgctgtt gagcgcgccg tagtaggtgc
    17701 ggttgaaatt gtagatgagc gcgaccccgc gcgccggctt gagcgcgacg cccgcgctcg
    17761 tctccgggaa gaggaggtcc tcgcgctcgc gcataaacag cgaaccgatg ttttcctgac
    17821 gcaccccgat gtcggcccat gcgacgggca tgaaaaggac cgaggcccgt ccgtcgtaca
    17881 tgccgatggc gtgccccagg tcctcggtgt tgatgctcag cgaatagtac gatgcgcccg
    17941 cgccgaccga aagcatccgc acggggcgcc agccgacatt cgcctcgacg gtgtcctcgc
    18001 gataggcgtc gatgccgaat ccgccccatg cgacctgcca gccgaaccgc tccagaccct
    18061 ggccgatgcg cgcgttcgcc gcctgcacct cgagatcggc gtacggccga ttgtacgacg
    18121 agcagatata ggcgtatcgg ttgagcggca gaaacgacgg gttcgccagg ttccccgatc
    18181 cggtatcgtc caccgcgcag taatacggaa agagcgcggc cggattgctc tcgcggaact
    18241 cgaaggcgtg cgttgcgagc cgcccgatga tgagcaccac ggcgatcgcg ctcatcgata
    18301 gcggctcacg acgatataca cggtcctgct caccgagcgg cggctggcgt agtggcttcc
    18361 ctcgatgtgc ccgaaataca ggcccgtgca caccgggcgg ccccgccccg tgaggtccca
    18421 ggtgaacgag aacggccccg ggttcacgtc gcggtacagc ggcgactcgt gcaccagcat
    18481 gccgatcgcg ttgaacaccc gcaatcggag cgcgcagggc tcgtgcacca taatagcaat
    18541 cttcccggtc cccagcgtcg ggtgcgtggg gatgatggtg atcgtcccct tctcaacctc
    18601 aaagatgccg ccgggctttt tcccggtgtc gaacacattg tcgcggcccg gggtctgata
    18661 gggcgtgatg cggaagtcgg ccgccgagtt ggtgtcggcg ccggggacgc gcgcgagcga
    18721 ctggtgcgtc gcaagcccgt cggcgccgac gtcggccatg cactgctgga gattctcctc
    18781 gctgcaggcg gcccactgcg cgtggatctg cgcctgcgcc acgtacccgg ccatggtcgc
    18841 gttcacgctc ccgtcgcggt tcgagtacgc gacgaaatcg acgatgccgc cgtttgccgg
    18901 atcgtcgtcg gtgtcgatgg cgacgacgca gtcggtgttc cacaggctgt cggcgtggtt
    18961 ctcgcaatag acgtcgatcg cgccgttgcg gtcggtgtcg ccggtgaggt ccgtctcgtc
    19021 ggcgcgcccg cgtccgttca ggtggatcac cgcatagcgg tcgtcgtagg gcgtctcagg
    19081 gcggtccgcg ccgtggagcg tgatcggcga atcggacagc ggctcgttgg cgccgtagta
    19141 catcgtcacg tacagccgcg caatgtcgat ccgctcgccc ggcgctccct gaaagaacac
    19201 ctccgcccag tcgtcgccct tggtcccgca ggcgacttcg gtgatgagga gctggccggc
    19261 atatacgcac acaccctgga acagtcctgc aaccgcgtac actatcagaa ccatcgtcga
    19321 tattcgcttc attgcactta tctccatgac acgggcatgt cccgccttga taatcgccat
    19381 ctgatttatt acgcattcag agcagctttt tttgattatt tatttttttc cgatacagtt
    19441 gacagacgag ggggagatgt gtaatcttac ttgtcggcca tgttatctgg aaaacaatcg
    19501 gttaaggatt tgcccatgaa cgacatgcct ccttactctc aaaaacggtt taacctccgg
    19561 ttcctcccat ttctcatgtt agcggtatac accgtctgca tcgcttgcaa tcagcaggat
    19621 atttttgaag cactgacatc caagaatgag actgcggcga aaacactcca ctggcagttc
    19681 gtcgacggca ccccgacgac cgccgtagga ctgaaccata tatcatccta cgacgcccag
    19741 gacgttcatc ttctcgtatt caacaatcag ctgtatgccg catgggccga acgaaatggt
    19801 aatgctggga atccgtacca aatccgggtg gcgaggtaca acggcgaccg gacatgggcc
    19861 ctaattgacg gaagcggcgc caacggcctc aatgccaatg cctcgttgaa tgccttctct
    19921 ccacatctgg gcatatatgc gggatcgctc tatatcgcgt ggaacgaaac gaacgggacc
    19981 gaagcgcgga tccgggtcaa acgatacgac ggcggtgcga tatggacatt cgtggacggc
    20041 ggatgcgtcg gtgacggaat caactacgcc gccaacggga tcggctacaa cgcctacagc
    20101 ccacggatcg ccgagttcgg atcgttcatg atggtgacat ggcatgaaac caacgggacc
    20161 gcagaccaag tgcgggtgcg atattacaat ggcgcgaact ggctctgggc cgatcccggg
    20221 ggaacgaccg gcatcaactt cgacaccggc gtgatcgggc gaaacccgca atctgtcatt
    20281 ttcaacagca gtctctattg cacctggtat gagtacgacg gaggcgtcgg tcagatacgg
    20341 atcagatcct acaac
//
LOCUS       DAORHF010000038        20336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1812_length_20336_cov_4.322223, whole genome shotgun sequence.
ACCESSION   DAORHF010000038 DAORHF010000000
VERSION     DAORHF010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20336
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1812_length_20336_cov_4.322223"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1482)
                     /locus_tag="PKJ16_05245"
     CDS             complement(<1..1482)
                     /locus_tag="PKJ16_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV46428.1"
                     /translation="MRTVSKNSSYLNKPWLTSYAPGVPTNINYEKKTLPEFLERATAG
                     FPKNKALTFMGFTLNYSELKGMVDRFAGALIGHGVRKGDSVAIILPNTIQCVVAYFGI
                     LKIGAIAVMNNPLYSDTELEHQLKDSEAKALVTLDLLANRMIDLRPKTHIKQIIVASI
                     GDYLPFPKSILFPLVAKKKKLAADVKQSPNVFRWKTFINNAQPMTDSPAASFDAIAQY
                     QYTGGTTGVSKGVVLTHANLSTNVQQAASWFPEMRNGAETMLGALPYFHVFGLTVSMN
                     LAVFKAWNNILVPKPQPDPLMEVLTKDKPTFVPLVPTMYIGIINHPEFERTDLSFVNG
                     CFSGSAPLPVEVIKRFEEKTGTVICEGFGMTESSPVTHINPFEKGRPKPGSIGVPVPD
                     VECRIVDIEDRATDVAPGKPGELIIRGPQVMQRYKGMPDETKETIRDGWLHTGDIAEM
                     DADGYFFIVDRLKDMVISGGYNVYPREIDEVLFTHPKVLEACAI"
     gene            complement(1600..2268)
                     /locus_tag="PKJ16_05250"
     CDS             complement(1600..2268)
                     /locus_tag="PKJ16_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV46429.1"
                     /translation="MTMRRGIFIVEDHEIFRMGLKELIDREEDLVVCGEADNASDALR
                     LIREARPAMAVIDISLKDSNGIMLIKDVNRCMKGLPMLVLSLFDEAIYAERALAAGAR
                     GYIMKHETSDSIVRAIRQILDGGTYVSAAIMDSLVQRLADRTAASASGPIETLTDREL
                     EIFTLIGKGLPTGAIARKLALNVKTVGTYRERIKEKLDIKTAADLAKRAALWVSDTSG
                     DRRA"
     gene            complement(2265..3395)
                     /locus_tag="PKJ16_05255"
     CDS             complement(2265..3395)
                     /locus_tag="PKJ16_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HNV46430.1"
                     /translation="MATVNKKDSSGAAELKKLRARVRALERTEKRLAQDLDALRESEN
                     IYRAIFNNTGTITLIFDENTIITMVNSDFAKRMGYSKREVEGKLSWTEFVAPEDLARL
                     KHYHELRSRDPKLAPRTHEFRIINRAGKMLNIFMTIEIIPGTRLRVASLIDITDRIRA
                     EEAIMQISGAERQKIGQDLHDDLAPHLIGIEVLVKILADSLERTSPADAPLIAKIRTL
                     LNEAISKTRRFAHGLCPVFLAEHGLESALKELSAATTDIYGVPCRFSFGEGVPKYDID
                     VSTHLYYIAQEAVHNAVKHGRATLIEIAVSAGGADTRLTVRDNGSGYDMSRHPDGIGH
                     KIMQYRSRVIGAYLDIWSAIGTGTRVTVTLVSENGDKGSAIT"
     gene            3603..4739
                     /locus_tag="PKJ16_05260"
     CDS             3603..4739
                     /locus_tag="PKJ16_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HNV46431.1"
                     /translation="MKVNDFMSTTGRNEPCPCGSGKKYKHCCLQEQDNVIPFPGVGFS
                     DDNFKRYEAFAESWDDAKGPVPTFMEFLGRPNAATESMKKLKQDIGDRIFESKEELQA
                     EIDRRMLSANTSPREEFLGLSPTQMRDVLHKSFLENTELVALDKSVDRELLEGVPVLA
                     QCRYVMQTIYETEPGYKPTQLGNFPRVLVQAFFERFIKEDALLKDPPMREDDVLELTR
                     LRFFLKDMGLMRKQQGRFRLTKKAEQILARNDPFELYSMLFDYLASSINWLYGTRYPE
                     DMVFIQHALVFNLLVLKRAASEFVPEQQLVDVFKRAFPTFESDMRAMSDFDMISSGFC
                     SLFLRRFAAVCGLVELRGDALVKLKRETEYRTTRLFDAVLVWKV"
     gene            complement(4762..5469)
                     /gene="rsmI"
                     /locus_tag="PKJ16_05265"
     CDS             complement(4762..5469)
                     /gene="rsmI"
                     /locus_tag="PKJ16_05265"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HNV46432.1"
                     /translation="MPGTLYIIGGPIGNLEDITLRALRILRECTDVIYCEDTRQTHKL
                     AAHYGIGAPLVSLHAHSSEAKLKKAVDDIAAGRNAVYLTDSGTPGLSDPGARFVAAAR
                     AAGVAVVPVPGPSALTALVSVAGLGGRRVLFAGFLSTKDGRRRRELERFRDIDGAIVV
                     YESPYRIKKLLRDIATVLPGARVAIGREMTKTYEEIITGSIEEIITGIDSLTDRGEFA
                     VAIAPGDVVISDAADGD"
     gene            5650..6735
                     /gene="serC"
                     /locus_tag="PKJ16_05270"
     CDS             5650..6735
                     /gene="serC"
                     /locus_tag="PKJ16_05270"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HNV46433.1"
                     /translation="MARIYNFSSGPSMLPLPALEEAAQKLVDFEGSGMSLVEMSHRGK
                     VYERVHNETIALFREILTVPNEFQVLFLQGGATLQFAMIPMAFLRAGDAADFILTGTW
                     AKKAHEDASLVGRANIIWDGKTSKYTRMPQQKELSFTSGARYVHLCSNETIGGVQWAS
                     YPDTAGVPIVADMSSDILSRQFPWNNIGLVFAGTQKNLAPAGMAIVVIRKDFIAAARK
                     DLPAYLRYDLHAENNSLYNTPPTFTVWATNLTLKWIKSMGGLEALERLNREKATLLYD
                     LIDASGGYYKNPVEKASRSLMNVVWLLDDDGLEDDFFKEAERQGLSGLKGHRSVGGCR
                     ASIYNAMPMEGVRALADFMKEFMKKHG"
     gene            6746..7111
                     /locus_tag="PKJ16_05275"
     CDS             6746..7111
                     /locus_tag="PKJ16_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HNV46434.1"
                     /translation="MEDNHRPWGFYDVLSDERDHKVKRITVYPDKRLSLQRHARRAEH
                     WYIVTGEALMTLGEREFTLCAGESVDIPCRAVHRIKNTGVANLVFIETQVGDYFGEDD
                     IERLEDDYSRAAPGGSSQQ"
     gene            7265..8212
                     /locus_tag="PKJ16_05280"
     CDS             7265..8212
                     /locus_tag="PKJ16_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV46435.1"
                     /translation="MITVNNLTKRFGDLVAVNDISFAIRKGEITGLLGPNGAGKTTTL
                     RMLTCYLAPDAGSVVVGDYLASMNPIEVRRIIGYLPESAPLYADMIVYDYLWYVGQIR
                     EIPAETLPTRIREIANVCGLAEVMHKNIGDLSKGYKQRVGLAHAMIHDPEILILDEPT
                     SGLDPNQIVEIRNVIKEIGKRKTVILSTHILSEVEATCDRVIIIDRGKIAADSTTREL
                     TEGGDTGNSVTLRVSGVEFGTLADAIRRLDGVSDIREAVDREGLTAANVLTGTASDVR
                     PSIFRLIADKGWTLYEMKQEQQTLENIFRKLTRGGEHEA"
     gene            8202..8918
                     /locus_tag="PKJ16_05285"
     CDS             8202..8918
                     /locus_tag="PKJ16_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HNV46436.1"
                     /translation="MKLDGSLIIMKKELRSYFYSPVAYIVIAAFLVLSGYLFFDTFFL
                     FGRSELRGFFELLPITFSIFIPALTMRLFAEEKHSGSFEILMTLPVTTLEAVLGKFLG
                     ATAFVGIMLAPTLFYAISVALVGSPDIGPIVGGYLGALLLGATFAAIGIFASTLTKNQ
                     IIALIVGVLACFLLTLFGLDRFKMLMPSALVGVVEYLSVSGHFHSIERGVVDSRDIVY
                     FLSVIALALMGSVHVLDERR"
     gene            8923..11055
                     /locus_tag="PKJ16_05290"
     CDS             8923..11055
                     /locus_tag="PKJ16_05290"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HNV46437.1"
                     /translation="MDIKQMLSQVKSLDAAGLAKRITQSLGARRSTLAINIVIILMIN
                     LVAGVLYFRCDATRNGAYSLSPLSRELVANLAEPLTVKVFFSKDLPAPYNGVYRYVSD
                     ILGEFKNSANRNFRYEFVDVEKQKDAAGDYGIYPVQVREIQSDQVKFRNAFMGIAIVH
                     GDLVEKIESITEAEGIEYRITTLIKKMIGKVDALVKLDGQIAVTFYASSSIPLRGIET
                     VNEQVEKIVRTVGAKNYNKLSYKHVDPGADKNQMALAESYGLFPLRWPAFTTDLGKRV
                     EAGSGVIGVVVEHGGRFETIQVLTRNIMGQIQVNTDTLADGLNAAIGNLISVNQKVGY
                     VTGHNERDINDERAGAANFKTLISDLYEFKTVDLSKDEIPDDINTLIVNGPKSRFSEY
                     ELFKIDQFLMKGRSVLFFLDSFDEMNMQGGGGMFGRQPLVIPVDTGLADYLKSFGVSV
                     NRDIVLDMSCYRTANQMFGEQVIYFAPMVNEESLDRANPITASLKRIFFLKGSSIALQ
                     EDALKSTNLTATTLVSSSRRSWLMEGRINFNPMMMQPPAETEMRSHKLAVLVSGQFES
                     YFKDRAEPPVKEEKGKAPAPGGSPIASSGIIKKAVKPGKILVAGTSEITGPSVVDKEG
                     KSPNAILLHNMVDYLAGNAAIPQMRSKGLEFNPLREAGDLKRYLLKGFNIAGVPLLVV
                     AAGLLIWRMRNMRRRRIQQEFTGGGSGE"
     gene            11048..12046
                     /locus_tag="PKJ16_05295"
     CDS             11048..12046
                     /locus_tag="PKJ16_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HNV46438.1"
                     /translation="MNKRVVISIALIVALGLVLAIKKARFGSAVPDIGKWSGEADEIV
                     IEGAGSSLRLHKKDGKWVINEAAYPADAKAVVKLENQLRDLELADLISEKPHLDRFDL
                     DAGKARRVVARRDKKPLRDILLGKKSSTYRHTYVKLADRPEVFLARGPLADDFAKSAD
                     DLRDKEVIKIDQASIESFEIEYKGARLSFAKKTVEIKDEKPAADPAAPKGTDAGKGAG
                     TNKDAKPVTVAKWTCAQLGGAELDEGKLNGILSSFASVVADAYAERPDPALMRARTCS
                     IKLRAGGKDMTLVIAGRETADRKRYLATSSESPYAFFLNAHGAERYFVTAADMKKK"
     gene            12073..13149
                     /gene="pyk"
                     /locus_tag="PKJ16_05300"
     CDS             12073..13149
                     /gene="pyk"
                     /locus_tag="PKJ16_05300"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HNV46439.1"
                     /translation="MAALTEILATIGPASVGIIDALYASGLSGVRINSSHGKPADHEA
                     VVRNSRRANPSGYIVYDIKGPKIRIGDIPEPLKITAGETLVIDTAFPKPPGSEYPAVK
                     DFSAGIPVTYERLHECVKPGHRLFIDDGYIGLRVESVEGTRIVCTVMYGDLLRQRKGL
                     NHPDTIVGYPYTMPYDIPNLEEAARMQVDFIADSFTRNADDVLELRDRLNGTGMRIIS
                     KIENPEGVENFDAILEKTDAIMVARGDLGVELDPWLIPELQKQMIEKCNAAGKPVITA
                     TQMLESMIDNPHPSRADVSDIANAIYDGTDVVMLSGETSVGKHPAECVAMMHRIAETV
                     EATDRYREKKIRRHTLAGLSGSKS"
     gene            13270..14274
                     /locus_tag="PKJ16_05305"
     CDS             13270..14274
                     /locus_tag="PKJ16_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46440.1"
                     /translation="MKLRMTLGVAAILVLISPANLQAQKCTSGNCANGTGTKDYGSYK
                     YTGEFRNGKKHGRGTVTLFSRTIKGAWRDDKLIHGEESYSDGFTLSGSYNEGFRSGKG
                     VMTWRDGRRYEGEWKDLTAVGKGVMTYPDKRRYVGEFQGDQYHGRGILYHANGSVYRD
                     GPWDKGVFKGVIKEKQQASTPTVYRPEKYGEISIRLSELPAWKFPYADAQGKYHYLIA
                     QEKCTYGLELEQWYQDRGFVESSHNKSNFCGKKMPPGYQVLVKPVLFVWGKQWSAAQI
                     AERKRIAESSKNRVTTNREKEYRGTYCWESCSKSCYSGSSTAKGQGMCLDSCRKQCSR
                     "
     gene            14611..15075
                     /locus_tag="PKJ16_05310"
     CDS             14611..15075
                     /locus_tag="PKJ16_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015850165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HNV46441.1"
                     /translation="MREIKLKAGDPAPDFTLKNQYDQDITLSGLKGKKVLLSFHPLAW
                     TPVCEIQMRTLEAKHDELERLNTVALGLSVDSPPCKKAWADYLDIDHTDLLADFWPHG
                     EVARRYDLFIEKAGISGRANILIDEQGKIARVWVYEIPEVPDIEDVLRFLKK"
     gene            15194..15277
                     /locus_tag="PKJ16_05315"
     tRNA            15194..15277
                     /locus_tag="PKJ16_05315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15228..15230,aa:Leu,seq:gag)
     gene            15358..16523
                     /locus_tag="PKJ16_05320"
                     /pseudo
     CDS             15358..16523
                     /locus_tag="PKJ16_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473722.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
     gene            16513..17937
                     /locus_tag="PKJ16_05325"
     CDS             16513..17937
                     /locus_tag="PKJ16_05325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HNV46442.1"
                     /translation="MHLDIVVIAAYLVAINVIGLYFAKSGSMNDYFLGARNIPWPVAC
                     LSIVATETSTLTFISIPGLAYATNMGFLQIAFGYLIGRVLVAVFMLPQYFQGNFETVY
                     QFIQNRFGVSSRRIIAIVFHVTRLLADSVRLFATAIPLTLFLGWPYWVSILVIGASTV
                     VYTLYGGIRSVAVTDAIQFFLYLSCACVGLIIICDIMGTDPLSIVRMVPGASLTVFST
                     GLADGARSLFGSYNIISGLVGGAFLSFASHGTDHLIVQRALICRDLPSAKKAMIGSGV
                     LIILQFALFLVFGLFIKALLEGRPFERSDEVLPFFIIPYLPEGLRGLMLAGIFAAAMS
                     TLSASINSLASSTSLDILALDRRDLPEKKKMRYAKGIALLWSVVVIGISLAFRDTKSP
                     LVEIGLSIASITYGGMMGMFLIGRVYDNFSERAAIGGMLASIAGTMVVVFATKVFWVW
                     YVVIGFAIAVAAGVAIQRIAGSTK"
     gene            18089..>20336
                     /locus_tag="PKJ16_05330"
     CDS             18089..>20336
                     /locus_tag="PKJ16_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46443.1"
                     /translation="MSFKRQIDKLRDRPRKLLIIFLALYNLHPFTYFLMVALVIGFAS
                     FLSIAKIIMILAPPIILSATVVFIVQNRRLQRYVNTLIDERDIPDLDAIQSFINSFPL
                     RAAVPLTVGCALGPVLTGVIGFAQGVFISAYQMVFVILLGEVTAIIVGAIMYFYTRVF
                     LYPSNRRIVFHPLTIFQKFSIPILSTVVVIVTMMILGIFKSIQSDTMEVQNRMLLESL
                     RHASSVTKAYIGIVTSEIGAFAKSDAARRGNLAEMQAILLALHRERGSSVEMYFFAAP
                     DGTAPTSVGITRNISDRGYFRQVMATGKPAVSDPVTNKATGKEILVVAVPVKRGDAVT
                     AVFGATILLDSLQVFYQTYASQSSRSHMLLSDAGKIVFSTDKELLGKTIGTDIVDDGV
                     RFRNVAALRTAPEWSTIDIVFKGRAMIAHKEDIPSIGFTLVSLADRTAVYAVLNPVLV
                     RSIVYLVLVIGIIFAIIWYLAKKISAPIENIIGIFQKVSDGDLTVRSTDYLADEFGEL
                     IRYLQILLRRLRETIHATVESSQQLAEASTGMSKTSLALSHNAQDQASSIEEMTAALE
                     ETSASIENFAKSAADQLSFATETYQAMERLKGQVIEIAQYAVEALEKATVSSEQARTG
                     NEHMKNAIDGMNRIDASTQKITEFVGLISDISDQVNLLALNAAIEAARAGEHGRGFAV
                     VADEIAKLADQTSQSAKSITGLVNEGRSEVIRGKDYVDATSRALSAIIENIRATDVLV
                     RRIAELSRS"
BASE COUNT         3973 a         6037 c         6311 g         4015 t
ORIGIN      
        1 gattgcgcac gcctcgagca ccttcggatg ggtgaagagc acctcgtcga tctcgcgcgg
       61 atagacattg tagccgccgg agatgaccat gtccttcagc cggtcgacga tgaagaaata
      121 gccgtcggcg tccatctcgg cgatgtcgcc cgtgtggagc cagccgtcgc ggatggtctc
      181 tttcgtctcg tcgggcatcc ccttgtagcg ctgcatcacc tgcggtccgc ggatgatgag
      241 ctcgccgggc ttcccgggcg ccacgtcggt cgcgcggtcc tcgatgtcga cgatgcggca
      301 ctcgacgtcc ggcaccggca cgccgatgct gccgggcttc ggccgtccct tctcgaaggg
      361 gttgatgtgc gtgaccggcg acgattcggt catgccgaag ccctcgcaga tgaccgtccc
      421 cgtcttttcc tcgaaccgtt tgatgacctc gaccgggagc ggcgcgcttc cgctgaagca
      481 gccgttcacg aacgagaggt cggtgcgctc gaactccgga tggttgatga tgccgatgta
      541 catggtcgga accagcggaa cgaatgtcgg cttgtccttc gtgagtactt ccatcagcgg
      601 atcgggctgc ggcttgggca ccaggatatt gttccaggcc ttgaacaccg cgaggttcat
      661 cgatacggtg aggccgaaca cgtggaagta gggaagggcg cctagcatgg tctcggcgcc
      721 gttgcgcatc tccgggaacc acgaagccgc ctgctgcacg tttgtgctga gattggcgtg
      781 cgtgagcacc acgcccttgg aaacgccggt ggtcccgccc gtgtactggt actgcgcgat
      841 ggcgtcgaac gacgcggccg gcgaatcagt cattggctgg gcgttgttga tgaaggtttt
      901 ccagcggaac acgttgggcg attgcttcac gtccgcggcg agtttcttct ttttcgcgac
      961 cagcggaaag aggatgctct tcgggaacgg gagatagtcg ccgatcgacg cgacgatgat
     1021 ctgcttgatg tgggtcttgg gccgcaggtc gatcatgcgg ttggccagaa gatcaagggt
     1081 caccagggcc ttcgcctcgg agtccttgag ctggtgctcg agctccgtgt ccgagtagag
     1141 cggattgttc atcacggcga tggcgccgat cttgagaatg ccgaagtatg cgaccacgca
     1201 ctggatggtg ttcggcagga tgatcgcgac gctgtcgccc ttcctgacgc catggccgat
     1261 gagcgcgccg gcaaatcggt ccaccatgcc cttgagctcg ctgtagttca gggtgaatcc
     1321 catgaaggtg agcgctttgt tcttcggaaa gccggccgtc gcacgctcga gaaactccgg
     1381 gagggtcttc ttctcgtagt tgatgttcgt tggcacgccg ggcgcgtatg atgtgagcca
     1441 gggcttgttg agatatgacg agttcttgct gacggtacgc ataaatgtct cccccatgat
     1501 attggtagtt tcctatttca tacggcggag cgtgcaaata gtaaagaggt gatggaggta
     1561 ttgagcggtg cgccgatgac ctattgccct gtaggtgaat cacgcacgac gatcaccgga
     1621 ggtgtcgctc acccagagcg cggcacgctt ggcgaggtcc gccgcggtct tgatgtcgag
     1681 cttctccttg atgcgctcgc ggtaggtccc gacggtcttg acgttgagcg cgagcttcct
     1741 cgcaatggcc cccgtaggga gccccttgcc gatgagcgtg aagatctcga gctcccggtc
     1801 ggtgagggtt tcgatgggac ccgacgcgga agccgccgtc cggtcggcga gccgctgcac
     1861 cagcgagtcc atgatcgccg cgctcacgta ggtgccgccg tccaggatct gacggatggc
     1921 gcggacgatt gaatcggagg tctcatgctt catgatgtag ccccgcgcgc cagcggcaag
     1981 cgcgcgctcg gcatagatgg cctcgtcgaa gagggagagc accagcatgg ggaggccctt
     2041 catgcatctg ttcacatcct tgatcagcat gatgccgttg ctgtccttca gcgatatgtc
     2101 gatgaccgcc atcgcgggcc gcgcttcgcg gatcagccgc agcgcgtcgg aggcattgtc
     2161 ggcctcgccg cagacgacaa ggtcctcctc ccggtcgatg agctccttca ggcccatccg
     2221 gaagatctcg tggtcctcga cgatgaagat cccgcgtcgc atggtcatgt gattgcgctc
     2281 cccttgtcgc cgttctccga caccagggtg acggtgacgc gcgttcccgt cccgatggcg
     2341 ctccagatgt cgagataggc gccgatcacc cgggagcggt actgcatgat cttgtggccg
     2401 ataccgtccg gatgccggct catgtcgtag cccgatccgt tgtcccggac ggtgaggcgc
     2461 gtatcggcgc cgccggcgga aaccgcgatc tcgatgagcg ttgccctgcc gtgcttgacc
     2521 gcgttgtgga cggcctcctg cgcgatgtag tacaggtgcg tcgatacgtc gatatcgtat
     2581 ttcggtacgc cctcaccgaa ggagaagcgg cacgggactc cgtagatgtc cgtcgtggcg
     2641 gcggagagct ccttgagcgc ggattcgagg ccgtgctcag ccaggaagac cgggcagagg
     2701 ccgtgggcga accggcgcgt cttcgatatg gcctcgttca gcagtgtgcg gatcttcgcg
     2761 atgagcggcg cgtcggccgg ggaggtgcgc tccaggctgt ccgcgagtat cttgaccaac
     2821 acctcgatgc cgatcaggtg cggcgcgagg tcgtcgtgca ggtcctgccc gatcttctgg
     2881 cgctcggcgc cgctgatctg catgatcgcc tcctcggccc ggatgcggtc cgtgatgtcg
     2941 atgagcgagg cgacccgcag ccgcgtcccc gggatgatct cgatcgtcat gaagatgttc
     3001 agcatcttcc cggcgcggtt gatgatccgg aactcgtgcg tgcgcggcgc cagttttgga
     3061 tcgcggcttc gcagttcgtg atagtgtttg agccgcgcga ggtcctccgg cgcgacgaac
     3121 tcggtccagc tcagtttgcc ctcgacctcc cgcttggagt agcccatccg cttggcgaaa
     3181 tcggagttca ccatggtaat gatggtgttc tcgtcgaaga tcagcgtgat ggtgccggtg
     3241 ttgttgaaga tcgcccggta gatgttctcc gattcccgga gcgcatcgag atcctgggcg
     3301 aggcgcttct cggtgcgctc gagcgctcgg acgcgtgcgc ggagtttttt cagttcggct
     3361 gcgccggatg aatccttctt attgaccgtt gccatggatg cctttatttt tcatgatatc
     3421 ccgttgttga aagggatgtc aagacattcc gggagaatgg gtgcgtgagc accacgagcg
     3481 gagagctgtt gtcccgactt tttgattgac gaattgtagt tttatcgtgt gatcattgtg
     3541 gaccttgaac ggatagtgag cgtcatgtca atgatcacta ttgcaggctc gttcaataat
     3601 aaatgaaggt gaatgatttt atgtcgacga ccggaagaaa tgaaccctgt ccctgtggaa
     3661 gcggaaaaaa atacaagcac tgctgcttac aggagcagga caacgtgatc cccttcccgg
     3721 gtgtcggctt ttcggacgac aattttaagc gctacgaggc attcgcggag agctgggatg
     3781 atgcgaaagg tccggtgccc acgttcatgg agttcctcgg gcgccccaat gcggcaacgg
     3841 aatccatgaa gaaactgaag caggacatcg gcgatcggat cttcgaatcc aaggaggagc
     3901 ttcaggccga aattgatcgt cggatgcttt ccgccaacac gagtcctcgt gaggagttcc
     3961 ttggattgtc gcccacgcag atgcgtgacg ttttgcacaa gtcgttcctg gaaaacacgg
     4021 aactagttgc gctcgacaag agcgttgaca gggaattgct ggagggcgta ccggtcctgg
     4081 cgcagtgccg atatgttatg caaacgatat acgagacgga accgggctac aagccgaccc
     4141 agcttggcaa tttcccccgc gttctcgtgc aggccttctt tgagcggttc attaaagagg
     4201 atgccctatt gaaagacccg ccaatgcggg aggatgatgt tctcgagctt acccggctgc
     4261 ggtttttcct caaggatatg ggccttatga gaaaacaaca ggggagattc agacttacca
     4321 agaaggcgga gcaaatcctc gcacggaatg atccgtttga gttgtactca atgttgttcg
     4381 attacctcgc ttcgtcaatc aactggcttt acggaactcg ctatcccgag gatatggtgt
     4441 tcatccagca tgcgctggtg tttaacctgc tcgtgctcaa gcgcgcggca tccgaattcg
     4501 tgcccgagca acaacttgtc gatgtgttta aacgggcatt cccaacattc gaatcggaca
     4561 tgagggcgat gagcgatttc gacatgattt catcgggatt ttgttcgctg tttctgagac
     4621 gattcgcggc ggtttgtggt cttgtcgaat tacggggcga tgcgttggtg aaactgaaac
     4681 gtgagaccga gtatcggacg acgaggttat ttgacgctgt tctcgtctgg aaggtgtaag
     4741 cggtaccggc cttccccttt attagtcgcc atccgcagcg tctgatatga cgacatcccc
     4801 cggcgctatc gccacggcga actctccgcg atccgtgaga ctgtcgatgc cggtgatgat
     4861 ctcctcgatg ctgccggtta tgatctcctc gtaggtcttg gtcatctccc ggccgatggc
     4921 gacgcgcgct cccggcagga ccgtcgcgat gtcgcgcagc agttttttga tgcgatatgg
     4981 cgattcgtac acgacgatgg cgccgtcgat gtcccgaaaa cgctcgagct cgcgccgccg
     5041 ccggccgtcc ttggtgctca ggaacccggc gaagagaacg cgccgcccgc cgagccccgc
     5101 gaccgagacg agcgccgtga gcgcggaggg gccggggacc ggtaccacgg cgacgccggc
     5161 cgcgcgcgcc gcggcgacga agcgcgcgcc gggatcggac agacctggcg ttcccgagtc
     5221 ggtcagatag acggcgttcc tgccggcggc gatatcatcc accgcctttt tgagcttggc
     5281 ctcggacgag tgcgcgtgga gcgacaccag cggcgcgccg atgccgtagt gggcggcgag
     5341 cttatgcgtc tgccgcgtgt cctcgcagta gatgacgtcg gtgcactccc ggaggatgcg
     5401 cagggcgcgc agggtgatgt cctccaggtt gccgatggga ccgccgatga tgtagagcgt
     5461 gccgggcatg attgttccgt gcgcataaat tttttcaccc gctatgaaac aaaaataaaa
     5521 tttcctgtca aatagtttaa tgtcttgacg ccggacgctt tcgcgtccat cacgggtgcg
     5581 tcgaattcct gaatgtggcc cgcacctcac gggtacacga taccaccaga cgcacacggg
     5641 cattgtgtta tggcgcgcat ctataatttt tcatccggtc cgtccatgct gccgctcccg
     5701 gcgcttgagg aggcggcgca gaagctggtc gatttcgagg gcagcggcat gtcgcttgtc
     5761 gagatgagcc accgcggcaa ggtctacgag cgcgtgcaca acgaaaccat cgcgctcttc
     5821 cgggagatcc ttaccgtacc gaacgagttc caggtgctct ttctgcaggg cggcgcgacg
     5881 ctccagttcg cgatgatccc gatggcgttc ctgcgagcgg gcgatgccgc cgatttcatt
     5941 ctcaccggca cgtgggccaa gaaggcgcac gaggacgcct cgctggtggg acgagcgaac
     6001 attatctggg acggcaagac gtcgaaatac acccggatgc ctcagcaaaa ggagctcagc
     6061 ttcacgagcg gcgcgcgcta cgtgcaccta tgctcaaacg agaccatcgg cggggtgcag
     6121 tgggcatcct acccggacac ggcgggcgtt cccatcgtgg ccgacatgtc gtcggatatt
     6181 ctgtcacggc aatttccctg gaacaatatc ggcctggtgt tcgccggcac gcaaaagaac
     6241 cttgcgccgg cggggatggc gatcgtcgtc atccgaaagg actttatcgc ggcggcgcga
     6301 aaggaccttc cggcgtacct gcgctacgat ctgcacgccg agaacaactc cctctacaat
     6361 acgccgccca ccttcacggt gtgggcgacc aatctcacgc tcaaatggat caagtccatg
     6421 ggcggcctgg aggcgctgga gcggctcaat cgggagaagg cgaccctcct gtatgatctc
     6481 atcgatgcaa gcggcgggta ttacaaaaat cccgtggaaa aggcaagccg atccctcatg
     6541 aacgtggtgt ggctcctgga cgacgacggg ctcgaggacg actttttcaa ggaagccgaa
     6601 cgacagggct tgagcggcct caagggtcac cgctcggtgg gcggatgccg cgcgtcaata
     6661 tacaatgcca tgcccatgga gggcgtgcgc gcgcttgccg atttcatgaa ggaattcatg
     6721 aaaaagcacg gctagggagg gcggcatgga agacaatcat cgcccctggg gcttctacga
     6781 cgtgctctcc gacgagcgcg accacaaggt gaagcgcatc acggtgtacc ccgacaagcg
     6841 cctgagcctg caacgacacg cgcggcgcgc ggagcactgg tacatcgtga ccggcgaggc
     6901 gctgatgacg ctcggcgagc gcgaattcac gctgtgcgcc ggagagtcgg tggacatccc
     6961 ctgccgcgcc gtgcaccgca tcaagaacac cggcgtcgca aacctcgtct tcatcgagac
     7021 gcaggtgggc gactacttcg gcgaggacga catcgaacgc ctggaagacg attacagccg
     7081 ggcagcgccg gggggatcgt cgcaacagta aagacgtggc agtgccgtat cagtaaagac
     7141 ggggcattgc cccgtctcga ctctgggatc gatacaatca taaagacgag gcactgcctc
     7201 gtctcacagg aagagtacgg gtggggcgat gccccactcc aaccatggag gacaacacga
     7261 ccgcatgatt accgtgaaca acctcaccaa gcgtttcggc gatctcgtcg cggtgaacga
     7321 catctcgttc gcgatccgca agggcgagat cacggggctg ttgggcccca acggcgcggg
     7381 gaagacgacg accctgcgca tgctcacctg ctatcttgcg cccgacgcgg gaagcgtggt
     7441 cgtgggcgat tacctcgcga gcatgaatcc catcgaggtg cgacgcatca tcggctacct
     7501 gccggaatcg gcgccgttgt acgccgacat gatcgtgtac gactacctgt ggtacgtagg
     7561 tcagatccgc gagatccccg cggagacgct cccgacgcgc atcagggaga tcgcaaacgt
     7621 ctgcggcctg gccgaggtga tgcacaagaa catcggcgac ctctcgaagg gctacaaaca
     7681 gcgcgtgggg ctcgcgcacg cgatgataca cgatcccgag atccttatcc tggacgagcc
     7741 gacgagcggg ctcgatccga accagatcgt cgagatccgt aatgtgataa aagagatcgg
     7801 gaagcgcaaa acggtgattc tctccacgca tatcctttcg gaggtggagg ccacctgcga
     7861 ccgcgtcatc atcatcgatc gcgggaagat cgcggccgac agcaccacgc gcgagctcac
     7921 cgaggggggc gacacgggaa acagcgtgac gctgcgcgtg agcggggtcg agttcggcac
     7981 gctggccgac gccatccgcc gcctggacgg cgtgagcgac atccgcgagg cggtggaccg
     8041 cgagggcctt acggccgcca acgtgctcac cgggacggcg agcgacgtgc gcccgtcgat
     8101 cttccggctc atcgccgaca agggctggac gctctatgaa atgaagcagg agcagcagac
     8161 ccttgagaac atctttagaa aactcaccag gggaggggag catgaagctt gatggttctc
     8221 tcatcatcat gaaaaaggag ctgcggagtt atttctactc tccagtcgcc tatatcgtca
     8281 tcgccgcgtt tctcgtcttg agcggctacc tgttcttcga tacgttcttt ctcttcggcc
     8341 gctccgaact gcgcggcttc ttcgagctgt tgcccatcac cttttcgata ttcatcccgg
     8401 cgctcacgat gcgcctcttt gccgaggaga agcacagcgg ctcattcgag atcctcatga
     8461 cgctgccggt gaccacgctg gaggcggtgc tgggcaagtt cctgggggcc acggccttcg
     8521 tagggatcat gctggcgccc acgctcttct acgcgatctc ggtggcgctg gttggatcgc
     8581 ccgacatcgg gcccatcgtg ggcggctacc tgggtgcgct cctcctgggc gcgaccttcg
     8641 cggccatcgg gatcttcgcg tccaccttga cgaagaacca gatcatcgcg ctcatcgtgg
     8701 gcgtcctggc ctgctttctg ctcacgctct tcgggctgga ccgcttcaag atgctcatgc
     8761 ccagcgccct ggtgggcgtc gttgagtacc tgtcggtgtc ggggcacttt cacagcatcg
     8821 agcgcggggt ggtcgattcg cgcgacatcg tgtatttcct ttcggtgatt gcgctggccc
     8881 tcatgggctc ggtgcacgtc ctggacgagc ggaggtaggg ccatggacat caagcaaatg
     8941 ctgtcgcagg tgaaatcgct ggacgcggcg gggctcgcga aacggatcac gcagtcgctg
     9001 ggcgcgcgcc gctccacgct cgccatcaac atcgtcatta tcctgatgat caacctcgtc
     9061 gcgggggtgc tctacttccg ctgcgacgcg acgcgcaacg gcgcctactc gctctcgccg
     9121 ctgagccgcg agctcgtcgc gaacctcgcc gagccgctca ccgtgaaggt gttcttctca
     9181 aaggacctgc ccgcgcccta caacggcgtg taccgctacg tctccgacat cctcggggag
     9241 ttcaaaaaca gcgcgaaccg caacttccgc tatgagttcg tggacgtgga gaaacagaag
     9301 gacgccgcgg gagactacgg catctatccc gtgcaggtgc gcgagatcca gagcgaccag
     9361 gtgaagttcc gcaacgcctt catgggcatc gcgatcgtcc acggtgacct ggtggagaag
     9421 atcgagtcga tcaccgaggc cgagggcatc gagtaccgca tcaccacgct tatcaagaag
     9481 atgatcggca aggtggacgc gctcgtgaag ctcgacgggc agatcgccgt gaccttctac
     9541 gccagctcct cgatccccct gcggggcatc gagaccgtga acgagcaggt ggagaagatc
     9601 gtgcgcacgg tgggcgccaa gaattacaac aagctgtcct acaagcacgt ggaccccggc
     9661 gcggacaaga accagatggc gctggccgaa tcgtacggcc tctttccctt gaggtggccc
     9721 gccttcacca cggaccttgg caagcgcgta gaggccggct ccggcgtgat cggcgtggtg
     9781 gtggagcacg gggggcgctt cgagaccatc caggtgctca cgcgcaacat tatggggcag
     9841 attcaggtga acaccgacac gctcgcggac gggctcaacg cggccatcgg gaacctcatc
     9901 agcgtgaacc agaaggtggg gtatgtgacc ggccacaacg agcgcgacat caatgacgag
     9961 cgcgccggcg cggcgaactt caagacgctc atctccgacc tctacgagtt caagacggtc
    10021 gacctgtcga aggacgagat ccccgacgac atcaacacgc tcatcgtgaa cgggcccaag
    10081 tcccgcttca gcgaatacga gctctttaaa atcgaccagt ttctcatgaa gggacgatcg
    10141 gtgctgtttt tcctggattc cttcgacgag atgaacatgc agggcggcgg cgggatgttc
    10201 ggtcgccagc cgctggtgat ccccgtggat acgggcctcg ccgattacct gaagtccttt
    10261 ggcgtgagcg tgaaccgcga tatcgtcctc gacatgtcgt gctaccgcac cgccaaccag
    10321 atgttcggcg agcaggtgat ctacttcgcg cccatggtca acgaggagtc gcttgaccgc
    10381 gcaaacccca tcaccgcatc gctgaagcgc atcttctttc tgaagggctc gtccatcgcg
    10441 ctgcaggagg acgcgctcaa atcgacgaac ctcacggcca cgacgctggt gtcctcgtcg
    10501 cggcgaagct ggctcatgga ggggcgcatc aacttcaatc ccatgatgat gcagccgccg
    10561 gccgagaccg agatgcgcag ccacaagctc gccgttctcg tgtccggcca gttcgagagc
    10621 tatttcaagg accgggccga gccgccggtt aaagaggaga aggggaaagc gcccgcgccg
    10681 ggcggatcgc ccatcgcctc gtcgggcatc atcaagaagg ccgtgaagcc cggaaaaatc
    10741 ttagtggccg ggacctccga gatcaccggg cccagcgtgg tggacaagga gggcaaatcg
    10801 cccaatgcga tcttgctgca caacatggtc gactaccttg cgggcaacgc cgcgatcccg
    10861 cagatgcgca gcaaggggct cgagttcaat ccgctgcgcg aagcggggga cctgaagcgc
    10921 tatctgctca agggcttcaa catcgcgggc gtgccgctgt tggtggtggc ggccggcctc
    10981 ctcatctggc ggatgcgcaa catgcgacga cggaggatac agcaggaatt cacgggaggt
    11041 ggatcgggtg aataaacggg tcgtcatcag catcgcactc atcgtcgccc tggggctcgt
    11101 gctcgcgata aaaaaagcgc gcttcggatc ggcggtgccg gacatcggga aatggagcgg
    11161 cgaggccgac gagatcgtga tcgaaggcgc cgggagctcg ctgcgattgc acaagaagga
    11221 cgggaagtgg gtcatcaatg aagcggccta tccggccgac gcgaaggcgg tcgtgaagct
    11281 tgaaaaccag ctgcgcgatc tcgagctcgc cgacctcatc tcggagaagc cgcacctcga
    11341 ccgcttcgac ctcgatgcgg gcaaggcccg gcgcgtcgtg gcgcgtcgcg acaagaagcc
    11401 gctgcgcgac atcctgctcg gcaagaaaag ctcgacatat cggcacacgt atgtgaagct
    11461 cgcggatcgg cccgaggtct ttctggcgcg gggtccgctc gcggacgatt ttgcgaaatc
    11521 cgccgacgat ctgcgcgaca aggaggtcat aaagatcgac caggcctcga tcgagtcctt
    11581 cgagatcgag tacaagggcg cgcggctttc attcgcgaaa aagacggtgg aaatcaagga
    11641 cgaaaagccg gcggctgatc ctgccgcgcc gaagggaacg gacgcgggga agggcgccgg
    11701 cacgaacaag gacgcgaagc ccgtgaccgt cgccaaatgg acctgcgcgc agctgggcgg
    11761 ggccgagctc gacgagggaa agctcaacgg catcctgagc tcgttcgcgt cggtggtggc
    11821 cgatgcatac gccgagcgtc ccgatcccgc gctcatgcgc gcccggacct gttcgatcaa
    11881 acttcgcgcg ggcggcaagg acatgacgct cgtgatcgcc ggacgggaaa ccgccgaccg
    11941 gaagcgctat ctggcgacat catcggaatc accctacgcc tttttcttga acgcgcatgg
    12001 cgcagaacgg tatttcgtga ccgccgccga catgaagaaa aaataggcgc gcagtctcac
    12061 gggaggtcgg acatggcggc attgacggag atactcgcga ccattggacc ggcctcggtc
    12121 ggcattatcg acgcgctgta tgcgagcggc ctctccggcg tgcgcatcaa cagctcgcat
    12181 gggaagccgg cggaccacga ggcggttgtg cgaaactcgc ggcgcgccaa tccctcgggc
    12241 tatatcgtgt acgacatcaa gggcccgaag atccgcatcg gcgacatccc cgagccgctc
    12301 aagatcacgg cgggcgaaac gctcgtcatc gacaccgcct tccccaagcc tcccggctcg
    12361 gagtatcccg cggtgaagga cttttccgcg gggatccccg tcacctatga gaggctccac
    12421 gagtgcgtga agcccgggca ccggctcttc atcgacgacg gctacatcgg cctgcgcgtg
    12481 gagtccgtcg aggggacgcg catcgtgtgc acggtgatgt acggcgatct cctgcgccag
    12541 cggaaggggc tcaatcatcc cgacacgatc gtgggctatc cctacaccat gccgtacgac
    12601 atccccaacc tggaggaggc ggcgcgcatg caggtcgatt tcatcgccga ttccttcacg
    12661 cgaaacgccg atgacgtgct cgagctgcgc gatcgtctca atggcaccgg catgcgcatc
    12721 atctcgaaga tcgagaaccc cgagggcgtg gagaacttcg acgccatcct cgagaaaacc
    12781 gacgctatca tggtggcacg cggcgacctc ggcgtggagc tggacccctg gctcatccct
    12841 gagctccaga aacagatgat cgagaagtgc aatgccgccg gcaagcccgt catcaccgcg
    12901 acccagatgc tggagtcgat gatcgacaat ccgcacccca gccgcgccga cgtgagcgac
    12961 atcgccaacg ccatctacga cggcaccgat gtggtgatgc tctccggcga gacctccgtt
    13021 ggcaaacacc cggccgagtg cgtcgcgatg atgcaccgga tcgcagaaac ggtggaggcc
    13081 accgaccgct atcgcgagaa gaagatccgg cggcacacgc tcgcgggttt gagcggatcg
    13141 aaatcttagc cggccatttt atcgttcgcg acatcggtga agcaagatgc cggtgcgttc
    13201 gttccgtagg attcggccat ggagtagcgg ctgattggtg agctttctgg tggggggtgc
    13261 gcgatgacaa tgaaactccg catgacgctt ggcgttgccg cgatacttgt tcttatctcg
    13321 cctgcgaatc ttcaggcgca aaaatgtaca tcgggaaact gcgcgaacgg tacggggacc
    13381 aaagactacg gttcgtacaa atataccgga gaatttcgca acggcaaaaa acatggtcgg
    13441 gggaccgtca cgctcttcag ccgcaccatc aaaggcgcct ggcgggatga caaactaatc
    13501 catggcgagg aatcatacag cgatggattt acgctctcag gttcttataa cgaggggttc
    13561 cgctccggga aaggcgtgat gacctggcgt gacggcaggc gatatgaggg cgaatggaag
    13621 gatttgacgg ctgtcggtaa aggcgtgatg acctatccgg acaagagaag atatgtcggc
    13681 gagtttcagg gcgatcaata tcatggcagg ggcatattat atcatgccaa cggctcggtg
    13741 taccgggacg gcccctggga caaaggcgta ttcaagggcg tcatcaaaga gaagcagcag
    13801 gcgagcactc cgacggtcta tcggcccgag aaatacggcg agatatcgat tcgactgtcc
    13861 gaactgcccg catggaaatt tccatatgcc gatgcccagg ggaaatacca ttacctgatc
    13921 gcccaggaga aatgcaccta cgggctcgag cttgagcagt ggtaccaaga ccggggattc
    13981 gtcgagtcct cccataataa aagcaatttc tgcgggaaaa agatgcctcc cggctaccag
    14041 gtgctcgtca agccggtcct gttcgtgtgg ggcaagcaat ggtcggcggc tcagattgcg
    14101 gagcggaagc ggatagcgga atcatccaag aaccgcgtta cgacgaaccg tgaaaaagaa
    14161 taccggggca cgtactgctg ggagagctgc agcaaaagct gctactcggg ttccagcacc
    14221 gccaagggcc agggcatgtg cctggacagc tgcaggaaac aatgttcccg gtagtggatt
    14281 ttgaacctgt ttcagtaggg agctgaatga gacgacagga tatcacgctc gctccgccgc
    14341 gaacaataca tccatgtatt cgcggctccg gcagccaatc cgggctgcct gctcgctgtg
    14401 acatccgaca gactcattcg gcactattgg gaaaaaatca acttaatagg ctttagggta
    14461 agggcttacc ttcgccacat catcgtccgt ttaataattc tggaaatatg ggctgtttgt
    14521 tactatagtg tttttagcag cgtcgtatga tccggcattc cggatccggt gcgacgccgc
    14581 aagtccatcg acatcaaagg gggtgcgcgc atgagagaga taaaattgaa agccggtgat
    14641 ccggcgccag attttacgct gaagaaccag tatgaccagg acattaccct gtccggcttg
    14701 aaggggaaga aggtcctgct gtcgttccat ccgctcgcct ggacgcccgt ctgcgaaatc
    14761 cagatgcgga cgctcgaggc gaagcacgac gagctcgagc ggctcaacac cgtcgccctg
    14821 gggctgagcg tcgatagtcc tccctgcaag aaggcctggg ccgattatct cgacatcgac
    14881 cacactgacc tcttggcgga cttctggccg cacggggagg tcgcacggcg gtatgatctg
    14941 ttcatcgaga aggccgggat ctccggcagg gccaacatcc tcattgacga gcagggaaag
    15001 atcgcgcgcg tgtgggtgta tgagattccc gaggtgcccg acatcgagga cgtcctcagg
    15061 tttctgaaaa aatgaagtga cgattcacgg cctcctcttc atcgcgggaa ggaattgacg
    15121 acggttttcc cggctggtgt gaatttttct tgttgacaat accggcgcgt gaaatatctt
    15181 ggataaacct caggcgcagg tggtggaact ggtagacacg tatgtttgag gggcatatgg
    15241 ggtaaaacct gtgcgggttc aagtcccgcc ctgcgcaacg gagaaaaggc gcggatgata
    15301 tccgcgcctt ttctattggc attgacatca ttccgaccgc acgatacaag acgcgacatg
    15361 gtcatcaccg gactggaaaa actgctgaac gatcccggcg cgtatcggaa gcggcgcgtc
    15421 gccctcatcg caaaccagac gtcggtgacg agcgatctcg tctatagttg gcacgcgctc
    15481 atccggcacg gcatcgacat cgtccgtatc ttctcgcccg agcacgggat cttcgccacc
    15541 gagcaggacc aggcgccggt gggcgaccag cccatcgtca attgcgaggt cgtgagcctg
    15601 tatggcgcat cggcgcacac cctcgccccg gcgccggagt ttctcgacga catcgattgc
    15661 gtcatctacg atattcagga catcggctca cgctactata cctacgtgaa cacgatggcg
    15721 ctgtgcatgg aggccatcgc ggggcgcgac atcgagtttg tggtgctgga ccggcccaat
    15781 cccatcggcg gacagtcggt cgagggtccg ccgctgcgtg atggctatgg gtccttcgtg
    15841 ggggtgctgc cggtccccgt gcgtcacgga atgaccgcag gcgagcttgc gcttctgtgg
    15901 aaggcgcacc ggcggctcga tgtgaacctc gcgatcgtcc cgatggacgg gtggggccgc
    15961 gcgatgggat atgcgcacac cggcctgccg tgggtgccgc cgtcgcccaa catgcccacc
    16021 gaggagaccg cgcttgtcta tcccgggatg tgcctcatcg agggcaccaa tctgtcggaa
    16081 gggcggggca cggcaatgcc attcctgcag atcggagcgc cgtacgtcga cgctgcggcg
    16141 cttgcaaccg gctcaacgcg ctcaatcagc ccggcatccg ctttcggccc gtatatttca
    16201 agccaacgtc gaacaagtac gcgggggagg tcgcgggcgg ggtgtttctg catgtgaccg
    16261 accccacgcg cttccggccg ttcgccgccg gggtggcgct cgtgcaggcg gcccgagagc
    16321 tctgtaccga ttcgttccga ttcacgacgg gcgtgtacga attcaacgac actcaccccg
    16381 cgttcgatct tcttgccggg agcgatgccg tgcgcaccat gatcgagcgc ggcgtgccgc
    16441 tcgatacgat acgagaatcg tgggcggatt acgaacggga ctttttcgaa cgaaggaagg
    16501 aatatttgct ctatgcatct tgatattgtc gtcatcgccg cgtaccttgt cgcgatcaac
    16561 gtcatcggac tgtatttcgc caagtcgggg tccatgaacg actactttct gggcgcgcgg
    16621 aacatcccct ggccggtggc ctgcctttcc atcgtggcca cggagaccag cacgctcacc
    16681 tttatctcca tccccggcct cgcctacgcg accaatatgg ggtttctgca gattgccttc
    16741 ggctacctta tcgggcgcgt gctggtcgcc gttttcatgc tcccgcagta ttttcagggc
    16801 aatttcgaga cggtgtacca gttcatccag aaccggttcg gcgtctcgtc gcggcggatc
    16861 atcgccatcg tgtttcacgt gacgcggctc ctggccgaca gcgtgcgcct gttcgcgacc
    16921 gccatcccgc tcacgctctt tctgggctgg ccgtactggg tgtccatcct ggtgatcggc
    16981 gcgtccaccg tggtgtacac gctctacggc ggcatccgct cggtcgcggt caccgacgcc
    17041 atccagtttt ttctgtatct ctcgtgcgcg tgtgtgggct tgatcattat ttgcgacatc
    17101 atggggacgg acccgctctc gattgtgcgc atggtccccg gggcgagcct cacggtcttc
    17161 tccacgggcc tcgcggatgg cgcgcgaagc ctcttcggct cctacaatat catctcggga
    17221 cttgtcggcg gcgccttcct gtcgttcgcc tcgcatggaa cggaccatct catcgtgcag
    17281 cgcgcgctca tctgccgcga cctgccgtcg gcgaagaagg ccatgatcgg cagcggggtg
    17341 ctcatcattc ttcagttcgc gctctttctc gtcttcggct tgttcatcaa ggcgctgctg
    17401 gagggccgtc ccttcgagcg cagcgacgag gtgctgccgt tcttcatcat cccctacctc
    17461 ccggagggcc tgcgggggct gatgctggcg ggcatcttcg ccgccgccat gtccacgctg
    17521 agcgcctcga tcaactcgct cgcatcgtcc acctccctgg acatcctggc gctcgaccgc
    17581 cgtgatcttc ccgagaaaaa aaagatgcgc tacgccaagg gcatcgcgct cctctggagc
    17641 gtcgtcgtca tcggcatctc gctcgcgttc cgcgatacga agagcccgct ggtggagatc
    17701 ggcctgtcca tcgcctcgat cacctacggc ggaatgatgg gcatgttcct catcggccgc
    17761 gtgtacgata atttctccga gcgagcggcg atcgggggca tgctcgcgag catcgccggc
    17821 accatggtcg tggtgttcgc cacgaaggtc ttctgggtgt ggtatgtggt gattggcttc
    17881 gctattgcag ttgcggccgg tgtcgccatc cagcggatcg ccggctcgac gaagtgatca
    17941 tccgcgcgcg gtttgtttaa cttttatgta ataatatgag tataataaaa aaaagtccca
    18001 ttttcgggga ctggaaagtt tttttcttgt ttatggcggg tagttactat atactctcgg
    18061 ttgatgataa tatcgtggag agtatgagat gtctttcaaa cgccagattg acaaactcag
    18121 ggaccggccg cgcaagctgc tgataatctt tctcgcactg tacaacctgc atccgtttac
    18181 ctatttcctg atggttgcgc tcgtcatcgg gttcgcgagt tttctgagta tcgcgaaaat
    18241 catcatgatt ctcgcgccgc ccatcatcct gtcggcgacc gtggtgttca tagtccagaa
    18301 ccgacgcctg cagcggtacg tcaatacgct catcgacgag cgcgatattc ccgatctgga
    18361 cgcgatccag tcgtttatca actccttccc gctgcgcgcc gcagttcccc tcacggttgg
    18421 gtgcgcgctc ggaccggtcc tcacgggcgt catcggcttc gcccagggcg tgttcatctc
    18481 cgcttatcag atggtgttcg tgattttgct cggggaggtc accgccatca tcgtgggcgc
    18541 gatcatgtat ttttacacgc gcgtgttcct ctatccgtcc aaccggcgca tcgtgtttca
    18601 cccgctcacc atatttcaaa aattctcgat tcccatcctc tcgacggtgg tcgtcattgt
    18661 cacgatgatg atcctgggga tattcaagag catccagtcc gacaccatgg aggtgcagaa
    18721 ccggatgctc ctcgagtcgc tgcgccacgc ctcaagcgtg accaaggcat acattgggat
    18781 agtgacctcc gagatcggcg cgttcgccaa atcggacgcc gcaagacgcg ggaacctcgc
    18841 cgagatgcag gcgattctcc tcgcattgca ccgcgaacgg gggagctccg tcgagatgta
    18901 ttttttcgcc gcgccggacg ggacggcgcc gaccagcgta ggtatcacga gaaacatcag
    18961 cgaccgcggc tacttccggc aggtcatggc gacgggcaag ccggccgttt ccgatccggt
    19021 caccaacaag gccaccggca aggagattct ggtcgtcgcc gtgccggtca agcgggggga
    19081 cgccgttacg gccgtcttcg gcgcgaccat tttgctcgat tccctgcagg tgttctatca
    19141 aacctatgcc tcccaatcgt cacggtcgca tatgctcctc agcgacgcgg gaaagatcgt
    19201 cttttcgacg gacaaggagc ttctgggtaa aacgatcgga acggacattg tcgatgatgg
    19261 ggtccgcttt cgcaatgttg cggcgctgcg cacggcgccc gaatggtcta cgatcgacat
    19321 cgtattcaag ggcagggcga tgatcgcgca caaggaggat attccctcga tcgggtttac
    19381 gctcgtgtcg ctggccgacc ggaccgcggt gtacgccgtg ctcaatccgg tccttgtccg
    19441 gtcgatcgtg tatctcgtgc tggtaatcgg catcatcttc gcgatcatct ggtatcttgc
    19501 gaaaaagata tccgcgccca tcgagaacat catcggcatc ttccagaagg tctccgacgg
    19561 cgacctcacg gtgcgctcca ccgactacct cgccgacgag ttcggcgagc tgatccgcta
    19621 cctgcagatc ctgctgcgcc gcctgcgcga gaccatccac gcgaccgtcg aatcgtcgca
    19681 gcagctcgcc gaggcctcca ccggcatgtc gaagacgagc ctggccctct cgcacaacgc
    19741 gcaggatcag gcctcgtcca tcgaggagat gaccgcggcg ctcgaggaga cctcggcgtc
    19801 catagaaaac tttgcgaaaa gcgcggccga ccagctctcg ttcgccaccg agacctatca
    19861 ggccatggag cgcctcaagg ggcaggtcat cgagatcgcc cagtacgccg tggaggcgct
    19921 cgaaaaggcg accgtctcca gcgagcaggc gcgcacgggc aacgagcaca tgaagaacgc
    19981 gatcgacggc atgaaccgga tcgacgcgag cacgcaaaag atcaccgagt tcgtggggct
    20041 catcagcgac atctccgacc aggtgaacct gctggcgctc aacgccgcca tcgaggccgc
    20101 gcgcgccggc gagcacgggc gcgggttcgc cgtggtggcc gacgagatcg ccaagctcgc
    20161 cgaccagacc tcgcagagcg cgaagagcat caccggcctg gtgaacgagg ggcgaagcga
    20221 ggtgatccgc ggcaaggact acgtggacgc gacctcgcgg gcgctctcgg cgatcatcga
    20281 gaacatccgc gccaccgacg tcctggtgcg gcgcatcgcc gagctgtcgc gctcgc
//
LOCUS       DAORHF010000039        20304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1818_length_20304_cov_5.192602, whole genome shotgun sequence.
ACCESSION   DAORHF010000039 DAORHF010000000
VERSION     DAORHF010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_20304_cov_5.192602"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..628
                     /locus_tag="PKJ16_05335"
     CDS             53..628
                     /locus_tag="PKJ16_05335"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HNV46444.1"
                     /translation="MLPTIITSSELFSMLFIFFARIIDVSLGTMRIILIARGYRSLAP
                     IFGFLEVFIWLGAIGTTISNLHSIFSYFIYAGGFAAGNYVGMLIEERVSIGYQLIRII
                     TTKEVTALPMVMREEGYGVTVVDGKGMRGDVYILYAVVRRSEVKKILGIVETFEPSAF
                     ITVEDVRSHIAGFIGDKGGPPFFGRMFAKRK"
     gene            complement(640..1788)
                     /gene="pgsW"
                     /locus_tag="PKJ16_05340"
     CDS             complement(640..1788)
                     /gene="pgsW"
                     /locus_tag="PKJ16_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HNV46445.1"
                     /translation="MKKILWKPTTVSWQIHVIIALVAIIGLAMVELFRVNVKQPHYEM
                     KYNAARTMKRAMDVIKEHRQQEDLPPIDRKIDPTASGLIGIQTSPITSAPVDIAAKVA
                     TLNPNWAAVVVQMLRESKVRTGSTVAVSFTGSFPALNCAVLAAGEAMKLRMIVITSVS
                     SSTWGANIPAFTWLDMERLLVQKNIIKSRSVAASLGGVQDNARGQSEEGRRLIREAIR
                     RNGLPIIEREDLKANIDSRMDLYYERAGDKTIDAFINVGGGTVVIGSYIGKMRYKPGV
                     NLRPSRKAMEIESVISRFARAGVPVIHLDSLLTIANQYGLPYTLKAMPKVGVGEIFYR
                     LEYNRLLVAGVLAVLVGLILVLIKMGYGYRMFSRRQQDTRQRPPEHMV"
     gene            complement(1785..2243)
                     /gene="pgsC"
                     /locus_tag="PKJ16_05345"
     CDS             complement(1785..2243)
                     /gene="pgsC"
                     /locus_tag="PKJ16_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HNV46446.1"
                     /translation="MENILIVSIGLGLAVSLLFSEMLGIAAGGMVVPGYIALSINKPI
                     MILTTIAVSFLTFFIVHSMSAFMIIYGRRRTVLMILVGFLLGWFTRSIGVIPIGKQPL
                     DLTIVGYIIPGLIAIWMDRQGVLETVTALVTSSVIVRLALILIFGREIGQ"
     gene            complement(2243..3454)
                     /gene="pgsB"
                     /locus_tag="PKJ16_05350"
     CDS             complement(2243..3454)
                     /gene="pgsB"
                     /locus_tag="PKJ16_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HNV46447.1"
                     /translation="MTGITILVLLIGVLVGLGIVEYSLHRRNLRAIPIRIHVNGTRGK
                     SSVTRLIAAGLRAGGITTIAKTTGTLPRVIVDDGTEYPVFRQSRPNIIEQLRIVAFAA
                     RNRARALVIECMALQPHLQSLSEQKMIRATHGVITNVREDHLDVMGPTERDVALAILG
                     TTPNDAVLFTAEQDYPDEFAAVCAQRNTRCVFVGEDAVRALEASDMARFSYLEHPENV
                     ALALSVCEAIGVERDVALAGMQSVAPDPGAMSDLRMNFFGRDICFINGFAANDPESFE
                     RIWRMALKSHSAAKGRIMVINTRIDRPDRSRQFGEALASWPAADKYVLIGSGSYFLVK
                     YAVRSGIDPRVFVNAEGLDAESIFEEILSHCRESTLVMGVGNIAGPGLELIKYFKNRS
                     TVMAYGGAAEG"
     gene            3779..4336
                     /locus_tag="PKJ16_05355"
     CDS             3779..4336
                     /locus_tag="PKJ16_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNV46448.1"
                     /translation="MDIGPILKKIRLERHYTLSQVAGKIGSTASLLSQIENCKISPSL
                     HSLESLLKCYSVSLSDFFRQVEQKNYIFLKRSESETFSNPDEGYRLTLLASKLQNNTL
                     ESYIVEIDPDRGISLATTGKELNGERLILIFSGQAQVEMSDEVILAMAEGDSANFKSY
                     VPCTIRNTGADVARMLISGMPPIIL"
     gene            complement(4343..5275)
                     /locus_tag="PKJ16_05360"
     CDS             complement(4343..5275)
                     /locus_tag="PKJ16_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46449.1"
                     /translation="MSGQPSVAVQHLTKNFGAFTAVNDISFEVRPGEIFGFLGPNGAG
                     KSTTIKMLCGIIAPSSGTGRVLDFDILADQDAIKTRIGYMSQKFSLYGDLTVYENLEF
                     FGSIYGLSGALLAERMAYALEMAEIAGRKDAIVGSLPVGMKQRLALGGAILHDPPILF
                     LDEPTSGVDPIMRRNFWELIYRFSEAGKTIFVTTHYMDEAEHCDRIALVIDGKIIALD
                     TPALLKENLPYEVYALRVDNFIEVFGLVSGAEGIEEAAIFGSDIHLLCEKGRVGRAGL
                     DRTLRGLGLSGFSLERIPPTLEDVFVTNARRFGL"
     gene            complement(5272..6453)
                     /locus_tag="PKJ16_05365"
     CDS             complement(5272..6453)
                     /locus_tag="PKJ16_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV46450.1"
                     /translation="MMTLKRAVERHLRFGIVRSFIKKEFKQMFRDPRMRMTMFVPPVV
                     MLLLNGYAISTDVEDIRMAVYDADRTRASRELVERFTASGYFIADAALDSEKQIARLL
                     DTGAVELVLHVERGFSRRITSGKKAALQIIVDGTDSSRAAVIVAYVNEITAGFSSAYL
                     DRRVRSLAISKNIQMVKFKPPVELRERSLFNPQLLSRYFFLSGLLGMLVSIVAISMTS
                     MAIVKEREAGTIDQIIVSPIRPAELIAGKIVPYAIVAFIDIVLITAVMVAWFKVPFNG
                     SVLFLFASGILFIVATSAMGLFISTISKTQQQAILSTFLFFIPALLFSGFIFPIYAMP
                     ETIQAITLLNPFRYFITISRAVFLKGAGAIVLWKELAALVIIALVLFYFSARRFRRGL
                     E"
     gene            complement(6455..7606)
                     /locus_tag="PKJ16_05370"
     CDS             complement(6455..7606)
                     /locus_tag="PKJ16_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV46451.1"
                     /translation="MMLSRSIIRIAALAEKEWIQIRRDTRSLILSIFLPVFLLLIFGY
                     ALTMDVKRVSTAVVDQDHSRLSRRFVEKFAHTEYISLYGHVRSTREIDALIDRGDIIM
                     AIVIPRNFERMHQSGKRADIQLVVDGSDSTSATVAIGYVKSILFEFNNENKIRELGAI
                     GIASVAMPVEVRSRIWYNAELKSKNFIVPGIIVIVMSIVSALITSLTISREWERGTME
                     TLITTPVRKFEVFLGKLVPYILIGLFDIILTIAVGFFVFKVPLRGSFIELCLVSLLFL
                     VGTSSIGIMISSATRSQVLSVQIAINLTYLPTIILSGYVFPIKNMPAFVQAITYLIPA
                     RYLISYMKGITMKGIGYTVLWSQILFLFLFALGVALVSLRKLSLRLPEK"
     gene            complement(7603..8526)
                     /locus_tag="PKJ16_05375"
     CDS             complement(7603..8526)
                     /locus_tag="PKJ16_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46452.1"
                     /translation="MISIRNVRKRFGDTEAVAGLSLEIATGEIFGIVGPDGAGKSTLL
                     RMISTILAPNEGAVEIDGVNVFENPYRIKDHLAYMPQRFGLYEDLTVEENLVFFGRLF
                     GVTRREIRSRMKRLYQFSRLEPFRDRLAGNLSGGMKQKLGLACSLIHNPRVILLDEPT
                     NGVDPVSRREFWNILYELLSDGVTIVVSTAYLDEAERCNRIALMFNGGIVVTGRPGEI
                     KRSIGRRFVAFTTSDPRHSEEALREVPEFEGVILTGNTLHVFVDSVAAARRRIVAHLR
                     RKRIRVLSFKETLPGLEDCFVDIVARGGRSA"
     gene            complement(8523..9323)
                     /locus_tag="PKJ16_05380"
     CDS             complement(8523..9323)
                     /locus_tag="PKJ16_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV46453.1"
                     /translation="MKRRTLVPLVIVAAALVASVLYFEVFRHITFDDSRIEGSGTIEV
                     TEVEISTKIAGRVASLPFEEGSAVKRGDLVAKLEFEELSASHHSARANLVNAERNLMR
                     IRDLHGSGSVSRRDLDNAETAYRVAKANVDYVNASILNAVIYAPIDGVVLERNLEAGE
                     MAFPGSAIITLADLKRPWIKIYVPQPKLHRVKLNQKAEVTIDGTKKRFSGKVVAISNK
                     AEFTPKTIQTKDERVKLMFAVKIAVENPAGELKPGMPADAAIIIEGAR"
     gene            complement(9320..10795)
                     /locus_tag="PKJ16_05385"
     CDS             complement(9320..10795)
                     /locus_tag="PKJ16_05385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV46454.1"
                     /translation="METTRKVACALALVVSMCVLGHDPSIEAAQEGPAVPKKITVDEA
                     VAIALKRNHDYVIAVQRRAIAKEKINSVWSQLMPVIESEASLTRQRAENGQMSLSDGQ
                     NDIRFVQLRFGINPGVFYNSLKASRDSYKAACQEVRRLRSEITYNVIKSYFSLLVAEE
                     MIKLRRDSLVYLRENEKDVRNMYRTGSVPRFELLQARVKHKSQEPLLHEAENDYRVAM
                     DLFNYHLGAAPGEYAVQSGILDDDSFRAPQGPMDDTIRRLIDIAMRNRPEIVQLSLKR
                     DIARSAKNMYSSYYLWPTFTLVGGYGRTQFLVNEPAVDPITMQIPTPFGTFGNSYTPD
                     YSPIIGNRNWQETWSVRVAATYRWSSLLPSDQTSAAEREERIKVKEAEEEIVRIKRMI
                     ALSLRASYSRLHTAVMTIASQKENMASAAEGLRIAKESYRAGIIKNAELLSSEVALTN
                     ARTGYINAVHNYYTALAELRREVGSDSDAVIFAAAKQEAAK"
     gene            complement(10797..11414)
                     /locus_tag="PKJ16_05390"
     CDS             complement(10797..11414)
                     /locus_tag="PKJ16_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46455.1"
                     /translation="MEQATTEQKIISAAIREFAHNGFAGARIDRIAKQARINKAMIYY
                     HFRGKEALYERVLFDKFKGIMDSIEESYIGGDDPLAELYASIRNYVKYITSLDADVIR
                     IILREISGGGKYFKKIALPTLIVPIHARVIAIIEDCQRRGLIREVNPHYLFFQTVGAI
                     NFFNIMRLALGDSGLVDFLKNEETVEEYTQTIIDTLRMGVQAKEV"
     gene            complement(11517..12788)
                     /locus_tag="PKJ16_05395"
     CDS             complement(11517..12788)
                     /locus_tag="PKJ16_05395"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV46456.1"
                     /translation="MTVDVAYLPYAVATGACAVLSFFFSGIETAMISSSEITLRSLAD
                     GGSARARRALAIITRMEEALGTTLIGNNIVNIASASFITYIAARGFLLNERELLAVSA
                     VQVSVFLVFCEILPKVLARSLAERFLMFFSMPLLLVIRVLGPLSAPALAFSRALKRRM
                     GITPDRSLRFNLRDEVNYFFTRKTRDEQIGDLERELLGEIMSFHEVTAGEIMIPIIDI
                     VSVERKKSVRQLVGLIDRTNFSRIPVYEERVDNIIGYVYYRDLLQRRDARDFDGLIRR
                     ARYVPRTKRIYQLYREMVEDEESIVFVVNEYGGVEGLVTPEDIAEEIVGEIQTRDHPD
                     QVLISSLGRNRFLLSGSLNIDYFTRRFGVPIDKKGFETIAGFIMYVLGHVPREGEAVS
                     HAGCKFIVDAATEKSIEKVIVVIPAAKKIKK"
     gene            complement(12785..14101)
                     /locus_tag="PKJ16_05400"
     CDS             complement(12785..14101)
                     /locus_tag="PKJ16_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV46457.1"
                     /translation="MSLFAVHVGALVVLLGLSFFFSGSETALFSLRRSDLHRFSSAGD
                     GRNRLIAAIMESPQKILVTILTGNLFVNMIMSEVSTVLFLSVWGDYGHFISIAVLTPV
                     VIIVSEITPKVFAITNPVRFARRVVVLLDFLHRLLWPLRALLLMITDGLVSLFGQGAA
                     GETERRDAELDTIVEMGVSDGMISREEGDFIRNVLRFSRKEAANVMIPRNTAVFISAH
                     ATIDEAAAVFLEHNIPRAPVYDGDYDTIVGILDSRELVPYCMGMRRTKRITSLMYTVY
                     HYPSSIELGELLKDFLVKKLQMAIVLDEYGGTAGVVTLSGILSELMGRAFLQWGEFKR
                     QEIRRVDERTAVVPGDLQIDEFNERFGESIEAEDSDTLAGYIIERLGHVPRRGETLEL
                     ARHVLAVKWVRRNRVERVSVVEKQPQADQSAGFAFRWPWRRVKGDR"
     gene            complement(14147..15034)
                     /locus_tag="PKJ16_05405"
     CDS             complement(14147..15034)
                     /locus_tag="PKJ16_05405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV46458.1"
                     /translation="MLEPLPRATRFIFLFSLILLQLLCASFRWPLVDGRITSTFGESR
                     WDHLHDGIDMIAPTNAVHPIARGRLVYAWDRSLFPLDNYPGGGNVRILEHDGGIYSVY
                     MHCDDSPRIKALYEESEPVAIMGNTGRSLGRHLHFSILMRKERTSVNPFKLLPAWPDG
                     KAPVIGDCAVRIEDRYFPIKNNSNIRLTKHYPLLVEIFDSERGNERLGVYRLIIALNG
                     KVLVEADFTKIGIGPGGLHINGKTYDDVFDQKGYYKALNATYTDGINTVKITAADYAG
                     NVAEKEISFNVKLETPETR"
     gene            complement(15050..17395)
                     /locus_tag="PKJ16_05410"
     CDS             complement(15050..17395)
                     /locus_tag="PKJ16_05410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNV46459.1"
                     /translation="MRQIFDRAVSIALEVKDWVGAHKKIVFIYVPVILFVLFGAYVGV
                     IYGAWRSDRDAALSKLSRYKRLIERTEELKAGTSFNSSEVDLEAKVVDIPTRIYDRND
                     EIIGEFFEQKREIVPFGFIPPILVKAVIASEDRDFRNHRGVSYKGIFRAFVNNLLHLR
                     VVQGGSTITQQLAKVLFTDMDRSIKRKIYETFCAHEIEKRYDKDDITSMYLNLINFGK
                     GVYGVESTAKMYFGKSVNELNDVECAMIVATISNPGLYSPLYNLDNSVKKTHRIMKSL
                     VDAGYVNQARAEYQYKRFLEKWEVAFDEKKRAKSSLIGSFVYSTYRVNRSPFFNEQIR
                     RKLVEKWGEDVVKKGGLTVYTTVDATMQDAALHALRTGVSRQRDYHLRVARRLKDPTR
                     YEQEINKARNVEGALVAIDPYTGEMLAYVGGYEFTSANQFDNASQMQRQPGSSFKPVV
                     YAAAIENRDITPSTVLVDEKTTFRGKYSPRNYDGNYLGKIIAREALAKSINVVAVKVL
                     NKTGYDRIFDMLGNGLDLDGSELTRRFGKTLSLALGTYDVSPLENCVLHSVIVNGGDY
                     IAPYGIRYVKDYNGTIVWNNEEEMKKLIDERRAKMGKLLEPAACAVTVSMLKGVLEDG
                     GTAHYTVKHRKIPFPIAGKTGTTSNYVDAWFVGYTADLVTAVWIGNKQGSVSLGGGRA
                     GASIAAPVWVDFVTKAYRDKTPGDFKTPDEGITRQSICRDSGLVPRGEGTCPRIARDQ
                     IFIAGTEPGEYCTLHGGDKTKIAEGKDGAGTDAPESSKKSN"
     gene            complement(17399..18445)
                     /gene="rlmN"
                     /locus_tag="PKJ16_05415"
     CDS             complement(17399..18445)
                     /gene="rlmN"
                     /locus_tag="PKJ16_05415"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HNV46460.1"
                     /translation="MTCIKDLSLEDAEALFAEWGEKPFRARQLFVWLYEKNVASFAEM
                     TTFSKVLRARLDAEFEINALALEDRLVSAIDGTEKYLFRTSDGNFIESVLIKRDGDDG
                     GRLTECISSQVGCAMGCAFCSTARMGFVRNLTTGEIVDQICQVRRISGLINHNVVFMG
                     MGEPLLNYDAVMKAAAIINYSFGFHVSVRRITISTCGITPAIERYIDERRPYNLAISL
                     NDTLPEARRRNMPVEGAYPIAGISRLLDEKAPASRNRVTLEYVMRKDNIGADDARRLK
                     SLFRASWIKLNLIPLNPGASGAEAPTEVEIARFVTELEIMNVPVTVRKSFGSDIAGAC
                     GQLSGARYHQQIAG"
     gene            complement(18470..>20304)
                     /locus_tag="PKJ16_05420"
     CDS             complement(18470..>20304)
                     /locus_tag="PKJ16_05420"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46461.1"
                     /translation="PAFVIAGIAVGTDWNISRAYIENKVLVREFRFFYTVYAVVTFLY
                     ISVAVAKFVINYLKTKVAIYRLQMRYVFIGTTVSMLIAAVFSIIMPRFFNYSELYVLG
                     PSIASFIAVGSFFYSVISYQMMDITTAIHKTVMYTIISTLIFLPIFGILWAYDARRES
                     LIAVHPMLVAGSLVGVFLFFSVYVQPQVDRIFKRRQYEFENVINNFIIDVEKIRDFRQ
                     LVQRTVDILVDSLFLKNAVFFLFSNETRRYDPLYRRGEEFEAEPVDRNSPLVRWFVRN
                     QEVLYLDRVYTDDRSFGEMREEFAGFFLKNRARLVMPMYHGRRMLGLLCLGDKDSLAG
                     YKPDELVTLQNFQRECNVHLSNALTYEEAMREELVNRTVELSSNIMARSVPENLPNLI
                     GVKFGAFYVPKYKEGVDYFDFLRPGSQGVGVIGTDISGVGITSALNSVVLRSAFQSCV
                     EDAPSAYSVMQKLNRILYDYSEGRAGFITAYYFYYDIKTMRLIYSNAGFPPLDIYRIE
                     KNDFDSLDTEGIPLGYDPEASYGMGRTNLLRGDIGMLYSKALINTKNQRGDEYGLHRL
                     RTVVKENRSGRPFEIANAVKKDFTDFLGLSTPESDIVFIVFKIV"
BASE COUNT         4071 a         6268 c         6008 g         3957 t
ORIGIN      
        1 aagttgtgga cgaacccggc gatgatgcgt atgtcacaat tcgggaatcg acatgttgcc
       61 aaccattatc acatccagcg aactcttcag catgctcttc attttctttg cgcgcatcat
      121 cgatgtgtcg ctggggacca tgcgcatcat tctcatcgcg cgcggctatc gttcactcgc
      181 cccgatattc gggttcctgg aggtgtttat ctggctcggc gccatcggga cgaccatcag
      241 caacctgcac agcattttca gctatttcat ctacgcgggg ggcttcgcgg ccggcaacta
      301 cgtgggcatg ctcatcgagg agcgggtttc catcggctat cagctgatca gaatcatcac
      361 caccaaggag gtcacggcgc tgccgatggt gatgcgcgag gaaggatacg gggtcacggt
      421 cgttgacggc aaggggatgc ggggggacgt gtatatcctc tacgccgtgg tccgtagaag
      481 cgaggtgaaa aaaatcctgg gcatcgtgga aacctttgag ccgagcgcct tcatcacggt
      541 cgaggacgtc cgttcgcaca tcgccgggtt catcggcgac aagggcgggc cgccgttttt
      601 cgggcgcatg ttcgcgaagc gcaagtaggc ggccatccgc tagaccatgt gctcgggcgg
      661 gcgctgccgc gtatcctgct gccggcgcga gaacatgcgg tacccgtagc ccattttgat
      721 gagcaccagg atgagcccga cgagaacggc caacacgccg gcgacgagca atcgattgta
      781 ctccagccga taaaaaatct cgcccacccc gaccttgggc attgccttga gtgtatacgg
      841 caggccgtac tggttcgcga tggtcaacag cgaatcgaga tgaatgacgg gtacacctgc
      901 gcgcgcaaat cggctgatga cgctctcgat ctccatcgcc tttcggctgg gacggagatt
      961 gacgccgggt ttgtaccgca tcttcccgat atacgaacct atgacgaccg tgcccccgcc
     1021 cacgttgatg aacgcatcaa tcgttttatc gccggcccgc tcatagtaca ggtccatccg
     1081 cgagtcgatg ttcgccttga ggtcctcacg ctcgatgatt ggcaacccgt tgcggcgaat
     1141 ggcctcgcgg atcaagcgac gcccctcctc gctctgaccc cgcgcattgt cctgaacgcc
     1201 gccgagcgac gccgccacgc ttcgggactt gatgatgttc ttctgcacca ggagccgctc
     1261 catgtccagc caggtaaagg cggggatgtt cgcgccccag gtcgacgacg agacgctggt
     1321 gatcacgatc atgcgcagct tcatcgcctc gcccgccgcg agcacggcgc aattgagcgc
     1381 gggaaatgat ccggtgaacg acaccgcgac ggtgctgccg gtgcgcacct tcgactcgcg
     1441 gagcatctgc accaccaccg cggcccagtt tggattgagc gtcgcgacct tcgcggcgat
     1501 atcgaccggc gcgctggtaa tcggagaggt ctgtatgccg atgagccccg acgccgtcgg
     1561 gtcgatcttc cggtcgatgg gcggcagatc ctcctgctgc cggtgctcct taatgacgtc
     1621 cattgcgcgc ttcatggtgc gcgccgcatt atacttcatc tcgtaatgcg gttgcttgac
     1681 attcacccgg aacagctcca ccatcgcaag tccgatgatg gcgaccaggg cgatgatgac
     1741 gtgtatctgc caggaaacgg tcgtgggctt ccagaggatc ttcttcattg cccgatctcc
     1801 ctgccgaaga tgagaatgag cgccaaacga acgatgacgc tcgaggtgac cagcgccgtg
     1861 acggtctcga gcacgccctg ccggtccatc cagatggcga tgagccccgg tatgatatat
     1921 ccgacgatcg tgagatccag cggctgcttc ccgatgggga tcaccccgat agagcgcgta
     1981 aaccatccga gcaggaagcc cactaaaatc atgagcaccg tccggcggcg cccatagatg
     2041 atcatgaagg cgctcatcga gtgcacgata aaaaacgtga ggaagctgac cgcgatggtg
     2101 gtcaggatca taatgggctt gttgatgctc agtgcgatat agccgggcac caccatgccg
     2161 ccggcggcga tccccagcat ctcagaaaag agcaggctca ccgcgagccc gagtccgatt
     2221 gacacgatga gaatgttttc catcacccct ccgcggcgcc gccgtacgcc atgaccgttg
     2281 accggttttt aaaatacttg atgagctcga ggccgggacc ggcgatattc cccaccccca
     2341 tgacgagggt cgattcgcgg caatggctca ggatctcctc gaaaatgctc tccgcgtcaa
     2401 ggccctcggc attgacgaac actcgcggat cgataccgga ccggaccgcg tatttaacca
     2461 gaaaataaga ccccgatccg atgagcacat acttgtcggc cgccggccat gacgcgagcg
     2521 cctcaccgaa ctgccgtgac cggtccggac ggtcgatgcg ggtgttgatg accatgatgc
     2581 gtcccttcgc cgccgaatgc gacttgagcg ccatgcgcca gatccgttcg aaggactccg
     2641 ggtcgttcgc ggcaaacccg tttataaaac agatatcgcg accgaagaaa ttcatgcgca
     2701 ggtcgctcat cgctcccgga tccggcgcga ccgactgcat cccggcaagc gctacgtcgc
     2761 gctccacccc gatcgcctcg cacacgctga gcgccaatgc gacattctcg ggatgctcca
     2821 aataggagaa ccgcgccata tcggacgctt caagcgcccg gaccgcgtcc tcacccacga
     2881 atacgcatcg cgtattccgc tgggcgcaca cggcggcgaa ctcatcggga taatcttgct
     2941 cggccgtaaa caggaccgcg tcgttgggcg tggtgccgag gatcgcgagg gcgacatccc
     3001 gctcggtggg ccccatgacg tcgagatggt cctcgcgaac gttcgtgatc accccgtgcg
     3061 tggcgcggat catcttttgt tccgagaggg attggaggtg cggctgcagc gccatgcact
     3121 cgatcaccag cgcgcgggcg cggttccgcg ccgcgaaggc gacgatacgc agctgttcga
     3181 tgatattcgg gcgcgattgt cggaacacgg ggtattccgt gccatcatcg acgatgacgc
     3241 gcggcagggt cccggtcgtc ttggcgattg tggtaatccc gcccgcgcgc aagccggcgg
     3301 cgataagccg ggtcaccgag gacttacccc gggtgccatt gacatgaatg cggatcggga
     3361 tggcccgaag atttcgccga tgaagagaat actcgacaat gccgagcccg accaggacac
     3421 cgatcaggag cacgagaata gttattccgg tcataccgtc cccatctgag tgcgacacat
     3481 ccgtttgggg accgggggcc ccatttttca acactttttt ccagctgcca accaccgccg
     3541 cccccctctc cggaggcggc gaccagaaaa tgagttgcct tcccgttagc aaccggcgat
     3601 atggtacgtg cgaaaaaata tattattgac ttgatgtcgt tctcacgcga ccccgcgccg
     3661 ctcaattcac agccgggttc tgcgccgcag accacgggaa atctgcgaac cgcatggacc
     3721 ctgggaccgt ctgaacgatt gacaacgccc gtacgggcag cgaaagcggg gtaccactat
     3781 ggacatcggg cccatactga agaaaatccg cctcgagcgg cactataccc tgagccaggt
     3841 cgcgggcaag attggttcca ccgcaagcct cctttcccag atcgaaaact gcaagatatc
     3901 gccgtcgctg cattcgctcg agtcgctgct caaatgctat tcggtgagcc tctcggactt
     3961 cttccggcag gtggaacaga agaactacat cttcctgaaa cggtccgaat ccgaaacatt
     4021 cagcaatccc gacgaaggct accgcctcac cctgctcgcg tcaaagctgc aaaacaacac
     4081 cctcgaatcg tacattgtcg agatagaccc cgaccgcggg atcagcctcg ccacgaccgg
     4141 caaggaactc aatggagagc ggctcatcct tatcttttcc gggcaggcgc aggtcgaaat
     4201 gtcggacgaa gtgatcttgg ccatggccga aggcgactct gcaaacttca agtcgtacgt
     4261 gccctgcacc atccgcaata ccggcgccga cgtggcgcgt atgctcatca gcgggatgcc
     4321 gcccatcata ctgtagtcgc cgctacagcc cgaaccggcg agcattggtc acaaagacgt
     4381 cctccagcgt gggcggtatg cgctcgagcg aaaatcccga caacccgagt ccgcgaagcg
     4441 tccggtcgag acctgcgcgc cccacgcgtc ccttctcgca gaggagatgg atgtcgctcc
     4501 cgaagatggc agcctcctcg atgccctcgg cgcccgaaac cagaccaaag acctctatga
     4561 agttatcgac gcgcagtgcg tacacctcgt agggaaggtt ttccttcaaa agcgccggcg
     4621 tatcgagggc gatgattttc ccgtcgatga caagcgcgat ccggtcgcag tgctcggctt
     4681 cgtccatgta gtgtgtggtc acgaaaatgg tcttccccgc ctccgaaaac cgatagatga
     4741 gctcccagaa atttcggcgc ataatcgggt caacgcccga ggtgggctcg tcgagaaaga
     4801 gtatgggcgg atcgtgcagg atcgcgcccc ccagcgcgag acgctgcttc atgccgacgg
     4861 gcaggctccc gacgatcgca tccttccggc cggcgatctc cgccatctcg agggcgtacg
     4921 ccatccgctc agcgaggaga gcgccggaaa gcccgtagat gctgccgaaa aactccaggt
     4981 tctcgtacac cgtgagatcg ccgtagagcg agaacttctg cgacatgtag ccgatccgcg
     5041 ttttgatggc gtcctggtcg gcgaggatgt cgaagtcgag cacgcgcccc gttccggacg
     5101 agggggcgat gattccgcag agcatcttga tggtggtgct cttgcccgcg ccgttggggc
     5161 ccagaaaccc gaagatctcg cccgggcgca cctcgaatga tatgtcgttt accgcggtga
     5221 acgcgccgaa gtttttcgta agatgttgca cggcgacgct gggttgtccg ctcattccaa
     5281 tcccctgcga aaacggcgcg cgctgaagta aaagaggacg agcgcgatga tgacgagcgc
     5341 cgcgagctcc ttccacagga cgatggcgcc ggcgcccttc aggaacaccg cgcgcgatat
     5401 ggtgataaaa taccggaacg gattcaggag cgtgatcgcc tgtatcgtct cgggcatcgc
     5461 gtatatcgga aatatgaatc cggaaaaaag caacgccggt atgaaaaaga ggaaggtcga
     5521 aaggatcgcc tgctgttgcg tcttggatat ggtcgagatg aacagaccca tcgccgaggt
     5581 ggccacgata aacaggattc cgctcgcgaa taaaaagagc acgctcccgt tgaagggcac
     5641 cttgaaccag gcgaccatca cggccgtgat aagcacgatg tcgatgaatg cgacgatggc
     5701 gtacgggacg atcttccccg cgatgagctc ggccggtcgg atcggcgata cgatgatctg
     5761 gtcgatggtc cccgcctcgc gctccttcac gatggccatc gaggtcatgc tgatcgcgac
     5821 gatggacacg agcatgccca gcagccccga aaggaaaaaa taccggctca acagttgcgg
     5881 attgaacagg gatcgctccc gaagctcgac cggcggcttg aatttgacca tctggatgtt
     5941 cttgctgatc gcaagcgagc gcacgcgccg gtccaggtag gccgaggaga agcccgccgt
     6001 aatctcgttc acatacgcca cgatgaccgc ggcgcggctg gaatcggtcc cgtccacgat
     6061 gatctggagc gcggccttct tcccggaggt tatgcgccgc gaaaagccgc gctccacatg
     6121 gagcacgagc tcgaccgcgc cggtatccag cagacgcgcg atctgcttct cggaatcgag
     6181 tgcggcgtcc gcgatgaagt accccgaggc ggtgaagcgc tccaccagct cgcggctcgc
     6241 gcgggtgcgg tcggcgtcat agaccgccat gcggatgtcc tccacgtcgg tgctgatcgc
     6301 gtacccgttc aggagaagca tcacgaccgg cggcacgaac atggtcatcc gcatccgggg
     6361 atcgcggaac atctgcttga attccttctt gatgaagctc cggacgatcc cgaagcgcag
     6421 gtggcgttcc acggcgcgtt tcagcgtcat catcctattt ctccgggagc cgcagcgaca
     6481 gttttcgcag gctcacgagc gccacgccga gcgcgaacag aaaaaggaac aggatctgcg
     6541 accagaggac cgtgtagccg atccctttca tcgtaatccc cttcatatag gagatgagat
     6601 agcgcgccgg gatgagatag gtgatcgcct gcacgaaggc cggcatgttc ttgatcggga
     6661 agacataccc ggagaggatg atcgtgggca ggtaggtcag gttgatggcg atctgcacgg
     6721 agagcacctg cgagcgcgtg gccgatgaga tcatgatccc gatgctcgag gtgcccacca
     6781 gaaacagcag cgacaccagg cacagctcaa tgaaactgcc gcgcaggggc accttgaaga
     6841 cgaagaaccc gacggcgatg gtcaggatga tgtcgaacag cccgatcagg atatagggga
     6901 cgagctttcc gaggaacacc tcgaacttcc ggacgggcgt ggtgatgagc gtctccatcg
     6961 ttccccgctc ccactcgcgc gagatcgtca gcgacgtgat gagcgcggac acgatcgaca
     7021 tgacgatgac gatgatgccc ggcacgatga agttcttgct cttgagctcc gcattgtacc
     7081 agatccggct gcggacctcc accggcatgg cgacggaggc gatcccgatc gcgcccagct
     7141 ctcggatctt gttctcgttg ttgaattcga agagaatgga cttcacgtag ccgatcgcga
     7201 cggtcgccga ggtggaatct gagccgtcca cgacgagctg gatgtcggcg cgctttccgg
     7261 actggtgcat ccgctcgaaa ttccggggta tcacgatggc catgatgatg tcgccccggt
     7321 cgatgagcgc gtcaatttca cgcgtggagc gcacatgccc gtagagcgat atgtactcgg
     7381 tgtgggcgaa cttctcgaca aagcggcgcg agaggcggct gtggtcctgg tcgacaacgg
     7441 cggtgctcac gcgcttcacg tccatcgtga gggcgtaccc gaagatgagg agcaggaaga
     7501 ccgggagaaa aatcgagagg atgaggctgc gggtgtcgcg ccggatctgg atccactcct
     7561 tttcggcaag cgccgcaatg cgtatgatcg agcgggacag catcatgcgc tccgccctcc
     7621 ccgggccacg atgtcgacga agcagtcttc caggcccggg agcgtttcct tgaatgagag
     7681 cacgcggatg cgcttacgcc tcagatgggc gacgatccgc cgccgcgccg cggcgacgga
     7741 atcgacaaac acatgcaacg tgttcccggt gagaatgacg ccctcgaact cggggacctc
     7801 ccgaagcgcc tcctcgctgt ggcggggatc gctcgtggta aacgcgacga agcgtcggcc
     7861 gatcgagcgc ttgatttctc cgggccgccc ggtgacgacg atgccgccgt tgaacatcaa
     7921 cgcgatgcgg ttgcaacgct cggcctcgtc gagataggcc gtgctcacta cgatggtcac
     7981 gccgtccgac agcagctcgt agaggatgtt ccagaactcg cggcgggaca cgggatcgac
     8041 gccgttcgtc ggctcgtcga gcaggatcac gcgtggatta tggatgaggc tgcaggccag
     8101 gccgagcttc tgcttcatgc cgcccgagag gttcccggca agccggtctc ggaacggctc
     8161 gagtctgctg aactgataca ggcgcttcat gcgcgagcgg atctcccggc gcgtcacgcc
     8221 gaaaagccga ccgaagaaca ccaggttctc ctcgacggtg aggtcctcgt acagaccgaa
     8281 gcgctgcggc atgtaggcga gatggtcctt gatgcggtag ggattctcga agacgttcac
     8341 cccgtcgatc tcgacggcgc cctcgtttgg cgcgaggatc gtggatatca tgcgaagcag
     8401 cgtgctcttc ccggcgccgt cgggtcccac gatgccgaag atctcgcccg tcgcgatctc
     8461 caggctcaat cccgcgacgg cctcagtgtc gccgaaacgc ttgcgcacgt ttcgaatcga
     8521 gatcaccgcg cgccctcgat gatgatggcc gcgtcggccg gcatgcccgg cttgagctcc
     8581 cccgcggggt tctcgacggc gatcttgacc gcgaacatga gcttcacgcg ctcgtccttg
     8641 gtctggatgg tcttgggcgt gaactccgcc ttgttcgaga tcgcgaccac cttaccggaa
     8701 aaacgttttt tggtgccgtc aatcgtcacc tcggcctttt ggttgagctt cacccgatgg
     8761 agtttcggct gcggcacata gatttttatc catggccgtt tcaggtcggc gagggtgatg
     8821 atggccgagc ccggaaacgc catctcgccc gcctcgaggt tccgctccag caccacgccg
     8881 tcgatgggag cgtagatcac ggcgttgagg atggacgcat tcacgtagtc gacgttcgcc
     8941 ttggccacgc gataggccgt ctcggcattg tccaggtcgc ggcgcgagac cgatccgctc
     9001 ccgtgcaggt cgcggatgcg catgaggttc cgctcggcgt tcaccaggtt cgcccgggcg
     9061 gagtgatggc tcgcggaaag ctcctcgaac tcgagcttgg cgaccagatc gccgcgcttg
     9121 acggcgctcc cctcctcgaa cgggaggctg gccacgcggc cggcgatctt cgtgctaatc
     9181 tcgacctcgg tcacctcgat ggtcccggat ccttcaatgc gcgagtcatc gaacgtgata
     9241 tgccggaaga cctcgaaata gagcacggag gcgacgagcg ccgctgcgac gatgacgagg
     9301 ggaacgagtg tacggcgttt catttggctg cctcctgttt cgcggcggcg aagataacgg
     9361 cgtcggaatc gctgcccacc tcgcggcgga gctcggcgag cgcggtgtag taattgtgca
     9421 cggcattgat gtaaccggtc cgggcgttcg tgagcgccac ctcggaggaa agaagctcgg
     9481 cgttcttgat gatccccgcg cgatagctct ccttggctat gcgcagtccc tccgccgcgc
     9541 tggccatgtt ctccttctgc gatgcgatgg tcatcaccgc ggtgtgcaag cgcgaatagc
     9601 tcgcgcgaag cgagagggcg atcatccgct tgatgcgcac gatctcctcc tctgcctcct
     9661 tcaccttgat gcgctcctcg cgctcggccg cgctcgtctg gtccgacggc agaagcgagc
     9721 tccagcgata ggtggccgcc acgcgcaccg accaggtctc ctgccagttg cggtttccga
     9781 tgatggggga ataatcgggc gtataggaat tcccgaaggt gccgaatggc gtcggaatct
     9841 gcatggtgat cggatcgacc gcaggctcgt tgaccagaaa ctgcgttctc ccgtatcccc
     9901 ccaccagcgt gaaggtgggc cagagataat acgagctata catgtttttc gcgcttcggg
     9961 cgatatcgcg cttcagggaa agctgcacga tctccggccg atttcgcatg gcgatgtcga
    10021 tcaggcggcg tatggtatcg tccatggggc cctgtggcgc ccggaaggaa tcatcatcta
    10081 aaatcccgct ttgaacggca tattcccccg gcgcggcccc cagatgatag ttgaacaggt
    10141 ccatggcaac gcggtagtcg ttctccgcct catggagaag cggctcctgg ctcttgtgct
    10201 tcacgcgcgc ctgtaggagc tcgaatcgcg gcacgctccc ggtccggtac atgtttcgca
    10261 cgtccttctc gttctcgcga agatagacga gcgaatcccg ccggagcttt atcatctcct
    10321 cggccacaag cagcgaaaaa tagcttttaa tcacgttgta ggtgatttcg ctgcgcaatc
    10381 gccgcacctc ctggcaggcc gccttgtagg aatctcgcga cgccttcagg gaattataga
    10441 acacccccgg gttgatcccg aagcgcagct ggacgaaccg gatgtcgttt tgcccgtcgg
    10501 acaggctcat ctgcccgttc tccgcgcgct ggcgggtgag cgaggcctcg gactcgatca
    10561 ccggcatgag ctggctccag acgctgttga tcttctcctt ggcgatcgcg cgtcgctgca
    10621 ccgcgatcac atagtcgtgg ttgcgcttga gggcgatggc gaccgcctcg tccaccgtga
    10681 tttttttcgg cacggccggc ccctcctggg ccgcctcaat cgaaggatca tgaccaagaa
    10741 cgcacatcga cacaaccagc gcgagcgcac atgcgacctt ccgcgtcgtt tccatatcag
    10801 acctccttcg cctgcacgcc catgcgcaag gtatcgatga tcgtttgcgt gtactcctcg
    10861 accgtttcct cgtttttcaa aaaatcgacg agtccggaat ctccaagggc gagtcgcatg
    10921 atattgaaaa aattgatggc gcccaccgtt tgaaagaaca ggtagtgggg attcacctcg
    10981 cggatgagtc cgcggcgctg gcaatcctcg atgatggcga tgacccgcgc gtggatcggc
    11041 acgatgagcg tcggcagggc gatcttcttg aagtacttcc cgccgcccga tatctcgcgg
    11101 aggatgatcc ggatcacgtc cgcgtccagc gaggtgatgt atttcacgta atttcgaatg
    11161 ctggcataga gctccgccag aggatcatcg cctccgatat agctttcctc gatggaatcc
    11221 atgatgccct tgaatttatc gaacaacacg cgctcgtaga gcgcctcctt tccgcggaag
    11281 tggtaataga tcatcgcctt gttgattcgc gcctgcttcg cgatacggtc gatacgcgcg
    11341 ccggcgaaac cgttgtgcgc aaactcgcgg attgcagcag aaattatttt ttgttcagtt
    11401 gtggcctgct ccattcccaa caccatcaat caaggcactt tttaaccaac catttggttt
    11461 aactaactat ttagttatat gtatgatatg agagggtttt cgtcaagcca taatgcctat
    11521 ttttttatct tttttgccgc agggatcaca acgatgacct tctcgattga tttctccgtc
    11581 gccgcatcga cgatgaactt gcagccggca tggctcaccg cctccccctc tcgcgggaca
    11641 tggccaagaa catacatgat aaagccggcg attgtctcga accccttctt gtcaatcggg
    11701 accccgaacc ggcgcgtaaa gtaatcgata ttgaggcttc cgctcagcaa aaatcggttt
    11761 cgccccagcg acgagatgag cacctggtcg ggatggtcgc gcgtctggat ctcgcccacg
    11821 atctcctcgg cgatgtcctc gggagtgacc agcccctcga ccccgccata ctcgttcacg
    11881 acaaaaacga tcgattcctc atcttcgacc atttcgcggt acagctggta gatgcgcttg
    11941 gtccgcggga cgtatcgtgc gcgccgtatc agcccatcga aatcccgagc gtcccgacgc
    12001 tgaagcaggt ctcgatagta cacatacccg atgatattgt ccacgcgttc ctcgtagacg
    12061 gggatgcggg aaaaattcgt ccggtcgatg agccccacca gctgccggac gctttttttc
    12121 cgttcaacgg acacgatgtc gatgatcggt atcatgatct ccccggccgt cacctcatga
    12181 aagctcatga tctcgcccag aagctcgcgc tccagatcgc cgatctgttc gtcccttgtt
    12241 ttacgcgtga agaaataatt cacctcgtcg cggaggttaa agcgcagcga cctatccggg
    12301 gtgatcccca tacggcgttt gagcgcccgt gaaaacgcga gcgccggcgc gctcaacggc
    12361 ccgagcaccc tgataacgag aagcagcggc atcgaaaaga acatcaggaa gcgctcggcg
    12421 agggatcgcg ccaacacctt gggcagtatc tcgcagaaca ccaaaaagac cgagacctgg
    12481 accgcggaga ccgcaagcag ctcgcgctcg ttgagcagaa acccgcgggc cgcaatatac
    12541 gtaataaacg acgccgaggc gatattgacg atattgttcc cgatgagcgt ggtccccagc
    12601 gcctcctcca tccgggtgat gatggccaac gcccgacgcg cgcgagcgct tccgccgtcg
    12661 gcgagcgatc gcagcgtgat ctcgctggag gagatcatcg ccgtctcgat cccggaaaag
    12721 aaaaacgaga gcacggcgca cgctcccgtc gcgaccgcgt acggcagata ggcgacatcc
    12781 acggtcatcg gtcccccttc acgcgacgcc agggccagcg aaaagcgaat cccgcagact
    12841 gatccgcctg gggctgtttc tcgaccaccg aaacgcgctc gacccggttt cgccggaccc
    12901 acttcaccgc aagtacatgg cgcgcgagct ccagagtttc gccgcgccgg ggaacgtgac
    12961 ccaggcgctc gatgatatat ccggcaagcg tgtccgaatc ctcggcctcg atggactccc
    13021 cgaagcgttc gttaaactcg tcgatctgga ggtcgcccgg caccacggcg gtgcgttcgt
    13081 ccacccggcg tatttcctgg cgtttgaact ctccccactg caggaatgcg cggcccatga
    13141 gctccgagag gatccccgag agggtcacca cgcccgcggt gccgccgtat tcgtccagca
    13201 cgatggccat ctggagcttc ttcaccagga agtccttgag gagctcgccg agctcaatcg
    13261 acgaggggta atgatacacc gtgtacatca gcgaggtgat ccgcttcgtt ctgcgcatgc
    13321 ccatgcaata gggcaccagc tcgcgcgaat cgagaatgcc gacgatggtg tcgtaatcgc
    13381 cgtcgtacac cggcgcgcgc ggaatattgt gctcgaggaa gacggcggcg gcctcgtcga
    13441 tcgtcgcgtg tgcggagatg aacaccgcgg tgttccgcgg aatcatcaca ttggccgcct
    13501 ccttgcggga gaagcggagg acgttcctga tgaaatcgcc ctcctcgcgc gatatcatcc
    13561 cgtcggacac gcccatttcg acgatggtgt ccagctccgc atcgcgccgt tccgtttctc
    13621 ccgccgcccc ctgcccgaac agcgacacga ggccgtcggt gatcatcagc aagagcgcac
    13681 ggagcggcca gaggagccgg tgcaggaaat cgagcaggac caccacccgc cgggcgaagc
    13741 gcacggggtt ggtgatcgca aacaccttcg gcgtgatctc ggagacgatg atgacgaccg
    13801 gcgtcaacac cgcgatcgag atgaaatgcc catagtcgcc ccagacggac agaaagagga
    13861 cggtggacac ctcagacatg atcatgttca cgaacaggtt gccggtgagg atggtgacga
    13921 ggatcttctg cggcgactcc atgatcgccg cgatgagtcg atttctgccg tcgcccgcgc
    13981 ttgagaagcg gtgcagatcg gaacgcctga gcgagaagag cgccgtctcg gatccggaga
    14041 aaaagaatga aagccccaga agcacgacga gcgcccccac atggacggcg aacagactca
    14101 tggcgcctgt gctgccgcgc gtttcacgac cgggaccgtc cgcccatcag cgcgtctccg
    14161 gggtttcgag cttcacgttg aaggagatct ctttttcagc gacgttgccg gcatagtcag
    14221 cggccgttat cttcaccgtg ttgattccgt ccgtgtaggt cgcgttcaac gctttatagt
    14281 accccttctg gtcgaatacg tcgtcgtatg ttttaccatt gatatgcaat ccgcccgggc
    14341 cgatgccgat cttcgtaaag tccgcctcca ccagcacctt gccgttcagg gcgatgatca
    14401 accgatacac gccgagacgc tcgtttccgc gctcggaatc gaatatctcg accagcagcg
    14461 gatagtgctt cgtgagccgg atgttcgaat tgttcttgat cgggaagtat cggtcctcga
    14521 tgcgcacggc gcaatcgccg atcaccgggg ccttgccgtc cggccatgcc ggcaggagct
    14581 tgaacgggtt cacggacgtc cgctccttcc gcatcaggat ggagaaatgg agatggcggc
    14641 ccagcgagcg gcccgtattg cccatgatgg cgacgggttc gctctcctca tagagcgcct
    14701 tgatccgggg actgtcgtca cagtgcatgt acaccgagta gatgccgccg tcgtgctcca
    14761 ggatacgcac gttccctcct cccggatagt tgtccagcgg aaagagcgaa cggtcccaag
    14821 cgtacaccaa gcggccgcgg gcgatcggat gcaccgcatt ggtcggggcg atcatgtcga
    14881 tcccgtcgtg gagatggtcc cagcgtgact cgccgaaggt cgaggtgatg cgaccgtcca
    14941 cgagcggcca acggaacgat gcgcagagaa gctgcaacag gatcaggctg aaaagaaaga
    15001 tgaatcgcgt cgcgcgcggc agcggttcga gcatcaccgg gcacccccat cagtttgatt
    15061 tcttcgatga ttccggcgca tccgttcctg ccccgtcctt gccctcggcg atcttcgttt
    15121 tgtcgccgcc gtgcagcgtg cagtattctc ccggttcggt gcccgcgatg aagatctggt
    15181 cgcgcgcgat ccgggggcag gtcccctccc cgcgcggcac caggccgctg tcacggcaga
    15241 ttgactgccg ggtgatgccc tcgtccggcg ttttaaaatc gcccggcgtc ttgtcgcggt
    15301 acgccttcgt gacgaaatcg acccacaccg gcgcggcgat ggacgccccg gcgcggcccc
    15361 cgccgagcga aaccgagccc tgcttgttgc cgatccacac cgcggtcacc aggtcggccg
    15421 tgtagccgac gaaccacgcg tccacgtagt tcgacgtggt cccggtcttt cccgcgatgg
    15481 ggaacggtat cttccggtgc tttacggtgt agtgcgccgt gccgccgtcc tcgaggacgc
    15541 ccttgagcat ggagacggtg accgcgcacg cggcgggctc gagcagtttg cccattttcg
    15601 cccggcgctc gtcgatgagc tttttcatct cctcttcgtt gttccacacg atggtgccgt
    15661 tgtagtcctt cacgtagcgg atgccgtagg gcgcgatata atcgcctccg ttgacgatga
    15721 cggagtgcag cacgcaattc tccagcggcg agacatcgta ggtccccagg gcgagcgaga
    15781 gcgttttccc gaaccggcgg gtaagctcgg acccgtcgag gtccagcccg tttcccagca
    15841 tgtcgaagat ccggtcgtag ccggttttat tgagcacctt cacggccacc acgttgatcg
    15901 atttcgcgag cgcctctcgg gcgatgatct tcccgagata attgccgtcg tagttccgcg
    15961 gcgagtactt gccgcggaag gtcgtctttt cgtccaccaa caccgtcgag ggcgtgatat
    16021 cgcggttctc gatcgccgcg gcgtacacga ccggcttgaa cgatgagccc ggctggcgct
    16081 gcatctgcga ggcattgtcg aactggttgg ccgaggtgaa ttcgtaaccg ccgacgtatg
    16141 cgagcatctc gcccgtatac ggatcgatgg ccacgagcgc cccttccacg ttccgggcct
    16201 tgttgatttc ctgctcgtag cgggtcgggt ccttcaaccg gcgcgccacc cgaagatgat
    16261 agtcgcgctg ccgggacacg cccgtccgaa gcgcgtggag cgcggcgtcc tgcatggtgg
    16321 cgtccaccgt cgtgtacacg gtgaggccgc ccttcttgac cacgtcctcg ccccatttct
    16381 cgacgagctt ccgccggatc tgctcgttga agaacggcga gcggttcacg cgataggtgc
    16441 tgtacacgaa gctgccgatg agcgagctct tcgcgcgctt cttctcgtcg aaggcgacct
    16501 cccatttttc gaggaagcgc ttgtactggt actcggcgcg cgcctggttg acatacccgg
    16561 cgtccaccag cgacttcatg atgcgatggg tttttttcac ggagttgtcg agattgtaca
    16621 acggcgaata cagccccgga ttggagatgg tcgcgacgat catggcgcat tccacgtcat
    16681 tgagctcgtt cacggatttc ccgaagtaca tcttcgccgt cgactcgacc ccgtagaccc
    16741 ccttcccgaa atttatcagg ttgaggtaca tcgacgtgat gtcgtccttg tcgtagcgct
    16801 tctcgatctc gtgggcgcag aaggtctcgt atatcttccg cttgatgctg cggtccatgt
    16861 cggtaaagag caccttcgcc agctgttggg tgatggtgct gcccccctgc acaacgcgca
    16921 ggtgcaggag attgttcaca aaggcgcgga agatgccctt gtaactcacg ccgcgatggt
    16981 tccggaaatc gcggtcctcg ctcgcgatca ccgccttcac gaggatgggc ggaatgaagc
    17041 cgaacggcac gatctcgcgc ttctgctcga agaactcgcc gatgatctcg tcgttgcggt
    17101 cgtagatgcg cgtggggatg tccaccacct tcgcctcgag gtccacctcg ctggaattga
    17161 acgaggtgcc ggccttgagc tcctcggtcc gctcgatgag ccgcttgtac cggctgagct
    17221 tcgacagcgc ggcgtcgcgg tcgctccgcc acgctccgta gatgacgccc acataggcgc
    17281 cgaagagcac gaagagtatc accggcacat agatgaaaac gatcttcttg tgagcgccca
    17341 cccagtcctt cacctccaac gcaattgaca ccgcacgatc gaatatctgt cgcattccct
    17401 aacccgcaat ttgttgatga taccgcgccc cggagagctg cccgcaggcg cccgcgatat
    17461 cggacccgaa actcttgcgc accgtaacgg ggacgttcat gatctccagc tccgtgacga
    17521 agcgcgcgat ctcaacttcc gtcggcgcct ccgcacccga cgctcccggg ttgagcggga
    17581 tcagattgag cttgatccac gatgcgcgaa agagcgactt gagccggcgc gcatcatcgg
    17641 cgccgatatt gtccttccgc atcacatatt cgagcgtcac gcggttacgc gacgccggcg
    17701 ccttttcgtc cagcagacgg ctgatcccgg cgatcggata cgcgccctcc accggcatgt
    17761 tccgccggcg cgcctcgggc agcgtatcgt tcagggatat cgcgagattg tagggacgcc
    17821 gctcgtcgat gtatcgctcg atcgccggcg tgatgccgca ggtcgatatc gtgatcctgc
    17881 gcaccgacac gtgaaaaccg aagctgtagt tgatgatcgc ggccgccttc atcaccgcat
    17941 cgtaattcaa gagcggctcg cccatcccca tgaacaccac attgtggttg atgagtcccg
    18001 agatgcggcg cacctggcag atctggtcca cgatctcccc ggttgtcagg ttccgcacaa
    18061 aacccatccg cgccgtgctg cagaacgcgc agcccatcgc gcaccccacc tggctcgaaa
    18121 tgcactcggt gagccggccg ccgtcatcac cgtcgcgctt gatgaggacg gactcgatga
    18181 aattcccgtc gctggttcga aagagatact tctcggttcc gtcgatggcg gagacgagcc
    18241 ggtcctcgag cgcgagcgca ttaatttcaa actccgcgtc gagccgcgcg cgcaggacct
    18301 tcgagaaggt tgtcatctcc gcgaaagagg cgacgttctt ctcgtacagc cacacgaaga
    18361 gctggcgcgc gcgaaagggc ttttcgcccc actcggcgaa cagcgcctcc gcatcctcga
    18421 gagataggtc cttgatgcac gtcatgtccg ggaaccccgc gaagccgccc tacacgattt
    18481 taaacacgat gaacacgata tcggactcag gggtagagag accgagaaaa tccgtaaagt
    18541 cttttttaac cgcgttcgcg atttcaaacg ggcgccccga ccggttttcc ttcaccacgg
    18601 tgcgcaggcg atggagcccg tactcgtccc cccgctggtt tttcgtgttg atgagcgcct
    18661 tcgagtacag catgccgatg tccccgcgca acaggttggt ccgacccatg ccgtaactcg
    18721 cctccggatc gtaacccagg ggaatgccct cggtatcgag cgaatcgaaa tcgtttttct
    18781 cgatccgata gatgtccagc ggcggaaacc ccgcattgga atagatgagc cgcatcgtct
    18841 tgatatcgta ataaaaataa taggcggtga tgaaaccggc gcgcccctcg gagtaatcgt
    18901 agaggatgcg gttgagcttc tgcatcaccg aataggccga tggcgcgtcc tcgacgcatg
    18961 attggaacgc cgagcgcagc accaccgagt tgagcgcgct cgtgatcccc accccggaga
    19021 tgtcggtgcc gatgacccca accccctggc ttccgggccg caggaaatcg aagtagtcga
    19081 cgccctcctt gtacttggga acatagaacg cgccgaactt gacgccaatg agattgggga
    19141 ggttctccgg aacggagcgc gccatgatat tcgacgagag ctcgaccgta cggttcacca
    19201 gctcctctcg cattgcttct tcatacgtaa gcgcgttcga gaggtgcacg ttgcactcgc
    19261 gctggaagtt ctgcagcgta accagctcgt cgggcttgta gccggccagc gagtccttat
    19321 cgcccaggca caggaggccc agcatccgcc ggccgtgata catcggcatc accagccggg
    19381 cgcggttctt caagaaaaag cccgcgaact cctcacgcat ttcgccgaag gagcggtcgt
    19441 ccgtatacac gcggtccaga taaagcacct cctggttccg cacaaaccat cgcacgagcg
    19501 gcgagttgcg gtccaccggc tcggcctcga actcctcccc gcgccggtag aggggatcgt
    19561 agcgcctcgt ctcgttcgag aaaagaaaaa agaccgcgtt tttcaggaag agcgaatcga
    19621 ccaggatgtc cacggtgcgc tgcaccagct gccggaaatc gcggatcttc tccacgtcga
    19681 tgatgaagtt gttgatgacg ttctcgaact cgtactgccg ccgcttgaag atgcggtcca
    19741 cctgcggctg cacgtatacg gagaaaaaga gaaacacacc caccagcgat ccggcaacca
    19801 gcatgggatg cacggcgatg agcgactcgc gccgggcgtc gtaggcccac aggataccga
    19861 aaatcgggag gaagatgagc gtggagatga tggtgtacat caccgtcttg tgtatggcgg
    19921 tggtgatgtc catcatctgg tacgagatca ccgagtaaaa gaacgaaccc accgcaatga
    19981 atgacgcgat cgacggcccg agcacataga gctccgaata gttgaagaaa cgcggcatga
    20041 tgatggagaa caccgcggcg atcagcatgg acaccgtggt gccgatgaaa acgtagcgca
    20101 tctgcaggcg gtagatggcg accttcgtct tcaggtagtt gatgacgaac ttcgcgaccg
    20161 ccacgctgat atacaggaag gtgacgacgg cgtacaccgt atagaagaaa cggaactccc
    20221 gcaccagcac cttgttctcg atataggccc gcgatatgtt ccagtcggtc cccacggcga
    20281 tcccggcgat cacgaaggcc ggca
//
LOCUS       DAORHF010000040        19860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1876_length_19860_cov_6.024590, whole genome shotgun sequence.
ACCESSION   DAORHF010000040 DAORHF010000000
VERSION     DAORHF010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19860
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1876_length_19860_cov_6.024590"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..199)
                     /gene="secE"
                     /locus_tag="PKJ16_05425"
     CDS             complement(14..199)
                     /gene="secE"
                     /locus_tag="PKJ16_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HNV46462.1"
                     /translation="MFKKVIQFVRESRDELKRVTWPDRDEVTSFTMIVIVAVVIVSLF
                     LYGVDTVLGMLVKGLLD"
     gene            complement(230..382)
                     /gene="rpmG"
                     /locus_tag="PKJ16_05430"
     CDS             complement(230..382)
                     /gene="rpmG"
                     /locus_tag="PKJ16_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HNV46463.1"
                     /translation="MRDIILLSCKDCKRKNYSLTKNKKTQTGKLEVMKYCKFCNKHTL
                     HKETKA"
     gene            complement(777..849)
                     /locus_tag="PKJ16_05435"
     tRNA            complement(777..849)
                     /locus_tag="PKJ16_05435"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(814..816),aa:Thr,seq:ggt)
     gene            complement(1003..2007)
                     /locus_tag="PKJ16_05440"
     CDS             complement(1003..2007)
                     /locus_tag="PKJ16_05440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HNV46464.1"
                     /translation="MTKRIFGTILVVVFVAAMLSASSCAPRHARTGAGDRAVAAGDSD
                     DFSYDENDRDYDALDRNAAGKKQQWAERDAEANEGVRPVRNASRSRSIDRDEERSFDD
                     EEGSAGGSFFQKGNASWYGREFQGRLTASGERFNMNDMTAAHKTLPFGTVLMVKSLDT
                     GKSVKVRINDRGPYKQGRIIDLSHSAAKKLDMLKTGEAMVGITIVKKGDGRRFSGRGD
                     RDDAIEPVAGDELDEGRDELPREGRGRSRDRGDRYGDSDSGSGGTALQAGAFYSRRNA
                     DGLKRKLEGMFDNPVEVVRDGDLYKVRVRGIRSMNDAKRYKRMLEKDSISSFIIQGRD
                     "
     gene            2223..3263
                     /locus_tag="PKJ16_05445"
     CDS             2223..3263
                     /locus_tag="PKJ16_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HNV46465.1"
                     /translation="MSIVSCKPTITRKELEGVLDCLINEDLLTGTTVKLFEGQASRHT
                     GIKYALAVNSLTAAYHLAFKALEIAPGDEIIIPSYFDIAPLSAADLIGATPVLVDIEQ
                     GALAPSTSQIKEKITEKTKAIVFGHTFGFLTPLEELAELGVPIVEDISHAIGGTLEND
                     APVGGGGAIAVASFAASMVITTGNGGMALTRNSRLYARMKELRGNGGGEHLHFDYCMT
                     DFQGAMGISQLARLEGFIYRRREIARSYYDALKLTPHKAPYHYSEQFVYQSFPIIFDA
                     PADKVEKYWKKNSIEVFHPVETPLHRYLGAAGHDYPHSDRASRKVYGLPIFPTLTRRE
                     IDKITTLLARFI"
     gene            complement(3281..3709)
                     /locus_tag="PKJ16_05450"
     CDS             complement(3281..3709)
                     /locus_tag="PKJ16_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HNV46466.1"
                     /translation="MKTIAAYIRRAEPGEPTLAAPEEQEREIRQYAITRYGDGKYKME
                     LFRELTPFNRVDGRPALHRMLEWLGAHSVAAVLVHRASCLFRTMEDGVVVGDFLRSHG
                     VELVSVVEGEIDLATVEKRLFGNVERLIEQHYAKREKKKR"
     gene            3784..5100
                     /locus_tag="PKJ16_05455"
     CDS             3784..5100
                     /locus_tag="PKJ16_05455"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46467.1"
                     /translation="MKYIPINTLCHLFVIDETSSFVIGSPPEWIKRATGVPFPRHILL
                     GDSLLVNGCNFAEIEFPLYKNFFFNKLRKTEIVCPEESTPRLAGILAEALLGPDYRLD
                     SPGSVKAAYFGDILAVRKNFDPSGEKLTLDDYATFHTYENDRFAIGDCAIRRVDRDAF
                     HVAWPGGEQSVSLEYRETSLDKRPASPRAIPGALSIFCFDSGDGFTPAKECSSFILNA
                     GGRYILFDPSLSSFDRLVERGCDFRDVAAVFISHVHADHDQGLYRFLMYNKNIIIMTG
                     ETVCESLAHKIGLMVGDANFATQCRITPIPLKQRTGVAELGIDVYCDLGFHSIPSAMA
                     KIYFHDAGLRDIVLAYSGDTLYDPVRIRSDSFNAVYREDLLRFYDDATHIIHEGGAGL
                     IHTDPEDLIPFLAPGQEIYWMHTGRTNDDGFSRGHILTRDENIVIR"
     gene            complement(5123..5635)
                     /locus_tag="PKJ16_05460"
     CDS             complement(5123..5635)
                     /locus_tag="PKJ16_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HNV46468.1"
                     /translation="MPNFTAIEPSDLADNVFRLIGSDWMLITAGDRESFNTMTASWGG
                     LGVLWHRPVSYIVVRPVRHTYRFLERGDAYTLSFFDARYKSVLQLCGSKSGRDIDKVK
                     EAGITPRTTELGSVYFEEARLVLECRKIYFQDIDPRNFVDQSIGENYPDRDYHRMYVG
                     QIVTCLKRSE"
     gene            complement(5693..6454)
                     /locus_tag="PKJ16_05465"
     CDS             complement(5693..6454)
                     /locus_tag="PKJ16_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46469.1"
                     /translation="MKLNRTALAVLLLAALALGCGTAGIATSSRESYRGDRLERIEGR
                     VALLPVTEADARTIGSLDAPSRAISAPYSDPIIDRGESVRFNAGTGPDRVNHVASDIL
                     LEKLRAMARGIEVIPPRRLESVVGRSAARAAFLELRRTYRHLGADPGLLRRLAGVTGC
                     RYLLVPQLVVISNVNDSHRSFVWSFGKRTSSYSVILLGDLWDLSAGELAWSGRGVAST
                     RVGTYEAPAFFDDLAALAAEEFVRALPATGPRATK"
     gene            6593..6961
                     /gene="queD"
                     /locus_tag="PKJ16_05470"
     CDS             6593..6961
                     /gene="queD"
                     /locus_tag="PKJ16_05470"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HNV46470.1"
                     /translation="MYILTIEETFASAHQLRGYRGKCENLHGHNWRVVMSVQGETLNA
                     IGLLVDFHDLKAALREVLAELDHRNLNEVPHFAEHNPSSENIARYIYERMAQRLAGGA
                     AAMESITVWESGTARCTYRP"
     gene            complement(6965..7894)
                     /locus_tag="PKJ16_05475"
     CDS             complement(6965..7894)
                     /locus_tag="PKJ16_05475"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HNV46471.1"
                     /translation="MNIYTDIPDCGAFANPVLTIGSFDGVHLGHHRILSVLLNTARER
                     SGDAVVLTFRDHPRAVLTPHTPPKILTTADEKIEAIGEHGVSHIIMLPFTRAMAEMSA
                     EEFLGEMVLKKLGVTGLVVGYDHAFGKNRAGTFDFLRSVSHERGFGVVRVEPRNFYSR
                     PVSSTWIRTELEDGNVALAAMLLGRRYRFGGTVVRGAGRGRTIGFPTANVRPDDPAKV
                     IPRDGVYAVTVSLPGGLRRQGMLNIGTNPTFAETERTIEVNIFDFEADIYGAPIGVFF
                     HERIRDEVRFADARELAAQIERDRRAALELLNE"
     gene            8004..8867
                     /gene="mtnP"
                     /locus_tag="PKJ16_05480"
     CDS             8004..8867
                     /gene="mtnP"
                     /locus_tag="PKJ16_05480"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HNV46472.1"
                     /translation="MEQATIGIIGGSGLYDIEGVKIVDEVSMDTAWGAPSDNVIIAEI
                     EGVRAAFLPRHGRGHVHLPMEINNRANIAALKMMGVRQVIAFSAVGSLTEMVHPLDFV
                     VPDQMIDRTRVRAATFFGNGVAAHIAFAHPFCDRLRHLVSARGALKGVALHDGGTLLC
                     MEGPAFSTRAESELYRAWGAQVINMSSSAEAKLAREAEMCYAVVCMATDYDCWKEDEE
                     HVTVDMLIANLNKNGENAKSMVKTLVPAMAAAKRDCGCGATVGSAIITAPEKRNPEQV
                     HKLRTILPEYF"
     gene            8915..9382
                     /locus_tag="PKJ16_05485"
     CDS             8915..9382
                     /locus_tag="PKJ16_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46473.1"
                     /translation="MSSTRNYIAHAARGILCCAVLLAGARDGWCLTPVGSYRDCLAAQ
                     ENIRPPEEVAGEHAEPESPLRRFEISFLISLPFVFAAHFLVLWSADVLIQGNTNVNVW
                     KNHGAFLAADTLLITTIIAYREASIVRDANREREERSGRATSRTFYLSWSHQW"
     gene            complement(9389..10270)
                     /locus_tag="PKJ16_05490"
     CDS             complement(9389..10270)
                     /locus_tag="PKJ16_05490"
                     /inference="COORDINATES: protein motif:HMM:NF017121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF726 domain-containing protein"
                     /protein_id="HNV46474.1"
                     /translation="MKIPANRIIGVLMALALLAVCALPACAPSRTSGSLPRSWSGAPE
                     DPGVTIRLVRDCVDCRAPVVVTVNGFLSEGDSLDDWRAALAERYGKCAWVHVDWEAAS
                     IASATAYLLTAGLAIAAIARTKKVSEFTADDWRRIAALVDANSWRRALRNAQTAGRAL
                     CRYLMAHRRDAPGDRYVLMGNSLGAGVVFVSLACLADAGIDDAVDSAHLLGGAVTNDT
                     EAWRHALRAVTGAVWNYYSRNDEVLAYLYPVATLFANAPAGRSPITGVRGVENIDVSA
                     RVSGHLNYKNNAAAFLR"
     gene            complement(10344..10979)
                     /locus_tag="PKJ16_05495"
     CDS             complement(10344..10979)
                     /locus_tag="PKJ16_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNV46475.1"
                     /translation="MSGDWFIGLAEGIIIGFSIAAPVGPIAILCVNRTIARGRLSGFV
                     SGLGAATADAVYGGIAAFGVSLILNFLMEHQRVIGIIGGIFLLVLGVRIFFTKLAQQR
                     IDDTSRTQVANFISTFILTLTNPMTLVAFAAVFAGLGITATGDALAGCFLLAGVFIGS
                     ASWWFLLTLGVSVLRPRIMLLAESFVNRVTGVVIFLFGIAVLFGVIERLRP"
     gene            complement(10976..11413)
                     /locus_tag="PKJ16_05500"
     CDS             complement(10976..11413)
                     /locus_tag="PKJ16_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46476.1"
                     /translation="MKKIACALALLAALATDARATEDLYQTVGAAGGSNLYVTGIAIG
                     VIADAYERKIYDADKATDLAVSIVGQAKVLRGFYRTLIEKNAMPQHDVSFMREMVLVY
                     GLLIAEGEALVKYIKTGNGEHASEYHKNRTDAWARIEKLLNMQ"
     gene            11495..13306
                     /gene="pepF"
                     /locus_tag="PKJ16_05505"
     CDS             11495..13306
                     /gene="pepF"
                     /locus_tag="PKJ16_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HNV46477.1"
                     /translation="MAPDGTLMSRAEQRPGDTWDLSPLFTGADEWEALFARVERDSAR
                     YAEFRGRLGESPATLAAALEFDLALSRDAERIYTFAHLKSDEDKTDQTALALYERASA
                     LFTRLSELSSFMTPELQAIPDNAMRALMDDPLLAGYRFFLEKIIRFKPHTLSQEIEEV
                     LAMSGEMAHAPRQFFGQLDNADLRFGVITDESGAQVELTHGNFSSFLMKRDRELRRRA
                     FFQYYKTYEEHKHAIAAALAASCKKDRFYARVRRFGSCREASLFSDNVPPSVYDNLLG
                     AVKENLGPLFTYLRFRRERLGIDELHFYDTYVPLVDGVDFRMPYEEAVDTCAAALAPL
                     GTEYVDTLRAGLLERRWVDRYENRGKRSGAYSSGCYDSPPYILMNYREDNINSLYTLI
                     HEAGHSMHTHSSARHQPYVYHEYTIFVAEVASTFNEVLLSRHLLERYRDDPAMRAYIL
                     NREIDDIRGTLFRQTMFAEFEKRTHELVERDQPLTLDAFTGIYRELLSAYFGDTLVVD
                     DALALECLRIPHFYSAFYVYKYATGISAAIALAETVLAEGARARDRYLDFLKTGGSMF
                     PLDELRGAGVDMETPGPVRDAIAHFGRLVGELRAALG"
     gene            complement(13331..14518)
                     /locus_tag="PKJ16_05510"
     CDS             complement(13331..14518)
                     /locus_tag="PKJ16_05510"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HNV46478.1"
                     /translation="MNGTGASLVVTLKRGREKSVRARHHWIFSGAVARVEGEASPGAI
                     AAVRDSAGAVLGYGCYGGRASIAVRMLTFGDRPFTTDVLRGLIRAAIARRRDTPLLAG
                     TDAVRLVFSEGDALPGLIVDSYNGHLVLQSLTAGIDRLRNDIAGILVDELAPASLYER
                     SDHAGRALEGLSEESGQLVGSTPDAVEIHEGGVVFVVDITRGQKTGFFLDQRDNRALV
                     RSVAAGRRALDLFCYQGGFSLAALAGGARSCVAVDASEAACAAARRNIAHNAFDAPAE
                     LVCADVFEFIRARAIDADLVIIDPPAFAKSREAVARAARGYKDINLNVLRACPPGSLV
                     LTCSCSRFVDAALFQKIVFGAAADANRAVSILRRTHQPADHPVNIYHPETEYLTALLL
                     SVE"
     gene            complement(14515..15183)
                     /locus_tag="PKJ16_05515"
     CDS             complement(14515..15183)
                     /locus_tag="PKJ16_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HNV46479.1"
                     /translation="MPIETPHDAMYTAYCSGPLFCPEEAAGMSAIAARLEEAGFATFL
                     PHRDGIEAYVMRLANSPLNVNLLRVRDAIDRAIFALDVYQLAERCDGMAINLNGRVPD
                     EGAVVEASLAFALGRPVVAYKNDARAPFRGRDNSMLAGLYGTRVDDIGAIPHALRNEI
                     ERKRRTDASQAAPCTDELRRAIDLGRTIWRFLERLPSGRRRPEAAVKEIARLCREDGR
                     RYAR"
     gene            15315..16445
                     /locus_tag="PKJ16_05520"
     CDS             15315..16445
                     /locus_tag="PKJ16_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HNV46480.1"
                     /translation="MKNILIYSDTLASFAYGAYHPYKPERARQFKELAARYGLLYEAN
                     QEIVEPKPLDEELLYLFHDRDYIDLLKASERGAPTVEMLEAGLGSPDNPIIPGMYEFA
                     LLAAGGTLLGAQLLDEDAARFVFNPSGGFHHAGRAHAEGFCYINDIGVTIEHLIRRGR
                     RVAYIDIDVHHGNGVQDAFYDSDRVLTISIHESGVSLYPWSGFENEIGEGAGRGYNVN
                     VPLLPETDDHAYWYAFESVVPPLVTHFAPDIVFAEIGGDLHRDDPLANLNITTEVWCR
                     AVGAINALAPRIMALGGGGYNPHKTAALWALAWAIFCGLTPSDSYAGIVGGMMYGPEA
                     QAGSLHDEPSAAESCALKPARHAHDVVRHIRETVFPLHGIAA"
     gene            16504..17037
                     /locus_tag="PKJ16_05525"
     CDS             16504..17037
                     /locus_tag="PKJ16_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNV46481.1"
                     /translation="MPKKVHPTATVRGDGIPLIIEKKSKLYFQNVSDSVVFIFKTLLG
                     IDATPKKLFRRRLTSLPNDIIIKLDFAADLTGHFAFFLSLKTAFEICNRLVPGIGYQY
                     FDKDHLDVIGEMGNMISGNAIGRLQNIDSAIDLSIPSMTAFHTILESESRYWSFSGNF
                     DVDIGQVGILLAIRKDK"
     gene            17351..18751
                     /locus_tag="PKJ16_05530"
     CDS             17351..18751
                     /locus_tag="PKJ16_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HNV46482.1"
                     /translation="MINRGTINSIFERLRAELGDASVTRDGDILAAYSRDETSDLAAA
                     PDILVRARSATDVAAVMRACNERRVPVVPRGAGTGVTGGAVATAGGVVLSLERMNRII
                     EIDAKNMIAVVEPGAITGEIQRSAAAAGLMYPPDPASLDSCSIGGNIAEGAGGPRAVK
                     YGTTKDYVTGLEFVTPEGHIITAGGKYVKNATGYNLVGAIIGSEGTLVVVTKIFLKLL
                     PAPRASLDLLVPFDSLDSAVDTVTAILHSGVLPAAIEFMEADAIDLVATFLHTAMPFP
                     DARAHLLIQLDGASTAEVEHATETIARVLPVPAERVIAAQDPAQRERIWKARRSIREA
                     IHAESPVFLAEDSVVPRNEIPAFLADVKAELQRRALRSVMFGHAGDGNVHIDVLKGAM
                     DDAQWGALVPELKGMIYRAAIARGGTITGEHGTGFTRKRYLGLAYSAEAIELMRRIKT
                     AFDPNGILNPGKIIDG"
     gene            complement(18786..19187)
                     /locus_tag="PKJ16_05535"
     CDS             complement(18786..19187)
                     /locus_tag="PKJ16_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNV46483.1"
                     /translation="MRIVLDTNVLVSAILNPRGKPADILNLVLDGRVVVCYDDRIFIE
                     YRGVLLRERFRFHPDDVESLLLFIAHAGERIVPVPRALVLPDPDDLMFYEVLAASSAK
                     FLVTGNVKHFLAANDRRVVSPQKFLEKYFLG"
     gene            complement(19184..19465)
                     /locus_tag="PKJ16_05540"
     CDS             complement(19184..19465)
                     /locus_tag="PKJ16_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNV46484.1"
                     /translation="MKFITVRDLRTKPAAIWRDLKKERELVITNNGKPIGLITPILES
                     GLEELLRAVRKARAEEALRSMRRTADDGDTISLSDEEISREIHRYRSGT"
BASE COUNT         3676 a         6575 c         6186 g         3423 t
ORIGIN      
        1 ccttcgccat ggcctaatcc aggagcccct tgaccagcat gcccagcacg gtgtcgacgc
       61 catagagaaa cagcgaaacg atcacgacgg cgacgatgac gatcatcgtg aagctggtca
      121 cctcatcgcg atcgggccag gtgacccgct tcagctcgtc ccgcgattcg cggacaaatt
      181 gtatcacttt cttgaacaca acgcctccgt cccgtccgat cgcggccgtt tatgccttgg
      241 tttccttgtg cagggtgtgc ttgttgcaga acttacagta cttcatgacc tcgagcttgc
      301 cggtctgggt cttcttgttc ttggtcaggc tatagttctt gcgcttgcag tccttgcagg
      361 acaatagtat gatgtctctc attcgccaaa cccgcttgtg gaattcaatg ttcgattaga
      421 gtaatgagcg cggtcggcca cgcccccgcg caggaggaca aacgaaacgc acagctcgcc
      481 ccgaaactct tccgtgaaat gtgcattaca tttttatgtg ccaaaaccgt caactatttt
      541 gcgcaaaaaa cacaaataaa atcacgcatg atcccgccct gcggccgcca tcctcatcaa
      601 agcgatgcct gtcccggccc aattgcacat cgagcccagg atgtccagtt atggcatgat
      661 caaaaatcgg aatcgcacga tgaaggccga tggcctccgg cgcatatgca tcgacgccgg
      721 ccagtggaag cgatgccggg gaggcccggc cgcgcatgca atgcacccca tcgatgagcc
      781 cacgaccaga atcgaactgg tgaccttttg cttaccatgc aaatgctcta ccgactgagc
      841 tacgtgggcg cgaacgcgga gtcaccttgg ggcagggcgc ccggttttgt caatgttttt
      901 acggaaaatg gaccgaaaag acacctcggg ccccgcacgc cttaccgtat ggcgacactc
      961 ggggcccgtc tggctcaagg cgtgtttatc cgagaaagac gctcaatccc gaccctgaat
     1021 tataaaggaa gagatgctgt ccttttcgag catgcgtttg tatcgcttgg cgtcattcat
     1081 gctgcgaatg ccgcggacgc gcaccttgta gaggtcgccg tcgcgcacca cctcgacggg
     1141 attgtcgaac atgccctcaa gcttgcgctt gaggccgtcg gcattgcgac gggaatagaa
     1201 ggcgcccgcc tgcagcgccg tgccgccgga accgctatcg gaatcaccat accggtcgcc
     1261 gcggtcgcgg gagcggcccc taccctcccg gggcagctcg tcgcgcccct cgtcgagttc
     1321 gtcgccggcc accggctcaa tggcgtcgtc gcgatcgccc cgtccgctga agcgacgacc
     1381 gtcgcctttc ttgacgatgg tgatgcccac catggcctcg ccggttttga gcatgtcgag
     1441 ctttttggcc gccgaatgcg acaggtcgat gatgcgcccc tgcttatacg ggcctcgatc
     1501 gttgatgcgc accttgacgg acttgcccgt gtcgaggctt ttaaccatga ggacggtgcc
     1561 gaacggcagc gtcttgtgcg ccgcggtcat atcattcatg ttgaagcgct cgcccgaggc
     1621 cgtgaggcgc ccctggaatt cgcgcccgta ccacgaggcg ttccccttct ggaaaaacga
     1681 cccgccggca gagccttcct cgtcgtcgaa ggagcgctcc tcgtcgcgat cgatcgagcg
     1741 cgagcgggac gcattgcgca ccgggcgcac cccctcgttc gcctccgcat cgcgctcggc
     1801 ccattgctgc ttcttcccgg ccgcgtttcg gtcgagcgcg tcgtaatcgc gatcgttctc
     1861 atcgtacgaa aaatcgtcgc tgtcccccgc tgcgaccgcc cggtcgccgg ccccggtgcg
     1921 cgcgtgccgc ggggcgcacg agctcgcgct caacatcgcc gccacgaaga cgacgaccag
     1981 aatcgtcccg aatatccgtt ttgtcatgaa atacacctcc cctcgctccg gccgtgttgc
     2041 acggcatgta tcgtgtttcg ggatcaatcc cgttgtgctt gtttccgaac gcatgatggt
     2101 acctcactcc atgtatcggc ggattaaaaa aatgccttta cagggaaagc gaaaaatatt
     2161 atgtcggatt tcgcgtcccg ccgaaaccgg cggcgcgcac cacgcgcggg gtaccgacag
     2221 ccatgagcat tgtttcgtgc aagccaacca tcacccggaa ggagctcgag ggggtcctgg
     2281 actgcctcat caacgaggac ctgctcaccg ggacgaccgt gaagctcttc gaggggcagg
     2341 cctcgcggca taccgggatc aagtacgcgc tcgcagtgaa ttcgcttacc gcggcctatc
     2401 atctcgcctt caaggcgctc gagatcgcgc cgggcgacga gatcatcatc ccctcgtatt
     2461 tcgacatcgc ccccttgagc gcggccgacc tgatcggcgc cacgcccgtg ctggttgaca
     2521 tcgaacaggg ggcgctggcc ccatcaacct cgcagataaa ggaaaagatc accgagaaaa
     2581 cgaaggctat cgtctttggg cacaccttcg gtttcctgac gccgttggag gagctcgccg
     2641 agctcggcgt cccgatcgtc gaggacatct cgcacgcgat cggcgggacc ctcgagaacg
     2701 acgcgccggt cggcgggggc ggcgcgatcg cggtggcctc gttcgcggcc tccatggtca
     2761 tcacgacggg aaacggcggc atggcgctca cgcgaaactc gcggctctac gcgcgcatga
     2821 aggagctgcg ggggaacggc gggggcgagc acctgcattt cgattactgc atgacggact
     2881 tccagggggc gatgggcatc tcgcagctcg cgcgcctcga ggggttcatc taccgccggc
     2941 gggagatcgc gcgctcctac tacgacgcgc tcaagctcac gccgcacaag gcgccctacc
     3001 actacagcga acagttcgtg taccaatcat tcccgatcat cttcgacgcg ccggccgaca
     3061 aggtggaaaa atactggaag aagaacagca tcgaggtgtt tcatcccgtg gagacgcccc
     3121 tgcaccgcta tctgggcgcg gcgggacacg actatcccca cagcgaccgc gcatcccgca
     3181 aggtctacgg cctgccgatc tttccgacgc tcacccgtcg cgagatcgac aagatcacca
     3241 cattgctcgc gcgcttcatc tgacccgccg cacagcccaa tcagcgcttc ttcttctccc
     3301 gtttcgcata gtgctgctcg atgagccgct ccacattgcc gaagaggcgc ttctcgacgg
     3361 ttgcaaggtc gatctccccc tcgacgacgc tcacgagctc gaccccgtgc gaacggagaa
     3421 aatcaccgac gaccaccccg tcctccatgg tgcgaaagag gcaggaggcg cgatgcacca
     3481 ggaccgcggc gacgctatgc gcgcccaacc actccagcat gcggtggagg gccgggcggc
     3541 cgtccacccg gttgaagggc gtgagctccc gaaaaagctc cattttgtat ttgccatccc
     3601 cgtagcgcgt gatagcgtac tgtcgtattt cgcgctcctg ctcctcgggc gctgcgaggg
     3661 tcggctcgcc cggctcggcg cgccggatat aggcggctat ggtcttcatg ggcatgttcc
     3721 gctcaagttc atcggtggtc gcgtccgata gtagagagca aggggaaata attcaacata
     3781 aatatgaaat acattccgat caacaccctg tgccacctgt tcgtgatcga cgagacgagc
     3841 tcgttcgtga tcggctcacc gccggagtgg atcaagcgag cgaccggcgt gccgttcccc
     3901 cggcacattc tgctcgggga ctctctgttg gtcaacggtt gcaacttcgc cgagattgag
     3961 tttcccctgt acaagaactt cttcttcaac aaactgcgca aaaccgagat cgtgtgcccc
     4021 gaggagagca cgccgcgcct tgcgggcatt ttggccgagg ccctcttggg accggactac
     4081 cgcctcgatt cacccggcag cgtcaaggcc gcctatttcg gcgacatcct cgcggtgcgc
     4141 aagaacttcg atccctccgg ggaaaagctc acgctggacg attacgcgac cttccacacc
     4201 tacgaaaacg accggttcgc cataggcgac tgcgcgattc gcagggtgga ccgggacgca
     4261 ttccatgtcg cctggcccgg gggcgaacag tcggtctcgc tcgagtaccg cgaaaccagc
     4321 ctcgacaagc gcccggcctc accgcgcgcg attccgggcg cgctttcgat cttctgcttc
     4381 gattcgggcg acggcttcac ccccgcgaag gagtgctcgt cgtttatcct caacgccggg
     4441 ggacgctaca tcctgttcga tccgagcctg agctcgttcg accgcctggt ggagcggggg
     4501 tgcgatttcc gcgacgtcgc cgcagtgttc atttcccatg ttcacgccga ccacgaccag
     4561 ggcctctacc gctttttgat gtacaacaag aacatcatca tcatgaccgg cgagaccgtg
     4621 tgcgagtcgc tggcgcacaa gatcgggctc atggtgggcg acgccaattt cgcgacgcag
     4681 tgccgcatca cgcccatccc gctcaaacag cgcaccggcg tcgcggagct cggcatcgac
     4741 gtctactgcg acctcgggtt tcactcgatt ccatcggcga tggcgaagat atattttcat
     4801 gatgcgggtc tgcgcgacat cgtgctcgcc tactccggcg acacgctcta cgacccggtg
     4861 cggatccgct ccgattcgtt caacgcggtc tatcgcgagg acctgttgcg cttctacgac
     4921 gacgcgacgc acatcatcca cgagggcggc gccgggctca tccacaccga cccggaggac
     4981 ctgatcccct tcctcgcgcc gggccaggag atctactgga tgcacaccgg ccgcaccaac
     5041 gacgacggct tctcgcgcgg ccacatcctg acgcgcgacg agaacatcgt catccgctaa
     5101 cgcgcggccg gtcccctagc gcctattcgc tccgcttcag gcacgtgacg atctgcccca
     5161 cgtacatgcg atggtagtcg cggtcgggat agttctcgcc gattgattgg tccacgaaat
     5221 tccgtgggtc gatgtcctga aagtatatct tccggcactc gagcacgagg cgcgcctcct
     5281 cgaaatacac cgaacccagc tccgtcgtgc gcggggtgat ccccgcctcc ttcaccttgt
     5341 cgatgtcgcg ccccgatttc gatccgcaga gctggaggac gctcttgtat cgcgcgtcga
     5401 aaaacgagag cgtataggcg tcgccgcgct ccagaaaccg gtaggtgtgc cgcaccgggc
     5461 gcaccacgat atagctcacg ggccggtgcc acaggacccc aagcccgccc caggaggcgg
     5521 tcatcgtatt gaacgactcc cggtccccgg cggtgatgag catccagtcg ctcccgatga
     5581 gccgaaacac gttgtcggcg agatcgctgg gctcgatggc ggtaaagttc ggcatgtcgt
     5641 tcctcctgca gagccggacc ggcccgttgg gccgtcccgg agacggtatg cgttattttg
     5701 ttgcgcgcgg accggtcgcg ggaagcgcgc gaacgaactc ctcggcggcg agcgccgcaa
     5761 gatcgtcgaa gaatgccggc gcctcgtagg tccccacgcg cgtggaggcc acgccgcgcc
     5821 cgctccaggc gagctcgcct gcggaaaggt cccacaggtc gcccagcagg atcaccgaat
     5881 agctgctggt gcgcttgccg aagctccaca cgaaggagcg gtggctgtcg ttcacgttcg
     5941 agatgaccac cagctgcggc accagcaggt aacggcagcc ggtcacgccc gccaggcgcc
     6001 ggagcagtcc gggatcggcc cccaggtgcc ggtaggtgcg ccgcagctcg aggaaggcgg
     6061 cacgcgcggc gctgcgcccc accacgctct cgaggcggcg cggggggatc acctcgatcc
     6121 cgcgggccat tgcgcgcagc ttctcgagca ggatgtcgct ggcgacgtgg ttcacgcggt
     6181 cggggcccgt cccggcattg aagcggacgc tctcgccgcg gtcgatgatg gggtccgaat
     6241 acggtgcgct gatggcccgc gacggcgcgt cgagcgagcc gatcgtccgc gcatcggcct
     6301 ccgtgaccgg gaggagcgcg acgcgcccct cgatgcgctc gaggcggtcc ccgcgatacg
     6361 actcccggga cgaggtcgcg atgccggcgg tgccgcagcc gagcgccagc gccgcgagca
     6421 ggagtacggc gagagccgtt cggttcagtt tcatggcgag caattccatc cgtgcgcgtc
     6481 gttgctatcg gcacagcata gccggtgaaa ccgttttatc aagaaaatta ttccgcatgc
     6541 ggccggaaat tctgtttgcg caaacgccgc cgccgatggc gtgttggcgc ccatgtatat
     6601 ccttacgata gaagaaacct tcgcctccgc gcaccagctt cgcggctacc gcgggaaatg
     6661 cgaaaacctg cacgggcaca actggcgcgt ggtgatgagc gtccagggcg agacgctcaa
     6721 cgcgatcggc ctcctggtgg acttccatga cctgaaggcg gcgcttcgcg aggtgcttgc
     6781 cgagctggac caccggaacc tgaacgaggt gccgcacttc gccgaacaca atcccagctc
     6841 ggagaacatc gcgcgctaca tttacgagcg aatggcgcag cggctcgccg gcggcgccgc
     6901 agcgatggaa tcgattacgg tgtgggaatc gggcacggcg cggtgcacct accggccctg
     6961 aggatcattc gttgagcagc tcgagcgccg cgcggcggtc gcgttcgatc tgcgccgcga
     7021 gctcgcgcgc gtccgcaaag cggacctcat cgcgaatgcg ttcgtggaag aacacgccga
     7081 tgggcgcgcc gtagatatcg gcctcgaaat cgaagatatt cacctcgatg gtgcgctccg
     7141 tctcggcgaa ggtcggattg gtcccgatgt tgagcatgcc ctgccggcgg aggccgcccg
     7201 gcaacgacac ggtcaccgcg tacacgccgt cgcggggaat caccttggcg ggatcgtccg
     7261 ggcggacgtt cgccgtggga aagccgatgg tgcgcccccg ccccgccccg cgcaccacgg
     7321 tcccgccgaa gcggtaccga cggcccagca gcatcgccgc cagcgcgacg tttccgtcct
     7381 cgagctccgt gcgtatccag gtactggaca ccgggcgcga gtagaagttc cgcggctcca
     7441 cccggaccac gccgaacccg cgttcgtgcg aaacgctgcg caggaagtcg aaggtccccg
     7501 cgcggttttt cccgaaggcg tgatcgtacc cgaccacgag gccggtgacc cccagcttct
     7561 tgagcaccat ctcgcccagg aactcctccg cgctcatctc ggccatcgcg cgcgtgaagg
     7621 ggagcatgat gatatgcgaa accccgtgct cgccgatggc ctcgatcttt tcgtccgcgg
     7681 tggtgaggat cttcggcggc gtgtggggcg tgagcaccgc gcggggatgg tcgcggaagg
     7741 tgagcacgac cgcgtcgccg gagcgctcgc gcgcggtgtt gagcagcacc gacaggatgc
     7801 ggtgatggcc gaggtggacg ccgtcgaagc tgccgatggt gaggaccgga ttggcgaagg
     7861 cgccgcaatc ggggatgtcg gtgtagatgt tcatgcgcgg cggctattgt cgggccgcgc
     7921 gcgaaacaat caattagaat tttaattgat ttatccgcgc ccggcggccc actatgtcca
     7981 tccagaaaac caggagagca tcaatggaac aggcgaccat cggcatcatc ggcggctccg
     8041 ggctgtacga catcgagggc gtgaagatcg tggacgaggt gtcgatggac acggcgtggg
     8101 gcgcgccctc cgacaacgtc atcatcgcgg agatcgaggg cgtgcgcgcg gcgtttctgc
     8161 cccggcacgg gcgcgggcac gtgcacctgc cgatggaaat caacaaccgg gcgaacatcg
     8221 ccgcgctcaa gatgatgggc gtgcgccagg tgatcgcctt ctccgccgtg gggagcctca
     8281 cggagatggt gcacccgctc gacttcgtgg tgcccgacca gatgatcgac cgaacgcgcg
     8341 tgcgcgccgc caccttcttc ggcaacgggg tcgccgcgca catcgccttc gcgcacccct
     8401 tctgcgaccg cctgcgccac ctggtgtccg cccggggcgc gctgaagggc gtggccttgc
     8461 acgacggcgg gacgctcctg tgcatggagg gcccggcctt ttcgacccgc gccgagagcg
     8521 agctctatcg cgcctggggc gcgcaggtca tcaacatgag ctcgtcggcc gaggccaagc
     8581 tcgcgcgcga ggcggagatg tgctatgcgg tggtgtgcat ggcgaccgac tacgactgct
     8641 ggaaagagga cgaggagcac gtgacggtgg acatgctcat cgccaacctg aacaagaacg
     8701 gcgaaaacgc gaagtcgatg gtgaagacgc tggtgcccgc gatggccgcc gcgaaacgcg
     8761 attgcggctg cggcgcgacc gtcggctcgg cgatcatcac ggcgccggag aagcgcaacc
     8821 ccgaacaggt gcacaagctc cgaacaatac tgccggaata tttttaacgg cccgcggcct
     8881 cgcgcggaaa cggaagggcc gtcggcgtac catcatgtca tcaacgagaa attacatcgc
     8941 ccacgcggcc cgcggcatcc tctgctgcgc cgtcctgctc gccggcgcgc gcgacggctg
     9001 gtgcctcacg cccgtcggct cctaccgcga ttgcctcgcc gcgcaggaga acatccggcc
     9061 ccccgaggag gtcgccggcg agcacgccga gccggaatcg ccgctgcgtc gtttcgagat
     9121 cagctttctc atctccctgc cgttcgtgtt cgccgcgcac ttcttggtcc tctggagcgc
     9181 ggatgtgctc attcagggca acacgaacgt gaacgtgtgg aaaaaccacg gggccttcct
     9241 ggcggccgac acgctgctga tcaccaccat catcgcctac cgcgaggcga gcatcgttcg
     9301 cgacgcgaac cgcgagcgcg aggagcgcag cggccgcgcg acgtcgcgaa ccttctacct
     9361 ctcctggtcg caccaatggt aacgccggct agcgcaaaaa cgccgcggcg ttgtttttgt
     9421 agttcaggtg ccccgacacg cgcgcgctca catcgatgtt ttcgacgccg cggacgcccg
     9481 tgatgggact tcgccccgcc ggcgcgttcg cgaacagggt cgcgacggga tacagatagg
     9541 cgagcacttc gtcgttgcgc gagtaataat tccacacggc gcccgtgacg gcgcgcagtg
     9601 catggcgcca cgcctcggta tcgttcgtca ccgcaccgcc caacagatgg gccgaatcga
     9661 ccgcgtcgtc gatcccggcg tcggccaggc aggcgagcga gacgaacacc acgcccgcac
     9721 cgagcgaatt ccccatgagg acataccggt ctcccggcgc atccctccgg tgcgccatca
     9781 ggtaccggca gagggcgcgt ccggccgtct gtgcgtttcg aagcgcgcgg cgccacgagt
     9841 tcgcgtcgac gagcgcggcg atgcgccgcc agtcgtcggc ggtgaactcg ctcaccttct
     9901 tggtgcgcgc gatcgcggcg atcgccaggc cggccgtcag cagataggcc gtggccgagg
     9961 cgatgctcgc cgcctcccag tccacatgca cccaggcgca tttcccatag cgctccgcga
    10021 gcgccgcgcg ccagtcgtcg agcgaatccc cctccgacag aaagccgttc acggtgacca
    10081 cgacgggcgc gcggcagtcg acgcagtccc gcaccagtcg gattgtaaca ccgggatcct
    10141 ccggcgcgcc cgaccaggac cgcgggagcg atccggacgt ccgcgagggc gcacaggcgg
    10201 gcagcgcgca aacggcgaga agcgcgagcg ccatgagcac tccgataatt cgattggcag
    10261 gaatcttcat gtgcatgagc gcagtgtacc ggcggacgga ggggcctgtc aagcgttatg
    10321 atccctccgg cgaatcggaa tggtcacggc cgcagccgct ctatcacccc gaacaggacg
    10381 gcgatgccga acaggaagat gacgacgccc gtcacccggt tcacgaagct ctccgccaga
    10441 agcatgatgc ggggccgaag gacgctcacg ccgagcgtga gcaggaacca ccacgaggcc
    10501 gaaccgatga acaccccggc cagcagaaaa cagccggcca gcgcgtcgcc cgtcgccgtg
    10561 atgcccaacc ccgcgaagac cgcggcgaaa gcgacgaggg tcatgggatt ggtgagggtg
    10621 aggataaaag tggagatgaa gttcgcgacc tgggtccggc tggtgtcgtc gatgcgctgc
    10681 tgcgcgagtt tcgtgaaaaa gatgcgcacg cccagcacca gcaggaatat cccgccgatg
    10741 atcccgatga cgcgctggtg ctccatgagg aaattgagga tgagcgacac cccgaaggcg
    10801 gcgatcccgc catagacggc atcggccgtg gcggcgccga gcccggagac gaagcccgag
    10861 agccgtccgc gcgcgatcgt ccggttgacg cacaggatgg cgatgggccc gaccggagcc
    10921 gcgatcgaga agccgataat gatcccctcg gccagcccga tgaaccagtc gccgctcatt
    10981 gcatgttgag gagcttctcg atccgggccc aggcgtcggt ccggttcttg tggtattcgc
    11041 tcgcgtgctc gccgttcccg gttttaatgt atttgaccag cgcctccccc tcggcgatga
    11101 gcagcccgta gaccagcacc atctcgcgca taaaggagac gtcgtgctgc ggcatcgcgt
    11161 ttttttcgat gagggtccgg tagaagccgc gaagcacctt cgcctggccg acgatactca
    11221 ccgcgaggtc ggtcgccttg tcggcgtcgt atattttgcg ctcgtaggcg tccgcgatca
    11281 cgccaatcgc gatgccggtc acatacaggt ttgatccgcc ggccgcgccg accgtctggt
    11341 agaggtcctc ggtggcccgc gcgtccgtcg ccagcgcggc gaggagggcg agcgcacagg
    11401 cgattttttt cattgcgcgc tccttgactt tttacgaaca ctatggcaga gtgcgattat
    11461 tgcaagaatt ttattgcgca cccaaggagg gaggatggca ccggacggaa cgttgatgtc
    11521 gcgcgccgag cagcgccccg gcgacacctg ggatctgtcg ccattgttca cgggcgcgga
    11581 cgaatgggag gcgctcttcg cgcgcgttga gcgcgacagc gcgcgctacg cggagttccg
    11641 gggccggctg ggggaatcgc ccgcgacgct cgcggcggcc ctcgagttcg atctcgcgct
    11701 ttcgcgcgac gccgagcgga tctacacctt tgcccacctg aagagcgacg aggacaagac
    11761 cgatcagacg gcgctcgcgc tctatgagcg cgcatcggcc ctgttcacgc gcctctccga
    11821 gctctcgagc ttcatgaccc ccgagctcca ggcgatcccc gacaacgcca tgcgcgcgct
    11881 catggacgat ccgctcctcg ccggctaccg cttcttcctc gagaagatca tccgcttcaa
    11941 gccgcacacg ctctcccagg agatcgagga ggtgctggcg atgtccggcg agatggcgca
    12001 tgcgccgagg cagttcttcg gacagctcga caacgccgac ctgcgcttcg gcgtcatcac
    12061 cgacgagtcg ggcgcccagg tggagcttac ccacggtaac ttcagctcct tcctgatgaa
    12121 gcgcgaccgc gaactccgcc gacgcgcctt ctttcaatac tacaaaacgt acgaggagca
    12181 caagcacgcc atcgccgccg cgctggccgc atcgtgcaaa aaggaccgct tctatgcgcg
    12241 cgtgcgccgc ttcggatcct gccgcgaggc ctcgctcttt tcggacaacg tcccgccgag
    12301 cgtctacgac aatctcctcg gcgcggtaaa ggaaaacctg gggccgctgt ttacctatct
    12361 gcgctttcga cgcgagcggc tggggatcga cgagctgcac ttctacgaca cctacgttcc
    12421 actcgtggac ggcgtggact tccgcatgcc ctacgaggag gcggtggaca cctgcgcggc
    12481 cgccctcgcg ccgttgggaa cggagtatgt cgatacgctg cgcgcggggc tcctcgagcg
    12541 gcgctgggtg gaccgctacg aaaaccgcgg caagcgcagc ggcgcctact cgtccggctg
    12601 ctacgactcg ccgccgtaca tcctcatgaa ctaccgcgag gacaacatca acagcctcta
    12661 cacgctgatc cacgaggccg gccactccat gcacacgcac tcttcggcgc ggcaccagcc
    12721 ctatgtgtat cacgagtaca ccatcttcgt cgcggaggtc gcctccacct tcaacgaggt
    12781 gctcctctcg cgccacctcc tggagcgcta ccgcgacgac ccggcgatgc gcgcctatat
    12841 cctcaaccgc gagatcgacg acatccgcgg cacgctgttc cgccagacga tgttcgccga
    12901 gttcgagaag cgcacgcacg agctcgtcga gcgcgatcaa ccgctcacgc tcgacgcgtt
    12961 caccgggatc taccgcgagc ttctttcggc ctatttcggc gacacgttgg tcgtcgacga
    13021 cgccctggcg ctcgagtgcc tgcgcatccc gcacttctac tcggccttct atgtgtacaa
    13081 gtacgcgacc ggcatctccg ccgcgatcgc gctggccgag acggtgctcg cggagggcgc
    13141 acgtgcgcgg gaccgctatc tcgactttct caaaaccggc ggaagcatgt tcccgctcga
    13201 cgagctgcgc ggtgcgggcg tggacatgga gacgccgggg ccggtgcgag acgccatcgc
    13261 ccacttcggg aggctggtgg gcgagctccg cgccgccctc ggctaggccc gtccgtcgcg
    13321 tgacgccccg tcactccacg gagagcagga gcgccgtcag gtattccgtt tcggggtgat
    13381 agatgttcac gggatggtcg gccggctgat gcgtacgccg gaggatggac accgcgcggt
    13441 tcgcgtccgc cgccgcgccg aagacgatct tctggaagag cgctgcgtcc acgaaacgcg
    13501 agcacgagca ggtgagcacg agcgagcccg gcgggcaggc gcgcagcaca ttgaggttga
    13561 tgtccttata gccgcgcgcg gcgcgcgcga ccgcctcgcg gctcttcgcg aaggccggcg
    13621 gatcgatgat gacgaggtcg gcgtcgatcg cgcgggcgcg gatgaactcg aagacgtcgg
    13681 cgcaaacgag ctcggccggc gcgtcgaagg cgttgtgcgc gatattccgg cgcgcggccg
    13741 cacacgccgc ctccgaggcg tccaccgcga cgcatgaacg cgcgcccccc gcgagcgccg
    13801 caagcgagaa tcccccctga tagcagaaga ggtcgagcgc gcgccgaccc gcggcgaccg
    13861 accgaaccaa cgcgcgattg tcgcgctgat cgaggaaaaa gccggtcttt tgcccgcgcg
    13921 tgatatcgac gacgaagacg acgccgccct cgtggatttc gaccgcgtcg ggcgtcgagc
    13981 cgacgagctg accgctctcc tccgacaacc cctccagcgc cctccccgca tgatcgctcc
    14041 gctcgtacag gctcgccggg gcgagctcgt cgacgagtat accagcgata tcatttcgaa
    14101 ggcggtcgat cccggccgtg agcgactgaa ggacgagatg gccgttgtac gaatcgacga
    14161 tgaggccggg gagcgcgtcg ccctcggaga agacgagccg gacggcgtcg gtccccgcca
    14221 ggagcggagt atcgcgccgg cgcgcgatgg cagcgcggat cagcccccgc aggacgtcgg
    14281 tcgtgaaggg acggtcgccg aaggtgagca tgcgcacggc gatgctcgca cggccgccgt
    14341 aacagccgta gcccaggacc gcgcctgcgg aatcgcgcac cgcggcgatc gcgccgggcg
    14401 aagcctcgcc ctccacccgc gcgacggcgc cggagaatat ccaatgatgg cgcgcgcgca
    14461 cggacttttc gcgcccgcgc ttgagcgtaa cgacgaggga tgcgccggtt ccgttcatcg
    14521 ggcgtaacgc ctcccgtcct cgcggcacag gcgcgcgatc tccttgaccg cggcctccgg
    14581 ccggcggcgc cccgacggca ggcgctcgag aaagcgccag atggtccgcc caagatcgat
    14641 ggcccgccgc agttcgtccg tgcatggcgc ggcctgcgat gcatccgttc gcctcttccg
    14701 ctcgatttca ttgcgcagcg cgtgcgggat cgcgccaatg tcatcgaccc gcgttccgta
    14761 gaggcccgcg agcatggaat tgtcccttcc ccgaaagggg gcgcgcgcat cgttcttgta
    14821 ggcgaccacg ggccttccca gcgcgaaggc gagcgacgcc tcgacaacgg cgccctcgtc
    14881 gggcacgcgc ccgttgaggt tgatcgccat gccgtcgcag cgctcggcga gctggtagac
    14941 gtcgagcgcg aagatggcac ggtcgatcgc gtcgcggacg cgcaggaggt tcacgttgag
    15001 cggcgagttc gcgaggcgca tcacgtacgc ctcgatcccg tcgcggtgcg gcaggaaggt
    15061 cgcgaagccc gcctcctcga ggcgcgcggc gatggcgctc atgccggccg cctcctccgg
    15121 gcagaagagc ggcccggagc agtatgcggt gtacatggcg tcgtgcggcg tttcgatcgg
    15181 catggcggaa gcccgtcacg ccgggggcgt tctgtcaagg cggatatcac gacgcgggag
    15241 gcgccatcag cccaatttgt tgtggcaatt attccgcggg gatgtatacg tggcagcgtg
    15301 agcgcgcacc gtccatgaaa aatatactca tctattccga tacgctcgcg tcgttcgcct
    15361 acggcgccta tcacccctac aagccggagc gggcccgtca gttcaaggag ctcgccgcgc
    15421 gctacgggct gctgtacgag gcgaaccagg agatcgtgga gccaaagccg ctcgatgaag
    15481 agcttctgta tctgttccat gaccgggact acatcgacct cctcaaggcg agcgagcgcg
    15541 gtgcccccac cgtcgagatg ctggaggcgg ggctgggatc gcccgacaat cccatcattc
    15601 cggggatgta cgagttcgcg ctgctcgccg cgggcggcac cctcctcggc gcgcagctcc
    15661 tggacgagga cgcggcgcgg ttcgtgttca atccgtcggg ggggttccat catgcgggcc
    15721 gcgcgcacgc cgagggcttc tgctacatca acgacatcgg cgtgacgatc gagcacctca
    15781 tccggcgggg gcggcgcgtc gcctacatcg acatcgacgt gcaccacggg aacggcgtgc
    15841 aggacgcgtt ttacgattcc gaccgcgtgc tcacgatatc catccacgaa tcgggcgtga
    15901 gcctctaccc gtggtccggg ttcgagaacg agatcggcga gggggcgggc cgcgggtaca
    15961 atgtgaacgt cccgctgctc ccggagacgg acgaccacgc ctactggtac gccttcgagt
    16021 cggtggtgcc gccgctggtg acgcacttcg cgcccgacat cgtcttcgcc gagatcggcg
    16081 gggacctgca ccgcgacgat ccgctggcga acctgaacat caccaccgag gtgtggtgcc
    16141 gcgccgtggg cgccatcaac gcgctcgcgc cgcggatcat ggcgctgggc ggcggcgggt
    16201 acaatcccca caagaccgcc gcgctgtggg cgctggcatg ggccatcttc tgcggcctca
    16261 cgccctcgga cagctatgcc ggcatcgtgg gggggatgat gtacggcccg gaggcgcagg
    16321 ccggctcatt gcacgacgaa ccgtcggcgg cggaatcctg cgcgctcaag ccggcccggc
    16381 acgcccacga cgttgtgcga catataaggg aaaccgtttt cccgctgcac gggatcgcgg
    16441 cctgagggtt cgggccgcgg accgatgcgg gacgcggcga ttcaatttca caaggaagcg
    16501 attatgccaa aaaaagttca tccgacagca accgtcaggg gcgacgggat ccccctgatc
    16561 atcgagaaaa aatcgaagct ctatttccag aacgtctccg attcggtcgt cttcatcttc
    16621 aagacgctcc tgggaatcga cgccacgccc aagaagctct ttcggcggcg gctcacctcg
    16681 cttcccaacg acatcatcat caagctggat ttcgcggccg acctgacggg ccatttcgcc
    16741 ttctttctgt cgctcaagac cgccttcgag atctgcaacc gcctggtgcc gggcatcggc
    16801 taccagtatt tcgacaagga ccacctcgac gtcatcgggg agatgggaaa catgatttcg
    16861 ggcaacgcga tcggacgcct gcagaacatc gacagcgcca tcgacctgtc gatcccgtcc
    16921 atgacggcct tccacaccat cctcgagtcg gagagccgct actggagctt ctcgggtaac
    16981 ttcgacgtgg acatcggcca ggtggggatt cttcttgcaa taaggaagga caagtaacgc
    17041 ggaatgccat cctctcccgt cgacgcgccc cccatttacg catgatgaga tcacgctacc
    17101 cgcgcgtgtg ctcgcagttt cgctcgctac tttcttttgg tcggccaaaa gaaagtagcc
    17161 aaagaaaaac cgctcgctcc atggccgcga gggtccaaat agacttcgta tcacaccact
    17221 ctcgcaaacg ggcatcgaac tgctggcggt catcctcggt ctccgactgc gcgaaaacct
    17281 gcgacctcgg cccgcatggc ctcctggcgg caaaaagagt attcgccgcg aagaagatcg
    17341 tattggacca atgatcaacc gcggaaccat caatagtata ttcgaacggc tgcgtgcgga
    17401 actgggcgat gcgtccgtta cccgcgatgg cgacatcctt gccgcctatt cgcgcgacga
    17461 gacctccgac ctcgccgccg cgcccgacat cctggtgcgc gcgcgcagcg cgaccgacgt
    17521 cgccgcggtg atgcgcgcgt gcaatgaacg ccgcgtcccg gttgtgccgc gcggcgcggg
    17581 gaccggcgtg accgggggcg ccgtcgcgac cgcgggcggc gtggtgctct cgctggagcg
    17641 catgaaccgc atcatcgaga tcgacgcgaa gaacatgatc gcggtggtgg agcccggcgc
    17701 gatcacgggc gagatccagc gctccgcggc ggcggcgggc ctcatgtatc cgcccgaccc
    17761 ggcgagcctg gattcctgct ccatcggcgg gaacatcgcc gagggcgcgg gggggccgcg
    17821 cgccgtgaag tacggcacca ccaaggatta cgtgacgggc ctggagttcg tgacgccgga
    17881 gggccacatc atcacggccg gcgggaagta cgtgaagaac gcgaccggat acaatctcgt
    17941 gggcgcgatc atcggctcgg aggggacgct cgtcgtcgtg acgaagatat tcctgaagct
    18001 cctcccggcg ccgcgcgcgt cgctcgatct cctggtcccg ttcgattcgc tggattccgc
    18061 cgttgatacg gtcaccgcga tcctgcacag cggcgtgttg cccgccgcga tcgagttcat
    18121 ggaggccgac gccatcgacc tggtggcgac gttcctgcac accgccatgc ccttccccga
    18181 tgcgcgggcg catctcctca tccagctcga cggcgcctcg accgccgagg tggaacacgc
    18241 gacggagacg atcgcgcgcg ttctgccggt ccccgcggag cgcgtgatcg cggcgcaaga
    18301 ccccgcccag cgcgagcgca tctggaaggc gcgccgctcc atccgcgagg ccatccacgc
    18361 cgagagcccg gtcttcctgg ccgaggacag cgtggtgccc cgcaacgaga tcccggcgtt
    18421 tctcgcggac gtgaaagcgg agctgcagag gcgcgccctc cgctcggtga tgttcggcca
    18481 cgccggggac ggcaatgtgc acatcgacgt cctcaagggc gccatggacg acgcgcaatg
    18541 gggggcgctg gtcccggaac tcaaggggat gatctatcgc gcggcgatcg cacgcggcgg
    18601 gaccatcacc ggcgagcacg gcaccggctt cacgcgaaag cgctatctgg gcctcgccta
    18661 cagcgcggag gccatcgaac tcatgcggcg catcaagacc gcgttcgatc ccaacggcat
    18721 cctcaatccg gggaagatca ttgacggtta gcggcggaca cacgaacgac ccggcgaatc
    18781 aacgcctatc ccaaaaagta tttttctaaa aatttctggg gcgacaccac gcgcctgtca
    18841 ttcgccgcca gaaagtgttt tacattgccg gtaacgagaa attttgcgct ggatgcagct
    18901 aatacctcgt agaacatgag atcgtcgggg tcgggaagca caagcgcacg cgggaccgga
    18961 acaatccgtt caccggcatg agcgataaac agcagaagtg actcaacgtc atccggatgg
    19021 aaccggaatc gctcccgcag cagcaccccg cgatattcaa tgaaaatgcg atcatcataa
    19081 cacacgacga cacgcccgtc gaggacgaga ttcagaatat cggccggttt tccgcgggga
    19141 ttcaggatcg ccgaaacgag aacatttgta tcaagcacga tcctcatgtg ccggagcgat
    19201 agcgatgaat ctcccggcta atttcctcgt cggacaatga aatcgtatca ccatcatcag
    19261 ccgtccgtcg catcgaccga agagcctcct cggcgcgcgc ttttcgaact gcacgcaaca
    19321 gctcctcaag accgctctcg aggatgggag taatgagccc gatcggtttc ccgttattgg
    19381 tgatgacaag ctcacgctcc ttcttcagat cacgccaaat tgccgctggc tttgttcgta
    19441 aatcacgaac tgtaataaat ttcataccca ttcaccccga ggaaatataa taccatattg
    19501 tcatccaatc gtcaacaaat atttaaattc aacgcatcaa gaccgcgttc gatcccaacg
    19561 gcatcctcaa tccggggaag atcattgacg gctagcggct cgagcatgca ccattccagc
    19621 gttatgccct gcggttttat tgttgaataa ttttcatggc cggctatctt ctttaaaaat
    19681 ggcagattta cattcatccc acatctaaac gagtgacgaa aacagcatga acacaccctc
    19741 atacaccacc gacggcctgc gcgattttct caccgcacac ccctggccgg aggagtgggc
    19801 gcatcgggct ttgccggtgg aatattattt cagcttcacg cttcccgcga cgccagcgga
//
LOCUS       DAORHF010000041        19261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_1977_length_19261_cov_5.122045, whole genome shotgun sequence.
ACCESSION   DAORHF010000041 DAORHF010000000
VERSION     DAORHF010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19261
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1977_length_19261_cov_5.122045"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PKJ16_05545"
     CDS             complement(<1..736)
                     /locus_tag="PKJ16_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46485.1"
                     /translation="MTLKIISALTPLRIITCFFFLCTYALPGCLSKNVALDRFEITPE
                     AYTAADVYQSMTIDDRGMPKYDGKLKKTPDAIGSTFVLVFKDEKNRPSKALSMMIVKH
                     QEKADLARPIKVVFEWTQKGFETGISLWSGGGGGSAGGSSSGSCGSGCISSPGGGGKD
                     AIAILAILGIVVALVAVVFVSTPIIGGTGGFVVGLSQSPVEATKEIRKTFTDRIPLAL
                     VYLSYHYEGAGRLVGINQYIASNPAKL"
     gene            complement(792..1478)
                     /locus_tag="PKJ16_05550"
     CDS             complement(792..1478)
                     /locus_tag="PKJ16_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNV46486.1"
                     /translation="MNILIFGPNGSGKGTQGTLVQKEYNVVHVESGVIFRENIKKGTE
                     IGKKAKEYIDRGELVPDGITIPMIVSRLQEPDCKNGWLLDGFPRSIEQAKALDEAVEK
                     AGMKLGCVIEIVLPREIAKNRIMGRRLCANDNNHPNNIFIDAIKPTQKGDDFVCRVCG
                     GALSARSDDQDEVAIGKRHDIYYDTKTGTMAAVNYFKDVAAKKGIKIIELDGKPGVKE
                     VTEELMKKLK"
     gene            1666..2172
                     /locus_tag="PKJ16_05555"
     CDS             1666..2172
                     /locus_tag="PKJ16_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46487.1"
                     /translation="MDNALSIGIRGGECVVVAEGEIRAGECFALGEVLPRYLDNANAP
                     TTVYLDLARCTYMDSTFIGFIVGLAKRSGAGGVVIANPSAAASEALRKLSALSAIEIK
                     PAITLPDVPTFRIARDTTAFGSIRNIELVFDAHRTLASLSEENRREFESLLHELSRVI
                     ESKRGAAS"
     gene            2169..2756
                     /locus_tag="PKJ16_05560"
     CDS             2169..2756
                     /locus_tag="PKJ16_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46488.1"
                     /translation="MKGELRRQQILDSALRLFAEKGYYETHIDDIIRDLRIGRGTVYQ
                     YFANKDDIFFQLLEDYYHRWKQVVAISDEERKINDPVAYLRMRIRRTLAFFAGEREFT
                     RIYLRIAPGINKDIEPLIERIETMVTDVVRQDLELARRFNHLRDDIDISGAANLLIGA
                     LHRTAYYDFIQTETDADIGVTAENFVRVFTAGIFK"
     gene            complement(2794..3252)
                     /locus_tag="PKJ16_05565"
     CDS             complement(2794..3252)
                     /locus_tag="PKJ16_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46489.1"
                     /translation="MSVKTTLYVHENTLELIHDACAITGKKRTEVIVMLLTRSLREAS
                     RDVTIGKSVRYQARDRAGRWRTIHVSFREDEADYCKDLRNFLKRSDSLILALAARAYL
                     RGCIKKSCFDGWGDNYRFSNYIISREESEGLIFWKICWGFPKTPEAHFRL"
     gene            3374..3841
                     /locus_tag="PKJ16_05570"
     CDS             3374..3841
                     /locus_tag="PKJ16_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV46490.1"
                     /translation="MENIYQSECQLRIKADPSTCYETLCDCSRFPDWFDCVRTVDVVD
                     RHEDGKPRRVRYQFDAVLRQGFDVILSKQYDDANRTITFVMRDEDRREVSGYCTVEPG
                     DRGGARATFYCRAALDFPFPPMVSSMFEERFAQAVLGYIRDAAEGRSCMAVAM"
     gene            complement(3908..4837)
                     /gene="dusB"
                     /locus_tag="PKJ16_05575"
     CDS             complement(3908..4837)
                     /gene="dusB"
                     /locus_tag="PKJ16_05575"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HNV46491.1"
                     /translation="MLILENIDLASPLVLAPMAGYTDAPFRRIARRHGAALVFSELVS
                     AEGIVRRDRKTAGYTRFTDDERPLGIQLFGMEPAVMGEAARIVSELGPDIIDINMGCC
                     APKVCHNGSGASLLRDPALTGRIAAAVVAGASVPVSAKIRLGWDAESRTYRELVRILE
                     DAGVRMITVHGRTASQRFGGVADWDAIREIRENASVPVIGNGDIATHAQALERHAASG
                     CAAVMIGRAAVGNPWIFSGGSPSVGDIVAQIMEHLDAMIDLYGEYGIILMRKHIVKYV
                     HHVKNASKLRNELVHATRRDEIISILARISDDG"
     gene            4999..7071
                     /locus_tag="PKJ16_05580"
     CDS             4999..7071
                     /locus_tag="PKJ16_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46492.1"
                     /translation="MAVKRFIVECDLSREGDLLRAIEACGCAQRPFVPDDAVTPGALG
                     EYSTDIRMLRKSEAHFRALLEYMSVYVYSVVYRAGSVISSYHSPRCEQITGYTPEEFS
                     ADHDLWYAMILPEDRPLVDRFLADLSASNGRPVSVEHRIRHKNGEVRWILNTCTVRIN
                     VEKDITRRDGSIIDVTRRKAAEEALRESEESNRRIIDTALEGIIRMDTEHRIVYTNRR
                     ITEMLGFDPEEMLGRAVEAFLFPDDVGDHRGKMARRREGHGERYERRYRHKDGRAVWT
                     IVSAVPLVDADGAFIGSFAMVTDITERKLAEDQLRKLSRAIEQSPASVVITDKTGTIE
                     YVNPKFTRLTGYTLEEAIGENPRILKSGEQPSEYYRNLWKTILAGEEWQGEFHNRKKN
                     GELYWEVASISPIRDAKGNIANFIAVKEDITRRKEAEEALRVSEEALKKRNQIFEKDL
                     KLAQIIQSALLPRELPSNGRIHVEYRYQPMDEVGGDFFSFYQQPGGNLGVFIGDVAGH
                     GISSALFLSLVKSVTERAVKDCFDAPAAYLDSINRELIGYMPSYFITGIYAYFDFESD
                     PEGCRFVFSSGGHPGPVVVRSDGAGEIVNSGGTIIGSFDEARYSETSLSLRRGDRVFL
                     YTDGIPETANAAREIIGFDSHLVDLLCAARRDELGSHLDAILGRVAEFRGAFPVEDDI
                     IIIGFEVA"
     gene            complement(7049..7528)
                     /locus_tag="PKJ16_05585"
     CDS             complement(7049..7528)
                     /locus_tag="PKJ16_05585"
                     /inference="COORDINATES: protein motif:HMM:NF020091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HNV46493.1"
                     /translation="MLTYEIQDASRALAIARSLKSYASPFAVVGGYKLIDNGVEPEAT
                     VQIEANGTLIHEASTGKGPVDALANVLKKALTPLFPEIEGVKLVDYRANIMDAKRGTS
                     TDVEVTIIFTDGDRVWRVYSLSENINAASFNVLIDGFEYAILMKRARDGSVTPPQNQ"
     gene            complement(7543..8028)
                     /locus_tag="PKJ16_05590"
     CDS             complement(7543..8028)
                     /locus_tag="PKJ16_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46494.1"
                     /translation="MKIFHNILIVIEILGIAILISCSGESAQDTIKFTVYSQGGSYQE
                     FSGNYQINGGTPGALASNASSNSVCYYDKEITSDDFSEDLLDTIDITAALTTTSIPAT
                     YPTMSIKVYRLDKSGNIKKVKETSNTSSQVSITYKYDEENSSSSSDSSTSKSSSSSSS
                     E"
     gene            8233..9060
                     /locus_tag="PKJ16_05595"
     CDS             8233..9060
                     /locus_tag="PKJ16_05595"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HNV46495.1"
                     /translation="MKKTELAQYARGVFINMPFRQIEEKYRPLIERYRFDLEIGIDHI
                     ALDTFPREVFRETAARLKDLGLAFTIHAPFNEIFLGAPDRLVRDAARQRLDMAFGIIE
                     HFLPVTSVVMHLNYEDRRFGFVYDDWFGHVVPAIRDFAGRCAEMRTTLMLENVYEETP
                     DAMAAVFDALSGAGVGHCLDAGHLSAFSETSLPDWLAVMGARVRQFHLHDNDGTSDAH
                     APIGTGAIDFGLIRDFIARMDVRPVITLEPHSEEDLWATLAGYEASGVRAAVQGLTA"
     gene            9130..9813
                     /locus_tag="PKJ16_05600"
     CDS             9130..9813
                     /locus_tag="PKJ16_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV46496.1"
                     /translation="MKELLKNAISRGQAALSEYDSKRVIATAGVSVSRNGLATGKEEA
                     VRIARELGYPVALKGCGHALTHKTELGIVKLGLGDDGAVASAYDEIVAKGLPMDGVLV
                     DSMLPGDGREFVMGMMRDPQFGPCVMFGLGGIFTEALRDVSFRVAPLTELDAGEMLDE
                     IRANRLLGGFRGSPPVDRTALVRALVGIGRLGLEHPEIAEIDVNPVLVHAGTPVAVDA
                     LVVLNAQAR"
     gene            9895..11097
                     /locus_tag="PKJ16_05605"
     CDS             9895..11097
                     /locus_tag="PKJ16_05605"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV46497.1"
                     /translation="MGGLKLKHNKIGIIFSGGPAPSANTVISSTAMNFLDHNIPVIGF
                     IRGYEFMQDFNIHYPRLRKGVHYEEITTEISHYRNDRGIYLRTSRANPGKNVKTIEDL
                     RDPGKNVLLRNILDVCEYLKIGALISIGGDDTLKTANFLWLMGLPVIHIPKTIDNDYY
                     GIPWTFGYWTAVDAAQRVMLNLKADAQATDSFFIVELMGRKAGWITYAAGIAAEAILM
                     ISAEDIEGDEMNVDDIVEKITDVIIARENNRKPYGVIAMSEGLADKLPKNLKPTETDR
                     HGNVMFAKANLSQMLADRVCALYRNKTGLEVKVTPKQIGYETRAAAPISFDVVLGSML
                     GYGAYKFFSEGTFGVMVSVTDNFELKAVPFSELIDPDTLRTRLRGVPQGSDFYTLKER
                     LSFVKFWE"
     gene            11140..12339
                     /locus_tag="PKJ16_05610"
     CDS             11140..12339
                     /locus_tag="PKJ16_05610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV46498.1"
                     /translation="MHSLLEKSDIIGSKEQRRLLERLVESGQEYLGVFDLVPHGVCVL
                     VDRSIVHCNRAGRAVFGAADPDVVTGRSIIDIVDVAERRRIAEWLKKALDLGVQLPPA
                     EFSFVRSDGGSAAIEARALSFSMRGRGAIMMVFRDVSDERRNRRALRESEERFRDISC
                     SIGDVIWEVDPDWCYTYASGRVEELLECRSEEIVGRSFFEFMPEDERLRVQALCMGAS
                     ARRVPLVDVERLMRTGSGKKIYVQTNAVPVEAPDGTVGGYRGVDRDVTDRRLARDVIR
                     DSGEKYRAVLEAISDPLYISSAGLRVSYLNPAMIARVGANAVGSACHEAIHGLPERCP
                     WCVSGEALAGRCARFEVASPLDNRIYVVSHSPIRHTDGSVSTIHILADITERRLAGAD
                     GRGGGQR"
     gene            12391..12861
                     /locus_tag="PKJ16_05615"
     CDS             12391..12861
                     /locus_tag="PKJ16_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46499.1"
                     /translation="MWRALLGAALAVSLAGCTRTDSPEGTLAALHELAEKGAYAQMRT
                     LLTPESVAAIGSIESAHPGFDGLDADFRRKFPRGARWGVIEKKASGDTVTLRLRCEKH
                     PVQNIVGYEMDLAMRRSGGAWRIDLRGDLEALSGRYDAYRKMIQNPAALRRFLK"
     gene            12937..14502
                     /locus_tag="PKJ16_05620"
     CDS             12937..14502
                     /locus_tag="PKJ16_05620"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46500.1"
                     /translation="MRVLLINPPYPFEESPTPPFGLISLAAYLIEQGAEVRIEDYIVT
                     PYSADRARRVIGEFRPDVIGATAVTMNVKPALSILADCKEAAPGAVTVMGGPHVTFDA
                     DAVLREYVHVDYIVRGEGELTFHEMLSLVEAGRPLDGVLGISYRDGGVVRHNPMRPFI
                     EDINMLPYPARQLISLGKYRALGFPINMVTSRGCPHKCIFCVGNRMVGNKVRYFDVTR
                     VVDEFELLATMGFKQINIVDDLFTSHKRRCIEICDEIVRRGIRHPWTAFARVDTVSED
                     LLAALKRAGCTTLCFGIESGNQEILDLVKKKITLEKARRAVELCRAAGIDPMTSYILG
                     LPGETPETVRKTMEFAKGLSSNYGFHILAPFPGTEVREKSDEYGMRILTSDWDLYDAN
                     RSVAETEFISHEEIDRIVNEFNGGLRRIVIDMLASRDRGETLPPQHAQMVQSLVNFLF
                     ARDLIVNELVENYNGAGNGCDRDAILAGFAAHVSGAIGMPETDVRAHLERLVGANCIA
                     VCGSGSGNSLAWN"
     gene            complement(14513..15226)
                     /locus_tag="PKJ16_05625"
     CDS             complement(14513..15226)
                     /locus_tag="PKJ16_05625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV46501.1"
                     /translation="MTTPAWQYDEMTQIGTDYASEDEVRQYDERMGRFRDRAADADAI
                     MEAIGLDHDHIVMEIGCGTAEFAIHASERCERVIAIDISPVMLEYARTKAAARGRANI
                     DFIHAGFLGYKHAGAPLDAIVTELALHHLPDFWKLVALHRVAGMMRSDGMLYLKDVVF
                     SLNLEKYEKTLTYAVEDLRKAAGDDTARNFERHIRQEYSTYDWIMEEMLYRAGFDIID
                     AAYGDHCLATYLCRKGERR"
     gene            15312..15773
                     /locus_tag="PKJ16_05630"
     CDS             15312..15773
                     /locus_tag="PKJ16_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46502.1"
                     /translation="MSVDTTIYVHETTLQLVDVAIARTGKKRNHIIIALLERAMADST
                     MRIKTGCAVRYQERDCPARWRTVHVRLQAEEAEYFTDMRNFFKRSVSLLLAEAARAYL
                     AELVENAHEAKMDNYPFSGYSLSREVKDGLVLWHICWGSSLNPSGGLPRRP"
     gene            complement(15836..16645)
                     /locus_tag="PKJ16_05635"
     CDS             complement(15836..16645)
                     /locus_tag="PKJ16_05635"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV46503.1"
                     /translation="MNVPDSAFLDEFVGVWDDGAKRDPAFITTGVMAILPDYYGDIAA
                     IPPPFPVTPGERVLDLGCGWGRMLNNVCALGANAVGMDISSDMLAAARGYINTNKRNA
                     SFVRGDGTRLPFADNSFDTVYSLLVLQHLSKENGRAVFREIHRVLKPGGHAHVWVPGR
                     FAPENLLFAFLQFVSIHLFRHKDPIRMRFYRIGEARRMCAGLFSSFDATGHEFRPPWN
                     LHTKWTWHKILIPRRFHKGLRRISDRIENAANSRFRFLRRFGVVTIVRVVK"
     gene            complement(16629..18137)
                     /locus_tag="PKJ16_05640"
     CDS             complement(16629..18137)
                     /locus_tag="PKJ16_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV46504.1"
                     /translation="MKNMFNPIIFTRRRIIAIAIGIVGAAVIYSTINFGVARYFGNSE
                     KPRATAPTDAGAARRYDAIYRTLPAGFTHCGRMLFYTAPADTSVRAVADLAVPFTSFY
                     KAYRLRDAIRSFNAISGDEIEKGQMVFIPHSLPALMPDMRNSRKPPLIFARGLYYTGS
                     SAGRESLLENIDEYKELGINCIVFDAKDVCGIVNYKSRVPEAIETDAHGKRTIDDIDQ
                     LIRILKEKKIYVIARLALFHDQHMFKHNPDWAIQSKSGDGPWRAEAKEKWLDPTNKAA
                     QDYNLALAVELAEKGVDEIQFDYIRFPTTGKQGDAEFAYDFGTMSRDDTITAFLKRAH
                     EEIAKRNVLLSIDIFGVVAWGKEIDIIKTGQRIERLAEHCDIISPMLYPSHFNDDFDG
                     YARPGDHPYYFIHEGCKKVIALAKGKTVRPWLQAFRWRVSNYNEHYIIKQVKAVKDSG
                     AYGYLFWNAANDYRVVLRAMESIDTETALAGERSASPLAACEVRNEHHERPR"
     gene            18202..18996
                     /locus_tag="PKJ16_05645"
     CDS             18202..18996
                     /locus_tag="PKJ16_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV46505.1"
                     /translation="MSGTIIIIRGLLGKCFGSFEGVRKFFAFVRSIFVSFRSLRYLRL
                     RSLVAIAVNQTRFTGYDALPIIVVISLILGATVIIQATQNFPKFGIEGFIGNLLVIII
                     ARELGPLATAMVVMSRSGSAIATEIATQKQGREIQSLELMGIDSRLYIVIPRIAASIA
                     SILSLIIIFDIVAFFGGYLISLSTVFIPIDIFLQTLLDSMSADDLIITLVKSVVYGTT
                     IPLVCCYYGFMPRSKFEIPIYVSRAVMRTLLILFVVNALISALFYL"
     gene            19000..>19261
                     /locus_tag="PKJ16_05650"
     CDS             19000..>19261
                     /locus_tag="PKJ16_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46506.1"
                     /translation="METTDIILRLENVVYDADEFAPLTGITFELAWGEDLVVFGPEDS
                     GLNTLCPLIAGLALPAAGEIYFKSAPIKGFDYFQMHRYRKELG"
BASE COUNT         3589 a         5628 c         6063 g         3981 t
ORIGIN      
        1 ccagcttggc cggattcgag gcgatgtact ggttgatgcc taccaggcga ccggccccct
       61 cgtagtgata tgacagatac accagcgcga ggggtatgcg gtccgtgaag gttttgcgta
      121 tttcttttgt ggcctccacc gggctctggg agaggccgac gacaaacccg ccagtgccgc
      181 cgatgatcgg ggtggaaacg aatactacgg cgacgagggc cacgacgatt ccgagaatgg
      241 cgagaatcgc tattgcgtcc ttccccccgc caccgggact tgaaatgcat cctgagccac
      301 aggagccgct tgatgatccg ccggcgctgc cacctccgcc cccactccac aggctgatgc
      361 cggtctcgaa gcccttttgc gtccactcga ataccacctt gatcggccgg gcgagatcgg
      421 ccttttcctg gtgctttacg atcatcatcg acagagcctt gctggggcgg ttcttctcat
      481 ctttgaacac gagaacgaat gttgatccga tcgcgtcggg tgttttcttc agcttcccgt
      541 cgtacttcgg cattccacgg tcgtcgatcg tcatggattg atagacatcg gcggcggtat
      601 aggcctcggg ggttatctcg aaacgatcaa gcgcgacgtt tttcgagagg cagccgggga
      661 gcgcgtacgt gcaaaggaaa aagaagcagg tgatgattcg caacggcgtc aatgcggata
      721 tgatcttcag ggtcatggca ttcagaaaaa gccggagggc tgcaccctcc ggcttttcag
      781 atcgcgtttg attacttcaa cttcttcatg agctcttcgg tcacctcttt cacgccgggc
      841 ttcccgtcga gttcgatgat cttgatgccc ttcttcgcgg cgacgtcctt gaagtagttc
      901 accgccgcca tggtgccggt cttcgtatcg tagtaaatgt cgtgacgctt gccgatggcc
      961 acctcgtcct ggtcgtcgga gcgggccgag agggcgccgc cgcagacgcg gcatacgaag
     1021 tcatcaccct tctgggtcgg cttgatggcg tcgatgaaga tgttgttcgg gtggttgttg
     1081 tcgttcgcgc acaggcgtcg gcccatgatg cggttcttcg cgatctcgcg gggcagcacg
     1141 atctcgatca cgcagccgag cttcatgccg gccttctcca ccgcctcgtc gagggccttg
     1201 gcctgttcga tggagcgggg gaagccgtcg agcagccagc cgttcttgca gtccggctcc
     1261 tggaggcggc tgacgatcat ggggatggtg atgccatccg ggacgagctc gccgcggtcg
     1321 atgtattcct tcgccttctt accaatctcg gtgcccttct tgatattttc gcggaagatg
     1381 acgcccgatt ccacgtggac gacattgtat tccttctgga cgagcgtgcc ctgcgttccc
     1441 ttgccgcttc cgttgggccc aaaaatgaga atattcatga ttccacctcg ttgaaatgat
     1501 atggacaatt tacggcacat taaaaatttt tgtatatata atgcaagaat aaattttcac
     1561 cagcgcgacg agccgcatca aggtgataaa aaactggttg cgtgaaaacg gcctcggcac
     1621 tagcgtacat ggggcgacca ttgaaacaga gggggcggca tatcgatgga caatgcgctc
     1681 agcatcggga tacgcggcgg ggagtgcgtc gttgtcgcgg agggggagat ccgcgcggga
     1741 gagtgcttcg cgctgggcga ggtgttgccg cgctatctgg acaacgcgaa tgcgccaacg
     1801 accgtgtatc tggatctggc ccggtgcacg tatatggact ccaccttcat cggctttatc
     1861 gtgggattgg cgaagcggag cggtgcgggc ggtgtcgtca tagccaaccc ctcggcggcg
     1921 gcgagcgagg cgttgcgaaa gctctccgcc ctgagcgcga ttgagatcaa gcccgctatc
     1981 accctgccgg acgtgccgac gttccgtatc gctcgcgata cgaccgcgtt tggttcgatc
     2041 aggaacatcg agctggtgtt tgacgcgcat cgtacgctcg cgtcgctgag cgaggaaaac
     2101 cggcgtgaat tcgagagcct cctgcacgag ctttcccgcg tcattgagtc gaagcggggg
     2161 gcggcgtcgt gaagggagaa cttcgccggc agcagatcct cgacagcgcc ttgcggctct
     2221 tcgcggagaa gggctactac gagacgcaca tcgacgacat catccgcgat ttacgcatcg
     2281 ggcgcgggac ggtgtatcag tatttcgcga acaaggacga tatcttcttt cagctgctcg
     2341 aggactatta ccatcgctgg aagcaggtgg tggccatctc cgacgaggag cggaaaatta
     2401 atgatcctgt cgcgtacctg cgcatgcgca tcaggcggac gctcgcgttt ttcgccggcg
     2461 agcgggagtt cacgcgcatc tacctgcgca tcgcgccggg catcaacaag gacatcgagc
     2521 cgctcatcga acgcatcgag acaatggtga ccgacgtggt gcgccaggac ctcgagctcg
     2581 cgcggcgctt caatcatctt cgcgacgata tcgatatttc cggcgcggcg aacctgctga
     2641 tcggcgccct gcatcggact gcatattatg atttcatcca aaccgagacc gatgccgaca
     2701 tcggcgtgac tgccgaaaat ttcgttcgcg tgttcaccgc cggcattttc aagtgaaacc
     2761 ccggcgttga tggatgattg cgggcctgtg cggttatagg cggaaatggg cttcgggggt
     2821 tttggggaac ccccagcata ttttccaaaa gatcaagccc tcggactcct ccctcgatat
     2881 gatgtaattc gaaaagcgat agttatcgcc ccatccgtca aaacacgatt tcttgataca
     2941 gccacgcaga tatgctctcg ctgccagcgc gagaatcagc gaatccgacc tcttcaggaa
     3001 attccgcaag tccttacagt agtccgcctc gtcctctctg aaactcacgt ggatggtgcg
     3061 ccagcgcccc gcacggtcgc gcgcctggta tcgaaccgat ttcccaattg tcacgtcacg
     3121 gctcgcctcg cgcagggatc gtgtcagaag catgacaatg acctcggtgc gcttttttcc
     3181 tgtaattgca caggcgtcgt gaatgagttc cagggtgttt tcatgaacgt acagcgttgt
     3241 ttttactgac attttatctc catgtgattg gtttattgat aatacggatc atcaggctgg
     3301 tagttgaaaa aatattgaca aatgtgagcc cttatattaa tcagaaccga cgtgtcggtt
     3361 tttggaggac tccatggaaa atatatatca atctgaatgt cagttacgca taaaggccga
     3421 tccctccaca tgctacgaga ccctctgcga ctgttctcgc tttcccgatt ggttcgactg
     3481 cgttcgcacg gttgacgtgg tcgatcgcca tgaggacggc aagccgcgtc gcgtgcgtta
     3541 ccagttcgat gcggtgctgc ggcagggatt cgatgtcatt ctcagcaagc agtacgatga
     3601 tgcgaaccgg accatcacgt tcgttatgcg cgatgaggat cgacgcgagg tgagcgggta
     3661 ttgcacggtg gagccgggcg atcggggcgg ggcgcgcgcg accttttatt gccgggccgc
     3721 gctcgatttc ccgtttccgc ccatggtttc atccatgttc gaggagcgtt ttgcccaggc
     3781 cgtgctgggg tatattcgcg atgccgccga gggccggtcc tgcatggcgg tcgcgatgta
     3841 gctcccgccg ccgggcggga gcaatcatac ctgattcgtt tgagcgtgtc tgtgggccgc
     3901 gacaggatta tccgtcatcg ctgatccggg ccagaataga aattatctcg tcacgccgcg
     3961 tcgcgtgcac cagctcattg cgcagcttcg aggcgttctt cacgtgatgc acgtatttca
     4021 cgatgtgctt gcgcatcagt atgatcccgt actcgccgta caagtcgatc atcgcgtcga
     4081 gatgctccat gatctgcgcg acgatgtccc ccaccgacgg gctccccccg gaaaagatcc
     4141 acggattccc gaccgccgcg cggccgatca tgaccgccgc gcacccggat gcggcatgcc
     4201 gctcaagcgc ctgtgcatgt gtggcgatat cgccgttgcc gatcaccggc accgatgcgt
     4261 tctctcggat ctcccgaatc gcgtcccagt ccgcgacgcc gccgaagcgc tgcgacgccg
     4321 tgcgcccgtg cacggtgatc atgcggacgc ccgcgtcttc gaggatgcgg acgagctccc
     4381 gataggtgcg gctttccgcg tcccagccca ggcggatctt cgccgatacc ggcacgctcg
     4441 cgccggccac aaccgccgcc gcaatccgtc ccgtgagagc cgggtcgcgc agaagcgagg
     4501 cccccgagcc gttgtgacac accttgggcg cgcagcagcc catattgatg tcgattatgt
     4561 ccggcccgag ttcgctcacg atgcgcgcgg cctcgcccat gaccgccggt tccatgccga
     4621 agagctggat gcccagcggt cgctcgtcgt cggtgaagcg cgtatagccg gccgtcttcc
     4681 ggtcgcgccg cacgatcccc tcggcgctta cgagctccga gaagaccagc gcggccccgt
     4741 ggcggcgcgc gatgcggcga aacggggcgt cggtgtagcc cgccatgggc gccaggacca
     4801 gcggcgatgc gaggtcgatg ttctcgagta tgagcatttt tatgttgcga aaaacggtat
     4861 tgtatcggta cacgtcatat caacctcact gccgcatgag gtgtcgtttg caaccattaa
     4921 ttcagtggtg cggcggcgcg cggcgatacg tcgggcgttg ggcaaacgat caattgatca
     4981 cgaacgcggg gtttttgcat ggccgtgaag cggttcatcg tcgagtgcga tctgtcgcgg
     5041 gagggagacc ttcttcgcgc gattgaggcg tgcgggtgcg cccagcgccc gtttgtgccg
     5101 gacgatgcgg taacacccgg cgcgctcggc gagtacagca ccgacatccg catgctccgc
     5161 aagagcgagg cgcattttcg cgcgctcctg gagtatatga gcgtgtacgt gtacagcgtc
     5221 gtctaccgcg ccgggtcggt catctcgtcc tatcacagcc cccgctgcga gcagatcacc
     5281 ggctacaccc ccgaggagtt ttccgcagac cacgatctct ggtacgcgat gattcttccc
     5341 gaggaccggc cgctcgtgga ccgtttcctt gccgatttaa gcgcaagcaa cggccggccc
     5401 gtttccgtgg agcaccgcat tcgccacaag aacggcgagg tccgctggat tctgaacacc
     5461 tgcacggtca ggatcaatgt ggaaaaggac atcacgcggc gcgacgggtc catcatcgac
     5521 gtgacgcgcc gcaaggccgc cgaggaggcg ctccgggaaa gcgaggaaag caaccgccgc
     5581 atcatcgata ccgccctcga gggcatcatc cgcatggaca ccgaacaccg gatcgtctac
     5641 accaaccggc gcatcacgga gatgctggga ttcgatccgg aggagatgct gggacgggcc
     5701 gtggaggcct tcctcttccc cgacgatgtg ggcgatcatc ggggaaaaat ggcccgtcgg
     5761 cgcgagggac atggggaacg gtacgagcgg cgataccggc acaaggacgg ccgggccgtc
     5821 tggaccatcg tctcggccgt tccccttgtc gatgcggacg gggcgtttat cggatcgttc
     5881 gccatggtga ccgacatcac cgaacggaaa ttggcggagg accagctgcg caagctctcg
     5941 cgcgccatcg agcagagccc ggcgtcggtc gtcatcaccg acaagaccgg cacgatcgaa
     6001 tacgtcaacc cgaagttcac ccgcctcacc ggctataccc tcgaggaggc catcggggaa
     6061 aacccgcgca tcctcaagtc gggcgaacag ccgtccgagt actatcgcaa tctctggaag
     6121 accatcctgg ccggggagga gtggcagggc gagttccata accggaaaaa aaacggcgag
     6181 ctctactggg aggtcgcgtc catatcgccc attcgcgatg cgaaggggaa tatcgcgaac
     6241 ttcatcgccg tcaaggagga tattacgcgc cgcaaggagg cggaggaggc gctgcgcgtg
     6301 agcgaggagg cgctcaagaa gcgtaatcag atcttcgaga aggacctcaa gctcgcccag
     6361 atcattcaaa gcgcgctcct gccgcgcgag cttccgagca atggccgcat ccatgtcgag
     6421 taccgctatc agcccatgga cgaggtgggc ggtgactttt tttcgtttta ccagcagccc
     6481 ggcggtaacc tgggggtgtt catcggcgat gtggccggcc atgggatttc ttcggcgctg
     6541 ttcttgtcgc tggtgaagtc ggtcacggag cgcgcggtga aggactgctt cgacgctccc
     6601 gccgcgtatc tggactccat caatcgcgaa ttgatcggct acatgccgtc gtattttatc
     6661 accgggattt acgcgtactt cgatttcgag tctgatcccg agggctgccg gttcgtcttc
     6721 tccagcggcg ggcatcccgg tccggtggtc gttcgctccg atggagccgg cgagatcgtc
     6781 aactcgggcg gcacgatcat cggttcgttc gatgaggccc gctactcaga aacgtcgctt
     6841 tcgctgcggc gcggggaccg cgtcttcctc tacaccgacg gcatccccga gacggccaat
     6901 gccgcgcggg agatcatcgg tttcgattcc cacctggttg accttttgtg cgcggcgcgg
     6961 cgggatgagc ttgggagcca tctcgatgcg atcctgggac gggtcgcgga gttccgcggc
     7021 gcgtttcccg tcgaggacga tatcattatt attggttttg aggtggcgtg actgatccgt
     7081 cgcgcgcgcg cttcatgagg atggcgtatt cgaagccgtc gatgagcacg ttgaacgagg
     7141 ccgcgttgat gttctccgaa agcgagtaga cccgccagac ccggtccccg tcggtgaaga
     7201 tgatggtgac ctccacgtcg gtgctggttc cgcgcttcgc gtccatgatg ttcgcgcgat
     7261 agtccaccag tttcaccccc tcgatctccg ggaacagcgg cgtgagggcc ttcttgagga
     7321 cgtttgccag cgcgtccacg ggccccttgc cggtggacgc ctcgtggatg agcgtcccgt
     7381 tcgcttcgat ctggaccgtg gcctcgggct ccacgccgtt gtcgatgagt ttgtatcccc
     7441 ccaccaccgc aaagggcgat gcataactct taagcgaccg agcgatcgca agcgcccgtg
     7501 acgcatcctg gatttcgtag gtgagcatgc agtacggtcc cgctactcgc tcgagctgga
     7561 actggaggat tttgaagtcg aggagtcgga actcgaggag ctgttctcct cgtcgtactt
     7621 gtaggttatt gatacttgcg atgacgtgtt ggaagtttct ttgacttttt ttatattgcc
     7681 cgacttgtct agtcggtaaa cttttatcga cattgttgga tacgttgccg gaatcgaagt
     7741 tgttgtcaat gcagcggtaa tatctattgt atcaagtaaa tcctctgaaa agtcgtcgct
     7801 ggtgatctcc ttgtcgtaat aacacacgct attcgatgat gcattacttg ctaatgcacc
     7861 aggagtccca ccgttaattt gataattgcc gctgaactct tggtaactgc cgccttgtga
     7921 gtatacagtg aatttgattg tatcctgagc cgattcgccc gaacaggaaa tcagaatggc
     7981 gattcccaaa atttctatga caatcaagat attatgaaat attttcatag tttctactga
     8041 ttctatagta tttttcgtac tttgtaaata atctacaaag tagtgttgcc gtatgtcaag
     8101 aaaaattaaa actcgaatcc gacaataata gtctttggcg gaaatttgta caattaaaaa
     8161 cgattgtaat cgtgaaaaat gtattttaca gcaaaaggcc tttttataat tgttaagact
     8221 gaataataca ccatgaagaa aaccgaactc gcacagtacg cccggggcgt tttcatcaac
     8281 atgccgttcc ggcagatcga ggagaaatac cggcccctta tcgagcgcta ccggttcgat
     8341 ctcgaaatcg gaattgatca tatcgcgctg gatacgttcc cccgcgaggt cttccgggag
     8401 accgcggcgc gtctcaagga cctggggctg gcctttacca tccatgcgcc cttcaacgag
     8461 atcttcctgg gcgcgcccga ccgcctggtc cgcgatgccg cgcggcaacg gctcgatatg
     8521 gccttcggga tcatcgagca cttccttccc gtgacgtcgg tcgtgatgca cctcaactac
     8581 gaggaccggc gcttcggctt cgtgtacgac gactggttcg ggcacgttgt tcccgccatc
     8641 cgggatttcg ccggtcggtg cgcggagatg cggaccacac tcatgctcga gaacgtctac
     8701 gaggagacgc ccgatgcgat ggccgcggtg ttcgatgcgc tttcgggcgc gggggtgggg
     8761 cactgcctcg acgcgggcca tctctccgcg ttttcggaga cgagcctgcc ggactggctc
     8821 gcggtcatgg gcgcccgcgt gcggcagttt catctgcacg acaatgacgg gacctcggac
     8881 gcccatgcgc ccatcggaac gggagcgatc gattttgggc tgatccgcga tttcatcgcg
     8941 cgcatggacg tgcggcccgt gatcaccctc gagccgcaca gcgaagagga cctgtgggcg
     9001 acgctcgccg gctacgaggc gagcggggtg cgcgccgcgg ttcaaggatt gaccgcatga
     9061 ggtttggccc atgaaaagca ttctggttgc cggcggtgcg tcacttattg caccccaagg
     9121 agtatcgaca tgaaggaact gctgaagaac gcgatttccc gggggcaggc ggcgctctcg
     9181 gagtacgatt cgaagcgggt gatcgcaacc gcaggcgtat ctgtttcgcg caacggtctt
     9241 gcgacgggaa aggaagaggc cgtccgaatc gcgcgcgagc tcggctaccc ggtggccctg
     9301 aagggctgcg gacacgcgct cacccataaa accgagctgg ggatcgtgaa gctcggtctc
     9361 ggggacgatg gggcggtcgc ctccgcgtac gatgagatcg tcgcaaaggg ccttccgatg
     9421 gacggcgtgc tggtggactc gatgcttccc ggcgacgggc gcgagtttgt catggggatg
     9481 atgcgcgacc cgcagttcgg gccctgcgtg atgttcggcc tgggcggcat cttcaccgag
     9541 gcgctgcgcg acgtgagttt ccgggtcgcc ccccttaccg agcttgacgc cggggagatg
     9601 ctggacgaga tccgcgcgaa tcggctcctg ggcggctttc gcgggagccc gcccgtggac
     9661 cgcacggcgc tcgtgcgcgc gctcgtcggc atcggtcgcc tgggccttga acaccccgag
     9721 atcgccgaaa tcgacgtaaa cccggtcctg gtgcacgccg gaacgcccgt cgccgttgac
     9781 gcgctggttg tcctcaacgc ccaggcgcga tagttttttt cttgaccgca ccgatttggg
     9841 cccgtaagat ccatccgtcg tgggagcata tcaatcagac ggggtgggcg gcacatgggc
     9901 ggactgaagc ttaagcataa taaaatcggc attatctttt cgggcggtcc tgcgcccagc
     9961 gccaacacgg tcatctcctc gaccgcgatg aattttctgg accacaatat tcccgtgatc
    10021 ggattcattc gggggtatga attcatgcag gatttcaata tccactaccc ccgcctgcgc
    10081 aagggcgtgc attatgagga gatcaccacg gagatttcgc actatcggaa tgaccggggg
    10141 atctatcttc gcacctcgcg cgccaatccg gggaagaacg tgaagacgat cgaggacctg
    10201 agggaccccg gcaagaacgt gctccttcga aacatcctgg atgtgtgcga atacctcaag
    10261 atcggtgcgc tcatctcgat cgggggcgat gatacgctca agacggcaaa cttcctgtgg
    10321 ctcatggggc tgccggtcat ccacattccc aaaacgatcg acaatgacta ctatggcatc
    10381 ccctggacct tcggctactg gacggccgtg gacgcagcgc agcgcgtgat gctcaacctg
    10441 aaggccgacg cgcaggcgac cgacagcttc tttatcgtgg agctcatggg gcgcaaggcc
    10501 gggtggatca cctacgcggc gggcatcgcg gcggaggcca tcctgatgat ctccgccgag
    10561 gacatcgagg gcgacgagat gaacgtcgac gatatcgtcg agaagatcac ggacgtgatc
    10621 atcgcccgcg agaacaaccg caagccctac ggcgtgatcg ccatgtccga gggccttgcc
    10681 gacaagctgc cgaaaaacct caagccgacc gagaccgacc ggcacggcaa tgtgatgttc
    10741 gcgaaggcga acctctcgca gatgctcgcg gatcgcgtct gcgcgctcta tcggaacaag
    10801 acgggcctgg aggtgaaggt gacgcccaag cagatcggtt acgagacacg cgcggcggcg
    10861 cccatcagct tcgacgtggt gctcgggagc atgctgggat atggcgcgta taagttcttt
    10921 tcggagggca ccttcggggt gatggtgtcg gtgacggaca acttcgaact caaggccgtc
    10981 ccgttctccg agctcatcga tcccgatacg ctccggaccc gtctgcgggg cgttccgcag
    11041 gggagcgatt tttacacgct caaggagcgc ctttcgttcg tgaagttttg ggagtgaccg
    11101 cagttgttgt gaaccggcat gcgggagacg gtggacggcg tgcattcact gttggaaaaa
    11161 tctgacatca tcggctccaa ggagcagcga cggctcctcg agcgcctggt cgaatcgggc
    11221 caggaatacc ttggggtctt cgacctcgtc ccgcacggcg tgtgcgtgct cgtcgaccgc
    11281 tcgattgtgc attgcaatcg cgccggtcgc gccgtcttcg gggccgccga tccggacgtc
    11341 gttacggggc ggtcgatcat cgacattgtg gatgtcgccg agcggcgtcg catcgccgaa
    11401 tggctcaaga aggcccttga tctgggcgtt cagcttccgc ccgcggagtt ctcctttgtt
    11461 cgatctgatg ggggctcggc cgcaatcgag gcgcgggcgc tgtcgttttc gatgcggggg
    11521 cgcggcgcca ttatgatggt gttccgcgat gtgagcgacg agcgccgaaa tcgccgcgcg
    11581 ctccgggaga gcgaggagcg ctttcgtgat atctcgtgca gcatcggcga tgtgatctgg
    11641 gaggtcgacc ccgattggtg ctacacctac gcaagcggcc gggtcgaaga gctgcttgaa
    11701 tgccgaagcg aggagatcgt gggccgaagc tttttcgagt tcatgcccga ggacgagcgc
    11761 cttcgggtgc aggcgctgtg catgggggcg tcggcgcggc gcgttccgct tgtggacgtc
    11821 gagcgcctga tgcgcaccgg ctccgggaaa aagatctacg tgcaaaccaa cgccgtgccg
    11881 gtggaggccc ccgacgggac cgtggggggc taccgcgggg ttgaccgcga cgtgaccgac
    11941 cgccggctcg cccgtgacgt tatccgcgac agcggggaga agtaccgggc cgtgctcgag
    12001 gccatcagcg atccgctcta tatcagttcg gcggggctgc gcgtatcgta cctgaacccg
    12061 gcgatgatcg cccgcgtggg ggcgaatgcc gtggggagcg catgccacga ggcgattcac
    12121 ggcctcccgg agcgctgtcc ctggtgcgtg agcggcgagg cgctcgccgg tcgctgcgcg
    12181 cgtttcgagg tcgcaagccc gttggacaac cggatctatg tggtgtcgca ttcgccgatc
    12241 aggcacacgg acggttcggt ttcgacgata cacatcctcg ccgacatcac cgagcggcgg
    12301 ctcgcgggcg ccgatggtcg gggcgggggg cagcgctgag gcgccatcaa cactatgcga
    12361 gagaggtgcg gtatgaacgg gcggtgtcgt gtgtggcgtg cgcttctcgg tgcggcgctg
    12421 gccgtctcgc tggcggggtg cacgcgcacg gactcgccgg aggggacgct ggcggcgctg
    12481 catgagctcg ccgaaaaggg cgcgtatgcg cagatgcgca cgctcctgac gccggagagc
    12541 gtcgccgcga tcgggagcat cgaatcggcc catcccggtt tcgacggact cgacgccgat
    12601 ttccggcgta agtttccccg gggcgcgcga tggggcgtga tcgagaagaa agcgagcggc
    12661 gatacggtca cgctgcgcct tcgatgcgag aaacatcccg tgcaaaacat cgtggggtac
    12721 gagatggatc tcgcgatgcg gcgctcgggc ggcgcctggc ggatcgacct gcgcggcgac
    12781 ctggaggcgc tctcgggccg ctacgacgcc tatcgcaaga tgatccaaaa tcccgcggcg
    12841 ctgcggcgct tcctgaaata ggcgtatttt ttatttacaa gttccgtggg tcggcgatag
    12901 tggccgcaat acccgttcgc accacaggat ccgcccatga gagtgctgct catcaatcct
    12961 ccgtatccct tcgaggagtc gcccacgcct ccgtttgggc tcatttcgct cgccgcgtat
    13021 ctcatcgaac agggggccga ggtccgcatc gaggactaca tcgtcacgcc ctattcggct
    13081 gatcgcgcgc ggcgcgtaat cggcgagttc cgccccgacg tgatcggggc gaccgcggtg
    13141 accatgaacg tgaagccggc cctctcgatc ctcgccgatt gcaaagaggc cgctccgggc
    13201 gccgtgacgg tgatgggcgg tccgcacgtg accttcgacg ccgacgcggt cctgcgcgag
    13261 tacgtgcatg tcgattacat cgtgcgcggc gagggcgagc tgacgttcca tgaaatgctc
    13321 tcgctggtcg aggccggaag gccgctcgac ggcgtgctgg ggatttcgta tcgggacggc
    13381 ggcgttgtgc gacacaaccc gatgcgcccg ttcatcgagg acatcaatat gttgccgtac
    13441 ccggcgcggc agctgatttc gctgggcaag taccgggcgc tggggtttcc catcaacatg
    13501 gtcacctcgc gcgggtgtcc gcacaaatgc atcttctgcg tgggcaaccg gatggtgggg
    13561 aacaaggtgc gttacttcga cgtgacgcgc gtggtggacg agttcgagct gctcgcgacc
    13621 atgggcttta agcagatcaa tatcgtggac gacctgttca cctcgcacaa gcggcgctgc
    13681 atcgagatct gcgacgagat cgtgcggcgg gggatccggc atccctggac ggccttcgcg
    13741 cgcgttgata cggtgtcgga ggacctgctc gcggcgctca agcgggcggg ctgcaccacg
    13801 ctgtgcttcg ggatcgagag cggcaatcag gagatcctgg acctcgttaa aaagaagatc
    13861 acgctggaaa aggcgcgccg cgcggtggag ctctgccgcg ccgcgggcat cgacccgatg
    13921 acctcgtata tcctgggact tcccggcgag acgccggaga cggtgcgcaa gacgatggag
    13981 ttcgcgaagg ggctgagttc gaattacggt ttccatatcc tcgcgccgtt tcccggaacg
    14041 gaggtgcgcg agaagagcga cgagtacggc atgcgcatcc tgacgagcga ctgggacctc
    14101 tacgacgcga accgctcggt ggcggagacg gaattcatct cgcacgagga gatcgaccgc
    14161 atcgtgaacg agttcaacgg cggcctgcgc cgcattgtga tcgacatgct cgcctctcgc
    14221 gatcgcggcg agacgctgcc cccgcagcac gcgcagatgg tgcaaagcct ggtgaacttt
    14281 ctcttcgcgc gcgatctcat cgtgaacgag ctggtggaga actacaacgg cgcggggaac
    14341 gggtgcgatc gtgacgcgat actcgccggc ttcgccgcgc acgtttcggg cgctatcggc
    14401 atgcccgaaa cggacgtgcg cgcccatctg gaacgcctcg ttggcgcgaa ctgcatcgcc
    14461 gtgtgcggca gcgggtcggg gaactcgctg gcgtggaact gacggcgcgc gcctatcggc
    14521 gctccccctt tcgacagaga tacgtcgcca ggcagtggtc gccgtacgcg gcgtcgatga
    14581 tgtcgaatcc cgcgcggtag agcatctctt ccattatcca gtcgtaggtt gaatactcct
    14641 ggcggatgtg ccgctcgaag ttgcgcgcgg tatcgtcccc ggcggccttg cggaggtcct
    14701 ccacggcgta ggtgagcgtc ttttcgtact tctccagatt gagcgagaag accacatcct
    14761 tcagatagag catcccgtcg ctgcgcatca tccccgcgac gcggtgcagc gcgacgagct
    14821 tccagaaatc tggtaaatga tgcagggcga gctccgtgac gatcgcgtcg agcggcgctc
    14881 cagcatgttt gtagccgaga aagcccgcgt gaatgaagtc gatgttcgcg cgcccgcgag
    14941 cggcggcttt tgtgcgcgcg tattctagca tcaccggcga gatgtcgatc gcgatcactc
    15001 gctcgcagcg ctccgaggcg tggatcgcga actcggccgt cccgcagccg atctccatga
    15061 cgatatggtc gtggtcgagg ccgatggcct ccattatggc gtcggcgtcg gcggctcggt
    15121 cgcgaaatcg ccccatccgc tcgtcgtatt ggcgcacctc gtcctcgctc gcgtaatcgg
    15181 tgccaatctg cgtcatttcg tcgtactgcc aggccggtgt ggtcatggtg cgatgctccc
    15241 gggtgcgcga aaataattac aatcgtcctt ccgcgttcgt attaatagta gagcagaaag
    15301 cgggggttga tatgtccgtc gatactacga tctatgtgca tgagacgacg ttgcagcttg
    15361 tcgatgtcgc tatcgcgcgg accggcaaaa agcgcaatca tattatcatc gcgcttctgg
    15421 aacgggcgat ggccgatagc acgatgagga ttaaaaccgg ttgcgcggtg cgctatcagg
    15481 agcgcgattg tcccgcgcgg tggcggacgg tgcatgtgcg attacaggcg gaagaagcgg
    15541 aatatttcac ggacatgagg aattttttca agcggtcggt ttctttgctg cttgcggagg
    15601 cggcgcgggc atacctcgct gagcttgttg agaatgcaca tgaggctaaa atggataact
    15661 atccattctc gggctattca ttatcacggg aagtgaagga cgggcttgtt ttgtggcaca
    15721 tctgctgggg ctcgtcgctc aacccgagcg gaggtctccc acggcggcca taacttggac
    15781 acgaaaaacc aaaagcacga tcacgcaacc gcccgagaag ccgttgcgca tgtccctatt
    15841 ttacaacgcg cacaatcgtg accacgccaa agcgcctgag aaatcgaaag cgggaattcg
    15901 cggcgttctc gatccggtcg gagatgcggc gcaggccctt gtggaagcgg cgtggaatga
    15961 gaatcttgtg ccaggtccac ttggtgtgaa ggttccaggg cgggcggaat tcgtggccgg
    16021 tcgcgtcgaa cgacgagaaa agccccgcgc acatcctccg cgcctcgccg atgcggtaaa
    16081 agcgcatgcg gatcgggtct ttatggcgaa agagatgaat gctcacgaat tgaaggaagg
    16141 cgaacagcag gttctccggc gcgaagcgtc ccggcaccca cacatgagca tgcccgccgg
    16201 gtttgagcac ccgatggatc tcgcgaaaca cggcccggcc gttttccttg gaaagatgct
    16261 gcagtaccag aaggctgtac acggtgtcga aggagttgtc ggcgaaggga aggcgggttc
    16321 cgtccccgcg cacgaaggac gcgttccgtt tgttcgtgtt gatatagccc ctggccgccg
    16381 cgagcatgtc ggatgagatg tccatcccca ccgcattcgc gccgagcgcg catacgttgt
    16441 tgagcatgcg cccccagccg cagccgaggt cgagcacgcg ttcgccgggc gtcacgggaa
    16501 agggcggcgg gatcgcggcg atgtcgccgt agtagtcggg caggatggcc atcacgccgg
    16561 tggtgatgaa ggcggggtcg cgcttcgccc cgtcgtccca gacgccgacg aactcgtcga
    16621 ggaaggcgct atcggggacg ttcatgatgc tcgtttcgca cctcgcaggc ggccagcggc
    16681 gaggcgctcc gttctcccgc aagcgcggtc tcggtgtcga tggactccat cgcgcgcaac
    16741 accacgcgat agtcgttcgc tgcgttccag aagagatagc cgtaggcccc ggaatccttc
    16801 acggccttca cctgtttgat gatgtagtgt tcgttgtaat tgctcactcg ccagcgaaag
    16861 gcctgcagcc agggccggac ggttttcccc ttcgccagcg cgatcacctt tttgcatcct
    16921 tcgtggatga agtagtaggg atggtcgccg ggccgcgcgt agccgtcgaa atcgtcgttg
    16981 aagtgcgacg ggtagagcat gggcgagatg atgtcgcagt gctcggcgag acgctcgatc
    17041 cgctggccgg tcttgatgat gtcgatctcc ttgccccagg cgaccacgcc gaagatgtcg
    17101 atcgacagca ggacatttcg cttcgcgatc tcctcgtgcg cgcgcttcag gaaggcggtg
    17161 atggtgtcgt cgcgcgacat ggtcccgaaa tcataggcga actcggcgtc gccctgcttt
    17221 cccgtggtgg ggaagcggat atagtcgaac tgtatctcgt ccacgccctt ctcggccagc
    17281 tcgacggcga gcgcgagatt gtagtcctgg gccgccttgt tggtggggtc gagccatttc
    17341 tccttggcct cggcgcgcca gggcccgtcg cccgatttcg actggatggc ccagtcggga
    17401 ttgtgcttga acatgtgctg gtcgtggaaa agcgccaggc gggcgatcac atagatcttc
    17461 ttttccttta agatgcggat cagctggtcg atgtcgtcga tggtgcgctt gccgtgcgcg
    17521 tcggtctcga tcgcctccgg gacccggctc ttgtagttga cgatgccgca gacgtccttg
    17581 gcgtcgaaga cgatgcagtt gatgcccagc tccttgtact cgtcgatgtt ctcgagcagg
    17641 ctctcgcgcc cggcggagct ccccgtgtaa tacaggccgc gcgcgaaaat gagcggcggc
    17701 tttcgggaat tccgcatgtc gggcatgagc gcgggaagcg aatgagggat aaacaccatc
    17761 tggcccttct cgatctcgtc gccggagatg gcgttaaaac tcctaatggc gtcccggagg
    17821 cgataggcct tataaaagga agtgaagggc accgccaggt ccgctaccgc gcggaccgag
    17881 gtgtcggcgg gtgcggtata gaagagcata cgcccgcagt gcgtgaatcc cgccggcagc
    17941 gtacggtaaa tggcgtcata tcgccgggca gcgccggcat cggtcggggc cgtcgcgcga
    18001 ggtttttccg aattgccaaa atagcgcgct acgccgaaat taattgttga ataaataacc
    18061 gcggcgccta cgataccgat cgcgatggca atgatgcgtc gccgggtaaa gatgatggga
    18121 ttgaacatgt ttttcatagt agtgccggtt ccgtgtgcgc ccattgggcg gcggcgcggt
    18181 ttttaatcaa ccaaaaatag catgagcggg acaataatca tcatccgggg gctgcttggt
    18241 aaatgtttcg gcagcttcga gggcgttcgg aagttttttg ccttcgtgcg ctccattttt
    18301 gtgtcattcc gttcgctgcg ctatctgcgc ctgcgctcgc tggtggccat cgcggtaaac
    18361 cagacgcgct tcaccggcta cgacgcgctt cccatcatcg tggtcatctc gctcatcctg
    18421 ggggcgacgg tcatcatcca ggcgacgcag aacttcccga agttcgggat cgagggcttc
    18481 atcgggaacc tgctggtcat catcatcgcg cgggagctgg ggccgttggc cacggccatg
    18541 gtggtgatga gccgctcggg ctcggccatc gcgaccgaga tcgccacgca gaagcagggc
    18601 cgcgagatac agtcgctcga gctcatgggg atcgactcgc ggctgtatat cgtgatcccg
    18661 cggatcgccg cctccatcgc gtcgatcctg tcgctcatca tcatcttcga catcgtcgcc
    18721 tttttcggcg gctatctcat ctcgctgtcg accgtcttca tacccatcga catcttcctg
    18781 cagacgctgc tggactccat gagcgcggac gatctcatca tcaccctggt gaagagcgtc
    18841 gtttacggga ccacgatccc gctcgtgtgc tgttattacg gcttcatgcc gcggtccaag
    18901 ttcgagatcc ccatctatgt ttcacgcgcg gtgatgcgca cgctgctgat cctgttcgtc
    18961 gtgaacgcgc tgatctcggc gctgttctat ctgtgagcca tggaaacgac cgatatcatc
    19021 ctccgcctcg agaacgtggt ctacgacgcg gacgaattcg ccccgctcac cgggatcacc
    19081 ttcgagctcg cctggggcga ggacctggtg gtcttcggcc ccgaggactc ggggctcaac
    19141 accctgtgtc cgctgatcgc ggggctggcg ctcccggccg cgggcgagat ctacttcaag
    19201 agcgcaccga tcaaggggtt cgattatttc cagatgcacc gctaccgcaa ggagctcggg
    19261 t
//
LOCUS       DAORHF010000042        19054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2021_length_19054_cov_6.164324, whole genome shotgun sequence.
ACCESSION   DAORHF010000042 DAORHF010000000
VERSION     DAORHF010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 19054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19054
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2021_length_19054_cov_6.164324"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..1057)
                     /locus_tag="PKJ16_05655"
     CDS             complement(206..1057)
                     /locus_tag="PKJ16_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV46507.1"
                     /translation="MIVNHNMSAINSNRVLKFKHWDTNKSMEKLSSGMRINRAGDDAS
                     GLAVSEKMRTQIQGLRQAERNTEDGMSMVQTAEGYLDQTSSILQRIRVLAVQSSNGIY
                     TQLDRQLIQVEVSALIDEVDRIASQAEFNKFKLLLGEFSRTNPKASMWFHMGANMHQR
                     ERIYIGTMTSRGLNLKEPTGKFLANLSTADSANRTIGIIDDALHKVSKQRADLGAYFN
                     RLEFASKGLMNAYENIQASESRIRDADMAETLVDLTKDQILVQSGTAMLAQANMKSRT
                     ILQLLGT"
     gene            1590..3950
                     /locus_tag="PKJ16_05660"
     CDS             1590..3950
                     /locus_tag="PKJ16_05660"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46508.1"
                     /translation="MKMKITSKMLTGIRGRLLRLILLTSIIPLLAASLLIYTVARSSI
                     LNVTNTAMANAVHAIKQRSELQSAELDSRAQREIEGAARMARAVFAPFAVPELSRATQ
                     KLTVTNQDSGEESIIELPTMSSGGTVLNYNYGIVDTIAARIGVEGATATLFQLHDEKL
                     VRIATNVKTKEGKRAVLTYIPKESLVYRTVTAGKTYTGRAIVVGKWNITNYEPIKNRA
                     GAVVGVLYVGVPAPQTAIFDMVRGTKVSSHGYVYIINSNGQFIEHPRLKGKNILALTD
                     LGSGTHFGTRIIEQKEGGISYKFQTTVDGQLLQQHAVAYFTYFKPWDWIIVATADYDD
                     IFGGLNRLFAVMIALLVGFVVALFFVSNFFAAKITRPFRIIIDTAKKVANGDLTAFIR
                     QPQYLKCYETKNCQRTNCPGYGVRNRACWRIDGTLCGDGSVAMPSGDDKLLKHCYTCQ
                     VYRHAIKDEIDELIESLNNMIVTFRTVVGEIQDLTGELSGKADQLAEIGLNMGSATQN
                     QAAVIEETSSANEELMASIENVATAADTQAQRVSQTGAAMEELMSTIRVVSDNSTNVS
                     RETKATVEEAKKTGEMLQSTTRSIQQISESSKKITDITAMINDISDQINLLSLNAAIE
                     AARAGDHGKGFAVVAEEISKLADATASSSKEIHSLIGTSRSDIETGASLVMQMASAIT
                     AMIQKIEHAAKLVEEIALSSDEQIRGSEQVMHDVEDVNNMATQIANATNEQKSTSAEI
                     LNAVSKINEMVQEIASNAQFLADFSVSIKERSWKLRDTSARFTVKK"
     gene            4062..5075
                     /locus_tag="PKJ16_05665"
     CDS             4062..5075
                     /locus_tag="PKJ16_05665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV46509.1"
                     /translation="MGLNPFKESNLDDLQRSLKRFYDDRFGDTSKTLRGWWSRFVKKG
                     REKVTLMFIPHSEKQIVNFHISIFSIAGFFGAIALTVVVTSIIIVNHTSNTKEVSRLK
                     LYGASSKEHLVKYREEINRLHEIFQKFKPEVTYLYSIIPENDVDSLWAKGGPAQPETK
                     NPDAGDHAPPIELLNIEEIDRDLKITKDVLVKIKGFLEARKKILEYTPSIWPVNGYII
                     SAFGERTSPYNFQKEFHHGIDIAGYPGAEIHATAPGRVEDIKWDPVLGLTVSVKHKYG
                     FTTHYSHCQRVSVEPRQKISKGEVIGYVGRTGKAMRHLCYYQVQIGTEFVDPVPYLNK
                     LPE"
     gene            5141..6172
                     /locus_tag="PKJ16_05670"
     CDS             5141..6172
                     /locus_tag="PKJ16_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46510.1"
                     /translation="MNNGEHRFSFHATFTLLGGLLSGLVGFLYLLAIPITILSALPEK
                     WFAFAIITLALGPIFAFGLYVYRRHTGVIELTDDALTIVRGSSRRSIRLAEITAVRER
                     DRHVPPNMTFLAGSRSFRFSRKVEGFPYLYELLRARVPVMAANGPASLPLILDARRWY
                     RMNLYGAVVLICALWLILGVFSFSTRQFDNPIEVASILAACAALMSLMAFFGFKPGKQ
                     QPVMIAFDTNEISLAFFSGKRRTVPLDAVREIRIYRTSRDIATDYLAARNIEYPLGIT
                     ITDGTRYLVDEMCAKACRTTVERVFFALRDAYAGRGPVIENYRPSGGENPMRRVTSES
                     KRNGHTHDS"
     gene            6162..8258
                     /locus_tag="PKJ16_05675"
     CDS             6162..8258
                     /locus_tag="PKJ16_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46511.1"
                     /translation="MIPEPKETPLTIFPLEHERKRSAARAQPTELAGCLKLAPENRDR
                     LRMIAVKNPTMKARIDYLTAAARRPIGERLRAIIAGAVSLGWRVEKDYFLSYHKEEPA
                     ASGRLAPAGPEYTVKRVLRKNRFVYEIYVGESMRAPDRVIKSEQKVHKLGYPDDAVAL
                     LRELIKKLPRTRTTDVYKQVGDSIIQRVSVFRERFWHRSYYAVIFSTYYKGSSRDSRR
                     FFEKGYVPIDTLLPPDGQSPRHIIIDAGPNPEFIPRPTRGGSAAGTRFVIIPGEINFK
                     AHGDTLLPYAASSIASTQFLAAHPRFERIARHSGIADELIKAETLFVSERIVLSPRAI
                     ADAYLAVLRAYRTLRMTAAGTLLFSFFYGTLEGAIVNPMMAMIEHRYLVLGWVTVIAL
                     YFYPVIESLIEIKSGIKFDEVRNAEKSRRLPRVYINEELRAVQLRVALFYAAAAGVLM
                     LLYPAVFLRLIGGLPFAVPALFALFFCNKLFKDWAEYFEINNTFRIFIRTVGRDPVHH
                     RNLLKVRSFEEGLRMLLRVTGLGCGMALMWANPTVAIAVAGAGALIGCASKFVFPLFG
                     AEYAVSVSTDTRPFPLDNHNYALIPERVRLRAKRDPRFEARGPRWVSLDPQDIEILVD
                     GGDPDDVPSVRAYPGNTFSSLNKKWKLTWRHRCTIVIELHGSTPLELIRHQSGQQIRY
                     TLMRAQPSVEPNRGTP"
     gene            8255..9313
                     /locus_tag="PKJ16_05680"
     CDS             8255..9313
                     /locus_tag="PKJ16_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46512.1"
                     /translation="MTIRLSFIIITLASLTAAAPPLSTASLIDAIRDGSVGRVEKLLD
                     AGVGVNAPDAEGASPLHHAALSGAVPVINLLIARGANLNARDRTGMTVLMYAAMAGKV
                     EAVQRIVESNVDLNLMDRNGFSALMWAQRKKRAEVVTILTKAGAFDYLEAERREKELI
                     DASVAGNVVAVKMLLDAGVNINARGTKGSATPLIIAAERNQVDLLKLLLERGADINAR
                     DEKWQSALFHATANDRPDVIGILIQKNADVNARDKTGRTPLMMACHDAREQIMDMLLS
                     RGADVKARDMNGNTALMHLISGSGSRPFKLRAAEQLLRKKASPSAQADNGSTPLSLSK
                     KDGFTEMAELLVKAGAKK"
     gene            9419..9910
                     /locus_tag="PKJ16_05685"
     CDS             9419..9910
                     /locus_tag="PKJ16_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV46513.1"
                     /translation="MRNIAIIATLLVFIVACSAKRKENPPDPAALTVQEINAELAKVS
                     VVTFEYKSVALEKKEIAKWGEGVYPIIRDAIAKLPKGYVLQITGHADASGPEDVTSDK
                     RGNVYWSTERAMAAWQYLRGKGITSPQMTFKGVGSTDLIEGVDARDRRQRRITFKIIK
                     EQQ"
     gene            complement(9952..10779)
                     /locus_tag="PKJ16_05690"
     CDS             complement(9952..10779)
                     /locus_tag="PKJ16_05690"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HNV46514.1"
                     /translation="MNKMPQDTKKVFMKKGSCSQALCFLMNRDFGCHTDAGEKAADPF
                     AGGLLMRGHQCGMLWGSSLAVGGECHRRFPDSGRATAVAIATTGRLVDSYAQRAGSVN
                     CRDVCGHDFTKKTHLAIFMIKFILHINRYCLDLAEQWFPEAVRAAAEGLSTAPTYSLP
                     PMSCASVVAKRMGASDEEAVMVAGFAGGLGLSGNGCGALAAAVWMKTLGWSKQNPGKS
                     AYNNPAAKSTLQAFMDANGSRFICRELCGRTFASVDDHAKFLQAGGCRDVIESLART"
     gene            complement(10857..12461)
                     /locus_tag="PKJ16_05695"
     CDS             complement(10857..12461)
                     /locus_tag="PKJ16_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46515.1"
                     /translation="MKRYVGLMTLASLVLTVQVARAGFFGEDLSDYTRYVPFAQGESL
                     IVFSMQGKPWITLADEIEMKQLVLGAIALPGLPADKGGELDAMKREFARMYDSARAVG
                     MDFFRETAVFVHTGKKEQLNPYDAFARYIVVVRVNMKWKPTGDDAVMKQAAELFKTIQ
                     NIPETSPVPLLGSFAVRFGDFIVLGDNQSIADYLARRKEKRTNDDPAVKRVNAAIGPA
                     AFFHWSTKNPHGLTPMMEGMMKNNTRRAQQYHEMRKLHLDLMNKFAEPGAAYTIEDVD
                     HIQKCINHVEERVDISGGLRVTVVVRGSINRYVPLLRRMATSPDYLRLVMGPMNASLG
                     TDLTPEEVEYSADVDSDYTDFTMTGRMGIKREVVKRLMARQVQNAIAAFVMGHSQSSR
                     MLVRPKKPDGAAHDNKTAETDRTPADPGAGFIESGWVDDDNYRVLAAGSPKAGLTNAV
                     QRRLSARSNALAAAHKKALHEFRVMRFRNPNLQARASEAAIAKEFGGLARAGVIVRER
                     YQDDGGCDLVLQVSAPGLRRKVTEGK"
     gene            complement(12466..13344)
                     /locus_tag="PKJ16_05700"
     CDS             complement(12466..13344)
                     /locus_tag="PKJ16_05700"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV46516.1"
                     /translation="MKGYPLLVAAIALCAVVSSCTRDSGVERMKAAIDAGNLAGVEKC
                     VADGLEVNANLGGGATPLHLAALRGSAPIAAFLIGKGAKVDAADEMGFAPLHHAAATG
                     SADVVKLLLAKGANVKLVNKLGENALSMLAGGSGEKFLAIARILVDARIPLDPQSAQG
                     GATPLIVAATHGNMDLVKLLLVKGADPSIKNAEGKTALALLRGVNNPGVLAQFQRLLD
                     ARLIEAILNGDAPRVKSSLAAGANPNAKTGIMSEWASQTNLSNIPGRNLSVLQFAEEQ
                     KNPEIIDMLQAAGAKE"
     gene            13493..13954
                     /locus_tag="PKJ16_05705"
     CDS             13493..13954
                     /locus_tag="PKJ16_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46517.1"
                     /translation="MAIRIIPITMLMMIATTVACTKISGTAAATGPALGDWSAPIDTC
                     STADRERLVGAYLRSTKNPNIAVKIVKDAFRGVYLAVSIPSTCQPDGSCQAAVVTREM
                     CSKFQADVFFTSVVSSDDYRAADGHLFAECVVPLGARKSAFTASVRFVNCQ"
     gene            complement(14034..14666)
                     /locus_tag="PKJ16_05710"
     CDS             complement(14034..14666)
                     /locus_tag="PKJ16_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46518.1"
                     /translation="MKHEQLVFEALSYYRYRGIDANTLRGFLGVHEDRKHTITNDRRA
                     LDRFEREGLLYRVGRKWFLTGPGLARSRGRAYEPEWQPEDSWVLLALLYADDSRPCPL
                     VDLISIADYMNHSVLTREEAHGAINRLVAGKLISVRAGAFVPSAAARAMMKKIEATCG
                     KYVHGHLQGLARMLVCPCCGVKLKRVTWRYRIDEADMKRAVADYLRRMKG"
     gene            complement(14760..15626)
                     /locus_tag="PKJ16_05715"
     CDS             complement(14760..15626)
                     /locus_tag="PKJ16_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46519.1"
                     /translation="MKSRIAGLLACALLVVCGAVAGADSFHDSPVREVVAEVWPGWLD
                     EETIQLRVTVSLARHDRVGPYGGALINAVQFAQRASAMLYADTVRGVYAGRPPKRSQL
                     AFADGLDFFIKGGMVIDAKFEGPYRCVIVYRQPARGIVILPGMGAAIAKDAHRSAAKP
                     RFPVDGAIDYRNPPKNLATEWWDDDNTLRIRAWGAPRYSSTSRADRRYHAANAAELAA
                     QARVLAIVRGITVRGGRFADPDSRSGTIVEKRFDENDTCEILYEVKRKGLRKRIEADL
                     VGRPADAKPGQM"
     gene            15829..17163
                     /locus_tag="PKJ16_05720"
     CDS             15829..17163
                     /locus_tag="PKJ16_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46520.1"
                     /translation="MNNGVFLLVFFGIMAALIVVLTRWENRKRESLSGTARRLLNELA
                     TRLQLTGNIAIRPDALEITDSIDDVSITIVADLDPTRKSIAERDLSATLTLKGSFRIS
                     GVAEIRTEVYPHTIGNIVDEDHDIVTGDREFDRRIFLEGKSSDAARALMDKPLRDDLV
                     RLCATGASFYLRFGSFTARIGIASVRDRDALAEIAQLMRTIGARLAEEIDVPARLLHN
                     ATTDPVPEVRLRNLRALTTHFPDSDTITAALERLLGDESVLIQIESARALGDRGVPHL
                     VRILRTAGTLDEKTVVTMVAHFRRLRSSESVPALAHIYEHYRATAILSEVLHAFAEFG
                     DERVSALLAGQLDRFDGELHALLIEALGTCGTVDVVERLYAIGKNSLNPFLRNAVQKS
                     IAKIQARLGNVDAGWLSLPDADGAVGGLSVADGAKDGGLSVSGDSTQSGKYK"
     assembly_gap    17288..17387
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17509..>19054
                     /locus_tag="PKJ16_05725"
     CDS             17509..>19054
                     /locus_tag="PKJ16_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV46521.1"
                     /translation="MKDTHLAIDLGTTNTVVARWNDEFDGPEILHIDSICRSANSTKE
                     IDDSYTIPSCVYLRHPSEVLSFPWKYFFANFRSKTGAYIGRQALEKDGGVYRPCFVNH
                     FKMHLGKNSFQFIGRLGRWKYTAEDVVRIFLKELFETVRQSINRDPEYVTFCAPVDFY
                     EFYRARLQRICTGLGVESVKTIDEPVAAALGYGLSIDEPRSALVIDFGAGTLNIALIA
                     IGEAGRDTGHCEVIAKDSISVGGTTVDSWIVEEICEYYGYDFARLSSDEAMRWWYRML
                     LGEACRIKESLFLKTRDTFFLMPSHLMAQYARAIPGERVDQKRPVDYTRDELVALLEK
                     RGLYALLNNLIDSITQEAKRKGIGEKSIDDVLMVGGSTLLPRIYATVEKRFGRDRVRA
                     WQPFNAVAFGAAAYAAGRFNKADHITHDYALMTYDRETHEPEYTVVVPRGTPYPTPRD
                     FWRRQLIPTCALGEPERIFKLIICEIGRRHSANPEFVWDEKGALHLMKAGDDRSLVIP
                     LNESDPT"
BASE COUNT         3676 a         6160 c         5602 g         3516 t
ORIGIN      
        1 taacggacgc gaccgcgata attccgaaag aacccacgac ccgcctttat gacgccgttg
       61 aatatatcat ggcgtactgc attttttctg ggttaacatc aacccgctaa tgcgacataa
      121 tctaaaccgc ggacagaaag agacccgccg ttcccgcggc gggtctcgtg gttgttcggt
      181 attccctctg tacgactcgt gttctttatg tccctaagag ctgcaagatc gttcttgact
      241 tcatgttcgc ttgggccagc atcgcagtac cactttgtac aagaatctga tccttcgtga
      301 ggtctacaag ggtttcggcc atgtcggcat cccggatccg gctttcggaa gcctggatgt
      361 tttcataggc gttcatgaga cccttcgagg cgaactcgag gcggttgaag taagcgccaa
      421 ggtccgcacg ctgtttcgac actttatgga gcgcgtcgtc gatgatcccg atcgtcctgt
      481 tcgcgctatc ggcggtggac aggttggcca ggaacttgcc ggtgggctcc ttcaaattga
      541 gtccgcgcga ggtcatggtg ccgatataga tgcgttcacg ctggtgcatg ttcgcgccca
      601 tgtggaacca catgctcgcc ttgggattgg tccgggagaa ctcgcccagg aggagcttaa
      661 acttgttaaa ctcggcctga gaggcgatcc ggtcgacttc gtcgataagc gccgaaacct
      721 cgacctggat cagctgacgg tccagctgcg tgtaaatacc gttcgacgat tgaacggcga
      781 gcaccctgat ccgctgaagg atcgacgagg tctgatcgag atacccctcg gcggtctgga
      841 ccatcgacat gccgtcttca gtgttccgct cggcttgccg gaggccctgg atctgggtcc
      901 gcatcttttc ggaaacggcc agcccggacg cgtcgtcgcc ggcgcggttg atccgcatac
      961 cggaggacag tttctccatg gacttgttcg tgtcccaatg cttgaacttc aggacccgat
     1021 ttgagttgat ggcactcatg ttgtgattca ctatcatacg aatacactcc tttgtcttgt
     1081 aatagcctga tctccgtatc aggcctgtaa taccaggttg cgttctgtta ttcagggaat
     1141 acccaaataa ttactgcaac cattccgccc tgctcctatt caggttgaaa tgcttgccgc
     1201 aattcccatc agttttcaaa gaccgccccg gcctgccctg aaccggtttt cgtcccttac
     1261 gaataaatga tcggattcac atgaggacaa attgaagccg atttggcctt atttatggcc
     1321 gtttatgatg tttttatcga attattggca aatacggaac tgttttgtct atttattgat
     1381 ttttttgcgt atttttgctt attgtttatt tcggcattct gatttttacc atgagccttt
     1441 ttccaaaaaa tatttgaaat gtaatccgct tccatgctaa tcatcttcaa tgaaattgtg
     1501 aggataaata tttatttgac atattttgtt tattaatatt tttacacatc ggcgcacacg
     1561 ccccggcgac caatccagga gtggcatcca tgaagatgaa aataaccagt aaaatgctca
     1621 cggggatccg cggtcggctg ctgcggctca ttctactcac ctccatcatc ccgctcctgg
     1681 cggcgtcgct cctcatatac accgtcgcgc gcagctccat cctgaatgtg accaacaccg
     1741 cgatggccaa cgccgtccac gccatcaaac agcgcagcga actccaaagc gcggagctcg
     1801 acagccgcgc ccagcgggaa atcgaaggcg ccgcgcgaat ggcgcgtgcg gtgttcgccc
     1861 ccttcgccgt cccggagctg tctcgggcca cgcaaaagct caccgtgacc aaccaggact
     1921 cgggcgagga gagcatcatc gagttgccaa ccatgagctc cggcgggacc gtcctgaact
     1981 acaattacgg catcgtggac acgatcgcgg cgcgcatcgg cgtcgagggg gcgaccgcga
     2041 ccctgttcca gctccatgac gaaaagcttg tccgcatcgc caccaacgtg aaaacgaaag
     2101 agggaaaacg cgcggtcctc acctatattc ccaaggaatc gctggtgtac cggacggtga
     2161 ccgcgggaaa gacctatacg ggccgtgcga tcgtggttgg gaaatggaac atcaccaatt
     2221 acgagcccat caagaaccgc gccggcgccg tggtgggcgt tctctacgtg ggcgtacccg
     2281 ccccgcagac tgcgatcttc gacatggtac gaggtacgaa ggtctccagt cacggatatg
     2341 tgtacatcat caactccaac ggccagttca ttgaacaccc gcggctaaag gggaaaaaca
     2401 tcctcgcgct caccgacctc ggctcgggca cgcacttcgg gacccggatc atcgaacaaa
     2461 aggagggcgg gatctcctac aagttccaaa ccaccgtgga cggccaattg ctccagcagc
     2521 acgccgtcgc gtatttcacg tatttcaagc cctgggactg gatcatcgtg gccacggccg
     2581 actacgacga catcttcggc ggcctcaacc ggttgttcgc ggtcatgatt gcgctcctcg
     2641 tcgggtttgt cgtcgcgctc tttttcgtga gcaacttctt tgcggccaag atcacgcgcc
     2701 cgttccgcat catcatcgac acggcgaaga aggtggccaa tggcgacctg accgccttca
     2761 tccggcagcc gcaatacctg aaatgctacg agacgaaaaa ctgccagcgc acgaactgcc
     2821 cgggctacgg cgtccgcaac cgcgcctgct ggcgcatcga cgggacgctg tgcggcgacg
     2881 ggtcggtcgc catgccaagc ggcgacgaca agctgctcaa gcattgttac acctgccagg
     2941 tctaccggca cgccatcaag gacgagatcg acgagctcat cgaatcgctc aacaacatga
     3001 tcgtgacctt ccggacggtg gtgggcgaga tccaagacct caccggcgag ttgagcggaa
     3061 aggccgacca gctcgccgag atcggcctga acatgggcag cgccacgcag aaccaggcgg
     3121 cggtgatcga ggagacctcc tcggccaacg aggagctcat ggccagcatc gagaacgtgg
     3181 ccaccgcggc cgatacccag gcgcagcgcg tgtcgcagac cggcgcggcg atggaggagc
     3241 tcatgtccac catccgcgta gtgagcgaca actcgacgaa cgtgagccgc gagaccaagg
     3301 ccaccgtgga ggaggccaag aagaccgggg agatgctgca gtccaccacc cgcagcatcc
     3361 agcagatatc ggagagctcc aagaagatca cggatatcac cgcgatgatc aacgacatct
     3421 ccgaccagat caacctgctg tcgctgaacg ccgcgatcga ggccgcgcgc gcgggcgacc
     3481 acggcaaggg gttcgcggtc gtggccgagg agatctcgaa gctcgccgac gcgacggcct
     3541 ccagctccaa ggagatccac agcctcatcg gcaccagccg cagcgacatc gagaccgggg
     3601 cctcgctggt gatgcagatg gccagcgcca tcaccgccat gatccagaag atcgagcacg
     3661 cggccaagct cgtcgaggag atcgcgctct cctccgacga gcagatccgg ggcagcgagc
     3721 aggtgatgca cgacgtggag gacgtgaaca acatggccac ccagatcgcc aacgccacca
     3781 acgagcagaa atcgaccagc gccgagatcc tcaacgccgt ctcgaagata aacgagatgg
     3841 tccaggagat cgcgtcgaac gcgcagtttt tggcggattt ctcggtgtcc atcaaggagc
     3901 gctcctggaa gttgcgggac accagcgccc gcttcacggt aaaaaaataa tcggcttgac
     3961 aggcaaaaaa acggccgtag agttccgtcc aatcacggcc ggaacggcgg gggtgatggg
     4021 cgcccggacg ggcgcccccc gcgaacgtaa tcagagggaa catgggtctc aatccgttta
     4081 aagaatcgaa cctggacgat ctgcagcgca gtctcaagcg cttctacgac gaccgcttcg
     4141 gcgacacgag caagacgctg cgcggatggt ggtcccgttt cgtcaagaag gggcgggaga
     4201 aggtcaccct catgttcatc ccccactccg agaagcagat cgtgaacttc catatttcga
     4261 tcttctcgat cgcgggcttc ttcggcgcga tcgcgctcac ggtcgtcgtc acctccatca
     4321 tcatcgtgaa ccacacctcc aacaccaagg aggtgtcgcg cctcaagctc tacggggcca
     4381 gctcgaagga gcacctggtg aagtaccgcg aggagatcaa ccgcctccac gagatattcc
     4441 agaagttcaa gccggaggtg acctacctct actcgatcat tcccgaaaac gacgtcgatt
     4501 ccctctgggc caagggcggt cccgcgcaac cggaaacgaa aaacccggac gccggcgacc
     4561 acgccccgcc catcgagctt ttaaacatcg aggagataga ccgcgacctc aagatcacca
     4621 aggacgtgct ggtaaagatc aagggcttcc tcgaggcgcg caagaagatc ctggagtaca
     4681 ccccgtcgat ctggccggtg aacggctaca tcatctccgc gttcggcgag cgcacctcgc
     4741 cctacaactt ccagaaagag ttccatcacg ggatcgacat cgcgggctac ccgggggccg
     4801 agatccacgc caccgcgccg gggcgcgtcg aggacatcaa gtgggacccg gtgctgggac
     4861 tcaccgtgtc cgtgaagcac aagtacggtt tcaccacgca ctactcgcac tgccagcgcg
     4921 tgtccgtcga gccgcgccag aagatatcca agggcgaggt gatcggctac gtggggcgca
     4981 ccggcaaggc gatgcggcac ctctgctact accaggtgca gatcggcacg gagttcgtgg
     5041 acccggtccc atacctgaac aagctgccgg aatagcggac gcctcgcggc ggtttccgca
     5101 gcgccgtctg tatcgccccc cgggttctgg cggtcatgag atgaacaacg gcgaacatcg
     5161 gttttccttt cacgcaacgt tcaccctcct gggcggcctg ctgtcggggc tcgtcgggtt
     5221 tctctatctg ctcgccattc ccatcacgat cctgtccgcg cttccggaga aatggttcgc
     5281 gttcgccatc atcaccctcg cgcttgggcc gatttttgca ttcggactgt atgtctatcg
     5341 ccgccacacc ggcgtgatcg agctgaccga cgatgcgctg accatcgtgc gcggatcatc
     5401 gcgccgctcg attcgcctcg ccgagatcac cgccgtgcgc gaacgggacc gccacgtgcc
     5461 gccgaatatg acgttcctcg ccggctcgag gtcgttccgc ttcagccgca aggtggaggg
     5521 gttcccgtat ctctacgagc tcttgcgcgc gcgcgtgccc gtcatggcgg ccaacgggcc
     5581 cgcgtcgctc ccgctcatcc tggacgcccg ccggtggtac cggatgaacc tgtacggcgc
     5641 ggtcgtgctt atctgcgcgc tgtggctgat tctcggcgtg ttctcttttt cgacgcgcca
     5701 gttcgacaat ccgatcgagg tcgcttcaat tcttgccgcc tgcgccgcgc tgatgtcgct
     5761 catggccttt ttcggcttca agcccgggaa gcagcaaccg gtcatgatcg cgttcgacac
     5821 gaacgagatt tccctcgcgt tcttttcggg gaaacgcagg acggtcccgc tggacgcggt
     5881 gcgcgaaatc cgcatatacc gaaccagccg ggatatcgca acggactatc tcgcggcccg
     5941 gaacatcgaa tatccgctgg gcatcacaat aaccgacgga acgcgctacc tcgtcgatga
     6001 gatgtgtgcg aaggcctgtc gcacaacggt ggaacgcgtc tttttcgctt tgcgcgacgc
     6061 ctatgccggt cgcggtcccg ttattgagaa ctatcgcccg agcggcgggg agaaccccat
     6121 gcgccgggtc acatccgaat caaagcggaa cggccacacg catgattcct gaaccgaaag
     6181 agacgccgct cacgatcttc ccgctcgagc acgagcgaaa gcgatccgcc gcgcgggcgc
     6241 agccgacaga actggccggc tgcctcaagc tcgcgcccga gaaccgcgac cgcctgcgga
     6301 tgatcgccgt gaagaacccc accatgaagg cccggatcga ctatttgacc gccgccgcgc
     6361 gccgtccgat cggcgagcgt ctgcgcgcga tcatcgccgg cgccgtttcc ctgggctggc
     6421 gcgtcgagaa ggactacttc ctgagctacc acaaggagga gcccgcggcc tcggggcggc
     6481 tcgcgcccgc cgggccggag tacacggtca agcgcgtcct cagaaagaac cgcttcgtgt
     6541 acgagatcta cgtgggcgaa tcgatgcgcg cgcccgatcg cgtgatcaag agcgagcaga
     6601 aggtgcacaa gctcgggtac cccgacgatg cggtcgcgct cctacgcgag ttgatcaaga
     6661 aacttccccg cacccgcacg accgatgtgt ataaacaggt gggtgattcc atcatccagc
     6721 gcgtctccgt cttccgcgag cggttctggc accgaagcta ttacgccgtg atcttttcta
     6781 cgtactacaa gggctcctcg cgcgacagcc ggcgcttttt cgagaagggc tacgtcccca
     6841 tcgatacgct tctgccgccg gacgggcagt cgccgcgaca tatcatcatc gacgccggcc
     6901 ccaaccccga attcattccc cgcccgacgc ggggcggatc cgccgcgggg acgcggttcg
     6961 tcatcattcc gggggaaatc aacttcaaag cgcacgggga cactctgctc ccctacgccg
     7021 cttcctcaat cgcctcgacg caatttttgg cggcgcaccc gcgcttcgag cggatcgcgc
     7081 ggcattccgg catcgccgat gagctcatca aggccgaaac cctcttcgtg tccgagcgga
     7141 tcgtcctgtc cccgcgcgcc atcgcggacg cctatctcgc ggtgctgcgc gcgtaccgca
     7201 cgctccggat gaccgccgcc gggacgctcc tgttcagctt cttctacggg acgctggagg
     7261 gcgccatcgt caatcccatg atggcgatga tcgaacaccg ctacctggtg ctgggctggg
     7321 tcacggtgat cgccctgtac ttttacccgg tgatcgaatc gctcatcgag atcaagtcgg
     7381 ggatcaagtt cgacgaggtg cgcaatgccg agaagagccg ccgcttgccg cgggtgtaca
     7441 tcaacgagga gctgcgcgcc gtgcagctcc gggtcgcgct cttctatgcc gcggcggccg
     7501 gcgtcttgat gctgctgtac ccggcggtct tcctgcgact catcggcggg cttccgttcg
     7561 cggtcccggc gctcttcgcg ctctttttct gcaacaaact cttcaaggac tgggccgagt
     7621 acttcgagat caacaacacc tttcggatat tcatccggac cgttgggcgc gatccggtcc
     7681 atcatcgcaa cctgctcaag gtgcgctcct tcgaggaggg cctgcgcatg ctccttcgcg
     7741 tcacggggct cggctgcggc atggcgctca tgtgggccaa cccgaccgtc gcgatcgccg
     7801 tggcgggcgc gggagcgctc atcgggtgcg cgtcgaagtt cgtcttcccg ctcttcggcg
     7861 cggaatacgc ggtgtcggtc tcgacggaca cgcgcccctt tccgctggac aatcacaatt
     7921 acgcgctcat ccccgagcgg gtccgtctgc gggcgaagcg cgatccccgt ttcgaggcgc
     7981 gcggccctcg ttgggtctcg cttgacccgc aggacatcga gatcctcgtc gacggcgggg
     8041 atcccgacga cgtgccgtcg gtccgggcat acccgggcaa caccttcagt tcgctcaaca
     8101 agaaatggaa gctcacctgg cggcatcgct gcacaatcgt catcgagctc cacggcagca
     8161 ctcctctcga gctgatccgc caccagagcg ggcagcagat ccgatatacg ctgatgcgcg
     8221 cacaaccatc cgttgaaccg aaccggggga cgccatgacg atacgactgt cgttcatcat
     8281 catcacgctc gcgagcctca cggcggccgc cccgccgctc tcgaccgcgt cgctcatcga
     8341 cgcgatccgc gacggcagcg tggggcgcgt cgagaagctg ctcgacgccg gggtgggcgt
     8401 gaatgctccg gacgccgagg gcgcatcccc actgcaccac gcggccctga gcggcgccgt
     8461 ccccgtgata aatctcctca tcgcgcgcgg ggcgaacctc aacgcgcgcg accgcacggg
     8521 catgaccgtg ctgatgtacg cggccatggc ggggaaggtc gaggcggtgc agcgcatcgt
     8581 ggaaagcaac gtggacctga acctgatgga ccgcaacggc ttttcggccc tcatgtgggc
     8641 gcaacgcaag aagcgcgccg aggtcgtgac gatcctcacg aaagccgggg ccttcgacta
     8701 tcttgaggcg gaacgtcgcg agaaggagct catcgacgca tccgtcgcgg gcaacgtcgt
     8761 tgcggtgaaa atgctcctgg acgccggcgt caacatcaat gcgcgcggca cgaaggggag
     8821 cgcgacgccc ctgatcatcg ccgccgagcg gaaccaggtg gaccttttga agctcctgct
     8881 cgagcgcgga gccgatatca acgcccgcga cgaaaaatgg cagagcgccc tctttcatgc
     8941 gaccgcgaac gaccgcccgg acgtcatcgg gatcctgatc cagaaaaacg cagatgtgaa
     9001 cgcgcgcgac aagaccgggc gcaccccgct catgatggcc tgccatgacg cccgcgagca
     9061 gatcatggac atgctccttt cgcgcggcgc cgacgtcaag gcgcgcgaca tgaacgggaa
     9121 caccgcgctc atgcatctga tatccggatc gggcagccgt cccttcaagc tcagggcggc
     9181 ggagcaattg ctgcggaaaa aggccagccc ctcggcgcag gccgataacg ggtcgacccc
     9241 gctctctctg tcgaaaaagg acggtttcac agagatggcc gagctgctcg tgaaggcggg
     9301 agcgaagaaa taacaccccg cgcacattca cttgacaaat cccgaaacat ccgtattgga
     9361 taccgtcgtt tggtccacgt ccccagagag agatcacgtt tcaagcggag gaaattcgat
     9421 gaggaatatc gcgataatcg ccacactgct cgttttcatt gtcgcctgtt cggccaagag
     9481 aaaggagaac ccgcccgatc cggccgcgct caccgtgcag gagatcaacg ccgagctcgc
     9541 gaaggtgagc gtggtcacct tcgaatacaa gagcgtcgcg ctcgagaaaa aggagatcgc
     9601 gaagtgggga gagggcgttt acccgatcat cagggacgcg atcgcgaagt tgccaaaggg
     9661 ctatgttctg caaatcacgg gccacgccga cgcgagcggc cccgaggacg tgaccagcga
     9721 caagcggggg aacgtctact ggtccaccga gcgcgcgatg gcggcctggc agtatctccg
     9781 cggcaaggga atcacctcgc cgcaaatgac cttcaagggc gtgggatcga ccgatctcat
     9841 cgagggcgtg gacgcgcgcg accgccggca gcgccgcatc accttcaaga tcatcaagga
     9901 gcagcagtag ggggatcgcg ccttattgta tacgcactcg attgtaacac ttcacgttct
     9961 cgcaagcgac tcgatcacat cccgacaccc gcccgcctgc agaaacttcg cgtgatcgtc
    10021 taccgacgcg aacgtcctcc cgcacagttc ccggcagata aaacgcgaac cgttcgcgtc
    10081 catgaacgcc tgcagggtcg atttcgctgc gggattattg taggcggatt tcccgggatt
    10141 ttgtttgctc catccgaggg tcttcatcca caccgcggcg gccagcgcgc cgcaaccatt
    10201 gccgctcagg ccaagcccgc cggcgaatcc ggccaccatc accgcctcct cgtcggaagc
    10261 ccccatccgt ttcgcgacaa cggatgcgca actcatgggc gggagcgaat acgtcggcgc
    10321 ggtcgacagc ccctcggcgg cggcacggac cgcctccggg aaccattgct ccgcgagatc
    10381 caggcagtag cggttgatat ggaggatgaa cttgatcatg aagatcgcga ggtgcgtctt
    10441 cttggtgaag tcgtgcccgc agacgtcgcg gcagttcacg ctgcccgccc gctgcgcata
    10501 ggaatcgacg agccgtccgg tcgtcgcgat ggcgacggcg gtcgcgcggc cgctgtcggg
    10561 aaaccgtcga tggcactccc cgcccaccgc gagcgacgag ccccagagca tgccgcactg
    10621 gtgtccccgc atcagcagac ccccggcgaa cggatcggcg gccttctcgc cggcatccgt
    10681 gtggcagccg aaatcacggt tcatcaaaaa gcacagggcc tgcgagcagg agcccttttt
    10741 catgaacacc ttcttggtgt cctgcggcat tttgttcatc gctcccacca tgtggtacag
    10801 aacaagtata gaattaattc ttccgcatca gcggtcacct gatgcagacc gctgcgctac
    10861 ttcccttccg tcaccttccg ccgcagtccc ggcgcgctca cctgcagcac caggtcgcag
    10921 ccgccgtcgt cctgatagcg ctcgcgcacg atgacgccgg cgcgcgccag accgccaaac
    10981 tctttcgcga tcgccgcttc cgacgcacgg gcctgcaggt ttggattcct gaagcgcata
    11041 acgcggaatt cgtgcagcgc ctttttatgc gcggcggcaa gcgcgttcga gcgcgcggac
    11101 agccgccgct gaaccgcatt ggtcaggccc gccttcggcg aaccggccgc cagcacccgg
    11161 tagttgtcgt catcgaccca tccgctctcg atgaatccgg cgcccggatc agcgggcgta
    11221 cggtccgttt ccgccgtttt gttatcgtga gcggcgccgt cgggcttctt cggccgcacg
    11281 agcatccgcg agctctgcga atgtcccatg acgaatgccg cgatggcgtt ctgcacctgt
    11341 cgcgccatga gccgcttcac gacctcgcgc ttgatgccca tgcgtcccgt catggtgaaa
    11401 tcggtgtagt ccgaatcgac atcggccgaa tactcgacct cctccggcgt gaggtcggtc
    11461 cccagggagg cgttcatggg ccccatcacc aggcgaaggt agtccgggct cgtggccatc
    11521 cgccgcagca gcggcacgta gcggttgatc gagcccctga cgaccaccgt cacgcgcagc
    11581 ccccccgaga tatcgacgcg ctcctcgacg tgattgatgc acttctggat atgatcgacg
    11641 tcctcgatcg tgtaggccgc gcccggctcg gcgaatttgt tcatgaggtc gagatgcagc
    11701 ttccgcatct cgtggtattg ctgggcccgg cgcgtgttgt tcttcatcat cccctccatc
    11761 atcggcgtga ggccgtgggg attcttcgtg gaccaatgga aaaacgcggc ggggccgatg
    11821 gcggcgttca ccctcttcac ggcgggatcg tcattcgtcc gcttttcttt tcttcgcgcc
    11881 aggtagtcgg cgatcgactg attgtccccc agcacgatga aatcgccgaa gcgcacggcg
    11941 aaggagccca gcagcggcac ggggctggtc tcggggatgt tctgtatcgt cttaaaaagc
    12001 tccgccgcct gcttcatgac ggcgtcgtcg cccgtcggct tccatttcat attcacgcgc
    12061 acgacgacga tgtaacgcgc gaaggcatca tagggattga gctgctcctt cttcccggtg
    12121 tgcacgaaga cggccgtctc acggaagaaa tccatcccca cggcccgggc cgaatcatac
    12181 atgcgcgcga actcccgctt catcgcgtcg agttcgcccc ccttgtccgc cggaaggccc
    12241 ggcagcgcaa tcgcgcccag caccagttgt ttcatctcaa tctcgtcggc gagcgtgatc
    12301 cacggtttgc cctgcatcga gaagacgatc agcgattccc cctgcgcaaa cggcacgtag
    12361 cgcgtgtaat cggaaaggtc ctccccgaag aacccggcac gggcgacctg cacggtgagc
    12421 acgagcgagg cgagcgtcat caggccgaca tatcgtttca tcggattatt ccttcgcgcc
    12481 ggcggcctgc agcatgtcga tgatctcggg gttcttctgc tcctcggcga actgcagcac
    12541 cgagaggttc cgccccggga tattgctcag gttggtctgg ctcgcccatt cgctcatgat
    12601 gcccgtcttc gcgttgggat tggcgcccgc ggcaagggac gatttcacgc gcggcgcgtc
    12661 gccattcagg atcgcctcga tgagccgcgc gtcgagcagg cgctggaact gcgccaggac
    12721 gcccggattg ttgacgcccc tgagcagcgc gagcgcggtc ttcccctcgg cgttcttgat
    12781 cgacggatcg gcgcccttca ccaggagcag cttcacgagg tccatgttcc cgtgcgtcgc
    12841 ggcgacgatg agcggcgtcg cgccgccctg ggcgctctgc ggatcgagcg ggattctcgc
    12901 gtcgacgagg atgcgcgcaa tcgcgagaaa cttttctccg gagccgcccg ccagcatgga
    12961 gagggcgttc tcgccgagct tgttcacgag cttcacgttc gcgcccttgg caagcaacag
    13021 cttgaccaca tcggcgctcc cggttgcggc cgcgtggtgg agcggcgcaa accccatctc
    13081 gtcggcggca tcgaccttcg cgcctttgcc gatgaggaag gccgcgatgg gcgcgctccc
    13141 ccgcaatgcc gcgagatgga gcggcgtggc ccccccgccc aggttcgcgt tcacctcgag
    13201 cccatccgca acgcacttct cgacgccggc cagattcccg gcgtcaatcg ccgccttcat
    13261 gcgttcgacg ccggaatcac gcgtgcacga cgacaccacc gcgcagagcg cgatggccgc
    13321 gacaagtaat ggatatcctt tcatggcagg cccttccttt gtatgatcgc gtatgcttgt
    13381 gcgaagaaaa taaaatgtca agcgggaatc gccgaccgga aatacatcat tgacagatca
    13441 tgcgcggcat ggcagcgtac gtggcgcacc gacgcgcccg gggaggacgc caatggcaat
    13501 acgaatcatt cccatcacca tgttgatgat gatcgccacg accgtggcct gcacgaagat
    13561 cagcggaacc gccgccgcga cgggaccggc gctcggcgac tggtccgcgc ccatcgacac
    13621 ctgctcgacc gccgaccgcg agcggctggt gggcgcctat cttcgcagca cgaagaaccc
    13681 gaacatcgcc gtgaagatcg tcaaggacgc gttccgcggc gtgtacctcg cggtgtccat
    13741 cccctcgacc tgccaaccag acggctcctg ccaggccgcc gtcgtcacgc gcgagatgtg
    13801 ctcgaagttc caggccgacg tcttcttcac cagcgtcgtc tcgtccgacg actaccgcgc
    13861 ggccgacggc catctcttcg ccgagtgcgt cgtcccactg ggcgcgcgca agagcgcgtt
    13921 caccgcgagc gttcgtttcg tgaactgcca gtgaacgcca tcgcgaggta gcgagacgcc
    13981 gtaaccgaac cgctccttcc tcgcgtggcg caacactatt gtccggatca ccatcaccct
    14041 ttcattcgcc gcagatagtc cgcaacggcc cgtttcatgt ccgcctcgtc gatccggtag
    14101 cgccaagtga cgcgcttcaa tttgacgccg cagcacggac acacgagcat ccgcgcgagc
    14161 ccctgcagat gcccgtgcac gtactttccg cacgtcgctt cgatcttttt catcatcgct
    14221 cgtgcggccg cactcgggac aaacgccccc gctcgcacgg agatcagttt ccccgcgacg
    14281 agacggttga tcgcgccgtg cgcctcctcg cgggtgagga ccgagtggtt catataatcg
    14341 gcgatagaga tgagatcgac cagcgggcag gggcgcgaat cgtcggcata cagaagcgcc
    14401 agcaggaccc aggagtcctc cggctgccat tcgggctcat aggcgcgacc gcgcgaccgc
    14461 gcgagaccgg gccccgttag aaaccatttg cggccgaccc gatagagcag tccctcgcgc
    14521 tcgaaccggt cgagagcgcg ccgatcattt gtgatcgtgt gctttcgatc ctcgtgaacg
    14581 cccaagaacc cgcgtagagt gttcgcatcg atgccgcgat agcggtagta cgagagcgcc
    14641 tcgaagacga gctgttcgtg tttcataatt caggtctcgc tgcgaaaatt cgggttcaca
    14701 caggtgatag tcgcgatatg gttcgcgaac gcattacctc gccgacacag acaccgatgc
    14761 tacatttggc ccggctttgc gtcggcgggc ctcccgacga gatcggcctc gatgcgcttc
    14821 cgcaggccct ttcgcttcac ctcgtagagg atctcgcagg tgtcgttctc gtcgaaccgc
    14881 ttctcgacga tggtgccgct gcgcgaatcg ggatccgcga agcgcccgcc acgcacggtg
    14941 atcccccgca cgatcgccag cacccgcgcc tgggcggcga gctccgcggc attcgcggca
    15001 tgatagcgcc gatcggcgcg gctggtcgag gagtagcgcg gcgcgcccca cgcacggatg
    15061 cggagcgtat tgtcgtcatc ccaccactcg gtcgcgaggt ttttcggcgg attgcgatag
    15121 tcgatggcgc catcgacggg aaagcgcggt ttggcggcgg atcggtgcgc gtcttttgcg
    15181 atcgcggccc ccattcccgg caagataacg atgccgcgcg ccggctgccg atacacgatg
    15241 acgcagcggt agggcccctc gaacttcgcg tcgatcacca tgccgccttt gataaaaaag
    15301 tcgagcccgt ccgcgaatgc gagctgcgat cgtttcggag gccgtcccgc atacactccg
    15361 cgaacggtgt ccgcatagag catcgccgat gcgcgctggg cgaactgcac ggcgtttatc
    15421 agcgccccgc cgtacgggcc aacccgatca tgccgtgcca gggacaccgt caccctgagc
    15481 tgtatggtct cctcgtcgag ccagccgggc cagacctcgg cgacgacctc ccgcacgggg
    15541 ctgtcgtgga aactatccgc acccgcgacg gcaccacaga cgacaaggag cgcacacgca
    15601 agcagtccag ctattcgcga tttcatcttt aatccttcgg atttttttat atgggctgcg
    15661 acatcatcca cgaatcggca aacggtatac ggcgatatcg caccgcagca atacattttt
    15721 cagtggacgc gcgaaaaatc gcgcagtatg ctgggcgtgt atgacgcatg caccgacata
    15781 ccatgtcatc gtcattgcgg agcttgatcc acattcgggg agcggaccat gaacaacgga
    15841 gtgttcctct tggttttttt cggtataatg gcggcgttga tcgtcgttct cacgcgctgg
    15901 gaaaaccgga agcgcgagtc gctgtccggg acagcgcgca ggctcctcaa tgagctcgcc
    15961 acgcgcctcc agctcacggg gaacattgcg attagaccgg acgccttgga aatcaccgac
    16021 tctatcgacg acgtctcgat aaccatcgtc gcggacctcg atccgactcg aaagagcatc
    16081 gccgagcgcg acctgtctgc gacactcacc ctcaaggggt cgttccgtat ttccggcgtg
    16141 gcagaaatcc gcaccgaggt gtatccccac accatcggga acatcgtcga tgaggaccac
    16201 gacatcgtga ccggcgatcg cgagttcgac cggaggatat tcctggaggg aaaaagttcg
    16261 gacgccgccc gcgcgctcat ggacaaaccg ctgcgcgacg atctggtgcg cctctgcgca
    16321 acgggggcgt cattttacct gcgttttggc agcttcacgg ctcgcatcgg catcgcctcg
    16381 gtgcgggacc gcgacgcatt ggcggagatc gcgcagctca tgcggacgat cggcgcgcgc
    16441 ctcgcggagg agatcgacgt ccccgcgcgc ctgctgcaca atgcgacgac cgacccggtc
    16501 cccgaggtgc gcctccgcaa cctgcgggcg ctcacaacgc acttcccgga cagcgatacg
    16561 ataacggccg ccctcgagcg gctcctggga gacgaaagcg tcctcatcca gatcgagtcg
    16621 gcacgcgccc tgggggaccg cggggtcccc cacctcgtcc gaatactccg gacggccgga
    16681 acgctcgatg agaaaacagt ggtcaccatg gtcgcgcatt tccgacgcct ccgttcctca
    16741 gagagcgtac cggcgcttgc gcacatttac gaacactacc gcgcaaccgc catcctgtcc
    16801 gaggtcctgc acgccttcgc cgaattcggc gacgagcgcg tgagcgcgct tctcgcgggc
    16861 cagctggacc gcttcgacgg ggagctgcac gccctcctca tcgaggccct cggcacctgc
    16921 ggcaccgtcg atgtcgtgga gcggctctac gcgatcggga agaactcgct caatcccttc
    16981 ctgcgcaacg ccgtccagaa gtccatcgcg aagatccaag cgcgcctggg gaatgtggac
    17041 gccggctggc tctcgctgcc ggatgcggac ggcgcggtgg gcgggttgag tgttgccgat
    17101 ggcgcgaagg atgggggatt gagcgtatcc ggcgattcca cacagagcgg aaaatataaa
    17161 taaaagagct gtcctgtaat attttattga aatttttatt tctttccacg atttccgtgg
    17221 ggtcagagcc tcccgtgggg tcagagcctc ccgtggggtc agagcctccc gtggggtcag
    17281 agcctccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncgt ggggtcagag
    17401 ccttccgtgg ggtcagagcc tcagtttttg attgatttct ttcgacggcc cgcgttatag
    17461 tccgtcagaa acagcggcgc tatcacacga caacggaaac cggcacacat gaaagacacc
    17521 cacctcgcga tcgacctggg caccaccaac accgtggtgg cgcgctggaa tgatgaattc
    17581 gacggcccgg agatcctcca catcgactcc atctgccgca gcgcgaactc gaccaaggag
    17641 atcgacgatt cgtatacgat cccctcgtgc gtgtatctgc gccatccgag cgaggtactc
    17701 tcctttccgt ggaagtattt cttcgcgaat ttccgatcga agaccggcgc ctatatcggc
    17761 cgccaggccc tggagaagga cggaggggtc tatcgtccct gtttcgtgaa ccatttcaag
    17821 atgcacctgg ggaaaaacag cttccagttc atcgggcggc tgggacgctg gaagtacacc
    17881 gcagaggacg ttgtgcgaat cttcctcaag gagctcttcg agacggtgcg ccaatccatc
    17941 aaccgcgatc ccgaatacgt taccttctgt gcgccggtgg acttctacga attctaccgg
    18001 gcgcgcctcc agcggatctg cacggggctg ggcgttgaga gcgtgaagac gatcgacgag
    18061 ccggtcgcgg cggcgctggg ctacgggctg tccatcgacg agccgcgcag cgcgctggtg
    18121 atcgacttcg gcgcggggac gctcaacatc gcgctcatcg cgatcggcga ggcgggccgc
    18181 gacacgggac actgcgaggt gatcgccaag gacagcatct cggtgggcgg caccaccgtc
    18241 gattcctgga tcgtcgaaga gatctgcgag tattacggct acgacttcgc ccggctctcc
    18301 agcgacgagg cgatgcgttg gtggtaccgc atgctgctag gcgaggcctg ccgcatcaag
    18361 gaatcgctct tcctgaaaac ccgcgacacc tttttcctca tgccctcgca cctcatggcg
    18421 cagtatgcgc gcgccatccc cggagagcgc gtcgaccaaa agcgtccggt cgattacacc
    18481 cgcgacgagc tcgtcgccct tctcgaaaaa cgcggcctgt acgcgctcct gaacaatctc
    18541 atcgactcga tcacgcagga ggcgaagcgg aagggtatcg gcgagaaaag catagacgac
    18601 gtgctgatgg tgggcggctc cacgctcctg ccgcggatct acgcgaccgt cgagaagcgc
    18661 ttcgggcgcg accgcgtgcg cgcctggcag ccttttaacg ccgtcgcctt cggcgccgcc
    18721 gcctacgccg ccggtcggtt caacaaggcc gaccacatca cccacgacta cgccctcatg
    18781 acctacgacc gcgagaccca cgaaccggaa tacaccgtcg tggtgccgcg cgggacgccc
    18841 tatcccaccc cgcgcgattt ctggcgccgg cagctcattc ccacctgcgc gctaggggag
    18901 ccggagcgca tcttcaagct catcatctgc gagatcgggc ggcggcattc cgcgaacccc
    18961 gagttcgtct gggacgagaa gggggcgctg catctcatga aggccggcga cgaccgctcg
    19021 ctcgtcatcc cgctcaacga gagcgacccc accc
//
LOCUS       DAORHF010000043        18201 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2175_length_18201_cov_5.614185, whole genome shotgun sequence.
ACCESSION   DAORHF010000043 DAORHF010000000
VERSION     DAORHF010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18201
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_18201_cov_5.614185"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2674)
                     /locus_tag="PKJ16_05730"
     CDS             complement(<1..2674)
                     /locus_tag="PKJ16_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46522.1"
                     /translation="MKKYIRLRLKRITPPLGVRGINRLMMRFIDRRAIILPVAVIMLG
                     TLVFMSAFEIGKTALLPHLTLVQSHILSVVSLTLLVTVVSFVVMIKIQRLVFQNITVV
                     EKRLQTEETLRKSQDRYKTLIQNINEYIYSVSYIDGVAVTTYHSPKCLAITGYAPEEY
                     YADPDLWYTMIHPDDRARVLDFTARILAEPLPAIEHRIIHKDGTERWISNNSAVKRDD
                     AGNVLQVNGFIQDITERKAAERALQESERRYRLLAENIGDVIWTMDENLKRLYVSPSI
                     RQLRGISVEEAMSESLDKIIAPEHLPGMRKRLAALMPDIKRGADIKPMTVEMEVLHKN
                     GARIPVEMIVSVVYDEDRNFAYFLGVSRDITERRKAQRELQQYREHLEDLVRERTREL
                     MTTNARLASEVEERKKIEEELRRSDLRHRTFLEASTDAIFLETLEGAILDCNTAATKI
                     YGYTKDEFLDLTVLDLVPLEIRDEIPRVIAAEIKARRFSIEAHNVRKNGEVFPCEVNT
                     RVVKMEGAELVAVFVRDITERRRAEAAQRRELEVNTALAATYAILVSPESSIRDITSV
                     ILKQALALTESAHGFVSKIDPESGENIGHTLTDMLGDQCGMSFEGEQVVFHPRPDGTY
                     PGLWGVPLNARRPFVSNDPIGHPASTGLPDGHIPISRFLSVPVMLGERLVGQISLANA
                     ARPYGDDDVDAITRLAEFYALAVQRWRVEERIKSLNESLTRNIIQIETANKELEAFSY
                     TVSHDLRAPLLTIGGYSKLLLKRYRQNLDEEAQKFLNIITTDSLRLEKMMEELLTLSR
                     MGYKQLELTDIDMTELARSVIGELERTSRNANTRIVVKALPMSRGDLLMIRQVFVNLL
                     SNSIKYTRPAKEPLIEIDGWTENGENVYRVRDNGV"
     gene            2826..4334
                     /locus_tag="PKJ16_05735"
     CDS             2826..4334
                     /locus_tag="PKJ16_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46523.1"
                     /translation="MRHLIIAIAAVLLLCPASAIASRPIIGVAGVEGGGAMGSQLAGL
                     MTGHLNRIVGVSGVFDQVNPALLRDQLARFDCLEASCMIRFARTAKISVIALGAVDDN
                     GGSVTVELAAYGIDAPYFGKVIYRYRAMIPTTGRRPTGQQLSYFTEEHAGIFVAELVR
                     RYRLPAAVMRDGSRAAVRFPERVDGSYDLYRFDEVPATDGGARPFRKVGTVTVENSAI
                     VRARPEGTDPADGDFILATHAEKARFLSEFNDGRKRELVLEKTGVADTLWTALFTVPA
                     SATMPLAAPILGYYRHGDFEGLALWALNVAPYLYVEVDGLMNPPEKYRADRRDVSNRT
                     LTHHYFGWYFMLVGGMSLFVDAFSHHYLDEASKFRGPQPLMGNGGTAAYLALICGGGG
                     HFYRGHRAWGYFYFHLDNIMLYMAIRELVGEERYNPETQSYERGAVNTGTGYALLGAF
                     GVIKIIELVHALLIKDNLRSGTVVEEEFAIEPLFMIDRERRMQAGVMCTFRY"
     gene            4316..5902
                     /locus_tag="PKJ16_05740"
     CDS             4316..5902
                     /locus_tag="PKJ16_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46524.1"
                     /translation="MHVPLLIASPERRAGGPSPVRFHLRRVALAALLALAHCAVAAAD
                     VPPEEERPIGGSVAIFARTAQDATMMALGNQSALHLKRVCNRLGRFFPVENNRLRAAD
                     RALTKRPADEDEYYRALAAVLKVDLYIVLSHYHVGPDIVGEMRIEPVSPNAAAQRRRV
                     RVRSRILKNVPLKLGRELAYLHEGVPVRGRIVRRLGGDRYAVTIGQWHGVEKGRRYPS
                     SVGEITLEETGRYESVARIAGDAARPGDPVVIDRPPDIRRITGELEETIARNAENRYG
                     LRETQLHGEDDRKRLVEGLCVINLGGNVCLPGYGAYLSTGYMGFAEPVPDIPGIALSA
                     TVFAAQLTLPEFLTGWRVNFFPWEKDENKTPPVHSLQIYLWSAIPLTFSAAYLDQLAV
                     QYKKFDRLPPFFMNHDEAAALFSLFIPGGGLFYKGYRLGGWSYFLSEMALVGYAVYWM
                     NDDTGDRSARTNGAYALAAFGVIKAIELVNAYFISPSYTVYSVEMEREYRNAELYFDL
                     RPEPEGRKGVTYRLGALYRM"
     gene            complement(5987..7099)
                     /locus_tag="PKJ16_05745"
     CDS             complement(5987..7099)
                     /locus_tag="PKJ16_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46525.1"
                     /translation="MITITGPRQSGKTTLARMAFPEKTYVNLEDMESRSFAIDDPKGF
                     LKKHHSGAIIDEFQNAPELASYIQVHVDESRRKGLFCLTGSRQFEVMNLASQSLAGRT
                     AMLRLLPFTFSELSHSTKKRHSDEFLFSGFYPRIYSDRLDPARAMEDYITTYLERDLR
                     TLSAIQDLHLFQKFMKLLAGRIGQILNVSSLASDCGASHTIITRWISLLETSYIIFLL
                     QPYHGNISKRLIKSPKVFFFDTGLAAHLLGIEKREHLEPHPLRGNLFENMVIADILKE
                     RFHHGLRNNCYFYRDSKGNEIDLCYETGHRLIIVEIKSGATITSEYMKGLNRFSGEFP
                     HLVNNAFLVYNGDTRDTRSGITICGPDEIARRLINE"
     gene            complement(7321..7785)
                     /locus_tag="PKJ16_05750"
     CDS             complement(7321..7785)
                     /locus_tag="PKJ16_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HNV46526.1"
                     /translation="MVHSLAAIQEIVASSRAAGTTVVLAEGCFDVLHVGHVRYLEDAR
                     RHGDLLIVSLNSDRTITELKGPGRPLVPEDERAEVIAALRCVDHVFIFDSSSSRELLA
                     AVEPDIYAKGTDYTPEGLIEREGLHEFRGRIVIVGDEKTHESTTMVRKLQTK"
     gene            complement(7790..8422)
                     /locus_tag="PKJ16_05755"
     CDS             complement(7790..8422)
                     /locus_tag="PKJ16_05755"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV46527.1"
                     /translation="MSRTSLIAIAAVACMLTALSCSTTIININRPAFDAMKTVAVMRF
                     ETAHGIPRNLATEAEEAFRGHFIGIGKSVVERDKLRAIMNEVEKSQAGMVSDPGRIGQ
                     LSGAGGLLFGNITQHGEDVRWVTYYETVKDKNDPKKSVRIEKKKQMKYFTFQTSVRLV
                     STSTGATILTMKNEYPERSYSIDNTMTLSRYREMVLNDMGRDLKKLLEKK"
     gene            8596..9498
                     /locus_tag="PKJ16_05760"
     CDS             8596..9498
                     /locus_tag="PKJ16_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46528.1"
                     /translation="MIQHLTDRVSVVFTEDGFTSGNCILVEDDVRLMIDSGCGRELDS
                     VRPEAVELLLCSHHHVDHIGGNDRFVNARILAHPLEAAAMQVAEKLIATDRWQELMDE
                     ENFFCVPEVNGAPNRLYLPWRVDGPLAEGQVVDCGATRIGVIHTSGHTAGHCSFHLLD
                     DDIVFIADICLSKVGPWYGGRDSSIDAFIASIDRIIDMKPATIVTGHLGSVLTENIPG
                     VLGEYRGRILNRESRILAYVRDHPSTINELAQRHFIYPAHPSLFVLYWEKSMLHNHLD
                     RLIALGEVARYDDGRYGPARVLGA"
     gene            9668..11335
                     /gene="hcp"
                     /locus_tag="PKJ16_05765"
                     /gene_synonym="priS"
     CDS             9668..11335
                     /gene="hcp"
                     /locus_tag="PKJ16_05765"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HNV46529.1"
                     /translation="MFCYQCQEAAGGTACTASGVCGKTADVADLQDLLVYTLSGVSTY
                     AAVLNANGIDTASADAAVTEGLFATITNANFDPRVFVERVFAGLALRERLREQWRAAG
                     FSGALPDAATWSADTEAQLVEKAASVGVLRTENEDVRSLRELVVYGVKGMAAYAHHAA
                     RLGANDPEIYAFMHRALAATLDDSLTADELVGLVLECGKFGVDVMALLDRAHTESFGA
                     PEITSVNLGVRGNPGILVSGHDLKDLEELLVQTEGSGVDVYTHGEMLPAHSYPAFKRF
                     AHFVGNYGNGWWRQKEEFESFRGPVLMTTNCLVPPRDSYRDRVYTTGLAGYPDMSHVA
                     APAGGGPKDFSAIIAHAKRCAPPVEIEQGALVGGFAHATVLSLADTVVDAVKGGAIKR
                     FFVMAGCDGRMKSREYYTAFAERLPADTVILTAGCAKYRYNKLDLGDIGGIPRVLDAG
                     QCNDSYSLAVIALALKDAFGLDDVNDLPISYNIAWYEQKAVIVLLALLYLGVKNIHLG
                     PTLPAFLSPAVASVLVERFGIAGIGAVDEDVAMFMGERDPDACAACS"
     gene            complement(11403..11645)
                     /locus_tag="PKJ16_05770"
     CDS             complement(11403..11645)
                     /locus_tag="PKJ16_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV46530.1"
                     /translation="MNCNRCGACCIAPSISSAIPGMPGGKPAGVRCIHLTADRTCGIY
                     DQRPLVCREFTPTPELCGASRDEALANLARLEVLTR"
     gene            complement(11642..11890)
                     /locus_tag="PKJ16_05775"
     CDS             complement(11642..11890)
                     /locus_tag="PKJ16_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46531.1"
                     /translation="MNHITRECGVIEDGSWYDEPIGWHSCGKECYEDTAAYCRRMGYR
                     YVGRNITCAVETALAIIVVTIVIGVLIVVRYVRRRRLR"
     gene            complement(12013..12201)
                     /locus_tag="PKJ16_05780"
     CDS             complement(12013..12201)
                     /locus_tag="PKJ16_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46532.1"
                     /translation="MTRIRTNLAARFTPSPGIKLAARFVVFGFAICSLSHRRVIAITS
                     PASIAAGRYWRTATDGGP"
     gene            12352..13434
                     /gene="carA"
                     /locus_tag="PKJ16_05785"
     CDS             12352..13434
                     /gene="carA"
                     /locus_tag="PKJ16_05785"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HNV46533.1"
                     /translation="MPTTAFLILEDGTEFTGTLFGHESETIGEVVFNTSMSGYQEVLT
                     DPSYNGQIVAMTYPMIGNYGVNPDDVESDRVQVAGFVVKEYSKTYSNFRATQSLGDYL
                     REARVSAIEGIDTRRLTLHIRDKGALRGGIFLDRTGAVDRLKAHPNMEGLDLTLNVAC
                     ATPYDFAPREKGTPHVAVFDFGVKTNILRLLRDSGFSVTVHPGSRPLREVLGEGADGV
                     FLSNGPGDPDAVKYARALVADIAKEKVPCFGICLGHQILALGLGGRTYKLKFGHRGGN
                     QPVKNLRTGSVEISSQNHGFAVDPESFANNSDIEITHRNLNDNTVEGFRHKSLPIFSV
                     QYHPEAAPGPHDSRYLFEEFRQLVAR"
     gene            13431..14606
                     /locus_tag="PKJ16_05790"
     CDS             13431..14606
                     /locus_tag="PKJ16_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV46534.1"
                     /translation="MTAHGAHDPGGAAEASRAPHEAHVRLARAFSSVFLIGPPMGEEL
                     VHIVSHLFTHEEAAVAARVPYYLPRSARTIARRVGRPVAEVAPLLDAMAARRVIVGTR
                     RGYALIPLIPGMFEYMLMPGEASDWHRTYGDLISRLFESGFTREYSTAAMPAIRTISV
                     QKTVEGKSHVVDADLVSRMIDAHEDLAVAKVCQCRQSARFSGRECARSAPEDGCLIFG
                     AFAASAAGNGSARRVSRDEMRAIVDDRWKKRLIFFAGNVDPASPNAICTCCDCCCHYV
                     EAITRYGAMATLSPPHYIALVDEARCTDCGLCVPACNTRAHTLVAKKHGYDASRCVGC
                     GLCVDACRRGAVTMAVNPSYRRPSRSFPRLMLRLAPAIFVSGLRVLIRRRRTAPNEA"
     gene            14726..15760
                     /locus_tag="PKJ16_05795"
     CDS             14726..15760
                     /locus_tag="PKJ16_05795"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HNV46535.1"
                     /translation="MKSYTHSTGTYIGKGGVEIFYQCWTVERPRAVVVIAHGVGEHSG
                     RYGHIINAMSGAGVSFYAIDHRGHGKSGGKMGHVESFMEYVYDLKLFMNFVKEDGKNA
                     PLFMLGHSMGGVIALKYALTYPEDIDALIASSAGMIPAVEIPKWKESLGLFFSKHLPG
                     FTMPSGLPSTFISHDMEIVRAYDNDPLVHDKVSARWYTEFLGTSQECLARASELRMPL
                     LVFHGTGDQIVDFKGSDRLFQAASSKDKSLHLFEGLYHETMNETPAERAKVLDVVSKW
                     IMAHCTKGAYAAAKKKPATVKPVAKKKGPAKKAASKKSPAKMKPAQRKPAAKKASVKK
                     KPAKKSGKRK"
     gene            15954..16184
                     /gene="vapB"
                     /locus_tag="PKJ16_05800"
     CDS             15954..16184
                     /gene="vapB"
                     /locus_tag="PKJ16_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HNV46536.1"
                     /translation="MQTAKLFINGRSQAVRLPREFQFSGTEVLIHRVGDAVLLVPRDK
                     PWEVFMHGLTSFSDDFLDAGRNQGVQKKRTSL"
     gene            16184..16600
                     /locus_tag="PKJ16_05805"
     CDS             16184..16600
                     /locus_tag="PKJ16_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV46537.1"
                     /translation="MCLLDTDICIYIIRKRPPEVLRALKRNYRAGLHVSSISVAELEY
                     GVEKSAFPEKNRVALVEFLSLFDIVAFTDADAAAYGTIRTALEKNGSPIGPLDLLIAA
                     QALSRNYTLVTNNTREFARIETLKIDNWAASPAEGK"
     gene            complement(16658..17506)
                     /locus_tag="PKJ16_05810"
     CDS             complement(16658..17506)
                     /locus_tag="PKJ16_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV46538.1"
                     /translation="MIINHNVSAIFAHRTLKFHDWSITKDVEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQIQGLRQAERNTEDGMSMIQTAEGYLQESQDILQRIRVLAVQAANGIY
                     TPEDRQQIQVEISELVDEIDRIASQAEFNKMKLLTGSFARLNPTASMWFHMGPNMHQR
                     ERVFIETMTSAALGIRNPTVLTFISLSTPGKANAVIGLADEALRIISKQRADLGAYFN
                     RLEHAAKGLMNAYENTQASESRIRDTDMAEQMSSFVRYQILTQAATAMLAQANTKSQT
                     VLQLLK"
     gene            17845..>18201
                     /locus_tag="PKJ16_05815"
     CDS             17845..>18201
                     /locus_tag="PKJ16_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV46539.1"
                     /translation="MRAVDTLPDENKIFVIDTNVVLYDYRSIYSFQEHTVIVPITLLE
                     EIDRFKRGNEIINYNAREFTRELDALIGDRLPGEGVPLPSGGMLAVSTDIRKDEKLGS
                     VFWEDSPDHRILARAYN"
BASE COUNT         3222 a         5589 c         5815 g         3575 t
ORIGIN      
        1 ccacgccgtt gtcgcgcacg cggtagacgt tctccccgtt ctcggtccag ccgtcgattt
       61 cgatcagggg ctccttggcg gggcgggtgt acttgatgga attcgagagg aggttcacga
      121 acacctggcg tatcatcagg aggtcgccgc gcgacatggg gagcgctttc acgacgatgc
      181 gcgtgttcgc gttgcgcgat gtccgttcca gctcgccgat caccgagcgc gcgagctcgg
      241 tcatgtcgat gtccgtgagc tccagctgct tgtatcccat ccgggagagc gtgagcagct
      301 cctccatcat cttttcgagg cgcaacgagt cggtggtgat gatgttcagg aacttctgcg
      361 cctcctcgtc gagattttgc cggtagcgtt tgagcaggag tttcgaatac ccgccgatcg
      421 tcaaaagcgg cgcgcgcagg tcgtgcgata cggtgtagct gaaggcctcg agctccttgt
      481 tggccgtctc gatctggatg atgttgcgcg tgaggctctc attgaggctc ttgatgcgct
      541 cctcgacgcg ccaccgctgc accgcgaggg cgtaaaattc ggccagccgc gtgatcgcgt
      601 ccacgtcgtc gtcgccgtat ggtcgcgcgg cattggccag cgagatctgg ccaaccagcc
      661 gctcgccgag catcaccggc acggacagga agcgcgagat ggggatatgg ccgtcgggga
      721 gccccgtgct cgccggatgc ccgatgggat cgttcgacac gaagggccgc cgcgcgttca
      781 gcggcacgcc ccagaggccc ggataggtgc cgtcggggcg cgggtggaaa accacctgct
      841 cgccctcgaa cgacatgccg cactggtccc ccagcatgtc ggtgagcgtg tggccgatgt
      901 tctcgccgct ctcggggtcg atcttggaga cgaagccgtg cgcgctttcg gtgagcgcga
      961 gcgcctgttt taggatgacg ctggtgatgt cgcggatgga cgattccggc gagaccagga
     1021 tcgcgtaggt tgccgcgagc gccgtgttca cctcgagctc gcgccgctga gcggcctcgg
     1081 ccctgcgccg ctcggtgatg tcgcgcacga agaccgccac cagctccgcg ccctccatct
     1141 ttaccacgcg cgtgttcacc tcgcagggga acacctcgcc gtttttgcgc acattgtgcg
     1201 cctcgatcga gaagcgccgg gccttgatct ccgccgcgat gacccgcggg atctcgtcgc
     1261 gtatctcaag gggcaccaga tcgagcacgg tgagatccag gaattcgtcc ttggtgtatc
     1321 catagatctt cgtggcggcg gtgttgcagt cgaggatggc gccctcgagc gtttccagga
     1381 agatggcgtc ggtggaggcc tcgaggaagg tgcgatgccg caggtcgctt cggcgcagtt
     1441 cctcctcgat cttctttcgc tcctccacct cgctggccag ccgcgcattg gtggtcatga
     1501 gttcgcgggt gcgctcgcgg acgaggtcct cgaggtgctc gcggtactgc tgaagctccc
     1561 gttgcgcctt acgccgctcg gtgatgtcgc gcgagacgcc caggaagtag gcgaagttcc
     1621 ggtcttcgtc gtacaccacc gagacgatca tctcgacggg gatccgcgcg ccgttcttgt
     1681 ggagcacctc catctccacg gtcatgggct tgatgtccgc cccccgcttg atgtcgggca
     1741 tgagcgcggc gagccgcttg cgcatgccgg gaaggtgctc cggagcgatg atcttgtcga
     1801 gcgattcgct catcgcctcc tcgacggaga tgccgcgcag ctgcctgatg gaggggctca
     1861 cgtagagacg cttgaggttc tcgtccatcg tccagatcac gtcgccgatg ttttcggcca
     1921 gcagccggta gcgccgctcg ctctcctgga gggcccgctc ggcggccttg cgctccgtga
     1981 tgtcctggat gaagccgttc acctgcagga cgttccccgc gtcgtcgcgc ttcacggcgc
     2041 tgttgttcga tatccagcgc tccgtgccgt ccttgtggat gatccggtgc tcgattgcgg
     2101 gcaacggctc ggcgaggatg cgcgcggtga aatccagcac gcgcgcgcgg tcgtccggat
     2161 ggatcatcgt gtaccagaga tcgggatcgg cgtagtattc ctccggcgcg tacccggtga
     2221 tggccaggca cttggggctg tgataggtgg tcaccgcgac gccgtcgatg tagctcacgc
     2281 tgtagatgta ctcgttgatg ttctggatga gcgtcttgta gcggtcctgg ctcttgcgca
     2341 gcgtctcttc ggtctgcagg cgcttctcga ccaccgtgat gttctggaac accaggcgct
     2401 ggatcttgat catcacgaca aaggagacga ccgtcaccag gagcgtcaac gacaccacgc
     2461 tcaggatgtg cgattgcacg agcgtgaggt ggggcaggag cgcggttttg ccgatctcga
     2521 aggccgacat gaagacgagg gttcccagca tgatgacggc caccggcagg atgatcgccc
     2581 tgcggtcgat aaagcgcatc atgagccggt tgatgccgcg aacgcccagg ggcggcgtga
     2641 tgcgctttaa tctcaatcgg atgtattttt tcatgatgcg ttcatgctgt ccgagaatcc
     2701 attgcccgca cacggatgta tcggaccgag ataaaaaaat caagcaaaac ttgacccgat
     2761 ggccatattc gggaaaaaga attgcccaaa tgcggcgagt gtgcgtttat gcgctcgggt
     2821 cccccatgcg acatctgata attgccatcg ccgccgtgct gctgctgtgt ccggcctccg
     2881 ccatcgcgtc gcgccccatc atcggggtcg ccggtgtcga ggggggcggc gccatggggt
     2941 cgcagctcgc gggcctcatg acgggccacc tcaaccgcat cgtgggcgtc tcgggggtgt
     3001 tcgaccaggt gaaccccgcg ctgttgcggg atcagctcgc ccgcttcgac tgtctcgagg
     3061 caagctgtat gatccgcttc gcgcgcaccg caaagatcag cgtgatcgcc ctcggcgccg
     3121 tggacgacaa cggcggctcg gtgaccgtcg agctcgccgc ctacggcatc gacgcgccct
     3181 atttcggaaa ggtgatctat cgctaccgcg cgatgatccc cacgacgggg cgccgcccga
     3241 cggggcagca gctgagctat ttcaccgagg agcacgcggg gatcttcgtg gcggagctcg
     3301 tgcgccgcta ccggctcccg gcggccgtta tgcgcgacgg atcccgcgcc gcggtgcgct
     3361 tcccggagcg cgtggacggc tcctacgatt tataccgctt cgacgaggtt cccgccacgg
     3421 acggcggcgc gcgccccttc cggaaggtcg ggaccgtcac cgtcgagaac agcgcgatcg
     3481 tccgcgcccg cccggagggc accgatccgg ccgacggcga cttcatcctg gcgacccatg
     3541 ccgagaaggc gcgcttcctg agcgagttca acgacgggcg caagcgcgag ctggtgctgg
     3601 agaagaccgg cgtcgcggac acgctgtgga cggcgctctt tacggtcccg gcgagcgcca
     3661 ccatgccgct cgcggcgccc atcctgggct actaccgcca cggcgatttc gaggggctcg
     3721 cgctctgggc gctcaacgtc gccccgtacc tctacgtcga ggtcgacggg ctcatgaacc
     3781 cgccggagaa gtaccgcgcg gaccggcggg acgtatcgaa ccgcaccctc acgcatcact
     3841 acttcggttg gtatttcatg ctggtgggcg gcatgtcgct gttcgtggac gccttctcgc
     3901 accactatct cgacgaggcg tcgaagttcc gggggccgca gccgctcatg ggcaacggcg
     3961 gcaccgcggc ctatctcgcg ctcatctgcg gcgggggcgg gcatttttac cgcggccatc
     4021 gcgcctgggg gtacttctat tttcacctgg acaatatcat gctgtatatg gccattcgcg
     4081 agctcgtagg ggaggagcgc tacaatcccg agacccagtc ctacgagcgc ggcgcagtga
     4141 acaccgggac ggggtacgcc ctcctggggg ccttcggcgt catcaagata atcgagctgg
     4201 tgcacgcgct cctcatcaag gacaacctgc gcagcggcac ggtcgtcgag gaggagttcg
     4261 cgatcgagcc gttgtttatg atcgatcggg agcggcggat gcaggcgggg gtgatgtgca
     4321 cgttccgcta ctgatcgcgt ctccggagcg gcgggctggc gggccgtctc ccgtccgctt
     4381 ccaccttcgt cgcgtcgcgc tcgcggcgct tcttgcgctt gcgcactgcg ccgtcgccgc
     4441 ggccgatgtc cctccggagg aggagcggcc gatcggcgga tcggtcgcca tcttcgcgcg
     4501 cacggcgcag gacgccacca tgatggcgct agggaaccag tcggccctgc acctgaagcg
     4561 cgtctgcaac cggctggggc gctttttccc ggtggagaac aaccggcttc gcgccgccga
     4621 ccgcgcgctc acgaaacgcc ccgccgacga agacgaatac tatcgcgctc tcgccgccgt
     4681 cctgaaggtg gacctctata tcgtgctgtc gcactatcac gtcggcccgg acatcgtcgg
     4741 cgagatgcgc atcgagccgg tatcgcccaa cgccgcggcc cagcgtcggc gcgtgcgcgt
     4801 gcgcagccgc atcctcaaaa acgtgccgct caagctgggg cgcgagctcg cctatttgca
     4861 tgagggcgtg cccgtgcgcg ggcggatcgt ccgccggctg ggcggcgacc gctacgccgt
     4921 gacgatcggg caatggcacg gcgtcgagaa ggggcggcgc tatccctcga gcgtcggcga
     4981 gatcaccctc gaggagaccg gccggtacga gtccgtcgcg cgcatcgcgg gggacgccgc
     5041 gcgccccggc gaccccgtgg tcattgatcg gccgccggac atccggcgca tcaccggcga
     5101 gctcgaggag acgattgcgc gcaacgcgga aaaccgctat ggcctgcgcg agacgcagct
     5161 gcacggcgag gacgatcgca agcgcctcgt ggaggggctc tgcgtcatca acctgggcgg
     5221 gaacgtgtgc ctgcccgggt acggcgccta tctctcgacc ggctatatgg ggttcgccga
     5281 gccggtgccg gatattcccg gcatcgcgct ctccgcgacg gtgttcgcgg cccaactcac
     5341 gctgcccgag ttcctgaccg gctggcgcgt caacttcttc ccctgggaga aggacgagaa
     5401 caagaccccc ccggtccaca gcctgcagat ctatctctgg tccgcgattc cgctcacctt
     5461 ctcggcggcg tacctcgacc aactggcggt ccagtacaag aagttcgatc gcctgccgcc
     5521 gttcttcatg aaccacgacg aggcggcggc gctcttctcg ctgttcattc caggcggggg
     5581 gctcttctac aagggatatc gactgggcgg atggtcgtat tttctgtcgg agatggcgct
     5641 ggtgggctat gcggtctact ggatgaacga cgatacgggc gaccggtcgg cgcggacgaa
     5701 cggcgcctac gccctcgccg ccttcggggt gatcaaggcg atcgagctcg tcaacgccta
     5761 cttcatcagc ccgtcgtata cggtgtattc ggtcgagatg gagcgcgagt accgcaatgc
     5821 cgagctctac ttcgatctgc gccccgaacc ggaaggacgc aagggcgtca cgtaccgtct
     5881 cggggccctg tatcggatgt gacgggctcc ggagctatcg tatcgcataa cgtgatcggt
     5941 gcgatcaggc gcgttccgta ttgcgggccg cattattgat cgctagttac tcgttgataa
     6001 ggcgccgtgc tatctcgtcg ggtccgcata tggttatacc gctcctcgtg tcccgcgtat
     6061 cgccgttgta aacgagaaac gcgttattca ccagatgggg aaattctccg gaaaaacggt
     6121 tcaatccttt catgtattcc gaggttatcg tcgcaccgct tttaatttct acgatgatta
     6181 accggtgccc ggtttcgtag cagagatcga tttcatttcc tttgctatcc cggtaaaagt
     6241 aacaattgtt tcgaagcccg tgatggaacc gctccttgag gatgtcggcg ataaccatgt
     6301 tctcgaacag attccccctg agcggatggg gctcgagatg ttcccgcttc tcaatgccaa
     6361 ggaggtgggc ggcgaggccg gtatcgaaaa aaaatacttt tggggatttg atcagccgct
     6421 tgctgatgtt gccatgatag ggctgaagca gaaagattat ataactcgtt tcaaggaggg
     6481 atatccatcg cgtgatgatg gtatggctgg ctccgcaatc gctggcgagc gatgagacat
     6541 tgagaatctg ccctattctc ccggcgagca gcttcatgaa tttctggaat aggtgcaaat
     6601 cctggatcgc cgaaagtgtt cgcagatcac gttcgagata cgtcgtaata tagtcttcca
     6661 tggcccgtgc ggggtccaaa cggtcgctgt aaatgcgcgg atagaatccc gagaagagaa
     6721 attcgtcgga atggcgtttt ttcgtgctgt gcgacagttc ggaaaaggtg aagggcagca
     6781 gcctcagcat ggccgtcctc cccgcgaggc tctggctcgc caggttcatg acttcaaact
     6841 gccggcttcc ggtgaggcaa aacagtcctt tccggcgcga ttcatccaca tggacctgaa
     6901 tgtacgacgc caattcgggg gcattctgga actcgtcaat aatcgctccc gagtggtgtt
     6961 tctttagaaa ccccttcggg tcgtcgatgg cgaacgaccg cgattccatg tcctctaaat
     7021 tgacgtacgt tttttcaggg aacgccatgc gggcgagtgt tgtttttccg gattggcgcg
     7081 gaccggtgat ggtgatcacg ggatattgtt tgagattctg gatggcgaat ttttgcaggg
     7141 ttcgagtgat tagcattatg gtgtaaattt aaactaatat tttaaattta caccataatg
     7201 acatatggga aaatttttgt caaatacgaa acatcggtat gattgagtac ttccgcacgc
     7261 tcttcaacag atgggcccga acgtgatgca tgccgcgtgt cggttgcgcc cgccgcgccg
     7321 tcacttcgtc tgcagcttcc gcaccatcgt ggtggattca tgggtcttct cgtcgcccac
     7381 gatgacgatg cgtcccctga actcgtgcag cccctcccgc tcgatgagcc cctccggcgt
     7441 gtaatcggtc cccttggcgt agatgtcggg ctccactgcc gcgaggagct cgcgcgacga
     7501 cgatgagtcg aagatgaaga cgtgatccac gcagcgcagc gccgcgatca cctccgcgcg
     7561 ctcgtcctcg gggaccaggg gccggccggg ccctttcagt tcggtgatgg tccggtcgga
     7621 attgagcgac acgatcagga ggtcgccgtg ccggcgggcg tcctcgaggt agcggacgtg
     7681 tcccacgtgg agcacgtcga agcatccctc cgcgaggacc acggttgtcc cggcggcgcg
     7741 gctggatgcg acgatctcct ggatggcggc cagcgagtgg accacggggc tatttcttct
     7801 ccaggagttt tttcaggtcg cgtcccatgt cgttgagcac catctcccgg tagcgcgaca
     7861 gagtcatggt gttgtcgatg gagtagctgc gctcggggta ttcgttcttc atcgtgagga
     7921 tggtcgcgcc cgtcgacgtg gagacgaggc gcacgctcgt ctggaatgtg aagtacttca
     7981 tctgcttctt tttttcaatc cgcacgctct tcttggggtc gttcttgtcc ttcaccgttt
     8041 cgtagtaggt gacccagcgc acgtcctcac cgtgctgggt gatgtttccg aagagcagtc
     8101 ccccggcgcc ggagagctgc ccgatgcgtc cgggatcgct caccatcccc gcctgggact
     8161 tctcgacctc gttcatgatc gccctgagct tgtcgcgctc cacgacgctt ttcccgatcc
     8221 cgatgaaatg cccgcggaag gcctcctccg cctcggtggc gaggtttcgg gggatgccgt
     8281 gggcggtctc gaagcgcatg accgcgacgg tcttcatcgc gtcgaaggcg gggcggttta
     8341 tgttgatgat ggtggtggag catgagagcg ccgtgagcat gcacgccact gcggcgatag
     8401 cgatcaaact ggttcgagac atgatgcgtt cctccttgcc ctatgcggcg tcgagcgccc
     8461 gctgatggag atacgctcgc acatgcgcgc ggaaatgcaa atgaaaaatc gggcccgcgc
     8521 gaatcttttc gaagttcttg acagccggcg cgtttcgggc ttccctgcgg caggcgagcg
     8581 gagagggcga agcgcatgat tcaacacctg acagatcgcg tgtcggtcgt gttcaccgag
     8641 gacgggttca cctccgggaa ctgcatcctt gtggaggacg acgtccggct catgatcgac
     8701 agcggctgcg gccgcgagct cgattcggtg cgtcccgagg cggtcgagct cctgctgtgc
     8761 tcgcaccatc acgtggacca catcgggggc aatgaccggt tcgtgaacgc gcggatcctc
     8821 gcgcatcccc tcgaggccgc ggccatgcag gtggccgaga agctcatcgc cacggaccgc
     8881 tggcaggagc tcatggacga ggagaacttc ttctgcgttc ccgaggtgaa tggggcgccc
     8941 aaccggctct atcttccttg gcgcgtggac ggcccgctcg ccgagggcca ggtggtcgat
     9001 tgcggcgcga cgcgcatcgg cgtcatccat acgtcggggc acaccgcggg gcattgctcc
     9061 ttccatctgc tcgacgacga catcgtgttc atcgccgaca tctgcctctc gaaggtgggg
     9121 ccctggtacg gggggcgcga ttcaagcatc gatgcgttta tcgcctccat tgaccgcatc
     9181 atcgacatga agcccgcgac gatcgtgacc ggccatctgg gatcggtgct cacggaaaac
     9241 atacccgggg tgctgggaga ataccggggc cgcatcctca atcgggagtc gcgcatcctg
     9301 gcgtacgtgc gggatcatcc ctccacgatc aatgagctgg cacagcgcca tttcatctat
     9361 ccggcgcacc ccagcctctt cgtgctctat tgggagaaga gcatgctgca caaccacctg
     9421 gaccgcctga tcgcgctcgg cgaggtcgcc cgatacgacg acggccgcta cggtcccgcg
     9481 cgcgttctcg gcgcatagcg acggtcgcgc ggccgccgcc cgccgcggcg ctcctgctga
     9541 attcacgcga atcgccccct taaaaaaatg acaccaaaac gcttgacagg attcggggaa
     9601 tcatgttagc agtccttatt gttgggaatc ctaacgatat atcatccttg ggaaaagggg
     9661 gaatcgcatg ttttgctatc aatgtcagga agcggccggg ggtacggcct gtaccgcatc
     9721 gggcgtgtgc gggaaaacgg ccgatgtggc cgatctccaa gatttgttgg tgtacaccct
     9781 gtcgggtgtt tcgacgtatg ccgcggtgct caacgcgaac gggatcgaca ccgcgagcgc
     9841 cgatgcggcg gtcaccgagg gcctgttcgc gaccatcacc aatgcgaatt tcgatccgcg
     9901 cgttttcgtg gagcgggtgt tcgccggatt ggcgcttcgc gagcgcttgc gcgagcagtg
     9961 gcgcgcggcc ggattctccg gcgcgctccc ggacgccgcg acctggtctg cggacaccga
    10021 ggcgcagttg gtcgaaaagg ccgcgagcgt cggagtgctg cgtacggaga atgaggacgt
    10081 ccgctcgctt cgggagctcg tcgtgtacgg cgtgaagggg atggccgcgt acgcccatca
    10141 tgccgcacgc ctcggcgcga acgacccgga gatctacgcc ttcatgcacc gggcgctcgc
    10201 ggcgacgctc gacgattcgc tgacagccga cgagctcgtg ggactggttc tggagtgcgg
    10261 gaagttcgga gtggacgtta tggcgctcct cgaccgcgca cacacggaat cattcggcgc
    10321 tcccgagata acgagcgtga acctcggggt gcggggcaat ccgggcattc tcgtgagcgg
    10381 ccacgacctg aaggacctcg aggagctcct ggtgcagacc gagggcagcg gcgttgacgt
    10441 ctacacccac ggcgagatgc tgcccgcgca ttcctatccg gcgttcaagc ggttcgcgca
    10501 tttcgtcggc aactacggaa acggttggtg gcggcaaaaa gaagaattcg agagctttcg
    10561 cgggccggtc ctcatgacca ccaattgcct ggtgccgccg cgcgattcgt accgcgatcg
    10621 cgtgtacacc accgggctcg ccggttatcc cgacatgtcg catgtcgccg cgcccgcggg
    10681 cggcggaccg aaggattttt cggcgatcat cgcccacgcg aagcgctgcg cgccgccggt
    10741 tgaaatcgag cagggggcgc tcgtgggggg attcgcccac gcgacggtcc tctcgctggc
    10801 cgacacggtg gtcgacgccg tgaagggagg cgccatcaag cgttttttcg tgatggccgg
    10861 ctgcgacggc cgcatgaaga gccgcgagta ttataccgcc ttcgccgaac ggcttcccgc
    10921 cgataccgtg atcctcacgg cgggatgcgc caagtatcgc tacaacaagc tcgatcttgg
    10981 ggatatcggc ggcataccgc gggtgctcga cgcgggccag tgcaacgatt cctattcgct
    11041 cgcggtcatt gctctcgccc tcaaggacgc cttcggcctc gacgacgtga acgatctccc
    11101 gatctcgtac aatatcgcct ggtacgagca gaaggccgtg atcgtgctgc tggcgctgtt
    11161 gtatctcggc gtaaagaaca tccacctggg gcccacgctt ccggcgtttt tatctcccgc
    11221 cgtggcgagc gttctcgtgg agcggttcgg gatcgccggt attggcgcgg ttgatgagga
    11281 tgtcgcgatg ttcatgggag aacgagaccc ggacgcgtgc gcggcgtgct cctgactgct
    11341 ttggaagcgt gcgttgttgc agggcgcgag ggggacaact cgggcgtccc cctcgcgtag
    11401 ttctatctcg tcagcacctc gagccgcgca aggttcgcga gcgcctcgtc gcgcgacgcg
    11461 ccgcagagct ccggcgtggg cgtgaattca cggcatacga gcgggcgctg gtcgtagatg
    11521 ccgcaggtgc gatccgccgt cagatggatg cagcggaccc ccgccggctt gccgcccggc
    11581 atgcccggta tggccgatga gatcgacggc gctatgcagc aggcgccgca gcggttgcag
    11641 ttcatcggag gcgcctccgg cggacgtatc gcacgacaat caacacgccg atgacgatgg
    11701 tgacgacaat gatggcgagc gccgtctcga cggcacaggt gatgttgcgc ccaacgtacc
    11761 ggtagcccat cctgcggcag tacgcggcgg tgtcctcgta gcattccttc ccgcaactgt
    11821 gccagccgat cggctcatcg taccaggagc cgtcctcgat cacgccgcac tcccgcgtaa
    11881 tgtggttcac ggcgccgtac tcgaccgtgg cgtgcagcgg agtttggcca acgagcgcac
    11941 acatgaccag caaaaaaggg gttattcccg cgcacagcga agcgcggatc ggggtgggcg
    12001 gggatggccg cattacgggc ccccgtccgt tgccgttcgc cagtaccgcc cggcggcgat
    12061 ggatgcgggc gaggtgatgg cgatcacacg gcgatgtgag agagagcaga tggcgaagcc
    12121 gaaaacgaca aaccgcgccg ccagcttgat gccgggcgac ggcgtgaagc gcgctgcgag
    12181 gtttgtgcgt atccgtgtca tgtgcgtgaa aaattgacgg tcccgatcat tcgggctagt
    12241 atgaacggtg gcgcgcaatg tcaatagttt taaccgcgga aagcatgctt gacaaatgtc
    12301 gccgctggcc cgtatggtgc cgataatcat caccctgtgc gagacgagac catgccaacg
    12361 acagccttcc tgatcctcga ggacgggacc gaatttaccg ggaccctgtt cggccacgag
    12421 tccgagacca tcggcgaggt ggtgttcaac acctccatga gcggctacca ggaggtgctc
    12481 accgacccct cgtacaatgg ccagatcgtg gccatgacct atcccatgat cggcaactac
    12541 ggcgtgaacc cggacgatgt cgagtccgac cgcgtgcagg tggcgggctt cgtggtcaag
    12601 gagtacagca agacctattc gaacttccgg gccacgcagt cgttgggtga ctacctgcgg
    12661 gaggcgcgcg tctcggctat cgagggcatc gacacccgcc gcctcacgct gcacatacgc
    12721 gacaaggggg cgctgcgcgg cgggatcttc ctggaccgta cgggggccgt cgatcgcctg
    12781 aaggcccatc cgaacatgga ggggcttgac cttacgctga acgtcgcctg cgcgacaccg
    12841 tacgatttcg cgccgcgcga gaagggcacg ccccacgtgg ccgtgttcga ttttggcgtg
    12901 aagaccaata tcctgcgtct gttacgcgat tccggatttt ccgtcacggt gcatcctggg
    12961 agccgtcccc tgcgcgaggt gctcggcgag ggcgccgacg gcgtctttct ctccaacgga
    13021 cccggcgatc ccgacgccgt gaaatacgcc cgcgcgctcg tcgccgatat cgcgaaggag
    13081 aaggtcccct gcttcggcat ctgcctgggg catcagatcc tcgcgctggg gctgggcggg
    13141 cgcacctaca agctcaagtt cggccatcgc ggcggcaatc agccggtcaa gaacctccgt
    13201 accggatcgg tggagatatc gtcccagaac cacggcttcg cggtcgatcc cgaatcgttc
    13261 gcgaacaatt cggacatcga aatcacgcac cgcaatctca acgacaacac cgtcgagggc
    13321 ttccggcaca aatcgcttcc catcttctcg gtgcagtacc acccggaggc ggcgccgggc
    13381 ccgcacgact cgcgctatct cttcgaggaa ttccgtcagc tggtcgcaag atgacggcgc
    13441 acggcgcgca tgatcccggc ggcgccgccg aggcgtctcg tgcgccgcat gaagcgcatg
    13501 tccggctcgc ccgggcgttc tcctcggtct ttctcatcgg gccgccgatg ggcgaggagc
    13561 tggtgcatat cgtctcccat ctctttacgc acgaagaggc cgccgtggcg gcgcgcgtcc
    13621 cgtattacct gccccgctcc gcgcggacga tcgcgcgccg ggtcggtcgc ccggtcgccg
    13681 aggtggcgcc gctgctcgac gcgatggccg cgcgccgggt gatcgtgggg acccggcgcg
    13741 gctacgcgct catcccgctt atccccggca tgttcgagta tatgctcatg cccggcgagg
    13801 cgagcgactg gcaccgcacc tatggcgatc tcatcagccg gctcttcgag tcgggcttta
    13861 cgcgcgagta cagcacggcg gcgatgcccg cgatccgcac catcagcgtc cagaaaaccg
    13921 ttgagggaaa gagccatgtc gtcgacgccg atctcgtgtc gcggatgatc gacgcgcacg
    13981 aagacctcgc ggtcgcgaag gtgtgccagt gccgccagtc ggcgcgcttc tcggggcggg
    14041 agtgcgcgcg ctccgcgccg gaggacggct gcctcatctt cggggccttc gccgcctcgg
    14101 ccgccggaaa cggcagcgcg cgccgcgtat cccgggacga gatgcgcgcg atcgtcgatg
    14161 accgctggaa gaagcgcctc atcttcttcg ccggcaacgt ggacccggcc agtcccaacg
    14221 cgatctgcac ctgctgcgac tgctgctgtc attacgtgga ggccatcacc cgctatggcg
    14281 cgatggccac gctctcgccg ccgcattaca tcgccctggt ggacgaggcg cgctgcaccg
    14341 actgcggcct ctgcgttccc gcgtgcaata cgcgcgcgca caccctcgtc gcgaagaagc
    14401 acgggtacga tgcgtcgcgc tgcgtcgggt gcggcctgtg cgtggacgcc tgccgtcggg
    14461 gcgccgtcac catggcggtc aacccgtcct atcgccgccc gtcgcgaagt tttccccggc
    14521 tcatgctgcg gcttgcgccc gcgattttcg tttcgggcct tcgcgtgctc atcaggcgcc
    14581 ggagaacggc cccgaacgag gcctaacggc ccggttttgc cggaaaaata ccgtcgcgga
    14641 tgaaaatgta tttgcgcata tcgccgccgc acgggtatca tgcccgataa tttttttacc
    14701 gaacacagcg gagggaatgc aacgcatgaa atcatacaca cacagtaccg ggacctatat
    14761 cggcaagggc ggcgtggaga tattctatca gtgttggacg gtcgagcggc cccgcgcggt
    14821 ggtggtcatc gcccacggcg ttggtgagca ctcgggccga tacggccaca tcatcaacgc
    14881 catgagcggc gcgggcgtga gcttctacgc catcgaccat cgcgggcacg gaaaatccgg
    14941 cggcaagatg ggccatgtcg agagcttcat ggaatatgtc tacgacctca agctcttcat
    15001 gaacttcgtg aaggaggacg ggaagaacgc cccgctgttt atgctggggc acagcatggg
    15061 cggggtgatc gccctcaagt acgcgctcac ctaccccgag gacatcgacg cgctcatcgc
    15121 gtcgtcggcc ggcatgatcc ccgccgtcga gatccccaag tggaaggagt cgctggggct
    15181 gttcttctcc aagcacctgc ccggattcac catgcccagc gggctgccct cgaccttcat
    15241 ctcgcacgac atggagatcg tgcgcgccta cgacaacgat cccctggtgc acgacaaggt
    15301 ctcggcgcgc tggtacaccg agttcctcgg aaccagccag gaatgcctgg cgcgcgcgtc
    15361 ggagcttcgc atgccgcttc tggtgttcca tggcacgggc gaccagatcg tcgatttcaa
    15421 gggttcggac cggctcttcc aggcggcgtc ctccaaggac aagtcgctgc acctcttcga
    15481 ggggctctat cacgaaacga tgaacgaaac gccagccgag cgcgcgaagg tgctggacgt
    15541 ggtctcgaag tggatcatgg cccattgcac gaagggcgcg tacgccgcgg cgaagaaaaa
    15601 acccgcgacg gtgaagcccg tcgccaaaaa gaagggcccg gcgaaaaagg ccgcttcgaa
    15661 gaaatccccg gcgaaaatga agccggcaca aagaaaaccc gccgcaaaga aggcctcggt
    15721 gaaaaagaag ccagcgaaga agtccggaaa gagaaaatag cggccaacgc ggcgggatcg
    15781 tacacggggc gggggaatgc atcctccgcc cttcgcatgt ccggacgcgc gtggtgatgg
    15841 cgcgtcgccg cgctgatccc catacgcatc accgacggca tgacgtgagg gggatttttt
    15901 tattgacaat agtatatatc gcgatatata ctattgatgc ggaggtggcg gccatgcaga
    15961 cggcaaaatt attcataaat gggcggagcc aggcggtgcg attgccgcgg gagtttcagt
    16021 tctccggaac ggaggtgctc atacatagag tgggggacgc ggtactgctg gtcccgcgtg
    16081 acaagccgtg ggaggtgttc atgcacgggc tcacctcatt ttccgatgat tttctggatg
    16141 ccgggcgcaa ccagggcgtt caaaagaagc ggacgtctct gtagtgtgcc tgctcgatac
    16201 cgatatctgc atttatatta tccggaagcg gcctcccgag gtgcttcgcg ccctgaagag
    16261 gaactatcgg gcggggttgc atgtttcatc gatttctgtc gcagagcttg agtatggggt
    16321 ggaaaagagc gcattcccgg agaagaaccg ggtggcgctc gtggagttcc tttcgctgtt
    16381 cgacatcgtg gcattcaccg atgccgatgc cgcggcatac gggacaatca ggacggcgct
    16441 ggagaagaac ggttcgccca tcggccccct ggaccttctg atcgccgcac aggcgctgtc
    16501 ccggaattat acgcttgtta ccaacaatac gcgcgagttc gcgcgaatcg agacattgaa
    16561 aatcgacaac tgggccgcgt caccggccga gggaaaataa aaaacgccgg gtgggaaagc
    16621 ccggcgtttt gaatgatact gcgatgttgg cccaatctta cttcaagagt tgcaatacgg
    16681 tttgcgactt ggtgttcgcc tgggcaagca tggccgtggc cgcctgcgtc aggatctggt
    16741 accgcacgaa cgacgacatc tgctccgcca tgtcggtgtc gcggatgcgg ctctccgatg
    16801 cctgcgtgtt ctcgtaggcg ttcatgaggc ccttcgcggc gtgctcgagc cggttgaagt
    16861 aggcgccgag gtccgcgcgc tgcttggaga tgatgcgcag cgcctcgtcg gccaaaccga
    16921 tcacggcgtt cgccttgccg ggcgtcgaga gcgagatgaa cgtgagaacg gtggggttgc
    16981 ggatccccag cgccgccgag gtcatggtct cgatgaacac gcgctcgcgc tggtgcatgt
    17041 tgggtcccat gtggaaccac atcgacgccg tcgggttaag acgggcgaac gacccggtga
    17101 gcagcttcat cttgttgaac tccgcctggg acgcgatacg gtcgatttca tcgacaagct
    17161 cggagatttc gacctggatc tgctgccggt cctcgggggt gtagatgccg ttggcggcct
    17221 gcaccgcgag cacccggatc cgctggagga tgtcctgcga ttcctgcagg tagccttcgg
    17281 cggtctggat catcgacatg ccgtcctcgg tgttccgctc ggcctggcgc aagccctgga
    17341 tctgcgagcg catcttctcg gacacggcca gccccgacgc atcgtcgccg gcgcggttga
    17401 tccgcatgcc cgaagagagc ttctcgacat ccttggtgat gctccagtcg tggaacttca
    17461 aggtgcggtg cgcgaagatc gcgctgacgt tgtggttgat aatcatgttt cctccttgaa
    17521 atttttcgct atcgtccttg atagcgggat gattcctttt tttacattcc cgcctgggtt
    17581 gacggagata taaaaattcg ttcactcaat gcttcgacat atcggcgatt ttccgtaaca
    17641 gcgaatatct ccaataaatc gtaaataaat acgattattc gtaaatacgg agttatttca
    17701 tgcatatttg gttcttgtca tttttttgtt gatggcggga cacggcacga tgccgcttcc
    17761 ggcgcgagat ggccgccgtt cgcactacac aaaaaaatta tgtcacatat attttccttg
    17821 atttgtatcg cgtacgatgc catcatgcgt gccgtggaca cgctccccga cgaaaacaag
    17881 atattcgtca tcgacaccaa cgtggtgctc tacgactacc ggagcatcta ctcgttccag
    17941 gagcacaccg tgatcgtccc gatcacgctg ctcgaggaga tcgaccgctt caagcgcgga
    18001 aacgagatca tcaactacaa cgcgcgcgag ttcacccgcg agctcgacgc gctcatcggc
    18061 gatcgtctgc ccggcgaggg cgtgccgctg ccgtccgggg gcatgctcgc cgtgagcacc
    18121 gacatcagga aggacgagaa gctcggaagc gtcttctggg aggacagccc cgaccaccgg
    18181 atcctcgcgc gggcctacaa c
//
LOCUS       DAORHF010000044        18149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2191_length_18149_cov_4.562065, whole genome shotgun sequence.
ACCESSION   DAORHF010000044 DAORHF010000000
VERSION     DAORHF010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18149
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_18149_cov_4.562065"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PKJ16_05820"
     CDS             complement(<1..219)
                     /locus_tag="PKJ16_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46540.1"
                     /translation="MGRLRDVTATEMPPSVAQQTSERDEISHVVQLRAFLDLPQHFNI
                     PLVDFPHGFFAFDEGVYPEKRYVDTVNFR"
     gene            389..1231
                     /locus_tag="PKJ16_05825"
     CDS             389..1231
                     /locus_tag="PKJ16_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46541.1"
                     /translation="MRHMLGVTLVVFIVSIAAVIAARHNFAGTWKTTWGDLVLTQEGD
                     AVRGNYGSGEKAGSIRGAVSGPRLSFSWREGRKRGRGYFDMVYDGKNVLIEGRWGNGD
                     SDTNGGAWKGHKEGGTFENTTQGGDPSPDTDTRAPLSKPVLPCGTSKCIRCPRPNIDP
                     LMPVNKWMAIMGNSDGPNSAMFINASNGVLYDVEYPAEFIKNGHYPCWQRCFTFEGSG
                     VLKVFTPDGRETRYIPWKIFDVGMRTYIFGPVDGHRQKWSYLDYPGWAPNGETDLTQR
                     CTCK"
     gene            1496..1945
                     /locus_tag="PKJ16_05830"
     CDS             1496..1945
                     /locus_tag="PKJ16_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46542.1"
                     /translation="MDGNSRIEEIQDVIRAVAADLGYLIYESAVYLKGENSKLSVKID
                     HLGGISHSDCEAYSRALSQRLDDEALLPNYMLEISSPGLTRKIRSLDEFIRFTGSKAK
                     VVFERAGKRESQTGIIAAVTGDEIELREGEGVIVIRYGEVVRANLDF"
     gene            1974..3380
                     /gene="nusA"
                     /locus_tag="PKJ16_05835"
     CDS             1974..3380
                     /gene="nusA"
                     /locus_tag="PKJ16_05835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HNV46543.1"
                     /translation="MSVNIFEALHQIATEKGIPKEFIEEIVEVAMLSAYKKQYGTIDN
                     VRVAFNRDNNSVAIVSRKIVVNRPRNPAEEIAYSEAKKIAPDAELGDEIDVSENPFVS
                     FGRIAAQTAKQVITQKLKEAEKSIIYNEFKDKEGELINGYFQRKTRDAIFVDLGKTEG
                     ILPAKEQSPLEHFKTGDRIKSLLLLVQKNSKGPSVVLSRAHTKFVEKLFEMEIPEIYD
                     GIMHIHAIVREAGMRTKVAVTSDRDDIDCVGACVGMKGMRIQSIVRELEGEKIDIIEY
                     SSERRTMAANALTPAKVKEVIEKSDGGVIAVVEDDQYKLAVGRNGHNARLSVRLCGFE
                     IDIKTEDQYREFLSSSESRALVEKLFATPAATDETPLDELPGLDARTIKLLEAGKIFS
                     VEDLVEASFEDLMKIDGIGEKTAVKIQEILREFVDFDEDEDEEEAESEAETGAGTETG
                     AEAGGDTAAEGEKEGTEE"
     gene            3385..5790
                     /gene="infB"
                     /locus_tag="PKJ16_05840"
     CDS             3385..5790
                     /gene="infB"
                     /locus_tag="PKJ16_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HNV46544.1"
                     /translation="MRATDIAAKNRVSVEDILSVCGELGIACENGEADLAEKDVFLID
                     KKIEVMKEKKAKETRMLLERKAEAQKGKIKLKRRVHVSKDIIKSGDEKGAAERPAEPA
                     KKTEPDKKPPVRERPSRPGERPAQQRPPRPGERPAQQRPPRPGERPRTGPAPAADRKN
                     EASGVAKDDGKRKKKEKGKEKEKEEKKRYGKQDEEKNIYYRKKKSSQPEVRAKEIGPP
                     KSIEITESIPVGDLAKKLNIKANEIIAKLMQLGVMATINQVIDAETAQILAGEYGTDV
                     KVVSLFDETVIKHEEEDLEADRTTRPPIVTVMGHVDHGKTKLLDAIRHTNVIDQEFGG
                     ITQHIGAYTVKVREKAVTFLDTPGHEAFTTMRARGASITDIVILVVAANDGIMPQTVE
                     ALNHAKDANVPIIVAINKIDLPEANPQKVRQELTAYGLIPEEWGGTTLIVNISAKQKQ
                     GIDDLLEMVLIQAEMLELTANNKLSARGTVIESKLDPGRGAVATILVQNGMLRVGDPY
                     VVGIYSGRVRAMFNDHSENIQEAGPSTPVEVLGLAGVPSAGDPFQVVENEKYAKLIAQ
                     KRQELKRIETAKKVRKVTLEDLNEMIKDGEVQELKIIIKGDVDGSVQALKESLEKLST
                     GEIRVKVVHSGTGGINESDVMLASASNAIIIGYHVRPNVRVSEIAEREKVSIKFYNII
                     YDVTNDVKAAMEGLLAPEIKEVVTGAGEVKQVFKISKVGSVAGSVVLSGKVERKNRLR
                     LVRDGVVIFDGQLASLKRFKDDASEVVAGQECGFNLENFNDIKEGDAFESYTIIKITR
                     KLE"
     gene            5790..6143
                     /gene="rbfA"
                     /locus_tag="PKJ16_05845"
     CDS             5790..6143
                     /gene="rbfA"
                     /locus_tag="PKJ16_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HNV46545.1"
                     /translation="MASYRREKLEELIRRVVAESLLKEVKDPRIGFVTVTRVSLSKDY
                     GTADVYVSVIGDESEKKLTMAGINSARSFIQHIVGSEVRLRIMPRIVFHLDTSIEEGV
                     NLVGLIDSANPRGDE"
     gene            6140..7093
                     /gene="truB"
                     /locus_tag="PKJ16_05850"
     CDS             6140..7093
                     /gene="truB"
                     /locus_tag="PKJ16_05850"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HNV46546.1"
                     /translation="MTGGARAAVDPRNAVLLLDKPAGMTSAAVVGAVQRILQQRKIGH
                     SGTLDKFATGLLVLCTGGATKLTRFFLDGDKRYEGIARLGVATDTDDGEGAVIARSVV
                     GEIDDAALASLRARFTGEMDQVPPRYSALKVSGRRASDLMRRGDDVALAPRRVTVYSL
                     EVRRVDGDPAALRLTAHCSKGTYIRSLVRDMGEALGCGAHLEALRRTASGSFGIEQAV
                     TLEELHGGADRAAGAGRGWVSPFDALGGFGVLAVDDEGAGRVANGAPFERRQVRSMEP
                     SSSQRPYRVIDGANNMIAIAEVDSDNWTIRYVNVFKEENKW"
     gene            7181..7438
                     /gene="rpsO"
                     /locus_tag="PKJ16_05855"
     CDS             7181..7438
                     /gene="rpsO"
                     /locus_tag="PKJ16_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HNV46547.1"
                     /translation="MTQKNGIIDQHKRHEKDTGSPEVQIALLTERINHLTEHFKVHKK
                     DHHSRRGLLKLVGQRRRLLDYLKKKDLAKYRELIKTLGLRK"
     gene            7612..9717
                     /locus_tag="PKJ16_05860"
     CDS             7612..9717
                     /locus_tag="PKJ16_05860"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HNV46548.1"
                     /translation="MNNNYSIQLGRETFAFNTGYLAKQADGAVLVSSGENMVFASVVA
                     AKDLVEGQDYFPLMVDYREKLYAAGLFPGGFIKRETRPSDRETLTSRLADRPLRPLFP
                     EDFMNEVQIIIYVMSSDGMTQPDVLAINAASGALTVSGIPFNGPVGAVRVGRIDGQFV
                     LNPTFDEAEKSEIDLVVAGTVKAVTMIEGLAQNVSEETMISAVEFAHENIKKICACQE
                     EMRAKCGKPTIGYTPVSADPELKKEIYDRYYGAVENLITIKDKKQREAAFEAIVKQAK
                     QELADRFPNTIKQAQGIIDGIDYTVMRKRILDESARADGRKLTEIRPIDIMLGLLPRA
                     HGSAVFTRGQTQSLGIVTLGTVADAQRVEALEGKSHKRFMLHYNFPPFSVGETGRVGG
                     TGRREIGHGMLAERALSYAVPDEEKFPYTIRVVSEILESNGSSSMATVCSGSLAMFNA
                     GVPLKSAVAGIAMGLVMEGGRYAILSDILGLEDHLGDMDFKVAGTATGITAFQLDIKI
                     EGITTEIMRTALNQAREGRMHILGEMSKACPEPSMQLSPYVPKMKMFMIDPDKIGAVI
                     GTGGKIIRGLIEECGADINIEDSGAVTVSSMSLDNIAMAEKKIRAIVEDIEVGKIYEG
                     TVKKCTDFGAFVEIVPGKEGLLHVSNMDFKRVNKVSDLMKVGDIVPVMVTAVDRSGKI
                     ELNRKEALKVLGKKSDDHN"
     gene            9704..10975
                     /locus_tag="PKJ16_05865"
     CDS             9704..10975
                     /locus_tag="PKJ16_05865"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HNV46549.1"
                     /translation="MTTIRKFRLDNGLTVILEPIEGVVSISVGLWARVGSRSETVEQY
                     GYAHFIEHMLFKGTGRYSAQDIAKIVDRVGGQHNAATNREYTCYYINVISDYLEMSVD
                     ILADMFYGSLFDAEELGKEKRVILEEIRMYEDSPDDLIHDLFMDCMMHQHPLGHSILG
                     TEESIKGARRDTLLSFFDTYYKNDNSILVITGRFDPDEAARLVEKYFSDNRRNGFVSP
                     VNGVPAPARIYRRHIDRDLEQVHFCLGAEGLRKRDDDRWALYTLSTVLGGNMSSRLFQ
                     NIREKEGLCYSIYSFHSSYADSGIFGIYCGTAPENFRRTLDLILDECVVMLRTRITEQ
                     ELADAKTYMKGNIALSFESTEVRMSQLARNEMIFGRYFSFEEIMAQIDAVGMDDFDRV
                     CNRILDGGPLTLVSLGRVNDAVEGEIRLQVS"
     gene            11086..11493
                     /locus_tag="PKJ16_05870"
     CDS             11086..11493
                     /locus_tag="PKJ16_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV46550.1"
                     /translation="MAGKGDIMNSTIGEGSVFEGKFYISGSLKIDGKFEGEIKTDEEL
                     VVGETGKVKTNIDAKSVVIAGTVVGNIRADNEVRLLETGRLLGDIVTPVLTVQRGVIM
                     QGNVTITGGQRKDPKKAVEESFEGGPKPHEKSK"
     gene            11541..12584
                     /locus_tag="PKJ16_05875"
     CDS             11541..12584
                     /locus_tag="PKJ16_05875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV46551.1"
                     /translation="MKRIFSKTYRFGRLSVSILMNEWIFLYRGNSRVIIKEIRTRDLF
                     TTKMKIAYGAAAVLLALSVFLFRGPAGEVADAPTPQQTAQNDEDRKNRILQSRDTDFS
                     TPKKKVKLVIRQHRVKKNESPARIAKKYGISLDTLCGSNNLNSYDYVKEGTVLKIPNK
                     DGVLYRVKKGEKMVSVAQKYKASLPRIIAENNIKNPDFFAPDSIIFIPDAKPLDIVPG
                     FLWPSMDRGITSGYGWRVHPIDRVRHFHLGLDMRSNYQWIKSTKYGQVTYTGWMGGYG
                     KTVIISHPGGWKSLYAHLSRIIVRTGQYVKQGQFVAKSGNTGNSTGAHLHFELIKNGH
                     HVNPYAYLVKKKR"
     gene            12748..13242
                     /locus_tag="PKJ16_05880"
     CDS             12748..13242
                     /locus_tag="PKJ16_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNV46552.1"
                     /translation="MVSIDVIQEKIRVVRGEKVMLDCDLARLYGVETKRLNEQVKRNS
                     GRFPERFMFQLTQDEFDILRSQFATSSWGGTRKPPFAFTEHGALMVSTVLNSAQAISM
                     SIYIIEAFVKMRSLVMSHAEILKRLDELEKNVGRHDRSLAQIINAIRQLMPTTEMPKK
                     KIGF"
     gene            complement(13340..15127)
                     /locus_tag="PKJ16_05885"
     CDS             complement(13340..15127)
                     /locus_tag="PKJ16_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNV46553.1"
                     /translation="MYQLDKPDNVVEFLENTVAKYPQRPFLGEKNKNGEYEWINYADF
                     GKRVDNCRAGLAQLGIKKDDAVGIISNNSTDWAVGFFATGGLAARYVPMYEAELVQVW
                     KYIINDADIKVLLVSKKEILNKIKDIISASPKLEKVILMESDGPDTLKELERKGAEKP
                     VASIKPDQSVIGVLIYTSGTTGDPKGVLLSQGNLSSNSHARIKMFSRFDEDERTLNIL
                     PWAHVYGLGEMITFMQIGASMGLAESATTIVDDIVKVKPTFLIAVPRVFNRVYDGLIT
                     MVNEKGGLAKKLFDMGVENGKKKRELAAQGKSSFGANFKFAIADKIVFSKVRAKLGGR
                     LKGSMTGSAAMNVEISHFFWDIGIPLYDVYGLTETTPAATMNGLDAWRIGSVGKAIDK
                     CKIVIDQSVVEPSAKDGEIIIYGPNVMQGYHKKPEATAAVMTSDGGFRTGDRGRLDAD
                     GFLYITGRIKEQFKLENGKYVFPAAIEEDIKLNHYIENAMIYGEGRAYNVCIVIPDFI
                     ALKKWAEKNNLPSEPAELIKRKECTDFLEKEIIDTIKGSYGSYELPKKFIFATEPFTL
                     ENGTLTQTMKLKRRVVFDKYKDQIEASYK"
     gene            complement(15337..17211)
                     /locus_tag="PKJ16_05890"
     CDS             complement(15337..17211)
                     /locus_tag="PKJ16_05890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV46554.1"
                     /translation="MGFLNLKTRAQKAAERDRRITVEGLCEIIKPGYRLFLSGGPATP
                     VHTIRHLAATDHPNLQDIEVIQLTTPSDFESLRAITPSKCRFKTFSIGENSARAMQMG
                     LLDIVPTTIAELPFMFLSGAVEIDVAIVQTSTPDKSGNVSLGTVNDVNRIAIANAAIA
                     IAEINPNVPVTHGDTRMSLSEFDYVIESAEPLLEFSAGPYDGTLDCIGRQIATLVEDG
                     STVSLSMGRLFDAVAAHLREKKNLKVWTHIVSDWIIGLSESGALASYDAMRRKSPVVT
                     TSCIGTRRLYDFVNGNPNVWLLPLFNSTHQQAMLGIKKLVSVLNVNKIDITGDSVFLS
                     ERDLQITGFDSKLNFALAATHSRGGKVIVGLRSTDRQGNSNIVISQPGSPIQNRCMLG
                     SIRYVVTEFGVATIFAKPIRERVLALIEVAHPDHRSRLFEEAKAGGLVFGDQVFVVEH
                     GINYPYALETMAPFKDGLAVKFRAIRPSDEDMMRRLFYQFSDEAKYLRYFTALRTMPH
                     SEMQQYLNVDYISTLSLVGIIQTRGVGRIIAECRYSQFAEENIYEMAFIVDEHYQGRG
                     IARYMLNRLLEIGRERGIERMSAYVLAHNAKMIAVLEKAAVPPRVIDLGEQLRYDYYL
                     "
     gene            17210..17380
                     /locus_tag="PKJ16_05895"
     CDS             17210..17380
                     /locus_tag="PKJ16_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46555.1"
                     /translation="MAATMRTVRKKYQQLNMHDGAGAAWRWDHEKTIEIIASPDFVDQ
                     RYPNGPMRGARP"
     gene            17377..>18149
                     /locus_tag="PKJ16_05900"
     CDS             17377..>18149
                     /locus_tag="PKJ16_05900"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV46556.1"
                     /translation="MIQNANFLCEIGCEEVPAGYVPPACAAIRALFEKRLADARIAYT
                     ELGVYATPRRFAVLASGMAERQAEEEVEVKGPSVKAAYDASGAPTKALEGFLKGNALA
                     LADVFPKATEKGEYLFARKTVAAVDTAAVLPAIVNDLVATVPFPKRMRWSDKAVTFPR
                     PVRYVLLMLNDRVVPFEISGIAAGKSTRGHYVQHNRMVELSSIAAYETTLEEHAVIVD
                     QERRRAMIADELAAAAKRLHGVLVEDEDLLDTVTYLVERP"
BASE COUNT         3740 a         5259 c         5814 g         3336 t
ORIGIN      
        1 tctgaaattg acggtgtcga cataccgttt ctcgggatag acgccctcat caaaagcaaa
       61 gaaaccatgc gggaaatcga ccagtgggat gttaaagtgc tgcgggagat caagaaaggc
      121 acgtaattga accacatgag aaatttcgtc cctttccgaa gtctgttgcg caacagaagg
      181 cggcatttcg gtagccgtca catcgcgcaa tcggcccatt cctggtaaaa aagtcctttt
      241 cgttggcctt aaatttcttc ctaatggaga cgtacggtgt ccacaaggac ttgccgtggt
      301 ggctctccca aaaccatctt gatttaccgc ttgctttttt cgctgtttcc ccccatggta
      361 tggcactacg attcgggagg accgcactat gagacacatg ctcggagtaa cgctcgtcgt
      421 cttcatcgtt tcgatcgccg ccgtcatcgc ggcgcggcac aatttcgccg ggacctggaa
      481 gaccacctgg ggcgatctgg ttctcacgca ggagggggac gccgtgcgcg gaaattacgg
      541 cagcggcgaa aaagcgggtt cgatccgggg ggccgtcagc ggaccgcgcc tgtcgttttc
      601 gtggcgcgag ggacggaagc gagggcgcgg gtacttcgat atggtctacg acggcaagaa
      661 cgtcctcatc gaggggcgat ggggcaacgg cgattcggac accaatggcg gcgcgtggaa
      721 gggccacaag gagggcggca ccttcgagaa tacgacgcag ggcggcgatc cttcgccgga
      781 cacggacacg cgcgcgccgc tctcgaagcc ggtgttgccc tgcgggacgt cgaaatgcat
      841 ccgttgcccg cggccgaata tcgaccccct catgccggtg aacaagtgga tggcgatcat
      901 ggggaactcc gacgggccca acagcgcgat gttcatcaat gcgtcgaatg gcgtgctcta
      961 tgatgtcgag tatcccgcgg agttcatcaa aaacggccat tatccctgct ggcagcgctg
     1021 tttcaccttc gagggttccg gcgtgctcaa ggtgttcacg cccgacggcc gtgaaacgcg
     1081 gtatattccc tggaaaatct tcgacgtggg catgcggacc tacatcttcg gtcccgtcga
     1141 cggccaccgc cagaagtgga gctatttgga ctatcccggg tgggcgccca acggcgagac
     1201 cgacctgacg cagcgctgta cctgcaagtg agggaagtct gcgacggatc gcgaaaagac
     1261 ccgcgcggca atgaaaaaaa acttgaaata tattccttct attttgatat ggtagattga
     1321 gtcgcggttt tgtcgtatgt gtcgtgggag cctcggttcc cttctcttga tatgatgtgc
     1381 gcatagaaag agagtggttg ctgccactct cttttttgga tatacgcgtc gccgcgcctc
     1441 ccggcgtccc gatggaaacg gggcgggggt acggtcgccg gacgatatgg gcataatgga
     1501 cgggaacagc cgcatagagg aaattcagga cgttatacgg gccgtggccg ccgatctcgg
     1561 atacctgatc tatgagtcgg cggtgtactt aaagggagaa aactccaagc tttccgtaaa
     1621 gatagaccac ctcggcggca tatcgcattc ggattgcgag gcgtacagcc gcgcgctatc
     1681 gcagcggctc gacgacgaag cgctccttcc caactacatg ctggaaattt cctccccggg
     1741 gctcacccga aagatacgga gcctggacga gttcatccgg ttcacgggat cgaaggcgaa
     1801 ggtggttttc gagcgcgcgg gaaagcggga gtcgcaaacg ggaatcatcg ccgccgtcac
     1861 gggagacgag atcgagcttc gcgagggcga gggcgtcatc gtcatccgct atggcgaggt
     1921 tgtccgggcg aacctggatt tctagttatc atttttcgcg aggattggtt gccatgagtg
     1981 tgaatatttt cgaggcgctc catcagatcg ccaccgagaa ggggataccc aaagagttca
     2041 tcgaggagat cgtcgaggtg gccatgctgt cggcgtacaa gaaacagtac ggcaccatcg
     2101 acaatgtgcg cgtcgccttc aatcgggaca acaacagcgt cgcgatcgtt tcacgaaaga
     2161 tcgtggtgaa ccggccccgc aatcccgccg aggagatcgc ctacagcgag gcgaaaaaga
     2221 tcgcgccgga cgcggagctc ggcgacgaga tcgacgtgag cgagaacccc ttcgtgtcct
     2281 tcggccggat cgccgcgcag acggccaagc aggtgatcac gcagaagctc aaggaggccg
     2341 agaagagcat catctacaac gagttcaagg acaaggaagg ggagctcatc aacggatact
     2401 tccagcggaa aacgcgcgac gcgattttcg ttgatttggg gaagaccgag gggatccttc
     2461 cggcgaagga gcagtcgccc ctcgagcact tcaagaccgg cgaccgcatc aagtcgctgc
     2521 tcttgctcgt gcagaagaac tcgaaggggc ccagcgtggt cctgtcccgc gcgcatacca
     2581 agttcgtgga aaagctcttc gagatggaga tcccggagat ctacgacggc atcatgcaca
     2641 tccacgcgat cgtccgcgag gcgggcatgc gcaccaaggt ggccgtgacc agcgaccgcg
     2701 acgacatcga ctgcgtgggc gcctgcgtgg ggatgaaggg catgcgcatc cagtccatcg
     2761 tacgggagct cgagggcgag aagatcgaca tcatcgaata ctcgagcgag cgtcggacga
     2821 tggcggctaa cgcgctcacc cccgcgaagg tcaaagaggt gatcgagaag tccgacggcg
     2881 gcgtgatcgc cgtggtcgag gacgatcagt acaagctcgc cgtggggcgc aacgggcaca
     2941 atgcgcgcct gtcggtgcgg ctctgcgggt tcgagatcga catcaagacc gaagaccagt
     3001 accgcgagtt cctgagctcc agcgaatcgc gcgcgctggt ggagaaactc ttcgcgaccc
     3061 cggcggccac cgacgaaacg ccgctcgacg agcttccggg actcgacgcg cgcaccatca
     3121 agctgcttga ggccggcaag atcttctccg ttgaagacct ggtcgaggcc tcgttcgaag
     3181 atctgatgaa gatcgacggt atcggcgaaa agaccgccgt gaagatacag gaaatcctcc
     3241 gcgaattcgt cgatttcgac gaggacgagg acgaggaaga agcggaatcg gaagcagaaa
     3301 ccggggccgg gacggagacg ggagccgagg cgggcgggga caccgctgcg gaaggggaaa
     3361 aggaagggac cgaggaataa ccacatgagg gcaaccgata tagctgcaaa aaacagggtc
     3421 tccgtcgagg acatcctgag cgtctgcggg gagctgggca tcgcctgcga gaacggcgag
     3481 gccgatctgg ccgaaaagga cgtgttcctg atcgataaaa agatcgaggt gatgaaggag
     3541 aagaaggcca aagagacccg catgctcctc gaacgcaagg cggaggccca gaagggcaag
     3601 atcaagctca agcgccgggt gcacgtctcc aaggacatca tcaaatcggg agacgagaag
     3661 ggtgcggccg agcggcccgc cgagccggcc aaaaagacgg agccggacaa aaagcccccg
     3721 gtccgggaac gtccctcgcg ccccggcgaa cggccggcgc agcagcggcc accgcgtccc
     3781 ggcgagcggc cggcgcaaca gcgtcccccg cgtcccggcg agcgaccccg caccgggccc
     3841 gccccggccg ccgatcgcaa gaacgaggcc agcggcgtcg ccaaggacga cggcaagcgg
     3901 aaaaagaagg agaagggcaa ggaaaaagag aaagaggaaa agaagcgata cggcaagcag
     3961 gacgaagaga agaacatcta ttatcgcaaa aagaaatcct cccagccgga ggttcgcgcc
     4021 aaggagattg gtccgccgaa gtcgatcgag ataaccgaga gcattcccgt cggcgacctg
     4081 gccaagaagc tcaacatcaa ggccaacgag atcatcgcca agcttatgca gctcggggtg
     4141 atggcgacca tcaaccaggt gatcgacgcc gagaccgcgc agatccttgc aggggagtac
     4201 ggcaccgacg tgaaggtggt ctcgctcttc gacgagaccg tcatcaaaca cgaggaggag
     4261 gacctcgagg cggaccgcac cacgcgcccg cccatcgtga ccgtgatggg ccacgtcgac
     4321 cacgggaaaa cgaagctcct ggacgcgatc cggcacacca atgtgatcga ccaggagttc
     4381 ggcggcatca cgcagcacat cggcgcctac accgtcaagg tgcgcgagaa ggccgtcacc
     4441 tttctcgata ctcccggcca cgaggccttt accacgatgc gggcgcgcgg cgccagcatc
     4501 accgacatcg tcattctggt cgtggccgcg aacgacggca tcatgccgca gacggtcgag
     4561 gcgctcaacc acgcgaagga cgcgaatgtc ccgatcatcg tcgccattaa taaaattgac
     4621 ctgcccgagg cgaacccgca gaaggtgcgc caggaactca ccgcctatgg gctcattccg
     4681 gaggagtggg gcgggacgac gctcatcgtg aacatcagcg cgaagcagaa gcagggcatc
     4741 gacgacctgc tcgagatggt cctcatccag gcggagatgc tggagctcac ggcgaacaac
     4801 aagctctcgg cgcgcgggac cgtcatcgaa tccaagctcg atccggggcg cggcgccgtg
     4861 gcgaccatcc tggtgcaaaa cggcatgctg cgcgtgggcg atccgtacgt ggtgggcatc
     4921 tactccggcc gcgtccgcgc catgttcaac gatcacagcg agaatatcca ggaggcgggc
     4981 ccgtcgacgc cggtcgaggt gctgggactc gcgggcgttc cgtccgcggg cgatcccttc
     5041 caggtggtgg agaacgagaa gtacgccaag ctcatcgccc agaagcggca ggagctcaag
     5101 cgcatcgaaa ccgccaagaa ggtccgcaag gtcacgctcg aggacctcaa cgagatgatc
     5161 aaggacggcg aggtccagga gctcaagatc atcatcaagg gcgatgtgga cggctccgtg
     5221 caggcgctca aggaatcgct ggagaagttg tccaccggcg agatccgcgt gaaggtggtc
     5281 cactccggca ccggcggcat caacgagtcc gacgtgatgc tggcctcggc ctccaacgcg
     5341 atcatcatcg gttatcacgt gcggccgaac gtgcgcgtgt ccgagatcgc cgagcgcgag
     5401 aaggtgtcca tcaagttcta caatatcatc tacgacgtga ccaacgacgt gaaggccgcg
     5461 atggagggcc tgctcgcgcc ggagatcaag gaggtcgtga ccggcgccgg cgaggtgaaa
     5521 caggtcttca agatttccaa ggtcgggtcc gtggccggtt cggtggtcct ctccggcaag
     5581 gtcgagcgaa aaaaccgcct gcgtctcgtc cgcgacggcg tggtcatctt cgacggccag
     5641 ctcgcgtcgc tcaagcgctt caaggacgac gcgtccgagg tcgtggcggg ccaggagtgc
     5701 gggttcaatc tcgagaactt caacgatatc aaggaaggcg acgccttcga gagctatacc
     5761 atcattaaga tcacgcggaa gctggaatag tggcgtcgta cagaagggaa aagctcgagg
     5821 agctcatccg ccgcgtggtc gccgagtcgc tgctcaagga ggtcaaggac ccccgcatcg
     5881 gcttcgtgac ggtcacgcgc gtgtcgctga gcaaagacta cggcaccgcc gacgtctacg
     5941 tctcggtgat cggcgacgaa agcgagaaga agctcaccat ggcggggatc aactccgcgc
     6001 gcagcttcat ccagcacatc gtggggagcg aggtccggtt gcgcatcatg ccccggatcg
     6061 tctttcacct cgacacctcg atcgaggagg gcgtgaacct ggtgggcctc atcgacagcg
     6121 cgaacccgcg cggggacgag tgaccggggg ggcgcgcgcg gccgtcgatc cgcgaaacgc
     6181 cgtgctcctg ctggacaagc cggcgggaat gacctcggcg gcggtggtgg gcgccgtgca
     6241 gcggatcctg cagcagcgga agatcggcca ctcgggcacg ctggacaagt tcgcgacggg
     6301 gctgctggtg ctgtgcaccg gcggcgcgac caagctcacg cgcttttttc tcgatggcga
     6361 caagcgctac gaggggatcg cgcggctcgg cgtggcgacc gataccgatg acggcgaggg
     6421 cgcggtgatt gcgcgctccg tcgtgggtga gatcgacgat gcggcgcttg cgtcgcttcg
     6481 ggcgcgattc accggcgaga tggaccaggt cccgccgcgc tattcggcgc tcaaggtgtc
     6541 cgggcggcgg gcgtccgatc tcatgcggcg gggcgacgat gtcgcgctgg cgccgcggcg
     6601 ggtgacggtg tactcgctcg aggtgcggcg cgtcgacggc gatcccgcgg cgctgcgcct
     6661 gacggcgcac tgctcgaagg gcacctatat ccggtcgctg gtgcgcgaca tgggcgaggc
     6721 gctggggtgt ggggcgcacc tcgaggcgct tcgtcggacg gcctcgggat cgtttggcat
     6781 cgagcaggcg gtcacgctcg aggagcttca cggcggtgcc gatcgcgccg caggcgccgg
     6841 tcgcggctgg gtgagcccgt tcgatgcgct gggcggtttc ggcgtcctgg ccgtggacga
     6901 tgagggcgcg gggcgcgtgg ccaacggagc gcccttcgag cgccgacagg ttcggtcgat
     6961 ggagccatca tcatcccagc ggccgtatcg ggtgatcgac ggcgccaata atatgattgc
     7021 aattgcggag gttgattccg acaattggac aatacgatac gtgaacgtct tcaaagaaga
     7081 aaacaaatgg tagtgattta ccgagacgat cgccagcgcg cgacaccgcc ggattcgaat
     7141 caaccatcaa tcatactgaa taaatgaggt acacgcggtt atgactcaga agaacgggat
     7201 cattgatcag cacaagcggc acgagaagga cacgggatcg cccgaggtcc agattgcgct
     7261 gctcaccgaa cggatcaatc atctgaccga gcatttcaag gtgcataaaa aggaccacca
     7321 ctcccggcgg ggccttctca agctggtcgg gcagcgacgc aggcttctcg attatctgaa
     7381 aaaaaaggac ctggcgaaat accgggaact aatcaagacg ctcggcctgc ggaagtaatt
     7441 cccgcccggg gctcacgcac ccgcttcggc ggcgcgggcg gatgggcggt gcgcgcaccg
     7501 cgtatccgct gccgggcgct ccggcgggcg catgcacaaa cgacgagacg gtccccggcg
     7561 cgcaggcgat ccgcggggcc gccgccaacc aaacacatat gagggtatcg aatgaacaat
     7621 aattacagca tacaactcgg tcgcgagacc tttgcattca acaccgggta tctggccaag
     7681 caggccgatg gcgccgtcct ggtgtccagc ggcgagaaca tggtgttcgc gtcggtggtc
     7741 gcggccaagg acctggtcga gggccaggac tactttccgc tgatggtcga ctatcgcgag
     7801 aagctctatg ccgcgggtct gttccccggc ggtttcatca agcgcgagac gcgcccgagc
     7861 gatcgggaga cgctcaccag ccggctcgcc gaccggccgc ttcgaccgct cttccccgag
     7921 gacttcatga acgaggtcca gatcatcatt tacgtgatgt cctccgacgg catgacgcag
     7981 cccgacgtgc tggccatcaa cgcggcatcc ggggcgctga cggtgtcggg cattcccttc
     8041 aacgggccgg tgggcgccgt gcgggtgggg cgcatcgacg ggcagttcgt gctcaatccg
     8101 accttcgacg aggcggagaa gagcgagatc gacctggtgg tcgccgggac cgtgaaggcg
     8161 gtcacgatga tcgagggatt ggcccagaac gtgagcgagg agacgatgat cagcgccgtc
     8221 gagttcgcgc acgagaacat caagaagatc tgcgcctgcc aggaggagat gcgcgcgaag
     8281 tgcggcaagc cgaccatcgg ctacacgccc gtcagcgcgg acccggagct caagaaggaa
     8341 atctacgacc gctattacgg cgcagtggaa aatctcatca ccatcaagga caagaagcag
     8401 cgcgaggccg cctttgaggc gatcgtcaag caggcgaaac aggagctcgc cgatcgcttc
     8461 cccaacacca tcaagcaggc gcagggcatc atcgacggca tcgactacac agtgatgcgc
     8521 aagcgcatcc tcgacgagag cgcccgcgcc gacggacgga agctcaccga aatccggccc
     8581 atcgacatca tgctggggct cctcccgcgc gcgcacggct cggccgtgtt cacccgcggg
     8641 cagacgcaga gcctgggcat cgtcacgctg ggcaccgtgg ccgacgcaca gcgcgttgag
     8701 gccctcgagg gcaagagcca caagcgcttc atgctgcact acaacttccc gccgttctcg
     8761 gtgggcgaga ccggccgcgt gggcggcacc ggacgccgcg agatcggcca cggcatgctc
     8821 gccgagcgcg cgttgagcta tgccgtgccg gacgaggaga agttccccta taccatccgc
     8881 gtggtctccg agatcctcga gtccaacggc tcgtcgtcca tggccacggt ctgctcgggg
     8941 tcgctcgcga tgttcaatgc cggcgtcccg ctcaagtctg cggtcgcggg catcgcgatg
     9001 ggcctggtga tggagggcgg ccggtacgcg atcctctccg acatcctggg acttgaggat
     9061 cacctgggcg acatggactt caaggttgcg gggaccgcga ccggcatcac cgccttccag
     9121 ctcgacatca agatcgaagg aatcaccacc gagatcatgc gcacggcgct gaaccaggcg
     9181 cgcgagggtc ggatgcacat tttgggcgag atgtccaagg cctgcccgga gccgtccatg
     9241 cagctttcgc cgtatgtgcc caagatgaag atgtttatga tcgatcccga caagatcggg
     9301 gcggtcatcg gcaccggcgg caagatcatt cgcggcctca tcgaggagtg cggcgctgac
     9361 atcaacatcg aagacagcgg cgccgtgacg gtctcgtcca tgagcctgga taatatcgcg
     9421 atggccgaga agaagattcg cgccatcgtc gaggacatcg aggtgggcaa gatctacgag
     9481 gggaccgtga agaagtgcac cgatttcggc gcgttcgtgg agatcgtccc cggcaaggag
     9541 gggctgctcc acgtctcgaa catggacttc aagcgcgtaa acaaggtaag cgacctcatg
     9601 aaggtgggcg acatcgtacc ggtgatggtc acggcagtgg accgctccgg gaagatcgag
     9661 ctcaaccgca aggaagcgct caaggtgctg ggcaagaagt ccgatgacca caattaggaa
     9721 atttcgcctg gataacgggc tcaccgttat cctcgagccg atcgaggggg tggtgtccat
     9781 ctccgtgggc ctgtgggccc gggtcggctc gcgcagcgag accgtcgagc agtacgggta
     9841 tgcgcacttc atcgagcaca tgctcttcaa ggggaccggg cgctactcgg cccaggacat
     9901 cgcaaagatc gtggaccggg tgggcggtca gcacaatgcc gcgaccaatc gcgagtacac
     9961 ctgctactat atcaacgtga tctcggatta tctggagatg tccgtggaca tcctcgcgga
    10021 catgttctac ggctcgctct tcgacgcgga ggagctgggc aaggagaagc gcgtgatcct
    10081 cgaggagatc cgcatgtacg aggattcgcc cgacgacctc atccacgacc tgttcatgga
    10141 ctgcatgatg caccagcatc ccctgggcca ctcgatcctt ggcaccgagg agagcatcaa
    10201 gggcgcacgg cgcgatacgc tcctctcgtt tttcgacacc tattacaaga acgacaacag
    10261 catcctggtg attaccggga ggttcgatcc cgacgaggcg gcgcgcctcg tggagaaata
    10321 cttctccgac aaccggcgaa acgggttcgt ctccccggtc aacggcgttc ccgcgcccgc
    10381 gcggatctac cgcaggcaca tcgaccgcga cctggagcag gtccactttt gccttggcgc
    10441 ggaggggttg cgcaagcgcg atgacgatcg ctgggccctg tacaccctga gcacggtcct
    10501 gggcggcaat atgtcgtcgc ggcttttcca gaatatccgg gaaaaggagg ggctgtgcta
    10561 ttccatttat tcattccatt cctcctacgc cgatagcggc atcttcggaa tctactgcgg
    10621 cacggccccg gagaacttcc gtcggacgct cgacctcatt ctggacgagt gcgttgtgat
    10681 gttgcgcacg cgcatcaccg aacaggagct tgccgacgcc aagacgtaca tgaaagggaa
    10741 tatcgccttg agcttcgaga gcaccgaggt gcgcatgagc cagttggcgc gcaacgagat
    10801 gatattcggg cgttatttca gcttcgagga gatcatggcg cagatcgacg cggttggcat
    10861 ggacgatttc gaccgggtgt gcaatcggat cctcgatggc ggtccgctca ccctggtatc
    10921 gctcggccgg gtcaatgacg cggtcgaagg ggagattcgg cttcaggtgt cataaaattt
    10981 atccgctcga ccaaaaacgg gttgcattaa tcgcgcccgg atcgatacgc tgtttcggga
    11041 cggagccggt tccatcgtag cgaatattgt tgtctggagg cttccatggc gggaaagggc
    11101 gatattatga acagcaccat cggagagggc tccgtcttcg agggtaagtt ctatatcagc
    11161 ggttccctga agatcgacgg caagttcgag ggcgagatca agaccgacga ggagctcgtg
    11221 gtcggggaga ccggcaaggt caagaccaat atcgacgcca aatcggtcgt gattgccggt
    11281 acggtggtcg ggaatattcg ggccgacaat gaggtccgcc tgctcgagac ggggcggctt
    11341 ctgggcgata tcgtcacgcc ggtgcttacc gtgcagcggg gcgtgatcat gcaggggaat
    11401 gtcaccatca ccggcggaca gcggaaggat ccaaaaaagg ccgttgaaga gtccttcgag
    11461 ggcggaccca agccccacga aaaatctaaa taaagataca acggtttccg atattctgag
    11521 tacaagcacc agcgagtgtt atgaagcgta tcttctctaa aacataccgg tttggccgcc
    11581 tcagcgtatc cattctcatg aatgaatgga tctttctgta tcgcggcaac agccgcgtga
    11641 tcatcaaaga aatacgcacg cgcgatctgt tcacgaccaa aatgaagatc gcctacggag
    11701 cggccgctgt cctactggcg ctctcggtgt ttctgttccg agggcccgcc ggcgaggttg
    11761 cggatgcccc caccccgcag cagacggcgc agaacgatga ggatcggaaa aaccggatcc
    11821 tccagtcgcg cgacaccgat ttctccaccc cgaagaaaaa ggtgaagctg gtgatccgcc
    11881 agcatcgcgt gaaaaagaat gaaagccccg ctcgcatcgc gaaaaaatac ggcatatcgc
    11941 ttgataccct gtgcggcagc aacaacctca attcgtacga ttatgtgaag gaaggcaccg
    12001 tgctgaagat ccccaacaag gacggtgtcc tgtatcgggt caaaaaaggc gaaaagatgg
    12061 tgtccgtggc ccagaaatac aaggcctcgc tcccgcggat catcgcggaa aacaatatca
    12121 agaacccgga cttttttgcg cccgactcca tcatcttcat tcccgacgca aagccgctgg
    12181 acattgtccc cggatttctc tggccgtcca tggaccgcgg tatcaccagc ggctatggat
    12241 ggcgcgtcca tccgatcgac cgcgtccgcc atttccatct cgggctcgat atgcgctcca
    12301 attaccagtg gatcaagtcc accaagtacg gccaggtgac ctataccggg tggatgggcg
    12361 gttatgggaa gacggtcatc atctcgcatc cgggcggctg gaagtcgctc tatgcgcacc
    12421 tctcgcgcat tatcgtgcgc accggacagt atgtaaagca ggggcagttt gtcgccaaga
    12481 gcggcaatac cggcaactcg accggcgcgc acctccattt cgagctcatc aagaacggcc
    12541 accatgtgaa cccctacgcg tacctggtca aaaagaagcg ctaagaccgc gcggcttttc
    12601 ctcgcagaaa aaatttcatc aatcggcatg gcggaccgta ttcttttata aatgcggtcg
    12661 attcgctgtc ccttccgcat tgctatgcca acgtgagcgc atgccgcaga cggagcggaa
    12721 acgacagcgt ggatgagggt gtgtgtcatg gtgagtatcg atgtaatcca ggagaagatc
    12781 cgtgtcgtgc gcggcgaaaa ggtcatgctc gattgcgacc tcgcgcggct gtacggtgtc
    12841 gagacaaaac gactgaatga gcaggtgaag cgtaacagcg ggaggtttcc cgaacggttt
    12901 atgtttcagc tgacccagga tgaatttgat atcttgaggt cgcaatttgc gacatcaagt
    12961 tggggcggaa cacgaaaacc gccctttgcc tttaccgagc acggcgccct catggtgtcc
    13021 acggtgctca atagcgctca ggcgatttcg atgagtatat acatcatcga ggcgtttgtg
    13081 aagatgcgct cactcgtcat gtcgcatgcg gaaatactga aacgcctcga tgagcttgaa
    13141 aaaaacgtgg gcaggcacga tcgttcgctc gcacagatca tcaacgccat tcggcagctt
    13201 atgccgacga ccgaaatgcc aaaaaagaaa atcggtttct aggggcttcg cattccttcc
    13261 gtttgagctc tctgtatatg gtaaacgggc cgcggcaaaa ccgcggcccg tatggtgaat
    13321 catcgcacga aacgtcgatc tacttgtagc tggcctcgat ctggtccttg tacttgtcga
    13381 agaccacccg gcgtttcagt ttcatcgtct gcgtaagcgt cccattctcg agggtgaacg
    13441 gctcggttgc gaagatgaac ttcttgggga gctcgtagct gccgtagctt cccttgatgg
    13501 tgtcgatgat ctccttttcg aggaagtccg tgcactcctt ccgcttgatg agttcggccg
    13561 gttctgaggg gaggttgttc ttctcggccc acttcttgag cgcgatgaag tccgggatca
    13621 cgatgcacac gttgtacgcg cggccctcgc cgtagatcat cgcgttttcg atgtagtggt
    13681 tgagcttgat gtcctcctcg atggcggccg ggaagacgta tttgccgttc tcgagcttga
    13741 actgctcctt gatgcgcccg gtgatgtaga ggaagccgtc cgcgtcgagg cggccgcggt
    13801 cgccggtgcg gaagcctccg tcggaggtca tcacggccgc ggtggcctcg ggcttcttgt
    13861 ggtagccctg catcacgttg gggccgtaaa tgatgatctc gccgtccttc gcgctcggct
    13921 ccaccaccga ctggtcgatg acgatcttgc acttgtcgat ggccttgccc acgctgccga
    13981 tgcgccatgc gtcgaggccg ttcatggttg cggccggcgt ggtttccgtg aggccgtaca
    14041 cgtcgtagag cggaatcccg atgtcccaga agaagtgtga gatctccacg ttcatcgcgg
    14101 cgctgccggt catggagccc ttaagacgtc cgccgagctt cgcgcgcacc ttgctgaaga
    14161 cgatcttgtc ggcgatggcg aacttgaagt tggcgccaaa gctcgattta ccctgcgccg
    14221 cgagttcgcg cttcttcttt ccgttttcga cgcccatgtc gaagagcttc ttcgccaggc
    14281 cgcccttctc gtttaccatg gtgatgaggc cgtcgtagac gcggttgaag acgcgcggca
    14341 cggcgatgag gaaggtgggc ttcaccttga cgatgtcgtc cacgatggtc gtggcgctct
    14401 ccgccaggcc catcgacgcg ccgatctgca tgaaggtaat catctcgccc aggccgtata
    14461 cgtgcgccca tggcaggatg ttgagcgtgc gctcgtcctc gtcgaagcgc gagaacatct
    14521 tgatgcgcgc gtgcgagttg ctggagaggt tcccctgcga cagcagtacg cccttcgggt
    14581 ctccggtggt ccccgaagtg tagatgagca cgccgatcac ggactggtcg ggtttgatgc
    14641 tcgcgacggg cttctccgcg cctttgcgct ccagctcctt caaggtgtcg gggccgtcgc
    14701 tctccatgag aatgaccttt tcgagcttgg gcgatgcgga gatgatgtcc ttgatcttgt
    14761 tcaggatctc cttcttcgat acgaggagca ccttgatgtc ggcgtcgttg atgatgtatt
    14821 tccatacctg gacgagttcc gcctcgtaca tcggcacgta gcgcgccgcg agcccgccgg
    14881 tcgcaaagaa gcccacggcc cagtcggtgc tgttgttgga gatgatcccg acggcgtcgt
    14941 ccttcttgat gccgagctgt gcaaggccgg cgcggcagtt gtcgacccgc ttcccgaagt
    15001 cggcgtaatt gatccattca tattcgccat ttttgttctt ttcgcccagg aacggccgct
    15061 gcgggtattt cgcgacggtg ttctcgagga actccacgac attgtccggt ttatccagct
    15121 ggtacatttt acctccctcg tgtgttggta cggtgatgat gtgtatggta atttattaga
    15181 atagtgttca atatttagaa tggcgttcgt ccatttcggt ccaccgggat tgactttgca
    15241 agttttttat ttgattggag gccgtatttt tccgcacttg tcgctggaca atgtctattt
    15301 atttgtcaga tgccggtcgc cggttgaagt gagtttttag aggtaataat cataccgaag
    15361 ctgttccccc aggtcgatca cccgcggcgg gacggccgcc ttttcgagca cggcgatcat
    15421 cttcgcgttg tgcgccagca cataggcgct catacgctcg atgccgcgct cgcggccgat
    15481 ttccagcaaa cggttgagca tgtagcgggc gatcccgcgc ccctggtagt gctcgtccac
    15541 gatgaaggcc atctcgtaga tgttctcctc ggcaaactgg gaatagcggc actcggcgat
    15601 gatccgcccc acgccgcgcg tctggatgat gcccaccagc gacagggtgg agatataatc
    15661 gacattgaga tactgttgca tctcggagtg gggcatggtg cgcagggcgg tgaagtagcg
    15721 caggtatttc gcctcgtccg aaaactggta gaagaggcgg cgcatcatgt cctcgtcgga
    15781 ggggcggatg gcgcgaaact tgaccgcaag gccgtccttg aacggtgcca tcgtctcgag
    15841 cgcgtagggg tagttgatgc cgtgctcgac cacgaagacc tggtcgccga agacgaggcc
    15901 gcccgccttg gcctcctcga agaggcggct gcgatgatcc gggtgcgcaa cctcgatgag
    15961 cgcaaggacg cgctcgcgga ttggcttggc gaagatggtg gcgacgccga actcggtcac
    16021 cacgtagcgg atggagccca gcatgcaccg gttctggatg gggctgccgg gctggctgat
    16081 gacgatgttg ctgtttccct gccggtcggt ggagcgcagc cccacgatca ccttgccccc
    16141 gcgcgagtgc gtggcggcca gcgcgaagtt gagcttgctg tcgaagccgg tgatctgcag
    16201 gtcgcgttcc gacaaaaaga cggagtcgcc ggtgatgtcg atcttgttca cgttgagcac
    16261 gctcaccagc ttcttgatcc ccagcatcgc ctgctggtgc gtggagttga acagcggcaa
    16321 cagccacacg ttcgggttgc cgttcacgaa gtcgtagagc cgccgcgtcc cgatgcacga
    16381 ggtggtgacg acggggctct ttcggcgcat ggcgtcgtag gaggcgagcg cgccggattc
    16441 gctgaggccg atgatccagt cggagacgat gtgcgtccac accttgaggt ttttcttctc
    16501 gcgcaggtgc gccgcgaccg cgtcgaagag ccgccccatg gagagcgaga cggtggagcc
    16561 gtcctcgacg agcgtggcga tctgccgccc gatgcagtcg agcgtgccgt cgtacgggcc
    16621 ggcggagaac tcgagcagcg gctcggcgct ctcgatcacg taatcgaatt cgctgagcga
    16681 catgcgggtg tcgccgtggg tgacgggcac gttcgggttg atctcggcga tggcgatcgc
    16741 ggcgttggcg atggcgatgc ggttgacgtc gtttacggtc ccgagagaga cgttcccgct
    16801 tttgtccggc gtcgaggttt ggacgatcgc gacgtcgatc tcgaccgccc cggagaggaa
    16861 catgaacggc agttccgcga tggtggtggg gacgatgtcg agcaggccca tctgcatcgc
    16921 gcgggcgctg ttctcgccga tgctgaaggt cttgaaccgg catttcgagg gggtgatggc
    16981 gcgcaggctc tcgaagtccg agggcgtggt gagctggatc acctcgatgt cctgcaggtt
    17041 cgggtggtcg gtggccgcca ggtgccggat ggtgtgcacc ggcgtcgccg ggccgccgct
    17101 caggaagagc cggtagccgg gcttgatgat ctcgcagagc ccttcgacag tgatgcggcg
    17161 gtcccgctcg gcggccttct gcgcgcgcgt tttgaggttg agaaaaccca tggccgcaac
    17221 gatgcgcacg gtgcgaaaaa aatatcaaca actaaatatg catgatggcg ccggtgcggc
    17281 ctggcgctgg gaccatgaaa aaactattga aataatcgcc tccccggatt ttgttgacca
    17341 gcggtatcca aacggaccca tgcggggggc gcgaccatga ttcagaatgc gaattttctg
    17401 tgcgagatcg gctgcgagga ggtccccgcc ggctatgtcc cgccggcgtg tgcggccatt
    17461 cgcgcgctct tcgagaaaag attggccgat gcgcgcatcg cctacaccga actgggcgtg
    17521 tacgccacgc cccggcgctt cgccgtgctg gccagcggta tggccgagcg gcaggccgag
    17581 gaggaggttg aggtcaaggg gccgtcggtc aaggccgcct acgacgcctc cggcgcgccc
    17641 acgaaggcgc tcgaggggtt cctcaaggga aacgccctcg ccctcgccga tgtgtttccg
    17701 aaggcaacgg agaagggcga gtatctcttt gcgcgcaaga cggtcgcggc ggtcgacacc
    17761 gcagcggtgc tgcccgcgat cgtaaacgac ctcgtcgcga ccgtcccctt tcccaagcgg
    17821 atgcgctgga gcgacaaggc cgtcaccttc ccgcgcccgg tccgctacgt gctgctcatg
    17881 ctcaatgacc gcgtggtgcc cttcgagatt tcgggcatcg ccgcggggaa gagcacccgg
    17941 gggcattacg tccagcacaa ccgcatggtc gagctttcgt ccatcgccgc ctacgagacg
    18001 acgctcgagg agcacgccgt gatcgtcgat caggagcgcc ggcgcgccat gatcgcggac
    18061 gaactcgcgg ccgccgcgaa gcggcttcac ggcgtcctgg tggaggacga ggacctcctg
    18121 gacacggtga cctatctcgt ggagcggcc
//
LOCUS       DAORHF010000045        18111 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2202_length_18111_cov_5.144218, whole genome shotgun sequence.
ACCESSION   DAORHF010000045 DAORHF010000000
VERSION     DAORHF010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 18111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18111
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2202_length_18111_cov_5.144218"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..1372
                     /gene="leuC"
                     /locus_tag="PKJ16_05905"
     CDS             113..1372
                     /gene="leuC"
                     /locus_tag="PKJ16_05905"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HNV46557.1"
                     /translation="MGMTITEKILAAHAGVGRVEPGELIDAKIDLALGNDITAPIAIR
                     EFRALGVKGVFDREKIALVADHFTPNKDIMSAEQVKILRDFSREQGIVHFYDTGRVGV
                     EHALLPELGLARPGMLIIGADSHTCTYGGLGAFSTGVGSTDLAAVMATGSLWFKVPES
                     MKFIFRGKLGRYVTAKDLILFTIGDIGVDGALYRAMEFAGDTIDALTVEGRLTMANMA
                     IEAGGKNGIFAADEKALAYVQSRTSLPFTVFASDPDAAYVDVREYDATKIEPLVSAPH
                     LPENTKPARENGTTIDQVVIGSCTNGRIEDLRLAAEVLKGKTAHRDVRLIIIPATQEV
                     YQTAMKEGLFDVFLAAEAAISAPTCGPCLGGHMGILAEGERCVATTNRNFVGRMGHPR
                     SEVYLASPAVAAASAVLGRIGHPDELT"
     gene            1436..1924
                     /gene="leuD"
                     /locus_tag="PKJ16_05910"
     CDS             1436..1924
                     /gene="leuD"
                     /locus_tag="PKJ16_05910"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HNV46558.1"
                     /translation="MKAKGKTWKFGDDINTDEIIPARYLNTSDPNELARHCMEDADPD
                     FMKKAAPGDIIVAGSNFGCGSSREHAPIALKAAKISCVIARSFARIFYRNSINIGFPI
                     VESPEAADDIRPGDELEVDFDNGVIRNATSGKEYAAKPFPPFMKEIIEKGGLMNTIKS
                     KR"
     gene            complement(1921..3993)
                     /locus_tag="PKJ16_05915"
     CDS             complement(1921..3993)
                     /locus_tag="PKJ16_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46559.1"
                     /translation="MKPRKILKPLLLCSALLVLCAAAGAAFFLYRYPKERLGARLAAQ
                     IGDAIKRRVVVQSVEYGLNGVILRNVTIRDGMRDSDPVLASVEEARLRFSIRSLVLSL
                     EFKVWWVLLQNAFVVLSTDGERWNIERLIDDIGGGSKSPMTASVSRVILSGCRFALAE
                     TPGPLRPLAGTYGIGGVIDIEPGAPITIRDARVRLPEGRGSCAGDLAVRREERGATLT
                     GDVRLDNCSLGWVYRWRRRGPLPFAAVSGSITGLSVSTRAVSGAVNADVVADTGARIA
                     VRGRCAVDIAGRRVAITQAVGTTGSSRVALEEAAFTFGGDLNRLRLGDIDADAREINR
                     VYPLLPERIAGRVTGRLSRERGRLSGSLVLGDAAYNPEEQTVTGVNGAIVIENNVFTV
                     RDLPVRFRGQPLRVSVASTDGNLNRLFLDISAPEFRINDMPSGDSRGPSFSGGLPFVV
                     TGKSAIGTVFWDNLAVTGFAVSYGLEKDVLSVHHYSCRLFGGEVSGRGTVRFGRTERT
                     VDVSVAFKNAKLQELARLSDKTRDRMFGIASGVAGLQITLPERTGARPEIRGTIVFSI
                     VKGKFVDTGIQNGLVAILKELQYKLRDLEFERISGTIVVQGDRFVINSLTLQAPEIVV
                     TFGGYATRSLAGDLRIELDFSRAFLKDVPNPAVWGISKYRREGRYRIPVVAKGGNITS
                     STNIKLAD"
     gene            complement(3971..5191)
                     /locus_tag="PKJ16_05920"
     CDS             complement(3971..5191)
                     /locus_tag="PKJ16_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV46560.1"
                     /translation="MASAQFKVENYLAKSFITVEGKKNSDNFYIIRTGKVKLQKENPV
                     VAEDPYSVLGPGDFFAVISCMSGHSRIETAVALTDVSLISVERDQFGILIQRNPVVAM
                     KIIRFFSKQLREFDQAITRLTFKNIVEEEPSHLYNIGEFYFTRKSFNHAAYAFQRYLQ
                     YCPNGDYKNKAVERLKALRAPLRVPENPQASSLTRNYRDNVMVFCENEPGQELYIIQA
                     GKVKITKIVDDEVLLAVLKAGDIFGEMALLDNKPRSASAISFGDVTLFAINKANFEGM
                     VQAQPQMATRLIQLLSERIWTAYRQLENIMIKDPIGRIYDTLLIQVEKQKIRIASKES
                     HNFEIGTKELVNMVGLPPDKGEQLIVQLLENKHFRLEAGKIICTDLAELEKTVQFYRK
                     KTVMEKKREASKNP"
     gene            5444..6328
                     /locus_tag="PKJ16_05925"
     CDS             5444..6328
                     /locus_tag="PKJ16_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HNV46561.1"
                     /translation="MPASGWTKTLVCFSMLIALAALANCSRAQFTEVEPNDTPRQATQ
                     ITVNATVRGVIGTAGDTDFFAFAIARPTVLDIRLSAAEGIDHGFTIGAIDHAGFTPLK
                     TVDDGRHGAPERMCNFHAAPGGYVVMVRHRDAIGAATRGRPYHLVIDGRPARPNEERE
                     PNDAPSAANPIVAGQEMRGYFSPGRNPENRRPTAEFREEDWYSIDVPADGASEASLID
                     ITLIGAPSINALVCLHNRSGAVLGCADKNGVGGDESLMDAPLESAGRYYIMVAALDNA
                     ANGNIPYLLTVRVRPRSR"
     gene            complement(6384..9074)
                     /locus_tag="PKJ16_05930"
     CDS             complement(6384..9074)
                     /locus_tag="PKJ16_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46562.1"
                     /translation="MNSAAQLKRILIVEDEPIVGLDLQAMLEPMGYAVMAIVPTADEA
                     YRAALAGSPDLILMDVRLKEGSDGVDAARRIRRELDAPIIYITATADDATVDRARDTM
                     PYGYLIKPITERDLVTNVDSAITRHAMERALRESEERFRTIFEAANDSIIIHDIDTGG
                     IVDANRRAIEFYGGEDLNALWSDDRWADPPYSAADALRWIRRAADEGPQTFEWKYLHH
                     SGECFWEEVSLTKIRYGGAERVLAVSRNITERKRMEAALRESEQLYRTLVDTSPDAII
                     MSDLAGNILFANMQAARLLGYGSVEEYILGMKNVFMPIAPDDRARAIENAQRTLETGA
                     VSGIEYTILRRNGEAFPAELSASLVYDADHRPRGFIAVVRDISERKRAEESLREMMGE
                     LRAIYDSQHDGIYVVDSETRRFMRVNSSLCEHFGYSAAEFCSLIVEDVHPPDALPRVR
                     EEFAMLRDGLKDIAPAIPCLRKDGSVFFADIAARRIVFHGRPALTGSFRDITERIRAE
                     DEKRRLEEQLRQAQKMESIGRLAGGVAHDFNNLLTVIMGNVSLALMDLNASNPLYKTL
                     DVVMRAVESAASLTRQLLAFSRKQIIEPRSINMSELVQDIRDILGRVAGDAARLELRL
                     APESGFVQADRGQLEQMIINLVSNARDAMPEGGTIRIETADVDLDAEYCRRHPEATPG
                     RHIVLSVIDTGIGMSPEILANIFEPFFTTKPHGKGTGLGLSTVYGAVKQNGGSIEVDS
                     AKGRGTTFRIFLPRLAEPQLGPIAPEAETDLPTGTETIMLVEDEPVVRDLTLNLLMRQ
                     GYRVLHYAGAGEALIALEGYADPIHLLLTDIIMPGLNGRELARMVLEKRPETKVLLIS
                     GYSGSSSLAGDVHEAFNFLPKPYTPSVLARKIREILDSKP"
     gene            complement(9218..11044)
                     /locus_tag="PKJ16_05935"
     CDS             complement(9218..11044)
                     /locus_tag="PKJ16_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46563.1"
                     /translation="MYIVLIVTLVAVLGIVIWALFGKGEGLTDKDDVESPDRVFRRRA
                     TDQEIEKRFPDQPDRRARQDPAERTGPSRKSDSEAGGQEVDDDFELPYKADEIISDSS
                     RFRIYKRTLINSEIYARNSDFATAISLYEGVNSRINDENTNRKIEANIEYLKKFQETQ
                     DIRRTAKDALSRKGKAKKTSEIKLTFDGPVSLPENLNIGLVPPPRPEDRQDIDVAKIV
                     DEVTRKIEEKGLPAGGADLKRFSEDIDHLRGSLNELKEVRDTLRSEVAGDVRHNTDHA
                     IAGELAALRSKIENLAGEKERLRQELESMKVGRDSGAGDAELITQGLDRLSETSGQLS
                     QTMRSLEEIARMNAASAASAVADAANLPATTEAKYTAAEDLAKLEDLLSKVPRIDAPD
                     PRRLDARPRKGRDTRFPDEAKGDLHKRTEALEKIEKRREDETEEIELLSDYIEDKPHD
                     TMSDEEIFEKILQDRAPGDHSAFEIIGDRGRDAELPYDATIDEMQRHQQDEMKFYEKF
                     LKHDKRKRKELPILKVSYDFSRLPDEFSLARDKNILEYSFYKYKGMLEKANDFIKKRK
                     VRDAINYYKVVMGQNIPPEFKSMIRKNINDLTEYLEKYLTAD"
     gene            complement(11112..11570)
                     /locus_tag="PKJ16_05940"
     CDS             complement(11112..11570)
                     /locus_tag="PKJ16_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46564.1"
                     /translation="MPIDTTLYVHESTLDSICEICALTGRTRNRIIVMLLRAALRDRS
                     RAVRIGCSVRYQSRDRREKWRTVHVRLSEDEADYFKDLRNFYRMSDSLILAIAVRDYF
                     DDILRQSRSGGMTDNYPFSNYILSREEIDGLVFWRICWGFPEKPEKYFLL"
     gene            complement(11662..13800)
                     /gene="glgX"
                     /locus_tag="PKJ16_05945"
     CDS             complement(11662..13800)
                     /gene="glgX"
                     /locus_tag="PKJ16_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNV46565.1"
                     /translation="MKPGRITLPGYPSPLGVKLDMNGAQFAVFSRHASSVTLVLFESA
                     DPDAPVEEFVLDPHLNKTGDIWHLWIAGVSEGQLYGYRMDGPYLPFRGHRFNPHKLLL
                     DPYARSIAGGPVWDLRHSRGFDPDSPEADLSFSKADNAACAPRCIVVNTEVDLFDRPL
                     KTPLADTIIYELHVRGFSVHPSSGVEFAGTYRGIIEKIPYLKELGVTAVELMPVQEFD
                     ELENINVDPATGERLRNYWGYSTMSFFAPRGRYSHAGCMGEQVYEFKDMMCALHEAGL
                     EVILDVVFNHTAEGDQMGPTLSFRGIDNSIYYILDENRRLYKNFSGCGNTINCNHPLV
                     RDFIIDCLKFWVVEMHVDGFRFDLASILGRDQTGAILANPPLIEKIMEDPILRSTKII
                     AEAWDAAGAYQVGNFPGRWAEWNGKFRDDVRRFWRGDENTVGYFATRITGSSDLYGGE
                     NKGPLHSINFITCHDGFTMNDLVSFRDKHNEANGEGNRDGENTNLSANYGIEGAEVTP
                     FIERRRVRQIKNFIATLFLAQGVPMLLAGDEFRRTQRGNNNAYCQDNDISWIDWGCLE
                     RHREIVRFCREMIRFRKEHHALRKNEFFTGSAHNDHPEPDISWHGTRPGEPDWLPGSR
                     FIACLLNGAYVEDEYGTPDVDIYLAFNASLFSYPVLLPPPPSGGAWRVAVDTALPEPD
                     DIAPSGCERPLDGLRYRVQRGSTVVLISGA"
     gene            complement(13825..14175)
                     /locus_tag="PKJ16_05950"
     CDS             complement(13825..14175)
                     /locus_tag="PKJ16_05950"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(14005..14007),aa:Sec)
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNV46566.1"
                     /translation="MLSAVVTLVSCDDPGVVFEFETFDFGAVEQEKSLQAVFAFRNAG
                     KRTLVIERVQPTUGCAAALLSSKEIPAGGEGKIEVTLYTNKYTGTMSKTIQVFTNDPE
                     RPKISLRVHATVRE"
     gene            complement(14274..15836)
                     /locus_tag="PKJ16_05955"
     CDS             complement(14274..15836)
                     /locus_tag="PKJ16_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV46567.1"
                     /translation="MTLARWLIFIGVLGIIVMSLGFTSLRSDMLERDYTESLVDISNR
                     IALAVNRVFKGADPRRPEAEMPTAVVAISAKYRPELIAVFDRENRVIADKKLYQAIAA
                     DELYDSIINEFREGALAPDPGRQFVVRYYNRAKCYLFVKSVTEGRILIIFPYRMTAAA
                     KVRLGLELALLCMGVVLVLTVVYILLAKRKPVTEEPTEESEPYRDVTVDRKRLAKNRE
                     NSPIYRDMRNAAAASLDAHVYEGFSAIAARTTPDAVSLYIARDRASRLEKRYELNGQA
                     FLKVDSGSYEAIDVDNEIGEGLRNESLLMLDGGRRVIAPVLYRDALMGAVVILRQLPF
                     SGAEIHDIRDEIVAMARFINEYMLLNDVMVDRDTGVYSRAFLKLKYDELLNQPRGARV
                     PLAVMAVKAINGTPEEGSEERRLLLQAVAERIYEHLRADDVLSAGDDFVVLMKGAGAR
                     EAAAAEEGVLRALSNVRITIDKKSYPLRPFTGIASIEEGSADPLEDARRNLERAVAEA
                     TINADGRTEPGA"
     gene            16271..16636
                     /locus_tag="PKJ16_05960"
     CDS             16271..16636
                     /locus_tag="PKJ16_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46568.1"
                     /translation="MKIYGFSTSSKLNDRRPHEHHVWGSSALKRERSDTDSVVISPEA
                     RERFAAARAIDMEHARSRRLARELAPAIRRAIDAGGETDDARADRIEALRRAFATGDA
                     RMSDTALRDTADALLALAM"
     gene            complement(16649..17152)
                     /locus_tag="PKJ16_05965"
     CDS             complement(16649..17152)
                     /locus_tag="PKJ16_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46569.1"
                     /translation="MKRGYRFSRTSRYACRAIVAVLLWSFGPSATVFPTTDESVSNTR
                     GFLAGLGGGYLVPGGTFQKDLNPGIGYGATLLYFSAWNMILFESNLLYGRYDVNFSPG
                     SYLSQVTVTVGPGLYFPVFDWLQPYVSLQGGVNYLSFTFSQTGAREDAYKPIGLMSAG
                     LLVQPFD"
     gene            17978..>18111
                     /locus_tag="PKJ16_05970"
     CDS             17978..>18111
                     /locus_tag="PKJ16_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV46570.1"
                     /translation="MIINHNMSAINANRSLKFTSWEVGKSMQKLASGLRINKAGDDAS
                     G"
BASE COUNT         3475 a         5730 c         5446 g         3460 t
ORIGIN      
        1 cgaatattgc cgcgcggaaa aggcgcttga tttttttacc gccgatttcc atggtgcaca
       61 gattccgttt ccggcaggca gccggacgac cctgttcgat ggagcgcaca tcatgggaat
      121 gaccatcacc gaaaagatcc tcgccgcgca tgcgggcgtc ggccgcgtcg agccgggcga
      181 gctcatcgat gcgaaaatcg acctcgcgct gggcaacgac atcaccgcgc ccatcgccat
      241 cagggagttc cgcgcgctcg gcgtgaaggg cgtattcgac cgcgagaaga tcgcgctggt
      301 ggccgaccac ttcacaccca acaaggacat catgtccgcc gagcaggtga agatcctgcg
      361 cgacttctcg cgcgagcagg gcatcgtgca cttctacgac accggacgcg tgggcgtcga
      421 gcacgcgctc ttgcccgaac tcgggcttgc gcgcccgggg atgctcatca tcggcgccga
      481 ctcgcacacc tgcacctacg gcgggctcgg ggcattctcc accggcgtgg gctccaccga
      541 cctcgctgcc gtcatggcca ccgggagcct ctggttcaag gtgcccgagt caatgaaatt
      601 catctttcgc gggaagctcg gacgttacgt gaccgcgaaa gacctcatcc ttttcaccat
      661 cggcgacatc ggggttgatg gagcgctgta ccgggcgatg gagttcgccg gcgacaccat
      721 cgacgcgctc acggtcgagg gacgcctcac catggccaac atggccatcg aggccggcgg
      781 caagaacggc atcttcgccg ccgacgaaaa ggcgctcgca tatgtacaga gcaggacgtc
      841 ccttcccttc acggtctttg cgagcgatcc cgatgccgcg tatgtcgatg ttcgcgaata
      901 cgatgcgacg aagatcgaac ccctggtgtc cgcgccccat ctgcccgaga acacgaagcc
      961 cgcgcgggag aacggcacga cgatcgacca ggtggtcatc ggatcatgca ccaacgggcg
     1021 catcgaagac ctgcgcctcg cggcggaggt gctcaagggg aaaacggcgc accgggacgt
     1081 gcggctcatc atcatccccg ccacccagga ggtgtaccag acggcgatga aggagggcct
     1141 gttcgacgta ttcctcgcgg cggaggcggc catatcggct cccacctgcg gaccgtgcct
     1201 gggcgggcac atgggcatcc tcgccgaggg cgagcgctgc gtcgcgacca ccaaccgaaa
     1261 cttcgtcggc cgcatgggac atcccaggag cgaggtctat ctcgccagcc ccgcggtcgc
     1321 cgctgcatcg gcggtgctgg gcaggatcgg ccacccggat gaactgacgt agggtacggg
     1381 cagggcaccg ccctgcccct cctgtgcact ggcgctatca ccacggagga aacgcatgaa
     1441 ggccaaagga aaaacctgga aattcgggga tgacatcaac accgacgaga tcattccggc
     1501 gcgttacctg aacacgtccg acccgaacga gctcgcacgg cactgcatgg aggacgccga
     1561 tcccgacttc atgaagaagg ccgcgccggg cgacatcatc gtggccgggt ccaacttcgg
     1621 ctgcggctcc tcgcgggagc acgcgcccat cgccctcaag gccgcgaaga tctcctgcgt
     1681 gatcgcacgg tccttcgcgc gcatcttcta ccgcaacagc atcaacatcg gcttccccat
     1741 cgtggaatcg cccgaggccg cggacgacat ccggcccggc gacgagctcg aggtggactt
     1801 cgacaatggc gttatccgca acgccacctc cggcaaggaa tacgcggcga agcccttccc
     1861 gccgttcatg aaggagatca tcgaaaaggg cgggctgatg aacacgatca aaagcaagcg
     1921 ctagtcggcc agtttgatgt tcgtgctgct ggtgatgttc ccgcccttgg ccacaaccgg
     1981 tatgcggtag cggccctcgc gccgatactt gctgatgccc caaaccgccg gattggggac
     2041 gtccttgaga aaggcgcggc tgaaatccag ctcgatgcga aggtcgcccg cgaggcttcg
     2101 ggtggcgtac ccgccgaagg tcaccacgat ctccggcgcc tgcagggtga ggctgttgat
     2161 gacgaagcga tcgccctgaa cgacgatggt gccgctgatc ctctcgaact cgagatcccg
     2221 cagcttgtac tgcagttcct tcagaatggc gaccaggccg ttctggatcc cggtatcgac
     2281 aaacttaccc ttgacgatcg agaagacgat ggtaccccgg atctccggcc gcgcccccgt
     2341 ccgttcgggg agcgttatct gcaaccccgc gaccccgctc gcgattccga acatgcggtc
     2401 ccgtgtcttg tcgctcaggc gcgccagctc ctgcagcttc gcgttcttga acgcgacgga
     2461 cacgtcgacc gtccgttccg ttctcccgaa gcggacggtc ccgcggccgc tcacctctcc
     2521 gccgaacaga cggcatgagt aatgatgcac cgacagcacg tccttctcga ggccatacga
     2581 cacggcaaac cccgtgaccg ccaggttgtc ccagaacacg gtcccgatgg cggactttcc
     2641 ggtaacgacg aacgggaggc ccccggaaaa cgacggcccg cgggagtcgc ccgacggcat
     2701 gtcgttgatg cgaaactcgg gcgcggagat atcgaggaaa agcctgttga gatttccatc
     2761 ggtggacgcg acggacacgc gcaggggttg accgcgaaac cggaccggca ggtcgcgaac
     2821 ggtaaagacg ttgttctcga tgacgatcgc gccgttcacg ccggtgacgg tttgctcctc
     2881 gggattgtat gccgcgtcgc cgagaacaag cgagccgctc aggcgtccgc gttcgcggga
     2941 caggcgtccg gtcacgcgcc cggcgatacg ctccgggaga agcggataga cgcggttgat
     3001 ctcgcgcgcg tcggcgtcga tgtcgcccag ccgcaggcgg ttcaggtcgc cgccgaaggt
     3061 gaaggccgcc tcctcgagcg ccacgcgcga cgaaccggtc gtgccgacgg cctgcgtgat
     3121 ggcgacgcgg cgacccgcga tgtccacggc gcaccgcccg cggacggcga tgcgcgcgcc
     3181 ggtatcggcc acaacgtccg cattcaccgc accgctcacc gcgcgcgtgc tcaccgagag
     3241 acccgtgatt gatccggaaa ccgcggcgaa gggaagcggg ccgcgccggc gccagcggta
     3301 cacccatccc agggagcagt tgtcgagccg cacatcgccc gtaagcgtcg cgccgcgctc
     3361 ctcccgcctg acggcaagat cgccggcgca cgacccccgc ccctccggga gccgcacgcg
     3421 cgcatcgcgt atcgtaatcg gcgcgccggg ctcgatgtcg atcacgccgc cgatcccgta
     3481 ggtgccggcg agcgggcgca gcggtcccgg cgtctccgca agcgcaaagc ggcaacccga
     3541 caggatcacg cgcgacacgg atgccgtcat cggggatttc gaaccgccgc cgatatcgtc
     3601 gatgaggcgc tcgatattcc agcgttcccc gtcggtcgaa agcaccacaa aggcgttctg
     3661 cagaaggacc caccaaacct tgaactcgag cgagagcacc agggagcgga tcgaaaaacg
     3721 aaggcgcgcc tcctccacgg aggcgaggac cgggtcgctg tcgcgcatcc cgtcgcggat
     3781 ggttacgttc ctgaggatca cgccattgag gccgtactcg accgactgga ccaccacccg
     3841 gcgcttgatg gcgtcgccga tctgcgcggc caggcgagcg cccaatcgct ccttggggta
     3901 tcggtataaa aagaaagccg caccggcggc cgcacaaaga acgaggagag cgctacacag
     3961 gagaagcggc ttaaggattt ttcgaggctt cacgcttctt ttccatgacc gtctttttgc
     4021 gatagaactg cacggtcttc tcgagctcgg ccaggtcggt gcagatgatc ttcccggctt
     4081 cgagccggaa gtgcttgttc tcgaggagct ggacgatgag ctgctcgccc ttgtccggcg
     4141 gcagccccac catgttcacg agctccttgg tcccgatctc gaaattatgc gactccttgc
     4201 tcgcgatcct gatcttctgc ttctcgacct gaatgagcag cgtgtcgtag atgcggccga
     4261 tggggtcctt gatcatgatg ttctcgagct gccggtaggc cgtccagatc cgctcggaga
     4321 ggagctggat gaggcgcgtg gccatctgcg gctgcgcctg caccatgccc tcgaagttcg
     4381 ccttgttgat cgcgaacagc gtcacgtcgc cgaagctgat ggccgaggcg cttctgggct
     4441 tattgtccag gagcgccatc tcgccgaaga tgtcgcccgc cttgagcacg gcgagcagca
     4501 cctcgtcgtc gacgatcttg gttatcttga ccttgcccgc ctggatgatg tagagctcct
     4561 ggccgggttc gttctcgcag aagaccatca cgttgtcgcg gtagttccgg gtgaggctgc
     4621 tggcctgggg gttttccgga acgcgcagcg gcgcgcgcaa cgccttgagc cgctccacgg
     4681 ccttgttctt ataatcgccg ttgggacagt actgcagata gcgctggaag gcgtaggcgg
     4741 cgtgattgaa gctcttgcgc gtgaagtaaa actcgccgat gttgtacagg tgcgacggct
     4801 cctcctccac gatgttcttg aaggtgagcc gggtgatcgc ctggtcgaat tcgcgcagct
     4861 gcttgctgaa gaagcggatg atcttcatgg ccacgacggg attgcgctgg atgaggatgc
     4921 cgaactggtc gcgctcgacg gagatgagtg agacgtcggt gagcgccacc gcggtctcga
     4981 tgcgcgaatg cccgctcatg caggagatca cggcgaaaaa gtcccccggg cccagcacgc
     5041 tgtacgggtc ctccgccacg accgggttct ccttctggag cttcaccttc ccggtgcgaa
     5101 tgatgtaaaa attgtccgag ttcttcttcc cctccaccgt gatgaaggat ttggcaaggt
     5161 agttctcgac tttaaactgg gcagacgcca ttgctcctcg ctcggtgaaa cggttgcgcg
     5221 cgacgatcgg aagacgcgaa cgacgcaccc gacgcggcac cgtctgcgcg ccggggaggt
     5281 tctacagtca gaatcgtcat aaaaacggct aatttcaaga agaattttaa ccctcaaggg
     5341 ccgatttgtg cttgtgctgc gccccttcgc gggcgcatgt ttccgcatgc ggcggcgcgc
     5401 ccggcgccgc catcatccca cactgccgga atggaggcgg tacatgccag catcgggttg
     5461 gacaaagacc ctcgtttgct tttctatgtt gattgcgctc gcggcgctcg cgaactgctc
     5521 gcgcgcgcaa ttcaccgagg tcgaaccgaa cgatacgccg cggcaggcca cgcagataac
     5581 ggtgaacgcg accgtccgcg gcgtcatcgg gaccgcgggc gacacggact ttttcgcctt
     5641 tgcgatagcg cgcccaaccg tcctcgacat acggctctcg gccgccgaag ggatcgacca
     5701 tggctttacg atcggggcca tcgaccacgc gggattcacg ccgctcaaga cggtcgatga
     5761 cggccgtcac ggggcgccgg agcgcatgtg caacttccac gccgctccgg gcggttacgt
     5821 cgtgatggtg cgtcaccggg atgcgatcgg cgcggccacc cgcgggaggc cgtaccatct
     5881 cgtcatcgat ggccgccccg cgcgccccaa cgaggagcgc gagccaaacg acgcgccgtc
     5941 cgcggcgaat cccattgtcg cgggacagga gatgcgcggt tacttttcgc cggggcgcaa
     6001 tcccgagaac cggcgcccca cggccgaatt cagggaggag gactggtatt ccatagatgt
     6061 ccctgcggac ggtgcaagcg aggcttcgct catcgacatc accctgatcg gggcgccgtc
     6121 gataaacgcc ctcgtatgcc tccataaccg aagcggtgcg gtcctgggtt gcgccgataa
     6181 aaatggcgtt gggggcgacg aatcgctcat ggacgccccc ctggaatccg cgggcaggta
     6241 ttatatcatg gtggccgcgc tggacaatgc ggcgaacgga aatatcccct atctgctcac
     6301 ggtgcgcgtc cgaccgcgct cgcgataggt tcatagcggg ggtgacggga gcatgggaga
     6361 tggcgccgat caacgaacaa cggtcacggt ttcgagtcga gtatctccct gattttccga
     6421 gcaagcacgg acggcgtata cggtttcggc aggaaattga acgcctcgtg gacatcgccc
     6481 gcaagcgagg agcttccgga atagcctgat atgagaagca ccttcgtctc agggcgtttt
     6541 tcaagcacca tgcgcgccag ctcgcgccca ttgagacccg gcatgatgat atccgtgagc
     6601 aggagatgga tcgggtcggc gtatccctcc agggcgataa gcgcctcgcc ggccccggcg
     6661 taatgcagca cgcggtagcc ctgccgcata agcagattga gcgtgagatc gcgcaccacc
     6721 ggctcgtcct cgaccagcat gatggtttcg gtcccggtgg gaaggtccgt ttcggcctcc
     6781 ggcgcgatcg gcccaagctg cggctcggca agacgcggga gaaagatgcg aaaggtggtc
     6841 ccccggccct tcgcggaatc gacctcaatc gatcccccgt tctgcttcac tgccccgtat
     6901 acggtcgaga gaccgagccc ggtccccttg ccgtgcggct tggtggtaaa gaagggctcg
     6961 aagatattgg cgagtatctc cggggacatc ccgatcccgg tatcgatcac cgacagcacg
     7021 atatgccggc cgggggtcgc ctcgggatgg cggcggcaat actcggcatc cagatcgaca
     7081 tcggccgttt caatgcggat cgtcccgccc tcgggcatcg catcgcgcgc attggacacc
     7141 aggttgatga tcatctgctc caattgaccg cgatcggcct ggacgaatcc cgactcgggc
     7201 gcgagccgca gctccagccg cgcggcatcg ccggccaccc ggcccaggat gtcgcgaatg
     7261 tcctgcacga gctcgctcat attgatgctg cgcggttcga tgatctgctt gcggctgaag
     7321 gcgagaagct ggcgggtaag cgacgccgcg ctctccacgg cgcgcatcac cacatcgagc
     7381 gtcttgtaca gcgggttcga ggcattcagg tccatcagcg cgagcgacac gttgcccatg
     7441 atcacggtga gcagattgtt aaagtcgtgc gccacgccgc ccgcgagccg gccgatcgat
     7501 tccatcttct gcgcctggcg aagttgctcc tcgagacggc gcttttcgtc ttcggcgcgg
     7561 atgcgctcgg tgatgtcccg gaatgatccc gtgagggccg gacggccatg gaagacgatc
     7621 cggcgcgccg cgatatcggc gaagaacacg ctgccgtcct tccggaggca gggaatggcc
     7681 ggcgcgatgt ccttcaggcc gtcccgaagc atggcaaact cctcgcggac gcgcgggagc
     7741 gcgtccggcg ggtggacatc ctccacgatc agcgagcaaa actccgcggc gctgtaaccg
     7801 aaatgctcgc acagcgacga gttaacccgc atgaaacgcc gggtctcgct gtcgaccacg
     7861 taaatcccgt cgtgctggct gtcatagatc gcgcggagct cgcccatcat ctcccgtagg
     7921 gactcctccg cccgcttccg ctccgagata tcgcgcacaa cggcaatgaa gccacgcgga
     7981 cggtgatcgg cgtcgtacac cagcgaggcg ctcaactccg cgggaaaggc ctcgccgttt
     8041 cggcggagga tcgtgtactc gatgccgctc acggcgccgg tctcgagcgt tcgctgcgcg
     8101 ttctcgatgg cgcgggcccg gtcgtccgga gcgatgggca tgaagacgtt cttcatgccc
     8161 aggatgtact cctcgaccga accgtagccg agaagccgcg ccgcctgcat gttcgcaaac
     8221 agaatgttcc ccgccaggtc gctcataatg atggcgtcgg gggaggtgtc cacgagcgtc
     8281 cggtaaagct gctcgctttc gcgcagcgcg gcctccatcc gcttccgctc ggtgatgttc
     8341 cgcgagacgg ccagcacccg ctccgcgccc ccgtaccgta ttttcgtgag actcacctct
     8401 tcccaaaagc actcgccaga atggtgcaga tacttccact cgaaggtttg cggaccctcg
     8461 tcggccgcgc ggcgaatcca gcgcaacgca tctgcggccg aatacggcgg gtcggcccag
     8521 cggtcgtccg accaaagcgc atttaagtcc tcgccgccgt agaactcaat ggctcgacgg
     8581 ttggcatcca cgattccgcc ggtatcaata tcatggataa taatggaatc gttcgccgct
     8641 tcgaagatgg tccggaaacg ctcctcgctc tcgcgcagcg cgcgttccat ggcgtgtcgc
     8701 gtgatggccg aatcgacatt ggtgacgagg tcccgctcgg tgatgggctt gataagatag
     8761 ccgtacggca tggtatcgcg cgcacggtcg acggttgcat cgtccgcggt cgcggtgatg
     8821 tagatgatcg gcgcgtcgag ctcgcgcctg atgcgccgcg cggcatcgac cccgtcactg
     8881 ccctccttga ggcgaacgtc catcaggata aggtcgggcg atccggcgag ggccgcgcga
     8941 tacgcctcgt ccgcggtcgg cacgatggcc atcaccgcat accccatggg ctcgagcatg
     9001 gcctgcaggt cgagccccac gatgggctcg tcctcgacga tcaggattcg tttgagttgc
     9061 gcagccgaat tcatgccatg ccattggcgc accgcccgaa ccacgcgtga tcgaacgatg
     9121 cgaattgtat gtgtggatca tcccgctaaa ttgaactgat cataccggcc gtgtgatatc
     9181 tgtcaagccc tttggggggc gccgggccgc tcgccgatca gtcggccgtg aggtactttt
     9241 cgaggtattc ggtcaggtcg ttgatgttct ttcggatcat ggacttgaac tcgggcggaa
     9301 tgttttgccc catcacgacc ttgtagtaat tgatggcgtc gcgcaccttg cgcttcttga
     9361 taaagtcgtt cgctttttcc agcatcccct tgtatttata aaaagaatac tcgaggatgt
     9421 tcttgtccct cgcgaggcta aactcgtccg gcaaccttga aaaatcgtaa ctcaccttca
     9481 ggatgggcag ctccttgcgc tttcgcttgt cgtgcttgag gaacttctcg taaaacttca
     9541 tctcgtcctg ctggtgccgc tgcatctcgt cgatggtcgc atcgtaggga agctcggcgt
     9601 cacgcccgcg atcgccgatg atctcgaacg cgctgtgatc gcccggcgcg cggtcctgca
     9661 ggatcttctc gaatatctcc tcgtcgctca tcgtatcgtg gggcttgtct tcgatgtagt
     9721 cgctgaggag ctcgatttcc tcggtctcgt cctcgcgacg cttctcgatc ttctcgagcg
     9781 cctcggttcg cttgtgcagg tctcccttcg cctcgtcggg aaaacgggta tcgcggccct
     9841 ttcgcgggcg cgcatcgagc cgtcgcgggt ccggcgcatc gatgcgggga accttcgaca
     9901 ataaatcctc gagcttcgcc aggtcctccg cggcggtgta tttcgcctcc gtcgtggcgg
     9961 gaagattcgc cgcgtccgcc accgccgatg cggcggacgc cgcgttcatc cgcgcgatct
    10021 cctcgaggct gcgcatcgtt tggctcagct gtccgctggt ttccgacagc ctgtcgaggc
    10081 cctgcgtgat aagctccgca tccccggcgc cggaatcgcg cccgactttc atcgactcga
    10141 gctcctgtcg caaacgctcc ttttcgccgg cgaggttctc gatcttggaa cgcagcgccg
    10201 caagctctcc ggcgatggcg tgatccgtat tgtgccgaac atcgcccgcg acctcggagc
    10261 ggagggtgtc gcgcacctcc ttgagctcgt tcaggctgcc gcgcaggtgg tcgatgtcct
    10321 cggagaagcg cttcaggtcc gcaccgccgg cgggcaggcc cttctcctcg atctttcggg
    10381 tgacctcgtc cacgatcttg gcgacatcga tatcctgccg gtcctccggc ctcggcggcg
    10441 gcaccagccc gatgttcagg ttctccggca gcgagaccgg cccgtcgaag gtgagcttga
    10501 tctcgctcgt tttcttcgcc ttgccctttc tgctcaacgc atccttggcg gtgcggcgaa
    10561 tatcctgcgt ttcctggaat tttttcaggt actcgatgtt cgcttcgatt ttccggttgg
    10621 tgttctcgtc gttgatgcgg ctgttgaccc cttcatacag ggagatcgcc gtcgcgaaat
    10681 cgctgttgcg ggcgtatatc tcggagttga tgagggtgcg cttgtagatc cggaaccgcg
    10741 acgaatcgga gatgatctcg tcggccttat agggcaactc gaagtcgtca tcgacctcct
    10801 gcccgcccgc ctcggaatcg gacttgcgcg aaggaccggt gcgctccgcc ggatcctgac
    10861 gcgcccgccg gtcgggttgg tcgggaaagc gcttctcgat ctcctggtcg gtcgcgcgcc
    10921 ttcggaacac gcgatccggc gactccacgt cgtccttgtc ggtgaggccc tccccctttc
    10981 cgaatagcgc ccatatcacg atcccgagca cggccacgag tgttactatg agtacgatat
    11041 acatacagct acatggtatc cgttaccgcg gcacacggca agccattatt cgtttatggg
    11101 gactgtatcg gtcacaagag gaagtatttt tcgggttttt cggggaaccc ccagcagatc
    11161 cgccagaaaa ccagcccatc gatctcctca cgcgaaagta tgtagtttga aaacggatag
    11221 ttatcggtca ttccccccga tctcgattga cggagaatat cgtcgaaata gtcacggacc
    11281 gcaatcgcca aaatcaacga atccgacatc cggtaaaagt tccgcaggtc cttgaagtaa
    11341 tcggcctcgt cctcgctgag tcgcacatgg acggtgcgcc acttctccct gcgatcgcgc
    11401 gattggtacc gcaccgagca cccgatccgc accgctcgcg acctatcgcg caaagcggcc
    11461 cggagcagca tcacgatgat ccggttgcgc gtccgcccgg tcagcgcgca tatctcgcaa
    11521 atcgagtcca gagtcgattc gtgaacatat agcgtcgtat cgatgggcat ggcatcctcc
    11581 aacggggccg tatactccta ttacgagcgc cggagaaaaa ccatgcagaa aaaaaataag
    11641 ccgggtcagg cgacacgctc gtcacgcacc gctaatcagc acgaccgtcg aaccgcgctg
    11701 aacgcgatag cggaggccgt cgagcgggcg ctcgcatcct gagggggcga tatcgtccgg
    11761 ctcgggaagc gccgtgtcca ccgcgacgcg ccacgcgccc cccgacggcg gcggcggaag
    11821 gaggaccgga tacgaaaaca gggacgcgtt aaaggccaag tagatatcaa catccggcgt
    11881 accgtattcg tcctcgacat aggccccgtt gaggaggcag gcgatgaagc ggctgccggg
    11941 gagccagtcg ggctcgcccg ggcgcgtccc gtgccaggag atgtcgggct ccggatgatc
    12001 gttatgcgca ctgccggtaa agaactcgtt tttacgaagg gcgtgatgtt ccttccggaa
    12061 gcggatcatc tcccggcaaa agcggacaat ctcacggtgc cgctccagac agccccagtc
    12121 gatccaggag atgtcgttgt cctggcagta ggcattgttg ttcccgcgct gcgtccggcg
    12181 gaactcgtcc cccgcgagaa gcatgggaac gccctgcgcc aggaagagcg tggcgatgaa
    12241 attcttgatc tgccgcaccc gccgccgctc gatgaagggc gtcacctcgg ccccctcgat
    12301 tccatagttc gcgctcagat tggtgttttc gccgtcccgg ttgccctccc cgttggcctc
    12361 attgtgcttg tcgcggaacg agaccaggtc gttcatcgtg aacccgtcgt ggcaggtgat
    12421 gaagttgatg ctgtgcagcg gccccttgtt ctcgccgcca tagaggtccg agctcccggt
    12481 gatccgcgtg gcgaagtacc ccacggtgtt ctcgtcgccg cgccagaatc gtcgcacatc
    12541 gtcccggaac tttccgttcc actcggccca acgcccggga aagttcccca cctggtaggc
    12601 ccccgccgcg tcccaggcct cggcgatgat cttcgtgctg cgaaggatcg ggtcctccat
    12661 gatcttttcg atgagtggcg ggttggccaa aatggctccg gtctggtcgc gacccaggat
    12721 ggaggccaga tcgaaccgaa agccgtccac gtgcatttcc accacccaga acttcaggca
    12781 gtcgatgatg aaatcgcgca cgagcggatg gttgcagttg atggtgttgc cgcagcccga
    12841 gaagttttta tacaaccggc ggttctcatc gagaatataa tagatcgaat tatcgatgcc
    12901 gcgaaacgaa agcgtgggcc ccatctggtc gccctcggcg gtatgattga acacgacgtc
    12961 caggatcacc tcgagccccg cctcgtgcag cgcgcacatc atgtccttga actcgtacac
    13021 ctgctcgccc atgcagcccg cgtgcgagta gcgcccccgc ggcgcgaaaa acgacatggt
    13081 gctgtagccc cagtaattgc gcaaccgctc gccggtggcg ggatccacgt tgatgttctc
    13141 cagctcgtcg aactcctgca ccggcatgag ctccaccgcg gtaaccccga gctccttgag
    13201 ataggggatc ttctcgatga tgccccggta ggtcccggcg aattcaacgc ctgatgacgg
    13261 atgcaccgaa aaccccctca catggagctc ataaataatc gtgtccgcga ggggggtctt
    13321 gagcggccga tcgaagaggt ccacctcggt gttcacgacg atgcagcgcg gagcgcacgc
    13381 ggcattgtcc gccttggaaa aggacagatc ggcctcgggc gagtccggat cgaacccgcg
    13441 cgagtgacgc aggtcccaga caggtccgcc tgcgatcgaa cgggcgtagg ggtccaggag
    13501 aagcttgtgc ggattgaagc gatgcccgcg aaacggcagg tagggcccgt ccatgcgata
    13561 gccgtagagc tggccctcgc tcacccccgc gatccacaga tgccagatgt cccccgtctt
    13621 gttgaggtgc ggatcgagca cgaactcctc gacgggggcg tcggggtccg ccgattcgaa
    13681 cagcaccaac gtaaccgatg aggcgtgacg gctaaaaacg gcgaactgcg ccccgttcat
    13741 gtcgagcttc acgcccaggg gactggggta tccgggaagg gtgatgcgtc cgggcttcat
    13801 ggtatcaggc gacctccgga aggactactc cctgacggtg gcgtgtaccc gcaaactgat
    13861 cttcggccgc tcggggtcgt tggtgaacac ctggatcgtc ttggacatcg taccggtata
    13921 tttgttcgtg tacagcgtca cttcaatctt cccctcgccg ccggcgggga tctccttgct
    13981 cgagagcagg gccgctgcgc accctcaggt gggctgaacc cgttctatca ccagcgtccg
    14041 cttgcccgca ttgcggaatg cgaacacggc ctgaagcgac ttttcctgct caacggcgcc
    14101 gaagtcgaac gtctcgaatt cgaacacgac accgggatca tcacacgaca cgagcgtcac
    14161 aacggcgctc agcatgatga taatgagcgg cagtaatcga accttcatac atgcacatcg
    14221 ctgcagcagg tgagtaaatg caagtatttt ttcgcccaac cgggccaact gcctcaagcg
    14281 cccggctcgg tccgcccgtc cgcgttgatc gtggcctcgg cgacggcgcg ctcgagattc
    14341 cgccgcgcgt cctcgagcgg atcagccgag ccctcctcga tcgacgcgat tcccgtgaag
    14401 gggcgaagcg gataggactt cttgtcgatc gtgatccgaa cattcgaaag cgcccgcagt
    14461 acgccctcct cggccgctgc cgcctcgcgc gcgccggcgc ccttcatcaa caccacgaaa
    14521 tcgtcccccg cgctcaggac gtcatccgcg cgcaggtgct cgtagatccg ctcggcgacc
    14581 gcctgaagca gcagccgccg ctcctcgctc ccctcctccg gggtcccgtt gatcgccttg
    14641 acggccatca ccgcaagcgg gacgcgggcg ccgcgtggct ggttcaacag ctcatcgtat
    14701 ttcagcttga ggaaggcgcg gctgtacacg cccgtgtcgc ggtccaccat cacgtcgttg
    14761 agcagcatgt attcgttgat gaacctcgcc atcgccacga tctcatcgcg gatgtcgtgg
    14821 atctcggccc ccgagaaggg aagctggcgg agtatcacca ccgcgcccat cagcgcgtcg
    14881 cggtacagga ccggcgcgat cacccgccgg ccgccgtcga gcatgagcag cgattcattg
    14941 cgcagcccct cgccgatctc gttgtcgacg tcgatggcct cgtagcttcc ggaatcgacc
    15001 ttgagaaagg cctgcccgtt gagttcatag cgcttctcga gacggctcgc gcggtcgcgc
    15061 gcgatgtaga gcgagaccgc gtccggcgtg gtgcgcgcgg cgatcgcgga aaatccctcg
    15121 tagacgtgtg cgtccagcga cgccgcggcc gcattgcgca tatcgcgata gatgggactg
    15181 ttctcgcggt ttttcgcgag tcgctttcgg tccaccgtca cgtcccggta cggctccgat
    15241 tcctccgtcg gctcctccgt gacaggtttc cgtttggcta atagaatgta caccacggtg
    15301 agcaccagca ccacgcccat gcacagaagc gcgagctcga gccccagccg caccttcgcc
    15361 gccgcggtca tgcggtaggg gaagatgatg aggatgcgcc cctccgtgac gcttttcacg
    15421 aacagatagc acttcgcccg gttatagtag cgcaccacga actgccggcc cggatcgggc
    15481 gcgagggcgc cctcgcggaa ttcgttgatg atgctgtcgt acaactcgtc cgcggcaatc
    15541 gcctgataga gcttcttgtc ggcaatgacc cggttctcgc ggtcgaacac cgcgatgagc
    15601 tcggggcgat acttcgcgct gatggcgacc accgccgtgg gcatctccgc ctcggggcgg
    15661 cgcggatcgg cccccttgaa cacgcggttc accgcgagcg cgatccggtt gctgatatcg
    15721 accagcgatt cggtgtagtc ccgctccagc atgtcggagc gcagcgaggt gaagccgagc
    15781 gacatcacga tgatgccgag aacgccgatg aagatgagcc agcgcgcgag tgtcattgat
    15841 agatccctga tttgtagtat gggccttctc tatcaataat cggtcacaga atcgcgaatt
    15901 tccccataat ttacggattt tcctgcgttt tttatagtca cccgcgcgtc cgaaccgggg
    15961 cgaaacggaa tcgccgaacg gcgctcaacc ggacgctgtg cgaacgcggg tatttccccg
    16021 ctcgtgtcag cctatcaaaa cgatcgattc gctacgcacg cgcctcagcg ccgagctgta
    16081 accgaaggcc tgttcccttc cctttgcacc tgtatcaaca ctgacccgga tgatgacgat
    16141 tccggcggtg acgggatcag cgaaaaaaaa tattactaaa catatttttg gctaaacgta
    16201 gatgtagttt ttccgataga tattctgtga gtgacataat cgcagaacga tcgcagcagg
    16261 gaggtaccgc atgaagatat acggattttc cacaagcagc aaactgaacg accgccggcc
    16321 gcacgaacat catgtctggg gttcaagcgc cctcaagcgg gagcgcagcg atacggacag
    16381 cgttgtgata tcgcccgagg cgcgagagcg ctttgcggcc gcgcgcgcca tcgacatgga
    16441 acatgcgcga tcgcggcggc ttgcccgcga gctcgccccc gcgattcgcc gggcgatcga
    16501 tgccggtggc gaaaccgacg acgcccgcgc ggaccgcatc gaggccctcc ggcgcgcatt
    16561 tgcgacgggg gacgcgcgaa tgagcgacac ggcgctgcga gacacggccg acgcgctcct
    16621 ggcgctggcg atgtgagcga ccgggcctct aatcaaacgg ttgcaccaga agccctgcgc
    16681 tcatcagccc gatcggtttg taggcatcct cgcgggcccc ggtctggctg aacgtgaagg
    16741 agaggtagtt gacgccgccc tgcaggctca cgtacggctg caaccagtcg aaaaccggga
    16801 agtacaatcc cggtcccacg gtaacggtga cctggctcag atatgaaccg gggctaaagt
    16861 tcacatcata ccgtccgtag aggagattgc tctcgaaaag gatcatgttc caggcggaga
    16921 agtacaacag ggttgcgccg tagccaatac ccggattgag gtccttctgg aatgtgcccc
    16981 cggggaccag gtagcccccg cccaggccgg cgagaaagcc gcgggtattc gataccgatt
    17041 cgtcggtcgt cgggaaaacg gtggcgctgg gtccgaatga ccacaggagc accgcgacaa
    17101 tcgcccggca cgcatatcgc gacgttcggg aaaaacgata cccacgtttc atttttctct
    17161 tcgccgaaat ggcgacacta accgcattat tcacccgaac aattcaggtg agttggccag
    17221 tgcggccgca tcgggtttcc tatcgtcaaa tatatccgca caaatctatt acaatagcca
    17281 taccgcaaaa ctggcaattt ctgcgtttat tgcgcatcaa tccccttgtt gcacataggg
    17341 aaccatgctt attggttaat aaaccctctt tcccgcagtt gctctgattt cttcatcgcc
    17401 atcgctcgtc gcgatggcat taacacgaaa cgcgcgacgt atatagtata caaaatcttg
    17461 cattgaatag tcaacccagt tgggcgtttt ttcccgatgc catttccaaa aattaataca
    17521 tgcctcatca aaccggcttt ctcactcgat taaaaaaaat ccaacaagtg aaatggtcct
    17581 cgcaagtcat tttttatacc aaaacttcaa gtttttaaaa aacaatcaaa atggatcaaa
    17641 acatatgata ttttgccgta ttttggaata atctccccag aattcattaa aaaaaccgcg
    17701 atcgaattaa attttacaga ctttggggcc gatcatacga gcaaggtgaa ttgtcacctt
    17761 atggttcttt gaaataaccc gggttgtgat gcgataatca cggggcgacc tttgttcgcg
    17821 ttccaagctt atattggccc aaatacaagc ctggaacagg cgcactcgtc ccggccgtta
    17881 actgcggccg caatcggtat caagctaccg gcctacatcc gccctcacgg gtcggtatgg
    17941 caaggaagcc agaacacata gaacaaagga gtgtaaaatg attatcaatc acaacatgag
    18001 tgcaatcaac gcgaaccggt cgctcaagtt cacgtcgtgg gaagtgggca agagcatgca
    18061 gaagctggcg tcgggcctcc gtatcaacaa ggcgggcgac gacgcttccg g
//
LOCUS       DAORHF010000046        17600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2315_length_17600_cov_6.230493, whole genome shotgun sequence.
ACCESSION   DAORHF010000046 DAORHF010000000
VERSION     DAORHF010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17600
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2315_length_17600_cov_6.230493"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(152..475)
                     /locus_tag="PKJ16_05975"
     CDS             complement(152..475)
                     /locus_tag="PKJ16_05975"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HNV46571.1"
                     /translation="MEVRQYHVYLVNLDPTVGHEIKKTRPCVVISPDEMNCAIRTVII
                     APLTSRSHKYPTRVQLSFQGAKGWIVLDQVRTIDQQRMIRELGRIGEPVIREVKKIIR
                     EMLVD"
     gene            complement(477..722)
                     /locus_tag="PKJ16_05980"
     CDS             complement(477..722)
                     /locus_tag="PKJ16_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV46572.1"
                     /translation="MIVKIVPIGNSRGIRIPAEILKQFRMDSQVELIVNEGSGSITLR
                     PVRTPRTGWDEAFRRMRERSEDDLIIDDDIDLCEWEW"
     gene            947..2695
                     /locus_tag="PKJ16_05985"
     CDS             947..2695
                     /locus_tag="PKJ16_05985"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HNV46573.1"
                     /translation="MDATVQKRIQEWTTPPFDAATIAEVKALVDAKDEKELTERFYAM
                     LEFGTGGLRGVLGAGTNRMNAHTVGMAAQGLADYIAAQGQQKKGVVIARDSRRMSDEF
                     SRVAAEIMAGNGIVAYVFDDIAPTPLCSFAIRELKAVSGIVITASHNPPEYNGFKAYW
                     DDGGQVVPPHDTAIIACVRKIESITQIKKLSWDEGIAKGLIKIIGQDIIGAYIASLEK
                     AALRPRAASELKIAYSPLHGTGYRIMPQVLAHFGFANVTLESAQAKPDGNFPTVKYPN
                     PEEREAMQLLIELAKKTNADILVATDPDADRMGVGFRDERGEYLLINGNQIGTMLEYY
                     LLKRLSAEGRLPKNGAVVKTIVTTDLQEEIAAHFGCVADNVLTGFKWIALKMKEYEDA
                     GSRTFIFGGEESYGYLPVPFVRDKDAIAACYFFAEMADWLKGQGITLNDFLNEIYATF
                     GLYLEGLHSLTLKGIEGIERIKGMMASLRALPPAEFAGVKVTRVDDIQNLVRTDCATG
                     GTTAIADLPKSDVLQFFLADGTKITMRPSGTEPKIKFYFSVKEKTDAKGLAAAKIKLA
                     ERIDVLTKDLLKKVEA"
     gene            complement(2729..4354)
                     /locus_tag="PKJ16_05990"
     CDS             complement(2729..4354)
                     /locus_tag="PKJ16_05990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV46574.1"
                     /translation="MKILLAYLCNRVDADDYWLSLAPVGLVSLAACLEGAGHDVTLAN
                     FSKRGYRAGAREAAAIAPEAVGVSIFTHNRAEALAFVREVKRLLPAATVIVGGPHASF
                     LAGEILRRVPAVDYVVTGEAEHAVIDLINTLKKGTVPPARIIPGGRIAHVDTLPAPGL
                     FGGRLLHVDPNEQFKYLVTTRGCAHACVFCSSPGFWLRTTSFRSARDIVDEMRRLHDA
                     YGIIYFSIRDDNFTQKKGRVMEFARLLGSSGMYVMWNCQARVDAVDREMLVAMKRAGL
                     EHIQYGVESGSPAVLARYDKKTSAEAIVRAAEATRAAGIYLSIYLMAGMAGETHADIR
                     KTVALIRKILPGDGIVSPVALYPGTALYEDEKRAGRIHDADWFSRKDAGIFLRNEPVV
                     AEWMNELLHAIAQVRGRAWYAEADFRRHRELCGTDCWVTDILEGDYWLDEERTAKARA
                     AYRRVTESRPRNPWGHFRLGKLSFRDEDFAGAAECYREVTRIVPAFYGGWLKLAEAQL
                     ALGNRRDARESAREAQRRNAYDERIRNVLSLLK"
     gene            complement(4351..5061)
                     /locus_tag="PKJ16_05995"
     CDS             complement(4351..5061)
                     /locus_tag="PKJ16_05995"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV46575.1"
                     /translation="MIGARISGATGRIIRAIRTLPIILVGVAIVAIPVAAEKKPEAVP
                     IAGFDINLREKPSLDSRVIGRLSFGHEVRVLDSSAQEVEIDGKRGRWTLVERLYCGNE
                     GDACRGWLFDHYLAYRHRLKRVASWPHREFDNFLGGDAHRYYAFTPDGGFVESLHLGS
                     TRQETDTSAVKQCNSFKGSLEKEKSRDVSEPGMVYYVCKRKGHLYRYLNLVCPEGGAC
                     FMVMPDGRLMSVGAYLKQ"
     gene            complement(5063..5665)
                     /locus_tag="PKJ16_06000"
     CDS             complement(5063..5665)
                     /locus_tag="PKJ16_06000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014587028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV46576.1"
                     /translation="MNISIILGHPSSGSFNHAIADAARDFFAGRGDRVFYHDLYAERF
                     DPVLPAGEIPRDGRVDAAIGAYCDELALSDGIVIVHPNWWGQPPAILKGWVDRVVRPG
                     VAYAFEEADGGEGVPRGLLRARFAAVFNTSNTPEARERDVFGDPLELLWKNCIFDLCG
                     VTRFYRTMYRVVCTSTPVERAAWLDEVRSILGGMLGGEAR"
     gene            complement(5706..6350)
                     /locus_tag="PKJ16_06005"
     CDS             complement(5706..6350)
                     /locus_tag="PKJ16_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-D-aspartate receptor NMDAR2C subunit"
                     /protein_id="HNV46577.1"
                     /translation="MGDKVDDAHLGRRWLSLFPADADRAALGRAFESLIARYREPHRR
                     YHAVSHLAHCLEQFDQIGSLAESPRALELALWHHDAIYRTTSFRNEERSARLAAATLS
                     ALDPAGCETGVVRELIVLTRHDRVPPSVDGRFMVDIDLTILGAPDDAYRRYEEGVRRE
                     YGWVPGFLYRKMRSKLLGALLVRDRIYHSDYFHNRYEQAARANLAEEMGRLLRQ"
     gene            complement(6355..6978)
                     /locus_tag="PKJ16_06010"
     CDS             complement(6355..6978)
                     /locus_tag="PKJ16_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46578.1"
                     /translation="MNQPELTQKALGILRNPETFQWYVIPLLACVAYIYFNEIEKRNW
                     RAVAAGLSLYMVHWFFEIGNALIQHFTGHALWTVPTGTSFLLLVGVCVEISFMFSVAG
                     VIFTKILPADPKMKILGVNNRIFLAIVNAAFFSIFEIFLARTPAFHWVYPWWGSFPVF
                     VTVYIPFFLAANLAYDWAPRRQRAFIGGLFVVNAIMLAVFAGILKWI"
     gene            complement(7036..7827)
                     /locus_tag="PKJ16_06015"
     CDS             complement(7036..7827)
                     /locus_tag="PKJ16_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HNV46579.1"
                     /translation="MYRITIRFYEELNDFLPRGKRKRDVDVAFPGRRSVKDLIESLGV
                     PHGEVDLVLVNGASVDFSRIVADGDRISVYPVFESLDILPVTRLRTAPLRDTRFVLDV
                     HLRKLARRLRLLGFDVDYRENRGDAELAEISTRERRILLTRDRRLLMRRIVERGVFVR
                     ATRIDAQVADVLDRLDLRGSISPFTRCIECNGRMRSLPIDSPECSPALDAVPPGVRAW
                     CREFHRCDGCGRVYWKGSHYDRLRRVVEALDVPPAGERSADRFKK"
     gene            8058..8906
                     /locus_tag="PKJ16_06020"
     CDS             8058..8906
                     /locus_tag="PKJ16_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV46580.1"
                     /translation="MPSAERGSRLHDGKRAETRPTALITGGSSGIGKELARLFHRAGY
                     RVLIAALDRTELAQTKRELLAAGNDTRIDTLTLDLSRQGAAARIVRWTRSLGAEVDVL
                     VNNAGFGLWGQAVELPLPEVRSMLAVNITAATELCHAIGGLMKARGRGSILNVASTAS
                     FQPVPYMAAYAASKCYLASFTAALAAELRPFGVAVSILHPGTTRTNFLSRAGINPGSR
                     ASLGGLAHAIAMDPADVAAAGFAAVLHGRRRAIPGALNRLQFLATRLLPGFAIMAMAK
                     KIFKKP"
     gene            8927..9202
                     /locus_tag="PKJ16_06025"
     CDS             8927..9202
                     /locus_tag="PKJ16_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46581.1"
                     /translation="MTFTDEPVYYRLVPHAGAGYAQEVTHDMQITYKCRNGHKFKKEE
                     APNVTCSSCGEPAEAVKWNTVESYAIPEKGLGVMDEINSVIKEYRKK"
     gene            complement(9234..9491)
                     /locus_tag="PKJ16_06030"
     CDS             complement(9234..9491)
                     /locus_tag="PKJ16_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46582.1"
                     /translation="MKRFVMILAAAGLLVMSGEGVLRAENPCQSECEAALGRCLKDCE
                     RAVDPDELKKKSAEVTPGAAKKKCAKQCYATRRACWRSCAR"
     gene            complement(9488..9982)
                     /locus_tag="PKJ16_06035"
     CDS             complement(9488..9982)
                     /locus_tag="PKJ16_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV46583.1"
                     /translation="MKFVCTLLVVEDIARARAFYETVLGQTVKFDFGDNVTFAGDFAI
                     HRKSHYRGLIGDLEVARGRNDVELYFEYDDVEAMEKRLDGQAVRFLHKTREQPWRQRV
                     LRVYDPDGYIVEIGESLGHLCYRLSLDGIAEPEIAETVGIPEGMVREGIARYGASKQR
                     GVEQ"
     gene            10632..13418
                     /locus_tag="PKJ16_06040"
     CDS             10632..13418
                     /locus_tag="PKJ16_06040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46584.1"
                     /translation="MNNDTEKLQQQEIDRLNRRIAELEIIIKTTDELEYSYIQTHDRY
                     RTILEEMNEEYFETDLEGNFTFLNDSTVRTLGYAKDELVGSNYGILVHPMERDRIFSI
                     FGTIYATGAGLKDFTWACIDKLGREHIMRASVVPLKDHHGATIGFKGIAQDITEKIEA
                     ERKLKEEEELYRTIFNTTSNPTIIIDEDTTILLVNREFERLTGLPAADIEGKRRWTEF
                     VVTDDRERMLAHHRDRRTDGGNAPSNYEFRFCGATGALRDIYLSISLIPGTMKAVASL
                     LDITERKQADIELRRSEKRFRGIFENAVEGIFQVTPDGRLVTANPALATILGFDDPGS
                     AIAALADFGGSVFADPGGFDDFGQRLGREHAIKAYETRFRRRDGAVIDVIINAHTVRD
                     GQRAVAMYEGMLQDVTSVKLAEKLRLSTEAAEAANRSKSEFLANMSHEIRTPMNAILG
                     FAELLEGKIDDPVLRQYIASIRSSGTMLLGLINDILDLSKIEAGKFEIRARPVDPHAV
                     FSEIRQLFAARIAERGVAFILDIDPGLPPSLVLDEVRMRQILFNLVGNALKFTDRGRI
                     TLTACRLDNRDDADAIDLLVSVADTGIGIPPEQHDLIFESFRQAEGGRASAIAGSGLG
                     LAITKRLVEMMNGAITVESEPGDGSVFTVVLRGVAVADEADGADTAAPDAMPQYAFDR
                     ARALIVDDIETNRMLLRGYLALYDIDCAESADGREAMEQSRRNPPDVVLMDMKMPVMS
                     GYDAIRAMKADPALRDIPVIAVSASTTLRDVEIIEQTGCDAFLRKPVSRADVIAAIAR
                     FLPHRIVEPTDRRPAEESAVPLMRAMDDEARMRLPELIERLEGDCMARWLRIQKTFII
                     NEVKEFARTMSALGSAYAVPPLDDWGAALMREAASFDMNALPSTLKRYEELLVAIKGA
                     TEGPAIPTNDRPQE"
     gene            13424..14431
                     /locus_tag="PKJ16_06045"
     CDS             13424..14431
                     /locus_tag="PKJ16_06045"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV46585.1"
                     /translation="MEPMTASSLNAKPLILIVDDAPKNLQVLGAMLDERSYEVAVASN
                     GAQAISLLSEIRPDLILLDVMMPVMDGFQTCRMIKESPETRDIPVIFLTARDETESII
                     TGFRLGAVDYITKPFSSYELLARVDAHVELKRCRDNIVECNNRLNLEIEYRKQIAHEN
                     EALLNDLLNNNRRLENLAVTDSLTGLYNHNVIIDRLEGEVNMAHRYQTPLSVIMFDVD
                     HFKRINDTYGHLIGDEVLVRISSVMRNRLRRVDVSGRYGGEEFLVILPHTEMKNGYFI
                     ADKLRKAVKDQQWSRSGLEVTISGGIAEFQDENPRDLIEKADRLLYKAKEHGRNRIEF
                     N"
     gene            14403..15614
                     /locus_tag="PKJ16_06050"
     CDS             14403..15614
                     /locus_tag="PKJ16_06050"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HNV46586.1"
                     /translation="MAETASNSTDARTPLVLIVDDNPGNLQVLGSVLSGAEIDVAIAT
                     GGREALASVEEALPDLILLDLMMPGLDGLQVCRALKESERTCDVPVMFLTARHETDDV
                     LQGFQAGAVDYVCKPFNAPELLARVRTHLELRLVKQRQREIIRELERAQADLKKKYDI
                     ICSDLALAKKIQGRMFPDDIDSIIGYAFDIRYLPVEDVGGDYFDICRLNNGVVRIMLA
                     DATGHGIQAALITMLIKSEYEKLKEIIRKPSEVLELLNNVFLRTYRTMGILFTGTILD
                     IHPGRDRLAYSSAGQTPALLLHENRIEPLGETGRMIGVLEGERYRTVEHDLSAATRML
                     LYSDGLYEQFNAKGEMFGDERMIAYIDSIRTQPTGAIIDRLLAEVMRFRGTSERITRN
                     DDITVIGVSPR"
     gene            complement(15633..15872)
                     /locus_tag="PKJ16_06055"
     CDS             complement(15633..15872)
                     /locus_tag="PKJ16_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46587.1"
                     /translation="MKRGTCPKCGSATVYLLEDGIGSAECQYIKKGGFFTSSTTFDCY
                     LCVSCGFFENYVNNRQHLETIAQRGEWKRVPPHGA"
     gene            16048..16284
                     /locus_tag="PKJ16_06060"
     CDS             16048..16284
                     /locus_tag="PKJ16_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HNV46588.1"
                     /translation="MDCSACGGLLIEGKSCYTASKDHYSLVLEDIPAFQCTRCGRVLF
                     AAETVDKIETMVKRIERESREIVSGKPTTNLYDF"
     gene            complement(16304..>17600)
                     /gene="glnS"
                     /locus_tag="PKJ16_06065"
     CDS             complement(16304..>17600)
                     /gene="glnS"
                     /locus_tag="PKJ16_06065"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HNV46589.1"
                     /translation="YRGTLKEPGRPSPYRDRSVEENLDLFARMKNGEFPDGSKTLRAK
                     IDMAASFIVMRDPVMYRILRATHHRTGDMWCIYPMYDFTHCLSDALEGITHSICTLEF
                     ENNRALYDWFLAALDTPNRPQQIEFARLNLSYTVMSKRKLLQLVKEGRVAGWDDPRMP
                     TIAGMRRRGYTPEAIRDFAERIGVAKADSTVEFSYLEHCVRDDLNRRAHRVMAVLDPL
                     KVVIENYPEDALEELDAENNPENASAGTRKIPFSRTLYVERDDFREDPPKKYHRLSPG
                     AEVRLKSAYIIKCEKVIKDAAGNVVELRCTYDPTTKSGADNSGKKVKGTLHWVSAAHG
                     LTAEARLYNPLFTKENPDDVAEGENFLDAINPQSLETLPGIFIEPSVKGAAPGAIFQF
                     LRHGYFCVDPDSTEARLVFNRTVQLRDSWAKIEKAGGAG"
BASE COUNT         3436 a         5797 c         5409 g         2958 t
ORIGIN      
        1 ttctacggcc ccggcgcggg ggtgcggctg tatctcaaat cggtggcgat ccccgccttc
       61 ggcttcgacg tggcctacaa cctccggcag gggaacgtcg agttcacctt cgccatcggc
      121 atgcagatgt agcggcgtgc atgcgaagcg atcaatcaac aagcatttcc ctgatgattt
      181 ttttcacctc tcggataacc ggttcgccga tgcgtccaag ctccctgatc atccgttgct
      241 gatcgattgt gcgcacctgg tcgagcacga tccagccctt tgcaccctgg aatgacagct
      301 gaacgcgagt tgggtactta tgggaacgcg acgtcaatgg ggcgatgatg accgtcctga
      361 ttgcgcagtt catttcatcg ggcgagatca ccacgcaggg gcgtgttttt ttaatctcat
      421 gaccaacggt cggatcgaga ttgacaagat agacatgata ctgtcgtacc tccatgctac
      481 cactcccatt cgcaaaggtc gatgtcatcg tcgatgatca ggtcgtcctc gctccgttcg
      541 cgcatccgac gaaacgcctc atcccacccc gtgcgcggcg tgcgcaccgg acgcagggtg
      601 atcgatcccg agccctcatt gacgatcagc tcgacctgcg aatccattct gaactgcttc
      661 aggatctcgg cagggatgcg aataccgcgt gaattgccga tcggaacaat cttgactatc
      721 atggcacccc ctgaatgaat gtaattacaa tgtaataata ttcacccatt cgtgtcaagc
      781 aattccccgg agcatttcac tggagcaccg cacccccttc tccccgacaa tgccatcgta
      841 atctttatac ggtggtaacc atgaaaagga taaaaatact tgatcaaata cgaaaaaaac
      901 ctacgctaag cagaaaccga taagcaaata ctcccccgga ggaaccatgg acgcgaccgt
      961 gcagaaacga attcaggaat ggaccacgcc ccccttcgac gcggcgacga tcgccgaggt
     1021 gaaggcgctc gttgacgcca aggacgaaaa ggagctcacc gagcgttttt acgccatgct
     1081 cgagttcggc acgggcggcc tgcgcggggt gctgggcgcc ggcaccaacc gcatgaacgc
     1141 gcacaccgtg ggcatggcgg cgcagggcct ggccgactac atcgccgcgc aggggcagca
     1201 aaaaaagggc gtggtgatcg cgcgcgactc gcgccgcatg tccgacgagt tttcacgggt
     1261 cgcggcggag atcatggccg gaaacggcat cgtcgcttac gtcttcgacg acatcgcgcc
     1321 caccccgctg tgctcgttcg ccatccgcga actcaaggcg gtctcgggga tcgtcatcac
     1381 cgcgagccac aaccccccgg agtacaacgg cttcaaggcc tactgggacg acggcggcca
     1441 ggtggtcccg ccgcacgaca cggccatcat cgcgtgcgta cgcaagatcg agtcgatcac
     1501 gcaaatcaag aagctatcgt gggacgaggg gatcgcgaag ggcctcatta aaatcatcgg
     1561 ccaggacatc atcggcgcct acatcgcgag cctcgagaag gccgcgctgc gcccgcgggc
     1621 ggcctcggag ctgaagatcg cctacagtcc gctccacggc accggataca gaatcatgcc
     1681 gcaggtgctc gcgcacttcg gcttcgccaa cgtgacgctg gagagcgcgc aggcgaagcc
     1741 cgacggaaac ttcccgacgg tgaaataccc caaccccgag gagcgcgagg cgatgcagct
     1801 cctcatcgag ctcgcgaaga agacgaacgc cgacatcctc gtcgcgaccg atcccgacgc
     1861 cgaccgcatg ggcgtgggct tccgcgacga gcgcggcgag tacctcctca tcaacggcaa
     1921 ccagatcggc accatgctgg agtactacct cctcaagcgc ctgagcgccg agggtcgcct
     1981 gccgaaaaac ggcgcggtcg tcaagaccat cgtgaccacg gacctccagg aggagatcgc
     2041 cgcgcacttc ggctgcgttg cggacaacgt gctcaccggc ttcaagtgga tcgccctcaa
     2101 gatgaaggag tacgaggacg cgggatcgcg caccttcatc ttcggcgggg aggagagcta
     2161 cggctatctg ccggtcccgt tcgtgcgcga caaggacgcc atcgccgcat gctacttctt
     2221 cgccgagatg gccgactggc tcaagggaca gggcatcacc ctgaacgatt tcctgaacga
     2281 gatctacgcc accttcggcc tctacctcga ggggctgcac tcgctcacgc tcaagggcat
     2341 cgaggggatc gaacgcatca aggggatgat ggcgtcgctg cgcgcgctgc cgcccgcgga
     2401 gttcgccggc gtgaaagtga cgcgcgtgga cgatatccag aacctggtgc gcaccgactg
     2461 cgccacgggc ggaacaaccg ccatcgcgga cctgccgaaa tcggacgtgt tgcagttttt
     2521 tcttgccgac ggaaccaaga tcaccatgcg cccctccggg accgaaccca agatcaagtt
     2581 ctacttcagc gtgaaggaga agaccgacgc gaaggggctg gcagcggcga agataaagct
     2641 cgccgagcgc atcgacgtgc tcacgaagga tctgctgaaa aaggtggaag catagcgcac
     2701 gcaatgacag cgatgcgcct gggtgcggtt acttcagcag cgagagcacg ttcctgatcc
     2761 gctcgtcata ggcgttgcgc cgctgcgcct cccgggcgct ctcgcgggcg tcgcggcggt
     2821 ttcccagcgc aagctgggcc tcggccagct tgagccagcc gccgtagaag gcgggcacga
     2881 tacgggtcac ctcgcgatag cattccgccg ccccggcgaa gtcctcgtcc cgaaacgaaa
     2941 gcttgcccaa tcggaaatgc ccccacggat tgcgcgggcg ggactccgtg acgcgccggt
     3001 aggccgcgcg cgcctttgcc gtccgctcct cgtccagcca gtagtccccc tcgaggatgt
     3061 ccgtcaccca gcagtcggtc ccgcagagct cgcgatggcg gcggaaatcg gcctccgcgt
     3121 accaggcgcg gccgcgcacc tgcgcgatcg cgtgcaggag ctcgttcatc cactccgcga
     3181 cgaccggctc attgcgcagg aagatcccgg cgtccttccg cgagaaccag tcggcatcat
     3241 ggatgcggcc ggcccgtttc tcgtcctcgt agagcgcggt gccgggatag agcgcgaccg
     3301 gcgagacgat gccgtcgccc ggcaggatct tcctgatgag cgcgacggtc ttgcggatgt
     3361 cggcgtgcgt ctcgcccgcc atgcccgcca tgaggtaaat ggaaagatag atccccgccg
     3421 cgcgcgtcgc ctccgcggcc cggacgatcg cctccgccga ggtcttcttg tcgtagcgcg
     3481 cgaggaccgc cggcgagccc gactccacgc cgtactggat gtgctcgagc cccgcgcgct
     3541 tcatggcgac gagcatctcc cggtcgaccg cgtccacgcg cgcctggcag ttccacataa
     3601 cgtacatgcc gctcgacccg agcaggcgcg cgaactccat cacccggccc ttcttctgcg
     3661 tgaagttgtc gtcgcgtatc gagaagtaga tgatgccgta ggcgtcgtgc aggcggcgca
     3721 tctcgtccac gatgtcgcgc gcgctccgga aggacgttgt gcgcagccag aagccgggcg
     3781 acgagcagaa gacgcaggcg tgcgcgcacc cccgcgtggt gaccaggtac ttgaactgct
     3841 cgttgggatc gacgtgcagc agccggccgc cgaagagacc cggggccggc agcgtatcga
     3901 catgcgcgat acgtccgccc ggaattatgc gcgccggcgg caccgttcct tttttaagcg
     3961 tgttgatgag atcgatgacg gcatgctccg cctcgccggt taccacgtaa tcgaccgcgg
     4021 gaacgcggcg gaggatctcc ccggcgagga acgatgcgtg cggaccgccg acgatcaccg
     4081 tcgcggccgg caggagcctc tttacctcgc ggacgaaggc gagcgcctcg gcccgattat
     4141 gcgtgaatat cgatacgccg accgcctccg gcgcgatagc ggccgcctcg cgcgcccccg
     4201 cgcgatatcc acgcttcgag aagttcgcga gcgtcacgtc gtgcccggcg ccctcgaggc
     4261 aggcggcaag cgagacgagg cccaccggcg caagcgagag ccagtagtcg tccgcgtcga
     4321 cgcgattgca gagatacgcc aagagtatct tcattgtttg agatacgcac ccaccgacat
     4381 gagccttcca tccggcataa ccatgaagca ggccccgccc tccgggcaga ccaaattcaa
     4441 gtagcggtac aggtggccct tccttttgca tacgtaatag accatgcccg gctcgcttac
     4501 gtccctcgat ttttcctttt ccagtgagcc cttgaacgaa ttgcactgct tcacggcgga
     4561 cgtatcggtc tcctgccgcg tcgatcccag gtggagcgat tcgacgaaac cgccgtcggg
     4621 cgtgaacgca tagtaacggt gcgcgtcccc gccgaggaag ttgtcgaact cccgatgggg
     4681 ccaggaggcg accctcttca ggcgatgccg ataggcgagg tagtgatcga acaaccaccc
     4741 ccggcacgca tccccctcgt tgccgcaata caatcgctcg accagcgtcc atcggccccg
     4801 tttcccgtcg atctcgacct cctgcgcact cgaatcgaga acgcgcacct catggccgaa
     4861 gctcaggcgg ccgatgaccc tcgaatcgag cgacggcttt tcccgcaggt tgatgtcgaa
     4921 tccggcgatg gggacggcct cgggtttctt ttctgcggcg acagggatgg cgacaatcgc
     4981 gacaccgacg aggatgatgg gcagcgtccg tatcgcccgt atgattcgtc ccgtcgcccc
     5041 gctgatgcgc gcaccaatca tatcaacgcg cctcgccgcc gagcattcca cccaggatgc
     5101 tccgaacctc atcgagccac gccgcgcgct cgacgggcgt actcgtacag acaacacggt
     5161 acatcgtcct atagaagcgc gtcacgccgc acaaatcgaa gatgcagttt ttccacagaa
     5221 gctcgagcgg atcgccgaag acgtctcgtt cgcgcgcctc gggcgtattg gacgtattga
     5281 agacggccgc gaagcgcgcc cgcagcagcc cccgcggcac gccctcgcct ccatcggcct
     5341 cctcgaatgc atacgcgacg cccggacgga ccacgcgatc gacccacccc ttgaggatgg
     5401 cgggcggctg cccccaccag ttgggatgca cgatgacgat gccgtcggag agcgcgagct
     5461 cgtcgcagta tgcgccgatc gccgcatcga cgcggccgtc ccgtggaatc tctcccgccg
     5521 gcagaacggg atcgaagcgt tccgcataca gatcgtgata aaagacgcgg tcgccgcgcc
     5581 ccgcgaagaa atcgcgcgcc gcgtccgcga tggcgtgatt gaaactgccc gatgacggat
     5641 gtccgagtat gatcgatata ttcatgttct ctcccacgcg tttcgccgcc gatgaggccg
     5701 acgcctcatt gacgcagcaa tcttcccatc tcctccgcaa ggttcgcccg cgcggcctgt
     5761 tcgtatcggt tgtgaaaata atcggaatga tagatccgat cgcgcaccag cagggccccc
     5821 agaagcttcg accgcatctt ccggtataga aaacccggaa cccacccgta ctcgcgccgc
     5881 acgccctcct cgtaccgccg atacgcgtcg tccggcgcac cgaggatcgt aaggtcgatg
     5941 tcgaccatga agcgcccgtc gaccgatggc ggcacgcgat cgtgcctcgt gagcacgatc
     6001 agctcgcgga ccacgcccgt ctcgcacccg gcgggatcga gcgccgagag cgttgccgcg
     6061 gccagtcggg cgctgcgctc ctcattgcgg aatgacgttg tgcgatatat cgcatcatgg
     6121 tgccacagcg cgagctcgag ggcgcgcggc gattcggcca ggctcccgat ctggtcgaac
     6181 tgctccaggc agtgggccag atgggacacg gcatgatagc gccggtgcgg ctcgcggtag
     6241 cgggcgatca gcgattcgaa ggcccgcccc agcgccgccc ggtcggcatc cgccgggaag
     6301 agcgagagcc agcgccgccc gaggtgcgca tcatccacct tgtcgcccat ggcgtcagat
     6361 ccatttcagg atgccggcaa acaccgccag catgatcgcg ttaacgacga acaacccgcc
     6421 gatgaacgcc ctctgccgcc gcggcgccca gtcgtaggcg aggttcgccg cgaggaagaa
     6481 cgggatgtac accgtcacga acaccgggaa cgacccccac cacggataga cccagtggaa
     6541 ggccggcgtg cgcgcgagga atatctcgaa gatcgagaag aaagcggcat tgacgatggc
     6601 caggaaaatc cggttgttga cgcccaggat cttcatcttc ggatcggcgg ggaggatctt
     6661 cgtgaagatg acgcccgcga cggagaacat gaaactgatc tccacgcaca cgcccaccag
     6721 cagcagaaac gacgtcccgg tcgggaccgt ccagagggcg tgcccggtga agtgctgaat
     6781 gagggcgttg ccgatctcga agaaccagtg caccatgtag agcgagagcc cggcggccac
     6841 ggcccgccag ttccgtttct cgatctcgtt aaagtagata tacgcgacac aggcgagcag
     6901 cggaatgaca taccactgaa acgtctccgg gtttctcaaa atccccaggg ccttctgcgt
     6961 gagttccggc tggttcatgg gattcctcct gttccgatgt aattatttca tgcctatttg
     7021 tccgggcgat ggcaatcact ttttaaaccg gtccgcgctc cgttcgccgg caggcggaac
     7081 atccagcgct tccaccaccc tcctgagccg atcgtaatgg ctgcccttcc aatagacgcg
     7141 cccgcagccg tcgcaccggt ggaactcgcg gcaccaggcg cgcacgcccg gcgggacggc
     7201 gtccagggcg gggctgcact ccggcgagtc gatcggcagg gaacgcatgc gtccattgca
     7261 ttcaatacag cgggtaaagg ggctgatgct gccgcgcaga tcgagccggt caagcacatc
     7321 tgcgacctgc gcatcgatgc gggtcgcgcg cacgaacacc ccccgttcga cgatccggcg
     7381 catcaggagg cgccggtccc gcgtaagcag gatgcgccgc tcgcgcgtag agatctccgc
     7441 gagctcggcg tcgccgcggt tttctcggta gtcgacgtcg aagcccagca ggcgcaaccg
     7501 ccgcgcgagc ttccggagat ggacgtccag cacgaagcgc gtatcgcgca acggcgctgt
     7561 ccgcaggcgc gtcaccggca ggatgtccag cgattcgaac acgggataca cgctgatccg
     7621 gtcgccatcg gccacgatgc gcgagaaatc gaccgacgcg ccgttcacca ggacgaggtc
     7681 cacctcgccg tgcggcacgc ccagcgactc gatgaggtcc ttcacggagc gtcgccccgg
     7741 aaacgcgacg tcgacgtcgc gcttccgctt gccgcgcggg agaaagtcgt tcagctcttc
     7801 gtagaatcgt atcgtgatcc ggtacatgga ttgccccggg ccgagcgtat catgcggccg
     7861 gtgattatgc aaacggaaaa cgacccggcg atcgcttcgc gtcgtcggcg gcgacggagt
     7921 cggtcaaatc aagcatttcc tttttttggc gcgacatccg gaacggatgg gcgtatacta
     7981 gcgttgatgc acattagaca tcgagggata cgacaaacgt gcgattgaac gctaatccca
     8041 gtcggaggtc gaaggacatg ccatccgcag aaagaggatc gcggctccat gatggaaaac
     8101 gagccgaaac gcgcccgacg gcgctcatca cgggcggctc gtcggggatc gggaaggagc
     8161 tcgcgcggtt gttccaccgg gccgggtacc gcgtgctgat cgcggcgctc gacaggaccg
     8221 agctcgcgca aacgaaacgg gagctgctcg cggcggggaa cgatacgcgg atcgacacgc
     8281 tgacgctgga cctgtcgcgg cagggcgccg ccgcgcggat cgtgcgatgg acccgatcgc
     8341 tcggcgcgga ggtcgatgtc ctcgtcaata acgccggctt tggcctgtgg ggacaggcgg
     8401 tcgagctgcc cctgcccgag gtgcgctcga tgctcgcggt caacatcact gcggccacgg
     8461 agctgtgcca tgccatcggc ggcctcatga aggcgcgcgg ccgcggatcg atactcaacg
     8521 tcgcctcgac cgcgtccttc cagccggtgc cctacatggc cgcgtacgcc gcgagcaaat
     8581 gctacctcgc cagcttcacc gcagcgctgg ccgcggagct gcgcccgttc ggcgtcgccg
     8641 tgagcatcct gcatcccggc accacccgga cgaatttcct gtcgcgcgcg gggatcaatc
     8701 ccgggagccg ggcatcgctt gggggcctcg cccatgccat cgccatggac cccgccgacg
     8761 tcgcggccgc tggtttcgcc gccgtgctgc atggcagacg aagggcgatc cccggcgcgc
     8821 tcaaccgcct gcagttcctg gcgacgagac ttctcccggg gttcgcgatc atggcaatgg
     8881 cgaaaaaaat cttcaagaaa ccgtgactga aacagcagca acatgcttga ctttcacgga
     8941 cgagccggtc tactataggc ttgttccgca cgccggagcg ggatatgcgc aggaggtcac
     9001 ccacgacatg cagattacct ataaatgtag aaacggccac aagttcaaaa aggaggaggc
     9061 cccgaacgtg acgtgctcga gctgcggcga accggcggag gcggtgaagt ggaacaccgt
     9121 cgagagctac gcgattcccg agaagggcct tggcgtcatg gacgagatca actcggtcat
     9181 caaggagtac agaaagaaat aagaaacgat cgcgacgtga cggtgatcac gccctagcgg
     9241 gcgcaggagc gccagcaggc gcgtcgcgtc gcatagcact gcttcgcgca tttcttcttt
     9301 gccgcgcccg gcgtgacctc cgcggatttt ttcttcagct cgtccgggtc cacggcccgc
     9361 tcgcagtcct tgaggcacct cccgagcgcg gcctcgcact cgctctggca ggggttctcg
     9421 gcgcgaagaa cgccctcgcc gctcatgacg aggagtcccg ccgccgcgag gatcatcaca
     9481 aatcgtttca ttgctccaca cctcgttgct tggacgcgcc atagcgggcg atcccctcgc
     9541 gcaccatgcc ctcggggatg ccgaccgtct ccgcgatctc cggttccgcg attccatcga
     9601 gggacaatcg atagcacagg tgccccagcg attcgccgat ctccacgata tagccgtcgg
     9661 gatcgtagac gcgcagcacc cgctgccgcc acggctgctc ccgcgttttg tgcaggaatc
     9721 ggaccgcctg cccgtcaagg cgcttctcca tcgcctcgac atcgtcatac tcgaaataga
     9781 gctccacgtc gttgcgcccg cgcgccacct cgagatcgcc gatgagtccc cggtaatgcg
     9841 acttccggtg gatggcgaaa tcgcccgcga aggtgacatt gtcgccgaaa tcgaatttca
     9901 cggtttgccc caggaccgtc tcgtagaagg cccgcgcgcg ggcgatgtcc tcgaccacca
     9961 ggagcgtgca tacgaatttc atggggacct ccgcggggac cccggcggca tgcatccgga
    10021 tccccgttag cactctattc cgccgcggca tgaaatgcaa gcgggaaacg ggctcgccga
    10081 tcggcgctgt attcgcgtac accgtttcac gggtattcct gtagagcggg atcgcccgtc
    10141 atagcgcgga gcgatagcgg cgaagctatc cggtgccttc atcgtggcat cgcgcttgcg
    10201 gcacatgttt gtgtagtgct ggattcgcgc tgaggaccga ctttcttcag tcataatgaa
    10261 atcgagttga acgacaacaa aatttatcga tcgtaaacaa aggaaaaacc tcccggcttc
    10321 ttgacatttc ctccgctctc agtatcatta atactggatc cgggaaatcc gggctattta
    10381 gcggtctcac tggtacttcg gcgtaaacgg cacaaaccga gaaaaaccga ttcgccgatc
    10441 acaatgcatc gacccagcgc gtgtagagcg ttgtgtcggt gtccggaaac gctcttcaga
    10501 gcgcgcacaa gcggaagttg agggtatggg ctttccgact gtcatgtgtc gtcatcaatc
    10561 ggccccacgg ggcatcgatg tatctctgca tgcgcatcgt ccggcgtcct cccgccggca
    10621 cgggttccgt catgaacaat gacacggaaa agctccagca gcaggagatc gaccggctga
    10681 acaggcgcat cgccgagctc gagatcataa tcaagaccac cgacgagctc gaatacagct
    10741 acatacagac gcacgaccgc tatcgcacca tcctggagga aatgaacgag gagtatttcg
    10801 aaacggacct cgaggggaat ttcaccttcc tgaacgacag caccgtgcgg accctgggat
    10861 atgcgaagga cgagcttgtg ggcagcaatt acggcattct cgtccacccg atggagcgcg
    10921 accgcatctt ctcgatcttc ggaaccatct atgcaacggg cgccggcctg aaggatttca
    10981 cgtgggcgtg catcgacaaa ctcggcaggg aacacatcat gcgggcctcg gtcgtgcccc
    11041 tgaaggacca ccacggcgcg accatcggct tcaagggcat cgcccaggac atcaccgaaa
    11101 agatcgaggc ggaacggaag ctcaaggagg aggaagaact ctatcggacc atcttcaaca
    11161 cgacgagcaa tcccacgatc atcatcgacg aggacaccac gatcctgctg gtgaaccgcg
    11221 agttcgagcg attgacggga ctcccggcgg cggacatcga agggaagcgg cgttggacgg
    11281 aattcgtcgt gacggatgac cgcgaacgca tgctcgccca tcatcgcgat cgacggacgg
    11341 acggcgggaa cgcgcccagc aactacgaat tccgcttctg cggcgcgacg ggcgcgctcc
    11401 gggacatcta cctcagcata tcgctcatcc cgggcacgat gaaggccgtc gcatcgctcc
    11461 tcgacatcac cgagcggaag caggccgaca tcgagctgcg ccgttccgaa aagcgcttcc
    11521 gcggcatctt cgagaacgcc gtcgagggca tcttccaagt gacccccgac gggaggctgg
    11581 tgaccgccaa ccccgcgctg gcgacgatac tggggttcga cgatcccggg tcggcgatcg
    11641 ccgcgctcgc cgatttcggc ggttccgtgt tcgccgaccc gggcggcttc gacgatttcg
    11701 ggcaacgcct cggacgggag cacgccatca aggcctacga gacccgtttc cgccgccgcg
    11761 acggcgccgt catcgacgtc atcatcaacg cccacacggt gcgcgacggg cagcgcgccg
    11821 tggcgatgta cgaagggatg ctccaggacg tcaccagcgt caagctggcc gagaagctgc
    11881 ggctgtcgac cgaggcggcg gaggcggcga accgctcgaa gagcgaattc ctcgccaaca
    11941 tgagccacga gatccgcacg ccgatgaacg ccatcctggg cttcgccgaa ctgctggaag
    12001 gcaagatcga cgatccggtc ctgcggcagt acatcgcctc catacgctcg agcgggacca
    12061 tgctcctggg cctcatcaac gacatcctcg acctctccaa aatagaggcg gggaagttcg
    12121 agatccgcgc ccggccggtc gatccgcacg cggttttctc cgagatacgg cagctgttcg
    12181 ccgcccgcat cgccgagcgc ggcgtcgcct tcatcctcga catcgacccc ggcctgcccc
    12241 cgtcgctcgt gctcgacgag gtccgcatgc ggcagatcct cttcaacctc gtcggaaacg
    12301 cgctcaaatt caccgaccgc gggcggatca cgcttaccgc ctgccggctg gacaaccggg
    12361 acgacgccga cgccatcgac ctgctcgtct cggtcgccga caccgggatc ggcatcccgc
    12421 ccgagcagca cgacctcatc ttcgagtcgt tccggcaggc cgagggcggc cgggcctccg
    12481 cgatcgccgg gagcggcctc gggctcgcca tcaccaagcg gctggtcgag atgatgaacg
    12541 gcgccatcac ggtcgagagc gaaccggggg acgggagcgt gttcaccgtc gtcctccgcg
    12601 gcgtcgcggt cgcggacgag gcggacgggg ccgatacggc cgcgcccgac gcgatgccgc
    12661 agtacgcatt cgaccgcgcc cgcgcgctca tcgtcgacga catcgaaacc aaccggatgc
    12721 tgctgcgcgg ctatctcgcg ttgtacgaca tcgactgcgc ggagtccgca gacgggcgtg
    12781 aggcgatgga gcaatcgcgc cggaaccctc ccgacgtcgt cctgatggac atgaagatgc
    12841 cggtcatgag cggctacgac gccatccggg ccatgaaggc ggacccggcc ctgcgcgaca
    12901 tccccgtcat cgccgtgtcg gcctccacga cgttgcgcga cgtcgagatc atcgagcaga
    12961 ccggctgcga cgcgttcctc cgcaagccgg tgtcgcgcgc cgacgtcatc gccgccatcg
    13021 cgcggttcct gccccaccgg atcgtcgagc cgaccgatcg gcggccggcg gaggagtcgg
    13081 cggtcccgct catgcgcgcg atggacgacg aggcgcggat gcggctgccg gagctcatcg
    13141 aacggctcga gggcgactgc atggcccgct ggctgcgcat ccagaagacc ttcatcatca
    13201 acgaggtgaa ggagttcgcg cggaccatgt cggcgctcgg atcggcctat gcggtcccgc
    13261 cgctcgacga ctggggcgcg gcgctcatgc gcgaggccgc gagcttcgac atgaatgcgc
    13321 tgccgtccac gctgaaacga tatgaagagc tgctcgtcgc gatcaagggc gcgacggaag
    13381 gcccggcgat accgacgaac gaccgaccgc aggagtgacc gccatggaac caatgaccgc
    13441 atcgagtctc aacgccaagc cgctcatcct gatcgtcgac gacgccccca agaacctcca
    13501 ggtgctcggc gccatgctcg acgagcgatc gtacgaggtc gccgtcgcgt ccaatggagc
    13561 gcaggcgatc tcgctcctct cggagatccg gcccgacctc atcctgctgg acgtgatgat
    13621 gccggtcatg gacggattcc agacctgccg catgatcaag gagtctcccg agacgcgcga
    13681 catccccgtc atcttcctga cggcgcgcga cgagaccgag agcatcatca ccggcttccg
    13741 cctgggcgcc gtcgattaca tcaccaagcc gttcagctcc tacgagcttt tggcgcgggt
    13801 ggacgcgcac gtcgagctca agcggtgccg cgacaacatc gtcgagtgca acaaccgcct
    13861 aaacctggag attgaatacc gcaaacagat cgcgcacgaa aacgaagcgc tcctgaacga
    13921 cctcctgaac aacaaccgcc ggctcgagaa cctcgccgtc accgacagcc tcacgggtct
    13981 gtacaaccac aacgtgatca tcgaccgcct ggaaggcgag gtgaatatgg cgcaccggta
    14041 ccaaacgccc ctgtcggtca tcatgttcga cgtcgaccac ttcaagcgaa tcaacgacac
    14101 ctacgggcac ctgatcggcg acgaggtgct ggtgcggatc tcgtcggtca tgcgcaaccg
    14161 actccgccgg gtcgacgtct ccgggcgata cggcggcgag gagttcctgg tcatcctgcc
    14221 ccacacggag atgaagaacg gctacttcat cgccgacaag ctgcgcaaag cggtcaagga
    14281 ccagcaatgg agccggagcg ggctcgaggt caccatcagc ggcggcatcg ccgaattcca
    14341 ggacgagaac ccgcgcgatc tgatcgaaaa ggccgaccgc ttgctctaca aggcgaagga
    14401 acatggcaga aaccgcatcg aattcaactg acgcacgcac gccgctcgtc ctgatcgtgg
    14461 acgacaatcc cggaaacctc caggtgctgg gaagcgtcct gtccggcgcc gagatcgatg
    14521 tcgcgatcgc gaccggcggc cgcgaggcgc tcgcgtcggt ggaggaggcc ctgcccgatc
    14581 tcatcctgct cgacctcatg atgcccggcc tggacgggct ccaggtgtgc cgcgcgctca
    14641 aggagtccga gcggacctgc gacgtgcccg tgatgttcct caccgcgcgc cacgagacgg
    14701 acgacgtgct gcaggggttc caggccggtg cggtcgatta cgtgtgcaag ccgttcaacg
    14761 cccccgagct cctggcccgc gtgcggacgc acctcgagct caggctcgtg aaacagcgcc
    14821 agcgggagat catacgggag ctcgaacgcg cgcaggcgga cctgaagaag aaatacgaca
    14881 tcatctgcag cgatctggcg ctggcgaaga aaatacaagg gcggatgttt cccgacgaca
    14941 tcgactcgat catcggctat gcattcgaca tccgctacct gccggtcgag gacgtcgggg
    15001 gcgattactt cgacatctgc cggctgaaca acggcgtcgt ccggatcatg ctggccgacg
    15061 cgaccgggca cggcatccag gcggcgctga tcaccatgct catcaaaagc gaatacgaaa
    15121 agctcaagga aatcatccga aaaccctccg aggtcctcga gctcctcaac aatgtgtttc
    15181 tgcgcaccta ccggacgatg gggatactct tcaccggaac gatactcgac atccatcccg
    15241 ggcgcgaccg gctggcctac tcctcggcgg gacagacccc cgccctcctc ctgcacgaga
    15301 accgcatcga gccgctcggc gagaccgggc ggatgatcgg cgtgctcgag ggcgaacggt
    15361 atcggaccgt cgaacacgac ctgtcggcgg cgacgcgcat gctcttgtat tccgacggcc
    15421 tgtacgagca gttcaatgcg aagggcgaga tgttcgggga cgagcggatg atcgcgtaca
    15481 tcgattcgat acggacgcag ccgaccggcg cgatcatcga ccggctgctc gcggaggtga
    15541 tgcgcttccg gggcacgagc gagcggatca cccgcaacga cgacatcacc gtgatcggcg
    15601 tatcgccccg ctgaggcgac ccggcgcgcg gatcacgcgc cgtgcggcgg cacccgcttc
    15661 cattcgcccc gctgcgcgat cgtttcgaga tgctgccggt tgttgacgta attctcgaaa
    15721 aacccgcagc tgacgcagag atagcaatcg aaggtggtgc tgctcgtaaa gaatcccccc
    15781 ttcttgatat attgacactc ggcgctaccg atcccgtctt ccagcagata cacggtcgcc
    15841 gaaccgcatt tcgggcaggt gccgcgtttc atcctcgccc cccttctgta tgatagaatt
    15901 tcgttccagc cgaatccctc gtccgcgcac gcgccgctgt atggaggcgt cgcgccgacg
    15961 gccgcctcgg gcggaaacat cattgacacg aaacggcccg ccggtgtgtg cttcgttttt
    16021 cgatatcgcg cgaccacgga gacgcccatg gactgctcag catgcggggg actgctcatc
    16081 gaggggaaga gctgctacac ggccagcaag gaccattaca gcctcgtcct cgaggacatc
    16141 cccgccttcc agtgcacgcg ctgcggcagg gtcctgttcg cggccgagac cgtcgataaa
    16201 atcgaaacga tggtcaagcg gatcgagcgc gagagccgcg agatcgtctc cggcaagccc
    16261 accacgaatc tgtacgactt ctagcgcgcc ctccggcgcg ccatcacccc gccccgcccg
    16321 ccttttcgat tttcgcccac gagtcgcgaa gctgcaccgt ccggttgaag acgagcctcg
    16381 cctcagtgga atcggggtcc acgcagaaat agccgtggcg cagaaactgg aagatggcgc
    16441 ccggcgcggc ccccttcacg ctcggctcga tgaagatgcc gggaagcgtt tcgagcgact
    16501 gcgggttgat cgcgtccagg aagttctcgc cctcggcgac atcgtccggg ttctctttcg
    16561 tgaagagcgg attgtacagg cgcgcctccg ccgtgagacc gtgcgcggcc gacacccagt
    16621 gcagcgtccc cttcaccttc ttccccgagt tgtcggcgcc gcttttcgtc gtcggatcgt
    16681 acgtgcagcg aagctccacc acgttccccg cggcgtcctt gatcaccttc tcgcatttga
    16741 tgatgtaggc gctcttgagc cgcacctcgg cgccggggga gaggcgatga tacttcttgg
    16801 gcgggtcctc gcggaaatcg tcgcgctcga catagagcgt ccgcgagaag gggatcttcc
    16861 gcgtcccggc gctcgcgttc tcgggattgt tctcggcgtc gagctcctcg agggcatcct
    16921 cgggatagtt ctcgatcacc accttcagcg ggtcgagcac cgccatcacg cggtgcgccc
    16981 gccggttgag gtcgtcgcgc acgcaatgct cgaggtatga gaattccacg gtgctgtcgg
    17041 ccttcgcgac gccgatgcgc tcggcgaagt cgcggatggc ctcgggcgta tagccgcgcc
    17101 gccgcatgcc cgcgatggtg ggcatgcgcg gatcgtccca gccggcgacg cgcccctcct
    17161 tgacgagctg caggagcttg cgcttgctca tcaccgtgta ggagaggttg agccgcgcga
    17221 actcgatctg ctgcggacgg ttgggcgtgt cgagcgccgc gaggaaccag tcgtagagcg
    17281 cccggttgtt ctcgaactcc agcgtgcata tggaatgggt gatcccctcg agcgcgtccg
    17341 agaggcagtg cgtgaaatcg tacatggggt agatgcacca catgtcgccg gtgcgatggt
    17401 gcgtcgcgcg caggatgcgg tacatcaccg gatcgcgcat aacgataaag ctggcggcca
    17461 tgtcgatctt cgcgcgcagc gtcttggagc cgtcggggaa ctcgccgttt ttcatgcgcg
    17521 cgaagaggtc gaggttctcc tcgaccgacc ggtcgcgata ggggctgggc cgcccgggct
    17581 ccttgagcgt gccgcggtac
//
LOCUS       DAORHF010000047        17467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2354_length_17467_cov_6.548989, whole genome shotgun sequence.
ACCESSION   DAORHF010000047 DAORHF010000000
VERSION     DAORHF010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17467
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2354_length_17467_cov_6.548989"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="PKJ16_06070"
     CDS             complement(<1..1979)
                     /locus_tag="PKJ16_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46590.1"
                     /translation="MIRHGTLLLKIIISAGAVCSLMVAFSKTRRDPQSFTEALQDYLT
                     THATGETTEVYLHDPRGDELRAQEGRTDSLRYLHGDWQKYVTREKYRMLLDDMHEDAF
                     KIQEEGDVNITTYGSMKYTMLYGKSRFKNDDMQQFDTDKAVSRVIQDGFTNKQEVQLH
                     MEGRVGKRLTVFIDHDSRKTNNQYYMQYRAVNEDEVIREINAGQIDIKMNNSKYAVYD
                     DNTAKALGIDLTLRKKKLTVKAFGSMIQGVTEVERFRGTSAPGSIKLSEFQYMRNRYF
                     QLEPFKRYDGRTTPPVLADTPYDTMVTFTSRPADPSKYAPYLVNIDPGGFELYMDDMN
                     PLNNYNATNLSIAGYDHGWYHKLVPGADYIINYSTGMITFLKTVPENARIYAVYRMAG
                     GTATSDPTARTDVVAGKLFVFLKYGTAINEDLNRNYQRDANEPDKNNDGLLNLDIYEV
                     RSFYRIGESQITETNFNLSFYQDNALMTSTARAALGRYQVDFQGGVISFVLREPFREN
                     LRAAGGNTEGVYCEAQSPGAYLYSKYNIRADYFRDARTYQLKHGNIIPESVRVKVNGR
                     ELSPSLYVVDMTSGLFQFNNPGNPVISQETEIEIKYEYMPFGGHSQQFVGGVRADYKV
                     NRHLSVGGTVLMSRDSSRKVIPTVGAEPEQKMVFEG"
     gene            complement(2171..3166)
                     /locus_tag="PKJ16_06075"
     CDS             complement(2171..3166)
                     /locus_tag="PKJ16_06075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV46591.1"
                     /translation="MRYRIKNYHGKMKLKYGEKLQVRKKKMGKWWDRFREKGHEKMTI
                     MFIPHNEKKIFTFQISKFFILFFVLLFMVVLATSSYAVIKNASIKREERRLLSNYKDV
                     RSILLRFEKSTNSIQRHMDDMKPEIEELYALASGNEGRVRGIWKSIELPAPAGDEAKL
                     RGILPDEIYALKDLQKDIVATTNTVKVVKNFIDVRTKVINETPSIVPNVGHITSLFGW
                     RRSPFGFGRDWHAGIDIAGPHGSDIRATAPGKVVVAGWSGGYGYLVHIRHKYGFETLY
                     GHCSEILVKEGDAVKKGQVVAKVGQTGSATGNHCHYEVRLGNIAINPYPYMRKVW"
     gene            complement(3262..4596)
                     /gene="radA"
                     /locus_tag="PKJ16_06080"
     CDS             complement(3262..4596)
                     /gene="radA"
                     /locus_tag="PKJ16_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HNV46592.1"
                     /translation="MAKKEPTYLCNSCGREFVKWQGRCPQCGDWNTIIERPAHAPAER
                     RRALPQATALSAVSYAGLERLRTGLGEFDLVCGGGIVPGSVVLVGGEPGIGKSTLALQ
                     IAASLPSLYVSGEESPVQIRLRAERLGIAVDGIQLTTATDADEIAALAEAQRPACILV
                     DSIQTVTVSSAPGIAGSVSQIRESAQRLVECAKSIGIPLVLIGHITKDGGIAGPKLLE
                     HIVDTVLYFEGDFTREYRVLRAFKNRFGSVNEIGLFAMTAQGLKEVDDKNSLFTNPYA
                     AAAPGSAVAAAVTGTRTVLFEVQSLVTASAFSNPRRMADGFDLNRLVLIVAVLEKFAL
                     LKLGSFDVFVNIAGGFSVDDTSADLAVAAAIASSLKNRPLDARMAFLAEISLSGELRP
                     VAQCRRRVQELERSGFRAAVLSKKDAASMADLALSLELVGLETIAEALDRVF"
     gene            complement(4640..5206)
                     /locus_tag="PKJ16_06085"
     CDS             complement(4640..5206)
                     /locus_tag="PKJ16_06085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV46593.1"
                     /translation="MRAVRILSWGACALFLLAAQATAAHAGAVSGRLGAWDFINDDVR
                     NRYRVAPAAMIAYTFMDKSLMSVEIASGLVYTSINYRTYRYNFYLVPTTVSYLVNITG
                     ERLPVIPYFGAGLGCYYKRDDNTTLRRSYSYTTYGYSFIAGFKLPLETGLFFYADLRY
                     HLIQAHEPTEANLSGVSTALGAGYSFEW"
     gene            complement(5263..6744)
                     /gene="lysS"
                     /locus_tag="PKJ16_06090"
     CDS             complement(5263..6744)
                     /gene="lysS"
                     /locus_tag="PKJ16_06090"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HNV46594.1"
                     /translation="MNAETSDLIAQRIEKVNALRQKGINPYPVRFARTALAKEIKDTF
                     VDGEERVVAIGGRIRTRRLMGKASFANIEDLSGSIQIYAKRDDLGEEAYERYTGLDLG
                     DIIGVEGKTFRTKTGEISVHVTSCALLAKCINPLPVVKEKDGVLFDEFADKELRYRQR
                     YVDLIVTPKSRQDFILRSRIIAGIRSYLAERGFLEVETPMMQAIPGGAAARPFITHHN
                     ALDIDLYLRIAPELYLKRLIVGGFEKVFELNRNFRNEGISTKHNPEFTMMELYQAYAD
                     YHDMMAITEGMISTLAQDLLGTMRIDYQGSTVDLTPPWERITFVNIIKKYTGIDFETI
                     TTVEAARAAAESVGLHPDTDLSIWKIANEIFEERVEDKLIQPVFVMDYPKELSPLAKS
                     REDNSALVERFEPYIVGREIGNAFSELNDPFDQRARFEEQVRLREAGDDEAQMMDTDY
                     INALEYGMPPAGGLGIGIDRLVMLFVNTASIKDTILFPLLRPE"
     gene            complement(6897..7448)
                     /locus_tag="PKJ16_06095"
     CDS             complement(6897..7448)
                     /locus_tag="PKJ16_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46595.1"
                     /translation="MKKLTVKQIDKAIAGFVPIDGDRYNDLLADLVARQPVIGDYVFS
                     VDKKHLNEDEHSFMMNWTVFIWRIMSEYYPDIPVVSESTIEMMREELSVVMEDAANAA
                     APSEVHRRFIKSCRQENLFMLIINALMSEPTDASRALAVREKNKPDILMHLRIIIDAL
                     DHAKELGDDDCVDADGSTHECCS"
     gene            7574..8710
                     /locus_tag="PKJ16_06100"
     CDS             7574..8710
                     /locus_tag="PKJ16_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46596.1"
                     /translation="MNHEFELGPIRPPSEAQSILLRVTRNCPWNQCTFCHTYRDERFS
                     RRALDEVLRDIDSIHAIAQRIREAAAAPGANGRITEAVVATAMASDPTPAHYYRQVAF
                     WMLQGMKSLFLQDANSLILATGDLVAILARANERFPGLERITTYARAKTVAKKSLAEM
                     KELRAAGLSRIHIGMESGSDRILELIKKGVTAHEQIVAGRNAIEAGFDLSEYYMPGVG
                     GRDLMEENAIESARVVNAINPTFVRIRSTVPIPGTGLYDCMSEGAWRVTTEVERVREL
                     RLFIERLEGVTSRLLSDHIMNLLEDVEGALPDDKDAMLATIDSFLGMADDDRESFIIG
                     RRLGRYRGVKDFYRDPEIESVKREIKSRYASVDEAILEILKNYI"
     gene            8730..9614
                     /locus_tag="PKJ16_06105"
     CDS             8730..9614
                     /locus_tag="PKJ16_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46597.1"
                     /translation="MQPILDIVDLKKYFPEVKAVDGVGFSLAPGTCFGLLGPNGAGKT
                     TAVEVIEGVLAPTAGEILYKGAPRGATFKNEVGIQFQNTELPQYLTVGETLRTFRGLY
                     DRRADLPWLVETCRLDEIIDRDNRRISGGQKQRLLLAMALANDPELLFLDEPTTGLDP
                     QARRHLWDIVRDITARGTTIVLTTHYMEEAEILCDVIGIIDRGRIIAQGRPRDLVREH
                     CGGASITLRGPIDDDLLGRLPCRHSRDNGRVEIQTDDPAECLRLLMERGVDLATVSVR
                     PQNLEDLFLTLTGHELRA"
     gene            9611..10639
                     /locus_tag="PKJ16_06110"
     CDS             9611..10639
                     /locus_tag="PKJ16_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV46598.1"
                     /translation="MTMAPLRRIWILFIARNREFLRDRSSLAWNILFPVLVIIGFGLL
                     FDANGQDRYKVGLIGPPGGAVSERLGKARYLSIIPFESERAAMAKLLHHRIDLLVDPA
                     RGVYWTSSTSPRSYVAERIALGAPDPRDSGLRREVVAGREVPYIEWLFPGILGMNMMF
                     SALFGVGYVVVRYRKNGVLKRMSVTPLSPWEFLTAQIVSRMFLILLTTSIVFAGVALM
                     KGFAVRGSPLSLALVFALGGFSMIALALLIAARSGSEEFAGGVLNIISWPMMFLSEVW
                     FSLEGALSWVQSAALLFPLTHMIRAARMIMNDGAGLFDVRWHLAALALMSLVFLAAGS
                     LLFKWQKT"
     gene            10736..12715
                     /locus_tag="PKJ16_06115"
     CDS             10736..12715
                     /locus_tag="PKJ16_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV46599.1"
                     /translation="MEYSLFKDFVVIFVLAALVIFICQRLRVPTIIGFLVTGVIAGPN
                     GLALVRAAKDVDAVADVGVVLLLFAIGIEFSIRRLTDIKRAVLIGGAVQIIATAIGVM
                     GFYRLPGGSTAGLAFVGIIIAMSSPALVLKKLSDRSEINTPHGQAALGILFFQGMCLI
                     PAILLAPLFARGALGSGIMPYLVAGKIVGIMLVVIVGMKWVVPFVLDLAARFKSREFF
                     MMVIIAVCLLMAWAAHQAGLPAGLAAFLAGLVLSETEYGLQAFPGMKPLRAVCAGLFF
                     VTIGMLLNPAIFVQRPGTIITLTLGVVALKIAAGTVAVVALRYPLQNAVLAGLALFQV
                     GEFAFVLSRYGVKYNIFTSQGFQIFLAISAITMAVTPFAMMLAPALGRLIGRLPLPRR
                     LVDGAGGDTGAALPDLSDHLVVIGHGLTGKNLARAARTAGIPYAVIDANLDTVRSARA
                     QGEPAVFGDASHAIVLERARIRTAKVVALTFDDFVTTRRVAEQARALNPLCRIIARSH
                     HFDHIQPLREHGVSRVIPEDFEISVLLFASVLSEYQIPRRTVETIVNEVRADEYGVFR
                     NLSRKEIDPDAAVAPEPPALSEVAVTEESGLAGRSMRQLPVGKKQRLHLLALCRDGKI
                     TAHPGADTKFAAGDILYLVSEDGRRVGPSDLRIEK"
     gene            12851..13570
                     /locus_tag="PKJ16_06120"
     CDS             12851..13570
                     /locus_tag="PKJ16_06120"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HNV46600.1"
                     /translation="MSSYVRETTLEKMIQKQVKTWESSKNVLIEKPEETRPFVTISRE
                     YGCMATPIAAAIAERLNRAEGSDAWQAYDKGLINKIVENTGVSETLIETIDTKKREEM
                     NELLRTMMTDYPPQVTVYKKLVRTIRGISIHGRAIVVGRAGVVITRDLKYGLHLQFVA
                     PLSYRIHKIMEVRGIKDKLEAERLVETKDRERHDFLTQYVKFESTSPASYDLTINIAR
                     FTDAEIAETVEGLLRAKKFIK"
     gene            complement(13626..15836)
                     /gene="katG"
                     /locus_tag="PKJ16_06125"
     CDS             complement(13626..15836)
                     /gene="katG"
                     /locus_tag="PKJ16_06125"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HNV46601.1"
                     /translation="MSDQKKEGQCPVTGAMGRAAGGRGTSNRDWWPNQLNLGVLHQHT
                     RVSNPMDANFNYAEEFKTLDLAAVKKDLYALMTDSQEWWPADWGHYGGLMIRMAWHSA
                     GTYRSSDGRGGGGTGNQRFAPLNSWPDNVNLDKARRLLWPVKKKYGNKISWADLMILA
                     GNCALESMGFKTFGFGGGRVDIWEPEEDVYWGTEDTWLGDKRYSGDRELENPLAAVQM
                     GLIYVNPEGPNGQPVPIESAKDVRVTFARMGMNDEETVALVAGGHTFGKAHGAGDAKH
                     VGPEPEAAPIEEQGLGWKSSYKSGKGGDTISSGIEGAWKPNPTKWDTGYLKVLFKYEW
                     ELLKSPAGAYIWLAKDVADEDMVVDAHDPSKKQRPMMTTADLALKFDPAYEKIARRYL
                     ANPDEFADAFARAWFKLTHRDMGPRSRYLGPEVPAEELIWQDPIPAVDHTLVDEKDIA
                     ALKAQILAAGLSIAELVSTAWASASTFRGSDKRGGANGARIRLAPQKDWEVNEPTKLK
                     KVLQALEGIQAKFNKAQSGGKKVSLADLIVLGGCAAIEKAAKNAGVEVIVPFTPGRMD
                     ASQEQTDANSFAFLEPCADGFRNYLKKKYAVPAEELLVDRAQLLTLSAPEMTALVGGL
                     RVLNVNHGKSAHGVFTARPETLSNDFFVNLLDMSTAWTPSAGDADVFEGRDRATGSLK
                     WTGTRVDLIFGSNSQLRALAEVYAGDDAREKFVRDFVAAWSKVMNADRFELARS"
     gene            complement(16000..16413)
                     /locus_tag="PKJ16_06130"
     CDS             complement(16000..16413)
                     /locus_tag="PKJ16_06130"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNV46602.1"
                     /translation="MRNSGEVVRRKSRQRERIFEYIQASMDHPSAQQVYDALREEMPA
                     LSLGNVYRNIRILIEEGRIVARDFGDGIERFDAITGTHYHFVCERCKRVSDFSMPVQE
                     RLTEAAQAVSAHSIRGHTIQFFGVCRDCGKKTRRA"
     gene            complement(16759..17424)
                     /locus_tag="PKJ16_06135"
     CDS             complement(16759..17424)
                     /locus_tag="PKJ16_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46603.1"
                     /translation="MEKSLLSLVAIVVVVIVFLIMRKRGGGNVAAALAGNFSQQFCTM
                     TGLTANANDFSGSYRGYDFVVKSETGTDYAGAIQRQGWKMYPRLTVTLRAAGSSFPLT
                     VLREEHNVLVDTNQKINDLISGGGVSLPAGIPALKGKLPRVNGVYSADQAFAAKIVSD
                     PELSKLLANWFYPNITMNGDVVELKLDHEHAQNKFRERMASPGYWVEAMNICARIADI
                     AKA"
BASE COUNT         2985 a         5770 c         5389 g         3323 t
ORIGIN      
        1 ccctcgaaga ccatcttctg ctccggctcg gcgcccaccg tggggatcac cttgcgcgac
       61 gagtcgcgac tcatcagcac ggtgcccccc accgagaggt gccggttcac cttgtagtcg
      121 gcgcgcacgc cgccgacgaa ttgctgcgag tgcccgccga atggcatgta ctcgtacttg
      181 atctcgatct cggtctcctg cgatatcacc gggttgccgg gattgttgaa ctggaagagg
      241 ccggaggtca tatccaccac gtacagcgag ggcgaaagct cgcgcccgtt caccttcacc
      301 cgcacgctct ccgggataat gttcccatgc ttgagctggt aggtgcgcgc gtcgcggaag
      361 tagtccgccc ggatattgta cttcgaatac aggtacgcgc ccggcgattg cgcctcgcaa
      421 tacacgccct cggtattgcc gccggcggcg cgaaggtttt cgcggaacgg ctcgcgcagc
      481 acaaacgaga tgacgccgcc ctgaaaatcg acctggtagc gccccagcgc tgcgcgggcg
      541 gtcgaggtca tcagcgcatt gtcctgatag aacgagagat tgaaattggt ctcggtgatc
      601 tggctctcgc cgatccggta aaacgagcgc acctcgtaga tatccaggtt gagcagtccg
      661 tcgttgttct tgtcgggctc gttggcgtcg cgctggtagt tgcggttcag gtcctcgttg
      721 atcgcggtcc cgtatttcaa gaacacgaaa agcttcccgg ccaccacgtc cgtgcgcgcg
      781 gtcgggtcgc tggtcgccgt gccgccggcc atcctgtaca ccgcgtagat ccgcgcgttt
      841 tcgggaacgg tcttcaggaa ggtgatcatc cccgtgctgt agttgatgat ataatcggcg
      901 ccgggaacga gcttatgata ccacccgtgg tcgtatccgg cgatcgacag gttggtcgca
      961 ttgtagttgt tgagcgggtt catgtcgtcc atgtagagtt cgaatccccc ggggtcgatg
     1021 ttcaccaggt acggcgcgta cttcgacgga tcggccgggc gcgaggtgaa ggtcaccatc
     1081 gtgtcatacg gagtgtccgc gaggaccggc ggcgtcgtcc gaccgtcata gcgcttgaag
     1141 ggctcgagct ggaagtagcg gtttcgcatg tactggaact ccgagagctt gatgctcccc
     1201 ggcgcgctcg tcccccggaa gcgctccacc tcggtgacgc cctggatcat gctcccgaag
     1261 gccttgaccg tgagcttctt cttgcgcagc gtgaggtcga tcccgagcgc cttcgccgta
     1321 ttgtcgtcgt acacggcgta tttcgagttg ttcatcttga tgtcgatctg cccggcgttg
     1381 atctcgcgga tcacctcgtc ctcgttcacc gcgcggtact gcatgtagta ctggttgttg
     1441 gtcttccggc tgtcgtggtc gatgaacacc gtaaggcgct tccccacccg cccctccata
     1501 tggagctgca cctcctgctt gttcgtgaag ccgtcctgga tcacgcgcga gaccgccttg
     1561 tcggtgtcga actgctgcat gtcgtcgttc ttgaaccgcg acttcccgta aagcatggtg
     1621 tacttcatgg agccgtaagt ggtgatgttg acgtcgccct cctcctgtat cttgaaggcg
     1681 tcctcgtgca tgtcgtccag gagcatccgg tacttttcgc gggtcacgta tttctgccaa
     1741 tcgccgtgca gatagcggag cgaatccgtg cgcccctcct gtgcgcgcaa ctcgtccccg
     1801 cgcggatcgt gaaggtacac ctcggtcgtt tcgccggtcg cgtgggtcgt gaggtagtcc
     1861 tgcagcgcct cggtgaagct ctggggatcg cgccgcgttt tggaaaacgc gaccatgagg
     1921 gagcataccg cccccgccga tatgatgatc ttgaggagta gcgtcccgtg cctgatcatg
     1981 tgaagtatcg tatcgcgcta ccgtgaaacg cgggcctgcc acggagtagc gccctcgcgc
     2041 ggagaaaagt caagacggat atgccgcaca tgagattatt tccgcgatgc gcatacacga
     2101 ataatacggc gcgggcgcgc cgtatgtgaa cagaaatttc gcgaagcggg cgggcgggga
     2161 aaccggcgcc ctaccagacc ttacgcatat agggataggg gttgatggcg atattgccca
     2221 gacggacctc gtaatggcaa tggtttccgg tcgccgagcc ggtctggccc accttggcca
     2281 ccacctgccc cttcttgacc gcgtcgccct cttttaccag gatctcggaa caatggccat
     2341 agagcgtctc gaagccgtac ttgtggcgaa tgtgcaccag gtagccgtag ccgccgctcc
     2401 acccggcgac caccaccttg cccggcgcgg tggcgcggat gtccgagccg tgcggcccgg
     2461 cgatgtcgat ccccgcgtgc cagtcgcgcc cgaagccgaa gggcgagcgc cgccatccga
     2521 acagcgaggt gatgtgcccc acgttgggca cgatcgacgg cgtctcgttg attaccttgg
     2581 tgcgcacgtc gatgaagttt ttcaccacct tcaccgtatt ggtggtggcc acgatgtcct
     2641 tttgcaggtc cttgagggcg tagatctcgt cgggcaggat cccgcgcagc ttggcctcgt
     2701 cgcccgcggg cgccggaagc tcgatgctct tccaaatgcc gcgcaccctc ccctcgtttc
     2761 ccgaggcgag cgcgtagagc tcctcgatct ccggcttcat gtcgtccatg tgacgctgga
     2821 tgctattggt gctcttctcg aaacgcagca ggatggagcg cacgtccttg tagttggaca
     2881 gcagccgccg ctcctcgcgc ttgatgctgg cgttcttgat caccgcatag gacgaggtgg
     2941 ccagcaccac catgaacagc agcacgaaaa agaggatgaa gaacttcgat atctgaaacg
     3001 taaagatctt cttctcgttg tgggggatga acatgatggt catcttctcg tgacccttct
     3061 cgcgaaagcg gtcccaccac ttccccatct tcttcttgcg cacctgaagc ttctcaccgt
     3121 acttgagctt cattttgccg tgatagtttt tgattcgata acgcatcgtg tcgtgttcgt
     3181 tcaattgtac tgtggcgcgc caccgcgcgc gccgttgcgg tccattctcc gccgcgattt
     3241 cgcgacaccg caaccctttt tttaaaaaac gcggtccagc gcctctgcga tggtctcgag
     3301 ccccacgagc tcgagggaga gcgcgaggtc ggccatcgac gcggcgtcct ttttcgagag
     3361 gaccgcggcg cggaatcccg agcgctcgag ctcctgcacg cggcggcggc attgcgcgac
     3421 cggacgcagc tccccggaga gcgagatctc cgccagaaag gccatccgcg cgtccagcgg
     3481 gcggtttttg aggctcgatg cgatggccgc cgcgaccgcg agatcggcgg aggtgtcgtc
     3541 gacgctaaag ccccccgcga tgttcacgaa gacgtcgaag gagcccagct tgagcagcgc
     3601 gaacttctcc agcacggcga cgatgagcac cagccggttc aggtcgaagc cgtcggccat
     3661 gcggcgcgga ttggagaagg cgctcgcggt caccagcgac tggacctcga agagcacggt
     3721 gcgcgtgccc gtcacggccg ccgcgaccgc gctccccggg gcggccgccg cataggggtt
     3781 ggtgaagagg ctgttcttgt cgtccacctc cttcagcccc tgtgcggtca tcgcgaagag
     3841 cccgatctcg ttgacggagc cgaagcggtt cttgaatgcg cgcaggaccc ggtactcgcg
     3901 cgtgaaatcg ccctcgaagt agagcaccgt gtccacgatg tgctccagga gcttgggccc
     3961 ggcgatgccc ccgtccttgg tgatgtgccc gatgagaacc agcgggatgc cgatcgattt
     4021 cgcgcactcg accaggcgtt gcgcggactc gcggatctgg ctcaccgacc cggcgatgcc
     4081 cggggccgac gacaccgtga ccgtctggat ggaatcgacg aggatgcagg cgggccgctg
     4141 cgcctccgcg agcgcggcga tctcgtcggc gtcggtggcg gtggtcagct ggatgccgtc
     4201 caccgcgatg cccaaccgct cggcgcgcag gcggatctgc acgggcgact cctcgccgga
     4261 gacgtagagc gacggcagcg acgcggcgat ctgcagcgcg agcgtcgact tgccgatccc
     4321 cggctcgccc ccgacgagga ccaccgagcc gggaacgatc cccccgccgc acaccaggtc
     4381 gaactcgccg agccccgtgc gcagccgctc caggccggca taggacacgg ccgagagcgc
     4441 cgtcgcctgc gggagcgcgc gacgccgttc cgcgggcgcg tgtgccgggc gctcgatgat
     4501 ggtgttccag tcgccgcatt gcgggcaacg gccctgccac ttcacgaact ccctgccgca
     4561 ggagttgcac agataagtcg gttctttttt cgccattgat tagtatcgtc caccgcgcca
     4621 gcgccccggc ggggcgccat caccactcga agctgtaccc cgcgcccagg gcggtggata
     4681 ctccgctgag gttcgcctcc gttggctcgt gcgcctggat gaggtgatag cgcaggtcgg
     4741 cgtagaagaa cagcccggtc tccagcggga gcttgaaccc ggcgatgaag ctgtagccgt
     4801 aggtggtgta ggaataggag cggcgcagcg tggtattgtc gtcgcgcttg tagtagcacc
     4861 ccagccccgc gccgaagtag gggatcaccg gcagccgctc gccggtgatg ttcaccagat
     4921 acgacaccgt cgtggggacg agatagaagt tgtagcggta cgtgcggtaa ttgatcgagg
     4981 tgtagaccag ccccgaggcg atctcaacgc tcatgagcga cttgtccata aaggtgtagg
     5041 cgatcatggc cgcaggcgcc acgcgatagc ggttgcgcac gtcatcgttg atgaaatccc
     5101 acgcgcccag ccgtcccgag acggcgccgg catgcgcggc ggtcgcctgc gccgcgagca
     5161 gaaacagggc gcacgcgccc cacgacagaa tccgcaccgc cctcatcgcg cctccccgcg
     5221 tccgtgtttt tttgttgcgc atacccccac gcccggccgc gcctattccg gccgcaggag
     5281 cggaaaaagg atggtgtcct tgatcgacgc ggtgttcacg aagagcatca ccaggcggtc
     5341 gatgccgatg cccaggcccc cggcgggcgg catgccgtac tcgagggcgt tgatgtagtc
     5401 cgtgtccatc atctgcgcct cgtcgtcgcc cgcctcgcgc aagcgcacct gctcctcgaa
     5461 gcgcgcgcgc tggtcgaacg gatcgttgag ctccgagaag gcgttgccga tctcgcgccc
     5521 cacgatgtag ggctcgaagc gctccacgag cgccgaattg tcctcgcgcg atttcgccag
     5581 cggcgagagc tccttgggat agtccatcac gaagaccggc tggatgagct tgtcctccac
     5641 gcgctcctcg aagatctcgt tggcgatctt ccagatcgag aggtccgtgt ccgggtgcag
     5701 gcccacggac tccgccgccg cgcgcgcggc ctcgacggtc gtgatggttt cgaaatcgat
     5761 gccggtgtat tttttgatga tattgacgaa ggtgatgcgc tcccagggcg gcgtaaggtc
     5821 cacggtgctc ccctggtagt cgatgcgcat ggtccccagc aggtcctgcg cgagcgtgga
     5881 gatcatcccc tcggtgatgg ccatcatgtc gtggtaatcg gcgtacgcct gatagagctc
     5941 catcatggtg aattcgggat tgtgcttggt ggagatcccc tcgttgcgga agttgcgatt
     6001 gagctcgaag accttctcga agccgcccac gatgaggcgc ttgaggtaga gctccggcgc
     6061 gatgcggagg tagagatcga tgtcgagcgc attgtggtgc gtaatgaagg gccgcgccgc
     6121 cgcgccgccg gggatcgcct gcatcatggg cgtctccacc tcgaggaagc cgcgctcggc
     6181 gagatagctc cgtatcccgg cgatgatgcg gctgcgcagg atgaaatcct gccgcgactt
     6241 gggcgtcacg atgaggtcca cgtagcgctg ccggtagcgc agctccttgt cggcgaactc
     6301 gtcgaagaga acgccgtcct tctccttcac gacgggaagc gggttgatgc acttcgccag
     6361 gagcgcacac gacgtgacgt gcacgctgat ctcgccggtc ttcgtccgga aggtctttcc
     6421 ctccacgccg atgatatcgc cgagatccag gcccgtatag cgctcgtagg cctcctcgcc
     6481 caggtcgtcc cgcttcgcgt atatctggat gcttcccgag aggtcttcga tgttcgcgaa
     6541 cgacgccttg cccatgagcc gccgcgtgcg aatgcgcccg ccgatggcaa cgacccgctc
     6601 ttctccgtcg acgaaggtgt ccttgatctc cttcgcgagc gcggtgcgcg cgaaccggac
     6661 cgggtagggg ttgatcccct tctggcgaag ggcgttcacc ttctcgattc tctgggcgat
     6721 gagatctgat gtttcggcgt tcattccggt tcctcgttgg gggaatagtc cgcatgcagg
     6781 gacatgattt gacagtagag acaaggcatt gccatgtccc tactgtcaaa tctttttgtt
     6841 gatgatgccg cgcggggcga aagggtcccg cgccgcgccg gggagccgcg cacgtcctac
     6901 gaacagcact cgtgcgtcga tccgtcggcg tcgacgcaat cgtcgtcgcc cagctccttc
     6961 gcgtggtcga gcgcgtcgat gatgatacgc aggtgcatga ggatgtcggg cttgttcttc
     7021 tcgcgcaccg cgagcgcgcg cgacgcgtcc gtcggctcgc tcatgagcgc attgatgatc
     7081 agcatgaaga ggttttcctg ccggcaggat ttgatgaagc gccggtggac ctccgacggg
     7141 gctgcggcgt tcgcggcgtc ctccatgacc accgagagct cctcgcgcat catctcgatg
     7201 gtgctctccg acacgacggg gatgtccggg tagtactcgg acatgatgcg ccagatgaag
     7261 accgtccagt tcatcatgaa cgagtgctcg tcctcgttga ggtgcttctt gtccaccgaa
     7321 aagacgtaat cgccgatgac cggctggcgc gcgacgagat cggccaggag gtcgttgtac
     7381 cgatcgccgt cgatcggcac gaagccggcg atcgccttgt caatctgctt cacggtcagt
     7441 tttttcatcg atgccaccct ccgtatcgat gccgtggcag gagtctgccg gcgggcgcga
     7501 aaaatagcaa tacaaaaatt gattgccggc gccccgccgg ctataatgat gggcggcacg
     7561 caacgaggag cgcatgaacc acgaattcga actcggcccc atccgacccc ccagcgaggc
     7621 tcagagcatc ctcctccggg tgacccggaa ctgcccctgg aaccagtgca ccttctgcca
     7681 cacctaccgg gacgagcgct tctcgcggcg cgcgctggac gaggtgctgc gcgacatcga
     7741 cagcatccac gcgatcgcgc aacgcatccg ggaggccgcg gccgcgccgg gggccaacgg
     7801 gcgcatcacc gaggccgtcg tcgcgacggc gatggcgtcg gacccgaccc ccgcgcacta
     7861 ttaccgccag gtcgccttct ggatgctgca gggcatgaag agcctcttcc tgcaggacgc
     7921 gaattcgctc atcctcgcca ccggcgacct cgtcgcgatc ctcgcgcgcg cgaacgagcg
     7981 cttcccggga ctcgagcgca tcaccaccta tgcccgggcg aagacggtcg ccaaaaagtc
     8041 gctcgccgag atgaaggagc tgcgcgcggc cggcctctcg cgcatccaca tcggcatgga
     8101 gtccgggtcc gaccgcatcc tggagctcat caaaaaagga gtgaccgcgc acgagcagat
     8161 cgtcgcgggg cgcaacgcca tcgaggccgg gttcgacctc tcggaatact acatgcccgg
     8221 ggtgggcggg cgcgacctca tggaggaaaa cgcgatcgaa tccgcgcgcg tcgtgaacgc
     8281 catcaacccc accttcgtac gcatccgcag cacggtgccc attccgggaa cgggcctgta
     8341 cgactgcatg agcgagggcg cgtggcgggt gaccacggag gtggagcgcg tgcgggagct
     8401 gcgcctgttc atcgaacgcc tcgagggcgt caccagccgc ctgctgtccg atcacatcat
     8461 gaacctgctc gaagacgtcg agggcgcgct ccccgacgac aaggacgcga tgctggcgac
     8521 catcgattcc tttctgggca tggccgatga cgaccgcgag agtttcatca tcgggcgccg
     8581 cctggggcgt tatcgcgggg tgaaggactt ttaccgcgat cccgagatcg aatcggtcaa
     8641 gcgcgagatc aaaagccgat acgcctccgt tgacgaggcg attctggaga ttctgaagaa
     8701 ctacatttag gagcgccggc cgccgaccca tgcagcccat actcgacatc gtcgatctga
     8761 aaaaatattt ccccgaggtg aaggccgtcg acggcgtggg cttctcgctc gcgccgggga
     8821 cctgcttcgg cctgctggga cccaacgggg cgggaaagac cacggcggtc gaggtgatcg
     8881 agggcgtgct ggcgcccacc gccggcgaga tcctttacaa gggcgcgccc cgcggcgcaa
     8941 cgttcaagaa cgaggtgggc atccagttcc agaacaccga gctgccgcag tacctgacgg
     9001 tgggcgaaac cctgcggacc tttcgcgggc tctacgaccg ccgggccgac ctcccgtggc
     9061 tcgtcgagac ctgccggctc gacgagatca tcgaccggga caaccggcgc atatccggcg
     9121 ggcagaagca gcgcctgctc ctggcgatgg cgctcgcgaa cgatcctgaa ctcctttttt
     9181 tggacgagcc cacgacggga ctggacccgc aggcgcggcg gcacctgtgg gacatcgtgc
     9241 gcgacatcac cgcgcgcggg accacgatcg tgctcaccac gcattacatg gaggaggcgg
     9301 agatcctctg cgacgtcatc ggcatcatcg accgcgggcg gatcatcgcg cagggacggc
     9361 cgcgcgacct cgtgcgcgag cactgcgggg gcgcctccat caccctgcgc ggccccatcg
     9421 acgacgatct cctgggacgg ctcccctgcc ggcactcccg cgacaacggg cgcgtcgaga
     9481 tccagaccga cgatcccgcc gaatgcctgc gcctgctcat ggagcgcggc gtcgacctcg
     9541 ccacggtgag cgtccgcccg cagaacctcg aggacctatt cctcacactc accggccacg
     9601 agctgcgagc atgaccatgg caccgctgcg gcgcatctgg atcctcttca tagcgcgcaa
     9661 ccgcgagttc ctgcgcgacc gctcgtcgct ggcctggaac atcctgttcc ccgtcctggt
     9721 gatcatcggc ttcggcctcc tcttcgacgc gaacggccag gaccgataca aggtcggcct
     9781 catcggcccg ccgggcggcg cggtgtctga gcgcctcggc aaggcgcgct atcttagcat
     9841 catccccttc gagtccgagc gcgcggcgat ggcgaagctg ctccatcacc gcatcgacct
     9901 cctggtcgat cccgcgcggg gcgtgtactg gaccagcagc acctcgccgc gcagctatgt
     9961 ggccgagcgc atcgcgctgg gcgcgcccga cccgcgcgac tccggcctgc ggcgcgaggt
    10021 ggtcgcgggc cgcgaggtgc cctacatcga gtggctcttc cccggcatcc tcggcatgaa
    10081 catgatgttc agcgcgctct tcggcgtcgg ctacgtggtg gtgcgctacc gcaagaacgg
    10141 cgtcctcaag cggatgagcg tcacgccgct ttcgccgtgg gagtttctca ccgcgcagat
    10201 cgtctcccgc atgttcctca ttctgctgac cacctcgatc gtgttcgcgg gcgttgcgct
    10261 catgaagggc ttcgcggtgc gcggctcccc cctcagcctg gcgctcgtct tcgcgctggg
    10321 cggcttcagc atgatcgcgc tcgccctgct gatcgccgcg cgcagcggca gcgaggagtt
    10381 cgccggcggc gtcctcaaca tcatctcctg gcccatgatg ttcctctcgg aggtgtggtt
    10441 ctcgctcgag ggggcgctgt cctgggttca atcggccgcg ctcctctttc cgctcaccca
    10501 tatgatccgc gccgcgcgca tgatcatgaa cgacggcgcg ggcctcttcg acgtccgctg
    10561 gcacctcgcc gcgttggccc tcatgtcgct cgtcttcctg gccgccggct cgctcctgtt
    10621 caagtggcag aagacgtagc gacacgcaca aaacgcgcgg gatcgccaaa ttcaattgac
    10681 acgcgccgcc gcccagtgcg ataccgggcg cacacatcac ccgagcgagg agcgcatgga
    10741 atactcactc tttaaggatt ttgtggtcat cttcgtcctc gcggcgctgg tcatcttcat
    10801 ctgccagcgg ctgcgcgtcc ccaccatcat cggttttctg gtgacgggcg tgatcgccgg
    10861 ccccaacggg ctcgcgctcg tccgcgcggc gaaggacgtc gacgcggtgg ccgacgtggg
    10921 cgtcgtcctg ctgctgttcg cgatcggcat cgagttctcg atccggcggc tgaccgacat
    10981 caagcgggcc gtcctgatcg gcggggccgt ccagatcatc gcgaccgcca tcggggtgat
    11041 gggcttctac cgcctgccgg gcggatcgac cgcgggcctg gccttcgtgg gaatcatcat
    11101 cgcgatgagc agccccgccc tcgtcctcaa gaaactttcc gaccgctccg aaatcaacac
    11161 accgcacggg caggccgcgc tgggcatcct gtttttccag ggcatgtgcc tcatccccgc
    11221 gatcctgctt gcaccgctgt tcgcgcgcgg cgcgctcgga agcgggatca tgccatacct
    11281 ggtcgcggga aaaatcgtcg gcatcatgct cgtcgtcatc gtggggatga agtgggtggt
    11341 gcccttcgtg ctggacctgg ccgcgcgctt caagagccgg gagtttttca tgatggtcat
    11401 catcgccgtg tgcctgctca tggcgtgggc ggcccatcag gccgggctgc cggcgggact
    11461 ggccgccttc ctggcgggcc tcgtcctctc agaaaccgag tacggactgc aggccttccc
    11521 ggggatgaaa ccgcttcgcg ccgtgtgcgc ggggctcttc ttcgtgacca tcgggatgct
    11581 gctcaacccg gcgatcttcg tacagcggcc gggcaccatc atcacgctca cgctgggcgt
    11641 cgtcgcgctc aagatcgcgg cggggaccgt cgcggtcgtc gccctgcgct atccgctcca
    11701 gaacgccgtg ctggcgggcc tggcgctctt ccaggtggga gagttcgcgt tcgtcctgtc
    11761 gcgctacggc gtcaagtaca acatcttcac ctcgcagggc ttccagatat ttctcgcgat
    11821 cagcgccatc accatggccg tcacgccgtt tgcgatgatg ctcgctcccg ccctcggtcg
    11881 gctcatcggg cgcctacccc taccccgccg cctggtcgat ggcgcgggcg gcgacaccgg
    11941 cgccgcgctt cccgatctct cggaccacct ggtcgtcatc ggccacggcc tcaccggaaa
    12001 aaacctcgcg cgtgcggcgc gcaccgcagg gatcccctat gcggtcatcg acgcgaacct
    12061 ggacacggtc cgatccgcgc gcgcgcaggg cgaaccggct gttttcggcg atgcgtcgca
    12121 tgcgatcgtc ctcgaacggg cgcgcatccg gacggcgaag gtcgtcgcgc tcaccttcga
    12181 cgatttcgtg accacgcgcc gcgtcgccga gcaggcgcgc gcgctgaacc cgttgtgccg
    12241 aatcatcgcg cgaagccatc atttcgacca cattcaaccc cttcgcgaac acggcgtgag
    12301 ccgggtgatc ccggaggatt tcgagatatc ggtgctgctg ttcgcaagcg tcctgtcgga
    12361 ataccagatc ccccgccgca ccgtcgagac gatcgtgaac gaggtccgcg ccgacgagta
    12421 cggagtgttc cgcaacctgt cgcgcaagga gatcgatccg gacgccgccg tcgccccgga
    12481 gccgccggcc ctttcggagg tcgccgtcac ggaggaatcc ggccttgccg ggcgctccat
    12541 gcgccagctc cccgtcggga agaaacagcg gctccatctg ctcgcgctgt gccgcgacgg
    12601 aaaaatcacc gcacaccccg gcgccgacac gaagttcgcc gccggcgaca tcctgtactt
    12661 ggtgagcgag gacggccggc gcgtgggacc gtcagacctt cgcatcgaga aatgatcgtc
    12721 gacctgcggg acggcgatcg ggcggcataa aaaataaaat atatcgcccg gcggaaaaat
    12781 attgatttaa ttgggtgcgc ctatagcatg gcgaaaagca tgatgacagc atgcaccgcg
    12841 gagttgaacc atgagcagct acgttagaga aaccaccctc gaaaagatga tccaaaagca
    12901 ggtgaagacg tgggagagtt ccaagaacgt cctcatcgaa aagcccgagg agacgcgccc
    12961 cttcgtgacc atatcgcgcg aatacggctg catggcgacg ccgatcgccg ccgcgatcgc
    13021 cgagcgtctc aaccgcgccg agggctccga cgcctggcag gcctacgaca agggcctcat
    13081 caacaagatc gtcgagaaca cgggcgtctc cgaaacgctc atcgagacca tcgacaccaa
    13141 gaagcgcgag gagatgaacg agctgttgcg caccatgatg accgactatc ccccgcaggt
    13201 gaccgtgtac aagaagctgg tgcgcaccat ccgcggcatc tccatccacg ggcgggccat
    13261 cgtggtcggc cgcgcgggcg tcgttatcac gcgcgacctc aaatacggcc tgcacctgca
    13321 gttcgtcgcc cccttaagct accgcatcca caagattatg gaggtccgcg gcatcaagga
    13381 caagctcgag gccgagcggc tggtcgagac gaaggaccgc gagcgccacg acttcctcac
    13441 ccagtacgtg aagttcgaat ccaccagccc ggcctcgtac gatctgacca tcaacatcgc
    13501 gcgcttcacg gacgccgaga tcgccgagac ggtcgagggg ctgctgcggg cgaagaaatt
    13561 catcaagtaa cgcgcgcacc gggacgcggc atcggaggcc gcgccgcgat gcgcgttcat
    13621 tcccgctacg atcgtgcgag ctcgaaacgg tcggcgttca tcaccttgct ccaggccgcc
    13681 acgaaatcgc gcacgaactt ctcccgcgca tcatcgccgg cgtagacctc cgcaagcgcg
    13741 cgaagctgcg agttggagcc gaagatgaga tcgacgcgcg tgccggtcca cttcagcgaa
    13801 cccgtcgcgc gatcgcgtcc ctcaaacacg tccgcatcgc cagcactcgg cgtccatgcc
    13861 gtactcatgt ccagcagatt cacgaagaag tcgttgctga gcgtctccgg acgcgcggtg
    13921 aacacgccat gggcggactt cccgtggttg acgttcagga cgcgcaggcc gccgacgagg
    13981 gcggtcatct cgggagcgct cagcgttaga agctgcgccc gatcgaccag cagctcctcc
    14041 gccggcaccg cgtatttttt cttgagataa ttccggaaac cgtcggcgca cggctcgagg
    14101 aacgcgaacg agttcgcatc ggtctgctcc tgcgaggcgt ccatccgtcc cggcgtaaag
    14161 gggactatca cctcgacgcc ggcattcttc gccgccttct cgatcgcggc gcacccgccg
    14221 agaacgatca gatcggcgag cgacaccttc ttcccgcccg attgcgcttt gttgaacttc
    14281 gcctggatcc cttcgagggc ctgcagcacc ttctttagct tcgtgggctc gttgacctcc
    14341 cagtcctttt gcggcgcgag ccggatgcgc gcgccgttcg ccccgccgcg cttgtcggac
    14401 ccgcggaacg tcgatgccga cgcccaggcg gtcgaaacca gctcggcaat cgacaggccc
    14461 gcggcgagga tctgcgcctt gagagcggcg atgtccttct catcgaccag tgtatgatcg
    14521 acggccggga tcgggtcctg ccagatgagc tcctcggccg ggacctccgg gcccaggtag
    14581 cgcgagcgcg gccccatgtc gcggtgcgtc agcttgaacc atgcgcgggc gaaggcgtcg
    14641 gcgaattcgt ccgggttcgc cagatagcgc cgcgcgatct tttcgtatgc agggtcgaat
    14701 ttgagggcga ggtcggcggt ggtcatcatg gggcgctgct ttttcgacgg atcatgcgcg
    14761 tcaacgacca tatcttcgtc tgccacgtct ttcgccagcc agatataggc ccccgccgga
    14821 ctcttcagca gctcccattc atacttgaac agcaccttga gatatcccgt gtcccacttc
    14881 gtggggttcg gcttccaggc gccctcgatg ccgctcgaga tcgtgtcgcc gccctttccg
    14941 cttttataac tgctcttcca gcccaggccc tgttcctcta tgggcgccgc ctcgggctcg
    15001 ggaccgacat gcttcgcatc gccggcgcca tgcgccttgc cgaaggtgtg tccgccggcg
    15061 acgagcgcga ccgtctcctc gtcgttcatg cccatccgcg cgaaagtgac gcgcacgtct
    15121 ttcgccgatt cgatcggcac cggctgcccg ttgggcccct cgggattcac gtagatgagc
    15181 cccatctgca cggccgcaag cgggttctcg agctcgcggt cgccgctgta gcgcttgtcc
    15241 cccagccagg tgtcctccgt accccagtag acgtcctctt cgggctccca gatatccacg
    15301 cggccgccgc caaagccgaa ggtcttgaat cccatcgact ccaaggcgca gtttccggcc
    15361 agaatcatga gatcggccca ggatatcttg ttcccgtatt tcttctttac cggccagagc
    15421 aggcggcgcg ccttgtccag gttcacgttg tcgggccagc tgttgagcgg cgcgaaacgc
    15481 tggttgcccg tgccgccgcc cccgcgcccg tcggagctgc ggtacgtgcc ggcgctgtgc
    15541 caggccatgc ggatcatgag gccgccgtaa tgcccccagt cggccggcca ccattcctgc
    15601 gagtcggtca tcagcgcgta caggtccttc tttacggccg ccagatcgag cgtcttgaat
    15661 tcctctgcgt agttgaaatt ggcgtccatc gggttcgaga ccctcgtgtg ctggtgcaga
    15721 acgcccagat tcagctggtt tggccaccag tcgcggttcg acgttccgcg acctccggcg
    15781 gctctcccca tggcgcccgt taccgggcat tggccctctt ttttttgatc gctcattcgc
    15841 gtcctcccat gtgttgtatc gtgttcaatg cactctatat gctgcattcg tatcggccca
    15901 atcgaccgat cgcattcaag catcccgaag gcgcggcgac gtgcagcggc gccgggacga
    15961 ttcgtctcta cgccgcatcc gccaccggcc gtttcgtttt cacgcgcgtc gcgtcttctt
    16021 gccgcagtcc cgacacaccc cgaaaaactg gatggtgtgt ccgcgaatcg aatgggccga
    16081 cacggcctgc gccgcttcgg taagccgctc ctggacgggc atcgaaaaat cgcttacccg
    16141 cttacaccgc tcgcacacga aatgatagtg cgtcccggta atcgcgtcga agcgctcgat
    16201 gccgtccccg aagtcccgcg cgacgatccg gccctcctcg atcaaaatcc tgatgttgcg
    16261 atacacgttc cccaggctca gagccggcat ctcctcccgc agggcgtcat agacctgctg
    16321 ggccgacggg tggtccatgc tcgcctgaat atactcgaag atccgttccc gctgcctgct
    16381 ttttcttcga accacttcgc cagaattgcg catggggcac ccacggaatc gttattaata
    16441 ataattatta ttactactta aatgcgcgtt cgtcaatttt ttttgttttt tggtatgatc
    16501 cgccgcgaaa gagcgtcgcg acgcattcct cgttggcgcg atgcggtcgg tcgcgatcag
    16561 ttcaaaatac ctgatgccgc agcatgcgcc cgtttcgcga caatggcgcg cgccagacaa
    16621 ccgccgctgc gcctgccgca ccggcgccgg cgcgccgaat catccccgtt gagggcattc
    16681 gaattgcgac gggatgcggc cccggacgcc tcgatggcgg ccggaaccgc agcaatcacg
    16741 cccggcgcgc cacggacgtc acgccttcgc gatgtccgcg atcctcgcgc agatgttcat
    16801 ggcctccacc cagtaccccg gggacgccat ccgctccctg aatttgtttt gcgcgtgctc
    16861 gtggtccagc ttgagctcga cgacatcccc gttcatggtg atattgggat agaaccagtt
    16921 ggccagcagc ttcgacagtt ccgggtcgga cacgatcttc gccgcaaagg cctgatcggc
    16981 ggaatacacg ccgttcacgc ggggcagctt ccccttgagc gccgggatcc cggccggcaa
    17041 cgaaaccccg ccgccggaaa tgagatcatt gattttctga ttggtgtcca cgagcacgtt
    17101 atgctcctcg cgcagcaccg tcagcggaaa cgacgatccg gctgcgcgca gggtcaccgt
    17161 gagccgcgga tacatcttcc agccctggcg ctgtatcgca ccggcgtaat cggtcccggt
    17221 ttcggatttc acgacgaaat catatccccg gtacgatccc gagaaatcat tggcgttcgc
    17281 ggtgagcccg gtcattgtgc aaaattgctg cgagaaattt cccgccagcg cggcggcgac
    17341 gtttccgcca ccccgctttc tcataatcag aaacacgata acgaccacga caatcgcgac
    17401 gagactcaga agtgattttt ccatcgtttc ctcccatagt ggtattgtat tcgccgcgga
    17461 cggaacg
//
LOCUS       DAORHF010000048        17377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2375_length_17377_cov_5.514375, whole genome shotgun sequence.
ACCESSION   DAORHF010000048 DAORHF010000000
VERSION     DAORHF010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17377
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2375_length_17377_cov_5.514375"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PKJ16_06140"
     CDS             complement(<1..291)
                     /locus_tag="PKJ16_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46604.1"
                     /translation="MPRTIALIIAFLLLAQSVALPLGLNDPFVYPFGTIGNRQPHVLW
                     IDKYNERDREKNVRYRITVTRKSLPGGQTPVPETFLAAPQLYKKFFFGYQLPR"
     gene            complement(293..1225)
                     /locus_tag="PKJ16_06145"
     CDS             complement(293..1225)
                     /locus_tag="PKJ16_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46605.1"
                     /translation="MIDNAARIGMVRIVTGTNMSTPFNIDAAVERKVRIARAGLYGFI
                     CGLMGAMVLLPLGSILGIFTHPALSMAGSALMYAGGACFIGTLCSFFVLIFGLLLSPR
                     EHDIRDRLAPSVSGFVLIFAIGVSMFVLIVVGMMMAGAIGLFISHRAPIFAALAYFIA
                     GCAFFFLAHMYIMARGIITANEYPRWMAPLAKRFRTRDAADRFSPATDAKIVKVRMAL
                     SKAQIVLGIAIGFAFTFVTGASEYTDLLRPVIGSERAERAERVLGSTDALLLALTLFV
                     FVFTSMFVVKTATSIIDFRRDRAMGRIDETYGAR"
     gene            1342..2208
                     /locus_tag="PKJ16_06150"
     CDS             1342..2208
                     /locus_tag="PKJ16_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46606.1"
                     /translation="MTDIMPQDEIDALLAEVSGETVNSTACRIDGNDNPDMRPKLYDF
                     RRPQFMTKSAESRLRDIFATACDGIEAALGESAGIACDRGSITVDQLTCGEYVRSLMS
                     PTLFSTIRIEPLPGYVAIEVMPSLACALIDAMAGRDIRDVSLNLGFTADEAREAAAPL
                     GDIAESICRAFGESAELRSRVKAIYSNPRDAEFFGDDEMGVCVTCAAKIDYVEGLISV
                     FLPRISFGPALSLLEESAPAADVLGNRVIDVPLKTRVATLDQVRKLDAGRKLEIDTAA
                     NPTAAMRAPMEE"
     gene            2214..5117
                     /locus_tag="PKJ16_06155"
     CDS             2214..5117
                     /locus_tag="PKJ16_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46607.1"
                     /translation="MNSDFLKKLKLPLRLRLPLDLTIGDTGSLVVRRDLSEYQGNEGA
                     LYLGGMNVAEAVLRHHAGKDVYYIEIVAPRENVDDDRLLASTVRLELVFGIAEQPENM
                     PLELAHGATVPLRPLYPIKEIMKNDAAGVAEHESSSEPAPDPSEPTESSAKKGPMSME
                     EIDDLLAAISKGDAGASPSSAHAGVALSLDGLIVAKGETVLVDDRFGIRITEAVEGDD
                     RFALMERPDAGLIRGVAVLASRSIALADLPDLTEGSVITMDATALGPSVLRFDNGIVH
                     AGEILFGDAHDAGWAFRRLYSLEEITRSLMQDRMRAAAFASDDGEGDIADGATDVDEV
                     AVFIDSPMDAAGATMGNEDMSAEDANARRWAALEAAEAEMTDEHESTPEELFPDEIRQ
                     QLLEHAQSIDFMDAPSFTKENLLTLSRIIKNMCLSMGGAIDDLLSLERSVHLRLKRAR
                     VAPFSEFHVDDGLDSMPWSIELDPNLGFVFVKPGRGPLVSPEGVDKVLELMGETSADS
                     SSNISIETWVHMYLTLTICDQLIRAFEPLVAIQTRTQSFDENAETGAGFVSPVTAVPA
                     DARSALFEIAIENGDVRNAITLAMPLAGLAPIVPILEKEYLDGTMEVPGEIRSLFAGR
                     DDDVADEQDPSECDTYPEDMPWERRLPIACYLMDHAAVREFLDQGGDPNIQDEDGETP
                     LETLAANWFDADPGVELTRVKEMHGIRMERIVGPDDARECAYLLLDAGARVTKLAGER
                     AKEGSSIDGAPKRELIRLLRECLGARKDGTPFERWWEIPARTDSREASETKVMSEDQI
                     DDLLASISSESTGPFAHAEELFAEGEYLEAIDAYLQAMKEAGENAAAHNNIGLSYLNL
                     GEYEKALKYFKKSLQVDPDYATALANQACAYKRMGDIDRAIAVSTEALARGETGDILY
                     NLACYTAINGDAKTAMAYLKRAIDHDERFREMAADDDDFAALEDSKDFRKLVG"
     gene            5152..5874
                     /locus_tag="PKJ16_06160"
     CDS             5152..5874
                     /locus_tag="PKJ16_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV46608.1"
                     /translation="MKTFIAIVAMALCASAAFAAPGVIIADGARLRRTPSLKGAELAQ
                     MKRGDLVEVAAESKSEEVIDGMRYPWVSITSNGKTGWVYGAYIVHGGPLYYDAKDGTL
                     AWMDTRYLYSNTYKPQFNVINKKKKVDIRFDLEPQDYTTGYALSTGLKYVAIDAGTDV
                     IGSIVIYSIPARRLVHRASYTRHRFEWVGQRLSYQCITYTGDGCLLWEEEVFDDGAVK
                     KTGKKGKGEYHGSGKGVDPRCR"
     gene            5897..6463
                     /locus_tag="PKJ16_06165"
     CDS             5897..6463
                     /locus_tag="PKJ16_06165"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV46609.1"
                     /translation="MKKKSEIGQNDCIHKAAQDHHHEELMARERIRASRMRTAARSLV
                     IIRISCFAIAVVCLAAGMYHLVDTVVFLSNSADTTGIVSGYRESRARYRDEYEPIVEY
                     RIVSDNLYSSNDSRGSEKRVLERIGYSPKKPYEVGSRLDVIYDRHNPGNARLKDFWNL
                     WRAVIGGFASGAFLLALGACLKRIVSAS"
     gene            complement(6654..7187)
                     /locus_tag="PKJ16_06170"
                     /pseudo
     CDS             complement(6654..7187)
                     /locus_tag="PKJ16_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7526..8824)
                     /locus_tag="PKJ16_06175"
     CDS             complement(7526..8824)
                     /locus_tag="PKJ16_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46610.1"
                     /translation="MNVISRRQFLARTAAAAAALYGAERIIDVGAGAFAEERPLYSVK
                     TDLAFVNAAALTALFAEIDRFGTDTDHVRAASALFADGMTLDSFATRYCAAARRANPA
                     KPERNGSYRGVAFARHAEAHLALINRLITMLGNAMFEATARAAWNNLSTADERRIGGD
                     IGDILGALRLVAKRMSLPGLDAAALAPNDMDRDRRVTVHATRRYARTYHNIVVPLYHL
                     ERCRTDFRSPAFGDHAIGLIDYCAYAVRAARYRWKAIFARYALLESFERDYAAVPNGR
                     EAISPSGVIASGKHRLAGKATLAHLYAELSLLRMRVLARQSQSGIFTNEDRQHFMAEC
                     SCLVDATADIAARPTFAKRPIFGGSFAVSPSPKAGAKKTARIAIPALTIAALGLADTS
                     GKRSFAISTPQGAGHAVKRIDDALAIVRRDRAKLREALGK"
     gene            9076..10809
                     /locus_tag="PKJ16_06180"
     CDS             9076..10809
                     /locus_tag="PKJ16_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HNV46611.1"
                     /translation="MKCTCPECARSFSFDETKYNKPTVAFKCPGCGGRIVVELAPPGP
                     LQTAAAVDDADGRKGSLPAEDVQALKNLHRDAESAAIADAIKAVLFLHDGMRPEQAAR
                     LLMMKPDDVEAAWRTYRAKGIDCFAPRFSAASEVSADDKRELLGRAAVDAVRLEIGYE
                     LVPMLDQAQGGTLLERIGRIRTDIASTYGWTLGPVRVIDNMRLGAREYRILVNGVRAA
                     SGEVQPGKVIALQTDAVKKPIDAPHAPEPVFGLPSLWLPADKRAAAEGRGYTVSDPDT
                     VIATHLTEVVVGRAGEIVGLREVNALLASLRERNPDAVAEAESAPGFSPLALLGVVRD
                     LLSERVGVGNLDVILEALAASLPETTEARELTRRVRIALGRQIVEPLLADGVLRVCIL
                     PNEIEDEIGKVVAEIRDERELTAHTRDTLLNSLQGMEWAHPVVMTFGDRRRLLRLLLG
                     VAYPHRIFTVIAEEEVPYGIKVRQDTPFVFEDVLLLDDASIKKIIDLIDRNELAMALK
                     GCETEVMEKFFRNFDEDDCAEFKEEMDFMGPVRLREVEAAQRAIVKAVLQLKASGDIV
                     EYGSGDDELVV"
     gene            11002..12222
                     /locus_tag="PKJ16_06185"
     CDS             11002..12222
                     /locus_tag="PKJ16_06185"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46612.1"
                     /translation="MGKGRPRVLVLTGARQTGKTTLVKRAFPDMPYLSMDDPVVRPTF
                     SRMSAADWVERYPRAIIDEAQKVPSLIETIKAAHDLDPRVLYLLLGSSQILLLSKVKE
                     SLAGRVGILELWPLTLPELATDSWESELRESRLIAWLRDMKAEQAIFSGIPASDRSYA
                     RHLPLFESYVTWGGMPALTMAGMDDDDKEAWLRDYRRTYLERDVADLASLRDLEPFVA
                     AQKAIAVRSAKQVNFSDLARVAHIAPATARRFMRYLELSYQVLVLPPYSRNRDKRIAK
                     MPVVHSLDPGIHRAVVNRRGDPTGEEFESAVVAEIYKQVRSANIAADFHHLRTYDGRE
                     VDLLIELADGFVAIEIKMGDRVSAVDGRHLRDLHGMLDKPLLGSLVLSMDPAINRLAK
                     GIHAVPIAYALSAG"
     gene            complement(12229..16836)
                     /locus_tag="PKJ16_06190"
     CDS             complement(12229..16836)
                     /locus_tag="PKJ16_06190"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46613.1"
                     /translation="MEGILAKLNIYLIPPMLFLATGFSLAIFSLAKGKRQRDTVTFAL
                     LSLWWSLLSIVFVSHHLFKGDTGLIMTIERVVHCAYVFIPFIGIQFFHAMTGYRNRAI
                     VITSFALSAMISLTTFSDHYIYGLWEYQWGYIAKGGIIFQLFGAYSFIVLIYGIVLFI
                     KTMRAEADPVVRLKLRYLMISMFIIALLSQFNIPAMNGIDVYPMGNFAFLPMLLMAWG
                     IYRHDVIQINRYAKRRLTGIAIRIIVVVGLVVMLAVAWWALSGTDFATLARKTVPYGL
                     PPLLSFLFCSFLSVMGLRVGANRRESFVFSFLTLGYTFLSLDIFINCVTDVPEIALRA
                     SRMSHVMVVLLPSLFVHLNRVITRRKSERPLPYVLYAVSALLVPFTQSDWYLSGVYSF
                     SWGFFAKTGLLFNVVILLSTFSTIYSMGIIILAYRNTASAHSKQRLLFFLLGAMSGAI
                     MSMGNFPAMNGMDIYPAGNFIFVPMTFFAIGLYRYNRDEMVLFASNAAYYLVLAACVA
                     ALSAAIARFNDSPLLHAAAVLALVAMIIFAGSWRRLSAKIFSGRKTGLNRTFERLGTV
                     LSKCRSFDEIGRAVAAAAFEDLRAQRCTMLFFDGDSNVFTGLERAHGAPVSVDEAGTA
                     NAEAPLVIDADHPLLALYAQRRPVFRQEEIEEWLLYHDGPLATDDPIRRAELSVPVHY
                     EDHITGIVLMGPKTDGSMYSTEEADFLYRLGLVLGAYIENARILQQLEHTIEERTEEL
                     RASESDFRIIVENASDMIYRMDRVGTCTFANPVILTMLGITKEELYGRFFWEFVPKGS
                     RRPVIAFYARQAADSIPETQYELPIITASGDVLWVNQIVKIVETRNGPEYQCIARDIT
                     ARKKADDARRELEEQKSRFFANISHEIRTPLTLMLSPIESVLQGHYGKSVNREFFNNL
                     HRNGMRLLRLINNLLDFSKIEAGRMTMRVAPVDIVAFARYFAGSVQGAADSRGIALDL
                     DAPQEPLVLHLDTNKLDKVFMNLYSNALKFTGRGGRIGIRIFDDTGECRIEFEDTGPG
                     IPPDKIGIVFDRFSQADTSATRRFEGTGIGLALAREFVEMHGGAIAATSRFIDDHPTD
                     HGTVFTVTLPKGTAHLEGRDGVEFAESDGEERIEDAVIGRGLIAGPESSDPGEPAVTP
                     SIASTDRAPTLAGDAMKTVLVIDDNPDMREYLSQLLNKTYHVHTANDGGAGLDQARAI
                     HPDLIVTDVMMPVLNGFELTDRIRSDDELRSTPIIMLTAGAELVSKITGLERGADDYL
                     VKPFNPIELTARIASLLKGYEYQRILAKRNREIESDLEVARLIMERLLPGDVPTVPGY
                     RSHITYLPMDKVGGDFYDVRFRPEDGTLEVFIADVSGHGLPGAFIALITKMALESIDG
                     GTSVRDVLGAIDEVVRRFMVKSHYVTTFLGHIDTTRNTMRYCRAGHFPPILYRPHEDT
                     FILLQAKGTPLGLQIGIEHEEREIELRPGDRIVLYTDGITECMNSERELFGEERLMEC
                     IREHHALPPAAFSERVLDLLRAFSSSEAFQDDLTMIVLDVE"
     gene            17090..>17377
                     /locus_tag="PKJ16_06195"
     CDS             17090..>17377
                     /locus_tag="PKJ16_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46614.1"
                     /translation="MAHTILLVEDEAILALAESDTLRRYGYAVVTAHSGAAAVSAAKD
                     PAVDLVLMDIDLGPGMDGTEAAARILRDRDIPIVFLSSHTEPAIVEKTETIT"
BASE COUNT         3389 a         4981 c         5723 g         3284 t
ORIGIN      
        1 ccgcgggagc tggtagccga agaagaactt cttgtacagc tgcggggccg cgaggaacgt
       61 ctccgggacg ggcgtctggc cgccgggaag cgactttcgc gtaacggtga tgcggtagcg
      121 cacgttcttc tcccggtcgc gctcgttgta cttgtcgatc cagaggacgt gcggctgccg
      181 gttgccgatg gtcccgaagg ggtagacgaa cgggtcgttg agcccgagcg ggagcgcgac
      241 cgactgcgcg agcagcagga aggcgatgat gagcgcgatc gtccgcggca tgtcagcgcg
      301 ccccgtacgt ctcgtcgatc ctgcccatgg cgcggtcgcg acggaaatcg atgatggagg
      361 tcgcggtctt gacgacgaac atgctggtga agacgaacac gaagagcgtc agggccaaga
      421 ggagcgcgtc ggtcgaaccg agaacgcgct cggcccgttc ggcccgctcc gatccgatga
      481 cggggcgcag cagatcggtg tactcggagg cgccggtcac gaacgtgaag gcgaagccga
      541 tcgcaatacc gaggacgatc tgcgccttgg agagggccat gcggaccttc acgatcttcg
      601 cgtcggtcgc gggggagaag cgatccgcgg catcgcgtgt ccggaaccgc ttcgcgaggg
      661 gcgccatcca gcgtgggtac tcgttcgcag tgatgatgcc gcgcgccatg atgtacatgt
      721 gtgcaagaaa gaagaatgcg cacccggcga tgaaatacgc gagcgccgcg aagatgggcg
      781 cgcggtgact gatgaaaagc ccgatggcgc cggccatcat catccccaca acgatgagaa
      841 cgaacatcga tacgccgatc gcgaagatga gcacgaagcc cgacacggac ggcgcgagcc
      901 ggtcgcggat gtcgtgctcg cgcggggaga ggagcagccc gaaaatgagg acgaagaagg
      961 aacacaatgt tccgatgaag cacgcgccgc ccgcgtacat gagcgcggac cccgccatcg
     1021 acaatgccgg gtgcgtgaag atgcccagga tcgagcccag aggcaggagg accatggccc
     1081 ccatgaggcc gcagatgaag ccgtagagcc ccgcgcgcgc gatgcggacc ttgcgctcga
     1141 cggcggcgtc gatgttgaat ggcgtgctca tgtttgtccc ggtgacgatg cgcaccatac
     1201 cgatgcgggc ggcgttgtca atcacgaata atcgacagcg agcgcgcggt attaacgaac
     1261 aaaatcatgt tgacttttcc ggggaatgcg gtagtggtga gagtgggacg ccgggacata
     1321 aaaaaacatt gaggtttccc catgaccgac ataatgcccc aggacgaaat cgacgcattg
     1381 ctggctgagg tgtccggtga gacggtgaat tcaacagcct gccgcatcga cgggaacgat
     1441 aatccagata tgcgcccgaa gctgtacgac tttcgcaggc cgcagttcat gacaaaatcc
     1501 gccgagtccc ggctgcgcga tatcttcgcg accgcctgcg atgggatcga ggccgcgctc
     1561 ggagaaagcg ccgggatcgc gtgcgaccgc ggatcgatca ccgtcgacca gctcacctgc
     1621 ggggagtatg tccggtcgct catgagcccc acgctcttct cgacgataag aattgaaccg
     1681 ctgccgggat atgtcgccat tgaggtgatg ccgtcactcg cgtgcgcgct catcgacgca
     1741 atggcgggtc gcgacatccg tgacgtgtct ctcaatcttg gttttactgc ggacgaggcg
     1801 cgcgaggccg cagcgcctct tggcgatatc gccgaatcca tatgccgcgc cttcggcgag
     1861 agtgccgaac tccgctcccg agtgaaggcg atctattcca atccccgtga cgctgagttt
     1921 ttcggagatg acgagatggg tgtctgcgtt acgtgtgcgg cgaaaatcga ttacgttgag
     1981 ggactcatca gcgtcttttt gccgcgaatc tccttcgggc cggcgctgtc cctgctggag
     2041 gaaagcgctc cggccgccga tgttctcggc aacagagtca tcgatgtccc gctcaaaacg
     2101 cgcgtcgcca cgctcgacca ggtgagaaaa ctggacgccg gtcgcaagct tgagatagat
     2161 accgcggcaa atccgacagc tgcgatgcgc gcgccgatgg aggaataggt ctcatgaatt
     2221 ccgatttcct gaaaaaactg aaacttccac tgcgcctcag gctcccgctc gacctgacca
     2281 tcggtgatac gggttctctc gttgtccgtc gcgatctctc cgaatatcag gggaacgagg
     2341 gggcgctgta tcttggcggt atgaacgtcg cggaagccgt gctcaggcat catgcgggca
     2401 aggatgtgta ttatatcgag attgttgccc ctcgagagaa tgtcgatgat gaccggctgc
     2461 tcgcctcgac ggttcgtttg gagctggtct tcggtattgc cgaacagccg gagaacatgc
     2521 cgctcgagct tgcgcatgga gcgacagttc ctttgcgccc cctgtatccc ataaaagaaa
     2581 taatgaagaa tgatgcggcg ggcgttgcgg agcatgaatc ctcatcggaa ccggcaccgg
     2641 atccgtccga gccaacagaa tcatcggcaa aaaaaggccc catgtccatg gaggagatcg
     2701 acgatctcct cgccgcgatt tcaaaaggcg atgcgggggc ctccccgtcg tccgcacatg
     2761 ccggtgtcgc gctttcgctt gacggtctca ttgtcgcgaa gggcgagacc gtgctcgttg
     2821 acgaccgttt cggcatccgc attaccgaag cggtggaggg ggacgatcgc ttcgcgctga
     2881 tggagcggcc cgacgcgggg ctcattcggg gcgttgcggt gcttgcatcg cgatccatcg
     2941 cactcgccga tcttcccgat ctcaccgagg gttccgtcat caccatggat gcgaccgcac
     3001 tcggtccgtc ggtgctgcgg ttcgataacg gtatcgttca tgcgggtgag atccttttcg
     3061 gcgatgccca tgatgccgga tgggccttcc gcaggcttta ctcgttggag gaaataacgc
     3121 ggagtctgat gcaggacaga atgcgggctg cggcgttcgc ttccgatgac ggggagggcg
     3181 atattgcgga tggtgccacc gatgtcgatg aggtggcggt gtttatcgat tcgcctatgg
     3241 atgccgcagg tgccactatg ggcaacgagg atatgagcgc tgaggacgcg aacgcccggc
     3301 gttgggcggc gctggaggcg gcggaagcgg aaatgacgga cgagcacgaa tcgactcccg
     3361 aggaactgtt ccctgatgaa attcggcagc agttgctcga gcatgcccag tcgatcgatt
     3421 tcatggacgc gccctccttc acgaaggaaa acctgctaac gttgtcccgg atcattaaga
     3481 acatgtgcct ctcgatgggc ggcgccattg acgatcttct ctcgcttgag cgctccgtgc
     3541 acctgcgcct gaagcgcgcc cgcgtggcgc cgttctccga attccatgtc gacgacgggt
     3601 tggacagcat gccgtggagc atcgagctcg atcccaatct gggcttcgtc ttcgtcaagc
     3661 cgggtcgcgg ccccctggtg tccccggagg gtgtcgacaa agttctggag ctaatgggag
     3721 agacaagcgc tgattcatcc tcgaacatat ccatagagac gtgggtgcac atgtacctga
     3781 ccctcacgat ctgcgatcag ttgataaggg ccttcgagcc gctggtcgcc atccagaccc
     3841 gcacgcagtc atttgatgag aacgcggaaa cgggcgccgg gttcgtctcg cccgtaacgg
     3901 cggtgcccgc cgacgcgcgc tcggcgctct tcgagatcgc catcgaaaac ggcgacgtgc
     3961 gaaacgcgat cacgctggcg atgccgctcg cgggcctcgc gccgatcgtc ccgatactgg
     4021 agaaagagta tctggacggc acgatggagg ttcccgggga gatccggagc ctcttcgcgg
     4081 gacgggatga tgatgtcgcc gatgaacaag acccgagcga gtgcgacacc tatcctgagg
     4141 acatgccgtg ggagcggcgc cttcccatcg cctgttatct catggatcat gccgccgtac
     4201 gcgaattcct cgaccagggc ggcgatccga atatccaaga cgaggacggc gagacgcccc
     4261 tcgaaacgct cgccgcgaac tggttcgacg ccgatccggg cgtggagctg acccgggtga
     4321 aggagatgca cgggatccgc atggagcgca tcgtaggacc cgacgatgcg cgggagtgcg
     4381 cctatcttct cctcgacgcc ggcgcgcgcg tgacgaagct cgcaggggag cgcgcgaagg
     4441 aaggctcgtc gatcgatggg gcgcccaagc gcgagcttat tcgattgctt cgagagtgcc
     4501 tgggcgcacg taaagacggc acgccgttcg agcgctggtg ggagattcct gcacgcaccg
     4561 attcaaggga agcaagcgaa acgaaggtta tgtcggagga ccagatcgac gatctcctcg
     4621 catcgatctc ctccgaatcg accggcccgt ttgcgcatgc cgaggagctc tttgccgagg
     4681 gggagtacct ggaggcaatt gacgcctatt tgcaggcgat gaaggaggcc ggggagaatg
     4741 cggcggccca caacaatatc ggcctgtcct acctgaatct tggggaatac gaaaaggcac
     4801 tgaagtactt caagaagtcg ctccaggtcg atccggatta cgcgaccgcg cttgcaaacc
     4861 aggcctgcgc gtacaagcgt atgggcgata tcgaccgtgc gatcgcggtt tccacggagg
     4921 cgctcgcccg cggggaaacc ggcgatatcc tgtacaatct cgcgtgctat accgccatca
     4981 acggggacgc gaaaactgcg atggcgtatt tgaagcgcgc cattgatcac gatgaacgct
     5041 ttcgcgagat ggcggccgat gacgatgatt tcgccgctct cgaggactca aaggattttc
     5101 ggaagctggt gggatgatcc aaccatgagt gcggtgcgcc gggaggatgt gatgaaaacg
     5161 tttatcgcaa tcgtggcaat ggcgttgtgt gcgtcggcgg cattcgccgc gcccggcgtc
     5221 atcatcgccg acggggcgcg cttgcggaga acgccgtcgc tcaagggcgc cgaactcgcg
     5281 caaatgaagc gcggcgatct cgtcgaggtg gccgccgaat cgaagtccga ggaggtgatc
     5341 gacggtatgc gctatccgtg ggtttcgatc acgagcaatg gcaagacggg ctgggtgtat
     5401 ggcgcgtaca ttgtccatgg cgggccgctg tattacgatg cgaaggacgg gacgcttgcg
     5461 tggatggata cgcgctatct gtattccaac acatataaac cgcaattcaa cgttatcaac
     5521 aaaaagaaga aggtcgatat ccgcttcgac ctggagccgc aggactacac cacgggttat
     5581 gcgctctcga cgggactcaa gtatgtcgcg atcgacgccg gcactgacgt tatcgggagt
     5641 atcgttatat attcaatacc cgcacgtcgc ttggtacacc gggcttccta tacacgccat
     5701 cgcttcgagt gggtggggca acgcctttcc taccaatgca tcacctatac cggggacggc
     5761 tgtttgctct gggaggagga ggtcttcgac gacggcgcgg tgaaaaaaac cgggaagaag
     5821 gggaagggcg agtatcacgg atcggggaaa ggcgtggatc cgaggtgtcg ttagactaaa
     5881 tcgggagttg ttgttaatga agaagaagtc tgagatcgga caaaatgatt gtatccacaa
     5941 ggctgcccag gaccaccatc atgaggagct tatggcgcga gaacggatcc gcgcttcacg
     6001 aatgcgaaca gcagctcgat cactggtaat tattcgaata tcgtgctttg ccattgctgt
     6061 tgtttgcctg gccgcaggca tgtatcatct tgtcgatacc gtcgtctttt tgagtaattc
     6121 ggcagatacg acaggaatcg tttctgggta ccgggagagc cgggcgcgtt accgtgacga
     6181 atacgagcca attgtcgagt atcgaatcgt atcagataac ctgtattcgt cgaatgattc
     6241 acggggttct gaaaaacggg tccttgaaag aatcgggtat agtccgaaaa agccgtacga
     6301 agtcggcagt cggctcgatg tcatatatga tcggcataat ccaggaaacg cgcgcctgaa
     6361 ggatttttgg aatctatgga gggctgtgat cgggggtttt gcgagcggcg cgtttctact
     6421 ggcgctcggt gcatgcctga aacgaattgt atcggcatca taacagtcgg tgtcattcaa
     6481 taattctcta tcacgatcgt tcgtttcaac tctccggcgg cctcgagctc gcgcgccttt
     6541 cccgcaagat aatcgcgctc ctttgttgcc ttcagtactg ccgcatcgat aattgcagcg
     6601 gcattgcccg atggcgccgt gagatcgatc gcggttcgcc attgtttgtc gcgcagcccc
     6661 agcgagcggg catggatggt gttgatgtac acacgctccc ggcgcttttc gtcgccgccc
     6721 gtaacgaacc acagcgaggc ggtccacccg cgcgcccctg gtcccgcaaa agtccccgtt
     6781 agcaatttca tacggttgta ctccgtctgc gaggcaacgc ggtcgacctc gtcgatgagc
     6841 tgcgacacct cgacctgaag gtggagtcgc atctccggtt ttttcgcgac ggccagccgc
     6901 gcgagttcgc gcatccgctc aagtattttc agatcttcag ccagaccgga catcgcggtt
     6961 cggacgagct cggactggcg tgtgcgaaca tcgtattcaa gtgaaagctt ctcgaaggcg
     7021 gcggccggct gattggcaag cgcgcccgtg gcgagcgaca ggcggttttc gtaggcctgg
     7081 atgtcggcgc ggcacttcgc gatcttggcg cgggccccgg cgagctcctc gacgcgcgtg
     7141 ggctggccct ccgagccgcc gcggaggttg agtccctgcg atgtcatcgt cgcgatgaag
     7201 atgcgttttc gcgaatgtat ccggggcgtg acgcagagcc acatcgatgc gcgcgggttc
     7261 catttcgcat agcgtccgtc caggatcggg gatccgttgt gggcgcagcg caccgccgtg
     7321 cggtccattt cgtcaaggat cgcgtcgatt atcttccggg tggatgaccg tttctgcagt
     7381 gcagtttcta tggccgcaag atgttcctcc tgccgggcca gcgcggccct gatgcgcgcg
     7441 aagagctgcg acactgcgcg gaactcattc gcgacggtct cccggcgcgg cgcggccgtt
     7501 acggcgaagg gattgacgcg gatcttcatt tgccgagggc ctcccggagt ttcgcgcgat
     7561 cgcggcgcac gatggcgagc gcgtcgtcga tccgtttcac ggcgtgtccc gctccctgcg
     7621 gcgtgctgat ggcgaaggac cgcttgccgg acgtgtcggc gaggcccagc gcggcgatgg
     7681 taagggccgg gatggcgatg cgcgccgttt tcttcgcacc cgctttcggc gacggcgaaa
     7741 cggcgaacga cccgccgaag atgggccgtt tcgcgaaggt cggacgcgcg gcgatgtcgg
     7801 cggtcgcgtc caccaggcag gagcattcgg ccataaaatg ctggcggtcc tcattggtga
     7861 aaatcccgga ctgcgattgc cgcgcaagga cgcgcatccg gaggagcgag agttcggcgt
     7921 acagatgcgc aagggtggcc ttgcccgcga ggcggtgttt cccggacgcg atcacgccgg
     7981 atggcgaaat cgcttcccgc ccattcggga cggcggcata atcccgctcg aatgactcga
     8041 ggagcgcgta tcgagcgaag atggccttcc atcgataccg agcggcgcga acggcatacg
     8101 cgcaataatc gataagaccg atggcgtggt cgccgaaggc gggcgatcgg aagtccgtcc
     8161 ggcagcgctc aagatggtat agcggtacaa cgatgttgtg ataggttcgc gcgtaccgac
     8221 gtgtcgcatg gaccgtgacg cggcggtccc ggtccatgtc gtttggcgca agcgccgcgg
     8281 cgtcgaggcc gggaagcgac atgcgtttcg cgacaaggcg cagtgccccg agaatgtcgc
     8341 cgatatcacc cccgatccgc cgctcgtccg cggtggagag attgttccag gcggccctgg
     8401 ccgtcgcctc gaacatggcg tttccgagca tggtgatgag gcggttgatg agcgccaggt
     8461 gcgcctccgc gtggcgggca aaagcgacgc cgcgatatga tccgtttcgt tccggtttgg
     8521 ccggattcgc gcggcgcgcg gcggcacagt agcgggttgc gaacgaatcc agcgtcatcc
     8581 cgtcggcgaa gagcgcggag gcggcgcgaa cgtggtcggt atcggtcccg aagcggtcga
     8641 tctccgcgaa gagcgcggta agcgcggccg cgttcacgaa ggcgagatcg gtcttcacgc
     8701 tgtacaacgg gcgctcctcg gcgaaggccc ccgcgccgac gtcgatgatc cgctccgcgc
     8761 cgtagagcgc cgccgcggca gcggcggttc gggcaagaaa ttgtctgcgg gatatgacgt
     8821 tcatggtgct gtggtattgt tcgaatgcat acgtatctaa tacgcatggt acgcgcgtgt
     8881 caactcgatt attgcttgcg gaattgctgc gagacggata ccatcatcca cggaacagat
     8941 ggtgtgccca tgctattgtg tcgcgtgtgc ggtgtggacg gcgcgtatgt gaatgtgaac
     9001 actcgctatg ccctggcgtt ggtcccgagt cgggcgtccg gttccggagc ggatactact
     9061 acgagaggtg gcgtcatgaa atgcacctgt cccgaatgcg cacggtcctt cagcttcgat
     9121 gaaacgaaat acaataagcc gacggtcgcc ttcaagtgcc ccggctgcgg cgggcgcatc
     9181 gtcgtcgagc tcgcaccgcc cggtccgctt caaacagcgg cggcggttga tgatgccgac
     9241 ggccgaaagg gatcgctgcc cgctgaggac gtacaggcgc tgaagaacct gcaccgcgat
     9301 gcggaatcgg ccgcgatcgc cgatgccatc aaggcggtgc tgttccttca cgacggcatg
     9361 agaccggagc aggccgcgcg cctgctcatg atgaagccgg acgatgtgga ggcggcatgg
     9421 cgcacttatc gcgcgaaagg aatcgattgc ttcgccccgc gcttctccgc cgcatcggag
     9481 gtttcggccg acgataagcg cgaactcctc ggcagggccg cggtcgacgc cgttcgcctg
     9541 gagatcgggt acgagctggt cccgatgctc gatcaagcgc agggtgggac gcttctcgag
     9601 agaatcggcc gcatcagaac ggatatcgca tcgacctacg gctggacgct ggggccggtg
     9661 cgggtgatcg acaacatgcg gctgggcgcg cgcgagtacc gcatcctggt gaacggcgta
     9721 cgcgccgcct cgggcgaggt gcagccgggc aaggtgatcg cgctgcaaac cgatgctgtg
     9781 aaaaagccga tagacgcgcc gcacgcgccg gagccggtct tcgggctgcc gtcgctatgg
     9841 ctacccgcgg acaagcgcgc cgcggcggag ggtcgcggct acacggtttc cgatccggac
     9901 acggtgatcg ccacgcatct cacggaggtc gtcgtcggcc gggcgggcga gatcgtggga
     9961 ttgcgcgagg tgaacgcgct gctcgcgtcc ctccgcgagc gcaatccgga cgcggtcgcg
    10021 gaggcggagt cggcgccggg gttctcgccg ctcgcgctgc tcggcgttgt gcgcgatttg
    10081 ctgagcgagc gcgtgggcgt gggcaatctg gacgtcatcc ttgaagcgct ggcggcgagc
    10141 cttccggaga cgacggaggc acgggagctc acgcgccggg tgcgcatcgc cctcggacgg
    10201 cagatcgtcg agccgctcct tgcggacggc gttttgcgtg tgtgcatcct gccgaacgag
    10261 atcgaggatg agatcgggaa ggtcgttgct gagattcgcg acgagcggga gctgaccgcg
    10321 cacacgcgcg atacgctgct gaattcgctt cagggcatgg aatgggcgca tcccgtggtg
    10381 atgaccttcg gcgaccgccg ccgcctgctg cgcctgctgc tgggcgtcgc gtacccgcac
    10441 aggatcttca ccgtgattgc ggaggaggag gtgccgtacg gaataaaggt gcggcaagat
    10501 acgccgttcg tgttcgagga tgtccttctg ctcgatgacg cttcgatcaa aaagatcatc
    10561 gacctgatcg accgcaacga gcttgcgatg gcgctcaagg gctgcgaaac ggaggtcatg
    10621 gagaaattct ttcgcaattt cgacgaggat gactgcgcgg agttcaagga ggagatggat
    10681 ttcatggggc cggtgcggct tcgcgaggtc gaggcggcgc agcgcgcgat cgtcaaagcg
    10741 gtgctgcagt tgaaagcgag cggtgatatc gttgaatacg gctcgggcga cgatgagctc
    10801 gtggtgtgag gagaggtgag gtggggtgtg gattgtgatt ctcgggaatg ggtcttcttc
    10861 gtaatcaatc cataaggtat agaattgcga aaaatagttg acgatagttt aatattggcg
    10921 taatattgat aataatatat caaaattacg ccaatattga gtatgaaaag tagattcctc
    10981 gcaaaaaaca tcatcgagag aatgggtaaa ggaaggccac gagtgttggt gcttaccggt
    11041 gcgcgtcaaa ccggcaagac gacccttgtg aaaagggcgt ttccagatat gccgtatctc
    11101 agcatggatg atcccgtcgt ccggcccaca ttctcgcgca tgtcggcggc ggactgggtc
    11161 gagcgctacc cgcgcgccat catcgacgag gcccaaaagg ttccgtcgct cattgagacc
    11221 ataaaggcgg cccatgatct ggatccgcgg gtgctgtacc tgcttctcgg gagcagccag
    11281 atactcctgc tttcgaaggt caaggaaagc cttgcggggc gggtcggcat actggaattg
    11341 tggcccctga cccttcccga gctggccacg gactcctggg aaagcgaact gcgggaatct
    11401 cgcctcatcg cctggctcag ggacatgaaa gccgagcagg cgattttttc cggcattccg
    11461 gcgtccgacc gctcctatgc ccgtcacctg ccgctcttcg aatcgtatgt aacctggggc
    11521 ggcatgccgg ctctcacaat ggcgggcatg gacgatgacg acaaagaagc gtggttgcgt
    11581 gattatcgac ggacctatct tgagcgcgac gttgccgatc tcgcgagcct gcgcgacctg
    11641 gaaccgttcg tcgcggcgca aaaggcgatc gccgtacgga gcgcgaagca ggtgaatttc
    11701 tccgatcttg cgcgcgtcgc gcatatcgcg cccgcgacgg cccggcggtt tatgcgctat
    11761 ctcgagctga gctatcaggt cctggttctt ccgccctatt cccgcaaccg cgacaagcgt
    11821 atcgcgaaga tgcccgtggt ccattctctt gatccgggaa ttcatcgggc ggtggtcaat
    11881 cgtcgcggtg atccgaccgg cgaggagttc gagtccgccg ttgtcgcaga gatctacaag
    11941 caggtccgga gcgcgaacat tgcggcggat tttcatcacc tgcgcaccta cgacgggagg
    12001 gaagtcgatc tgctcatcga acttgcggac ggctttgtcg cgatcgagat caaaatgggt
    12061 gatcgagtgt cggctgtcga tggccgccac ctcagagacc tgcacggaat gctcgacaag
    12121 cccctgctcg ggtcgctggt gctgtctatg gatcctgcta tcaaccgtct cgcgaagggc
    12181 atccacgccg ttccgattgc gtatgcgctc tcggccggct gacctccctc actccacgtc
    12241 gaggacgatc atcgtgaggt cgtcctggaa cgcctcggac gacgagaacg cgcgcagaag
    12301 gtcgagcacg cgctcggaga aggcggccgg cgggagcgca tggtgctcgc gtatgcactc
    12361 catcaggcgc tcctcgccga agagctcgcg ctcagaattc atgcattccg tgattccgtc
    12421 cgtatagagc acgatgcggt cgccggggcg aagttcgatc tcccgctcct cgtgctcgat
    12481 gccgatctgc aaacccagcg gggtcccctt cgcctgcagg agaatgaacg tgtcctcgtg
    12541 cgggcgatag agaatcggcg ggaagtggcc ggcccggcag tagcgcatgg tgtttcgcgt
    12601 cgtgtcgatg tgtcccagga aggtggtcac atagtggctc ttgaccatga accggcgcac
    12661 tacttcgtcg atggcgccca gcacgtcgcg caccgacgtg ccgccgtcga tgctctccag
    12721 cgccattttc gtgatgagcg cgatgaacgc gcccgggagg ccgtgtcccg acacgtccgc
    12781 gatgaacacc tccagggtcc cgtcctccgg acgaaaccgt acatcgtaga agtcgccgcc
    12841 gaccttgtcc atcggcaggt aggtgatgtg cgaacggtac cccgggaccg tggggacatc
    12901 gccgggcagc aggcgctcca tgatgagccg cgcgacctcc aggtcgctct cgatctcgcg
    12961 gtttcgcttc gcgaggatcc gctgatattc gtagcccttg aggagcgagg cgatgcgcgc
    13021 cgtgagctcg atggggttga acggcttcac gagataatcg tccgcgccgc gctccaggcc
    13081 ggtgatcttg ctcaccagct cggcgcccgc ggtgagcatg atgatgggcg tgctcctgag
    13141 ctcgtcgtcc gagcggatgc ggtcggtgag ctcgaagccg ttcaagaccg gcatcatcac
    13201 atcggtcacg atgagatcgg ggtgaatcgc gcgggcctga tcgagcccag cgccgccgtc
    13261 gttggcggta tggacatggt aggtcttgtt taacagctgc gagaggtact ctcgcatgtc
    13321 cgggttgtcg tcgatcacga ggaccgtttt catcgcatcg ccggccagcg tcggcgcgcg
    13381 gtcggtgctc gcaatcgacg gcgtgaccgc gggttcgccc gggtctgagg attccggccc
    13441 cgcgatgagg ccgcgaccga tcaccgcgtc ctcgatgcgt tcctcgccgt ccgactccgc
    13501 gaattcgacg ccgtcgcggc cttccaggtg cgcggtcccc ttcggcaggg taacggtgaa
    13561 aacggtcccg tgatcggtag ggtgatcgtc gataaatcgg ctggtcgcgg cgatcgcgcc
    13621 gccgtgcatc tccacgaact cgcgcgcgag cgccaggccg atgccggtcc cttcgaagcg
    13681 gcgggtggcg ctagtatcgg cctggctgaa gcggtcgaac acgatgccga tcttgtcggg
    13741 cgggatcccg ggcccggtgt cctcgaactc gatgcggcac tcgcccgtat cgtcgaagat
    13801 gcgtatgccg atgcggccgc cgcgcccggt gaacttgagg gcgttggagt acaggttcat
    13861 gaagaccttg tccagcttgt tcgtgtccag gtgcagcacg agcggttcct gcggcgcatc
    13921 gagatcgagc gcgatcccgc gcgaatccgc ggcgccctgg acggaccccg cgaagtagcg
    13981 ggcgaaggcg acgatgtcga ccggggcgac gcgcatcgtc attcgccccg cctcgatctt
    14041 cgagaagtcc agcaggttgt tgatgagccg caacaggcgc atgccgttgc ggtgcaggtt
    14101 gttgaaaaac tcacgattca cgctctttcc gtagtgtccc tgcaggaccg actcgatggg
    14161 cgagagcatg agcgtgagcg gcgtgcggat ctcgtgcgag atgttggcga agaagcgcga
    14221 cttctgctcc tcgagctcgc ggcgagcatc gtcggccttc ttgcgcgcgg tgatgtcgcg
    14281 cgcgatgcac tgatactcgg gaccgtttcg cgtctcgacg atcttcacga tctggttgac
    14341 ccagagcacg tcgccggagg cggtgatgat gggaagctcg tactgcgttt ccgggatgct
    14401 gtcggccgcc tggcgcgcgt aaaacgcgat caccggcctg cgtgagccct tcgggacgaa
    14461 ttcccagaag aagcgaccgt agagctcttc cttggtgatg cccagcatgg tcaggatcac
    14521 cgggttggcg aaggtgcacg tgccgacccg gtccatccgg tagatcatgt cgctcgcgtt
    14581 ttccacgatg atgcggaagt cgctctcgga cgcacggagt tcctcggtgc gctcctcgat
    14641 cgtatgctcg agctgctgga ggatgcgggc gttctcgatg taggctccca ggacgaggcc
    14701 cagccggtag aggaagtcgg cctcctcggt cgagtacatc gatccgtcgg tctttggccc
    14761 catcaggaca atgccggtga tgtggtcttc gtaatggacc ggcaccgata actcggcgcg
    14821 gcggatcggg tcgtcggtcg cgagcggacc gtcgtggtac aggagccatt cctcgatctc
    14881 ttcctgccga aacacgggac ggcgctgggc gtacaaggcg agaaggggat gatccgcgtc
    14941 gatgacgagg ggcgcttcgg cgttcgcggt cccggcctca tcgacactga ccggcgcgcc
    15001 gtgagcgcgc tcaaggccgg tgaagacgtt cgagtcgccg tcgaagaaga gcatcgtgca
    15061 gcgctgggcg cggagatctt caaacgccgc agcggcgacc gcccggccga tctcgtcgaa
    15121 gctcctgcac ttcgaaagaa cggtgcccag ccgctcgaag gtgcggttga ggccggtctt
    15181 gcgcccgctg aagattttcg cggagaggcg ccgccaggag ccggcgaaga taatcatggc
    15241 gaccagtgcg agtacggccg cggcatgcag caggggcgag tcgttgaacc gcgcgattgc
    15301 cgcggacagc gccgcgacgc aggcggcgag caccaggtag taggccgcat tgctcgcgaa
    15361 caggaccatc tcgtcgcgat tgtaccgata gaggccgatg gcgaagaagg tcatcgggac
    15421 gaagatgaag ttcccggcgg ggtagatgtc catgccgttc atggccggga agttgcccat
    15481 ggacatgatc gccccggaca tggcgcccag caggaaaaac agcaggcgct gcttgctgtg
    15541 cgcgctggcg gtgttccgat acgcgagaat gatgatgccc atcgagtaga tcgtcgaaaa
    15601 cgtcgagagc aggatcacca cgttgaaaag aaggcctgtt ttcgcgaaaa aaccccacga
    15661 gaaactgtac acgccgctga gataccagtc cgattgcgtg aacgggacca ggagcgcact
    15721 cacggcgtag aggacatatg gcagggggcg ctcgcttttg cgtcgggtta tcacgcgatt
    15781 gagatgcacg aacagcgacg ggagaagcac caccatgacg tggctcatgc ggctcgcgcg
    15841 cagggcgatc tcgggaacat cggtcacgca gttgatgaag atgtccaggc tcaggaaggt
    15901 gtagccgagc gtcaggaagc tgaagacgaa cgattcgcgc cggttcgccc cgacgcgcag
    15961 gcccatgacc gaaagaaacg aacagaacag aaacgacagg aggggcggca gtccgtacgg
    16021 gacggttttt cgggcaaggg tggcgaaatc ggtcccggac agcgcccacc aggcgaccgc
    16081 cagcatcacg acgaggccga caaccacgat gatccgaatc gcgatccccg tgagcctgcg
    16141 tttggcgtac cggttgatct ggatcacgtc gtggcggtag atgccccagg ccatgagcag
    16201 catcggcagg aaggcgaagt ttcccatcgg gtagacgtcg atcccattca tggccggaat
    16261 attgaactgc gacaacaatg cgatgatgaa cattgagatc atcaggtagc gcagcttcag
    16321 gcgaacgacc gggtcggcct cggcgcgcat cgttttgatg aacaggacga tgccgtatat
    16381 cagcacgatg aagctgtatg cgccgaacag ctggaagatg atcccgccct tcgcgatgta
    16441 tccccactgg tattcccaga gtccatagat gtaatggtcc gagaacgtgg tcagcgaaat
    16501 catcgcgctg agcgcaaagg acgtgatgac gatcgcgcga ttgcgatacc cggtcatggc
    16561 gtgaaagaac tggatgccga tgaacggaat gaacacgtac gcgcagtgca ccacgcgctc
    16621 gatcgtcatg atgagtcccg tgtctccctt gaacaggtga tgggagacga agacgatgga
    16681 gaggagcgac caccagaggc tgaggagggc gaaggtgacg gtatcgcgct ggcgcttgcc
    16741 cttcgcgagc gagaagatgg cgagtgaaaa acccgtcgcc aaaaagagca tcggcgggat
    16801 gaggtatatg tttagctttg cgaggatgcc ttccatgttc cgttcgctat atatattccg
    16861 caataatcga tttagatctc atgatttctg tcaagacaat ctctggttcg cggtcagctg
    16921 ccatccgctc gtagcgggat caaaaaatgg aaaaaagcgc ttgatttcct tcttcgaggg
    16981 ggggctgata tcgccatggg atcgctcgat cccatggcgc atcgcggccc cgatccgggg
    17041 ggcgaagggg aacacagatg aagaccgcga tgcgtacggg agaagcggaa tggcgcatac
    17101 catcttgctc gtcgaggatg aggccatcct cgcccttgcg gaatcagaca cgcttcgacg
    17161 atatggctat gctgtcgtga cggcgcattc cggggccgcg gcggtgtcgg cggcgaagga
    17221 cccggcggtc gacctggtcc tcatggacat cgacctgggc ccgggcatgg acggcaccga
    17281 agcggcggcc cgcatcctgc gcgatcgcga catccccatc gtcttcctct ccagtcacac
    17341 cgagcccgca atcgttgaga agaccgagac gatcacc
//
LOCUS       DAORHF010000049        17191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2432_length_17191_cov_6.008462, whole genome shotgun sequence.
ACCESSION   DAORHF010000049 DAORHF010000000
VERSION     DAORHF010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17191
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2432_length_17191_cov_6.008462"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PKJ16_06200"
     CDS             complement(<1..749)
                     /locus_tag="PKJ16_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46615.1"
                     /translation="MKIAVRRISRAVCAVMVLSAIIAFQSNTAHARVGGGSSYKSADA
                     ERKSPRAVTPDGPEKAVDGAPARERDTRDRERERSDWLRDQPRPTQADFDSFRWFGIA
                     ATVFLLVVGIGITALGIFKMDRSSGYLKGVLAPVGAINAAVWLLAWAAMRNFSIPFGF
                     LYASMAVTAVALVVDGLRSAHRGAGGADGVAASPLVVEKTNAGENGRFDLQREDPGFS
                     LILLTDFIHALYHELKGRIGTDSYEELRPFIG"
     gene            complement(882..1253)
                     /locus_tag="PKJ16_06205"
     CDS             complement(882..1253)
                     /locus_tag="PKJ16_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46616.1"
                     /translation="MIKVNLALELPHGSTPVMEGHVSIYIEDAPDPKLYYRFDSDICC
                     HATGEERTCYGCRYYSPWFGGCTTAVETSEGKLYQMHPSMLYTLFISMFRENLDLRGE
                     VSSLRHALIHLCSNDVQRTMA"
     gene            complement(1451..1765)
                     /locus_tag="PKJ16_06210"
     CDS             complement(1451..1765)
                     /locus_tag="PKJ16_06210"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46617.1"
                     /translation="MNVTIREQPPHTVIGIVGEITIYNVTKLNDTVGTVADGSVRSLV
                     IDLERVTAIDSNGIAALYAAKRKIESTKGSFYIANPMPSIRNVLKLTGLSFNFIDLSA
                     SA"
     gene            complement(1843..4590)
                     /locus_tag="PKJ16_06215"
     CDS             complement(1843..4590)
                     /locus_tag="PKJ16_06215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV46618.1"
                     /translation="MKIKYKFIGIFLGALIAIATPLSLFIINQQEHEHIALLHHHGTT
                     VSRILAQSALTILLMNAGRIDMARVDATEMIATFAHLRDDGLLYADIVMISSRPEING
                     AVLSKKTRADHAALPYFSRGRISPEETAGLLDGGSVRETQIDGVAGTCYVFASGAGPR
                     NRPPLCVGRLVFSKDEVLRPVARMRRIITTALGAAVFVLVMLGIVLGNVIAAPVMRLT
                     RAAERIERGDYTTPVRAGGSDELATLSAAFNHMSGIITQKIDELERTNRKLTELDRLK
                     DEFLANTTHELRTPLNGIIGIAESLVAGAAGPVSDDLRHDLDLIIASGRRLNRLVGDI
                     LDFSKLRNNDFVILPAPLDLFNAAQFVISILRPLAVRKGLVLVNDVSPSLPPIMADEN
                     RLQQILLNVVGNAVKFTDEGSVTVSAAPDRADPSRIAISVADTGIGIAPEQQSVIFDA
                     FRQADGTTSRRQDGAGLGLAIVKHLVELHGGAIRIESEPGRGATFIVTLPVAAKSVDD
                     ALAPPVAALAEAAPLDADIRRPVPGAEDTGKTIMVIDDDPVNLQVIINHLALEGYRIA
                     SYHDGVEAYQRIEEGETPDLMLVDVMLPVMSGYELCRLVRRSHTMFELPIVMLTAKNR
                     PEDVVTGIEAGANDYLTKPVQRDELIARVKNLIALRDSVHEHGQLERIRYQLEIATAI
                     QKAVLPQRVPECGKIRTAVRYRPAVGVGGDLYDFFPVDNARMGVLIADVSGHGIPAAI
                     IGAKTEMAFSIYRDNIARPAELMATINRVMTDHSYGQFITACYLYIDLEARAIRYSNA
                     GHKQLLVYRARSSRLEALHIKGAPLGLSTEYEYRELSLAVERGDRIILITDGIPDTRN
                     HQRELFGDERLGGLITQHRALPPEGLADAIFAALDDWKGAAHVDEHDDDATLIILDID
                     Y"
     gene            4726..7005
                     /locus_tag="PKJ16_06220"
     CDS             4726..7005
                     /locus_tag="PKJ16_06220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HNV46619.1"
                     /translation="MRRYAPIPVRSLRALAIAFGILASAAIPQRALHAAIAIQGVVVD
                     RATNRPSGGATVGIIETGQTAIADEKGRFRIALPRPGRYTLAAHSPGYEQTRLVITAD
                     PRKTYMVYLTQRVITTREIVVTAKKEKQNVSRRTLEREEIKKVPGGAGDLMRSIQTLP
                     GVASAGDISGQLYVRGSGPYDNRILIDKFWLANAYHYGGFVSVINTDLIDSIDFYSGA
                     FPAIFGEATGSVLELHTREKEEPTWGGKINVNLLTADAVLEMPLSENGYLILSGRRSY
                     FDLYAEKFLSNMDQVRVTEMPVFWDYQAKAGYQFSKGNRIEFLSYGFEDQVGVHFKNT
                     KEVDSYLQKRNLSYYVRSYGNGFTWKFAPSKRFHSVFKVGSAVESDHFFLGEYVDFDL
                     RLNAIMLREDMGVAIADFYDINLGGEYVYANVDLKGRLPIATNTATNNPVFPDDFTIT
                     DFTIRNLYYSHWAGYLQNVFTVKPVKWTVGARYDAHRDVSEFAYVSPRSSLEVSVTDA
                     DRVSFATGLYQQAHDIYYTNRIFGNPSLTTAKAAHYVLSYHRDLAEKTTFSLETYYKK
                     LWDLAVTGASDTLFSDRGTGYVYGGEILLQRKLTRGFFGWFSYGYSVSRRDDLDGKGE
                     YAFQYDRTHIVNIIASYKLRDWFQAGIKWRYATGLPYTEITGSIYNPMKSEYLPEYSS
                     DYNGRRYPDYQKLDIRFDFYTAWFGLRWNLYLEILNAYNHHNVLSQDFRRHTPYGDNN
                     PRYNYDLPFLPYFGVEVRF"
     gene            7111..7830
                     /locus_tag="PKJ16_06225"
     CDS             7111..7830
                     /locus_tag="PKJ16_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46620.1"
                     /translation="MALLCAALALAGSAVFAQGGAKSDDVTYDKRSVPIQIVKRLSYE
                     NYRDIKLLYTAIVNYGGGEAEFDKLVEEYAEASALFFSNKIVESANMFTRNERNIAAA
                     AYKLAAQYKADVSKLHDDVVRMKVKAGIKMSLKGAAMHGALDKVVADASHAFQRANDL
                     EVRKRPIDAIFYYRMAKRNYFQGYDIVNGQFVELATKTSNKNDKDYLLKLAESYKIPE
                     KYKKDAVDNGNRVYESREKQK"
     gene            8051..9013
                     /locus_tag="PKJ16_06230"
     CDS             8051..9013
                     /locus_tag="PKJ16_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HNV46621.1"
                     /translation="MSIGTVALFVFGIAVLWIGSELIIRNIGPIAKFFGVRELVVTIL
                     GVSVFSSLPELSISALAAANGQADLSLGNVIGSNFVTLTFVTALCALIRPLSINTEIK
                     ERESSWMILSTAMILVLASDGVLSRLDGAVLVLLYAPYIVTVIRDARRNRERDAATEA
                     PARVRVAIAVALEAAGIAGVIVGANLALQSGQSIGAALGIPPLVLGAILFAFGTSLPE
                     MSIALSATFKKKAEISIGEIYASNIFTALAVLGICAIIAPMTITDKRLLSFDIPFMIL
                     AGVIVQIFVTTGSRFNRLEALAILVLYLVFVLQHVLPGGISLSF"
     gene            complement(9192..10466)
                     /locus_tag="PKJ16_06235"
     CDS             complement(9192..10466)
                     /locus_tag="PKJ16_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV46622.1"
                     /translation="MQLPGYYEFCCRVKTISGQKALEKIPQSLQSMKALKPMIITDKG
                     VSGAGLIKLVTAAMKAKGSKVKVASIYDNVPPDSEIKVVEEIAKVYKAKGCDSIIAVG
                     GGSVLDTAKGVNILVSMGSGNLMNYAGAGAVKRKLNPMIAIPTTSGTGSEMTLVAVIA
                     DRARNVKLAFVSYFLLPDIAVLDPRMTKTLPAFLTAPTAMDAMTHAFEAYTCMAKNPL
                     SDTTALAAIQLISQNVLRAIKNPGDLDARLALANGSALAGMSFSNSMVGMVHTIGHAI
                     GGVCHVPHGACMSILLPYGLEYNLHKTAHLTGELLFALAGPEVYAATPKDERAEKAIA
                     YVRQLNQDLHDATKGRHYRFLKEIVDRDGNQMITKDKLPAIARTAMGDASQFYNPEDL
                     DFNDFMMVLEHAYEGTPLDRKKVKKGKKGIKF"
     gene            complement(10515..11054)
                     /locus_tag="PKJ16_06240"
     CDS             complement(10515..11054)
                     /locus_tag="PKJ16_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46623.1"
                     /translation="MNINKYPEIKPGSKLIKRIYVTVLLFVFGRAFQAVAKVDRGAKE
                     EFANMPDDFMLDLCVAPNGPHMIVGKDKNGKIKYFGGNPDGKKITLFMRVKSMDAAIL
                     MFTFQESSCTATSRDRLIVDGDLPVACGVLRLMDLLEVLLLPSPAAKLGVKRYPKWSQ
                     LSPLRKYASRIGTYVRLIA"
     gene            complement(11071..12246)
                     /locus_tag="PKJ16_06245"
     CDS             complement(11071..12246)
                     /locus_tag="PKJ16_06245"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV46624.1"
                     /translation="MQSPHYFEFFSQAKISSGSKALEHIPTELDGFDARKPLVITAKR
                     ATQAGFGKTLVSAFGESNVIIGALFDDAPDYAPIGLIRDLAGLFRDRGCDSIIALGGG
                     AAADIAKGVNILVSERTDDLLQFAGTDTLRNQLKPFVMIPTAVSNGSETSNRATIERR
                     AFVSDFLFPDVITIDPRIVKPRCGDDIAGTAATALVHSVEACITAPGNIVNEAYAQAA
                     IQFITENLRKTLRCSCNKKRNVALANAAAMGGIAFSNAPMGYAHALGTAVADETGLPA
                     GLCMGVIFPYALQHYQQKGAIAPGLLFSVAGIDVFCSTPDRERADRAVSLLLELMSDL
                     RKYLPATLAELHVPRYQLDSAVRAAVAMSGKALAAAECERIIMHAFTGAPQGAGKSK"
     gene            complement(12268..14142)
                     /locus_tag="PKJ16_06250"
     CDS             complement(12268..14142)
                     /locus_tag="PKJ16_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNV46625.1"
                     /translation="MQEIIGTSNRVLEVDLTKQQSKVFEISSTDRTLYMGGKGLGMKI
                     LFDRLPVGVDPLGEENMIVFMPGVLMGTGAPCSGRFEAVTKSPLTGIMASASCGGPFG
                     MALKTSGWDGLLITGSSKNPVYLVIDSKGVQFKNAKGLWGKDTQASQAALEKEGSGAL
                     CIGPAGERMVRFANICSGHRFLGRGGMGAVLGSKNLKGIVAKGKEYKIVPANEKFFDK
                     TRKKFNKYINQNSITSGSYRNFGTNANVNLSNAAWILPVRNFTGGSHGEAHKISGETI
                     REKHDTKYHTCKPCTILCGHKGAFAGKERSVPEYETTGLLGSNLGIFDTVAISDFNEV
                     CTLMGMDTISAGGTIAWAMEATEKGLFSSNLKFGSAPEVTQTLKDIALTRGIGKELAM
                     GSRWLAKKYGGEEFAIHVKGMEMAAYDPRGCYGHGLSYAVANRGACHLSTSLFTLEAY
                     FDMASPYAKRNKHTMVKFFENVYAAMNSLHICHFTAFPVFLEPPLIRFTPVPGLKLLT
                     QNLTPLALALMDVSLWPELWASVLGKTYIPLVSMRKFLAIGERIHVLERYMNTREGIS
                     KKDDTLPGRLLNEGRKCDPLERTVPLEPMLRKYYKARGYDENGIPTKRTLKRLKIEQR
                     "
     gene            complement(14386..14583)
                     /locus_tag="PKJ16_06255"
     CDS             complement(14386..14583)
                     /locus_tag="PKJ16_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46626.1"
                     /translation="MKLLPIAVAIACAVSVCCKQKPAKNTVRLEQIRIDNSNDYRYIF
                     RDEIEREKWRDKRYDKGPFAQ"
     gene            14744..15811
                     /locus_tag="PKJ16_06260"
     CDS             14744..15811
                     /locus_tag="PKJ16_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HNV46627.1"
                     /translation="MVYPVILAGGAGTRLWPLSREDKPKQFHDLTGNGTLFEETVRRL
                     KPIRPGATVVVTGDRYADLTREELKRMKHKGAVLTEPMPRNTAPAILFAATYLEKLGH
                     DSVMIVLPADHHIQKKDVFVATIRKAIETARRDSLVTIGIKPTYPETGYGYIKAQKGS
                     GEVLGIDRFVEKPNVETAQQYLDEGNYFWNSGIFIWKTSVILRWFERLMPECYERFAP
                     LRKLTAQKLLSRDPKVAAIKKRVFSSIEPVSIDYGIMERAENRAVIPSQFGWADLGSW
                     QSIDDILPHDAKKNRTPERSGAIFIDSENCSVVSENLRVALIGLSNVFVVQDGDNILV
                     MDKAASQDVRKVVDVIKKKKR"
     gene            15808..17112
                     /gene="hisD"
                     /locus_tag="PKJ16_06265"
     CDS             15808..17112
                     /gene="hisD"
                     /locus_tag="PKJ16_06265"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HNV46628.1"
                     /translation="MIRLRRRQDLSPAELDALFNRFGDDYSALMVDTVIPIVTDVRKN
                     GDAAVLRYTEKFDTVRLDAVVASSAEIDRGAASVDPALLEAFSAARDNITEFHAHQKR
                     ESIIYSRPDGTTLGVMYQPIERAAVYVPGGTACYPSSVLMGVIPAQIAGTRDITLITP
                     PDRTGSVPPIVCAVARMLGIARIVKSGGAQGIAAAGLGTESVPRAHIIVGPGNIYVTA
                     AKSYLFSLGAVQIDSMAGPSDVLVIADAGAPASWVAADLLSQAEHDPRAAAILVTDSE
                     PLAREVQRLIDADLAANTGRADIKRKAIADNSLIVLVDSIDEAIAFSNDYAPEHMELM
                     VDHPFEHLGAIRNVGSLFLGPHAPVPVGDYFSGTNHILPTGGAARFSSGLSVETFLRR
                     TTFQHLTADALKRAEGPVRLMAEHEGFGEKHGGAVRVRFAPR"
BASE COUNT         3199 a         5393 c         5268 g         3331 t
ORIGIN      
        1 ccgatgaagg gacgcagctc ctcgtaggaa tccgtgccga ttcgcccctt cagctcatgg
       61 tagagcgcgt ggatgaaatc cgtaagcagg atcagcgaaa acccggggtc ctcccgctgc
      121 aggtcgaacc ggccgttctc gccggcattg gttttttcca caaccagcgg cgacgcggcg
      181 acgccatcag caccgcccgc gccccggtgc gccgatctga gaccgtcgac gaccagcgcg
      241 accgccgtca cggccatgga cgcgtagagg aacccgaacg gaatggaaaa gttccgcatg
      301 gcggcccagg cgagcagcca taccgcggcg ttgatggccc ccaccggcgc caggacgccc
      361 ttcagatatc cggaggagcg gtccatctta aaaatgccca gcgccgtgat gccgatcccg
      421 acgaccagca gaaatacggt cgccgcgatg ccgaaccagc ggaacgaatc gaaatcggcc
      481 tgtgtgggcc tgggctggtc tcgtaaccag tccgacctct cgcgttcgcg atcccgcgta
      541 tcccgctcac gcgcgggcgc gccgtcgacg gccttttcgg gcccgtccgg cgtgaccgcg
      601 cgcggcgatt tccgttccgc gtcggcgctc ttgtacgagg agccgccgcc cacgcgcgcg
      661 tgggcggtat tagattggaa tgcgatgatg gcgctcagga ccatcacggc gcacacggca
      721 cgagatatgc ggcgcactgc aatcttcatg gcgtctcctt ggcatgggac tcaattgggt
      781 gtcggcttgg tccgccgatg tttgcatggc gcgcgatctc gcaccggacc cggcggtcgg
      841 cggcaggatg gcgtactcag cggatgaaag caagaagcat tttacgccat cgtccgctgc
      901 acgtcattcg agcacaggtg tatgagggcg tgccgcagcg agctcacctc tccgcgcagg
      961 tccaggttct cgcggaacat cgaaatgaac agcgtgtaga gcatggacgg gtgcatctgg
     1021 tagagcttcc cctcgctggt ctccacggcc gtggtgcagc cgccgaacca ggggctgtag
     1081 tagcggcacc cgtagcaggt ccgctcctcg ccggtggcgt ggcagcagat gtccgagtcg
     1141 aagcgatagt acagcttcgg gtcgggcgcg tcctcgatgt agattgatac gtgtccttcc
     1201 atgacgggcg tggacccgtg gggcagttcg agcgcgaggt tcacttttat cataccgggc
     1261 ctccttcgta tatcccgatc gtccggcttc atgcgatgaa accggccggt gggcggcgga
     1321 cccgaggatc ggccgtccgt gatacagtat agcgcgaagg agtactccgt tcttgcgtgc
     1381 tacgtttgat atcggccttt tccggaaatt acaaagccga atttacaaaa atttacctgc
     1441 gcgaaacgcg ctacgcggat gcgctaaggt cgataaagtt gaaggagagc ccggtcagct
     1501 tgaggacatt tcggatggac ggcatgggat tggcgatgta aaaggagccc ttggtcgatt
     1561 cgatcttccg cttcgcggca tacagcgcgg cgatcccatt ggaatcgatc gcggtcacgc
     1621 gctccaggtc gatcaccaac gagcggacgc tgccgtccgc caccgtgccc acggtgtcgt
     1681 tgagtttcgt gacattgtag atggtgatct cgccgacgat cccgatcacg gtgtgcggcg
     1741 gctgttcgcg tatggtcaca ttcatgggtg cgcatccggt cgttgatgac atcgacagta
     1801 ccacacgttt cgaatccgtc aagaagaata cggcgcgccg cgtcagtaat cgatgtcgag
     1861 gatgatgagc gtcgcatcat cgtcgtgctc atcgacatgc gcggctccct tccagtcgtc
     1921 cagtgcggcg aagatcgcgt ccgccagccc ctcggggggg agcgcgcggt gctgcgtgat
     1981 gagcccgccg agccgctcgt cgccgaagag ctcgcgctga tggttccgcg tatccgggat
     2041 cccgtcggtg atgaggatga tccgatcgcc ccgctcgacg gcgagggaca gctcccgata
     2101 ctcgtattcg gtcgagaggc ccagcggcgc ccccttgatg tgcaatgcct ccagccggga
     2161 cgaacgcgcg cggtacacca ggagctgctt gtgcccggcg ttggagtagc ggatcgcgcg
     2221 cgcctcgagg tcaatgtaca gatagcaggc ggtgatgaac tgcccgtagg agtggtcggt
     2281 catcacccgg ttgatggtgg ccatgagctc ggccgggcgc gcgatgttgt cgcggtagat
     2341 cgagaaggcc atctcggtct tcgcgccgat gatggcggcc gggatcccgt gcccggagac
     2401 atcggcgatg aggacgccca tgcgcgcgtt gtcgacgggg aagaaatcgt acaggtcgcc
     2461 gccgacaccg acggcggggc gatagcgaac cgccgttcgg atcttgccgc actccggcac
     2521 gcgctgcggg agaacggcct tctggattgc ggtcgcgatc tccagttggt agcggatcct
     2581 ctccagctgg ccgtgttcgt ggacagagtc ccgaagggcg atgaggtttt tcacacgcgc
     2641 aataagctca tcgcgctgca cgggcttcgt gaggtaatcg ttcgcgccgg cctcgatgcc
     2701 cgtcacgacg tcctccgggc ggttcttcgc ggtgagcatc acgatgggca gctcgaacat
     2761 cgtgtgcgag cgccgcacca gccggcacaa ctcgtagccc gacatgaccg gcagcatcac
     2821 gtcgacgagc atgaggtccg gcgtctctcc ctcctcgatg cgctgatagg cctcgacgcc
     2881 gtcgtgatag ctcgcgatgc ggtacccctc gagcgcgagg tggttgatga tgacctgcag
     2941 gttcaccgga tcgtcgtcga tgaccatgat cgttttcccg gtatcttccg cgccggggac
     3001 gggccggcga atgtcggcgt caagcggcgc cgcttcggca agggccgcga cgggcggtgc
     3061 aagcgcatca tcgacgctct tcgccgcgac gggcagcgtt acgataaatg tcgcgccccg
     3121 ccccggctcg gattcgatcc ggatcgcgcc gccgtggagc tccaccaggt gtttcacgat
     3181 ggcaagaccc agcccggcgc cgtcctgacg acgcgaggtc gtgccgtcgg cctgacggaa
     3241 ggcgtcgaag atgaccgact gctgctcggg cgcgatgccg atcccggtat cggccacgga
     3301 tatggcgatg cgggacggat cggcgcgatc gggcgccgcc gagacggtca cgctcccctc
     3361 gtcggtgaac ttcacggcgt tcccgacgac attcaacagg atctgctgga ggcggttttc
     3421 gtcggccatg atcggcggaa gcgacgggga cacgtcgttc accaggacga ggcccttgcg
     3481 cacggcgagc ggccgcagga tcgagatcac gaactgtgcg gcattgaaca ggtcaagcgg
     3541 cgccggcagg atcacgaaat cgttgttgcg cagcttcgag aaatcgagga tgtcccccac
     3601 cagccgattg aggcgtcggc cgcttgcgat gatgaggtcc aggtcgtggc gcagatcatc
     3661 ggagaccggc ccggcggcgc cggcgaccag cgattcggcg atgccgatga tcccgttgag
     3721 cggcgtgcgc agctcgtggg tcgtgttggc gaggaactcg tccttgagcc ggtccagttc
     3781 cgtgagtttt cgattggtgc gctccagctc gtcgattttc tgggtgatga tccccgacat
     3841 gtggttgaac gccgcggaga gcgtggcgag ctcgtcggag ccgccggcgc gcaccggcgt
     3901 cgtgtagtcg ccgcgttcga tgcgctcggc cgcgcgcgtg aggcgcatca ccggcgcggc
     3961 aatcacgttg cccagcacga tccccagcat caccagtacg aagaccgcgg cccccagcgc
     4021 cgtggtgatg atccgccgca tccgggcgac cgggcgcaac acctcgtcct tcgagaacac
     4081 caaccgtccc acgcacagcg gcggccggtt gcgcgggccg gcgcccgatg cgaacacgta
     4141 gcaggtcccc gccaccccgt cgatctgcgt ttcgcgaacg cttccgccgt ccaggagccc
     4201 tgcggtctcc tccggggaaa ttcggccgcg ggaaaaatac ggcaacgcgg cgtggtcggc
     4261 ccgggtcttt ttcgacagca ccgcgccatt gatctccggc cgcgacgaga tcatcacgat
     4321 atcggcgtac agcagaccgt cgtcgcgcag gtgcgcgaac gtggcgatca tctccgtcgc
     4381 gtcaacgcgc gccatgtcga ttcgcccggc gttcatgagg aggatggtga gcgcagattg
     4441 cgcgaggatg cggctcaccg tggtcccgtg atggtgcagc agcgcaatgt gctcgtgctc
     4501 ctgctgattg atgatgaaca gcgagagcgg cgtcgcgatc gcgatcaatg cgcccagaaa
     4561 gatgccgatg aacttgtatt ttattttcat tgcggccggt tattttattt caaaatacgg
     4621 gcgaagctcg cagtatggat agtatgatcc gcgccgatgc gtcggtcaag tacgatattg
     4681 acgacgccgc gctcacaaca gacggggaat tccgagagaa ctgcgatgcg ccgttacgcg
     4741 cccataccgg tccgatccct tcgtgcgctc gccatcgcat tcggcattct cgcgtcggcg
     4801 gcgatcccgc agcgcgcgct ccatgcggcc atcgccattc agggggtggt cgtggaccgc
     4861 gcgacgaacc gcccgtccgg cggcgcgacc gtgggcatca tcgagaccgg acagaccgcg
     4921 atcgccgacg agaagggccg cttccggatc gcgctccccc gacccggccg ctatacgctg
     4981 gccgcgcaca gccccggcta cgagcagacg cgcctggtca tcaccgccga cccgcgaaag
     5041 acatacatgg tctatctcac ccagcgggtc atcaccaccc gcgagatcgt ggtgaccgcg
     5101 aaaaaggaga aacagaacgt gagccgccgc acgctcgagc gcgaggagat caaaaaggtc
     5161 cccggcggcg cgggcgacct catgcgctca attcagacgc ttcccggagt ggcgtcggcc
     5221 ggggacatct ccggacagct gtacgtgcgc gggagcggac cgtacgacaa ccgcatcctc
     5281 atcgacaagt tctggctggc gaacgcctac cactacggtg gtttcgtctc ggtcatcaac
     5341 accgacctca tcgactcgat cgatttctat agcggcgcct tcccggccat cttcggcgag
     5401 gcgaccggct ccgtgttgga gctgcacacg cgcgagaagg aggagcccac ctggggcggg
     5461 aagatcaacg tgaacctcct cacggccgac gccgtgctcg agatgccgct ctccgaaaac
     5521 ggctacctca tcctgtccgg ccggcgcagc tacttcgacc tatacgccga gaagttcctc
     5581 agcaacatgg accaggtgcg cgtgacggag atgcccgtct tctgggacta tcaggcgaag
     5641 gccggctacc agttctccaa gggaaaccgc atcgaattcc tctcgtacgg tttcgaggac
     5701 caggtgggcg tccatttcaa gaacacgaaa gaggtcgatt cctatctgca gaaacggaac
     5761 ttgagctact atgtccggtc atatggaaac ggcttcacct ggaagttcgc gccgtcgaaa
     5821 cggttccact cggtcttcaa ggtcggctcc gcggtcgaga gtgaccattt ctttctgggg
     5881 gaatatgtcg atttcgatct gcggctcaac gccatcatgc tgcgcgagga catgggggtt
     5941 gccatcgccg acttctacga catcaacctg ggcggcgagt atgtgtacgc aaacgtggac
     6001 ctcaaggggc gccttcccat cgccaccaat accgcgacga acaatcccgt gtttcccgac
     6061 gatttcacga tcaccgactt caccattcgt aatctgtact actcccactg ggcgggctac
     6121 ctgcagaacg tgttcaccgt gaaaccggtc aagtggaccg tcggcgcccg ctacgatgcg
     6181 caccgcgatg tgagcgagtt cgcgtatgtc agcccgcgct cctcgctcga ggtctccgta
     6241 accgacgcgg accgcgtctc gttcgcaacc ggtctgtacc agcaagcgca cgacatctac
     6301 tacaccaacc ggatcttcgg aaacccgagc ctcaccaccg cgaaggcggc gcactacgtc
     6361 ctctcctatc accgggacct ggctgagaaa acgaccttct cgctggagac gtactacaaa
     6421 aaactctggg acctggccgt caccggcgcc tcagatacgc tcttttcaga ccggggtacc
     6481 ggctacgtgt acgggggcga gatcctgctg cagcggaagc tcacccgtgg atttttcggc
     6541 tggttcagct acggctattc ggtctcgcgg cgggacgatc tcgacgggaa gggcgagtac
     6601 gcgtttcaat acgaccggac gcacatcgta aacatcatcg ccagctacaa gctcagggac
     6661 tggttccagg ccggcatcaa gtggcgttac gcgaccgggc tcccgtacac cgagatcacc
     6721 ggttcgatat acaatccgat gaagagcgag tatctcccgg agtactcatc ggactacaac
     6781 ggacgacgct accccgatta ccagaagctc gatatccgct tcgatttcta caccgcctgg
     6841 ttcggtctgc gctggaacct ctacctcgag atcctgaacg cctacaacca tcacaatgtc
     6901 ctctcgcagg actttcgacg ccatacgccg tacggcgaca acaatccccg ctacaattac
     6961 gaccttccct tcctccccta cttcggggtc gaagtgcgct tctagcatat ttttcttgac
     7021 tttaacaatt agcgctttta cactacccca aacagattgg tatccgctga taccgtataa
     7081 tggagtcacg ctatggtaaa gggatttcgg atggcactac tatgcgcggc gctggcgctt
     7141 gccggttccg ccgtgttcgc ccagggaggg gcaaaaagcg atgatgtcac ctacgacaag
     7201 cgctcggtcc ccatccagat cgtgaagcgc ctgagctatg agaattaccg cgacatcaag
     7261 ctcctgtaca cggccatcgt taattacggc ggcggtgagg cggagttcga taagctggtc
     7321 gaggaatacg ccgaggccag cgcattgttc ttctccaata agatcgtcga atcggcgaac
     7381 atgttcaccc gaaacgagcg caacatcgcg gcggccgcct ataagctcgc ggcgcagtac
     7441 aaggccgatg tgagcaagct tcacgacgac gtcgtcagga tgaaagtgaa ggccggcatc
     7501 aagatgtccc tcaagggggc cgccatgcac ggcgcccttg acaaggtcgt ggccgacgcc
     7561 tcgcacgcct tccagcgcgc gaacgacctc gaggtccgca agcgccccat cgacgcgatc
     7621 ttctactatc gcatggccaa gcgaaattat ttccagggct acgatatcgt caatggccag
     7681 ttcgtggagc tcgccaccaa gacgtcgaat aaaaacgaca aggattatct ccttaagctc
     7741 gccgaatcct acaagatacc cgaaaaatac aagaaggacg cggtggacaa cggcaaccgc
     7801 gtctacgagt cgcgggaaaa acagaagtag cattacacca ggcattccgt atcgcgttgg
     7861 gatgggccgc caatatcgac ggcccgtttt gcgccgtggg gcctgagacg cgcggtcacg
     7921 acggtggcgc gcgggcacga gcgagaggtc ttcaccgaca tgcccgctgc cgggcgcctt
     7981 cggtggcata ccgtctcaca tgagtgcgcg gtacgaatgc tcgatttgac atcgcacgga
     8041 gggctgcgca atgagcatcg gaaccgtcgc gctattcgtt ttcggcatcg ccgtgttatg
     8101 gattggctca gaacttatta tccgcaatat cggccctatc gcgaaatttt tcggcgtccg
     8161 ggagctcgtc gtcactattc tgggcgtatc ggtgttctcg tcgctgccgg agctttcgat
     8221 atcggcgctc gccgcggcga acggacaggc cgacttgtcg ctgggtaacg tcatcggatc
     8281 gaatttcgtc acgctgacgt tcgtcaccgc gctctgtgcg ctcattcgcc cgctctccat
     8341 caacacggag atcaaggagc gggaatcgtc atggatgatc ctctccacgg ccatgattct
     8401 cgtactggcc tcagacgggg ttctttcgcg cctcgacgga gcggtcctcg tgctcctgta
     8461 tgccccgtat atcgtaacgg tgatacgcga tgcgaggaga aatagggagc gcgatgccgc
     8521 aaccgaagcg cccgcccggg tgcgcgtcgc gatcgccgtg gcgctcgagg cggcggggat
     8581 cgccggggtg atcgtcgggg cgaacctcgc cctgcaaagc gggcagtcta tcggcgccgc
     8641 gctgggcatc cccccgctcg tcctgggcgc catcctcttc gccttcggca cctccctgcc
     8701 cgagatgtcg atcgctcttt cggcgacatt caagaaaaag gccgagatat cgatcggcga
     8761 aatatacgcg tccaacatct ttaccgcgct tgccgtcctc ggcatctgcg ccatcatcgc
     8821 gcctatgacc atcacggaca agcgcctgct ctctttcgac atcccgttta tgattctggc
     8881 cggcgtcatc gtacagatat tcgtcaccac cggctctcgg ttcaaccgcc tggaggccct
     8941 ggcgatcctc gtgctctacc tggttttcgt cctacagcat gtcctgcccg gcgggatttc
     9001 cctgtcgttc tagcgttatc ggcccacatc acgctaaaag cgacagcgcg atcttttcga
     9061 acaatgacgg gacatatcct cgccatgctc taaaataaaa acgcgggtga atagctcacc
     9121 cgcgttttgt gtcgagacgt catcgtaccg gtcggttccg tgctccgtat ctgacgccgt
     9181 ggtcccttct gctaaaactt gattcccttc ttccctttct tgaccttctt ccggtccagc
     9241 ggagtcccct cgtaggcgtg ctctagaacc atcatgaaat cgttgaagtc caggtcctcg
     9301 ggattgtaga actgcgatgc gtcgcccatt gcggtgcgcg cgatggccgg cagcttgtcc
     9361 ttggtgatca tctgattgcc gtcgcggtcc acgatctcct tgaggaagcg ataatggcgc
     9421 cccttggtcg catcgtgcag gtcctgattg agctgtcgga cataggcgat cgccttctcg
     9481 gcgcgctcgt ccttgggggt cgcggcgtac acctcgggcc cggccagcgc gaagagcagt
     9541 tcgccggtaa ggtgcgcggt cttgtgcagg ttgtattcca gcccgtaggg cagcaggatg
     9601 ctcatgcagg ccccgtgggg gacgtggcac accccgccga tcgcgtggcc gatggtgtgg
     9661 accatgccca ccattgagtt cgagaagctc atccccgcaa gcgccgagcc gttggcgagt
     9721 gcgaggcgcg cgtcgagatc gcccgggttc ttgatcgccc gcagcacgtt ctggctgatc
     9781 agctggatcg ccgcgagcgc cgtggtgtcc gagagcgggt ttttcgccat gcaggtgtag
     9841 gcctcgaagg cgtgcgtcat ggcgtccatg gccgtgggcg cggtgaggaa ggccggtagg
     9901 gtcttggtca tccgcggatc gagcacggcg atgtcgggca gcagaaagta cgatacgaag
     9961 gccagcttca cattgcgcgc ccggtcggcg atcacggcga cgagcgtcat ctccgatcca
    10021 gtcccggagg tggtggggat ggcgatcatc gggttgagct tgcgcttcac tgccccggcg
    10081 ccggcgtagt tcatgaggtt cccgcttccc atcgaaacga gaatattcac tcccttggcg
    10141 gtgtccagca ccgacccgcc gccgacggcg atgatggaat cgcagccctt ggccttgtac
    10201 actttggcga tctcctcgac caccttgatc tcggagtccg gcggaacatt gtcgtaaatg
    10261 gaggcgacct tcaccttcga gcccttcgcc ttcatggcgg ccgtcacgag cttgatgagc
    10321 ccggcgccgg agacgccctt gtcggtaatg atcatcggtt tcagggcctt catgctctgg
    10381 agcgattggg gaatcttctc gagcgccttc tggccggaaa tcgttttaac acggcagcaa
    10441 aattcataat atcctggcag ttgcatgatc tactcctctc gaataaaatc tacacgatgg
    10501 tcacggcacg gtccctaggc gatgagccgg acataggtcc cgatgcggct ggcgtatttc
    10561 cgcagcggcg agagctgcga ccacttggga tagcgcttca cgccgagctt cgccgcgggc
    10621 gacggcagca ggagcacctc caggaggtcc atcagccgca gcaccccgca ggcgaccggc
    10681 aggtcgccgt cgacgatgag tcggtcgcgc gaagtcgccg tgcaggagga ctcctggaac
    10741 gtgaacatca gaatggccgc atccatgctc ttcacgcgca tgaacagcgt gatcttcttg
    10801 ccgtcggggt tgccgccgaa atacttgatt tttccgttct tgtccttgcc cacgatcatg
    10861 tgcgggccgt tgggcgcgac gcacaggtcc agcatgaagt cgtcgggcat gttcgcgaac
    10921 tcctccttcg ccccccggtc caccttggcc accgcctgga aggcgcgccc gaagacgaac
    10981 agcagaacgg tcacgtagat gcgcttgatg agctttgaac ccggctttat ctcgggatac
    11041 ttgttgatat tcattgccac ttcctcgctg ttatttcgat ttccccgcgc cctgcggcgc
    11101 gcccgtaaag gcatgcatga tgatgcgctc gcactcggca gccgcgagcg cttttccgct
    11161 catggcgacg gccgcgcgca ccgcgctgtc caactgatac cgcggaacat gcagctcggc
    11221 gagcgtcgcc ggcagatact tccgcagatc ggacatcagt tcgagcagca gcgaaacggc
    11281 ccggtcggcg cgctcacggt ccggggtcga gcagaaaacg tcgatgccgg cgacggagaa
    11341 cagcagtccc ggcgcgatgg cgcccttctg ctggtagtgc tgcagcgcat aggggaagat
    11401 cacccccatg cacaggcccg ccggaaggcc ggtctcgtcg gccacggcgg tacccagcgc
    11461 gtgggcgtat cccatcggcg cattggagaa ggcgatcccg cccatcgcgg ccgcattggc
    11521 gagcgcgacg ttccgctttt tgttgcagct gcaccggagg gtcttgcgca ggttctcggt
    11581 gataaactgg atggccgcct gggcataggc ctcgtttacg atgttgcccg gcgcggtgat
    11641 gcaggcctcc acggagtgga ccagcgccgt cgccgcggtc cccgcgatat cgtccccgca
    11701 gcgcggtttc acgatccgcg gatcgatggt gatgacatcc ggaaacagaa aatccgagac
    11761 gaacgcccgc cgctcgatgg tggcgcggtt cgaggtctcg gagccgttcg agacggcggt
    11821 ggggatcatc acgaagggct tgagctgatt gcgcagcgta tcggtcccgg cgaattgcag
    11881 gagatcgtcc gtgcgctccg acacgagaat gttcacgccc ttggcgatgt cggccgccgc
    11941 gccgcccccc agggcgatga tggaatcgca gccgcgatcg cggaagagcc ccgccaggtc
    12001 gcggatgagt ccgatgggcg cgtagtcggg cgcatcgtcg aacagcgccc cgatgatcac
    12061 gttggattcg ccgaaggccg ataccagcgt tttaccgaat ccggcttggg tcgcccgttt
    12121 cgcggtgatg accagcggct tgcgggcgtc gaagccgtcc agctccgtag ggatatgctc
    12181 gagcgccttg gatcccgatg atatcttcgc ctgactgaag aattcgaagt agtgtggtga
    12241 ttgcatgata tgctcctgat gatcgcctta tcgttgttct atcttaagcc gcttcaaggt
    12301 gcgcttcgtg ggaatgccgt tttcgtcgta tccacgcgcc ttgtagtact tccgcagcat
    12361 gggctcgagc ggaaccgtgc gctccagcgg atcgcacttg cgcccctcgt tgagcagccg
    12421 tccgggaagg gtatcgtcct ttttcgagat gccctcccgg gtgttcatat aacgctcgag
    12481 cacgtgaatg cgctcgccga tcgcaaggaa cttgcgcatc gacaccagcg ggatgtaggt
    12541 ctttcccaat accgaggccc agagctcggg ccagaggctc acgtccataa gcgccagcgc
    12601 cagcggcgtg aggttctgcg tgaggagctt gaggcccggc accggcgtga agcggatgag
    12661 cggcggctcc aggaacaccg ggaaggccgt gaagtggcat atgtgcagcg agttcatcgc
    12721 cgcatacacg ttctcgaaga acttgaccat ggtgtgttta ttgcgcttcg cataggggct
    12781 ggccatgtcg aagtaggcct ccagggtaaa cagcgacgtc gagaggtggc aagcgccgcg
    12841 attggccacc gcataggaga gaccgtggcc gtagcaaccg cgcggatcgt aggccgccat
    12901 ctccatcccc ttgacgtgga tcgcgaattc ctcgccgccg tacttcttcg caagccagcg
    12961 cgatcccatc gcgagctcct tgccgatgcc ccgcgtgagg gcgatgtcct tgagcgtctg
    13021 cgtcacttcg ggtgcggagc cgaatttcag attcgacgag aacaggccct tctcggtggc
    13081 ctccatggcc caggcgatgg tcccgccggc ggagatggtg tccatgccca tgagcgtgca
    13141 cacctcgttg aaatcggaga tcgcgaccgt gtcgaagatg cccaggttgg accccagaag
    13201 ccccgtcgtt tcatattccg gaaccgaccg ctccttgccg gcgaacgcgc ccttgtgccc
    13261 gcacaggatg gtgcagggct tgcaagtgtg gtacttggtg tcgtgcttct cgcggatggt
    13321 ctcgcccgag atcttgtgcg cctcgccgtg cgagccgccg gtgaagttgc gcaccggcag
    13381 gatccacgcc gcattcgaga ggttcacgtt tgcgttggtc ccgaaattcc ggtatgaccc
    13441 ggaggtgatg ctgttctggt tgatatactt gttgaatttc ttgcgggttt tatcgaagaa
    13501 tttttcgttc gcgggcacga tcttgtactc cttgcccttc gcgacgatgc ccttcaggtt
    13561 tttagagccc agtaccgcgc ccatgccgcc gcggcccagg aagcggtgcc cggagcagat
    13621 gttcgcgaag cggaccatgc gctcgccggc cggcccgatg cacagcgcgc cgctgccctc
    13681 tttctcaagc gccgcctgtg acgcctgcgt gtccttgccc cagagcccct tggcgttttt
    13741 gaattgcacg cccttggaat cgatcaccag atagaccgga ttcttcgacg agccggtaat
    13801 cagcagcccg tcccagccgg atgtcttgag cgccatgcca aagggtccgc cgcaggaggc
    13861 cgaggccatg atgccggtga gcggcgactt ggtgaccgcc tcgaaacggc ccgagcaggg
    13921 ggcccctgtc cccataagaa cgccgggcat gaacacgatc atgttctcct cgccaagcgg
    13981 atcgaccccc acggggaggc ggtcgaacag gatcttcatc cccagacctt ttccgcccat
    14041 gtacagggtg cggtcggtgc tgctgatctc aaagacttta ctctgctgtt ttgtgaggtc
    14101 gacctccaaa accctgttac tggttccgat gatttcttgc atagagcttc tccttacatg
    14161 atgactgcgg tgtgaatgcg cggctgtctc tttacgaaca ggtctcacga tcgaacgccg
    14221 tcggaggatg cgagcaccag ggacgacgaa tcaatttgtt tgtatccgtg atgtttgaac
    14281 tgtaaagcgg tgatgttttt gttcaatcaa aatactggtt tgtaaaaaaa ttttttcaaa
    14341 aatctgttgc gtttccaagg aaagattgtc tcgcggtcaa atttttcact gggcgaacgg
    14401 gcccttgtca taccgcttgt cccgccactt ctcccgttcg atctcgtcgc ggaagatgta
    14461 gcgatagtcg ttggagttgt cgatccggat ctgctcgaga cggacggtgt tcttcgccgg
    14521 cttttgtttg cagcacacgg atacggcgca ggcgatggca acggcaatcg gcagcagctt
    14581 cataacggca gtcctccttc gatactaata tatcgggccg tcgcggcgcg aacaagcgtt
    14641 ttatccgcga aattctttat tgacataacc gggacgctat ttttgattac tcatcctcta
    14701 gggagcggga gaggaccact caacagcgac tggtgatacg attatggttt atccggtaat
    14761 cctggccggc ggggccggaa cgcggctgtg gcctctcagc cgggaagaca agcccaagca
    14821 atttcatgac ctcacgggaa atggcaccct gttcgaagag accgtcaggc ggctcaagcc
    14881 gattcggccg ggcgcgaccg tcgtagtcac cggcgaccgc tacgcagacc tcacgcgcga
    14941 ggagctcaag cgaatgaagc acaagggcgc ggtcctcacc gaaccgatgc cccgcaacac
    15001 cgccccggcg atcctcttcg ccgcgaccta ccttgagaag ctcgggcacg actccgtcat
    15061 gatcgttctc ccggccgacc accacatcca gaagaaggac gtattcgtcg ccaccatccg
    15121 gaaggcgatc gagacggcgc gacgggactc gttggtcacc atcggcatca agccgaccta
    15181 tccggagacc ggctacggct acatcaaggc gcagaaggga agcggcgagg tcctggggat
    15241 cgaccgcttc gtcgaaaaac cgaacgtgga aacggcgcag caatacctcg acgagggcaa
    15301 ttacttctgg aacagcggca tcttcatctg gaaaacctcg gtgatcctcc gctggttcga
    15361 gcgcctcatg cccgagtgct acgaacgctt cgcgccgctt cgcaagctca ccgctcaaaa
    15421 gctgctgtca cgagacccca aggtcgccgc gatcaaaaag cgggtcttct cctccatcga
    15481 gccggtctcc atcgattacg gcatcatgga gcgcgcagaa aaccgcgcgg tcattccctc
    15541 acaattcggc tgggccgatc tgggcagctg gcagtcgatc gacgacatcc tcccgcacga
    15601 cgcgaagaag aaccgcaccc ccgagcgcag cggcgcgatc ttcatcgact cggaaaactg
    15661 ctcggtggtg tccgaaaacc tgcgcgtcgc cctcatcggc ctttcaaacg tcttcgtggt
    15721 gcaggacggc gacaatatcc tcgtcatgga caaggcggcc tcccaggacg tgcgcaaggt
    15781 ggtcgacgtc attaaaaaga agaagcgatg atccgtctgc gacggcgcca ggacctctcc
    15841 cccgcggagc tggatgcgct cttcaatcgc ttcggcgacg actacagcgc gctcatggtc
    15901 gataccgtca tccccatcgt caccgacgtc cggaaaaacg gcgatgccgc cgtgctgcgc
    15961 tataccgaga aattcgacac cgtgcggctg gacgcggtgg tcgcatcgtc cgccgagatc
    16021 gaccgcggcg ccgcttcggt cgacccggcg ctcctcgagg cgttttccgc cgcgcgggac
    16081 aacatcacgg aattccacgc ccaccagaaa cgcgagagca tcatctactc gcgcccggac
    16141 ggcaccacgc tcggcgtcat gtatcagccg atcgagcggg cggcggtata cgtccccggc
    16201 ggcacggcgt gctatccctc gtcggtgctt atgggggtga tccccgcgca aatcgcgggc
    16261 acgcgcgaca tcaccctcat caccccgccc gaccgcaccg gttcggtgcc gcccatcgtg
    16321 tgcgccgtgg cgcgcatgct gggcatcgcc cggatcgtga aaagcggcgg cgcgcagggg
    16381 atcgcggcgg cgggcctcgg gaccgaaagc gtcccgcgcg cgcacatcat cgtggggccg
    16441 ggcaatatct acgtgaccgc cgccaagtcg tacctcttta gcctgggcgc ggtgcagatc
    16501 gattccatgg ccggaccgag cgacgtgctg gtgatcgcgg acgcaggcgc gcccgcctcc
    16561 tgggtcgccg ccgatctcct ttcgcaggcc gagcacgatc cgcgcgccgc cgccatcctg
    16621 gtgaccgact cggagccgct cgcgcgggag gtccaacggc tcatcgacgc cgatctcgcg
    16681 gccaacaccg gccgcgccga tatcaagcgg aaggccatcg ccgacaacag cctcatcgtc
    16741 ctcgtcgact cgatcgacga ggccattgcc ttttcgaacg actacgcgcc ggagcacatg
    16801 gagctcatgg tggaccaccc cttcgagcat cttggcgcta tccgcaatgt gggatcgctc
    16861 ttccttgggc cgcatgcgcc ggtccccgtc ggcgattact tttcggggac caaccatatc
    16921 ctgcccacgg ggggcgcggc gcgcttctca tcgggcctct cggtggagac ctttttgcgg
    16981 cgcaccacct tccagcacct taccgcggac gcactgaagc gcgccgaggg cccggtgcgc
    17041 ctgatggccg aacacgaggg gttcggcgaa aagcacggcg gagcggtgcg cgttcgcttt
    17101 gcgccccgct agcggttcgt ttatcttcac gggaacacgg gaatcggaca tgttcgaggg
    17161 aaaaacgctg tggaacatca tgcagatggg g
//
LOCUS       DAORHF010000050        17135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2445_length_17135_cov_5.050995, whole genome shotgun sequence.
ACCESSION   DAORHF010000050 DAORHF010000000
VERSION     DAORHF010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 17135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2445_length_17135_cov_5.050995"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PKJ16_06270"
     CDS             complement(<1..1049)
                     /locus_tag="PKJ16_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HNV46629.1"
                     /translation="MDAIYKKIKGQFYNKLLIAIIIGVALSAVSLYFVFVCQQADDGL
                     KIDDRFMALEVRPGSPAAEAGIRTGDRVLAIGGVRLARIGELRTVHESVMHTVSALCG
                     VLVPHRAVPYEIQRGEAVVTVSVVPTRIGLQGLEFSVLFRFTLGLFFLLLGSLVYFRK
                     HTDRLALIFFLYCATQSLYLTMGYYSSFWNCGIIRFLFYAVSPMKVLSGVLFLHFFSA
                     LSSPAEARAGRALRAALYALAGALIVAFAVTLFRSELFTFVYWTRDCFYYLCHGAGVA
                     ALLYQYVRNRANPDYSIKRQQIKIILYGTTVSVALYLAFKLAIIAGAGGGTNIVSENE
                     QILTGLLFSIVPISIG"
     gene            1264..2469
                     /locus_tag="PKJ16_06275"
     CDS             1264..2469
                     /locus_tag="PKJ16_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV46630.1"
                     /translation="MKKAIIALGIIVLVLAGYILYQKFGVSPAINVLDYFIGRVDIIK
                     PGNRIVPAAIKLRLHTKDTVKTHAKSKAFIQCGADNMLELFENTELRMDTLPVTLKSS
                     ADVAALHLMRGKAAVMLEKLTSKGSFSIRTINHTIAVRGTVFSAEITANGAIVMVKEG
                     GVTITETANPKKRIDVDAGSKAIITGTRIETKDMTDGDAATFREIVELRPIAGINCTP
                     PEYIERFFRWRLGLKDRVPAEPSGDEGAEKESTRGEDAAKDRDTPDTPDREAAKKETK
                     DTAKDDKPAATSMARLGVNAFTANGVEQKVAADLTNKLYAKLRASKGVEAVVMRGGGR
                     TSARQLMGRVSKLGATTVLALRVVDGQSGRLVYSRSLNYKDGEDIDGRLDEIAGQIGR
                     KGEIWAGAK"
     gene            complement(2725..3915)
                     /locus_tag="PKJ16_06280"
     CDS             complement(2725..3915)
                     /locus_tag="PKJ16_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HNV46631.1"
                     /translation="MIFLCMPLAPACFIEMERTNPNDPQSDAFSSPLSSAAGQPVFYV
                     MRTGQENSVSLYDDGYYKKGYSWKTPRFINLGDGTVRDNLTGLMWTADANIMKTRDPK
                     FDGDGTAEDGLVTWYHAMDYISKLNGEKYLGYSDWRAPNANELMSLIDFGQAYIYTWL
                     MSEGFSNVPLNARIWSSTTNPDLSYNSAFFVDLITAGGAWMSFKTEFYYIWPVRGENA
                     GSERVLQKTGQTVTYYTNDDGRLRKGEPWPEPRFTDNSNGTITDNLTALVWAKDGNIM
                     VSRNPGYDVDGTANDGRVAFTTALNYIALLNTENYLGFSDWRLPNVREFTSLWNFSYH
                     IATWLQNQGFTNVQNYYITSTGYGAIPIDRSYHIGIYVPRFQAQPKTYSDYFFYVIPV
                     RGGR"
     gene            4181..4822
                     /locus_tag="PKJ16_06285"
     CDS             4181..4822
                     /locus_tag="PKJ16_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46632.1"
                     /translation="MPSSLTASLTALTILLALCSAALAQPKDPEAPRAPVVVDEKAGA
                     KKERAANQPEKRADLYAYSVKALRADGRSVEGTADLASPSITFTHVKGGVSYEKTVRW
                     DDVESIRAVEWKPVGHDKTSAKETQPVFFYPSRWKLVLKSGKLYDFTGQIALFNKIVV
                     KNRDGSAAVYTYFVDYWKRTGTRSGMWENARSTDFDHPRRHPDRSACVQVRFR"
     gene            5101..6267
                     /locus_tag="PKJ16_06290"
     CDS             5101..6267
                     /locus_tag="PKJ16_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46633.1"
                     /translation="MIKRRLARKAKELFTQYPVITITGPRQSGKTTLAKNTFPRLKYF
                     NLEDIETRTFAREDPKGFLKGCAGGAILDEIQNAPDLLSSIQVYVDSLNREGLFVLTG
                     SRQLELMESVSQSLAGRTALLKLLPFSIEELHEHGNILESDEYLYHGFYPRIHDKKLN
                     PSEALADYYTTYVERDLRQLANIHNLHLFHTFVKHCAGRVGQLINLSSLASDTGISHT
                     TAQDWLTLLEASYIIFRLQPYFANIGKRLIKSPKLYFYDVGLAAHLLGIESIQHVAHH
                     PLKGNLYENMAISEILKYRFNRNKTNNLFFFRDARGREVDCLYTISHKIYPIEIKSSR
                     TFNRDFLTSIEYFMDAIHESERGFLVYDGEGDIDIRNITLLSPRSIWEKLDALG"
     gene            complement(6504..7367)
                     /locus_tag="PKJ16_06295"
     CDS             complement(6504..7367)
                     /locus_tag="PKJ16_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HNV46634.1"
                     /translation="MNDHALKGIVLIALSALAFALSTVFAKFVTTQSAISGIEIAFFR
                     FAAGLAVIVPYMLARKKTFRAVEKRYVALRGVSNAVAVIFFYLGIKFTTVTNANMLNM
                     TYPVFVYLMAPFINRERGSWRGWVSVALTIAGSWLIMMPGFAAINAGDVFSLLSAVVA
                     GVAVCALRESRKYDDSEVILFHLMAIGTAVCLVATLPNFVVPQGMMIVHIALTTLFAL
                     VGQVFITEGYRFIDAGPGALVSASRVLFAGVLGAVVFADALTVNLVAGGALIMLSLAG
                     VSLNGGRKEGD"
     gene            7501..7941
                     /locus_tag="PKJ16_06300"
     CDS             7501..7941
                     /locus_tag="PKJ16_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HNV46635.1"
                     /translation="MLVVLDTNVLYQALRNRSGASHFILSLIRENRLSLAISIPVHQE
                     YQKVLSRTASQKDLNLTRTDIDRVLRFIAYIGRPYATYFHFRPNLQDESDNIFVELAL
                     ASNCNYLITSNTRDFTIKAELKFDDLRIITPSNFAATWRKAHES"
     gene            7931..8191
                     /locus_tag="PKJ16_06305"
     CDS             7931..8191
                     /locus_tag="PKJ16_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HNV46636.1"
                     /translation="MKAKSSVLTIRVPRALKTRIERVADEQGVSINQLALYAFTKEIT
                     EMESASYFSSLRKSKSKKQLYDDFDSVMKKVGSVTTPKWDKL"
     gene            complement(8204..9046)
                     /locus_tag="PKJ16_06310"
     CDS             complement(8204..9046)
                     /locus_tag="PKJ16_06310"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV46637.1"
                     /translation="MPSLPSRIVDFHVHLFPDRLFESIWRHFVTDYGWSVIHQLYYRE
                     CVAYLRERGVGPIVYSNYAHRPGIARGLNAWNRAVVDELPDLYCLAAYHPGDDDAFDY
                     AREALAHPKVLGIKLQLLVQRFYPHDERLFPLYELIMERGKRVLLHTGTGPVGNEFVG
                     AAHFERLMRRYPELPVTVAHMGALEYAAFVDFLDAFPNMYLDTAFVFLPQFDGYCDLD
                     AALLEKYRDRILYGSDFPNLIMPREDEIEHLLGLGLSQEFYDAVFYNNGMRLIRGISR
                     MGDV"
     gene            9278..9850
                     /locus_tag="PKJ16_06315"
     CDS             9278..9850
                     /locus_tag="PKJ16_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNV46638.1"
                     /translation="MKKKPDAAPAPKNPIGAKLAAQRRKKKLSLEVLAEKTGLKVRDL
                     EAIESGVVFPPVGDILKISRALTVDPGQLLGSPAKDPDERKKRRIDDFNKRASSYLYT
                     ILTPQAMNKHLRAFRVVIPPGAEHPKISYQHEGEEFIYVLEGTLEITVGRKKHALKQH
                     DALHFDSGITHALKNPGAKECVLVVTVYTP"
     gene            9847..10989
                     /locus_tag="PKJ16_06320"
     CDS             9847..10989
                     /locus_tag="PKJ16_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV46639.1"
                     /translation="MNLPLTQEQIMIRDMIRDFARSEIEPVAQDYNRRGEYPASLVKK
                     LGELGLFGMMVPESYGGSAAGAVSYSLALQEIAFSCASIAVTLSVTNLVAEPMLAFAT
                     EEQKREFLFPLASGEHIGAFALTEPEAGSDPGGLSTSAVKKGGVYVLNGVKQFITNGE
                     YAGVFVVIARTAPGKNGLSAFLVPRGAKGLSVGKEEDKMGLRASNTVEIVLDDCAVPE
                     ANMLGKPGMGLQIALNALDSGRIGIASQATGMIAACLHEAVSYAGERRQFGKPIIKHQ
                     TIQNMIADIAVEHEAAALLVWSAASLKDAKRPFTREASIAKLAATESLNRAAYRALQV
                     FGGYGYIRDNKIERIYRDARVTTIYEGTSEIQRMIIARETEALLSR"
     gene            complement(11069..11806)
                     /locus_tag="PKJ16_06325"
     CDS             complement(11069..11806)
                     /locus_tag="PKJ16_06325"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV46640.1"
                     /translation="MKKCMIGAVIIVLCAAQSAFALTGRQIMEKSDALTKPNTSTSTV
                     VMVIEKGGSTVDKEFELISKKAGPRDMALITFTRPSAIKFLTHSKKGAEDDQWLRLSS
                     GKVKRISSSEKDKSFVNSHLYYEDLSSRNIDDYEYKYLGDKQAAGADCYVVESVKKKG
                     ERVYSKSVLYVRKSDFFVVRIDVYMKGGLHKYLENYEIKTVQGIITPMKAVMTMSDGS
                     GKTTLNVKSVKYNMNLGDARFSKDAMR"
     gene            11935..12567
                     /locus_tag="PKJ16_06330"
     CDS             11935..12567
                     /locus_tag="PKJ16_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV46641.1"
                     /translation="MRKKTAPTTEQAAAPNKERIVSSAKSLFAAKGFAATGLREIAAN
                     AGVSLGNIYNHFQSKEEIFQSVFDSKQIEESLADTFVLIADDFPLNIDKVILSIKRTV
                     DANYELYRIVYIDLIEFGAVNTNRLLENLLSFGQSIFREMLYDKARRGILKELDYDFF
                     ARQFLIAQISFFSSLRVLPAIRMDRYTDEEISKMMANVILNGIRREPNAS"
     gene            12557..12937
                     /locus_tag="PKJ16_06335"
     CDS             12557..12937
                     /locus_tag="PKJ16_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46642.1"
                     /translation="MRRETHMQGLIRSACALALVAGLALVISVIIVPEWRADSRACAS
                     PIRVADDAAAASESPKDTAPTKPTNDPETAPAKDGADHTAPKTSAPDDGAVKDDRGAS
                     GDKQPETTDDAEAAGDEKKKPCLR"
     gene            12925..14259
                     /locus_tag="PKJ16_06340"
     CDS             12925..14259
                     /locus_tag="PKJ16_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46643.1"
                     /translation="MFTMTGFVELENFIGIHKNTESDEPYKKCELRNRTQLRYGTDTA
                     YLLATANLYAMATFLSDRLGREYRYSEKDTVHRNLRISSKYYEASFNELYASYDTDRV
                     RLRAGNQIYGWGTADVYNPTSYFNPFDMREYFFKDEDEQRLGVPSVSAMFFIGDFTLE
                     TVLMPVHVPMALAPSGNFWAIESKLGRQPVILEEGAALDAKPENMGAGARFAGSLMGV
                     DVSLSAYHGPYKEPLLVPDGTRILPNKPLALAVKQQYHVLTMIGTDVSFKMDKFVLQA
                     EAAYSPDKTGMVMQDTDDIRRIAFPFETRKSPYVSYSAGFNYFIPLGRLIEGHEGDTV
                     FTAEWHQSLYLDRELADPLFTKIVSCRLEDSYFGGRLKVSLTAMFDTAKSGYLLWPQL
                     TWDFQNGLSFSVTYAAIEHTGASAGRINENESAFYYLRDNDMVTWRVRYAYK"
     gene            14246..16081
                     /locus_tag="PKJ16_06345"
     CDS             14246..16081
                     /locus_tag="PKJ16_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46644.1"
                     /translation="MRINNGVLMILVAASLITACVNKQEKGPITISGKIDASAVSGSA
                     SGPTLVAVAKTDSIEAIERDPAGAIVMIAGIDEHSGTYELELGEYDVQPGDPVTIIAF
                     IDNAYANGIPYPKRGDFIGFYIEPSSLSTSYALKAGANPNLDIAINREVFSFDAAVSG
                     TVGGTDAGDLIIIAYAGEITSSDMSALDTSAVIGYARASKGAAPTPYRVRIMPYGYNV
                     PIQNVYLIAVLDRNRNGVVDGGDAIGFHAAGANGLPTPITVNEGENAGIDIAFSMTVP
                     VPSGVAMSIKGSFAVPAGYNASSKPMYLIVARIDNPKTLFTDPLAAIKYFYKMPPGEF
                     VFNIDLSKTDLVPGDRVVVATLWDRDNAGGFPYPTRGDKLGLAQNNDTYSYFIELALG
                     VNPVPQEGYEFNTNKLIYDFDSSVKFALDRGAIESYNVDTAQIIILCVHVNGVDIGIK
                     PSGVSVKIDMDYVLGSTVIRPPAFDHITSAPHPILPAEDMRTLGIFRALYQEIVVWEN
                     SVPPDPLIQGVANPSSGETETAYLFAVLDRNGNGNLDSDDVVGYYNREYVVTTGSIVI
                     PGYGSVAVPNGTYYLPATIRTISTGANTNSDGQPYWITFRYYDPP"
     gene            16139..>17135
                     /locus_tag="PKJ16_06350"
     CDS             16139..>17135
                     /locus_tag="PKJ16_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HNV46645.1"
                     /translation="MNKLFDVIIRKYKYIMVAIVLLSLPMGYYFTQYKFFNHIDIFFD
                     ASDPNLQFYKNFQKRYGNEELGVILFKAPDGTVFNERVITVIRSISAQLKEADGVQRV
                     FSLTEAKEAQAEGNTIRFVNIIPKGEIAPTAYDPLKHKVLKNPVVVNSLVSKDGATTA
                     ILFELKPTSDNEKKRELLQRIMAFSLKAAGDAVRLHFAGVPYVEVEMNILSKRDFLVF
                     TPITFFLIFIVVAFMLKRISLALLCQMNLLLTLVWAIGFFSMMGESFNMVTVIMGAIL
                     LAIAVADSIHILAHYREADAYHDNHDDAIRHTLKSIWMPCLLTSLTTAVVRLVSRQ"
BASE COUNT         3806 a         5207 c         4950 g         3172 t
ORIGIN      
        1 ccgatcgaga tcgggacgat cgagaacagg agcccggtga ggatttgctc gttttcggaa
       61 acgatattgg tgccgccccc cgcccccgcg atgatcgcca gcttgaaggc gaggtagagc
      121 gccacgctca ccgtggtccc gtaaaggatg atcttgatct gctggcgctt gatgctgtag
      181 tccgggttcg cgcggttgcg gacatactga tacaggagcg cggccacgcc ggcgccgtgg
      241 cacaggtagt agaagcaatc gcgcgtccag tacacgaatg taaagagctc ggaccggaac
      301 agggtgacgg cgaacgcgac gatgagcgcg ccggcgagtg cgtagagcgc cgcgcgcagc
      361 gcccggcccg cgcgcgcctc cgccggcgag gagagcgccg agaagaaatg gaggaacagc
      421 accccgctga gcaccttcat gggcgagacg gcatagaaca gaaagcggat gatgccgcaa
      481 ttccagaacg acgagtagta ccccatcgtg aggtacagcg attgcgtggc gcagtagagg
      541 aagaagatga gcgccagccg gtcggtgtgc ttgcggaaat agaccaggct ccccagcaac
      601 aggaagaaaa gccccagcgt aaaacggaac aggaccgaga actccagccc ctgcaggccg
      661 atgcgcgtcg ggaccaccga caccgtgacg acggcctcgc cgcgttgtat ctcgtagggc
      721 accgcccggt gcggcacgag gacgccgcag agcgcggaga ccgtgtgcat cacgctctcg
      781 tgcaccgtgc ggagctcgcc gatgcgcgcc aggcgaacgc ccccgatcgc cagcacccgg
      841 tcacccgtgc ggatcccggc ctcggccgcg ggactgccgg gacgcacctc gagggccatg
      901 aaacgatcgt cgatcttcag cccgtcgtcc gcctgctggc acacgaacac gaagtagagc
      961 gagaccgccg acaacgccac cccgatgatg atcgcgatga ggagcttgtt gtagaactgt
     1021 cccttgattt tcttgtagat ggcgtccatg ggcacccgat gtcggatcga ttggcgagcg
     1081 tatcggggcg atggaaatta ttccagcatt tttcgaggaa ttcgattgct ccacgcgaag
     1141 ccccacaact caacaggaac actgaatcag cacacaaaga gtgatttaca attgacaaac
     1201 cgtgagcgaa ccttttagtt catatcggtt cattacagtt tacagaaaat actgggcaat
     1261 ccaatgaaaa aagccatcat cgcactcgga atcatcgtcc tcgtccttgc cggatacatc
     1321 ctgtaccaaa aattcggcgt gtcgcccgcc atcaacgtgc tcgattactt catcggaagg
     1381 gtggacatca tcaagccggg taaccggatc gtccccgcgg cgatcaaact gcgcctccac
     1441 accaaagaca ccgtgaagac gcacgcgaaa tcgaaggcgt tcatccagtg cggcgcggac
     1501 aacatgctcg agctcttcga gaacaccgag ctccggatgg acacgctgcc ggtaacgctg
     1561 aagtcgtcgg ccgacgtggc ggcgcttcac ctcatgcgcg gcaaggcggc cgtcatgctc
     1621 gagaagctca ccagcaaggg atcgttctcg atccggacga tcaatcatac catcgccgtt
     1681 cgcgggaccg tgttctccgc ggagatcacc gcgaacggag cgatcgtcat ggtgaaggag
     1741 ggcggcgtaa ccataaccga aaccgcgaat ccgaagaaac ggatcgacgt ggatgccggg
     1801 agtaaagcca tcattaccgg gacacgtatt gaaacgaagg acatgacgga cggcgacgcc
     1861 gcgaccttcc gggagatcgt ggagctgcgt cccatcgccg ggatcaattg cacgccgccg
     1921 gagtacatcg agcggttctt ccgatggcgt ctggggctga aggaccgagt tccggcggag
     1981 ccatcaggag acgagggagc ggagaaggaa tcgacgcgcg gtgaggacgc cgccaaagac
     2041 agggacacgc ccgacacgcc ggacagggaa gccgcaaaaa aggagacaaa ggacactgcg
     2101 aaagatgata aaccggccgc gacgtcgatg gcgcgtctgg gcgtgaacgc gttcaccgcc
     2161 aacggcgtgg agcagaaggt cgcggccgat ctgaccaaca agctgtacgc gaagctgcgg
     2221 gcgtccaagg gcgtcgaggc ggtggtgatg cgcggcggcg gccgcacctc ggcgcgccaa
     2281 ctcatgggcc gcgtgagcaa gctcggcgcg accaccgtcc tcgccctgcg cgtggtggac
     2341 ggccagagcg gtaggctcgt atacagccgg agcctcaact acaaggatgg agaggacatc
     2401 gacggcaggc tcgacgagat cgccgggcag atcggcagga agggtgagat ctgggcgggg
     2461 gcgaagtagc ggatatcact gtaaatctac attcgggcaa ttgatagttg cattcatgaa
     2521 atttcattat gaccttgaca cccccctttc ccctaccgaa ggggaacggg gccgggggat
     2581 aggggtcaga gccgcgtttg atacgcaccc cgtagggact tcaaagcgag atcgatggcg
     2641 ctaacgacgg tcgcgtgcgg gtttccgtaa tagcatgagc gatcctgttg cggggtgggt
     2701 gcggttgcgt ttgtgaaacg caatctagcg gccgccgcgg acggggatga cgtaaaaaaa
     2761 atagtcagag taggttttgg gttgtgcttg aaatcttggg acataaattc caatatgata
     2821 cgaccgatca attggaatgg ccccataacc tgtcgatgtt atataatagt tttggacatt
     2881 agtgaaaccc tgattctgca gccaggttgc gatatggtat gaaaaattcc atagacttgt
     2941 aaactcgcgc acattgggaa gacgccagtc ggaaaagccc agatagtttt ctgtgttgag
     3001 cagtgcaata taattcagtg ccgttgtaaa tgccactcgt ccgtcattgg cggtaccatc
     3061 aacatcatat cccggattcc ggcttaccat gatattacca tcctttgccc acacaagcgc
     3121 cgtcaggttg tctgttattg tcccattgct attgtcggtg aaacgtggtt ccggccacgg
     3181 ctcccctttt ctcagcctgc cgtcatcatt agtgtaatag gttacggttt gtcccgtttt
     3241 ttgcaacact ctctccgaac ccgcattctc tccacgaacc ggccagatgt aataaaattc
     3301 cgttttaaat gacatccaag ccccaccggc agtgatcaaa tcaacaaaaa aggctgagtt
     3361 ataactaaga tcaggatttg tagtcgatga ccagatacgg gcatttaaag gaacattact
     3421 aaacccttca ctcataagcc aagtataaat atatgcctgc ccaaaatcga tgagactcat
     3481 aagttcgttg gcattgggag cgcgccagtc gctatagccg agatattttt ctccgttcag
     3541 cttcgatatg tagtccatgg cgtgatacca ggtaaccaag ccgtcttcgg ccgttccatc
     3601 gccatcaaat ttcgggtccc gcgtcttcat gatattcgca tcggccgtcc acatgagtcc
     3661 ggtcaggttg tcgcgcacgg tgccgtcgcc gaggtttatg aagcggggcg ttttccacga
     3721 atagcccttt ttgtagtacc cgtcatcgta gagcgaaacg ctgttctcct gccccgtccg
     3781 catcacataa aacaccggct gcccggccgc gctcgacagc ggcgacgaga aggcgtcgct
     3841 ctgcggatcg ttgggattgg tgcgctccat ttcgataaag caggccggtg ccagcggcat
     3901 gcagagaaag atcatgatga aatcctctgg cgcatggatt ccagctcatt tcttatattt
     3961 gttgacaatt aaccatacat ttacagcttt gtaaagaaat taatccgtcc gtattttttt
     4021 aaaaattgtt tactatttat ttcggtcgaa ctatttgagt gttaccaatt aaagttccct
     4081 gtcatcgcgc gaagcgatcg cgacgacagc gagataatca acacacgaac atttccctgc
     4141 aatgcctgtc gtcaatatcc agcgcccaca caaaggccgt atgcccagct cgctcacagc
     4201 atctctcacc gcacttacca tcctcctcgc cctctgctcc gccgccctcg cccagccgaa
     4261 ggatcccgag gcgccgcggg cgccggtcgt tgtcgatgag aaggcgggtg cgaaaaagga
     4321 acgcgcagcg aatcagcccg aaaagagggc cgacctgtac gcctacagcg tgaaggcgct
     4381 gcgcgccgac ggcaggtccg tcgaggggac cgcggacctc gcatcgccgt cgataacctt
     4441 tacccacgta aagggcgggg tttcatacga gaagaccgtg cggtgggacg atgtcgaaag
     4501 cattcgcgcc gtggagtgga agcccgtggg acacgacaaa acctccgcga aggaaacgca
     4561 acccgtgttt ttttacccga gccgctggaa gctcgtcctc aagtccggca agctctacga
     4621 cttcacgggg cagatcgcgc tcttcaataa aattgtcgtc aagaaccgcg acgggagcgc
     4681 ggcggtgtat acgtattttg tcgactactg gaaacgcacg ggaacgcgct cgggaatgtg
     4741 ggaaaacgcg cgctccaccg atttcgacca cccgcgccgc caccccgacc ggagtgcatg
     4801 cgtgcaggtg cgcttccgct agaacaatcc cgaggcacgc cggccgctcc gtccgttggg
     4861 ggatgattcc tatcggtaca gccggatacg aatttccgtc cgatcatcgt gagccccggc
     4921 ggcgattgat ccatgagcgc catcaggcca ttcgaaatcc cgcatgaatg atctttcccg
     4981 tgacagaaac cttccgaaac aagaaccaat accgaaaaaa tatttattga cataattttt
     5041 acatcgttta tcaatatgca aattgaagat attttcttca atttgcgcaa acaggctctt
     5101 atgattaaac gaagactggc acgcaaagca aaggagctat ttacacagta cccggtgata
     5161 accattaccg gcccacggca atccgggaaa accaccctgg ccaaaaacac cttcccccgg
     5221 ctgaagtatt tcaatctgga ggatatcgaa accagaacat ttgcccggga agaccccaag
     5281 ggctttttga agggctgcgc gggaggagcg atcctggacg agattcagaa tgcaccggac
     5341 cttttgtcct ccattcaggt atatgtcgat tcgctcaacc gggagggatt gttcgtattg
     5401 accggaagca ggcagctcga gctgatggaa tcggtttccc agtccctcgc gggaagaaca
     5461 gcgcttctaa aactcctgcc gttttccatc gaggaacttc acgaacacgg aaacatcttg
     5521 gaatccgatg agtatctgta tcatggattc tatccaagaa tacacgataa aaaactcaac
     5581 ccatctgaag cgctggctga ttattacaca acatacgttg aacgggacct gcgacagctt
     5641 gccaacatcc acaaccttca tctttttcac acattcgtaa aacactgcgc cggaagagtg
     5701 gggcagctga taaacctttc ctcgcttgcc agcgataccg gcataagcca tacaaccgcg
     5761 caggactggc tgacactcct ggaggccagc tacattatct tcaggcttca gccgtatttc
     5821 gccaatatcg gaaaacggct cattaaatcc ccaaagctct atttctacga cgtgggtctg
     5881 gcggcgcact tactgggcat tgaatcaata caacatgttg cgcaccatcc cctcaaggga
     5941 aacctctatg agaacatggc gatatcggaa atattaaaat accggtttaa caggaacaaa
     6001 accaataatc tatttttttt ccgggacgcc agaggccggg aggtcgactg cctctatacg
     6061 atctcacata aaatctatcc cattgaaata aagtcgtccc gcacgtttaa ccgggatttt
     6121 ttaacatcca ttgagtattt catggacgca attcacgaaa gcgaacgggg ctttcttgta
     6181 tacgacggag agggcgacat tgacattcgc aacataaccc tgctttctcc ccggagcata
     6241 tgggagaagc tggatgcgct gggttgatag cgcatttagg tgatcgccag cagtctattg
     6301 catgactcga tatcgtcacg cgcacggcaa acggcatgga tacacactct cggtaatact
     6361 ttcgggcacg tgtttcctgc gatgcgtgcg tgcaggtgcg cttccggtga ggccgctgaa
     6421 ttgaactgat agaaaaaaac ccggcaagaa aaacaatggc cgccagatgg agcgttgaaa
     6481 tgacgcccca tccctcggcg gatctagtcg ccttccttcc gccccccgtt gaggctcacg
     6541 cccgcaagcg acagcatgat gagcgcgccg ccggcgacga ggttcaccgt gagcgcgtcg
     6601 gcgaagacga ccgcgccgag gacgccggcg aagaggacgc ggctggccga cacgagcgcc
     6661 ccggggcccg cgtcgatgaa gcggtagccc tcggtgatga atacctgccc gacgagcgcg
     6721 aagagcgtgg tgagcgcgat gtggacgatc atcatcccct gcggcacgac gaagttcgga
     6781 agcgtcgcga ccaggcacac cgccgtgccg atggccatga gatgaaagag gatcacctcg
     6841 ctgtcgtcgt acttgcgcga ttcgcggagg gcgcacaccg cgacgcccgc caccacggcc
     6901 gagaggagcg agaagacgtc gcccgcgttg atcgccgcga agcccggcat catgatgagc
     6961 cagctccccg cgatcgtgag cgcgacgctc acccagccgc gccagctccc tcgttcgcga
     7021 ttgatgaacg gcgccatgag atacacgaac accgggtagg tcatgttgag catattggca
     7081 ttggtcacgg tggtaaactt gatcccgagg tagaagaaaa taaccgcgac ggcattggac
     7141 acgccgcgca gcgccacgta gcgcttctcc accgcgcgaa acgtcttttt gcgggcgagc
     7201 atgtacggca cgatcacggc gagccccgcc gcgaaccgga agaaggcgat ctcgatcccg
     7261 gagatggccg actgcgtggt gacgaacttc gcgaagaccg tcgagagcgc gaaggcgagc
     7321 gccgagagcg cgatcagcac gatgcctttg agggcgtgat cgttcattga tgatcacctt
     7381 cgatggcgat gccgcaaatc atcccgcaca aagcataccc ctcggaatcc gtcacggcac
     7441 gttcgcgcaa tgaccgtttt ttcacttgac acaagatagc acatatgcta tcttttaaac
     7501 atgttggtcg ttcttgacac caacgttctc tatcaggcgc tccggaaccg ttccggggca
     7561 tcgcacttca tcctttccct gatcagggaa aacaggctgt cgctcgcgat atccattccg
     7621 gtccaccagg aatatcagaa agtgttgtca cgcaccgctt cgcaaaaaga cctcaatctc
     7681 actcgaacgg acatcgatcg ggttttgcgt ttcatcgcct acatcggaag gccgtacgcc
     7741 acctattttc atttcaggcc caatctgcaa gatgaaagcg acaatatatt tgtcgagctg
     7801 gccctggcaa gcaattgcaa ttaccttata acgagcaaca caagagattt caccatcaag
     7861 gccgagttga aattcgacga ccttcgcatc atcaccccgt caaattttgc cgcaacctgg
     7921 agaaaagccc atgaaagcta aatcgagcgt gcttaccatt cgcgttcccc gggcgctgaa
     7981 aacccggatc gagcgcgtgg ccgacgaaca gggcgtttcc atcaaccagc tggcgctcta
     8041 cgcattcacc aaggagatca cggaaatgga atcggcatcg tatttcagct ccctccgcaa
     8101 atcaaaatcg aaaaagcagc tatacgatga tttcgattca gtaatgaaaa aagtcggctc
     8161 cgtcacgacg cccaaatggg acaagctgta atcttatagc acgtcacaca tcacccatgc
     8221 ggctgatccc ccgtatcaac cgcatgccgt tattgtaaaa cacggcgtcg taaaattcct
     8281 gcgaaagccc gagtcccagc agatgctcga tctcgtcctc gcgcggcatg atgaggttcg
     8341 ggaagtccga tccgtacagg atgcggtcgc ggtatttctc gaggagtgcc gcgtccagat
     8401 cgcaatagcc gtcgaattgc ggcaaaaaga cgaaggccgt gtcgagatac atgttcggga
     8461 aggcgtcgag gaaatcgacg aacgcggcgt actccagcgc gcccatgtgc gcgaccgtga
     8521 ccggcagttc cggatagcgg cgcatgagcc tctcgaaatg cgcggcgccg acgaactcgt
     8581 ttcctaccgg accggtgccc gtatgcagca gcacgcgctt gccgcgctcc atgatgagtt
     8641 cgtataacgg gaagaggcgc tcgtcgtgcg gatagaaacg ctgcactaaa agctgcagct
     8701 tgatccccag caccttcggg tgcgcaagcg cctcgcgcgc atagtcgaac gcgtcgtcat
     8761 cgccgggatg ataggccgcc aggcagtaca ggtccggcag ctcatcgacc acggcgcggt
     8821 tccacgcatt gagcccgcgc gcgatccccg gccggtgcgc gtagttggag tacacgatgg
     8881 ggcccacgcc gcgctcgcgc agataggcga cgcactcgcg ataatacagc tgatggatca
     8941 ccgaccagcc gtagtcggtc acaaaatgcc tccagatgga ttcgaagagc cggtccggga
     9001 acaggtgcac gtggaaatcg acgatgcgcg acggaagcga tggcatggct aacggcgccc
     9061 cccggggacg gaccctgccc gacgatcgta cgggacgacg cgcacgtcat tggaaactcg
     9121 ctcccgaacg gcctgcggcg aatcgatgcg gcgtcttgcg tttttcatgc cgatacgttt
     9181 ttcctccgac ggcccctgtc aagagggatt tccatcttga cgaatatgcg ttcatagcgc
     9241 aggatgatgg cgcgacattc cccggaacgg tgataccatg aagaaaaagc ccgacgccgc
     9301 gcccgcgccg aaaaacccca tcggcgcgaa actcgccgcc cagcggcgaa aaaagaaact
     9361 ctcgctcgag gtgctcgccg agaagaccgg cctcaaggtg cgcgacctcg aggcgatcga
     9421 gtccggcgtc gtctttccgc cggtgggcga catcctcaag atttcgcgcg cgctcacggt
     9481 ggacccgggc caactgctcg gttcgcccgc gaaggacccg gacgagcgca aaaagaggcg
     9541 catcgacgat ttcaacaagc gcgcatcgtc gtacctctac accatcctca ccccgcaggc
     9601 gatgaacaag cacctgcgcg ccttccgcgt ggtcatcccg ccgggcgcgg agcacccgaa
     9661 gatcagctac cagcacgagg gcgaggagtt catctacgtc ctcgagggga cgctggagat
     9721 caccgtgggc cgcaaaaagc acgcgctcaa gcagcacgac gcgctgcatt tcgactctgg
     9781 gatcacgcac gcgctcaaaa atccgggcgc aaaggaatgc gtcctcgtcg tcacggtgta
     9841 cacgccatga accttcccct cacgcaggaa cagataatga tccgcgacat gatccgcgat
     9901 ttcgcgcgca gcgagatcga gccggtcgcg caggactata accgccgcgg cgagtacccg
     9961 gcgtcgctcg tcaagaaact cggcgagctc ggcctcttcg gcatgatggt gccggagagc
    10021 tacggcggct cggcggccgg cgcggtgagc tacagcctgg ccctccagga gatcgccttc
    10081 tcgtgcgcct ccatcgccgt cacgctcagc gtgaccaatc tcgtggccga gcccatgctc
    10141 gccttcgcga ccgaggagca gaagcgcgaa ttcctcttcc cgctcgcctc gggcgagcac
    10201 atcggcgcct tcgcgctcac cgaacccgag gccggctccg accccggcgg gctgtcgacg
    10261 tccgccgtca aaaagggcgg cgtctatgtc ctcaacggcg tgaagcagtt catcaccaac
    10321 ggggagtacg ccggcgtttt cgtggtgatc gcgcgcaccg cgcccggcaa gaacggcctc
    10381 tcggcctttc tggtgccgcg cggcgcgaag gggctgtcgg tgggcaagga ggaggacaag
    10441 atgggcctgc gcgcctcgaa caccgtggag atcgtgctgg acgactgcgc ggttcccgag
    10501 gcgaacatgc tgggcaagcc cggcatgggc ctgcagattg cgctcaacgc gctcgacagc
    10561 gggcgcatcg gcatcgcctc gcaggcgacg gggatgatcg ccgcctgcct ccacgaggcc
    10621 gtgtcctacg ccggcgagcg ccgccagttc ggcaagccca tcatcaagca ccagaccatc
    10681 cagaacatga tcgccgacat cgccgtggag cacgaggcgg cggcgctgct ggtgtggagc
    10741 gcggcgtcgc tcaaggacgc gaagaggccc ttcacgcgcg aggcctccat cgcgaagctc
    10801 gccgcgaccg aatcgctcaa ccgcgccgcc tatcgcgccc tgcaggtctt cggcgggtac
    10861 ggctacatcc gcgacaacaa gatcgagcgc atctaccgcg acgcgcgcgt gaccacgatt
    10921 tacgagggga cctcggagat acagcggatg atcatcgcgc gggagacgga ggcgctgctt
    10981 tcgcgctagc ggacggcggg aacgggcgcg gcgccggcac cggagacgac tccccggatg
    11041 ccggccgacc ggaacgaacg cgtacggctc agcgcatcgc gtctttcgag aaccgcgcgt
    11101 cgcccaggtt catgttgtat ttcaccgact tcacgttcag ggtggtcttc ccgctgccgt
    11161 cggacatggt catgacggcc ttcatggggg tgatgatgcc ctgcaccgtc ttgatctcgt
    11221 agttttcaag atacttgtgc agccccccct tcatgtagac gtcgatgcgc accacgaaga
    11281 agtccgattt gcgcacatac agcaccgact tgctgtacac ccgctccccc ttcttcttga
    11341 ccgattcgac cacgtagcag tcggcgcccg ccgcctgctt gtcgccgaga tacttgtact
    11401 cgtaatcgtc gatgttccgc gagctcaggt cctcgtaata caggtgggag ttcacgaacg
    11461 acttgtcctt ctcggacgag gagatccgct tcaccttgcc ggatgagagg cggagccact
    11521 gatcgtcctc cgcgcccttc ttcgagtgcg tcaggaactt gatggccgag ggtcgcgtga
    11581 aggtgatgag ggccatgtcg cgcgggcccg ccttcttgct gatgagctcg aattccttgt
    11641 ccacggtgct gccgcccttc tcgatcacca tcacgaccgt gctggtggag gtattgggct
    11701 tcgtgagcgc gtcgctcttc tccatgattt gcctaccggt gagggcgaag gccgattgcg
    11761 ctgcgcacag tacgattatt acggcgccga tcatgcattt tttcattaaa tcctccaaaa
    11821 attaaaactg gctattgaca aagtgataaa acgatagtat ctataatgaa cgaacgtttg
    11881 ttttttatgc aagccatatt tttacgattt ttacaaattt cgacaatgct cgcgatgaga
    11941 aaaaagaccg caccgacgac agaacaggcc gccgctccga acaaggagcg gatcgtcagc
    12001 tcggcgaaat ccctgtttgc cgccaagggc ttcgcggcga cgggcctgcg ggagatcgcc
    12061 gcgaacgccg gggtttcgct cgggaacatc tacaaccact tccagagcaa ggaggagata
    12121 ttccagagcg tcttcgattc gaagcagatc gaggaatcgc tggccgacac cttcgtgctc
    12181 atcgcggacg acttcccgct caacatcgac aaggtgatcc tttccatcaa gcgcacggtg
    12241 gacgcgaact acgagctata ccggatcgtc tacatcgacc tcatcgagtt cggcgccgtg
    12301 aacaccaacc ggctcctgga gaacctgctc tccttcgggc agtccatctt ccgggaaatg
    12361 ctgtacgata aggcacggcg cggcatcctc aaggagctcg actacgactt cttcgcgcgg
    12421 cagttcctca tcgcgcaaat atcgttcttc tcaagcctgc gggtgctccc cgccatacgc
    12481 atggaccgct acaccgacga agagatatcc aaaatgatgg ccaatgtgat cttaaacggc
    12541 attcggaggg agcctaatgc gtcgtgagac ccacatgcag gggctgatcc gcagcgcctg
    12601 cgcgctcgcg ctcgtcgcgg ggctggccct cgtcattagc gtcatcatcg tccccgaatg
    12661 gcgggccgat tcgcgcgcat gcgcgtcccc cattcgcgtc gccgacgacg ccgccgcggc
    12721 gagcgaatcg ccgaaggata ccgcgccgac caaaccgacg aatgatccgg aaaccgcgcc
    12781 ggccaaggac ggcgcggatc ataccgcacc gaaaacgtcc gctccggacg atggcgcggt
    12841 gaaagacgat cgcggcgcat cgggcgacaa acagccggaa accacggacg acgccgaagc
    12901 ggcgggagac gaaaaaaaaa agccatgttt acgatgaccg ggttcgtcga gctggaaaac
    12961 ttcatcggca tccacaagaa cacggagtcg gacgagccct ataagaagtg cgagctgcgc
    13021 aaccgcacgc agctgcgcta cgggaccgat accgcctacc tgctcgccac cgcgaacctc
    13081 tacgccatgg cgacgttcct gagcgaccgg ctcggcaggg aataccgcta cagcgagaag
    13141 gacacggtgc accgcaacct gcgcatatcg tccaagtatt acgaggcgag cttcaacgag
    13201 ctctacgcga gctacgacac cgatcgcgtc cgtctgcgcg ccggcaacca gatctacggg
    13261 tgggggaccg ccgacgtata caatcccacc tcctatttca atcccttcga catgcgcgag
    13321 tatttcttca aggacgagga cgaacagcgg ctgggggtgc cgtcggtgtc cgcgatgttc
    13381 ttcatcggcg acttcacgct ggagacggta ctcatgccgg tgcacgtccc catggcgctt
    13441 gcgccgtcgg gtaatttctg ggcgatcgag tcgaagctcg gccggcagcc ggtgatcctc
    13501 gaggagggcg cggcgctgga cgcgaaaccg gagaacatgg gcgcgggcgc gcgcttcgcg
    13561 gggagcctga tgggcgtgga cgtgtcgctg agcgcctatc acggcccgta caaggagccg
    13621 ctgttggtgc ccgacggcac acgcatcctc ccgaacaagc cgctcgctct cgcggtgaag
    13681 cagcagtacc acgtcctcac catgatcgga acggacgtct cgttcaagat ggacaagttc
    13741 gtgctgcagg cggaggcggc gtactccccg gacaagacgg ggatggtgat gcaggacacg
    13801 gacgacatca ggcgcatcgc ctttccgttc gagacgcgga aatcgccgta tgtctcctac
    13861 tccgcgggct tcaactactt cattccgctt ggccgtctca tcgagggcca cgagggcgat
    13921 acggtgttca ccgccgagtg gcaccagtcg ctgtatctgg accgggagct cgcggacccg
    13981 ctgttcacca agatcgtatc gtgccggctg gaggacagct acttcggcgg acggctcaag
    14041 gtgtcgctca cggcgatgtt cgacacggcg aaatccggct acctgctgtg gccgcagctc
    14101 acctgggact tccagaacgg gctgtcgttc agcgtcacct acgcggccat cgagcacacg
    14161 ggagcatccg ccgggcggat caacgagaac gaatcggcgt tttactatct gcgcgacaac
    14221 gacatggtga cctggagggt gcgctatgcg tataaataac ggcgttctca tgatcctcgt
    14281 ggcggccagc ctcatcaccg cctgcgtgaa caagcaggag aagggcccca tcaccatctc
    14341 gggcaaaatc gacgcgagcg cggtgagcgg cagcgcgtcc ggccccactt tggtggcggt
    14401 cgcgaagacc gacagcatcg aggcgatcga gcgcgatccg gccggcgcca tcgtcatgat
    14461 cgccgggatc gacgagcaca gcggcaccta cgagctcgag ctgggcgagt acgacgtgca
    14521 gccgggcgat cccgtgacca tcatcgcctt catcgacaat gcctacgcga acggcatccc
    14581 gtatccgaaa cgcggcgact tcatcggctt ctacatcgag ccctcgtcgc tctcgacgag
    14641 ctatgcgctc aaggcgggcg cgaacccgaa cctcgacatc gccataaacc gcgaggtctt
    14701 ctcgttcgac gctgccgtct cggggaccgt gggcggaacc gacgccgggg acctcatcat
    14761 catcgcctat gcgggggaga tcacctcgtc ggacatgagc gcgctggaca cgagcgcggt
    14821 gatcggctac gcccgcgcca gtaagggggc ggcccccacg ccgtaccgcg tgcggatcat
    14881 gccctacggc tacaatgtgc ccatccagaa cgtgtacctc atcgccgtgc tcgaccggaa
    14941 ccggaacggc gtcgtggacg gcggcgacgc gatcggcttc cacgcggccg gagcgaacgg
    15001 cctcccgacg cccatcaccg tgaacgaggg cgagaacgcc gggatcgaca tcgccttctc
    15061 catgacggtg cccgttccct ccggcgtggc catgtcgatc aaggggtcct tcgccgtgcc
    15121 cgcgggctac aatgcctcgt cgaagccgat gtatctcatc gtggcgcgga tcgacaatcc
    15181 caagacgctg ttcaccgacc cccttgcggc gatcaagtac ttctacaaga tgccgccggg
    15241 cgagttcgtc ttcaacatcg acctctccaa gaccgacctc gtccccggcg acagggtggt
    15301 ggtggccacg ctctgggacc gggataatgc cggagggttc ccctatccga cgcggggcga
    15361 caagctgggg ttggcgcaga acaacgacac ctactcgtac ttcatcgagc tcgcgctggg
    15421 cgtgaacccg gtgccccagg agggctacga gttcaacacc aacaagctca tttacgattt
    15481 cgattcgagc gtgaagttcg cgctcgaccg gggcgccatc gagagctaca acgtcgacac
    15541 ggcgcagatc atcatcctat gcgtgcacgt gaacggcgtc gacatcggca tcaagccgtc
    15601 gggcgtgtcc gtgaagatcg acatggacta cgtgctcggc tccaccgtga tccggccgcc
    15661 ggccttcgat cacataacgt cggcgccgca cccgattctg cccgcggagg acatgcgcac
    15721 gctcgggatc ttccgggcgc tgtatcagga gatcgtcgtg tgggagaaca gcgtcccgcc
    15781 cgacccgctg atccagggcg tcgcaaaccc gagctccggc gagaccgaga cggcgtacct
    15841 gttcgccgtc ctggacagga acggtaacgg gaacctggac tcggacgacg ttgtgggcta
    15901 ctacaatcgg gagtacgtgg tcaccacggg cagcatcgtc atccccggtt acgggtcggt
    15961 ggcggtcccc aacgggacct actatcttcc cgcgaccatt cgcaccatca gcacgggcgc
    16021 gaacacgaac agcgacgggc aaccgtactg gatcacgttc cggtattacg atccgccctg
    16081 acgggcgcgt gacggcatcg cacacatttg aatcgaacga caccgcagaa gaggacacat
    16141 gaacaagcta ttcgacgtga tcatccggaa gtacaagtac atcatggttg cgatcgtcct
    16201 gctctcgctg cccatgggat actatttcac gcagtacaag ttcttcaacc acatcgacat
    16261 cttcttcgat gcgagcgatc ccaatctgca gttttataaa aacttccaga agcgctacgg
    16321 caacgaggag ctcggcgtga tcctctttaa ggcgcccgac ggcacggtct tcaacgagcg
    16381 cgtgatcacg gtcatccgga gcatatcggc gcagctcaag gaggccgacg gcgtgcagcg
    16441 cgtcttcagc ctcaccgagg ccaaggaggc gcaggcggag ggcaacacga tccgcttcgt
    16501 gaacatcatc ccgaagggcg agatcgcgcc gaccgcatac gacccgctca agcacaaggt
    16561 cctgaaaaat ccggtggtcg tcaacagcct cgtctcgaag gacggcgcga ccacggcgat
    16621 cctcttcgag ctgaagccca cgagcgacaa cgagaagaag cgcgagctgc tgcaacggat
    16681 catggcgttt tccctgaagg cggcgggcga cgcggtgcgg ctgcatttcg ccggcgtgcc
    16741 ctacgtcgag gtggagatga acatcctgtc gaagcgcgac tttctggtgt tcacgcccat
    16801 caccttcttc ctcatcttca tcgtcgtggc cttcatgctc aagcgcatct cgctcgcgct
    16861 gttgtgccag atgaacctcc tgctcaccct ggtctgggcc atcggcttct tctcgatgat
    16921 gggcgagtcc ttcaacatgg tcacggtcat catgggggcg atcctcctgg cgatcgcggt
    16981 ggccgactcc atacacatcc tggcgcacta ccgcgaggcc gacgcctacc acgacaatca
    17041 cgacgacgcc atccggcaca ccctgaagag catctggatg ccctgcctgc tgacgagcct
    17101 cacgaccgcg gtcgtgaggc tcgtcagcag gcagg
//
LOCUS       DAORHF010000051        16826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2507_length_16826_cov_5.228728, whole genome shotgun sequence.
ACCESSION   DAORHF010000051 DAORHF010000000
VERSION     DAORHF010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16826
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_16826_cov_5.228728"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /gene="thiD"
                     /locus_tag="PKJ16_06355"
     CDS             complement(<1..592)
                     /gene="thiD"
                     /locus_tag="PKJ16_06355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HNV46646.1"
                     /translation="MILPRVLTIAGSDSGGGAGIQADLKTVTVLGGYGMSVITALTAQ
                     NTQGVHGIMDVPLDFIERQFDAVLSDIGADAAKTGMLATTGVITLVARKLREYRIDRL
                     VVDPVMVAKGGASLLATDAVRTLIDELLPLAMVVTPNVPEAEALSGMRIGGIDDCRAA
                     AMAIHTRGARAVLVKGGHADGPNAVDVLFDGSGITEF"
     gene            complement(589..1647)
                     /locus_tag="PKJ16_06360"
     CDS             complement(589..1647)
                     /locus_tag="PKJ16_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46647.1"
                     /translation="MMKRRCGIILFCILAAAQSVSAQTPPVPHDVIWDEDIVETGTRG
                     IITAAGSIFSDLFTPMTHYRPGEWTVTLAPAYFQVSRISLEPVMKGKNLEGYGGGLGG
                     GYAFNEHVMVYALAGGMKTAGNIAGRSDKLLLYKDFDAKVKFDIFAAYAGAGFDVFGG
                     SKNWSMPIYVGAFTQKYDAELKFRPLNTKILDSIIGAFSYSSTIGVDVSGEDWLYGVS
                     AGIALSRNFFDTVRITPYYIYSKGLRATSLHARAYEDIRVQGSVLIPFDRTTSFSHDH
                     DVDIRSTDASVAGLCVSIISQKSWTFSLGIGTSLAAQVDAYSKSLGGLRMLSIALSVT
                     YNGGRAPDSSAGDGAGAR"
     gene            1800..2786
                     /locus_tag="PKJ16_06365"
     CDS             1800..2786
                     /locus_tag="PKJ16_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46648.1"
                     /translation="MTIRTITATILALVAAFCAACAQPRTAVTAAPEDPIHLLRNHSI
                     NPKSPLTFRVKMAPAPVLDYLRKLDQAPEYQNYLVRKSDMGQIATYLNLMPSKMRAVL
                     QERLIGIYFINNFKGSGLTEWVRDERNNFYAFMVFNPDTLARDLSSWLTYRERSCFIA
                     NDPDISIEVDCGKKHTGFLYVLLHEAAHVVDYAQGITPYVEDSLKSISPARGARTPFV
                     EGAWNGIRTPASPFDFPLRASLTFYGFGGGPKIPIARAIDAYRMLEKTPFVSLYGATS
                     WAEDLADYAFFMHLTGALKQPYAITVRKKGSDPYSIRPAETARKTLNRPYPL"
     gene            2937..3380
                     /gene="umuD"
                     /locus_tag="PKJ16_06370"
     CDS             2937..3380
                     /gene="umuD"
                     /locus_tag="PKJ16_06370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014841806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HNV46649.1"
                     /translation="MNDRARSAISEIYACAGTAGPALPFLLASIAAGFPSPADDYIDR
                     MLDLNEFLVRHPAATFFVRVAGESMIDAGIHPGDILIVDRSIEPADDRIVIAALNGEL
                     TVKRIRMESGRICLAPENPRFPAIEITPDMQFEVWGVVCHVIHRV"
     gene            3391..4665
                     /locus_tag="PKJ16_06375"
     CDS             3391..4665
                     /locus_tag="PKJ16_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015861548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HNV46650.1"
                     /translation="MKTIFALVDCNNFYVSCERVFDPSLAGRPVVVLSNNDGNVVARS
                     NEAKALGVPFAAPAHECAPLVKRHNMAVFSSNYALYGDMSQRVMSIVAGFAPEMEIYS
                     IDEAFLSLGDLAGVDPLDYAARIRETVRRWTGIPVSVGIAPTKTLAKLANRLAKRDPA
                     CGGVLALDGRSDLDSILDGVATGDVWGVGPRYSAMLARHGIRTARELRDASDAFVRKR
                     MTVTGLRTVHELRGRSCIELEGAAPPKKGIVSSRSFGRPVVELAELSEAVASYTARAA
                     EKLRCQRSAASLMCVFVSTNPFRDDPQYYNIITRRLPAPTADTAELIRHARADLERIY
                     RPGFRYKKTGVFLSEIVPADSLQLDLFAAAEPSEERARLMATVDIINARLGRGAVYYA
                     AEGVRKAWRMRQERLSRRFTTRWDELPVAVAG"
     gene            4696..5163
                     /locus_tag="PKJ16_06380"
     CDS             4696..5163
                     /locus_tag="PKJ16_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46651.1"
                     /translation="MMGIETTVCMSLANIERLGWASRVTGLSLAHIVRALLERMMLDK
                     RVRTGLWGRVRYQARGADNNMCRLNVVFREDVYEYNLDMRKAYKMSVSLILAIAIERY
                     LDSVVRELSGTGGSTDNYRFNSYVISKDIVDGVICWKSYWGFPTKIPELIHLE"
     gene            complement(5183..6007)
                     /locus_tag="PKJ16_06385"
     CDS             complement(5183..6007)
                     /locus_tag="PKJ16_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017713738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV46652.1"
                     /translation="MKKDTVVLISGCSSGIGLALAREFDRRGYYVFATARRASTVDIR
                     SSRADISSLDVTNPKSIEMCVKHVMRQAGKIDILINNAGYALMGPVVDLSMDDFRRQF
                     DTNVTGLVGLTQAVAPHMIKQHSGTIVNISSVSGFLTQPFSGAYCASKAAVSSLSDAL
                     RMELAPFGVRVVTVQPGSIKSQFGETAAKGLDRYERSVYAPVKEFIEARAMTSQQNPT
                     TAEEFSKELITRLEGKKVPPVIRLGNDSLRLFLESKLPLKTLDRMLSKRFGLDRLK"
     gene            6175..6726
                     /locus_tag="PKJ16_06390"
     CDS             6175..6726
                     /locus_tag="PKJ16_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV46653.1"
                     /translation="MLTDPIPSRAECYRLIDEHRMLPNIREHSERVMLVALAIADNLR
                     AGMRVNRDLVVAAALLHDIAKTRSLSTKEKHDITGADLLRDLGYARVAEIVRAHVFFS
                     NFIEDGPLEDREIVYYADKRVMHATVVSVHERVEDLVARYGKTPEIIERITANKRLIL
                     AIEGKIQRAMAIAIDEAIANIDE"
     gene            6723..8369
                     /locus_tag="PKJ16_06395"
     CDS             6723..8369
                     /locus_tag="PKJ16_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46654.1"
                     /translation="MKPRYCILDCYVDEPACFGVPPFLAPYARYIYGALVDGGAAPES
                     IRYATIDTLRASGYRLHDRFDHVFMIGGAVVPGRYLGARIGTTDEMAAIARENPETPF
                     ALGGLAARFMDANAQRNLTVVAGDIELFAHGTARGEVRRGLRTTDEIARWARTGANVV
                     RQHPHFPDIICEIETYRGCPRQAHCSFCSESVHGAVSFRDGGDILAEIERLIDAGASR
                     FRLGRQADILLYGSPLRDFANGFPRPDPVPVIELFEMLRTMRDDGRIALLAVDNANPG
                     TIANFPEESGRILSAIAASITPGDTLALGVESLDPAIIARNTLKVDAAALLEVVRLVN
                     DICGHRVDGIPVLLPGINLIHGLIGETPETFRINFEGLCEIRDRGLLLRRTNIRTLLP
                     FPGTPAAARRGAESTSVRNRYEHYRDRIRREIDHAMLQRVYPAGTVIRDARVEESRDG
                     YSYARQIASYPITAKVPLALEPGAAADLVVVGHQERSLVALPLPVRINALPATALSHL
                     PGIGKRRASEIVLRRPFTRPEEAADLIADVPPALRARIGV"
     gene            complement(8379..8705)
                     /locus_tag="PKJ16_06400"
     CDS             complement(8379..8705)
                     /locus_tag="PKJ16_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46655.1"
                     /translation="MSDELKSVLMKILAVVLYLGLGALVSWIFYYLKKRDLFGGYIGG
                     LVVGVIGALIGGLVLDRLLLDIAIKVLKFLAIDAGVNVFAGFIGAYAAIYIMNRLNHN
                     KERKKY"
     gene            complement(8766..9374)
                     /locus_tag="PKJ16_06405"
     CDS             complement(8766..9374)
                     /locus_tag="PKJ16_06405"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV46656.1"
                     /translation="MIFVINEENPQKRFIDKARAVLEEGGVVVYPTDTVYAYGCDIRD
                     RHAIERIYRIKHIPKNKPLSFIFSDISGLHEYVRNISDEAFKIMKKAFPGPYTFIFKA
                     SKLVPKIVITNQKTIGVRVPDSRIALALVQALGRPILSASVSASSGEYIVQPEDIEKQ
                     YRHEIDLVLSCGPMVSTPSTVVDFSEGALRVVREGKGELFFI"
     gene            9612..11639
                     /locus_tag="PKJ16_06410"
     CDS             9612..11639
                     /locus_tag="PKJ16_06410"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HNV46657.1"
                     /translation="MSEIELKIKSRDIGTLIATIKANNPAAGLDLVMKAYELAEAAHR
                     GQVRLSGEPFIIHPLEVAITLAALKLDTDTIVAALLHDTVEDTTITLEKLKRDFNENI
                     ALLVDGVTKISSLKKKSKHTEQAQNLRKMLMATVKDVRVILIKLADKLHNMRTIMFQP
                     PAKQRQVAEEVLDIYAPLAGRLGISAVRSELEDLAFHVLNHDDYHEIKEMVAHRKHEI
                     EEFISGIQTVLKQKLAELDIDADITGRAKHYYSIYRKIKLQKKSFDEIFDIRAIRIIT
                     DKIKDCYSVLGIIHTLWTPITARFKDYIAVPKSNLYQSLHTTVMGPGGHPLEVQIRTS
                     EMHAVAEMGIAAHWIYKESGRKKDYRNPTILKNINMWESEEKNTREFMKELKMDLYEE
                     EIFVFTPQGKIITLATGATPVDFAYAIHTEVGNTTSGARVNNVLVPLKSELKSGDIVE
                     ILTSKKAHPSEAWLKFVKSSNARYKIRNWLRQQSESQKKAEHAAHDEHRRKKTAEVSI
                     PEPELIKVKKLSGHSKAVLSVDGTSNVMIRLSQCCQPIPGDDVIGFITRGRGITIHRK
                     TCPSLARLRLEKERFISIVWEDSETTYPVKLSVTAFDRPNLLKDLAEEIGLAKANILK
                     AEAQVIEKGSARFKCTLEVKSLAHLQDIIARIKKIKNVTSVYKVNEKVVIK"
     gene            11636..12310
                     /locus_tag="PKJ16_06415"
     CDS             11636..12310
                     /locus_tag="PKJ16_06415"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HNV46658.1"
                     /translation="MTRSTQRDRDSRPLRIVLGSSSPRRREILGALLDGRIEIRHPEA
                     DERVAPGEPPADYARRVAHAKAQSVCSSLGPPDGSDVLVIASDTIVTIDGRIIGKPND
                     RADAERILASLVGRTHQVISGLTLAHRPALGAPDSWNMHTGLEATEVTFFPLSPDGIA
                     RYLDCIDYADKAGAYALQENGGMIVRSVNGSMTNVIGFPLRLFFGMISALTGADAVFI
                     SSSRAP"
     gene            complement(12285..12824)
                     /locus_tag="PKJ16_06420"
     CDS             complement(12285..12824)
                     /locus_tag="PKJ16_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46659.1"
                     /translation="MKQRKQYVIDRKFQLKHTFSVLGVVLVLVAVIIGIIGVNAAYNN
                     NRLDNIMGIQDNIVDAMMAWVQNPAEKPQQTAIKAIAGKHFENLTTIKRIIRYNTILI
                     GLIVVIVLLQSVIMYFVMIRMTHKISGPIFVMSKYFADIIDGKMPNPRKLRQGDELQD
                     FYNLFTKMVEALKAREKKK"
     gene            complement(12894..13607)
                     /locus_tag="PKJ16_06425"
     CDS             complement(12894..13607)
                     /locus_tag="PKJ16_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46660.1"
                     /translation="MKKTRLVVAVSIAGLCVLGMTVLFAQTEFKHGFMATEPFTKARM
                     QFLPVPGDFRNYFVVQSIDDNTNILIGDFVGSDKMFSLVMDDGSDGKIDRVIEYFPDS
                     KKFVNPARPTTKFFTNLNDFKREVIEGSIFRNNYTYKMSSLDLLKSRIEKGRDIFRAD
                     YNYNVKIYDPDRPSTVMSEFFFGKKLGRYDLIFATYYYKIFYTQISPVVSYSVYCRNS
                     KDPVVAEIVESLIKMVPKS"
     gene            13735..13806
                     /locus_tag="PKJ16_06430"
     tRNA            13735..13806
                     /locus_tag="PKJ16_06430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13767..13769,aa:Gly,seq:ccc)
     gene            complement(13879..16299)
                     /locus_tag="PKJ16_06435"
     CDS             complement(13879..16299)
                     /locus_tag="PKJ16_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46661.1"
                     /translation="MNSLLALLVDFCYRYAFVRHLIWGMMAALLTALGVAAFYAFGPV
                     ESAPVGWERSVLVSPPALNARNAAAVAKGNYLAAVYEALEGDRKTLYVSISFNGGRSY
                     IAPVRIAEVLSKVDANPHVAISGAGHVAVIWQNLQAEDSKTRLFISQSKDMGATWSEP
                     RRIFTQYAAPIEMEMLAQVIYDELGALHLFYHAFLGGRLTFFHSKSPDENLFDVGDAL
                     VDVDKGLRGAFLPSINVSGSTIYIVWQGKGEKLGVLSDDLFLIKSSNHGRSWTGSVRI
                     TESTANEAAPSLAVYKGILYLAYQTNETKNWSIAMVRGFNDGEEWERSPVRISDTNAN
                     CYSPAIVATRNDEIVVAWHDTRQANTGIYGRRYSIEDRKLSPELRLSGAASVARRPAA
                     ASVGPMVVLFWEEGARIIAKYSDVSAEPPVVFSGTHPEGVWSKASTAVINWRPPADES
                     GILGYATIVNRLPVFNPTIRNLDANFQSMTLSDLEDGVNYFHIRTVDGAGNFSRTIHF
                     RLQVSRNPPAAPVVVSPTHPEGTPVPSPSPTFQWSTDEGERLKGFVFSFSNDPLKAPD
                     QFTTNLSMNFSNLANGRYFFKVRSVDATNTFSRTAAYEIIVGEAAPLDPSMYERMARD
                     GSATKEPTPGRVPGVIVPHVAVTLPFDAKRPLARAEFDALVSPRNIAAGSVLGYAYAV
                     TNTRTPPPDAVNLRGAILRVRDLSDGEYYLAVKARYSAVIDGRAETRWTAPAYVRFTV
                     QTPPSDSPVLAALDDTLRRIQARFAAVFMYTVTALVTVITLGFGSRLAFYARLLRFRL
                     VSAVRGMW"
     gene            complement(16310..16669)
                     /locus_tag="PKJ16_06440"
     CDS             complement(16310..16669)
                     /locus_tag="PKJ16_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46662.1"
                     /translation="MSSIDGGKARARVKKLFFRKLLPLSDMARRGAAYFPMGADPSAA
                     TYYVARNATRMSRGDFEAPVCADENEFGKKLAALWERSGNPELAKCAPEMAACAGLLY
                     APEEIDDEVSPFIYVMF"
     gene            complement(16656..>16826)
                     /locus_tag="PKJ16_06445"
     CDS             complement(16656..>16826)
                     /locus_tag="PKJ16_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV46663.1"
                     /translation="SFNVRGEPIVCSPADAYRCFMRTEMDLLVLEDCLLWKSNQPAFA
                     EAGDWRKDYELD"
BASE COUNT         3277 a         5451 c         5044 g         3054 t
ORIGIN      
        1 caaactcggt gatgccggac ccatcgaaga gcacgtcaac tgcgttgggg ccatccgcat
       61 gtccgccttt gacgagtacg gcgcgcgccc cgcgcgtgtg gatcgccatc gccgccgcac
      121 ggcagtcgtc aatgcccccg atacgcatgc cggagagcgc ctccgcctcg ggcacgttgg
      181 gcgtgaccac catcgcaagc ggcaggagct cgtcgatgag cgtccgcacc gcgtccgtcg
      241 ccagaagcga cgccccgccc ttcgcgacca tgaccggatc gaccacgagg cggtcgatcc
      301 ggtattcgcg aagctttctc gcgacaaggg tgatgacgcc cgtcgtggca agcatcccgg
      361 tcttcgccgc gtccgcgccg atgtcggaga gcaccgcgtc gaactgcctc tcgatgaagt
      421 cgagcggcac gtccatgatg ccgtggacgc cctgcgtgtt ctgcgccgtg agcgcggtga
      481 tcacgctcat gccgtacccg cccagcacgg tgacggtctt caggtcggcc tggatgccgg
      541 ccccgccgcc cgaatcggaa ccggcgatgg tgagaacgcg ggggagtatc atcgggctcc
      601 cgccccatcg cccgcggacg agtcgggagc gcgaccgccg ttatacgtaa cggacaatgc
      661 gatcgagagc atgcgcaacc cgccgaggct cttgctgtag gcgtccacct gtgcggcaag
      721 ggaggtcccg atgcccagcg agaaggtcca gcttttttgc gagatgatgg acacgcacag
      781 ccccgcgacg ctcgcgtcgg tgcttcgaat gtccacgtcg tgatcatggg aaaacgaggt
      841 tgtccggtcg aaggggatca ggacggagcc ctgtacgcgg atgtcctcgt acgcccgcgc
      901 gtgcaatgac gttgcgcgca gccccttgct gtagatatag tacggcgtta tgcgcaccgt
      961 atcgaagaag ttccgcgaca gcgcaatgcc cgcggagacg ccgtagagcc agtcctcgcc
     1021 gctcacatcg acaccgatgg tggagctata ggaaaacgcc ccaataatag aatctaaaat
     1081 tttagtattg agcggccgga atttcagttc ggcgtcgtat ttttgggtaa aggcgcctac
     1141 atagataggc atggaccagt ttttgctccc gccgaatacg tcgaacccgg cgcccgcata
     1201 cgcggcgaag atatcgaact tcaccttcgc atcgaaatcc ttgtacagga gaagcttgtc
     1261 gctgcgcccg gcgatgttcc cggccgtttt catgcccccc gccagcgcat acaccatgac
     1321 atgctcgttg aacgcatacc cgccgcccag tcctccccca tagccctcga ggtttttccc
     1381 cttcatgacg ggctccaggc tgatgcgcga cacctggaaa taggccggcg cgagggtcac
     1441 cgtccactcg ccggggcggt aatgcgtcat gggcgtgaaa agatcggaaa agatggaacc
     1501 ggcggcggtg atgatgccgc gcgttccggt ctcgacgatg tcctcgtccc agatgacatc
     1561 gtgcgggacc ggcggcgttt gcgcggaaac gctttgggca gcggcaagaa tacagaacag
     1621 gatgatcccg caccggcgct tcatcatcac actcccgccg ccgtgagatg atcggtgtgg
     1681 cgcgatccgc tcattccccg gcattaagat aaaaatgttg cgtttgtagc cggtcccagt
     1741 atgctgtcat gagcatgaaa atgtcaaaca catcttgcgc ggcagcagga ggcggcataa
     1801 tgacgatccg tacaataaca gcaaccatcc ttgcgcttgt cgctgcgttt tgcgcggcct
     1861 gcgcgcagcc gcgaacggcc gtcactgccg cgcccgagga cccgatccac ctgctgcgga
     1921 accactccat caacccgaaa tcgcccctca ccttccgcgt gaagatggcg cccgctccgg
     1981 ttctggatta tctccgcaaa ctcgaccagg cgccggagta ccagaactat ctggtgcgca
     2041 aaagcgacat ggggcagatc gcgacctacc tcaacctcat gccgtcgaag atgcgggcgg
     2101 tgctgcagga gcggcttatc ggcatctact tcatcaacaa cttcaagggg agcgggctca
     2161 cggagtgggt gcgggacgag cgcaacaact tttacgcatt catggtgttc aacccggaca
     2221 cgctcgcgcg cgacctgtcg agctggctca cctatcgcga gcgctcgtgc ttcatcgcga
     2281 acgatccgga catatccatc gaggtcgact gcgggaaaaa gcacaccggc ttcctatacg
     2341 tccttctgca cgaggccgcg catgtcgtcg actacgcgca gggcatcacc ccctatgtgg
     2401 aggactcgct caaatcgatc tcacccgcgc ggggggcgcg gaccccgttc gtcgagggcg
     2461 cctggaacgg gatccgcacg cccgcgtccc cgttcgattt cccgctgcgc gcatcgctca
     2521 ccttctacgg gttcggcggc gggccgaaga tcccgatcgc ccgcgccatc gacgcctacc
     2581 gcatgctcga aaagacgccg ttcgtgtcgc tctacggcgc gacctcctgg gcggaggacc
     2641 tggccgacta cgccttcttc atgcacctca ccggagcgct caaacagccc tacgccatca
     2701 ccgtgcggaa aaaggggtca gacccctata gtattcgccc ggcggaaacc gctcgaaaaa
     2761 ccctaaaccg cccctacccc ctgtaatcca aaaaaattct caaaaaaagc ttgccggttt
     2821 tcgcatatac tatacttttg tatagtattc aattccctct gctcttcggg caccgccccc
     2881 ttcgccgggg ggaggaacaa tatggggcac tgatgactgg aagcgccatg caaaccatga
     2941 acgatcgcgc gcgaagcgcc atctccgaaa tctatgcctg cgcgggaacg gccgggccgg
     3001 cccttccctt cctgctggcg agcatcgcgg ccgggttccc ctcacccgcc gacgactaca
     3061 tcgaccggat gctggacctg aacgaatttc tggtgcgcca tccggccgct accttcttcg
     3121 tgcgcgtggc gggcgagtcc atgatcgacg cggggatcca tcccggcgac atcctcatcg
     3181 tggatcgttc gatcgagccc gcggacgacc ggatcgtgat cgccgccctg aacggcgagc
     3241 tcacggtgaa gcgcatccgc atggaaagcg gccgcatttg cctcgcgccg gagaacccgc
     3301 gcttcccggc catcgagatc acgcccgaca tgcagttcga ggtgtggggc gtggtgtgcc
     3361 acgtgatcca tcgcgtctag gggggcggcc gtgaaaacga tcttcgcgct ggtggattgc
     3421 aacaacttct acgtctcctg cgagcgggtg ttcgatccgt cgctcgccgg gcgccccgtg
     3481 gtggtgctct cgaacaacga cggcaacgtg gtggcgcgct ccaacgaggc caaggcgctg
     3541 ggggtcccct tcgcggcgcc ggcccacgaa tgcgcgccgc tggtgaagcg gcacaacatg
     3601 gccgtcttct cctcgaacta cgcgctctac ggcgacatgt cgcagcgcgt catgagcatc
     3661 gtggccggct tcgcgcccga gatggagatc tattccatcg acgaggcctt cctgtcgctg
     3721 ggcgatctcg cgggcgtgga tccgctcgat tacgccgcgc gcattcgcga gaccgtgcgg
     3781 cgctggaccg gcatcccggt gtccgtcggc atcgccccca caaaaacgct cgcgaagctc
     3841 gccaatcgcc tggccaagcg cgacccggcc tgcggcggcg tgctcgcgct ggacggacgc
     3901 tccgacctgg actcgatact ggacggcgtc gccacggggg acgtgtgggg cgtggggccg
     3961 cgctacagcg cgatgctcgc ccggcacgga atccgcacgg cgcgtgagct gcgcgacgcc
     4021 agcgacgcct tcgtccgaaa acgcatgacc gtgacgggcc tgcgcaccgt gcacgagctc
     4081 cgcggccgct cctgcataga actggagggt gccgcgcccc cgaagaaggg catcgtgagc
     4141 tcgcgctcct tcgggcggcc ggtcgtggag ctcgccgagc tctccgaggc cgtggcctcg
     4201 tacacggcgc gggccgcgga gaagctgcgg tgccagcgat cggccgcgtc gctcatgtgc
     4261 gtgtttgtga gcaccaaccc gttccgtgac gacccgcagt actacaacat catcacgcgc
     4321 cgcctcccgg cgcccaccgc ggacaccgcg gagctcatcc gccacgcccg cgccgatctc
     4381 gagcgcatct accggcccgg cttccggtac aagaaaaccg gggtgttcct ttcggagatc
     4441 gtcccggcgg acagcctgca gctggacctc ttcgccgcgg cggaaccgtc ggaggagcgc
     4501 gcccgactca tggcgaccgt cgatatcatc aatgcgcgat tgggacgggg ggccgtgtac
     4561 tatgcggcgg agggagtccg caaggcatgg cggatgcggc aggagcggct ctcgcgccgc
     4621 ttcacgacgc gctgggacga gctgccggtg gcggttgcgg ggtgatcgat atatctgtcg
     4681 cttaaacgag gaggaatgat gggtattgaa acaacagttt gcatgagtct ggcgaacatc
     4741 gagcgcctgg gatgggcatc gcgggttacc ggtttgtcgc tcgcgcatat cgtgcgcgcg
     4801 cttcttgagc gcatgatgct cgataagaga gtgcggacgg ggctgtgggg gcgggtgcgg
     4861 tatcaggcgc ggggcgccga taacaatatg tgccggttga atgtggtttt tcgcgaggat
     4921 gtctacgagt acaatctcga catgcggaag gcgtacaaga tgtcggtttc gctgattctt
     4981 gcgattgcga tagaaagata tctcgattca gtcgtgcggg aactaagtgg tacgggaggg
     5041 agcaccgata actatcgttt caacagctac gttatttcga aagatatagt cgatggggtg
     5101 atttgttgga aatcctactg gggattccca acaaaaatcc ccgaactcat ccatctggaa
     5161 taaccgcgtc ccccggaagc cgctacttca gccggtccaa tccgaaacgc ttgctcagca
     5221 tccggtccag cgttttaagc ggaagcttac tctcgagaaa caatcgcaga gaatcattcc
     5281 ccaggcgaat caccggcggc accttttttc cctccagccg ggtgatgagc tccttcgaga
     5341 actcctcggc cgtggtgggg ttctgctgcg aggtcatggc gcgcgcctcg atgaattcct
     5401 tcacgggggc gtacaccgag cgctcgtagc gatcgaggcc cttcgcggcg gtttccccga
     5461 actgcgactt gatggagccg ggctgcacgg taaccacacg cacgccgaac ggcgcgagct
     5521 ccatgcgcag cgcgtcggag agcgaactca cggcggcctt ggacgcgcaa taggcgccgg
     5581 agaacggctg cgtgaggaat ccggaaacgc tgctgatgtt cacgatcgtc ccggagtgct
     5641 gcttgatcat gtgcggcgcc accgcctgcg tgaggcccac cagccccgtc acgttggtat
     5701 cgaactgccg gcggaaatcg tccatcgaaa gatccaccac cggccccatc agcgcatagc
     5761 cggcattatt tattaggata tcgatcttcc ccgcctggcg catcacgtgt ttcacgcaca
     5821 tctctatcga cttgggattg gtcacgtcga gcgagctgat gtcggcgcgc gacgagcgga
     5881 tatcgactgt cgaagcgcgc cgcgccgtcg cgaagacgta atacccgcgc cggtcgaact
     5941 cccgcgcgag cgcgagtcca attcccgacg aacatccgct aatgagcacc accgtgtctt
     6001 ttttcatgta gattctcctt ccggttcccg gcagacaatt gttgcgattc gtgcttcgct
     6061 atatatgctc gatgattcct cctgtcaata aatatcaaaa tagtattgaa cattttcccg
     6121 ccgcccgcca ccgtatcgaa cgtcgcggcc accatccttc agggagcagt gttgatgtta
     6181 accgacccca tcccatcgcg cgccgagtgc taccgactca tcgacgagca ccgcatgctt
     6241 cccaatatcc gcgagcattc ggagcgggtg atgctcgtcg cgctcgccat cgccgacaac
     6301 ctgcgcgccg gcatgcgtgt caaccgcgac ctcgtcgtcg ccgccgcgct cctgcacgac
     6361 atcgcaaaaa cgcgttcgct ttccacgaag gagaagcacg atatcaccgg cgccgacctg
     6421 ctgcgagacc tgggctatgc gcgggtcgcg gagatcgtcc gcgcgcacgt gttcttctcg
     6481 aatttcatcg aggacgggcc gctcgaggac cgcgagatcg tctactacgc cgacaagcgc
     6541 gtgatgcacg caaccgtcgt gagcgtccac gagcgcgtcg aggacctggt cgcgcgctac
     6601 ggaaagacgc cggagatcat cgagcggatc accgcgaaca agcgcctcat cctcgccatc
     6661 gaggggaaga tccagcgcgc catggcgatc gcgatcgacg aggccatcgc gaacatcgac
     6721 gaatgaaacc acgatactgc attctcgact gctacgtgga cgagccggcc tgcttcggcg
     6781 tgccgccgtt tctcgcgccg tatgcgcgct acatctacgg cgcgctggtc gatggcggcg
     6841 ccgcgcccga atcgatccga tacgccacaa tcgacaccct gcgcgcctcc ggctaccggc
     6901 tccatgatcg cttcgaccat gtcttcatga tcggcggcgc cgtggtgccg ggacgctacc
     6961 tcggcgcgcg catcggcacc acggacgaga tggccgctat cgcgcgcgaa aaccccgaga
     7021 ccccgttcgc gctgggcggg cttgccgcgc gcttcatgga cgcgaatgcg cagcgcaacc
     7081 tcaccgtcgt cgcgggcgat atcgagctct tcgcgcacgg aaccgcccgc ggagaagtgc
     7141 gccgcggact ccgcaccacg gacgagatcg cgcgatgggc gaggaccggc gccaatgtcg
     7201 tgcgccagca cccgcacttc cccgacatca tctgcgagat cgagacctac cgcggctgcc
     7261 cgcgccaggc ccactgctcc ttctgttccg agtccgtgca cggcgccgtt tcctttcggg
     7321 atggcggcga tatcctcgcc gaaatcgagc gcctcatcga tgccggcgcg agccgtttcc
     7381 gcctcgggcg gcaggccgac atcctgctgt atggatctcc tctccgtgat ttcgcgaatg
     7441 gcttcccgcg ccccgatcct gttcccgtta tcgaactctt cgagatgctt cgaaccatgc
     7501 gcgacgacgg ccgcatcgcg ctccttgccg tagacaacgc caatcccggg accatcgcga
     7561 acttcccgga ggaatcgggc cgaatccttt cggcaatcgc cgcgtccatc acgccgggcg
     7621 acacgctggc actcggtgtt gagtcgctgg acccggcgat catcgcgcgc aacaccctca
     7681 aggtggacgc ggcggccctc ctcgaagtcg tgcgcctggt caacgacatc tgcgggcatc
     7741 gcgtggacgg cattccggta ctgctgcccg gcatcaacct catccacggg ctcatcggcg
     7801 aaacgcccga gacgtttcga atcaacttcg aggggttgtg cgaaattcga gaccgcgggc
     7861 tcctgctccg gcgcaccaat atccggacgc tcctgccctt cccgggaacg ccagcggcgg
     7921 cgcggcgcgg cgccgaatca acctccgtgc ggaaccgcta cgagcactac cgcgaccgaa
     7981 tccgccgcga gatcgaccac gcaatgctcc aacgggtcta tcccgcgggc acggtgatcc
     8041 gcgatgcgcg cgtcgaggaa tcgcgggacg ggtactcgta cgcgcgtcag atcgcgagct
     8101 atccgatcac cgcgaaggtg ccgctcgcgc tcgagccggg agcggccgcc gacctcgtcg
     8161 tcgtggggca ccaggagcgc tcgcttgtcg cgctcccgct gccagtgcgc atcaacgcgc
     8221 tgccggcgac ggcgctctca cacctgccgg ggatcgggaa acgacgggcg tccgagattg
     8281 tgctgcggcg gcccttcact cggccggagg aggccgcgga cctcatcgcc gatgtgccgc
     8341 ccgcgcttcg ggcgcgcatc ggcgtgtagc gtggcgggtc agtacttttt acgctccttg
     8401 ttgtggttga ggcggttcat gatgtagatg gcggcatagg cgccgataaa cccggcgaac
     8461 acgttcacac cggcgtcgat cgcgaggaac ttgaggacct tgatggcgat gtcgagcagc
     8521 aaccggtcga gcacgagccc cccgatgagc gcgccgatga cgcccaccac gagccccccg
     8581 atatagccgc cgaagaggtc gcgttttttt aaataataaa aaatccacga aaccagcgcg
     8641 ccaagcccca gatagaggac caccgcgaga attttcatca ggacgctttt cagttcatcg
     8701 gacatgggaa ccccttttgt cgtgcatcgt ctttgttatt ggcatgcccc acacgcgcac
     8761 cgttgtcaaa taaaaaacag ctcgcccttt ccctcgcgga ccacccgcag cgcgccctcg
     8821 gaaaaatcga caaccgtgga cggcgtcgac accatgggac cgcagctcaa gacaagatcg
     8881 atctcgtgcc ggtactgttt ttcgatgtcc tcgggctgga cgatgtactc tcccgacgac
     8941 gcgctcacgc ttgccgaaag gatcggccgc cccaacgcct gcaccagcgc gagcgcgatc
     9001 cggctgtcgg gaacgcgcac gccgatcgtc ttctggttgg tgatcactat cttcggcacg
     9061 agcttggagg ccttgaagat gaaagtatac ggtcccggga aggccttctt catgatcttg
     9121 aaggcctcgt cggaaatgtt gcgaacgtat tcgtgcagcc cggagatatc ggagaagatg
     9181 aagctcagcg gcttgttctt gggtatgtgc ttgatgcggt agatgcgctc gatcgcatgg
     9241 cggtcgcgga tatcgcagcc gtaggcgtac acggtatcgg tcgggtacac gacaacgccg
     9301 ccctcctcga gcacggcgcg cgccttgtcg ataaagcgct tttgcgggtt ctcctcattg
     9361 atcacgaaaa tcatgctacc ggttccctgc tgagaaataa aaggcttttg caggagtgcc
     9421 gcctgcaaaa gccttcaacc tggtaattta caataggagt gtaaatttat gatcactcaa
     9481 tatatcggca gtttccgaaa tcgcatgagg cttttttagc gtttttttcg cgcgtgccga
     9541 tccgggcgcg cccggaaatt atattgaaaa aacggggggg ctgatgataa tggcggcgcg
     9601 gagcgcattc catgtcagag attgaactga aaatcaaaag ccgggacatc ggcaccctaa
     9661 tcgcgaccat caaggcgaac aatcccgcgg ccggcctcga cctcgtcatg aaggcctacg
     9721 agctcgccga ggccgcccat cgcggccagg tgcgcctctc cggcgagccc ttcatcatcc
     9781 atccgctgga agtcgccatc acgctggccg cgctcaaact cgacaccgac accatcgtgg
     9841 cggccctcct gcacgacacc gtcgaggaca ccacgatcac gctggagaag ctcaagcgcg
     9901 acttcaacga gaacatcgcc ctcctggtcg acggcgtcac caagatatcg tcgctcaaga
     9961 agaagtccaa gcacaccgag caggcccaga acctccgcaa gatgctcatg gccaccgtga
    10021 aggacgtccg cgtgatcctc atcaagctgg ccgacaagct gcacaacatg cgcaccatca
    10081 tgttccagcc gccggcgaag cagcgccagg tggccgagga ggtgctcgac atctacgccc
    10141 ccctcgcggg gcgcctgggc atctccgcgg tccgctccga gctcgaggac ctcgccttcc
    10201 acgtgctcaa tcacgacgac taccacgaga tcaaggagat ggtggcgcac cgaaagcacg
    10261 agatcgagga gttcatttcc ggcatccaga ccgtgctgaa gcagaagctc gccgagctcg
    10321 acatcgacgc cgacatcacc gggcgcgcga agcactatta ttccatctac cggaagatca
    10381 agctgcagaa gaaatcgttc gacgagatct tcgacatccg cgccatccgc atcatcaccg
    10441 acaagatcaa ggactgctac agcgtcctgg gcatcatcca tacgctgtgg acgcccatca
    10501 ccgcgcgctt caaggactac atcgccgtcc ccaagtccaa cctctaccag tcgctccaca
    10561 ccacggtcat gggcccgggc ggccatcccc tcgaggtgca gatacgcacc agcgagatgc
    10621 acgccgtggc cgagatgggc atcgctgcgc actggatcta taaggagagc gggcgcaaga
    10681 aggactaccg gaaccccacc atcctcaaga acatcaacat gtgggaatcc gaggagaaga
    10741 acacccgcga gttcatgaag gaactcaaga tggacctcta cgaggaagag atcttcgtgt
    10801 tcacgccgca gggaaagatc atcacgctgg ccaccggcgc cacgcccgtc gatttcgcct
    10861 acgcgatcca caccgaggtg ggcaacacca ccagcggcgc gcgcgtgaac aacgtgctcg
    10921 tgccgctcaa gagcgagctc aaaagcggcg acatcgtgga gatcctcacc agcaagaagg
    10981 cgcacccgtc cgaggcctgg ctcaagttcg taaagtcgtc caacgcgcgc tacaagatcc
    11041 gcaactggct gcgccagcag tccgagtcgc agaagaaggc cgagcacgcg gcgcacgacg
    11101 agcaccgccg caagaagacc gcggaggtga gcatcccgga gcccgagctc atcaaggtga
    11161 aaaagctctc cgggcactcg aaggccgtcc tctcggtgga cggcacctcc aatgtgatga
    11221 tccggctctc gcagtgctgc cagccgatcc ccggcgacga cgtcatcggt ttcatcacgc
    11281 gcggccgcgg catcaccatc caccgcaaaa cctgcccgtc gctcgcgcgc ctgcgcctcg
    11341 agaaggagcg cttcatctcg atcgtctggg aggactccga gaccacctac cccgtcaagc
    11401 tctccgtgac ggccttcgac cgccccaatc tgctcaagga cctcgccgag gagatcggcc
    11461 tggccaaggc caacatcctc aaggccgagg cgcaggtgat cgagaagggg tccgcccggt
    11521 tcaagtgcac cctcgaggtg aagagcctgg cgcaccttca ggacatcatc gcccgcatca
    11581 agaagatcaa gaacgtgacg agcgtctaca aggtcaacga aaaggtcgtc atcaagtgac
    11641 gcgatccacg cagcgcgacc gcgacagccg cccgcttcga atcgtgctgg gatcgtcgtc
    11701 gccgcgccgt cgcgagatcc tgggcgcgct gctcgacgga cgcatcgaga tacgccaccc
    11761 cgaggcggac gagcgcgtcg ctcccggcga gccgcccgcg gactacgccc gacgcgtcgc
    11821 gcacgcgaag gcccagagcg tgtgctcgtc cctcggcccg ccggacggga gcgacgtcct
    11881 cgtgatcgcc agcgacacca tcgtgaccat cgacgggcga atcatcggca agcccaatga
    11941 ccgcgcggac gccgagcgca tactcgcatc gctcgtgggg cgcacccacc aggtgatcag
    12001 cggcctcacg ctggcgcacc gtccggcgct cggcgcgccc gatagctgga acatgcacac
    12061 cgggctcgag gccacggagg tcaccttctt tccgctttcg cccgatggaa tagcgcgcta
    12121 tctggattgc atcgattacg cggacaaggc cggggcctac gccctgcagg aaaacggcgg
    12181 gatgatcgtc cgaagcgtga acggctcgat gaccaatgtc atcgggttcc cgcttcggct
    12241 gtttttcggt atgatttcgg cgctgacggg cgccgatgcc gtgtttattt cttcttctcg
    12301 cgcgccttga gcgcctcgac catcttcgtg aaaagattgt agaagtcctg gagctcgtcg
    12361 ccctgccgca gcttgcgcgg attgggcatc ttgccgtcga tgatgtcggc gaaatacttc
    12421 gacatgacga agatcgggcc ggagatcttg tgcgtcatgc ggatcatcac gaaatacatg
    12481 atcacgctct gcaataacac gatgaccacg atcaggccga tgaggatggt attgtagcgg
    12541 atgatccgct tgatggtggt caggttttcg aagtgcttgc cggcgatcgc cttgatcgct
    12601 gtctgttgcg gcttctccgc ggggttttgc acccacgcca tcatcgcgtc cacgatattg
    12661 tcctggatgc ccatgatatt gtcgagccgg ttgttgttgt aggcggcgtt cacgccgatg
    12721 atcccgatga tgacggccac gagcaccaat accacgccga ggacggaaaa ggtgtgcttg
    12781 agctggaact ttctatcgat aacatattgc tttcgctgtt tcattggagc attcccctat
    12841 cttacgttgt tcttgtacac tgctacgcgt caacctgcga acgccgcatt ccctcaactc
    12901 ttcgggacca tcttgatcag cgactccacg atctcggcca ccacggggtc tttcgaattg
    12961 cggcagtaca ccgagtacga caccaccggc gatatctgcg tatagaagat cttatagtaa
    13021 taggtcgcga agatcaggtc gtatcggccc agcttctttc cgaagaagaa ctcgctcatc
    13081 acggtcgacg ggcggtccgg atcgtagatc ttgacattat agttgtaatc cgccctgaaa
    13141 atgtcacgcc ctttttcgat ccgcgacttg agcaggtcga gcgagctcat tttgtacgta
    13201 tagttgttcc tgaagatgga cccctcgatc acctcgcgct tgaagtcgtt caggttcgtg
    13261 aagaatttcg tggtcggtcg agcgggattg acgaattttt tgctgtcggg gaaatactcg
    13321 atcacgcgat cgatctttcc gtcgctgccg tcgtccatga cgagagaaaa catcttgtcg
    13381 ctgccgacga aatcgccaat gaggatattg gtgttgtcgt cgatggactg gaccacgaaa
    13441 taattacgga agtcccccgg caccggcaag aactgcatcc gcgccttggt gaacggctcg
    13501 gtcgccatga atccatgctt gaattcggtt tgcgcgaaca acacggtcat gccaaggacg
    13561 cacaaccctg caatgctaac ggcaacaacc aatcgtgtct tcttcatacg tgttcgctcg
    13621 cttatttcat atttgatttt gctcaaggaa tatacatact gtatcggcac caattcaata
    13681 ctttttcatt tttttgttga aaaaaaacga cggaattata gcctgtcaac gattgcgggt
    13741 gtaattcagt ggtagaatgt cagcttccca agctgaacgt cgagggttcg agtcccttca
    13801 cccgctcaga tgcccgacac agtcgagggc gcagtgttcg ctgcgccctc gttgcgtccg
    13861 aacccgaacg caatacgctc accacatgcc ccgcaccgcc gacaccagcc ggaatcgcag
    13921 caggcgcgca taaaacgcca gccggctccc gaagccgagc gttatcaccg taaccagcgc
    13981 cgtgaccgta tacatgaaga cggccgcaaa gcgcgcctgg atccggcgaa gcgtatcatc
    14041 aagcgcggcg agcaccggcg aatcggacgg cggcgtctgc acggtgaagc gcacgtatgc
    14101 gggcgcagtc cagcgggtct cggcgcgccc gtcgatgact gcggagtagc gcgccttcac
    14161 cgcgagatag tactcgccgt ccgacaggtc gcgcacgcgc aggatcgcgc cccgcaggtt
    14221 caccgcgtcc ggcggcgggg tccgcgtatt cgtgaccgca taggcgtatc ccaggaccga
    14281 tccagccgcg atattgcggg gggaaaccag cgcgtcgaat tccgcgcggg cgagcgggcg
    14341 cttcgcatcg aagggaagcg taacggccac atgggggacg ataacgcccg ggacgcgccc
    14401 gggcgtcggt tccttcgtcg cgctgccgtc ccgggccatc cgctcgtaca tcgacggatc
    14461 cagcggcgcg gcctcgccga cgatgatctc ataggcggcg gtgcggctga aggtgttggt
    14521 ggcgtcgacg gatcggacct tgaagaaata gcgtccgttg gcgaggttcg agaagttcat
    14581 ggacagattc gtggtaaact ggtccggcgc cttgagcgga tcattcgaaa acgaaaacac
    14641 gaaccccttg aggcgctcgc cctcgtcggt cgaccactgg aacgtcggcg acgggctggg
    14701 gacgggggtc ccctccgggt gcgtgggcga tacgacgacc ggcgccgcgg gcgggttgcg
    14761 gctcacctgc agccggaaat ggatggtccg gctgaagttc ccggcgccat caaccgtcct
    14821 gatgtgaaag taattgaccc cgtcctcgag gtcgctcagg gtcatcgact ggaaattagc
    14881 gtcaaggttc ctgatggtgg gattgaacac cgggaggcgg ttcacgatgg tcgcatagcc
    14941 caggatgccg gactcgtcgg cgggcgggcg ccagttgatc acggccgtcg acgccttgga
    15001 ccagaccccc tcggggtgcg tgcccgaaaa caccaccggc ggctccgcgg acacgtcgga
    15061 gtattttgcg atgatgcgcg ccccctcctc ccagaagagg accaccatgg gaccgacgga
    15121 cgccgccgcg ggacggcgcg cgaccgacgc cgcgccggag agccgcaact ccggcgagag
    15181 cttccggtcc tcgatggaat agcgccgccc gtagatcccg gtattggcct ggcgggtgtc
    15241 gtgccaggcc acgacgatct cgtcgttgcg cgtcgccacg atcgcgggcg aatagcagtt
    15301 cgcattggtg tcggagatgc gcacgggact gcgctcccat tcctcgccgt cgttgaatcc
    15361 gcgcaccatc gcgatcgacc agttcttggt ctcgttcgtc tgatacgcca gatagagaat
    15421 gcccttgtag accgcgagcg acggcgcggc ctcgttcgcc gtgctctcgg tgatgcggac
    15481 gcttccggtc cagctccgcc catgattgct cgacttgatc aggaagagat cgtcggagag
    15541 cactcccagc ttctcgccct tcccctgcca cacgatgtag atggtggacc cggacacatt
    15601 gatcgacggg agaaaggcgc cgcgcagccc cttgtccacg tccacgagcg catcgccgac
    15661 atcgaagaga ttttcgtccg ggctcttcga gtggaagaag gtgagccgcc cgccgaggaa
    15721 ggcgtgatag aacaggtgca gcgcccccag ctcgtcgtaa atcacctgcg ccagcatctc
    15781 catctcgatg ggagcggcgt actgcgtgaa gatccggcgc ggctccgacc aggtggcccc
    15841 catgtccttt gactgcgaga tgaacagccg ggtcttcgag tcctccgcct gcaggttctg
    15901 ccagatcacg gccacatgcc ccgcgccgga gatggcaacg tgcggattcg cgtccacctt
    15961 cgaaagcacc tcggcgatgc gcacgggcgc gatgtagctc ctcccgccgt tgaacgaaat
    16021 cgaaacgtag agcgtcttgc ggtcgccctc gagcgcctcg tagaccgcgg cgaggtagtt
    16081 ccccttcgcc acggcggccg cgttgcgcgc attgagcgcc gggggcgaga cgaggacgct
    16141 cctttcccag ccgacgggcg cactctccac cgggccaaac gcgtagaacg ccgcgacgcc
    16201 gagcgccgtc aggagcgcgg ccatcatccc ccagatgagg tgccgcacga aggcgtaccg
    16261 gtaacagaaa tcgaccagga gggcaagcag gctgttcatg tcgggccgtt tagaacatca
    16321 cgtatataaa gggcgacacc tcgtcgtcga tctcctcggg cgcgtacagg agcccggcgc
    16381 acgcggccat ctccggcgca cacttcgcca gctcggggtt cccggaacgc tcccagagcg
    16441 cggcgagttt tttcccgaac tcgttctcgt cggcgcacac cggcgcctcg aaatcgcccc
    16501 gcgacatccg cgtcgcgttg cgcgcgacat agtacgtcgc ggccgagggg tccgcgccca
    16561 tcgggaaata cgcggccccc ctacgggcca tatcggacaa tggcaggagt ttccggaaga
    16621 acaatttctt cacccgcgcg cgggccttcc cgccgtcaat cgagctcata gtccttcctc
    16681 cagtcgccgg cctccgcgaa ggcgggctgg ttgctcttcc acaacaggca gtcctccagc
    16741 accaggaggt ccatctcggt tcgcatgaag caccggtagg cgtccgcggg ggaacacacg
    16801 atgggctcgc cgcgaacatt aaaaga
//
LOCUS       DAORHF010000052        16598 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2569_length_16598_cov_5.062202, whole genome shotgun sequence.
ACCESSION   DAORHF010000052 DAORHF010000000
VERSION     DAORHF010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16598
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2569_length_16598_cov_5.062202"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="PKJ16_06450"
     CDS             complement(<1..1007)
                     /locus_tag="PKJ16_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HNV46664.1"
                     /translation="MPKNITKVVLSDDEIPRQWYNLAADLPTPMQPPLGPDGNPISPD
                     MLAPVFPMNLIEQEVSSQRWIDIPEEILEILYRWRPSPLYRAKALEKAIGTPARIYYK
                     NEGVSPAGSHKPNTAVAQAWYNKQFGIKRICTETGAGQWGSALAFSCALLGLECKVFM
                     VRISFDQKPFRKLMMQTWGANCVASPSTETNAGRNILKESPDTPGSLGIAISEAIEAA
                     VSDTSGKSRYSLGSVLNHVLLHQTIIGLEAKKQLEVFGEKKVDVVIGCAGGGSNFAGL
                     AFPFVADKINGANIDIIPVEPASCPTLTKGPFAYDFGDVASTTPLLPMHTLGHAIIPE
                     P"
     gene            complement(1236..1994)
                     /locus_tag="PKJ16_06455"
     CDS             complement(1236..1994)
                     /locus_tag="PKJ16_06455"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HNV46665.1"
                     /translation="MDELVRKAALAIMGSVNCIALTGAGISVESGIPDFRSAGGLWSK
                     YDPMEYAHITAFQRNPEKIWRMIFDLLDLTRSARPNPAHQALAALERMNYLKSIVTQN
                     IDNLHQAAGNENVIEFHGNAHRLECLECGARYDINDFELAGVPPACRSCGTILKPSVV
                     FFGEMIPHDALVNAQILAQNADAILVIGTSAVVYPASGIPYIARDHGAVVIEMNIEET
                     ELTGSITDIFIQGPVGETLPALLSMIEMESKKKS"
     gene            2180..3817
                     /gene="glpA"
                     /locus_tag="PKJ16_06460"
     CDS             2180..3817
                     /gene="glpA"
                     /locus_tag="PKJ16_06460"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017051888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HNV46666.1"
                     /translation="MTKNEFDVIIIGGGATGAGIVRDCSLRGLRALLIEKDDIASGTT
                     GRNHGLLHSGARYAVKDLESARECIRENRILKKIARHCIEDTGGLFVTLPDDDPDYHN
                     RLLEHCKYADIDREEISVSQALAMEPNLNENIMAAIRVPDGTIDPFRLSSSNILDAQE
                     HGARVATHTIVTGLIKNGANIAGVTCRDLRTGEEKTFGSSIIVNAAGVWGQRICAMAG
                     IELAMYPSKGSMCIIDYRINNVVVNRSRMPADGDIIVPGDTVSIIGTTSKRIDYEDIE
                     KLTVDDDEIDVLLADGEKLIPNVSKTRVLRAYCGVRPLVAASGESTGRDISRGIVLID
                     HASRDGVEGIITIAGGKLMTYRLMAQETTDLVCRKLNVKKRCVTHRRALPGSEKKTSY
                     RKRIKLFTGIAESVIGSTHYRHGERVRSILKGEKKNYGLICECEMVTAGEIEYALEKL
                     NVKDIIDLRRRTRIGMGPCQGELCSYRVAGLFNELGRADAAESNRFLIEFLEERWKGI
                     KPILWGDALREIEFTYWIYQGLFGLGDLKEPKEGEDR"
     gene            3814..5115
                     /gene="glpB"
                     /locus_tag="PKJ16_06465"
     CDS             3814..5115
                     /gene="glpB"
                     /locus_tag="PKJ16_06465"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HNV46667.1"
                     /translation="MKYDVIIIGGGIAGLTCGIGCAEAGLSCAIISAGMSALHFSSGS
                     IDFLGYRDDGEIVENPHAYLDEFIAHHPRHPYAKCGAETVRSSLSFIRDQLSRERLEL
                     FNNGEENHFHVTALGTLKPTFFSQRSVFNERIREAFTSHRKIAILNFRGFRDFYPAMT
                     ADNLRKNSLFKDIEIIAGTITLPAPRTPGRNPFEFRSIDIARIFDSEKYLPRIADEIR
                     RAADGARLIGLPAFIGITRYNKIHERLEELTGALIYEIPTLPPSILGMRIDTALKTRF
                     AALGGVFISGDRVNGGEIADGALDHIHTVNHRTMRHHARWYVLASGHFFSGGNVSEVT
                     GIREPIWGLSVRGPVDRSAWRSPEFFSAHSHPFLEYGVITDESFRPMDPGGATVRNLF
                     CVGAVLADYNPIREGTGGGVAVASGYAAARRIIDSITGAAS"
     gene            5112..6311
                     /gene="glpC"
                     /locus_tag="PKJ16_06470"
     CDS             5112..6311
                     /gene="glpC"
                     /locus_tag="PKJ16_06470"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HNV46668.1"
                     /translation="MIKLENISFDHCIKCTVCTIYCPVARATHLFPGPKQSGPDTERM
                     RIKSPELVDASLKYCTNCKRCEIVCPSDVKIADIIQQARWNYLKKKFRIRDFFLSRTD
                     LVGGMMTRLSAIANFFISLFPVKLMMTLVLKIPYQRVFPRYEQGTFRKRFRSIAAAQR
                     AFDRRVVYFHGCFVNYNNHALGFTLVRVLNAFGIGVEIADEKCCGVPLIANGYIEQAK
                     RNGAYNIAHLSRATRDRELTVVSTSSSCTFALKYEYSHLLELDNSDIFPRIEYITKYV
                     FSLLEEAAAPPLKPVGLRAAYHAPCHLERMGGVPYTIEVLRRIPGLDLVILHSECCGI
                     AGTYGFKSEYYAISQNIGSDLFAMIERAEPEVVITDCETCAMQIAMNTPYRVLHPLDV
                     LAMSLGE"
     gene            6334..6753
                     /locus_tag="PKJ16_06475"
     CDS             6334..6753
                     /locus_tag="PKJ16_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV46669.1"
                     /translation="MKSHTEYLTFTTASRRDYLNITRTIEGIVAASGIREGLCLVNAM
                     HITASVFINDDESGLLQDFDDFLERLAPHEPIGQYRHNRTGEDNGDAHIKRTVMGREV
                     LVAVTNGRLDFGPWEQIYYGEFDGRRPKKVLVKVIGE"
     gene            6756..7529
                     /locus_tag="PKJ16_06480"
     CDS             6756..7529
                     /locus_tag="PKJ16_06480"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HNV46670.1"
                     /translation="MRLVDVHCHLEDPVFDDRLDDIITDARRAGIARIITASVVPSDF
                     AKSRSIAARYPEVSFALGIHPWYVTETSERDADSLSAARDLGAVAIGEIGLDRKIDAP
                     DFELQRRIFEKQLAIANELSLPVVLHCRGAFSELVESLKRVGRPVGGGIIHAFSGSVE
                     IAERCIAMNLSFSMGGTLTYRNSRKRETVVRRIYPDHFMLETDSPDIPPVECREKPNV
                     PSNILYNLAAAAETLGRSREEVALRTTQTAERIFGLPPS"
     gene            7653..8165
                     /locus_tag="PKJ16_06485"
     CDS             7653..8165
                     /locus_tag="PKJ16_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46671.1"
                     /translation="MNKILAAIIITIAFGCAAFAQTASIPLVEEDREFWNDILRDESI
                     KNKNLLYASFVAYKDRLNDLSLETFRESMKNNQGNDIVQCLAQYYIGKNLIALGKLPE
                     GLAQLQTAGSLPCPRVEHLKYAIRINTAIAHHRLRNFREFNRILTEIVNDDSAGRYRS
                     IARGVLTLER"
     gene            complement(8172..9500)
                     /locus_tag="PKJ16_06490"
     CDS             complement(8172..9500)
                     /locus_tag="PKJ16_06490"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HNV46672.1"
                     /translation="MKVTVVGTGYVGLVTGTCFAEMGNDVWCVDIDAKKIENLKNGII
                     PIYEPGLEEMVARNFSEGRLHFTTDIKECIDETLFVFIAVGTPPDEDGSADLQHVIAV
                     AREVGRLINKYTIVVNKSTVPVGTGEKVRAAVREELEKRGRGEVEFDIVSNPEFLKEG
                     NAIEDFIRPDRIVVGVDNVRTGELMRELYAPFVRNGHPIFTMDILSSELTKYAANAML
                     ATRISFMNEISRLCEAAGADIEMIRQGIGSDSRIGMPFLYAGLGYGGSCFPKDVKAIV
                     QTAKEFGVTSQILDAVEDVNKQQRELFVQKVLAYYGGTVKGLTFAVWGLSFKPQTDDM
                     REAPAIDIIHALREKGATFRAHDPVAMEIASEIIGKNGVEYVSNYYDALKGADALLLL
                     TEWHQFRHPDFAKMESLLKKKVVFDGRNQYDPAKMKELGFEYSCIGRPKR"
     gene            complement(9497..10303)
                     /locus_tag="PKJ16_06495"
     CDS             complement(9497..10303)
                     /locus_tag="PKJ16_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HNV46673.1"
                     /translation="MFTIKKLLLCIITIPGALIALMFAGGILAVRKRRYGGALLMASA
                     AALYILSIGPVADFAIGRIELLNHATAHDNADSIILLGGGLVEGAADLTGEHIPSPVM
                     MVRIVECVRLHRRHGLPIVVSGGVVGSGPAEAPVYRRFLVDLGVPPERIIVESRSRDT
                     VENARYVRPIIAERGFRTSILLTSAFHLRRATLIFREEGLSVVPHSCGQLARSKKKLD
                     TYDALPTMHNLYKSSLALREFAGLALYRIKYAVAPSSSTRTTIVSIRRDQ"
     gene            complement(10358..11008)
                     /locus_tag="PKJ16_06500"
     CDS             complement(10358..11008)
                     /locus_tag="PKJ16_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV46674.1"
                     /translation="MILDGVDAVFDEGSIVSLTGPSGSGKTTLLGVIAGMLKPDRGTV
                     TYKGRNIFRMGDLRRSRLRNREIGYVFQSFNLISDISAYQNIVYPALINRTARDISQR
                     VRHLIEYLDLGGIIRQYPASLSGGEKQRVAIARAMINRPSVILADEPTGNLDEANGTK
                     IFSLFRDLAREQGVLFIVATHDKNIIQYSDRHFHVENGRCADATPVPVKRHRKATS"
     gene            complement(11119..11826)
                     /locus_tag="PKJ16_06505"
     CDS             complement(11119..11826)
                     /locus_tag="PKJ16_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46675.1"
                     /translation="MRTRYIVGLVILLVMAMALSFSPAAAQEKKADQDSLESKELAEE
                     GTALDKQIADLSKKISEVIATYELANKANRFKTLPYQMSLIRGDGFIEIERHRFTRSN
                     VDNTRITGIQKKKLKIGTDGKSANKLEFIVWERNYDTEAETQVLVTDASPATPDKKGM
                     KFDYSVNKRLLKSMTLDQIQNTTAFPIRNELKRDFVIPHLTYFYNSLLMIGETYRKGM
                     KDSESLMADFLKESTKY"
     gene            12031..12747
                     /locus_tag="PKJ16_06510"
     CDS             12031..12747
                     /locus_tag="PKJ16_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46676.1"
                     /translation="MKRLIHITLAIALLSSVPTWLSAKKAPSADDELMKKAIAYFYSR
                     KFEMSELLFQEALKKNPENATAYSYLGDIFLQKKRYDGALQLYKRALDLNPENGDEYF
                     RVGQIYYYKRQSEDAIVNFKRAYELNPKLKFALYHIGLTYLMLARDKQNTIVNWESYL
                     AAAPEDPQYEKLRRAVELLKDRNFIIPKEGSDTSIEEALHLGGMALQRQPRAIEDRKA
                     DHEDKKTKYKLEDIERDDGL"
     gene            12776..15184
                     /locus_tag="PKJ16_06515"
     CDS             12776..15184
                     /locus_tag="PKJ16_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46677.1"
                     /translation="MTLYRSDTSHAVKFGIVALVAALIIGGGIYLFQSRDRGVDEVSD
                     LRLRQIELYKKLLDTEPSSIPLLIKIADLYKQVGNLDAAIDHYRKALAIDPMNYDALN
                     GLGHAYALKGEYDKAIETFERAKKSGPRLPRAYNRLGNVYDEINKEQQAFTEYKTAKR
                     VAPKDQESYYNLARQRLKKGDTRNAIKYIKEGMKANPRDAEGHYNLGRLYLDTKQPDM
                     ARRSFAKAISINPRVAKYHRDKGEAELQLGKNSEAASSFIKTLEIDPTDARAAERLGD
                     LAARDANWAGAAGYYKKALEYNHNDTNLQQKYRNALAMLDRKKDIAKKVDPETPAVAT
                     DDPERPVIKKDDRAMAPETDTKDAIAKDDSARKKPDAKENDIKPSARQYRDLGDKYRA
                     QKDFDNATKSYLKATGIDPSDDHSHYWLGRIYYHNKMDDEALKSFQKATAANPKNDDA
                     HYRMGLIYYQQKKYSAAVQSFSKAIAIQPNSPKAFYARGLANYKLNDKGQAIKDLKTA
                     ISQDPRMDVAYFNLGSIYIDNKNYAEALKYFSKDRELRPNNPETHFKIGEIYHEMKSL
                     NSAVQSYRKTIELDPTHYQANFNLGLVLSRQKNYPAAIKQFERVLANKPDDYASMYEL
                     GKAYEGTGNDNRAIEYYSKAIARNPAYSKAYINLGGLYRKNKLFDKAIEQFSNAVSNN
                     PKSFEAHYNLANAYRETKMYDQSIDEYKIAITLRPMSSQAHFALGEAYRDKGSFEDAA
                     REFELALQYNNKLLVAHEELGLIYRKMKNTDKAVYHFERLLSARPNHPDADKIQEMIN
                     LMKK"
     gene            15272..15796
                     /locus_tag="PKJ16_06520"
     CDS             15272..15796
                     /locus_tag="PKJ16_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46678.1"
                     /translation="MKKFSSNIFGRCALGAGAIILLLCAPMTSCIIRAYDVDRLKSDY
                     KTEGFLDADHFQVIVVGTPDKDKRGLVQKRESALENAKNKLSKTVIDKLSEYYLSVHL
                     PTLGIGSAKEIANFDDVRVNLTHELMPFLNDGSVAFEYYNYDHSAVIVFRVIRRNLQR
                     ALASVVLPVAIQKK"
     gene            15809..16297
                     /locus_tag="PKJ16_06525"
     CDS             15809..16297
                     /locus_tag="PKJ16_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46679.1"
                     /translation="MKFNAITIPAGPAPRALCAIAIAASLALSHCSTKSTSAVTKPTH
                     GGNANLSDRDVLPAEYQREGFINKDLYRIVIVSPADECPDMSYVEKNARQRAFMSLQK
                     HLESRNKIVDQNTRARLLNLINEKGTVKNLECRDEKRKAFVFEIEMKNLGQYLDSLAG
                     QR"
     gene            complement(16294..>16598)
                     /locus_tag="PKJ16_06530"
     CDS             complement(16294..>16598)
                     /locus_tag="PKJ16_06530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV46680.1"
                     /translation="TIVNISRGGFYFFAPRAVPVSSILRVTIDFSGFDPGLGSIEALA
                     MVMRSEPKGRGRFGIGVMFSSIYDDNRESLELFIFKHLAYRMHASQDAETGPADGR"
BASE COUNT         3525 a         5221 c         4740 g         3112 t
ORIGIN      
        1 atcggctcgg ggatgatcgc atgccccagg gtgtgcatcg gcaacagggg cgtcgtcgag
       61 gccacgtcgc cgaagtcgta ggcgaagggc cccttggtaa gcgtgggaca cgaggccggc
      121 tccaccggga tgatgtcgat gttcgcgccg ttgatcttgt cggcgacgaa cgggaacgcg
      181 aggccggcga aattgctgcc gccgcccgcg cagccgatca ccacatcgac ctttttttcg
      241 ccgaatactt cgagctgttt tttcgcctcc aggccgatga tggtctggtg caggaggacg
      301 tggttgagca cgctccctag cgaatagcgg ctcttgccgc tggtatctga caccgccgcc
      361 tcgatcgcct cgctgatggc gatcccgagg ctgcccggcg tatccgggga ctccttgaga
      421 atgttgcgtc cggcattggt ctcagtgctg gggctggcca cgcaattcgc cccccaggtc
      481 tgcatcatga gcttgcggaa cggcttctgg tcgaagctga tacgcaccat gaagaccttg
      541 cactcgagac ccaggagcgc acaggagaag gcgagcgcgc ttccccattg ccccgcgccg
      601 gtctccgtgc agatccgctt gatgccgaat tgcttgttat accatgcctg cgccaccgcg
      661 gtgttgggct tgtggcttcc ggccggactc acgccctcgt tcttgtagta gatgcgcgcc
      721 ggggtcccga tcgccttctc gagcgccttc gcgcgataga gcggagacgg gcgccaccgg
      781 tagagtatct ccaggatttc ctcggggatg tcgatccagc gctgagagct cacctcctgc
      841 tcgatcaggt tcatggggaa cacgggagcg agcatatcgg ggctgatggg gttcccgtcc
      901 gggcccagcg ggggctgcat cggcgtggga aggtcggcgg cgagattgta ccactggcgc
      961 ggaatctcat cgtccgacaa tacgaccttg gtgatgtttt tcggcataac gccctccatt
     1021 cacagtttca tattcggcat aaaaaagggc tgcgatgtat tgccatccgc agcccttccc
     1081 ttgttttgca atttcggtcg agtaatcgat caagcgcgcg gatgatgtga tccgccgcgc
     1141 cacccccaag cctgccacgc attgaagcgt tcgtagtgtg tgttcgcttt catgtccatc
     1201 ttcggttcga tagcatagat actatcgtca ttctgtcaag attttttttt cgattccatt
     1261 tcgatcatcg aaagaagcgc cggaagggtc tcgccaacgg gcccctggat gaagatgtcc
     1321 gtgatggagc cggtgagctc cgtctcctcg atgttcatct cgatgacgac ggccccgtga
     1381 tcgcgcgcga tataggggat accgctggcc gggtacacga ccgccgaggt gccgatcacc
     1441 agtatcgcgt cggcgttctg cgcaaggatc tgcgcgttca cgagcgcgtc gtgcggtatc
     1501 atctccccga agaaaaccac cgacggcttg agaatagtgc cgcagctccg gcacgcgggg
     1561 ggcacgccag ccaactcgaa atcgttaata tcgtaccgcg cgccgcactc aaggcactcg
     1621 agccgatggg cgtttccgtg gaactcgatc acgttctcgt tcccggccgc ctgatggaga
     1681 ttgtcgatgt tctgcgtgac gatcgacttc aggtagttca tgcgctcgag ggccgcaagg
     1741 gcctggtgcg ccggattcgg ccgcgccgac cgcgtgagat cgagcagatc gaagatcatc
     1801 cgccatatct tttcgggatt gcgctggaag gcggtgatgt gggcgtattc catcggatcg
     1861 tacttgctcc acagccctcc ggcgcttcga aagtcgggaa tgccgctttc gaccgaaatg
     1921 ccggcgcccg tgagcgcgat acaattcacg gaccccatga tcgccaacgc cgctttgcgc
     1981 accagttcgt ccattatacc ctcgcaccgc tgtacggcgg ttcatgttga tgatgatgct
     2041 cggcgaacac cataccgacg tcaagtgaaa aagcccgcat ccgttaccgc acccataccg
     2101 ggcagccgcc gtattatgat tgacaggcga cccggtttcc aaactctaga tcaacggacc
     2161 ggagagggcg aacgcataca tgacgaaaaa cgaattcgac gtcatcatca tcgggggcgg
     2221 cgcgacgggc gcgggcatcg tgcgcgactg ctccctgcgc gggctgcgcg ccctcctcat
     2281 cgaaaaggac gatatcgcct ccgggaccac cgggcgcaac cacggcctgc tccactccgg
     2341 cgcgcgctat gcggtcaagg acctcgaatc ggcgcgcgag tgcatccgcg aaaaccgcat
     2401 cctgaaaaaa atcgcgcgcc attgcatcga ggacacgggc ggcctcttcg tgaccctacc
     2461 cgacgacgat cccgactacc acaaccggct gctcgagcat tgcaaatacg ccgacatcga
     2521 ccgtgaggag atcagcgttt cacaggcgct ggcgatggag cccaatctga acgagaacat
     2581 catggccgcc atccgcgttc ccgacggcac catagacccc tttcgcctgt cgtcctcgaa
     2641 catcctcgac gcccaggagc acggcgcccg ggtggccacg cataccatcg tcaccggcct
     2701 tatcaaaaac ggcgcgaata ttgccggcgt aacctgccgc gacctgcgga cgggagagga
     2761 aaagacgttt ggctcttcca ttatcgtcaa cgccgcaggg gtatggggcc agcgcatctg
     2821 cgccatggcg ggcattgaac tcgcgatgta tccctcgaag ggctccatgt gcatcatcga
     2881 ctatcgcatc aacaatgtag tcgtcaaccg atcgcgaatg ccggccgacg gcgacatcat
     2941 cgtccccggc gacaccgtct ccatcatcgg gaccacgtcc aagcggatcg actacgagga
     3001 catcgagaag ctcaccgtcg acgacgacga gatcgacgtg ctccttgccg acggcgaaaa
     3061 gctcatcccc aacgtgtcca agacgcgggt gctgcgcgca tactgcggcg ttcgcccgct
     3121 cgtggccgcc tcgggcgaat ccacgggccg cgacatcagc cgcggcatcg tgctcatcga
     3181 ccacgccagc cgcgacggcg tcgagggcat catcaccatc gccggcggca agctgatgac
     3241 ctatcgcctc atggcccagg agaccaccga tctggtgtgc cggaagctga acgtgaaaaa
     3301 gcggtgcgtg acccaccgcc gcgcgctccc cggatcggag aaaaaaacgt cgtaccgcaa
     3361 gcggatcaag ctgttcacgg ggatcgcgga gtcggtcatc ggctccaccc actaccgtca
     3421 cggcgagcgc gtccgctcca tcctcaaggg cgagaagaaa aattacgggc tcatctgcga
     3481 atgcgagatg gtgaccgcgg gcgagataga gtatgcgctc gagaagctca acgtgaagga
     3541 tatcatcgac ctccggcgcc ggacgcgcat cggcatgggc ccctgccagg gcgagctgtg
     3601 ctcgtaccgg gtggcggggc tgttcaacga actcggacgc gccgacgcgg ctgaatcgaa
     3661 ccgcttcctg atcgagttcc tcgaggagcg ctggaagggc atcaagccga ttctctgggg
     3721 cgacgccctg cgcgagatcg agttcaccta ctggatctac caggggctct tcggcctcgg
     3781 cgacctcaaa gaaccgaagg agggagagga ccggtgaagt acgacgtcat catcatcggc
     3841 ggcggcatcg ccgggctcac ctgcggcatc ggctgcgcgg aggcggggct ctcctgcgcc
     3901 atcatctcgg ccggaatgag cgccctccac ttttcctccg gctccatcga ctttctgggc
     3961 taccgggacg acggcgagat cgtcgagaac ccgcatgcct atctcgacga attcatcgcg
     4021 caccatccgc gccatcccta cgcgaagtgc ggcgccgaga cggtgcgctc atcgctctcg
     4081 tttatccgcg accagctttc ccgggagcgc ctggagctgt tcaacaatgg cgaggaaaac
     4141 catttccatg tgacggcgct gggaacgctc aagccgacct ttttttcgca gcgcagcgtg
     4201 ttcaatgagc ggatccgtga ggcgttcacg agccatcgca agatcgccat tctcaacttt
     4261 cggggttttc gggacttcta ccccgcgatg accgccgaca atctccgaaa gaattcgctt
     4321 ttcaaagaca tcgagatcat cgccggcacc atcacgctgc ccgccccgcg caccccgggg
     4381 aggaatccgt tcgagtttcg ctccatcgac attgcgcgta tcttcgattc cgaaaagtac
     4441 cttccgcgca tcgccgacga gatccgccgg gcggccgacg gcgcgcgcct gatcgggctt
     4501 ccggcgttca tcggcatcac ccgctacaac aagattcacg agcggctcga ggagctcacc
     4561 ggcgcgctca tctacgagat ccccaccctt ccgccgtcga ttcttggcat gcgcatcgat
     4621 acggcgttaa aaacccgttt tgcagcgctc ggcggcgtgt tcatatcggg cgatcgcgtc
     4681 aatggcggcg agatcgccga cggcgcgctc gaccacatcc acacggtgaa ccaccgcacc
     4741 atgcgtcatc acgcccgctg gtatgtcctc gcctcgggcc attttttcag cggcgggaac
     4801 gtgagcgagg tcaccggaat tcgagagccc atctgggggc tgtccgtccg cggcccggtg
     4861 gaccggagtg catggcgttc gcccgaattc ttcagcgcac acagccatcc gtttctggaa
     4921 tacggcgtga tcacggacga gtcgttccgt ccgatggacc cgggcggcgc gacggtccgg
     4981 aacctgtttt gcgtgggcgc agttcttgcg gactacaacc cgatccgcga ggggactggc
     5041 ggcggggtgg ccgtcgccag cgggtacgcc gccgcgcggc gcatcatcga ctcgatcacg
     5101 ggcgccgcgt catgatcaaa ctggaaaaca tcagtttcga ccactgtatc aaatgcacgg
     5161 tgtgcaccat ctattgcccc gtcgcgcgcg cgacccatct gttccccggc cccaagcagt
     5221 ccggcccgga caccgagcgg atgcggatca agagcccgga gctcgtggac gcgtcgctca
     5281 agtattgcac caactgcaag cgctgcgaga tcgtctgccc ctccgacgtg aagatcgccg
     5341 acatcatcca gcaggcgcgc tggaactatc tcaagaagaa gttccgcatc cgcgattttt
     5401 tccttagccg caccgacctg gttggcggca tgatgacccg gctcagcgcg atcgcgaact
     5461 tcttcatctc gcttttcccc gtaaagctga tgatgaccct ggtcctcaag atcccgtacc
     5521 agcgcgtgtt cccccgctac gagcagggga ccttcagaaa acgcttccgg tccatcgccg
     5581 cggcgcagcg ggcctttgat cgccgcgtgg tgtatttcca cggctgcttc gtgaactaca
     5641 acaaccatgc gctcggcttc acgctggtca gggtgctcaa cgcgttcggg atcggcgtgg
     5701 agatcgcgga cgaaaaatgc tgcggcgtcc ctttgatcgc caacggctac atcgagcagg
     5761 cgaagcggaa cggggcgtac aacatcgcgc atctctcgcg tgcgacgcgc gaccgcgagc
     5821 tcaccgttgt ctcgacctcc tcgagctgca ccttcgcgct caagtacgaa tactcccacc
     5881 tgctggagct cgacaattcg gatatctttc cgcgcatcga atacatcacc aaatacgtct
     5941 tttccctgct tgaggaggct gccgcgccgc cgctgaaacc cgtgggattg cgcgcggcct
     6001 accatgcccc ctgccacctt gagcgcatgg gcggggtccc gtatacgatc gaggtgctgc
     6061 gccgcatccc cgggctcgac ctggtgattc tccattcgga gtgctgcggc atcgccggca
     6121 cctacggatt caagagcgaa tattacgcca tttcgcagaa tatcggaagc gatttgttcg
     6181 cgatgatcga gcgcgccgag ccggaggtgg tcatcacgga ttgcgagacc tgcgccatgc
     6241 agatcgccat gaacacccca taccgcgttc tgcacccgct cgatgtgctg gcgatgtcgc
     6301 tcggggaatg acgctgcgca ctggagccga ccaatgaaat cccacaccga gtatctcacc
     6361 tttacgaccg catcacgccg cgactacctg aacatcaccc gaacgatcga gggaatcgtg
     6421 gccgcgagcg gcattcgtga gggcctgtgc ctcgtgaacg ccatgcacat caccgcgtcg
     6481 gtcttcatca acgacgacga gagcggattg ctccaggact tcgacgattt cctcgaacgg
     6541 ctcgcgccgc atgaaccgat cggccagtac cggcacaatc gcacaggcga ggacaacggc
     6601 gacgcccaca tcaaacgcac cgtaatgggc cgcgaggtcc tcgttgcggt cacgaacgga
     6661 cgcctcgact tcggcccctg ggagcagatc tactacggcg agttcgacgg gcgccgtccc
     6721 aaaaaggtgc tggtgaaggt gatcggggaa tagccgtgcg gctggtggac gtccactgcc
     6781 acctcgagga ccccgtcttc gacgaccggc tcgatgatat catcaccgat gcgcgccgcg
     6841 ctggtatcgc gcggatcatc accgcaagcg tcgttccctc ggatttcgcg aaatcccgct
     6901 ccatcgctgc gcgctatccc gaggtgtcct tcgcgctggg cattcacccg tggtacgtca
     6961 ccgaaacgag cgagcgcgat gccgattcgc tctccgcggc gcgcgatctc ggcgctgtcg
     7021 ccatcggcga gatcggcctg gaccggaaga tcgacgcacc agacttcgag ctccaacgcc
     7081 ggatattcga aaagcagctc gccatcgcga acgagctctc gttgccggtc gtcctccatt
     7141 gccgcggagc attttccgag cttgtcgaat cgctcaagag ggtcggccgc cccgttgggg
     7201 gcgggatcat acatgcgttt tcgggtagtg tcgagattgc cgaacggtgt attgcgatga
     7261 acctgtcgtt ttccatgggc gggacgctta cgtatcgaaa cagccgaaag cgggagacgg
     7321 tcgtgcggcg catttacccg gatcacttta tgctcgaaac cgacagtccc gacatcccgc
     7381 cggtggaatg ccgcgagaaa cccaatgtcc cgtcaaatat cctctacaac ctcgcggcgg
     7441 cggccgagac gcttggccga tcccgcgagg aggtggcgct tcggaccaca cagacggccg
     7501 agcgaatttt cggcctgccg ccatcctgac gcgcttcgca cgcacgcgtg cgtgcgaaca
     7561 cagcgtcgtg aatcgtgaaa aattaatggc ttgcaaaaat acatcacgcg tacatggtga
     7621 agtaaaatta tgatcgcgca gcgccatcag ccatgaacaa gatccttgcc gccatcatca
     7681 taaccatcgc gttcgggtgc gccgcgttcg cgcagaccgc ttcgatcccg ctcgtcgagg
     7741 aggaccgcga gttctggaac gacatccttc gcgacgagag catcaagaac aagaacctgc
     7801 tctatgcgag cttcgtcgcc tacaaggacc ggctcaacga cctttcgctg gagacctttc
     7861 gggagagcat gaagaacaat cagggcaatg atattgtcca gtgcctggcg cagtactata
     7921 tcgggaaaaa cctcatcgcc cttgggaagt tgcccgaggg gcttgcgcag ctccaaacgg
     7981 ccggatcgct gccctgcccg cgcgtggagc acttgaagta cgccatccgc atcaacaccg
     8041 cgatcgccca ccatcgcctg cgcaatttcc gcgagttcaa ccggatactc acggagatcg
     8101 tcaacgacga tagcgccgga cgctaccggt caatcgcccg gggcgtcctt acgcttgagc
     8161 gctagccgag gctagcgctt cggccgtccg atgcacgaat actcgaagcc gagctccttc
     8221 attttcgcgg gatcgtactg gttccgcccg tcgaatacga cctttttctt gagcagcgat
     8281 tccattttcg cgaagtcggg atgacggaac tggtgccact ccgtgagcag gaggagcgcg
     8341 tcggcgccct tcagggcgtc gtaataattg gacacatact cgacgccgtt tttgccgatg
     8401 atctcggacg cgatctccat ggcgaccggg tcgtgggcgc ggaatgtcgc ccccttctcc
     8461 cggagcgcgt gaatgatgtc gatcgcgggc gcttcacgca tatcgtcggt ttgcggcttg
     8521 aatgaaagcc cccacaccgc gaaggtgagc cctttcaccg tcccgccgta atacgcaagc
     8581 accttctgca cgaagagctc gcgctgctgc ttgttcacgt cttcgaccgc gtccaggatc
     8641 tgggaggtga ccccgaattc cttcgcggtt tgcacgatgg ccttcacgtc cttggggaag
     8701 cacgatccgc cgtagcccag acccgcgtag aggaacggca tcccgatccg cgaatcggac
     8761 ccgatcccct ggcgtatcat ctcgatgtcg gcgccggccg cctcgcaaag ccgagaaatc
     8821 tcgttcatga agctgatgcg cgtcgcgagc atggcgttcg cggcgtattt cgtgagctcc
     8881 gaagacagga tgtccatggt aaagatggga tgtccgtttc gaacgaaggg ggcgtagagc
     8941 tcgcgcatga gctccccggt gcgcacgttg tccacgccga cgacgatccg gtcgggacga
     9001 atgaagtcct cgatggcgtt gccctccttg aggaactccg ggttggacac gatgtcgaac
     9061 tcgacctcgc cgcggccgcg cttttcaagc tcctcgcgaa cggcggcgcg caccttctcg
     9121 ccggttccca ccgggacggt cgatttgttc acaacgatgg tgtacttgtt gatgagccgc
     9181 cccacctcgc gggcgaccgc aatcacatgc tgcaggtcgg ccgagccgtc ctcgtcgggc
     9241 ggggtgccga ccgcgatgaa cacgaaaagc gtctcatcga tgcactcctt gatgtcggtc
     9301 gtgaaatgca gccgcccctc gctgaagttg cgcgcgacca tctcctccag cccgggctca
     9361 tagatcggga tgatgccgtt cttcaggttt tcgatcttct tggcgtcgat atcgacgcac
     9421 cacacgtcgt ttcccatttc ggcgaaacag gttcccgtca ccaggcccac gtagccggtt
     9481 cccaccacag ttactttcac tggtccctcc ggatcgaaac aatcgttgtt cgtgttgatg
     9541 atgagggcgc gaccgcgtat tttatccggt acaatgcgag accggcaaac tcgcgcagcg
     9601 ccagcgagct cttgtaaaga ttatgcatgg tcggcagggc atcataggtg tcaagtttct
     9661 ttttcgaccg ggcgagctgg ccgcaggagt gcgggacgac ggacagaccc tcctcgcgga
     9721 agatgagcgt cgcccgccga agatgaaatg cagaggtcag caggatgctc gtacgaaagc
     9781 cgcgttcggc gatgatgggc cgcacgtatc gggcgttctc gaccgtgtcc cggcttctcg
     9841 attcgacgat gatccgctcc ggcggaacgc ccagatcgac gaggaaacgc cggtagacgg
     9901 gcgcctccgc cggccccgaa cccacgacgc caccgctcac gacgatcggc agcccatggc
     9961 gtcgatgcag ccgcacgcat tcgacgatgc gcaccatcat cacgggcgac ggtatatgct
    10021 cgccggtcag gtctgccgcc ccctcgacca gtccgccgcc aagtagaata atcgaatcgg
    10081 cgttatcgtg tgcggtagcg tggttcagga gttcgatccg gccaatcgcg aaatccgcga
    10141 ccgggccgat ggagaggatg tacaatgcgg ccgcggacgc catcagcaga gcgccgccgt
    10201 accggcgttt tcgcacagcg agtattccac ctgcgaacat gagcgcaatg agagcgccgg
    10261 gaatggtgat gatgcacagc agcaattttt tgattgtaaa cacctgcgtc ctccgccgcg
    10321 acgatggacc gcacattcct acgaggaact ggggggctca ggaagtggct ttgcgatgac
    10381 gttttaccgg gaccggcgtt gcgtcggcgc accgcccatt ttccacgtga aagtgccggt
    10441 ccgaatactg gatgatgttt ttatcgtgcg tggcgacgat aaacagaacg ccctgctcgc
    10501 gcgccaggtc ccgaaacagg gagaagatct ttgtgccgtt cgcctcgtcg aggttgccgg
    10561 tgggctcgtc ggcgagtatg acggacgggc ggttgatcat cgcgcgcgca atcgccaccc
    10621 gctgcttctc gccgcccgag agcgacgcgg gatactggcg gatgatcccc ccgagatcga
    10681 ggtactcgat gagatggcgc acccgctgcg agatatcgcg ggcggtcctg ttgatgagcg
    10741 cgggatagac gatgttctgg taggcgctga tatctgatat tagattgaac gattgaaaaa
    10801 cgtagccgat ctctcggttg cgaaggcggg aacgacgcag gtcgcccatc cggaaaatat
    10861 tacgcccctt gtaggtcacg gtcccgcggt ccggcttgag catgccggcg atgacgccca
    10921 ggagtgtcgt cttgccggac ccggacggcc ccgtgaggct cacgatggag ccctcgtcga
    10981 agacggcatc gacgccatcc agaatcaccc gtgcgccaaa acgcttgtga accgatgcaa
    11041 tctctattac gtgcatataa caaacccctc accatcgtga cggcgagggg tttattttca
    11101 attacttttt ctgcgatgtc agtacttggt cgattccttc aggaagtccg ccatgaggct
    11161 ttcggaatct ttcatgccct tgcgataggt ctcgccgatc atcagcaacg aattgtaaaa
    11221 gtaggtcaga tgcggaatca cgaaatcgcg cttgagctcg tttcggatgg gaaacgccgt
    11281 cgtgttctgg atctgatcca gggtcatgct tttcaggagg cgcttgttta cggaataatc
    11341 gaatttcatg cccttcttgt ccggtgtggc cgggctcgcg tcggtgacga gaacctgcgt
    11401 ttccgcctcg gtatcgtaat tccgctccca tacgatgaac tcaagcttgt tggccgattt
    11461 gccgtcggtg ccgatcttga gcttcttctt ctgtatgccg gttatgcgcg tattgtccac
    11521 attgcttctc gtgaagcgat gacgctctat ctcgatgaaa ccgtccccgc ggatgagtga
    11581 catttgatag gggagcgtct tgaatcggtt cgccttattc gccagctcgt aggttgcaat
    11641 cacctcggag atctttttgc tgaggtcggc gatctgcttg tcgagggccg tcccctcttc
    11701 cgcgagctcc ttggattcga gcgaatcctg atccgccttc ttctcctgcg ccgcggcggg
    11761 cgagaacgag agcgccatcg ccatgaccaa cagaataacc agcccgacta tgtatcgtgt
    11821 acgcatccgg tgcctccaga gggtaaatgt agacaatgat ttcaccacta ccttgactat
    11881 atcggcattc aggaataaaa aattaaacaa aataccgggc gccgccgacg gaacgtaatg
    11941 aagcctattc cggtcgcaat ggcatgtcaa acccttttcg aggcaaaata ttattgattt
    12001 tatgggcgat ctgattatat tgtaatagca atgaagcgcc tgatccacat cacgctcgcc
    12061 atcgccctcc tctcgtcggt gccgacgtgg ctgtccgcga aaaaggcccc atcggccgac
    12121 gacgagctca tgaaaaaggc catcgcctat ttctactcgc ggaagttcga gatgagcgag
    12181 ctcctcttcc aggaggcgct caagaaaaac cccgagaatg cgacggccta ctcgtatctg
    12241 ggcgatatct tcctgcagaa aaaacgctac gatggcgccc tgcagctcta caagcgcgcg
    12301 ctggacctca atccggaaaa cggcgacgaa tacttccggg tcgggcagat ctactattac
    12361 aagcggcagt cagaagacgc aatcgtcaat ttcaagcgcg cctacgagct caatcccaag
    12421 ctcaagttcg cgctctatca tatcgggctc acctacctga tgctcgcccg ggacaagcag
    12481 aacaccatcg tgaactggga gagctatctt gccgcggcgc ccgaagatcc ccagtacgag
    12541 aagctccgcc gcgccgtcga gctcctcaag gaccgcaact tcatcatccc caaggaagga
    12601 agcgacacct ccatcgagga ggcgcttcac ttgggcggca tggccctgca acgccagccc
    12661 cgcgcgatcg aggaccgcaa agcggaccac gaagacaaaa aaacaaaata taaacttgaa
    12721 gatattgagc gggacgacgg attatagtcc cgagccagag acggaggaac ccaccatgac
    12781 cctgtatcgc agcgacacct cacacgcggt aaaattcggc atcgttgccc ttgtcgccgc
    12841 cctgattatc ggcggaggca tttacctgtt ccaaagccgt gatcgcggcg tggacgaggt
    12901 gtccgatctc cggcttcgcc agatcgaact ctacaaaaag ctcctcgaca ccgagcccag
    12961 cagcattccc ctgctcatca aaatcgccga cctctacaag caggtcggca acctcgatgc
    13021 ggcgatcgac cattaccgga aggcgctggc catcgatccg atgaactacg acgccctgaa
    13081 cggcctgggg cacgcctatg ccctcaaggg agagtacgac aaggcgatcg agacgttcga
    13141 gcgggcgaag aaatcgggcc cacgcctccc ccgggcctac aatcgcctgg gcaatgtcta
    13201 tgatgagatc aacaaggagc agcaggcctt taccgaatac aaaaccgcga agcgggtggc
    13261 gccgaaggac caggagagct attacaatct cgcccgccag cgcctgaaaa agggcgacac
    13321 gcgcaatgcg atcaagtaca tcaaggaggg catgaaggcg aatccgcggg acgccgaggg
    13381 gcattacaac ctggggcgct tgtacctcga caccaagcag cctgatatgg cgcgccggag
    13441 cttcgcgaag gcgatctcca tcaacccccg cgtcgcaaag taccatcgcg acaagggcga
    13501 ggcggagctc cagctcggga agaactccga ggcggcctca tcgttcatta aaaccctcga
    13561 gatcgatccc acggacgcac gggcggctga gcgcctcggc gatcttgccg cgcgcgacgc
    13621 caactgggcc ggcgccgccg gctactacaa gaaggcgctt gagtacaatc acaacgacac
    13681 gaacctgcag cagaagtacc gaaacgcgct cgcgatgctc gaccggaaaa aagacatcgc
    13741 gaaaaaggtc gatccggaga cgcccgccgt tgcgaccgac gaccccgagc ggcccgtcat
    13801 caaaaaggat gatcgcgcca tggcgcccga aaccgacacc aaagacgcca tcgccaagga
    13861 cgattcggcg cgcaagaaac ccgacgccaa ggaaaacgac atcaagccct ccgcgcgcca
    13921 ataccgcgac ctgggagaca aataccgcgc ccagaaggac ttcgacaatg ccacaaaatc
    13981 ctacctgaag gccaccggga tcgacccgag cgacgaccac tcgcattact ggctcggacg
    14041 catctactat cacaacaaga tggacgatga ggcgctcaag tcgttccaga aggcgacggc
    14101 cgccaacccc aagaacgacg acgcccacta ccgcatgggg ctcatctact accagcagaa
    14161 gaagtacagc gcggcggtgc agtcattctc caaggccatc gccatccagc cgaattcccc
    14221 caaggccttc tatgcgcgcg gcctcgccaa ctacaagctg aacgacaaag gccaggcgat
    14281 caaggatttg aagaccgcca tctcccagga cccgcgcatg gatgtcgcct atttcaatct
    14341 gggctccatc tacatcgaca acaagaacta cgcggaggcc ctgaaatact tctcgaagga
    14401 ccgcgagctc cgtcccaaca acccggagac gcatttcaag atcggcgaga tctatcatga
    14461 gatgaaatcg ctcaacagcg ccgtccagtc gtaccgcaag accatcgagc ttgatccgac
    14521 ccattaccag gcgaacttca acctgggact ggtcctttcg cgccagaaga actatccggc
    14581 ggccataaag caatttgaac gggtgctggc caacaagccc gacgactatg cgagcatgta
    14641 cgagctgggg aaggcctacg aggggaccgg caacgacaat cgcgccatcg agtattattc
    14701 gaaggccatc gcgcgcaacc cagcctacag caaggcgtac atcaatctcg gggggctcta
    14761 tcggaaaaac aagttgttcg acaaggccat cgagcagttc agcaacgccg tatcgaacaa
    14821 tccgaaatcg ttcgaggcgc attacaatct ggccaacgcc tatcgcgaga ccaagatgta
    14881 cgatcagtcc atcgacgagt acaagatcgc gataaccctt cgcccgatga gctcgcaggc
    14941 ccatttcgcc ctcggcgagg cctaccgcga caagggcagc ttcgaggacg ccgcgcgcga
    15001 gttcgaactg gcgctccaat acaacaacaa gctcctggtc gcgcacgaag agctcggact
    15061 catctatcga aagatgaaaa acaccgacaa ggccgtctac cacttcgaac ggctcctgtc
    15121 ggcgcgcccg aaccaccccg atgccgacaa gatccaggag atgatcaatc tgatgaaaaa
    15181 atgaggacta gtccttgaca cggacgaccc gatcacgcgt aatcgcatca ctggcgccgt
    15241 gttgcgcgta ttccaagaca tccaaaccac gatgaaaaaa ttttccagta acatcttcgg
    15301 tcgatgcgcg ttaggcgccg gtgcgatcat cttgcttctg tgcgcgccga tgacctcgtg
    15361 catcattcgc gcgtacgatg ttgaccgcct gaaaagcgat tacaaaaccg agggttttct
    15421 ggacgccgac cactttcagg tcatcgtggt ggggacgccg gacaaggaca agcgcggcct
    15481 cgtacagaag cgcgaaagcg cgctcgagaa cgccaaaaac aagctcagca agaccgtcat
    15541 cgacaagtta agcgaatatt atctcagcgt ccacctcccg accctcggga tcggcagcgc
    15601 caaggagatc gccaatttcg acgatgtgcg cgtcaatctc acccacgagc tcatgccctt
    15661 cctcaacgac ggctccgtcg cgttcgagta ctacaactac gatcattccg cggtcattgt
    15721 atttcgggtc atacggcgaa accttcagcg cgcgctcgcg tccgttgttc tgcccgtcgc
    15781 catacagaag aagtaaagga gaacgctcat gaaatttaac gccattacca ttccggccgg
    15841 acccgcaccg agagcgctgt gcgcgattgc gatcgccgcg tccctggcgc tatcgcactg
    15901 ctctacgaaa tcgacgagcg ccgtcacaaa gcccacacac ggagggaatg cgaacctcag
    15961 cgatcgcgac gtcctcccgg ccgagtacca gcgcgagggc ttcatcaaca aggacctcta
    16021 ccgcatcgta atcgtatcgc ccgccgacga gtgtccggac atgagctatg tcgaaaaaaa
    16081 tgcgcgccaa cgcgccttca tgtcgctgca gaagcatttg gagtcacgca ataaaatcgt
    16141 ggaccagaac acgcgcgcgc gcctgctcaa cctcatcaac gagaaaggaa cggtaaaaaa
    16201 cctggagtgc cgcgacgaaa agaggaaggc gttcgtgttc gagatcgaga tgaaaaacct
    16261 aggccagtac ctggacagcc tcgcgggcca gcgctagcgc ccgtccgccg ggcccgtttc
    16321 cgcatcttgc gacgcatgca tgcgataggc gagatgcttg aagataaaaa gctccaggct
    16381 ttccctgttg tcgtcgtaga tgctgctgaa catgaccccg atgccgaagc gcccgcgccc
    16441 cttcggctcc gaacgcatca ccatggccag cgcctcgatg ctgcccaacc ccggatcgaa
    16501 acccgaaaag tcgatggtga ccctgagaat gctcgatacc ggcaccgcgc gaggagcgaa
    16561 aaagtaaaat ccgcccctgc tgatattgac gatggtcc
//
LOCUS       DAORHF010000053        16585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2574_length_16585_cov_6.174592, whole genome shotgun sequence.
ACCESSION   DAORHF010000053 DAORHF010000000
VERSION     DAORHF010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16585
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2574_length_16585_cov_6.174592"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..652)
                     /locus_tag="PKJ16_06535"
     CDS             complement(197..652)
                     /locus_tag="PKJ16_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNV46681.1"
                     /translation="MKRCISASSIVFLLLLGSSESFSQQGRGQGLRGRTYNPATVETI
                     SGRIDEVVFVKGGRGRSAQGVHLALSTTGGSIEAHLGPASFVDGKMTFAKGDTVMIVG
                     SRMTMGGRDAIIAKTVTKGGTVLTLRNDDGTPLWAGFYGPGRGRGRQGR"
     gene            923..1381
                     /locus_tag="PKJ16_06540"
     CDS             923..1381
                     /locus_tag="PKJ16_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HNV46682.1"
                     /translation="MMGRGRGAWWQDRFADRGARITAPREVVLEILQETDDHVTAADV
                     YVKAHERNPSIGLTTVYRTLDMLTQMGIVQKFDFGDGKARYELVNNPGGKKHHHHLVC
                     LRCKAIVDYTEFLDDELEFIGKTQQRLSKKYDFDITDHAISFYGVCARCR"
     gene            1445..1834
                     /locus_tag="PKJ16_06545"
     CDS             1445..1834
                     /locus_tag="PKJ16_06545"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HNV46683.1"
                     /translation="MPGGDRTGPLGHGSKTGRGAGFCAGYGVAGFQNVGAGFGYGRGR
                     GFGGGGYGGGGRGWRHVFYATGLPRWARGAYGNQGWGGAVGYGEFTPEREMELLKTQS
                     ELFGKQIDVLNARIKELEELAAKKGDA"
     gene            1940..2377
                     /locus_tag="PKJ16_06550"
     CDS             1940..2377
                     /locus_tag="PKJ16_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV46684.1"
                     /translation="MTISVDANKCSGCGACVGECPNAAITIEEIAVIDAVRCTECGVC
                     IDACPVGALSMRVSKNRLGRKEALVGGGQGRSGRGSGGGDGMGQGRGQGRGRGRCGGM
                     SGANTVDAGQCACPACGATMQHRRGTPCTQLRCPKCGAAMVRQ"
     gene            2416..2613
                     /locus_tag="PKJ16_06555"
                     /pseudo
     CDS             2416..2613
                     /locus_tag="PKJ16_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            2999..3382
                     /locus_tag="PKJ16_06560"
     CDS             2999..3382
                     /locus_tag="PKJ16_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HNV46685.1"
                     /translation="MDQSDAERRATAFPDIEGTQGRMNDPDGAAIIKGICGDTMEMYL
                     AISGGRIEDACYATDGCGSSHACGSAAAGLARGRELREALRISPAEVLDALGGDPAVE
                     VHCAILAVMTLHKALAEYIIRFEES"
     gene            3413..4045
                     /locus_tag="PKJ16_06565"
     CDS             3413..4045
                     /locus_tag="PKJ16_06565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV46686.1"
                     /translation="MEELHESFTTREIAENFARAEERSALFRRHGVDQDALRSSIVRQ
                     LEGAPAPVLEVGTGKGRLTVAIARTVGDVVSVDTDPDGMRMAALLAAYYGVRERIHFL
                     LSDAARLAYPDGHFDTAVSAFTLHHLERPEAVFDEMARVTGRLVLISDFNEAGFAALD
                     RIHESEGRAHDRGSGAFGIRDAGDQLVRLGFRVRMVEEELQTMCVATRGQ"
     gene            4042..4902
                     /locus_tag="PKJ16_06570"
     CDS             4042..4902
                     /locus_tag="PKJ16_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HNV46687.1"
                     /translation="MRSSLECLPCLVRQAVESARRVIADDTACEEAMRGIMAHVASMD
                     YALPPPVLAQSMHRVIRAATGGADPYRAEKARFNTLARSLLPSFQARIARAADPFDAA
                     VRVAIAGNVIDFGAKGDLSENEAVAAMDRAFEAPVDGDIRALREAAQRASRILFLADN
                     AGEIVFDTPLAGMLPAGSVTVAVRGFPVINDATHEDAEAAGMSALARVIDNGSDAPGT
                     ILADCSDAFRTAFASADLVIAKGQGNYETLCDEPGNIFFLFKVKCAVVARHSGFGVGT
                     HAVVRARSRA"
     gene            complement(4940..5176)
                     /locus_tag="PKJ16_06575"
     CDS             complement(4940..5176)
                     /locus_tag="PKJ16_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV46688.1"
                     /translation="MPTYEYACESCDHRFERFHGMNDEANITCPRCGGAAKRLISGGA
                     GIIVKGGTGRSTACGSDVPCCGRGEPCGKSHGCS"
     gene            complement(5191..5478)
                     /locus_tag="PKJ16_06580"
     CDS             complement(5191..5478)
                     /locus_tag="PKJ16_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46689.1"
                     /translation="MDGAEILLRTGGGHCIEWAAKREYRTLVDRLIANAEREDPDAEA
                     RLALLLEFLEQADFAALRSSDEYLSGEKEATCLLLRDERGVPRVYVLRDAS"
     gene            complement(5482..5820)
                     /locus_tag="PKJ16_06585"
     CDS             complement(5482..5820)
                     /locus_tag="PKJ16_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV46690.1"
                     /translation="MKIAMPVSNGKLCMHFGHCEQFDVYNVDDATKLVSVLERLDAPP
                     HEPGLLPRWLAGKGVTCVIAGGMGSRAQSLFKEQGVAVVTGAPAGDPEEILAAYLSGR
                     LVTGANACDH"
     gene            complement(5817..6704)
                     /locus_tag="PKJ16_06590"
     CDS             complement(5817..6704)
                     /locus_tag="PKJ16_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008605093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HNV46691.1"
                     /translation="MFQKKDKQPTACASGASPSVNASRDADDEKALKENLSRITRKIV
                     VLSGKGGVGKSTVAVNLAVSLAARKLRTGLLDIDIHGPSVPTLLGLQGAKIAVTGENK
                     IHPVEYNEYLRVMSVGFLLSDHNDAVIWRGPLKYGIIKQFLKDVEWGDLDYLIVDSPP
                     GTGDEPLSVCQLIDRAEGAVIVTTPQEVALIDVRKSITFCRQLNMPVLGVVENMSGFV
                     CPHCGKESPIFKNGGGKAMADDMGVPFLGAIPIEPGIVQNGDAGTPFHNAAVQNATPA
                     QRAFSSIIDALNPTKGERS"
     gene            complement(6752..7279)
                     /gene="tsaA"
                     /locus_tag="PKJ16_06595"
     CDS             complement(6752..7279)
                     /gene="tsaA"
                     /locus_tag="PKJ16_06595"
                     /inference="COORDINATES: protein motif:HMM:NF014082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HNV46692.1"
                     /translation="MPSNEYALRPIGRIRTPHATVSDNVPIQGRMAPLSEGAVELFPE
                     YAAGACDLEGFSHVFLLYRFHLGTDERLTVVPYMDTVPRGVFSTRFPHRPNRIGVTLV
                     EVLGVDGTTVRVRGVDMVDGTPLLDIKPYVPRFDAVENVASVRTGWMTPYVNGVKEPD
                     VVRTSSETDWLRERR"
     gene            complement(7266..8126)
                     /locus_tag="PKJ16_06600"
     CDS             complement(7266..8126)
                     /locus_tag="PKJ16_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46693.1"
                     /translation="MKELVIISGKGGTGKTSVTASFAALAENAVIADCDVDAADLKLV
                     LGGTTVERERFVGGKKAFIDPARCDRCGTCAPLCRFDAISASCAIDPLACEGCGVCAH
                     FCPCGAIEMREHVSGERMRTETPHGALAHAKLGIAEGNSGKLVTLLRKKAREYAEGAG
                     ANLIIADGSPGTGCPVIATITGASYVLIVTEPTVSGMHDLTRVNELARHFRVPSAVCV
                     NKADINEETTREIRDYCAAAGIPMLTPIPYDPDVTKAQIAGLSVVECSAGPAATAIRR
                     LWEEVSRAIE"
     gene            complement(8122..8802)
                     /locus_tag="PKJ16_06605"
     CDS             complement(8122..8802)
                     /locus_tag="PKJ16_06605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV46694.1"
                     /translation="MRDELRSIGDACRAALNEYTVRAFSLLPPIDGMRILDAGCGSGE
                     PALALARMSAARICAIDPDAASCAAMGRKVARDGFANRMTVMNVDFASPEIQKNPFDV
                     VWAEGLFNIIGFEAGLARAGELLKPGGYLVIHDEISRGDEKKRLWEKHGYGIVDSFVL
                     PENAWPDYYKCMKTALAALGDSGRARGDDSLVRAAEDELAAIEEQMKRPENLRSVFYI
                     LRKRDAHD"
     gene            complement(8792..9661)
                     /locus_tag="PKJ16_06610"
     CDS             complement(8792..9661)
                     /locus_tag="PKJ16_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46695.1"
                     /translation="MNIAVASGKGGTGKTTIALSLARYIAHNGSRVAVLDCDVEEPNV
                     NLFLGAAIERTERAGVPVPSVDPAACTGCGACERICAFSAIVLINERPLVLPDMCHSC
                     GGCALVCPTAAITEVPKEIGDIEYGSRNGIDYAGGRLAIGGHLSPPLINQVKRAYGSY
                     DVRILDCPPGTSCPVIEAARGADYLVLVTEPTPFGLNDLMLAVDMVRAMGLPFGVVIN
                     RDGMGNADLERWCGVNDVRIVARVPFDRRIAEEYSRGDCASRIIECYGEQIALVAAAI
                     APAANHDRGTHAG"
     gene            complement(9677..10441)
                     /locus_tag="PKJ16_06615"
     CDS             complement(9677..10441)
                     /locus_tag="PKJ16_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46696.1"
                     /translation="MLIERDGVSLFDTGQGLGLMHNVSVLGKNPAAVDRVILSHGHYD
                     HTGGLLAFAQGRDAALPVFVNPDAFIPKFAVYGAANAQSALPIGFPRERGEYERAGCD
                     FHHTTGHEAIEDGMHALSAIPRPEGWRGFDAMLKRKEGDAYVDDPFTDDLSLCVETDS
                     GPVVLLGCAHAGIVEILDAISRETGFGSFHAVIGGTHLGSAPVEYVYRTIDALQRYRV
                     RRVGVSHCTGSRAALMITSAFPEAFTPASVGAVFEF"
     gene            complement(10521..10880)
                     /locus_tag="PKJ16_06620"
     CDS             complement(10521..10880)
                     /locus_tag="PKJ16_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV46697.1"
                     /translation="MKIAITSTGTDTQSLVDERFGRAKGFIVHDMESGASEYIDNAQN
                     MDSPQGAGIQAAKTVINAGVSALITGHVGPKAHAALIAAHVDMFTGASGTVDQAIQDF
                     QDGKLTQASQATVEGHW"
     gene            10985..11395
                     /locus_tag="PKJ16_06625"
     CDS             10985..11395
                     /locus_tag="PKJ16_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HNV46698.1"
                     /translation="MSRPKCCRKVGIVPTTTIYRPEGDMPFAGGEIVLSLDEFEAIRL
                     ADLSGLYQEDAAKKMDISRQTFGRIVESAHRKIADALVNGKSLRIEGGAVSVETPPRC
                     RCDKCKRSLDCPHRFREDGCPRIGHGKESINPGQ"
     gene            11412..11774
                     /locus_tag="PKJ16_06630"
     CDS             11412..11774
                     /locus_tag="PKJ16_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HNV46699.1"
                     /translation="MKIAVPSNQNQVDAHFGHCEYFTVFTANDKNEIVSEEKVMSPAG
                     CGCKTNIVGTLSQMGVKVMLAGNMGDGAVNVLNSHGIKVVRGCAGTVKSVAERFLAGA
                     VSDSGIGCHEHEHGCHES"
     gene            11780..12040
                     /locus_tag="PKJ16_06635"
     CDS             11780..12040
                     /locus_tag="PKJ16_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV46700.1"
                     /translation="MKTVTQLPIIDFANCVKCMNCVTACPRSAIVEPINTSCAKCVKY
                     CISMEVPCSPENIIFDYALCDSCGECLSACPEGAIYWFTPSA"
     gene            complement(12149..12895)
                     /locus_tag="PKJ16_06640"
     CDS             complement(12149..12895)
                     /locus_tag="PKJ16_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007547450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HNV46701.1"
                     /translation="MSDILITAFALSVVLVGIHAFFGTEIIRRGIIFTDLAIGQMAAL
                     GAAASLFFIDGQFVYPISLGFALAGGLLIAWASRHTDNLEAFIGLMYAFGISAVYIIL
                     SKSAHGMEEFQRLMASDILFTPQEEVVKTAIIYAVLGVVLFALYRRIDGFWKDALFFV
                     SFAVTVTSSVKLAGVLIVFALLVAPALIAIRVGGGRPLVVAWIVGTLVNLVAIALAHR
                     FDFPTGYTIVFCNALLALLVTIIPRRAKRG"
     gene            complement(12892..13794)
                     /locus_tag="PKJ16_06645"
     CDS             complement(12892..13794)
                     /locus_tag="PKJ16_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HNV46702.1"
                     /translation="MKRTVLIPLAVAMIASVAAPLRAERLKVVTTYPYIASIVEQIAK
                     DTVRVDALARGDYDPHTIVPKPSFIAKLRKAHLLIINGAQLEIGWLPPLLKQANNASV
                     NPGTRGFLDLSGHVTLIDVPKSVSRAQGDVHPDGNPHYYIDPHVIPRLARAICTKLSE
                     VDSRNAAMYKKNYDEFAARWKAKRASWDAKLRRLKGAKVIQYHKNMDYLLIRYGLVRM
                     GTVEPLPGIPPTSKHIAELEKIFESNTIRFILQDVYNADDSSRHLSKKFGVKMVKVPH
                     DVGAVREAEGIFSLFDEMVRRLTQ"
     gene            complement(13811..15310)
                     /locus_tag="PKJ16_06650"
     CDS             complement(13811..15310)
                     /locus_tag="PKJ16_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46703.1"
                     /translation="MKRFTVILLVFIFSVAIPSLRSQDRPDAPKEPAQKELRDDSPAD
                     LPNYDDDDATLEPRNESKDPGNGSPDEPENYDDEERGAARTAPKRDAQSMKEGTTSSP
                     FSQSQFVPDISFVLDASYNHRDRDNEIYKTQLRPGFIQSMSGEAEHEHVAPNAVMRGF
                     NFNYAELTLYSVVDPYFDLFAAIELTTAKVLIEEAYFCTTSLPFGFQLKTGKFLSSFG
                     RINEQHAHNWDFYDAPLVYEAFFGGDLNEVGVRATWVAPLDFYLMIGAEALQGANAAS
                     FGATGFADPNEIMRVRDSDDPNLGVGYIKTSFDIGDLTVYMGASGAYGRSRINHGVDQ
                     AGVAGTAIAAHSHVLGADLTLKYQFDSKRYCALQTEYLYRFMDGAYYAKNETDIVEEY
                     ALVKRQSGLYAQLVGKFAMRWRAGIRYDLLAVNDIKLDGIKESLPESLPRYSAMVDFN
                     ATEFSRFRLQYNYDHSMYVPRDGSFASRPNHEVVLQVNITIGAHGAHAF"
     gene            complement(15444..15725)
                     /locus_tag="PKJ16_06655"
     CDS             complement(15444..15725)
                     /locus_tag="PKJ16_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46704.1"
                     /translation="MKKLAYIALLLAAVLAIDIAMVAFHHHENGVIIDDCPLCRFQDS
                     GFTAKAQGSACVLSTPDFVHVDVFIVYESVSLHNHALLASRPNAPPFTA"
     gene            complement(15852..>16585)
                     /locus_tag="PKJ16_06660"
     CDS             complement(15852..>16585)
                     /locus_tag="PKJ16_06660"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HNV46705.1"
                     /translation="GWKLHHIREQASVLSEKLAALGADVVEFPTIRIARRGDLSDLRA
                     ALADIGSYRWIVFTSQNAVSIFFDELAAAGMDARALAGARIAAIGPATANELRVRSIN
                     PDLVPAEYVAEAVIDAMKTVGVAGSRVLLPCATEARAALADGLRSLGATVERIHLYDT
                     VVPDTIDPDALADVRDADMVTFASSSTVRNFFAIVKESRAQFACIGPITADALKELGH
                     DPAVVAPVYTIDGLVDAISGHYEKY"
BASE COUNT         3133 a         5198 c         5210 g         3044 t
ORIGIN      
        1 atacccgatg tctggtgggt agtgcaaagt attggggtca catcatatca ggggaaaggg
       61 gggcgaagaa aattaccatt ggcgcacctc cccctaacaa agggggaggt tgggaggggg
      121 ttggtgcaat cagtgctgaa taaaaaaatt taaaattttg tgcaatgttc gcaggagaac
      181 agtatagata gatattctat cgtccctgcc gacccctgcc tcgtccggga ccgtagaagc
      241 ccgcccagag cggcgtgccg tcgtcgtttc gaagcgtgag aacggtcccg cccttcgtaa
      301 ccgttttcgc gataatcgcg tcccgtccgc ccatcgtcat gcgcgatccc acaatcatga
      361 ccgtgtcgcc tttcgcgaac gtcatcttgc cgtcgacgaa cgacgccgga ccgaggtgcg
      421 cttcgatgct cccgccggtg gtcgataacg cgagatggac gccctgcgcg ctcctccccc
      481 tgccgccctt cacaaacacg acctcgtcga tccgtcccga gatcgtttcg acggtcgcgg
      541 gattataagt gcgcccgcgc aatccctggc cgcgtccctg ctgcgagaag gattcggatg
      601 aaccaaggag cagcaaaaag acgattgacg atgccgatat acagcgtttc atgttaccct
      661 cccggggttg ctgatggatt ccgttctggt gtccggacgg tgctgcatgc ttacacagat
      721 gcggcggtgc cggatcagcg tatttgatag tgaaaactat tatcataaag atggatgcct
      781 gtcaagaaaa acacgccata attcgtcggt gaacgttttt ttaagaaaat gacagaaata
      841 ttgaaaaacc attgacgatc cgacggggat tctatatatt gataatgaaa atcgttttca
      901 ttacaaggaa ggatggcgtc gtatgatggg tcgaggacga ggcgcctggt ggcaggacag
      961 gtttgcggac cggggcgcgc gcatcaccgc gccgcgtgag gtggtgctcg agatcctgca
     1021 ggagaccgac gatcatgtga cggccgcgga tgtgtatgta aaggcgcacg agcggaatcc
     1081 ctcgatcggc cttacgacgg tgtatcgcac gctggacatg ctcacgcaga tggggatcgt
     1141 gcaaaagttc gactttggcg acggcaaggc gcgctatgag ctggtgaaca atcccggcgg
     1201 gaagaagcac catcaccatc tcgtgtgcct gcgctgcaag gcgatcgtcg attacaccga
     1261 gttcctcgac gatgagctcg agttcatcgg caagacgcag cagcggctgt cgaagaaata
     1321 cgatttcgac atcaccgacc acgccatcag cttctacggg gtgtgtgcgc ggtgtcggtg
     1381 aggaaatttt ttttgagcat ttaatgataa tggttttcat ttttaattct aaaggaggca
     1441 ggtcatgcca ggtggagaca gaacgggacc gttgggacat gggtccaaga cgggacgggg
     1501 cgcgggtttt tgcgccggat acggagtcgc gggatttcag aacgtcggcg ccggattcgg
     1561 gtatggccgc ggcaggggct tcgggggcgg cggctatggc ggcggaggcc gcggatggcg
     1621 gcatgtgttt tacgccaccg gactgccccg ctgggcgcgg ggcgcctatg gcaatcaggg
     1681 ctggggcggc gcagtcggct acggcgagtt cacgccggag cgcgagatgg agcttttgaa
     1741 aacgcaatcg gagctgttcg gcaagcagat tgacgttctc aatgcgcgca tcaaggagct
     1801 ggaggagctc gccgcgaaaa agggcgatgc gtgatcacag tactgcgata ctgccggttc
     1861 tcgtcgccgg cggtatcgct tttcgcgaac ggccacgaag gctgctggtg atggaatctt
     1921 atcgaggaga aatcagatca tgaccatttc ggtcgatgcg aacaagtgca gcggatgcgg
     1981 ggcctgtgtc ggggagtgtc ccaatgcggc gatcaccatc gaggaaatcg ccgtcattga
     2041 cgcggtccgg tgcaccgagt gcggcgtctg cattgacgca tgtccggtgg gcgcgctctc
     2101 gatgcgcgtt tcgaaaaatc gacttggacg gaaggaggcg cttgtgggcg gcggacaggg
     2161 aagatccgga cgggggtccg gcggtggcga cggcatggga cagggtcgcg gacagggccg
     2221 aggacgaggg cgatgcggcg gcatgagcgg cgcgaacacc gtcgatgcgg gccaatgcgc
     2281 gtgtcccgcc tgcggcgcga cgatgcagca ccggcgcgga acgccctgca cgcagctgcg
     2341 ttgcccgaaa tgcggcgccg ccatggtgcg gcaatagtaa tgcgcgccga tgaatgcttc
     2401 acaaggtgac cagccatgat tgaagattat tacggggttc tcggtatcga tgccaccgcc
     2461 tccgaggacg agatcaaggc tgcctttcgc aggcttgccg tacggtttca tcccgacaaa
     2521 aatcccgacg atgccgatgc cgagcggcgc ttcaaggaga tcgcggcggc ctacgatgtg
     2581 ctctccgatc cctatgcgcg aagcgcctac aatctgtcgc gctccggcgg cgcgtatacc
     2641 ggttccggtt tcgccactgc cggcgatacg cacacggacg cggcttgcgg ttggggacgc
     2701 gggtgctgcg ggaaacgcgg cgcattcgcc cgccgcttct tctcctccgc atgcgtggtg
     2761 aagctcacgc cggaggaggc gcgtcgcggc gcggagcgga cattcgtttt ccgcgacggc
     2821 agcggctatc atcgggtgtc ggtggccata ccgccgaacg cgatcgacgg cgccgtgttc
     2881 cacgtcaatc cgcccgacgc tgattctcac agcgaaggat ttgacattca catcgccgtg
     2941 cggtagatga cgccatgccc cccgcggggg gatcacccgg ccgtgaggag gtgacgccat
     3001 ggaccagtcc gatgccgaac gccgagcaac ggcatttccc gacatcgagg gaacgcaggg
     3061 ccgcatgaac gatccggacg gcgccgcgat catcaagggt atctgcggcg atacgatgga
     3121 gatgtatctc gccatttccg gcggccgcat cgaggatgcg tgctatgcca ccgacggctg
     3181 cggttcgtca cacgcctgcg gctccgcggc ggcgggcctg gcgcgcgggc gggagcttcg
     3241 cgaggcgctc aggatctctc ccgcggaggt gctggacgcc cttggcggcg atcccgccgt
     3301 cgaggtgcat tgcgccattc tcgcggtgat gacgctccac aaggcgctgg ccgagtatat
     3361 catcaggttc gaggagagct agcgcggccc tgcggcggcg cggagaaggc agatggaaga
     3421 attgcacgaa tcctttacca cccgggagat cgccgagaat ttcgcgcgcg ccgaggagcg
     3481 ctcggcgctg ttccggcgcc atggcgtcga tcaggacgcg ctgcgttcgt ccatcgtgcg
     3541 tcaactcgag ggcgcccctg cgccggtgct cgaggtgggc accgggaagg gacgtctcac
     3601 ggtggcaatt gcgcgcaccg tcggcgatgt ggtgagcgtc gataccgatc ccgacggaat
     3661 gcgcatggcc gcgctcctcg ccgcgtatta cggggtccgc gagcgcattc atttcctttt
     3721 atccgatgcc gcccgattgg cctatcccga cggccatttc gacacggcgg tctcggcgtt
     3781 tacgctgcat catctggagc gccctgaagc ggtgttcgac gagatggccc gcgtaaccgg
     3841 gcggctcgtc ctgataagcg acttcaacga agcgggtttc gcggcactgg accggataca
     3901 cgaaagcgag gggcgcgctc acgaccgcgg gagcggggct ttcgggattc gcgatgcggg
     3961 cgaccagctc gttcggctcg gctttcgcgt gcgaatggtc gaggaggagc tgcagaccat
     4021 gtgcgtggcg acacgcggcc aatgagatcg agcctggagt gccttccctg cctggtgcgc
     4081 caggccgtcg agtcggcgcg ccgcgtgatc gcggacgata ccgcctgcga ggaggcgatg
     4141 cgcggaatca tggcgcatgt cgcgtcgatg gactacgcgc tgcccccgcc ggtcctggcc
     4201 cagtcaatgc atcgcgtaat ccgcgcggcg accgggggcg ccgaccccta tcgcgcggag
     4261 aaggcgcgct ttaacacgct ggcgcgctcg ctcctgccgt cttttcaggc gcgcatcgcc
     4321 cgggccgcgg acccattcga cgccgccgtc cgggttgcga ttgcgggcaa cgtgatcgac
     4381 ttcggggcca agggagacct ctccgagaac gaggcggtcg cggcgatgga ccgcgccttc
     4441 gaggccccgg tcgacggcga catccgcgcg ctgcgcgagg ccgcccaacg cgcgtcgcgc
     4501 atcctgttcc tcgccgacaa tgccggcgag atcgtcttcg atacgccgct ggcgggcatg
     4561 ctccccgccg ggagtgtcac ggtggcggtg cgcgggtttc cggtcatcaa cgacgccacc
     4621 cacgaggacg cggaagcggc gggcatgtcc gcactcgcgc gcgtgattga caacggatcg
     4681 gacgcgccgg gaaccattct ggccgattgc agcgacgcct tccgcacggc gtttgcgagc
     4741 gccgacctcg tgatcgccaa agggcaggga aactacgaga cgctctgcga cgagccggga
     4801 aacatcttct tcctatttaa agtcaagtgc gccgtggtcg cgcggcacag cgggttcggt
     4861 gttggaacgc acgcggtggt tcgggcccgg tcacgggcgt aaccggcatt tgcgcacgtc
     4921 ggggcagcga catcgtacgc tacgaacacc cgtgcgactt cccgcacggc tcgccccgcc
     4981 cgcagcaggg aacatcactc ccacaggcgg tcgagcggcc cgtcccgccc ttgacgatga
     5041 tcccggcgcc gccggagatc agccgcttcg ccgcgccgcc acatcgggga cacgttatgt
     5101 tcgcttcatc gttcatgcca tgaaaccgtt cgaagcgatg atcgcacgat tcgcacgcgt
     5161 attcataggt cggcatggtt cacctcgttt tcatgaagca tctcgtagta cgtacacgcg
     5221 cggcactccc cgttcgtcgc gcaggagcag gcaggtcgct tctttttccc ccgacaggta
     5281 ttcatctgac gagcgcaatg ccgcgaaatc ggcctgctcg agaaactcga gcaggagcgc
     5341 aagccgggcc tcggcgtcag ggtcctcccg ctcggcattc gcgatgagcc gatcgacgag
     5401 cgtccggtac tcccgcttcg cggcccactc gatgcagtga ccgccgcccg tcctgaggag
     5461 tatctccgct ccgtccatca atcagtgatc gcaggcgttg gccccggtga ccagccgccc
     5521 cgacagatac gccgcgagga tttcctccgg atcgcccgcc ggcgcgccgg tgacgaccgc
     5581 aacgccctgc tccttgaaca gcgactgggc gcgcgatccc atgccgccgg cgatgacgca
     5641 ggtaacgccc ttccccgcca gccagcgggg aagaagcccc ggctcgtggg gcggagcgtc
     5701 gagccgctcg aggacgctca ccagcttggt cgcgtcatcg acgttgtaca cgtcgaactg
     5761 ctcgcagtgg ccgaagtgca tgcacagctt tccgtttgat accggcatcg cgattttcat
     5821 gatcgttctc ctttcgttgg attgagcgcg tctatgatgc tcgaaaaggc ccgctgcgcg
     5881 ggggtcgcgt tttgcaccgc ggcattgtga aacggcgttc cggcgtcccc gttttgcacg
     5941 atacccggct cgatcgggat cgcacccagg aacggcacgc ccatatcgtc cgccatggcc
     6001 tttcccccgc cgttcttgaa gatgggcgac tccttcccgc agtggggaca gacgaagccg
     6061 ctcatgttct ccacgacgcc cagcaccggc atattgagtt ggcggcagaa ggtgatggat
     6121 ttgcgcacgt cgatgagcgc cacctcctgc ggcgtggtga cgatgacggc cccctccgcc
     6181 cggtcgatga gctggcacac cgacaggggc tcgtctcccg tgccgggcgg cgaatcgacg
     6241 atcagatagt ccaggtcccc ccactcgacg tccttcagga actgctttat gatcccgtac
     6301 ttgagcgggc cccgccagat gacggcatcg ttatggtcgg acagcaaaaa cccgacggac
     6361 atgaccctga ggtattcatt gtattccacg ggatgaattt tgttttcgcc ggtcacggcg
     6421 atcttcgcgc cctgcagtcc caggagcgtg ggcacgctgg ggccgtgtat gtcgatgtcg
     6481 agcaggccgg tccgcagctt gcgcgcggcg agggagaccg cgaggtttac cgcaaccgtg
     6541 ctcttcccca cgcccccctt tccggaaagc acgacgatct tgcgggttat gcgcgacagg
     6601 ttctccttca gcgccttctc gtcatccgcg tcccgactcg cgttcacaga cggcgaggcg
     6661 cccgaggcgc atgcggtcgg ctgtttgtcc tttttttgaa acatggtcat tctccacaga
     6721 tagatagtat ccgccgccgg acaatgccgg tctagcggcg ctcccgcagc cagtccgtct
     6781 cgctgctggt tcgcacgaca tccggttcct tcacgccgtt cacgtagggc gtcatccagc
     6841 ccgttcgcac ggatgcgacg ttttcgacgg cgtcgaaccg gggaacatag ggcttgatgt
     6901 ccaggagcgg cgtgccgtcg accatgtcga cgccccgcac gcgcacggtc gtcccgtcga
     6961 cgccgagcac ctccaccagc gtgacgccga tacggttcgg ccgatggggg aagcgggtcg
     7021 aaaagacgcc gcgcgggacg gtgtccatgt acgggaccac cgtcagccgc tcgtccgtcc
     7081 ccaggtggaa ccggtacagg aggaatacgt gggaaaatcc ctcgaggtcg cacgcgcccg
     7141 ccgcgtactc ggggaacagc tccaccgcgc cctcgctcag cggggccatc cgcccctgaa
     7201 tgggaacgtt atccgaaacc gtcgcgtgcg gcgtcctgat gcggccgatc ggccgcaggg
     7261 catactcatt cgatggcacg ggacacctcc tcccagagcc ggcggatagc ggtcgcggcg
     7321 ggacccgccg aacattcgac cacgctgaga ccggcaatct gcgccttcgt gacgtcggga
     7381 tcgtagggga tcggcgtcag catcggaatg ccggcggcgg cgcagtagtc ccgaatctcc
     7441 cgcgtcgttt cctcgttgat gtcggccttg ttgacgcaga ccgccgacgg cacccggaag
     7501 tgccgggcga gctcgttgac gcgcgtgagg tcgtgcattc ccgaaacggt cggctcggta
     7561 acgatgagca catagctggc cccggtgatg gtggcaatga ccggacatcc cgttccgggc
     7621 gaaccgtcag caatgataag gttcgccccg gcgccttcgg cgtattcgcg cgcctttttc
     7681 cggagcaggg tcaccagctt tcccgagttc ccctcggcga tgccgagctt cgcgtgcgcc
     7741 agcgcgccgt gcggggtctc cgttcgcatc cgctcgcccg atacgtgctc ccgcatctcg
     7801 atcgcgccgc acgggcagaa atgggcgcat acgccgcaac cctcgcaggc gagcgggtca
     7861 atcgcgcacg atgcgctgat ggcgtcgaag cggcacaggg gggcgcaggt tccgcaccgg
     7921 tcgcagcgcg cgggatcgat gaacgccttc ttcccgccca caaaccgttc gcgctcgacc
     7981 gtcgtcccgc cgagaacgag cttgaggtcg gctgcgtcga cgtcgcagtc ggcgatgacc
     8041 gcgttttccg cgagcgcggc gaacgacgcc gtcacgctcg ttttaccggt tccgcccttg
     8101 ccgctgatga tgacgagttc cttaatcatg cgcgtcccgt ttccgcaaaa tataaaatac
     8161 cgaccgcaga ttctccggcc gcttcatctg ctcttcgatt gcggccaatt cgtcctccgc
     8221 ggcacgcacg agcgaatcgt cgccacgagc gcggccggaa tccccgagcg cggccagtgc
     8281 ggtcttcatg cacttgtagt agtcgggcca ggcgttctcg gggagaacga acgaatcaac
     8341 aatcccatag ccgtgcttct cccacagccg ctttttctca tcgccgcgcg atatctcgtc
     8401 atggataacg agatatcctc ccggcttgag cagttcgcca gcgcgggcga gtcccgcctc
     8461 gaaaccgatg atattgaaaa ggccctccgc ccacacgaca tcaaatgggt ttttctggat
     8521 ttcgggcgag gcgaaatcca cgttcatcac cgtcatgcgg ttggcaaagc cgtcacgcgc
     8581 gaccttccgg cccatcgcgg cgcacgatgc ggcatcggga tcgatcgcac agatacgcgc
     8641 ggcgctcatc cgcgcaagcg ccagggcggg ctccccgctc ccgcacccgg catcgaggat
     8701 gcgcatgccg tcgataggcg gcagcagcga aaacgcacgc acggtgtatt cgttgagcgc
     8761 cgcgcgacag gcgtcgccga tcgaacgcag ctcatcccgc atgggtcccc ctatcgtgat
     8821 tcgcggcggg cgcaatcgca gccgccacga gcgcgatctg ttctccgtaa cactcgatga
     8881 tccgcgacgc gcaatcgccc cgggaatact cctcggcgat cctgcggtcg aacggcacgc
     8941 gcgcgactat ccggacgtcg ttcactccgc accagcgctc gagatcggcg ttccccatcc
     9001 cgtcgcggtt gatcaccacc ccgaagggga ggcccatggc gcgcaccatg tccacggcga
     9061 gcatcaggtc gttgaggcca aacggcgtcg gctcggtcac caacacgaga taatcggccc
     9121 cccgcgcagc ctcgatgacc ggacacgagg tgccgggcgg gcagtcgagg attcgaacat
     9181 cgtatgaccc atatgcccgc ttcacctggt tgataagcgg cggcgagagg tgcccgccga
     9241 tggcgagcct gccgcccgca tagtcgatgc cgttgcgcga tccgtattcg atatcgccga
     9301 tctctttggg aacctccgtg atcgccgcgg tcgggcagac gagggcgcac ccgccgcagg
     9361 agtggcacat gtcgggcagc acgagcggcc gctcgttgat gaggacgatc gcgctgaagg
     9421 cgcaaatgcg ctcgcacgcg ccgcagccgg tgcacgccgc cggatcaacc gaggggacgg
     9481 ggacgcccgc gcgctccgtg cgttcgatcg cggccccgag gaagaggttc acgttcggct
     9541 cctcgacatc gcagtcgagc acggcgacgc gcgaaccgtt atgcgctata tatctcgcca
     9601 gggacagggc gatggtggtt ttccccgtcc ctcccttgcc gctggcaacc gcgatattca
     9661 tgcccgcccg ccgccctcag aactcaaaca ccgcacccac cgaggcgggc gtgaacgcct
     9721 cgggaaacgc ggatgtgatc atcagggcgg cccgtgagcc cgtgcagtgc gaaacgccga
     9781 cccgccggac ccgataccgc tgcagcgcat cgatggtccg gtacacgtac tccaccggcg
     9841 cgctccccag gtgcgtgccg ccgatgacgg cgtggaagct cccaaagccc gtctcccgcg
     9901 aaatcgcatc gagaatctcg acgatccccg cgtgggcgca accgagcaac accaccggcc
     9961 cggaatcggt ctcgacgcac agcgacagat cgtcggtgaa gggatcgtcg acatatgcgt
    10021 cgccttcttt tctcttcagc atcgcatcga atcccctcca cccctcgggg cgcgggattg
    10081 cggacaatgc gtgcatgccg tcctcgatcg cctcatgtcc ggtcgtatga tggaaatcgc
    10141 agccggcgcg ttcatactcg ccgcgctcgc ggggaaaccc gatcggcagg gccgactgtg
    10201 cgttcgcggc gccatagacg gcgaatttcg ggatgaacgc gtccgggttg acgaagaccg
    10261 gcagcgcggc atcgcgcccc tgtgcgaacg cgagcagccc tccggtatga tcataatggc
    10321 catggctgag aatgacccgg tcgactgctg ccggattttt cccaagaacg gaaacgttat
    10381 gcatcagccc aagaccctga ccggtatcaa agaggctcac gccatctcgt tcgatcagca
    10441 tcgaaagccc atgctccccg atcagcggga aagggacatt aacgctgttt tcgcacacaa
    10501 tcgttatccg tgttttcata tcaccaatgc ccctcgacgg tcgcctgcga cgcctgcgta
    10561 agtttaccgt cctggaagtc ctgtatcgcc tggtcaacgg tcccggacgc acccgtaaac
    10621 atatccacgt gcgccgcgat gagcgccgca tgggccttcg gccccacatg gccggtgatg
    10681 agcgcggaga cgccggcatt gatcacggtc tttgccgcct ggatacccgc gccctgcggc
    10741 gagtccatgt tctgcgcatt gtcgatatat tcggacgcgc ccgattccat gtcatggacg
    10801 atgaaaccct tggcgcggcc aaaccgctca tcgacgagcg actgcgtgtc cgttccggtg
    10861 ctggtaattg ctattttcat tgttctcctc cgctcgtgtc cgctcgtgtc gttcatgcag
    10921 tgcatgcgcg attttccggt tgacatttta tgagcatatg cccatatgga ttcaccgggg
    10981 taaaatgtca aggccaaaat gctgcagaaa ggtcggtatt gtaccgacga ccacgatcta
    11041 ccgacccgag ggcgacatgc ccttcgccgg cggggagatc gttttgtcgc tggacgagtt
    11101 cgaggcgata cggctggccg acctctcggg cctgtaccag gaggatgccg cgaagaagat
    11161 ggacatatcg cggcagacct tcggccggat cgtggaatcg gcgcaccgga aaatcgccga
    11221 cgccctcgtg aacggcaagt ccctgcgcat cgagggcggc gcggtgtcgg tcgaaacgcc
    11281 gccgcgctgc aggtgcgaca agtgcaaacg atcgcttgac tgcccgcatc gtttccgcga
    11341 ggacggctgt ccgaggatcg ggcacggaaa ggaatcgatc aaccccggtc agtaatcaaa
    11401 cggaggcaca tatgaaaata gcggtaccgt cgaatcaaaa ccaggtggac gcccacttcg
    11461 gacactgcga atactttacc gtgttcacag cgaacgataa aaacgagatc gtgagcgagg
    11521 agaaggtgat gtcgccggcc ggctgcggct gcaagacgaa catcgtcggc acgctctcgc
    11581 agatgggcgt gaaggtcatg ctggcgggca acatgggcga cggcgcggtg aacgtgctta
    11641 acagccacgg gatcaaggtg gtgcggggat gcgccggcac cgtgaagtcc gtggccgagc
    11701 ggttcctcgc cggcgcggtt tccgattcgg gcatcggctg tcacgagcac gagcacggct
    11761 gccacgaatc gtgaaccgga tgaaaaccgt cacgcagctc cccatcatag atttcgcgaa
    11821 ctgcgtgaag tgcatgaact gcgtgacggc ctgtccgcgc tcggcgatcg tcgagccgat
    11881 caacacctcc tgcgccaagt gcgtcaagta ctgcatatcg atggaggtcc cgtgcagccc
    11941 ggagaacatc atcttcgact acgcgctctg cgattcgtgc ggggaatgcc tgtcggcgtg
    12001 cccggaaggc gcgatctact ggttcacgcc gtctgcatag ggccccaacg gtatgcgcaa
    12061 tgatcgggcg ccgacaagag aaaactcatc ggcccccgat catctgatcg tcactcgtcc
    12121 gctcccttcg ggtgaagaga tcggaaccct atccccgttt cgcacgccgc gggatgattg
    12181 tcacgagaag cgccaggagc gcattgcaga agacgatggt gtagccggtc gggaaatcga
    12241 accggtgcgc tagcgcgatg gccacgagat tgaccagggt gccgacgatc cacgccacga
    12301 cgagcggcct gccgccgccc acgcggatgg cgatgagcgc cggcgccacc aggagcgcga
    12361 agacgataag cacgcccgcg agcttcacgg agctcgtcac ggtgaccgcg aaggacacaa
    12421 agaacagcgc atccttccag aagccgtcga tccgtcggta cagcgcgaac aggaccacgc
    12481 ccagcaccgc gtagatgatc gcggttttga ccacctcctc ctgtggcgta aacaggatgt
    12541 ccgaggccat gagacgctga aactcctcca tgccgtgcgc ggatttcgag aggataatgt
    12601 acaccgccga aatcccgaag gcgtacatga gcccgatgaa cgcctcgagg ttgtcggtgt
    12661 gccgcgaggc ccacgcaatg agaagccccc cggccagcgc aaagcccagc gatatcggat
    12721 acacgaactg cccgtcgatg aaaaacagcg acgcggccgc acccagcgcc gccatctggc
    12781 cgatggcgag gtccgtgaag atgatgcccc gccggatgat ctcggtgccg aaaaaggcgt
    12841 ggatccccac cagcaccacc gaaagtgcga acgccgttat caggatatcg ctcattgcgt
    12901 caacctccgc accatttcat cgaacaacga gaagatgccc tctgcctccc ggaccgcgcc
    12961 gacatcgtgc ggcaccttga ccatcttcac gccgaacttc ttcgaaaggt gccgactgga
    13021 atcgtccgcg ttgtagacat cctgtagaat gaacctgatt gtgttcgact cgaagatctt
    13081 ctcgagctcc gcaatgtgct ttgacgtggg cggtatcccg ggcagcggct ccacggttcc
    13141 catccgcacc agcccgtacc gaatgagcag ataatccatg ttcttgtggt actggatcac
    13201 cttcgcgccc ttcagcctcc gcagcttcgc gtcccaggac gcgcgcttcg ccttccatcg
    13261 cgcggcgaac tcatcatagt ttttcttgta catggcggcg tttcgcgaat cgacctccga
    13321 aagcttcgtg cagatcgccc gcgcgagtcg cgggatcaca tgcggatcga tgtaataatg
    13381 cggattcccg tccgggtgga catcgccctg cgcccgtgat acgctcttgg gaacgtcgat
    13441 gagcgtgaca tgcccggaga gatcgagaaa cccccgcgtt ccgggattca ccgacgcgtt
    13501 gttcgcctgc ttcaacagcg gcggcagcca gccgatctcg agctgggcgc cgttgatgat
    13561 caggaggtgc gcctttctca gtttcgcgat gaaggacggc ttggggacga tggtatgcgg
    13621 atcgtagtct ccgcgcgcga gagcgtccac cctcaccgtg tccttggcga tctgctccac
    13681 gatgctcgcg atgtagggat aggtggtcac caccttgagg cgctccgcgc gcagcggcgc
    13741 cgcgacggat gcgatcatcg cgacggccaa cgggattagt actgttcgtt tcatagtgcg
    13801 tctcctcacg ttagaatgca tgggcgccgt gggcgccgat ggtaatgttc acttgcagga
    13861 ccacctcatg gttgggcctg ctcgcgaacg agccgtcccg ggggacatac atgctgtgat
    13921 cgtagttata ctggagcctg aagcgcgaga attcggtcgc gttgaaatcg accatcgcgc
    13981 tgtagcgcgg cagcgattcg ggcaggctct ccttgatgcc gtcgagtttg atgtcgttca
    14041 ccgcgagaag gtcatagcgg atgcccgcgc gccagcgcat ggcgaacttc cccacgagct
    14101 gcgcgtacag ccccgactgg cgcttcacga gcgcgtactc ctcgacgata tccgtctcgt
    14161 tcttcgcgta atacgcgccg tccatgaacc ggtagaggta ctcggtctgc agcgcgcaat
    14221 agcgcttgga atcgaattgg tacttcaggg taaggtcggc gcccagtaca tggctgtgcg
    14281 cggcgatcgc ggtgcccgca acgccagcct ggtccacgcc atgattgatg cgcgaccgcc
    14341 cataggcgcc cgacgccccc atgtacaccg tgaggtcgcc gatatcgaac gaggtcttga
    14401 tgtatccgac gccgaggttg ggatcgtcgc tgtcgcgcac gcgcatgatt tcattgggat
    14461 ccgcgaatcc ggtcgcgccg aagctcgccg cgttcgcccc ctggagcgcc tcggcgccga
    14521 tcatgaggta gaagtccagc ggggcgaccc aggtggcccg aacgcccacc tcgttgagat
    14581 cgccgccgaa gaaggcctca tacaccaggg gcgcatcgta gaagtcccag ttgtgcgcgt
    14641 gctgctcgtt gatgcgcccg aaactgctca agaatttccc cgtcttgagc tggaagccaa
    14701 acgggagcga cgtcgtacag aaatacgcct cctcgatgag gaccttcgcc gtcgtgagct
    14761 cgatcgccgc gaagaggtcg aagtacgggt ccacgaccga atagagcgtg agctcggcgt
    14821 aattaaagtt gaatccgcgc attaccgcgt tgggcgcgac gtgctcgtgc tcggcctcgc
    14881 ccgacatcga ctggatgaaa ccgggacgca gttgcgtttt gtatatttcg ttatcacggt
    14941 cccggtgatt gtagctcgcg tcgagcacga atgagatgtc cgggacgaac tgggactgcg
    15001 aaaagggcga agaggtcgtc ccctccttca tggattgggc atcgcgtttc ggagctgttc
    15061 gcgccgcgcc ccgttcctcg tcatcatagt tttcaggctc gtcgggtgac ccgttccccg
    15121 gatccttcga ttcattgcgc ggctcaagcg ttgcatcgtc gtcgtcatag ttcggcaggt
    15181 cggccggaga gtcatcccgc agctccttct gggccggctc cttcggagca tccggcctgt
    15241 cctggctgcg cagagacggg atggcaacgg aaaaaataaa tacaagcaat atgacagtaa
    15301 atcgtttcat caaacctcct tcagggtata atcacatcag cgcgcatcaa gggagatgcg
    15361 cgccgtcacg cgccgggacg aacgaagtcc cgcgcatgcg acaggcggcg acgcatacgc
    15421 gttaccgtgc ctgacaggtg ttgttatgcc gtgaagggtg gcgcgttagg gcgcgaggcg
    15481 aggagtgcgt gattatgaag cgaaaccgat tcatatacga tgaaaacatc tacgtggaca
    15541 aaatcgggcg tactcagcac gcatgcggac ccctgcgcct ttgcggtgaa acccgaatcc
    15601 tgaaaccggc acagggggca gtcgtcaatg atgacgccgt tctcatgatg atggaacgca
    15661 accatcgcga tgtcaatcgc gaggaccgca gccaacaata gtgcaatgta cgctagcttc
    15721 ttcatagata cgttcgatgt atgcccgtaa ctccgatcat gaccatctgt caagcggaaa
    15781 ttaccactgg tcaccttgcg agcaattccc agcacgcgtc gaagcccact atagcaatac
    15841 tgtcgggagt atcaatattt ttcataatgc ccggaaatcg catcaaccag accgtcgatg
    15901 gtgtacaccg gcgcgacgac cgcgggatca tgcccgagct ccttcagcgc atcggcggtg
    15961 atgggaccga tgcaggcaaa ctgtgcgcgc gactccttta cgatggcgaa gaagttccgc
    16021 accgtggacg agctcgcgaa ggtgaccatg tcagcgtcgc gaacatcggc gagcgcgtcc
    16081 ggatcgatcg tgtccggaac gaccgtgtcg tagagatgaa tgcgctcgac ggtcgcgccg
    16141 agcgaccgga gaccgtccgc aagcgcggcc ctcgcctccg tggcgcatgg cagcagaacg
    16201 cgcgatcccg cgacgccgac cgttttcatc gcatcgatca ccgcctccgc gacatactcc
    16261 gcgggaacga ggtcgggatt gatcgagcgc accctgagct cgttcgccgt cgcgggcccg
    16321 atcgcggcga tgcgagcgcc ggcgagagcg cgcgcgtcca tcccggcggc cgcgagctcg
    16381 tcgaagaata tcgacacggc gttctgcgag gtgaagacga tccagcggta ggatccgata
    16441 tcggccaacg ccgctcgaag atccgagaga tcgcccctcc tggcgattcg gatggttgga
    16501 aactccacca catccgcgcc cagcgcggcg agcttctcgg agaggaccga ggcctgttcg
    16561 cggatgtggt ggagtttcca accat
//
LOCUS       DAORHF010000054        16389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2630_length_16389_cov_5.539794, whole genome shotgun sequence.
ACCESSION   DAORHF010000054 DAORHF010000000
VERSION     DAORHF010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16389
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2630_length_16389_cov_5.539794"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="PKJ16_06665"
     CDS             complement(<1..1341)
                     /locus_tag="PKJ16_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV46706.1"
                     /translation="MDTIRDILRSVPLFGGCSAVELDSLRRMARAASFERGRRVDVGA
                     ANSLFVVAGGTFEIESAGPGDPVFLAPGSFFGDLPLARAPRKGAVRARTDGTLIILQS
                     DDLTRLLLSSFKAFRAYADALAYLDIAHAGAEGADAVARCRVITVFGPGEGSGKSFFA
                     ACLGAALPSGERAVILDASYRGVSVFSVLGCKMMPAISQRQSAEVIDEKFIFDRVVRA
                     TDSLSAINIVAGSKVRIDPSILPPILYLLAREYRYVIIDLSDYDEDLRAAALAASDIV
                     FCLLQKEKDRRRVYDMLAAELAEGQRLFYVLNAHNAGQNARIDGGLLFEAVDMPDDGV
                     SLTAHAQRLREGPMKEFVAAVTARRRGLVLEPGLLESVAHAGALAALREAGRTFDVIY
                     AASMSFAVAGLYLLSSDNDSFRDAVLRLFSEERLNALLDISFPEEFVFRNTKLVR"
     gene            complement(1352..2710)
                     /locus_tag="PKJ16_06670"
     CDS             complement(1352..2710)
                     /locus_tag="PKJ16_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46707.1"
                     /translation="MLFTYIIIFFFVIVFAIFALYLLNTYVFPRRIDEIAQMIESGQT
                     KLAIKKLTDMLEKDDRDAYAHFLLAEAYRKENNIQYAILEYRQVLKLGKFDAVVSEID
                     VRTKLAAIFKDKGAVEEAKKEFLILTKIDPDNYRNYHELGVIYFNANVFDRAGGYFKK
                     SISCNAQDSDSQYYLGQIFYRSGNYPEAKQMFIETLKIDQTNYRAHYFLGLVLRQLGD
                     HEWAVKEFEVAQKSDDIKVKCFLAKGTIFLEKEQYPKAVVELERGLKFAKRRSDTELN
                     LRYFLAEAQEKMRDLHSAIFNWEKIVEVNRDFRDVQEKLKSYSEFRQDDRIKDFMIAG
                     LSKFEHLCRKICEGMGLTIMDVDIISDTVIEILATETEGKWRNTRRTNRIIRIMRNTD
                     SVGDKLLRQLHESMKLRNATRVIIISTGEYSQSAVDFSNTRPIELLGKNELVGLLRNV
                     KD"
     gene            complement(2736..3059)
                     /locus_tag="PKJ16_06675"
     CDS             complement(2736..3059)
                     /locus_tag="PKJ16_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46708.1"
                     /translation="MADQLEKLTKENEALKRENEHLKNMLRSYENVLKLNEQELENAD
                     EIKKMYENICEFSRTELMQAVETARAQESASELSREELLKAFHHIKDLEEENKRLKEQ
                     RASAR"
     gene            3155..3227
                     /locus_tag="PKJ16_06680"
     tRNA            3155..3227
                     /locus_tag="PKJ16_06680"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3188..3190,aa:Ala,seq:ggc)
     gene            complement(3305..4528)
                     /locus_tag="PKJ16_06685"
     CDS             complement(3305..4528)
                     /locus_tag="PKJ16_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HNV46709.1"
                     /translation="MARLKAFRGLRPAPGLAEQIAELPYDVVSSDEAREIARNRPLSF
                     YHVTKPEIDLPETIDLYDDRVYDTAQKNLRGFIEQGRLRPDGAPALYLYSLSVDGREQ
                     TGLVACVHIDDYANKVVKRHELTREDKERDRTRHMAALGAQAGLVYLFYREDGSKADL
                     FARARAAAPECDFTAPGGVRHVLRALPDAALVQSFVDAFANDVLYIADGHHRAASAVR
                     LGRERRSAHDDGSAEHNWFMAAIFPHAELRIMAYNRVVRDLHGLSPEAFRARLGERFT
                     VTPAADGVPSAKGRFCVYLDGAWLELTPRFAVNDPDPAKRLDVRTLQDEVLGPILGID
                     DPRTSTRIDFVGGIRGTGELERLVASGEFRTAISMYPTSIEELMAVSDADGIMPPKST
                     WFEPKLLSGLVLHQI"
     gene            complement(4530..4664)
                     /locus_tag="PKJ16_06690"
     CDS             complement(4530..4664)
                     /locus_tag="PKJ16_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46710.1"
                     /translation="MDKKVVDFISYKIEKSLRRSGFSVKKDNKKNVKMLLKLNDKHRG
                     "
     gene            complement(4914..6329)
                     /locus_tag="PKJ16_06695"
     CDS             complement(4914..6329)
                     /locus_tag="PKJ16_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46711.1"
                     /translation="MAGRCRMLLAVAALLVAVDARAVVISRIVIVGSSVDIGLAELVA
                     GRTGDLSDADIRAIGVEVTRRYHEAGYVTCHVARLVARRDGALEVHVVESRVREAAVA
                     GVDGPAARELAALMLPEPGEIYNRIVVAERARFASAALGLSSVRVSAENHGDGADVLL
                     RITALVAPRGAVGFGMGYGPRYGYAPVIEYARPAGGGAVRSRAEIGFRDGYLRQGIAA
                     VEFDGRDRETDGGGLLLGARYRHARDVWESMDREFLSNAGEAFAGYGWRFGSASLDCT
                     FRQAMSGLERYRGNWYVDNDSRAQAVYRIADRRLLLDRRRASELAFSASGGASDLCPK
                     GYAMAAASGRTALVPVPWLFVVPHFDAWYATSAERYHWRYVFDETLPGRSDDFTASRW
                     KHTAGIDLLFEAYPDLAMAGPFAVAGWYRNEDDEWRTAVVCGVRCTVQFRGVSIAAAW
                     SWDVSGPLSRGSVTFSAAGSL"
     gene            complement(6329..7069)
                     /locus_tag="PKJ16_06700"
     CDS             complement(6329..7069)
                     /locus_tag="PKJ16_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46712.1"
                     /translation="MGMGTVFVLMGVLAAGATDGRDLLRELVGRCAGVSSIDAVIEQH
                     IRYPGAPPEVFRGRYRADAQGRFRIDYTAPSVQRVLHTGAALYWYYPADNLLYRIGGG
                     GGSAPAPGARPADELMRRLDERGEVEYLGRRLYGIFTLAHRFVVRGDDPRVRIEVLVD
                     SRRGVVLERRLLDERGREIVTEVFSDYDRTQGACYPRRIDVRARGERGVVRSTTVYRD
                     ARINIPLSPGLFAPDFPPNARRRLYRDR"
     gene            7334..7582
                     /locus_tag="PKJ16_06705"
     CDS             7334..7582
                     /locus_tag="PKJ16_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46713.1"
                     /translation="MHIPDASSPFWISAVTSERTYELEFLAMKILLARLRLAVKQDAS
                     PESIQKCAHELRELFLKSQHLPKAQNDLKRMIQEGGMS"
     gene            7579..8568
                     /locus_tag="PKJ16_06710"
     CDS             7579..8568
                     /locus_tag="PKJ16_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009580106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46714.1"
                     /translation="MNPVLYGLDEVAQRITTGARLVLAGDEAVLSKLPKGRWIGGTIP
                     YFMGADGGVMSKDLIHVTELPASVETASCTTYRADTIARVYADAPANGFSIIIIPAAS
                     PTHLSFAINAPTYEGFATSPLIGWISGVHVDEIGKRRAKVFFGETGEALEDGAVVMQA
                     TLPAGTYADLNIINIFSQGDGDTITFPEDGFIVGDAFINGENTNFADYIAARTLDTRL
                     PLVANYCGAMINISIQRIDTANRRVHLYAPVFRDVQYRHAAPVGDYVREFTGHLPHDG
                     IDSIFFSCNCILNYLYSELEGRKTGGITGPITFGEIAYQLLNQTMAYLTIRSL"
     gene            complement(8565..9857)
                     /locus_tag="PKJ16_06715"
     CDS             complement(8565..9857)
                     /locus_tag="PKJ16_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46715.1"
                     /translation="MAAEEKLTPTRVGVQPPRDPILVVEDNEETQALLKGILKKMQIP
                     CVVAGNGREGLERVGQGAFSLYIVDLMMPVMDGKQFIKELKKRDPNAVVLVQTGLDLS
                     ETIISVMRLGVYDYLIKPIDPEVFQAIVWKAVEYKHLKDLEEGQRASASLKIRSQIEW
                     LNYKESRRVADRDYSSTKSIYSLKTSMSQGAGIGTLVTLIELLKSAAEPQGDKYLVDK
                     NIVDIIIENNDFCRLMIEGLQMVSDIIENEVPLSRAASTDLIEALPGMIEGVLPYMEA
                     RRIGVTYPEPRAACPLDLNMEKVALAVEELMINAYKYSVPGSVINIIARQSEGYFWLS
                     VKNEVSEKPYCGIPENYEKMVLEPFFRILPPDESIAKYEKFGSGLGLTVVDNIVRKHK
                     GLFMIRDIRDLTGRAPKLCVMAEMLFPVADAASAAERG"
     gene            complement(9946..11946)
                     /locus_tag="PKJ16_06720"
     CDS             complement(9946..11946)
                     /locus_tag="PKJ16_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46716.1"
                     /translation="MKKSVLIVEDEQITASSLQGVLSRLGYAVAGIAPTGEAAVEMAA
                     RLRPDIVLMDIMLGDGIDGIEAASRIAAHSDVPVVYVTSYSDEATLLRAKITAPFGYI
                     IKPFTERDIATCLEITLYRKEIERRLRDSEERFRVLAGAARDAIVMGDEDARVVFWNR
                     AAETMFGITSDEAVGRPIPELMVPARMLDEFSRNFSMYRDPARGMTADDVFEAVVMRR
                     DGGEFPVEIAASALELKGRWHVMGLLRDITRRKKQDAERQRFVEIITGRLKALDCLYK
                     VARLVERADRPLDEIFRTTAEHLRTALRASARAGARIEYDGAEYRTESFAETASMLAA
                     DITVRGAVRGRVQAGYVDGPDGRAAVPFDDEEVHLVDAVARELGVTAERKIAEEELRA
                     YQENLEDLVRRRTAELTEANRLLSAEIELRKATEDELREATLAAMAASRAKTEFLSNM
                     SHELRTPLNSIIGFAKLMKMGYDPDDYGPNLDKIIFSGTHLLKVFNEILQISRIESGR
                     FEIERKPVDLARIIAECGGMMEVQAAKRNIAISIDAAAAAGAQILGDEAKLQEVFINL
                     LSNAVKFTDEGGRVAVRARTASGALEVDVSDTGVGISPEHAEYVFEKFARVETGLTRK
                     TEGTGLGLSIARKIVEAHGGAITLKSAPGEGSTFTVRLPIAP"
     gene            complement(12125..13045)
                     /locus_tag="PKJ16_06725"
     CDS             complement(12125..13045)
                     /locus_tag="PKJ16_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HNV46717.1"
                     /translation="MIAVGSGGAAHGRDDRYPVERISAEFRRRFPGMRRAEIPPDCVR
                     RFRAVIRAYYRRYARDFPWRRTDDPYHILVSEIMLQQTQTARVAERFPAFIASFPTVR
                     SLADAAQREVLAAWQGLGYNRRAAYLHRMAREIVARYGGCVPDAEEELRALPGIGAAT
                     AASIAAFAFDRPTVFIETNIRAVFIHFFFHGRSGVRDGQIAPLVARTLPRRGAHVWYS
                     ALMDYGAMLKRRYPNPARASAHHARQARFEGSRRQLRGAILRIVLERPGASVAHVARM
                     TGRTRDEIRDVAGELAAEGLINMKRGMLYG"
     gene            complement(13042..13398)
                     /locus_tag="PKJ16_06730"
     CDS             complement(13042..13398)
                     /locus_tag="PKJ16_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46718.1"
                     /translation="MYATLTDEQARDAIARGEFAPALVGAAPRTVMVLTQDWCPQWAA
                     MKDWLADAADEAGVAVIVFEYNRSGVFEEFMRFKEDVWGNRQIPYLRYYREGALVRES
                     NYVSRDAFRANLAGMR"
     gene            complement(13647..14366)
                     /locus_tag="PKJ16_06735"
     CDS             complement(13647..14366)
                     /locus_tag="PKJ16_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HNV46719.1"
                     /translation="MIIQSTDTDLFRSCRTLFSEQTRITVDFLRLLSLSDIKSAYRNR
                     VMENHPDRAKLLGRDPDELERHFRAINEAYLHLAEFVQKNNSRSSTTEITHRFSFRAQ
                     ASGPVKPIVPATELLLGQFLFYQRRISINTLADAVFWQRKQRPSYGKIAIDWKIMTVQ
                     DITRILAARDHNEKIGEYALRNGYVTDLEHKAILGRQHMLQKPIGCFFVERGIVDPEE
                     LQDLVRQLRRHNAAVRRMRSH"
     gene            14849..15700
                     /locus_tag="PKJ16_06740"
     CDS             14849..15700
                     /locus_tag="PKJ16_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014933443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNV46720.1"
                     /translation="METIQTPQGVLAISDEEFEFFRSIVYEESGIDLSDKKKALLQAR
                     LTKRLRELRIRTYHEYIGHLKSHFHDEIEHFINCITTNKTEFFREGGHFEFLRTTVLP
                     AYEREKRRRIRIWSAGCSTGEEPYTIAIVLLDYFDGRALPDVKILATDIDSRVLETAK
                     AGTYKAESVHMIGLDMLRRYFYRGTGENQGLFKVKHDVRRLISFRRLNLLDESYPMKN
                     TFDIIFCRNVIIYFDKDTQNALFAKFHRYLADDGYLFMGHSEALIGRSSLFQLKTNAV
                     YEKSGNR"
     gene            15739..>16389
                     /locus_tag="PKJ16_06745"
     CDS             15739..>16389
                     /locus_tag="PKJ16_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46721.1"
                     /translation="MGKVKVLIVDDSAVMRKTLEKSLVRSKDIEVVGTALDPYIAVDK
                     IKKLQPDVITLDIEMPRMDGLTFLSKLMIATPMPVIMVSALTDAGATVTIKALELGAV
                     DFILKPQLEDAAGWDEFSRELVAKVLAAGGAHVKRKRTDAPAPGAVTISVDQKFSADV
                     ILPKKRPDLAKAHTGTVIAIGASTGGTEVINEILMGLGEDAPGIVIVQHMPEKFTKA"
BASE COUNT         3006 a         5359 c         5104 g         2920 t
ORIGIN      
        1 tctgaccagc ttcgtgttcc ggaaaacgaa ttcctccgga aaggagatgt cgagcagcgc
       61 attgaggcgc tcctcggaaa agaggcgcag cacggcgtcc cggaacgagt cattgtcgct
      121 cgacaggaga tagagcccgg ccaccgcgaa cgacatcgac gcggcgtaga tgacatcgaa
      181 agtacggccc gcctcccgca gcgccgcgag cgctcccgca tgcgccaccg actcgagcag
      241 accgggctca agcaccagtc cccgccgccg cgccgtgacc gccgcgacga attccttcat
      301 gggcccttcg cgcagtcgct gggcgtgtgc ggtcaaggat acgccgtcgt ccggcatgtc
      361 gaccgcttcg aagagcagac cgccgtcgat gcgtgcgttc tggccggcat tgtgcgcgtt
      421 cagcacatag aacagccgct ggccctctgc gagctcggcg gcgagcatat catacacgcg
      481 ccgtcggtcc ttttcttttt gtaacaggca aaagacgata tccgatgccg cgagcgccgc
      541 tgcgcggagg tcctcgtcgt aatccgagag atcgatgatc acgtaccggt attcccgggc
      601 gagcaggtac aaaatgggcg gcaggatgga cggatcgatc cgcaccttcg agccggccac
      661 gatgttgatc gccgagaggc tgtcggtcgc acggacgacg cgatcgaaaa tgaacttctc
      721 gtcgatcacc tcggccgact gccgctgcga tatcgccggc atcatcttgc acccgaggac
      781 ggaaaacacc gagacgcccc gatacgaggc gtccagaatg acggcccgct ccccggacgg
      841 cagcgccgca ccgaggcagg ccgcgaaaaa ggacttcccg ctcccctccc cgggcccgaa
      901 caccgtgatc acgcggcagc gggccaccgc gtccgcgccc tcggcgccgg catgcgcgat
      961 gtcaagatac gcgagggcgt ccgcgtaggc gcgaaacgcc ttgaacgagg acaagagcaa
     1021 ccgcgtgaga tcgtccgact gcaggatgat gagcgtgccg tcggtgcggg cgcggacggc
     1081 gcccttgcgc ggggcgcgcg ccaacgggag gtcgccgaag aacgagcccg gcgcgagaaa
     1141 caccggatcg ccgggccccg ccgactcgat ctcgaaggtg cccccggcga cgacaaacag
     1201 cgagtttgcc gcgccgacat ccacgcgccg cccccgctcg aatgaggccg cgcgcgccat
     1261 gcgccgcagg gagtcgagct cgacggccga gcagcccccg aacagcggga ccgatctcag
     1321 gatgtcgcgg atcgtatcca tcgcggcgcg atcagtcttt gacgttgcgc aggaggccga
     1381 ccagctcgtt tttgcccagg agttcgatgg ggcgcgtatt ggagaaatcg accgccgact
     1441 gcgagtactc gccggtcgag atgatgatga cgcgcgtggc gttacgcagc ttcatcgact
     1501 cgtggagctg ccgcagcagc ttatccccca cgctgtcggt gttgcgcatg atgcggatga
     1561 tgcggttggt gcggcgggtg ttccgccatt ttccttcggt ctcggtggcc agtatctcga
     1621 tcaccgtgtc gctgatgata tcgacgtcca tgatggtgag ccccatcccc tcgcagatct
     1681 tgcggcagag gtgctcgaac ttggacaacc cggcgatcat gaagtccttg atgcggtcgt
     1741 cctggcggaa ctcggagtac gacttgagct tctcctgcac gtcccggaaa tcgcggttca
     1801 cctcgacgat cttttcccag ttgaagatcg ccgagtgcag gtcgcgcatc ttctcctgcg
     1861 cctcggcgag gaaataccgg aggttgagct cggtgtcgct acggcgcttg gcgaacttga
     1921 gcccgcgctc cagctccacc acggccttgg gatactgctc cttctcgaga aagatggtcc
     1981 ccttggccaa aaagcacttc accttgatgt cgtcgctctt ctgcgcgacc tcgaactcct
     2041 tcacggccca ctcgtggtcg cccagctgcc gcagcaccag ccccaggaag tagtgcgccc
     2101 ggtaattcgt ctggtcgatc ttgagcgtct cgatgaacat ctgcttggcc tccggatagt
     2161 tcccgctccg gtagaatatc tggccgagat agtactggga gtcgctgtcc tgcgcgttgc
     2221 acgaaatgct cttcttgaaa tagccgccgg cgcggtcgaa cacgttggcg ttgaagtaga
     2281 tgacgccgag ctcgtgatag ttccggtagt tgtcgggatc gatcttggtg aggatgagga
     2341 actccttctt ggcctcctcg acggcgccct tgtccttgaa tatggcggcc agcttggtgc
     2401 gcacgtcgat ctcgctgacc acggcgtcga acttgcccag cttgagcacc tgacggtact
     2461 cgaggatggc gtactggatg ttgttctcct tccggtatgc ctcggcgagc aggaaatggg
     2521 cgtaggcgtc tcggtcgtcc ttctcgagca tgtcggtgag cttttttatc gcgagcttcg
     2581 tctggccgct ctcgatcatc tgggcgatct cgtcgatgcg gcggggaaac acgtaggtgt
     2641 tgagcagata caacgcgaat atcgcgaaca cgataacgaa gaagaagatg atgatataag
     2701 tgaataacat cggttgacgg gtgccgggcg ggccgttagc gggcgctcgc ccgctgctcc
     2761 ttgagtcgct tgttttcctc ctccaggtcc ttgatgtggt ggaacgcctt gagcagctct
     2821 tcgcgcgaca gctcggacgc gctctcctgc gcgcgcgcgg tctcgacggc ctgcatgagc
     2881 tcggtccgcg agaattcgca gatgttctcg tacatcttct taatctcgtc ggcgttttcg
     2941 agctcctgct cgttgagctt caggacattc tcgtagctgc ggagcatgtt cttgagatgt
     3001 tcgttttcgc gcttcagggc ctcgttctct tttgtgagct tctcaagttg atctgccata
     3061 ttgattacac cccttcggga aaatcacccg tgcatcgaaa aaatagtatt gacaaaaccc
     3121 aaccgttcaa acggtaatac ccaccgaacc gaacggggat gtagctcagt tgggagagcg
     3181 cttgaatggc attcaagagg tcaggggttc gattcccctc atctccacag gaactatacc
     3241 gcagtaagca caacccagcc gaaataaaaa gtcaagtcaa ttctttctcg aacgcaaccg
     3301 accgtcagat ttgatgcagc acgaggccgc ttaacagctt gggctcgaac caggtcgatt
     3361 tgggcggcat gatgccgtcc gcgtcagaca ccgccatcag ctcctcgatg gaggtcgggt
     3421 acatcgaaat ggccgtccga aactcgccgc tcgccaccag ccgctcgagc tcgcccgtgc
     3481 cgcggatgcc gcccacgaaa tcgatgcgcg tactggtgcg cggatcgtcg atcccgagaa
     3541 tgggcccgag cacctcgtcc tgcagcgtgc gcacatccaa acgcttcgcc gggtccggat
     3601 cgttcaccgc gaaacgcggc gtaagctcga gccaggcccc gtcgagatac acgcagaagc
     3661 ggcccttcgc ggagggaacg ccgtccgcgg cgggggtgac cgtaaagcgc tccccgagcc
     3721 gggcgcgaaa ggcctcgggg gagaggccgt gtaagtcgcg caccacccga ttgtaagcca
     3781 tgatgcggag ttcggcgtgc gggaagatgg ccgccatgaa ccagttgtgc tccgcgctgc
     3841 cgtcgtcatg cgcgcttcgg cgctctcgcc ccaggcgcac ggccgaggcc gcacggtgat
     3901 gcccgtcggc gatatacagg acgtcgttcg cgaacgcgtc cacgaaagat tggacgagcg
     3961 ccgcgtccgg cagtgcgcgc aacacgtgac gcacgccgcc cggtgcggta aaatcgcact
     4021 ccggcgccgc cgcgcgcgcc cgcgcgaaca ggtccgcctt cgagccgtcc tcgcggtaga
     4081 acaggtacac cagaccggcc tgcgcgccca gcgcggccat gtgacgggtg cggtcccgct
     4141 ccttgtcctc gcgcgtgagc tcgtgccgct tcacgacctt gttcgcatag tcgtcgatgt
     4201 gcacgcaggc gaccaggccg gtctgctcgc ggccatcgac ggacagggag tacagataga
     4261 gcgccggcgc gccgtcggga cgcaggcgcc cctgctcgat aaagccgcgc aggttcttct
     4321 gcgcggtatc atacacgcgg tcgtcgtaga ggtctatggt ttcgggaagg tctatctcgg
     4381 gcttggtgac atggtaaaaa cttaacgggc ggttgcgggc gatctcccgc gcctcgtcgg
     4441 aactaacgac atcgtagggc agctcggcga tctgttccgc gagtcccggc gcgggccgga
     4501 gcccccgaaa tgcctttagc cgtgccatcc taccccctgt gtttgtcgtt gagtttcagc
     4561 aacatcttga cgttcttctt gttgtctttc ttgacgctaa agccgctgcg acggagcgat
     4621 ttttcgattt tatacgatat aaaatccacc acttttttgt ccatagaatt attacccttc
     4681 ccccttgaaa tgcatgccaa ctccgcgcag cgcgcacgga ccacccgagc gcccggttcg
     4741 ggaggaaacc ccgcacgacg gaactgcggc cgccgcgccg agatcacccc gtgccgagac
     4801 gcggtcactc gcgacatacg gatgtctttt ctgatattag tgtcggccgt tggggtgcgg
     4861 gcattgagtc gttttacggg atgatccgtc cgcgggggcc cggccgccgc gggctacagc
     4921 gaaccggccg ccgaaaacgt gacgctcccc ctcgagagcg ggccggacac gtcccaggac
     4981 caggcggcag cgatcgacac gccgcggaac tggacggtgc acctgacgcc gcacacgacg
     5041 gccgtccgcc actcgtcgtc ttcgttgcga taccagccgg cgaccgcgaa cggccccgcc
     5101 atggccagat cggggtaggc ctcgaagagg agatcgatcc cggcagtgtg tttccagcgg
     5161 gaggccgtga agtcgtccga acgaccggga agggtttcgt cgaacacgta gcgccagtga
     5221 tagcgctcgg ccgaggtggc gtaccaggcg tcgaagtgcg ggacgacgaa caaccacggg
     5281 acggggacaa gcgccgtccg tcccgaagcg gcggccatgg cgtatccctt tgggcaaagg
     5341 tcgcttgcgc cgcccgaggc agagaacgca agctccgagg cgcgacgccg gtcgagcagg
     5401 agccggcggt cggcgatgcg gtagaccgcc tgcgcgcggc tgtcattgtc gacataccag
     5461 ttgccccggt agcgctcgag gccgctcatg gcctgacgga acgtgcagtc gagcgacgcg
     5521 ctcccgaagc gccaaccgta gccggcgaac gcctcgcccg cattcgacag gaactcgcgg
     5581 tccatggact cccacacatc gcgcgcatgc cgatagcgcg cgccgagcag aagccccccg
     5641 ccgtccgtct cgcgatcgcg accgtcgaat tcgaccgcgg cgatcccctg gcggagatag
     5701 ccgtcgcgga aaccgatctc cgcgcgcgag cgcacggccc cgccgccggc gggacgcgcg
     5761 tactcgatca ccggcgcata gccgtaccgg ggcccgtacc ccatgccgaa accaacggcc
     5821 ccgcgcggcg cgacgagtgc cgttatccgc aacagcacgt cggcgccatc gccgtgattc
     5881 tccgcggaga cgcgcaccga cgacaatcct agcgccgccg acgcgaagcg cgcgcgctcg
     5941 gcgacgacga tgcgattgta tatctcgccg ggctcgggga gcatgagcgc ggcgagctcc
     6001 cgggccgccg gtccgtcgac acccgcgacc gcggcctccc gcacgcgcga ctcgaccaca
     6061 tgcacctcga gcgcgccgtc gcgccgcgcc acgagccgcg ccacatggca ggtgacgtat
     6121 cccgcctcgt gatagcgccg ggtgacctcg acgccgatcg cgcgaatgtc ggcgtcgctc
     6181 aggtcgcccg tgcgcccggc gacgagctcg gccagcccga tatcgacgct cgatccgacg
     6241 atgacgatgc gcgaaatcac gaccgcgcgc gcgtccacgg cgacgagcag cgccgcgacg
     6301 gccaggagca tgcggcagcg tcccgccatc agcggtcccg gtagaggcga cggcgggcgt
     6361 tcggcggaaa atcgggcgcg aagaggccgg gcgaaagggg gatgttgatg cgcgcgtcgc
     6421 gatacaccgt cgtgctgcgg acaacgccgc gctcgccgcg cgcgcggacg tcgatccgcc
     6481 gcggataaca ggcgccctgc gtccggtcgt aatcgctgaa gacctcggtg acgatctcgc
     6541 gtccgcgctc gtcaaggagc cgccgttcca gcaccacgcc gcgccgcgaa tcgaccaaca
     6601 cctcgatccg aacccgggga tcgtctccgc gcacgacgaa gcgatgcgcc aacgtgaaga
     6661 tcccgtagag gcgccgcccg agatactcga cctcgccgcg ctcgtccaag cgccgcatga
     6721 gctcgtcggc cggtcgcgcg ccgggcgccg gcgcggatcc gcctcccccg ccgatgcgat
     6781 agagcaggtt gtcagcggga taataccagt acagggccgc gcccgtatgc agaacgcgtt
     6841 gcaccgacgg ggcggtatag tcgatccgga accgaccctg ggcgtcggca cggtagcggc
     6901 cgcggaacac ctcgggaggc gcgccgggat agcggatgtg ctgctcgatc acggcgtcga
     6961 tgctcgaaac gccggcgcac ctgccgacca gctcgcgcaa caggtcgcgc ccgtcagtcg
     7021 ccccggcggc cagcaccccc atcaacacga aaaccgtccc catgcccatc gagcgccctc
     7081 ctccctcgac gtttatatcg gcccggggag gatttcgaca ataacattat gcacgccgcg
     7141 atattttcga ttcaccccga ccccggtccg tcccctggcg atcgcccggc cggcgcgcgc
     7201 acaatccgca tccagtgcga caaaattgta cgaatgaaaa tcggcttgac ccgtttcccg
     7261 caatcggcta tgctgcgtac cgttatgaat ggccctcgtc agggcccgtc atcgatgccc
     7321 ttcggaggaa tgcatgcata tacccgatgc atccagcccg ttctggatca gcgcggtcac
     7381 ctcggagcga acctacgagc tggagtttct cgccatgaag atcctgctcg cgcggcttcg
     7441 tctcgcggta aagcaggatg caagtccgga gtcgattcag aagtgcgcac acgagctccg
     7501 cgagctgttc ctcaagtccc agcacctgcc caaggcgcag aacgatctca aacgcatgat
     7561 ccaagaggga ggcatgtcat gaacccggtt ctctatggcc tcgacgaggt ggcgcaacgc
     7621 atcacaaccg gggcgcgcct cgtactcgcg ggggacgagg cggtgctctc gaagctcccg
     7681 aagggccggt ggatcggcgg caccattcca tacttcatgg gcgcggatgg cggggtgatg
     7741 tcgaaggacc tgatccacgt gaccgagctt cccgcatccg tcgagaccgc gtcgtgtacg
     7801 acctaccgcg ccgacaccat cgcccgcgtg tacgcggacg cgccggcaaa cggcttcagc
     7861 atcatcatca tccccgcggc cagccccacg cacctctcgt tcgcgatcaa tgcgcccacc
     7921 tacgagggtt tcgcgacaag cccgctcatc ggctggatat cgggggtgca tgtggacgag
     7981 atcgggaagc gccgcgcgaa ggtgtttttc ggggaaacgg gcgaggcgct cgaggacggc
     8041 gcggtggtga tgcaggcgac cctgcccgcg ggcacctacg ccgatctgaa catcatcaac
     8101 atcttctccc agggcgacgg cgacaccatc accttccccg aggacggctt catcgtgggc
     8161 gatgcgttca tcaacgggga gaacacgaac ttcgccgact acatcgccgc gcggacgctc
     8221 gacacgcgat tgccgctggt ggccaactac tgcggcgcga tgatcaacat cagcatccag
     8281 cggatcgaca cggcgaaccg acgggtgcac ctctatgcgc cggtgttccg cgacgtgcag
     8341 taccgccacg ccgcgcccgt gggcgattac gtccgcgagt tcaccggaca cctgccgcac
     8401 gacgggatcg actccatctt cttctcgtgc aactgcatcc tcaactacct gtactccgag
     8461 ctcgagggca ggaagaccgg cgggatcacc ggtcccatca ccttcggcga gatcgcctac
     8521 cagctgctca accagacgat ggcgtacctc accatccgct cgctctagcc gcgctccgcg
     8581 gccgacgcgg cgtccgcgac gggaaagagc atctcggcca tcacgcacag cttcggcgcg
     8641 cggcccgtga ggtcgcggat atcgcggatc atgaacaaac ccttgtgctt gcggacgata
     8701 ttgtccacca ccgtgagccc cagccccgaa ccgaacttct cgtacttggc gatcgattcg
     8761 tccggcggga ggatccggaa gaagggctcc agcaccatct tctcgtaatt ttctgggatc
     8821 ccgcaatagg gcttctccga gacctcgttt ttcaccgaga gccagaagta gccctcgctc
     8881 tgccgggcga tgatgttgat gacgctcccc ggcaccgagt acttgtaggc gttgatcatg
     8941 agctcctcga cggcgagcgc gaccttctcc atgttcagat cgagcgggca ggccgcgcgc
     9001 ggctcgggat acgtgacgcc gatccggcgc gcctccatgt agggcagcac gccctcgatc
     9061 atccccggca gcgcctcgat caggtccgtc gaggcggcgc gcgagagcgg gacctcgttc
     9121 tcgatgatgt cgctgaccat ctgcaggccc tcgatcatga gccggcagaa gtcgttgttc
     9181 tcgatgatga tgtcgacgat gttcttgtcc acgaggtact tgtcgccctg gggctcggcc
     9241 gcgctcttga gcagctcgat gagcgtgacc agcgtcccga tgccggcgcc ctgcgacatc
     9301 gaggtcttga gggagtagat ggacttggtg ctcgagtagt cgcggtcggc cacgcgccgg
     9361 ctctccttgt agttgagcca ctcgatctgg ctgcggatct tgagcgacgc gctcgcgcgc
     9421 tggccctcct ccaggtcctt caggtgcttg tattcgaccg ccttccagac gatggcctgg
     9481 aacacctcgg gatcgatggg cttgatgagg taatcgtaca cgcccaggcg catcacgctg
     9541 atgatggtct ccgagaggtc gaggccggtc tgcaccagca ccacggcgtt ggggtcgcgt
     9601 tttttcagct ccttgatgaa ctgcttgccg tccatgaccg gcatcatgag gtcgacgatg
     9661 tagagcgaga aggcgccctg ccccacgcgc tcgagcccct cgcgcccgtt gcccgcaaca
     9721 acgcagggga tctgcatctt cttcaggatg cccttcagga gggcctgggt ctcctcgttg
     9781 tcctcgacga cgaggatcgg atcgcggggc ggctgcacgc ccacccgggt cggggtcaat
     9841 ttctcttccg ctgccatatc tctctccgct tgtctgggct cgggcggtat gatcgcgccg
     9901 cgggcgccgt cgttgcaact catttttccg ccgcgcggcg ctcgctcagg gcgcgatcgg
     9961 cagccgcacg gtgaaggtgc ttccctcccc cggcgcgctc ttcagggtga tggcgccgcc
    10021 atgcgcctcc acgatcttcc tggcgatgga gagccccagc ccggtcccct cggtctttcg
    10081 ggtgaggccg gtctcgacgc gggcgaactt ctcgaagacg tactccgcgt gctcggggct
    10141 gatgccgacg ccggtgtcgc tcacgtcgac ctcgagcgcg ccggacgcgg tccgcgcacg
    10201 caccgcgacc cgcccgccct cgtcggtgaa cttgacggcg ttcgacagga ggttgatgaa
    10261 cacctcctgc agcttcgcct cgtccccgag gatctgcgcg ccggcggccg cggcggcgtc
    10321 aatggagatc gcgatattcc gcttcgcggc ctgcacctcc atcatgcccc cgcactccgc
    10381 gatgatccgc gcgagatcga ccggctttcg ctcgatctcg aagcgcccgg actcgatgcg
    10441 ggatatctgc aatatctcgt tgaacacctt gagcaggtgc gtcccggaga agatgatctt
    10501 gtcgagattg gggccgtaat cgtcggggtc gtagcccatc ttcatgagct tggcgaaacc
    10561 gatgatggaa ttgaggggcg tgcgcagctc atggctcatg ttcgagagga actcggtctt
    10621 cgcgcggctc gcggccatgg cggcgagggt cgcctcacgg agctcgtcct ccgtcgcctt
    10681 gcgcagctcg atctcggcgg agaggaggcg gttcgcctcg gtgagctccg cggtgcgccg
    10741 gcgcaccagg tcctcgaggt tttcctgata ggcgcgcagc tcctcctccg cgatcttccg
    10801 ctcggcggtc acgcccagtt cgcgggcgac ggcgtcgacg agatggacct cctcgtcgtc
    10861 gaagggaacg gcagcgcggc cgtcgggtcc gtcgacatag cccgcttgga cccgcccgcg
    10921 caccgcgccc cgcaccgtta tgtcggcggc gagcatcgac gcggtctcgg cgaaggattc
    10981 cgtacggtac tccgccccgt cgtattcgat gcgggctccc gcgcgggcgc ttgcgcgcag
    11041 ggccgtccgg aggtgctctg ccgttgtgcg aaatatctcg tccagcggcc gatccgcccg
    11101 ctccaccagg cgcgcgacct tatagaggca atcgagcgcc ttgagccgcc cggtgatgat
    11161 ctcgacgaag cgctgccgct ccgcgtcctg tttcttccgc cgggtgatgt cgcggagcaa
    11221 ccccatcacg tgccatcgcc ccttgagctc cagggccgag gcggcgatct ccaccgggaa
    11281 ctcgccgccg tcgcggcgca tcaccaccgc ctcgaagacg tcgtcggcgg tcattccccg
    11341 cgcggggtca cgatacatgg agaagttccg tgagaactcg tccaacatgc gcgcgggcac
    11401 catgagctcg gggatcgggc ggcccaccgc ctcgtcggag gtgatgccga acatcgtctc
    11461 ggccgcgcgg ttccagaaga caacgcgcgc gtcctcgtcg cccatcacga tggcgtcgcg
    11521 cgccgcgccc gcgagtacgc gaaagcgctc ctcgctgtcg cgaaggcgcc gctcgatctc
    11581 cttgcggtac aatgtgatct cgaggcaggt ggcgatatcg cgctcggtga agggcttgat
    11641 gatgtagccg aagggcgccg tgatcttcgc gcgcagcagg gtcgcctcgt cggaatagga
    11701 ggtcacgtag accaccggaa cgtcgctgtg cgcggcgatg cgcgaagccg cctcgatccc
    11761 gtcgatcccg tcgcccagca tgatgtccat gagcacgata tccggacgga ggcgcgcggc
    11821 catctccacc gcggcctcgc cggtgggcgc gattccggcg accgcgtatc ccagacgcga
    11881 cagcaccccc tgcaggctgc tggccgtaat ctgctcgtct tcgacgataa gaacgctttt
    11941 cttcatggac cgagcccctt ccgcaacgct gatggcgccg gcaggcgcga tggacgtccc
    12001 ccgcgggcgt cacaccctac ggcatatcgg gctcggtgtc aattcacttt actgcttctg
    12061 cgaacgggac cgacgcgctc atcgtgcgca cggatcggcg cgcgtgacgg cgttccgcgc
    12121 tccatcatcc atacaacatg cctcgcttca tgttgataag gccctcggcc gcaagctcgc
    12181 cggcgacatc gcgaatctcg tcgcgcgtcc ggcccgtcat gcgcgcaaca tgcgccacgg
    12241 aggcgccggg ccgctcgagc acgatgcgta aaatggcgcc gcgaagctgc cggcgcgatc
    12301 cctcaaagcg cgcctgccgg gcatggtgcg cgctcgcgcg ggcgggattg ggatagcgcc
    12361 gcttgagcat cgcgccgtag tccatgagcg ccgaatacca gacgtgcgcg ccgcggcgcg
    12421 gaagcgtgcg cgcgaccagc ggcgcaatct ggccgtcgcg cacccccgat cgcccgtgga
    12481 aaaaaaagtg gatgaatacc gcgcggatat tggtctcgat gaacaccgtc gggcggtcga
    12541 aggcgaatgc cgcgatggac gccgccgtgg ccgcgccgat cccgggaagc gcgcggagtt
    12601 cctcctccgc gtcgggaacg caaccgccgt aacgcgccac gatctcccgc gccatgcggt
    12661 gaagatacgc cgcacggcga ttgtagccga gcccctgcca tgcggcgagg acctcgcgtt
    12721 gtgcggcatc ggcaagggag cggaccgtgg gaaaggacgc gatgaaggcc ggaaagcgct
    12781 ccgccacgcg cgcggtctgc gtctgctgca gcatgatctc cgacaccagg atgtgatagg
    12841 gatcgtcggt ccgccgccag gggaaatcgc gcgcgtatcg ccgatagtag gcgcgtatga
    12901 cggcgcggaa gcggcgcacg cagtccggcg gaatctccgc gcgccgcatt cccgggaagc
    12961 gccggcggaa ttcggcgctt atccgctcca ccggatagcg atcgtcgcgc ccatgcgccg
    13021 ccccgccaga tccgacggcg atcatcgcat cccggcgaga ttcgcgcgaa aggcgtcgcg
    13081 cgagacatag ttggattccc gcaccagcgc tccctcccga tagtaccgca ggtaggggat
    13141 ctgcctgttc ccccagacgt cctccttgaa ccgcatgaac tcctcgaaga cgccggaccg
    13201 gttgtactcg aagacgatca cggcgacgcc ggcctcgtcc gcagcgtcgg cgagccagtc
    13261 cttcatggcg gcccattgcg ggcaccaatc ctgcgtgagc accatcacgg tgcggggcgc
    13321 ggcgccgacg agtgccggag cgaactcccc gcgcgcgatg gcgtcccgcg cctgttcgtc
    13381 ggtcaatgtc gcgtacatga atcctccctg cgggcgatcg atgcgcacca tcgaacaact
    13441 ctagcacaac aatgaagccc gcacgtattt ttctgacgca gggcgccgcc caggatggcc
    13501 cgagggacga ttccggtacg ccaccgcgcg caatcacccg gactattgtt ttattacgcg
    13561 gtatacatcc aacgaggatg tacggggatc tgcgaggagg ccgggggcgt cgtgcggtcg
    13621 cgcgcgaagg tgttcgataa aacgtttcag tgtgaacgca tacgacgaac ggcggcattg
    13681 tggcgtcgaa gctggcgcac gaggtcctgg agctcctcgg ggtcgacgat cccccgctcg
    13741 acaaaaaagc agccgatggg tttttgcagc atatgctgtc gtcccaggat cgccttgtgc
    13801 tcgaggtcgg tcacgtaccc attgcgaagc gcgtattccc cgattttctc gttgtgatcc
    13861 cgcgcggcga gtatccgcgt gatatcctgg acagtcatga ttttccagtc gatcgcgatc
    13921 ttcccgtatg acgggcgctg tttccgctgc cagaatacgg catcggcaag cgtgttgatg
    13981 gatatccgcc gctgatagaa aaggaattgc cccagcaaaa gctccgttgc cgggacaatc
    14041 ggtttcaccg ggccggaggc ctgcgcgcga aatgagaacc ggtgcgtgat ttccgtcgtc
    14101 gatgaccggg aattgttttt ctggacaaat tccgcaagat gcaaataggc ttcattgatc
    14161 gcgcgaaaat ggcgctcaag ctcgtcgggg tcgcgcccca gcagctttgc gcggtcggga
    14221 tggttttcca tcacccggtt gcgatacgcg gacttgatgt cggacaggct caaaagccgc
    14281 aaaaagtcca ccgtgatccg ggtctgctcg gaaaacagcg tcctgcacga tcgaaaaaga
    14341 tcggtatcgg ttgattggat aatcatgttc ggaccgtcac atcgggcacg atagtatctt
    14401 ttcggttcac ccatggaaaa agtacatcgt tctttgcgga attacacttc ttttctcatg
    14461 gaagtgcaac tatttcacgg cgccgtgaaa cggcaatcat aatcgtcaat gacgcagcgc
    14521 ccacgcctcc acttcctttg gaaaccattc cagcagggag tcgattgaga cggtaggata
    14581 tcatgctcgc cccgccgcga acaatacatc catgtattcg cggctccagc agccaatccg
    14641 ggctgcttgc tcgctgtgac atcctgccgt ctcaatcggt gctattggta ataaacctac
    14701 tgaaataggc ccttagcaca atcattggcg ccaatgacat actgcgttga aaaacgggtt
    14761 gacgtaatat caaaaaacgc catagtagaa atgtcgaggc tgtgcgagat cgcatgggta
    14821 gcgcggtccc gtcacctcgc ggaatatgat ggaaacgata caaacacctc agggcgtcct
    14881 ggccatcagc gacgaagagt tcgaattctt ccgttcgatc gtgtacgaag agtccgggat
    14941 cgacctctcc gacaagaaaa aggcgcttct gcaggcgcgc ctgaccaagc gcttgcgcga
    15001 gctcaggata cgcacctacc atgaatacat cggccatctc aaaagtcatt ttcacgatga
    15061 gatcgagcac ttcatcaatt gcatcaccac caacaagacc gagtttttcc gcgagggggg
    15121 gcacttcgaa tttctgcgca cgacggtcct gcccgcctat gagcgcgaga aacggcgtcg
    15181 catacggatc tggagcgcgg ggtgctccac cggcgaggag ccgtacacca tcgccatcgt
    15241 cctgcttgat tacttcgacg gacgggccct cccggacgtg aagatcctcg cgaccgacat
    15301 cgacagccgc gtcctcgaga ccgcgaaagc ggggacctat aaagcggagt cggtgcacat
    15361 gatcgggctg gacatgctcc gacgctattt ctaccggggc accggggaaa accaggggct
    15421 cttcaaggtg aagcacgacg tccgccgcct catctctttc cgccggctca atctgctgga
    15481 cgaatcatac cccatgaaaa atacatttga catcatattc tgccggaatg ttatcattta
    15541 tttcgacaaa gacacccaga acgcgctgtt cgcgaagttc cacaggtatc tcgccgacga
    15601 cgggtatctt ttcatgggtc attccgaggc gctgatcggc cggtccagct tgtttcagct
    15661 caagacaaac gccgtgtacg agaaatcggg gaaccggtaa cggggacggc gcaggggcag
    15721 catcgacgga gcgagggcat gggaaaagta aaggtgctga tagtcgatga ttccgcggtg
    15781 atgcggaaga cgctcgagaa atcgctcgtg cgctccaagg acatcgaggt ggtgggcacg
    15841 gcgctggatc cctacatcgc cgtggacaag atcaagaagc tccaacccga cgtcatcacg
    15901 ctcgacatcg agatgccgcg catggacggg ctcaccttcc tttccaagct gatgatcgcc
    15961 acgccgatgc cggtgatcat ggtgagcgcg ctcaccgacg cgggagcgac cgtgacgatc
    16021 aaggcgctcg agctgggcgc ggtcgatttc attctcaagc cgcagctcga ggacgcggcg
    16081 ggatgggacg agttttcgcg cgaactcgtc gccaaggttc tcgcggccgg gggcgcgcac
    16141 gtgaagcgca aacgcaccga tgccccggca cccggcgcgg tgacgatctc ggtggaccag
    16201 aagttcagcg ccgacgtgat ccttccaaaa aagcgccccg atcttgcgaa ggcgcacacc
    16261 ggcaccgtca tcgccatcgg cgcctccacg ggcgggacgg aggtcatcaa cgagatcctc
    16321 atggggctcg gcgaggatgc gcccggcatc gtgatcgtgc agcacatgcc tgagaaattc
    16381 accaaggcc
//
LOCUS       DAORHF010000055        16360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2636_length_16360_cov_6.771052, whole genome shotgun sequence.
ACCESSION   DAORHF010000055 DAORHF010000000
VERSION     DAORHF010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 16360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16360
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2636_length_16360_cov_6.771052"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..2465)
                     /locus_tag="PKJ16_06750"
     CDS             complement(231..2465)
                     /locus_tag="PKJ16_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46722.1"
                     /translation="MIGTTWRRAIVPIALLAPLVFGMRLPANERSVLTIFLTANLEGR
                     FSLSGGARDADDPFLMLARNLLYARGRTPGSLYLDMGNSMTPGVLSRFSYGSLVMDFF
                     DQFDCRATLVSSRDISIGTENLDQGARGRSVRLLSSNIVANGEPLFAPSFEYVQGTRT
                     IGFVGVSSKRALVDIAEQKARNVEIDDDRESVGRAVSDLRKRGVRSIILLSGLTTAEN
                     MRLLRSFPDIHLVISGGDNRGELSGSPVSRIQLADGRAIVFLSGESGFWRLTIGVDER
                     ISVTDMRYFKLEPLPSSDPRYAQFRRRLAQWKRKYRRETNDAIADVGEADHIIDPFRI
                     ANLLRGAFGAEVAVVEPGMAQDGILRGAVRRHDVLRAIVDEHSLFTYTLTGEELTDLF
                     NDPGDLIINGFSGEKIQNYPVQDERRYKIVSTQRVYELIEDKLGLDGTFRNSWKSAGD
                     IVREDLQGRQNLLRDDFGYLERMFRSTIDFYISSFFYERLTLVRDEHITTPPGQPERS
                     YIRWGIESAVDFTVYNRYHQFILTPYIYYIKEEYEDKDKVLQKYFLMNQLRGTFVYNL
                     NLAWYTRPYHKSLVDTVVEAERNEETGRDLRPVLIRETVGTAILSKYVDGRIGVGFEK
                     WIQDPVEDPIYGLEALLTVKVSFLRYLTYSLSVDAFFSKREDVDPIYVKSQIENALAF
                     KLNSLLSISVKHKWYYLYSRVSVEEEHETEDDYYYVTKHYNEKYDYSRIMVSLDLKTD
                     FKVY"
     gene            complement(2491..3138)
                     /locus_tag="PKJ16_06755"
     CDS             complement(2491..3138)
                     /locus_tag="PKJ16_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46723.1"
                     /translation="MKRLFPCLTMACMMIGCASMEAGSPTENAAYEISLLRKNVTHLQ
                     RENEVIKNENLEYRRDARIKQAEIDRYKAEVEGLKKHHAGELAEAEQRYRTAQEQAAT
                     LERILREKIKTVEEQRAQAEARMREELKTAAEQGRRRDEEHRQSEEKLKREYADREFQ
                     YSKEANECAKRLSACEQNAAECRARIEERALRSDERPGTSGSPRDRTPPAAGVKK"
     gene            3358..4656
                     /locus_tag="PKJ16_06760"
     CDS             3358..4656
                     /locus_tag="PKJ16_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HNV46724.1"
                     /translation="MSDPRSYHRNPVPGKTAIHPTKDCKTQADLALAYTPGVAVPCLD
                     IQKNPADAYAYTNRGNLVGVISNGTAVLGLGDIGALAGKPVMEGKAVLFKRFADIDVF
                     DIEINEKDPQRLVDIIASLEPTFGGINLEDIKAPECFYVEPELQRRLSIPVFHDDQHG
                     TAIITGAALVNALEIIGKNVADIRVVVNGAGAAGIACSDFYVSLGVRKEHVIMCDSKG
                     VLYRGRVEGMNEQKNAYAVDTPARTLADAVKGADVFLGLSVAGALTPDMLKTMNNNPI
                     VFACANPDPEILPDQARAARADVIMATGRSDFRNQVNNVLGFPFIFRGALDMKATCVN
                     EDMKIAAARALARIAKEPVPDYIRTAYAGNALSFGPEYIIPTPFDHRVFFEVSSAVAE
                     AAAETGVAREAFPGREAYRERLRARYRSSIARYSDVPIEV"
     gene            complement(4936..5070)
                     /locus_tag="PKJ16_06765"
     CDS             complement(4936..5070)
                     /locus_tag="PKJ16_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46725.1"
                     /translation="MKKGMMIAVSAVLASVVVFSSCRSDQGKHKGWFKYDRLPDSPDR
                     "
     gene            complement(5067..7088)
                     /locus_tag="PKJ16_06770"
     CDS             complement(5067..7088)
                     /locus_tag="PKJ16_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV46726.1"
                     /translation="MNTIRTVCAAVIIALAVSLVVGASEESFHYYYNRALLERNNRER
                     VSYLTRALSLWSDTDGRLPKVRAYNARGNAYMDLGKYRESLGDYNAALALDPDFAPAL
                     NNRGIVHRRLNRLDDALADYNKALSRRTGSSEAYYNRGIIQYLKKDYRAAIADYGRAI
                     EADPGLAIAYNNRGVAYYLLNDIDRAITNYDRAIELDRQYEIAYTNRAIAYRKRKQLD
                     RAISDYNTAIRINPKNEMAYVNRGRTYSDKGVFKNALMDFGKAIEISPGCSIAYYYRG
                     DVFISQKRFEEAIKDYNRSIECDPNDVSARLSRGNAYLEHDRYESALRDYNKVIEQNP
                     NISDAYNNRGVAHYAMGNRTQALADFNAAIRLNPRNIRAYNNRAVLFASQGKRDRADA
                     DYATAIRIKNEYKYAQYNKGNLLLREYRPTDAMNHYNRSISVKPNFPEEFINRGIAKH
                     HLGQYREAILDYNTALALDGRLGQVYYFRGMANERLNNFDRALADYTMAISMNPRNAD
                     AYAARALLLQRMGRRDEAQRDFNRALSLDSNDARIFALRARLHLLEGRLEQALADLEQ
                     AIKLNPNDQTLKDERDRLLAMMKGDNLNIGPRAAQDSSAGSGIPVSLPVALAIGITLV
                     LAAGTGIALMRRKKTSKAAPSPRMEQPDGETVARPAPSKSKTKGKRRSA"
     gene            7231..8781
                     /locus_tag="PKJ16_06775"
     CDS             7231..8781
                     /locus_tag="PKJ16_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV46727.1"
                     /translation="MEPIHSTIEDSREGVPTLIIYDTNGKKVYLHSAVRPSREADSLR
                     EELASSPDGLLIVLGVGLGYHLHSLAGSRTPDRPAVLIERLPDIERHLEAVPGSRFLS
                     GTDGITILAGIEPDTLASTLDGIIDFSRIGSLRVVEHPASMRAFGDYYRAIRSVIEAL
                     VRKKAGDAAARRAFGYRYLKNALINCGSLGSLTPVSALFERFTGRPALVVMPGPSLDG
                     ALDEVRAYARRAVVIAADSALPVLSAAGIDVHFCVSIDPQGYTWEHCGRMPDPAPVMV
                     CSLTANPRLIRRGDALLSLNSHPIAQLIDQLCPDAIGSIDSTTGNVAGDAVYFAHLLG
                     CAPIGIVGFDSCFSRYRIYARGSAYQNRFARLFHDRFQPIETQNLRYIRVSSRAYREQ
                     GRFTRRSFTAYNATLARFIAARRIGGIAHVNPDGLPIEGVPAADLAAIMEHAPPVTDD
                     LRALIAGCIASAPSLADLVDVAAIRRALVVPENADAVLRASLDDKTLVKRGPAITGIM
                     TRLLGGGR"
     gene            8778..9374
                     /gene="coaE"
                     /locus_tag="PKJ16_06780"
     CDS             8778..9374
                     /gene="coaE"
                     /locus_tag="PKJ16_06780"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HNV46728.1"
                     /translation="MNIGVTGIFASGKGTVCEMFRALGARVIDTDILAREVVAPGTEG
                     LNRLVAAFGTDILTPSGELDRRGLALRVFPDPAHVQQVNAIVHPLVLSRMLEIVGEAP
                     DSIYMINTPLLYESGFDRYMHKNIVVTADTEQAISRGMRRDRLTAEEIESRLKHQIPL
                     TQKMHRADYVIDNSSSLENTRRQVIEIWNSLTEKPRGE"
     gene            9338..10204
                     /locus_tag="PKJ16_06785"
     CDS             9338..10204
                     /locus_tag="PKJ16_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV46729.1"
                     /translation="MEQFDGKAPRGVKEKSVYQLHLDTPRIIIISSVIIGVIIISVLV
                     GMNLKKADGTDGDIAKKDGLELPLSNAEKELSMLANKVPGMGETPPAAKPGELSDKDK
                     LFQKDPAGAIIPGGPAKPDGAAHPEKEAAPSGDILTKDNIKEIIPPVREASLDKPKKH
                     KLAKEAKGKKKDGEAARMSAGTKEREKKAKKNKVVEVASKTKDADDPAPRRHGFMIQI
                     GSYDNRTKADAEKSRLEKMNYDAFVDKTMKNGKRYFRVRVGPIASKRNAINTLNDLQD
                     NTRYRDSFMVKE"
     gene            complement(10293..11015)
                     /locus_tag="PKJ16_06790"
     CDS             complement(10293..11015)
                     /locus_tag="PKJ16_06790"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV46730.1"
                     /translation="MTAKPNVPVPPEAAEIQRTITGRLRTLLARAHDGADMTAIARGY
                     RAVLRLYDEYQGRVIAASGLSMKCHAGCVSCCCHWVDDVYSFEADMIAAAVRRMSHRR
                     RESVIAACRESMEEYERVRAASAVRLESGEYQEIAGEVDFEEIVLTGFYQLHSRCPFL
                     DEDDRCAIYALRPLTCRAYINLGDPAACPPENILDEEESTYILDLDGESNRLLDDLSA
                     RVARHPGDTGLRSLVLKCLEDR"
     gene            complement(11012..11506)
                     /locus_tag="PKJ16_06795"
     CDS             complement(11012..11506)
                     /locus_tag="PKJ16_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HNV46731.1"
                     /translation="MDIRGSIAPHRRRRPVATARRMMKTFYVSSELFETLAQRLLGEV
                     RADGTRYDGVLCPLRGGYWLSRAMARGLGLPIYDIEISSYVEHERKSFTFGRRPDLAD
                     GTYLLCDDIYDSGNTINMIISLYPGSRFDVVCLVTKRDVGIRSGMAVEPDVWVEYFWE
                     VGRA"
     gene            complement(11520..12158)
                     /locus_tag="PKJ16_06800"
     CDS             complement(11520..12158)
                     /locus_tag="PKJ16_06800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV46732.1"
                     /translation="MDGIRKSSLGMLLASLFSISCCSAERSANHFNALAVEPGAKPDE
                     VVRALGIAPGMAIADIGAGGGYFTVRFARDTGPAGVVFAVDINREFLDIISQTAASEQ
                     LRNVKTVHATPDDPGLPAHGVDLIFMRNVFHHIEHPVAYFRRLAGCLRPGGRIAIIDY
                     RPGSLRTLFHSVPEERIITILREAGYRIAARHEFLERQSFTVFMVDGNTVRR"
     gene            complement(12253..12705)
                     /locus_tag="PKJ16_06805"
     CDS             complement(12253..12705)
                     /locus_tag="PKJ16_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV46733.1"
                     /translation="MSFVKILPIVAVVAVVGFMSLQGCRHRAPFCKADFADKALARID
                     ARIEKLNLNEAQRQKYDEIKASAKRDFASFTEKRKKVHEEIGAELNERTPDARKIARI
                     ALTAHKERPQKFEEYVTKLVELYEMLDDGQKAQVVDEMRKHHDRMQCR"
     gene            12886..13587
                     /locus_tag="PKJ16_06810"
     CDS             12886..13587
                     /locus_tag="PKJ16_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV46734.1"
                     /translation="MDRKILIIDDDAKLQDLLRAYLSDNGFAVASLFDGNEALSAIEK
                     EGPDVVLLDIMLPGSNGLDVLRAVRATSSIPVIMLTAKGDDTDRIVGLELGADDYLPK
                     PFNPRELLARIRAVMRRAPAPGVTPAHDDVAIIRAGGLVLNPALLALERGAERTALSK
                     TEYRIMETLMRNPDRVFSRDDIMNAARGRDFIAFERSIDVHISKLRAKVESLTGERDR
                     IKTVWGTGYLFRGDT"
     gene            13584..14957
                     /locus_tag="PKJ16_06815"
     CDS             13584..14957
                     /locus_tag="PKJ16_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV46735.1"
                     /translation="MRIRQLYVKMFLAFIGLFLVAQFLVFGMFRHVTGRPLHREMGGY
                     LESSVALVRELVEHKVREHPDAFTREVAALRDFVVRLGAGFQSLVWIETAAGRVVYRS
                     SPDAPPPSIPRDLVDRGSYQYHLSMRLGSIVYLRIRVSPPGSPSATVHVFHRRPRPFM
                     HDTHFLLGLIGICVLMALLLYPLTRYITRPLKRLTHSAHRIAGGDFDQVVAVPSNDEI
                     GELAAAFNTMAERISHMIRGTRELTANVSHELRSPLARMRVALELLDAKVRDARAAGA
                     GELIASIEAEIVDMDRLIDRILELAKLDLREADTPIDAIDLDAGLNAIIAKFMPTIER
                     RRLALTLTGAGGAIVRGVPDDIATALGNLVDNAVKYSPEGGAVDIAVTQSDGVVSIAV
                     ANASPPLADDELAGIFEPFVRLRPTGAPGHGLGLAIAKRIVERHGGSIRAERSVNGIR
                     ITITLPA"
     gene            complement(15034..15276)
                     /locus_tag="PKJ16_06820"
     CDS             complement(15034..15276)
                     /locus_tag="PKJ16_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46736.1"
                     /translation="MKKMLVVLFVLTVFGGAFYCATSPKVLACRGACSAARDKCIAPE
                     EKKVREKGKMVTKKVEKTAAEKAACEVSYTACYNKC"
     gene            complement(15364..15816)
                     /locus_tag="PKJ16_06825"
     CDS             complement(15364..15816)
                     /locus_tag="PKJ16_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV46737.1"
                     /translation="MNDTTRCEMPDANPPGEEITALLRRAKTIAIVGLSDKPDRDSHR
                     VAAYLQARGYRIIPVNPVKAEILGEKCYPDLRSIPEAIDIVDIFRAVEAIPGIVDEAI
                     AVRAGAVWMQLGLAHRESAERARAAGIAVVQSKCIKIEHARVFGAAAP"
     gene            complement(15867..>16360)
                     /locus_tag="PKJ16_06830"
     CDS             complement(15867..>16360)
                     /locus_tag="PKJ16_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46738.1"
                     /translation="APVSNSLRIECEYCGVPMNSTGSEWIITDVMSVAEYEEYLRTNR
                     DAVSYSVGARLVDTLHDVRDFAFNNVMVMIAADGVYADEERRFAEELAKKWGYDITRL
                     EPFFAMARSGSLVIRMPEDMKKRRKIYALMKKAAEADANVSPEEKQLLESIGREYGIG
                     EAA"
BASE COUNT         3015 a         5211 c         4925 g         3209 t
ORIGIN      
        1 gcgtcgaaac cgagcgcagc gccgcgagga tcgccagatg gagcgcgatc gagagcgcca
       61 gccacaatag aaaacgccgc cgggcgggcg tttggaggat gtccggcgat tgcgtatatg
      121 ttctcatcgt aagcggcgcc tgttaaacct tcgtgcagca ttcgagcggt tactctacga
      181 acccggatat ccgtgaaggt atcgctggca agcagaaaac cgctgatcgg tcagtacacc
      241 ttgaaatccg tcttcaggtc cagcgagacc atgatgcgcg agtaatcgta cttttcattg
      301 tagtgcttcg tgacatagta gtagtcgtcc tcggtttcat gctcttcctc gacactcacg
      361 cgggagtaca gatagtacca cttgtgtttt acggaaatgc tgagcaggga attgagtttg
      421 aaggcgaggg cgttctcgat ctggcttttt acgtaaatcg ggtctacgtc ctcgcgcttt
      481 gaaaaaaacg catcgaccga gagtgaatag gtcaggtagc gcaaaaatga caccttcacc
      541 gtgagcagcg cctcgaggcc gtagatggga tcctccaccg ggtcctggat ccatttttcg
      601 aatccgaccc cgatgcgacc gtcgacatat tttgaaagaa tggctgttcc caccgtttct
      661 cgaataagca ccggtcgtag atcgcgtccg gtttcctcat tgcgttccgc ttcgaccacg
      721 gtgtcgacga gcgatttatg gtacggccgg gtataccatg cgagattgag attgtacacg
      781 aaggtcccgc gcagctgatt cataagaaaa tacttctgca gcaccttgtc cttgtcctcg
      841 tactcctcct tgatgtaata gatgtacggt gtgagtatga actggtggta acggttgtac
      901 acggtaaaat cgaccgcgct ctcgatcccc cagcggatgt acgaacgctc gggctggccg
      961 ggcggggttg tgatatgttc gtcgcgcacc aacgtgaggc gttcgtagaa aaagctggat
     1021 atgtaaaagt cgatcgtcga acgaaacatg cgttccaggt atccgaaatc atcccgaaga
     1081 agattctggc ggccctgcaa gtcttcccgg acgatatcgc cggcgctttt ccagctgttc
     1141 cgaaatgtcc cgtcgagtcc cagcttgtcc tcgatgagct cgtagacccg ctgtgtcgaa
     1201 acgattttgt atcgtcgctc gtcctgcacg gggtagttct ggattttctc accgctgaag
     1261 ccgttgatga tgagatcgcc aggatcatta aaaaggtccg ttagttcctc gccggtgagc
     1321 gtataggtga acaggctgtg ctcgtcaacg atcgcacgca acacgtcgtg ccgtctcacc
     1381 gcgccgcgca gaatgccgtc ctgcgccatc ccgggttcca cgacggccac ctcggccccg
     1441 aacgcgccgc gcaggaggtt cgcgatccgg aacgggtcga tgatatgatc cgcctcaccg
     1501 acatccgcga tcgcatcgtt ggtctcccgg cggtattttc gcttccattg cgcaagccgt
     1561 cgtcggaact gcgcataccg cgggtcggac gacggcaacg gttccagttt aaaataccgc
     1621 atgtccgtga cggagatccg ttcatcaacg ccaatggtga gccgccaaaa cccggattct
     1681 ccggatagaa aaacaatggc gcggccgtcc gcgagttgga tccgcgacac cgggcttccg
     1741 gaaagctccc cgcgattgtc tcctccgctg atgaccaggt gaatgtcggg aaaggagcgc
     1801 aacaaccgca tgttctctgc ggttgtgagc cccgagagca ggatgatgga gcggacccca
     1861 cgctttctca gatctgaaac ggcacgcccg acggactccc gatcgtcatc gatctccaca
     1921 ttgcgcgcct tttgctcggc gatatcgacg agcgctcgtt tcgacgatac gccgacgaag
     1981 ccgatggtgc gcgttccctg gacgtattcg aacgacggcg cgaacagcgg ttcgccgtta
     2041 gcgacgatgt tggaggagag cagacgtacg gaacgaccac gcgcgccctg atcgaggttt
     2101 tcggttccga tgctgatatc gcgcgacgag acgagggtgg cgcgacagtc gaactggtcg
     2161 aaaaaatcca tgaccagcga gccgtagctg aaacgcgaga ggacgcccgg tgtcatcgag
     2221 tttcccatgt cgagatacag gcttcccggt gtcctgccgc gcgcgtagag caggttccgc
     2281 gcgagcatga ggaacgggtc gtctgcgtcg cgggcgccgc cggacagtga gaagcgcccc
     2341 tccaggtttg cggtcagaaa gatcgtgagc acggaccgtt cgttggcggg caggcgcata
     2401 ccgaacacga gcggcgccag gagcgcgatc ggcacaatgg cgcggcgcca ggttgttccg
     2461 atcatccgca cgcggccgct cccgatccgg tcattttttc actccggccg cagggggcgt
     2521 ccggtcgcgg ggcgatccgc tggtccccgg ccgttcatcg gaccgcagcg cacgctcctc
     2581 gatccgcgcg cggcactcag cggcgttttg ctcgcacgcg gagaggcgct tcgcgcactc
     2641 gttcgcctcc ttgctgtatt gaaactcgcg atcggcatac tcgcgcttca gtttctcctc
     2701 gctctggcga tgctcctcgt cgcggcggcg gccctgctcc gctgccgtct tgagctcctc
     2761 gcgcattctc gcttcggcct gcgcgcgctg ctcctcgacc gtcttgatct tctcgcgcag
     2821 gatgcgctcg agcgtggcgg cctgctcctg tgcggtccga taccgctgtt cggcctcggc
     2881 gagctcgccg gcatggtgct tcttgagccc ctcgacctcc gccttatacc ggtcgatctc
     2941 cgcctgtttg atccgcgcat cgcgccgata ctcgaggttc tcgtttttga tgacctcgtt
     3001 ctctcgttgc aggtgcgtca cattttttcg gagcaggctg atttcgtacg cggcgttttc
     3061 cgtcggggac ccagcttcca tcgacgcgca gccgatcatc atacacgcca tcgtgagaca
     3121 ggggaacaat cgcttcatgg gaacgcacct cgcttccggc ggattggaac cgacgtatcg
     3181 ttatcagctg atgcgcgcga acgcattttg caagtatttt acggtcaatc cgcgcgataa
     3241 tcgacgccgc tgcccgttgg catcccatca tattatcatt gacgaaaaac gcacgcatgt
     3301 cgtttgtaca tgcgcgcacc ggcgccatcg cccatcgatt cacaaaggag tgcaccaatg
     3361 tccgatccac gctcgtacca cagaaacccc gtcccgggaa aaaccgccat ccacccgacc
     3421 aaggactgca agacccaggc cgacctcgcg ctggcctata cgcccggcgt ggccgtccca
     3481 tgcctcgata tccagaagaa ccccgccgac gcatacgcgt ataccaaccg ggggaatctc
     3541 gtgggcgtca tctccaacgg cacggcggtg ctggggctgg gcgacatcgg cgcgcttgcc
     3601 ggcaagcccg tgatggaggg caaggcggtc ctcttcaagc gcttcgccga tatcgacgtg
     3661 ttcgatatcg agatcaacga gaaggacccg cagcgcctcg tcgacatcat cgcctcgctt
     3721 gagccgacct tcggcggcat caatctcgag gacataaaag cgccggagtg tttttacgtc
     3781 gagcccgagc tgcagcggcg gctctccatc cccgtcttcc acgacgacca gcacgggacc
     3841 gcgatcatca ccggggcggc cttggtgaac gcgctcgaga tcatcggtaa aaacgtcgcg
     3901 gacattcgcg tggtcgtcaa cggcgccggg gcggcgggca tcgcctgctc agatttttac
     3961 gtttcactcg gcgtgagaaa agagcatgtt atcatgtgcg attccaaggg ggtgctctac
     4021 cgcgggcgcg tcgagggaat gaacgagcag aaaaacgctt atgccgtcga tacgccggcg
     4081 cgcacccttg ccgacgccgt gaagggcgcg gacgtctttc tggggctttc ggtcgccggg
     4141 gcgctcacgc ccgacatgct gaaaacgatg aacaacaatc ccatcgtctt cgcctgcgcg
     4201 aatcccgatc cggagatcct gcccgaccag gcccgcgcgg cccgcgcgga cgtgatcatg
     4261 gccacggggc gctccgactt ccgcaaccag gtgaacaatg tgctgggctt ccccttcatc
     4321 ttccgcggcg cgcttgacat gaaggccacc tgcgtgaacg aggacatgaa gatcgccgcc
     4381 gcgcgcgccc tcgcccgcat cgcgaaggag ccggtgcccg actacatccg gacggcctat
     4441 gctggaaacg cgctgtcatt cgggccggag tacatcatcc ccacgccctt cgaccatcgc
     4501 gtgttcttcg aggtatcgtc ggccgttgcc gaggcggctg cggaaaccgg cgtcgcgcgc
     4561 gaggcctttc cgggacgcga ggcctatcgg gagcgcctcc gcgcgcgcta tcgaagcagc
     4621 atcgcccgct acagcgacgt cccgatcgag gtctagcgac gcaccgcgta cgcgtcgctc
     4681 atccggctct gcgtcggtcc gtgatcattc ggcgtgatgc aacacggggt ttcatttcat
     4741 tgcgagaagc gatagcgacg aagcacgctg ttctcttcat cgggagatca ctttgctagt
     4801 ctgatatccg tgtcctgcga gtttaaccgt accgaacgct tcgaaacgcg cgatcccatt
     4861 acaccattct gtatagtttc agtgtgattc gaagcgtatt cgattgagcg tacgtaaggg
     4921 cggatcggcg ccggactagc gatccgggct gtccggtagg cgatcgtatt tgaaccagcc
     4981 cttatgcttg ccctggtcgc tgcggcagga tgaaaaaaca acgaccgacg ccagaacggc
     5041 acttacggca atcatcatac cctttttcat gccgacctcc gttttccctt cgtttttgat
     5101 ttcgatggcg cagggcgtgc gaccgtctcg ccatcgggtt gctccatacg cggcgatggt
     5161 gccgcctttg atgttttttt tcttcgcatg agcgcaatac cggttccagc cgcgaggacg
     5221 agcgtgatcc cgatcgcgag ggcaaccggg agcgatacgg ggatccccga tccggcgctg
     5281 ctgtcctggg ccgcccgggg accgatgttg aggttatcgc ccttcatcat ggcgagcagc
     5341 cggtcccgct cgtccttgag ggtttgatcg ttcggattga gtttgatcgc ctgctcgaga
     5401 tcggctagcg cttgttccag ccgcccttcg agcagatgaa gccgtgcgcg aagcgcgaag
     5461 atgcgcgcat cgttcgaatc gagcgacagg gcgcgattga aatcgcgctg ggcctcatcg
     5521 cgcctcccca tgcgttgcag gagcagcgcc cgcgcggcgt atgcgtcggc gttccgcgga
     5581 ttcatcgata tggccatggt gtaatcggcc agcgcgcggt cgaaattgtt cagccgctcg
     5641 ttcgccatgc cccgaaaata gtatacctgg ccaagacggc cgtcgagcgc aagggcggtg
     5701 ttgtagtcca ggatggcctc ccggtactgg cccagatgat gcttcgcgat ccctcgattg
     5761 ataaactcct cggggaagtt cggtttcacc gagatggacc gattataatg gttcatcgcg
     5821 tccgtcgggc ggtattcccg cagaagaagg tttcccttgt tgtattgcgc gtacttgtat
     5881 tcgtttttga tccgaatcgc cgtcgcgtaa tcggcatcgg cccggtcccg cttgccctgc
     5941 gaggcgaaca gcacggcgcg gttgttatag gcgcggatat tgcgcgggtt cagcctgatc
     6001 gcggcgttga agtcggccag cgcctgcgtc cggttcccca tcgcatagtg cgccacgccc
     6061 cggttgttgt acgcgtcgga gatgttcggg ttctgctcga tgaccttgtt gtagtccctg
     6121 agcgcgctct cgtagcggtc gtgctccaga taggcgttcc cgcggctcaa ccgcgccgaa
     6181 acgtcattgg ggtcgcactc gatggagcgg ttgtagtcct tgatggcctc ctcgaagcgc
     6241 ttctggctga tgaacacgtc cccccggtaa taatacgcga tggagcaccc gggcgatatc
     6301 tcgatggcct tgccgaagtc catgagcgcg ttcttgaaca cgcccttgtc cgagtaggtg
     6361 cgcccccggt tcacataggc catctcgttt ttcgggttga tcctgatcgc cgtgttgtaa
     6421 tcgctgatgg cgcggtcgag ctgctttcgc ttccgatagg cgatggcgcg attggtgtag
     6481 gcaatctcgt actgccggtc gagctcgatg gcgcggtcgt agttcgtgat ggcgcgatcg
     6541 atatcgttca ggagatagta ggcgaccccg cgattgttat acgcgatggc cagtcccgga
     6601 tcggcctcga tcgcccggcc ataatccgcg atggccgcgc ggtagtcctt tttcaggtac
     6661 tggatgatgc cgcgattgta gtacgcctcg ctcgaaccgg tgcggcgcga gagcgccttg
     6721 ttgtagtcgg cgagcgcatc atcgagccga ttgagccggc gatgcacgat cccccggttg
     6781 ttgagcgccg gggcgaaatc gggatcgagc gccagggcgg cattgtaatc gcccagcgac
     6841 tcgcggtatt tgcccaggtc catgtacgcg tttccgcggg cattataggc gcgcaccttg
     6901 gggagccgcc cgtccgtatc gctccacagc gacagcgccc gcgtgagata ggagacgcgt
     6961 tcgcggttgt ttcgctcgag cagggcccgg ttgtagtagt agtgaaacga ttcctcggag
     7021 gcgcccacga ccagcgacac ggcaagggcg atgatgaccg ccgcacatac ggttcgtatc
     7081 gtattcatga ggcaactccg ttctggaata tatctttact ccccggcccg tctctggtac
     7141 tgtgagcgcg cgcggatcgc ccgactgcta ctagttggcc atccgtcgtt aaattatcaa
     7201 tagtttttta gcgagcactg cattttaccg atggaaccga tccactcgac gatcgaggac
     7261 tcccgcgagg gcgtcccgac acttattatt tacgacacaa acggcaaaaa ggtttatctt
     7321 cacagcgccg tgcgtccgtc gcgcgaggcc gactctctgc gggaggagct cgcgtcttcc
     7381 ccggacggtc ttctcatcgt cctcggcgtg ggattgggat accatctcca ttcgctggcc
     7441 gggagccgca ctcccgatcg tcccgcggtc ctcatcgagc ggctccccga catcgagcga
     7501 caccttgagg ccgtccccgg ctcgcgcttt ctctccggta cggacggcat caccattttg
     7561 gccgggatcg agcccgacac gctcgcatcc acgctggacg ggatcatcga tttctcgcgc
     7621 atcggctcgc tcagggtcgt ggagcatccg gcctcgatgc gcgccttcgg agactactat
     7681 cgcgcgatca ggagcgtcat cgaggcgctg gtgcgtaaaa aagcgggcga tgccgccgcg
     7741 cggcgcgcct tcggctaccg ctatctcaag aacgcgctta tcaactgcgg gagccttggc
     7801 tcgctcacgc cggtctcggc gctcttcgag cgcttcacgg gacgtcccgc gctcgtcgtg
     7861 atgcccggcc cgtcgctcga cggcgcgctc gatgaagtgc gcgcatacgc gcggcgcgcc
     7921 gtcgtcatcg ccgccgactc cgccctgccg gtcctttccg ccgccggcat cgatgtccac
     7981 ttctgcgtat ccatcgatcc gcagggctac acctgggagc actgcgggcg catgccggac
     8041 cctgcgccgg tcatggtctg ctcgctcacg gccaatccgc gcctgatccg ccgcggcgac
     8101 gcactcctgt cgctcaactc gcacccgatc gcgcagctta tcgatcagct gtgtcccgac
     8161 gccatcggca gcatcgattc gacgaccggg aacgtcgcgg gcgatgcggt ctactttgcg
     8221 cacctcctgg gatgcgcgcc catcgggatc gtcgggttcg attcgtgctt ttcccggtac
     8281 cggatctatg cgcgggggag cgcctatcaa aaccgctttg cgcggttgtt ccacgaccgc
     8341 ttccagccca tagagacgca gaaccttcgt tatatccggg tatcgtcgcg cgcctaccgc
     8401 gagcagggcc gcttcacccg ccgctccttt acggcctata acgccacgct cgcgcgcttc
     8461 atcgccgcgc gccgcatcgg cggtatcgca catgtcaacc ctgacggatt gccgatcgag
     8521 ggcgtcccgg ccgcggatct cgcggcgatc atggagcacg ccccgcccgt cacggacgac
     8581 ctgcgcgcgc tcatcgccgg ttgcatcgct agcgcgccgt cgcttgccga tctggtcgat
     8641 gtcgcggcca tccgccgtgc gctcgtggtc ccggagaacg cggacgcggt gctgcgcgcc
     8701 tcgctggacg ataaaacgct tgtcaaacga ggcccggcga ttaccgggat catgacgcgc
     8761 ctgctgggag gtggacgatg aacatcgggg ttaccggaat attcgcgtcg ggcaagggaa
     8821 cggtctgcga gatgtttcgc gcgctggggg cgcgcgtcat cgacaccgac atcctcgccc
     8881 gtgaggtggt cgcgccgggc accgaggggc tcaatcgcct ggttgcggcc ttcgggaccg
     8941 acatcctcac gccgtcgggg gagctcgacc gccgggggct cgcgctgcgc gtgttcccgg
     9001 accccgcgca cgttcagcag gtcaacgcca tcgtccaccc gctggtgctg tcgcgcatgc
     9061 tggagatcgt cggggaggcg cccgactcga tctacatgat caacacgccc ctcctgtacg
     9121 agtccggctt cgaccgctac atgcacaaga atatcgtggt cacggccgac accgagcagg
     9181 cgatctcccg gggcatgcgc cgcgaccgcc tcaccgccga ggagatcgaa tcgcgcctga
     9241 aacaccaaat tccgctcacc caaaagatgc accgtgccga ttatgtaatc gacaactcat
     9301 cgtcgcttga aaacacgaga aggcaggtca tcgagatatg gaacagtttg acggaaaagc
     9361 cccgcgggga gtgaaggaga agagcgtcta ccagctccac ctggacaccc cccgcatcat
     9421 catcatctcg tcggtcatca tcggcgtcat cattatctcc gtgctggttg ggatgaacct
     9481 caagaaggcc gacgggaccg acggcgatat cgccaaaaag gacggcctgg agcttccgct
     9541 ctccaatgcg gagaaagagc tctccatgct cgccaacaag gtcccgggga tgggcgagac
     9601 cccgccggcc gcgaagccgg gcgagctttc cgacaaggac aagctgttcc agaaggaccc
     9661 ggccggcgcg atcataccgg gcggcccggc aaaaccggac ggcgccgcgc acccggagaa
     9721 ggaggccgcc ccctccggcg acatcctgac caaggacaac atcaaggaaa tcataccgcc
     9781 ggtgcgcgag gcatccctcg acaagccgaa gaagcacaag ctcgcgaagg aagcgaaggg
     9841 caagaagaag gacggcgagg ccgcccgcat gagcgcgggg acgaaggagc gcgaaaagaa
     9901 ggcgaaaaag aacaaggtgg tcgaggtcgc ctcgaagacg aaggacgcgg atgatccggc
     9961 gccccggcgg cacggcttca tgattcagat cggatcatac gacaaccgga cgaaggccga
    10021 cgcggaaaag tcgcgcctcg aaaagatgaa ctacgacgcc ttcgtcgaca agacgatgaa
    10081 aaacggcaag cggtactttc gggtgcgcgt gggaccgatc gcctcgaagc ggaacgccat
    10141 taacacgctc aacgacctgc aggacaatac gcgctatcgg gacagtttca tggtgaagga
    10201 ataatcgaag cgagatacac cctcctatgt gagcgcgccc ggataccacc gggcgctttt
    10261 ttcgccatcc gccgtgcacc aacgcgaatg gactaccggt cctcgaggca cttcaatacc
    10321 agcgagcgga gcccggtatc gccgggatga cgcgcaacgc gcgcggagag atcgtccagc
    10381 agccggtttg actcgccgtc gagatcaagg atataggtcg attcctcctc gtcgaggatg
    10441 ttctccggcg ggcaggccgc ggggtcgccc aggttgatat aggcgcggca ggtgagcggc
    10501 cgcagggcgt agatggcaca gcgatcgtcc tcgtccaaga aggggcagcg gctgtggagt
    10561 tgatagaagc cggtgagaac gatctcctcg aaatcgacct cgcccgcgat ctcctggtac
    10621 tcgccgctct cgagcctcac ggcggatgcg gcccggacgc gctcgtattc ctccatcgat
    10681 tcacggcagg ccgcaatcac cgactcccgg cgacgatgcg acatccgccg caccgccgcg
    10741 gcgatcatgt ccgcctcgaa cgaatagacg tcatcgaccc agtggcagca gcagctcacg
    10801 cagccggcat ggcacttcat cgacaggccc gacgcggcga tcacccggcc ctggtattcg
    10861 tcgtagagtc gcagcaccgc gcgatatccc cgcgcaatcg ccgtcatatc cgcaccgtca
    10921 tgcgcgcgcg cgagcaaggt gcgcaggcgt ccggttatcg tccgctggat ctcggccgcc
    10981 tcgggcggca ccgggacatt cggtttcgcg gtcatgcgcg gcccacctcc cagaagtact
    11041 cgacccagac gtccggttcg acggccatcc cgctcctgat gccgacgtcg cgcttggtca
    11101 ccaggcacac aacatcgaac cgcgagccag gatagagcga gataatcatg ttgatggtgt
    11161 tgccggagtc gtagatatcg tcgcagagaa gatacgtccc gtccgcgaga tcgggccgac
    11221 ggccgaacgt gaacgatttc ctctcgtgct ccacatacga tgatatctct atatcataga
    11281 tcggaaggcc caggccgcgc gccatggcgc gcgagagcca gtacccgccg cgaagcgggc
    11341 agagcacgcc gtcatagcgc gtgccgtcgg cccgcacctc gccgagcagg cgttgcgcga
    11401 gcgtctcgaa cagctccgat gacacgtaga aggtcttcat catacggcgt gcggtcgcga
    11461 cgggccgccg ccgtcgatgg ggagcgatgg agccgcggat gtccacgata ttttttcagc
    11521 tatcggcgca cggtgttacc atcgaccata aaaacggtaa acgactgcct ctcgagaaat
    11581 tcgtgccgcg cagctatcct ataccctgcc tcgcgcagta tcgtgatgat ccgctcctcg
    11641 ggaacgctgt gaaagagcgt gcgaagcgat cccggccggt aatcgatgat ggcgatgcgc
    11701 ccgccgggcc tcaggcaacc ggcgagcctc cgaaaatagg cgaccgggtg ctcgatatgg
    11761 tgaaacacat tccgcatgaa gatgagatcc acaccgtgcg cggggagccc cggatcatcc
    11821 ggtgtcgcat gcacggtttt cacgttccgc agctgctcgc tcgccgccgt ttgtgaaatg
    11881 atgtcgagga actcccggtt gatgtcgacc gcgaagacga cgcccgccgg cccggtgtcc
    11941 cgcgcgaagc gaacggtaaa atacccgccg cccgcaccga tgtccgcgat cgccatgccc
    12001 ggtgcgatac caagcgcccg aacgacctcg tccggcttgg cgcccggctc gactgcaagc
    12061 gcattgaagt ggttggcgga acgctcggcc gaacaacatg agatggaaaa caatgacgcc
    12121 aggagcattc ccaatgatga ttttcgtatt ccgtccattc cgtttcctcc agggttactg
    12181 cgccccctcc gcctgacggc ggaagaggcg catcgtgtac aaacaatgca gtgacgcgtt
    12241 caaggaattc cgctaccggc actgcatgcg atcgtgatgc ttccgcatct catcgaccac
    12301 ctgcgccttc tggccgtcat cgagcatctc gtagagctcg acgagtttcg tgacgtactc
    12361 ctcgaacttc tgcggacgtt ctttgtgcgc cgtgagggcg atacgcgcga tctttcgcgc
    12421 gtcgggggtg cgctcattca gctcggcgcc gatttcctcg tgcaccttct ttcgtttttc
    12481 ggtgaacgaa gcgaaatccc gcttcgcgga cgccttgatc tcatcgtact tctgtcgctg
    12541 ggcctcgttc aggttgagct tctcgatccg cgcgtcgatg cgcgcgagcg ctttgtccgc
    12601 gaagtcggcc ttgcagaacg gcgcccgatg gcggcagccc tgcagcgaca tgaaccctac
    12661 gacggcgacg accgcgacga tcggcagaat ttttacaaat gacatgatac gcctcctcat
    12721 tttaacttga aatatttgat gcgccggaca ataccgttgt gcatcatcat gctggaaatg
    12781 tacgcaccgg gtgtgaaaaa agtttttccc gggcgtaaat tccgcggggt atatgtaaaa
    12841 aaagtgtaaa cgccatcgga ctatttttac aatccggaaa ggaccatgga tcggaaaatt
    12901 cttatcattg atgatgatgc gaagttgcag gacctgctcc gcgcatatct gtccgacaac
    12961 ggatttgcgg tcgcatcgct gttcgacgga aacgaggcgc tatccgccat cgagaaggag
    13021 ggaccggacg tggtgctgct ggacatcatg ctgccgggca gtaacggcct ggacgttctc
    13081 cgcgccgttc gcgcaacgag ctcgattccg gtgattatgc tcaccgcgaa gggagacgac
    13141 accgaccgca tcgtggggct ggagctcggc gcggacgatt atctccccaa gcccttcaat
    13201 ccgcgggagc tcttggcgcg cattcgagcc gtgatgcgtc gcgcgccggc gccgggcgtc
    13261 acgcccgctc acgacgacgt cgcgatcatc cgggcgggcg ggctggtgct caatcccgcg
    13321 ctcctggccc tcgaacgggg agcggagcgg acggcgcttt cgaaaacgga atatcgaatc
    13381 atggaaacgc tcatgcgcaa cccggaccgc gtgttctcac gcgacgacat catgaacgcc
    13441 gcgcgcggcc gcgatttcat cgcatttgag cgcagcatcg acgtgcacat cagcaagctg
    13501 cgcgccaagg tcgagtcgct caccggcgag cgcgaccgca tcaagacggt gtggggcacc
    13561 ggctatctgt tccggggcga cacatgagaa tccgccagct gtatgtgaag atgttcctcg
    13621 ccttcatcgg gctgttcctg gtggcgcagt tcctcgtctt cggcatgttc cgccacgtca
    13681 cggggcggcc ccttcaccgc gagatggggg gatatctgga atcgtcggtc gcactggtcc
    13741 gcgagctggt cgagcataaa gttcgcgaac accccgatgc gttcacgcgc gaggttgcgg
    13801 cccttcgcga tttcgtggtg cgccttggcg cgggctttca gtcgctcgtc tggatcgaaa
    13861 cggccgccgg ccgcgtcgtt tatcgctcgt cgcccgatgc gccgccgccg tcgatcccgc
    13921 gcgacctcgt cgatcgcggg agctatcagt accatctctc catgcggctc ggttcgatcg
    13981 tctacctgcg catccgcgta tcgcctccgg gatcaccgag cgccaccgtg catgtcttcc
    14041 accggcgtcc gcgcccgttc atgcacgaca cgcactttct cctcggcctc atcggcatct
    14101 gcgtcctgat ggcgctgctc ctgtatccgc tcacccgcta catcacccgt ccgctcaagc
    14161 gcctcacgca ctcggcgcac cgcattgcgg gcggcgactt cgaccaggtc gtcgcggtgc
    14221 cgtcgaatga cgagatcggc gagctcgcgg cggcgttcaa tacgatggcg gaacggatca
    14281 gccacatgat ccgggggacc cgggagctca cggcgaatgt gtctcacgaa ttacgctcgc
    14341 cgctggcgcg gatgcgtgtc gcgctggagc tgctcgatgc gaaggtccgc gacgctcgcg
    14401 ccgccggcgc cggtgagctc atcgcgtcga tcgaggcgga gatcgtcgac atggaccggc
    14461 tcatcgaccg gatcctggag cttgcgaaac tcgacctccg ggaggcggac acgccaatcg
    14521 atgcgataga cctcgacgcg ggcctgaacg ccatcatcgc gaaattcatg cccacgattg
    14581 agcgccggcg gctcgccctc acgctgaccg gcgccggggg ggcgatcgtg cgcggagtcc
    14641 cggacgatat cgcgaccgcg ctcgggaacc tcgtcgacaa tgccgtgaaa tactcgccgg
    14701 agggaggggc cgtcgatatc gccgtcacgc aatccgacgg cgtcgtttcg atcgccgtcg
    14761 ccaatgccag cccgcccctc gccgacgacg aactcgcggg catcttcgag ccattcgttc
    14821 gcctccgccc gacgggcgcg cccggccacg gtctcgggtt ggccatcgcg aaacggatcg
    14881 tcgagcggca tggcggcagc attcgcgccg agcgctccgt gaatggaata cgaattacga
    14941 tcacactccc cgcgtagtca gataacagaa atccccggct gcaccgcaac cggggattca
    15001 ttctgtggga tgaaacgttt cccgtttagt cgattagcac ttattgtagc atgccgtata
    15061 ggagacctcg cacgcggcct tttccgcggc ggtcttctcg accttcttcg tgaccatctt
    15121 tcccttttcc cgtaccttct tctcctcggg ggctatgcac ttgtcgcgag cggccgagca
    15181 ggcgccacgg catgcgagga ctttgggact tgtcgcacag tagaatgcgc cgccgaagac
    15241 ggtgaggaca aaaagaacga ccaacatctt tttcatggta cagctcctta catgatagaa
    15301 tttgcgaacc ctgacatacg cccacggcat acgcaagcat tatttttcgt ccgatcgcgc
    15361 ccctcacggc gccgccgcgc cgaagaccct cgcatgctca atcttgatgc atttcgactg
    15421 caccaccgca atcccggcgg cgcgcgccct ctccgccgat tcgcggtgcg cgagccccag
    15481 ctgcatccac acggcgcccg cgcggaccgc gatcgcctca tcgacgatgc cgggaatcgc
    15541 ctcgaccgcg cggaagatgt cgacgatgtc gatcgcctcc ggaatcgacc ggaggtcggg
    15601 atagcatttc tcgcccagga tctccgcctt caccggattg acggggatga tccggtaccc
    15661 gcgcgcctgc agataggccg cgacgcgatg gctgtcgcgg tcgggcttgt cagacaggcc
    15721 cacgatggcg atggtcttcg cgcgccgcag cagtgccgtg atctcctctc ccggcggatt
    15781 ggcgtcgggc atctcgcatc gggtcgtgtc gttcatgatt atccccctga tacagtactg
    15841 gtacaaccgg cgccagtgcg ccgcgatcag gccgcctcgc cgatgccgta ctcccgtccg
    15901 atgctctcca ggagttgctt ctcctccggc gagacgttcg cgtcggcctc ggcggccttc
    15961 ttcatgagcg catagatctt ccggcgcttc ttcatgtcct cgggcatgcg gatcacgagg
    16021 ctcccggagc gcgccatggc gaagaagggc tccaggcgtg tgatgtcgta tccccatttc
    16081 ttcgccagct cctcggcgaa gcggcgctcc tcgtcggcat agacgccatc ggcggcgatc
    16141 atcaccatca cattgttgaa ggcgaagtcg cgcacgtcat ggagcgtatc gacgagccgc
    16201 gcgcccaccg agtacgatac cgcgtcccgg ttcgtccgca ggtactcctc gtactcggcc
    16261 acgctcatga catctgtgat gatccactcg gagccggtgc tgttcatggg cacgccgcag
    16321 tactcgcact cgatcctgag cgagttggac accggcgcgc
//
LOCUS       DAORHF010000056        15943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2753_length_15943_cov_5.312941, whole genome shotgun sequence.
ACCESSION   DAORHF010000056 DAORHF010000000
VERSION     DAORHF010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2753_length_15943_cov_5.312941"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..955)
                     /gene="amrB"
                     /locus_tag="PKJ16_06835"
     CDS             complement(86..955)
                     /gene="amrB"
                     /locus_tag="PKJ16_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV46739.1"
                     /translation="MGQKNPVGRLIPALALFALACAGPAGHAVQYSVASDDAREFDAA
                     IARPIAYRPPRGRIIGGIVSHHLLAGRHIAGFFSALRASIPASGPAPAFIIIGPNHRS
                     RGGHLVSLSRRPWKTPYGVLAPHPSLATAIANATGAGIDEDAFYNEHAIGALAPFVQH
                     YFPDASMVPVLINYRLAWEDARALGARIAAVARENVIVILSADFTHDKSDRHARENDM
                     RFRELLRRFPAAGPEDIARIELDCRKGLYTMAAFLEARGRSTPHLLGSATSRDIAGPR
                     VPMTSYSFVLFGE"
     gene            complement(925..3162)
                     /locus_tag="PKJ16_06840"
     CDS             complement(925..3162)
                     /locus_tag="PKJ16_06840"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HNV46740.1"
                     /translation="MKRKKIIPAALAIGITACVALSCSGDRTTAPAHLFAAETKGDRF
                     WENPSGEVPGKMYLRCAAVPFKSKQALLATPVHKRYKCTEYAFNGAERSHLNNTGFYL
                     KRVNPTRLAYITYDDMVDLYMHHAGAEKPRAVVELTEEDSAPSYAMAPVFITADYLLH
                     AYHLIFMRMVEDIETNKFYPIIRALTESLLAESLTHYARAKEGRLRDVYRRIAAYFAV
                     PAAIMKIPVSDRSIQGFIAAELAGIQRAAGVSRSAITGEEEDFTQYTPRGHYNKSETL
                     RTYFKVMMWYGRIYFPATRPLEGMIISTLLSKKANREKWEAIYTPTSFLVGESDDLTF
                     LDYASAITTVFGASFNPNAYPDAGKLKEMAKVLKGMRNPRIISIGVRDSAKVPTHKEA
                     QDKTKGFRFMGQRFIPDSYVFTMLTSPRVGSDAHPRNMPKGLDVMAVLGSKDAEAMLA
                     TDKKNVPRYKETFGKLKDEFGALNDAAWRQNVYWRWLDSFRSLIHEHPAPASLPAFAR
                     TPEWRLRLLLAAHGSWAELRHDTILYAKQSYAEMGGPEPVTIYYAGQPAVPRGYVEPN
                     PVFFGKVRALAGETARLIGTAGVLTDEYKQKLAILERFSGACEALARKQEKGAAMGDD
                     DFLWIANASRELQSIVLPNQADHIEQQYKKMALIADVHTDAAGGQVLEVGVGLPREMF
                     VFVKDANGARPCIGYTFSYYEFAHPMNDRMTDEAWRAKAYDPAAKKYLADREPAWVKK
                     IPSVD"
     gene            3385..3945
                     /locus_tag="PKJ16_06845"
     CDS             3385..3945
                     /locus_tag="PKJ16_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46741.1"
                     /translation="MAEDCNTSMNTLLGTFAQQARDYITEFEDILKESETENVERLFV
                     KMDEIKSLEPLPKDSTSKSLQNYKRARELYNEAYRLMAKTIGSFLSRNFSSGTHDRKE
                     SFGYKKSAIPAQAAMRIALLHHVPDKVDIILAALNHDDPDVQRIGVQEYMKLDASAKS
                     NTTNFPQPDVVRARVRQLRKELEYED"
     gene            complement(3952..4608)
                     /locus_tag="PKJ16_06850"
     CDS             complement(3952..4608)
                     /locus_tag="PKJ16_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46742.1"
                     /translation="MTQASMTALQGLRDLTMIKWYVIPLLAITFYIYATEMRQARRTG
                     DWNAVFAGLTVFGMDFINETWNGWVLAIGGRSAFWTAPGDTALRTMVGWNIEIMFMFL
                     IAGIIFHHTLSEDFRHKKILGVPEDWFWAIAFSAFCVFVEVLLNIGGHLVWEYPFWHA
                     SFGGVWLIFLIGYFHFFAAAILVIRMKMKRYKLWTLAAIYAIAILMNVIGMGVMGWKY
                     "
     gene            complement(4630..6213)
                     /locus_tag="PKJ16_06855"
     CDS             complement(4630..6213)
                     /locus_tag="PKJ16_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV46743.1"
                     /translation="MKGIADAGFEECMPVQAATFTHTLNGTDVLVQSQTGTGKTVAFL
                     ISIYQLMLTDDRYKDGKALIIVPTRELAVQIEDEAKLIGAHLPLRIGSFYGGVGYNQQ
                     DRLLEKGVDIMIGTPGRLLDYSNSNKIDFEEVKILVIDEADRLFDMGFYPDLRRMLRQ
                     MVPARKRMTMLYSATLGNDVKRLSSDYMNEPEEITIAPEKVTVELVTQRLYHVGRSEK
                     FRLLLGILKKENPNNALIFTNTKRAAVEIAKRLEYNGYPCEFIMGDLPQSKRLKIIDD
                     IKDGKIRFLVATNVAARGLHIDDLDLVVNYDLPQDEEDYVHRIGRTARAGKSGMAISL
                     ACPEFVYSLEAIEDYTKMKIPVEWPDESLFAEDKSKGVRMAPLRDKRDRDRGRGDRPR
                     GDRKRRRDEKPAARREHARPAERAPHKKRETAEEPGREPRAHHEKRDRHAARAEGDTT
                     QRDAKAKRPRRKRGGKKKSTDERLEQYREKYGEDFTVKNETHRASRKDGTDKKRAQPE
                     KSKKGILDRIKGIFGKKKK"
     gene            complement(6332..7654)
                     /gene="rfaE1"
                     /locus_tag="PKJ16_06860"
     CDS             complement(6332..7654)
                     /gene="rfaE1"
                     /locus_tag="PKJ16_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HNV46744.1"
                     /translation="MINLNFSAARVLVIGDAILDRYYFGRVSRISPEAPVPIVKVARQ
                     TNTLGGACNVVHNIAHLGAASRLVGVAGRDADRTVVEALLGDIGVEHTLFDTGTPTTT
                     KVRVIGEHQQVVRLDFEEVAPIVSALADEIIALVNAELPGVGAIVISDYGKGVCTPEL
                     CQRIIGAARERGRFVVVDPKGRDWSKYAGASIITPNVNELSDVAGTTIPNEDGPIANC
                     GREILERYDLASLLVTRSDRGMSLVTREGVTHIPTEALEVFDVSGAGDTVVATLAVSV
                     ASGLDLAKAVTIANRAAGVVVGKFGTAPIEFEELVHFVSGRIESKVVAHAMLPSIISR
                     LHFKGKRVVMAFGTFSAMDLATITTLKKARAIGDVLIVGIEESAAPDDAAARAELVAS
                     IEHVDFVTIARAERIGEMLGREPVDAIAVSAHSPLLDEAPASIPRMTV"
     gene            complement(7651..8154)
                     /locus_tag="PKJ16_06865"
     CDS             complement(7651..8154)
                     /locus_tag="PKJ16_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HNV46745.1"
                     /translation="MRGEIERFAAAAIEALRAGKKILIMGNGGSAADSQHFAAELVSR
                     FKKERTGLPAIALTTDTSIITAIGNDYHYNRVFARQVEALGSPGDLVLGISTSGNSAN
                     IVEAMHSARAAGCRTVGLLGRDGGAIAPLVDLPVIVRHQNTPRVQEGHLLIIHVVCEI
                     IEETLFQ"
     gene            8386..11388
                     /locus_tag="PKJ16_06870"
     CDS             8386..11388
                     /locus_tag="PKJ16_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013998634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNV46746.1"
                     /translation="MPAHAIPQARYRIEVFYRVHDARARVLAAKLRRLGFRVDAVVIT
                     DNYLVNAAITDEQARAAADMLAQRVTQGHAVNQPYLPERFDQAVEIGFLPGVTDNVAT
                     TAREAIEDLAKLPLDPETSVFTTTTYFFAGALDRDAAERIGAELANPLIQRVKILSAD
                     EYRAAGGMGMAIPVVTLHERPEADVVSLAVSDDELLRLGREGIADPDGTRRGPLALDM
                     LSLGVIKNYFQNIEKRDPTDVELESIAQTWSEHCKHTIFAAKIDEIDEGIFKACIRAA
                     TERVRREKGKDDFCLSVFSDNAGGIIFDDEWIVADKVETHNSPSALDPFGGSITGIVG
                     VNRDALGFGMGARPIANRYGFCFADPFREAPLYRTTDRESRLLSPRRIMEGVIHGVNV
                     GGNCSGIPTPQGFVYFDDRYSGKPLVFVGTVGLIPREVNGAPSVEKRAMPGDRIVMVG
                     GRVGRDGIHGATFSSEALSSGSPATAVQIGDPITQKKLSDAIVAEARGMGLYRSITDN
                     GAGGLSCSVAEMARECGGFEVDLELVPLKYHGLAPWQIWVSESQERMTLAIPPDKLDA
                     FLDLMRRRGVEATAIGAFTARERGIVRYRGKTIFDLDMSFLHDGLPRKTLTTTYTPKK
                     NPYPDFPDPAEPSAVFADMVARLNTASFAFISHQYDHEVQANSCIKPLQGRGRVNANA
                     TVIRPRYDSPRGVVLSQGLYPSYGDIDPYRMAACAIDTAVRNAVAVGGSLDVLALLDN
                     FCWCDSTNPERLGELKLAARACYDYAVAYGTPFVSGKDSMFNDFRGYDEHFNEVMISA
                     PPTLLVSSIGVVEDVGFCQTIDFKFPGDLIYLIGETLDETGGSEYLAYMGERTSGARY
                     IGDTAPSVDAAKNATAYRALSAAMRRGLVASSISVERGGLGVALAKSAMAGMLGCGID
                     LSAVPTKGVSRNDTILYSESQGRVLVSVDPNNADAFETAMRGVALGRIGAVTNDDSVC
                     VKGTNGGNIITASVDALLGRYRGVFKDF"
     gene            11674..12504
                     /locus_tag="PKJ16_06875"
     CDS             11674..12504
                     /locus_tag="PKJ16_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HNV46747.1"
                     /translation="MKAQTTSTAQPRTLVLTGYGINCDNETRYAFERAGARADIVHIN
                     DIIETPSMLDGYQIFSFPGGFSYGDDTGSGKALANRIKNNLLDEFLAFVGRDTLVLGI
                     CNGFQVMANLGLVPALGGFRGDAEVSLEHNESSRYECRWVDLAVERACPSVFIRGIER
                     LHIPIAHGEGNFYAPEEILAAIETRYLACMRYVLPDGSPANGVFPHNPNGALHDIAAV
                     CDPSGRLMGMMPHPERNILPTHRDDWTLRREHARRDGVTLPEEGEGMAIFRNAVGYFR
                     "
     gene            12640..13908
                     /locus_tag="PKJ16_06880"
     CDS             12640..13908
                     /locus_tag="PKJ16_06880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV46748.1"
                     /translation="MNTVKCLLAACAVLLITSAAHAAGSVYVLCYHTFINGKKSMYDF
                     SIDELRHQVNYFKSKGFTFVSFTDIEKGKVRGDKNILVSIDDGNESVYRAYKEVLKPN
                     GIKPLLGIYPCVIERKDYAMKWHQIRELAYEGCDIASHGYYHMKMNKKFYENKRKDFL
                     FEINKSKEVIEQKVNRPVTVFIYPFGLYIKEVYDHLQRAGYKYAMNIRGSALAVPLRA
                     GASFEMPRYLLTRGNINATMAAIHKMADRRNRIAAAGLDIGDDNKIVYSVDGARLTKA
                     GRHAPATRAADVRDARAATDRTAPMVQSPHAKRAVEKHAADRVEPRAGDASRRAAPIL
                     RRARPADNRYVAEAPAPKQKAEGRTVVRERRKESFGLIDRPERVRSGSEKRPGFRALS
                     EGEYNVTSRAMKIDLAGEEARDAKRDDRTR"
     gene            complement(13960..14583)
                     /locus_tag="PKJ16_06885"
     CDS             complement(13960..14583)
                     /locus_tag="PKJ16_06885"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNV46749.1"
                     /translation="MRLALMGPPGSGKGTQAARLCADLGIAHISSGDILRGEVARKSD
                     FGLRIKEFMDRGEIGPQELITDVVLAHIGRTCPDGFLADGFPRTRYQAERLDERFPVR
                     PAVLLEAPDEVIVARITGRRTCPGCSAIYHIQTRPPKRDGTCDTCGAALVQRTDDNEA
                     TVARRLEVYRTDTAPVIDFYRERGILRTTDGTAHPDRVYEAIRSLLT"
     gene            14880..15650
                     /locus_tag="PKJ16_06890"
     CDS             14880..15650
                     /locus_tag="PKJ16_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46750.1"
                     /translation="MKKRTSIRRLAILLFLAMIMGASFTCYKDEMDFQTNESNTNSAK
                     RFIQITSNPVSGFTKGGNDGHLYLGNQNLAATTPINRSLAQSFTIPSGTDDTDMYVTG
                     VWVMLRRYDDVTWPFLYDDVTKKGDVVTALIYTNSGGVPGSLLTSSPGAFIARVSSGS
                     VTYPGDMVAFTFSSRPRLAKGQTYWVVFEITWQGDTANPKPVNNRYVNYNTYLDFHVT
                     DGNDTQGEMAWRNYNAGAWDKTGMTNDIIMWVDGITAN"
BASE COUNT         2888 a         4890 c         5117 g         3048 t
ORIGIN      
        1 ccccgccggc atagcgcgcg ggcttctgga cgccggtcag gagcctgttg aattcggtat
       61 cggtcagtcg gggcatgtcg ttacatcact ctccgaacag gacgaagctg tagctggtca
      121 tcgggacgcg cggtcccgcg atgtcgcgcg aggtggcgga ccccagcagg tgcggtgtcg
      181 accgtccccg cgcctcaaga aacgcggcca tcgtatagag gcccttgcgg cagtccagct
      241 caattcgcgc gatgtcctcc gggcctgccg cggggaagcg gcgaagcagc tccctgaagc
      301 gcatgtcgtt ctctcgcgca tgccgatcgc tcttgtcgtg ggtgaaatcg gcgctcagga
      361 tgacgataac gttttcgcgt gcgaccgcgg cgatgcgcgc acccagcgcc cgggcgtcct
      421 cccaggcaag gcggtagttg atgagcaccg gcaccatcga cgcgtcaggg aaatagtgct
      481 ggacgaacgg cgcgagcgcg ccgatggcgt gctcgttgta aaaggcgtcc tcgtcgattc
      541 ccgcgccagt cgcgttcgcg atcgccgtcg cgagcgatgg gtgtggcgca agaacgccgt
      601 acggcgtttt ccacggccgc cggctcagcg agacgagatg gccgccccgc gaacgatgat
      661 tgggcccgat gatgatgaac gccggggccg ggccggaggc cgggatcgac gcgcgcaggg
      721 ccgagaaaaa tccggcgata tgccttcccg ccaggaggtg atggctcacg atcccgccga
      781 tgatccgacc ccggggcggc cgataggcga tcggacgcgc gatggcggcg tcgaattcgc
      841 gggcatcgtc gctggcgacc gaatactgga cggcatgtcc cgccgggccg gcgcaggcga
      901 gcgcgaagag cgccagcgcc gggatcagtc gaccgacggg atttttttga cccatgccgg
      961 ctcccgatcg gcgaggtatt ttttcgcggc gggatcatac gccttcgcgc gccaggcctc
     1021 gtccgtcatc cggtcgttca tggggtgggc gaactcgtaa tacgaaaagg tgtatccgat
     1081 gcagggacgc gcgccgttgg cgtccttcac gaagacgaac atctcccggg gaaggccaac
     1141 gcccacctcg agcacctggc cgcccgctgc gtcggtgtga acgtcggcga tgagggccat
     1201 cttcttgtac tgctgttcga tgtggtcggc ctggttcgga agcacgatgg attgcagctc
     1261 ccgcgatgcg ttggcgatcc agaggaagtc gtcatcgccc atggccgcgc ccttttcctg
     1321 tttgcgcgcg agcgcctcgc aggcgccgga aaaccgctcg aggatggcga gcttttgctt
     1381 gtattcgtcg gtgagcactc cggccgtccc gatgagacgc gcggtctccc cggcgagtgc
     1441 gcgcaccttg ccgaagaaga cggggttcgg ctcgacatag ccgcgcggaa cggccggctg
     1501 accggcatag tatatcgtca ccggctcggg gccgcccatc tcggcgtagg attgcttggc
     1561 gtagagtatc gtgtcgtgac gcagctcggc ccaggagcca tgcgccgcga gcaggagccg
     1621 aaggcgccat tccggcgtgc gcgcgaatgc cgggagcgag gccggcgccg ggtgctcatg
     1681 gatgagcgat cggaacgaat ccagccagcg ccagtacacg ttctgacgcc aggccgcatc
     1741 attgagcgcg ccgaattcgt ccttgagctt tccaaaggtt tccttgtagc ggggcacgtt
     1801 ctttttgtcc gtcgcgagca tcgcttcggc atccttcgat ccgaggacgg ccatcacgtc
     1861 gaggcctttc ggcatgtttc tcgggtgcgc gtcgctcccc acgcgcggcg aggtgagcat
     1921 ggtgaataca taggagtcgg ggatgaagcg ctgtcccatg aagcggaacc ccttcgtctt
     1981 atcctgcgcc tccttgtggg tcggaacctt cgccgaatcg cgaacgccaa ttgagatgat
     2041 gcgcggattg cgcatcccct tgagcacttt cgccatttcc ttgagcttcc cggcgtcggg
     2101 gtatgcgttc ggattgaacg aggcgccgaa gaccgtggtg atggcggacg cgtagtcgag
     2161 aaaggtgaga tcgtcgctct cgcccacgag aaacgaggtg ggcgtataga tcgcctccca
     2221 tttctcgcgg ttcgcttttt tcgaaagcag ggtcgatatg atcatcccct cgagcgggcg
     2281 cgtggccgga aaatagatgc ggccgtacca catcatcacc ttgaaatagg tgcgaagggt
     2341 ctcgctttta ttataatgtc cccgaggcgt gtattgcgtg aagtcttcct cctcgccggt
     2401 gatggcggac cgcgaaacgc ccgcggcccg ctggatgccc gccagctcgg cggcgatgaa
     2461 gccctggatg ctgcggtccg aaacgggtat cttcatgatg gcggcgggga ctgcgaaata
     2521 cgcggcgatg cgccgataca cgtcgcggag gcgcccctcc ttcgcgcgcg cgtagtgcgt
     2581 gagggattcg gcaagcagcg attcggtgag cgcgcggatg atgggataaa acttgttcgt
     2641 ctcgatgtcc tccaccatgc gcatgaaaat caggtggtat gcgtgcagga gatagtcggc
     2701 cgtgatgaac accggcgcca tcgcgtagct cggggccgaa tcctcctcgg tgagctccac
     2761 caccgcgcgc ggcttctcgg cgccggcgtg gtgcatgtag aggtccacca tgtcgtcgta
     2821 cgtgatgtag gcgagccgcg tggggttcac ccgcttcagg taaaagccgg tattgtttag
     2881 atgggagcgc tccgcgccgt tgaacgcgta ctcggtgcat ttgtagcgct tgtggacggg
     2941 cgtcgccaaa agggcctgct tgcttttgaa cggcaccgcg gcgcagcgca ggtacatctt
     3001 cccggggacc tcgccggatg gattctccca gaaacggtcg cccttcgtct cggcggcgaa
     3061 caagtgtgcg ggggccgtcg tgcgatcacc cgaacaggat agggcgacgc atgctgttat
     3121 gcctattgcc aatgcggccg ggatgatttt tttccttttc atggcgccac ctcgcgaaat
     3181 ctgttccggg atgatagggt gtgtgtcgca tgatggcgca ccgaatgcaa tcattttatt
     3241 acgattccgc ggcgccagtg aagaacggca gcggcaatct ttttcccgcc gcggcgaaaa
     3301 ttgccttgca tgcacaaaaa tccatgcata tgatgatcat cgttggcaga attcaatcat
     3361 cagcatcatg gatcggggga caacatggcc gaagattgca acacttcgat gaacaccctg
     3421 ctggggacct tcgcgcagca ggcgcgcgat tacatcacgg agttcgagga tatcctgaag
     3481 gagtccgaaa cggaaaacgt ggagcggttg ttcgtcaaga tggacgagat taagtcgctt
     3541 gagccgcttc cgaaggacag caccagcaaa tcgctgcaga actacaagcg cgcccgggag
     3601 ctttacaacg aagcgtaccg tctcatggcg aagacgatcg gttcattcct ctccaggaac
     3661 ttctccagcg gcacgcacga caggaaggag tctttcggat ataaaaaatc ggcgattccg
     3721 gcccaggcgg cgatgcgcat tgcgttgctg caccatgttc cggacaaggt cgacatcatt
     3781 ctggccgccc tgaaccacga cgatcccgac gtgcagcgta tcggcgtcca ggaatacatg
     3841 aagctcgacg ccagcgcgaa gagcaatacc acgaatttcc cgcaaccgga cgtcgtcagg
     3901 gcgcgtgtgc gccagctgcg gaaggaattg gagtacgagg attagtggcg gtcagtactt
     3961 ccagcccatg acgcccatcc cgatgacgtt catcaggatc gcgatcgcgt agatggcggc
     4021 cagcgtccag agcttgtatc gcttcatttt catgcggatc acgaggatcg ccgcggcgaa
     4081 gaagtggaag tagccgatga gaaatatcag ccataccccg ccgaatgacg cgtgccagaa
     4141 ggggtactcc cacaccaggt ggccgccgat gttgagcagc acctcgacga agacgcagaa
     4201 ggccgaaaag gcgatcgccc agaaccagtc ttccgggacg cccaggattt tcttgtgccg
     4261 aaagtcctcc gagagggtgt ggtggaagat gatgccggcg atgaggaaca tgaacatgat
     4321 ctcgatgttc cagcccacca tggtgcgcag cgccgtgtcg ccgggtgcgg tccagaaggc
     4381 cgaacggccg ccgatggcca gcacccagcc gttccatgtc tcgttgatga agtccatccc
     4441 gaagacggtg agtccggcga agacggcgtt ccagtcgccg gtccgccggg cctgcctcat
     4501 ctccgtggcg tagatataga aggtgatcgc gagaagcggg atcacgtacc acttgatcat
     4561 ggtgagatcg cgcaatccct ggagcgcggt catcgatgcc tgcgtcatgg gagacctccg
     4621 tggacggtgc tatttcttct ttttgccgaa aattcccttg atccgatcga ggatgccctt
     4681 tttcgacttt tccggttgcg ctcgcttctt gtccgttccg tctttgcggg aggcgcgatg
     4741 cgtttcgttc ttcaccgtga agtcttcgcc gtatttctcg cggtattgct ccaggcgctc
     4801 gtccgtcgat ttctttttac cgccccgctt gcgccgcggg cgcttggcct tcgcgtcgcg
     4861 ctgcgtcgtg tcgccctcgg cgcgcgcggc gtgccggtcg cgtttctcgt ggtgcgcgcg
     4921 cggttcgcgt ccgggctctt ccgcggtctc gcgtttttta tgcggcgcgc gttcggccgg
     4981 acgggcgtgc tcgcgacgcg cggcgggctt ctcgtcccga cgccgcttgc ggtccccccg
     5041 cggccggtcg ccgcgtccgc ggtcccggtc gcgcttgtcg cggagcggcg ccatgcggac
     5101 gcccttgctc ttgtcctcgg cgaagaggct ctcatccggc cactcgaccg ggattttcat
     5161 cttggtgtag tcctcgatgg cctcgaggct gtagacgaac tcggggcagg cgagcgagat
     5221 ggccatgccg ctcttcccgg cgcgcgccgt gcggccgatg cggtgcacgt agtcctcctc
     5281 gtcctgcggg aggtcgtagt tcaccaccag gtccaaatcg tcgatatgga ggccccgcgc
     5341 ggcgacgttg gtcgcaacca gaaagcggat cttcccgtcc ttgatgtcgt cgatgatctt
     5401 gagccgcttg ctctgcggca ggtcccccat gatgaactcg cagggatagc cattgtactc
     5461 caggcgcttg gcgatctcca ccgccgcacg cttggtgttg gtgaagatga gggcgttgtt
     5521 cgggttctcc ttcttgagta tgcccaggag gaggcggaac ttctcggagc ggcccacgtg
     5581 gtacagccgc tgcgtcacga gctccacggt caccttctcg ggcgcgatgg tgatctcctc
     5641 gggctcgttc atgtaatcgc tcgagaggcg cttcacgtcg ttgcccagcg tggcgctgta
     5701 gagcatggtc atgcgcttgc gcgcggggac catctgccgc agcatccgcc ggaggtccgg
     5761 atagaagccc atgtcgaaga ggcggtcggc ctcgtcgatg acgaggatct tcacctcctc
     5821 gaaatcgatc ttgttcgagt tactgtagtc caacaggcgg cccggcgtgc cgatcatgat
     5881 gtccacgccc ttctcgagca ggcggtcctg ttgattgtat cccacgccgc cgtagaagct
     5941 cccgatgcga agggggaggt gcgccccgat gagtttcgcc tcgtcctcga tctggacggc
     6001 gagctcgcgc gtggggacga tgatgagcgc cttgccgtcc ttgtagcggt cgtcggtgag
     6061 catgagctgg tagatggaga tgaggaaggc gacggtcttg ccggtgcccg tttgcgactg
     6121 gaccagcacg tccgtgccgt tcagggtgtg cgtgaaggtc gccgcctgca cgggcatgca
     6181 ctcttcgaag ccggcgtcgg ctattccctt catgatgttt tcgtggagat tgagctcgct
     6241 gaatttcata tgcggtcgtt ttcttttctc tcgtggtttt atgacgccat tcagcgcgat
     6301 gtcgatattt tgtcaataca tatcggaccg cttagacggt catgcgcggg atggaggccg
     6361 gggcctcgtc gaggaggggc gagtgcgccg ataccgcgat ggcgtcgacg ggctcgcggc
     6421 caagcatctc gccgatccgc tcggcgcgcg cgatggtcac gaagtcaacg tgctcgatgc
     6481 tcgcgacgag ctcggcgcgg gcggccgcat cgtccggcgc cgccgactcc tcgatgccca
     6541 cgatgaggac atcgccgatc gcccgcgcct tcttgagcgt ggtgatcgtc gccaggtcca
     6601 tcgccgagaa ggtgccgaag gccatcacca cgcgcttccc cttgaagtgc agccggctga
     6661 tgatggacgg cagcatcgcg tgcgcgacca ccttggactc gatccgtccg ctcacgaaat
     6721 ggaccagctc ctcgaactcg atgggcgccg taccgaactt gcccaccacc acgcccgcgg
     6781 cgcgattggc gatcgtgacc gccttcgcga ggtccaggcc ggaggcgacc gacaccgcga
     6841 gcgtcgcgac caccgtgtcg cccgcgccgg agacgtcgaa cacctccagc gcctcggtgg
     6901 ggatgtgcgt gacgccctcg cgggtgacca ggctcatgcc gcggtcggag cgcgtgacca
     6961 gaagcgaggc gagatcgtag cgctccagga tttcgcggcc gcagttcgca atcgggccgt
     7021 cctcgttcgg gatggtcgtt ccggccacat cggacagctc gttcacgttg ggggtgatga
     7081 tggacgcccc cgcgtacttg ctccagtcgc gccccttcgg gtccacgacc acgaagcgcc
     7141 cccgctcccg cgccgcgccg atgatgcgct ggcagagctc cggcgtgcac acgcccttgc
     7201 cgtagtcgga gatgacgatg gcgccgacgc ccggaagctc cgcgttcacc agcgcgatga
     7261 tctcgtcggc cagcgccgag acgatgggcg ccacctcctc gaagtcgagc cgcaccacct
     7321 gctggtgctc gccgatcacg cgcaccttcg tcgtcgtcgg cgtgcccgtg tcgaagagcg
     7381 tgtgctccac gccgatgtcc ccgaggagcg cttcgacgac ggtccggtcg gcgtcacgcc
     7441 cggcgacgcc gaccaggcgc gacgcggcgc cgagatgcgc gatattgtgc accacgttgc
     7501 aggcgccgcc cagcgtgttc gtctggcggg ccaccttcac gatgggcacc ggcgcctcgg
     7561 gcgagatgcg gctcacgcgc ccgaagtagt agcggtcgag gatcgcgtcg ccgatcacca
     7621 ggacgcgcgc ggcggagaag ttcaggttga tcattgaaag agcgtttcct ctattatttc
     7681 gcagaccacg tggatgatga gcaggtgccc ctcctgcacc cgcggcgtgt tctggtgccg
     7741 cacgatgacg ggaaggtcca ccagcggggc gatggcgccc ccgtcgcgtc ccaggaggcc
     7801 cacggtgcgg cagcccgctg cgcgcgcgct gtgcatcgct tcgacgatgt tggcgctgtt
     7861 gcccgaggtg gagatcccga gcacgaggtc gccgggcgat ccgagcgcct ccacctgccg
     7921 cgcgaacacg cggttgtagt ggtaatcgtt cccgatggcc gtgatgatgg aggtgtcagt
     7981 ggtgagcgcg atcgcgggca ggcccgtgcg ctccttcttg aagcggctca cgagctcggc
     8041 ggcgaagtgc tggctgtcgg ccgcgctgcc cccgttgccc atgatgagga tcttcttccc
     8101 cgcgcgcagc gcctcgatcg ccgcggcggc gaaccgttcg atctcgccgc gcatcgcgcc
     8161 gagcgtcgcg aaaagcgcct ggtgctcggc gagcgaatca tcgaatatgt tcatcatgtc
     8221 gggctctccg tcgctgtgga atggcggggg tatcgttcgt tcgagcggga atgtcaagtt
     8281 gaattttccc gttcggaaag tgataaaatc ttcttgccaa gttcgcgcgt ctggcggcga
     8341 ctatcaccgt cgaccgtatc ttgtccagag gaagcaccga ttcccatgcc cgcgcacgcc
     8401 atcccccaag cccgataccg catcgaggtg ttctaccgcg tccacgacgc gcgcgcgcgg
     8461 gtgctcgcgg ccaagctccg gcggctcggg ttccgcgtcg acgcggtcgt catcaccgac
     8521 aactatctcg tgaacgccgc catcaccgac gagcaggcgc gcgcggcagc ggacatgctc
     8581 gcgcagcgcg tcacgcaggg acacgccgtc aatcaaccgt acctcccgga gcggttcgac
     8641 caagccgtcg agatcggctt cctgcccggc gtgaccgaca acgtggcgac caccgcgcgc
     8701 gaggcgatcg aggacctcgc gaagttgccg ctcgatcccg agacctcggt cttcaccacg
     8761 accacgtatt tctttgccgg cgcgctcgat cgcgacgccg cggagcggat cggcgccgag
     8821 ctcgcgaacc cgctcatcca gcgcgtgaag atcctctccg cggacgagta ccgcgccgcg
     8881 ggcggcatgg gcatggccat cccggtggtc acgcttcacg agcggcccga ggccgacgtg
     8941 gtgagcctgg cggtctccga cgacgagctc ctgcggctgg gcagggaggg catcgccgat
     9001 cctgacggca cgcggcgcgg accgctcgcg ctcgacatgc tgtcgctggg cgtcatcaag
     9061 aactatttcc agaacatcga gaagcgagac cccaccgacg tggagctcga atcgatcgcg
     9121 caaacctgga gcgagcactg caagcatacc atcttcgccg cgaagatcga cgagatcgac
     9181 gaggggatat tcaaggcctg catccgcgcg gccaccgagc gcgtccgccg agagaagggg
     9241 aaggacgatt tttgcctctc ggtcttctcg gacaatgccg gcgggatcat cttcgacgac
     9301 gagtggatcg tggccgacaa ggtcgagacg cacaacagcc cgagcgccct ggaccccttc
     9361 ggcggctcca tcaccggcat cgtgggcgtg aaccgcgacg cgctgggctt cgggatgggc
     9421 gcgcgcccca tcgccaaccg ctacggcttc tgtttcgccg atccgttccg ggaggcgccc
     9481 ctctatcgaa ccacggaccg cgaatcgcgc ctgctctcgc cgcggcgcat catggaaggg
     9541 gtgatccacg gcgtgaacgt tggcggcaac tgctcgggca tccccacgcc gcagggattc
     9601 gtgtatttcg acgaccgcta cagcggcaag ccgctcgtct tcgtggggac ggtcgggctc
     9661 atcccgcgcg aggtcaacgg cgcgccgtcg gtggaaaagc gcgcgatgcc cggcgaccgc
     9721 atcgtgatgg tcggcgggcg cgtgggccgc gacggcatcc acggcgcgac cttctcctcg
     9781 gaggcgctct cgtcgggcag ccccgcgacc gcggtccaga tcggcgaccc catcacccag
     9841 aaaaaactct ccgacgccat cgtcgccgag gcgcgcggga tggggctgta ccgctccatc
     9901 accgacaacg gcgcgggggg gctctcctgc tcggtggcgg agatggcgcg cgagtgcggc
     9961 ggcttcgagg tggacctcga gctcgtgccg ctcaagtacc acggcctcgc cccctggcag
    10021 atctgggtga gcgagtcgca ggagcgcatg accctggcga taccgccgga caagctcgac
    10081 gcgtttctcg atctcatgcg ccggcgcggc gtggaggcga cggcgatcgg cgccttcacc
    10141 gcgcgcgagc gcggcatcgt ccgctaccgg ggaaagacaa ttttcgatct cgacatgtcc
    10201 tttttgcacg acgggctgcc gcgaaaaacg ctcacgacga cgtacacccc aaagaagaac
    10261 ccgtatcccg actttcccga tccggccgag ccgtccgccg tgtttgcgga catggtcgcg
    10321 cgcctcaaca ccgcgagctt cgcgttcatc tcgcaccagt acgaccacga ggtgcaggcg
    10381 aattcgtgca tcaagcccct gcagggcagg gggcgggtaa acgcgaacgc cacggtcata
    10441 cggccgcggt atgattcgcc gcgcggcgtg gtcctctcgc aggggctcta tccgtcatac
    10501 ggcgacatcg acccgtatcg aatggccgcc tgcgccatcg acaccgccgt acgaaacgcg
    10561 gttgcggtcg gcggctcgct cgatgtcctg gcgctgctcg acaatttctg ctggtgcgat
    10621 tcgaccaatc ccgagcggct cggcgagctc aagctcgcgg cgcgcgcctg ctacgattac
    10681 gcggtggcct acgggacgcc gttcgtctcc ggcaaggaca gcatgttcaa cgatttccgc
    10741 ggctacgacg agcacttcaa cgaggtgatg atatcggcgc cgcccacgct gctcgtctcc
    10801 tccatcggcg tggtggagga cgtcggtttt tgccagacga tcgacttcaa gtttccgggc
    10861 gacctcatct atctcatcgg cgaaacgctc gacgaaaccg gggggagcga gtacctcgcg
    10921 tacatgggcg aacggacctc gggcgcgcga tacatcggcg acaccgcccc gtcggtggac
    10981 gccgcgaaga acgcgacggc ctatcgcgcg ctctcggcgg cgatgcggcg cggcctcgtc
    11041 gcgtccagca tcagcgtgga gcgcggggga ttgggcgtgg cgctcgcgaa atcagccatg
    11101 gccggcatgc tcggttgcgg tatcgactta tccgcggtcc ccacgaaggg cgtttcccga
    11161 aacgatacga tactctattc tgaatcgcag gggcgcgtcc tcgtgagcgt cgatccgaac
    11221 aacgcggacg ccttcgagac ggcgatgcgc ggcgtggcgc tcgggcgcat cggcgcagtg
    11281 acaaacgacg acagtgtctg cgtaaaaggg acgaatggcg gcaacatcat aacggcgagc
    11341 gtcgatgcgt tgctgggacg atatcgcggg gtttttaagg atttttaggg cacctcgccc
    11401 ccgtcccctc tcccgcagga gaggggggaa agaagcttct cgcgaggctg agctgaaatg
    11461 aattgatgcg ctcacgacga gcggtccagg gtgagaagga atatattgat atgaggcgat
    11521 ttttccactc accccccggc cccctctctc taaatagaga gggggtggtc aggcgcgagt
    11581 ctagtagtga gaagtccctc tctatgagta gagagggacg cggcagagcc gagggtgagt
    11641 agcacagact cggattagag caataaaacg aacatgaaag cacaaaccac atcaacggcg
    11701 caaccccgga cgctggtcct caccggctac ggcatcaatt gcgacaacga aacgaggtac
    11761 gccttcgagc gggccggcgc gcgcgcggac attgtgcaca tcaacgatat catcgaaacg
    11821 ccgtcgatgc tggacggcta ccagatcttt tccttccccg ggggcttctc ctacggcgac
    11881 gacaccggca gcggcaaggc gctcgccaac cgcatcaaga acaatctgct cgacgagttt
    11941 ctcgcattcg tgggccgcga cacgctggtg ctggggatct gcaacggctt tcaggtgatg
    12001 gcgaacctcg gattggtccc cgcgctgggc ggttttcgcg gggacgcgga ggtgtcgctc
    12061 gagcacaatg aatcgtcgcg ttacgagtgc cggtgggtgg acctcgcggt ggagcgggcg
    12121 tgcccgtcgg tgttcatccg gggaatcgag cggctgcaca tccctatcgc ccatggcgag
    12181 gggaacttct atgcgcccga ggagatcctc gcggccatcg agacgcggta tctggcgtgc
    12241 atgcgctacg tgctccccga cggctccccg gcgaacggcg ttttcccgca caatccgaac
    12301 ggcgcgctcc atgatatcgc ggcggtctgc gacccgtccg gccgcctcat ggggatgatg
    12361 ccccatccgg agcgaaacat cctgcccacg caccgcgacg actggacgct gcgccgcgaa
    12421 cacgcgcgac gcgacggcgt cacgctcccg gaggaggggg aggggatggc gattttccgg
    12481 aacgcggtgg ggtacttccg ctagcgagaa tggcggccgc ccgaaaatga ttcttcccgt
    12541 gggccggatt ggccgaaaat aaccacggtg cgtgtttttt gtttgatgtg ttttggggac
    12601 cgtgcgacgc tatcataatg caacaatgag gatggaaacg tgaataccgt aaaatgccta
    12661 ctcgcggcct gcgccgtgct cctgattaca tcggccgccc acgccgccgg ctcggtgtac
    12721 gtgttgtgct atcacacatt catcaacggg aaaaagtcga tgtacgattt cagcatcgac
    12781 gagctccgtc accaggtgaa ctacttcaag agcaaggggt tcacgttcgt gtcctttacg
    12841 gacatcgaga agggtaaggt tcggggcgac aagaacatcc tggtctccat cgacgacggc
    12901 aatgagagcg tgtaccgcgc gtacaaggag gtgctcaagc ccaacgggat caagccgctg
    12961 ttgggcatct atccctgcgt gatcgagcgc aaggactatg cgatgaagtg gcaccagatc
    13021 cgcgagctcg cctacgaggg ctgcgatatc gcctcgcatg ggtattatca catgaagatg
    13081 aacaagaagt tctacgagaa caagcgcaag gacttcctct tcgaaatcaa caaatccaag
    13141 gaggtcatcg agcagaaggt gaaccggccg gtgacggtct tcatctatcc gttcggcctg
    13201 tatatcaaag aggtgtacga ccacctgcag cgcgccggtt acaagtacgc catgaatatc
    13261 aggggctcgg cgcttgcggt gccgctgcgg gcgggggcga gcttcgagat gccgcgatat
    13321 ctgctcacgc gggggaatat caacgccacc atggcggcga ttcataaaat ggccgaccgg
    13381 cgcaaccgca tcgcggccgc cggtctcgac atcggtgacg acaacaagat cgtctatagc
    13441 gtcgatggcg cgcgcctgac gaaggccgga cggcatgcgc cggccacgcg tgccgccgat
    13501 gtgcgcgacg cgcgcgcggc cacggaccgg actgcgccga tggtccaatc gccgcacgcg
    13561 aagcgcgcgg tcgagaagca cgccgccgat cgcgtcgagc cacgtgcggg cgatgcgtcg
    13621 cgccgcgccg cgccgatact gcgccgcgcg aggcctgccg acaaccgcta tgtcgccgag
    13681 gccccggcgc cgaagcagaa ggcggagggg cgcaccgtcg ttcgtgagcg gcgaaaagag
    13741 tcgtttgggt taatcgaccg gccggagcga gttcgaagcg gctccgagaa gcgccccgga
    13801 ttccgcgcgc tgtccgaggg cgaatataat gtgaccagcc gcgcgatgaa gatcgacctg
    13861 gcgggcgaag aggcgcgcga cgcgaagcgg gacgatcgca cccgctgaca ccttccgaaa
    13921 ccggcgccgt tgcgcgatca acgcggcgca gtcttcgctt tacgtaagca acgaacgaat
    13981 cgcctcgtat acccggtctg ggtgggccgt cccgtccgtc gtgcgcagaa ttccccgttc
    14041 ccggtagaag tcgatgacgg gcgccgtgtc cgtccgatac acctcgagcc gccgcgcgac
    14101 ggtcgcctcg ttgtcgtccg tgcgctggac cagcgcggcg ccgcatgtat cgcaggtccc
    14161 gtcacgcttc ggcgggcggg tctggatatg gtagatggcg gagcaacccg gacaggtccg
    14221 ccggccggtt attcgcgcga cgatgacctc gtccggcgcc tcgagcagaa ccgccgggcg
    14281 cacggggaag cgctcgtcca gacgttccgc ctgatagcgg gtgcggggga aaccgtcggc
    14341 caggaagccg tccgggcagg tgcgcccgat atgcgcgagc accacgtcgg tgatgagctc
    14401 ctgcgggccg atctcgcccc ggtccatgaa ctccttgatg cgcaggccga agtcgctttt
    14461 gcgcgccacc tccccgcgca gtatgtcgcc ggacgagatg tgagcgatac cgaggtcggc
    14521 gcacaatcgc gccgcctgcg ttccttttcc cgagccgggc gggcccatca atgccagtcg
    14581 catgttttgt caccttgctc catggtttcc ggaattccac tggtggctcc ggtttcgcct
    14641 cttcgaatcg aaaggctttt agatttgata cttgtcaata acaatcacgg tattggctag
    14701 caggcgccat tgtcaatttg ttcgcatatt ttcgaaaatg tctaaaatat tctgactgga
    14761 tggtttaaga aaataacttg acgcattatt gcatcattat tatattttgt ctatatggcc
    14821 gactgcggat gacgtatgtc ttcagtcgat ttcttgaatt gagcgatcag ggaaccgtaa
    14881 tgaaaaaaag aacctctatt cggcgattgg ctatactgct tttcctcgcg atgataatgg
    14941 gcgcgtcatt tacgtgctat aaggacgaga tggattttca gacgaatgag tcgaatacga
    15001 acagtgcgaa gcgattcatt cagattacct ccaacccggt ttctggtttt acaaaggggg
    15061 gtaatgatgg acatctgtat cttggaaatc aaaaccttgc cgccacaacc ccaataaatc
    15121 gcagtctggc ccaatcgttt actatcccat ctggaaccga tgacacggac atgtatgtca
    15181 ccggcgtttg ggttatgctg cgtaggtatg atgacgtaac atggccattt ttatatgatg
    15241 atgtaacgaa gaagggagat gtcgtgacag ccttgatcta tacaaactct ggcggagttc
    15301 cggggagttt gctcaccagt tctccgggag ccttcattgc acgcgtaagt tccggttccg
    15361 ttacttaccc gggtgatatg gtggcattta cattcagctc gaggccgcgt cttgcaaagg
    15421 ggcaaacata ctgggtcgtt tttgagatca cctggcaagg cgataccgcg aatcccaaac
    15481 cggtcaataa caggtatgtg aattataaca cctatcttga tttccatgtg actgatggaa
    15541 atgatactca aggtgaaatg gcttggcgaa attataatgc tggggcatgg gataaaaccg
    15601 gtatgacaaa cgacatcatc atgtgggtcg atggcataac cgccaattag tccttctgat
    15661 agatttgaca ctgagatgcc gccctgtgcg gcatttcgtg tctctgcggg attccggtat
    15721 tcaccgccgg aatcgacgat ccggtaaata gtatttgacc tccgccgccg gtgtgttgtc
    15781 gtcttcgtat ggcgttatct tactgaaggc acactgccca tgccgggatt tcatctttca
    15841 atgagcaacc gtctcgagac gctggcggac cttctcgcgc aggatcttgc gcgcgggacg
    15901 gggggcccgt ttgcgacgga gctcctggtc gtgcagagcc ggg
//
LOCUS       DAORHF010000057        15926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2762_length_15926_cov_4.787096, whole genome shotgun sequence.
ACCESSION   DAORHF010000057 DAORHF010000000
VERSION     DAORHF010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2762_length_15926_cov_4.787096"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PKJ16_06895"
     CDS             <1..850
                     /locus_tag="PKJ16_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV46751.1"
                     /translation="LQISHLVFPFEPTWKTEERAIGMIEKEYEDGMDIAFDAYPYAGG
                     NTSIIAPIPVWFLDNFQENIKNAFMMKKLVTLFRLSKKVLKFDMGAIMLLRAFHKPYE
                     KYAGRLFRDIAADLGMDPIDAYLHFAEITEGKARVLLPEFYDNDGRDGIMARVLAHPL
                     CRFETDTLITRKGVQNPATFGTFPRILGRFVREFRLMSLPDAIHRMTGASARRMGIQK
                     RGEIKKGYYADLVIFDPDTVHENPIPDQPPLGIEHVFINGKQAVVNNKVDMKRLHGRV
                     LRKNDQ"
     gene            888..1235
                     /locus_tag="PKJ16_06900"
     CDS             888..1235
                     /locus_tag="PKJ16_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV46752.1"
                     /translation="MITIRHYDDITPIPVDADGAKNARKRLLVGAADGVPYFSIRMFT
                     IGPGGNTPSHAHDFEHEIYILEGTAKCVSGSTETPVRAGSVIFVPGGEPHCFVNTGSG
                     DLVVLCMVPKEYE"
     gene            complement(1239..2168)
                     /locus_tag="PKJ16_06905"
     CDS             complement(1239..2168)
                     /locus_tag="PKJ16_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HNV46753.1"
                     /translation="MKGFIFAAGFGERLRPITDGMPKPLVPVVNIPAICYALALLRRA
                     GIDEIIINLHYRAHDIVRFFAAHGDFGCRLFFSFEEEILGTGGGLKNCERQIDGDECV
                     ILNSDVIMDLDVAALVDAHRRTGDPATVVLHRTPRASEIGMVGVDGDRVIDFKNFLER
                     GARSDYIYSGAAVVSSRLFPYLVPEFSSIVYTGYVELIRNARLGYVAHESCWLDIGSL
                     RSFREANLHMLEDRDGVRSLMCHELGLPQDAISRDARIRDRAVVRNSVIGDGAVLGEG
                     ALVENSVVLSGGVIAANARIVDSVVFGDRVLTA"
     gene            complement(2165..3166)
                     /locus_tag="PKJ16_06910"
     CDS             complement(2165..3166)
                     /locus_tag="PKJ16_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV46754.1"
                     /translation="MDINTTMRDFVSGALDAFDRIAPLEGDASTREYFRVHSGQRTCI
                     LCRDGAAAGTPVDGYPFAAVHGLLHDNGVPVPAILAMDMENGLILQEDAGDDLIETVL
                     PSLSRRGVIDIYARMVDIAVDIQSIRGDGTRLPFNLAFDIDKLMFEFGFFIEHALKGY
                     FHASMTEAAERVLREAFLRIAGELYRPELFVLNHRDYHSRNVMTKGGALFVIDFQDAR
                     MGLPHYDIVSLVRDSYCVLDDDIVGMMMTRHFDALRDRGLLRMSRDEYDRLFDLMAFQ
                     RNVKAVGTFGYQVGRLGKRRYESSIPTTLGYLPAYVSRRPELAAAYDIIRENIGTRA"
     gene            complement(3385..4554)
                     /gene="ftsZ"
                     /locus_tag="PKJ16_06915"
     CDS             complement(3385..4554)
                     /gene="ftsZ"
                     /locus_tag="PKJ16_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008516016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HNV46755.1"
                     /translation="MFKLEEERPFHTTIKVIGVGGAGTNAVNRMIATGMDGIEFIVAN
                     TDAQQLKDSLAQTKIQIGTKLTKGLGAGADPEVGRGAANEDRDKISKALKGADMVFIT
                     AGMGGGTGTGAAPVVAEIAKEHNALVVGVVTKPFRVEGKRRMTVAQEGVTTLKEKVDT
                     LITIPNDQLLKIIDRKTPIDDAFRLADDILRQGVQGIADIIMVTGLVNVDFADVRTVM
                     RETGDALMGVGIGSGENKAVEATQMAIASPLLEETGIEGAKAVLINIAGGNDLSLHEV
                     NEVNDIITRQVDPEANIIFGTTIDPSLDERVRVTVIATGFDRRRNQLNRPGVNDLDKQ
                     TGTSLQLIEGRKPEGAAARRVPVNDQTFKVDKQRISRDFAREDLDIPAFLRRTLD"
     gene            complement(4652..5887)
                     /gene="ftsA"
                     /locus_tag="PKJ16_06920"
     CDS             complement(4652..5887)
                     /gene="ftsA"
                     /locus_tag="PKJ16_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HNV46756.1"
                     /translation="MEDVIVGLDIGTTKTCAVIGYRNENGDVEVSGVGVAPAKGLKNG
                     VIINIDNTISSIGKAIEDAELMAGSEVGGMYVGITGQHMKGENSRGVVAVSNRNRTIT
                     PVEVKRVIEAAQAVVIPADREIVHVLSKEFSVDDQTGIKDPIGMSGVRLEAEVHIVTG
                     GTTCIQNLVKSVNKAGYPVSDIVFSPLASAEATLSQDERELGVVLVDIGGGTTDIMIY
                     VEGGVAYSSVLAIGGIHVTNDISIGLRTPIESAESIKRKYGCAVIDLVDASETIEVPS
                     VGGRAPRRLFKQELAQIIEPRMMEIVEMVEQELVRSGKKDIVAAGAVFVGGGSMIEGC
                     VEAAERVLNMPVRLGVPRDIVGLKDVVATPQFANAVGLLKYGVKMNQYSQGRASRKKA
                     GGGIGAMGSRIRRWFEEYL"
     gene            complement(5896..6609)
                     /locus_tag="PKJ16_06925"
     CDS             complement(5896..6609)
                     /locus_tag="PKJ16_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46757.1"
                     /translation="MMRTRINMVAVMMLGALMLFAATGRAHAFRNVKKVSAIEFKGLK
                     YLTKYELISAADTVVEQDGIVIDVDSLEAALRKNLYVKDYRIVEKDRRITVTVEEKRP
                     ALVMAIEKEGAYAFIELDGELAALSYNRVHAADRPLVVVPWAFAHSGSLSKQVRDYLR
                     TILEAEKSGLKVCRELTEVYIRSDGKVRIRLRGRRTEFLAEMNRAALERLNTCVGFLD
                     AAGRYPAHMNIFDTFGVMK"
     gene            complement(6609..7979)
                     /gene="murC"
                     /locus_tag="PKJ16_06930"
     CDS             complement(6609..7979)
                     /gene="murC"
                     /locus_tag="PKJ16_06930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HNV46758.1"
                     /translation="MFRKSKTMHFMGIGGIGMSGIAEILITLGFQVSGSDLHESEQTR
                     RLRDLGATVFIGHFPSNIGDYSVVVTSSAIGKNNPELIEARSRGIPVIHRAEMLAELV
                     RLKHGIGIAGTHGKTTTSSLLAFVLSHGGINPTAVVGGKVLNFGSNARAGEGEYIVFE
                     ADESDGSFLKLLPTIAVVTNIDADHLDHYKFFEGLKEAFLTYMNNVPFYGYSVLCIDD
                     NVIAELLPRVERPYLTYGFSENADFRAANVRLADGRTHYDCYYKNELLGAIEIGLIGN
                     HNVINSLAVVVVALDLGLDFAAIRAGLAEFAGVGRRMERVGEARGVRVIDDYGHHPTE
                     IRATLAGLKALGRRLVVVFQPHRYTRTQLLWDEFGQAFADADELFLTEIYPAGEEPIE
                     GVSSALIRDSIRKHDTREVRIIGRFEEIAPTLAAAAREGDIVLTLGAGDIHKAGRQIV
                     EALGAR"
     gene            complement(7984..9090)
                     /gene="murG"
                     /locus_tag="PKJ16_06935"
     CDS             complement(7984..9090)
                     /gene="murG"
                     /locus_tag="PKJ16_06935"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HNV46759.1"
                     /translation="MKGKNILIVGGGTGGHISPGIAIYEEFVKRGVHAFVLVGRRDAR
                     FSSLADVKDADLLLYGAPSLTKNPVMLPIVALKFLHAYFWAKRFIARSQIAAVIGMGG
                     YVSGPALLAAARMGVPVYLCEQNSVPGKVTRYFAKRAAAIFTTFAASDEYLKNGVKIV
                     QAGNPIRQRVFAPISCEDARRAFNLGHCKRVILLIGGSQGALRLNELMLGLKTKFSGE
                     FADTGVIWSTGDLSYNEYREKSGRALEKGSVYMSPFIENVGAAYRASDIAISRAGSGV
                     MMEIAAMGLPSVMIPYPFAAANHQDKNADEFVEAGAAVKIANDDATPEIAGPIILDLL
                     GNPMRLAKMAEKALKVARPDAAKTIVDEIITALR"
     gene            complement(9087..10247)
                     /gene="ftsW"
                     /locus_tag="PKJ16_06940"
     CDS             complement(9087..10247)
                     /gene="ftsW"
                     /locus_tag="PKJ16_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HNV46760.1"
                     /translation="MDLKSIIDTRRRGDPDVFLFVTVLLLVGIGIAMSYSASAIHAAR
                     IYQDSFYFLKRQIVWFVLGLGALLVFQEVDYRRYARYTKILLLVSFVLLILVFVPVIG
                     KSAKGSARWLSLGFLQIQPSEIVKLCLVLYLVKVFSSERTGVANHVAQLLVPAAIVAL
                     AFVLLMLQPDFGTAIDILLIAALILFVSGFPILYIAAVGIVSVPMFYLLIYEVSYRKN
                     RLLSYFDPWADRFGKGYHVVQSFIAFKKGGFLGTGLGYGTQKISRLPEPHTDFIFAVI
                     AEETGILGTLLIIALFCTVFWRGLIISLGAPDEYGRLLAVGLTLYIVVQAFINIGVVS
                     GALPTTGLPLPFISYGGSSLVCNMSAAGILLNISRYRAGAYEGVQLTEEVWQ"
     gene            complement(10238..11620)
                     /gene="murD"
                     /locus_tag="PKJ16_06945"
     CDS             complement(10238..11620)
                     /gene="murD"
                     /locus_tag="PKJ16_06945"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HNV46761.1"
                     /translation="MDLSKIQKILIIGLGPRTGVAASNFLARKGYAVVVSDSKDRAAL
                     TDALAALDRGVRVIAGAQRPELLDEGFDLAVLSPGVPRAIPLVAAAVERGVPVVSEIE
                     LAYRFAKGLIVAITGTDGKTTTTSLVGHILAALGIDTFVGGNIGVPFVSFVDRTRDSS
                     VTVLELSSFQLETIDRFRPDVAAILNIAPDHLDRYATMDEYVDAKMRIAMNQRPDDVF
                     VHFADDALIRSRLGAVVARTCGFAVRDTDAAIRFDGSMVRLAVVDAPVLDPTRMRIIG
                     VHNVQNAMAAILMVRAVLARMGRPADDGAIAEAIYSFKGLPHRMEIVGEFEGRTFIND
                     SKATTVNAVEMALASLTRPAVLILGGRTKGDDYARLNGPIAQRVKRLLLIGESTPEFA
                     RLFAAHDPVPVATLEEALAQAMRHSAPGDAVLYSPATASFDMFRNYEERGDRFRETFQ
                     RLVRGEIAWT"
     gene            complement(11717..12838)
                     /gene="mraY"
                     /locus_tag="PKJ16_06950"
     CDS             complement(11717..12838)
                     /gene="mraY"
                     /locus_tag="PKJ16_06950"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HNV46762.1"
                     /translation="MFYHFILPLQEYLSFLRLFQYISFRSAYAALTALVIVLIFGAVV
                     IAWLRRLRFKEEIRSDGPQSHKAKAGTPTMGGVIILGAVLISIFLWGNFNSEYLILLT
                     VATLVLGSLGFADDYIKSIRKRKGGVPGKLKLVVQLATAGGVVAGMLVFPANPEALTQ
                     IYVPFVNEPLVTLSRVAIGGIALDMTWAWVIFAMIVVVGSSNAVNLTDGLDGLAVGTV
                     LFVAASLAVMCYLSGHVKIASYLKIPYIPDGAELTVFLSALCGASLGFLWFNSNPATV
                     FMGDTGSLALGGVIGIVAIMIKKEIFLFIVGGVFVVEAMSVIIQVLSFKLTGKRVFKM
                     APIHHHFELSGWTEQKVVVRFWILGIILAILGLSSLKIL"
     gene            complement(12846..14234)
                     /gene="murF"
                     /locus_tag="PKJ16_06955"
     CDS             complement(12846..14234)
                     /gene="murF"
                     /locus_tag="PKJ16_06955"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HNV46763.1"
                     /translation="MRADFPTTMGTMAAYAGGSLVGGMPDTRIRTISSDSRDLGPESL
                     FVPLVGERFDGHDFIETLAAERTIAGFVSMRESDRATAERHGVPAVLCDDTLRALGRI
                     AARHRDSASARMVGITGTNGKTTTKELVYAIASTALRTHKNEKNYNNEIGVPFALLGI
                     RPEHECAVMEMGMNHAGEIERLSRIVRPEIALITNVGEGHLEFLGSVENVARAKAEIV
                     RGMRPGGTIILNRETACVGILEDAARDAGLRMLTFALDADADLRPESYSLQPDRTEIV
                     LGGVTFAAPLYGVHNAANLLAAIAVGRCLGIDDAAMARALASMDAIDKRSQIVRGEFV
                     VINDTYNSNPLSSRSAFASAARVFSGRRLIAVVSDMKELGAKAARYHAEAGREIAASG
                     FELLFAWGELAAHTVAGAREAGMNGDRVAHFADKQSLVDALKKRVAKDDVVLVKGSRS
                     MKMEEVVADLLR"
     gene            complement(14231..15739)
                     /locus_tag="PKJ16_06960"
     CDS             complement(14231..15739)
                     /locus_tag="PKJ16_06960"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HNV46764.1"
                     /translation="MKRLRELITGIDEPLQVAGDVPDAPIASVDYDSRRIANGSLFVA
                     VEGFQSDGHRFVTAAVDAGAAAAVVSRARLPEFAPLAERGVAIIGSDNTRRALSALSA
                     AFYDNPSAGMTVIGVTGTNGKTSITYMLEAIMRAAGRVPGVIGTVNYRWCGRETAAPN
                     TTPESRDLQQLIAEMRDDGVDTLIMEVSSHGLALGRVDHVAFTAAIFTNLTRDHLDFH
                     ADFEDYFAAKKRLFTLLGGLPGDRAGIVNVDDDYGRRLARDFAGASYPVLGFGMHDAA
                     AYRVDPASVVNSLEGIAYTLSRPDGGGRIALPLAGRFHVYNSLAAVATAHRLGISFDC
                     IRDALAGIAHIPGRFNTIASRLGFHVVVDYAHTDDALRKLLTSVRELRPRRLITVFGC
                     GGDRDRAKRPLMGRAAAELSDHVIVTSDNPRTEDPARIIEDIRAGIDGRAYEVEPDRA
                     RAIAAGIAMASAGDIVVIAGKGHEDYQIVGRAKTHFDDREMARKFIAEREAA"
BASE COUNT         2921 a         5306 c         4953 g         2746 t
ORIGIN      
        1 gctgcagata tcgcacctgg tgttcccgtt cgaaccgacg tggaaaacgg aggagcgggc
       61 catcgggatg atcgagaagg aatacgagga cggcatggac atcgccttcg acgcctaccc
      121 ctacgccggc ggcaacacct ccatcatcgc gcccatcccg gtgtggttcc tggacaactt
      181 ccaggagaac atcaagaacg ccttcatgat gaaaaagctc gtcacgctgt ttcgcctgtc
      241 gaagaaggtg ctcaagttcg acatgggcgc catcatgctg ctgcgcgcct tccacaagcc
      301 ttacgaaaag tatgcgggcc ggctgttccg cgacatcgcc gccgacctgg gcatggaccc
      361 catcgacgcc tatcttcatt tcgccgagat caccgaggga aaggcgcggg tgctcctgcc
      421 cgagttctac gacaacgacg ggcgcgacgg catcatggcg cgggtgctcg cgcacccgct
      481 ctgccggttc gagaccgaca ccctcatcac ccgcaagggc gttcagaacc cggccacctt
      541 cgggaccttt ccgcgcatcc tgggacgctt cgtgcgcgag tttcggctga tgagccttcc
      601 cgacgcgatc caccgcatga ccggcgcctc ggcgcgccgg atgggcatac agaagcgcgg
      661 cgagatcaag aagggctatt acgcggacct cgtcatcttc gacccggaca cggtgcacga
      721 aaacccgatc cccgaccagc cgcccctcgg gatcgaacac gtcttcatca acggcaagca
      781 ggcggtcgtg aacaacaagg tcgacatgaa acgcctgcac gggcgcgtgc tgaggaagaa
      841 cgaccagtaa agacggggca ctgccccgtc tcaaaggagc catcgacatg ataaccatac
      901 gacattacga cgacatcacc ccgatcccgg tggacgcgga cggggcgaag aacgcgcgca
      961 agcggctgct cgtgggagcg gcggacggcg tgccctactt ttccattcgc atgttcacca
     1021 tcggcccggg cggcaacacg ccgtcgcacg cgcacgattt cgagcatgag atctacattc
     1081 tcgaggggac cgcgaagtgt gtgagcggct cgaccgagac gccggtgcgc gcgggttccg
     1141 tcatcttcgt ccccggggga gagccgcact gcttcgtaaa tacgggcagc ggcgacctcg
     1201 tggtattgtg catggtgccc aaggagtacg agtaggtctt acgccgtaag aacccggtcc
     1261 ccgaacacca cgctatcgac aatccgcgcg ttggccgcga tgacgccgcc ggaaaggacc
     1321 acgctgttct ccacgagcgc gccctcgccc agcaccgccc cgtcgccgat cacggaattg
     1381 cgaacgaccg cgcgatcgcg aattcgcgca tcgcgggaga tggcatcctg cggaagaccc
     1441 agctcatggc acatgagcga gcgaacgccg tctcggtcct ccagcatgtg cagattcgcc
     1501 tcgcggaagg aacgcagcga accgatatcg agccaacacg attcgtgcgc aacatacccg
     1561 agacgcgcat tgcggataag ttcgacatag ccggtataga cgatgctcga aaactccggc
     1621 acgagatacg ggaagagtcg cgatgaaacg accgccgcgc cggaatagat atagtccgaa
     1681 cgcgcacccc tttcgaggaa attcttgaaa tcgatcaccc gatcgccatc cacgcccacc
     1741 atgccgatct ccgatgcgcg tggcgttcgg tgcaatacga ccgtcgcggg atctcccgtc
     1801 cgccggtgcg cgtcgacgag cgcagccacg tcgaggtcca tgatcacgtc gctgttgagg
     1861 atcacgcact catcgccgtc aatctgacgc tcgcaattct tgaggccgcc cccggtcccg
     1921 aggatttcct cctcgaatga aaagaacagc cggcacccga aatcgccgtg ggccgcgaaa
     1981 aagcgcacga tatcgtgcgc ccggtagtgg aggttgatga tgatctcgtc gatgcccgcg
     2041 cgcctcagga gcgcgagcgc atagcagatc gccggaatgt tcaccaccgg taccagcggc
     2101 ttcggcatgc cgtcggtgat ggggcgcagg cgctcgccga accccgccgc gaagatgaat
     2161 cccttcatgc gcgcgtaccg atattctctc gaatgatatc gtaggcggcc gcgagctccg
     2221 gccggcgcga gacatacgcc gggagatagc cgagcgtggt cgggatcgac gactcgtagc
     2281 ggcgcttgcc caggcgtccc acctggtagc cgaaggtgcc gacggccttt acgttgcgct
     2341 ggaaggccat gaggtcgaac aggcggtcgt attcgtcgcg cgacatccgg aggaggcccc
     2401 ggtcccgcag cgcatcgaag tggcgcgtca tcatcatgcc cacgatatcg tcgtccagaa
     2461 cgcaatagga gtcgcgcaca agcgagacga tatcgtagtg cggcaatccc atgcgtgcgt
     2521 cctggaagtc gatcacgaaa agcgcgccgc ccttggtcat gacgtttctg ctgtgataat
     2581 cgcgatggtt gagcacgaag agctccgggc gatagagctc gccggcgatg cgaaggaacg
     2641 cctcgcgcag cacccgctcg gctgcctccg tcatcgacgc gtgaaaatat cccttgagcg
     2701 cgtgctcgat gaaaaagccg aactcgaaca tgagcttgtc gatgtcgaag gcgagattga
     2761 acgggaggcg cgtcccgtcg ccgcgaatgg actggatgtc cacggcaata tcgaccatgc
     2821 gcgcatagat gtcgatcacc ccgcggcggg acaatgaggg gagaacggtt tcgatgagat
     2881 cgtccccggc atcctcctgg aggatgaggc cgttctccat gtccatcgcc agaatcgcgg
     2941 gaacggggac gccgttatca tggagaaggc cgtggactgc ggcgaacgga tacccgtcga
     3001 ctggcgttcc ggcggcagcg ccgtcgcggc agagaataca ggtgcgctgc cccgagtgga
     3061 cgcgaaagta ttcgcgcgtg gaggcgtcgc cctcgagcgg cgctatgcga tcgaaagcgt
     3121 cgagcgcgcc agagacgaaa tcgcgcatcg ttgtattaat gtccattgtc acgatggcgt
     3181 cgggagtgcc atcgtacagt gaaatgagca agtataattt gccgcgcggg ttccgcttcc
     3241 cgctgtcccg cccgatggaa ttgcgatgcc ggaccgcccc ccgggggggc cgggggcggt
     3301 ccgtgcgttt atggtcgtat ctgtccggtg cgattcgatg atttcatact gcgaagtatc
     3361 atcatcacta taaaagaaac cgcgctaatc cagcgtgcgc cgtaagaacg ccggtatgtc
     3421 caggtcctcg cgggcgaagt cgcggctgat gcgctgcttg tccaccttga aggtctgatc
     3481 gttcacggga acgcgacgtg cggcggcgcc ctccggcttg cggccctcga tgagctgcag
     3541 cgaggttccg gtctgcttgt caagatcgtt cacgccgggc cgattgagct ggttgcggcg
     3601 ccggtcgaaa ccggtcgcga tcaccgtgac gcgcacgcgc tcgtcgagcg acggatcgat
     3661 ggtggtgccg aagatgatgt tggcctccgg atccacctgc cgggtgatga tgtcgttgac
     3721 ctcgttcacc tcgtggagcg agaggtcgtt cccgccggcg atgttgatga gcacggcctt
     3781 cgcgccctcg atcccggtct cctcgaggag ggggctcgcg atggccatct gcgtcgcctc
     3841 caccgccttg ttttcgccgc tgccgatgcc cacgcccatg agcgcatcgc ccgtctcccg
     3901 cataacggtg cgcacgtcgg cgaaatcgac gttcaccagg ccggtcacca tgatgatgtc
     3961 ggcgatgccc tgcacgccct ggcggaggat gtcgtcggcc aggcggaagg cgtcgtcgat
     4021 gggggtcttc cggtcaatga ttttcaggag ctggtcattg ggtatggtga tgagcgtgtc
     4081 gaccttctcc ttgagggttg tcacgccctc ctgcgccacg gtcatgcggc gcttgccctc
     4141 cacgcggaag ggcttggtga ccacgcccac gacgagcgcg ttgtgctcct tcgctatctc
     4201 cgccaccacc ggcgccgcgc cggtccccgt cccgccgccc atgccggcgg tgatgaagac
     4261 catgtcggcc cctttgagcg ccttgctgat cttgtcgcgg tcctcgttgg cggccccgcg
     4321 gccgacctcg gggtccgccc ccgcgcccag tcccttggtg agcttcgtcc cgatctggat
     4381 cttcgtctgc gcgagcgagt ccttgagctg ctgcgcgtcc gtgttcgcga cgatgaactc
     4441 gatgccatcc atgccggtgg cgatcattcg gttgaccgcg ttggttcccg cgccacccac
     4501 gccaatcacc ttgattgtcg tgtgaaacgg ccgttcctct tcgagcttaa acatagcccc
     4561 ccctattgta acgatgtatg cgcgtcacac tattacaatg agacatgtat agattatgtc
     4621 tcattatttt tcaaaacaaa aattattttt tctacaaata ttcttcaaac cagcgccgga
     4681 tgcgcgatcc catcgcgccg atgccgccgc ccgccttttt ccgggacgcg cggccctgcg
     4741 agtactggtt catcttcacg ccgtacttca acaggcccac cgcgttcgcg aactgcggcg
     4801 tggccaccac gtccttgagt cccacgatgt cgcgcggcac gccgaggcgc accggcatgt
     4861 tgagcacgcg ctcggccgcc tccacgcagc cctcgatcat gctgccgccg cccacgaaca
     4921 ccgcgccggc cgccacgatg tccttcttgc cgctgcggac gagctcctgc tccaccatct
     4981 ccacgatctc catcatgcgc ggctcgatga tctgggcgag ctcctgcttg aacagccgcc
     5041 gcggcgcccg cccgcccacg gacggcacct cgatggtctc cgaggcgtcc accaggtcta
     5101 tcaccgcgca gccgtacttc cgcttgatcg actccgcgga ctcgatgggc gtccgcaggc
     5161 cgatggagat gtcgttggtc acgtggatcc cgccgatcgc cagcaccgac gagtaggcta
     5221 cgccgccctc gacgtaaatc atgatatcgg tggtcccgcc gccgatgtcc accagcacca
     5281 cgccgagctc ccgctcgtcc tgcgacaggg tcgcctcggc cgacgccagt ggcgagaaga
     5341 cgatatcgct caccggatag cccgccttgt tcacggactt caccaggttc tggatgcagg
     5401 tcgtcccgcc ggtcacgatg tggacctccg cctccagccg cacgccgctc atgccgatgg
     5461 ggtccttgat ccccgtctgg tcgtccacgg agaactcctt ggacagcacg tggacgatct
     5521 cgcggtcggc ggggatcacc accgcctgcg ccgcctcgat cacgcgcttc acctcgaccg
     5581 gggtgatggt gcggttccgg ttggataccg ccaccacgcc gcgcgagttt tcccccttca
     5641 tgtgctggcc ggtgatgccc acgtacatgc cgcccacttc gctcccggcc atgagctcgg
     5701 cgtcctcgat cgccttgccg atcgagctga tggtattgtc gatgttgatg atgacgccgt
     5761 tcttgagccc cttcgccggc gccacgccca cgccgctcac ctcgacatcg ccgttctcgt
     5821 tgcgatagcc gatgaccgcg caggttttgg tcgttccgat atcgagcccg acaataacgt
     5881 cttccattcc gccgcctatt tcatgacccc gaaggtgtcg aaaatgttca tgtgcgcggg
     5941 atacctgccc gccgcgtcca gaaagccgac gcaggtgttg agccgttcaa gggccgcccg
     6001 gttcatctcc gcaagaaact cggtccggcg accgcgaagt cggatccgca ccttgccatc
     6061 gctcctgata tatacttcgg tcagttcacg gcaaaccttc aaaccgcttt tttcggcctc
     6121 gagaatggtc cgcaggtagt cgcggacctg tttcgagagc gatccgctgt gcgcgaaggc
     6181 ccacgggacc acgaccagcg ggcggtcggc cgcatgcacg cggttgtacg agagcgccgc
     6241 aagctcgccg tcgagctcga tgaaggcgta ggcgccctct ttctcgatcg ccatcaccag
     6301 cgccgggcgt ttctcctcga ccgtcaccgt gatccgccgg tccttctcga cgatacggta
     6361 gtccttgacg tacaggttct tccggagcgc cgcttccagc gaatcgacat cgatcacgat
     6421 cccgtcctgc tccaccacgg tgtctgcggc gctgatgagc tcgtacttgg tcaggtattt
     6481 cagccccttg aactcgatgg cgctcacctt cttcacgttc cgaaaggcgt gggcgcggcc
     6541 ggtcgccgcg aacagcatca gcgcgcccag catcatcacc gcaaccatgt tgatccgcgt
     6601 gcgcatcatc acctcgcacc cagggcctcg acgatctgcc ggccggcctt atggatgtcc
     6661 cccgccccca gcgtgaggac gatatcgccc tcgcgcgcgg ctgcggccag cgtcggcgcg
     6721 atctcctcga agcggccgat gattcgcacc tcgcgcgtgt cgtgcttcct gatcgaatcg
     6781 cggatgagcg cgctcgagac gccctcgatg ggctcctccc ccgccggata gatctccgtg
     6841 agaaagagct catcggcgtc ggcgaaggcc tgcccgaact cgtcccagag gagctgcgtc
     6901 cgcgtgtacc ggtgcggctg gaacaccacc acgaggcgcc gcccgagggc cttgagcccc
     6961 gcgagcgtcg cgcggatctc cgtgggatgg tgcccgtagt cgtcgatcac gcgcacgccg
     7021 cgcgcctccc cgacgcgctc catgcggcgc cccactcccg cgaactccgc caatcccgcg
     7081 cggatggccg cgaaatcgag ccccaggtcg agcgccacca ccaccaccgc gagcgaattg
     7141 atcacgttat ggttgccgat gaggccgatc tcgatggcgc ccaggagctc gttcttgtag
     7201 tagcagtcgt agtgcgtccg gccgtccgcc aggcggacgt tcgccgcgcg gaagtcggcg
     7261 ttctccgaaa agccgtaggt gagatacgga cgctccacgc gcggcagcag ctccgcgatg
     7321 acgttatcgt cgatgcacag gacggagtat ccgtaaaacg gaacattgtt catgtaggtg
     7381 agaaaggcct ccttgagccc ctcgaagaac ttgtagtgat cgaggtggtc ggcgtcgatg
     7441 ttggtgacca ccgcgatcgt cggcaacagc ttcaggaacg acccgtccga ctcgtcggcc
     7501 tcgaagacga tgtactcgcc ctcgcccgcg cgcgcattgg agccgaagtt gagcaccttc
     7561 cccccgacca cggcggtggg gttgatgccg ccgtgcgaga gcacgaacgc gaggagcgac
     7621 gaggtggtcg tcttcccgtg cgtcccggcg atgccgatgc cgtgcttgag gcgcaccagc
     7681 tcggccagca tctcggcgcg gtggatgacg ggtatgccgc gcgagcgcgc ctcgatgagc
     7741 tcgggattgt tcttgccgat ggcgctggag gtgaccacca cgctgtagtc cccgatattc
     7801 gacgggaagt ggccgatgaa gaccgtcgcg cccaggtcgc gcaggcgccg cgtctgctcg
     7861 gactcgtgca ggtccgagcc cgaaacctgg aagcccaggg tgatgaggat ctcggcgatc
     7921 ccgctcatgc cgatcccgcc gattcccatg aaatgcatcg ttttcgattt tctgaacacg
     7981 cccctacctc aacgccgtta tgatttcgtc gacgatggtc ttcgccgcgt ccggccgggc
     8041 gaccttgagc gccttctcgg ccatcttcgc cagccgcatg ggattgccca gaaggtccag
     8101 gatgatcggt cccgcgatct ccggcgtggc atcgtcgttt gcgatcttca ccgccgcgcc
     8161 ggcctcgacg aactcgtcgg cgttcttgtc ctggtggttc gccgccgcga agggatacgg
     8221 gatcatcacg gacgggagcc ccatcgcggc gatctccatc atcaccccgg agcctgcgcg
     8281 gctgatggcg atgtcgctcg cgcggtacgc cgcccccacg ttctcgatga acggcgacat
     8341 gtacaccgac cccttctcga gggcgcgccc gctcttctcc cggtactcat tatacgaaag
     8401 gtctccggtg ctccagatga cgccggtatc ggcgaactcg ccggaaaatt tcgtcttgag
     8461 ccccagcatg agctcgttca ggcgcagcgc gccctggctc ccgccgatca acaaaatcac
     8521 gcgcttgcag tggcccaggt tgaacgcccg gcgcgcgtcc tcgcagctga tgggcgcgaa
     8581 cacgcgctgg cggatgggat tgcccgcctg gacgatcttc acgccgtttt tcaaatattc
     8641 gtccgaggcg gcgaaggtcg tgaagatggc cgcggcgcgt ttcgcgaaat accgcgtcac
     8701 cttgccgggc accgaattct gctcgcagag atacaccgga acgcccatgc gcgcggcggc
     8761 cagcagcgcc gggccggaca cgtagccgcc catgccgatc accgcggcga tctgcgagcg
     8821 ggcgatgaag cgcttcgccc agaaataggc gtggaggaac ttcagcgcga cgatgggcag
     8881 catgacggga tttttcgtca gtgacggcgc gccgtagagg agcaggtccg cgtccttcac
     8941 gtccgccagc gacgagaagc gggcatcgcg ccggcccacc aggacgaagg cgtgcacgcc
     9001 gcgtttcacg aactcctcat agatcgcgat gccggggctg atatgacccc ccgtcccgcc
     9061 gccgacgatg agaatgtttt tgcctttcat tgccacacct cttcagtgag ctggacgccc
     9121 tcgtacgccc cggcccggta tcgcgaaatg ttaagcagaa tccccgccgc cgacatgtta
     9181 cacaccagcg acgacccgcc atagctgata aacggcagcg gcaacccggt ggttggcagc
     9241 gccccgctca ccaccccgat gttgatgaac gcctgcacga cgatatagag cgtgagaccg
     9301 accgcgagca gccggccgta ctcgtccggc gcacccagcg agatgatgag cccccgccag
     9361 aagaccgtgc agaagagcgc gatgataagc aacgtcccta aaataccggt ctcctcggcg
     9421 atcaccgcga agatgaagtc cgtgtgcggc tcgggcaagc ggctgatctt ctgggtgccg
     9481 taccccagtc ccgtgcccaa aaagccgccc ttcttgaagg cgataaagga ctgcaccacg
     9541 tgatagccct tgccgaagcg gtcggcccag ggatcgaaat aggagagcag gcggttcttg
     9601 cgataactca cctcgtagat gagcaaatag aacatgggca cgctcacgat ccccactgcc
     9661 gcgatataca ggatgggaaa gcccgagacg aacagaatca gggcggcgat caagaggatg
     9721 tcgatggcgg tcccgaaatc gggctgcagc atgaggagca cgaaggcgag tgcgacaatg
     9781 gccgcaggca ccaggagctg cgccacgtga ttggccaccc cggtccgctc gctggagaac
     9841 accttcacaa gatagagcac caggcacagc ttgacgatct cggatggctg aatctgcaag
     9901 aaccccaggc tgagccagcg cgcggacccc ttggcgctct tgccgatgac cggcacgaac
     9961 accaagatca gcaggacgaa cgagacgagc agcaatatct tcgtatagcg cgcataccgg
    10021 cggtaatcga cctcctggaa cacgagaagc gccccgagcc ccagcacgaa ccacacgatc
    10081 tggcgcttga ggaaatagaa ggaatcctga tagatccgcg cggcgtggat ggcgctcgcg
    10141 ctgtagctca tggcgatgcc gatcccgacc agcaggagca ccgtgacgaa gagaaagacg
    10201 tccgggtcgc cccgccgcct cgtgtcgatg atcgacttca ggtccacgcg atctcccccc
    10261 gaacgagccg ctgaaacgtt tcgcggaaac ggtcgccccg ctcctcgtaa ttcctgaaca
    10321 tgtcgaacga ggcggtggcc ggcgagtaga gcaccgcgtc gcccggcgcg ctgtgccgca
    10381 tcgcctgcgc cagcgcctcc tcgagcgtgg ccaccgggac cggatcgtgg gcggcgaaca
    10441 ggcgcgcgaa ctccggcgtc gattcgccga tgagcagcag gcgcttcacg cgctgagcga
    10501 tgggcccgtt caaccgcgcg taatcgtcgc ccttggtgcg cccgcccagg atgagcaccg
    10561 ccggccgcgt gaggctggcc agcgccatct ccacggcgtt cacggtggtc gccttcgagt
    10621 cgttgatgaa ggtgcgcccc tcgaactcgc ccacgatctc catgcggtgc ggcaggcctt
    10681 tgaatgaata gatcgcctca gcgatcgcgc cgtcatccgc cggccgtccc atccgcgcga
    10741 ggacggcgcg caccatgagg atcgccgcca tcgcgttttg cacattgtgc acgccgatga
    10801 tgcgcatccg cgttgggtcc agcaccggcg cgtcgactac ggccaggcgc accatcgaac
    10861 cgtcgaagcg gatcgcggcg tccgtatcac ggacggcaaa gccgcacgtt cgggcgacga
    10921 ccgcgccgag ccgcgagcgg atcagagcgt catcggcgaa atggacgaag acgtcatcgg
    10981 gacgctggtt catggcgatg cgcatcttcg cgtccacata ctcgtccatc gtcgcgtagc
    11041 ggtcaaggtg gtccggggcg atattcagga tcgcggccac gtccggccga aagcggtcga
    11101 tcgtctcgag ttggaacgac gagagctcga ggaccgtgac cgacgagtcg cgcgtccgat
    11161 cgacgaacga cacgaagggc acgccgatat tgccgcccac gaaggtgtcg atcccgagcg
    11221 ccgcgaggat gtggcccacg agactggtcg tcgtcgtctt cccgtcggtg ccggtgatcg
    11281 cgacgatcaa tcccttcgcg aaacgatagg cgagctcgat ctcggacacg acggggacac
    11341 cccgctcgac agcggccgcg accagcggga tcgcgcgcgg aacgccgggg ctcagcaccg
    11401 ccagatcgaa cccctcgtcg aggagctccg gccgctgagc gccggcgatc acccgcacgc
    11461 cgcggtcaag cgccgcgagc gcgtctgtga gggcggcccg gtccttgctg tcggaaacga
    11521 cgacggcgta tcccttgcgc gcgagaaagt tcgaggcggc cacgccggtg cgcggcccca
    11581 gcccgatgat gagaatcttt tgtatcttcg atagatccat agtatgctaa aaaccctgtc
    11641 ccctgcccct atcccaatcg atacggagcg atggggcgcc cttcccccta acgaagggag
    11701 aggacgggag ggggtatcac aaaatcttca acgaactcaa tcccaggatc gccaggatga
    11761 tgcccaggat ccagaatcga acgaccacct tctgctcggt ccatcccgaa agctcgaagt
    11821 gatgatggat gggcgccatc ttgaacaccc gcttgcccgt gagcttgaac gacaggacct
    11881 gtatgatcac cgacatcgcc tcgacgacga acacgccgcc gacgataaaa agaaagattt
    11941 cctttttaat catgatcgcg acgatcccga tcacgccgcc cagcgcgagc gaacccgtgt
    12001 cgcccatgaa gaccgtcgcg gggttggagt tgaaccacag gaagcccagc gacgcaccgc
    12061 agagcgccga gaggaacacc gtaagctcgg ccccgtcggg gatatagggg atcttgagat
    12121 aggacgcgat cttgacgtga cccgaaagat agcacatgac ggcgagggag gccgcgacga
    12181 acagcaccgt ccccaccgcg agcccgtcga gcccgtcggt gaggttcacg gcgttggagc
    12241 tgcccaccac cacgatcatc gcgaagatca cccaggccca ggtcatgtcc agcgcgatcc
    12301 cgccgatcgc cacgcgcgac agcgtgacca ggggttcgtt cacgaaggga acgtagatct
    12361 gcgtgagcgc ctcgggattg gcggggaaga cgagcatccc tgcgaccacg ccgccggccg
    12421 tcgcgagctg caccacgagc ttgagcttcc ccggcacccc gcccttgcgc ttgcggatcg
    12481 acttgatgta atcgtcggcg aacccgagcg agcccaagac gagcgtcgcg acggtgagca
    12541 gtatcaaata ttcgctattg aaatttcccc acaggaagat cgagatgagc acggcgccca
    12601 ggatgatgac gccgcccatc gtgggcgtgc cggccttcgc cttgtggctc tggggaccgt
    12661 cggagcggat ctcctctttg aagcgcaggc ggcgcagcca cgcgatcacg accgcgccga
    12721 agatgagcac gatcaccagc gccgtgagcg cggcgtatgc ggaacggaac gaaatatact
    12781 ggaagaggcg aagaaacgag aggtattcct gaagcggcaa tataaaatga taaaacatgg
    12841 ttcccttagc ggagcaggtc cgccaccacc tcctccatct tcatcgagcg cgaccctttc
    12901 accaggacca cgtcgtcctt cgcgacccgc ttcttgagcg cgtcgaccag cgactgtttg
    12961 tcggcgaagt gcgccacgcg gtcgccgttc atccccgcct cgcgggcccc cgcgaccgta
    13021 tgcgcggcga gctcgcccca ggcgaagagc agctcgaaac cgctggcggc gatctcgcga
    13081 cccgcctccg catgatagcg cgcggccttc gcgcccagct ccttcatgtc ggaaaccacc
    13141 gcgatcaggc ggcggcccga aaacacccgc gccgccgagg cgaaggcgct tcgcgacgag
    13201 agcgggttcg agttataggt gtcgttgatg acgacgaatt cgccccggac aatctggctg
    13261 cgcttgtcga tcgcgtccat cgatgcgagc gcgcgggcca tcgcggcgtc gtcgatgccc
    13321 aggcaacgcc ccacggcgat cgcggccagg aggttcgcgg cattgtgcac gccgtagagc
    13381 ggcgccgcga acgtcacgcc gcccaggacg atctcggtgc gatcgggctg cagggaatac
    13441 gactcgggcc ggagatcggc gtcggcgtcg agcgcgaagg taagcatgcg cagccccgcg
    13501 tcccgcgcgg cgtcctcgag aatgccaaca cacgccgtct cgcggttcag gatgatggtg
    13561 ccgcccggcc gcatgccgcg gacgatctcc gccttcgcgc gcgcaacgtt ctccacgctc
    13621 cccaggaact ccaggtgccc ctcgcccaca ttggtaatga gcgcgatctc cggccgcacg
    13681 atgcgcgaga gccgctcgat ctcgcccgcg tggttcatgc ccatttccat caccgcgcac
    13741 tcgtgctcgg gacgaatccc caacagcgcg aagggcacgc cgatctcgtt attgtagttc
    13801 ttctcgtttt tatgcgttcg gagcgcggtc gaggcgatcg cgtagaccag ctccttcgtg
    13861 gtggtcttcc cgttggttcc ggtgatgccc accatccgcg cggaggcgga atcccggtgc
    13921 cgcgcggcga tccgtcccag cgcgcgaagc gtatcgtcgc acaggaccgc cgggacgccg
    13981 tggcgttcgg ccgtcgcgcg gtcggactcg cgcattgaaa cgaagcccgc gatcgtccgt
    14041 tccgctgcga gcgtttcgat gaaatcatgc ccgtcgaacc gttctccgac gagcggaacg
    14101 aacaggctct cgggacccag gtcgcgcgaa tcggagctga tggtgcggat gcgcgtatcc
    14161 ggcataccgc cgacaagcga gccgccggcg tacgccgcca tcgtccccat cgttgtcggg
    14221 aaatcggccc tcatgccgcc tcgcgctcgg cgatgaattt gcgcgccatc tcgcggtcgt
    14281 cgaaatgggt cttcgccctg cccacgatct ggtaatcctc gtgccccttc ccggcgatca
    14341 cgacgatgtc gcccgcggag gccatcgcga tccccgcggc gatcgcgcgc gcgcggtcgg
    14401 gctcaacctc gtaggcgcgg ccgtcgatgc ccgcgcggat gtcctcgatg atccgcgccg
    14461 ggtcctccgt gcgcgggttg tcgctggtca cgatcacgtg gtcggagagc tccgcggcgg
    14521 cgcggcccat cagcggtcgc ttcgcccggt cgcggtcgcc gccgcagccg aagacggtaa
    14581 tgagtcggcg cggccggagc tcgcgcaccg acgtgaggag ctttcgcagc gcatcgtcgg
    14641 tgtgcgcgta atcgaccacc acgtggaagc cgagccgcga ggcaatggtg ttgaaccgcc
    14701 cggggatgtg cgcgatcccg gcgagcgcgt cgcggatgca gtcgaatgag atcccgagcc
    14761 ggtgcgccgt cgcgacggcg gcaagggaat tgtacacatg gaagcggccg gcaagcggca
    14821 gtgcgatgcg gccgccccca tcggggcgcg aaagcgtata ggcgatgccc tcgagcgaat
    14881 tgacgacgct tgccggatca acgcggtagg cggcggcgtc gtgcatgccg aaccccagca
    14941 cgggatacga cgccccggcg aagtcgcgcg cgaggcggcg gccgtagtcg tcatcgacat
    15001 tgacgatgcc cgcacggtcg ccgggcaacc cgcccagaag cgtaaagagg cgcttcttcg
    15061 cggcgaagta atcctcgaag tccgcgtgga aatcgagatg gtcgcgcgtg aggttcgtga
    15121 agatggcggc cgtgaaggcg acatggtcca cccgcccgag cgcaaggccg tgcgaggaga
    15181 cctccatgat gagcgtatcc acgccgtcgt cgcgcatctc ggcgatgagc tgctgcaggt
    15241 cgcgcgattc cggggtggtg tttggcgcgg ccgtctccct cccgcaccag cggtagttca
    15301 cggtgccaat cacgccgggg acgcgccccg ctgcgcgcat gatcgcctcg agcatgtagg
    15361 tgatggacgt cttgccgttg gtgccggtca cgccgatgac cgtcatcccc gcgctcgggt
    15421 tgtcatagaa ggcggccgac agggccgaga gcgcgcgtcg cgtattgtcc gaaccgatga
    15481 tagcaacgcc gcgctccgcg aggggcgcga actcaggcaa ccgcgcgcgc gacaccaccg
    15541 ccgccgccgc gcccgcatcg accgccgccg tgacgaaccg gtgcccgtcg gactggaagc
    15601 cctccacggc cacgaagagc gagccgttcg cgatcctgcg cgaatcgtag tccaccgatg
    15661 cgatgggcgc gtcggggaca tcgcccgcga cctggagcgg ctcgtcgatt ccggtgatga
    15721 gttcgcgcag gcgtttcacg ttcgtcccca caggatcact tgcttttcat attcttcggc
    15781 gcgatggcct gaaaatcgtc cggcccgatg ggccgcatgc cgctcccccg ggcataggat
    15841 tcgatcgcgt ccatccgccg gaagcgttcg atctgatagc gcaatcggtc gtttcggtcc
    15901 attagctggc gctccttccg aatgat
//
LOCUS       DAORHF010000058        15860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2786_length_15860_cov_6.055679, whole genome shotgun sequence.
ACCESSION   DAORHF010000058 DAORHF010000000
VERSION     DAORHF010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15860
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2786_length_15860_cov_6.055679"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PKJ16_06965"
     CDS             <1..599
                     /locus_tag="PKJ16_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV46765.1"
                     /translation="LWAYTMLGVHFVRREIVHRERIAPGQPYIIIANHQSLFDILALV
                     TTLGIQYRWVIKREVLKVPLFGYALYASRNIFVDRSNTQKAIASIHRGLERLPEGASV
                     LIFAEGTRSRDSSIREFKKGGFQMAIARHIPILPVAVNGSRHVLPKDSFLIRPGRIRV
                     AVGEPIDTTGCNEEAIPALMERTRSALLALYDPDYGSR"
     gene            complement(607..2100)
                     /locus_tag="PKJ16_06970"
     CDS             complement(607..2100)
                     /locus_tag="PKJ16_06970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HNV46766.1"
                     /translation="MKIDTLIAVDAGCTDEDLTFLGGFVPERLASIASEITGGAVYYA
                     LPPDYRGRLAADPGALVREDREDGAFWRALFGKSDAEHIIRLHADAAFLDPDAIREMI
                     ELQTSYCAEFTYSENLPAGFSCEIVSRALVETLPETEQKTLPLAEVVRANINQFDVEL
                     HYREPDIRDTRISFRASNRRDRRIMENLFRIDNRIPAYGEVRGRIEKNPGALYVGPSY
                     VELELTGRCPLACIFCYRKTLPAERPDMDMGTLQAVLAGMRGFDLPYAVCLGGSGEPL
                     EHARAFEAMDLVLAEPLVSALVIETNGVRADENFAAYLAAKADSRVKVIVNCNGLDAE
                     TYRAAHGADEFARVHRNVIAMRDAVAGVPALSGLVHLQVMKINETEPFLDRYYNFWEK
                     TGIPIILQKQNTYLGALVDRRYADLTPLERTPCWHLQRDLVVFADGTVAFCKQDIAGA
                     NARGSLAASSLAELWEAGRQSFLDEYAKRYPRQPDCRACDEWYTFNL"
     gene            complement(2109..3104)
                     /locus_tag="PKJ16_06975"
     CDS             complement(2109..3104)
                     /locus_tag="PKJ16_06975"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV46767.1"
                     /translation="MKMTLFLTALLSAVLLDASYATTKLPGAETEWQVIDRAVAVVNN
                     RPILESTLNRRFARVRRHKAIANNRLAFEKSRLLDAMIDEQLVVQKAADDSIIVSDEK
                     INGNVEKIMERMKIGTLDDFKKRVVAREGISWEEYREEMRVQLIKEQVMSISIGISPP
                     SEKDATDWYNKNKDKLGFQVNIKHILLRPRNDSMAEEKRINELAKEIAARAQAGESFE
                     NLARRYSQDPVSAGKGGDLSWVLLAELDPYFASQVYQMGRIGQVSGPIRSSYGYHVVK
                     FFGRRVTPFDAVRDRILQLLYHQNLDMQFSRWIAQRRRESEVKIYLQEYVKVPGR"
     gene            complement(3117..3920)
                     /gene="murI"
                     /locus_tag="PKJ16_06980"
     CDS             complement(3117..3920)
                     /gene="murI"
                     /locus_tag="PKJ16_06980"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HNV46768.1"
                     /translation="MDDRPIGVFDSGVGGLTVCAAVRDRLPGEHIIYFGDTGRFPYGT
                     RSGETIIRYSLEIADYLVRRNVKMIVIACNTASAVALDTLRSRVPVPVMGVIGAGARA
                     ACGRMGGGAVGVIGTRATVASGSYVKAVQAVRPGTAVLQQHASLLVSLVEEGWTDDEV
                     TRLAARRYIDGLYDAGVRTLILGCTHYPLLKGAIRAVYPDLDLVDIGVEIALEVQAVL
                     REKNLENAGLSGTIELYASDVTDTMQRLKNLFFGDDGSVLRKLVISGAD"
     gene            complement(3932..5524)
                     /gene="cimA"
                     /locus_tag="PKJ16_06985"
     CDS             complement(3932..5524)
                     /gene="cimA"
                     /locus_tag="PKJ16_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013748805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HNV46769.1"
                     /translation="MTAPRMRDITLYDTTLRDGAQTVGVSFSLQDKLRITAELDRLGI
                     DYIEGGWPGSNPKDEAYFREVRSLALGHARVVAFGSTRRPGTRCADDPQLRALVESGA
                     QTVTIVAKTWDFHVTAALGISLDENLDLVGESIAFLREHGREVFLDAEHFFDGHRDNP
                     EYALSVLDRAREAGAAMLVLCDTNGGTLPADAERITREACARCDVPIGVHFHNDAGVA
                     VANSILAVQAGAVSVQGTMNGYGERCGNCDLAAAIPNLVLKMGYACAVRERLAHLTEV
                     SRFVSETANLAHNERAPYVGDSAFAHKGGIHVSALGKDTRTYEHIDPSLVGNRRRVLV
                     SELSGKSNIEMKAAEFGIALGKDVTLSKEVLRRIKCLEDKGFQFEAAEGSFELLLRES
                     IGEYRPFFKLLGFRVITEKRGAEDVTCEATIKVEVDGRERHTAANGNGPVAALDNALR
                     KALVSFFPDIEQLQLSDYKVRVLNEKQGTGAAVRVLIDHKRQAEHWGTVGVSANIIEA
                     SWHALVDGIEYMLFKGARRAGS"
     gene            5659..6828
                     /locus_tag="PKJ16_06990"
     CDS             5659..6828
                     /locus_tag="PKJ16_06990"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46770.1"
                     /translation="MRAPSPMNRNQQHNDAEPIDSIRRRLAHKGFIVFPAVGLGLAAA
                     GAGAAAYAVPAREAAVIVVLIALSALALAGTLAFVIASRRRISDALAKIEEELSWKGI
                     LMEHSNELVVILNPYGQITAVNRNVADLLHLERGAIIGKPFREIVYDEKLDRNLRLND
                     MILRKLGEVFSGRETDLTCFSKNRGTNEIVTINYRLVPIFTNERFEHILAVGRVIQGD
                     LLSSRYLMREFEYYRLDNNISLVFQLCMRLTRNLEGRLPKNQVLMAQVGLQEVLINSI
                     EHGNLEIDYHMKTRLKSREGNYWELLVQECDQDHLRERKVHVSYTLEPDRVVYTIRDE
                     GAGFDWRRFLERTAEDIDQELMANLHGLGIQFARTVFDISFNEAGNEVHLSKSFS"
     gene            6867..7289
                     /locus_tag="PKJ16_06995"
     CDS             6867..7289
                     /locus_tag="PKJ16_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002790493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV46771.1"
                     /translation="MEAHYRNPAPTVDIIIEIEDAATSALRGIVLIERKNPPHGWAIP
                     GGFVDYGESVEHAAEREALEETSLRVRLVRQFHVYSDPARDPRQHTISTVFVARAQGE
                     PAGADDALTARIFAIDALPAPLVFDHAQILADYREGRY"
     gene            7292..9472
                     /locus_tag="PKJ16_07000"
     CDS             7292..9472
                     /locus_tag="PKJ16_07000"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV46772.1"
                     /translation="MELSNFFLRLTGLAIDTLMNTTRANIRWHDAENIPDQPVVYVVN
                     HFTRMETFFLPHLIYKITGKHALSLAYHEFFGGGFGRYLSKLGAISTKAENRDTLMTS
                     ALLRGDFPCIIFPEGQMVKDKKIVEKGKFMVYNSGIRRPPHTGAALLALRSEFVRRHM
                     RRLAETGNAAGVSAYRAHFEIPASFRTDEITARATVIVPVNITYFPVRSRKNLLNTIA
                     GKIAGAVPSRVDEELEVEGAMILDGVDIDINFGAPIGLDEYFGDPAIVRALESAEPVI
                     GRDETARRLPFARASTALMARYMSEIYALTTVNHDHIFSYLLTAYWRKRIRIEDFRMR
                     AFYAANLLYGYRSTIRSFHSTLMRDQSHLLSDDTFHKLDNFLTAVAADGLIRLKNGFI
                     IRNRARFRLQHEFHSVRKDNIVEVMRNEIEPIVDAQRCVRRASWLPGFLLRRRIRDQF
                     VALDRDIFERDYRHFFREGESKPREVGAPFFLRRFFSRTGVLLVHGYMAAPEEVRPLA
                     DYLRTKGYAVYGVRLRGHGTTPDDLAQRTWVDWYESVNRGYIIMKNTVRRMAIVGFST
                     GAGLALLHAANKPHTVRCAVSINAPLKLANPASRFAFLFAFLNRLLARFKIPRALLPF
                     VPNNPENPDINYARNPVAGVDQLEELMGIVNDRLRDITMPVLVVQGSDDPVVKAESGK
                     KIYKKIKARKKELYVVPANHHGILRGDEAPELFVRVHRFLRRHL"
     gene            complement(9479..10825)
                     /locus_tag="PKJ16_07005"
     CDS             complement(9479..10825)
                     /locus_tag="PKJ16_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46773.1"
                     /translation="MTRRLLAGVLALAIVGMPSLARAMGKSFAMGGVQSVLSDGAYDA
                     AKNPALLPLHTEGAAAGLIVRSLAGYSFTGSSYVYFQDSERGITAEMRDVMLTLYGVD
                     LCFLARIGGSVVGVSVTDGSDPQYMRVTSNNTIAISNAIVETKGDDTTYAPALNLSWG
                     MTISAHTSIGLILSGKHTGKHSVEGKSMMNEGRELYYITKTTDTSTTSASLGFGLLYR
                     TDDAQAGIAVLSGDVTWIGQTGGYDYADKRYLINPSIEDRSGSEDALVTSSGVYTKGP
                     MIVAGGYRRLSPMFAVAVEGSYVFRNSYRSRAVTVSDPNSNGECAVTEIAVITRQSGT
                     TGFRGGVEFTPLTGLSFMLGGAYSYERTGSDTPSSSDRTNSAYQTTGKESLTATGGVV
                     YSSPQRFSVGIIAAYGDTVIRERKREYSGNGDLTSRSTSRMSLRLTMVGLSSQFIF"
     gene            complement(10822..13260)
                     /gene="lon"
                     /locus_tag="PKJ16_07010"
     CDS             complement(10822..13260)
                     /gene="lon"
                     /locus_tag="PKJ16_07010"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV46774.1"
                     /translation="MTLDDIIPDESDLQVETNLFDSGLVLVDQVLPSQLYIIPIRFRP
                     IFPGIVTPLIISHGRFSEAIDKVVGSSRTVGLVLLKDDDSEEIMSADLYRHGTAVSIL
                     KKLNLPDGGINVLINSIKRFRVKKYISEKPHLVADVEYLADDMPRKNMEIKALTRAVL
                     SQLKMLSENNPLFTEEMKLTMVNVEEPGKIADFVTSILNIEKKEFQDVLETLNVKKRL
                     EKVLKLLHKEMGVLSLQKKIQGQINEKIEKQQREFFLKEQLKAIKTELGLDEDERSAE
                     IKRLRKKAEEMDLRGEPGERIREELEKLSIMDTSSSEYSITRNYIETVLSLPWHARSD
                     DAIDIAKAERILDRDHYGLADVKKRILEFLAVRKLKPDSRGSIICLVGPPGVGKTSLG
                     QSIAAAMNRKFFRMSLGGMRDEAEIKGHRRTYVGAMTGKIMSGLKIVKTRNPVFMLDE
                     IDKLSASFQGDPAAALLEVLDPEQNREFRDHYLDVPFDLSDVFFVTTANTLDSIPEVL
                     ADRMEVIRLSGYITEEKYEIARRYLLPKQLSRHGLKKDAIRIAKKEFLYLINNWAREA
                     GVRNLERQIEKLCRKTATMMARGARNIRHDLTLEMIRGHLGQELYPADELLGADRPGM
                     AVGLAWTSLGGTTMVVESIGVGRSGTEGLQLTGQLGAVMVESANIAFSYVQHLLREDE
                     RARSFFAQNKVHIHVPEGATPKDGPSAGITMATSLFSLVTGRIIKPRLAMTGELTLSG
                     RVLPIGGVKEKVVAARRAGIREIVLPRLNAKDLKEIPAYIKKGIAFHEVDDIAEVIEL
                     AFPGKGARRARRVS"
     gene            complement(13345..14364)
                     /locus_tag="PKJ16_07015"
     CDS             complement(13345..14364)
                     /locus_tag="PKJ16_07015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HNV46775.1"
                     /translation="MAPSSRTEALGRLDGDDELVRAILPHCRLTRGGVWEDPVRGHRV
                     GVCDAADPDEVARLFADVRVRTVINDPPYNVIVGGVKKSDALGKMTLAAYLEFSRRWV
                     ENAVACMADDADLFVWLGADYRDHFRPLPDFMVLMRAVEALRAKNYITVRNQRGYGTQ
                     KNWMWIRQELLHYAKGNPSFRVVYTDIPKVLKGYYKVVGGAMTENIERSRSETIRPGN
                     VWIDIQQVFYRMEENVPGCYAQKPLSAIERIMRCCSDEGDVIGDFFAHSGSTLIAGER
                     LGRRVITCDLDPVFAEIAIRRLERFRATGKAGWQWQSPFPEVTGDATESDGGDAADPD
                     DGRLF"
     gene            complement(14372..14995)
                     /locus_tag="PKJ16_07020"
     CDS             complement(14372..14995)
                     /locus_tag="PKJ16_07020"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HNV46776.1"
                     /translation="MTSGSNNRARVQRTTAAGIAFGMIHLCVVVAISAQLYSERPSLA
                     RSRDATPGAALEALFASAHFDDCLVAGRAFEITAYCPGPCCNTGTVIRDGAPVTVDWS
                     GRVAASRVSLDLFRAAGIDVVAVDPASVPFGSLVRSEGRLYLALDRGSAIVGDRLDVL
                     LSDHASTVRFGRRRDRPAIVFTPARPDAVLRAVESFALRGTPFPQGG"
     gene            15206..>15860
                     /locus_tag="PKJ16_07025"
     CDS             15206..>15860
                     /locus_tag="PKJ16_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46777.1"
                     /translation="MVLNIITLAACVVLILIFRRLDRSNLKLIKLKRYSDKIQKDFKK
                     LTENEGRRLDDATIEMDILLKKAAAVSVGLKESIIDIEGKLKGLSIEKSNLKKVEEDL
                     RVVSQAARDVNQQIEFISATRESFSEVTKRIGVLNENIANLDSSTAAIIESFNNKVRE
                     RSRELTAEISLQTGKLKESTRDIEERLRDFALILEDTRDKNAAETRAEIEQMGIKIQN
                     "
BASE COUNT         2817 a         5286 c         4941 g         2816 t
ORIGIN      
        1 agctgtgggc ctataccatg ctgggcgtcc acttcgtgcg ccgggagatc gtccaccggg
       61 aacggatcgc cccgggccag ccgtatatca tcatcgcgaa ccaccaatcg ctcttcgaca
      121 tcctggcgct ggtgacgacg ctgggaatcc aataccgctg ggtcatcaag cgggaggtcc
      181 tgaaggtccc gctcttcgga tacgccctgt acgcctcgcg aaacatcttc gtcgaccgtt
      241 cgaacacgca gaaggccatc gccagcatac atcgcggtct ggaacgcctg cccgaaggcg
      301 ccagcgtgct tattttcgcc gagggcacgc gctcccggga cagcagcatc cgcgaattca
      361 agaagggcgg cttccagatg gcgatcgcgc gccacatccc cattttaccg gtggcggtga
      421 atggcagccg gcatgtcctg ccgaaggatt cgttcctgat acggccggga aggatacggg
      481 tggcggtcgg cgagcccatc gatacgacgg gctgcaacga agaagcgatc cccgcactca
      541 tggagcggac gcggtcggcg ctgctcgcgc tctacgaccc ggactacgga tcgcgataga
      601 tccgccctag aggttaaagg tgtaccactc gtcgcaggcg cggcagtcgg gctggcgggg
      661 atagcgcttc gcgtattcat ctaaaaacga ctgccgcccg gcctcccaga gctcggcgag
      721 cgacgacgcg gcaagcgatc cccgcgcgtt cgcgcccgcg atgtcctgct tacagaaggc
      781 gaccgttccg tcggcgaaca cgacgagatc gcgctggagg tgccagcagg gggtccgttc
      841 gagcggcgtg agatcggcgt agcgccggtc gacgagtgcg cccagatagg tgttctgctt
      901 ctggagaatg atgggaatgc cggtcttctc ccaaaaattg taatagcggt cgagaaacgg
      961 ctccgtttcg ttaatcttca tgacctgcag gtgcacgagc ccggagaggg ccggtacgcc
     1021 cgcgaccgcg tcgcgcatgg cgatgacatt gcggtggacg cgcgcgaact cgtcggcgcc
     1081 gtgcgccgcg cggtaggtct cggcgtcgag cccattgcag ttcacgatca ccttcacgcg
     1141 cgaatcggcc ttcgcggcga ggtaggccgc gaaattctca tcggcacgga cgccgttcgt
     1201 ctcgatgacc agcgcgctca cgagcggctc ggcaagcacg agatccatcg cctcgaaggc
     1261 gcgcgcgtgc tcgagcggct cgcccgatcc ccccaggcac accgcgtacg ggagatcgaa
     1321 accccgcatg cccgcaagca ccgcctgaag ggtgcccatg tccatgtccg ggcgctcggc
     1381 cgggagcgtt tttcgatagc agaagataca cgctagcggg cagcggccgg tgagctcgag
     1441 ctcaacgtac gagggcccga catagagcgc gccggggttt ttctcgatac gcccgcgcac
     1501 ctcgccatag gcgggaatgc ggttgtcgat gcggaacagg ttttccatga tgcgccggtc
     1561 gcggcggttc gacgcccgaa acgagatgcg cgtgtcgcgg atgtcgggct cgcggtagtg
     1621 cagctccacg tcgaactggt tgatgttcgc gcgcactacc tccgccagcg ggagcgtttt
     1681 ctgctcggtc tccggcagcg tctccaccag cgcgcgcgac acgatctcgc acgagaagcc
     1741 ggcgggcagg ttctccgaat aggtgaactc ggcgcagtag gaggtctgga gctcgatcat
     1801 ctcgcggatg gcgtcgggat cgaggaaggc ggcgtcggca tgaagccgta tgatgtgctc
     1861 ggcgtcggac ttcccgaaga gcgcgcgcca gaaagcgccg tcctcccggt cctcacgcac
     1921 gagggccccg ggatcggccg cgaggcgccc gcggtagtcc ggcgggagcg catagtacac
     1981 cgcgccgccg gtgatttcgc tcgcgatcga cgcgagcctc tcgggaacga acccgccgag
     2041 aaaggtgagg tcctcgtcgg tgcatcctgc gtcaaccgcg atgagcgtgt cgatcttcac
     2101 tgggccgcct acctcccggg caccttgacg tactcctgca gatatatctt cacctccgat
     2161 tcgcgccgac gctgcgcgat ccagcgggaa aattgcatgt cgaggttttg atgatagagc
     2221 aactgcaata tgcggtcgcg cacggcgtcg aagggcgtga cgcggcgccc gaagaacttc
     2281 accacgtgat agccgtagct ggagcggatg ggcccggaga cctggccgat gcgtcccatc
     2341 tggtacacct gcgaggcgaa atacgggtcg agctcggcca agagcaccca tgaaaggtcg
     2401 ccgcccttcc ccgcggaaac gggatcctgc gaatagcgcc gcgcgaggtt ttcgaaggat
     2461 tcgccggcct gcgcgcgcgc cgcgatctcc ttcgccagct cattgatgcg cttctcctcg
     2521 gccatggagt cgttgcgggg tcgcagcagg atatgcttga tgttcacctg gaagccgagc
     2581 ttgtccttgt tcttgttgta ccagtcggtc gcgtccttct ccgagggcgg cgagatgccg
     2641 atggagatcg acatcacctg ctccttgatg agctgcaccc gcatctcctc gcggtactcc
     2701 tcccaggata tcccctcgcg cgccaccacg cgcttcttga aatcgtccag cgtgccgatc
     2761 ttcatccgct ccatgatctt ttcgacattg ccgttgatct tctcgtcgct gacgatgatg
     2821 gagtcgtcgg ccgccttctg gaccacgagc tgctcgtcga tcatggcgtc caggagccgg
     2881 cttttctcga aggcgagccg gttgttggcg atggccttat ggcgccggac gcgcgcgaac
     2941 cggcgattga gcgtgctctc gagaatgggc cggttgttca ccacggcgac ggcgcggtcg
     3001 atgacctgcc actcggtttc ggcgccgggg agcttcgtcg ttgcatacga cgcgtccagc
     3061 aggaccgccg acagcagcgc ggtcaggaat aatgtcattt tcattcgccc cacctgttag
     3121 tccgctcccg atatcaccag cttgcgcagc accgacccat catcgccgaa aaagaggttt
     3181 ttgagccgct gcatggtatc ggtcacgtcg gacgcataga gctcaatggt gcccgaaagc
     3241 ccggcgtttt caaggttttt ttcccgcaaa acggcctgca cctccagggc gatctccacg
     3301 ccgatgtcca ccaggtcaag gtcgggatat accgcgcgaa tcgccccctt gagcagggga
     3361 tagtgcgtgc agccgaggat gagggtgcgc acccccgcgt cgtagagccc gtcgatgtac
     3421 cgccgcgccg cgagtcgcgt cacctcgtcg tcggtccagc cctcctcgac gagcgacacc
     3481 agcagggacg cgtgctgctg caacaccgcg gtccccgggc gcacggcctg tacggccttc
     3541 acgtatgatc cgctcgccac cgtcgcgcgc gtcccgatca cgccaaccgc gccgccgccc
     3601 atccggccgc aggccgcgcg cgcgcccgcg ccgatcacgc ccatcaccgg gaccgggacg
     3661 cggctgcgca atgtgtcgag cgccacggcc gacgcggtat tgcaggcgat cacgatcatc
     3721 ttcacgttcc ggcgcaccag gtagtcggcg atctcgagcg agtagcggat gatggtctcg
     3781 ccggagcgcg tcccgtaggg gaagcgcccg gtatcgccga aatagatgat gtgctctccc
     3841 ggcaggcggt cgcggaccgc cgcgcatacg gtgagccccc ccacgccgga atcgaatacg
     3901 ccgatgggac gatcgtccac gcgcgcctcc ctcacgatcc cgcgcgccgc gcgcccttga
     3961 acagcatgta ctcgatgccg tccaccagcg cgtgccacga ggcctcgatg atgttcgcgg
     4021 aaacgcccac cgtgccccag tgttcggcct gccgcttgtg gtcgatgagc acgcgcaccg
     4081 ccgcgccggt cccctgcttt tcattgagca cgcgcacctt gtagtcggag agctggagct
     4141 gctcgatgtc gggaaagaac gagaccagcg ccttgcgcaa cgcattgtcg agcgccgcga
     4201 ccgggccgtt gccgttggcg gcggtgtgcc gctcgcggcc gtccacctcg accttgatgg
     4261 tggcctcgca ggtgacgtcc tccgctccgc gcttctcggt gatcacgcgg aaccccagca
     4321 gcttgaagaa cggccggtac tcgccgatcg actcgcgcaa caggagctcg aaagagccct
     4381 cggccgcctc gaactggaac cccttgtcct cgaggcactt gatgcgccgg agcacttcct
     4441 tcgacagcgt gacgtccttg cccagcgcga tgccgaactc ggcggccttc atctcgatat
     4501 tgctttttcc cgaaagctcg gagacgagca cccggcggcg gttgcccacc agggacggat
     4561 cgatatgctc gtaggtgcgc gtgtccttgc ccagcgcgga gacgtggatg ccgcccttgt
     4621 gcgcgaaggc gctgtcgccc acgtacgggg cgcgctcgtt gtgggcgaga ttggccgtct
     4681 cgctcacgaa gcggctcacc tccgtgaggt gcgcaagccg ctcgcgcacc gcgcaggcat
     4741 agcccatctt caaaaccagg ttggggatcg cggcggcgag gtcgcaattg ccgcagcgct
     4801 cgccgtaccc gttcatggtc ccctgtaccg acaccgcgcc ggcctgcacc gcgaggatgg
     4861 aattggccac ggcgaccccg gcgtcgttat ggaaatgaac gccgatcggg acgtcacagc
     4921 gcgcgcacgc ctcgcgggtg atccgctccg cgtcggcggg cagggtgccg ccgttggtgt
     4981 cgcacagcac cagcatggcg gcgccggcct cccgcgcgcg atcgagcacg gaaagcgcgt
     5041 attcgggatt gtcgcgatgg ccgtcgaaga agtgctcggc gtcgagaaag acctcgcggc
     5101 cgtgttctcg caaaaaggcg atcgactcgc ccaccaggtc caggttctcg tcaagggaga
     5161 tccccagcgc ggcggttacg tgaaagtccc aggtctttgc gacgatcgtt acggtttggg
     5221 cgccggattc caccagcgcg cgcagctgcg gatcgtccgc gcaacgcgtc cccggccgcc
     5281 gcgtggaacc gaaggcgacc acgcgggcat gcccgagcgc gagcgaacgc acctcccgga
     5341 aataggcctc gtccttcggg ttcgatccgg gccagccgcc ctcgatataa tcgatcccca
     5401 gccggtcgag ctcggcggtg atgcgcagct tgtcctgcag cgaaaacgac acgccgaccg
     5461 tctgcgcgcc gtcgcgcagg gtcgtgtcgt agagggtgat gtcgcgcatg cggggcgctg
     5521 tcatggtgta ttatcgggtg tctgctggct cgtccggtcg agcggtcccg ggaagagtga
     5581 acggtgatga cgaccggtta tatttcaagt aatttttcgc ttgctttttt tcccgtcgcg
     5641 aggcgaggaa attgcacgat gcgcgctccc tcacccatga accggaacca gcagcacaac
     5701 gacgccgagc cgatcgactc cattagacgc cgcctcgcgc acaaaggttt catcgttttt
     5761 ccggcggtgg ggctcgggct tgccgcagcg ggcgcaggcg ccgccgcata cgccgtcccg
     5821 gcgcgcgagg cggccgtcat cgtcgtgctg atcgcgctct cggcgctggc gctcgccggg
     5881 acgctcgcgt tcgtcatcgc gtcgcgccgc cgcatcagcg atgcgctcgc caaaatcgag
     5941 gaggagcttt cctggaaggg gatcctgatg gagcactcca acgagctggt ggtcatcctc
     6001 aatccctacg gccagatcac ggccgtcaac cggaacgtgg ccgacctgct gcacctcgag
     6061 cgcggcgcca tcatcggcaa gccgtttcgc gagatcgtct acgacgaaaa gctggaccgg
     6121 aacctccgcc tcaatgacat gattttgcga aagctcggcg aggtcttctc cgggcgcgag
     6181 accgatctca cctgcttctc caaaaaccgc ggcaccaacg agatcgtgac catcaactac
     6241 cgtctcgtcc ccatcttcac caacgagcgc ttcgagcaca tcctggcggt ggggcgcgtg
     6301 atccagggcg acctcctgag ctcgcgctat ctcatgcgcg agttcgagta ctaccggctg
     6361 gacaacaaca tctccctcgt cttccagctc tgcatgcgcc tcacgcgaaa cctcgagggg
     6421 cggctcccca agaaccaggt gctcatggcg caggtgggcc tccaggaggt cctcatcaat
     6481 tcgatcgagc acggcaacct cgagatcgac taccacatga aaacgcggct caagtcgcgc
     6541 gagggcaact actgggagct gctcgtgcag gagtgcgacc aggaccacct gcgcgagcgc
     6601 aaggtgcacg tctcctacac cctcgagccc gaccgggtcg tctacacgat ccgcgacgag
     6661 ggcgccggct tcgactggcg gcgctttctc gagcgcacgg ccgaggacat cgaccaggag
     6721 ctcatggcga acctccacgg gctgggcatc cagttcgcgc gcaccgtgtt cgacatatcg
     6781 ttcaatgagg cgggcaatga ggtacacttg agcaaatcat tctcgtgagc gccgggaacc
     6841 caaccgcccg gcaaggagaa gagcgcatgg aagcgcacta ccggaatcca gcaccgacgg
     6901 tcgacatcat catcgagatc gaggacgccg cgacgagcgc actccgcggc atcgtcctca
     6961 tcgaacggaa aaacccgccg cacggctggg cgatcccggg cggcttcgtc gattacggcg
     7021 agtccgtgga gcatgccgcc gagcgcgagg cgctggagga gacctcgctc cgggtccgcc
     7081 tggtgcgcca gttccacgtc tattccgatc ccgcgcgcga cccgcgccag cacaccatct
     7141 cgacggtgtt cgtcgcgcgc gcccagggcg agcccgccgg cgccgacgac gcgcttaccg
     7201 cgcgcatctt cgccatcgac gcgctccccg cgcccctcgt cttcgaccac gcgcagatcc
     7261 tcgcagacta ccgggagggg cgctactgac gatggagctc tcgaatttct tcctccgcct
     7321 gacgggcctg gccatcgaca cgctcatgaa caccacgcgc gccaacatcc gctggcacga
     7381 tgcggagaac atcccggatc agcccgtggt gtacgtggtg aatcacttca cgcggatgga
     7441 gacctttttc ctgccgcacc tgatctacaa gatcacgggc aagcatgccc tctcgctggc
     7501 ctatcacgag ttcttcggcg gcggcttcgg gcgctacctc tcgaagctgg gcgcgatctc
     7561 caccaaggcg gagaaccgcg acacgctcat gaccagcgcc ctcctgcgcg gcgatttccc
     7621 ctgcatcatc ttccccgagg gacagatggt caaggacaag aagatcgtcg agaagggaaa
     7681 gttcatggtg tacaactcgg ggatccgccg cccgccgcac acgggcgccg ccctcctggc
     7741 cctgcgctca gagttcgtgc ggcgccacat gcgccgcctc gccgagacgg ggaatgcggc
     7801 gggggtgtcc gcctatcgcg cgcacttcga gattcccgcc tcgttcagga ccgacgaaat
     7861 caccgcgcgc gccaccgtca tcgtgccggt gaacatcacc tactttccgg tgcgctcgcg
     7921 aaagaacctc ctgaatacga tcgccggcaa aatcgcgggc gcggtcccgt cgcgcgtgga
     7981 cgaggagctc gaggtcgagg gggcgatgat cctcgacggg gtggacatcg acatcaactt
     8041 cggcgcgccc atcggcctgg acgaatactt cggcgatccc gccatcgtcc gggctctcga
     8101 atcggcggag ccggtcattg gacgcgacga gaccgcccgg cggctcccct tcgcgcgcgc
     8161 aagcacggcg ctcatggcgc gctacatgtc ggagatctac gcgctgacca ccgtgaacca
     8221 cgatcacatc ttctcctatc tgctcaccgc ctactggcga aagcggatca ggatagagga
     8281 tttccgcatg cgcgcctttt acgcggccaa cctgctctac gggtaccggt ccaccatccg
     8341 ctcgtttcac tcgaccctga tgcgcgacca gtcccatctg ctctcggacg acacctttca
     8401 caagctcgac aacttcctga cggcggtcgc cgccgacggc ctgatccgtc tgaagaacgg
     8461 cttcatcatc aggaaccgcg cgcggttccg ccttcagcac gagttccact ccgtccgcaa
     8521 ggacaatatc gtggaggtga tgcgcaacga gatcgaaccc atcgtggacg cgcagcggtg
     8581 cgtgcgccgc gcaagctggc ttccgggctt tctgctgcgt cggcgcattc gcgatcagtt
     8641 tgttgcgctc gaccgcgaca tcttcgagcg cgattaccgg cattttttcc gcgaaggcga
     8701 gtccaagccc cgggaggtcg gcgcgccgtt tttcctccgt cgcttttttt cgcgaacggg
     8761 cgtcctgctc gtgcacggct acatggcggc gccggaggag gtgcgccccc tcgccgacta
     8821 tctccgcacc aagggctacg cggtctacgg cgtgcgcctc cgggggcacg gcaccacgcc
     8881 tgacgacctc gcgcagcgga cctgggtcga ctggtacgaa tccgtcaacc gcggctatat
     8941 catcatgaaa aacaccgtgc gccgcatggc gatcgtcggg ttctcgacgg gcgcggggct
     9001 ggcgctcttg cacgcggcga acaagccgca caccgtgcgc tgcgccgtct cgatcaacgc
     9061 gccgctcaag ctggccaatc ccgcgtcgcg cttcgcgttc ctcttcgcct ttctcaaccg
     9121 cctgctcgcg cgtttcaaga ttccgcgcgc gctcttgccg tttgtgccca acaacccgga
     9181 gaaccctgat atcaattacg cgcgcaaccc cgtggccggc gtggaccagc tcgaggagct
     9241 gatgggaatc gtgaacgacc ggctgcggga catcaccatg ccggtgctgg tggtgcaggg
     9301 ctcggacgat ccggtggtga aggcggagag cggcaagaag atttacaaga agatcaaggc
     9361 ccgaaagaag gagctctacg tggtcccggc gaaccatcac ggcatcctgc ggggcgacga
     9421 ggcgccggag ctctttgtca gggtgcaccg ctttttacgg cggcacttgt agcagcgact
     9481 agaaaatgaa ttgggagctc aggcccacca tggtcagccg caagctcatc cgtgacgtgc
     9541 tgcgactggt caaatccccg ttgcccgaat actcccgttt tcgttcccga atcaccgtat
     9601 cgccgtaggc cgcgatgatg ccgacggaaa agcgctgcgg gctcgaatac accacgccgc
     9661 cggtcgcggt gagcgactcc ttgcctgtgg tctggtaggc gctgttcgta cggtccgagc
     9721 tcgacggcgt gtcgctcccg gtgcgttcgt aactgtaggc gccgcccagc atgaacgaaa
     9781 gccccgtgag gggcgtgaac tcgacgccgc cccgaaagcc ggtcgtgccg ctctgtcggg
     9841 ttatgacggc aatctccgtc accgcgcatt cgccgttcga attcggatct gagaccgtaa
     9901 cggcgcgcga ccgatacgaa ttgcgaaata cgtagcttcc ctcgacggcc acggcgaaca
     9961 tcggcgagag ccgccggtat ccgccggcga caatcattgg ccccttcgta tacaccccgc
    10021 tgcttgtgac cagcgcgtct tccgatccgc tccggtcctc gatcgacgga tttatcagat
    10081 agcgcttatc ggcataatca tacccgccgg tctggccgat ccacgtgaca tcgccactca
    10141 gcacggcaat gcccgcctgc gcatcgtccg tgcgatagag caggccaaac ccgagggacg
    10201 cggacgtggt cgaggtgtcg gtcgttttcg tgatgtaata cagctcccgg ccttcattca
    10261 tcatgctctt gccctcgacc gaatgctttc cggtgtgctt cccggaaagg atcaggccga
    10321 tggacgtatg cgccgaaatc gtcatccccc aggacaggtt gagcgcgggg gcgtaggtcg
    10381 tatcatcccc ctttgtttcc acaatcgcat tactaatagc gatggtgtta ttactcgtta
    10441 cccgcatata ttgcgggtcg ctcccatcgg tgaccgatac gcccacaacg gaaccgccga
    10501 tccgcgccag gaaacacagg tcgacgccat acagggttaa catcacatcc cgcatctcgg
    10561 cggtgatacc gcgttcgctg tcctggaaat agacatagct ggagccggta aacgaatatc
    10621 ccgcgaggga acgcacaata aggccagcag cggcgccttc cgtatgcagc ggcaggaggg
    10681 cgggattctt tgcggcgtcg taggcgccgt cggagagcac ggattggacg ccgcccatcg
    10741 cgaagctctt tcccatcgcc cgcgcgagcg acggcatgcc cacaatcgcg agtgcgagaa
    10801 cgccggcgag cagacgacgc gtcacgacac cctccgcgca cgccgcgcgc ccttcccggg
    10861 gaaggcgagc tcgatcacct cggcgatatc atcgacctcg tgaaatgcga tccccttctt
    10921 gatgtaggcg ggtatctcct tgaggtcctt ggcgttgagc cggggcagga cgatctccct
    10981 gatgcccgcg cggcgcgcag ccaccacctt ctccttcacg ccgccgatgg gcagcacccg
    11041 ccccgagagc gtgagctcgc cggtcatcgc caggcggggc ttgatgattc gtcccgtgac
    11101 cagcgaaaag agcgatgtgg ccatggtgat gcccgccgag ggcccgtcct tgggcgtggc
    11161 cccctcgggc acgtggatgt ggaccttgtt ctgcgcgaaa aaggagcgtg cgcgctcgtc
    11221 ctctcgcagc aggtgctgca cgtagctgaa cgcgatgttc gccgattcga ccatcaccgc
    11281 gccgagctgg ccggtgagct gcagtccctc ggtgccggag cgtcccacgc cgatggactc
    11341 caccaccatg gtcgtcccgc cgagcgaggt ccacgccagc cccaccgcca tgccgggccg
    11401 gtcggccccg aggagctcgt cagccggata gagctcctgg cccaggtgcc cgcggatcat
    11461 ctccagcgtg agatcgtgcc ggatattccg ggcgccgcgc gccatcatcg tcgcggtctt
    11521 gcggcacagc ttctcgatct gccgctcgag attacgcacg cccgcctcgc gcgcccagtt
    11581 gttgatcaga tagaggaatt ccttcttggc gatgcggatg gcgtccttct tgagcccgtg
    11641 acgcgagagc tgcttgggca ggagatagcg ccgggcgatc tcgtacttct cctcggtgat
    11701 gtagccggag aggcggatca cctccatgcg gtcggccagc acctcgggga tgctgtcgag
    11761 cgtgttcgcg gtggtgacga agaagacgtc cgacaggtcg aagggcacgt ccagatagtg
    11821 gtcgcggaac tcgcggttct gttcggggtc cagcacctcc aggagcgccg cggcgggatc
    11881 gccctgaaag ctcgccgaga gcttgtcgat ctcgtcgagc atgaagacgg ggttgcgggt
    11941 cttcacgatc ttcagcccgc tcatgatctt cccggtcatc gccccgacat aggtgcgccg
    12001 gtgccctttg atctcggcct cgtcgcgcat cccgcccagc gacatgcgga agaacttccg
    12061 gttcatcgcc gcggcgatgg actgcccgag ggaggtcttg cccacgccgg gcgggcccac
    12121 caggcaaatg atggagccgc gcgaatcggg cttgagcttg cgcacggcca aaaactccag
    12181 gatgcgcttc ttcacgtccg ccaggccgta gtggtcgcgg tccaggatcc gctcggcctt
    12241 cgcgatgtcg atggcgtcgt ccgagcgcgc gtgccacggg agcgagagca ccgtctcgat
    12301 gtagttgcgc gtgatggagt actcggacga ggaggtgtcc atgatcgaaa gcttctcgag
    12361 ctcctcgcgg atccgctcgc cgggctcgcc gcgcaggtcc atctcctcgg cctttttccg
    12421 caaccgcttg atctcggccg agcgctcgtc ctcgtccagg cccaactcgg tcttgatggc
    12481 cttgagctgc tccttgagga aaaactcgcg ctgctgtttc tcgatcttct cgttgatctg
    12541 cccctgtatc ttcttctgga gcgagagcac tcccatctcc ttgtgcagga gcttgagcac
    12601 cttctcgagg cgcttcttca cgttgagcgt ctccagaacg tcctggaatt ccttcttttc
    12661 gatattgaga atggaggtga cgaagtcggc gatcttgccc ggctcctcca cgttcaccat
    12721 ggtcagcttc atctcctcgg tgaacagcgg attgttctcc gagagcatct tgagctgcga
    12781 gagcaccgcg cgggtgagcg ccttgatctc catgttcttg cgcggcatgt cgtcggccag
    12841 gtactccaca tcggccacca ggtgcggctt ctcggagatg tacttcttca cccggaagcg
    12901 cttgatgctg ttgatgagca cgttgatccc gccgtcgggc aggttcagct tcttgaggat
    12961 gctcacggcc gtgccgtgcc ggtacaggtc ggccgacatg atctcctcgg agtcgtcgtc
    13021 cttcaggagc accagcccca cggtgcgcga ggaccccacc accttgtcga tggcctcgga
    13081 gaaacgcccg tgcgaaatga tcagcggcgt gacgatcccc ggaaagatgg gccggaagcg
    13141 gatggggatg atatagagct gcgagggcag gacctggtcg acgagcacga ggccgctgtc
    13201 gaagaggttg gtctcgacct gcagatcgct ttcgtcggga atgatgtcgt ccagtgtcat
    13261 gccatgtcct cgcgcgcccc gcggacgggg cggtagtatc cgtccctgag cctgtgcgga
    13321 caacagggag ctgtcaagat taattcagaa gagccgaccg tcgtcgggat cggccgcgtc
    13381 gccgccgtcg gattccgtcg catcgcccgt cacctcgggg aaggggctct gccattgcca
    13441 gccggccttc ccggtcgcgc ggaagcgctc gagacggcgg atcgcgatct ccgcaaacac
    13501 gggatcgagg tcgcacgtaa tcacgcgccg ccccagccgc tcgcccgcga tgagggtgct
    13561 cccggagtgc gcgaagaaat cgccgatcac gtcgccctcg tcgctgcagc agcgcatgat
    13621 ccgctcgatc gcggaaagcg gcttctgcgc atagcagccg ggcacgttct cctccatgcg
    13681 gtagaagacc tgctggatgt cgatccacac gttcccgggc cggatggtct ccgagcggct
    13741 gcgctcgatg ttttccgtca tcgcgccgcc caccaccttg tagtagccct tgagcacctt
    13801 ggggatgtcg gtgtacacca cgcgaaacga gggattcccc ttcgcgtaat gcaagagctc
    13861 ctggcggatc cacatccagt tcttctgcgt cccgtagccg cgttggttgc gcacggtgat
    13921 gtagttcttc gcgcgaagcg cctccactgc acgcatcagc accatgaaat cgggcagcgg
    13981 ccgaaagtgg tcccggtaat cggcgcccag ccagacgaac aggtcggcat cgtcggccat
    14041 gcaggccacg gcgttttcga cccagcgccg ggaaaactcg agataggccg cgagggtcat
    14101 ctttcccagc gcgtcgcttt ttttcacccc gcccacaatc acattgtacg ggggatcgtt
    14161 gatgacggtg cgcacacgga cgtccgcgaa gagacgcgcg acctcgtcgg gatcggccgc
    14221 gtcgcacacg cccacgcggt ggccgcgcac cgggtcctcc caaacgccgc cgcgcgtgag
    14281 acgacagtgc ggcaggatcg cgcgaacgag ctcgtcgtcg ccgtccagcc gccccagcgc
    14341 ctcggtgcgg gatgatggcg ccatcgccgc ctcacccgcc ctgcgggaac ggggtaccgc
    14401 ggagcgcgaa ggactcgacc gcccgcagca ccgcatccgg gcgcgcgggc gtgaacacga
    14461 tcgccggccg gtcgcgccgc cgcccgaagc ggaccgtcga tgcgtgatcg gacaagagca
    14521 cgtcgagccg gtcgccgacg atggcgctcc cccggtccag cgcgagatag agccggccct
    14581 cggagcgcac gagcgaaccg aacggcaccg acgcggggtc caccgcgacg acatcgatgc
    14641 cggcggcgcg gaagaggtcc agcgacacgc ggcttgccgc gacgcgtccg ctccagtcga
    14701 ccgtgacggg cgcgccgtcg cggatcaccg tcccggtgtt gcagcacggg cccgggcaat
    14761 acgcggtaat ctcgaatgcg cgcccggcca ccaggcagtc gtcgaagtgc gcgctcgcga
    14821 agagcgcctc gagcgccgcg ccgggtgtcg cgtcacgcga acgcgcgagc gacggccgct
    14881 cggaatacag ctgcgcggag atggcgacga cgacacatag atgtatcatg ccgaaggcga
    14941 tgccggcggc cgtcgtccgt tgcacgcgcg cgcggttgtt cgaaccggaa gtcatggtgc
    15001 cgccattgcc gattcctcga accgatttgt caacatcata tccccgccgt tcccgcgccg
    15061 gcgtggtgcg ccatgtatta atataatgtg acataaaatt ttattgacat atttgttatg
    15121 tctcatcaat ataaacattt gcgggtatat attcactaaa gacgatccct tcacgagcac
    15181 acgggcgcga acccggagag acgatatggt cctgaacatc atcaccctgg ccgcatgcgt
    15241 tgtcctcatt ctcatcttcc ggcggctcga ccgctcgaac ctgaagctca tcaagctcaa
    15301 gcgctactcc gacaagatcc agaaggactt caagaagctc accgagaacg aggggcggcg
    15361 gctcgacgac gccaccatcg agatggacat cctgcttaaa aaggccgccg ccgtttccgt
    15421 ggggctcaag gagtccatca tcgacatcga ggggaagctc aaggggctct ccatcgagaa
    15481 atccaacctc aagaaggtcg aggaggacct gcgcgtcgtg tcgcaggccg cgcgcgacgt
    15541 gaaccagcag atcgagttca tctccgccac ccgcgagagc ttctccgagg tgaccaagcg
    15601 catcggcgtg ctcaacgaga acatcgcgaa cctcgattcg agcaccgccg ccatcatcga
    15661 atcgttcaac aacaaggtcc gcgagcgctc gcgcgagctc accgccgaga tcagcctgca
    15721 aaccggcaag ctcaaggaga gcacccgcga catcgaggag cgcctgcgcg atttcgcgct
    15781 catcctggag gacacccgcg acaagaacgc cgccgagacc cgcgccgaga tcgagcagat
    15841 ggggatcaag atccagaacc
//
LOCUS       DAORHF010000059        15851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2791_length_15851_cov_5.539567, whole genome shotgun sequence.
ACCESSION   DAORHF010000059 DAORHF010000000
VERSION     DAORHF010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15851
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_15851_cov_5.539567"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PKJ16_07030"
     CDS             complement(<1..318)
                     /locus_tag="PKJ16_07030"
                     /inference="COORDINATES: protein motif:HMM:NF033919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2779 family protein"
                     /protein_id="HNV46778.1"
                     /translation="MKRLRTPLFVVPITLYLLVLFTAPGVAGMVGSALSDGTSATSVE
                     RDSRVRSVQKALENKIVIEKLRAHGLDEGEIRAKLSGLSDEQLHLLAQASDRALAGGD
                     VIVI"
     gene            complement(439..1266)
                     /locus_tag="PKJ16_07035"
     CDS             complement(439..1266)
                     /locus_tag="PKJ16_07035"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV46779.1"
                     /translation="MKAGILLRYVILAGSFPVIALICWADAQFDPQVSLSLLYLIPVA
                     LVAWFFGRIAGIGIGFTAIVSEAYATLSVVNPQAYMSIELWNSFGRMAFYALIVILLN
                     YIATLLEKERLSARKDYLTGLNNSRSFYELAEIEIDRSRRSKLPITIAYFDVDGFKQV
                     NDRMGHPEGDRLLKAIASALGACVRRIDMAARLGGDEFAVLLPLDDFPAAQAAIARIR
                     ERLLDTVRGTWPVTFSVGAITYAAPPSTVSEMVTAADELMYGVKKSGKNDIRHITKD"
     gene            complement(1277..2218)
                     /locus_tag="PKJ16_07040"
     CDS             complement(1277..2218)
                     /locus_tag="PKJ16_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV46780.1"
                     /translation="MTEKRILVTGGAGFLGSHLCDRLVAQGHEVVCVDNFFTGAKRNI
                     AHLLGNPRFELIRHDIIFPLFLEVDEVYNLACPASPVHYQYNAIKTIKTSVMGAINML
                     GLAKRTRATILQASTSEVYGDPAVHPQKETYWGNVNPIGIRSCYDEGKRCAETLFFDY
                     HRQHRLPIKVMRIFNTYGPRMHPDDGRVVSNFIVQALKGEDITVYGDGSQTRSFCYAD
                     DLIEGMIRLMNSRDGLTGPVNIGNPNEFTILQLAEKVIALTGSKSKIIHQPLPQDDPT
                     QRQPNIDIARAELGWEPAVQLAEGLERTIAYFREILR"
     gene            complement(2228..3343)
                     /locus_tag="PKJ16_07045"
     CDS             complement(2228..3343)
                     /locus_tag="PKJ16_07045"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV46781.1"
                     /translation="MNFIDLGAQQKRIRDKIDANIKKVLDSNAYINGPELTELEKKLG
                     EYVGVKYAVGCASGTDALLMPLMALGAGPGDAIFTTPFTFIATAEVVQILRATSVFAD
                     VEPDTFNIDVNKLEDAIKKTKKEGKLRPAGIIPVDIFGACADYDEILALAKQYDMWVI
                     EDACQSFGATYKGKKACSFGIAGATSFFPAKPFGCYGDGGMIFTDDKKMYDDLLSIRV
                     HGQGIDKYNNVRVGINGRLDTIQCAIMLAKYEIFAEEVELRQKVAATYAELLKGVVET
                     PFVKSHNVSAWAQYCVMHPKRDHIMAELGKEKIPTAIYYPKSLHLQEAFVSLGYKKGD
                     MPVSEAIGEKIFALPMHPYLTREDQQTVASAITKAIG"
     gene            complement(3464..4846)
                     /locus_tag="PKJ16_07050"
     CDS             complement(3464..4846)
                     /locus_tag="PKJ16_07050"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HNV46782.1"
                     /translation="MTTPTRIELPANAAQDIRAIVRAIVESGHECYLVGGSVRDLILG
                     RPVYDFDFATDAHPTVVTKLFRRTVPTGIKHGTVTVLIRDRAYEVTTYRADGTYLDGR
                     RPESVTFSKTLREDVARRDFTINGLAYDVLREEIIDYVEGLEDLARRSIRTIGDPLAR
                     FTEDGLRPFRACRFAAALEFGIEPETLDAIPQTLEVARLVAVERVRDELMKMLAAPRP
                     SVGLEYLRVSGLMDLVLPELARCHGVSQNKYHVYDVYYHSLYSCDAAPAERPVIRLAA
                     LLHDLGKIPTRRAGDDGEATFYNHEVVGTRMAKAVMKRLKFPAEDIQKVANLVNNHMF
                     HYTDEWTDGAVRRFMRKVDLENLEDLFALRLADRRGNGQRDGLPAPIRELMRRIDTVI
                     EEENAIKVTDLDINGHVIMQEFGVAPGPIIGKILNELLELVLDDPALNTRDVLVERAR
                     GIFARLREES"
     gene            5180..6700
                     /locus_tag="PKJ16_07055"
     CDS             5180..6700
                     /locus_tag="PKJ16_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HNV46783.1"
                     /translation="MTHAGFDNEKYLEEQTREILERVERLGDKLYLEFGGKLMYDYHA
                     ARVLPGFDPNVKMRLLRNLKSRADIILCIYAGDIERKKVRADFGITYDVDALKLIDEL
                     KEWGIGITGVVITRFENQPAARIFRNKLERQGVRVYTHGFTRGYPTDVDLVVSDEGYG
                     ANDYIETTSPLVVVTGPGPGSGKMATCLSQCYHDHLRGVKSGYAKFETFPIWSLPLKH
                     PVNMAYEAATADIGDFNMLDPFHLEAYDAKAVNYNRDVETFPVLKRILEKIGGAAASY
                     RSPTDMGVNRAGFGIVDDAAVRAAACQEIIRRYFRYSCEYLMGFVGKDTLQRVELLMN
                     ELGIKPEDRAVVEPARRAAAEAEAAQNGKGNAGVFCGAALALPDGTIVTGKNSPQMHA
                     TSSLVLNAIKMRAGIPDGIHLLSPQIIGHINHLKGKVLGERSVSLNLDETLIALSISA
                     ATNPTAQAAMEKLTDLAGCDLHSTHMPTPGDEDGLRKLGVNITSDPQFASRNLFVM"
     gene            6853..7407
                     /locus_tag="PKJ16_07060"
     CDS             6853..7407
                     /locus_tag="PKJ16_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46784.1"
                     /translation="MTNASEITLYSGGHKGAEAEFGRQAERYGMSQVTFSYDGHKMEH
                     AVGVQALGPKELAEGDVSMAIVSQRMRRNFSEVDAIRKVIQSIYHMVNKGFQVFAIGW
                     IQDDNTVKGGTGWAVELARLFNRPVSVYDQDRKGWFTWTGGEWQKDSPVISHKTFCGT
                     GTRNLSGDAVAAIADLYARSFGAR"
     gene            7518..8768
                     /locus_tag="PKJ16_07065"
     CDS             7518..8768
                     /locus_tag="PKJ16_07065"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46785.1"
                     /translation="MGTMMTFPETPGAAVAFFAIYAFFGWLVEVAYRSATQRRFVNAG
                     FLFGPFVPLYGMGALLVLALGSLMAGWHPALVFLAIGVVLTALEYLFGFLSERLFDLT
                     LWDYSANRFNLHGRVCLLYSVAWTALAFGVSVFVHPFVTRFVGWFDEQALRWFAAAFA
                     VYYAIDIAFSVAAVLDFRRRLTFLLANFVTLANPDVERFFDTFRRLLRAFPNLNRQLT
                     ANLSENLKRRIDDLLASVGARIQDELKGRTPAYREYKGIVRDILDHPEFKRLKEFYHH
                     NSSIYEHAVRVSFLSYRICRYLNLDYHSAARGGLLHDFFLYDWRNHDEPDLARDKFHG
                     IHHPRIALENATAHFAINDVERDIIVKHMWPLTLTPPRYRESFVVGCVDKYLASKELL
                     GELPLPGGRRKRPGRARRVRTGPA"
     gene            complement(8811..10466)
                     /locus_tag="PKJ16_07070"
     CDS             complement(8811..10466)
                     /locus_tag="PKJ16_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46786.1"
                     /translation="MTRRRGIIAAALAALALLVADGRAGSIVFTSEGCELLNVIEDTA
                     ANGRRFYRIADITYESSALPEITDCVLSFNRPVVAMGTDDAGHYRIKYASYEPVRGRG
                     IHGAGGAQFFKAQHRVELQSERNLWLGNCEDLGSFSLEFRFRADSLRDESVIFSRIGY
                     ASGKKNGLEIVLKNGRLTARFHGVFRDQSGRPRVVYLNRGRRLKLKAWHHALVSFDRV
                     SGKLSKRINGREDEAVYATESGEPFVNVLRPAFECDDLPAGVVGKDFSGIIDEFRISH
                     RHIADLKRETEIASKRHQRLTTIGREPVNREGVVTGPVHTLPATGTQARLFSWREELS
                     SGTFVWMEFRTSDDLFGQADPEPKWYRIVNNQRDIYRMRVGSEFLRGKYCQWRAHLVP
                     SPDGRRSPRLWDIALEYRVDAPPRVPLMVAVAGAGDKSVTIKWKKNVEEDLCGYKIYY
                     GARPGRYDGIITHSRERRITNALSSTNEIEITVTNHVIEENRARDTRGLLSYPIMKNN
                     VLYFFAVSAYDTYRIDTKYNHESALSAPVTGRPFAGSEIRPEE"
     gene            complement(10463..10927)
                     /gene="rpiB"
                     /locus_tag="PKJ16_07075"
     CDS             complement(10463..10927)
                     /gene="rpiB"
                     /locus_tag="PKJ16_07075"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HNV46787.1"
                     /translation="MKIAIGSDHAGYPLKMLIVKTLPPLVPAAVVTFTDVGTHSEESC
                     DYPDYAAAVAAAVRDGSADLGIAICGTGIGASIAANKVAGVRAALCTNEFMAEMARRH
                     NNATVLVLGARVVGSDLALRIVQRFLESPFEGGRHQRRLDKIDAIERDNRTQ"
     gene            complement(11065..12504)
                     /locus_tag="PKJ16_07080"
     CDS             complement(11065..12504)
                     /locus_tag="PKJ16_07080"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV46788.1"
                     /translation="MEPPRSSAWRELEVQGSLIYLFLVLSVFIVAFAMLMLANVRGQG
                     LITPSVLIATSLTCSLQTYRRKKAGRPAAGYSWAAASIFVMIPIAQKFIYVSNYGWEV
                     ALSSYNSSVQIIIAVVINQFLYDTRLFKVLAVAALAGWLAFLVAAVLSGTPMSIQSVE
                     HGRGVSGFVVLREVFIMLVSIIVWFAVYRIIPLTENYDRTTTEQRRIIESNNTLLEAQ
                     VHERTQELVTINNALRERNVQIESELDMARLILNKLMPEALANIEGLDYFALNVSMDK
                     VGGDFYGFKHAGGPLEILLADASGHGLHAAFLSLITKMTYDSIETQGSPSGTLGELNR
                     AVCRSSVRGNFVTAVCCVVDVANRCVRISSAGHWPVIVFNRDSGVLSELGVHGIPLGW
                     IPEYRFAAVERQLSPGDRVIAFTDGIIECGNARGELFGMERLGDFIRGHAGSTPRELS
                     ESLLSHLHEYTETAAFNDDLSIIVCDVIR"
     gene            12714..13106
                     /locus_tag="PKJ16_07085"
     CDS             12714..13106
                     /locus_tag="PKJ16_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46789.1"
                     /translation="MKRTNARTVPGYVAGIVLAAALAMGVAAHAADFTGTWKTNRGTL
                     QLAQEGNLVIGRFSGEARWRIEGTTSGNRLEFVWADPAGRRGRGVLVAYPGYCSFEGA
                     WWPGSLVGHGGEWRGIKTSDDYFCGGDE"
     gene            13108..13782
                     /locus_tag="PKJ16_07090"
     CDS             13108..13782
                     /locus_tag="PKJ16_07090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HNV46790.1"
                     /translation="MSANGTALVIIDMQRYYLSPASPFYRYFSDLSPGCMDYISKRCA
                     DTTVPAITMLLGAFRAHGAPVVYCRLCGVDPERRDLHRFFRDTYVRARAAGYDNVYPL
                     AGDPLAEIDRRIAPRSGEAVFTKTTFSAFASTDIDARLRARGIDTLVFAGLATSQCVE
                     TTARDASDRGFGVVHVADAQADYSEETHCASLYSSQGVCGGDVVDAAEYLARRGWDRP
                     ADDRRD"
     gene            complement(13779..14243)
                     /locus_tag="PKJ16_07095"
     CDS             complement(13779..14243)
                     /locus_tag="PKJ16_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46791.1"
                     /translation="MNCDKAMRRYLETDNRAYVALAVKFHALRCARCRAEIARLDREF
                     RALLRQAPFDAPPDLADAVMHAARRSRIENATNVGTGKWVAVGGAILSSIVLVPFSDS
                     LIWLKDHFGGHFEVPFSIMLGVAISVYAALYIGTHLEQIKALQDAITRKLRI"
     gene            complement(14230..14796)
                     /locus_tag="PKJ16_07100"
     CDS             complement(14230..14796)
                     /locus_tag="PKJ16_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV46792.1"
                     /translation="MNADTATDEYVVRRVLRGDTEAFRIIITRHQGMVYSIGRRFLRN
                     DDDARDFAQEVLIKAFDKLGTFKGSAPFRAWLARIAYNHGINAVKAARDPGTLAEEAL
                     ESRGATPERSYEHNEIRALLERAVEALPENFRLCLDCYFFYGMPYADICEMTGIPVNT
                     IKSNVFRAKQMLRDALRGTIAEDYHELR"
     gene            complement(14793..15161)
                     /locus_tag="PKJ16_07105"
     CDS             complement(14793..15161)
                     /locus_tag="PKJ16_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013757684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46793.1"
                     /translation="MGGMQNVPSAAAQVIVTIVPIVGIVMGSVVIFFYLLYAHREKML
                     MIRERMYTPKKFDLPVFSLFAGLLLTAIGACLIAFFLIKEGFAYSVLSGMIPFAVGAS
                     LLIFFKLYTSMRGGPSNDAR"
     gene            complement(15175..>15851)
                     /locus_tag="PKJ16_07110"
     CDS             complement(15175..>15851)
                     /locus_tag="PKJ16_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HNV46794.1"
                     /translation="GRLLAETTGDNCDLDVNFVTTIPHADLVAGYKRIIEHIYTPRHY
                     FARGLDLLRRLPRRPVASGATTVRDLRALVRSLFVQTFSSYGHRYLAFLARALWINPR
                     RFALAANLAIKEYHFNEITTGILAADRFAAVLEGAVGRLDAELERMLAKPTPAHPRRI
                     ERRGRAIVAQLRKKYGQLPGDLPESLHAQFAATESRCEERITHWAMRAHLLRQGLRQR
                     VTFDQE"
BASE COUNT         2705 a         4971 c         5276 g         2899 t
ORIGIN      
        1 gatcacgatc acgtcgcccc ccgcaagcgc ccggtcggac gcctgcgcga gcagatggag
       61 ctgttcgtcg gaaagcccgg agagcttcgc gcggatctcg ccctcgtcca ggccgtgcgc
      121 gcgcagcttc tcgatcacga tcttgttctc gagcgccttt tgcacggagc ggacgcggct
      181 gtctcgctcg acgctcgtgg cgctcgtccc gtcgctgagc gccgagccga ccatccccgc
      241 gacccccgga gcggtgaaga ggacgagcag gtacagggtg atgggaacca cgaagagcgg
      301 cgtgcgtaat cgtttcatcg gagacctccg ggatcatcac agtatgcgat ctgctcgtcg
      361 gtcatcatct atgcgcacgc gcgcgggaaa atcaagcaaa aaccgatggg gtgatgataa
      421 aaaaccgcgc gtggtagatc agtccttcgt gatgtgccgg atgtcgttct tgccggattt
      481 cttgacgccg tacatcagct cgtcggcggc cgtgaccatc tccgatacgg tcgacggcgg
      541 cgcggcgtag gtgatcgctc ccacgctgaa ggtgaccggc catgtcccgc gcacggtatc
      601 gaggagccgt tcgcggatgc gcgcgatcgc cgcctgggcc gccgggaaat cgtccaacgg
      661 cagaagaacc gcgaactcgt cgccgcccag gcgcgcggcc atgtcgatcc ggcgcacgca
      721 cgcgcccagc gccgaggcga tcgccttgag cagccggtcg ccctcggggt gtcccatgcg
      781 gtcgttcacc tgcttgaagc cgtccacgtc gaaataggcg atggtaatag gcaacttgct
      841 tcggcgggaa cggtcgatct cgatctctgc gagctcgtaa aaggaccggc tgttatttag
      901 gccggtgaga taatctttac gcgccgaaag ccgctccttt tcgagtaggg tcgcgatgta
      961 attcaggaga atcacgataa gcgcatagaa cgccatccgg ccaaaggagt tccagagttc
     1021 gatgctcatg tacgcctgcg gatttacgac ggaaagggtg gcgtaggcct cggagacgat
     1081 cgcggtgaag ccaatgccga tccccgcgat acgcccgaag aaccaggcga cgagcgcaac
     1141 ggggatcaag tacagaagcg aaagagacac ctgcgggtca aactgggcgt cggcccagca
     1201 gatgagtgcg atgacgggaa aggatcccgc gagaataaca tatcgtagca gaatccccgc
     1261 tttcattgtc ggatcgttag cgcaggatct cccggaagta ggcgatggtg cgctcgagcc
     1321 cctcggcaag ctgcacggcg ggctcccagc cgagctccgc tcgcgcgatg tcgatgttgg
     1381 gctgccgctg cgtgggatcg tcctggggga gcggctgatg gatgatcttc gatttcgagc
     1441 ccgtgagcgc gatcaccttc tcggcgagct ggagtatggt gaactcgttg ggattgccga
     1501 tgttcaccgg ccccgtgagc ccgtcgcggc tgttcatcag ccggatcatc ccctcgatga
     1561 ggtcgtccgc gtagcagaag ctgcgggtct gcgaaccgtc gccgtacacg gtgatgtcct
     1621 cgcccttgag cgcctgcacg atgaagttgc tcaccacgcg gccgtcgtcg gggtgcatgc
     1681 gcggcccgta ggtgttgaag atgcgcatca ccttgatggg aagccggtgc tggcgatggt
     1741 aatcgaagaa gagcgtctcg gcgcagcgct tgccctcgtc gtagcacgag cggatgccga
     1801 tggggttcac gttgccccag taggtttcct tctgcgggtg caccgccggg tcgccgtaga
     1861 cctccgaggt ggacgcctgc aggatcgtgg cgcgcgtgcg cttggccagg cccagcatgt
     1921 tgatggcgcc catcaccgag gtcttgatgg tctttatggc gttgtactgg tagtggacgg
     1981 gcgaggccgg gcaggccagg ttgtagacct cgtccacctc gaggaagagc gggaagatga
     2041 tgtcgtgccg gatgagctcg aagcgcgggt ttcccagcag gtgcgcgata ttgcgcttgg
     2101 cgccggtgaa gaagttgtcc acgcacacga cctcgtggcc ctgcgcgacg agacggtcgc
     2161 acaggtgcga tcccaaaaag cccgcgccgc cggtgacgag gatccgcttc tccgtcatgc
     2221 ccgcccccta gccgatcgct tttgtgatgg cgctggcgac cgtttgctgg tcctcgcgcg
     2281 tgaggtacgg gtgcatgggc agcgcgaaga tcttctcgcc gatcgcctcg ctcaccggca
     2341 tgtctccctt cttgtagccc aggctcacga aggcctcctg caggtggagc gacttgggat
     2401 agtagatcgc ggtggggatc ttctccttgc cgagctcggc catgatgtgg tcgcgcttcg
     2461 ggtgcatcac gcagtactgc gcccaggcgg agacgttgtg cgatttcacg aagggcgttt
     2521 ccacgacgcc cttcaggagc tcggcgtagg tcgcggccac cttctggcgg agctccacct
     2581 cctcggcgaa gatctcgtac ttggccagca tgatggcgca ctggatggtg tccagacggc
     2641 cgttgatgcc cacgcgcacg ttattgtact tgtcgatgcc ctgcccgtgc acgcggatgg
     2701 acaggaggtc gtcgtacatc ttcttgtcgt cggtgaagat catgccgccg tcgccgtagc
     2761 agccgaaggg cttggcgggg aagaagctcg tcgccccggc gatgccgaag gagcaggctt
     2821 ttttaccttt ataggtcgcg ccgaagctct ggcaggcgtc ctcgatcacc cacatgtcgt
     2881 actgcttcgc gagcgcgagg atctcgtcgt agtcggcgca ggcgccgaag atgtccacgg
     2941 ggatgatgcc cgcgggccgg agctttcctt ccttcttggt cttcttgatg gcgtcctcga
     3001 gcttgttcac gtcgatgttg aaggtgtcgg gctcgacgtc ggcgaacacc gaggtcgcgc
     3061 ggaggatctg caccacctcg gccgtcgcga tgaaggtgaa cggcgtggtg aagatggcgt
     3121 cgccggggcc cgcgcccagc gccatgagcg gcatgaggag cgcgtcggtg ccgctcgcgc
     3181 agcccacggc gtatttcacg cccacgtact cgccgagctt cttctccagc tccgtgagtt
     3241 cgggcccgtt gatgtacgcg ttcgagtcga ggaccttctt gatgttcgcg tcgattttat
     3301 cgcggatgcg tttctgctgt gcgccgaggt cgatgaaatt catggtggcg tgctccctgt
     3361 atatggtgtc ggagaattgg acaaatccaa tgaagacggc atttttccgc tggccggata
     3421 aaaatcaaca tatttttgga ggggccgatt gcgggcgcgc ccgctacgac tcctcccgaa
     3481 gccgggcgaa gatcccccgc gcccgctcca ccaggacatc gcgcgtgttg agcgcgggat
     3541 cgtccagcac cagctcgagg agctcgttta agatcttccc gatgatgggg ccgggggcca
     3601 ccccgaactc ctgcatgatg acatgtccgt tgatgtccag gtcggtcacc ttgatcgcgt
     3661 tctcctcctc gatcaccgta tcgatgcggc gcatgagctc gcggatgggg gcggggaggc
     3721 cgtcgcgctg cccgttgccg cgccggtccg ccaggcgcag ggcgaacagg tcctccaggt
     3781 tctccaggtc cacctttcgc atgaagcggc gcacggcgcc gtcggtccac tcgtcggtgt
     3841 agtggaacat gtggttgttc acgaggttcg cgaccttctg gatgtcctcg gcggggaact
     3901 tgaggcgctt catgacggcc ttggccatgc gggtgcccac cacctcgtga ttgtagaagg
     3961 tcgcctcgcc gtcgtcgccc gcgcgccgcg tggggatctt gccaaggtcg tgcaggagcg
     4021 cggcgagccg gatgacgggc cgctccgccg gcgcggcgtc gcaggagtag aggctgtgat
     4081 agtagacgtc gtacacgtgg tacttgttct gcgaaacgcc gtggcagcgc gcgagctcgg
     4141 gcagcaccag gtccatgagg cccgagacac gcaggtactc cagtcccacg gagggccgcg
     4201 gcgcggcgag catcttcatg agctcgtcgc gcacgcgctc cacggcgacc aggcgcgcca
     4261 cctcgagggt ctgcgggatg gcgtcgaggg tctccggctc gatgccgaac tcgagcgcgg
     4321 cggcgaagcg gcaggcgcga aaggggcgca ggccgtcctc ggtgaagcgc gcgagcggat
     4381 cgccgatggt gcggatggaa cggcgcgcca ggtcctcgag accctccacg taatcgatga
     4441 tctcctcgcg cagtacatca tacgccaggc cgttgatggt gaagtcgcgc cgggcgacgt
     4501 cctcgcgcag cgtcttcgaa aaggtgacgc tctccggccg ccgcccgtcc aggtaggtcc
     4561 cgtcggcgcg gtaggtggtc acctcgtagg cgcggtcgcg gatcagcacg gtcaccgtgc
     4621 cgtgcttgat gccggtgggc acggtgcgcc ggaagagttt cgtcacgacc gtcggatggg
     4681 cgtcggtcgc gaagtcgaaa tcgtacaccg gccggccgag gatgagatcg cgcacggagc
     4741 cgcccacgag atagcactcg tggccgctct cgacgatcgc gcgcacgatg gcgcggatgt
     4801 cttgtgcggc gttggccgga agttcgatgc gggtcggcgt ggtcatgcgg ccaaacctat
     4861 ggcgggtgcg gccgaattca atacaatttt tcccgtcgtg cgtcgtgctc attatttcac
     4921 ttgcgatttt ttgttgtgcg ggtgatgctg gttgcatgtg acataaataa ttgatactct
     4981 catgggatga atcatcgcga tgtaggggct gagggccatc acactcgctt tgtcgcgcac
     5041 acccccaccc atcctccccc ttcatacggg ggaggtgtcc gaaggacgga gggggtcgcg
     5101 actccggcgg ttgatccata ccgttgcgct tcacaacgga atccatatcc acaacgctga
     5161 ttttacgaac gaggacgaca tgacgcacgc gggtttcgat aacgaaaaat acctcgagga
     5221 gcagacgcgc gagatcctgg agcgggtgga gcggttgggc gacaagctct acctcgagtt
     5281 cggcgggaag ctcatgtacg attaccacgc ggcgcgggtg ctgcccggtt tcgatcccaa
     5341 cgtgaagatg cgcctgctgc gcaatctcaa gagccgcgcc gacatcatcc tgtgcatcta
     5401 cgccggcgac atcgagcgca aaaaggtgcg cgccgatttc ggcatcacct acgacgtgga
     5461 cgcgctcaag ctcatcgacg aactcaagga gtgggggatc ggcatcaccg gggtggtgat
     5521 cacgcgcttc gagaaccagc ccgcggcgcg catcttccgc aacaagctgg agcgccaggg
     5581 ggtgcgcgtg tacacgcacg gcttcacgcg cggctacccc accgacgtgg atctcgtggt
     5641 gagcgacgag ggctacggcg cgaacgacta catcgagacg acctcgccgc tggtggtggt
     5701 cacggggccc ggtcccggga gcggcaagat ggccacctgc ctctcgcagt gttatcacga
     5761 ccacctgcgc ggcgtgaaga gcggctacgc gaagttcgag acctttccca tctggagcct
     5821 gccgctcaag catcccgtga acatggccta cgaggccgcg accgcggaca tcggcgactt
     5881 caacatgctt gatcccttcc acctggaggc ctatgacgcg aaggcggtga actacaaccg
     5941 cgacgtggag acctttcccg tgctcaagcg catcctcgag aagatcggcg gggcggcggc
     6001 gtcgtaccgt tcgcccaccg acatgggggt gaaccgcgcg ggcttcggca tcgtggacga
     6061 cgccgcggtg cgcgcggccg cctgccagga gatcatccgg cgctattttc gctactcgtg
     6121 cgagtacctc atgggcttcg tggggaagga caccctgcag cgggtggagc tcctgatgaa
     6181 cgagctggga atcaagcccg aggaccgcgc ggtcgtggag cccgcgcgcc gcgcggccgc
     6241 ggaggcggag gcggcgcaga acggcaaggg gaacgcgggc gtcttctgcg gcgccgcgct
     6301 cgcgctgccg gacgggacca tcgtcaccgg caagaactcg ccgcagatgc acgccacctc
     6361 cagcctggtc ctcaacgcta tcaagatgcg cgcggggatc cccgacggca tccacctgct
     6421 ctcgccgcag atcatcggcc acatcaacca tctcaaggga aaggtcctgg gcgagcgctc
     6481 ggtgagcctc aacctggacg agacgctcat cgccctgagc atcagcgccg cgaccaaccc
     6541 gacggcgcag gcggccatgg agaagctcac ggacctggcc ggctgcgacc tccacagcac
     6601 gcacatgccc acccccggcg acgaggacgg cctgcgaaag ctgggcgtca acatcacctc
     6661 cgatccgcag ttcgccagcc gcaacctctt cgttatgtga taccggacgg gcgtcgcgtc
     6721 cggatcgtcc ccggcgcggg tatagtcatt gacaaaaaat ggcgcggccc gccttgtgtc
     6781 gcgaaccgcg gaccggccat gcgccgcatc gacgcgaaca atcatcatca cggacagaac
     6841 acaggagcag taatgacgaa cgcatcagag attacgttgt acagcggcgg ccacaaaggc
     6901 gccgaggcgg aattcggccg gcaggcggaa cgatatggca tgagccaggt caccttctcg
     6961 tacgacggcc acaagatgga gcatgccgtc ggcgtgcagg cgctgggccc gaaagagctc
     7021 gccgagggcg acgtgagcat ggccatcgtc tcgcagcgca tgcggcggaa cttctcggag
     7081 gtcgacgcca tccgcaaggt cattcaatcg atctatcata tggtgaacaa ggggttccag
     7141 gtgttcgcca tcggctggat tcaggacgat aacaccgtca agggcggcac cggctgggcc
     7201 gtggagctcg cgcgcctctt caatcgcccg gtgagcgtgt acgaccagga ccggaagggc
     7261 tggttcacct ggaccggcgg agaatggcag aaggacagcc cggtcatttc ccacaagacc
     7321 ttttgcggga cgggcacgcg taatctgtcc ggtgacgccg tcgcggccat cgccgacctg
     7381 tacgcgcgtt cgttcggcgc gcgatagcgg cgtcgtacgc gcgccgcgat ctgtaacaat
     7441 cgccggcgcg cggcgactaa ctcggcacat tcgtcgcgct tgcgcgcgta cgcggatcga
     7501 aacggcggga gggaatgatg ggcaccatga tgacgtttcc cgaaaccccg ggcgcggcgg
     7561 tcgccttctt cgcgatctat gcctttttcg gatggctcgt cgaggtggcg taccgttctg
     7621 caacccagcg ccgcttcgtg aacgcgggct ttctcttcgg gccgttcgtg ccgctgtacg
     7681 gcatgggggc gctcctggtg ctggcgctgg gttcgctcat ggcggggtgg cacccggcgt
     7741 tggtgtttct ggccatcggc gtcgtgctca cggcgctgga atacctcttc ggcttcctga
     7801 gcgagcggct gttcgatctc acgctgtggg actacagcgc caaccggttt aatcttcacg
     7861 gacgggtgtg cctgctgtat tcggtcgcct ggacggccct cgccttcggc gtgagcgtat
     7921 tcgtgcatcc attcgtaacc cgcttcgtgg gatggttcga cgagcaggcg ctgcgctggt
     7981 ttgcggcggc cttcgccgtg tattacgcca tcgacatcgc cttctcggtg gccgcggtgc
     8041 tcgatttccg ccgccggctc accttcctgt tggccaattt cgtcacgctg gcgaacccgg
     8101 acgtcgagcg cttttttgac accttccgcc ggctgctgcg ggccttcccc aatctcaacc
     8161 ggcagctcac cgcgaacctc agcgagaacc tcaagcgccg gatcgacgat ctgctcgcct
     8221 cggtgggcgc gcggatacag gacgagctca aagggcgcac gcccgcctac cgggagtaca
     8281 agggcatcgt tcgggacatc ctcgatcacc cggagttcaa gcggctcaag gagttctatc
     8341 accacaattc gtcgatctac gagcacgccg tccgggtctc gtttctctcg tatcgcatct
     8401 gccggtatct caatctcgat taccactcgg cggcgcgcgg cgggctcctg cacgacttct
     8461 tcctgtacga ttggcgcaat cacgacgagc ccgacctcgc gcgcgacaag ttccacggga
     8521 tccatcatcc ccgcatcgcg ctggagaacg ccacggcgca cttcgcgatc aacgacgttg
     8581 agcgcgacat catcgtgaag cacatgtggc cgctgacgct cacgcccccg cgctatcgcg
     8641 agtcgttcgt ggtggggtgt gtcgataaat acctcgcgtc gaaggagctg ctgggggagc
     8701 tgcccttgcc gggcggaagg cgaaagcggc cggggcgggc gcggcgcgtt cggacgggcc
     8761 cggcgtaggg gggcgtttca aatgacggtc gcctcggtgc gcggcggccg ctattcctcc
     8821 ggccgtatct ccgagcccgc gaagggccgc ccggtgaccg gcgcagagag cgccgactca
     8881 tggttgtatt tcgtgtcgat ccggtaggtg tcgtaggcgc tcaccgcgaa aaagtacagc
     8941 acattgttct tcatgatggg ataggacagc aggccgcggg tgtcccgcgc gcgattctcc
     9001 tcgatcacgt gattggtgac ggtgatctcg atctcgttgg tgctcgagag cgcgttggtg
     9061 atgcgccgct cccgcgagtg ggtgatgatc ccgtcgtaac gcccgggccg cgcgccgtaa
     9121 tagatcttgt agccgcagag gtcctcctcc acgttcttct tccatttgat ggtcacgctc
     9181 ttgtcgccgg cgccggccac ggccaccatg aggggcacgc gcggcggcgc gtccacgcgg
     9241 tattcgaggg cgatgtccca gaggcgcggc gagcggcggc cgtccggcga ggggaccagg
     9301 tgcgcgcgcc actggcagta tttcccgcgc aggaattcgc tcccgacgcg catgcggtag
     9361 atgtcgcgct ggttgttcac gatgcggtac catttcggct ccgggtcggc ctggccgaag
     9421 aggtcgtcgc tcgtgcgaaa ctccatccac acgaaggtgc cggaggagag ctcctcgcgc
     9481 cacgagaaga gccgcgcctg cgttccggtg gcggggagcg tgtgcacggg gccggtgacg
     9541 acgccctcgc ggttcaccgg ctcgcggccg atggtcgtaa ggcgctgatg gcgcttggag
     9601 gcgatctcgg tttcccgctt caggtcggcg atgtggcggt gcgagatgcg gaactcgtcg
     9661 atgatgccgg agaagtcctt cccgaccacc ccggcgggga gatcgtcgca ctcgaaggcg
     9721 ggccggagca cattcacgaa gggctcgccc gattcggtcg cgtacaccgc ctcatcctct
     9781 cgcccgttga tgcgcttgga aagcttgccg gacacgcggt cgaaactcac cagcgcgtgg
     9841 tgccaggcct tgagcttcag acgccttccg cggttgaggt acacgacgcg cggccgcccg
     9901 gactggtcgc gaaacacccc gtggaagcgc gcggtgagcc tcccgttttt cagcacgatc
     9961 tcgaggccgt tcttctttcc ggaggcgtat ccgatgcgcg agaagatgac gctttcatcc
    10021 cggagggaat cggcccggaa acggaactcg aggctgaacg agcccaggtc ctcgcagttg
    10081 cccagccaga ggttgcgctc gctctggagc tccacgcggt gctgcgcctt gaagaactgc
    10141 gccccgcctg cgccgtggat gccccgcccg cgcaccggct cgtaagaggc gtatttgatc
    10201 cggtagtggc cggcgtcgtc ggtccccatc gcgacgacgg ggcggttgaa cgagagcacg
    10261 cagtcggtga tctccggcag ggcgcttgat tcataggtga tgtcggcgat gcgatagaac
    10321 cgccggccgt tcgcggccgt gtcctcgatc acgttcagga gctcgcagcc ctcggaggtg
    10381 aagacgatgc tccccgcgcg gccgtccgcg acgaggagcg cgagcgcggc cagggccgcc
    10441 gcgatgattc cccgccgccg tgtcattgcg tgcggttgtc ccgctcgata gcgtcgatct
    10501 tgtcgaggcg ccgctgatgg cgcccgccct cgaaggggct ttcgaggaag cgctgcacga
    10561 tgcgaagcgc gaggtccgat cccacgacgc gcgcgcccag caccagcacg gtcgcgttat
    10621 tgtgccgtcg cgccatctcc gccatgaact cgttggtgca cagcgccgcg cgcacgcccg
    10681 ccaccttgtt cgcggcgatg gaggccccga tgccggtccc gcagatggcg atgcccagat
    10741 cggccgaacc gtcgcgcacc gccgcggcga ccgccgcggc gtagtcgggg taatcgcagg
    10801 attcctccga gtgcgtgccc acatccgtaa aggtgacgac ggccgcgggc acgagcggcg
    10861 ggagcgtttt cacgatgagc atcttgaggg ggtagccggc atggtcggag ccgatggcaa
    10921 tcttcatcgc attctcgcgt gttcacgggc cgtacggcgt cgggcccgaa ttatgtctcg
    10981 atagattatc ctacaatgcg gcgaagcggg cgtcaataca tatctttcac ggcgcgggat
    11041 atttttatcc ggctccggca gccgttatcg aatcacatcg cacacgatga tcgagaggtc
    11101 gtcattgaag gccgccgttt cggtgtattc gtgcaggtgc gacaggagcg attcggagag
    11161 ctcgcggggc gtcgatccgg catggccgcg tatgaagtcg ccgagccgct ccatgccgaa
    11221 gagttcgccc cgcgcattgc cgcactcgat gatcccgtcg gtgaacgcga tcacccggtc
    11281 gcccggcgag agctgccgct cgaccgccgc aaaccggtac tccgggatcc aaccgagcgg
    11341 aatgccgtgg acgcccagct cggagaggac gccgctgtcc cggttgaaga cgatgacggg
    11401 ccagtgtccc gcgctgctga tccgaacgca ccggttcgcg acgtcgacga cgcagcagac
    11461 ggcggtcacg aagtttccgc ggaccgacga gcggcagacc gcccgattga gctcgccgag
    11521 ggttccggat ggcgaaccct gcgtctcgat gctgtcgtaa gtcattttgg ttatgaggga
    11581 caggaatgcc gcatggaggc cgtggccgga tgcgtccgca agcaaaatct cgagcggtcc
    11641 gccggcgtgt ttgaatccgt aaaaatcccc cccaaccttg tccatggaaa cgttgagcgc
    11701 gaaatagtcc aggccctcga tgttcgccag ggcctccggc atgagcttgt tgaggatgag
    11761 gcgggccatg tccagctcgg actcgatctg gacgttccgc tcgcgcagcg cgttgttgat
    11821 cgtgacgagc tcctgggtgc gttcgtgcac ctgcgcctcg aggagcgtgt tgttgctttc
    11881 tatgatgcgc cgctgctcgg tcgtcgtgcg gtcgtagttc tcggtcaggg ggatgatccg
    11941 gtacaccgcg aaccagacga tgatgctcac cagcatgatg aacacctcgc gcaggaccac
    12001 gaacccgctc acgccgcggc catgctccac ggactgtatg gacatggggg ttccggacag
    12061 aacggcggcc accaggaagg ccagccagcc cgcaagcgcc gccacggcga ggaccttgaa
    12121 gaggcgcgtg tcgtacagga actgattgat gacgacggcg atgatgattt gcaccgacga
    12181 attgtacgag gagagcgcga cctcccagcc gtagttcgag acgtagatga acttctgcgc
    12241 gatcggaatc atcacaaaga tcgacgcggc cgcccaggaa taccccgcgg cgggccggcc
    12301 cgcctttttt ctgcgatatg tctgcaggga gcacgtcagg gaggtcgcga tgaggacgct
    12361 tggcgtgatg agtccttgcc cgcgcacatt ggcgagcatg agcatcgcga aggccacgat
    12421 gaagaccgag aggacgagga agaggtagat gaggcttccc tgcacctcga gttcccgcca
    12481 ggcggacgat cgcggcggtt ccatcgtgtg ctcgcgtgtg attcggtgca atatcttctg
    12541 aatcatggcg catgtcgtgt ccggcggcgg caaaccggaa cgggggagac cctcgcgcgt
    12601 tctttgcgaa aagtcaaatg aaattccgcc tcccgcatgc gcggctccgg cggtttcaaa
    12661 aatcccattt ttctcttgtg ttgatacgcc gcgtctgtta cggtttcccc ggcatgaaaa
    12721 gaacgaatgc gcgaaccgta ccgggatacg tcgccggcat cgtcctcgcg gcggcgctcg
    12781 ccatgggcgt cgcggcgcat gccgccgatt tcaccggcac ctggaaaacg aaccggggga
    12841 cgctgcagct tgcgcaggag ggcaacctgg tgatcggccg gttctcgggc gaggcgcgtt
    12901 ggcgcatcga gggaacgacc agcggcaatc gtctcgaatt cgtctgggcc gatcccgcgg
    12961 ggcggcgcgg ccgcggcgtc ctggtcgcgt atcccggcta ttgctcgttc gagggggcct
    13021 ggtggcccgg gtcgctcgtc gggcatggcg gcgagtggcg cgggatcaaa acgagcgacg
    13081 actatttctg cggcggggac gagtgacatg tccgcgaacg gcacggccct ggtcatcatc
    13141 gacatgcagc ggtattacct gagcccggcg tcccccttct atcgctattt cagcgacctc
    13201 tcgccgggct gcatggacta catctcgaaa cgctgcgccg atacgacggt ccccgccatc
    13261 accatgctcc tcggcgcgtt ccgcgcgcat ggcgcgcccg tcgtctactg ccggctctgc
    13321 ggcgtcgatc ccgaacgccg cgaccttcat cgcttctttc gcgacaccta cgttcgcgcg
    13381 cgtgccgcgg gctacgacaa tgtctatccg ctggcgggcg accccctcgc cgagatcgac
    13441 cggcgcatcg cgccgcgatc gggcgaggcc gtgttcacca agaccacctt cagcgccttc
    13501 gcctccacgg acatcgacgc gcgcctgcgc gcgcggggta tcgacacgct cgtcttcgcg
    13561 ggtttggcca ccagccagtg cgttgagacc accgcgcgcg atgcgtcgga ccgcggcttt
    13621 ggcgtggtgc atgtcgccga cgcgcaggcc gactacagcg aggagacgca ctgcgcctcg
    13681 ctctacagct cgcagggtgt gtgcgggggt gatgtggtcg atgcggcgga gtatctcgca
    13741 cggcgcgggt gggaccgccc cgcggacgac cggcgcgatt agatgcgcag cttgcgggtg
    13801 atggcgtcct gcagggcttt gatttgctcc aggtgcgtgc cgatgtacag cgccgcatag
    13861 accgagatcg ccacgcccag catgatgctg aaggggacct cgaagtgccc gccgaagtgg
    13921 tccttgagcc agatgagcga atcgctgaag gggaccagta cgatgctgga gaggatcgcc
    13981 ccgcccacgg cgacccactt gccggtgccc acatttgtcg cattctcgat ccgcgagcgc
    14041 cgcgcggcgt gcatcaccgc gtcggcgagg tccgggggcg cgtcgaaggg ggcctggcgc
    14101 aacagcgcgc ggaactcccg gtccaggcgc gcgatctccg cgcgacagcg cgcgcagcgg
    14161 agggcgtgga atttgaccgc gagcgcgaca taggcgcggt tgtcggtttc gagatagcgg
    14221 cgcatcgcct tatcgcagtt catggtagtc ctccgcaatg gtcccgcgca gcgcgtctcg
    14281 aagcatctgc ttcgcgcgaa acacgttcga ttttatcgta ttgaccggta tcccggtcat
    14341 ctcgcagatg tcggcgtagg gcatgccgta aaaaaagtag cagtccaggc acaggcgaaa
    14401 attctccggc agcgcctcga cggccctctc cagcaacgcg cggatttcat tgtgctcgta
    14461 cgagcgctcc ggcgtcgcgc cccggctctc cagcgcctcc tcggcgagcg tgcccggatc
    14521 gcgcgcggcc ttcacggcgt tgatgccgtg gttgtacgcg atgcgggcga gccaggcgcg
    14581 gaagggcgcg ctgcccttga aggtgcccag cttgtcgaag gccttgatga gcacctcctg
    14641 cgcgaaatcg cgcgcatcgt catcgttccg tagaaagcgc cgtccgatgc tgtacaccat
    14701 cccctggtgc cgcgtgatga tgatgcgaaa ggcctcggtg tctccgcgta ggacccgccg
    14761 gacgacgtat tcgtcggttg cggtatcggc gttcatctcg catcgttgga cggtccgccc
    14821 cgcatcgagg tgtacagctt gaaaaatatc aggagactcg cccccaccgc gaaggggatc
    14881 atgccgctta acacgctgta ggcaaacccc tccttgatga ggaagaaggc gatgagacag
    14941 gccccgatgg ccgtgagcag aagccccgcg aacaggctga agaccggaag gtcgaacttc
    15001 ttgggcgtgt acatccgctc gcggatcatg agcatcttct ctcggtgcgc gtagagcaga
    15061 tagaagaaga tcacgaccga tcccatgacg atccccacga tggggacgat ggtgacgatg
    15121 acctgcgccg ctgccgaggg aacgttttgc atgccgccca tacgattctc gctcctattc
    15181 ctggtcgaag gtgacgcgct gacgcaggcc ctgccgcagc aggtgcgccc gcatggccca
    15241 gtgcgttatg cgctcttcgc accgcgactc ggtcgcggcg aactgcgcgt gcaatgattc
    15301 tggcagatcg cccggaagtt gtccatactt ttttcgcagt tgggccacga tcgcccgtcc
    15361 gcgccgctcg atccgccgcg ggtgcgccgg cgtcggtttc gcaagcatcc gctcgagttc
    15421 ggcgtcgagt cgcccgacgg cgccctccag gacggcggcg aagcggtcgg ccgcgaggat
    15481 cccggtggtg atctcgttga agtgatactc cttgatcgcc aggttcgccg cgagcgcgaa
    15541 gcggcgcggg ttgatccaga gcgcccgggc gaggaaggcc agatagcggt gcccatagga
    15601 cgagaaagtc tgcacgaaca gcgagcggac gagcgccctg aggtcgcgaa cggtcgtcgc
    15661 gccgctcgcg accgggcgcc gcggcagccg ccgcagcagg tcgagcccgc gtgcgaagta
    15721 gtggcgcggc gtgtagatat gctcgatgat tcgcttgtag ccggccacaa ggtcggcgtg
    15781 cgggatcgtc gtgacgaagt ttacgtcgag atcgcagttg tcgcccgtgg tctcggcgag
    15841 cagccgtccc t
//
LOCUS       DAORHF010000060        15755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2821_length_15755_cov_5.275987, whole genome shotgun sequence.
ACCESSION   DAORHF010000060 DAORHF010000000
VERSION     DAORHF010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15755
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2821_length_15755_cov_5.275987"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PKJ16_07115"
     CDS             <1..614
                     /locus_tag="PKJ16_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46795.1"
                     /translation="RMIPRVLVARSLRWDAYRSRAAADADAPVPRAALALEPLAPDYR
                     RMLAEWGELTDLPDPILPANTAHHDQSRPAPLQERMLAIHSGNAAEQEELLQFARHSC
                     LDAGRPDMPVDTLFPDRHTFPATGLMRTYRFIAAGCGYNMAAAARRALPGQEFFLHPF
                     ERRFDDQRARLRNLRTGLWRGGAENGAHAAACWVAHHALMALK"
     gene            708..1904
                     /locus_tag="PKJ16_07120"
     CDS             708..1904
                     /locus_tag="PKJ16_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HNV46796.1"
                     /translation="MKHLLAFALSIAFVAPILADGGTDVTVYTMPGCGRCSFTISELN
                     KQRVPFTERSTSDPAAKRAMWDLIGASGKHAGGGVSMPVIVVKGAVHFSIADLRAFVA
                     SIPSLLSGSSTPAKPDGGKAAAPDGFAREITDRHNHFRKMHGAPALSWSPAINAYAQE
                     WADRLAREDRMYHRSPNKYGENIYWISGGRPGGASPVNSWYAEIKDYDFRRPGFSMGT
                     GHFTQVVWAGSTELGCGTATSRRGGTYVECNYNPPGNYAGRFPANVRPAGSAPAQETA
                     ALPTFMNVWTYGNAVVNRPYPGWAQKRLPIANHYTGAENGVYVAVYSRTAAASVYPVG
                     GGIHVMGLIRLKGTWNGRIAEPDGWAGRDISAAREFKRLAGQYYQSCGGDCWVGGDTG
                     GFFGKR"
     gene            2201..2554
                     /locus_tag="PKJ16_07125"
     CDS             2201..2554
                     /locus_tag="PKJ16_07125"
                     /inference="COORDINATES: protein motif:HMM:NF039485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HNV46797.1"
                     /translation="MGLLDTITAVTNTLGKERGANQDLLNGVLGLFKGNGLKDLLDAF
                     SRNGLEDVVRSWIATGANREISVGDVERALGPEKLAQMAQQAGVPVDNVSSLISKMLP
                     DIVDKVTPKGTLDQD"
     gene            2587..3042
                     /locus_tag="PKJ16_07130"
     CDS             2587..3042
                     /locus_tag="PKJ16_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HNV46798.1"
                     /translation="MALSPLVWLAAGIVIMALEIIMPGFIIFWFGAGAVLTALFVFIG
                     IIPGDAAEIQWIFFFLSSLAMLGAWQFFFKKRFKGETADLSRDATLANLRGRVTSRIT
                     PGVPGEVELYNPYHGIKKWHAESADTIEVGEEILVQDASGIRLIVTRAG"
     gene            3071..3946
                     /locus_tag="PKJ16_07135"
     CDS             3071..3946
                     /locus_tag="PKJ16_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV46799.1"
                     /translation="MIYLAIAIGLFVVFLILKGVRIVPQAENWIVERFGKYANTLNPG
                     LNLINPIFSRVATKIDIRESVLDLPKQGTITSDNASLEVDGIVYFKIMDPYKAYYGIQ
                     NLHYAIQNLAQTNIRSILGRLTLDESLSSREKINADLLITLDEATDAWGTKITRVEIR
                     DIQPPDDIVNAMTLQMKAEREKRARILEAEAKKEAAEREAEGIKRAQILAAEARKESA
                     ERDAEARERLALAEANAIKVVTDSLKASNGDPLTYLLGQEYVKGLVKLGESANSKFVL
                     LPADLLDTVKNIFKK"
     gene            4136..4357
                     /locus_tag="PKJ16_07140"
     CDS             4136..4357
                     /locus_tag="PKJ16_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46800.1"
                     /translation="MKTTTIKSGSIKLPLEIARRLRGKDIEIIETNDGVLLRPTQGII
                     QSTRGFLKGKGSFSSSKFMSEKQKEKRFE"
     gene            4361..4774
                     /locus_tag="PKJ16_07145"
     CDS             4361..4774
                     /locus_tag="PKJ16_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV46801.1"
                     /translation="MKTYILDACALIAFILDEDGAENVEKLLSLSHERKCALFINKIN
                     LLEIYYNLRKEYDPDTFREAFSRICALPITIIDTISDEVLIEAGRLKSENRISLADSI
                     ALAEAITRNASLVTADHHEFDRLGRTEHIKFHWIR"
     gene            4844..5221
                     /locus_tag="PKJ16_07150"
     CDS             4844..5221
                     /locus_tag="PKJ16_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46802.1"
                     /translation="MRQPKKNRVLIVEDEVITSMDLQSHLQSLETVGEVVIKSTGESA
                     LEYAKEMNYDIDLLVMDVNLGKGMNGFQFAKEIHKIKYIPMMVITAYSTTGFDFRELP
                     ACGFMSKPIDTDVFIRIIKAILS"
     gene            complement(5216..7645)
                     /locus_tag="PKJ16_07155"
     CDS             complement(5216..7645)
                     /locus_tag="PKJ16_07155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46803.1"
                     /translation="MRNSHDDGRTADILDSLPEIVFECDKSGRLIFVNQRACNMLGYA
                     MDELLAISVFDLIREDDRRAAKTVMTRILDSARDYVGIETCLVAKTGSSVSVQVYTNR
                     ILDETGAVSGIRGIAVDTTGIRTAIARLAESEEKYRKLVEYANDIIYQCNYRGDFLEM
                     NQFGLRNLDYTLEELRKKNYTDLIPAEFRDREYAFYSEQLRSKSEVPTYRELPLVRKD
                     GTVLWVAQHVSLVTEKGVLSFYGFARDITEFKRVQQELAESELMFRTMIEESSDLIIV
                     VDRAGTLRYLSPSVARMLGFSANAAIGHDIFTHVHEDDVGRLRRMFEESIADPDRLFV
                     AEYRYRNAANEWRTLEMRGRNLLHKAYIGGILVNIRDITEQKALLDEIKRREELYRLI
                     TENVTDIIWVVSLETLRFMYVSPSVLRMRGVTVEEALVEPLESVFLPEEYERVMGVLA
                     AELEAERSGTRDPDRIVIIEAQQYRKDRSIFWVELRASFVYDDAGRAYAIQGSTRDIE
                     KRKEIERSLAEYREHLEELVAQRTGELNESNAELRREIVEHLRDEERLRAKNERMNRE
                     LDEARRVQQMLLPAKMLTDSVEISYRYNPLVNVGGDFLSFTLLQEHNGVGVFIGDVAG
                     HGVSAALYTFYVRSLTDRVCRRWGGSPKNYMSILSAELIKGMKGNYLTGIYGTFFPAP
                     GGGVAFTFAKGGHPYPIMHRRSSGETTLIIQRGVALGLFEGTQYDEQRIDLARGDRIY
                     LYTDGMPETFDSHRNMLDYEGFLEIVRGAERFNQPLEDTLDYIMVRINQFRGSAPIDD
                     DMLIIGVEVRS"
     gene            complement(7729..8595)
                     /locus_tag="PKJ16_07160"
     CDS             complement(7729..8595)
                     /locus_tag="PKJ16_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46804.1"
                     /translation="MLQDTTVTGRLVARDDGAPALVDDRGVDLLPDERIWGGYLAHWS
                     GRRASARRLPQRDYETGRPIVIMWPERTGERAPFAELYYNERLVKYPASRFGHVAINV
                     NGGVFNFSHLINENEEMTEEEFLYRPPLGEFAPHPVTGLFDVADPVRPYYDKFGRNFM
                     RTIHVLRIEGFDADRLAAHYRREIEVNRSNPDPARPEKYRDFSVVSRSCVTIIRDGLR
                     AAGVPHVGGHFPRDLFISAAYHLTRPQTQSGLRVRLYRMPQLVVPEAPVSAPTPIINP
                     ANRLRALLLPKY"
     gene            8836..10431
                     /locus_tag="PKJ16_07165"
     CDS             8836..10431
                     /locus_tag="PKJ16_07165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46805.1"
                     /translation="MNTGELTARQILEIKGAFVLTILIVVMASVAVMFQLLAGIALPV
                     VASGLALYLVAIALAYLIFRRKRAVRPTAALEWVMATLMFIMTNLVKYNYARNMDWTY
                     AVQSYHISAIAVAMVLILQFLYNKKLFVTFSVLTFVNWGVFLLLADMNGVEMPLGSFI
                     DGKINYGIVMLREYYFLITLSIVSFISYKNITTVDGYDAKTMKQTALIEEQASRQIAI
                     AETIAARMNALIVELERENAVIADFNEKMQSQASTFEEISATLEELQSSAETIAVSAT
                     RQTDDNTTLEGRITDFRSVKKETGERLDRALSAITAVVSTTSTGQEKLEMVERTISNI
                     NEQSAAISDTTSIITDIADKINLLSLNASIEAARAGEYGRGFAVVADEIGKLAYQTAE
                     SIKEIERILSMNAKATGEGVAVVQDAAVIIKGMIGNMEDSTGTIQSLRQSIDDEERHI
                     DAIIEQLTKNIDASRQIGQGTQEQRRAIESSATAIEYANEVIAKMAGDVAEIARASDT
                     IFRNARDLLDEAQKSLDRERAAV"
     gene            10441..11013
                     /locus_tag="PKJ16_07170"
     CDS             10441..11013
                     /locus_tag="PKJ16_07170"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HNV46806.1"
                     /translation="MRISVDHIFWIVLATLIGAAAFFTSGVVGFKRSMTGADIQFASG
                     QKVAVTRIIDGDDLAVRLGDHQLRIRILGIATFDPNLNDPLVRGAAGATTAYLSRTLL
                     NNEVELVYDSFARDKNGRVLAYIHRNALDIGEDMVANGLALTFTRYPFSRMKRYLAAE
                     RLARDERTGLWGDPALARRARDLQKVWERQ"
     gene            11023..12081
                     /locus_tag="PKJ16_07175"
     CDS             11023..12081
                     /locus_tag="PKJ16_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNV46807.1"
                     /translation="MHFFIMFIRKTALSALGNDAFKAIVLFAALLWYATAGFLYFELP
                     QKPDLGWGDALWWTLVTMATVGYGDLFPTTAGGRYLVGLPTMVFGIGFLGFIITSVAS
                     QLIETRSRRLQGMLQTRLADHIIIVNFTQQEEILALVRELRSDESTGKKEICLIDERL
                     PQIPPQFVDLGVSFVKGNPTDEDVLRRACLDTASHAIILSRDRHDPHADDQNLVTVMV
                     IEKIHPAVFTVVEVIDPRKTHQFELAGADSAVCVNEFAVNLIIQELQDPGVKAVVDDL
                     TSNTEGEQLYIVPIERMKDWRYRELAAWGQERRTLVIGLMTDGRPRLNCAPDELVRDT
                     DRAIVIASVRPQNIRTAG"
     gene            12091..12945
                     /locus_tag="PKJ16_07180"
     CDS             12091..12945
                     /locus_tag="PKJ16_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46808.1"
                     /translation="MRRPDHDIDLFDARMMGLDGLCAVYVLRGSRNVLIDAGTRDCAP
                     ALIDFLRDARIVPHIVVVTHNHHDHIGAIAPLRKAFGPIEVLCGPGGRERLRDPNAIN
                     RLFSPIAFEPIEGVTELADGETVDCGSRRLRALHTPGHSDDSISVIDLDTKTLFPGDL
                     PGDWLWGHTFLSAHITPDFSEEKYFASTERLAALDIDSSALAHYGFFDGPHARSILQE
                     QKTRYLEWRDALVPAYRETGNTHALVPILKRFLVGSRFESVPGYDAVIEALAGWCVMG
                     YLSAGIIE"
     gene            12949..>15755
                     /locus_tag="PKJ16_07185"
     CDS             12949..>15755
                     /locus_tag="PKJ16_07185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46809.1"
                     /translation="MSANRPSYEELQQRVRELEKRVVELEATTGTADGTLSDQSTMYK
                     TIFHLSPVAIGLTRFDDGTIYQGNDAYFEEIGYSREEAIGRTTVELGFWANPGDRDRI
                     KAILLDKGSYHKMELPFRKKDGTIYHVMQSAELVTIGDRMYIIGVHINITHLKQLERD
                     LLEKTEEMEATIEELNQSNDELNATVEELEATNEALIATQEELRTSHNEISAREERFR
                     FLTERLNDIVFILNTNGEITYSSPNVVSILGYSVDDVSTAPFRDFVHPDDYPIAAESF
                     ARHMRGETHVSEFRVRKKSGEYCWVRDSARPIVVNGAIAYIQGTLSDITDRKNAETAL
                     MESEFKYRMIFEHSPLGILHFDVDGAITACNDTFVSVIGSSREKLIGLNMLNLPDTRV
                     AAAVREAIAGGRGQFEGEYRSVTADKVTPVRTLFEAIVGPDSAFLGGVGIIEDVSERA
                     TALAALRESEERFRSLVEAAPYGIVVHIDGRAVYANTAAITMIGFESLEDIVGGSIIE
                     QVHPDDRAMVVERLQQQLKPGEALPPIEERLITKSGDIIVCEVDSILADYQGRTAVFS
                     FVNNITDRKAIESALMAERERLAVTLRSIGDGVIATDAAGRVTLLNKVAEDFTGWTAA
                     EAMGRHIAEVFVIVNEFSREPAGNPIDRVLASDAIVGLANHTMLLSRDGRERIIADSG
                     APIRDRSSRIVGAVLVFRDITDKQRMEMELHRNQRLESIGVLAGGIAHDFNNILTAIV
                     GNITLARLHETDRSPSATVLAEAEKAALRAKDLTLQLLTFSKGGHPIKKVVALAGIIR
                     DAASFVLRGSKVRCEFELADDLWAVEADEGQIGQVIQNLVINAMQSMPDGGIVRIVAR
                     NESASSESVTATATGPYVRIDIIDRGGGIPPEVLPRVFDPYFSTKETGSGLGLSVVHS
                     IVTKHDGLVGVTSQIGSGSTFS"
BASE COUNT         3133 a         5119 c         4743 g         2760 t
ORIGIN      
        1 ggcgcatgat cccgcgcgtg ctcgtggcgc gctccctgcg ctgggacgcc tatcgatcgc
       61 gcgccgccgc ggacgcggac gcacccgttc cccgcgcggc gctcgccctc gagccgctcg
      121 cgccggacta ccggcgcatg ctcgcggaat ggggagagct aaccgacctg cccgacccca
      181 tccttccggc caataccgcc caccatgacc agtcgcgacc cgcaccgctg caggagcgca
      241 tgctcgcaat ccacagcgga aacgccgcgg agcaggagga gctgttgcaa ttcgcgcggc
      301 actcatgcct ggatgcgggc cgtcccgaca tgccggtcga tacgcttttt ccggaccggc
      361 acacctttcc ggcgaccggg ctcatgcgca cgtatcgctt catcgccgcc ggttgcggct
      421 acaacatggc cgccgcggca cgtcgcgccc tgcccggcca ggagtttttc ctgcatccct
      481 tcgagcgccg gttcgacgac cagcgcgcgc ggctgcgcaa tctccgcact ggcctctggc
      541 gcggcggcgc ggaaaacggg gcccatgccg ccgcatgctg ggtggcgcat cacgcgctga
      601 tggcgctcaa ataacggtcc tcgggccgga ccgttttttt gcgtgacagg ccgcgcggat
      661 cgtggacaga gtgaccggcg caatcaaccc acgaacggag taaccccatg aagcatcttc
      721 tggcattcgc gctgtccatc gccttcgtcg cgccgatcct cgctgacggc gggaccgacg
      781 taaccgttta caccatgccc ggctgcgggc ggtgctcctt cacgatatcg gagctcaaca
      841 agcagcgcgt cccgttcacc gaacgatcca cgtccgaccc ggccgccaag cgcgcgatgt
      901 gggacctgat tggcgccagc ggcaagcatg cgggcggcgg ggtgagcatg cccgtgattg
      961 tcgtgaaggg cgcggtccac ttcagcatcg ccgacctgcg cgccttcgtg gcgtcgatcc
     1021 cgtcgctcct gtccggatcg tccacgccgg cgaaacccga cggcggcaaa gccgccgcgc
     1081 ccgacggctt tgcccgggag atcaccgacc ggcacaatca cttccggaag atgcacggcg
     1141 ccccggcgct ctcctggagt cccgcaatca acgcctacgc gcaggaatgg gcggaccggc
     1201 tcgcccgtga ggaccggatg taccaccgga gccccaacaa atacggcgag aacatctatt
     1261 ggatctccgg cggccggccg ggcggcgcgt cgccggtcaa cagctggtac gcggagatca
     1321 aggactacga tttccgcagg cccggcttct cgatgggaac gggtcacttc acgcaggtgg
     1381 tgtgggccgg ctccaccgag ctgggctgcg gcacggccac ctcgcgccgt ggcggaacgt
     1441 acgttgagtg caactacaac ccgccgggca actacgccgg acgctttccc gccaacgtcc
     1501 gacccgcggg cagcgcgccc gcgcaggaaa ccgccgcgct cccgaccttc atgaacgtct
     1561 ggacatatgg gaacgcggtc gtcaatcgcc cctatcccgg ctgggcgcaa aagaggctgc
     1621 ccatcgccaa tcactacacc ggcgcggaaa acggcgtcta cgtcgcggtg tactcgcgca
     1681 ctgcggctgc gagcgtatac ccggtgggcg gcggcatcca cgtgatgggt ctcatccggc
     1741 tcaaggggac ctggaacgga cggatcgccg agcccgacgg ctgggccggc cgcgacatca
     1801 gcgccgcgcg cgagttcaag cggctcgccg gccagtatta tcagtcctgc ggcggcgact
     1861 gctgggtggg cggcgacacc ggcggctttt tcgggaagcg atgagcgatc ggcgcatccg
     1921 gtgcggcttt cacgcatgcc gcatcattcg cgcgttccaa cgcttcgctc cacgacgcgc
     1981 atttatgtct tgacaggacg atgaagcgtt gctacggttt ttccgtcgtg catggttcgc
     2041 ccgggcatga gtcggcaacc gccccccaac aaacatacaa tcagtgggat acggcgaata
     2101 cgtcgattat caagactctc aaatacggaa gccgtctcgc acagaccgga acgaatgcac
     2161 atgcgattgg atccaaatcc tacgaaccag gaggaagaac atgggattac tcgatacgat
     2221 aaccgccgtc accaacaccc tcggcaagga acgcggcgcg aaccaggacc tcctgaacgg
     2281 cgtcctggga ctcttcaagg gcaacggcct caaggacctg ctggacgcct tctcccggaa
     2341 cggtctggag gacgttgtgc gatcctggat cgcgaccggc gcgaaccggg agatctccgt
     2401 cggcgacgtg gaacgcgcgc tgggacccga gaagctggcg cagatggcgc agcaggcggg
     2461 cgtcccggtc gacaatgtct cgtcgctcat ctcgaagatg cttccggaca tcgtggacaa
     2521 ggtcacgccg aagggcacgc tcgaccagga ctgatccgcg cacacgactc ataaaggagg
     2581 acacccgtgg ccctgtcacc gctcgtctgg cttgcagccg gcatcgttat catggcgctc
     2641 gagatcatca tgccgggctt catcatcttc tggttcggcg cgggcgcagt cctcaccgcg
     2701 ctgttcgtgt tcatcgggat catcccgggc gacgccgcgg agatccaatg gattttcttc
     2761 ttcctttcct cgctggcgat gcttggcgcc tggcagttct tcttcaagaa gcgcttcaag
     2821 ggcgaaaccg ccgacctctc gcgggacgcg acgctggcca acctgcgcgg gcgcgtcacg
     2881 tcgcggatca cccccggcgt ccccggcgag gtggagctgt acaatcccta tcacggcata
     2941 aaaaaatggc acgccgagtc cgccgacacc atcgaggtcg gcgaggagat cctggtgcag
     3001 gacgcgagcg gcatcaggct catcgtgacg cgcgcgggct agctcgcgga tcaaaccata
     3061 ggagggccgt atgatttatc tcgccatagc catcgggcta ttcgtcgtgt ttctcatcct
     3121 caagggcgtc cgcatcgtgc cccaggcgga aaactggatc gtggagcgct tcgggaagta
     3181 cgcgaacacg ctcaatccgg ggctcaacct catcaacccc atcttctcgc gggtggccac
     3241 gaagatcgac atccgcgaaa gcgtgctgga cctgcccaag caggggacca tcaccagcga
     3301 caacgcctcg ctcgaggtgg acggcatcgt gtacttcaag atcatggatc cgtacaaggc
     3361 ctactacggc attcagaacc tgcactatgc gatccagaac ctcgcgcaga ccaacatccg
     3421 ttccatcctg ggccggctca cgctggacga gtcgctctcg tcgcgcgaga agatcaatgc
     3481 cgacctcctc atcacgctgg acgaggccac ggacgcctgg ggcaccaaga tcacccgcgt
     3541 ggagatccgc gacatccagc cgccggacga catcgtaaac gccatgacgc tgcagatgaa
     3601 ggcggagcgc gagaagcggg cgcgcatcct cgaggccgag gccaagaagg aagcggccga
     3661 acgcgaggcc gaagggatca agcgcgcgca gatcctcgcc gccgaggcgc ggaaggaatc
     3721 ggccgaacgt gacgccgagg cgcgggagcg actggcgctg gccgaggcga acgcgatcaa
     3781 ggtggtgacc gactcgctca aggcgagtaa cggcgatccg ctcacctatc tcctgggaca
     3841 ggaatacgtg aagggcctgg tgaagctggg cgaatccgcg aacagcaagt tcgtgctgct
     3901 gcccgcggac cttctggata cggtgaagaa tatcttcaaa aaataacaaa tcccatgacg
     3961 gttcacgcaa acccctttcc gcacggagag gggtttgtca ttttcccgga cggatctatt
     4021 tccgtcttcc atggcggaaa atgtttcttc ccatgccgcc taaaataata cttgatcata
     4081 tagcgtagac aagcaatatt tgcgggaata ttcgggcccc atggagcgtg acgcaatgaa
     4141 aacgacaaca attaaatcag gcagcatcaa gctgcctctt gagatcgcac ggaggctgcg
     4201 cggaaaggat attgaaatta ttgaaacgaa cgacggagtt cttttacgac cgacacaagg
     4261 gattattcag tcgacacgcg ggttcctcaa gggtaagggc tcattttctt catcgaaatt
     4321 catgtctgaa aagcaaaagg agaagcgatt cgagtagttc atgaaaacat acatcctcga
     4381 cgcctgcgca ctcatcgcgt tcattcttga tgaagatggg gctgaaaacg tagaaaagct
     4441 cctctccctt tcgcacgagc gaaagtgcgc gcttttcatc aacaagatta atcttcttga
     4501 aatatactac aacctccgca aagaatacga ccctgacaca ttccgggaag cattcagtag
     4561 aatttgcgcc ctaccaatca cgatcattga caccatcagc gacgaggtgt tgattgaggc
     4621 gggtcgcctt aaatcggaaa accgtatctc actcgcagat tcaattgcgc ttgccgaagc
     4681 gataactaga aacgccagtc ttgttaccgc cgaccatcat gaattcgaca gactcggacg
     4741 cacggaacac attaaattcc attggatacg atagtttatt gcaaaaaaca cttgcttttt
     4801 cataccgcgc gcggtatgca tcgtggcacc gagaggaaac cccatgagac agccaaagaa
     4861 aaatcgcgtc ctgatcgtgg aggacgaggt gataacctcg atggacctgc aatcacatct
     4921 gcaatccctt gaaacggtcg gcgaggtggt catcaagagc accggcgaat cggcgctcga
     4981 gtacgcgaaa gagatgaatt acgacatcga cctgctggtg atggacgtca acctgggcaa
     5041 ggggatgaac ggcttccagt tcgccaagga gatccacaag atcaagtaca tcccgatgat
     5101 ggtcatcacc gcctacagca ccaccggctt cgacttccgc gagctccccg cctgcgggtt
     5161 catgagcaag cccatcgaca ccgacgtctt catacggatc atcaaggcga tcctctcatg
     5221 accgcacctc gaccccgatg atgagcatgt cgtcgtcgat gggggcggac ccgcgaaact
     5281 ggttgatgcg caccatgata tagtccagcg tatcctccag cggctggttg aagcgttccg
     5341 ccccgcgcac aatctcgaga aacccctcgt agtccagcat gttccgatgt gaatcgaacg
     5401 tctcgggcat gccgtcggta tacagataga tccggtcgcc ccgcgcgaga tcgatgcgct
     5461 gttcatcgta ctgcgtgccc tcgaacaggc ccagggcgac gccccgttgg atgatcagcg
     5521 tcgtttcgcc gctcgaccgc cgatgcatga tcggataggg atgtccccct ttcgcgaagg
     5581 tgaacgcgac ccctcccccc ggagccggga agaaagtccc atagatcccg gtgagatagt
     5641 tccccttcat ccccttgatg agctcggcgc tcaggatgct catgtagttc ttcggcgatc
     5701 cgccccatcg ccggcagaca cggtccgtca gcgagcgcac gtaaaaggtg tacagcgcgg
     5761 cggacacgcc gtgacccgca acgtcgccga tgaagacgcc caccccattg tgctcctgca
     5821 gcaacgtgaa cgagaggaaa tcgcccccga cgttcacgag cggattgtac cggtagctga
     5881 tctcgacgct gtcggtgagc atcttcgcgg ggaggagcat ttgctgcacg cgccgcgcct
     5941 cgtcgagttc gcggttcatg cgctcgttct ttgcgcgcaa ccgctcctcg tcgcgcaggt
     6001 gttcgacgat ctcgcggcgc agctcggcat tgctctcgtt gagctcccca gtgcgctggg
     6061 ccacgagctc ctcgaggtgc tcgcggtatt cggcaagcga ccgctcgatt tcctttcttt
     6121 tctcgatgtc ccgggtcgat ccctgaatgg cgtaggcccg tccggcatcg tcgtatacga
     6181 aactggccct gagctccacc cagaagatgc tgcggtcctt ccgatattgc tgcgcctcga
     6241 tgatgacgat cctgtcggga tcgcgcgtcc cgctccgctc cgcctcaagc tccgcggcga
     6301 gcacgcccat cacgcgctcg tattcttccg ggagaaacac ggattccagc ggttcgacca
     6361 aggcctcctc gacggttacc ccccgcatcc ggagcaccga cgggctcaca tacatgaacc
     6421 gcagggtttc cagggagacc acccagatga tgtccgtcac gttctcggtg atgagccggt
     6481 acagctcctc ccgccgcttg atctcgtcaa ggagcgcctt ctgctcggtg atgtcgcgga
     6541 tgttcaccag aatgccgccg atgtaggcct tgtggagcag gttgcgaccg cgcatctcga
     6601 gggtgcgcca ctcgttcgcg gcgttgcggt atcggtactc cgcgacaaag agccggtcgg
     6661 gatcggcaat agactcctcg aacatccggc gcaggcgtcc gacatcgtcc tcgtggacat
     6721 gcgtgaagat gtcgtgaccg atcgccgcat tcgcggaaaa gccgagcatg cgcgccaccg
     6781 acgggctcag gtagcggagc gtacccgcgc ggtcaacgac gatgatcagg tcggagctct
     6841 cctcgatcat ggtgcgaaac atgagctccg attccgcaag ctcctgctgg acgcgcttga
     6901 actcggtgat gtcccgggcg aacccataga agctgagcac gcccttttcc gtgaccaggc
     6961 tcacgtgctg ggcgacccac aggacggtgc cgtccttgcg caccagcgga agctcgcggt
     7021 acgtggggac ctcgcttttc gagcgcagct gttcgctata gaaggcatac tcccggtcgc
     7081 ggaactccgc cgggatgagg tcggtgtaat ttttcttgcg gagctcctcg agcgtatagt
     7141 cgagattgcg caggccgaac tggttcatct cgaggaagtc cccgcggtaa ttgcactgat
     7201 agatgatgtc gttcgcgtac tcgaccagct tgcggtattt ttcttcgctc tcggcgagcc
     7261 gcgcgatcgc cgtgcggatc ccggtcgtgt cgaccgcgat gccgcggatt ccgctcaccg
     7321 cgccggtctc gtcgaggatg cggttcgtgt acacctgcac ggacacggac gatcccgtct
     7381 tcgccacgag gcacgtctcg atgccaacat aatccctggc ggagtcgagg atccgggtca
     7441 tgacggtttt cgccgctcgg cggtcatcct cccggatgag gtcgaagacc gaaatggcca
     7501 gcagctcgtc catcgcgtat ccgagcatgt tgcaggcccg ctggttgacg aagatcagcc
     7561 gcccgctctt gtcgcactcg aagacgatct ccggcaggga gtcgaggatg tccgccgtgc
     7621 gcccatcgtc atgtgagttc cgcatttcgc atcgtgcccc cggccagcag tattgtcgac
     7681 atggccggac aaggcaagtc aatttcgcac ccgcgccggg cggccgtctc agtatttcgg
     7741 aaggagaagc gcgcgcaagc ggttcgccgg attgatgatc ggagtgggag cgctcacggg
     7801 ggcctcgggc accacgagct gcggcatgcg atagagccgc acgcggagtc cggattgcgt
     7861 ctgcgggcgc gtcagatggt acgcggcgct gatgaacagg tcgcgcggaa agtgaccgcc
     7921 gacatgggga acgccggcgg cgcgcaatcc gtcgcgtatg atggtcacgc aactccggct
     7981 caccacgctg aaatcgcggt atttctccgg ccgcgccgga tcggggttgc tccggttcac
     8041 ctcgatctcg cgccgatagt gcgccgcgag gcggtcggca tcgaagcctt cgatgcgcag
     8101 cacgtggatg gtccgcatga agttacgccc gaacttgtcg tagtacggcc gcacgggatc
     8161 ggccacatcg aacaggccgg tgaccggatg gggcgcgaac tcccccagcg ggggacggta
     8221 caggaactcc tcctcggtca tttcctcgtt ttcgttgata agatgcgaga aattgaacac
     8281 gccgccgttc acgttgatgg cgacatgacc gaaccgcgag gccgggtatt tcaccaatcg
     8341 ctcgttatag tagagctccg cgaagggcgc gcgctcgccc gtccgctccg gccacatgat
     8401 cacgatgggc cgccccgtct cgtagtcgcg ctggggaagc cggcgcgcgc tcgcccgtcg
     8461 gcccgaccag tgcgcgagat acccgcccca gatgcgctcg tccggcagaa gatcgacgcc
     8521 gcgatcgtcg acgagcgcgg gagcgccgtc atcgcgcgcg acaagccgtc ccgtcaccgt
     8581 agtgtcctgc agcatcatca acccccgaac cgaattttcc cgctatgatg cgcgcggcgc
     8641 aatgaatgcc gcaagctttt ttacccgaaa aaaatccgca gcgcggtaaa accgattgac
     8701 tcggtcgtga aaaagaatca tatttgattc ggactatttc cggaaggaat gcatcacagg
     8761 acgcggggcc cgaccgccac acataccaac ggtacgcccg ctccgctcgc acgataccgc
     8821 atacgaggtg agcgcatgaa caccggagaa ttgaccgccc gccagatcct cgaaatcaag
     8881 ggcgcattcg ttctgaccat tcttatcgtc gtcatggcgt cggtcgccgt gatgttccag
     8941 ctgctggcgg gaatcgcgct gcccgtcgtc gccagcggac tggcgctgta tctggtggca
     9001 atcgcgctcg cctatcttat tttccgacgc aagcgcgccg tgcggccaac cgcggccctg
     9061 gaatgggtca tggcgacgct catgttcatc atgaccaacc tggtcaagta taattacgcc
     9121 cgcaatatgg actggaccta tgcggttcag tcgtatcata tttcggccat cgctgtggcc
     9181 atggtgctca tcctgcagtt cctgtataac aagaagctct ttgtcacctt ctcggtcctc
     9241 accttcgtga actggggagt gttccttctg ctggccgaca tgaacggcgt ggagatgccc
     9301 ctcgggtcgt ttatcgacgg taagatcaac tacggcatcg tgatgctgcg cgagtattat
     9361 tttctcatca ccctttccat cgtgagcttc atctcgtaca agaacatcac caccgtcgac
     9421 gggtacgacg cgaagacgat gaagcagacg gcgctcatcg aagagcaggc gagccggcag
     9481 atcgccatcg cggagacgat cgccgcgcgc atgaacgccc tcatcgtcga gctcgagcgg
     9541 gagaacgccg tgatcgccga cttcaacgaa aagatgcaga gccaggcgtc caccttcgag
     9601 gagatctcgg cgacgctcga ggagttgcag tcgtcggccg agacgatcgc ggtgagcgcg
     9661 acgcgccaga ccgacgacaa tacgaccctc gagggccgca tcacggactt ccggagcgtt
     9721 aagaaggaga ccggcgagcg cctcgaccgc gccctgagcg ccatcaccgc ggtggtgagc
     9781 accaccagca ccgggcagga aaagttggag atggtggagc gcaccatctc caacatcaac
     9841 gaacagagcg ccgcgatctc cgacaccacc tccatcatca ccgacatcgc cgacaagatc
     9901 aacctgctct cgctcaacgc ctccatcgag gcggcgcgcg ccggcgaata cggccgcggg
     9961 ttcgcggtgg tggccgacga gatcggcaag ctcgcctacc agaccgccga gtccatcaag
    10021 gagatcgagc gcatcctctc catgaacgcg aaggcgaccg gcgagggcgt cgcggtggtg
    10081 caggacgcgg ccgtgatcat caagggaatg atcggcaaca tggaggactc cacgggcacc
    10141 atccaatcgc ttcgccagag catcgacgac gaggagcggc acatcgacgc gatcatcgag
    10201 caactgacga aaaacatcga tgcgtcccgg cagatcggcc aggggacgca ggagcagcgg
    10261 cgcgcgatcg agtcgtcggc cacggccatc gaatacgcaa acgaggtgat cgcgaagatg
    10321 gccggcgacg tggccgagat cgcgcgcgcc tccgacacca tcttccgcaa tgcgcgcgac
    10381 ctcctcgacg aggcccaaaa gagccttgac cgtgagcggg cggccgtcta ggatgcgtcc
    10441 atgcgcatct ccgtggacca catcttctgg atcgtgctcg cgacgctcat cggggcggcc
    10501 gcgttcttca ccagcggcgt cgtcggcttc aagcgttcaa tgacgggggc cgacatccag
    10561 ttcgcatcgg ggcagaaggt cgcggtcaca aggatcatcg acggagacga cctcgcggta
    10621 cgcctgggcg accaccagct ccggatacgc atcctgggca tcgccacctt cgatccgaac
    10681 ctgaacgacc cgctcgttcg cggcgcggcc ggcgccacga cggcctacct gtcgcggacc
    10741 ctgctcaaca acgaggtcga gctcgtctac gactcgttcg cacgggacaa gaacggccgc
    10801 gtgctcgcgt acatccatcg gaacgccctc gatatcggcg aggacatggt cgcgaacggc
    10861 ctcgcgctca ccttcacgag ataccccttc tcgcgcatga agcggtatct ggccgccgaa
    10921 cgcctcgcgc gcgacgagcg caccggcctc tggggcgatc ccgcgcttgc gcgacgggcg
    10981 cgcgatctgc aaaaagtgtg ggagcgccag tgagataacg ccatgcactt cttcatcatg
    11041 ttcatccgca aaacggcgct ttcagcgctc gggaacgacg cgttcaaggc gatcgtcctg
    11101 ttcgcggccc tgctctggta cgccaccgcc ggctttctgt atttcgaact gccgcagaag
    11161 cccgacctcg gctggggcga cgccctctgg tggacgctgg tgaccatggc gaccgtgggc
    11221 tacggcgacc tcttccccac gacggcgggc gggcgatact tggtcggcct gcccaccatg
    11281 gtcttcggaa tcggctttct tggatttatc ataacgtcgg tcgcctcgca gctcatcgaa
    11341 acgcgatcac ggaggttaca gggcatgctg cagacacgtc tcgccgacca tatcatcatc
    11401 gtcaacttca cgcagcagga ggagatcctc gcgctggtcc gggagctgcg ctccgacgag
    11461 tccaccggga aaaaggagat ctgcctcatc gacgaacgac tgccccagat cccgccgcag
    11521 ttcgtggacc tcggcgtctc gttcgtcaag ggcaatccca ccgacgagga cgtgctgcgc
    11581 cgcgcctgcc tcgacaccgc atcgcacgcc atcatactct cgcgcgaccg ccacgacccc
    11641 cacgccgacg accagaacct cgtgacggtc atggtgatcg agaagatcca tcccgcggtg
    11701 ttcacggtgg tcgaggtgat cgatccgcgg aagacccacc agttcgagct cgccggagcc
    11761 gacagcgccg tttgcgtgaa cgaattcgcc gtgaatctca tcatccagga actgcaggac
    11821 cccggcgtca aggcggtcgt ggacgatctg accagcaata ccgaggggga gcagctctac
    11881 atcgtcccca tcgagcgcat gaaggactgg cgctaccgcg agctggccgc ctggggacag
    11941 gagcgacgga ccctggtgat cgggctgatg accgacggtc ggccccgact caactgcgcg
    12001 cccgacgagt tggtgcgcga caccgaccgc gccatcgtga tcgcgtcggt tcgcccacag
    12061 aacatcagga cggcgggctg acggcggtcg atgcgacgcc cggaccacga catcgacctc
    12121 ttcgacgccc gcatgatggg gctcgacggc ctctgtgcgg tgtacgtgct tcgcggttcg
    12181 cgcaacgtgc tcatcgacgc cgggacccgc gactgcgcgc cggccctcat cgacttcctc
    12241 cgggacgcaa ggatcgttcc gcacatcgtc gtcgtcacgc acaaccacca cgaccacatc
    12301 ggcgcgatcg ccccgctgcg caaggccttc gggcccatcg aggtgctgtg cggccccggc
    12361 gggcgcgagc gactgcgcga ccccaacgcc atcaaccgac tgttctcgcc gatcgcgttc
    12421 gagcccatcg agggcgtgac cgagctcgcc gacggcgaga ccgtcgactg cggctcacga
    12481 cgcctgcggg ccttgcacac gcccgggcac agcgacgact ccatctccgt gatcgacctc
    12541 gacacgaaga cgctctttcc cggggacctc cccggcgact ggctgtgggg ccacacgttt
    12601 ctcagcgcgc acatcacgcc cgatttctcc gaggagaagt acttcgcgtc cacagaacgc
    12661 ctggccgctc tcgacatcga ttcgagcgcg cttgcgcact acggcttctt tgacggacct
    12721 cacgcgcgct cgatcctcca agagcaaaag acgcgatacc tcgagtggcg cgacgcgctc
    12781 gttccggcct accgcgagac gggtaatacg catgcgctcg tgccgatcct gaagcgattc
    12841 ctcgtcggct cgcgcttcga gtccgtgccc ggctacgatg ccgtcatcga ggcgctcgcc
    12901 ggctggtgcg tgatgggata tctgagcgcg gggatcatcg agtaagccat gtccgcgaat
    12961 cgtccatcat acgaagaact gcaacaacgg gtccgcgagc tcgagaagcg cgtagtggag
    13021 cttgaggcga ctacgggtac ggccgacggg acgctttcgg accagtcgac catgtacaag
    13081 acgatcttcc acctctcgcc ggtcgcgatc gggctcaccc gcttcgatga cggcaccatc
    13141 taccagggga acgacgcgta cttcgaggag atcggctatt cgcgcgagga ggcgatcgga
    13201 aggaccacgg tggagctggg cttctgggcg aaccccgggg accgcgaccg catcaaggcg
    13261 atccttctgg acaaggggtc ctatcataaa atggagcttc ccttccgaaa gaaggacggc
    13321 acgatatacc acgtcatgca atcggcggag ctcgtcacca tcggcgatcg catgtacatc
    13381 atcggcgtcc acatcaacat cacccacctc aaacagctcg agagggacct cctcgagaaa
    13441 accgaggaaa tggaagcgac catcgaggag ctcaaccaat ccaacgacga gctcaacgcg
    13501 accgtcgagg agctcgaggc cacgaacgag gcgctcattg ccacgcagga agagctcagg
    13561 acgagccata atgaaatttc agcacgggaa gagcgtttcc gcttccttac cgaacgcttg
    13621 aacgatatcg tttttatcct taacacaaac ggggagatca cctacagcag cccgaacgtc
    13681 gtttcgattc ttggctactc agtggatgac gtgagcacgg cgcccttccg ggactttgta
    13741 catcctgatg attatccgat cgcggcggag tcgttcgcgc ggcacatgcg tggcgaaaca
    13801 catgtgagcg agttccgcgt cagaaaaaaa tcgggggagt actgctgggt acgcgattcc
    13861 gcgcgtccaa ttgtcgtcaa tggggcaatt gcgtacatcc aggggactct ttcggacatc
    13921 accgaccgaa aaaatgcgga aacggcgctc atggaaagcg aattcaagta ccggatgatc
    13981 ttcgagcact ctccgctcgg cattctccac tttgatgtgg acggcgccat caccgcctgc
    14041 aacgacacgt tcgtttccgt cataggatcg tcccgcgaga agctgattgg cctcaacatg
    14101 ctcaatcttc ccgacacccg ggtggcggcc gccgttcgcg aggcgatcgc cgggggccgc
    14161 ggccagttcg agggcgagta ccgctcggtc accgccgaca aggtgacccc ggtgcggacg
    14221 ctcttcgagg cgatagtcgg tcccgacagc gccttcctcg gcggcgtggg catcatcgag
    14281 gacgtgagcg agcgcgccac ggccctcgca gcccttcggg aaagcgagga gcgctttcgg
    14341 agcctggtgg aagcggcgcc gtacgggatc gtggtgcata tcgacggacg cgccgtatac
    14401 gcgaacacgg ccgcgattac catgatcggc ttcgaatcgc tcgaggacat cgtgggcgga
    14461 tccatcatcg agcaggtcca ccccgacgac cgcgcgatgg tggtcgagcg actccagcaa
    14521 cagctgaaac ccggcgaggc gcttcccccc atcgaggagc ggctcatcac gaaatcgggc
    14581 gacatcatcg tctgcgaggt ggattcgatc ctcgccgact accagggccg cacggccgtc
    14641 ttcagcttcg tgaacaacat caccgatcgc aaggcgatcg agagcgcact catggccgag
    14701 cgcgagcggc tcgcggtgac ccttcgctcc atcggcgacg gtgtgatcgc gaccgacgcc
    14761 gccgggcgcg tgacgctcct caacaaagtc gccgaagatt ttaccggctg gaccgccgcc
    14821 gaggcgatgg gacgccacat cgccgaggtg ttcgtgatcg tcaacgagtt ctcgcgcgag
    14881 cccgcgggaa acccgatcga ccgcgtgctc gccagcgacg ccatcgtggg attggccaat
    14941 cacaccatgc tcctctcccg cgacggccgc gagcggatca tcgccgacag cggcgcgccc
    15001 atccgggacc gctcgagccg catcgtgggc gcggtgctgg tgttccgcga catcaccgac
    15061 aagcagcgga tggagatgga gctccaccgg aaccagcgcc tggaatcgat cggcgtgctc
    15121 gccggcggca tcgcgcacga cttcaacaac atcctcaccg cgatcgtggg aaacatcacc
    15181 ctggcgcgcc tgcacgagac ggaccgttcg cccagcgcga ccgtcctcgc cgaggccgag
    15241 aaggctgcgt tgcgcgcgaa ggacctcacc ctgcagctcc tcacgttctc gaagggcggg
    15301 cacccgatca agaaggtcgt cgcgctcgcc ggcataatcc gcgatgcggc aagcttcgtg
    15361 ctgcgcggca gcaaggtccg ctgcgaattc gaacttgccg acgacctatg ggccgtagag
    15421 gcggacgagg gccagatcgg ccaggtgatc cagaacctgg tcattaacgc catgcaatcc
    15481 atgcccgacg gcggcatcgt tcgcatcgtc gcccgcaacg agagcgcctc gagtgaatcc
    15541 gtcaccgcga ccgcgacggg tccctatgta cgcatcgaca tcatcgaccg gggcggcggc
    15601 attccgccgg aggtcctgcc gcgcgtgttc gatccgtact tctccacgaa ggaaaccggc
    15661 agcggcctcg gcctctcggt ggtccattca atcgtcacga aacatgacgg cctcgtcggc
    15721 gtcacctcgc agatcggcag cggttcgacc ttttc
//
LOCUS       DAORHF010000061        15715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2829_length_15715_cov_5.246041, whole genome shotgun sequence.
ACCESSION   DAORHF010000061 DAORHF010000000
VERSION     DAORHF010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15715
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2829_length_15715_cov_5.246041"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..514)
                     /locus_tag="PKJ16_07190"
     CDS             complement(35..514)
                     /locus_tag="PKJ16_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HNV46810.1"
                     /translation="MSLGAGCLMVVLCILVPATAWADWLLLLHSEAGSRFGDNWEAKI
                     EVEGKFRDNMREYYDLEVMPWAAYRFTAWFKFGLGWRELVWRTNRAVYTKQVENDRGA
                     QTYSQVSGHYWQVEHRPLADFMFTVTAWLITIEERLRVEYRQIENQDAYFRCNYSAL"
     gene            786..1664
                     /locus_tag="PKJ16_07195"
     CDS             786..1664
                     /locus_tag="PKJ16_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46811.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTIGCSHNACTFCGMYKDKRFRVR
                     DLDEIKADIRMAKDSYGDVEKVFLCDGDAIEIDTPVLLDILAELRAAFPSLRHVASYV
                     GPASTLRKSPEELATLCAAGLRKAYLGVETGDDALLAEVRKGVNADEMLRAGRRLIEA
                     GMNLSSMVLLGLAGPGERSREHAIATANITNEMRPHYLAALTVTPVPGTVLYNRVQKG
                     EFTLLDPFETLEEMKVLFEHITIDNLRFVGIHASNYLPINGTLQRDREKMLATIDAAL
                     ANRDRAALRPDSMRGL"
     gene            1771..2361
                     /locus_tag="PKJ16_07200"
     CDS             1771..2361
                     /locus_tag="PKJ16_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46812.1"
                     /translation="MFIKRLFFKIPPRIRKACYRFLMYGSFLTFGEIFFYTLTKTGRL
                     IPFIGEIVFSYQWAVDDRLHLDAIWCAPISTLYGQASLWMVPVYGSICLFGIEPVYRL
                     THDAPRPLCWLPVRAFIYMIIILVMECAWGWIYYVLLDMKIWYYRDNLAVLQFTSLAI
                     APMWFLLGIIAEVFVRIVNDLDDKLEDLKRRITSVP"
     gene            2432..7705
                     /locus_tag="PKJ16_07205"
     CDS             2432..7705
                     /locus_tag="PKJ16_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46813.1"
                     /translation="MIRIPGYNVTEIVHQSPRTAVYRAYRESDNARVALKVLDPAFPA
                     PDEIARLNREYAIARHLDAPGIIGVYGLERIGDTCMIVMEDIGGESIAAAIKFSRPAL
                     AEALRLSIGIAEAIEGMHRANVIHKDINPSNIVWNRNTGTIRIIDFGTASLLPRERSY
                     IKNPNILEGTLAYLSPEQTGRLNRSIDYRTDYYSLGVTLYELFTGQRPFSAIEPLEMI
                     HCHIARQPNPPDSANGSIPPALSRIIMKLMAKTAEERYQSARSLRADLASCLEAAANP
                     DAPQDFPIAIDDVADRFQIPQNLYGREKDIATLLSVFDLARNGAAEVLMVRGYAGIGK
                     STLVYEIQKPIIQHRAYYITGGFDQLKRDMPYSALITAFRDLLRQILAEGDLEIAVWR
                     EKLLAALGMNAQIVIDVLPELSLIIGDQPAPPPLPLQESQNRFNLVFQRFVGSFADAR
                     HPLVIFLDDLQWADQASLDFIEYLCAHSAVRHLLLIGSYRDNEVGPVHPLRVMLKNIG
                     ESKTINDISLFPLDSAHVTALVADTLRCDHDTVRPLAQLCHHKTAGNPFFLSQFLHNL
                     YDEGLVRFDPAAGTWRWDEELICESGITDNVADLLARKFDTLPEETRRLLSIGACIGG
                     RFDLDTLSTIADRAPEETAKGLWDALKEEMIAASDDSYGSDPAVGRTTSYRFIHDNVY
                     QSITARLDPDERKRLNLRIGRLFADEMRASGRDTMIFDVVNHINFGVDLIEDPDERQR
                     LAELNLQAEKKAKNSAAYRAAYNYASTGLSLLAPDAWTSAYRLALTLHVEAAESAYLC
                     MDFETMERLTGTVIAQARSIIDAVDAYDVMIRSLITQNRIDEAVETGSTVLRKLGASI
                     PDVRRIGTVTILRYLIRVKLRLFRKNFEELSSFRDMTDPRVRSVIRIIYVFAGVVGRI
                     YPPSAFFLTLFTITQYLSHGYLPYTAYSFIYYAWISNYLFGSFDTASGYATVALNVLD
                     RYDAKVLKAKIYFFYYAFIMHWRYHLKSILKPLLEGYNLGLETGDFEFGGYCINVHLS
                     YQFFLGARLDELEESIEHFTDSLREIRQERACYINEIYRAACRTLRQGSSEPWALASE
                     NVDLNDVRGLFERTSDRNALFNMRFCEMLLAFLFGHIDHAERSADYLAKNADAMYSLP
                     NVPTAVFYATLVHLAAYETASPARRRQLRSVIRSNSAKIKKWMKGAPMNFMHRWYLVE
                     AERCRIDGRDLRAMELYDRAIESADQNEYLNDRAIANECAARFYVSRNRPKSARSYLV
                     EARHCYTHWGALAKVREIETRYGSIIATAEWPVTTIAPDSTPVTSTTTRCESLDLATI
                     MKISQALSSEIVLADLLTTLMNIAIESAGAQRGYLILEKNGTLYIEAEGAAGEAEIRV
                     LQSTPLVGAAPGGGSIILSESVLNFVLRTRETVIVRDASADPRFSRDEYIMTAMPRSI
                     MCLPFINQGKLGGILYLENNLIPDAFTAARVRMLELLSSQIAISIENARMYVELDDLN
                     KNLERKVEERTIELNRAYGMVKQKEAIIEQDLALAQSIQNKILPAPDEYCGVRVHVDY
                     RPMAKVGGDIYDIHPRSDTLLRVLIADATGHGVQAALVTMIIKSEYETLKDRIDGPAD
                     LLAALNNEFVSRYRSLNMFFTCVVVDIDTATRTLRYASAGHYDQYLVDGGLTVPITSG
                     GKTIGFIQDAHYRERSVSYAPGARLFLFTDGATEEFNDAREVYGEERLKSVIQSGADV
                     RGTIDSIIADIQSFVGDRGLDDDLTIIGIEL"
     gene            7712..8839
                     /locus_tag="PKJ16_07210"
     CDS             7712..8839
                     /locus_tag="PKJ16_07210"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV46814.1"
                     /translation="MPIVTIVKPAAGGLGLGFLDGRAVFVPSSDVGETLEIELVEEHA
                     DYARGAITRIIAPSDNRFDPECPNFGTCGGCDYLHITYEAEIAHKKTIALDCLRRLGR
                     FAPEALPVLEVEHGPRAGYRSHAELASDGVSLGFFARRSHRTVPLPPDGCRLLAEPIN
                     RALASLAPPPPGALKIALDAEGYVRRAPFDSVVREHENGICYRRSLQGFFQANRHLRG
                     RMIEIVRTHAHAVPEAPFADVYCGVGFFSVALARAGMRGEGFDGDAEAVRWARANASD
                     NECAGIEFFSRSADSIAPAALSGRTVIVDPPRAGLSKRFRRTLAASGAPRIIYVSCDP
                     ATFARDASAFAAAGFRFAAHTLIDMFPATAHIEQIALFESA"
     gene            8914..10365
                     /locus_tag="PKJ16_07215"
     CDS             8914..10365
                     /locus_tag="PKJ16_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV46815.1"
                     /translation="MPFPMLERIRQHFRNRWSNDGGYREVLTIAFPLILSTGSWSVQH
                     FVDRMFLAWYSPEAIAASMPAGILNFTVMSLFIGTASYVSTFVAQYHGSNQHEKIGPL
                     MWQGIYLSLIAGLVHFALMFAAEPIFVIVGHEPGVRELEVIYFTVLCTGAMPVVAASA
                     MSGFFSGLGKTWPIMWINVIATAVNLALDYVLIFGRWGFPEMGIRGAGIATVISGVVS
                     FAIYSALLLRPSYDRAFRTLRGWRPRIDLARRLVRFGLPSGVQFFLDVAGFTIFIILI
                     GRLGTVNLAASNIAFNINTLAFMPMIGFGITVSILVGQFLGNNRPDKAAYATYSGFHI
                     TFMYMAIVAALYVIAPGIFLEPFLDHARPDTTAIREIGTVLLRFIALYCLFDSASIIF
                     ASAIKGAGDTRFVMFTILAAAVCVLSVPCYLAIHYFGAGLYTTWTIGTAYVCLLGIVF
                     LLRFRGGKWKAMRVIEETPVLIPPAFPESPLSE"
     gene            complement(10375..11706)
                     /locus_tag="PKJ16_07220"
     CDS             complement(10375..11706)
                     /locus_tag="PKJ16_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNV46816.1"
                     /translation="MELKRQLGLTTAVLIIIADVIGTGIFMTTGEALAMTGSALGVLA
                     LFAVGGVIAVTGSLCYAELAAMWPDDGGEFVYLRRMFGPLPSFLTGWVSLFIAFSLAA
                     SMSAITAVNYINKITAEPLLTGIGVPLAGAGIVVIFSVVHALGVQRGSAVQNFFTVLK
                     IVIVFAFIALGFSFADWGAAGRLTAAHAVPGGGSVLSYGSALIIIMYAYSGWNGSTYI
                     AGEIKDPARNLPRALFWGAVAITLIYLALNAVYLLASPGADIIANGRYTIGALTAENL
                     FGKTVGPYFNIAIVIILVSSVSVQMMIGPRVTYAMARERAIFSALERVNPRLGTPDLA
                     ILVQTAIAVFYVFIGFDAILKMLIYMGFALSVFPFLTVAGMMHLRITRPDMPRPFRVP
                     FFPAVPLIYLALSAGVIVTTLIEKTVPSLFALGALALGALAYLAHRRFSAR"
     gene            11831..12454
                     /locus_tag="PKJ16_07225"
     CDS             11831..12454
                     /locus_tag="PKJ16_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV46817.1"
                     /translation="MIVEQILVTGMAVFCYLVADERTKTAALIDPAGDYDAIFEQINR
                     HGVTVRYIINTHGHFDHTSGNAHMLEKTGAELLIHEADAGMLGSIPNRVLSRVTGGKG
                     SPKAVRKLADGDVIEIGEVRLTVIATPGHTPGGICLYAPGHVFTGDTLFTEGMGRTDL
                     PGGSYKKIMESITGKILALPDDTIIWPGHHYGRFPKSTVKEQKRAYR"
     gene            12483..12941
                     /gene="yidD"
                     /locus_tag="PKJ16_07230"
     CDS             12483..12941
                     /gene="yidD"
                     /locus_tag="PKJ16_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HNV46818.1"
                     /translation="MRRFGITFALAAATIAASPIAPARADSFAPWKSDVRVGDATLAE
                     HRHDRERHIHRHAHASPPASSPIRRGYRTVYSGPQGGAFFLIRFFQMVISPQDGPNCR
                     YNPTCSGYGRQAVEMHGGFVGALLAGDRILRCNPYAPIGDDPVPRAITGK"
     gene            12943..13869
                     /locus_tag="PKJ16_07235"
     CDS             12943..13869
                     /locus_tag="PKJ16_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46819.1"
                     /translation="MSVRTPGPALLCLALCATLAVPLFAQPARHETRDNFFVAQRRLI
                     WQHFNGGRHFDCIAETRRLLAYQHLTPVDRAPYEYFIEVNYFLGGQYRTVAAKAGSSD
                     GRGSYSIATSILASQALYRLGRQDAALGELARWDYAGLTERDRREVFFRRSEILVRDY
                     RYQQLQDEIAQARRHLPDSSSAPMSDAVARHRDAGRKMPWLAAVMSAALPGSGQAYAG
                     KYAAGLISLAAIAVTGYGAYHFHSRGEWNLAYTCGFFAALFYGGTVYGAYNAALNANR
                     TADDAFNQAFIKNHLPAYRPASFMERPSGVRP"
     gene            13866..14795
                     /gene="bamD"
                     /locus_tag="PKJ16_07240"
     CDS             13866..14795
                     /gene="bamD"
                     /locus_tag="PKJ16_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HNV46820.1"
                     /translation="MSRRPQHAAGAAICARTWRYRFRHAFPFALAVSLALTTLAARPL
                     AAGERELITLAEMHLRNREYHNAVTELMRYQHLHPLGALYPRSLLLMSTAYFEGNNYT
                     RAIDTARLCYDRFRNNPEGEEALYRLGHMRLLTGSAFMSFSTFDEYMALYPQGRFSET
                     VLAERPYAHLLAFEIDDAKSEIENYRRQYPAGAYLAAMDELDGLIAAEEGRPKKKMWV
                     AVLGSVFIPGFGNFYTENYWTGLLSFLSNGVLIYLIYDAAVHRDTFRMVFFGLLELSF
                     YQWSIISAVNNVYEYNYPEDFRRGIRLSLSKRF"
     gene            14843..15511
                     /locus_tag="PKJ16_07245"
     CDS             14843..15511
                     /locus_tag="PKJ16_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46821.1"
                     /translation="MDDFLENPEDVLAAEEEERRRYSTYYKTIEGMLKENRPLDEIAD
                     NIISGTFEDFDIQKKDSIDKFLDFVFLKVKTGVPHIISLAYPAMRITDSQLEDKIEKL
                     INLYLHPEIIFTLLKFFTRNIHNSDTNLYLANLITSEDIIRSLFDTFALFKADIAIVD
                     PNRRTLNVKRLQQFHPGTDNRFSSPLDAAARFKYILEFISLKQNTGHIFTRDNLQLTG
                     PGRG"
BASE COUNT         2991 a         5306 c         4538 g         2880 t
ORIGIN      
        1 tacaggggtt gtttgaaaac aggccattta tgcctcaaag tgctgaatag ttacatcgaa
       61 agtatgcgtc ctggttttcg atctgccgat actcgacgcg caggcgctcc tcgattgtga
      121 tgagccatgc cgtgaccgtg aacatgaaat cggccagcgg acgatgctcc acctgccagt
      181 aatgtcccga aacctgtgaa tatgtttgag cgcctctatc gttttcgacc tgtttcgtat
      241 acaccgcccg gttcgtccgc cacaccagct cccgccagcc caggccaaac ttgaaccatg
      301 cggtgaagcg gtaggccgcc cagggcatga cctcaaggtc gtaatattcc cgcatgttgt
      361 cgcggaactt gccctcgacc tctatctttg cctcccaatt atcgccgaac ctgctcccgg
      421 cctcgctatg caatagcaaa agccaatcgg cccaggccgt tgccggaaca agaatgcaca
      481 gaacgaccat gagacagccc gctcccaaac tcattcgatc ctcgtcggcg acactccttc
      541 gaacatttaa atatgaacat ttttgtaata aaaatcaagg gcttttttcc taatgttaaa
      601 aaatcatctt cccgcatcca ccatgcagta atcattcatg ttgatagatg agcgcccgaa
      661 tcgcgatcat cgtcgccgaa aaaacatttg ccctaaacgc gacatcgacg atcatgaccc
      721 acgcgagcgg gcatgcgcga cagcgctcca tcgcaataat ccgaaccgac attcatggga
      781 ccatcatgcg gtacgaagga aacatcttcc gccccttcag cgaggcgaac agctatctgc
      841 tgcagtgcac catcggctgc tcgcacaatg cgtgcacctt ctgcgggatg tacaaggaca
      901 agcgctttcg cgtccgggat ctcgacgaga tcaaggccga cattcgcatg gccaaagatt
      961 cctacggcga cgtggagaag gtctttctgt gcgacggcga tgcgatcgag atcgacacgc
     1021 ccgttcttct ggacatcctc gcggagctcc gcgcggcgtt cccgtcactg cgccatgtgg
     1081 caagctacgt ggggcccgca agcacgctcc ggaaatcgcc cgaagagctc gcgacgctgt
     1141 gcgcggccgg gttgcgcaag gcctacctcg gcgtggagac cggcgacgac gcgctccttg
     1201 ccgaggtccg caagggcgtg aacgcggacg agatgcttcg tgccgggcgg cgcctcatcg
     1261 aggcggggat gaacctttcg tccatggtgc tcctgggtct cgccgggccg ggggagcgat
     1321 cgcgggagca cgccatcgcc acggcgaaca tcaccaatga gatgcggccg cactatctcg
     1381 ccgccctcac ggtgacgccc gtgccgggaa cggtgctcta caatcgggtg cagaaaggcg
     1441 agtttacgct cctcgacccc ttcgagaccc tcgaggagat gaaggtgctt ttcgagcaca
     1501 tcaccatcga caatcttcgc tttgtgggca ttcacgcatc gaactatctt cccatcaacg
     1561 gcaccctgca gcgcgaccgg gaaaaaatgc tcgcgaccat cgacgcggcg ctggcaaacc
     1621 gcgaccgtgc ggcgctgcgg cctgactcga tgcggggact ctagcggggt cccgcaaata
     1681 cgcgagttca tttctgaaaa gtgattgaat tttccctgag cggcccgcac ggtggggcga
     1741 atcgtcattt catcgacaag gacgtccgga atgttcatta aacgcctctt ttttaagata
     1801 ccgccccgca tcaggaaggc ctgctatcgc tttctcatgt acggcagctt ccttaccttc
     1861 ggcgagattt tcttctatac gctcactaaa accggccgac tcattccctt catcggggaa
     1921 atcgtgttct cgtatcagtg ggcggtggac gatcggctgc acctggacgc aatctggtgc
     1981 gcgcccatct cgaccctcta tggacaggcc agcctgtgga tggtccccgt gtacggcagc
     2041 atttgccttt ttggcatcga acccgtgtat cgcctcaccc acgacgcgcc gcgcccgctg
     2101 tgctggctgc cggtccgggc gttcatctac atgattatca ttctggtcat ggaatgcgcc
     2161 tggggatgga tctactacgt tctgctcgac atgaagatat ggtattatcg cgacaatctg
     2221 gcggtgctgc agttcaccag cctcgccatc gcgcccatgt ggttcctgct gggaatcatc
     2281 gccgaggtgt tcgtacggat cgtcaacgat ctcgacgaca agctcgaaga cctgaagcga
     2341 cggatcacgt ccgtcccata gacgcgatga ggacgacccg ccgtcatcac cgatatcccc
     2401 gggtcggcat tccgaccctt cggcggcgcc catgatacgc atccccggtt ataacgtcac
     2461 ggagattgtc caccagagtc cccggaccgc cgtatatcgc gcataccgag aatcggacaa
     2521 tgcccgcgtg gcgctcaagg tcctcgaccc cgccttcccc gctcccgacg aaatcgcgcg
     2581 gctcaatcgc gaatacgcca tcgcgagaca tctcgacgct cccggcatca tcggcgtcta
     2641 cgggttggag cggatcggcg acacctgcat gatcgtcatg gaggacatcg gcggcgagtc
     2701 gatcgccgcg gcgatcaagt tttcgcggcc agcgcttgcg gaggccctgc gcctttcgat
     2761 cggtatcgcc gaggcgatcg agggcatgca ccgcgccaac gtgattcaca aggacatcaa
     2821 cccctccaat atcgtctgga accgcaacac cgggacgata cgcatcatcg acttcggcac
     2881 cgcctcgctc cttccgcgag agcgatcgta tataaagaat ccgaacattc tcgaggggac
     2941 cctcgcctat ctctcgccgg agcaaaccgg ccgcctcaat cgctcgatcg actaccggac
     3001 cgattactac tcgctcggcg tgaccctcta cgaactcttc accggccagc gccccttcag
     3061 cgcgatcgag ccgctcgaga tgatccactg ccacatcgcc cggcagccca atccgcccga
     3121 cagcgcgaac ggctcgatcc cgcctgcgct ctcgaggatc atcatgaagc tgatggcgaa
     3181 gaccgccgag gagcgctatc agagcgcgcg cagtctccgt gcggacctcg cctcctgcct
     3241 cgaggcggcg gccaatccgg acgcgcccca ggactttccc atcgcaatcg acgacgtggc
     3301 cgatcggttc cagatcccgc aaaacctgta cggacgggag aaggacatcg cgacgctcct
     3361 gtccgtcttc gacctcgcgc gcaacggcgc cgccgaggtg ctgatggtgc gcggctacgc
     3421 cggcatcgga aaatcgacgc tggtctacga aatacaaaaa cccatcatcc agcaccgggc
     3481 ctactacatc acgggcggtt tcgaccagct caagcgggac atgccgtact ccgcgctcat
     3541 caccgccttc cgggacctcc tgcgccagat cctcgccgag ggcgatctcg agatcgcggt
     3601 atggcgggag aagctcctcg ccgccctcgg gatgaacgcg cagatcgtca tcgacgtcct
     3661 ccccgagctc tcgctcatca tcggcgacca acccgcgccg ccgccgctgc cgctgcagga
     3721 gtcgcagaac cgcttcaatc tggtgttcca gcgcttcgtc ggctcgttcg cggacgcgcg
     3781 ccatccgctg gtcatctttt tggacgatct gcaatgggcg gaccaggcct ccctggattt
     3841 catcgagtac ctctgcgccc actcggcagt ccggcacctg ctgttaatcg gatcgtatcg
     3901 cgacaacgag gtcggcccgg tccatccgct gcgggtcatg ctcaagaaca tcggcgaatc
     3961 caagacgatc aacgacatca gcctcttccc gctggatagc gcgcacgtaa ccgcgctggt
     4021 cgccgacacc ctccgctgcg atcacgacac cgtgcggcca ctcgcgcaac tctgccacca
     4081 caagaccgcg ggcaatccct tctttctctc gcagtttctc cacaacctgt acgatgaagg
     4141 cctcgtccgt ttcgatcccg ccgcggggac ctggcgatgg gacgaggaac tgatatgcga
     4201 aagcggcatc accgacaacg tcgccgacct ccttgcgcgc aagttcgaca cgctcccgga
     4261 ggaaacgcgg cgcctgctct cgatcggcgc ctgcatcggc ggccggttcg acctggacac
     4321 gctctcgacc atcgccgacc gtgcgcccga ggagaccgcc aaaggattgt gggacgcgct
     4381 caaggaggag atgatcgccg cgtccgacga ctcctacgga tccgaccccg cggtgggccg
     4441 cactacgagc tatcggttca ttcacgacaa tgtctatcag tccatcaccg cgcggctcga
     4501 tcccgacgag cggaagcggc tcaacctccg catcgggcgc ctgttcgccg acgagatgcg
     4561 ggcgtccggc cgcgacacaa tgatcttcga cgtggtcaac cacatcaact tcggcgtcga
     4621 tctcattgag gaccccgatg agcgccagcg cctggccgag ctcaacctgc aggcggaaaa
     4681 aaaggccaag aattcggcgg cctatcgcgc ggcctataac tacgcctcga ccggactgtc
     4741 cctcctcgca ccggacgcct ggacgagtgc gtaccggctc gcgctcaccc tgcacgtcga
     4801 agcggccgaa tcggcgtacc tgtgcatgga tttcgaaacc atggagcgcc tgacggggac
     4861 cgtcattgcg caggcacgct cgatcatcga cgcggttgac gcctacgacg tgatgatccg
     4921 ctcgctcatc acccagaacc ggattgacga ggccgtcgag accggcagca ccgtgctgcg
     4981 aaagctcggc gcttcgattc cggacgttcg ccggatcggc accgttacca ttctgcgtta
     5041 tttgatccgc gtgaagctgc gcctgttccg gaagaacttc gaggaattgt catcattccg
     5101 cgacatgacg gacccgcgcg tccgttcggt gatacgcatc atttacgtgt tcgcgggcgt
     5161 tgtgggccgg atatatcccc cctccgcgtt ctttctcacg ctgttcacca ttacccagta
     5221 cctctcgcac gggtacctgc cctacaccgc ctactcgttc atctattatg catggatcag
     5281 caattacctc ttcgggagct tcgatacggc cagcggttat gcgacggtcg ccctcaacgt
     5341 gctcgaccgg tacgacgcga aggtcctcaa ggcgaagatc tactttttct actacgcctt
     5401 catcatgcac tggcgctatc atctcaagag catcctcaag ccgctgctcg aaggatacaa
     5461 ccttggcctc gaaaccggcg atttcgagtt cggcggatac tgcatcaacg tgcatctttc
     5521 gtatcagttc ttcctggggg cgcggctcga cgagctggag gaatcgatcg agcatttcac
     5581 cgattcgctg cgcgagatcc ggcaggagcg cgcctgctac atcaacgaga tctaccgggc
     5641 ggcctgccgc accctcaggc aggggtcctc cgagccctgg gcgctcgcca gcgagaacgt
     5701 cgacctgaat gacgtccgcg gccttttcga gcggaccagc gaccggaacg ccctgttcaa
     5761 catgcggttc tgcgagatgc tgctcgcctt cctcttcggc cacatcgacc atgcggagcg
     5821 ctccgccgac tatctcgcga agaacgccga tgcgatgtat tcgctgccga acgtgcccac
     5881 ggcggtcttc tacgccacgc tggtccatct ggccgcctac gagaccgcgt cgcccgcacg
     5941 ccgtcgccag ctccgttcgg tgatccgctc gaacagcgca aaaatcaaga agtggatgaa
     6001 gggcgcgccg atgaacttca tgcaccgctg gtacctcgtc gaggcagagc gctgccgcat
     6061 cgacggtcgc gacctgcgcg cgatggagct gtacgaccgg gcgatcgaat ccgccgacca
     6121 gaacgaatac ctcaatgacc gcgccatcgc gaacgagtgc gccgctcgat tctacgtgag
     6181 ccgaaaccgg ccgaagtccg cacgctcgta cctggtcgag gcgcggcact gctacaccca
     6241 ctggggggcg ctggcgaagg tgcgcgagat cgagacgcgc tatggctcca ttatcgccac
     6301 ggccgaatgg ccggtcacga ccatcgcgcc cgactccacg ccggtcacct cgacgacgac
     6361 gcgctgcgaa tcgctcgacc tcgcgacgat catgaagatc tcgcaggccc tctccagcga
     6421 aatcgtcctc gccgatctgc tcacgacgct catgaacatc gctatcgaga gcgccggggc
     6481 ccagcgcggc tacctcatcc tcgagaaaaa cggcacgctc tacatcgagg ccgagggcgc
     6541 tgccggcgag gccgagatcc gggtgctgca atcaacgccg ctcgtcggcg ccgcgccggg
     6601 aggggggtcg atcattcttt ccgagtcggt cctcaacttc gtgctgcgca cccgcgagac
     6661 cgtgatcgtg cgcgacgcat cggccgaccc ccgcttcagc cgcgacgaat acatcatgac
     6721 cgccatgccc cgttcgatca tgtgcctgcc gttcatcaac cagggaaagt tgggcggcat
     6781 actctacctc gagaacaacc tcatccccga cgccttcacc gcggcgcgcg tgcggatgct
     6841 ggagctgctc tcctcgcaga tcgccatctc catcgagaac gcgcgcatgt acgtcgagct
     6901 cgacgacctc aacaaaaacc tcgagcggaa ggtcgaggag cgaaccatcg agctcaaccg
     6961 ggcgtacggc atggtcaagc agaaggaagc gatcatcgag caggacctcg cgctggcgca
     7021 aagcatccag aacaagatac tgcccgcgcc cgacgaatat tgcggcgtcc gggtgcatgt
     7081 cgactaccgc cccatggcca aagtcggcgg cgacatctac gatatccatc cccgttccga
     7141 cacgctgctg cgggtgctga tcgccgacgc gaccggccac ggcgtgcagg ccgccttggt
     7201 caccatgatc atcaagagcg aatacgaaac gctcaaggac cggatcgacg gacccgccga
     7261 tctcctcgcc gcgctcaaca acgagttcgt gagccgctac cgcagcctca acatgttctt
     7321 cacctgcgtg gtggtggaca tcgacaccgc gacgcggacg ctccgctatg ccagcgccgg
     7381 ccattacgac cagtatctgg tggacggcgg cctgaccgtt ccgatcacct ccggcggcaa
     7441 gaccattggt tttatccagg acgcgcacta ccgcgaacgt agcgtgtcct atgcccccgg
     7501 tgcgcgattg ttcctgttca ccgacggcgc caccgaggaa ttcaacgacg cgcgcgaggt
     7561 gtacggcgag gagcgcctca agtcggtgat tcagtccggc gccgacgttc gcggaaccat
     7621 cgactccatc atcgccgaca tccaatcctt cgtcggggac cgcggcctgg acgacgacct
     7681 caccatcatc ggtatcgagt tgtagccgcc catgccgatt gtcaccatcg tgaagcccgc
     7741 tgccggcgga ttggggctcg gtttcctcga cgggcgcgcc gtgttcgtcc cgtcgagcga
     7801 tgtcggcgaa accctcgaga tcgaactcgt cgaggagcat gccgattacg cacgcggcgc
     7861 aatcacgcgc atcatcgcgc cgtcggacaa tcgcttcgat cccgaatgcc cgaacttcgg
     7921 gacctgcggc gggtgcgatt atctccacat cacctacgag gcggagatcg cgcacaagaa
     7981 gacgatcgcg ctcgattgcc tgcggcggct cgggcggttt gcgcccgagg ccctccccgt
     8041 gctggaggtc gagcacggac cgcgcgccgg ctatcggagc cacgcggagc ttgcgagcga
     8101 cggcgtatcg ctcggctttt tcgcgcggcg ctcgcaccgg acggttcccc tcccgccgga
     8161 cggttgccgc ctcctcgcgg aacccatcaa ccgggcgctc gcctcgcttg caccgccgcc
     8221 gccgggagcg ctcaaaatag ccctggacgc ggaagggtac gttcgccgcg cgccgttcga
     8281 ttcggtcgtt cgcgagcacg agaacgggat ctgctaccgg cgatcgctcc agggcttctt
     8341 ccaggcgaac cgtcatctcc gggggcgcat gatagagatc gtgcgtaccc atgcacatgc
     8401 ggtcccggag gcgccctttg cggacgtcta ttgcggcgtc ggcttttttt ccgtcgcact
     8461 cgcgcgcgcg ggcatgcgcg gcgaaggttt cgacggggac gccgaggcgg tgcgttgggc
     8521 gcgcgcgaac gcgtcggaca atgagtgcgc ggggatagag tttttctcgc gttccgcgga
     8581 ttccatcgcg ccagcggcgc tttccggccg gaccgtcatc gtcgatccgc cgcgcgcggg
     8641 gctctcgaag cggtttcgcc gcacgctcgc cgcatccggc gccccgcgga tcatctacgt
     8701 gtcgtgcgat ccggcgacct tcgcgcggga cgcctccgcg tttgccgccg cgggcttccg
     8761 cttcgccgcg cacacgctca tcgacatgtt tcccgcaaca gcgcatatcg agcagatcgc
     8821 tctcttcgaa tcggcctgat ccgtccgcgc cggtcgcgcg aacgcgccat catcacccat
     8881 ttctacttgt aaaaaatacc gccgcttagt cccatgccat ttcccatgct cgagcgaata
     8941 cgacaacatt tccgcaatcg gtggtccaac gacggcggct accgcgaagt cctcaccatc
     9001 gcctttccgc tgatcctgag caccggctcg tggtcggtgc agcacttcgt cgaccgcatg
     9061 ttcctcgcct ggtactcgcc ggaggcgatc gcggcgtcca tgccggcggg gatcctcaac
     9121 ttcacggtga tgagcctgtt catcggcacg gcgagctacg tgagcacctt cgtcgcgcag
     9181 tatcacggat ccaaccagca cgagaagatc ggccccctca tgtggcaggg catctacctg
     9241 tcgctcattg cggggctggt tcacttcgcc ctcatgttcg cggcggaacc gatcttcgtc
     9301 atcgtcgggc acgagccagg cgtacgggag ctggaggtga tctacttcac cgtgctgtgc
     9361 accggggcca tgccggtggt cgccgcgtcg gcgatgtcgg gatttttctc cggcctggga
     9421 aagacctggc ccatcatgtg gatcaacgta atcgcgacgg cggtaaacct ggcgctcgac
     9481 tatgtgctca tcttcgggcg atgggggttt cccgagatgg gcatccgcgg cgcgggcatc
     9541 gccacggtga tctccggcgt cgtctcattc gcgatctatt cggcgctcct cctgcgcccg
     9601 tcctacgacc gcgccttccg caccctgcgc ggctggcggc cgcggatcga cctggccaga
     9661 cggctggtcc gttttggact ccccagcggc gtgcagttct ttctcgacgt tgcggggttc
     9721 accatcttca tcatcctcat cgggcggctg ggcacggtaa atttggccgc cagtaatatc
     9781 gccttcaaca tcaatacgct ggcctttatg cccatgatcg gcttcggcat cacggtctcc
     9841 atcctggtgg gccagttcct gggaaacaac cgcccggaca aggccgccta tgcgacctac
     9901 tccggcttcc acatcacctt catgtacatg gcgatcgtcg cggcgctgta cgtgatcgcg
     9961 cccggcatct ttctggagcc cttcctggac cacgcgcggc ccgacaccac ggccatccgc
    10021 gagatcggga cggtcctgct tcggttcatc gcgctgtatt gcctcttcga ttcggcgagc
    10081 atcatcttcg cctcggccat caagggcgcg ggcgacacgc gcttcgtcat gttcaccatc
    10141 ctcgccgcgg cggtgtgcgt actctcggtc ccctgctacc tggcgatcca ttatttcggg
    10201 gcgggcctgt acacgacctg gaccatcggc acggcctatg tgtgcctgct gggtatcgtg
    10261 ttcctgttac gctttcgcgg agggaaatgg aaggcgatgc gggtcatcga ggagacgccg
    10321 gtgctgatcc ctccggcgtt ccccgaatcg cccctgtccg aatagccgtc gcggctatcg
    10381 cgccgaaaaa cgccggtgcg cgaggtaggc cagcgcgccg agcgcgagcg cgccaagggc
    10441 aaagagcgac ggcacggtct tctcgatgag cgtggtcacg atcacccccg cgctgagcgc
    10501 aagatatatg agcggaacgg ccggaaagaa cggcacccga aacgggcgcg gcatgtccgg
    10561 ccgcgtgatc cgaagatgca tcatgccggc caccgtgagg aacggaaaga ccgacagcgc
    10621 gaagcccatg tagatgagca tcttgaggat ggcgtcgaac ccgataaaca cgtagaacac
    10681 cgcgatggcc gtctggacca ggatcgcgag gtcgggcgtc cccaggcgcg ggttgacgcg
    10741 ctcgagcgcg ctgaagatgg cgcgctcccg cgccatggcg taggtcacgc gcgggccgat
    10801 catcatctgc accgacaccg acgagacgag gatgatgacg atggcgatgt tgaaatacgg
    10861 gcccaccgtc tttccgaaaa ggttctcggc cgtgagcgcg ccgatcgtgt accggccgtt
    10921 cgcgatgatg tcggccccgg ggctcgcgag caggtaaacc gcgttaagcg ccaggtagat
    10981 cagggtgatc gcgaccgccc cccagaagag cgcccggggc aggtttcgtg ccggatcctt
    11041 gatctcaccg gcaatgtagg tcgatccgtt ccatcccgaa taggcgtaca tgatgatgat
    11101 gagcgccgag ccgtacgaaa gaacggagcc gccgcctgga accgcatgcg ccgccgtgag
    11161 ccggcccgcg gcgccccaat cggcgaagga gaagccgagc gcgatgaagg cgaacacgat
    11221 gacgatcttt aggaccgtga agaagttctg cacggcgctt ccccgctgaa cgccaagcgc
    11281 gtgcaccacg ctaaagatta ccacgatgcc cgccccggcg aggggcacgc cgatcccggt
    11341 caagagcggc tcggccgtga tcttgttgat gtagttgacc gcggtgattg ccgacatcga
    11401 cgccgcgagc gagaaggcga tgaacagcga cacccacccg gtgagaaacg acggcagcgg
    11461 gccaaacatc ctgcgaaggt agacgaactc cccgccgtcg tcgggccaca tggcggccag
    11521 ctcggcgtag cacagcgatc cggtgaccgc gatgacgccg cccaccgcga agagcgccag
    11581 cacgcccaac gccgagccgg tcatggcgag cgcctcgccg gtggtcatga atatccccgt
    11641 cccgatcacg tccgcgatga tgatgagaac ggccgtcgtg agtcccagct gtcgtttgag
    11701 ctccatgcgc gcatcccctt cgcccgccgc cggcggtgaa ataaatttct tgaaatatct
    11761 gcaaccgtca caccgaatag ccccaatcgt ttcctgtaaa gtacatttcc gcttcggggg
    11821 caatcacggc atgatagtcg aacagatcct ggtcacgggc atggcggtct tctgctatct
    11881 cgtcgcggac gagcggacaa agaccgccgc cctcatagat cccgcgggcg actacgacgc
    11941 gattttcgag caaatcaacc ggcatggcgt gaccgtccgg tatatcatca acacgcacgg
    12001 ccacttcgac cacacctcgg gcaatgcgca catgctcgaa aagaccggcg cggagctttt
    12061 gatccacgag gccgacgccg gcatgctggg aagcattccc aaccgggtcc tctcccgcgt
    12121 caccggcggc aagggctctc cgaaggccgt ccgaaaactc gccgacggcg acgtcatcga
    12181 gatcggcgag gtccgcctga ccgtcatcgc cacgccgggc cacaccccgg gcggcatctg
    12241 cctgtacgcg ccgggccatg tcttcaccgg cgacaccctc ttcaccgagg ggatggggcg
    12301 caccgacctg cccggcggat catacaagaa aatcatggaa tccataaccg ggaagattct
    12361 cgccctcccc gacgatacga tcatctggcc gggacatcac tacgggcgct tcccgaaatc
    12421 gaccgtgaag gagcaaaagc gcgcataccg ctgagcgcgc gccatcaccg gacatgatac
    12481 gcatgcgccg attcggcatc acattcgcac tcgccgccgc gacgatcgcc gccagtccga
    12541 tcgcgccggc gcgcgccgac tccttcgctc cgtggaagag cgacgttcgc gtgggcgacg
    12601 caaccctcgc ggagcaccgg cacgatcgcg agcgccacat ccatcgccat gcgcacgcgt
    12661 ccccgccggc atcatcgccg atccgccgcg gataccggac ggtgtacagc ggtccgcagg
    12721 gcggcgcgtt tttcctcatc aggtttttcc aaatggtgat atcgccgcag gacggcccca
    12781 actgccgata caacccgacc tgcagcggct acggccgcca ggcagtcgag atgcacggcg
    12841 gtttcgtggg cgcccttctc gccggcgacc gaatcctacg ctgcaacccg tacgccccca
    12901 tcggcgacga tccggtcccg cgcgccatca ccggaaaata gcatgtccgt acgaacaccg
    12961 ggaccggccc tgctgtgcct cgcgctctgt gcgaccctcg cggttccgct tttcgcgcaa
    13021 ccggcccgtc acgaaacccg ggacaacttc ttcgtcgcgc aacgacggct catctggcag
    13081 cacttcaacg gcggacggca cttcgactgc atcgccgaga cccggcggct cctcgcctat
    13141 caacacctga cgccggtgga ccgggcgccc tatgagtact tcatcgaagt gaactacttt
    13201 ctgggcgggc aatatcgcac ggtggccgcg aaggcgggat catccgacgg acgcgggagc
    13261 tactcgatcg ccacttcgat tctcgcgtcc caggccctgt atcgactggg ccgccaggac
    13321 gctgcgctgg gcgagctggc gcgatgggac tatgcgggcc tcacggaacg tgaccgacgc
    13381 gaggtcttct tccggaggag cgagattctg gtgcgcgact atcgctatca acaactccag
    13441 gacgagatcg cacaggcccg gcggcacctt cccgatagtt cgtccgcccc catgagcgac
    13501 gcggttgcgc gtcatcgcga cgcggggagg aagatgccct ggctcgcggc ggtcatgtcg
    13561 gcagcgcttc cgggaagcgg acaggcctat gcgggcaaat atgccgccgg cctcatcagc
    13621 ctggcggcga tcgccgtgac cggctacggc gcctatcact tccatagccg cggtgagtgg
    13681 aacctcgcct acacctgcgg cttcttcgcc gcgctctttt acggggggac cgtgtacggc
    13741 gcctacaatg cagcgctcaa cgccaaccga acggccgatg acgccttcaa ccaggcgttc
    13801 atcaaaaacc acctgcccgc ctatcgtccc gcctcattca tggagcgccc gtcgggggtg
    13861 cggccgtgag ccgtcgcccg caacacgcgg caggtgcggc catctgcgcg cgcacatggc
    13921 gatatcgatt ccgccacgca ttcccgttcg cactcgcggt ttcgctggcg ctcacgacac
    13981 tcgcggcccg tcccctcgcg gcgggcgagc gcgagctgat caccctcgcc gagatgcacc
    14041 tccgcaaccg ggaataccac aacgccgtga ccgagctcat gcgctatcag caccttcatc
    14101 ccctgggcgc gctctatccg cgcagcctgc tcctgatgag cacggcatac ttcgagggca
    14161 acaactacac ccgcgcaatc gacaccgcgc ggttatgcta cgaccgattt cgcaacaacc
    14221 cagagggcga ggaggccctc taccgcctgg gacacatgcg cctgctcacg ggttcggcgt
    14281 tcatgtcgtt ttccacgttc gacgaataca tggcgctgta tccgcagggg cgcttcagcg
    14341 agaccgtgct ggccgagcgc ccgtacgcgc acctgctcgc tttcgaaatt gacgacgcca
    14401 agtccgagat cgagaactac cgtcgccagt accccgcggg cgcctatctg gccgccatgg
    14461 acgagctcga tgggctcatt gccgcggaag agggccggcc taaaaagaag atgtgggtcg
    14521 cggtgctggg ctcggtgttc attcccggtt ttggaaactt ctatacggag aattactgga
    14581 ccggtctcct ctcgttcctt tcgaacggcg tcctcatcta tctgatctac gacgccgccg
    14641 tgcaccgcga cacattccgg atggtgttct tcgggctgct cgagctctcg ttttaccagt
    14701 ggtccatcat ctcggcggtg aacaacgtat acgaatacaa ctaccctgag gattttcggc
    14761 gggggatacg gctgtcgctc tcgaagcgtt tttagtttgc attttcggga ttgcgggata
    14821 caaacggcac aggggcaccg gcatggatga ttttctcgaa aacccggagg acgtgctcgc
    14881 ggccgaggag gaggagcgca gacgctactc aacctactac aaaaccatcg agggcatgct
    14941 caaggagaac cgccccctcg acgagatcgc cgacaacatc atcagcggca ccttcgagga
    15001 cttcgacatt cagaagaagg actcgatcga caagttcctc gacttcgtgt ttctcaaggt
    15061 caaaaccggc gttccgcaca tcatcagcct cgcctatccc gccatgcgga tcaccgacag
    15121 ccagctcgag gacaagatcg aaaagctcat caacctctac ctccacccgg agatcatctt
    15181 caccctgctc aagttcttca cccgcaacat ccacaattcc gacacgaacc tgtacctggc
    15241 gaacctcatc acctccgagg acatcatccg atcgctcttc gacaccttcg ccctcttcaa
    15301 ggccgatatc gccatcgtgg acccgaaccg gcgcaccctg aacgtcaaac ggctccagca
    15361 attccatccg ggcacggaca accgcttctc gtcgcccctg gacgccgcgg cgcggttcaa
    15421 atacattctg gagttcatat cgctcaagca gaacaccggg cacatcttca ctcgggacaa
    15481 cctccagctc accggtcccg ggcgaggata ggcgcgcagg accggcccag gcgcgcgcat
    15541 gcgatcgaag gggttgcggt cggctacatg ccgacgcggg ggattttttc gatgacgcta
    15601 tcgtcgctca taatcagctc gcgcacgtag acgttcttat ggcgcccggt gtgccggatc
    15661 ttcatgaagt cgttgaattc cggctcgtag cttctgatga gccagaagag gatgt
//
LOCUS       DAORHF010000062        15714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2831_length_15714_cov_5.342167, whole genome shotgun sequence.
ACCESSION   DAORHF010000062 DAORHF010000000
VERSION     DAORHF010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2831_length_15714_cov_5.342167"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2343)
                     /locus_tag="PKJ16_07250"
     CDS             complement(<1..2343)
                     /locus_tag="PKJ16_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV46822.1"
                     /translation="MILGIDAGSKFIKVTSVSDNTVRHIDYREHHGDPAGAASTLLAE
                     IPNAPAIACTGLFGGHIAERFPRAVQVDEVSCIIAALRHREARAHAVLAVGAASVKLI
                     TLDGEGRFASYAENSLCAAGTGSFLDQQMKRMGFSYDDIGALPFVPDPPEIATRCAVF
                     AKSDLIHRQQEGHSRAALWSGLCRGVVMTMLHAAPRLDDEAVLMCGGLFLNPAVRRFT
                     EELLEGARFIDEGHFLAALGAAIQLRDGGGRHRPVGARGVARAEGARYAGSLSLARSR
                     PAASTAIREYADDGNEIRMHAPVVDGGSALLGIDIGSTSTKCALLDASTRTVLVDIYR
                     KTGADPIDAARRLFRALESALAGTDLRIVAAGTTGSGRRLIGEIIGAEVIVNEITAHF
                     RGATHFQPDVETIFEIGGQDAKYIRGHNGSVIDAAMNYVCAAGTGSFIEEQAERLGFD
                     VRTLGELVLGLSTPNTSDRCTVFMEQDIAQLLRNGYSREEAMAGVIRSIAKNYLNRVV
                     GARPVSGERIFFQGATARNRGLVAAFESLTGKEIVVSPYCHVMGAFGAALIAGDRTGA
                     GVGTFRGRGVFEREIATSHETCDRCANYCRITVARVEGSAVASWGYRCGKEADDPAPA
                     ARRTDHFARVARLGASCDAANGGARVVETRRPVAAIPEALSMHTYLPLWRTFLHELGF
                     ETVVEGGGGAELKEAAAQVTDADYCYPVKVALARFGRCAERDDAEYVFFPSVMSETRQ
                     DNGKPRVFCPYVISYPSLARSLFGGRREIIAPAIDFRFGDD"
     gene            2575..3171
                     /locus_tag="PKJ16_07255"
     CDS             2575..3171
                     /locus_tag="PKJ16_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46823.1"
                     /translation="MMIAHAAITRLPFNAAALPKGIVCTGRVVDGARWTDSTGENIVV
                     LCQTGAFKSPKGDSEDSRDAELSAHQYVRSAPAWRRVWSIRDFERNCPLDLTATFIPG
                     ALTVTDLDGNGIAEVTVLYKHACRGDVSPAGMKLIMREGAKKYAIRGIMRISPEIDPS
                     GRGEMNLDPTFAKAPRSFRDFAIQHWKKHEIEKSFEQP"
     gene            complement(3232..4344)
                     /locus_tag="PKJ16_07260"
     CDS             complement(3232..4344)
                     /locus_tag="PKJ16_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46824.1"
                     /translation="MSSNDTCITDRDEIERIIDTIFTNRDLFLRMDDVNQQVEMKEYR
                     DGSVTLRTLERLSTADPCVLFARLDDKLYCARITPTFDPSMAVVVDNRFEYPFSLQMV
                     QVITAARREERTSLASPSAGERPAIIITNLVSDTLLKDCFTAGRDWVEYFKDAIRGKF
                     TDRYSFARVCFLHERFADERMKFFYRERTPLYIENLRETPATEAGKTLYQFYKITIFQ
                     KDKGLNTRTTVSEASVPLFYKGVYPLGYLQVNNTSPITQEEFVMIRKVGASASEALSA
                     AKIITAAVDRLVVVDVSAGGLAVAFKDKSLLKYFKDGNYLFFTMLLPEGRSASMLVIV
                     RNISMTRNNITRVGCQIVEIDALGQAHYDEFLETMG"
     gene            4520..4978
                     /locus_tag="PKJ16_07265"
     CDS             4520..4978
                     /locus_tag="PKJ16_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46825.1"
                     /translation="MAINTTLNVHANTLRLLDDACALTGMRRGRIISMLIHRSMHDMT
                     TRIRIGRSVQYQARDHKENWHIIHVRLSEDDADYLKDLRNVLKESDSLIIAKAARLFL
                     SDIIKQATQGMRCDNYRFSNYVLSRIEEDGLIAWKIYWGYPKNPETALRL"
     gene            complement(5011..5667)
                     /locus_tag="PKJ16_07270"
     CDS             complement(5011..5667)
                     /locus_tag="PKJ16_07270"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HNV46826.1"
                     /translation="MGEVAAVFSISGVLPLSIACAAIFALREIAAYRGSLGFKYLLTP
                     LITLLIIGIAVLSAGKGLTGYQFLIVGALTLSLIGDAMLMIEEVNLFLHGLVFFLAAH
                     IAYVFAFSMGYAWQPWNLAPALVLGVLYIAYNAWVRKTQVAVYSAILMLMVFFAVSSF
                     NAGATTRAVVLCVAAVLFALSDGLLGYNTFVKRVPNSTVYTWALYAPAQFLFALSCYL
                     "
     gene            complement(5667..5945)
                     /gene="csrA"
                     /locus_tag="PKJ16_07275"
     CDS             complement(5667..5945)
                     /gene="csrA"
                     /locus_tag="PKJ16_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HNV46827.1"
                     /translation="MLVLARKVNESIMIGDDVELIVIDIKGDQVKLGIKAPKKVTVHR
                     KEIYDEIQQENIAAVSSEFKPEALRELSDFFEREKKERDGNDTKGRKA"
     gene            complement(5948..6400)
                     /gene="fliW"
                     /locus_tag="PKJ16_07280"
     CDS             complement(5948..6400)
                     /gene="fliW"
                     /locus_tag="PKJ16_07280"
                     /inference="COORDINATES: protein motif:HMM:NF009793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HNV46828.1"
                     /translation="MKVKTKPFGEIEIQDKQIVEFPEGILGFEYIKKFIIMDSSEKSP
                     FKWLQAFDEPDLAFVIIRPADFMIEYELVVAQGDLEAVGCASADDLLVFAIVTIPDNP
                     SDMTANLQGPIIINPVTHIGRQAISLSDRYRVRHRILDEVKRATEGRG"
     gene            complement(6568..7830)
                     /locus_tag="PKJ16_07285"
     CDS             complement(6568..7830)
                     /locus_tag="PKJ16_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HNV46829.1"
                     /translation="MQRVTNQMLNNTMIHNLNRHQSSMDQMQNQLGTGKSVNLPRDNP
                     IATTSQMLYRTRLTEIDQYNRNIDEAKSRMDEVDTALQSAMRIFQRLRVLAVQGAHGI
                     YTAFERKEAAAAEINELLEEVVAIANTRSATGRPIFGGFQTGSEDVQNPFVAIYQTIT
                     AGNQGDAMTGVEYRGNIGKMKREVAQGEFMDVNAPGNWVFWATNQILTSNKDASEYAA
                     DSNQKIRIDGKEINISAGDNLEIIIDKINNAGLSIRASKGGRNNLIMETTTPHQIWLE
                     DVESGRVLKDLGLINTEFPEPPNNVDPTVTIGGMSIFEMIIQLRDDLVRGDQELVGGR
                     DIGLLDLALDNILKHISAVGAKQQRTDELVKRTAFDKENVLTMLSKSEGIDFAETIMN
                     FKWLESVHQYALAVGAKTIRPTLMDFLR"
     gene            complement(7834..9738)
                     /gene="flgK"
                     /locus_tag="PKJ16_07290"
     CDS             complement(7834..9738)
                     /gene="flgK"
                     /locus_tag="PKJ16_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HNV46830.1"
                     /translation="MNSTFMGLEIGKRGIQSHQQALHVTGHNISNAENKEYSRQRVVI
                     TSAEPIYAPALNRANTPGSMGQGSVVSVVERMRDHFIDDRIVTEKDVMGYWKSKNDFI
                     RQIEGVYNEPSDLSLRSRLDQLWQSWQELSKFPQERSAREVVKEKAVNLSNDMRHVYR
                     QLYDLQLDANRKIQYTVNQINMYARDIRDLNERILKTEAVGDKPNDLMDRRDALIEKL
                     STLVDISVGRSDKDELMVYIGGENLIQGEVLRPLEAVMDPDNKGYFKVVWATTKTDPT
                     LRGGELTGLLEVRDKILRDNINDINALAINMMDLTNEVHRDGFGKTGKTNENFFKHIM
                     ISDSVEGNHDLNNDGVADVTAIFKVSGTNKLDGSAAIGVSGTLVFITNDQYEQRAEID
                     YNANDTTLGVIKKINDARLGVVAYLNHNNQLALKATIAKDTDRKNFMIRYLEDSGQFL
                     VGLTGILKNAGAQGSFDYRRVDDIRKFLPDREHVTVAPQYNPASWMAVDDSIMYDVDK
                     IAAAQGKDVGGTGDFNTTNGPGDGTNALRIANLRHKHAMVDAKTTFNEFYNALVSRIG
                     TQGEEAGDRIKNQETLLTNLMNLRESVSGVNLDEEMAQMVAFQHGYNAAARMISTFDR
                     MLETIIKMGA"
     gene            complement(9738..10244)
                     /locus_tag="PKJ16_07295"
     CDS             complement(9738..10244)
                     /locus_tag="PKJ16_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HNV46831.1"
                     /translation="MSMAVRELERVLKNEVAIYHQLYDCEEEKGRAIIARDGALIERL
                     SREQEELMRRLGALERERLALIDEYVRENRINELPGNVTLRQIVAQMDEDSSHRLLAI
                     GMALRSVLTKIQSLQETNRTLLDDNIRFFSILLSTLKDTVSVKTGYSGSGVEESRVSQ
                     SLIFNRRF"
     gene            10700..14791
                     /locus_tag="PKJ16_07300"
     CDS             10700..14791
                     /locus_tag="PKJ16_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV46832.1"
                     /translation="MISSLRLTEPIPIACTSSTPDILHDPVRVNDRIAHITAARSIHG
                     RIGALLVVGGEKFGLALFDEAGEYRTYRANTSCAAGTGSFLDQQARRLNLAGIEEFAA
                     LAQSNTGQIPRIASRCAVFAKTDLTHAQQEGYSLPEICDGLSHGMAKNICDTVFSNID
                     IPAPLLLAGGVARNGAVARHIGALAGIVPVVDDRAHLYGAMGAALTALESARTGPRLP
                     SANARDIIRAERETERAYHYPALDCALTRYPDFASWRRFEYAPRRIPGAAPIEIDIYR
                     PLAAGERLRVFLGIDIGSTSTKAAVVLADREMLAGLYTRTAGRPLEAVQAIFEALETI
                     GDEHGVRFDVAGAGTTGSGRKFIGGIIGADLVLDEISAHARAACELDPAVDTIIEIGG
                     QDAKFTTLRDGTVTFSVMNTVCAAGTGSFIEEQAAKLGCALADFARRAAGQCAPLSSD
                     RCTVFMERDLNHYLAEGFAVDEVLASVLHSVRDNYLLKVAVEGAIGRRIFFQGATAKN
                     RALVAAFEQRLGKPITVSPYCHLTGAYGAALTLIDSGAAETTFRGIGLHREEIPVRSE
                     ACDLCANHCKLKCAEVAGETVAYGFLCGRDYATNAFVSRNASGFDLIKERATAFARKK
                     RPSRHAATVGIPAALYLAEELPLWKMFFDELGIPTVTSEHYRDALKTGKRHTGAEFCA
                     PIAAFHGHVMHLADKADYLFVPTYLEARNSAGDGRRQYCYYTQFMPPLIASIKGFDPA
                     RIVSPLVNSIHGALHQKVQLYKAVKTITGGATGFLEVSAAYDAAAAFHAQRLAQLRET
                     FTREIADAGDISVVLLGRPYTVLSKHMNNNIPGIFAANGVKAFFQDMLPVEGAAPAAM
                     RPLLETFHWKYAASVLQSAEYVARTDGVYPVLVTSFKCSPDSFTVEHFKDIMGGRGKP
                     YLVLQLDEHDSSVGYETRIEAAIRSFRNHHAARRMPLPSAAALLPSNITDERSVLDGR
                     TLLIPGWDPVNCDLLAAVLTSEGIDARVLEETPDSIRRSLRHNTGQCIPLTAVAQNCI
                     DYVERHGLDPARTALWMFNANIACNVRLFPSYIKRILDAHGMERLSVYVGDMTFIDLS
                     VKTAVNAYFANLFGGMIRKISCGMRPHEVDPGDTDRAAVDALVFFRNALSRATSKERA
                     VREAVARFAEVRVRRTPKPKVAIFGDVYTRYNDVMNQGLIRTIEDNGGEVVITPFSEY
                     MMMVADPYIYKWFHEGCYSDAAIARLLKTVIPALEERYHRIFREVLDEPARRHRIDRD
                     AILRQFVMDPLHTGESMENLYKIFTLAEAHPDLSLFVQANPAFCCPSLVTQAMSDRIE
                     SLTGIPVVTIEYDGIGGFKNESVIPYLRFPRERRVPDALRAAE"
     gene            14932..15576
                     /locus_tag="PKJ16_07305"
     CDS             14932..15576
                     /locus_tag="PKJ16_07305"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV46833.1"
                     /translation="MQKLALIIIAIVVSLVAGCSKTAGIDATIIALGGEVTLNGAKPS
                     LGSKIAFGDAIETGEKSFCEIVVKDKNVFRMGASTRLVFRVSADSNILQLDRGWFAGI
                     TRKLFTKQQRYLVKTPTVTAGVRGTSYCIKVEKPESTYFCVCNGTIDLAENNAQPGEL
                     VTAAHHMGRRFSREKDGRIAIDKNPGMLYHDDVGLEALAKKIDETIDWTKPDEK"
BASE COUNT         2948 a         5136 c         4627 g         3003 t
ORIGIN      
        1 atcgtcgccg aagcggaaat cgatcgccgg cgcgatgatc tcccgccggc cgccgaacag
       61 gctcctggcc agcgacggat acgagatcac atagggacag aagacgcgcg gcttcccgtt
      121 gtcctgtcgc gtttcgctca tcaccgacgg gaagaatacg tattcggcgt cgtcgcgttc
      181 ggcgcagcgg ccgaaacggg cgagggccac cttcacgggg tagcaataat cggcgtcggt
      241 tacctgcgcg gccgcctcct tgagctcagc gccgcccccg ccctcgacga ccgtctcgaa
      301 accgagctca tgaagaaagg tccgccacag gggcagatag gtatgcatcg agagcgcctc
      361 ggggatcgcc gcgaccggcc gtcgtgtttc aacgacgcgc gcgccgccgt tcgccgcgtc
      421 gcaggacgcg cccaggcggg cgacccgcgc aaaatggtcc gtccgacgcg cggcgggcgc
      481 gggatcatcc gcctccttgc cgcaccgata tccccatgac gccacggcgg agccttcaac
      541 ccgcgcgacg gttatgcggc agtaattggc gcaccgatcg cacgtctcgt ggctggtcgc
      601 gatctcgcgc tcgaagacgc cacgcccgcg aaaggtcccc acacccgcgc ccgtccgatc
      661 gcccgcgatg agcgccgctc cgaaggcccc catcacgtga cagtagggcg acaccacgat
      721 ctccttgccg gtgagcgact cgaaagcggc gacgagcccc cggtttcgcg cggtggcccc
      781 ctggaaaaat atgcgctcgc cgctcacggg acgcgcgccg accacgcgat tgagatagtt
      841 cttcgcgatg gagcggatca cccccgccat cgcctcctcg cgcgaatagc cattgcgcag
      901 gagttgggcg atgtcctgct ccatgaacac cgtgcagcgg tcggaggtgt taggcgtgga
      961 caggcccagc accaactccc ccagcgtacg aacgtcgaag cccaggcgct cggcctgctc
     1021 ctcgatgaag gagcccgtgc cggcggcgca cacgtaattc atggcggcgt cgatcaccga
     1081 gccgttgtgc ccgcggatat atttcgcgtc ctgcccgccg atctcgaata tcgtttcgac
     1141 atcgggctga aagtgcgtcg cgccccggaa gtgcgcggtg atctcgttga cgatgacctc
     1201 ggcgccgatg atctcgccga tgagccgccg tcccgacccc gttgtcccgg cggccacgat
     1261 gcgcaggtcc gtccccgcga gcgccgactc gagcgcccga aagaggcgcc gcgccgcatc
     1321 gatgggatcg gcgccggtct tccgatagat gtcgacgagc accgtgcgcg tggacgcgtc
     1381 aaggagcgca cacttcgtgc tcgtcgagcc gatgtcgatg cccaacagcg ccgatccccc
     1441 gtcaaccacg ggggcgtgca tcctgatttc gtttccgtcg tccgcgtatt cgcgaatcgc
     1501 cgtcgatgcg gcgggccggg agcgcgcaag cgacagcgac cccgcgtacc gcgcgccctc
     1561 agcgcgcgcc acgccgcgtg cgcccaccgg tcggtgtcgc ccgccaccat cgcgaagctg
     1621 tatcgccgcg cccagcgcgg ccagaaaatg cccctcgtca atgaatcgcg cgccctcgag
     1681 gagctcttcc gtgaaacgcc gcaccgccgg attgaggaac agcccgccgc acatgagcac
     1741 cgcctcgtcg tcgaggcgcg gcgcggcgtg cagcatcgtc atcaccacgc cccggcacag
     1801 tcccgaccag agggccgcgc gcgagtgccc ctcctgctgc cggtggatga gatcggattt
     1861 cgcgaacacc gcgcaccgcg tcgcgatctc gggcgggtcg ggaacgaacg gaagcgcgcc
     1921 gatatcatca tacgaaaacc ccatgcgctt catctgctgg tcgagaaacg atccggtgcc
     1981 ggccgcgcac aggctgttct cggcgtacga ggcgaaacgc ccctctccgt ccagcgtgat
     2041 cagcttcacc gacgcggcgc cgaccgcgag caccgcatgc gcacgcgcct cccggtgccg
     2101 cagcgcggcg atgatgcacg acacctcatc aacctgcacc gcgcgcggga agcgttccgc
     2161 gatgtgcccg ccgaaaagcc ccgtgcacgc gatggccggc gcattcggga tctccgcaag
     2221 aagcgtggag gcggctccag cgggatcacc atgatgctca cggtaatcga tatgccgcac
     2281 tgtattgtcc gaaaccgacg tgaccttaat aaacttgctg cccgcatcaa tccccagaat
     2341 catggtagcc tcacaatggg tggttatggt gataggggga ggttaagcgg aaactgtcaa
     2401 tgaaaaatcg aactatttgt aaatatttgt ttttattgca acatatttga tgatattatt
     2461 ttgtattatt gaatttattg gggttgacca tgaaatcagc gacccgttat aaagccgcta
     2521 ggtaaaccta tgaaaaaagc gatagttatc tcattttgtg gcatcggatg cggcatgatg
     2581 atcgcccacg cagccataac ccggctcccc tttaatgcag ccgcactgcc gaaagggatc
     2641 gtctgcacgg gcagggtcgt cgatggagcg cgctggacgg actcgaccgg cgagaacatc
     2701 gtcgtcctct gccagaccgg cgcgttcaaa tctcctaagg gcgacagcga ggattcccgc
     2761 gacgccgagc tctccgcgca ccagtacgtc cgctccgccc cggcgtggag gcgggtgtgg
     2821 tcgattcgtg acttcgagcg aaactgcccg ctggacctca cggcgacctt tattcccggc
     2881 gcgcttaccg tcaccgacct tgacgggaac ggcattgccg aggtgaccgt tctctacaag
     2941 cacgcctgcc gcggcgacgt gtcgccggcg gggatgaagc tcatcatgcg cgagggagcg
     3001 aagaagtacg ccatccgcgg tatcatgcgg atctcgcccg agatcgaccc gagcggccgc
     3061 ggggagatga atctggaccc gacgttcgcg aaggccccgc ggtcgttcag ggatttcgcg
     3121 atacaacact ggaagaagca tgaaatcgag aaatcgttcg agcagccgtg accggcgcat
     3181 tgaagggggc cgccctgcac cgatgcggac ggcgcatgcg ctctcgaagc gctaccccat
     3241 agtctccagg aactcatcgt agtgcgcctg gcccagcgcg tcgatctcga cgatctggca
     3301 gcccacccgc gtgatgttgt tgcgcgtcat gctaatattc cgcacgatga cgagcattga
     3361 cgcggatcgt ccctccggca agagcattgt aaagaacaga taattcccgt ccttaaaata
     3421 cttcaggagg gatttatcct tgaaggcgac ggcgagcccg cccgcggaga catccacaac
     3481 cacgagccga tcgaccgccg cggtgatgat ctttgcagcg gacagcgcct cggaggccga
     3541 cgcgcccacc ttgcggatca tcacgaactc ctcctgggtg atgggcgagg tattgttcac
     3601 ctgcagatat cccaacgggt atacgccctt atagaacagc ggcaccgacg cctcggaaac
     3661 cgtggtccgc gtattgagcc ccttgtcttt ctggaagatg gtgatcttgt aaaactgata
     3721 gagcgttttc cccgcctcag ttgcgggagt ttcgcgaagg ttttcgatat acagcggcgt
     3781 ccgttcgcgg tagaaaaatt tcatccgctc gtcggcaaat cgctcgtgca aaaaacacac
     3841 gcgcgcgaac gaatagcggt cggtgaactt tccccgaatg gcgtccttga aatattcgac
     3901 ccaatccctt cctgccgtaa aacagtcctt gagcagcgta tcggacacga gattggtgat
     3961 gatgatggcg ggccgctcgc ccgcggacgg cgatgcgagt gatgtgcgct cttcgcgacg
     4021 cgcagccgta atcacctgaa ccatctgaag cgaaaacgga tactcgaacc tgttgtcgac
     4081 aaccacggcc atgctcggat cgaacgttgg cgtgatccgc gcgcaataca gcttgtcgtc
     4141 gagccgggcg aagaggacgc acgggtccgc ggtgctcaag cgctccaggg tccggagcgt
     4201 caccgatccg tcgcgatact ccttcatctc cacctgctgg ttcacatcgt ccatgcgaag
     4261 gaatagatca cggttcgtaa agattgtgtc gatgattcgc tcgatctcgt ctctgtcggt
     4321 gatgcatgta tcgttcgagc tcatgtttga ttttccgcct tttcctctct cacatattga
     4381 ttctatgggg aatcatggac ggtgtcaaaa caattttacc gctatttgaa acatattccc
     4441 ttctcagacc catgaaaaat attcactggt ttgccttcaa actcgtaatt ttattagaca
     4501 ctaaaaaatg gaggcggata tggccataaa caccacactc aatgtgcatg cgaatacact
     4561 ccgattgctc gacgacgcct gtgccctgac cggcatgcgg cgggggcgga ttatcagcat
     4621 gctcattcac cggagcatgc acgacatgac cacgcgcata cggataggac gctcggtgca
     4681 atatcaggcc cgcgaccata aggagaactg gcatataatc cacgtccgcc tcagcgagga
     4741 cgacgcggat tacttaaagg atttacggaa tgtattgaag gaatcggatt ctttgattat
     4801 tgcgaaggcg gcccggcttt tcttgtccga tataatcaaa caggcgacac aagggatgag
     4861 atgcgataac tatcgctttt caaactatgt gctctcccgt attgaggaag atggtctgat
     4921 cgcctggaag atctactggg gataccccaa aaaccccgag actgccctcc gcctctagag
     4981 gcgcaactac gcagcgaagc cgagacgatc ttacagatag caggaaaggg cgaagagaaa
     5041 ctgcgccggc gcgtagagcg cccaggtgta taccgtagaa ttggggacgc gcttcacgaa
     5101 ggtattgtac cccagcagcc cgtccgagag cgcgaagagg acggcggcga cacacaggac
     5161 gacggcgcgg gtcgtcgcgc cggcgttgaa ggacgaaacc gcgaaaaaca ccatcagcat
     5221 gagaatcgcg ctatacacgg ccacctgcgt tttacggacc caggcgttgt acgcaatata
     5281 caacacgccg aggacgaggg ccggagcgag gttccagggc tgccaggcgt atcccatcga
     5341 aaaggcgaac acgtacgcga tgtgcgccgc caagaaaaaa acgagcccgt gcaaaaagag
     5401 gttcacctct tcgatcatca gcatggcgtc gccgatgagc gaaagcgtca gggcgcccac
     5461 aatcaggaac tgatagcccg taagcccttt ccccgccgac agcacggcga tcccgattat
     5521 cagcagggtg atgagcggcg tcagcagata cttgaagccg agcgacccgc gatacgcggc
     5581 gatctcccgc aacgcgaata ttgcggcgca ggcaatcgag agcggcagaa cgccggaaat
     5641 ggaaaaaacc gccgcaacct ctcccatcag gccttcctgc cttttgtatc gttgccatcg
     5701 cgctccttct tttcccgctc gaagaagtcg gacagctcgc gcagcgcctc gggcttgaac
     5761 tcggagctca cggcggcgat attctcctgc tggatctcgt catagatctc cttgcggtgg
     5821 acggtcactt tcttgggcgc cttgatcccc agcttcacct ggtcgccctt gatgtcgatc
     5881 acgatgagct cgacatcgtc gccgatcatg atgctctcgt tcaccttccg cgcaagcacc
     5941 agcatgccta gcccctcccc tcggtcgcgc gcttcacctc gtccagaatg cggtggcgca
     6001 cgcggtatcg gtcgctcagc gagatggcct gccgaccgat gtgtgtcacg ggattgatga
     6061 tgatgggacc ctgcagattg gccgtcatgt ccgagggatt gtcggggatg gtcacgattg
     6121 cgaacaccag gagatcgtcg gcgctcgcgc agcccaccgc ctccagatcg ccctgcgcca
     6181 ccacgagctc gtattcaatc atgaaatccg ccgggcggat aatcacgaac gccaggtccg
     6241 gctcgtcgaa ggcctggagc catttaaagg gactcttctc ggaggagtcc atgatgataa
     6301 atttcttgat atactcgaat ccgaggatcc cctcggggaa ctccacgatc tgcttgtcct
     6361 ggatctcgat ctcgccaaac ggcttcgtct tcactttcac tgacaatcgg aaactcctgt
     6421 cgttattgtt tcgacgaaaa atactcgcaa cacctcaccc cgcctccggc gcgccctctc
     6481 cctcaggaga gggctctgag acgagaaccg ttccgtattg taacaccccc ttctcctacg
     6541 ggagaagggg gccgggggat gaggtggcta tcgcaggaag tccatcagcg tgggccggat
     6601 ggtcttcgcg cccaccgcca gcgcatactg gtgcaccgac tcgagccact tgaaattcat
     6661 gatggtctcc gcgaaatcga tcccctcgga cttcgacagc atcgtcaata cgttttcctt
     6721 gtcgaaggcg gtccgcttca ccagctcgtc ggtccgctgc tgctttgcgc caaccgccga
     6781 gatgtgcttg aggatattgt ccagcgccag gtccaggagg ccgatgtcgc gcccgcccac
     6841 aagctcctgg tcgccgcgca ccaggtcgtc gcgcagctgg atgatcatct cgaaaatcga
     6901 catccccccg atggtgaccg tgggatcgac attgttgggc ggctccggaa actccgtgtt
     6961 gataagcccc aggtccttga gcacccggcc gctctccacg tcctcgagcc agatctggtg
     7021 cggcgtggtg gtctccatga tcaggttgtt gcggccgccc ttggacgccc gaatgcttaa
     7081 cccggcattg ttgatcttgt caatgataat ttcgaggttg tcgccggccg agatgttgat
     7141 ctccttgccg tcgatgcgga tcttctggtt cgaatcggcc gcgtactccg acgcgtcctt
     7201 gttcgaggtg aggatctggt tggtggccca gaagacccag ttgccgggcg cgttcacgtc
     7261 catgaactcg ccctgcgcca cctcgcgctt catcttgccg atgttgccgc ggtactccac
     7321 gccggtcatc gcgtcgccct ggttcccggc ggtgatggtc tgatagatcg cgacgaacgg
     7381 gttctgcacg tcctcggagc cggtctggaa gccgccaaag atcgggcggc ccgtcgcgct
     7441 gcgcgtgttc gcgatggcca cgacctcctc gaggagctcg ttgatctccg ccgccgccgc
     7501 ctccttccgc tcgaaggcgg tgtagatgcc gtgcgcgccc tgcaccgcga gcacccgcag
     7561 gcgctggaag atgcgcatgg ccgactggag cgcggtgtcc acctcgtcca tgcgcgactt
     7621 cgcctcgtcg atgttgcggt tgtactggtc gatctccgtg agccgcgtgc ggtagagcat
     7681 ctggctcgtg gtggcgatcg gattgtcgcg cggcaggttc acgctcttgc cggtcccgag
     7741 ctggttctgc atctggtcca tgctggactg gtgccggttc aggttatgga tcatcgtatt
     7801 gttgagcatc tggttggtta cgcgttgcat ggcttaggcc cccattttga tgatggtttc
     7861 cagcatccga tcgaaagtcg agatcatccg cgcggcggca ttgtacccgt gctgaaaggc
     7921 gaccatctgc gccatctcct cgtccaggtt tacgcccgac acggactcgc gaaggttcat
     7981 gaggttcgtc agcagcgtct cctggttctt gatgcggtcg cccgcctcct ccccctgcgt
     8041 gccgatgcgc gaaacgagcg cattgtaaaa ctcgttgaag gtcgtcttcg cgtccaccat
     8101 cgcgtgcttg tgccgcaggt tcgcgatgcg cagcgcgttg gtcccgtcgc cggggccgtt
     8161 ggtggtgttg aaatcgcccg tgccgcccac gtccttgccc tgcgcggccg cgatcttgtc
     8221 aacgtcgtac atgatgctgt cgtccacggc catccacgag gcgggattgt actgcggcgc
     8281 cacggtgacg tgctcgcggt cgggcaggaa cttccggata tcgtccacgc gacggtagtc
     8341 gaatgatccc tgcgccccgg cgttcttgag gataccggtg agccccacga ggaactgccc
     8401 gctgtcctcg agatagcgga tcatgaagtt cttgcggtcc gtgtccttgg cgatggtcgc
     8461 cttgagcgcc agctggttgt tgtggttcag ataggccacc acccccaggc gcgcgtcgtt
     8521 gatcttcttg atcacgccca gcgtcgtgtc gttcgcgttg tagtcgatct ccgcgcgctg
     8581 ctcgtactgg tcgttggtga tgaacacgag cgtcccgctc acgccgatcg ccgccgagcc
     8641 gtcgagcttg ttggtcccgc tcaccttgaa gatggccgtc acgtccgcca cgccgtcgtt
     8701 gttcaggtcg tggttgccct ccacgctgtc gctgatcatg atgtgcttga agaagttctc
     8761 gttggtcttg cccgtcttcc cgaagccgtc gcggtgcacc tcgttggtca ggtccatcat
     8821 gttgatcgcg agcgcgttga tgtcgttgat gttgtcgcgc aggatcttgt cgcgcacctc
     8881 caggaggccg gtgagctccc cgccgcgcag cgtgggatcg gtcttcgttg tggcccacac
     8941 gaccttgaag taccccttgt tgtcggggtc catcaccgcc tcgagcgggc gcagcacctc
     9001 gccctggatc aggttctcgc cgccgatgta gaccatgagc tcgtccttgt cgctgcgccc
     9061 cacggagatg tccaccagcg tggagagctt ctcgatgagc gcgtcgcgcc ggtccatgag
     9121 gtcgttcggc ttgtcgccca cggcctcggt tttcaggatg cgctcgttca ggtcgcggat
     9181 gtcgcgcgcg tacatgttga tctggttcac ggtgtactgg atcttccggt tggcgtccag
     9241 ctggagatcg tagagctgcc ggtaaacgtg ccgcatgtca ttggagaggt tcaccgcctt
     9301 ctccttgacc acctcgcgcg cggagcgctc ctgcgggaat ttcgaaagct cctgccacga
     9361 ttgccacagc tggtccaggc gcgagcgaag cgacaggtcc gacggctcgt tgtacacgcc
     9421 ctcgatctgg cggatgaaat cgttcttcga tttccagtag cccatcacgt ccttctcggt
     9481 cacgatccgg tcgtcgatga agtggtcgcg catgcgctcc acgacggaga ccacgctccc
     9541 ctgtcccatg ctgcccggcg tgttcgcgcg attgagcgcc ggcgcgtaga tgggctcggc
     9601 ggaggtgatc accacgcgct gccgcgagta ctccttgttc tcggcgtttg atatgttgtg
     9661 gccggtcacg tgcagggcct gctgatggga ctggatgccc cgcttgccga tctcgagacc
     9721 catgaaggtg gaattcatca gaacctccta ttgaaaatga gggattgcga cacgcgcgac
     9781 tcctcgacgc cggagccgct gtagccggtc ttcaccgaga ccgtgtcctt gagggtggac
     9841 aacaggatgc tgaaaaagcg aatattatcg tcaaggagcg tgcggttggt ttcctgcagc
     9901 gattggatct tggtgagcac cgatcgcaac gccatcccga tcgccagcag ccggtgcgac
     9961 gagtcctcgt ccatctgggc gacgatctgc cgcagcgtca cgttcccggg cagttcgttg
    10021 atgcggtttt cgcgcacgta ctcgtcgatg agcgcgagcc gctcacgctc cagcgcgccc
    10081 agccgccgca tgagctcctc ctgctccctc gagagccgct cgatcagcgc cccgtcgcgc
    10141 gcgatgatgg cccgtccctt ctcctcctcg caatcgtaca gctggtgata gatcgcgacc
    10201 tcgttcttca gtacccgctc aagttccctc acagccatgc tcatgctccc tccctggaat
    10261 tgggtgacgg acacccgatg tgcatagtgc atcctatgat tatgtatcgg aaaaataatt
    10321 attctgataa ggattttatt cccatcgaac cgagcatttt ataaacaaat cagcacaagt
    10381 gttgatacat gtgacggcat catcaacact ctgcttcttt atgatatctt tttgcagcat
    10441 tcatgttctc ttgatatggc ccccgtttga taagcaaatg gcgccagagg cactcattgc
    10501 atttatcgct tgacataaca ttgttattct tgtaatgagc gcgtgacgaa tcgaggagaa
    10561 cattatgcgc gataatatgg tcctaggtat tgatattggt tcagtttcgg cggcaatcgt
    10621 catagtcgat tccacatcga ccgttcgggc gagcgattac cgatttcatt acggaaaacc
    10681 ccgcgaggtc ctgcgcgaca tgatttcctc gctccgcctc acggaaccga taccaatcgc
    10741 ctgcacctca tcgacccccg acatcctgca tgatcccgtt cgggtgaacg accgcatcgc
    10801 gcatatcacc gccgcgcggt cgatccacgg gcgaatcggc gcgctcctcg tggtgggcgg
    10861 cgagaagttc ggcctcgcgc tcttcgacga ggcgggcgaa taccgcacct accgcgccaa
    10921 cacctcgtgc gcggcgggga ccgggagttt tctcgaccag caggcccgcc ggctcaacct
    10981 cgccgggatc gaggagttcg ccgcgctcgc gcagtccaat acggggcaga ttccgcgcat
    11041 cgcctcgcgc tgcgccgtgt tcgccaagac cgacctcacg cacgcccagc aggagggcta
    11101 ttcgctcccg gagatctgcg acggcctctc gcacggcatg gccaagaaca tttgcgatac
    11161 ggtgttttcg aacatcgaca tccccgcgcc gcttctgctc gccggcgggg ttgcgcgcaa
    11221 cggcgccgtc gcgcggcaca tcggcgcgct cgccggcatc gtgccggtgg tggacgatcg
    11281 cgcgcacctc tacggcgcga tgggcgcggc gctcaccgcg ctcgaatcgg cgcgaacggg
    11341 cccgcgcctg ccctccgcga acgcgcgcga catcatccgt gccgaacgcg agacggagcg
    11401 tgcctaccat taccccgcgc tcgattgcgc cctcacgcgc tatcccgact tcgcctcgtg
    11461 gcgacgcttc gaatacgccc cgcgccgcat ccccggagca gcgcccatcg agatcgacat
    11521 ctaccggccg cttgcggcgg gcgaacgcct ccgcgtgttt ttgggcatcg acatcgggtc
    11581 caccagcacc aaggcggccg tcgtcctggc cgaccgtgaa atgctcgccg gcctctatac
    11641 gcggacggcc ggccgccccc tcgaggcggt gcaggccatc ttcgaggcgc tcgagaccat
    11701 cggcgacgag cacggcgtac ggttcgatgt cgccggtgcg gggaccaccg gctcgggcag
    11761 gaagttcatc ggcggcatca tcggcgccga cctggtgctc gacgagatct ccgcgcacgc
    11821 gcgcgcggcc tgcgagctcg accccgcggt ggacaccatc atcgagatcg gcgggcagga
    11881 cgcgaagttc accacgctgc gcgacggcac ggtcaccttc tcggtgatga acaccgtatg
    11941 cgccgccgga accgggagct tcatcgagga gcaggccgcg aagctcggct gcgcgctcgc
    12001 ggatttcgcc cgccgggccg cggggcaatg cgccccgctc tcgagcgacc gctgcaccgt
    12061 gttcatggag cgcgacctca accactacct cgccgagggc ttcgcggtgg acgaggtgct
    12121 cgcctcggtg ctccactccg tgcgcgacaa ctacctgctc aaggtggccg tggagggagc
    12181 catcggccgg cgcatcttct tccagggcgc caccgcgaag aaccgcgccc tggtcgccgc
    12241 cttcgagcag cgcctcggaa agcccatcac ggtgtcgccc tattgccatc tcaccggcgc
    12301 ctatggcgcg gccctgacgc tcatcgacag cggcgccgcg gagacgacgt tccgcgggat
    12361 cggcctgcac cgggaggaga tccccgtacg ctccgaggcg tgcgatctgt gcgccaacca
    12421 ctgcaagctc aagtgcgccg aggtggccgg cgagaccgtc gcctacggct tcctctgcgg
    12481 gcgcgattat gcgacgaacg ccttcgtgtc gcgcaacgca tcggggttcg atctcatcaa
    12541 ggagcgcgca acggccttcg cccggaaaaa gcgcccctcc cgccacgccg ccacggtcgg
    12601 gatcccggcc gcgctctacc tcgccgagga gctgccgctg tggaaaatgt tcttcgacga
    12661 gctgggcatt cccacggtca ccagcgagca ctatcgcgac gccctcaaga ccggaaagcg
    12721 ccacaccggc gccgagttct gcgcgcccat cgcggccttt cacggccacg tgatgcacct
    12781 cgccgacaag gccgactacc tcttcgtgcc gacatacctc gaggcgcgaa acagcgcggg
    12841 cgacggccgc cggcagtatt gctactacac ccagttcatg ccgccgctaa tcgcctccat
    12901 caagggcttc gatcccgcgc gcatcgtttc gccgctggtc aactccatcc acggcgcgct
    12961 gcaccagaag gtgcagctct acaaggccgt gaagaccatc accggcggcg caacgggttt
    13021 tctcgaggtc tcggcggcct acgacgcggc ggcggccttt cacgcacagc gcctcgcgca
    13081 acttcgcgag acgttcaccc gcgagatcgc ggacgccggc gacatctccg tggtcctgct
    13141 ggggcgtccc tacaccgtcc tctcgaaaca tatgaacaac aacatccccg gcatcttcgc
    13201 cgcgaacggc gtcaaggcct tcttccagga catgctcccc gtcgagggcg ccgcccccgc
    13261 cgcgatgcga ccgctcctcg aaaccttcca ctggaagtac gccgcgagcg ttttgcagtc
    13321 cgccgagtac gtcgcccgca ccgacggcgt ctacccggtc ctggtcacct cgttcaagtg
    13381 ctcgcccgac tcgttcaccg tcgagcactt caaggacatc atgggcgggc gcggcaaacc
    13441 gtaccttgtc ctccagctcg acgagcacga ttcgagcgtg ggctacgaga cgcgcatcga
    13501 ggccgccata cgctcgttcc ggaatcacca tgccgcacgc cgtatgccgt tgccgtccgc
    13561 ggcggcactg ctcccctcca acatcaccga cgaacggagc gtcctcgacg gaaggacgct
    13621 cctcatcccc ggctgggacc ccgtcaactg cgacctcctc gccgccgtcc tcacaagcga
    13681 gggcatcgat gcgcgcgtcc tcgaggaaac gcccgactcc atccggcgca gcctccgcca
    13741 caacaccggc cagtgcattc cgctcacggc cgtggcgcaa aactgcatcg actacgtgga
    13801 gcgccacggg ctcgacccgg cgcgcaccgc gctgtggatg ttcaacgcga acatcgcgtg
    13861 caatgtccgc ctctttccgt cgtatatcaa gcgaatcctg gacgcgcacg gcatggagcg
    13921 gctttcggtg tacgtggggg acatgacctt catcgacctg tccgtgaaga cggccgtgaa
    13981 cgcctatttc gcgaacctct tcggggggat gatccgcaag atctcgtgcg gcatgcgccc
    14041 ccacgaggtc gaccccggcg acaccgaccg cgccgccgtc gatgcgctcg tcttcttccg
    14101 caacgcgctc tcccgcgcaa catcgaaaga gcgcgccgtg cgcgaggccg tcgcccgctt
    14161 cgccgaggtg cgcgtacgcc gcacgcccaa accgaaggtc gcgatcttcg gcgacgtgta
    14221 cacgcgctac aacgacgtga tgaaccaggg cctcatccgc accatcgagg acaacggcgg
    14281 cgaggtggtc atcaccccct tcagcgaata catgatgatg gtcgccgatc cctacatcta
    14341 caagtggttc cacgagggct gttactccga cgcggcgatc gcgcgactgc tcaagacggt
    14401 cattccggcg ctcgaggagc gctatcaccg catcttccgg gaggtgctcg acgagccggc
    14461 gcgtcgccat cgcatcgacc gcgacgcgat cctccggcaa ttcgtcatgg acccgctgca
    14521 caccggcgaa tcgatggaga acctgtacaa gatattcacc ctcgccgagg cgcatcccga
    14581 cctgtcgctc ttcgtgcagg ccaacccggc cttctgctgc ccgtcgctgg tcacgcaggc
    14641 gatgagcgac cggatcgaat cgctcaccgg catcccggtc gtcaccatcg aatacgacgg
    14701 catcggagga ttcaagaacg agagcgtgat cccgtacctc cgctttccga gggagcggcg
    14761 cgtccccgat gcgctgcgcg cggcggagta ggtgcgcatc gcgatcatca tcaaagtcgg
    14821 gaacgacttc cttcaaccgc agcgatggat tcgtcatcgc aaatacggtt gacaatcgat
    14881 accgcgactg ctacgatttc gcacatcaca cagagccaat ggaggtttcc cgtgcagaaa
    14941 ctcgcgctca tcatcattgc catcgtcgtg tcgctggtcg ccggttgttc caaaaccgcc
    15001 ggcattgacg caaccatcat cgcgctggga ggggaggtta cgctcaacgg agcgaaaccg
    15061 tcacttggat cgaagatcgc cttcggggac gccatagaaa ccggcgagaa gtccttttgc
    15121 gagatcgtcg tgaaagacaa aaacgttttc cgcatgggag cgtccacccg actggtgttt
    15181 cgcgtttcgg ccgacagcaa catcctgcag ctcgatcgcg ggtggttcgc cggcatcacg
    15241 cgaaagctgt tcaccaagca acagagatac ctcgtgaaaa ccccgaccgt gaccgcgggc
    15301 gttcgcggga cctcgtactg cataaaagtc gaaaagcccg agagcaccta cttctgcgtc
    15361 tgcaacggaa ccatcgacct ggccgaaaac aacgcacaac ccggcgagct ggtcacggca
    15421 gcgcaccaca tggggcgccg cttctcccgc gagaaggacg gtcgcatcgc catcgataaa
    15481 aatcccggca tgctctacca cgacgacgtc ggactcgagg cgctcgcgaa aaaaatcgac
    15541 gagacgatcg actggacaaa accggacgag aagtaagatc gcgttccgcc gccctacgac
    15601 caatacacgg tagccggttt cgctatcgct caacgcaaac gccgcgctaa aattcagcgc
    15661 ggcgcttcgg ctacctcagg tattgggcga aagggggggg gaaggggtgt ttgc
//
LOCUS       DAORHF010000063        15695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2838_length_15695_cov_5.236381, whole genome shotgun sequence.
ACCESSION   DAORHF010000063 DAORHF010000000
VERSION     DAORHF010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15695
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2838_length_15695_cov_5.236381"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PKJ16_07310"
     CDS             complement(<1..312)
                     /locus_tag="PKJ16_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46834.1"
                     /translation="MTAPGTVLFYAAGGGLGHLARTFAIAAALPTPSLPARILCTSDL
                     AHLARDAAPAPLDRPDDAVLASRTRYWRYVTDYLDEHDVRMIVLDAFPWGIVGEWAVL
                     GR"
     gene            complement(331..933)
                     /locus_tag="PKJ16_07315"
     CDS             complement(331..933)
                     /locus_tag="PKJ16_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46835.1"
                     /translation="MNTHAGKLAVAAILALCLCAGGCSKKPEEAGGQKSLRLSSGDTL
                     AFGAAASGWEASGFGVTYAITKRSDGYQFASPAGTLTARTKDSKTKLYGPDGALRLKI
                     KRDDDKIKVLRAEEDPQAWSIRLKGGGSAYKVKRGEKTLGKAKFYPDQKHIKAKDDTG
                     AVLGRMSADSPSAAPAIAFMSELRGEDRLALFALFMLLGW"
     gene            complement(914..1981)
                     /locus_tag="PKJ16_07320"
     CDS             complement(914..1981)
                     /locus_tag="PKJ16_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46836.1"
                     /translation="MNLRAAIGEHPGLIARLGNLPVDPLADTPVLFAKIKLLWQCNLS
                     CVFCRRPRARAPMPAERALSLLTSLRARGLRKVHFSGGEVFLHPEIISILSSSAALGL
                     QVNLTTNGTLIGREEARAIVDAGAHSVSVSLDSHDPKRHDELRGHKGAFKKTSKAIEL
                     LLRYRKKDPVLRVNTVVTRKNFPDLEAMRAFIDSLGGRVEWKLIPVDSSNRSLLLDTA
                     QARALIDVAGVWTKHEAPSARAAAPTPRDITRGRYSGGYYGEHPCYMPWLHLFVDPAG
                     FAYPCCMTRGKTPALGNLADAPLDDLLGGERMKRVRMSMAAGNAFAVCRNCDDFIEEN
                     ALIESEINSLKGGIQHEHTRR"
     gene            complement(1978..3096)
                     /locus_tag="PKJ16_07325"
     CDS             complement(1978..3096)
                     /locus_tag="PKJ16_07325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV46837.1"
                     /translation="MRVLWLTTRHAPQTGGMAQSSSRLVNSLRDRAHRVAVLHLAPDV
                     PADATRAESLIRCDAADLREPERIFWKHRDAIADSLLVGFGGTIAGYLAALWARWAGA
                     KSLVLFRGNDFDRALHNTKAAWMTHFILQHATIVGAVTGEMTERIRTIRTGPVIFTPN
                     SADASQWLPLERDLAAARRFRMERGLDGVPVVAMFGELKAKKGVDLACRIFDQLGLAE
                     RARLLTVGSLPEPLAALQADPARAYWIHVPFTAHDELPAWYAASDIVFIPSLYDGMPN
                     VLLEAMAAGRIVAGSRAGGMPDVIIDGENGFLFDIADADDAARVINMILDMDDASKRA
                     IAESARRTVCERFTAEREIAAIESALCEFADEPAGARS"
     gene            complement(3099..3497)
                     /locus_tag="PKJ16_07330"
     CDS             complement(3099..3497)
                     /locus_tag="PKJ16_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46838.1"
                     /translation="MFKCKYKVGLLNLSDCNRPADETCVHCNRNVCEEHREERDEGPV
                     CLECLAEKYPKERLSEHGMQTRWRNRIFDQYGYHPHFYGAGRGVRGAGYRERDYETFD
                     EDQVPKDDDALIDEDIADEGDDTAADFQDS"
     gene            complement(3509..4345)
                     /locus_tag="PKJ16_07335"
     CDS             complement(3509..4345)
                     /locus_tag="PKJ16_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46839.1"
                     /translation="MTTGTKPPGIGGIIKKRRLAGAWNYDDLAAVLKRLESIDAHAEK
                     LRVVMIVLFILTLICAVCLTLIGLIILAIPAAVLAIVFLMLFFRYRRRDLDNGFREFL
                     APLLKGLKDDIKSGAPIAVDMDLAPLEDKTHLTETSPKYQKGAYHTCYDRRYRRDTLS
                     LGIRLSDGNTLRLSRTDLLLSCERTKRSSSGKSKTKHKQALRLVYRVQMRLNPARYRF
                     TGAGKNFEKPVTFTKTPKGHILKTSFSQKLSSTSARADPLFILEQLVKLYSNLKPAAE
                     KE"
     gene            complement(4269..5531)
                     /locus_tag="PKJ16_07340"
     CDS             complement(4269..5531)
                     /locus_tag="PKJ16_07340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV46840.1"
                     /translation="MNALFATFDVFPSPKGAGTHIAQTALALAERFDRVHLVTLGHAD
                     MPAYQREGAITLHRCLAGHPNLLRRSDYFGDHVHSVVSRLDIAPRYVHFRDIWSGLPL
                     LTHERLAGATFVFEVNGLPSVELPCHYPALAKNASLITSIRAMEDYCLAHAHGIIAVC
                     ETGRRYLVSRGVPAGSITVLPNMAADPGPDAPSPDDPRRPAGDYILYAGTAAPWQGLP
                     LLLRALRLIRSECRATLVLAISTRKHLKAVRRLVRRMGLADRVRVHVGLSRERLFALY
                     RDARFSLAPLSRCDRNELQGCSPIKILESMACGTPVIAARLPVCAEIVTHGRDGYLFT
                     PDSARSLANAMRALLGDPGLCARLGSAAEDTIRTRHSAAAWSARLLDACESYMNPHRE
                     EAYHDDGNETARHRRDHQETAACRRLEL"
     gene            5735..8065
                     /locus_tag="PKJ16_07345"
     CDS             5735..8065
                     /locus_tag="PKJ16_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNV46841.1"
                     /translation="MPQSILITQCLQNDFVKPIEKYDPLPNALHVGYDESRRLLGEIV
                     EEGPVCALMRWAYAASPDDLTIVNIRDWHDADDHAQDRHLASFGRHCVRDTEGADFVF
                     APVIVPGRASHTVNATGLNDFAGTNLAEILEPYRGRPVRVGLAGVWTEAKITFLAYEL
                     VTRFDTFRVGVSSALTASSSREMHFIALEQLANILGVEVFASPAGFVEFLAGAAPALT
                     VASHPRIDRGRIAVEGGAALGDTDRDLLLYLFRSSGELSVKCLDGGFSGNVVVKTQSR
                     DLHAHRQVPCVIKIGPRDLIAKERMAFERIQDILGNNAPAIVDFCESGDRGAIKYRYA
                     SMLDGAVRTLQELYAEADAAWVRHILEVVFVRQLGRLYEAAYRDRLDLLRYYDYSSRY
                     AAGVRRSVEAVTGRAEPDDTISLGGATVPNPCRFYERDINALAEPGSSARFVSYLHGD
                     LNGRNIIVDEQENVWLIDFFHTHRGHVLRDLIKLENDLLYIWTPVRSDDELDEAFRLS
                     DLLIGVEDLAAPLPEARFSGAQFQKAWETARVLRSFYPALIRTDRDPYQLHAGLLRYA
                     VHTLSFDEPDLRQKRWALYSAGVLTGRIRDHLTRSRRLRIDYLALPDGERIRVGMTIM
                     PGRRDWGRDLAEDLDAMREEGVSRVLALVTQDELVRYGVPGLIAEYGRRGIECRWLPV
                     LDQGVPTIAAMRDAVDWIEGCVNAGEAVLIHCVGGLGRTGAAAACYLARHRGLSAAEA
                     VAAVRRSRSGRAVESREQSAFIERFAREAHSSRSTG"
     gene            complement(8041..9300)
                     /locus_tag="PKJ16_07350"
     CDS             complement(8041..9300)
                     /locus_tag="PKJ16_07350"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HNV46842.1"
                     /translation="MQTKPAAPSFGIITAILLMAGALAPAVRCGMPAAAPAPLPTGTR
                     QAVLVVPRAPGSHTATLSLVELEGGVWRERMAPVPAVLGRAGIAPAGAKREGDGKTPS
                     GVFPLGIAFGYGATCDTRLPYRMAGPDDYWIDDPASPHYNRWVRGPRPAFSHERLRRS
                     DAQYTYGIVVEYNTAPVTPGAGSAIFLHVWRAPNTPTAGCVALSETDMARLLRLLDPS
                     LRPVVAIGAPPAQPAPPTFVPAPHLVEISGPEYVLDLRYNTDNNFLGRNVYAPHGITR
                     CYLHRDAAARLTAAARDLHAKGFRIVLWDCYRPLSVQRAMWRIMPNERYVANPAKGSN
                     HNRGIAVDCSLARADGRELPMPTPFDDFSERAAVNARCSSDAAERCRNRDLLRGAMER
                     AGLEPYPSEWWHYQLPDARRYPVLRDE"
     gene            9453..9989
                     /locus_tag="PKJ16_07355"
     CDS             9453..9989
                     /locus_tag="PKJ16_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46843.1"
                     /translation="MPLYTARFRSVKIDIVQSLQTAKGPSASEKPEMKFDVDGAMRHE
                     LVMRAKDFVDPGAWLMGLFFSALGAGFALVVIYGILYLAGAVNLTGAAWGGAKIAYLS
                     SALVLFVSCWAGSSVFLAIRRARTALIERDRGEGNWKLVDEEKWERYTTMLRLAAERV
                     EREREAAKAKAARGVVLR"
     gene            9986..10960
                     /locus_tag="PKJ16_07360"
     CDS             9986..10960
                     /locus_tag="PKJ16_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV46844.1"
                     /translation="MTAVDIPEFLRQVYVFSALGADERATIAERFRVVPVAAGEVIFR
                     EGDPGGELYIVERGRVATFINVPGGAQKEIAAFAPGDFFGEMSIFEKAPRSATCIATE
                     ESVLVSLSQEEFMSLVDPYPEIATRIMHRMLAITTQRLRTTGEFLSEIVRWGEGASKR
                     AITDEVTGVYNRRFLEEAIVSHYDTSVGTGRPLSIIMMDLDNFRGINERFGIEAADRA
                     FREVADAISRRLGDADILARYGGDEFTALLPGTPLEGAARIAERIRADVEALDVLTGL
                     TDARTCLTLSQGVVAYPESARTLKSLREKADAALYRAKENGRNRVERG"
     gene            11115..11846
                     /locus_tag="PKJ16_07365"
     CDS             11115..11846
                     /locus_tag="PKJ16_07365"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HNV46845.1"
                     /translation="MDVRALLPLGLVPLTVVAVFFLHWLTVRHVRSAAERLPLCRGAY
                     LAFSGQLLLYGWIVLPVYSAGVSAAYLVPLGMTFSFVGDVFNLQFPSILRKTGEPLFY
                     GILSFMAAQVCYIAAIVSLVSMDDLIGRGYLVPLMAGLIVVPAVLFRFRVYHPDRPKR
                     IMRGALVYGFVLGAMAALAISAALAHGGLWWLPAAGAAFFLLSDAVMGETTVHGRHPV
                     FEYQVPWVTYLAAQGLILMGFAAML"
     gene            complement(12061..12654)
                     /locus_tag="PKJ16_07370"
     CDS             complement(12061..12654)
                     /locus_tag="PKJ16_07370"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV46846.1"
                     /translation="MALPKSKADEKFTYADYRAWPDDEHWELIGGVAYDMSPSPGTGH
                     QEIVLTIARIIAEYLDGKQCKVFVSPLDVRLPESTDAAVDEDIVTVVQPDVVVVCDPR
                     KIDTRGILGAPDLAVEVLSESTAYKDETQKLLLYERHGVREYWIVNPGAGHLNLFRIG
                     QDGRYEKPLHYRANETFESIVLPGLILDCGRIFSGIE"
     gene            complement(12933..13550)
                     /locus_tag="PKJ16_07375"
     CDS             complement(12933..13550)
                     /locus_tag="PKJ16_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46847.1"
                     /translation="MADVKLNPVFEGFRRKIGDLVFFNRNGELFVRRKGTQRNPKTSE
                     QMEVRNAFTQLVNNWKGIGGILQRGWGNAAKGSDKTGYNLFIGANAKRQRSGEVLELF
                     KPVGDDTLFEFVAAPGTEPGSISVSFAPVNGGHHVTLFTQKKTNGAASGMFSRHDSGE
                     NPAGPVVLSGLEPAAEYFVYGVITDAAYPAATRVTRSHGTLCVSA"
     gene            14078..>15695
                     /locus_tag="PKJ16_07380"
     CDS             14078..>15695
                     /locus_tag="PKJ16_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HNV46848.1"
                     /translation="MLDASGWAVQDKSDINLSSQRGVAIRELSFATGEPDYTLFADGK
                     AIGVVEAKPEGTTLRGVESQSEKYATGAPPGIPAWHNPLAFCYESTGTETHFTNRLDP
                     DFRSREVFSFHRPETLIEYAQQPKQLNERIRELPPLPTGHLWPAQAQAIANLEKSFAR
                     NMPRALIQMATGSGKTFTAVNFTYRLVKFAKARRVLFLVDRGNLGDQTLKEFQQFVTP
                     DDGRKFTELYNVQHLVSNTIDPVARVTISTIQRLYSMLRGDEEPPEDLDDLAIDAAES
                     LYRDPVPVAYSPVFPPETYDFVVIDECHRSIYNLWRQVLEYFDAYLIGLTATPSKQTF
                     GFFHQNLVMEYNHERAVADGVNAAYDVYRIRTNISEKGSKIESGYYVDKRDRLTRAKR
                     WEQLDEEFEYTAKELDREVVAEDQIRTVLTAFRDRLFTEIFPGRTDVPKTLIFAKDDS
                     HAEDIVRICREVFAKGNEFCQKITYRTTGAKPKDLISSFRNSYHPRIAVTVDMIATGT
                     DIKPLEIVFFMRSVKSQGFFDQMKGRGVRVI"
BASE COUNT         2605 a         4858 c         5349 g         2883 t
ORIGIN      
        1 gcgccccaga acggcccatt cgccgacgat gccccagggg aaggcgtcca gcacgatcat
       61 tcgcacgtcg tgttcgtcga ggtagtctgt cacataacgc cagtaccgcg ttcgcgaggc
      121 gaggaccgcg tcgtcggggc ggtcgagggg cgctggcgcc gcgtcccggg cgaggtgcgc
      181 gaggtcgctc gtgcacagga tgcgcgccgg gagcgagggc gtcgggaggg ccgcggcgat
      241 cgcgaacgtc cgcgcgagat gtcccaggcc cccgcccgct gcgtagaaga gcacggtgcc
      301 gggcgcggtc atgaaatcgc gccgccgggc ctaccagcca aggagcatga acagggcgaa
      361 gagcgcgagc cggtcctcgc cccggagttc gctcatgaag gcgatcgcgg gcgccgcgct
      421 cggcgaatcg gcggacatgc ggcccagcac cgcgcccgta tcatccttcg cctttatgtg
      481 cttctggtcg gggtagaact tcgctttgcc cagggttttc tcgccgcgct tgaccttgta
      541 cgccgatcct ccgcccttca ggcgtatgga ccacgcctgc gggtcctcct cggcgcgcag
      601 caccttgatc ttatcgtcgt cgcgcttgat cttgaggcgg agcgccccgt cgggtccgta
      661 gagcttcgtt ttcgaatcct tggtgcgcgc cgtgagcgtc ccggccgggg aggcgaattg
      721 atagccgtcg gagcgtttcg tgatcgcgta ggtcacgccg aatccgctcg cctcccagcc
      781 cgatgccgcc gcgccgaagg cgagggtgtc gccggaggag aggcggaggg atttctgccc
      841 gccggcctcc tccggttttt tcgagcagcc gccggcgcac aggcatagcg ccaggattgc
      901 cgcgacggcg agtttacctg cgtgtgtgtt catgctgaat acctcctttg agggaattga
      961 tttccgattc gatgagggcg ttctcctcga tgaagtcgtc gcagttgcga cagaccgcga
     1021 aggcgttccc ggcggccatg gacatgcgca cgcgcttcat gcgctcgccc cccaggagat
     1081 cgtcgagggg ggcgtcggcg agattgccga gggctggcgt tttcccgcgc gtcatgcagc
     1141 agggataggc gaaaccggcg gggtccacga agagatggag ccagggcatg tagcagggat
     1201 gctccccgta atagccgccg gagtagcgtc cccgcgtgat atcgcgcggc gtgggggccg
     1261 ccgcgcgcgc tgatggcgcc tcgtgcttcg tccagacgcc ggctacgtcg atgagcgccc
     1321 gggcctgcgc ggtatcgaga aggagcgacc tgttcgagga gtccacgggg atgagcttcc
     1381 attcgacgcg cccgcccagc gaatcgatga aggcgcgcat ggcctcgagg tcgggaaaat
     1441 tcttccgcgt gaccacggtg ttcacgcgca gcacggggtc ctttttgcga tagcgcagca
     1501 aaagctcgat ggccttgctc gtcttcttga aggcgccctt gtggccgcgc agttcgtcgt
     1561 gccgcttggg atcgtgggag tccagcgaca ccgaaacgga gtgggcgccg gcgtcaacga
     1621 tggcgcgcgc ctcctcgcgg ccgatgagcg tgccgttggt ggtgaggttc acctggagcc
     1681 ccagcgcggc cgacgaggac aggatcgata tgatctccgg atgaagaaat acctcgccgc
     1741 cggagaagtg aaccttgcgc aggccccgcg cgcgcagcga tgtaagcagg gacaaggcgc
     1801 gctcggcggg catgggcgcc cgggcgcgcg gacggcggca gaagacgcag gagagattgc
     1861 actgccataa cagcttgatc ttcgcgaaga ggaccggcgt gtccgcgagc gggtccacgg
     1921 gaaggtttcc gaggcgcgcg atgagccccg ggtgctcgcc gatggccgcg cgcaggttca
     1981 cgaacgcgca cccgccggtt cgtcggcgaa ttcgcacagc gccgactcga tggcggcgat
     2041 ctcgcgctcg gcggtgaatc gctcgcacac ggtgcgccgg gcgctctcgg caatggcgcg
     2101 tttcgatgcg tcgtccatgt ccaggatcat gttgatcacg cgcgcggcgt cgtcggcgtc
     2161 ggcgatatcg aacaggaagc cgttctcgcc gtcgatgatg acgtcgggca tgcccccggc
     2221 gcggctgccg gccacgatcc gccccgcggc catcgcctcg aggagcacgt tgggcatgcc
     2281 gtcgtaaagc gaggggatga acacgatgtc ggaggcggcg taccaggcgg ggagttcgtc
     2341 atgcgccgtg aagggcacgt ggatccagta ggcgcgcgcg ggatcggcct gcagggccgc
     2401 aagcggttcg gggagcgatc ccacggtgag cagccgcgcg cgctcggcca ggccgagttg
     2461 atcgaagatg cggcaagcca gatcgacgcc cttcttggcc ttgagctcgc cgaacatggc
     2521 cacgacggga acgccgtcga gtccgcgctc catgcggaag cgccgcgcgg cagcgaggtc
     2581 gcgctcgagg ggaagccact ggctcgcgtc cgcgctgttg ggcgtgaaga taacgggccc
     2641 tgtgcggatg gtgcggatgc gttcggtcat ctcgccggtg accgcgccca cgatcgttgc
     2701 gtgctgcaaa atgaagtgcg tcatccaggc ggccttggtg ttgtggagcg cccggtcgaa
     2761 gtcgttgccg cggaagagca ccaggctctt cgcgcccgcc cagcgcgccc agagcgcggc
     2821 gaggtacccg gcgatggtgc cgccgaagcc caccagaagc gaatcggcga tggcgtcgcg
     2881 gtgtttccag aagatgcgct cgggctcgcg caggtcggcg gcgtcgcagc ggatgaggct
     2941 ctcggcgcgc gtggcgtcgg cggggacgtc gggcgcgagg tggagcaccg cgacccggtg
     3001 cgcgcggtcg cggaggctgt tgaccagccg cgacgacgat tgcgccatcc cgcccgtctg
     3061 cggggcgtgg cgcgtggtga gccagagcac gcgcatggct agctgtcctg gaagtccgcg
     3121 gcggtgtcgt cgccctcgtc ggcgatatcc tcgtcgatga gagcgtcatc gtccttcggc
     3181 acctggtcct cgtcgaaggt ctcgtagtct cgctcgcggt agcccgcgcc gcgcacgccc
     3241 cgtccggccc cgtagaagtg cgggtggtaa ccgtattgat cgaagatgcg attgcgccag
     3301 cgcgtttgca tgccgtgctc gctgagccgc tctttgggat acttctccgc gaggcactcc
     3361 aggcacaccg gcccctcgtc acgctcctcg cggtgctctt cgcacacgtt ccggttgcag
     3421 tgcacgcagg tctcgtccgc ggggcggttg caatcgctca ggttaagcag tccgaccttg
     3481 tatttgcatt tgaacatggc ggcctccctc attccttttc cgcggctggt ttcaggttcg
     3541 aatagagctt cacgagctgt tccagaatga agagcggatc ggcgcgggcc gacgtgctcg
     3601 agagcttctg ggaaaaggac gttttgagga tgtgcccctt cggggtcttc gtgaaggtga
     3661 ccggtttctc gaaattcttg ccggcgcccg tgaagcggta gcgcgcggga ttgaggcgca
     3721 tctggacacg atacacgagc cgcaacgcct gtttgtgttt ggtcttcgac ttgccggagc
     3781 tgcttcgctt ggtgcgctcg cacgagagca ggaggtccgt gcgggacaga cggagggtgt
     3841 tcccgtcgga gagccggatt cccagcgaga gggtgtcgcg ccggtaccgg cgatcgtagc
     3901 aggtgtgata cgcgcccttc tgatacttgg gcgaggtctc cgtcaggtgg gtcttgtcct
     3961 caagcggggc gaggtccatg tccacggcga tgggcgcgcc ggacttgatg tcgtccttga
     4021 gccccttcag gagcggtgcg agaaactcgc gaaagccgtt gtccaggtcg cgccgccgat
     4081 agcggaagaa cagcatgagg aatacgatcg ccagcacggc cgccggtatc gcgaggatga
     4141 tgagcccgat caacgtcaga cagaccgcac agatgagcgt gaggatgaag agtacgatca
     4201 tcaccacgcg cagtttctcc gcgtgggcgt cgatgctctc cagccgtttc aggacggccg
     4261 cgaggtcgtc atagttccag gcgccggcaa gccgccgttt cttgatgatc ccgccgatgc
     4321 cgggcggttt cgttcccgtc gtcatgatat gcctcctcac ggtgtgggtt catgtatgat
     4381 tcgcacgcgt cgagcagccg cgcgctccag gcggccgcac tatgacgggt gcggatggtg
     4441 tcttctgcgg cggaccccag gcgcgcgcac agcccggggt cgccgagcag cgcgcgcatg
     4501 gcgttggcga gcgagcgcgc ggagtccggg gtgaagagat agccgtcgcg cccgtgcgtg
     4561 acgatctccg cgcagaccgg caggcgcgcc gcgatcaccg gcgttccgca ggccatggat
     4621 tccagtatct tgatgggcga gcagccctgc agctcgttgc ggtcgcaacg cgacagcggg
     4681 gcgagcgaga agcgcgcgtc ccggtagagc gcgaagaggc gctcgcgcga caggcccacg
     4741 tgtacgcgca cgcggtccgc cagccccatg cggcgcacca ggcggcggac cgccttcagg
     4801 tgcttgcgcg tcgagatcgc gagcaccagc gttgcgcgac actccgaccg gatgaggcgc
     4861 agggcgcgca gcaggagcgg gagcccctgc cagggggcgg ccgtgccggc gtacaggatg
     4921 tagtcgccgg cgggacggcg cgggtcgtcg ggcgacggcg cgtccggtcc ggggtccgcg
     4981 gccatgttgg gcagcaccgt gatggatccg gcggggacgc cgcgcgagac caggtagcgg
     5041 cggccggtct cgcacacggc gatgatcccg tgcgcgtgcg ccaggcagta gtcctccatg
     5101 gcgcggatgg acgtgatcag cgatgcgttt ttcgccagcg ccgggtagtg gcagggaagc
     5161 tccacggagg gcaagccgtt cacctcgaat acgaaggtcg ctcccgcgag gcgctcgtgc
     5221 gtcaatagcg gcagcccgct ccagatgtcg cggaaatgga cgtagcgggg cgcgatgtcc
     5281 aggcgcgaga ccaccgaatg cacgtggtcg ccgaagtagt cgctgcggcg caggagattg
     5341 gggtgccccg ccaggcagcg gtgcagggtg atggcgccct cgcgctggta ggccggcatg
     5401 tcggcatggc ccaacgtgac caggtgcacc cgatcgaagc gctcggcgag cgccagcgcc
     5461 gtctgggcga tgtgggtccc cgcccccttc ggggagggaa acacgtcgaa tgttgcgaaa
     5521 agagcgttca tggtgcgtcc ggggcgcgct tccactggcg agggtaccct acgatattgc
     5581 gcgaaaaaat caacaataat tatccccgtg ccgtgggtcg ttctgcggat gcgatgacgc
     5641 cgcgcggtat ttgtgttgat acatatgccg cgctgttgta ttcttccacg gggccgcgcg
     5701 ccgcggcccg atcataccgc acacgggagg agccatgccg cagtcgatcc tcatcaccca
     5761 gtgcctgcag aacgacttcg tgaagcccat cgagaagtac gatcccctgc ccaacgccct
     5821 gcacgtgggc tacgacgagt cgcgccgcct gctgggcgag atcgtcgagg aggggccggt
     5881 gtgcgcgctc atgcgctggg cctatgccgc atccccggac gacctgacga tcgtgaatat
     5941 ccgcgactgg cacgacgccg acgatcatgc gcaggaccgt cacctcgcgt cgttcgggcg
     6001 ccactgcgtg cgcgacaccg agggcgcgga cttcgtcttc gcgccggtga tcgtccccgg
     6061 gcgcgcgagc cacaccgtga acgccaccgg cctcaacgac ttcgcgggca cgaacctggc
     6121 ggagatcctg gagccgtacc gggggcgacc ggtgcgcgtt ggcctggcgg gcgtgtggac
     6181 cgaggccaag atcaccttcc tggcctatga gctcgtcacg cgcttcgaca ccttccgcgt
     6241 gggcgtctca agcgcgctca ccgcgagctc gtcgcgcgag atgcacttca tcgcgctcga
     6301 gcagctcgcg aacatcctgg gggttgaggt gttcgcctcg ccggcgggct tcgtcgagtt
     6361 tctcgcgggc gcggcgccgg cgcttaccgt cgcctcgcac ccgcggatcg accgcgggcg
     6421 catcgccgtg gagggcgggg cggcgctcgg cgacaccgac cgcgacctgc tcctgtatct
     6481 cttccgctcg tcgggcgagc tctcggtgaa gtgcctggac ggcgggtttt cgggcaacgt
     6541 ggtcgtgaaa acgcagagcc gcgacctgca cgcccaccgg caggtcccgt gcgtgatcaa
     6601 gatcggcccg cgggacctca tcgcgaagga gcggatggcc ttcgagcgga tccaggacat
     6661 cctgggaaac aatgcgccgg ccatcgtgga cttttgcgaa tcgggcgacc gcggcgcgat
     6721 caagtaccgc tacgcctcga tgctcgacgg cgcggtgcgc accctgcagg agctttacgc
     6781 ggaggccgat gcggcgtggg tgcggcatat cctggaggtg gttttcgtcc ggcagctcgg
     6841 ccgtctgtac gaggctgcct atcgcgatcg gctggacctg ctgcgctatt acgactactc
     6901 gtcgcgctat gccgccggcg tgcgtcggag cgtggaggcc gtcacggggc gcgcggagcc
     6961 ggacgacacg atatcgctgg gcggcgcgac cgtacccaac ccgtgccgct tctacgagcg
     7021 cgacatcaac gcgctcgcgg agccgggctc gtcggcccgg ttcgtctcct atctgcacgg
     7081 cgacctcaac gggcgcaaca tcatcgtgga cgagcaggag aacgtgtggc tcatcgactt
     7141 ctttcacacg caccgggggc atgtcctgcg cgacctcatc aagctcgaaa acgacctcct
     7201 gtacatctgg acgccggtgc gctccgacga cgagctcgac gaggccttcc gtctgagcga
     7261 tctcctcatc ggcgtggagg acctcgccgc gccgctgccg gaggcgcgct tttcgggcgc
     7321 gcagttccag aaggcctggg agaccgcgcg cgtcctgcgc tcgttctatc cggcgctcat
     7381 caggaccgat cgcgatccct accagctcca cgcggggctc ctgcgctatg cggtgcatac
     7441 gctctccttc gacgaaccgg acctccggca gaagcgctgg gcgctctaca gcgcgggcgt
     7501 cctcacgggg cgcattcggg accatctcac gcgatcgcgc cgcctgcgga tcgattacct
     7561 cgcgcttccc gacggcgagc gcatacgcgt tggcatgacc atcatgcccg gccggcggga
     7621 ctgggggcgc gacctcgcgg aggacctgga cgccatgcgc gaggaaggcg tgtcgcgcgt
     7681 gctggcgctg gtaacgcagg acgagctggt gcgctacggc gtgccgggcc tgatcgccga
     7741 atacgggcgc cgcgggatcg aatgccgctg gctaccagtg ctggaccagg gcgtccccac
     7801 gattgcggcc atgcgcgatg cggtggactg gatcgagggt tgcgtgaacg caggggaggc
     7861 ggtgctgatc cactgcgtgg gcgggctggg gcggaccggc gcggccgcgg cgtgctatct
     7921 ggcgcggcac cgggggcttt ccgcagccga ggcggttgcc gcggtgcggc gcagccggtc
     7981 cgggcgcgcc gtggagtcgc gcgagcagtc cgccttcatc gagcgcttcg cgcgcgaggc
     8041 tcactcgtcg cgcagcacgg ggtagcggcg cgcgtcgggg agctgatagt gccaccactc
     8101 cgacggatag ggctcgaggc ccgcgcgctc catcgcgccg cgcaggaggt cgcggttgcg
     8161 gcagcgctcg gcggcgtcag atgaacaacg cgcgttcacg gcggcgcgct cgctgaagtc
     8221 gtcgaagggc gtgggcatgg gaagctcccg cccgtccgcg cgcgcaagcg agcagtccac
     8281 ggcgatgccg cggttgtggt tcgatccctt cgcggggttc gccacgtagc gttcgttcgg
     8341 catgatgcgc cacatcgcgc gctgcacgga aagcggccgg tagcaatccc acagcacgat
     8401 ccgaaatccc ttcgcatgca gatcccgcgc agcggcggta aggcgcgcgg cggcgtcgcg
     8461 gtgcaggtaa cagcgggtga tcccgtgcgg cgcgtatacg ttacgtccca ggaaattatt
     8521 gtccgtgttg taccggaggt ccaggacata ctccgggccg gatatctcga cgaggtgtgg
     8581 cgcggggacg aatgtcggcg gcgcgggctg cgcgggcggc gcgccgatcg cgaccaccgg
     8641 gcgcagggac gggtcgagca ggcgaaggag ccgcgccata tctgtctcgg agagggccac
     8701 gcagccggcc gtgggagtat tgggcgcacg ccacacatga aggaagatgg cgctccccgc
     8761 gccgggcgtc acgggcgcgg tgttgtactc caccacgatg ccgtatgtat actgggcgtc
     8821 gctccgtcgc aggcgctcgt gcgaaaaggc cgggcgcgga ccgcgcaccc agcggttgta
     8881 gtgcggggac gcgggatcgt cgatccagta gtcgtcggga ccggccatgc gataggggag
     8941 ccgggtgtcg caggtcgcgc cgtaaccgaa ggcgatcccc agcgggaaca ccccggaggg
     9001 cgttttcccg tcgccctcgc gcttggcccc cgcgggcgcg atccccgcgc ggcccagcac
     9061 ggccggtacg ggggccatgc gctcgcgcca cacgccgccc tcgagctcca cgagcgagag
     9121 cgtggcggta tgcgaaccgg gggcacgcgg aacaaccagc acggcctgcc gcgtccccgt
     9181 tggcaggggc gccggggcgg cggcgggcat gccgcagcgc accgcgggcg cgagcgcgcc
     9241 cgccatcaac agtattgccg tgataatccc gaacgatggt gcagccggct ttgtttgcat
     9301 gacgcgttcc ctcaattccc tcgcgttgac atcgagttac acagcacgcc taatgacagg
     9361 aaaccgcgta ttccatcaac tctaataaca attgacagac cgacatttat ggcggtaatg
     9421 ggacgcacta atcaagaaaa tgagagggcc ccatgccgct gtataccgcc cggtttcgaa
     9481 gcgtgaagat cgacattgtg caatccctgc agaccgcgaa gggcccgtcg gcctccgaga
     9541 agcccgagat gaagttcgac gtggacgggg cgatgcgcca cgagctcgtg atgcgcgcga
     9601 aggacttcgt ggacccgggc gcgtggctca tgggactctt cttctcggcg ctgggcgcgg
     9661 gctttgcgct cgtggtgatc tacggcatcc tctatttggc gggggccgtg aacctgacgg
     9721 gcgcggcctg gggcggcgct aagatcgcct acctgtcgag cgcgctggtg ctgttcgtct
     9781 cgtgctgggc gggctcgtcc gtgttcctcg cgatccgccg cgctcggacg gcgctcatcg
     9841 aacgcgatcg cggggagggg aactggaagc tggtggacga ggagaagtgg gagcgctaca
     9901 cgaccatgct gcgtctcgcc gcggagcgcg tcgagcgcga gcgtgaggcg gcgaaggcga
     9961 aagcggcccg gggcgttgtc ctgcgatgac cgcggtcgat atccccgagt tcctccggca
    10021 ggtatacgtc ttttcggccc tgggggcgga cgagcgcgcg acgatcgccg agcgctttcg
    10081 cgtcgttccc gtcgcggcgg gcgaggtcat ctttcgcgag ggcgatccgg ggggcgagct
    10141 gtatatcgtg gagcgcggcc gggtggccac ctttattaac gtgcccggcg gcgcgcaaaa
    10201 ggagatcgcg gccttcgcgc cgggggactt cttcggcgag atgagcatct tcgagaaggc
    10261 cccgcgctcg gccacctgca tcgccaccga ggagagcgta ctggtgtcgc tgtcgcagga
    10321 ggagttcatg tccctggtgg acccgtaccc ggagatcgcg acgcgcatca tgcaccggat
    10381 gctcgccatc accacgcaac gcctgcgcac caccggggag tttctttccg agatcgtccg
    10441 ctggggcgag ggggccagca aacgcgccat caccgacgag gtcaccggcg tgtacaaccg
    10501 gcgcttcctt gaggaggcca tcgtgagcca ctatgatacg tcggttggca cggggcggcc
    10561 gctctcgatc atcatgatgg acctggacaa cttccgcggg ataaacgagc gcttcggcat
    10621 cgaggcggcc gaccgggcct tccgggaggt cgccgatgcg atttcccggc ggttgggcga
    10681 tgcggacatt ctcgcgcgct acgggggcga cgagtttacc gcgctcctcc ccggcacgcc
    10741 gctcgagggc gcggcgcgca tcgccgaacg gatccgggcc gacgtggagg cgctcgacgt
    10801 ccttaccggc ctgacggacg cacggacctg cctcaccctg agccagggcg tggtggccta
    10861 ccccgaaagc gcgcgcaccc tgaagtccct ccgcgaaaag gccgacgccg cgctctatcg
    10921 cgccaaggaa aacgggcgca accgcgtgga gcgcgggtaa gacctcaacc gcttcgaccg
    10981 gctcagcgca acgcccccgt ccccttctcc gctgggagaa gggggacgag gtgatccatc
    11041 gaaaaaaaca gttgctccgc gttcttcccc ggcgtaccgt ttcccgaata tgaagatcca
    11101 cgaggaggtg tcccatggat gtccgcgcgc ttctgccgct tggcctggtc ccgctgacgg
    11161 tcgttgccgt cttttttctg cactggctca cggtgcgaca tgtaagaagc gcggccgaac
    11221 ggctgccatt gtgccggggg gcctatctgg ccttctcggg ccagttgttg ctgtatggat
    11281 ggattgtgct cccagtgtac tccgcgggcg tgtcggctgc ctatctggtc cccctcggga
    11341 tgaccttctc gttcgtgggc gatgtgttta atctgcagtt tccttctatt ttaagaaaaa
    11401 ccggcgagcc cctcttttat ggcattctga gcttcatggc ggcacaggtc tgttatatcg
    11461 ccgccatcgt gtcgctggtc tcgatggacg atcttatcgg ccgcggatat ctggtccccc
    11521 tcatggcggg gcttatcgtg gtgccggcgg tgctcttccg gttccgggtt tatcatccgg
    11581 accgccccaa gcggatcatg cgcggtgcgc tcgtgtacgg gtttgtcctg ggggcgatgg
    11641 cggcgctcgc catatcggcg gcgctggcgc acgggggcct gtggtggctg ccggctgcgg
    11701 gcgcggcctt cttccttttg tcggacgcgg tgatgggcga gacaacggtg cacgggcgtc
    11761 atccggtgtt cgagtaccag gtgccgtggg tcacgtatct tgccgctcaa gggctcattc
    11821 ttatgggctt cgcggcgatg ttataggcat ggagtgacct cgcccgcttc gacaagctca
    11881 gcgcaacgcc cgcgttcccc tttgcgacag gatgtcgcga atgcggcatc gtacacggat
    11941 gaatcccgaa ttcccccctt ctcctgcggg agaagggggc aggggggatg aggttggccg
    12001 ttgcacttcg ataggtgtgg ttttaggtaa aatcccgcga aaccccgcag caacaggctt
    12061 ttactcgatg ccgctgaaga tgcgtccgca gtcgaggatg agtccgggga gcacgatgct
    12121 ttcaaacgtt tcattggccc ggtaatggag gggtttttca tagcggccat cctgcccaat
    12181 tcgaaaaagg ttcaggtgcc ccgcccccgg gtttacgatc cagtattccc gaacgccgtg
    12241 ccgttcgtag agcagcaact tttgcgtctc gtccttgtat gcggtggatt cggaaagcac
    12301 ctccaccgcc aagtcgggag cgcccaggat tccccgcgtg tcgattttcc ggggatcgca
    12361 cacgacaacg acatcgggct ggaccacggt gacgatgtct tcgtccacgg cggcgtctgt
    12421 cgactcgggc aatcgtacgt cgaggggaga tacaaacacc ttgcattgtt tcccatcgag
    12481 atactctgcg atgatacgtg caatagttaa tacaatttcc tgatggccag tcccgggcga
    12541 tgggctcatg tcgtaggcca caccgccaat gagttcccaa tgctcgtcgt cgggccacgc
    12601 gcggtaatca gcgtaggtga atttctcatc tgcttttgat tttggcagag ccattgttgc
    12661 gcctccatgt attcatcttt tgtgtagcat gctgcggatt ttctgtcaac actttacctc
    12721 acccgcgcgc tgcgcgcccc tctcccattg gagaggggct tctcgggaga ggactttttc
    12781 tgaggagccc ctctttgcat gcagagaggg gcgcgccgac ttgccccgag cctgtcgaga
    12841 ggcggcgggg gtgagtttgg tgttcatgaa gcggatcaat acccgatttc cccccttctc
    12901 ctacgggaga aggggggtca ggggggatga ggttacgccg atacgcagag cgtgccgtgc
    12961 gagcgtgtca cgcgagtggc cgccgggtag gcggcgtcgg tgatcacgcc gtacacgaag
    13021 tactcggcgg cgggttcgag accggaaagg accacgggcc ctgcgggatt ttcaccgctg
    13081 tcgtggcgtg aaaacatgcc gctcgcggcg ccgttggttt tcttttgcgt aaagagggtc
    13141 acgtggtggc cgccgttcac cggggcgaag ctcacgctga tggagcccgg ctcggttccg
    13201 ggggccgcga caaactcgaa gagcgtgtcg tcgcccaccg gcttgaacaa ttccagcacc
    13261 tccccggagc gctgtcgttt cgcgttagcg ccgatgaaga gattgtagcc ggttttatcg
    13321 cttcctttgg cggcgttccc ccagccgcgc tgcagaatgc cgccgatccc cttccagttg
    13381 ttgaccagtt gcgtgaaggc gttgcggacc tccatctgct cggacgtttt gggattgcgc
    13441 tgcgttccct tgcggcgcac gaagagctcg ccgtttcggt tgaaaaacac cagatcgccg
    13501 attttacggc ggaaaccctc gaataccggg ttaagcttta catctgccat gagtagtacc
    13561 tccattcatt agatttggtt gatggcattg taaaaaacgt cttttccgtc cgattcgact
    13621 gttttatgtt ttcatttcgc acctcctttt cgacgtacta cttgttacac gggcgccgaa
    13681 ccggaatttt gaattttttt gatgggggcg gcttaatatt tataatttcg gggtggggaa
    13741 gcgtggtgtg agtagctaag aggtatctaa gaggtaccta agagggatgt aagaggtgtg
    13801 tggggccggg gtaaaacaat cgattcgtat ttaatttctc atcttctcct atattgagaa
    13861 ggctcctgag gggatgtcgc agtgtcgaac ggggacagag tgttcaaact ccgctcgtcg
    13921 gatgcagggg attgcggtgg gcttgtcggg aaaaaaatat tgacattaca cttttatctg
    13981 tttaccactc ttgattggct gtggaaatcg tctttgttct taatccggag gtttggtgag
    14041 cgctccagaa gaacgcgccc gtgtgcagat cgacgtgatg ctcgatgcgt cgggttgggc
    14101 ggtgcaagac aaatccgata ttaatctttc gtcacaacgg ggcgtcgcga tcagagagct
    14161 gtcgtttgcc acgggcgaac cggattacac gctctttgcg gacggcaagg ccattggggt
    14221 ggttgaggcc aagcccgaag ggaccacact gcgcggcgtg gagtcgcagt ctgagaaata
    14281 cgccaccggc gcgcctcccg gcattccggc atggcataat ccccttgcgt tttgctacga
    14341 gagcaccggc actgaaaccc atttcaccaa ccggctcgat cccgacttca ggagcagaga
    14401 ggtcttttcc tttcatcgcc cggaaacgct tattgaatac gcgcaacaac cgaagcagct
    14461 caatgaacgc attcgcgaac tgccgccgct gccaaccggg cacctctggc ccgcgcaggc
    14521 gcaggctatc gccaatttgg agaaatcatt tgcaaggaac atgccgcgcg cccttatcca
    14581 gatggcgacc ggctccggaa agaccttcac cgccgtgaat ttcacctatc gcctggtgaa
    14641 gttcgcgaag gcccgccggg tcctcttcct ggtggaccgg ggcaacctgg gagatcagac
    14701 cttaaaggag ttccagcagt tcgtgacccc cgacgacgga cgcaagttca ccgagctgta
    14761 caacgtgcag catctggtat cgaacacgat agatccggtg gcgcgcgtta ccatatccac
    14821 catccagcgc ctgtattcca tgctcagggg cgatgaggag ccgccggaag acctggacga
    14881 tctggccatc gacgcggcgg agtcgctgta ccgcgatccg gtgcccgtgg catacagccc
    14941 cgtgtttcct cccgagacat acgattttgt ggtcatcgat gagtgccatc gctccatata
    15001 taatctctgg cggcaggtgc tggaatattt cgatgcgtat ctcatcggcc tcaccgccac
    15061 gccgtccaag cagaccttcg gctttttcca tcagaacctg gtgatggagt acaaccacga
    15121 gcgcgccgtg gccgacggcg tgaacgcagc ctacgatgtg taccgcatcc gcacaaatat
    15181 ctccgagaag ggatcaaaga tcgagtccgg gtactatgtg gacaagcgcg accgactcac
    15241 ccgcgccaag cgctgggagc agctcgacga ggagttcgaa tacaccgcga aggagctcga
    15301 ccgcgaggtg gtggccgagg accagatacg aaccgtgctc acggccttcc gcgaccggct
    15361 gtttaccgag atatttccgg gccgcaccga cgtgcccaag acgctcatct tcgccaagga
    15421 cgattcccac gcagaggaca tcgtgcgcat ctgccgcgag gtgttcgcca agggaaacga
    15481 gttttgccaa aagatcacct atcgaacgac gggcgcgaag cccaaggacc tcatctcctc
    15541 gtttcgcaac agctatcatc cccgtatcgc cgtgaccgtg gacatgatcg ccacgggcac
    15601 cgacatcaag ccgcttgaga tcgtgttctt catgcgttcg gtgaagtcgc agggcttttt
    15661 cgatcagatg aaggggcggg gcgtgcgggt gatcg
//
LOCUS       DAORHF010000064        15599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2868_length_15599_cov_5.835306, whole genome shotgun sequence.
ACCESSION   DAORHF010000064 DAORHF010000000
VERSION     DAORHF010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2868_length_15599_cov_5.835306"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PKJ16_07385"
     CDS             complement(<1..642)
                     /locus_tag="PKJ16_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007292664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HNV46849.1"
                     /translation="MPIDSKKKPELSANARVVLEKRYLRKDESGAPLETPEDLFARVA
                     RYIAAADASYGRTADELARTATRFYDLMADLVFIPNSPTLMNAGRSLGQLSACFVLPV
                     EDSIDGIFDAIKNMALIHKSGGGTGFSFSRLRPHNDIVQTTTGVSSGPLSFMKIFNTA
                     TETIKQGGTRRGANMAILSVAHPDIEDFIDAKRDTAELTNFNLSVGITEEIMRA"
     gene            947..1939
                     /locus_tag="PKJ16_07390"
     CDS             947..1939
                     /locus_tag="PKJ16_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HNV46850.1"
                     /translation="MVEFKKLRIAFALLASTIVFGTTGYMVVEGMSPFDSLYQTIITI
                     STVGYQEVKPLSTAGRVVTMVLISTGITVGAYTIGTVLRLFIEGEFQRTFGRRKVEKR
                     IAGLKNHYIICGFGRIGSLVCREFRENNLPFVVIDRDPAAVRDLEGENFLFLQLDATS
                     EEALEAAGIARAKGLITAVRSDADNVFITLTAKGIRPDIFVLARASDEKSESKLKRAG
                     ATRVVSPYLIGGRRMAQVILKPTVIEFIDIAMMAKNLGLVMEEARVRDGSDLAGKNLI
                     ESNLRRDFGVIIVAIQKHGGEMIFNPTPEVRLEENDVIVVLGQKTDVTRMNKIL"
     gene            complement(1942..3813)
                     /locus_tag="PKJ16_07395"
     CDS             complement(1942..3813)
                     /locus_tag="PKJ16_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46851.1"
                     /translation="MNSESSDINTGEANASPVNAPYAVFDVLDFPVCVIGPDGSIAYR
                     NRSFARIFELDDAVRLDATHPFAPEYRRRIALAYTRVLKGQDRKCFAVVSSGGASIPI
                     ELHLYPMFHERVVTAILVFIKVVEERLLSFDRSTAESPDGDRDGDASLYDYAPFPIVR
                     MDEATGIVKYGPTFETLIGCAFEDFTRKKLSLPHFLSAFDYERIRKAVADITAGSVAF
                     RRLNEIRFTVGDEVKWTNAVLYAQRAAGPGAVEMILEDYSERKRLEDKLSFMNRLQIV
                     GDLSVGLLHSFNNIINIILSRTQLLLQLAEKESVLEGLRIIEKNANESVKQIRIIQSF
                     IDRGEQFLGEEEENIIDAIEDSIEFAKILYKVQERKRKRSIRIERRYFCVLTVKTNGQ
                     LLREVFVSMLFSLYPYIMRGDEVQLVLKDDELPELSVSVAKSAAPAETLDAATEGLFL
                     SELDIRRSADKLGIKIIEEESPAEYTIRAILPQQIIASRERARDDEGVDYKLRNLNVL
                     VVEDEKELREVISEMFDHIGNRVAVFERAEDALAELREKEYAILVSDYDLPGMTGLEL
                     MAKAKEINESIVTALLTGWSLDNIGAYKNIVDIFLTKPFKLEVLIRSISRIMKSRKK"
     gene            complement(3880..5322)
                     /locus_tag="PKJ16_07400"
     CDS             complement(3880..5322)
                     /locus_tag="PKJ16_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46852.1"
                     /translation="MKKRLTIIFVIALVIVNVACGVSYQKLMEKPEAMFYQGQFKEAA
                     RELYKEHGSVGSKDEMLILMECGLMLNAAQDYPNSTKVLMKAGKMADKINISISREAA
                     GLLLDQTMTNYKGEDFERVLIHMYLGINFLFMKKPNEARIEFKKVNDVLKDIKVSSSG
                     KTYRQNVMAMYLSAIAFEMSADLEDSEKEKFGDWQTAYVSYKQIAELEPRLPMLPTDL
                     QRLAKFIGDNEDYRKWVGRYGRRDNLPKDAGEFIMIYQAGRSAIKVSRGPLLSDQTFN
                     VGLNVALRSASLAGGLTAVAVMAALNKAENPIPKWQRRANNISHMVINVNGVDLGRTV
                     MLNNIEDTAIQTFDDTEYPALVKKTIAGMVVKLGASAAGGFAVKHLTKFILKQAGLGS
                     IADAGGNLAGVAAGAGIMTGLIASLKPDLRCWHTLPANLQLSRLFLPAGNYNIKIKFI
                     DKYGRIERFTEHQVQIGKGQKTFFNFRTLY"
     gene            complement(5387..5962)
                     /locus_tag="PKJ16_07405"
     CDS             complement(5387..5962)
                     /locus_tag="PKJ16_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46853.1"
                     /translation="MRKITIIMLVAFAAVSIVGCGGTQYLKDGDVKGSMEWGPREIKD
                     TVDKMVGSMYTFLKQEYKKPALIQVKPILNKTSEHIETNLVADELTTNLIQKRIKFID
                     RTRTKEAIKEMEDGMTGMIDPASAVPVGNLKSPNMYLYGAIRDQWVTVGSKRIQYLLV
                     TLQLNSLATSETLWQDQKKFIKATSKSEFSF"
     gene            6195..6779
                     /gene="lptE"
                     /locus_tag="PKJ16_07410"
     CDS             6195..6779
                     /gene="lptE"
                     /locus_tag="PKJ16_07410"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HNV46854.1"
                     /translation="MRTSIVGMLMALGALLAACSGGQVAGDSTTGKVLGALRGEPVIP
                     RAANTIIIPFFRDYSGEPTVSDVLTIRLRDAISMDGRLAIVADNEKADLRLVGKVMLY
                     RIQPVHYSTSGEPVRKRLMIAVIVSLVDQKNQRDIFRNRQVQSFEIFSDVVPPVVPES
                     QARDLAVESLARRIAVQTVSGWYTDLMSREERGK"
     gene            6776..7819
                     /gene="holA"
                     /locus_tag="PKJ16_07415"
     CDS             6776..7819
                     /gene="holA"
                     /locus_tag="PKJ16_07415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HNV46855.1"
                     /translation="MRAAKQALNAKQFARDIEKKPLARRYLFLGEDAGEKDRCIAGIA
                     KRLFGDAPPPPDAAARFHAEGGEFADAVGFALAPSMFSAEALCVIVNVDELSAVKENQ
                     ALMARLLDELPETVTLVLTASEKGSPKVVPAARAGEFVTVQFWRPFESDVVSHVRQRL
                     GAPRDGSAPLRIDNAAVSLLVELLGRDLARIDEALDRILAAGVRGAVTVETVREYVRD
                     AGSAGVFDLVEALFRRDRKSFRLLERLLADGVPELVVVSLVMRQAELIERFHSLTAGG
                     DAVEEALERLKVYPKHRDGFMTQARAFPPRTLARVFPLIADTDRRLKSGSMGRGLVAN
                     PLCDLTMGMVLGA"
     gene            7816..10578
                     /locus_tag="PKJ16_07420"
     CDS             7816..10578
                     /locus_tag="PKJ16_07420"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HNV46856.1"
                     /translation="MIDQRYLDKIEENRGRFCELNAIKFNRFQGLLPANIKRVINFIP
                     FMLSINHPKLPGYVEGEVPVGIVDYAPDEETRKYVKAKFPSTRVEVPEGASFIHMLAV
                     MGSVGTIAYNKKSDFDYWVCIDMRSVPADMLARFKRKVEEVQRWASAEAEVSVHLFVN
                     DITRVKQNVFAEDEDEAFGSTIGEVLKDEFFRSSIIIAGRIPFWWVLPNFLKNSDYER
                     LFAALPPDVAARYVDLGNLYKISREDFLGAALFQIIKSLGNPFKSILKLGVLEKYLFG
                     QEESPILSQKMKMNVLRGNFDNNIIDSYLLMFKEVFDYYSATLEDKKLLEVLKQNLYL
                     KVDPQISKYLKLIQDRREIPYKVEVMVRYCKDWGWDRGVISDLDNFENWEFGKTMAFW
                     DSVKKFMLLSYQRIVSRLPTLNLKDKISESDFMLLSRKIKTHFRKERDKIEQLITFKD
                     TPSEPILYIEPASQGVHELEWRLFKRNRSEENVFITTTIATEKDLLKLLAWTALNQIY
                     DPVFTRLNIQSGYTRVNQNMAVEFVNAISNFFKPGKIRIRNEYFLNEEFRLLNLIILN
                     FNLEGADAIKTVHHLYHTSWDESYLKEYEGEVNIPVILAAVLRDGVRLGRPFDDYCAV
                     IAPEIYKKAFKRVQTIFRESYAAIVERGGKGEEASARFITRIGEQYVMVTRDGAEVNA
                     QTFPSYAVLLAAKTLNPKPRMRYVFCDDEGPLAALDALYRMGREHHIVMAYEERGEAM
                     LCYVIDENGNLFSFIRPTAQRDEVLVYLYGFCRSVLKRLKASSSFSLLAEGNILVNRL
                     AFDKFGKFTVSDESINVHKRYIVGYSGERSIAVQVARHLSDETFYNVLFPDGVSSGFM
                     STGELYAVSDRIRKLRQAGHDLATCVRDITFTDLTEAEEKLGSTIYLLEKYKLESVIE
                     KGLRQM"
     gene            complement(10596..11177)
                     /locus_tag="PKJ16_07425"
     CDS             complement(10596..11177)
                     /locus_tag="PKJ16_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46857.1"
                     /translation="MKVIHALKRIETIDQDIRDLKKLEKTIKTNKSFSTPIYMSIEKQ
                     INILLGERIKLLELKIANPPAMYLEEDAAAEPAPAREPERPAPKPAVEKKAKPKKPRK
                     KEKPPVDDEEDMEEISMLTQSLIDEKIEKLQSERAGEAAPTPIAAESPRTDDESVKLL
                     DIALEKGTLGGQPADKEKKKVRFFRNNFPGGEY"
     gene            complement(11248..11973)
                     /locus_tag="PKJ16_07430"
     CDS             complement(11248..11973)
                     /locus_tag="PKJ16_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV46858.1"
                     /translation="MFFIDHAEALGGAITIVEIKGPVNAETSPDFERYLTRLLEQNKT
                     FIALDMGGVDYVSSAGIGLMLYMQKAIASRNGFLVVYRLPSEVRSLYEILGFDKVFTV
                     TDTRDEALRIIEKQNELRESPAATPAGMGADAIPSQEIDAALAEDQPTSAPVVEIAEP
                     SATNAPQARDDALSVGPPDRETDAAPPADALVNDAEVDFARSLIVECAKCRSLVRVRR
                     SGSYLCPDCHASFTVEKDQTIIF"
     gene            complement(11977..12957)
                     /locus_tag="PKJ16_07435"
     CDS             complement(11977..12957)
                     /locus_tag="PKJ16_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46859.1"
                     /translation="MTGAPRTPSFPIGMLLFMLALLLGLSAAVAGGYGYLTARARIAE
                     TEAATLRLAGVVADAAAGIAELSVATGDLARLRALFAEKAADGTVREAFYARRDGTIL
                     LHTNDALANTTIGAAPGGGPSYPADRILEPIASGKRAAAIIEYDISGAALPAIPYARH
                     LLPLVKRHLYPGIDRSGWLMVRPVVIKATPVGATVLVIGRDRVHGEFTRTVGETLRLA
                     ALLAAASCLIALCTTVVFYLRLRALAARVSAAPGPAPAATIPLAKQDPLEREIPLRDM
                     PEDGFEVREIAGEPGEHAPEGAPAGPRPETQAPVDVRRPIKNAIPVKRKR"
     gene            13186..13857
                     /locus_tag="PKJ16_07440"
     CDS             13186..13857
                     /locus_tag="PKJ16_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HNV46860.1"
                     /translation="MTPHTPEEEIEEWDWTTARPTGRPVPRRRAHREGIAHEGVHLWV
                     FRLDREDPELLFQHRAANKDMYPSCLDITVGGHVPFGTAENKIQKEAFEEIGIAPEER
                     DLVDLGYFRYEEFEPGRIHREFQRVWLYRDERALDGYRFTDGEVDAIAAVPVYAVEGM
                     LRTDMGFLAEWCAGGRIETRELSRKDFHPLLFAPSMAAYMDVVIGAVKEYARRGAVSV
                     RMPAP"
     gene            13919..15298
                     /locus_tag="PKJ16_07445"
     CDS             13919..15298
                     /locus_tag="PKJ16_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46861.1"
                     /translation="MVWARWRFHARHTPARRGAVINETILYLDVVDAHGGLKPEFLAS
                     FTSGEREVLDRINRTVADGNSLQEVIGAYFDEVRTLLPCNRLDVAFLEEGDERLVLQY
                     SIADYEPLHLQRGYASDIAGSPVEKIVHERCASIIHDMARYAEENPVSASARLLLQEG
                     IRSSMTCPLSVENRPVGLILCRSSRVNAFDERSVLLHFAVAERLSQAVEKAYRLDQLD
                     SVLQNYMEMLSFVSHEIKSPLASIITLGKTLASGYFGAIDERHRDIVTRIVQKAEYLH
                     NLSVEYLNLARFESGQMELKAYPVQFFDEVISPAIEIIAPQLFESHVEFFRDLPHEME
                     PVTCNPELMKIVLVNFLSNGIKYGRPRGTLKLTVRYLNRRVRFSVWNEGAGFPPEDRV
                     KLFKKFSTLNIPGRERIKGHGVGLYVCWKIVHLHGGHIWADSEYGAWAEFTVEIPQFG
                     DMCIIGKAD"
BASE COUNT         2699 a         4671 c         5177 g         3052 t
ORIGIN      
        1 cgcccgcata atctcttcgg taatgccaac actgagattg aagttggtga gctccgcggt
       61 gtcgcgcttc gcatcgatga agtcctcgat gtccgggtgc gcgaccgaga ggatcgccat
      121 gttggccccg cgccgcgtgc ccccctgctt gatggtctcg gtcgccgtgt tgaagatctt
      181 catgaacgac agcggcccgc tcgagacccc ggtggtggtt tgcacgatgt cgttgtgcgg
      241 gcgcaggcgc gagaaggaga atcccgtgcc gccgccgctc ttgtggatga gcgccatgtt
      301 cttgatggcg tcgaaaatgc cgtcgatgga gtcctcgacc gggaggacga agcaggccga
      361 gagctgtccc agcgagcgcc cggcgttcat gagcgtgggg ctgttgggaa tgaaaacgag
      421 atcggccatg agatcgtaga agcgggtcgc cgtgcgcgcg agctcatcgg ccgtgcggcc
      481 atagctcgcg tcggcggcgg cgatatagcg cgccacgcgg gcgaacaggt cctccggcgt
      541 ttccaacggc gcgcccgatt cgtccttccg tagatagcgc ttctcgagga ccacgcgggc
      601 gttcgcggag agttcgggtt ttttcttcga gtcgatgggc agcgagcgcc agtcgacggc
      661 gggtgcggtt gattcgcctt gtgtgggcac gaagggtttc ctcctgcatt tttcggtgcg
      721 ccccggcccg ttgcggttgc gtggttctat ggtatagacg gggaggcttg cgcgggtgta
      781 cgctacggcc aacagtagga agatgtcgcg gcgttgtcat cataaatttt caaaaaaacg
      841 cgtcgatgat gcggacggcg catgcgatca tacgggcgcc gtaaaagtat ttgacgggca
      901 accgggcgcg tgttggactg atggtgcagg tcaccggatt atccacatgg tagagttcaa
      961 aaaacttcgc atcgcttttg cgctgctggc gtccaccatc gtcttcggca cgacgggcta
     1021 catggtggtc gaggggatgt ctcccttcga cagtctctat caaaccatca tcaccatcag
     1081 cacggtgggc taccaggagg tgaaaccgct gtcaaccgcc ggccgggtgg tcactatggt
     1141 gctcatcagc acggggatca ccgtgggggc ctacaccatc ggaacggtgc tgcgcctgtt
     1201 catcgagggg gaattccagc ggaccttcgg gaggagaaaa gtggaaaagc ggatcgcggg
     1261 gctcaagaac cattacatca tctgcggatt cgggcggatc ggcagcctcg tctgccgcga
     1321 gttccgcgag aacaatcttc ccttcgtcgt catcgaccgc gaccccgcag cggtcaggga
     1381 cctcgagggg gagaactttc tgttcctgca gctcgacgcc acctccgagg aggcgctcga
     1441 ggccgccggg atcgcgcgcg cgaagggcct catcaccgcc gtccggtccg acgccgacaa
     1501 cgtgttcatc accctcaccg cgaaggggat ccggccggac atcttcgtgc tggcgcgcgc
     1561 ctccgacgag aagagcgagt cgaagctcaa gcgcgcgggg gccacgcgcg tcgtgtcgcc
     1621 ctacctcatc ggcgggcggc gcatggcgca ggtgatcctc aagcccacgg tgatcgagtt
     1681 catcgacatc gcgatgatgg ccaagaacct ggggttggtc atggaggagg cgcgggtgcg
     1741 cgacgggtcg gacctcgcgg gaaaaaacct gatcgagagc aacctgcggc gcgatttcgg
     1801 cgtgatcatc gtggcgatcc agaagcacgg cggggagatg atcttcaacc cgacgcccga
     1861 ggtccggctc gaggagaatg acgtgatcgt cgtgctgggc cagaagaccg acgtgacccg
     1921 catgaacaag atcctctgag gctatttctt gcgggacttc atgatccgcg agatgctgcg
     1981 gatgagcacc tcgagcttga agggcttggt gaggaagatg tccacgatgt tcttgtaggc
     2041 gccgatgttg tcgagcgacc atcccgtgag gagcgccgtc acgatggact cgttgatctc
     2101 cttcgccttc gccatgagct ccagccccgt catgcccggc aggtcgtagt ccgacaccag
     2161 aatggcgtat tccttttccc tgagctcggc cagcgcgtcc tccgcgcgct cgaacaccgc
     2221 cacgcggttg ccgatgtggt cgaacatctc ggagatgacc tcgcgcagct ccttttcatc
     2281 ctccaccacc agcacgttga ggttgcgcag cttgtagtcg acgccctcgt cgtcgcgcgc
     2341 gcgctcgcgg ctggcgatga tctgctgcgg gagtatcgcg cggatcgtgt attccgccgg
     2401 cgactcctcc tcgatgatct tgatgcccag cttgtcggcg gagcggcgga tgtccagctc
     2461 gctcaggaag aggccctcgg tcgcggcgtc gagcgtctcg gccggcgccg cggacttcgc
     2521 gacggacacc gagagctccg gcagctcgtc gtccttgagg accagctgga cctcgtcgcc
     2581 gcgcatgatg taggggtaga gggaaaagag catcgatacg aacacctcgc gcaggagctg
     2641 gccgttggtt ttcacggtga gcacgcagaa gtagcgacgc tcgatgcgga tgctgcgctt
     2701 gcgcttgcgc tcctgcacct tgtagaggat cttcgcgaac tcgatcgagt cctcgatggc
     2761 gtcgatgatg ttctcctcct cctcgccgag gaactgctcg ccgcggtcga tgaagctctg
     2821 gatgatcctg atctgcttca cgctctcgtt ggcgttcttc tcgatgatgc gcaggccctc
     2881 gaggacgctc tccttctcgg cgagctgcag caagagctgg gtccggctga ggatgatgtt
     2941 gatgatattg ttgaatgagt gcagcagccc cacggagagg tcgcccacga tctgcaggcg
     3001 gttcatgaac gagagcttgt cctcgagccg cttgcgctcc gagtagtcct cgaggatcat
     3061 ctccacggcc cccgggccgg cggcgcgctg ggcgtagagc acggcgttgg tccacttgac
     3121 ctcgtcgccc acggtgaagc ggatctcgtt gagccgccgg aatgcgacgc tccccgcggt
     3181 gatgtcggcg acggccttgc ggatgcgctc gtaatcgaat gcggacagga agtgcggcag
     3241 cgagagtttc ttccgggtga aatcctcgaa ggcgcagccg atgagggttt cgaaggtggg
     3301 cccgtacttg acgatgcccg tcgcctcgtc catccgcacg atgggaaacg gcgcataatc
     3361 gtacagggac gcgtctccgt cccggtcgcc gtcgggcgac tcggcggttg atcggtcgaa
     3421 cgagaggagc cgctcctcga ccaccttgat gaacaccagg atggcggtca cgacgcgctc
     3481 gtgaaacatc ggatagaggt ggagctcgat ggggatgctg gcgccgcccg aggagaccac
     3541 cgcgaagcac ttgcggtcct ggcccttgag gacgcgggta taggcgaggg cgatgcggcg
     3601 gcggtactcc ggcgcgaagg gatgcgtcgc gtcgagccgg acggcgtcgt cgagctcgaa
     3661 gatgcgcgcg aagctccggt ttcggtaggc gatcgagccg tcggggccga tcacgcacac
     3721 cgggaagtcg agcacgtcga agaccgcgta gggtgcgttc acgggagagg cgttcgcctc
     3781 tcccgtgttg atgtctgaag attcgctatt cattgggccg accgatagaa cggtgtacgg
     3841 gcgaaatccg ccgcttccac cggccggaac gaccgatgat cagtacagcg tgcggaagtt
     3901 gaaaaatgtt ttttgcccct ttccgatttg cacctgatgc tcggtaaaac gctcgatgcg
     3961 tccgtatttg tcaatgaatt taattttgat gttgtagttg cccgccggga gaaacagccg
     4021 cgaaagctgc agattcgccg gcagggtgtg ccagcagcgc aggtcgggct tgagcgaggc
     4081 gatgagcccc gtcatgatgc ccgcgccggc cgccacgccc gcgaggttcc cgcccgcgtc
     4141 ggcgatgctg ccgagtcccg cctgcttgag aatgaacttg gtgaggtgct tcacggcgaa
     4201 gccgccggcc gcactggccc caagcttcac gaccatcccc gcgatggtct ttttgaccag
     4261 ggccggatac tccgtgtcgt cgaaggtctg gatggccgtg tcctcgatat tgttgagcat
     4321 cacggtgcgt cccaggtcga cgccgttgac gttaatgacc atgtggctga tgttgttcgc
     4381 ccgtcgctgc cacttcggga tggggttttc cgccttgttg agcgcggcca tcaccgccac
     4441 cgcggtgagt ccgccggcga gcgaggcgct gcgaagcgcg acattgaggc cgacattgaa
     4501 ggtctggtcc gaaagcagcg gtccgcgcga aaccttgatg gcgctgcgcc cggcctggta
     4561 gatcatgatg aactccccgg cgtccttggg caggttgtcg cgccgaccgt agcggccaac
     4621 ccacttgcga tagtcttcgt tgtcgccgat gaacttggcg agacgctgca ggtccgtggg
     4681 cagcatgggc aggcgcggct cgagctcggc gatctgttta tagctcacgt acgccgtttg
     4741 ccagtcgccg aacttctcct tctcggagtc ctcgaggtcg gccgacatct cgaaggcgat
     4801 cgcgctcagg tacatggcca tgacgttctg ccggtaggtc ttcccgctgc tcgagacctt
     4861 tatgtccttg agcacgtcgt tgaccttctt gaattcgatg cgcgcctcgt tcggcttctt
     4921 catgaacagg aagttgatgc ccaggtacat gtggatcagg acgcgctcga agtcctcgcc
     4981 cttgtagttg gtcatggtct ggtcgagaag gaggcccgcc gcctcgcgac tgatgctgat
     5041 gttgatcttg tcggccatct tcccggcctt catgaggacc ttggtgctgt tcggatagtc
     5101 ctgcgcggcg ttgagcatga ggccgcactc catcagaatg agcatttcgt ccttgctgcc
     5161 gacggacccg tgctccttgt agagctcgcg ggcggcttcc ttgaactgcc cctggtagaa
     5221 catcgcctcg ggcttttcca tcagcttctg atagctgacg ccgcaggcga cgttgacgat
     5281 gaccaaggcg attacgaata tgatggttaa tcgttttttc atatgggata tccagtgtat
     5341 ggggttgatc ccgcccggct ctttcgccgg gcgggtcgtt gaatgcttag aacgagaact
     5401 cggacttgct cgtcgccttg atgaatttct tctggtcctg ccagagtgtc tcgctggtgg
     5461 ccaggctgtt gagctgcagg gtcacgagga gatactggat gcgcttgctg cccaccgtga
     5521 cccactggtc gcgaatggcg ccgtagaggt acatgttggg cgacttgagg ttccccaccg
     5581 ggaccgcgga ggcgggatcg atcatcccgg tcatgccgtc ctccatctcc ttgatggctt
     5641 ccttggtgcg cgtgcggtcg atgaacttga tgcgcttctg gatgaggttc gtcgtgagct
     5701 cgtcggcgac gaggttggtc tcgatgtgct cggaggtctt gttgaggatg ggcttcacct
     5761 ggatgagcgc cggctttttg tattcctgct tgaggaaggt gtacatcgag ccgaccatct
     5821 tgtcgacggt gtccttgatc tcgcgggggc cccattccat cgagcccttc acgtcgccgt
     5881 ccttgaggta ttgcgtgccg ccgcagccga cgatggaaac ggcagcgaac gccaccagca
     5941 ttataatcgt gatctttcgc atgggaaaac gctccttctt tgatttttgt gattccgatt
     6001 gatggtatgt gcgtaccaac tatgagggcg gtttgtgcgc cgtgtcaacc atgatacggg
     6061 gcgttttttg aaaatttcgg gcgcgcacgg acgaggcggt atggcacaaa aaaattattt
     6121 tgaccatgcc ggcgcgcgag cagcacgcgt attttttact tgaattattt gtcgtccccg
     6181 atgcaagacg tggtatgcga acgtcgattg tcggtatgct gatggcgctg ggcgcgctcc
     6241 tggcggcctg ttccgggggc caggtcgccg gcgactccac gaccggaaag gtcctggggg
     6301 cgctccgcgg tgagccggtg atcccgcgcg cggcgaacac catcatcatc ccgttcttca
     6361 gggattactc gggcgaaccg acggtgtccg acgtgctcac catccgcctg cgcgacgcga
     6421 tcagcatgga cggccgcctg gcgatcgtcg cggacaacga aaaggcggat ctgcgcctgg
     6481 tgggcaaggt gatgctctac cgcatacagc cggtgcacta cagcacctcg ggagagccgg
     6541 tgcgcaagcg gctcatgatc gccgtgatcg tgtcgctggt cgaccagaaa aaccagcggg
     6601 acatcttccg caaccgccag gtgcagtcct tcgagatatt ctccgacgtg gtgccccccg
     6661 tggtgccgga gagccaggcg cgcgacctgg ccgtggagag cctcgcgcgc cgcatcgcgg
     6721 tgcagaccgt atcgggctgg tacacggatc tcatgtcgcg cgaggagcga ggaaaatgag
     6781 ggccgccaaa caggcgctga atgccaagca gttcgcgcgg gacatcgaga agaagccgct
     6841 cgcgcgacgc tacctcttcc tgggcgagga cgcgggcgag aaggaccgct gcatcgccgg
     6901 tatcgcgaag cggctcttcg gcgacgcccc gcccccgccg gacgccgcgg cgcgctttca
     6961 cgccgagggc ggggagttcg ccgacgccgt ggggttcgcg cttgcgccct cgatgttttc
     7021 cgcggaggcg ctgtgcgtga tcgtgaacgt tgacgagctc tccgcggtca aggagaacca
     7081 ggcgctcatg gcccggctcc tcgacgagct gccggagacg gtcacgctgg tgctcaccgc
     7141 ctcggagaag ggatcgccca aggtcgtgcc cgccgcgcgc gcgggcgaat tcgtcacggt
     7201 gcagttctgg cgtcccttcg agagcgacgt cgtctcccat gtgcgccagc ggctcggtgc
     7261 gccgcgcgac ggctcggcgc cgcttagaat cgacaacgcg gcggtgtcgc ttctcgtcga
     7321 gcttctgggt cgcgacctgg cgcggatcga cgaggcgctg gaccgcatcc tcgccgccgg
     7381 cgtgcgcggc gcggtgacgg tcgagacggt gcgggaatac gtgcgcgacg cggggagcgc
     7441 gggcgtgttc gatctggtcg aggcgctctt ccggcgggac cggaaatcct tccgcctgct
     7501 ggagcgcctg ctcgccgacg gcgtgcccga gctcgtggtc gtgagcctgg tgatgcggca
     7561 ggcggagctc atcgagcgct tccattccct gaccgccggc ggcgacgccg tcgaggaggc
     7621 cctcgagcgc ctcaaggtct atccgaagca ccgcgacggt ttcatgacgc aggcgcgcgc
     7681 cttcccgccg cggacgctcg cgcgggtgtt cccgctcatc gccgacaccg accggcggct
     7741 caagagcggc agcatgggcc gcgggctcgt cgcgaacccg ctgtgcgatc ttacgatggg
     7801 catggtgctg ggcgcatgat cgatcaacgc tatctcgaca agatcgaaga gaaccggggg
     7861 cggttttgtg agctcaatgc gatcaagttc aaccgcttcc aggggctgct tcccgcgaac
     7921 atcaagcggg tcatcaattt cattcccttc atgctctcca tcaaccatcc caagctgccc
     7981 ggatatgtcg agggcgaggt gcccgtgggc atcgtcgatt atgcgcccga cgaggagacg
     8041 cgcaagtacg tgaaggcgaa atttccctcc acgcgcgtgg aggtccccga gggcgcgtcg
     8101 ttcattcaca tgctggcggt catgggcagc gtgggcacca tcgcctacaa caagaagtcc
     8161 gacttcgact actgggtgtg catcgacatg cgctccgtgc cggcggacat gctcgcgcgc
     8221 ttcaagcgca aggtggagga ggtccagcgc tgggcctcgg ccgaggcgga ggtctcggtg
     8281 cacctgttcg tcaacgacat cacgcgcgtg aagcagaacg tgttcgcgga ggacgaggac
     8341 gaggcgttcg gttccaccat cggggaggtg cttaaggacg agttctttcg cagctccatc
     8401 atcatcgctg gccgtatccc gttctggtgg gtgctgccga attttttgaa gaacagcgac
     8461 tacgagcgcc tcttcgcggc tcttcctccc gacgtggcgg cgcggtacgt ggacctgggg
     8521 aacctctaca agatatcccg cgaggacttc ctgggcgcgg cgctctttca aatcatcaag
     8581 tcgctgggca atcccttcaa gtcgatcctg aagctcgggg tgctggagaa atacctcttc
     8641 ggccaggagg agtcgcccat cctgagccag aagatgaaga tgaacgtgct gcgcgggaac
     8701 ttcgacaaca acatcatcga ctcctatctt ctcatgttca aggaggtgtt cgattactac
     8761 agcgcgacgc tcgaggacaa aaagctcctc gaggtgctca agcagaacct gtacctcaag
     8821 gtggacccgc agatctcgaa gtacctgaag ctcatccagg accggcggga gatcccgtac
     8881 aaggtggagg tgatggtccg ctactgcaag gactggggct gggaccgcgg cgtgattagc
     8941 gacctggaca acttcgagaa ctgggagttc ggcaagacta tggccttctg ggattcggtg
     9001 aagaagttca tgctgctgtc gtatcagcgg atcgtctcgc gcctgcccac cctgaacctg
     9061 aaggacaaga tctccgagtc ggacttcatg ctcttgagcc gcaagatcaa gacgcatttc
     9121 cgcaaggagc gcgacaagat cgagcagctc atcaccttca aggacacgcc cagcgagccc
     9181 atcctgtaca tcgagccggc gagccagggc gtgcacgagc tggagtggcg gctcttcaag
     9241 cgcaaccgga gcgaggagaa cgtcttcatc accaccacca tcgccaccga gaaggacctc
     9301 ctcaagctgt tggcctggac ggcgctcaac cagatctacg atccggtgtt cacgcggctc
     9361 aatatccagt cgggctacac gcgcgtgaac cagaacatgg cggttgagtt cgtgaacgcc
     9421 atctcgaact ttttcaagcc cgggaagatc cggatccgca acgagtactt cctgaacgag
     9481 gagttccggc tgctcaatct catcatcctc aacttcaacc tggagggcgc cgacgccatc
     9541 aagacggtgc accacctcta tcacacgagc tgggacgaat cgtacctgaa ggagtacgag
     9601 ggcgaggtga acatcccggt gatcctcgcc gccgtgctgc gggacggcgt gcgcctgggc
     9661 cggcccttcg acgactactg cgcggtgatc gcgcccgaga tttacaagaa ggccttcaag
     9721 cgcgtgcaaa ccatcttccg ggagtcgtac gcggccatcg tggagcgcgg cgggaagggc
     9781 gaggaggcgt cggcgcgctt cattacccgc atcggcgagc agtatgtgat ggtgacccgc
     9841 gacggggccg aggtgaacgc gcagaccttc ccgagctatg cagtgctcct tgccgccaag
     9901 acgctcaacc cgaagccccg gatgcgctac gtcttttgcg acgacgaggg gccgctggcg
     9961 gcgctcgatg cgctgtaccg gatggggcgc gagcaccaca tcgtgatggc ctacgaggag
    10021 cggggcgagg cgatgctgtg ctacgtgatc gacgagaacg gcaacctgtt ctcgttcatc
    10081 cggcccaccg cgcagcggga cgaggtgctg gtgtacctct acgggttctg ccggagcgtg
    10141 ctcaagcggc tcaaggcgtc gagctcgttt tcactcctgg ccgagggcaa tatcctcgtc
    10201 aaccggctcg ccttcgacaa gttcgggaaa ttcaccgtga gcgacgagtc gatcaacgtg
    10261 cacaagcgct acatcgtggg ctacagcggc gagcgctcga tcgccgtgca ggtcgcgcgg
    10321 cacctctccg acgagacctt ctataacgtg ctcttccccg acggcgtctc gtccggcttc
    10381 atgagcaccg gcgagctcta cgcggtgtca gaccgcatca ggaagctgcg gcaggccggg
    10441 cacgatctcg cgacctgcgt ccgcgacatc accttcaccg acctcaccga ggccgaggaa
    10501 aaactcggca gcaccatcta cctgctggaa aaatacaagc tcgagtccgt tatagagaag
    10561 ggcctgcgac agatgtgacg cctgcgcggc ggcgcttagt actcgccgcc ggggaagttg
    10621 ttgcggaaga agcgcacctt tttcttttct ttgtcggcgg gctggccgcc gagggtgccc
    10681 ttctcgagcg cgatgtccag gagcttcacg ctctcgtcgt cggtgcgcgg cgactcggcg
    10741 gcgattggcg tcggggccgc ttcgcccgcg cgctcgctct gcagcttctc gatcttttca
    10801 tcgatgaggg actgggtgag catggagatt tcctccatgt cctcctcgtc gtcgacgggg
    10861 ggtttttcct ttttgcgcgg ttttttcggc ttcgcctttt tctcgaccgc gggtttcggg
    10921 gcgggacgtt cgggctctcg cgccggcgcg ggttccgcgg cggcatcctc ctcgagatac
    10981 atggccggcg ggttcgcgat cttcagttcc agcagcttga tgcgctcgcc cagcaggatg
    11041 ttgatctgct tctcgatgga catgtagatg ggggtgctga aggacttgtt ggtcttgatg
    11101 gttttctcga gcttctttaa gtcccggatg tcctggtcga tggtttcgat gcgcttgagc
    11161 gcgtgaatga ccttcatggc agacccctgt cgtgcgagcg atatcggtag cgtacactac
    11221 gctatgccgc gcgacgattt ccgtcaatta aaatatgatg gtctggtcct tttcgaccgt
    11281 gaaggaggcg tggcagtcgg ggcagaggta tgatccgctc cgtcggacgc gcaccaggga
    11341 gcggcatttc gcgcattcga cgatgaggga ccgggcgaaa tcgacctcgg cgtcgttcac
    11401 gagcgcgtcg gcgggcggtg cggcatcggt ttcgcgatcc ggcggcccga ccgacagggc
    11461 gtcgtcgcgc gcctgcggcg cattggtggc ggacggctcc gcgatctcga cgacgggcgc
    11521 gctcgtcggc tggtcctcgg ccaacgcggc gtcgatctcc tgcgagggga tcgcgtcggc
    11581 tcccatccct gcgggcgtgg ccgcgggcga ttcgcgcagc tcgttttgct tctcgatgat
    11641 gcgcagcgcc tcgtcgcggg tgtcggtgac ggtgaagacc ttgtcgaacc cgaggatctc
    11701 gtagagggag cggacctcag acggcaggcg atagacgacg agaaagccgt tgcgcgacgc
    11761 gatcgccttt tgcatgtaga gcatgagccc gatgcccgcc gagctcacgt agtcgacgcc
    11821 gcccatgtcc agcgcgatga aggtcttgtt ctgctccagc aggcgggtga ggtagcgttc
    11881 gaaatcgggg ctggtctcgg cgttgacggg gcccttgatc tcgacgatgg tgatcgcgcc
    11941 ccccagcgct tcggcgtggt cgatgaaaaa cattggctac ctctttcgtt tgaccggaat
    12001 ggcgtttttg atcgggcggc gcacgtccac cggggcctgc gtttcgggac gcgggcccgc
    12061 cggcgcaccc tcgggcgcgt gttccccggg ctcgccggcg atctcgcgca cctcgaagcc
    12121 gtcctccggc atgtcgcgca acgggatctc tcgctcgagc gggtcttgct tggcgagggg
    12181 gattgttgcg gccggcgccg gcccgggggc ggcggacacc cgcgcggcca gggcgcggag
    12241 gcgaagataa aaaacgaccg tggtgcagag ggcgatgagg cacgacgccg cggcgagcaa
    12301 cgccgcaagc cgcagcgtct cgccgacggt gcgcgtgaac tcgccgtgga cgcggtcgcg
    12361 cccgatgacg aggaccgtcg cgccgaccgg cgtcgccttg atgacgacgg gccgcaccat
    12421 gagccagccg gaccggtcga tccccggata gaggtgccgc ttcaccagcg gcagcagatg
    12481 gcgcgcatac gggatcgcgg gcagtgcggc cccggagatg tcatactcga tgatcgccgc
    12541 ggcgcgcttg ccgctcgcga tcggttcgag gatccggtcc gcggggtacg agggaccgcc
    12601 gccgggggct gcgccgatag tggtgttcgc aagggcgtcg ttcgtgtgca ggaggatggt
    12661 cccgtcgcgc cgggcgtaga aggcctcgcg gaccgttccg tcggcggcct tctcggcaaa
    12721 gagtgcgcga aggcgcgcga ggtcgccggt cgcgacggac agttccgcaa tgccggcggc
    12781 ggcgtcggcg accaccccgg ccaggcgcag ggtcgcggcc tccgtctcgg cgatgcgcgc
    12841 gcgcgcggtc aggtagccgt atccgccggc gaccgctgcg gagaggccca ggaggagcgc
    12901 cagcatgaac agcagcatgc caatgggaaa ggatggtgtg cggggcgcgc ctgtcattga
    12961 tcgggttccg gtaccggccg tttgggtttc taataatata tatctctatc ggcaggcggc
    13021 ggcgaaattt tcactagtat attgtcgcgg ccgccgcgca gggcgcggat gctcccaaaa
    13081 aaatcattga gcattcacgg catgggggcg atgattgatc gccacaacag tacgcgcggg
    13141 ccggatgcgc ccggtggcgt tatcgacatg cgaaaagagg atggtatgac accgcacacg
    13201 cccgaagaag agatcgagga gtgggactgg acgacggcgc gaccgaccgg cagacccgtg
    13261 ccgcgacggc gtgcgcatcg cgaggggatc gcgcacgagg gcgtgcatct ctgggtgttt
    13321 cgccttgacc gcgaggaccc ggagttgttg ttccagcacc gcgcggcgaa caaggacatg
    13381 tatccgtcgt gcctggatat caccgtgggc gggcatgtgc ccttcgggac ggcggagaac
    13441 aaaatccaga aggaggcctt cgaggagatc ggcatcgcgc ccgaggagcg cgacctcgtc
    13501 gatctcggtt atttccgcta cgaggagttc gagccggggc ggatccatcg cgagttccag
    13561 cgggtgtggc tgtatcggga cgagcgcgcg ctggacggct accgcttcac cgacggcgag
    13621 gtggacgcca tcgccgcggt gccggtgtac gccgtggagg gcatgctgcg caccgacatg
    13681 gggtttcttg cggaatggtg cgcgggcggc cggatcgaga cgcgcgaact gtcgcgcaaa
    13741 gactttcacc cgcttctctt cgcgccgtcc atggcggcgt acatggacgt ggtgatcggc
    13801 gcggtgaagg agtacgcccg ccggggcgcg gtgtcggtgc ggatgcccgc gccgtagcgt
    13861 gtaacggttc gcggcgcgtg tcgaatccct tgacagatgt gcgatcaggg tgtatcttgt
    13921 ggtgtgggcg cgatggcgat ttcacgcgcg ccatacaccc gcccggagag gtgccgtcat
    13981 taacgaaacg atcctttacc tcgatgttgt cgatgcgcat ggcgggctca agcccgaatt
    14041 cctcgcgtcg ttcacgagcg gcgagcgcga ggtcctcgac cgaatcaatc gtacggtggc
    14101 cgacgggaac tcgctgcagg aggtgatcgg cgcctatttc gacgaggtgc gcaccctcct
    14161 accgtgcaat cgcctcgatg tcgcctttct ggaagaagga gacgagcgct tggtgctgca
    14221 gtactcgatt gccgattacg agccgctgca tctgcagcgc ggatacgcct ccgacatcgc
    14281 cggcagcccg gtcgagaaaa tcgtgcacga gcgttgtgcg agcatcatcc acgatatggc
    14341 ccgctatgcg gaggaaaatc cggtgagcgc ctcggcgcgt cttctcctgc aggaggggat
    14401 ccgatccagc atgacctgtc cgctgtccgt ggagaaccgg ccggtggggc ttatcctctg
    14461 ccgctcgagt cgcgtgaacg ccttcgacga gcgctcggtg ctgctgcatt tcgcggtcgc
    14521 ggaacggctg tcgcaggccg tcgagaaggc gtatcggctc gaccagctcg attcggtgct
    14581 gcagaactac atggaaatgc tctccttcgt gagccatgag atcaagagtc cgctcgcgtc
    14641 gatcatcacc ctggggaaaa ccctcgcctc cggttacttc ggcgcgatcg acgagcggca
    14701 ccgcgatatc gtcacccgca tcgtccagaa ggcggagtac ctgcacaatc tcagcgtgga
    14761 gtacctcaat ctcgcccggt tcgaaagcgg gcaaatggag ctcaaggcgt atcccgtcca
    14821 gttcttcgac gaggtgatct ctcccgccat cgagatcatc gccccccagc tcttcgagag
    14881 ccatgttgaa tttttccgcg acctgccgca cgagatggag ccggttacgt gcaacccgga
    14941 gctcatgaag atcgtcctgg tgaacttcct ctccaacggg atcaagtacg gcaggccgcg
    15001 cggcacgctc aagctcacgg tgcgttacct gaaccggcgg gtgcggtttt ccgtgtggaa
    15061 cgagggcgcg ggcttcccgc cggaggatcg agtgaagctt tttaagaaat tctccacgct
    15121 gaacattccg ggccgcgagc gtatcaaggg ccacggggtg ggtctgtatg tgtgctggaa
    15181 aatagtccat ctccacggcg ggcacatctg ggccgattca gagtacggcg cctgggccga
    15241 gttcacggtc gagatcccgc agttcggcga catgtgcatc atcggcaagg ccgattgacg
    15301 gcgcgcgccg ccatgcgatg cgtgcgtcag tggaaagcgc tcatggttgc ggacgcggtt
    15361 tttttgcttc cgcgggcccg atgcgctacg ctgtcacggg agcgaaatcg gatatgcgct
    15421 tcgcgtgatc gaaatttcac aaacatggga gcggtgtgat gaaaaagaaa ctttactcga
    15481 tcctctgcgc cgcgggtctc gtctcgtcgg cggcatgcca gtcggcgccc ggcatggaaa
    15541 cggtgcccta tgtggacctg ccgcgattca tgggcgattg gtacgtgatc gcctgcatt
//
LOCUS       DAORHF010000065        15530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2890_length_15530_cov_4.767754, whole genome shotgun sequence.
ACCESSION   DAORHF010000065 DAORHF010000000
VERSION     DAORHF010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15530
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2890_length_15530_cov_4.767754"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="PKJ16_07450"
     CDS             complement(<1..549)
                     /locus_tag="PKJ16_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HNV46862.1"
                     /translation="MKTRGNGRDIILQAFHWNLVKTQGTGTIDGRAESWYRILFDMAD
                     DLAEIGFTIVYLPPPWRDDSRWESEGRHGGGEGYFWHDFDLNSRYGTKRELIDLVDAL
                     HNRGIRVIVDLVTNHRDGARMTDDQWEYPGECWNIGGFDDGGAFMDGRYDLNLGEVRV
                     YTRIKKAMNELLNECGVDGWRWD"
     gene            861..1598
                     /locus_tag="PKJ16_07455"
     CDS             861..1598
                     /locus_tag="PKJ16_07455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV46863.1"
                     /translation="MDTAHTQAPSIRNRSDKRFIKRFKERVRRQALAHPGALFLEGSA
                     DGARIALTFDDGPDAKYTPIVLSILRKHGVKATFFMRGRRVRRHPDLVRAVDRAGHAI
                     GIHSLTHTAIDALPAPEAYEREVRFMGDVFAALVGHRPAIYRPPYGLVSDEQIAYFAA
                     RGVRTVIWSVDSNDWDTRTLSAQGIARTVLRYAHPGAIVLMHDACNERCFTHRALETI
                     IITLKKRGYSFCTVPELIGGAHRPAQN"
     gene            1657..2580
                     /locus_tag="PKJ16_07460"
     CDS             1657..2580
                     /locus_tag="PKJ16_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46864.1"
                     /translation="MKITSRLERLAADALSESLDVRNMDLLVRSIDQYYDLYASTGFP
                     ANVPIPKITAAKQIVSDIKNGNKFLQLVEQLVEYHYVGHMGRTYQIAYLREIVKELRD
                     QGLIYDTENKIFVEDPAVRRTRNWGTLRDGDEHLFTFLRIDIVANTELVKRHSADAVR
                     ATYDDLRAIVVQAVERRNGRIWSWEGDGGLAAFFFGKKHLMATLGAMEIAHELFFYNL
                     TRCRLDGALEVRVAVHSGQCQYAESIDDLQKSDTIRKLVEIESQHTLPNTVSISHATH
                     VMLDALIASRFAQIPGESRAMHYRYELCWEQ"
     gene            2580..3557
                     /locus_tag="PKJ16_07465"
     CDS             2580..3557
                     /locus_tag="PKJ16_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46865.1"
                     /translation="MDIIVFSDNKNINGCFAALKKVSSITLAVHPVKSLKKAIAAAAR
                     AIVYLDVSGLTAAERSRSIKTLSAMRDNRFAIIDPKGAVDDVGELFFAGAADYVGKKL
                     YAQGITLKRIQRLMAFRPIDLPGEAPEAAAPCLFDAPLSGADWSTVRDDCEYTFCFMF
                     IELDHLMEQKERIGAHHVDKLSAQFMAHIEKKMEPLHGRLWMPQEYGGVILFPFDGAR
                     CDAILACFRMVMDRHIMMTEDLGLDTLLSFRIVLHVGSTVYRGRGSTGTIVSDTINSI
                     FHLGKKFAEPGNFYLTPTAARYIPEWLIDYFLHAGEFEGREITRMRLNR"
     gene            complement(3558..5066)
                     /locus_tag="PKJ16_07470"
     CDS             complement(3558..5066)
                     /locus_tag="PKJ16_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV46866.1"
                     /translation="MIHDRSKRAALRTSPRWGIATLLCAAIGISCATGPRYSEFSPTI
                     SADDRMMIFQSDREKRHRFRLYYKQKIDGQWREPVFMEGLGAVEGDGGPFITYDQNYL
                     VFSSLRKGGAGNLDLWISQRLGTTWGPPVNMGAPINSSGYDGFGTISPDGKTIFFVRQ
                     CPEKKHGGEDSFGIFFSVKDDAGKWTEPKKMSAPVNGEYSDFGPVILADNLSLIFSSN
                     RPGGLGKYDLYKTERLNDGQWTEPVNLGPGINTPGDDMLVSIPASGSVVYFGRTDNEK
                     DDIYRIHSMPIPEKLQHTTVLIVRGTVRDKRDPSLTLAAGITITDTRSDEKPITIRSN
                     EKDGAYIVILKKGTLYDVAAACPGYTFHSQRIDLTKLEKFKETEENILLEPLTVGTNL
                     VLNNLFFKYKSYALLPASKYELQRVKKLMDENPTLRIEIGGHTDTIGPKRYNMKLSQQ
                     RAQSVVDYLAEQGVAKERLDAKGYGPDKPIGDNETEEGKEKNRRVEIMVLEK"
     gene            5290..5718
                     /gene="flgB"
                     /locus_tag="PKJ16_07475"
     CDS             5290..5718
                     /gene="flgB"
                     /locus_tag="PKJ16_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HNV46867.1"
                     /translation="MFESSQMMRNNYLLERGLDTESLRRKVISNNIANVDVPHFKRSE
                     VNFESELKRVINDNARQDHKYPALMSDDRHIPFYEERDIRSVRPRVNLDYNTTYRNDG
                     NNVDIEKEMVDASKNAMRYNAFLTGLNQNYKVLKLIMRQV"
     gene            5731..6195
                     /gene="flgC"
                     /locus_tag="PKJ16_07480"
     CDS             5731..6195
                     /gene="flgC"
                     /locus_tag="PKJ16_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014653522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HNV46868.1"
                     /translation="MGMFDSFNIASTGLTAQRLRMDLISNNVANANTTRTTEGGPFVR
                     KLAIFAPVNHRPFYKSPLVPERIDHGSGRGVRVVKIEKDKAPFRLVYDPTHPDAIAIG
                     PKKGYVEMPNVNIVSEMTDLISASRSYEANITMVNNSKMMFNRALEIGRGGV"
     gene            6210..6353
                     /locus_tag="PKJ16_07485"
     CDS             6210..6353
                     /locus_tag="PKJ16_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46869.1"
                     /translation="MNNEKPGKMEVNISHNMSNRDNYRKVVAVKSVTAELIRNSFVDM
                     RYF"
     gene            6376..6705
                     /gene="fliE"
                     /locus_tag="PKJ16_07490"
     CDS             6376..6705
                     /gene="fliE"
                     /locus_tag="PKJ16_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HNV46870.1"
                     /translation="MKITNDNPSVGTMVNLRTTDPLHFSSKQQRPIDDDVAGSFGEML
                     TAALSKVNNIQVESDKLTQQMIHAPESVDIHSVMIAGQKAEIAISFTKAVRDEVIRGY
                     RELMNMR"
     gene            6912..8612
                     /gene="fliF"
                     /locus_tag="PKJ16_07495"
     CDS             6912..8612
                     /gene="fliF"
                     /locus_tag="PKJ16_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HNV46871.1"
                     /translation="MVEFFKKLLDQAKELFAKLDTTKKIIIGSVFGVVVVAFIVLFSI
                     SSGRPEVLLFSDLPADEFGRVTKKLEEMGHSYSTKGTTSVFVRPEKREVILTQLAQEN
                     MIPKGIPGWKLFDMSKWTETERELDVKYMRALRDEVKRHIESLKNIEKADVEIAITKD
                     ELFTDKDNPYTAAVTVYLAPGYDKLSKKEIKGITYLVSRAVGGKLKPENVTVTDEMGK
                     IISDFDDELDAAKTEFTKIEYRQKIEEKIKAQMIRNIKKAFEAIYTSDRIQVVNLDMQ
                     FNWDKVSEEREEHFPIEMEKDNPATPYSERKVKDSLVISEKATKEHFQGHGWNPEGPA
                     GTEGNKPPGYKASDDQYAKYDKQENITNHAINKSKKKIEKDPYDLDRISVAIAIDSVQ
                     DLPRKPDGSYDLDPGKKPVQTPLTKDELKQAENILKKAVNFNDIRGDQVAVENITFDR
                     TKQWNALRDEYMRKEQMKRMLLAALVGVFALFIGVLLFRAINKELARRRRLREEQLAA
                     EQQRMREAALRAAEEEGVDVELSLEDKARLELQENAINLARERPDDVAQLLRTWLSED
                     "
     gene            8785..9801
                     /gene="fliG"
                     /locus_tag="PKJ16_07500"
     CDS             8785..9801
                     /gene="fliG"
                     /locus_tag="PKJ16_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNV46872.1"
                     /translation="MLQSKKTQLTGRQKAAVFLVSLGSDVSSEIFKHLREDEIEQLTF
                     EIARLDKVDPEDKDRVLQEFQELMMAQEFISTGGIDYAREVLERALGTQKAIDIVNRL
                     TSSLQVRPFDFIRRTDPSHLLNFIQGEHPQTIALILAYLDPSKASTILGGLSHQIQAD
                     VAKRIATMDRTSPDVLREVERVLERKLSTLASEDYTSAGGVDAIVEILNQVDRGTEKI
                     IIEALEEEDPELAEEIKKRMFVFEDIVLLDDRSIQKVLREVDTQDLAKALKGVDAEVQ
                     EKIFRNMSKRASSLLREDMDFMGPIRLRDVEEAQQKIVNIIRKLEEAGDIIVARAGEE
                     ELVV"
     gene            9794..10720
                     /gene="fliH"
                     /locus_tag="PKJ16_07505"
     CDS             9794..10720
                     /gene="fliH"
                     /locus_tag="PKJ16_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HNV46873.1"
                     /translation="MSRLVFKPGEIVVTGAPKQVELPDKYKKNVMTDEEYKEFEVDES
                     GNPVDVYQGPSIEEMEAELERYRRETEEEVSRMLDDAREKAKRIEEDGRQAAFKEMQN
                     AREEIKGELEKFRIDSEREIERGKFEAEKIIKEAELKVSEIEHEAYKKGYDAGREEGY
                     KEGQAEVMRLIDRLGTIVSTAVDIRDDIIRSSEKLMTEMILMIARKVIKDEIVERREV
                     VINNIKEAIKRVKDRDRIDIRVNFADLDMTTAHKDELIKLMESLKKVNIYEDSRVERG
                     GCIIETDVGAIDARISTQLDTIEEAIRNTSTV"
     gene            10901..12307
                     /locus_tag="PKJ16_07510"
     CDS             10901..12307
                     /locus_tag="PKJ16_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HNV46874.1"
                     /translation="MIRILPHDKVDILSKYKKIIDETELLKFTGYVERVVGLTVESVG
                     PYVQYGEICKIKLRDKRYLPAEVVGFHKNRVVLMPIGEMSGVMPGAEVIAMGSALMVP
                     VGEELLGRVVNGVGEPLDGKGRIFTKAKYPVDGAAPNPMKRSIIDRPLSVGIRAIDGM
                     LTVGRGQRIGIFSGSGVGKSTLLAMIARYTDADYNVIALIGERGREVRDFIEKELGTE
                     GLARSVVIVATSDQPPMVRLRGAFLAHAVAEYFRDLGKDVNILMDSVTRFAHAQREVG
                     LASGEPASTRGYTPSVYKILPRLLERSGTREGGGSITGFYTILVEADDMNEPIADAVR
                     GILDGHVVLDRNLAHKGHYPAIDVLGSISRCMKDVVDDDHKAAAARFRELLAAFRDKE
                     DLILLNAYARGADPVVDKAIEMMGDLNAFLRQGIHERDEYETIRARLIGLFRGEEKPA
                     ARADAVTPAYYQRFRAGA"
     gene            12409..12888
                     /locus_tag="PKJ16_07515"
     CDS             12409..12888
                     /locus_tag="PKJ16_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46875.1"
                     /translation="MFVTVEDVRELGVAVKGSSEKAKVDKFVFARSLTWLGGFVLYEY
                     EYDGKEKSSLYIYNSINKLNAMSSFTGKYFKKAGFAAAFSKKGIVLKELGELKQRYPN
                     FSVSIILNEGKPIGNLVSANDGTIDYTLILTGVFLDEPEIWAELIDKKTAAMNAVMR"
     gene            12902..13375
                     /locus_tag="PKJ16_07520"
     CDS             12902..13375
                     /locus_tag="PKJ16_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46876.1"
                     /translation="MKKFVFKLETLLAIKAAREKEIQNELAALVSIQNAERMRQDEYR
                     AKIAREQESFNAKLRSGRFAYADAMMYERFVEFAGKVIVAAQGKIEAMEPEIQKVRDR
                     LVQASTERRVIEKLKERQWKAHRYQVEVELAKENDDANQKVHLRRKIEMEREIRS"
     gene            13372..13953
                     /locus_tag="PKJ16_07525"
     CDS             13372..13953
                     /locus_tag="PKJ16_07525"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV46877.1"
                     /translation="MMEDMFSVMGRIVEIKKRFGLMNHPYTRQRGADFSEQVRRRLDE
                     ADAPRRDEQATVDVAAGKDMTVPEMKKLAQVYAGQKRVPPSLVDAVIQVESNYNPQAV
                     SPKGALGLMQLMPDTVGSLGISDPFDPAENIRGGVSILQRLLQKYGGDYTKALAAYNA
                     GERRVDESNGVPNIRETKDYVEKVINAYLKNMK"
     gene            13968..14561
                     /locus_tag="PKJ16_07530"
     CDS             13968..14561
                     /locus_tag="PKJ16_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46878.1"
                     /translation="MRAISDRAKLLYILLLIMFIVVIGFFWLDHIGFINLKKMFGVKK
                     DAVSVLDAKGDEPSLVEREEFEKEKLKVGERVEDLDKREAAIQEAEKTLHTDREKLEE
                     IRKGLELEKNKIEKEKKQYSGYKRNVVDLAQKIENIPPEQAVEILIRWEDTLVIDVLR
                     QIDFNATEAGKMSISSYLISLMPKDKASRIMYLMTQL"
     gene            14849..15361
                     /locus_tag="PKJ16_07535"
     CDS             14849..15361
                     /locus_tag="PKJ16_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46879.1"
                     /translation="MKCILNNEFVPYINNGTLNVFQIRCLVEIKEFVDRIAGRQYLDG
                     RTVASLTEKYGATPTVTTWGDYFQTEIGFQLLGKSDEEFRQAVDTVKFDVISSHIIFT
                     GKGIDFFEWVENNYQDYINRKDELSFREYEEIVHLKILKDYFVNLNITDAFTYDEMKW
                     YGGYREAAAI"
BASE COUNT         3340 a         4489 c         4823 g         2878 t
ORIGIN      
        1 gtcccaccgc catccgtcga cgccgcactc gttcaggagc tcgttcatgg ccttcttgat
       61 tcgtgtgtac acccgcactt cgccgaggtt gagatcgtac cgtccgtcca tgaaggcgcc
      121 cccgtcgtcg aagccgccga tgttccagca ttcgccggga tactcccatt ggtcgtccgt
      181 catccgggcg ccatcgcggt gattggtcac caggtccacg ataacccgaa tgccgcgatt
      241 gtggagcgcg tcgacgaggt cgatcagctc acgcttggtg ccgtaccggg agttgaggtc
      301 gaagtcgtgc cagaagtagc cctcgccgcc gccgtgcctt ccctcggact cccagcggga
      361 atcgtcgcgc caggggggcg ggagatagac gatggtgaag ccgatctccg caaggtcgtc
      421 ggccatgtcg aaaaggatgc ggtaccagga ttcggcgcga ccgtcgatgg tcccggtccc
      481 ctgtgttttc accaggttcc agtgaaacgc ctggaggatg atgtcgcgcc cgttgccccg
      541 tgttttcatc ggtggtgcgc tccttgtcag atatcgagtc acatacgtca gtgatgatgg
      601 cggatgtcgc accgtcccgc aagcgttttt cgatccgaaa cccgcattgg cgtcgaaaaa
      661 cttattgccg gcacgatcgc gtatcggaat aacagcgtat gcgtgaacat ggaatgatac
      721 gacagcggct tcgggaccgg ctgaagggag cgcgtgcagc agtgtcttcg acgaaacgaa
      781 cggtgcgata cgctgtcgca cttgccgcgc tgatcgcgtg catgggctac gcggtttcgg
      841 atggggggga gcgtcgagcg gtggatacgg ctcacacgca ggccccctcc atcaggaacc
      901 gctccgataa gcgcttcatc aaacggttca aggagcgggt gcggcggcag gcgctcgcgc
      961 atccgggggc gctctttctc gaggggtccg ccgatggcgc gcggatcgcg ctcaccttcg
     1021 acgacgggcc ggacgcgaaa tacacgccca tcgtcctgtc gatcctgcga aagcatggcg
     1081 tgaaggcgac cttcttcatg cggggacggc gggtgcgccg gcaccccgat ctcgttcgcg
     1141 ccgtggaccg cgcgggacac gcgatcggca tccacagcct cacgcacacc gcgattgacg
     1201 ccctccccgc gccggaggcc tacgagcgcg aggtgcgctt catgggcgac gtgttcgcgg
     1261 cgctcgtcgg ccatcgaccg gcgatctatc gtccgccata cgggctggtg tccgacgagc
     1321 agatcgccta cttcgccgcg cgcggcgtcc ggaccgtcat ctggtcggtg gactcgaacg
     1381 actgggacac gcgaacgctc tccgcgcagg gcatcgcgcg cacggtgctg cgctacgccc
     1441 atcccggggc gatcgtcctg atgcacgatg cctgcaacga gcgttgtttc acccaccgcg
     1501 cgctcgagac gatcatcatc accctgaaaa agagaggcta ctcgttttgc acggtccccg
     1561 aactcatcgg gggcgcgcac cggccggctc aaaactagtt gacaataatc cggcgttgcg
     1621 gcacgcttcg gacgactacg tggtgcggat ggacacatga aaataacctc gcggctggag
     1681 cgcctggcgg ccgacgccct ttccgaatcg ctcgatgtgc ggaacatgga cctgctggtg
     1741 cgctccatcg accagtacta cgacctctat gccagcaccg gatttccggc caacgtcccc
     1801 atcccgaaga taacggcggc caagcagatt gtgagcgata tcaagaacgg caacaagttc
     1861 ctgcagcttg tcgagcagct ggtggagtac cactatgtcg gccacatggg gcgcacctat
     1921 cagatcgcct atctgcgcga gatcgtcaag gagctgcgcg accagggcct catctacgac
     1981 accgaaaata aaatcttcgt cgaggacccg gcggtgcgcc ggacgcgcaa ctgggggacc
     2041 ctgcgcgacg gcgacgagca cctcttcacc ttcctgcgca tcgacatcgt ggccaacacc
     2101 gagctcgtga agcgccattc cgccgatgcc gtccgcgcga cctacgacga cctgcgcgcc
     2161 atcgtcgtcc aggcggtgga gcggcgcaac gggcggatct ggagctggga gggcgacggg
     2221 gggcttgcgg ccttcttctt cggcaagaag catctcatgg cgacgctggg cgcgatggag
     2281 atcgcgcatg agcttttttt ctacaatctc acccgctgcc ggctcgatgg cgcactggag
     2341 gtccgcgtcg cggtgcatag cggccagtgc cagtacgcgg agagcatcga cgacctgcag
     2401 aagagcgaca ccatccggaa gcttgtcgag atcgaatcgc agcacaccct gcccaatacg
     2461 gtgagcataa gccacgccac gcacgtgatg ctggacgccc tcatcgcgtc gcgcttcgcg
     2521 cagatccccg gggagagtcg cgccatgcac taccgctacg aactgtgctg ggagcagtga
     2581 tggatatcat cgtcttttcg gacaataaaa acatcaatgg ctgtttcgcg gccctcaaaa
     2641 aggtttcgag cataaccctg gccgtgcacc ccgtgaagag tctcaagaag gccatcgccg
     2701 cagcggcgcg cgccatcgtc tacctggatg tttccggact caccgccgcc gagcggtcgc
     2761 ggtccattaa aacgctttcc gcaatgcggg acaaccggtt cgccatcatc gatcccaagg
     2821 gcgcggtcga tgacgtcggc gagctcttct tcgcgggcgc cgcggactac gtggggaaaa
     2881 agctctacgc gcaggggatc accctcaagc gcatccagcg cctcatggcg tttcggccca
     2941 tcgacctgcc gggcgaggcg cccgaggcgg ccgctccctg cctgttcgac gcgccgctct
     3001 cgggggcgga ttggtcgacg gtgcgcgacg attgcgaata caccttctgc ttcatgttca
     3061 ttgagctgga ccatctcatg gagcagaagg agcggatcgg cgcccatcac gtggacaagc
     3121 tttcggcgca gttcatggcg catatcgaaa aaaagatgga gccgcttcac ggtcggttgt
     3181 ggatgccgca ggagtatggc ggggtgatat tgtttccctt cgacggggcg cggtgcgatg
     3241 ccattctcgc ctgctttcgc atggtcatgg accggcatat catgatgacc gaggacctgg
     3301 ggctcgatac gctgctgtcg ttccgcatcg tgctgcatgt cggaagcacc gtataccggg
     3361 ggcgggggag caccggcacc atcgtgtccg acaccatcaa ctcgatcttt cacctgggca
     3421 agaagttcgc cgagccgggg aacttctatc tgaccccaac ggcggcgcgg tatatccccg
     3481 aatggctcat cgactacttc ctgcacgcgg gcgagttcga ggggcgcgaa atcacccgca
     3541 tgcgcctcaa tcgctagcta tttttccagc accatgatct cgacccgacg gttcttctcc
     3601 ttcccctcct cggtttcatt gtcgccgatg ggcttgtcgg gcccgtaccc cttcgcgtcg
     3661 agccgctcct tcgcgacgcc ctgctcggcc agatagtcga ccaccgactg cgcgcgctgc
     3721 tgcgacagct tcatgttgta ccgcttgggg ccgatggtgt cggtgtgccc gccgatctcg
     3781 atgcgaagcg tggggttctc gtccatgagt tttttcacgc gctggagctc gtatttcgac
     3841 gccgggagga gggcgtagct cttgtacttg aagaagaggt tgttgagcac gaggttggtc
     3901 ccgacggtga gcggctccag gaggatgttt tcctcggttt ccttgaactt ctcgagtttc
     3961 gtcaggtcga tccgctgcga gtggaaggtg taccccgggc aggcggcggc cacgtcgtag
     4021 agcgttccct ttttcaggat cacgatatag gcgccgtcct tctcgttgct ccggatggtg
     4081 atgggcttct cgtcgctccg ggtgtcggtg atggtgatcc ccgccgcgag cgtgagcgac
     4141 ggatcgcgct tgtcgcgcac cgtcccgcgc acgatgagca ccgtcgtgtg ctgcagcttc
     4201 tccgggatgg gcatcgagtg gatccggtag atgtcgtcct tctcgttgtc ggtccgccca
     4261 aaatagacga cgctccccga agccgggatg ctcaccagca tgtcgtcgcc gggggtgttg
     4321 atcccggggc ccaggttcac gggctcggtc cattgcccgt cgttgagccg ctcggttttg
     4381 tagagatcgt atttccccaa gccccccggg cggttcgagc tgaagatgag cgacaggttg
     4441 tcggcgagga tcaccgggcc gaaatcgctg tactcgccgt tgacgggcgc ggacatcttt
     4501 ttcggctcgg tccattttcc cgcatcgtcc ttcaccgaga agaatattcc gaacgagtcc
     4561 tcgccgccgt gcttcttttc cgggcactgg cgcacgaaga agatggtttt accgtcaggt
     4621 gagatggtgc cgaagccgtc atatcccgag gaattgatcg gcgcgcccat gttgaccggc
     4681 ggtccccagg tcgtcccgag gcgctgcgag atccacaggt ccaggtttcc cgcgcccccc
     4741 ttccgaagcg acgagaagac caggtagttc tggtcatagg tgataaaggg accgccgtcg
     4801 ccctcgaccg cgccaagccc ctccatgaac acgggctccc tccactggcc gtcgatcttc
     4861 tgcttgtaat agagacggaa gcgatgtcgc ttctcccggt cgctttggaa gatcatcatc
     4921 cgatcgtccg cggagatggt ggggctgaac tccgaatagc gggggcccgt ggcgcacgag
     4981 atccctatgg cggcgcacag cagcgttgcg atcccccacc ggggcgacgt gcggagcgcc
     5041 gcgcgcttcg atctgtcgtg tatcatattt tcctccccag gccggcggca atgcgtttct
     5101 cccaatgttc gagcgccgac catcgccact gtgaaagggg attgacgcct gtgtccatga
     5161 aaaaacgaac cctcgacgcg aggaatttcc cattatgtcc cataaaaaaa ttctcatgct
     5221 tttttttaaa aagccgaaat gagaaatgag ggcgaggaat ctatcgcact atacatccgt
     5281 gaggcggtca tgttcgaaag cagtcagatg atgagaaaca actatctgtt ggagcgcggg
     5341 ctcgataccg aatcgctccg acggaaggtg atatccaaca atatcgcgaa cgtcgatgtc
     5401 ccgcacttca agcggagcga ggtgaacttc gaaagcgagc tcaagcgcgt catcaacgac
     5461 aatgcgcgcc aggaccacaa gtatcccgcg ctcatgagcg acgatcggca catcccgttc
     5521 tacgaggagc gcgacatccg cagcgtccgg ccccgcgtga acctcgacta caacacgacc
     5581 tatcgcaacg acgggaacaa cgtggacatc gagaaggaaa tggtggacgc gtccaagaac
     5641 gccatgcgct acaacgcgtt tctcaccggt ctcaatcaga attacaaggt cctcaagctc
     5701 atcatgcggc aggtgtaggg gaggtagatg atgggaatgt tcgatagctt caatatcgcc
     5761 tcgaccggcc tcacggccca gcggctcagg atggacctca tatcaaacaa cgtggcgaac
     5821 gccaacacca cgcggaccac cgagggcggc cccttcgtcc gcaagctcgc catcttcgcg
     5881 ccggtgaacc accggccgtt ctacaagtcg ccgctggtcc cggaacgcat cgatcacggt
     5941 tcgggcaggg gcgtgcgcgt ggtcaagatc gagaaggaca aggcgccctt ccgcctggtg
     6001 tacgatccca cgcacccgga cgccatcgcg atcgggccca agaagggata cgtggagatg
     6061 ccgaacgtga acatcgtttc agagatgacc gatcttatct cggcgtcccg ttcgtatgag
     6121 gcgaacatta ccatggtaaa caattcgaag atgatgttca atcgagccct tgagatcgga
     6181 cggggcggcg tataatccgg aggtgaggca tgaataacga gaaaccgggc aagatggaag
     6241 tgaatatttc gcacaacatg tcgaatcggg ataattatcg caaggtggtc gcggtcaaga
     6301 gcgtgaccgc cgaactcatc cgcaattcgt ttgtcgatat gcggtatttt taaacgacag
     6361 aaacaggagt gtaaaatgaa aattacaaac gacaatccct cggttggcac catggtcaat
     6421 ctgcggacga cggatccgct gcatttttca tcgaagcagc agcggccgat cgatgacgat
     6481 gtcgccgggt ccttcggcga gatgctcacc gccgcgcttt ccaaggtgaa caatatccag
     6541 gtggaatccg acaagctcac gcagcagatg attcacgccc cggagtcggt ggacatccac
     6601 tcggtcatga tcgcggggca gaaggcggag atcgccatca gctttacgaa ggccgtgcgt
     6661 gatgaggtta tacgcggata tcgcgaactc atgaacatgc gataggccac gccgcgccgc
     6721 aattgaacgc ccctcccgat tcgggagggg cggaaaataa tctacaaatt taaaatattt
     6781 gataaatttt tttgtttaca attttactta tatatgtaaa ctggattatg tctctttaac
     6841 gcagtgcgga caatatttac ccattcgggc aatcctggat cgggcccgtg cggccgtggg
     6901 aggagggggc tatggttgag tttttcaaaa agctattgga tcaggcgaag gagctgttcg
     6961 ccaagctgga caccaccaaa aagatcatca ttggctcggt gttcggggtg gtggtggtcg
     7021 ccttcattgt gcttttttca atttcgagcg gccgtcccga ggtgctgctc ttctcggacc
     7081 ttccggccga cgagttcggg cgggtgacca agaagctcga ggaaatgggc cacagctact
     7141 ccaccaaggg caccacgtcg gtcttcgtgc ggccggaaaa gcgcgaggtc atcctcaccc
     7201 agctcgccca ggagaatatg atccccaagg gcattcccgg ctggaagctc ttcgacatgt
     7261 cgaaatggac ggagaccgag cgcgagctcg acgtgaaata catgcgggcc ctgcgcgacg
     7321 aggtgaagcg gcacatcgag tcgctcaaga acatcgaaaa ggccgatgtc gagatcgcca
     7381 tcaccaagga cgagctgttt accgacaagg acaatcccta taccgcggcg gtgaccgtgt
     7441 atctcgcccc cggctatgac aagctcagca aaaaggaaat caagggcatc acctacctcg
     7501 tgtcgcgggc ggtcggcgga aaactcaagc cggaaaacgt gaccgtgacc gacgagatgg
     7561 gcaagatcat ttccgatttc gacgacgagc tggacgccgc caagaccgaa ttcaccaaga
     7621 tcgagtaccg gcaaaagatc gaggaaaaga tcaaggccca gatgatacgc aacatcaaga
     7681 aggccttcga ggcgatatac accagcgacc gcatccaggt ggtgaacctg gacatgcagt
     7741 tcaactggga caaggtgagc gaggagcgcg aggagcattt ccccatcgag atggagaagg
     7801 acaatccggc gaccccctat tcggaacgca aggtgaagga ctcgctggtg atctcggaaa
     7861 aggccaccaa ggagcatttc caggggcatg gctggaaccc ggagggcccg gcggggaccg
     7921 aggggaacaa gccgcccgga tacaaggcga gcgacgacca gtacgcgaag tacgacaagc
     7981 aggagaacat caccaaccac gcgatcaaca agagcaagaa gaagatcgag aaggacccgt
     8041 atgatctcga ccgaatttcg gtcgccatcg ccatcgacag cgtgcaggac cttccgcgca
     8101 agccggacgg cagctatgac ctcgatcccg gcaaaaaacc ggtccagacg ccgctcacga
     8161 aggatgagct gaagcaggcc gagaacatcc tcaagaaggc ggtcaacttc aacgacatcc
     8221 ggggcgacca ggtcgcggtc gagaacatca ccttcgaccg gaccaagcag tggaatgcgc
     8281 tgcgcgatga atacatgcgc aaggagcaga tgaagcgaat gctgctcgcg gcgctcgtcg
     8341 gggtgttcgc gctcttcatc ggcgtgctcc tgttccgggc cataaacaag gagcttgcgc
     8401 ggcgccgccg cctgcgcgag gagcagctcg cggccgaaca gcagcgcatg cgcgaggcgg
     8461 cccttcgcgc ggccgaggag gagggggtcg atgtcgagct atcgctcgag gacaaggcgc
     8521 gcctggagct ccaggagaac gccatcaacc tggcgcggga gcgcccggac gatgtggccc
     8581 agctcctgcg gacgtggctt tctgaggatt aaaggctcaa tacggccggt tttttggccg
     8641 atcatgtgag tagcgcggcg aagtatattt tatttgacac cgcgtcgttc gcgacggtat
     8701 actagcatcg cgccgcgcgc gcggtcggag agacaatgaa ggtggagccg gtgcggcttc
     8761 accttcggcg ggtgtaacgg gaccatgctc cagtcgaaaa aaacgcaact gacgggacgg
     8821 caaaaagcgg cggtctttct ggtgtcgctc gggtccgacg tgtcctcgga gattttcaag
     8881 cacctgcgcg aggacgagat cgagcagctc accttcgaga tcgcccgtct ggacaaggtc
     8941 gatcccgagg acaaggaccg ggtgctccag gagttccagg agctcatgat ggcccaggag
     9001 ttcatctcca cgggcggcat cgattatgcg cgcgaggtgc tggaacgggc gctcgggacg
     9061 cagaaggcga tcgacatcgt gaaccggctc acctcgagcc tgcaggtccg tcccttcgac
     9121 ttcatccgtc gcaccgaccc gagccatctt ctgaacttca tccagggcga gcatccgcag
     9181 accatcgccc tgattttggc ctacctcgat cccagcaagg cgtcgaccat cctcgggggg
     9241 ctgtcgcacc agatccaggc ggacgtcgcc aagcgcatcg ccacgatgga ccgcacctcg
     9301 ccggacgtgc tccgcgaggt ggagcgcgtg ctcgaacgca agctctcgac gctggccagc
     9361 gaggactaca cctcggcggg cggcgtggac gcgatcgtcg aaatcctgaa ccaggtggac
     9421 cgcggaacgg agaagatcat catcgaggcg ctcgaggagg aggacccgga gctcgccgag
     9481 gaaatcaaga agcggatgtt cgtcttcgag gacatcgtcc tgctcgacga ccgttcgatc
     9541 cagaaggtgc tccgcgaggt cgatacgcag gacctcgcca aggcgctcaa gggcgtggac
     9601 gccgaggtgc aggaaaagat cttccgcaac atgtccaagc gcgcctcctc gctcctgcgt
     9661 gaggacatgg acttcatggg tcccatccgg ctgcgcgacg tggaggaggc gcagcaaaag
     9721 atcgtgaaca tcatccgaaa actcgaggaa gccggcgata tcatcgtggc gcgcgcgggc
     9781 gaggaggagc tggttgtcta gattggtttt caagcccggc gagatcgtgg tcacgggggc
     9841 ccccaagcaa gtcgagctcc ccgacaagta taaaaagaac gtcatgaccg acgaggagta
     9901 caaggaattc gaggtcgacg agtccggcaa cccggtcgac gtgtaccagg ggccgtccat
     9961 cgaggagatg gaggccgagc tcgagcgcta ccgacgcgag acggaggaag aggtctcgcg
    10021 catgctcgac gatgcccgcg agaaggcgaa gcggatcgag gaggacggcc ggcaggccgc
    10081 cttcaaggag atgcagaacg cccgtgagga gatcaagggc gaactcgaga agttccggat
    10141 cgattccgag cgcgagatcg agcgcggcaa gttcgaggcg gagaagatca tcaaggaggc
    10201 cgagctcaag gtatccgaga tcgagcacga ggcgtacaag aagggctacg acgccgggcg
    10261 cgaggaaggc tacaaagaag ggcaggcaga ggtgatgcgc ctgatcgacc gtttgggcac
    10321 catcgtctcc accgccgtcg atatccgcga cgacatcatc cgctcctccg aaaagctcat
    10381 gacggagatg atcctcatga tcgcccgcaa ggtgatcaag gacgagatcg tggagcgccg
    10441 cgaggtcgtc atcaacaaca tcaaggaagc catcaagcgc gtgaaggacc gcgaccgcat
    10501 cgacatccgc gtgaacttcg cggacctgga catgaccacg gcgcacaagg acgagctcat
    10561 caagctcatg gagtcgctca agaaggtcaa catctacgag gactcgcgcg tggagcgcgg
    10621 cggctgcatc atcgagaccg acgtgggcgc catcgacgcg cgcatctcga cgcagctcga
    10681 taccatcgag gaggcgatcc ggaacaccag cacggtgtaa ccacctcccg ggccgagcgt
    10741 tttctggaca tcctgtccgc gctcggcact gcaatatcct gttgggagct tcctccttcg
    10801 ttcgggagtg gaggggtgcc ggtggttgga tggccttggc aggagatagg ggaaaaggaa
    10861 cgacggtatt ttttttttat aactaaatga gacataatat atgatcagga tactcccaca
    10921 cgacaaggtc gatatcctct cgaagtacaa gaagatcatc gacgagaccg aactcctcaa
    10981 gtttaccggc tacgtggagc gcgtggtggg gctcacggtg gagtcggtgg gaccgtatgt
    11041 ccaatacggc gagatctgta agataaagct gcgcgacaag cgctatctcc ccgccgaggt
    11101 ggtgggcttc cacaagaacc gcgtggtgct catgcccatc ggcgagatgt ccggcgtgat
    11161 gcccggggcc gaggtgatcg ccatggggag cgccctcatg gtgcccgtgg gggaggagct
    11221 cctcgggcgc gtggtgaacg gcgttggcga gccgctggac ggaaagggcc gcatattcac
    11281 gaaggcgaag tatccggtcg acggcgcggc gccgaatcca atgaaacgaa gcatcatcga
    11341 ccggccgctc tctgtcggga tccgcgccat cgacggcatg ctcacggtgg ggcgcggaca
    11401 gcggatcggt atattctcgg ggtccggcgt gggcaagtcc acgctcctgg cgatgatcgc
    11461 gcggtatacc gacgccgact acaacgtgat cgcgctcatc ggcgagcggg gccgcgaggt
    11521 gcgcgatttc atcgaaaagg agctcgggac cgagggcctt gcgcgctcgg tcgtgatcgt
    11581 cgcaacctcg gaccaaccgc ccatggtgcg gctgcgcggg gcgtttctcg cccatgcggt
    11641 ggcggagtat ttccgcgatc tgggcaagga cgtgaacatc ctcatggact cggtcacccg
    11701 cttcgcacat gcgcagcgcg aggtcggtct ggcgtccggt gagcccgcct cgacgcgcgg
    11761 ttacacgccg tcggtgtaca aaatccttcc ccggctcctc gagcgttcgg ggacgcgcga
    11821 gggcgggggc agcatcaccg ggttctacac gatcctcgtc gaggccgacg atatgaacga
    11881 acccatcgcc gacgcggtgc gcggcatcct cgacggccac gtcgtgctcg atcgcaacct
    11941 cgcgcacaaa gggcactatc cggccatcga cgtgttggga tccatctcgc gctgtatgaa
    12001 ggacgtggtg gacgacgacc acaaggcggc ggcggcgcgt ttccgcgaac tcctcgccgc
    12061 ctttcgcgac aaggaggatc tcatcctcct caatgcctat gcgcgcgggg ccgatcccgt
    12121 ggtggacaag gccatcgaga tgatgggcga cctgaacgcc tttttacggc agggcattca
    12181 cgagcgcgac gaatacgaaa cgatccgcgc tcggttgatt ggcttgttcc ggggagagga
    12241 gaagccggcg gcacgggcag acgcggtcac gccggcctac tatcagcgat tccgcgcggg
    12301 ggcctaggag gaaatcacgt gaaggtgata ctcattcttt ttgtgctcct acccgtttcg
    12361 tgcagtgttg cctccgcatt cggggatctc tcggccgacg agcggaacat gttcgtgacg
    12421 gtcgaggatg tcagggagct gggcgtggct gtaaaaggtt cctcggagaa ggccaaggtc
    12481 gacaagttcg ttttcgcacg ctcgttgacc tggcttggcg gttttgtttt gtatgaatac
    12541 gagtacgacg gtaaggaaaa atcgtcgctg tacatctata actccatcaa caagctgaat
    12601 gcaatgagct ccttcacggg gaagtatttt aagaaggccg ggtttgcggc ggctttcagc
    12661 aaaaaaggca tcgttctcaa ggagttgggc gaattgaaac agcggtatcc caatttctcg
    12721 gtatcgatca tactcaatga ggggaaaccc attggcaacc tcgtgagcgc caatgacgga
    12781 acaatcgatt acaccctgat cttaaccggc gtttttttgg atgagcccga gatctgggcg
    12841 gagctgattg acaagaaaac cgcagcgatg aatgcggtga tgcgatagtg cggcagctgg
    12901 catgaaaaaa ttcgttttca agctcgaaac gctgctggcc atcaaggcgg cgcgcgagaa
    12961 ggagatccag aacgagcttg ccgcgttggt gagcatacag aatgcggagc ggatgcggca
    13021 ggacgaatat cgcgccaaga tcgcccggga gcaggagtcc ttcaacgcga agctgcgctc
    13081 gggacgtttc gcctacgccg acgccatgat gtacgagcgg ttcgtcgagt tcgcgggtaa
    13141 ggtgatcgtc gcggcgcagg ggaagatcga ggcgatggag cccgagatcc agaaggtgcg
    13201 cgaccgactc gtccaggcct ccaccgagcg ccgggtgatc gagaagctca aggagcgcca
    13261 gtggaaggcg caccggtacc aggtcgaggt ggagcttgcc aaggaaaacg acgacgcgaa
    13321 ccagaaggtg cacctgcgca ggaagatcga gatggagcgg gagatacggt catgatggaa
    13381 gacatgtttt cggtgatggg gcggatcgtc gagatcaaga agcgcttcgg actcatgaat
    13441 catccgtata cgcgccagcg gggggccgac ttcagcgagc aggttcggcg tcgtctcgat
    13501 gaggccgatg cgccgcgcag ggacgagcag gccacggtcg atgtcgccgc cggaaaggat
    13561 atgacggttc ccgagatgaa gaagctcgcg caggtgtacg ccggacagaa gcgcgtgccg
    13621 ccatcgctcg tcgacgccgt tatccaggtg gagtcgaatt acaatcccca ggccgtgtct
    13681 cccaaggggg cgctgggact catgcaactc atgccggaca ccgtcggttc gctggggatc
    13741 tccgacccgt tcgatcccgc cgagaacatc cggggcggcg tgtccatcct gcagcggctg
    13801 ctgcagaagt acggcggcga ctacaccaag gcgcttgccg cctacaatgc cggcgagcgg
    13861 cgcgtcgatg agtcgaacgg ggtccccaac atcagggaga cgaaagacta cgtggagaag
    13921 gtaatcaacg cctatctcaa gaacatgaaa taatcggggg cgggatcatg cgcgcgatat
    13981 cggacagggc caagctgctg tacattctct tgctaattat gtttatcgtg gtgatcggct
    14041 ttttctggct ggaccatatc ggtttcatca atctgaagaa gatgttcggc gtcaagaagg
    14101 acgcggtttc cgtgttggac gccaagggcg acgagccgtc gctggtggag cgggaggagt
    14161 tcgagaagga aaagctcaag gtgggggagc gcgtcgagga cctcgacaag cgcgaggccg
    14221 ccatccagga ggccgagaaa accttgcata cggaccgcga gaagctcgag gagatcagaa
    14281 agggccttga gctcgagaag aacaagatcg agaaggaaaa gaagcaatat tccggctaca
    14341 agcgcaatgt ggtggacctc gcccagaaga tcgagaacat cccccccgag caggcggtcg
    14401 agatcctgat ccgatgggag gacacgctgg tcatcgacgt gctgcgccag atcgacttta
    14461 acgcgaccga ggccggcaag atgagcatca gctcgtatct gatatcgctc atgcccaagg
    14521 acaaggccag ccgcatcatg tatctgatga cgcagttgtg atgatttttt tcgaaacggt
    14581 gtaacctttt tccgattgga gctacttacc tatcaacagt ggtcgctcgc cgtaagctcc
    14641 tccagtttgt gttcgacaac cgctgggaag atgattcatc ttctactact ccgattgaat
    14701 tgataccccc ttcatggccg ggccgaatgc ccggctttct ttttttttca accgaccgat
    14761 ccgacgcaaa aacctcaagg gatgccagcg catggccgaa actagtaggg acgagcgatg
    14821 agcctcatcg gcaacaggga gaagcgccat gaaatgcatc ctgaacaatg aattcgtacc
    14881 ctatatcaac aatggaacgc tcaatgtatt ccagatccgc tgtctggtcg agatcaagga
    14941 gttcgtcgat cggatcgccg ggcgccagta cctcgacgga cggaccgttg cgtcgcttac
    15001 cgagaaatac ggggctacgc ccacggtcac gacctgggga gactatttcc agaccgaaat
    15061 cggttttcag ctgctcggga aatcggacga ggaattccgc caggccgtcg acacggtgaa
    15121 gttcgacgtg atctcgagcc atatcatctt caccgggaag gggatcgatt tcttcgagtg
    15181 ggtggagaac aactaccagg actacattaa tcggaaggac gaactgagct tcagggagta
    15241 cgaggagatc gttcatctga agatcctcaa ggattatttc gtcaacctca acatcaccga
    15301 cgcctttacc tacgacgaga tgaagtggta cggcggatac cgcgaggcgg cggccatcta
    15361 acgccgccgc gcgccgtacc gctgctccgc accgcctttc ggacaaaaaa aacgccgttc
    15421 agcccggtta gttcttgacg gatgcaaccg aatcggtttt ggcttgcaaa actgacatcg
    15481 catcattcac tcaggaggag ccatggcttt tcggaaatta tgtacgattt
//
LOCUS       DAORHF010000066        15453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2904_length_15453_cov_6.002728, whole genome shotgun sequence.
ACCESSION   DAORHF010000066 DAORHF010000000
VERSION     DAORHF010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15453
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2904_length_15453_cov_6.002728"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2419)
                     /locus_tag="PKJ16_07540"
     CDS             complement(<1..2419)
                     /locus_tag="PKJ16_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46880.1"
                     /translation="MVRANCAVARSTSWGLVLVVLCALSAAFVSCKADVTPSEKAAKA
                     RALEETQDLYEMLDGYPALASMFRNSNLSVRDFEKRFFDAFLGDALTADNLIVLLDRF
                     PSLFLPREVADDLFPSGVRDEMSEKGPLQDAFFATTETMQNIMSLPPEDKQVIYDFLD
                     MLSGKTVNREFMNDTVAAFLRLAANSLGYCAASVDEDGKRELIEKLIALLDHIIRIND
                     PDSLVTNEEVFAGMRSWAYEFIAYYAAADRDRVNADIAPLLSDLSSMKARRPGELDFV
                     DIDEFINRNVSVSGASAYLFNLADMTRYENVITVSAWLTRMSEAERVRESFRPVLMAL
                     GAFMRQDESRAALKQVLASLRDRYGNGRDGNRALADLLVRVWSEGQEVGDWVRTKNGS
                     PYPGYGQSQNLSIAHLLTQPQLVNSMLVWINRLKGQGYRIDGVGQGFADAITHDPQVQ
                     PRDSGRTYPFPGSFEYQNFSWFRGLLKLASRFSQPLTPASSFLYEHEDGERNWINEGG
                     FNRLVEFFVDAHKIPMASFVWSDVYERNDPDPRGLPGAHPKRTNEDNRYGVFIDEIGD
                     DGRPNGQRRFIAPVFPELITGTEVAFYAGADCLFNGPYDNYYENGNWLFYERNFYCVI
                     DLIQFLPLLHMDVPQPDLKRDGLVKFMVFSMMRALFGSEVNYGEFAAKAFRHFLRVES
                     IPIVLYRFKGVNQIIYSDLGDILRNVGCNIRHGIKAFLRGGTYSFNVGDATVQLAQQR
                     GELSERYRCDFVGDSAAKIAQLQACESRCGGIDCTDGGACPEGESDCEHARECRQCAE
                     CASMDPDL"
     gene            2786..3001
                     /locus_tag="PKJ16_07545"
     CDS             2786..3001
                     /locus_tag="PKJ16_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46881.1"
                     /translation="MDFIKDKIPSIGAFLAIAGILSSVLSFIGYNLRLLLWIDMWGPV
                     VGWVIRIALIVVGGALFFFGKKLVASE"
     gene            complement(3185..3652)
                     /locus_tag="PKJ16_07550"
     CDS             complement(3185..3652)
                     /locus_tag="PKJ16_07550"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV46882.1"
                     /translation="MKVLCSFMGGMVCLAVSIQLFFYGFTEPVANQLWREKSRPATGT
                     VIELKTIREGAISARYPVVEFFTEDGARHTFISKLRSTDIERFTINQTLPVRYSLHDP
                     QNAVIDTSLRTWAPMAVFIAVGGIALAASAVLFVLGMRWHVRSRASTGSREAT"
     gene            3794..3866
                     /locus_tag="PKJ16_07555"
     tRNA            3794..3866
                     /locus_tag="PKJ16_07555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3827..3829,aa:Lys,seq:ctt)
     gene            3929..4009
                     /locus_tag="PKJ16_07560"
     tRNA            3929..4009
                     /locus_tag="PKJ16_07560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3963..3965,aa:Leu,seq:tag)
     gene            4014..4085
                     /locus_tag="PKJ16_07565"
     tRNA            4014..4085
                     /locus_tag="PKJ16_07565"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4046..4048,aa:Gly,seq:gcc)
     gene            4157..5629
                     /locus_tag="PKJ16_07570"
     CDS             4157..5629
                     /locus_tag="PKJ16_07570"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HNV46883.1"
                     /translation="MKLSVRTHPDEFDAQDTIVLFLSDEFNAAASQEIPPSLSDLRTR
                     IDFGFFRGRPGDLIFLPFSERPAVILAGIGKRKDLSAESLRNAAAKVIASCAKHRIAR
                     VMMIAPAVDALPPADVIAALAEGAYLSNYSFKKYKSKKDNDFTVGEAVFFHKNPNELR
                     TRLREIEIACDNTLRCRDLVNETSDRSNPVEIAKLAKSLARGRRLSCQVLGKKEIERL
                     GMGLLLAVSRGSSYPPQLVVFKYAGNPRSKKCIALVGKGITFDSGGLNLKPSGHIEDM
                     RTDMAGAAACLYAVKSAAELGIKKNVVAVVPLCENMLSSNSFRPGDVYRAYNGKTVEI
                     GNTDAEGRLILADALAYTEERFTPSYIIDAATLTGACLVCFGEIIAALLTGDDALAQA
                     IATAGDITGDRVWRLPLDKGYDDDIKSDIADMNNISSGRQAGTIIGAVFMKNFVKSAP
                     WAHLDIAGTSWYTKERGYRPKYATGYGVRLFLELVKRLEP"
     gene            complement(5636..6253)
                     /locus_tag="PKJ16_07575"
     CDS             complement(5636..6253)
                     /locus_tag="PKJ16_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV46884.1"
                     /translation="MIRRPHASEAGQCSALLYLSGPPVFDYFFASAEPDVCRYLSMFF
                     NKPDTAFSREYIWVEENDGAIRGVLLALPGSDKTRLERNMGAHGRELVRMAGFFRSMR
                     MLLHGRLGKYLAVIDADEYYIGNLAVRAEHRGMGVGPALLRKAEELAGEQGYRKLSLL
                     VEVNNRHARHVYDAFGFSTVRELMLPQRYNRYGLIGFYKVVKALS"
     gene            complement(6320..9136)
                     /gene="rapA"
                     /locus_tag="PKJ16_07580"
     CDS             complement(6320..9136)
                     /gene="rapA"
                     /locus_tag="PKJ16_07580"
                     /inference="COORDINATES: protein motif:HMM:NF003426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HNV46885.1"
                     /translation="MGAFKVGQRWISEMELELGLGTVRAVRARTVEMHFAASDCTRVY
                     AVATAPLRRAAFMAGDTVRPRGGAPFEVKAVSEEAGLRVYHGDAIDCAESDLDDAISF
                     TTPRIRLFGKLFDHDRDFRIRYRALSLQRRIVQSPVRGFLGGRISLIPHQLYVTHAVA
                     ERAVPRVLLSDEIGLGKTIEACLVLQRLLLCRRIDRVLILVPQSLVHQWFAELLRRFN
                     LVFKIYNRDYQKSVRASDRRRNPFHEDQRFIAAVEFVAADATARAMCAEAGWDVVVVD
                     EAHHLVEGGPEYSLVDSLGAASPGLILLTATPEQFGRRSHFARMRLLDPARYHDYERF
                     DREEERYYAISRIANMVMHGEVPDDEALSVLGPGAARISAELRAGVAAADDAARQRFI
                     GDLVDRYGTGRAIFRNTREGMTGFPERRVRLVPLAGTEAVQKNMTDEFAADIADGAIA
                     IDHDFSADPRCAWLGDFLKEHPDRKALLLCRTREKAVAIEKFLQRRGHADIALFHEDL
                     PLLVRDQNAAWFAMARGARLLISSEIGSEGRNFQFAHDLILFDLPTDPELLEQRIGRL
                     DRIGQGAAIDIHVPFVIGSEYEVLARWYHEGADAFATIVPGAYELYQRFGEAIVSVAQ
                     RRDHGALAALVPETRAFCAEIAERLRNGRDRLLELNSFDPAAAERLLGEIRDIERDGE
                     LEAFMLDIFEHFNIVAEPLSDRTHRLRLDLMTTDEFPLPVIGRDDLRVTFSRESALRN
                     EDLEFLTWDHPMVTGVLDIVLGRERGNCALGAWHDPSTPELLLEAVFVVECVAPRRLH
                     VDRFLPPRPIRVVVNNANRDRTDAITVDLCERNVEHYADMEVLDDDDVKNVLLPRMIA
                     RCVQIAEEGVAGIVAAGLSSLDITLGNDLARMKALGAINTNITEDEIAVCGREIDALR
                     EAIRSARVRLDSLRLILKGDLESD"
     gene            complement(9393..10358)
                     /locus_tag="PKJ16_07585"
     CDS             complement(9393..10358)
                     /locus_tag="PKJ16_07585"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV46886.1"
                     /translation="MAPDSIEYYSRDADYILREALTNMKGLIDFLYFGGLEECSEEEN
                     ARFHVKLKESSDTLYSLLNSNIIEKANRIEKLNAIIRMDLELASKIQHKILPDNSGTI
                     GGMDFHVSFIPMMQVGGDIYDIAEIRPGCVRIFLADATGHGVQTALITMIIKSEYEML
                     KNVIASPAELLVTLNDMFTRVYSGLGMFFTCIIVDVDVREKTMRYSSAGHPVQYLIRP
                     SGEFVPLSHKGVLVGFREGYRYREDVMAVPDGGKLMLYSDGIYEEFNAVKDEYGEERL
                     RSFLERCTHRDVRDMVEAVVDDLMGFIGGQELSDDITMIGVELPK"
     gene            complement(10427..10864)
                     /locus_tag="PKJ16_07590"
     CDS             complement(10427..10864)
                     /locus_tag="PKJ16_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46887.1"
                     /translation="MPKVLIVDDKEENRYVLKNFFKLFGVNSGIEIVEAVSGEDAVAK
                     ARADKPDLIIMDIKMETDYAGLDATRTIRSDEVVNNTPIWALTSQAMEAHDGDEGDRD
                     KCLNAGCNDYITKPFDQIELIRKISGLLNIEIPKKTKVRMGIE"
     gene            complement(10908..14267)
                     /locus_tag="PKJ16_07595"
     CDS             complement(10908..14267)
                     /locus_tag="PKJ16_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46888.1"
                     /translation="MNRVKAALSGTIVGLVFIALMYSSSYAVEPIRLTERTLSGSPIG
                     RHVEYCEDTTGALSLQSLLENTARGTVAWKASAHESLGFGFTNSAYWIRFTVVNERSE
                     PLEWYFDTGYPLNREVDLYVPRGGGFILKQEGVRYVYGEREFDHHNIIFPLHESPGSH
                     TYYLRVRSHGSITLPLRAWSRGELYSNSFLVYMVQGIFLGMLVILALSNLLIFALSRD
                     VNYIFSALFYSAFVMASLTFTGIGYRLFWPDTPWFNYGIAFWIFMTLVFASLLTRSYL
                     GLQERSARLNRLVIAMAVLYFTGMALSWAAPYRIIVSLVAVSILVSLAVFLFVGIVLL
                     VRRVREAYFHTLAWIVMLAFCSLTALKSLGVVPGGFIATYGFEIGVTSGAFLFSLGLS
                     DRINTLKNALATLNADLEQTVDERTEALHRSEAKYSSILEVSKIGYYEVDINGAFLDV
                     NRPLAEFLGYTVDELIGMNYARLVDEKNALGVYEFFNRIHRREMDRGMIEIEVIARDG
                     SRKCGDNYVSRIENDAGGTIGFRAVTIDASDRREAEDELRRSEEKYRHFIENANEVIY
                     KSDWRGNFLFANEAFSRLLGYTRDEILQMNYLDVIPPERRDEEFAFYKRQLQGRIPAS
                     FRELPVVAKTGAVVWVEQNVKSVFGDDGRIVEFDAIVRDITERKIAQDALRDIEQGYR
                     TILDNVADAIGVCDFSGTILYMNSSGWRMTGYEQGESLNFLDIIHPDQREALAASFIR
                     QQMENIPFIRNEFLMVRKDGTSLWCDHISTLTRDAAGKRILCGIGRDISESRRAKQEL
                     EDAKEAAEAASLAKSRFLANMSHELRTPLNAINGIIDLLQYGSFERDEEILAALGGLA
                     QRLRDLPDGDARDQAERLRTALDSLMRHLADDGNCKRYILGGIRGDFRALRIDDDPAV
                     AALFGDIDRYIADEETAAQSSYRRIKESGEYLLGLIDMVLNLAKIETGNIEIVKSEVR
                     LQEMIGSVLRNAQSYAASKGKAHVAIVTELEDGVPDTMVLDNQKTHQALLNYLSNAVK
                     FTEAGSVTLAVSRIGGRVRFAVRDTGIGIRDEDRVKIFVEFERTESARYIEGTGLGLA
                     ITKRLIELQGGAVGFESQYGTGSVFWFELPV"
     gene            complement(14305..>15453)
                     /locus_tag="PKJ16_07600"
     CDS             complement(14305..>15453)
                     /locus_tag="PKJ16_07600"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46889.1"
                     /translation="FKRQYEEKIDETYLEFPVLRGDGRMIWLGQRVRLEKAPTGTCEY
                     TGISRDITERVRADEELRRTKDAAEVANRTKSRFLASMSHELRTPLNSINGVIDMLRF
                     GSHACDEEVLGKLRSLARLLREEESRCGTIDSIALQALLDSLIGLYSNEGNGHRCVPG
                     VLEVGIRSSACAASTEAKALVDDIAALIDAEENQTRDAYQRVKDASLHLLEIIDSVLN
                     LAMIESGSEAVHKTTSRTREVVDSAVTNARSSARSMGKEHLRITSVIREDVPDIMRLD
                     RYKIGQVLMNLFSNAIKFTDDGDIDFEVRRDGDRVRFSIRDSGIGIREKDRERVFEDF
                     ERAEDARNFKGTGLGLAISKRLVELHGGDVGFESEYGKGSTFWFSLPL"
BASE COUNT         3018 a         4968 c         4552 g         2915 t
ORIGIN      
        1 tcaggtccgg gtccatcgac gcgcactcgg cgcattgccg gcactcgcgc gcatgctcgc
       61 agtccgattc gccctccggg catgcgccgc cgtccgtgca gtcgatgccg ccgcaccggc
      121 tctcgcaggc ctgcagctgc gcgatcttcg cggcgctgtc gcccacgaaa tcgcagcgat
      181 accgctcgga gagctcgccg cgctgctgtg cgagctgcac ggtggcgtcc cccacgttga
      241 agctgtaggt cccgccccgg aggaaggcct tgatcccgtg cctgatattg cagccgacgt
      301 tccggagaat gtcgccgaga tcggagtaga tgatctgatt cacgcccttg aagcggtaca
      361 ggacgatggg aatgctctcc acgcggagga agtgccggaa ggccttggcg gcgaactcgc
      421 cgtagttcac ctcggagccg aacaacgcgc gcatcatcga aaagaccatg aacttcacca
      481 ggccgtcccg cttcaggtcc ggctgcggga cgtccatgtg caggagcggc aaaaactgga
      541 tgaggtcgat gacgcaatag aagttccgct cgtagaagag ccagtttccg ttctcgtagt
      601 aattgtcgta ggggccgttg aacaggcagt cggccccggc atagaaggcc acctcggtcc
      661 cggtgatcag ctccgggaac acgggtgcga tgaaacggcg ctgcccgttc ggacgcccat
      721 cgtccccgat ctcgtcgatg aatacgccgt agcgattgtc ctcgttggtg cgcttcgggt
      781 gcgcgccggg caggccgcgc ggatcgggat cgttgcgctc gtacacgtcg gaccacacga
      841 agctcgccat cgggatcttg tgcgcgtcca cgaagaactc caccagcctg ttaaacccgc
      901 cctcgttgat ccagttgcgc tcgccgtcct cgtgctcgta gagaaagctc gacgccggcg
      961 tgagcggctg cgagaagcgc gaggcgagtt tcagcaggcc ccggaaccag gagaagtttt
     1021 gatactcgaa cgatcccggg aacgggtagg tccgtccgct gtcgcgcggc tgcacctgcg
     1081 ggtcatgggt gatggcgtcg gcgaacccct gccccacccc gtcgatgcgg tacccctggc
     1141 ccttgagccg gttgatccac acgagcatcg agttgaccag ctgcggttgc gtgagcagat
     1201 gcgcgatcga aagattctgc gattgcccgt accccgggta cggcgagccg ttcttggtgc
     1261 gcacccagtc gcccacctcc tgcccctcgg accagacccg gacgagcagg tccgccagcg
     1321 cccggttccc gtcccgcccg ttgccgtagc ggtcgcgcaa cgatgcgagc acctgcttga
     1381 gcgccgcgcg cgactcgtct tgccgcatga aggcgccgag cgccatgagc accggtcgga
     1441 acgactcgcg cacccgctcg gcctcgctca tccgcgtcag ccaggcgcta acggtgatga
     1501 cgttctcgta ccgcgtcatg tccgcgagat tgaacagata cgccgacgcg ccggacacgc
     1561 tcacgttccg gttgatgaat tcgtcgatgt ccacgaagtc gagctcgccg ggacggcgcg
     1621 ccttcatgga cgaaaggtcc gacaggagcg gcgcgatatc ggcgttcacg cgatcgcggt
     1681 cggccgccgc gtagtaggcg atgaactcgt acgcccagga gcgcatcccc gcgaacacct
     1741 cctcgttggt cacaagggaa tcgggatcgt ttatgcggat gatgtgatcg aggagcgcga
     1801 tgagcttctc gatcagctcc cgctttccgt cctcatcgac actggccgcg caatagccca
     1861 gggaattcgc ggcgagccgc agaaacgccg ccacggtgtc gttcatgaac tcgcggttca
     1921 cggtcttgcc ggagagcatg tcgaggaagt cgtagatgac ctgcttgtcc tccggcggga
     1981 ggctcatgat gttttgcatc gtctcggtgg tcgcgaagaa cgcgtcctgc agcggtccct
     2041 tctcggacat ctcgtcccgc acgcccgatg gaaacaggtc atcggcaacc tcccgcggga
     2101 gaaacagcga cgggaaccgg tcgagcagca cgatgaggtt gtccgccgtg agcgcatcgc
     2161 ccaggaaggc atcgaagaag cgcttctcga agtcccgcac cgacaggtta gaattgcgga
     2221 acatcgaggc aagcgcggga tacccgtcca gcatctcgta gaggtcctgc gtctcctcga
     2281 gcgcgcgcgc cttggcggcc ttctccgatg gagtgacatc ggccttgcag gacacaaacg
     2341 cagcgctcag agcgcacagg accacgagaa caagacccca cgaagtggat cgtgcaacgg
     2401 cgcaattcgc tctcaccata cccatcatca tccgttcctc gctgtgtgtt ttggctttct
     2461 ctttctgttt cttctaacgt caaaaacgtt cgaacgcggc gtcgcttcat cgcaaaccga
     2521 ccgaccgttc ggcgatgttc gccgtatttt cccagcttcg tgatgatgaa tatgagggga
     2581 aaaacctttc gggttccaat caataaccgg gttaacaaaa attgaacatg cggtatcgat
     2641 gatttccgat catgcgcatg cttcattcag ctgcgggcgg tcgcgaatgg atattcgatg
     2701 cggtgtggac gaaacaagtt cttgacatcg tgcgatcaat atgcaccact atgccataga
     2761 acgcatacac acaatggagg ttgcaatgga tttcattaag gacaaaatcc cgtcaatcgg
     2821 cgcatttctc gccatcgcgg gcatcctgtc gagcgtgctg agttttatcg gctacaacct
     2881 gcgtcttctg ctctggatcg acatgtgggg accggtggtc ggctgggtca tccgtatcgc
     2941 gctcatcgtc gtgggcggcg cgctcttctt cttcgggaaa aagttggtcg ccagcgagta
     3001 gcgaacacgc gcatcacctc gaatcgaaac ggccggaatc ccgcgcgcgg catggcgggg
     3061 cgccggccgt ttcattatcg cgcatacgga tatcgagagg cgccaggcgc attctccgcg
     3121 atcgcgccga tcatcccgac gagcacggaa tgcacgcccg caacccggcg acaggcggag
     3181 tcgatcacgt tgcttcgcgc gaaccggtcg aggctcgcga tcggacgtgc cagcgcatgc
     3241 caaggacgaa gaggaccgcg cttgcggcca gggcgatgcc gcccacagcg atgaagacgg
     3301 ccatgggcgc ccaggtgcgc agcgaggtgt cgatgacggc attctgcgga tcatgcaggc
     3361 tgtagcggac gggcagcgtc tggttgatgg taaagcgctc gatgtcggtc gagcggagct
     3421 tgctgatgaa ggtgtgacgg gcgccgtctt cggtaaagaa ttcgacgacc ggatagcgcg
     3481 ccgaaatggc gccctcgcgg atcgttttca gctcgatcac cgtgccggtc gcgggccgcg
     3541 atttctcacg ccagagctgg ttggccaccg gctccgtaaa cccgtagaaa aacagctgga
     3601 tggaaacggc gaggcagacc atcccgccca tgaacgaaca caataccttc atagcgatgc
     3661 tccgctcgaa ttggatcgca acgcacgcaa ggtgtcaatc ccaattccaa caccccgcac
     3721 tgcagatgcg cggcggtggc gaataaaaaa acttgacaca ttttcgcacc gttaactatg
     3781 gttacatctg cttgggttgc tagctcaaca ggcagagcaa ctgactctta atcagtaggt
     3841 ttagggttcg agtccctggc aacccacatc gccgccccgt aaatttagct tgattttggg
     3901 gatgccaaaa ggtaattgac aaattcaagc gagcgtggcg gaattggtag acgcgccaga
     3961 tttaggatct ggtgtctccg acgtgggggt tcgagtccct tcgctcgcaa taggcgggaa
     4021 taactcagcg gtagagtgtc accttgccaa ggtgaaggtc gcgggttcaa atcccgtttc
     4081 ccgctcatca gaaagggttg ccgaggacac acatcggcaa ccctttcgtt tatctccatc
     4141 cccgaggaca tgacccatga agctatcggt ccgtacgcac cccgacgagt tcgacgcaca
     4201 ggacaccatc gtcctcttcc tgtcggacga attcaatgcc gccgcatcgc aggagatccc
     4261 gccatcgctt tcggacctgc gtacgcgcat cgatttcggc tttttccggg gacgcccggg
     4321 cgatctcatt ttcctgccct tcagcgagcg gcccgccgtg atcctggcgg ggatcggaaa
     4381 gcggaaggat ctgagcgccg agtcgctccg caatgccgcc gccaaggtga tcgccagctg
     4441 cgcgaaacac cggatcgccc gggtcatgat gatcgcgccc gccgtcgatg cgctcccgcc
     4501 ggccgacgtt atcgctgcgc tcgcggaggg cgcgtatcta tcgaactact cctttaaaaa
     4561 atacaagtcg aagaaggaca acgacttcac ggtcggcgaa gcggtcttct ttcacaaaaa
     4621 cccgaacgag ctgcgcacgc gcctgcgcga aatcgaaatc gcctgcgaca acacactccg
     4681 ctgccgcgac ctggtgaacg agaccagcga ccgctccaac cccgtcgaga tcgcgaagct
     4741 cgcgaagtcg ctcgccaggg gacggcgcct ctcgtgccag gtgctcggaa aaaaggagat
     4801 cgaacggctc ggcatggggc tgctcttggc cgtgagccgc gggagctcgt atccgccgca
     4861 gctggtggta ttcaaatacg ccggcaaccc gcgcagtaaa aaatgcatcg ccctggtggg
     4921 caaggggatc accttcgact cggggggctt aaacctcaag ccgtcgggcc acatcgagga
     4981 catgcgcacc gacatggccg gcgcggccgc ctgcctctac gccgtgaaga gcgccgcgga
     5041 gctgggcatc aagaaaaacg tcgtggccgt ggtgccgctg tgcgagaaca tgctctccag
     5101 caattccttc cgtcccggcg acgtgtatcg cgcgtacaac ggcaagaccg ttgagatcgg
     5161 gaacaccgac gccgaggggc gcctcatcct ggccgacgcg ctcgcctaca cggaggagcg
     5221 cttcacgccg tcgtatatca tcgacgccgc cacgctcacc ggggcctgcc tggtgtgctt
     5281 cggcgagatc atcgccgcgc tcttgaccgg cgacgatgcg ctcgcgcagg cgatcgcgac
     5341 cgcgggcgat atcacgggcg accgcgtctg gcgcctgccg ctggacaagg gctacgacga
     5401 cgacatcaag tccgacatcg ccgacatgaa caatatctcc tcgggacggc aggcggggac
     5461 catcatcggc gccgtgttca tgaagaattt cgtgaagagc gcgccgtggg cgcacctgga
     5521 catcgcgggg acctcgtggt acaccaagga gcgcggctat cggcccaagt atgcgaccgg
     5581 gtatggcgtg cgcttgttcc tggagctcgt caagcgcctg gagccgtgag cgggatcagg
     5641 acagcgcctt gacgaccttg tagaacccga tgagcccata ccgattgtag cgctggggca
     5701 gcatcagctc gcgcaccgtc gaaaaaccga aggcatcgta cacgtgccgg gcgtgccggt
     5761 tgttcacctc gaccaggagc gacaattttc gatacccctg ctcccccgcc agctcctcag
     5821 ccttccgcag cagcgccggc ccgacgccca ttccgcgatg ctcggcgcgc acggccaggt
     5881 ttccgatgta atattcgtcc gcatcgatca ccgcgaggta tttccccagg cgtccgtgca
     5941 gcaacatgcg catgctccgg aaaaagccgg ccatccgcac cagctcacgc ccatgggcgc
     6001 ccatgttccg ttcgagccgc gtcttgtccg atccgggcag cgcaagcagc accccgcgga
     6061 tcgccccgtc gttttcctcg acccagatgt actcacgcga gaaggccgtg tcgggcttgt
     6121 tgaaaaacat ggaaagatag cggcacacgt ccggctccgc cgatgcgaag aagtagtcga
     6181 acaccggagg gcccgacaga tacagcaggg cgctgcactg gccggcctcg gatgcatgcg
     6241 gtcgtctgat catatcgccc ctccacccat atccactgtg acgcgtgcga ggcgagcatg
     6301 cggacgacgg aggatcgcgt caatcgcttt ccaggtcgcc cttcagtatg aggcgcagcg
     6361 aatcgagccg tacgcgcgcg gagcggatgg cctcccggag cgcatcgatc tcccggccgc
     6421 acacggcgat ctcatcctcc gtgatgtttg tgttgatggc gccgagcgct ttcatgcgcg
     6481 caaggtcgtt gccgagcgtg atatcgaggg acgacaggcc cgccgcgacg atgcccgcaa
     6541 cgccctcctc ggcgatctgc acgcaccggg cgatcatacg cggcaacagc acgttcttca
     6601 cgtcgtcatc gtccagcacc tccatgtccg catagtgctc gacgttccgc tcgcagaggt
     6661 cgacggtaat agcgtccgtg cggtcccgat tcgcattgtt gaccacgacg cggatcggcc
     6721 gcggcggcag gaagcggtcc acgtgcagcc gacgcggcgc gacgcactcc acgacgaaca
     6781 cggcctcaag cagaagctcc ggtgtggacg gatcatgcca tgccccgagc gcgcaatttc
     6841 cccgctcgcg ccccaggacg atgtccagga cgccggtgac catcggatgg tcccaggtga
     6901 gaaactccag gtcctcgttt cgaagggcgg actcgcgcga gaacgtcacc cgcagatcgt
     6961 cccgcccgat gaccgggagc ggaaactcgt cggtggtcat gaggtccagg cgcaaccgat
     7021 gggtgcggtc cgacagcggc tcggcgacga tgttgaaatg ctcgaagatg tccagcatga
     7081 acgcctccag ctcgccgtcg cgctcgatat cccggatctc gccgagcagc cgctccgcgg
     7141 ccgcgggatc gaacgagttg agctcgagca gccggtcccg tccgttcctg aggcgctcgg
     7201 cgatctcggc gcaaaacgcg cgggtctccg ggacgagcgc cgcgagggcg ccatggtccc
     7261 tgcgttgcgc gacactcaca atcgcctccc cgaagcgctg atagagctcg taggccccgg
     7321 ggacgatcgt ggcgaaggcg tccgcgccct catggtacca gcgcgcgagg acctcgtact
     7381 cgcttccaat cacgaacggg acatggatgt cgatggccgc cccctggccg attcggtcga
     7441 gccgtccgat ccgctgctcg agcagctccg ggtccgtcgg caggtcgaac agaatgagat
     7501 cgtgcgcgaa ctggaagttg cgcccctcgc tgccgatctc ggaggatatc agcaaccgcg
     7561 cgccgcgggc catcgcgaac cacgccgcgt tctggtcccg cacaaggagc ggaaggtcct
     7621 cgtggaacag ggcgatatcg gcatgccccc gacgctgtaa gaatttctcg atcgcgacgg
     7681 ccttttccct ggtgcggcac aacagcagcg ccttgcgatc ggggtgttcc ttgagaaagt
     7741 cgccgagcca cgcgcatcgc ggatcggcgg aaaaatcgtg gtcgattgca atcgccccgt
     7801 cggcgatgtc cgccgcgaac tcgtcggtca tgtttttttg caccgcctcg gttcccgcga
     7861 gcgggaccag gcgcaccctc cgttcgggaa agccggtcat cccctcccgc gtattgcgga
     7921 aaatggcgcg cccggtccca tagcggtcca ccaggtcgcc gatgaaccgc tggcgcgcag
     7981 catcgtccgc agcggcgacg cccgcacgca gctccgcgga gattcgcgcg gcgccggggc
     8041 ccagcaccga aagcgcctcg tcgtccggca cctcgccgtg catcaccatg ttggcgatgc
     8101 gggatatcgc gtagtagcgc tcctcctcgc ggtcgaaccg ttcgtaatcg tggtagcgcg
     8161 cggggtccag caatcgcatg cgggcgaagt ggctgcggcg gccgaactgc tccggcgtcg
     8221 ccgtcaggag gatgagtccc ggcgacgccg cgccgagcga atcgaccagc gaatactccg
     8281 gaccgccctc gacgagatga tgcgcctcgt caacgacaac gacgtcccag ccggcctccg
     8341 cgcacatcgc gcgcgccgtt gcatcggccg caacaaactc gacggcggcg atgaaccgct
     8401 ggtcctcgtg gaacggattg cgccgcctgt ccgaggcgcg gaccgatttc tgatagtcgc
     8461 gattgtagat tttaaacacg aggttaaaac ggcgcaaaag ctccgcgaac cactggtgga
     8521 ccagcgactg gggcaccaga atgagcacac ggtcgatccg ccggcacagg agcaggcgct
     8581 ggaggaccag acaggcctcg atggttttcc ccagcccgat ttcgtccgag agcagcaccc
     8641 ggggaacagc gcgctcggcg accgcgtggg tcacataaag ctggtgcggg atcaggctga
     8701 tccgcccgcc caggaagccc cggaccggcg actggacaat gcgccgttgc agcgagaggg
     8761 cgcggtaccg gatacggaag tcccggtcgt ggtcgaacag cttgccgaaa aggcgtattc
     8821 gcggcgtggt gaagctgatg gcgtcgtcca gatcggattc ggcgcaatcg atcgcgtccc
     8881 catgatagac gcgcaagccc gcctcctcgg agacggcctt gacctcgaag ggtgcgcccc
     8941 cgcggggccg cacggtatcg cccgccatga acgcggcccg ccgcagcggc gccgtggcga
     9001 ccgcatagac gcgggtgcag tcgctcgcgg cgaagtgcat ctccaccgtt cgcgcgcgca
     9061 cggcgcggac ggtcccgagt cccaactcga gctccatctc gctgatccag cgctgtccga
     9121 ctttaaacgc ccccaacggc ttgctcccgc atgctcggaa tcatccgcac atgtccaaca
     9181 ggacaccacg acatggcgga tggtcaccgg tagactgtgg cgcgcaatcc gtcaaggggt
     9241 tcacgagatg gggcaacgat tcggctgcga ggtgcaaccg gccgtgaacg acatccgggc
     9301 atgcgctccg gcgcacgcgt ttgcgcgcag gagcgacgca tgcctccggg gacatcggcc
     9361 ggatcatccg atacgcgata gtaggataag gcctatttcg gcagctctac gccaatcatg
     9421 gtgatgtcgt cgctgagttc ctggccgccg atgaatccca tcagatcgtc cacgacggcc
     9481 tcgaccatgt cacggacatc ccggtgcgtg caacgctcga gaaacgaccg gagccgctcc
     9541 tcgccgtatt cgtccttcac cgcattgaac tcctcgtata tgccgtctga atacagcatc
     9601 agcttgccgc cgtccggcac cgccatcacg tcctcgcgat accgatatcc ctcccggaat
     9661 cccaccagga cccccttgtg cgacagggga acgaactcgc ccgagggccg aatcaggtac
     9721 tgcaccggat gaccggcgct cgaatagcgc atcgtcttct cgcgcacatc gacatcgacg
     9781 atgatgcagg tgaagaacat cccgagaccg ctgtagacgc gggtgaacat gtcgttgagc
     9841 gtcaccagga gctccgcggg acttgcaatg acgttcttga gcatctcgta ttcgctcttg
     9901 atgatcatgg tgatgagcgc cgtctgaacg ccatgaccgg tcgcatcggc gaggaatatc
     9961 cgcacgcacc cgggcctgat ctcggcgata tcgtagatat cgccgcccac ctgcatcatg
    10021 ggaataaagg agacatgaaa gtccatgccg ccgatggtcc cggagttgtc gggaaggatc
    10081 ttgtgctgga tcttgctcgc cagctcgagg tccatccgta tgatcgcgtt gagcttctcg
    10141 atccggttcg ccttctcgat gatattcgag ttgagcaagg aatagagcgt atcggagctc
    10201 tccttgagtt taacgtggaa ccgcgcattc tcctcctcgc tgcattcctc cagcccgccg
    10261 aaatacagga aatcgatgag gcccttcatg ttggtgagcg cctcgcgcag gatgtaatcc
    10321 gcgtcccggg aatagtactc tatgctgtca ggtgccatac gctccctcca tctctactgc
    10381 gtccctccgc attgggtgca acacgcgctc tgtttctccg agacggttac tcgataccca
    10441 ttcggacctt ggtcttcttg ggtatttcga tgttcaacag gcccgatatt tttcgaatga
    10501 gctcgatctg atcgaagggc ttggtgatgt aatcgttgca gcccgcattg aggcacttgt
    10561 cccgatcgcc ctcgtcgccg tcgtgggcct ccatcgcctg cgaggtgagc gcccagatgg
    10621 gcgtgttgtt gaccacctca tcggaacgga tggtgcgcgt cgcgtcgagc ccggcatagt
    10681 ccgtttccat tttaatgtcc atgataatga gatccggctt gtccgcgcgc gccttcgcca
    10741 ccgcgtcctc gcccgagacc gcttcgacga tctcgatccc ggagttgacc ccgaacagct
    10801 tgaaaaaatt cttcaataca taccgattct cttccttgtc gtccacaatg agcactttag
    10861 gcattctatc cctccattca gtaagcgcgg aaaccgcacc ttcgctatta cactggtaac
    10921 tcgaaccaga aaacgctccc cgtcccatac tgcgactcga aaccgacggc cccgccctgc
    10981 agttcaatga gccgcttcgt tatcgccaga cccaatcccg ttccctcgat gtaccgcgca
    11041 ctctccgtac gctcaaactc cacgaaaatc ttaactcggt cctcgtcgcg aatcccgatc
    11101 ccggtgtcgc gaaccgcaaa gcgcacgcgt ccgccgatgc ggcttaccgc gagcgttacc
    11161 gaacccgcct cggtgaactt gacggcgttc gagaggtagt tcaacagcgc ctggtgcgtc
    11221 ttctgattgt cgagcaccat cgtgtccggg acaccgtcct ccagttcggt gacgatcgcg
    11281 acatgcgcct ttcccttgga agcggcatag ctctgcgcat tccgcaacac cgacccgatc
    11341 atctcctgca ggcgcacctc gctcttcaca atctcgatgt tgcccgtttc aatttttgcg
    11401 agattgagca ccatgtcgat cagccccaac aggtattcgc ccgactcctt gatccgccga
    11461 taggatgatt gggccgcggt ttcctcgtcg gcgatatacc ggtcgatgtc gccgaacagg
    11521 gcagcgacgg ccgggtcgtc gtcgatccgc aatgcgcgga agtctccgcg gatcccgccg
    11581 agaatatacc gcttgcaatt accgtcatcg gcgagatgcc gcatgaggga gtccagcgcg
    11641 gttcgcaggc gctccgcctg gtcccgcgca tcgccgtccg gcaggtcccg caggcgttgc
    11701 gccagcccgc cgagcgcggc gaggatctcc tcgtcccgct cgaaggagcc gtactgcagc
    11761 aggtcgatga tgccgttgat cgcgttcagc ggcgtccgga gctcgtggct catgttcgcg
    11821 aggaaccggc ttttcgccag gctcgcggcc tcggcggcct ccttcgcgtc ttcgagctcc
    11881 tgtttcgcgc gccggctctc cgatatgtcg cgcccgatgc cgcacagtat tcgcttgcca
    11941 gcggcgtccc gcgtcagcgt cgagatgtgg tcgcaccaca gggacgtccc gtccttgcgc
    12001 accatgagaa attcattgcg gatgaatgga atgttttcca tctgctggcg gataaaactc
    12061 gccgcaagcg cttcccgttg gtcgggatgg atgatgtcga gaaaattcag cgattcgccc
    12121 tgttcgtagc cggtcatcct ccagccggac gaattcatat agaggattgt tcccgagaaa
    12181 tcgcacacgc cgatggcgtc ggcgacatta tccaggatgg tccgataccc ctgctcgata
    12241 tcgcgcagcg catcctgcgc gattttccgc tcggttatat cgcggacgat tgcgtcgaac
    12301 tcgacgatcc ggccgtcatc gccgaatacc gatttcacgt tttgctccac ccacaccacc
    12361 gcgccggtct ttgcgacaac cggcagctct cggaatgacg cgggaatcct gccctgcagc
    12421 tgtcgtttgt aaaacgcgaa ctcctcatcc cgccgctccg gcgggatcac atcgagataa
    12481 ttcatttgga gtatttcgtc acgggtatac ccgagcagcc tggagaatgc ctcattggcg
    12541 aaaaggaaat tcccccgcca atcggattta tagatcacct cgttcgcgtt ttcaatgaag
    12601 tgccgatatt tctcctcgct gcgacggagc tcgtcctccg cctcccgcct gtcgctggcg
    12661 tcgatcgtga ccgccctgaa accgatcgtc ccgcctgcgt cgttctcgat gcgactcacg
    12721 taattgtccc cgcacttccg cgatccgtcc ctggcgataa cctcgatctc gatcattccg
    12781 cgatccatct ctcggcggtg gatgcggtta aaaaattcgt acacgccgag cgcgtttttc
    12841 tcatccacca ggcgcgcgta attcatgccg atgagctcgt cgaccgtata accgaggaac
    12901 tccgcaaggg gcctgttgac atcaagaaag gctccgttga tatcgacctc gtaatacccg
    12961 attttgctga cctcgaggat ggagctgtac ttcgcctcgg agcgatggag ggcctcggtc
    13021 cgttcgtcaa cggtttgctc gagatcggca ttgagggtcg ccagcgcgtt cttcagcgtg
    13081 ttgatcctgt cggagagtcc cagcgagaaa agaaacgcgc ccgacgtgac gccgatttcg
    13141 aatccgtacg tcgcgataaa tccgccggga accaccccca ggcttttcaa ggcggtcagg
    13201 gaacaaaagg ccagcatcac gatccaggcg agggtgtgaa agtacgcctc ccgcacccgt
    13261 ctgacgagca atacgatacc gacaaataaa aacaccgcca ggctgacgag gatggagacc
    13321 gccaccaacg acacgatgat ccggtagggc gccgcccatg acagcgccat cccggtgaaa
    13381 tacagcacgg ccatggcgat gaccaatcgg ttgagtctcg ccgaacgctc ttgaagcccg
    13441 aggtacgacc gcgtgagcag cgacgcgaac acaagcgtca tgaatatcca gaaagcgatg
    13501 ccgtaattga accacggagt atcgggccag aacaatcggt acccgatgcc ggtaaaggta
    13561 aggctggcca tgacaaatgc ggaatagaac agcgcgctga agatatagtt tacatcgcgc
    13621 gagagcgcga atatcaacag attggaaagc gccaggatga ccaacatgcc caaaaagatc
    13681 ccctggacca tgtacacgag aaatgaattg gaatacagct cacctctcga ccatgcgcga
    13741 agcggaaggg tgatcgagcc gtgcgatcgc acccgcagat agtaggtatg cgatcccggc
    13801 gactcgtgca acgggaaaat gatattgtga tggtcgaact cccgctcccc gtacacatag
    13861 cgcacgccct cctgcttgag gataaaacct cccccacggg gaacgtacag atcgacctcc
    13921 cgattgagcg gatacccggt atcgaaatac cactcgagcg gctcagatcg ctcgttcacg
    13981 accgtgaacc tgatccagta cgcggaattc gtgaacccga aacccaggct ctcatgcgcg
    14041 gaggccttcc acgccacggt tccccgcgcg gtgttctcca ggagcgattg aagcgacagg
    14101 gcgcccgttg tgtcctcgca atactcgacg tgacgcccga tgggagaacc ggaaagcgtt
    14161 cgttcggtaa gccgaattgg ctccacggca tacgacgaac tgtacatcag cgcgatgaac
    14221 accagtccga cgatcgtgcc cgacagggct gctttgacgc ggttcattgt acggttacca
    14281 ttattgcgca tatccttcgc cgtttcatag cggcagtgaa aaccagaagg tgcttccctt
    14341 cccgtattcc gattcgaatc cgacgtctcc gccatgcagt tccacgagtc gcttcgatat
    14401 ggccaaccca agcccggtcc ccttgaaatt gcgcgcgtcc tcggcccgct cgaagtcctc
    14461 gaacacccgc tcccgatcct tttcgcgtat cccgattccg ctgtcgcgaa tggaaaacct
    14521 gacccggtcc ccgtcccgcc gcacctcgaa atcgatgtcg ccgtcgtcgg tgaatttgat
    14581 ggcgttcgaa aacagattca tcagcacctg gccgatttta tatcgatcga gccgcatgat
    14641 atccggaacg tcctcccgta tcacactggt gatgcggaga tgctccttgc ccatcgaccg
    14701 cgcgctgctc cgggcattcg ttacggccga atcgaccacc tcccgggtcc tgctcgtggt
    14761 cttgtggacc gcctcgctcc ccgactcgat catcgcaagg ttgagaaccg agtcgatgat
    14821 ctcgagcagg tgcaggctgg cgtccttgac gcgctgatac gcatcgcggg tctggttctc
    14881 ttcggcatcg atcagggcgg cgatgtcgtc gacgagggcc ttcgcttccg tgctggcggc
    14941 gcaagcggac gaacggatgc caacctcgag cacccccggt acacaccgat ggccattgcc
    15001 ttcgttcgag tataatccga tgagcgagtc aagcaatgcc tgcagggcga tggaatcgat
    15061 cgttccgcat cgggattcct cctcccgcag gagacgcgcc agggatctga gcttgccgag
    15121 cacctcttca tcacaggcgt gcgaaccgaa acggagcatg tcgatgacgc cgttgatcga
    15181 attcagcggg gtgcgcagtt catggctcat gctcgccagg aatcggctct tcgtgcgatt
    15241 cgccacctcg gcggcgtcct tggtccgccg aagctcctca tcggcgcgta cccgttccgt
    15301 gatgtcgcgc gagatgccgg tatactcaca cgtccccgtc ggcgccttct ccaggcgaac
    15361 ccgttgcccg agccaaatca tccggccgtc cccacgcagc accggaaact cgagataggt
    15421 ttcatcgatc ttctcttcgt actggcgctt gaa
//
LOCUS       DAORHF010000067        15415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_2910_length_15415_cov_5.588932, whole genome shotgun sequence.
ACCESSION   DAORHF010000067 DAORHF010000000
VERSION     DAORHF010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15415
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2910_length_15415_cov_5.588932"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /gene="gdhA"
                     /locus_tag="PKJ16_07605"
     CDS             complement(<1..1156)
                     /gene="gdhA"
                     /locus_tag="PKJ16_07605"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HNV46890.1"
                     /translation="MSLINEIIDKVRKQDPEQKEFHQAVQEVMETLEPTINKHPEFVK
                     SKVYERIVEPDRVIMFRVPWLDDKGEVQVNRGFRVQFNNAIGPYKGGLRFHPSVNLGI
                     IKFLGFEQIFKNSLTTLPMGGAKGGSDFDPKGKSDMEVMKFCQSFMRELFRHVGPDID
                     VPAGDIGVGGREIGYMYGYYKKIRNEHTGVLTGKGLEYGGSLIRPEATGYGAVYFAAE
                     MMATKKMDYAGKTVVVSGAGNVAQYTVEKVNELGGKVIALCDSNGTIVDEAGIDNQKL
                     QYVMELKNIKRGRIKEYADKYKAKFFADLSVWDVIREQSLKVDIAFPSATQNEIDGKN
                     AEALVKNGCKCVSEGANMPSTPDAIKFYQEYGILYGPGKAANAGGVSTSGL"
     gene            complement(1382..3964)
                     /locus_tag="PKJ16_07610"
     CDS             complement(1382..3964)
                     /locus_tag="PKJ16_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV46891.1"
                     /translation="MNSIETASTGIESLDRITTGLRMGDNVVWQFDDIDDYRAFAVPF
                     AERAMRENRRVVYLRFAGHEPIIGPHQYSVLYTLDAFTGFEDFSARVFQIITNEGVDV
                     FYVFDCLSDLQSMWATDLMIGNFFRVTCPYLFELNTIAYFAILRNRVSFSTIARIRDT
                     TQVLIDVYHTGGTYYVHPLKVWNRYTPTMFFPHLRASDDLIPLTNSVDLTTLMSHILD
                     GDTRASLRNLDYWDLLFLEAEELMNARPDSPEAREMMAKLCSLVIGRDERMLALASGH
                     FTLRDLVEIKNRLIGTGYIGGKSVGMLVSRKILMNANTASFGALLEPHDSFFIGSDVF
                     YTYIVQNGWWRLFMEHKTEEGYYPAAEKLNALMLGGTFPDAIKEKFQQLIDYFGQSPI
                     IVRSSSLLEDAFGNAFAGKYDSFFLVNQGSPAERYRQLIDAIQRVYASTMNVDALTYR
                     EQRGLNRMDEQMALLVQRVSGSHCSRYFFPDLAGVGLSYNTYVWKNTLDAKAGMMRIV
                     YGLGTRAVNRVEGDYPRIVALDDPLLKAQAGMYDARKFSQHYADVLNVFKNEIETIPV
                     SDVINNTNEMDLSLIGARDTEAEEMIRRQGRDPEAMWILTFDDIFTRTPLASHMRGIL
                     ATVADAYGAPVDIEFTINFLGPREYRINLLQCRPQQTKWHDRVVEMPAAIDDGRVLFR
                     TNGNFLGGSVSMPIRRVIAIDPLRYGELEQTKKYDIARLVGKLNRLIPDTASLPTLLM
                     GPGRWGTTTPALGIPVRFSEINRIAVLVEVAVMRDDFAPELSYGTHFFQDLVETDIFY
                     AAIFPDRDGVRLNSTLLSTRPNLLTALLPDESAYADIVSVYDVDSLRLMADIVSQRLL
                     CFEE"
     gene            4223..5290
                     /locus_tag="PKJ16_07615"
     CDS             4223..5290
                     /locus_tag="PKJ16_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase family protein"
                     /protein_id="HNV46892.1"
                     /translation="MCGIVGIINTDRRLIDGSHIREAIRIQRDRGNGLGGGFAAYGIY
                     PENKDQYAFHLMYEGDRKSPIVAKVEAYLQNRTRVYQSEEIPVYPNDRVAGGPYFKRY
                     FVKPLAEEIRPDQSEEDYIVETVMFINRMGGAFVVSSGKNMGAFKGVGYPEDIADYFH
                     IQDYKAYMWTAHNRFPTNTPGWWGGAHPFTILDKAVVHNGEITSYGTNMRYLEMFGYF
                     CLLQTDTEVISYIFDKLTRKDGLSRDDACFAMAPALWEEIDRIDDRKKKKIRQVYGAA
                     VVNGPFGIVLTHKDGAIILNDRNKLRPVVCARTGNTWYASSEECSIRLLSPEVDELWS
                     PKGGEPVLIELGADTGTREVA"
     gene            5290..6813
                     /locus_tag="PKJ16_07620"
     CDS             5290..6813
                     /locus_tag="PKJ16_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010939110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HNV46893.1"
                     /translation="MGIMQRNWSVIPPFFPEFDMERCDCANGCTICSRECSFGEISQR
                     PVRGADGKVYYRPWANKAACGACQRCAKMCPHGAITIRRAPMHTSHDYWTTDYVNNIW
                     KQSGNEGGVLLVGNGATGPQRRYFDHLMFDACQVTNPSIDPLREPMEIRTFLGRKPDT
                     IDGDMGPQLALEVPIMFGAMSFGALNLRVHEAEARATKAVGTFWNTGEGGFHKSLRGY
                     GANTIVQVASGRFGVSEEYLKSAAAVEIKIGQGAKPGIGGHLPGEKVGPAISETRMIP
                     VGSDALSPAPHHDIYSIEDLRQLIFAIKEATEYQVPVSVKIAAVHNVAPIVSGIARAG
                     ADIIAIDGFRGGTGATPLQIRQNTGIPIELAIAAADQRLREEKIRGSVSLIASGGIQC
                     SSDVLKALCLGADAVYVATPVLIALGCGVCQRCFSGKCAYGITTNKPGLAERIDVDEA
                     TEALTRLLRSWAEEIEELMGSMGISTIESLRGNRDRLRGVGLSEEELTILGVKHAGA"
     gene            6832..9000
                     /locus_tag="PKJ16_07625"
     CDS             6832..9000
                     /locus_tag="PKJ16_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV46894.1"
                     /translation="MMTVDAQGIYYRMLNRRIREQCAMGTPELVITNVMGQRYIGGGI
                     NGSTNIKIYGTPGQDLGAFMNGLTLEVFGNAQDGVANTMNAGKIIVHGKAGEIPGHSM
                     RGGKVFIRDDVEYRAAIHMKEYEGQVPWVIIGGTAKDYCGEYMAGGRVVVLNIANRPG
                     SPVGYSVGTGIHGGAIYVRGAIEPFQLGRGAIVASLDDDDVAFLKGALEEYSRDLSIN
                     LGNITFYEFVKVTKKGHRPFANLYTPAMNIKTTQPKHINLTPPCAYNCPTGIPTSVYL
                     NLIKEGKIREAQALMDEYTPFRMSVCGTVCPAPCMTGCSRNMIDGPVEIQKIAREYYP
                     EFPPEPAKKKRRELVSVIGAGPAGLSAAWQLARRGYRVAVYDSADDVGGKVRKAIPRE
                     RLADELLDRDIERIKTYPIEWKLRTPITGALFSELHAMSDAVVVATGAQVPRRIPYPG
                     GERIISGLTFLTDIIEGRPMDLSGKEVAVIGAGNVGMDIACESWRLGAKKVTAIDIAK
                     PLAFGKELEMARRLGTEIVWPKTIERLDERRVVFSDGTSLKADVVFFSIGELPDAGFL
                     GDAVLRDERGYLVTAEHSFRSNDPKVYGCGDIIRPGLVTDAIGTGRLAAMEVHANLTG
                     GTFVYPAKSLVPRRRIQTVYFGGETREIDRCISCGTCIFCDRCIDACPQDALSRNGEV
                     FSVDPARCTGCYTCVNVCPRGVLQTDDVAEFVLDELDNTG"
     gene            complement(8997..11174)
                     /locus_tag="PKJ16_07630"
     CDS             complement(8997..11174)
                     /locus_tag="PKJ16_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV46895.1"
                     /translation="MKLAFALIIFVLATASVPAAVAHDDVAMTLGAEVVSVPVGPYAY
                     CLEDPSGALSFERVTAPPAAKSFIRNERSVPAWGFTRSAFWLRFSFVADANAPRDWVL
                     ELSYPLMDFIELYVPDERGSYRRIDAGYRYPFSQRGNGHPLFVFSLSVKPGARGTAYL
                     RLAGGDRIEAPLTLWRADAFHDKTINEQYVLGMYYGLMLVIVIFNLIMFISVRDRAYL
                     RYVFFIIAIGFYQLTQDGLAYKFLPAGITPFNHYIIASIAAIEVFAILFTQSYLNTAT
                     MVPRLHRALFVVLMVPVCIFAASFVLDYTTSIQLIVITTLISIGLALFVGVRVLLANY
                     RPARFFMAAWGFTLTGAAISSLKNLGVLPANFFTIYSLHIGTAIELIILTIGLGDRIT
                     ILRREKDAAQAEKLDAQRIMVESLMQSHRELEDAFRELARTEERYRRLVEDSSDIIFT
                     LDNAGTVLSINRAIERHFSVSPDMVIGMHFQTLLFRGGGPTDSNRFVIEKLEQFLRDR
                     ETAQFKAEFRSPIVSEPKEMLVQLESLDFSGREEIRGTASPITEDTLLSALVEDRQTY
                     RIGNYLLAAEDVSHRITRNLRRYEDQHTTTMLRVALREMIINAIEHGNLGISYDEKTR
                     AITEGTYFEFVSTRQADPRFRDRTVTIEYHITSDGCRYTVTDEGDGFDHRARMSVSVA
                     ELNSRREIHGRGILMATSFFDAISYNETGNAVTLVKRFRGGRP"
     gene            complement(11287..11769)
                     /locus_tag="PKJ16_07635"
     CDS             complement(11287..11769)
                     /locus_tag="PKJ16_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46896.1"
                     /translation="MKLKHFASIALVAVFMVSLSACKKQEEKAKPEKAPTVALTELVK
                     YLPKFDKAFKDLEFDQYSTTGTWLQINVLKKGKKDRVMWFQVFDQRNEQSTKDGYASA
                     TEKVGKYPAKVAKDSFVWILANNIEIRLVADDKNKEFKNTDKLKKFLEAFDLAGLEKL
                     "
     gene            complement(11865..12863)
                     /locus_tag="PKJ16_07640"
     CDS             complement(11865..12863)
                     /locus_tag="PKJ16_07640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNV46897.1"
                     /translation="MEMPLLVVLAVVPPILFLLYILRYDRLEPEPLSLIARALFLGAI
                     AVIPAGTIELLLQPLPLFKSGGLVGAGLTSFILIAPVEEAAKLAVVLAFFWKNQNFNE
                     ENDGIVYTGSVAVGFALAENVLYVIESGLAIGILRAITSIPLHTFTGVLMGYFVGMAK
                     FAETPAASKRLILRGFLIAYVVHAAYDTVVLSGTAAALLMVPIVVVLFIAGFRFLKRG
                     QALSAKRWEHGPSPASATPSPDAPSPDMPRETHPGPSVAPQEIGVYRIVISRTLFALT
                     AVFWALMALGIVTSGPEDEIEMLDAIAGGIILTFVPVVAAITLELSHARLRKASAL"
     gene            complement(12919..13389)
                     /locus_tag="PKJ16_07645"
     CDS             complement(12919..13389)
                     /locus_tag="PKJ16_07645"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV46898.1"
                     /translation="MIDRLKKFAPHSIGPLIIAIGIAVHAHESGVRDRFATAPGVVTH
                     AEIYTQGSGPRTTVHVLVKYSYTVGGAAYESHGIGMARIFYKTREEAERAIAPYPTGG
                     AVTVHYDTADPRTAYLDVAGPGNGQLAIVLGVLVGLVISPVTMLIRRRSRRETA"
     gene            complement(13386..>15415)
                     /locus_tag="PKJ16_07650"
     CDS             complement(13386..>15415)
                     /locus_tag="PKJ16_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV46899.1"
                     /translation="QPERTAAREQHGMHPVDAAEAGTMQVALAVVAVTATIIAVFLPM
                     GFMQSMVGQFFKQFGLTVVFALLISLFDAMTMAPLLSAYFAGKQHGRMNWVVRKFDEF
                     HTWLEHTYGRLIELCLDRPKTVIAIMSIVFLASMVSVKFIKKTFQPQSEQGEFMITME
                     LTPGISLDGTERFAMKVFEKVKNLPEVDRISIVVGGDQGEPYKSTLGVNLLPVSKRKR
                     GSIEVQRVVREMFATDEFKGAKIMVQHYSTTGGSNYPFTMNIRGDNLEELETYATTLI
                     DIMNRKVKDLIEIKSSLEGGKPEFQVVLDPRMMDLVGVTSGMAGQELRYHVAGEKVGK
                     LHQSGLEYNIRLRLKPEQRDLEKFYYQTKVPNSGQQQKLIPVSAISEPVKKLGPSFIM
                     RENRQRVVQINANTAPDGGVGDAIDAVKGIFKKELPLKAGMTYGFIGAAEAFGEMQTD
                     MLMVFVLALVFIYLVLASLYESFITPVTILAAIPPAVSGAFLALLVTREMLSMMAWIG
                     LIMLIGLVTKNSILLVDFIVTGEREGKSRRDAIREAGMVRLRPILMTTFAMIAGTMPV
                     ALGWGEAAGMRTSMGIVIVGGLLVSTLITLFVVPSMYGYVDSAREWIESKFRPRYEMQ
                     DHLAHVGGQQATETTEEIVAHSETTEVEMTISVPRQAPSRSKAKTGGRKKR"
BASE COUNT         2899 a         4635 c         5075 g         2806 t
ORIGIN      
        1 ccaggccgga ggtggatacg ccgccggcat tggcggcctt tccgggaccg tagaggatgc
       61 cgtattcctg atagaacttg atcgcatcgg gcgtggacgg catgttcgcg ccctccgaca
      121 cgcacttgca accgttcttg acaagcgcct cggcgttctt gccgtcgatc tcgttctgcg
      181 tcgccgacgg gaaggcgatg tcgaccttga gggactgctc gcggatcacg tcccacacgc
      241 tcaggtcggc gaaaaatttc gccttgtact tgtcggcgta ttccttgatg cgcccacgct
      301 tgatgttctt gagctccatc acgtactgga gtttttgatt gtcgatgccg gcctcgtcaa
      361 cgatggtgcc gttggagtcg cacagcgcga tcaccttgcc gccgagctcg ttcaccttct
      421 cgaccgtgta ctgggccacg ttgcccgcgc cgctgacgac gacggtcttg cccgcatagt
      481 ccattttctt cgtggccatc atctcggcgg cgaaatacac cgcgccgtag ccggtcgcct
      541 cgggacggat caggctcccg ccgtactcga gacccttgcc cgtgaggacg cccgtgtgct
      601 cattgcggat cttcttgtag tagccgtaca tgtagccgat ctcccggccg cccacgccga
      661 tgtcgccggc gggtacgtcg atgtcgggac cgacgtgtcg gaagagttcg cgcatgaagg
      721 actggcagaa tttcatgacc tccatgtccg atttgccctt ggggtcgaag tccgatccgc
      781 ccttggcgcc gcccatgggg agggtggtca gcgagttctt gaagatctgc tcgaagccca
      841 gaaatttgat gatgccaaga ttgaccgacg ggtggaagcg gaggccgccc ttgtagggcc
      901 cgatcgcgtt gttgaactgg acgcggaagc cccggttcac ctgcacctcg cccttgtcgt
      961 ccagccacgg cacgcgaaac atgatgacgc ggtccggctc cacgatgcgc tcgtacacct
     1021 tggacttcac gaactcgggg tgcttgttga tcgtcggctc aagcgtctcc atgacctcct
     1081 ggaccgcctg atggaactcc ttctgctcgg ggtcctgctt tcgcaccttg tcgatgatct
     1141 cgttgataag tgacataatg caacgtctcc tttactgtga ttttatccgt gagaaccact
     1201 atcgcatgcc gcatgaagac ccgcttcatg atagcaatac tgatagattt acaataattt
     1261 ttagctgcag taaggttttc gtccgtgatg gacctgtcaa ttagtatcac gttaaacgcc
     1321 ccgttgtaat gttacatcca tgtaaatttt tatcggaacg tggggtatga tgattaggcc
     1381 ctcattcctc gaagcacagg aggcgttgcg atacgatgtc ggccatgagg cggagcgaat
     1441 cgacatcata tacgctgacg atgtcggcgt aggccgattc gtccggcagg agcgcggtga
     1501 gcaggttcgg ccgggtcgag aggagcgtcg aattgagcct cacgccgtcc cggtcgggga
     1561 agatggcggc atagaagatg tcggtctcga ccaggtcctg gaagaaatgc gtaccgtacg
     1621 agagctcggg cgcgaaatcg tcgcgcatca ccgccacctc caccaggacc gcgatgcggt
     1681 tgatctccga gaagcggacg gggatgccca gggcgggcgt cgtggtcccc cagcgccccg
     1741 ggcccatgag cagggtggga agggacgccg tgtcggggat gaggcggttg agcttgccca
     1801 ccaggcgcgc gatgtcgtat tttttcgtct gttccagctc gccgtagcgc aagggatcga
     1861 tggcaatgac ccggcgtatg ggcatcgata cgctgccgcc gaggaagttg ccgttcgtgc
     1921 gaaacaggac ccgcccgtcg tcaatggcgg cgggcatctc cacgacgcga tcgtgccact
     1981 tggtctgctg cggccggcac tgcagcaggt tgatccggta ttcgcggggg ccgaggaagt
     2041 tgatcgtgaa ctcgatgtcg accggcgcgc cataggcgtc cgcgaccgtg gcaaggatcc
     2101 cgcgcatgtg cgaggccagc ggcgtccggg tgaagatgtc gtcgaaggtc aggatccaca
     2161 tcgcctcggg gtcgcgcccc tggcggcgga tcatctcctc ggcctcggtg tcccgcgcgc
     2221 cgatgagcga caggtccatc tcgttggtgt tgttgatgac gtccgagacg gggatcgtct
     2281 cgatctcgtt cttgaagaca ttgaggacgt cggcgtagtg ctgcgagaac ttgcgcgcat
     2341 cgtacatgcc cgcctgggcc ttgagcagcg ggtcgtccag cgctacgatg cgcggatagt
     2401 cgccctcgac gcggttcacc gcgcgggtgc cgagcccgta gacgatgcgc atcatccccg
     2461 ccttcgcgtc cagggtgttc ttccatacat aggtgttgta cgaaagcccc acgcccgcca
     2521 ggtcggggaa gaagtaacgc gagcagtgcg aacccgacac gcgctggacc aggagggcca
     2581 tctgctcgtc catgcggttg aggcctcgct gctctcggta ggtgagggcg tcgacgttca
     2641 tcgtgctcgc gtacacgcgt tggatcgcgt cgatgagctg ccgatagcgc tcggcgggcg
     2701 acccctggtt caccagaaag aagctgtcgt actttccggc gaaggcgttg ccgaaggcgt
     2761 cctccaggag gctgctggag cgcacgatga tgggggactg cccgaagtaa tcgatgagct
     2821 gctggaactt ttctttgatc gcatcgggaa aggtgccgcc cagcatgagc gcgttgagct
     2881 tctccgcggc cgggtagtag ccctcttcgg ttttatgctc catgaacagg cgccaccagc
     2941 cgttctgcac gatgtaggtg tagaagacgt cggagccgat aaaaaaggaa tcgtgcggct
     3001 cgagcaatgc gccgaaggaa gcggtgttgg cgttcatgag gatcttccgt gagacgagca
     3061 tgcccaccga ttttccgccg atatagccgg tgccgatgag ccggttcttg atctccacca
     3121 ggtcgcgcaa cgtgaaatgg ccggaggcga gcgcgagcat ccgctcgtcg cgcccgatga
     3181 caagcgagca gagcttcgcc atcatctcgc gcgcctccgg cgagtccgga cgcgcgttca
     3241 tgagctcctc cgcctcgagg aacagcaggt cccagtagtc cagattgcgc agcgacgcgc
     3301 gcgtgtcgcc gtccaggatg tgcgacatga gcgtggtgag gtccacgctg ttggtgagcg
     3361 ggatgaggtc gtcgcttgcg cgcaggtggg ggaagaacat cgtgggcgtg taacggttcc
     3421 acaccttgag cgggtgcacg tagtacgtgc cgccggtgtg gtatacgtcg atgagcacct
     3481 gcgtggtgtc gcggatgcgc gcgatggtcg aaaacgagac gcggttgcgc aggatcgcaa
     3541 agtacgcgat ggtgttgagc tcgaagaggt aggggcaggt gacgcgaaaa aagttgccga
     3601 tcatcaggtc ggttgcccac atcgattgca ggtccgaaag gcagtcgaaa acgtagaaga
     3661 cgtctacgcc ttcgtttgtg atgatctgga agacgcgcgc ggagaagtcc tcgaatccgg
     3721 tgaaagcgtc cagggtatac aggacgctgt actggtgggg cccgatgatg ggttcgtgcc
     3781 ccgcgaaccg caggtagacc acccgccggt tttcccgcat ggcgcgctcc gcgaagggga
     3841 ccgcgaaggc gcggtaatcg tcgatgtcgt cgaactgcca cacgacattg tcgcccatgc
     3901 gcaggccggt ggtgattctg tccaggctct cgatcccggt gctggcggtt tcgatggaat
     3961 tcatggtgcg ccccctcggt gtgatttcgc tcgccgcccg ccgggggcgg gatcggcacg
     4021 ataaacgtag cacatgctgt aagtggcgat aaaattaata catatcatta ctgactaccg
     4081 cgcccggttc cggcaaaagt ttacatgcgc gaaacattgc agcggaacat ttaaattgaa
     4141 accagggggg catggcggtt tattaaccat cggttacacg gacggcagtg actttagcga
     4201 tgctaacaac aaggagaccg gcatgtgcgg catagtggga atcatcaaca ccgaccggcg
     4261 cctcattgac gggagccaca tccgcgaggc gatccgcatc cagcgggacc gcggcaatgg
     4321 cctcggcgga gggttcgcgg cgtacggcat ctatccggag aacaaagacc agtacgcctt
     4381 tcatctcatg tacgaggggg atcgcaagag ccccatcgtc gcgaaggtgg aggcctatct
     4441 gcaaaaccgc acgcgggtgt accagtccga ggagatcccg gtgtatccga acgaccgggt
     4501 ggcgggcggt ccgtacttca agcgctattt cgtgaagccc ctcgcggagg agatccggcc
     4561 ggaccagagc gaggaggatt atatagtgga aaccgtcatg ttcatcaacc ggatgggcgg
     4621 cgccttcgtc gtctcctccg gaaagaacat gggcgccttc aagggcgtgg gctatcccga
     4681 ggatatcgcc gattacttcc acatccagga ctacaaggcc tacatgtgga cggcgcacaa
     4741 tcgcttcccc acgaacacgc ccggctggtg gggcggcgcg cacccgttca ccatcctgga
     4801 caaggcggtg gtgcataacg gcgaaatcac cagctacggg accaacatgc gctacctcga
     4861 gatgttcggc tacttctgcc tgctgcagac ggacacggag gttatcagct atatcttcga
     4921 caagctcacg cgcaaggacg gcctctcccg ggacgacgcc tgcttcgcca tggcgccggc
     4981 gctctgggag gagatcgacc gcattgacga ccggaagaag aaaaagatcc ggcaggtgta
     5041 cggcgccgcg gtggtgaacg gccccttcgg gatcgtgctc acgcataagg acggcgccat
     5101 catcctgaac gaccgcaaca agctgcgccc ggtggtgtgc gcccgaacgg ggaacacctg
     5161 gtacgcctcg tcggaggaat gcagcatccg gctgctctcc cccgaggtcg atgagctctg
     5221 gtcccccaag ggcggcgagc cggtgctgat cgaactgggc gccgataccg ggacccggga
     5281 ggtggcgtga tgggcattat gcaaagaaac tggtccgtga ttcccccgtt tttccccgag
     5341 ttcgacatgg agcggtgcga ttgcgccaac ggttgcacca tctgctcccg cgagtgttcc
     5401 ttcggcgaga tttcgcaacg tcccgtgcgc ggcgcggacg ggaaggtcta ctatcgcccc
     5461 tgggcgaaca aggccgcctg cggcgcctgc cagcgctgcg cgaagatgtg cccgcacggc
     5521 gcgatcacca tccgtcgcgc gccgatgcac acgagccacg attactggac cacggactac
     5581 gtgaacaata tctggaagca gtcgggcaac gagggcggcg tccttctcgt gggaaacggc
     5641 gccaccgggc cgcagcgtcg ctacttcgac catctgatgt tcgacgcctg ccaggtgacc
     5701 aatccctcca tcgacccgct gcgcgagccg atggagatcc gcaccttcct gggacgcaag
     5761 cccgacacca tcgacggcga catgggaccg cagctcgcgc tcgaggtgcc catcatgttc
     5821 ggtgcgatga gctttggcgc gctcaacctg cgcgtgcacg aggccgaggc gcgcgcgacg
     5881 aaggccgtgg ggaccttctg gaacaccggc gagggcggtt tccacaagtc gctgcgcgga
     5941 tacggcgcga acaccatcgt gcaggtcgcc tcgggccgct tcggcgtgag cgaggaatac
     6001 ctgaagtcgg ccgcggcggt ggagatcaag atcggtcagg gcgcgaagcc ggggatcggc
     6061 gggcacctgc ccggcgagaa ggtgggaccg gccatctccg aaacccggat gatcccggtg
     6121 ggaagcgacg cgctctcccc ggcgccgcac cacgacatct actccatcga ggacctgcgg
     6181 cagctcatct tcgcgataaa agaagcgacc gaataccagg tgccggtctc ggtgaagatc
     6241 gcggccgtgc acaacgtcgc gcccatcgtc agcggcatcg cgcgcgcggg cgccgacatc
     6301 atcgcgatcg acggcttccg gggcggcacg ggcgccacgc cgctgcagat ccggcagaac
     6361 accggcatac cgatcgagct cgcgatcgcc gccgccgacc agcgactgcg cgaggagaag
     6421 atccgcggtt ccgtgagcct gatcgcgtcg ggcggcatcc agtgctcctc cgacgtcctc
     6481 aaggccctct gcctgggcgc cgacgccgtc tacgtcgcga cgccggtgct catcgcgctg
     6541 ggctgcggcg tatgccagcg gtgcttctcc ggtaagtgcg cctacgggat caccaccaat
     6601 aaaccggggc tggccgagcg gatcgacgtc gatgaggcga ccgaggcgct cacgaggctc
     6661 ctgcggtcgt gggcggagga gatcgaggag ctgatgggct cgatgggcat cagcaccatc
     6721 gaatcgctcc ggggaaaccg cgaccgcctc cgcggcgtgg gcctgagcga agaggaattg
     6781 accattctcg gcgtaaagca tgccggggcg taaggagcgg gaggggcgac aatgatgacc
     6841 gtggatgcac agggaatcta ctaccggatg ctgaaccgcc gcatccgcga gcagtgcgcg
     6901 atggggacgc cggagctcgt catcaccaat gtgatgggac agcgctatat cggcggcggg
     6961 atcaacggat cgacgaacat caagatatac gggaccccgg gccaggacct gggggccttc
     7021 atgaacgggc tcaccctcga ggtgttcgga aacgcgcagg acggcgtggc caacacgatg
     7081 aacgcgggca agatcatcgt gcacgggaag gccggggaga tccccgggca ctcgatgcgc
     7141 ggcgggaagg tcttcatccg cgacgacgtg gagtaccggg cggccatcca catgaaggaa
     7201 tacgagggcc aggtgccgtg ggtcatcatc ggcggcaccg cgaaggacta ctgcggggag
     7261 tacatggccg gcggtcgcgt ggtggtgctc aacatcgcaa accggcccgg ctcacccgtg
     7321 ggctacagcg tggggaccgg cattcacggc ggggcgatct atgtccgcgg cgcaatcgag
     7381 cccttccagc tcggacgggg cgcgatcgtc gcatcgctcg acgacgacga tgtcgctttc
     7441 ctgaagggcg cgctcgagga gtactcccgc gatctttcga tcaacctggg aaacatcacc
     7501 ttctacgagt tcgtcaaggt caccaagaag gggcaccggc cgttcgccaa tctctacacc
     7561 ccggcgatga acatcaagac cacgcagccc aagcacatca acctcacgcc gccgtgcgcc
     7621 tacaactgcc caaccggcat accgaccagc gtctacctca acctcatcaa ggaggggaag
     7681 atccgcgagg cgcaggcgct catggacgag tacacgccct tccgcatgag cgtgtgcggg
     7741 accgtatgcc cggcgccgtg catgacggga tgcagccgga acatgatcga cggcccggtg
     7801 gagatccaga agatcgcgcg cgagtactat ccggagttcc ctccggaacc ggcgaagaag
     7861 aagcggcgcg agctcgtttc cgtcatcggc gcgggtcctg cggggctctc ggcggcctgg
     7921 cagctcgccc ggcgcggcta ccgggtcgcg gtgtacgatt ccgccgacga tgtgggcggc
     7981 aaggtgcgca aggccatccc gcgcgagcgc ctcgcggacg aactgctcga ccgcgacatc
     8041 gagcggatca aaacctaccc gatcgagtgg aagctcagga cgcccatcac cggcgcgctc
     8101 tttagcgagc tgcatgcgat gagcgatgca gtggtggtcg cgaccggcgc gcaggtgccg
     8161 cggcggatcc cctatcccgg cggggagcgc atcatctccg ggcttacgtt cctcacggac
     8221 atcatcgagg ggcggccgat ggacctctcc ggcaaggagg tcgcggtgat cggcgcgggg
     8281 aacgtgggca tggacatcgc ctgcgaatcg tggcggctgg gcgcgaagaa ggtcacggcc
     8341 atcgacatcg ccaagcccct ggccttcgga aaggaattgg agatggcgcg ccgtctcggg
     8401 accgagatcg tgtggccgaa gaccatcgag cgcctcgacg agcggagggt ggttttttcc
     8461 gacgggacgt ccctcaaggc ggacgtggtc tttttcagca tcggcgagct tccggacgcg
     8521 gggttcctcg gcgacgcggt gctgcgcgac gagcgcgggt acctcgtgac ggccgagcat
     8581 tcgttccgct ccaacgaccc aaaggtgtac ggctgcggcg atatcatccg tccggggctg
     8641 gtcaccgacg ccatcggtac gggccgtctc gcggcgatgg aggtccacgc gaacctcacg
     8701 ggcgggacct tcgtgtatcc ggcaaagagc ctggtgccgc gccgacgcat ccagacggtc
     8761 tacttcggcg gggagacgcg cgagatcgac cggtgcatct cctgcgggac ctgcatcttc
     8821 tgcgacaggt gcatcgacgc ctgcccgcag gacgcgctgt cgcgcaacgg cgaggtcttc
     8881 agcgtcgatc ccgcgcgctg cacgggatgc tacacctgcg tgaacgtgtg cccgcgcggc
     8941 gttctccaaa ccgacgacgt ggccgagttc gtgctggacg agctggacaa tacgggctag
     9001 gggcgaccgc cccgaaagcg cttcacgagc gtgaccgcgt ttcccgtttc gttgtaggag
     9061 atcgcgtcga aaaagcttgt ggccatgagg atcccccggc cgtgtatctc gcggcggctg
     9121 ttgagctccg cgaccgagac gctcatgcgc gcgcggtggt cgaacccgtc gccctcgtcg
     9181 gtgacggtgt accggcagcc gtcggaagtt atgtgatact ctatggttac cgtccggtcg
     9241 cggaagcggg ggtcggcctg ccgggtcgag acgaactcga aataggtccc ctcggtgatg
     9301 gcgcgggtct tttcgtcgta tgagatgccc agattgccgt gctcgatcgc gttgatgatc
     9361 atttcgcgca gcgccacgcg cagcatggtg gtcgtatgct ggtcctcata gcggcgcagg
     9421 ttgcgggtga tgcgatgcga gacgtcctcg gcggccagca ggtagtttcc gatgcgataa
     9481 gtctgccggt cctccaccag cgccgagagc agcgtgtcct cggtgatggg cgaggcggtc
     9541 ccccggatct cttcgcgccc ggagaagtcg aggctctcga gctgcaccag catctctttg
     9601 ggttcgctca cgatgggcga gcggaactcg gccttgaact gcgccgtctc ccggtcccgc
     9661 aggaactgct cgagcttttc gatcacaaag cggttcgagt ccgtcggacc gccgccccgg
     9721 aagagcaacg tctggaagtg catgccaata accatgtcgg gcgatacgct gaaatgccgt
     9781 tcgatggcgc ggttgatgga gagaacggtg cccgcattgt ccagcgtgaa gatgatgtcg
     9841 ctggagtcct caacgagacg ccggtagcgt tcctccgttc gcgcgagttc gcggaaggcg
     9901 tcctcgagct cgcggtgcga ttgcatcaag ctctccacca tgatccgctg ggcgtccagt
     9961 ttctccgcct gcgcagcatc cttttcgcgg cgcagaatgg tgatccgatc gcccaggccg
    10021 atggtcagga tgatgagctc gatggcggtg ccgatatgga gcgagtagat ggtgaaaaag
    10081 tttgccggga ggacgcccag gtttttcagg gacgaaatgg cggccccggt gagcgtgaag
    10141 ccccaggccg ccatgaagaa acgcgccggc cgatagttgg ccagcagcac cctgacgccc
    10201 acgaacaggg ccaatccgat gctgatcagg gtggtgatga cgatcaactg gatggaggtc
    10261 gtgtagtcga gaacgaacga tgcggcgaaa atgcataccg gcaccatgag gaccacgaag
    10321 agggcgcggt ggaggcgcgg caccatggtg gcggtgttca ggtagctctg cgtgaaaaga
    10381 atggcgaaca cctcgatcgc cgcaatgctc gcgatgatgt agtgattgaa cggcgtgatc
    10441 cctgcgggga ggaatttgta ggccaggccg tcctgcgtga gctggtaaaa cccaatcgca
    10501 ataataaaga aaacatatcg cagataggcc cggtcccgca ccgaaatgaa catgatgaga
    10561 ttgaaaatga cgatgacgag catgagcccg tagtacatgc ccagcacgta ttgttcgttg
    10621 atcgttttat cgtggaaggc gtcggcccgc cacagggtga ggggcgcctc gatgcggtct
    10681 cccccggcaa ggcgcagata cgcggttccg cgcgcgccgg gcttgacgga cagggaaaag
    10741 acgaacaggg gatgtccgtt tccacgctgt gaaaagggat atcggtaccc ggcgtcaatc
    10801 cggcggtatg atccccgttc gtccggcacg tacagctcga tgaaatccat aagcggatag
    10861 gaaagctcga gcacccagtc gcgcggcgcg ttcgcatcgg cgacaaagga aaaccgaagc
    10921 cagaaggccg agcgggtgaa gccccaggcg gggaccgacc gttcgttccg aatgaaggat
    10981 ttcgccgcgg ggggcgcggt cacccgctcg aaggacaggg cgccgctcgg gtcctcaagg
    11041 cagtaggcat acggccccac cgggaccgaa acgacctcgg cgccgagcgt cattgcgacg
    11101 tcatcatgcg cgacggccgc cggaaccgac gccgtcgcga gtacgaagat gatgagcgcg
    11161 aatgccagtt tcatagaatg tcgtgcgata ccgccgggcg atacgactca catgacagta
    11221 tcgacggagg cgtgggagag aaatgcgcga ccccgcgcac ggcgcgcggg gtcggccgca
    11281 cagcgattac agcttttcca gcccggcgag gtcgaaggcc tcgaggaact ttttcagctt
    11341 gtcggtgttc ttgaattcct tgttcttgtc gtcggccaca aggcggatct cgatgttgtt
    11401 cgccaggatc cacacgaagc tgtccttggc gacctttgcg gggtacttgc ccaccttctc
    11461 cgtggcgctg gcgtagccgt ctttcgtcga ctgctcattg cgctgatcga aaacctggaa
    11521 ccacatcacg cggtctttct tgcccttctt gagaacgttg atctgcagcc aggtgccggt
    11581 ggtcgaatac tggtcgaact cgaggtcctt gaaggccttg tcgaacttgg ggagatactt
    11641 gaccagctcg gtgagcgcaa ccgtgggcgc cttctcgggc ttggccttct cttcctgctt
    11701 tttgcaggcg gacagcgaga ccatgaatac ggcgacgagt gcgatggatg cgaaatgctt
    11761 caacttcatg tgtaatcctc ctcgacggtg tgttgatatg tactcgccca gagtattcgc
    11821 tccggacgat atgcacaagt aaaaaaacgg ccctggtccc cgtgttagag tgcggatgcc
    11881 ttgcgaagcc gcgcatgcga cagctcgagc gtaattgccg caacgaccgg cacgaaggtc
    11941 agtatgatcc ctccggcgat cgcgtcaagc atctcgatct cgtcctcggg acccgaggtg
    12001 acgatgccga gggccatgag ggcccaaaac accgccgtga gcgcgaagag ggtcctcgag
    12061 atgacgatac ggtatacgcc gatctcctgc ggggctacgg atggccccgg atgtgtctcg
    12121 cgcggcatat cgggagacgg tgcgtccggg gacggggttg ccgaagccgg agacgggcca
    12181 tgttcccagc gcttcgcgga gagcgcctgg ccgcgcttca gaaagcggaa gccggcgata
    12241 aagaggacga ccacgatggg caccatgagg agcgccgccg cggtgccgga caggaccacc
    12301 gtgtcgtagg cggcgtgcac gacataggcg atgaggaatc ctcgaaggat gagccgcttc
    12361 gacgctgcgg gcgtttccgc gaacttggcc atgcccacga aataccccat gagcacgccg
    12421 gtgaaggtgt gcagcgggat cgacgtgatg gcgcgcagga tcccgatcgc caggccggac
    12481 tcgatgacat agagaacgtt ctcggccaat gcgaagccga cggcgacgct ccccgtgtat
    12541 acgatgccgt cgttttcttc attgaagttt tggtttttcc agaagaaggc gagcaccacg
    12601 gcgagcttgg ccgcctcctc gacgggcgcg atgagaatga aggacgtgag tcccgcgccg
    12661 acgagaccgc ccgacttgaa cagcggaagc ggctggagca ggagctcgat cgttccggcg
    12721 gggatcaccg caatggcgcc caggaacagc gcgcgggcga tgagcgagag cggctccggc
    12781 tcgagccggt cgtagcggag aatgtagagc aggaacagga tcggcggaac gacggccagt
    12841 acgaccaaga gcggcatttc catggggcac ctcgcatcga ggctccatga tggcgcggga
    12901 aaaacaagac gtcaagaact atgcggtctc cctgcgcgag cgtcgtcgta tgagcatcgt
    12961 caccggagag atcacgaggc cgacgaggac gcccagcacg atggcgagct gcccgttccc
    13021 ggggccggcg acgtccagat aggccgttcg ggggtcggcg gtgtcgtaat ggaccgtgac
    13081 ggcgccgccc gtgggatagg gggcgatggc gcgctcggcc tcctcgcgcg tcttgtagaa
    13141 gatgcgcgcc atgccgatcc catgcgattc gtaggccgcg ccgccgacgg tgtaggagta
    13201 cttcacgagc acgtgcaccg tggtgcgcgg tccggagccc tgcgtgtaga tctcggcgtg
    13261 ggtcaccacg ccgggggcgg tcgcgaaccg atcgcgcacg ccggactcgt gcgcatgcac
    13321 ggcgataccg atggcgatga tgagcgggcc gatgctgtgc ggcgcgaatt ttttcaggcg
    13381 gtcgatcatc gcttttttct tccgcccgtt ttcgctttcg atcgggacgg cgcctgtcgc
    13441 gggaccgaga tcgtcatctc gacttccgtc gtttccgaat gcgcgacgat ctcctccgtg
    13501 gtctcggtcg cctgctggcc gcccacgtgc gccagatggt cctgcatctc gtagcggggc
    13561 cggaatttgc tctcgatcca ttcgcgtgcg gaatcgacat agccgtacat ggacggcacc
    13621 acgaacaggg tgatgagcgt ggagacgagg agcccgccga cgatcacgat acccatggag
    13681 gtgcgcatgc ccgccgcctc gccccagccc agcgccaccg gcatggttcc cgcgatcatc
    13741 gcgaaggtgg tcatgaggat cggacgcagc cgcaccatcc ccgcctcgcg gatcgcgtcg
    13801 cggcgcgatt tgccttcgcg ttcgccggtc acgatgaagt ccaccaacag gatggagttt
    13861 ttcgtgacca ggccgatgag catgatgagg ccgatccacg ccatcatact gagcatttcc
    13921 cgggtgacca ggagcgccaa aaacgccccc gacacggccg gcgggatggc tgcgaggatg
    13981 gtgaccggcg tgatgaacga ttcgtagagg cttgcgagca ccaggtagat gaacaccagc
    14041 gcgaggacga agaccatgag catgtcggtc tgcatttcgc cgaaggcctc cgcggcgccg
    14101 atgaagccgt aggtcatgcc ggccttgagg ggcagctctt ttttaaagat gcccttgacc
    14161 gcgtcgatgg cgtcgcccac gccgccgtcg ggcgcggtgt tcgcgttgat ttgcacaacg
    14221 cgctggcggt tctcccgcat gatgaaggag ggtcccagct tcttgaccgg ctcgctgatg
    14281 gccgagacgg gaatgagctt ctgctgctgg ccgctgttgg gcaccttggt ctgatagtag
    14341 aatttctcga ggtcgcgctg ctcgggcttg aggcgcaggc ggatattgta ctcgaggccg
    14401 ctctggtgga gcttgcccac cttctccccg gcgacgtgat agcgcagctc ctgcccggcc
    14461 atgcccgagg tcacgcccac caggtccatc atcctcgggt ccaggaccac ctggaactcg
    14521 ggcttgccgc cctccaggct cgacttgatc tcgatgaggt ccttcacctt ccggttcatg
    14581 atgtcgatga gcgttgtcgc gtacgtctcc agctcctcca gattgtcccc gcggatgttc
    14641 atggtgaagg ggtagttgct gccgccggtg gtggagtagt gctgcaccat gattttagcg
    14701 cccttgaact cgtcggtggc gaacatctcg cgcaccacgc gctgcacctc gatcgatccg
    14761 cgcttgcgct tcgagaccgg cagcaggttc acgccgagcg tcgacttgta cggctcgccc
    14821 tggtcgccgc cgacgacgat ggagatgcgg tccacctccg ggagattctt caccttttcg
    14881 aacaccttca ttgcgaatcg ctcggtgccg tcgaggctga tgccgggcgt cagctccatg
    14941 gtgatcatga actcgccctg ctcggactgc ggctggaagg tcttcttgat gaacttgacc
    15001 gagaccatgc tcgcgagaaa cacgatcgac atgatggcga tcaccgtctt gggacggtcc
    15061 aggcacagct cgatgagccg tccgtaggtg tgctccagcc aggtgtggaa ctcgtcgaat
    15121 ttccgcacca cccagttcat ccggccgtgc tgcttcccgg cgaaataggc cgagaggagc
    15181 ggcgccatcg tcatcgcgtc gaagaggctg atcaggagcg cgaagaccac cgtgaggccg
    15241 aactgcttga agaactggcc caccatgctc tgcatgaagc ccatgggaag aaacacggcg
    15301 atgatggtgg ccgtgaccgc gaccaccgcg agcgccacct gcatggtgcc cgcctccgcc
    15361 gcgtcgaccg ggtgcatgcc gtgctgctca cgggcggcag tgcgttcggg ctgga
//
LOCUS       DAORHF010000068        15080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3012_length_15080_cov_4.486057, whole genome shotgun sequence.
ACCESSION   DAORHF010000068 DAORHF010000000
VERSION     DAORHF010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15080
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3012_length_15080_cov_4.486057"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..909
                     /locus_tag="PKJ16_07655"
     CDS             49..909
                     /locus_tag="PKJ16_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46900.1"
                     /translation="MDNIRYHAVNTVAVIIFAYITAITINQVIRFVFAPTFSIPQKAM
                     IEKLAQPRLKPFEEYKSIVDSGFFKIPSETPDVPGVGQGGAGAESVNELLLLGTITGP
                     SHIARALIKKKTEKDPQIFRLYGTVHGHKLIRIDNSKVYLKTGNSVQILDMFAKEGAA
                     ATPAKAPSGTGAALPTSGKVVQNLSKAELQQKVLNNVDNALQGIRAGPYRVDGAIKGY
                     KIFRIKPHNIMYKLGARNGDVLMRVNGHAIDSTEKLLSIWKSVQGDSKISVDLERGGK
                     LLTFDFNITE"
     gene            925..2883
                     /locus_tag="PKJ16_07660"
     CDS             925..2883
                     /locus_tag="PKJ16_07660"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HNV46901.1"
                     /translation="MMISTTGVARIAGILLIGTVIFSVGDVRTQEAPVKEPTREESKQ
                     PVRTEAPAATDKKTTPTIKNDATPKKSVIDDSSNTTAPTTRKARRERAQAEKGKYFTL
                     NFKDVEINEFLNVMSQLIGKNIIIDEKIRGKVTISSAKKIPVTQAYDIMKSILEVKGL
                     AVIETENLIKIVPIKEAIRKNVEVMIDDNKKKPPHDDDRIITYLLELRYADANEIQRV
                     LMALKPQNTDIVVYKLMNILIFSGTSSDIKGLVRIANELDKKVEDEVIEEKKPKGNIH
                     VVHLENANAEQLANVLSRIPFSDKALIQTGQPTQPQTPERKPVKESSKSSRVTQTQKT
                     GSPTSPQTKLSIIANKETNSLIITATPEEYNDIRRIIKELDIVREQVYIESLIVEVTA
                     ENSWGLGIDWMLGAQSGENLYGGSSIMGNPPSYKLPDGFTGKKLAVPLSTGFQLGYLA
                     DKSVLGYVLLNATGTDKNFNVLSTPQLLTTDNQEAELNVGQEIPVPTNNRISDTGTQF
                     YTYEYKSVGVKFKINPHITQKERITLDLYLEVNSVLGTTQQLPGGSIIPPSMGRRDMK
                     TKISVHDGQTIVVGGLITNTKSDSEVKVPLLGDIPLLGWFFKRKTTEYKKTNLLVFIT
                     PYIVTKKDKIDSITRHKKEEQSRIKLRD"
     gene            2897..4600
                     /gene="gspE"
                     /locus_tag="PKJ16_07665"
     CDS             2897..4600
                     /gene="gspE"
                     /locus_tag="PKJ16_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HNV46902.1"
                     /translation="MQKITKEKHLGTLLVEKGVITNEQLQEVLLAQKGSNMRIGQLLI
                     KKGLATEDEILSALSERFGIAYVKHIEFADPDNIFTKIPVHFLKKNKIVPFKYEGKTI
                     FVAVLDFLNIQPFDDLKMIYQEYNFEPVIVSNDEIQRLLNSHFDVMKNESTDEVIDNL
                     EESDLEILSSPVSETEDILDMANEAPIIKLVNMAMREAINRRASDIHIEPYEKDLIIR
                     FRIDGILYPMYTPPKKFQGAIISRIKIMANLNIAENRLPQDGRIQLKIGGKDIDIRVS
                     IYPTYYGERVVLRLLNKTDMEFSLNSLGFSDDTLEKFNELIKKTHGIVMVTGPTGSGK
                     STTLYSVLTRLNQPDVNILTVEDPIEYQLHGIGQMQVKPKIDLTFAAGLRSILRQDPD
                     IIMVGEIRDVETAEIAVQAALTGHRVFSTLHTNDAASGITRLIDMGVEPFLIASSVNA
                     FLAQRLVRSICPHCKTSFRPTPKMLSDLGLSPAKFKNVKLYQGKGCDKCLNTGFMGRM
                     GIYELLPVSTEIRKLVMAHSDAMQIKELAIKEGMVTLYNDGLEKVMKGITTLEEVIRV
                     S"
     gene            4603..5847
                     /locus_tag="PKJ16_07670"
     CDS             4603..5847
                     /locus_tag="PKJ16_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HNV46903.1"
                     /translation="MIFEYQALNKKGDTVYDFVDAPTDVAARQKLRGQGLYVVKLTKH
                     EVHDRASSGSGIFGKMYADALNFIALKISSKQVGIFSRQLATLLRAGMPLPVAIADII
                     EQIENQNFKKVVVDIREKLEEGSSLSNALTRHRVIFSEMYISMVRVGENLGSLDQVIE
                     RLADLEEKKNILKSKIQAALWYPSFMIFFAIAVVVFLMVNIIPSLSRMFIDLGRELPL
                     PTKIVMGISGFFSTFWWVLIILFVGTFYYLNHYIKTPEGRRKFDEFKLDFPLVSKLYR
                     KLIVLRFTQNLGILLNNKVDIIKSFEIVKKIVMNVIIEERIEDASKRIKEGSSVSNAL
                     SKSGFLPKLVMGMITAGEASDNLDTMLLNIGKVYETEIDLTVTSLTNLIEPFIIIFMG
                     IVIGSIVISVLLPIFEMNLIMQ"
     gene            5907..6389
                     /gene="gspG"
                     /locus_tag="PKJ16_07675"
     CDS             5907..6389
                     /gene="gspG"
                     /locus_tag="PKJ16_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HNV46904.1"
                     /translation="MRRIGTTLKKRLQAVGAALKSNGGFSFIEIMIVVVIIGILSVLV
                     VPRFMGITDKAKVAAAKQQISSFGIALNQYYLDNGNYPTTEQGLTALVQKPGTEPLPQ
                     NYSEKGYLEKKQIPKDPWGRDFIYKSPGEHGNDYEIYCLGADGKEGGEGINAEIKSWE
                     "
     gene            6395..6982
                     /locus_tag="PKJ16_07680"
     CDS             6395..6982
                     /locus_tag="PKJ16_07680"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HNV46905.1"
                     /translation="MLPSPLQRWAARLRADNRGFSLIEIIIVIVIISIMSIVVGPRLT
                     NYMGARRVNFNLLSSIIAKTFDDAYINERVNFLVIHLGQPNPEQIADPGDAIAARKNA
                     VSVVTLGPDGTFVDSANRLLKYKSFPDSFRLEAVHLASGEKITQGNVFIPFYPTARAD
                     DAIIQVLVDGTEHWSVRIFKFKKEPEIKNEQKVFE"
     gene            6979..7374
                     /locus_tag="PKJ16_07685"
     CDS             6979..7374
                     /locus_tag="PKJ16_07685"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HNV46906.1"
                     /translation="MIMRERYAHRSRGFALIEILVAITILSVALLAIISGVSAGIVAI
                     TGNKNLTQAVIIAKTKFNEFQTSKFRGADITDEPVKEYPGYAFSRKTTRFEHAIFGPI
                     SAKKVEIIVSWSDRNRKKSYSISYVYPER"
     gene            7379..8053
                     /locus_tag="PKJ16_07690"
     CDS             7379..8053
                     /locus_tag="PKJ16_07690"
                     /inference="COORDINATES: protein motif:HMM:NF023044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HNV46907.1"
                     /translation="MRRFVEQVKRLAGDSSGFSLIEILVATVVSSIILMMVYSSHRSI
                     MNAVKDLTGVAEFYENINLAITRIDKDISCAYFNKFNKDVCFLGENNFTPPSSGKINF
                     VTVDRNDINIKLDPKKEFRTSDVHEVGYSLRKDRDSDNLYQLVRREDVHYDDDPETGG
                     RESVLLENVVDIRFEFLLLNNWTPSWDSRKYKKFPRAVKTILKLKNYRGNDEEFVFIS
                     YVLMES"
     gene            8053..9336
                     /locus_tag="PKJ16_07695"
     CDS             8053..9336
                     /locus_tag="PKJ16_07695"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
                     /protein_id="HNV46908.1"
                     /translation="MANCDHDASGAGGARSKAARVLIRALRACSGAWRRWREKYERRR
                     QALEDRGVLRYLFGSRGYVLVIVLLITSMMVSVSTEFLIVAQTNINYIKKLNERLKAF
                     TIAKAGMNLATFILEADQKGLAAGLMTQKADKNIDCYLDLWALEFPELPLEYGTLKLK
                     IDDENAKINLSILANEYIDKTPYYFIAQRFFVNMGLPPDIADSILDWVDIDDARSPYG
                     AESSDYYQSLPNPYKCKNKEMDSIDEMLLVRGITPEIFYGLGGGNFGLEQNLVDDNKG
                     KKRLDPSMFAGMAKEAPADKQDLSDIEQKIGKEKSRRLDEYFRVNGERSDYMSDLNKI
                     NINTASYRVLSALTDAMTDDIVTEIIRRRRQKPFSTVDEVKDLVTDENIRKNNLTVKS
                     YYFRIESVGRFNNTTVKIIGIYFRDNKRFFYWSER"
     gene            9393..11030
                     /gene="pilM"
                     /locus_tag="PKJ16_07700"
     CDS             9393..11030
                     /gene="pilM"
                     /locus_tag="PKJ16_07700"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HNV46909.1"
                     /translation="MFEKIAALDIGTSSIKIILVRTGFKDFQVVSFVYEDIDLNVEDH
                     KAAVRDALGRIIRENPLDGYTLVTTLPMESAIIRNLVFPFTDYAKIAQAIPFEAEEII
                     PFKMEHLITHFQKLRGKTPDESRVLLAASHKDIINDRLKLLEEFGLAPVRMGLESNSM
                     YECYRYFNKMPEEAIIQVDIGHSKTVMNIIKDSELLFTRCIPVGIGGVYDTICEKLEI
                     SRNDAVDLLANLNIDLTAFENNLQRDYYKTLKVTKSTLREIFDNLTGSVQEIVEQIVL
                     TNKSFAVDYMPLEFNRILLSGGGSTIQGIGTILSNELGLPVVSLPFLVEYHNDVRVQT
                     QFPVAFGTVISHLNKKSLPVNFLRDEFQPAYGADSHRMYYLAGMFLILSIVVLFVNVV
                     VSSILKTTVNNKYNTILQERFQRYFRMQNPGNDPVATAMKLYQDEKKEFSSLKSVVQA
                     EDSTLEVLKAILTHFPKDPTFELSNIVINERIVRIDGTIASSKNIDDFKNKLSESPRF
                     ESVNLNTNIDKKNQVRFSLTIKLRIADDQQREYKKIR"
     gene            11038..11574
                     /gene="gspM"
                     /locus_tag="PKJ16_07705"
     CDS             11038..11574
                     /gene="gspM"
                     /locus_tag="PKJ16_07705"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HNV46910.1"
                     /translation="MLNPLKNIKLSTREKIMLQALAGVIAVAVLYLLIIAPFIEFRES
                     SDSALAKNVERLKKIDKIYDDYRELKREKSKYDAMLAQKNENITSLIEQWSNSVNIAR
                     NIAYTRRTQANIQNKYVRVTTDVKFEGVSIEKFLKFIYEVENSNRLIKLSYIRINTAL
                     KGTNTYDIVLKIDSFTAQ"
     gene            11594..12553
                     /gene="gspN"
                     /locus_tag="PKJ16_07710"
     CDS             11594..12553
                     /gene="gspN"
                     /locus_tag="PKJ16_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HNV46911.1"
                     /translation="MDIKALQAKVIEAVKSAWILFKEILELPRAKLYLLIAAAMTLLF
                     VVFTFPYDILVRNNIKALEKVTGGTINLGQLDFSLWRNTYIDSVAVYFADGTELSLKD
                     VNVDFTKNPISLFLLNRFSGDFKVNSLKYQKKDMSIEGVVKVLMSSAFDTKKDIITSG
                     SLSIALQNINVKGIEIKGFVIPPIKFSGIAAEAKIANNQLRIDRCVFSGVDLRGQITG
                     TIALNKMMKSSRLNLKIEIDGNSRVLEDYKILLGSLTGSDGDKIRLDVGGMLHSPEVN
                     FPQKTEAPPQKAKPGAEPDDDDEAPRQAPGPGKRPPVDTKKTI"
     gene            12556..14283
                     /gene="ispH"
                     /locus_tag="PKJ16_07715"
     CDS             12556..14283
                     /gene="ispH"
                     /locus_tag="PKJ16_07715"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HNV46912.1"
                     /translation="MKIEIARNSGFCMGVRDAIVRIVGEINNAREPIYVYGPLIHNPQ
                     TMSVLEKRGLITIHDLDHLDGRTIAIRTHGIPLANLKDIRDRSRRYINLTCPRVARVQ
                     GLIKKHSRNGVYTIIAGDQAHAEVEGLMSYAQSGVSVISTPEEIDSIPSAPRYLLISQ
                     TTFDTSLFDRIVERASAKLENLITINTICDSTSNRQHDVISAVRGGIDALVVVGGKNS
                     ANTRRLARIGTDHGIKTFHVETEDELEPDHFRGVTRVLVTAGASTPGWIINRILERLY
                     DISFENRNRLLSRMKKAAELVVRTNLFSAFGALCVTAITQLLAGYPPDASLSLVSFLY
                     IFSVYTINNYFDIDFIKVSNPYKHGVYSRHKRFLLPVAVTLIVISAVLMAIFHAMLPL
                     VIFMASAALGVVYSSKKVKGLIRFAPLAFVRSFFTSKNIISSVGWTVISVIIPLIAID
                     APSVYSLLFGVYVFAFMFFRNTLLDVIALHGDIIIGRETLPVLLGIDRTRALLGIIAL
                     AACAGIGVMAFVGGEAFAILFALPFIYGMVLLQIIIKTGYVVSIKFEMLVEMMFVVLG
                     AASLSLLVR"
     gene            complement(14284..14769)
                     /locus_tag="PKJ16_07720"
     CDS             complement(14284..14769)
                     /locus_tag="PKJ16_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46913.1"
                     /translation="MRFGRAKNVVFQKQSYSTRTSTKLNKAIVIAFALHLGVSAILSC
                     GTPKGTFAFKGALDDRYHTLSETPEFEQGSPAEWAYVFNKNYGLRTFGIVVLKKELVW
                     VEVSRRTASIDKTSRVIYGSLGDLSTGQYKIIITEKATVIDEREFVIYAGEDDEDFDR
                     D"
     gene            complement(14930..>15080)
                     /locus_tag="PKJ16_07725"
     CDS             complement(14930..>15080)
                     /locus_tag="PKJ16_07725"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HNV46914.1"
                     /translation="LNYTNVRDVLRTPGLEELNIGHSIIAQSVFTGLATAVRDMAILV
                     EESNA"
BASE COUNT         3697 a         4152 c         4145 g         3086 t
ORIGIN      
        1 ccggtttggc accgatagaa gcacggtcgt agatgcacat gtgtaacgat ggataacata
       61 cgatatcacg cagtaaatac ggtcgcggtc attatcttcg cctatatcac cgcgattacg
      121 ataaaccagg tcattcgttt cgtgttcgcc ccgacgttct cgataccgca gaaggcgatg
      181 atcgagaaac tcgcgcagcc gcgcctgaag cccttcgagg agtacaagtc catcgtcgat
      241 tccggtttct tcaagattcc gtcggagact ccggacgtgc cgggcgtcgg ccaggggggc
      301 gctggggccg agagcgtaaa cgagctgctg ctgttgggga ccatcaccgg ccccagccac
      361 atcgcccgcg cgctcatcaa gaagaaaacc gaaaaggacc cgcagatttt ccgtctttac
      421 ggcacggtcc atggccataa gcttataaga attgacaact cgaaggtgta tcttaaaacg
      481 ggaaacagcg tgcagatcct tgacatgttc gccaaagagg gcgcggcggc gactcccgcg
      541 aaagcgccgt ccggcaccgg ggccgcgcta cccacctcgg gcaaggtggt gcaaaacctc
      601 agcaaggccg agctgcagca aaaggtgctc aacaacgtcg acaacgcgct ccagggcatc
      661 cgggccggtc cctaccgcgt agacggcgcc atcaaagggt ataagatctt tcgcatcaag
      721 ccccataaca tcatgtacaa gctgggggcg cgaaacggcg acgtgctgat gcgggtgaac
      781 gggcatgcga tcgacagcac ggagaagctc ttgagcatct ggaagtcggt ccagggcgat
      841 tcgaagatct cggttgacct ggagcgcggc ggcaagctct tgacgttcga tttcaatatt
      901 accgaataaa cgcatgggaa aggcatgatg atatctacga cgggagtcgc gcgcatcgca
      961 ggaattctgc ttatcggcac cgttattttc tcggtcggcg acgttcggac gcaggaggcg
     1021 ccggtcaagg aacccacgcg cgaggagagc aagcaaccgg tacgtaccga agccccggcc
     1081 gccacggaca aaaagacgac cccaaccata aagaatgacg ctacgccgaa aaagtctgtt
     1141 atcgacgatt cgtcgaacac gacggctccg acgacccgaa aagcgcggcg cgaacgcgcc
     1201 caggcggaaa agggcaaata tttcaccctg aacttcaagg acgtcgagat caacgaattt
     1261 ctcaacgtca tgagtcagct catcggcaag aacatcatca tcgacgagaa aatccgcggg
     1321 aaggtcacca tcagctccgc gaagaagatc ccggtgaccc aagcgtatga tatcatgaag
     1381 tcgatcctcg aggtgaaggg ccttgcggtc atcgagaccg aaaatctcat caagatcgtc
     1441 ccaatcaagg aagccatccg taaaaacgtc gaggtgatga tcgacgacaa taaaaaaaag
     1501 ccgccccacg acgatgaccg catcatcacc taccttctgg agctccggta tgcggacgcg
     1561 aacgagatcc agcgcgtgct gatggcgctc aaaccgcaga acaccgacat cgtcgtttac
     1621 aagctgatga atatcctcat cttcagcggc acctcctcgg acatcaaggg actggtccgg
     1681 atcgcaaacg agctggacaa gaaggtcgag gacgaggtga tcgaggagaa gaagccgaag
     1741 gggaatatcc acgtcgtcca cctggaaaac gcaaacgccg agcagctggc caatgttctc
     1801 tcacggattc cattctccga taaggcgctc atccagaccg ggcagccgac acaaccgcag
     1861 acgccggaac gcaaaccggt gaaggagtcg tcgaaatcga gccgggtcac ccagacgcag
     1921 aagacgggct cgcccacatc gccgcaaacc aagctctcca tcatcgcgaa caaggaaacc
     1981 aactcgctca tcattaccgc gacgccggag gagtacaacg atatccgccg catcatcaag
     2041 gagctcgaca tcgttcgcga gcaggtgtat atcgaatcgc tgatcgtcga ggtcactgcg
     2101 gaaaacagct gggggctggg catcgactgg atgctgggcg cccagagcgg ggaaaatctc
     2161 tatggcggtt cgtcgatcat gggcaacccg ccgagctaca agcttccgga cgggtttacc
     2221 gggaaaaagc tcgccgtgcc gctgtcgacc ggtttccagc tgggctacct tgcagacaag
     2281 tcggtgctgg gatatgttct cctgaacgcg accgggaccg acaagaactt caacgtcctt
     2341 tcgacaccgc agctcctgac gaccgacaat caggaggcgg agctcaatgt cgggcaggag
     2401 atcccggtcc cgaccaacaa ccgcatcagc gatacgggga cgcagttcta cacctacgag
     2461 tacaagtccg tgggcgtgaa attcaagatc aatccgcaca tcacccagaa ggagcggatc
     2521 accctcgatc tgtatcttga ggtgaattcg gtgctcggca ccacgcaaca gcttcccggc
     2581 ggctccatca tcccgccgtc gatggggcgg cgcgacatga aaacgaagat ctccgtccac
     2641 gacgggcaga cgatcgtcgt cggcgggctc atcaccaata ccaagagcga tagcgaggtg
     2701 aaggtgccgc tcctgggcga catccccctg ctcggatggt ttttcaagcg caagacaacc
     2761 gagtacaaga aaacgaacct gctcgttttt attacgccgt atatcgtgac caagaaggac
     2821 aagatcgatt ccatcacccg tcacaagaag gaagagcaga gccgcattaa actccgggac
     2881 tagtccctga cgccccatgc agaagatcac gaaagaaaag cacctgggca ccctccttgt
     2941 cgagaagggc gtcatcacca acgagcagct ccaggaggtg ctgctcgcgc agaaagggtc
     3001 gaacatgcgg atcgggcagc tcctcatcaa gaaggggctg gccaccgagg acgagatcct
     3061 ttcggcgctc tccgagcgct tcggcatcgc ctacgtcaag cacatcgagt tcgccgatcc
     3121 cgacaatata ttcacaaaga tccccgttca cttcctgaag aagaacaaga tcgtcccctt
     3181 caaatacgag gggaagacga ttttcgtggc cgtgctcgac ttcctgaaca ttcagccatt
     3241 cgacgatctc aaaatgattt atcaggaata caatttcgag ccggtgatcg tgtcgaacga
     3301 cgagatccag cgcctattga acagccactt cgacgtgatg aagaacgagt cgaccgacga
     3361 ggtaatcgac aatctcgagg agtccgacct cgagatcctg agctcgccgg tgtcggagac
     3421 cgaagacatc ctcgacatgg ccaacgaggc gcccatcatc aagctggtga atatggcgat
     3481 gcgcgaggcc atcaaccgcc gggcgagcga tatccacatc gagccttacg agaaggacct
     3541 catcatccgc ttcaggatcg acggcatcct gtacccgatg tatacgccgc cgaaaaaatt
     3601 ccagggcgcc atcatatcgc gaatcaagat catggcgaac ctcaacatcg ccgaaaacag
     3661 actcccgcag gacggtcgca tacagctcaa gatcggcgga aaggacatcg atatccgcgt
     3721 ttccatctac ccgacctatt acggcgagcg cgtggtgctc cggttgctca acaagaccga
     3781 catggagttt tcactcaatt cactgggctt ctccgacgac accctggaga aattcaacga
     3841 gctcatcaag aaaacgcacg gcatcgtgat ggtgaccggt ccgaccggaa gcggaaaatc
     3901 gaccacgctc tacagcgtgc tcacgaggct caaccagccc gatgtgaaca tactcacggt
     3961 cgaggacccg atcgagtacc agctccacgg cattggccag atgcaggtga agcccaagat
     4021 cgacctcacc ttcgcggcgg gccttcggtc cattcttcgc caggatcccg acatcatcat
     4081 ggtgggcgaa attcgggacg tggaaacggc tgagatcgcg gtacaggcgg cgctcacggg
     4141 ccaccgcgtg ttttcgacgc tccacaccaa cgacgccgcg agcgggatca cgcgcctcat
     4201 cgacatgggc gtcgagccgt ttctgatcgc ctcgtcggtg aacgcgtttc tcgcccaacg
     4261 gctggtgcgc tccatttgtc cgcattgcaa gacgtcgttc cgtcccaccc cgaagatgct
     4321 ttcggatctg ggactttctc ccgccaagtt caagaatgtg aagttgtatc agggcaaggg
     4381 atgcgacaag tgcctcaata ccggtttcat gggacgaatg ggcatctacg aactcctgcc
     4441 ggtgagcacc gagatccgga agctcgtcat ggcgcattcc gatgcgatgc agatcaagga
     4501 gcttgcgatc aaagagggca tggtgacgct gtacaatgac ggcctcgaaa aggtcatgaa
     4561 ggggatcacc accctcgaag aggttatccg ggttagctga cgatgatttt cgagtatcag
     4621 gcgctgaaca agaagggcga cactgtctac gatttcgtgg atgcgccgac cgatgtcgcg
     4681 gcccggcaaa agctccgcgg gcagggactg tacgtcgtca agctcacgaa gcacgaggtc
     4741 cacgaccgcg cgtcatcggg gagcggcatc ttcgggaaga tgtatgccga tgcgctcaat
     4801 ttcatcgccc tgaagattag ttcgaagcag gtggggattt tctcgcggca actcgcgacc
     4861 ctcctgcgcg cgggcatgcc ccttcccgtc gccatcgccg acatcatcga acagattgaa
     4921 aaccagaatt tcaagaaggt ggtggtcgat atccgcgaga agctcgagga gggctcgtcg
     4981 ctgtcgaacg ccctcacccg ccaccgcgtg atcttttcgg aaatgtatat cagcatggtg
     5041 cgcgtgggcg agaacctcgg ctcactcgac caggtgatcg agcgactggc cgatctcgaa
     5101 gagaaaaaga acatcctgaa gagtaaaatt caggccgcgc tctggtatcc gtcgttcatg
     5161 atcttcttcg ccatagccgt cgtcgtcttc ctgatggtga acatcatccc gtcgctcagc
     5221 cgcatgttca tcgacctggg gcgcgagctg ccactgccga ccaagatcgt gatgggaatc
     5281 agcggattct tttcgacctt ctggtgggtg ctcattatcc tgttcgtcgg gaccttttac
     5341 tatctgaatc attacatcaa aacgcccgag gggcggcgaa aattcgacga gtttaagctg
     5401 gacttcccgc tcgtgagcaa gctctaccgg aagctcattg tgctcaggtt cactcaaaac
     5461 ctggggatcc tattgaacaa caaggttgac atcatcaaat cgttcgagat cgtaaaaaag
     5521 atcgtgatga atgtgatcat cgaggagcgg atcgaggacg catcgaagcg tattaaagaa
     5581 ggaagctcgg tatcgaacgc gctctcgaag tcgggatttc ttccgaagtt ggtgatgggg
     5641 atgatcacgg ccggcgaggc gagcgacaat ctcgacacga tgcttctgaa catcggcaag
     5701 gtctacgaaa ccgagatcga cctgacggtc accagcctca ccaacctgat cgagccgttc
     5761 atcatcatat tcatgggtat cgtgatcggg agcatcgtga tatcggtcct gcttccgatc
     5821 ttcgagatga atctgatcat gcagtagtga atcacgaccc ggtgacgcga cggccggcat
     5881 cgatactgaa tacagggagg atgtacatgc gaagaattgg aacgacgctg aaaaagaggc
     5941 tgcaggccgt cggggccgcg cttaaatcga acggtggttt ttcgttcatc gagatcatga
     6001 tcgtggtcgt catcatcggc atcctgtcgg tgctggtggt cccgcgtttc atggggataa
     6061 ccgacaaggc gaaggtcgca gcggcgaaac agcagatttc atcattcggt atcgcgctca
     6121 accagtatta tctcgacaac ggaaactatc cgacgaccga gcagggcctc accgcgctgg
     6181 tgcagaagcc cggaacggag ccactgcccc aaaactacag cgaaaaaggc taccttgaga
     6241 agaagcagat ccccaaggac ccatgggggc gcgacttcat ctacaagtcc cccggcgagc
     6301 acggcaatga ctacgagatc tactgcctgg gggccgacgg caaggaaggc ggcgaaggca
     6361 taaacgccga gataaaaagc tgggaatagc gcgggtgctc ccctcccctc tacagcgttg
     6421 ggcggcgagg ctccgtgctg acaacagggg attttcgctg atagagatta tcatcgtcat
     6481 cgtgataatc tctatcatgt cgatcgttgt cggtccgcga ctcaccaatt acatgggtgc
     6541 gcggagggtg aatttcaatc tgctgagctc catcatcgcc aagaccttcg atgacgccta
     6601 catcaatgag cgcgtcaact tcctggtgat ccatcttggc cagccgaatc ccgagcagat
     6661 cgcggacccc ggtgacgcga tcgccgcgcg gaaaaacgcc gtgtcggtag tcacgctcgg
     6721 ccccgacggc acgttcgtcg acagcgcgaa caggctcctc aagtataaat cgtttccgga
     6781 cagtttcaga ttggaggcgg tgcacctcgc gagcggcgag aagatcaccc agggaaacgt
     6841 cttcatcccc ttttatccga cagcccgggc ggacgacgcg attatccagg tgctcgttga
     6901 cggcaccgaa cactggtcgg ttcgaatatt taaattcaag aaggagccgg agataaaaaa
     6961 cgagcagaag gtgttcgaat gatcatgcgc gagcgctacg cacatcgctc ccgcggtttc
     7021 gcgctgatcg agatactggt cgccattacc attctctcgg tggcgctcct tgcgatcatc
     7081 tccggggtgt cggccgggat cgtcgcgatt accgggaaca agaacctcac gcaggcggtg
     7141 atcatcgcca agacgaaatt caacgaattc cagacaagca agttccgcgg cgcagacatc
     7201 accgacgaac cggtgaagga gtatcccggc tacgccttca gccggaagac cacccgcttc
     7261 gagcatgcga tcttcgggcc gatatcggca aagaaggtcg agatcatcgt gtcgtggtcg
     7321 gaccgcaacc ggaaaaagag ctattccata tcgtatgtgt atcccgagcg atagccgcat
     7381 gagacgattc gtcgaacagg tgaaacgact tgccggggac tccagcggat tctcgctcat
     7441 cgaaatactc gtcgcgaccg tcgtgtcgtc catcatcctc atgatggtgt actcgtcgca
     7501 ccgctccatc atgaacgccg tgaaggacct gacgggcgtt gcggaattct atgaaaatat
     7561 caacctcgcc attacgcgca tcgacaagga tatctcgtgc gcttatttca acaagttcaa
     7621 caaggacgtt tgttttttag gagagaacaa cttcacgcca ccgagcagcg ggaagatcaa
     7681 tttcgtgacg gtcgatagaa acgacatcaa catcaaactt gaccccaaaa aggagtttcg
     7741 caccagcgac gtgcacgagg tcggatactc gctgcgaaag gaccgcgatt cggacaacct
     7801 ctaccagctc gtgcgtcgcg aggacgtcca ctacgacgac gatccggaaa ccggcgggcg
     7861 ggagagcgtt ctgctcgaaa atgtcgtcga catacggttc gaatttttgc tcctgaacaa
     7921 ttggaccccg tcctgggact cgcgcaagta taagaagttc ccccgtgcgg tgaagactat
     7981 actcaagctg aagaactatc gcggaaacga cgaggaattc gtctttatct cgtatgtgct
     8041 gatggaaagc tgatggcgaa ctgcgatcac gatgcctcgg gtgccggtgg ggcgcgttcg
     8101 aaagccgcgc gcgtgctgat ccgcgcactg cgcgcctgct ccggggcgtg gcgccggtgg
     8161 cgtgagaaat acgaacggcg tcgacaggcg ctcgaggatc gtggcgtgtt gaggtatctg
     8221 ttcggttcgc gcggctatgt cctcgtcatc gtcctgctga tcacctcgat gatggtttcc
     8281 gtgtccacgg agttcctcat tgtcgcacag acgaacatca actacattaa aaaactgaac
     8341 gagcggctca aggccttcac catcgcgaag gcggggatga acctcgcgac cttcattctg
     8401 gaagcagacc agaagggcct cgccgccggc ctgatgacac agaaggccga caagaacatc
     8461 gattgctacc tggacctctg ggcgctggaa ttccccgagc ttcccctgga atacggaacg
     8521 ctgaagctga aaattgacga tgagaacgcg aaaatcaatc tgagcatcct ggcgaacgag
     8581 tatatcgaca aaacaccgta ctatttcatc gcccagcgct ttttcgtgaa catggggctg
     8641 ccgccggata tcgccgattc gattttggac tgggtcgata tcgacgatgc gcgttcgccc
     8701 tatggggcgg aatcatcgga ttattatcag tcactgccga acccctacaa gtgcaaaaac
     8761 aaggagatgg acagcatcga tgagatgctg ctggtgcgcg ggattacgcc ggaaatattc
     8821 tatggattgg gcggcggcaa cttcggactt gaacaaaacc ttgtcgatga caataaaggc
     8881 aagaagcgac tcgacccgtc gatgtttgcc ggcatggcga aggaggcgcc tgcggacaag
     8941 caggacctat cggacatcga gcagaagatc ggcaaggaaa agtcgcggcg tctcgatgag
     9001 tattttcggg tgaacggcga gcggtcggat tacatgagcg acctgaataa aattaatatc
     9061 aataccgcca gttaccgcgt attatcggcg ttgacagacg ccatgacgga tgatattgtg
     9121 acggaaatca tccggcgaag gcgacagaag cccttctcga ccgtcgacga ggtgaaagac
     9181 cttgtcacgg acgaaaacat ccggaagaac aacctgaccg tgaagtcgta ctatttcagg
     9241 atcgagtccg tcggcaggtt caacaatacg acggtgaaaa tcatcggtat ttattttcgg
     9301 gacaataagc ggttcttcta ttggagcgag cgatgatccg gcatcgatgt cgcggccggt
     9361 aaccggggaa ccgaacggaa tggagcacct cgttgtttga gaagatcgcg gcactagaca
     9421 tcggcacatc gtcgatcaag ataattctgg ttcggaccgg tttcaaggat ttccaggtcg
     9481 tttcgttcgt ctatgaggac atcgacctga acgtcgagga ccacaaggcc gccgtcaggg
     9541 acgcgctcgg gcggatcata cgggaaaacc cactcgacgg ttatacgctg gtcacgaccc
     9601 tccccatgga aagcgcgatc atccgcaacc tcgtctttcc gttcaccgac tacgcgaaga
     9661 tcgcccaggc gatacccttc gaggcggaag agatcatccc cttcaagatg gagcacctga
     9721 tcacgcactt ccagaaattg cgcgggaaga cgcccgacga atcgcgcgtg ttgctcgcgg
     9781 catcgcacaa ggacatcatc aacgatcgcc tgaagctgct ggaggaattc ggcctcgcgc
     9841 cggtgcggat ggggttggag tcgaactcga tgtatgagtg ttatcgttac ttcaataaga
     9901 tgcccgaaga ggcgatcatc caggtcgaca tcggccacag caagacggtg atgaacatca
     9961 tcaaggacag cgagctgctg tttacgcgct gcatcccggt gggcatcggc ggcgtctacg
    10021 acacgatatg cgagaaactg gagatttccc gaaacgacgc ggtcgatctt ttggcgaatc
    10081 taaatatcga cctgacggcg ttcgagaaca atctgcagcg cgattattac aaaacactga
    10141 aggtcacgaa atcgacgctg cgcgagatct ttgataatct gacgggctcc gtacaggaaa
    10201 ttgtggagca gatcgtcctc accaataaat ccttcgcggt cgactatatg cccctggagt
    10261 ttaaccggat actcctgtcc ggaggtggct ctacgataca gggaatcgga accatcctgt
    10321 caaacgagct cggcctgccg gtcgtttcgc ttccgtttct ggtggaatac cacaatgacg
    10381 tacgcgtgca gacgcaattc ccggtcgctt tcggcacggt gatatcgcat ctcaacaaga
    10441 aatcgcttcc ggtgaacttt ctccgtgacg aattccagcc tgcgtacggc gccgattcgc
    10501 accggatgta ctaccttgcg gggatgtttc tcatcctctc gatcgtggtg ctgtttgtca
    10561 acgttgtcgt ctcctcaata ttgaaaacaa cggtaaacaa taaatacaat acgatcctcc
    10621 aggaacgctt tcagcggtat ttcaggatgc agaaccccgg aaacgatccg gtcgcgacag
    10681 ccatgaagct gtatcaggac gaaaaaaagg aattctccag cctcaagtcg gttgtccagg
    10741 cggaggacag cacgctcgag gtgctcaagg cgatcctgac gcactttccc aaggatccga
    10801 ccttcgagct ttcgaatatc gtcatcaacg agcggattgt gcgtattgac ggcacgattg
    10861 catcgagcaa gaacatcgac gacttcaaga acaagctttc cgagtcgccc cggttcgaat
    10921 cggtcaatct gaacacgaat atcgacaaga aaaaccaggt gcgtttttcg ttgacgatta
    10981 aactgcgcat tgccgacgac cagcaacgcg agtacaagaa gatccggtga tcgacgcatg
    11041 ttgaatccac tgaaaaacat caaactgtcc acccgcgaaa agatcatgct gcaggcgctt
    11101 gccggcgtga ttgcggtcgc ggtactgtat ctgctcatca tcgcgccctt tatcgagttt
    11161 cgcgagagca gcgatagcgc cctcgcaaag aacgtcgagc ggctgaagaa aatcgacaag
    11221 atttacgacg actatcgaga gctcaagcgc gaaaaatcga agtacgatgc gatgctcgcg
    11281 caaaagaacg aaaacatcac ctcgctcatc gagcagtggt ccaacagcgt gaacatcgcg
    11341 cgcaatatcg catacacgcg tcgtacgcag gccaacatcc agaacaaata tgtccgggtg
    11401 acgaccgatg tgaaattcga gggcgtttca atagaaaagt tcctaaaatt tatatacgag
    11461 gtcgagaatt cgaatcgact aatcaagttg agctacattc gcatcaacac ggccctcaag
    11521 gggaccaata cctacgatat cgtcctcaag atcgacagtt tcaccgcgca atagcattgg
    11581 aaccggatac ggcatggata taaaagcact acaggcaaaa gtgatcgagg ccgtgaaatc
    11641 ggcgtggata ttattcaagg agatactcga gctgccgcgg gcgaaactct atctgctgat
    11701 cgcggccgcg atgacgctcc tgtttgtcgt gttcaccttc ccgtatgata ttctggtgcg
    11761 aaacaacatc aaggcgctgg aaaaggttac cggcggcacg atcaatctcg ggcagctcga
    11821 ttttagcctg tggcggaaca cctacatcga ttcagttgcg gtatatttcg cagacggcac
    11881 cgagctttct ctcaaggatg ttaatgtcga ttttacaaag aatccgattt cgctgtttct
    11941 gctgaatcga ttctcgggcg atttcaaggt gaactcgctc aagtaccaga aaaaagacat
    12001 gtcgatcgaa ggcgtcgtca aggtcctgat gagctcggcc ttcgacacga agaaggacat
    12061 cataacgagc gggtcgctgt ccattgcgct ccagaacatc aacgtgaagg ggatcgagat
    12121 caagggattt gtgataccgc cgattaagtt ctccgggatc gcggccgagg cgaagatcgc
    12181 gaacaaccag cttcggatcg accggtgcgt tttttccggc gtcgatttgc gcggtcagat
    12241 aaccggcacg atcgccctca acaagatgat gaaatcttcc cggctcaatc tgaaaatcga
    12301 aatcgacggc aactcgcgcg tactagaaga ctacaaaata ctccttggca gcctgaccgg
    12361 ttcagacggc gacaagatcc ggctcgatgt gggcgggatg ctgcatagtc cagaggtcaa
    12421 tttcccacag aaaacagagg cccctcccca aaaggccaaa cccggcgcgg agccggacga
    12481 tgacgatgaa gccccccgac aggcgccggg tccgggaaag cgcccaccgg tggacacaaa
    12541 gaaaaccatt tagcgatgaa aatcgaaatc gcacggaact ccggcttttg catgggggtg
    12601 cgcgatgcga ttgtgcgcat tgtcggtgag atcaacaatg ctcgggagcc catttatgtg
    12661 tatggtcccc tcatccacaa tccgcagacc atgtctgttc ttgaaaaacg cgggctcatc
    12721 accatccacg atcttgacca tctcgatggg aggaccattg ccatacggac tcacgggatc
    12781 ccgctggcta accttaaaga tatcagggac cgttcccgaa gatatatcaa cctgacctgt
    12841 ccccgcgtcg cccgcgtgca aggcttgatc aagaagcatt cgcgcaatgg cgtgtacacc
    12901 atcatcgccg gagatcaagc gcacgccgag gtggaggggt tgatgagtta tgcccaatcg
    12961 ggcgtgtcgg tcatctccac tccggaggag atcgattcga tcccctccgc accgcgctac
    13021 ctgcttattt cccagacgac gttcgatacg tcgctcttcg ataggattgt cgaacgtgcg
    13081 agcgccaagc tggaaaacct catcacgata aatacaattt gcgactcgac cagcaatcgg
    13141 cagcacgatg ttatctctgc ggtgcgggga ggaatcgacg ctctggttgt cgtgggcggc
    13201 aagaattcag cgaacacacg gcgcttggcg cgcatcggaa ccgaccatgg catcaaaacg
    13261 ttccacgttg agaccgagga tgaacttgaa ccggaccatt tccggggcgt gacgcgcgtg
    13321 ctcgtgacgg cgggtgcctc aacgcccggg tggatcatca atcgcatatt ggagcgcctg
    13381 tacgacatca gttttgaaaa caggaacagg ctcctgtcgc gcatgaaaaa ggccgccgag
    13441 ctggtggtgc gcaccaacct cttctcggcc tttggtgcgt tgtgcgttac cgcgataacg
    13501 cagctgcttg cgggctatcc tccggacgcg tcgctttcgc tcgtctcttt tctgtatatt
    13561 ttttcggtat acacgatcaa taattatttc gacatcgatt tcatcaaggt gagcaatccg
    13621 tacaagcatg gggtatacag cagacataag cgctttctgc tgccggttgc cgttacgctg
    13681 atagtcattt cggcggtact catggcaatt ttccacgcga tgcttccgtt ggtcatcttt
    13741 atggcgtcgg ccgcgctggg cgttgtgtat tcgtcgaaga aggtgaaagg gctgatccgg
    13801 tttgcgccgc tggcgttcgt tcggtcgttt ttcacatcga agaacatcat ttcgtcggtg
    13861 ggctggacgg tcatatcggt gatcataccg ctgatcgcca tcgatgcgcc gtcggtctat
    13921 tcgctgctct tcggggttta cgtattcgcg tttatgttct tcagaaacac cctgctcgat
    13981 gtcatcgcat tgcacggtga catcattatc ggccgggaga cacttcccgt gttgctcggg
    14041 atcgacagga ctcgggcgct gctcggtatc atcgcgcttg ccgcatgcgc cggtatcggg
    14101 gtgatggcat tcgttggcgg cgaggcattc gcgattctct tcgcgcttcc gttcatctat
    14161 ggcatggtgt tgttgcagat cattattaaa accgggtatg tcgtttcgat aaaattcgaa
    14221 atgcttgttg agatgatgtt tgtcgtgctg ggagcggcgt ccctttcgtt gctcgttcgg
    14281 tgatcagtcg cgatcgaaat cttcatcatc ctcgccggca tagatgacga attcgcgctc
    14341 gtcgatcacc gttgcctttt ccgtgatgat gatcttgtac tggccggttg aaagatcgcc
    14401 aagcgagccg taaatgacac gagacgtttt atcgatggat gccgtgcgtc gacttacctc
    14461 gacccatacg agctcttttt ttaagaccac gatgccaaag gtccgaagac catagttttt
    14521 attgaaaaca taggcccatt cggcggggct tccctgttcg aactcgggcg tttccgagag
    14581 cgtatgatag cggtcatcca gtgcaccctt gaacgcgaag gttcccttcg gcgttccgca
    14641 ggatagaatt gccgataccc caagatgcag ggcgaatgca atgacgattg ccttattcaa
    14701 ctttgtactg gtccttgtcg agtacgattg tttttggaac actacgttct tcgcgcgtcc
    14761 gaaacgcatt ccaccgcggc tcctgttggt ccccatcgac acgtttcgga cgcgcaggga
    14821 aaaaaatcaa cttgatggcg ctatacagta catatacaag catggcgatg ataagtatct
    14881 tgaaaaaccc aaggatcatg ccagtctccg ttcaatattt cggcacggcc tacgcatttg
    14941 attcttcgac gagtatggcc atgtcgcgta ctgcggtcgc aaggccggta aaaactgatt
    15001 gcgcgatgat ggaatgcccg atgttgagct cttccagtcc cggcgtgcga aggacatcac
    15061 ggacattggt gtaattgagg
//
LOCUS       DAORHF010000069        15078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3014_length_15078_cov_5.717300, whole genome shotgun sequence.
ACCESSION   DAORHF010000069 DAORHF010000000
VERSION     DAORHF010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15078
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3014_length_15078_cov_5.717300"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="PKJ16_07730"
     CDS             complement(<1..357)
                     /locus_tag="PKJ16_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV46915.1"
                     /translation="MNRFGVLLKAGREALSLTIRDVEASTGISNAYLSQMENGYVKRP
                     ALEKLKLLGDLYHIPYEVLMTHTGFPLPDYGLLPNADQERHAILIIDDSANDRELYRT
                     YLENARDVNFEIHQAAS"
     gene            579..746
                     /locus_tag="PKJ16_07735"
                     /pseudo
     CDS             579..746
                     /locus_tag="PKJ16_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            complement(1273..1485)
                     /locus_tag="PKJ16_07740"
     CDS             complement(1273..1485)
                     /locus_tag="PKJ16_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46916.1"
                     /translation="MILQAIKKDGGFFIPDVEGIPSDQSKISVDLVIVDTIDEKKKKL
                     FNTAGILKEKNIDGVEYQKFIRNEWE"
     gene            2200..3375
                     /locus_tag="PKJ16_07745"
     CDS             2200..3375
                     /locus_tag="PKJ16_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HNV46917.1"
                     /translation="MEAAFADRITDVPRSFIREILKVTLDRSVISFAGGLPNRDLFPI
                     AELKAAAMRVFDEEGADALQYGPSEGHAPLRDYISRRYRERLGLEIPPERILITSGSQ
                     QGLDLLAKTFLNEGDDILIEEPGYLGAIQAFSVYRARFVPVPLTESGMDIERLTGAMA
                     SSRPKLAYMVPNFQNPSGISYSAENRRAVAEAIGGTRTIIIEDDPYGELRFDGTPAPS
                     FGALAPENTILLGSFSKTVVPGFRLGWIAAPPAVMDRLLVAKQASDLHTNSFSQRVLA
                     RYLLDADPDAHIARIIAVYREQRDAMLRAIDGHFPGSVRRTRPQGGMFLWATLPEGAS
                     SLALFEKAAARKVVFVPGTPFYIGRDDAPTMRLNFSSVDAATIAEGIRRLGDAMREG"
     gene            complement(3401..4297)
                     /locus_tag="PKJ16_07750"
     CDS             complement(3401..4297)
                     /locus_tag="PKJ16_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV46918.1"
                     /translation="MRFNELNLMRMSLALSVVYACVSLPIAVLCDSMTVLLDALYCVA
                     DVIISLFAIYTVRRLGEPPNERYHFGYAKYEPVQTTLNGVLITALCVAAIIGSIQDIV
                     HHDPVARLSLAIWYAGIGMFVCTGFGAFMRIMGKRLNSDILAANGVLWIIDGLLDLCV
                     FVAFVAIASLKGVVDERFLNYVDPVMCIALSCVLMRKPLLILRDGFLDIVDASPGEDM
                     RLRIDELLSGYLARHRIGATRHIRIRKAGRKLFVVIGLAAAGHRPLREITAIRREIEQ
                     GIAASLSGAEVSVAFEEQTRRS"
     gene            4589..7087
                     /locus_tag="PKJ16_07755"
     CDS             4589..7087
                     /locus_tag="PKJ16_07755"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV46919.1"
                     /translation="MIRKKITLIALVILALGLAGYGGLRLAFRCSVDPPTGAITLPGI
                     TGTVVIRRDALGIPLIEAANEPDLYFATGYAQASDRLWQMVFMRMVMQGRLAEIIGAG
                     GLDFDIAMRSLHARRYVDEAMARLRPGTRALLEAYSRGVNAYIETHRILPPEFILTRH
                     RPEPWKPEDCFYVFGLLNMSVSGNLIEELGFLLAANRLGYGKAAVLFPVYPDEPLPLA
                     EARKLAGIETRDLAAPLDRLSASLRSLTNVMPMNKPASNNWAVAGSRTKSGRPIVTND
                     THLDLMIPNSWMIMHLKCPTLDVAGVMAPGLPIVCCGFNGSVAWGATMVMADSQDLFL
                     EKLKTDGGSRSYLYKGAWRPVSMRTETFRVAGGETVQREFASTIHGPLFNEALERMPF
                     PPEMPVQTLPMKSSYGLALSWALEGGSDSLEGFLALGRARTMAEARAALLRVKSIYLN
                     IVYGDRTGIAWQVTGLFPTRRAGTGQFPSPGWDGAHDWTGFVPMERNPSSLNPAEGYI
                     GTANNRTVSANHPFVMSTSWFGPDRADRIAQVLSGTRTATAESMHALHFDRYSRLAAK
                     MQRLLSDGPLAERVRASIARLSDEEKRRAATQALAMLAPTAFDCVMRPESANAALYGA
                     FVHTFTRATFLDELGPDDSVVWESFVHTNMISYSAIEDHLQGREDSPFWNDVGSGGRE
                     DKADILAESLARAYALCGERMGGDPKKWQWGALHTYHWRHEFAKKLPILHGYFNRGPH
                     PAGGDGQTVNVAAWAPGKNFDTWLIPAMRLVVDFGLADPAFLVTVPGQSGNPSSVHYG
                     DMIEHYLTGKNHPLPFATKAIDAQYADRLELKPR"
     gene            7131..7739
                     /locus_tag="PKJ16_07760"
     CDS             7131..7739
                     /locus_tag="PKJ16_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46920.1"
                     /translation="MNAKKEIRRGLPIGIIIVLLLGCTPAAQKVAPGDYRITRPGATS
                     VVVVHSRSGNTALVAKLLADAAGATYVRLKAPEGTGDSLISSPNRNAEVAIEPRTVDL
                     SAYRTVFLGTPIWFWHPAAPICSFIRANDLTGKRVVLFYTYQGGLAKTAIDEWKALVA
                     KRGGTVVEVIGINRKMHATDEALAAEVTSIIAGRLKSLLPSK"
     gene            7886..10342
                     /locus_tag="PKJ16_07765"
     CDS             7886..10342
                     /locus_tag="PKJ16_07765"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV46921.1"
                     /translation="MADTITRDQRPERGGSLADSIKRHIVLMLGNDFHPPRKDTYYNG
                     LAYCVRDRLIERWLSAQRLYYDTHSKRVYYLSMEFLPGRFMKNYLINLEMEDEVTAAL
                     EETGFTIDELAEEEPDPGLGNGGLGRLASCYLDSMSRLGIPGYGCGIRYDYGIFHQGI
                     VDGYQVEECDNWLQYGNPWEIARRNFMYTVKFGGRSEPYTDDTGRVRYRWVGTDNVNA
                     MACDILIPGYGNYTVNNMRLWAAVSSRDFNLDFFNHGDYIRAMQDKVLTENISKVLYP
                     SDEVEQGRELRLKQQYFFVAATFQDIIRRFRKEHADHRTLPDRVAVQLNDTHPSIAIP
                     ELMRILLDEEALDWDEAWDICVRTFAYTNHTVLPEALETWPVALMGRLLPRHLEIIYE
                     INRRFLEEVRRRHPGNDALAAKLSLVQEHPEKRVRMAHLAIVGSHSVNGVAELHSRIL
                     TTSLFREFHEFFPGRFTNVTNGITQRRWLLQANPALARLITGAIGPGWINELSELKKL
                     VPLAEDASFRAAWREVKRANKERLAAYIAKATGITVNPDSLFDIQIKRIHEYKRQLLN
                     VLHAITLYNRIRTNPNDHFAPRTVIIGGKAAPAYAMAKLIIKLVNAVAETVNRDPAVG
                     DRLRVAFLPNYNVSLAERVMPAADLSEQISTAGMEASGTGNMKFSLNGALTIGTLDGA
                     NIEIMEEVGRENIFIFGLRIEEVERLRTTGYDPREHYRSDAELRQTLDLIASGYFSPE
                     RPDLFAPLVRALLDGGDRYFLCADFRSYVDEQDKVRALYRDPEAWSRTSIINVANMGK
                     FSSDRAVREYAERIWGIGIT"
     gene            10375..10737
                     /locus_tag="PKJ16_07770"
     CDS             10375..10737
                     /locus_tag="PKJ16_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HNV46922.1"
                     /translation="MDESTPIANLKKMALEFRDARNWRQFHDLRNLATGLSIESAELQ
                     EIFLWKNDADIARIAQDESVMRRLREEMADIFIYLLYLSEATGIDLADAVADKIELNG
                     KKYPVEKSYNSSKKYTEL"
     gene            complement(10746..11045)
                     /locus_tag="PKJ16_07775"
     CDS             complement(10746..11045)
                     /locus_tag="PKJ16_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46923.1"
                     /translation="MSPRARRCAHARRYRQIFTPEGVEHDVVPATLQPRSLQSFLSFA
                     VSFPDGEGHILHELPAPQEKHMFFASDVVTWARTMSSAHDNTNVNDNSFFMKTSE"
     repeat_region   11702..13972
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11768..11799
                     /rpt_unit_seq="gtttcaatccacgcgcccgcgcggggcgcgac"
     gene            complement(14141..14431)
                     /gene="cas2"
                     /locus_tag="PKJ16_07780"
     CDS             complement(14141..14431)
                     /gene="cas2"
                     /locus_tag="PKJ16_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HNV46924.1"
                     /translation="MMVLVSYDVSTLDGAGRRRLRLIAKECVNNGVRVQKSVFECNVD
                     PARWETFKANLLGICNTKEDSLRFYFLGANWKRRVEHHGIHAPVETDEPMIV"
     gene            complement(14443..>15078)
                     /gene="cas1"
                     /locus_tag="PKJ16_07785"
     CDS             complement(14443..>15078)
                     /gene="cas1"
                     /locus_tag="PKJ16_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HNV46925.1"
                     /translation="ATDCENVENAVRKMAAVLERVKTATDSDELRGYEGECASLYFGC
                     FNSLITAQKDVFCFNGRSKRPPLDNVNALLSFMYAILANDIRSALETTGLDPQVGFFH
                     RLRPGRPSLALDIMEEFRMYMADRIVLNLINLKQVAAGDFEKRETGEIRLSDRARKEV
                     ITAYQKNKDEVIVHPLLDEKTTKGLLFHVQSMLLARHVRGQAADYPPFYMK"
BASE COUNT         2978 a         4889 c         4399 g         2812 t
ORIGIN      
        1 ggaggcagcc tggtgtattt caaaattgac atcgcgcgcg ttctcgaggt aggtgcggta
       61 cagctcgcgg tcatttgcgc tatcatctat gatcagaatc gcatgccgct cctgatcggc
      121 attcggcaac agaccgtaat cggggagcgg gaacccggtg tgcgtcatga ggacctcgta
      181 gggtatatgg tacaagtcgc cgagcagttt aagcttttcc agcgcgggac gcttcacgta
      241 gccattctcc atctggctga ggtaggcgtt cgatatgccg gtgctggctt ccacgtcacg
      301 aatggtcagg gaaagcgcct ctcttcctgc cttaaggagc actccgaatc tgttcatctg
      361 tgtaatccct cttaagatga cagtagccac tcccgcttga taaatcaagc aaatatggct
      421 gatttaacac ttatttatat ttttaaagct tggttgtgat taattcgaac atattacatg
      481 ggcagttact catatatgtt tataatccgc tattctcacg gaacgaatgg gattcgcaag
      541 agagttctat tctttgccgg acagaagaaa ttgggaaatt gtcgatgata tccgccgacc
      601 gcatcaccga cgtcccccgc tcgttcatcc gggagatcct caaggtcacg ctggaccgtt
      661 ccgtgatctc gttcgccggg gggctcccca accgcgatct cttcccgatc gcagagctca
      721 aggccgccgc catgcgcgtc ttcgacgagg agggcgcggt cgccctgcat gatcgctcgc
      781 acagaataaa caggcaggcg gccttatccg aaccgcaatg gtatcacagc gacgggccta
      841 gaggttttca caacaaaagt cgcacggcgg gagcaacaaa tgtttactgc cttacagcgt
      901 attgtctatc ggattgataa tgcgggaaac aactgccatg aaatcattct cgcttcttgt
      961 cacgagttca aggttcttgc tcaaggcggt ggctgcaatt atgcagtcgg gtatttttaa
     1021 ttttcttgcc tttcttatcc ttatgcattc ttcgacaata gaccgatcag cattgataat
     1081 ctctagagaa tcaagaaaac tgcggacttt ttccgacttt tcatcattca ttggacaaga
     1141 aagcacttca atataggtca cgaaagaaac ggatacatca cccagattat tcaataaaaa
     1201 accggttgcc tgttcccatc cattcagatg ataaataaga atattcgtgt caacgagata
     1261 tttctaaatg gattattccc attcgtttct aatgaatttt tgatactcca caccgtctat
     1321 gtttttctct ttcaggatgc ctgccgtatt gaaaagcttt ttcttttttt catcaatagt
     1381 atcgacaatt actagatcga ctgatatttt gctttgatcc gacggaatgc cctcgacatc
     1441 tggaataaag aatccgccgt ctttttttat tgcctgtaga atcattgaaa tacaccattt
     1501 cccctgtcat tccaccagaa acggttacgc tgaattttaa gtgcaaatag atatcccggt
     1561 tcctgattat tctatctaat caatgtaata agaaaaatca ttgtcaacaa taaaaaagcg
     1621 ggaaagcatc cctcgagctg ccggtaaagg tcgagaaggt tttgatgaac aatggataat
     1681 gccggggagg atgcgtacgg ggacaaaagc gcgattgaga aaaccggaac cgtgtaataa
     1741 aaataatatt gacgacttcc gcccgccgca tcatgctgca ctcatctttc aaggaaacgg
     1801 ttccggaaaa accataatgt ctctcgtccg cgcacatcgg cagctctcca tctccattgc
     1861 gattcccgca gtggtcgtcg ccgtatgcgc gctccccggt ttcgcgccga cgggtcttgg
     1921 atgagagagt acgatatctc gaacaccaca agcccctgtc ggcacaaccg gcaggggctt
     1981 ttttattggc cacagccacc tcatcccccg gccgccgagc gctttttgga cctcctgtcc
     2041 gcactcggca ctgcaacatc ctgttgggag ccttctccca caggagaagg ggggaatagt
     2101 agatttacta tttcaaagcc ctctcctatg ggagagggcg cgccggaggc ggggtgaggt
     2161 catacgagga atattttcct caacagaaag gaggccccca tggaagccgc attcgccgac
     2221 cgcatcaccg acgtcccccg ctcgttcatc cgcgagatcc tcaaggtcac gctggaccgg
     2281 tccgtcatct cgttcgccgg gggactcccc aaccgcgatc tcttcccgat cgcagagctc
     2341 aaggccgccg ccatgcgcgt cttcgacgag gagggcgcgg acgccctgca gtacggcccc
     2401 tcggagggtc atgcgccgct gcgcgactac atctcacgac gctatcggga acggctgggc
     2461 ctggagatcc cgccggagcg catcctcatc accagcggct cgcagcaggg gctcgatctc
     2521 ctcgccaaga ccttccttaa cgagggcgac gacatcctca tcgaggagcc gggctacctg
     2581 ggcgcgatcc aggccttctc cgtgtaccgc gcgagatttg tgccggtgcc gctgaccgaa
     2641 agcggcatgg acatcgaacg gctcacgggg gcgatggcgt cgtcccggcc caagctcgcg
     2701 tatatggtgc ccaacttcca gaacccatcg ggcatcagct actccgcaga gaaccggcgg
     2761 gccgtggcgg aggccatcgg cggcacgcgc accatcatca tcgaggatga tccctacggc
     2821 gagctgcgct tcgacgggac ccccgccccg tcattcggcg cgctcgcgcc cgagaacacg
     2881 atcctgctcg gatccttctc gaagaccgtg gtcccgggct tccgcctcgg atggatcgcc
     2941 gcgccgccgg ccgtgatgga ccggctcctc gtggccaagc aggcctccga cctgcacacg
     3001 aattcgttct cgcagcgggt gctcgcgcgc tatctcctcg acgcggaccc cgacgcgcac
     3061 atcgcgcgca tcatcgccgt gtaccgcgag cagcgcgacg cgatgctccg cgcgatcgac
     3121 gggcatttcc cgggctcggt ccggcgcacc cggccccagg gcgggatgtt cctgtgggcc
     3181 acgctgccgg agggcgcctc gtcgctcgcg ctcttcgaga aggccgcggc gcggaaggtg
     3241 gtgttcgtgc cgggcacacc gttttacatc ggccgcgacg acgcgcccac gatgcggctc
     3301 aacttctcga gcgtggatgc ggcgacgatc gcggagggaa tccgtcggct gggggatgcg
     3361 atgcgggagg gataagggca gacgcattag cagaaaaaca tcaggagcgc cgcgtctgct
     3421 cctcgaaagc gacgctcacc tccgcgccgg acagggacgc cgcgatcccc tgctcgatct
     3481 cgcggcggat cgccgtgatc tcgcgcaggg ggcggtggcc cgcagcggcc aatccgatca
     3541 cgacaaagag cttccgtccg gcctttctga tcctgatatg ccgcgtcgca ccgatacgat
     3601 ggcgcgcgag gtagcccgat agaagttcgt caattcgcag acgcatatcc tcgccggggc
     3661 tcgcatcgac gatatcgaga aagccgtcgc gcaggatgag cagcggcttg cgcatgagga
     3721 cacacgacag cgcaatgcac atgacgggat cgacataatt caggaaccgt tcgtccacga
     3781 cgcccttcag ggaggcgatc gccacaaagg cgacgaacac gcacaggtcc aaaagtccat
     3841 cgatgatcca gaggacgcca tttgcggcga ggatgtccga attcagccgc ttccccatga
     3901 tccgcatgaa cgcaccgaat ccggtgcaca cgaacatccc gataccggca taccatatcg
     3961 ccagggacag ccgcgcgacc gggtcgtgat ggacgatatc ctggatgctc ccgatgatgg
     4021 cggccacgca cagcgcggtg atcaggacgc cgttgagcgt cgtctgcacc ggttcgtatt
     4081 tcgcatagcc gaagtgatac cgttcgttcg gcggttcgcc cagcctgcgc acagtgtaga
     4141 tggcgaacag cgagatgatg acgtccgcga cgcaatagag cgcatccagc agcaccgtca
     4201 ttgaatcgca caggacggcg atgggaagac tcacgcaggc atagacgacc gaaagcgcga
     4261 gcgacatgcg catgagattc agttcattga atcgcatctt tttctccgct tttcgattca
     4321 cggccattcc cgcattgttc gatctgcgcc tggtgcgcct acaggcgtta cgatcgccct
     4381 ccaggacgga acagccgatc gaggggcacg ccccccgccg cacctccgcc agggcatttc
     4441 gatgttgcca taatcatacc ccgcccgatg ccgcgcgcaa cacattttcc cgacccttcg
     4501 cgccgcgacg gcaaaaatgt gattgatgac cccggcgcct ccgcgctatc atgcctgcgc
     4561 ataccacccc cgtccaggag cgcttcgcat gatacggaaa aagatcaccc ttatcgcact
     4621 cgtcatcctc gcactcggcc tcgccggata cggcggcctg cggctcgcct tccgctgttc
     4681 ggtcgatccg cccacgggcg ccatcaccct ccccgggatc acgggaacgg tcgtcatccg
     4741 tcgggacgcg ctcggcattc cgctcatcga agccgcgaac gagcccgacc tgtatttcgc
     4801 gacgggctat gcgcaggcct ccgaccgcct gtggcagatg gtgttcatgc ggatggtgat
     4861 gcagggacgc ctcgccgaga tcatcggcgc gggggggctc gatttcgaca tcgccatgcg
     4921 cagcctgcac gcgcgccgct acgtcgacga ggccatggcg cgcctgcggc cgggcacgcg
     4981 cgcgctcctg gaggcctact cgcgcggcgt gaacgcctac atcgagacgc accgcatcct
     5041 gccgcccgag ttcatcctca cgcgccaccg ccccgagccg tggaaaccgg aagactgctt
     5101 ctacgtcttc ggcctgctca atatgagcgt ctcgggaaac ctcatcgagg agctcggctt
     5161 tctcctggcg gcgaaccgcc tgggctacgg gaaggcggcg gtcctgttcc cggtctatcc
     5221 cgacgaaccg ctccccctgg ccgaggcgcg caagctcgcc gggatcgaga cgcgcgatct
     5281 cgccgcgccc ctcgatcgcc tctcggcctc cctgcgctcc ctgacgaacg tcatgcccat
     5341 gaacaaaccg gcgtccaata attgggccgt ggccggatcg cgcacgaaaa gcggccgtcc
     5401 catcgtgacc aacgacacgc acctcgatct catgatcccc aactcgtgga tgatcatgca
     5461 cttgaagtgc cccacgctgg acgtcgccgg ggtgatggcg ccgggtctcc cgatcgtctg
     5521 ctgcggcttt aacggatcgg tcgcctgggg cgcgaccatg gtgatggccg acagccagga
     5581 cctcttcctt gaaaagctga agaccgacgg gggatcgcgc tcctatctgt acaagggcgc
     5641 ctggcggccg gtgagcatgc ggaccgagac cttccgcgtg gccggcggcg agacggtgca
     5701 gcgcgaattc gcctcgacga tacacgggcc tctcttcaac gaagccctgg agcgaatgcc
     5761 ctttccgccc gagatgccgg tgcaaacctt gccgatgaaa tcgtcctacg gcctcgcgct
     5821 ctcctgggcg ctcgagggcg gaagcgactc attggagggg ttcctcgcgc tcggcagggc
     5881 gcgcacgatg gccgaggcac gggcggcgct cctgcgggtg aagagcatct atttgaacat
     5941 cgtgtacggc gatcggaccg ggatcgcatg gcaggtaacg ggcctgttcc cgacccgccg
     6001 cgcggggacc ggtcagttcc cctcacccgg ctgggacggc gcgcacgact ggacgggctt
     6061 cgtgcccatg gagcgcaatc cgagctcgct caacccggcc gagggataca tcggcacggc
     6121 gaacaaccgg accgtttccg cgaaccaccc cttcgtgatg agcacgagct ggttcgggcc
     6181 cgaccgcgcc gaccgcatcg cgcaggtcct ctcgggaacg cgcacggcga ccgcggagag
     6241 catgcacgcc ctgcacttcg accgctattc ccggttggcc gcgaagatgc agcgcctgct
     6301 ctccgacggg ccgctcgccg aacgcgtgcg cgcctccatc gcgcgcctga gcgacgaaga
     6361 aaaacggcgc gcggcgacgc aggccctcgc gatgctcgct ccgaccgcct tcgactgcgt
     6421 gatgcgaccg gaatcggcga acgccgcgct ctacggcgcc tttgtgcaca cattcacgcg
     6481 cgcgaccttc ctcgacgagc tcggccccga tgacagcgtc gtctgggagt cctttgtcca
     6541 tacgaacatg atttcctact cggccatcga ggaccacctg cagggccgcg aggactcccc
     6601 gttctggaac gacgtgggca gcggcggacg cgaggacaag gccgacatcc tcgccgagtc
     6661 gctggcgcgc gcgtacgccc tctgcgggga gcggatgggc ggcgacccga agaaatggca
     6721 gtggggcgcg ctgcacacct atcactggcg ccatgaattc gccaagaagc tgcccattct
     6781 gcacggctac ttcaaccgcg ggccgcatcc cgccggcggc gacggccaga ccgtcaatgt
     6841 cgcggcgtgg gccccgggaa agaacttcga cacctggctc atccccgcca tgcggctcgt
     6901 ggtcgatttt ggcctggccg acccggcgtt cctggtgacc gtgccgggcc agtcgggcaa
     6961 tccctcgagc gtgcactacg gggacatgat cgagcactac ctgacgggta agaaccatcc
     7021 cctgcccttc gccacgaagg cgatcgacgc gcagtacgcc gaccggctgg agctgaagcc
     7081 gcggtagggt ttccagcgaa aacgacgacg gaccacagga ggcgcaccga atgaacgcaa
     7141 agaaggaaat ccgccgcggc ctgcccatcg gaatcatcat cgtccttctg ctcggctgca
     7201 cgcccgccgc gcagaaggtc gcccccggcg actatcgcat aacgcgtccc ggcgcgacgt
     7261 ccgtcgtcgt cgtccattcg cgctccggca acaccgcgct ggtcgcgaag cttctcgccg
     7321 atgcggccgg ggcgacctat gtccgcctca aggcgcccga gggaacgggc gactccctga
     7381 tctcgtcgcc gaaccggaac gccgaggtcg ccatcgagcc gcgcacggtg gacctctcgg
     7441 cataccgcac ggtcttcctc ggcacgccca tctggttctg gcacccggcg gcccccatct
     7501 gctccttcat tcgcgcgaac gacctcaccg gcaagcgggt ggtcctcttc tacacctacc
     7561 agggcggcct ggccaagacc gcgatcgacg aatggaaggc gctcgtcgcc aagcgcggcg
     7621 ggacggtggt cgaggtgatc ggcatcaacc ggaagatgca cgcgaccgat gaggcgcttg
     7681 cggccgaggt gacgtcgatc atcgcggggc gcctgaagtc cctgctgccg tcgaagtaac
     7741 gccccgccat catcacgtct ccggcgctcg cgtcgcggat caccccgacg gcgtgcgggc
     7801 gtcatggacg ggatttttgt tgttttttcc tgcgacgctt cgtacacttc gacatatcac
     7861 acagatacac aacggagcgt ccaccatggc ggatacgata acacgggacc aacggcccga
     7921 gcggggcggc tcgctggccg acagcatcaa gcggcacatc gtgctcatgc tgggaaacga
     7981 ctttcatccc ccgcgcaagg acacctacta caacgggctc gcctactgcg tgcgcgaccg
     8041 gctcatcgag cgatggctct cggcgcagcg cctgtactac gacacccact ccaagcgcgt
     8101 gtactatctc tcgatggaat tcctgccggg tcggttcatg aagaactacc tcatcaacct
     8161 cgagatggag gacgaggtga ccgcggcgct cgaggagacc ggcttcacga tcgacgagct
     8221 cgcggaggag gagccggacc ccggactggg caacggcggc ctggggcgcc tcgcatcgtg
     8281 ctacctggac tcgatgtcgc gcctgggcat ccccggctac ggctgcggca tccgctacga
     8341 ctacggcatc ttccaccagg gcatcgtgga cggctaccag gtggaggagt gcgacaactg
     8401 gctgcagtac ggcaacccgt gggagatcgc gcggcggaac ttcatgtaca ccgtgaagtt
     8461 cggcggccgc tcggagccct ataccgacga caccggccgc gtccgctacc gctgggtggg
     8521 caccgacaac gtgaacgcga tggcctgcga catcctcatc cccggctacg gcaactacac
     8581 cgtgaacaac atgcgcctgt gggccgccgt gtccagccgc gacttcaacc tggacttctt
     8641 caaccacggg gactacatcc gcgccatgca ggacaaggtc ctcacggaga acatctccaa
     8701 ggtgctctac ccgagcgacg aggtggagca gggacgggag ctgcgcctca agcagcagta
     8761 cttcttcgtc gcggccacct tccaggacat catccgccgg ttccgcaagg agcacgcgga
     8821 ccatcgcacc ctgcccgacc gcgtggcggt gcagctcaac gacacgcacc cctcgatcgc
     8881 catccccgag ctcatgcgga tcctcctgga cgaggaggcg ctggactggg acgaggcatg
     8941 ggacatctgc gtgcgcacct tcgcctacac caaccacacg gtgctccccg aggcgctgga
     9001 gacctggccg gtcgcgctca tgggcaggct cctgccgcgg catctcgaga tcatctacga
     9061 gatcaatcgc cgattcctcg aggaggtgcg ccggcggcat cccgggaacg acgcgctggc
     9121 cgcgaagctc tcgctcgtcc aggagcaccc ggagaagcgc gtccgcatgg cgcacctggc
     9181 gatcgtgggt agccactcgg tgaacggcgt ggccgagctc cactcgcgca tcctcacgac
     9241 gagcctcttc cgggagttcc acgagttctt ccccggccgg ttcacgaacg tcaccaacgg
     9301 catcacccag cgccgctggc tcctgcaggc caatcccgcg ctcgcgcggc tcatcaccgg
     9361 ggcgatcggg ccgggatgga tcaacgagct ctcggagctc aaaaaactcg ttcccctcgc
     9421 ggaggacgcc tcgttccggg ccgcctggcg cgaggtgaag cgggccaaca aggagcggct
     9481 ggccgcctac atcgcgaagg ccaccgggat caccgtgaat ccggattcgc tcttcgacat
     9541 ccagatcaag cgcatccacg agtacaagcg ccagctcctg aacgtgctgc acgcgatcac
     9601 gctctacaac cgcatcagga cgaatccgaa cgatcacttc gccccgcgca ccgtcatcat
     9661 cggcggcaag gcggcgccgg cctacgcgat ggcgaagctc atcatcaagc tcgtgaacgc
     9721 cgtcgcggag acggtcaacc gcgacccggc cgtgggcgac cgcctgcgcg tcgccttcct
     9781 ccccaactac aacgtgtctc tggccgagcg cgtgatgccg gccgcggacc tctccgagca
     9841 gatctcgacg gcgggcatgg aggcctccgg cacgggcaac atgaagttct cgctcaacgg
     9901 cgcgctcacc atcggcacgc tggacggggc gaacatcgag atcatggagg aggtggggcg
     9961 cgagaacatc ttcatcttcg gcctgcgcat cgaggaggtg gagcgcctgc gcacgaccgg
    10021 ctatgatccg cgcgaacact accgcagcga cgccgagctg cggcaaacgc tcgacctgat
    10081 cgcctccggg tacttctcgc cggagcggcc cgatctcttc gcgcctctcg tgcgcgcgct
    10141 tctcgacggc ggcgaccgct acttcctgtg cgccgacttc cgctcgtacg ttgacgagca
    10201 ggacaaggtg cgcgcgctct accgcgaccc ggaggcgtgg tcgcgcacgt cgatcatcaa
    10261 cgtggcgaac atgggcaagt tctcgagcga ccgcgcggtg cgcgaatacg cggagcggat
    10321 ctgggggatc gggatcacgt gaccccctcc gtccttcgga catcaggaga aaccatggac
    10381 gaatcgacac cgatagccaa cctcaagaaa atggccctcg agttccgcga tgcgcgcaac
    10441 tggcggcagt tccacgacct gcgcaatctc gcgaccgggc tttccatcga gagcgccgag
    10501 ctgcaggaga tattcctgtg gaagaacgac gccgatatcg cgcgcatcgc gcaggacgag
    10561 tcggtgatgc ggcggctgcg cgaggagatg gccgacatct tcatctacct tttgtacctg
    10621 agcgaggcga cgggcatcga cctcgcggac gcggtcgcgg acaagatcga actaaacggg
    10681 aagaagtatc ccgtggagaa gagctacaac tccagcaaga agtatacgga gctgtgagga
    10741 aaaaactatt cggaggtttt catgaaaaag ctgttatcgt tcacattcgt attgtcgtgc
    10801 gcgctgctca tggttctcgc ccacgtcacc acgtccgacg cgaagaacat atgcttttcc
    10861 tgcggcgcag gcagttcgtg caagatgtgt ccctcgccgt ccgggaagga caccgcaaaa
    10921 gacagaaagc tctgcaggga gcggggctgc aaggtcgccg gaacaacgtc gtgctcaacc
    10981 ccttccggcg tgaaaatttg ccgatagcgt cgggcatgcg cgcatcgtct cgcgcgcggc
    11041 gacattgagg agcccatacg ccccggacga acgtgcccgt ccggggtctt ttacctcacc
    11101 cgccctccgg gccgccctct cccattggag agggctcttc tggacaggac ctatgacaag
    11161 gagcccctct ttgcgcgcag agaggggcgc ggcaggccgg gggtgagtcg ggttcgttcc
    11221 gtgccctcat gggattttct tgttgatcgc tatgcgggct ctttgcgtga gatcgctctt
    11281 ggcgtgtacc agcgtttgtg ctgtgagggg aagaggccca cgcgggcctc ttcttcgtcg
    11341 atcgccattc gggcctcctg cccgcgatcg acattagcgc atccgtgcgc gtcacctcac
    11401 gctccccttc tccgcgcccg ctcaacgccg cggggggctt gataggcttc gttttaatgg
    11461 gctggtgggt tcggggatgg cgtgtctggt ttatgccccg ggctctgctg cgcgtagacc
    11521 gccgcccggg cgtcttttac ctcacccgcc ctccgggccg ccctctccca ttggagaggg
    11581 ctcttctgga caggacctat gacaaggagc ccctctttgc gcgcagagag gggcgcggca
    11641 atgccggggg tgagtcgggt tcaatccacg cgccggtgta gtcgtatttc cggctcagat
    11701 agtttcaatc cacgcgcctg cgcggggcgc gacacggatc gcacgccggg tccggactgg
    11761 ttatatggtt tcaatccacg cgcccgcgcg gggcgcgacg ttgatatggc gcatccacag
    11821 ccgcaccggc tcgtttcaat ccacgcgccc gcgcggggcg cgaccgcgtc aaccggcgtt
    11881 acaatcgagc ataacgacgt ttcaatccac gcgcccgcgc ggggcgcgac gggacacaag
    11941 cagcacggac ccgcatcatc gccagtttca atccacgcgc ccgcgcgggg cgcgacttag
    12001 atcattgaaa acatgccggg ccgcgcgagc gtttcaatcc acgcgcccgc gcggggcgcg
    12061 accttcgcca tccgctccac cggggatgga taggcgtttc aatccacgcg cccgcgcggg
    12121 gcgcgacgac aggcggcggg gaaataacac gatacggggt gtttcaatcc acgcgcccgc
    12181 gcggggcgcg acctgtatac atttacgttg cgtcaattgt ccgaacgttt caatccacgc
    12241 gcccgcgcgg ggcgcgacct attataaaca aacctattaa aaaaaaatgc gagtttcaat
    12301 ccacgcgccc gcgcggggcg cgacacatcc ttccgggcgg tatggtcatg ccggtcaagt
    12361 ttcaatccac gcgcccgcgc ggggcgcgac ctggcgggat gtacagaagg agttgccaga
    12421 agagtttcaa tccacgcgcc cgcgcggggc gcgacgttcg atcagtttgg tgagcaggga
    12481 aacgatgatg tttcaatcca cgcgcccgcg cggggcgcga cagatgaaaa tcaatgtata
    12541 ttgacccaat tcgatgtttc aatccacgcg cccgcgcggg gcgcgacatg taatggccgc
    12601 cgtgagattc gatattctcc ttgtttcaat ccacgcgccc gcgcggggcg cgaccgtgag
    12661 aacacgatgg cgattcagat gcaacggcag tttcaatcca cgcgcccgcg cggggcgcga
    12721 ccgtgatcgt atgccagttt aatctcagct ttcatcgttt caatccacgc gcccgcgcgg
    12781 ggcgcgacac gcctcacctc cgtcatgcgg gacatgctgt ggtttcaatc cacgcgcccg
    12841 cgcggggcgc gaccagcggg agtgggccga gtggatgcaa gaggccgttt caatccacgc
    12901 gcccgcgcgg ggcgcgacag ccacaggggc cgccgcctac gccggagatg ccgtttcaat
    12961 ccacgcgccc gcgcggggcg cgacgcagtg cgacatcatc accggctacg agaagtcgtt
    13021 tcaatccacg cgcccgcgcg gggcgcgaca taataataat cttaaaaaaa atgcgaaaaa
    13081 aatgtttcaa tccacgcgcc cgcgcggggc gcgacaatcc tgatttttct aaatatcgcc
    13141 ctatttgcgt ttcaatccac gcgcccgcgc ggggcgcgac gcggattgtg cgattattga
    13201 tacagttaca tcggagtttc aatccacgcg cccgcgcggg gcgcgacatc aaaatttttc
    13261 gctgactatc gcgattattt gtttcaatcc acgcgcccgc gcggggcgcg acccgcacgt
    13321 atgccgattg ttgcgcccga cgcgccgttt caatccacgc gcccgcgcgg ggcgcgactc
    13381 ctgcctcgct attattaaat caatattctc atgtttcaat ccacgcgccc gcgcggggcg
    13441 cgacataacc ggcatcatgc gccccctgtc ctccggtgtt tcaatccacg cgcccgcgcg
    13501 gggcgcgacg acattttaca tacccctaat tattagattt atagtttcaa tccacgcgcc
    13561 cgcgcggggc gcgacgtaaa tttaaacgaa tacgagagac atttctcgaa agtttcaatc
    13621 cacgcgcccg cgcggggcgc gactttttaa gataatataa taatagaaga aagcgcagtt
    13681 tcaatccacg cgcccgcgcg gggcgcgacc gcccgacgcg cccgcgcccg acgcgcccgc
    13741 gccgtttcaa tccacgcgcc cgcgcggggc gcgactgcat cagattttca gtaatcaggc
    13801 cgccgtgtag tttcaatcca cgcgcccgcg cggggcgcga ctttatcccc ggcgcgcgcg
    13861 gtgagcagtt tacgtttcaa tccacgcgcc cgcgcggggc gcgacccgac tgtaagaccg
    13921 atcgggcgct cacaagccgc gtttcaatcc acgcgcccgc gcggggcgcg actgccctac
    13981 gagtaaacac tgatgccgtc agggtttacg gtatacaaaa cgctaaacta taagttcacc
    14041 cagtgaaaat ataccttttt gccatactcg aatgatggca tataaggaag aagtttattc
    14101 cgctaacacc ctgaagaata gccatcactt atggttagcg ctacacgatc atcggttcat
    14161 cggtttcaac cggcgcatga attccatggt gttcgaccct tcgtttccaa ttcgccccaa
    14221 gaaagtaaaa acgcaggctg tcctctttcg tattgcagat gccgagaagg ttcgccttga
    14281 acgtctccca gcgcgctgga tcgacattgc attcgaatac cgacttctgg acccgcaccc
    14341 cgttgttcac gcattccttc gcaatgagtc gaagccttct gcgaccggcc ccgtcgagcg
    14401 tagaaacgtc gtacgacaca agcaccatca tggcaggttc cgctacttca tgtaaaacgg
    14461 cgggtaatcc gccgcctggc ccctgacatg ccgggccagg agcatcgatt gaacatgaaa
    14521 cagaagaccc ttggtggtct tttcatcgag caggggatgc acgatcacct cgtccttgtt
    14581 tttctgataa gccgttatta cctctttgcg cgccctgtcg gacaggcgaa tctctccggt
    14641 ttcacgtttc tcgaaatcgc cagccgcaac ctgtttcagg ttaataaggt tcaacactat
    14701 ccgatcggcc atgtacattc gaaattcctc catgatatca agcgccaggc tgggccgtcc
    14761 cggccgcaac cggtggaaaa aacccacctg cggatcaagc ccggtcgttt cgagggccga
    14821 ccgtatatcg ttggccagga tggcgtacat gaaagacagg agcgcattta cattgtcgag
    14881 tggcggacgt ttcgagcgcc cgttaaaaca aaagacgtcc ttctgtgcgg taatgagcga
    14941 gttaaaacaa ccgaaataga gcgatgcgca ttcgccctca tacccacgca attcgtcgga
    15001 atccgtcgcc gttttaacgc gctcaagcac cgcggccatc ttccggaccg cattctccac
    15061 gttctcgcag tccgtagc
//
LOCUS       DAORHF010000070        15044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3023_length_15044_cov_4.887985, whole genome shotgun sequence.
ACCESSION   DAORHF010000070 DAORHF010000000
VERSION     DAORHF010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 15044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15044
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3023_length_15044_cov_4.887985"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="PKJ16_07790"
     CDS             <1..1211
                     /locus_tag="PKJ16_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNV46926.1"
                     /translation="GRWVFVALGFVMNICMGSIYAYSIFKPFIEREFDVSPLASAIPF
                     MLFSAFFAFLMFFGGKFMEIMGPRYLSIVGGVIMAIGWMLASYASNAFMLAVSYGVIG
                     GAGVGLAYGCPIAIAAHWFPDRTELAANIMLAGFGGSAVITGTAAMLLIDRAGVTGVF
                     MNFGITFLVVLFAVSLPLRFPAPGWRPAGWAPADISGNDVDFDSHEMIRTASFWGLSL
                     CVIIGCTAGLMAICIAVPAGMELAGLDFGVAAALVGLFSAFNALGRILFGALSDRTAP
                     RRGAMINFAIVLGACALMVAPIGADTARYIIAFCALWLTLGGWLAIAPAATASFFGIR
                     HYAKNYGVIFFAYGIGAVLGGAIGGASRLLFGSYRFAFHPVLALAALGIVLAALLITP
                     PRTRRIDGPR"
     gene            complement(1240..2535)
                     /locus_tag="PKJ16_07795"
     CDS             complement(1240..2535)
                     /locus_tag="PKJ16_07795"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HNV46927.1"
                     /translation="MNMHEAVQTVKKASIRLAAAGAEVKNAALMAIADALLARENDIV
                     KANLADLERSEKEGLAAPLMKRLRFDSAKIRDVVAGIESLMALPDPVGRTQAATELDE
                     GLALYRVSCPIGVIGIIFESRPDALVQISTLCLKSGNGVLLKGGSEARETNRILADII
                     AGATINTGVPDGWLALLETRDDVNAMLAMDEYIDLIVPRGSNEFVRYIMDHSNIPVLG
                     HADGICHAYVDAGADIDMAVRVVVDSKVQYVAVCNALETLLVHREIAPAFLPAAKEAL
                     ERHHVELRGCERTRAIVDCAPATEEDWKTEYLDYVLSIKVVDGLEEAIDHINAYGSGH
                     TDVIITANERAAMEFMDLVDSGDVFWNCSTRFSDGFRYGLGAEVGISTNKIHARGPVG
                     LEGLVIYKYKLVGSGQTVADYAEGRKKFTHRRLDRGFPL"
     gene            complement(2591..3205)
                     /locus_tag="PKJ16_07800"
     CDS             complement(2591..3205)
                     /locus_tag="PKJ16_07800"
                     /inference="COORDINATES: protein motif:HMM:NF022586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2889 domain-containing protein"
                     /protein_id="HNV46928.1"
                     /translation="MASLKECARDLVHRRDISIATYAVDDHTIMAEGALVDDRTNEYH
                     LLSGEARPAGIIHHMVIRVLAEGPALIIREIEVEMPGAPRAECAETRDCLAGARGMAI
                     GPGFTKQVKALAGGPRGCAHLVTLLLAIAPAIIQGYWAHRARRRVTADTAAPRKKMDG
                     MLRNTCHVWREDGPTYAKLMRHLDGETSSPQPPSPAGEGGALKR"
     gene            3327..4478
                     /locus_tag="PKJ16_07805"
     CDS             3327..4478
                     /locus_tag="PKJ16_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46929.1"
                     /translation="MDGHDDNAPWGPVGPDAPDTDEALERAYTERKARYYRHVLRKED
                     GALLRRILKIPDSWSDYERAHGAGASLSDHQKKRLSDIKKSKGDELAFESRYILGLIT
                     IENALLACLQYHESCAALADDALESAREILAGAAAGPFNFKHLLLSLAKRSISDLYYL
                     MASYQKFYHKKCFEFDADFTAVVERIAGTIRAAGTLDERLRGFSKYREIIAPFFTRSS
                     TYAGLRLDEISLKRQYESGGIGIKPEHLEIVVPALDAYRTELRRNHDFIAYYYNRTDG
                     KRYRYDFIRTALLQKNAAGGISEETLESFIAVHDSFCAIEARFLEKGLADFGPPALPY
                     GAATELAFQCCKVLEYLYLRGGHYEKLQDMRSRWLQHTEQDLHRLRQLA"
     gene            4572..4934
                     /locus_tag="PKJ16_07810"
     CDS             4572..4934
                     /locus_tag="PKJ16_07810"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HNV46930.1"
                     /translation="MIKGFNRQEKKAIIAILKFIIHADGVIMDNEIAKFNELAERKGF
                     EDFADVFNEVDREVRTLDDLRGVIAAVTSETHRNDILKYAVEIALADISIKPEEAQIL
                     KLVGKEWKINITALMKGK"
     gene            4954..7071
                     /locus_tag="PKJ16_07815"
     CDS             4954..7071
                     /locus_tag="PKJ16_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV46931.1"
                     /translation="MKVRTLILALLAAAALASGCAPHDAVTVDTGWRITRADAPEHAA
                     PGFDDAAWDAIRLPAVLSNEKRRQHFWLRARVTVPAAWKGKELAVRLGKIWDTDRTYV
                     NGRLVGATGSEYPDFNSAWFIDRTYFIPPDAVNYGGENVIAVRMFTNQNAMFNGTPLI
                     APAEPLRAYSFRKRFVAEYVPMGMGILTAFIGLFALTMYLFDRRSRLPLYLALTSFLW
                     TIMSTHFFVPDFVIIDFNTHDKLYYALISIEISLIYLFLEDILSVRIKAIRIIVLVLA
                     GLSFLVSVSATAESPITGWRFDVIGGLGILSQVFWGVLIVRGLRKRTPESRIVFIFYI
                     LFMVCLLHDSLAISNLISYESFWINLAYPSLMIAFSIILSMQTAVMARKLQISSVEIE
                     EKNRSLSEMLAGVRESASELTEFSQTLRKTATDLQNDMDEQGTNLDQTAAVIEEVSAS
                     IESVADNAARQDNVVRSNQDLLLEYVDSLRKITEAAKDAAKLSYRSQGQTAESRAKLD
                     DITAGMNKIKQSSGAINEITEIINDIAEKTNLLSLNASIEAARAGQYGRGFAVVADEI
                     GKLADSSIQQAKTIQGIIAGTVADIERETELVARSSRSIDEIEKAVNDVNRGIDAILD
                     LCLAQEKLTEEIGHNMKRILTGSSKISASTQEEKTSIAEVSKSIEHLTGIMETVKESA
                     AQVMSELERLYKRIEILHALVKD"
     gene            complement(7176..7871)
                     /locus_tag="PKJ16_07820"
     CDS             complement(7176..7871)
                     /locus_tag="PKJ16_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006098624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV46932.1"
                     /translation="MDRYQGKYRIASTRLKQWDYGANGCYFITMCVHDRVRAFGCVEN
                     RIMRLNPFGAIAERYWHEIPHHFPFVLLDAFVVMPDHVHGIIIIDRSIWAGLDHADIA
                     GHDAIVVGHDAVVARHGAIDVETGHCPVSTSQNAHHRTRENNETNARHQTHVHNEPDT
                     HRDTHMETKRKRGTISSIIGSYKSVCAKMIHRMPGGERFRWQSRFYDCIIRDEESLRA
                     VRNYIANNPMRWG"
     gene            8531..9418
                     /locus_tag="PKJ16_07825"
     CDS             8531..9418
                     /locus_tag="PKJ16_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46933.1"
                     /translation="MKTPMYCSMNRFIRSASTRGCFATRRAFFVFMIAAILAGLTLPS
                     CNTSSCQWVTTGSRKGKKTTEYVCGDKLKRIQRTRQNNAAARNRSVEENNAFTSKLAA
                     ARPSADQQVFIQLVKAEQAEKARMKGQTTAAQEAIAKSIQAKYKNRPISFRQVCCVNV
                     ERKFNTYTAVFYVPDPNAGGGCTGRPVDENFNFYGVPFVVKRNLSAAAAGRYKIGTAY
                     SVSGRLVVNENSFSTDYEENQYLLLRGVRPYEAGNWSSKTYPEGGKRLGGPDHQRRTD
                     VRKSYYGEFFTFSQIITLD"
     gene            9584..12433
                     /locus_tag="PKJ16_07830"
     CDS             9584..12433
                     /locus_tag="PKJ16_07830"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV46934.1"
                     /translation="MQRLCAALVLCVLAWTACTGCGPLSSKEKRPVAVKGVIDLRNWD
                     FERDGAINLNGEWEFCWDRELAPNDFSRTAGKTDCGFITVPGPWKEQTYNGAPLPGEG
                     RATYRLKIINGPDAKSKALTARGIFSVYKLWVNGVLLDRRGDSGRAAKTREDYIYVHN
                     RHLSSFTLQAGVNEIVLQVFNRDHASGGINRQLRMENGSTAARNEFYRLNIIMVIVGL
                     TLFSAIYNVLFFLFRRIDPTSLYIGLASLVSAVNTYNVEHPILTSGLSPTGNPFIINF
                     VTVIAGMLLFMMIAWSLFPDDFSKSIIRFAQAASACFIVPLFFVGFKTAQSIMYLFFA
                     FTVMLFTYGLFGLCKAIINKRRDALLFFASLCPIYIAAANNILYYLQVIDTGNLVPHA
                     MVLFCISSTIVVSRRFARALGTVEEMSRELTEKNITLERLDRLKDQFLAATSHELRTP
                     LHGMIGLSNSLANGDTGPLPPKAVETASLIAASGARLAAMVNDLLDMAKIQEEGLSLA
                     PRPVDLHALSETVVQLSLPLAGDKPMEIANRITPDIPAAWADEDRVRQVLYNLLGNAI
                     KFTTEGTIELSARVMRVSNGDHEGDARAMIEVRVSDTGIGVPEEYRESIFEAYRQADA
                     GDTRSYGGTGLGLAIAKQVVEAHGGSIAMAPRTGGGSVFSFTLPVSTAPTRDDETAPT
                     MTDMPGDAPTPNETGPSHLPAGINKNDLGKRPVILVVDDDTVSLRVVQDFFESRDITV
                     KTASNGIRALDLIEKDDSLSLVLLDIMMPIMSGYDVCRRIRISRSPEELPVIMLTAKN
                     MMADIDAAFAAGANDYLVKPFQPSELLARVSTMLKLRKVSRGTAEGITVRDRIRAYSI
                     AFGDITHITAHSKNVVIHTTESDIAIPTGLTEILDRLPPDLFVRIHKSHVVNLRHVHS
                     VSHVVSGRYRVRLRDKDDTELPVGPAHLGPLRTRM"
     gene            12708..>15044
                     /locus_tag="PKJ16_07835"
     CDS             12708..>15044
                     /locus_tag="PKJ16_07835"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNV46935.1"
                     /translation="MGILRRGGASSLRASHTIIVIFICILAFSLPACNRDVYDNLFDP
                     VNQYTVTFDSQGGTAVSAQTVDYGTTISQPPDPVLTGFTFAGWFAEPGCTTPWLFGSG
                     KVEGDTTLYARWTINQYTVAFDPQGGSAIAPQTVSHNGTAADPGAPARTGYTFGGWFQ
                     EAGCVTQWVFAIDTVTENRTLYAQWTINTYTLAYTAGANGSLTGSTTQFVTHGANGTA
                     VTAVPNVGYHFTSWSDGSTDNPRTDTGVTGNITVTANFAINQYTLSYSAGANGSLSGA
                     SAQVVNHGSDGTAVTVVPNVGYHFTSWSDGSTDNPRTDTNVTGTITVSANFAINQYTL
                     TYTSGANGTIAGTTPQTVNHGASGSAVTATPNVGYHFTSWSDGVLTATRTDTNVTGAI
                     TVTASFAINTYTLTYNAGAGGSITGSSPQTVNHGSNGTAVTATPNVGYHFTSWSDGGL
                     IATRTDTNVTGDMTVIANFAINTYTLTYTAGANGSISGTSPQTVNHGSDGTAVTATPN
                     LGYHFVDWSDGSTTNPRTDTNVTGAIIVSANFAVGGTLAASTFSVDSGSPCWDVAVNP
                     GANTVYASCGNRLHLLNAATGATIGIFATYNGVGVAVNLNTGLVYVVNSSISAGVTFF
                     NSDGTYRFGTLAASNFPTGLFPHHPAVNPTTGILHVTNYNSNTVTYLDAATGGLIGNY
                     PTGANPWGVAVNSTTNIVYVTSDSSSSKRYLDASSGALLGDYAIGSNSCMVAVNPAAN
                     IVYFTNTTANTVTYLNATTGALIGHFTTGSNPHGVAVNP"
BASE COUNT         2919 a         4925 c         4386 g         2814 t
ORIGIN      
        1 ggggccgctg ggtcttcgtg gccctcggct tcgtcatgaa catttgcatg ggcagcatat
       61 acgcctacag catcttcaag ccgttcatcg agcgcgaatt cgacgtttcc ccgcttgcaa
      121 gcgccatccc cttcatgctg ttttcggcct tcttcgcctt cctcatgttc ttcgggggaa
      181 agttcatgga gatcatggga ccgcgctacc tgtccatcgt gggcggggtg atcatggcca
      241 tcgggtggat gctcgcctcg tacgcatcga atgcgttcat gctggccgtc tcctacggcg
      301 tgatcggggg cgccggtgtc gggctcgcct acggctgccc catcgccatc gcggcgcatt
      361 ggtttcccga cagaacggag ctggccgcga atatcatgct cgccgggttc ggcgggtcgg
      421 ccgtcataac gggaacggcg gcgatgctgc tcatcgaccg ggcgggggtg acgggcgtct
      481 tcatgaactt cggcatcacc tttctggtcg tccttttcgc ggtctcgctg ccgctccgct
      541 tccccgcacc cggatggcgc cccgccggtt gggcgcccgc ggacatttcc gggaacgacg
      601 tcgatttcga ttcgcacgag atgatacgga cggcctcatt ctggggactc tcgctgtgcg
      661 tcatcatcgg ctgcacggcg gggctgatgg cgatatgcat cgcggtcccg gcggggatgg
      721 agcttgcggg tttggatttc ggcgtcgcgg cggcgctggt ggggctcttc tcggcgttca
      781 atgcgctggg gaggatcctc ttcggcgcgc tctccgaccg caccgcgccg cgccgggggg
      841 ccatgatcaa cttcgcgatc gtgctgggcg catgcgcgct catggtcgcg ccgatcggtg
      901 ccgataccgc gcgctatatc atcgccttct gcgcgctctg gctcacgctg ggcggctggc
      961 tcgccatcgc gcccgcggcc acggcgtcct ttttcggcat ccggcactat gcgaagaact
     1021 acggcgtcat cttcttcgcc tacggcatcg gcgcggtgct gggcggcgct atcggcggcg
     1081 cgtcgcggct gttgttcggc tcgtaccggt tcgcgttcca tccggttctc gcgcttgccg
     1141 cgctggggat tgtcctggcg gcgctcctca tcaccccgcc ccgaacgcgg cgcattgacg
     1201 gcccgcggtg atctcgcagg gccaacggca cgaaccgtcc tagagcggaa agccccgatc
     1261 gagacgccgg tgcgtgaatt tcttccgccc ctcggcgtag tcggcaaccg tctgcccgct
     1321 gccgacgagc ttgtacttgt agatcaccag cccctcgagg cccaccggcc cgcgcgcatg
     1381 aatcttgttg gtgctgatcc ccacctcggc gcccagccca tagcggaagc cgtcgctgaa
     1441 gcgcgtggag cagttccaga aaacgtcgcc ggaatcaacg agatccatga actccatcgc
     1501 ggcgcgctcg ttcgcggtga tgatgacgtc cgtgtgcccg gatccgtagg cgttgatgtg
     1561 gtcgatcgcc tcttcgagcc cgtccaccac cttgatggac agaacgtaat cgaggtattc
     1621 ggtcttccag tcctcctcgg tcgccggggc gcagtcgacg atggcgcgcg tgcgctcgca
     1681 gccgcgcagc tccacgtgat gacgctccag cgcctccttc gcggccggga ggaacgcggg
     1741 cgcgatctcc cggtgcacca agagcgtctc cagggcattg cacacggcca cgtactgcac
     1801 cttggaatcg acgaccacgc gcaccgccat gtcgatatcc gcgccggcat ccacgtaggc
     1861 gtggcagatg ccgtcggcgt gtcccagcac cggaatgttc gagtggtcca tgatgtagcg
     1921 cacgaactcg tttgatccgc gcggaacgat aaggtcgatg tactcgtcca tcgcgagcat
     1981 cgcgttcacg tcgtcgcgcg tctccaggag cgcgagccag ccgtcgggca cgccggtgtt
     2041 gatcgtcgcg ccggcgatga tgtccgcgag gatacggttg gtctcgcgcg cctcgctccc
     2101 ccctttcagg agcacgccgt tgccgctctt gaggcacagg gtggaaatct gcaccagcgc
     2161 gtccggccgc gactcgaaga tgatgccgat gacgccgatg gggcagctca cgcggtagag
     2221 cgcgagcccc tcgtcgagct cggtcgcggc ctgcgtgcgg cccacgggat ccgggagcgc
     2281 catgaggctc tcgataccgg cgacgacgtc gcggatcttc gcggaatcga accggaggcg
     2341 cttcatgagc ggagcggcga ggccctcctt ctcgcttcgt tcgagatcgg cgaggttcgc
     2401 cttgacgatg tcgttctcgc gtgccaggag cgcgtcggcg atcgccatga gcgcggcgtt
     2461 cttcacctcc gcgccggcgg ccgcaaggcg gatcgaggct ttttttactg tttgtacggc
     2521 ttcgtgcatg ttcatggttt taacctcacc cccggcccct ctcccatagg agaggggaga
     2581 ttccaataca ttacctctta agcgctcccc cttctcctgc gggagaaggg ggctgggggg
     2641 atgaggtttc cccgtccaga tgccgcatca gcttcgcgta ggtggggccg tcctcgcgcc
     2701 atacatggca ggtgttcctg agcatgccgt ccattttctt ccggggggcc gcggtatcgg
     2761 ccgtcacccg gcggcgggcg cgatgggccc agtagccctg aatgatcgcc ggcgcgatcg
     2821 cgagcaggag cgtcaccaag tgcgcgcacc cgcgcggccc cccggccagc gccttcacct
     2881 gcttcgtgaa gccggggccg atggccatgc cgcgcgctcc ggccaggcag tcccgcgtct
     2941 cggcgcactc ggcgcggggc gcgccgggca tctccacctc gatctcccgg atgatcagcg
     3001 ccggcccctc cgccaggacg cggatcacca tgtgatggat gatccccgcc gggcgcgcct
     3061 cgcccgacag gaggtggtac tcgttggtgc ggtcgtccac cagcgcccct tccgccatga
     3121 tggtatgatc gtccacggcg taggtggcga tgctgatatc gcgccggtgc accaggtcgc
     3181 gcgcgcattc cttcaacgat gccatgccgc ccctcctctt cgcgcggcga ttgtcgcata
     3241 ccgcgtgttt tctgtcaagg ggggcgcgcg gatcaatatc tttcttgaca cggccgcgcg
     3301 cggggcgtcc actgcgaagc gatcgcatgg atggacacga cgacaacgcg ccctggggac
     3361 cggtcggtcc cgacgcgccc gataccgacg aggcgctcga gcgcgcctat accgagcgca
     3421 aggcgcgcta ctatcgccac gtgctgcgca aggaggacgg cgcgctcctg cggcgcatcc
     3481 tcaagatacc cgacagctgg agcgactacg agcgcgcgca cggcgcaggc gcctccctgt
     3541 cggaccacca gaagaagcgc ctgtccgaca tcaagaaatc caagggcgac gagctcgcct
     3601 tcgagagccg gtacatcctc ggcctcatca ccatcgaaaa cgcgcttctg gcctgcctcc
     3661 aataccacga gtcctgcgcg gccctggccg acgacgcgct cgagagcgcc cgggagatcc
     3721 tcgccggcgc cgccgccggg ccgttcaact tcaaacacct gctcctgagc ctcgccaagc
     3781 gctccatcag cgacctgtac tatctgatgg cctcgtacca gaagttctat cataaaaagt
     3841 gcttcgagtt cgacgccgat ttcaccgccg tggtggagcg cattgcgggc acgatccgcg
     3901 ccgccggcac gctcgacgag cggttgcgcg gcttctcgaa ataccgcgag atcatcgccc
     3961 cgttcttcac gcgctcgtcc acctacgcgg ggctgcgcct ggacgagatc tcgctcaagc
     4021 gccagtacga gtccggcggg atcggcatca agccggagca ccttgagatc gtggtccccg
     4081 cgctggacgc ctaccggacc gaactgcgac gcaaccacga cttcatcgcc tactactaca
     4141 accgaaccga cggaaagcgc taccggtacg atttcatccg caccgccctc ttacagaaaa
     4201 atgccgcggg cgggatctcc gaggagacgc tcgagagctt catcgcggtg cacgattcct
     4261 tttgcgccat cgaggcgcgc ttcctcgaaa agggactggc cgatttcggg ccgcccgccc
     4321 tcccctacgg cgccgcgacg gagctcgcct tccagtgctg caaggtgctc gaatacctgt
     4381 acctgcgcgg gggccactac gagaagctcc aggacatgcg gtcgcgatgg ctgcaacaca
     4441 cggagcagga cctccaccgg ctgcgccagc tcgcgtgatc ctcctcgcgc cgccccggga
     4501 accataaaaa gtattgcaaa tattcaccgc atgtgcacac tctccgtatt tccacggcac
     4561 gagggggcgc catgatcaag ggattcaacc gacaggaaaa gaaggcgatc atcgcgatcc
     4621 tgaagttcat cattcacgcc gacggcgtga tcatggacaa cgagatcgcg aaattcaacg
     4681 agctggccga gcgcaagggc ttcgaggact tcgccgacgt cttcaacgag gtggaccgcg
     4741 aggtccgcac gctggacgac ctccggggcg tcatcgccgc cgtgaccagc gagacgcacc
     4801 gcaacgacat cctgaaatac gccgtcgaga tcgccctggc cgacatctcc atcaagcccg
     4861 aagaggcgca gatcctgaag ctcgtcggga aggaatggaa gatcaacatc accgcgctca
     4921 tgaagggaaa gtgagcgccc gccggataac cggatgaaag tccgcacgct gatcctcgcg
     4981 ctcctggcgg ccgccgcgct cgcgtcgggc tgcgcgccgc acgacgccgt gaccgtggac
     5041 acgggctggc gcataacgcg cgccgacgcg ccggagcacg ccgcccccgg cttcgacgac
     5101 gccgcctggg acgccattcg cctgcccgcg gtcctgtcga atgaaaagcg gcgccagcac
     5161 ttctggctgc gcgcgcgggt gaccgtgccc gcggcgtgga agggaaaaga gctggcggtg
     5221 cgcctgggca agatatggga caccgaccgc acctatgtca acgggcggct cgtgggcgcg
     5281 acgggatcgg agtatccgga cttcaactcc gcctggttca tcgaccggac ctattttatc
     5341 cctccggacg ccgtgaacta cggcggcgag aacgtgatcg ccgtccgcat gttcaccaac
     5401 cagaacgcga tgttcaacgg gacgcccctc atcgcccccg ccgagccgct gcgcgcctac
     5461 tcgttccgca agcgctttgt cgccgagtac gtccccatgg gcatgggcat cctcaccgcc
     5521 ttcatcggcc tcttcgcgct cacgatgtat ctcttcgacc ggcgctcgcg cctgccgctc
     5581 tatctggccc tgacgtcctt tctctggacg atcatgtcca cgcatttctt cgtgcccgac
     5641 ttcgtgatca tcgacttcaa cacgcacgac aagctctact acgcgctcat cagcatcgag
     5701 atatcgctga tttatctttt cctcgaggac atcctctcgg tgcgcatcaa ggccatccgc
     5761 atcatcgtcc tcgtcctggc gggactcagc ttcctcgtct ccgtgtccgc caccgcggag
     5821 agccccatca cgggctggcg cttcgacgtg atcggcggcc tcgggatcct gagccaggtc
     5881 ttctgggggg tgctcatcgt gcgcgggctt cgcaagagaa cgccggagag ccgcatcgtc
     5941 ttcatcttct atatcctgtt catggtttgc ctgctccacg acagcctggc catctcgaac
     6001 ctcatctcct acgagtcctt ctggatcaac ctcgcctatc cgtcgctcat gatcgccttc
     6061 agcatcatcc tgtcgatgca gacggcggtg atggcgcgca agctccagat ctcgagcgtc
     6121 gagatcgagg agaagaaccg cagcctctcc gagatgctcg ccggcgtccg cgagagcgcc
     6181 tcggagctca cggagttctc gcagacgctc cgcaagaccg cgaccgacct ccagaacgac
     6241 atggacgaac aggggaccaa cctggaccag acggccgcgg tgatcgagga ggtctccgcc
     6301 tcgatcgagt ccgtggccga caacgccgcg cgccaggaca acgtggtgcg ctcgaaccag
     6361 gacctcctgc tcgagtacgt cgactcgctg cgcaagatca ccgaggcggc gaaggacgcg
     6421 gcgaagctga gctaccgcag ccagggccag accgccgaga gccgcgccaa gctcgacgac
     6481 atcaccgccg gcatgaacaa gatcaagcag tcctccggcg cgatcaacga gatcaccgag
     6541 atcatcaacg acatcgccga gaagacgaac ctcctgtcgc tcaacgcctc catcgaggcc
     6601 gcgcgcgccg ggcagtacgg ccgcggcttc gccgtcgtgg ccgacgagat cggcaagctc
     6661 gccgacagct ccattcagca ggccaagacc atccagggca tcatcgccgg caccgtggcc
     6721 gacatcgagc gcgagacgga gctggtggcg cgctcatcgc gctccatcga cgagatcgag
     6781 aaggccgtga acgacgtgaa ccgcgggatc gacgcgatcc tggacctgtg cctcgcgcag
     6841 gagaagctca ccgaggagat cggccacaac atgaagcgca tcctcacggg ctcatccaag
     6901 atctcggcgt ccacgcagga ggagaagacc tccatcgcgg aggtctcgaa gtccatcgag
     6961 cacctcaccg gcatcatgga gaccgtcaag gagagcgccg cgcaggtgat gagcgaactc
     7021 gagcggctct acaagcgcat cgagattttg cacgcgctcg taaaggacta ggcgggcgcg
     7081 atcggccatg gccatcaagc acagacgggg caatgcgctt caatccaaac gattcatctc
     7141 aaccctatcg agtacagacg aggcaatgcc ccgtttcaac cccaccgcat tggattattg
     7201 gcgatataat tccgaaccgc ccgcaaggac tcttcatctc gtatgatgca atcataaaat
     7261 cgcgattgcc atcggaatcg ctcgccgccc ggcattcgat ggatcatttt tgcgcatacc
     7321 gatttatacg atccgataat cgatgatatc gtcccgcgtt ttcgtttcgt ttccatatgg
     7381 gtgtcgcgat gggtgtcggg ttcgttatgc acgtgggttt ggtgacgggc gttggtttcg
     7441 ttattttcgc gggttcggtg atgggcgttt tgtgacgtag agacagggca atgccctgtc
     7501 tctacgtcga tggcgccatg tcgcgcaacg acggcatcgt gccccacaac gatggcgtca
     7561 tgtcccgcga tgtcggcatg atccaatccc gcccatatgg atcgatcgat aatgattatt
     7621 ccgtgtacat ggtccggcat tacgacaaac gcatccaata gtacaaatgg gaaatgatgg
     7681 ggtatttcat gccaatagcg ttcggcaatg gcgccaaagg gatttaagcg catgattcgg
     7741 ttttccacac acccgaacgc acgaacacga tcgtgtacac acatggtgat aaaataacag
     7801 ccgttcgcgc cgtaatccca ctgtttcaaa cgcgttgacg caatgcgata ttttccctga
     7861 tagcggtcca catactatat acgaggcgcg gggggaactg ttagtttttt tcgagataaa
     7921 tcgaaaccct tcaccggaaa tccggggcag tatattcgct tttctcatgc tggcaatccc
     7981 aacctgccac gcttgcgacc tgccatcatc aaaggcgata caccccttgg ccggcagggg
     8041 cgcgtcgtgg accggaccga tgaagacggc agaccgggca tcggaagctc tccgtgatgt
     8101 tcgccgacgg cagccagcag gccgggatca tctacgactc ggcggaaaac gcctgtccga
     8161 cgcggtcgcc ataggactcg aaagggccat cccgcgcctg ccggcgcgat cacccccata
     8221 aaaaaacgcg tgacgccatg atttatatat tcatctttgg ctatgcaatt tttatggttc
     8281 cgcggcggga catgattcga cgtgttgcgc aacccgacgt cattgcgagc gaaacaacgc
     8341 gatccgaaaa aataaacaac ccacgccacc cggatgtccg gcgaaagagc gcgccgttcc
     8401 gataacggcg ccggatcgca tcaccgcagc cccacctcgc gatgaccgga aaccctgttt
     8461 cgcaacagtt cgacgtttcc cccgccggga ccagcagaca aaacatcatt tacacggagg
     8521 actccttcac atgaaaacac ccatgtattg ctcgatgaac cgcttcattc gctctgcttc
     8581 aacacgggga tgtttcgcca cgcgccgtgc gtttttcgtc tttatgattg cggcgatcct
     8641 tgccggattg acgcttcctt catgcaacac gtccagctgc cagtgggtta cgacgggctc
     8701 ccgaaaggga aaaaagacca cggaatacgt gtgcggcgac aaactgaagc ggattcaaag
     8761 aaccaggcag aacaacgccg ccgcgcgaaa cagaagcgtc gaagagaaca atgccttcac
     8821 cagcaagctc gccgccgccc ggccctccgc ggaccagcag gtgttcatcc agctcgtcaa
     8881 ggccgaacag gcggagaaag cccggatgaa gggacagacc accgccgccc aggaggcgat
     8941 agcgaaaagc atacaggcaa aatacaagaa ccggcccata tcgttccggc aggtatgctg
     9001 cgtcaacgtg gaacgaaaat tcaataccta taccgcggta ttttatgttc ccgacccgaa
     9061 cgcgggcggg ggatgcaccg gaaggccggt ggacgaaaac tttaacttct acggggtccc
     9121 cttcgtcgtg aaacggaacc tttccgcggc cgcggcggga cgctataaga tcggcacggc
     9181 ctattccgta tcgggccgtc ttgtagtgaa tgaaaactcg tttagcaccg actacgaaga
     9241 gaatcagtac ctgctcttaa gaggcgttcg gccctatgaa gcgggcaact ggagttccaa
     9301 gacctacccc gagggcggca aacgtctcgg gggtcccgac caccagcgtc gaaccgacgt
     9361 acggaaatcg tattatggtg aattcttcac cttttcgcaa atcatcactc tggactgacc
     9421 tcctcccgcc ctcccccttc gttaggggga gggcgtcttc cgtgcccccc gttcccccaa
     9481 cgaaggggaa cggggccggg ggatagtggt caagcacccc tatcgtactt gacattctgt
     9541 ttttcccgca cccaatgggg caatgccaat ccacccatca tccatgcaaa ggctgtgcgc
     9601 cgcattggtc ctgtgcgtcc tcgcctggac tgcatgcacg ggctgcgggc cgctgtcctc
     9661 gaaggagaag cggcccgtcg cggtcaaggg cgtcatcgac cttcggaact gggacttcga
     9721 gcgcgacggc gcaatcaacc tgaacggcga atgggagttc tgctgggacc gggagcttgc
     9781 gccaaacgat ttctcgcgaa ctgccgggaa aaccgactgc ggtttcatca ccgtgccggg
     9841 tccgtggaag gaacagacgt acaacggcgc acccctgccc ggcgaaggcc gcgccaccta
     9901 ccggcttaaa atcatcaacg gccccgatgc caagtcaaag gccctgaccg cccggggcat
     9961 cttctccgtc tacaagctct gggtaaacgg cgtcctgctt gaccgccggg gggattccgg
    10021 acgggcagcc aagacccggg aagactacat ctatgtgcac aacaggcacc tttcgtcgtt
    10081 cacgctgcag gcgggcgtga acgagatcgt cctgcaggtc ttcaaccggg accatgcgag
    10141 cggcggcata aaccggcagc tgaggatgga gaacgggagc accgccgcgc gaaacgaatt
    10201 ctaccggctc aacatcatta tggtcatcgt gggcctcacg ctgttctcgg cgatctacaa
    10261 cgtgctgttc ttcctcttcc ggcggatcga cccgacctcc ctgtatatcg gactcgccag
    10321 cctcgtctcc gccgtgaaca cgtacaacgt ggagcatccg atcctgacca gcggcctttc
    10381 ccctacgggc aatcccttca tcatcaactt cgttacggtc atcgccggaa tgctgctctt
    10441 catgatgatc gcctggtcgc tgttccccga tgatttttcg aaatcgatca tccggttcgc
    10501 ccaggcggcg tcggcgtgtt tcatcgttcc gctttttttc gttggcttca aaaccgcgca
    10561 atcgatcatg tatttatttt tcgcatttac cgtgatgctg ttcacttacg gcctgtttgg
    10621 gctatgcaaa gccatcatca acaaacgaag ggacgccctt ctttttttcg caagcctgtg
    10681 cccaatctat atagcggccg ccaacaatat tttgtattac ctgcaggtca tcgacaccgg
    10741 caacctggtg ccacacgcca tggtgctatt ctgcatttcc agcaccattg tcgtctcccg
    10801 tcgtttcgcc cgggcgctgg ggaccgtcga ggaaatgtcg cgggagctca cggagaagaa
    10861 catcacgctc gagaggctgg accggctcaa agaccagttc ctcgccgcaa cctcgcacga
    10921 actgcgcacg ccgctgcacg gcatgattgg cctctcgaac tcccttgcca acggcgacac
    10981 gggccccctc cccccgaagg ccgtcgagac cgcctctctc attgccgcga gcggcgctcg
    11041 tctcgcggca atggtgaacg acttgctgga catggcaaag atccaggaag agggtctttc
    11101 gctcgcgccg cggcctgttg acctgcacgc cctgagcgag acggtggtgc agctctcgct
    11161 tcccctcgcg ggcgacaagc ccatggagat cgcgaaccgc atcactccgg atattcccgc
    11221 cgcctgggcc gacgaggacc gcgtacgcca ggtgctctac aatctgctgg gaaacgcgat
    11281 caagttcacg accgagggga ccatcgagct ctcggcgcgc gtgatgcgcg tgtcaaacgg
    11341 cgatcacgag ggcgacgctc gcgccatgat cgaggtgcgc gtatccgaca ccgggatcgg
    11401 cgttccggag gagtaccggg agagcatctt cgaggcctac cgacaggccg atgcgggcga
    11461 tacgcgctct tacggcggca cgggactggg tctggccatc gcgaagcagg tcgtggaggc
    11521 gcatggcgga tcgatcgcca tggccccccg cacgggcggc ggctccgtgt tctccttcac
    11581 cctgcccgtg tcgactgcgc caacgcgaga cgatgaaacc gcgcccacca tgacggacat
    11641 gcccggcgat gcgccgacgc ctaacgagac gggaccgtcc caccttcccg ccgggatcaa
    11701 caagaacgac ctcggaaaac gtccggtaat ccttgtcgtg gacgacgaca cggtgagcct
    11761 gcgcgttgtt caggattttt tcgagtcgcg ggacataacc gtaaagaccg catcgaacgg
    11821 catcagggcg ctggacctca tcgaaaagga cgattccctc agcctcgtcc ttctggacat
    11881 catgatgccc atcatgtcgg ggtacgacgt gtgccggcgg atccggattt cccgctcgcc
    11941 cgaggagctg cccgtgatca tgctcacggc caagaacatg atggccgaca tcgacgccgc
    12001 cttcgcggcc ggggccaacg actacctcgt gaagcccttc cagccctccg agctcctggc
    12061 gcgcgtgagc accatgctca agctcaggaa ggtgagcagg gggacggccg agggcattac
    12121 cgtccgcgac cggatacggg cctactccat cgccttcggc gacattaccc atatcacggc
    12181 gcattcgaag aatgtcgtga tccatacgac cgagagcgat atcgcgatcc caaccggact
    12241 tacggagatc ctggaccggc tcccgccgga cctctttgtc cgcatccaca agagtcatgt
    12301 ggtgaacctt cggcacgtgc acagcgtgtc ccacgtggtg tcgggacgct atcgggtgcg
    12361 cctccgcgat aaggacgaca ccgagctccc cgtggggcct gcccacctcg ggccgctgcg
    12421 gacacggatg taacgccggt gcgcgccaac ggcgcgagag gaactcatcg gccgtgaggc
    12481 cggaaccctt gatgatgccg cccccacgca cagttcgctc cccgagaggc acgggttgta
    12541 ccgccgcgtc ttgacccatt cccacggcgt tcctatactt gtacatattc gctatcgcat
    12601 agaaaatgct ctattcattt tacacgatca tacgaggcgc cgcattggtg ccgtccgaca
    12661 ggtgcgcatg tccggcgcga tcttggcgca cgtatggatg gggagcgatg gggattttga
    12721 gacggggcgg cgcctcgtct ctacgggcat cgcataccat aatcgtaata ttcatttgca
    12781 tcctggcatt ttcccttccc gcctgtaatc gggacgtgta cgataatctg ttcgatccgg
    12841 taaaccaata caccgtgacc ttcgactcgc agggcgggac ggcggtatcg gcccagacgg
    12901 ttgactacgg gaccaccatc tcccagccgc ccgaccccgt gctcaccggc ttcaccttcg
    12961 ccggatggtt cgcggagccg gggtgcacaa ctccctggct cttcgggagc ggtaaggtcg
    13021 agggcgacac gacgctgtac gcccgctgga ccataaacca atacaccgtg gccttcgatc
    13081 cgcagggcgg aagcgcgatc gccccgcaaa cggtaagcca caacggcact gcggccgatc
    13141 ccggcgcgcc tgcgcgcacc ggctacacct ttgggggctg gtttcaggaa gccggatgtg
    13201 tcacgcaatg ggtcttcgcc atcgacaccg tcaccgagaa caggacgctc tacgcgcagt
    13261 ggaccatcaa cacctacacc ctcgcatata ctgcgggcgc aaacggctcg ctcacgggat
    13321 cgaccacgca gttcgtcacc cacggagcca acggtacggc ggtcacagcc gtaccgaacg
    13381 tcggctatca cttcacctcg tggagcgacg gctccaccga caatccgcga acggacaccg
    13441 gcgttaccgg caatataacc gtcacggcaa acttcgccat caatcaatat acgctctcct
    13501 actcggccgg ggccaatggt tcgctctccg gggcctccgc gcaggtggtc aatcacggtt
    13561 cggacggcac cgccgtcact gtcgtcccga acgtgggcta tcacttcacc tcgtggagcg
    13621 acggctccac cgacaatccg cgcacggaca ccaacgtcac gggtaccatc accgtgagcg
    13681 cgaatttcgc gatcaaccag tacacgctca cctacacctc aggcgcgaac ggcacgattg
    13741 ccggaaccac tccgcaaacg gtcaaccacg gggcaagcgg ctcggcggtc acggcaacac
    13801 cgaacgtcgg ataccacttc acctcgtgga gcgacggggt gcttaccgca acgcggaccg
    13861 acacgaacgt gaccggcgcc ataacggtta ccgcgtcgtt cgcgatcaat acctataccc
    13921 tcacctacaa tgcgggcgcg ggtggttcca tcaccggatc aagtccgcag accgtgaacc
    13981 acggatcgaa cggcaccgcc gtgacggcca cgccgaacgt gggctatcac tttacctcgt
    14041 ggagcgatgg cgggcttatc gccacgcgga cggacacgaa tgtgaccggg gatatgacgg
    14101 ttatcgcgaa cttcgcgatc aacacgtaca cgctcaccta tactgcgggc gcgaatggat
    14161 ccataagcgg cacatccccg caaacggtga accacggttc ggacggcact gccgtgacgg
    14221 cgacgccaaa tctgggctat catttcgttg actggagcga tgggtctacc acaaatccgc
    14281 gtacagacac aaacgtgacc ggtgccatca tcgtgagcgc aaacttcgcc gtcggtggaa
    14341 ccctcgcggc gtcgaccttc tcggtggatt cgggatcacc atgttgggat gtcgcggtca
    14401 atcctggcgc gaataccgtg tatgcatcat gcggcaatcg cctgcatctg ttaaacgccg
    14461 ccactggggc cacgatagga atcttcgcaa catacaacgg tgtgggagtg gccgttaatt
    14521 taaacacagg tcttgtgtat gttgtaaata gcagcatttc tgcgggcgtg acctttttta
    14581 attctgacgg gacgtatcgc ttcggcaccc ttgcggcatc gaacttcccg actggattat
    14641 ttccccatca cccggcggtc aatccgacaa cgggtatttt gcatgtaact aattataact
    14701 ctaacacggt gacctatctc gatgccgcca ccggagggtt gattggaaac tacccaacgg
    14761 gggcgaaccc ctggggtgtc gccgtcaatt cgacgacaaa cattgtgtac gtgacctctg
    14821 acagcagtag cagcaaaaga tacctcgacg cctccagcgg agcccttctg ggagattatg
    14881 caatcggatc caactcctgt atggtggcgg taaaccccgc cgcaaatatc gtatatttta
    14941 caaacacaac cgcaaatacc gttacctatc tcaacgcaac caccggagca ctcatcgggc
    15001 attttacaac cggatcaaat ccgcacggcg ttgcggtcaa tccc
//
LOCUS       DAORHF010000071        14996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3042_length_14996_cov_5.672244, whole genome shotgun sequence.
ACCESSION   DAORHF010000071 DAORHF010000000
VERSION     DAORHF010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14996
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3042_length_14996_cov_5.672244"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PKJ16_07840"
     CDS             complement(<1..271)
                     /locus_tag="PKJ16_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HNV46936.1"
                     /translation="MARSAPVVEDMPGVAQIPGENVRARAFVKKIILLRIRRVERTPD
                     VMERFHMAPMAEITTPALRRAIRRYSHGAVLYSEMLSAGAIAAGSS"
     gene            293..754
                     /locus_tag="PKJ16_07845"
     CDS             293..754
                     /locus_tag="PKJ16_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46937.1"
                     /translation="MAKLRAVYDGTLYLYFTIITLVIALALGAVSYYSIWRVEPAIER
                     HLDAEENVNENLRKAYAMLLDPQIFGRYENFDLIGAPIKNIIADYDKKLAANEPFVKE
                     DRMYLEVLFERRRMGSLLGRNTMIFFFLLSALGAGVFAYERMRYGSKRGAQ"
     gene            751..1698
                     /locus_tag="PKJ16_07850"
     CDS             751..1698
                     /locus_tag="PKJ16_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV46938.1"
                     /translation="MTISRPFAIKAVCALVVLAIGIYGVANYHADIPADVIKKKYTDA
                     ESKFITVEGIEVHYKDEGVGLPVVLLHGMSSSLHTWDGWIKDLNEWYRVVRLDIPPFG
                     VTGPNKTGDYSIDWYDKFLTAFLDALKIDKCFVAGNSFGGRLAWEYAVRHPERVRKMI
                     LIDAARYKRSLPAPVRLARVPVLSSVMTKITPRSIVSNAVVGNYGDRSKVSDALVQRY
                     FDLTLREGNRAGYGDWARALRHNETTSLSKLYIPVLIMWGAKDPWIPVEDAGRFKLEL
                     PDARVIVYPEAGHMPMEELPEKTAKDAYHFFRGSIQDPR"
     gene            complement(1754..2131)
                     /locus_tag="PKJ16_07855"
     CDS             complement(1754..2131)
                     /locus_tag="PKJ16_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46939.1"
                     /translation="MIPDVLFWLYFANAILIITHEIDSGYWREWELFGMRGGIDLFLL
                     LHIPMLGAILYGLIEAHRGTMTGAVFSLILGGAGIFAFCIHTYFIKKGRPEFRGMMSQ
                     AILHATLVISLLQTCLTLYRLLA"
     gene            2304..2711
                     /locus_tag="PKJ16_07860"
     CDS             2304..2711
                     /locus_tag="PKJ16_07860"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV46940.1"
                     /translation="MAFINWNKQYSVGVKLFDDQHFKLVEMANALYEAFKAGRGREAL
                     GETFKGLVDYVQVHFTAEEELMLKHEYPQLLRHRQEHIELIARIKDLYGKALSGSSAV
                     SMEMLNFLRDWLIRHIAESDRQYGKFFNDRGVF"
     gene            complement(2736..3194)
                     /locus_tag="PKJ16_07865"
     CDS             complement(2736..3194)
                     /locus_tag="PKJ16_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46941.1"
                     /translation="MKRATRKYPLILLAFTLAAATPGDILKRYDMDPRQLQTVYQCIK
                     AANFREVRDFSAVTTEGGRYRFQSEKRAAIVYFKSKQLDELRTAIGGDEVFIALLRNA
                     ACYGVFIETKPMKDADGKENAYSVFDAHVVVIEHGSGADEITVVCMADRT"
     gene            3343..3861
                     /locus_tag="PKJ16_07870"
     CDS             3343..3861
                     /locus_tag="PKJ16_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HNV46942.1"
                     /translation="MPLYEYNGKRPTIGAGTWIAPNAHVVGDVSIGANSYVGFGAVIR
                     GDFGTVVIGDETAVEEGVVIHTSTSCVIGSGVILGHLAMVHGARIDDATVIGMQATLC
                     NESVVGSWTIVAEQSLVVKGQTVPGGAIVGGSPAREIGALTERHRAYIVHARRVYAEL
                     ARGFAEGLRVVG"
     gene            4106..5125
                     /locus_tag="PKJ16_07875"
     CDS             4106..5125
                     /locus_tag="PKJ16_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HNV46943.1"
                     /translation="MRMRIFAVLVLSLAVAVSACSKSPDPGEVITLSYSIFFPATHEQ
                     CIAGMNWAKEIEKKTDGKVKINVYPGGTLTKADETYNGVSAGIADIGMSCFAYTRGRF
                     PIMEAVDLPLGYPDGKTATAAANEFFKAMKPKELDDVKVLYLHAHGPGLLHTKKPVKK
                     LEDLKGMKIRSTGLSAKVVSALGGVPVAMPQGATYESLQKGVVDGTFAPIETLKGWKQ
                     GEVIKHTTNSRTIGYTTGMYVIMNKAKWDKLPDAVKAVFTDVSDRWVKVHGETWDRVD
                     GDGVAFTKNLGNGIIELDAAEGARWSAAVKPVIDGYIAEMKKKNLPGDKAIAELRRLI
                     AQSGK"
     gene            5182..5664
                     /locus_tag="PKJ16_07880"
     CDS             5182..5664
                     /locus_tag="PKJ16_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HNV46944.1"
                     /translation="MNKLEYYLTRISDSYNVVAAAAVVLMMLVIIVDVVLRIVRVSVP
                     GAYDLISLLGAVAIAFSLTYTSIQKGHIAVDFLYQKLPERWQAGFDVFNESVGAGFFA
                     VLAWQCFAYAASLRAAGEVSLTVKLPVYPFVAGIGASCALLSLYLLLNLAKAIRRMVK
                     "
     gene            5664..6965
                     /locus_tag="PKJ16_07885"
     CDS             5664..6965
                     /locus_tag="PKJ16_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HNV46945.1"
                     /translation="MTPTTVGLVGLFALVALLFTRMPVGFIMAVVGFVGFAAIVSFDA
                     ALKLVANDVYSVFSSYNLTVIPLFVFMGQLAFHSGISTRLFDAAYKCTGRLPGGLAIA
                     TIGACAGFSAICGSTNATAATMAAATLPEMKRYNYKPGLATGVVAAGGSLGILIPPSV
                     VFIVYGILTEQSIGKLFIAGIIPGILLTLFFIAAVLIWTTLYPDQGPPGPRTSLKEKL
                     VSLAGLGEALVLFSMVMGGLFAGIFTPTEAGGVGAFGTLAISLARRKLDWKGFMAALN
                     ETTRISCMIMVIVAGATIFGHFLAVTRIPFDIAQWISGFDMHRAVIMLMIIAVYVIGG
                     CFIDALALIMLTIPIFYPVVLHLGYDPIWFGVVIVMVTQIGVITPPVGINVYVVSGVA
                     RDVPLEVIFKGVVPLLVALILATLLLIPFPQIALFLPGFVK"
     gene            complement(7005..7460)
                     /locus_tag="PKJ16_07890"
     CDS             complement(7005..7460)
                     /locus_tag="PKJ16_07890"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV46946.1"
                     /translation="MTAFQITIPAENKPGTLLRVTSVLSKEKINIRAITITSFGGSGF
                     FHLVVDDPKLAHAALEKAGIANQLKEVIAVLIDDAPGSLDRLIQRLFEKNVNVENAYG
                     FVLESRQKAVFVIDVQKIEEALRMLAAEGYKTLDADALNAVEPFHYMKY"
     gene            complement(7596..8315)
                     /gene="rlmB"
                     /locus_tag="PKJ16_07895"
     CDS             complement(7596..8315)
                     /gene="rlmB"
                     /locus_tag="PKJ16_07895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HNV46947.1"
                     /translation="MEILGRKPVLEYLRSLDTGAGIELFVSKSAHGKIIDEIVAAAKR
                     IGARAELVEKAHFARHPSSTHQGVMLVSRRKRETVTDAEFVAEVSARGGVLVLLDHLT
                     DPHNVGAIIRTVEALGGDGVALTRHGTAEVNATVVKASAGATAHLRVITTPNAAHFIE
                     LARERGFSIVGTAADGTIGLDELPGLRPLVIVIGSEGEGMRRLTEERCDHIVAIPLRG
                     MVASLNASVAAAIVLHEALKE"
     gene            complement(8319..9749)
                     /gene="cysS"
                     /locus_tag="PKJ16_07900"
     CDS             complement(8319..9749)
                     /gene="cysS"
                     /locus_tag="PKJ16_07900"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HNV46948.1"
                     /translation="MPIIVHNTISGLPDEIVPGGARKDRVEDYPPVSIYSCGPTVYGY
                     AHIGNFRTFMFNDFLRRYLRFRGYAVNHAMNITDVDDKTIAGAQKDRITLREYTEKYT
                     NIFLEDLRALNIEPVEHMPRATESIDAMADIMERLRERGFVYEKDGSVYFSIAKYERY
                     GRLSRLDTREIKSGLRYDTDEYEKDDVRDFALWKATAPNEPSWDTPFGPGRPGWHIEC
                     SAMVRKIFGGTIDIHTGGVDLIFPHHENEIAQSECAYGEPFVRHWIHVEHLLVEGKKM
                     SKSLGNFYTLRDLLEKGHSPRSIRYLLISAHYRKQLNFTFDGLRQADAALDRIDNLIV
                     RLGDAKGDGPVAAEVSGAIDSFLAKFAATVDDDLNISGGMGHFFDFLHEVNALIDRGT
                     MTAAGRDAVITALRALDAVLGFIFFPAQADAGVDAARIESRIAERAAAKKAKDFARAD
                     LIRDELLAEGIILEDTKDGVRWKVKK"
     gene            complement(9776..11311)
                     /locus_tag="PKJ16_07905"
     CDS             complement(9776..11311)
                     /locus_tag="PKJ16_07905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV46949.1"
                     /translation="MTTSPKRSAARLVRIARLCCIALACAAACRGERPTDHFNTYIQS
                     DPARLDPFYSTDVVSGRVMAHLFNGLFKISSNGSLERDLAATHRFDGTRLEATLHDGV
                     RFHDGTALTAADVAYSLDRVRLSENPTSPRRWLFANIARIATPDERRIVITLREPSAS
                     FPHLLATPTAYIISAAAHRRDGSVVGTGPFALREWRQDERLVLARHERYFEGPARVSG
                     IVYRIIPEDLTARFEFLNGTLDYFEVPYLARVRHDAQARTIDVPEYSVHYIAINNARP
                     PLDNPALRAALNRAVDRRRIMRALFDNRFTLAAGPVPNTLAGHRSAARPYDYDPVAAR
                     AAIASLGLSGHRITLFIKSDHQVSLIAQMVQRDLAAAGLAVSIREMEWSALKAATLNG
                     AYDLCYFTWHADYPDAENFLYPLFYSKNIGQGGNRAFYRNVSVDRLLDEARRTMDAGK
                     RIDIHRRAEQLIVSDAPWIFLWYGDKRVMLGRRVRSFVPYPLYNGMKGHEIELEPDGR
                     TTR"
     gene            complement(11289..11819)
                     /locus_tag="PKJ16_07910"
     CDS             complement(11289..11819)
                     /locus_tag="PKJ16_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46950.1"
                     /translation="MIKRPKKPTIVPDFTEFTDTEGTKWVQTSADDLFSIKEINKILE
                     SIEKDKDEGDIVIARIDFQKVNKEYYERVRELQTKLKKKNELIKRLLMGSKETIDKKN
                     RKLKELIEYIKKLHLLIAAYRQDPERMRDIDLPALTPVEPEPVPVVAIERVAEIPAEE
                     FVEVEEVVLGDNEPEA"
     gene            12073..12990
                     /gene="murB"
                     /locus_tag="PKJ16_07915"
     CDS             12073..12990
                     /gene="murB"
                     /locus_tag="PKJ16_07915"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HNV46951.1"
                     /translation="MIDPDILKELAPFGRVREYAPLREYTTFRAGGPADALVVPNTAA
                     DVPGIVRVVRERALPLTVIGGGSNLLVGDRGVRGVVLAIRERDEAAGAIAVSDNGDIA
                     AEASVSKERFIDFAIANGFGGVEFMAGIPGCLGGGIIMNAGTTLGWFADILVGIRIVD
                     GAGALRDLPVTKEMSTYRRLRIDDDSIIIAGAFRLPRAEDPASVRETIAGLLRERAGK
                     HPLDYPSAGSVFKNPEGHSSWKLINDAGLKGTRIGGAMVSELHTNFIINYDNATARDI
                     RDLIEHVRETVQAKFQVRLETEVRMLGEF"
     gene            complement(13427..14173)
                     /locus_tag="PKJ16_07920"
     CDS             complement(13427..14173)
                     /locus_tag="PKJ16_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46952.1"
                     /translation="MKILHVAIAFASISLAAYSTPALAQYHIDLEGGYVDTGYNDIRI
                     PAKGGTRFSLTQDLEAQPTYFYRGRAGYTFSDRHTVSFLAAPLRVVSLGTLRKDVRFG
                     YTTFREGEYVETTFRFDSYRVTYRYDFFTNESWRIGAGFTAKIRDASIKMESADLIAE
                     EKNTGFVPILNFNIEWKFAGPFSLLVNGDALASPYGRAEDVFAGFLYQASKNVIVKLG
                     YRILEGGADNKTVYTFSQFNYAAAGVVILL"
     gene            complement(14285..>14996)
                     /locus_tag="PKJ16_07925"
     CDS             complement(14285..>14996)
                     /locus_tag="PKJ16_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46953.1"
                     /translation="VVYTSDDAGAAWMLRFTAPTCAVFRDVATSRSGAWIAVGQNFYA
                     RSTDNAATWTEYSSFDIYNITYANGMFATVGSGGTAYRFSTNDGASFTPASSAPAIAS
                     DGLIRGNSRFVACGPTVTYFYSTDADTWVPQAAGIITSNIYHLSHDGKQRVMAAAHPN
                     PVIYFTDDDGANWTPKTLTTPTLWGIAYGEGKWVAVGFNAWAAVCLSTDGGNTWNEQQ
                     LSGTGITTPLRAVAFGYR"
BASE COUNT         2663 a         4616 c         4812 g         2905 t
ORIGIN      
        1 tgctgctgcc ggccgcgatc gcgcccgccg agagcatctc ggaatacagg accgcgccgt
       61 gcgagtagcg gcggatcgcc cgtcgcagcg cgggcgtggt gatctcggcc atcggcgcca
      121 tgtgaaaccg ttccatgacg tcaggcgtgc gctcgacgcg gcggatgcgc aacaaaataa
      181 ttttcttgac gaatgcgcgc gctctgacat tctcccctgg tatttgcgcg actcccggca
      241 tgtcctcgac gacgggcgcc gatcgcgcca cactacctcg caggacacaa tcatggcaaa
      301 gcttcgcgcc gtatacgacg gcaccctcta tctgtatttt acgatcataa ccctcgtgat
      361 cgcgctcgcg ctgggcgcgg tgtcgtacta ctcgatctgg cgcgtcgagc ccgcgatcga
      421 gaggcacctc gacgccgagg agaacgtgaa cgagaacctc cggaaggcct acgcgatgct
      481 gctcgatccg cagatcttcg gccgctacga gaacttcgac ctcatcggcg cgcccatcaa
      541 gaacatcatc gccgactacg acaaaaagct cgccgcgaac gagcccttcg tgaaggagga
      601 ccgcatgtac ctggaggtcc tcttcgagcg ccggcggatg ggctcgcttc tcgggcgcaa
      661 tacgatgata ttcttcttcc tgctttccgc cctcggcgcg ggggtgttcg cctacgagcg
      721 aatgcgctac ggatcgaaga ggggtgcgca atgaccatct cccgtccgtt tgcaataaag
      781 gccgtctgcg cgctcgtcgt tctggcgatc ggcatatacg gcgtcgcgaa ttatcatgcc
      841 gatattcccg cggacgtcat taaaaagaag tacaccgacg cggaatcgaa attcatcacc
      901 gtcgagggga tcgaggtcca ctacaaggac gagggggtgg ggctccccgt ggtccttctt
      961 cacggtatgt catcgtcgct gcacacctgg gacggatgga tcaaggacct gaacgagtgg
     1021 tatcgcgttg tcaggctcga tataccgccc ttcggcgtca ccggccccaa taaaaccggt
     1081 gactactcga tcgactggta cgacaagttt ctcaccgcct tcctcgacgc gctcaagatc
     1141 gacaagtgct tcgtggccgg gaactcgttc ggtggtcgcc ttgcgtggga gtacgccgtg
     1201 cgccaccccg agcgcgtgcg aaagatgatc ctcatcgacg cggcgcggta caagcgttcg
     1261 ctgccggccc cggtgcgcct ggcgcgcgtt cccgtgctta gttcggttat gacgaaaatc
     1321 accccgcgaa gcatcgtttc aaacgccgtc gtgggcaact acggcgacag gagcaaggtg
     1381 agcgacgcgc tcgtccagcg gtacttcgat ctcacgctgc gcgagggaaa ccgcgccggg
     1441 tacggcgact gggcgcgcgc cttacgacat aatgaaacca ccagcctttc gaagctgtac
     1501 atcccggtgc tcatcatgtg gggcgcgaag gacccgtgga tcccggtgga ggacgccggg
     1561 cgattcaagc tcgagctccc cgacgcgcgc gtgatcgtct atcccgaggc cgggcacatg
     1621 cccatggagg agctgcccga aaagaccgcg aaggacgcct atcacttctt ccgggggagc
     1681 attcaggatc cgaggtaagc ggtcgggttg ctctcgtgag gccaatggag agcaaccgaa
     1741 agatgcctgg cgatcaagcg agcagccgat acagcgtcag gcaggtctgc aggagcgata
     1801 tcaccaatgt tgcatgcagt atcgcttgcg acatcatccc cctgaactcg gggcgccctt
     1861 ttttaatgaa gtatgtgtga atgcagaagg cgaagattcc cgcgccgccc aggatgagcg
     1921 aaaacaccgc gccggtcatt gttcccctgt gcgcctcgat gaggccgtag aggatcgcgc
     1981 ctagcatggg gatgtgcaac agcagaaaca ggtcgatccc gccgcgcatt ccgaagagct
     2041 cccattccct ccagtaaccc gagtcgattt catgggtgat gatgagaatc gcgtttgcga
     2101 aatacagcca gaaaagaacg tccggtatca tgacgaccct cccgcaacgt gttgatggcg
     2161 cgcccggttc cgttggtagg tcgtcggcgc gtttttcgca agtaaaattg cttgtactgt
     2221 ccggtatcgg tgcgtatcat catcgcgcat ggaaaagttg aacccggcat gacgcgcgct
     2281 atgctgtatc atggaggagt tatatggcat ttatcaattg gaacaagcaa tacagcgttg
     2341 gcgtgaagct cttcgacgac cagcatttca agctggtgga gatggccaat gccctctacg
     2401 aggccttcaa ggccggccgg ggccgcgagg cgctcgggga gaccttcaag gggctggtcg
     2461 attatgtcca ggtccatttc accgccgagg aggagctcat gctcaagcat gagtacccgc
     2521 agcttcttcg gcaccgccag gagcacatcg agctcattgc gcggatcaag gacctgtacg
     2581 gcaaggcgct ctcgggatcg agcgcggtga gcatggagat gctcaacttc ctccgcgact
     2641 ggctcatccg gcatatcgcc gaatcggacc ggcagtacgg gaagttcttc aacgatcgcg
     2701 gcgtttttta aacgaggcgt gcgttccgcc ggacgtcagg tccggtccgc catgcacacc
     2761 acggttatct cgtcggctcc cgacccgtgc tcgatgacga cgacgtgcgc atcgaaaacc
     2821 gaatacgcgt tctccttgcc atccgcgtcc ttcatcggtt ttgtttcaat gaacacgccg
     2881 tagcaggccg catttctcag aagcgcgatg aaaacctcat cgccgccgat cgccgtccga
     2941 agctcgtcga gttgcttcga tttgaaatac acgatcgccg cgcgcttctc cgactggaat
     3001 cggtagcgcc ccccctccgt cgtcaccgcg ctgaaatcgc gcacctcccg gaagttcgcc
     3061 gccttgatgc actggtagac cgtttgcagt tgccgggggt ccatgtcgta tcgcttcagt
     3121 atgtcgcccg gggtcgcggc cgcgagcgtg aaggcgagga ggatgagcgg gtatttccgg
     3181 gttgcgcgtt tcatgatgat aagatacttc ggtatcgatc ccggaatgtc aaatcgattt
     3241 tcctggcggc gaatattcga caatccgcct tgtgaacggg gaggcggccg gcgttccaaa
     3301 aggcgtcggc acattgcatc ggaatactga acggagctca tcatgccatt atacgaatat
     3361 aacggaaagc gcccgacgat cggcgcgggg acctggatcg cgcccaacgc gcatgtcgtg
     3421 ggggacgtaa gcattggcgc gaactcgtat gtcgggttcg gagcggtcat ccgcggcgac
     3481 ttcgggacgg tcgtcatcgg cgacgagacg gcggtggagg agggcgtggt gatccacacc
     3541 tcgacgtcgt gcgtcatcgg cagcggcgtg atcctgggcc atctggcgat ggtgcacgga
     3601 gcgcgcatcg acgatgcgac ggtcatcggc atgcaggcca cgctctgcaa tgagtctgtc
     3661 gtcgggagct ggacgatcgt cgccgagcag tcgctggtcg tgaagggcca gacggtcccc
     3721 ggcggggcca ttgtcggcgg atcgcccgcg cgggagatcg gcgcactcac ggagcgccat
     3781 cgcgcctaca tcgtgcacgc ccggcgcgtc tatgcggagc tcgcgcgcgg gttcgcggag
     3841 gggctgcggg ttgtgggtta aaaaacggcc gggttatccg gggcgcgatc atctcccgcg
     3901 gagcggtgtt tcgcattcaa acggcgcggg gcgtcgtcac cgtgccgcgg gacagcgtgc
     3961 accgagcgga gtagccgccg cggtcccgcc ggcaggactc ctccccggcg cccttcaatc
     4021 ggcacacctt tttatctttt tttattgcgg aaaaccgcgc gatgatcggg aattgtaacc
     4081 atcccacgca caaacggaga aatcaatgag aatgcgcata tttgctgttc tggtcctttc
     4141 actggcggtg gcggtatcgg cgtgctccaa gtcgcccgat cccggcgagg tgatcaccct
     4201 ctcgtacagt attttctttc ccgccacgca cgagcagtgc atcgccggca tgaactgggc
     4261 caaggaaatc gagaaaaaga ccgacgggaa ggtcaaaatc aacgtctatc ccggcggcac
     4321 tctcaccaag gccgacgaaa cctataacgg cgtgagcgcc ggcatcgccg acatcgggat
     4381 gtcgtgtttc gcctacaccc gcggccgctt cccgatcatg gaggccgtcg accttccgct
     4441 cggctatccc gacgggaaga ccgcgaccgc ggccgccaat gagttcttca aggccatgaa
     4501 acccaaggag ctcgacgacg tcaaggtcct ctatctccac gcgcacggtc cgggactcct
     4561 ccatacgaaa aagcccgtga agaagctgga agacctcaag gggatgaaga tccgttccac
     4621 cggcctcagc gcgaaggtcg tttccgcgct cggcggcgtt cccgtcgcca tgccgcaggg
     4681 cgccacctat gaatcgctcc agaagggcgt cgtggacggc accttcgcgc ccatcgagac
     4741 cctgaagggc tggaagcagg gcgaggtcat caagcacacc accaacagcc ggaccatcgg
     4801 ctacaccacc ggcatgtacg tgatcatgaa caaggcgaag tgggacaagc tccccgacgc
     4861 ggtcaaggcg gtctttaccg acgtgagcga tcgctgggtg aaggtccacg gcgagacctg
     4921 ggaccgcgtc gacggcgacg gggtcgcctt cacgaagaac ctcggcaacg gcatcatcga
     4981 gctcgacgcg gcggagggcg cccgctggag cgcggcggtc aagccggtca tcgacggcta
     5041 tatcgccgaa atgaagaaga agaaccttcc cggcgacaag gcgatcgccg agctacggag
     5101 gttgatagcg caatccggca agtagggccg ccggcggcgg gaatcccccg ccgctttcgg
     5161 acgccgatcg cgcgagcgcc gatgaacaag ctcgagtact atctgacgcg gatctccgac
     5221 tcctataacg tggtcgcggc ggcggccgtg gtgctgatga tgctcgtcat catcgtcgat
     5281 gtcgtcctgc gcatcgtgcg ggtgtcggtc cccggcgcct acgatctcat cagcctgctg
     5341 ggcgccgtgg cgatcgcctt ctcgctcacc tatacctcga tccagaaggg gcacatcgcg
     5401 gtcgacttcc tctatcagaa acttcccgag cgctggcagg ccggtttcga cgtgttcaac
     5461 gagtcggtgg gcgccggctt cttcgccgtc ctcgcctggc agtgcttcgc gtacgcggcc
     5521 tcgctcaggg ccgcgggcga ggtgtcgctg acggtgaagc tccccgtcta tccgtttgtc
     5581 gcgggcatcg gggcgagctg cgcgctgttg tcgctctacc tgctgctcaa tctcgccaag
     5641 gcgatccgga ggatggtgaa atgatgacgc caacgaccgt gggcctcgtg ggcctgttcg
     5701 cgctggtcgc gctcctgttc acgcggatgc cggtgggctt catcatggcc gtcgtgggat
     5761 tcgtggggtt cgcggccatc gtttcgttcg acgcggcgct caagctcgtc gcgaacgacg
     5821 tctactcggt cttctcctcg tacaacctca cggtgatccc gctgttcgtc ttcatggggc
     5881 agctcgcgtt tcattcgggc atcagcacgc ggctcttcga cgcggcctac aagtgcaccg
     5941 ggcgccttcc cggcggcctc gcgatcgcga ccatcggcgc ctgcgcgggc ttctcggcca
     6001 tctgcggatc gaccaacgcc accgcggcca ccatggcggc cgccacgttg cccgagatga
     6061 agcgctacaa ctacaagccg ggactggcga ccggggtcgt ggccgccggc gggagcctgg
     6121 ggatcctcat cccgcccagc gtggtgttca tcgtctacgg aatcctcacg gagcagtcca
     6181 tcggcaagct gttcatcgcg gggatcatcc ccggaattct gcttacgctc ttttttatcg
     6241 ccgccgtgct tatctggacc acgctctatc ccgaccaggg accgcccggc ccgcgcacgt
     6301 cgctcaagga gaagctggtg tcgctcgcgg ggctcggcga ggcgctggtg ctgttttcga
     6361 tggtcatggg cgggctcttc gccggcatct tcaccccgac cgaggccggc ggcgtggggg
     6421 ccttcggcac cctggcgatc tccctggcgc gccggaagct ggactggaag gggttcatgg
     6481 cggcgctcaa cgagaccacg cgcatctcct gcatgatcat ggtcatcgtc gcgggcgcga
     6541 cgatcttcgg ccacttcctc gccgttacgc gcattccgtt cgacatcgcg cagtggatat
     6601 cgggcttcga catgcaccgc gccgtcatca tgctcatgat catcgcggtc tatgtgatcg
     6661 gggggtgctt tatcgacgcg ctcgcgctca tcatgctcac catccccatc ttctatcccg
     6721 tggtgctgca cctggggtat gaccccatct ggttcggggt ggtcatcgtc atggtcacgc
     6781 agatcggggt gattacgccg ccggtgggga tcaacgtcta cgtggtcagc ggcgttgcgc
     6841 gcgacgtccc gctcgaggtc atcttcaagg gcgtggtgcc gctgctcgtc gcgctcatcc
     6901 tcgcgaccct gctcctgatc cccttcccgc agatcgcgct gtttttgccg gggttcgtga
     6961 agtaatcgat cgacgcccgg gagccgcgcc ccggggccgc gcgcctagta cttcatgtag
     7021 tggaagggct ccaccgcatt gagcgcgtcg gcgtccagcg tcttgtagcc ctcggcggcg
     7081 agcatcctca gcgcctcctc gatcttctgc acgtcgatca cgaacacggc cttctgccgg
     7141 ctctcgagca cgaacccgta cgcgttctcc acgttcacgt tcttttcgaa caagcgctgg
     7201 atcagccgat cgaggctgcc cggcgcgtcg tcgatgagga ccgcgatcac ctccttgagc
     7261 tggtttgcga tgccggcctt ctcgagcgcg gcgtgcgcca gtttcgggtc gtcgacgacg
     7321 aggtggaaaa acccgctccc gccgaacgag gtgatggtga tcgcgcggat gttgatcttc
     7381 tccttcgaaa ggacggaggt cacccggaga agcgtcccgg gcttgttctc ggccggtatg
     7441 gtgatttgaa atgcggtcat agtgtcctcg tggcgccgcc gcgttccggc ggctcgggtg
     7501 gtgtgctcgg cgcgtgcgcg gatcatacac gccgccgttt tgaaaaaaag tcaagcccat
     7561 aacgggcgat gcggggcagg ggtgatgccg cgcggttact ccttgagcgc ctcgtgcagc
     7621 acgatcgcgg cggccaccga cgcattgagc gaggcgacca ttccgcgcag ggggatggcc
     7681 acgatgtggt cgcagcgctc ctcggtgagg cgccgcatcc cctcgccctc gctgccgatc
     7741 acgatcacga ggggccgcag tccggggagc tcgtcgaggc cgatcgttcc gtcggcggcg
     7801 gttccgacga tggagaaccc gcgctcgcgc gcaagctcga tgaagtgcgc cgcgttgggc
     7861 gtggtgatga cgcgcaggtg cgccgtcgcc ccggccgagg ccttgaccac cgtcgcgttc
     7921 acctccgccg ttccgtgccg ggtaagcgcg accccgtcgc cgccgagggc ctccacggtg
     7981 cggatgatgg cgcccacgtt atgcgggtcg gtgaggtggt ccaggagcac gagcacgccg
     8041 ccgcgcgcgg acacctcggc gacgaactcc gcgtcggtga cggtctcgcg ctttcgccgc
     8101 gagaccagca tcacgccctg gtgcgtcgat gacgggtgcc gcgcgaaatg cgccttctcg
     8161 acgagctccg cgcgcgcgcc gatgcgtttc gccgccgcga cgatctcgtc gatgatcttc
     8221 ccgtgggcgc tcttcgagac aaagagctcg atccccgcgc ccgtatcgag cgatcggagg
     8281 tattcgagga cgggctttct tccgaggatt tccatggcct atttcttcac cttccagcgc
     8341 accccgtcct tggtgtcctc gagtatgatc ccttcggcca gcagctcgtc gcggatcagg
     8401 tcggcgcgcg cgaagtcctt cgccttcttc gctgcggcgc gctcggcgat gcgcgactcg
     8461 atccgtgcgg cgtcgactcc ggcgtcggcc tgcgcgggga agaagatgaa gccgagaacg
     8521 gcgtcgagcg cgcggagcgc cgtgatgacc gcatcgcgtc cggccgccgt catcgtcccg
     8581 cggtcgatga gcgcgttcac ctcgtgcagg aagtcgaaga agtgccccat gccgccggag
     8641 atgttgaggt cgtcgtccac cgtcgcggcg aacttcgcga ggaacgaatc gatcgcgccc
     8701 gagacctcgg cggcgaccgg cccgtcgccc ttcgcgtccc cgaggcgcac gatgaggttg
     8761 tcgatgcggt ccagcgcggc gtcggcctgc cggagaccgt cgaaggtgaa gttgagctgc
     8821 ttgcggtagt gcgctgagat gagcaggtag cggatcgagc ggggcgagtg gcccttctcc
     8881 agcaggtcgc gcagcgtgta gaagttcccc agcgacttgg acatcttttt cccctcgacc
     8941 agaaggtgct ccacgtggat ccagtgccgc acgaacggct cgccgtaggc gcactcgctc
     9001 tgggcgatct cgttctcgtg gtgggggaag atgaggtcaa cgccgccggt gtggatgtcg
     9061 atggtgccgc cgaagatctt gcgcaccatc gcggagcact cgatgtgcca acccggtcgt
     9121 cccgggccga agggcgtgtc ccacgacggt tcattcggcg cggtcgcctt ccagagcgcg
     9181 aagtcgcgca cgtcgtcctt ctcgtactcg tcggtgtcgt agcgcaggcc cgacttgatc
     9241 tcgcgcgtgt cgagtcgcga gagccgtccg tagcgctcgt acttggcgat gctgaaatac
     9301 acggaaccgt ccttctcgta cacgaaaccc cgctcgcgca gccgctccat gatgtcggcc
     9361 atcgcgtcga tcgactcggt cgcgcgcggc atgtgctcca ccggctcgat gttgagcgcg
     9421 cgcaggtcct cgaggaagat gttcgtgtat ttctccgtgt actcgcgcag ggtgatcctg
     9481 tccttctgcg cgcccgcgat cgtcttgtcg tccacgtcgg tgatgttcat ggcgtggttc
     9541 accgcgtagc cgcggaagcg cagataacgc cgcaggaaat cgttgaacat gaaggtgcgg
     9601 aaatttccga tgtgcgcgta accgtacacg gtggggccgc aggagtagat ggatacgggc
     9661 gggtagtcct cgacgcggtc ctttcgcgcg ccgccgggca cgatctcgtc gggaagtccg
     9721 gatatggtgt tgtgtacgat gatgggcacg ggtaacctcg ctggtgaaaa gatggtcagc
     9781 gggtcgtccg gccgtcgggt tcgagttcga tttcgtgtcc cttcatgccg ttgtagagcg
     9841 ggtaggggac gaaggagcgg acgcggcggc ccagcatgac ccgcttgtcg ccgtaccaga
     9901 ggaagatcca cggcgcgtcg ctcacgatca actgttccgc gcggcggtgg atgtcaattc
     9961 tttttcccgc gtccatcgtg cgccgcgcct cgtccagcag gcggtccacg gaaacgttcc
    10021 gatagaaggc gcggttcccg ccctggccga tgttcttcga ataaaagagc gggtacagga
    10081 aattctccgc gtcggggtag tcggcgtgcc aggtgaagta gcacaggtcg taggcgccgt
    10141 tgagcgtcgc cgccttgagc gccgaccatt ccatctctcg gatggagacc gcgagccccg
    10201 cggcggcgag atcgcgctgg accatctgcg cgatcagcga cacctggtgg tccgatttaa
    10261 tgaagagcgt gatccggtgt ccggaaagtc cgagcgaagc gatcgcggcg cgcgcggcga
    10321 cgggatcgta gtcgtacggc cgcgccgcgc tccggtggcc ggccagcgta ttcggcaccg
    10381 ggcccgcggc gagcgtgaag cggttgtcga acagcgcccg catgatccgc cgccggtcca
    10441 cggcgcggtt gagcgcggcg cgcagggccg ggttgtccag cggcggccgg gcattgttga
    10501 tggcgatgta gtggacgctg tattccggca cgtcgatcgt ccgcgcctgc gcgtcgtggc
    10561 gtacgcgcgc gagatacgga acctcgaagt agtccagtgt cccgttcagg aactcgaagc
    10621 gcgccgtgag gtcctcgggg atgatgcggt agacgatgcc ggagaccctc gccggtccct
    10681 cgaagtagcg ctcatggcgc gcaagcacga ggcgctcgtc ctgccgccac tcgcgcagcg
    10741 cgaagggccc ggtccccacg acggacccgt cccggcggtg cgccgcggcc gagatgatat
    10801 aggccgtcgg ggtggccaag aggtgcggga acgaggccga cggctcgcgg agggtgatca
    10861 cgatccgccg ctcgtcgggc gtcgcgatgc gcgcgatgtt cgcgaacagc cagcgccgcg
    10921 gcgaggtcgg gttctccgag aggcggacgc ggtccagcga gtaggccacg tcggccgcgg
    10981 tgagggcggt cccgtcgtgg aagcgcaccc cgtcgtgcag cgtggcctcg agccgcgtcc
    11041 cgtcgaaccg gtgcgtcgca gcgaggtcgc gctccaggga gccgttggac gaaatcttga
    11101 aaagcccgtt gaacaggtgc gccatcaccc gcccggacac cacgtcggtg ctgtagaagg
    11161 ggtccagtcg ggcgggatcg gactggatgt aggtgttgaa atggtccgtc gggcgctcgc
    11221 cccggcaggc ggccgcgcag gcgagcgcga tgcagcagag gcgcgcgatg cgcacgaggc
    11281 gggcggcgct acgcttcggg ctcgttgtca ccgaggacga cctcctcgac ctcgacgaac
    11341 tcctcggcgg gaatctccgc cacgcgctcg atcgcaacga ccggcacggg ttccggctca
    11401 accggggtca gcgcgggaag atcgatgtcc cgcatgcgct ccgggtcctg gcgataggcg
    11461 gcgatcagca ggtgcagctt cttgatgtac tcgatgagct ccttgagctt ccggttcttt
    11521 ttgtcgatgg tctctttcga gcccatgagc agccgcttga tgagctcgtt tttcttcttg
    11581 agcttggtct gtagctcgcg cacgcgctcg tagtactcct tgttcacctt ctggaagtcg
    11641 atgcgcgcga tgacgatatc gccctcgtcc ttgtccttct cgatgctctc gaggatcttg
    11701 ttgatttcct tgatggagaa caggtcgtcg gccgaggtct gcacccactt ggtcccctcg
    11761 gtgtcggtga actccgtgaa gtcggggacg atggtcggtt tcttggggcg ctttatcatc
    11821 aggctcatct ggtgccgata ccagcttcca tcataaagat tgaacgcacg gtgaatggat
    11881 agtgtcgtct gtcacacgca cgcatgacat ggcagtatac tcactgtagc taattatcgg
    11941 cacaatcatc attaatatgc agatatatcc cgcggcaagc aaaaattgcc cggcatgggc
    12001 gttgatatgg gattgacacg ccgaaaagcg atacctatcg tgccggttgg ttgtaaatac
    12061 tagaaaagag tgatgataga tccggacatt ttgaaggagc ttgcgccgtt cgggcgcgtt
    12121 cgcgaatacg cgcccctgcg cgagtacacc accttccgcg cgggcggacc ggccgacgcg
    12181 ctcgtggttc cgaataccgc cgccgatgtt ccaggcatcg tccgcgtcgt gcgcgagcgc
    12241 gcgctcccgc tcacggtaat cgggggcgga tcgaacctcc tcgtgggcga ccgcggggtc
    12301 cgcggcgtgg tcctcgcgat ccgcgagcgg gacgaagccg ccggcgcaat cgccgtttcc
    12361 gacaatggcg acatcgcggc ggaggcctcc gtgagcaagg agcgcttcat cgacttcgcg
    12421 atcgcgaacg gcttcggcgg tgtcgaattc atggcgggca tccccggctg cctcggcggc
    12481 ggcatcatca tgaatgccgg gaccacgctc ggctggtttg ccgacattct cgtcgggata
    12541 cggattgtcg acggcgccgg cgcgctgcgc gatctacccg tcacgaaaga aatgtcgacc
    12601 taccgccgcc tgcggatcga cgatgattcg attattatcg cgggcgcctt ccgactgccg
    12661 cgcgccgagg atcccgcatc ggtcagggag acgatcgccg ggctcctccg cgagcgtgcg
    12721 gggaagcacc cgctcgacta tccctcggcg ggatcggtct tcaagaaccc cgagggtcac
    12781 tcgtcgtgga agctcatcaa cgacgccggc ctcaagggca ctcggatcgg cggggcgatg
    12841 gtctccgagc tccacaccaa cttcatcatc aattacgaca atgcgaccgc gcgcgacatc
    12901 cgcgatctca tcgagcacgt ccgcgaaacg gtgcaggcga agttccaggt gcgcctggag
    12961 accgaggtgc ggatgctggg cgagttctag ccgtcaggtt tttttcagca ctgattgcaa
    13021 cgaccccctc ccggcctccc ccttcgttag ggggaggcgc gcaactggtg tgtgacttta
    13081 cctcgctccc ctttcccctg atgaatggga aaggctccca acaggaggtt gcagtgccgc
    13141 acaggatgtg caaaaaggcg acggcgccga aaagcgctcg gcggtcgggg gataggggta
    13201 taatcacttt tgtttgcggc ttggctatga tttgcgtgat ccggaagcgc ttggcggcat
    13261 cgacttttta tcagccacgc gataaaaagt cgatatggct gaacgatagc tccacatggc
    13321 gtttcaggtc gtcgagcaga acatcgacag ggttgtccgt tcatcaaaca tccgcttcat
    13381 ggacacggaa aagtttctct cgatgttcgt gtgaccggca gccgccctac aacagaatca
    13441 ccacccccgc cgccgcataa ttgaactgtg agaacgtgta cacggtctta ttgtccgcgc
    13501 cgccctcgag gatgcggtag ccgagcttca cgatcacgtt cttgctcgcc tgatacagga
    13561 accccgcgaa gacgtcctca gcgcgcccgt agggcgaggc gagggcgtcg ccgttcacaa
    13621 gcaggctgaa cggccccgcg aatttccatt cgatgttgaa attgaggatg ggcacgaacc
    13681 ccgtgttctt ctcctccgcg atgaggtcgg ccgattccat cttgatggat gcgtcgcgta
    13741 tcttcgcggt gaagcccgcg ccgatgcgcc acgattcgtt cgtgaagaag tcgtagcgat
    13801 aggtgacccg gtaggaatcg aaccggaagg tcgtctccac gtactcgccc tcgcggaagg
    13861 tcgtgtagcc gaagcgcacg tccttccgaa gcgtgccgag cgataccaca cggagcggcg
    13921 cggcgaggaa cgacaccgtg tggcggtccg aaaaggtgta cccggcccgt ccgcggtaga
    13981 agtacgtggg ctgcgcctcg agatcctgcg tgagcgagaa cctcgtcccg cccttcgccg
    14041 ggatgcggat atcattgtag ccggtgtcca cgtaaccgcc ctcgaggtcg atgtggtatt
    14101 gcgccagggc cggggtggag tatgccgcaa ggcttattga tgcaaatgct attgcaacat
    14161 ggagaatttt catggtaatg cctcatttgt aagttcttga taattgaaaa tagcaccccg
    14221 atggaggtgc gcaagaaaaa cgagaggatg gtaggcgaaa ttcagcgaaa gagttccgat
    14281 gtgattatcg atatccgaaa gcaaccgcgc gaagcggcgt ggtgattccg gttcccgaaa
    14341 gttgttgttc gttccaggta ttgccgccgt cggtggaaag gcaaacagcc gcccaggcgt
    14401 tgaatcccac agctacccat tttccctcgc cataggctat tccccacagg gtgggagttg
    14461 tcagcgtttt cggggtccag ttcgcgccgt cgtcgtcggt gaagtaaatg actggattcg
    14521 gatgggctgc ggccatgacg cgctgtttcc cgtcatgtga aaggtggtag atgtttgagg
    14581 ttatgatgcc tgcggcttgt ggcacccacg tatcggcgtc cgtcgaatag aaataggtga
    14641 cggtcggtcc gcaggcaacg aagcgactgt tgccccgtat caaaccgtcc gaggcgattg
    14701 ctggagcgga ggacgcggga gtgaaggagg cgccatcgtt ggttgaaaaa cgatatgcgg
    14761 ttcctcccga cccaacggtt gcgaacatcc cattggcata ggtgatgttg tagatgtcaa
    14821 agctgctgta ttcggtccac gttgcagcat tatcggtcga gcgagcgtaa aaattttgcc
    14881 ccacggcgat ccaggcgccg gatctgcttg tcgcgacgtc cctgaagacc gcacaggtcg
    14941 gagcggtaaa acgaagcatc cacgctgcgc ctgcgtcgtc ggaagtgtat accacg
//
LOCUS       DAORHF010000072        14920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3073_length_14920_cov_4.936428, whole genome shotgun sequence.
ACCESSION   DAORHF010000072 DAORHF010000000
VERSION     DAORHF010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14920
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3073_length_14920_cov_4.936428"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PKJ16_07930"
     CDS             <1..194
                     /locus_tag="PKJ16_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46954.1"
                     /translation="SDSAFELNQLKEFNDRLILFFDRAQYYVIRSYMAELSATLQQAC
                     KVSADDAEKLFFTKSFYNK"
     gene            complement(197..1129)
                     /locus_tag="PKJ16_07935"
     CDS             complement(197..1129)
                     /locus_tag="PKJ16_07935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV46955.1"
                     /translation="MSVTEPGAREGGIAPKPRVLVVDDDPGNRYLLGNVLDDFEVEVA
                     VDGAEMMERIAHAVPSILLLDVMLPGRDGFQLAAELAGDDRYRNIPVIFLTARTDPAD
                     VVTGLELGGYDYIKKPFDEAELVARIRSALKRSTERQRLAHDVIIDPHTGLYNRRYLS
                     DFIKNESGKIKRSLSDFAIAMVDIDHFKRLNDTYGHQCGDYVLKEFAHVMRTCVRTYD
                     LAIRYGGEEFLVVLPAASRNDALQVLERLRAANAAHEYRYGSIAVSFTFTCGIADLAD
                     IDPARSVFDDLIRVADERLYRGKRNGRDRIVAVD"
     gene            complement(1126..1518)
                     /locus_tag="PKJ16_07940"
     CDS             complement(1126..1518)
                     /locus_tag="PKJ16_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV46956.1"
                     /translation="MKHSILIVEDAYPNRFVLEQLLEEYECQFAVDGSQMWEKLSAHI
                     PDLIIMDIGLPREDGLILAQKLQKDDRYRGIPVIFLTAHSSKKEIVTGMLAGAYDYLV
                     KPIDGTILLERIDAVLKRKVREQHGGAR"
     gene            complement(1515..4775)
                     /locus_tag="PKJ16_07945"
     CDS             complement(1515..4775)
                     /locus_tag="PKJ16_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46957.1"
                     /translation="MEPIVTDLICRFTPDFTVIFANKAFRARYAMGMAPGSVPHLPVG
                     DRDVLAGLTPLEPSTTCRREYHAEDGASGWLEWIHHASFDSAGRPVRYQSIGKDVTEL
                     VRLETILTIQRDLAIALHGPYTVSESFDIVLQALMKIPPIDSGGIYTLDEKNDSIRLV
                     SHRGLSEAFIDEVSEFKSGTSQYRIIMDGSPVYREYADLAGDATPLRRTEGIRAIGVI
                     PVQHEGRVIAALNIASHESNLFPVSVRNAIETIVAQIGNILHEIRYREQIFIQERNLV
                     SLFNSIDDCVFVLDAAGVIVEVNSAVLKTLGYAREELVGRDILLVHPPDRRDEARDVI
                     ASMLRKDASTCRIPLCEKDGTLVPVETVVAHGSWNGVPAIFGISRNLSDRMNLQRKLD
                     QTNERYALALQGGNLGAWDWNIPTGDVVFDERWTAMLGYELDEIEPTLASWERLLHPD
                     DRPQVEEALRDHLSGRTPIYQTVQRLRTKSGEWRWILDTGKVVEWDDAGAPLRAAGTH
                     LDITERIESSLSLEFRERLEGVIMTLSTDFINLDSEEIDAGIDRSMRAIGTLLNADRA
                     YVFLFSDDGSRMSNTHEWCAPGVVSQRDSLQDLVAGDFPWSIGRLRGWETVAVRDVHE
                     LPPEAAAERESFERQGIMSLVLVPIIHGDELMGFAGFDSVAEHRIWEREAISLIRTVG
                     ELIAVVLKRKRYEREIRESRAEAERANHAKSEFLANMSHEIRTPMNAVIGISKLLMEK
                     NAGNLTAKQLEGLRLVYESGVRLLAIIDDLLDLSKVEAGKAVVRPVVFSLQGLVDEIV
                     ALTRTLVADKPVRVVNAIDGAADDIIADSDKVRQILVNLVGNAAKYTDSGEIRIAGAV
                     RDGILECSVSDTGIGIEEGFLPHAFEPFTQADISPSKRYAGTGLGLALCKRYSDLMGG
                     DISIASRPGAGTTVSFRIPVSRPGDHIVSSDGAATPVRGGAPVRDRPHILIIDDDASA
                     RATLRLMLEDAYEIEVASNGSEGVGRFFARRPDAVLLDIMMPEMNGFEVFDRIRSGTK
                     GGPTVPILAVTARAMAHEIARILAHGFTECITKPVDNTLLHATLSRVLAKAPESAT"
     gene            5075..7243
                     /locus_tag="PKJ16_07950"
     CDS             5075..7243
                     /locus_tag="PKJ16_07950"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV46958.1"
                     /translation="MKLTNRDHIVSGNGRTAVVKRNEHGIPEISGDSPADLAFGQGWV
                     HACDRQLQALLAKTLTAGRAAEQLAAEPALIEIDRYMRRLNLLPDADAQIKKLEPAVH
                     EQLQAYTDGFNAYLTAHGVVFELRLLGYRAEPWEIKDTLRLGKAMGLIGLADAQGAME
                     KFIVQMIQHGIDERRLKELFPYLTERIDATLIGKIRLSPPLAPESLAWLSAVPRLIAS
                     NNWVIRGARSESGKPLLCSDPHLEVNRLPSIWQELVMRLPDDTIIGSSIPGAPGLPIG
                     RTRRLAWGATYSFMDQIDFRIEECRDGAYRRGSSWKPFTVRREEIRLKKGRPVIETVY
                     ENELGPLEGDPNVAGHYLVMCWSAARGCIAKDFNGILGMTTAKSVKEGMALYRRLEAN
                     SFNYVLADDSGNIGYQMSGRLFNRPRGASGLVPLPAWDTKYNSAGFVPAAKLPSQYNP
                     ASGTIVTANQDLNRFGKAKPINLPMGSYRADRIEQLLKKKSKYSVEDMKAIHYDLYSL
                     QAEAFMKHLRPLLPDTENGRALRDWDCAYTADSTGAMLFESVYHALLRVVFGDGGMGR
                     EVVEHLMAETSLFNDYYANFDRILLGKRSAWFNDRPKDELFRRAIDEGLSVPAAPYGK
                     TRKITLAHLLFGGKLPSFLGFDYGPIELPGCRATIPQGQIFRSAGRTTTFSPSYRMIA
                     DMATRELHTNLAGGASDRRFSKWYVNDMKNWLGGVYKLLR"
     gene            complement(7255..7328)
                     /locus_tag="PKJ16_07955"
     tRNA            complement(7255..7328)
                     /locus_tag="PKJ16_07955"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7292..7294),aa:Arg,seq:tcg)
     gene            complement(7372..8772)
                     /locus_tag="PKJ16_07960"
     CDS             complement(7372..8772)
                     /locus_tag="PKJ16_07960"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV46959.1"
                     /translation="MKVLLVIPPATERRRVYSLPSLGIAHIAAVLMARHEVMVLDYAV
                     HGFSERRFDRAVRSYQPDCVGFGCTSPMFAYAVGLARIVREAAPRAKIVFGGPHVTAL
                     PDASVAHGEIDCVVMGEGEYALAELCDVLDGGGSPAGVAGIAWKDDGEVRVNETRAPI
                     ADLDALPFPAWGLLDMPRYYSRFRPSASMITARGCPFACTFCASRLVHGTRVRRRSVE
                     HVMEEIELLANRHGVRYLYFFDDTFTLDRDHTLALCERLASMRARLSYWCYTRSDCMD
                     PELARALRRSGCESIGIGFESGSPEVLMAMNKRCTIEEGRAAAEAVRAAGIFLEGFFV
                     ANHPGETMADFGKTASFIRSLDLSFYDINFAVAYPGTALADREGSHGCSDWFTEATAS
                     RMSSEERKIAAEVRSLRRRVFTRASFYRLLWRTVRCGSYGAAFIRHTGRVLMTMVSRL
                     ARPSGSDARLRSDNTS"
     gene            8856..9887
                     /locus_tag="PKJ16_07965"
     CDS             8856..9887
                     /locus_tag="PKJ16_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46960.1"
                     /translation="MSAQPKALRIGGIVIAILAIIAVVALCLRPLVRRHRLSPVVTSA
                     IEKGVGYLERSQLPSGEFRTCASADPLMRDECAFDGSPFATSLIVYSLSRASHPAIGG
                     ITERALRFLRDDMSGPGIWRYWSRSNPKHRDIDPDLDDIACVSFVLRKFGAPFPPNER
                     LLIDNRAGEGRFRVWLRSGRFQVNEFDCVVDTNVLLYLGERPETQAACASVNAIINAG
                     TRCGRYYPSPLSLYYMHSRALREGIACLGKSREAAVDGVIRTVRGRESSGNALHAALA
                     ACALLNYQRENRTLDRLIDALIELQRPDGSWRRSTFYLGPAPYYGSDELTTALALEAL
                     AGYLKTIQR"
     gene            9984..12269
                     /locus_tag="PKJ16_07970"
     CDS             9984..12269
                     /locus_tag="PKJ16_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HNV46961.1"
                     /translation="MHEHETKQNRGSAIVSRFRALLDDLYIGEKTYSRVSGWLITMVR
                     ASIATTRKFIEDDCPTMASSIAYTAIVSLIPTLTVALTFYSLLAGMGAKKEELFRQIM
                     VFMTEHNIKLNIDPMIEAITALIDNAGKIGGIGAVILIFSATAVLRTLEKSLNTIWGV
                     QQNRPIMLRVIYYWAALTLGPIMLITGTTVATQVSNALSSPNFHAAAITETGAIWTAG
                     NKARILSSKGDLAFTRIDRRSVDFENSRVMEYDTASRNFYQQEYRIEEFDFDRGSFRD
                     VQFMGSRGWIVGAKGMLLTTEDGGRHWALARWGSYNFNAIHMLTPMRGFIAADNGFLL
                     STTDGGNSWAVRDWVDITANLFAIDFRGQRGIVVGERGAFIATTDGGATWDAGKLNES
                     RVMRRHVNLNHVRFANGSVWIVGDEGLILRSTDGGTTWKSRKHLDINYFTAYFIDARR
                     GYVAGEKGALLSTDSGGERWTRHSLATPRVNRIQLANGTLWAFGHNGFIQRSTDGGQR
                     WSGLRGSGFFIGILNFLAPFVIIWLMFFLVYLVLPHAKVPARAALIGSSFTGAVWVAF
                     TLLFIVYVKYFSYGTHAIYGALASIPLFLLMIYSSAVIFLYGAQVCYTAMHPHTYLKL
                     KKKSATERSMSVYNGISLLHAVYRRFESGGGAMSLRELQKATGQGPEELERYLRIFSK
                     ARLLRYDADTEIAPLNASSNVTIASVIGLLHDSGLTPPASAAGPAHLRKYLSEVLADI
                     AKSRAKIVGNTTLKDLIAKAP"
     gene            complement(12273..13154)
                     /locus_tag="PKJ16_07975"
     CDS             complement(12273..13154)
                     /locus_tag="PKJ16_07975"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HNV46962.1"
                     /translation="MTRRSDINLSLVLLMSFFLHTVMMLGIVIPNYYAMLQDQMNRDR
                     TRKMFGGRDIIVNINADRQPVISPKTLLSDEDSSAKGHLTKEKGDHWLNNSRDFAQKR
                     GGKKSGNSSTVQTKSSQIPKRMLLNDESELVVEFMKLSEGGYTLFGDEGGEDFTKVPD
                     RNTFSKNNAIFYSNDGRFSFNTKRFRNFRYFKQMKDKIAANWHPPDMAKSVIGGYAPG
                     YVKINAIPDQVVKLYFTMDRAGNVLDVVIVESLGNRQLDQSCVESIRWSKSFGPVPED
                     VKGQIVVIPFMFGYFIY"
     gene            complement(13151..14440)
                     /locus_tag="PKJ16_07980"
     CDS             complement(13151..14440)
                     /locus_tag="PKJ16_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HNV46963.1"
                     /translation="MNVVVFEDGLVDQFYPLSLTRPVWELRCGCFTGRERIQRFLDSE
                     GGSARSASTVVFLTRPHLAAYYRERYPDIVINDCSRLRSGVDVLFINARYVDCGAALD
                     LQAGYGIARQGAPLAVRLNPTAADAVVSGLSEHSADSIEKMLVSSGVALREADGETAV
                     DYIWDIIEMNPAQIARDARFYTKRTPNPLPPQVTIIGDGSLLLIGDDVRIDPFVCFDC
                     TAGPIIVGDGCVINSFTRIEGPCFIGGKAVLLGAKVREGCSIGPGCRIGGEVEESVFQ
                     ANSNKYHDGFIGHSYIGEWVNLGALTTNSDLKNNYSSVKCYVPDRRVNTEKLKVGCFI
                     GDFSKASIGTLINTGTSIGVGCMLVHAGAMPPSHVPSFAWFFENELHDRGSLPGFLKA
                     AAAMTSRRGIACTPAFTGMLEAVHDITAPQRRGMGHR"
     gene            complement(14455..>14920)
                     /locus_tag="PKJ16_07985"
     CDS             complement(14455..>14920)
                     /locus_tag="PKJ16_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV46964.1"
                     /translation="GVLARFPVVDVAVEVFDGSYHPVDSSEMSFKIASRGAFKKAMEG
                     AAPQLLEPIMEVDVYVDKDYMGDILNDITSRRGRVLGMGSTDESKGGGVSVVKATVPL
                     AEMLRYSIDLRAKTSGKATFEMKFSHYDPISGKIAEKVIEDRKKELEEEAAK"
BASE COUNT         2912 a         4821 c         4451 g         2736 t
ORIGIN      
        1 cttcggattc ggcgttcgag ctcaatcaac tcaaggagtt caacgaccgg ctgatcctct
       61 ttttcgaccg cgcgcagtat tacgtcatcc gatcgtatat ggccgagctg agcgcgacgc
      121 ttcagcaggc gtgcaaggtc tcggcggacg acgccgaaaa gctgtttttc accaagtcgt
      181 tctacaacaa gtaacctcaa tcgacggcga caatcctatc gcggccgttt cgcttgccgc
      241 gatagagccg ctcgtcggcg acccgaatta aatcatcgaa caccgaccgc gccggatcga
      301 tatcagcgag gtcggcgata ccgcatgtga acgtaaacga taccgcaatc gatccatatc
      361 gatactcatg agcggcgttg gcggcgcgca accgttcaag aacctggagc gcgtcgttgc
      421 gcgatgcggc cggcagaacc accaggaact cctccccgcc atagcgtatc gcgaggtcgt
      481 acgtgcgaac gcatgtgcgc atgacgtgcg cgaattcctt cagcacataa tcgccgcact
      541 ggtgtccgta ggtatcgttg agccgcttga agtggtcgat gtcgaccatc gcgatcgcga
      601 aatcgctcaa actccgcttg atcttcccgg actcgttctt aatgaaatcg gaaaggtagc
      661 gtcggttata cagtcccgtg tgcggatcga tgatgacatc atgcgccaat cgctgccgct
      721 cggtcgatcg cttgagcgcc gagcgtattc gcgcgacgag ctcggcctca tcgaacggct
      781 tcttgatata atcgtacccg ccgagctcga gccccgttac gacgtccgcg gggtcggttc
      841 gcgccgtgag aaagatcacc gggatgttgc gataccggtc gtccccggcc aattcggcgg
      901 cgagctgaaa accgtcgcga ccgggaagca tcacatcgag caggagaatg gacggaacgg
      961 catgggcgat acgctccatc atctccgcgc catcgacggc cacctccacc tcgaaatcat
     1021 cgagcacgtt tcccagtaaa taacggtttc ccgggtcgtc atccacgacc agcacccggg
     1081 gttttggcgc tatgcccccc tcccgcgcac ccggctccgt gacgctcaac gggcccctcc
     1141 gtgttgctca cgcacctttc gcttgagcac ggcgtcgatg cgctcgagca gaatcgttcc
     1201 gtcaatcggc ttgaccaggt agtcatacgc tccggcgagc atgccggtga cgatctcctt
     1261 cttgctggag tgagcggtga gaaagatgac gggaatgccc cgatagcggt cgtctttctg
     1321 caatttctgc gcaaggatca aaccgtcctc gcggggcagg ccgatatcca tgatgataag
     1381 atcgggaatg tgcgctgaaa gcttttccca catctggctt ccgtccacgg cgaactggca
     1441 ttcatattcc tccagaagct gttcgagaac gaaccggttc ggataggcgt cttcgactat
     1501 gagaattgag tgtttcacgt tgctgactcc ggtgccttcg ccaggacacg cgatagtgtc
     1561 gcatggagca gcgtgttgtc aaccggtttt gtgatgcact cggtaaaccc gtgggccagg
     1621 atgcgcgcga tctcatgcgc catggcgcgc gccgtcacgg cgaggatcgg caccgtcggc
     1681 ccgcccttcg tccccgacct gatccgatcg aacacctcga aaccgttcat ttccggcatc
     1741 atgatatcaa ggagcacggc gtccggtcgc cgtgcgaaaa agcgaccgac gccctcgctg
     1801 ccgttgctcg ccacttcgat ctcgtacgca tcctcgagca tcaatcgcag cgtggcgcgc
     1861 gcgctggcgt catcatcgat gatgaggatg tgcggacggt cccgcaccgg cgcgccgccg
     1921 cggaccgggg ttgccgctcc atccgacgag acgatgtgat cgccgggtcg cgacacggga
     1981 attcgaaatg aaaccgtcgt tcccgcgccg ggacggcttg cgatggatat atcgcccccc
     2041 atgagatccg agtatcgctt gcacagggcc agccccagac cggttcccgc atagcgtttc
     2101 gacggcgaga tgtccgcctg cgtgaagggc tcgaaggcat gcgggagaaa gccctcctct
     2161 atgccgatcc ccgtatcgga gaccgaacac tcgaggatgc cgtcgcgcac cgctccggcg
     2221 atgcggatct cgcccgaatc ggtgtacttc gcggcgttgc cgaccaggtt cacgaggatt
     2281 tgccgcacct tgtcggaatc ggcgattatg tcgtccgcgg cgccgtcgat cgcgttcacg
     2341 acgcgaaccg gtttgtccgc tacaagcgtt ctcgtcaggg ccacgatctc atcgacaagc
     2401 ccctgcagcg aaaaaacgac cggacgcacg accgccttcc ccgcttcgac cttcgagagg
     2461 tccagcagat cgtctattat ggcaagcagg cgcaccccgc tctcgtagac gagacgcagg
     2521 ccctcgagct gtttcgcggt gagattgccg gcgtttttct ccatgagaag cttcgagatg
     2581 ccgataacgg cgttcatggg ggtccgtatc tcgtggctca tgtttgccag gaactccgac
     2641 ttcgcatgat tggcccgctc ggcctcggcg cgcgactctc ggatttcccg ctcgtatcgt
     2701 ttcctcttga ggacaacggc gataagctcg cccacggtcc ggatgaggct gatcgcctca
     2761 cgctcccata tgcgatgctc ggcaaccgaa tcgaagcccg caaaccccat cagctcgtca
     2821 ccgtgtatga tcgggacgag caccagcgac atgatcccct gccgctcgaa actttcgcgt
     2881 tcggcggcgg cttcgggcgg cagctcgtga acatcgcgaa cggcgacagt ctcccagccg
     2941 cgcaagcgcc cgatggacca tggaaaatcg cctgcgacga gatcctgcaa gctgtcgcgc
     3001 tgcgacacca cgccgggagc gcaccactcg tgcgtgttgc tcatgcggga cccgtcgtcg
     3061 ctgaagagga acacgtaggc ccggtctgca ttgaggagcg tgccgatggc gcgcatggaa
     3121 cgatcgatgc ccgcatcgat ctcctcggaa tcgagattga tgaagtccgt cgagagcgtc
     3181 atgatgacgc cttccagccg ctcgcggaac tcgaggctca gcgaggactc gatccgctcg
     3241 gtgatatcca gatgggtgcc ggccgcccgc agcggcgcac ccgcatcgtc ccactccacc
     3301 accttgccgg tgtcgagtat ccatcgccac tccccggact tcgttctcaa gcgctggacg
     3361 gtttgataga tgggcgtcct tcccgacaga tggtcgcgga gcgcctcctc gacctggggg
     3421 cggtcgtccg gatgcagcag acgctcccat gaggcgagcg tgggctcgat ctcgtccagc
     3481 tcatagccga gcatggcggt ccaccgctca tcgaagacga catcgccggt gggaatattc
     3541 caatcccagg cacccagatt tccaccctgc agcgcgaggg catagcgctc attggtctga
     3601 tcgagcttcc tctgaaggtt catccggtcc gaaagattac gcgatatgcc gaagatcgcc
     3661 ggcactccgt tccaggagcc atgcgcgacg accgtttcca cgggcaccag ggtcccgtct
     3721 ttttcgcaca gtggaatccg gcacgtcgat gcgtccttgc gcagcatcga ggcgatgaca
     3781 tcgcgagcct catcgcgtcg atccggcgga tgcacaagca ggatatcgcg ccccacgagc
     3841 tcctcgcgcg catagccgag cgttttcagc accgcggaat tcacctcgac gatcacgccc
     3901 gccgcatcga gcacgaacac gcaatcgtcg atggaattga acaacgacac gagattcctt
     3961 tcctgaatga atatctgctc acggtagcga atctcgtgca ggatatttcc aatctgcgcg
     4021 acgatcgtct caatggcgtt acgaacactg acgggaaaca ggttggactc gtgcgaggcg
     4081 atattgaggg cggcgatgac ccgtccctcg tgctggacgg gaatcacccc gatggcccgg
     4141 atgccctcag tccgccgcaa gggcgtagca tcgcccgcca ggtctgcata ttcgcgatac
     4201 acgggactgc catccatgat aatgcggtat tgggacgtgc cgcttttaaa ctcggaaacc
     4261 tcgtcgatga aggcctccga gagcccccga tgactgacca accgtatcga atcatttttc
     4321 tcgtcgaggg tgtagatgcc gccgctgtcg atcggcggga tcttcataag cgcctgaaga
     4381 acgatatcga acgactcgct caccgtgtac ggcccgtgca gggcgatcgc gagatcgcgc
     4441 tgaatcgtga ggatcgtctc aagacgaacc agctcggtaa catccttccc gatcgactgg
     4501 taccgcaccg gccgtccggc gctatcgaac gaggcgtggt gtatccattc caaccacccg
     4561 ctggcgccat cttcagcatg atactcacga cggcaggtgg tggacggttc gaggggcgtg
     4621 agaccggcaa ggacatcccg gtcgccgacc ggcaggtgcg gaacggaacc cggagccatt
     4681 cccatggcat accgcgcgcg aaacgcttta ttcgcgaaga taacggtaaa atccggggta
     4741 aaccgacaga tgagatcggt cacgatcggc tccattgctg attctcccgt catgaccgcc
     4801 ctgcggcacg aggggcggcc acgtattgct ccctgcgtag catatcgatg aagtcagttc
     4861 aacaaaaaaa catgaaagac cctgtcgcga gtgacatgac tgtttttccc agaaacgatg
     4921 ttttgtaatg gacagaggcg tttgctcctt atacaatact tctcacgaca atgaattgat
     4981 cgttcctcgg cgccgatttc cgccggggga tcgcatcaac gaccgatgcg catcttccgc
     5041 catcggcccg aagaacggca cgaggtggac gccaatgaag ctcacgaaca gggatcacat
     5101 cgtatccgga aacggccgaa ccgccgtcgt gaagcgcaac gagcacggca tcccggaaat
     5161 atccggcgac tccccggccg acctcgcctt cgggcaggga tgggttcacg catgcgaccg
     5221 ccagctccag gcgctgctcg ccaaaacgct gactgcaggt cgcgccgcgg agcagctcgc
     5281 cgccgagccg gccctgatcg aaatcgaccg atacatgcgg cgcttgaacc tcctccccga
     5341 cgccgacgcg cagatcaaaa aactcgagcc ggcggtgcat gagcagctcc aggcctatac
     5401 cgacgggttt aacgcatatc tcaccgcgca cggcgtcgtc ttcgagctgc gattgctcgg
     5461 ataccgcgcg gaaccgtggg agatcaagga caccctccgc ctcggcaagg cgatggggct
     5521 catcggcctc gcggacgcgc agggggccat ggaaaaattc atcgtgcaga tgatccagca
     5581 cggcatcgac gagcggcgtc tcaaggagct ctttccctat cttacggagc gcatcgacgc
     5641 aacactcatc gggaagatcc ggctctcacc gccgctcgcg cccgaatcgc tcgcgtggct
     5701 cagcgcggtg ccgcgcctca tcgcgtccaa caactgggtg atccgcggcg cgcgcagcga
     5761 atcaggcaag cccctgctct gcagcgaccc gcacctcgag gtgaaccgtc tcccgtcgat
     5821 ctggcaggag ctcgtgatgc gcctgccgga cgacacgatc atcggaagct cgatcccggg
     5881 cgcgccgggt ctgcccatcg gacggacgcg gcggcttgca tggggcgcca cctactcgtt
     5941 catggaccag atcgattttc gcatcgagga atgccgcgac ggggcctacc gtcgcggatc
     6001 atcatggaag ccgttcacgg tccgacgcga ggagattcgg ctcaagaagg gacggcccgt
     6061 tatcgaaacc gtgtacgaaa acgagcttgg ccctctcgaa ggagatccga acgtcgcggg
     6121 acattacctt gtgatgtgct ggtcggccgc gcgcggctgc atcgccaagg acttcaacgg
     6181 catcctgggc atgaccaccg caaagagcgt gaaggagggt atggccctct accgtcgcct
     6241 cgaggcgaac agcttcaact atgtgctcgc tgacgattcc ggcaatatcg gctatcagat
     6301 gagcggacgg ctgttcaacc ggccgcgcgg cgcaagcggt ctcgtcccgc tccccgcgtg
     6361 ggacacaaag tacaacagcg cggggttcgt gcccgccgcg aagctcccct cgcagtacaa
     6421 tcccgcgagc gggacgatcg tcacggcgaa ccaggacctc aaccgtttcg gaaaggcgaa
     6481 acccatcaac ctcccgatgg gatcgtatcg cgcggaccga atcgagcagc tcctgaaaaa
     6541 gaaatcgaaa tactcggtcg aggacatgaa ggcgattcac tacgacctgt actcgctgca
     6601 ggccgaagcc ttcatgaagc acctgcgccc cctcctgccc gacaccgaaa acggacgggc
     6661 gcttcgcgac tgggactgcg cgtacacggc agattccacg ggcgcgatgc tcttcgaatc
     6721 ggtgtatcac gcgctcctgc gagtcgtttt cggcgatggc ggcatggggc gcgaggtggt
     6781 ggagcacctc atggccgaga cgagcctctt caacgactac tacgcgaact tcgacaggat
     6841 attgctgggg aagcggtcgg catggttcaa cgaccgtccc aaagacgagc ttttccggcg
     6901 ggcgattgac gaggggctgt cggtaccggc cgcgccctat ggaaaaacca ggaagatcac
     6961 gctggcgcat ctgctcttcg gaggcaagct gccgtcgttc ctcggcttcg actacggtcc
     7021 aatcgagctt ccgggctgcc gtgcgaccat accccagggg cagatcttcc gcagcgcggg
     7081 gcgcaccacc accttcagcc cgtcatatcg catgatcgcc gatatggcga cacgcgagct
     7141 gcatacgaac ctcgccgggg gcgcctcgga tcggcgcttc tcgaaatggt acgtgaacga
     7201 tatgaagaac tggctgggcg gggtatataa gctgctgcgg tgaaataaaa aacgcacgcc
     7261 cggcaggagt cgaacctgcg acctacggat tcgaagtccg gcgctctatc caactgagct
     7321 acgggcgcgt ttatgatccg tggggcgtaa tatgcacggc cgctcgcgat gtcaagaagt
     7381 attgtcgctc cgaagcctcg catcggaacc ggacggacgt gcaagacgcg acaccatcgt
     7441 catcaacacc cggccggtat gtcgtatgaa cgcggcaccg tatgatccgc acctcacggt
     7501 gcgccataac aggcgataaa acgacgctct cgtgaagacg cgccgccgga gactccggac
     7561 ctcggcggcg atcttccgct cctcggacga catgcgggac gccgtcgctt cggtgaacca
     7621 gtccgagcat ccgtgcgatc cctcacgatc cgcaagcgcc gttcccggat aggccacggc
     7681 gaagttgatg tcgtaaaacg acagatcgag cgagcggatg aacgaagcgg ttttcccaaa
     7741 gtccgccatc gtttcgcccg gatggttcgc gacgaaaaag ccctcgagaa aaatccccgc
     7801 cgcgcgcacc gcttcggccg ccgcacggcc ctcctcgatt gtgcagcgct tgttcatcgc
     7861 catcaagacc tccggcgaac ccgactcgaa cccgatcccg atcgactcgc acccggaccg
     7921 ccgcagtgcg cgcgcgagct cggggtccat gcaatcggag cgcgtatagc accaatacga
     7981 caggcgcgcg cgcatcgacg ccaggcgctc gcagagcgca agggtatggt cccggtccag
     8041 cgtaaaggtg tcgtcgaaaa aatacaaata ccgcacgcca tggcgattcg cgaggagttc
     8101 gatctcttcc atgacgtgtt cgacggagcg acggcgcacg cgagttccgt gcaccagacg
     8161 cgaggcgcag aaggtgcagg cgaaggggca accgcgcgcc gtgatcatgc tcgctgaggg
     8221 tcgaaagcgc gaatagtagc gcggcatgtc cagcagcccc catgcgggaa acggcagcgc
     8281 gtccaaatcg gcgatcggcg cgcgcgtctc gttcacccgc acctcgccgt cgtccttcca
     8341 ggcgatcccc gccacgcccg ccggcgaacc gccgccgtcg agtacgtcgc acagctcggc
     8401 cagcgcgtac tcgccctccc ccatgaccac acagtcgatt tcgccgtgcg cgaccgaggc
     8461 gtctggaagc gcggtaacgt gcggccctcc gaacacgatc ttcgcacgcg gagccgcctc
     8521 acgaacgatg cgggccaacc cgaccgcgta ggcgaacatc ggcgacgtgc acccgaaccc
     8581 gacacaatcg ggttgatacg accgtacggc ccggtcgaac cggcgttcgc tgaacccatg
     8641 tacggcgtaa tcgagcacca tcacctcgtg gcgcgccatc agcacggcgg cgatgtgtgc
     8701 gatgccgagc gacgggagcg agtagacgcg tcgccgttcg gtcgccggcg gaatgacgag
     8761 cagcaccttc atcgacttca ccggtatcgc agcagcgcgc tgcgcgcaag cgaataatca
     8821 tttgacaatc ggcgccggga ggaccataat gttgcatgag cgcacagccc aaggcactcc
     8881 gcatcggcgg tattgtgatc gcgatccttg ccatcatcgc cgtcgtcgcc ctgtgcctgc
     8941 gtccgctggt gcggcggcac cgcctatcgc cggtcgtcac gagtgcgatc gagaagggcg
     9001 tcggctatct tgaacgttcc cagcttccgt cgggcgagtt ccggacctgt gcatcggccg
     9061 accccctgat gcgcgacgag tgcgccttcg acggctcccc gttcgccaca tcgctcatcg
     9121 tctactccct ctcgcgagcg tcccatccgg cgatcggcgg catcaccgag cgcgcgctgc
     9181 gcttcctccg cgacgacatg tccggcccgg gcatctggcg ctactggtcc cgcagcaatc
     9241 cgaagcatcg cgacatcgat cccgatctcg acgatatcgc ctgcgtatcg ttcgtcctca
     9301 ggaaattcgg cgcccccttc ccgccgaacg aacggctgct catcgacaac cgggcgggcg
     9361 aggggcgctt ccgcgtctgg ctcaggtccg gccgcttcca ggtgaacgaa ttcgactgcg
     9421 tggtcgatac gaacgtcctg ctctatctcg gcgaacggcc cgagacgcag gcggcctgcg
     9481 cgagcgtcaa cgccatcatc aacgccggta cgcgctgcgg gcggtactac ccgagcccgc
     9541 tgtcgctcta ctacatgcac tcgcgcgcgc ttcgcgaggg gattgcgtgc ctggggaaat
     9601 cacgggaggc ggcggtcgat ggcgtgatcc gtacggtacg cggacgcgaa tcatccggaa
     9661 acgcgctgca tgcggcgctc gccgcgtgcg ccctgctcaa ttatcagcgc gaaaaccgga
     9721 cgctcgaccg cttgatcgac gcgctcatcg agctgcagcg gcccgacggt tcatggcgca
     9781 gaagcacgtt ctatctcgga cccgcgccgt attacggatc ggacgagctg accaccgcgc
     9841 tcgcgctcga ggccctcgcg ggatatctga aaacaataca gcgctgaggg gccaatggaa
     9901 aaattgtatt gacccgcgcc cctcgggcat gcgaacatcc accgcgtcgt cacgtaaaat
     9961 cctcttcttc cggaaaccga cctatgcacg aacacgaaac gaaacaaaac cgcgggagcg
    10021 ccatcgtgag ccgatttcgc gcccttctcg acgatctcta catcggggaa aagacctaca
    10081 gccgggtctc cggctggctg atcaccatgg tgcgggcgag catcgcgacc acccggaaat
    10141 tcatcgagga cgactgcccc accatggcct catcgatcgc ctatacggcg atcgtctcgc
    10201 tcatccccac gctcacggtc gcgctcacct tctattcgct gctcgcgggc atgggcgcca
    10261 agaaggagga gctctttcgc cagatcatgg tcttcatgac cgaacacaac atcaagctga
    10321 acatcgaccc catgatcgag gcgatcacgg cgctcatcga caatgccggc aagatcggcg
    10381 gcatcggcgc ggtgattctc atcttctccg caacggccgt gcttcgcacc ctggagaaat
    10441 ccctgaacac catctggggg gtccagcaga accgtcccat catgcttcgg gtgatctact
    10501 actgggccgc gctcaccctg gggcctatca tgctcatcac cgggaccacc gtggccacgc
    10561 aggtatccaa cgccctctcg tccccgaact tccatgcggc ggccatcacc gagaccggcg
    10621 cgatatggac ggcggggaac aaggcgcgca tcctgagctc gaagggcgat ctcgcgttca
    10681 cgcgcatcga ccggcgctcc gtcgatttcg agaattcgcg cgtcatggaa tacgacaccg
    10741 cctcgcgcaa cttttaccag caggaatacc ggatcgagga attcgatttc gaccgcggct
    10801 cgttccgtga cgtgcagttc atgggcagca ggggatggat cgtgggcgcc aagggcatgc
    10861 tgttgacgac cgaggacggc gggcgccact gggcgctcgc gcgctgggga tcgtacaact
    10921 tcaacgccat tcacatgctc acgcccatgc gcggattcat cgccgccgac aacggcttcc
    10981 tgctctcgac aaccgacggg gggaactcct gggcggtccg cgactgggtg gacatcaccg
    11041 cgaacctttt cgccatcgac tttcgcggcc agcgcggcat cgtcgtgggc gagcgcggcg
    11101 ccttcattgc caccaccgac ggcggtgcga cctgggacgc gggcaagctc aatgagtcgc
    11161 gcgtgatgcg ccgccacgtc aacctgaacc acgtccggtt cgccaacgga tcggtctgga
    11221 tcgtggggga cgaggggctg atcctgcgca gcaccgacgg cgggaccaca tggaagagcc
    11281 ggaagcacct cgacatcaat tactttaccg cgtatttcat tgacgcgcgg cgcggctatg
    11341 tcgccgggga aaagggcgcg ctgctgtcga ccgacagcgg cggggagcgg tggacgcgcc
    11401 actcgcttgc gactccccgc gtaaaccgca tccagctcgc gaacgggacg ctgtgggcct
    11461 tcggccacaa cgggttcatc cagcgttcga ccgatggcgg tcagcgctgg tccggattgc
    11521 gcggcagcgg ttttttcatc ggcatcctaa atttcctcgc accgttcgtg atcatctggc
    11581 tgatgttctt tctcgtgtat ctggtgcttc cgcacgcgaa ggtccccgcg cgggccgcgc
    11641 tcatcggatc atcgttcacc ggcgcggtgt gggtcgcctt cacgctgctg ttcatcgtgt
    11701 acgtgaagta cttctcgtac ggaacgcacg ccatctacgg cgcgctggcg tccatcccgc
    11761 tgttcctgct gatgatttac tcctcggccg tcatttttct ttacggcgcg caggtctgct
    11821 ataccgcgat gcatccccac acctacctca agctcaaaaa gaagagcgcc accgaacgga
    11881 gcatgtcggt gtacaacggg atctccctct tgcatgcggt gtaccgccga ttcgaaagcg
    11941 gcggcggcgc catgtctctc cgcgagctcc agaaggcgac cggccagggc cctgaggagc
    12001 tcgagcgcta tctccgtatc ttttccaagg cccgtctgct gcgctacgac gccgatacgg
    12061 agatcgcccc gctcaacgca tcgtccaacg ttaccatcgc gtcggtcatc gggctattgc
    12121 acgactccgg actcacgccg cccgcgagcg cggccggacc ggcgcatctg cgcaagtacc
    12181 tgagcgaggt tcttgcggac attgcgaaat cgcgcgcgaa gatcgtgggg aacacgacgc
    12241 tcaaggatct gatcgcgaaa gcgccgtagc ggtcagtaaa tgaagtagcc gaacatgaag
    12301 gggatgacga cgatctgccc cttcacgtcc tcgggcacgg ggccgaagct cttggaccag
    12361 cggatcgatt ccacgcagga ctggtcgagc tggcggttcc cgagcgattc cacgatgacc
    12421 acgtcgagca cgttcccggc ccggtccatg gtgaaataga gcttcacgac ctggtcggga
    12481 atggcgttga tcttcacgta gccgggggca tagcccccga tcaccgactt ggccatatcg
    12541 ggcgggtgcc agttcgcggc gatcttgtcc ttcatctgct tgaagtagcg gaagttgcgg
    12601 aaccgtttgg tgttgaacga gaagcgcccg tcgttggaat agaagatggc gttgttcttg
    12661 ctgaaggtgt tgcggtcggg gaccttcgtg aagtcctcgc cgccctcgtc gccgaagagc
    12721 gtatagcccc cctcgctgag tttcatgaat tcgaccacca gttcgctctc gtcgttgagg
    12781 agcatgcgct tggggatctg cgagcttttg gtctggaccg tcgagctgtt cccgctcttc
    12841 ttcccgcctc gcttctgcgc gaaatcgcga gaattgttga gccagtggtc gcccttctcc
    12901 ttcgtgagat ggcccttggc cgacgagtcc tcatccgaca acagcgtctt gggcgagatg
    12961 accggctgcc ggtcggcgtt aatatttacg atgatatcgc gcccgccgaa catcttcctg
    13021 gtccgatcgc ggttcatctg gtcctgcagc atcgcgtaat aattggggat cacgatcccc
    13081 agcatcatga ccgtatgcag gaaaaacgac atgagcagga ccagggagag gttgatgtcg
    13141 cttcgccgcg tcatcgatgc cccatgccgc gccgttgcgg cgccgtgata tcgtggacgg
    13201 cctcgagcat gcccgtgaag gccggcgtac acgcaatgcc ccgtcgcgag gtcatcgcgg
    13261 cggccgcctt caggaatccc gggagcgaac cgcggtcgtg gagctcgttt tcgaaaaacc
    13321 acgcgaagga cggaacatgc gacggcggca tcgcgccggc gtggaccagc atgcacccga
    13381 ccccgatgct ggtcccggta ttgatgagcg tcccgatgct ggccttggag aaatcgccga
    13441 tgaaacagcc caccttgagc ttctccgtgt tcacccgccg gtcgggcacg tagcacttca
    13501 cggatgaata attgttcttg aggtcgctgt tggtggtgag cgcgccgagg ttgacccatt
    13561 cgccgatata cgaatggccg atgaagccgt cgtgatactt gttcgaattc gcctggaaca
    13621 ccgattcctc cacctcgccc ccgatacggc atccgggtcc gatgctgcat ccctcgcgca
    13681 ccttcgcgcc caggagcacg gccttcccgc cgatgaagca cggcccctcg atgcgcgtga
    13741 acgaattgat gacgcacccg tcgcccacga taatgggccc ggccgtgcaa tcgaaacaca
    13801 cgaacgggtc gatgcgtaca tcgtccccga tcagaaggag actcccgtcg ccgataattg
    13861 ttacctgcgg cggaagggga ttcggcgtcc gtttcgtata aaagcgcgcg tcccgggcga
    13921 tctgggcagg attcatctcg atgatgtccc agatgtagtc gaccgccgtc tccccatccg
    13981 cctcacggag ggcgactccg gaggagacga gcattttttc gatggaatcc gccgagtgtt
    14041 cggagaggcc gctcacgaca gcgtcggccg cggttgggtt caatcgcacg gcaagcgggg
    14101 cgccctgacg ggcgataccg tatcccgcct ggagatcgag cgccgccccg caatcgacat
    14161 accgcgcatt gatgaaaaga acgtccacgc cggaccgcag acgggagcaa tcgttgatga
    14221 cgatatcggg gtaccgctcg cggtagtatg cggcgaggtg ggggcgcgtt aaaaagacga
    14281 cggtcgatgc ggaacgggcg gatccgccct ccgaatcgag gaatctctga atgcgctcac
    14341 gcccggtgaa gcagccgcag cgaagctccc ataccggacg cgtgagcgac agcggataga
    14401 actgatcgac cagcccgtcc tcgaagacga cgacgttcat acaacgcgcc tgcgctactt
    14461 ggcggcctct tcctcgagct ccttcttgcg gtcctcgatc accttctcgg cgatcttgcc
    14521 ggagatggga tcatagtgcg agaacttcat ctcgaaggtg gccttgccgc tggtcttcgc
    14581 gcgaaggtcg atcgagtagc gcagcatctc ggcaagcggc accgtcgcct tgaccacgga
    14641 cacgccgccg cccttggatt catcggtgct gcccatgccc agcacgcggc cgcgccggct
    14701 ggtgatgtcg ttgaggatgt cgcccatgta gtctttatcg acgtagacgt cgacctccat
    14761 gatgggctcc aggagctgcg gcgccgctcc ctccatggcc ttcttgaagg cgccgcggga
    14821 ggcgatcttg aacgacattt cggacgagtc gaccgggtga tacgaaccgt cgaagacctc
    14881 gacggcgacg tcgaccaccg ggaagcgcgc gagcacgccc
//
LOCUS       DAORHF010000073        14846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3101_length_14846_cov_6.005409, whole genome shotgun sequence.
ACCESSION   DAORHF010000073 DAORHF010000000
VERSION     DAORHF010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14846
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_14846_cov_6.005409"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PKJ16_07990"
     CDS             complement(<1..654)
                     /locus_tag="PKJ16_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV46965.1"
                     /translation="MEHRTEYQKTWEILKPHRQMVFISGPRQAGKTTFARMLASEAPN
                     SLYFNWDLPDDKKRLIENPLFYEDVNRRDPSKPIVILDEIHKYHDWKNFLKGAYDRDG
                     AGYKFLVTGSGRLDLYRKGGDSLAGRYFLFHLWPFTLGELHGGTRSLDDFLRAPLECV
                     GGSTRSIRNAWESLAELSGFPDPFLSGKKQTYNIWSRAYHRQLVREDVRDLTGIKNIE
                     "
     gene            877..1077
                     /locus_tag="PKJ16_07995"
     CDS             877..1077
                     /locus_tag="PKJ16_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HNV46966.1"
                     /translation="MRLPGKAWLEFVDHGGRLLVTGYFIPKGVGGRVYWYALYPFHKV
                     LFRQMAKNIVARAKRDHNEQNH"
     gene            1281..1511
                     /locus_tag="PKJ16_08000"
     CDS             1281..1511
                     /locus_tag="PKJ16_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46967.1"
                     /translation="MKISRLIVALVLLIFMVSPLLAERVMNTTDKIIYIQADIRYIGF
                     TPGKVVLVTGKEFKSKGVQKAIKDGKLKVVSK"
     gene            complement(1918..2238)
                     /locus_tag="PKJ16_08005"
     CDS             complement(1918..2238)
                     /locus_tag="PKJ16_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46968.1"
                     /translation="MMVIEESVKKKGPRDSGKGKKETILYAYAVMAVLADGTVIEGTA
                     SLASPSLTFTHHKNGVAFEKTVRFDDIASIRAIEWKTVGRGEDPGEETRLYHFYPSRW
                     KVVF"
     gene            complement(2355..2555)
                     /locus_tag="PKJ16_08010"
     CDS             complement(2355..2555)
                     /locus_tag="PKJ16_08010"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNV46969.1"
                     /translation="MKKSAKDWLKQTENDLSWAHDTLRAGKYAQACFICQQVAEKALK
                     AIAYDRGCDMMKSHLVMEIARS"
     gene            complement(2727..3788)
                     /locus_tag="PKJ16_08015"
     CDS             complement(2727..3788)
                     /locus_tag="PKJ16_08015"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010607844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV46970.1"
                     /translation="MVLSRGENMETRITKLFGIKYPIILPGMSWISTPELVAAVCNAG
                     GIGWLATGPLSAEQTREAIKKIRSLTKKPFGCGCTLLMPGARENAEVMLEEKVPVINV
                     SLGKCDWIAERAHKYGGKVIATVTTVKHAMAAEAQGADAVQVTGNEAAAHGSQVTTMV
                     LVPAIARAVKIPVVGIGGAADGAGMMAILALGAEGVGMGTRLALTKESPVHQTTKDAH
                     VKAGIDDTIYSNRFDGLYCRVLDTPAAKKSIRQGMSLPRAGLASWKIAKAMNLPWIKL
                     AIGTMLQGPKLMMQLAHFATAFEKIQDATEFGDLKKGVALIGQVQGLIHDIPTVKQVI
                     DTTIAEAKEIEKRLAKSVK"
     gene            3930..4652
                     /locus_tag="PKJ16_08020"
     CDS             3930..4652
                     /locus_tag="PKJ16_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV46971.1"
                     /translation="MAVVEWKKDESVALVTMTNGENRHNPDFVKDMLGCLEQIKADAD
                     IKAVVVTSNDPKNFCLGVDINWLGGRMGEKDMESIKKWMYGMNDVFKAFLLFPKPVIA
                     AINGHAFGNGAIMACSCDFRFMRADRGFFCFPEVTIGIPFLPGMIAFVRKALPEYKFN
                     EMYLTGKRVGAAELAQHGIIEKASAGQDELMADAVGFAKTLSLKPGIFGEMKKRKHKA
                     IIDIMDREDPPYVDSLFLMVQD"
     gene            4713..5060
                     /locus_tag="PKJ16_08025"
     CDS             4713..5060
                     /locus_tag="PKJ16_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46972.1"
                     /translation="MAFMLMMPLLACTAEKKTAPAAAIKPPTSYEGIIVKRKFPLERA
                     SSLPGKNEHYILKTKDDLHIPLNPVISLMFPNLQSLLYRLVRVRGAYKSYPAGEFTVH
                     EITPLHDDSKSVP"
     gene            5017..5637
                     /locus_tag="PKJ16_08030"
     CDS             5017..5637
                     /locus_tag="PKJ16_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46973.1"
                     /translation="MRSRPSTTIRNPYRDEFLAAMERLGRHDGARTEELYRRRSELIG
                     RYAFAVPDDRAIGLLVRLAPIVEIGAGNGYWAWCLAQAGADVIALDKFSPDGALYDSA
                     APCGVENTWFDDEWRPVLRADETAAGAHPDRTLFLCWPDPRSYMAHTALAAYRAAGGR
                     RLVYIGDPASSGDPRFHAALETLACVERFRIESWPCCDEWCAHYEI"
     gene            5657..6022
                     /locus_tag="PKJ16_08035"
     CDS             5657..6022
                     /locus_tag="PKJ16_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46974.1"
                     /translation="MNATRISIALLAVVLAFAACGKADLNQKLIGAWETVGVKCNEQG
                     ACDPHGDPLSIEFRKDGGFVLEGNRFDYALSGRTATVRLGENVMTMEIIRISADEMLA
                     RTKSSLGLADIEKYRRKRP"
     gene            6019..6960
                     /locus_tag="PKJ16_08040"
     CDS             6019..6960
                     /locus_tag="PKJ16_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV46975.1"
                     /translation="MTAPGPRAIIDWLLESGRAIDTTPLPTLEDWLPRFARVRAEWTH
                     SADRAIAGGFLADRLAYAFAAGYESALGRLVPSLDDGATAALCVTEEGGGHPKAIRTT
                     LVRDGDASWRMTGRKKFVSMAADAKRLFVAATRGARDDGTSDLRLVAIDLPATGVAIE
                     LMKDLPFIPEIRHGALAIHNATVADDQILPGDGYTDYIKPFRTIEDIHVTAAAVAFAC
                     RCACLFGWPSPLRERMLTALVTLRTLSDDDPSSPAVHILYAGAAGMVAEIMREAQTLF
                     DAADEATHAAWARDSALLDVAGSARAARRAKAWEHYN"
     gene            7017..10982
                     /locus_tag="PKJ16_08045"
     CDS             7017..10982
                     /locus_tag="PKJ16_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNV46976.1"
                     /translation="MIRAITAQVVSFLAPRTWVLDDLWIRVPTIRRRVLAAALRPCLD
                     PDARTLRNKIDAIDGIIERLRRDQSLSRFFPPSGAHGPEPGPTSVVLNALPTTGMSRR
                     FELRNPHIIVVRRVRRFIDAGLRLYRRVFSRHVVPDESLREHLQSGLMEHGVAGVERW
                     FDFQELAIIDRRLLRLSERKPGRQRTPVFEKTRIAEFKGLLAPGIMARPVPASYIAHY
                     FPGFTPEEFIRGGAAVHEGRRPLFSITEVIALERVLVSRLRARNRASRLQRAVPLLKM
                     GAIVALCLWVALPLAFDTGILLYGFAKNRTAFSAVDSSRKIASPSSPYVVRANRVFRM
                     LEGAVADNFNRSGPAPVWPNRFAIDGGARLHVELNYGVYRALRVQDQTGLTVIAIDDR
                     KLAACRTADRDRYGRLAELIAGRPLAAGLAQWIARITGGRIVLHDPANVRERELRAKL
                     PDRIAAHHAPLEFIRSANDLCACVTVVYHKPNTQKWSPLDGIPDIMKKAVVIREDRRF
                     YNRLFPIPHRGNDNLVIVPQIAKKGLRDFLEWAHRVCARNRFDRLAKIAEGRMNRLTA
                     VLNVETRGGSSIANQVVELLFTKFIPEHSRAKTPIAGMIEQKKHELPASLALAWHWSR
                     DEILEAYVNEIYGGHLYSDIRGFQSQAEIYFTRPLAELTLREQMMLVGAIKKPSRIKE
                     LAQWQKAAELAALVEKTPGGADSRAVRDWTAANALYKVDPGNYREYLAVKLRAREWID
                     RRTNRILDLLLADGAISRREHRAAMAQRVINFNFAPPIVSLDTRLVNNIKRELDAELG
                     PGRSDSGAVVVTTIDAAMQASLQSIINTSAGHVYVDPDNRAEGQPAVVRIDGGARIIR
                     ANEMRGDAVTVVNRILADVGGSSAVEEEWDWITQANRSLGSSLKPLLVLYFLLEGYHL
                     SDELRNTSVTYANYTLEQQLKFLKFARRYPDRRDDIADIEKHWLWTPKNYRDYSDEWI
                     TVRRALANSVNSIHVQIQEIVTPDAFARLLNETMNITDERMRHKPFRSIILGGSSGDQ
                     RYDRFLLAFSLFANDGVIRKHTYLRSLLLPNGARLVPDYRLVRCPILERFGRERVRAA
                     ANLVNLAMRDAVTDGSMTAMNGIGAGKTGTSNELKDALATAHFVAGDDTYIVGVRLGN
                     KQNHSIGMAAAQIATPVAERIVRTVFDRASVVRGNAYDEYLLKKVRATPHIVESGRNF
                     FLAGEPPKSRRFSIAQMQRQTKDELAREALAHFKKRDYEEAAEAFERLLRIASWSDLA
                     DPAYRSMVECYRKLKNQERAGQIAELIATAKTDGAILERTGSLAEIVSRKKKGRGDRE
                     D"
     gene            complement(10986..11426)
                     /locus_tag="PKJ16_08050"
     CDS             complement(10986..11426)
                     /locus_tag="PKJ16_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46977.1"
                     /translation="MNTSKDTIGHNDQEILIRALVMYIVKKLKTSEGIVIGTFRMCIA
                     EGAKNLGKPFRDVRNLPDAERDAFYMGMFDMVIDRMKLNLAMRRAFKYDCLQIYERWK
                     ERTREMGTPPAESAAAPEIDQKKQDELLNDLDSLNLDDIEGFDL"
     gene            11839..12678
                     /locus_tag="PKJ16_08055"
     CDS             11839..12678
                     /locus_tag="PKJ16_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46978.1"
                     /translation="MMALCLVVAGTAVAQDSKAAKPEEKKTASDKESDKWESSEIQKE
                     RAKYKEDQRPEENYLAKFKSQEIVEFLIKENLEKIYMLKVVVSNFPQDSWNKEYTDIY
                     NGYKKAMSLFYKRDVIYSRVELEKNKKAINDLYKKMADKYRDDTEKMMDVCAETILII
                     SLNAKTKSDPNKMKEFYKNLMRIRIAYGQMDDAMNAYRDLNFQTSLFHFRVAKSYAIR
                     VMEELLDKEALEKNKDLYSQEEYSGFTKLTGEKDKFNIPVHKADNLNRILSEKQTATR
                     PTN"
     gene            12883..14235
                     /locus_tag="PKJ16_08060"
     CDS             12883..14235
                     /locus_tag="PKJ16_08060"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV46979.1"
                     /translation="MALRIAPTLACILVVVFTPAHFARHEFPVFRVVLDPGHGGRSMR
                     PISLYGDRYDSISGAYLDIYKEGAALYGIHEHVIMYAIARHAERMLAHCAPGGNFAAF
                     RAILARYTDVEPQRITIETSLSRPDSRNESAVRGRSDPNEQFRLFDFPGTNGTLREGR
                     LSVINARRPHLVVSLHCATDGPPKYRGLNPVIVAPYGMLYEGLRYLKGERQTPNFFYR
                     SAYRDWFSESSARPDIDWFIKDVSVYFTGYPPAGYPSKGSRFKGYRYNMVSWAYRDPA
                     GWEFRAWHHPDRTPYAIKYRGFNASNAFWERERSVYERHRRDGGPEGYGGDNHYASAE
                     IIRYVLYSLYVDAGRRSHPDERLTRPYISIWSVPLHVNAINAFVELGYLRIPHHRHML
                     SSRQLEIAEGIAVGVYSLLAGVTPRSSNLAQPPRGKRIDFGKYSISPSKNYFDCAAHD
                     "
     gene            14228..>14846
                     /locus_tag="PKJ16_08065"
     CDS             14228..>14846
                     /locus_tag="PKJ16_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV46980.1"
                     /translation="MINRTTIILLCAALSLAADRSAHLFVDAVRTAKPSVVNITMYQT
                     ARKNGEVQYAKTGYGSGTIITQSGYLVTNHHVVRKGTHYQIRLADGTECVPARLPNGQ
                     RYIADEKTDIAVMKLDAAGLGPLRPIELDDSDRLVEGEWVIAIGNPYGLHQSVSSGIV
                     SSKGRGDIGFADIEDFIQTDVPINPGNSGGPLVNLRGRMVGMNTAI"
BASE COUNT         2937 a         4537 c         4420 g         2952 t
ORIGIN      
        1 ctcgatattc ttgatgcccg tgaggtcccg cacatcctcg cggacaagct ggcggtggta
       61 ggcgcgggac cagatattat aggtctgctt tttccccgat agaaatggat cggggaaacc
      121 gctcagctcg gcaagcgact cccaggcatt gcgaatgctc cgggtggatc ctcccacgca
      181 ctcgaggggc gccctgagaa agtcgtccaa gctccgcgtt ccgccatgca gctctcccag
      241 cgtgaagggc cacaggtgga agaggaaata tcttccggcc agcgagtccc cgcctttccg
      301 gtagaggtcc agtctgccgc ttcccgtgac caggaattta tatccggcgc cgtccctgtc
      361 gtaggcgccc ttgaggaaat ttttccagtc gtggtattta tgaatttcat cgaggattac
      421 gatcggcttg gacgggtccc tcctgttcac gtcctcgtaa aacagggggt tttctatgag
      481 acgtttcttg tcgtcgggaa ggtcccagtt gaagtatagg gaattcggcg cctccgaggc
      541 gagcattcgg gcgaaggtcg tttttcccgc ctgacggggg ccgcttatga acaccatctg
      601 ccggtgaggc ttgaggattt cccaagtctt ctgatattca gtgcgatgct ccatggtgac
      661 tattttgtcg tgtaacctga aaaagtcaag actttttcag gttacactcg taaaaagaca
      721 atcaaggaaa ccccgcccct ttccgcaatg ggtcaatagg tatactgccg ttatgagaac
      781 tggatcagcc ctgcagacag tcccattccg gccaaaaagt gcgtgacacc ggacttaaaa
      841 tttgtggaga aagaacgatt gcttctgttg tcgcagatgc gacttcccgg aaaggcctgg
      901 ctggaatttg tagaccatgg cggaagactt cttgtgaccg gatattttat accgaagggc
      961 gtagggggac gcgtgtattg gtatgcgctg tatcccttcc ataaagtgct tttcagacag
     1021 atggcgaaaa atatcgttgc ccgggcgaag cgggatcata atgaacaaaa tcattaaata
     1081 ggtgtcgcgg acttgttggc gtgtgagggt ctgatgttcc ttggatggtt cattgaggac
     1141 tttacaagac caatttttgg ataatggtgg ggaagttaaa ttttcacaca gacagaggtt
     1201 attctgaaaa aattgtttac gtgtcgagaa gattgtccta tctcagtacg tgtgttttta
     1261 tatccctggg aggtactgtt atgaagattt caagactgat tgtagcactc gttctcctga
     1321 ttttcatggt gtcgcccctt ctcgcagaaa gggtcatgaa tacgaccgat aaaataattt
     1381 atatccaggc tgatatcaga tatatcgggt tcacccctgg taaagtggtt ctggtaacag
     1441 gtaaggaatt caagtcaaaa ggggttcaga aagcgataaa agatggtaaa ctcaaggtcg
     1501 tcagtaaata gtcaattatt tctatgattg ctcgaggggc cgttgcagat aaacggccct
     1561 tgtatatttt gattatatgg aaagccactc ttcccgtcag gagtcaagag acatacaatc
     1621 ggttatcaga aacagataac gggacctatg tcccggagtg aaatcgcagg catgataaaa
     1681 ttctcagtag cgtaatgatt atttaaactg caccaacatg cacgccttgc ggtcggggtg
     1741 atgccgggga taattgaaat cgctcaatcg cgcgttatgc cacatgttgg acgcccttcc
     1801 cgtttttttc cagtaatcca cgaagtaccc gtacacgacc gcgctcctgt cgcgattttt
     1861 tatggtaagc gtattgaaca gcgttatctg gcccttgaag tcgtagagct cgtccgatta
     1921 aaaaacgacc ttccatcgtg acggataaaa gtggtagagc cgggtttcct ccccggggtc
     1981 ctcgccgcgt ccgacggtct tccattcgat ggccctgatg ctcgcgatat cgtcgaagcg
     2041 aacggtcttt tcaaaggcaa cgccgttctt gtggtgcgtg aaggtgagcg agggcgaggc
     2101 gagagatgcc gttccctcga tgacggtacc atcggccaac accgccatca cggcataggc
     2161 gtacagtatc gtttcctttt tcccttttcc tgaatcccgc ggccccttct tctttaccga
     2221 ctcttcgatg accatcaagg aaactcagcc cctttccgca atgcgttaag agatatactg
     2281 ccggttatga gaactggatc aaacccgcaa ccagcccctt ccaagccaaa aagtcctata
     2341 cacaggtctt aaatttaact tctagcaatt tccatgacca aatgcgattt catcatatca
     2401 caccctcgat catatgcgat cgccttcagc gctttttcgg ccacctgttg gcagataaaa
     2461 cacgcctggg catactttcc ggcccgaagc gtatcatgcg cccatgatag atcattttcc
     2521 gtctgtttaa gccagtcttt cgcacttttt ttcacgatgg tgctattcct ttgtaatcca
     2581 cgctctcatc aatgccattg aaaaatacac cttcatgtcg ggcctgtcaa tacaaaaagg
     2641 gccggcattc gccgaccctt gtggtttatg catgcacgca cccgggacga ggcggtacct
     2701 tgtcccaacg cgcgcgctta ccgcaattat ttcaccgatt tcgctaaccg tttctcgatc
     2761 tccttcgcct cggcgatggt cgtgtcgatc acctgcttga cggtggggat gtcgtggatg
     2821 aggccctgca cctggccgat gagcgcaacg cccttcttga gatcgccgaa ttcggtggcg
     2881 tcctggattt tctcgaaggc ggtcgcgaag tgcgcgagct gcatcatgag cttggggccc
     2941 tgcagcatgg tgccgatcgc gagcttgatc cacggcaggt tcatcgcctt ggcgatcttc
     3001 caggaggcga ggcccgcgcg cggcaggctc atcccctggc ggatggattt cttcgcggcc
     3061 ggcgtgtcca gcacgcggca gtacaggcca tcgaagcggt tcgagtagat ggtgtcgtcg
     3121 atgcccgcct tcacgtgcgc gtccttggtc gtctggtgga ccgggctttc cttggtgagc
     3181 gcaaggcggg tgcccatgcc cacgccctcg gcgcccagcg cgagtatggc catcatgccg
     3241 gccccgtcgg ccgcgccgcc gatgcccacg accgggatct tcaccgcgcg agcgatcgcg
     3301 ggaaccagca ccatggtggt cacctggctg ccgtgcgcgg ccgcttcgtt gccggtcacc
     3361 tggaccgcgt cggcgccctg cgcctcggcc gccatcgcgt gcttcaccgt ggtcacggtc
     3421 gcgatcacct tgccgccgta cttgtgcgcc cgttccgcga tccagtcgca tttccccagc
     3481 gacacgttga tgaccgggac cttctcctca agcatcacct ccgcgttctc gcgcgcgccg
     3541 ggcatgagga gcgtgcagcc gcagccgaag ggcttcttgg tgagcgaacg gattttttta
     3601 atggcctcgc gcgtctgttc ggcgctcaac gggccggtcg cgagccagcc gatgccgccg
     3661 gcattgcata cggccgccac gagctcgggc gtgctgatcc agctcatgcc ggggaggatg
     3721 atgggatact tgatgccaaa gagcttggtg attctggttt ccatgttctc acctcttgat
     3781 agtaccataa cagtgttatt tttggatata tcgcgccccg ttcggtattg cgcaagacat
     3841 ttttccaaat aattgtggac acaaagagcg cgccccgcct aagtcgaaaa tcatagtgcg
     3901 taacgatttg tcacgagctg gaggagatca tggctgtagt tgaatggaaa aaggacgaga
     3961 gcgtcgccct ggtgacgatg accaacggcg agaaccgcca caaccccgat ttcgtgaagg
     4021 acatgctggg ctgcctcgag cagatcaagg ccgacgccga catcaaggcc gtcgtcgtca
     4081 cctcgaacga tccaaagaac ttctgcctgg gcgtcgacat caactggctc ggcggccgca
     4141 tgggcgagaa ggacatggag tccatcaaga agtggatgta cggcatgaac gacgtcttca
     4201 aggcctttct gctctttccc aagcccgtca tcgccgccat caacgggcac gccttcggca
     4261 acggcgccat catggcctgc tcctgcgact tccgcttcat gcgcgccgac cgcggcttct
     4321 tctgctttcc ggaggtcacc atcggcatcc cgttccttcc cggcatgatc gccttcgtcc
     4381 gcaaggccct tcccgagtac aagttcaacg agatgtacct caccgggaag cgcgtggggg
     4441 cggccgagct cgcgcagcac ggcatcatcg agaaggcctc cgcgggccag gacgagctca
     4501 tggccgacgc ggtgggtttc gccaagaccc tgagcctcaa gcccggcatc ttcggcgaga
     4561 tgaagaagcg caagcacaag gccatcatcg acatcatgga ccgcgaggac ccgccctatg
     4621 tggactcgct cttcctgatg gtgcaggact agcgggaacg gcccgtgcga atctcccgcg
     4681 ccatcactat gccccggcgg cgcattgcgt tcgtggcatt catgttgatg atgccgcttc
     4741 tcgcctgcac cgccgaaaag aaaacggcgc cggccgccgc gatcaaaccg ccgacgtcgt
     4801 acgagggcat catcgtgaag cggaagtttc cgttggagcg cgcgagctcg ctgcccggga
     4861 aaaacgagca ctacatcctc aagaccaagg acgatctgca cattccgctc aacccggtca
     4921 tcagcctcat gtttccgaac ctgcagtcgc tcctgtaccg gctcgtgcgc gtgcgcggcg
     4981 cgtacaagtc gtatccggcc ggggagttta ccgtccatga gatcacgccc ctccacgacg
     5041 attcgaaatc cgtaccgtga cgagtttctc gcggccatgg aacgcctcgg gcgtcatgat
     5101 ggcgcccgta cggaggagct ctatcggcgg cggtccgagc tcatcgggcg ctacgccttc
     5161 gcggtgcccg acgatcgcgc catcggcctg ctcgtccgcc tcgcgcccat cgtcgagatt
     5221 ggcgcgggga acggctactg ggcgtggtgc ctggcgcagg cgggcgcgga cgtgatcgcg
     5281 ctggacaagt tctctcccga cggggcgttg tatgattcgg ccgcgccgtg cggcgtcgaa
     5341 aacacctggt tcgacgacga gtggcgcccc gtcctgcgcg ccgacgagac ggccgccggg
     5401 gcgcacccgg accggacgct cttcctgtgc tggccggacc cgcgttccta tatggcgcat
     5461 accgcgcttg ccgcctatcg cgccgcgggc ggccgccggc tcgtctacat cggcgatccg
     5521 gcatcgtcgg gcgatccgcg gtttcacgcc gcgctcgaga cgctggcctg cgtggagcgc
     5581 tttcgcatcg aaagctggcc gtgttgcgac gaatggtgcg cgcactacga gatataagat
     5641 agacagagga aaccacatga acgcgacgag aatcagcatc gccctccttg ccgttgtcct
     5701 ggcgtttgcc gcctgcggca aggccgacct gaatcagaaa ctcatcggcg cctgggagac
     5761 ggtgggcgtg aagtgcaacg agcagggcgc ctgcgatccg cacggcgatc cgctctcaat
     5821 cgagttccgt aaggacggcg gcttcgtcct tgaggggaac cggttcgatt acgcgctctc
     5881 gggccgcacg gccaccgttc gtctcggcga gaacgtcatg acgatggaga tcatccggat
     5941 atcggccgac gagatgctcg cccggacgaa aagcagcctg ggactcgccg acatcgagaa
     6001 gtaccggcgg aagcgcccat gaccgcgccc ggtccgcgcg ccataatcga ctggctgctg
     6061 gagagcgggc gcgcgattga cacgacgccg ctcccgacgc tcgaggactg gctcccgcgt
     6121 ttcgcccgcg tccgcgccga gtggacgcat tcggccgacc gcgccatcgc cggcggcttt
     6181 ctcgccgacc ggttggccta tgccttcgcc gccggctatg aatcggcgct cgggcggctg
     6241 gtgccgtcgt tggacgatgg ggccaccgcc gcgctgtgcg taaccgagga gggcgggggg
     6301 catccgaagg cgatccgtac gacgctcgtg cgtgacggcg atgcgtcgtg gcgcatgacc
     6361 gggaggaaaa agttcgtgtc gatggccgcc gatgcgaagc gtctgttcgt cgccgcaacg
     6421 cggggcgcgc gcgacgacgg gacgagcgac ctgcggctgg tcgcgatcga cctccccgcc
     6481 accggcgtcg cgatcgagct aatgaaggac ctgccgttca tccccgagat acgccacggc
     6541 gcgctggcga tacacaatgc gaccgtcgcc gatgatcaaa tcctccccgg agacggctac
     6601 acggactaca tcaagccctt ccggacgatc gaggacatcc atgtgaccgc cgccgcagtc
     6661 gccttcgcct gccgctgtgc gtgtctgttc ggatggccct cccccctgcg cgagcggatg
     6721 ctcaccgcgc tcgtgacgct ccgcaccctg tcggacgacg atccctcgtc gccggcggtg
     6781 cacatcctct acgccggcgc cgccgggatg gtcgctgaga tcatgcgcga ggcgcaaacg
     6841 ctcttcgacg ccgccgacga ggcgacgcac gcggcatggg cgcgcgacag cgcgctcttg
     6901 gatgtcgccg ggtcggcgcg ggccgcgcgc cgcgcaaagg cctgggaaca ttacaattga
     6961 caaatcgccc cgtgcgccaa tagtccgtcc agaaccgatc cgcgcaccgc gattccatga
     7021 tccgagccat cacagcacag gtcgtatcgt tcctcgcgcc ccgcacctgg gtgctcgacg
     7081 acctgtggat ccgcgtcccg acgatacggc ggcgggtcct cgcggcggcg ctgcgcccgt
     7141 gcctcgatcc cgacgcgaga accctgcgga acaagatcga cgcgatcgac gggatcatcg
     7201 aacggctcag gcgcgatcaa tccctgtcac gctttttccc gccctccggc gcacacggcc
     7261 cggagccggg tccgacgtcc gtcgtcctga acgcgctccc gaccacgggg atgtcccgcc
     7321 gcttcgagct gcgcaatccg cacatcatcg ttgtgcgtcg ggtgcgtcgg ttcatcgacg
     7381 ccggactgcg gctctaccgg cgcgtctttt cgcggcatgt cgttcccgac gaatcgctgc
     7441 gggaacacct gcaatccggt ctcatggaac acggtgtcgc gggggtcgag cgatggttcg
     7501 atttccagga gctcgccatc atcgaccgcc ggctcctccg tctctctgag cgcaagcccg
     7561 gtcggcaacg tacgcccgtg ttcgaaaaga cgcgcatcgc cgagttcaag ggcctccttg
     7621 cgcccgggat catggcgcga ccggtccctg cgagctatat cgcgcactac ttccccggtt
     7681 ttacgccgga ggagttcatc cgcggcggcg cggccgttca cgagggacgg cggccgctgt
     7741 tctcgatcac ggaggtgatc gcgctcgagc gtgttctggt gtcgcgcctg cgcgcgcgaa
     7801 atcgcgcaag ccgcctgcag cgcgcggtcc cgctgctcaa gatgggggcg atcgtcgccc
     7861 tgtgtctctg ggtcgccctt ccgctcgcat tcgacaccgg tattttactg tacggattcg
     7921 caaagaaccg gaccgccttc agcgcggtcg attcctcgcg gaagatcgcg tcgccgtcct
     7981 cgccgtatgt ggtgcgcgca aaccgcgtgt tccgcatgct cgagggcgcc gtcgccgaca
     8041 attttaaccg gtccggtccc gcgcccgtat ggccaaaccg gttcgcgatc gacggcggcg
     8101 cgcgcctgca cgttgagctc aactacggcg tctaccgcgc ccttcgcgtc caggaccaga
     8161 cggggctcac ggtgatcgcc atcgacgatc ggaagcttgc cgcgtgccgc accgccgacc
     8221 gggaccgata cggccgcctc gccgagctga tcgccggacg gccgcttgcg gccggtctcg
     8281 cgcagtggat cgcgcgcatc accggcgggc gtatcgtcct gcatgatccc gcgaacgtgc
     8341 gggagcgtga gcttcgcgcg aagctcccgg accggatcgc cgcccatcac gcgccgctcg
     8401 aattcatccg ttcggcgaac gatctgtgcg catgcgtgac ggtcgtgtat cacaagccca
     8461 atacgcagaa gtggtcgccg ctggacggga tcccggacat catgaaaaaa gcggtggtga
     8521 tccgcgagga ccgccgcttc tacaatcgac tgtttcccat accgcaccgg ggcaacgaca
     8581 atttggtcat cgttccccag atcgccaaga aggggctgcg cgattttctc gaatgggcgc
     8641 accgcgtgtg cgcgcgcaac cggttcgacc ggctggcgaa gatcgcggag gggcggatga
     8701 accggctcac ggccgtgctc aacgtggaaa cgcgcggcgg gagctccatc gccaaccagg
     8761 tggtcgagct gttgttcacc aagttcatcc cggagcactc gcgcgcgaag acgcccatcg
     8821 ccgggatgat cgagcagaag aaacacgagc tccccgcgtc gctcgcgctc gcctggcact
     8881 ggtcgcgcga cgagatcctc gaggcctatg tgaacgagat ctacggcggc cacctgtatt
     8941 ccgacatacg cggcttccaa tcgcaggccg agatctattt cacgcggccg ctcgcggagc
     9001 tcacgctgcg ggagcagatg atgctggtgg gggccatcaa gaagccgtcg cggatcaagg
     9061 agctggcgca gtggcagaag gccgccgagc tcgcggcgct tgtcgagaaa acgcccggcg
     9121 gtgcggactc gagggcggtg cgcgactgga cggcggccaa cgcgctgtac aaggtggatc
     9181 cggggaacta ccgcgaatac ctcgccgtga agctgcgcgc ccgcgaatgg atcgatcgcc
     9241 gcaccaaccg catcctcgac ctgctcctgg ccgacggggc tatctcgcgg cgcgagcacc
     9301 gcgcggcgat ggcgcagcgc gtgatcaact tcaattttgc gccgcccatc gtttcgctcg
     9361 acacccgcct ggtgaataat atcaagcgcg agctcgacgc ggagctcggg ccgggacgct
     9421 ccgattcggg cgcggtcgtc gtcaccacga tcgacgcggc gatgcaggcg tcgctccagt
     9481 cgatcatcaa tacaagcgcc gggcatgtct atgtcgatcc cgacaatcgc gcggagggac
     9541 agccggccgt cgtgcgcatc gacggcggcg cgcgcatcat acgggccaat gagatgcggg
     9601 gggatgcggt caccgtggtc aaccggatcc tcgcggacgt gggaggcagc tcggcggtcg
     9661 aggaggaatg ggactggatc acgcaggcga accggtcgct cggatcgagc ctcaagccgc
     9721 tcctggtgct ctacttcctg ctcgagggat accatctcag cgacgagctg cgcaacacct
     9781 cggtcacgta cgcgaactac accctcgagc aacagctcaa gttcctgaag ttcgcccgcc
     9841 ggtacccgga ccggcgcgac gacatcgccg acatcgaaaa gcactggctc tggacgccca
     9901 agaactatcg cgactattcg gacgaatgga tcaccgtgcg acgcgcgctc gccaactcgg
     9961 tcaacagcat ccacgtccag atacaggaga tcgtgacgcc ggacgccttc gcgcggctcc
    10021 tgaacgagac gatgaacatc accgacgagc gcatgcggca caagcccttc cgctccatca
    10081 tcctcggcgg ctccagcggg gatcagcgct acgaccgctt tctgctggcc ttttcgctct
    10141 tcgccaacga cggcgtcatc agaaagcaca cctacctgcg atcgctgctc ttgccgaacg
    10201 gcgcccgcct cgttcccgac taccgactcg tccgctgtcc catcctggag cgattcggcc
    10261 gcgagcgcgt ccgggccgcc gcgaacctgg tgaatctggc catgcgcgac gccgtgacgg
    10321 acggctccat gaccgccatg aacggaatcg gcgccgggaa aaccgggacc tccaacgaac
    10381 tcaaggatgc gctggccacc gcgcatttcg tcgccggcga cgacacctat atcgtgggcg
    10441 ttcgcctggg caacaagcag aaccattcga tcggcatggc cgcggcgcaa atcgccacgc
    10501 ccgtcgccga gcgcatcgtg cgcacggtgt tcgatcgggc gtcggtggtg cgcggcaacg
    10561 cctatgacga gtatcttttg aaaaaggtcc gcgcgacgcc gcacatcgtc gagtcggggc
    10621 gcaatttttt cctcgcgggc gaaccgccga aatcgcggcg cttttccatc gcgcagatgc
    10681 agcggcagac gaaggacgaa ctcgcgcggg aggcgcttgc gcatttcaag aagcgcgatt
    10741 acgaggaggc ggccgaggcg ttcgagcgcc tgctgcgcat cgcctcatgg tccgatctgg
    10801 ccgatccggc ctaccggtcc atggtcgagt gctatcggaa gttgaaaaat caggagcgcg
    10861 cgggccagat cgccgagctc atcgcgaccg ccaaaaccga cggcgcgatc ctcgagcgga
    10921 cgggctcgct ggcggagata gtctcgcgga aaaaaaaggg ccggggcgac cgggaggatt
    10981 gacgcctaaa ggtcgaatcc ctcgatgtcg tcgaggttga ggctgtcgag atcgttgagc
    11041 agctcgtcct gtttcttctg gtcaatctcg ggcgcggcgg cgctctcggc ggggggcgtt
    11101 cccatctccc gcgttcgctc cttccagcgc tcgtagatct gcaggcagtc gtatttgaag
    11161 gcgcgccgca tggcgaggtt cagcttcatg cggtcgatga ccatatcgaa cattcccatg
    11221 tagaaggcgt cgcgctcggc gtccggcagg ttccggacgt cccgaaacgg cttgccgaga
    11281 ttcttggcgc cctcggcgat gcacatccgg aacgtcccga tgacgatccc ctcggaggtc
    11341 ttgagcttct tcacgatata catcacgagc gcccggatga ggatctcctg atcgttgtga
    11401 ccgatggtgt ctttcgaggt gttcatcatg cattcactgc gttgttcgat tgcgcccgca
    11461 tacactcaat atccgcgtgg tgcgtaaaat acaatcactt ttagactttt tttgacaaat
    11521 gtcaattcgg cgcgatggga ttgaaaaatg aataattttc aatcagccta aaaaaacact
    11581 aaagtttgca ccgcgccata cgcacgatac gccggttcga cgttcacgaa tcattaattg
    11641 tatcgtttgg aacgatatcc cgatggcata ttatttttgt aaaaaataaa aaaaaaagtt
    11701 gcgtatatta aatttgttaa atacgttcaa cataactggg cttatatggt cgcaaacagg
    11761 gatgtttgcg aataatttca tatgtaccaa aaggagtgtt ttatgaagac cagcacgatg
    11821 agagtttttg ggattatcat gatggctctc tgtcttgttg ttgccggcac cgctgtggcg
    11881 caggacagca aagcagccaa gccagaggaa aagaaaacgg cgtccgacaa ggaatcggac
    11941 aaatgggaat cgtccgaaat ccagaaagag cgggcgaagt acaaggaaga ccaacgccca
    12001 gaagaaaact acctggcgaa attcaagtcc caggaaatcg tcgagttcct catcaaggaa
    12061 aacctcgaaa agatttacat gctcaaggtt gtcgtgagca acttcccgca ggattcctgg
    12121 aacaaggaat acactgacat ctacaacggc tacaagaagg cgatgagcct tttttacaaa
    12181 cgggacgtca tttattcgcg ggtggagctc gagaagaaca agaaggcgat caacgacctg
    12241 tacaagaaga tggccgacaa gtatcgtgac gacaccgaaa agatgatgga tgtgtgcgcc
    12301 gaaacgattc ttatcatctc tctgaacgca aagacgaaat cagatcccaa caagatgaag
    12361 gagttctaca agaacctcat gcggatccgg atcgcctacg ggcagatgga cgatgcgatg
    12421 aacgcctacc gggatttgaa ttttcaaacg tccctgttcc atttccgcgt ggcgaaatcg
    12481 tacgccatcc gcgtcatgga agagctcctc gacaaggaag cccttgagaa gaacaaggac
    12541 ctctacagcc aggaggagta cagcggtttc accaagctga ccggtgagaa ggacaagttc
    12601 aatattccgg ttcataaggc cgacaatctg aaccgcattc tgtccgaaaa gcaaacggcg
    12661 acgcgcccga ccaactaacg tcgtcgcgga cgcggtttaa ttcaacagac gcatgtgcgc
    12721 aaaaagggga gggtgctccc ctttttgcat ttggacggtc gcccgactcg gggagggctt
    12781 gacatgccgg cgggcgccgg atcaaatcgc gccatggtcc cgtttctcgc caacatgaag
    12841 gcagtatgca ttcgcgcccc acatgcagca acggtggcgc gtatggcgct acgaattgcg
    12901 cccacgctcg cgtgtattct cgttgtcgta ttcacacccg cccatttcgc ccggcatgaa
    12961 ttccccgtgt ttcgcgtcgt tctcgatccg ggtcatggcg gccggagcat gcgccccatc
    13021 tccctctacg gcgatcggta cgattccatc tccggcgcgt atctggacat ttacaaggag
    13081 ggcgcggcgc tctacggcat ccatgagcat gtcatcatgt acgcgattgc ccgccatgcg
    13141 gagcggatgc tcgcccactg cgcgccgggc ggcaacttcg cggcctttcg ggcgatcctt
    13201 gcgcgctaca ccgatgtcga gccgcagcgc ataacgatcg agacgtcgct gagccgcccg
    13261 gattcgcgca atgaaagcgc ggtccgcggc cgttcggatc ccaacgagca gtttcgcctg
    13321 ttcgattttc ccggcacaaa cggtacgctg cgcgagggac ggctctcggt catcaatgcg
    13381 cggcggccgc acctcgtggt gtcgttgcac tgcgcgaccg acggaccgcc gaaataccgt
    13441 gggctcaatc cggtcattgt cgcgccatac ggcatgctgt acgagggatt gcggtatctg
    13501 aagggcgagc gccagacgcc gaatttcttc tatcgtagcg cctatcgcga ttggttttcg
    13561 gagtccagcg cgcggccgga catcgattgg ttcatcaagg acgtgtcggt gtatttcacc
    13621 ggatatcccc cggcggggta tccgagcaag gggagccgtt tcaagggata ccggtacaac
    13681 atggtttcgt gggcctatcg cgatcccgcc ggctgggagt ttcgcgcctg gcaccatccc
    13741 gatcggacac cctatgcgat aaagtaccgc ggcttcaatg cgagcaatgc cttctgggag
    13801 cgcgagcgtt cggtgtacga gcgtcatcgg cgggacggcg ggccggaggg ctacggcggc
    13861 gacaatcatt atgcctcggc ggaaatcatc cgttacgtgc tctattcgct ctacgtggat
    13921 gccgggcgtc gcagccatcc cgatgaacgg ctcacccgcc cctacatttc gatctggtcg
    13981 gtcccgctcc atgtgaatgc gatcaacgcc tttgtcgagc tggggtatct gcggattccc
    14041 catcatcgcc acatgctctc cagccggcag ctggagatcg cggagggtat cgctgtgggc
    14101 gtctattccc tcctcgcggg cgtcacgccg cgatcatcaa acctcgcgca gccgccccgg
    14161 ggaaaacgaa ttgacttcgg caagtattcg atttcaccat cgaaaaacta tttcgattgt
    14221 gccgcgcatg attaaccgta cgaccatcat cctcctgtgt gccgcattgt cgctcgcggc
    14281 ggatcgttcc gcgcacctgt tcgtcgacgc cgtgcggacg gcgaagccga gcgttgtcaa
    14341 tatcacaatg taccagaccg cgagaaagaa cggcgaggtg cagtatgcca agacgggata
    14401 cgggtcgggc accatcatca cccagagcgg gtacctggtg acgaaccatc acgtggtccg
    14461 caaggggacc cactaccaga tccggctggc cgacgggacc gagtgcgtcc ccgcgcggct
    14521 gccgaacggc cagcgctata tcgccgacga gaagaccgac atcgcggtca tgaagctcga
    14581 cgccgccggg ctcggtccgc tgcggcccat cgagctggac gattcggacc ggctcgtcga
    14641 gggggagtgg gtgatcgcga tcggcaatcc gtacgggctc caccagtcgg tctccagcgg
    14701 aatcgtgtcc tcgaaggggc gcggcgatat cgggttcgcc gacatcgagg atttcatcca
    14761 gacggacgtt cccatcaatc ccggcaattc gggagggccg ctggtgaacc ttcgcgggcg
    14821 gatggtgggc atgaacacgg ccattc
//
LOCUS       DAORHF010000074        14713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3150_length_14713_cov_4.927889, whole genome shotgun sequence.
ACCESSION   DAORHF010000074 DAORHF010000000
VERSION     DAORHF010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14713
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_14713_cov_4.927889"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..3948
                     /locus_tag="PKJ16_08070"
     CDS             178..3948
                     /locus_tag="PKJ16_08070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46981.1"
                     /translation="MSESNISILLVDDEAIIALATQRVLEDAGYAVITAHSGREAVAV
                     ATADDAAIDLILMDVDLGDGMDGTATAREILARHEVPVLFLSSHTEKEIVRRTEEISS
                     YGYVVKNSGDVVLLASIKMALRLHAANRKLEHQSEELEAANEELNATIEELEQANEEL
                     QRSESDLMERERVLHESELLLDHTGEMARVGGWQLVPATLEVTWTRETYRIHEVPIGQ
                     RPSLEEAINFFHPDDRGRLSEAIARAIEHGEPYDMEMRFITAKGNRLWTRTRCIPEVE
                     HGKTVLLRGIFQDITERKQNELAMRESEEHYRAIVENTSDFIMRYDREGRHLFGNRAA
                     LEVTGVTLDEYVGKTHREMGFPEDLCALWEEGIHAVFETAEPRAVNFDVALPGGTMSL
                     ELKLFPEFGADGSVASVIGVSRDMSEHVRTADALHERIKELDCLYGVADIIESPGAAL
                     EDVLRGIAALIPPALSRPEAYGARIVAGERDYASPGFTARTPLCAIDITEHGAPYGTI
                     EVHGDAAHPLLPEETRLIEAVAERTSRMIERTIAEGRLRESDELIRALRDSRLTLMYT
                     HDLEGNFTDADDGLLELLGYRREEIRSINFASLIGDEQLEKALRILQDLISTGRQSAP
                     AEYLLHARDGREITIETIGFPIYRNGRLSAIIGIARDITEQKSMEEKLRSSEAKFRSL
                     FENITIGVAIHEIVVDDNNNPIDFTWLDCNPAYENMTRLHRSEIVGKRGLDIIPSLEH
                     TWISTYGRVALSGEPVMITDHSEYLDRYWEVYAFSPKLGFFAVAMKDITEHKRMEEEL
                     RASTDRLVRAEFASHSGNWEFHLATGSVFASEGARAVYGLWNENWTISEVQKVPLPEY
                     RALLDEAMGSLMDGTRPYDVEFRIRRPSDGEVRDIHSIAEYDRERNVVFGVIRDVTEQ
                     KRAERAAAANEERYRMLFEHDLTGDFVFDVTRERIVDCNDAFVALLGFTDRAAVQNGA
                     EGFHALDLPELRASFDALRTSRFISHHEVRMHRRDGAEVHLDQNLVGIFDQAGTLSLV
                     LGYFYDITARKHYEAKLRQSLEDKNMLMRELQHRVKNNLNLVSALLGLEMGNLVDQGA
                     RNIFSNMQNRIASMAKLYEQLHGADSTERVSMKEYVASVSLSLSSALTGGMRGIRIRA
                     TADDIVLDMRQAVPLGIIMNELATNAIKHAYPWDAPGEIRITLTREDGHAALTVADDG
                     RGLPPGFSIEDAKSMGMQLVGILARQIRGTVTVESGPGTTVRVLFPLPHPLR"
     gene            4170..7478
                     /locus_tag="PKJ16_08075"
     CDS             4170..7478
                     /locus_tag="PKJ16_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNV46982.1"
                     /translation="MHGENTSPVFYVGIGASAGGLEALESFFSAMPADSGMAFVVIQH
                     LSPDYKSLMVELLSKRTAMPVRRAEEGMLVEPNTVYLIPPKKELTIFHGKLILREQDH
                     NRGINLPIDVFLRSLADDQAEKAIGIILSGTGSDGVRGIRAIKETGGMIMVQSEESAK
                     FDGMPRSAISTGLADVVLAPDEMPGRLLSFMKCPYAAAERPQALLSDEDGITRIFALL
                     RDQMKVDFTFYKPSTVLRRIERRMTVNQIQDLRDYVRFLELRPGEVSTLHRELLIGVT
                     SFFRDREVFDELCEKQLPALLDSIDQREVRFWVAGCSSGEEAYSLAILAREYLDRSRR
                     TLNVKVFATDIDRDALVHAGNGLYAESIAADLPPGVLTRHFTHRDDRYQVNRTIREMV
                     VFAQHNLIKDPPFTNIDLLSCRNLLIYLQPVLQQKVMEFFNFSLNPQGILLLGTSETP
                     GDMSDFFEPLHHKHKIYRSKGKKRPPLERHFAPHPTKTAGGRYAARLGRGAQALLQRE
                     EERVMERLLQAMSDNADSMAAVVNERLELLHIAGSPDGYLRLPSGKLHNDIARLSVRE
                     IAIPLTTGLQKVFATGEAVRYTNIRVTRGGAERMAHMHIRLLPGRTGQEPLAGVFLQD
                     MPVARPEPAPGAGHEFDISREAEQRIGDLEQELQFTKENLQATIEELETSNEELQATN
                     EELLASNEELQSTNEELQSVNEELHTVNAEYQIKILELTEMTNDLDNLIAATGIATLF
                     LDENLDIRKFTPELKRIFRILEGDVGRPVTHLTHAIDGADPIEAVRRVTETGAPEERE
                     VRTADGSWFLMRALPYYVNESSVSGVVLTFTDIGTLKRAEQAVSESESRYRELFESMR
                     DGYALHEIITDDAGVPADYRFVAVNPAFERLTGLAASDIIGKRVLEAIPGTERHWIET
                     YGRVALTGEPLSFESYSGAIDKHFDVYAFRPAEGMFACVFRDITTRTRALDRLRKVTD
                     VLNATQELTKTGGWEWDVEKREMFWTEEVYRIHRIDPGAIPPGSTEHIDRSVECYDEA
                     DRPVIAEAFRRCVEEGVAYDRTFFFTDTQGRRKRIRTAAHPVVSDGRIVRVIGNIVEL
                     NDSNTYCNDRTETG"
     gene            7513..9996
                     /locus_tag="PKJ16_08080"
     CDS             7513..9996
                     /locus_tag="PKJ16_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46983.1"
                     /translation="MGNDNDRADLRRRAEESLRASPPEARPRTPGEADQMIQELQVHQ
                     IELEIQNDELRKTQENLVASRDRFSRLFNNAPAGYLVLDESGLIVEANETFCRMAAVR
                     RDRALGRGIADFIAGADRHGFLSRYRAFYNNPSGKTLEAQLASGDSVAWVRMEGSRIS
                     AIDAADPEARQLLLSMSDITQQKRAEAALAERERYLQKILQTSADGFWVLDMNGRFTD
                     VNDAYCRMSGYGREEFLALAIHDIDAIEGPEETAKRIGRIIEKGTDIFETRHRRKDGS
                     AFDVEVSVAYWESGGGQFVCFCRDITERKRNELQLRERLKEMRCHYAFSDLLDSHPPI
                     DADMLRRIAELIPPAMLHPEIAAARITVDGIAAESGDCPDTPWVIATDIMIHDRRAGA
                     VTVCYREDSPSPISDSAPFLPEERQLIDDIAERLGRHIEKVRAEEALRRRINENDALL
                     MASRAVLEQRDFTATARTIFDIGKNITGATAGYVALLSATGEENEVLFLDAGGRPCTV
                     DPYLPMPIRGLRAESYRHRKTVFDNDFHNSEWMSFMPAGHVRLDNVMFAPLIVEGTAV
                     GIIGLANKDGGFTDEDARIATALGDIAAIALMNSRNLEKLKASIGERDVLMRELQHRV
                     KNNMNIITSLLSLEMPNLADENARRIFKNMQGRIASMAKLYQQLYSTGSYSRVSMNDY
                     MDAITASINSSLGGGARGVRISAASDDITLEMNQAVPLGLVMTELATNALKYAYPPDA
                     PGEIRITLSRAGDAAVLTVSDDGPGLPPGFSIQNAKSMGMQLVDILARQIKGTVSVES
                     GPGTTVRVTFPTSSASTGSALTSPPAPLP"
     gene            complement(10104..10493)
                     /locus_tag="PKJ16_08085"
     CDS             complement(10104..10493)
                     /locus_tag="PKJ16_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HNV46984.1"
                     /translation="MGNSGIIKGQSGAGRKTERARELRKEQTAAEEVFWGRVRNRKFK
                     SLKFRRQQVIDGCIVDFYCEELKLCVEIDGSVHDNEQQVTYDAHRDKILSHRGIRIVR
                     LRNEAVHADVDRALDELYESISFERLS"
     gene            complement(10565..11269)
                     /locus_tag="PKJ16_08090"
     CDS             complement(10565..11269)
                     /locus_tag="PKJ16_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV46985.1"
                     /translation="MKTAETARTAKRILIVDDHPLICRGLAQVIDQEGDLRVCGDAGD
                     INDAMKKIESLAPDLVVVDISLKSSSGIDLIKAVRARNPRLPAIVYSMHDESVYAERA
                     LRAGANGYVMKDRPAEVLISAIRKVLDGNKFFSDEVISRILGRIAEEPPGNAGDPIAR
                     LTDRELEVFQLMGRELKTAQIAKKLNISVKTVEAHREHIRKKLDIENSHELARRAREW
                     VTSSPRPPSPIGEGGA"
     gene            complement(11337..13832)
                     /locus_tag="PKJ16_08095"
     CDS             complement(11337..13832)
                     /locus_tag="PKJ16_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV46986.1"
                     /translation="MSARILIVEDELVVARGIGEMLTRFGYAVVGTADRGEEAVDLAG
                     RLAPDLILMDIVLAGAMDGVEAAREIRERFAVPVVYMTANADMGTVARARDTAPYGYV
                     LKPVERLHLFSTIDTAVNKHALERRLVESERTARALLDASRDRAFLIGRDYVILGLNI
                     VAAQAVGMRADEAIGRSILDLVPPDLAASRKPHLDRALATGMAVTFEDERGGVLLEHH
                     IYPIRDERGATAQIAIFARDVTIERRAQEAIRKSEERYRRIVETAAEGVWSMDGDRVT
                     TFVNRQMAEMLGRPAVDIVGKRVTDFMFPEDLAEHERRMEGRARGEGGRYEQRFRRAD
                     GSTLWCLVSATALAGDDGSFHGSFAMFTDITQRKQAEERLQNNQRILFSLLNATTDSA
                     ALLDVDGVTLMENDAFRLDFGDDYSVLIERAVPEAPQGPIVRGAVSRVEAVVATGRPV
                     NFMLRRDNRHFDVTLYPIVDANRFVVNIALFAHDITTIVRAEQELRASEEKFRCLFED
                     SSDAQALLDRFAVVDCNGAAAQLLEYGGARDLVGKSLYDCSPEVQPDGIFSEAKGDLV
                     SRRLAEEGSFRFEWELVRRGGSSFIVEVYATRIRLGSRTVVHAAFRDITERKRLQNEI
                     IRIIDIEQERLGRDLHDGIGQELTGIAFLGASLAKRLKDSAHPLAGDAEEVVRHVYEV
                     ISRTRGLSRELYPPTLVENDMVYAVRDFVEGVRTVFSVECEFEMDPGLSITDKTASAQ
                     LYFIVREAVNNAIRHGNPKRIAIRLTSEGENGTLVIEDNGAGPPETLDAATGLGLRIM
                     KYRMESLSGTARFYRNESGGATVHCTFPLSAIE"
     gene            complement(13829..>14713)
                     /locus_tag="PKJ16_08100"
     CDS             complement(13829..>14713)
                     /locus_tag="PKJ16_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNV46987.1"
                     /translation="GQDEYRVFKARRLPSREEIAPDDWALAHTINEGVTIANELLEID
                     AFDGVTRTVLNFTAPVRDDSGAVLGALVVNQDITDLKRAQDAIVDSLNEKETLLKEIH
                     HRVKNNFQLIMSLLDLQANRQEAREVIAQFATAQGRIKSMALVHEKLYQSADLGRIDF
                     AEYLGDIVQSMAMYCPRGKRVPAMRVDAHPVYLNIERAVPCGLVVNELLVNACKHAFP
                     DGWAGEPELFVSLREEAEDRVCLTVADNGIGLPEGAEDGTGNALGFILVGLLVKQLRG
                     ELAIDRSNGTRFTVRFGR"
BASE COUNT         2818 a         4896 c         4528 g         2471 t
ORIGIN      
        1 gccctctcct atgggagagg gcggcccaga gggcgggtga ggtcacgata gccgcataac
       61 gacgatagaa atatatccag cccaaacgat ttatagagcc cgtccctata ggggagcacc
      121 tatagggttt tcatttttgt gttgcgaaaa taccaaagcc tttcataagg tcagcccatg
      181 tccgaatcga atatttccat actcctcgtc gacgacgagg cgatcatcgc cttggcgaca
      241 cagcgcgttc tcgaggacgc cgggtacgcc gtcatcaccg cgcactccgg gcgggaggcg
      301 gttgcagtcg ctacggccga cgacgcggca atcgacctca tcctcatgga cgtcgacctg
      361 ggcgacggca tggacggcac cgcgaccgcg cgggaaatcc tcgcacgaca cgaggtcccc
      421 gtgctgttcc tgtcgtccca taccgaaaag gagatcgtac gccgcaccga ggagatttcc
      481 tcctatggct acgtggtgaa aaactcgggc gacgtggtgc tgctggcctc gatcaagatg
      541 gcgctgcggc tgcatgcggc caaccggaaa ctcgagcacc agtccgagga actcgaggcc
      601 gccaacgagg agctcaacgc gaccatcgag gaactggagc aggcgaacga ggaattacag
      661 cgctctgaga gcgacctcat ggaacgcgaa cgggtgcttc acgagagcga gcttctcctg
      721 gaccacacgg gtgagatggc cagggtcggc ggatggcaac tcgttcccgc gacgctcgag
      781 gttacctgga ccagggaaac ctatcgaata cacgaggtgc ccatcggcca gcggccctcg
      841 ctcgaggagg cgatcaattt cttccatccg gacgaccgcg gcaggctttc ggaggcgatc
      901 gcgcgcgcca tcgagcacgg cgagccctac gacatggaga tgcggttcat caccgcgaag
      961 ggcaaccgcc tgtggacccg cacccgctgc attcccgagg tggagcacgg aaaaaccgtg
     1021 ctgctgcgcg gaatcttcca ggatatcacc gaacgcaaac aaaacgagct ggcgatgcgc
     1081 gaaagcgaag agcactatcg cgccatcgtg gagaacacga gcgattttat catgcgctac
     1141 gaccgcgagg ggcgccacct cttcggcaac cgcgcggcgc tcgaggtgac cggcgtcacg
     1201 ctcgacgaat acgtcgggaa aacgcaccgc gagatgggct tcccggagga tctgtgcgcc
     1261 ctctgggagg agggcatcca cgccgtcttc gagaccgccg agccgagagc ggtcaatttc
     1321 gacgtcgcgc tccccggcgg gaccatgtcg ctcgagctca agctctttcc ggagttcgga
     1381 gcggacggct cggtcgcgag cgtcatcgga gtctcgcgcg acatgagcga acacgtccgc
     1441 acggccgacg cgctccatga gcgcatcaag gagctcgact gcctctacgg cgtggccgac
     1501 atcatcgagt ctccgggagc ggcgctcgag gacgtcctcc gtgggatcgc cgcgctcatt
     1561 ccgcccgccc tgagccgccc cgaggcgtac ggcgcgcgca tcgtggcggg cgaacgggac
     1621 tacgcctcgc cgggctttac cgcgcgcacg ccgttgtgcg cgatcgacat caccgaacac
     1681 ggcgcgccct acgggaccat cgaggtgcac ggcgacgccg cacatccgct cctccccgaa
     1741 gagacgcgcc tcatcgaagc ggtcgccgag cgcaccagcc ggatgatcga gcgcaccatc
     1801 gccgaggggc gtctccggga aagcgacgag ctcatccggg cgctccgaga cagcagactg
     1861 acgctcatgt acacccacga cctcgagggg aatttcaccg acgccgacga cggcctgctc
     1921 gagctcctcg gctatcgccg cgaggagata cgatcgatca acttcgcctc gctcatcggc
     1981 gacgaacagc tcgagaaggc tcttcgcatt ctgcaggacc tcatttcgac cggcaggcaa
     2041 tcggcgcccg ccgaatatct cctgcacgcg agggacgggc gcgaaataac gatcgagacg
     2101 atcggcttcc cgatataccg gaacggccgg ctctccgcga taatcggcat cgcgcgcgac
     2161 attaccgagc aaaagagcat ggaggagaaa ctacgatcaa gcgaggcgaa gttcagatcg
     2221 ttatttgaaa acattacgat aggcgtggcc attcacgaga tcgtcgttga cgacaacaat
     2281 aatcccatag atttcacatg gctcgactgc aatccagcct atgagaacat gacgcgcctg
     2341 catcgatccg agatcgtcgg caagcgtgga ctggatatta tcccatccct cgaacataca
     2401 tggattagca cctatggcag agtcgcccta agcggtgagc cggtcatgat aaccgaccac
     2461 tcggaatacc ttgaccgata ctgggaggta tacgcctttt caccgaaact tggatttttc
     2521 gccgtggcca tgaaagacat caccgagcac aagcgcatgg aggaggagct ccgggcatcg
     2581 accgaccgcc tcgttcgggc cgaattcgca tcacattcag ggaactggga attccacttg
     2641 gccaccggat cggttttcgc atcggagggc gcgcgcgccg tatacggatt atggaatgag
     2701 aattggacca tcagcgaggt gcagaaggtc cccctccccg aataccgcgc cctgctcgat
     2761 gaggcgatgg gatcgctcat ggacggcacg cgaccgtacg acgtggagtt ccgcatcagg
     2821 cgcccgtcgg acggcgaggt gcgcgacatc cactcgatcg ccgagtacga ccgcgagcga
     2881 aacgtcgtct tcggtgtgat ccgggacgtg acggagcaaa agcgagcgga gcgggcggcc
     2941 gccgcgaatg aggagcggta ccggatgctc ttcgagcacg atctcacggg cgacttcgtc
     3001 ttcgacgtaa cccgggaacg gatcgtcgat tgcaacgatg cgttcgtcgc gctgctgggg
     3061 ttcactgacc gcgctgcggt gcaaaacggc gccgagggtt tccacgcgct cgacctgccc
     3121 gagttgcgcg cctccttcga cgcgctcagg acctcccggt tcatcagcca tcacgaggta
     3181 cgcatgcacc ggcgggacgg cgccgaggtg cacctggacc agaaccttgt gggaatattc
     3241 gaccaggcgg gcacgctgtc cctggtgctg ggatactttt acgacatcac cgcgcgcaaa
     3301 cactacgagg ccaagctgcg gcagtccctc gaggacaaga acatgctcat gcgcgagctg
     3361 caacatcgag taaaaaacaa cctcaatctc gtctcggcgc tcctggggtt ggagatgggc
     3421 aacctcgtcg accagggcgc gcgcaacata ttctcgaaca tgcaaaaccg catcgcctcg
     3481 atggcgaagc tctacgagca gctccacggc gccgacagca ctgagcgcgt ttcaatgaag
     3541 gagtacgtcg cgtccgtgtc attgtcgctc tcctccgcgc tcacgggcgg catgcgcggg
     3601 atccgcattc gcgcaacggc ggacgacatc gtgctggaca tgcgccaggc ggtgccgctg
     3661 gggatcatca tgaacgagct ggcgaccaac gccatcaaac acgcctatcc ctgggacgcc
     3721 ccgggagaga ttcgcataac gctcacccgc gaagacggcc acgccgcgct cacggtggcc
     3781 gacgacgggc ggggcctccc gccggggttt tccatcgaag acgcgaagag catggggatg
     3841 cagctcgtgg gcatcctcgc gcggcagatc agggggaccg tgaccgtgga gagcggaccg
     3901 ggaaccacgg tgcgggtttt gtttccgtta cctcatccgc ttcgatagac tcaacgtcca
     3961 cctcaccccc ggcccctctc ccataggaga ggggggagca tcaggcaatg agctcgttcc
     4021 caaggagccc tctcctacgg gagagggcgg cccgaagggc gggtgaggtc tttttcactt
     4081 gacgatacgc gaagtgatgt gctatatttg taaccacctg cccgcgaaac catttccggg
     4141 ttaatgcaac gaccaagttg ttccaaatca tgcacggcga aaacacatca cccgtctttt
     4201 atgttggcat cggcgcctcc gcgggtgggc tcgaggcgct cgaaagcttc ttctccgcca
     4261 tgccggccga cagcggtatg gccttcgtgg tgatccagca cctctctccc gactacaaga
     4321 gcctcatggt cgagctcctc tccaagcgga cggccatgcc cgtgcgccgg gcggaggaag
     4381 gcatgctggt ggaacccaac accgtgtacc tcattccgcc caagaaggaa ctcacgatct
     4441 tccacggcaa gctcatcctg cgcgaacagg accacaaccg ggggatcaac cttcccatcg
     4501 atgtgttcct gcgctcgctg gccgatgacc aagccgagaa ggcgatcggc atcatcctct
     4561 cgggaaccgg cagcgacggc gtgcgcggca tccgcgccat caaggagacc ggcgggatga
     4621 tcatggtgca gagcgaggag agcgcgaagt tcgacggcat gccccgttcg gcgatatcga
     4681 cgggccttgc cgacgtagtg ctggcccccg acgagatgcc cgggcgcctg ctgagtttca
     4741 tgaaatgccc gtacgccgcg gcggagcgtc cgcaagcgct cctctccgac gaggacggca
     4801 tcacccgcat cttcgccctc ctgagggacc agatgaaggt cgatttcacc ttttacaagc
     4861 caagcaccgt gctgcggcgc attgaacggc gcatgaccgt gaaccagatc caggaccttc
     4921 gcgactacgt gcgcttcctc gagctgcggc ccggcgaggt gagcaccctg caccgcgagc
     4981 tgctcattgg cgttacgagc tttttccggg accgcgaggt gttcgatgag ctgtgcgaaa
     5041 aacagctccc ggcgctcctc gacagcattg accagcgcga ggtgcgattc tgggtggccg
     5101 gctgctccag cggggaggag gcctactcgc tggccatcct ggcgcgcgag tacctggacc
     5161 gctcccgccg caccctgaac gtcaaggtgt tcgccacgga catcgaccgc gacgcgctgg
     5221 tccacgcggg aaacgggctg tacgccgaaa gcatcgccgc cgacctcccg ccgggcgtgc
     5281 tgacgcgcca cttcacgcac cgtgacgacc gctaccaggt gaaccgcacc atccgcgaga
     5341 tggtggtctt cgcgcagcac aatctcatca aggacccgcc gttcaccaac attgacctcc
     5401 tgagctgccg caatctgctc atctacctgc agcccgtcct gcagcaaaag gtgatggagt
     5461 ttttcaactt ctcgctcaac ccgcagggga tcctgcttct gggcaccagt gaaacgccgg
     5521 gggacatgtc cgacttcttc gaacccctgc atcacaagca caagatatac cgctccaagg
     5581 ggaagaagcg gccgcccctc gagcgccatt tcgccccgca cccgacgaaa accgccggcg
     5641 gccggtacgc cgcgcgcctc ggccggggcg cgcaggcgct cctccagcgc gaggaggagc
     5701 gcgtcatgga gcgcctgctg caggccatgt ccgacaacgc cgacagcatg gccgccgtgg
     5761 tgaacgagcg cctggagctt ttgcatatcg cgggcagccc cgacgggtac ctccgccttc
     5821 cctcggggaa gctccataat gacatcgccc gcctctcggt gcgcgagatc gccatcccgc
     5881 tcaccacggg gctgcagaag gtgttcgcga ccggcgaggc tgtccgctat acgaacatcc
     5941 gcgttacccg cggcggcgcc gagcggatgg cgcacatgca catccgcctc ctccccggcc
     6001 gcacgggaca ggagccgctg gccggggtgt tcctgcagga catgcccgtc gcgcggcccg
     6061 aacccgcgcc cggcgcgggc cacgagttcg acatcagccg cgaggccgag cagcggatcg
     6121 gcgaccttga gcaggagctg cagttcacca aggagaacct gcaggccacc atcgaggagc
     6181 tggaaacctc aaacgaggag ctccaggcca ccaatgagga gctccttgcc tccaacgagg
     6241 agctgcagag caccaacgag gaactgcagt cggtgaacga ggagctgcac accgtgaacg
     6301 ccgagtacca gatcaagatc ctcgagctca ccgagatgac caacgacctg gacaacctca
     6361 tagcggccac ggggatcgcc acgctctttc tggacgagaa cctcgacatc cgcaagttca
     6421 cccccgagct caagcgaatc ttccgaattc tcgagggcga tgtggggcgg cccgtaacgc
     6481 acctcacgca tgccatcgac ggcgccgacc ccatcgaggc cgtgcggcgc gttacggaga
     6541 ccggcgcgcc cgaggagcgc gaggttcgga ccgccgacgg ttcgtggttc ctcatgcgcg
     6601 cgctccccta ctacgtgaac gagtcgtcgg tgtcgggcgt ggtgctcacc ttcacggaca
     6661 tcggaaccct caagcgggcg gagcaggccg tctcggagag cgaatctcgt taccgcgagc
     6721 tcttcgagag catgcgcgac gggtacgccc tccacgagat catcaccgac gatgccggcg
     6781 ttccggcgga ctaccgcttc gtggcggtga accccgcctt cgagcgcctc accgggctcg
     6841 cggcgagcga catcatcggc aagcgggtgc tggaggccat tccgggaacc gagcggcact
     6901 ggatcgagac ctatggacgc gtggcgctca ccggcgagcc gctgtcattc gaaagctaca
     6961 gcggcgccat cgacaagcat ttcgacgtgt acgccttccg ccccgcggag ggcatgttcg
     7021 cctgcgtgtt ccgggacatc accacgcgca cgcgcgcgct cgatcggctg cgcaaggtga
     7081 ccgatgtcct gaacgccacc caggagctca cgaaaaccgg cggctgggaa tgggatgtcg
     7141 agaaacggga gatgttctgg accgaagagg tgtaccgcat ccacagaatt gatcccggcg
     7201 cgatcccgcc cggctccacc gagcatatcg accggagcgt ggagtgctat gatgaagccg
     7261 accgcccggt catcgcggag gcctttcgcc gctgcgtcga ggagggcgtt gcgtacgacc
     7321 gcacgttttt cttcaccgac acgcaggggc ggcgaaagcg catccgcacc gccgcgcatc
     7381 cggtggtgag cgacgggcgc atcgtgcggg ttattggtaa catcgtcgag ctgaacgatt
     7441 caaatacata ctgcaacgac cgtactgaaa ccggataagc gccatgcgaa gttttaatcc
     7501 gtcgaggggc taatgggaaa tgataacgat cgcgcggatc tgagaaggcg cgccgaggag
     7561 tcgttgcgcg ccagcccgcc ggaggcgcga ccccgcaccc ccggcgaagc cgaccagatg
     7621 attcaggagc tccaggtcca ccagatagag ctggagatcc agaacgatga gctgagaaag
     7681 acccaggaaa acctggtggc ctcgcgcgac aggttttccc ggctgttcaa caacgcgccc
     7741 gccggctatc tcgtactgga cgagtcgggg ctgattgtgg aggccaacga gacgttttgc
     7801 cgcatggcgg ccgtgcggcg cgaccgggcg ctgggccgcg ggatcgccga tttcattgcc
     7861 ggggccgacc gccacggctt cctttcccgc tatcgcgcct tttacaacaa tccatcggga
     7921 aaaacactcg aggcccagct tgcctccggg gattcggtag cctgggtgcg catggaggga
     7981 tcgcgtattt ccgcgatcga cgccgcggac cccgaggcgc gccagctcct gttatccatg
     8041 agcgacatca cccagcagaa gcgcgccgag gccgcgctcg cggaacgcga gagatatctt
     8101 caaaaaatcc tgcagacctc cgccgacggc ttctgggtgc tggacatgaa cgggcgcttc
     8161 accgacgtga acgacgccta ctgccgcatg tccggttacg gccgggagga gtttctcgcg
     8221 ctcgccatcc acgacatcga tgccatcgag ggtccggagg agaccgcgaa gcggatcggt
     8281 cgcatcatcg aaaagggaac cgacatcttt gaaacgcggc accgacgcaa ggacggcagc
     8341 gcgttcgacg tcgaagtgtc cgttgcgtac tgggagtcgg ggggcggcca gttcgtgtgc
     8401 ttttgccgcg acatcaccga gcgaaagcga aacgagctgc agctgcgcga gcgcctcaaa
     8461 gagatgcgct gccactacgc cttctccgac cttcttgact cgcacccgcc gatcgacgcg
     8521 gacatgctcc ggcgcatcgc ggagctcatc ccgcccgcca tgctgcatcc ggagatagcc
     8581 gccgcgcgca taacggtgga cggcatcgcg gccgaatcgg gcgactgccc cgatacgccg
     8641 tgggtcatcg ccaccgacat catgatacac gaccggcgcg cgggcgcggt caccgtgtgc
     8701 taccgcgaag atagcccctc ccccatctcc gactcggcgc cattcctgcc ggaggagcgc
     8761 cagctcattg acgatatcgc cgagcgcctg ggacggcaca tcgagaaggt gcgcgcggag
     8821 gaggcgcttc gacgacggat taacgagaac gacgccctcc tcatggcctc gcgcgcggtg
     8881 ctggaacagc gcgatttcac cgcaacggcc cgaactattt tcgatatcgg caaaaacatc
     8941 accggggcca cggcgggcta tgtcgcgctc ctgtccgcga ccggcgagga gaacgaggtc
     9001 ctcttcctcg acgcgggggg ccggccctgc acggtggacc cgtacctgcc catgcccatc
     9061 cgtggtttgc gcgcggagtc gtatcggcac cgtaaaacag tgttcgacaa cgacttccac
     9121 aacagcgagt ggatgtcgtt catgcccgcg ggacacgtgc ggctggacaa tgtgatgttc
     9181 gcgccgctga tcgtcgaggg gacggcggtg ggcatcattg gcctggccaa caaggacggc
     9241 ggctttaccg acgaggacgc ccggatcgcg accgccctcg gggacatcgc cgccatcgcc
     9301 ctcatgaaca gccggaacct cgaaaaactg aaggcctcca tcggcgaacg cgacgtcctc
     9361 atgcgcgagc tgcagcatcg ggtgaaaaac aacatgaaca tcatcacctc gctgctctcg
     9421 ctggagatgc ccaacctggc cgacgagaac gcgcgccgca tcttcaaaaa catgcagggc
     9481 cgcatcgcct cgatggcgaa gctttaccag cagctttaca gcaccggcag ctacagccgg
     9541 gtgtcaatga acgactacat ggacgccatt acagcatcca taaactcctc actgggtggc
     9601 ggcgcccgcg gcgtccggat ctccgcggca tccgacgaca tcacgctaga gatgaaccag
     9661 gcggtcccgc tgggcctggt gatgaccgag ctggcgacca acgccctcaa gtacgcctac
     9721 ccgcccgacg cccccggcga gattcgcata acgctttccc gcgcgggcga cgccgccgtg
     9781 ctcacggtat ccgacgacgg gccgggcctc ccgccggggt ttagcatcca aaacgcgaag
     9841 agcatgggca tgcagctggt ggacattctc gcgcgccaga tcaagggaac ggtgtccgta
     9901 gaaagcggac cgggaaccac ggtgcgggtg acgtttccca cctcatccgc ttcgacaggc
     9961 tcagcgctca cctcaccccc ggcccctctc ccataggaga ggggggagta tccggcaatt
    10021 atctttttcc caaggagccc tctcctacgg gagagggcgc ggcgacggtc cccgtgctct
    10081 gtcgaggggg agccggggtg aggtcacgac agccgctcga aagaaatcga ttcgtacagc
    10141 tcatcaagcg cccggtccac atctgcatgc acggcctcat tccgcaggcg aacaatccgt
    10201 ataccccgat gagataaaat cttgtcacgg tgcgcatcat acgtcacctg ctgctcgttg
    10261 tcgtgcactg atccgtcaat ctcaacgcaa agtttcaatt cctcgcaata aaaatcgaca
    10321 atacatccgt cgattacctg ctgacgacga aacttcaggc tcttgaattt tcgattgcgc
    10381 acgcgacccc agaacacctc ttccgccgca gtctgctcct tgcgcagctc acgcgcacgc
    10441 tcagtctttc tcccggcgcc cgattgtcct ttgattattc cgctgtttcc cataacgggt
    10501 ttacctcatc tcacctcacc cccagcccct ctcccatagg agagggggga gtgcaagtaa
    10561 tctcctatgc tcccccttct cctatgggag aagggggtcg gggggatgag gtaacccact
    10621 ctcgcgcgcg acgggcaagt tcgtgggagt tttcgatgtc gagtttcttg cggatgtgct
    10681 cccggtgggc ctcgacggtc ttcacgctga tgttgagctt cttcgcgatc tgcgcggtct
    10741 tgagctcgcg gcccatgagc tggaacacct cgagctcgcg atcggtgagg cgcgcgatgg
    10801 gatcgccggc gttgccggga ggctcctcgg cgatgcgtcc caggatgcgc gagatcacct
    10861 cgtcgctgaa gaacttgttc ccgtccagga ccttgcgaat ggccgaaatg agcacctcgg
    10921 ccggccggtc cttcatcaca tagccgttcg cgccggcgcg caacgcccgc tccgcgtaca
    10981 ccgactcgtc atgcatggaa tacacgatcg cgggcaagcg cgggttccgc gcacggacgg
    11041 ccttgatgag gtcgatcccg ctggagctct tgagcgagat gtccaccacc accaggtcgg
    11101 gggcgagcga ttcgatcttt ttcatggcgt cgttgatgtc gcccgcatcg ccgcatacgc
    11161 gcagatcgcc ctcctggtct atcacctgcg ccaggccccg gcagatcagg ggatgatcgt
    11221 ccacgatgag aatgcgtttt gccgtccgtg ccgtttccgc tgttttcatg caatcgtccg
    11281 ctctctgtgt gtccgtcgca ggggggccgc gcaccgcacg ctccggccat ggagcgtcac
    11341 tcgatcgccg agagcggaaa ggtgcagtgg accgtcgccc cgcccgactc gttccggtag
    11401 aagcgcgccg tacccgagag cgattccatg cggtacttca tgatgcgaag accgagcccc
    11461 gtggcggcat cgagcgtctc cggcggcccc gcgccgttgt cctcgataac aagcgtaccg
    11521 ttttcgccct ccgatgtcaa ccgaattgcg atccgcttcg gattcccgtg cctgatggcg
    11581 ttgttcaccg cctcgcgaac gatgaagtac agctgcgccg acgcggtctt gtccgtgatc
    11641 gaaaggcccg ggtccatttc gaactcgcac tcgacggaaa agaccgtgcg cacgccctcg
    11701 acgaagtccc ggaccgcgta gaccatgtcg ttttcgacga gcgtgggcgg atagagctcg
    11761 cgcgagaggc cccgcgtccg ggagatgacc tcgtagacgt gccgcacgac ctcctcggcg
    11821 tcgcccgcaa gcgggtgcgc tgagtccttg agccgctttg cgagcgatgc gcccaggaag
    11881 gcgatccccg tgagctcctg gccgatgccg tcgtgcaggt cgcgccccag acgctcctgt
    11941 tcgatgtcga tgatgcggat gatctcgttt tgcagccgct tgcgctcggt gatgtcgcgg
    12001 aacgccgcgt gcaccaccgt ccggctcccc aggcggatcc gcgtggcgta cacctcgaca
    12061 atgaatgacg aacccccgcg ccgaaccagc tcccactcga accggaagct gccctcctcc
    12121 gcgagccgac gcgaaaccag gtcgcccttc gcctccgaga agatgccgtc gggctggacc
    12181 tcgggcgagc agtcgtacag gcttttaccg accaaatcgc gcgcgccgcc gtattcgagc
    12241 agctgcgccg ccgcgccgtt gcaatccacg accgcgaacc ggtccaggag cgcctgcgcg
    12301 tcggaggagt cctcgaagag gcatcggaac ttctcctcgc tggcgcgcaa ctcctgctcc
    12361 gcccgaacga tggtggtgat gtcgtgcgca aagagcgcga tattgaccac gaagcgattc
    12421 gcgtcaacga tggggtacag cgtcacgtcg aaatggcgat tgtcacggcg cagcatgaaa
    12481 ttgaccggtc ggcccgtcgc gaccaccgcc tccacgcgcg aaaccgcgcc ccgcacgatc
    12541 ggcccctgcg gggcctccgg gaccgcgcgc tcgatgagga ccgagtagtc gtccccgaag
    12601 tcgagccgga aggcgtcgtt ctccatgagg gtcacgccgt ccacgtcgag gagcgcggcg
    12661 ctgtcggtag tcgcgttcag aagcgaaaac aggatccgct gattgttctg cagccgctcc
    12721 tcggcctgct tgcgctgggt gatgtcggtg aacatggcga aggacccgtg aaacgagccg
    12781 tcgtcgcccg caagcgccgt ggccgaaaca aggcaccaga gcgtcgatcc gtcggcgcgg
    12841 cgaaagcgct gctcgtaccg gccgccctcg ccgcgcgcgc gcccctccat gcgtcgctca
    12901 tgttccgcca ggtcctcggg aaacatgaaa tcggttacgc gttttcccac gatatccacc
    12961 gccgggcgtc ccagcatctc ggccatttgc cggttcacga acgtcgtcac tcgatcgccg
    13021 tccatggacc acacgccctc cgcggcggtt tccacgatgc gccggtatcg ctcctcgctc
    13081 ttgcgtatcg cctcctgcgc gcgccgctct atcgttacgt cgcgcgcgaa gatggcgatc
    13141 tgcgccgtgg cgccccgctc gtcgcggatc ggatagatgt ggtgctcgag gagcacgccg
    13201 ccgcgctcgt cctcgaaggt caccgccatg cccgtcgcaa gcgcgcgatc gagatggggc
    13261 tttcgggagg cggcaaggtc cggcggaacc aaatcgagga tgctccggcc gatggcctcg
    13321 tcggcccgca tccccaccgc ctgggccgcc acgatattga ggcccagaat cacgtagtcc
    13381 cgcccgatga gaaacgcccg gtcccggctc gcgtcaagga gcgcgcgggc cgtgcgctcg
    13441 ctctcgacca gcctgcgctc cagcgcatgc ttgttgactg ccgtgtcgat cgtcgagaag
    13501 aggtgcaggc gctccaccgg cttcaggaca tacccgtagg gcgccgtatc gcgcgcccgc
    13561 gcgacggtgc ccatgtcggc gttggcggtc atgtacacga cgggcacggc gaagcgctcc
    13621 cggatctcgc gcgcggcctc caccccgtcc atcgccccgg ccaggacaat gtccatgaga
    13681 atgaggtcgg gcgcaagccg cccggccagg tccacggcct cctcgccccg gtcggcggtc
    13741 cccaccaccg catacccgaa acgcgtcagc atctcgccga tgccgcgcgc aacgacgagc
    13801 tcgtcctcga cgatgagaat gcgcgcgctc atcgaccaaa cctcaccgtg aaccgggtcc
    13861 cgttcgagcg gtcgatcgcg agctcgccgc gaagctgctt gaccagaagg cccaccagta
    13921 tgaacccgag agcgttgccc gtcccgtctt cggcgccctc gggaagacca atgccgttgt
    13981 ccgcaaccgt caaacagacc cggtcctcgg cctcctcccg cagggacacg aaaagctccg
    14041 gctcgcccgc ccagccgtcg gggaaggcgt gcttgcaggc gttcaccaga agctcgttga
    14101 ccacgaggcc gcacggcacc gcccgctcga tgttgagata gacgggatgg gcgtccaccc
    14161 gcatcgcggg gacccgtttc ccccgcgggc aatacatggc catgctctgc acgatatcgc
    14221 ccagatactc cgcgaaatcg atccggccca ggtcggccga ctggtacagc ttctcgtgca
    14281 ccagcgccat cgacttgatg cggccctgcg cggtggcgaa ttgcgcgatc acctcgcggg
    14341 cctcctgccg gttcgcctgc aagtccagca ggctcatgat gagctggaag ttgttcttca
    14401 cgcgatggtg gatctctttg aggagcgtct ccttttcgtt aagggaatcg acgatcgcgt
    14461 cctgggcgcg cttgagatcg gtaatgtcct ggttcaccac cagcgccccg aggaccgcgc
    14521 cgctgtcgtc gcggaccggc gcggtgaagt tgagcaccgt gcgcgtcacg ccgtcgaagg
    14581 cgtcgatctc aagaagttcg ttcgcgatgg tcacgccctc gttgatggtg tgcgcgagcg
    14641 cccagtcgtc cggggcgatc tcctcgcgtg acggcaggcg acgcgccttg aacacccggt
    14701 actcgtcctg tcc
//
LOCUS       DAORHF010000075        14689 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3162_length_14689_cov_5.875085, whole genome shotgun sequence.
ACCESSION   DAORHF010000075 DAORHF010000000
VERSION     DAORHF010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14689
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3162_length_14689_cov_5.875085"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PKJ16_08105"
     CDS             complement(<1..1201)
                     /locus_tag="PKJ16_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46988.1"
                     /translation="MDVLIPKDRMSMIERGARAALFAFSLAFFAAHVGNSRFGFWSAS
                     PWPSDLVMLFLLFNALHQGAALIKNREGVLPTRILLSAACILAMIFSFFVPLIVFGVW
                     AAFDRNEDRKWVNDLLVGAFVSGTIGAILVLIVHPAENVIPYYSAIAAYSAAGVIAAA
                     VLRFRFRGRRADGVRSVPDSVAVPSRKIEGEIDALADGCRRLGESEVRIGQGMREGEW
                     QQVPDASRFCETPYSRSAWRLHGFNGWNDVIPPFDERGFLESVMMLPRLARDREHVLW
                     AIRKIVDTFIFPASALCMVLVEQNGPVLYQNDDVVYPVSLDDFEMDNPRSLAWRVLRD
                     GADFFIEDVGNWENTGELPNIYTMPQQPQVGEDGRFPDYVVEPKDSIRHMPCGFLQRD
                     RSLLLQCL"
     gene            complement(1211..2071)
                     /locus_tag="PKJ16_08110"
     CDS             complement(1211..2071)
                     /locus_tag="PKJ16_08110"
                     /inference="COORDINATES: protein motif:HMM:NF012629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46989.1"
                     /translation="MKCARCNRAITDAVYKTPAVTRNGKTYCPRCVTMCVECRKKLVP
                     GIDAIKEFNGRPYCTACYRAMDWLTCAGCRVKIIGDYSIILRRLFCPSCAGSARCPLC
                     GCPIGKNIARDARGRVICFFCSRPPRADVAIAAAADEVRAAVSRMCGLELPAAPVAAM
                     PYDELQKHSPWNTLQAAGLYAPAEDAVLIVPYYPPEYLKAVLCHELVHAWQFRNWRED
                     YDMRTREGMAMYVEYRYANSMGFREEVAAIESMLKEKTASNYSIGLKMFLDMAREKGE
                     EAVLAYFRSL"
     gene            2335..3672
                     /locus_tag="PKJ16_08115"
     CDS             2335..3672
                     /locus_tag="PKJ16_08115"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV46990.1"
                     /translation="MKRIVIIGFGSAGYAAFTAARKTDPKAAITVIDPKARDLMHPCG
                     LPYALEGRADPARLEQDVHLDKAGVIRIRARATRIDADAKRIITDAGDTEGGVPYDAA
                     IICTGSAPVLPPVPGIERLRNRGLYTLATMDDCNRIMERIEGASDCLIIGAGAIGLEC
                     AVALRARGRAVHLAEMRDQVLPGVIDADLAAPVAEHLTLRGIAFHLGAAVGEVLGEDA
                     FAGARIGDSRITASLCIAATGFAPHTELARESGIECGPRGIVVDDSLRTALPDVYAAG
                     DCIVAWSVIDKKPVGAKLATSAYKQGTVAGANAAGGAMTYAGTAGAFVTVLGDLEIAG
                     AGYTTEAARAAGYEPVSGKISAPVLPDYIPDGHAITVKILADRATGRLLGAQAVGPAG
                     AAARINVASAAIEFGKTLDDLERVELAYCPAVSEVHDPLLRAADFARRRMPKR"
     gene            3690..4073
                     /gene="gcvH"
                     /locus_tag="PKJ16_08120"
     CDS             3690..4073
                     /gene="gcvH"
                     /locus_tag="PKJ16_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005770508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HNV46991.1"
                     /translation="MSATPDELKYSADHVWIRMEDDFIGVCGLTDHAQEQLTDIVFVE
                     LPEIDTDVRQGERLAVVESVKATTNVNAPVSGRIVEVNTALEDSPGVINADPYGDGWI
                     FKIDVKGRVEYDDLMDADQYRAQVE"
     gene            4079..4675
                     /locus_tag="PKJ16_08125"
     CDS             4079..4675
                     /locus_tag="PKJ16_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV46992.1"
                     /translation="MDRLRAQIAFIVEIDKLKQVLRRNYLADGSRRENDAEHSWFFAT
                     AALVLREHAKEAIDIARVVSMALIHDIVEIDAGDTFIYDTAARADQHEREERAAARLF
                     GLLPDDQARDLCALWDEFEAGKTPEARYARAIDRLSAVLLNHASGGKAWREHGVPASR
                     LREVNGAIADGAPAIWEHVRGIIDDAVARGIVADADHS"
     gene            4653..5513
                     /locus_tag="PKJ16_08130"
     CDS             4653..5513
                     /locus_tag="PKJ16_08130"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HNV46993.1"
                     /translation="MRTRTIPDWIVQRVPSGERYRAVRRAVDGLGLSTVCAEARCPNT
                     GDCLCRGSAAFLILGRICARGCRYCAIETGAPIPPDPSEPDRVARAVAALSLRHAVIT
                     SVTRDDLPDGGAGAFAETITRIRERAPGCAVEVLVPDFRLSQEASMDSIIAAQPDIIN
                     HNIEVARPRYHLLRPNGDYDASLALIRRAAASGIRVKSGLMVGFGETLDDIDATLADL
                     RAAGCGMLTVGQYLRSRRECVPVARFYPPEEFEEIARRARGMGFTAVHAAPLARSSHH
                     AEVFARSFPG"
     gene            complement(5554..6912)
                     /locus_tag="PKJ16_08135"
     CDS             complement(5554..6912)
                     /locus_tag="PKJ16_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46994.1"
                     /translation="MSIEIRKEFLKILANSLNTGQINYLGERLTPGFSVQRESGFSPS
                     IPLPAQTAAETLVRYYADEDDIVRLFGILLENENARFYNAPLRIWERDRFIKLLARNK
                     WMYDPDLKYFCRDPFYEREINLLKKLRVIDLRAQPRLEKIVSDIAAAANKLSIRDLEW
                     RITIRCYDMDRDAVELVRKIIDLLLARQDLLGYSYELFTCLKELAVNATKANYKLLFD
                     RHVARPQGVGAGSDYREFMRLFKEEIEENANRRLVELARREDRYITIFFQSSPESVAI
                     WVANNASISTIEKRRLLRKIRHGELSGDSFEAASDDFAEGAGMGIGLVLSILKRHSSE
                     REPLKVVFYPDSIKIGFELRRDELSKEKERESAPATAGSDAGHVLGGQTLSTVAGDAR
                     RGATASGARKKKSAPKTSGARGPSGKKKTATKRGPSPAKKKPTARSASSSKIKKQQKK
                     KR"
     gene            complement(6960..8729)
                     /locus_tag="PKJ16_08140"
     CDS             complement(6960..8729)
                     /locus_tag="PKJ16_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46995.1"
                     /translation="MDERKYIKEVIARPATRGEREQAERLRKFSADSGEIVEMRAKLR
                     GLMDDLVDDEEKLKRQQGAIGGLHILPQYQRRAYDRSILVKSPSSAFVSLFSYFGIRR
                     EFRQKYKAAGTISWSFWSFFSGGDLFWPSMQVYRFVQKLVVIMEELREPFRRMYAQGW
                     LSRAGADVLSPLEFNLIGEMERLTADASIFNVLLDIRKPRSAVKKINPFLGYYFSLTR
                     NGVYGDRLVEALRKSQRRIVSSDGGDSRQTERAVAAVRQLLSGETDREFVVPLFECAY
                     HRTLTGEDIRAMVRLEEVDDAEYRADAHLYEAMIARKRKFQERLREAIAALTRDIELV
                     TDIGAALSRRYPVKHGALDIVEYLLYYLYHNNDETFRIKKNNLAGSAADACEVFAFAY
                     EPLIAEGVYVRVEQDKRFISLFKPDVFERQIEAIKRESRVFLECVRKGYGWPLLKKET
                     LSREEAIFMNALSTVTDAFYSMGLLLFDCLRDASLPESAAGPLGAAEIGVRAVPSADR
                     VILAAQSPAPNRYTVSMKTVRDALREAKDFCFTFSARFEWAGRDFSQTVRQRSIAEMT
                     GSLDALRERLQRMEAGDIDDIMA"
     gene            8895..10202
                     /locus_tag="PKJ16_08145"
     CDS             8895..10202
                     /locus_tag="PKJ16_08145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV46996.1"
                     /translation="MDQHPHQPTEQKTDDCRDRLGFLQGLIDAIPNPIFYKDRDGRYL
                     GCNKAFEAYLGTTRTEIVGKTVFDLSPPDLAARYHEMDERLFRDGGVQEYEASVLHDD
                     GNRHEVEFHKAVFLAPDGRPQGLVGVILDITERKRAEEALRVSEERFRIIYEQSPIGI
                     ELYDERGELLEINAACLEIFGVADPDAVRGFRLFEDPNVSASAKLRLQGGEEVRYRTP
                     FSFDLVREHGLYKTSMSGIIHIDVIITPISAADGAPPARYLVQVQDVTEAVLREENLN
                     YLSMHDQLTGLYNRAYFEMEMERVQKSRMDTAGVIVCDIDGLKAINDTRGHQAGDELI
                     RAAAGIIRKSFRDSDVVARIGGDEFVAIVPNCTPEILVTASLRIDRNIAAHNLAGSGP
                     AVAVSIGAACGRLADRRVIDIFNDADANMYADKAKRRSGRGAS"
     gene            10199..10498
                     /locus_tag="PKJ16_08150"
     CDS             10199..10498
                     /locus_tag="PKJ16_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HNV46997.1"
                     /translation="MTDGRLEASCKPTGGKLVRVSLSLAGGRIERIAITGDFFIMPES
                     GVEDIEAALVGADPGAVRARIDAVVRNRGLRLAGVSPASIAEAVRRALEGGERPA"
     gene            10495..11286
                     /locus_tag="PKJ16_08155"
     CDS             10495..11286
                     /locus_tag="PKJ16_08155"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HNV46998.1"
                     /translation="MSDTPWRGIITPPAPGPYNMGLDYALLESVAGGSAPVVRCYRWA
                     PCAITIGYFQDPEREVDLAAAARDGVAVIRRVTGGGAVFHDCELTYSIALPERASPEL
                     RSIAGSYDMICGAVARAISRLGIDASFAPVNDIIVGGRKISGSAQTRCGGAILQHGTV
                     LIDADRARMSAYLRADPEKSRERDAAGPAERVVPLRDLIGDRALAPETYSTLCDAIIG
                     SFAGTFGRAIEPSSPTGPESQRAAALARDLFGDDDWNARRIRPPL"
     gene            11467..11742
                     /locus_tag="PKJ16_08160"
     CDS             11467..11742
                     /locus_tag="PKJ16_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV46999.1"
                     /translation="MSHHDKDHVPMPLNTPAFSCTGCGAVALNADSLCQPAGRVRKSD
                     WCGTESIDMPPFCLNDRHTLRYRCKKCGRISIDAPLLCEPEKVPVKE"
     gene            complement(11751..13097)
                     /locus_tag="PKJ16_08165"
     CDS             complement(11751..13097)
                     /locus_tag="PKJ16_08165"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47000.1"
                     /translation="MATLKDIVFVSRDRSRAGSFEDTFTGEMLASERLRATIIAAFFL
                     LGGLFIGAATLLFPQGFPREMFHVRYGLPVQAWYIIFFAVSGVYEIFVRWMFGYFMRT
                     DRPFPRLMRYGNAIVEVSGPTVIVFIGSILYAEAPSLALNAPIVFLYFIFIALSSFRL
                     SIALCLFTGALAAVEYLALALWLVGLPHAEAVNPLLLSQAQHIGRAALLLVAGIVTGF
                     VALQVRRRIFRAHQAVQERERVMDTFGQHVSPAVVNKLLEQKGEFSSEMRHVCMMFLD
                     IRNFTAFSAGRDPEYVIGYLNTLFDFMIDIVNAHNGIINKFLGDGFMAVFGAPLSDGR
                     DSLNAVRAGRAIIERLAEEVRAGRVPATTVGIGLHAGRAVTGNVGSSVRKEYTIIGDV
                     VNLASRIEQLNKQFGSQMLVSSAVREAVAGEGFVGEDLGPVEVKGLREPIRVYRLA"
     gene            complement(13108..>14689)
                     /locus_tag="PKJ16_08170"
     CDS             complement(13108..>14689)
                     /locus_tag="PKJ16_08170"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47001.1"
                     /translation="LPDLHGQRSPYVEKIAALPADSFEKELPAVLAAGHNRFDGYRAA
                     LERIAASIPRATPDPARLMVARVEDLAKALRYRANKIRNLPSDDFRASLAVYLNKTDD
                     AFRPFATAMRDHLKRSRAEGFALKGEVLDYIAPMQPAGVRIYRGGRGAHFVSYMRGDG
                     AGRIIHEVGFTYESYRAYLHPAALKMVIMLVATLMVVMVGFRFFFLGALINPLETLLH
                     GLQEVRQGNLDIRIPIKVEDEIGFLTYNFNRMVRTIRSAKKRLDNHAENLEKIVDRRT
                     AELKVARDALWGEMELAKRIQTILLPEHPSIPGYEIAAHMMPASEVGGDYYDVINDDE
                     VDWVIIGDVSGHGVPAGLVMMMVQTSIQVILRGKPLVKPSVLLRVINRAITYNIKKLD
                     EDKYMTITAFAFLKGGRVLFSGLHQDIYVFRAATGTVDVIETNGMWLGFMEDLGRLNR
                     DFEFTLHVGDTLLLYTDGITEAMSPHGELFSNERLKQVFESLGGRDPEGIKRGILDAA
                     NEYTMKDDVTLFVMKRRA"
BASE COUNT         2667 a         4926 c         4609 g         2487 t
ORIGIN      
        1 taaggcactg gagcaggagc gagcgatccc tttgcaaaaa tccgcacggc atatgccgta
       61 tggaatcttt cggttcgacg acgtaatcgg ggaagcggcc gtcctcgccg acctgtggct
      121 gttggggcat cgtgtagatg ttagggagtt cgccggtatt ctcccaattc ccaacgtctt
      181 cgataaagaa gtccgcgccg tcccgaagta cgcgccaggc aaggcttcgg ggattatcca
      241 tctcgaaatc atcgagtgag acgggataga cgacatcatc attctggtaa agaaccgggc
      301 cgttttgttc cacaaggacc atgcacaagg cggaagcggg gaaaatgaat gtgtcgacga
      361 tcttgcgtat cgcccacagc acatgttccc tgtcacgcgc caggcgaggc agcatcatga
      421 cgctttcgag gaaaccacgt tcgtcgaacg gagggataac atcgttccat ccgttaaaac
      481 cgtgcaaacg ccacgcgctc cgggaatacg gcgtttcaca gaaacgggaa gcgtcgggga
      541 cctgttgcca ttccccttct cgcatacctt ggccgatgcg cacctccgac tccccgagcc
      601 gccggcaccc atccgcaagc gcgtcaatct ctccctcgat tttccgggac ggaacagcga
      661 cactatcggg cacagaacgg acgccatccg ctcgcctgcc gcgaaaccgg aaccgcaaga
      721 ctgccgcagc aataacgcca gccgccgaat acgcggcgat tgctgaataa taaggaatta
      781 cattctcggc agggtgtacg atgaggacaa gaatcgcgcc aatcgtcccc gagacaaacg
      841 ccccgacaag aaggtcgttc acccatttcc ggtcctcgtt ccgatcaaaa gccgcccaaa
      901 ccccgaacac gatcagtgga acaaagaaag agaagatcat cgccagaatg cacgccgccg
      961 ataaaagaat gcgtgtgggg agcacgcctt ctctattctt tatcagcgcc gccccttggt
     1021 gcagagcatt gaagagcaga aaaagcatga cgagatcgga tggccatggc gacgcgctcc
     1081 agaaaccgaa ccggctgttg ccgacatgag cggcgaaaaa cgccagtgaa aaagcgaaca
     1141 gagcggcccg cgctccacgc tcgatcatag acatacggtc cttaggaatt agtacgtcca
     1201 tgcagcgcct tcacagcgac ctgaaatacg caagcaccgc ctcctcgccc ttctcgcgcg
     1261 ccatatccag aaacatcttc aggccaatcg aataattcga agccgttttc tccttgagca
     1321 tggattcgat ggccgcgacc tcctcgcgaa agcccatgct gttcgcgtat cggtattcca
     1381 catacatggc catgccctcc cgggtgcgca tgtcgtaatc ctccctccag ttgcgaaact
     1441 gccaggcgtg gaccagctca tggcacagga ccgccttcag gtattcgggc gggtaatagg
     1501 ggacgatgag aaccgcatcc tcggcgggcg cgtagaggcc ggccgcctgc agggtgttcc
     1561 atgggctgtg tttctgcagt tcgtcatagg gcatcgcagc gacgggcgca gcgggaagct
     1621 ccagcccgca catccgcgaa acggcggcgc gcacctcgtc cgctgccgcc gcgatcgcaa
     1681 cgtccgcacg cggcggccgg ctgcagaaaa agcagatgac gcgcccccgc gcatcgcgcg
     1741 cgatgttttt tccaatggga caaccgcaca gcgggcaccg ggccgaaccc gcgcacgagg
     1801 gacagaacaa ccgtcgaagg attatggagt aatcgccgat gatcttcacg cggcaccccg
     1861 cgcaggtgag ccagtccatg gcgcggtagc aggccgtgca gtacggtcgc ccgttaaact
     1921 ccttgatagc gtcaatcccg ggcaccagct ttttccggca ctcgacgcac atcgttacgc
     1981 agcgcgggca gtaggtcttg ccgtttcgcg tcacggccgg agtcttgtac acggcgtcgg
     2041 tgatggcgcg gttgcagcga gcgcacttca tgcgatactc ccatcgattt attatcgcgt
     2101 gcctggcatc ggccgcaaaa atccaacgct ccgccgacaa ccatcacgcc atacacgatt
     2161 cggaaaatat tcaagaacaa attcaatacc gcggtagccg cagcacgggc atgatccaca
     2221 aacgcacgcg tcgaaaaacc cctcacgtcg ttcatgcgct tttttgtatt gacgcccccg
     2281 ttttttccgt gccgatgggt acatcgcacc gcaattcgcc gccggggagc gcacgtgaaa
     2341 cgaatcgtca tcatcggctt tgggtcggcg ggatatgccg cgttcaccgc ggcgcgcaag
     2401 accgacccga aggcagccat caccgtcatc gacccgaagg cgcgcgatct catgcacccg
     2461 tgcgggctcc cctatgcgct cgagggccgg gcggacccgg cgcggctcga gcaggacgtc
     2521 catctggaca aggcgggggt gatccgcata cgcgcgcggg cgacgcgcat cgacgcggac
     2581 gcgaagcgaa tcatcactga cgcaggcgac acggagggcg gcgtccctta cgacgccgcg
     2641 atcatctgca ccgggagcgc gccggtcctc ccgccggttc ccggcatcga gcgcctgcgc
     2701 aatcgcggtc tttacacgct cgccacgatg gacgattgca accgaatcat ggaacgcatt
     2761 gaaggggcat cggactgcct gatcatcggc gcgggcgcga tcggcctgga atgcgccgtg
     2821 gccctgcgcg cgcgggggcg cgcggtccat ctggccgaga tgcgcgacca ggtccttccc
     2881 ggcgtgatcg acgcggacct cgcggcgccg gtcgcggagc acctgacgtt gcgcggcatc
     2941 gcgtttcacc tgggcgcggc cgtgggcgag gtcctcgggg aggacgcctt cgccggcgcg
     3001 cgaatcggcg attcgcggat caccgcatcg ctgtgcatcg ccgcgacggg gttcgcgccg
     3061 cacacggagc tcgcgcgcga aagcgggatc gagtgcgggc cgcgcggcat cgtcgtcgat
     3121 gactcgctgc gcaccgcgct cccggacgtg tatgccgcgg gggactgcat cgtcgcgtgg
     3181 agcgtcatcg acaaaaagcc ggtgggcgcg aagctcgcga caagcgccta taaacaggga
     3241 acggtcgccg gcgcgaacgc ggcgggcggc gcgatgacct atgccgggac cgcgggcgcc
     3301 ttcgtaacgg tgctgggcga tctcgagatc gcgggcgccg gctacacgac cgaggccgcg
     3361 cgcgccgcgg gttacgagcc cgtttccggc aagatctccg cgccggtcct tcccgactat
     3421 attccggacg gccacgccat caccgtaaag atactcgcgg accgtgcgac cgggcggctg
     3481 ctcggcgcgc aggcggtggg ccccgccggg gccgcggcgc ggatcaacgt cgcgagcgcc
     3541 gccatcgaat tcggaaaaac cctggacgac ctcgagcgcg tggagctcgc ctactgcccc
     3601 gccgtgagcg aggtgcacga tccgctcctg cgcgcggccg acttcgcgcg ccgccgcatg
     3661 cctaaacgat aatccccaag gaggaaccca tgagcgcaac gcccgacgaa ctgaaatatt
     3721 ccgccgacca cgtctggatc cgcatggagg acgacttcat cggcgtctgc ggcctcaccg
     3781 accatgcgca ggagcagctc accgacatcg tcttcgtgga gctgcccgag atcgacaccg
     3841 acgtccgcca gggcgagcgc ctggccgtgg tggagtcggt gaaggccacc accaacgtga
     3901 acgcgccggt gagcggccgc atcgtggagg tgaatacggc gctcgaggac tcgcccggcg
     3961 tcatcaatgc cgatccctac ggcgacggct ggatcttcaa gatcgacgtg aagggccgcg
     4021 tcgaatacga cgacctcatg gacgccgacc agtaccgcgc ccaggtcgag tagcgcccat
     4081 ggaccgcctg cgcgcgcaga tcgccttcat cgtcgagatc gacaagctca agcaggtgct
     4141 ccggcgcaac tacctggccg acggctcgcg ccgcgagaac gacgcggagc actcgtggtt
     4201 tttcgcgacc gccgcgctcg tcctgcgcga gcacgcgaag gaggcgatcg acatcgcgcg
     4261 cgtcgtttcc atggcgctca tccacgacat cgtggagatc gacgccggcg acaccttcat
     4321 ctacgacacc gccgcgcggg cggaccagca cgaacgcgag gagcgcgccg ccgcgcggct
     4381 cttcggcctc ctcccggacg accaggcgcg cgacctgtgc gccctctggg acgagttcga
     4441 ggccgggaaa acgcccgagg cacggtatgc gcgcgccatc gaccgcctct cggcggtgct
     4501 gctcaatcac gcttcgggcg gaaaggcctg gcgcgagcac ggcgtgcccg cgtcgcgcct
     4561 cagggaggtg aacggcgcta tcgccgacgg ggcgccggcg atttgggagc atgtccgcgg
     4621 catcatcgac gacgccgtgg cgaggggtat cgttgcggac gcggaccatt cctgactgga
     4681 tcgtccagcg cgtcccctcg ggcgagcgct atcgcgccgt gcgtcgggcg gttgacggcc
     4741 tgggcctatc caccgtgtgc gccgaggcgc gctgccccaa taccggcgat tgcctctgcc
     4801 ggggaagcgc cgccttcctt atccttgggc ggatctgcgc gcgcggctgc cgctactgcg
     4861 ccattgagac cggcgcgccc atcccgcccg atccatccga gccggaccgc gtcgcgcgcg
     4921 cagtcgcagc gctctcgctg cgccatgccg tgatcacctc cgttacgcgc gacgacctcc
     4981 ccgacggcgg cgcgggggcc ttcgccgaga cgatcacacg catccgcgaa cgcgcgccgg
     5041 gctgcgccgt ggaggtccta gtgcccgact tccgcctgag ccaggaggcg tccatggaca
     5101 gtattatcgc ggcgcagccg gacatcatca accacaatat cgaggtggcg cggccgcggt
     5161 atcatctgct tcgccccaac ggcgactacg acgcctcgct cgcgctcatc cgccgcgcgg
     5221 ccgcatcggg catccgcgtg aaatcgggac tcatggtcgg gttcggggaa acgttggacg
     5281 atatcgacgc gacgctcgcg gacttgcggg ctgccgggtg cggcatgctc acggtggggc
     5341 aatatctccg gtcgcgccgg gaatgcgttc cggtcgcgcg tttttacccg ccggaggaat
     5401 tcgaggagat cgcgcgccgc gcccgcggga tgggttttac ggccgtccac gcggcccccc
     5461 tggcgcgcag ttcgcatcat gccgaggtgt tcgcgcgatc cttccccggc tgaccattcg
     5521 ccggagaaaa cgaatacgaa gctccgggaa gcgctatctc ttcttcttct gctgcttctt
     5581 gatcttcgat gatgatgcgg acctggccgt cggctttttc ttcgcgggcg acgggccccg
     5641 ttttgtcgcg gtttttttct ttcccgacgg tccgcgcgcg ccggacgtct tcggcgcgga
     5701 tttctttttc cgcgcgcccg acgcagtcgc cccacgacgc gcgtccccag cgacggttga
     5761 tagcgtctgt ccccccagca catgtccggc gtccgaaccc gccgttgccg gggcgctttc
     5821 tctctctttt tctttcgaca gctcgtcccg ccgcagctcg aagccaatct tgatcgaatc
     5881 gggatagaag accaccttga gcggctcccg ctcgctcgaa tgacgcttga ggatgctgag
     5941 caccaaaccg atgcccatcc cggctccctc cgcgaaatca tcgctggcgg cctcgaagga
     6001 gtctcccgag agctccccat gccggatctt cctcagcagc cggcgcttct cgattgtcga
     6061 gatggaggcg ttgttcgcca cccagatggc gacggactct ggcgacgact gaaagaagat
     6121 ggtaatatac cggtcctccc ttcgggcgag ctcgacgagg cgccggttcg cgttctcctc
     6181 gatctcctcc ttaaacaagc gcatgaactc tcggtaatcc gaccccgccc cgacgccctg
     6241 tgggcgcgcg acatgccggt cgaagaggag cttgtaattc gccttggtgg cgttcaccgc
     6301 gagctccttg aggcaggtga acagctcata ggagtaaccc agcaggtcct gccgcgccag
     6361 caggagatca attatttttc gcacgagctc cacggcatcg cggtccatgt cgtaacagcg
     6421 gatggtgatg cgccactcga ggtcccggat gctgagcttg ttcgcggcgg cggcgatatc
     6481 ggagacgatc ttctccagtc gcggttgagc ccgcaggtct atcacgcgga gttttttgag
     6541 caggttgatc tcccgctcgt aaaagggatc gcggcagaaa tacttgaggt cgggatcgta
     6601 catccacttg ttccgcgcca gaagcttgat aaaccggtcg cgctcccaga tgcgcagcgg
     6661 ggcattgtag aaacgggcgt tctcgttctc cagcagaatg ccgaacaggc ggacgatgtc
     6721 gtcctcgtcc gcgtaatagc gcacgagcgt ctcggccgcg gtttgcgccg gcagcggaat
     6781 gctgggagag aagcccgact cgcgctgcac gctaaagccc ggcgtgagcc gctcgcccag
     6841 gtagttgatc tggcccgtat tcagtgagtt cgcgaggatc ttcaggaact cttttcgtat
     6901 ctcgatgctc atgggccagc tccacgtcgc ggcataccag tcattcgccc gccctcccgc
     6961 tacgccatga tgtcgtcaat gtcgcccgcc tccatccgct gaagccgctc gcgcagcgca
     7021 tccagcgacc cggtcatctc cgcgatggag cgctgccgca cggtctgcga gaaatcgcgt
     7081 ccggcccatt cgaagcgcgc gctgaacgtg aagcagaagt ccttcgcctc gcgcagggcg
     7141 tcgcgcacgg ttttcatcga gaccgtgtag cggttcggcg cgggcgactg cgcggccagg
     7201 ataacgcgat ccgcgctcgg caccgcgcgc accccgatct cggcggcccc gagcggcccg
     7261 gcggccgact ccggaagcga ggcgtcgcga aggcaatcga acagaaggag ccccatcgaa
     7321 tagaacgcat cagtgaccgt cgaaagcgcg ttcataaaga tggcctcctc ccggctcagc
     7381 gtctccttct tgagcaaggg ccacccgtat cccttgcgga cgcactcgag aaatacccgg
     7441 ctctcgcgct tgatggcctc gatctgccgc tcgaacacgt ccggcttgaa caatgagatg
     7501 aagcgcttgt cctgctccac gcgcacgtac accccctccg cgatgagcgg ctcgtaggcg
     7561 aaggcgaaca cctcgcaggc gtccgccgcg gaaccggcca agttgttctt cttgattcgg
     7621 aaggtctcgt cattattatg atagagataa tacagaagat actcgacgat gtccagggcc
     7681 ccgtgcttca cgggataccg ccgcgaaagc gccgcgccga tgtccgtgac cagctcgatg
     7741 tcgcgcgtga gcgccgcgat cgcctcgcga agccgctcct ggaacttgcg cttgcgcgcg
     7801 atcatcgcct cgtagaggtg cgcatcggcg cgatactcgg cgtcgtcgac ctcctcgagg
     7861 cggaccatcg cgcggatgtc ctcgccggtc agcgtccggt gataggcgca ctcgaacagc
     7921 gggaccacga actcgcggtc ggtctcgccc gagaggagct ggcgcaccgc ggccacggcc
     7981 cgctccgtct ggcgggaatc gccgccgtcg gagctcacga tgcgtcgctg cgacttgcgc
     8041 agcgcttcga ccagccggtc gccatacacg ccgtttctcg tgagcgagaa atagtacccg
     8101 agaaagggat tgatcttctt gacggcgctg cgcggttttc tgatgtccag caggacgtta
     8161 aagatgctcg cgtcggccgt gaggcgctcc atctcgccga tgagattgaa ctcgagcggc
     8221 gagagtacgt cggccccggc gcggctgagc cagccctgcg cgtacatccg ccggaagggc
     8281 tcgcgcagct cctccatgat cacgacaagc ttttgcacga agcgatacac ctgcatggag
     8341 ggccagaaga ggtcgccgcc ggagaaaaag ctccagaagc tccaggatat tgttccggcc
     8401 gccttgtatt tttgccggaa ctcgcgccgt atcccaaagt acgaaaagag cgacacgaag
     8461 gcgctcgagg ggctttttac aagaatggaa cggtcgtagg cgcgccgctg gtactgcggg
     8521 aggatgtgca ggccgccgat ggcgccctgc tggcgcttga gcttctcctc gtcgtccacc
     8581 agatcgtcca tgaggccgcg cagcttcgcc cgcatctcga cgatctcgcc ggagtcggcg
     8641 ctgaacttgc gcaggcgctc cgcctgctcc cgctcgcccc gcgtggcagg acgggcgatc
     8701 acttccttga tgtactttcg ctcatccatc ccgcatcagc tctcgcatcg actcggtcgg
     8761 tccgcccccc atcatgggat tcgattatag gataccgccc gcggttcgtt ctgtccagaa
     8821 catatttttt acttgcgcgg gaaatcgcgc tagagtattg cccatactac ctattccgat
     8881 ccggcaatcg acacatggac cagcaccccc atcaacccac cgaacaaaag actgacgatt
     8941 gccgggaccg cctcggcttc ctccaagggc tcattgacgc catccccaat cccatcttct
     9001 acaaggaccg tgacggacgc tacctgggct gcaacaaggc cttcgaggcc tatctcggga
     9061 ccacgcgaac cgagatcgtc ggaaaaacgg tgttcgatct gtccccgcca gacctcgcgg
     9121 cgcgctatca cgagatggac gagcggctct ttcgcgacgg cggcgtgcag gaatacgagg
     9181 cgtcggtgct ccatgacgac ggcaaccggc acgaggtgga gttccacaag gcggtgttcc
     9241 tcgcgccgga cgggcggccc caggggcttg tcggggtgat cctggacatc accgagcgca
     9301 aacgcgccga ggaggcgttg cgcgtctccg aggagcggtt ccggatcatc tacgagcagt
     9361 cccccatcgg catcgagctg tacgacgagc gcggcgagct cctcgagatc aacgccgcct
     9421 gcctggagat ctttggcgtg gccgatcccg acgcggtgcg cggcttccgc ctcttcgagg
     9481 accccaacgt aagcgcgagc gcgaagctgc gcctgcaggg cggcgaggag gtccgctacc
     9541 gcacgccctt ctccttcgac ctggtccgcg agcacgggct gtataaaacc tccatgtccg
     9601 gcatcatcca tatcgacgtc atcatcaccc ccatttcggc ggccgatgga gcgccgcccg
     9661 ctcggtacct cgtccaggtg caggacgtca ccgaggccgt ccttcgcgag gagaacctga
     9721 actacctcag catgcacgac cagctcaccg gcctgtacaa ccgcgcctat ttcgagatgg
     9781 agatggagcg cgtgcagaag agccgcatgg atacggcggg cgtcatcgtg tgcgacatcg
     9841 acggcctcaa ggcgatcaac gacacccggg gacaccaggc cggcgacgag ctcatccgcg
     9901 ccgcggcggg catcatcaga aaatcgttcc gcgacagcga cgtggtggcg cgcatcgggg
     9961 gcgacgagtt cgtcgcgatc gtcccgaact gcaccccgga gatactcgtg accgcctcgc
    10021 tgcgcatcga ccggaacatc gcggcgcaca atctcgccgg gagcgggccc gcggtggccg
    10081 tctccatcgg cgccgcctgc ggccgcctgg ccgaccgccg cgtcatcgac atcttcaacg
    10141 acgccgacgc gaacatgtac gccgacaagg cgaaacgcag gtccgggcgc ggcgcatcgt
    10201 gaccgacggt cgcctcgagg cctcctgtaa accgacgggc gggaagctcg tccgtgtgtc
    10261 cctttcgctt gccggcgggc ggatcgagcg catcgccatc acgggcgatt ttttcatcat
    10321 gccggaatcg ggcgtcgagg atatcgaggc cgccctcgtc ggcgccgacc ccggtgcggt
    10381 ccgtgcgcgt atcgatgccg ttgtgcgcaa tcgcggcctt cgcctggccg gcgtctcccc
    10441 cgcatcgatc gccgaggccg tgcggcgcgc cctcgaaggc ggggagcgcc cggcatgagc
    10501 gacacgccgt ggcgcggcat catcaccccg cccgcaccgg gcccgtacaa catggggctc
    10561 gattacgccc tcctcgagtc cgtcgccggc ggttccgcgc cggtcgtccg ctgctaccgc
    10621 tgggcgccat gcgcgatcac catcggctat ttccaggacc cggaacgcga ggtggacctc
    10681 gcagcggcgg cccgcgacgg cgtggcggtg atccggcgcg tcaccggcgg cggcgccgtg
    10741 ttccacgact gcgagctcac ctacagcatc gccctccccg agcgcgcctc gccggaactg
    10801 cggtccatcg cgggctccta cgacatgatc tgcggcgccg tcgcgcgcgc gatctcccgc
    10861 ctcgggatcg acgcctcctt cgcgccggtg aacgacatca tcgtgggcgg gaggaagatc
    10921 tccgggagcg cgcagacgcg ctgcggcggt gcgatattgc aacacggcac cgtgctcatc
    10981 gacgccgacc gcgcgcggat gtccgcctat ctgcgcgcgg acccggagaa gtcgcgggag
    11041 cgcgacgcgg ctggaccggc cgagcgggtc gtcccgctgc gcgacctcat cggcgatcgg
    11101 gcgctcgcgc cggaaacata ctcgacgctc tgcgacgcga tcatcggctc attcgccgga
    11161 acattcggcc gcgcgattga accgtcgtca ccgacgggcc ccgagagcca acgcgccgcc
    11221 gcgctcgccc gggatctctt tggcgacgac gattggaacg ctcgccggat ccgcccgccg
    11281 ctgtaaccac gggcatgccc cgcccgcgcc ggacggcgtc ccgccggaat atttccttga
    11341 catctgacgc aggcgttttt ttgtgatata gtgcgtatcg ggcaaagcgc atttacggaa
    11401 ggatcgtgcc gcgttccgtc gatgaagcgg tgcgacgctc atcatacaac aaaggagaga
    11461 ccgaccatgt cacatcatga taaagaccat gttcccatgc cgctgaatac gccggccttt
    11521 tcgtgcacgg gctgcggcgc cgttgcgctc aacgcggatt cgctgtgcca gcccgcgggg
    11581 cgggtgcgca aatccgactg gtgcgggacc gaaagcatcg acatgccgcc gttttgcctc
    11641 aacgaccggc acacgctgcg ctaccggtgc aagaagtgcg ggcggatatc catcgacgcg
    11701 ccgttgttgt gcgagccgga gaaggtcccc gtcaaggagt gacggagtcc ctacgcgagc
    11761 cggtataccc gtatcggctc gcgcaggccc ttgacctcca ccggccccag gtcctccccg
    11821 acgaacccct cgcccgcaac ggcctcccgc acggcggacg acaccagcat ctgtgacccg
    11881 aactgtttat tgagctgctc gatgcgcgag gcgaggttca ccacgtcgcc gatgatcgtg
    11941 tactccttgc ggaccgacga cccgacgttc cccgtgacgg cgcgcccggc gtggagcccg
    12001 atccccaccg tcgtcgccgg gacccgcccg gcccggacct cctcggccag acgttcgatg
    12061 atggcgcgcc ccgcgcgcac ggcgttcagg ctgtcccgcc cgtccgagag cggcgcgccg
    12121 aagaccgcca tgaagccgtc ccccaggaac ttgttgatga tgccgttatg cgcattcacg
    12181 atgtcgatca tgaaatcgaa gagcgtgttg agatacccga tgacatattc gggatcgcgc
    12241 ccggcggaga aggcggtgaa gttccggatg tcgagaaaca tcatgcacac atgccgcatc
    12301 tcgctcgaaa actcgccctt ctgctcgagc agcttgttca ccaccgccgg ggagacgtgc
    12361 tggccgaagg tgtccatgac gcgttcgcgc tcctggacgg cctggtgcgc gcggaagatg
    12421 cgccgccgga cctgcagggc cacgaagccg gtcacgatgc ccgcgaccag gaggagcgcc
    12481 gcgcgtccga tgtgctgcgc ctgcgagagc aaaagcgggt tcaccgcctc ggcatgcggc
    12541 aggccgacga gccagagggc gagcgccagg tattcgacgg cggccagggc cccggtgaac
    12601 aggcacagcg cgatgctcag ccggaaggac gacagggcga tgaagatgaa gtagaggaac
    12661 acgatgggcg cattgagcgc cagcgacggg gcctccgcgt acaggatcga accgatgaag
    12721 acgatgacgg tggggccgct cacctcgacg atggcgtttc cgtaccgcat caggcgcggg
    12781 aacggccggt ccgtccgcat gaagtacccg aacatccagc gcacgaagat ctcgtagacc
    12841 cccgaaacgg cgaagaagat gatgtaccac gcctgcacgg gcagcccata gcggacatgg
    12901 aacatctcgc gcggaaagcc ctgcgggaag agcagcgttg ccgcgccgat gaacagcccg
    12961 ccgagcagaa agaacgccgc gatgatggtc gcgcgcaggc gctcgctggc gagcatctcg
    13021 ccggtaaagg tatcctcgaa cgacccggcc cgggagcggt cccgggacac gaacacgata
    13081 tccttcaacg tggccacggt gccatcctca cgcacgccgc ttcatgacga agagcgtcac
    13141 atcgtccttc atggtgtatt cgttcgcggc gtcgaggatt ccccgcttga tcccctcggg
    13201 atcgcgaccg ccgagcgatt cgaacacctg tttcaatctc tcgtttgaga acagttcccc
    13261 gtgggggctc atcgcctcgg tgatgccgtc cgtgtaaagc aggagcgtat cgccgacatg
    13321 cagcgtgaac tcgaaatcgc ggttgagtcg ccccaggtcc tccatgaagc ccagccacat
    13381 cccgtttgtt tcgatcacgt ccaccgtccc ggtggccgcc cggaacacgt agatgtcctg
    13441 gtgcaggccc gagaagagga ccctgccgcc cttcagaaag gcgaaggccg tgatcgtcat
    13501 gtacttgtcc tcgtcgagct tcttgatgtt gtaggtgatg gcgcgattga tcacccgcaa
    13561 cagcaccgag ggcttgacga ggggcttccc gcgcaggatc acctggatcg aggtctgcac
    13621 catcatcatc accagccccg cggggacgcc gtgcccggac acgtccccga tgatcaccca
    13681 gtcgacctcg tcgtcgttga tcacgtcgta gtaatcgccc cccacctcgc tggcgggcat
    13741 catgtgggcc gcgatctcgt agccggggat ggacggatgc tcgggaagga ggatcgtctg
    13801 gatgcgcttg gcgagctcca tctcgcccca gagcgcgtcg cgcgccacct tgagctcggc
    13861 ggtgcgccgg tccacgatct tctcgaggtt ctcggcgtgg ttgtccagcc gcttcttggc
    13921 cgaacgaatg gtgcgcacca tccggttgaa attatacgtg agaaagccga tctcgtcctc
    13981 caccttgatg gggatgcgga tgtcgaggtt cccctggcgc acctcctgca gtccgtgcag
    14041 gagcgtctcg agcggattga tgagcgcccc caggaagaag aagcggaacc cgaccatcac
    14101 caccatgagc gtggccacga gcatgatcac catcttgagc gccgcggggt gcagatacgc
    14161 gcggtagctc tcgtaggtaa agcccacttc atggatgatc cgcccggcgc cgtcgccgcg
    14221 catatacgag acgaagtggg cgccgcggcc cccgcggtag atccgtactc ccgccggctg
    14281 catcggcgcg atgtagtcca gcacctcgcc cttgagcgcg aacccctcgg cgcgcgagcg
    14341 cttcaggtgg tcgcgcatcg ccgtcgcgaa cgggcggaag gcgtcgtcgg tcttgttcag
    14401 atagacggcc aggctcgcgc ggaaatcgtc gctgggaaga ttgcggatct tgttcgcgcg
    14461 ataacgaagc gccttggcga ggtcctcgac gcgcgcgacc atgagccgcg ccgggtcggg
    14521 cgttgcgcgg ggaatcgatg ccgcgattcg ctccaaggcc gcgcggtacc cgtcgaagcg
    14581 attgtgcccg gcggcgagca ccgccggcag ttctttctcg aacgagtcgg cgggaagcgc
    14641 cgcgatcttc tcgacgtacg gcgagcgctg gccgtgcagg tccggcagg
//
LOCUS       DAORHF010000076        14683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3167_length_14683_cov_5.821028, whole genome shotgun sequence.
ACCESSION   DAORHF010000076 DAORHF010000000
VERSION     DAORHF010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14683
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3167_length_14683_cov_5.821028"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1635
                     /locus_tag="PKJ16_08175"
     CDS             262..1635
                     /locus_tag="PKJ16_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNV47002.1"
                     /translation="MHKLENMKLDAIIVGTGPGGATVAKELAKRGKQVLMLEWGPGGP
                     VKGNIIQYFFQQCFPGKGMLITNNFLGMVRGITTGGSSLFYYGTCFPVPHDMLKKYGI
                     DVRREEEEARRELPIATLKDEMVTPMAQRIMESAQALGYAWTPLQKFMYQDRWRPGMK
                     FGYYGDKHRVKWSARMHAEEAVAMGAVLLNKARVSNIILDGNRATGVEFKMKGRSYKA
                     FAPKVIVAAGGIGSPVILRKMGLKNVGYNFFYDPLITVCGKVDDVRKRIDEIPMTAGV
                     HFPEDGIVMTDMAIPTLIDKAFTAQVLRFWRLFETRKTLRIMIKVRDDLAGRLTDSGG
                     VRKKLTQADRDKLMKGYDIAKRILEKAGAKGIYKTWYLAAHPGGTVKMGEFLDANLKV
                     KNYENLFVCDCSVIPEPWGLPPALTLVCLGKRLAKHLAGEKTGANAAAPSKAKPAKNK
                     RKKAGRR"
     gene            1666..2316
                     /locus_tag="PKJ16_08180"
     CDS             1666..2316
                     /locus_tag="PKJ16_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47003.1"
                     /translation="MPKLVDHDEYRRELLEKCFSIFTRKGFSGVTMREIAKEIGVSTG
                     TLYHYFPTKESIMEHMFSYVQETNVNAFTTITETLDSVPEKLDLFVEKWKEFGAFYQS
                     MMLLAIDVLRNMSSEDSEKIFTNFSNHYTGAMARELHVSEDEARALFIYLLGIILHSL
                     LTPRFMPYERQIDILKGMLEKMLPGDAGFSGAGPGGLVGSFLDKCTTAAPDNKEPR"
     gene            2339..3802
                     /locus_tag="PKJ16_08185"
     CDS             2339..3802
                     /locus_tag="PKJ16_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47004.1"
                     /translation="MNDSLHTPAPQDTPELTASLARALVRTSGFRAIIRALVPEVLDA
                     WAGSSRSRRALARLIGRDILKSFDAAGDDAGRDAMADIMQRPDIARRLGELLPEALNA
                     AIDTAAAAIDGIASLPPREASGVARAVAETVDAKRAGEIVTGLVRCVARVHEDDPTFF
                     SKALQPCFRSWIEGIDFGEVKETVDRSSDDIAATVAMANRELWRYPAKVICLLSLIPS
                     IVAILARSATETVKPLNELAPDLLTDVVLSFVREIDGAHVGALINEISELVRKIHTGS
                     ALIGDAGNPLLPQTVAALAGSTLDAVDVHKLVRARSLIRDLGETTFDAVLEKIEQRPE
                     LARELFFGHFTALAAFARRWAAKTEAVERLFDDDALAAEFARGLHELDMQELAATAGR
                     FCALLNRIHGSSPGVVRDLLSQFFGSLDGYEARETAEWLMADIVESIKPIAPEVLPPV
                     LNGIADLITPDDGRSDELARSVARLRDAILGAEVKSK"
     gene            3811..4947
                     /locus_tag="PKJ16_08190"
     CDS             3811..4947
                     /locus_tag="PKJ16_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47005.1"
                     /translation="MEAATVLNNRRVGEWKAQGGRVVGYTCSYVPDELFHAAGILPYR
                     IRGIGASETTIGDTYFGPFICSLPKCMLQLAGEGKFDFLDGAIITPGCDSMRRLDECW
                     RKAGDDNPGVLPKFFFHFGVPHKYSDYTIAWFTVEIQRLKAAVEGHFGVVITDEKLAA
                     SIRLFNRGRDLLEAFDAFRTAEDPPIGGADALAVIMAGTTMPRERYTELLEGLVGGLG
                     RSASRITGKARLMLVGSANDDVELVCAIEGASAIVVADNLCFGSRFHADRVSEDGDPI
                     EALARRYLAKNECPRVYGEYKARLATLRHKAEQARVDGVVLQNIRFCDLHGSENGLFE
                     RDLEAAGIPCIRLEREYGPLVETGRMKMRIDAFIERIRQRRKTA"
     gene            4944..6350
                     /locus_tag="PKJ16_08195"
     CDS             4944..6350
                     /locus_tag="PKJ16_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47006.1"
                     /translation="MRPETREYQFDWMLWSCFSAASKLNAGTPKEYRRMLEYIPSFRG
                     VVETVLDAGEPGQRFIEICATYLDNVINAKSRGQKTAITTFCFSPAIFYAMDIMPICL
                     EVISVMMTFTYKRGTTEFLDYCNEAGYTETSCSSQRGSIGAYLAGLGAEIDMIVTDTP
                     GVCDTNANAFAFAAAYLDKPFFQLDMPPDLTGERSNEYHRADYTALISFLEQHSGKRL
                     DLERLREKLIEVQKQDEMIAELEELARMVPNPLPVVYNFMVYASRFLFAGMPECTRTL
                     ESLLALARQNAAEGKSGISGGAERLRSYFCYIDHYVQNLKLWQMLDANGICYQGNILS
                     RSWASNAPHVREFGTQEAAYTLDTANLDGLIDTMAMLNSRMPMIKSIRGPYDAPNMWL
                     QDTLSLATMYNVDFIVYNGTPGCRNTWGMVKLLARDTEKAGFPTFIMYADAFDERVQS
                     WETTRDRFMEFLNVRRLL"
     gene            6347..7141
                     /locus_tag="PKJ16_08200"
     CDS             6347..7141
                     /locus_tag="PKJ16_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV47007.1"
                     /translation="MTCVAGCDVGSLTSKAVIMDGDRIVQSVIMKSKAKPWESADEVM
                     RQALEKAGMSMGDLAYCVGTGYGRDKMTFINEAVSEIACHGKGAQWLVPTARTVIDIG
                     GQDCKAVKLDDAGNMVKFITNDKCASGTGRFLEVMAKVLGVELADLGALSAKSKSPVT
                     LASACTVWAQADVIQHLNDTVPLEDIAAGVNAAMASRMAVLANNIGVARDVCMTGGVA
                     KNEGVVASLEKALGVRIKRIRKSDPQLAGAIGAALFAKEKVDGGMR"
     gene            7138..7914
                     /locus_tag="PKJ16_08205"
     CDS             7138..7914
                     /locus_tag="PKJ16_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV47008.1"
                     /translation="MIAAGCDVGSLTAKAVILKDGAIAAREVMRAAASPERSAREVME
                     RAVAHAGVSMADIARCVGTGYGRKHIDFVQSMESEIACHARGAVWQVPSARTVVDIGG
                     QDAKAIRIDETGNVERYAYNDKCASGTGRFLEIIADALEIPLDEMGAVSQKSTEQLTL
                     SNQCVVFAETEIISLVSEGREIADIVSALHRAVANRAASLARGIGVAGDAVMTGGVAK
                     NAGMFLALERALGVPLKSVPDPQINGALGAALFAQDALAK"
     gene            7911..8657
                     /locus_tag="PKJ16_08210"
     CDS             7911..8657
                     /locus_tag="PKJ16_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47009.1"
                     /translation="MNLFDDSVGRLRTFLERKQSAGELRTTQLPATTGWPAGERGNIV
                     LAADTGVELGNPRVESASFMVWTEAADAVRDGAVHVVGPDLAESAGRSLPFGKAVILR
                     VDGMNEENCYERHRALELIRYRLNLKGYMMRAVSQYLREWSRVSVEAIDGGFSFATLA
                     GTLHSMYRAVEYVSAVEFVFVTSSPEDVRALGETGARVERLIGAMNKMAQEMSFDCGV
                     CDYTDVCSEVEDLRSMRKNAAEGGAHATRH"
     gene            8641..9459
                     /locus_tag="PKJ16_08215"
     CDS             8641..9459
                     /locus_tag="PKJ16_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47010.1"
                     /translation="MQRGTEGRTQAIIALCGKGGVGKTALSASIIRALVRDPEKRVLA
                     IDADPAVGLATALAIEVRRTVDEVRTDFIELVKQRAAGDKERVLSLLDYEMLGALHEA
                     GALAFMAIGRPETEGCYCQVNDMLKDIIASIAGNFDYVVIDAEAGVEQVNRRVLERVT
                     HLVLVSDLSAKGLRVAETIHDVAHNAIGFERAGLIVNRLRDGAELDAVPLPASLPFLG
                     AVPEDECIRRADIEARSFLDLPDAPAFAALAECLDKLGIDAGGRAACTAGRHSA"
     gene            9456..10580
                     /locus_tag="PKJ16_08220"
     CDS             9456..10580
                     /locus_tag="PKJ16_08220"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47011.1"
                     /translation="MTAPMTGLAGLCADVRNELIEEAMGRGMIPLGYTCSMVPRALLA
                     VGTLFPVRMRAPGVAGTECADVYLSSVICSYSRSLLEFALDGRYDFLGGWVFVACCDH
                     LRRLCDNLEYNAKPDFSHTLDIPHRGGPAALEWTVEELRRLARAIERHFDVDMGDDAV
                     RAAMVAEGELVDALRELGALRKKPQPPFSGTDYHRVLMAAQSAPPARALELVREFREA
                     MGDASDTAPYRARFLLVGGSLDDPAYTAVIESQGGLVVADRYCTGSYPWLDPVDAGGD
                     PFVALARQVLDGNRCPRMMEGFAQRVNYIVDLAREYAADGVIIQSIKFCDTWGVEASA
                     LVTALREAGVPVLRLEREYRQSGEGQLRTRVQAFIESMGK"
     gene            10582..11985
                     /locus_tag="PKJ16_08225"
     CDS             10582..11985
                     /locus_tag="PKJ16_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47012.1"
                     /translation="MGTPGIADYAVTSKYKLLNLAVIALMGLRNGPYDMVRDLRRYPW
                     LWHLLKVNRLIDKLTAGRTGNYREAISIIVHGVTKEVVDLLDGIYFRQDRLVLHEDMV
                     PPEILRAMGLSPWMPEVLGILLPMLEPSAMEEYIDVAENEGVPADVCSLPKAVIGTAL
                     RDMLPPARAVVTSNLPCDGGMTSYLYVEKMTAAPTFRLDIPFNFKDERADAYFANELR
                     RMIAWLEEHTPGRIDWDRLKEICEERNRMVEQELELWDMIALRPAPLAAEAVFLGHLW
                     FMNVCSGVPAATEVFRRLVELAKENLARGVAACPDERYRTLLWNPPTMHFIDLFTWAE
                     RAYGVTCIMDSMSFNRLPFIDTSSEESMLRGIGRNIMNGPMARHTRGPMANYFDDMFH
                     IIKRFDIDMLWMAGHIGCKNTQALNAIMREKCRDVGVPVLIIDYDLSDTRIVPREGIM
                     TQVDSFMENVMHAKRRA"
     gene            12146..12940
                     /locus_tag="PKJ16_08230"
     CDS             12146..12940
                     /locus_tag="PKJ16_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV47013.1"
                     /translation="MKKKFFAGCDIGSTTGKAVIIDENGVVASSIVPREVDPEETAHL
                     ALAEAFKAIPDVNTHSDLAYLVGTGYGRSEIPFANENVSEISCHAMGAYYCDPAIKTI
                     VDIGGQDVKGIAVNGDGSVKDFAMNDKCAAGTGRFFEAMARIFRMELSEFSDLSLASK
                     RAIPVTAQCSVFAETEVISLLAQKNPPADIAAGVQGAVAKRCFTLLKRVGVHPTVTIT
                     GGCAKNRGLVRELERVLRVEMASLSVDPQIIGALGAAVYAKKKARG"
     gene            13522..14361
                     /locus_tag="PKJ16_08235"
     CDS             13522..14361
                     /locus_tag="PKJ16_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47014.1"
                     /translation="MKKHSIVALIGCIIFLVGCKTSYEYIITGKQYPPLKKDDPVKMV
                     FLEDKGRYEELGIVEVTGKTLMKRVDAAVDVARKQGGDVLMIKGVRDEKAMEELRSQE
                     DPSRPLKQSFLVLRTRPDDPNERLDDEDAPAVRSGGPKKSVKAPSIRTGVDDEGALDE
                     ADPSGLKRATWRLLVEEFEMLRGERFRASMYPLASSRKIPPSIDYHADGAKRLITMAT
                     RGRRHFAYLIVPKKMEQRIMRAKRFKRSFEFVYTPVDVYRRGGKTYPVLEFVREIEGA
                     GSK"
BASE COUNT         2805 a         4426 c         4841 g         2611 t
ORIGIN      
        1 cagagcccca agcgacaggg ttcagagccc caagcgaagt tttccagcca acacgctttc
       61 gtcaatttga ttttcatcat acagtataaa tatagactca ttgaatataa taagtatata
      121 ttttgtgcac actatcttaa aaatataaaa aaatctaaaa aatatacatt tcactattga
      181 cgaaagtcta gccggtatgt ttatttataa caactgttcg atataaataa atccaattgt
      241 catactattc tggaggaaca aatgcataaa ctcgaaaaca tgaaactcga tgccattatc
      301 gtcggaacag gtccgggtgg cgccacggtt gcgaaggagc ttgcgaaacg cggcaagcag
      361 gtgctcatgc tcgagtgggg gcccggcggt ccggtcaagg ggaatatcat acaatacttt
      421 ttccaacaat gctttcccgg aaagggaatg cttattacca acaacttttt ggggatggtg
      481 cgcgggataa cgaccggcgg gagctcgctg ttctactacg ggacatgttt tccggtgccg
      541 cacgatatgc tcaagaaata cgggatcgat gtgcgtcgcg aagaggagga ggcgcgccgg
      601 gagcttccca tcgccacgct caaggacgag atggtcacgc cgatggcgca gcgtatcatg
      661 gagagcgccc aggcgcttgg gtatgcgtgg accccgctcc agaagttcat gtatcaggat
      721 cgctggaggc cgggcatgaa attcggctac tacggcgaca aacaccgggt gaagtggagc
      781 gcgcgcatgc atgcggagga ggcggtcgcg atgggcgcgg tgctcctgaa caaggcgcgc
      841 gtgtcgaaca tcatcctcga cggcaaccgg gcgacgggcg tcgaatttaa aatgaagggt
      901 agatcgtaca aggccttcgc gccgaaggtc atcgtcgccg cgggcggcat cgggtcgccg
      961 gtgatcctcc gaaaaatggg cctgaagaat gtcgggtata atttcttcta cgatccgctc
     1021 atcaccgtct gcgggaaggt cgatgatgtt cgcaagcgca tcgacgagat cccgatgacc
     1081 gcgggcgtgc acttccccga ggacggtatc gtcatgacgg acatggcgat ccccaccctt
     1141 atcgacaagg ccttcacggc gcaggtgctt cgcttctggc ggctcttcga gacgcgcaaa
     1201 acgctgcgca tcatgatcaa ggtgcgcgac gatctcgccg gacgcctcac cgattccggc
     1261 ggcgtgcgca agaagctcac gcaggccgac cgcgacaagc tcatgaaggg atacgacatc
     1321 gcgaagcgca tcctcgagaa ggccggggcg aagggcatct ataaaacctg gtacctcgcc
     1381 gcgcacccgg gcggcaccgt gaagatggga gagttcctcg acgccaacct caaggtgaag
     1441 aactacgaga acctgttcgt atgcgattgc tcggtgatcc ccgaaccgtg gggcctgccg
     1501 cccgcgctca cgctggtgtg cctgggcaag cgccttgcga agcatctcgc gggcgagaag
     1561 accggcgcga acgccgctgc gccgtcgaag gcgaagcctg cgaagaacaa gaggaaaaag
     1621 gcggggcggc gataaccgcg cctcaccgac aggagatgac gcatcatgcc taagctggtc
     1681 gatcacgacg aataccgaag ggagctcctc gagaagtgct tctccatctt cacgcggaag
     1741 gggttttcgg gcgtcaccat gcgcgagatc gccaaggaga tcggcgtatc caccggcacg
     1801 ctctatcatt acttccccac gaaggagagc atcatggagc acatgttctc ctacgtgcag
     1861 gaaacgaacg tgaacgcctt caccacgatc acggaaacgc tcgattcggt tccggagaag
     1921 ctcgatctgt tcgtggagaa atggaaggag ttcggcgcct tctatcagag catgatgctg
     1981 ctcgcgatcg acgttctccg gaacatgtcg tccgaggact cggaaaagat attcacgaat
     2041 ttttcgaacc actacaccgg cgccatggcc cgcgaactgc acgtctcgga ggacgaggcg
     2101 cgtgcgctct tcatttatct tttggggatc atcctgcaca gcctgctcac cccgcggttc
     2161 atgccctacg agcggcagat cgacatcctc aagggcatgc tggaaaagat gcttccgggg
     2221 gatgccggat tttccggcgc gggacccggc ggcttggtcg gaagctttct cgataaatgc
     2281 acgacggccg ctcccgataa caaggaacca cgctgaatgc gaacaggagg aacgcgcgat
     2341 gaatgacagc cttcacacgc ccgcgccgca ggacaccccg gaactcacgg catcgctcgc
     2401 ccgcgcgctg gtgcgcacga gcggattccg cgcgatcatc cgcgcgctgg tgccggaggt
     2461 gctggacgcc tgggccggtt cgagccgaag ccgcagggcg cttgcccggc tgatcggccg
     2521 cgacatcctg aagagcttcg acgcggctgg cgacgatgcg ggccgcgacg cgatggccga
     2581 tatcatgcaa cgtcccgata tcgcccggcg actcggggag cttctgccgg aggcgctcaa
     2641 cgccgcgatc gacaccgcgg ccgctgcgat cgacgggatc gcgtcgcttc ccccacggga
     2701 ggcctccggc gtcgcgcgcg ccgtcgcgga gacggtcgac gcgaagcggg ccggggagat
     2761 cgtaacgggc cttgtgcggt gcgtcgcgcg cgttcacgag gatgatccga cctttttctc
     2821 gaaggcgctc cagccgtgct tccggtcgtg gatcgagggg atcgattttg gcgaggtgaa
     2881 ggagaccgtc gatcggtcgt cggacgatat cgccgcgacg gtcgcgatgg cgaaccgcga
     2941 gctctggcga tacccggcga aggtcatctg cctgctttcg ctcattccct cgatcgtcgc
     3001 cattcttgcg cggtccgcga ccgagacggt gaagccgctc aatgagctcg cgcccgacct
     3061 tttgaccgac gtggtgctct cgttcgttcg cgagatcgac ggcgcgcacg tgggcgcact
     3121 gataaacgag atctcggaac tggtgcgcaa gatccatacg ggtagcgcac tcatcggcga
     3181 tgccgggaac ccgctcctgc cgcagacggt cgccgcgctc gccggttcca cgctcgacgc
     3241 ggtcgacgtg cacaagctcg tgcgggcccg gtcgctcatt cgcgacctgg gcgagacgac
     3301 gttcgacgcc gtcctcgaga agatcgaaca gcgccccgag cttgcgcgcg agctcttctt
     3361 cggtcatttc acggcgcttg cggcgttcgc acgccggtgg gcagcgaaaa ccgaggccgt
     3421 cgagcggctc ttcgacgacg atgcgctcgc cgccgagttc gcccgcgggt tgcatgagct
     3481 cgacatgcag gagctggcgg cgaccgccgg gcgcttctgc gcgctgctca accgcatcca
     3541 cgggagttcg ccgggcgttg tgcgcgatct gctgtcgcag tttttcggtt cgctcgacgg
     3601 ctacgaggcg cgcgagacgg ccgaatggct catggccgat atcgtggaat ccataaagcc
     3661 gattgcgccc gaggtgctgc cgccggtgct caacggcatc gcggacctca tcacccccga
     3721 cgacgggcgg tcggatgaac tcgcacgatc cgtcgcgcgc ctgcgcgacg ccattctggg
     3781 ggcggaggtg aaatcgaaat gaaggaattc atggaagccg cgaccgtcct gaacaatcgg
     3841 cgtgtcgggg agtggaaggc gcagggcggc cgcgtggtcg gatacacctg ttcctacgtg
     3901 cccgacgagc tctttcacgc ggcggggatt ctgccgtacc gcattcgcgg gatcggcgct
     3961 tcggagacga ccatcgggga cacctacttc ggtccgttca tctgcagcct gcccaagtgc
     4021 atgctccagc tcgccggcga gggcaagttc gatttcctcg acggcgcgat catcaccccg
     4081 ggctgcgatt cgatgcggcg tctcgacgag tgctggcgca aggccggcga cgacaatccc
     4141 ggcgtgctcc cgaagttctt tttccacttc ggcgttccgc acaagtattc cgattacacc
     4201 atcgcctggt ttacggtcga gatacaacgg ctgaaggcag cggtcgaggg gcacttcggc
     4261 gtcgtgatca ccgacgagaa gctcgccgcc tcgatccggc tgttcaatcg aggacgcgat
     4321 ctcttggagg cgtttgacgc cttccggacg gcggaggatc cgcccatcgg cggggccgat
     4381 gcgctcgccg tgatcatggc cggcaccacg atgccgcggg agcgctacac ggagctcctg
     4441 gaggggctcg tcggcgggct gggacgatcc gcatcgcgca taacgggaaa ggcgcggctg
     4501 atgctcgtgg gaagcgcgaa cgacgacgtc gagctcgtgt gcgcgatcga gggcgcgagc
     4561 gcgatcgtgg tcgccgacaa cctgtgcttc gggtcgcgct ttcatgccga ccgcgtctcc
     4621 gaggacgggg acccgatcga ggcgctggcg cggcgctatc ttgccaaaaa cgagtgtccc
     4681 cgcgtatacg gcgagtacaa ggcgcgcctg gcgaccctgc ggcacaaggc ggagcaggcg
     4741 cgggtcgacg gggtggtgct gcagaacatc cgcttctgcg acctgcacgg atccgagaac
     4801 ggcctcttcg agcgcgatct cgaggccgca gggatcccct gcatccggct cgagcgcgag
     4861 tatgggccgc tcgtcgaaac cggacggatg aagatgcgca tcgacgcgtt tatcgagcga
     4921 atacgccagc ggaggaaaac ggcatgagac cggaaacccg ggaatatcaa ttcgactgga
     4981 tgctgtggag ctgcttctcg gcggcctcga agctgaacgc cggcaccccg aaggaatacc
     5041 gccggatgct ggagtacatc ccctcattcc ggggcgtggt ggagacggtg ctggacgcgg
     5101 gcgagccggg acagcgcttc atcgagatct gcgcgaccta cctcgacaac gtcatcaacg
     5161 cgaaatcgcg cgggcaaaag accgccatca caaccttctg cttttcgccg gccatcttct
     5221 acgcgatgga catcatgccc atctgcctcg aggtgatatc ggtgatgatg accttcacct
     5281 acaagcgcgg caccacggaa ttcctggact actgcaacga ggccggctac accgagacct
     5341 cctgctcgtc gcagcggggg agcatcgggg cctaccttgc gggactcggc gcggagatcg
     5401 acatgatcgt gaccgacacg ccgggcgtgt gcgacaccaa cgcgaacgcg ttcgccttcg
     5461 cggcggccta cctggacaag cccttctttc agctcgacat gccgcccgac ctcacgggcg
     5521 agcgctccaa cgaatatcac cgcgcggact acacggcgct catctcgttt cttgagcagc
     5581 acagcgggaa gcggctcgac ctcgaacggc tgcgggaaaa attgatcgag gtgcagaagc
     5641 aggacgagat gatcgccgag ctcgaggagc tggcgcgcat ggtccccaat ccgcttcccg
     5701 tcgtgtacaa cttcatggtc tatgcgtcgc gctttctctt cgccgggatg cccgagtgca
     5761 cgcggacgct cgaatcgctc ctcgcgctgg cgcgacagaa cgccgccgag gggaagtcgg
     5821 ggatctccgg cggcgcggag cgcctgcgat catacttttg ctacatagac cactacgtgc
     5881 agaatctcaa gctctggcaa atgctcgatg cgaacgggat ctgctaccag gggaacatcc
     5941 tctcgcgctc gtgggcgtcc aatgcgccgc atgtgcgcga gttcgggacg caggaggcgg
     6001 cctacacgct cgacaccgcg aacctcgacg ggctcatcga caccatggcc atgctcaact
     6061 cgcgcatgcc catgatcaaa tcgatacgcg gtccctacga tgcgcccaac atgtggctcc
     6121 aggacacgct ctcgctggcg acgatgtaca acgtcgactt catcgtctac aacggcacgc
     6181 cgggatgccg gaacacctgg ggcatggtga agctgttggc gcgcgacacc gagaaggcgg
     6241 gctttccgac gttcatcatg tacgccgacg ccttcgacga gcgcgtccag tcgtgggaga
     6301 cgacgcggga ccgtttcatg gaatttctca acgtgcggag gctgctatga cctgtgttgc
     6361 gggatgcgac gtgggatcgc tcacgtccaa ggcggtgatc atggacggcg accggatcgt
     6421 acagtcggtc atcatgaaat cgaaggcgaa gccgtgggag tcggccgacg aggtgatgcg
     6481 gcaggcgctc gagaaggcgg gcatgtcgat gggcgatctc gcctactgcg tgggcaccgg
     6541 ctacgggcgc gacaagatga ccttcatcaa cgaggccgtc tccgagatcg cctgccacgg
     6601 caagggggcg cagtggcttg tccccacggc gcggaccgtc atcgacatcg gcgggcagga
     6661 ctgcaaggcc gtgaagctcg atgatgcggg aaacatggtg aagttcatca ccaacgacaa
     6721 gtgcgcctcg ggcaccggcc gctttctcga ggtgatggcg aaggtgctgg gcgtggagct
     6781 cgcggacctc ggggcgctct cggcgaaatc gaagagtccc gtcaccctcg cctcggcctg
     6841 cacggtctgg gcgcaggcgg acgtgatcca gcacctgaac gacacggtgc cgctcgagga
     6901 catcgccgcc ggcgtgaacg ccgcgatggc aagccgcatg gcggtgctgg cgaacaatat
     6961 cggcgttgcg cgcgacgtgt gcatgaccgg cggcgtggca aaaaacgagg gcgtggtcgc
     7021 gtcgctggaa aaggcgctgg gcgtgcgcat caagcggatc cggaaatccg atccgcagct
     7081 cgccggcgcc atcggcgcgg cgctgtttgc gaaggaaaaa gtggacggag gcatgcgatg
     7141 atagcggccg gatgcgacgt gggctccctt accgcgaagg cggtgatcct gaaggacggc
     7201 gcgatcgccg cgcgcgaggt gatgcgggcc gccgcctcgc cggagcgctc ggcccgtgag
     7261 gtgatggagc gcgccgtcgc gcatgcgggc gtctcgatgg cggacatcgc gcgctgcgtg
     7321 ggcacgggct acgggcgcaa gcacatcgat ttcgtgcagt cgatggagtc ggagatcgcg
     7381 tgccatgcgc gcggcgcggt gtggcaggtc ccctcggccc gcacggtggt ggacatcggc
     7441 gggcaggacg cgaaggccat tcgcatcgac gaaacgggga acgtcgagcg ctatgcgtac
     7501 aatgacaagt gcgcgtcggg cacgggacgc ttcctggaga tcatcgccga cgcgctggag
     7561 atccccctcg atgagatggg ggccgtttcg cagaagtcga cggagcagct gaccctctcg
     7621 aatcaatgcg tcgtgttcgc cgagacagag atcatctcgc tggtgagcga ggggcgggag
     7681 atcgcggaca tcgtgagcgc gctgcatcgc gcggtcgcca accgcgcggc gtcgctcgcg
     7741 cgcggcatcg gcgtggccgg agacgccgtg atgacggggg gcgtcgcgaa gaacgccggg
     7801 atgtttctcg cgctcgagcg ggccctcggc gtcccgctga agagcgtccc cgatccgcag
     7861 atcaacgggg cgctgggcgc ggcgctcttc gcgcaggacg cgctggcgaa atgaacctct
     7921 tcgacgacag cgtcggccgg ctccggacct tcctcgagcg gaaacagtcc gccggagaat
     7981 tgcgcacaac gcaacttccc gcgacgacgg gctggcccgc gggggagcgg ggcaacatcg
     8041 tcctggccgc cgatacgggc gtcgagctcg ggaacccgcg ggtggaatcg gcctcgttca
     8101 tggtgtggac ggaagcggcg gatgcggtcc gggacggcgc ggtgcatgtc gtcggtcccg
     8161 accttgcgga gtcggcgggc cggagccttc cgttcggcaa ggcggtgatt ctgcgtgtgg
     8221 acggcatgaa cgaggagaac tgctacgagc gccaccgggc gcttgaactg atacggtacc
     8281 gactcaacct gaagggatac atgatgcgcg cggtctcgca gtatctgcgc gagtggagcc
     8341 gggtgagcgt cgaggcgatc gacggcggtt tttcgttcgc gacgctcgcc gggacgctcc
     8401 attcgatgta ccgcgcggtc gagtacgtat cggccgtcga gttcgtcttc gtcacctcgt
     8461 cgccggagga cgtgcgcgcg ctcggcgaga cgggcgcgcg cgtcgagcgc ctcatcggcg
     8521 cgatgaacaa gatggcgcag gagatgtcct tcgactgcgg cgtctgcgat tataccgacg
     8581 tgtgttccga ggtggaggac ctgcgcagca tgagaaagaa cgcggccgaa ggaggggcgc
     8641 atgcaacgag gcactgaggg acggacgcag gcgatcatcg ccctgtgcgg gaagggcggc
     8701 gtcgggaaga cggccctctc cgcgtcgatt atccgggcgc tcgtgcgcga tccggaaaag
     8761 cgggtgctcg cgatcgacgc cgatccagcc gtggggctcg ccacggcgct tgcgatcgag
     8821 gtgcgccgga ccgtcgacga ggtgcgcacc gatttcatcg agctggtgaa acagcgcgcg
     8881 gcgggcgaca aggaacgcgt gctctccctc ctcgactacg agatgctggg cgcgctgcac
     8941 gaagcgggcg ccctcgcctt catggccatc ggccgcccgg aaacggaggg ctgctactgc
     9001 caggtgaacg acatgctcaa ggacatcatc gcctcaatcg cgggcaactt cgattacgtg
     9061 gtcatcgacg ccgaggcggg cgtggagcag gtgaaccggc gcgtcctgga acgcgtgacc
     9121 catctggtgc tggtgagcga cctctcggcc aaggggctgc gcgtcgcgga gaccattcat
     9181 gatgtcgcgc ataacgcgat cgggttcgag cgcgccgggc tcatcgtcaa tcgcctgcgc
     9241 gacggcgcgg aactcgacgc cgtgccgctg ccggcgtcac ttccgttcct cggggccgtc
     9301 cccgaggacg agtgcatccg ccgcgccgac atcgaggcgc gcagctttct cgaccttccc
     9361 gacgcgccgg cctttgcggc gctcgcggag tgcctcgata aactggggat cgacgcgggc
     9421 ggccgggccg cctgcacggc cgggaggcac agcgcatgac ggcacccatg accgggctcg
     9481 cgggactctg cgcggatgtg cgcaatgaat tgatcgagga ggcgatgggg cgcgggatga
     9541 tcccgctggg ctacacctgc agcatggtgc cccgcgcgct ccttgccgtc ggaacgctct
     9601 tcccggtgcg gatgcgcgcg cccggcgtcg cgggcacgga gtgtgcggac gtgtacctga
     9661 gcagcgtgat ctgctcgtac agccgcagcc tgctcgagtt cgctctggac gggcgctatg
     9721 attttctggg cggatgggtc ttcgtcgcct gctgcgacca cctgcgccgc ctgtgcgaca
     9781 atttggaata caatgcgaaa ccggatttca gccataccct cgatattccg caccggggag
     9841 ggccggcggc gctggaatgg accgtcgagg agctgcggag gctcgcgcgg gcgatcgaac
     9901 ggcatttcga cgtggacatg ggcgacgacg cggtccgcgc ggccatggtc gcggaaggcg
     9961 agctcgtcga tgcgctccgg gagctcgggg cgctccgcaa aaaaccgcag ccgccctttt
    10021 cggggaccga ctatcatcgc gtgttgatgg cggcgcagag tgctcccccg gcgcgcgccc
    10081 tggagctggt gcgcgagttc cgcgaggcca tgggcgacgc ttccgatacg gccccctacc
    10141 gcgcccgctt cctcctcgtg gggggctcgc tggatgatcc ggcctacacg gcggtcatcg
    10201 aatcccaggg cggcctcgtc gtggccgacc gctactgcac gggatcgtat ccgtggctcg
    10261 atcccgtcga cgcgggcggg gacccgttcg tcgccctggc gcgccaggtg ctcgacggga
    10321 accgctgccc gcgcatgatg gagggtttcg cgcaacgcgt caactatatc gtcgatctcg
    10381 cccgcgagta cgcggccgac ggcgtcatca tacagtcgat aaagttttgc gatacgtggg
    10441 gcgtcgaggc gagcgccctg gtgaccgcac tgcgcgaggc gggcgtgccg gtcctgcgcc
    10501 tggagcggga gtaccgccag agcggggagg ggcagctccg gacgcgggtg caggcgttta
    10561 ttgaaagcat ggggaaatga catgggcact ccgggaatcg ccgattatgc cgtaacctcg
    10621 aaatacaagc tcctcaacct cgccgtcatt gcgctcatgg gtcttcggaa cggtccctac
    10681 gacatggtgc gcgacctgcg gcgctatccg tggctgtggc acctgctcaa ggtgaaccgc
    10741 ctcatcgaca agctcaccgc cgggcgcacc gggaattacc gcgaggcgat ctcgatcatc
    10801 gtccacggcg tcaccaagga ggtggtcgat cttctggacg ggatctactt ccggcaggac
    10861 cggctcgtgc tccacgagga catggtgccg ccggagatcc tgcgcgccat ggggctctcg
    10921 ccgtggatgc ccgaggtgct cgggatcctt ctgcccatgc tcgagccctc cgcgatggag
    10981 gagtacatcg acgtcgcgga gaacgagggc gtgccggccg acgtgtgcag cctgcccaag
    11041 gcggtgatcg gtacggcgtt gcgcgacatg ctaccgcccg cgcgcgcggt ggtcacctcc
    11101 aatctcccct gcgacggcgg gatgacctcg tacctctacg tggagaagat gaccgcggcc
    11161 ccgaccttcc ggctggacat ccccttcaat ttcaaggacg agcgcgcgga cgcctatttc
    11221 gcgaacgagc tccggcggat gatcgcctgg ctcgaggagc atacgcccgg ccgcatcgac
    11281 tgggaccgcc tgaaggagat ctgcgaggag cgcaaccgca tggtggagca ggagctggag
    11341 ctgtgggaca tgatcgcact ccggccggcg ccgctggcgg ccgaggccgt gttcctgggg
    11401 catctctggt tcatgaacgt gtgctccggg gtgccggcgg ccaccgaggt gttccgccgg
    11461 ctcgtggaac tcgcgaaaga gaacctcgcg cgcggggtgg cggcgtgtcc cgacgagcgg
    11521 taccgaacgc tcctgtggaa cccgcccacc atgcacttca tcgacctgtt cacctgggcc
    11581 gagcgcgcct acggcgtgac ctgcatcatg gacagcatga gcttcaaccg gctgcccttc
    11641 atcgacacct cgagcgagga gtcgatgctg cgcgggatcg ggcgaaacat catgaacggg
    11701 ccgatggcgc ggcatacgcg cgggcccatg gcgaactact tcgacgacat gttccacatc
    11761 atcaagcgct tcgatatcga catgctgtgg atggcggggc acatcggctg caagaacacg
    11821 caggcgctca acgccatcat gcgcgagaag tgccgcgacg tgggcgtgcc ggtgctcatc
    11881 atcgactacg atctttcgga cacgcgcatc gtgccccggg agggcatcat gacgcaggtc
    11941 gacagcttta tggagaacgt gatgcatgcg aagcggcgcg cgtgacctca cctcacccgc
    12001 cctccgggcc gccctcttcc gtaggagagg gcttcggtgg atacgcccct ttctaaatac
    12061 tcccccttct cctatgggag aaaggggccg gggggatgag gttgagggcg cgaccatctt
    12121 taaatgaaag taataggtac aaacaatgaa aaagaaattt ttcgcggggt gcgacatcgg
    12181 atcaaccacc ggcaaggcgg tgatcatcga cgagaacggc gtggtcgcct cgtcgatcgt
    12241 gccgcgcgag gtcgatcccg aggagacggc gcatctcgcg ctcgccgagg cgttcaaggc
    12301 catccccgat gtgaacacgc actcggacct cgcctatctc gtgggcaccg gctatgggcg
    12361 cagtgaaatc cccttcgcga atgaaaacgt ctcggagatc agctgtcacg cgatgggcgc
    12421 gtattactgc gatcccgcca tcaagaccat cgtagacatc ggcggtcagg acgtgaaggg
    12481 catcgccgtc aacggcgacg gcagcgtgaa ggacttcgcc atgaacgaca agtgcgcggc
    12541 cgggaccggg cgcttcttcg aggccatggc ccgcatcttc cgcatggagc tctccgagtt
    12601 ctcggacctc tcgctcgcgt cgaagcgggc cattcccgtg acggcgcagt gcagcgtctt
    12661 cgccgagacc gaggtgatca gcctcctcgc gcagaaaaac ccgcccgccg atatcgccgc
    12721 cggcgtgcag ggggccgtcg ccaagcgctg cttcacgctc ctcaagcgcg tgggcgtaca
    12781 cccgacggtg accatcaccg gcgggtgcgc gaaaaatcgc ggcctggtcc gcgagctcga
    12841 gcgggtgctg cgcgtcgaga tggcgtcgct gtcagtggac ccgcagatca tcggcgcgct
    12901 tggcgccgcg gtgtacgcga aaaaaaaggc gcggggctga ttccgcttcg cgtcgtttcg
    12961 tacgccgtac cccgattcgc gctccgtgcg gcatcaccat tttcctgttg acgttcccgc
    13021 cgcgttatgc caccatcacg ataattgttc aataacaacc tcatccgcgg gtccgtcgag
    13081 cgcgtatggg tttcctgcct acgcttggcg ctgcggcatc ctgttggatg tctgctcacg
    13141 ggcgggaatg ggtgagcgaa tagtgaattg ctggtgatcc ttcttcttcg ggaaacggca
    13201 ttgtgtacag cttgatgcgt tactgcatgt atgacattct atcgcaatga gcgtgcagct
    13261 gttcatcgag atgtgtacat cgagtataat cgaggggaag gtgggacaaa aatgatcaaa
    13321 ataattaaga gcctgtttca gtatgtgtat tgccaatagc accgaatgtg acggtaggat
    13381 gtcacagcga gtaagcgacc cggtttggtt gccggagccg cgaatacatg gatgtattgt
    13441 tcgcggcgga gcgagcttac atcctaccat cacattcgac tccctatgtg aataggttaa
    13501 aataatgagc gggtatttgc catgaaaaag cattccatcg tcgccctaat cgggtgtatc
    13561 atctttctcg tcggctgcaa aaccagttat gaatacatca tcaccgggaa gcagtatccg
    13621 ccgcttaaaa aagacgaccc ggtcaagatg gtcttcctcg aagacaaagg gcgctacgag
    13681 gagctgggca tcgtcgaggt gactgggaag acgctcatga agcgcgttga cgccgcggtc
    13741 gacgttgcgc gcaagcaggg cggcgatgtg ctcatgataa agggagtgcg cgacgagaag
    13801 gcgatggagg agctacgatc gcaggaggac ccgtcccggc cgctcaagca atcatttctc
    13861 gtgctgcgca cccggccgga cgacccgaat gagcgcttgg acgacgagga cgcgcccgcc
    13921 gtccgttccg gcggccccaa aaagtccgtg aaagcgccgt cgatccgtac gggcgtggac
    13981 gacgagggcg cgctcgacga agccgatccc tccggcctca agcgggcgac ctggcgactg
    14041 ctggtggaag agttcgagat gctgcgcggc gagcgcttcc gggcgtccat gtatccgctc
    14101 gcctcatcgc ggaagatacc accttcgatc gattatcacg cggatggcgc gaagcgactc
    14161 atcaccatgg ccacgcgggg ccgtcgccat ttcgcatatc tcatcgtgcc aaaaaagatg
    14221 gagcagcgca tcatgcgcgc gaagcgcttc aagcggtcgt tcgaattcgt gtatacgccc
    14281 gttgacgtgt atcgcagggg agggaaaaca taccccgtgt tggagtttgt tcgtgagatc
    14341 gaaggcgcag ggagcaagta aggtgagtcg tcaggcggtg cggtttttcg ggtgaaagca
    14401 gtatgttgca ttagcagggt agatagtttg ggcaccctat taacccacta cagcatatcc
    14461 gaaattcgct ttcatgaccg tattcaattc gtaaacgaat tacgggtatc acgactctgt
    14521 ggtataaatt gcgaaagtat tttcttttat gaatgagtta agtgaaacgg ccaacaaact
    14581 taaggggcgg gcatccgtgc ccttatagtt ggaaataaaa tttaacgttt taggagatac
    14641 attatgttaa aaaaacttgt ttgcttggtg attattttat ttg
//
LOCUS       DAORHF010000077        14565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3207_length_14565_cov_5.628325, whole genome shotgun sequence.
ACCESSION   DAORHF010000077 DAORHF010000000
VERSION     DAORHF010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14565
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3207_length_14565_cov_5.628325"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="PKJ16_08240"
     CDS             <1..714
                     /locus_tag="PKJ16_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47015.1"
                     /translation="LAIDTAGRTFADRARGATVRDPDGGCDIRVGAGGLRTNAWLAVR
                     PIRDASIRHGVAARLGIGAAGIVRLFSVAHDDAVCAQPAVVSLKRPPKVSDAGSGRYV
                     VCSWYDGGLPRSHPTRYDPRADAFVAQTKTLGMLALVRDDAPPDVVLPPTHELFEDRD
                     GARFVRVCLADRISGLDHGSIVCLVDGEPLAVSYDRDRMWIEIELPREHLARGVHHLL
                     VRCRDRAGNEGILRSLVAF"
     gene            complement(711..5894)
                     /locus_tag="PKJ16_08245"
     CDS             complement(711..5894)
                     /locus_tag="PKJ16_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47016.1"
                     /translation="MYQIGAPVGQFRIIEAIAESYKSELYRAEHAGGTVRLKALKSVR
                     RVPAVRAQIRQEYAAIRDINCAGVVRALDLIEDNDTVVFVHEDYEGVPLLSHLRGAPI
                     GLDALLRIAARTATVLGEVHRRNVVHRDIKPHNIIITGDLDTDPTVLVTDFGIGVILD
                     GEIQDIYNLNVIKGRLPYLSPEQTGRMNRPVDYRTDLYSLGIVLYELATGTVPFRSTD
                     PLVIIHSHMAKDPVDPARINPELPPVVSRIILRLLAKGPEDRYQNALGLAHDLEECIA
                     QRARAGRIADFTLCTRDISERFNLPQKIFGRQREIAALLEAFGRVSEGSRELVLVSGP
                     PGIGKTMLIHEIHKPIVSRHGYFFSGKFDQLRRDVPYSSVIQAFQSLVRQILAEGSER
                     VDRWRQRVVEAVDGDIGLITALIPELEHLVGAHDDPPALAGEEALIHFQAAFRRFTGV
                     FARAEHPLAVFLDDMQWADPGSLALVGALASDPELSHLFFILSYRNTEVGESHPLMNL
                     VSAAHAAGVRINALEVPPLSTSDVNDFAANFLRRSDERTTELASLVHRKTGGNPFFIN
                     QFLKTLYEERYIRFDPARGWSWDIGVINAMMVSDNVVEFMLDRIARLPEDARQVLKIC
                     ACIGNRFELETVAVIIDRSLDETLSLLGIALNETLISAESSLYRFFHDRIQEAAYAML
                     TPAEKGRLHYRIGRLALERTGPDELRDRIFYIVDHLNYDPSIASDTRERRDIARLNLI
                     AGRKSSASAAYDAALHYYGAGISLLDDAAWSEAYDLAIALHVAAGEACYLTGAHGRMD
                     SLFQTAFSHARSPFDLARAHELLINRFTVQHRHREALAHGLAVLAEMGLRMPARAGAA
                     TLLAALVPVRLRLLFRRDERIMNAAPMSDERVSAITRILMACSEPCYLGDPAYLPLVI
                     LRLVSYTLRYGTAPHAAYAFVAYGVIISQFFGDSAGGYRFGELAMRLQERSGAPGLRG
                     RINFLFGGMIIHWRRPLADSLPILLDGYRSAIDSGDLSFASYNLTNYLYNALYASTPL
                     PELIVRYGRHLEDIRKFERLIGTTDFALWHQLVCALMQTPESAGDAAGHLYHDPSIAA
                     GWREAKHGSILAVYGPGRLALLYCTGRYDDALREARILAPYQRNVLGQSFYPEFHFLY
                     ALAACAAAPSADRAALATARTVGRRHRKWAHLAPGNYLHRFLLIQGELSRVTGSRRRD
                     ERGTRRTASELYERALDMARRSGHTRDSAIVCECAGRHHLRRGNRALASLYLAAAAGA
                     YAAWGAASIADNVRTRFGGLLAHEARPSTPEGARTLRPDARSVDLATIMKTSQALTSE
                     IDLGNLLRQILEFAIENAGASYGALILEDPSAGDPPRLVIRAQGGVHEPIRTMLEEPV
                     DTGAAVSASVVNYVYRTRDTVILGDAGAEGMFATDPVIIMRRAKSVLCAPIVYQQKLS
                     GIIYLENSLAPDVFTPERLELLGMLSAHAAISIENALLLDERERTARLRREMEIAANI
                     QRMLLPAAPALDGFEIAAHMQPAENVGGDYYDIITERGVTWIIIGDVSGHGLNSGLVM
                     MMVQTAIHVLLRDDPFIMPDELLTTVNRTVSDNLNKISDSKYMTITALRYDADGAFVY
                     SGLHLDIIVRRAATRAVEAVKTDGRWIGFPDEIYPAEVRAANNVLRLSPGDTMLLYTD
                     GVTDAIVSDDRRLNIEGLMDIVRAWDGSGPRALCDAIVGAVRACRTHDDVTLLAVRRL
                     P"
     gene            5994..7667
                     /gene="ilvB"
                     /locus_tag="PKJ16_08250"
     CDS             5994..7667
                     /gene="ilvB"
                     /locus_tag="PKJ16_08250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HNV47017.1"
                     /translation="MKGAEIIIECLKKEGVEYMFGYPGGVVIPIFHHIFNAPFTFILT
                     RHEQGAVHAADGYARASGKVGVVIATSGPGATNLVTGIATANMDSVPLVCITGQVSTD
                     LIGNDAFQEADITGITRSITKHNYLVQDVKDLARIIKEAFYIASTGRPGPVVIDVPVD
                     ISNADTEFVYPDKVDIRSYKPVYQGHPVQIARACRLIEQAKRPIVYAGGGVIISNASG
                     ELRDFVHKTGIPITTTLLGLGTFPETDPLSLQMLGMHGTYFANHAVHQSDLLIAVGAR
                     FDDRVTGKIDEFIPHAKVIHIDIDPASVSKNVKVDVPIVGDCRHVLAAMTKEVNPPEI
                     GEWVAHIADMRDKHPLTYDDRDGVIKPQYVIEQIYELTKGDAVITTEVGQNQMWAAQF
                     YTYTEPRSFISSGGLGTMGFGFPAAIGAQLARPDKRVIDIAGDGSIQMNIQELIVAVQ
                     HRLPVIIAILNNGFLGMVRQWQQLFFDRRYSNTCISFAPDFVKLAEAYGAAGIRVEQK
                     TEVAPAIERALSITDRPIIIDFRVEREENVYPMVPRGKSVKETIMRELA"
     gene            7664..8146
                     /gene="ilvN"
                     /locus_tag="PKJ16_08255"
     CDS             7664..8146
                     /gene="ilvN"
                     /locus_tag="PKJ16_08255"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HNV47018.1"
                     /translation="MSKHVISVKVENRFGVLARVAGLFSARGYNIDSLTVSETEDESI
                     SLMTIVVRGDDRIIEQVKKQLNKLIDTIKVIDHPAGSSVQRELALVKVNAQPQKRSEI
                     YQIAEIFKAEIVDISKKTVTLEATGDPEKIDTIIELLRPYTIRELIRTGRVSLLKEKD
                     "
     gene            complement(8196..8630)
                     /locus_tag="PKJ16_08260"
     CDS             complement(8196..8630)
                     /locus_tag="PKJ16_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47019.1"
                     /translation="MDTSIHIRTDVMSQISRAAHAKGIPRSRLITQLLYAVMKHAPER
                     LRTGRLVKYQQRAAPDDWRVFHITLSEDDYEYVLDLRKIMKMSLSHIVAYAVSRYLDT
                     GTIAFGTDNYRFNNYVGIRETYDNCISWRFIWGYQKTMRRRH"
     gene            8908..9903
                     /gene="ilvC"
                     /locus_tag="PKJ16_08265"
     CDS             8908..9903
                     /gene="ilvC"
                     /locus_tag="PKJ16_08265"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007223015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HNV47020.1"
                     /translation="MAKMYYDSDADLGALKDKTIAVIGYGSQGHAQAQNLRESGLTVI
                     VAELPGTENFELAKSHKFAPVSAKEAAEKADVIQVLAQDHIQAKLYYSDVEPSLKKGK
                     TLVFSHGFNIHFGQIAPPKDVDVIMVAPKGPGHLVRSEYAKGAGVPSLIAVHNDASGK
                     AKQTALAYAKGIGATRAGVIETTFKEETETDLFGEQCVLCGGAAELVRAGFDTLVAAG
                     YQPEIAYFECLHELKLIVDLFYQGGINYMRYSVSDTAEYGDYVVGKRIINENTRAEMR
                     KVLAEVQDGTFAKNWILENMAGRPQFNMTKKMQSEHHIEKVGSELRKMMKWIDAK"
     gene            9957..10940
                     /locus_tag="PKJ16_08270"
     CDS             9957..10940
                     /locus_tag="PKJ16_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV47021.1"
                     /translation="MEEITLGEKIALLTKVGVFSKLKNEEIAVVATYSRMMDVPRGTA
                     VFTENSYGRQLFVVKEGEVLITKHREREHDIDLAQYLPGESFGEMDLVANMRRGANAV
                     AIRDTRLLVFPAKGITFEEVLTKYPRISAQILSKMLATVAGRVRRTHELIREREPWIQ
                     NLKKQLFVDTLTGLLNANFLREDLPGQLPFFEEPVSLILVKPDNFKKFNDAYGHEAGD
                     RALMLVAVILQSVERESYIAARYRGDEFVLVLPGTGREEAIDVARDLGRSLYEVDFRD
                     ITCGAPFKLALSAGIALYPQHARDGRALLEIAYEKMLGARNKGGNRIAAAG"
     gene            complement(10941..12170)
                     /locus_tag="PKJ16_08275"
     CDS             complement(10941..12170)
                     /locus_tag="PKJ16_08275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV47022.1"
                     /translation="MIRHKERAYGSPAASGWRIIAALAVLIAAIGGNAIAASSDGEFH
                     ALMDRAQNEIKAADWTSLVRTAERLCALRTDNIWANYYRGLALTNSGRSAEGEECLKK
                     AIAIDNTVFWPHYMLFFNYFNRGRPEMHAHARNVMQRITPALIQSNPRDIRIFYVVYA
                     GSLINGTDMNGAQAVLQKGIGFFPKDPILRSQYAFTFFRRDMKTWERLSRETLSLIPH
                     EEQTARGTYALPLRGSMIKVHQGNNETISHLGLLNGYDWDFVRVDERGAYGRNGAKKE
                     GHAIFGAIVYAAADGVVYFVEDSSPDTEPMRNHPTAAPNRIIIQHPNGEMTSYIHLMK
                     GSVRVKPGDAVKRGQPIGRVGSSGSTVDIPHLHFGVSRDSHSVEAKITGFELLTNGSW
                     SDPGPRVLRKNDIVRRK"
     gene            complement(12268..12762)
                     /locus_tag="PKJ16_08280"
     CDS             complement(12268..12762)
                     /locus_tag="PKJ16_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47023.1"
                     /translation="MTIRYITCCLLIVACAPMAYASLDPLIGEREFRAASATLAQELG
                     AHIKKTHTAPPLIGLTPIRNATSEHIDTGLFASLLTRELLTYGIKVRNERHRADHDAK
                     PSADPAALLLSGEIRGIWAVDGENRVCTTFIILTLTDAKTRMILWTKDVSFRRIVPKR
                     SLSF"
     gene            complement(12837..>14565)
                     /gene="polA"
                     /locus_tag="PKJ16_08285"
     CDS             complement(12837..>14565)
                     /gene="polA"
                     /locus_tag="PKJ16_08285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HNV47024.1"
                     /translation="VSVDTETTSVNPVDAELVGISLSIAPNAGWYVPVACDSLFSEAI
                     LDRDAALEVLRPMLEDPAVRKIGQNIKYDMIVLAMAGIEIAGVSFDTMVASYLLAPGE
                     RRHNMDDLAAQYLNYTTITYAELVGKGKNAVPITAVPLDRLSEYAIEDADITLRLRNV
                     LAPLLDANDLRTLFETIEMPLVRVLAVMERNGVKIDTAHFARLAQENEGLLRDAEEKV
                     YAAAEIRFNINSTRELADVLFNRLRLKTVKKTKTGYSTDISVLEALQGAHPIIDHLIA
                     YRTLSKIRNTYIDTLPQLVSPNTGRIHTSYNQTVVATGRLSSSDPNLQNIPVRDDYSR
                     KIRAGFVPEKGRLILSADYSQIELRIAAHISNDANMIRAFRDGIDIHSMTAAAVFGVP
                     IDGVLPEMRRQAKIINFATIYGVSPFGLSQQADIGIKEAALFIQKYLDNYPGIREYIE
                     RTIAFAREHGYVQTMLGRRRPVPDIHADVSFRREGAERIAINTPIQGTSADLIKIAMI
                     RIQRLLEEKRLGAKMIIQVHDELVFEVPEREKDEIESLVRREMEAALALSVPVVVDMG
                     WGAHWAEAH"
BASE COUNT         2664 a         4357 c         4875 g         2669 t
ORIGIN      
        1 ctggcgatcg acacggcggg acgaacgttt gccgatcgcg cgcgcggagc gacggtgcgc
       61 gatccggacg gtgggtgcga tatccgcgtg ggggcgggcg gccttcgaac gaacgcctgg
      121 cttgcggtgc gtccaatccg cgatgcgtcg attcgccacg gcgtggccgc acggctcggg
      181 atcggggcgg cgggtattgt tcgcctgttt tccgtcgcgc acgatgatgc ggtgtgcgcg
      241 caaccggccg tcgtgtcgtt gaagcggccg ccgaaggtct ccgatgcggg gagcgggcga
      301 tatgtcgtgt gcagctggta cgacggcggc cttccccggt cgcacccgac gcggtatgat
      361 ccgcgcgccg acgccttcgt cgcgcagaca aagacgcttg gcatgcttgc gctggtgcgc
      421 gacgacgcgc cgcccgacgt ggtgttgccg ccaacgcacg agctcttcga ggaccgggac
      481 ggcgcgcgct tcgtgcgggt gtgcctggcc gatcggatct cagggctgga ccacggatcc
      541 atcgtttgtc tcgtcgacgg cgagcccctt gccgtttcgt acgatcggga ccggatgtgg
      601 atcgaaatcg agctgccgcg ggagcacctt gcgcgcggcg tccatcatct gctcgtccgc
      661 tgccgggatc gcgccgggaa cgagggaatc ctgcgctcgc ttgtggcctt ttagggaagc
      721 cgccgcaccg cgagcagggt cacatcgtcg tgggtccggc aggcgcggac cgcaccgacg
      781 atcgcgtcgc acagcgcgcg cggcccgctt ccatcccatg cgcgcacgat gtccataagc
      841 ccttcgatgt tcagccgccg gtcgtcggag acgatcgcgt ccgtgacccc gtcggtgtac
      901 agcagcatgg tgtcgcccgg ggacagccgc aggacattgt tcgcggcgcg gacctcggcg
      961 ggataaatct cgtcgggaaa gccgatccag cggccgtcgg tcttcacggc ctcgacggcg
     1021 cgcgtggccg cccggcgcac gatgatgtcc aggtggagcc cggaatacac gaaggcgccg
     1081 tcggcgtcgt agcgcagcgc cgttatcgtc atgtatttgc tgtccgatat tttgttgagg
     1141 ttgtcgctca ccgtccggtt cacggtggtc aggagctcgt cgggcatgat gaacggatcg
     1201 tcgcgaagca gcacgtggat ggcggtctgc accatcatca tcaccaggcc ggaattgagg
     1261 ccgtgccccg aaacgtcccc gatgatgatc caggtgaccc cccgctccgt gatgatgtcg
     1321 tagtagtcgc cgccgacgtt ttccgccggc tgcatgtgcg cggcgatctc gaagccgtcg
     1381 agcgcgggcg cggcgggcag gagcatgcgc tggatgttcg cggcgatctc catctcgcgc
     1441 ctgaggcgcg ccgttcgctc ccgctcgtcc aggagcagcg cattttcgat ggagatggcg
     1501 gcgtgtgcgg acagcatgcc gagcagctcg agccgttccg gcgtgaatac gtcgggcgcg
     1561 aggctgtttt ccagatagat gatccccgag agcttttgct ggtagacgat gggcgcgcac
     1621 aggaccgatt tcgcgcggcg catgatgatg acggggtcgg tcgcaaacat gccctccgcg
     1681 ccggcgtcgc cgaggatgac cgtgtcgcgt gtccggtaca cgtagttcac cacggaggcg
     1741 gagaccgccg cgcccgtgtc caccggctcc tcgagcatgg tcctgatcgg ttcgtgcacg
     1801 ccgccctgcg cgcggatgac gaggcgcggg ggatcgcccg cggacgggtc ctcgaggatg
     1861 agcgcgccgt aggatgcgcc cgcattttcg atggcgaact ccaggatttg gcggagcagg
     1921 ttccccaggt cgatttcgct ggtgagggcc tgcgaggtct tcatgattgt cgccagatcg
     1981 acgctgcgcg catccgggcg aagcgtgcgc gccccttcgg gggttgatgg ccgcgcttca
     2041 tgcgcgagca ggccgccgaa gcgcgttcgc acgttgtccg caatcgacgc ggcgccccag
     2101 gcggcgtagg cgccggcggc cgccgcgaga tagagcgacg cgagggcgcg gttgccgcgc
     2161 cgcaggtgat gacgccccgc gcactcgcag acgatggccg aatcgcgcgt gtggccggac
     2221 cggcgcgcca tgtcgagggc gcgctcatag agctcggagg cggtgcgccg ggtcccgcgc
     2281 tcgtcccgcc ggcgcgaccc cgtgacgcgg gacagctcgc cctggatgag cagaaaacgg
     2341 tgcaggtagt tcccgggggc caggtgcgcc cattttcgat ggcggcgccc cactgtccgg
     2401 gccgtggcga gcgcggcgcg gtcggcggac ggagcggcgg cgcacgcggc gagcgcatac
     2461 agaaaatgaa attccggata aaacgattgt cccagcacgt tgcgctggta cggcgcgaga
     2521 atcctcgcct cgcgcagcgc gtcatcatac cgcccggtgc aatacaggag cgcgaggcgt
     2581 ccgggcccgt atacggcgag aatggaccca tgcttcgcct cgcgccagcc ggccgcgatc
     2641 gacgggtcgt ggtacaggtg tcccgcggcg tcgccggcgc tttccggggt ttgcatcagg
     2701 gcgcatacca gctggtgcca gagcgcgaaa tcggttgtcc cgatgagccg ctcgaatttc
     2761 cgtatgtctt cgaggtggcg gccgtatcga acgatgagtt ccggaagcgg cgtggaggcg
     2821 tagagcgcat tgtaaaggta attggtcagg ttgtacgatg cgaacgaaag gtcgccgctg
     2881 tcgatggcgc tgcgatagcc gtcgagcaga atggggagcg aatcggcgag cgggcgcctc
     2941 cagtggataa tcatgccgcc aaaaagaaag ttgatgcggc cgcgaagccc gggggcgccc
     3001 gatcgctcct gcagccgcat ggcgagttcg ccgaaccggt atccgcccgc gctgtccccg
     3061 aaaaactgtg agatgatgac gccgtacgcg acaaaggcgt atgcggcatg gggagccgtg
     3121 ccgtagcgca gcgtatacga gaccaaccgg aggatcacga gcgggagata ggccggatcg
     3181 cccaggtaac agggctcgga gcaggccatg agaattcgcg taatggcgga aacgcgctcg
     3241 tcgctcatcg gggccgcgtt catgatccgc tcgtcgcgcc tgaacagcaa gcgcagccga
     3301 accgggacga gcgcggccag aagggtcgcc gcgcccgcgc gcgctggcat ccgcagcccc
     3361 atctccgcaa ggaccgccag cccgtgcgcg agcgcctcgc ggtgccggtg ctgcaccgtg
     3421 aaccggttga taagtaactc gtgtgcgcgc gcgaggtcga acggcgagcg ggcgtgcgaa
     3481 aacgcggtct ggaagaggga gtccatccgg ccgtgcgcgc cggtaagata gcacgcctcg
     3541 ccggcggcca cgtggagggc gatcgcgagg tcgtatgcct cgctccaagc ggcgtcgtcc
     3601 agaagcgaga tccccgcgcc atagtagtgg agcgcggcgt cgtaggcggc cgaggcgctc
     3661 gatttgcgcc ccgcgatgag gttgagccgc gcgatgtccc tgcgctcgcg cgtgtcggag
     3721 gcgatcgacg ggtcgtaatt gagatggtcc acgatgtaaa agatgcggtc gcgcagctcg
     3781 tcggggcccg tccgctccag cgccagacgc ccgatgcggt aatgcagccg gcccttctcc
     3841 gccggggtga gcatggcgta ggccgcctcc tggatgcgat cgtggaagaa gcgatagagc
     3901 gagctctcgg cgctaatcag ggtttcgttt aacgcgattc ccagcaggga cagggtctcg
     3961 tcgagggagc ggtcgatgat cacggccacg gtctccaact cgaagcggtt gccgatgcac
     4021 gcgcagatct tgagcacctg ccgggcgtcc tcggggagcc gcgcgatgcg gtcgagcatg
     4081 aactcgacga cattgtcgct gaccatcatg gcgttgatga cgccgatgtc ccacgaccaa
     4141 ccccgcgcgg gatcgaacct gatatagcgc tcctcgtaga gcgttttcag gaactggttg
     4201 atgaagaacg ggttcccgcc ggtcttccgg tggaccaggg aggccaactc ggtcgtgcgc
     4261 tcgtcgctgc gccgcaggaa attcgcggcg aagtcgttca cgtcactcgt cgagagcggc
     4321 ggcacctcga gggcgttgat ccgcacgccg gcggcgtgcg ctgcggagac caggttcatc
     4381 agggggtggg actcgccgac ctcggtgttt cggtacgata ggatgaagaa gaggtgcgag
     4441 agctccggat cggacgcgag cgcgccgaca agcgcgagac tgccgggatc ggcccattgc
     4501 atgtcgtcca ggaagaccgc cagggggtgc tcggcgcgcg cgaacacgcc cgtgaatcga
     4561 cgaaacgccg cctggaagtg aatgagcgcc tcctcgccgg cgagggccgg cggatcgtcg
     4621 tgcgcgccca ccaggtgctc gagctcgggg atgagcgccg tgatgagccc gatatcgcca
     4681 tcgaccgcct cgaccacgcg ctgccgccac cggtcgacgc gctcgctgcc ctcggcgagg
     4741 atctgccgga cgagcgactg aaaggcctgg atgaccgagc tgtagggaac gtcgcgacgg
     4801 agctggtcga actttcccga gaagaaatac ccatgacgcg agacgatcgg cttatgtatc
     4861 tcgtggatga gcatggtttt tccgatgccg ggcggaccgg agacaagcac gagctcgcga
     4921 gacccttcgc tcacgcggcc gaaggcctcc aggagcgcgg cgatctcccg ctggcggccg
     4981 aagatcttct gcgggagatt gaaccgctct gagatgtcgc gcgtgcacag ggtgaagtcg
     5041 gcgatccgcc cggcccgggc gcgttgtgcg atgcactcct cgaggtcgtg cgcgagtccc
     5101 agcgcgttct ggtaccggtc ctccggcccc ttcgccagga gccgcaggat gatgcgcgac
     5161 acgaccggtg ggagctcggg gttgatccgc gcggggtcca cgggatcttt cgccatgtgt
     5221 gaatggatga tgaccagcgg atcggtcgat cggaacggga cggtcccggt ggcgagctcg
     5281 tagaggacga tgcccagtga gtacaggtcg gtgcggtagt cgaccgggcg gttcatgcgg
     5341 ccggtttgtt ccggagagag atacgggagg cgccccttga tcacgttcag gttgtagatg
     5401 tcctgaatct cgccgtcgag gatcactccg atgccgaagt cggtcaccag aactgtcggg
     5461 tcggtgtcga gatcgccggt gatgatgatg ttgtgcggct tgatgtcgcg gtgaacgacg
     5521 tttcgccggt ggacctcgcc gagaacggtc gcggtccgcg cggcgatgcg caggagcgcg
     5581 tcgagcccga ttggcgcgcc ccgcagatgc gacagcagcg ggaccccctc gtagtcctcg
     5641 tggacgaaca cgaccgtgtc attgtcctcg ataaggtcga gcgcgcggac caccccggcg
     5701 cagttgatgt cgcggatggc ggcgtattcc tgccgtattt gcgcccgtac ggcggggacg
     5761 cggcggacgc tcttgagcgc cttgaggcgg accgtgccgc cggcgtgctc ggcgcgatag
     5821 agctccgatt tgtagctctc cgcgatggct tcgatgatcc ggaattgacc gaccggcgct
     5881 ccgatctggt acatgggccg cggcgtcctt tgcaaaaaaa atatgttgac agtgccgatt
     5941 gcgataataa tactccaacg ctatttttca atgaattttt acagcgagag catatgaagg
     6001 gcgcagagat catcatcgag tgtttaaaga aagaaggcgt tgaatacatg ttcgggtatc
     6061 ccggcggcgt ggtgattccc atttttcatc acatcttcaa tgcgccgttc acgttcatcc
     6121 tcacgcgtca cgagcagggc gcggtgcacg ccgccgacgg ttacgcgcgc gcatcgggca
     6181 aggtgggcgt ggtgattgcg acctccggtc ccggcgcgac caacctggtc acgggcatcg
     6241 cgacggccaa catggactcg gttccgctgg tgtgcatcac cggccaggtg tcaaccgacc
     6301 tgatcggcaa tgacgccttc caggaggccg atatcacggg cattacgcgc tcgatcacca
     6361 agcacaatta tcttgtgcag gacgtgaagg atctggcgcg aatcatcaag gaggccttct
     6421 atatcgcatc gaccggacgg ccgggaccgg ttgttatcga cgtgcccgtc gacatatcga
     6481 acgccgatac cgagttcgtg tacccggaca aggtcgacat caggagctac aagccggtgt
     6541 accagggcca tccggtccaa atcgcgcgcg cgtgccggct catcgagcag gcgaagcggc
     6601 ccatcgtgta tgccggaggc ggcgtgatca tctccaatgc gtccggcgag ctgcgcgact
     6661 tcgtgcacaa gacgggcatt cccatcacca cgacgctgtt gggattgggc acgttccccg
     6721 agaccgaccc gctatcgctg cagatgttgg ggatgcacgg aacctatttt gcgaaccatg
     6781 ccgtgcacca gagcgatctg ttgatcgcgg tgggcgcgcg cttcgacgac cgcgtgaccg
     6841 gcaagataga cgagtttatc ccgcacgcga aggtgatcca catcgacatc gatcccgcgt
     6901 cggtgagcaa gaacgtgaag gtggacgttc ccatcgtcgg cgattgccgg catgtgctcg
     6961 ccgccatgac gaaagaggtg aaccccccgg agatcgggga gtgggtcgcg cacatcgccg
     7021 atatgcgcga caaacacccg ctcacctatg acgatcgcga tggcgtgatc aagccgcagt
     7081 acgtgatcga acagatttac gagctgacga agggcgacgc cgttatcacc accgaggtgg
     7141 ggcagaacca gatgtgggcg gcgcagttct atacgtacac ggagccgcgt tcgttcatct
     7201 cgtctggggg gctcgggacg atgggcttcg gctttcccgc ggccatcggc gcgcagctcg
     7261 cgcggcccga caagcgcgtg atcgacatcg ccggcgacgg ttcgatccag atgaacatcc
     7321 aggagctcat cgtcgcggtg cagcaccggc tgccggtgat catcgccata ctgaacaacg
     7381 ggtttctggg gatggtgcgc cagtggcagc agcttttctt cgaccgccgc tactcgaaca
     7441 cctgcatcag ttttgcgccc gatttcgtga agctcgccga ggcctatggc gcggcgggca
     7501 tccgggtcga gcagaaaacg gaggtggcgc ccgcgatcga gcgcgcgttg tcgatcacgg
     7561 accggcccat tatcatcgac ttccgcgtgg agcgcgagga gaacgtgtat cccatggtcc
     7621 cgcgcggcaa atcggtgaag gagaccatca tgagggaact cgcatgagca agcacgtcat
     7681 ctccgtgaag gttgagaacc gttttggcgt gctcgcgcgc gtggccgggc tcttctccgc
     7741 tcgcgggtat aacatcgatt cgctcaccgt gagcgagacg gaggacgagt cgatatccct
     7801 gatgaccatc gtggtccgcg gagacgaccg catcatcgag caggtgaaga agcagctcaa
     7861 caagctgatc gacaccatca aggtgattga tcaccctgcg ggctcgagcg tgcagcgaga
     7921 gcttgcgctt gtgaaggtga acgcgcaacc gcagaagcgt tccgagatct atcagatcgc
     7981 cgagatattc aaggcggaga tcgtggacat ttcgaagaag accgtcacgc tcgaggcgac
     8041 cggtgacccg gagaaaatcg acaccatcat cgagctgctg cggccgtaca ccatccggga
     8101 gctcattcgg acggggcgcg tgtcgctttt gaaggaaaag gactgacgcc cgggccgcgg
     8161 acgggtttgg tgaccgatag ttatcggtcg tccgctcaat ggcgccggcg catggtcttt
     8221 tggtagcccc agatgaatct ccaactgatg cagttgtcgt aggtttccct gatgcccacg
     8281 taattgttga accgatagtt atcggtgcca aacgcaattg tgccagtatc gagatatcga
     8341 ctgaccgcgt atgccacgat atgcgagagc gacatcttca taatcttccg taggtcgaga
     8401 acgtactcgt aatcatcctc gctgagtgta atatggaaaa cgcgccagtc atcgggcgcg
     8461 gcccgctgct ggtatttgac cagccgtccc gtccggagtc tctcgggagc gtgtttcatg
     8521 acagcgtaca gcagttgcgt aatcagccgc gagcgcggaa tccccttcgc gtgagctgcg
     8581 cgactaatct gcgacataac gtcggttctg atgtggatgg aggtgtccat ggtcgtctcg
     8641 ctgcaatcga tatcggcgtc tatatatagt acgaacgcgc ggcgagatgt gtgacatttt
     8701 atggggaaat ggggactcgg agtatgtgtg gtggtaggat gcttcgagaa ttatttacca
     8761 cgtatatggc gaagaagacg ccgatgcgcc gggataacca taaaacaaca gtttagtccg
     8821 agaaaaaatg tttgtaaaaa gaatgtcgat tatatgtaac ggcacacaat catatgcgat
     8881 attacaaata taaggagaaa tcatatcatg gcaaagatgt attacgatag cgacgccgat
     8941 ctcggcgcgc tcaaagataa aaccatcgcg gtcatcgggt acggcagcca ggggcacgcc
     9001 caggcgcaaa acctccgcga gagcggactc acggtgatcg tggccgagct tcccggcacg
     9061 gaaaatttcg agctcgcgaa atcgcacaag ttcgcgccgg tatccgcaaa ggaggccgcc
     9121 gagaaggccg atgtgatcca ggtcctcgcg caggaccaca tccaggcgaa gctgtattac
     9181 agcgacgttg agccgtcgtt gaaaaagggg aagaccctgg tgttctcgca cgggtttaac
     9241 atccacttcg ggcaaatcgc gccgccaaag gacgtcgatg tgatcatggt cgcgcccaaa
     9301 gggccgggac acctcgtgcg cagcgaatac gcgaaggggg cgggcgtccc ctcgctcatc
     9361 gcggtgcaca acgacgcgtc cgggaaggcg aagcagacgg cgctcgccta tgcgaagggc
     9421 atcggcgcaa cgcgcgcggg cgtcatcgag accacgttca aggaagagac cgagaccgac
     9481 ctcttcggag agcagtgcgt gctgtgcggc ggggccgccg agctcgtgcg tgcgggattc
     9541 gacacgctgg tcgcggcggg ctatcagccc gagatcgcct acttcgagtg cctgcacgag
     9601 ctcaagctca tcgtggacct cttctatcag ggcgggatca attacatgcg ctattcggtg
     9661 tccgatacgg ccgagtacgg cgactatgtg gtcgggaagc gcatcatcaa cgaaaacacc
     9721 cgcgcggaga tgcggaaggt cctcgccgag gtccaggacg gcacgttcgc caaaaactgg
     9781 atcctcgaga acatggccgg ccggccccag tttaacatga ccaagaagat gcagagcgaa
     9841 catcacatcg agaaggtggg aagcgagctt cgaaagatga tgaagtggat cgacgcgaag
     9901 tagtcgccat gcacgggccg gtggggtgcg cgccggcccc gtgctgcacg tgaattatgg
     9961 aagagatcac cctcggtgaa aagatcgctc tcttgacgaa ggtgggcgtt ttctccaagc
    10021 tcaagaacga ggagatcgcg gtcgtcgcca cctatagccg catgatggac gttccgcggg
    10081 gaacggccgt gttcaccgaa aactcctacg ggcgccagct cttcgtcgtc aaggagggcg
    10141 aggtgctcat caccaagcac cgcgagcgcg aacacgacat cgacctggcc cagtatctgc
    10201 cgggggagtc gttcggcgag atggacctgg tggcgaacat gcggcggggc gcgaacgccg
    10261 ttgcgatccg cgatacgcgg ctcctggtgt ttcccgcgaa gggcatcacc ttcgaggagg
    10321 tgctcaccaa gtacccgcgc atctcggcgc aaatcctttc caagatgctg gcgaccgtcg
    10381 ccgggcgcgt gcgcaggacc cacgagctta tccgcgagcg cgagccctgg atacagaacc
    10441 ttaaaaagca gctcttcgtc gacaccctca cgggcctgct caatgcgaac tttctgcgcg
    10501 aggaccttcc gggccagctc ccctttttcg aggagccggt gagcctcatc ctcgtcaagc
    10561 cggacaactt caagaagttc aacgacgcct acgggcacga ggcgggggac cgcgcgctca
    10621 tgctggtcgc ggtgatcctg cagtcggtcg agcgggagtc gtatatcgcg gcgcgctatc
    10681 ggggcgacga gttcgtgttg gtgctgcccg ggaccggccg cgaggaggcg atcgacgttg
    10741 cccgcgatct cggacgatcc ctctacgagg tcgattttcg cgatatcacc tgcggcgcgc
    10801 cgttcaagct cgcgctcagc gccggtatcg ccctgtaccc ccagcatgcc cgcgacggca
    10861 gggcgctcct cgagatcgcc tacgaaaaga tgctgggcgc gcgcaacaag ggcggaaacc
    10921 gcatcgcggc cgcgggatga tcacttccgt cggacgatgt cattcttccg taacaccctc
    10981 ggcccgggat cgctccacga tccgttcgtc aggagctcga acccggtgat cttcgcctcg
    11041 accgagtgac tgtcgcgtga aacgccgaaa tgcaggtgtg ggatgtcaac cgtgctcccg
    11101 ctcgagccga ccctgccgat cggctggccg cgcttcaccg cgtcgcccgg cttcacccgc
    11161 accgatccct tcatgagatg gatgtaggag gtcatctcgc cgttcgggtg ttgtatgatg
    11221 atgcggttcg gcgcggcggt cggatggttc ctcattggct cggtgtccgg gctcgagtcc
    11281 tcgacgaaat acacgacgcc atcggctgcg gcgtacacga tcgccccgaa aatcgcgtgc
    11341 ccctcctttt tcgctccgtt gcggccatag gcgccgcgtt cgtccactct cacgaaatcc
    11401 caatcgtacc cattgaggag tcccaggtgc gaaatggtct cgttattgcc ctgatgcacc
    11461 ttgatcatcg aaccccgcag gggcagcgcg taggtgcccc gggccgtctg ctcctcgtgc
    11521 gggatgagcg atagcgtctc acgggagagg cgctcccacg tcttcatgtc gcgccggaaa
    11581 aaggtgaatg cgtactggga gcgcaggatg ggatcttttg gaaaaaaacc gatccctttt
    11641 tgtaatacgg cctgcgcgcc attcatgtcc gttccattga tgagagagcc ggcataaacg
    11701 acataaaaaa tcctgatgtc gcgcggattc gactgaatga gcgccggcgt tatgcgctgc
    11761 atgacgttcc gcgcgtgcgc atgcatctcc ggacggccgc gattgaaata attgaagaag
    11821 agcatgtagt gcggccagaa gaccgtattg tcgatggcga tagccttctt gaggcactcc
    11881 tcgccctcgg cggagcgccc gctgttggtg agcgcgagcc cacggtagta gtttgcccag
    11941 atattgtcgg tgcgtagagc gcacagccgc tcggcggtac gcacgagcga tgtccagtcg
    12001 gctgctttaa tctcattctg cgcccggtcc atcaatgcgt gaaactcgcc gtcgctcgat
    12061 gcggcaatcg cgttcccgcc aatcgcagct atcaatacgg cgagcgccgc gatgattcgc
    12121 cacccgctcg cagccggcga tccgtacgcc cgttccttgt gtcgtatcat ggaagttgtc
    12181 ctctgtcggg atttttctag aatggtgaat cgtaaacaac gtaatgtgtg tcgcgcatgt
    12241 cccatcacgc ttgccgcgcg agaccagtta aaatgaaagc gatcgcttcg ggacgattcg
    12301 gcgaaaggac acgtcctttg tccagaggat catcctggtt ttcgcgtcgg tgagcgtaag
    12361 gatgatgaag gtcgtacaga cgcggttctc gccgtcgacg gcccagatgc ccctgatttc
    12421 gcccgataga agcagcgcgg ccggatcggc tgatggcttc gcgtcgtgat ctgcgcgatg
    12481 ccgttcgttc cggaccttga ttccgtacgt caggagctcg cgtgttaata gcgacgcgaa
    12541 caggccggtg tcgatatgct ctgaggtcgc attgcggatc ggcgtcaatc cgatgagggg
    12601 cggggccgta tgcgttttct tgatgtgcgc gccaagctcc tgcgccagcg tggcgctggc
    12661 cgcgcggaat tcccgttcgc cgatcagcgg atcgaggctc gcgtacgcca tgggtgcgca
    12721 cgcgacgatg agaagacagc aggtgatgta tcgtatggtc atgacggcct ccgttgtgat
    12781 gcatgatggc gcgccgctcc agcgctcgtg ctgatcgggc gtgggacgag cccccctcag
    12841 tgcgcctccg cccagtgcgc gccccatccc atgtccacca cgacgggaac ggagagcgcg
    12901 agcgcggcct ccatctcgcg acggacgagc gattcgattt cgtctttttc ccgctccggg
    12961 acctcgaaaa ccagctcgtc gtgcacctgg atgatcatct tcgcgccaag gcgcttctcc
    13021 tcgaggaggc gctggatgcg gatcatcgcg atcttgatca ggtcggcgga ggtgccctgg
    13081 atgggcgtgt tgatggcgat ccgctccgcg ccctcgcggc ggaacgatac gtcggcgtgt
    13141 atgtccggca cgggccgccg ccgcccgagc atcgtctgga cgtagccgtg ctcgcgcgcg
    13201 aaggcgatgg tgcgctcgat gtactccctg atgcccggat agttatccag gtacttctgg
    13261 atgaacagcg ccgcctcctt gatgccgata tcggcctgct gcgagagccc gaagggcgag
    13321 acgccgtaga tcgtcgcgaa gttgatgatc ttcgcctggc ggcgcatctc gggcagcacc
    13381 ccgtcgatcg gcacgccgaa gacggccgca gcggtcatgc tgtggatgtc gataccgtcg
    13441 cgaaaggcgc ggatcatgtt cgcgtcgttc gagatgtgcg ccgcgatccg aagctcgatc
    13501 tgcgagtagt cggccgagag gatgaggcgg cccttctcgg ggacgaaacc ggcgcggatc
    13561 ttccggctgt agtcgtcgcg caccgggatg ttctggaggt tggggtcgga tgaggagagg
    13621 cggcccgtcg cgaccacggt ctggttgtac gaggtgtgga tgcgtcccgt gttcggcgag
    13681 acaagctgcg ggagcgtgtc gatgtaggtg ttgcggatct tcgagagcgt ccggtacgcg
    13741 atcaggtggt cgatgatggg atgcgcgccc tgcagggcct ccagaaccga gatgtcggtg
    13801 gaatagccgg tcttggtctt cttgacggtc ttgaggcgga gccggttgaa gagaacgtcg
    13861 gcaagctcgc gcgtagagtt gatgttgaag cggatctccg ccgcggcgta caccttctcc
    13921 tctgcgtcgc gcaggagccc ctcgttctcc tgggccagcc gcgcgaagtg cgcggtgtcg
    13981 atcttcacgc cgtttcgctc catcaccgcc agcacgcgca ccaggggcat ctcgatcgtc
    14041 tcgaagagcg tccggaggtc gttcgcgtcg agcagcggcg cgagaacgtt gcgcaggcgc
    14101 agggtgatgt cggcgtcctc gatggcgtac tccgacaggc ggtccagcgg cacggcggtg
    14161 atgggaacgg cgtttttgcc ctttcccacg agctcggcgt aggtgatcgt cgtgtagttg
    14221 agatactgcg cggcgaggtc gtccatgttg tgccgccgct cgccgggcgc gaggaggtac
    14281 gaggcgacca tggtgtcgaa ggagacgccc gcgatctcga tccccgccat cgccaggacg
    14341 atcatgtcgt acttgatgtt ctggccgatc tttcggacgg cggggtcctc gagcatcggt
    14401 cggagaacct ccagcgccgc gtcgcgatcg aggatcgcct cggaaaaaag cgagtcgcac
    14461 gcgaccggga cgtaccagcc cgcgttgggc gcaatcgaaa gggagatccc gacgagctcg
    14521 gcgtcgaccg gattcaccga cgtggtctcc gtgtccaccg agacc
//
LOCUS       DAORHF010000078        14545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3213_length_14545_cov_5.000621, whole genome shotgun sequence.
ACCESSION   DAORHF010000078 DAORHF010000000
VERSION     DAORHF010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3213_length_14545_cov_5.000621"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..654
                     /locus_tag="PKJ16_08290"
     CDS             157..654
                     /locus_tag="PKJ16_08290"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNV47025.1"
                     /translation="MTTANKTNRPAMSIAAAGILVGLAVSGIALFMAPPCSVATAIGW
                     NFLFLRKASWETIHIVFAVAFVIVVVCHLRYNWGTLTGHFARGTRTAGGISRSALIAI
                     IIAVVLFVLSALQLPPVSWVYDAHERIKFSHSPGGGQGQGGGGGNRGRGMRRQASDRG
                     MGETR"
     gene            820..1545
                     /gene="prxU"
                     /locus_tag="PKJ16_08295"
     CDS             820..1545
                     /gene="prxU"
                     /locus_tag="PKJ16_08295"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083760882.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:1072..1074,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HNV47026.1"
                     /translation="MPEDFKPGCARPTGGPVGDTPVIIEPESASARKEEQKPMIQVGK
                     KAPDFTAPAYIQGKFANVTLSEYLGKWTLLCFYPGDFTFVUATEISAVAEKYPEFQKL
                     GVQVLSMSVDSVFVHKMWNDNELSKMVTGGVPFPMLSDSGGRVGTTYGIYDADAGVET
                     RGRFIIDPDGIIQGFEVLTPPVGRNVSESIRQVQAFQLVRESKGAQATPSGWKPGKMT
                     LKPGPDLVGKVWEVWKTSMAFDE"
     gene            1770..2240
                     /gene="bfr"
                     /locus_tag="PKJ16_08300"
     CDS             1770..2240
                     /gene="bfr"
                     /locus_tag="PKJ16_08300"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HNV47027.1"
                     /translation="MKGNAKLIDTLNGLLADELTAINQYMVHSEMCDDWGYEKLHKHF
                     EKRAIDEMKHAEKLIGRILFLEGTPTVTTLNKISIGAAVPAQFANDHAAEEGAIKAYN
                     DAIAFAGEVRDFATRDLLQQILNDEDRHMDEIEALQGQIEQMTIQVFLTTQVKE"
     gene            2424..3392
                     /gene="hemB"
                     /locus_tag="PKJ16_08305"
     CDS             2424..3392
                     /gene="hemB"
                     /locus_tag="PKJ16_08305"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HNV47028.1"
                     /translation="MLIQRPRRLRGSALMRDAVAETTVSAKRLVMPHFVCEGSGVREP
                     IGAMPGIDRVSIDNLARDVERDHACGVDAIILFGIPAVKDEKASQAYAADGIVQRAVR
                     EIKRAAPGVLVITDVCLCEYMSHGHCGVVRDGVIVNDASCELIAKTALSHAQAGADIV
                     APSDMMDGRVAAIRTTLDACGRCDTPILSYAVKYASAFYGPFREAAGSAPQFGDRRSY
                     QMDFRNAREAEKEARLDVDEGADIVMVKPALAYLDIVRRVREAVSVPVCAYNVSGEYS
                     MVKAAAAAGRVDERSIVIEILTAIVRAGADMVITYHARDIARERWL"
     gene            3413..4708
                     /gene="hemL"
                     /locus_tag="PKJ16_08310"
     CDS             3413..4708
                     /gene="hemL"
                     /locus_tag="PKJ16_08310"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HNV47029.1"
                     /translation="MNIARSIELYARASALMPGGVNSPVRAFKSVGGTPLFIAKGTGS
                     RVYDEDGNAYIDYCMSWGPLLLGHADDDVVRAIADAARDGTSFGTPNRREVEIAELVI
                     ARFPAVDKVRFVNSGTEAVMSAIRLARGFTGRDKIVKFDGCYHGHADSMLVAAGSGLA
                     TFGTPDSAGVTEGAARDTIVVPFNDIDALRAVMDRDHGSIAAVIMEPVPCNYGLIPPR
                     DGYLRAVRELCDRFGALLIFDEVITGLRLAPGGAQERFDVRPDITTLGKIIGGGLPVG
                     AYGASTEIMAKVAPDGPVYQAGTLSGNPLAMAAGIATMKKIDALNAYDLLEEKGRRFD
                     ALVAPIADEYRGRALILRLGSIVCTYFTERTSIESVDQVRACDMKRFARYHAEMLRRG
                     IYLAPSGYEVGFLATAHTDDDLADTAAAMRESLAAAFGS"
     gene            complement(4799..5605)
                     /locus_tag="PKJ16_08315"
     CDS             complement(4799..5605)
                     /locus_tag="PKJ16_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47030.1"
                     /translation="MKKLLFLAIILALSAANAHAAFEFEKQSGITVVQKNEGEEETEL
                     LLKDARGNAFTVVFSDKISDNMGKLILTLKDDFYNFKTVKIKDVKFAVGRDSLDANLT
                     PAQLEFTGQNLLPYITSGLLFTYRNDLRYNFRIAAPDKNNREKMVAVRIRGEFMTEDR
                     LCAKIAEAVKDPVYFNKKRDPEYLIGKIEELEKTQERVRYAVMTLHNTGFFSGPAPVQ
                     FSVIKRVIELKAGNPKLSKQQLVEALEKENIKTSGKEVSVVLAVFFNEFE"
     gene            complement(5610..6797)
                     /locus_tag="PKJ16_08320"
     CDS             complement(5610..6797)
                     /locus_tag="PKJ16_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47031.1"
                     /translation="MGNENEIKDLVQSKSAFLPEEIQDLSAARHVYSLDDEFSKTKKN
                     RSLLFPFIVLAFMSVLVLAAVGITKYTEWKQQKAVLSITEFDDLKLGELLSGAKKFQG
                     QLKNAQTELESLRKKMDEEMSAAGSPSERNKIRSKYQGRIGSKEREIASLEKEMQSYD
                     FKLEQTVKKAEEMVNNYKRLHELKMNEQRDQIILKYNPKYDNARLNAVIHASTSPAVK
                     RGDPSKLYQQLAAKKLITAAGAEKLKRGVDNQLLIMDRLLQIPYVNSVRPSLAQMHHL
                     MKSSANDYETLVTALFSLTQRQEAAIRGYQYALEQYGRQNRESGFIVDARDSDSIRVY
                     VSKVLKVRSGDRAMVFREDDQYIGTIEFYMAGGEIRAKRVELAGINTFHPFDKILIKQ
                     QGE"
     gene            complement(6776..8482)
                     /locus_tag="PKJ16_08325"
     CDS             complement(6776..8482)
                     /locus_tag="PKJ16_08325"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47032.1"
                     /translation="MGKLLDNLFGQKINKARVFPVTLKIIIVFTLFILVSNLSSNYIN
                     LIYNRTELIGLMRNLLTKDLKDANTFCNNQYEIFQYTKDLNEAVAGIENKGVRDFTKK
                     KSVLLGVKPDGTLLFQGTKIKKAEKFADAQALKQMADNQVRTIQEGFINFRYNNEDYF
                     GAYKHNANWSVYIVRAEELNEFYEESRNIFIRISVIILLVTLISAVVGIFLLRFILRY
                     IPIITSGIMKMVSNQQLELIDLSKAPNDDITYLGVAFNSLSNTINNLVNIFRKFTSRD
                     IAIKAYKERDVRLEGNQRDLAILFTDIKSFTFITETLGADIIKLLNLHYDQAIREIMR
                     LDGVIGSIIGDALLAVFGVFEESTENKSLQAVTSAYKVQEVAESLRARMHERKEQIMK
                     EKGALSRSESRVFKAVLLEVGVGIDGGNVFYGTIGSYERMTNTVIGDNVNAASRLEGL
                     TRIYKVPVICSDYVRKDIVDNVSSHGIRFVELDQVMVKGKTTAKKVYWPLIESTITPA
                     MEKNLENYSAGLKAYYNGDWKKAYLLFKKCSLPLAEEFVERTKTFKSPKNWNGVWEMK
                     TK"
     gene            8688..8927
                     /locus_tag="PKJ16_08330"
     CDS             8688..8927
                     /locus_tag="PKJ16_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012465682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47033.1"
                     /translation="MKTTEYLRFIRMRTDRRCMRDTWTQGVIEHPIHEVVQPDGRIRR
                     WGVIAEAHGRYPRVIVLPDGETVHDAFFDRCLRAA"
     gene            8929..9135
                     /locus_tag="PKJ16_08335"
     CDS             8929..9135
                     /locus_tag="PKJ16_08335"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HNV47034.1"
                     /translation="MIIKHFSDTDTLYVQFSNRDSIETRDLDRNTVIDLDANGELVAM
                     TIEHATETASMHEFSFQSKSQSAY"
     gene            complement(9197..10663)
                     /locus_tag="PKJ16_08340"
     CDS             complement(9197..10663)
                     /locus_tag="PKJ16_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HNV47035.1"
                     /translation="MSAQYHIKTFDTITTLRRFIWLIVPVVSIAGFFQPKLGLFIFPL
                     FAAIMVMGFFTGRYWCGNFCPRGALFDFPVRAIGAFDRIPRLFTSAWFRALVLALLMG
                     FFIRNTVNAFSAWGTVSFWDRLGMVGVMMCAATTGIGLVLSLVVNPRTWCSFCPMGTV
                     QKLLHAARTALIGPVFANPRITMTQPSACQKCGKCARVCPMQLAPYRELNDVDQVAAL
                     DCIKCGSCVAHCAGNVLALESERKTDTYRLPEPLPGYADRAAADAVIESVEEIGTDIR
                     EFTFRLRDGKTLTPKPGQFVAVRVDDENEVFRSYSVSRPTDDPSRVKIAVKLDPNGWG
                     TPRLFAMREGDTVRLEGPMGDFTVRDTDTDKLFIAGGIGITPFLSLAREAARKHPGRT
                     VRLLYGANAGRDVIYAQYLEELRREHPNFDYAIALARPDDGWQGHRGMVTDLIDAVTT
                     ENAEAYLCGARPMIGAATRSLAGRGMPAEAIHVDTAFS"
     gene            10915..12051
                     /locus_tag="PKJ16_08345"
     CDS             10915..12051
                     /locus_tag="PKJ16_08345"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47036.1"
                     /translation="MNNERYLLGAINADLGKKMVFIGGPRQVGKTYLSKRLLDLNASD
                     QYLNWDSSAHRKIILGKTWDRTKALVVFDELHKYRPWKSWLKGVFDTEERPPAVLVTG
                     SARLNVFRRGGDSMMGRYFYFRLHPFSLRELVEYCDWPPETALAALWERGGFPEPLFS
                     ESALDAARWRKSRLETIIREDALTLENVRHLASLELLVDLLRERVGSPISYKALSEDL
                     GVSPQTVKSWIDLLERLYLVFSVTSYHRSLARALKKEIKVYFYDYSDIGDGGARFENL
                     IASHLMKEAHYLEDVQGRRVSLHTLKDKEKREVDFLVAENGRPIELVEAKLGRDSFSA
                     PLMYYARRFPGIAATQVVKDLPESRSRDGVRMVGARDFLTGRGV"
     gene            complement(12429..12932)
                     /locus_tag="PKJ16_08350"
     CDS             complement(12429..12932)
                     /locus_tag="PKJ16_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47037.1"
                     /translation="MREFKAEMKDFRDDGARQIKEMNKKWGELSNKMGTLVEDIIFPG
                     LPRVIKDTYGLETVDLSIRRKKTLPGGRNREFDAIAVAGEYVFVNSTKGTMRKNYIDD
                     FIVEMGEFREFFPEYKDHKLVGLLASLYVDPGLTAYGERCGFYVLGLGDSPIAVLNAP
                     GFRAKEW"
     gene            13462..14511
                     /locus_tag="PKJ16_08355"
     CDS             13462..14511
                     /locus_tag="PKJ16_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47038.1"
                     /translation="MNTIYGRRVGETLVGLLSCMVMAFASCSGSGGGDGGGGGGGGAA
                     GSAGVSTLAVKGSLGAAFAMNKPMKWYDRIIALVSFSGNLYAGPDSVDKIVAVPVDHS
                     FSSNENLIESAITIDINPDKSFTINLDRTKRWVLLLVNSTASWKNRVVAFAALGDTNN
                     LVLLPLDNATSNIELGNLSKSGSDAVESGSLIGNTASFSTNISKLREVAQMSCMTKVI
                     KNMYQNHVRKLESGGEYYYSWTDHNWMVNSAGAENSFTNPSLYTYKGYHPTFRTEKYS
                     EISFSGIYDKDYTVSITAPAAVSPYGGGGPFTVFDNTKAWDGSRTRPGEYAVTLDATP
                     PKFAIMYFSRNYSAL"
BASE COUNT         2866 a         4043 c         4435 g         3201 t
ORIGIN      
        1 ttatacaggt aaatccacga ttgtgcaagc agggggtcac atcatattgt ctaacaaagg
       61 ggaaaggggc tgggggaaag gggtaaaaca aataattgcc aattacaatt gcagcttcgc
      121 tgtgcaatga agtgtttcac gaaactggag caaacgatga caactgccaa caagacgaac
      181 cgtcccgcga tgtcgattgc cgcagcgggc atcctcgttg gacttgccgt gtcgggaatt
      241 gcgctcttca tggcgccgcc gtgctcggtg gccactgcga tcggctggaa cttcctgttc
      301 ctgcggaagg cgagctggga gaccattcat atcgtgttcg cggtcgcctt cgttatcgtt
      361 gtggtttgcc atctgcgcta caactgggga acgcttaccg gccacttcgc gcgcgggacg
      421 cggacggcgg gcggcatctc gcggtccgct ctcatcgcga tcatcatcgc cgtcgtgctg
      481 ttcgtgctgt cggcgctgca gctaccgccg gtctcgtggg tatacgacgc ccacgagcgg
      541 atcaagttct cgcactcccc tggcggcggc cagggacagg ggggcggggg cggcaatcgc
      601 ggtcgcggca tgcggcgtca ggcaagcgac cgtggaatgg gagaaacccg ataaggccgt
      661 cgataaaaaa ttgatacgac ggcgcccggc tgatatgatc cgaataaaca tgacatgacg
      721 attcgcattt tccccgcctg actctgaagc aatcgctatc ggggcgaggc gcatggtaca
      781 ccaccacaac ggtcgtatac ttttttaaag gaggagccta tgcccgaaga tttcaaacca
      841 ggttgcgcgc gacccaccgg gggacccgtg ggcgacacac cggtcattat cgaaccggaa
      901 tcggcatcag cacgaaagga ggaacaaaaa cctatgatcc aggtcggcaa gaaagcgccc
      961 gatttcacgg cgcccgcata catccaggga aagttcgcaa atgtgaccct gtccgaatac
     1021 ctcgggaagt ggaccctgct ctgcttctat ccgggcgact tcacctttgt gtgagcgacc
     1081 gagatttcgg cggtcgccga aaagtatccc gagttccaga agctcggcgt gcaggtcctc
     1141 tcgatgagcg ttgacagcgt ctttgtccac aagatgtgga acgacaatga attatcaaaa
     1201 atggtcacgg gcggcgtgcc cttccccatg ctctccgaca gcggcgggcg ggtcggcacg
     1261 acctacggca tctatgacgc cgacgccggc gtggagacgc gcgggcgttt catcatcgac
     1321 ccggacggca ttatccaggg cttcgaggtg ctcacgccgc ccgttggacg caacgtatcc
     1381 gaatcgatac gccaggtgca ggccttccag ctcgtgcgcg agagcaaggg cgcccaggcg
     1441 accccctccg gctggaaacc cggcaagatg accctcaagc ccggccccga cctcgtgggc
     1501 aaggtctggg aggtctggaa gacgagcatg gccttcgacg agtgacgacc gccgccgggg
     1561 ggaggtcggc gaccgctccc cggcgcgcat gaacttggcc cgcgcgctcg ggagggattc
     1621 cataagaaga ttatcttcgc ctgtgcggaa aatatgcttt tctttttgat gcaggatgtc
     1681 atcatttctc aataacgatc atgcgggcga tcgaatacgc cgggacgcgc agtccccaat
     1741 actacatcta ttcacgcagg gaggaaatca tgaaaggtaa cgctaaactc atcgacacgc
     1801 tcaacgggct gctcgcggat gagctcacgg ccatcaacca gtacatggtg cattcggaga
     1861 tgtgcgacga ttggggctac gagaaactgc acaagcattt cgagaagcgc gccatcgacg
     1921 agatgaagca cgcggagaag ctcatcggac gtatcctctt tctcgaggga acgccgacgg
     1981 tcaccacgct caacaagatc agcatcggcg cggccgtccc cgcgcagttc gccaacgacc
     2041 acgcggccga agagggcgcc atcaaagcct acaatgacgc gatcgccttc gccggcgagg
     2101 tgagggattt cgcgacgcgc gatctcttgc agcagatcct caacgacgag gaccggcaca
     2161 tggacgagat cgaggcgctg cagggacaga tcgagcagat gacgatacag gtgttcctga
     2221 cgacgcaggt caaggaatag gctgatgcgg cggcccggcc gccgtcccgc cgatgtgatt
     2281 cctcgggccg catttcctcc ggaccaggag cgatgcggcc ttcgtgtctc atggcgcgcg
     2341 ggaaaccggg cattgcgcgc catatcttcc ttgacagcgg cccgtgcggg tgcggatgat
     2401 tgccgcgtgg agggtggacg cccatgctga tacaacgacc ccgccgattg cgcggctcgg
     2461 cgctcatgcg cgacgcggtc gccgagacga ccgtgagtgc gaagcggctc gtcatgccgc
     2521 acttcgtatg cgaggggagc ggcgtgcgcg agcccatcgg cgcgatgccg gggattgacc
     2581 gcgtgtcgat cgacaatctc gcgcgcgacg ttgagcgcga tcacgcgtgc ggcgtcgacg
     2641 cgatcatcct ctttggcatt cccgccgtga aggacgaaaa ggccagccag gcctatgccg
     2701 ccgacggcat tgtccagcgg gccgtgcgcg agatcaagcg cgccgcaccg ggcgtgctcg
     2761 ttatcaccga cgtgtgcctg tgcgagtaca tgagccacgg gcattgcggc gtcgtccggg
     2821 acggcgtgat cgtgaacgac gcgtcgtgtg aactcatcgc gaagaccgcg ctctcgcacg
     2881 cgcaggccgg cgccgacatc gtcgccccct cggacatgat ggacggccgc gtcgcggcga
     2941 tccgcacgac gcttgacgcc tgcggtcggt gcgacacgcc gatcctctcc tacgccgtga
     3001 agtacgcctc cgcgttctac gggccgtttc gcgaggcggc cgggagcgcg ccgcagttcg
     3061 gcgaccgccg gagctaccag atggatttcc gcaacgcccg cgaggcggag aaggaggcgc
     3121 gcctcgacgt ggatgagggg gccgacattg tcatggtgaa gccggcgctt gcgtatctgg
     3181 acatcgtccg ccgcgtgcgc gaggcggtgt cggtgccggt gtgcgcgtat aacgtgagcg
     3241 gcgaatactc gatggtgaag gcggccgcgg cggccggacg cgtcgacgag cggagcatcg
     3301 tgatcgaaat cctcaccgcg attgtccgcg ccggggccga tatggtcatc acctaccacg
     3361 cgcgcgacat tgcccgcgaa cgctggctct agcatacagt ggagaaaatt aaatgaacat
     3421 agcgcgttcg atcgaactct atgcgcgggc gtcggcgctc atgccgggcg gcgtgaactc
     3481 gccggtgcgc gccttcaagt ccgtcggcgg cactccgctg tttatcgcga agggaacggg
     3541 atcgcgcgtg tacgatgagg acggaaacgc gtatatcgac tattgcatgt cgtggggccc
     3601 gctcctgctc gggcacgcgg acgacgatgt ggtgcgcgcg attgccgatg cggcccgcga
     3661 cgggacgagc ttcggtacgc ccaaccgccg cgaggtggag atcgccgagc tcgtgattgc
     3721 gcgcttcccg gccgtcgaca aggttcgctt cgtgaactcg ggaaccgagg cggtgatgag
     3781 cgcgatccgc ctcgcccgcg gcttcacggg ccgggacaag atcgtcaagt tcgacggctg
     3841 ctatcatggg catgccgatt ccatgctggt cgcggcagga tccggactgg cgacctttgg
     3901 cacgcccgat tcggcaggcg taactgaggg cgccgcgcgc gacacgatcg tcgtcccctt
     3961 caatgacatc gatgcattgc gggcggtgat ggaccgcgat catggctcga tcgccgccgt
     4021 catcatggag ccggtcccct gcaattacgg gctcattccg ccgagggacg gataccttcg
     4081 agcggtgcgc gagctctgtg atcgcttcgg ggcgcttctc atcttcgacg aggtgatcac
     4141 gggacttcgc ctcgcgccgg gcggcgcgca ggagcgcttc gatgttcggc cggacatcac
     4201 cacgcttggg aagatcatcg gaggggggct tccggtgggc gcgtacgggg cgtcgacgga
     4261 aatcatggcg aaggtcgcac ccgacggtcc cgtctatcag gccggcacgc tttcggggaa
     4321 cccgctcgcg atggccgccg gcatcgccac gatgaaaaaa atcgacgccc tcaacgccta
     4381 cgacctcctt gaggaaaaag gccgccgctt cgatgcgctc gtcgcgccaa tcgccgacga
     4441 ataccgcggt cgcgcgttga tcctgcggct gggatctatc gtatgcacat atttcaccga
     4501 gcgtacgtcg atcgaatcgg tggaccaggt gcgcgcgtgc gacatgaagc gctttgcgcg
     4561 ctatcatgcc gagatgctgc ggcggggaat ctaccttgcg ccgtcgggat acgaggtggg
     4621 cttcctcgcc accgcgcaca cggacgacga ccttgcggat accgccgcgg cgatgcgtga
     4681 atcgctggcg gcggcgttcg gttcgtgatg ggccggatca acgaaaagag gccggacatg
     4741 acgcccggcc tccctggtgc tgcggtgcgg cccgcgacag gccgcgatgc gcgtacggct
     4801 attcgaactc gttgaagaaa acggcgagaa cgacggaaac ctccttcccc gaggtcttga
     4861 tgttctcctt ctccagcgcc tcgacgagct gctgcttgct taacttcgga ttgcccgcct
     4921 tgagctcgat cacgcgcttg atgacggaaa actgcaccgg cgcggggccg ctgaagaaac
     4981 ccgtattgtg cagcgtcatc accgcatagc ggacccgctc ctgcgtcttc tcgagctcct
     5041 cgatctttcc aataaggtat tccgggtcgc gcttcttatt gaagtacacc gggtccttga
     5101 ccgcctcggc gatcttcgcg cacaggcgat cctcggtcat gaactctccg cggatgcgca
     5161 ctgcgaccat cttctcccgg ttgttcttgt cgggggcggc gatgcggaag ttgtagcgca
     5221 ggtcgttccg gtaggtgaac agaagccccg aggtgatata gggcaggagg ttttggcccg
     5281 tgaattcgag ctgcgcgggc gtcaggttcg cgtccagcga gtcgcgccca acggcgaact
     5341 tcacgtcctt gatcttgacg gtcttgaaat tgtagaaatc gtccttgagc gtgaggatga
     5401 gcttgcccat gttgtcgctg atcttgtcgg agaagaccac ggtgaaggcg tttccccgcg
     5461 cgtccttgag gagaagctcg gtttcctcct cgccctcgtt cttctggacc acggtgatgc
     5521 cgctttgctt ctcgaactcg aaggccgcgt gcgcgttggc ggcgctcagt gcgagtatga
     5581 tggcgaggaa cagcagtttt ttcatcgcat tactcccctt gctgcttgat aaggatttta
     5641 tcgaacgggt ggaaggtgtt gatgccggcg agctccacgc gcttcgccct gatctccccg
     5701 cccgccatgt agaattcaat cgttccgata tactggtcgt cctcgcggaa gaccatcgcc
     5761 cggtcgccgg accggacctt gagcaccttg ctcacgtaga cgcggatact gtcgctgtcg
     5821 cgcgcgtcca cgatgaaccc gctctcccgg ttctgtcgcc cgtactgctc gagcgcgtac
     5881 tggtagccgc ggatcgcggc ctcctgccgt tgcgtgagcg agaagagcgc ggtaacgagc
     5941 gtctcgtagt cgttcgccga ggatttcatc agatggtgca tctgcgcgag cgacgggcgc
     6001 accgagttca cgtacgggat ctgcagcagg cggtccatga tgagcagctg attgtccacg
     6061 ccacgcttca gcttttcggc gccggcggcg gtgatgagct tcttggccgc gagctgctgg
     6121 tagagcttgg acgggtcgcc gcgcttgacc gcaggcgagg tgctcgcgtg gatgacggcg
     6181 ttgagccggg cattgtcgta cttcgggttg tacttgagga tgatctggtc gcgctgctcg
     6241 ttcatcttga gctcgtgcag gcgcttgtag ttgttcacca tctcttcggc cttcttcacc
     6301 gtctgctcga gcttgaagtc gtacgactgc atttcctttt cgagcgaggc gatctcgcgc
     6361 tccttggagc cgatgcggcc ctggtatttg ctcctgatct tgttgcgctc ggacgggctc
     6421 cccgcggcgc tcatctcctc gtccatcttc tttcgaagag attccagctc ggtctgggcg
     6481 ttcttgagct ggccctgaaa cttcttcgcg ccgctcaaga gctctcccag cttgaggtcg
     6541 tcgaactcgg tgatggagag gacggccttt tgctgcttcc attcggtgta cttggtgatc
     6601 cccaccgccg cgagcacaag gacggacatg aaggcgagta cgatgaacgg aaacagaagg
     6661 ctgcggttct ttttggtctt gctgaactca tcgtccagcg agtacacgtg ccgggccgcc
     6721 gagaggtcct ggatctcctc gggcaagaac gccgatttgc tctgtaccag gtcttttatt
     6781 tcgttttcat ttcccatacg ccgttccagt tcttcggtga tttgaacgtc ttggtccgtt
     6841 cgacgaactc ctccgccagc ggcagcgagc attttttgaa gaggaggtac gccttcttcc
     6901 agtcgccgtt atagtaggcc ttgaggcccg cggagtagtt ctcgaggttc ttttccattg
     6961 cgggggtgat ggtgctctcg atgagcggcc agtagacctt cttcgcggtg gtcttgccct
     7021 ttaccatcac ctgatcgagc tcgacgaagc ggatgccgtg gctgctcacg ttgtccacga
     7081 tgtccttgcg cacgtagtcg gagcagatca ccgggacctt gtagatgcgc gtgaggccct
     7141 cgagccggga ggcggcgttc acgttgtcgc cgatcacggt gttggtcatg cgctcgtagg
     7201 acccgatggt cccgtagaac acgttcccgc cgtcgattcc cacgcccacc tcgaggagca
     7261 ccgccttgaa cacgcggctc tccgaccggg agagcgcccc cttctccttc atgatctgct
     7321 ccttgcgctc gtgcatgcgc gcgcggagcg actcggcgac ctcctgcacc ttgtaggcgg
     7381 aggtgaccgc ctggagcgac ttgttctcgg tggattcctc gaacacgccg aagaccgcga
     7441 gcagcgcgtc gccgatgatc gagccgatca cgccgtcgag ccgcatgatc tcgcggatcg
     7501 cctggtcgta gtggaggttg aggagtttga tgatgtcggc ccccagggtc tcggtgatga
     7561 aggtgaagct cttgatgtcc gtgaacagga tggccagatc gcgctggttg ccctccagcc
     7621 gcacgtcacg ctccttatag gccttgatag cgatgtcgcg gctggtgaac ttgcggaata
     7681 tgttcaccag gttgttgatg gtgttcgaga gcgagttgaa cgcaaccccg aggtaggtga
     7741 tgtcgtcgtt gggcgccttc gacaggtcga tgagctcgag ctgctggttg gacaccatct
     7801 tcatgatccc cgaggtgatg atggggatgt aacgcaatat gaagcgcagc aggaagatcc
     7861 ccaccacggc gcttatgagc gtgacgagaa ggatgatgac gctgatgcgg atgaagatgt
     7921 tgcgcgactc ctcgtagaac tcgttgagct cctccgcgcg tacgatgtac acgctccagt
     7981 tcgcgttgtg cttgtaggcc ccgaagtagt cctcgttgtt gtagcggaag ttgatgaagc
     8041 cctcttgaat ggtgcggacc tggttgtcgg ccatttgctt gagcgcctgc gcgtcagcga
     8101 acttttcggc ctttttgatc ttggtcccct ggaagaggag cgtcccgtcg ggttttacgc
     8161 ccaagagcac cgacttcttc tttgtgaagt cccgcacgcc cttgttctcg atccccgcga
     8221 ccgcctcgtt gaggtccttc gtgtactgga agatctcgta ctggttgttg cagaaggtgt
     8281 tggcgtcctt gaggtccttg gtgagcaggt tccgcatgag gccgatgagc tccgtccggt
     8341 tgtagatgag gttgatgtag ttggacgaga gattggacac caggatgaac agggtgaaca
     8401 cgatgatgat cttgagcgtg accgggaaga cccgcgcctt gttgatcttt tgtccgaaga
     8461 ggttgtctag tagttttccc atgccagtgt atctttgtta ttcatccgtc cccgcgttgg
     8521 agcggaacga tacggggaaa gggatatttt tgtgatttcc aacttctgaa catgtactcg
     8581 ccgaaagtat tcgtgtcaat tcttttattg gcccgtttcg gaatctattc atccgttggg
     8641 tggtggaaat tttctttcct ttctctggtt agtatcgtat tattcatatg aaaactacgg
     8701 aatacttacg attcattcgt atgcgtacgg atcgtcgatg catgcgtgat acatggacac
     8761 aaggggttat tgaacatccc atccatgagg tagtccagcc tgatgggcgg atcagaagat
     8821 ggggcgttat agcggaagca cacggacggt atccgcgggt catagtgctt cctgacggtg
     8881 aaacggttca cgatgcattt ttcgacagat gtttaagggc ggcttgaaat gataatcaag
     8941 catttctctg atacagacac tctctacgtt cagtttagca acagggatag tattgagaca
     9001 cgggatctcg accggaatac cgttattgat ctggatgcca atggcgagct tgtcgcgatg
     9061 acgatcgagc atgccactga aacggcgtcg atgcacgagt tctcttttca gtccaagagc
     9121 caatctgcgt actagctcca gggaatcgat ccgatccgcc gccgccggaa ctgcccgacg
     9181 gcgccggatc gcgtcatcat gaaaacgccg tatcgacgtg gatggcctcc gccggcatgc
     9241 cccttccggc aagcgagcgg gtcgccgcgc caatcatggg cctcgcaccg cagaggtacg
     9301 cctcggcgtt ttcggtagta acggcgtcaa tgagatcggt gaccatgccg cgatgtccct
     9361 gccagccgtc gtcgggacgg gcgagggcga tcgcgtaatc gaagttgggg tgctcgcgcc
     9421 tgagctcctc gaggtactgc gcatagatta cgtcccgccc cgcgttcgcg ccatagagca
     9481 ggcgcaccgt tcgcccggga tgcttccggg ccgcctcgcg cgccagagaa aggaaggggg
     9541 tgatgccgat gccgcccgcg ataaagagct tgtcggtgtc ggtgtcgcgc acggtgaaat
     9601 cgcccatcgg gccctccagg cgcacggtat cgccttcccg catggcgaag aggcgcggcg
     9661 tgccccagcc gttcgggtcc agcttcacgg cgattttcac ccgggacgga tcgtccgttg
     9721 ggcgcgaaac gctgtagctg cggaacacct cgttctcgtc gtccacgcgc acggcgacga
     9781 actgccccgg tttgggcgtg agggtttttc catcgcgcaa ccggaaggtg aactcgcgga
     9841 tgtccgtgcc gatctcttcg acggactcga tgacggcgtc ggccgccgcg cgatccgcat
     9901 aaccgggaag cggctcgggg aggcgatagg tatcggtttt tctttcgctc tcgagggcga
     9961 ggacgttccc ggcgcagtgc gcgacgcaac tgccgcactt gatgcagtcg agcgcggcca
    10021 cctggtcgac gtcgttgagc tcgcggtagg gggcgagctg catggggcac acccgcgcgc
    10081 attttccgca cttctggcag gccgacggct gcgtcatggt gatgcgcgga ttcgcgaaca
    10141 ccggtccgat gagggcggtt cgcgccgcgt gcaggagctt ctgcacggtc cccatggggc
    10201 agaagctgca ccaggtgcgg ggattgacga ccagcgacag caccaggccg atgcccgtgg
    10261 tcgcggcgca catcatcacc cccaccatgc ccaggcggtc ccagaaggag accgttcccc
    10321 aggccgaaaa ggcgttcacg gtattgcgga tgaagaaccc catgaggagc gccaggacca
    10381 gcgcgcggaa ccacgcgctc gtgaacaggc gcgggatgcg gtcgaaggcg ccgatcgcgc
    10441 ggacggggaa gtcgaagagc gccccgcgcg ggcagaaatt gccgcaccag tagcgcccgg
    10501 tgaaaaagcc catgaccatg atggcggcga agagcggaaa gatgaacagg cccagcttcg
    10561 gctggaaaaa accggcgatc gatacgacgg gcacgatgag ccagataaaa cggcgcagcg
    10621 tggtgatggt gtcgaaggtt ttgatgtggt attgcgcgct cattgaaatc tcctgatgtc
    10681 tgattgttaa gtattaaagc aaatactaaa atacatggat ttttgtcaag cgggaatggg
    10741 aataaagcaa aaaatacgat gtctctgttg ttgcgcgaat aatggataca aaccactccg
    10801 ctatcttggc gtattttgtt tgccaataat ttgagctgac cactaatctg accaaaaaaa
    10861 agtatattca tttttggtct tgacgaaaaa taagtttatt gtattatggt cgccatgaat
    10921 aatgaacggt acctcttggg agcaatcaac gcagatcttg gcaagaaaat ggtcttcatc
    10981 ggcggacctc ggcaggtggg aaaaacgtat ctctccaaac gcctgttgga cctgaatgct
    11041 tcggatcagt acctcaactg ggattcgtcg gcccaccgga aaatcatttt ggggaaaacg
    11101 tgggaccgga cgaaagcgct agtggtcttt gatgagttgc acaagtatcg accgtggaag
    11161 tcgtggctca aaggcgtttt tgatacggag gagcgtccac cggctgtcct cgtgaccggc
    11221 agcgcgcgat tgaatgtctt caggcgcggc ggcgattcga tgatgggacg ctacttctac
    11281 tttcggttgc acccgttcag tcttcgggag cttgtcgaat attgcgattg gcccccggaa
    11341 acggccctgg ctgcgctctg ggagcgggga gggttccccg agccgttgtt ttcggaatcg
    11401 gcgctcgacg ccgcccggtg gaggaagtcg cgcctggaaa cgatcatccg cgaggacgcg
    11461 ctcacgctcg agaatgtgcg ccatctcgcc tcgctggagc tcctcgtgga cctccttcgc
    11521 gagcgtgtcg ggtcgcccat ctcctacaaa gcgctttcgg aagatctcgg ggtgagcccg
    11581 caaacggtga agtcctggat cgatctgctc gagcggctgt atctcgtgtt ttccgtgacg
    11641 tcgtatcatc gctcactggc acgagccctt aaaaaagaaa tcaaggtcta tttttacgat
    11701 tactcggaca tcggggacgg cggcgcgcgc tttgaaaacc tgatcgcgtc gcatctcatg
    11761 aaggaggcgc actatctcga ggacgtacag gggaggcgtg tctcgctgca caccctgaaa
    11821 gacaaggaaa agcgcgaggt cgatttcctc gtcgcagaga acgggcggcc gatcgagctc
    11881 gtcgaggcga agctcgggcg tgattcgttt tccgcaccac tcatgtatta tgcacggcgg
    11941 tttccgggta ttgcggcaac ccaggtcgtg aaggacctcc cggaatcgcg cagccgcgac
    12001 ggcgtgcgga tggtgggcgc gcgcgatttt ttaacgggcc ggggggtgtg aggccgtgcg
    12061 atttgcgact gttttgcatg ggatgatcga agcggcctgc tgaatcaagc gagaatttcc
    12121 tttgtgccgt cccggtttac cggcgcaccg aatgaggctt ttccggctgt atgtctttgc
    12181 atgccggatg aaacgatgaa acaatttatt acgcaattga aatccgcagt gataccgttc
    12241 cactcgtctc gatacttcga tttgttgatg gtctccgccg aaaacatgcg gtgaggtgac
    12301 cactccgatg gagatgatcg aggaccaatg ggcgatcagc ctcagcccgc cagaaatcac
    12361 caggcatggt atgcatcacg ctccatctcc cccatcgccg tatattgctt gcgacgcgag
    12421 gtttttcgct accactcctt tgcccggaag cccggcgcgt tgaggacggc gatggggctg
    12481 tcgccgaggc cgaggacgta aaagccgcag cgttctccgt atgcggtgag gccgggatcg
    12541 acgtacaggc ttgcgagcag gcccactagc ttgtggtctt tgtactcggg aaagaactcg
    12601 cggaattctc ccatctcaac gatgaaatcg tcgatgtagt ttttccgcat ggtccccttc
    12661 gtcgaattga cgaacacgta ctcgccggcc accgcgatgg cgtcaaactc gcggttccgg
    12721 ccgcccggga gcgttttttt cctcctgata ctcaggtcga ccgtctcgag gccgtaggta
    12781 tccttaatga cgcgcgggag gccggggaag atgatgtcct cgaccagcgt gcccatcttg
    12841 ttcgagagct cgccccactt tttgttcatc tccttgattt gacgggcgcc atcgtccctg
    12901 aagtccttca tttcggcctt gaattcgcgc atttcctgtt ggaatgcctc aaacgatatt
    12961 tgattttgcg taaccatatt gtacgcttta ttgaactcaa ttccgacatt acggatgaaa
    13021 tcgtccaatg tttccctcag cgtgtccacc ttcttctcca actcgatcac ggttctttct
    13081 ccaaggaatt aagtttagta taatccggta atcaccggaa aacaagcgat ttttgcacgt
    13141 tcgccagctc gttggttgtg tcaatatgag atggtgagcg cgtatgccta ttgtttatac
    13201 gatggggatg ctggttcatt tgatcatttt aatgaaaaaa gtcggcattt tttgcattat
    13261 gatgatgggg caggatatga cccttctgcc ggtcccgcat ggtcatgttt gacaggctgt
    13321 gtaaaatacg tccaagcggg taaaatctga aaatattttg tgtcgattgc gcccgttttc
    13381 gcgcttatgc gcggctgcct gtggatacgg gtgccgaccc ggtttctcgg gtaattagta
    13441 atcaatcgga ggggggtttt tatgaacact atttatggtc gtagagtagg tgagacgctt
    13501 gttggattat tgtcctgtat ggtcatggca tttgcttcct gtagcggcag cggtggtggt
    13561 gatggcggcg gaggtggcgg gggcggagct gctggaagcg ctggtgtgag tacactagct
    13621 gtaaagggca gccttggtgc cgcttttgcg atgaacaagc caatgaaatg gtatgatcga
    13681 ataatcgcac tagtatcttt ttctggtaat ttatatgcgg gtcccgatag tgtggataaa
    13741 attgtcgctg ttccagtgga tcactcgttt tcgagtaatg aaaatctgat tgagagcgca
    13801 ataactatcg atattaatcc cgataaaagc tttaccatca atctagaccg aactaagcgg
    13861 tgggtccttc ttttagtgaa ttctaccgca tcatggaaaa acagagtagt ggcatttgcc
    13921 gcccttggcg atactaataa ccttgtattg ctaccgctgg ataatgcaac atcaaatatt
    13981 gaactcggaa atctttcaaa atcagggagc gacgctgttg aatccggttc gctaataggt
    14041 aatacagcat catttagtac aaatatttca aagcttcgcg aagttgccca gatgagctgc
    14101 atgacaaaag tgataaaaaa catgtatcaa aatcatgtcc gcaagctcga aagtggtggt
    14161 gaatattact attcttggac tgatcataat tggatggtga attctgcggg ggccgagaat
    14221 agcttcacga atccatcttt gtacacctac aaggggtatc atccaacttt tagaacagaa
    14281 aaatatagcg aaatatcatt ttccggaatt tatgataaag attacacggt tagcataaca
    14341 gctcctgctg ccgtttcacc ttatggcggt ggggggccct ttactgtttt tgacaatacg
    14401 aaggcatggg acgggtcaag aacgcgacct ggtgaatatg cagtcactct tgatgctaca
    14461 ccgccaaagt ttgcgattat gtatttctcg cgtaactatt cagcactttg aggcataaat
    14521 ggcctgtttt caaacaaccc ctgta
//
LOCUS       DAORHF010000079        14452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3246_length_14452_cov_5.956380, whole genome shotgun sequence.
ACCESSION   DAORHF010000079 DAORHF010000000
VERSION     DAORHF010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14452
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3246_length_14452_cov_5.956380"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PKJ16_08360"
     CDS             complement(<1..656)
                     /locus_tag="PKJ16_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNV47039.1"
                     /translation="MESSLFYLAPIGSVIALLFAIVFYKLMMGANEGNAKMIEIAGAV
                     REGAMAYLTQQYKVVGFVFFCLVILLSILAYLGIQNPFVPFAFLTGGFFSGLCGYLGM
                     KTATNASSRTAQGASESLNRGLQVAFRSGAVMGLVVVGFGLLDISIWYVVLNYLYDNN
                     VANLSAGLVEKFHIAGGVFSTAVLSSGEFQHAKLIEVTTTMITFGMGASTQALFARVG
                     G"
     gene            complement(945..1484)
                     /locus_tag="PKJ16_08365"
     CDS             complement(945..1484)
                     /locus_tag="PKJ16_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47040.1"
                     /translation="MMDRAKNSVAALWLALAMLAGCRPDGGVAVTALAVGSRLAPDGS
                     IENADTVYRFPRARMRSARTMRALADRVYFAGDTVCFSAEFTRPIARGGARVWFVDPA
                     GGARHAAERIEYDGARVHGFSLVGSLMREFAGTGIGRPLPEGVLRHEVPFAVIVTLPD
                     ADPAAERRIDGRILVEIVD"
     gene            1641..1913
                     /locus_tag="PKJ16_08370"
     CDS             1641..1913
                     /locus_tag="PKJ16_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HNV47041.1"
                     /translation="MADTNKKTVLRRLMTLPGVGASIADDLYGIGVRAPEELRGADPE
                     ALYERICARQKARIDRCLLYVMRCAVHYASTETRDPELLKWWNWKD"
     gene            2057..2716
                     /locus_tag="PKJ16_08375"
     CDS             2057..2716
                     /locus_tag="PKJ16_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HNV47042.1"
                     /translation="MDANTTLTRLRSLADPANRAGMARFGIDTERALGIKIPELRRLA
                     KEIGTDHDLARALWDTGVHEARILAGLIADPSRATLPLLDRWARDFNSWDLCDLVCAN
                     FIDRTRVAWTLPARWARREEEFVRRAAFALIAALAVHDTAAPDERFVRLFPLIKRYAT
                     DERNFVKKAVNWALRQIGKRNARLRKRAIALAREIRAIDSRSARWIAADALRELNTRK
                     K"
     gene            2725..3360
                     /locus_tag="PKJ16_08380"
     CDS             2725..3360
                     /locus_tag="PKJ16_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV47043.1"
                     /translation="MSEIFYSIEGETTRAGFPAVFVRLAGCNLRCRYCDTRHAWEGGA
                     AMGIDEIVARVDRYASADHVTVTGGEPLLQGECGALARRLLAAGRAVQIETNGSVSLE
                     AVPAGARSIVDVKTPSSGEAGSFLTDNVRRLRAGDELKFVISSIEDYAFSREFIAANA
                     VPAGVAVNFSANVDEMSFTKLAGMILDDRLPVRLNMQLHTIIWPRGEPVHR"
     gene            3370..4242
                     /locus_tag="PKJ16_08385"
     CDS             3370..4242
                     /locus_tag="PKJ16_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV47044.1"
                     /translation="MDYVGNVIRPPSEAYSMIIQVTVGCSHNQCNFCGTYKGYGFYVK
                     DIDQVKRDIDEAARYKFTRAFLADGDVLILPTEKLLDIITYLRERNPRVERIGVYGNT
                     KAILKKTVEDLKLLRDAGLGIIYHGIESGNKEVLKRIKKGAYPHKQLEAAEKVKAAGI
                     QLSQTVLLGIGGTELSEAHAIDTGKHLGAMSPDFASALTVMLLPNTGLYRDFKAGSFR
                     LPDKFGLLRELRLIIEHMDVTRECFFTSNHASNYLPIRATLPGDRAAVLRLLDQVLGS
                     RDESVLKPEHMRAL"
     gene            4253..4417
                     /locus_tag="PKJ16_08390"
     CDS             4253..4417
                     /locus_tag="PKJ16_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47045.1"
                     /translation="MESSSLKEAVKYISGELKADPAIDKNALIDEASRRFDLNPMQTE
                     FLINKYILGA"
     gene            4434..6929
                     /locus_tag="PKJ16_08395"
     CDS             4434..6929
                     /locus_tag="PKJ16_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV47046.1"
                     /translation="MPAPGHAAAIGTFIVPIVCCTLLCAGAALFGYFHARSRERGYLA
                     IMLVGLLGACYTAAEALILVTSADGGPAGAPLQYYRIKQIASAFYLFGLPFLLQDYLD
                     LGDAWKRLNLRLALGGLAAAVALSVIAVIIPDHFVSVTAADPLSIAHPVPHVRGLPGA
                     AYHARTALLGAMILYAIAAIAFDMRRNRRLGTPLAVLIGMLVAIATGAVDIVHVYRPI
                     TGIALIDANFSRFTVGITTLTVLMMTVSLHDAASRALEATSARLNMEGAIKTIDKSER
                     RFLQLAENISEIFIIHDCANHVVLYVSPSYETVWGASCMSLLSSPDSWLAAVHPDDRT
                     RVMDAFDALPLPDRFELEYRIVAASGVTRWIRDRVSAVRNERGETYRLVRVLDDITER
                     KENEERLIHLAYHDMLTGLPNRKAFFERIDDTLVQAKRLNGQHQKALLFIDMENLKEV
                     NDTLGHDVGDEALVKATARLRSVLRESDYIFRFGGDEFTVILSFITDGLDAGIVADKI
                     IRSLSQAYRIHDSEIFLGLNVGISLYPKDGDAPETLIKNADIALNESKRERNAYKFFE
                     AEMTARAQDRLEIKNRLRHAIDGSQFAMHYQPFVDAAGRIVGMEALIRWDAPGRGLVP
                     PDVFIPIAESTGLIVPIAQWTLHTACAQMRRWTDAGHAGLRVAVNLSPRQFMEDNLAD
                     TVTRILRDTGCAPERLELEITEGSMMEHPEAAVEKINELRRLGVTFSIDDFGTGYSSL
                     SYLTRFRINNLKIDKSFILNITRESEYQEITRAIIAMAHNLKIAVIAEGVETAEHASF
                     LRALGCDAMQGYHFSRPVPPEEFARLLEKKTLA"
     gene            complement(6936..7883)
                     /locus_tag="PKJ16_08400"
     CDS             complement(6936..7883)
                     /locus_tag="PKJ16_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47047.1"
                     /translation="MTILHKYILFQSLIVTAFAIGFLLRKRHANPERLSSRLVMANLV
                     LIEPPIALWSIWGLALGADLIVLPAAGVALVLAGFAFGVPLARLLGLSGRRRATFLIS
                     ASLANHGFTMGGIVCYLLMGERGLGLSFIFVSYFSFYLYAVVFPYAEFHTGASRPLGE
                     RLRELVFNARNMPLYAACAGMALSAAGIARPAAAVPIDALLMASVAAYYLALGLNFAL
                     DDVRVSMGAHVALGALKFALVPLCAIVPLAFLEVDPAVKSVVIVQSFAPAAVYSVVTA
                     VVFRLDARLASGMFVVNTLVYLGAVLPAIFLFGRALLPR"
     gene            8236..9792
                     /locus_tag="PKJ16_08405"
     CDS             8236..9792
                     /locus_tag="PKJ16_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV47048.1"
                     /translation="MKPFRVILIAPPIHDFYATPARMEPLGLLYIAAALRRMPGVRCD
                     VYDAAYSMKKKRIRTPECFGHLRDIYTEDTSHFSLLSHFFRFGDSFDRIVAAVREGAY
                     DLVGVSSMFSGYHPDVEAPAARIKAETKSPVAIGGWAVFADADRLAGATAADFLISGD
                     GETAFPELVRRLMDTETAAGGADLPRRGERTGGTVRIHGGGVDDSPLDDFPEREAVYR
                     FRGRRIARMVLSRGCRHSCAFCAVPRRYAFTARSIESISRELEYLHGIGVGIVDFEDD
                     NLFHDQAFSRELMTLLREHHAKGMSFAAMNGITAPNLVPFVNEAIDAGFIEFNVSLVT
                     GSASAARGVNRPSFREAVRVIAEKSAGRIPMLAFIIGGLPGTTPAGVIDDIVFLAGLP
                     LTIGFSPLYLLPGLPVFEALGLPDDARLLRGSALWKFGEGFAREDVASLWKYVRMINR
                     LKATCAAPTDDERDDLRYFSRSLREKTWYAKRRDGRWERTFRFSIDLPMSLRVTHADG
                     ESALWDFDGV"
     gene            10160..12130
                     /locus_tag="PKJ16_08410"
     CDS             10160..12130
                     /locus_tag="PKJ16_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNV47049.1"
                     /translation="MNIIAIISSTILLLTTALPAAAFTARELFVDTGCHTARVWNIAR
                     SNDGTQIVTSSQDKTLRVCDAKTLQPVRIIRGQTGQHGWGMEYGAKLFLNDTYLVTRG
                     LYGTPYGAGHDIRVYDFKTGRSYCSLFSDDGMDKAFAISEDGEHLIAGNTKGEIYVFG
                     MKEILDRSQKHPVLRIDRVNGAKLKDDYITVLHKYRSDPAKIDLKPAYQPDRTSLVEL
                     SSHTKDGDVRILSISKECVSLYSLTRRTMLKSSPLKDVKKARITRHYAIASDSDGITI
                     YDAELNRKATFTMAHKVHDLRISPDERTLAIMLANRECALYDIRSGAIRKLRSDGRLD
                     VSAMEFIDNKTLAFADENAFTLFAVDTATGKELPRSAGLEALPIWGLGARGSEIALGT
                     EPYVAPGDQPELEIAFDLKTFTARPAAGGAPFAPLPLEQGVFRAKWKRGEKRDSIELY
                     RGNTLVSAITPDERTHPWLWTYGITPYGFVVAGGPGGTLLQWLTVTYDDGNPVKGACT
                     DHAAAVTSFACSDRHLFSGSIDRTIVIYDLHKMLDAFRAELVKGKTREDSTASDPKGK
                     KEADVFTIKSTYSLRKLISPEVLARVMIGRNGEYCIWTPDGYFAASEGFRGRVGIFDY
                     KGEGREATWRSGKEYFDDHHRPDIIRKRLEFE"
     gene            12207..12689
                     /locus_tag="PKJ16_08415"
     CDS             12207..12689
                     /locus_tag="PKJ16_08415"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV47050.1"
                     /translation="MTTTGFQDHYPDDYAHCYGCGRLNEHGMRLKSVWDGPESVCRFT
                     PRPHQTGGLRDIVYGGLIASLVDCHCAGTAAAAKLREEGFQPGERPMPRFVTASLKVD
                     YIAPTPIGVEIEVRARVVEMKGRKAVIAATVSAGGTMTAKGEAVMVQLRETASATEER
                     "
     gene            12836..13561
                     /locus_tag="PKJ16_08420"
     CDS             12836..13561
                     /locus_tag="PKJ16_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV47051.1"
                     /translation="MNIIAISNLSKRYGRSIQALADFSLAVERGTIMGLIGPNGAGKT
                     TLIKSILGLAHADAGEIAINGITGADPAARASIGFLQEKFNFFPTYTARNALAFFAAL
                     KKPAGVAHVDIAGMIDTVARTVMITELLDRKIKTFSKGELQKVGIAQALLYEPDLLIL
                     DEPFSGLDPIITKELKDIILAYRAKGKTVFFSSHILGDVAQLCDRVAIINKGVLVREC
                     AVGDLTGDLTLEDAFYNIITGKN"
     gene            13568..14404
                     /locus_tag="PKJ16_08425"
     CDS             13568..14404
                     /locus_tag="PKJ16_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47052.1"
                     /translation="MNQILVLVQNTIKKEFTNKLLIFMFAVTVLIIYVLGNISDFVVK
                     NISMTGPGGQQSASAVSAAAGGYSFAAFFFLIQSWNALLAVLVTIGGIGSDYRYGVIT
                     QILSQPLPRWKYLVSRIAGSYLIILAYYVISLALAFVFLSGNFSAQSLTPTLAGSFAV
                     AQLVILFYITVSVIMALYVPQKIAFFLVFILALFLHYANYHAGALLASGESVLAKPVL
                     VLATFAFPQIGTISKFSSFSFTPDASYSAAEVLGHSALFLAMLAAWFVGACFAYRRKD
                     IS"
BASE COUNT         2809 a         4772 c         4458 g         2413 t
ORIGIN      
        1 ccgccgaccc gggcgaacag cgcctgggtg gatgcgccca taccgaaggt gatcatggtc
       61 gtggtgacct cgatgagctt cgcgtgctgg aattcacccg aggaaaggac cgccgtgctg
      121 aacacgccgc cggcgatgtg gaacttctcg accagtccgg cgctcaggtt ggcgacgtta
      181 ttgtcgtaca ggtagttgag gacaacgtac cagatcgaga tgtcgaggag gccgaagccg
      241 accacgacca ggcccatgac ggcgccggag cggaacgcca cctggaggcc gcgattgagc
      301 gactcggagg cgccctgcgc ggtgcgcgag gaggcgttgg tcgccgtctt catccccaga
      361 taaccgcaca ggccggagaa gaaaccgccc gtcaggaagg caaagggaac gaagggattc
      421 tggatgccca gatacgcgag aatggataac aggataacga gacagaaaaa aacaaacccg
      481 acgaccttgt actgctgggt gaggtacgcc atcgcgccct cacgaacagc gccagcgatc
      541 tcgatcatct tcgcgttacc ttcgtttgcg cccatcatca gcttatagaa gacgatggcg
      601 aaaagaagcg cgatgaccga tccaatgggc gcaaggtaaa acagactgct ttccatatgg
      661 actactcctt ttactttaaa atcgtacggc agatcacaac gatctgtgcc tgtgccgcag
      721 gctgcacgga tcccgcttgt ccgagccgga ggaaaccgtg caaataaaat tttttattca
      781 aattccatag aaaaaaaatg gcccgtattg tcaatcgaat tttggcagct tcgattccga
      841 tcgcggtgaa aagggccccc gggcgccgat tcggcaggct ttcaccgcgt ccacaagcaa
      901 gaagggcatt atccgccagc tatgatccct cagccgcttt gtcctcagtc aacgatctcg
      961 accaggatgc gcccgtcgat acgacgctcc gccgcgggat cggcatcggg cagcgtcacg
     1021 atcaccgcga acgggacctc atggcgcagc acgccttcgg ggagcggccg gccgatcccg
     1081 gtcccggcga attcccgcat gaggctcccc acgagcgaaa atccgtgcac ccgcgcgccg
     1141 tcatactcga tccgctcggc cgcatgccgc gcgccgccgg ccggatcgac gaaccacacg
     1201 cgcgcgccgc cccgcgcgat cggtcgcgtg aattccgcgg agaagcagac ggtgtcgccc
     1261 gcgaaataga cgcgatcggc gagggcgcgc atcgtccggg ccgagcgcat ccgcgcgcgc
     1321 gggaagcgat agaccgtgtc cgcgttctcg atggagccgt cgggggcgag ccgcgagccc
     1381 acggcaaggg cggtgacggc gacgccgccg tccggacggc acccggcgag catcgcgagc
     1441 gcgagccaga gcgcggcaac cgaattcttc gcgcgatcca tcatcacccc cgcaccgccg
     1501 tagcgccgtt tttcgcgaca ccgaagcgcc gatatcgcat cgatcacaga taccggcgcg
     1561 cgcggcgtcc tgtaaagtga aaaccaaaaa gtgattgccg ccggaggacg ggacggccag
     1621 gctaccggag gagtcgcgag atggccgaca cgaataaaaa aaccgtcctg aggcgcctga
     1681 tgacgctgcc cggcgtgggg gcgtccatcg ccgacgacct gtacggcatc ggcgtgcgcg
     1741 cgccggaaga gctgcgcggg gccgatccgg aggcgctcta cgagcggatc tgcgcgcgcc
     1801 agaaggcgcg gatcgaccgc tgcctgctct atgtgatgcg ctgcgccgtg cattacgcct
     1861 ccaccgagac gcgcgatccg gaacttctga agtggtggaa ctggaaggac tgacctcacc
     1921 ccccggcccc gcttcgacag gctcagcgca tcgcctcctc tggagagggt gtaactgaga
     1981 aaattgttcc aggccaagcc cctctcctgc gggagagggg cggcagagcc ggggtgaggt
     2041 acatgatcga aataatatgg atgcaaatac aacactaaca cggctccgct ccctcgccga
     2101 ccccgcgaac cgtgcgggca tggcgcgctt cggcatcgac acggaacgcg cgctgggaat
     2161 taaaatcccc gagctgcggc ggctggccaa ggagatcggc acggaccacg atctcgcccg
     2221 agcgctctgg gacacgggcg tccacgaggc gcgcattctc gcgggcctta tcgccgatcc
     2281 gtcgcgcgcg acgctgccgc ttctcgatcg atgggcgcgc gatttcaact cgtgggatct
     2341 gtgcgacctg gtgtgcgcga acttcatcga ccggacgcgc gtcgcgtgga cgctgcccgc
     2401 ccgctgggcc cgacgcgagg aggagttcgt acgccgcgcg gccttcgcgc tcatcgccgc
     2461 gctcgccgtg cacgacacgg ccgcgccgga cgagcggttc gtgcgccttt tcccgctcat
     2521 caaacgctac gcgaccgacg agcggaactt cgtgaagaag gcggtcaact gggcgctgcg
     2581 gcagatcggc aagcggaacg cgcgcctgcg gaagcgggcg atcgcgctcg cgcgggagat
     2641 ccgtgcgatc gactcgagat cggcccgctg gatcgccgcc gacgcgctgc gcgagctgaa
     2701 tacacgaaag aaatgacact aaccgtgagc gaaatcttct actccatcga gggcgagacc
     2761 acgcgcgccg gatttccggc ggtgttcgtg cggctcgcgg gatgcaatct ccgctgccgc
     2821 tactgcgata cgcggcatgc ctgggagggc ggcgcggcga tgggcatcga cgagatcgtc
     2881 gcccgcgtcg atcgctatgc gtcggcggac cacgtgaccg tcaccggcgg cgagccgctc
     2941 ctgcaggggg aatgcggcgc gctcgcgcgg cgcctcctcg cggccgggcg cgccgtccag
     3001 atcgaaacca acggcagcgt gtcgctcgag gccgttcccg cgggcgcgcg ctcgatcgtg
     3061 gacgtgaaga cgccctcgag cggcgaagcc ggatcgttcc tgacggacaa tgtgcggcgc
     3121 ctgcgagcgg gcgacgaatt aaaatttgtc atatcctcga tcgaggacta tgctttctcg
     3181 cgggagttca tcgccgccaa tgccgttccc gcgggcgtgg cggtcaactt ttcggccaac
     3241 gtcgatgaaa tgtcatttac aaaattggcc gggatgatac tcgatgatcg gctcccggtg
     3301 cgcttgaaca tgcagctgca cacgatcatc tggccgcgcg gcgagccagt acacaggtag
     3361 gaaacgagca tggactatgt cggaaacgtg atacggccgc cctccgaggc ctacagcatg
     3421 atcatccagg tgaccgtggg ctgttcgcac aaccagtgca acttctgcgg cacctacaag
     3481 ggatacggtt tctacgtcaa ggacatcgac caggtgaagc gcgacatcga cgaagcggcg
     3541 cggtacaaat tcacccgcgc cttcctggcc gacggcgacg tgctcatcct gcccacggag
     3601 aagctgctgg acatcatcac ctacctgcgc gagcgcaacc cgcgcgtgga gcgcatcggc
     3661 gtatacggca acaccaaggc gatcctcaag aagaccgtcg aggacctgaa gctgttgcgc
     3721 gacgcggggc tgggcatcat ctatcacggc atcgagagcg gcaacaagga ggtgctcaag
     3781 cgcatcaaga agggcgccta cccgcacaag cagctcgagg cggccgagaa ggtgaaggcg
     3841 gccgggatcc agctctcgca aacggtgctg ctggggatcg gcgggacgga gctctcggag
     3901 gcgcacgcga tcgacaccgg aaagcatttg ggcgcgatgt cgcccgactt cgcctccgcg
     3961 ctcaccgtga tgctcctgcc caacaccggc ctctaccgcg acttcaaggc gggatcgttc
     4021 cggctgcccg acaagttcgg cctgctccgc gagctccggc tcatcatcga gcacatggac
     4081 gtgacgcgcg aatgcttttt cacctcgaac cacgcatcca actacctgcc catacgggcg
     4141 acgctccccg gggaccgggc cgccgttctg cgcctgctgg accaggtgct cggctcgcgc
     4201 gacgagagcg tactcaagcc cgaacacatg cgggcgctgt aaggagggtc tcatggaatc
     4261 atcctcgctc aaggaagcgg tcaagtacat cagcggcgag ctgaaggccg accccgccat
     4321 cgacaagaac gcgctcatcg acgaggcgag ccgccgcttc gacctgaacc ccatgcagac
     4381 ggagttcctc atcaacaagt acatcctggg cgcctgaccg gccggtgccg ccaatgcccg
     4441 cccccggcca cgccgccgcg atcggcacct tcatcgtccc gatcgtctgt tgcacattgc
     4501 tgtgcgcggg ggccgcgctg ttcgggtatt tccatgcgcg atcccgcgag cgcggctacc
     4561 tcgcgatcat gctcgtggga ctgctgggcg cctgctacac cgcggcggag gcgctcattc
     4621 tcgtcacctc cgccgacggc ggtcccgccg gcgcccccct gcagtattac cggatcaagc
     4681 aaatcgcgag cgccttctat ctcttcggcc tccccttttt gctgcaggac tatctcgatc
     4741 tgggcgacgc ctggaaacga ttgaacctgc gattggcgct cggcgggctc gcggcggccg
     4801 tcgccctgtc cgtgatcgcc gtcatcatcc ccgatcattt cgtgtcggtt accgccgccg
     4861 acccgctctc gatcgcacac cccgttcccc acgttcgcgg cctccccggc gccgcatacc
     4921 acgcgcgcac ggcgctgttg ggcgcaatga tcctctacgc gatcgcggcc atcgcattcg
     4981 acatgcggcg caaccggcgg ctgggcacgc ccctcgcggt cctgatcggc atgctcgtcg
     5041 cgatcgccac cggcgccgtc gacatcgtcc acgtgtaccg gcccatcacg gggatcgcgc
     5101 tcatcgacgc gaacttctcc cgcttcaccg tggggatcac gaccctcacc gtcctgatga
     5161 tgacggtgtc gctgcacgac gccgcgagcc gggcgctgga ggcgacatcg gcgcgcctca
     5221 acatggaggg cgccatcaag acgatcgaca agagcgagcg gcgcttcctg cagctggcgg
     5281 agaacatcag cgagatcttc atcatccacg actgcgccaa ccatgtcgtg ctgtacgtga
     5341 gcccgtcgta cgaaaccgtc tggggcgcat cgtgcatgtc cttgctctcg tcccccgact
     5401 cgtggctggc ggccgtgcac cccgacgacc gtacgcgggt gatggacgcc ttcgacgccc
     5461 tgccgctccc ggaccgcttc gaactcgaat accgcatcgt cgcggcctcg ggcgtgacgc
     5521 gctggatccg cgaccgcgtg agcgcggtcc gcaacgagcg cggcgagacc taccggctcg
     5581 tgcgcgtgct ggacgacatc accgagcgca aggaaaacga ggagcgcctg atccacctgg
     5641 cctatcacga catgctcacg ggcctcccca accgcaaggc cttcttcgag cggatcgacg
     5701 atacgctcgt gcaggcgaag cggctgaacg gccagcacca gaaggcgctc ctgttcatcg
     5761 acatggagaa cctcaaggag gtgaacgaca cgctggggca cgacgtgggc gacgaggcgc
     5821 tggtcaaggc gacggcgcgc ctgcggtcgg tcctgcgcga gagcgattac atcttccgct
     5881 tcggcggcga cgagttcacg gtgatcttga gcttcatcac cgacggcctg gacgccggca
     5941 tcgtagccga caagatcatc cgctcgctct cccaggccta ccgcatccac gacagcgaga
     6001 ttttcctggg gctcaacgtg ggcatcagcc tctaccccaa ggacggcgac gcccccgaga
     6061 cccttatcaa gaacgcggac atcgccctca acgaatcgaa gcgggagcgc aatgcgtaca
     6121 agtttttcga ggcggagatg acggcgcgcg cccaggaccg gctggagatc aagaaccggc
     6181 tgcgccacgc gatcgacggg agccagttcg cgatgcacta ccagcccttc gtggacgccg
     6241 ccgggcgcat cgtgggcatg gaggcgctga tccgctggga cgcgccgggg cgcggcctcg
     6301 tgccgccgga cgtgttcatc cccatcgccg agtcaacggg gctcatcgtg cccatcgcgc
     6361 agtggacgct gcacaccgcg tgcgcgcaga tgcgccgatg gaccgacgcc gggcacgcgg
     6421 gcctgcgcgt ggcggtgaac ctctcgccgc gccagttcat ggaggacaac ctcgccgaca
     6481 ccgtgacgcg catcctgcgc gacacgggct gcgcgccgga gcgcctggag ctcgagataa
     6541 ccgagggcag catgatggag caccccgagg ccgccgtgga gaagatcaac gaactccggc
     6601 ggctgggcgt gaccttctcg atcgacgatt tcgggaccgg ctactccagc ctgagctacc
     6661 tcacgcgatt ccgcatcaac aatctcaaga tcgacaagtc gttcatcctg aacatcacgc
     6721 gcgagagcga ataccaggag atcacgcgcg ccatcatcgc gatggcgcac aatctgaaga
     6781 tcgccgtgat cgccgagggc gtggagaccg ccgagcacgc gtcgtttctg cgcgcgctgg
     6841 gctgcgatgc gatgcaggga taccacttca gccgcccggt gccgccggag gagttcgcgc
     6901 ggctcctcga gaaaaagacc ctcgcctgac cgcccctagc gcggcaggag cgccctcccg
     6961 aacaggaaga tcgccggcag caccgcgccc agatagacca gcgtgttcac cacgaacatc
     7021 cccgaggcca ggcgcgcgtc gagccggaag acgacggcgg tcacgaccga gtacaccgcc
     7081 gccggcgcga aggactggac gatgacgacg gacttcacgg cgggatcgac ctcgaggaac
     7141 gcgagcggta cgatggcgca taacggcacg agcgcgaact tgagcgcgcc cagcgcgaca
     7201 tgcgccccca tgctcacgcg cacgtcgtcc agggcgaagt tgagccccag ggcgagatag
     7261 tacgcggcca cggacgccat gagcagcgcg tcgatgggaa cggcggccgc cggccgcgcg
     7321 ataccggcgg cgctgagcgc catcccggcg cacgcggcgt aaagcggcat gttgcgcgcg
     7381 ttgaaaacca gctcccggag acgctcgccg agcgggcgcg acgcgccggt gtgaaactcg
     7441 gcgtagggaa agacgaccgc atacaggtaa aaggaaaagt acgatacgaa gatgaacgac
     7501 agccccaggc cgcgctcgcc catgagcagg tagcagacga tgccgcccat ggtgaagccg
     7561 tggttcgcga gcgaagcgct gatgaggaag gtcgcgcgtc gccgcccaga aagccccagg
     7621 agccgcgcca ggggcacgcc gaacgcgaag cccgccagga cgagggcgac ccccgccgcc
     7681 ggcagaacga tgaggtccgc gccgagcgca agcccccaga tgctccagag cgcgatgggc
     7741 ggctcgatga ggacgaggtt cgccatcacc aggcgcgacg agaggcgctc cgggtttgca
     7801 tgacgcttcc ggagcaggaa gccgatcgcg aaggcggtca cgatcagcga ttgaaagagg
     7861 atgtatttgt ggagtatggt catgtggatt tcacatccga gttattccgg aaatcgggtt
     7921 tcctgcttgt ccgttacttt cttttgggcg gccaaaagaa agtaaccaaa taaaaaccgc
     7981 tcgctcaacc tcaccccgcc tccggcgcgc cctctccccc aggagagggc tctgaattat
     8041 taaactccat catctcccct tctcctacgg gagaaggggt cgggggatga ggtggccccc
     8101 ttcacaagca aatgcgctct acctggaaag taattgcaca gaaaccgtgt accacactac
     8161 cctatcacca gcgtgcaacc aatcaacgtg cgcatttaaa aatcaacttt ttcttgagca
     8221 tatagcacct catcgatgaa accgtttcgc gtcattttga tcgcgccgcc catacacgac
     8281 ttttacgcga cgcccgcgcg catggagccg ttggggctgc tgtacatcgc ggcggcgctt
     8341 cggcgcatgc ccggcgtgcg ctgcgacgtc tacgatgcgg catactcgat gaagaagaag
     8401 cgcatccgga cgccggagtg cttcgggcac cttcgcgaca tctacaccga ggacacatcg
     8461 catttctcgc tgctctcgca tttcttccgg ttcggcgatt cattcgaccg gatcgttgcg
     8521 gcggtgcgcg agggcgccta cgatcttgtg ggcgtctcgt cgatgttctc ggggtaccac
     8581 ccggacgtgg aggcgcccgc cgcgcgcatc aaggctgaaa cgaagtcgcc ggttgcgatc
     8641 ggcggctggg ccgttttcgc cgatgcggac cggctcgcgg gcgcaaccgc cgccgacttc
     8701 ctgatttccg gcgacggcga gaccgcgttc ccggagctcg tccggcggct catggacacc
     8761 gagacggccg cgggcggcgc ggacctgccg aggcggggcg aacggaccgg cggcacagtc
     8821 cgtattcacg gcggcggtgt cgatgattct ccgctcgacg atttccccga acgcgaagcg
     8881 gtctaccgct tccgcggcag gcgcatcgcg cgcatggtgc tcagccgcgg ctgccgccat
     8941 tcctgcgcct tctgcgccgt cccccggcgg tacgccttca ccgcgcgctc catcgaatcg
     9001 atctcgcgcg agctcgagta tctgcacggc atcggcgtcg ggatcgtcga tttcgaggac
     9061 gacaatcttt tccatgatca ggcattctcg cgggagctca tgacgctcct tcgagaacac
     9121 catgcgaagg gaatgtcctt cgcggcgatg aacggcatca cggcgccgaa ccttgtgccg
     9181 ttcgtgaacg aggcgataga cgccggtttc atcgagttca acgtgtcgct tgtgaccgga
     9241 agcgcctcag cggcgcgcgg cgtgaaccgc ccgtcgttcc gagaggccgt tcgcgtgatt
     9301 gccgaaaaaa gcgcggggag gattccgatg ctcgccttca tcatcggcgg cctgccggga
     9361 acgacgcccg cgggcgtcat cgacgacatc gtcttcctcg cgggcctgcc gcttaccatc
     9421 ggcttctcgc ccctgtacct gctccccggc ctccccgtct tcgaggcgtt gggattgccg
     9481 gatgatgcgc gcctgttgcg cgggagcgcg ctgtggaaat tcggcgaggg cttcgcgcgg
     9541 gaggacgtcg cgtcgctgtg gaagtacgtc cgcatgatca atcgcctcaa ggcgacatgc
     9601 gccgcgccca cggacgatga gcgcgacgac cttcgctact tttcgcgttc gctgcgagag
     9661 aagacctggt atgcgaagcg gcgcgacggc agatgggagc ggaccttccg cttctcgatc
     9721 gacctgccga tgtcgctgcg cgtgacacac gccgatggcg aatcggccct gtgggatttt
     9781 gacggagttt gatacaccga cccgatttct aaaaaagatc caagctgatt ggcttttccg
     9841 gtataatctt aactttttgt tgtctttttc gctttgacga tgaaggcact ttcgccccag
     9901 gccgcatgta cacattcgta tcgaaccaaa gcgaactcaa agcgaactca agacgaactc
     9961 aaggcgaccg catagaggcc ttaaattctg atttatgtgg acattagaat gcattgtgtt
    10021 gtattttttt atagcctctc ttgaggccgt aaaacactaa caacgagcgg tcatcgagca
    10081 gcgcattcgg tccgctcgtc gtttcccccc ctcctccttt ctccttgact tttaccgcaa
    10141 aaataaacct ccatatccca tgaacataat cgccatcatc tcatcgacca tcctcctcct
    10201 caccaccgcc ctccccgccg cggcattcac tgcacgcgag ctcttcgtgg acacgggatg
    10261 ccacaccgcg cgggtatgga acatcgcgcg gtcaaacgac ggaacgcaga tcgtcacctc
    10321 gagccaggac aagaccctcc gcgtctgtga cgctaaaacg ctgcagccgg tccgaatcat
    10381 ccgcgggcag accggacagc acggctgggg catggagtac ggcgcgaaac tttttctgaa
    10441 cgacacgtat ctggtcaccc gcggcctgta cggcaccccg tacggcgccg ggcacgatat
    10501 ccgcgtgtat gattttaaga ccggaagaag ctattgctcc ctcttctccg acgacggcat
    10561 ggacaaggcc ttcgcgatct ccgaggacgg cgagcatctc atcgccggca atacaaaggg
    10621 cgagatctac gtcttcggta tgaaagagat tctagatcgc tcgcagaagc acccggtgct
    10681 gaggatcgat cgcgtcaacg gcgcgaagct caaggacgac tacatcaccg tgctgcacaa
    10741 gtaccgatcg gacccggcga agatcgatct taagcccgcg taccagccgg atcggacgag
    10801 cctcgtagag ctttccagtc acacgaaaga cggcgacgtc cgcatccttt cgatatcgaa
    10861 ggagtgcgtc tcgctctatt ccctgacacg ccggaccatg ctgaaatcgt ctcccctcaa
    10921 ggacgtgaaa aaagcgcgga tcacccggca ctacgcgatc gcgagcgaca gcgacggaat
    10981 caccatctat gacgcggagc tcaaccggaa agcgacgttc acgatggcgc acaaggtcca
    11041 cgatctgcgc atatccccgg acgaacgaac cctcgccatc atgctggcga atcgcgagtg
    11101 cgcgctctac gacatccgga gcggtgcgat acggaagctg cgctccgacg gccggctgga
    11161 cgtgtccgcc atggaattca tcgacaacaa gactcttgca ttcgccgacg agaacgcatt
    11221 caccctcttc gccgtcgaca cggccaccgg gaaggagctt ccccgctcgg ccggccttga
    11281 ggccctgccg atctggggcc tgggcgcgcg gggaagcgag atcgctctgg ggaccgagcc
    11341 gtacgtcgcg ccgggcgatc agccggagct cgaaatcgcg ttcgacctga agaccttcac
    11401 agcgagaccg gctgccggcg gagcgccctt tgcccccctg ccgctcgagc agggggtgtt
    11461 ccgcgcgaag tggaagcgcg gagagaagcg cgactcgatc gaactctacc gggggaacac
    11521 gctcgtctcc gcgatcacgc ccgacgagcg cacccacccg tggctgtgga cctacgggat
    11581 cacgccgtac ggcttcgtcg tcgcgggagg gcctggcggc acgctcctgc agtggctcac
    11641 cgtcacgtac gatgacggca accccgtgaa gggcgcctgc acagaccacg ccgccgccgt
    11701 cacgtcattc gcctgcagcg accggcacct cttctcggga agcatcgacc ggaccatcgt
    11761 gatatacgat ttacacaaga tgctggatgc gttccgcgcc gagctcgtca aagggaaaac
    11821 acgcgaagat tcgacggcgt cggacccgaa agggaaaaag gaggcggatg tcttcaccat
    11881 aaaatcgacc tactcgctgc ggaagctcat cagcccggag gtgcttgcgc gcgtcatgat
    11941 cggccggaac ggcgagtact gtatctggac gcccgacggc tatttcgcag ccagcgaggg
    12001 attcaggggc cgcgtcggga tcttcgacta caagggtgaa gggcgggagg cgacctggcg
    12061 gtcgggaaag gaatacttcg acgaccacca ccgccccgac atcatccgaa agcggttgga
    12121 attcgagtag gccacgtatc gatggcacat aacggctatt cgggttgccg gcgcatgaag
    12181 acagatgcca tccacaggag tatcccatga ccaccacggg cttccaggac cactaccccg
    12241 acgattacgc gcactgctac ggctgcggca ggctcaacga gcacggcatg cgcctgaaaa
    12301 gcgtctggga cggcccggag agcgtctgcc gcttcacgcc gcggccgcac cagacgggag
    12361 gcttgcggga catcgtgtac gggggcctca tcgcctccct cgtggactgc cactgcgcgg
    12421 ggaccgcggc cgccgcgaag ctccgcgagg aggggttcca gccgggagag cgccccatgc
    12481 cgcgcttcgt taccgcgtcg ctcaaggtcg actatatcgc ccccaccccc atcggcgtcg
    12541 agattgaggt gcgcgcgcgc gtcgtcgaga tgaagggccg caaggcggtc atcgccgcta
    12601 ccgtttccgc cggcggcacg atgaccgcga agggcgaggc ggtgatggtg cagctgcggg
    12661 agacggctag cgccacggaa gagcggtgag agcgctgtcg cgatcaacag cgccggaggg
    12721 ggccgcatcc ggcaaatagc gcttgcaaat atcgacggcc atgcaatact tcggtaacct
    12781 tttcccggca acggacatca cattcgaaac gcgacaccat accagataca cgagcatgaa
    12841 cataatcgcc atttcaaacc tttcgaagcg ctacggccga agcatccagg cgctcgcgga
    12901 cttctcgctc gccgtggagc gcggcaccat catggggctc atcggcccca acggcgccgg
    12961 gaagaccacg ctcatcaagt ccatcctggg cctggcgcac gccgacgcgg gggagatcgc
    13021 catcaacggc atcaccgggg ccgatcccgc tgcgcgcgcc tccatcggat tcctgcagga
    13081 gaagttcaac ttcttcccga cctacaccgc gcgaaacgcg ctcgccttct tcgcggcgct
    13141 caagaaaccg gcgggcgtcg cccatgtcga catcgccggc atgatcgaca cggtcgcgcg
    13201 caccgtcatg atcaccgagc tcctggaccg gaagatcaag accttctcga agggcgagct
    13261 ccagaaggtg ggcatcgcgc aggccctcct ctacgagccg gacctcctga tcctggacga
    13321 gccgttcagc gggctcgacc ccatcatcac caaggaactc aaggacatca tcctcgccta
    13381 ccgcgccaag ggcaaaacgg tctttttcag ctcgcacatc ctgggcgatg tcgcgcagct
    13441 ctgcgaccgg gtggccatca tcaacaaggg cgtgctggtg cgggaatgcg cggtgggcga
    13501 cctcaccggc gacctcacgc tcgaggacgc cttctacaat attatcacgg gaaaaaacta
    13561 gaaccgcatg aaccagatac tcgtcctcgt gcaaaacacc atcaagaagg agttcaccaa
    13621 caagctcctc atcttcatgt tcgccgtgac ggtgctcatc atctacgtgc tggggaacat
    13681 ctcggacttc gtcgtgaaaa acatttcaat gacggggccc ggcggccagc agtcggcgtc
    13741 ggcggtgtcc gcggcggccg gcgggtacag cttcgcggcc ttcttcttcc tcatccagtc
    13801 ctggaacgcg ctgctcgcgg tgctggtgac catcggcggc atcggcagcg actatcgcta
    13861 cggggttatc acgcagatcc tctcgcagcc gcttccgcgc tggaaatacc tggtgtcgcg
    13921 catcgcgggg tcctacctca tcatcctggc ctattacgtg atatcgctgg cgctggcgtt
    13981 cgtgttcctg tcggggaact tctcggccca gtcgctcacc ccgacgctcg cggggtcctt
    14041 cgccgtcgcc cagctcgtga tcctgttcta catcacggtg tcggtaatca tggccctgta
    14101 tgtcccgcag aagatcgcct tcttcctcgt gtttattctg gccctctttc ttcactacgc
    14161 gaattaccac gcgggggccc tgttggcaag cggggaaagc gtcctcgcga aaccggtgct
    14221 ggtcctggcc accttcgcct ttccgcagat cgggaccatc tcgaagttct ccagcttctc
    14281 cttcacgccc gacgcgagct actccgccgc ggaggtcctg ggccattcgg cgctcttcct
    14341 ggcgatgctt gccgcatggt tcgtcggggc gtgcttcgcg taccgccgca aggacatcag
    14401 ctgacgccgt tcccgcgccg cacatgcgac ggagcgcacc gagacgcaaa at
//
LOCUS       DAORHF010000080        14249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3320_length_14249_cov_5.910103, whole genome shotgun sequence.
ACCESSION   DAORHF010000080 DAORHF010000000
VERSION     DAORHF010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3320_length_14249_cov_5.910103"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PKJ16_08430"
     CDS             complement(<1..242)
                     /locus_tag="PKJ16_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47053.1"
                     /translation="MRIERNRFESMVRGTIRSVIDALPEDLRERAREVIFEIADRPTR
                     EQAEREGADDLLGLYEGTSLIDRHIDFPPVSPDRIY"
     gene            487..2346
                     /locus_tag="PKJ16_08435"
     CDS             487..2346
                     /locus_tag="PKJ16_08435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV47054.1"
                     /translation="MNWREEYRAKIQTADQALARLRSGQRVFVGSSCGEPQHLVDALL
                     GGAERLRDLEIVRLLSLEGSITSIYGDREYGHTFTVRSIYQGAGQSEFLSANWRFFAP
                     MDISLIPRLFAARKLRIDAALVQVSPPDEFGWMSLGISVDITRAGARAADYLVLQVNP
                     RMPRVLGQGFIHVSEAHAIVEHEEELLAVLEFPAPDASDAIAELIENRVDDGATIQLG
                     LGHGADAVIAALAAKRDLGVHTQYFTDGIMQLMNAGAVTNRRKTAHEGKSIASTAMGT
                     EDLYRFVHDNPGVEFHASDYINNPAVIASQHRMTAINFGTAIDLTGQIAIDALPQNHF
                     SGVTGVTDFVDGAVHSPGGRSIMVIPALTADGTSSRIVPGLAGGSVVIPRSDVYHVVS
                     EYGAVNLYGKNIEERAMALIGIAHPDHREALFDRARADGLIGPRVTLQQSLCGVYPAH
                     LEDAKTYGDAVIVFRPVRSGDARRLQEHFYSMEMTDVATRFFQPRASFYRDQIQSMFE
                     VDYRNHLTIVAIEGDADFGRIIGVGEYVIETGRNEAEVAFSVSKEWQGKGIAKELLVR
                     LTTAARENGIEGFRAFILPRNQAMIQLFRKLPFGIETAVDEDCLVLSCRFEAD"
     gene            2526..3749
                     /locus_tag="PKJ16_08440"
     CDS             2526..3749
                     /locus_tag="PKJ16_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47055.1"
                     /translation="MNMKKKKSTPLIACAVAAMVAAVLLGALAVACSGERALKSAGAG
                     KDFIDEARWIYVALACGDGPLPDGVDAAARARHCATLTDYAKRYRTNFIEKSRPFFDE
                     VVPRDAPGAVVYPFGGSDVCTTLATFPNATEITTISLENAGDPRMIPRDNHARQRTVM
                     AQVRKVVGTYLLAHDNSNTNIHDADRILLGGQLTFSLVAAVIFGLEPVSVRYFRIEPD
                     GALHYYTPDEISALEHVKGTRLHRAWVDLNYSVAFRNVEVVYRRKGVTGARGLLVHRH
                     MGANLHDSKFANSPLLAHLRKKGKVSAMTKAASYLLWYDSFSAVRNYLLQNMVFMISD
                     STGILPHHAQAAGFEQITYGSFNGAFLANRGGAAAAELRVFWKARNAGPLKFRYGYSD
                     DRGASHLLITRPRKS"
     gene            complement(3727..5715)
                     /locus_tag="PKJ16_08445"
     CDS             complement(3727..5715)
                     /locus_tag="PKJ16_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47056.1"
                     /translation="MTLHELARFLRSLRGALNRRTLSFRLIVLMLSTTGMIFIVAFAY
                     NYVATSAIMLEDATINARHLAQSVIGRIAAHSDTIQKLTKSIAAQLGARQDLPWLYTT
                     QRQLVENNNDIFGTCAAFEPFSADPGRRHFAPYHFKRDGSVSLKVLGGTGYDYFAMPW
                     YAHPKETGQPGWTGPYYDEGGGNVLMATYSVPFYRTVNGAQRFAGVVTVDISIDWLSD
                     YLNGIRLFESGYAFLIMRDGTFITHPDRRLVSRENMFTRIAQSGDPSLARIIKRMDVE
                     PFGAAFGRDFHTGKRSLHYIHSLAATGWIIVIVVPEHELYGDLRALARTIVLIIIVGM
                     LALFVAVLFVARSITRPVHSLSRVAGEIARGNLDIDVPHADLANEIGMLAESFRSMKL
                     SLREYIANLRETTAAKERIESELAVGRTIQMSFIPAPPRPADRREEYEIAAAIEMARE
                     VGGDLYDIVPLDEDRLFLLVGDVSGKGVPAALLMAVTRTLFRAFATSYRRPAALLERV
                     NEELARENDSAMFVTAFCGVYNCATGELAYSNAGHNPPLFMGADGDIRPLAIPGGIAL
                     GADDGAAFGDAIIAMRPGERILVYTDGVTEALNERGEFFGEPGLIRALARAHSASAEE
                     TVARTIAAVREHAGGAPQSDDITLLCLRALRITTSADA"
     gene            complement(5712..6155)
                     /locus_tag="PKJ16_08450"
     CDS             complement(5712..6155)
                     /locus_tag="PKJ16_08450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47057.1"
                     /translation="MKPLPRDRDAAIRLPATDASIETAGARVDAILSALGVSRERALR
                     IELALEELLVNIVSYAYPADGPGDFEILIAPSADAITLTVTDWGRPFNPLEADPPRTG
                     DGIDDREPGGLGIHLVRELARSVRYERAGDANRIAFTFPTGGTSG"
     gene            complement(6130..6465)
                     /locus_tag="PKJ16_08455"
     CDS             complement(6130..6465)
                     /locus_tag="PKJ16_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47058.1"
                     /translation="MDVSISTQEGRAILRVAGRMDATTAPQFERECAALIESGERHLV
                     AELSDLEYISSAGLRIFLATAKKLKPLEGEIRICGMSGLVREVFSLSGFISLFAIYPS
                     LDEALAARP"
     gene            6600..7673
                     /locus_tag="PKJ16_08460"
     CDS             6600..7673
                     /locus_tag="PKJ16_08460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HNV47059.1"
                     /translation="MADKRALITGIRGQDAAYLARLLLEEGCAVFGADKKSGDTTYWR
                     LKRMGVFDDVRVVYMDMTDQANIIATLEAVRPDEIYNLAAQSFVENSFRQPLLTTDVN
                     AAGTLRLLEAIRLTHLPARFYQASSSEMYGASREIPQTELTPFYPRSPYGVSKLFAHW
                     ITVNYRESYTIHACCGILYNHESPLRNPEFVTRKITRAAARIARGLQDELVMGNIDVR
                     RDWGFAGDYADGIRRIMRHPVPDDYILATGESHSIREFIDEAFAAAGIDIDWEGTGAS
                     ARGVERKTGRAIVRVSDEFFRPADIAIVQGDAAKARTVLGWEPRVAFRELVRLMVESD
                     LREADEDRAQGDGTSGASRPHPR"
     gene            complement(7699..8367)
                     /locus_tag="PKJ16_08465"
     CDS             complement(7699..8367)
                     /locus_tag="PKJ16_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47060.1"
                     /translation="MHTRVARTACALALVMALFSACATTRLTAVWKDQRYAGGPFSTI
                     AVIALFERLSNRQTFETLLVERLRASGTGAVAGLDFMAPDQRYDYAALEKKFSALGVD
                     GILIVRLTSVERKQVYVPGGDTVFPETRYDKYANYYYETYRQACDAGQVKETDIFIIE
                     SDLYANAGDRLVWRAETRSYEPGERAAEPMNAPAIASELGARIVQILREHSLIAPASA
                     KKSN"
     gene            8568..9968
                     /locus_tag="PKJ16_08470"
     CDS             8568..9968
                     /locus_tag="PKJ16_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47061.1"
                     /translation="MNESNTDKPLEIYYGKRKTDILPGVIVDDMGRRCVKAAHYASMN
                     IQIRKLSMLMDIARSIMAEMDLTTLLQLVMKTVTQVMNADRSSLFLVDRDTGEIWSKV
                     AQGVSEIRLPMGTGIAGHVAQTGETVNIPDAYLDPRFNRDSDERTGYRTQTILCMAIT
                     NPRGEIIGSIQVLNKLDGTSFSADDEELLGAFCSLAGISIENAWAYEELEKERASLEV
                     KVRERTRDLQIAKQKSDDLLRNILPDETAEELKSRGVTTPHYYERVTVLFTDFKGFTH
                     VAEKLNPADLVKELDLCFYYFDEVSERNKLEKIKTIGDSYMCAGGLPAANRTNPVDAI
                     LAALEIRNFIEQTRAIKRTLGEKFWELRIGIHTGPVIAGVVGKKKFAYDIWSDTVNTA
                     SRMESSGVVGEINISGATYELVKDFFVWDYRGKIPAKHKGDIDMYLVTRIRPELSADA
                     EGCVPNDRFRELYAML"
     gene            complement(10152..10775)
                     /locus_tag="PKJ16_08475"
     CDS             complement(10152..10775)
                     /locus_tag="PKJ16_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HNV47062.1"
                     /translation="MTTSDRLPLAFLAASIALLATSLVLSLDRFTWILEVFPILIGLP
                     LLVATYRRFPLTTLLYALLIIHFCILAVGGIYTYAKVPLGFWMQEAFGFARNHYDRIG
                     HFAQGFIPAILTREILIRTSPLRPGKLLYFIVVCVCLAISAFYELIEWWVTAAQGASA
                     EAFLGTQGDVWDAQWDMFFCLIGANVALIVLSRFHDRQLRNMGHLSD"
     gene            10951..12384
                     /locus_tag="PKJ16_08480"
     CDS             10951..12384
                     /locus_tag="PKJ16_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV47063.1"
                     /translation="MRCLRIVSMVVALVAFPVSRATAGAGANAPLDLGGAIGEAMKHN
                     NALKIALEKSRESRARVREAWGMLWPELSTDVSYTAMRSDAGLNAEIAGVSDIRYVNG
                     RFAVNPGLFYNSLQGSRAAHIAADNEVRRIRTDTVTKTIGLFYDLVLAVENVAMRRET
                     VKALEENLRVLTVGYRAGTASKLEFLRANVSCANERTRLIIAENALVNAQAALNIQLG
                     RDVFAAIATAGDIRAAFVDGVAEKTVRDSEQEAFVRGMIGAALRSRPEIIQVKMKRKI
                     RLHEKGAAESVFLWPTFFAQGSYGATSYQRRDSGTSWGGSGDPARDALLRSLGEALAD
                     TISPTGWNSAWQVTFGATYRWGALSPLDSSHARADQAASRHRQADYEMEDFIKAVKLD
                     VRQGFLKLKSAYVAIKAQEGNIVMAEESVRVAGLQYRNGIIDNQKFLDATAELATAKT
                     YYIQALHDFQVAKAEINRAVGRDVFSF"
     gene            complement(12400..12864)
                     /locus_tag="PKJ16_08485"
     CDS             complement(12400..12864)
                     /locus_tag="PKJ16_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47064.1"
                     /translation="MRIETTININIETLKRIEAACHTLRISRSRLIAMLIECAMRERR
                     DTSRMCRRVRYQERLDPAQWRKLHITLDAALYECMIDLRKIDKVSVSFILALSVQKHI
                     TALLLKGMNTNKTDNYPIINYIFSKTIVGGVISWRVFWGIPTNLPTLACEFT"
     gene            complement(12955..>14249)
                     /locus_tag="PKJ16_08490"
     CDS             complement(12955..>14249)
                     /locus_tag="PKJ16_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV47065.1"
                     /translation="KDPDIVNFASYVGQGSPRFVLTFNPTLPASNFGQLVILAKDGKA
                     RERLTRKIEKIFAQSFPSVNARIKILPNGPPAQYPVMFRVSGYGHDTVKETAEALREI
                     MLRNKKISMVEFDWYQKNRVLALDIDLDKARLMGIDTRELALALNAQISGLGVAEFHE
                     RDKLIGIVVRMEPKDRRDLSRLKDLVVPAHGGYIPLAQIARLKYESEEALVWRRNLIP
                     TITVGADIVPGVTGNDVSAEIMKAGAALRAKLPFGYAIQVAGNAEDSEKSQGHLAKTY
                     PAMIVVIMILLMVQLQNIPRMIMVLLTAPLGLIGVNVFLAALRKPMGFVALLGVIALF
                     GMIMRNSVILIVQIEKHIAEGQRPWDAIIESTLLRFRPIILTAAAAILGMIPLVRSEF
                     WGPMAVAIMGGLFVATALTLLYLPALYALWFKVRESKY"
BASE COUNT         2552 a         4416 c         4794 g         2487 t
ORIGIN      
        1 atgtagatgc ggtcggggct caccggcggg aagtcgatat gccggtcgat gagggaggtc
       61 ccttcataaa gccccaggag atcgtcggcg ccctcgcgct cggcctgctc gcgcgtgggc
      121 cggtccgcga tctcgaagat cacctcgcgc gcgcgctcgc gcaggtcctc cggcagggcg
      181 tcgatgacgc ttcggatggt cccgcggacc atcgactcga atctgttccg ttcaatccgc
      241 atggcatgtc ccgatggcgc aatcggagga catcgcatgc ggtatttcaa gaactttccg
      301 atcggcgcgc gaagggattg tcggaaaatg gttgcttttt tttcatcgcc cgtcatggtc
      361 gatccggctt tcccgtcccc gctcgtggag gtggcaccgc atgaaaatct gtcccgtctg
      421 cacgcacgtt attatcgaag atgacgcgcg gtgccgcacg aaatgcgggg cgcgccgatg
      481 gggccgatga actggcgcga ggaataccgg gcgaaaatcc agacggccga ccaggccctt
      541 gcgcgcctgc gctcggggca gcgcgtcttc gtgggatcgt cgtgcggcga gccgcagcac
      601 ctggtggacg cgctcctcgg aggcgccgag cgcctgcgcg atctcgagat cgtgcgcctc
      661 ctgagcctgg agggctccat cacctccatt tacggcgacc gcgagtacgg gcacaccttc
      721 acggtgcgca gcatctatca gggcgccggg cagtcggagt tcctgagcgc caactggcgc
      781 ttcttcgcgc ccatggacat ctcgctcatc ccgcggttgt tcgccgcgcg caagctccgt
      841 atcgacgccg cactcgtgca ggtctcgccg ccggacgagt tcggctggat gagcctgggc
      901 atctccgtgg acatcacccg cgccggggcc cgcgcggccg actatctcgt gctgcaggtg
      961 aacccgcgta tgccgcgcgt cctggggcag gggttcattc acgtaagcga ggcgcacgcg
     1021 atcgtggagc acgaggagga gctgctcgcg gtgctggagt tccccgcgcc cgacgcgagc
     1081 gacgccatcg ccgagcttat cgagaaccgg gtcgacgacg gcgccaccat acagctcggg
     1141 ctcggccatg gcgcggacgc ggtcatcgcg gcgctcgccg cgaagcgcga tctcggcgtc
     1201 cacacgcagt acttcaccga cggcatcatg cagctcatga acgccggcgc cgtcaccaac
     1261 cgccggaaga ccgcacacga gggcaagtcc atcgcgagca cggcgatggg caccgaggac
     1321 ctctatcgct ttgtgcacga caacccgggc gtggagttcc acgcgtccga ctatatcaac
     1381 aatcccgccg tgatcgcctc gcagcaccgc atgaccgcca ttaatttcgg gacggccatc
     1441 gacctcaccg ggcagatcgc gatcgacgcg ctcccgcaga accacttctc cggcgtcacg
     1501 ggcgtcaccg acttcgtgga cggcgcggtg cattcgcccg ggggccgctc catcatggtg
     1561 atccccgcgc tcacggccga cgggacgtcc tcgcggatcg tgccggggct cgcgggcggt
     1621 tcggtggtga tccctcgcag cgacgtctac cacgtggtga gcgaatacgg cgcggtgaac
     1681 ctctacggca agaacatcga ggagcgggcg atggcgctca tcggcatcgc gcatcccgac
     1741 caccgggagg cgctcttcga tcgcgcgcgc gccgacgggc tcatcggccc gcgcgtcacg
     1801 ctgcagcagt cgctgtgcgg cgtgtacccc gcgcacctcg aggacgccaa gacctatggc
     1861 gatgccgtca tcgtcttccg gccggtgcgg tcgggcgatg cgcggcgttt gcaggagcac
     1921 ttctattcca tggagatgac cgacgtggcc acgcgcttct ttcagccgcg cgcctcgttc
     1981 taccgcgacc agatccagag catgttcgag gtcgattacc gcaaccacct caccatcgtg
     2041 gccatcgagg gcgacgcgga cttcgggcgc atcatcggcg tgggggagta cgtgatagaa
     2101 accgggcgca acgaggccga ggtcgccttt tcggtctcga aggagtggca gggcaagggg
     2161 atcgcgaaag agctccttgt gcggctcacg accgccgcgc gggagaacgg gatcgagggc
     2221 ttccgcgcct tcatactgcc gcgcaatcag gcgatgatcc agctcttccg gaaacttccg
     2281 ttcggcatcg agacggccgt tgacgaagac tgcctggtcc tgtcgtgccg cttcgaggcc
     2341 gactgacgtg gcgcgcgctc gatcgtcggg cgtccccgcg ggcggttcca cggcgcgttc
     2401 gtttccgctc tcgtcgaagg agggaacgcg aactgaatac accatgatcg ccgccgtcgt
     2461 gcgcgtgtcg ctgcggaggc ggatgggttt tctatacact ttacgacatg aaggatgatt
     2521 gctccatgaa catgaaaaaa aagaaatcaa ccccactcat cgcgtgcgcc gtcgcggcga
     2581 tggtcgccgc cgtgctgctg ggagcgcttg ccgtggcctg ttccggggag cgcgcgctca
     2641 agagcgccgg cgccgggaag gacttcatcg acgaggcgcg ctggatctac gtcgcgctgg
     2701 cctgcggcga cggccccctg cccgacggcg tggacgcggc ggcccgcgcg cggcactgcg
     2761 ccacgcttac ggattacgcg aaacggtatc ggacgaactt tatcgaaaag tcccgcccct
     2821 tcttcgacga ggtggtgccc cgcgacgcgc cgggggccgt cgtgtacccg ttcggcggga
     2881 gcgacgtgtg cacgacgctc gcgacgttcc cgaacgcgac ggagatcacc acgatctcgc
     2941 tggagaacgc gggcgatcct cgcatgatcc cccgggacaa ccacgcccgc cagcgtacgg
     3001 tgatggcgca ggtgcggaag gtcgtgggca cgtatctcct cgcgcacgac aacagcaata
     3061 ccaatatcca cgacgccgac cggatcctgc tgggcgggca gctcaccttt tcgctcgtcg
     3121 cggccgtcat cttcggcctg gagccggtgt cggtgcgcta tttccggatc gagcccgacg
     3181 gcgcgctgca ctattatacc cccgacgaaa tttccgcgct ggagcacgtc aagggcacgc
     3241 ggctgcaccg cgcctgggtg gacctcaact attcggtcgc gttccggaac gtggaggtgg
     3301 tgtaccggcg taaaggcgtg accggcgccc gcgggctcct ggtgcaccgg cacatgggcg
     3361 ccaacctgca cgactcgaaa ttcgcgaact cgccgcttct ggcgcacctg cgaaagaagg
     3421 ggaaggtgtc cgccatgacc aaggccgcga gctatctgtt gtggtacgat tcgttctcgg
     3481 cggttaggaa ctacctgctg cagaacatgg tcttcatgat ttccgattcg accggcatcc
     3541 tgccgcatca cgcgcaggcc gcggggttcg agcagatcac ctacggctcg ttcaacgggg
     3601 cctttctcgc caatcgcgga ggcgcggcgg cggcggagct gcgggtgttc tggaaggcgc
     3661 gcaacgcggg gccgctcaag ttccgctacg gctactcgga cgaccggggc gcgagccacc
     3721 tactcatcac gcgtccgcgg aagtcgtgat ccgcagcgcg cgcaggcaca gcagggtgat
     3781 gtcgtcggac tgcggcgcgc cgccggcgtg ctcgcggacg gcggcgatcg tccgggcgac
     3841 cgtttcctcc gccgaggccg agtgcgcgcg cgcgagcgcg cggatcagcc ccggctcccc
     3901 gaagaattcc ccgcgttcgt tgagcgcctc ggtcacgccg tcggtgtaga cgaggatgcg
     3961 ctcgccgggc cgcattgcga tgatcgcgtc gccgaacgcg gcgccgtcgt cggcgcccag
     4021 cgcgatcccg ccgggaatcg cgagcggacg gatgtcgccg tctgcgccca tgaacagcgg
     4081 cggattgtgc cccgcgttcg aataggcgag ctcgcccgtc gcgcaattgt acacgccgca
     4141 aaacgccgtg acgaacatcg ccgagtcgtt ctcgcgcgcg agctcctcgt tgacgcgctc
     4201 caggagcgct gcgggtcgcc ggtagctcgt cgcgaaggcg cgaaagagcg tgcgggtgac
     4261 cgccatcaag agcgccgccg ggacgccctt gccggacacg tcgcccacga gtagaaagag
     4321 ccggtcctcg tccagcggga caatgtcgta gagatcgccg ccgacctccc gcgccatctc
     4381 gatggccgcc gcgatctcgt actcctcgcg gcggtcggcg ggccgcggcg gggcgggaat
     4441 gaagctcatc tggatggtgc ggcccaccgc gagctcgctc tcgatgcgct ccttggccgc
     4501 cgtggtctcc cggaggttcg cgatgtattc gcgcagcgag agcttcatgg agcggaagga
     4561 ctcggcgagc atcccgatct cgttggcgag gtccgcatgc ggcacgtcga tgtcgaggtt
     4621 gccgcgggcg atctcgccgg cgacgcgcga gagcgaatgg acgggccgcg tgattgagcg
     4681 cgccacgaac agcacggcca cgaacagggc cagcatcccg acgatgatga tgagaacgat
     4741 cgtgcgggcg agggcgcgca ggtcgccgta gagctcgtgt tccggaacga cgatcacgat
     4801 gatccagccg gtggccgcga gcgaatggat gtaatgcagg gagcgcttcc cggtgtggaa
     4861 gtcgcgtccg aacgcggcgc cgaacggttc gacgtccatg cgtttgatga tccgcgcgag
     4921 agacgggtcg ccgctctgcg cgatgcgggt gaacatgttt tcgcgcgaga cgagccgccg
     4981 atcgggatgg gtgatgaacg tgccgtcgcg catgatgagg aacgcgtatc ccgactcgaa
     5041 gaggcggatg ccgttgagat agtcggacag ccaatcgatt gagatgtcga ccgtcacgac
     5101 gccggcgaat cgctgcgcgc cgttgacggt gcgatagaag gggacggaat aggtggccat
     5161 gagcacgttt cccccgccct cgtcgtagta ggggccggtc cagccgggct gtcccgtttc
     5221 cttcgggtgt gcgtaccagg gcatggcgaa ataatcgtat ccggtcccgc cgagcacctt
     5281 cagtgacacg gagccgtccc gcttgaaatg atagggtgcg aagtgtcgcc gcccggggtc
     5341 ggcggagaac ggttcgaagg cggcgcaggt gccgaagatg tcgttgttgt tttccaccag
     5401 ttgccgctgc gttgtgtaca gccatggcag gtcctggcgc gcgccgagct gcgcggcgat
     5461 gcttttcgtg agcttctgaa tcgtgtccga gtgcgccgcg atgcggccga tgaccgactg
     5521 cgccaggtgc cgggcgttga tcgtcgcgtc ctccagcatg atcgccgagg tggcgacata
     5581 gttgtaggcg aaggcgacga tgaagatcat cccggtggtg gagagcatca ggacgatcaa
     5641 tcggaaggac agggtgcggc gattgagggc cccgcgaagc gatctgagga accgggcgag
     5701 ttcgtgaagg gtcatcccga tgttccgccg gtcgggaagg tgaatgcaat gcgattggcg
     5761 tcgccggcgc gttcgtagcg gacgcttcgc gccagctcgc gcaccaggtg gattcccagg
     5821 ccgcccggtt cccggtcgtc gatgccgtcg ccggtgcggg gcggatcggc ctcgagcgga
     5881 ttgaagggcc gtccccagtc ggtaacggtg agggtgatcg cgtccgccga cggcgcgatg
     5941 aggatctcga aatcgcccgg accgtcggcg ggataggcgt acgacacgat gttcacgagc
     6001 agctcctcga gggcaagctc gatacgcagc gcacgctccc gagaaacgcc cagtgccgac
     6061 aggatcgcat cgacgcgcgc gccggccgtc tcgatcgatg cgtccgtcgc cggaaggcga
     6121 atcgcggcgt cacggtcgcg cggcaagggc ttcatcgagc gagggataga tcgcgaagag
     6181 cgaaatgaac cccgaaaggg agaagacctc ccgcaccagg ccgctcatgc cgcagatgcg
     6241 gatctccccc tcgaggggct tgagcttctt tgcggtcgca agaaagatcc gaaggcccgc
     6301 gctcgatatg tactcgaggt cggagagctc ggccaccaga tggcgctcgc cggactcgat
     6361 gagcgcggcg cactccctct cgaactgcgg ggcggtggtc gcgtccatgc gccccgcgac
     6421 gcgcaggatg gcgcgcccct cctgtgtgct gatgctgacg tccatggtcg tcgtcctcct
     6481 gtgcgctgct gtgatcgaag gctagtgcgc gtaaatgaaa aatcaatcca atttttttcc
     6541 gatcgcgccg cggccaagaa aaggattgcc atcgccgcgc gggacggcta caggggatca
     6601 tggctgataa acgggcgctc atcaccggga tccgcgggca ggacgcggcc tacctcgcgc
     6661 ggctcctcct ggaggagggg tgcgcggtgt tcggggccga caagaagagc ggcgacacca
     6721 cctactggcg gctcaagcgg atgggcgtgt tcgacgacgt tcgcgtggtc tacatggaca
     6781 tgaccgacca ggcgaatatc atcgcgacat tggaggcggt gcgcccggac gagatctaca
     6841 acctcgccgc gcagagcttt gtcgagaact ccttccgcca gccgcttctg accacggacg
     6901 tgaatgcggc cggcacgctg cggttgctcg aggcgatccg cctcacgcac ctgcccgcgc
     6961 gcttctacca ggcgtcctcc agcgagatgt acggcgcgtc gcgcgagatc ccgcagacgg
     7021 agctcacgcc gttttatccg cgcagcccct acggcgtctc gaagcttttc gcgcactgga
     7081 tcaccgtgaa ctaccgcgaa tcatacacca tacacgcctg ctgcggcatc ctgtacaatc
     7141 acgagagtcc gctgcgcaat cccgagttcg tgacgcgcaa gatcacccgc gcggcggccc
     7201 gcatcgcgcg cggcctgcag gacgagctgg tcatgggcaa tatcgacgtg cggcgcgact
     7261 gggggtttgc gggcgattat gccgacggca tccggcgcat catgcggcat cccgtcccgg
     7321 acgattatat cctggcgacc ggcgagtcgc actcgatccg cgagttcatc gacgaggcct
     7381 tcgcggcggc cggcatcgac attgactggg aggggacggg cgcgagtgcg cggggcgtcg
     7441 agcgaaaaac ggggcgggcg atcgtgcgcg tgtcggacga gtttttccgg ccggccgaca
     7501 tcgccatcgt gcagggcgat gccgccaagg cgcgcacggt gctcggctgg gagccgcgcg
     7561 tcgccttccg ggagctcgtg cgcctcatgg tggaaagcga tctgcgcgag gcggacgagg
     7621 atcgcgcgca gggcgacggg acatcgggcg cgtcgcggcc gcatccccgg tagcgatcgc
     7681 gcgcttcgac gggcgctcct agttcgattt cttcgccgag gcgggcgcaa tgagggagtg
     7741 ttcgcgcagg atctggacga tacgcgcgcc cagctcgctc gcgatcgccg gcgcgttcat
     7801 gggttccgcg gcccgctcgc ccggctcgta gctgcgcgtc tccgcgcgcc acacgagccg
     7861 gtcgcccgcg ttcgcataca ggtcgctctc gatgatgaag atgtcggttt ccttcacctg
     7921 tcccgcgtcg catgcctgcc ggtaggtctc gtagtagtag ttcgcgtatt tgtcgtagcg
     7981 cgtttccggg aacaccgtat cgccgcccgg gacataaacc tgctttcgct ccaccgaggt
     8041 gaggcgcacg atgaggatcc cgtcgacgcc gagggcggag aactttttct ccagcgcggc
     8101 gtagtcgtac cgctggtcgg gcgccatgaa gtcgagcccg gcgaccgcgc cggtcccgga
     8161 cgcgcgcaat cgctcgacga gcagcgtctc gaaggtttgg cggttggaga ggcgctcgaa
     8221 gagcgcgatc accgcgatcg tggaaaacgg accgcccgca taccgctggt ccttccagac
     8281 ggccgtgagc cgcgtggtgg cgcatgcgga gaacagcgcc atgacgagcg cgagagcgca
     8341 tgcggtgcgg gctacgcggg tgtgcatcgt gctcctccgg tgatggtgtc gccgtatccg
     8401 atcacgcgga agcgcacctg tcaagcagga tgcggtcgtg gcggcgcatt gtgtgatgat
     8461 ggttccggcg gcggggcaat cgtgtcgccg ggtatttttc ccatgcagaa tcggcctcgc
     8521 ccggccgata ggtagtgtat acctccttgc gagcgcgacg cggacatatg aacgaatcga
     8581 acaccgacaa gcccctcgag atatactacg gaaagcgcaa gaccgacatt ttgccgggcg
     8641 tgatcgtcga cgatatgggg cgccgctgcg tgaaggcggc gcattacgcc agcatgaaca
     8701 tccagatccg caagctctcg atgctgatgg acatcgcgcg ctccatcatg gcggagatgg
     8761 acctgaccac cctgctgcaa ctggtgatga agaccgtgac gcaggtgatg aacgccgacc
     8821 gctcgtcgct gtttctggtg gaccgggaca cgggcgagat ctggtccaag gtggcgcagg
     8881 gcgtttcgga gatccgtctc ccgatgggaa cggggatcgc gggacacgtc gcgcaaaccg
     8941 gcgagaccgt caatatcccg gacgcctacc tcgatccgcg cttcaatcgc gattccgacg
     9001 agcgcaccgg ctatcgcacg caaaccatcc tgtgcatggc gatcacgaac ccgcgcggcg
     9061 agatcatcgg ctcgatccag gtgctcaaca agctcgacgg cacctcgttt agcgccgacg
     9121 acgaggagct gctgggggcc ttttgctcgc tggcgggcat ctcgatcgag aacgcctggg
     9181 cctacgagga gctcgagaag gagcgggcct ccctggaggt gaaggtgcgc gagcgcacgc
     9241 gcgacctgca gatcgccaag cagaagtcgg acgatctgct gcgcaacatc cttcccgacg
     9301 agaccgccga ggagctcaag agccgcggcg tgaccacgcc gcactactac gagcgcgtga
     9361 cggtgctctt cacggacttc aagggattta cgcacgtcgc cgagaagctc aatccggccg
     9421 acctggtcaa ggagctggac ctgtgcttct actacttcga cgaggtttcc gagcgcaaca
     9481 agctcgaaaa gatcaagacc atcggcgatt cgtacatgtg cgcgggcggc ctccccgcgg
     9541 cgaaccgcac caatcccgtg gacgccatcc tggccgcgct cgagatccgg aatttcatcg
     9601 agcagacgcg cgccatcaag cgcacgttgg gcgagaagtt ctgggagctg cgcattggca
     9661 tccatacggg gccggtgatc gcgggggtcg tgggcaagaa gaagttcgcc tacgacatct
     9721 ggagcgacac ggtgaacacc gcgagccgga tggagtccag cggcgtggtg ggcgagatca
     9781 atatctccgg cgcgacctac gaactggtga aggacttctt tgtgtgggat taccggggga
     9841 agatcccggc gaagcacaag ggcgatatcg acatgtatct ggtgacccgc atccgtcccg
     9901 aactgtcggc cgacgccgag ggatgcgtcc ccaacgaccg cttccgcgag ttatacgcga
     9961 tgctgtaatc gcgatcgtcg cgacggtcag tggcttggtt gatccactcg atgtcgtcgc
    10021 gggcgatgcg atgtgctgca ataccgcgct ccatgcggat catacttgaa acaggattgt
    10081 gtgaagatgg cgccgggatg cttcgcttcc atcgcttcac gcaataccgc gccgcccggc
    10141 ctgcgggggc gtcaatccga caggtgcccc atgttccgca gctggcgatc gtgaaatcgc
    10201 gataggacga tgagcgcgac gttggcgccg atgaggcaga agaacatgtc ccactgcgcg
    10261 tcccacacgt cgccctgcgt ccccaggaag gcctccgcgc tcgcgccctg cgccgccgtg
    10321 acccaccact cgatgagctc gtagaaggcg cttatcgcca gacacacgca cacgacgatg
    10381 aaatacagga gtttccccgg ccgaagcggc gatgtccgga tgaggatctc tcgcgtcagg
    10441 atcgcgggga tgaagccttg cgcgaagtgg ccgatgcggt cgtagtggtt gcgcgcgaac
    10501 ccgaaggcct cttgcatcca gaacccgagc gggaccttcg cataggtgta gatgccgccc
    10561 acggccagga tgcagaagtg gatgatgagc agcgcataga gaagtgtggt gagcgggaag
    10621 cgccggtatg tcgcgacgag tagcggcagt ccgatgagga tggggaaaac ctcgaggatc
    10681 cacgtgaagc ggtcgagcga caaaacgaga gaggtcgcga ggagtgcgat ggacgcggcg
    10741 aggaaggcaa gcggcagcct gtcggatgtg gtcatggatg tcctccattc ggtgcggtgg
    10801 caaaggctac caggcgtcgt gtgcgatgtc aacgcttgtg cgcggaggaa cgacaaatta
    10861 ttgattgaat ttttttcatt gccgcacgat cgtgaaatgg cgcgcgccag tgtcgccgcg
    10921 ccgatgcttg attcccaggg tgcagccgtc atgagatgtc tccgtatcgt ttcgatggta
    10981 gtggcgctgg tcgcgtttcc ggtttcccgc gcgaccgcgg gcgccggcgc gaatgcgccg
    11041 ctcgacctgg gaggcgccat cggggaggcc atgaaacaca acaatgccct caagattgcg
    11101 ctcgagaaat cgcgcgagag ccgcgcccgg gtgcgcgagg cctgggggat gctgtggccg
    11161 gagctttcaa cggacgtgtc gtacaccgcg atgcgctccg atgcgggctt gaacgccgag
    11221 atcgccggcg tgagcgacat ccggtacgta aatggccgct tcgcggtgaa ccccggcctg
    11281 ttttacaact ccctgcaggg atcgcgcgcg gcgcacatcg cggcggacaa cgaggtgcgt
    11341 cggatacgca ccgacaccgt caccaagacc atcggcctgt tttatgacct cgtgctcgcc
    11401 gtggaaaatg tggccatgcg gcgcgaaacc gtcaaggcgc tcgaggaaaa cctccgggtg
    11461 ctcacggtag ggtatcgggc ggggaccgcg agcaagctcg agttcctgcg ggcgaacgtc
    11521 tcgtgcgcga atgagcgcac gcgcctgatc atcgccgaaa acgcgctcgt gaacgcgcag
    11581 gccgcgctca acattcagct cgggcgggac gtgttcgcgg cgatcgcgac cgcgggcgat
    11641 atccgcgcgg ccttcgtcga cggcgtggcc gagaagaccg tgcgcgattc ggagcaggag
    11701 gcgttcgtgc gcggaatgat cggcgcggcg ctgcgcagcc gcccggagat catccaggta
    11761 aaaatgaagc gaaagatcag actgcacgaa aaaggggccg cggaatcggt gtttctgtgg
    11821 cccacgtttt tcgcccaggg aagctatggc gcgacaagct atcaacgccg ggattcgggg
    11881 acgagctggg gaggatcggg cgatcccgcg cgcgacgcgt tgctgagatc gcttggcgag
    11941 gccctggccg acacgatcag cccgacggga tggaacagcg cctggcaggt gaccttcggc
    12001 gcgacctatc gctggggggc gctctcgccg ctcgattcgt cgcatgccag ggcagaccag
    12061 gccgcgagcc gtcaccgtca ggcggactac gagatggagg atttcatcaa ggccgtgaag
    12121 ctcgacgtgc gccaggggtt cctgaagctg aaaagcgcct atgtcgccat caaggcccag
    12181 gagggaaaca tcgtgatggc ggaggagtcg gtgcgcgtgg ccgggctgca gtacaggaac
    12241 ggcatcatcg acaaccagaa gtttctcgat gcgaccgccg agctcgcgac cgccaagacc
    12301 tactacatcc aggcgctcca tgatttccag gtcgcgaagg ctgagatcaa ccgcgccgtg
    12361 gggagggacg tgttttcgtt ttagatgaat gatcgcggct caggtgaact cgcatgcgag
    12421 ggtggggagg ttggttggga ttccccagaa aacacgccac gagataacgc cgccgacgat
    12481 tgttttcgaa aagatgtaat tgatgattgg atagttatcg gtcttgtttg tgttcatgcc
    12541 cttcaggaga agggcggtaa tgtgcttttg cactgagagc gccagaataa acgaaaccga
    12601 aaccttgtcg attttccgca gatcaatcat gcactcatag agcgccgcat ccagcgttat
    12661 atgcagtttt cgccattgcg cgggatccag gcgctcttgg tagcgtactc gccggcacat
    12721 tcgtgaggtg tcccgacgct cccgcatcgc gcattcaatg agcattgcaa tcaggcgcga
    12781 tcgcgagatg cgcagcgtat ggcacgccgc ttcaattcgc ttcagggttt cgatgttgat
    12841 gttgatggtc gtttcaatcc gcatggcgtc ctcccggtcg atagttatcg gtcattacat
    12901 ttgatacgat cggggatgtg gattttattg atgaaaatcc cgacaaattc gggatcaata
    12961 cttcgattcc cgcaccttga accagagcgc gtagagcgcc gggaggtaaa gcaaggtgag
    13021 cgccgtcgcg acgaagaggc cgcccatgat ggcgacggcc atcggccccc agaactcgct
    13081 gcggacgagg gggatcatgc ccaggatggc ggccgcggcg gtgaggatga tgggccgaaa
    13141 gcgcagcagg gtggactcga tgatggcgtc ccagggacgc tggccctccg cgatgtgctt
    13201 ttcgatttgc acgatgagga tgaccgaatt gcgcatgatc atcccgaaga gcgcgatcac
    13261 cccgagcagc gcgacgaagc ccatgggctt tcgcagcgcc gccaggaaca cgttcacgcc
    13321 gatgaggccc agcggcgccg tgaggagcac catgatcatg cgcgggatgt tctggagctg
    13381 gaccatcagc agaatcatga tcaccacgat catcgcgggg taggttttcg cgaggtggcc
    13441 ctgcgacttc tcgctgtcct ccgcgttgcc cgcaacttga atggcgtaac cgaacggcag
    13501 cttcgcccgc agcgccgcgc ccgccttcat gatctcggcg ctcacgtcgt tgccggtcac
    13561 gccgggcacg atgtcggccc ccacggtgat ggtggggatg agattgcggc gccacacgag
    13621 cgcctcctcg gactcgtatt tcagccgcgc gatctgcgcg agcggaatgt agcctccgtg
    13681 cgcgggcacc accaggtcct tgaggcgcga gaggtcccgc cggtccttgg gctccatgcg
    13741 aaccacgatg ccaatcagct tgtcgcgctc gtggaactcg gccaccccca gtcccgatat
    13801 ctgggcgttc aatgccagcg caagctcgcg ggtgtcgatg cccatcaacc gcgccttgtc
    13861 caggtctatg tcgagcgcga ggacccggtt tttttggtac cagtcgaatt ccaccatgct
    13921 gatcttttta ttgcggagca tgatctcgcg caacgcctcg gcggtttcct tgaccgtgtc
    13981 gtggccgtat cccgatacgc gaaacatcac cggatattgc gcaggcgggc cattggggag
    14041 gatcttgatg cgcgcgttca cgctgggaaa gctctgtgcg aatatcttct cgattttccg
    14101 agtgagccgc tcgcgggctt tgccgtcctt ggcgaggatc accagctgtc cgaagtttga
    14161 cgcgggcagg gtgggattga aggtgagcac gaaccgcggc gacccctggc cgacgtagct
    14221 tgcgaaattg acgatgtcgg ggtctttcc
//
LOCUS       DAORHF010000081        14036 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3408_length_14036_cov_5.166297, whole genome shotgun sequence.
ACCESSION   DAORHF010000081 DAORHF010000000
VERSION     DAORHF010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14036
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3408_length_14036_cov_5.166297"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3236
                     /locus_tag="PKJ16_08495"
     CDS             <1..3236
                     /locus_tag="PKJ16_08495"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47066.1"
                     /translation="GNRFSLETVSIVNGTSVEQSLEDLTAAAREGLIFFSAQEGRYRF
                     FHDRIGHLAIAESTTHDILMEKIFYIANHLNIARSLFESDGERFTLARMNLYAGMKAK
                     NAAAFESARGYFTQGTDVLPDSAFAEEYQLSFDLYLNRGECEYIAGRFDEATTIFAII
                     LTFARCALDRARIYNMLIILCTNGGEYREAVQFARDAMMMFGVDCSRENIRAAVSAEF
                     AKAMELLERLSLDEIAALPEMRDPELLARMEIMTNCAAATFYVDLDFNGFLPLHLFNC
                     SIEHGNSPYAAFGYSMFSVVHFVRGNFARAFAFGELALSLNRRYDNIATRSKVPVIVG
                     VYTVHWSRHVREGIALLEEGYRARHQTGDLIYTGFFAVHLALMRAQAGDELGPLLQDV
                     ERYLELTRQIRFDNAGLAIATIRQKILSLMGRTRDRAGMDSDGFSEQEHVARLRASEM
                     TFALHSHLVHAASLRYLFGDIRRAFEVSTEALRYSRYSTGLLHQAEENFFRSLIVAEL
                     VGAGMADIDVFSAELEENQRRLKLWAESSPGNFLHKYLLVEAERAILADDPWTAMARF
                     DEAIESALENGYVQHAALACERAGRFYRSRGRRRIARQYLIDADRFYGRWGAAAKRAH
                     LAEAYPGLLDRTPDEPSSGAPGDTDTGSDTISRDLDLSTIIKATHAVAREIDLGAILA
                     ALITIAIENAGAQSGYLLLENERDGRLYIEASGAVGERPDVLQSIPAASCDQLSAAVV
                     NYVHKTRENIVLDNALEQGMFTEDGCIARKRSRSLLCVPIMHKNRVIGVLYLENTLVA
                     GAFTPARVELLTILSSQAAISIENARLLATRESQARLAREMEIAARIQTILLPVAPRI
                     DGFEITGYMRPAEKVGGDYYDVINTPSRDWVIIGDVSGHGVSAGLVMMMVQTAIHFAL
                     SRRPGIRPVDLLTHINRGISENIHRISAEKYMTIVLLAHLGDGRFELAGLHPDILVYR
                     ARTGAVERVRLHGNWIGIPERVYPKNWKSRNQTLALHTGDVMLLYTDGITESRSADED
                     MLDQAGLERILQKAGGGSTNAVRDAILERLAEYTSTDDITMLILKRT"
     gene            3244..5487
                     /locus_tag="PKJ16_08500"
     CDS             3244..5487
                     /locus_tag="PKJ16_08500"
                     /inference="COORDINATES: protein motif:HMM:NF039873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV47067.1"
                     /translation="MRSVRLAAAIACSIGIALSARVSAGPGRADTPPPERRARIGLVL
                     SGGGAKGFAHIGALKVLEEAGVRVDYITGTSMGSVVGALYAIGYDAKALERLALDTDW
                     NDLLSDTVSRRYYSIDEKNEDGKYILSLPLHDGGIGLPRGLIAGQKTMLLLARLMARY
                     HHVRDFRQLPIPFRCVATDIENGKPIVLERGFLPEAVRASMSLPTAFNPVELDGRLLV
                     DGGVVRNFPVSDAREMGADIVIGIDTGAPLYSKKEIDSIAKIMEQSVSFLGDISTKEE
                     QKLCDILIQPAIREYNATSFSDAATLIARGEEAARRFLPQLAAIAADQRRHGIDPRPA
                     ASPALTGPIRISGITVTGLTNVSRSLLMGKLDIATPADITIDEIDEAMNRAYGSHFFE
                     QVYYRLEPGPDGRVLAVTVNENATSLLKLGIHYDTDIKAALLLNATFRNMLGQGSKLM
                     FDARLGQNPALIASYFIHSDFKPGIGFGVDVWFDQVDVDITDNQNNVIASLVYSDYGG
                     TMRFQTIFSNAFALGLAAEKQYTDIQKKIAHYDPGVTYFQFTNYFAFCKVDLLDRTTY
                     PRSGFALYGEAKLITDDLATKKDMNVSNYERYIARLDCFLPVHDRVSLLGACHWGLIR
                     PYQADTAPVPYPLLFYIGGLYTYQESLFPFYGYRFMEVMWREALVFQYGIQIEPRRDT
                     FVRLIANTAQTTSNAEEVPLQKDARLSGYGVSIGLLTPIGPIEITVMQSLQTHSVILH
                     FNLGYRF"
     gene            complement(5637..6209)
                     /locus_tag="PKJ16_08505"
     CDS             complement(5637..6209)
                     /locus_tag="PKJ16_08505"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV47068.1"
                     /translation="MSGRSFNIFKDIHVCPWWFSFFLDNLLRPRFHDPEKILSPYVKE
                     GYVAADIGCGPGYFSPTMARLVGESGRVVCIDLQRKMLDRARWNAERAGMANRMEFRQ
                     CSSDSLMLSEKLDFALTFWMVHEVPDSESFFRQIYDALKPGGRYLLVEPKMHVTRAKF
                     ERQCAIAGAVGFVPAGDPMVSLSRGMVFGK"
     gene            complement(6261..6602)
                     /locus_tag="PKJ16_08510"
     CDS             complement(6261..6602)
                     /locus_tag="PKJ16_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HNV47069.1"
                     /translation="MENVRHSGINKRRLSFYFMMASFLTLPVSGFLLHLYSRNPLAWQ
                     ARHACMAVHNLASMVFLAAVIVHLSLNAKSVMAYVRTRRDHIIPRAKELFIALSVMVV
                     LIALGVRHAFH"
     gene            complement(6674..7135)
                     /locus_tag="PKJ16_08515"
     CDS             complement(6674..7135)
                     /locus_tag="PKJ16_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV47070.1"
                     /translation="MDKRIESINGIMQQFLRVVNLFQEVERQPMDFGIGMMLHPSEIH
                     TVDAIGDSDGINVTALAHRLGVTKGAVSQMVTKLKRRGLVNKLKSVDNDRDVVLALTP
                     KGRRAYDAHKRFHLEMYHDFFTQIDDVTFEQIEFFKSIMDKIERYVERHLG"
     gene            complement(7199..8326)
                     /locus_tag="PKJ16_08520"
     CDS             complement(7199..8326)
                     /locus_tag="PKJ16_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47071.1"
                     /translation="MVSHGAQSGYAGSIRRIVRTCIVMGVGMILFASAVFAKGRILAV
                     GNQCMQYSDGSWRLVAEGPIFPSSVHFASPSDIFLAVSKDSPPRAGVKIPAGCGWARW
                     DPATDSIVPLPGTEKMKPRQIMPNKTVYYVEYRFHGDVLYEEKPGGKRTLLHSSRGMS
                     SFEYAPDGSHVYSIVPAMGGKESPYFSDWVIGSKTTLYLTRSKGKPKAIVTGDCIRDA
                     RLVANGAYAVFWEAGPVDMQGKTKAWRLRTVRLSDGAVTTVADVVDNLMGSRGVPEEF
                     WAFQGQPWIVHVASLKASKKGPREYVVYNVETGTRSSFTLAAGDRLYKYGGREYNMLL
                     FHPYLVAKRGDSLVVLTVPDLNTVLEAPLPTFYFDAAMYLP"
     gene            complement(8438..9259)
                     /locus_tag="PKJ16_08525"
     CDS             complement(8438..9259)
                     /locus_tag="PKJ16_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HNV47072.1"
                     /translation="MADYTFYLVEKKPPIAWVYLNRPEKKNAMNPPAWKESIPIFEDL
                     DRDNDIKVVILSGKGNAFTAGIDLMAMSAELSELGNREQLGGIKWNFIPKIYELQDTM
                     TCIEKCRKPVIAAVHGYCIGAGLDMITACDIRLCAQDAVFSLREAAVGFVADVGVLQR
                     LPHIVGQGITRELAFTAKNFDAKRAKEILLVNEIFPDHDALMKGAEALANEIVANSPL
                     AVQASKVVLNYGIGKTVDDALKYVASISSNIIPSEDLYEAITAFAQKRKPNFPGK"
     gene            9437..10441
                     /locus_tag="PKJ16_08530"
     CDS             9437..10441
                     /locus_tag="PKJ16_08530"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HNV47073.1"
                     /translation="MLQTKITDLFGIKHPIICGAMMWLSKPELCAAISNAGGMGNLTA
                     ANYNTEDEFRAAIRETRRLTDKPFMVGVTILPSIRITGEHHKMYLKVCAEEKVAGLEI
                     SGSPIDKSCGMEYIDMLKKAGVKLFHKVGSVRHAVHAEKVGYDGIYAAGIEEGGHPLN
                     DDVTTMVLTPRIAESVKIPVVTVGGIANGRSLAAALMLGAEGVMMASRFMATTDCPIH
                     DRVKQELVNRQEQDTTLICKSIHLQARALRNKTVEDILEVERTGGGLEGLLPLISGQR
                     MVKVWEEGDVEHAPMMVGQSIGLIHDVVSCTELLERMVAEAEERIRRCSSHMIAGVSD
                     "
     gene            10457..11044
                     /locus_tag="PKJ16_08535"
     CDS             10457..11044
                     /locus_tag="PKJ16_08535"
                     /inference="COORDINATES: protein motif:HMM:NF036525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47074.1"
                     /translation="MRKNNRDRILETARRLFLKYGFNGISIRDIAAGARLTTGAIYFH
                     FKNKRDIYAAICLEAVDLLIARFREAVVAGTTPPQKLISTYDAYLAFYYENRDYYNIL
                     MEYRAAYEPDDTDDIAAKMRELVDLMAQTIGLGQEKSYYRSVSPLMAAMFLASVAEGM
                     LQFKKIGMMESLRITDTEFRRCMDDIVWNGIKRES"
     gene            complement(11041..11874)
                     /locus_tag="PKJ16_08540"
     CDS             complement(11041..11874)
                     /locus_tag="PKJ16_08540"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV47075.1"
                     /translation="MMNASLLESLADDSRYSMVPRGEYAIGVDPDGAERIIPLLRDDR
                     MKPEWLTAATPRHAIALSGCRISNFLVTRGHFALFVDATGYRTEAERDGWGWVFEGGW
                     QKRGGVSWRSPFLDAGDDACRAHSPIVPVMQASWNDAEAFCRWLSEAIGRRFRMPTEG
                     EWEAFAGMHGAPSIVDCMAERVSIAPVNSREYVAMLIAAADADRSATPIGHVWEWCTD
                     WYDAYPGGPHHRDFGATYRVLRGGSLMSRPAQRGREYRFRRCPTARSPYYGVRLAMDI
                     A"
     gene            complement(11894..12979)
                     /gene="ispG"
                     /locus_tag="PKJ16_08545"
                     /gene_synonym="gcpE"
     CDS             complement(11894..12979)
                     /gene="ispG"
                     /locus_tag="PKJ16_08545"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HNV47076.1"
                     /translation="MTIRKKTRPVAVRSLVIGGEAPVSIQSMTNLPIEDVRGTVAQIE
                     RLRAAGADLVRLALRNEDSIAHLREIRTAVDMPLSADVHFNHRIAIKAIEAGIDKVRI
                     NPGNIGDDDRVRDVVAVARERRVPIRIGVNGGSIDLKTYHAVSPESLAQSALDHVRIL
                     EDNDFRDIVVSIKSSDIRQTIDANVRFSELRDYPLHIGLTEAGYGASCIVQSTLAIGH
                     LLLMGLGDTVRVSMTGDPVGEIPVARTILEAAGLRVPTVRIIACPTCGRTDPSLDILA
                     LAERVESETARFHDALYAAGRTVTVAVMGCEVNGPGEAAHADVGLAGGRGGRMILFAK
                     GEKIAVVDVGDAVARLVDEIEAMIAPA"
     gene            complement(12976..13599)
                     /locus_tag="PKJ16_08550"
     CDS             complement(12976..13599)
                     /locus_tag="PKJ16_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008063412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNV47077.1"
                     /translation="MKAYLRATDIIDWERPDVRSAARALAHNASDSIEIARRCFEFVR
                     DEVPHSWDHRINPVTLRASEALAHRTGFCYSKSHLLAALLRANGIPAGICYQRLEING
                     CPRPFALHALNAVHLDGFGWYRVDARGNRSDVDARFDPPNEWMAFFPTREGEMDLPEI
                     WADPLPVVVETLARYRDIEEARVNLPDCELWVTRARGAHCATGGINQ"
     gene            complement(13596..>14036)
                     /locus_tag="PKJ16_08555"
     CDS             complement(13596..>14036)
                     /locus_tag="PKJ16_08555"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HNV47078.1"
                     /translation="VHNAEEALMMRRADGPVPFFGGYPVSPDQFAVAIVVLTVIGFLV
                     TAAAWRFWGRRYFLLVMNGFLSMFILNAFFPHIIASIVTGTAAPGVATAVLLYLPFGV
                     YGVRSILRHDLLYRRHFIVSLAAGPVVAAALAGLCLLVGYLAVP"
BASE COUNT         2728 a         4635 c         4236 g         2437 t
ORIGIN      
        1 tcgggaaccg tttttccctc gagacggtgt cgatcgtcaa cgggaccagc gtggagcagt
       61 cgctcgagga cctcaccgcg gcggcgcgcg aggggctcat cttcttttcc gcgcaggagg
      121 gccgctaccg gttctttcac gaccgcatcg gccatctcgc gatagcggag tcgaccaccc
      181 acgacatcct catggaaaag atcttctata tcgcaaacca tctgaacatc gcgcgatcgc
      241 tcttcgagag cgacggggag cgcttcaccc tggcccgcat gaacctctac gccgggatga
      301 aggccaagaa cgcggccgcc ttcgaatcgg cgcgcgggta tttcacccag ggcaccgacg
      361 tgctgcccga ctcggccttc gccgaggagt accagctctc cttcgacctg tacctcaacc
      421 gcggggagtg cgagtacatc gccggccgtt tcgacgaggc gaccacgatc ttcgccatca
      481 tcctcacctt cgcgcgctgc gcgctggacc gggcccggat ctacaacatg ctcatcatcc
      541 tgtgcaccaa cggcggcgag taccgcgagg cggtccagtt cgcgcgcgac gcgatgatga
      601 tgttcggcgt ggactgctcg cgcgagaaca tccgcgcggc cgtctcggcc gaattcgcga
      661 aggccatgga gctcctggag cgcctctccc tcgacgagat cgccgcgctc cccgagatgc
      721 gcgaccccga gcttttggcg cgcatggaga tcatgaccaa ctgcgccgcc gccaccttct
      781 acgtggacct cgacttcaac ggctttctcc ccctgcacct gttcaattgc tcgatcgagc
      841 acggcaattc gccctatgcg gcgttcggct acagcatgtt cagcgtggtc cacttcgtcc
      901 gcggcaactt cgcgcgggcc ttcgccttcg gcgagctcgc gctctcgctc aatcggcgtt
      961 atgacaacat cgcgacccgc agcaaggtgc cggtgatcgt gggcgtgtac accgtccact
     1021 ggtcgcgcca cgtgcgcgag ggcatcgccc tcctggagga gggataccgc gcgcgccatc
     1081 agaccggaga cctcatctac acgggatttt tcgcggtgca cctcgcgctc atgcgcgcgc
     1141 aggccgggga cgagctcggc cccctgctcc aggacgttga gcgatatctc gagctcaccc
     1201 gccagatccg cttcgacaat gccgggctcg ccatcgctac gatccggcag aagatcctga
     1261 gcctcatggg gaggacgcgc gaccgcgcgg gcatggattc ggacgggttt tccgaacagg
     1321 aacacgtcgc gcgactgcgc gcaagcgaga tgaccttcgc gctccacagc catcttgtgc
     1381 acgccgcgtc cctgcgctac ctcttcggcg acatccgccg ggccttcgaa gtgtccacgg
     1441 aggcgctgcg ctactcccgc tactcgacgg gcctgctcca ccaggccgag gagaactttt
     1501 tccgctcgct tatcgtcgcg gagctcgtgg gcgcggggat ggccgacatc gacgttttta
     1561 gcgccgagct cgaggagaac cagcgccgcc tgaagctgtg ggccgagagc agtcctggga
     1621 attttctcca caagtatctg ctcgtcgagg ccgagcgggc gatcctggcc gacgatccct
     1681 ggaccgcgat ggcgcgcttc gacgaggcca tcgagtcggc gctggaaaac gggtacgtgc
     1741 agcatgcggc gctcgcctgc gagcgggccg gccggttcta tcgctcccgc gggcggcgac
     1801 ggatcgcgcg ccagtacctt atcgacgccg accgcttcta cggtcgctgg ggggcggccg
     1861 cgaaacgcgc gcaccttgcg gaggcgtatc ccggccttct tgaccggaca ccggacgaac
     1921 cgtcgtcggg cgcacccgga gacaccgaca ccggaagcga tacgatctcg cgcgacctgg
     1981 acctctcgac catcattaaa gccacccacg cggtcgcgcg ggagattgac ctgggcgcca
     2041 tcctcgccgc cttgatcacc atcgccatcg aaaacgccgg agcgcaatcc ggctacctgc
     2101 tcctcgaaaa cgaacgcgac ggccggctgt acatcgaggc atccggcgcc gtgggcgagc
     2161 gccccgacgt gctgcaatcg atcccggcgg catcctgcga tcagctctcg gcggccgtcg
     2221 tcaactacgt ccacaagacg cgggagaaca tcgtactgga caacgccctc gagcagggca
     2281 tgttcaccga ggacggctgc atcgcgcgca agcgatcgcg atcgctcctg tgcgtcccca
     2341 tcatgcacaa aaaccgggtg atcggcgttc tttatctcga aaacactctc gtcgccggcg
     2401 cctttacgcc cgcgcgcgtc gagctgctca ccatcctctc gtcgcaggcg gccatctcca
     2461 tcgagaacgc ccggctcctg gccacccgcg agagccaggc gcggctggcg cgcgagatgg
     2521 agatcgcggc gcgcatccag accatcctgc tgccggtcgc gccgcgcatc gacggcttcg
     2581 agatcaccgg ctacatgcgg ccggccgaaa aggtcggcgg ggactactac gacgtcatca
     2641 acacaccgtc gcgggactgg gtgatcatcg gggacgtgtc ggggcacggc gtttcggccg
     2701 ggctggtgat gatgatggtg cagaccgcga tccacttcgc cctctcccgg cggccgggca
     2761 ttcgtccggt ggacctcctc acgcacatca atcgcgggat atcggagaac atccaccgca
     2821 tctcggcgga gaagtacatg accatcgtgc tcctggcgca cctcggcgac ggccgcttcg
     2881 agctggcggg actgcaccca gacatcctgg tgtatcgcgc gcggaccggg gcggtggagc
     2941 gggtgcgcct gcacggcaac tggatcggca tccccgagcg cgtgtacccg aaaaactgga
     3001 aatcgcggaa ccagacgctc gcgctgcaca cgggcgacgt catgctcctc tacaccgacg
     3061 gcatcaccga gtcgcgcagc gcggacgagg acatgctcga ccaggcggga ttggagcgga
     3121 tcctccagaa ggccggcggg ggatcgacca acgcggtccg agacgcgatc ctcgagcgcc
     3181 tcgccgagta caccagcacc gacgacatca ccatgctgat cctgaagcgc acctgacgag
     3241 gccatgcgct ccgtccgact cgccgccgcc atcgcctgtt cgatcggtat cgcgctttcg
     3301 gcgcgcgtgt ccgcagggcc cggtcgcgcc gacacgcctc ccccagagcg gcgggcgcgc
     3361 atcgggctcg tgctcagcgg cggcggcgcc aaggggttcg cccatatcgg cgcgctcaag
     3421 gtgctcgagg aggcgggggt gcgcgtggac tacatcaccg gcacgagcat gggcagcgtg
     3481 gtgggcgcgc tctacgccat cggttacgac gcgaaggcgc tcgagcggct ggcgctggac
     3541 accgactgga acgacctgct gagcgacacg gtctcgcgcc gctactactc gatcgacgag
     3601 aaaaacgagg acgggaaata catcctgagc ctcccgctgc acgacggggg gatcggactg
     3661 ccccgggggc tcatcgccgg ccagaagacc atgctgctgc tggcgcgcct gatggcgcgc
     3721 tatcaccacg tccgcgactt ccgccagctc cccatcccct tccgctgcgt ggccaccgac
     3781 atcgaaaacg ggaagccgat cgtgctcgag cgcggcttcc tgccggaggc cgtccgcgcg
     3841 agcatgtcgc tgcccacggc cttcaacccg gtcgagctcg acgggcggct gctcgtggac
     3901 ggcggcgttg tgcgaaattt cccggtatcc gacgcgcgcg agatgggcgc cgacatcgtg
     3961 atcgggatcg acaccggcgc gccgctgtac tccaagaagg agatcgactc catcgccaag
     4021 atcatggagc agtcggtgag ctttttgggc gatatctcga cgaaggagga gcagaagctc
     4081 tgcgacatcc tgatccagcc ggccatccgc gagtacaacg ccaccagctt ctccgacgcg
     4141 gccacgctca tcgcgcgcgg cgaggaggcg gcgcgccggt ttttgccgca gctcgcggcc
     4201 atcgccgccg accagcggcg ccatggcatc gacccgcgcc ctgccgcgtc gccggcgctc
     4261 acggggccga tccgcatctc cggcatcacc gtcaccggcc tcacgaacgt ctcgcgctcg
     4321 cttctcatgg gaaagctgga catcgcgacg ccggccgaca tcaccatcga cgagatcgac
     4381 gaggcgatga accgcgccta cggcagccac ttcttcgagc aggtgtacta tcggctcgaa
     4441 ccggggccgg acgggcgggt gctggccgtc acggtgaacg agaacgcgac atcgctgctc
     4501 aagctgggaa tccattacga caccgacatc aaggcggcgc tgttgctcaa tgcgaccttc
     4561 cgcaacatgc tcgggcaggg ctcgaagctg atgttcgacg cgcgcctggg ccagaacccc
     4621 gccctcatcg cctcctactt catccattcc gacttcaagc cggggatcgg gtttggcgtg
     4681 gacgtatggt tcgaccaggt cgatgtggac atcaccgaca accaaaataa cgtcatagct
     4741 tcgctggtgt actccgacta cggcgggacc atgcggttcc agaccatctt ctcgaacgcc
     4801 ttcgccctcg gcctcgccgc cgagaagcag tacaccgaca tccagaagaa gatcgcccac
     4861 tatgatccgg gcgtcaccta cttccaattc accaactatt tcgcgttttg caaggtggac
     4921 ctgctcgatc gaaccaccta cccgcgcagc ggattcgccc tgtacggcga ggcgaagctc
     4981 atcaccgacg acctcgcgac gaaaaaggac atgaacgttt cgaactacga gcgctacatc
     5041 gcgcgcctcg actgcttcct cccggtgcac gaccgggtct cgctgctggg cgcctgccac
     5101 tggggtctca tccggccgta tcaggcggac accgcgccgg tcccctatcc gctgcttttt
     5161 tacatcggcg gcctgtacac ttaccaggaa tctctctttc cattctatgg ataccgtttc
     5221 atggaggtga tgtggcgcga ggccctcgtc ttccagtacg gcattcagat agagccacgg
     5281 cgcgacacct tcgtgcggct gatcgcgaac accgcccaga ccacctcgaa tgccgaggag
     5341 gtcccccttc agaaggacgc gcgcctgtcg ggctacggcg tttcgatcgg gctgctcacg
     5401 cccatcggcc cgatcgagat cacggtgatg cagtcgctac agacgcacag cgtgatcctg
     5461 cactttaacc tggggtaccg tttttaacac cgacgcgttt ttgttgagta ttggccatgc
     5521 tctgaccccc gccaagccag acgctgaagg cctccgaagg ctctgacccc cactttggca
     5581 tggcagtgtg gggctctgac cccgtttttc acaggaaatc gtgggggctc tgacccctac
     5641 ttcccgaaaa ccatcccccg gctcaaggaa accatcgggt cgccggcggg cacgaaaccg
     5701 accgccccag caatcgcgca ctgccgctcg aacttcgctc gcgtcacatg catctttggc
     5761 tcaacgagga gataccgccc gcccggcttc agtgcgtcat agatctgccg gaagaaggat
     5821 tccgagtccg gcacctcatg caccatccag aaagtgagcg cgaagtcgag cttctcggag
     5881 agcatcagcg aatcggacga acactgccgg aactccatcc gatttgccat tcccgcgcgc
     5941 tcggcgttcc accgcgcccg gtcgagcatc ttccgctgta gatcgatgca gacgaccctg
     6001 ccgctttcac cgacgagccg ggccatcgtt ggcgagaagt atcccggccc gcaaccgata
     6061 tcggccgcga catatccttc cttcacgtac ggtgagagga tcttttccgg atcgtgaaat
     6121 cgaggccgaa gcaggttgtc gagaaaaaac gaaaaccacc aggggcatac atggatatcc
     6181 ttgaaaatgt taaaactccg tccgctcaca tcaacctccc tttatttgac acatgtattg
     6241 caacacgaca cattgaacga tcaatggaac gcatgccgga cacccagagc gatcaaaaca
     6301 accatcaccg aaagagcgat gaagagttcc tttgctcgag gaatgatgtg atccctcctg
     6361 gtccgcacgt acgccatcac gcttttcgca ttgagcgata gatgaacaat gacggcggca
     6421 agaaatacca tcgatgccag attgtgaacc gccatgcagg cgtgccgcgc ctgccaagcc
     6481 agcgggttcc gggagtacag gtgcaacaga aacccggaga ccggcaaagt cagaaatgac
     6541 gccatcatga aataaaacga caaccttcgt ttgttgatac cgctgtgccg tacgttctcc
     6601 atttctcctc cgaaagcgct accacgttgg gcatattcta atggtttaga tgctaaactt
     6661 tttgcgaaaa aaatcacccg agatgccgct ccacatatcg ctcgatcttg tccatgatcg
     6721 atttgaaaaa ttcgatctgt tcaaacgtga catcatctat ctgcgtaaaa aaatcatgat
     6781 acatctccag gtgaaagcgt ttgtgcgcat cgtaggcacg cctccctttc ggcgtgagcg
     6841 caaggacaac atcacggtca ttgtccacac ttttcagctt gttcaccaac ccccgccttt
     6901 tcagtttcgt gaccatctga gagacggcgc ccttggtcac accgagccgg tgcgcaagcg
     6961 ccgtcacgtt gatgccatca ctgtcaccaa tggcatcgac ggtatgaatt tccgacggat
     7021 ggagcatcat gccgattccg aaatccatcg gttgccgttc cacctcctga aacaggttta
     7081 ccacccgcag gaactgctgc attatgccgt tgatggattc gatgcgtttg tccatatcca
     7141 taatgtatag cagctaaaca atatcgtcaa cacaaaattc cgctggcgcg aaaaaaaatc
     7201 acggcagata catcgccgca tcaaaataga acgtgggtaa tggcgcctca agcaccgtgt
     7261 tcaggtcggg gaccgtgagc acgaccaggg aatcaccccg cttcgctaca agatacggat
     7321 gaaacaggag catgttgtat tcccgaccgc cgtacttata tagccgatcg cccgccgcga
     7381 gcgtgaagga cgaacgcgtt cccgtttcga cgttatacac gacgtactcg cgcggtccct
     7441 tcttcgaggc tttgagggaa gcgacatgca cgatccacgg ctggccctgg aaggcccaga
     7501 attcctcggg aacgccgcgc gagcccatga gattgtcgac gacatcggcg acggtcgtca
     7561 ccgcaccgtc ggacaggcgc accgtcctga gccgccaggc cttggtctta ccctgcatgt
     7621 caaccggccc ggcctcccag aacacggcat aggcgccgtt cgccaccagt cgggcgtccc
     7681 ggatgcaatc ccccgtcacg atcgctttcg gcttcccctt cgagcgggta agatagagcg
     7741 tcgtcttcga gccgatcacc cagtccgaga aatacggcga ttcctttccg cccatggccg
     7801 ggacgatcga atacacatgg ctgccgtcgg gcgcgtactc gaatgagctc atgccgcgcg
     7861 atgaatggag gagggtgcgc ttcccgcccg gtttttcctc gtacagcacg tcaccgtgga
     7921 aacgatactc cacgtagtag acggtcttgt tcggcatgat ctggcgcggt ttcatcttct
     7981 cggttccggg gagggggaca atgctgtcgg tcgccggatc ccagcgcgcc catccgcagc
     8041 cggcgggtat tttcactccc gcgcgcggcg gcgaatcctt cgacaccgcc aggaagatat
     8101 cagacggact cgcgaaatgc accgagctcg ggaagatggg accctcggcc accaggcgcc
     8161 atgagccatc ggaatactgc atacactggt tcccgacggc aaggatcctc cccttggcga
     8221 acaccgccga tgcgaaaagg atcatcccca cgcccataac gatgcaggtc cggacgattc
     8281 tccgtatcga gcccgcatac cccgactgcg caccatgaga aaccatagcc cctcctctga
     8341 aaacaagaat ccccggcaat gagcgccggg gatagggtat gtcgcgtttc agaaaaaagc
     8401 aacagaaaaa gcgcatgatt gcgcttcgcg ccgtgcctca cttcccgggg aagttcggct
     8461 tgcgcttctg cgcgaaggcg gtgatcgcct cgtagaggtc ctccgacggg atgatattgg
     8521 agctgatgga ggcgacgtac ttgagcgcgt cgtcgacggt ctttccgatg ccgtagttga
     8581 ggaccacctt cgacgcctgg accgcgagcg gcgagttggc gacgatctcg ttcgccaacg
     8641 cctcggcgcc cttcatgagc gcatcgtggt cgggaaagat ctcgttcacc agcaggatct
     8701 ccttcgcgcg tttcgcgtcg aagttcttcg cggtgaaggc gagctcgcgc gtgatcccct
     8761 gccccacgat gtgcgggagg cgctgaagga cgcccacgtc ggccacgaag cccacggccg
     8821 cctcacgcag cgagaagacc gcgtcctgcg cgcacaagcg gatgtcgcag gcggtgatca
     8881 tatcgaggcc cgcgccgatg cagtagccgt gcaccgcggc gatgacgggc ttgcggcact
     8941 tctcgatgca ggtcatcgtg tcctgcagct cgtaaatctt cggaatgaag ttccacttga
     9001 tgccgcccag ctgctcgcgg ttccccagct ccgagagctc ggccgacatg gccatgaggt
     9061 cgatacccgc ggtgaaggcg tttcccttcc ctgagaggat caccaccttg atgtcgttgt
     9121 cgcggtccag gtcctcgaag atcggaatgg actctttcca ggcgggcggg ttcatcgcgt
     9181 tctttttctc cggcctgttc agatacaccc aggcgatcgg cggtttcttt tcaacgaggt
     9241 agaatgtgta gtcggccata gttctgctcc tttgctggat tccgtggccc atcttcccgg
     9301 ccgaatagtt tatttcaaga aaaaaataac actgttatgt aggtgcagtg tgatgagggc
     9361 accgctttga aaaaatgtgt tgacaataac ataacactgt tatgttattg cgccatcttt
     9421 tccgaggagc gaaaacatgc tacagactaa aatcaccgac ctgttcggca tcaaacatcc
     9481 catcatctgc ggggccatga tgtggctctc gaaaccagag ctgtgcgccg cgatatcgaa
     9541 cgcgggcggc atggggaacc tcaccgccgc gaactacaat accgaagacg agttccgcgc
     9601 tgcgatccgc gagacgcgcc gtctcaccga caagccgttc atggtgggcg tcaccatcct
     9661 gccctccatc cgcatcaccg gcgagcacca caagatgtat ctcaaggtct gcgccgagga
     9721 gaaggtcgcc ggcctggaga tctccggcag ccccatcgac aaatcgtgcg gcatggaata
     9781 tatcgacatg ctcaagaagg ccggcgtcaa gctctttcac aaggtgggct cggtgcgcca
     9841 tgccgtgcac gccgagaagg tcggctacga cggcatctac gccgccggca tcgaggaggg
     9901 cggccatccg ctgaacgacg acgtgaccac gatggtgctc acgccccgca tcgccgaatc
     9961 ggtgaagatc ccggtcgtga cggtgggcgg catcgcgaac gggcgatcgc ttgccgccgc
    10021 gctcatgctg ggcgccgagg gggtcatgat ggccagccgc ttcatggcca ccaccgactg
    10081 ccccatccac gaccgcgtga agcaggagct ggtgaaccgc caggaacagg acaccaccct
    10141 catctgcaag agcatccacc tgcaggcgcg ggcgctgcgc aacaagaccg tcgaggacat
    10201 cctcgaggtg gagcggacgg gcggcgggct tgagggcctc ctcccgctca tctcgggcca
    10261 gcgcatggtc aaggtctggg aggaaggcga cgtggagcac gcgcccatga tggtgggcca
    10321 gtcgatcggc ctcatccacg acgtcgtctc gtgcaccgag ctgctcgagc gcatggtggc
    10381 cgaggccgag gagcggatca ggcgctgctc gtcgcacatg atcgcgggcg tgtcggacta
    10441 ggcggacggc cgcacgatgc ggaagaacaa ccgcgaccgg atcctcgaaa cggcccgtcg
    10501 cctgttcctg aaatacgggt tcaacggaat ctccatcagg gacatcgcgg cgggcgcgcg
    10561 cctcacgacg ggcgcgatct actttcattt caagaacaaa cgcgacatct acgccgcgat
    10621 ctgcctggag gcggtggacc ttctcatcgc gcgctttcgc gaggccgtcg tcgccgggac
    10681 caccccgccg cagaagctca tctccacgta cgacgcctac ctcgccttct actacgagaa
    10741 ccgcgactat tacaacattc tcatggaata ccgcgcggcg tacgagcccg acgacacgga
    10801 cgatatcgcg gcgaagatgc gggagctcgt ggacctcatg gcccagacga tcgggctcgg
    10861 gcaggagaag agctactacc ggtccgtgag cccgctcatg gccgcgatgt tcctcgcctc
    10921 cgtcgcggag ggcatgctcc agttcaagaa gatcggcatg atggagagcc tgcgcatcac
    10981 cgacaccgaa ttccgccgct gcatggacga catcgtctgg aacggcatca agcgggaaag
    11041 ttaggcgata tccatcgcga gccgcacccc gtaatacggg ctccgagccg tggggcagcg
    11101 ccggaaccgg tactccctcc ctcgctgcgc cgggcggctc ataagcgagc cgccgcgaag
    11161 cacccgatag gtggccccga aatcgcgatg gtgcggcccg cccggatagg cgtcgtacca
    11221 atcggtgcac cactcccaca catggccgat cggcgtggcg ctccgatcgg catcggcggc
    11281 cgcgatcagc attgccacgt attcgcgcga attcacgggg gcgatgctca cgcgctccgc
    11341 catgcaatcg acgatcgagg gcgcgccatg catgccggcg aacgcctccc attcgccctc
    11401 cgtcggcatc cggaatctcc gcccgatcgc ttcggagagc cagcggcaga acgcctcggc
    11461 gtcgttccag ctcgcctgca tcaccgggac gatcggcgaa tgcgcgcggc acgcgtcatc
    11521 accggcatcg aggaagggag agcgccatga gacgccgcca cgcttttgcc agccgccctc
    11581 aaagacccac ccccagccgt cgcgttccgc ttccgtccgg tagccggtgg cgtcgacgaa
    11641 gagcgcaaag tgcccgcgcg ttaccaggaa attcgagatg cggcagccgg acagggcgat
    11701 cgcatggcgg ggggtcgccg cagtgagcca ttccggcttc atgcggtcgt cgcgcagcag
    11761 tgggatgatc cgctccgcgc cgtccggatc gacgccgatt gcgtactccc cgcgagggac
    11821 catcgagtac cgcgaatcat cggccaggga ttcaaggagg gacgcgttca tcatgccgat
    11881 ctgattgcgg ccgtcacgcc ggtgcgatca tcgcctcgat ctcgtcgacg agacgcgcaa
    11941 cggcatcgcc cacatcgacg acggcgatct tctcgccctt tgcgaacagg atcatgcggc
    12001 cgccccgccc tcccgcaagg cccacgtcgg catgcgccgc ctcgccggga ccgttcacct
    12061 cgcagcccat caccgcaacc gtgacggtcc gacccgcggc atagagcgca tcatggaacc
    12121 gcgcggtctc cgactccacc cgctcggcca gcgccaggat gtcgagggac ggatcggtgc
    12181 gcccgcaggt gggacacgcg atgatgcgca ccgtcgggac gcgcaggccc gcggcctcga
    12241 gtatcgttcg cgcgacgggg atttcgccga cgggatcgcc ggtcatcgag acgcggacgg
    12301 tgtcgcccag tcccatgagc agcagatggc cgatcgcgag cgtgctctgc acgatgcacg
    12361 aggcgccgta gcccgcctcg gtgagcccga tgtggagcgg atagtcgcgg agctcggaaa
    12421 accgcacgtt cgcgtcgatc gtctgccgga tgtccgagga cttgatggac acgacgatgt
    12481 cgcggaaatc gttatcctcg agtatccgca catggtccag cgccgattgc gcgaggctct
    12541 cggggcttac ggcatgatac gttttcaggt cgatcgagcc gccgttcacg ccgatgcgaa
    12601 tggggacccg ccgttcgcgc gccaccgcga ccacatcgcg cacgcgatca tcatccccga
    12661 tattgccggg attgatgcgc accttgtcga tccccgcctc gatggccttg atcgcgatgc
    12721 ggtggttgaa gtgcacgtcg gccgagagcg gcatgtccac ggccgtacgg atctcccgca
    12781 ggtgcgcgat cgaatcctcg tttcgcaggg ccaggcgcac gagatcggcg ccggcggcgc
    12841 ggagccgctc gatctgcgcg acggtcccgc gcacgtcctc gatggggaga ttggtcatcg
    12901 actggatgga aaccggcgcc tccccgccga tgaccagcga gcgcacggca acaggacgag
    12961 tcttctttcg tattgtcatt ggttgatccc gccggtcgcg cagtgcgcgc cacgagcgcg
    13021 agtcacccac agctcgcagt cgggaagatt gacgcgcgcc tcttcgatat cgcgatatcg
    13081 tgcgagcgtc tcgacgacga ccgggagcgg atcggcccag atctccggca ggtccatttc
    13141 gccctcccgt gtcggaaaaa aagccatcca ttcattgggc ggatcgaagc gagcgtcgac
    13201 gtcggagcgg ttgccgcgcg cgtccacgcg gtaccagccg aacccatcga gatggacggc
    13261 gttgagcgca tggagcgcga acgggcgcgg gcatccgttg atttccagac gctgatagca
    13321 gatgcccgcg ggaatcccgt tggcccgcag gagcgccgcc aggagatggc tcttcgaata
    13381 gcaaaagccg gtccggtgcg ccagggcctc ggacgcgcgg agcgtcacgg ggttgatccg
    13441 gtggtcccaa ctgtgcggga cctcgtcacg gacgaactcg aagcagcgcc gcgcaatctc
    13501 gattgaatcc gatgcgttat gcgcaagggc acgcgccgcg gagcgcacgt ccggacgctc
    13561 ccagtcgatg atatcggtcg cgcgaagata ggccttcatg ggacagcgag ataaccgacg
    13621 agcaggcaga gcccggcaag ggccgcggcc acgacggggc cggcggccag ggaaacgatg
    13681 aagtggcggc gatacaggag atcgtgcctc aggatggaac gcactccata gacgccaaac
    13741 ggcagataca gcagcacggc ggtcgccacg cccggcgccg ccgtgccggt aacgattgat
    13801 gcgatgatgt gcgggaaaaa tgcgttcagg ataaacatgg aaagaaagcc gttcatcacg
    13861 agcaggaagt agcggcgccc ccagaaccgc cacgcggcgg ccgtgacgag aaatccgatt
    13921 accgtgagga cgacaatcgc gacggcgaac tggtcgggcg acaccggata gcccccgaaa
    13981 aacgggaccg gtccgtccgc gcggcgcatc atcagcgcct cctcggcgtt atggac
//
LOCUS       DAORHF010000082        13945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3444_length_13945_cov_4.702232, whole genome shotgun sequence.
ACCESSION   DAORHF010000082 DAORHF010000000
VERSION     DAORHF010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13945
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3444_length_13945_cov_4.702232"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /gene="arsB"
                     /locus_tag="PKJ16_08560"
     CDS             complement(<1..1018)
                     /gene="arsB"
                     /locus_tag="PKJ16_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HNV47079.1"
                     /translation="MSEAISKRLSFLDRYLTVWIFLAMAVGIVIGFIFQGPVERFNKA
                     LTVGEHTNMLIAVGLILMMYPPLAKVKYELMPTVFADVKILGLSLVQNWIVGPVLMTA
                     LSIAFFGFIAPAVFGPDPRWELFMTGLILVGIARCIAMVLVWNQLAGGNSEYGAGLVA
                     LNSIFQILTFSIYAWAFITILPPLFGLQGTAVNVTIGDIFVSVMIYLGIPFAAGVLSR
                     VILVPVKGHEWYDTVFIPKISPMTLVALLFTIVVMFSTQGKQIINRPLDVLWIAIPLS
                     CYFVIMFMASFFMARRLGADYSKSTTLAFTGAGNNFELAIAVAIAVFGIASPIAFATV
                     VGPLV"
     gene            complement(1055..1468)
                     /locus_tag="PKJ16_08565"
     CDS             complement(1055..1468)
                     /locus_tag="PKJ16_08565"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HNV47080.1"
                     /translation="MKNMIIAVLIGFTVFTPASHFSLSAAPTVKTVVYYFHGQFRCYS
                     CTRIEELASIALQKHFSKELASGAVVWRPVNVDAPGNERYIADYRLRTRSVVASRVID
                     GKEISWKNLERVWHYYNDEPAFTAYIKSEIDTLMC"
     gene            complement(1490..2731)
                     /locus_tag="PKJ16_08570"
     CDS             complement(1490..2731)
                     /locus_tag="PKJ16_08570"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HNV47081.1"
                     /translation="MKRAHRVLLVLLVVFALAGMALSFALIQEFYGVASGAVKSVCTS
                     SHTGVNSCRTVAESAYSALRGIPIIGEIPVAAAGFAFYGFMAALFLRSLFSDDVIRLR
                     GSFALGAVLSLTAVAGNLVLLYLSVAVIRAVCPLCSYTYIANAILLMLSLAGWFSARE
                     RGGDGIAATAIASVRTGLVPYAVTALVLLTAGIASAQLSKRAFDARLSESATDEQVRA
                     LISLYRSTAPVAIDLDGSPRLGPANATVRIVIYMDFTCDHCRDAGNTLSDLRERWPDK
                     ITIIYKNYPLDGPCTKLDRGRDDNSAGACIAAVASLCSWRLGKYPDVYRALYDNSERG
                     VKHTQTSVREIARRCGLAQATFESCIQSKETADIVARDVAEGDRLGIDGTPRVYINGR
                     LVPNDHLGAPVLSGLMNYLTN"
     gene            complement(2740..3453)
                     /locus_tag="PKJ16_08575"
     CDS             complement(2740..3453)
                     /locus_tag="PKJ16_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNV47082.1"
                     /translation="MVLELFTWLSGTLTNSAGIALAGAFLWGVLSIVLSPCHLASIPL
                     IVGFIDEQGTITTKRAFTLALLFSLGILVTIGIIGLITGLMGRMMGDIGAWGNYFVAV
                     IFFIVGLHLLGVIPLPFLEKGANPSFQKKGLLAALALGLIFGVALGPCTFAYMAPMLG
                     VVFSVAATQLVFAVSLIMAYAIGHCAVIVLAGTFTEVVQKYLNWNESSKGALILKKAC
                     GVLVIAGGIYLVVSQLMIG"
     gene            complement(3453..3971)
                     /locus_tag="PKJ16_08580"
     CDS             complement(3453..3971)
                     /locus_tag="PKJ16_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV47083.1"
                     /translation="MKRILKVTIPALAAIAIVTALPHLKSGDGCSCQTPLIVFGSLFG
                     CSDTKQPENTPVPIASNDVAAVPAGMPEKKAGPVEHKVTFVEIGSVNCIPCRMMQPIM
                     KAVEEEYKGQVKVVFHDVWTPQGKRDAMKYGIRVIPTQIFLDKNGVEYFRHEGFYPKE
                     QVVRALKMKGVN"
     gene            complement(4033..4194)
                     /locus_tag="PKJ16_08585"
     CDS             complement(4033..4194)
                     /locus_tag="PKJ16_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47084.1"
                     /translation="MADALINIKAQDIMRMKAIVIDVDRDEAIELIREFLRRAETQLQ
                     GGLKNHLDA"
     gene            complement(4631..4861)
                     /locus_tag="PKJ16_08590"
     CDS             complement(4631..4861)
                     /locus_tag="PKJ16_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013037272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV47085.1"
                     /translation="MKLEILGTGCAKCIKLEELTRKAVADLGVDAEILKVKDIKDIMS
                     YGVMMTPALVVDGEVKVVGKVPSVEEIKKFIL"
     gene            complement(4980..5438)
                     /locus_tag="PKJ16_08595"
     CDS             complement(4980..5438)
                     /locus_tag="PKJ16_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HNV47086.1"
                     /translation="MKRSLFIAILALMTAAFVTDSVFAQSGNYRRYQSSGRNYDPATV
                     ETVKGAVSLVETFTGGRRGGGLHLTITTSAGTVRIALGPASFIGGKMSFAKGDAVEVT
                     GSSVKNAQGEKLIIAKVVKKGSTELVLRDAAGNPLWSGQGSRGRGRGRNR"
     gene            complement(5697..5927)
                     /locus_tag="PKJ16_08600"
     CDS             complement(5697..5927)
                     /locus_tag="PKJ16_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HNV47087.1"
                     /translation="MKRARAITTANPLATVCVAALAVIFSTAFFFSRPSIEFEKLEYD
                     FGEVPEKTEQSCEFVFRNKGSGTLKIERVSAG"
     gene            complement(5943..7247)
                     /locus_tag="PKJ16_08605"
     CDS             complement(5943..7247)
                     /locus_tag="PKJ16_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HNV47088.1"
                     /translation="MKVDWKIEWKALLFIGGFFLALFYMPVGTRHFDNAVFEALYLAK
                     WYAQEHVLLCLVPAFFIAGAIGVFVRQESVMKYLGPTANKVLAYGVGSVSGSILAVCS
                     CTVLPLFSGIYRMGAGLGPAIAFLYSGPAINVLAIILTARVLGIEIGIARAVGAVAFS
                     VVIGAIMHFIYRKEELEKVNAAMQLPQTETKRPLWKDIVYFASMVAILVFANWGKPAE
                     TSGFWYAMFAYKWYITAASAIVFAAALVLWFDFKLWKVVAASVPVAVLAFFFSHEPTI
                     PFAAGAIGLAIIASTDNGEGGEWFSSTWDFAKQITPLLLGGVLVSGFLLGRPGNEGII
                     PSAWVSAAVGGNSLAANFFAAVVGAFMYFATLTEVPIVQGLIGSGMGAGPALALLLAG
                     PALSLPNMLVIRNYIGTQKTIVFCSLVIIMATISGYIFGMFF"
     gene            complement(7317..7655)
                     /locus_tag="PKJ16_08610"
     CDS             complement(7317..7655)
                     /locus_tag="PKJ16_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV47089.1"
                     /translation="MNDATRKQYVARASIIKAMAHPTRLFVIEALEKGERCVNDLTDM
                     IGADMSTVSKHLSILKNAGIIGDKKSGTSVFYHLKTPCILGFFGCIEDVIESNMRQQG
                     EVLKSCKARR"
     gene            complement(7686..7907)
                     /locus_tag="PKJ16_08615"
     CDS             complement(7686..7907)
                     /locus_tag="PKJ16_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47090.1"
                     /translation="MFKPLLEPGDVDFFNKLERLVMVIVDSDSDRPLNVKRVKLDNRT
                     LDFHKPPPAAVKCFSSIPIKYIDEYFLFG"
     gene            8110..8832
                     /locus_tag="PKJ16_08620"
     CDS             8110..8832
                     /locus_tag="PKJ16_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV47091.1"
                     /translation="MDLISARDITKIYRIGEVEITAIKNLSFTISPASFVSFVGPSGS
                     GKTTLLNLIGCLDMPTEGTMEIVGVKTPALGWKERATFRGEHIGFIFQDFNLLPVLTV
                     YENIEYPLIMVQNVEPAERKKRVLRLLDEVGMADQKGKYPDQISGGQKQRVAIARALS
                     TNPKLVLADEPTANLDHATAYMVLGLMKKMRDEFNTTFIFSTHDPKIVGEAEIIYTLE
                     DGALKSKQVVKKGKAARGRKHA"
     gene            8825..10063
                     /locus_tag="PKJ16_08625"
     CDS             8825..10063
                     /locus_tag="PKJ16_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47092.1"
                     /translation="MHNIIKIAIRNLVRYRRRTLLTASLITIGVVFVIVFIAVSGSFK
                     NMMIGQITDSMLGHIQIHKKGYVSSIDNLPLNLALKEAAVKEIGAALDGMPNVEAYSV
                     RIKFGGMFSNFAETTNIRLNAVYPEKEFKTVPLLIDRIIDGKKGLQRGEIFIPELLAK
                     GMNVKPGDTAVIIATNRDGSVNGKQFIVTGVLGSLSGPGGRDGYIHYEDGVELLRMDE
                     PEASEIAVRLKDFGALYKAEAMMKKLLVKNANGDIAGLLEVHTWETLSPFFNIAKMID
                     VMTVFIRIMLVAIVLVSIMNVMIMAVYERIREIGTIAAIGTMPGKILSMFMVEGLCLG
                     AAGSVLGGIIGVIVVFAINIAKVKFNFGMQTGLVLKASVNPSDLFAISIIVIIISLIA
                     SLQPAFKASRMEPITALRHV"
     gene            10124..10828
                     /locus_tag="PKJ16_08630"
     CDS             10124..10828
                     /locus_tag="PKJ16_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012729460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV47093.1"
                     /translation="MMISPAATYAGDNERLLKEIDRNLNPQSYESYRKLINIEPGGRK
                     KEFVMFSVKKGLEKMVVLFISPASEKGRSTLRLGENMWLYIPNVGKPVRITSLQSVVG
                     GVFNNADILQLDYAAEYNVETVESKGQQYLLHLKAKTKTVAYDRIRMWADKERKLPVK
                     IECLTEKNMLIKTLHFKTIKDFGGGIVRPSVIETDSPLYKNYKSIMVFAKLRKRVVSD
                     EVFTMTFMPRVDSLRK"
     gene            10825..12132
                     /locus_tag="PKJ16_08635"
     CDS             10825..12132
                     /locus_tag="PKJ16_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47094.1"
                     /translation="MKRLFPVLLLLVATVTAAYGEDEYAFDERELGAETEKKPYHVGG
                     FAELRPVESWLNRDASLYKLNFYDSTVGERTDEYNGRVQLDGNLEYRMVGLYAKGNVG
                     GRRDYAGNHGDATLYEGYGSLAPVDSFRVHVGKESLKWGKGYAWNPAALFTRPKNPDE
                     PELDLEGYYLARFEYTRSFEGPLKTVSFTPVFLPVFTGVNEDYAPRQDLESAAKLYLL
                     LFDTDLDFIYSYSLSSRSRFGFDFSRNITTNIEVHGEAAYLTRVSKTVLDDSGVVSVR
                     ESAAASFLAGIRYLNEADTTFIVEYYHNGAGRDTAVMKRQYVSINKAYAFYEMTGDDG
                     LLQKISDSADTAIAKVNPMRDYAYARISQKDPFDILYVTPAVTWIYNTADYSSTLSPE
                     IVYTGVTNLELRLKALFLIGEQYSESGEKPNKTRVELRVRYSF"
     gene            12294..13856
                     /locus_tag="PKJ16_08640"
     CDS             12294..13856
                     /locus_tag="PKJ16_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47095.1"
                     /translation="MNTILIEKIINRTRYLFVLFVALAGISSYTGGSAPVVYASIFAA
                     TGMYLAISIVHYLFIRRERIPLWLIMTMLTTDAILVFTVKFAFHFDPAHGYGMTIKEP
                     ATFLVYFIFGIVNALRYNKRMILYFGSLSVASYGLLVFLAIFDGGMFFSTDNALVFSS
                     NGMRVSHEFSKVLFLGVFTYFLYVMADFTNRNIRKIEDARGAADRNLSFASQLLGTVK
                     AVAHDLVSGSGELSDSTNSIGTTVDQTNKLIREITEISETFSKGIGEIRKKINTQEES
                     IEGNFAKIREISELMDEVYKSSAGQREMADGALSLAETNEQYVRESARLIMAMRDNSR
                     KIEDISKTINEIADQTNLLSLNAAIESARAGEYGRGFAVVADEISKLANISVESSKEI
                     STIIKSTVSNIEEVSKMVEGMSSGLTTIIDFVKRLTLFIQDLGTKTDREYRESRILYD
                     SNEEIKRTTGEVISHFNDQTELILRIMEWMEKMTSMTENISENLNALMILSHRLEGRS
                     VEITGMLDEVRS"
BASE COUNT         3129 a         3867 c         4005 g         2944 t
ORIGIN      
        1 ccaccagcgg ccccaccacc gtggcgaagg cgatgggcga ggcgatgccg aaaacggcga
       61 tcgcgaccgc gatggcgagc tcgaagttgt tgcccgcccc cgtgaaggcg agcgtcgtgc
      121 tcttgctgta atcggcccca aggcggcgcg ccatgaaaaa actcgccatg aacatgatca
      181 cgaaataaca ggagagcgga atcgcgatcc acagcacgtc gagcgggcgg ttgatgatct
      241 gttttccctg cgtgctgaac atgacgacta tcgtgaacag gagcgccacg agggtcatcg
      301 gtgaaatttt cgggatgaac actgtgtcgt accactcgtg tcccttgacg ggcacgagga
      361 tcacgcggct cagaacccca gcggcgaagg gaatgcccag atagatcatc acgctcacga
      421 agatgtcgcc gatcgtcacg ttcaccgccg tcccctgcag cccgaacagc ggcggcagga
      481 tggtgatgaa ggcccacgcg tatatgctga aggtgaggat ctggaagatg ctgttgagtg
      541 ccacgagccc cgcgccgtac tcgctgttgc cgccggcgag ctggttccac accagcacca
      601 tcgcgatgca gcgcgcgatc cccacgagga tgagccccgt catgaacagt tcccaccgcg
      661 ggtccggccc gaacaccgcc ggcgcgatga agccgaagaa cgctatcgac aatgcggtca
      721 taaggacggg gccaacgatc cagttctgca ccagggacag gcccagtatt ttcacgtccg
      781 cgaacaccgt gggcatgagc tcgtatttta ccttcgcaag gggcgggtac atcatgagga
      841 tgagtccaac ggcgatgagc atgttggtgt gctcgcccac ggtgagcgcc ttgttgaagc
      901 gctcgaccgg gccctggaag atgaatccaa tgacaatacc cacggccatg gcgaggaata
      961 tccagactgt gaggtagcga tcgagaaagg aaaggcgttt gctgatggct tcggacatac
     1021 tctgtctcct ggtcgtgttt tcttatgctg cggactagca catgagcgtg tcgatttcgg
     1081 attttatgta cgccgtgaat gcgggttcat cgttgtagta gtgccagacc cgttcgaggt
     1141 ttttccacga aatctccttt ccgtcgatca ccctcgatgc gacgaccgaa cgcgtcctga
     1201 gccggtagtc ggcaatatag cgctcattgc ccggcgcgtc cacgttcacg ggtctccaga
     1261 cgacggcgcc gcttgcgagc tctttcgaaa agtgtttctg caacgctatg ctggcaagct
     1321 cctcgatgcg cgtgcatgaa taacagcgaa actgtccgtg aaaataatac acgactgttt
     1381 ttacggttgg agctgccgaa agagagaagt gacttgctgg tgtaaaaacc gtgaaaccga
     1441 taaggacggc gatgatcata tttttcatct ggatactccg aaattgctgt caattggtta
     1501 aataattcat gaggcccgat aggaccggcg caccgagatg atcgttgggc accaagcggc
     1561 cgtttatgta tacccgcggc gtgccgtcga tgcccagccg gtcgccctct gcgacgtcgc
     1621 gggctacgat atcggctgtt tccttcgact gtatgcagga ttcgaacgtc gcctgcgcaa
     1681 gtccgcagcg gcgggcgatt tcacgcactg aggtttgggt gtgcttcacg ccgcgctcgg
     1741 agttgtcgta gagtgctcgg taaacgtccg ggtacttgcc cagccgccac gagcacagcg
     1801 acgcaacggc cgcgatgcag gcgccggccg agttgtcgtc gcggccgcgg tcgagtttcg
     1861 tgcagggacc gtcgagggga tagttcttat agattatggt gatcttgtcg ggccagcgct
     1921 cgcggagatc cgagagcgtg ttacccgcgt cgcggcagtg gtcgcaggtg aagtccatgt
     1981 agatgacgat gcgcaccgtc gcattggccg gccccaggcg cggcgagccg tcgaggtcga
     2041 tcgcgacagg cgccgtcgaa cgatagagcg agataagcgc gcgcacctgt tcgtctgttg
     2101 cgctctccga tagtcgcgca tcgaaggcgc gcttggacaa ttgcgccgag gcgatacccg
     2161 ccgtaaggag aacaagcgcc gttaccgcat acgggacaag acctgttcga accgatgcga
     2221 ttgcagtggc agcgatgccg tcaccaccgc gctctctagc ggaaaaccat ccggcaagcg
     2281 acagcataag cagtattgcg ttcgcgatat aggtatagga acacaacggg cacaccgcgc
     2341 gaatgaccgc aaccgagaga tacagaagaa ccaggttgcc ggcgaccgcc gtaagcgaca
     2401 ggaccgctcc aagtgcgaag ctcccgcgaa gccgtatcac atcatctgaa aaaagggacc
     2461 gaaggaacag cgcggccatg aaaccgtaga aggcgaaccc agcggccgcc acgggaatct
     2521 ccccgatgat cgggatgccg cgcaacgctg aatacgcgct ctccgcgacg gtccggcagg
     2581 agttcacgcc ggtgtgcgac gaggtgcaca ccgatttcac cgcgcccgag gccacgccgt
     2641 agaactcctg gatgagtgcg aatgagagtg ccatgcccgc aagggcgaaa acgacgagaa
     2701 gaacgagaag aactcgatgc gcgcgtttca tggcaagcct cacccaatca tgagctgcga
     2761 cacaacgagg tagatgccgc ctgcgatcac cagaacgccg cacgccttct tgagaatgag
     2821 cgcgcccttg gagctctcgt tccaattgag gtatttctgc accacctcag tgaaggtgcc
     2881 cgcgagaacg atcaccgcgc agtggccgat ggcatacgcc atgatgagcg aaacggcgaa
     2941 gacgagctgt gtcgcggcca cgctgaagac gacgcccagc atgggcgcca tgtaggcgaa
     3001 ggtgcatgga cccagcgcca ccccgaagat gagccctagg gcgagcgcgg ccaggagccc
     3061 cttcttttgg aagctggggt tcgccccctt ctcgagaaag ggcagcggga tgactcccag
     3121 caggtgaagt cccacgatga agaagatgac cgcgacgaaa taattccccc acgcgccgat
     3181 atcgcccatc atccgcccca tgaggccggt gatgaggccg atgatgccga tggtcacgag
     3241 gattcccagc gagaagagga gcgccagcgt gaaggcgcgc ttcgtcgtga tcgtgccctg
     3301 ctcgtcgatg aagcctacga tgagcgggat gctcgcgaga tggcacggtg agagcacgat
     3361 cgaaagcacc ccccagagga aggccccggc aagggcgatc ccggccgagt tggtgagcgt
     3421 gcccgagagc caggtgaaga gctcgagcac catcagttga cccccttcat cttgagcgcg
     3481 cggacgacct gctccttggg atagaacccc tcgtggcgaa agtactcgac gccattcttg
     3541 tcgaggaaga tctgcgtggg gatcacgcgg atgccgtact tcatcgcatc gcgcttgccc
     3601 tggggcgtcc acacgtcgtg gaaaacgacc ttcacctggc ccttgtattc ctcctcgacg
     3661 gctttcatga tgggctgcat catgcggcag gggatgcagt tcacggagcc gatctccacg
     3721 aaggtgacct tgtgttccac gggtccggct ttcttctccg gcatgccagc gggaacggct
     3781 gcaacgtcgt tggatgcaat agggacgggg gtgttctccg gctgttttgt atcgctgcag
     3841 ccgaacaggc tcccgaaaac gataaggggc gtttggcatg aacagccgtc cccggatttc
     3901 aggtggggaa gtgccgttac gatggcgatc gccgcgaggg cgggaatggt tactttaaga
     3961 attcttttca tggtctaaat cctttatggc gtaatggttt ttcaccggtt tcaccggcaa
     4021 gttttcatcg acttatgcgt caaggtggtt tttgaggccg ccctgcaact gcgtctcggc
     4081 gcggcgaagg aactcgcgta taagctcgat cgcctcatcc cgatccacat cgatgacgat
     4141 ggccttcatg cgcatgatat cctgggcttt aatattgatt aacgcgtccg ccatgccgtt
     4201 agcagttccc gccgcatgaa cagctgcccg cctgtttgtc cacccgctcc ttctgcggtg
     4261 accaggagcc gtcccgtatg gcaacgtaca tcttctcgac gacggcgtcg gacgcgggcg
     4321 ttgtcccctt ttcgatgccg agctcggtta ggataaccga ttgtggagta gcaccgacgc
     4381 gttcaagtac ccttttggca caagctgtag agcacccatc gatagcataa acgatatcgg
     4441 ctccttttgc cgactgaaca tagcccgcta gttcggcacc aactccggcc agacaggcca
     4501 ttttcgcaaa tccttcgtcc cgaagttttc gaacaacctt atctgcaatt tcgccaacat
     4561 tgctttgacc tgaacaggaa taaattagac ttgtaccgcc tgaacaacaa tccgccatgg
     4621 tgtaccctcc ttacaaaata aattttttga tttcttcaac ggacggcacc ttgccgacca
     4681 ccttcacctc gccatcaacc acgagcgcgg gcgtcatcat cacgccgtag ctcatgatat
     4741 ccttgatgtc cttgactttt aagatctccg catccacgcc gagatccgcg acggcctttc
     4801 gcgtgagctc ctcgagcttg atgcactttg cacagccggt tccaagtatt tcgagtttca
     4861 ttgagattcc tcctgggctt tatatgaaag cgtttccggc actgcagttt tctttgcaat
     4921 gccggaaaat catcgtcaag atctatgatg cgcggcagat atttcgtgct gcatgtgaat
     4981 tatcgatttc taccccgtcc ccttcctctg cttccctgtc cagaccaaag cggattgccg
     5041 gcagcatcac gcaggactag ctcggttgat ccttttttca cgaccttcgc gatgataagc
     5101 ttttcgccct gagcgttttt cacgctcgat ccggtgacct caacagcgtc gcctttcgca
     5161 aatgacattt ttcctccaat aaacgaagct ggcccgaggg caatacgaac ggttccagcg
     5221 cttgttgtaa tggtaagatg taagccgcct ccacgcctgc cgccggtgaa ggtctcgacg
     5281 agtgaaaccg cgcccttgac cgtttctacg gtagccgggt cgtagttgcg tcccgaggac
     5341 tggtaacgtc ggtaattccc actctgggcg aatacgctgt ctgtaacgaa agccgccgtc
     5401 atgagtgcca ggattgcgat aaataatgat ctcttcatat tgcgcctcct tgtgttctag
     5461 tgcccattag accctgtttc cagcgcttta gttcactttt tcacacgcac tgcaattgct
     5521 gttatcccga gccgcatgat ctcatttaca ggatcgttcg acttcaccat cacggtgtac
     5581 cggagcttct tgttcttcat gcggtcaggc acaccgccca catggagttt tactttaatc
     5641 tttccctcgc caccggcggg gatctccttc cgtgagagaa ggacgggggt gcagcctcac
     5701 ccggcgctca cccgttcgat cttgagcgtg cccgatccct tgttgcgaaa gacgaactcg
     5761 cagctctgct cggtcttctc gggcacctcg ccgaaatcgt actcaagctt ctcgaactcg
     5821 atggacggcc gcgaaaagaa aaacgccgtc gaaaagatga ccgcgagcgc ggcgacgcag
     5881 acggtcgcga gtggattcgc ggttgttatt gcacgcgcgc gtttcatcgt cccgtgcctc
     5941 cgttagaaaa acatgccaaa gatatatccg ctgatggtcg ccatgatgat caccagcgag
     6001 cagaatacga tcgtcttttg cgtgccaata tagttgcgga tcacgagcat gttcggtagc
     6061 gagagcgccg gtcccgcgag caggagcgcg agcgccggtc ccgcgcccat gccgctcccg
     6121 atgagcccct gcacgatggg cacctcggtg agcgtggcga agtacatgaa ggcccccacg
     6181 accgccgcga agaagttggc cgctagggaa tttccgccca ccgccgcgct cacccaggcc
     6241 gagggaatga tgccctcgtt gccgggacgt cccagcagga agcccgatac cagcaccccg
     6301 ccgaggagaa gcggcgtgat ctgtttggcg aagtcccagg tcgatgagaa ccactcccca
     6361 ccctcgccgt tgtcggtgct ggcgataatc gcgaggccga tggcgcccgc cgcgaaggga
     6421 atggtgggct cgtgcgaaaa aaagaacgcg agaacggcga ccggaaccga cgcggcgaca
     6481 actttccaca gtttgaaatc gaaccagagg acgagcgccg cggcgaagac gatcgccgac
     6541 gcggcggtga tgtaccattt ataggcgaac attgcgtacc agaagccgct cgtttcggca
     6601 ggctttcccc agttggcgaa aaccaggatg gccaccatcg aagcgaaata gacgatgtcc
     6661 ttccagagcg gccgctttgt ttcggtttgt ggcagttgca tggcggcatt gaccttttca
     6721 agctcctctt tccgatagat gaaatgcatt atcgccccga tcaccacgct gaaggcaacc
     6781 gcgcccaccg cccgggcgat gccgatttcg atgcccagca cgcgcgccgt gaggatgatg
     6841 gcgagcacat tgatggccgg tcccgaatac aggaaggcga tggccggtcc cagccccgcg
     6901 cccatacgat agatgccgga gaagagcggg agcacggtgc acgagcacac cgcgaggatg
     6961 gagcccgata ccgaccccac gccgtaggcg aggaccttgt tggccgttgg cccgaggtac
     7021 ttcatgaccg attcctgccg cacgaagacg ccgatggcgc ccgcgatgaa gaacgccggg
     7081 acgaggcaca ggagtacgtg ttcctgcgcg taccacttcg ccaggtataa tgcctcgaag
     7141 accgcattgt cgaaatgccg ggtgcccacc ggcatgtaaa acagcgcaag gaagaagccg
     7201 ccaatgaaca ggagcgcctt ccattcgatt ttccagtcaa ccttcattga gttactccat
     7261 tcggatatgt gttattgatg catatttgac aaaattacca ataatgagaa aaaaaatcac
     7321 cttcgtgcct tgcatgattt gagcacctcg ccctgctggc gcatgttgga ctcgatgaca
     7381 tcctcgatgc acccgaagaa ccccagtatg cagggggtct tgagatgata aaatacgctg
     7441 gttcccgatt tcttatcgcc gatgatgccg gcgtttttaa gaatggatag atgttttgat
     7501 acagtggaca tatcggcacc aatcatgtcg gtaaggtcat tgacgcagcg ttctcccttt
     7561 tccagcgctt cgattacaaa caagcgtgtg ggatgcgcca tcgccttgat gatggacgcg
     7621 cgcgccacat attgttttct tgttgcatcg ttcatcgaat caccattgtt ttcttaattg
     7681 acaatttagc caaataagaa atactcgtca atatatttta tcggaatcga agaaaaacat
     7741 tttactgctg ctgggggcgg cttgtggaaa tcaagggtac ggttatcgag cttgacgcgt
     7801 ttgacgttca gcgggcgatc ggaatcggaa tcgacgatga ccatgacaag gcgctcgagt
     7861 ttattaaaga agtcgacatc cccaggctca agcagcggct tgaacatcaa tgagttccgg
     7921 ttttcgaggc cagttaccgg cctcgaggga aataattgaa ccattattcg aaaatcaggg
     7981 tcaaatgcgt aatactattt ctcggatggg atcgcagcta tggtttggca gtatgttgct
     8041 gtgcaactcg gagtgatact gtcaagatgg gaagtgatga gattgataat ggataaggaa
     8101 gtgtaggata tggatttaat ttcggctcgt gacattacga agatttatcg aatcggcgag
     8161 gtcgagatca cggcgattaa aaacctgagc ttcaccatca gtccggcttc attcgtatcc
     8221 ttcgtggggc cctcggggag cggcaagacg acgctcctga acctcatcgg ctgtctcgat
     8281 atgcccacgg agggaaccat ggagatcgtc ggtgtgaaga ctccagcgct gggatggaag
     8341 gagcgggcga ccttcagagg cgagcatatc ggcttcattt ttcaggattt caacctgctg
     8401 ccggtgctga ccgtctatga gaacatcgaa tacccgctca tcatggtgca gaatgtcgaa
     8461 cccgccgaac ggaaaaaacg ggtgcttcgt ctgctcgacg aggtgggcat ggccgaccag
     8521 aaaggaaaat atcccgacca gatctctggg ggccagaagc agcgggtggc cattgcgcgc
     8581 gcattatcaa ccaatcccaa actcgtcctg gcggatgaac caaccgcaaa ccttgatcac
     8641 gccacggcat acatggtgct gggcttgatg aaaaagatgc gggatgaatt caataccacc
     8701 ttcatcttct cgacgcacga ccccaagatc gttggggagg ccgagatcat ctacacactt
     8761 gaggacggtg cattgaagtc gaaacaggtc gttaagaagg gtaaggccgc gagggggaga
     8821 aaacatgcat aatataatta aaattgcgat tcggaacctg gtcaggtacc gacggcggac
     8881 cctcctgacg gcttcgctca tcaccatcgg ggtggtattc gttatcgtgt tcattgccgt
     8941 atcaggctcc tttaaaaaca tgatgatcgg ccagattacg gattcgatgt tggggcatat
     9001 ccagatacat aaaaagggtt acgtgtcgtc gattgacaat ctccctctga atctcgcgct
     9061 caaagaagct gcggtgaagg agattggagc agcgctcgac ggcatgccga atgttgaagc
     9121 gtattcggtg cgtattaaat ttggcgggat gtttagcaat ttcgccgaaa cgactaacat
     9181 tcggctgaac gcggtgtatc ccgaaaagga attcaaaacg gttccgctcc tcatcgaccg
     9241 tatcatcgac gggaagaagg ggctccagcg tggtgagata ttcatcccgg aactcctggc
     9301 gaagggtatg aacgtcaaac cgggcgatac ggcggtgatt atcgcgacca atagggacgg
     9361 gtcggttaac ggaaaacaat tcatcgtcac gggtgtgctg ggaagcctgt ccggtccagg
     9421 cggaagggac ggttacattc attacgagga tggcgtggag ctgttgcgga tggatgaacc
     9481 ggaggcgagc gagatcgccg tgcggctcaa ggatttcgga gcgctctaca aagctgaagc
     9541 catgatgaaa aaacttttgg ttaaaaacgc caatggagac attgcggggc tgctcgaggt
     9601 gcatacctgg gagacgctct cgccgttttt caatatcgcc aaaatgatcg atgtgatgac
     9661 cgtttttatc cgcatcatgc tggtcgcgat tgtgctcgtg agcatcatga acgtcatgat
     9721 catggccgtc tatgagcgca tccgtgagat cggcaccatc gccgccatcg ggaccatgcc
     9781 gggcaagatt ctgtcaatgt tcatggtaga gggcctgtgc ctgggggccg cgggttcggt
     9841 actgggcgga atcatcgggg tgattgtcgt atttgcgatc aacatcgcca aggtgaaatt
     9901 taattttggc atgcaaaccg gtcttgttct taaagcgagt gtaaacccgt ccgatctgtt
     9961 tgctatatca ataatcgtca tcatcatttc gctgatcgcg agtctccagc ccgctttcaa
    10021 ggcgtcgcgc atggagccga taaccgcgtt gcgacacgta taaaaaagga gacagggcga
    10081 ggagggtgac atgataaaag caattcggct tgtaacgctc ataatgatga taagtccggc
    10141 agccacatat gccggcgaca atgagaggtt gttgaaggaa atcgatcgca atctcaatcc
    10201 gcaatcctac gagtcgtacc ggaagctcat caatattgag ccaggcggca ggaagaaaga
    10261 gtttgtcatg ttttcggtga agaagggtct tgaaaaaatg gtcgttctct ttatctcccc
    10321 cgccagcgaa aaggggcgga gcacgctgcg gctcggcgag aatatgtggc tttatatccc
    10381 caacgtggga aagcctgtgc ggatcaccag cctgcagtct gtggtcggcg gcgttttcaa
    10441 caatgccgat atcctgcagc tcgattatgc cgccgaatac aacgtcgaga cggtggaatc
    10501 gaaggggcag cagtacctgc tgcatttgaa agccaagacg aagacggttg cctatgaccg
    10561 tatcaggatg tgggcggata aagaacggaa gctccccgta aaaatcgaat gcctcacgga
    10621 aaaaaacatg ctcatcaaga ccctgcattt taaaaccatc aaggacttcg gcgggggtat
    10681 cgtccgtccc tcggtgatcg agacggacag cccgctctat aaaaactaca aatccatcat
    10741 ggttttcgcg aagctccgga agcgggtcgt gagcgacgag gtgttcacca tgacctttat
    10801 gccgcgcgtc gattcgctcc gaaaatgaag cgcctgttcc cggttctcct tctgctggtt
    10861 gcgacagtga ctgcagcata cggagaagac gagtatgcct tcgacgagag ggagctggga
    10921 gccgaaaccg aaaagaagcc ctatcatgtg ggaggtttcg ccgagctgcg gccggtcgaa
    10981 tcgtggctga accgagatgc ttcgctctat aagctgaatt tctacgatag caccgtcggc
    11041 gaacgtacgg atgaatataa cggcagggtg cagctcgacg gcaatctcga gtatcgcatg
    11101 gtgggcctgt acgcgaaggg gaatgtcggc gggaggcgcg actatgcggg caatcacggc
    11161 gatgccacgc tctacgaggg gtatggctcg ctggcgccgg tggattcctt tcgcgttcac
    11221 gttggcaagg aatccctgaa gtgggggaag ggctatgcct ggaacccggc ggcgctgttc
    11281 acccggccaa agaatccgga cgagcccgag ctcgacctcg aggggtacta tctcgcccgc
    11341 ttcgagtata ccaggagctt cgaggggccg ctcaagacgg tttcgttcac cccggtgttc
    11401 ctgccggtgt tcaccggcgt taacgaggat tacgccccgc gccaggatct cgaatcggcg
    11461 gcgaagctgt acctgctgct cttcgacacg gacctggact ttatttattc gtacagcctt
    11521 tccagtcggt cgcggttcgg gttcgatttc tcgcgtaaca ttaccaccaa tatcgaggtg
    11581 cacggcgagg ccgcatatct aacgcgggtg agtaaaaccg tgcttgatga ttcgggcgtc
    11641 gtttccgtgc gggagagcgc cgcggcatcg ttcctggcgg ggatccgtta tctcaatgag
    11701 gccgacacaa cgtttatcgt cgagtactat cacaacggcg ccggccgtga cacggccgtc
    11761 atgaagcgtc agtacgtgtc aatcaacaag gcatacgcat tttacgagat gaccggtgac
    11821 gacggtctcc tacagaagat ttccgacagt gccgataccg cgatcgcgaa ggtgaacccg
    11881 atgcgtgatt acgcgtacgc tcgcatcagc cagaaggacc ccttcgatat cctctacgtg
    11941 acgccggctg tcacctggat atacaatacc gccgattaca gctccacgct ttccccggag
    12001 atcgtgtaca ccggcgtgac caatctcgag ttgcgcttga aggcgctgtt tctcatcggc
    12061 gagcaataca gcgagtcagg cgaaaagcca aacaaaacga gggtggagtt gcgcgttcgt
    12121 tattcgtttt gaggttcgtg atcttcgtgt ctctcgaggc gtcaaccggt ccctcttttt
    12181 gctttttaag ggttttccct ccctgttccg ggaaaaggat cgaataaatg cttgatttcg
    12241 gtcaccctgc gggattatta ttaaaagaaa tctctgggcg aaaattgcga atcatgaata
    12301 cgatcctcat agaaaaaatc atcaaccgga cgaggtatct cttcgttcta ttcgtcgcgc
    12361 tggcgggcat ctcgtcgtat accggcggct cggcgcccgt agtgtatgcc tccatttttg
    12421 ccgcgaccgg gatgtatctg gcgatctcaa tcgtgcacta tctgttcatc cggcgggagc
    12481 gcatccccct gtggctcatc atgacgatgc tcaccaccga cgcgattctc gttttcacgg
    12541 tcaaattcgc ctttcatttc gatcccgcgc acggctacgg catgacgatc aaggagccgg
    12601 ccacgttcct ggtgtatttc atcttcggaa tcgtcaacgc gctcaggtat aacaagcgga
    12661 tgatactgta tttcggttcc ctgtcggtcg cgagctacgg tcttctcgtt tttctcgcaa
    12721 tattcgacgg cggcatgttt ttcagcaccg ataacgcgct cgtcttctcg agcaacggca
    12781 tgcgggtttc ccacgaattt tcgaaggtgc ttttcctcgg ggtgttcacg tacttcctgt
    12841 acgtgatggc ggacttcacc aaccggaaca tccggaagat cgaggacgcg cgcggcgcgg
    12901 cggacaggaa cctctccttc gccagccagc tcctgggcac cgtgaaggcc gtggcgcatg
    12961 atctcgtatc cgggagcggc gagctcagcg actccaccaa cagcatcggc accaccgtgg
    13021 accagaccaa caagctcatc cgtgagatca ccgagatctc cgagaccttc tccaagggga
    13081 tcggggagat ccgcaagaag atcaatacgc aggaggagtc gatcgagggt aacttcgcca
    13141 agatccgcga gatatccgag ctgatggacg aggtgtacaa gtcgagcgcg ggacagcgcg
    13201 agatggccga cggcgccctc tcgctcgccg agacgaacga gcagtacgta cgcgagagcg
    13261 cgcgtctcat catggcgatg cgcgacaact cgcgcaagat cgaggacatc tccaagacca
    13321 tcaacgagat agccgaccag accaacctcc tgtcgctcaa cgcggccatc gagtccgcgc
    13381 gcgccgggga gtacgggcgc gggttcgccg tcgtggccga cgagatctcg aagctcgcga
    13441 acatcagcgt cgagtcgtcc aaggagatct ccaccatcat caagagcacc gtgagcaaca
    13501 tcgaggaggt ctcgaagatg gtggagggga tgtcgtcggg cctgaccacc atcatcgatt
    13561 tcgtgaagcg cctgacgctg ttcatccagg acctgggaac gaagaccgat cgggagtacc
    13621 gcgagagccg catcctgtac gactccaacg aggagatcaa gcgcaccacg ggcgaggtga
    13681 tctcccactt taacgaccag accgagctga tcctgcgcat catggagtgg atggagaaga
    13741 tgaccagcat gaccgagaac atctccgaga acctgaacgc gctcatgatc ctgtcgcacc
    13801 ggctcgaggg gcgctcggtg gaaattacgg gcatgctcga cgaggtgcgt tcgtgacggc
    13861 gggaccttcg tctccggccc atgtccgcgt cggcgagtac gactgctact cgctccccga
    13921 tggtttcttt cgcctcgacg ggggc
//
LOCUS       DAORHF010000083        13916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3457_length_13916_cov_5.682130, whole genome shotgun sequence.
ACCESSION   DAORHF010000083 DAORHF010000000
VERSION     DAORHF010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13916
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3457_length_13916_cov_5.682130"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="PKJ16_08645"
     CDS             <1..1085
                     /locus_tag="PKJ16_08645"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV47096.1"
                     /translation="IFKRFEDAFRRLNITLVDAFTHGKGNGDPVFLDFYHQDDIDTTR
                     VICVESPRDIPRFMEIMNEVQSGNQSGAFYIFDSLTGMNELWKDERAVLDFFTFTCPK
                     LYDLNTIAYWVYEKDAHAREFIAGLTHITQVVLTVNNTSAGYYELRILKLEDRPSSHA
                     GGAHFFSIVNRQVCFQDGRSENLYNIGRKVRELRKSAGITQAELAARMGLTPGAVSQI
                     ENDLITPSLQTLVQLTVIFSRPFEYFIDAAAAERGTGPVVSKKRLVKGAEGGALTVYA
                     LWDSGDIGIRPYVVSIAHEGVVDGPIMLYKGREFVTVISGVLNITVGGADRVLRKGDS
                     IALGDDFAERWRNAGKGECEFYYVQF"
     gene            1151..2212
                     /locus_tag="PKJ16_08650"
     CDS             1151..2212
                     /locus_tag="PKJ16_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HNV47097.1"
                     /translation="MDHQHAHDKQLPPEQMIQTLEKKVYDLRNLIEMGMSLTSNLVFE
                     RLVESILYSCIGQMFVEKVAILLQVDIDIDNYHVHTAKGYDQDVVDGAVMLVENAPLV
                     RYLNENAAPQDFTLLWENETLRSDLSRMEVLSPELIVPMKSKNQLNGILVLGPKITAE
                     NFSSDERDFLTDLAKFAAIGVENSRLYLMATLDRMTRLYIHHYFQERLTEEVKRSHRY
                     NTPMTLLISDIDHFKGFNDTYGHQQGDIVLKEVAKLFKQNLRVMDVPARYGGEEFAVI
                     LPETALADGVKVANRLRKCVEEHEFPGQGEPLHVTVSIGVAQYDPALNEERAELIKRA
                     DAAMYSAKHAGRNKVAVHK"
     gene            2209..3078
                     /locus_tag="PKJ16_08655"
     CDS             2209..3078
                     /locus_tag="PKJ16_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47098.1"
                     /translation="MSRRPNTGGDAARRDLRRRFDTARDLERTGDFPAALREYAAIIK
                     QHRDCKDAFQNLGALYTRMSCPNKALSCFKRAVALGEDYLSYFNLGCLYYTKGDFKRA
                     VINLDRSRRLNPDFMLSLELMALSFTRLKNYRAAETSFDEVLRRAPRNQVALTAMAIL
                     AFDRRSYDRSLEYLNRIIVIDLGNAKMRAFRARVLARLNRFTESASELKEAKRAGRGF
                     RAFDAFVRSVPVERYTDRFGSLEEKLGAVRAAASDRRDSGSLIALSLCHILSGETDAA
                     LDFLVEARRRAAH"
     gene            complement(3094..4629)
                     /locus_tag="PKJ16_08660"
     CDS             complement(3094..4629)
                     /locus_tag="PKJ16_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HNV47099.1"
                     /translation="MNDHAAIANRNTALLAEIGRCFTASAELDELLHEVLAATARHLH
                     IVRGMISIHDPETDDIVADVSYGYTEEETARGRYRPGEGIIGTVIATGKPAIVPSIAA
                     EPRFLNKTGARGRDGDEDLSFICIPIVSGDTAIGAIAADMPLRAPEPLEDTAQLVTTI
                     AIMIAHAVNSRRAMLQRERLLREENRLLRMKLSIDDAPQPIIGTSRAMRELTDKILLV
                     AETDTTALITGESGTGKELVADAIHYASRRKNGPFIKVNVAALPHNLIESELFGYEKG
                     AFTGAARQKKGRFELAEGGTIFLDEIGDLDAHVQIHLLRVIQERTIERLGGTRTIPID
                     VRIIAATHQDLEQKTGDGSFRSDLYYRLNVFPVHCPPLRERAADIVLLADHFLERYTR
                     KFDKAITRISSEAIDLLTAYHWPGNIRELENCIERAVIMSREDVIRSYHLPPSLQMAQ
                     PAAGRAVTLEEMTNRFLREIITDHLKMTRGNVTRAAALLGTTKRILAYRIAKLGIDCA
                     QLK"
     gene            complement(4851..5834)
                     /locus_tag="PKJ16_08665"
     CDS             complement(4851..5834)
                     /locus_tag="PKJ16_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47100.1"
                     /translation="MKRTIIGILGVTLLVVALSGSMLGAEEAKGESKPFGISAGVDYW
                     TLYFWRGDLLYGENEDVFFPYVKWAVFDTGVTVGWTGEYARNSRSVPLKEKNAADFFV
                     AYDGKFIGKEGEEKLLVGASLWYYYWYNSGYYTEEYPLPDVYPGRKEDGSINNRIDPN
                     KDNQYEARYDASYWTGTVYVGLNVPLHPTVIYNHDYYSNNQEGTRRVASDWYLTLKVS
                     EAFQLAKNVSLTLSCAASYYKNVYADDLRTWKNETREGWSDITSSAMLTVTLGNASLF
                     GSGSWANRPNKGWYRVADGYPFAGTPGGHGDGRVFTITNVYWAAVGASYTL"
     gene            6178..6945
                     /locus_tag="PKJ16_08670"
     CDS             6178..6945
                     /locus_tag="PKJ16_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV47101.1"
                     /translation="MAARGAHIAAAALIAVYVAAPASCSRGVSKHYRAGETLYVLAER
                     GLALREAPGTDAKVRAAVSRGASVVVESVPFFQRARREGTMRGYWVAVRVDGRRGYLF
                     DAFLCRVPPYRAGEGRDAFLTRAFGAHTTEERENDETTTVERRYAGGVRVVITRSGID
                     CVEKVEYLVPAIRIETAFLLFRPLLSGLGSIDAFPDRTQVLDLSNDRDGSEQLNIVVR
                     TDGEEVRGIEFMPDGIAYTCAIARHGDGVRVEECFND"
     gene            complement(6956..7951)
                     /gene="tatC"
                     /locus_tag="PKJ16_08675"
     CDS             complement(6956..7951)
                     /gene="tatC"
                     /locus_tag="PKJ16_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV47102.1"
                     /translation="MAVKKGGKPAKKGPPGGGKGGARVPAKRSASSKRGPGSSREEEM
                     SAPVLADALPAGYPDGNERPHDDRTATSAAPGDGGEKDMGSYVRGAMGHLDEFRSRII
                     LPVIVVLVLTVAGFFLSDHLLAFISRPYLALGFTLNVFNLIESFMLRVKVALIAALLA
                     GVLVIIVQLWRFVLPRVDRRDRMFVRLSFLAALLLFYGGVAVTYVFLLPMAIKFLITL
                     TPEHMANTISAGKYLSFVTMFCFGLGAVCELPIVVLVLTKMGMVTPAFLSSKRKIAIV
                     LIWVVAAIITPTVDPFTQAAVAVPLMLLYEISIMLSRLVIVRKKKREQEDEAAYA"
     gene            complement(8028..9398)
                     /locus_tag="PKJ16_08680"
     CDS             complement(8028..9398)
                     /locus_tag="PKJ16_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HNV47103.1"
                     /translation="MDTIHGIIKLINGYLWGPPMLVVLFGTHLFLTVRLRGIQRYVFL
                     AIRLSFQKEDDGHGDVSHFGSLTTALAATIGTGNIVGVATAVAMGGPGAVLWCWLTGV
                     FGIATKYAEALLAVKYRVRTEDGTMLGGPMYALEKGLGKKWLAVVFCVFTALAAFGIG
                     NMVQANSIAVMANETFSIDPWITGLALAALTAIVILGGIRSIARVCEVLVPFMAAFYV
                     LGCVVILVINREFLMQTLSLIVTAAFTPQAAGGGFVGSTIMTAARFGIARGLFSNESG
                     LGSAPIVAAAAKTRNPVRQALVSSTGTFWDTVVVCALTGLVLVSTIIAHPEMTVGTNG
                     AALTKAAFSKIPTVGPIVLTVGLLTFVFSTILGWSYYGERAVEYLFGKIAIRPYRLAW
                     VVAVMAGSVVSLPLVWDLADAMNAMMALPNLAALLLLNGVIVLETKKYLWDDNLHLAM
                     EHDEVV"
     gene            9762..11123
                     /locus_tag="PKJ16_08685"
     CDS             9762..11123
                     /locus_tag="PKJ16_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HNV47104.1"
                     /translation="MKKRFALIAFLLIGMIALAPLGAQEAAKAAAPKELHSSGDFIWI
                     MIAAFLVFFMQAGFAMVETGFTRAKNAGNIMMKNLFDFSMGSIAFFVVGYGLMFGATK
                     LGLIGSSGFFLADASPKPDADGGNAGLWQYAYFLFQLVFAATAATIVSGAMAERTKFV
                     SYLAYSLVISALIYPVSGHWIWNANGWLAKLGFQDFAGSTVVHMVGGVAAMMGALVLG
                     PRYGKYGPDGKPRAIPGHNIPLAALGVFILWFGWFGFNPGSTTAATPSIAFIAINTNL
                     AAAAGALASMAAIWIQTGKPDVSMSLNGALAGLVAITAGCNFVSPASSIVIGLIAGVV
                     VVLAVQLFDFVFKIDDPVGAISVHGVCGALGTILVGVFGDATYGGMNGLINGGGFKLL
                     GIQALGALVVFGWVAVTAGILFLVIKKTIGLRVSREEELKGLDIEEHGMESYSGFQIF
                     RNQ"
     gene            11136..11477
                     /locus_tag="PKJ16_08690"
     CDS             11136..11477
                     /locus_tag="PKJ16_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HNV47105.1"
                     /translation="MKLITAYIQPEKLNDVKQELFKEQISKMSVTNSLGCGQQGGYTE
                     TYRGVPMEVNLLHKVRIEIAVNDDYVDRTVKAIIRGAKTGKIGDGKIFVVKLENCIRI
                     RTEEEGKEAIG"
     gene            complement(11597..>13916)
                     /locus_tag="PKJ16_08695"
     CDS             complement(11597..>13916)
                     /locus_tag="PKJ16_08695"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47106.1"
                     /translation="LMGMSAFDLVHPDDVPAALAMFRDGFSDGRGGRIELRFRRADGT
                     YAWLECDGTVMTNESGARSVLVASREIDDRKAAEKALSASEERYRTIVETIEDGYYEV
                     DLKGDFTFCNGSMYRMLGYEPGALIGLNYRAYMDGENSRRIFTVFNRVFREGRPVRSF
                     DWELIRRDGERISAETSVSLITDHDGAPRGFRGVVRDITERKRFEARLRESEERYKQL
                     VTHAGDVIISFDALGFFTYLNPTALARMQCEEREILGRHFSEFVHPDYRDEVIEFYRA
                     QYLGRIPSTYNEHPVVARDGTVYWIGQNVQAIFKDDAIVGFQGIARDITERRAAEEKL
                     WQNEIRLRLQHMALATLARTESLYAGDLKGSFRAITTSGAHNLDIERSSIWLFDRNRT
                     RIICYDSFAATHDSHRSGWEIAVKDFPEFFDAIERDRLIDATDAAADERTRAIVARHL
                     ARLGIGALLAVPFRLGGMTSGILSLEHVGPPRLWTPDEENFAGSLSDFASLALESYNR
                     RLAEEALRANEEELRERNEIIESDIKNAQLIHQELLPSAPPRYPGIACDFRNASLEAV
                     GGDYFSFTTLQEGGLGVFIGDISGHGVSAALFITLVKATADRICRAHGQNPLEFIRTL
                     NRDLADTMANNFLTAIYGYFQEIGRSRFRFTFSRGGHPHPLLYRAATGSVEMIKSRGT
                     IIGKFDDFEFTDERVDLATGDRVFLFTDGLPETRNERYEIIGYDDLPALFERLTAPGL
                     GETLDRIIDHVREFRGFAPIEDDLVIIGFEIV"
BASE COUNT         2460 a         4306 c         4592 g         2558 t
ORIGIN      
        1 ccatcttcaa gcgctttgag gacgcttttc gccgcctcaa cataaccctc gtggacgctt
       61 ttacgcacgg caaggggaat ggtgatccgg tgttcctcga tttttatcat caggacgata
      121 tcgacacgac gcgggtgatc tgcgtcgagt ccccgcgcga tattccccgg tttatggaga
      181 tcatgaacga ggtgcagagc gggaatcaga gcggggcgtt ctatatattc gacagcctca
      241 ccggcatgaa cgagctgtgg aaggacgagc gcgcggtcct cgactttttc accttcacct
      301 gtcccaagct ctacgatctc aacaccatcg cctattgggt gtacgaaaaa gacgcgcacg
      361 cgcgcgagtt catcgcgggc ctcacgcaca tcacgcaggt ggtgctcacg gtgaacaaca
      421 ccagcgccgg atactacgag ctgcgcatcc tcaagctcga ggatcgtccc tcgtcgcatg
      481 ccgggggcgc gcattttttc tccatcgtaa accgccaggt gtgcttccag gacgggcgtt
      541 ccgagaacct gtacaatatc ggacgcaagg ttcgcgagct gcgcaagtcg gcaggcatca
      601 cgcaggcgga gctggccgcg cggatgggcc tcacgccggg ggcggtctct cagatcgaga
      661 acgacctcat caccccctcg ctccagacgc tcgtgcagct caccgtgatc ttcagccgtc
      721 cattcgaata cttcatcgac gcagcggccg cagagcgggg gacggggccg gtcgtgtcaa
      781 aaaagcgcct ggtaaagggg gccgagggcg gggcgctcac ggtatatgcc ctctgggaca
      841 gcggcgatat cggcatacgc ccctatgtgg tgtcgattgc ccacgagggt gtggtggacg
      901 gccccatcat gctctataaa ggaagggagt ttgttacggt gataagcggc gtcttgaaca
      961 tcaccgttgg tggggcggac cgggtgttgc gcaaggggga ctcgatcgcg ctgggcgacg
     1021 atttcgccga gcgatggcgc aatgccggaa agggagaatg cgagttttat tacgtgcaat
     1081 tttagctcaa tacgcgcgtt gtgttgttcg ataattaatg gagcgctccc acgggaggca
     1141 cgatccgacc atggaccatc aacacgcaca tgacaaacag ctcccgccgg agcagatgat
     1201 ccagaccctc gagaagaagg tctacgatct ccgcaatctc atcgagatgg gcatgagcct
     1261 cacctcgaac ctggtgttcg agcgcctggt ggagtccatt ctctacagct gcatcgggca
     1321 gatgttcgtg gagaaggtgg ccattctgct gcaggtcgat atcgacatcg acaactacca
     1381 cgtgcacacg gccaaggggt acgaccagga cgtggtggac ggcgccgtca tgctcgtcga
     1441 gaacgcgccg ctcgtccgct acctgaacga gaacgccgct ccgcaggatt tcacgctcct
     1501 ctgggagaac gaaacgctcc ggtccgatct ctcgcgcatg gaggtccttt cgcccgagct
     1561 catcgtgccc atgaaatcca agaaccagct caatggcatc ctggtcctgg gccccaagat
     1621 caccgccgag aacttctcct cggacgagcg cgatttcctc accgacctgg cgaagttcgc
     1681 ggcgatcggc gtggagaact cgcggctgta cctcatggct acgctggacc gcatgacgcg
     1741 cctgtatatc catcactatt tccaggagcg gcttacggag gaggtgaagc ggagccatcg
     1801 ctacaatacg cccatgacgc tgctcatttc cgacatcgac cacttcaagg gctttaacga
     1861 cacctacggc caccagcagg gcgacatcgt cctcaaggag gtggcgaagt tgttcaagca
     1921 gaacctgcgc gtgatggacg tccccgcgcg ctacggcggg gaggagttcg cggtgatcct
     1981 tccggagacc gcactggccg acggggtgaa ggtcgccaat cgacttcgca aatgcgtcga
     2041 ggagcatgag ttccccggcc agggcgagcc gcttcatgta acggtgagca tcggcgtggc
     2101 ccagtatgat cccgcgctga acgaggagag ggcggagctc atcaagcggg ccgacgcggc
     2161 gatgtacagc gccaaacacg ccggccgcaa caaggtggcg gtgcacaagt gagccgacga
     2221 ccgaacaccg gcggcgacgc tgcgcgccgc gatctccggc gccggttcga tacggcgcgc
     2281 gacctcgagc gcacgggcga tttcccggcc gcgctgcgcg agtacgccgc catcatcaaa
     2341 caacaccggg attgcaagga cgcgttccaa aacctgggcg cgctgtatac gcgcatgagc
     2401 tgtcccaaca aggcgctctc gtgcttcaag cgcgccgttg cgctgggcga ggactacctg
     2461 agctatttta acctgggatg cctctactat acgaagggcg atttcaagcg cgcggtgatc
     2521 aacctcgacc gctcgcggcg cctcaatccg gacttcatgc tgtcgctgga gctcatggcg
     2581 ctcagtttca cgcggctcaa gaactaccgt gcggccgaaa cgagcttcga cgaggtgctg
     2641 cgccgcgcgc cgcgcaacca ggtcgcgctc accgccatgg ccattctcgc cttcgatcgg
     2701 cgcagctacg accgatcgct cgagtatctc aaccgcatca tcgtgatcga cttgggcaat
     2761 gcgaagatgc gcgccttccg ggcgcgggtc ctggcgcgtc tcaaccgctt tacggaatcg
     2821 gccagcgagc tcaaggaggc gaagcgcgcc ggacgggggt ttcgcgcctt cgacgccttc
     2881 gtgcggtccg ttcccgttga gcgatacacc gatcgcttcg ggagcctcga ggagaagctc
     2941 ggcgccgtcc gcgcggccgc gagcgaccgg cgcgacagcg ggagcctcat cgccctcagc
     3001 ctctgccaca tcctctccgg cgagaccgac gccgcgctcg actttctcgt cgaggcgcgc
     3061 cgacgcgccg cccactagcg gccggtcccg ccctcacttc aactgcgcgc agtcgatccc
     3121 cagcttcgcg atccgatagg ccagaatccg cttggtggtc cccagaagcg ccgcggcgcg
     3181 cgtgacattt ccccgcgtca tcttcaggtg gtcggtgatg atctcgcgga ggaagcggtt
     3241 ggtcatctcc tcgagcgtga ccgcccgtcc ggctgcgggc tgcgccatct gcaatgacgg
     3301 cgggagatgg tagctgcgga tcacgtcctc gcggctcatg atcaccgcgc gctcgatgca
     3361 gttttccagc tcgcgaatgt ttcccggcca gtggtacgcc gtcaggaggt cgatcgcctc
     3421 gctcgagatg cgggtgattg ccttgtcgaa cttgcgcgtg tagcgctcca ggaagtggtc
     3481 ggccaggagg acgatgtcgg cggcgcgctc gcgaaggggc gggcaatgca ccgggaacac
     3541 gttgagccgg taatagagat cggagcggaa ggagccgtcg ccggtctttt gctcgaggtc
     3601 ctggtgggtg gcggcgatga tccgcacgtc gatgggaatc gtgcgcgtgc ccccgagccg
     3661 ctcgatggtg cgctcctgga tcacccgcag gaggtggatc tgcacgtgcg cgtcgaggtc
     3721 gccgatctcg tcgagaaaga tggtgccgcc ctcggcgagc tcgaagcggc ccttcttctg
     3781 ccgcgccgcg ccggtgaagg cgcccttctc gtacccgaac agctcgctct cgatgaggtt
     3841 gtgcggcagc gcggcgacgt tcaccttgat gaaggggccg ttcttccgcc ggctcgcgta
     3901 gtggatcgcg tcggccacga gctccttgcc ggtgccgctc tccccggtga tgagtgcggt
     3961 ggtgtcggtc tcggccacca ggaggatctt gtcggtgagc tcgcgcatgg cccgcgacgt
     4021 tccgatgatg ggctgcgggg cgtcgtcgat cgacagcttc atacggagca gccggttctc
     4081 ctcgcggagc aggcgctcgc gctgcagcat ggcgcggcgg ctgttcaccg cgtgcgcgat
     4141 catgatggcg atggtggtca ccaactgcgc cgtgtcctcc agcggctcgg gcgcgcggag
     4201 gggcatgtcg gcggcgatgg cgccgatcgc cgtgtccccg ctcacgatgg ggatgcagat
     4261 gaaggacagg tcctcgtcgc cgtcgcgtcc gcgcgcgccg gtcttgttca ggaagcgcgg
     4321 ttcggcggcg atggagggaa cgatcgccgg tttgccggtc gcgatgaccg tcccgatgat
     4381 cccttccccc ggccggtagc gcccgcgggc ggtctcctcc tcggtgtagc cgtatgatac
     4441 gtccgccaca atgtcgtcgg tctcggggtc gtggatgctt atcatccccc ggacgatgtg
     4501 cagatgccgc gccgtcgcgg caagaacctc gtgcaggagc tcgtccagtt cggcgcttgc
     4561 ggtgaagcac cggccgatct cggcgagtag ggcggtgttt cggttcgcga tggctgcgtg
     4621 gtcgttcatg gcaatgcggt tctccatacc ggctcatcga cccctcggaa aaggggtgcg
     4681 atcgctcgat ggagacacta tggcgctctc gcggtccttt aaaaataaaa aaatctccag
     4741 agcggctctc cggggaacta tttaacaaaa atgtaacgtt acgggaggcg gtggcggatg
     4801 atgcgcggac agcgcgtgtg actgcgctgt ccgcgtggtc tggaaatcca ttacagggtg
     4861 tatgatgccc caacggcggc ccagtagacg ttggttatgg tgaacacccg cccgtcgccg
     4921 tgcccgcccg gggttccggc aaaggggtat ccgtccgcaa cccggtacca gcccttgttg
     4981 ggccgattcg cccagctgcc gctcccaaac agggacgcat tgcccagcgt tacggtgagc
     5041 atggcagagc tggtgatgtc gctccagccc tcgcgcgttt cgtttttcca ggtgcgcagg
     5101 tcgtccgcgt acacgttctt gtagtacgac gcggcgcagg aaagggtgag gctcacgttt
     5161 ttcgcaagct ggaaggcctc gctcaccttg agggtgaggt accagtcgct cgcgacgcga
     5221 cgggttcctt cctgattgtt cgaataataa tcgtgattgt agatcaccgt cggatgaagc
     5281 ggcacgttga gccctacgta caccgtgccc gtccagtaac tggcgtcata gcgcgcttcg
     5341 tactggttgt ccttgttcgg gtcgatgcgg ttgttgatgg aaccgtcctc ctttctcccg
     5401 ggatacacgt ccggcaacgg atactcctcg gtgtagtagc cggaattata ccagtagtag
     5461 taccagaggc ttgcgcccac caggagtttc tcttcccctt ccttgccgat gaacttcccg
     5521 tcgtaggcga cgaagaagtc ggcggcgttc ttttccttca gcgggacgct gcggctgttg
     5581 cgtgcgtatt ccccggtcca gcccacggtc acgcccgtgt cgaagacggc ccacttcacg
     5641 tatgggaaaa acacgtcttc attctcgccg tagagcagat cgccgcgcca gaagtagagg
     5701 gtccaatagt cgacccctgc cgatatgcca aacggcttgc tctcgccctt cgcttcctct
     5761 gcgcccagca ttgacccgga aagcgcgacg acaagcagcg tgacgcccag tattccgatg
     5821 attgttctct tcatagcaaa ctccttttac agttatttgc cactctatac agcacggaac
     5881 gtgccaaaca catcgcatgt ggcgcgagtt aacattactg taataattga gtgaaatttc
     5941 atgcgagtct cgtgctggcg gggcggggcg ggggtgatga tggcgcggtt cgatgcacga
     6001 cgcctgtgcc tgggcgcggc gcgcatcgat tctgatccgt caggaacgac agaaatgttc
     6061 catgtcgcgg tacgatggca caatcgtgtg ccgcgctaca tccatcaaac gaattcggat
     6121 tgacaaatgc aagtgcgcga ctgcgtgatt ggcccgatgg gaagtaaaca gcaaagaatg
     6181 gcggcgcgcg gagcgcacat cgcggcagcg gcgcttatcg cagtgtatgt cgcggcgccg
     6241 gcgtcgtgtt cgcgcggcgt ttcgaagcac taccgcgcgg gggagacgct ctacgtgctc
     6301 gcggagcggg ggcttgcgct tcgcgaagcg cccggtacgg acgcgaaggt gcgtgcggcc
     6361 gtatcgcgcg gggcgagcgt cgtcgtggag tcggtgccgt tcttccagcg ggcgcgtcgc
     6421 gaggggacga tgcgcggcta ctgggtcgcc gtgcgcgtcg atgggaggcg cgggtacctg
     6481 ttcgacgcct tcctctgccg cgtgcccccg tatcgcgccg gggaggggcg cgacgccttc
     6541 ctcacgcgcg ccttcggcgc ccacacaacc gaggagcgcg agaacgacga gacgacgacc
     6601 gtcgagcggc ggtacgcagg cggcgtacgg gtcgtgatca cccgatccgg gatcgactgc
     6661 gtcgaaaagg tcgagtatct ggtgcccgcc atcaggatcg agacggcctt ccttctcttc
     6721 cggccgctcc tgtccggcct ggggtcgatc gacgcgtttc ccgaccgcac gcaggtcctc
     6781 gacctgtcca atgaccggga cggatcggag caattgaaca ttgtcgttcg aaccgacggc
     6841 gaggaggtgc gcgggatcga attcatgccg gacggcatcg cctatacctg cgccatcgcg
     6901 cgccatgggg acggcgtgcg cgtcgaggag tgcttcaacg actgaccggc gccgcctatg
     6961 cgtacgcggc ctcgtcctcc tgttcccgct tcttcttgcg cacgatcacc agccgcgaca
     7021 gcatgatgga aatctcgtag agcagcatga gcgggacggc gacggccgcc tgcgtgaagg
     7081 ggtccaccgt cggggtgatg atggccgcca cgacccagat gaggacgatc gcgatcttcc
     7141 gcttgctgct caggaatgcc ggggtgacca tgcccatctt cgtgagcacc agcaccacga
     7201 tgggcagctc gcagaccgcg cccaggccga agcagaacat ggtcacgaag ctgaggtatt
     7261 tcccggcgct gatggtgttg gccatgtgct cgggcgtgag cgttatgaga aacttgatgg
     7321 ccatggggag caggaagaca taggtgacgg ccaccccgcc gtagaagagg aggagggccg
     7381 ccaggaacga caggcgcacg aacatgcggt cgcgccggtc cacgcgcggc agcacgaagc
     7441 gccagagctg gacgatgatg acgagcaccc ccgccagcag cgcggcgatg agcgcgacct
     7501 tcacgcgcag catgaacgac tcgatgaggt tgaagacgtt gagcgtgaac ccgagcgcga
     7561 ggtacgggcg cgagatgaac gcgagcaggt ggtcggagag gaaaaacccg gcgacggtga
     7621 gcaccagcac cacgatgacc ggaagaatga tgcgcgagcg gaactcgtcg aggtggccca
     7681 tcgccccgcg gacataggag cccatgtcct tctcgccgcc gtcgcccggc gcggcgctcg
     7741 tcgccgttcg atcgtcgtgc ggccgttcgt ttccgtccgg gtatcccgcg gggagcgcgt
     7801 cggcgagcac cggcgcgctc atttcttctt cgcgcgatga gcccggcccg cgtttcgagg
     7861 aggcgctcct tttcgcgggg acgcgcgcgc cgcccttccc gccgccgggt gggcctttct
     7921 tcgccggttt tccgcctttt ttcacagcca cagggtgctc cgtaatcgct cgtccggacc
     7981 ttcataggcc ggcacggatt tttgtaaaca tcaaaacggc cggggggcta cacgacctcg
     8041 tcgtgctcca tggcgagatg cagattgtcg tcccagagat atttcttggt ctcgagcacg
     8101 atgaccccgt tgagcaggag caatgcggcc aggttgggga gcgccatcat cgcgttcatg
     8161 gcgtcggcga ggtcccagac cagcggcagc gacacaacgg agcccgccat cactgcgaca
     8221 acccaggcga ggcggtacgg ccggatggcg atcttcccga agaggtactc gaccgcgcgc
     8281 tcgccgtaat acgaccagcc caggatggtg gaaaagacga aggtgaggag cccgaccgtg
     8341 agcacgatgg ggcccaccgt gggtatcttc gagaaggccg ccttggtgag cgccgcgccg
     8401 ttggtcccca cggtcatttc ggggtgtgcg atgatggtgc tcaccagcac cagccccgtg
     8461 agggcgcata cgaccaccgt gtcccagaag gtcccggtgg aggacaccag cgcctggcgt
     8521 acgggattgc gcgttttcgc cgccgcggcc acgatgggcg ccgagcccag gccggactcg
     8581 ttcgagaaca gtccccgcgc gatcccgaag cgcgccgccg tcatgatggt ggagcccacg
     8641 aagccgccgc cggccgcctg gggcgtgaat gccgccgtga cgatgagtga aagcgtctgc
     8701 atcaggaatt cgcggttgat gaccaggatg acgacgcatc ccagcacgta gaaggccgcc
     8761 atgaagggga cgagcacctc gcagacgcgc gcgatcgatc ggatgccgcc caggatcacg
     8821 atcgcggtga gggcggccag ggccagtccc gtgatccacg gatcgatgga gaaggtttcg
     8881 ttggccatca cggcgatgga gttggcctgc accatgttgc cgatgccgaa ggcggcaagg
     8941 gccgtgaata cgcagaagac caccgccagc cactttttcc ccaggccctt ctcgagggcg
     9001 tacatggggc cacccagcat ggtgccgtcc tcggtcctca cgcggtactt caccgcgagg
     9061 agcgcctcgg cgtacttggt cgcgatgccg aagacgcccg tgagccagca ccagaggacc
     9121 gcgcccggcc cgcccatcgc cacggccgtg gccacgccca cgatattgcc cgtgccgatc
     9181 gtggccgcga gcgccgtggt gagcgacccg aaatggctca cgtcgccgtg cccgtcgtct
     9241 tccttttgga acgaaagccg gatggccagg aacacgtacc gctggatgcc gcgcaggcgc
     9301 accgtgagaa acaggtgcgt gccgaagagg acgacgagca tcggcggtcc ccagaggtag
     9361 ccgtttatga gcttgatgat gccgtgaatg gtgtccatgg gcgcctcgcg gtgcgtgata
     9421 tcgagcgctg acgctagtga tgagcgagtg cgttgtcaag aaaatattac ggaacaaata
     9481 tgttcgatgg cgcgtcaatt accacacttt tgtcgcgaga ggcgcgcgat tgattgcgcg
     9541 tgatgccgtt tcagcagccg tgtgcgtcgc tctgcgcgat cgctgtgcag cgcgcgcggc
     9601 gctgcgcgca tgacactgat gcgtcgcgga gtgctatcat cccccccgca cggaatattt
     9661 aaccgttccg taacatcgag tgcgccgtac gccctgcatg tggcacggtt tatgcagtat
     9721 agagtggcat gacgtaaaaa cgtttggtat ggaggtagta tatgaaaaaa agattcgctc
     9781 ttatcgcatt cctgctcatc gggatgatcg cgctcgcgcc gctgggcgcg caggaggccg
     9841 cgaaggccgc cgcgcccaag gagctgcatt cgtcgggcga tttcatctgg atcatgattg
     9901 cggcgttcct ggtcttcttt atgcaggcgg gcttcgccat ggtcgagacc ggatttacgc
     9961 gcgcgaagaa cgcgggcaac atcatgatga aaaacctgtt cgacttctcg atggggtcca
    10021 tcgcgttttt cgtcgttggc tacggtctca tgttcggcgc gaccaagctg gggctcatcg
    10081 gatcgtcggg atttttcctg gccgacgcct cgcccaagcc ggacgccgac ggcgggaacg
    10141 ccggcctgtg gcagtacgcc tactttctgt ttcagctcgt cttcgccgcc accgcggcca
    10201 ccatcgtctc gggcgccatg gccgagcgca ccaagttcgt gagctatctc gcctacagcc
    10261 tggtcatctc ggcgctcatc tacccggtgt cggggcactg gatctggaac gcgaacggct
    10321 ggctcgcgaa actcggcttc caggatttcg ccggttccac cgtggtgcac atggtgggcg
    10381 gcgtcgcggc catgatgggc gcgctggtgc tgggaccgcg gtatggaaaa tacggacccg
    10441 acggcaagcc gcgcgccatt ccggggcaca atatcccgct ggcggcgctg ggcgtgttca
    10501 tcctgtggtt cgggtggttc ggcttcaatc ccggcagcac cacggcggcg acgccctcga
    10561 tcgcatttat cgcgatcaac actaatctcg cggcggcggc aggcgcgctc gcctcgatgg
    10621 cggccatctg gatccagacc ggcaagcccg acgtgagcat gagcttgaac ggcgcgctcg
    10681 cggggctggt ggccatcacc gccgggtgta atttcgtctc gccggcaagc tccatcgtca
    10741 tcggcctcat cgccggcgtc gtggtggtgc tcgcggtgca gctgttcgat ttcgtcttca
    10801 agatcgacga tccggtgggc gcgatctccg tgcacggcgt ctgcggggcg ctcggcacca
    10861 tcctggtcgg cgtcttcggc gacgcgacct atggcggcat gaacggactc atcaacggcg
    10921 gcggattcaa gctgctgggc atccaggcgc tgggggcgct cgtggtgttc ggctgggtgg
    10981 ccgtgacggc cgggattttg ttcctggtca tcaagaagac catcggcctc cgggtgagcc
    11041 gcgaggagga gctcaagggc ctcgacatcg aggagcacgg catggaatcc tactccggat
    11101 tccagatttt caggaaccag tagggaggcg cgaccatgaa gctcatcact gcatacattc
    11161 aaccggaaaa gctcaacgac gtgaaacagg agctcttcaa ggaacagatc agcaagatgt
    11221 ccgtgaccaa ctcgctgggc tgcgggcagc agggcggcta caccgagacc tatcgcggcg
    11281 tgcccatgga ggtgaacctc cttcacaagg tgcgcatcga gatcgcggtg aacgacgatt
    11341 acgtcgatcg cacggtgaag gcgatcatcc gcggcgcgaa gaccgggaag atcggcgacg
    11401 ggaagatctt cgtcgtgaag ctcgaaaact gcatccgcat ccgcactgag gaagagggga
    11461 aagaggccat cggctgatcg acgcttgaat catcaacctc atgtaaagcg cgtatccctc
    11521 gggcccacgg gtccggggga tttttttagt acgccggatc gtgcgccgag ggcggttcgc
    11581 ggagggcgct atcgcgttag acgatctcga atccgatgat caccaggtcg tcctcgatgg
    11641 gcgcgaagcc gcggaactcg cgcacgtggt cgatgatgcg gtccagcgtc tcgcccaggc
    11701 ccggcgccgt gagccgctcg aagagcgccg ggaggtcgtc gtagccgatg atctcgtacc
    11761 gttcgttgcg cgtctcgggc aggccgtcgg tgaagaggaa gacgcggtcg cccgtggcga
    11821 gatcgacgcg ctcgtcggtg aactcgaagt cgtcgaattt tccgatgatg gtcccgcgcg
    11881 atttgatcat ctcgaccgat ccggtggcgg cgcgatagag cagcgggtgc gggtgccccc
    11941 cgcgcgaaaa ggtgaagcgg aagcgcgatc ggccgatctc ctggaagtag ccgtagatgg
    12001 cggtgaggaa gttgttggcc atggtgtcgg cgagatcgcg gttgagcgtg cggatgaact
    12061 cgagcgggtt ttgcccgtgc gcgcggcaga tgcggtcggc ggtcgccttg accagggtga
    12121 tgaacagcgc cgccgacacc ccgtggcccg agatgtcgcc gatgaaaacc ccgaggcccc
    12181 cctcctgcag cgtggtgaag gagaaataat cgccgcccac cgcctcgagg gacgcattgc
    12241 ggaaatcgca ggcgatgccc gggtagcgcg gcggggcgct cggcaagagc tcctggtgga
    12301 tgagctgcgc gttcttgatg tcgctttcga tgatctcgtt gcgctcgcgc agctcctcct
    12361 cgttcgcgcg caacgcctcc tcggccaggc ggcgattgta ggattccagc gccagcgagg
    12421 cgaagtccga gagcgatccg gcgaagttct cctcgtcggg cgtccagagg cgcggcggcc
    12481 ccacgtgctc cagcgagagg atgcccgagg tcatgccgcc caggcggaag ggcaccgcga
    12541 gcagcgcgcc gatcccgaga cgggcgagat ggcgcgcgac gatcgcgcgg gtccgctcgt
    12601 cggcggccgc gtcggtcgcg tcgatgagcc ggtcgcgttc gatcgcgtcg aagaattcgg
    12661 ggaagtcctt cacggcgatt tcccacccgc tgcgatggct gtcgtgcgtc gcggcgaagc
    12721 tgtcgtagca gatgatccgg gtgcggttgc ggtcgaagag ccagatgctg cttcgctcga
    12781 tgtccaggtt gtgcgctccg ctggtggtga tggcgcggaa ggagcccttc aggtctccgg
    12841 cgtagagcga ctcggtccgc gcgagcgtcg ccagcgccat gtgctgcagt cggaggcgga
    12901 tctcgttttg ccagagcttc tcctccgcgg cgcgccgctc ggtgatgtcg cgcgcgatcc
    12961 cctggaagcc cacgatggcg tcgtccttga agatggcctg cacgttttgc ccgatccagt
    13021 agacggtgcc gtcgcgcgcc acgaccgggt gctcgttgta ggtcgacggg atgcgcccca
    13081 ggtactgggc gcgataaaac tcgatcacct cgtcgcggta gtcgggatgc acgaactcgc
    13141 tgaagtggcg ccccaggatc tcgcgctcct cgcactgcat gcgggccagc gccgtcgggt
    13201 tgagatacgt gaagaagccc agcgcgtcga agctgatgat cacgtcgccg gcgtgggtca
    13261 cgagctgctt gtagcgctcc tcgctctcgc gcaggcgcgc ctcgaagcgc ttgcgctccg
    13321 ttatgtcgcg cacaacgccg cggaaccccc gcggcgcgcc gtcgtggtcg gtgatcagcg
    13381 agaccgacgt ttccgccgag atgcgttcgc cgtcgcgccg gatgagctcc cagtcgaagg
    13441 agcgcacggg ccggccctcg cggaacaccc ggttgaagac ggtgaagatg cgtcgcgagt
    13501 tctcgccgtc catgtaggcg cggtagttga ggccgatgag cgcgcccggc tcgtagccga
    13561 gcatccggta catcgagccg ttgcagaagg tgaaatcgcc cttgaggtcc acctcgtagt
    13621 agccgtcctc gatcgtctcc acgatggtgc gatagcgctc ctcgctcgcg gagagcgcct
    13681 tctccgctgc cttgcgatcg tcgatctccc gactggccac caggacgctc cgggccccgg
    13741 attcgttcgt catcacggtc ccgtcgcatt ccagccaggc gtacgtcccg tccgcacgcc
    13801 ggaagcgcag ctcgatgcgg ccaccgcgac cgtccgagaa accgtcgcgg aacatcgcga
    13861 gcgcggcggg aacgtcgtcg gggtgcacca ggtcgaaggc cgacatcccc atgagg
//
LOCUS       DAORHF010000084        13640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3563_length_13640_cov_4.811042, whole genome shotgun sequence.
ACCESSION   DAORHF010000084 DAORHF010000000
VERSION     DAORHF010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3563_length_13640_cov_4.811042"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKJ16_08700"
     CDS             complement(<1..367)
                     /locus_tag="PKJ16_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HNV47107.1"
                     /translation="MGPKDYLFTSESVSEGHPDKVADQVSDAVLDAHLAGDKRSRVAC
                     ETLVTTDRCIVSGEITSTQKVDYEKVARKTIGDIGYTHADIGFDAKTCKVEIYVHSQS
                     PDISQGVTAGEGLFKEQGAG"
     gene            complement(465..653)
                     /locus_tag="PKJ16_08705"
     CDS             complement(465..653)
                     /locus_tag="PKJ16_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HNV47108.1"
                     /translation="MAKTVIVNRDECTGCELCADTLPEVFEMDADGLCRIKDPTGASE
                     DKIQEVIDSCPAECLHWK"
     gene            complement(769..1161)
                     /gene="secG"
                     /locus_tag="PKJ16_08710"
     CDS             complement(769..1161)
                     /gene="secG"
                     /locus_tag="PKJ16_08710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HNV47109.1"
                     /translation="MSILISVLTVVFVILSGLLIVIILLQSDKSSGMGILGGSSQSTF
                     GSSTADIITKITTAMVALFMIGSLGLSILESIRDRAIERDLVGDKKPSGVIGPGVQDG
                     KKEPVDAKKDATAPKTAPAQPVQQAPAK"
     gene            complement(1211..1966)
                     /gene="tpiA"
                     /locus_tag="PKJ16_08715"
     CDS             complement(1211..1966)
                     /gene="tpiA"
                     /locus_tag="PKJ16_08715"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HNV47110.1"
                     /translation="MKRREIFAGNWKMYKDYNGAVALVNGLKGGLGDIGAREVAIFPP
                     ALYVREMVSLCTGTRIDVGVQNMYFEKEGAFTGELAPAMVKDCGARYILIGHSERRHV
                     FRETDVEVNKKVAAALAVGLDPMVCVGELLAEREAGTSEAVVKTQLVEALKGVTKERM
                     AKVVVAYEPVWAIGTGKVATPEIAETMHRFIRETLGALYDGATAAMVPVLYGGSVKPD
                     NIAGLYAMENIDGCLVGGASLASDSFLDIIKVK"
     gene            complement(2039..3049)
                     /gene="gap"
                     /locus_tag="PKJ16_08720"
     CDS             complement(2039..3049)
                     /gene="gap"
                     /locus_tag="PKJ16_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HNV47111.1"
                     /translation="MGIKVGINGFGRIGRNVLRILLQNPDKFKDVEVVGINDLTNAAM
                     LAHLFKYDSVHRIFDGTVSSKEGAIVINGKEIKVHSQKDAKALPWKADGVDVVVEATG
                     RYRDKEGCMAHIDAGAKKVIITAPSKNEDLTVVLGVNEDKYDAAKHVVLSNASCTTNC
                     LAPVAKVINDSFGIERGLMTTIHSYTNDQRILDLPHEDFRRARAAALSMIPTTTGAAK
                     AVGLVIPELKGKVDGLAIRVPTPNVSVVDFVCSIGKEASKDDVIGALKSAAAGKMKGI
                     LDVCDTELVSVDFLGNSFSSIVDVPASSVIDKRLVKIISWYDNEWGYSFRVCDLIKFI
                     MK"
     gene            complement(3194..4216)
                     /locus_tag="PKJ16_08725"
     CDS             complement(3194..4216)
                     /locus_tag="PKJ16_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV47112.1"
                     /translation="MANKPAEYCSRCGAKLERVMLDDRPRERCASCGAVVYVNPLPVV
                     SSILPNDRREILLVLRKKEPYSNQWCLPMGFAEIDENIQDAALRELEEETSVKGRIIR
                     LLDAETTDDSFYGNLAILCYEVEHVSGEVRAGDDALDARFFPIDEVPRLAFPPNDRAV
                     EKFRELYRDLWKIQDSFAAMFPKGGRVDIDMHSAVGVEGLLSDLVMGTLEQKIDGIII
                     FWLEYLKAKDTAKRYLALPPKKIYEDGAFILTGLTRWMRDKRFAPDDLHSFVSLGSGY
                     CAGGIPAADVMSAYTLFRKAIWQFILGQGFHISFLELYRSLEVNNRVVLFFDKAIYHM
                     LRGYEK"
     gene            complement(4458..4901)
                     /locus_tag="PKJ16_08730"
     CDS             complement(4458..4901)
                     /locus_tag="PKJ16_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47113.1"
                     /translation="MKNFITVAVIVACAFAFTACGQTIGGQIKGDKFVTEGWVDDNTF
                     RTTATGVPKKGLTNKTQRRLTAREAALLYAQKTVLEKFKGARIEGASGMADGASTGIA
                     IAKEFGGLVRGGSVQKETYNDGDECEVVYEITAPGLKKRVIGGAE"
     gene            complement(5010..5705)
                     /locus_tag="PKJ16_08735"
     CDS             complement(5010..5705)
                     /locus_tag="PKJ16_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47114.1"
                     /translation="MKRFLPVLVFVLAGCVTLPVHLKYPSKAGNSVLGVAITLRAPIA
                     IFTDYPTAVYFAKIEKGETHLTAKRVIPANFVARGRAYLVDCEPGEYVAVAAYTRKQA
                     FKHQKDFSKPDMSPGEEQFYCTYFGEKVIGASRVSVGKNEFVFMGDIVVDLSILILNT
                     EKVDMAIANYLGKVKFPDSPFLYLGDLKNIDRGPKIMVGLYRAAAKEFAESGWSPLVN
                     RRLMEPAGGQKAR"
     gene            complement(5766..6353)
                     /locus_tag="PKJ16_08740"
     CDS             complement(5766..6353)
                     /locus_tag="PKJ16_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47115.1"
                     /translation="MKRSIIVLLCSVPVFFSCVGFFMISKNQSAPKPGRAIIYGLMIE
                     MPGFGNSFSIDLYDRKNDSTYQLAIKSNWAPETSLPFAFELPAGTYELFSFTVGQASG
                     QKASYQTYYLVKNASELKKLTGTYVGNKYLAFKTLEHDSIQLQPDKVCYVGEWAFSDL
                     KPVITAKKELTDRIMKESFPGLMTATAVNAVPGAK"
     gene            6634..7608
                     /locus_tag="PKJ16_08745"
     CDS             6634..7608
                     /locus_tag="PKJ16_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020061312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HNV47116.1"
                     /translation="MRSFARPIIAVSACLEFEACRYNALSVACPLVRDLMPFVDFRPA
                     CPEKAIGLGVPRDPIRLVRDGDAVALYQPATGRDVTALMDDFTQRHLATLRDADGFIL
                     KGRSPSCGIKDVKVYPDRDPAKTPITGKAAGLYGGAVIAAFPGRAIEDEGRLENFTIR
                     EHFLTRIFALAEFRGVYASGSMARLVQFQADNKLLLMAYNQTEMRALGRITANPGGRP
                     WRETIDEYETHLRAALSRVPRFQSNINVLQHAMGYFSEGMSASEKRFLLGEFETYRAG
                     KIPLSAVTGIIRALIARFGSDYLSRQTYFAPYPTELVSITDSGKGRGA"
     gene            complement(7626..8483)
                     /locus_tag="PKJ16_08750"
     CDS             complement(7626..8483)
                     /locus_tag="PKJ16_08750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV47117.1"
                     /translation="MDKAEYSEKEIRGTFGKPADHVSVHKTIVSFSRARADIRAVALG
                     GLDLSGVRTVLDLGCGFGFFSMGLSGLIPRNALVLGVDMLEENRDPYLHNTAEAGLAT
                     EFLCAPSQAIEEFEPQTYDLISASYSLYFFPELVPAIARILRPMGIFVTLTHSRESLR
                     EIIDLIPECYRRLGVTIERDRIHIKRLLESFCAENGADLLAPHFGSVETIGWENSLEF
                     PLDGMKHLYGYIINKKYLLLKELMDQKPQLEQNAVEMLFAMAHRISVERGGFRLNKND
                     AIFRCGRPR"
     gene            8533..9114
                     /locus_tag="PKJ16_08755"
     CDS             8533..9114
                     /locus_tag="PKJ16_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47118.1"
                     /translation="MKSITSKMRIFRDEILKNKVFISKTGLALRITEKDFRMEIDMPI
                     DTTLYVHQTTLQLIEAACVMTGIRRGQLVVLLLARAILNRGKALKSGSATRYQTRDRR
                     EMWRTIHVRMLADDADYFRDARCFFNESVSLILAQAAREYLREILQRSRSGEKVDNYP
                     LKNYIICREERDGLIFWHICWGFPEKPEKYFRL"
     gene            complement(9234..9881)
                     /locus_tag="PKJ16_08760"
     CDS             complement(9234..9881)
                     /locus_tag="PKJ16_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HNV47119.1"
                     /translation="MPLVEKIKFKGDGVMISVDTGEALMVSAAVFAQYRLAAGTEIDA
                     AAYRQLRDESDRYMCRRAALSYLAVRAHSAHEMESYLRRKGYSGDSIAETVRDLCERH
                     YIDDLAFATEYAGARKRRGGVGRNLVTRELQRKGVSRDIIATAISRATSEDDEREAAL
                     ACARKKLRQRAGKKNRASRLAFYLQGRGFDAAVIRAVLRELGPLDDEEDAGAAEE"
     gene            complement(9892..10287)
                     /locus_tag="PKJ16_08765"
     CDS             complement(9892..10287)
                     /locus_tag="PKJ16_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47120.1"
                     /translation="MQGIFRTWLLMMTLALVLSMAGCGAHGFSELKRRLHAETDRIEI
                     RRGSRVVAVTDPVTVRLVVDGVASESAPLYKCAYDGSIQFFCRGVALLPEPMEFNLSP
                     ACLHVKFVYRDSLHSKRISPGLAGFLRGL"
     gene            complement(10390..10782)
                     /gene="rpsI"
                     /locus_tag="PKJ16_08770"
     CDS             complement(10390..10782)
                     /gene="rpsI"
                     /locus_tag="PKJ16_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HNV47121.1"
                     /translation="MAAERYYATGRRKTSVARVWLKPGSGKILVNEQDVSEFFRRKTL
                     ELIVRQPLELLSAVSAFDIEATTLGGGWSGQAGAVRMGIARCLIQHDERNKKALREAG
                     FLTRDSRQVERKKYGRKKARKRFQFSKR"
     gene            complement(10810..11256)
                     /gene="rplM"
                     /locus_tag="PKJ16_08775"
     CDS             complement(10810..11256)
                     /gene="rplM"
                     /locus_tag="PKJ16_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HNV47122.1"
                     /translation="MNSSQRTFSIKASQIEKKWLLVDADGAVLGRLATEVAHLLRGKH
                     KTTYTQHMDDGDNVIVINADKVVLTGSKGDDKEYFSHSHFPGGVKFTNIKKYKADNPE
                     FIIEHAVKGMLPKNRLGRKMFKNLKVYAGTAHPHQAQKPETYDIKK"
     gene            11516..12136
                     /locus_tag="PKJ16_08780"
     CDS             11516..12136
                     /locus_tag="PKJ16_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47123.1"
                     /translation="MSPKIVDVDEKKKQITDAAIRVLARKGLKETKMEDVAEAAGIGK
                     GTLYEYFKSKDELFLVAYRHIMEEFECQTDLKLPENVTTIDTLKAIISMMTSMHTSDF
                     PLDHFQVLTEFWLEEVKSANQGKKRQFDMLATYREYIDLVKAVFEQGIQKGEIRDINP
                     EHAATILMMIFDGLMLLWMLDKNSFDFKGTVDTFGGIFIKGIQKQG"
     gene            12324..13079
                     /locus_tag="PKJ16_08785"
     CDS             12324..13079
                     /locus_tag="PKJ16_08785"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV47124.1"
                     /translation="MKSLMIILALITALASTALPSYALTGKEIIRKTEDAMRGTSVVG
                     TYLITVKTKLWTRTMKLKAYDQRAGKKSFNEIYEPKKDAGNRFLMINKNMWHYVPKLQ
                     QTIKISPSMMLQSWMGSDFTNDDIVKESSIEEDYTHTVTGTATVDGHPCHVILLTPKP
                     DAAVVWGKIIYSVRQSDYLPVREEFYNEHNVLKRVMTFGQFKKMDDRVIPTLWKMQTV
                     SKPEAHTILEIASIRFNTAIPSRTFTMQNLTRK"
     gene            13082..>13640
                     /locus_tag="PKJ16_08790"
     CDS             13082..>13640
                     /locus_tag="PKJ16_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47125.1"
                     /translation="MNREAIAQLAWRNVWRNKRRTILTLATIVVGCGMIILNNALAKG
                     GHDGMINDAAITSAGHIQVHRAGYWASRTIDYAFQVPDGLTAALGRDRRIAAHTERIH
                     AAGLLAFGANTSGGQIQGVDPAREPAVTNLHTWVLPGGRYLTAEDSTHAIVGETLAKN
                     LGAKVGDVVSIISQGLDGSIAAEKLT"
BASE COUNT         2830 a         4156 c         3928 g         2726 t
ORIGIN      
        1 cgcccgcgcc ctgctccttg aacaacccct ctcccgcagt gacgccctgg gagatgtccg
       61 gggactgcga gtggacgtag atctcgacct tgcaggtctt cgcatcgaag ccgatgtcgg
      121 cgtgcgtata gccgatgtcg ccgatggttt tccgggcgac cttctcgtaa tcgaccttct
      181 gggtcgaggt gatctcgccg gagacgatgc agcgatccgt cgtcacgagc gtttcgcacg
      241 cgacgcgcga acgcttgtcg ccggccagat gcgcatcgag cacggcatcg gatacctggt
      301 cggccacctt gtcgggatgg ccttcggaca ccgactcgga ggtgaataaa tagtctttgg
      361 ggcccattgg gaactccttg ataatgaaat gaggtgcgac tggcgcgccc acaggctatg
      421 acagcggcac cgtccgttga aatgcgattt tttttgcgtg aacgctattt ccagtgaagg
      481 cactcggccg gacagctgtc gatcacttcc tggatcttgt cttcgctcgc gccggtgggg
      541 tccttgatgc gacagaggcc gtcggcatcc atctcgaaga cctccgggag cgtgtcggcg
      601 cacagctcgc agccggtgca ttcatcgcga ttcacgataa ccgttttcgc catggcgtat
      661 ctcctcatca ttattgtaga tcgccgggag agtggcaccg gcgttcgccc tgccccgttc
      721 ggcatcagcc gtgcgagggg gaacgggcgt actggcgcag gaattggatt acttggctgg
      781 cgcctgctgc accggttgcg ccggcgccgt cttcggtgcg gtcgcgtcct ttttcgcgtc
      841 gaccggttct tttttaccgt cctgaacgcc cggtccgata acgccgctcg gctttttgtc
      901 gccgaccaga tcccgctcaa tggccctatc gcggatcgat tccaggatcg acagccccag
      961 ggagccgatc ataaacaggg cgaccatggc cgtcgtgatc ttggtgatga tgtcggcggt
     1021 cgacgatccg aaggtggatt ggctcgaccc gccgagaatc cccatccccg agctcttgtc
     1081 cgattggagc aggatgatga cgatcaacaa accgctcaga atgacgaaaa cgacggtgag
     1141 gacggaaata aggatgctca ttgacaaatc tcccgggcgt tctgcggcgc gtcgcgcacc
     1201 gtgccattaa ttatttcact tttattatgt caagaaaaga atcgctcgca aggctcgccc
     1261 cgcccacgag acatccatcg atgttttcca tggcatagag cccggcgata ttgtccggct
     1321 tgaccgaccc gccgtagaga acgggaacca tggccgcggt ggcgccatcg tagagcgcgc
     1381 ccagcgtttc acggataaac cgatgcatgg tctccgcgat ctccggcgtc gccaccttgc
     1441 cggttccgat cgcccagacg ggttcgtagg cgaccaccac cttcgccatc cgctctttcg
     1501 tgacgccctt gagcgcctcg acgagttgag tcttcaccac cgcctcggac gtgccggcct
     1561 cgcgctcggc gaggagctcg ccaacgcaca ccatcgggtc gaggcccacg gcgagggccg
     1621 cagcgacctt cttgttcacc tcgacatcgg tctcgcgaaa gacatggcgc cgctccgagt
     1681 ggccgattag gatgtagcgc gcgccgcagt ccttcaccat cgccggcgcg agctcgccgg
     1741 taaaggcgcc ctccttctcg aagtacatgt tctgcacgcc cacgtcgatg cgggttcccg
     1801 tgcagaggga caccatttcg cgcacgtaca gcgcaggcgg aaagatggcg acctcgcgcg
     1861 ccccaatgtc gcccaggccc cccttgaggc cgttcaccag cgcgacggcg ccattgtaat
     1921 ccttgtacat cttccagttg ccggcaaaaa tctcgcgacg cttcattgtg atgtcctttt
     1981 acgtgcaaag tttccccctc cccctatcga agggggaggt cgggagcggg tcgaacccct
     2041 acttcatgat gaacttgatc aagtcgcaca cgcggaacga gtagccccac tcgttgtcgt
     2101 accagctgat gatcttgacc aggcgcttgt cgatgaccga cgaggccggg acatcgacga
     2161 tcgacgagaa cgaattgccg aggaaatcga ccgatacgag ctcggtgtcg cacacgtcga
     2221 ggatcccctt catcttgccc gcggcggcgg acttgagcgc gccgatcacg tcgtccttcg
     2281 aggcctcctt tccgatggag cacacgaaat cgacgaccga aacgttcggg gtggggacgc
     2341 ggatggcaag accgtccacc ttgcccttga gctcggggat caccaggccc acggccttcg
     2401 cggcgccggt ggtggtgggg atcatcgaga gcgcggccgc gcgcgcgcga cggaagtcct
     2461 cgtgcgggag gtccaggatg cgctggtcgt tggtgtacga gtggatggtg gtcatgaggc
     2521 cgcgctcgat gccgaaggag tcgttgatga ccttcgcgac cggcgcgagg cagttcgtgg
     2581 tgcacgaggc gttcgacagg accacgtgct tggccgcatc atacttgtcc tcgttcacgc
     2641 ccaacaccac cgtgaggtcc tcgttcttcg acggggcggt gatgatgacc ttcttcgcgc
     2701 cggcgtcgat gtgcgccatg caaccttcct tgtcgcgata gcggccggtg gcctccacga
     2761 ccacgtccac gccatcggcc ttccacggga gcgccttcgc gtccttctgc gaatggacct
     2821 tgatctcctt gccgttgatg acgatcgcgc cctccttgct gctcaccgtg ccgtcgaaga
     2881 tccggtgcac ggaatcgtac ttgaacaggt gcgcgagcat ggccgcgttg gtgagatcgt
     2941 tgatgcccac gacctcgaca tccttgaatt tgtccggatt ttgcaggagg atccggagaa
     3001 cattccgtcc gatacgcccg aagccgttaa taccgacctt gatacccatg gtgctgcccc
     3061 ctcttttcgt tgtagattag tagaatactc gcgttgtgat tgccgccgaa gaccttcgcc
     3121 ctctggtacg gccgcgttcg gcgcgcggtg actgatagtg cgctccgcat ggcggtattt
     3181 ttcaagaaaa aatttatttt tcgtacccgc gaagcatgtg atagatcgct ttatcaaaaa
     3241 agagcactac acgattgttt acctcgaggc tccggtacag ctcgagaaag gatatgtgga
     3301 atccctgtcc gagaatgaac tgccagatcg ccttgcggaa gagcgtgtag gcgctcatca
     3361 catcggccgc gggaatgccg cccgcgcaat agccgctccc gagtgacacg aacgagtgca
     3421 ggtcgtcggg cgcgaaacgc ttgtcgcgca tccatcgggt gaggccggtg agaatgaacg
     3481 cgccgtcttc atagatcttt ttcgggggca gcgcgagata gcgtttcgcg gtgtccttcg
     3541 ccttgaggta ctccagccag aagatgatga tcccgtcgat cttctgctcg agcgtgccca
     3601 tcaccaggtc ggagaggagc ccctccacgc caacggcgct gtgcatgtcg atatcgaccc
     3661 gcccgccctt cgggaacatg gcggcgaagg aatcctgaat cttccagaga tcgcggtaga
     3721 gctcgcggaa tttctccacc gcgcggtcgt tcgggggaaa cgccaaccgg ggcacctcgt
     3781 cgatcgggaa aaaccgcgca tccagcgcat cgtcccccgc gcggacctcc ccggaaacat
     3841 gctccacctc gtagcagaga atggccaggt tgccatagaa ggaatcgtcg gtcgtctccg
     3901 catcaaggag ccgtatgatc cggcccttca cgctggtctc ctcctcgagt tcgcgcaggg
     3961 ccgcgtcctg tatgttctcg tcgatttcgg cgaagcccat cggcagacac cactggttgc
     4021 tgtacggctc cttcttgcgc agcaccagca ggatctcgcg gcggtcattg ggcaggatgg
     4081 agctcaccac cggaagcgga ttcacataga cgacggcgcc gcaggaggcg catcgttcgc
     4141 gcgggcgatc gtcgagcatc acccgctcga gcttcgcgcc gcagcgcgag cagtattccg
     4201 ccggtttgtt cgccatgagc acctcccgat atgcgcccgg atcatcgcca caatacacgt
     4261 cgcgaccgcc actggcttac aaagtaccaa caatcgccat cggcggcaag gaaaggaaga
     4321 aaaaaacgcg cctggcggta cactccgatg ggtgatgttt cactcgagtg agccgaaggg
     4381 ccatcacgag gggggcagac gcgaaacggc cgtatccatc gacacggccg tttgcgcgcg
     4441 actgaacagt atggaaatta ctccgcgccg ccgatcaccc gcttcttgag tccgggagcg
     4501 gtgatctcat agacaacctc gcactcgtcg ccgtcgttgt aggtttcctt ttggaccgat
     4561 ccgccgcgca cgagcccgcc gaattccttc gcgatcgcga tgccggtgga ggcgccgtcg
     4621 gccatgccgg acgcgccctc gatccgcgcg cccttgaatt tctcgagcac ggtcttctgg
     4681 gcgtagagca gcgccgcctc gcgcgcggtg agccgccgct gagtcttgtt cgtgaggccc
     4741 ttcttgggaa cgccggtcgc ggtggtccgg aaggtgttgt cgtcgaccca cccctcggtc
     4801 acgaacttgt cgcccttgat ctggccgccg atggtctgac cgcatgcggt aaacgcgaat
     4861 gcgcacgcca cgatcacggc tacggtaatg aagttcttca ttttactcct ccctgatgca
     4921 tggtaactgt aatcagcccg ggtgatgctc ccgccggact gtaccgtatc tgacgacgag
     4981 ggccgttctt ttgggaaaaa aaagtgctgc taacgagcct tctgcccgcc cgcaggctcc
     5041 atcaatcgcc tgttgacgag gggcgaccag cccgattccg cgaattcctt ggcggctgcg
     5101 cgatacaacc ctaccatgat tttcggcccg cgatcgatgt tcttcaggtc gccaaggtag
     5161 aggaatgggc tgtcggggaa tttgaccttg cccaggtaat tggcgatcgc catgtcgacc
     5221 ttttcggtat tcagaatcag tatggagagg tccacgacga tatcgcccat gaagacgaac
     5281 tcatttttgc cgaccgaaac ccgggaggcg ccaataacct tttccccgaa ataagtgcag
     5341 taaaactgtt cctcccccgg gctcatgtcg ggcttgctga aatccttctg gtgcttgaac
     5401 gcctgcttcc tcgtatacgc ggcgacggcg acatactccc ccggttcgca atcgacaaga
     5461 taggctcgtc cgcgcgccac gaagttcgct gggatgactc gcttcgcggt gagatgcgtt
     5521 tcacccttct cgattttggc aaaatacacc gcggtcggat aatcggtaaa tatggcgatt
     5581 ggcgcgcgca gggtgatggc aacgccgaga acggaatttc ccgccttcga tggatacttc
     5641 aggtgtacgg gcagcgtgac gcatccggcg agaacgaaca cgagtaccgg cagaaatctt
     5701 ttcatttcgt tgacacctcc atggtcaggt cgatctcggt tattccgtgt cgttgttcaa
     5761 agtcgttatt tcgccccggg aaccgcgttg actgcggtcg cggtcatcaa accggggaat
     5821 gactccttca taatcctgtc ggtgagttcc tttttcgccg tgattaccgg cttaagatct
     5881 gaaaacgccc actcgcccac ataacagact ttgtcgggtt gcagctgtat gctgtcgtgt
     5941 tccagcgttt taaaggcgag atatttattg cccacatatg tccccgtcag ctttttcaac
     6001 tcactggcgt ttttgaccaa atagtacgtc tggtaggatg ctttttgacc cgacgcctga
     6061 cccaccgtaa aactgaacag ctcataggtt cccgccggca gctcaaacgc aaacggcagg
     6121 ctcgtctcgg gagcccaatt gctttttatc gccagttgat acgttgaatc attttttcgg
     6181 tcgtataaat cgatgctaaa cgagttcccg aatccgggca tctcgatcat aaggccgtag
     6241 ataatcgctc gcccgggttt cggcgcggat tggttttttg aaatcatgaa aaaaccgaca
     6301 cacgagaaaa agaccggcac cgagcacaga aggactatga tacttcgttt catgaagcct
     6361 ccgaacggtc gttcattgat agctacaatc aaaaaaaatc gaaaaacgct cgccgcctgc
     6421 gagctccccg cgggacatcg ttcacgccac attcctctcg cagaaagcat acgaagcggc
     6481 accatgaaag gtcaaggctt tatctgccgg caagggagat tgcgcgactc ggacatggcg
     6541 ggcgcctcat gccgccggcg accccatagt gcttgacgga ctgattgcgc gatagtatcg
     6601 tcgccctatg aattcacgcc catcaggccg cgcatgagga gcttcgcccg gcccatcatc
     6661 gccgtgagcg cctgcctcga attcgaggcg tgccgctaca atgcgctcag cgtcgcctgc
     6721 ccgctggtgc gcgacctcat gcccttcgtc gattttcggc cggcctgtcc cgaaaaggcc
     6781 atcggcctgg gggtgccgcg cgatcccatc aggcttgtcc gcgacggcga cgccgtggcg
     6841 ctctatcagc cggcgaccgg gcgcgacgtg acggcgctca tggacgactt cacgcagcga
     6901 catctcgcga cgctgcgcga tgcggacgga ttcatcctga aaggacggtc cccgtcctgc
     6961 ggcatcaagg acgtgaaggt gtacccggac cgcgacccgg cgaaaacacc gatcaccgga
     7021 aaggcggcgg gactgtacgg cggagcggtg attgcggcgt ttcccggccg cgcgatcgag
     7081 gacgaggggc gtctcgagaa cttcaccatc cgcgagcact tcctcacgcg catcttcgcc
     7141 ctcgcggaat tccggggcgt gtacgcatcg ggctcgatgg cgcgcctggt gcaattccag
     7201 gccgacaaca agctcctcct gatggcctac aatcagacgg agatgcgcgc gctggggcgg
     7261 atcaccgcga acccgggcgg acgcccatgg cgtgaaacga tcgacgagta cgagacgcat
     7321 ctgcgcgcgg cgctgtcacg ggtcccgcgc ttccagtcca atatcaacgt cctgcagcac
     7381 gcgatggggt atttctcgga ggggatgtcc gcgtccgaga agcgcttcct tctcggagag
     7441 ttcgagacct atcgggccgg caagatcccc ttaagcgccg tgaccggcat catccgcgct
     7501 ctcatcgcgc gcttcgggag cgactacctc tcgcgacaga cctactttgc gccctacccg
     7561 accgagctcg tctcgattac cgactccggc aagggccgcg gcgcctgacc gattttgcat
     7621 ttcgactaac gcggccggcc gcaacggaat atcgcgtcgt ttttattgag acgaaagccg
     7681 ccgcgctcga ccgagatgcg gtgcgccatc gcaaagagca tctccaccgc gttttgctcc
     7741 agctgcggct tctggtccat gagctccttg agcagcaagt acttcttgtt tatgatatac
     7801 ccatagagat gcttcatgcc gtcaaggggg aactcgaggc tgttctccca gccgatcgtc
     7861 tccacggatc cgaaatgcgg cgcgaggaga tcggcgccgt tttccgcaca gaacgactcc
     7921 agcagacgct tgatgtggat ccggtcgcgc tcgatggtca cgccgaggcg acggtaacac
     7981 tccggaatga gatcgatgat ctcgcgcagg ctctcgcggc tgtgcgtgag cgttacgaag
     8041 atccccatgg gccgcaggat tcgtgcgatc gcggggacga gctccggaaa aaaatacagc
     8101 gagtagctcg cggaaatgag atcgtacgtc tgcggctcga attcctcgat cgcctgcgac
     8161 ggggcgcaca gaaactcggt cgcgaggccc gcctccgcgg tattgtgcag gtaggggtcc
     8221 cggttctcct ccagcatgtc gacacccagc acgagcgcgt tgcgcgggat cagccccgaa
     8281 agccccatgc tgaaaaaccc gaacccgcac ccaagatcaa ggacagtgcg aacacccgag
     8341 aggtcgagac cgcctaacgc gacggcgcgg atatcggccc gagcgcgtga aaagctcacg
     8401 atggtcttgt gcaccgagac gtggtccgca ggctttccga acgtgccccg aatctccttt
     8461 tcggagtatt ccgccttatc catgccatgc ctttcagaga acaaaaattc aacaggatga
     8521 agatacggcc tgatgaaatc aattacaagt aaaatgcgaa ttttcaggga cgaaatcctg
     8581 aaaaataaag tatttatttc aaaaacgggt ctcgcgttgc gtattactga aaaagacttc
     8641 cgtatggaga tcgacatgcc aatcgacacg accctgtacg tccatcaaac gactttgcag
     8701 cttattgaag ccgcatgcgt catgacggga atcagacgcg gacagctcgt agttctgttg
     8761 ctcgcgcggg ccatcctgaa ccgcgggaag gcgctcaaat cgggctctgc gacgcgatat
     8821 caaacgcgcg accgcaggga gatgtggcgg accattcatg ttcgcatgtt agcggacgat
     8881 gccgattact tcagggacgc tcggtgtttt ttcaatgaat cggtttcgtt gattctggcg
     8941 caggcggcga gagaatacct ccgtgagata cttcaacgat cgagaagcgg cgagaaagtg
     9001 gataactatc cacttaaaaa ttacatcatc tgcagggagg aacgggacgg attgattttc
     9061 tggcacatct gctggggatt cccagaaaaa cctgaaaaat acttccgtct gtagctcgcg
     9121 acagacgcac ccggaaacga aaaaacgcca ttatacattc ggcaatcgag ttgttgcgat
     9181 acacggcgtc cggcaactac agcgcgcttc ggaaattacc gccgcacccc gcatcattcc
     9241 tcggccgcac cggcgtcttc ttcatcgtcg agcggaccaa gctcccgcag caccgcacga
     9301 attaccgcgg catcgaaacc ccggccctgc agataaaagg cgagccgcga ggcgcggttt
     9361 ttctttccgg cgcgctgccg aagtttcttt cgcgcgcatg cgagcgccgc ttcccgctcg
     9421 tcgtcttccg acgttgcgcg cgagatcgcg gttgcgataa tgtcccgcga aacccccttt
     9481 cgctgcagct cgcgcgtgac gaggttcctg cccacgccgc cgcggcgttt tcgcgcaccg
     9541 gcatactccg tcgcgaaggc gagatcgtcg atatagtgtc gctcgcacag atcgcgaacg
     9601 gtttccgcga tactgtcgcc ggaatagccc ttgcggcgaa ggtacgactc catctcatgc
     9661 gccgaatggg cgcgcaccgc cagatatgaa agcgccgccc gtcggcacat gtaccgatcg
     9721 ctctcgtccc tgagctggcg atacgccgcc gcgtcgatct cggtcccggc cgcgagccga
     9781 tactgcgcaa agaccgcggc ggaaaccatg agcgcctccc ccgtatcgac ggatatcatc
     9841 accccgtcgc ccttgaactt gattttttcc accagcggca tggagacgcc cctagagacc
     9901 gcgcaggaaa ccggcaagac cggggctgat ccgcttcgaa tggagcgaat cgcggtagac
     9961 gaatttcacg tgcaggcagg cgggcgacag gttgaattcc atgggctcgg ggagcagggc
    10021 gacgccgcgg cagaagaact ggatggagcc gtcataagcg catttgtaca gcggggcgct
    10081 ctcggatgcg acgccgtcga cgacgagccg caccgtgacg ggatcggtga cggcgacgac
    10141 gcgcgacccg cggcgtatct cgatccggtc cgtttcggcg tgaagccggc gcttcagctc
    10201 gctgaatcca tgggcgccgc agccggccat ggacagcacg agcgccagcg tcatcatcaa
    10261 caaccatgtc cgaaatatgc cctgcatagc gcacacacct caccggttgg atgcaccgca
    10321 tgaaaaaagg gtgccgataa aatcgacacc cttttgtttc caaaggcaag taaaaaactg
    10381 gcgtttcgtt tagcgcttgg agaactggaa gcgcttacgg gcctttttac ggccgtactt
    10441 ctttcgctcc acctggcgcg aatcccgcgt gagaaaaccg gcctcgcgga gcgccttctt
    10501 gttgcgctca tcatgctgga tcaggcaccg cgcgatcccc atgcgcaccg caccggcctg
    10561 gccggaccag ccgccgccga gcgttgtcgc ttcaatgtcg aatgcgctga cggcgctcag
    10621 gagctcgagc ggctgccgta cgatgagctc gagggtttta cggcgaaaaa actcggatac
    10681 gtcctgctca tttacgagaa tcttccccga accgggcttg agccacaccc gcgcgaccga
    10741 ggtctttctt ctaccggttg cataataccg ttctgctgcc atgcgatcct gctcctgtcg
    10801 tatattcgat tatttcttga tatcgtacgt ttcgggcttt tgcgcctgat gggggtgggc
    10861 ggtccccgcg tagaccttca ggttcttgaa catcttgcgg cccagccgat tcttgggaag
    10921 catccccttc acggcgtgct cgatgatgaa ttccggatta tccgccttgt acttcttgat
    10981 gttggtgaac ttcacgccgc cgggaaagtg cgaatggctg aaatactcct tgtcgtcgcc
    11041 cttgctgccg gtgagcacca ccttgtcggc gttgatgacg atgacgttgt cgccatcatc
    11101 catgtgctgg gtgtaggtgg tcttgtgttt tccgcgaagc aggtgcgcga cctccgtcgc
    11161 gagccggccg aggaccgccc catcggcgtc gacgaggagc cacttcttct cgatttgcga
    11221 tgccttgatt gaaaacgtgc gctgcgaact attcattggg tgtttcctga aaaccgataa
    11281 aaattgagcc ggtggcatca cgaacgaggc ctacggcgat gcagtgggca acgctaaatg
    11341 tgagcacctg atacgtcaat aaaatttttt catcaaaaat ccacctttcc gcttgacggt
    11401 accgataact atcggtatat tatgaccgaa cagtcggtca atataaaaac tatccccggt
    11461 ggggattttt aaaagaatct ttaatgaccg aatagtcggt caaacaggaa aagagatgtc
    11521 tccaaagatt gtggatgttg atgagaaaaa gaagcagata accgacgccg ccatacgggt
    11581 cctcgccagg aagggcctca aggaaacgaa gatggaggac gtcgccgaag cagcgggtat
    11641 cgggaaaggc accctgtatg aatacttcaa gtcaaaagat gaactcttct tggtcgccta
    11701 tcgtcatatt atggaagaat tcgaatgcca aaccgatttg aagctgccgg aaaatgtaac
    11761 cacgatagac accctgaaag cgatcatttc gatgatgacc tcgatgcaca cttcggactt
    11821 cccgctggat cacttccagg tgctcaccga gttctggctc gaggaggtca aatcggcaaa
    11881 ccaggggaaa aagcggcaat tcgacatgct cgccacttac cgcgagtata tcgacctcgt
    11941 gaaagcggtg ttcgaacagg ggattcaaaa aggggaaatc cgcgacatca accccgaaca
    12001 cgccgccacc attctcatga tgatcttcga cggcctcatg ctcctctgga tgctcgacaa
    12061 gaactccttc gacttcaagg gcaccgtgga cacgttcggt ggcatattca taaaaggaat
    12121 tcaaaagcag gggtaggata tggcgatcaa aacggacgcg ccatcatgcg gcgcaacctc
    12181 accccctacc cctctcccat aggagagggg agtggaaaaa gctacttccc acgaagcccc
    12241 ctcctatggg agagggcgcg ccggaggcgg ggtgaggttg aacgagcgta tgtatgaata
    12301 aatcaaggat aaggagaaaa cccatgaagt cattaatgat cattctcgcg ctgatcacgg
    12361 cgctcgcgtc aacggcactg ccgtcatacg ccctcacggg caaggagatc atcagaaaga
    12421 ccgaggacgc catgcgcggc acctccgtcg tgggcaccta cctcatcacc gtgaagacga
    12481 agctgtggac gcggacgatg aagttgaagg catacgacca gcgcgccggg aagaagtcct
    12541 tcaacgagat ctacgaaccg aaaaaggacg cgggcaaccg gttcctcatg atcaacaaga
    12601 acatgtggca ctatgtcccc aagctccagc agaccatcaa gatttcgccg tcgatgatgc
    12661 tccagtcgtg gatggggtcg gacttcacca acgacgacat cgtgaaggaa tccagcatcg
    12721 aggaggacta cacccacacg gtcaccggga ccgcgacggt ggacgggcac ccctgccatg
    12781 tcatcctgct cacgccgaag cccgacgccg ccgtcgtatg gggcaagata atctactccg
    12841 tgcgacaaag cgactacctc cccgtgcgcg aggaattcta caacgagcat aacgtcctca
    12901 agcgggtgat gaccttcggc cagttcaaga agatggacga tcgcgtgatc cccaccctct
    12961 ggaagatgca aaccgtgagc aagccggagg cgcacacgat cctcgagatc gcgagcatcc
    13021 ggtttaacac cgcgatcccg tcacgcacct tcaccatgca gaacctgacg aggaagtagc
    13081 catgaaccga gaagcgatcg cacagctcgc ctggcgaaac gtgtggcgca acaagcgccg
    13141 gacgatcctg acgctcgcca ccatcgtcgt cggctgcggc atgatcatcc tcaacaacgc
    13201 cctggccaag ggcgggcatg acgggatgat caacgacgcg gcgatcacga gcgccgggca
    13261 catccaggtg caccgcgccg gctactgggc gagccgcacc atcgactacg cgttccaggt
    13321 gccggacggc ctcaccgccg cgctcgggcg agaccgtcgg atcgccgcgc acaccgagcg
    13381 catccatgcc gccgggctcc tcgccttcgg cgcgaacacc tccggcgggc agatccaggg
    13441 cgtggatccc gcgcgcgagc cggcggtgac caacctgcac acgtgggtcc ttcccggcgg
    13501 gcgctatctc accgcggagg attcgaccca cgcgatcgtc ggggagacgc tcgcgaagaa
    13561 cctcggcgcg aaggtcggcg acgtcgtctc gatcatctcg caggggctcg acgggtccat
    13621 cgccgccgag aagctcacgg
//
LOCUS       DAORHF010000085        13549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3606_length_13549_cov_5.131021, whole genome shotgun sequence.
ACCESSION   DAORHF010000085 DAORHF010000000
VERSION     DAORHF010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_13549_cov_5.131021"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PKJ16_08795"
     CDS             <1..432
                     /locus_tag="PKJ16_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV47126.1"
                     /translation="PVRLRKVVLFLVEDAVYAFDADEVKEVVLAGEIYPIPFVPAYVS
                     GLINRHGEPHTVIDLKAVLGGGRLDASRVLIMNNGSDQISFLITDIDKIAEIPEPDVV
                     SLPATDGGALFKGSFRCEGREVFIINPQEIYDRLGADIKSV"
     gene            446..967
                     /locus_tag="PKJ16_08800"
     CDS             446..967
                     /locus_tag="PKJ16_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47127.1"
                     /translation="MIPCAGADFLINREEILHSRYEGEHQRAAGRAAPVRVMEFEGRA
                     IPVINLDGHIEEHFGMVPHRGPGILLFIRGSGDHPPFPLMRRKSDGTDVETSRVALRT
                     SAMVSMLSVGIDELKPFPPRLRESLAAGGLLAVRFPRDGGRAQYLLDMRSTAILCIAA
                     YHNRQVADGHPDS"
     gene            948..1952
                     /locus_tag="PKJ16_08805"
     CDS             948..1952
                     /locus_tag="PKJ16_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HNV47128.1"
                     /translation="MDILIVDDSALLRNILKQVLAKEDGISIAGEASNGVQAVELAAA
                     LSPDLVIMDIDMPLLDGIAATAKIMSKSPVPILILSSVASPENVFKALRNGALEVMKK
                     PSIDQFNNPEFFAGFIEKLKTLSHVSVEGGAGASVERHRADKVSGAASLFVMGASTGG
                     PVTLAAILSRLSSGFPAGIALVQHLEAGFENGFVSWLAAESGMRVRLAAEGDYPAAGE
                     VLLAPPGRHLVFASGRLAFEDSAPVVNQKPSVNRLFVTAAECFRGRVAGVLLTGMGSD
                     GADGCVEIIKRGGTTIVQDEATATVFGMPRAAIEKGGAGIVLPKESIADYMLKLAMAR
                     "
     gene            2039..3373
                     /locus_tag="PKJ16_08810"
     CDS             2039..3373
                     /locus_tag="PKJ16_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HNV47129.1"
                     /translation="MRNLENVIVALSAARGMRPAGYLAALSRDGREVERFLDRITNNE
                     TYFFREERHFTALKDEVFPRLATHGGPIVIWSATCSTGEEAWSLCALARECLGDAFRV
                     VGTDINHDSLRVAETGSYSKNSFRADGAAYRGLIEGNTGRADGRWVVSDELRRRVEWR
                     RCNLYADPAERMPEGAHVVFFRNTLIYMRQEIKQAVVEKIARRMAPGGHLFLASSEMP
                     LIVNPGLALAEYRNTYYFVRAVAAERPRASQDQSVVAPIVRDAAEERPAARVMHAPEA
                     AVLSAAESLHRDPFFRLSERDPAHLAAGLMHYALFFVNRMNIAAAAEAVRLAELFTGG
                     AVVLHLKGFVAMAGGDAAGAAGFFRGALAADGGFWPSRFYLASLLRVSDGVRAAEEFR
                     RCRRDIAAAAGSGDARFRFLLEGFSEKYFMEICDNWIATGGKPRRGVAKLWR"
     gene            3364..5505
                     /locus_tag="PKJ16_08815"
     CDS             3364..5505
                     /locus_tag="PKJ16_08815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47130.1"
                     /translation="MALKRESFVGQYLDELEENVVNLEKSIAALRRDSDSEEETNRAV
                     RILHTIKGSSRMLKFATIEKLAHGLESVIKGVKEKRYPVSQEIVQLVLAGAGRIRDGA
                     ESIRSTGDFHADIGPLLANCERAYAGEPFDPGDAGRSARVSSAPVERRSEPRRLPTDE
                     SATIRVRTGTIDEIIRMLNNLIVNQFQFKKHHGAIAAIEELLEGRGGFDDDGELLKSF
                     RMMRKSFKEQIEIIERDTFALQERIIGMRMFPLEMIMGSLPGMVEELAISLGKLVNLN
                     LSGTDAALDRLILETIHDPIIHIVRNAVDHGIETPDEREGAGKPRTAELTITCRPESG
                     GISISIADDGRGIDTEAVRRRACALYPARRDEIESMKDEELNALIFSPGFSTAAAVTA
                     LSGRGAGLDIVKHNLEKVKGRIFIESRPGRGTVFTLTLPLTLSTIDGYFVRSAGMKYF
                     VPANYVNEVLIVERRDVLKVLNRDAIRVRDAIIPLFSLPVLLNERPAEDRESGFVFIA
                     ESLGSYIGVAVDSVLEYVTLIYKPLPESVRGIEYIQGIVFDERYDIVSILHMPALIER
                     AKRLSDIDLKSRYAGGGRRDRRVLVVDDSLNSREIQRSILESAGCDVTTVEDGIGALE
                     SLRARDFDIVITDINMPRMDGFTLLENLKRDPAVRSIPVIVVSNYDDVETSRRALELG
                     ASSYIVKSDFDRNNLVETVDALLRARGEHDG"
     gene            5498..7354
                     /locus_tag="PKJ16_08820"
     CDS             5498..7354
                     /locus_tag="PKJ16_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47131.1"
                     /translation="MDNLKKILVIDDSDLVAGMLSSLLEKSGYATSRAVNGLEGVIAV
                     YREAPDLVIMDVEMPVLQGYQASRLLKARRGVCDIPIIMHTSLSGDRDEFWALSSGAD
                     AFITKDFDNLMPLLDSIGELIGARAVNRSIIAEDAAKVDRDCVMEMLSTTLDGHLFQS
                     VILNRLSETAACIGSLSDTGVSVLSLIDRVCSGDIAVLLLKSEGEVAAFIRPGPGMDE
                     AAALDFYRICVDDFYGAFPDAPRTDVAPVFAEQPRPSAAFDAPDERVAIRSYAHWVIP
                     GRCAPVGTLHVGSRVNNYFTAPISANVNVFADGAGSIIENAMLLKNITTMERNIRNVF
                     SKFVPAEIIDDLAGKSRSQGLRAGEKRNVAILFTDIRSFTEITEKNRPEDVVALLNRY
                     FSTMAEAVIRHGGTIDKYIGDAILAVFGAPRAYSNNAERAVSAAFDMLRSLQNIDTAG
                     LIMPDGRLDMGIGIHEGDVIVGNIGSKERFDYTVIGDAVNLASRLEGLTKMYRSHVIV
                     SESVRAALKDAMTFRELDRVRVKGKGIPTTIFKLENVTRMRFSEEQLRDYAKAVSMYK
                     IKNWDTAIEYFNKVLEHNPDDYVSAMFVSRCEEYRRNPPPDDWDGAISFDGK"
     gene            7438..8139
                     /locus_tag="PKJ16_08825"
     CDS             7438..8139
                     /locus_tag="PKJ16_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47132.1"
                     /translation="MSCRRTEGSCVAARAGTLRAMCLAALMALTISCSTMSAAWYSQP
                     EPRYRSRAHGGMAALVEAGGVRHVILSLSNRYKVYMGPPLVPVRDMEPPILNEQTFEL
                     RWQIRPPGPVAPGTSMGWRLVREALAIRSNDGTVITPTHISIEGLDHGSGETLYVHYA
                     YEEVPAVVPVARPVEVTLRFYNLSYGRMPKRYSFEPALHAGDERIAAPRVQFVLKRDY
                     EYKPCVVPLYFMSVR"
     gene            complement(8169..9341)
                     /locus_tag="PKJ16_08830"
     CDS             complement(8169..9341)
                     /locus_tag="PKJ16_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV47133.1"
                     /translation="METKKVKINLSYLRPNSTFIYPLFSEEGKKILDERIVLTAELIK
                     KITARFGNFVYYAEDGKRAVIQEGKLRAALATSHDVLDEIARTSKLSAEKYHEAERLV
                     DDIIHNLSVEHANVINLIKDLKSFDDYLYNHSVNVGLLTAVFAKKIGGFSDHQYQSIT
                     LGAYLHDIGQRKIDKQLLHKQGQLDVLELTKLKRHPQLGYEILKDLEDSNETVLQTVL
                     FHHEKYNHKGYYQLPYENLPIFPKLASICDIYDALTSPRPFREEPFTSCMALKAIVNS
                     ISSHFDFTLVSRFVNLMGPILNNDRPIFAVNDICELNTGELALVKGITPGDILKPQVV
                     VFCRFVKEGDRLGVKHYNEPTAADLATENKRVVKKILNNRKQVDAIQKMAKERGLL"
     gene            9465..11042
                     /locus_tag="PKJ16_08835"
     CDS             9465..11042
                     /locus_tag="PKJ16_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HNV47134.1"
                     /translation="MRIVIPAIILSLAALASCRDMSAVRPPDTLAIHMGAEPSHLNPI
                     TSTEAVASSINNYIYENLVDRDYDTLEIVPQLAERWTISPDRLRYVFHLKRGVVWSDG
                     VEFTADDVVYSYERIKDPKVACAPLKVYYIDVTSCRALDRYTVEFVYSKRYFRALEIC
                     GGMPLVPKHVFNDGTDFNTHTKNRSPVGTGPYRFERWDTGKRIVLIANERYRGKKPAV
                     RRVVYTIVAEINVALQMLKKGELDLMGVRPIQWVRQTNSESFLSRYRKLKYFMPYFSY
                     IGWNSRRAWFSDKRVRRALTHLVNREAILEKLEFGQGMVVSGDSYVFSKNYNQNIKPW
                     PYDPAAARELLRQAGWVDSDRDGILDKDGKKFSFTFTMSSGSKFAERLSTILKEDFAK
                     AGIQMSINRYEWAVFVKKLHERDFDAVTLAWSLDWESDPYQLWHSSQVKDGSNYCGFV
                     NREADGIIERVRAEFDPVARMRLLHRFHEILHEEQPYTFLYCTPALVVVSKRFNNVVV
                     HTKGLRYDEWEPAGGAQ"
     gene            11039..12040
                     /locus_tag="PKJ16_08840"
     CDS             11039..12040
                     /locus_tag="PKJ16_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47135.1"
                     /translation="MKKYLVKRLLLLIPTFIGITLITYLMVRLAPGDYTTLRAGVQGE
                     LKQGAIGKEIIEQEKKLFGLDKPIPVGYAEWLWKFVRLDFGISRKDGRPVSARIAEAL
                     PITLRLNLIAMIVVYIISIPMGIFSAVRKDSTYDRVSSLALFVLYSLPSFWVGLLLLL
                     VFSGGDYLNLFPLGGEISDWARDFGFFGRLGNRIWHLVLPVVTLTYGSFAFLSRYARA
                     NMLEVINQQYITTARAKGLSDNSVVFVHAFRNSLVPLVTLMATLLLGLLGGSVIVEQI
                     FSIPGMGLLAFEAILSRDIPVIMAIAAISALLTLLGILLADIMYGLVDPRIRLEAKL"
     gene            12037..13023
                     /locus_tag="PKJ16_08845"
     CDS             12037..13023
                     /locus_tag="PKJ16_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47136.1"
                     /translation="MSAQGYWSNVWREFRRNRFALAGLSIVAILFVIALLAPLIANDR
                     PYVFVDGGRAYFPLFKDYPALKDVNFRTTRGSFSVMPPIPYSYSAYDLDAIVLPPSAR
                     HVLGTDEQGRDLAARMVYGTRVSILVGFIAVFIYVSIGIVVGAVAGYYGGRTDMLISR
                     FIEIVMCFPTFFLILTILALVGPSLTSVMVVIGLTGWTGIARIVRGEFLKLREADYVT
                     ASRALGAGDARIIMRHVLPNALAPVLVSATFGIASTILIESSLSFLGFGVQPPTPSWG
                     DILSQSRDFMDFAWWLTLIPGFAIFITITSYNLVGEGFQDAIDPKALKRGRD"
     gene            13033..>13549
                     /locus_tag="PKJ16_08850"
     CDS             13033..>13549
                     /locus_tag="PKJ16_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47137.1"
                     /translation="MDQKLIEEVINRSIKKLNDEYELINTTFLNVLHANIDQLIENTE
                     AWFGPAAPKEFKDVLNSLMTLAYLYYEERREYSKYIVEEKYENVDLAELMDDIIEKVR
                     FLFPARAVTLVAAESDCSIRASRSLVRESFFNILLSLFQFMDERSSCGIRLFTEGQNV
                     KIALAFDNLAEE"
BASE COUNT         2453 a         4105 c         4402 g         2589 t
ORIGIN      
        1 ccggtccggc tcaggaaggt ggtgctcttc ctggtcgagg atgccgtgta tgccttcgac
       61 gccgacgagg tgaaggaggt cgtcctggcg ggggagattt atcccatccc cttcgttccc
      121 gcctacgtgt cgggactcat caaccggcac ggggagccgc ataccgtcat cgacctgaag
      181 gcggtgttgg gcggcgggcg cctggacgcg tcccgcgtgc tcatcatgaa taacgggagc
      241 gatcagatct cgttcctcat caccgatatc gataaaatcg ccgagatccc ggaacccgac
      301 gtcgtttcgc tcccggcgac cgatggcggc gccctcttca agggttcgtt ccggtgcgag
      361 ggccgggagg tcttcatcat caatccgcag gagatatatg acaggcttgg cgccgacatc
      421 aagagcgtct gaggcggatt tcgccgtgat cccgtgcgcg ggggcggatt ttttaattaa
      481 ccgggaggag atccttcaca gccgatacga gggggagcat cagcgggccg cgggcagggc
      541 tgctccggtg cgcgtcatgg aatttgaggg gcgcgcgata cccgtcatca acctcgacgg
      601 acacatcgaa gagcatttcg ggatggtccc gcaccggggg cccggcatac tgctgttcat
      661 tcgcggctcg ggcgatcatc ccccgtttcc gctcatgcga cgtaaatcag acggcacaga
      721 tgtggaaacc tcgcgcgtgg cgctgcgcac ctccgccatg gtgtcgatgc tgagcgtcgg
      781 catcgacgag ctcaagccgt tccccccgcg cctgcgggag agccttgcgg cagggggcct
      841 cctcgcggtg cgttttccgc gtgacggggg gagggcgcag tatctgctgg acatgcggag
      901 caccgcgatc ctgtgcatcg cggcatatca caacaggcag gtcgcggatg gacatcctga
      961 tagttgacga ctcggcgttg ctgagaaaca tcctgaagca ggtgctcgcc aaggaggacg
     1021 ggatttcgat cgccggcgag gcgtccaatg gcgttcaggc ggtcgagctc gccgcggccc
     1081 tctcgcccga cctggtgatc atggacatcg acatgcccct cctggacggg atcgccgcga
     1141 cggcgaagat catgtcgaag tccccggtcc ccatactcat cctgtcgagc gtggccagcc
     1201 ccgagaacgt gttcaaggcg cttcgcaacg gcgcgctcga ggtgatgaaa aagccctcca
     1261 tcgatcaatt caacaatccc gagttcttcg ccggattcat cgaaaaactg aaaaccctga
     1321 gtcacgtctc ggtggagggc ggcgcggggg cgtcggttga acgacaccgc gcggataaag
     1381 tgtccggagc ggcctccctg ttcgtcatgg gcgcgtcgac cgggggcccg gtcacgctcg
     1441 cggcgatact ctctaggctg tcatccggct tcccggcggg tatcgcgctt gtccagcacc
     1501 tggaggcggg attcgagaac ggattcgtga gttggcttgc cgccgaatcc ggaatgcgcg
     1561 tccgactggc cgcagagggc gattaccccg cggcaggaga ggtgctgctg gccccgccgg
     1621 gccgacatct cgtgtttgca tctggccggc tcgcctttga ggactcggcg ccggtggtga
     1681 atcaaaagcc cagcgtgaac cgcttgttcg tgaccgccgc cgagtgcttt cgagggcggg
     1741 tcgccggcgt gctgctcacg ggcatgggct ccgacggcgc cgacggctgc gtcgagatca
     1801 tcaagcgcgg ggggacgacg atcgtccagg acgaagcgac ggcgaccgtg ttcgggatgc
     1861 cgcgcgcggc gatcgagaag ggcggcgcgg ggatcgtgct tcctaaggaa agcatcgccg
     1921 actatatgct gaagctggcg atggcgcgat gacggcgcgt gcagcggcag atgtcccgat
     1981 cggggatttc gtccgcctgg tcgaacgcac ggccggtata accgtacccg atgccgatat
     2041 gagaaatctg gagaacgtca tcgtcgcgct ttctgccgct cgcgggatgc ggcccgcggg
     2101 atatctggcc gccctctcgc gcgatgggcg tgaggtggaa cggttcctgg accggataac
     2161 gaacaacgaa acatactttt tccgcgagga gcggcatttc acggcgctca aggacgaggt
     2221 cttcccgcgc ctcgcgacgc acggcggccc gatcgtcatc tggtccgcga cctgttcgac
     2281 gggggaggag gcgtggtcgc tctgtgcgct cgcgcgtgaa tgcctcggcg acgcgttcag
     2341 ggtggtcggc accgatatca accatgattc gctgcgcgtc gcggagaccg gatcgtactc
     2401 gaaaaactcg ttcagggccg acggcgccgc ataccgcggg ctcatcgaag ggaacaccgg
     2461 tcgcgccgac gggcgctggg tcgtttccga cgagctcagg cggcgcgtcg agtggcgccg
     2521 ctgcaacctc tacgccgatc ccgcggagcg catgcccgag ggcgcgcatg tggtgttctt
     2581 ccgaaacacg ctcatttaca tgcggcagga gataaagcag gcggtcgtcg agaagatcgc
     2641 gcgccgcatg gcgcccgggg gccatctgtt cctggcgtcg tcggagatgc ccttgatcgt
     2701 caatcccggg cttgcccttg cggaataccg caatacctat tacttcgttc gggccgtcgc
     2761 agcggaacgc ccgcgagcgt cgcaggacca gtcagtcgtc gctcccatcg ttcgggacgc
     2821 cgctgaggaa cggccggcgg ctcgggtgat gcatgcgccg gaggcggcgg tgctatctgc
     2881 ggcggagtcc ctgcatcgtg acccgttctt tcgcctctcg gagcgcgatc cggcccatct
     2941 ggccgcggga ttgatgcact atgcgctttt cttcgtcaac aggatgaaca tcgcggccgc
     3001 cgcggaagcg gtgcgcctgg cggagctgtt caccggcgga gcggttgtgc tgcacctgaa
     3061 gggcttcgtc gctatggcag ggggcgatgc ggccggggcg gccgggttct tccgcggggc
     3121 gctcgcggcc gacggcggct tctggccgtc acggttctat ctggcctcgc tgctcagggt
     3181 gagcgacggc gtcagggccg cggaggagtt tagaaggtgc cggcgggaca tcgcggcggc
     3241 ggcgggctcc ggagacgcgc gattccggtt cctgctggag gggtttagcg agaaatactt
     3301 catggagata tgcgacaact ggatcgcaac gggcggaaaa ccgcgcaggg gggtcgcgaa
     3361 gctatggcgc tgaagaggga gtcgttcgtg gggcagtatc tcgacgagct cgaggagaac
     3421 gtcgtgaacc tggagaaatc gatcgccgcc cttcggcgcg attccgactc cgaggaggag
     3481 acgaaccggg cggtgcgcat cctgcacacc atcaagggtt cctcccgcat gctcaagttc
     3541 gcgacgatcg agaagctcgc ccacgggctg gaaagcgtca tcaagggcgt caaggagaag
     3601 cgctatccgg tgagccagga gatcgtacag ctcgtgctcg cgggggccgg gagaataagg
     3661 gacggggccg agagcattcg ctctacgggc gattttcacg cggacatcgg cccgctgctg
     3721 gccaactgcg agcgcgccta tgcaggcgag ccgttcgatc cgggcgacgc gggcagatcc
     3781 gcgcgcgtct cgtccgctcc cgtcgagcgg agatcggaac cgcgacggct gccgaccgac
     3841 gagagcgcga ccatccgggt gcggaccggg accatcgacg agatcatccg tatgttgaac
     3901 aatctcatcg tcaaccagtt tcagttcaag aagcatcatg gcgccatcgc cgcgatcgag
     3961 gagctgctcg aggggcgcgg cggattcgat gatgacgggg agctcctcaa atcattccgc
     4021 atgatgcgaa aatcgttcaa ggagcaaatc gaaatcatcg agcgcgatac gttcgcattg
     4081 caggagcgga tcatcggcat gcggatgttc ccgcttgaaa tgatcatggg gagcctgccc
     4141 ggcatggtcg aggagctcgc catctccctc ggcaagctgg tgaacctgaa cctgagcggt
     4201 acggacgcgg cgctcgatcg cctcattctc gaaacgatcc acgatccgat catccacatc
     4261 gtgcgcaatg cggtcgatca cgggatcgag acgcccgatg agcgggaggg cgcggggaag
     4321 ccccggacgg ccgagctgac catcacctgc aggccggaaa gcggggggat cagcatctcc
     4381 atcgccgatg acggacgggg tatcgacact gaagcggtgc ggcggcgggc ctgcgcgctc
     4441 tatccggcca gacgggacga gattgaatcc atgaaagacg aggagctgaa cgccctcatt
     4501 ttttcgccgg gattctcgac cgccgcggcg gtgaccgcgt tgtccgggag gggcgccggg
     4561 ctcgatatcg tgaaacacaa cctggagaag gtgaaggggc gcatcttcat tgagagccgg
     4621 ccgggaaggg gcacggtgtt caccctgacc cttcccctga ccctgtcgac catcgacgga
     4681 tacttcgtca ggtccgcggg gatgaaatac ttcgtaccgg caaactacgt caacgaggtg
     4741 ctgattgtcg aacgccgcga cgtcctgaag gtcctcaatc gggacgcgat ccgggtgcgc
     4801 gacgcgatca ttcccctgtt ctcgctcccg gtcctgctca acgaacggcc ggccgaggat
     4861 cgggagagcg gcttcgtgtt catcgccgaa tcgctcggca gctacatcgg cgtcgcggtg
     4921 gattccgtcc tggagtatgt gacgctgatc tacaaaccgc tgccggaaag cgtgcgggga
     4981 atcgagtaca tacaggggat cgtgttcgat gaacgctacg atatcgtgag catcctgcac
     5041 atgccggcgc tcattgaacg ggcgaaacgg ctctccgaca tagatctgaa atcgcgttac
     5101 gcgggcgggg ggcgcaggga ccggcgggtc ttggtcgtgg atgattcgct caactcgcgc
     5161 gagatacagc ggtcgattct cgagtccgcc gggtgcgacg tgacgacggt cgaggacggg
     5221 atcggcgcac tcgagtcgct ccgcgcgcgc gacttcgaca tcgtcatcac cgacatcaac
     5281 atgccccgca tggacggctt tacgttgctc gagaacctga agcgggaccc ggccgtgcgg
     5341 tcgatcccgg tgatcgtggt gagcaattac gacgacgtcg aaacctcgcg ccgcgcgctg
     5401 gagctcggcg cctcgtcgta tatcgtcaag tcggatttcg accgtaataa tctggtcgag
     5461 accgtcgacg cactgctcag ggcgcgcgga gaacacgatg gataacttga aaaaaattct
     5521 tgtcattgac gactctgacc tcgtcgcggg gatgctctcc tcgcttctcg agaagagcgg
     5581 atacgcgacg tcgcgtgcgg tgaacgggct cgagggcgtc atcgccgtgt accgggaggc
     5641 gcccgatctg gtcatcatgg acgtcgagat gccggtgctg caggggtatc aggcgagcag
     5701 gctgttaaag gcgcggcgcg gggtgtgcga tattcccatc atcatgcaca cctccctcag
     5761 cggagaccgg gacgaattct gggcgctttc ctcgggggcg gacgccttca tcacgaagga
     5821 cttcgacaat ctcatgccgc tgctggactc tatcggcgaa ctcattggcg ctcgcgcggt
     5881 gaaccgatcg atcatcgccg aggatgccgc gaaggtggac cgggactgcg tgatggaaat
     5941 gctgagcacg acgctcgacg gccacctgtt tcagtcggtc atcctcaatc ggctctcgga
     6001 aacggcggct tgcatcggct cgctgtccga cacgggcgtg tccgtgctct cgttgatcga
     6061 ccgggtgtgt tccggggaca tcgcggtcct gctgttgaag agcgaaggcg aagtggccgc
     6121 ctttatacgg cccggaccgg ggatggacga ggccgccgcg ctcgacttct acagaatatg
     6181 cgtggacgac ttctacggcg cctttcccga cgccccccga accgacgttg cgcccgtgtt
     6241 tgccgagcag ccccggccga gcgcggcgtt tgatgcgccc gacgagcgcg tcgccatacg
     6301 ctcgtatgcg cactgggtca ttcccggccg gtgcgccccg gttgggacgc tgcacgtggg
     6361 cagcagggtg aacaactact tcaccgcgcc catcagcgcc aacgtgaatg tcttcgcgga
     6421 cggcgcgggg tcgatcattg aaaacgccat gctcctcaag aacatcacca ccatggagcg
     6481 gaatatccgg aacgtcttct cgaaattcgt tcccgccgag atcattgacg atcttgccgg
     6541 caagagcagg agccagggtt tgcgcgccgg ggaaaagcgg aacgtggcca tactcttcac
     6601 cgatatccgg tcgttcaccg agattaccga gaagaacagg ccggaggacg tcgtcgcatt
     6661 gctgaaccga tatttcagca ccatggccga ggcggtcatc cggcacggcg ggaccatcga
     6721 caagtatatc ggagacgcca tactcgcggt gttcggggcc ccgcgcgcgt acagcaataa
     6781 cgccgagcgc gccgtgagcg ccgcattcga catgctgcgg tcgctgcaaa acatcgacac
     6841 cgccgggctc ataatgcccg acggacggct cgacatgggc attgggatcc acgagggcga
     6901 tgtgatcgtg gggaacatcg gatcgaagga gcgcttcgat tatacggtca tcggcgatgc
     6961 ggtcaatctc gcctcccggc tcgaggggct tacgaagatg taccggagtc atgtcatcgt
     7021 ctccgagagc gtgcgagcgg ccctcaagga cgccatgacc ttccgcgagc tcgaccgggt
     7081 gcgggtgaag ggcaagggga tccccacgac gatattcaag ctcgagaacg tcacgcggat
     7141 gcgattctcg gaggagcagc tgcgcgatta tgccaaggcg gtcagcatgt acaagatcaa
     7201 gaattgggat accgcgatcg agtacttcaa caaggtgctg gaacacaacc ccgacgatta
     7261 cgtttcggcc atgttcgtct cgcggtgcga ggaataccgc cggaatcccc cgcccgacga
     7321 ctgggacggc gccatctcgt ttgacgggaa atgatccgct tggcgcgcga acggcggaat
     7381 cggcggtcgc gatgcgcctg gagaacgcga ccgacgatgc caggaggcag tgaatctatg
     7441 agctgcagaa gaaccgaagg atcgtgcgtc gctgcgcgcg cgggcaccct gcgggcgatg
     7501 tgcctcgcgg cgctcatggc gctaacgata tcgtgcagca ccatgtcggc ggcctggtac
     7561 tcgcagccgg aaccgcgcta tcggtctcgc gcgcacggcg gcatggccgc gctggtggag
     7621 gccgggggcg tgcggcacgt gattttgtca ttgtccaatc gctacaaggt ttacatgggg
     7681 ccgccgctcg tacccgtccg ggacatggag ccgcccatac tgaacgaaca gaccttcgaa
     7741 ctcaggtggc agatccgccc gccaggcccg gtcgcgccgg ggacgtcaat ggggtggcgg
     7801 ctcgtcaggg aggcgctcgc catccgctcc aacgacggaa cggtgattac gccaacgcac
     7861 atttccatcg aggggctgga tcatggcagc ggcgagacgc tgtatgtcca ttacgcctat
     7921 gaggaggttc cggccgtcgt ccctgtcgcc cggccggtgg aggtgaccct gcgattctat
     7981 aatctctcat atgggcgaat gccgaagagg tattcgttcg agcccgctct acacgcgggg
     8041 gatgagcgca tcgcagctcc ccgcgtgcaa tttgtcctga agcgcgatta cgaatacaaa
     8101 ccctgcgtcg taccgctgta tttcatgtca gtgcgctagg ggagagcgag cggagcacgg
     8161 caattccgct acagcaaccc gcgctccttc gccatcttct gaatggcgtc gacctgcttg
     8221 cgattgttga ggatcttctt cacgacgcgc ttgttttcgg tcgcgaggtc ggccgcggtg
     8281 ggctcgttgt aatgcttcac cccgagccgg tcgccctcct tgacgaaccg gcagaacacc
     8341 accacctgcg gcttcaggat gtcgcccggc gtgatgccct tcacaagggc gagctcgccg
     8401 gtgttgagct cgcagatatc gttcaccgcg aagatgggcc ggtcattgtt caggatgggg
     8461 cccatgaggt tcacgaaacg gctcaccagc gtaaaatcga aatgcgagct gatggagttc
     8521 acgatggcct tgagcgccat gcaggacgtg aagggctcct cgcggaacgg ccgcggcgag
     8581 gtgagcgcgt cgtagatgtc gcagatggag gcgagcttgg gaaagatggg caggttctcg
     8641 taggggagct gatagtagcc cttgtgattg tacttctcgt ggtggaacag caccgtctgc
     8701 agcaccgtct cgttggagtc ctcgaggtcc ttgaggatct cgtatcccag ctgggggtgg
     8761 cgcttgagct tggtgagctc cagcacgtcg agttggccct gcttgtgcag gagctgcttg
     8821 tcgatcttcc gctggccgat gtcgtggaga taggccccca gcgtgatgct ctggtactga
     8881 tggtccgaga acccgccgat cttcttggcg aacaccgccg tgagcagccc cacgttcacc
     8941 gagtgattat acaggtagtc gtcgaaactc tttaagtcct tgataaggtt gatgacgttc
     9001 gcgtgctcaa ccgagaggtt gtggatgatg tcgtccacca ggcgctcggc ctcgtggtac
     9061 ttctcggcgg agagcttgct cgtgcgggcg atctcgtcga gcacgtcgtg cgaggtggcc
     9121 agcgccgccc ggagctttcc ctcctggatg accgcgcgct tgccgtcttc ggcgtaatag
     9181 acgaagttcc caaagcgcgc ggtgatcttt ttaatgagct cggcggtgag gacgatgcgc
     9241 tcgtcgagga tctttttccc ctcctcggag aacagggggt aaatgaacgt cgagttgggc
     9301 cgcaggtagc ttaaatttat tttgaccttc ttggtttcca ttcccttcca agctcgaact
     9361 gtgtgtttct gtcaagagta gaatggcgcg cgcgggcgat attgacaagt aaaaaatcag
     9421 aatacttctt gacggaaacc ctcggccccc gcagaaacta gcccatgcgg atcgtaatcc
     9481 cggccatcat cctttcactt gcggcgctcg cctcgtgccg cgacatgtcg gcggtgcgtc
     9541 cgcccgacac gctcgcgatt catatgggcg cagagcccag ccatctgaac cccatcacct
     9601 ccaccgaggc ggtcgcgagc agcattaaca actacattta cgagaacctc gtggaccgcg
     9661 actacgacac gctggagatc gttccgcagc ttgcggagcg ctggaccatc tcgccggacc
     9721 gcctgcgcta tgtcttccat ctcaagcggg gggtggtgtg gagcgacggc gtcgagttca
     9781 cggcggacga tgtcgtctac tcctacgagc gcatcaagga cccgaaggtg gcctgcgcgc
     9841 cgctcaaggt gtactatatc gacgtgacca gctgccgcgc cctcgaccgc tacacggtag
     9901 agttcgtgta ttccaagcgc tatttccgcg cgctggagat ctgcggcggc atgccgctgg
     9961 tgcccaagca cgtgttcaat gacggaacgg acttcaatac gcacacgaaa aaccgatcgc
    10021 ccgtaggcac ggggccgtac cgcttcgagc gctgggacac cggcaagcgc atcgtgctca
    10081 ttgccaacga gcgctaccgg gggaagaagc ccgcggtgcg ccgcgtggtg tacaccatcg
    10141 tcgcagagat caacgtggcg ctgcagatgc ttaaaaaagg cgagctggac ctcatgggcg
    10201 tgcgccccat ccagtgggtg cggcaaacga actcggagtc gttcctttcg cgttaccgga
    10261 agctcaagta cttcatgccg tatttcagct atatcggctg gaacagccgc cgcgcgtggt
    10321 tttcggacaa gcgcgtccgg cgggccctca cgcacctggt gaatcgcgag gccatcctcg
    10381 agaagctgga gttcggccag ggaatggtcg tctcgggcga ttcgtatgtg ttcagtaaaa
    10441 attataatca gaacatcaag ccgtggccgt acgatcccgc cgccgcgcgc gagctcctcc
    10501 ggcaggccgg atgggtcgac tcggaccgcg acggcatcct ggacaaggac ggcaagaagt
    10561 tctcgttcac cttcaccatg tcctccggaa gcaagttcgc ggagcggctc tccaccatcc
    10621 tcaaggagga tttcgcgaaa gcgggcattc agatgagcat caaccgctac gagtgggcgg
    10681 tgtttgtcaa aaagctccac gagcgcgatt tcgacgccgt gacgctggcc tggtcgctcg
    10741 actgggagag cgatccctac cagctctggc attcctcgca ggtgaaggac ggatcgaact
    10801 actgcggctt cgtgaaccgc gaggccgacg gcatcatcga gcgcgtgcgc gcggagttcg
    10861 atcccgtcgc gcggatgcgc ctgctccacc gcttccacga aattctgcac gaggagcagc
    10921 cgtacacctt cctgtactgc acccccgcgc tggtggtggt gagcaagcgc ttcaacaatg
    10981 tggtcgtgca caccaagggc ctgcgctacg atgaatggga gccggcgggg ggcgcgcaat
    11041 gaagaaatat ctcgtcaagc ggctgctcct gctcatcccc acgttcatcg gcatcacgct
    11101 catcacctat ctcatggtgc gcctggcgcc gggcgactac accacgctgc gggccggcgt
    11161 gcagggcgag ctcaagcagg gcgccatcgg gaaggagatc atcgagcagg agaaaaagct
    11221 ctttggcctg gacaagccca tcccggtggg atacgcagag tggctgtgga agttcgtgcg
    11281 gctcgatttc gggatctcgc gcaaggacgg acggcccgta tcggcgcgca tcgcggaggc
    11341 gctgcccatc acgctccggt taaacctcat cgcgatgatt gtcgtatata tcatttcaat
    11401 tcccatgggg atcttctcgg ccgtgcgcaa ggactccacc tacgaccgcg tctcgagcct
    11461 tgcgctcttc gtgctctatt cgctaccgtc gttctgggtg gggctgctgc tgctcttggt
    11521 gttttccggg ggcgactatc tcaacctgtt cccgctcggc ggcgagatct ccgactgggc
    11581 gcgcgacttc ggatttttcg gcaggctggg gaacaggatc tggcatctgg tgctgcccgt
    11641 ggtcacgctc acctatggga gcttcgcctt cctctcgcgc tacgcgcgcg cgaacatgct
    11701 cgaggtcatc aaccagcaat acatcactac ggcgcgcgcc aaggggctct ccgataacag
    11761 cgtcgtgttc gtgcatgcct tccgcaattc gctggtgccg ctggtcacgc tcatggccac
    11821 gctcctcctg gggctcctgg gcgggagcgt gatcgtggag cagatcttct ccatcccggg
    11881 catggggctt ctggccttcg aggccatcct ctcgcgggac atcccggtga tcatggccat
    11941 cgcggccatc tcggcgctcc tgaccctgct ggggatactg ctggcggaca tcatgtacgg
    12001 cctggtggac ccgcgcattc ggctggaggc gaagctgtga gcgcgcaggg ctactggtcg
    12061 aatgtgtggc gggagttccg gcgcaaccgc ttcgcgctcg cggggctttc catcgtcgcc
    12121 atcctctttg tgatcgcgct gctcgcgccg cttatcgcca acgaccgtcc ctatgtcttc
    12181 gtggacgggg ggcgcgccta ttttccgctc tttaaggact atcccgcgct caaggacgtg
    12241 aatttccgga ccacccgcgg gtccttttcc gtcatgccgc ccattccgta ttcgtattcc
    12301 gcctacgacc tcgacgcgat cgtgctgccg ccctcggcgc gccatgtcct ggggaccgac
    12361 gagcagggcc gcgacctcgc cgcgcggatg gtgtacggta cgcgcgtgtc catcctggtg
    12421 ggctttatcg cggtgttcat ctatgtgagt atcgggatcg tcgtgggggc cgtcgccggc
    12481 tattacggcg gtcggacgga catgctcatc tcgcgcttca tcgagatcgt gatgtgcttc
    12541 ccaacctttt ttctcatact caccatcctc gcgctggtgg gcccgagcct caccagcgtg
    12601 atggtggtca tcggcctcac gggctggacg gggatcgcgc gcatcgtgcg cggcgagttc
    12661 ctgaaattgc gcgaggccga ttacgtgacc gcctcgcgcg cgctgggggc gggggacgcc
    12721 cgtattatta tgcggcatgt gctgcccaat gcgctcgcgc cggtgctggt gtcggccacc
    12781 ttcgggatcg cctccaccat cctcatcgag tcgtcgttga gcttcctcgg cttcggtgtg
    12841 cagccgccca cgcccagctg gggcgacatc ctgtcgcagt cgcgcgattt catggatttc
    12901 gcctggtggc tcacgctcat cccggggttc gcgatcttca tcaccatcac ctcgtacaac
    12961 ctggtggggg aggggttcca agacgccatt gaccccaagg cgctcaagcg ggggcgggat
    13021 taggggccgg ccatggacca gaagctcatc gaagaggtga tcaaccgctc catcaagaag
    13081 ctcaacgacg agtacgagct catcaacacc accttcctga acgtgctgca tgcgaacatc
    13141 gaccagctca tcgagaacac ggaggcttgg ttcggccccg ccgcgcccaa agagttcaag
    13201 gacgtgctca actcgctcat gacgctggcc tatctctact acgaggagcg gcgcgagtac
    13261 tcgaagtaca ttgtcgagga gaaatacgag aacgtcgacc tggccgagct gatggacgac
    13321 atcatcgaga aggtccggtt cctgtttccg gcgcgcgcgg tgacgctggt cgcggccgaa
    13381 tcggattgca gcatccgggc ctcgcgctcg ctggtgcggg agtccttctt taacatactc
    13441 ctgtcgctgt tccagttcat ggacgaacgc tcctcgtgcg gcatccggct cttcactgag
    13501 ggccagaacg tgaagatcgc gctggccttc gacaacctcg cggaggagc
//
LOCUS       DAORHF010000086        13479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3637_length_13479_cov_4.399732, whole genome shotgun sequence.
ACCESSION   DAORHF010000086 DAORHF010000000
VERSION     DAORHF010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3637_length_13479_cov_4.399732"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..691
                     /locus_tag="PKJ16_08855"
     CDS             107..691
                     /locus_tag="PKJ16_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV47138.1"
                     /translation="MSILLSRWRPDQIFADPFCGSGTIAVEAALIGRNIAPGINRSFA
                     AEDWPHLSRQLWMKAHDEARAAVRSDTLQILASDHDWSVFKMARENAERAGVAEAITF
                     QKKPLAEFSSKKRGGVIVCNPPYGERMGDTQAAERLYEEMGRIFPGLPGWAVYVLTAH
                     EEFEKFYGKKSDKNRKLYNGKIRCHLYQYTKDVG"
     gene            1196..2872
                     /locus_tag="PKJ16_08860"
     CDS             1196..2872
                     /locus_tag="PKJ16_08860"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HNV47139.1"
                     /translation="MQEVNFAAGKDGVFTLSAITPVALGDDRDTLTVRIVNRSPYPDD
                     KVFITVYGKNIKDNAYNYLAFGKDGNAVASEFEPVNPDKIDDPKAEMYSVALNQLTNA
                     GKDPDDKEKTIYSFQCPYKNNLVSGRIVFAFGKKLLGLGLTNSADKLSIREPSSTGAP
                     DYQTLFEFMELSVTRQDPTKAVPNPPYVLFINTSVVDFFSIGMQMSVEVQKPGKNAVT
                     KTVGFPDGARKDVFAAFKKAEGDDSLKKFCYYIQVEDKDKKKQKLRIMAPHQEIAVNS
                     GPDACKGINSKSALFDYLDVVIDEGWKNYSKKPLTIDDSVRVPPKNDIYAYGFRYKPE
                     LITAEGILKMTCVAWPENECGVDANGKPIRCPFPSDNEICSLPKITTREVFDCDDICY
                     GQRDPRPDFNDSYKNEGSNGHKRLVALIAAALNRGVFENYADWGNPDKYYKRADKKYN
                     FYSKVMHDNALEVADEKYPDYKVRRVYAFGYDDIYGQDPTIAADVPTVNNVTITIPKF
                     DLNVDTESQVSANIRSTLGKRTTLYVALGAGALAIGLIVVVNIIRRRRAA"
     gene            2975..3454
                     /locus_tag="PKJ16_08865"
     CDS             2975..3454
                     /locus_tag="PKJ16_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV47140.1"
                     /translation="MKKLRVYLDTSVISFIFADDAPEYQSVTIDFFDNFLQEYDVYIS
                     EIVYLEINKTGDVARKNQLLGAIENFDVRVYDAVNDEIESLAEVYVRSGVIPRNKFED
                     AMHIAFCTYYDFDVLLSWNFRHLANIRKQIEINSINKAQGYLKDLNLLNPMEVIYEK"
     gene            3444..3608
                     /locus_tag="PKJ16_08870"
     CDS             3444..3608
                     /locus_tag="PKJ16_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47141.1"
                     /translation="MKNNYDKYMKEVWDMKDKAYEDFVASGYSTYADYLKNELKNVKI
                     TFESKVSVTK"
     gene            4044..5738
                     /locus_tag="PKJ16_08875"
     CDS             4044..5738
                     /locus_tag="PKJ16_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47142.1"
                     /translation="MKQSSLFVVLMFALILACAGGKDSGDGAGEGESDGSGSVNAPAA
                     ITDQPTIGGAVIGLDGVVILQNNGGYDLSVTADGPYVFDMKPKKGDAYAVTVKYQSQG
                     NYCCVNNGNGAVGDSAVTNVNVYSLRKVYPHDGVANLNFGNSISISGGTVVAGAEWGE
                     GVNSHPGLAYVFVKDGSAWTQEARLAASDGTNANRFGCSVSLDGDTVIVGANCDSAKG
                     TASGAAYVFKRSGTSWTQEAKLVASDGAIGNFFGGSVSLSGDIAIIGAYRNNAKGDSS
                     GAAYVFRRTSSGWTQEAKLVASDGAANDYFGLSVSVNGAIIVVGATGDDDKGSGSGSA
                     YVFTYANKAWTQEAKLTASDGVAGASFGRSVTVSGDTALVGADNMDSWKGAAYAFRRS
                     FNKTTNKYAWRQEAKLVASDGASGNYTGDYLGWSVSLSGNMAVVGSYHCDTRAEASGA
                     AYLFRRYTDKSGNTLKWVQKAKLSASDGAAYDYFGIDVSISGETIAMGSLFNNTRGSK
                     SGAAYILNSTTPSSLGANIRSMLGRRTTLFIAIGAGVLAIGIIGAVAVIRRRRKSA"
     gene            5951..6556
                     /locus_tag="PKJ16_08880"
     CDS             5951..6556
                     /locus_tag="PKJ16_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47143.1"
                     /translation="MKRIVLYLIPAILASSLFSGCMGFQSGQARIIDPYPPKNFMTKK
                     KPKLVSVKVNYPVDNSNVQGAVNASMAKTTTGYIMDVSEDVFDDSKYFKYAAPGKPSD
                     CTLDIHIKYKANLNNCLNIVSGLTLLIVPTIQSEEFTATCKLYGRGRRYLGQKVIHQK
                     LTSVYQTLLIVGMPFASVGTVEKRMWRRIVRDASVWMRDKI"
     gene            6753..7019
                     /locus_tag="PKJ16_08885"
     CDS             6753..7019
                     /locus_tag="PKJ16_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HNV47144.1"
                     /translation="MKWKIARAKLQFSELIRMVYKEPQLIFNRETMVAAIIGPEEYRE
                     YLAMKEQSSRRSLASAFEELRSICADDSYELIVPARSDRENSFP"
     gene            7019..7414
                     /locus_tag="PKJ16_08890"
     CDS             7019..7414
                     /locus_tag="PKJ16_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017713128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV47145.1"
                     /translation="MQYLCDTNIISELAKGEPNPGVIEWAGTVAGCALSVITIEELHY
                     GLSWRPNTRVQAWVEGFVRDHAEVLPVTEPIARAAGVLRGRMRAGGITRTQADMLIAA
                     TALVHGLTVVTRNGRDFEGCGVPVLDPFR"
     gene            7491..9527
                     /locus_tag="PKJ16_08895"
     CDS             7491..9527
                     /locus_tag="PKJ16_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV47146.1"
                     /translation="MHAILITALIGFAPVPVIYFFYLYYFSIKRDVLHHLEYFAYGML
                     LAPLVLFAGPYLVALVPAKSVLAQGFLQIALVEKAGAFAVVFLLMRRSRARLTVSNAV
                     ACAMFLGMGFAAVENVVFAATASLSVLLVRFMSAVPLHVLSCGLIGYYLALRELSSSR
                     THRAAYFAVAVAVPVFFHGMYDSLILLGGSKTFLTAGLLFLLMALMELNLAKSQVLPL
                     LEGLAEERLSLEDWQTIERQQQFERWIQLSMGARNVQAVPFFRYVLSRVKMVVVAGIV
                     AFAAWFLIKGGWLVSVLGVPLTGQESLVVFVLIPVLYAAQLVLVGVVNPDYFKYSVIR
                     IPVIIDTVMSLDVDAAREDDVVDAVTYHITGLNAFVKTAAPLAPGTRVRCILYCSRFS
                     SPALECEVAWDHHAGPEPLNGTLVRFMARPAGFWVFLARYYFFRVSQGIVFALRLPGY
                     RAIRRHFVRPVSVMQDEAHFPAGHVLFRQGDPGDRFYLVLRGMVDIYKTFRNGRRVLM
                     TTLTRGDIFGEMALVGNQPRLATAECRTDCLVAIADPDNLDALIRGNPDFTRTLIETF
                     AARFHETDAAMFKNLRQMNRSMRADRRFLGSLARLLLSGAYADPDGDGATVAIDIDGR
                     ARALGCDREKVRALLRVVNESENEEELINGVDSALRDRIVKAFGAGRIELREKR"
     gene            9542..9892
                     /locus_tag="PKJ16_08900"
     CDS             9542..9892
                     /locus_tag="PKJ16_08900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47147.1"
                     /translation="MPAFQYNSTVKDGVTIVAIAGSLDVVSAKQLDAKLSADIAKGAR
                     RVVINLSKVDYIASAGLGIIVGANAALKKQGGEVRLSALPDKIAKIFELLSFSKLFKI
                     HKTDTEALASLSGK"
     gene            complement(9907..10152)
                     /locus_tag="PKJ16_08905"
     CDS             complement(9907..10152)
                     /locus_tag="PKJ16_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HNV47148.1"
                     /translation="MQDIETTATDASGDNPRRLAHDLNNAMASIIGSVDLANLKVPAD
                     DPARRYLEQIGSSAERAAGLIQRLIVILRRDYPDDPK"
     gene            complement(10230..10439)
                     /locus_tag="PKJ16_08910"
     CDS             complement(10230..10439)
                     /locus_tag="PKJ16_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008002189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HNV47149.1"
                     /translation="MRTTITIEGMSCSHCEMRVKRALEEIAGVRSARVSHETKSAIVE
                     TDDDVDAALLERAVTGAGYTVTGTS"
     gene            10600..10977
                     /locus_tag="PKJ16_08915"
     CDS             10600..10977
                     /locus_tag="PKJ16_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47150.1"
                     /translation="MAAAALIALVSGQLLAQRGPGRGPGMGMCPGMGGGPHGFAHLDR
                     MQKELGLTDAQVKKIFDIGTEYRQKYFDNRGNRDKVMALHEEHRKAVESVLTKEQREK
                     YDKLFKDGRMRGKGPGKGMGRGR"
     assembly_gap    11101..11200
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            11322..11798
                     /locus_tag="PKJ16_08920"
     CDS             11322..11798
                     /locus_tag="PKJ16_08920"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HNV47151.1"
                     /translation="MRRTLTITILSMLLCMVAIDAGAQMHDRGRGPRHGHGPGGPGPG
                     GEGGCFGDPAFLRDALKLDEGQIEAVGRINREHRDELRALKERLRPLQRDLRALLLGD
                     PVDMEKVRAMLRSVADVEIELRLARIKHRLAIEKVFTPEQREKHRREMKKRMRADD"
     gene            11798..12313
                     /locus_tag="PKJ16_08925"
     CDS             11798..12313
                     /locus_tag="PKJ16_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV47152.1"
                     /translation="MDEREFAAVIGETKGVVLSAIERHLAPRFSAAIDDVVQEAYLRA
                     YRSLTAGRFRGDSAVGTWLYAIARNEALRMNERLAREERKLLRSMERDAGDDVMRDDG
                     VSAEIDALYDAIARLPEKYRAVLERVARGWPVRDIARELGIETGTVKSRASRGREMLH
                     RLMREDAYGRE"
     gene            12300..12665
                     /locus_tag="PKJ16_08930"
     CDS             12300..12665
                     /locus_tag="PKJ16_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47153.1"
                     /translation="MVGNNHAERLHAQVRERLSNADWDRRMATAIVAKRRTRSSRIAA
                     SSVASLAVAALVVVAFMFRGGEPAEPRYESFISTQLNGTHSAVFHAADAGPRSASVRA
                     DVLVDSGIDAVIDDTLAMR"
     gene            complement(12668..>13479)
                     /locus_tag="PKJ16_08935"
     CDS             complement(12668..>13479)
                     /locus_tag="PKJ16_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV47154.1"
                     /translation="PSVVLVSNALLLDELAREHIVALPIDEVYLSWDDDINAAAGAIR
                     RGQRATSFRSALEGLVETKKSRGGDRPLIGLQIVATRSTVGHVREIAAYAIGAGAERI
                     VLSNLFPYTAGMSEEILYVPGGKPKTDLALAMRGLRAPKGIVIASQRADAPRRCPFVE
                     RGTAFISSSGAVSPCPELAYTHPAYYFGAPRTHRAFTLGEIGRERLGGIWRSERFVRF
                     RESFGYFEFPDCTACVDPERCWHRTATACDCYGNPVPCGECLWAKGIVVCP"
BASE COUNT         2724 a         3742 c         4236 g         2677 t
ORIGIN      
        1 caggccgccg ccgccgtatc gccggcgcag gcccagcagc tcgggataga accacgcgtt
       61 gtcctggatc gaggtcacgc cgagcgacgc gaaggcctcg agggcgatgt ccatcctcct
      121 ctcgcgctgg cggccggacc agatcttcgc cgatcccttc tgcggctcgg ggaccatcgc
      181 cgtcgaggcc gcgctcatcg ggcgaaacat cgcgccgggc atcaaccgct cgttcgcggc
      241 cgaggactgg ccgcatcttt cgcggcagct ctggatgaag gcgcacgacg aggcgcgcgc
      301 ggcggtgcgg agcgacacgc tccagatcct cgcctccgac cacgattgga gcgtgttcaa
      361 gatggcgcgc gagaacgccg agcgcgcggg cgtggccgag gcgatcacct tccagaagaa
      421 gccgctcgcg gagtttagct cgaaaaagcg cggcggcgtg atcgtatgca acccccccta
      481 cggcgagcgc atgggcgaca cgcaggcggc ggagcgcctc tacgaggaga tggggcggat
      541 cttccccggc cttcccggct gggccgtcta tgtcctcacc gcgcacgagg agttcgagaa
      601 attctacggg aagaaatcgg acaagaaccg caaactctat aatgggaaga tcaggtgcca
      661 tttgtatcag tatacgaaag atgtcggttg acacgaggat ggcattcgcg atgatgcaac
      721 caccgcgtgc ggtccattgc tcgttgtcgt ttcacatgcg ggctcccggt tcggattggg
      781 aaggcggaaa aaaaacacat cacagaggag cggattggtt atgataaagc atgcactctc
      841 acgaacgttt cgaaaaaaca cgatctcggc gcgcctatta ctcgcggcgg tcctggccat
      901 gtcgcttgcg ggacttgccg cctgcggcgg cggcatggag aatgaaccgg taatcggcgg
      961 cggccccaat acgcttcgca tgatggggtc gcaggctgat ttgcactatc cgggagctgg
     1021 tggcgggaag gtggagcacg tcgtgtgcac cggctatgat gcggatggga acgtggtgta
     1081 ccgctccggc gaggagccgt acgacggcga catcaccttt tcgggcattc cggacaagac
     1141 cgttaaagtg cagatcgatt acaaccgcgg gaccgggtac acgctggccc ggcacgtgca
     1201 ggaggtcaat ttcgcggccg gcaaggacgg cgtgttcacg ctcagcgcta tcaccccggt
     1261 ggccctgggg gacgatcgcg acacgctcac cgtgcggatc gtcaaccgtt ccccgtaccc
     1321 cgatgacaag gtttttatca cggtgtacgg gaagaacatc aaggataatg cgtataacta
     1381 tcttgcgttc ggtaaagatg gcaatgccgt cgcctccgag ttcgaaccgg tcaaccctga
     1441 taaaattgac gatccgaaag ccgaaatgta ttccgtggcg ttgaaccaat tgacaaatgc
     1501 cgggaaagat cccgacgaca aggaaaaaac catctattcg tttcagtgtc cctataagaa
     1561 caatctcgtt tcgggccgta tcgtctttgc gttcgggaaa aaactccttg ggcttggatt
     1621 gaccaattcg gccgacaagc tttcgatccg tgagccatcg agtacgggcg ctcccgacta
     1681 tcaaaccctg ttcgaattca tggaattgtc ggtgacgcgg caggatccta ctaaagccgt
     1741 gccgaacccg ccctatgtgt tgtttattaa caccagcgtc gtggattttt tctcgattgg
     1801 catgcaaatg tcggtggagg ttcaaaagcc cgggaaaaat gcggtaacaa aaaccgttgg
     1861 cttccctgat ggcgcacgaa aggacgtgtt cgctgccttc aaaaaggcgg aaggggacga
     1921 ttcgttaaaa aaattctgtt attacattca agttgaagat aaagacaaaa aaaagcaaaa
     1981 actgcgcatc atggcccccc accaggaaat agccgtgaac tccggtcctg atgcctgtaa
     2041 gggaataaac agcaaatcgg cgctgtttga ttacctcgat gttgttattg atgagggctg
     2101 gaagaattat tccaaaaaac cgctcacgat agacgattcg gtgcgggtcc cgccaaagaa
     2161 cgacatatat gcatacggct tccggtataa accggagctc atcacggcgg aaggcatatt
     2221 aaagatgacg tgcgttgcat ggcctgaaaa cgagtgtggc gtggatgcga atggcaagcc
     2281 gatacgatgc ccgttcccga gtgataatga gatctgttcg cttccgaaaa taaccacccg
     2341 cgaggttttc gactgcgatg atatttgcta tggccagaga gacccgaggc cggatttcaa
     2401 tgactcgtac aagaatgaag ggagcaacgg gcacaagcgt ttggtcgcgc tgattgcggc
     2461 ggcgctcaat cgcggcgtgt tcgaaaacta cgccgactgg gggaacccgg acaagtacta
     2521 taagcgggcc gataagaaat acaatttcta ctccaaggtg atgcacgata acgctctcga
     2581 ggtcgcggac gaaaagtatc ccgactacaa ggtccgcagg gtctacgcgt tcgggtatga
     2641 tgatatttac gggcaggatc cgacaattgc cgcggatgtc ccaacggtca acaacgtcac
     2701 catcaccatc ccgaagttcg atttgaacgt cgatacggaa tcccaggttt ccgcgaacat
     2761 ccgctccacg ctgggcaagc gcaccacgct ctacgtcgcg ctcggcgcgg gggcgctggc
     2821 gatcgggctc atcgtggtcg tgaatatcat ccggcggagg cgggcggcgt aggagtgcac
     2881 atggttgaat caccattttg tcttttggtt ccgggtgaat tgcatgggga aatacattga
     2941 caagtccact ggcggttgat gcagtattgt cgccatgaaa aagctaagag tatatctcga
     3001 cacatcggtc atcagtttta tattcgctga tgatgcgccg gaatatcaat cggtaactat
     3061 tgattttttt gataatttcc tgcaagagta tgatgtttat atatcggaaa tcgtgtatct
     3121 tgaaataaat aagaccgggg atgttgcgag aaagaaccaa ctcctcgggg cgatagaaaa
     3181 ctttgatgtg agggtgtatg atgcggttaa tgatgaaatt gaaagcttgg ccgaggtgta
     3241 tgtccggtcg ggggttattc cgaggaataa gttcgaagat gcgatgcaca tcgctttctg
     3301 cacctactat gatttcgatg tgctcttgag ttggaatttc aggcaccttg ccaatatcag
     3361 gaagcagatc gagatcaatt caatcaacaa ggcgcaggga tatctcaagg atctcaatct
     3421 gcttaatccg atggaggtta tttatgaaaa ataattatga caaatacatg aaagaagtct
     3481 gggatatgaa agataaggcg tatgaggatt ttgttgccag cggttattca acatacgcag
     3541 actatctgaa aaacgaactc aagaatgtaa aaatcacctt tgagagcaag gtttccgtga
     3601 caaaataata cgtccactct cctggtgcag tagcgctggt cgtcggactg attgtcggga
     3661 tccatatcat ccggcggagg cgggcggcgt agcggttccg ttgcggcaga acgatgcgaa
     3721 tggtgaaaag ggcgcccatg gtaacatggg cgctctcatt tttcggggtc tgatccggcc
     3781 tatcgattgt gccgatgggg cggatctcca tcaaaatcta ccaaggccca tcccatgtcg
     3841 gatgacgttc cgttggccgt ctgaatagag gttgaatagg ggctgatccg aatcgaagag
     3901 ctggatagcc cgtgactcca cgggtattcg cttttttaga ggggcttttt cgcttgctat
     3961 ttcggggata atatctgtta ataaacattc aatagataaa tgtatatatc aattaatgta
     4021 tagactctcg tccgggggga accatgaaac aatcatcgct cttcgttgtc ttgatgttcg
     4081 cgctcatcct cgcctgcgcc ggcggaaagg acagcggtga tggcgctggt gaaggagaga
     4141 gtgacgggtc cggttccgtg aatgcgccgg ccgctatcac cgatcagccg accatcggcg
     4201 gtgcggtcat cggcctggac ggggtcgtaa ttcttcagaa caatggcgga tacgatttaa
     4261 gcgtaaccgc cgacgggccg tatgtgttcg atatgaagcc gaagaagggc gacgcgtatg
     4321 cggtgaccgt taaataccag tcgcagggga actactgttg cgtcaacaat ggcaatggcg
     4381 cggttggtga ttccgctgtc acgaatgtca acgtgtactc actcaggaaa gtctatcccc
     4441 acgatggggt ggccaatctc aacttcggta attccatttc aattagcggc ggcacggtcg
     4501 tggccggagc ggaatggggg gagggcgtta actcgcatcc cggccttgcg tatgtttttg
     4561 tcaaggacgg atcggcgtgg acgcaggagg ccaggctcgc ggcatccgac ggcaccaatg
     4621 cgaacagatt cggctgttcc gtttcgcttg atggcgatac cgtcatcgtt ggcgcaaatt
     4681 gcgacagtgc gaagggcacc gcgtccggcg ccgcttatgt gttcaagcga agcggtacgt
     4741 cctggactca ggaggccaaa ctggtcgctt cggacggcgc aatcggaaat tttttcggag
     4801 gttcggtatc cttgagcggc gatatcgcga tcatcggcgc gtatcgtaac aacgccaaag
     4861 gcgatagttc cggtgcggcg tatgtattca ggagaaccag ctcgggttgg acacaggaag
     4921 cgaaactcgt cgcttcggac ggcgccgcca acgattattt tgggctttcg gtatccgtga
     4981 atggcgctat catagtcgtc ggcgcgaccg gagacgacga caagggaagc ggttccggct
     5041 cggcatatgt cttcacgtat gccaataagg cctggacgca ggaggcgaaa ctcacggcct
     5101 cggatggtgt tgccggcgcg tcattcgggc gctcggtaac cgtgagcggc gacaccgctc
     5161 tcgtgggggc ggacaacatg gacagctgga agggcgccgc gtatgcattc aggagatcgt
     5221 tcaataaaac gacgaacaaa tatgcctgga ggcaggaggc gaagctcgtc gcctccgacg
     5281 gcgcgtccgg aaactacacc ggcgattatc tcggatggtc ggtatcgctc agcggcaata
     5341 tggcggtggt cggttcgtac cattgcgaca ctcgggctga agcgtcgggc gccgcgtatc
     5401 tatttagacg ttacacggat aaatcgggga ataccctgaa gtgggtgcaa aaggcgaaac
     5461 tttccgcatc ggacggcgcg gcgtatgatt atttcgggat agacgtatcc ataagcggcg
     5521 aaacgatcgc gatgggttcc ctgtttaaca acaccagagg ctctaaatcc ggggcggcgt
     5581 atattctcaa tagtacgacg ccatcttccc tcggcgccaa catccgctcc atgctgggca
     5641 ggcgcaccac gcttttcatc gcgatcggcg cgggggtgtt ggccattggc atcatcgggg
     5701 cggtggcggt catccggcgg aggagaaagt cggcgtaatg gaattatcgt agtaccagcc
     5761 attcttgagc attgcgcagg gctcattgat tgaacgataa gaacgaagta cgggaagttg
     5821 tctgcggtga agccctctcc tttaggagtc cccaaagggg aggcttcgcg cagggcgcac
     5881 cggaggcggg gtgaggttgt tcgcgataca ccgcacctaa cgtcaatgtg aagtataacg
     5941 ggagcatgcc atgaaaagaa tagtattgta tcttattccg gcgatcctcg catcatcgct
     6001 tttctccggg tgcatgggat ttcagtccgg ccaggcgcgg atcatcgatc cctatccccc
     6061 gaagaacttc atgacgaaaa agaaaccgaa gcttgtctcg gtcaaggtga actatccggt
     6121 cgacaacagc aatgtccagg gggcggtgaa cgcgtcgatg gcgaagacga ccaccggcta
     6181 catcatggac gtctcggagg acgtcttcga cgacagcaag tattttaagt acgcggcccc
     6241 cggcaaaccg tcggactgca cgctggacat tcacatcaaa tacaaggcga acctgaacaa
     6301 ctgcctgaac atcgtgagcg gcctcacgct cctgatcgtc cccacgatcc agagcgagga
     6361 gttcacggcc acttgcaagc tctatggcag gggacggcgc tacctggggc aaaaggtgat
     6421 ccaccagaag ctgacctcgg tataccagac cctgcttatc gttggcatgc cgttcgcgtc
     6481 ggtgggcacg gtcgagaagc ggatgtggcg ccggatcgtg cgggacgcgt cggtctggat
     6541 gcgggacaag atttaaaagt caaagcatgt ttaattacga ttgggggctc ccgtgaaaat
     6601 tcatgaaagc cccttttttg tatgataagg cgttggttct gtagagcctg tgagttgccg
     6661 tcgatattcg aaaaaattta ctataatcgc cttgacgcgt tcaccaaata tgactatatt
     6721 gtctatgtga tgtggtcatg tttggtgatg atatgaaatg gaaaatagca cgcgcgaaat
     6781 tacaattttc cgagctcatc cgtatggttt acaaggagcc acaattgata ttcaatcgtg
     6841 agaccatggt ggcggcgatt atcggacctg aggaataccg tgagtatctt gccatgaaag
     6901 agcagtcatc ccggcgctct ctggcatcgg cattcgagga attgcgatcc atctgtgccg
     6961 atgattcgta tgaactgatc gtgcccgctc gaagcgatcg agagaatagt tttccctgat
     7021 gcagtacctc tgcgacacca acattatcag cgaactcgcg aaaggcgagc ccaacccggg
     7081 cgtaatcgaa tgggcgggta cggttgcggg atgcgccctg agcgtgatta ccatagagga
     7141 gctgcactat gggttgtcat ggcgtcccaa tacgcgggtc caggcgtggg tcgagggatt
     7201 cgtccgggac cacgccgagg tgttaccggt cacggagccc atcgcccgcg ctgcgggagt
     7261 gcttcgcggt aggatgcggg ccggcggcat cacgcgcaca caggccgaca tgctcatcgc
     7321 cgcaacggcg ctggtgcacg gcctcacggt tgtcacccgc aatgggcgtg atttcgaagg
     7381 gtgcggtgtc ccggtgctcg atccgttccg gtagggagta atccgtaaaa aagcttgcac
     7441 tgcgcgggga aacgagtata ttcatggaac catagtaggg gaatagcgcc atgcacgcca
     7501 tcctcataac cgccctcatc ggattcgcgc ccgtcccggt catctatttc ttctacctgt
     7561 actatttttc catcaaacgc gacgtattgc accatctgga gtacttcgcg tatggcatgc
     7621 tgctggcgcc gctggtcctc ttcgccgggc cttatctggt cgcgctggtg ccggcgaaga
     7681 gcgtgctcgc gcagggcttc ctgcagatcg ccctggtcga gaaggccggc gccttcgccg
     7741 tcgtcttcct gctcatgcgc cgctcccgtg cgcgcctcac ggtgagcaat gccgtcgcat
     7801 gcgcgatgtt tctgggcatg ggcttcgcgg ccgtcgagaa cgtcgtgttc gcggccaccg
     7861 cgtcgctctc ggtgctcctg gtgcgcttca tgagcgcagt gccgctgcac gtgctctcct
     7921 gcgggctcat cggctactac ctcgcgctcc gggagctctc ctcgtcgcgc acgcaccggg
     7981 ccgcgtattt cgccgtggcc gtggcggtac cggtcttctt ccacggcatg tacgattcgc
     8041 tcatcctgct gggcgggagc aagaccttcc tcaccgcggg gctcctcttt ttgttgatgg
     8101 cgctgatgga gctcaacctc gcgaaatcgc aggtgctccc gctgctcgag ggcctggccg
     8161 aggagcggct ctcgctcgag gactggcaga ccatcgagcg gcagcagcag ttcgagcgct
     8221 ggatccagct ctcgatgggc gcgcgcaacg tgcaggcggt cccctttttc cggtacgtgc
     8281 tgtcccgggt gaagatggtc gtggtggcgg gcatcgtcgc cttcgcggcc tggtttctca
     8341 tcaagggcgg ctggctggtg tcggtgctgg gcgtgccgct cacgggccag gagtcgctgg
     8401 tggtgttcgt gctcatcccc gtgctgtacg cggcgcagct cgtgctggtc ggcgtcgtga
     8461 acccggacta cttcaaatac agcgtgatac ggattcccgt gatcatcgac acggtgatgt
     8521 cgctcgacgt cgacgccgcg cgcgaggacg acgtggtgga cgccgtcacc tatcacatca
     8581 ccggcttgaa cgcattcgtg aagaccgccg ccccgctcgc ccccgggacc cgggtccggt
     8641 gcatcctgta ctgctcccgc ttctcgtcgc cggcgctgga gtgcgaggtg gcctgggacc
     8701 atcacgcggg gcccgagccg ctcaacggga cgctcgtgcg ctttatggcg cggcccgcgg
     8761 gcttctgggt gtttttggcg cgctattatt tcttccgcgt ctcgcaggga atcgtcttcg
     8821 ccctgcgcct gcccggctat cgcgccatcc ggcggcattt cgtccgtccc gtgtcggtga
     8881 tgcaggacga ggcgcacttc cccgctggcc atgttctttt ccggcagggc gatcccggcg
     8941 accggttcta tctcgtcctg cgcggcatgg tggacatcta caagaccttc cggaacggac
     9001 ggcgggtgct catgaccacg ctcacccgcg gcgacatctt cggggagatg gccctggtgg
     9061 gcaaccagcc ccgcctggcc acggcggagt gccgcacgga ctgcctggtg gccatcgccg
     9121 atcccgacaa tctcgacgcc ctcatccggg gcaacccgga cttcacgcgc acgctcatcg
     9181 agaccttcgc cgcgcgcttt cacgagaccg acgccgcgat gttcaagaac ctgcgccaga
     9241 tgaaccgctc gatgcgcgcc gaccggcgct ttctgggctc gctggcgcgg ctgctcctct
     9301 ccggcgcgta tgccgatccg gacggcgacg gcgccaccgt cgcgatcgac atcgatggcc
     9361 gtgcccgtgc gctggggtgc gaccgcgaga aggttcgcgc gctgttgcgc gtcgtgaacg
     9421 agagcgaaaa cgaggaagag ctcataaatg gtgttgacag cgcgctccgg gaccgtatcg
     9481 tgaaggcctt cggggcgggc cgtatcgagc tccgggaaaa acgatagcgg acggagcggg
     9541 catgccggcg tttcagtaca actccacggt aaaggacggc gtgacgatcg tcgcgatcgc
     9601 cgggagcctc gatgtcgtct cagcgaagca gctcgacgcg aagctctcgg ccgacatcgc
     9661 gaagggcgcg cgccgcgtcg tcatcaacct ctcgaaagtg gactacatcg ccagcgccgg
     9721 gctgggcatc atcgtgggcg ccaacgccgc gctcaagaag cagggaggcg aggtgcgcct
     9781 ctcggcgctc cccgacaaga tcgcgaagat cttcgagctc ctgagcttct ccaagctgtt
     9841 taaaatccac aagaccgaca ctgaggcgct tgcgtcactc agcggcaagt gatgctcccg
     9901 cggggctcac ttcgggtcat cggggtagtc gcggcgcagg atgacgatga ggcgctggat
     9961 gaggccggcc gcgcgctcgg ccgaggagcc gatctgttcg aggtacctgc gcgcgggatc
    10021 gtccgcgggg accttgagat tggccaggtc caccgatccg ataatggacg ccatcgcatt
    10081 gttcaggtcg tgcgccagcc tgcggggatt gtcgcccgac gcgtcggtcg cggtagtctc
    10141 gatatcctgc atggcccctc gtgtgtgcgg gatgtgtgat gggtcgatgg tgccggcaaa
    10201 ccgttttgtt tcaagaacat tttacccggc taggaggttc ccgtcaccgt gtagcccgcg
    10261 cccgttaccg cgcgttcgag gagcgcggca tcgacgtcgt catcggtctc gacgatcgcg
    10321 ctctttgtct cgtgcgacac ccgcgccgag cgcacgcccg cgatctcctc gagcgctcgc
    10381 ttcacgcgca tctcgcaatg agagcacgac attccttcaa tggtaatcgt tgtgcgcatg
    10441 tccatcacct cctcgatgcc gtatacgtat agcgccccgg tcggcgtggc gcaaaaaaac
    10501 tggaaccggt tttgcggaac cggggtcaaa cgggcatctt gtacgatgag gtatgcaaac
    10561 atggcaaatc acaaaagcat ttctaaagga gtggcgatcg tggccgctgc cgctctcatt
    10621 gcgctcgttt cggggcaatt gctcgcccag cggggacccg ggcgcggacc gggcatgggg
    10681 atgtgcccgg gaatgggcgg cggtccgcat ggattcgcgc atctggaccg catgcaaaag
    10741 gagctcgggc tcaccgatgc gcaggtgaaa aaaatcttcg atatcggcac ggagtaccgc
    10801 cagaagtatt tcgacaatcg cggcaaccgc gacaaggtga tggcgctgca cgaggagcac
    10861 cggaaggccg tggaatccgt attgacgaag gagcagaggg agaagtacga caagctcttc
    10921 aaggacggtc ggatgcgcgg caagggcccg gggaagggca tgggtcgggg gcgttgatag
    10981 cgccttatga aatcgccggt gcgatcgatg ggtcggtcgc accgggcgcg aaggggctct
    11041 gaccccgcga aggggctctg accccgcgaa ggggctctga ccccgcgaag gggctctgac
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cccgcggcat gatgaaaccg
    11221 catctgcaaa tgcaacattt ttattgtcgc aatccgctgg aatcgtgata ctcgtcatgc
    11281 agctgcttgt agattcaact ggctcggtcc gagggggggc gatgagaaga acgctaacga
    11341 taaccatcct ttcgatgctg ttgtgcatgg ttgccataga cgccggtgcg cagatgcacg
    11401 atcgcggcag gggaccgcgc cacggtcacg gacccggcgg accgggtccg ggcggggagg
    11461 gcggctgttt cggcgatccc gcgtttctcc gcgacgcgct caagctcgac gaggggcaga
    11521 tcgaagccgt cggccgtatc aaccgagaac accgagacga gctgcgcgcg ctcaaggagc
    11581 gcctgcggcc actccagcgg gatcttcgcg cgctgctgtt gggcgatccg gtggacatgg
    11641 agaaggttcg ggcgatgctg cggagcgtcg cggacgtgga gatcgagctg cgcctggcgc
    11701 ggatcaagca ccggctggcc atcgagaagg tgttcacgcc cgagcagcgc gagaagcacc
    11761 gacgggagat gaagaagcgg atgcgggcgg atgattgatg gacgagcgcg agtttgccgc
    11821 cgtgatcggc gagacgaaag gcgtcgtgct ctcggcgatc gagcggcacc tcgccccgcg
    11881 cttttcggcg gcgatcgacg acgtggtcca ggaggcctat cttcgggcct accgaagcct
    11941 gacggccggc cgcttccggg gggattccgc ggtggggacg tggctctatg cgatcgcgcg
    12001 caacgaggcg ctccgcatga acgagcggct ggctcgcgag gagcgtaagc tgctcaggtc
    12061 gatggagcgc gatgcggggg atgacgttat gcgcgatgac ggagtcagcg ccgagattga
    12121 cgcgctctac gacgcgatcg cgcggctgcc ggagaagtat cgcgcggtgc tggagcgcgt
    12181 ggcgcgggga tggccggtgc gcgatatcgc gcgcgagctt gggatcgaga cgggcaccgt
    12241 gaagtcgcgc gcgtcgcgcg gacgagagat gctacaccgt ctcatgaggg aggacgccta
    12301 tggtcgggaa taatcatgcg gaacgattgc atgcgcaggt gcgcgagcgc ctgtcgaacg
    12361 ccgactggga ccggcgcatg gcgacggcga tcgtcgccaa acggcggacg cgatcgtcgc
    12421 ggatcgccgc gtcgtcggtc gcctcgctgg ccgttgccgc gcttgttgtg gttgcgttca
    12481 tgttccgggg cggggagccg gccgaaccgc gatacgagtc gtttatctcg acgcagttga
    12541 acgggacgca ttcggcggtg tttcatgcgg cagacgcggg accgcgatcg gcgtccgttc
    12601 gcgccgacgt gcttgtcgac agcgggattg acgcggtgat cgacgacacg cttgcgatgc
    12661 ggtgacgtca cgggcacacg acaatgccct tcgcccagag gcactccccg caggggaccg
    12721 ggttgccata acagtcgcat gccgtcgcgg tccggtgcca gcagcgctcc ggatcgacgc
    12781 acgcggtgca gtccggaaac tcgaaatagc cgaatgactc cctgaagcgg acaaaccgct
    12841 ccgaacgcca gatgccgccc aatcgctccc gtccaatctc gcccaacgtg aatgcgcggt
    12901 gcgtccgcgg cgcgccgaaa tagtacgcgg ggtgcgtgta ggcgagctcc gggcagggcg
    12961 acaccgcgcc ggaggaggag atgaacgccg tgccgcgctc gacgaacggg cagcggcgcg
    13021 gcgcgtccgc gcgctggctg gcgatcacga ttcccttcgg cgcgcgcagc ccgcgcatcg
    13081 cgagcgccag gtcggtcttc ggcttcccgc cgggaacgta cagtatttct tcggacatcc
    13141 cggcggtgta cggaaacagg ttcgacagta cgatccgctc cgcgcccgcc ccgatcgcat
    13201 aggccgcgat ctcgcgaaca tgtcccacgg tgcttcgggt cgcgacgatc tgcaggccga
    13261 tgagcggccg gtcgcccccg cggctttttt tcgtttcgac aagcccctcg agggcgctgc
    13321 gaaacgaggt cgcgcgttgg ccccgacgta tcgcgcccgc cgcggcgttg atgtcgtcgt
    13381 cccacgagag gtacacctcg tcgatgggca gcgcgacgat gtgttcccgg gcgagctcgt
    13441 ccagaagaag cgcattgctc acgagcacca ccgagggcg
//
LOCUS       DAORHF010000087        13316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3706_length_13316_cov_4.955886, whole genome shotgun sequence.
ACCESSION   DAORHF010000087 DAORHF010000000
VERSION     DAORHF010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13316
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3706_length_13316_cov_4.955886"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="PKJ16_08940"
     CDS             complement(<1..2356)
                     /locus_tag="PKJ16_08940"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV47155.1"
                     /translation="MRVATKQAFIVPMRVLPAAMHARLDRIELIFQSMYMGLLLGMVA
                     YNLFVFISLRDVNYLYYVLFMLGYLILSVSFTGLGMQYLFPEGAWLGRTLPFAMAYTT
                     VFFALFARSYLKMDVQFPRIDFALRIVAALYVVLAALSIVAPDIMLNAVMLVHMPGAC
                     FFMAIGVMCAMKGRREAWYYIGAQTLMIIGVLLSSVVAFNLVPHGIVTYYGWEISNAI
                     QAVIFSLGLADRINVMKNELQVLNVDLENRVDERTRELSLANEKLTEMDRIKTNFFAN
                     VSHEIRTPLTLILSPVESVLQGDYGKPVGEDFFRSIQRNGVRLLRLINNLLDFSKIEA
                     GRMMLRVREVDAAALVRLHCGVVQSAAEARGLALSLDLPDRPIPLMLDAEKFERILMN
                     LLSNAFKFTEAGAITVRVSADDAVCTIEIADTGVGIPFEKLGSIFDRFSQVDSGSTRR
                     YEGSGIGLALAKEFVELHGGAITVDSRHGDAHAGDHGTTFTVRIPMGRRHLAGRADVE
                     FAGDEEGDEAPAGIIATIARLDDGSEARRPGASGSDAAPVDAPTVLIVEDNPDMRALL
                     HSLLERGYRVIEAVNGGEGLTAARERMPDLVITDVMMPVMNGYDLTRLIKEDAALAGT
                     PVLMLTAKADIAYKIEGLEYGADDYLTKPFNSRELLARVDSLLRTRAYQRIVEARNRE
                     IESELDIARLIIDRLLPQGAFDVSGYRFAASIIPMDTVGGDLYDCSIRGHFIDLFIAD
                     VSGHGLHAAFLSLITKMALESVTDRTLASRALYLVNDIIRRSTVK"
     gene            2495..3031
                     /locus_tag="PKJ16_08945"
     CDS             2495..3031
                     /locus_tag="PKJ16_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47156.1"
                     /translation="MIEYRVIDRQHPAPAWRPVFHSETKPDCVIGADKIGCILHNRLP
                     RQLLCISRIDEIVNGQHMGRIAEILGLPGDGNAINGIIEHDWRERMRGTDANLHYAND
                     EKKHAGYIFHYEGTANWQNMTHQWTHRQAEKGHRNGTRPDQTLLNPYSAFMHLQYGPE
                     SPAHNAAFVSVPSTGRLA"
     gene            3078..3854
                     /locus_tag="PKJ16_08950"
     CDS             3078..3854
                     /locus_tag="PKJ16_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015325096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HNV47157.1"
                     /translation="MTDRSHHISWNVNGIRAISKKGFADWLRAEGPDVLCLQETKAEL
                     EQFPKDVLAVEGYHLYASSAEKKGYSGTATFTKVEPEKVEYGIGEKRFDSEGRFVITH
                     YEKFILFNIYFPNGGASTERLQYKLDFYDAFLEYIDALVKKKKNVVFCGDVNTAHREI
                     DLARPKENEKNSGFMPIERAWIDRAVERGWVDTFRMFNDKPEQYTWWDYKTKARERNI
                     GWRIDYFFANKGFADAVMASTIMPDVEGSDHCPVALELKT"
     gene            4033..4491
                     /locus_tag="PKJ16_08955"
     CDS             4033..4491
                     /locus_tag="PKJ16_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47158.1"
                     /translation="MARTTLNLHINTMTEINRRSAECAVSRRSIIVRLLMMLMRDAHS
                     VDMRFTNVRYQPDDPSGNWHCFSILFREDEHEFFVDLRKIYKCSISLLVAIAVERYLD
                     EVAPIEPTKRHNYARFYNYLIQLDSVDGVMLWRIYWGFPRTEYLTPPSDH"
     gene            complement(4515..5153)
                     /locus_tag="PKJ16_08960"
     CDS             complement(4515..5153)
                     /locus_tag="PKJ16_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HNV47159.1"
                     /translation="MAEESNRRRAYEAAAQRAIAHLASIDLRDRCAHLGLPAPSSDGS
                     IAIRLLGREYALTSGDFRLVDASGVDAHPFDRVLALHYLCAEGRPQESGEYASFRELP
                     SGTFYWGPFRARSSAIIETVAGNDLELLRLGLAGFEVTPFPAGDVGAAVHAIGVVRIA
                     LVYRAGDDEFPPSAEVLFDSVVRRVFGTEDAAVLAERFAVRLVGEMRAHRSM"
     gene            complement(5157..5537)
                     /locus_tag="PKJ16_08965"
     CDS             complement(5157..5537)
                     /locus_tag="PKJ16_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47160.1"
                     /translation="MRKAVLYGGGVINILLAVFHMFFWSLLDWSTELPKLSVLNRGVL
                     QIANVIIIFVILYFAVMSFVMARHERLDVFGRSIIICITGFYAIRLVLGYPFFGFNYE
                     ELGVWIVCLIIIGAYLSVLRMEKR"
     gene            complement(5587..6927)
                     /locus_tag="PKJ16_08970"
     CDS             complement(5587..6927)
                     /locus_tag="PKJ16_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HNV47161.1"
                     /translation="MMMPQRTIIDSIVAGALAAVDPLRAVRRHLAFDGARILVDRPDG
                     GRRAYRMDDFERVLVVGCGKAAAPMAAAVEEILGGRIDEGVVVVKYGHGVDLARVGLV
                     EAGHPVPDENAVHGARTALDLLAAAGSRDLVIALISGGGSALLTLPAEGISLADKRKT
                     TELLLASGAAIGEMNTVRKRLSLVKGGLLARAAYPATVIALAVSDVVGDDIGVIASGP
                     FAPDESTFADALAVMHAYALGDAAPRTVIERLERGARGDIAETPRLGDPVFDRVMHRI
                     IASNALALEAARVAACACGYFAYVVPTPVIGDTADAARAHAAIARGIVERGAPVRRPA
                     CVISGGETTVAVRGGGRGGRNTEFAMHAAIAIDGMDGVCVASVGTDGTDGPTNAAGAC
                     VDGSTVARARALGMDSAAYVANNDSYSLFERLGDLIVTGPTRTNVMDVHIVIVD"
     gene            7300..8460
                     /locus_tag="PKJ16_08975"
     CDS             7300..8460
                     /locus_tag="PKJ16_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47162.1"
                     /translation="MKACQRRFTGSAVLALFAVILAGPIAAEATPATTAPRALPDREA
                     LRDCALFLSGRARPGGDQRRNAIIAQPFYTHVATSLARSWKAYASGHLEPMRRWMHRA
                     LPHTRRATVFYPFSGPDFPNAYAAYPEAETYLLIGLEPAGTLPSIEGMGDRNIARGML
                     FLHQLLETFTADNYFHTKEMALFNAWSPSIAGTSGLLLAFLGLMGFEPESIRPFVIDY
                     NGEERTLSRDVPEHRDALDKGEHSVEIAFTVPETGARKRIVYLSADLSNMRAIDRNPG
                     IFIYLERRMPEDVLIKAASYLLHLDNYRSMKRLIMRRARTIVMDDTGPRIAEFGPEWT
                     ITPYGEYRGTIELFANRTQPELTELYRARNPAKLPFAFGYGVGSRIMIITRR"
     gene            8587..9303
                     /locus_tag="PKJ16_08980"
     CDS             8587..9303
                     /locus_tag="PKJ16_08980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HNV47163.1"
                     /translation="MKSTVAFTGFPRETIRFYRELKKNNSKLWLEQHRDDYEAYVMAP
                     ARAFVREMGDRLASIAPGIIADPRVNHSIFKIHRDTRFSNDKTPLKEHLALWFWEGTR
                     PRMECSGFYFHLEPSGIMLGTGVYMFPKDMFPEYRDSVVHPKHGAVLAKAVKQVTGKG
                     YYVGGEYYKKVPRGYDPEHANAEYLRYGGFYAGIDEKPPAELASEKLLDFCLKRYRDM
                     LPIHRWLADMVERSVKNRAH"
     gene            complement(9430..11016)
                     /locus_tag="PKJ16_08985"
     CDS             complement(9430..11016)
                     /locus_tag="PKJ16_08985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV47164.1"
                     /translation="MMDPESRTMTRREFLGLAASTAAALALPTATGCMSVPLRDTYAL
                     NYSKDLVLANCSLIDVLSGAAVPDAMVRIREGKVVYAGRKTDAIGAPAEIIDLGGRYV
                     MPGLINSHVHMTLPCTSAFNPFFFPTFVRQMRRNFVLEIESGVTTVRDMASIPFVLDY
                     FVKDVENGNLRGPRVVHSTPFVNINGSHPDIPPFDISIFAPIAVAAVGNFGFNYEGWD
                     DLKKKIDRFVQKATFLKLSMDNRSILCGRGDVPVYTDEELKFLFDVAEKRHLRVSCHN
                     VMKFGFSRALKYPMHSVEHIVADDFITEAEARTMADRKIAIVPTAVVGNIYAYEEAFT
                     SLPAEYRDEEIDNELRIRREYWGGITDADCEPLIHRMNIESIAALRRLSFQQLIENKY
                     FVVNPHIIFKGLKFGKENLRRLDAGGVMMGVGTDAGTPMNYHGTIWRELEILSRAGIT
                     NDRILRSATAVNAKICGLDDRVGTLEPGKLADIVVLDGNPLVKIEACRKPALVFKGGK
                     LEVQRKDLRRNSEKGTVEVA"
     gene            complement(11091..12578)
                     /locus_tag="PKJ16_08990"
     CDS             complement(11091..12578)
                     /locus_tag="PKJ16_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNV47165.1"
                     /translation="MSELPMKFLRWGALCALLVCSVVPVRADIYDTNGFGDRGTSMSG
                     AVCASVRSLDALYYNVAGLGKVDASAMQFAYLGSYPRFDVDGGVSGVPENRDLETGMY
                     VTSMGVNLNTMARLPVTATFGFSVSIMDDLKVAVVEDLDESRHQFVRYGAPVKRTAVY
                     TGLGVEVVPNWVWIGVGGHGLVTGNAAVNLTIYPSDMSTTDAIKPSKQEFWLDSKLEF
                     SPSCGIIVNPWRTLNLGASYKKSIEVKFDPFDAVITMPIGSNNAAKVAAYTALLSFYN
                     PDVYRAGLSFELFGLTVEADAGFELWSHFHLSKPREGKRVVPKFKDIVTYHGGLEYAV
                     SIVRVRAGYAYVPSPVPDQPGASNFIDCDRHIVSAGLGLEFTDPLGIFYHPISLGISV
                     QDHIMARRNFSKDDGVDYSVTSHAYAAYASLTLRFSGSHTQRSKDDRARESLQEERRK
                     LETLLEENKKLLKELMEATEKRKKAEEGASNSKPNGAPETGNDKR"
     gene            complement(12596..>13316)
                     /locus_tag="PKJ16_08995"
     CDS             complement(12596..>13316)
                     /locus_tag="PKJ16_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47166.1"
                     /translation="LIDSVLEVASVLGDERDPEIISIRRSLTMGVARMFSDPATSANE
                     ENVREFSSWLLRKIDTAIADDWEYLDLAFDIGGRYVSPDYRIVDHVVGMLDPRYGVTA
                     QFGAILQHFHEKMTDPDSPMTQADVEGKLRWLVRLMELTSEKMLVSRTLVNAADIAER
                     LGETNPWHELAYVSMKAFDPEDGALSYVLMGMEKSHIYTWDEVIGDLIDFLRSDTMTR
                     NDEGSFWRDIYYISKFMADSL"
BASE COUNT         2779 a         4303 c         3883 g         2351 t
ORIGIN      
        1 tcttcaccgt ggagcggcgg atgatgtcgt tcacgagata aagcgcgcgg ctcgccaggg
       61 tgcggtcggt cacgctctcc agcgccatct tcgtgatgag cgacaggaag gccgcatgca
      121 gaccgtgccc ggacacgtcg gcgataaaga gatcgatgaa atggcccctg atggagcagt
      181 cgtagagatc gccgcccacc gtgtccatgg ggatgatgct ggcggcgaaa cgatagccgg
      241 aaacgtcgaa ggcgccttgc gggagcaggc gatcgatgat gagccgcgcg atatcgagct
      301 cgctttcgat ctcgcggttc cgcgcctcga cgatacgctg gtacgcgcgg gtccggagaa
      361 gcgaatccac ccgcgccagg agctcgcgcg agttgaacgg cttggtgaga tagtcgtccg
      421 cgccgtactc cagcccctcg atcttgtagg cgatatcggc cttggcggtg agcatgagta
      481 ccggcgtccc ggcaagcgcg gcatcctcct tgatcagcct cgtgaggtcg tagccgttca
      541 tcaccggcat catgacgtcc gtgatcacga gatccggcat ccgctcgcgc gcggccgtga
      601 ggccctcccc tccgttgacc gcctcgatga cccgataccc gcgctccaga agcgagtgca
      661 agagcgcgcg catgtcggga ttatcctcca cgatcaggac ggtcggcgcg tcgacgggcg
      721 ccgcgtcact gccgctcgcg ccgggccgcc gggcttccga accgtcatcc aatcgcgcaa
      781 ttgtggcgat gatgccggca ggcgcttcat cgccctcttc atcgccggcg aactcgacat
      841 ccgctcgacc cgcgagatgc cggcgaccca tcggaatccg caccgtgaag gtcgttccat
      901 ggtccccggc atgcgcgtca ccatgacggc tgtcaacggt gatcgccccg ccatgcagct
      961 cgacaaattc cttcgccagc gcgaggccga ttcccgaacc ctcgtagcga cgcgtcgaac
     1021 ccgaatcgac ctggctgaat cggtcgaaga tcgagccgag cttttcgaag gggatgccca
     1081 cgccggtgtc cgcgatctcg atggtgcaaa cggcgtcatc cgcactcacc cgcaccgtga
     1141 tcgccccggc ttcggtgaac ttgaaggcat tcgaaagaag gttcataagg atgcgctcga
     1201 acttctcggc gtcgagcata agcggtatgg gccgatcggg gagatcgagc gacaaggcga
     1261 gtccccgcgc ctccgcggcc gactgcacga caccgcagtg aaggcggacg agcgcggcgg
     1321 catcaacctc gcgcacgcga agcatcatgc ggcccgcttc gatcttcgag aaatcgagga
     1381 gattgttgat gagtcgcaac agacggacgc cgtttcgctg gatcgagcgg aagaaatcct
     1441 cgcccaccgg cttcccgtaa tcaccctgca gcaccgattc taccggcgag aggataagcg
     1501 tgagcggcgt gcggatctcg tgcgaaacgt tcgcgaagaa gttcgtcttt atgcggtcca
     1561 tctcggtgag cttctcgttc gccagagaaa gctcccgggt gcgctcgtcg acgcgattct
     1621 ccagatcgac gttgaggacc tgaagctcgt tcttcattac attgatgcgg tccgcgaggc
     1681 caagcgagaa gatgaccgcc tgaatggcgt tgctgatttc ccacccgtaa tacgtgacga
     1741 tgccgtgagg caccagattg aacgccacaa ccgacgacag cagaacgccg attatcatca
     1801 gggtttgagc gccgatataa taccatgcct cccgtcgtcc cttcatggcg cacatgacgc
     1861 caatagccat gaaaaagcat gcccccggca tatgaacaag cataacagca ttgagcatga
     1921 tatccggcgc aacgatcgac agggcggcca ggacgacata gagcgccgcc acaatgcgga
     1981 gtgcgaaatc gattcgcggg aattgcacat ccattttaag gtatgagcgc gcaaagagcg
     2041 cgaaaaaaac cgtagtatac gccatcgcga agggaagcgt tctgccgagc cacgcgccct
     2101 cgggaaacag gtactgcatg cccagacccg tgaatgacac tgaaagaatc agatagccga
     2161 gcataaacaa aacataatat agataattga catcccgcag cgagatgaag acgaatagat
     2221 tataggcgac catacccagg agcagcccca tatacatgct ctggaaaata agttcgatgc
     2281 ggtccaaccg cgcgtgcatt gcagcgggta gaacgcgcat gggcacgata aacgcctgct
     2341 tcgtagccac gcgcatatag tacgcggcgg gcgcaccgtg cctccccaat gggaacacgt
     2401 gattgacgct atcgacgacg cgccgagtga acggaatgag atcacccgac tccatcatgg
     2461 aatacccgtt cacgcccggg gagaagaatt gcacatgatc gagtatcgtg taatcgaccg
     2521 ccagcaccca gcccctgcct ggcgacctgt tttccacagc gaaacgaaac cagattgcgt
     2581 cattggagct gataaaattg ggtgcatcct gcacaaccgg ctgccacgcc agctgttgtg
     2641 catttcgagg atcgatgaga tcgtcaatgg tcagcatatg ggacggatcg cggagatact
     2701 cggccttccg ggcgatggca atgcaatcaa cggaatcatc gagcacgatt ggcgtgagcg
     2761 catgcgcggg accgacgcca atttgcacta cgcaaatgac gagaagaaac atgcaggcta
     2821 tatatttcat tatgagggga ccgccaactg gcagaacatg acacatcaat ggacccaccg
     2881 tcaagcagaa aaagggcatc ggaacgggac ccggcctgat caaactctac tcaaccccta
     2941 ttcagccttt atgcatctcc aatacggccc tgaatcgcca gctcataatg ccgcattcgt
     3001 ctccgtgcca tccaccgggc gattggcgta aaacaatcat tgacaataca agatccgcac
     3061 cgaaaaatac gcacacattg acagatcgga gccaccacat cagctggaac gtgaacggca
     3121 ttcgcgccat atcgaagaag ggcttcgcgg actggttacg cgcggagggc cccgacgtcc
     3181 tttgcctgca ggagacgaag gccgagttgg agcagttccc gaaggatgtc ctggcggtcg
     3241 aggggtatca tctgtacgcg tcgtcggcgg agaagaaggg atacagcggg acagccacct
     3301 tcacgaaagt ggagccggag aaagtcgagt acggaatcgg cgagaaacgc ttcgacagtg
     3361 agggacgttt cgtcatcacc cattatgaga aatttattct tttcaatata tatttcccga
     3421 acggcggcgc atccacggag cgcctgcagt acaaactcga cttctacgac gccttcctcg
     3481 aatacatcga cgcgctcgtc aaaaagaaga agaatgtggt tttctgcggc gacgtgaaca
     3541 cggcgcaccg ggagatcgac ctcgcgcgcc cgaaggaaaa cgagaagaac tcgggcttca
     3601 tgcccatcga gcgcgcgtgg atcgatcggg ccgtcgagcg cggctgggtg gacaccttcc
     3661 gcatgttcaa cgacaagccc gagcagtaca cttggtggga ctacaagaca aaggcgcgcg
     3721 agcggaacat tggctggcgg atcgactact tcttcgcgaa caagggattt gcggacgcgg
     3781 tgatggcgtc gaccatcatg cccgatgtgg agggctcgga ccactgcccc gtagcgctcg
     3841 agctgaagac gtgactccac tgtcgggtgc ccgtttcaga tatcagaacg catccatccc
     3901 tcctccagta cgagccgagg agccccatca aaagtgaaca ccgcactgga gtaaattaat
     3961 aatcattcca ataaccacgc gccgaacccg tattccctat gacaacacac cggcagagaa
     4021 cgggaggcga aaatggcgcg gaccacactc aatcttcaca tcaacaccat gacggaaatc
     4081 aatcggaggt cggctgaatg cgccgtatcg cgccggtcca tcatcgtgcg tctgctcatg
     4141 atgctcatgc gggacgccca ttcggtggac atgcgtttta caaacgtccg ttatcaaccg
     4201 gacgatccat cgggaaactg gcattgcttt tcgattctat ttcgggagga tgagcatgag
     4261 ttttttgtcg atttgcggaa gatttacaaa tgctcgattt ccctgttggt ggcaattgcc
     4321 gttgagcgat atcttgacga agttgcaccc attgagccta caaaaaggca taactatgca
     4381 cgattctaca attacctcat tcagctagat agcgtagacg gagtaatgct ctggcgaata
     4441 tactgggggt ttccccgaac ggaataccta acgcccccca gcgaccacta acgcccgcat
     4501 ccatccgagg cccatcacat cgatcgatgc gcacgcatct caccgaccag ccgcacggca
     4561 aagcgctcgg cgaggacggc cgcatcctcg gtcccgaaga cgcgacgcac gaccgaatcg
     4621 aacaggacct ccgcggaggg gggaaactca tcgtcgcccg cgcgatagac gagcgcgatg
     4681 cgaacaacgc cgatcgcatg aaccgccgcg cccacatcgc ccgcgggaaa cggtgtcact
     4741 tcaaaaccgg caagaccgag ccgcagaagc tccagatcgt ttcccgcgac cgtttcgatg
     4801 atggccgacg agcgcgcccg aaacgggccc cagtaaaaag ttccggacgg gagctcccgg
     4861 aacgatgcat actcgccgct ttcctgcggc ctcccctcgg cgcacagata atgcaatgca
     4921 agcacgcgat caaagggatg cgcgtcgaca ccggacgcgt ccacgagccg aaagtcaccg
     4981 ctggtgaggg catactcccg cccgagcaac cggatcgcga tcgagccgtc gctcgaaggc
     5041 gccggcagac ccaggtgcgc gcatcgatcg cgcaggtcaa tcgacgccaa gtgcgctatc
     5101 gcccgctgcg ccgccgcctc gtatgcccgc cgcctgttgg actcctcggc catgcgttac
     5161 cgcttctcca tccgcaacac ggacagatac gcgccaatga taatgaggca cacaatccac
     5221 accccaagct cctcgtaatt aaacccgaaa aacggatatc cgagcaccag gcggatcgcg
     5281 taaaacccgg tgatgcatat aatgattgag cgaccgaaaa cgtccagccg ttcatgccgc
     5341 gccatcacga acgacatcac ggcgaagtac aggataacga aaatgatgat gacattcgcg
     5401 atctgcaaaa cgccgcgatt cagcaccgag agtttcggca gctccgtcga ccagtccagg
     5461 agcgaccaga agaacatgtg gaacaccgcc agcagaatgt tgatgacgcc gccgccgtac
     5521 aataccgcct ttctcatgca aatacctcca gatggaaaac aatattcgcc gcacccgcga
     5581 cgcatctcaa tccacgatga cgatgtgcac atccatcaca ttcgtccgcg tgggtccggt
     5641 cacaatgagg tccccgagcc gctcaaagag agagtacgaa tcgttgttcg cgacatacgc
     5701 ggcactatcc atgcccaatg cccgcgcccg tgcaacggtg gaaccgtcga cacaggcgcc
     5761 cgcggcattc gtcggtccgt cggtgccgtc cgtgcctacg ctcgcgacgc acaccccgtc
     5821 cataccgtcg atcgcaatcg ccgcgtgcat cgcgaactcc gtgttgcgac cgccacgccc
     5881 gccaccgcgc accgcgaccg tcgtctcgcc gccgctaatc acgcaggccg gccgccggac
     5941 gggcgcgcct ctctcaacga tcccccgcgc aatcgccgca tgggcccggg cggcgtcggc
     6001 ggtatcgcca atcacgggcg tcggtacgac atacgcgaaa tatccgcagg cgcaagcggc
     6061 gacgcgggcg gcctctagcg ccaacgcatt cgaagcgatg atccgatgca taacccgatc
     6121 gaacacggga tcacccagcc gtggcgtctc cgcgatatcg ccccgagccc cccgctcgag
     6181 ccgctcgatc accgtgcgcg gcgcggcgtc gcccaatgca taggcatgca tcaccgcgag
     6241 ggcgtcggca aaggtcgatt cgtccggtgc gaacggtccg gacgcgatca cgccgatatc
     6301 atcgcccaca acgtccgaaa ccgcgagcgc gatcaccgtc gccggatacg cggcccgcgc
     6361 gagcagccca cccttcacga gcgagaggcg ctttcgcacc gtgttcatct cgccgatggc
     6421 ggccccgctg gcgaggagaa gctccgtcgt cttccgcttg tccgcaaggg atatcccctc
     6481 cgccggaagc gtcaaaagcg cggagccgcc gccggagatg agcgcaatca ccaggtcgcg
     6541 ggaaccggcg gccgcgagga ggtccagcgc cgttcgcgca ccgtgcaccg cgttctcgtc
     6601 ggggaccggg tgccccgcct cgacgagacc cacccgcgcg agatcgacgc catgcccgta
     6661 tttcacgacg acgacgccct cgtcgatccg gccgccgagg atctcctcga cggccgcggc
     6721 catcggcgcg gctgccttgc cgcaaccgac gaccagcacc cgctcgaaat cgtccatgcg
     6781 atacgcccgc cggccgccat ccggccggtc gacgagaatg cgcgcgccat caaaggccag
     6841 gtgacggcgc acggcccgca ggggatcgac cgctgcgagc gcgccggcca caatcgagtc
     6901 gattattgtc cgctgcggca tcatcatccc ccacgcctcc cgacagacgc accgtggggc
     6961 gattcccgga ggagcgcaat tcattttcgc cgaatgacct ccgcgcgcaa ttcgtgcttg
     7021 acacgcgggc gtatccgcca tcgtcttcaa gcatgcccaa ccggctcgag cactcttcac
     7081 cactggagaa cacccatgaa caaatgcgga accgagatga tgagcatgat gattttcttc
     7141 gccgttctcg tggtcggatt cgtgacggcg cgctatcgcc ggtagcgtca cgggaaatat
     7201 gcgaccgcgc ccggaacggc gctcaccgcg cacacgtcgt gaagcggcgt atcgtcatac
     7261 cgtcgtcgta tcgtattcta tttcgaagcg agcgtatcca tgaaagcatg tcagcgccga
     7321 ttcaccggga gcgccgtact cgctctcttt gccgtcatcc ttgccggccc catagccgcg
     7381 gaagcgacac cagcaacgac cgcaccgaga gcgcttcccg atcgtgaggc gctgcgtgac
     7441 tgcgcgctct tcctctcggg ccgcgcgcgt ccgggcggcg accagcggcg aaacgccatc
     7501 atcgcacagc cgttctacac gcacgtcgcg acgagccttg cgcgctcatg gaaggcatac
     7561 gcgagcgggc atctcgagcc gatgcgccgc tggatgcatc gcgccctgcc gcacacgcgt
     7621 cgcgcgacgg tattctatcc tttttccggt cccgacttcc cgaacgccta cgcggcctac
     7681 cccgaggccg agacctatct gctcatcggc ctcgagccgg ccggtacgct tccatcgatc
     7741 gagggcatgg gcgatcggaa catcgcccgg ggcatgctct ttctccacca gctcctggaa
     7801 accttcacag ccgacaatta tttccacacc aaggagatgg cgctttttaa cgcgtggagc
     7861 ccctccatcg cggggacctc gggcctcctg ctcgccttcc ttggcctcat ggggttcgag
     7921 ccggagtcca tacgcccgtt cgtcatcgac tacaacggcg aggagcgtac gctctcccgg
     7981 gacgtgccgg agcaccgtga cgcgctggac aagggcgagc actcggtgga gatcgcgttc
     8041 accgttcccg aaacgggcgc gcgcaagcgg atcgtctatc tctcggcgga cctgtcgaac
     8101 atgcgcgcca tcgaccgcaa tcccggcatt ttcatatatc tcgagcgccg catgccggag
     8161 gacgtgctca tcaaagccgc ctcgtacctg ctgcacctcg acaactaccg ctcgatgaag
     8221 cggctgatca tgcgccgggc gcgcaccatc gtgatggacg acaccggacc gcgcatcgcg
     8281 gagttcggac ctgagtggac catcaccccc tacggggaat accgcggcac catcgagctc
     8341 ttcgcgaacc ggacgcagcc ggagctcacc gagctctatc gcgcgcgaaa tccggcgaag
     8401 ctgccgtttg ccttcgggta cggagtgggg tcgcggatta tgatcatcac ccgtcggtag
     8461 acaaaccata cgtgagcttg ctacgatgag aatcatcgac accgtcatcg caccatcgca
     8521 acgcttcgtc cttcaggaac atgcggccga tcccgctccg cggcgttgcg cacaaggaca
     8581 ctggacatga aatcaaccgt cgccttcacc ggttttccca gggaaaccat ccgcttttac
     8641 cgggagctca agaagaacaa cagcaagctc tggctcgagc agcaccgcga cgactacgag
     8701 gcatacgtca tggcgccggc gcgcgccttc gtgcgcgaga tgggcgaccg gctcgcgtcg
     8761 atcgcgccgg gcatcatcgc cgatccccgc gtgaaccact ccatcttcaa gatccaccgc
     8821 gacacgcgct tctcgaacga caagacgccg ctcaaggagc acctggcgct ctggttttgg
     8881 gaggggacgc ggccgcggat ggagtgctcg ggattctact ttcacctcga gcccagcggc
     8941 atcatgctgg gaacgggcgt gtacatgttc ccgaaggata tgttcccgga ataccgggac
     9001 tcggtggtgc acccgaagca cggggcggtg ctggcgaaag cggtcaaaca ggtgacggga
     9061 aaaggctact atgtcggggg cgaatactac aagaaggtcc cgcgcggata cgacccggaa
     9121 catgcgaacg cggaatacct ccgctacggc ggattctacg ccggcatcga cgagaagccg
     9181 cccgcggagc tcgcatcgga aaagcttttg gacttctgcc tgaagcggta tcgcgacatg
     9241 ctgccgatcc atcgctggct ggctgacatg gtggaacgct cagtgaaaaa tcgggcgcac
     9301 taaccgaagg ggccgatccc gctccgcggc gccgctgata cagacgggac gccgcgacag
     9361 tcatcgttca atcatcacaa ccccggcagc gcgatcgccc gcgcagagga gagcgcgccg
     9421 gaacacacgt tatgcgacct cgaccgtccc cttctcggaa ttccggcgca ggtccttccg
     9481 ttgcacctcg agcttcccgc ccttgaacac cagcgcgggc ttccggcagg cctcgatctt
     9541 caccagcgga ttcccgtcga gaacgacgat atcggccagc ttgcccggct cgagcgttcc
     9601 cacccggtca tcgaggccgc agattttcgc gtttaccgcg gtggcgctcc tcaggatgcg
     9661 atcgttcgtg atgccggcgc gcgagagtat ctccagctcg cgccagatgg tgccgtggta
     9721 gttcatcggg gtgccggcgt cagttcccac gcccatcatc accccgcccg cgtccagacg
     9781 gcgcaggttc tccttcccga acttgagccc cttgaagatg atgtgggggt tcacgacaaa
     9841 atacttgttc tcgatcagct gctggaagct gagccggcgc aacgccgcga tcgactcgat
     9901 gttcatccgg tggatcagcg gctcgcagtc ggcgtcggtg atgccgcccc agtattcgcg
     9961 gcggatccgc agctcgttgt cgatctcctc gtcccgatac tccgccggga gcgacgtgaa
    10021 ggcctcctcg taggcgtaga tgttccccac cacggccgtg gggacgatgg cgatcttccg
    10081 gtccgccatc gtccgcgcct cggcctccgt gatgaaatcg tcggccacga tgtgctcaac
    10141 cgaatgcatc gggtacttca gcgcgcggga aaatccgaac ttcatcacgt tatggcacga
    10201 cacgcgcaga tggcgcttct cggccacgtc gaacaggaac ttcaactcct cgtcggtata
    10261 caccgggaca tcgcccctgc cgcataaaat ggagcggttg tccatggaga gcttcagaaa
    10321 ggtcgccttc tgcacgaaac gatcgatctt ctttttcagg tcgtcccagc cctcgtagtt
    10381 gaagccgaaa tttcccacgg ccgccacggc gatcggcgcg aagatcgaga tgtcgaacgg
    10441 cgggatgtcc ggatggctcc cgttgatgtt cacgaaaggc gtgctgtgca ctacgcgtgg
    10501 accgcggaga tttccgttct cgacgtcctt cacgaagtaa tccagcacga agggaatgga
    10561 ggccatatcg cgcacggtgg tcacgcccga ctcgatctcg aggacgaagt tgcgacgcat
    10621 ctggcgcacg aaggtgggaa agaagaaggg attgaaggcc gaggtgcagg gcagcgtcat
    10681 gtggacatgg ctgttgatga gccccggcat cacgtatcgc ccgcccaggt caatgatctc
    10741 ggccggggcg ccgatggcgt ccgtttttct ccccgcgtat accaccttcc cctcgcggat
    10801 gcgcaccatc gcgtcgggaa ccgcagcccc cgacagcacg tcgatgagcg agcagttcgc
    10861 gaggacgagg tccttcgaat aattgagcgc gtaggtgtcc cgaaggggca ccgacatgca
    10921 gccggtcgcc gtggggagcg caagcgccgc cgcggtcgac gccgcgaggc ccaggaactc
    10981 gcgacgcgtc atcgttcttg attcagggtc catcatgcca tacacctctt ggatacagtt
    11041 ccatcattcc gtttcttggt atctcgacaa catcgagtca agcgacatat ctatcgttta
    11101 tcgtttcccg tctccggcgc gccgttcggt ttcgaattcg acgcgccctc ctccgccttt
    11161 tttcgtttct ccgtcgcctc catgagctcc ttgagcagct tcttgttctc ctccagcagc
    11221 gtctccagct tgcgtcgctc ctcctgcagc gattcgcgcg cccggtcgtc cttcgagcgc
    11281 tgcgtgtgcg aacccgaaaa gcgcagggtg agcgacgcat aggcggcata ggcgtggctg
    11341 gtaacggaat aatcgacgcc atcgtccttt gaaaaattcc gtcgcgccat gatgtggtcc
    11401 tggaccgata tcccgagcga tatggggtgg tagaaaatcc ccagcgggtc ggtgaactcc
    11461 aatcccaggc ccgcgctcac gatatgccgg tcgcagtcga tgaagttgga cgcccccggc
    11521 tggtccggaa cgggcgacgg gacgtatgcg tagccggcgc gcacgcgaac gatggacacc
    11581 gcgtattcga gcccgccgtg ataggtcacg atgtccttga acttgggcac cacgcgcttc
    11641 ccctcgcggg gcttgctcag atgaaaatgc gaccacagct cgaagcccgc gtcggcctcc
    11701 accgtgaggc cgaacagctc aaaggacagt cccgcccggt agacgtccgg attgtaaaaa
    11761 gaaagcagcg cggtgtaggc ggcgaccttc gcggcgttgt tcgagccgat gggcatggtg
    11821 atgacggcgt cgaagggatc gaacttcacc tcgatgctct tcttatacga cgcgcccagg
    11881 ttcagcgtgc gccagggatt cacgatgatg ccgcaggacg ggctgaactc gagcttcgaa
    11941 tcgagccaaa actcctgctt ggacggcttg atcgcgtcgg tggtggacat atcggacggg
    12001 tagatggtaa gattgaccgc cgcattgccg gtgaccagcc cgtgcccgcc gacgccgatc
    12061 cacacccagt tcggcaccac ctcgacgccc aggccggtgt acaccgccgt gcgcttgacg
    12121 ggcgcgccgt agcggacgaa ctggtgccgc gactcgtcga ggtcctccac caccgccacc
    12181 ttcaggtcgt ccatgatcga gacgctgaag ccgaaggtgg ccgtgacggg gagtcgcgcc
    12241 atggtgttca ggttcacccc catgctggtc acgtacatgc ccgtttcgag gtcccggttc
    12301 tcggggaccc cgctcacgcc gccgtccacg tcgaagcgcg ggtacgaccc caggtaggcg
    12361 aattgcatgg cgctcgcatc caccttgccc agcccggcca cattgtagta gagcgcgtcg
    12421 agcgaacgaa cgcttgcgca caccgcgccg ctcatcgacg tgccgcgatc accgaacccg
    12481 ttggtgtcgt agatgtcggc gcgcacggga acgacactgc agacgagcag cgcgcacagc
    12541 gctccccatc gcaggaactt catcggtaac tcggacaccg tcccctcctg tcgcatcaca
    12601 gcgaatcggc catgaacttc gagatgtaat agatgtcccg ccagaacgac ccctcgtcgt
    12661 tacgcgtcat cgtatcggac cgcaggaagt cgatgaggtc gccgattacc tcgtcccagg
    12721 tgtagatgtg gctcttctcc atgcccatga gcacgtagct cagcgcgccg tcctcgggat
    12781 cgaacgcctt catggagaca taggcgagct cgtgccaggg gttcgtctcg cccaggcgct
    12841 cagcgatgtc cgcggcgttc acgagggtgc ggctcacgag catcttctcg ctggtcagct
    12901 ccatcaggcg caccagccat cggagcttgc cctcgacgtc ggcctgcgtc atggggctgt
    12961 cgggatcggt catcttctcg tggaagtgct gcaggatcgc gccgaactgc gccgtcacgc
    13021 catagcgcgg gtccagcatc cccaccacat ggtcgacgat ccggtagtcc ggcgatacat
    13081 atctcccgcc gatgtcgaag gccaggtcca gatactccca gtcgtccgcg atggcggtgt
    13141 cgattttacg gagcagccat gacgaaaact cgcggacgtt ctcctcgttc gcgctggtgg
    13201 ccggatcgga gaacatgcgc gcgaccccca tggtcaggga gcggcgaatg ctgatgatct
    13261 ccggatcgcg ctcgtcgccc agcacgctcg cgacctcgag gacgctgtcg atgagc
//
LOCUS       DAORHF010000088        13306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3713_length_13306_cov_4.632103, whole genome shotgun sequence.
ACCESSION   DAORHF010000088 DAORHF010000000
VERSION     DAORHF010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13306
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3713_length_13306_cov_4.632103"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PKJ16_09000"
     CDS             <1..724
                     /locus_tag="PKJ16_09000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV47167.1"
                     /translation="RYTVARGDTLWSILAKSGLNIDTLVAINGLSSPSQVRPGVTLFI
                     PNMRGIIIDTHNRDTLDGVAAHHRIAPVYLAHINRCAQGVRRYLFVPNAQLSSLERSL
                     FLGTGFYHPFKNERLTKGRISSGFGNRIDPIQKSIKFHSGVDIACPVGTRVYAARSGE
                     VVFAGTRGGYGQLIIIKHPHDYYSYYGHLQRIMVRTGDRVTTRQQIALSGNTGRTTGP
                     HLHFEIRKNTKPVNPDVLARLR"
     gene            complement(730..2811)
                     /locus_tag="PKJ16_09005"
     CDS             complement(730..2811)
                     /locus_tag="PKJ16_09005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV47168.1"
                     /translation="MGLLEKALKYKKEINRKGRETLIDTIPGPAETDFVDEAAMEDSG
                     DRSAAAAHDAVTDQPATGADADDAVIRDDELALLDAEDLKQVDEPDDLFTLPDDDGAA
                     PHEVIESQRQGTPRATAVDAGSAPLAGAVRRSGSAAHAAPQFTGPFGTTDEPDLKGAP
                     AVQRSTEQQVVIRDEFSTARAIEATADSVDGAPGEAVDEPSDRAGANGKPDKRAQDFM
                     ILFEIGKEIMSAENRKALYDAILFSIMGQIGASSSSIMVRSSEDTARWTIADSRGVTI
                     RNKQLYFDESGAIFKSLLESGGIIDIETYKDDPEHRDEYYKFISIDGRLLAPLSHAGE
                     IVGILVLGDKITIGNYSDEECDFIAAMGEISAVAMRKINMIDRLQEERDELHAASEHV
                     ANVDTLARRMIEFSSLRGLCDIVARELGSMSVESFAVFIGDERNDVFVPVMTEDNDLI
                     GLRGDDVRIAFDSPFTDLVQDLKDRAQIADFDRLKIIRDAFGESAIKRMTHFWVYPFK
                     IGVKLLGFVLVFQIADVEREREIDRMIGRLSTFLVPYLLMTMRMDFEENRYVDNIELV
                     LKRINREILKAQSLKIPLTLVLFSIKNFKRYLHLFGLVEAKKLIAVLEGIIVERLSDP
                     DFAIRYDRNKILIVLPGKNKKFSVPLANTIRNEVLQRFKNREMQLLVTFLTAEYPEDG
                     ADLYTLLDGID"
     gene            complement(2813..5320)
                     /locus_tag="PKJ16_09010"
     CDS             complement(2813..5320)
                     /locus_tag="PKJ16_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47169.1"
                     /translation="MDTRHTKTRDIQFEEITDISGDLHEAEYIDDDAAAPAAGSPHDH
                     DVMKDHGAGSWEGVLEEQTRITEGRAEPRVIDDAYQPNSFDIEALSADVDRRPRRREQ
                     KETVEASVNYTTLSSDIDMDLVVDGEANDDTPRIAPERIVADDAAFSAPVRDVGPATP
                     PVTEDEFARLEIDMNEIETVLSESDLSTADSIALEHAAVARTPGADRGEEAVTRAGGD
                     LELIDAPSEDAEPSIDQARIEHFSDAELESFLDDAPPSGGRIGDELEFDFPDDDVLAR
                     SGADRRIGDKDRREVDDGFIETGGQSGKKNRITIELDDGVADRLPDDFDITELGAIDL
                     REAEAVANEDVVFLTEEDLIEELQDVDLVFLAENGAVEDSQEDDALRAHSARDRRRKD
                     ERPDELIEVIDDDAHGDVASEVDGGEGAVEELMLDAEGREMEPAAPQTDSGVARGSKR
                     REDDRGSTHADDAVFSGTDGALAELETYDGNDFDVVFDTLDKSRQPDSGTGALLQSRD
                     SKPAVTPRAAVRRERESLPHDIIAIAEKFPGTQVIDDGLVERGDEAAPAEIFRQDELD
                     RITADIIETVESDARLLEEANVADDQGKIASVMKGSAPAFEDLLIDFEDEYQFVDNEL
                     KFIDAAILDDVQKNGFDGTDNAPARRAKKTTQAVEIVGLTADEIGVIEKSVFAGEYDA
                     VVLPAAPKAGMAGGDRVAARSESADEMQYRLPSPESLNADERASIEEDLASGAAYVFE
                     ENVADIRRKLDHLVREKEAGAAGAPDIGADVVFIDNKSDVDRFVETLPAAKREDMRKL
                     LAYLDGLFEKLPEEVVQRFAESEYFSLYQDVLKEMGL"
     gene            complement(5581..5967)
                     /gene="atpC"
                     /locus_tag="PKJ16_09015"
     CDS             complement(5581..5967)
                     /gene="atpC"
                     /locus_tag="PKJ16_09015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HNV47170.1"
                     /translation="MTMKLNCSILTPERTLYEGQVEFAIVQAHDGEIGFLYNHAPLVS
                     ELGVGEVRLRTANTTDFFYVEGGFVEIKENDLILICEQAMRKDELVRSEIEYKINDFT
                     KLDVKERMKLDIELRKLKARLKVAAR"
     gene            complement(5972..>6254)
                     /locus_tag="PKJ16_09020"
     CDS             complement(5972..>6254)
                     /locus_tag="PKJ16_09020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593782.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNV47171.1"
                     /translation="QDIIAILGMDELSEEDKLLVQRARKIERFLSQPFFVAEQFTGMP
                     GKYVKLEETIRSFNGLIKGEYDSLPEQAFYMVGTIEEVVEKAKKLQAGS"
     assembly_gap    6255..6264
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6265..7399)
                     /gene="atpD"
                     /locus_tag="PKJ16_09025"
     CDS             complement(<6265..7399)
                     /gene="atpD"
                     /locus_tag="PKJ16_09025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HNV47172.1"
                     /translation="MAQNVGKVVQVIGSTLDAEFPEGHLPELYNALTLEIDVMGAKSL
                     ITCEVQQHLGGSRVRAVALASTDGIRRGTPITDTGAPISVPVGEETLGRIFNLLGQPV
                     DNGPAVNTKDRRSIHQHPPKFEQLEPKAEIFETGIKVIDLLAPYIKGGKTGLFGGAGV
                     GKTVVIMELINNVAQQHGGYSVFAGVGERTREGNDLWLEMKESGVINKACLVYGQMNE
                     PPGARLRVALSALTMCEYFRDMSGRDVLLFIDNIFRFSQAGSEVSALLGRMPSAVGYQ
                     PTLSTEMGELQERITSTKYGSITSVQAIYVPADDLTDPAPATAFIHLDASTVLSRQIA
                     EKGIYPAVDPLDSSSRLLAPELVGQEHYDVAMEVKRILQRYKDL"
     gene            complement(7511..8395)
                     /gene="atpG"
                     /locus_tag="PKJ16_09030"
     CDS             complement(7511..8395)
                     /gene="atpG"
                     /locus_tag="PKJ16_09030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HNV47173.1"
                     /translation="MATQREIKKRIESVKSTKKITKTMEMVSTAKMKKMQGRLLMSQP
                     FVQKLNQVIANLRNSGVENIYDPLMQEWPESKRTLVFTVTGNRGLCGGFNNNVINNTI
                     AFKKRLEIEEGRDVQIYAIGKKAIGYFNFTGQPMYKSSLNMEDKVSFAEAAKIAQELI
                     NLYEYGEAHEVYLSYTKVVSASSQKPVIVQLLPIKPERVGVEDVGPEFTPQYIFEPNP
                     YRIFSSLLPLYVKAQVFLALLESNFSEQFARRVSMKNATDAATDMIRDLTITYNRARQ
                     AKITTEIAEIVGGAAALG"
     gene            complement(8439..9953)
                     /gene="atpA"
                     /locus_tag="PKJ16_09035"
     CDS             complement(8439..9953)
                     /gene="atpA"
                     /locus_tag="PKJ16_09035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HNV47174.1"
                     /translation="MKIKTEEIKSIIRKEIEGYKKEFNVSEVGTVIQVGDGIARLYGL
                     SNAMAGEMLEFQNGAYGLVFNLEEDSIGAVVLGDYLTIEEGHTVKRLDRVLAVPCGDA
                     LLGRVVNPLGIPIDNKGPINTTALRPMEFLAPGIASRQPVKEPLQTGIKSIDSMTPVG
                     RGQRELIIGDRKTGKTAIAIDTIINQKGKNVICVYVAVGQKDSTVAAVVEKLEQHGAM
                     EYTIVVVAGAAEAAPLQYIAPYAGAAMAEYYMYEKGGHTLCVYDDLTKQASAYRQLSL
                     LLRRPPGREAYPGDIFYCHSRLLERAAKLSDEMGGGSMTALPIIETQEGEVSAYIPTN
                     VISITDGQIYLVPNLFASGIRPAVDVGISVSRVGGNAQIKAMKKYAGSLRLDLAQYRE
                     LEAFAQMGTELDAATQQQLDRGARLIEILKQGQYVPMSVAEQVAVIFAGTQGYIDKVP
                     VEKVKDFEVKFIKYLNEQQADILDEISKTGELKSIDRYKKVCSDYAEGYLAGRA"
     gene            complement(9943..10500)
                     /gene="atpH"
                     /locus_tag="PKJ16_09040"
     CDS             complement(9943..10500)
                     /gene="atpH"
                     /locus_tag="PKJ16_09040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HNV47175.1"
                     /translation="MVANVYANALVEIGQEKKILDRLEEELGFIVGLISEDRDVEIYL
                     NAPGISPDAKKEFIDRVFGGEFSETIVNLLKVLVDNGRQALLKSINEAFVELLDVVNN
                     RQRVTVISSMKLGKDVLDKVQASLSEKLKKNIILDDAVDESILGGIILRMGDIILDGS
                     MANDLKNIRHNLLNSKVRGDRAYED"
     gene            complement(10567..11076)
                     /gene="atpF"
                     /locus_tag="PKJ16_09045"
     CDS             complement(10567..11076)
                     /gene="atpF"
                     /locus_tag="PKJ16_09045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HNV47176.1"
                     /translation="MDAIREGLLKVDPGLYLWTLVTFIVLVLILWKAAWKPLVEALDL
                     RAEKVRNDIETAEKNRIEAEKIFEDHKAMMNNAKNEAAKLIADGKADAERLRSGLLEK
                     ANEEAQKIMEHAKREITLSKDKALGELKNEIALLSTEIASKIIVKNLKPEDQKAIVEE
                     ALTKIRTVQ"
     gene            complement(11199..11423)
                     /gene="atpE"
                     /locus_tag="PKJ16_09050"
                     /pseudo
     CDS             complement(11199..11423)
                     /gene="atpE"
                     /locus_tag="PKJ16_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935489.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
     gene            complement(11592..12476)
                     /gene="atpB"
                     /locus_tag="PKJ16_09055"
     CDS             complement(11592..12476)
                     /gene="atpB"
                     /locus_tag="PKJ16_09055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HNV47177.1"
                     /translation="MLKLISLVNMVVGAGGGHGGKEESVSDVVMHHLTDHVVEGNFIA
                     KILEPVNTVLGGITFEVLGLKSNLAITKLVLMMWLAAFICIIIFIPVARKIKNAKYGS
                     SSKWVNMWEVLVGFVHDEIVEPNFGHHYKKIAMPWFCTTFFFVLFCNLLGMIPYMSTA
                     TGNLAVTGALASTTLLGMIVIGTIKQGPQWIFTGIVPGGVPLPLFPLLWVIELMGLLI
                     KPFALTVRLYANMTAGHIVIIIFIFLVMMFKSALVGIGSVAGALMINMLELLVAFIQA
                     YIFTALSAMFIGASLHAH"
     gene            complement(12528..12767)
                     /locus_tag="PKJ16_09060"
     CDS             complement(12528..12767)
                     /locus_tag="PKJ16_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HNV47178.1"
                     /translation="MAYSTIGLQLAITILLFIYVGYKADEHFRSSPWLFILSVVLGMT
                     AGFYNLIRELRNIDRFLENKPAADSQDGEKRTKWL"
     gene            13041..>13306
                     /locus_tag="PKJ16_09065"
     CDS             13041..>13306
                     /locus_tag="PKJ16_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47179.1"
                     /translation="MNRTKKHAAGRERPDDDHAGSTMKLSIKLIECDREYIVSCPELD
                     INCYAPTKNEALRRIVTVISFYIDSAKELGLEVGGIEAINVEGLP"
BASE COUNT         2721 a         4074 c         3576 g         2925 t
ORIGIN      
        1 ccggtacacc gtcgcccgcg gcgacaccct gtggagcatc ctcgcgaaaa gcgggctcaa
       61 catcgacacc ctcgtggcca ttaacggcct ttcgtcgcca tcgcaggtgc gtccgggcgt
      121 gacgctattc atccccaata tgcgcggcat catcatcgac acgcacaacc gagacactct
      181 cgatggggtc gctgcgcatc atcgcatcgc cccggtgtat ctagcgcaca tcaaccgctg
      241 cgcccagggc gtacggcgct atcttttcgt acccaacgca cagctctcct cgctcgagcg
      301 atcgctgttc ctggggactg ggttttatca tccctttaag aacgaacggc tcaccaaggg
      361 ccggatcagt tcgggatttg gcaatcgtat cgatcctatc caaaagtcga tcaagttcca
      421 ttcgggcgtc gatatcgcct gcccggtggg aacgcgcgtg tatgctgcga gaagcggcga
      481 ggtcgtgttc gcgggaacgc ggggcggata cggacagctg attatcatca agcacccgca
      541 cgactactac agctactacg ggcacctcca gcgaatcatg gtgaggaccg gcgaccgcgt
      601 caccacgcgc cagcagatcg cgctgtcggg caatacgggc cgcaccacag gcccgcatct
      661 gcactttgag atcaggaaaa atacaaagcc ggtcaatccc gacgttctgg cgcggctccg
      721 ctagattcgt taatcgatcc catccagcag cgtgtagaga tcggccccgt cctcgggata
      781 ttcggcggtc aggaaggtca ccagcagctg catctcccgg ttcttgaacc gctgcagaac
      841 ctcgttgcgg atggtgttgg cgaggggcac ggagaacttc ttgttctttc cgggaagcac
      901 gatgagaatc ttgttgcggt cgtatcggat cgcgaaatcg ggatccgaga ggcgctcgac
      961 gatgatcccc tcgagcaccg cgatgagctt tttggcctcg accagaccga agagatgaag
     1021 ataccgtttg aagttcttaa ttgaaaagag caccagcgta agcgggatct ttagactctg
     1081 cgccttgagg atttcacgat tgatccgctt gaggacgagc tcgatgttgt cgacgtagcg
     1141 gttctcctcg aaatccatcc gcatggtcat caacagatac ggcaccagaa aggtcgagag
     1201 gcggccgatc atccggtcga tctcgcgctc ccgctcgaca tccgcgatct gaaacaccag
     1261 aacgaacccg agcagcttca cgccgatctt gaagggatac acccaaaaat gggtcatgcg
     1321 ctttatggcg gattcgccga acgcgtcgcg gatgatcttc aggcggtcga agtcggcgat
     1381 ctgcgcgcga tccttgagat cctgcaccag atcggtaaac gggctgtcga aggctatccg
     1441 gacatcgtcg cccctgagtc cgatgagatc attgtcctcg gtcatgaccg gcacaaacac
     1501 gtcgttgcgt tcatcgccga tgaacaccgc gaaactttcg acactcatcg agcccagctc
     1561 gcgcgccacg atgtcgcaca gaccccgcag gctcgagaac tctatcatgc gtctggcgag
     1621 agtgtcgaca ttcgcgacat gctcagacgc ggcatgcagt tcgtcgcgtt cctcctgcag
     1681 ccggtcgatc atgttgatct tccgcatcgc gacggcgctg atctccccca tcgcggcgat
     1741 aaaatcgcac tcctcgtccg aataattgcc gatggtgatc ttgtcgccca gcaccaggat
     1801 gccgacgatc tcgcccgcat gcgacaatgg cgccaacaac cgcccgtcga tcgagatgaa
     1861 cttatagtac tcgtcgcggt gctccggatc gtccttgtag gtttcgatgt cgatgatgcc
     1921 gccgctttcc agaagcgatt tgaagatggc gccggactcg tcgaaataca gctgcttgtt
     1981 gcggatggtc acgccgcgcg aatcggcgat ggtccaccgg gcggtgtcct ccgacgagcg
     2041 aaccatgatc gacgacgagg aggcgccgat ctgccccatg atggagaaca ggatcgcatc
     2101 gtagagcgcc ttccggttct cggcgctcat gatctccttc ccgatttcaa agaggatcat
     2161 gaagtcctgt gcgcgtttgt cgggcttccc attggcgccc gcacggtccg acggctcgtc
     2221 gaccgcctcc ccgggtgcgc cgtcgacgga atcggcagtc gcctctatcg cacgcgccgt
     2281 cgagaattca tcgcgaatca cgacctgctg ctccgtggac cgttgaacgg ccggagcgcc
     2341 cttcaggtcc ggctcgtcgg tggttccaaa cggtccggta aattgcgggg cggcatgcgc
     2401 agcgcttccg gatcgtctca ccgcgccggc aagcggcgcg cttcccgcat cgacggcggt
     2461 cgcgcgcggc gtcccctgcc tctgcgactc gataacctca tgcggcgcgg ccccgtcgtc
     2521 gtccggcagc gtgaaaagat cgtcgggttc atcaacctgc ttcaggtctt cggcgtcaag
     2581 gagcgcaagc tcatcatcgc gtataacggc atcatccgcg tctgcgccgg tggcgggctg
     2641 atccgtgacc gcatcatggg ccgccgctgc gcttcggtcg ccggaatcct ccatcgccgc
     2701 ctcatcaacg aaatcggtct ccgccggtcc cgggatcgtg tcgatcaggg tctcgcgccc
     2761 ctttcgattg atctcctttt tatacttcag cgctttttcg agaaggccca tgtcacagtc
     2821 ccatttcctt cagcacgtcc tgatacaaag agaaatattc cgattcggca aaccgctgca
     2881 ccacctcttc gggaagtttc tcgaagagcc cgtcgagata ggccaggagc ttgcgcatgt
     2941 cctcgcgttt cgccgcaggc agcgtttcga caaatcgatc cacgtccgat ttattgtcga
     3001 taaaaacaac atcggcgcca atatccggcg cgccggccgc ccccgcttcc ttctcgcgca
     3061 caagatgatc gagttttcgt cgaatgtccg cgacattctc ctcgaacacg tacgcggcgc
     3121 ctgaggcgag atcctcctca atgctggcgc gctcatcggc attcaggctc tccgggctcg
     3181 gaaggcgata ctgcatctcg tccgcagact cactccgcgc ggccacgcga tcgcccccgg
     3241 ccattcccgc tttcggcgcg gcggggagaa ccacggcatc gtactcgccg gcgaaaacgc
     3301 tcttttcgat gacgccgatt tcatccgctg tgagcccgac gatctcaacc gcctgggtgg
     3361 ttttcttcgc gcgtcgcgcc ggcgcattgt ccgtgccgtc aaatccgttc ttctggacat
     3421 cgtccagaat ggcggcgtcg atgaatttaa gctcgttatc gacgaactgg tactcgtcct
     3481 cgaaatcgat aagcaggtcc tcaaaggccg gcgcggaccc tttcatcacg gacgcgatct
     3541 ttccctggtc atcggcgacg ttcgcctcct ccagcaagcg cgcgtccgat tcgaccgtct
     3601 caatgatatc ggcggtgatc cggtcgagct catcctgccg gaagatctca gcgggcgccg
     3661 cctcatcacc ccgctccaca aggccgtcgt cgatcacctg cgtccccggg aacttctcgg
     3721 ctatggcgat gatatcatgc ggcaacgatt cccgctcgcg ccggacggcg gcccgaggtg
     3781 ttaccgccgg tttactgtct cgcgactgca gtagcgcgcc ggttcccgaa tccggctgcc
     3841 tgcttttatc aagcgtatca aatacgacgt caaaatcatt accgtcatag gtttcaagct
     3901 cggcaagcgc gccgtccgtc ccggagaaca cggcatcatc ggcgtgtgtc gatccgcggt
     3961 catcctcgcg ccgtttcgat ccgcgggcca ccccggagtc cgtctgcggg gcggccggtt
     4021 ccatctcgcg cccctccgca tcgagcatga gctcctcgac cgcgccctca cctccatcga
     4081 cctcgctggc aacgtccccg tgtgcatcat catcaattac ttcaatcaat tcatccggtc
     4141 gttcgtcctt tcgccgccgg tcccgtgcag aatgcgcacg gagtgcgtca tcctcctgcg
     4201 aatcctcgac cgcgccattc tccgcgagaa atacaaggtc aacatcctgc aattcctcga
     4261 tgagatcctc ctcggtgaga aatacgacat cctcgttagc gaccgcctcg gcctcccgca
     4321 ggtcgatggc gccgagctcg gttatatcga aatcatccgg aaggcgatcg gcaacgccat
     4381 cgtccaattc gatggtgatc cgattcttct ttcctgactg cccacctgtt tcgataaaac
     4441 catcatcgac ctcgcgcctg tccttgtccc cgatgcggcg atccgcacct gagcgggcca
     4501 ggacatcatc atccggaaaa tcgaattcga gctcgtcgcc aatcctgccc cccgatgggg
     4561 gcgcatcatc cagaaacgat tcgagctccg catcggaaaa atgttcgatc cgcgcctggt
     4621 cgatgcttgg ttccgcatcc tctgacggcg catcgatgag ctcgaggtcc ccacccgcac
     4681 gcgtaacggc ctcctcgcct cgatctgctc ccggcgtgcg tgcaacggct gcgtgttcca
     4741 acgcaatcga atccgcggtg ctcaaatcgc tttcggaaag caccgtttcg atttcgttca
     4801 tgtcgatttc aagccgtgcg aactcatcct cggtgaccgg cggggtcgcg ggaccgacat
     4861 cacggacggg tgcggaaaat gcggcatcat cggctacgat ccgttccggg gctatacggg
     4921 gcgtatcatc attggcctct ccgtcgacca ccagatccat gtcgatatcg ctcgatagcg
     4981 ttgtatagtt aacggacgcc tcgaccgtct ccttctgctc ccgtcgacgc ggtcggcggt
     5041 cgacatccgc cgagagggct tcaatatcga atgagttcgg ctgataggca tcatcgatca
     5101 cacggggctc cgcacgcccc tccgtgatcc gggtctgttc ctccaggaca ccctcccacg
     5161 atccggcgcc atgatccttc atcacatcat ggtcatgggg tgatcccgcc gcaggcgcgg
     5221 cggcgtcatc atcaatatac tccgcttcgt gaagatcgcc ggagatgtcc gtgatctcct
     5281 cgaattgaat atctctcgtt tttgtatgtc gtgtatccac cgtaactacc acaccaccca
     5341 cgtcggtaca tgccgacccg ggcggaatta ggactttcca ccgttcgtat taaatgtaaa
     5401 ccgctcgata actattttcc atcgaaaaac tacttttatc catatattaa tatcggacaa
     5461 atgttgaaaa taattcaccg gaaagggata ttccatacaa tccgctctag aaacgcgaaa
     5521 ggatgcccgt ttacggggca tcctttcttg agcatgaaac cgcctcggat tgctagcgcg
     5581 tcatcgggcc gcaaccttga gacgggcctt gagcttgcga agctcaatat ccaacttcat
     5641 gcgctccttc acatcgagct tggtaaaatc attgatcttg tactcaatct cggacctgac
     5701 cagctcgtcc ttgcgcatgg cctgctcgca gataagaatg agatcgtttt ccttgatttc
     5761 gacaaaaccg ccctcaacat aaaagaagtc ggtggtgttc gccgtacgca accgcacctc
     5821 tccaacgcca agctctgaga ccagcggcgc atgattgtac aaaaagccta tttcgccatc
     5881 gtgcgcctgg acgatcgcaa attcgacctg cccctcgtac agagtgcgct cgggcgtgag
     5941 aatgctacag ttgagtttca tcgtcatagg actagcttcc cgcttgcaat ttcttcgcct
     6001 tttccactac ctcttcgata gtccccacca tatagaacgc ctgttcgggc agactgtcgt
     6061 attcgccttt tatcaggccg ttaaagctcc gaatggtctc ctcgagcttt acgtacttcc
     6121 ctggcatgcc ggtgaactgc tcggcgacga agaacggctg cgagaggaaa cgctcgatct
     6181 tgcgcgcacg ctgcaccaga agcttgtcct cctccgagag ttcgtccatg cccagaatgg
     6241 cgatgatgtc ctgcnnnnnn nnnngcaggt ccttgtagcg ctgaaggatc cgcttcacct
     6301 ccatggccac atcgtaatgc tcctggccca ccagctcggg agcgagaagg cgcgaggagg
     6361 aatcgagcgg gtccacggcc ggatagatgc ccttctcggc gatctgccga gaaagcaccg
     6421 tgctggcgtc gaggtggatg aacgccgtcg ccggcgcggg gtcggtcagg tcgtcggcag
     6481 ggacataaat cgcctgcacc gacgtgatcg aaccgtattt tgtcgaggtg atccgctcct
     6541 gcagctcgcc catttcagta gacagcgtgg gctggtatcc caccgcggag ggcatccgtc
     6601 ccaggagggc cgacacctcg gaaccggctt gcgagaaccg gaagatgttg tcgataaaca
     6661 gaagcacgtc gcggccggac atatcgcgga aatattcgca catggtcagc gcggaaagtg
     6721 cgacgcggag gcgcgctccg gggggctcgt tcatctgtcc gtacaccagg caggccttgt
     6781 tgataacgcc cgattccttc atctcgagcc agaggtcgtt tccttcgcgg gtgcgctccc
     6841 ccacgccggc gaacaccgaa tacccgccgt gctgctgggc cacgttgttg atgagctcca
     6901 tgatcacgac cgtttttccc acgccggcgc cgccgaacag gccggtcttg ccgcccttga
     6961 tgtagggcgc gagaaggtca atgaccttga tcccggtttc gaagatctcg gccttcggct
     7021 cgagctgttc gaacttgggc gggtgctgat ggatcgaacg ccggtccttc gtgttcaccg
     7081 cagggccgtt gtccaccggc tgaccgagca ggttgaagat gcgccccagg gtttcctccc
     7141 ccacgggaac ggaaatcgga gcgcccgtgt cggtgatcgg cgtaccgcgg cgaatgccgt
     7201 cggtcgaggc cagcgcaacc gcacgcacgc ggcttccgcc gagatgctgc tggacctcgc
     7261 aggtgatgag tgacttcgcg cccataacgt caatctcgag cgtcagggca ttatacaatt
     7321 ccggcaaatg gccttcggga aattcggcat ccagcgtcga tccgataacc tgtacgacct
     7381 ttccaacgtt ctgagccatg ggtaaactcc tttatgatag tatcttcgca accccgctcg
     7441 atccgatcat tccacatgcc gccgatcgcg gcaccatggc ggggtccgca caacaaatct
     7501 cacatcatat ctagccgagc gccgccgctc cgccgacgat ttccgcgatc tccgtcgtaa
     7561 tcttcgcctg ccgagcgcgg ttataggtga tggtcaggtc acgaatcatg tccgtggccg
     7621 catcagtcgc gttcttcatg gaaacccgcc gcgcgaactg ttccgaaaag ttcgattcga
     7681 gcagcgcgag aaacacctgc gccttcacat agagcggaag gagcgaggaa aaaatcctat
     7741 aggggttggg ctcgaagatg tactgtgggg tgaattcggg cccgacgtcc tcgacgccga
     7801 cgcgctccgg cttgattggc agcagctgca cgatcaccgg cttctgtgaa gatgccgata
     7861 cgaccttggt gtacgacagg tacacctcat gcgcctcgcc gtattcgtac agattgatga
     7921 gttcctgcgc aattttcgcg gcctctgcga aggagacctt gtcctccatg ttgagagagg
     7981 acttatacat cggctgaccg gtgaagttaa agtagccaat ggcctttttt ccaatagcat
     8041 agatctgaac gtcgcggccc tcctcgatct cgagccgctt cttgaaggcg atggtgttgt
     8101 tgatcacgtt gttgttaaac ccaccgcaca gccctcgatt gccggtcacc gtgaagacga
     8161 gcgtccgctt tgactcgggc cattcctgca tcaacgggtc gtaaatgttt tcgacgccgg
     8221 aattgcggag attcgcgatg acctggttca gcttctgcac aaagggctgc gacatgagta
     8281 gccggccctg catcttcttc atcttggccg tggaaaccat ctccatggtc ttggtgatct
     8341 tcttggtgct tttgaccgat tctatgcgct tcttaatctc tcgttgtgtt gccacagctt
     8401 attcccctgt gcttgagtgt tccctggaac gccccaccct atgcccgacc ggcgaggtag
     8461 ccctcggcat agtcactgca gaccttcttg tacctgtcga tgctcttcag ctcgcccgtc
     8521 ttgctgatct catccaggat gtccgcctgc tgttcgttca agtatttgat gaacttgacc
     8581 tcaaagtctt tcaccttttc caccgggacc ttgtcaatat aaccctgtgt tccggcgaag
     8641 atgacggcca cctgttctgc caccgacatg ggaacatact ggccctgctt gaggatctcg
     8701 ataaggcgcg cgccgcggtc gagctgctgc tgcgtcgccg catcgagctc ggtacccatt
     8761 tgcgcaaaag cctcaagttc acgatactgg gccaggtcca aacgaagcga accggcatac
     8821 ttcttcatgg ccttgatctg cgcattgccg cccacccgcg acaccgagat gcccacgtca
     8881 accgctggtc ggataccgga ggcgaaaaga ttgggcacga ggtaaatctg accgtcggtg
     8941 atcgagatca cgttcgtggg aatgtacgcg gagacctcgc cttcctgggt ttcgatgatc
     9001 ggcagcgccg tcattgaccc gccgcccatt tcatccgaca acttcgctgc gcgctccagc
     9061 aaacgcgagt ggcagtagaa gatgtcgccc gggtacgcct cgcgtccggg aggacggcga
     9121 agcaggaggg agagctgccg gtaggcgctg gcctgcttgg taaggtcatc atagacgcac
     9181 agcgtatgac cgcctttttc atacatgtag tattcggcca tcgcggcgcc ggcatagggt
     9241 gcgatgtact gcaagggcgc cgcctcggcc gcgccggcga ccaccacgat ggtgtattcc
     9301 atggcgccat gctgctcgag cttctcgaca acggcggcaa ccgtagagtc cttctgtccc
     9361 acggccacat agacgcagat tacgttcttt cccttctggt tgatgatggt gtcgatggcg
     9421 atggcggtct tcccggtttt gcggtcgccg atgatgagct cgcgctggcc gcgaccaacg
     9481 ggcgtcatgg agtcaatcga cttgatcccg gtctggaggg gctccttcac gggctgccga
     9541 gaggcgatgc ccggcgcgag gaattccatg ggtcggagcg ccgtcgtatt gatgggcccc
     9601 ttgttgtcga tcggaatacc gagcggattt accacgcggc cgaggagggc atcgccgcag
     9661 ggaacggcaa gcacgcgatc gagccgcttg acggtgtggc cttcctcgat ggtgaggtag
     9721 tcgccaagaa ccaccgcccc gatggagtcc tcttcgaggt tgaacaccag gccgtaggcg
     9781 ccgttctgga attcgagcat ttcgccggcc atcgcattgg acaggccgta cagccgggcg
     9841 ataccgtcac cgacctgaat gaccgtgccg acctcgctga cattgaactc cttcttgtat
     9901 ccctctatct ctttcctgat tattgatttg atttcttccg ttttaatctt cataggctct
     9961 atcacccctg accttgctgt ttaaaagatt atgtcgaata tttttcaggt cattcgccat
    10021 cgacccgtcc aatattatat cgcccatgcg gaggatgatg ccgcccagaa tcgactcgtc
    10081 gaccgcatca tccaaaatta tgttcttttt aagtttttcg gaaagggagg cctgcacctt
    10141 gtccaggaca tccttgccga gcttcatgct cgatatgacc gttacgcgct ggcggttgtt
    10201 cacgacatcg aggagctcaa cgaacgcttc gttgatgctt ttcaggagcg cctgccggcc
    10261 attgtcgacg aggaccttta gaaggttaac gatcgtttcg gaaaactcgc cgccaaacac
    10321 gcggtcgatg aactctttct tcgcgtcggg agaaatgccg ggggcgttca gataaatctc
    10381 gacatcgcgg tcctcgctga tgagtcccac aataaacccg agctcctcct ccaaccggtc
    10441 aaggatcttc ttttcctgtc cgatctcaac gagggcattg gcatacacat tagcgaccac
    10501 attgcctgcc acagtactaa ccctaccatt taatatgtgc gcaaaaacac taaggacagc
    10561 tcagggttac tgaactgtcc ttattttggt caacgcttct tcgacgatcg ccttctgatc
    10621 ctccggtttg aggttcttga cgataatctt tgacgcaatc tcggttgaaa gaagggcgat
    10681 ttcattcttg agctcgccga gagccttatc cttcgaaagg gttatctccc gtttcgcgtg
    10741 ctccatgatc ttctgcgcct cttcgttcgc cttttccagg agaccgcttc gcaaccgttc
    10801 agcgtccgct ttaccatcgg cgatgagctt ggccgcttcg tttttcgcgt tgttcatcat
    10861 cgccttgtgg tcttcgaaga tcttctcggc ctcaatgcga ttcttctccg cggtttcgat
    10921 gtcattgcgg accttctcgg cccgcaaatc gagggcctcc accaacggct tccaggcggc
    10981 cttccacaga atgagcacca acacgataaa ggtgaccagc gtccacagat agaggcccgg
    11041 atcgactttc aacaaccctt ctcttattgc atccataccg aactacctga tagtgaattt
    11101 tttactaccc gaatctcccg ccgtacctac ttcgtcttcg gctgctcgac agcggctggc
    11161 gctttttcgg caacgggcat gataatcggg tttttggtgg ccagcatgaa cgtaataacc
    11221 agcgcgaaga aggtgaaacc ttcaatgagc gctgccgcga tgatcatcga cgttctgagg
    11281 tcgccgctga gctcaggctg acgagccatg ccctcgatag cgccgctggc gagcttgcca
    11341 atgccaagcg cggcaccgaa cacgatcagc ccggccccaa aaccagctcc aagataagac
    11401 aatcccagat aataagtgtc cattttttcc tcctaccgga ataattaagg tgcgtcaccc
    11461 tgtaacaggt aacttaaaaa tttgcagttc attcaattga ccagggcagc ccgcacgcgc
    11521 cggaacggat ccggcgactg gcgatagcgg cgatggaaaa ttaaaaaaac ctattcaaac
    11581 acgcgcacga tttagtgcgc gtgtaatgat gcgccgataa acatcgcgga aagcgccgta
    11641 aagatatacg cctggataaa cgcgaccaac agctcgagca tgttgatcat caacgcgccc
    11701 gcgaccgacc cgatgccgac cagcgcgctc ttgaacatca tcaccagaaa gatgaagatg
    11761 atgatcacga tatggccggc ggtcatgttt gcatagagac gaacggtgag cgcaaacggc
    11821 ttgatgagaa gacccatcag ttcgatcacc cacaacagcg ggaagagcgg cagcggcacg
    11881 ccgcccggaa cgatgccggt gaaaatccac tgcggcccct gcttgatcgt gccgatgacg
    11941 atcatcccca gcagggtcgt tgacgccagt gcgccggtta ccgcgaggtt gcccgtggcc
    12001 gtcgacatat atggaatcat gccaagcaga ttgcagaaga gcacgaaaaa gaacgtggtg
    12061 cagaaccagg gcatcgcaat ctttttgtag tgatggccga agttgggctc gacgatctca
    12121 tcatgcacga agccgacgag gacctcccac atgttgaccc acttggagct cgacccgtat
    12181 tttgcgtttt tgattttgcg tgcgacagga atgaagatga tgatgcaaat gaacgcggcg
    12241 agccacatca tgagcaccaa cttggtgatg gccagattgc tcttgagccc gagcacctcg
    12301 aaggtgatcc cgccgaggac cgtgttgacg ggctcgagga tcttcgcgat gaaattgccc
    12361 tcgacgacat ggtcggtgag gtggtgcatc acgacatcgg aaaccgattc ttccttgccg
    12421 ccgtgtccgc cgccggcgcc aaccaccata ttgacaagac taataagctt cagcataacg
    12481 gatcacacct gccctcgatt gtccttatcg ctcgcgcgcc ggcacggcta cagccacttt
    12541 gtccgcttct cgccgtcctg cgaatctgcc gcgggcttgt tctcaaggaa ccgatcgatg
    12601 ttcctgagct cacggatgag attgtaaaac cccgccgtca tgcccagcac tacgctgagg
    12661 ataaacagcc acggactgct acgaaaatgc tcgtcggcct tgtaaccaac gtaaataaaa
    12721 aggagtatcg ttattgccaa ttgcagaccg atcgtcgagt acgccatgac cgatcgtgca
    12781 gtccttttcg ttaacaaaac gcgtataaga gaaccgttgt aatcgttaca cccaaactga
    12841 ttacattcat caatcaaggc gttatttgtc aagaaaatgt agaacttttt ttgggcgatc
    12901 gcgtgaaaaa tggcgaccac cgccccgcac cgccccgccg gacgtcaaac gtttttcccg
    12961 cgatttttac ggggcggaat cagcaccggg cggattggag ttttttatta attaagcaac
    13021 gccgcgcacg cggacgataa ttgaatcgta ccaaaaaaca cgccgccggg cgggagcgtc
    13081 cggacgacga ccatgcgggt tcgaccatga aattgtccat taaactcatc gagtgtgacc
    13141 gggaatacat cgtctcatgc ccggagctcg acatcaattg ctacgcaccg acgaaaaacg
    13201 aggccttgcg gcgcatcgtg accgtgatca gcttctatat cgattccgcc aaggagctgg
    13261 ggctcgaggt cgggggcatc gaggcgatca acgtggaggg gcttcc
//
LOCUS       DAORHF010000089        13235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3755_length_13235_cov_4.922534, whole genome shotgun sequence.
ACCESSION   DAORHF010000089 DAORHF010000000
VERSION     DAORHF010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3755_length_13235_cov_4.922534"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PKJ16_09070"
     CDS             <1..792
                     /locus_tag="PKJ16_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV47180.1"
                     /translation="LDGGAMFGVAPKALWGRFYEADADNRIRLACRALLVAGKGIALL
                     VEGGAGDVFDGNEKLMRSYAIESTNRLDSALGAIGYAPGDITHAVFSHLHWDHAGTAC
                     RRERDGRFVPRFPKARYVVQRGEWDAALSKDPLTAPSYVPEALVPVRESGQLECIDGD
                     GRINDDIRLVVTGGHTENHMALMLESGGERLVFWGDIIPTSHHIHLPLIMAYDRFPVD
                     TYRVKERLLADACRENAVSAFPHDCEVGFGRISFDGKRYRLAEGL"
     gene            898..1455
                     /locus_tag="PKJ16_09075"
     CDS             898..1455
                     /locus_tag="PKJ16_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47181.1"
                     /translation="MHDTIDISGITTAVLFTGLGVMAPILFHALGLGAIFLPMYLPLA
                     IGAFLLGRADAALMGACTPLVSALLTGMPPFYPPIAVTMAVQLGAMCLAISLVSHRLE
                     ALSRGAIVLTLVAAFAVERSLMALLYRFVMPLFGVSGKVFTAYDLVKSLPGIAIIFAA
                     APFAVPKAAQLLRRFSIRPYERSGD"
     gene            1465..2082
                     /locus_tag="PKJ16_09080"
     CDS             1465..2082
                     /locus_tag="PKJ16_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV47182.1"
                     /translation="MDDRFARKTEYFNRLAPNWDDIVGNDGARQERLAEVFARVPVRR
                     GMRVLDVGCGNGVLVPHIVARVGSEGSVCAIDAAPAMIERARTLHGRHANVRWIAGDI
                     ETCGLPEGAFDIILCFAVFPHVADKARALAAMRALLAPGGRLVIFHLADTASLNSFHA
                     SLDAPVHDDMMPGRDELEALFADTGFRLEAYEDAPGLNYVEAAPC"
     gene            2076..2744
                     /locus_tag="PKJ16_09085"
     CDS             2076..2744
                     /locus_tag="PKJ16_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47183.1"
                     /translation="MLTLCKIAALGLAVASFVSCRPFPQPAILFPTAAGVVLAVMGGE
                     PAREQLRATLPAALFALSFSLFTVVSSLAAGALNAGEAAGNAAAVVYVFLAARAGMRW
                     IGRRGFGDLVRIVPSLRISLYAVLVFNALAAGARRNRLIRHQLMARFGSVRGHRWRLA
                     RYYAHNVILKELYTAHLNQAAIALRVNGPLPAAPFAPLTARDLAAALAAMSLAAAGFA
                     AVTP"
     gene            2741..3442
                     /locus_tag="PKJ16_09090"
     CDS             2741..3442
                     /locus_tag="PKJ16_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV47184.1"
                     /translation="MTAHAVEIADLRFSYPDGTEVLRGITLAVPAGSRLALVGANGSG
                     KTTLLMHLAGLLDGPGRIALFGVERTRASAPELRRRIGYLFSSVAYQFIMPTVLDDII
                     LSDPRPELPYDERRERAFAWLRRFRLEAYAGRYPLDCSTGEMKRAALAGVLAREPDCL
                     LLDEPLASLDRPSGDELLRLLAEVPTTMIVATHQRAIVERLGANVALLDSGVVAGTFT
                     ARQALRTRAVTNLLW"
     gene            3560..5782
                     /locus_tag="PKJ16_09095"
     CDS             3560..5782
                     /locus_tag="PKJ16_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV47185.1"
                     /translation="MSKKIVVLLLMAVTALPLYAQTDTTGTPADKKDQSAFSGTEIVV
                     TEKKIHPGVVSVIKGEEVKKSTATDLINLINEKVPSFYTPERGVMGFGVASNGAGRVT
                     IRGMGLSSWSLTAPTSPTHGLPILINGVDTTMGIMGHPVADVFAMKNVEHIEVLHGPQ
                     PVLYGASAVGGVINVITKRQEKEGFETEITASYGTFNTTDDSLVHRGKIGGFDYAISY
                     NFRHTDGHREQRINNTTFTARYQDHNATAHLGYEFGKHIYASLDGYAMQMTAHDPGGV
                     DSALDGEEYPYPSDRLEVFRIDRNGGLFSIRNSFNMLDGSLIAYANSGHHRSWMPAYD
                     EEENKNLAAYKAKSNSTFKSNDVSYGVKLKETLKNIIPGNRITAGFDIRQVGGTAVNP
                     QKYEGYPQRRQIDDNRYVQYDPYYYVKNKYITQQSYFGLVEQGLFENIIVLTAGARHT
                     WDSASGDFDAWQGGVIVNPHKTTKVHASGAKGFILPEIRELYFKFAAQDNTIQETEFA
                     LKPETYYIYEAGIEQTIADFTLALTGYRIFSYDKWYKKSLLASDPYPKNPTGPAMKGS
                     AWDNMDKFYYSGLEASAGYKYEKMLDVSVGYSYIDNEWNNQVLPYVPTHKVVANIGLA
                     IADFFAGVSGEYIKELYWENPVPDDAANQSQTDKYGDTCGNRWRKEIPAYYVINAKLA
                     YTFMKNYRAFVNLYNITNQRYTAMVVKANVGTDASPNYRGFDYPMPGFHFLAGLSATF
                     "
     gene            complement(5843..6847)
                     /locus_tag="PKJ16_09100"
     CDS             complement(5843..6847)
                     /locus_tag="PKJ16_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HNV47186.1"
                     /translation="MKRIEITALLGIAALIATLITAAPALCWTTELNTNGIRVYSRQP
                     AKFPFKEYRGIVVIDAKLEVLGEVLRDPAAFPQWMANCTSASIVTKIDRNNMTLRIVL
                     SMPAPVSDRDIVVEAKTVYDLATARGIVKLRALADSAVPADTRFVRITTFAGQYLLEY
                     IGREKTRVVYTLRTDPRVGMPAFVVNSYVKDYAYKTLDGMRRMAAMGKYVAAGAVSPD
                     RPIFDKTLKDPAALRRIMNARMREFIRDPLVSEIVATDAGIVNIVMRGGGSFASVKES
                     FKRGCALYLAKKHGYDARTVEAAVNNTEFEREILNAILYGSEAGRFDVDSWIKKRIGK
                     "
     gene            7070..7444
                     /locus_tag="PKJ16_09105"
     CDS             7070..7444
                     /locus_tag="PKJ16_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47187.1"
                     /translation="MSWSPKVFIVAALLVAVAIYAMFCSSRDISGTYSLVKCVDVDST
                     EIGERAGAAGTIIIKNLSGRRFAIEHAGGSNDGTRLIGELEDTRLIVKIGTSPVVYAF
                     ASGSRSFEYNGYSGRCVYEKSN"
     gene            complement(7426..8145)
                     /locus_tag="PKJ16_09110"
     CDS             complement(7426..8145)
                     /locus_tag="PKJ16_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47188.1"
                     /translation="MTAGHDASSEARALYRAGADQADFDFARGVPALLAADNAECVFL
                     AGRDWREFDYARGLDRLIALGSAEFIHKAGIFWKRFDYARGCRALFDLADPEYVYRAG
                     RFWREFDHAGAIALFSALGLPRYLYYAGKEWKRFDYEKGFDALVALGSAEFIFYAGAH
                     WPVFDYREGFERLLRAREAEFIYKAGTLWREFDFARAWAVLETLVIEGAEWRGRAFTH
                     PRWRDALRRIWRDAAAQFDFS"
     gene            complement(8142..9569)
                     /locus_tag="PKJ16_09115"
     CDS             complement(8142..9569)
                     /locus_tag="PKJ16_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47189.1"
                     /translation="MSAPVDIALETLWRRSRFASYFYQQVQFFPDAAIPTLALTVLQS
                     RLTLAYRPDFVESLHAEQFIGLLIHEMMHAVLNHDHRALPDESPYLQNVAQDMAVNSY
                     IVAHHTTFFSRTGDYAADTPELLLPPGLPVIPHAFYRATGIGDPSWEDVFRWLKGLSP
                     EERRAHIDSGPIDPGIPLDAGAHDGARLDERIDTAPLFRAPDDERSRFDWSGLRGIVF
                     IDERDETLPTGVHLFHDRAAADAITAKRTRTLSLAEADRDCADERAFQEIRGIINTTT
                     EADIDSWERLLKSTIDFAAQSNEWTYTYGRFNRRYFANGIYAPGRIFREREVITVAVD
                     VSGSMVMNPADIETAFGVIEQLAEKYRVNLLCLDENLFVPEKRDDAFVAARDAGRPYR
                     YQKGDWRFIRTGAGGTTFFAPLFNSYLAGHTEMLLVITDGYIYDIAELREYSPTIWLI
                     AEGRDEPFVAPFGQAVRIRAKRGRR"
     gene            9669..9866
                     /locus_tag="PKJ16_09120"
     CDS             9669..9866
                     /locus_tag="PKJ16_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47190.1"
                     /translation="MYITKDGKKLEDVIRTAIDDSEVTMAEYEEIINVAFEDGKLDAH
                     EKALLKEFKSLIAQNVVKRVP"
     gene            complement(9961..11115)
                     /locus_tag="PKJ16_09125"
     CDS             complement(9961..11115)
                     /locus_tag="PKJ16_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47191.1"
                     /translation="MKKTPVIVSARQLEEILEGYFDAGWRLNYAIKVLGHPGVGKSAI
                     VKAVAERKGMHFIDTRLAFKENIDLGGYPVPDHEARRMIYYRPRFIPPERVPDGAAGI
                     VWFLDESNRAHPTVIQTLFQIITERTCGEHALPERTAIVLAGNLGEEDSTTITNFDDA
                     ALDGRLAVFQLRPAAAEWLPWATREGVHPSVMRYISVFPDRLWDERAINPNPRGWHQV
                     SCAISDAFGLADEDALAARLAAEPSGPLALTITALIGASAAADFVAQICAPRRLVTAE
                     VIAGDRARLRELADDAIPAEDVLWALSGAIDHLRETRARGAATPTDAELDEIANVLMF
                     AGHARADFRISFFYRLLRDCGIFAQAPAALHRLPDTAMGAALLERFERIL"
     gene            complement(11112..11738)
                     /locus_tag="PKJ16_09130"
     CDS             complement(11112..11738)
                     /locus_tag="PKJ16_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47192.1"
                     /translation="MNKTTKKKSTLSELKSKERQDRKDLIIDAAERVFATRPFNRVSM
                     REIADEAGIAASSIYTYFPNQEALFVEATVRDSTTFLDELGKITAGGPVTADSFDRLI
                     DTFFDFISTHDSYFRMMVVFMTHGQLNPDSLLRVNALVGRGLDIFEQVISDLGYRGDT
                     RLLAHYFFAMMNGILVNYRKYEGRREEEVTAFMKKVAKVFKDLLLKSI"
     gene            11918..>13235
                     /locus_tag="PKJ16_09135"
     CDS             11918..>13235
                     /locus_tag="PKJ16_09135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV47193.1"
                     /translation="MILLNPHKHNRTYKDEKSKELMQKTIDFFEKKGLKQIKKDWHEK
                     KWNYDFVEFLKETQAFAVLMTPSGYGASDSRWDTYRNSAFSEITGFYGVTYWYTWQVS
                     MLGLCPIWNGSNEEVKHKTAKLLQEGGVFAFGLSEKEHGADIYSSDMMLYPEGDGKYR
                     ANGDKYYIGNGNEAALVSTFAKMADTGDYVFFVVNSKHPKYECIRNVVNEQNYVAEYA
                     LHDYPITDADITERGPKAWDDMLNTINVCKFNLGWGAIGLCTHSLYESIDHAANRNVY
                     GKFVTDFPHVKRLFVDAYCKLVAMKLFAERAIDYMRSANENDRRYLLFNPMVKMKVTR
                     EGEEVMNILHDIIAAKGFENEPFFEIAKHELPMLPKLEGTVHVNMALIVKFMANYFFN
                     PKEYPEVPKRDDLANDEFMFRQGPTKGLGKVQFHDYNIAYNSVNLPN"
BASE COUNT         2404 a         4256 c         4202 g         2373 t
ORIGIN      
        1 ctcgacgggg gcgcgatgtt cggcgtggcc cccaaggcgc tgtggggccg cttctacgag
       61 gccgacgccg acaaccgcat ccggctcgcc tgccgcgcgc tgcttgtcgc ggggaagggg
      121 atcgcgcttc tggtcgaggg cggcgcgggc gacgtcttcg acggcaatga gaagctcatg
      181 cgctcctacg cgatcgaatc gaccaaccgg cttgattcgg cgttgggcgc gatcggatat
      241 gcgccggggg acatcaccca tgccgtcttc tcgcacttgc actgggacca cgcgggtacg
      301 gcctgccgcc gggagcgcga cgggcgtttc gtcccgcgct ttccgaaggc gcgctacgtc
      361 gtccagcgcg gcgagtggga cgcggcgctc tcgaaggatc cgctcaccgc gcccagctat
      421 gttcccgagg ccctcgtccc cgtgcgcgag agcggccagc tcgaatgcat cgacggcgac
      481 ggccggatca acgacgacat ccgcctggtc gtgaccggcg ggcacacgga gaaccatatg
      541 gcgctcatgc tcgagtcggg cggcgagcgc ctcgtcttct ggggcgatat cattcccaca
      601 tcgcaccata tacacttgcc gctcatcatg gcctatgatc gcttccccgt ggacacctat
      661 cgcgtgaagg agcgcctcct cgcggacgcc tgccgagaaa acgccgtctc cgcctttccg
      721 cacgattgcg aggtcggctt cggccgcatc tccttcgacg ggaagcgcta ccgtctcgca
      781 gaggggctct gacccctccc gggcccccgc ccgagaaaaa ttcttgtcag ccggatgggg
      841 ccgcgctaag gtggcacaga tagggcaacc gtgccacttg cgcgatccgg gaccagcatg
      901 cacgatacaa ttgacatcag cggaatcacc acggcggtcc tttttaccgg gctgggcgtg
      961 atggcgccca tcctgtttca cgccctgggg ctgggcgcca tcttccttcc gatgtatctt
     1021 cccctcgcga tcggcgcatt cctgctgggt agggccgatg ccgcgctcat gggcgcgtgc
     1081 acgccgctcg tgtccgcgct cctcaccggc atgccgccgt tctatccgcc catcgccgtc
     1141 accatggccg tgcagctggg cgcgatgtgc ctggcgatat cgctggtgtc gcaccgctta
     1201 gaggccttga gccgtggcgc gatcgtcctc accctcgtcg cagccttcgc cgtggagcgt
     1261 tcgctgatgg ccctgctcta caggttcgtg atgccgctct tcggcgtttc cggaaaagtg
     1321 tttaccgcat acgatctcgt gaagtcgctg ccggggatcg ccatcatctt cgccgccgcg
     1381 ccgttcgccg tccccaaggc cgcacagctc ctccggcgtt tcagcatccg gccctacgag
     1441 cgaagcggcg actgaggccc gaccgtggac gatcgcttcg cgcgcaaaac ggagtacttt
     1501 aaccggctgg ccccgaactg ggacgatatc gtcggcaacg acggcgcgcg gcaggaacgg
     1561 ctggccgagg tgttcgcgcg cgtgcccgtg cggcgcggca tgcgggtgct cgacgtgggg
     1621 tgcggcaacg gcgtgctcgt tccgcacatc gtcgcgcgcg tggggagcga gggttcggtg
     1681 tgcgcgatcg acgccgcccc ggcgatgatc gagcgcgcgc gcacgctcca cgggcggcat
     1741 gcgaacgtcc gctggatcgc cggcgacatc gagacctgcg gcctgcccga gggcgccttc
     1801 gacatcatcc tgtgcttcgc cgtgttcccg cacgtcgcgg acaaggcgcg ggcgcttgcc
     1861 gcgatgcgcg cgctcctcgc gccgggcggc cggctcgtca tcttccacct cgccgacacc
     1921 gcgtcgctca acagcttcca cgcctcgctg gacgcgcccg ttcacgacga catgatgccc
     1981 ggccgggacg agctcgaggc gctcttcgcc gataccggtt ttcgcctgga ggcctacgag
     2041 gacgcgcccg gcctcaacta cgtcgaagcc gctccatgct gacgctctgc aaaatcgccg
     2101 cgctgggact ggcggtcgcg tcgttcgtat cgtgccggcc gtttccgcag cccgcgatcc
     2161 tcttcccgac ggccgcgggg gtcgtgcttg cggtcatggg cggcgagccg gcgcgtgagc
     2221 aattgcgcgc aacgcttccg gcggccctct tcgcgctcag cttttccctc tttacggtcg
     2281 tctcgtcgct ggccgccggc gcgctcaatg ccggggaggc ggcggggaac gcggccgccg
     2341 tcgtgtacgt gttcctcgcg gcgcgcgcgg ggatgcgctg gatcggccgc cgcggcttcg
     2401 gcgacctcgt gcgcatcgtc ccgtcgctgc ggatctccct gtacgccgtt ctcgtgttca
     2461 acgccctcgc cgccggggcc cggagaaacc ggcttatccg ccaccagctc atggcgcgct
     2521 tcggttccgt gcgcggccac cggtggcgcc ttgcgcgcta ttatgcgcac aacgtgatcc
     2581 tcaaggagct ctacacggcg cacctgaacc aggccgccat cgcgctgcgg gtgaacggcc
     2641 cgctcccggc ggcgcccttc gctcccctca cggcgaggga cctcgccgcc gcgcttgcgg
     2701 cgatgtcctt ggctgcggcc gggttcgcgg cggtgacgcc atgaccgcgc acgccgtcga
     2761 gatcgcggac ctgcgctttt cgtatcccga cggcactgag gtgctgcgcg gtatcaccct
     2821 tgccgttccc gccggttccc gtctcgcgct ggtcggggca aacggctccg ggaaaaccac
     2881 gctgctcatg cacctggcgg gcctgctgga cggtcccgga cggatcgcgc tcttcggcgt
     2941 ggagcgcacg cgcgcgagcg cgccggagct gcgtcggcgc atcggctatc tcttcagcag
     3001 cgtggcctat cagttcatca tgcccacggt gctcgacgac atcatcctga gcgatccgcg
     3061 accggagctg ccgtacgacg agcggcgtga gcgggcgttc gcctggctcc ggcgcttccg
     3121 tctcgaggcc tatgccgggc gctatccgct cgattgttcc accggcgaga tgaagcgcgc
     3181 cgcgctggcc ggcgtcctcg cgcgcgagcc cgattgtctg cttctcgacg agccgctcgc
     3241 gagcctcgac cgcccctccg gcgacgagct cctgcgcctc ctcgcggagg tgcccacgac
     3301 catgatcgtc gcgacccacc agcgcgccat cgtcgagcgg ctcggcgcca atgtggccct
     3361 gctggactcg ggtgtggtcg ccggcacgtt taccgcccgc caggcgcttc ggacccgcgc
     3421 ggttactaat cttttatggt agggtgatga tagtgcgctc ctcgtgatat cacgaagggc
     3481 gattttttat attgacaggt aacacgataa taaatcgtgt gttactcaat gcatacagtt
     3541 tttgctccag ggtgatacca tgtctaaaaa gatcgttgta ttattgctga tggccgtaac
     3601 ggctctgccg ctttatgcgc agacggatac aacgggcacg cccgccgaca aaaaggacca
     3661 atccgcgttt tccggcaccg agatcgtggt gaccgagaag aagatccacc cgggcgtggt
     3721 gtccgtgatc aagggggagg aggtgaagaa gtccacggcg accgacctca tcaaccttat
     3781 aaatgaaaag gtccccagct tttacacgcc cgagcgcggg gtgatgggct tcggcgtcgc
     3841 ctccaatggc gcgggacggg tgaccatccg cggcatgggg ctttcgagct ggtcgcttac
     3901 ggcccccacc tcgcccacgc acggcctgcc catcctcatc aacggcgttg acacgacgat
     3961 ggggatcatg ggccatcccg tggccgacgt cttcgcgatg aagaacgtgg agcacatcga
     4021 ggtgctgcac ggcccgcagc cggtgctcta cggcgcgagc gcggtgggcg gcgtgatcaa
     4081 cgtgatcacc aagcgccagg agaaggaggg cttcgagacg gagatcacgg cctcgtacgg
     4141 gaccttcaac accaccgacg attcgctggt gcaccgcggg aagatcggcg gattcgacta
     4201 tgcgatatcg tacaactttc gccacaccga cgggcatcgc gagcagcgga tcaacaatac
     4261 gaccttcacc gcgcgctacc aggaccacaa tgcgacggcg cacctgggct acgagttcgg
     4321 gaagcacatc tacgccagcc tggacggcta cgcgatgcag atgaccgcgc acgatccggg
     4381 cggcgtggac agcgccctcg acggcgagga gtacccgtat cccagcgaca ggctcgaggt
     4441 cttccgcatc gatcgcaacg gcggtctttt ctcgatacgc aattcgttca acatgctcga
     4501 tggttcgctc atcgcctacg ccaacagcgg ccatcaccgc tcctggatgc ccgcctacga
     4561 cgaagaggag aacaagaacc tcgccgccta caaggccaag tcgaactcga ccttcaagtc
     4621 gaacgacgtg tcctacggcg tgaagctcaa ggagacgctc aagaacatta ttcccggcaa
     4681 ccggatcacc gccggcttcg acattcgcca ggttggcggg acggccgtca acccgcaaaa
     4741 atacgagggc tatccgcagc ggcggcagat tgacgacaac cggtatgttc aatacgatcc
     4801 gtattactat gtgaagaata agtacatcac gcagcagtcg tacttcggcc tcgttgaaca
     4861 gggcctcttc gaaaacatca tcgtcctcac tgccggcgcg cggcacacct gggattccgc
     4921 gagcggcgat ttcgacgcct ggcagggcgg ggtgattgtt aatccgcaca agacgacgaa
     4981 ggtccacgcg agcggcgcca agggcttcat tctgccggag atccgcgagc tgtacttcaa
     5041 gttcgccgcg caggacaata cgatacaaga aaccgaattt gcgctcaaac cggagaccta
     5101 ctatatctac gaggcgggga tcgagcagac catcgcggac ttcacgctgg cgctcacggg
     5161 ctatcgcatc ttctcgtacg ataagtggta caagaagtcc ctccttgcat ctgacccgta
     5221 tcccaagaac ccgaccggtc ccgcgatgaa gggatcggcg tgggacaata tggacaaatt
     5281 ctattacagc gggctcgagg cctcggcggg gtacaagtac gagaagatgc tggatgtgtc
     5341 tgtcgggtat tcgtacatcg acaatgagtg gaacaatcag gtgctgccgt atgtgcctac
     5401 gcacaaggtc gtcgcgaaca tcggcctcgc catcgccgat ttcttcgcgg gcgtgagcgg
     5461 cgagtacatc aaggagctct actgggagaa tcccgtgccc gacgatgcgg cgaaccagtc
     5521 acaaaccgac aaatatggag acacctgcgg aaaccgctgg cgcaaagaga tccccgcgta
     5581 ctatgtgata aacgcgaagc tcgcctacac cttcatgaaa aactaccggg cgttcgtgaa
     5641 cctgtataat atcaccaacc agcggtacac ggccatggtg gtgaaggcaa acgttgggac
     5701 cgacgcatca ccgaattacc gcggtttcga ctatcccatg ccgggctttc acttcctggc
     5761 gggactttcg gccaccttct gactcataga ccgactcaca cgctcgcggc ggggcgcggt
     5821 ccgttcgatg cgctccgccg tcctacttgc cgatacgctt tttgatccac gagtccacgt
     5881 cgaagcgccc ggcctcgctg ccgtagagga tcgcgttcag gatctcccgt tcgaactccg
     5941 tgttgttcac cgccgcctcg accgtgcgcg catcataccc gtgcttcttc gcaagataga
     6001 gcgcgcaacc ccgcttgaac gattccttga cgctcgcgaa cgaaccgccg ccgcgcatca
     6061 cgatatttac gatcccggcg tcggtcgcga cgatctcgct cacgagcggg tcgcggatga
     6121 actcgcgcat gcgcgcgttc atgatccggc gcagcgccgc cgggtccttg agcgttttgt
     6181 cgaagatcgg ccggtccggc gaaaccgcgc ccgccgcgac gtatttgccc atcgcggcca
     6241 tccgccgcat gccgtcgagc gtcttgtagg cgtagtcctt cacgtacgag ttgaccacga
     6301 aggccggcat ccccacccgc ggatcggtcc ggagcgtgta cacgacccgg gtcttctccc
     6361 gcccgatgta ttcgagaagg tactggccgg cgaaggtcgt gatgcgcacg aagcgcgtat
     6421 cggccgggac ggcggaatcg gcgagcgccc gcagcttcac gatgccccgc gccgtcgcga
     6481 ggtcgtagac ggtcttggcc tccaccacga tgtctcggtc cgacaccggg gcgggcatgg
     6541 aaagcacgat gcgcagcgtc atgttgttcc ggtcgatctt cgtgacgatc gacgcactcg
     6601 tgcagttcgc catccactgc ggaaaggcgg ccggatcgcg cagcacctcg cccagcacct
     6661 cgagcttcgc gtcgatcacg acgatgccgc ggtattcctt gaaggggaac ttcgcgggct
     6721 ggcgcgagta cacgcggatg ccgtttgtgt tgagctcggt cgtccagcac agggccgggg
     6781 ccgccgtaat gagcgtagcg atgagggctg cgattccgag gagcgccgtg atctcgattc
     6841 gtttcatgat ttctcctgtc gtgcggtttc tggcggtttg tcaatccata tggataatga
     6901 ttgcatattg agcgggattg cggttaagct atgatgaaat atcatattga tattttcgat
     6961 ttatcttaaa ttgacacgca ttcatgtatc ccacatgaaa tccaacccat taaatatttg
     7021 acgaacgtaa cggcaaggaa aaatggagtc attttttcga gggaatgaca tgtcctggtc
     7081 accgaaggtt ttcatcgtcg cagccctgct tgtcgcggtc gccatatacg ccatgttctg
     7141 ctcatcgcgc gacatcagcg gaacgtactc gctcgtcaag tgtgttgatg tggactcgac
     7201 cgagatcggc gaacgcgccg gcgcggcggg gacgatcatc atcaaaaacc tctccggacg
     7261 ccggttcgcg atagagcacg cgggcgggag caacgacggc acgcgcctca tcggcgagct
     7321 ggaggacacc cggctgatcg tcaagatcgg cacctcgccg gtggtctatg ccttcgcctc
     7381 cggatcccgg tcgttcgagt acaacggtta ctccggcagg tgcgtctacg aaaagtcgaa
     7441 ctgagctgcg gcgtcccgcc agatccggcg cagcgcgtcg cgccagcggg gatgcgtgaa
     7501 ggcgcgcccg cgccattcgg cgccctcgat caccagcgtc tccagcaccg cccacgcgcg
     7561 cgcgaaatcg aactcgcgcc agagcgtccc cgccttgtag atgaactccg cctcgcgcgc
     7621 gcgcaggagc cgctcgaatc cctcgcggta atcgaacacc ggccagtgcg cgccggcgta
     7681 gaagatgaac tcggcgctgc ccagcgccac gagcgcatcg aagcctttct cgtagtcgaa
     7741 gcgcttccat tcctttccgg cgtagtacag atagcgcggc aacccgagcg cggagaagag
     7801 cgcgatcgcc ccggcgtggt cgaactcgcg ccagaagcgc ccggcgcggt agacgtattc
     7861 ggggtcggcc aggtcgaaga gggcgcggca gccgcgcgcg taatcgaacc gcttccagaa
     7921 gatgcccgcc ttgtggatga actcggccga gccaagcgcg atcagccggt cgaggccgcg
     7981 cgcgtaatcg aactcccgcc agtcgcgccc ggcgaggaac acgcactccg cattgtccgc
     8041 ggcgaggagt gcggggacgc cgcgggcgaa atcgaaatca gcctggtccg cgcccgctcg
     8101 gtacaatgcg cgcgcctcgc tcgatgcgtc atgcccggcg gtcatcgtcg cccccgcttc
     8161 gcgcggatac gcaccgcctg cccgaacggc gcgacgaacg gctcgtcgcg cccctcggcg
     8221 atgagccaga tggtgggcga gtactcgcgc agctcggcga tatcgtagat gtagccgtcg
     8281 gtgatcacca gcagcatctc ggtgtgccct gcgagatacg aattgaacag cggcgcgaag
     8341 aaggtggttc cgccggcgcc ggtgcgtatg aagcgccaat cgcccttttg atagcgatac
     8401 gggcggcccg cgtcgcgcgc ggccacgaag gcgtcgtcgc gcttctcggg aacgaacagg
     8461 ttctcgtcca ggcagaggag gttcacgcgg tatttttcgg caagctgctc gatcacgccg
     8521 aaggccgttt cgatgtcggc cgggttcatc accatggagc ccgagacgtc aacggccacg
     8581 gtgatcacct cgcgctcgcg aaagatgcgg ccgggcgcgt agatgccgtt ggcgaaatag
     8641 cggcggttga agcgcccgta ggtgtaggtc cactcgttcg attgcgcggc gaagtcgatc
     8701 gtgctcttga gcaggcgttc ccacgagtcg atgtcggcct cggttgtcgt gttgatgatg
     8761 ccccggatct cctggaaagc gcgctcgtcg gcgcaatcgc ggtcggcctc ggccaggctc
     8821 agcgtgcgtg tacgcttcgc ggtgatggcg tcggcggccg cgcggtcgtg gaagaggtgc
     8881 acgcccgtgg gcagcgtctc gtcgcgctcg tcgatgaaga cgatcccccg caggccggac
     8941 caatcgaagc ggctgcgctc gtcgtccggc gcgcgaaaca gcggcgcggt gtcgatgcgt
     9001 tcatccaggc gcgcgccatc atgcgccccg gcatccagcg gaatgccggg atctatgggc
     9061 cccgagtcga tatgcgcgcg gcgctcctcg ggcgagagcc ccttgagcca gcggaacacg
     9121 tcctcccacg aggggtcgcc gatccccgtt gcgcgataga acgcgtgcgg aatcaccggc
     9181 aagcccggcg ggaggaggag ctccggcgtg tcggccgcgt aatcgcccgt ccgtgaaaaa
     9241 aaggtcgtgt gatgggcgac gatatagctg ttcaccgcca tgtcctgcgc cacgttctgg
     9301 aggtaggggc tctcgtcggg cagcgcgcgg tgatcgtggt tgagcacggc gtgcatcatc
     9361 tcgtggatca gcaaaccgat gaactgttcc gcgtgcagtg attcgacgaa gtccggacga
     9421 taggccagcg tgaggcgcga ctgcaggacc gtgagcgcga gcgtcggtat cgcggcatcg
     9481 gggaaaaact ggacctgctg atagaaatag gaagcgaacc ggcttcgccg ccacagggtc
     9541 tcgagcgcga tgtcgacggg cgcggacatg atattttagg cttgcgcata tggcgcggcg
     9601 tataatagtg gacggcgagc gccgcgcaca ggccggggcg cgccaatcat gacacaagga
     9661 gctggaccat gtatattacg aaagacggta agaagctcga ggacgttatc cgcaccgcga
     9721 tcgacgatag cgaggtcacc atggccgagt acgaggagat catcaatgtc gcgttcgagg
     9781 acgggaagct cgatgcccat gaaaaggccc tcctgaagga gttcaagtcg ctcatcgcgc
     9841 agaacgtggt caagcgcgtg ccgtaatcca tccgcgggac cctgcgcgat cgcattaccg
     9901 ggtcgctgcg ccggtcccgc ttcccgcaca cggatgcggc gcccgggcgc cgcatcgcaa
     9961 tcaaagaatc cgctcgaacc gttccagcag ggccgcgccc atggccgtat cgggcagccg
    10021 gtgcagcgcg gccggcgcct gcgcgaagat cccgcagtcg cgcaggaggc ggtaaaaaaa
    10081 cgaaatcctg aaatcggcgc gcgcatgccc cgcgaacatg agcacgttcg cgatctcgtc
    10141 gagctcggcg tcggtcggcg tcgcggcgcc gcgcgcccgg gtctcgcgca ggtggtcgat
    10201 cgcgccggag agcgcccaga ggacgtcctc ggcggggatg gcgtcgtcgg ccaactcgcg
    10261 aaggcgcgcg cggtccccgg cgatgacctc cgcggtgacc agccgccgcg gcgcgcagat
    10321 ctgcgccacg aagtcggccg cggcgctcgc gccgatgagc gccgtgatcg tgagcgccag
    10381 cggaccggac ggctccgccg caagccgcgc ggccagggcg tcctcgtcgg caagtccgaa
    10441 ggcgtccgat atcgcgcacg agacctggtg ccagccgcgc gggttggggt tgatggcgcg
    10501 ctcgtcccac agccggtcgg ggaacaccga tatgtatcgc ataacggacg ggtggacgcc
    10561 ctcgcgcgtg gcccagggca gccactcggc ggcggcgggc cggagctgga agaccgcgag
    10621 gcggccgtcg agcgcggcgt cgtcgaagtt ggtgatggtg gtgctgtcct cctcgccgag
    10681 gttgcccgcg agcacgatgg cggtccgttc ggggagcgcg tgctccccgc aggtgcgctc
    10741 ggtgataatc tggaagagcg tctggataac cgtagggtgc gcgcggttgg actcgtcgag
    10801 gaaccacacg atgccggccg cgccgtcggg gacgcgctcc ggcgggatga agcgcggtcg
    10861 gtagtagatc atccgccgcg cctcgtggtc gggcacgggg tagccgccga ggtcgatgtt
    10921 ctccttgaag gccagccgcg tgtcgatgaa gtgcattccc tttcgttcgg cgaccgcctt
    10981 cacgatggcc gatttcccca cgcccgggtg ccccaggacc ttgatcgcat agttgagccg
    11041 ccaccccgcg tcgaagtagc cctcgaggat ctcctcgagc tggcgggccg aaacgatgac
    11101 cggcgtcttt ttcatatgct cttcaggagc aggtccttga ataccttcgc gacctttttc
    11161 atgaaggccg tgacctcctc ctcgcgccgt ccctcgtact tgcggtagtt gaccaggatg
    11221 ccgttcatca tggcgaaaaa gtagtgcgcc aggagccgcg tgtcgccgcg gtagccgagg
    11281 tcgctgatga cctgttcgaa gatgtccagc ccgcgcccca cgagcgcatt gacccgcagc
    11341 agcgagtcgg gattgagctg cccgtgcgtc atgaacacca ccatcatccg aaaataggag
    11401 tcgtgggtgg agatgaagtc gaagaaggtg tcgatgagcc ggtcgaagga atcggcggtc
    11461 acggggccgc ccgcggtgat cttccccagc tcgtcgagaa aggtggtgct gtcgcggacg
    11521 gtggcctcca cgaagagcgc ctcctggttg gggaagtagg tgtagatgga ggaggcggcg
    11581 atcccggcct cgtcggcgat ctcgcgcatg ctcacgcggt tgaacggccg cgtcgcgaac
    11641 acgcgctcgg cggcgtcgat gatgaggtcc ttgcggtctt ggcgctcctt gctcttgagc
    11701 tcgctgagcg tcgacttctt cttggtggtt ttattcacgc ggatagtccc ttggcgttaa
    11761 aagtgtcatc agacaaacgt catttcatat gagcgccata tgtagtgcgg gcgatgattt
    11821 ttatcaagaa aaaatccgaa atctgtttga cagtccgaac ggtgttcggg aaattgatac
    11881 aatctcattc gcacacatca tcgaccagag gtggcccatg attctcctga atccccacaa
    11941 acacaaccgc acgtataaag atgaaaaatc gaaggagctg atgcaaaaga ccatcgactt
    12001 ctttgaaaag aagggattga agcaaatcaa gaaggactgg cacgagaaaa aatggaacta
    12061 tgatttcgtc gagttcctca aggagaccca ggccttcgcc gtgctgatga cgccctccgg
    12121 ctacggcgcc tccgactccc gatgggacac ctatcgcaac agcgccttct cggagatcac
    12181 cggtttctac ggggtcacct actggtacac ctggcaggtg tccatgctgg gcctgtgccc
    12241 gatctggaac ggctcgaacg aggaggtgaa gcacaagacc gcgaagctcc tgcaggaggg
    12301 cggggtgttc gccttcgggc tttccgagaa ggagcacggc gccgacatct actccagcga
    12361 catgatgctc taccccgagg gcgacgggaa gtaccgcgcg aacggcgaca agtactacat
    12421 cgggaacggc aacgaggccg cgctcgtgtc caccttcgcg aagatggccg acaccgggga
    12481 ctatgtcttc ttcgtcgtga actccaagca cccgaagtac gagtgcatcc gcaacgtcgt
    12541 caacgagcag aactacgtgg ccgaatacgc gctgcacgac tatcccatca ccgacgccga
    12601 catcaccgag cgcggcccca aggcgtggga cgacatgctg aacacgatca atgtctgcaa
    12661 gttcaacctc ggctggggcg cgattggcct gtgcacccac tcgctctacg aatcgatcga
    12721 ccatgcggcc aaccgcaacg tctacggcaa gttcgtgacc gactttccgc acgtgaagcg
    12781 cctcttcgtg gacgcctact gcaagctcgt ggcgatgaag ctcttcgccg agcgcgcgat
    12841 cgactacatg cgctccgcca acgagaacga ccgccgctac ctgctgttca atcccatggt
    12901 gaagatgaag gtgacccgcg agggcgagga ggtgatgaac atcctccacg acatcatcgc
    12961 cgccaagggc ttcgagaacg agcccttctt cgagatcgcc aagcacgagc ttcccatgct
    13021 gcccaagctg gaggggacgg tgcacgtgaa catggcgctt atcgtgaagt tcatggccaa
    13081 ctatttcttc aacccgaagg agtatcccga ggttcccaag cgcgacgatc tcgcgaacga
    13141 cgaattcatg ttccgccagg gccccacgaa gggcctgggc aaggtccagt ttcacgacta
    13201 caacatcgcc tacaatagcg tcaacctgcc gaacg
//
LOCUS       DAORHF010000090        13122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3815_length_13122_cov_4.970460, whole genome shotgun sequence.
ACCESSION   DAORHF010000090 DAORHF010000000
VERSION     DAORHF010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13122
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3815_length_13122_cov_4.970460"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKJ16_09140"
     CDS             <1..424
                     /locus_tag="PKJ16_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HNV47194.1"
                     /translation="NTSIAIRKMKRLGRIRTAAILDIDLHFGDGTDTIFKDDPDVRVI
                     NIQSSNPGDFIDETAAALRHLGPRDLIVISAGFDQYEKDWGGNLSTEDYREIGALSAR
                     YAAEHTGGRLCAVLEGGYYIPDLGANAHALIEGMCTRE"
     gene            447..884
                     /locus_tag="PKJ16_09145"
     CDS             447..884
                     /locus_tag="PKJ16_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47195.1"
                     /translation="MRTLPIPFFLLLMFCGMTACRIVETASPPMTVRLAIHAPRVAEY
                     DVYRTAGAGQFERIAPIAPGRFVASIPQMDGGHKEFLCVQYDTHDPMTYGVIRLQRGN
                     TVVRELSLSDIAALPRDANGYSIIAHRPADVPDSISKKNRSRP"
     gene            complement(881..1759)
                     /locus_tag="PKJ16_09150"
     CDS             complement(881..1759)
                     /locus_tag="PKJ16_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020612592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV47196.1"
                     /translation="MGTLRLTFWGVRGSIPVPGQATAKYGGNTSCLELRSDRGDWIIF
                     DAGTGLRTLGDSLDLSKKYSFHIMISHPHWDHIDGFPFFTPVYIPGNSATIYGPATHE
                     FTIADIFGKQMRFTFFPVRTEELVAAIDYRDLSEERLVLGNFTVETIKLNHPVDCLGY
                     KVRYEDKVFVYLGDNEPYYNVYDDGDPEVDATAEAMNRRLADFVAGADALVTDCQYLP
                     AEYEKKRGWGHSTVHHALNMAAGGGVKRVFMFHHDPLRTDAELDYIVEHYRAKIAEKG
                     INLHLFAAAERQSYEF"
     gene            complement(1761..2609)
                     /locus_tag="PKJ16_09155"
     CDS             complement(1761..2609)
                     /locus_tag="PKJ16_09155"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV47197.1"
                     /translation="MKSVHEQVKDSIDRMPSLSPVIHKINEVANNINASAQDLTDVIQ
                     LDPVLTAKVIRMVNSAYFGLPQVVSSLKQAVVMLGVNTIKNVALSSAFLGQVSLKGKT
                     GLDGEDFWRHSLGVAVASKMIAKRLGVEDKLLEEYFIAGLIHDIGKVLVNNFFPEMMD
                     KILALSEKNAGAITDIEKRVIGLTHEEIGIAIGKKWRFENNLLYAMGKHHRPVTSGSA
                     AVFSMVVSVADTFVKILKVGFSGNHRIEPIPEEVWEVLQLNEETAFGTLASIGEEISK
                     AKLFLG"
     gene            complement(2755..3963)
                     /locus_tag="PKJ16_09160"
     CDS             complement(2755..3963)
                     /locus_tag="PKJ16_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47198.1"
                     /translation="MRILMTVIILALCALPAYPFSDQSTAERMLKENKYFIDFLNLCV
                     TNFGAERAGDAKEKFFEIYQLHFNAQVFFLQSDYKNSFRNIRSSQNKGAALYLDILRN
                     VYLEDTKNLLDKLAPDIIKSKNPRARLYLTLAYRDRSISKNFETIGDASNPKLYSYKI
                     FKYIDGLKFSRRAKRYGLLALYESRKDDLKRDIFIHLLEMERESGSKFFNRFLKKSGK
                     TYIDEMDKSYEAHEKEEAAAPKKDEPAKDAKGAPAGTAAKQPGEADKDDAFEKRLEKK
                     VRFRKERSTAQHILQNEFDRAEDIMREYVDDFNFKLIQATLEVLSARKGDGKGEAPAA
                     GDINYAALIKQHYDNYMRTRKDQKTLMDEFASRLKVVDDIDKKGEEPKKDAAQPPKDE
                     TKKSDDKKTR"
     gene            complement(4002..4421)
                     /locus_tag="PKJ16_09165"
     CDS             complement(4002..4421)
                     /locus_tag="PKJ16_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47199.1"
                     /translation="MVRFAIKVALGLLVFFVVFYLIFYAKRYGEAVFVLSNHEILNPA
                     AESGSAQLSFAANDRVYFFVGREGKTINADLAVIEIEIHEGGAYRHYKQITYEIEKSF
                     PRLAAYIPGEYFSRSGRYRVKALLDGKIVSTKEVVIN"
     gene            4792..5784
                     /locus_tag="PKJ16_09170"
     CDS             4792..5784
                     /locus_tag="PKJ16_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47200.1"
                     /translation="MPDETIQSAHVDDASIVIYKGRFRSNSLSLYGLFLIVFNPYREV
                     ETHNAVVSTFSSLYDYEPHTGWERYEEMILDMKWKGTYNNSMTTSLMDQLGKIATDDH
                     LRKQFADYLSDYRYDAIDSSVFDMVNRVVHDKNLVVESGIQELGLAEYQETKDKRYKS
                     SGSATKSGPALEDGAVILSVEPILAPVKGKPIFELKVGDRIMTKITPNSDRANYFIDL
                     LNLRIENHVKPVPAEVIDIKAESRNDPIEILTKIGPGIYGKCVEDEKQVKLRLYDPAI
                     DGPLAKQGTAGKAAAKKSAPEKKDAGGISTTTYVIIGLFVLILVIFIMLIMISF"
     gene            5869..5997
                     /locus_tag="PKJ16_09175"
     CDS             5869..5997
                     /locus_tag="PKJ16_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47201.1"
                     /translation="MFYLLLRMIHDDLSEGRISQDEYLRAIERLARIHGIRKNGIR"
     gene            5998..7323
                     /locus_tag="PKJ16_09180"
     CDS             5998..7323
                     /locus_tag="PKJ16_09180"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HNV47202.1"
                     /translation="MDQDKEYVLKLAHANKVKFIRLWFTDILGFLKSFAITIEELEDA
                     LAEGVRFDGSTLLGFARDDEREMIALPDASTFRILPWRPREDSVARIFCDIHLTDMSP
                     FEGDPRYVLKRNLTKAAEKGYTFYTGPEIEFFFFKNAHSTETLDTGGYFDLTPLDTAS
                     DYRRRAVLILEEMDIGVISSHHEAAYSQHEIDLRHEDALTMADNIMTFKLIAKEIGQL
                     NGIHTSFMPKPITDQNGSGMHIHQSLFAGESNTFFDAAKPNFLSDIGRAYVAGLLRHC
                     KEFFAISNQWVNSYKRLQYGFESPTHISWGLGNNTSLIRVPQCPKHKPNSQRVELRNP
                     DPACNPYLLFSAILASGLKGIEEGYTLPEPFDMKPGTQQRESLGRQGIDPIPESLREA
                     LRHLEGSALMEDTLGPFIKNAFIQNKLMEIEKFNIHVTDFELKEYLPRL"
     gene            7292..8398
                     /locus_tag="PKJ16_09185"
     CDS             7292..8398
                     /locus_tag="PKJ16_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV47203.1"
                     /translation="MSSKSTSPGSSAVALVGCGRIGHLLEDDPLRYKPCTHYGGAAAA
                     GITITHACDVNAGRLARFSERAGIPESNRFAEYRALITDVRPGIVIIATWTDSHAEIG
                     ALAARNGARVIMCEKPIAHDLAAARRLISACARHRVRLVVNHERRYESRYRAVHDMLA
                     NGVIGEVTSAWGCVHTGNYRGASHPGEGGGPLLHDGTHLIDIIRFLLGDIRTVEGAFT
                     RSGRRRGYEDRATAWLTTRSGTDVFIEAGGGRKYFSFELHLSGTEGRIVIGNGYQALY
                     RARPSRLYRGFRDLVETPFPAVDGVNCFTRAYREVKRLANDPDAPITSSGRDGYRALE
                     AVHAVYLSASQKGKRVELPVKPGMIDLRAIFKLS"
     gene            8403..8753
                     /locus_tag="PKJ16_09190"
     CDS             8403..8753
                     /locus_tag="PKJ16_09190"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47204.1"
                     /translation="MEKMEIKTTRPWDRTAMLRLSGKFNIEQVVPFEEAVGDALQDDA
                     TALGIDMSHVEYVDSSAIGCLIKYANVTKGKGVDLYLLDLKPDIRLIFKTAYLDKFFN
                     FITHEEFKRRRKDA"
     gene            complement(8750..9403)
                     /locus_tag="PKJ16_09195"
     CDS             complement(8750..9403)
                     /locus_tag="PKJ16_09195"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005048089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HNV47205.1"
                     /translation="MFTGLIEEIGSVARVETAGSGLVMAVSAKAVCAGMRVGDSISID
                     GACQTVTSCTESVFTVFASSVTAGVTTLGSFRPGRRVNLERALTLSSRLGGHIVQGHV
                     DGTATIRAVERDVSGLRITLAVPAGLMRYIAAKGSIAVDGISLTVVSVEDSSVSLYLI
                     PETIGATTLTEKRAGDAVNVETDILAKYVERLLSAGGSGADDADRRLMGKLFEGGFL"
     gene            complement(9403..10644)
                     /gene="ribD"
                     /locus_tag="PKJ16_09200"
     CDS             complement(9403..10644)
                     /gene="ribD"
                     /locus_tag="PKJ16_09200"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HNV47206.1"
                     /translation="MPRNCSEREKRYMRLACEAAFGAIGRTSPNPPVGALLVRGDAIV
                     STGATMACGLDHAEICAIRQADARDLNGAEMFVTLEPCCHHGKTPPCVDAIIEAGIAR
                     VYLPLCDPNPRVAGKGVAALRAAGVDVVMMPEFAPAAFDLIRPFAKYIQTGRAHVMHK
                     SAMTLDGRTATHSGNSQWISSEHSRFIVHLLRGMVDAIVIGCGTLVADNPTLNARPDS
                     FSREIAAYFSSGSHRRSGRDDVVLDMTLSARGSRERRSPLRIIVGLPERIDPAFNIFF
                     DDRFLFYAEPHDLARVSSRSDTEIVRKLADAGCIVTMRGETTAERARFITTDLAARGI
                     IYAMLEGGARTAGAFFDAGEIDEFLYIVAPKVLGDGLPTIAGTGPNAIAEALALHEVS
                     CVFAGGDVVYHAYREPFRGGE"
     gene            complement(10634..11539)
                     /locus_tag="PKJ16_09205"
     CDS             complement(10634..11539)
                     /locus_tag="PKJ16_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47207.1"
                     /translation="MAILPCVIALAVSGAVHSAPQRPAKVPPEYRPDAILGFTKHLIA
                     SGEYYRAYGEIARLRALHPGAVDPARLHATELYLLFMGERYSALRQMRPPAGRDIGVM
                     DALFRVDSYFAQRDYSAVDALLRREKSIYGLDAGTDESFVKRLLAADIMLARRDAMRE
                     IADERLTAINGVDVSKYRELMQYSGERLDALKSPAAAVAYGIIPGMGYVYADRTGTGV
                     LTLVVVTVFSTLTYFAFNTHNEHIGVFTGAIAGFFYGGSILGGYMETKKYNRELMESL
                     LGTVSDELRFEYDRNELYRRHGIAP"
     gene            complement(11570..12469)
                     /gene="secF"
                     /locus_tag="PKJ16_09210"
     CDS             complement(11570..12469)
                     /gene="secF"
                     /locus_tag="PKJ16_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HNV47208.1"
                     /translation="MKTTKIIPFQKYRYFAIGLSALLLVIFSAGTVLHGGFTWGVDFA
                     GGVKLIAAFNKDVSMDKIRDSLARRSINAMVQQIGPEEKNEYIIATKLLEKGESSEKS
                     LETIKKALTDDFKTITILSVETVGPAIGNYLKREAVKLFVLSLIMMMVYLTFRFEFKF
                     GLGALLALIHDVALSVAFCGFFKIEMNIPIIAAVLTIFGYSGNDTIVIFDRIRETLGD
                     KTKLSIADVVDVSVTHTLSRTILTSLLTLFAVVSLYLLGGDVIRDFALVLMFGIFIGT
                     YSSIYIASPIVVWWEKVIRKKAV"
     gene            complement(12480..>13122)
                     /locus_tag="PKJ16_09215"
     CDS             complement(12480..>13122)
                     /locus_tag="PKJ16_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecD/SecF family protein translocase subunit"
                     /protein_id="HNV47209.1"
                     /translation="ITSGQALIQGDFSQEEVEMLTRIIKEGALPVDLRIMEERSVGPS
                     LGQDSINDGLKALLVATGGIGLFMILYYKFAGLISGLGLVLNSIFLLALLSRFGFVIT
                     LPGIAGFILTVGMAVDANVLIYERMREEQRSGKSPASVVTLAFDRAFWTIFDSNLTTM
                     IGAIFLSRVPGSIKGFSVTLIIGLICSMFVACYVTRFVYELIILKKDLKKISV"
BASE COUNT         2699 a         4151 c         3821 g         2451 t
ORIGIN      
        1 caacacctcg atcgcgatac ggaagatgaa acggctgggg cgcatccgca ccgccgccat
       61 actcgacatc gatctgcact tcggggacgg caccgacacg atcttcaagg acgatccgga
      121 cgtgcgggtc atcaacatcc aatcgtccaa tcccggcgat ttcatcgacg agaccgccgc
      181 ggccctgcgc catctcggcc cgcgcgacct gatcgtcata tcggcggggt tcgaccagta
      241 cgagaaggac tggggcggca acctttccac ggaagactat cgcgaaatcg gcgcgctgag
      301 cgcgcgctat gccgccgaac acacgggcgg acgattatgc gccgtccttg agggcgggta
      361 ctacatcccc gacctcggtg cgaacgcaca tgcgcttatt gagggaatgt gcacgaggga
      421 atagcgacat gggaacgaat aacacaatga gaacgctgcc gattccgttt ttccttttgc
      481 tcatgttctg cggcatgacc gcctgccgca ttgtggaaac ggcctcgccg ccgatgacgg
      541 tccgcctggc gatacacgcg ccgcgtgtcg cggaatatga cgtatatcgc accgcaggcg
      601 ccggtcaatt cgagaggatc gcgccgatcg cgccgggccg cttcgtagcg agcatcccgc
      661 agatggacgg cggccacaag gaattcctgt gcgtacaata cgacacccac gatccgatga
      721 cctatggcgt cattcgcctc cagcgcggga acaccgttgt gcgcgagctg tcgctcagtg
      781 acatcgctgc gctgccgcgc gacgccaacg gctattcgat catcgcccat aggccggctg
      841 acgtgccgga ttcaatatcg aaaaaaaacc ggtcccggcc ctagaactcg tagctctggc
      901 gctcggcagc ggcaaagagg tgcaggttga tccccttctc ggcgatcttc gcgcgatagt
      961 gctcgacgat atagtcgagc tcggcgtcgg tgcgcagtgg gtcgtggtgg aacatgaaca
     1021 cgcgcttcac cccgccgccc gcggccatat tgagggcgtg atgcaccgtg ctgtgccccc
     1081 acccgcgctt tttctcgtac tcggcgggca gatactggca gtccgtgacc agcgcgtcgg
     1141 ccccggcgac gaaatcggcc aggcggcggt tcatcgcctc cgcggtcgca tcgacctcgg
     1201 gatcgccgtc gtcatagacg ttgtagtacg gctcgttgtc ccccaggtac acgaacacct
     1261 tgtcctcgta gcggaccttg tagcccaggc aatcgaccgg atggttgagc ttgatggtct
     1321 cgaccgtgaa attgcccagg accaggcgct cctccgagag gtcgcggtag tcgatcgccg
     1381 cgacgagctc ttccgtgcgc acggggaaaa aggtgaagcg catctgcttg ccgaagatgt
     1441 ccgcgatggt gaactcgtgg gtcgcggggc cgtagatcgt ggcgctgttt ccggggatgt
     1501 acaccggcgt gaaaaacgga aagccgtcga tgtggtccca gtgcgggtgc gagatcatga
     1561 tatggaagga atactttttc gacaggtcca gactgtcgcc taacgtccgc aggccggttc
     1621 ccgcgtcgaa gatgatccag tcgccgcggt cggaccggag ctcgagacag gaggtgttcc
     1681 ccccgtactt cgcggtcgcc tgcccgggaa ccgggatcga cccgcgaacg ccccagaatg
     1741 taagccgcag cgtgcccatt ctatcccaga aagagcttcg ccttgctgat ctcctcgccg
     1801 atgctggcga gcgtgccgaa ggccgtctcc tcgttgagct ggagcacttc ccacacctcc
     1861 tcggggatcg gctcgatgcg gtgatttccg ctgaacccca ccttgaggat cttcacgaag
     1921 gtgtcggcga ccgacaccac catcgagaac accgccgcgg acccgctggt gaccgggcga
     1981 tgatgcttcc ccatggcgta caggagattg ttctcgaagc gccatttctt gccgatcgcg
     2041 atgccgatct cctcgtgcgt gaggccgatc acgcgcttct cgatgtcggt gatcgcgccc
     2101 gcgtttttct ccgagagcgc gagaatcttg tccatcatct ccggaaagaa attattgacc
     2161 agcaccttgc cgatgtcgtg gatcagcccc gcgatgaagt actcctcgag cagcttgtcc
     2221 tcgaccccga ggcgcttggc gatcatcttc gaggcgacgg ccacgccgag cgagtgccgc
     2281 cagaagtcct cgccgtcgag cccggtcttg cccttgaggc tcacctggcc caggaaggcc
     2341 gacgagagcg cgacgttctt gatggtgttc acgcccagca tcaccaccgc ctgcttgagg
     2401 gagctgacca cctgcgggag gccgaaatac gccgagttga ccatgcgaat caccttcgcg
     2461 gtgagcaccg gatcgagctg gatgacgtcg gtgaggtcct gcgcggaggc gttgatattg
     2521 ttggcgacct cgttgatctt gtggatcacc ggggaaaggc tgggcattcg gtcgatggag
     2581 tccttgacct gctcgtgaac gctcttcatg acacccgcgc tagttggcac gccagtacac
     2641 gaaatcgatg agaatcatgg cccagaaaaa cgccgtgtag aacacggcaa gggcgaagag
     2701 gatcttctgt aacctgtgca taacaccccg tcggcgcgat gggcccggta tagcctaccg
     2761 ggtttttttg tcatcactct tttttgtttc gtccttcggc ggctgcgcgg catccttctt
     2821 cggctcctcg cccttcttgt cgatatcgtc gacgaccttg agccggctcg cgaattcgtc
     2881 catgagggtc ttctggtcct tccgcgtgcg catgtaattg tcgtagtgct gcttgatgag
     2941 cgcggcgtaa ttaatgtcgc ccgccgcggg ggcctcgccc ttcccgtccc ccttgcgcgc
     3001 ggagagcacc tcaagcgtcg cctggatcag cttgaagttg aaatcgtcca cgtactcgcg
     3061 catgatatcc tcggcgcggt cgaactcgtt ctggagaatg tgctgcgcgg tgctgcgctc
     3121 cttgcggaac cggaccttct tctcgagacg cttttcgaag gcatcatcct tgtcggcctc
     3181 gcccggttgt ttcgcggcgg tcccggcggg cgcgcccttt gcgtccttcg ccggctcgtc
     3241 cttcttgggt gcggccgcct cctccttctc gtgggcctcg taggacttgt ccatctcgtc
     3301 gatataggtc ttgcccgatt tcttcaaaaa gcggttaaag aacttgctcc ccgactcgcg
     3361 ctccatctcc agcaggtgga tgaagatatc gcgcttcagg tcgtctttgc ggctttcata
     3421 caacgccagc aggccgtagc gtttcgcccg gcgcgagaac ttgaggccgt caatatactt
     3481 gaagatcttg tacgaataca gcttcgggtt cgacgcgtcg ccgatggtct cgaagttttt
     3541 actgatggag cggtcgcggt aggccagcgt gagatacagc cgcgcgcgcg ggttcttcga
     3601 tttgatgatg tccggggcca gcttgtccag gaggttcttg gtgtcctcga gatagacgtt
     3661 ccgcaggatg tccagataca gcgcggcccc tttgttctga gaggagcgga tattgcgaaa
     3721 cgagttcttg tagtcggact gaaggaagaa tacctgcgcg ttgaaatgga gctggtagat
     3781 ctcgaaaaac ttctccttgg cgtccccggc ccgttccgcg ccaaagttgg tgacgcagag
     3841 attcagaaaa tcaatgaagt atttgttctc cttgagcatc cgctcggccg tgctctggtc
     3901 ggaaaaggga tacgccggga gcgcgcacag cgcaaggatg ataacggtca tgagtatgcg
     3961 cacgggattc acctcatcaa gtattcaacg ccacggtccg ctcagtttat gacgacctct
     4021 ttggtcgata cgattttacc atccagaagc gccttcaccc gataccgtcc cgagcgggag
     4081 aaatactccc ccgggatgta ggccgcaagg cgcgggaacg acttctcaat ctcataggtg
     4141 atctgcttgt aatgccgata cgcgccgccc tcgtgtatct cgatctcgat cacggccaaa
     4201 tcggcattga tcgtctttcc ctccctgccg acaaaaaagt acacccggtc gttcgcggcg
     4261 aagctgagtt gcgcgctgcc ggactccgcc gccgggttga gaatctcatg gttcgaaagg
     4321 acaaaaaccg cctccccata gcgcttcgca tagaagatca gatagaacac cacgaagaag
     4381 accagcaggc ccagcgcgac cttgatggca aaccgcacca ttccccgatc cgggcctctc
     4441 agcgacccga agaatccgcc ccgtctgctg cgaccggaaa atgtctcaac tctattgtcg
     4501 tcattttccc gtcccatgat aacccctccg catccccggt tgatatgaat cgccgttttc
     4561 accgacccgg ccgagataaa ggcttccgga ttcaatccaa aaagtcaaca atttcaatta
     4621 tttagtaaaa aaaatcgcac cgtaggcacg gcaaatattt gacctggata tgttttttat
     4681 attgacacaa agacgccgga cgttagtctt ttaatggaca agctgatatt gtcacgacaa
     4741 tcctaaacag acaacaagac cgattgcaac cgtaaaaaac gatacccgtt catgcctgac
     4801 gaaaccattc aaagcgcaca tgtggacgac gcctcgattg taatctacaa ggggcgcttt
     4861 cggtccaact ccctgagcct ctacggactc ttcctcatcg tgttcaatcc ctaccgcgag
     4921 gtggaaacgc acaatgcggt cgtgtccacc ttctcgtcgc tctacgacta cgagccccat
     4981 accggatggg agcggtacga ggagatgatc ctcgacatga aatggaaggg gacctataac
     5041 aacagcatga ccacctccct catggaccag ctggggaaaa ttgcaaccga cgaccacctg
     5101 cgaaaacagt tcgccgatta cctgagcgac taccgctacg acgccatcga ctcgtcggtc
     5161 ttcgacatgg tcaaccgcgt ggtgcacgac aagaacctcg tcgtcgaatc aggcattcag
     5221 gagctcgggc tcgccgaata ccaggagacc aaggacaagc gctacaagtc atccggcagc
     5281 gcgaccaaat cgggtccggc cctcgaggac ggcgccgtca tcctctccgt ggagcccatc
     5341 ctcgcccccg tgaagggcaa gcccatcttc gaactgaagg tcggcgaccg catcatgacc
     5401 aaaatcacgc ccaactcgga ccgggcgaac tacttcatcg acctgctcaa tttgcgcatc
     5461 gagaaccacg tcaagccggt cccggccgag gtcatcgaca tcaaggccga gagccgcaat
     5521 gaccccatcg agatcctcac gaagatcggg ccggggatct acggcaagtg cgtcgaggac
     5581 gaaaagcagg tgaagctgcg cctgtacgat cccgccatcg acgggcccct cgccaaacag
     5641 ggcaccgcgg gcaaggccgc agcgaagaaa tcggccccgg aaaaaaagga cgccgggggc
     5701 atttccacga ccacgtatgt gatcatcggg ctgttcgtac tcatcctggt cattttcatc
     5761 atgctcatta tgataagctt ctgagttttg gggccgtttc cgcctcatgt gatcgcgcaa
     5821 acctgccgat agggtaaggg ggaaaaacca actgtcggga ggtgaggtat gttctatctc
     5881 cttctgcgta tgattcacga cgatctgtcg gaagggagga tctcgcagga cgaatacctg
     5941 cgggcgatcg aacggctggc ccgaatacac ggtatcagga aaaacggaat caggtaaatg
     6001 gaccaggaca aggaatacgt actcaagctc gctcacgcga acaaggtgaa gtttatccgc
     6061 ttgtggttca ccgacattct cggcttcctc aagagcttcg ccatcaccat cgaggagctc
     6121 gaggacgccc tggcggaggg ggtccgcttc gacgggtcca ccctgctcgg gttcgcgcgc
     6181 gacgacgagc gggagatgat cgccttgccc gacgcatcga cattccgcat actgccctgg
     6241 cgcccgcggg aggactcggt cgcgcgcatc ttctgcgaca tccacctgac cgacatgtcc
     6301 cccttcgagg gcgatccgcg ctatgtgctc aagcgcaatc tcacgaaggc cgccgaaaag
     6361 ggctacacct tctataccgg accggagatc gagttcttct ttttcaaaaa cgcccattcc
     6421 accgagaccc tggacacggg cggctatttc gaccttacgc cgctcgacac cgccagcgac
     6481 taccggcgcc gcgcggtgct catcctcgag gagatggaca tcggcgtcat ctcgtcccat
     6541 cacgaggccg cctacagtca gcacgagatc gacctgcggc acgaggacgc cctcaccatg
     6601 gccgacaaca tcatgacctt caagctcatc gccaaggaga tcggccagct caacgggatc
     6661 cacacctcgt tcatgcccaa gcccatcacc gaccagaacg gctcgggcat gcacatccac
     6721 cagtcgctct tcgccggcga gtcgaacacc ttcttcgatg cggccaagcc gaacttcctg
     6781 tccgacatcg ggcgcgccta tgtcgcgggc ctgctcaggc actgcaagga gttcttcgcc
     6841 atcagcaacc agtgggtcaa ctcgtacaag cggctgcaat acggcttcga gtcgcccacc
     6901 cacatctcct ggggcctggg caacaacacg tcgctcattc gcgttccgca atgtccgaag
     6961 cacaagccca attctcagcg cgtcgagctg cgcaatcccg atcccgcatg caacccgtac
     7021 ctgctcttct cggcgatcct cgccagcggc ctcaagggga tcgaggaggg ctataccctg
     7081 cccgaaccct tcgacatgaa accgggaacg cagcaacggg aaagcctcgg gcggcagggg
     7141 atcgatccca tccctgaaag cctgcgggag gcccttcggc acctcgaggg cagcgcgctc
     7201 atggaagata cgctgggccc cttcatcaag aatgccttta tccagaacaa gctcatggaa
     7261 atcgagaagt tcaacatcca cgtgaccgat tttgagctca aagagtacct ccccaggctc
     7321 tagcgccgtc gcgcttgtcg gttgcggtcg gatcggccat ctcctcgagg atgatccgct
     7381 ccgctacaag ccgtgcacgc attacggcgg cgccgccgcg gcgggcatca ccatcaccca
     7441 tgcctgcgac gtgaacgccg ggcggctcgc ccgtttcagc gagcgcgcgg ggattcccga
     7501 atcgaaccgc ttcgccgagt accgcgccct catcacggac gtccggcccg gaatcgtcat
     7561 catcgccacc tggacggact cgcacgccga gatcggcgct ctcgccgctc gaaacggagc
     7621 gcgcgtcatc atgtgcgaaa aaccaatcgc ccacgacctt gccgcggcgc ggcgtctcat
     7681 ctccgcgtgc gcgcgccatc gcgtccggct cgtcgtgaac cacgagcgcc gctacgaaag
     7741 ccgctatcgc gccgttcacg acatgctcgc taacggcgtg atcggcgagg tcacgtcggc
     7801 atgggggtgc gtgcacacgg gcaactaccg cggggcatcg catccgggcg aggggggcgg
     7861 tcccctcctc cacgacggca cgcacctcat cgacatcatc cgctttttgc tgggcgatat
     7921 ccgcaccgtc gagggcgcct tcaccaggtc cggcaggcgc cgcggctacg aggaccgcgc
     7981 caccgcatgg ctcacgaccc gctccggaac ggatgtgttc atcgaggcgg gaggcggcag
     8041 gaaatatttt tcctttgaac tgcacctgtc gggcaccgag ggaagaatcg tgatcgggaa
     8101 cggataccag gccctctatc gcgcgcgacc gtcgcgactg taccgcggat ttcgcgacct
     8161 cgtggaaacg ccctttcccg ccgtcgacgg cgtcaactgc ttcacacgcg cctaccgtga
     8221 ggtcaaacgg ctcgcgaatg atccggacgc ccccatcaca tcgagcggac gcgacggcta
     8281 ccgcgcgctc gaggcggtcc acgccgttta cctttcggcg tcgcaaaagg gaaaaagggt
     8341 tgaacttccc gtcaaaccgg gcatgattga ccttcgcgcg attttcaaac tctcatagcg
     8401 acatggaaaa gatggaaatc aagacgacac gtccgtggga ccgcaccgcg atgcttcgcc
     8461 tgtccggtaa gttcaacatc gagcaggtgg ttccgttcga ggaggcggtg ggcgatgcgc
     8521 tgcaggacga tgccacggcg ctcgggatcg acatgtcgca cgttgaatac gtcgattcgt
     8581 cggcgatcgg ctgtctcatc aagtacgcga acgtcaccaa gggcaagggc gtggacctgt
     8641 acctgctcga cctcaagccg gacatccggc tcatcttcaa aaccgcctat ctcgacaagt
     8701 ttttcaattt tatcacgcac gaagagttca agcggcggcg caaggacgcc tagaggaacc
     8761 ccccctcgaa gagctttccc atgaggcgtc gatcggcatc gtccgcaccg gacccgccgg
     8821 cggacagcag gcgctcgacg tatttcgcga ggatatcggt ttcgacgttc acggcgtcgc
     8881 cggcgcgctt ctcggtgagc gtggtcgcgc cgatcgtctc gggaatgaga tagagcgaca
     8941 ccgacgaatc ctcgacggac acgacggtca gcgagatccc gtcgacggcg atcgagccct
     9001 tcgcggcgat gtaccgcatc agcccggccg ggaccgctag cgttatgcgc aatccagaca
     9061 catcccgctc gaccgcgcgt atggtcgcgg tgccgtcgac gtgcccctgc acgatgtgcc
     9121 cgcccaggcg agacgaaagc gtgagcgcgc gctccaggtt gacccgccgt cccggccgga
     9181 aggagcccaa cgtggtcacg cccgccgtga ccgatgaggc gaaaaccgta aagacgctct
     9241 ccgtgcagga ggtcacggtc tggcaggcgc cgtcaatcga gatggaatcg cccacgcgca
     9301 tccccgcgca caccgccttc gccgacacgg ccatgacaag ccccgacccc gccgtttcga
     9361 cccgcgcgac ggatccgatc tcctcgataa gtccggtaaa catcactccc ctcccctgaa
     9421 cggctcgcga taggcatgat acacgacgtc gccgccggcg aatacgcaac tcacctcatg
     9481 gagcgccagc gcctcggcga tggcgttcgg gccggtcccg gcgatcgtcg ggaggccgtc
     9541 gccaagcacc ttcggcgcaa cgatatacag aaactcatcg atctccccgg catcgaaaaa
     9601 tgcccctgcg gttctcgccc ctccttcaag catcgcataa atgatgccgc gcgcggcaag
     9661 gtctgttgtt ataaaacggg cgcgctccgc cgtcgtttca ccgcgcatgg tgacgatgca
     9721 ccccgcatcg gcaagtttac gtacgatttc cgtatcggaa cgcgatgaca cccgcgccag
     9781 atcatgcggc tccgcataga aaaggaagcg atcatcaaaa aatatattga acgcgggatc
     9841 gatccgctcg ggaaggccca cgatgatccg cagcggcgag cgccgctcac ggctccccct
     9901 cgcggagagc gtcatgtcca gtacaacgtc atcacggccc gagcgccgat gcgaaccaga
     9961 cgagaagtac gcggcgatct cccgagaaaa cgagtccggg cgcgcattga gcgtcgggtt
    10021 gtcggccaca agcgtgccgc agccgatcac gatcgcatcg accatccccc gcagcaggtg
    10081 gacgatgaag cgcgaatgct ccgacgagat ccactgcgaa tttccgctgt gcgtggcggt
    10141 gcgcccgtcg agcgtcatcg ccgacttgtg cataacgtgc gcgcgcccgg tttggatgta
    10201 cttcgcgaaa ggacggatca gatcgaaggc ggccggcgcg aactcgggca tcatcaccac
    10261 atcgaccccg gccgcacgaa gcgcggcgac gcccttcccc gcaacccgtg gattgggatc
    10321 gcacagcggc aggtatacgc gcgcgattcc cgcctcgatg atcgcgtcga cgcatggcgg
    10381 ggttttaccg tggtggcagc agggctcgag ggtcacgaac atctcggcgc cgttcaggtc
    10441 ccgcgcgtcc gcctgccgga tggcgcatat ctcggcgtga tcgagcccgc aggccatcgt
    10501 cgcgccggtc gaaacgatcg cgtccccccg cacgaggagc gcaccgacgg gcggattggg
    10561 cgaggtgcgc ccgatggccc cgaatgccgc ctcgcaggcg agtcgcatat agcgcttctc
    10621 gcgttccgaa caattacggg gcaatgccgt gtctcctata gagttcgttc cgatcgtact
    10681 cgaagcgcag ctcgtccgag accgttccga gcagcgactc catgagctcg cgattgtatt
    10741 tcttcgtttc catatatccg ccaaggatgc tcccgccgta aaagaagccg gcaatcgcgc
    10801 cggtgaacac cccgatatgc tcattgtgcg tattgaaggc gaagtaggtg agcgtggaaa
    10861 atacggtcac cacgaccagc gtgaggacgc cggtccccgt gcgatcggca tagacatatc
    10921 ccatcccggg aatgatcccg tacgcgacag ccgccgcggg gctcttgagg gcgtcgagac
    10981 gctctcccga atactgcatg agctcgcggt acttgctcac gtccacgccg ttgatcgccg
    11041 tgagccgctc gtccgcgatc tcgcgcatgg cgtcccggcg ggcgagcatg atgtccgcgg
    11101 cgagcaggcg cttcacgaac gattcgtcgg tcccggcatc aagcccgtag atcgattttt
    11161 cgcgccgaag cagcgcgtcg acggcggagt aatcgcgttg tgcgaagtag gaatcgaccc
    11221 tgaagagcgc gtccatcacg ccgatgtcgc ggccggccgg gggacgcatc tgccggagcg
    11281 ccgaatagcg ctcccccatg aacagcaggt agagctcggt cgcatggagg cgcgccggat
    11341 cgaccgcgcc gggatggagc gcacgcaatc gcgcgatctc cccataggcg cggtagtact
    11401 cgccggacgc gatcaggtgt tttgtaaaac cgaggatggc atcgggccgg tactccggcg
    11461 ggaccttcgc cggacgctgc ggcgcggaat ggaccgcgcc cgatacggcc agggcgatga
    11521 cacatggcag gattgccaaa tatcgtccgg cccggcgcat cgattgcggt cagacggcct
    11581 ttttccggat caccttctcc caccatacca cgatgggcga tgcgatgtag atcgaggaat
    11641 acgtcccgat gaaaataccg aacatgagca ccagcgcgaa gtcgcggatt acatcgcccc
    11701 ccaggaggta cagcgacacg accgcgaaca gcgtaagcag cgaggtgagg atggtccgcg
    11761 aaagggtatg ggtgaccgac acgtccacca cgtcggctat cgaaagcttg gtcttgtcgc
    11821 ccagcgtttc gcggatacgg tcgaaaatta cgatggtgtc gttgcccgaa tatccgaaga
    11881 tggtgagcac cgcggcgata atcggaatgt tcatctcgat cttaaaaaaa ccgcagaagg
    11941 cgaccgacag cgccacgtca tggataagcg cgagcagcgc ccccagcccg aacttgaact
    12001 cgaaacggaa ggtgaggtac accatcatca taatgagcga caatacgaac agcttcaccg
    12061 cctcgcgctt caggtaattc ccgatggcgg gccccacggt ctcgacgctg aggatcgtga
    12121 tggtcttgaa gtcgtccgta agggccttct tgatggtctc gaggcttttc tcggacgatt
    12181 cgcccttctc gaggagcttg gtggcgatga tgtactcgtt cttctcctcg gggccgatct
    12241 gctgcaccat cgcattgatc gagcgccgcg cgagcgagtc gcgtatcttg tccatggaaa
    12301 cgtccttgtt gaaggcggcg atgagcttga cgccgccggc gaaatcgacg ccccaggtga
    12361 acccgccgtg caggacggtc ccggccgaga agatgacaag aagcagcgcc gacagcccga
    12421 tcgcgaaata gcgatatttc tgaaagggaa tgatcttggt agtcttcacg gggcgctcct
    12481 tacacggaaa ttttcttcag gtcttttttc aggatgatta attcgtagac gaaccgggtc
    12541 acatagcagg cgacgaacat gctgcagata aggccgatga tcagggtcac cgaaaacccc
    12601 ttgatggaac cgggcacccg cgagaggaag atggcgccga tcatggtggt gaggttcgag
    12661 tcgaaaatgg tccagaacgc ccgatcgaag gcgagcgtca cgacggatgc cggcgatttc
    12721 ccgcttcgct gctcctcgcg catgcgctcg tagatgagca cgttcgcgtc caccgccata
    12781 ccaacggtga ggatgaaacc cgcgataccc ggcagcgtga tcacaaagcc gaagcgagac
    12841 aaaagggcga ggaggaaaat cgaattgaga acgagcccca gcccggagat gagccccgcg
    12901 aacttatagt acaggatcat gaacaacccg atcccgccgg tcgccaccag gagcgccttg
    12961 agcccgtcat tgatggaatc ctgccccagc gacggtccca ccgagcgctc ctccatgatg
    13021 cgcaggtcaa ccggcagcgc gccctccttg atgatgcgcg tgagcatctc cacctcctcc
    13081 tggctgaaat cgccctgaat gagggcctgc ccggaggtga tg
//
LOCUS       DAORHF010000091        13043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3860_length_13043_cov_6.490299, whole genome shotgun sequence.
ACCESSION   DAORHF010000091 DAORHF010000000
VERSION     DAORHF010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13043
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3860_length_13043_cov_6.490299"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3012)
                     /locus_tag="PKJ16_09220"
     CDS             complement(<1..3012)
                     /locus_tag="PKJ16_09220"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47210.1"
                     /translation="MNLEFGHGTSDLPDAARAGAAAARAALGAIRRHPLSCVFVFASV
                     AYDLTELLGAIVDAAGDGVPVIGASTAGEICDGCRERSVVVAALASPHLSVCAAVGES
                     VSKNWRRAFDDCARSPSISRFLHDTETLSQSLRATGEKAFAIMFSPGNTRYADSHACE
                     LVNEFNNRSGGAYPLIGGGCADDWHMEENYVFLGRRAYRDSMLMAFCTTGLAFGMGSA
                     HGFVPTAKPLIVSRCSGHEIFEFDGTPAIGALSAATGFPISELQGKHFAQTTCIPFGY
                     ATAYDEFGLSVGSYFTPAGGIRTTHCFSEGAPITLMESRPDLLASAGKNAVIRALWSG
                     GIRTPAAALMFSCALRSRIAGHTIESEIARVKSVVPDVPVAGFISFGELAPGADGRIH
                     SNNDTVAALVFGDEVTELFRLAGERTAYEKMLRIQHATAVRLSTAVTVDDAFSALMEG
                     ICDTGEFDSGGAYLFREDERSLELVYHSGLGDAFIAAANRYEGESLNVRKVVAGEPIY
                     HDFVSLETLVYHRAPIYRTEGVTSVAAIPLRHEGAIVGSLNVASHRSRSTSDIVKGVL
                     ETMATTAAGVLFRILAHERLRVSNDNLDMLYNTIADFIFIVGADGFVVDTNAVVERRL
                     GYGADEIARMRAVDFHPAERRDEAAAIIGDMLAGLRETCEIPLKAKDGGLIPVETRVT
                     RGTWNGREAIFGVSRDISERVRIENELRIRNDEYDRLVSTIPIGIFTIAATNDSDPRF
                     VYVSPRWCEINQLTREEALANPSAAFSLIHPDEMENFYTHNVESYENGSFFTWEGRFV
                     INGQVRHMHIESQPSRVRDGTLIWNGIIYDITERVAAQEALRVSEEKYRFITENMNDI
                     VWTMDLSLHTTSVSPSIERVLGFTPEERLSQQIEEQVTPDSLSRIRELFAEEMRREEM
                     GVDPNRSVTIETEFRRKDGTALWMVNNILAIRDQSGAIAGIYGVSRDISERKAAEQRL
                     TASLAEKEALLHEVHHRVKNNLQIIVGLLRMQ"
     gene            complement(3002..3394)
                     /locus_tag="PKJ16_09225"
     CDS             complement(3002..3394)
                     /locus_tag="PKJ16_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47211.1"
                     /translation="MARVLLIYDNEAVAGFLKSILEAAAFDVTHVSTPFDAFREALQR
                     APDAILIDFYYKNVDGLYLIEKLRSTAGLTDVPFAVVSEERDFLLSLDALESGANDVI
                     RYPINEDDFIDRMKKICPAQRTGGANEP"
     gene            complement(3409..4674)
                     /locus_tag="PKJ16_09230"
     CDS             complement(3409..4674)
                     /locus_tag="PKJ16_09230"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV47212.1"
                     /translation="MSEQATIDLARFQELKLSFFTKLSGEKVLTFDIIDQSGAVVFEA
                     GIAIDQNEVVAKSQQGCRFFMPKVVESYGHEIVSRPAIQKLADETREIYSSVRATGAM
                     SYEQFLQSNAHLHSIIDGLRQEDRLGGCLGLLRDMEDFDYYTYAHSANLGVLAMVLAY
                     KLGYDKTRMRNMALAGYLHDIGKLGVQREVLSKAGRLTSDEYNQLVEHTRMGYRILDN
                     ITGPGGQKMVSDVIKGTAIFHHRKYKNMGYPFRPARERNSETFYRALPEEIRMIAICD
                     VYDAITTSTPYRTAEEPRTGLRKILNLSNHYFILDDVLSFIKTLACTLNKGKGVLDRG
                     DFIVLESVRSSRDNPNQKEPLYEFARIMSTNSGRPIEPNVHIFFNASDNKKIRSIEVD
                     MRHDYTRKIVRVITNERLTERLRRHALSA"
     gene            complement(4766..5446)
                     /locus_tag="PKJ16_09235"
     CDS             complement(4766..5446)
                     /locus_tag="PKJ16_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV47213.1"
                     /translation="MNNETGVSIVIVEDHPLMRKGIKKVIEDEAAMRVVGEAVSANDA
                     IAIIAREEPDIVIVDISLDAEASGIDLITTMRSRGIASKSIVLSMHTEAYYVERAIRA
                     GAMGYLSKSDAPASIIDAIMAVMSGKLYVSGRISGDLIRGLLDGAEKADGAPVDLLSN
                     RELMVFEMIGAGYKSLEIAGKLNISVNTVEAHRKKIRAKLGCASSGELTRHAIAWLHA
                     NNRPAPLP"
     gene            complement(5560..7773)
                     /locus_tag="PKJ16_09240"
     CDS             complement(5560..7773)
                     /locus_tag="PKJ16_09240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47214.1"
                     /translation="MNGTVLIVEDEGIIADDIAEILTRENYAVCGIVACGDEAVTAAR
                     TLRPDIVLMDIMLPGELDGVDAAALITRIGIPVVFVTAHSNELLMKRAMDVLPYGYLV
                     KPINRRELLAVVGLSIKRHISERGARASERAGPAGRDPSGAAFAGTSAGIAIDDGDRS
                     RTALLADIIDGLPHLVCRFTDDLLLTYANKAYCAFFGKPIDAAAGARFLDVLPAAERA
                     MLQNHIDLIRQTMTEREYFAEISAEDGSSKWIKWTTRPIIAADGRTTGFQSVGEDITP
                     LKSALERLKAAEEKFARAFHHSPVMGSITRMSDGTFLEINDSYEGITGYSRGELIGRT
                     AVEIGIWSSEAREKALALVRSNGHLRNHEVEITCKSGEARTVLLSIEPILIDREPVSI
                     GMAVDITERKRVEAELRHREDMLTRLIENASGVVALQDLRGNILFAAGSVRCARSPHP
                     AAAHPGSPAFDRLMEAIRPLDSAVLDERTEIVEERPIVHDSGTCYFLVQKYPIRDEHA
                     AITAIGTIARDITDVRTLEKEALMAAAAERQRISHRLHDDLGQRLSYLGFAMESVRKS
                     ITQPTPAQRKDLREISRIITEAIEYAKDTAREMHPVTADTERFIHSLRDFITQNERIY
                     KKAVRVDIACRLEGLGDFICDTLYSILTEGIHNALKHGDGDVTLRIRRANDALEVLLE
                     NPIAPATGGAAAGGGMGLGILRHRAVLIGASLATGVADGRFALSIALPLAGRRAE"
     gene            8060..8830
                     /locus_tag="PKJ16_09245"
     CDS             8060..8830
                     /locus_tag="PKJ16_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47215.1"
                     /translation="MKKTTRCVVSTLISAGLVLVFSATRGGADGGRECDSKRALNGAE
                     KAAVNGVFAVLKGSLPAAPKGWKLQQAPDAAKLPAWIAGAPGCPVFYEFESAFYSMKN
                     AMEMGSKMGSQRMTQIAMEIAAAMQRGDTKRAEELQKEMEALQKGATPAEGVSVTVRY
                     RCNGSSSVTLGSECKPVSIPGAKYAFMCESANQTTVAIYLGRWTSSGGSIALADSQKN
                     AANTSPQAIEIVVFGQSASTVQDMARKINLRSLTAVIK"
     gene            complement(8948..10228)
                     /locus_tag="PKJ16_09250"
     CDS             complement(8948..10228)
                     /locus_tag="PKJ16_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47216.1"
                     /translation="MIQNQKESRLHRVSTGIVLCAPIALALASALYYAADIATDPDRS
                     GTLFVAYSGASLGIAIIAIAYLFIRHRLIRSAERRMSGRLRAIAAGNPADPAAHSGEL
                     RELAAALDETAGAFSGRTALLAETADRLSERAAMITGDTAASLDQVKEQALAIIQVGA
                     SIESIAAGVNGVAELSARQSEELQLLVRLIESLIETAETVARNIEAEVDSTGRVAESA
                     GKGQAELDATAREMLGVIDDTKSIGEVLGVINDISDKINLLSLNASIEAARAGESGRS
                     FAVVAGEIAKLADQTAASVKDIGAILDEKNAVLARNTQAIQQAVAASGEVMRRIQDIS
                     RDVHRMAATVRDQSQLNTIVAGKAQTIKATSEEIDDLTVDQKVAVYDVLDNVNAINAV
                     FKENLKTMNAILARANELTAAVQDFLAIIANRAN"
     gene            complement(10315..12315)
                     /locus_tag="PKJ16_09255"
     CDS             complement(10315..12315)
                     /locus_tag="PKJ16_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral/alkaline non-lysosomal ceramidase
                     N-terminal domain-containing protein"
                     /protein_id="HNV47217.1"
                     /translation="MTTRIARIIIAAGLGLGLYACSAPQVKIDIRNIAPAAPAPSDGL
                     VAGAARVDITPPPGMPLAGYSKQSGAAEGFRTRLYARAIYIKPKTGKPIALVQCDLLS
                     GSLILHHRVAELVAKETGVGADALMIAGTHTHSGPGGYFESNFYNRFAAAAPGFDRDY
                     FDFLAGRIAAAIMEAHTTSAPAKVAAGTMDFTGFSRNRSIEAYRHNATVPEAKKNDIF
                     AAVNPSLHVVRVDCRDELGRFVPRAAFSSYSIHATVIPNSNRLYNADVFAAVTREFER
                     LARERLGAGEDFVHAAANGTHGDNSPNYRDARDFPEARRLGSALAARQFELFSALGAK
                     LSDEAACRAIAREIDLATNRSIGGVSLASEPKVGNALLAGAPDGRTPVVGCLPFFAPG
                     WPRWFFTGGEQGHKRIAAGPLQPIILPKEDFPHLLYLQLIQVGNTVLAPLPFEITTES
                     GRRIAAQCASAFTGAAPVVVAPISVSNGYFGYVTTPEEYARQYYEGGHTLYGPNTQPF
                     LAAHAADLARALINGAPSSLPTSWSFTLEGEPFVPKSGDAIVRRSPQGAPEFEQGARG
                     IEASWRFRWIDAPPRAMAFDRPLVRIESSPDGKTWTPLAVAGTPMDDGGYDLSVRLVT
                     KYAEGHLYEARWHNPARNGGQYRFVILPRTPGGEALRSAAFK"
     gene            complement(12504..>13043)
                     /locus_tag="PKJ16_09260"
     CDS             complement(12504..>13043)
                     /locus_tag="PKJ16_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47218.1"
                     /translation="QQQIELLAACGKELDLLLATCSFDPENEIYLCFGALKQKKFIYG
                     KYTHKRFGAHVYENRYTKQECADKACERDIASFLSERGLTEYSRLITCERGVMRAILL
                     SKEIVSSRKEALADRSMAAVFPAYQDGQKYFCHHDAKRRNFICDCGEELLSLQSEKYR
                     HLVGMDDQKFLESHLPGML"
BASE COUNT         2157 a         4093 c         4253 g         2540 t
ORIGIN      
        1 ttgcatccgc aacaagccga cgatgatctg caggttgttc ttcacccggt ggtgtacctc
       61 gtggaggagc gcctccttct ccgcaaggga ggccgtgagc cgttgctccg cggccttgcg
      121 ctccgaaatg tcccgcgaca cgccgtagat gcccgctatc gcgcccgatt ggtcgcgaat
      181 ggccaggata ttgttgacca tccagagcgc ggttccatcc ttccggcgga attcggtttc
      241 tatcgttacc gatcggttgg ggtcgacccc catctcctcg cggcgcatct cttcggcaaa
      301 cagttcccgt atcctactga gcgaatccgg cgttacctgt tcctcgatct gttgcgagag
      361 gcgctcctcg ggagtgaacc cgagcacccg ctcgatggac gggctcacag aggtcgtgtg
      421 aagcgagagg tccatcgtcc acacgatgtc gttcatgttt tcggtgatga atcggtattt
      481 ctcctcgctc acgcgaagcg cctcctgggc ggcgacccgt tcggtgatgt cgtagatgat
      541 cccgttccaa atgagcgtgc catctcgcac cctggacggt tgagactcaa tatgcatgtg
      601 ccgcacctgg ccgttaatga caaatcgccc ttcccatgtg aaaaaacttc cgttttcata
      661 ggattccacg ttgtgcgtgt agaagttttc catctcgtcc gggtgaatca gggaaaaagc
      721 ggcggacgga ttggcaagcg cttcctcccg ggtgagttgg ttgatctcgc accaccgggg
      781 gctgacatac acgaatcggg ggtcgctgtc gttcgttgct gcgattgtaa agatcccgat
      841 ggggatcgtg gagacgagcc tgtcgtattc atcgttgcgg atgcgcagtt cgttctcgat
      901 ccgcacgcgc tccgatatgt cccgcgacac gccaaagatc gcctcccggc cgttccaggt
      961 tccccgcgtg acccgcgtct cgaccggaat gagtccgccg tctttcgcct tgagcgggat
     1021 ctcgcaggtt tcccgcaggc ccgcgagcat atcgccgatg atggccgccg cttcgtcccg
     1081 tcgctcggcg ggatggaaat cgacggcgcg catccgggcg atctcgtccg cgccgtagcc
     1141 gagccgccgt tcgacgaccg cgttcgtatc gacgacgaat ccgtccgcgc caacgatgaa
     1201 gatgaaatcc gcgatcgtat tatacagcat gtcgaggttg tcgttcgata cgcggagccg
     1261 ctcatgcgcg aggatgcgaa acaggacgcc cgcggccgtc gtcgccatcg tttcgagcac
     1321 gcccttcacg atgtcgctcg tgctgcgcga gcggtgcgac gcgacgttca gggagcccac
     1381 gatcgcgccc tcgtgcctga gcggtatcgc ggcaaccgag gtgacgccct ccgttcgata
     1441 gatcggggcc cggtgataga cgagggtttc gagggaaacg aaatcatggt aaatcggttc
     1501 ccccgcgacg accttccgca cgttgaggct ttctccttcg tagcgattgg cggcggcgat
     1561 gaacgcatcg cccagcccgg agtgatacac cagttcgaga cttcgctcat cctcgcggaa
     1621 caggtaggcc ccgcccgaat cgaactcgcc cgtgtcgcaa atcccctcca tcaatgccga
     1681 gaaggcgtca tcgaccgtaa cggcggtcga aaggcgcacg gcggtggcgt gctggatgcg
     1741 gagcatcttt tcatacgccg tccgctcgcc ggcgaggcga aacagttccg tcacctcgtc
     1801 gccgaagacg agggcggcca cggtgtcgtt attggagtga atgcggccgt cggcgcccgg
     1861 ggccagttcg ccaaagctga tgaatccggc gacggggacg tcgggcacaa cggatttgac
     1921 ccgcgcaatt tctgattcga ttgtatggcc cgcgattctc gacctgaggg cgcatgagaa
     1981 catgagcgcg gccgcgggcg ttctgatgcc tccgctccac agggcgcgga tcacggcgtt
     2041 ctttccggcg ctcgccagca gatcgggccg tgattccatg agggttatcg gcgcgccttc
     2101 gctgaagcag tgcgtcgtgc ggatgccccc ggcgggagtg aagtagctgc ccacgctgag
     2161 gccgaattca tcgtacgcgg tcgcgtaccc gaaggggatg caggtggttt gcgcgaagtg
     2221 cttcccttgt agttcggata tcggaaagcc ggtcgcggcg gaaagggccc cgatggccgg
     2281 cgtgccgtca aattcgaata tctcatggcc ggagcagcgg ctcacgatga gcggcttcgc
     2341 cgtcgggaca aacccgtgag cgctgcccat gccgaaggcg agccccgtcg tgcaaaacgc
     2401 catcagcatg gagtcgcggt acgcccggcg gccgagaaac acgtagttct cctccatgtg
     2461 ccagtcgtcc gcgcaccccc cgccgatgag cggataggcc ccgccggagc gattgttgaa
     2521 ttcgttgacg agctcgcagg cgtgcgaatc ggcgtacctg gtgttgccgg gcgaaaacat
     2581 gatcgcgaag gccttttccc cggtcgcgcg cagggattgg gaaagcgttt ctgtatcgtg
     2641 cagaaaacgc gaaatggacg gcgagcgggc gcagtcgtcg aacgcccggc gccagttttt
     2701 cgataccgac tcccccacgg cggcgcatac ggacaggtgc ggcgatgcga gggcggccac
     2761 gaccacgctg cgctcccggc agccgtcgca gatctccccc gccgtggagg ccccgatgac
     2821 cgggacgccg tcgcccgcgg catcgacaat cgcgcccagc agttccgtca ggtcgtacgc
     2881 gaccgacgcg aacacgaaca cgcacgagag cgggtggcgc cgtatggccc ccagcgccgc
     2941 ccgggcggcc gcggctcccg cgcgggcggc gtccgggagg tcgctggtcc cgtgaccgaa
     3001 ttcaaggttc atttgcgcct cctgtgcgct gcgcggggca tatttttttc atccggtcga
     3061 tgaaatcgtc ctcgtttatc gggtagcgga taacgtcatt tgcgccgctc tcgagcgcgt
     3121 cgagcgaaag caaaaagtcc cgctcctccg atacgaccgc gaaggggacg tcggtcaggc
     3181 cggcggtcga acgaagcttt tcgatgagat acagcccgtc gacgtttttg tagtagaagt
     3241 cgatcagaat cgcgtcgggc gcccgctgga gggcctcgcg aaaggcgtca aagggcgtgg
     3301 acacgtgcgt aacgtcgaac gccgcggcct ccagtatgct tttcaggaac ccggccaccg
     3361 cctcgttgtc gtagatcagg agcactcgcg ccatggtgtt cgcctccgtt atgcgctcag
     3421 ggcgtgccgt cgaaggcgtt ctgtcaggcg ctcgttggtg atgacccgca cgatttttct
     3481 cgtgtagtcg tggcgcatgt ccacctcgat ggagcggatc ttcttgttgt cgctcgcgtt
     3541 gaaaaaaatg tgcacgttcg gctcgatcgg ccgcccgctg tttgtgctca tgatccgggc
     3601 gaactcgtag agcggttctt tctggttcgg attgtctcgt gaagatcgca cggattccag
     3661 cacgatgaaa tcgccgcggt ccagcacccc cttccctttg ttcagtgtgc aggcgagcgt
     3721 tttaatgaag gagaggacgt cgtccagaat gaagtaatgg tttgacaggt tcaggatctt
     3781 ccgcaggccg gtgcggggct cctcggcggt gcgatagggc gtggaggtcg tgatcgcgtc
     3841 gtacacgtcg cagatcgcga tcatgcggat ctcctcgggg agggcccggt agaatgtttc
     3901 gctgttgcgc tcgcgcgcgg gacggaaggg ataccccatg ttcttgtact tgcgatgatg
     3961 aaatatcgcg gtgcccttga tgacgtcgct caccatcttc tgtcccccgg gcccggtgat
     4021 attgtcgaga atgcgatagc ccatccgggt gtgctcgacg agctggttgt attcgtcgct
     4081 ggtcagcctc cccgctttcg acagcacctc ccgctgcacg cccagcttcc cgatgtcgtg
     4141 caaatagccg gccagggcca tgttccgcat gcgggtcttg tcgtatccga gcttgtaggc
     4201 gagcaccatc gccaggacgc ccagattggc cgaatgggcg taggtgtagt agtcgaaatc
     4261 ctccatatcg cggagaagcc ccaggcagcc gccgagacgg tcctcctggc gcagcccgtc
     4321 gatgatcgaa tgcagatgcg cgttcgactg caggaactgc tcgtaggaca tggcgcccgt
     4381 ggcgcgcacc gaggagtaaa tctcgcgcgt ttcgtcggcg agcttttgaa tcgccggccg
     4441 ggacacgatc tcatggccgt aggattcgac caccttgggc atgaaaaacc ggcacccctg
     4501 ctgggacttc gcgaccacct cgttctggtc gatcgcgatc ccggcctcga agacgaccgc
     4561 gccgctctgg tcgatgatgt cgaaggtgag gaccttttcg ccgcttagct tcgtgaaaaa
     4621 cgagagcttg agctcctgga agcgggcgag gtctatcgtt gcctgttcgc tcatggggtg
     4681 tccctgccgt gcgcgcgcgc cggcgcgtat tcccggcgag agtacacgca aacgcccggg
     4741 agcgcaatca ccggaaatgg ttattttacg gaagcggcgc ggggcgattg ttcgcgtgga
     4801 gccaggcgat cgcgtggcgg gtcagctcgc cggagctggc gcagccgagc ttcgcccgga
     4861 tttttttccg gtgcgcctcg accgtgttca cgctgatgtt gagcttgccg gcaatttcga
     4921 gggatttgta acccgcgccg atcatctcaa aaaccatgag ctcgcggttc gaaaggaggt
     4981 ccaccggcgc cccatcggcc ttttcggcgc cgtcgagaag gcccctgatg agatcgcccg
     5041 atatgcggcc gctcacgtac agcttcccgc tcatgacggc catgatcgcg tcaatgatcg
     5101 acgcgggcgc gtcgctcttg gacaggtatc ccatggcgcc ggcccgaatg gcccgctcga
     5161 cataataggc ctcggtgtgc atggacagga cgatcgattt cgacgcgatg ccccgcgagc
     5221 gcatggtggt gatgaggtcg attcccgagg cctccgcgtc gagggaaatg tccacgatga
     5281 cgatgtccgg ttcctcgcgc gcgatgatcg cgatcgcgtc gttcgcggac accgcttcgc
     5341 ccaccacgcg catggcggct tcgtcctcga tcaccttctt gatgcccttc cgcatcaggg
     5401 gatggtcctc gacgatgacg atcgatacgc ccgtttcgtt gttcatggca gcgcttcctt
     5461 ccggggtctc gtgccgattt tttcggtgat gccgcaggca tcgaagtctg tgcggaatag
     5521 ttatcgaaac gcatttcgcg tgtcaataac aggatccggc tattccgcgc gccggcccgc
     5581 cagcggaagc gcgattgaaa gcgcaaaccg gccatccgcg acacccgtcg ccagcgacgc
     5641 gccgatcagg accgctcgat gccgcagtat cccgaggccc atcccgccgc ccgccgccgc
     5701 gcctcccgtt gcgggcgcga ttggattctc cagcaggacc tcgagcgcgt cgttcgcgcg
     5761 gcggatccgc agcgtcacgt ccccgtcgcc gtgcttgagg gcgttatgga tgccttccgt
     5821 cagtatcgaa tacagcgtgt cgcagatgaa atcgcccagc ccctcgagtc tgcaggcgat
     5881 gtcgacgcgc accgcctttt tgtatattcg ctcgttctga gtgataaagt ccctgagcga
     5941 atgtataaat cgctcggtgt cggccgttac cgggtgcatt tccctggccg tgtccttcgc
     6001 gtactcgatc gcttcggtga tgatcctcga gatctcgcgc aggtccttcc gttgcgccgg
     6061 cgtcggctgg gtgatggatt ttcgcaccga ttccatggcg aacccgagat acgaaagacg
     6121 ctgcccgagg tcgtcgtgga gcctgtggct gatccgctgc cgctccgcgg cggccgccat
     6181 gagcgcttcc ttctccagag tgcgcacatc ggtgatgtcc cgggcgatcg tcccgatcgc
     6241 ggttatggcg gcgtgttcgt cccggatggg gtatttctgc accagaaagt agcacgtgcc
     6301 cgaatcgtgg acgatcgggc gctcttcgac gatctcggtc ctctcgtcca ggaccgcgct
     6361 gtcgagcggc cgtatcgcct ccatcagccg gtcgaaggcg gggcttcccg gatgcgccgc
     6421 cgccgggtgc ggcgaacggg cgcagcgcac cgatcccgcg gcgaagagga tgttcccgcg
     6481 aaggtcctgg agcgcaacga cccccgaggc gttttcaatc aatcgcgtga gcatgtcttc
     6541 gcgatgccgc agctcggcct caacgcgctt ccgctcggtg atgtcgacgg ccatgccgat
     6601 gctgaccggc tcccggtcga tcagtatcgg ctcaatggac agcagcacgg tcctcgcctc
     6661 gcccgatttg caggttatct cgacttcgtg gttcctgaga tgcccgttgc ttcgtaccag
     6721 cgccagggcc ttttccctgg cctccgagct ccatatgccg atctcgaccg ccgtgcggcc
     6781 gatgagttcg cctctggaat aaccggttat gccttcgtag ctgtcgttga tttcaaggaa
     6841 ggtcccgtcg ctcatccgcg tgatgctgcc catgaccggg ctgtgatgaa acgcgcgcgc
     6901 gaacttctct tcggccgcct tcagccgctc gagcgccgat ttgagcggcg tgatgtcctc
     6961 gccgacggat tgaaacccgg tcgttcggcc gtcggcggcg atgatcggcc gggtggtcca
     7021 ttttatccat ttcgacgatc cgtcctccgc ggagatttca gcgaagtatt cccgttcggt
     7081 catcgtctga cgaatgaggt cgatgtggtt ttgcagcatc gctcgctcgg cggcgggtag
     7141 aacgtcgagg aagcgcgcgc ccgcagcggc atcgatcggt ttcccgaaaa acgcgcaata
     7201 cgctttgttc gcataggtga gcaggaggtc gtcggtaaac cggcacacca ggtggggcag
     7261 tccgtcgatg atgtccgcaa ggagcgcggt tcgagatcga tcgccgtcgt cgatggcgat
     7321 ccccgcgctc gtccccgcga acgccgctcc actgggatcg cgacccgcgg ggcccgcccg
     7381 ttcgctcgcg cgcgctccgc gctccgagat gtgccgcttg atcgacaggc cgacgaccgc
     7441 gagcagttcc cgccggttga tgggcttgac cagatagccg tacggcagaa cgtccatcgc
     7501 gcgtttcatc aggagctcgt tcgagtgcgc cgtgacgaac acgaccggga tgccgatccg
     7561 cgtgatcagc gccgcggcat cgacgccgtc cagctcgccc ggcagcatga tgtccataag
     7621 cacgatgtcc gggcgcagcg ttcgagcggc cgtgacggct tcgtcgccgc acgccacgat
     7681 gccgcatacc gcatagtttt cccgcgtgag aatctcggca atatcgtccg cgattatgcc
     7741 ctcatcctcg acgataagca ccgttccgtt catggctggt ttcccgtttt gttcgtatca
     7801 tcgtgttcct gtggacagat gatcgccgca ccggatactc ctttccggaa acaataccgc
     7861 cgtaaaccat tttttgcgtg tcgcgccttg gcagcgcgct ccgtcacgct ccgaatgcga
     7921 cagagccgaa ccatgtcccc gctgagcaca atcgaccgcc ggccgatgca tttcggattg
     7981 acaatgccgg cgtcggggta tggtttcgcg cttgtagcaa attgccgatg acaatcggcc
     8041 acaaaaaaag gagcgtggta tgaaaaaaac aactcgatgt gtagtttcaa ccctcattag
     8101 tgccggcctg gtgctggtat tttccgcgac gcgcggcggc gccgatggcg ggcgcgagtg
     8161 cgattccaag cgggctctga acggagcgga gaaagccgcc gtcaacggcg tttttgcagt
     8221 gctgaagggt tcgcttcccg ccgcgccgaa gggctggaag ctgcaacagg ccccggacgc
     8281 cgcgaagctt cccgcctgga tcgccggcgc gccgggctgc ccggtgtttt acgagttcga
     8341 aagcgccttc tacagcatga agaacgccat ggagatgggg agcaagatgg gcagccagcg
     8401 catgacgcag atcgcgatgg agatcgccgc cgccatgcag cggggcgaca ccaagcgcgc
     8461 cgaggagctc cagaaggaga tggaggcgct tcagaagggc gccacccccg cggagggcgt
     8521 gagcgtgacg gtccggtatc ggtgcaacgg ctcatcgtcg gtcaccctgg gctccgagtg
     8581 taagccggtg tcgatcccgg gggcgaaata cgcgttcatg tgcgagagcg cgaatcagac
     8641 gaccgtcgcc atctatctcg ggcggtggac ctcgagcggc ggaagtatcg ccctcgcgga
     8701 cagccagaag aacgccgcga acacctcgcc gcaggccatc gagatcgtcg tgttcggaca
     8761 gtcggcctcg acggtccagg acatggcccg gaagataaac cttcggtcgc tcactgccgt
     8821 gataaaatag ccatacggcg ttcgcatgcg acgatacacg gcatccggcg gtttccccgg
     8881 atgccgtgtg cgttatgcga cttatatgcc tccgcggagc gtccttcgcg gtccggcgcc
     8941 ggtgatctca gttggcgcgg ttcgcgatga tggcgaggaa gtcctgcacc gcggcggtga
     9001 gctcgttcgc ccgcgcgagg atggcgttca tggtcttcag gttttccttg aagacggcgt
     9061 tgatggcgtt cacgttgtcc agcacgtcgt acacggccac cttctggtcc accgtgagat
     9121 cgtcgatttc ctccgacgtg gccttgatcg tctgcgcctt cccggcgacg atggtgttga
     9181 gctgggactg gtcgcggacg gtcgcggcca tgcggtgcac gtcgcggctg atgtcctgga
     9241 tccggcgcat cacctcgccc gacgcggcaa ccgcctgctg gatggcctgc gtgttgcgcg
     9301 ccaggactgc gttcttctcg tcgaggatcg cgccgatgtc cttgacgctc gcggcggtct
     9361 ggtcggcgag cttggcgatc tctccggcca ccacggcgaa gctgcgcccc gactccccgg
     9421 cccgcgccgc ctcgatggag gcgttcagcg acagcaggtt gatcttgtcg gagatgtcgt
     9481 tgatgacgcc cagcacctcg ccgatcgact tcgtgtcgtc gatcacgccc agcatctccc
     9541 gcgcggtcgc gtcgagctcc gcctggcctt ttcccgcgct ctcggccacc cgcccggtgg
     9601 aatcgacctc cgcctcgata ttgcgcgcaa cggtctccgc cgtctctatc aggctctcga
     9661 tgaggcgcac caggagctgc agctcctcgg actggcgcgc cgagagctcg gccacgccgt
     9721 tcacgcctgc cgcgatcgac tcgatggacg cccccacctg gatgatcgcg agcgcctgtt
     9781 ccttcacctg gtcgagggag gcggccgtgt ccccggtgat catcgccgca cgctccgaga
     9841 gccggtccgc cgtctcggcg aggagcgcgg tgcggccgct gaacgcgccg gcggtctcgt
     9901 ccagcgcggc ggcgagctcg cggagttcgc ccgaatgagc cgccgggtcc gcgggattcc
     9961 ctgcggcgat cgcgcgcaag cgcccggaca tgcgccgttc cgcggagcgg atcagccggt
    10021 gccgaatgaa aagatatgcg atggcgatga tggcgatgcc gagcgatgcg ccgctatagg
    10081 cgacaaagag cgtccccgag cggtcgggat cggtggcgat atcggccgca tagtagagcg
    10141 ccgaggcgag cgcgagtgca atcggcgcgc agagaacgat gcccgtggaa acgcgatgca
    10201 gccgggattc tttctggttc tgtatcatcc cgtgttcctt ccgattccaa gggtgcgcgg
    10261 gggcgatgaa aatatcaaca caaatacgtt gtgtcgatcc gtaccgcccg tgcgttattt
    10321 gaacgccgcc gaacgaagcg cctcgccgcc cggcgtgcgc gggaggatca cgaatcggta
    10381 ctggccgccg tttcgggccg gattgtgcca ccgcgcctcg tagagatgtc cctcagcgta
    10441 tttcgtaacc agccggaccg agagatcgta cccgccgtcg tccatcggcg tcccggcgac
    10501 ggccagcggc gtccatgttt ttccatcggg gctcgactcg atgcgcacca ggggccggtc
    10561 gaacgccatc gcgcgcgggg gcgcgtcgat ccagcgaaag cgccacgacg cctcgatgcc
    10621 gcgtgcgccc tgctcgaact ccggcgctcc ctgcggcgag cggcgaacga tcgcatcgcc
    10681 cgatttcgga acgaagggtt cgccctcgag cgtgaaggac cacgaggttg ggagtgacga
    10741 cggcgcaccg ttaataagcg ctcgcgcgag gtccgcggca tgcgccgcga ggaacggttg
    10801 cgtgttcggg ccgtagagcg tgtgcccgcc ctcgtagtat tggcgggcgt attcctcggg
    10861 cgtggtcaca tagccgaagt agccgttcga tacgctgatg ggcgcgacga cgaccggcgc
    10921 ggcgcccgtg aaggccgagg cgcactgcgc cgcgatccgc cgccccgatt ccgttgttat
    10981 ctcgaaggga aggggggcga gcacggtgtt tccgacctgg atcaattgca ggtagagaag
    11041 atgcgggaag tcctctttgg ggaggatgat cggctgcagc ggacccgccg cgatgcgctt
    11101 gtgtccctgc tcgccgccgg tgaagaacca gcgcggccac cccggggcga aaaagggcag
    11161 gcagcccacc acgggcgtgc ggccgtccgg ggcccccgcg aggagggcgt tgcccacttt
    11221 gggctcgctc gcgagcgaca cgccgccgat cgagcggttt gtggccaggt cgatctcgcg
    11281 cgcgatggcg cggcaggcgg cttcgtcgga aagcttcgcg ccgagcgcgc taaagagctc
    11341 gaactgacgg gcggcgagcg cgctccccag gcggcgcgcc tcggggaaat cgcgcgcatc
    11401 gcggtagttc ggcgagttgt cgccgtgagt tccgttcgcg gccgcgtgga cgaagtcctc
    11461 gcccgcgccg agccgctcgc gcgccagtcg ctcgaactcg cgcgtcacgg cggcgaagac
    11521 gtcggcgttg tacaggcggt tcgagttcgg gatcaccgtc gcgtggatcg aatagctgga
    11581 aaaggcggcg cgcggcacga agcggcccaa ctcgtcgcgg cagtccacgc ggaccacgtg
    11641 gagcgacggg ttcaccgccg cgaagatgtc gttcttcttc gcttcgggga ccgtcgcgtt
    11701 gtgccgatag gcctcgatgc tgcggttgcg gctgaagccg gtgaagtcca tcgtcccggc
    11761 ggcgaccttc gccggcgcgc tcgttgtgtg cgcttccata atggccgcgg cgatgcgccc
    11821 ggcgaggaag tcgaagtagt cccggtcgaa tccgggcgcg gcggcggcga accggttgta
    11881 gaagttgctc tcaaagtagc cgccgggacc ggagtgcgtg tgggtccccg cgatcatgag
    11941 ggcgtcggcg cccacgccgg tttccttcgc cacgagctcg gcgacgcgat ggtgcaggat
    12001 gagcgagccg gagaggaggt cgcactgcac cagcgcgatg ggcttcccgg ttttcggttt
    12061 gatgtagatg gcccgcgcgt acaagcgcgt ccggaagccc tcggcggcgc ccgactgctt
    12121 cgaatagccc gccagcggca tgcccggggg cggggtgatg tcgacccgcg ccgccccggc
    12181 gacgagcccg tcggaggggg cgggcgcggc gggcgcgata ttgcgtatgt cgatcttcac
    12241 ctgcggcgcg ctgcaggcat acagcccgag gccgagaccg gcggcgatga tgatgcgtgc
    12301 gatgcgtgtt gtcatggctt cctcgcgtga tggttgatag cgcggcgcga tcgtcccgcg
    12361 tgcgttgcgg ctgcgtaaga cccttgcggg ttgtgcgctc gctttcaagc tttttttcgc
    12421 cgtgtgtcgt cggatagcgc atgtggcgcg gcccggcgac gcgtgcgcga agatgtcgaa
    12481 tgtcggggaa tgctggcggg agactagagc attcccggca ggtgcgattc caggaacttc
    12541 tggtcgtcca ttcccacgag atggcggtac ttctcggatt gaagcgacag cagctcctcg
    12601 ccgcaatcgc agatgaagtt gcgccgcttg gcgtcgtgat ggcagaagta tttctggccg
    12661 tcctggtacg cggggaacac cgcggccatg ctcctgtccg cgagcgcctc tttgcgcgag
    12721 ctcacgatct ccttcgagag caggatggcc cgcataacgc cccgctcgca ggtgatgagc
    12781 cgcgagtact cggtgagtcc ccgctccgat agaaacgagg cgatatcgcg ctcgcaggcc
    12841 ttgtctgcgc attcctgctt ggtgtagcgg ttttcgtaga cgtgcgcgcc gaagcgtttg
    12901 tgcgtgtatt ttccgtaaat gaactttttc tgcttcaagg ccccgaagca caggtaaatc
    12961 tcgttctccg ggtcgaagga gcaagtggcc agcagcagat cgagctcctt gccgcatgcg
    13021 gcaaggagct cgatctgctg ctg
//
LOCUS       DAORHF010000092        13016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3877_length_13016_cov_5.696706, whole genome shotgun sequence.
ACCESSION   DAORHF010000092 DAORHF010000000
VERSION     DAORHF010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_13016_cov_5.696706"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1205
                     /locus_tag="PKJ16_09265"
     CDS             78..1205
                     /locus_tag="PKJ16_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV47219.1"
                     /translation="MKALDRMSDFTLARYRFVFVGLIIILALTVAGALRIKFETNTMN
                     YLPDDNRIKTDINFIEEQFAGTIPFVMLVQAKSPEYDFTHPESLRLVDTIQRDMMKAI
                     THYSTSFSLSDYFKEIHQAFNDGDERFRAIPDNRRDIVDYYELADSEVIDRIVSPDRM
                     ETRLSFQSKWGSNETAKEYHNYIVGYMDKAIGEHYTYKITGLSSMYLNMEFNLKESQI
                     KSILTSFVLIFVMMYFVCGTFWLTVISMVANVFPIVVTLGIMGWFGIPLDVSTIMVAS
                     VTIGIAVDDTIHIVTWLRRHAGSGEKLPDAIRKTYRDVGKPVIITSLVLFFGFMILIL
                     GSIKPTQAFGMLTAFSMLFALIGDLFILPALILVFKPKIAG"
     gene            complement(1278..2042)
                     /locus_tag="PKJ16_09270"
     CDS             complement(1278..2042)
                     /locus_tag="PKJ16_09270"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV47220.1"
                     /translation="MSSDTRELSDFQVRNIRILTHLLEHEAVADDIIEVLRENELDYV
                     SLIDKIGLLEKKVNIDEKTSLLKFKSDYLTTIVKTASRVFYSINKVDYPISFARFDID
                     DFSVFNNRYGHDVGDEVLVRIAAAIKEHSRPTDYVIRFGGEEIDVILPSTDLDGARVY
                     MDKIVESVRRMSVPHNGESLRVTVSGGLTSTLYTFDKSKVINDDDVELVYRELQSRAD
                     DALYEAKFSGKDRYCVFDEARKDDYARFRAQYSRAR"
     gene            2215..2877
                     /locus_tag="PKJ16_09275"
     CDS             2215..2877
                     /locus_tag="PKJ16_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47221.1"
                     /translation="MTSPRKKNDDAYSIDFFDEDGPACEAHAPAFAGLPGGSEVPERL
                     AGLIREAGPVRAAYRIAPLSIDRGVATLGALTLPGATVATLGLEPGAAFPFVITAGAT
                     IDALLDSLTDPLERYRADCLAVARLDGALVRLRERIRAAHATGPLALYLPGNAPEFPI
                     EDIARIIAAIGGAAARLGLRLNDSGTMVPLKSIAGIFLPAAREMDSCEICAMRDACDR
                     RR"
     gene            2880..4667
                     /locus_tag="PKJ16_09280"
     CDS             2880..4667
                     /locus_tag="PKJ16_09280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV47222.1"
                     /translation="MIDAAIADALGSSRMNPATRNIPPTRTGRPLSRARAAAALCAIA
                     LSLAFFACAASGDRTDACIIDLSGEWRIVPGDDAGACPDPGDRRWETIRLPGSIIDHS
                     RKRYGRAEGIVWIRKTVRVDAGCRAGGIGLELGKIGNADRTHANGVFIGSDGRFPPRD
                     FSRWNAARLYFIPESLIGTDGALAITARIAYAVHGEVRGPLRLLDAETWRAVRSRDAL
                     LTDLRYAGMAAVGAAGFIFLLIFIRRPQRQEYLFFALQCVPGFLVIHDGMGTLPIYGS
                     VGAQVKLLGFGWAALVATQLGFLHRLYGLRRRAVDALLWAMLAIVTLTLVFAYDPRAH
                     ERLALGFVIALTSLALYNISIHAQMLARGNAYAKLLIVPGIVLSLSAAHDGIGHLPRF
                     GGPSISIAGYGFQEPVIGYGAAMMLVVAAVILVHRFISAMRAVEDMNVVLERRVRERT
                     AELEKSLHDLSNFIDNTYLDQRIKAPRRYRTAITPAAEEKIKKAILYINENFTEDISR
                     EGLAASLGINPDYLGKLFLVFTGKKIMDYIHELRIKKAVVMLEEGDDTIIDIAFAVGF
                     ESVRTFNRAFHKHLKIRPSQYRRKRTAAE"
     gene            4672..5058
                     /locus_tag="PKJ16_09285"
     CDS             4672..5058
                     /locus_tag="PKJ16_09285"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HNV47223.1"
                     /translation="MKRRIAYLALAALSFILLVLIATRWAGHSFVRGFLGDLLVVVFL
                     FLAVKACVDARSGAVALGVFISACTVETLQFFRLAEALSLAKGSVARIVLGATFDPLD
                     VLAYAIGCVLAYAADRLIRLRLIRKT"
     gene            5375..6205
                     /locus_tag="PKJ16_09290"
     CDS             5375..6205
                     /locus_tag="PKJ16_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47224.1"
                     /translation="MVQVDVVWSYAFGATFAAAAARQIEKEESPFQNKYFTALLLFLS
                     ILFAPSGLYLLWQFPHWETMQVARTWSDIPAWLVCLFGVTNITQGILGFWVSSRLIRG
                     GNTYGANVNWMVAWTIFWSILVMGWDTTGWQRFLYDPTVAGALWTPGAHMGINFFTSN
                     VWLTLVVMGVIIGPALGIPIARWVREGVQSDPAAAARGVPGYANIIAITLVGTFGICL
                     ALAVANGLLVFLIRDLTGSILLAYFIGLPLFWAIAYFVLLRPGRPLHRFAKQFHGGEF
                     "
     gene            6231..7307
                     /locus_tag="PKJ16_09295"
     CDS             6231..7307
                     /locus_tag="PKJ16_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47225.1"
                     /translation="MKKNRNTHRLRDRILGNAWLPLAAFNLFLLAVLSLHAWLNIFPD
                     RGAVAVDPAPPRLAKTLIPSGPITYMHLRNVKFHWDDEIFVRTPDMTVRGEPLGDNRH
                     IDFGYPDSFALNIIGGTVIADLAIVTRLFNTRVFAYPGAPLRNLSLRTLPEEGGAQIE
                     LRGQMKFGAWISFTARAAISVDTESNRIVMTTGSISALGLPFARGLMGSVGLTLEKLV
                     ALDGSRGVAVRGNNIIIDPMALFPPPRLRGLLDTARLEGETLRITFREQERVAFPARP
                     VRAANDIMLFMGTLQIARMRMIDPVVQIIDITPEDFLEFNTARYYTQITASAIRILES
                     QAVLMYMPDIGKLLPAAPRAADAH"
     gene            7447..9930
                     /locus_tag="PKJ16_09300"
     CDS             7447..9930
                     /locus_tag="PKJ16_09300"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV47226.1"
                     /translation="MNRTRTLTHLSFLAALLLFVISPPPCADTTEPPAKKSIRVVASV
                     YEPFVVQKGTAITGFDVDLLDTICRLHGIEYAIRLTSFKEMLDDVAAGRADMAIGCVY
                     VTKERATLFHFTKPYLMGGLVMAVPTSRPLSSVDELDGKRLGVKLNATGERFARRLAE
                     RGIRIRVTPYESTIDSFQALARGELDAVFNDYYNTILLIQRLYAGEISVAKNIWGVRL
                     YDTTPIAFPVRRGNEWLTAILDDEIARLESMGFRERLLAKWFPVQHPVNWPRFALYGA
                     GGVLALVALIALALRRRQQALRTRALEEAAHRFGSLVMTLPLAVFILEHGRILHANTE
                     AGRLMGLHDASESDGKDFDSFFQRSDAGPAGAHSAILERDDPADNADAGGGADTETPI
                     REMIWRRYDGAITPVLCAVHRIEWMGRSARALFAQDLSDYHAVRAEKERLTGLLAQSQ
                     KMEAIGQLAGGVAHDFNNLLTGIMGHADMALLDASDEGQRTPLRHILNAAQRAAELTA
                     KLLTFARRGPMRAAPVHLHAIVEEAIALLPKRFGSAIAITRRLDAERDLVMGDATFLV
                     QVMVNLLINAHDAMDGAGAVAIATDTIVIDERRRALIGCAGTGPHIRLSFADTGCGIA
                     AADLPRIFEPFYTTKASGKGTGLGLAMAYGIVKQHGGELTVESETGRGTTFTLYLPLT
                     EGDVEADRPHGPAALPAGDMSGHIAVIDDEEAVVSYLERLLERGGYAVTSFIDPIAGL
                     AWLRDHYETVDAVLLDVIMPQMDGAELVRVITTERPGLPVLLMSGHVPTEAEVRLGDF
                     REHAASFLAKPLRSAEVLDAIAAALRDKR"
     gene            complement(9952..10506)
                     /locus_tag="PKJ16_09305"
     CDS             complement(9952..10506)
                     /locus_tag="PKJ16_09305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005221767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV47227.1"
                     /translation="MAVVARETGAGPKATHRVFFDIEIGGAPAGRIVFGLFGDTVPKT
                     AENFRALCTGEKGVGKKGKALHYKGCSFHRIIPDFMIQGGDFTEGSGRGGESIYGDRF
                     ADEAFTVRHSVPGLLSMANAGPNTNGSQFFITTVPTPHLDGKHVVFGSVLEGMEVVKK
                     IESEGSPSGQPGKKVIIADCGELK"
     gene            10705..10878
                     /locus_tag="PKJ16_09310"
     CDS             10705..10878
                     /locus_tag="PKJ16_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47228.1"
                     /translation="MKLELNDDERAIIEEMLDKEAQSLPIEIHHTTVFEFKEMLKTKK
                     KAVDDLLGKVRSA"
     gene            complement(10939..11115)
                     /locus_tag="PKJ16_09315"
     CDS             complement(10939..11115)
                     /locus_tag="PKJ16_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47229.1"
                     /translation="MNAIRLNRLLNVGFGCIALFVGVSLALDERSRIIGALLTALAVV
                     YLGFIIRRAAKTRP"
     gene            complement(11219..>13016)
                     /locus_tag="PKJ16_09320"
     CDS             complement(11219..>13016)
                     /locus_tag="PKJ16_09320"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HNV47230.1"
                     /translation="LLGATTIILIFQNTSSAILVGFGFGGSLLALFLRVGGGIFTKAA
                     DVGADLVGKVEAGIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILATAALG
                     AALPWAQGGLKAVLAPMIVAGLGILFSVIGIFAVRTKEQASMKNLLFSLNVGIWGSSF
                     LLIGALIIMAYVNLITWGIFGAVCAGLVAGLIIGQSTEYYTSDDNKPTQGIAQQHTMG
                     PATGILDGISTGMMSAGIPVITIVIGILLAFGFSGGFQNVSLGLYGIGFASVGMLATL
                     GITLATDAYGPIADNAGGNAEMAGLPKEVRTRTDALDMLGNTTAATGKGFAIGSAALT
                     AMALLSAYIEELKLWIGKYAGSGSIKVGSVNFYSEKAFSALAPEVQKATENVSIIKSE
                     LADFVRAYDLTIMNPLLLAGLFLGAMMAFVFCAMAIKAVGRAAGAMVEEVRRQFKEKP
                     GILQGTEKPDYARCVAISTAGAQRKMILPSLLALIVPIVTGLILGVPGVMGLLAGSLT
                     AGFSLACLLNNSGGAWDNAKKFIEKGNFGGKGSDAHKAGVIGDTVGDPFKDTAGPALN
                     ILLKLMTMVSVVFAGVVVKLSPVIGKALGFVK"
BASE COUNT         2268 a         4362 c         4127 g         2259 t
ORIGIN      
        1 tgctctaagg cggcgcgaac gggcgatctg ccgcgccggc tgcggacccg ttcgccgtgc
       61 gggaatcgtc tcgcttcatg aaggcgctcg atcgcatgtc cgatttcacg ctcgcgcgct
      121 atcggttcgt gttcgtcggc ctcatcatca tcctggccct gaccgtcgcc ggggcgctgc
      181 gcatcaagtt cgagaccaac acgatgaact acctccccga cgacaaccgc atcaagaccg
      241 acatcaactt catcgaggag cagttcgccg ggaccatccc cttcgtgatg ctggtgcagg
      301 cgaaatcgcc ggaatacgac ttcacgcacc cggagagcct gcggcttgtc gacaccatcc
      361 agcgcgacat gatgaaggcc atcacgcact actccacctc gttctcgctc tccgactact
      421 tcaaggagat ccaccaggcc ttcaacgacg gcgacgagcg cttccgcgcg atccccgaca
      481 accggcgcga catcgtggac tactacgagc tcgccgacag cgaggtgatc gaccggatcg
      541 tctcgccgga ccgcatggag acgcgcctct cgttccagtc gaaatggggc tccaacgaga
      601 ccgcgaagga gtaccacaat tacatcgtgg gctacatgga caaagcgatc ggcgagcact
      661 acacctacaa gatcaccggg ctctcgtcga tgtacctcaa catggagttc aacctcaagg
      721 agagccagat caagagcatc ctcacctcgt tcgtcctcat cttcgtgatg atgtatttcg
      781 tgtgcgggac cttctggctc acggtcatca gcatggtggc caatgtcttt cccatcgtgg
      841 tcaccctggg tatcatgggc tggttcggca tcccgctgga cgtatccacg atcatggtcg
      901 ccagcgtcac catcggcatc gcggtggacg acaccatcca catcgtcacc tggctgcggc
      961 gtcacgcggg atcgggagag aagcttccgg acgcgatcag gaagacctat cgcgacgtgg
     1021 gcaagcccgt gatcatcacc tcgctcgtgc tctttttcgg gttcatgatc ctgatcctgg
     1081 gctccatcaa gcccacgcag gccttcggca tgctcacggc cttctcgatg ctgtttgcgc
     1141 tcatcggcga tttgttcatt ctgcccgcgc tcatcctcgt cttcaaaccg aagatcgcgg
     1201 gataacgcat cggcgaagcg ggggggcgga gagaggaagc ggaacgttaa ccgttctgcg
     1261 gcgctcgacc gcgcggccta tcgcgcgcgc gagtactgcg cccggaagcg cgcgtagtcg
     1321 tccttccggg cctcgtcgaa cacgcagtag cggtccttcc cggaaaactt cgcctcgtaa
     1381 agcgcgtcgt cggcccgact ctgcagctcg cgatagacca gctcgacgtc gtcgtcgttg
     1441 atgaccttgc tcttgtcgaa ggtgtagagc gtgctggtga ggccgccgct cacggtcacg
     1501 cggagggatt cgccgttatg cggcaccgac atccgccgca cgctctcgac gatcttgtcc
     1561 atgtacacgc gcgcgccgtc caggtcggtc gagggaagga tcacgtcgat ctcctcgccg
     1621 ccgaagcgga tcacgtagtc cgtgggccgc gagtgctcct tgatggccgc ggcgatgcgc
     1681 accagcacct cgtcgcccac gtcgtgcccg tagcggttat tgaagaccga gaaatcgtcg
     1741 atgtcgaagc gcgcgaagga gatgggataa tcgaccttgt tgatgctgta gaagacgcgg
     1801 gaggcggtct tcacgatggt ggtgaggtag tcgctcttga acttgagcag gctggtcttc
     1861 tcgtcgatgt tgaccttttt ttcgagaagg ccgatcttgt cgatgagcga gacgtagtcc
     1921 agctcgtttt cgcggaggac ctcgatgatg tcgtcggcga ccgcctcgtg ctcgagcagg
     1981 tgcgtgagga tgcggatgtt ccgaacctgg aaatcgctca gttctcgcgt atcgcttgac
     2041 atggttcaac tatgcggcac gcttgaaata tttcaagcac aatcccgacg ccgacgcccc
     2101 ccgcggcggg gcgtgatcat cccgacgcgg ccgcgcccct cacgcgtttt ccgcttgccg
     2161 cggcgccgtc ccgctgatac atcggggtcc ggcgcactca caggacagca tgcgatgacg
     2221 tcaccgcgca aaaaaaacga cgatgcgtac tccatcgact tcttcgacga ggacggcccc
     2281 gcctgcgagg cgcacgcgcc ggcattcgcc ggcctccccg gcgggagcga ggtcccggaa
     2341 cggctcgcgg ggctgatccg cgaagcggga cccgtccgcg ccgcctaccg catcgcgccg
     2401 ctttccatcg accgcggcgt ggcgacgctg ggcgccctca cgctccccgg cgcgacagtc
     2461 gcgaccctcg gccttgagcc cggcgcggcc ttcccattcg tcatcaccgc gggcgcgacg
     2521 atcgacgcgc tgctcgactc cctgaccgac ccactcgagc gctatcgcgc cgattgcctc
     2581 gccgtcgcgc ggctcgacgg cgcgctcgtc cggctgcggg agcgcatccg cgccgcgcac
     2641 gcgaccgggc ccctcgccct ctatctgccg ggaaacgcgc ccgagttccc gatcgaggac
     2701 atcgcgcgca tcatcgcggc catcggcggg gccgcagcgc gcctgggcct ccgcctgaac
     2761 gattcgggga ccatggtccc cctcaagagc atcgcgggaa tattccttcc cgccgcgcgc
     2821 gagatggatt cctgcgagat ctgcgcgatg cgcgacgcct gcgaccggcg gcgataaagg
     2881 tgattgacgc ggcgatcgcg gacgcgctag gctcgtcgcg catgaaccct gcgacgcgaa
     2941 acattccccc gacacggacc ggcaggccgc tttcgagagc gcgcgccgca gcggcgctgt
     3001 gcgccatcgc cctctcgctc gccttcttcg catgcgccgc atcgggcgac cgcacggacg
     3061 cctgcatcat cgacctttcc ggcgaatggc gcatcgtccc cggcgacgat gcgggggcgt
     3121 gccccgatcc cggcgaccgg cgctgggaga cgatccgcct gccggggagc atcatcgacc
     3181 attcgcgcaa gcggtacgga cgcgccgagg ggatcgtctg gatacggaag accgtgcgcg
     3241 tggacgccgg ctgccgcgcg ggcgggatcg gcctcgagct ggggaagatc gggaacgccg
     3301 accggaccca tgccaacggc gtcttcatcg gaagcgatgg gcgctttccc ccgcgcgact
     3361 tctcgcgctg gaacgccgcg cggctctact tcattcccga gagcctcatc gggacggacg
     3421 gcgcgctcgc gatcaccgcg cgcatcgcct acgccgtcca cggcgaggtg cgcgggccgc
     3481 ttcgcctcct ggacgccgag acctggcgcg ccgtccgctc gcgcgacgcc ctcctcacgg
     3541 acctgcgcta tgccggcatg gcggcggtgg gcgcggccgg cttcatcttt ctgctcatct
     3601 ttatccggcg accgcagcgc caggagtatc tgtttttcgc tttgcagtgc gtcccgggct
     3661 ttctggtcat tcacgacggc atggggacgc tgccgatcta cggttcggtt ggagcccagg
     3721 tgaagctcct cggcttcggc tgggcggcgc tggtcgcgac ccagctcggc ttcctgcacc
     3781 ggctctacgg cctcaggcga cgcgccgtgg acgcgctcct gtgggcgatg ctcgcgatcg
     3841 tcaccctcac gctcgtgttc gcgtacgatc cgcgcgcgca cgagcggctc gccctgggtt
     3901 tcgtgatcgc gctcacctcg ctcgcgctct acaacatctc catccacgcg cagatgctcg
     3961 cgcggggaaa cgcctatgcg aagctgctca tcgtcccggg gatcgtgctc tcgctcagcg
     4021 ccgcccacga cgggatcggg cacctgccgc gcttcggcgg cccgtcgatc tccatcgcgg
     4081 ggtacggctt ccaggagccg gtgatcggct acggcgcggc gatgatgctc gtcgtcgcgg
     4141 ccgtcatcct cgtccaccgc ttcatcagcg ccatgcgcgc cgtcgaggac atgaatgtcg
     4201 tgctggagcg gagggtgcgg gagcgcaccg ccgagctgga gaagagcctg cacgatcttt
     4261 cgaacttcat cgacaacacc tacctcgacc agcgcatcaa ggcgccccgg cgctaccgga
     4321 ccgccatcac ccccgccgcc gaggagaaaa tcaagaaggc gatcctctac atcaacgaga
     4381 acttcaccga ggacatctcg cgcgaggggc tcgcggcgtc gctgggcatc aatcccgact
     4441 acctgggcaa gctcttcctc gtgttcaccg ggaaaaagat catggactac attcacgagc
     4501 tgcgcatcaa gaaggcggtg gtgatgctcg aggagggcga cgacaccatc atcgacatcg
     4561 ccttcgcggt gggcttcgag agcgtgcgga ccttcaaccg cgccttccac aaacacctca
     4621 agatccgccc cagccagtac cggcgcaagc ggaccgccgc ggagtgagcg catgaagcga
     4681 cggatcgcct atctcgcgct tgccgcgctc tcattcatcc ttctggtcct catcgcgacg
     4741 cgctgggccg ggcactcctt cgtgcgcggc ttcctggggg acctgctcgt ggtcgtcttt
     4801 ctctttctcg cggtaaaggc atgtgtcgat gcgcgaagcg gcgcggtcgc gctcggcgta
     4861 ttcatctccg cctgcaccgt cgagacgctc cagtttttcc ggctggcaga ggcgctctcg
     4921 ctggcgaagg ggagcgtcgc gcgcatcgtg ctgggcgcga ccttcgatcc gctggacgtc
     4981 ctcgcctacg ccatcggctg cgtcctcgcc tacgcggccg accggctgat ccgtctccgg
     5041 ctcattcgaa agacctgatc gcgacaccaa accataccac caaaaagacg aatgagacgt
     5101 caggctatca caacgagcag gcagcccgga ttggctgccg gagccgcaca tacacggatg
     5161 tattgtttgc ggcggggcga gcgtgatagc ctgacgtctc attcgctgac tattggccac
     5221 gaacatattg aaacggattc cagaatgcgc atctccgcat aacggacaat tccagagacc
     5281 gctccgattg tcacgacacc ccggcgctct tcgtgtatat tcgcagcatc gatcgttcat
     5341 cgaatcctac aaaccaatcc ccaggaggac atcaatggta caagtggacg tggtatggag
     5401 ctatgccttc ggcgcgacct tcgcggccgc cgccgcgcgc cagatcgaaa aggaggaatc
     5461 gccgttccag aacaagtatt tcaccgcgct gctgctcttt ctttcgattc tcttcgcgcc
     5521 gtccggactc tatcttctgt ggcaattccc gcactgggag accatgcagg tcgcgcggac
     5581 atggtccgac atccctgcgt ggctcgtgtg cctgttcggc gtcaccaata tcacccaggg
     5641 gatcctcggc ttctgggtgt cgagcaggct catccggggc ggcaacacct acggcgcgaa
     5701 cgtgaactgg atggtcgcgt ggacgatctt ctggtccatc ctggtgatgg gctgggacac
     5761 gacgggctgg cagcgattcc tgtacgaccc cacggtggcg ggcgcgctct ggacgccggg
     5821 tgcgcacatg gggatcaact tcttcaccag caatgtgtgg cttacgctgg tggtcatggg
     5881 cgtcatcatc ggtcccgcgc tggggattcc catcgcccgc tgggtgcggg agggcgtgca
     5941 gtccgatccc gccgcggccg cgcgcggcgt gccgggatac gcgaacatca tcgcgatcac
     6001 gctcgtcggc accttcggga tctgcctggc cctggccgtc gcaaacgggc tcctggtgtt
     6061 cctgattcgc gacctgacgg gcagtattct gcttgcctat tttatcggac tgcccctctt
     6121 ctgggcgatc gcgtacttcg tgctgctgag gccgggacgg cccctgcacc ggttcgcgaa
     6181 gcagttccac ggcggggaat tctaacggcg ccgcgacggc gacagggacc atgaaaaaga
     6241 acaggaatac gcaccgcctt cgtgaccgga tactcggcaa tgcatggctg ccgctcgcgg
     6301 cgttcaacct gttcctcctc gcggtcctgt cgctgcacgc atggctcaac atcttcccgg
     6361 accgcggcgc ggtcgcggtc gatccggccc cgccgcgcct ggcgaaaacg ctcatcccgt
     6421 ccggcccgat cacctacatg cacctgcgca acgtgaagtt ccactgggac gacgagatat
     6481 tcgtccgcac gcccgacatg accgtgcggg gcgagcccct gggcgacaac cggcacatcg
     6541 acttcggcta cccggactcg ttcgcgctca acatcatcgg cgggaccgtg atcgccgatc
     6601 tcgcgatcgt gacgcggctg ttcaacacgc gcgtcttcgc ctatcccggc gcgccgctgc
     6661 gcaacctctc gctgcgcacg ctgccggagg agggcggcgc gcagatcgag ctccgcggcc
     6721 agatgaaatt cggcgcctgg atctcgttca cggcgcgcgc cgccatatcg gtcgacaccg
     6781 aatcgaaccg catcgtgatg accacgggat cgatctcggc gctggggctg cccttcgccc
     6841 ggggactgat gggcagcgtg gggcttacgc tcgaaaagct cgtcgcgctc gacggttcgc
     6901 ggggcgtcgc ggtccggggc aacaacatca tcatcgatcc gatggcgctc ttcccgccgc
     6961 cgcgcctgcg cggtctgctg gacacggccc gcctcgaggg cgagacgctg cgcatcacct
     7021 ttcgcgagca ggagcgcgtc gcgtttcccg cccgtccggt gcgcgcggca aacgatatca
     7081 tgctcttcat ggggacgctg cagatcgcgc gcatgcgcat gatcgacccg gtggtgcaga
     7141 tcatcgacat cacccccgag gacttcctcg agttcaacac cgcccgctat tatacgcaga
     7201 tcaccgccag cgcgatccgc atcctggaga gccaggccgt gctcatgtac atgcccgaca
     7261 tcgggaagct gcttcccgcc gcgccccgcg cagccgacgc ccactaaccg cgccgcaccc
     7321 ggcccgattt catcgcgtgg cgcagcgcca gaaagaatct tgacgtgatt tgaaacctct
     7381 tatacactcg cagtaccgag ggcggaacgc ccgtggatac acagaagtcg cctacacgat
     7441 agcggaatga accgaacccg tacattgacg cacctgtcgt ttctcgcggc gctgctgttg
     7501 ttcgttattt caccgccccc ctgcgccgac acgacggaac cgcccgcgaa gaagagcatc
     7561 cgcgtggtcg cctcggtata cgagccgttc gtggtgcaga aggggaccgc gatcaccggc
     7621 ttcgacgtgg acctcctgga cacgatctgc cgcctccacg gcatcgagta cgccatccga
     7681 ctcacctcct tcaaggagat gctcgacgac gtggcggcgg gacgcgcgga catggccatc
     7741 ggctgcgtct atgtcacgaa ggagcgcgcg acgctctttc atttcacgaa gccctatctc
     7801 atggggggat tggtgatggc ggtgcccacc tcacgtccgc tctcgtccgt cgacgagctc
     7861 gacggcaagc gcctgggggt caagctcaat gcgaccggcg aacgctttgc gcgtcggctg
     7921 gctgaaaggg gaattcgcat ccgggtgacg ccctacgagt cgaccatcga cagcttccag
     7981 gcgctcgcgc gcggcgagct ggacgcggtc ttcaacgact actacaacac catccttctc
     8041 atccagcggc tctacgccgg cgaaatctcc gtggccaaaa acatctgggg cgtgcgcctg
     8101 tacgacacca cccccatcgc ctttccggtg cgccgcggca atgaatggct cacggcgatc
     8161 ctcgacgacg agatcgcacg gctcgagagt atgggctttc gcgaacgcct gctcgcgaaa
     8221 tggtttcccg tccagcatcc ggtcaactgg ccgcgcttcg ccctctacgg cgccggcggg
     8281 gtccttgcgc tcgtcgcgct catcgcgctg gcgctccggc gacgccagca ggcccttcgc
     8341 acgcgcgccc tcgaggaggc ggcgcaccgc ttcggctccc tggtcatgac cctgcccctg
     8401 gcggtgttca tactcgaaca cggaaggata ctgcacgcga atacggaggc ggggcggctg
     8461 atggggcttc acgacgcgag cgaatcggac ggcaaggatt tcgactcctt cttccagcgg
     8521 tccgatgcgg gtccggccgg cgcacactcc gccattctcg agcgcgatga tcccgccgac
     8581 aacgcggacg cgggcggcgg cgccgatacc gaaacgccga tccgcgagat gatctggcga
     8641 cggtacgacg gcgccatcac cccggtcctc tgcgccgtgc atcgcatcga gtggatgggc
     8701 agaagtgccc gggcgctctt cgcgcaggac ctgagcgact accacgccgt ccgtgcggag
     8761 aaggagcgcc tcacgggcct gctcgcgcaa tcgcagaaga tggaggccat cgggcagctg
     8821 gcgggcggcg tggcgcacga cttcaacaat ctgctcaccg gcatcatggg ccatgccgac
     8881 atggcgctcc tggacgcatc ggacgagggg cagcgaacgc ccctccggca catcctgaac
     8941 gccgcgcaac gcgccgcgga gctcaccgcg aagctgctca ccttcgcgcg ccgcgggccg
     9001 atgcgcgccg cgccggtcca tctgcacgcc atcgtcgagg aggccatcgc cctccttccg
     9061 aagcgcttcg gaagcgccat cgccatcacc cgtcggctcg acgccgaacg cgacctggtc
     9121 atgggcgacg cgaccttcct ggtgcaggtg atggtaaacc tgctcatcaa cgcgcacgac
     9181 gccatggacg gcgcgggcgc cgtcgccatt gcgaccgaca cgatcgtcat cgacgagcgc
     9241 cgccgcgcgc tcatcggctg cgccgggacc ggtccgcaca tccggctctc cttcgcggac
     9301 accgggtgcg ggatcgccgc ggcggacctc ccgcgcatct tcgagccgtt ttacaccacc
     9361 aaggcgagcg ggaagggaac ggggctgggg cttgcgatgg cctatgggat cgtgaaacag
     9421 cacggcggcg agctgacagt ggaaagcgaa acgggacggg gcaccacgtt caccctgtat
     9481 ctgccgctca ccgaaggcga cgtcgaggcc gatcggccgc acggcccggc cgccctgccc
     9541 gccggcgaca tgagcggcca catcgcggtc atcgacgacg aggaggcggt ggtgagctac
     9601 ctggagcgcc tgctcgagcg gggcggatac gccgtcacgt cattcatcga cccgatcgcg
     9661 gggttggcct ggctgcggga ccactacgag acggtggacg ccgtgcttct ggacgttatc
     9721 atgccgcaga tggacggcgc ggagcttgtg cgcgtcatca caaccgagcg cccgggcctc
     9781 ccggtgctgc tcatgagcgg ccacgtcccc acggaagccg aggtgcggct cggcgatttt
     9841 cgcgaacacg ccgcgtcgtt cctggccaag ccgctgcgca gcgccgaggt gctcgacgcg
     9901 atcgcagcgg cgttgcgcga caaacgatag aaaccccgac ccggcgcgcg gctacttgag
     9961 ttcgccgcag tccgcgataa tgaccttctt cccgggctgc ccgctcgggc ttccctccga
    10021 ctcgatcttt ttcacgacct ccatgccctc gagcaccgag ccgaacacga cgtgcttgcc
    10081 gtcgagatgg ggcgtgggga ccgtggtgat gaagaactgc gaaccgttgg tgtttgggcc
    10141 ggcgttcgcc atgctcagca ggcccgggac cgagtgccgg acggtgaagg cctcgtcggc
    10201 gaatcgatcg ccgtagatgc tctcgccgcc gcgtccgctt ccctcggtga aatcgccgcc
    10261 ctggatcatg aaatcgggga tgatacggtg aaaggagcac cccttgtagt gcagcgcttt
    10321 cccctttttt cccacgccct tctccccggt gcacagcgcg cggaagttct cggcggtttt
    10381 cgggaccgtg tccccgaaca gaccgaatac gatccgcccg gccggcgccc cgccgatctc
    10441 gatatcgaaa aatacccggt gcgtcgcctt cggaccggcg ccggtctcgc gcgcaacgac
    10501 cgccatcgcc accagcgcaa ccgacagcag gccgaacgca atatggaaca gaatactcat
    10561 tatactcacc tctttgccgt tgtagagcgc tcaggaggca tgcgcgggcc cgctctgtca
    10621 aacggaaaaa ccggggtttt ttttgactcg ggtccggccg ttcactatgt tccccatgga
    10681 gaacattcgc gaaggagggg gagcgtgaaa ctggaattga acgacgacga acgcgcaatc
    10741 atcgaggaga tgctcgacaa ggaggcgcaa tcgcttccga tcgagatcca ccacaccacg
    10801 gtgttcgaat tcaaggaaat gctcaagacg aagaagaagg cggtggacga tcttctgggc
    10861 aaggtgcgct cggcataggc gcgcctgcgc cgcatcgcgc tgaggcggct gggcgcacat
    10921 gggacgcggg gcgtgggcct acgggcgggt tttggccgcg cgccggatga tgaacccgag
    10981 atagacgacc gcaagggcgg tgaggagcgc gccgatgatg cgggagcgct cgtcgagcgc
    11041 gaggcttacg cccacgaaca gcgcgatgca cccaaacccg acattgagca ggcgattaag
    11101 ccggatagca ttcattccga tacctccgcg gcgaccatgc gggcgccgtc aataaatcga
    11161 aggccgcccg aacgggcggc cttcgcagta cttcgcatta caaaacttcc ggcaacaatt
    11221 acttcacgaa accaagcgcc ttgccgatga ccggcgaaag cttgacgacg acgccggcga
    11281 aaacgaccga caccatggtc atgagcttga gaagaatgtt gagggccgga ccggccgtgt
    11341 ccttgaacgg gtcgccgacg gtgtcgccga tgacgccggc cttgtgggcg tcggagccct
    11401 tgccgccgaa gttgcccttc tcgatgaact tcttggcatt gtcccacgcg ccgccggagt
    11461 tgttcagcag gcaggcgagc gagaagccgg cggtcagcga gccggcgagg aggcccatca
    11521 cgcccggaac gcccaggatg aggccggtga cgatcggaac gatgagcgcc aggagcgacg
    11581 gcaggatcat cttgcgctgg gcgcccgcgg tggaaatcgc gacgcagcga gcataatccg
    11641 gcttttcggt gccctgcagg atgccgggct tctccttgaa ctggcggcgc acctcttcga
    11701 ccatggcgcc ggcggcgcgg cccacggcct tgatcgccat cgcgcagaag acgaacgcca
    11761 tcatcgcgcc gaggaagagg ccggcgagaa gaagcgggtt catgatcgtc agatcgtagg
    11821 cgcgaacgaa gtcggccagt tcggacttga tgatggacac gttttcggtc gccttctgca
    11881 cctcgggtgc gagcgcgcta aacgccttct ccgagtagaa attgacgctg ccgaccttga
    11941 tggagccgga gccggcgtac ttgccgatcc acagcttgag ctcttcgatg taggcggaca
    12001 gcagcgccat cgcggtgagg gcggcggaac cgatcgcgaa acccttcccg gtcgcggcgg
    12061 tggtgttgcc cagcatgtcg agcgcgtcgg tgcgggtgcg cacttccttg ggaagaccgg
    12121 ccatttcggc gttgccgccc gcgttgtccg cgatggggcc gtacgcgtcg gtggccaggg
    12181 tgatgcccag cgtggccagc atgcccacgg aggcgaagcc gatgccatag aggccgagcg
    12241 atacgttctg gaagccgccg gagaacccga aggccaacag gatgccgatc acgatggtga
    12301 tgacggggat gcccgccgac atcatgccgg tggagatacc gtcaaggata ccggttgcgg
    12361 gacccatggt gtgctgctgt gcgatgccct gggtgggctt gttgtcgtcc gaggtgtagt
    12421 actcggtgga ctggccgatg atgagacccg caaccagacc cgcgcatacc gcgccgaaga
    12481 tgccccaggt gatgaggttc acatacgcca tgatgataag cgcgccgatg agcaggaagg
    12541 agcttcccca gatccccacg ttgagggaga agaggaggtt cttcatggac gcctgctcct
    12601 tggtgcggac ggcgaagatc ccgatgaccg agaagaggat gcccagaccg gccacgatca
    12661 tgggggccag gaccgccttc aggccgccct gcgcccacgg gagcgcggcg ccgagcgcgg
    12721 cggtggcgag gatggagccg caatacgatt cgtagaggtc ggcgcccatg ccggccacgt
    12781 cgcccacgtt gtcgccgacg ttgtccgcga tcgtcgcggg gttgcgggga tcgtcctcgg
    12841 ggatgccggc ctcgaccttg ccgaccaggt cggcgccgac gtcggcggcc ttggtgaaga
    12901 tgccgccgcc gacgcgcagg aacagcgcga gcagcgagcc gccgaagccg aagccgacca
    12961 agatggccga gctggtgttc tggaagatca agatgatggt ggtggcaccc agcagg
//
LOCUS       DAORHF010000093        12999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3882_length_12999_cov_6.270164, whole genome shotgun sequence.
ACCESSION   DAORHF010000093 DAORHF010000000
VERSION     DAORHF010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3882_length_12999_cov_6.270164"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PKJ16_09325"
     CDS             complement(<1..1080)
                     /locus_tag="PKJ16_09325"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV47231.1"
                     /translation="MRDKTINLGNLLLSLSEAIDLANPLIALHQQRTSYIALRIAEQD
                     GIAGQPLRDLFIAALFHDTGALTVEEKTALHEFEEVDVGNHCVKGEILFKRIPMFDRA
                     APIIRFHHIRWDAATPSACTDAVREAAHIVCLADYIERLVKRTGYILGQVDPLVERIR
                     SLSGSELDPIHVDAFQRLARSESFWLDLVSPKLYSLLLHKGPLAGETIHAAALEEIST
                     LFRDFIDFKSSFTAAHSSGVSKCAETIGDLLGVTDDERFDLRIAGNLHDLGKLIIPNA
                     ILDKNGPLTDEEYRLMRCHPYHTFHVINTVSGLSRIAAMAGHHHETLDATGYPFQRPA
                     QELMTGERIIMVADIFTALIEDRPYR"
     gene            complement(1514..2548)
                     /locus_tag="PKJ16_09330"
     CDS             complement(1514..2548)
                     /locus_tag="PKJ16_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47232.1"
                     /translation="MKAMRLFPVITCFLFLPGAAERTAFSTNSAVRLLKPIPTESRTQ
                     ADIIARWKQAKPTFTGNPYLELPSLAPPHSPGKLDPDFLNDGLKMLNFIRYLAGIPDA
                     VTLDASLNETCQSACVLMAATRKIAHAIGKPANMSASFHTKAAVGLRRSNLRYAGKPI
                     TLDRAVRDWTDDSDRSNIDRLGHRSGILTYLMRKTGFGMAGQYSAVDVRDYEVSGAVT
                     WPEHHEFMCWPAAGYFPVQMMHRGTAWKITLRFNFDPVSKPFSKYSLTLKRMNDGRIW
                     KFSEGDTKYTRDGNYISSYVGPVFIFRPDASFTCNPGDIYEVSLKGLELKPGEHAEIR
                     YQVKLFSLPE"
     gene            complement(2795..2868)
                     /locus_tag="PKJ16_09335"
     tRNA            complement(2795..2868)
                     /locus_tag="PKJ16_09335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2832..2834),aa:Arg,seq:cct)
     gene            complement(2885..3526)
                     /locus_tag="PKJ16_09340"
     CDS             complement(2885..3526)
                     /locus_tag="PKJ16_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV47233.1"
                     /translation="MMLDGQLFDKFGVEYAPNEIIFCEFEPGNEFYFIHSGRVKIVKI
                     INNTEKTLDILEPGDVFGEMAILEEEEPRSASAIAAEQVKLLKFHRANFDALLQGNPQ
                     LAYKLLVIFSKRIYDAKRRLMILLLDEPQLKVMDVFNMLSENNPQIDIQAPVTLYATT
                     QEVANWAGLPLNDVQKVLTQFSKLGKIELFNDRIVVNNIRDFQRVVSSKRKKL"
     gene            complement(3546..4802)
                     /locus_tag="PKJ16_09345"
     CDS             complement(3546..4802)
                     /locus_tag="PKJ16_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV47234.1"
                     /translation="MSNGSSPVSGIITRRYKTGSIIYFENDKSEYIYILKEGKVVLTS
                     TKVEKGDIVETKDFVKPGEFFGVKSALGKYPRDETAQTASEVITLVLTPAEFERLILK
                     NVAVVKKMLRVFSNQLRRITKMVREVLGGGTTLNPEQELFKIGEYYFKNGKHQQAIYA
                     YKKYMEYYPGTKFAPVAMKRIEAINTGSADYDMMQDSVSFDSGSASGSSGGSDADMTD
                     FSIDEPDEPTSAPSDDFDFDEPAPAAPAGGGSPKSALHNEMDDFLSDDKASGLDDFSF
                     DDDLGGASAPEPPKDITQQYYEAMNLYSQDRAADALALYKKILESKTLKTDADKKFYE
                     KAHFELGRCQLKLNKSPDALAAFSALLKKFPTTDLAKNAYLHIGLAYEKQRDVENAKK
                     FYSRAAATEPRDDINIQAAKRLKALQ"
     gene            complement(4824..6053)
                     /locus_tag="PKJ16_09350"
     CDS             complement(4824..6053)
                     /locus_tag="PKJ16_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV47235.1"
                     /translation="MGKWGLTGATIVTPDETIKNAGIAIQGDKIERVSGGPVRGVDAF
                     DAGDTVLAPGLINAHDHLLGTYYPKVGNGPYENWLPWDNDLKSALVYEERQQIENRDL
                     YLLGAYRNLVSGVTIVSDHIPHFVSEPFLDLLPVKAIREYALAHSVASFALAWGDEIA
                     VEYQKAIDKDIPFITHIAEGFDDETKQDLKTLERRGGLGDHSVLVHGIAFTESDIETI
                     KKKGASVVWCGDSNTFMFNATANVKKMLEAGVNLCIGTDSPMSGGLNLLFEMKFDKDY
                     YTKTYGAELSDERIVKMVTSNPAHAFKLKKNGHIKPGYLADFALFAKKSANPYSAIVQ
                     AEIRDVELVVVNGRPAFGSARFRGLFDSQKVACQEVTIDGARKLVVGDLTGLLRRINR
                     AVGFKKKLPFLPVELDG"
     gene            complement(6198..8303)
                     /locus_tag="PKJ16_09355"
     CDS             complement(6198..8303)
                     /locus_tag="PKJ16_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47236.1"
                     /translation="MKSSIAVIAMLALLVPTAAARATDDDRRAWWDDRVRELRAERTA
                     RTARTPLELRLRDALDRDITRAESMRAAFDRSAGGATSARADAPIAPARVRALAAEIV
                     PPIIALHTLDRILARHDPIEPDPGLRRIVGERCAERLRAADPGIAPDAVGRLVSDHIR
                     AEEWRLIAWELFMDRAISEHDARTASLVGRCADETIAALERKTIPAVDRSVRAHIAAR
                     AMTHGAQMDIEGSARIGASTVAENVAARLVRMQRLALGPSASTSAAHRAAVEGELHKR
                     SIAIRAAIAFLESLVGARQSLPEAALAPSLTLVSAQNKRIAALAKRLDAAGAIDAADR
                     RFLDRAALTRHRETRSQSREDAKRLVAHAGLAAKHLTQKQAQVTADGTRARQRLRERL
                     AHGEIDSLMESAQDLLAIYRAITHADDAFARYQDAFAGMQNAASDGAQHPGLDCALQA
                     GSVIPAVPHFDAERITAEHRIKSDLRKHIMLRLAQASRLAKTYAQRGVSMKNMITPER
                     TAEITRALSRESAARIASWRMDESNVAAIDRKAVEHLRAIRNRQAWRDGAGKLAGART
                     VSVPEAGITLTLPASVVERAPIGAEIEKGVIRAFATPDGKGVFYVSRPAPLDAAPEDA
                     TDLWLSTMGNTAVSDRWGTQESRAYYWTLSRDRRAQVMETMTVNGARGPVIIAGVTTK
                     SRYNFFKQHIDALFQSVKF"
     gene            complement(8417..9760)
                     /locus_tag="PKJ16_09360"
     CDS             complement(8417..9760)
                     /locus_tag="PKJ16_09360"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV47237.1"
                     /translation="MKTDTALGIIKFRRRRRIMMRNTLFVFLCYALTQALSLVAWATG
                     ISSISPREIAAIAGASLGISIVFLAVIASRKGEITTAFANAVFFGQFAFWLVLYLLWI
                     LALNEIRIAGLFFAILALSFTLSNTNLLQSLSIALATAMVQIAGTYFAIVVLRQKGAL
                     FTEIFFTLCFLPAALFITYLADQFQRQRDEIKAAKRTAERARDALWGEMRLAEKIQTV
                     LLPQHPSIPGYDVSAFMKPAEEVGGDYYDIINTPAASWVVIGDVSGHGVSAGIIMMMV
                     ESIIQAVVRERPTVPPGELLRIANRAISHNIRRMDEENYMTITALSIDADGAALHAGL
                     HLDILVYRARDGAVESIPTEGMWLGIRPEIGEFTRDGSFQIDPGDVMLLYTDGVIEAK
                     DVLGAQFGGAALARILAAHGRDDTRTILEKIIEQLSLYRPDDDATLVILKRLARD"
     gene            9893..11170
                     /locus_tag="PKJ16_09365"
     CDS             9893..11170
                     /locus_tag="PKJ16_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47238.1"
                     /translation="MTQNAETQLIPFDRMKEQVERAIAAFLEQTRSPEAVPAPRIAFE
                     KGSTIIAFPLPFLNEALLASFIGLIKTHLANVRIHAFGDGYYAFQSLGGNLFRTENIL
                     DNVKIEFSGLTTSFKAEISKKGGLTQEEVNLAVELYKEAYSAVKDDPIARLRAMGASV
                     YTDNGELTWAYIAGYDEVKKKIRESIILPLTNPEMYNSIARLTRKTFETNRPKAILFE
                     GSPGVGKTTVARILAGEVSVPLVYVPIESIMSKWYGQSSQNLAGIFDAAEDLGGSILF
                     LDEIDALAGSRDQNMFEATRRVLSVLLRKLDGIDSVVSTVTIGATNRKGDLDHALISR
                     FDQSIYFPLPNERERAAILANYAAHLSDEERAGLAGIIDGLSGRTIKDICEMAERRWA
                     RRLIVKGMEPSPPPPEHYRQSARTWIEDNRVRD"
     gene            complement(11212..>12999)
                     /locus_tag="PKJ16_09370"
     CDS             complement(11212..>12999)
                     /locus_tag="PKJ16_09370"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HNV47239.1"
                     /translation="ATPAELWPIVSDTSRLNELMGYPSMSFTERNGRRFGSYRLAGMR
                     QEWEEPPWEWEAERSITMTRIFSKGIARYLRGMIDIEPALPGTARVQVYFGWIPRHLP
                     ARLLLIGARTSFGNRFANAFAALVPERTLAGAIILQPVDYLRQTSTGTAANGERLAIA
                     KHALLADGGDPDTTVKLAAHIAAAPDVELHRMRPRALARTFATGEEATLGTMLRGTRR
                     GMLSMNWDVVCPHCRGVRARINHLWEVPAKGRCDACQVDFDTSSLNAVEITFQPRPEI
                     RRVEEVLYCSAEPARKPHIRIQRAVDAESVLALPLALAPGRYRLRTVGGTIYHLLDVR
                     EDAPRREILWDADATAAAFETGSGAVLVMRNPGTAPRTFVVEEHELDRDALRPRDLFN
                     LQDFRDLFADESLPADLSIDVGMQNIMFVDIVGSTVMYGREGDSRAFTIVRAFFRASH
                     DLAAAHRGAIIKTMGDAVMASFDDPMDALRCARAFVTTFDGRDPEAPLRTRVTINRGP
                     CLAVNLNSAIDYFGQPVNVAAKLQPFAGAGEIALAEAFMEDEAVRAYFAAKGHARPPL
                     RAAEIAGAGTVRYLVMRPINRTRGDDSEA"
BASE COUNT         2189 a         4000 c         4343 g         2467 t
ORIGIN      
        1 gcggtagggg cgatcctcga tgagcgcggt aaaaatgtcg gccaccatga tgatccgttc
       61 gccggtcatc agctcctgcg cggggcgctg gaagggatac ccggtcgcat cgagcgtttc
      121 atgatgatgg cccgccatgg cggcgatccg cgaaagcccg ctgacggtgt tgatgacgtg
      181 gaacgtgtgg tacgggtggc atctcatcag ccggtattcc tcgtcggtca gcgggccgtt
      241 cttgtcgaga atggcgttgg ggatgatgag cttccccagg tcgtgcaggt ttccggcgat
      301 gcgaagatcg aaacgttcgt cgtccgtcac gccgagaagg tcgccgatgg tttccgcgca
      361 cttcgagacg ccgctggagt gcgccgcagt gaacgaagac ttgaagtcga tgaaatccct
      421 gaacagcgtg gaaatttcct cgagggcggc cgcgtggatc gtctctcccg ccaggggccc
      481 tttgtggagc agcaaggagt agagcttggg cgatacgagg tcgagccaga agctctcgct
      541 tcgcgcaagc cgctggaacg cgtccacatg gatcgggtcc aactccgatc ccgagaggga
      601 gcggattctc tcgacgagcg gatcgacttg cccgagtatg tagccggtgc gtttgaccag
      661 tcgctcgata taatcggcca ggcagacgat atgagccgct tcccgaacgg cgtcggtgca
      721 ggcggatggg gtggcggcgt cccaccggat gtgatggaag cggatgatgg gggccgcccg
      781 gtcgaacatc ggaatccgct tgaagaggat ctcgcccttc acgcagtgat tgccgacatc
      841 cacctcctcg aattcgtgca gggccgtttt ctcctcgacg gtaagggcgc cggtatcgtg
      901 gaacagggcg gcaatgaaga ggtcgcgtag cggttgtccc gcaataccgt cctgctccgc
      961 gatccgaagc gcgatatacg atgtacgctg ctgatgaagc gcgatcagcg gattggcgag
     1021 gtcgatcgct tccgagagcg agaggagcag gtttccgagg ttgatcgtct tgtcgcgcat
     1081 cgatgaaata tcccgaagtt gaaacgagcc ggttgtcgaa catgcattgt ccatcgtcgg
     1141 tgtcgcacgc gatgtgaatc ctgcgatggc ggtgcacttc gaagatagac atcatatcac
     1201 gcaatgggtt tccgtaaaac atttttccgc gcaatcgaat cttttatgga tggttcgcca
     1261 agatttgcgt cattgtcgcg aacgcacgat tggcactcga tgcgggtgga tgccgcacgt
     1321 cgagccatgc aatatgaaac ggttcgaatt agccgggagc gtgtgattta cgtgtgaaga
     1381 tttccgtagg gctttattta ttcaaagggc gctggacgtt ttctccgaag atggttcgga
     1441 taaacgatca aatcgccgcg ctttacttac gatgatatcg aggtttgaaa aatttttcac
     1501 tggaaaagac ggttcactcg ggcagcgaaa agagcttcac ctggtacctg atctccgcat
     1561 gctcgccggg tttgagctcc aagcccttca ggctgacctc gtatatgtcg ccgggattgc
     1621 aggtgaacga cgcatcgggg cggaaaataa acaccggtcc cacatacgat gaaatatagt
     1681 tcccgtcccg ggtgtatttc gtatcgccct cgctgaattt ccatatcctg ccgtcattca
     1741 tccgcttcag ggtaagggag tatttcgaga atggcttgct gacgggatcg aagttgaacc
     1801 tgagggtgat cttccatgcg gtcccccggt gcatcatctg cacggggaaa tacccggccg
     1861 ccggccagca catgaattca tggtgctcgg gccaggtcac cgctccgctc acctcgtaat
     1921 ctcgtacgtc aaccgccgag tactgcccgg ccatcccgaa cccggttttc ctcatcagat
     1981 aggtgagaat tccgctccgg tgcccgagcc tgtcgatatt gctccggtcc gaatcgtcgg
     2041 tccagtcccg gacggcgcgg tcgagcgtga tgggtttgcc cgcataccga agattggaac
     2101 ggcgcaggcc caccgcagcc ttcgtgtgga acgatgcgct catgttcgcc ggtttgccga
     2161 tcgcatgggc gattttccgc gtcgcggcca tcagcacgca cgcgctctgg catgtttcat
     2221 tcaatgaggc gtcgagcgtg acggcatccg gtatgccggc gaggtatcgt atgaagttca
     2281 gcattttaag gccgtcgttc aggaagtcgg gatcgagctt tccgggcgaa tggggcgggg
     2341 cgagcgacgg cagttccaga tacggattgc cggtaaaggt cggtttggcc tgcttccacc
     2401 tcgcgatgat gtcggcctgc gttctcgatt ccgtcggtat cggcttgagc aacctgaccg
     2461 cgctgttcgt ggaaaacgcc gttcgttccg ccgctcccgg caggaaaagg aaacaggtga
     2521 tcacggggaa aagccgcatt gccttcatgt ggtcctccat tccgatatat cgtaaatatg
     2581 ccgtcgccat tgcgcgttgt aggtcccaat gggaactcat cgcgcagcag gtcttgtgcc
     2641 gtcaaccggg catttgtccc gtggggaaac gacagacgac acggaaacga gatgaaggaa
     2701 cacgggagga agccttcagg gaatcggaac ggcgctctat ggtgcgcccg ttcgcgggcc
     2761 gttcgtttcc gtgcatttgg cgttttgggg actgtgctcc cgggaggact cgaacctccg
     2821 gcacatagtt taggaaacta ctgctctatc cacctgagct acgggagcgg tgcggtcgcg
     2881 acggtcacag cttctttcgc ttggagctca cgacgcgctg aaagtcgcgg atgttgttca
     2941 ccacgatgcg gtcgttgaac agctcgatct tcccgagctt gctgaattgt gtcaatactt
     3001 tttggacgtc gttgagcggc agccccgccc agttggcaac ctcctgcgtg gtcgcgtaga
     3061 gcgtcactgg cgcctggatg tcgatctgcg ggttgttctc ggagagcatg ttgaacacgt
     3121 ccatcacctt gagctgcggt tcatcgagca gaaggatcat cagccgccgc ttggcgtcgt
     3181 agatacgctt ggagaagatc accaggagct tgtaggccag ttgcgggttt ccctgcagga
     3241 gcgcgtcgaa gttggcgcga tggaacttga ggagcttcac ctgctccgcc gcgatcgccg
     3301 aggccgagcg cggctcctcc tcctcgagga tcgccatctc cccgaacacg tcccccggct
     3361 cgaggatgtc cagggtcttt tccgtgttgt tgatgatttt cacgatcttc acgcgaccgc
     3421 tgtggatgaa gtagaactcg ttgccgggct cgaactcgca gaagatgatc tcgttgggcg
     3481 cgtactcaac cccgaatttg tcgaatagct ggccgtctag catcatggcg tgtctccggt
     3541 gtgtctcact gcagggcctt caggcgcttg gccgcctgga tgttgatatc gtcccgcggc
     3601 tcggtcgcgg ccgcgcgcga gtagaatttc ttcgcgttct cgacgtcgcg ctgcttctcg
     3661 taggccaggc cgatgtgcag ataggcgttc ttggccaggt cggtggtggg gaacttcttg
     3721 agcagcgccg agaaagcggc aagcgcgtcc ggcgacttgt tgagcttgag ctggcagcgg
     3781 cccaactcga agtgcgcctt ctcgtagaac ttcttgtccg cgtcggtctt cagggtcttg
     3841 gactccagga tcttcttgta cagcgccagc gcgtcggcgg cgcggtcctg cgagtagagg
     3901 ttcatcgcct cgtagtattg ttgggtgatg tccttgggcg gctccggcgc gctcgcgccg
     3961 ccgagatcat cgtcgaagga gaagtcgtcc aggcccgacg ctttgtcgtc cgagaggaaa
     4021 tcatccatct cattgtggag cgcggacttc ggcgaaccgc cgccggcagg ggctgcgggc
     4081 gcgggctcgt cgaagtcgaa atcgtcgctc ggggcggacg tcggctcgtc gggctcgtcg
     4141 atgctaaagt cggtcatatc cgcgtcgctc ccgccggagg atccggaggc ggacccggaa
     4201 tcgaacgaga cgctgtcctg catcatgtcg taatccgcgg aaccggtgtt gatcgcctcg
     4261 atgcgcttca tggcgacagg cgcgaacttg gtgccgggat agtattccat gtatttcttg
     4321 taggcgtaga tcgcctgctg gtgcttgccg ttcttgaagt agtactcgcc gatcttgaac
     4381 agctcctgct cgggattgag cgtagtgccg ccgccgagca cttcgcgcac catcttggtg
     4441 atgcggcgta gctgattgct gaagacgcgc agcatctttt tgaccaccgc gacgttcttg
     4501 aggatgaggc gctcgaactc ggcgggggtg agcaccagcg tgatcacctc gctcgcggtc
     4561 tgcgccgtct cgtcgcgcgg gtacttgccc agcgccgact tcacgccgaa gaactcgccc
     4621 ggcttcacga aatccttggt ctcgacgatg tcgcccttct ccaccttcgt ggaggtgagg
     4681 accaccttgc cctctttgag gatgtagatg tactcgctct tgtcgttctc gaaatagatg
     4741 atggagccgg ttttatagcg gcgggtgatg atgccggaaa ctggagacga cccattggac
     4801 atgacgcttc cccctcggaa tacctacccg tcgagctcga ccggcaaaaa cggaagtttc
     4861 tttttaaatc ccaccgcgcg gttgatcctg cgcaggagcc ccgtcaggtc gcccacgacg
     4921 agcttgcgcg cgccgtcgat cgtgacctct tggcaggcga ccttctggct gtcgaagagc
     4981 ccgcggaagc gcgccgaacc gaatgccggg cggccgttca ccaccaccag ctccacgtcg
     5041 cggatttccg cctgcacgat ggcgctgtag gggttcgccg acttcttcgc gaagagcgcg
     5101 aaatcggcga ggtagccggg cttgatgtgg ccgttcttct tgagcttgaa ggcatgggcg
     5161 gggttggacg tcaccatctt cacgatccgc tcgtccgaga gctcggcgcc gtaggtcttc
     5221 gtgtagtagt ccttgtcaaa cttcatctcg aagagcagat tgagtccgcc ggacatcggc
     5281 gagtcggtgc cgatgcagag gttcacgccc gcttcgagca tctttttcac gttggcggtg
     5341 gcgttgaaca tgaaggtgtt ggaatcgccg caccacacca ccgaggcgcc ctttttcttg
     5401 atggtctcga tgtcgctctc ggtgaaggcg atgccgtgca cgaggacgga gtgatcgccc
     5461 aggccgccgc gccgctcgag cgtcttcagg tcctgcttgg tctcgtcgtc gaaaccctcg
     5521 gcgatgtggg tgatgaacgg gatgtccttg tcgatcgcct tctggtattc cacggcgatc
     5581 tcgtcgcccc aggccagcgc gaaggacgcg accgaatgcg cgagcgcgta ctcgcggatc
     5641 gccttgaccg gcagcaggtc gaggaacggc tcgctcacga agtgcgggat gtggtccgag
     5701 acgatggtga cgcccgacac caggttccgg tacgccccga gaagatagag gtcgcggttc
     5761 tcgatctgct ggcgctcctc gtagacgagc gcgcttttga ggtcattgtc ccaggggagc
     5821 cagttctcgt acgggccatt gcccaccttg gggtagtagg tgcccaacag gtggtcgtgc
     5881 gcgttgatga ggccgggggc gaggaccgtg tccccggcgt cgaaggcgtc cacgccgcga
     5941 acgggaccgc cggagacgcg ctcgatcttg tcgccctgta tcgcgatccc ggcgtttttg
     6001 attgtctcgt ccggcgtcac gatggtcgcg ccggtcaggc cccatttccc cattatcgct
     6061 gtcccattat tcctgcgtcg gcgccggtcc gtcggcgctt cgccgcgctg atgtgacgta
     6121 attcggaatt ctgttcatcg ctacaataca tagtaaattg aacaaaccca gtcaagtaaa
     6181 atagccggga cgcggattca aaatttcacg ctctgaaaca gcgcgtcgat gtgctgcttg
     6241 aaaaagttgt agcggctctt cgtcgtgacg cccgcgatga tcacggggcc gcgcgcgccg
     6301 ttgacggtca tcgtttccat gacctgcgcg cggcggtcgc gcgacagcgt ccagtaatag
     6361 gcgcgcgatt cctgcgttcc ccagcggtcg ctgacggcgg tgttgcccat cgtgctcagc
     6421 cacaggtcgg tcgcgtcctc gggcgcggcg tcgagcgggg cggggcggct cacgtagaag
     6481 acgcccttgc cgtccggcgt cgcgaaggcg cggatcaccc ccttctcgat ctcggcgccg
     6541 atcggcgcgc gttcgacgac cgacgcgggg agcgtgagcg tgatgcccgc ctcggggacg
     6601 ctcaccgtcc gcgcgcccgc gagcttcccg gcgccgtcgc gccaggcctg gcggttgcgg
     6661 atcgcgcgaa ggtgctccac ggccttgcgg tcgatcgcgg cgacgttcga ttcgtccatg
     6721 cgccacgagg cgatccgcgc ggcgctctcg cgcgagagcg cgcgcgtgat ctcggccgtg
     6781 cgctccggcg tgatcatgtt cttcatggaa acgccccgct gcgcataggt cttcgcgagc
     6841 cggctcgcct gcgcaagccg cagcatgata tgtttcctca ggtccgactt gatgcggtgc
     6901 tcggcggtga tgcgctccgc gtcgaagtgc ggcacggccg ggatgacgga gcccgcctgc
     6961 agggcgcaat cgagcccggg atgctgcgcg ccgtcggacg cggcgttctg catcccggcg
     7021 aaggcgtcct ggtagcgggc gaaggcgtcg tccgcgtgcg tgatggcgcg gtatatcgcg
     7081 agcaggtcct gggcggattc catgagcgaa tcgatctcgc cgtgggcgag gcgctcgcgc
     7141 aggcgctgcc gcgcgcgggt cccgtcggcg gtcacctgcg cctgtttctg cgttaggtgt
     7201 ttcgccgcaa gacccgcgtg ggcgacgagg cgctttgcgt cctcgcgcga ctggctgcgc
     7261 gtctcccgat gccgcgtgag cgcggcgcgg tccaggaagc gacggtcggc ggcgtcgatc
     7321 gcgcccgctg cgtcgagccg tttcgcaagc gcggcgatcc gcttgttctg cgcgctcacc
     7381 agcgtgagcg acggcgcgag ggccgcctcc ggcagcgatt ggcgggcgcc caccagcgat
     7441 tcaagaaacg cgatggcggc gcggatcgcg atcgaccgtt tgtgcagttc gccctcgacc
     7501 gcggcgcggt gcgcggcgct tgtcgaagcc gacgggccca gcgcgaggcg ctgcatgcga
     7561 acgaggcgcg ccgcgacgtt ttccgctacg gtcgaggcgc cgatccgtgc cgatccctcg
     7621 atgtccatct gtgcgccgtg cgtcatggcg cgcgcggcga tgtgcgcgcg caccgagcga
     7681 tcgacggccg ggattgtttt acgctcaagc gccgcgatgg tctcgtcggc gcagcggccg
     7741 acgagggacg ccgtccgcgc gtcgtgttca gatatggcgc ggtccatgaa gagctcccag
     7801 gcgatgaggc gccactcctc ggcccggata tggtcgctca cgagccggcc gacggcgtcc
     7861 ggcgcaatgc cgggatcggc cgcgcggagg cgctcggcgc agcgctcgcc gacgatgcgt
     7921 cgcaggccgg gatcaggctc gatggggtcg tgccgggcga ggatgcggtc cagcgtgtgc
     7981 agggcgatga tgggcgggac gatctccgcc gcgagcgcgc ggacgcgggc gggggcgatg
     8041 ggcgcgtctg cgcgcgccga ggtcgcgccg cccgccgaac ggtcgaacgc cgcgcgcatc
     8101 gactcggcgc gcgtgatgtc gcggtccagc gcatcgcgca ggcgcaattc gagcggcgtg
     8161 cgcgccgtac gcgcggtacg ctccgcgcgc agctcgcgca cgcgatcatc ccaccacgcc
     8221 cgccggtcgt cgtccgttgc gcgcgccgct gccgtgggaa ccagcagcgc gagcatggca
     8281 atgacggcaa tggatgattt catggggcgc cccctgcgat acggcgtggc gatggattcg
     8341 tgctgcttat agtatcgtca gaaaaaaaag ttagataagc aaattccggg gggcccgagg
     8401 ggtgcggaac cgcgcctcag tcccgcgcga ggcgcttgag gatcaccagg gtcgcgtcgt
     8461 cgtcggggcg gtagagcgag agctgttcta tgattttttc gaggatggtt cgggtgtcgt
     8521 cgcgcccgtg cgccgcgagg atgcgcgcga gggcggcgcc gccgaactgg gcgcccagca
     8581 cgtccttcgc ctcgatgacg ccgtcggtgt agagtagcat aacgtcgccg ggatcgatct
     8641 ggaagctgcc gtcgcgggtg aactcgccga tctcgggacg gatgcccagc cacatgccct
     8701 cggtcggtat cgactccacg gcgccgtcgc gcgcccggta gacgaggatg tccaggtgca
     8761 gccccgcatg gagggccgcg ccgtccgcgt cgatggacag cgcggtaatg gtcatgtagt
     8821 tctcctcgtc catgcggcgt atgttgtggc tgatggcgcg gttggcgatg cgcaagagct
     8881 cgccgggcgg gacggtgggc cgctcgcgca ccaccgcctg gatgatcgac tcgaccatca
     8941 tcatgatgat cccggccgag acgccgtgcc ccgatacatc gccgatgacc acccagctcg
     9001 cggccggggt gttgatgatg tcgtaatagt cgccgcccac ctcctcggcc ggcttcatga
     9061 acgcgctcac gtcgtagccg gggatcgacg ggtgctgcgg gaggaggacc gtctggatct
     9121 tctccgccag gcgcatctcg ccccagagcg catcgcgcgc ccgctcggcc gtgcgcttcg
     9181 cggccttgat ctcgtcgcgc tggcgctgga actggtcggc caggtaggtg atgaacaacg
     9241 ccgcgggcag gaagcagagc gtgaagaaga tctcggtgaa cagcgcgccc ttctggcgca
     9301 gcacgacgat ggcgaaatag gtcccggcga tttgcaccat cgcggtcgcc agcgcgatcg
     9361 acagcgactg gagcaggttg gtgttcgaca gcgtgaagct cagggcgagg atcgcgaaga
     9421 agagccccgc gatccggatc tcgttgagcg ccaggatcca cagcaggtac aggacgagcc
     9481 agaaggcgaa ctggccgaag aagacggcgt tcgcgaaggc ggtggtgatc tcgcccttcc
     9541 gcgaggcgat gacggccaga aagacgatgc tgatgcccag cgacgcgccc gcgatggcgg
     9601 cgatctcccg gggcgagatg ctgctgatcc ccgtggccca ggcgacgagg ctcagggcct
     9661 gcgtgagcgc atagcagagg aacacgaaga gggtgtttcg catcatgatg cgccgtcgcc
     9721 gccggaactt gatgatgccg agtgccgtat ctgttttcat ggcgccagta ttccgcgtat
     9781 gggggtaaga tacacatggc cggggaaaat tgccatcgga aaattacact tgacggatgg
     9841 ggcgcgacga tgcagggtgc gggcccatga gacataatgc gagcaccgcg ccatgaccca
     9901 aaacgccgag acgcagctca tccccttcga ccggatgaag gaacaggtcg agcgcgccat
     9961 cgccgcgttc ctcgagcaga cccgctcgcc cgaggcggtc cccgcgccgc gcatcgcctt
    10021 cgagaagggg agcacgatca tcgcctttcc gctgccgttc ctcaacgagg cgctccttgc
    10081 ctcgttcatc ggcctcataa aaacgcacct ggcgaacgtg cgcattcacg ccttcggcga
    10141 cggctactac gccttccagt cgctcggcgg gaacctcttc cggaccgaga acatcctcga
    10201 caatgtcaag atcgagttct ccgggctcac cacgagtttc aaggccgaga tttcaaagaa
    10261 gggcggtctc acgcaggaag aggtgaacct tgcggtcgag ttgtacaagg aggcctattc
    10321 ggcggtcaag gatgatccca tcgcgcggct gcgcgcgatg ggcgcgtcgg tgtacaccga
    10381 caacggcgag cttacctggg cttatatcgc cggctatgac gaggtgaaga agaagatacg
    10441 ggagtccatc atcctgccgc tcaccaatcc ggagatgtac aactccatcg cgcgcctcac
    10501 gcgcaagacc ttcgagacca accggcccaa ggccatcctc ttcgagggct cgcccggcgt
    10561 ggggaagacc accgtcgcgc gcatcctggc cggcgaggtg tcggtgcccc tggtgtacgt
    10621 ccctatcgag tccatcatgt ccaagtggta cgggcagtcg tcgcagaacc tcgccgggat
    10681 cttcgacgcg gcggaggatc tgggcggctc gatcctcttt ctcgacgaga tcgacgcgct
    10741 cgcggggtcg cgcgaccaga acatgttcga ggcgacgcgt cgggtcctct cggtcctctt
    10801 gcgcaagctc gacggcatcg actcggtcgt gagcaccgtc accatcggcg ccaccaaccg
    10861 caagggcgac ctggaccacg cgctcatcag ccgtttcgat cagagcattt attttccgct
    10921 gcccaacgag cgcgagcgcg ccgcgatcct ggccaactac gccgcgcacc tctcggatga
    10981 ggagcgcgcg ggactggccg ggatcatcga tggcctctcc gggcgcacca tcaaggacat
    11041 ctgcgagatg gccgagcggc gctgggcgcg ccgcctcatc gtcaagggca tggagccgtc
    11101 gcccccgccc cccgagcact accggcagag cgcgcgcacc tggatcgagg acaaccgcgt
    11161 gcgggattga cggcgcgtgc gggtgatatc ttcgactgaa gcctcgattc gttacgcctc
    11221 cgaatcatcc cctcgagttc gattaatcgg ccgcatcacc agatagcgca cggtccctgc
    11281 gccggcgatc tcggcggcgc gcagcggcgg ccgggcgtgc cccttcgcgg cgaagtaggc
    11341 gcgcacggcc tcgtcctcca tgaaggcttc ggccagcgcg atctcgccgg ccccggcgaa
    11401 gggctggagc ttcgcggcca cgttcaccgg ctggccgaaa tagtcgatcg cgctgttgag
    11461 gttcaccgcc aggcacggcc cgcggttgat ggtcacgcgc gtgcgcaggg gcgcctcggg
    11521 atcgcgcccg tcgaaggtcg tgacgaaggc gcgtgcgcag cgcagcgcgt ccatcggatc
    11581 gtcgaaggag gccatgaccg catcgcccat cgtcttgatg atggcgccgc ggtgcgcggc
    11641 cgccagatcg tggctcgcgc ggaagaaggc gcgcacgatc gtgaaggccc gcgagtcccc
    11701 ctcgcggccg tacatgacgg tcgagcccac aatatcgacg aacatgatgt tctgcatgcc
    11761 cacgtcgatg gagaggtcag cggggaggga ctcgtcggcg aagaggtcgc ggaagtcctg
    11821 gaggttgaac aggtcgcgcg gccgcagggc gtcgcggtcg agctcgtgct cctcgacgac
    11881 gaaggtgcgc ggcgcggtcc cgggatttcg cataacgagc accgcgcccg acccggtttc
    11941 gaatgcggcc gccgtggcat cggcgtccca caggatctcg cggcgcggcg cgtcctcgcg
    12001 cacgtccagc aggtgataga tggttccgcc gaccgtgcgc aggcgatagc ggcccggggc
    12061 cagggcgagc ggaagcgcca gcaccgactc cgcgtccacc gcgcgctgga tgcggatatg
    12121 cggctttcgc gccggctcgg cgctgcagta gagcacctcc tccacgcggc ggatctccgg
    12181 gcgcggctga aaggtgatct cgacggcgtt caggctggag gtgtcgaagt cgacctggca
    12241 ggcgtcgcag cggcccttcg cgggcacctc ccacaggtgg ttgatccggg cgcgcacccc
    12301 gcggcagtgc gggcacacca cgtcccagtt catgctgagc atgccgcgcc gcgttccgcg
    12361 cagcatcgtc cccagggtgg cctcctcgcc ggtcgcgaag gtccgggcga gcgcgcgcgg
    12421 gcgcatgcga tgcagctcga catcgggggc cgccgcgatg tgcgcggcga gcttcacggt
    12481 ggtatccggg tcgcccccgt cggcgagcag cgcatgtttc gcgatggcaa ggcgctcgcc
    12541 gttggccgcc gttcccgtcg acgtctggcg caggtaatcg acgggctgaa gtatgattgc
    12601 gcccgcgagc gtccgttcgg ggaccagcgc ggcgaaggcg ttcgcgaagc gattcccgaa
    12661 ggacgtgcgc gcgccgatga gcaggaggcg cgcgggaaga tggcgcggga tccagccgaa
    12721 atacacctgc acgcgcgcgg tccccggcag agccggctcg atgtcgatca tgccgcgcag
    12781 gtagcgggcg atccccttcg aaaagatgcg cgtcatggtg atggagcgct ccgcctccca
    12841 ttcccagggc ggctcctccc actcctggcg catccccgcg aggcggtagc ttccgaagcg
    12901 cctgccgttt cgctcggtga aggacatgct cgggtagccc atgagctcgt taaggcgcga
    12961 ggtgtccgag acgatcggcc acagctccgc tggcgtcgc
//
LOCUS       DAORHF010000094        12890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_3945_length_12890_cov_5.179431, whole genome shotgun sequence.
ACCESSION   DAORHF010000094 DAORHF010000000
VERSION     DAORHF010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_12890_cov_5.179431"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="PKJ16_09375"
     CDS             <1..286
                     /locus_tag="PKJ16_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNV47240.1"
                     /translation="IHPALLPSFPGAHGQEQAFAYGVKISGCTAHFIDEGVDSGPVIM
                     QAAVPVMDDDTVDSLSARILKEEHRILPESVRLFCQGRLRVEGRKVFIKK"
     gene            393..953
                     /locus_tag="PKJ16_09380"
     CDS             393..953
                     /locus_tag="PKJ16_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47241.1"
                     /translation="MEALYIGIIYIVFLGFVVTMDFYINLFTNKDQFSKEELTGIFPH
                     LTATLGLIQAKYSTPIFLLFFALATFFGVLLPLISTHWFLNSAILFVIVFFVFSFIKD
                     HIDRIMVTASESFRDVVANVFSRHCEIFTLGFGVGTGASLIYVWGNNREMSFLWFLAN
                     MVIATIMIEFTLRTILKDTSTAPISG"
     gene            1223..1666
                     /locus_tag="PKJ16_09385"
     CDS             1223..1666
                     /locus_tag="PKJ16_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47242.1"
                     /translation="MKKFVMLALLAVVAISFTACGGGLGGQIKGEKFVTEGWVDDNTF
                     RTTATGVPKKGLTNKTQRRLTAREAALLYAQKTVLEKFKGARIEGASGMADGASTGIA
                     IAKEFGGLVRGGSVTKETYNDGDECEVVYEVAAPGLKKKVIGGAE"
     gene            1756..2214
                     /locus_tag="PKJ16_09390"
     CDS             1756..2214
                     /locus_tag="PKJ16_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47243.1"
                     /translation="MKALLLALIVCTLMISIGCGTTGAVKDDGAAVPADAAVIEGWIT
                     PEKFRVRAEGSPAPGITDPEQKKKSARDAAVENAQKLVIKNFVSTRVHSASGKLDIIN
                     TGIIITRNFGGTVRGGTVVQSRYDSAGRCTVYYEVTDKDLKKRVQTAGAQ"
     gene            complement(2291..2407)
                     /gene="rrf"
                     /locus_tag="PKJ16_09395"
     rRNA            complement(2291..2407)
                     /gene="rrf"
                     /locus_tag="PKJ16_09395"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            2514..3452
                     /locus_tag="PKJ16_09400"
     CDS             2514..3452
                     /locus_tag="PKJ16_09400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV47244.1"
                     /translation="MERIIETIIGATRDRPRLDRYLAERHTYLSRTAWRREIELGKIS
                     INGNTVRHAHRRLNAGDRVRYAGLNFEEPPVDADFAILYEDDDIIAINKTGNLPVHPA
                     GSFFRNTLLIMLEERYGKRFFPAHRLDRETSGVIVYAKTPDAATRLQANLRQARKRYV
                     AIVHGRMAPGRLTLDTPIGLDGESAVRTKRRAYPGAPESACTELRVLRSTERYSVVSA
                     TLRTGRLHQIRVHLRHAGYPVVGDKLYAHPDEVFLTFMRSGLTDELLNVLELPRSALH
                     ARKLSLIHPRTGRVMVFRAPLPDDMRSFIETRVRHG"
     gene            3445..3981
                     /locus_tag="PKJ16_09405"
     CDS             3445..3981
                     /locus_tag="PKJ16_09405"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HNV47245.1"
                     /translation="MAEPTVPPRAKLFIGVLTAREDLLLMTEERLVRRYGAIDFRTRK
                     LPFSHTDYYDSIGRNLFKVLYSFERLIPREDIVTVKLATNRLEKKITARTREKDAPRR
                     INIDPGYLTLSNVYLASCKEFFHRTYLWRGVYLENEYRYVGRRFQPWEWTYPDYRKME
                     YLDFFHQVRAIYRKQIEN"
     gene            complement(4015..4503)
                     /locus_tag="PKJ16_09410"
     CDS             complement(4015..4503)
                     /locus_tag="PKJ16_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47246.1"
                     /translation="MSRNMSYILSAVILLPILFILSGAHAEEPVLTDEIVTKYEATYP
                     AMWQVTQELDKTGKMPNSEEKDRKTQELLAKRAEILKENGWNDLWEYLDTMGRITRVF
                     VPLKVLAKFANRPEEDRKKAVAHVQNQLKGYSEEEIKTLMLHLPKLNEMLEKSGQIPK
                     DK"
     gene            complement(4633..6003)
                     /locus_tag="PKJ16_09415"
     CDS             complement(4633..6003)
                     /locus_tag="PKJ16_09415"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HNV47247.1"
                     /translation="MIHPERIQRLNEHKARRENFVLYWMQQSQRTTCNHALAHAIDLA
                     NGLSLPVIVYFGLTPLYPGANARHYTFLLQGLTVVRDALLRHGIPFIVRIEDPATGVV
                     EMARDAALAVTDRGYTRIQRTWRAEAARNMGCPLVQVESDVVVPVETASSKEEYTAAT
                     LRPKLKQQRDRFLVPLEEREIENRLGTGPDSLDVSDSEKVLARLGVDERVPAVNWISG
                     GQDEAEKWLEKFIKEKLKYYDTLRNDPSKDYLSHLSPYLHFGHISPLRIALRVMSSGH
                     TARHAFLEELIVRRELAMNFVHYNNMYDRYDSLPAWALKTLEEHAADRREYEYRRKEL
                     EEARTHDPYWNAAQRELLVKGKMHGYMRMYWGKKIIEWSASPRKAFDAALYLNDRYSL
                     DGCDPNGYAGVAWCFGKHDRPWREREIFGTVRYMNARGLERKFDIRAYARAVEEECGS
                     GAGDRR"
     gene            complement(6000..6554)
                     /locus_tag="PKJ16_09420"
     CDS             complement(6000..6554)
                     /locus_tag="PKJ16_09420"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HNV47248.1"
                     /translation="MGSGEFKGAQEAGMESDAGFRQVLRELLTGQTLAVLSTHCDGQP
                     YANLVAFAFSDDLRSLYFVTPRATRKHGNLQRDGRMALLITNGTNKETDFHAAMAVTV
                     IGSAGEIHGADRESALGHYVAKHPYLDDFARSPTCACMRVCVHTYILVNNFQHVMELH
                     LDNEPVPIKLMDLSDSRGGGVDRI"
     gene            6781..7011
                     /locus_tag="PKJ16_09425"
     CDS             6781..7011
                     /locus_tag="PKJ16_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47249.1"
                     /translation="MTDNLSCPRLTRKKKYAPSWKYGNEVFSIGLIRMLHRYQPVRYG
                     SARPRSTKKRVIDTAPGASLKAVIYGTKRRKE"
     gene            complement(7030..8205)
                     /locus_tag="PKJ16_09430"
     CDS             complement(7030..8205)
                     /locus_tag="PKJ16_09430"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47250.1"
                     /translation="MSALLVIAVSRATAAGGRAARWALIVALALGIACSGQKRSAKEK
                     PARTAKTDAAHGKPDRERRTPALSRDAHEDVVISLDDDARDVQVEKAELYTRLRSLFL
                     ANNIGQIERELKAAEKKYPLRDDRDYHYYWGVVQERAGKLDDAVKSYARAIELSPNYS
                     RARNALGKLYCRLKRYPEARVQYLLALEANPYNPFINYNLGSLYFEFNHLDVALKYLN
                     NAAEYKKNFGSAYHKIGIIMYRKGQYQNAVINLSMAITFNKASHITFYYLGMSQFALG
                     KHAEAVTSLTKSLGMKSDFFESAYELGKVNQLMGRYADALGYYRRAQRLRPDVSDINI
                     LIVDCLAELKRYSEAIKILREIVDKNPKNKLLAEKLKHLQERESIGNLTEPEDYYVY"
     gene            complement(8187..9608)
                     /locus_tag="PKJ16_09435"
     CDS             complement(8187..9608)
                     /locus_tag="PKJ16_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47251.1"
                     /translation="MPLIIGALALSLAAAPQKAPAAKTDAKTEAPPTTSQLADDEEGR
                     ESIARLVYGPNRWIALHFLLQPQGRFVGIYEENLETSRDGIIQKELYLKRCRIILNGH
                     VTKNISFFMETEDWEAGRSGSNANNGGYTTSSGYTSKDKGYDSTVTPADDYKHRHTVT
                     TTGTVKNNTFTQTAFINYRIFDELQFTVGLIPLPFTRQNLQSAASLLGVDYAAPFVDA
                     AIGGTTNNWRDMGAQVRGFLFGGIVDYKIGAFKGVSRSIERDENGAIIGITNPHDNPR
                     YSGRIQINFLDPEPGFFYSGNYLGKRKIISLGGGVDYQQSVYRKWYYDSETGERTNSD
                     LINYFAWTADVTVDYGFRGGNSFVFQGGYVGVYNNPATASLATDYYYNGSAFYVESGF
                     LFKRIVQPVLKYQSVFQRAVHADFKDRTYEEDRTIAHYIVGLNFFIREHNANIKMEYA
                     MPNGNNTSWSGQKWFIIQCQLFL"
     gene            9905..10993
                     /locus_tag="PKJ16_09440"
     CDS             9905..10993
                     /locus_tag="PKJ16_09440"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HNV47252.1"
                     /translation="MLAAAAIALLLCATWLGSCKKSAPPVRTAAEPPEAAEQGLTILF
                     GGDIMLDWGIKNAYRTHGAEYLLGGIKKELDRYDFRVANLECPIAYDGDPDPEKKYLF
                     LGDPADLPLLAYAGVNAVFLANNHSTDYGETALSDTIIHLRLNGIAVTGAGEDIETAR
                     RPVILRKHGISVALLGYSSIGAPSVHATVNRGGVAPAFLLHIKNDIQRIRAKVDYIVV
                     GLHWGREYSGYPERQQIALAHAIIESGADAIVGHHPHIYQGIEIHDGRPIIYSLGNFI
                     FGSINEDIRDNILVGITFERDGLHSLRIVPITRNNASRNAFQYRLLDSDAASAVFRHL
                     LDLSHPLGDDFPESAFQEGPFLHYSFDP"
     gene            complement(10975..11940)
                     /locus_tag="PKJ16_09445"
     CDS             complement(10975..11940)
                     /locus_tag="PKJ16_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNV47253.1"
                     /translation="MAKTAYLYDRLFMDHDTGWGHPEKPDRLPAIDERLKKEPFYTEL
                     VRIAPRKAASEHLELIHDRGYIERVRDEIAGGAHYLDSGDTVVCRKSFEVALYAAGGG
                     LAMCDAVMTGAAACGFCAVRPPGHHAERDQALGFCIFNNIAIAARYLQREHGVSRVAI
                     VDWDVHHGNGTQHSFDDDETVLYVSTHQYPHYPGTGAARETGTGTGKGYTLNLPMNPG
                     AGVDIFRAAFVERIVPALDEFRPEVVLISAGFDAHLSDMLSSIKLTAESFHEFTNMLL
                     GVADRHAQGRIIAFLEGGYDLTALGESVARMMRAFVEAEPYGSKE"
     gene            12131..12571
                     /locus_tag="PKJ16_09450"
     CDS             12131..12571
                     /locus_tag="PKJ16_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47254.1"
                     /translation="MIDIQISRTYQGTVAELFGAIDTVHKLAVVKPSLGRYRTARDEA
                     GLQIIDTALPYVFLRLSTRLRYTTNTEKYCELEQIKGSFSAYSCRYTFRASGASVALT
                     VRLMVSFPLGPIGFLLGLLFAPLEKFRLSRELSRIDARMRSARP"
     gene            12568..>12890
                     /locus_tag="PKJ16_09455"
     CDS             12568..>12890
                     /locus_tag="PKJ16_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNV47255.1"
                     /translation="MNHDKPLIVQSDNTLLLEVDNPLYEDARDAILPFAELEKSPEHI
                     HSYRVTPLSLWNAASSGMNAAGIIAALKKFSRYDIPEIVLASVKDVMGRYGLIRLEKR
                     GDELFL"
BASE COUNT         2525 a         4002 c         3731 g         2632 t
ORIGIN      
        1 catccatccg gccctgttgc cgtcctttcc gggcgcgcac ggccaggagc aggccttcgc
       61 ctacggcgtg aagataagcg gctgcaccgc ccacttcatc gacgagggcg tcgattcggg
      121 gcccgttata atgcaggccg ccgtcccggt gatggacgac gacaccgtcg acagcctttc
      181 ggcccgcatc ctcaaagagg agcaccgcat cctgcccgag tcggtccgtc tcttctgcca
      241 gggccggctg cgcgtcgagg gccggaaggt gttcataaaa aaataaaagt tcttgccatg
      301 acggtcgcgg cggcggcata cagtcccgta ttccgggcgc cgggtccgcg cgtcgtgcgg
      361 tcccgaagaa cgcgcaccgg gcgggtatgc acatggaagc gctctatatc ggcatcattt
      421 acatcgtgtt cctgggcttc gtcgtcacga tggattttta catcaacctg ttcaccaaca
      481 aggaccagtt ctcaaaagag gagctcaccg gcatcttccc gcacctcacc gcaacgctgg
      541 ggctgatcca ggcgaagtac tcgacgccga tctttctgct ttttttcgcg ctggccacgt
      601 tcttcggggt gctcttgccg ctgatttcga cgcactggtt cctcaattcc gcgatcctct
      661 tcgtgatcgt atttttcgtc ttctcgttca tcaaggacca tatcgaccgg atcatggtca
      721 ccgcctccga gagcttccgc gacgtggtcg cgaacgtctt ctcccgccac tgcgagatct
      781 tcacactcgg cttcggcgtt ggcaccggcg cgtcgctgat ctacgtctgg ggcaacaacc
      841 gcgagatgag cttcctgtgg ttcctcgcca acatggtcat cgccaccatc atgatcgaat
      901 ttacgctgcg aaccatcctc aaggacacca gtaccgcgcc gatcagcggc taaagccgct
      961 cggtgcgcga acgcgctatc cgggctggtt aacagaaatt cgaatatcgt cacaaaatag
     1021 acgcgggata aatattgaga ttttgcgttt ttttctgttg acttgcccct tctttgtctg
     1081 tatcgttcgt atcaatgaaa tcatagataa aatactatac gcggaatgca acatctcctg
     1141 cgaatggttt tatctatcaa aatgcgacgc gtacacgtga cgcggcgaac tattatcgaa
     1201 cacctttaag gaggagacgt ttatgaagaa gtttgttatg ctggcgctgt tggcagtcgt
     1261 tgcgatttct ttcacggcgt gcggcggcgg tcttggcggc caaatcaagg gcgaaaagtt
     1321 cgtaaccgaa ggttgggtgg atgacaatac cttccgcacc accgcgactg gcgttcccaa
     1381 gaagggcctc accaacaaga cccagagaag gctcaccgcc cgtgaagcgg cgctcctgta
     1441 cgcccagaag accgtcctcg agaaattcaa gggcgctcgc atcgaaggcg cgtccggcat
     1501 ggccgacggc gcttccaccg gtatcgccat cgcgaaggaa ttcggcggtc tcgttcgcgg
     1561 cggttcggta accaaggaaa cctacaatga cggcgatgag tgcgaagtcg tatacgaagt
     1621 cgcggctccc ggcctcaaga aaaaagttat cggcggcgcc gaataagtcc agaacgcaca
     1681 atggacgcac aagaagccgt ggcgtcgcca cggcttcttt tttacataca gcaccgttct
     1741 acagggagga cacccatgaa ggctttacta ttggcactta tcgtttgcac gcttatgatc
     1801 tctatcgggt gcggaaccac cggcgcggtc aaggacgatg gggcggccgt accggccgat
     1861 gccgccgtga tcgagggatg gatcacgccc gaaaaattcc gcgtacgcgc cgagggctct
     1921 cccgcgcccg gtatcaccga tcccgagcag aaaaagaagt cggcgcgcga tgccgcggtt
     1981 gaaaacgctc agaagctggt catcaagaac ttcgtctcga ccagggtgca cagcgcctcc
     2041 gggaagctcg acatcatcaa taccggcatc attataacga gaaatttcgg cgggacggtc
     2101 cgcggcggga cggtggtcca atcccgctat gacagcgcgg gccgctgcac cgtgtattac
     2161 gaggtgacgg acaaagacct taaaaaacga gtgcaaacgg ccggagcgca gtaaccggga
     2221 taatgctcac gatagaacgc aaaacgggcg ccagaaactg gcgcccgttt gatgtatggt
     2281 atgagtgcta aaccggcagc gtcctactct cccacacggt tacccatgca gtaccatcgg
     2341 cgatgaaggg cttaacttct gtgttcggaa tgggaacagg tgtgacccct tctccataac
     2401 caccggagat agcacttcag atgatcaccc tgtttttgtc aatggatttt ttcacttttt
     2461 ttcttgaaaa tttttttatc catgatttct gccctcaggg aatgcaaaca accatggagc
     2521 gaatcatcga gaccattatc ggcgcgacgc gcgatcggcc gcggcttgac cgctacctcg
     2581 cggaacgaca cacgtatctg agccggaccg catggcggcg cgagatcgag ttggggaaga
     2641 tatccatcaa cgggaacacc gtccgtcacg cgcatcgccg gctcaatgcc ggggaccgcg
     2701 tccgatacgc cggcctcaac ttcgaggaac cgccggtcga cgccgatttc gcgatactct
     2761 acgaggacga cgacatcatc gccatcaaca agaccggcaa tctgcccgtc caccccgcgg
     2821 gaagcttctt tcggaacacc ctgctgatca tgctcgagga gcgctacggg aagcggttct
     2881 ttccggcgca ccgcctggac cgcgagacat cgggcgtcat cgtctacgcg aaaactcccg
     2941 acgccgcgac ccggctgcag gcgaacctcc ggcaggcccg caagcgctat gtcgccatcg
     3001 tccacggccg catggccccg ggccgattga cgctcgacac gcccatcgga ctcgacggcg
     3061 agagcgccgt gcggaccaag cgccgcgcct atccgggcgc gcccgaatcg gcctgcaccg
     3121 agttgcgcgt gcttcgctcg acggagcgtt acagcgtcgt ctcggcaacc ctgcgtaccg
     3181 ggcggttgca ccagatacgg gtgcatcttc gccatgcggg ataccccgtg gtgggagaca
     3241 aactctacgc gcatcccgac gaggtcttcc tgaccttcat gcgttccggc cttacggatg
     3301 agcttctaaa cgtgctcgag ctcccgcgct cggccctgca cgcacgaaag ctatccttga
     3361 tccatccgcg cacagggcgc gtcatggtgt ttcgcgcgcc ccttccggac gacatgcgat
     3421 cattcatcga aacgcgagtg cgccatggct gagccgaccg tcccgccgcg cgcgaagctc
     3481 ttcatcggcg tcctcacggc gcgggaggac ctcctcctga tgaccgagga acgcctcgtc
     3541 cgccgatacg gcgccatcga cttccgcacg cgaaagctcc ccttttcgca cacggactac
     3601 tacgattcca tcggccgcaa tctcttcaag gtgctctact ccttcgagcg ccttatcccc
     3661 cgtgaggaca tcgtgaccgt gaagctggcg accaaccggc tcgagaagaa gatcacggcg
     3721 cgcactcgcg aaaaggacgc accgcgccgc atcaacatcg atccgggcta tctcacgctc
     3781 tccaacgtgt acctcgcctc gtgcaaggag ttcttccacc gcacctacct gtggcggggg
     3841 gtgtacctcg aaaacgaata ccggtacgtg ggacgccgct tccagccgtg ggaatggacc
     3901 tatccggact accgcaagat ggagtatttg gattttttcc atcaggtgcg ggcgatatac
     3961 cgaaagcaga tcgagaacta ggcgatatcg cgacaggcat gctgctgccg gcggctactt
     4021 gtccttgggg atttgccccg acttttcgag catctcattg agtttcggga ggtgaagcat
     4081 cagcgtcttt atctcttctt ccgaatatcc cttcaattga ttctggacat gggccaccgc
     4141 cttctttcgg tcctcttcgg gacgattggc gaatttcgcc agcaccttga gcggcacgaa
     4201 cacccgtgtg atgcggccca tggtgtcaag atactcccag aggtcgttcc agccgttttc
     4261 cttgagtatt tccgctcttt tcgcaagcag ctcctgggtc ttccggtcct tctcctccga
     4321 gttcggcatt tttccggttt tatcgagctc ttgcgtcacc tgccacatgg ccgggtaggt
     4381 ggcttcgtac ttggtaacga tctcatcggt gagcaccggc tcctcggcgt gcgctcccga
     4441 cagtataaaa agaattggaa gaagaatgac ggcactgagg atgtagctca tatttctaga
     4501 catagtgttc cctccttcat tcacgcaacg cgcccgatcc cgccccgact gttccaccat
     4561 ggcggcataa aaatgaaaaa gtcaataggt ttatttgtcg atcctccatg agagctcgga
     4621 catgcagact gatcagcgtc ggtcacccgc gccgctcccg cactcctcct ccacggcccg
     4681 ggcataggcc ctgatgtcga acttgcgctc aaggccccgg gcgttcatgt atcgcaccgt
     4741 gccgaagatc tccctttccc gccatggacg atcgtgtttt ccgaaacacc aggcaacgcc
     4801 cgcatacccg ttgggatcgc agccgtccag ggagtaccgg tcattgaggt aaagggcggc
     4861 atcaaatgcc ttacggggcg aggcgctcca ttcgatgatc ttctttcccc agtacatgcg
     4921 catgtacccg tgcatctttc ccttcaccag gagctccctc tgggccgcgt tccagtaggg
     4981 gtcgtgggtc cgcgcctctt ccaattcctt tctgcgatac tcatattccc tgcggtcagc
     5041 cgcatgctcc tcaagggtct tcagggccca ggcgggaagc gagtcatacc ggtcatacat
     5101 gttgttataa tggacaaaat tcatcgcaag ctcgcggcgc acaatgagtt cctccaggaa
     5161 ggcatgacgc gccgtatggc ccgaggacat gacccgcagg gctatgcgga ggggagagat
     5221 gtgcccgaag tgaagatacg ggctcagatg ggaaaggtaa tccttcgagg ggtcattacg
     5281 cagtgtgtca tagtatttca gcttttcttt tatgaatttc tccagccact tttccgcttc
     5341 atcctgtcct cccgagatcc agttgacggc aggaacgcgt tcatcgacac cgagccgcgc
     5401 gaggactttt tcgctgtccg atacatccag ggaatcgggg ccggtcccga gccggttctc
     5461 gatttcccgt tcttcaaggg gcacgagaaa gcggtcacgc tgttgtttga gtttgggcct
     5521 cagcgttgcc gccgtatatt cctccttgga agatgccgtc tcaacgggca caaccacatc
     5581 gctctccacc tgcaccagcg gacagcccat gttgcgtgcc gcctcagcgc gccaggtgcg
     5641 ctggatgcgc gtgtatcccc ggtccgtcac cgccagagcg gcgtcgcgcg ccatttcaac
     5701 cacccccgtt gccgggtcct cgatgcggac gataaaggga atgccgtgcc gcagaagagc
     5761 atccctgaca accgttaagc cctggagcaa gaaagtgtaa tgccgggcgt tcgcgccggg
     5821 atagaggggc gtgaggccaa aatacacgat gacgggtagc gagagtccgt tggccaagtc
     5881 tatcgcatgg gccagggcat ggttgcacgt tgtccgttgc gactgctgca tccagtagag
     5941 gacgaagttt tcccggcggg ctttatgctc gttcagtctc tgaattcgtt cgggatgtat
     6001 catatgcggt caacccctcc gccccgcgaa tcggacaggt ccatgagttt tatgggaacg
     6061 ggttcattgt cgaggtggag ttccatgaca tgctggaaat tatttaccag gatatacgtg
     6121 tgcacacaca cccgcataca cgcgcacgtc ggcgatcggg caaaatcgtc gaggtaagga
     6181 tgtttggcga catagtgacc gagcgccgac tccctgtccg cgccgtgaat ttcaccggcg
     6241 ctaccgatca cagtcaccgc catggctgcg tgaaaatcgg tttccttgtt cgtgccgttg
     6301 gtgatgagca atgccatgcg gccatcccgc tgcaggtttc cgtgctttcg ggtggcgcgc
     6361 ggtgtgacaa aatacagcga ccgcagatca tctgagaagg cgaatgccac cagattggca
     6421 tacggctggc cgtcgcaatg ggtcgaaagc accgcaagcg tctgtccagt caggagctcg
     6481 cgcagcacct ggcgaaaacc tgcatcagat tccatacctg cctcttgcgc gcctttgaat
     6541 tctcccgacc ccatgcacct gctcacgttt acgcccggat tgggcagtat gcctgttctc
     6601 gctcgtgtcc ccttccagaa caaggaccgg acagctcacg cggggcagca acggccatgg
     6661 agtgaatgag gctgtacgca gtgtgtacgc cgcggagtgc ccgatcgagt gactccgcgt
     6721 taaaggtgtt ccatcagtcg tcttccgata tagccggttg cgccggtgag gagtattttc
     6781 atgacagata atctatcgtg tcctcgattg acgcgcaaga aaaaatatgc gccctcttgg
     6841 aaatacggaa atgaggtgtt ctcaattggt ttaatccgga tgctgcaccg ctatcagccg
     6901 gttcgatacg gttcggctcg tccccgttcg accaaaaagc gcgtgatcga cacagcgccc
     6961 ggcgcctcgc tcaaagcagt gatttacgga acaaaacgcc ggaaggaatg aacgaggcgg
     7021 aggaacgcct cagtacacgt agtaatcctc gggctcggtg aggttgccga tcgattcgcg
     7081 ctcctgcaga tgtttcagtt tttcggccag cagcttgttc ttggggttct tgtccacgat
     7141 ctcgcgaagg atcttgatcg cctcggaata ccgtttgagc tcggcgaggc aatcgacgat
     7201 gaggatgttg atgtccgaga cgtccgggcg cagccgttgg gcgcgccggt aatacccgag
     7261 ggcgtcggcg tatctcccca tgagctggtt caccttcccc agctcgtagg ccgactcgaa
     7321 aaagtcgctc ttcatcccca gcgatttcgt aaggctggtg acggcctcgg cgtgcttgcc
     7381 cagcgcgaac tgcgacatcc cgagataata aaaggtgatg tgagaggcct tgttgaatgt
     7441 gatcgccatc gagaggttga tgacggcgtt ctggtactgt cccttccgat acatgatgat
     7501 gccgatctta tgataggcgc tgccgaagtt cttcttatac tcggcggcgt tgttcaggta
     7561 cttgagcgcg acatcgagat gattgaactc gaaatacagg ctccccagat tgtagttgat
     7621 gaagggatta tagggattgg cctccagggc cagcagatac tgcacgcgcg cctcgggata
     7681 gcgcttgagt cggcagtaca gcttgccgag cgcgttgcgc gcacgcgagt aattgggcga
     7741 gagctcgatg gcgcgcgcgt agctcttcac ggcgtcgtcg agcttgccgg cgcgctcctg
     7801 caccacgccc cagtagtagt ggtaatcacg atcgtcgcgg aggggatact tcttttcggc
     7861 ggccttgagt tcccgttcga tctgtccgat gttattggcc aggaagaggg agcgcagccg
     7921 cgtgtagagc tcggcctttt cgacctgcac gtcgcgcgca tcatcgtcga gcgagatgac
     7981 gacgtcctcg tgggcgtcgc gcgacaaggc gggggtgcgc cgctcacggt ccggctttcc
     8041 atgcgccgcg tcagtcttcg ctgttcgtgc gggcttctct ttcgcactcc gcttctggcc
     8101 cgaacaggcg atgccaagcg cgagcgctac aataagcgcc cagcgcgccg cgcgtccgcc
     8161 ggccgccgtg gcgcgcgaga cggcgattac aagaagagct gacactgtat gatgaaccat
     8221 ttctgccccg accaggaggt attgttcccg ttgggcatgg cgtattccat tttaatgttc
     8281 gcgttgtgct cccggatgaa gaagttaagc ccgacgatgt agtgggcgat cgtccggtcc
     8341 tcctcgtacg tccggtcctt gaagtccgcg tggaccgccc gctggaacac cgactggtac
     8401 ttgagcacgg gctgcacgat gcgcttgaac agaaagccgc tctcgacata gaaggcgctg
     8461 ccgttgtagt aataatcggt cgccagcgac gccgttgcgg gattgttgta cacgcccacg
     8521 tagccgccct gaaacacgaa cgaattgccg ccgcggaatc cgtaatcgac cgtaacgtcc
     8581 gccgtccacg cgaagtagtt gatgaggtcc gagttggtcc gctcgcccgt ctcgctgtca
     8641 tagtaccact tgcggtacac gctctgctgg tagtcgaccc cgccgcccag ggatatgatc
     8701 ttccgcttcc cgagataatt tccgctgtag aagaacccgg gctccgggtc caggaagttt
     8761 atctggatgc ggccggaata gcggggattg tcgtgcggat tggtgatccc gatgatcgcg
     8821 ccgttctcgt cgcgctcgat ggagcgggag acgcccttga acgcgccgat cttgtagtcg
     8881 acgatgccgc cgaagagaaa cccgcgcacc tgcgcgccca tgtcgcgcca gttattggtg
     8941 gtgccgccga tcgcggcgtc cacgaacggc gcggcgtaat cgacccccag gagcgaggcc
     9001 gccgattgca ggttctgtcg cgtgaagggc aacggaatga gcccgacggt gaactgcagc
     9061 tcgtcgaaga tgcgatagtt gatgaaggcg gtctgcgtga aggtattgtt cttcacggtg
     9121 ccggtcgtcg tcacggtgtg ccggtgtttg taatcgtcgg cgggcgtcac ggtcgaatca
     9181 taccccttgt ccttgctggt gtacccgctc gaggtcgtgt acccgccgtt atttgcgttg
     9241 ctgccgctgc gtccggcttc ccagtcctcg gtttccatga aaaagctgat gttcttggtc
     9301 acatggccgt tcaggatgat ccggcagcgc ttgagataga gctccttttg tatgataccg
     9361 tcgcgcgagg tctccaggtt ctcctcgtag atgcccacga agcgcccctg cggctgcagc
     9421 aggaaatgga gggcgatcca gcgattgggg ccgtagacca ggcgcgcgat gctctcgcgg
     9481 ccctcctcgt cgtcggcgag ctgggacgtc gtcgggggcg cctccgtctt cgcgtccgtc
     9541 ttcgcggcgg gggccttctg cggcgccgcg gcgagcgaca gggccagcgc gcctatgata
     9601 agcggcacag cgccgcatcg aatacacgtt ttcatcgcga aatcctcgta acgcccttcg
     9661 tgggtgatta aatgcgacaa actattcagg caagaatgta acggcaacca tattttatct
     9721 ttcgtcgccg ccacgacgag ccatatacga ctatgacgcc tcgtcggcga cgccggttac
     9781 acgccagaac aaaaattgtt gtctgaacaa taaaatattg ctactatgtc cccattcgtg
     9841 atcgtgaacg ccaatgaccc ggatatcgac aacaatgcca tcggccccct cgcgtcgcgc
     9901 gggaatgctc gcagcggccg ccatcgccct actgctgtgc gcgacatggc tgggctcctg
     9961 caaaaagagc gcgccgccgg tgcggaccgc cgccgagccg ccggaggccg ccgagcaggg
    10021 gctcacgatc ctcttcggcg gcgacatcat gctggactgg gggatcaaga acgcctatcg
    10081 gacgcacggg gccgagtacc tgctgggcgg catcaagaag gagctcgacc gctacgactt
    10141 ccgggttgcc aacctcgagt gccccatcgc ctacgatggc gatccggacc cggaaaagaa
    10201 atatctcttt ctgggcgatc cggccgacct gccgctgctc gcctatgccg gcgtcaacgc
    10261 cgtcttcctg gcgaacaacc actccaccga ctacggcgag accgcgctct ccgacaccat
    10321 catccatctt cggctcaacg gcatcgcggt gacgggtgcg ggggaggaca tcgaaaccgc
    10381 ccggcgcccg gtgatcctcc ggaagcacgg catcagcgtc gcgctcctgg gttattcgtc
    10441 catcggcgcg ccgtccgttc acgcgacggt caatcgcggc ggcgtcgccc cggcgttttt
    10501 gttgcacatc aaaaacgaca tccagcgcat ccgcgcgaag gtcgattaca tcgtcgtcgg
    10561 gctgcactgg gggcgcgaat attcagggta ccccgaacgc cagcagatcg ccctcgccca
    10621 cgccatcatc gaaagcggcg ccgatgcgat cgtcggccat cacccgcaca tctaccaggg
    10681 gatcgagatc cacgacggcc gcccgatcat ctactcgctg gggaacttca tcttcgggtc
    10741 catcaacgag gacatccggg acaatatcct ggtggggatc accttcgagc gcgacgggct
    10801 ccactcgctg cgcatcgtgc cgatcacgcg caacaatgcg agccggaacg ccttccagta
    10861 ccgtctgctc gacagcgacg ccgccagcgc ggtctttcgg cacctgctgg acctctcgca
    10921 ccccctgggt gacgacttcc ccgagagcgc gttccaggag gggccgtttc tccactactc
    10981 cttcgatccg tagggctcag cctcgacaaa ggcgcgcatc atgcgcgcga cgctttcgcc
    11041 gagcgccgtg agatcgtatc cgccctcgag aaaggcgatg atccggccct gcgcgtgccg
    11101 atcggcgacg cccagcagca tgttcgtgaa ctcgtggaag ctctccgccg taagcttgat
    11161 cgacgagagc atgtcggaca ggtgcgcgtc gaagccggcc gagatgagca cgacctcggg
    11221 ccggaactcg tccagcgccg gcacgatgcg ctcgacaaac gccgcgcgga agatgtcgac
    11281 ccccgcgccg ggattcatgg ggagattgag cgtgtagccc ttccccgtgc ccgtccccgt
    11341 ctcgcgcgcg gcgccggtgc cgggatagtg cggatactga tgggtgctca cgtacagcac
    11401 ggtttcgtcg tcatcgaagg aatgctgcgt cccgttcccg tgatgcacgt cccagtcgac
    11461 gatggccacg cgcgagacgc cgtgctcccg ctgcagatac cgcgcggcga tcgcgatatt
    11521 gttgaagatg caaaatccca gcgcctgatc gcgctcggcg tggtggcccg gcgggcgcac
    11581 cgcgcagaag ccgcaggccg ccgcgcccgt catcacggcg tcgcacatcg cgagcccacc
    11641 gcccgccgca tagagcgcga cctcgaagga ttttcggcac acgaccgtgt cccccgagtc
    11701 caggtagtgc gcgccgccgg cgatctcgtc gcgcacgcgc tcgatgtagc cgcggtcgtg
    11761 gatgagctcg aggtgctccg acgcggcctt tcgcggcgcg atccgcacga gctccgtgta
    11821 gaagggctcc ttcttgagac gctcgtcgat cgccgggagc cgatcgggct tttcgggatg
    11881 gccccatccc gtatcgtggt ccatgaacag ccggtcgtac agatatgcgg ttttggccat
    11941 gggaaccgtc cttggcatgg aatagtcagc acgccctatc aacactccgc cgctgtgaac
    12001 aattcaagaa gaaaaccgcc gttcgggcgt ataaatacgt caaaaaaagc ccggaaacgc
    12061 aaattcattt gacaaattgt gcacaggaat actatattca ccgactttgc ccgacaggcg
    12121 cgcacagccg atgatagaca tccagatttc aagaacatac caggggaccg tcgccgaact
    12181 cttcggggcc atcgacaccg tgcacaagct cgccgtcgtg aaaccgtcgc tcgggcgcta
    12241 tcgcacggcg cgcgacgagg cggggcttca gatcatcgac accgcgctgc cgtatgtgtt
    12301 cctgcggctg tccacgcgcc tgcgctacac gaccaatacc gaaaaatact gcgagctcga
    12361 gcaaatcaag ggcagcttct cggcctactc ctgccgctac acgttccgcg cctccggcgc
    12421 ctcggtcgcg cttacggtgc gcctcatggt gagcttcccc ctcgggccca tcggcttcct
    12481 gctggggctc ctgttcgcgc cgcttgaaaa atttcgcctg agccgcgagc tttcgcggat
    12541 cgacgcgcga atgaggtccg cgcgcccatg aaccacgaca agcccctcat cgtccagagc
    12601 gacaacaccc tcctgctgga ggtggacaac cccctctacg aggacgcgcg cgacgcgatc
    12661 ctcccgttcg ccgagctcga gaagagcccc gagcacatcc acagctaccg cgtcacgccg
    12721 ctgtcgctct ggaacgccgc ctcgtcgggg atgaacgccg cgggcatcat cgccgccctg
    12781 aagaaattct cgcgctacga catcccggag atcgtgctgg cgagcgtcaa ggacgtgatg
    12841 ggccgctacg gcctcatccg cctcgagaag cggggcgatg aactcttcct
//
LOCUS       DAORHF010000095        12643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4095_length_12643_cov_6.223070, whole genome shotgun sequence.
ACCESSION   DAORHF010000095 DAORHF010000000
VERSION     DAORHF010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12643
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4095_length_12643_cov_6.223070"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="PKJ16_09460"
     CDS             <1..559
                     /locus_tag="PKJ16_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV47256.1"
                     /translation="VKPGSAGKPVLNVETRLMDDDGNFVPVGTVGEIVHRSGQVMTGY
                     LNDPEKTAECFQFGWFHSGDLGVFDPDGYLTVVDRKKDMIKTGGENVASREVEEVLYQ
                     HPAVEEVAVIGLPDPKWIEIVAAVVVPKKDMNVTEKEIIAFAKDKLAGFKCPKKIVVR
                     DKLPKNPSGKILKRELKAELANAAG"
     gene            complement(556..1077)
                     /locus_tag="PKJ16_09465"
     CDS             complement(556..1077)
                     /locus_tag="PKJ16_09465"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV47257.1"
                     /translation="MTHQHDFDKAHFRALPNRDNHKCFGCSPDNPHGLRMTFHTDEKS
                     VVSWVTIPEHLCGWNDLAHGGVISTVLDEIMSWAAIYLLRHLILTKSMTVEFRKPVFV
                     GKEVVAQGTVVEHPNSREAIMAGALYQDGRLCAKSTGSFALFTPEAIRKRGLLDGDVI
                     DAMEALFRKDAAR"
     gene            complement(1345..2271)
                     /locus_tag="PKJ16_09470"
     CDS             complement(1345..2271)
                     /locus_tag="PKJ16_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47258.1"
                     /translation="MASLVIVASLSCASAEKREGFAAGVYTGRAEGGSLIYRNDVKRA
                     RENAIAAALERLVEKAKAATPPPAGPAAADAFVLGRNARIVKNMRIIDEGVKGAHFVA
                     TVEGEVHPASAYGEVRALIRNYRIPRVMIFVGEEIEGARSRPGLTLTELAIGGTLVSL
                     GFDVLDLGAAQVRAPERIGDDLQAAPGGSSAAKKLRAMGVGLVIVGMAKTIDQSKAMS
                     QFKVAMRSKQAIIGLKAIDLATGAVMASVSVNAPGNGLDDAVASRNAVENCVARALGR
                     FNLREERFQPGAFMRQLILSYARMSERTLTQR"
     gene            2450..3664
                     /locus_tag="PKJ16_09475"
     CDS             2450..3664
                     /locus_tag="PKJ16_09475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV47259.1"
                     /translation="MNLSQPVAALLIALLFAASAPDARAADWRQEYCHGGATCMLLEQ
                     NGSTVDMYLRNVFPSDEIVTTMSFRQTAPMENLRTDATLPFTRVIAGTAPVKVLTFTI
                     IDTTKRWAPNVTYYWQYGSIAAKHDAARPYELPFERGKRFRVGQAFNGKITHHGEFAY
                     SVDFLMPIGTPIHAAREGVVIAVEGRYGEGKLTPAYKEKNNYVFILHPDGTIGRYVHM
                     VKDGISVKVGQHVKAGDRIATSGNSGYSDSPHLHFDVARVLDERRSTTVPFAFRTDYA
                     NAEEPREALWYSFSGDGAHKVRVPVEPSDVQLCRRVVNNEFRECGNEFAAGSKVLVAL
                     PLGVPGVYRVKTVMRKTGAPPIVSDFRTHKDWWYTYATAELPGAHSAAGEWIVEVFID
                     GKKIREIPFRVR"
     gene            complement(3697..5118)
                     /locus_tag="PKJ16_09480"
     CDS             complement(3697..5118)
                     /locus_tag="PKJ16_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47260.1"
                     /translation="MIRPARLRAASFLIAAWALSCSSFPETLPPDRFSIDGANRAGLY
                     TEIERVAVGPDGGIALLARIDLRDDTTSSPRTHLAPARLTADGGIDASFAVDPFFART
                     RAYQPHFLFAGGDGGIVFSAIVRACDATGKFTGDRPTLFTLSPDGSIRDMRVIAPARF
                     HLEHIHDIVPVGAGGTLGGLYVVARLIPKARGTQSERATAWTGVIRLLANGDEDAAFE
                     PCLRDERTILRTAPTAVMRDGGIALWAYRDEYNKSFEIIRLTARGRADPSFRRGAYRI
                     ALGGTATDIGALAVDTRGRLLLAVTTASDDARRDRANHILRLRPDGGPDTSFIHRVAS
                     GTADVLPDLVPHENGSVSYLTRDYAPQTAYRIRLHRIDGSGVADATFSDATAPRRSEQ
                     RSPWRRTLIAGSRGSVVIADRHIEMVQPTGKTRFIARVCSDFDFTPNATAYERARDGC
                     WNAIAYAGFRFLELFGWKMKTMP"
     gene            complement(5127..6086)
                     /locus_tag="PKJ16_09485"
     CDS             complement(5127..6086)
                     /locus_tag="PKJ16_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HNV47261.1"
                     /translation="MKLLRFRRTETSRPEWGIVDDGAVRPIVARHTVAEIFTAVKSGK
                     IPTARPLRLDAVIMLPPTDENVHLYCAGLNYRDHAKELRMPVPKSPVFFTKSVGALCG
                     ARDDIVYPSGTRLLDYEVELALIVGRRIGLSDVLRPDTLKDFVLGLAVMNDVSARDVQ
                     LSATQWFLGKSFRSFAPLGPWVQTLDEDVMQRLRDLRLELRVTAPDGTTYPNKCQSGS
                     AADMIFPPHELLNCLREKFDLLPGDVIATGTPRGVAMSRPGRFKTRIAEILGIAPGKR
                     VATYIAGEIKNNRNYLERGDVVSARIATDNGFVDLGEQRTRVV"
     gene            complement(6293..7057)
                     /locus_tag="PKJ16_09490"
     CDS             complement(6293..7057)
                     /locus_tag="PKJ16_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HNV47262.1"
                     /translation="MPQTLRPDNYARQTLERYTGCEIGEFGPWIVLVNFPRYLEDFAR
                     MHDVPVKSAYWSAAHDPKSGVSVINFGVGSPSAGIITNCLSYLDHIKAVLMLGMCGGI
                     ADTLEVGDIVLPTASVRDEGTSKHYLPIDVPAMPHTRILRLSNEVIRGEPGARVAAGL
                     MLTTDYRMWEFDDEFIEYINKHRIIAIDMEIATIFSVAYALDMPAGAIMLVSDMPLRK
                     GGIKGKEHMHAVFEKHTEHHLHMGLKTIEELKRVGL"
     gene            complement(7210..9039)
                     /locus_tag="PKJ16_09495"
     CDS             complement(7210..9039)
                     /locus_tag="PKJ16_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47263.1"
                     /translation="MKPIRQSIITLFIAAAIAIPSHSATRRMNILVYPFENLGAKELA
                     WISAGMTDTVVSDLQRVKSVNVITEEGRKKAVEELELAQAGLFGDARIAKVGSLAGAD
                     VIFTGTYQRSGRLIRVNARLVTVATGVIEKSIKIDGTIDGIFDLQDKIVLGLLAETKN
                     APAGVEPPRLDDAARADITGKKRPALTAYEYYAKGLAVRDANPNEALALFEKAVAADP
                     AYAEALVRAGYTAGVILNRFDRAISYLTRAEKTLAGRGESNAPAFASLLNTMGTVADS
                     RGDLARALEYFNRSKALRDRLGLARTSGYAALVANIGNIFYRQERHADALAQYNAALA
                     LMDALGLSGTADYAMLLMNIGNCLKDTGRLGEAIARYSKALAHFESRGIRNVSYANVL
                     ANMGLALQQSGTADPALDAFRKARDIRDRLSLRNTVEYAYLLNNIGITHHGRGDLKQA
                     GDNYRAAQALYETLGQMKTKDYADLLVNMGHLAGDNKQLDAALENYLKAKAIRESLNL
                     TRNPIYANLLLNIGSAYYQKRNFAAAIEAMERSRATLESIGRTTGESYGGVLFNLAVI
                     YEAAGQKAKAGAFYRQSYDIMAKAGVKGEILERARTGARRLGK"
     gene            complement(9199..9465)
                     /locus_tag="PKJ16_09500"
     CDS             complement(9199..9465)
                     /locus_tag="PKJ16_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47264.1"
                     /translation="MKKMAIALSVVVIAGALTAVGAQTVEQKQAKIREQGMQECWKMG
                     ALKKGFYQRPCTKMTNAAEKKKCEADAIKKAETEQKECEKKFSK"
     gene            complement(9625..11766)
                     /locus_tag="PKJ16_09505"
     CDS             complement(9625..11766)
                     /locus_tag="PKJ16_09505"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47265.1"
                     /translation="MPTKRQSHTTGRHRRNHSFLLILPVVFLISTITAFAAEPITLDA
                     DALRGTPIGLHVEYLRDPGGTRSHDEILRMDRLGAAQWTAAKSKSLSFGYITDAVWIK
                     FSATNPEPKEKEWYLEVGYSHLDHVDLYIPTGDGGYRVKREGDSKPFYDREYRYRNFI
                     FPVLQRPGTQTYYLRVTSTGAIRIPLTIWSRQVLAENSNNESLFFGLLYGILLIMIAY
                     NFFVFLSIRDRDFLWYLLFVTAGLIFSSTLNGTGFQYLWPQHPWMNNNFIISVHCCSI
                     FLYLFFRSFLNIRDLSPTINRFIILMTGIHCAAILLSLLVPYRYSVRFTAFSGILCVS
                     IVLLISLYCLLRRVQFAQYFAMAFLPLLVLLFPTGLTYIGVIPGAFLLEWGADIGLIS
                     MMVFFSFTLGKKLNTLQLERISAQAETIRIQQDATATLERKVEERTGELAAAMEEVTR
                     VNERLMKSNASLEMRNRTIENEIALARKIQNSLIPLRTPAPYISAYYKPMETVGGDFY
                     DFIAVPGSERVGVFLSDVSGHGVPAAFITSMIKTILLQAGDRLADPAGLLGYMNDVLR
                     GQTAGNFVTAFYGIYDPRARSLLYSNAGHNRPYLLSSDRVIQLQGSTGIAVAVFSNDK
                     LMRANKRFENRTETLEPGSKLLLYTDGLIESRPVGGGAFFMYASLERILRDNAHVPCD
                     GFLAELMTELRAFRQSESFEDDICLICLDVV"
     gene            complement(11874..>12643)
                     /locus_tag="PKJ16_09510"
     CDS             complement(11874..>12643)
                     /locus_tag="PKJ16_09510"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HNV47266.1"
                     /translation="PAVMAAAAARLAEQGCRAIDINMGCSAPDIMKTGGGAALLADPP
                     RAREIVRACRSAAPCGLTVKMRSGYERTDLPALLDFARMLQDEGVNAITLHGRHAKLG
                     FRRTADWSAVRAVKERLSIPVIGNGDIDAAETAVRRLRESGCDGVMIGRAAVQAPWIF
                     ALCDALVGGGHVVRDIDIEAVFVDVLEGIRDFLEEKYHRSRGHRFCFYFCQNVHFSHE
                     LFRTIRRESAIDAMIALVRGYFARNPHERVKTVRAGA"
BASE COUNT         2194 a         3908 c         4178 g         2363 t
ORIGIN      
        1 cgtgaagccc ggctcggccg ggaagcccgt cctcaacgtg gagacgcgcc tcatggacga
       61 tgacgggaac ttcgtcccgg tagggacggt gggcgagatc gtgcaccgct ccggccaggt
      121 gatgacgggc tatctcaacg atcccgagaa gaccgccgag tgcttccagt tcggctggtt
      181 ccacagcggc gacctgggcg tgttcgatcc cgacggctac ctcacggtgg tggaccgcaa
      241 gaaggacatg atcaagaccg gcggcgagaa cgtggcctcg cgcgaggtgg aggaggtgct
      301 ctatcaacac cccgccgttg aggaggtcgc ggtgatcgga ttgcccgacc cgaagtggat
      361 cgagatcgtc gcggcggtgg tggtgcccaa gaaggacatg aacgtcacgg agaaagagat
      421 catcgcattc gcgaaggaca agctcgcggg gttcaagtgc cccaagaaaa tcgtcgtgcg
      481 cgacaagctg cccaagaacc cctcgggcaa gatcctcaag cgcgagctca aggccgagct
      541 ggcgaacgcg gcgggctagc gcgccgcgtc ttttcggaag agcgcctcca tcgcgtcgat
      601 cacgtcgccg tcgaggaggc cgcgcttccg gatcgcctcg ggcgtgaaaa gcgcgaagga
      661 ccccgtcgat ttcgcgcaga ggcgtccgtc ctgatacagc gcgccggcca tgatcgcctc
      721 gcggctgttc gggtgctcca ccaccgtccc ctgcgcgacg acctccttcc cgacgaatac
      781 cggcttcctg aattcgaccg tcatgctctt ggtgaggatg aggtgccgga ggaggtagat
      841 cgccgcccag ctcatgatct cgtcgaggac ggtcgagatc acgccgccgt gcgccaggtc
      901 gttccacccg cacaggtgct cggggatcgt gacccacgag accaccgatt tctcgtcggt
      961 gtgaaacgtc atgcgcaggc cgtgcggatt gtcggggctg cagccgaagc atttgtggtt
     1021 gtcgcggttg gggagcgctc ggaagtgcgc tttgtcgaaa tcgtgttgat gggtcatggc
     1081 cggtatccta tgcgaaatat ccaattggcg tattgtcaaa cacgctgtca ttgcgggcga
     1141 agcgaagcac tccgttcaca cgtgcgccag cgtatcgctg tcctgcggta tgcactataa
     1201 cattttcgag agcacacaga tcgcttcgtc gctagcgctc ctcgcaatga catggaaacg
     1261 gttttgcttc aactcaaatg agaatcaccc catcaggcta ccggtatgga ttttgtcaag
     1321 aagagcgcac cggagcgcga tgaatcagcg ctgcgtgagc gtccgttcgc tcatgcgcgc
     1381 gtagctgaga atgagttgcc gcatgaaggc gccgggctgg aaccgctcct cgcggagatt
     1441 gaaccgtccc agcgcgcggg cgacgcagtt ttccacggcg tttcgggagg cgacggcgtc
     1501 atcgagccca ttgcccggcg cgttgacgct caccgaagcc atgacggctc cggtcgcgag
     1561 atcgatggcc ttgaggccga tgatcgcctg cttcgatcgc atggcgacct tgaactggga
     1621 catcgccttc gattggtcga tcgtttttgc catgcccacg atgacgagcc ccacgcccat
     1681 ggcgcggagt ttcttcgctg cggacgatcc gccgggcgcg gcctggagat cgtcgccgat
     1741 gcgttcgggc gcacggacct gcgccgcgcc gaggtcgagc acgtcgaacc cgagcgagac
     1801 gagcgttccg ccgatcgcga gctcggtgag cgtgagcccg gggcggctgc gggcgccctc
     1861 gatctcttcg ccgacgaaga tcatcactcg cggaatgcgg tagttccgta tgagcgcacg
     1921 cacctcgccg taggcggacg ccggatggac ctcgccttca acggtcgcga cgaaatgcgc
     1981 gcccttcacg ccctcgtcga tgatcctcat gttcttgacg atccgtgcgt tgcggccgag
     2041 cacgaaggcg tcggcggccg cgggccccgc ggggggcggc gtagcggcct tcgccttttc
     2101 cacgagacgc tcgagcgctg cggcgatcgc gttttcgcgc gcccgcttca cgtcgttgcg
     2161 gtagatgagg ctcccgccct ccgcgcgtcc ggtatagacc cccgccgcga agccctcccg
     2221 cttttccgcg gatgcgcatg agagcgatgc gacgattacg agtgacgcca cagcggcgcg
     2281 cgccgtgcgc gccgcaatcg gccctaaaaa tacttgtcgt ggtatcgcgt gcatggtagt
     2341 gtgtggtgat ccggcgccgc gtcccgtcgc gcgttgcagg cgggacgact gtggactcgt
     2401 gtaccgcttc gggcgctgcg cgtcaagtac gaatatcgtg gaggaagcca tgaacctgtc
     2461 ccagcccgtc gccgcattgt tgattgcgct gctgttcgcc gcgagcgccc ctgacgcccg
     2521 cgcggccgac tggcggcagg agtactgcca cggcggcgcc acctgcatgc ttctggagca
     2581 aaacggatcg accgtggaca tgtatctgcg gaacgttttt ccctctgacg agatcgtaac
     2641 gacgatgtcg tttcggcaaa cggcgccgat ggagaacctg cgcaccgacg cgacgctccc
     2701 gttcacccgc gtgattgcgg gaaccgcgcc cgtcaaggtg ctcacgttca cgattatcga
     2761 cacgacgaaa cgctgggccc cgaacgtgac gtactactgg cagtacggat cgatcgcggc
     2821 gaagcacgat gcggcgcggc cctacgagtt gcccttcgag cgcgggaagc gctttcgcgt
     2881 tggccaggcc ttcaatggca agatcacgca tcacggtgag ttcgcctact ccgtggattt
     2941 cctcatgccc atcgggacgc ccatccacgc ggcgcgcgag ggcgtggtga tcgccgtcga
     3001 gggccgctac ggcgagggaa agctcacgcc cgcatacaaa gagaagaaca attacgtgtt
     3061 cattctccat cccgacggca ccatcgggcg ctatgtgcac atggtgaagg acggtatatc
     3121 cgtgaaggtg ggtcagcacg tgaaagcggg cgatcgtatc gccacgtcgg ggaacagcgg
     3181 gtacagcgac tccccccatc ttcattttga tgtcgcgcgc gtgctcgacg agcggcgctc
     3241 gacgacggtg ccgttcgctt tccgcaccga ctacgcgaat gccgaggagc cgcgcgaggc
     3301 gctctggtac tcgttttctg gcgatggggc gcacaaggtg cgcgttcccg tggagccctc
     3361 ggacgtgcag ctgtgccggc gggtcgtgaa caatgagttc cgtgagtgcg gcaacgagtt
     3421 cgcggcggga tcgaaggtcc tcgtggcgct tcccctcggc gtgccgggcg tgtatcgcgt
     3481 taagaccgtt atgcgcaaga cgggcgcgcc gccgatcgtc tccgatttcc gcacgcacaa
     3541 ggactggtgg tacacctacg ccacggctga gctcccgggg gcgcacagcg ccgcgggcga
     3601 gtggatcgtc gaggtgttta tcgacgggaa aaaaatccgg gagatcccct tccgcgtcag
     3661 gtgatccgca cggcaccgcg cacgtcgatt gtgtcgttac ggcatcgttt tcattttcca
     3721 gccgaacaat tccaggaagc gaaacccggc gtatgcgatg gcgttccagc agccgtcgcg
     3781 cgcgcgttca tacgccgttg cgttgggcgt gaaatcaaag tcgctgcaga ccctcgcgat
     3841 aaagcgcgtt ttccccgtcg gttggaccat ctcgatgtgc cggtccgcaa tgacgaccga
     3901 cccgcgcgac cccgcgatga gcgttctgcg ccatggcgag cgttgctcgg agcgccgcgg
     3961 cgcggttgca tcggaaaacg tggcgtccgc aacgccggag ccgtcgattc gatggagccg
     4021 gatgcgatag gcggtctgcg gtgcgtaatc gcgcgtcaga tatgacaccg acccgttttc
     4081 atggggcacc aggtccggca gcacgtcggc cgtaccggag gcgacccgat gtatgaatga
     4141 cgtgtcgggc ccgccgtccg ggcgcagccg caggatgtga ttggcccggt cgcgtctcgc
     4201 gtcgtcggat gccgtcgtca ccgccagaag caaccgtccg cgcgtatcga ccgccagggc
     4261 gccgatatcc gtcgccgtcc cgccgagcgc gatgcgatac gcgcctctcc ggaaggacgg
     4321 atccgctcgc ccgcgcgccg tgagccgaat gatctcgaag gatttattgt attcgtcccg
     4381 gtacgcccag agcgcgattc cgccatcgcg cataacggcg gtcggcgccg tccggagaat
     4441 ggtccgctcg tcgcgaagac agggctcgaa ggcggcgtcc tcgtcgccgt tcgcgagaag
     4501 ccggatgacg ccggtccagg ctgtcgcgcg ttcggactgc gtcccgcgcg ccttcgggat
     4561 cagccgcgcg acgacatata atccgcccag cgttcctccg gcgccgacgg ggacaatgtc
     4621 gtggatatgc tcgaggtgga agcgggccgg ggcgatgacg cgcatgtcgc gaatcgatcc
     4681 gtcgggcgag agcgtgaaga gggtggggcg atcgcccgtg aatttccccg tcgcatcgca
     4741 ggcgcgcacg atcgcggaaa agacgatgcc gccgtccccg cccgcgaaca ggaagtgggg
     4801 ttgatacgcc cgggtccggg caaagaacgg gtcgacggcg aacgacgcat cgatgccgcc
     4861 gtccgccgtg aggcgcgccg gggcaaggtg cgtgcgcggg ctcgatgttg tatcgtcgcg
     4921 aaggtcgatg cgggccagga gggcgatccc gccgtcgggc ccaacggcga cccgttcgat
     4981 ttcggtgtag agcccggccc ggttcgctcc gtcgatcgag aagcgatccg ggggcagcgt
     5041 ttcgggaaag gaggagcagg agagcgccca tgccgcaatg aggaacgatg cggctcgcag
     5101 acgcgccggc cggatcatcg cgcgcctcac accacgcggg tgcgctgctc gccgaggtcc
     5161 acgaagccgt tgtcggtggc gatgcgcgcg ctcacgacat cgccgcgttc gaggtagttg
     5221 cggttgttct tgatctcgcc cgcgatgtac gttgcgacgc gcttccccgg cgcgatcccg
     5281 aggatctccg cgatgcgagt cttgaaacgt cccggccgcg acatcgctac gccgcgcggc
     5341 gttcccgtgg caatgacatc gccggggagc aggtcgaact tctcgcgcag gcagttgaga
     5401 agctcgtgcg gcgggaagat catgtccgcc gcggaacccg actggcattt gttcggatac
     5461 gtcgtcccgt ccggcgccgt tacgcgaagc tcgagtcgca ggtcccggag gcgttgcatg
     5521 acgtcctcgt ccagcgtttg cacccagggg cccagcggcg cgaaggagcg aaagctcttg
     5581 cccaggaacc actgggtggc cgaaagctgc acgtcgcgcg ccgagacgtc gttcatgacg
     5641 gcaaggccca gcacgaagtc cttcagggtg tcgggccgga gaacgtcgct tagtccgatc
     5701 cgccgcccga cgatcagggc gagctccacc tcgtagtcca ggagccgcgt gcccgacgga
     5761 tagacgatat cgtcccgcgc cccgcagagc gcgccgaccg acttggtgaa aaagaccggg
     5821 ctcttcggga cgggcatgcg aagctccttc gcgtggtccc ggtagttgag ccccgcgcag
     5881 tacaggtgga cgttctcgtc cgtgggcggc agcatgatga cggcatcgag ccgcagcggg
     5941 cgcgccgtag ggatcttacc gcttttgact gccgtgaaga tttccgcgac cgtgtgccgc
     6001 gcgacgatgg gacgcacggc cccgtcgtcg acgatccccc attcggggcg cgatgtttcg
     6061 gttcgtctga atcggagcag tttcattaat ggatacccgt aaagtggaaa aatattaaac
     6121 acgggcaggt actatcgtga cctcaccccg cctccggcgc gcccttagcg acaggatgtc
     6181 gcgtatgcag catcgtacat ggatgtagga gatgctgcct cccgcaggag agggctttga
     6241 catacgtaac ttttgcctta caccccctct cctttgggag agggggtgag gtctacaacc
     6301 ccacccgctt gagctcctcg atggttttga ggcccatgtg caggtggtgc tccgtgtgct
     6361 tttcgaacac cgcgtgcata tgctccttgc ccttgatgcc gcccttccgc agcggcatgt
     6421 cgctgaccag catgatggcg ccggccggca tgtcaagcgc atacgccacg gagaatatcg
     6481 tcgcgatctc catgtcgatg gcgatgatgc ggtgcttgtt gatgtactcg atgaattcgt
     6541 cgtcgaactc ccacatgcgg tagtcggtgg tgagcatgag gcccgcggcc acgcgcgcgc
     6601 cgggctcgcc gcggatcacc tcgttggaga ggcgcagaat gcgcgtgtgc ggcatggccg
     6661 gcacgtcgat gggcagatag tgcttgctgg tgccctcgtc gcgcacgctc gcggtgggga
     6721 gcacgatgtc gccgacctcg agggtgtcgg cgatcccgcc gcacatcccc agcatcagca
     6781 ccgccttgat gtggtccaga tacgacaggc agttggtgat gatgcccgcc gacggcgagc
     6841 ccacgccgaa gttgatcacc gagaccccgc ttttcgggtc gtgcgcggcc gaccagtagg
     6901 ccgacttcac ggggacatcg tgcatccgcg cgaagtcctc aaggtagcgc gggaagttga
     6961 ccaggacgat ccacgggcca aactcgccga tctcgcagcc ggtatagcgc tcgagcgtct
     7021 ggcgcgcgta gttgtcggga cgcagcgtct gtggcatggg cggtggctcc tcgttcgctc
     7081 gtgaaggttc cggtgcggcg gtcatcagaa tgcgactgac ggttggattt tgtcaagcag
     7141 agtgtggaag cgggggcgaa gcagtgccga gggggggtga aataatcatg aaaaatgcat
     7201 gaccccgcct cactttccca gacgcctcgc ccccgtgcgg gcccgctcga ggatttcgcc
     7261 cttcacgccg gccttcgcca tgatgtcata gctctgccgg tagaacgctc ccgccttcgc
     7321 cttctggccc gccgcctcgt agatcaccgc cagattgaac aagaccccgc cgtaggattc
     7381 gcccgtcgtg cgtccgatcg actcgagcgt cgcccgtgag cgctccatcg cctcgatggc
     7441 ggccgcgaag ttgcgcttct ggtagtaggc gctgccgata ttgagcagca ggtttgcgta
     7501 gatcggattt cgcgtgaggt tcagcgattc gcgaatcgcc ttcgccttca ggtagttctc
     7561 gagcgcggcg tcgagctgct tgttgtcgcc ggcgagatgc cccatgttga ccagcagatc
     7621 ggcgtagtcc ttcgtcttca tctgccccag cgtttcgtag agggcctgcg cagcgcggta
     7681 gttgtcgccc gcctgcttca ggtcgccgcg gccgtgatgc gtaatgccga tattgttcag
     7741 gagatacgcg tactcgacgg tgtttcgcag cgagaggcgg tcgcggatgt cgcgcgcctt
     7801 ccggaaggcg tcgagcgccg gatcggccgt tccgctttgc tgcagcgcga gccccatgtt
     7861 ggccaggacg ttcgcgtacg acacgttcct gatgccgcgc gattcgaagt gcgcgagcgc
     7921 tttcgagtat cgcgcgatcg cctcgccgag ccggccggtg tccttgaggc agttgccgat
     7981 gttcatcagc agcatcgcat agtccgcggt gccggagagg ccgagcgcgt ccatgagggc
     8041 gagcgcggca ttgtactgcg cgagcgcgtc ggcgtggcgc tcctggcggt agaagatatt
     8101 gccgatgttc gcgacgagcg cggcgtagcc cgacgtgcgc gcgaggccga ggcggtcccg
     8161 cagggccttc gagcggttga aatactccag ggcgcgcgcg aggtcgcccc ggctgtccgc
     8221 cacggtcccc atggtgttca gcagggacgc gaaggccggc gcgttcgact cgccccggcc
     8281 ggcgagcgtc ttctcggcgc gtgtcaggta tgagatggcg cggtcgaacc gattgaggat
     8341 cacgccggcg gtgtagcccg cgcgcacgag cgcctccgcg tacgccggat cggccgcgac
     8401 ggccttttcg aagagcgcaa gcgcctcgtt cgggttcgcg tcgcggaccg cgaggccctt
     8461 cgcgtagtat tcatacgccg tgagggccgg gcgcttcttg ccggtgatgt cggcgcgcgc
     8521 cgcatcatcc agccgcggcg gctcgacgcc ggccggcgcg tttttcgtct ccgcgagcag
     8581 gcccagcacg atcttgtcct gcagatcgaa gatgccgtcg atggtcccgt cgatcttgat
     8641 ggacttctcg atcacgccgg tcgcgaccgt gacgaggcgc gcgttcacgc ggatcagcct
     8701 tccggagcgc tggtaggtcc cggtgaagat gacgtccgcc cccgcgagcg agcccacctt
     8761 cgcgatgcgc gcgtcgccga agagccccgc ctgcgcgagc tccagctcct ccaccgcctt
     8821 cttccgcccc tcctcggtga tcacgttgac gctcttcacg cgctggaggt ccgagacgac
     8881 cgtgtcggtc atcccggccg atatccacgc gagctccttg gcgccaaggt tttcgaaggg
     8941 atacacgagg atgttcatcc ggcgcgtcgc ggaatgcgaa gggatggcga tcgccgccgc
     9001 gatgaaaagg gtgatgatgg attgccgtat cggtttcatg gtttcatgta gtgagatttt
     9061 ttggaattgt ccggggccgc taccccctcc gcccttcggg cacctccccc ttcggtaggg
     9121 ggaggtgtgc aaatagtgat caacggtacc ccctttcctc aagcgaaggg agaggctggg
     9181 tgggggtagt cgtaagcatt atttcgagaa cttcttctcg cattccttct gctcggtctc
     9241 ggccttctta atggcgtcgg cttcgcattt cttcttttct gcggcgttgg tcatttttgt
     9301 gcaggggcgc tgataaaacc ccttcttgag cgcgcccatc ttccagcatt cctgcatgcc
     9361 ctgctcgcga atcttcgcct gcttctgctc gacggtttgc gcgccgacgg cggtcagggc
     9421 cccggcgatc acgacaaccg aaagagcgat agccattttt ttcatttccg accctccatg
     9481 gagattgatt gcgatgccgg cgcgcatccg acggcggccg tcatctgcgt gtagcgatgc
     9541 catacgggcg cacgatgtca attcttttgc tcgggaattg tatgcgtgga cgccagtggg
     9601 tgcccgcgga tcggccgtgg gcgatcagac gacatccagg cagatcaggc agatgtcgtc
     9661 ctcgaagctc tccgattgtc tgaatgccct cagttccgtc atgagttcgg cgagaaagcc
     9721 atcgcacggc acatgcgcat tgtcgcgcaa gatccgctcc agcgaggcgt acatgaagaa
     9781 cgcgcctccg ccgaccggcc gcgattctat gaggccgtcg gtgtagagaa gcagcttgct
     9841 tcccggttcg agcgtctcgg tgcggttttc aaagcgcttg ttggcgcgca tcagcttgtc
     9901 gttggaaaac accgccaccg cgatgcccgt acttccctgg agctggatga cgcggtccga
     9961 agacaggaga tacggtcgat tgtgccccgc gttcgaatac aggagcgagc gtgcgcgggg
    10021 atcgtagatg ccgtagaagg cggtgacgaa attacccgcg gtttggcccc gcagcacgtc
    10081 gttcatgtag ccgagcaagc ccgcgggatc ggcgagccga tcgcccgcct ggagcaggat
    10141 cgtcttgatc atggacgtga tgaacgcggc cggcacgcca tggccggaca cgtcgctgag
    10201 gaagacgccg acccgttcgg agcccgggac ggcgatgaaa tcgtagaagt cgccgcccac
    10261 cgtctccatc ggtttgtagt acgccgagat gtatggcgcc ggggtccgca gcgggatcag
    10321 gctgttctgg atttttctgg cgagggcgat ttcgttctcg atcgtccggt tgcgcatctc
    10381 gagcgaggcg ttggatttca tgaggcgctc gttgacgcgc gtgacctcct ccatcgccgc
    10441 ggccagttcc cccgtgcgct cctccacctt ccgctccagc gtggcggtgg cgtcctgttg
    10501 aatgcggatg gtctcggcct gcgccgaaat tcgttcgagc tggagcgtat tgagcttttt
    10561 gccgagcgtg aacgagaaga acaccatcat cgaaatcaag ccgatgtccg ccccccattc
    10621 cagtaggaac gcgccgggga tcacgccgat ataggtgagc ccggtcggga acagcaggac
    10681 gagcagggga aggaaggcca tcgcgaagta ttgcgcgaat tgcacccgtc gcagcagaca
    10741 gtacaggctg atgagcagca cgatgctcac gcagagaatg ccgctgaagg cggtgaagcg
    10801 cacgctgtag cgatacggga caagcagcga gagcaggatg gcggcgcaat ggatgcccgt
    10861 catcagaatg atgaaccggt ttatcgtcgg cgacaggtcc cggatgttca ggaaggaccg
    10921 gaagaacagg tacaggaaaa tcgaacagca gtggacgctg atgatgaaat tgttgttcat
    10981 ccagggatgc tgcggccaga ggtattggaa ccccgtcccg ttgagcgtcg aggagaagat
    11041 cagccccgcc gttacgaaca ggagatacca aagaaaatcc cggtcccgta ttgataaaaa
    11101 tacgaaaaag ttgtaggcga tcatgatcag caggatgccg tagagcaggc caaagaagag
    11161 cgattcattg ttgctgtttt ccgcgaggac ctgccgcgac caaatcgtga gcgggatccg
    11221 tatggccccg gtgctggtga cgcgcaggta gtatgtttgt gttccgggcc gctggagtac
    11281 gggaaaaatg aaattccgat accggtattc ccgatcataa aatggcttgg agtcgccctc
    11341 ccgcttcacg cggtagccgc cgtctcccgt cgggatgtaa agatcgacgt gatcgagatg
    11401 ggagtagccg acctccaggt accattcctt ttccttcggt tcgggatttg tcgcagagaa
    11461 cttgatccac accgcgtcgg tgatgtaacc gaacgagagc gatttgcttt ttgctgcggt
    11521 ccattgcgcg gcgcccagcc ggtccattcg gaggatttcg tcatgcgagc gtgtgccgcc
    11581 cgggtcgcgg agatattcga catgcaggcc gatcggcgtg ccgcgcaagg cgtccgcatc
    11641 aagggtgatc ggctccgcgg cgaaggcggt gatcgtagaa atgagaaaca cgaccgggag
    11701 gatgagcagg aacgaatggt tccggcgatg gcgtcccgtc gtgtgcgatt gacgtttcgt
    11761 tggcatggct tcccgcatcg atcgaagtta ctgacgggaa tgggtatatg tcaataaaaa
    11821 tagcgaggtt gcgctcgaac gtgttttcag cgtcccgttg gcgccaacga gacctacgcc
    11881 cccgcgcgga cggtcttcac gcgctcgtgc gggttgcgcg cgaagtagcc gcgcaccagc
    11941 gcgatcatcg cgtcgatcgc gctctcgcgc cggatcgtcc tgaagagctc gtgcgagaaa
    12001 tggacgttct ggcagaagta gaagcagaag cgatgccccc ggctccggtg gtacttctcc
    12061 tcgaggaagt cgcggattcc ctcgaggaca tcgacgaaca cggcctcgat gtcgatatcg
    12121 cgcacaacgt gcccgccgcc gacgagcgca tcgcagagcg cgaatatcca gggggcctgc
    12181 accgccgcgc gtccgatcat cacgccgtcg cagcccgatt cgcgcaaacg gcgcacggcc
    12241 gtttccgctg cgtcgatatc gccgttgccg atgacgggga tggagaggcg ctccttcacg
    12301 gcgcgcacgg cggaccagtc ggcggtgcgc cggaagccca gcttggcgtg ccggccgtgc
    12361 agggtgatcg cgttcacgcc ctcgtcctgg agcatgcgcg cgaagtcgag gagcgccggc
    12421 aggtccgtcc gctcgtaccc ggaacgcatc ttcacggtga ggccgcacgg cgccgcactc
    12481 cggcaggcgc gcacgatctc ccgtgcgcgc ggcgggtcgg ccagcaacgc ggcgccgccg
    12541 ccggttttca tgatgtcggg ggcggagcac cccatgttga tgtcgatcgc gcggcagccc
    12601 tgttccgcga ggcgcgccgc ggccgccgcc atcaccgccg gct
//
LOCUS       DAORHF010000096        12528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4150_length_12528_cov_6.283573, whole genome shotgun sequence.
ACCESSION   DAORHF010000096 DAORHF010000000
VERSION     DAORHF010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12528
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4150_length_12528_cov_6.283573"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1825
                     /locus_tag="PKJ16_09515"
     CDS             <1..1825
                     /locus_tag="PKJ16_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47267.1"
                     /translation="TRALDRPRTSLAPALIAAFVLLLAGSRFPLTRDQFAPTLTNVSS
                     ICPIQFTADVLRETAEVRLGRARIYRMSDPVYRNLRRAFAPIHLHTGGVPGRHLVIDG
                     NLQFYASPVPLYPAGAGILDPIPEGFVARHTMSCAPVAMIGTTREDILYFFHKRRMQY
                     AVIIDIPNPYELTFLPFRFSPAYARLIKRSLAPGGYFAQVLDVTSARAELAAAALRNF
                     TGHFAAVEGYLFGGHLVLLGSDRPGAFAVTEERVQALAAALTDRPELRGLFMSELHPL
                     AHRLYVTARPTLAGIPRLRPHHYLLRIAPVDCESPLVSGAAPVGPDAADHALREIENA
                     KLRTALAADLAKNRDIYRFIRAADCAAVAEDHAAEIAALADLKKIAAGRPDARDFIER
                     TLAAKERYYYHSGISYESRQQWQEARRRFEIALAINEGNFDANYRLGSLCITVQDFEA
                     ALKHLQQAMKLKADNPDVLYQMGVLQFFDYNPQGALEYFKKALSLGMRSVPVYINIGL
                     CYEELDQYYDARVYYQEALKIDPKDKNVLASLERLSAKMDKQKEAWGSSERRNQQEEE
                     IEEEIPLPINKSAYDKRLSDDQAKQLDPPDDEKPGAPKKPN"
     gene            1841..2689
                     /locus_tag="PKJ16_09520"
     CDS             1841..2689
                     /locus_tag="PKJ16_09520"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004885.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HNV47268.1"
                     /translation="MIEKRIIHTDAVREAVRALVRDANLVLPPPAAAALELARRGETG
                     ELARCAMDIICENAAIAERERLPLCQDCGAAIVFIELGQGVSLAGPFLLEAVNAGVAD
                     ATAAHHLRASIVSDPLRRANTGTNAPAFAHVDPVPGDSFRITVLLKGGGSENMTALRL
                     FKPTDEPATIVTWIAEAVRAAGPNPCPPLFLGVGIGGTADVAALNAKRAVLRGDAPHP
                     DPFYADLENAVRDACNATGIGPLGFGGETTVARAYIVAAPAHIATLPVAVNMNCHSLR
                     YRSMSL"
     gene            2686..3240
                     /locus_tag="PKJ16_09525"
     CDS             2686..3240
                     /locus_tag="PKJ16_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HNV47269.1"
                     /translation="MKWAPEQTPLSDARLASLAAGDALLLSGTLLTARDQAHRRIEEM
                     TRRGEALPVALSGELIYFMGPSPAPPGRAIGAAGPTTSSRMDRYTETMLSLGVRGFIG
                     KGRRSPETKELIRRRGAVYFVTFGGAAAYLGMRIIAAEPVAFADLGPEAVYRLTVRDF
                     PIIVAYDLHGGDLYEAGIRGPAGI"
     gene            3206..3808
                     /locus_tag="PKJ16_09530"
     CDS             3206..3808
                     /locus_tag="PKJ16_09530"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HNV47270.1"
                     /translation="MRLEFADRREFDLFTETLETRLTHRPPRAITRPGLRESAVLLPI
                     LYKDGQAHVLLTLRTDKVRTHKNQISLPGGGRDQGDRDVVHTALRETHEEVGIAPDDV
                     RILGIFDDFISIYGFHVATVVGIIPHPYDYAVNPDEIDACIEVPLALFRDRRYDRVQK
                     VEFEGAAYNMYHYLFGGFEIWGLTARILTDFADALLHDPA"
     gene            3883..4149
                     /locus_tag="PKJ16_09535"
     CDS             3883..4149
                     /locus_tag="PKJ16_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47271.1"
                     /translation="MRIPAAALIALALVAGCANKPVTEKEFQLVLNEYIKTEFVEGFD
                     EKPSLHQREQMLKKIVGRYSIDLERFKSFARERHAGAYKTLFEE"
     gene            4157..5527
                     /locus_tag="PKJ16_09540"
     CDS             4157..5527
                     /locus_tag="PKJ16_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HNV47272.1"
                     /translation="MMKKIRERRLHFMIFSFLLGIFAGINLTYTLVAQEPAHKYLDYF
                     HRVYQLIKTEYVDEPDTKDVFFGAIDGMVRSLKDPFSRFLDKDAFAELKEETSGNFVG
                     VGIENTIKDGQVTVIAPIADTPAMRAGVLTGDVIFKIDTTLIKNKNLNEIIKLIRGVP
                     GTRVRLFVNRKGVDEPIVFELERQPIKVASVTHAVIAGKRIGYLKIKIFSADTDREVE
                     KALRAFNQKNITRLVVDLRGNPGGYLDKAIRVCDFFLKKGDTIVTTKGRTGAGVIEEF
                     KSLESPLYTGKMILLVDQGSASASEILSGAIMDNKRGSLLGEKTFGKGSVQKIFNLNE
                     NTGIALTVAKYYTPSGASIHGTGIAPQHAVPLIIFSDADKKHLSAIYREKVVEQYVKE
                     TKCAYTEEHKKAFAEFLRRKGMPLTDTAARFLLKTEIGKLEKRPAYDLEFDTQLVRSL
                     ELLAKD"
     gene            5505..6521
                     /gene="tsaD"
                     /locus_tag="PKJ16_09545"
     CDS             5505..6521
                     /gene="tsaD"
                     /locus_tag="PKJ16_09545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HNV47273.1"
                     /translation="MNSSPKTSGAAIGLGLESSCDETAAAIVVDGRTVLSNCIYSQID
                     LHRDYAGVVPEIASRAHLEIINGLIARALAEAGVGFGDLAYVASTSRPGLVGSLLIGL
                     QSAKAIAFARGIPLVAVNHLEAHLYAPFLEGHELAHPYLGLLVSGGNTALYEVAGVGD
                     LRLLGRTTDDAAGEAFDKISKFLGLGYPGGPVIERIARTARERRRIFAKILPEPRPDD
                     YRFSYSGIKTAVINHLRQHPDEKTEEIVYAFQETALEILVRRLYAAARGRGIPRMVVA
                     GGVASNGRLRELLEEHRLPDETVVIPSPVLCTDNAAMVAGLAYHYVASGRTDSLETDV
                     HARA"
     gene            complement(6548..7063)
                     /locus_tag="PKJ16_09550"
     CDS             complement(6548..7063)
                     /locus_tag="PKJ16_09550"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HNV47274.1"
                     /translation="MEPHDSDRSMRPRGLRWCRYPFPTGRVYLFADEERLQHVHLGGA
                     LDESALGEAGTCGPLDAAIAFLDAYLLGRPAPMPPLALASLSDSVRRIYAELMHIPFG
                     GTVTYGELALRAGIARGAARFVGTAMARNPLPIFIPCHRVIRADGSLGGFSAGVAVKR
                     FLLQHERALAG"
     gene            7230..8564
                     /locus_tag="PKJ16_09555"
     CDS             7230..8564
                     /locus_tag="PKJ16_09555"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HNV47275.1"
                     /translation="MIGCSYGRFFQVTVAGGSYQEGLTTHIQGVPAGILLDESEIYAE
                     LLQRKPGQGELSSPRREPDIPVIYSGVNAADTMPGFSNEGYTNGTPLVVLIPNLDRHF
                     EHIDQYQRTNRTPRPGHASYATYQKYGSWDDSIGAGIFSGRYTSTIVAAGAIAKRILA
                     DHGIDVFAYVKEAAGIAMPAMEYPVIRRRVGDFKAARRDMDPVFNLVYREGRIKPGMR
                     FLEKMAALAEIERMVPDITRPPLSDAEVAALAERGLHPVLNCPDLEAARAMYDRIIAI
                     RDGGDSSGGLVEVVATGVPAGLGEPVFDKLDGELGRMMSIGTVKAVEIGAGMRVKDMT
                     GSACNDQMHMQNGAIAFSGNNSGGITGGVTTGQDIVVRLAVKPTPTIAKPQKTVDKVS
                     LEDAELAAVTRRDPTIVSRVWPVAEAFTAIILLDQYILNLATQGMRAPYRDK"
     gene            8571..9794
                     /locus_tag="PKJ16_09560"
     CDS             8571..9794
                     /locus_tag="PKJ16_09560"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HNV47276.1"
                     /translation="MTPRRRHGTLRLTRCLAAGLIVFICLPRTPLSPSRADSVSLPIV
                     RIDAREYVSLHDLVAALRIDTSFDIITQRGKLYRDSRVAVYQVGFAVALANGALVRAA
                     YPVTRKNGEVLIPEALFRGMFSRLYPDRDITRAESAFTVTRPRDGTPRDRTKPDDRDR
                     VDPADRRKDRIGFIVVDAGHGGHDPGAIGRGRIKEKTITLKVARALERHLRAQLPGVT
                     VIMTRTGDSFIELGRRTEIANRQLKSNTNGIFVSIHVNASIARRIAGFETYFLSQNPT
                     NEEARTTAALENNVVILEDRSRRKSYNDVEYVEALMLTTQIQKESALLSKTILREMKA
                     TMRPARPRGVKKADFFVLRGALMPATLVEIGYITNAGEAQQLTRPEYQRRVAAGIGNG
                     VIEFIKKYNTMIKIK"
     gene            9849..10514
                     /locus_tag="PKJ16_09565"
     CDS             9849..10514
                     /locus_tag="PKJ16_09565"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNV47277.1"
                     /translation="MNIAHIKQAVASAKDRIVVGAREIRPRERIARFLPAVQELIEKG
                     RDRIPTDATRRNRLYVLVIALICLFDYLAISYLTDRNPLAIYPSLPVLDSRATVTVYL
                     PNPDGKTLIKETRRVEPLDDREEFARTLVKLVARGSDFENTSFTVPVDVIVRKVWIHE
                     ERCAVDIALAQVKESMKVLPGSEVMFRQAVAKTITENIPSVKIVHVLEKGIPNRNIWE
                     MGI"
     gene            complement(10557..11234)
                     /locus_tag="PKJ16_09570"
     CDS             complement(10557..11234)
                     /locus_tag="PKJ16_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47278.1"
                     /translation="MRIEILSCQLQKLALSAGQSLLSILALMFSKGKFMKIKVQSVIL
                     IGALVMVSAASVVLENEINAQGTTHKIGQKGPAGGWIFYDKGNSDGGWRYLEAAAEDQ
                     GLAKWGCSEKTIPGAQGKAIGTGKANTDAIIKHCEEAEMAAKVAVNYRGGGKSDWFLP
                     SIDELALMYKNLHKARVGGFRIYDVYWSSSEAISSNTAWVRFFDKDVQDYSDKQYRKK
                     VRAVRAF"
     gene            complement(11261..12097)
                     /gene="dapF"
                     /locus_tag="PKJ16_09575"
     CDS             complement(11261..12097)
                     /gene="dapF"
                     /locus_tag="PKJ16_09575"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HNV47279.1"
                     /translation="MPFTKMHGIGNDYLYLDARTRAVKNPGRLSREMSDRHFGVGSDG
                     LILVMKSKRADFRMRIFNPDGSEAEMCGNGIRCFAKYVYDRRFTKKTVMAIETMAGIM
                     PVRVSVRGGKVSAATVDMGEPVLQRERIPMIGAPGMVIDEPLPLDGVRFNVTSLSMGN
                     PHAVIYVEDVERFPVEKYGPLIERHDLFPNRTNVEFVEVVNDREIIQRTWERGTGETL
                     ACGTGASAAVVAGCLSRNAARSVTVRLRGGALRVEWKERDNRVYLTGPAVEVFEGSWP
                     NA"
     gene            12201..>12528
                     /locus_tag="PKJ16_09580"
     CDS             12201..>12528
                     /locus_tag="PKJ16_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47280.1"
                     /translation="MKHTALILALPAGAALAIAIGLFALPTGALDDYLVTTLPGWERR
                     RTEITSRLLEVPIVLYHNIDGKGPFSLGLDTLRAHFEYYRTAGIRVIPLAELVDRLER
                     PRPYRER"
BASE COUNT         2330 a         4316 c         3778 g         2104 t
ORIGIN      
        1 cacgcgcgcg ctcgaccgac cgcggacctc gctcgccccg gcgctcatcg ccgccttcgt
       61 cctcctgttg gccggctccc gcttcccgct cacccgggac cagttcgccc ccacgctcac
      121 caacgtatcg tcaatttgcc ccatccagtt caccgccgat gttcttcgcg aaacggccga
      181 ggtccgtctg ggacgcgcgc ggatataccg catgtccgat cccgtgtacc gcaatctgcg
      241 ccgggccttc gcccccattc acctgcacac gggcggcgtc ccgggccgcc acctggtcat
      301 cgacgggaac ctgcagtttt acgcatcgcc ggtcccgctt tatccggcag gggccggcat
      361 cctcgatccc atccccgagg gcttcgtggc gcgccacacg atgtcgtgcg ccccggtcgc
      421 gatgatcggc accacgcgcg aggacatcct gtacttcttc cacaagcggc gcatgcagta
      481 cgccgtgatc atcgacatcc ccaatcccta tgagctcacc ttcctgccgt ttcgcttctc
      541 gccggcctat gcgcgcctga taaaacgctc ccttgcgccc ggcggctatt tcgcgcaggt
      601 gctggacgtc acgagcgcgc gcgccgagct ggcggcggcc gccctgcgaa acttcaccgg
      661 gcacttcgcc gccgtcgagg ggtatctctt cggcggccac ctggtcctgc tcggatcgga
      721 ccgtcccggt gccttcgccg tgaccgagga gcgcgtgcag gcgctcgccg cggcgctcac
      781 ggaccggccg gagctgcgcg gcctgttcat gagcgagctg catccgctcg cgcaccggct
      841 ctacgtcacg gcccgcccga cgctcgcggg cattccccgc ctgcgtccgc accactacct
      901 gttgcgcatc gccccggtcg attgcgagag cccgctcgtg tccggcgccg cgccggtcgg
      961 ccccgacgcg gcggatcatg cgttgcgcga aattgagaat gcgaagctcc gtacggcgct
     1021 cgcggccgac ctcgcgaaga accgagacat ctaccggttc atccgggcgg ccgattgcgc
     1081 ggccgtcgcc gaggaccatg cggcggagat cgccgcgctg gccgacctca agaaaatcgc
     1141 cgccggccga ccggacgccc gggacttcat cgagcgcacg cttgccgcca aggagcgcta
     1201 ttactaccat tccggcattt cctacgaatc gcgccagcag tggcaggagg cgcgccgccg
     1261 cttcgagatc gccctggcca tcaacgaggg aaatttcgac gcgaactacc ggctgggctc
     1321 gctgtgcatc accgtgcagg acttcgaggc ggcgctcaag cacctgcagc aggcgatgaa
     1381 gctcaaggcc gacaatcccg acgtgctcta ccagatgggc gtgctgcagt ttttcgatta
     1441 caacccgcag ggcgcgctcg agtatttcaa gaaggcgctc tccctgggga tgcgctccgt
     1501 cccggtgtac atcaacatcg gcctctgcta cgaggagctc gaccagtact acgacgcgcg
     1561 cgtctactac caggaggcgc tcaagatcga cccgaaggac aaaaacgtcc ttgcgagcct
     1621 ggagcgcctc tcggcaaaaa tggacaagca gaaggaggcg tggggctcaa gcgagcggcg
     1681 caaccagcag gaggaggaga tcgaggagga gatcccgctg ccgatcaaca agtccgccta
     1741 cgacaagcgc ctcagcgacg accaggccaa gcagcttgat ccgcccgacg atgagaagcc
     1801 gggcgctccg aaaaaaccca actgaccggc ggatgcgcgc atgatcgaaa aacgcatcat
     1861 acacaccgac gcggtccgcg aggccgtgcg cgcgctcgtg cgggacgcga acctcgtcct
     1921 gccgcccccg gcggccgcgg cattggagct cgcgcgccgg ggcgagacag gcgagctggc
     1981 gcgctgcgcc atggacatca tctgcgaaaa cgcggctatc gccgagcgcg agcggcttcc
     2041 gctctgccag gattgcggcg cggcgatcgt cttcattgaa ctggggcagg gggtgtcgct
     2101 tgcggggccg ttcctcctcg aggccgtcaa cgccggcgtc gccgatgcga ccgcagcgca
     2161 ccacctgcgc gcctccatcg tgtccgaccc gctccggcgc gcaaacaccg gcaccaatgc
     2221 gccggccttc gcgcacgtgg acccggtccc gggcgattcg tttcgcataa cggtactcct
     2281 caagggcggc ggctcggaaa acatgaccgc cctccggctc ttcaagccca ccgacgagcc
     2341 cgcgacgatc gtgacgtgga tcgccgaggc cgtgcgcgcg gcggggccca atccctgccc
     2401 gccgctcttc ctgggcgtgg gcatcggcgg cacggccgac gtcgcggcgc tcaacgccaa
     2461 gcgcgcggtc ctgcgcggcg acgcgcccca tcccgatccc ttctatgcgg acctcgagaa
     2521 cgcggtccgc gacgcctgca atgcgaccgg gatcggcccg ctgggctttg gcggcgagac
     2581 caccgtggcg cgcgcataca tcgtcgcagc cccggcccac atcgcgacgc tgccggtggc
     2641 cgtgaacatg aactgccatt cgctgcgcta ccggagcatg tcgctgtgaa atgggcgccg
     2701 gaacagaccc cgctctccga cgcgcgcctc gcctcgctcg cggccggcga cgcccttctc
     2761 ctctcgggaa cgctcctcac ggcgcgcgac caggcgcatc ggagaatcga agagatgacg
     2821 cggcgcggcg aggcgctccc cgtcgcgctc tccggcgaac tgatctactt catgggcccg
     2881 agcccggcgc cccccggccg cgcgatcggc gccgcgggac cgaccacctc ctcgcgcatg
     2941 gaccgctaca ccgagacgat gctctcgctg ggcgtgcgcg gcttcatcgg caaggggcgt
     3001 cgctcgccgg aaaccaagga gctcatccgt cggcgcgggg ccgtctattt cgtgaccttc
     3061 ggcggcgccg cagcctatct gggcatgcgc atcatcgccg ccgaaccggt cgcattcgcc
     3121 gacctgggac cggaggcggt ataccgattg acagtgcgcg actttccaat catcgtggcc
     3181 tacgaccttc acggcggtga cctgtatgag gctggaattc gcggaccggc gggaatttga
     3241 cctatttacc gaaacgctcg aaacgcgcct gacgcaccgc ccgccgcgcg ccatcacccg
     3301 gcccggactt cgcgaatccg ccgtactcct cccgatcctc tacaaagacg ggcaggccca
     3361 tgtgctgctc acccttcgga ccgacaaggt gcgcacgcac aagaaccaga tctcgctccc
     3421 cggcggcggc cgcgatcagg gcgaccgcga cgtcgtgcac acggcgctcc gcgaaaccca
     3481 cgaggaggtg gggatcgcgc cggacgacgt gcgcatcctc ggaatcttcg acgatttcat
     3541 ctccatctac ggcttccatg tcgccaccgt cgtcggcatc atcccccatc cctacgacta
     3601 tgccgtcaat cccgacgaga tcgacgcctg tatcgaggtc cccctcgcgc tctttcgcga
     3661 tcgacgctac gaccgggtgc agaaggttga gttcgagggc gccgcctaca acatgtacca
     3721 ttacctcttt ggaggattcg agatctgggg gctcaccgcg cgcatcctca ccgacttcgc
     3781 ggatgcgctc ctgcacgacc ctgcgtaatc cgcgaaaacg tattgacaac accccgcgcc
     3841 ccgccgtagc gttcacggac cacacaggaa cggagatatc ccatgcgcat tccggccgcc
     3901 gcactcatcg cactcgcgct tgtcgcgggc tgcgcgaaca aacccgtcac ggagaaggaa
     3961 ttccagctcg tgctgaacga atatatcaag accgagttcg tcgagggctt cgacgagaaa
     4021 ccgtcgctcc atcagcgcga gcagatgctc aagaagatcg tgggccgcta ctcgatcgac
     4081 ctcgagcgct tcaaatcatt cgcacgcgag cggcacgccg gcgcgtacaa gacgctcttc
     4141 gaggaatgat cacgacatga tgaaaaaaat ccgggagcgc cggctccact tcatgatctt
     4201 ctcgttcctg ctggggatct tcgcggggat caatctcacc tacaccctgg tcgcgcagga
     4261 gcccgcgcac aagtacctgg actacttcca ccgcgtctat cagctcatca agaccgagta
     4321 tgtcgatgag cccgacacca aggacgtctt cttcggggcg atcgacggca tggtccgctc
     4381 cctcaaggac cccttctcgc gctttctgga caaggacgcc ttcgccgagc tcaaggaaga
     4441 gacctccggc aacttcgtgg gcgtagggat cgagaacacc atcaaggacg gccaggtgac
     4501 ggtgatcgcg ccgatcgccg acacccccgc gatgcgcgcg ggcgttctca ccggcgacgt
     4561 gatattcaag atcgacacca ccctcatcaa gaacaagaac ctcaacgaga tcatcaagct
     4621 gatccgcggc gtcccgggca cgcgcgtccg gctcttcgtg aaccgcaagg gcgtcgatga
     4681 gcccatcgta ttcgaactcg agcggcagcc catcaaggtc gccagcgtta cgcatgcggt
     4741 gatcgccggt aagcggatcg ggtaccttaa aatcaagatc ttcagcgcgg acaccgaccg
     4801 cgaggtcgaa aaggcgctgc gcgccttcaa ccagaaaaat atcacccgcc tcgtcgtgga
     4861 cctgcgcggc aatcccggcg gttacctcga caaggccatc cgcgtgtgcg atttcttcct
     4921 gaaaaagggc gataccatcg ttaccacgaa ggggcgcacg ggggcgggcg tgatcgagga
     4981 gttcaagtcc ctcgaatcgc cgttgtacac cgggaagatg atcctgctgg tggaccaggg
     5041 gagcgcgtcg gcctcggaga tcctctcggg cgcgatcatg gacaacaagc gcggctcgct
     5101 cctcggcgag aagaccttcg gcaagggctc cgtgcaaaag atcttcaacc tcaacgagaa
     5161 cacggggatc gccctcacgg tcgcgaagta ctacacgccg tccggggcat ccatccacgg
     5221 aacgggcatc gcgccgcagc acgcggtgcc gcttatcatc ttttccgacg ccgacaaaaa
     5281 gcacctgagc gccatctacc gcgaaaaggt cgtggagcag tacgtcaagg agacgaagtg
     5341 cgcctacacc gaagagcaca aaaaggcctt cgccgaattt ctgaggcgca agggcatgcc
     5401 cttgacggac acggccgcgc gcttcctgct caagaccgag atcgggaagc tcgagaagcg
     5461 ccccgcctat gacctcgaat tcgacacgca gctcgtccgc tcccttgaac tcctcgccaa
     5521 agactagcgg cgcggcgatt ggcctcgggc ttgaaagctc ctgcgacgag acggccgcgg
     5581 cgatcgtcgt cgacggccgg acggtccttt cgaactgcat ctacagccag atagacctcc
     5641 accgggacta tgcgggcgtg gtcccggaga tcgcctcgcg cgcacacctg gagatcatca
     5701 acgggctcat cgcgcgcgcg ctcgcggagg ccggcgtggg gtttggcgac cttgcgtacg
     5761 tcgcttcgac cagccggccc gggctggtgg gttcgctcct catcggcttg cagtcggcca
     5821 aggcgatcgc ctttgcgcgg ggcattccgc ttgtggccgt gaaccacctg gaggcgcacc
     5881 tctacgcgcc ctttctcgag ggacacgagc tcgcgcatcc ctatctgggc ctgctggtct
     5941 ccggcgggaa caccgcgctc tacgaggtcg cgggcgtggg ggaccttcgg ctccttggcc
     6001 gcaccacgga cgacgcggcg ggcgaggcct tcgacaagat atcgaaattc ctggggctgg
     6061 gctatcccgg cggtcccgtc atcgagcgga tcgcccggac cgcgcgcgaa cggcggcgca
     6121 tcttcgcgaa gatactcccc gaaccgcgac cggacgacta ccgtttctcc tacagcggga
     6181 tcaaaaccgc cgtcatcaac cacctgcggc agcacccgga cgaaaaaacc gaggagatcg
     6241 tgtacgcctt tcaggaaacg gcgctggaga tcctcgtccg gcgcctctac gccgccgcgc
     6301 gcggacgcgg gatcccccgc atggtggtcg ccggcggtgt tgcgtccaac ggcaggttgc
     6361 gcgaactcct cgaggagcac cggctcccgg acgagacggt cgtcatcccc tcgcctgtcc
     6421 tgtgcacgga caacgcggcg atggtcgcgg ggctcgcgta ccactacgtc gcgtcgggac
     6481 ggaccgacag cctcgagacc gacgtgcacg cccgcgcctg atccgcgcgg gacgacgcca
     6541 acccggacta tccggcgagc gcgcgctcgt gctgcagcaa aaatcgcttc acggcaacgc
     6601 ccgcggaaaa tccgccgagc gatccgtcgg cgcggatgac gcggtggcag ggtatgaaga
     6661 tcggcagcgg attgcgcgcc atcgccgttc caacgaaacg cgccgcgccg cgcgcaatcc
     6721 ccgcgcgaag cgcaagctcg ccgtaggtta cggtgccccc gaagggaata tgcatgagct
     6781 ccgcatagat gcgccggact gaatccgaga gggaagcgag cgcgaggggc ggcatcgggg
     6841 cgggtcgccc aagcagatag gcgtcgagaa aagcgatcgc ggcatcgagc gggccgcacg
     6901 tgcccgcctc ccccagcgcg ctctcgtcca gcgcgccccc gagatggaca tgctgcaggc
     6961 gctcctcgtc ggcgaacagg tacacgcgcc cggtcgggaa gggatagcgg caccagcgca
     7021 atccgcgcgg gcgcatcgat cggtcactgt cgtgcggttc catgatcccg ccggactatc
     7081 agcgggcgag gattttgtaa aaacaaatta tcatcatccc ccttttttac ttgaatttta
     7141 gcgccgccgc gcccaggttt cgctctgcga ttggtccggc ctgcttcgcg ggttcgaacg
     7201 ccaatcagtc tgtcctcaag gggtgtgtga tgatcgggtg cagttacgga cgtttttttc
     7261 aggtcacggt cgcgggcggt tcctatcagg aggggctgac cacgcacatc cagggcgtcc
     7321 ccgcggggat cctgctcgac gagagcgaga tctacgcgga gctcctccag cgcaaacccg
     7381 gccagggaga gctttcgtcg ccccgtcgcg agcccgacat ccccgtcatc tacagcgggg
     7441 taaacgccgc cgacacgatg ccgggctttt ccaacgaggg atacacgaac ggaacgccgc
     7501 tcgtggtgct catcccaaac ctcgaccggc atttcgagca catcgaccaa taccagcgca
     7561 ccaaccgcac gccgcgcccc ggccacgcct cctacgcgac gtaccagaag tacggatcgt
     7621 gggacgattc catcggcgcg gggatcttca gcgggcgcta tacgtcgacc atcgtcgccg
     7681 cgggcgcgat cgccaagcgc atcctcgccg atcacggtat cgacgtgttc gcctatgtca
     7741 aggaggcggc ggggatcgcc atgccggcga tggagtatcc ggtcatccgc cgacgcgtgg
     7801 gcgatttcaa agcggcgcgg cgcgacatgg accccgtgtt caatctggtg tatcgcgagg
     7861 gccgcatcaa gccgggcatg cgctttctcg agaagatggc ggcgctggcc gagatcgagc
     7921 ggatggtccc ggacatcacg aggccgccgc tctccgacgc ggaggtcgcc gcgctcgccg
     7981 agcgggggct gcaccccgtg ctcaattgtc cggacctcga ggccgcgcgc gccatgtacg
     8041 accgcatcat cgccatccgc gacgggggcg attccagcgg cggcctggtt gaggtggttg
     8101 ccaccggcgt cccggcggga ctgggcgagc cggtgttcga caagctcgac ggcgagctgg
     8161 gacggatgat gtcgatcggc accgtgaagg ccgtggagat cggcgccggc atgcgcgtga
     8221 aggacatgac cgggagcgcc tgcaacgacc agatgcacat gcaaaacggc gcgatcgcct
     8281 tctccggcaa caacagcggc ggcatcaccg gcggcgtcac caccggccag gacatcgtgg
     8341 tgcgcctcgc cgtgaagccc acgccgacca tcgccaagcc gcagaagacc gtcgacaagg
     8401 tatcgctcga ggacgccgag ctcgcggcgg tcacgcgccg cgaccccacc atcgtttcgc
     8461 gcgtgtggcc cgtcgccgag gcattcacgg ccattatcct gctcgaccag tacatcctga
     8521 atctggccac ccaggggatg cgcgcgccgt accgggacaa gtaggcgggc gtgacaccga
     8581 ggcgccgaca cgggacgctc cgactgacgc ggtgtctcgc ggccggcctc atcgtgttca
     8641 tctgcctgcc gcgaacgccc ctgagcccgt cgcgtgcgga ttccgtttcg ctgcccatcg
     8701 tgcgcatcga cgcgcgcgag tacgtttcgc tgcacgacct ggtcgccgcc ctgcggatcg
     8761 acacctcctt cgacatcatc acccagcgcg gcaagctgta ccgcgacagc cgcgtcgccg
     8821 tataccaggt ggggtttgcg gtggccctgg cgaacggggc cctggtcagg gcggcctatc
     8881 cggtgacgcg gaaaaacggc gaggtgctga tccccgaggc gctcttccgg ggcatgtttt
     8941 cccgattgta tccggaccgc gacatcacgc gcgccgagtc ggctttcacg gtgacccgcc
     9001 cccgtgacgg gacaccgcgc gaccggacga aacccgacga tcgcgaccgc gtcgatcccg
     9061 ccgaccggcg caaggaccgc atcggcttca tcgtcgtcga cgccgggcac ggcggccacg
     9121 atccgggcgc catcggccgc gggcgcatca aggagaaaac gatcacgctc aaggtcgcgc
     9181 gggcgctcga gcgtcatctg cgcgcgcagc ttcccggcgt cacggtgatc atgacccgaa
     9241 cgggcgacag cttcatcgag ctggggcgcc gcacggagat cgccaaccgg cagctcaaaa
     9301 gcaacacgaa cggcatcttc gtgagcatcc acgtgaacgc ctccatcgca cgccgcatcg
     9361 ccggcttcga gacctacttt ctgtcgcaaa acccgaccaa cgaggaggcg cgcaccaccg
     9421 ccgcgctcga gaacaacgtg gtcatcctcg aggaccgctc gcggcggaaa tcgtacaacg
     9481 acgtggagta tgtcgaagcg ctcatgctca ccacgcaaat ccagaaagaa agcgcgctct
     9541 tgtccaagac cattctgcgc gagatgaaag cgaccatgcg tccggcccgt ccgcggggcg
     9601 tcaaaaaggc cgattttttc gtgctccggg gggcgctcat gcccgcgacc ctcgtcgaga
     9661 tcggctacat caccaatgcg ggggaggcgc aacagcttac gcgcccggag tatcagcgtc
     9721 gggtcgcggc ggggatcgga aacggcgtga tagagtttat taaaaaatac aatacgatga
     9781 taaaaattaa atgacttttt ggcctccggc gccgtcaata gccgcatcgg acggggggac
     9841 gcacaccaat gaacatcgca cacatcaaac aggcagtcgc ttcggcgaag gaccgcatcg
     9901 tcgtcggcgc tcgcgagatc cggccgcgcg agcggatcgc ccgattcctt cccgcggtgc
     9961 aggagctcat cgagaagggg cgcgaccgca tcccgaccga cgccacccgg cggaaccgcc
    10021 tgtacgtgct cgtcatcgcg ctcatctgcc tgttcgatta tctcgccata agctatctta
    10081 cggaccgcaa tccgctcgcg atctatccgt cgctgcccgt gctcgattcg cgcgcgaccg
    10141 tcaccgtcta tctgcccaac cccgacggca aaacgctcat caaggagacg cgacgcgttg
    10201 agccgctcga cgatcgtgag gagtttgcgc gcaccctggt gaagctagtc gcccgcggct
    10261 ccgatttcga gaacacctcg ttcacggtcc cggtcgacgt catcgtgcgg aaggtgtgga
    10321 tccacgagga gcggtgcgcc gtggatatcg cgctcgcgca ggtgaaggaa tcgatgaagg
    10381 tgctgccggg ctccgaggtc atgttccgcc aggccgtcgc gaaaacgatc acggaaaaca
    10441 tcccgtcagt gaaaatcgtc catgtcctcg aaaagggcat ccccaaccgg aacatctggg
    10501 agatgggaat ttaacaaacg attgttccgt cacgcgcatc acaatcccac aacagcttaa
    10561 aatgcccgga ccgcccgaac cttttttcgg tattgcttat cgctatagtc ctgtacatct
    10621 ttatcgaaga accgtaccca cgcagtgtta ctcgaaatgg cctcagatga gctccaataa
    10681 acatcataaa tacggaaacc gccgacacgg gctttatgta aatttttata catcagagcg
    10741 agctcgtcga tcgatggtaa aaaccagtcg ctttttccgc cgccccgata gttaaccgca
    10801 acttttgcag ccatttctgc ttcttcacag tgtttaatta ttgcatcagt atttgctttt
    10861 ccggttccaa tggctttgcc ctgtgctccg ggaattgttt tttcggaaca accccatttt
    10921 gcgagtccct gatcctccgc tgcggcttca agatatctcc accctccatc agaattcccc
    10981 ttgtcataga aaatccatcc ccccgccggg cctttctgtc caatcttatg cgtcgttccc
    11041 tgtgcgttaa tttcattttc cagtacaaca ctcgccgccg agaccatgac cagtgctcca
    11101 attaatatta ctgattgtac cttgatcttc atgaatttcc ccttggaaaa cattaatgca
    11161 agaatggaga gcagagattg ccccgcactg agagccaatt tctgcaactg gcaggataag
    11221 atctctattc tcatcgcatc tcctgcgcgg agtaacgacg ttacgcgttc ggccagcttc
    11281 cctcgaacac ctcgaccgcc ggaccggtaa gatacacgcg attgtcgcgc tccttccatt
    11341 ccacgcgaag ggcgcccccc cgcaggcgga ccgtcaccga acgcgcggcg ttgcgcgaca
    11401 ggcatcccgc caccaccgcg gcggaggccc cggtgccgca ggcgagcgtc tcgccggtgc
    11461 cgcgctccca ggtgcgctgg atgatctcgc ggtcgttcac gacctcgacg aactccacat
    11521 tggtgcggtt ggggaagagg tcatggcgct cgatgagcgg accgtatttc tcgaccggga
    11581 agcgctcaac gtcctccacg taaatgaccg cgtgcgggtt ccccatcgaa agcgacgtga
    11641 cgttgaagcg gaccccgtcg agcggcagcg gctcgtcgat caccatgccg ggcgcgccga
    11701 tcatggggat gcgctcgcgc tggagcaccg gctcccccat gtcgacggtg gccgcgctca
    11761 ccttcccgcc gcgaacggac acccgcaccg gcatgatgcc ggccatggtc tcgatggcca
    11821 tcacggtttt tttcgtgaat cgccgatcgt acacgtactt ggcgaagcac cggatgccgt
    11881 tgccgcacat ctcggcctcg ctcccgtcgg gattgaagat ccgcatgcgg aaatcggcgc
    11941 gcttcgactt catgacgagg atcagcccgt ccgaaccgac cccgaagtgc cggtcgctca
    12001 tctcgcgcga gagccgtccg gggtttttca ccgcgcgggt gcgcgcgtcc aggtacagat
    12061 aatcgtttcc gatgccgtgc atcttcgtga agggaatcat gccgccgcct ccgttgccgc
    12121 acggatcatg ccccgacggc gcataaaaat caacataaaa aatacacttt ttccgccgcc
    12181 ggcggaacga tatgtatagt atgaaacaca cggccctcat cctcgccctg ccggcgggag
    12241 cggcgctcgc gatcgccatc ggcctgttcg cgctcccgac gggcgccctc gacgactacc
    12301 tcgtgaccac cctgcccggc tgggagcgcc gccgcacgga gatcacctcg cgcctgctcg
    12361 aggtccccat cgtgctgtac cacaacatcg acggcaaagg gccattctcg ctcgggctcg
    12421 atacgctgcg cgcgcacttc gagtactatc gcacggccgg catacgggtg atcccgctcg
    12481 ccgagctggt cgaccgcctc gagcggccgc ggccctatcg cgagcggg
//
LOCUS       DAORHF010000097        12324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4249_length_12324_cov_5.949874, whole genome shotgun sequence.
ACCESSION   DAORHF010000097 DAORHF010000000
VERSION     DAORHF010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12324
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4249_length_12324_cov_5.949874"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="PKJ16_09585"
     CDS             <1..373
                     /locus_tag="PKJ16_09585"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HNV47281.1"
                     /translation="HVDFYKITAGEKIHTHVPVELTGSAEGERMGGVVEFLERELEIE
                     CLPDEMPEKIVIDITTLGIGKSIQQKDVPTSGSLKILGDAHRVIVACVVPQKASEEAA
                     EPAAEVAETKAAEPEKEKKSE"
     gene            480..1094
                     /gene="pth"
                     /locus_tag="PKJ16_09590"
     CDS             480..1094
                     /gene="pth"
                     /locus_tag="PKJ16_09590"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV47282.1"
                     /translation="MKLIVGFGNPGEKFVNNRQNIGFKVVEILANNENIDVRVKKKKS
                     VIGRGSVNGTEVVLLKPQTFVNLIGESVLYIASFLRINVREIICVLEDPTLPVGELRV
                     DYRLSKIKHEGIESITKALKSDKFAKVRVGVGVPPKSMTVEQYLLTDFTDRENLILID
                     VLNKAEEAVKALITNTIESVQENYNPEGPVRIERRRVRKVYVKR"
     gene            1916..3412
                     /locus_tag="PKJ16_09595"
     CDS             1916..3412
                     /locus_tag="PKJ16_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47283.1"
                     /translation="MKKPLFDQSGEWEELLKNDLGQSPKPNPETTGSKTVGASASAGL
                     NFDIKPEELESYLNRYVMGQESAIEVIATKVCTHFNRMKLEATIPEDERIVGNIKSNM
                     LLIGPTGVGKTYIIKLIARRIGVPFVKADATKFSETGYVGGDVEDLVRELVHEAEGDI
                     KKAEYGIIYLDEIDKIASAGTGHGPDVSRTGVQRNLLKLMEEAEVDLKTPHDLASQVE
                     AAMEAQRTGKVTRKKINTRNILFIVSGAFSSIEDIIRRRLNMQAIGFDKAYASKTDGP
                     ALLTLIKTEDLMQFGFESEFIGRLPVTVVLNELDEEGLYRILKNRYSTVVLGKKFDFR
                     SYGIELDFTDDALRTLARRAHAEKTGARGLLTVFEKVLIKFEKTLPSTEIRQLTVDCD
                     VVEQPEAALRKMLLSDSIKSFQKDFLLKNGIYLEFDREAAEALHRMAGERNCSIKQLC
                     EELFHDYSYGIKLMEQDTFRITRDAVERPDAYLEEFIRKNYPKGRGAS"
     gene            3498..3887
                     /locus_tag="PKJ16_09600"
     CDS             3498..3887
                     /locus_tag="PKJ16_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HNV47284.1"
                     /translation="MEQLFAQDMSGGGGGGSIVTTIIYLIFLVAWIAGMWKVFEKAGK
                     PGWAAIVPIYNIIVLLQIINKPLWWIVLMLIPIVNFIIFLIVSMELAVCFGKSKGWGV
                     GLLFFFGFVGYPLLGFGDATYTAPAGS"
     gene            4077..4787
                     /locus_tag="PKJ16_09605"
     CDS             4077..4787
                     /locus_tag="PKJ16_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47285.1"
                     /translation="MDFDLKKFIRDTRAVLLTPRAYFSTMDTTGGYWEPVIRAGIYGI
                     VAGVLQLAWRLLFPQPVPALAGVAGSTGVSIAPFITPIIAVLIGLFASAILLLIVSAI
                     CGGSTNVEAAIRCGAALTAVAPVNALFGFVAAIHPAARAAVSFVITLYAIALMYHALV
                     RALSCAEKPARIAAGIIAAIPAVAFAIALTSALKPKDAIQQLSGDRQQREELERTERE
                     ALEFIDRLKEMQRRQKGE"
     gene            complement(4741..5538)
                     /locus_tag="PKJ16_09610"
     CDS             complement(4741..5538)
                     /locus_tag="PKJ16_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47286.1"
                     /translation="MTAERVEFRNGRNQRIAGRLHRADPPASAGAIFCHGLFSTKDGY
                     KITRLADDIRAAGLTLLAFDFSFVGESEGRIEDLSIEQEVDDLACAVRFMRGQGFTRV
                     HLIGSSMGGTVSVLYAARHADSVDSLVLIATPVDLPGLLYRMTGMRDTDRLPEEGATV
                     VQGIAINNRFFREIGALDPRGALPGISAPVLVIHGECDDVVDPENARRIVSGVRGEAR
                     LVNIPDGDHNLVRDEDLVVIRANLIEWLRDRAFTPPSGAAASPSVDQ"
     gene            complement(5535..6017)
                     /locus_tag="PKJ16_09615"
     CDS             complement(5535..6017)
                     /locus_tag="PKJ16_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47287.1"
                     /translation="MGNNRSALWASVSAAVVAIALCATSVSAAGRPLGFAEIAGRWRL
                     MYSGRYGFEFTFTKNYRAICVMYLRSHAYVFRGVYTIEEGDRIRFNVSDMKNEKSAGH
                     INLKSGFVKASSSYFIFHAGLDRSRGRPTLVLKPERIIIDGRNSDGYFEPEMRLEKSG
                     "
     gene            complement(6007..7911)
                     /locus_tag="PKJ16_09620"
     CDS             complement(6007..7911)
                     /locus_tag="PKJ16_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV47288.1"
                     /translation="MAHDETDARMTRRDFLGRALAGTAAIALSGMPGCGARTPVERLR
                     VNYEKPLRLVNCSVIDVKTGTVRRGAVVTIMRDTIASITTKDATAHPGETFDLGGKYV
                     IPGLIDAHCHITMPGTLLFDFFELYDDLKQNRRNYTECIRAGITAVRDTGALVSELHR
                     AIDDIAGGGLIGPRVFYCNSIINIAGSHPDIRPSDVSIFGGPTSLITGSVTVDFTDLS
                     DLKYKLEQNVERGASFIKLTVDNRSVFCGRGEIPVYTDEQLRHIFAFAERKGLPVIAH
                     NHFKYGFDRITRYPLHSLEHSLSDAALSDDEAAMMASKKIACVPTMVIASYLSPGEAF
                     EHQPATLKTETARNEIAINRAYYDSLASRYVQSRIHRNNRANMELFRVRDCGRYRAEK
                     KFLPNPELYYGVLRHGPENLRRMFRAGVLIGCGTDAGMPFSYHGALWRELELMHRVGI
                     SAKDVLRCATYNNALILGAADKMGSVEPGKWADLAVLDANPLENIQAYRSPALVFKGG
                     RLMYSAYELDRREGGIVIAPVYEGDTRDGRREGAGRYRWRDGSTFEGSWRDDRRDGPG
                     EYRYPGGEAYLGEFKADRRNGVGEYTWPTGERYQGQWKDGRMHGYGTFTTRDGTRFTG
                     RFEEGKYRGK"
     gene            complement(7969..8451)
                     /locus_tag="PKJ16_09625"
     CDS             complement(7969..8451)
                     /locus_tag="PKJ16_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47289.1"
                     /translation="MAIDTTLYVHETTIELIDSACAIMGCSRSRAIVMLFRRAMADRR
                     KRPNTGSAIRYQRRDALRRWRTVHVRFREDEADYVKDMRNFFKLSDSLILAQAARQYL
                     DDVVKQSCRDGKNDNYRFPNYILARYIEDGLTCWLVCWGIPQSPLPRTLFRPIARDKI
                     "
     gene            complement(8565..>12324)
                     /locus_tag="PKJ16_09630"
     CDS             complement(8565..>12324)
                     /locus_tag="PKJ16_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47290.1"
                     /translation="AKVSLSGEAMSGIINSVIGTHMRSLPFSFEGYAEYARSFRAINT
                     FGKALIDDMESIDEVIGISLSDKDWILGSMPSIPTIFTQADRGLLYYSYFRDPSAPGT
                     LKGLGFPAASIGYDTKPGPYNVAEGHVDNSVQAKDSQVSLVFNFEFPGATNMIPVVTR
                     RLSSAAVDFSTLQYLEVWYRSGGGSGTVEMAFDIGKINEDSDGDGFMSTEDLNHNGVL
                     DADPAAGIFEDVGYLFNGNRRTRVGSGPRLNAVTIGDGVLNNEDLNGNGVLDTAELSV
                     ALPGEITAPSSVPPAGITVDLADTGWKQARIYLDKSVAAYTNNPLFYEAVLREVEAVR
                     LRLRRGTASAGSIFVDSVRFVSSRWKGTKIDGVPMEAPGQFKVSIVDTLSDPEYRNDS
                     FLMRQRSTFKSLHGERNDADIVREKESALQIEYNLANRRASVSRKYVKAMDLRFYKTM
                     NLWFNYREFGGGDIYRVRVGSSERDYLEYEFSPDAHRAWREITLRLNDSSGGRVRLAR
                     SVGNPDLKRIVLIEIEAVANGTGRFWVNDIYVSDPMVLKDDAHWYEAEIKFNRPLFYT
                     KSGVPVISDLNIKFIQKGHGANFSTVGKSNDEVREEYYGIFSSMKILPNWGLRADYIV
                     EETATDGLNEKVDEARRGNTSRKSFFLETNYASNIPAVPSVTVKYKQEDYANTRKEFV
                     ASVPFNRNTDRKTYTPTILVEEKLDRVLGGSLSASLFMDMAFREERIRRGGSGGDAVA
                     LSSAIALEESEKRQQGLTTLGMMYQNAVFYVNPRVEVAAHEIVELKGKSSLNDTRVLS
                     DVRGNFHAPFTDADDSKFVERNKKGSLALGVRKPMIVRPEMKIDMSYYENKFRDYDEA
                     ERLLAGKFSRSKDARGYVANRIDLPFFFTDSPGLKFIRSLNVSYLRSASVQESDVPYE
                     GEKTDAFQEEYGVDRVYNGISDLGLNLYRFPPWHFFNGRGTCANARDYVNERFNSPLY
                     YGDGAPATTYNNGVRLIDNLSYSGAVDFDSLSIVFGGSFNQVSERQMVYGIPQQVVTI
                     NQNITCNFDLMKMFTMGFFRPNTIGIPHHAAYVNATYGFTRNMFVTSNIEEDVHTHGI
                     GLTFKRDRASLSFKGTIDFRYRWDREYIPLDDADRDSRDDVYVANINAVVFHEIDKSY
                     TFSVVYETDVQWLYRLFSFIYQLTAYPIFSIEYAQTINRYDYSLTVSPEPYDLQLITG
                     KLTLDLHKNVQGGISTRYAIEKWLNRETQGVYKEVESYEIGFGFTLLF"
BASE COUNT         2525 a         3750 c         3615 g         2434 t
ORIGIN      
        1 gcatgtcgac ttctacaaga tcaccgccgg cgagaagatc cacacgcacg ttccggtcga
       61 gctcaccggc tcggcggagg gagagcgcat gggcggcgtc gtggagttcc tggagcgcga
      121 gctggagatc gagtgcctcc cggacgagat gccggaaaaa atcgtcatcg acataaccac
      181 gctcggtatc ggcaaatcca tccagcagaa ggacgtgccg acatccggat cgctgaaaat
      241 actgggcgac gcgcaccgcg tgatcgtggc ctgcgtggtg ccgcagaagg ccagcgagga
      301 ggcggcggaa cctgccgcgg aggtcgccga aaccaaggcg gcggaacccg agaaggaaaa
      361 gaagtcggaa tagtccgagg agcgttcgcc gcccgcgggc ggcgtaacat aaatttgaga
      421 atggccactc aagcgctata ctgcccgcag agcgtgagct gcaaggaagg ggtgtatcgt
      481 tgaaactgat tgtagggttt ggcaaccccg gtgagaagtt cgtcaataac cggcaaaaca
      541 tcggcttcaa ggtggtggag atcctcgcga acaacgagaa tatcgatgtt cgcgtcaaga
      601 agaagaagtc ggtgatcggc cggggctccg tgaacggaac cgaggtggtg ctgctcaagc
      661 cccaaacctt cgtgaacctc atcggcgagt cggtattgta tatcgcgtcc ttcctgcgga
      721 tcaacgtgcg ggagatcata tgcgtcctcg aggatccgac gcttcccgtg ggggagcttc
      781 gcgtggatta ccgcttgtcg aagatcaagc acgagggcat cgaatcgatc accaaagcac
      841 tcaagtccga caagttcgcc aaggtgcgcg tgggcgtggg cgtccctccc aagtccatga
      901 cggtggaaca gtacctgctg accgacttca ccgaccggga gaacctcatc ctgattgacg
      961 ttctcaacaa ggccgaggag gcggtcaagg cgttgatcac caacaccatc gaatcggtcc
     1021 aggaaaacta caatcccgag ggccccgtcc gcatagagcg ccgtcgcgtc cgcaaggtat
     1081 acgtaaagcg atagcgcccc ttccgtcccg gtccgccgtc cgttgcgccg acgaaccccc
     1141 tgaagaacga atgcagtcgc gtgttgctta taaggcagtg aagccgccat ggccgcactg
     1201 ccggaaaaaa aaccgcaagg gacagaaccg ttgaagggcg ggatagtgcg gagagtaact
     1261 gtgcggggtc aacgggcggg tgttctgtcc cttgatagtg caggattaaa caaggcctct
     1321 tatgcgatgg tacgcttgcg atacagatac aagagccggg aaagacagat ctggacctgg
     1381 acgatggtat ttcgatcggg aaggcgcagg tacacataga gtgtcgagtt atgtctcatt
     1441 ataaaacgct cccattgaca tgttctcctt tcggccaata cctatctcgc atgttttgta
     1501 tgatcgcatc gcgcctgtct agtgtaagca cctgcgatac ttcatcgatg ccgcttcacc
     1561 agatacaaac cgtatgattg caaaaccacc tacgaacaac aggccgaata gccgcacgaa
     1621 cggcagaaca ggcaccccga ctcgggtatt aacgcacatc cgcattcggg gcagtgctgc
     1681 attgcagtat gccgcttcga atcctggatc gacggttccg gacgggaaat ttgttgtttc
     1741 atatctttaa tcatacatta gtatagtata tatttgttta gtatttgtcc agcgattttt
     1801 ttattttttt taaaaaaatt actcccctga tggtattata ggtacaatga agaatctgct
     1861 tatgctatcc gacaccgaac gctgatcata tcaacacaca aggacggctg tttaaatgaa
     1921 aaaaccattg ttcgaccaaa gtggcgaatg ggaagaattg ctgaaaaacg atctcggcca
     1981 atccccgaag cccaatcccg aaacgaccgg cagcaagacc gtcggtgcca gcgcatcggc
     2041 ggggctcaat ttcgacatca agcccgagga gctcgagagc tacctgaacc gatatgtcat
     2101 gggccaggag tcggccatcg aggtgatcgc gaccaaggtg tgcacacact tcaatcgcat
     2161 gaagctcgag gcgacgatcc ccgaggacga gcgcatcgtg gggaacatca agagcaacat
     2221 gctgctcatt gggcccacgg gcgtggggaa gacctacatc atcaagctga tcgcgcgccg
     2281 catcggggtc cccttcgtga aggccgatgc gaccaagttc agcgagaccg ggtatgtggg
     2341 cggcgacgtc gaggacctcg tgcgggagct cgtccacgag gccgagggcg acatcaagaa
     2401 ggccgagtac ggtatcatct atctcgacga gattgacaag atcgcctcgg cggggacggg
     2461 acacggcccc gacgtatcgc gcaccggggt gcagcgcaac ctcctgaagc tcatggagga
     2521 ggcggaggtg gacctcaaga cgccgcacga cctcgcctcg caggtggagg cggcgatgga
     2581 ggcgcagcgc accggcaagg tcacgcgcaa gaaaatcaac acccggaaca ttcttttcat
     2641 cgtctccggc gccttcagca gcatcgagga tatcatccgc cgccgcctga acatgcaggc
     2701 gatcggtttc gacaaggcct atgcgtcaaa gacggacggg ccggcgctcc ttacgctcat
     2761 caagaccgag gacctgatgc agttcggctt cgaatcggaa ttcatcggcc ggttgcccgt
     2821 caccgtggtg ctcaacgagc tcgacgagga gggcctctat cgcatcctga agaaccgcta
     2881 cagcaccgtg gtattgggca agaaattcga ttttcggtca tacggcatcg agcttgattt
     2941 caccgacgac gccctgcgga cgctcgcccg ccgcgcgcac gcggaaaaaa ccggggcgcg
     3001 gggccttctc acggtgttcg agaaggtgct catcaagttc gaaaagacgc ttccctccac
     3061 cgagatccgc cagctcacgg tcgattgcga cgtggtggaa caacccgagg cggcgcttcg
     3121 aaagatgctg ctctccgaca gcatcaagag cttccagaag gacttcctgc tgaaaaacgg
     3181 catctatctc gagtttgatc gcgaggcggc cgaggcgctc catcggatgg cgggcgagcg
     3241 caactgcagc atcaagcagc tctgcgagga gctcttccat gattattcat acggcatcaa
     3301 gctgatggag caggacacct tccgcatcac gcgtgatgcc gtggagcggc ccgacgccta
     3361 tctcgaggaa ttcatccgga agaactaccc gaagggccgc ggcgcatcct gatggggccc
     3421 gagtttttta cttgattttt tttcgccaac acccatagca tgcaaagcat acaatatatc
     3481 ttagaggagg ttgcacaatg gaacagcttt ttgctcagga catgagcggc ggtggcggcg
     3541 gtggaagcat cgttaccacg ataatctatc tcatttttct tgtcgcgtgg attgcgggca
     3601 tgtggaaggt gttcgaaaag gccggcaaac cgggctgggc ggcgattgtc cccatctaca
     3661 acattatcgt actgctgcaa atcatcaaca aaccgctgtg gtggatcgta ctcatgctca
     3721 tccccatcgt aaactttatc attttcctca ttgtgagcat ggaattggcc gtttgcttcg
     3781 gcaagagcaa gggatggggc gttggccttc tcttcttctt tggttttgtc ggttatcccc
     3841 ttctcggttt tggggatgcg acctataccg cacctgccgg atcgtaagtt ctgtccttct
     3901 gttcgaacca aagccccccg cctggggggc tttttcgttt catcgccaca gcccgcggat
     3961 cctgtgtccc cgaaaacata ttgacaggcc gtctaaccgc tgtagcgtca tccagtctct
     4021 catgagtcga cccgtgccga ccacccacgg gaccgttccc gcttggagca tgtacaatgg
     4081 atttcgacct taaaaaattc atacgcgaca cccgcgcggt cctgctcacg ccgcgcgcct
     4141 atttttcgac tatggatacg accggcggat attgggagcc ggtgatccgg gccgggatct
     4201 acgggatcgt cgccggcgtg ttgcagctcg cctggcgcct gctgttcccg cagcccgtcc
     4261 ccgcgctcgc cggcgtagcg ggatcgaccg gcgtgagcat cgccccgttt atcacgccga
     4321 tcatcgcggt cctcatcggc ctcttcgcga gcgcgattct cctgctgatc gtctcggcga
     4381 tctgcggcgg cagcaccaat gtcgaagcgg cgatccgctg cggcgcggcg ctcacggcgg
     4441 tggcgcccgt aaacgcgctc ttcgggttcg tggccgcgat ccacccggcg gcgcgcgcgg
     4501 ccgtctcgtt cgtcatcacc ctgtacgcca tcgcgctcat gtaccatgcg ctggtgcgcg
     4561 cgctctcctg cgcggaaaaa ccggcgcgca tcgccgccgg catcatcgcc gcgatcccgg
     4621 cggtcgcctt cgccatcgcg ctcacctccg cgctgaaacc aaaggacgca atccagcagc
     4681 tcagcggcga ccggcagcag cgcgaggagc tcgagcgaac cgagcgcgag gcgcttgagt
     4741 ttattgatcg actgaaggag atgcagcggc gccagaaggg ggagtgaacg cgcggtcgcg
     4801 cagccactcg atgaggttcg ctcgaatgac gacgaggtcc tcgtctcgga cgagattatg
     4861 gtcgccatcg ggaatgttca cgagtctcgc ctctccgcgc acgcccgata cgatgcgtcg
     4921 cgcgttttcc gggtcgacca catcatcaca ttcgccgtga atgaccagca ccggcgccga
     4981 tattcccgga agcgcgccac gcggatcgag cgcaccgatc tccctgaaaa accggttatt
     5041 gatggcgatt ccctggacga cggtggcccc ctcctcggga aggcgatccg tgtcgcgcat
     5101 gccggtcatg cgatacagga ggcccggaag gtccaccggc gtggcgatga gcacgagcga
     5161 gtcgacgcta tccgcatgcc gggcggcata gaggaccgag accgtcccgc ccatgcttga
     5221 gccgatgaga tgcacgcgcg tgaaaccctg cccgcgcatg aagcgcacgg cgcaggcgag
     5281 atcgtccacc tcctgttcga tcgacaggtc ctcgatgcgc ccctccgatt cgcccacgaa
     5341 cgagaaatcg aacgcgagga gggtgagccc ggccgcgcgt atgtcgtcgg ccaggcgcgt
     5401 aatcttgtag ccgtccttgg tggagaaaag cccgtggcag aaaatcgcgc ctgcggacgc
     5461 aggcggatcg gcccgatgga ggcggccggc gatgcgctgg ttccgtccgt tgcgaaattc
     5521 gacgcgctcg gcggtcatcc ggatttttcg aggcgcatct ccggctcgaa gtagccgtcc
     5581 gaattgcgtc cgtcgatgat gatccgttcc ggcttgagga cgagggtcgg gcggccccgc
     5641 gagcggtcga ggccggcgtg aaaaatgaag tacgacgacg acgccttcac gaaaccgctc
     5701 tttaggttga tgtggcccgc gcttttttcg tttttcatgt cggagacgtt aaagcgtatg
     5761 cggtcgccct cctcgatcgt gtagacgccg cggaacacgt acgcgtgcga ccgcagatac
     5821 atgacgcaga tggcgcgata atttttcgtg aacgtgaact cgaacccgta ccggccggaa
     5881 tacatgagcc gccatcgccc ggcgatctca gcaaatccca ggggcctgcc cgccgccgaa
     5941 acagaggttg cgcacaacgc aatggccaca acggcggcgg acaccgacgc ccacaatgca
     6001 cttcggttat ttcccacgat acttcccctc ttcgaatcgt ccggtgaacc gcgtcccgtc
     6061 ccgggtggtg aaggtcccat agccgtgcat gcggccgtcc ttccattgtc cctgatagcg
     6121 ctcgccggtc ggccaggtgt attcgccgac gccgtttcga cggtcggcct tgaattcccc
     6181 caggtacgcc tcgccgccgg gatagcgata ctcgcccggc ccatcgcggc gatcgtcccg
     6241 ccacgacccc tcgaaggtcg agccgtctcg ccatcgatag cggccggcgc cctcccggcg
     6301 cccgtcgcgc gtatcgccct cgtacaccgg cgcgatcaca atgccgccct cccgtcggtc
     6361 gagctcgtag gcgctataca tgaggcggcc gcccttgaag acgagcgcgg gcgagcgata
     6421 ggcctgtatg ttttccagcg gatttgcgtc cagcaccgca aggtccgccc acttgcccgg
     6481 ctccacgctc cccattttat ccgccgcgcc aaggatcagc gcattgttgt aggtggcgca
     6541 gcgaaggacg tccttcgcgc tgatccccac gcgatgcatg agctcgagct cgcgccacag
     6601 cgccccgtga taactgaagg gcatgcccgc gtcggtaccg cacccgatga gcacaccggc
     6661 gcggaacatc ctcctgaggt tttcgggacc atgtcgcagc acgccgtaat acagctcggg
     6721 gttcgggagg aatttttttt cggcccgata gcgcccgcag tcgcggacgc ggaagagctc
     6781 catgttcgcc cggttgttcc gatggatgcg cgactgcaca taccgggagg ccagcgaatc
     6841 atagtacgcg cggttgattg cgatctcgtt cctggccgtt tccgttttga gcgtcgccgg
     6901 ctggtgctcg aaggcctcgc ccggcgagag gtagctcgcg atcaccatcg tgggaacgca
     6961 ggcgatcttt ttgctcgcca tcatcgccgc ctcgtcgtcg gagagcgcgg cgtcggagag
     7021 cgaatgctcg agcgaatgga gcggatagcg cgtgatccgg tcgaaaccgt acttgaagtg
     7081 attgtgcgcg atcaccggaa gccccttgcg ctccgcgaag gcgaagatat ggcgcaactg
     7141 ctcgtcggtg tacaccggga tctcgccgcg accgcagaac accgaccggt tgtccacggt
     7201 gagcttgata aaggacgcgc cgcgctcgac gttctgctcg agcttgtatt tcaggtcgga
     7261 aaggtcggtg aaatcgaccg ttacgctgcc ggtgataagg ctcgttggcc cgccgaaaat
     7321 cgacacgtcc gagggcctga tgtccggatg gctccctgcg atattgatga tagagttaca
     7381 gtagaacact cgcggcccga taagcccgcc gccggcgatg tcgtcgatcg cgcggtgcaa
     7441 ctcggacacc agcgcgccgg tatcgcgcac ggccgtgatc ccggcgcgga tgcactccgt
     7501 gtagttgcgg cgattctgct tcaggtcgtc atagagctca aagaagtcga agagcagggt
     7561 gcccggcatg gtgatatggc aatgggcgtc gatgagcccg gggatcacat acttgcctcc
     7621 gagatcgaag gtttcacccg gatgcgctgt tgcatctttc gtcgtaatgc tcgcgatcgt
     7681 atcgcgcatg atggtaacga ccgccccgcg acgcaccgtc ccggttttca cgtcaataac
     7741 agagcaattc acgagacgca gcggtttttc gtaattgacg cgcaggcgct cgaccggcgt
     7801 gcgcgccccg cagccgggca tgcccgatag cgcgatcgcc gccgtccctg cgagggcgcg
     7861 ccccaggaag tctcggcgcg tcatgcgcgc atcggtttca tcgtgtgcca tcattgcatc
     7921 cttgcgattt cgatcgcaca atcaaattac accatcggtt ttcggcaatc atattttatc
     7981 gcgcgcaatt ggccggaaga gggttcgcgg gaggggggat tgcgggatgc cccaacacac
     8041 cagccagcaa gtcaggccat cctcgatgta tcgggcgaga atgtaatttg gaaaacgata
     8101 gttatcgttt tttccatccc gacatgactg ctttacgaca tcatccagat attgtcgcgc
     8161 ggcttgggcc agaatcaacg agtccgacag cttgaagaaa ttccgcatgt cctttacata
     8221 atccgcctca tcctcccgga agcgcacatg gaccgtgcgc catcgcctca acgcatcccg
     8281 ccgttgatac cgtatcgccg atcccgtatt cggccgcttc cgtcgatcag ccatcgcgcg
     8341 ccgaaacagc atcacgatcg cccgcgacct cgagcacccc atgatcgcac atgcggaatc
     8401 aatcagttcg atggtcgtct catgaacgta gagcgtcgta tcgatcgcca tgtcatttcc
     8461 tccatttcgc gtcatcatag agaatacgaa atggaacggt gaatcatgaa cgaaattttc
     8521 ataccgtgcg gtccggacgg cccgctcacg cagggagaac gaaatcagaa caacagcgta
     8581 aaaccgaaac cgatctcata ggattcgact tccttgtaaa caccctgggt ctcccgattg
     8641 agccacttct caatcgcgta gcgcgtcgag atgcccccct gcacgttttt gtgcagatcc
     8701 agcgtgagct ttcccgtgat cagctggaga tcgtacggct cgggcgaaac ggtgagcgaa
     8761 tagtcgtagc ggttgatggt ctgtgcatac tcgatgctga aaatcggata tgccgtgagc
     8821 tgatagatga acgaaaaaag acgatagagc cattgaacat cggtttcgta gaccaccgaa
     8881 aaggtgtagc ttttgtcgat ctcgtgaaac acgaccgcgt tgatattcgc gacatatacg
     8941 tcgtcgcggc tgtcgcggtc cgcgtcgtcg agcggaatat actcgcgatc ccatcgatat
     9001 cgaaaatcga tggttccctt gaacgaaagg ctcgcgcgat cacgcttgaa ggtgaggccg
     9061 attccgtgcg tatggacgtc ctcctcgatg ttcgatgtca cgaacatatt ccgcgtaaac
     9121 ccatacgtcg cattgacata cgccgcatga tgcggaatgc cgatcgtgtt cggccgaaaa
     9181 aaccccatgg taaacatctt catgagatcg aagttgcagg tgatgttctg attgatggtc
     9241 accacctgct gcggaatgcc gtacaccatc tgccgttcgc ttacctgatt aaaggacccg
     9301 ccgaagacga tcgagagcga atcgaaatcg accgcaccgc tataggagag attgtcgatg
     9361 agccgcaccc cgttgttata ggtcgtggcc ggcgctccat caccgtagta cagcgggcta
     9421 ttgaagcgct cgttcacgta gtcgcgcgca ttggcgcagg tcccgcgccc gttgaagaag
     9481 tgccacggcg gaaaccggta aagattgagc cccaggtccg aaatcccgtt gtacacccgg
     9541 tccacgccgt attcctcctg gaaggcgtcc gtcttctcac cctcgtaggg cacgtcggac
     9601 tcctgcaccg aggcgctgcg cagatagctc acgttcagcg agcggatgaa cttgagcccg
     9661 ggagaatcgg tgaaaaaaaa ggggagatcg atacgattgg cgacatagcc gcgcgcgtcc
     9721 ttcgaacgcg agaacttccc cgcgagaagc cgctcggcct cgtcatagtc gcggaacttg
     9781 ttctcgtagt acgacatgtc gatcttcatc tccggacgca cgatcatggg ctttcgcacc
     9841 cccagcgcga ggctcccctt cttgttccgc tcgacgaatt tggaatcgtc cgcgtcggtg
     9901 aagggcgcgt gaaaatttcc ccgaacgtcc gagagcaccc gcgtgtcgtt gagcgacgat
     9961 ttccccttga gctccacgat ctcatgcgcg gcgacctcca cccgcggatt cacgtagaac
    10021 accgcatttt gatacatcat cccgagcgtc gtgaggccct gctgccgctt ctcgctctcc
    10081 tccaatgcga tcgccgagga gagcgcgacg gcgtctcctc cggaaccgcc gcggcgtatc
    10141 cgctcctcgc gaaacgccat gtccatgaac agcgacgcgc tcagcgagcc ccccagcacc
    10201 cgatcgagct tttcctccac caggatcgtg ggggtgtagg tcttgcggtc ggtgtttcga
    10261 ttgaacggaa cgctcgcgac aaactccttg cgggtgttcg catagtcctc ctgcttgtat
    10321 ttcaccgtca cgctggggac cgcggggatg ttcgacgcgt aattcgtttc aaggaaaaac
    10381 gatttccgcg aggtgttgcc gcggcgcgcc tcgtccacct tctcgttgag cccgtcggtc
    10441 gcggtctcct cgacgatgta atcggcccgc aggccccagt tgggcagaat tttcatcgac
    10501 gagaaaatgc cgtagtactc ctcccgcacc tcgtcgttcg atttcccgac cgtgctgaag
    10561 ttcgccccgt ggcccttttg gataaacttg atattgaggt ccgatatcac cgggacgccg
    10621 gacttggtgt agaagagcgg acggttgaac ttgatctccg cctcatacca gtgcgcgtcg
    10681 tccttcagga ccatcggatc gctcacgtag atgtcgttga cccagaagcg cccggtcccg
    10741 ttcgccaccg cttcgatctc gatcaacacg atgcgcttca ggtccggatt gcccacggac
    10801 cgcgcaaggc gcacccgccc gccggacgaa tcgttgagcc gcagcgtgat ctcgcgccag
    10861 gcgcggtgtg cgtccgggga aaactcgtac tcgagataat cacgctccga ggagcccacg
    10921 cgcacgcggt agatgtcgcc gccgccgaat tcgcgatagt taaaccacag gttcatcgtc
    10981 ttgtaaaaac gcaggtccat ggccttgacg tacttccgcg agaccgaggc gcgccggttc
    11041 gcgagattgt actcgatctg cagcgcgctc tccttctccc tgacgatgtc ggcatcgttg
    11101 cgctcgccgt gaagcgactt gaacgtgctc cgctggcgca tcagaaacga gtcgttccgg
    11161 tattccgggt cgctcagcgt gtccacgatg gaaaccttga actgcccggg cgcctccatg
    11221 ggcacgccgt cgatctttgt ccccttccag cgagaggaga cgaatcggac gctgtccaca
    11281 aaaatgctac cagcgctggc cgttccgcgc cgcaggcgca atcgcaccgc ctccacctcg
    11341 cgcaacacgg cctcgtaaaa gagcgggttg ttcgtatacg ccgcgaccga cttgtccaga
    11401 tagatccgcg cctgtttcca gcccgtgtcg gccaggtcca ccgtgatgcc cgccggaggg
    11461 accgacgacg gcgcggtgat ctcgccgggg agggcgacgg aaagctcggc agtgtcgagc
    11521 acgccgttac cgttgaggtc ctcgttgttg agcaccccgt cgccgatcgt caccgcattg
    11581 agccgcggcc cgctgcccac gcgggtgcgc cggtttccgt tgaacagata cccgacgtcc
    11641 tcgaagatgc ccgccgcggg atcggcatcc agcacgccgt tatgattgag gtcctccgtc
    11701 gacatgaaac cgtcgccgtc ggaatcctcg ttgatcttgc cgatatcgaa ggccatctcc
    11761 accgtgccgc tgccgccgcc cgagcggtac cagacctcga gatactgcag cgtggagaaa
    11821 tcgaccgccg cggacgacaa ccgccgcgtg accaccggga tcatgttcgt ggcgccgggg
    11881 aactcgaaat tgaacacgag cgaaacctgg ctgtctttcg cctgcacgga attgtccacg
    11941 tgcccctcgg ccacgttgta ggggccgggc ttggtgtcgt atccgatcga cgcggcgggg
    12001 aagccgagcc ccttgagggt cccgggcgcc gacgggtcgc gaaagtacga ataatacagc
    12061 aggccccgat cggcctgtgt gaagatcgtc ggtatcgacg gcatcgaccc gagaatccag
    12121 tccttgtccg aaagcgagat cccgatcacc tcgtcgatcg actccatgtc gtcgatgagc
    12181 gccttgccga aggtgttgat ggcgcggaag ctgcgcgcgt attcggcata cccctcgaag
    12241 gagaacggca gtgaacgcat gtgcgtgccg atgacgctgt tgatgatgcc gctcatcgct
    12301 tcgccgctca gcgagacctt cgca
//
LOCUS       DAORHF010000098        12312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4257_length_12312_cov_5.123848, whole genome shotgun sequence.
ACCESSION   DAORHF010000098 DAORHF010000000
VERSION     DAORHF010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4257_length_12312_cov_5.123848"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..3473
                     /locus_tag="PKJ16_09635"
     CDS             225..3473
                     /locus_tag="PKJ16_09635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47291.1"
                     /translation="MRTVVQCLHILLAVLMGAALVAGCARQGEVRAYPAIESGVLDLR
                     DWDFDRDGPVRLNGKWAFYWDRLLAPADFARGTIGPTGYYPVPLYWTKYEGLGLPGRG
                     MATYRLTVLAKGAIGEIAIKTPELYTEYRLWVNGAIVDEHGTVAGTGIRFLKPNVFAL
                     NVAGSAIDIVLQIKNEAHGNAGIGQNFVIGTAADLFAKRYSVLILEVALSAICFFLGC
                     HHLVLFSFRRAARELLFFGLFCVAIAMRTLLTGETFVMTLLPDFPFAIASRLSTSSIP
                     LMAITFIFFAGALFPALARRAVVYAIAGVHGLYLVMIWTTPTYWYSYAFQHYLHAVTL
                     CMLVILYWVVRSIIAGNRLSYMFLVACGVLFVGAYNDMLFYQQLVDTGYYLSLGFTGF
                     IIIESVMLGIRFSQEYATIRRLSDRLQAVDSLKDEFLANTSHELRTPLNGIIGITESL
                     IDGATGPLPENTVRNLNLIVSSGRRLGMLINDILDFSKMRHDEIVLNRRPTDLRAIVD
                     LVLLTSRSLLKSKPVQMFNRIPADIPLVFADENRLQQVLYNLVGNAIKFTHAGEIAVS
                     ARVEAGAPGADMVVVTVSDTGIGVPPDEHDAIFESFRQADGSTARLYGGTGLGLAITR
                     TLVELHGGTIGVESEHGKGAAFSFSLPVATGVADGEIVASETQGIMARVAAVRDALVE
                     DPDALDATPQRATDAEAKGKILVVDDDAVNQKVLANHLLLHGYEVRLASDGFQALELV
                     DGEGGFDLVLLDVMMPRLSGYEVCQRIRERHAASELPVIMLTAKNLLVDLIKGLDTGA
                     NDYLGKPFNKRELIARVRTHISLKKASQEARRLYGIDREMKIAREIQMSIVPESLPAV
                     PGLCCAARYLPMAGVGGDLYCVDVTDDGAFAVLFTDVSGHGVPSALIASMVKIAFEVQ
                     KPLAARHDCYLQELNRILMPNIRPQFLTACYLYIEPAERRLHYSNAAHPPLLIHRRET
                     GELLSFKPRGKLLGVVDDIACSSEEIPLRTGDRLVLYTDGITEALEAGSDKKLKDVEA
                     HMFGTEAFTAAIRSGAERPAGEFADHLLAELERWTGTNQFNDDVTLMVIDVE"
     gene            3625..4578
                     /locus_tag="PKJ16_09640"
     CDS             3625..4578
                     /locus_tag="PKJ16_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005220756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HNV47292.1"
                     /translation="MNIVFLDAATLNFGDMDMGPIAACGDYREHCLTAAHETAARLAG
                     AEVAITNKVVVDRAVLDACPALRFVAVAATGYNVVDVDAARERGIGVANVPGYSTESV
                     AQLAMAFILALSTRLMEHDRAARGGAWERSPMYTMGDWPWRELAGATVGIMGLGAIGA
                     RVAELCRAFGMRIVALGREGVSYNGTVERVGLDELAARSEYVSIHMPLTDYSRRLADA
                     RFLGMMKPTAYLVNLARGPIVDAHALAEALRAGRIAGAAVDVRDVEPPPADDPLLGAP
                     NLIATPHVAWASVEARRRLIAEIAANIRAYRDGAPRNIVNS"
     gene            4803..5102
                     /locus_tag="PKJ16_09645"
     CDS             4803..5102
                     /locus_tag="PKJ16_09645"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HNV47293.1"
                     /translation="MEKPVSNMNIEIKVDEHDAVGKFTNFANITHTPEEFIVDFLFVN
                     PVPPPGFGKLMARLVMTPGHAKRILGALSENIRNYEEHFGEIRIAQFPGGPDSVQ"
     gene            5201..5371
                     /locus_tag="PKJ16_09650"
     CDS             5201..5371
                     /locus_tag="PKJ16_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47294.1"
                     /translation="MPYKITDDCTSCGACASECPVEAIKEGKNKYVINQDECTDCGSC
                     VDACPADAIVEA"
     gene            5473..5964
                     /locus_tag="PKJ16_09655"
     CDS             5473..5964
                     /locus_tag="PKJ16_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47295.1"
                     /translation="MRTLVLIIAVTLGISAPPAGGNDAQRLAEASRNYLKLEKIYMHW
                     NEPWFRKGGYYRQVMYDYLVDLIGKEIHTDHTFMLVSPESIYRQEVQCQVRSGRWLHI
                     VGKFSIADIKKIIGDDIGLLKGWWRSRKMIAVYGKVRRFRIDETETRKEIVLYLETVH
                     VRE"
     gene            6087..7922
                     /gene="glmS"
                     /locus_tag="PKJ16_09660"
     CDS             6087..7922
                     /gene="glmS"
                     /locus_tag="PKJ16_09660"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HNV47296.1"
                     /translation="MCGIVGYVGNESALDVILSGLKRLEYRGYDSSGVALVNGNMFIE
                     KRVGKISNLEDHLAGKNLGGFHVGIGHTRWATHGVPSDINAHPHTGCHERIAIVHNGI
                     IENYAAIKKMLIDKGHIFSSETDSEVIAHLIEYYIENNSFLDAVLKALAKLEGTYGIL
                     AISESEPDTIIAARKGSPLLLGIADDAMILASDASAIIEYTRKVVYLEDGEIIEVKKD
                     KMKTFDLERRRIDKKVEDITWDISDIEKQGFEHFMLKEIFEQPETIQNAYRGRIVIDE
                     GLVRLDGITLSRQDINDVRRVILIACGTSFHAALVGEYLIEEFARIPVEVEYASEFRY
                     RRPILLRGDLIVVISQSGETADTLAALREARDRGANVVGITNVVGSTIARECNAGGVY
                     LHAGPEIGVASTKAFTSQIVVLYLLGIFLARRKGMTADEAGALLRELTKIPDKVQWIL
                     DRADAVKEVAELYKDTANCLYLGRGINFPVALEGALKLKEISYVHAEGYPAAEMKHGP
                     IALIDENMPAVFAAPFWDEVYAKVVHNMQEVKARSGKIICIATEGDQEILKHADHVLR
                     IPNIGKMLSPLLSVIPLQLLSYYVAVLRGCNVDQPRNLAKSVTVE"
     gene            8075..8449
                     /locus_tag="PKJ16_09665"
     CDS             8075..8449
                     /locus_tag="PKJ16_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV47297.1"
                     /translation="MIIIENIDHVGLTVSSLDKSIQFFKELFDFEIVERITNANQAFI
                     RVGDIILCLNEVSGYRCPEETKNHISFYIDEEDFDDAVDELGEREVPIVFGPENLRKG
                     QSVVFLDPDGNRIELCYPRMNP"
     gene            8446..9102
                     /locus_tag="PKJ16_09670"
     CDS             8446..9102
                     /locus_tag="PKJ16_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HNV47298.1"
                     /translation="MTPEDAFRRARVIPVAVLDDAAAAVDLAALLVRHSLPLIEVTLR
                     TSASRDCIEAIAARVPEICVGCGSVLSPDDLDAAMDAGARFAFAPCLDEAVLAHAARR
                     GLPFAPGVATPTELGRALALGCALVKVFPAAPLGGPGFVRALCAPFATRTPRVVPTGG
                     VSEEAIADYLAIPEVVAVGATFCADPALVARRDFDELERRIMRVRRAIDGDRDRRIHA
                     "
     gene            9095..10054
                     /locus_tag="PKJ16_09675"
     CDS             9095..10054
                     /locus_tag="PKJ16_09675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV47299.1"
                     /translation="MRDERAATDLLGRALDRRADLHANSRTTCYRIFNGGGDGIDGLC
                     VDRYGEYLLVQLYEPSLYDAIDVIRASLETIAARTPFPVAGVLAKDRTLVRETVEIAE
                     RRRSVLLLGERPPEDYVVLQNGLRAAVDLLHGQNTGVFLDMREVRDALAQVYAETHDL
                     INLFCYTGLFSVHALAHGVSRAVNVDLSRPFLRRARRNYALNGLPVDDRDFICGDSFE
                     WSRRFSRQERRFDLVVFDPPTFSRNKRKTFSVKRNYRQALAALDMIAGRYALTAVNSQ
                     TVCEDEYRAFHPPGWKCLFVRHEPPDFRLDGAPYLKAGLWHTR"
     gene            10178..10936
                     /locus_tag="PKJ16_09680"
     CDS             10178..10936
                     /locus_tag="PKJ16_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47300.1"
                     /translation="MLYLSIALIAAGVLFILYSLMSSKHAPTARAGREPLETGPRDSD
                     ADALRDVLPPRPVPASERIDAPHDVRERHPLDDMRCSHEWTSGADARQDAPRDQPANE
                     AGLAHGSDAGATDAAGSTDAPASRAGADDPASSTDAADGAAYAVLYEDASNVIDYYAG
                     DTAIDPSLAAYSKIRRVGRGALEQARDGVNFYIGKKFYRYDFYRIADLRAGSGYLALS
                     LKDSDTVRLFIFERGGAIASEIIIRYNEYKKGQV"
     gene            10939..11934
                     /locus_tag="PKJ16_09685"
     CDS             10939..11934
                     /locus_tag="PKJ16_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47301.1"
                     /translation="MYYRYKSKKRDNKLVKFLFIAVCLCVIIFLGYTYRSHLMFWKYS
                     YNKLYAKISRIQAVKDPALRRTQLVELTKQCDGAKTDNEIDPDTFFLAGRAHYQLGEE
                     LVNRSFTRMVIDDALTVHDAAARREFMQAIKDIRKGFALLKKDDVTENHLYLARAMLY
                     LNFASPNELYALVQRYDTDRLKGMPEQARVLATLAVMAGAVEPGLAIMRGYGAPEGVE
                     GLLFLAALERTGKRYTSAIGEYKKVLQSSQDAAALKNAHANLGRIYYIQALYRESADH
                     FEHALMLDKNDAAVKVQAGKAHAALGNKARATALWTEALAADAANEEVKKLLGAR"
     gene            12031..>12312
                     /locus_tag="PKJ16_09690"
     CDS             12031..>12312
                     /locus_tag="PKJ16_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013450491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HNV47302.1"
                     /translation="MIFDKFYSMFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVACQT
                     STGKVLAVGHEAKRMLGRTPGDIVAIRPMKDGVIADFETTEKMIRYFITK"
BASE COUNT         2252 a         3909 c         3897 g         2254 t
ORIGIN      
        1 acacagagga gtctcgctga tgctctccac acctcgcgaa aaaaatctgg ccatccggtt
       61 ttattattcg cattggagca aaaaaacact tgtcatcgtt ggtaaactgt ttatgttttt
      121 agtatgatat gtgatgcggc atgatgttct cacgtcatac acgaaatcga caatccgaac
      181 cgtaccggcg cgctcggcaa caaggccgtg ccggatcggc atgcgtgcga acggttgtac
      241 aatgcctgca catcctcctc gcggtgctga tgggcgcggc gcttgttgcc ggctgcgcac
      301 ggcagggcga ggtccgcgcc tatccggcga tcgaatcggg cgttctcgac ctgcgtgatt
      361 gggacttcga ccgggacggg cccgtgcgcc tgaacgggaa gtgggccttc tactgggacc
      421 ggctcctcgc gccggccgat ttcgcccgcg ggaccatcgg gcccaccggc tactatccgg
      481 tgccgttata ctggaccaag tacgagggcc tggggctgcc cggccgcggc atggcgacct
      541 accggctgac ggtgctcgca aagggcgcca tcggcgagat cgcgatcaag acgccggagc
      601 tgtacacaga gtaccgcctc tgggtgaacg gcgccatcgt ggacgagcac ggtactgtcg
      661 cgggcaccgg gatccgtttc ctcaagccga acgtcttcgc gctcaatgtc gccggcagcg
      721 cgatcgacat cgttctgcag atcaaaaacg aggcgcacgg caatgccggg atcggccaga
      781 acttcgtcat tgggacggcc gcggacctgt tcgccaagcg ctactccgtg ctcatcctcg
      841 aggtggcgct ctcggcgatc tgcttcttcc tggggtgcca tcacctggtg ctcttctcgt
      901 ttcgacgcgc ggcgcgcgag ctgttgttct tcgggctctt ctgcgtcgcg atcgccatgc
      961 gcacgctcct caccggcgag accttcgtga tgacgctgct gcccgacttc cccttcgcga
     1021 tcgcgtcgcg tctctcgacc tcatcaatcc cgctcatggc catcaccttc atcttcttcg
     1081 ccggggcgct gtttccggcg ctcgcacgcc gggccgtcgt atacgcgatc gcaggggtgc
     1141 atgggctcta tctggtcatg atctggacga cgccgaccta ctggtattca tacgccttcc
     1201 agcattatct gcacgcagtc acgctttgca tgctggtgat cctctactgg gtggtgcgct
     1261 cgatcatcgc cggcaatcgc ctctcgtaca tgtttctggt cgcctgcggc gtcctcttcg
     1321 tgggggccta caacgacatg ctgttctatc agcagctcgt cgacaccggc tactaccttt
     1381 cgctgggctt cacgggattc atcatcatcg agtcggtcat gctgggcatc cgcttctcgc
     1441 aggagtacgc caccatccgc aggctctcgg accggctgca ggcggtggac tcgctcaagg
     1501 acgagtttct cgccaacacc tcgcatgagc tgcgcacgcc cctgaacggc atcatcggca
     1561 tcaccgagtc gctcatcgac ggggcgacgg ggccgctgcc ggaaaacacg gtgcgcaatc
     1621 tcaatctcat cgtctccagc gggcgtcggc ttggcatgct catcaacgac atcctcgatt
     1681 tctcgaagat gcggcacgat gagatcgtcc tcaatcgccg accgaccgac ctgcgcgcca
     1741 tcgtggacct ggtgctgctc acctcgcgca gtctcctgaa gtccaagccc gtgcagatgt
     1801 tcaatcgcat ccccgccgac atcccgctcg tcttcgccga cgagaaccgg ctccagcagg
     1861 tcctctacaa tctggtgggc aacgccatca agttcaccca tgccggcgag atcgcggtgt
     1921 cggcgcgcgt cgaggcgggc gcgcccggcg ccgacatggt cgtcgtgacc gtctcggaca
     1981 cggggatcgg cgtcccgccc gatgagcacg acgccatctt cgagtccttc cggcaggccg
     2041 acggctcaac cgcgcgcctc tacggcggca cagggctggg gctcgcgatc acgcgcacgc
     2101 tggtcgaact ccacggcggg accatcggcg tggaatccga gcacgggaag ggggccgcgt
     2161 tctccttctc cctgccggtc gccacggggg tcgcggacgg ggagatcgtc gcctccgaaa
     2221 cgcagggcat catggcccgc gtggccgccg tgcgcgacgc gcttgtcgag gatccggacg
     2281 cgttggacgc aacgccgcaa cgcgcgacgg acgccgaggc gaaaggaaag atcctggtcg
     2341 tcgacgacga tgcggtgaac cagaaggtgc tggccaacca cctgctgctg cacggctacg
     2401 aggtgcgcct ggcctccgac ggcttccaag cgctcgagct cgtcgacggc gagggcggct
     2461 tcgacctggt gcttctggac gtgatgatgc cgcgcctctc gggttacgag gtgtgccagc
     2521 gcatccgcga gcgacacgcc gcaagcgagc tcccggtgat catgctcacg gccaagaacc
     2581 ttctggtgga cctcatcaag ggcctggaca ccggcgccaa cgactacctc ggcaagccgt
     2641 tcaacaagcg cgagctcatc gcgcgggtgc gcacgcatat ctccctcaag aaggcctcgc
     2701 aggaggcgcg tcgcctctac ggcatcgacc gcgagatgaa gatcgcgcgc gagatacaga
     2761 tgtccatcgt gcccgagtcg ctccccgcgg taccggggct ctgctgcgcc gcgcgctatc
     2821 tgcccatggc cggcgtcggg ggcgatctct actgcgtcga cgtgaccgac gacggcgcct
     2881 tcgcggtcct gttcaccgac gtgtccggcc acggcgtgcc ctcggccctc atcgccagca
     2941 tggtcaagat cgcattcgag gtccagaagc cgctcgcggc gcggcacgac tgctatctgc
     3001 aggaacttaa tcgcatcctc atgcccaata tccgtccgca attcctcacg gcctgctatt
     3061 tatacatcga gccggccgag cggcggctcc attattccaa tgcggcgcat ccgccccttc
     3121 tgatccatcg tcgggaaacg ggcgagctgc tttcctttaa gccccgtggc aagctcctgg
     3181 gggtggtcga tgatatcgcc tgctcgtcgg aagagatacc gctgcggacg ggcgaccggc
     3241 tggtcctgta caccgacggc atcaccgagg cgctcgaggc cgggagcgac aaaaaactga
     3301 aggacgtcga ggcgcacatg ttcggtaccg aggccttcac ggcggcgata cggtccggcg
     3361 ccgagcgccc cgccggggag ttcgccgatc atctgctcgc cgagctcgaa cgctggaccg
     3421 gcacgaacca gttcaatgac gacgtgacgc tcatggtgat cgacgtcgag tgattggcat
     3481 gatccgcccg cttcactgtc gattgagcgc gtacggtggg ccatgtgaag acccatttcg
     3541 tgaagacgat cacgatgatg ctcgcgcacg atcgtgcgtc ggggactccg gccgtacgct
     3601 cgattcgtag agggggtaac cagcatgaat attgttttcc tcgacgcagc gacgctgaac
     3661 ttcggcgaca tggacatggg gcccatcgcc gcctgcgggg actatcgcga gcactgcctc
     3721 accgcggcgc atgagaccgc cgcccgtctc gcgggcgccg aggtcgcgat caccaacaag
     3781 gtcgtcgtcg accgcgcggt gctggacgcc tgtcccgccc tgcggtttgt cgccgtcgcc
     3841 gcgaccgggt acaatgtcgt ggatgtcgat gccgcgcgcg agcgcgggat cggcgtggcc
     3901 aatgtccccg gctactcgac cgaatcggtg gcgcagctcg ccatggcgtt catcctcgcc
     3961 ctctcgacgc ggctcatgga gcacgatcgc gccgcgcgcg ggggcgcgtg ggaacgctcg
     4021 cccatgtaca ccatgggcga ctggccgtgg cgcgagcttg ccggcgcgac ggtcggcatc
     4081 atggggctgg gggccatcgg cgcgcgcgtc gcggaactct gccgcgcctt cgggatgcgc
     4141 atcgttgcgc tggggcgcga aggcgtatcg tacaacggga ccgtggagcg cgtcgggctc
     4201 gacgagctcg cggcgcgctc ggagtacgtg tctatccaca tgccgctcac ggactattcg
     4261 cgccgcctgg cggacgcgcg ttttctcggg atgatgaagc cgacggcgta tctcgtgaac
     4321 ctggcgcgcg gtcccatcgt ggacgcgcat gcgctcgcgg aggcgctgag ggcgggacgc
     4381 atcgccggcg ccgccgtcga cgtgcgtgac gtcgagccgc cgcccgcgga cgatccgctc
     4441 ctcggcgcgc ccaacctcat cgccaccccg catgtcgcgt gggcgagcgt ggaggcgcgc
     4501 cggcggctca tcgccgagat cgcggcgaac atccgggcct accgggacgg cgcgccgcgg
     4561 aacatcgtga actcctgatg cgcgccgttg cggcccgccc tcgcggcgtg atccccctta
     4621 cggcgatgcg gtcgaccggc cgcaccggcg ctgcgtttcg gatcagggca tggagcggaa
     4681 ctggatcatc gtctggatca ttccagcgca ctcgcggttt ttcaattgct tttttctccc
     4741 gcgatgcggg aaatgcgcca tcggcggcgt gacccgcatc ggccgaaaca acagggggcc
     4801 caatggaaaa acccgtcagc aatatgaaca tagagatcaa ggtggacgag cacgacgcag
     4861 tcggcaaatt cacgaatttc gcgaacatca cccatacccc ggaggagttc atcgtcgatt
     4921 tcctcttcgt caatcccgtg ccgccgcccg gcttcggcaa gctcatggcg cgcctggtga
     4981 tgacgccggg ccacgccaag cgcatcctgg gcgcgctcag cgagaacatt cgcaactacg
     5041 aggagcactt cggggagatc cgcatcgcgc agttccccgg ggggcccgat tccgtgcagt
     5101 gacgcgacgc gcaatttttt tgcttgcgcg ctgggcggcg ctttcggtag tgttcgtttt
     5161 tgcgttacca gcgatcaata acgaaaatct ggaggtaccc atgccataca agatcaccga
     5221 tgactgcacc agctgcggcg cctgcgcgag cgagtgtccg gtcgaggcca tcaaggaagg
     5281 caagaacaag tatgtcataa accaggacga gtgcaccgac tgcggctcct gcgtggacgc
     5341 ctgtcccgcc gacgccatcg tcgaggcgta gtcgtcacga gcgctacggg gttcacgatg
     5401 cgcgcttcga cggcccgtcg tgaccttcgt ggcgtcgtcg cgcgcccccg cgcgcgccac
     5461 taagaaacgc aggtgcgcac cctcgtttta atcattgccg tcacgcttgg catctccgca
     5521 ccgccggccg gcgggaacga cgcgcagcgg ctggccgagg cctcccgcaa ctacctcaaa
     5581 ctcgaaaaaa tctacatgca ctggaacgag ccctggtttc gcaagggcgg ctactaccgg
     5641 caggtgatgt acgactacct ggtggacctc atcgggaaag agatccacac cgaccatacc
     5701 ttcatgctgg tctcgcccga atcgatctac cgtcaggagg tgcagtgtca ggtgcgcagc
     5761 ggacgatggc tgcacatcgt cgggaagttt tcgatcgccg atataaaaaa gatcatcggg
     5821 gacgatatcg gcttgctcaa gggctggtgg cgctcccgga agatgatcgc cgtgtacggc
     5881 aaggtgcgca ggttcaggat cgacgagacg gaaacgcgca aggagatcgt gctgtacctc
     5941 gagaccgtgc atgtccggga gtagaaggcg gttgggcgaa aaatgattgc attatttcca
     6001 tggatgcgaa atcagggtat cgatggcctt gccaagggac gctccccgcg cgggagcttt
     6061 tttattggcg ggggccggga gggaatatgt gcggcatagt cggctacgtg ggtaatgaga
     6121 gcgcgttaga cgtcatcttg agcgggctca agcgactcga gtaccggggg tacgattcat
     6181 cgggcgtggc gttggtgaac gggaacatgt tcatcgagaa acgggtggga aagatctcca
     6241 atctcgagga tcacctggcc gggaaaaacc tcggcggctt tcacgtcggg atcggccaca
     6301 cgcgttgggc cacgcacggg gtgccgtcgg acatcaacgc ccacccgcac accggttgcc
     6361 acgaacgcat cgccatcgtg cacaacggca tcatcgaaaa ctacgccgcg atcaagaaga
     6421 tgctcatcga caagggccat atcttctcca gcgagaccga cagcgaggtc atcgcccacc
     6481 tgatcgaata ctacatcgaa aacaactcgt tcctcgacgc ggtcctcaag gcgctcgcga
     6541 agctcgaggg gacctacggc atcctggcca tctccgagag cgagcccgac accatcatcg
     6601 ccgcgcgcaa ggggagcccc ctgctgctcg ggatcgccga cgacgcgatg atcctggcct
     6661 ccgacgccag cgccatcatc gagtacacgc gaaaggtggt ctacctggag gacggtgaga
     6721 tcatcgaggt gaagaaggac aagatgaaga ccttcgacct cgagcgacgc cgcatcgata
     6781 aaaaggtcga ggacatcacc tgggacatca gcgacatcga gaagcagggc ttcgagcatt
     6841 tcatgctcaa ggagatcttc gagcagcccg agaccatcca gaacgcctat cgcgggcgga
     6901 tcgtgatcga cgaggggctg gtgcgcctgg acggcatcac cctgagccgc caggacatca
     6961 acgacgtgcg gcgcgtgatc ctcatcgcct gcgggacctc gttccatgcg gccctggtgg
     7021 gcgagtacct catcgaggag ttcgcgcgca ttccggtgga ggtcgagtac gcatcggagt
     7081 tccgctaccg gcggcccatc ctcctgcggg gcgatctcat cgtcgtcatc agccagtcgg
     7141 gggagacggc cgatacgctc gcggcgctcc gtgaggcgcg tgaccggggc gcgaacgtgg
     7201 tcggcatcac caacgtggtg gggagcacca tcgcgcgcga gtgcaacgcc ggcggcgtgt
     7261 atcttcacgc ggggccggag atcggcgtgg cctccaccaa ggccttcacc tcgcagatcg
     7321 tggttctcta tctcctgggg atcttcctcg cgcggcgcaa gggcatgacc gcggacgagg
     7381 ccggcgcgct tctgcgcgag ctcaccaaga tccccgacaa ggtccagtgg atactggacc
     7441 gcgccgatgc ggtgaaggag gtcgccgagc tgtataagga tacggccaat tgcctgtatc
     7501 tcgggcgagg gatcaatttt cccgtcgcgc tcgagggcgc cctcaagctc aaggagatat
     7561 cctacgttca cgccgaggga tatcccgccg ccgagatgaa gcacgggccc atcgccctca
     7621 tcgacgagaa catgcccgcg gtgttcgccg cgccgttctg ggacgaggtg tacgcgaagg
     7681 tggtgcacaa catgcaggag gtcaaggcgc gcagcgggaa gatcatctgc atcgcgaccg
     7741 agggcgacca ggaaatcctg aagcacgccg atcacgttct gcgcataccc aacatcggga
     7801 agatgctctc gccgctgttg tcggtcattc cgctgcagct gctctcatat tacgtcgccg
     7861 tgctcagggg gtgcaacgtg gaccaacccc gaaacctggc caagagcgtg acggtggagt
     7921 agcgtttttg caactttttg cgaaccgggg cgcgattagt attgacagcc gccccgtcgc
     7981 gattacagtt taaccgtaca cgaccggggc atgaccaatc attcacgggc ggcggtgcgc
     8041 gatgccgcat catatctgag tatgagggaa tagtatgata attatagaaa acatcgatca
     8101 tgtggggctc accgtttcca gcctcgataa gtccatccag ttctttaaag agctcttcga
     8161 tttcgagatc gtcgagcgca tcaccaacgc caatcaggcg ttcattcggg tgggcgacat
     8221 cattctgtgc ctgaacgagg ttagcggcta ccgctgcccg gaggaaacga aaaaccatat
     8281 cagcttctac atcgacgagg aggacttcga tgacgccgtc gacgagctcg gcgagcgcga
     8341 ggtgcccatc gtctttggcc ccgagaacct ccggaagggc cagtcggtcg tctttctcga
     8401 tcccgatgga aatcggatcg agctgtgcta tccccgcatg aacccgtgac acccgaggac
     8461 gcctttcggc gcgcgcgcgt gatccccgtg gcggtgcttg atgacgccgc cgccgcggtc
     8521 gatcttgccg ccctcctcgt ccgccattcc ttgccgctca tcgaagtgac cctgcgcacg
     8581 agcgcatcgc gcgactgcat cgaggcgatc gccgcgcgcg ttcccgaaat ctgcgtcggc
     8641 tgcgggagcg tgctttcccc ggacgatctc gatgccgcca tggacgccgg agcgcgcttc
     8701 gccttcgcgc cctgcctcga cgaggcggtc ctcgcgcatg cggcgcgtcg cgggcttccg
     8761 ttcgcgccgg gcgttgccac gcccaccgag ctcgggcgcg cgctcgcgct cgggtgcgcg
     8821 ctggtcaagg tgtttccggc cgcgccgctg gggggccccg gtttcgtgcg cgcgctctgc
     8881 gccccgtttg cgacgcggac gccgcgggtg gtccccaccg gcggcgtctc cgaggaggcg
     8941 atcgccgact atcttgcgat acccgaggtc gtggccgtcg gggcgacctt ttgcgccgat
     9001 cccgcgctcg tcgcgcggcg cgatttcgac gagctcgagc ggcgcatcat gcgcgtgcgg
     9061 cgggcgatcg acggggaccg cgatcgacgt attcatgcgt gacgaacgag cggcaaccga
     9121 tctcctcggg cgcgccctcg accgtcgtgc cgatcttcac gcgaattccc gcaccacctg
     9181 ctatcgaatt ttcaacggcg ggggcgatgg gattgacggc ctgtgcgtgg accgttacgg
     9241 tgaatacctg ttggtccagc tctacgagcc ctctttgtac gatgcgatcg acgtcatccg
     9301 cgcttcgctg gaaacaatcg ccgcgcgcac gcccttcccg gtcgccgggg tcctcgcgaa
     9361 ggaccggacc ctggtgcgcg agaccgtcga gatcgccgag cgccgccgca gcgtgctcct
     9421 cctcggggag cgcccgcccg aggactatgt ggtgctccag aacgggctgc gcgcagccgt
     9481 ggaccttctg catggccaga ataccggcgt ctttctcgat atgcgcgagg tgcgcgatgc
     9541 gctagcgcag gtgtacgccg agacgcacga tctcatcaac ctcttctgct atacggggtt
     9601 gttttccgtg catgcgctgg cgcatggcgt gtcgcgcgcc gtgaacgtgg acctctcccg
     9661 gccgtttctg cgacgcgccc gacgcaatta tgccctcaac ggcctcccgg tcgacgatcg
     9721 cgatttcatc tgcggcgatt cgttcgagtg gtcgcggcgc ttctcgcggc aggagcggcg
     9781 gttcgacctc gtcgtgttcg atccgcccac attctcgcgc aacaagcgca agaccttttc
     9841 ggtgaaacgg aactatcgtc aggcgctcgc cgcgctcgat atgatcgcgg ggcgatacgc
     9901 gctcaccgcg gttaactccc agacggtatg cgaggatgag tatcgcgcat tccacccgcc
     9961 cgggtggaaa tgcctgttcg tccggcacga accgccggac tttcggttgg acggggcgcc
    10021 ctatctcaag gcgggcctct ggcacacgcg atgaaaaatc cttgacagcg cgctcgtcgc
    10081 cgcgaatggt cagaggagga ttgatggccc tcgggttccc cgcgagcgaa ctgatcccac
    10141 acgcaatcac aacgaaaccg gaatgggaag tggtgctatg ctgtatctgt ccatcgcatt
    10201 aatagcggcg ggggtgctgt ttatcctgta ttccttgatg agttcgaaac atgccccgac
    10261 ggctcgtgcg ggtcgcgaac cgttggagac gggcccgcgg gacagcgatg ccgatgcgct
    10321 gcgcgacgtt ctgccgccgc gcccggtccc cgcgtccgag cggatcgacg cgccccacga
    10381 tgtgcgcgag cgccatccgc tcgacgacat gcgctgttcg catgaatgga cgtccggcgc
    10441 cgatgcgcga caggatgcgc cgcgtgatca gccggccaat gaagccggtc tcgcgcacgg
    10501 gtccgatgcc ggagcgactg acgccgccgg ttcgaccgat gcgccggcgt cgcgcgcggg
    10561 tgcggatgat ccggcgtcat cgacggacgc cgcggatggc gccgcctatg cggtgctcta
    10621 cgaggacgcc tcgaacgtga tcgactatta cgccggcgac accgccatcg atccatcgct
    10681 ggcggcgtat tcgaaaatca ggcgggtggg ccggggcgcg cttgaacagg cgcgcgacgg
    10741 cgtcaatttt tacattggga agaagttcta tcgatacgat ttctatcgga tcgccgacct
    10801 ccgcgcggga agcgggtatc ttgcgctttc gctcaaggac agcgatacgg tccgtctgtt
    10861 catttttgaa cgggggggcg ccatcgcctc tgagatcatc atccgctata acgagtacaa
    10921 gaagggtcaa gtgtaattat gtattaccgc tataaatcaa agaaacggga caacaagctg
    10981 gtcaagttcc tgtttatcgc cgtttgcctg tgcgtaataa tcttcctggg ctatacctat
    11041 cgaagccatc tgatgttttg gaagtattcc tacaataagc tctatgcgaa aatcagtcgc
    11101 attcaggcgg tcaaggaccc cgcgctgcgg cggacccagc ttgtcgagct tacgaagcaa
    11161 tgcgatggcg caaaaaccga caatgagatc gatccggaca ccttttttct cgccgggcgc
    11221 gcacactacc agctcgggga ggagctggtg aaccgttcct tcacccgcat ggtgatagac
    11281 gatgcgctga ccgtccatga cgccgcggcg cgacgggagt tcatgcaggc gatcaaggac
    11341 atccgcaaag ggttcgcgct gctcaagaag gacgacgtca cggagaacca tctctacctc
    11401 gcgcgggcga tgctctacct caatttcgca tcgccgaacg aattgtacgc actggtgcag
    11461 cggtacgaca ccgaccgcct taaaggaatg cccgaacagg cgcgggtgct ggccaccctc
    11521 gcggtgatgg cgggcgctgt cgagcccggg cttgcgatca tgcgggggta tggcgcgccg
    11581 gagggcgtcg agggcctgct gtttctcgcc gcgctcgagc ggacgggaaa gcgctacacc
    11641 agcgcgatcg gcgagtacaa gaaggtgttg cagtcgagcc aggacgccgc ggcgctcaag
    11701 aatgcgcacg ccaacctcgg acggatctat tacatccagg cgctgtatcg cgaatcggcc
    11761 gaccacttcg agcacgcgct catgctcgat aaaaacgacg ccgcggtcaa ggtgcaggcg
    11821 ggcaaggcgc acgccgcgct gggaaacaag gcgcgtgcga cggccctctg gaccgaggcg
    11881 ctcgccgcag acgcggcgaa tgaagaggta aaaaaacttc tcggcgcgcg ctgaggccgg
    11941 tatttgtcgg ataatggctt gacaaaaaac ggcggtaaaa tttactaccg ggcaaaacca
    12001 tcgcgcggga caatccacag gagacgataa atgattttcg acaagtttta cagcatgttt
    12061 tcgaacgaca tgggaatcga tttgggcacg gcgaacacgc tcgttcatgt gaaggggcag
    12121 gggatcgtgc tcagcgagcc gtcggtcgtc gcctgtcaaa cgagcacggg aaaggtcctg
    12181 gcggtgggcc atgaagcgaa gcggatgctc ggccggacgc ccggcgacat cgtcgccatc
    12241 cgcccaatga aggacggggt gatcgccgat ttcgaaacga ccgaaaagat gatccggtat
    12301 tttatcacca ag
//
LOCUS       DAORHF010000099        12306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4259_length_12306_cov_6.220962, whole genome shotgun sequence.
ACCESSION   DAORHF010000099 DAORHF010000000
VERSION     DAORHF010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12306
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4259_length_12306_cov_6.220962"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="PKJ16_09695"
     CDS             complement(<1..1116)
                     /locus_tag="PKJ16_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47303.1"
                     /translation="MTRLARFLCALAVVMIAAAAQGGDAPGNDLVAKRDAFIAQHGDL
                     PVGEVSVHGLAKTRASVVFDSVDLAPGAPLSRVDPERLLQQLHAVGLFKRIDIRYAHR
                     GDHAAVIIDLEEKWTLIPIPFVFSSRSVLRYGFFVFDTNLLGYNKKLFVGAMNSRQGW
                     NANAVYIDPMIMGSRFTGTVAGVVGNRIYEDADFDRDVYRRYRADFSSLTGTVGHEFR
                     RTATVSLVGRFQHASVDPSYVDAIDAPSSEQSFATGLRLRYRDQRYHLYTSEGVRAEA
                     EYLHGFSAGSRGAEYDQGKITAEFARFFLTSHLVTLGVKGMRSNLPEVFQERPGGQMG
                     YKTVPSEVVQAHCHYTAYAVYERPIYRPTWGTFTALAF"
     gene            complement(1113..1397)
                     /locus_tag="PKJ16_09700"
     CDS             complement(1113..1397)
                     /locus_tag="PKJ16_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQ-loop repeat-containing protein"
                     /protein_id="HNV47304.1"
                     /translation="MHTADIIGWIGSLAFALCGVPQAWQCVRDRTARGINPLFLALWL
                     TGELCYVASVLLKFGWVGWMMFNYCTNIASVLVIGYFLARDRLRPGAPSS"
     gene            complement(1408..1515)
                     /locus_tag="PKJ16_09705"
                     /pseudo
     CDS             complement(1408..1515)
                     /locus_tag="PKJ16_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011947996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            2755..3645
                     /locus_tag="PKJ16_09710"
     CDS             2755..3645
                     /locus_tag="PKJ16_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47305.1"
                     /translation="MRESTKTLMRKHGRRIDRALHNYLYFRWYYPYVKAASLAVGALK
                     FVSWIAPLKHVGRAVFNRYHSKVLSGEDARRILTLNEDVRVVSDANRRIVPYPYAHSI
                     IFQDPEFVAVMDCPCKKSYRAPADTINSCIVVGSGTGRFWLDTCAKYNARRISQEEAL
                     AIVRRFRGMGYVTQAFFKVATGGSTGVICNCHPDSCVSLKATAEARRLDPSLSMNAES
                     GYAVRRDTSTCVACGACASACHFGAMAMTNGAPEYAMGRCMGCGLCVERCRHGAITLH
                     RDPGKTMPLDLDLVREGIGR"
     gene            complement(3670..4674)
                     /locus_tag="PKJ16_09715"
     CDS             complement(3670..4674)
                     /locus_tag="PKJ16_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV47306.1"
                     /translation="MPYIGLRAPPTRRSEETARTSITRVSVIVLLLSRLFSKLSLPTA
                     LAIGRFLGRLAYYLIPVKRGIARENVRRVFGAELSPREQRRIVRKCYESMCMWGMEVM
                     RMPHLTPEVSEQTVTREGYEYMEQALARGKGAILVASHMDNVDLAGCSMAIRGLPLSV
                     AVKEFKSKSLQDFLSAVRERTGVILIPPRKSKEQIIDLLKKNVVVTLVVDQHMAKHRA
                     IVCEFFGQLAATSPAPARFAFETGAPIIPGLMYRTDNKGGHGIRLGPPMEMETPYDDL
                     DKNIRHNTERINRIIEGWIREFPEQWLWLHKRWKVQDNPEGWDIPEHLQHLVKRPAKG
                     "
     gene            4782..6251
                     /gene="tilS"
                     /locus_tag="PKJ16_09720"
     CDS             4782..6251
                     /gene="tilS"
                     /locus_tag="PKJ16_09720"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HNV47307.1"
                     /translation="MIRHSYLMGADIGDIVFERVRAFIEAHRLIAPGERVLLALSAGK
                     DSMTLFDLFLRLRDEIGFSFNVFHLNHMVRGADADADEAFVRELAQRNGIAFHGARFD
                     FANELSRGESFEEQARRVRYALLDERARANGCARIATAHNRDDQAETVLMRILAGTGV
                     HGLAGMAPARGAIIRPLLDVDAREAYEYLRARGIEWREDESNRDLRYRRNYIRHALLP
                     EIRSRFPDADAALLSLAAVAREYRDMADELAAGRYGAAARREGDATVLPCGAWCGNPA
                     LFRHVIASALRAAGKPYVTAGIIGEIERRFRAPRANQVLYRDARIAMVKRLRDGAAAV
                     IITPQKKEDDCAAAGYSVVIDAARLPARIALPAAGCEVLLRDASYEEFAAAGASSERA
                     YVAIDDAHAELVLRSRRPGDRIALERGSKKIKDLMIENKLDNDAKRVIPLLVVDGHIA
                     AYLPGFTGRGTNRVAAGFRVSPTSARVMALEREGPSRFF"
     gene            6348..8213
                     /gene="ftsH"
                     /locus_tag="PKJ16_09725"
     CDS             6348..8213
                     /gene="ftsH"
                     /locus_tag="PKJ16_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HNV47308.1"
                     /translation="MNKTAKQIALVLLIGVLGIAIFRMGSMPRQNIENLQYSDFYKKV
                     LDKKIKGVTIINGKTIQGTYLKGETPVHFKTLVPYEDPTLLKLLIDNKIEVKGQEEED
                     SYILKGILQFLPWLIFFGLIWFLIMRQIQTTGNKAMSFGKSRAKLNPETKTKITFADV
                     AGVDEAKEELKEIIDFLKDPKKFVNIGAKIPTGVLLMGPPGTGKTLLARAIAGEAGVP
                     FFSISGSDFVEMFVGVGASRVRDLFDQGKKSAPCIIFIDEIDAVGRLRGAGLGGGHDE
                     REQTLNQLLVEMDGFDTNEGVIIVAATNRPDVLDPALLRPGRFDRQVVVDTPDIKGRE
                     KILAVHSKKVPLSREVDLKVIARGTPGFTGADLANLVNEAALLAARRNKKRVSMLEME
                     DAKDKVMMGPERRSMIISDDEKRVIAYHEAGHTILGILAGNDPVHKVTVIPRGRALGL
                     TMQLPREEKHIHPKSYWLNQITMLFGGHVAESIQFQDLSTGSSNDLERATDIARKMVC
                     EWGMSDRIGPLSFGKRNEQIFLGREIAQHRDYSEETARLIDDEVKSVITTCEARARGI
                     IMDNRDKFDAIAKGLIERETLNADEVDAIMRGEALPPMAANGASEEPAPAESPAT"
     gene            8229..9938
                     /locus_tag="PKJ16_09730"
     CDS             8229..9938
                     /locus_tag="PKJ16_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47309.1"
                     /translation="MGTHDDLERLLADVDEKTDYEISRDQSLRDSERRARRADSAVEG
                     GEGDAAGAVTVEEFVARYGEDIEFFRSGMTAVLGEHQRIDAEFKKLFSDSQIPRDVDD
                     IIKKYRYTYLKYLENIEDLVNEHTRYLSPDALLRESKKNFHKKFQGYEDENRLAFLSL
                     KEIFLRLREFNSRVKKEWDELARSIEVINLSSSGRAYYAELSSRCAAAAEFCRRTETF
                     LHFVATVLSLGPDDYYAIEKDIYGKIRYFENFSYQYENLFRRSADAEREVMRSHDDAL
                     QLDDGRYAVGAAEDDEPAPVETEPPRHAQIERTVEPEPAPSPGPPAPPRSSFAPAVAF
                     TVTGTRTWNTREPYVLALRVDALERDYVDLGSSFFYIDSPADEAALKAAIKIAMIKFL
                     RDRSQNIVESYRGFVHHVIRDAIGEAGSFFDLSADRLQLFTYHLGPLTTSRIVTAVFQ
                     ARGIGSCYKYVEGSVVKRFLPAEFIREASLMWHEQYINTLELPFDRIQEFDELRKLVS
                     RTYHAEIQRVLGIIRKVDVESKLAKKTDAEKLEIFRDRWMQWFGAVPMAVYRRFVEKS
                     LLR"
     gene            complement(9930..12026)
                     /gene="recG"
                     /locus_tag="PKJ16_09735"
     CDS             complement(9930..12026)
                     /gene="recG"
                     /locus_tag="PKJ16_09735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HNV47310.1"
                     /translation="MLEQSVQFVKGIGPTKAALLASQAGIETVEDLLYHSPRRYLDRS
                     TFKRIADCRTGETVTVGGRIADAAIMGRGRKFLSVTIEDESGSLAGVFFGGIPYFKKI
                     FRPGEYVLFSGKIDFFRTMQIVHPEFDFLDDESSIANINTGRVVPLYRSSEKLRGAGL
                     DSRGFRRIIRNALDACIGEIADPLDAGLLERLGLISLPEAIRFLHFPDTAQDADRARR
                     RLSFNELFLLQYYLALSRRLLREERGASARAIDTAAFDAFARGLPFALTDAQAAAIDE
                     IRRDMERSVPMNRLLQGDVGSGKTVVSLAAALFALGRGEQTAVMAPTEVLAAQHDRTF
                     RALLPPGVRTALLTGSLPRAEKRAIASAAAAHELDIVIGTHAVIQNGVSFARLGLIVI
                     DEQHRFGVNQRAALRDKGALPDLLVMTATPIPRSLALTLYGDLEVSSLREKPAERVPV
                     TTLAFPEERVADIYRSVEKYVSQGRQVFIVLPLIAESEKMDLASAEDTYARLRDTVFP
                     ARRVGLLHGRLPAAEKDAVMRDFAAGAVDILVTTTVVEVGVDVPNATVMVIMHPERFG
                     LAQLHQLRGRVGRGAHQSFCILVHPADIPDESRRRIDAIVGTDDGFAIAEEDLRQRGA
                     GEVIGLRQHGHDAGLEFTDLARDMELILSARAEAEARAAEVADAETLAARVREPAQIG
                     GIARGIRSKRVISLLS"
     gene            complement(12033..>12306)
                     /locus_tag="PKJ16_09740"
     CDS             complement(12033..>12306)
                     /locus_tag="PKJ16_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47311.1"
                     /translation="FLVLILMIIGLVVYFLYFFNAPRKDAVLPGAGMRGNLTVTVFGN
                     DGKIIKRWTGMKRAAARDAATTNLFTTREGKSVQIGPAVWFIAEEE"
BASE COUNT         2184 a         3922 c         4043 g         2157 t
ORIGIN      
        1 gaaggcgagc gcggtgaagg tcccccacgt tgggcgatat atcgggcgct cgtagaccgc
       61 gtaggcggtg tagtggcagt gcgcctgcac cacctcgctg gggaccgtct tgtaccccat
      121 ctgcccgccg gggcgctcct ggaagacctc cggcaggttc gatcgcatgc ccttgacgcc
      181 cagcgtcacc aggtggctcg tcagaaagaa gcgcgcgaac tcggccgtga tcttcccctg
      241 gtcgtactcg gcgccgcgcg agcccgccga aaagccgtgc agatattccg cctcggcgcg
      301 gaccccctcg ctcgtataga ggtgatagcg ctggtcgcgg taccgtagcc gcagccccgt
      361 ggcgaaactc tgctcggacg acggcgcgtc gatcgcatcg acgtacgacg gatcgaccga
      421 ggcgtgctgg aaacgcccga cgagcgacac ggtggcggtg cgcctgaact cgtggcccac
      481 ggtgccggtg agcgacgaga agtcggcccg gtagcgccgg tacacgtcgc ggtcgaaatc
      541 ggcgtcctcg tagatgcggt ttcccacgac gcccgcgacg gtcccggtga agcgcgatcc
      601 catgatcatc ggatcgatgt acaccgcgtt cgcgttccat ccctgacgcg agttcatcgc
      661 cccgacgaag agcttcttgt tgtacccgag cagattggtg tcgaagacga agaagccgta
      721 gcggagcacc gagcgcgacg agaagacgaa ggggatgggg atgagcgtcc acttctcctc
      781 caggtcgatg atgacggcgg cgtggtcgcc ccggtgcgca taccggatgt cgatgcgctt
      841 gaagagcccc accgcgtgga gctgctgcag cagccgctcc ggatcaacgc gcgaaagcgg
      901 cgcccccggc gcgaggtcca cgctgtcgaa caccaccgac gcgcgcgttt tcgcaagccc
      961 atggaccgat acctcgccca cggggaggtc gccatgctgc gcgatgaagg cgtcgcgctt
     1021 cgcgacaaga tcatttcccg gcgcgtcgcc accctgggcg gcggccgcga tcatcacgac
     1081 cgcaagcgca cacaggaatc gcgcgagacg cgtcatgacg atggggcgcc cggtcgcagc
     1141 cgatcgcgcg ccaggaagta gccgatcacg agcacggagg cgatgttggt gcagtagttg
     1201 aacatcatcc agcccaccca gccgaacttg aggagcacgc tggccacgta gcacagctcc
     1261 cccgtgagcc acaacgccag gaagagcggg ttgatgccgc gcgcggtccg gtcgcgcacg
     1321 cactgccacg cctgcgggac gccgcagagg gcgaaggcga gcgaaccgat ccagccgatg
     1381 atgtcggcgg tatgcattgc ggtggcgtta tctgccgcaa cagtccttgt atttcttgcc
     1441 gctcccgcag ggacagggat cggcgcgttt cacttcgcgc gttgatcggt tttttttctt
     1501 tgccggccgt cgcttcgccg gtcgatcggc ggatttcgtt gcgttacgct ttttccttgt
     1561 tttccctgcg aatgtatcga acgcggacgc gaaatacccg tcaagcacct ccgggtcgtc
     1621 catgtcgtac ccttcggccc gcccctgcat gacgatctgc ttcgccatgc tccagcgcgt
     1681 gctgtcgcgg gagcgctcca cgatggcgtc cgcgattttc aaggctgcgc gtgctataag
     1741 ctttccatgg gagtggatgc cttcgtcctc catgaaggag aaaaaggcgg caaggaccgg
     1801 cgcgatcgcc cgaaaaaacg gctcctcgtt gggaatctcg gcgggaagaa tctccaggca
     1861 gcatgtttcc atcgattgac ggtcgcattc ctcgtgtggg atggtgtgcg ttcgaaacag
     1921 gtgttccgcg aatgtcgcaa ggatggacgg agcaagctcc ctctgctcgt cggacagttt
     1981 cttgaaccgc tgactcgcgg aaaaacggcc atactgctgt tttcctcgat tgtggagctg
     2041 ttcaatttcg tcccgcaggg ggtcgtcgtc gttgtcatcg atattcacgc tctcgtactc
     2101 ttcggcgtaa ttgaggcagg cgataattgt ccatgtattg aataacagga cgggttcgtt
     2161 cttgcggcgg atgaatgatt tcctcgggtt ttcgggatcg ggttctgtta taatgctgat
     2221 ggcgaacgcc ataagattcg gttgaccgga gagatctaag agcgtatcga acatctgttc
     2281 gaagtcggtc tgggatgtgc tcatctcggc gatcggtccc tcgaagcggt ccatgacgca
     2341 atcaaccatc gtctccgtga cctcgggaat ggcggtattg taggagcgca tgatatgcca
     2401 ggtcaacgtg ctggctacga gcaggagcgt gcgttcgttg tcgttcaggc ttttctcgtc
     2461 gaccgcggca aacgcatgga acaggtacgg ctgtttctcc tcgaattcct cgacgatctg
     2521 gtcggtaata acggatgggt catcgtcgat tccatccaat actttttcga tttgcttttt
     2581 tgtgatccgt ttcatcgtgc ctgggccccc gcgtgaattg gacgccattt gacgacgcca
     2641 accggcgaaa tcaaacactt ttccgcgacg gtcgcctgcg aggttccgcg tatgtcttga
     2701 catggataat ccatccggta tgatccgtac cggtgatacg gactgcggac gcccatgaga
     2761 gaatcaacaa aaacgctgat gcgcaaacac ggccggcgga tcgaccgcgc cctgcacaac
     2821 tacctttatt tccgctggta ctatccctac gtgaaggcgg cgagcctggc ggtaggcgcg
     2881 ctcaagtttg tttcgtggat tgcgcccctg aagcatgtcg ggcgcgcggt ctttaaccgc
     2941 taccacagca aggtcctctc cggcgaggac gcgcgcagga tcctcacgct gaacgaggac
     3001 gtccgcgtgg tctccgacgc gaaccggcgc atcgtgccct atccctacgc gcacagcatc
     3061 atcttccagg accccgagtt cgtcgcggtg atggactgtc cgtgcaagaa gtcgtaccgc
     3121 gcgccggctg acacgatcaa ctcgtgcatc gtggtcggga gcggcaccgg gcggttctgg
     3181 ctggatacgt gcgcgaaata caatgcgcgg cgcatctcgc aggaggaggc gctcgcgatc
     3241 gtgcgccggt tccgcgggat gggttacgtg acccaggcct tcttcaaggt ggcgacgggc
     3301 ggcagcacgg gcgttatttg caactgccac ccggattcct gcgtgagcct caaggcgacg
     3361 gcggaagcgc ggcggcttga tccgtccctc tcgatgaatg ccgaatccgg ctatgccgtg
     3421 cggcgtgata catcgacgtg cgtcgcgtgc ggcgcctgcg cgtccgcgtg ccatttcggg
     3481 gctatggcga tgacgaacgg cgcgcccgaa tatgccatgg ggcgttgcat gggctgcggc
     3541 ctctgcgtgg agcgatgtcg gcacggggcg ataacgctcc atcgcgatcc ggggaagacg
     3601 atgccgctgg atctggattt ggtgcgtgag gggatagggc gttaggcgcg agtcaccgta
     3661 ggctctcttt caccccttcg ccggcctttt caccaaatgc tgtagatgct ccggaatgtc
     3721 ccatccctcc ggattgtcct gaaccttcca gcgcttgtgg agccacagcc actgctcggg
     3781 gaactcgcgg atccatccct cgatgatgcg gttgatccgc tcggtgttgt gccggatgtt
     3841 tttgtccagg tcgtcgtagg gcgtttccat ctccatcggc gggcccaggc ggatgccgtg
     3901 gcctcccttg ttgtccgtgc ggtacatgag gccggggatg atcggcgcac ccgtttcgaa
     3961 cgcgaagcgc gcgggcgccg gcgaggtcgc ggcgagctgc ccgaagaact cgcacacgat
     4021 ggcgcgatgt ttggccatgt gctggtccac cacgagcgtg accaccacgt tctttttcag
     4081 gaggtcgatg atctgctcct tcgacttgcg cggggggatg aggatcacgc cggtgcgctc
     4141 ccggaccgcg cttaagaaat cctgcagcga tttcgacttg aactccttca cggcgacgct
     4201 taagggaaga ccgcggatgg ccatgctgca cccggcgagg tccacgttgt ccatgtgcga
     4261 ggcgacgagg atcgcgccct tcccgcgcgc cagcgcctgc tccatatatt catagccctc
     4321 gcgggtgacc gtctgctcgc tcacctcggg cgtgaggtgc ggcatgcgca tcacctccat
     4381 gccccacatg cacatcgact cgtagcactt gcgtacgatg cgtcgctgtt cgcgcggcga
     4441 gagctcggcg ccgaagacgc gccgcacgtt ctcgcgcgcg atgccgcgct tcaccggaat
     4501 gagatagtag gccaggcggc ccaggaaacg cccgatggcg agcgccgtcg ggagcgacag
     4561 tttggaaaac aatcggctga gtaggagtac gatgacgcta accctcgtaa tggatgtgcg
     4621 cgccgtttcc tcgctgcggc gagtgggtgg cgcgcgaagc ccgatatacg gcactccgcc
     4681 gcgatttcgt caaattaaaa aacgaaattt cgataaaacc gtcgtcatcg cgatgaacgg
     4741 cgtacggtgc ggcgaacggt ttttttcttt tcattttccg catgatccgc cactcctacc
     4801 tcatgggtgc tgatatcggc gacatcgtgt tcgagcgcgt ccgcgccttc atcgaggcgc
     4861 atcgcctcat tgcgcccggc gagcgcgtgc ttctcgcgct ctcggcgggg aaggactcca
     4921 tgacgctgtt cgatctgttc ctccggctgc gcgatgagat cgggttttcg ttcaacgtgt
     4981 tccatctcaa ccacatggta cgcggggccg acgccgacgc ggacgaggcg ttcgtgcgcg
     5041 aactcgcgca gcgcaatggc atcgcgtttc acggcgcgcg gttcgacttc gcgaacgagc
     5101 tctcgcgcgg cgagtccttc gaggagcagg cgcgccgggt gcgctatgcg ctgctggacg
     5161 agcgcgcccg cgcgaacggc tgcgcgcgga tcgcgaccgc gcacaaccgc gacgaccagg
     5221 cagagaccgt cctcatgcgc atcctcgccg ggacgggcgt gcacgggctg gcggggatgg
     5281 cgcccgcgcg gggcgcgatc atccggccgc tgctggacgt ggacgcgcgc gaggcgtacg
     5341 agtacctccg ggcgcgcggt atcgaatggc gcgaggacga aagcaatcgg gacctgcggt
     5401 atcggcgcaa ctatatccgc catgcgctcc tcccggagat ccgttcgcgc ttcccggacg
     5461 cggacgcggc gctcctgtcg cttgcggccg tcgcgcgcga gtaccgcgac atggccgacg
     5521 agctcgccgc ggggcgttac ggcgccgccg ctcggcggga gggcgacgcg acggtccttc
     5581 cctgcggggc gtggtgcggg aaccccgcgc tcttccggca cgtgatcgcc tccgcgctgc
     5641 gcgcggccgg gaagccctat gtcacggcgg gcatcatcgg cgagatcgag cgtcgtttcc
     5701 gcgcgccgcg ggccaaccag gtcctctacc gggacgcgcg tatcgcgatg gtgaagcgcc
     5761 tgcgcgatgg ggccgcggcc gtcatcatca ccccacaaaa aaaagaggac gattgtgcgg
     5821 cggcgggcta ttccgttgtc attgatgccg cgcgtcttcc cgcgcggatc gcgctccccg
     5881 cggccggctg cgaggtcctg cttcgggatg cctcgtacga ggaattcgcg gccgcaggcg
     5941 cgtcgagcga gcgggcatac gtcgcgatcg acgatgcgca cgccgagctt gtactgcgca
     6001 gccggcgtcc cggcgaccgc atcgcgctgg agcgtggatc gaaaaagatt aaggatctga
     6061 tgatcgaaaa taaacttgac aatgacgcca aacgcgtcat accgcttctc gtcgtcgacg
     6121 gccatatcgc cgcgtatctt cccggtttta cgggtcgcgg gacgaaccgc gtggctgccg
     6181 ggttccgcgt ttcgccgacg agcgcccgcg tcatggcgct ggaacgggag ggtccgtccc
     6241 gttttttttg attgcgtccc gcgtgggcgt tatactattg ccacgggccc accggattcc
     6301 gccgagcgcc aggcccgccc cgtaccacat gtatcaagga gctgtagatg aataagaccg
     6361 cgaaacagat cgccctggtc cttttgatcg gcgtgctggg gatcgccatc tttcgtatgg
     6421 ggagcatgcc ccggcaaaac atcgagaacc tgcaatacag cgatttctac aagaaggtgc
     6481 tcgataaaaa gatcaagggc gtgaccatca taaacggcaa gaccatccag gggacctacc
     6541 tcaagggcga aacgccggtg catttcaaga cgctggtgcc ctacgaggac cccacgctcc
     6601 tgaagctgct catcgacaat aaaatcgagg tgaagggcca ggaggaggag gacagctata
     6661 tcctcaaggg gatcctccag tttctgccgt ggctcatctt cttcgggctc atctggttcc
     6721 ttatcatgcg gcagatccag acgaccggca acaaggcgat gtccttcggc aaaagccgcg
     6781 ccaagctcaa tcccgagacc aaaacgaaga tcaccttcgc cgacgtggcg ggcgtggacg
     6841 aggcgaagga ggagctcaag gagatcatcg acttcctcaa ggacccgaag aaattcgtga
     6901 acatcggcgc gaagatcccc acgggcgtcc tgctgatggg ccctcccggc acggggaaaa
     6961 cgctcctcgc gcgcgcgatc gcgggcgagg cgggcgtgcc gtttttctcc atcagcggct
     7021 ccgatttcgt ggagatgttc gtgggcgtgg gcgcctcgcg cgtgcgcgac ctgttcgacc
     7081 agggcaagaa gagcgcgccg tgcatcatct tcatcgacga gatcgacgcc gtggggcgcc
     7141 tgcgcggcgc gggcctgggc ggcgggcacg acgagcgcga gcagacgctc aatcagctgc
     7201 tggtggagat ggacggcttc gacaccaacg agggcgtgat catcgtggcc gcgaccaacc
     7261 ggcccgatgt gctggacccc gcgctcctgc ggcccggccg cttcgaccgg caggtggtgg
     7321 tggacacgcc ggacatcaag gggcgcgaga agatcctggc cgtgcactcc aagaaggtgc
     7381 cgctctcgcg cgaggtggac cttaaggtga tcgcgcgcgg aacgcccggc ttcaccggcg
     7441 ccgacctcgc caacctggtg aacgaggccg cgctcctggc ggcgcggcgc aacaagaagc
     7501 gcgtctccat gctcgagatg gaggacgcga aggacaaggt catgatgggc cccgagcgcc
     7561 gctccatgat catatccgac gacgaaaagc gcgtgatcgc ctaccacgag gccgggcaca
     7621 ccatcctggg gatccttgcc ggcaatgacc cggtccacaa ggtcacggtg atcccgcgcg
     7681 gccgcgcgct gggcctcacc atgcagcttc cccgggagga gaagcacatc catcccaaga
     7741 gctactggct caaccagatc acgatgctct tcggcggcca cgtggccgaa tcgatccagt
     7801 tccaggacct ctccaccggc tcgagcaacg acctcgagcg cgccaccgac atcgcgcgca
     7861 agatggtctg tgaatggggc atgagcgacc ggatcgggcc gctctccttc gggaagcgca
     7921 acgagcagat tttcctgggg cgcgagatcg cgcagcaccg cgactacagc gaggagaccg
     7981 cgcgtctcat cgacgacgag gtgaagtcgg tcatcacgac ctgcgaggcg cgggcgcgcg
     8041 gcatcatcat ggacaaccgc gacaagttcg acgcgatcgc gaagggcctc atcgagcggg
     8101 agacgctcaa cgccgacgag gtggacgcca tcatgcgcgg cgaggcgctc ccgccgatgg
     8161 cggccaacgg cgcgtccgag gagcccgccc ccgcggagag ccccgcgacc taggatcggg
     8221 accggcccat gggaacgcac gacgacctcg aacggctcct cgccgatgtc gatgaaaaga
     8281 ccgactacga gatcagccga gaccagtcgc tccgggacag cgagcgccgg gcgcgccgcg
     8341 ccgacagcgc cgtcgagggc ggcgagggcg acgccgccgg cgcggtgacg gtcgaggagt
     8401 tcgtcgcccg ctacggcgag gacatcgagt ttttcagaag cggcatgacg gccgtcctgg
     8461 gcgagcacca gcggatcgac gccgagttca agaagctctt cagcgacagc cagatccccc
     8521 gcgatgtcga cgacatcatc aagaaatacc gctataccta cctgaagtac ctcgagaaca
     8581 tcgaggatct cgtcaacgag catacgcgct acctgtcgcc ggacgcgctc ctgcgcgagt
     8641 caaagaaaaa tttccacaag aaattccagg gatacgagga cgagaaccgc ctcgcctttc
     8701 tctcgctcaa agagattttc ctgcgccttc gggagttcaa cagccgcgtc aagaaggagt
     8761 gggacgagct ggccaggagc atcgaggtga tcaacctgtc ctcgtcgggc cgcgcgtatt
     8821 acgcggagct gtcgtcccgg tgcgccgccg ccgccgagtt ctgccggcgc accgagacct
     8881 tcctccactt cgtggccacg gtcctgtcgc ttggcccgga cgactactac gccatcgaaa
     8941 aggacattta cggaaagatc cgctacttcg agaatttctc gtaccaatac gagaacctgt
     9001 tccgccgatc ggccgatgcg gagcgcgagg tcatgcggag ccacgatgac gcgctccagc
     9061 tcgacgatgg acgatacgcc gtgggcgccg ccgaagacga cgagccggcg cccgtcgaaa
     9121 cggagccgcc ccgtcacgcg cagattgagc ggacggtcga gccggaaccg gcgccgtccc
     9181 ccggtccgcc ggcgccgccg cgctccagct tcgcgccggc cgtggccttc accgtgacgg
     9241 gcacgcggac ctggaacacg cgggagccct atgtgctcgc gctgcgggtg gacgcgctcg
     9301 agcgcgacta tgtcgatctg ggatcgtcgt tcttttacat cgactcgccc gcggacgagg
     9361 ccgcgctcaa ggccgccatc aagatcgcga tgatcaagtt cctgcgcgac cgctcgcaga
     9421 acatcgtcga gtcgtatcgc gggttcgtgc accacgttat ccgcgatgcg atcggcgagg
     9481 cggggtcgtt cttcgacctc tccgccgacc gattgcagct ctttacctat catctggggc
     9541 ccctcaccac gagccggatc gtgacggcgg tcttccaggc gcgcggcatc ggaagctgct
     9601 acaagtacgt cgaggggtcg gtggtgaagc gcttcctgcc ggcggagttc atccgcgagg
     9661 cgtcgctcat gtggcacgag cagtacatca acacgctgga gcttcccttc gaccggatcc
     9721 aggagttcga tgagttgcgc aagctcgtat cgcgcacgta tcacgccgag atccagcgcg
     9781 tgctgggcat catccggaag gtcgacgtgg agtcgaagct cgcgaaaaag accgacgccg
     9841 agaagctcga gatcttccgc gaccgctgga tgcagtggtt cggcgccgtc cccatggcgg
     9901 tctaccgccg gttcgtggaa aaatccctct tacgataaca gcgagatcac gcgcttgctg
     9961 cggatgccgc gagcgatccc gccaatctgc gccggctcgc gcacgcgcgc cgcgagcgtc
    10021 tccgcgtcgg cgacctccgc cgcgcgcgcc tcggcctccg cgcgcgcgga gaggatgagc
    10081 tccatgtcgc gcgcgaggtc cgtgaactcg aggcccgcgt cgtggccgtg ctgacgcagg
    10141 ccgatcacct cgccggcgcc gcgctggcgg aggtcctcct cggcgatcgc gaagccgtcg
    10201 tcggtgccca cgatggcgtc gatgcggcgg cggctctcgt ccgggatgtc ggccggatgc
    10261 accaggatgc agaaggactg gtgcgcgccc cgtcccacgc ggccgcgcag ctgatggagc
    10321 tgggccagcc cgaaccgctc cgggtgcatg atcaccatca ccgtggcgtt ggggacgtct
    10381 acgcccacct cgaccaccgt cgtggtcacc agaatgtcga cggcgccggc cgcgaagtcg
    10441 cgcataacgg cgtccttctc ggcggccggg aggcgtccgt gcaggagccc gacgcggcgc
    10501 gcggggaaca ccgtgtcgcg cagccgggcg taggtatcct ccgccgaggc gaggtccatc
    10561 ttctccgact cggcgatgag cggcaggacg atgaagacct gccggccctg cgagacgtac
    10621 ttctccaccg agcggtagat gtcggccacg cgctcctcgg ggaaggcgag ggtggtcacg
    10681 ggaacgcgct cggcgggctt ctcgcgcagc gacgagacct ccaggtcccc gtagagggtg
    10741 agggcgagcg agcgggggat cggcgtcgcc gtcatcacca gcaggtcggg aagcgcgccc
    10801 ttgtcgcgca gcgccgcgcg ttggttcacg ccgaagcggt gctgctcgtc gatgacgatg
    10861 aggccgagcc gagcgaagga gacgccattc tggatcacgg cgtgcgtgcc gatgacgatg
    10921 tcgagttcgt gcgcggcggc agcggaggcg atggcgcgct tttcggcgcg cggaaggctc
    10981 cccgtgagga gggccgtgcg cacgcccggg ggaaggagcg cgcggaaggt gcgatcgtgc
    11041 tgcgcggcca gcacctcggt gggcgccatc acggccgtct gctcgccgcg tcccagcgcg
    11101 aaaagcgcgg cggcgagcga gaccaccgtt ttccccgatc ccacgtcgcc ctgcaagagc
    11161 cggttcatgg ggacggaccg ctccatgtcg cgcctgatct cgtcgatggc ggcggcctgc
    11221 gcgtcggtga gcgcaaacgg aaggccgcgc gcgaacgcgt cgaaggcggc ggtgtcgatg
    11281 gcgcgcgcgg acgcgccgcg ctcctcgcgg agcagccgtc gtgaaagcgc gagatagtac
    11341 tgcagcagga agagctcgtt gaacgagagg cgccgccggg cgcgatcggc gtcctgggcc
    11401 gtgtcgggga agtgcagaaa gcggatcgcc tcgggcagcg agatgagccc cagccgctcg
    11461 aggaggcccg cgtccagcgg atcggcgatc tccccgatgc aggcgtcgag cgcgtttcga
    11521 atgatgcggc ggaacccgcg cgagtcgagc ccggccccgc gcagcttctc gctcgagcgg
    11581 tatagcggca ccacgcgccc ggtgttgatg ttcgcgatcg agctctcgtc gtcgaggaaa
    11641 tcgaactccg ggtggacgat ctgcatggtc cggaagaaat cgatttttcc ggagaagagg
    11701 acgtactcgc cggggcggaa tatctttttg aaatacggga tgccgccgaa gaagacgccg
    11761 gcgaggctgc cggactcgtc ctcgatggtc accgagagaa atttccggcc gcggcccatg
    11821 atcgccgcgt cggcgatgcg gccgcccacc gtcacggttt cccccgtccg gcagtcggcg
    11881 atgcgcttaa atgtcgatcg atcgaggtag cggcgcggcg agtgatacag caggtcttcg
    11941 acggtctcga tccccgcctg ggaggcgagc aacgccgcct tcgtgggtcc gattcccttg
    12001 acgaactgga cgctctgctc cagcacgcac ccttactcct cttctgcgat aaaccacacc
    12061 gcgggcccga tctgcaccga ctttccctcg cgggtcgtaa agaggttcgt cgtggcggcg
    12121 tcccgcgcgg cggcgcgctt catgccggtc cagcgcttga tgatcttgcc gtcattgccg
    12181 aagaccgtca ccgtgaggtt cccgcgcatg cccgctcccg ggagcacggc gtctttccgc
    12241 ggcgcgttga aaaaatacaa gaaatagacc accagcccga tgatcataag gattaagacg
    12301 aggaac
//
LOCUS       DAORHF010000100        12140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4367_length_12140_cov_7.134299, whole genome shotgun sequence.
ACCESSION   DAORHF010000100 DAORHF010000000
VERSION     DAORHF010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12140
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4367_length_12140_cov_7.134299"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PKJ16_09745"
     CDS             <1..1864
                     /locus_tag="PKJ16_09745"
                     /inference="COORDINATES: protein motif:HMM:NF020660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1926 domain-containing protein"
                     /protein_id="HNV47312.1"
                     /translation="QLEIVGGGFYEPIFSIITESDIRGQLSMMQDYCRTKLGATPRGF
                     WTAERVWDPEIPRLVRGFGLEYTILDNNHFQYAGLEEDDLYGYYITERLDHRLCVFPI
                     DKLLRYYLPYHEPDDTVSYFRTKTEKLGEALFVYGDDGEKFGMWPGTYDWVFERKWLV
                     NFIETILKHDWIITTHPSAYIERIAPKGRIYITQGSYFELSEWSLPPAAATRFNRLNR
                     EVRSWGREHEFFPFMKGGVWNNFLTKYPESNAINKRTVLLSREIDALAQAGDDSLGPI
                     TRELYRAECNCAYWHGLFGGIYLGMLRDALHHHLLLAEGMLVAARGGTTIEIIERDIW
                     NEGSDQILVRTPLQSTVIAPHHGGGVAELGLYAGGINLFNVVARRPETYHDILRELNE
                     DDGGADEVRSIHDQKPVAKEKGLKELLVYDPGRRYSFRDSFFESPPAAEDLMRRRADC
                     VECGDLPYNAEASTTAGAASVTLSRYQVSGGRALSVEKTLTIARDAAGIVARYAITGA
                     AGLIAAVTVDFNLLCDTDEYRGYEIPGVAPEDARLCSIGQSGPLTSCAAFDETRSLRI
                     EFTSSRPATLLRHPIYTVSQSESGFEKNYQGSSLVFLFPVEADRFECTIELRVC"
     gene            complement(1985..2347)
                     /locus_tag="PKJ16_09750"
     CDS             complement(1985..2347)
                     /locus_tag="PKJ16_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47313.1"
                     /translation="MAPDTEHFDQDTDRALVESEVVALFGSENIVHAIKTYRADTKQK
                     EISLEFVKSYLQFINEIAAHDAVSNRDRSDPEVRDLVSHVNLMLRKRDEDVFASMVLH
                     CNRHYRQIIKGGARKAAE"
     gene            2548..4542
                     /gene="tkt"
                     /locus_tag="PKJ16_09755"
     CDS             2548..4542
                     /gene="tkt"
                     /locus_tag="PKJ16_09755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HNV47314.1"
                     /translation="MELTAAEYSLIASTIRTLAMDAVQRANSGHPGMPMGCAEIAAVL
                     WGSIINHDPADPRWVNRDRFVLSAGHGSMLLYAALHLAGYDITLDDIRQFRQLDSKTP
                     GHPEYRHTPGVETTTGPLGQGFANAVGMALAARLLANEFNAAADPIIDHRVYALVSDG
                     DLMEGISAEAASLAGHWGLGNIIFIYDDNGISIEGSTALAFSDDVRRRFEACHWHVQS
                     VDGHDCVQIARAVRAAQAETGRPSLIIAKTAIAKGSPGKEGSEDSHGAPLGADEVKKT
                     KINIGCDPDAEFCVPQAAYDIFARRREELATARRAWDERFSRALRPDERAKWDAFFSR
                     PNIDALRAKLPAFKIGDSIATRSASGSVIESLFAALPNIVGGSADLAPSNKSFAKGHS
                     ETGRNAVGRNIHFGIREHAMGAIQNGIAYYGGFIPYSATFFVFMDYMRPPVRIAALAG
                     LQAVYVFTHDSLFVGEDGPTHQPVEHLAAARAIPNLTTIRPADAEETREAWLAAIANT
                     AGPTALALSRQNLPVLPRITPTGAADLHRGAYVIWEAGPDASVLMLASGSEVQLAIEA
                     AEALRERSVSCRVISFPSWELFDRQDDAYRRAIIPTEQKNRVVIEAGRRMGWERYAGD
                     SALYITMEGFGVSAPQNDLAKRFGLTRDSVVERVLGYLAR"
     gene            complement(4535..5251)
                     /locus_tag="PKJ16_09760"
     CDS             complement(4535..5251)
                     /locus_tag="PKJ16_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV47315.1"
                     /translation="MFVIALVIKAVTAPFDRRLRALHMFTCFWGSFYTWIMPPWRITI
                     EGREKIRRGATYMVVSNHQSQLDILVSFRLFFHFKWVSKVEMFKVPLIGWNMALNRYI
                     KLTRGVKESVQEMMQACEERLREGSSVFMFPEGTRSFDGTVKAFKSGAFILAKKLKIP
                     ILPIVISGTMKALPKYSVNFHGIQKMCIRVLDEVPYHAFENLSVEETAELVRGIIVTN
                     LARLNEQLEGAHQEAAAQLT"
     gene            complement(5523..6293)
                     /locus_tag="PKJ16_09765"
     CDS             complement(5523..6293)
                     /locus_tag="PKJ16_09765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV47316.1"
                     /translation="MTISITIIGHNEAGHLEELLPSLSWADEIVYVDCESADRSLAVA
                     KRHGCRTFSRPNNPNLNVNKSFAMDKATGDWILYLDPDERIPEALRDEVLGVVGSPSG
                     AVAFELGRRNHYFGRWLRHGSKYPDTQLRLFKRGAARFPNRHVHEKLEIQGGTGRLRG
                     DMHHHPFLTVSQFLQKFDFYATFEANFIYANGFRPGPKNAIAYCCIKPLGRFVRRYFF
                     KLGFLDGVEGLFAALFDGVKFIVVYAKIRERARGEAAR"
     gene            complement(6290..7411)
                     /locus_tag="PKJ16_09770"
     CDS             complement(6290..7411)
                     /locus_tag="PKJ16_09770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV47317.1"
                     /translation="MSADGARSVLVMASVRWYNASAHYALFLARALAAAGHHAVVLGL
                     PGSPIVERARAADIPVVEDVNLAASAPWALARNVAGFRRALRGHGIDIIHAHFSRDHT
                     FAAVARAGMGVPIVRTRSETNPPKTHLLNRLQYRHTAEAYTVPGAHLVPVIASLGVPV
                     DRITSIPLAIDRRAFVAQRTNVDVRRALGIPRKRLVVTHLGRLHAVKGVEHFVRSYQY
                     LKNPGRFHFLITGEEIDVRIEDLRRIALDIGASQVTIIGREYDARDILAATDIGAIPS
                     LGSEAICRVALEMFACGIPVVGTAIGSIPETIGAYGGRVVPPADPRAMAEAIEDIAEP
                     KAFRAARASIEAGRARHTEEKFLASNLAVYSSIGREGMS"
     gene            complement(7408..8247)
                     /locus_tag="PKJ16_09775"
     CDS             complement(7408..8247)
                     /locus_tag="PKJ16_09775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV47318.1"
                     /translation="MIKRLMTTREKIESDVLSDIDDDDILGFVLESLKLETDKPYSTL
                     LQVILHYEIAEDNAQRVWFDIVENRRHMAACLNRPVTIKTALVDYFTRTGRSEDIVIF
                     VKENLMRALNDAMMDGLTGLYSHAIIHYELEKEFLLSQRYGFDVCVLFIDIDNFKRYN
                     DEHGHKNGDRLLITVSDQLVETVRKTDKIGRYGGEEFLVVLPHTKLAAGVSLANKIVR
                     MIAKKTATSNRLPEGVTVSIGVAGLSPSIMDGHDLIKAADMAMYRAKRAGKNRVAVAD
                     GIE"
     gene            8370..9122
                     /locus_tag="PKJ16_09780"
     CDS             8370..9122
                     /locus_tag="PKJ16_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47319.1"
                     /translation="MANGNGAPTGLIDFYLDLEDDPEYTGKTGEIFRKILTDYFFGFE
                     TDANKSFALMLGNFEAPDFIAAADSLFNLDLDAFADWINGETLNTSLGGRIMLNPKYL
                     KYAYPHHPPEYNKLPPNVQVELFDRIKKKNREFIDAFHGMMADRAADRGRTILALVAM
                     ILREVHKKTEMPLNRLTQKADAVIRTSFEYCDEIFRASQKQMTELGDDSKVRACIKTF
                     FPVKRAQEIAEIGGVFKQELERMKRRTLRAQQ"
     gene            complement(9143..11329)
                     /locus_tag="PKJ16_09785"
     CDS             complement(9143..11329)
                     /locus_tag="PKJ16_09785"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HNV47320.1"
                     /translation="MTNQFLYGLAATGFCSLAGAGAFLYWLVRLPVSEPAARMLADTR
                     PAQIEFLAHAGRATIVAGLVGTAVMSALVAAGWAHWLIPIACAVGWMLSALCGLVGVL
                     SGMLAAPRVPFALRRSLAHARRVAALPNFIVALCVIGFVLLELFAWLVFVSRALEGEC
                     AAAGGECAGRVAWLAQAPGGAAFRNAAIARWRASEVMALLLACGLGSVAQACFARLTM
                     GRFADAVRVGAGHVRKKIKGLQDGDARNPVLAAETIGAGIAGVAIPCMGAYAVLSSAV
                     RVTLSLATAFPLIAATIGRHAASRPVPDDMAAPIIMGCGVVLVPFLSVACLGISLSPE
                     GPLRRWAGTGLDFLGGLVVIACGGILAWRGLVPAVSFACFAMGIGAMSIVLCAIDIAQ
                     KRTADAVQVHPFNGGNIAWVHVRYVPALMIGILLVVCYASAGGGREALGGWGGIAFGA
                     LGMAVGSFAALPRIARGAVPLWIVSQKGEPYTAEARRGLMMLSGSADRGQYAVRCALG
                     CAAVVVAIALIGASLEQARFWLSRMAGASSLKAGAVALHAVFPDGVVLAPDDLLIALA
                     GFRDLARAFDLSPFNPLLIAGVVLGAAGSYAIAEMLERMTRSGSTAIVEEVRRQFREQ
                     PGIARRSATPDYRTVVQRARAGVSGGDAMRLSAVVLIMGAWCVLAGTAGALGAVMGCA
                     AAGAVPRRADEGWGLMALCAGMFAASSAGIALAFSQASGRIIHMLR"
     gene            complement(11423..>12140)
                     /locus_tag="PKJ16_09790"
     CDS             complement(11423..>12140)
                     /locus_tag="PKJ16_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV47321.1"
                     /translation="RNQKVATALVKVLQKAGYKVGILGPEEGCCGDSALRAGNEYLFH
                     ALATQNLETFKNYGIKKIITTCPHGYNVLKKEYPKFAKVGLGSDGNPLECAYEVYHHT
                     EVIMDIIKKGQIKLKDALAEKVTYHDSCFLGRYNEIYDQPRGIIKAIPGTEFVEMSRN
                     HGQSFCCGAGGARMWIEEHIGTRINQFRTKDAQASGATKIGTACPFCLTMLSDGANEL
                     DIQNLQSFDLAELVYNSMEK"
BASE COUNT         2368 a         4021 c         3671 g         2080 t
ORIGIN      
        1 ccagctcgag atcgtgggcg gcggcttcta cgagccgatc ttttccatca tcacggaatc
       61 ggacattcgc gggcagcttt cgatgatgca ggactactgc cggacgaagc tcggcgcgac
      121 gccgcgcggc ttctggaccg ccgagcgcgt gtgggacccg gagatcccgc gcctggtgcg
      181 cggcttcggg ctggagtaca ccatcctcga caacaaccac ttccagtacg ccggcctcga
      241 ggaggacgac ctgtacggct actacatcac ggagcgcctg gaccatcggc tctgcgtgtt
      301 tcccatcgac aagctgctgc gctactacct gccgtatcat gagcccgacg acacggtgag
      361 ctatttccgc accaagacgg agaagctggg cgaggcgctg ttcgtctacg gagacgacgg
      421 cgaaaagttc ggcatgtggc cgggcaccta tgactgggtc ttcgagcgca aatggctcgt
      481 caacttcatc gaaaccatcc tcaagcacga ttggatcatc accacgcacc catcggccta
      541 tatcgagcgc atcgccccga aggggcgcat ctacatcacc caggggagct acttcgagct
      601 ctcggagtgg tcgctgcccc cggccgccgc cacgcgcttc aaccgcctca accgcgaggt
      661 gcggtcatgg gggcgcgagc acgagttctt tccgtttatg aagggcggcg tgtggaacaa
      721 tttcctcacc aagtatccgg agtcgaacgc catcaacaag cgaaccgtgc tcctgagccg
      781 cgagatcgac gcgctcgccc aggcgggaga cgattcgctc gggccgatca cacgggagct
      841 ctatcgcgcc gaatgcaatt gcgcctactg gcacggcctc ttcggcggga tttatctggg
      901 catgctgcgc gatgcgctgc accatcacct gctgctcgcg gaggggatgc tcgtggcggc
      961 gcgcggcgga acaacgatcg agatcatcga gcgcgacatc tggaacgagg gaagcgacca
     1021 gatcctcgtg cgcacgccgc tgcaatcgac cgtgatcgcc ccgcatcacg gcgggggcgt
     1081 ggccgaactc gggctgtacg ccggcggcat caacctcttc aacgtggtcg cccgccgccc
     1141 cgagacctat cacgatatcc tgcgggagct caacgaggac gacggcggcg ccgacgaggt
     1201 ccgctccatc cacgaccaga agccggtggc caaggagaag ggactcaagg agctgctggt
     1261 gtacgatccc ggccgccgct actccttccg ggactcgttt ttcgagtcgc cgccggccgc
     1321 ggaggacctc atgcgccggc gcgcggactg cgtcgagtgc ggcgatcttc cgtataacgc
     1381 cgaggcgtca acaaccgccg gcgcagcgtc cgtaacgctc tcgcgctacc aggtgagcgg
     1441 cggccgggcc ctctccgtcg agaagaccct cacgatcgcc cgggacgccg ccgggatcgt
     1501 cgcgcgctac gccatcaccg gggcggcggg cctcatcgcg gccgtgaccg tcgacttcaa
     1561 cctgctgtgc gacaccgacg agtaccgcgg ctacgagatc cccggcgtcg cgccggagga
     1621 cgcgcggctc tgctcgatcg ggcagagcgg cccgctgaca tcgtgcgccg ccttcgacga
     1681 gacccgttcg ctgcgtatcg agttcacgtc atcccgtccg gcgacgctcc tgcgacaccc
     1741 catctacacg gtgtcgcagt cggaatccgg gttcgagaag aactatcagg gaagctcgct
     1801 ggtatttctg tttccggtgg aggccgatcg gttcgagtgt acgatcgagc tgcgggtgtg
     1861 ctgatccgcc gctccggcgg ctcacagctt tttcacaatc gccacggcga tgtcgtcggt
     1921 atagaaaaac gcatccagcg acgcgatgat cccgtccccg acctcctgct gcggtcgcct
     1981 gcgcttactc cgccgccttt cgcgccccgc cctttattat ctggcgataa tgacggttgc
     2041 aatggagcac catggacgca aagacgtcct catcgcgttt tctgagcatg aggtttacgt
     2101 gcgagaccag gtcgcgcacc tcggggtcgc tccggtcgcg attgctgacc gcgtcgtgcg
     2161 cggcgatctc gttaataaac tgaaggtagg acttcacgaa ctcgagggaa atttccttct
     2221 gtttcgtgtc ggcgcggtag gtcttgatcg catgaacgat gttctcgctg ccgaacaggg
     2281 ctacgacctc gctctcaacg agggcgcggt cggtatcctg atcgaagtgt tcggtgtcgg
     2341 gtgccatggc gaagttccgt tcccggcgcg tagaaattac agtcgcgtca tcgacgatga
     2401 gcatgcctcc gcaccgcttt cccgtcaacc caaatattcg caaacgcgtt ttccgtttca
     2461 tgaaaaaaat tctctggaca aacggagctc catcgcctcc atgttgcggt gtcgtcattc
     2521 atcatcccat tcactcgagg aaccccaatg gaactgaccg cggcagaata ttctctcatc
     2581 gcctcaacca tccgcacgct cgcgatggac gccgtccaac gagcgaattc cgggcatccc
     2641 ggcatgccca tgggctgcgc cgagatcgcg gcggtgctgt ggggatccat catcaaccac
     2701 gaccctgccg atccgcgctg ggtcaaccgc gaccgattcg tgctctcggc cgggcacggc
     2761 tccatgctgc tgtacgcggc gctccacctc gcgggctacg acatcacgct cgacgacatc
     2821 cgccagttcc gccagctcga cagtaaaacg cccgggcacc cggaatatcg ccacacgccc
     2881 ggcgtggaaa cgaccaccgg gccgcttgga cagggattcg caaacgccgt ggggatggcg
     2941 ctcgccgcgc ggctccttgc gaacgagttc aacgcagccg cggatcccat catcgaccac
     3001 cgggtctacg cgctggtgag cgacggcgac ctcatggagg ggatctccgc cgaggcggcg
     3061 tcgctggctg gccactgggg cctgggcaac atcatcttca tttacgacga caacgggatc
     3121 tccatcgagg gaagcaccgc gctcgcgttc tcggacgacg tccgacggcg cttcgaggca
     3181 tgccattggc acgtgcagtc cgtcgacggc cacgactgcg tgcagatcgc gcgcgccgtg
     3241 cgcgcggccc aggccgagac cggacgcccg tcgctcatca ttgcaaaaac ggccatcgcg
     3301 aagggcagcc ccggcaagga gggaagcgag gattcgcacg gcgcgccgct gggcgccgac
     3361 gaggtgaaga aaacaaagat caacatcggc tgcgatcccg acgccgagtt ctgcgtgccg
     3421 caggccgcct atgacatatt cgcgcgccgc cgcgaggagc tcgccaccgc tcgtcgcgcg
     3481 tgggacgagc gcttttcgcg tgcgctgcgg cccgacgaac gcgcgaaatg ggacgcattt
     3541 ttctccaggc cgaacatcga cgcccttcgc gcgaagctgc ccgcgttcaa gatcggcgat
     3601 tcgatagcga cgcgcagcgc aagcgggagc gtgatcgagt cgctctttgc cgcgctgccg
     3661 aacatcgtgg gcggctcggc cgatctagcg cccagcaaca agtcgttcgc gaagggacac
     3721 tccgaaaccg ggcgcaatgc cgttggccga aacatccact tcggcatccg agagcacgcg
     3781 atgggggcca tccagaacgg gatcgcctat tacggcggct tcatccccta cagcgcgacc
     3841 ttcttcgtgt tcatggacta catgcggccg cccgtgcgca tcgcggcttt ggcgggcctg
     3901 caggccgtct acgtcttcac gcacgactcg ctgttcgtgg gcgaggacgg tcccacgcac
     3961 cagcctgtgg agcacctcgc cgcggcgcgc gcgatcccga acctcaccac catccgtccg
     4021 gccgacgccg aggagacgcg cgaggcgtgg ctcgccgcca tcgccaacac cgcgggaccg
     4081 accgcgctcg cgctctcgcg gcaaaacctg cccgtcctcc cgcggatcac ccctaccggc
     4141 gcggcggacc tgcatcgcgg cgcctatgtg atatgggagg cggggccgga tgcgtccgtg
     4201 ctcatgctgg cctccgggtc ggaggtgcag ctcgccatcg aggcagccga ggcgctccgc
     4261 gagcgatcgg tctcctgccg cgtcatctcc ttccccagct gggagctctt cgaccgccag
     4321 gatgatgcgt atcgccgcgc catcatcccc acggaacaaa aaaaccgcgt tgtcattgag
     4381 gccggtcggc gaatgggatg ggagcgttat gcgggagact cggccctgta tatcacgatg
     4441 gaaggattcg gcgtgtcggc gccccagaac gatcttgcga agcggttcgg cttgaccaga
     4501 gacagcgtcg ttgagcgcgt tctggggtat ctcgctaggt aagctgcgcg gcggcctcct
     4561 gatgcgcgcc ctcgagctgc tcgttgagcc gcgcgaggtt ggtgacgatt attccgcgga
     4621 ccaactcggc ggtctcctcc accgagagat tctcgaaggc gtgatagggg acctcatcga
     4681 gcacgcggat gcacatcttc tgtatcccat gaaaattcac gctgtacttg gggagcgcct
     4741 tcatggtccc gctgatgacg atcggcagga tggggatctt tagcttcttt gcaagaatga
     4801 aggcgccgct cttgaacgcc ttcacggtcc cgtcgaacga acgcgtccct tccggaaaca
     4861 tgaacaccga actcccctcg cgcaggcgct cctcgcatgc ctgcatcatt tcctggacgc
     4921 tctccttgac cccgcgcgtg agcttgatat agcgattgag cgccatgttc cacccgatga
     4981 gcggaacctt gaacatctcg accttggaaa cccacttgaa atgaaaaaag agtcggaagg
     5041 acaccaggat atcaagctgc gactggtggt tcgagacgac catgtaggtc gcgccccggc
     5101 gaatcttctc gcgaccctct atcgtaatgc gccacggcgg catgatccag gtgtagaacg
     5161 aaccccagaa gcaggtgaac atatgaagcg ccctgaggcg ccgatcgaat ggggccgtaa
     5221 cggccttgat gaccagggcg atgacaaaca tggggataga ggtgaccgcg ataattacaa
     5281 gaaaaagaat ggaaacaaaa cgattcatca ttgcctctta aaaatgttcc ttgatcgcgt
     5341 ccgagcacgc cggaccggac gccattcgag cattcggggt gaaaccggta tattcgcctt
     5401 tgtacgcgtt cctgtcgcgt cacacataac gcgtggattt attcgagaaa acatccgccc
     5461 gcgcagtcaa gcccgccatg cgaccgaacc gacgccgcct ggtttgtcac ggttgtaaaa
     5521 cgctaccgcg cagcctcgcc ccgcgcgcgt tcccgaattt ttgcatacac gacgatgaat
     5581 ttcacgccat cgaagagcgc ggcaaaaagt ccctcgacgc cgtcgagaaa tccgagtttg
     5641 aaaaaatatc gccgcacaaa gcgcccgagc ggcttgatgc agcaataggc gatcgcgttt
     5701 ttcggacccg gtcgaaagcc gttggcgtaa atgaaattcg cctcgaaggt cgcgtaaaaa
     5761 tcgaacttct gcaaaaactg ggacaccgtc aggaaggggt gatgatgcat gtcgccgcgc
     5821 agccgcccgg tgcccccctg gatctcaagc ttctcgtgaa cgtgccggtt ggggaagcgc
     5881 gcagcgccgc gcttgaacag ccgcagctgc gtgtccggat acttgctccc gtgccgcaac
     5941 cagcgcccga agtagtgatt gcgccgtccc agctcgaagg cgacggcgcc ggacggcgat
     6001 cccacaacgc cgagcacctc gtcccgcagc gcctctggaa tgcgctcgtc gggatcgaga
     6061 tacaggatcc aatccccggt cgccttgtcc atggcgaagc tcttgttcac gttcaggttc
     6121 gggttgttgg gccgcgagaa ggttcggcag ccgtgccgct tcgcgaccgc gagcgagcgg
     6181 tcggcgcttt cgcaatcgac atagacgatc tcgtccgccc acgaaagcga cggcagcagc
     6241 tcctcgagat gcccggcctc attgtgcccg atgatggtta tgctgatggt catgacatgc
     6301 cttcgcgacc gatagaagaa tacaccgcaa gattgctggc aagaaatttt tcctccgtat
     6361 gacgcgcacg accggcctcg atggacgcgc gcgccgcgcg aaaggccttg ggctccgcaa
     6421 tatcctcgat ggcctccgcc atcgctcgcg gatcggccgg ggggaccacc cgccccccat
     6481 aggcgccgat cgtctccggg atggagccga tggcggtccc gacgaccggg atcccgcacg
     6541 cgaacatctc cagcgcaacg cggcagatcg cctccgagcc cagggagggg atcgcaccga
     6601 tgtcggtcgc ggcgaggatg tcgcgggcgt cgtactcgcg tccgataatc gtcacctgcg
     6661 acgcgccgat atcgagcgcg atgcgtcgca ggtcctcgat gcgcacgtcg atctcctcgc
     6721 cggttatgag aaaatggaac cgccccggat ttttcagata ctgataactc cgcacgaaat
     6781 gctcgacccc cttgacggcg tggagccttc ccagatgcgt gaccaccagg cgtttccggg
     6841 gaatgccgag cgccctgcgc acatcgacat tcgtgcgttg cgcgacgaag gcgcgccggt
     6901 cgatcgccag cgggatcgac gtgatgcggt cgacaggcac cccgagtgac gcgataaccg
     6961 gcaccaggtg cgcgccgggc acggtatagg cctcagcggt atgccggtac tgcaggcggt
     7021 tcaaaagatg cgtcttgggc ggattggttt cgctccgggt tcggacgatc ggcacgccca
     7081 tcccggcgcg cgcgacggcg gcgaaggtgt ggtcgcgcga aaaatgcgca tggataatgt
     7141 cgatgccgtg gccgcgcagc gcgcgccgaa agccggcgac attacgcgca agcgcccacg
     7201 gggcggaggc ggccaggttt acgtcctcaa cgacaggaat atccgccgcg cgggcgcgct
     7261 ccacgatcgg gcttcccggc agccccagga ccaccgcgtg gtgcccggcg gccgcgagcg
     7321 cccgggccag gaataacgca tagtgcgccg aggcattgta ccagcgcacc gacgccataa
     7381 ccaagaccga acgggcgccg tcggcgctca ttcgatcccg tccgcgacgg caaccctgtt
     7441 ttttcccgcg cgcttggcgc ggtacatcgc catatcggcc gccttgataa gatcgtgccc
     7501 gtccatgatg gacggcgaaa gcccggccac gccgatgctc accgtgaccc cctcgggcag
     7561 ccggttgctc gtcgccgtct tcttcgcgat catgcgcacg attttattcg caagcgacac
     7621 cccggcggcg agcttcgtgt gcggcagcac taccagaaac tcctctccgc cgtagcgccc
     7681 gatcttgtcg gtcttccgga cggtctcaac gagctggtcg ctgaccgtga tcaacagccg
     7741 gtcgccgttc ttgtgcccgt gctcgtcgtt gtagcgcttg aaattatcga tgtcgatgaa
     7801 cagcacgcac acgtcaaacc cataccgctg cgagagcagg aactccttct ccagctcgta
     7861 atggatgatg gcgtgcgagt agagcccggt gaggccgtcc atcatcgcgt cgttcagggc
     7921 gcgcatcagg ttctctttta cgaagatgac gatgtcctcg ctccgcccgg tccgcgtgaa
     7981 ataatccaca agggcggtct tgatggtcac cggacgattg agacaggccg ccatgtgccg
     8041 tcgattttca acaatatcga accagacgcg ctgggcgttg tcctcggcga tctcgtaatg
     8101 gagtatcacc tggagcaggg tggagtacgg tttgtccgtc tcgagcttga gcgattccag
     8161 gacgaaaccg aggatgtcgt catcgtcgat gtccgaaagg acgtcggact cgatcttttc
     8221 ccgggttgtc atgaggcgct tgatcatgac gatagttctc cggattattt ccggcccgtc
     8281 aagaccgaaa tttttttagt tgcattttac tcccctgcgc gcttcatacg acgcagacgg
     8341 acacgggcac tcgagagcac gcacacgcca tggcaaacgg aaacggcgca cccacgggac
     8401 tcatcgattt ctatctcgac ctggaggacg atcccgaata caccgggaaa accggcgaga
     8461 tattccggaa aatcctcacc gattattttt tcggcttcga gaccgacgcc aataaaagct
     8521 tcgcgctcat gctgggtaat ttcgaggcgc ccgacttcat cgcggccgcc gacagcctgt
     8581 tcaatctcga tctcgacgcc tttgcggact ggatcaacgg ggaaacgctc aacacctctc
     8641 tgggcggccg catcatgctc aacccgaagt atctcaaata cgcctatccc catcatcctc
     8701 ccgaatacaa taaactgccg cccaatgtgc aggtcgagct cttcgatcgc atcaagaaga
     8761 aaaaccgcga gttcatcgac gccttccatg gcatgatggc cgatcgcgcg gccgaccgcg
     8821 ggcggacgat cctcgcgctc gtcgcgatga ttctccgcga ggtgcacaag aaaacggaga
     8881 tgccgctcaa tcggctcacc caaaaggccg acgcggtgat ccgaacgtcc ttcgagtact
     8941 gcgacgagat tttccgcgcc tcgcagaagc agatgactga gctgggggac gacagcaagg
     9001 tgcgcgcctg cattaaaacg ttttttcccg tcaagcgcgc ccaggagatc gccgagatcg
     9061 gcggcgtgtt caagcaggag cttgaacgca tgaagcgacg gacccttcgc gcccagcaat
     9121 agcgcgcgcc gcgcaatcga cgctatcgaa gcatgtggat gatccgtccg ctcgcctgcg
     9181 agaacgcgag cgcgatcccc gcggaggacg cggcaaacat gcccgcacac agggccataa
     9241 ggccccagcc ctcgtccgcg cgtcgcggaa cggcgcctgc ggcggcgcat cccatgaccg
     9301 cccccagggc gcccgccgtc ccggcaagca cgcaccatgc gcccatgatc agcaccaccg
     9361 ccgatagccg catcgcatca ccgcccgaaa cacccgcacg ggcgcgctgg acgaccgttc
     9421 gataatccgg cgtcgcgctg cgtcgggcga ttcccggttg ttcgcgaaat tgtcgccgca
     9481 cctcctcgac gatcgcggtg ctgccggagc gcgtcattcg ctcaagcatt tccgcgatcg
     9541 cgtacgagcc cgcagcgcca agcacgaccc cggcgatcag gagcggatta aacggggaaa
     9601 ggtcaaatgc gcgcgcgagg tcgcgaaacc ccgcgagagc gatgaggaga tcgtcgggag
     9661 ccagaacaac gccatcggga aaaaccgcgt gcagcgcgac cgcaccggcc ttgagcgacg
     9721 atgcgcccgc catgcgcgaa agccagaatc gcgcctgctc gagcgacgcg ccgatcaacg
     9781 caatggcgac gaccaccgcc gcgcatccga gcgcgcatcg cactgcgtac tgcccgcgat
     9841 cggcgctgcc agaaagcatc ataagaccgc gccgcgcctc tgcggtatac ggctcgccct
     9901 tctgtgacac gatccacagc ggcaccgcgc cgcgcgcgat ccggggaagc gcggcgaatg
     9961 aaccgacggc catgccgagc gctccgaagg cgatcccgcc ccaaccgcca agcgcctccc
    10021 gaccgccgcc cgcactggca tagcaaacca cgagaagaat cccgatcatg agggcgggaa
    10081 catagcgcac gtgcacccat gcaatgtttc ctccattgaa cgggtgaacc tgaacggcat
    10141 cagcagtcct tttctgtgcg atgtcgatgg cgcacaggac aatagacatc gccccaatgc
    10201 ccatcgcaaa gcacgcaaac gaaaccgctg gcaccagacc gcgccaagcg agaatgccgc
    10261 cgcacgcaat cacgacgagc ccgccgagga aatccaatcc cgtacccgcc caccggcgaa
    10321 gcgggccctc cggcgataac gaaatcccaa gacaggcgac cgagagaaat ggaacaagca
    10381 cgacgccgca gcccatgatg atcggcgccg ccatatcgtc cggaaccggt cgcgacgccg
    10441 cgtgccgccc gatggtcgcg gcgatgagcg gaaacgccgt cgccagtgaa agcgtgacgc
    10501 gcacggccga cgaaagcacc gcataggcgc ccatgcatgg gatggcgacg cccgcgatgc
    10561 ctgcgccaat ggtctcggcg gccaggaccg gatttcgcgc atcgccgtcc tgcaggccct
    10621 tgatcttttt tcgaacatgc ccggcgccga cgcgtaccgc gtcggcgaat cggcccatcg
    10681 tcaaccgcgc gaagcacgcc tgggcgaccg agccgagccc gcaggcaagc aggagcgcca
    10741 tgacttccga tgcgcgccat cgggcgatcg cggcattacg gaacgccgcg ccgccgggcg
    10801 cctgtgcgag ccaggcgaca cgcccggcgc actcgccgcc ggcggcggcg cactcaccct
    10861 cgagcgcgcg ggagacgaac acgagccatg cgaaaagctc cagcagtaca aacccgatca
    10921 cgcacagcgc gacaatgaaa ttcgggagcg cggccacgcg tcgggcgtgc gcgagggatc
    10981 gccggagcgc gaacggcacc cggggcgccg ccagcattcc cgacaacacg ccgaccagtc
    11041 cgcacagcgc cgagagcatc cacccgaccg cgcatgcgat ggggatgagc caatgcgccc
    11101 atccggcggc gacaagcgcg ctcatgacgg ccgtcccgac aagaccggcc acgatcgtcg
    11161 cccgccccgc atgcgcgaga aattcgatct gcgccggtcg ggtatccgcg agcatccgcg
    11221 ccgccggctc ggagaccggc agtcgcacca gccagtacag aaatgccccc gcgccggcaa
    11281 gcgagcaaaa ccctgtcgcg gcgagtccat acagaaactg gttcgtcatg ggcgtcaccc
    11341 cttatacgcg aaaatgaaac ggcaggcgga gctcgtcacc ccgcctgccg tgagattgca
    11401 tgctagaccg aatgatccgg aatcacttct ccatcgaatt gtacaccagc tcggcgaggt
    11461 cgaacgattg aagattctgg atgtccagct cgttcgcgcc gtccgaaagc atggtgagac
    11521 agaacgggca ggccgtgccg atcttggtcg cgccgcttgc ctgcgcatcc ttggtgcgga
    11581 actggtttat ccgcgtcccg atgtgctctt cgatccacat acgcgcgccg cccgcgccgc
    11641 agcagaagct ctggccgtgg ttgcggctca tctcgacgaa ctccgtgccg gggatggcct
    11701 tgataatgcc gcgcggctgg tcgtagatct cgttgtagcg gccgaggaag caggagtcgt
    11761 gatacgtgac cttctcggcc agcgcatcct tgagcttgat ctgccccttc ttgatgatgt
    11821 ccatgatcac ctcggtgtga tgatagacct catacgcgca ctcgagcggg ttgccgtccg
    11881 agcccagacc caccttcgcg aacttgggat attccttctt gaggacattg tacccgtgcg
    11941 ggcaggtggt gatgatcttc ttgatgccgt aattcttaaa ggtctcgagg ttttgcgtgg
    12001 caagcgcgtg gaagagatac tcgttcccgg cgcgcagtgc ggaatcgccg cagcagccct
    12061 cttcgggccc caggatgccc accttgtacc cggccttctg gagcaccttc accagcgccg
    12121 tggcgacctt ctgattgcgc
//
LOCUS       DAORHF010000101        12125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4381_length_12125_cov_4.866611, whole genome shotgun sequence.
ACCESSION   DAORHF010000101 DAORHF010000000
VERSION     DAORHF010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12125
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4381_length_12125_cov_4.866611"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /gene="rsmA"
                     /locus_tag="PKJ16_09795"
     CDS             complement(<1..789)
                     /gene="rsmA"
                     /locus_tag="PKJ16_09795"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HNV47322.1"
                     /translation="MNKAELIARCADAGISPNRRLGQNFLFNETIIARIVAAAGIQPT
                     DRVLEIGPGMGTLTEAIAERAASVHAIEIDAGLARHLRDAFAHRDTVTISHADFLKSA
                     PVPGITKIISNLPYYCASEILFAIATGYANPETYVMLQREMAARIAARPGGKDYGALT
                     VTLGFYFSMERLFDIDKQSFHPAPEVTSTFIRLARTDIAAHDARWIERFHTVVKSAFW
                     GRRKPILGALVESPHARFVRDGVIAALASCMIPPNTRAERLSVDD"
     gene            complement(786..2993)
                     /locus_tag="PKJ16_09800"
     CDS             complement(786..2993)
                     /locus_tag="PKJ16_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47323.1"
                     /translation="MHNAVMGTGDTIGAYGAPAGGGVPLDFVELLPQVVFETDAAGII
                     LYVNRIASELSGYSMDELRSGKNILSFLTPECREIAVTNMTEVMAGRRPRTAEYTIIR
                     KDGSTFPVNIYSTAIIRDGNFAGLRGIIVDITDQKQTESALSQSEARFQTLIHNLPYG
                     IVLIAPDGSPEYLNPTFTRLFGYTPADIPSAQAWFERAYPDPVYRASMMALWSETDAE
                     KTASTTTDFVVRCKDGSDKSIQFHRVNIDGGRTILTCIDMTARVKAEEALRESKHELD
                     FITGNMIDLIAHAGPDGRYQYTSPSYQTVLGYSETELRAMSGFDIVHPDDIQRIWATY
                     IEAIINREPRIRYETRLRRKDGEYLPFEVTAKILYDDAGRLAGGILSARNLTERRAAA
                     RALRESEEKYRAIFANSSEGIFQSTPAGSYLIANPALAHMYGFSGPAELLREVTDIAA
                     QIYADPADRSRFIELLEKHGAVTNMEMRMRRRDGSLFWVSTNARAVRDAGGAIAYYEG
                     TSRDITERKQSRERIQDALREKEVLLREVHHRVKNNFQIISSLMNLQRQRIADEGLRS
                     HYGDAINRIAAMAAVHERLYRSADLSRVDLADYIADIAAELMTGFRRPDRPVALHCDT
                     APITVPVEIAIPCALIINEALTNSLKHAFPPDWRGDPEIRITLRRRGPGEVELALADN
                     GVGIPDQPPGPAQSLGLQIIPLLARQINGRHSIERGDGACHRIVFANIPAAAR"
     gene            3112..3906
                     /gene="flgG"
                     /locus_tag="PKJ16_09805"
     CDS             3112..3906
                     /gene="flgG"
                     /locus_tag="PKJ16_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HNV47324.1"
                     /translation="MMRSLWTAASGMIGQQFNIDTISNNLSNVNTTGFKKSRAEFEDL
                     IYQTMLMAGTPATEVSEVPTGIQVGHGVKTSASQKMFMQGSLQNTENKLDLALEGEGF
                     FKVQLYDGTFAYTRDGSWKIDSNRQVVTSNGYLLEPPLILPENFIHDSLAISQDGRIT
                     VKLVGEDDPIDVGQLEIYRFVNPAGLASIGGNLYKTTPASGPEIAGQPGLGGMAKTHQ
                     GFLEMSNVKVVEEMVNMIVAQRAYELNSKAILTSDNMLGTAIGLKR"
     gene            3908..4426
                     /locus_tag="PKJ16_09810"
     CDS             3908..4426
                     /locus_tag="PKJ16_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella basal body P-ring formation protein
                     FlgA"
                     /protein_id="HNV47325.1"
                     /translation="MLRAALIVAFLITALGVAAAEVRLLLYPTAQRGDAPLALSDVAR
                     VEAGAATDAIGAILIPGELCRDGLLDRTEIAALVARLTNDLVFIEGNAVRIVRPNGAR
                     DEAPAGPARDVGIKAGERITVVVRKNGVAIELPGVALGDGRAGETVSVRLKGAARLTG
                     RIVEGGRVEVTL"
     gene            4423..5103
                     /locus_tag="PKJ16_09815"
     CDS             4423..5103
                     /locus_tag="PKJ16_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HNV47326.1"
                     /translation="MRRNAYADDATGAIVGFLIVSFVLVFILSAAAVFPKSLWTDRNV
                     YSSAASLKVGDILIVKIEDVSQMKFSVTVADNNTFNITANPDQGITPFLPKVSTDRKS
                     ASTGKTDFSGKNNMLVSIAARVTGRRDDGKFTVDGRRDYTFHGVRSSFRVRGIIDPAL
                     VKGKTVLADDIADFRMDIVGGKSGAPVDLKREPLKEGESAAFKLTEEEKQRIILDYVK
                     KMLQEITR"
     gene            5108..6121
                     /gene="flgI"
                     /locus_tag="PKJ16_09820"
     CDS             5108..6121
                     /gene="flgI"
                     /locus_tag="PKJ16_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HNV47327.1"
                     /translation="MKRIPAFIVCMMMLALAARAEVMVKLRDISYVDGLKENQLMGFG
                     IVVGLPGTGDTRSALTKTSLRNLLKNMGIEGDDIGSKNTAAVMVTAMLPAFARVGDRV
                     DITVSSIGDAKSLEGGILVQSPLRGADDRVYVAAQGTLSMDGAGAPGGRTKTVKTVGS
                     ISGGGIVERESLPEYGADSAIQVVLENWDFTMASRIVKALNDAFPESKAAIAKGGKIK
                     LVVPKETSVDEFIAKIEAIEVPASARARVVVDERSGTIVMGGAVRISEVVVSKEGMTV
                     RIEKKDKGSAGTTSVAHIKDVATVKDLVDALGSIGASTRDIIAILKAMDEAKALHAEL
                     IIR"
     gene            6132..6620
                     /locus_tag="PKJ16_09825"
     CDS             6132..6620
                     /locus_tag="PKJ16_09825"
                     /inference="COORDINATES: protein motif:HMM:NF021638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HNV47328.1"
                     /translation="MITQNDLSMIMDARRTALESGTAQGVKKRVDTSGKHADFASVMH
                     QVLDVEGLAAGPGKRASQSAGMRMPEANHTFRAPHNAIPLDRKPVDTRLMDACVEMES
                     LFVSKMLKEMRKTVQKGEMLHGGFAEEIFEDMLYDNYALEMSKTANMGLAKMLYGELS
                     KK"
     gene            complement(6642..7100)
                     /locus_tag="PKJ16_09830"
     CDS             complement(6642..7100)
                     /locus_tag="PKJ16_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47329.1"
                     /translation="MAIDTTLNVHESTLRRIDRITSITGRARNRVIVDLLTRVMSDRG
                     KQFATGCTVRYQRRDNKEKWRTVHVRLSEDEVDYFRDLRGVYKRSESLLLAEAAREYL
                     SDIIREALRGVKSDNHPFQNYVLSRHEAEGLIFWTICWGFPPTPQNHFKL"
     gene            complement(7257..8267)
                     /locus_tag="PKJ16_09835"
     CDS             complement(7257..8267)
                     /locus_tag="PKJ16_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47330.1"
                     /translation="MSLRTGLFRLISMFVIAASAFMFSCDEPQDSNNVYVAGWYNKGD
                     IYYPCYWKNGTRVDLGSRSSTYSSIAVSGSDIFVAGNMNFQPTVWKNTASNKTTLETS
                     FSMAMGIALSGNDVFVSGNYFLPSDIMIACYWVNGSRVELENVESLAHNMMIQNGDIY
                     IAGAVGPIGSSVPCYWVNGSRVELANDTPTAFARSVFSTGNDVYAAGGADDANGSRRA
                     VIWKNGVKTVLHNGDSEAFSVFVNGNDVYVAGHAWINGIRTACYWKNGVRTNLGDGSK
                     HSDAYSVYFFGGNVYVSGDYIDNGKYACVWRNDKNNRYKLSDGGVTSHDAAGTSIFVT
                     AK"
     gene            complement(8435..9394)
                     /locus_tag="PKJ16_09840"
     CDS             complement(8435..9394)
                     /locus_tag="PKJ16_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HNV47331.1"
                     /translation="MLQKIINAVKIYFSMLNMTPGSAPPIFRKPTVDVPQAKPTPFWV
                     HVLLFAITFVTTTASGVVMCAQSDDFIAEIPRVGIPYALAIMTILLAHELGHYYAAKR
                     FDVATTRPFFIPFIPPVGTMGAVIRIKSKIHDKRTLLYIGAAGPMISFTLSLVATVVG
                     LLLSEVKAFPAAPPGGELIELGDSIILLGLTRLIHGEIPPGFGMIISPLTWAGWIGFL
                     VTSLNLMPIGQLDGSHILYALIGRRQVYAGWLAFAGLVALAVVWPGWIVWIALALIVL
                     MIGHPPIEDGEPITIGERIAGWFCIVILIITFIPQPVNILPIK"
     gene            complement(9843..10130)
                     /locus_tag="PKJ16_09845"
     CDS             complement(9843..10130)
                     /locus_tag="PKJ16_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47332.1"
                     /translation="MKDILIVHGGTMKALRFVERIANGRIVVAKLDKYSGMDAEVIII
                     PLIETKSNGKNATTKKTASMRGCLKKYANPLLVQDEKEAWATAAGKKHAGL"
     gene            complement(10202..11611)
                     /locus_tag="PKJ16_09850"
     CDS             complement(10202..11611)
                     /locus_tag="PKJ16_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47333.1"
                     /translation="MSLLRYISALILLAVLATPAFVGAQTNGGSKADMPDGAASPDRP
                     DITETKKDANDEEAKVFSFKAQFKELFTYTDTIERSFAVNTYIDESTGEVGYSVKEKA
                     RNKSLFANLMRLRLSPEIKGGERFLLHVDLDNEIISSDYMKSREFDQYWRPSDYNDFA
                     DLSCEPVYDDMSYYRMKLHRAYAKILLDKFTATIGRQQIRFGSGRLWNPLDILNPISP
                     TYVEGPEDQKGTDALRLEYYPFDKTELGLIIDPKRMDNDEDIGKLNSDNTNVLARFKT
                     SLYETEIALMGGRVSHRLVGGADASTHLLDGLLRGSVLYAKPDPGREYAQASAGYEYN
                     FSFGLYFLIEYFYNENGMNYNSNLANAYERFVMEGMSEAIYRKLANQLLTWNQHYGGI
                     ALGYDITPLVRGELFSIYDFQGKGLYAAPSLKYNIVQNADLAIGVMKAYRMGISRTPT
                     DFESLINHPYFFFSLVAYF"
     gene            complement(11622..>12125)
                     /locus_tag="PKJ16_09855"
     CDS             complement(11622..>12125)
                     /locus_tag="PKJ16_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV47334.1"
                     /translation="GYSPRQLSDLRLRKIGFIFQAYNLIPVLSAIENVEYILLLQGME
                     KTERRRRSAAMLAEVGLEGLTDRRPHQLSGGQQQRVAVARAIVSEPDIVLADEPTANL
                     DQKTGAALLDLMHELNRTKGITFLFSTHDGMVMERAERLISLRDGVVEHDRVKRRAKP
                     RSAKRKR"
BASE COUNT         2295 a         3487 c         3925 g         2418 t
ORIGIN      
        1 gtcgtcgacg gagaggcgct cggcgcgcgt gttgggcggg atcatgcacg aggcgagcgc
       61 ggcgatgacc ccgtcacgga cgaagcgggc gtgcggcgat tccacgagcg cgccgagaat
      121 gggcttgcgg cggccccaga aggcggactt gaccaccgtg tggaagcgct cgatccagcg
      181 cgcgtcgtgc gccgcgatgt ctgtgcgcgc gagtcggatg aaggtggagg tgacctccgg
      241 cgcgggatga aacgattgct tgtcgatgtc gaagagccgc tccatggaga aatagaaccc
      301 gagcgtcacg gtgagcgcgc cgtagtcctt ccccccgggg cgcgcagcga tgcgcgcggc
      361 catctccctc tggagcatca cgtaggtctc ggggttggcg tagccggtcg cgatcgcgaa
      421 gaggatctcc gaggcgcaat agtaggggag attggatata atttttgtga tcccggggac
      481 gggcgcgctc ttcaggaagt ccgcgtgcga tatggtcacg gtgtcccggt gcgcgaaggc
      541 gtcgcggaga tgtcgcgcga ggcccgcgtc gatctcgatc gcatgcaccg acgccgcgcg
      601 ctcggcgatc gcctcggtga gagtccccat gccgggcccg atctccagca cgcggtcggt
      661 cggctgtatc ccggcggcag cgacgatccg cgcaatgatg gtttcgttga aaaggaagtt
      721 ctgtcccagt cgacggttcg ggctgatgcc ggcgtcggcg cagcgcgcga tgagttcggc
      781 cttgttcatc gggccgccgc ggggatgttc gcaaagacga tacggtggca ggcgccgtcc
      841 ccgcgctcga tgctgtggcg tccgtttatc tgtcgcgcaa ggagcgggat gatctgcagg
      901 ccgagcgatt gggcgggtcc cggcggttgg tcggggatgc cgacgccgtt gtccgcgagc
      961 gcgagctcga cctcgccggg tccccggcgg cggagcgtta tgcgaatttc cggatcgccg
     1021 cgccagtccg gcgggaaggc gtgtttgagc gaattggtga gcgcctcgtt gatgatgagg
     1081 gcgcagggaa tggcgatctc gacgggcacc gtgatgggcg cggtatcgca gtgaagcgcg
     1141 accgggcggt ccggacgccg gaagccggtc atgagctcgg cggcgatgtc cgcgatataa
     1201 tcggcgaggt ccacgcgcga gaggtcggcc gagcggtaga ggcgctcgtg caccgcggcc
     1261 atggcggcga tgcggttgat ggcgtcgccg tagtgggagc ggagcccctc gtccgcgatc
     1321 cgctggcgct gcaggttcat gaggctcgaa atgatctgga agttgttctt gacccggtga
     1381 tggacctcgc ggagcagcac ctccttctcg cgcagggcgt cctggatgcg ctcgcgcgat
     1441 tgtttgcgct cggtgatgtc ccggctggtg ccctcgtagt aggcgatcgc gcccccggcg
     1501 tcgcggacgg cgcgcgcatt ggtcgaaacc cagaagagcg atccgtcgcg ccgccgcatc
     1561 cgcatctcca tgttcgtaac ggcgccgtgt ttctcgagaa gctcaatgaa gcgcgaacgg
     1621 tccgcggggt ccgcgtagat ctgcgcggcg atgtcggtca cctcgcgcag gagctcggcc
     1681 gggccgctga agccgtacat gtgcgcgagg gccgggttcg cgatgagata actccccgcc
     1741 ggcgtggatt ggaagatgcc ctccgagctg ttcgcgaaga tggcgcggta tttctcctcg
     1801 ctctcgcgga gtgcgcgagc ggccgcgcgg cgctcggtaa ggttgcgcgc cgagagaatg
     1861 cccccggcga ggcgaccggc gtcatcgtac aggattttgg cggtcacctc aaaagggagg
     1921 tactcgccgt ccttccggcg cagcctcgtc tcgtatcgga ttcgcggttc acgattgatg
     1981 attgcctcga tgtaggttgc ccagatgcgc tgaatgtcat cgggatggac gatatcgaat
     2041 ccggacatgg cgcgcagctc ggtttcgcta tagccaagca ccgtttgata cgagggcgag
     2101 gtgtactgat accgcccgtc gggcccggca tgggcaatga ggtcgatcat gttgccggtg
     2161 atgaaatcga gctcgtgttt gctctcccgg agcgcctcct cggccttcac gcgcgccgtc
     2221 atgtcgatac aggtgaggat ggtcctgccg ccgtcgatgt tcacccggtg gaactggatg
     2281 ctcttgtcgg agccgtcctt gcagcggacg acgaaatcgg tagtggttga ggccgtcttc
     2341 tcggcgtcgg tctcgctcca cagcgccatc atggacgcac ggtagacggg gtccgggtat
     2401 gcgcgctcga accacgcctg ggcgctgggg atgtccgctg gcgtgtagcc gaacagccgg
     2461 gtgaacgtcg gattgaggta ttccggcgag ccgtcgggtg cgatgaggac gatgccgtac
     2521 ggaaggttgt ggatgagcgt ctggaagcgc gcttcgctct gcgagagggc gctctcggtc
     2581 tgtttctggt cggtgatgtc cacgatgatg ccgcgcaggc ccgcgaagtt tccgtcgcgt
     2641 atgatggcgg tcgagtagat gtttaccggg aaggtgcttc cgtccttgcg aatgatcgtg
     2701 tactcggcgg tgcgcgggcg acgtcccgcc atcacctcgg tcatgtttgt aacggcaatt
     2761 tcgcggcatt cgggcgttag aaaggaaagg atgttttttc cggatcggag ctcgtccatg
     2821 ctgtagccgg acagctccga cgcgatgcgg tttacgtaga ggatgatccc ggcggcgtcg
     2881 gtttcgaaca cgacctgcgg cagcagctcc acgaaatcga gcgggacgcc gccacccgcc
     2941 ggggcgccgt atgcgccgat tgtatcgccc gtgcccatga cggcattgtg catgtccggc
     3001 gcgtttcggc aagcagaaat ggcgcatttt gttgcttcag gcattgcgcg atttgtccga
     3061 tagggaacgg tagaagatgt atcattcccg caggttcgcg aggtacgcgc catgatgaga
     3121 tcgctctgga cggccgcttc cggcatgatc ggccagcagt tcaatatcga tacgatatcg
     3181 aacaaccttt ccaatgtgaa taccaccgga ttcaagaaga gccgggccga gttcgaggac
     3241 ctgatctatc agaccatgtt gatggcggga acgcccgcga ccgaggtgag cgaggtcccc
     3301 accggcatcc aggtgggcca cggcgtgaag acctcggcct cgcagaagat gttcatgcag
     3361 ggcagcctcc agaacaccga aaacaagctc gatctcgcgc tcgagggcga ggggttcttc
     3421 aaagtgcagt tgtatgatgg cacgttcgcc tatacgcgcg acggttcctg gaagatcgac
     3481 tccaaccgcc aggtggtcac ctccaacggc tacctgctcg agccgccgct catcctgccg
     3541 gagaatttca tccacgattc gctggccatc agccaggacg gccgcatcac cgtgaagctg
     3601 gtgggcgagg acgatcccat cgacgtgggc cagctcgaga tctatcgctt cgtgaacccg
     3661 gcgggcctgg ccagcatcgg cgggaacctc tacaagacca cgccggcatc cggccccgag
     3721 atcgcgggcc agccgggcct gggcggtatg gccaagacgc accagggctt cctcgagatg
     3781 tccaacgtga aggtggtcga ggagatggtg aacatgatcg tggcgcagcg cgcctacgaa
     3841 ctcaactcca aggcgatcct cacctccgac aacatgctcg gcacggcgat cgggctcaag
     3901 cggtaagatg ctgcgcgcgg ccctcatcgt cgcgtttctc ataacggcgc tcggcgtcgc
     3961 cgcggccgag gtgcggctgc tgctctatcc caccgcgcag cggggcgacg cgccgcttgc
     4021 gttgtcggac gtcgcccgcg tggaggccgg cgcggccacc gacgcgatcg gcgcaatcct
     4081 gattcccggc gagctctgcc gcgacggatt gctggaccgc accgagatcg cggcgcttgt
     4141 cgcgcggctc acgaatgatc tcgtgttcat cgagggtaac gccgtgcgca tcgtccggcc
     4201 gaacggcgcg cgcgacgagg cccccgcggg gccggcgcgg gacgtcggga tcaaggcggg
     4261 cgagcggatc accgtggtcg ttcgcaagaa cggcgttgcc attgagcttc ccggcgtggc
     4321 gctgggcgac gggcgcgcgg gcgagactgt ttccgtccgc ctgaagggcg ccgcgcggct
     4381 caccggccgg atcgtcgagg gcggccgggt ggaggtgacg ctgtgaggcg gaacgcatat
     4441 gccgatgacg cgaccggcgc catcgtcggg tttctcatcg tgtcattcgt gctggtgttc
     4501 atcctgtcgg cggccgccgt gttcccgaaa tcgctctgga ccgaccgcaa tgtctattcc
     4561 tccgccgcga gcctcaaggt gggcgacatc ctcatcgtga agatcgagga cgtctcgcag
     4621 atgaaattct ccgtcaccgt tgcggacaac aataccttta atatcaccgc caaccccgat
     4681 caggggatca ccccgttttt accgaaggtg tccaccgacc gcaagagcgc gtccaccggc
     4741 aagacggact tctccggaaa gaacaacatg ctggtgtcga tcgccgcgcg cgtaacgggg
     4801 cggcgcgacg acggcaagtt cacggtggac ggccggcgcg actacacctt ccatggcgtg
     4861 cgcagcagct ttcgcgtccg cggcatcatc gatccggcgc tcgtgaaggg gaagaccgtc
     4921 ctcgccgacg acatcgccga cttccgcatg gacatcgtgg gcggcaagtc cggcgcgccg
     4981 gtggacctga agcgcgagcc gctcaaggag ggcgaatcgg ccgccttcaa gcttaccgag
     5041 gaggagaagc agaggatcat cctcgactac gtgaaaaaaa tgctccagga gatcacccgc
     5101 taacggcatg aaacggatac cggcattcat tgtgtgcatg atgatgctcg cgctcgccgc
     5161 gcgcgccgag gtgatggtga agcttcgcga catctcgtat gtggacggcc tcaaggaaaa
     5221 ccagctcatg ggcttcggga tcgtcgtggg cctgccgggg accggcgata cgcgatccgc
     5281 gctcacgaag acgtcgctgc gcaatctcct gaagaacatg ggcatcgagg gcgacgacat
     5341 cggctcgaag aacaccgccg ccgtgatggt cacggcgatg ctgccggcct tcgcgcgcgt
     5401 gggcgaccgc gtggacatca cggtatcgtc catcggagat gcgaagtcgc tcgagggcgg
     5461 gatcctggtg cagtccccgc tgcgcggcgc ggatgatcgg gtgtacgtgg cggcgcaggg
     5521 gacgctctcc atggacggcg cgggggctcc gggcggacgg acgaagaccg tgaagaccgt
     5581 ggggagcatc tccgggggcg ggattgtgga gcgcgaaagc ctgcccgagt acggcgccga
     5641 cagcgccata caggtggtgc tcgagaactg ggactttacc atggccagcc gcatcgtgaa
     5701 ggcgctcaat gacgcgtttc ccgaatcgaa ggcggcgatc gccaaaggcg gaaaaatcaa
     5761 actggtggtc cccaaggaaa cgagcgtgga cgagttcatc gcgaagatcg aggcgatcga
     5821 ggttccggcc agcgcgcgcg cccgggtcgt ggtggacgag cgttccggca ccatcgtgat
     5881 gggcggcgcc gtgcggatct ccgaggtggt tgtgagcaag gaggggatga ccgtccgcat
     5941 cgagaagaag gacaagggga gtgcgggcac cacgtcggtt gcgcatatca aggatgtcgc
     6001 cacggtgaaa gacctggtgg acgcgctggg ctccatcggg gcgtccaccc gtgatattat
     6061 cgcgatactc aaggccatgg acgaggcaaa ggcgctccac gcggagctta tcatacggta
     6121 gtgaggcgag catgattacc caaaacgacc tgtcgatgat catggacgcg cggcgcacgg
     6181 ctctcgagag cgggacggcg cagggcgtga agaagcgcgt cgatacatcc ggaaaacacg
     6241 ccgatttcgc gagcgtgatg caccaggtgc tcgacgtgga gggtcttgct gcgggtcccg
     6301 ggaaacgcgc gtcgcagtcg gcggggatgc gcatgcccga ggcgaaccac acattccgtg
     6361 cgccgcataa cgcgatcccg ctggaccgca agcccgtgga cacgcgcctc atggacgcct
     6421 gcgtggagat ggagtcgttg ttcgtgtcca agatgctcaa ggagatgcgc aagaccgtcc
     6481 agaaggggga gatgctccac ggcggtttcg ccgaggagat cttcgaggat atgctgtacg
     6541 acaactatgc gctggagatg tcgaagaccg cgaatatggg gctcgcgaag atgctgtacg
     6601 gcgagctgag caagaagtga cgcatttcgg cgatgcacgg gtcagagctt gaagtggttt
     6661 tggggggttg gggggaatcc ccagcagatg gtccagaaaa tcaatccttc cgcctcatgc
     6721 cgtgacagaa cgtagttctg gaacggatgg ttatcgcttt tcacaccgcg cagcgcctcc
     6781 ctgataatat cgctcagata ttcacgggcg gcctccgcca gtagcagcga ctccgaccgc
     6841 ttatacaccc cccgcagatc ccgaaagtaa tcaacctcat cctcgctcaa tcgaacatgg
     6901 accgttcgcc atttctcctt gttgtcgcgc cgttgatagc gcaccgtgca gcccgtcgcg
     6961 aattgcttgc cccgatccga catcacccgt gtaagcaaat cgacgatgac ccgattgcgc
     7021 gcgcggcccg ttattgatgt gatcctgtcg atgcggcgca gggtgctctc gtgaacgttg
     7081 agcgtggtgt cgattgccat gtggatatcc tccggtcgta tctgtacgta ttacgaagcc
     7141 gatcggaaaa aattgaaacg cggtccatca tgcgggagtt caattgcggg acggcacggc
     7201 ggaaaacaaa acgggccgca cggaggcgac ccgtcgttgc gggaccggag taatgattac
     7261 ttcgcggtca cgaatatcga ggtgccggcc gcatcgtgcg aggtcacgcc gccgtcggaa
     7321 agcttgtagc ggttgttctt gtcgttgcgc catacgcagg cgtactttcc gttgtcgatg
     7381 tagtcgccgc tcacatacac attcccgcca aagaaataca cgctgtaggc gtcggagtgc
     7441 ttggagccgt cgcccaggtt ggtgcgcacg ccgttcttcc aatagcaggc ggtccgaata
     7501 ccgttgatcc acgcatgtcc ggccacgtat acgtcgtttc cgtttacgaa caccgagaag
     7561 gcctcggagt cgccattgtg caggacggtc tttacgccgt tcttccagat gaccgcgcgg
     7621 cgcgagccgt tcgcgtcgtc ggcgccgccg gcggcgtata cgtcgttgcc ggtcgagaac
     7681 accgagcggg cgaaggcggt cggagtgtcg tttgcgagct cgacgcgcga gccgttcacc
     7741 cagtagcagg ggacgcttga cccgatcggt cccacggcgc cggcgatgta gatgtcgcca
     7801 ttctgaatca tcatattgtg cgccagcgat tcgacgttct cgagttccac gcgcgaacca
     7861 ttcacccagt agcaggcgat cattatgtcg gagggcagga agtagttacc gcttacgaag
     7921 acgtcattgc ccgaaagggc gatccccatc gccatggaga aggaggtctc aagcgtggtc
     7981 ttgttcgatg ccgtgttctt ccagacggtc ggctggaagt tcatgtttcc ggcgacgaag
     8041 atgtcgctgc ccgataccgc gatggaagaa taggtcgatg atctcgatcc gagatcgacg
     8101 cgcgtgccgt ttttccagta gcagggataa tagatgtcgc ccttgttata ccagcctgcg
     8161 acgtacacgt tattcgaatc ctgcggctcg tcgcacgaga acatgaatgc gcttgcggcg
     8221 atgacgaaca tcgaaatgag cctgaaaagc ccggtgcgaa gtgacatgtg aaactcctct
     8281 gacgtatcca gtgatatccg aatgatgttg cggcgcatga acgccgttct cattcgtgtt
     8341 gatggcaggc ttaaaaattt ggacggacgg tcaatactta atgcgaaaaa atattccaaa
     8401 ccttcgggac ggttgaaaaa gaaagaggct ggaattactt aatcggaagt atgttaaccg
     8461 gctgggggat aaaggtgatg ataagaatga cgatgcagaa ccatccggct atccgttcgc
     8521 cgatagttat cggttctccg tcttcaatcg ggggatggcc gatcatgagc acgatgagcg
     8581 caagcgcgat ccacacgatc caccccggcc acacgaccgc aagggccaca agaccggcga
     8641 atgcgagcca gcccgcatag acctgccgcc gcccgatgag cgcatagaga atatggctgc
     8701 catcgagctg gccgatgggc atcagattga ggctggttac caggaaaccg atccagcccg
     8761 cccaggtgag cggcgatatg atcatgccaa agcctggggg aatctcgccg tgaataagtc
     8821 gcgtgaggcc taaaagaatg atcgaatcgc ccagctcgat gagctcgccg ccgggaggcg
     8881 cggcgggaaa ggcctttacc tcggaaagga gcaatccaac gacagtcgcg acaaggctga
     8941 gggtaaagct gatcatcggt cccgctgcgc cgatgtacag gagcgttcgc ttgtcgtgaa
     9001 tcttcgattt tatccggatc acggcgccca tggtgcccac cggcggaatg aaggggatga
     9061 agaacggccg cgtcgtcgca acgtcgaatc gttttgcggc atagtaatgg cccagctcat
     9121 gcgcgagcag gatcgtcatg atggccagcg cataggggat ccccacccgc ggaatctcgg
     9181 cgatgaaatc gtcggattgc gcgcacatga ccacgcccga cgccgtcgtc gtcacaaacg
     9241 tgatcgcaaa cagcagcaca tgcacccaga acggcgtcgg cttcgcctga gggacatcaa
     9301 ccgtcggttt ccgaaagatc ggcggtgcgc tgcccggcgt catgttgagc atcgagaagt
     9361 agatttttac ggcgttaatt attttttgga gcatattgat ccgggacgcg catagttttc
     9421 tgctggacta atcatcggtg tcgcttcaag gtttaagttt ttttgattct cttctcgatg
     9481 aagatttcat tcgtgacaac tgtttcaaga gttttttatc gaacgtcact attgtatgct
     9541 ggtgtttttt ctgatagccg cacagaaaag cgtcaacgat gtcaagtttc gtgtctttat
     9601 atacatcaag agcatggaag gctactgtct tatcgacggt ttgaatagta ttgtgccgta
     9661 aaagatttct gatgcttgtt gatattaccc ttcggggtat gcgatatacc ttttcgagca
     9721 catatacaat ctcggcgaca acctcaaacg gaatatatac gttctcgttg tcgattattt
     9781 ttgcagcaat ggatgcctgt tccggaatgt catcaagtat atatcggagt actacatttg
     9841 cgtcacaaac ccgcatgctt ttttccggct gccgtcgccc atgcctcctt ctcgtcctgc
     9901 acaaggagcg gattggcgta ttttttcaag caccctcgca tgcttgccgt ttttttcgtc
     9961 gttgcgttct ttccgtttga tttggtttcg atgagcggga tgatgattac ctcggcatcc
    10021 atccccgagt acttgtcgag tttcgcaaca acgatcctgc cgtttgctat tctttctacg
    10081 aatcgtaagg ctttcattgt gccgccgtga acaatcaaga tatctttcac tatggcaaat
    10141 aaaccggctc gaatgcaaat atgtcaatcc cattttccag gtctgatccc gccgcgtccc
    10201 ctcagaaata cgcgacgagc gagaagaaaa aatacgggtg attgatgagg ctctcgaagt
    10261 ccgtcggcgt gcgcgaaatc cccatccggt atgccttcat gaccccgatc gcgaggtccg
    10321 cgttctgcac gatgttgtac ttgagcgacg gcgctgcata gaggcccttt ccttggaagt
    10381 cgtatatcga gaagagctcc ccgcgcacga gcggggtgat gtcgtacccg agcgcgatgc
    10441 cgccgtagtg ctggttccag gtgagcagct gattggcgag cttgcggtag atcgcttcgc
    10501 tcatgccctc catcacgaac cgttcgtagg cgttggcgag gttcgagttg tagttcatcc
    10561 cgttctcgtt gtagaagtat tcgatcagga agtacaggcc gaaggagaag ttatactcgt
    10621 agccggcgct cgcctgggcg tactcgcgcc cgggatcggg cttggcgtaa agcaccgatc
    10681 cgcggaggag cccgtcgagc aggtgggtcg atgcgtcggc gccgcccacc aggcgatgcg
    10741 agacgcgccc gcccatgagc gcgatttctg tttcgtacaa cgacgtcttg aagcgggcca
    10801 gcacgttggt gttgtcgctg ttgagcttcc cgatgtcctc gtcgttgtcc atgcgcttcg
    10861 ggtcgatgat gagcccgagc tccgtcttgt caaagggata gtactcgaga cgcagcgcgt
    10921 cggtcccctt ctggtcctcg ggcccctcga cgtacgtggg cgagatcgga ttgaggatgt
    10981 ccagcggatt ccacaggcgg ccgctgccga agcggatctg ctgccgcccg atggtcgcgg
    11041 tgaacttgtc cagaaggatc ttggcgtacg cccgatggag cttcatccgg tagtagctca
    11101 tgtcgtcgta gaccggctcg caggagagat ctgcgaaatc gttgtagtct gacggacgcc
    11161 agtactggtc gaactcgcgg ctcttcatgt aatccgaact gatgatctcg ttgtcgagat
    11221 cgacgtgcag aagaaagcgc tccccgccct tgatctcggg cgaaaggcgc agccgcatga
    11281 gattcgcaaa gagcgacttg ttgcgcgcct tctctttcac gctgtagccg acctcaccgg
    11341 tcgactcgtc gatgtacgtg tttaccgcga aggagcgctc gatggtgtcc gtgtaggtga
    11401 acagctcctt gaactgcgcc ttgaacgaga acactttcgc ctcctcgtca ttcgcatcct
    11461 tttttgtttc ggttatgtcg ggccgatccg gcgatgccgc gccatccggc atgtccgcct
    11521 tcgaaccgcc gttcgtctgt gcgcccacga acgcgggcgt cgcgaggacg gcgagcagaa
    11581 tcagcgcact gatgtaccgc aagagactca tgcaggatcg ctcaccgctt ccgtttcgcg
    11641 gatcgaggtt ttgcccgccg cttcacgcgg tcgtgctcga cgacgccgtc gcgcaggctg
    11701 atgaggcgct ccgcgcgctc catcaccatg ccgtcgtggg tcgagaagag gaaggtgatt
    11761 ccttttgtac gattgagctc gtgcatcagg tccaggagcg ccgcgcccgt cttctggtcc
    11821 aggttggccg tgggctcgtc ggccagcacg atgtcgggct cggagacgat ggcgcgcgcg
    11881 acggccacgc gctgctgctg tccgccggag agctggtgcg gccgccggtc cgtcagtccc
    11941 tccaggccca cctcggccag catcgcggcc gagcggcggc ggcgctcggt cttttccatg
    12001 ccctgtaaaa gcaggatgta ctcgacgttc tcgatcgcgc tgagcaccgg gatgaggttg
    12061 tacgcctgga agatgaagcc gatcttccgc aggcgcaggt ccgagagctg ccgcggcgaa
    12121 tagcc
//
LOCUS       DAORHF010000102        12007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4445_length_12007_cov_5.771252, whole genome shotgun sequence.
ACCESSION   DAORHF010000102 DAORHF010000000
VERSION     DAORHF010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4445_length_12007_cov_5.771252"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /locus_tag="PKJ16_09860"
     CDS             <1..1437
                     /locus_tag="PKJ16_09860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HNV47335.1"
                     /translation="SLLASVRVDCIAIDEAHCISEWGHDFRPEYRQMAAFRAGFPDAV
                     CVALTATATEAVRRDIVESLRLRDAGVYVASFNRENLFFQVLPKDDAYAQLAAFLSAF
                     PGQSGIVYCFSRNGVDALAARLADAGHSVLPYHAGLEDEVRKQNQERFIRDEVQIIVA
                     TIAFGMGIHKSNIRFVVHYDLPKSIESYYQETGRAGRDGLPAHCLLLYSYGDIHKIRF
                     FINQKEDDRERANAERHLRALVDYAEATGCRRAPLIAYFGEAYEHDDCGMCDNCTQPP
                     APGEDLTVPAQKLLSCVKRVNERFGVNHIIDVLRGSESHKILSYDHHRLSTYGIGADY
                     SKKQWLHLARQFIRQGLMVQDMENFGVLTITRLGYDVMRGDATVRGTIINDRRAVFAG
                     GKSAQADYDRELFERLQKLRMALASAGGVPPYVVFSDRTLSEMASRYPRDRAALLDIH
                     GVGEHKCERYGDEFLVMIRDYCAERNID"
     gene            complement(1454..1921)
                     /locus_tag="PKJ16_09865"
     CDS             complement(1454..1921)
                     /locus_tag="PKJ16_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47336.1"
                     /translation="MNTNPLIGFIRTLGLIMSGRVLFLGSSVGKQLAMPDGRVFTVFR
                     HVKIRPKGISPDPEAVFLVRFKPRRMSDRANRVFSLLPMLVFMGFRGFRQKYWAVEEG
                     TGLCQGLYEWQTMADAGNYANSIAMRFMRNRSVPESVSFEIIDQRAARYWAFG"
     gene            complement(1939..2343)
                     /locus_tag="PKJ16_09870"
     CDS             complement(1939..2343)
                     /locus_tag="PKJ16_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV47337.1"
                     /translation="MKYILDTNICVYALKNMFPSIAAHLKSVRPSDVAIPTIVEAELL
                     LGAYKSSAREATIRAVSLFLEPITRIPFGEREAQCYADIRAGLEKRGVPIGPNDYIVA
                     ASAVAHRATLVTHNTGEFSRVPGLVICDWCEA"
     gene            complement(2340..2561)
                     /locus_tag="PKJ16_09875"
     CDS             complement(2340..2561)
                     /locus_tag="PKJ16_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component"
                     /protein_id="HNV47338.1"
                     /translation="MPQITIYVSKELEKTIVSRAKKEKVSLSRWISARLERSLEYEWP
                     ADYFGLFGSVADIERPPQPDGTRDRRESL"
     gene            complement(2667..3920)
                     /locus_tag="PKJ16_09880"
     CDS             complement(2667..3920)
                     /locus_tag="PKJ16_09880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HNV47339.1"
                     /translation="MATEKNLPFTKQQIEAIIERHPTPFHIYDERAIVDNARRLIRAF
                     AWAPGYTEYFAVKATPNPHLLKLLAGEGFGADCSSLPELVLAERAGVTGERIMFTSND
                     TPAHEFVKARELGAIINLDDISHIPFLEAACGIPEVISFRYNPGPLRTGGSIIGHPEE
                     SKYGFTREQLFEGYRLVRDKGARRFGLHSFIISNELNRGFFVETARMLFELAIELKRE
                     MDLRLEFLNLSGGIGIPYRPDQDAVDLEALSAEIKAEYERTIVPAGLHPMKIFMECGR
                     MVTGPYGYLVSRVLHLKNIYKQYVGLDACMANLMRPALYGAYHHITVMGKEGAPRDRV
                     YDVTGSLCENNDKFAIDRALPAIEIGDIVVIHDAGAHGHAMGFNYNGKLRSAELLLRP
                     DGSVKLIRRAETMDDYFATLDVSSL"
     gene            complement(3960..6527)
                     /locus_tag="PKJ16_09885"
     CDS             complement(3960..6527)
                     /locus_tag="PKJ16_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HNV47340.1"
                     /translation="MRRYSGLSTKMTKPGVKPARNLFGGFRLGRVPVPDFIGMAGTLY
                     DRLRFGIGNYRVVIAGVAAGVLIAFIVILVMDFMKVRSLATYQPNTTTKIYDKNGQLV
                     SELFRQKREVVKLDKIPKHLVNAFIAIEDNEFYDHFGINIKGIVRAFFINIFSGRIKQ
                     GGSTITQQLAKVMLTSGERNLYRKAKEACIAIMMEMAYSKDEILELYLNQIFMGHGSY
                     GVESASRFYFDKHVWELSLAESSLLAALPSAPNKLSPIRYPKTAIERHKVVLAKMIEL
                     GFVRIKEAEDAFLAFWPNYLADLSDMSPSMNTWSTRLNRTPWFTEYIRRELVKKYGEK
                     MVYEEGLNVYTTLDITKQLAAQETLRRGLLKQSTVSKGLAFKNYHYVAENYSGLVELF
                     ALMFDIDPFAKKGSVESGRFNAYLESGLLDELEALNLIVGLDVIDIALDGYKRQFTEE
                     VEFQQVEGALISINPTNGYIEALVGGREFSQTNQLNRAMQSRRQPGSSIKPLIYAAAM
                     ESGKFSPGSAVLDSPIIYLDSQHGDWIPENYEGEYYGLIRLRRALAMSINVVTIRVAE
                     TLGLEYVIRYLDKLLKFDEAESKQRIPRNMSIALGSFEVSPFELARAYAIIANGGKDV
                     IPFSIRSVRDRRGTVLENQEEEVRAQLLKEEKNGSLQIIKPETAQVMISMLRSVISEG
                     TGRGASPGRVAGGKTGSTNNWKDAWFVGFVPQLATCIWVGYDKLGLSLGVGQAGGGVA
                     APMWGQYMREALKNEPVLQFPNYAPLAGGRVCASSGLLPSPSCKEVIDEIFVPGSQPD
                     KACDVCSGEAGVKVGSFRRGPKENITSDQKKSIMSNIKKRSGEGRPVLRDFGDDLLD"
     gene            6738..8669
                     /locus_tag="PKJ16_09890"
     CDS             6738..8669
                     /locus_tag="PKJ16_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV47341.1"
                     /translation="MVLQIAVYRRWARAVRLLSAALLFALYAHSIAHGNPVPPAIELS
                     DTHAEYEAAGNMLYREDPTKMLTIDDVTKPGSPWKESPRHTPNFGFSTSAYWFAFRVK
                     NTRPERKWVFSIRYPLFDDVRLYAPRDGTLDLIYHTGDMLPFHNRPILDNTFTVPLHF
                     DDRNEITFFVRISTESAFNCIFSVMEEGRFHDIRAREKLVYGLYFGILLVISLYNLLI
                     FIKVRDKNYIFYIMFVITHGLFQMSFHGLSMEYLWPHSPIWNNIALNFFGGQSIIWTV
                     LFAKYFLETGRTAPRLDKALLFFIIPGGVQMLTSFFLPYSIPARIGTMTLIPFPVLLW
                     LSGFIAYRSGFRQARFYLLAWSVYLAFVVVWALSRFALLPTSIISEYGMQIGSAIEAV
                     LLSLALGDRMNILGEEKILAQRNLIEEQNQSLALQKQYTESISRFVPSEFLSFLHRDS
                     IIHVELGDQTIERMCVLFSDIRSFTAISERLSPRENIDFINAYLSRVGPVIREHHGFI
                     DKYIGDAIMALFPGSPDNALRAALCMKEQVRILSREHILPLPVELRSGIGIHYGPLML
                     GTVGEEHRIDTTVISDAVNVASRLEGLTKKLPADIIISQEVRDALENPAGFRIETIGR
                     VKVKGRERPLLIYSVQDLA"
     gene            8941..9954
                     /locus_tag="PKJ16_09895"
     CDS             8941..9954
                     /locus_tag="PKJ16_09895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV47342.1"
                     /translation="MKRNHTLTGLLVRAMAFAVCVAPLAHCGKKDAANAIAPARVVCL
                     VGSSTIQRNGAAPAGLALGDIVRDGDTLITRASSFIVIQVGDKGVLRIQENTTVAIRG
                     LFTNAASRIILRAGAITSKLVKLDPGSSYSVATNTITASVRGTIFTVSTRGSIDLVSV
                     GEGSVSVAANPGIAVKPFTETIVGAGTTAVIESTAAAAGRGDVTIELRPMGADEVLTT
                     GKVSRIGIAPGIATASADELATIQREILRIESELEKKTGEPGRTDAGPRAEKIRKLMG
                     QSSRTMEQIREAFERIDEVSLYSGRVIRGAIISRGETYAILTPGGIVSAPKADVQAVK
                     VIK"
     gene            9974..10786
                     /locus_tag="PKJ16_09900"
     CDS             9974..10786
                     /locus_tag="PKJ16_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47343.1"
                     /translation="MNTASVRAGITIAIACALITAIAHGSAHAESVFLKNGRILEGKI
                     AAESDRSIDLLLPGGTRTTIPRAEVLRTLYHDNYKQKRYIYKTDGTTIEAHIVDENSE
                     SYTCRTDLNSPVETWIRKSDVETLSKRKIDVSDAGGRPYHSPVVATIASLAPVWSGSW
                     NAGFTWWGLGLCAFKTAGAAGVVYSFIGPTGTGERIGFASLGAWVGFTIIDMIYSYNR
                     IDNHNTRHGLASRILDDSAVGVDLLPRYAADWPLERAHARPDGVSVGFSRRF"
     gene            complement(11009..>12007)
                     /locus_tag="PKJ16_09905"
     CDS             complement(11009..>12007)
                     /locus_tag="PKJ16_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV47344.1"
                     /translation="ALAYNLSVRHGGDRVFLVSKDINLRMKAKSVGVHAEDYETGKIK
                     DIDELYTGKSLVENMSGEFIDRLYREGAVVIDALDRDIAPHPNQYFIMRNGTRSALTT
                     YQKEEKALRIVEKKRAYGITPRNAEQTFSLDALMNPAISLVTLSGKAGTGKTLMALAA
                     ALERRKEYKQILLARPVIPLSNRDIGYLPGDIAEKIDPYMQPLYDNLSVIKNQFSEES
                     PDFKRIAEMVTNGKLHIMALAYIRGRSLSKVYFIVDEAQNLTPHEVKTIITRAGEGTK
                     IVFTGDPHQIDTPYLDSRSNGLTYLIDKMKGQDIYAHVTLEKGERSHLAELASDLL"
BASE COUNT         2275 a         3780 c         3591 g         2361 t
ORIGIN      
        1 tccctcctcg cgtcggtgcg cgtggactgc atcgcgatcg acgaggcgca ctgcatctcc
       61 gaatggggcc acgacttccg cccggagtac cgtcagatgg ccgccttccg ggccggtttc
      121 cccgacgccg tctgcgtggc cctcaccgcg accgcgaccg aggcggtgcg ccgcgacatc
      181 gtggagagcc tgcgcctgcg cgacgcgggc gtgtacgtgg ccagcttcaa ccgcgagaac
      241 ctcttcttcc aggtgctccc caaggacgac gcctacgccc agctcgcggc gttcctgagc
      301 gcgtttcccg ggcagtcggg gatcgtttac tgcttttcgc gcaacggcgt ggacgccctc
      361 gccgcgcgcc tcgccgacgc gggacattcg gtcctcccct atcatgcggg gctcgaggac
      421 gaggtccgca aacagaacca ggagcgcttc atccgcgacg aggtgcagat catcgtcgcc
      481 accatcgcct tcgggatggg gatccacaag tcgaacatcc gcttcgtggt gcactacgat
      541 cttcccaaga gcatcgaatc gtactaccag gagacggggc gcgcgggacg cgacggcctg
      601 cccgcgcact gcctgctcct gtacagctac ggggacatcc acaagatcag gtttttcatc
      661 aaccagaagg aggacgaccg ggagcgcgcg aacgccgagc gccacctcag ggcgctggtc
      721 gattacgccg aggcgaccgg atgccgtcgc gcgcccctca tcgcctattt cggggaggcc
      781 tacgagcacg acgactgcgg catgtgcgac aactgcacgc agccgccggc gccgggcgag
      841 gacctcaccg tccccgcgca gaagctcctc tcctgcgtga agcgcgtgaa cgagcgattc
      901 ggcgtcaacc acatcatcga cgtgctgcgc ggatcggaat cgcacaagat tctctcgtac
      961 gaccatcacc gactgtccac ctacggcatc ggggcggact actcgaagaa gcaatggctg
     1021 catctggcgc gccagttcat ccggcagggt ttaatggtcc aggacatgga aaacttcggg
     1081 gtcctcacga tcacccggct cggctacgac gttatgcgcg gcgacgccac ggtgcgcggc
     1141 acgattatca acgaccggcg cgccgttttc gccggcggga aatccgcaca ggccgactac
     1201 gaccgcgagc tcttcgagag gctccagaaa cttcgcatgg cgcttgcatc agccgggggc
     1261 gtgccaccgt acgtcgtctt ctccgaccgg acgctttccg agatggcctc gcgctatccc
     1321 cgcgaccggg ctgcgctcct ggacatacac ggcgtcggcg agcacaagtg cgagcggtac
     1381 ggcgatgagt ttctggtgat gattcgggac tactgcgcgg aacgaaacat cgactgagcg
     1441 gccgtcggtc acctcatcca aacgcccagt accgcgccgc gcgctggtcg atgatctcga
     1501 aggaaaccga ctcggggacc gaacggttgc gcatgaaacg catggcgatg gagttcgcgt
     1561 agttccccgc gtccgccatc gtctgccact cgtagagccc ctggcacagg ccggtcccct
     1621 cctccaccgc ccagtatttc tggcgaaacc cgcgaaagcc catgaatacc agcatgggaa
     1681 gcagtgaaaa gacgcgattg gcgcgatcgc tcatccgcct tggcttgaag cgcacgagaa
     1741 acaccgcttc gggatcgggg ctgatcccct tcgggcgtat cttcacatga cgaaacaccg
     1801 tgaacaccct gccgtcgggc attgcgagct gcttcccgac gctggagccc agaaagagca
     1861 cgcgcccgct catgatgagc ccgagggtcc ggatgaagcc gatgagggga ttggtgttca
     1921 tgacgggatg ccgctcgctt acgcctcgca ccaatcgcag atgaccagac cgggaacacg
     1981 cgagaactcg ccggtattat gggtaaccaa tgtcgcccga tgggcgaccg ccgacgccgc
     2041 gacaatgtaa tcgttcggtc cgatcggcac tccccgcttc tcgagccctg cgcggatatc
     2101 ggcataacac tgcgcttcac gctccccgaa ggggattcgc gtaatcggtt caagaaacag
     2161 cgacaccgcc ctgatcgtcg cctcccgggc gctgctcttg tacgcgccca gcagcaattc
     2221 cgcttcaacg atggttggga tggcgacatc cgaaggccgt acggatttca gatgcgccgc
     2281 aatcgacgga aacatgttct tcagcgcata gacgcagata ttggtgtcga ggatatattt
     2341 catagcgact cgcgacggtc ccgggttcca tccggttgcg gcggccgttc gatatccgcc
     2401 accgagccaa acagaccaaa atagtccgcg ggccattcat attcgagtga acgctccagg
     2461 cgcgcgctga tccatcggga caaggaaacc ttttcctttt tcgcgcggct cacgatcgtt
     2521 ttttcgagct ccttgctgac atagatggta atctggggca tatggggctc ctgacggcag
     2581 ggatagaaca agtatacgcg gacatatacg cttcgtcaaa caaaaagcgc acgcaaacgt
     2641 cgggtcttta cccggcagga tgctttctac agcgacgaca catccagcgt cgcgaagtag
     2701 tcgtccatcg tctcggcgcg gcggatgagc ttcacagagc cgtccgggcg cagcaggagc
     2761 tccgccgagc gcagcttgcc gttgtagttg aagcccatcg cgtgcccgtg cgcgccggcg
     2821 tcgtggatca ccacgatgtc gccgatctcg atcgccggga gcgcgcggtc gatcgcgaac
     2881 ttgtcgttat tctcgcacag cgagccggtc acgtcgtaca cccggtcgcg cggcgcgccc
     2941 tcctttccca tcacggtgat atgatgatag gcgccataga gcgccgggcg catgaggttc
     3001 gccatgcagg cgtcgagtcc cacgtactgc ttgtagatgt tcttcaggtg gagcacgcgc
     3061 gagaccaggt agccgtacgg gcccgtgacc atgcggccgc actccatgaa tatcttcatc
     3121 gggtgcagcc cggccgggac gatcgtgcgc tcatactcgg ccttgatctc cgcggagagc
     3181 gcctccaaat caaccgcgtc ctgatcgggc cgatacggga tcccaatgcc gccggagagg
     3241 ttcagaaact ccagccgcag gtccatctcg cgcttgagct cgatggcgag ctcgaagagc
     3301 atgcgcgccg tctccacgaa gaagccgcga ttgagctcgt tggagatgat gaagctgtgc
     3361 agcccgaaac gccgcgcccc cttgtcgcgc accaggcgat agccctcgaa gagctgctcg
     3421 cgcgtaaagc cgtacttcga ttcctccgga tgaccgatga tggagcctcc cgtcctgagc
     3481 ggccccgggt tgtagcggaa ggagatcacc tccggaatgc cgcaggcggc ctcgaggaac
     3541 gggatgtggc tgatgtcgtc caggttgatg atcgcgccga gctcgcgcgc cttgacgaac
     3601 tcatgcgccg gcgtatcgtt cgaggtgaac atgatgcgct ccccggtgac gccggcgcgc
     3661 tccgcgagca cgagctccgg cagcgagctg cagtcggcgc cgaagccctc gcccgccaag
     3721 agcttcagga ggtgcgggtt cggcgtcgcc ttcacggcga agtactcggt gtagccgggc
     3781 gcccacgcga aggcgcggat caaccgccgg gcgttatcga caatcgcgcg ctcgtcgtaa
     3841 atatgaaacg gcgtcgggtg ccgctcgatg atcgcttcga tctgctgttt agtgaacgga
     3901 aggttctttt cggttgccat ggtgttgttc tctgttctat gatgctctgt gcgccgcgct
     3961 caatcgagca ggtcgtcgcc gaagtcccgg aggaccggcc tgccctcccc cgagcgcttc
     4021 ttgatattgc tcatgatgga cttcttctgg tcggaggtga tgttctcctt gggcccgcgg
     4081 cgaaaggagc ccaccttcac gccggcctcg cccgagcaca cgtcgcatgc cttgtccggc
     4141 tgcgaacccg gcacgaatat ctcgtcgatc acctccttgc aggaaggcga cggcagcagt
     4201 cccgagctgg cgcagacccg cccgccggcc agcggcgcgt aattggggaa ctgcagcacc
     4261 ggctcgttct tgagggcctc gcgcatgtac tgtccccaca tcggcgcggc gaccccgccc
     4321 cccgcctgtc ccacgcccag cgacaggccc agcttgtcgt atccgaccca gatgcacgtg
     4381 gccaactgcg ggacaaagcc cacgaaccag gcgtccttcc agttgttggt gctcccggtt
     4441 tttccgccgg cgacgcggcc ggggctggcg ccgcgccccg tgccctccga gatgaccgat
     4501 cgcagcatgc ttatcatcac ctgcgccgtt tcgggtttga tgatctggag ggacccgttc
     4561 ttttcctctt tgaggagctg cgcgcgcacc tcctcctcct ggttctcgag caccgtcccc
     4621 cgccggtcgc gcaccgagcg gatactgaag gggatcacgt ccttcccgcc gtttgcgatg
     4681 atggcgtagg cgcgcgcgag ctcgaagggc gacacctcga aggagcccag cgcgatggac
     4741 atgttgcggg ggatgcgctg cttcgactcg gcctcgtcga acttgaggag cttgtccaga
     4801 tagcggatca cgtattccag gcccagcgtt tcggcgacgc ggatggtgac cacgttgatg
     4861 gacatcgcca acgcccgtcg caagcggatg aggccgtagt actcgccctc gtagttctcg
     4921 gggatccagt cgccgtgctg gctgtccagg tagatgatgg gcgagtcgag cacggcgctc
     4981 cccggcgaga acttgcccga ctccatggcc gccgcgtaga tgagcggctt gatggaggag
     5041 ccgggctgcc gccgcgactg catggcgcgg ttgagctggt tggtttgcga gaactcccgg
     5101 ccgcccacca gcgcctcgat gtagccgttg gtcgggttga tggagatgag cgcgccctcc
     5161 acctgctgga actcgacctc ctcggtgaac tgccgcttgt agccgtccag cgcgatgtcg
     5221 atgacgtcca aaccgacgat caggttgagc gcctccaact cgtcgagcag gccgctctcg
     5281 agataggcat tgaagcgacc ggactcgacc gaccccttct tcgcgaacgg atcgatgtcg
     5341 aacatgagcg cgaagagctc caccagcccc gagtagttct cggcgacgta atgatagttc
     5401 ttgaaggcca ggcccttcga caccgtggac tgcttcagga gcccgcggcg cagcgtctcc
     5461 tgcgcggcga gctgcttagt gatgtcgagc gtcgtgtaca cattgagccc ctcctcgtac
     5521 accatcttct cgccgtactt cttgacgagc tcgcgcctga tgtactcggt gaaccacggc
     5581 gtgcggttga gccgcgtgga ccaggtgttc atggacggcg acatgtccga aaggtccgcg
     5641 aggtaattcg gccagaaggc gaggaaggcg tcctcggcct ccttgatgcg cacgaagccc
     5701 agctcgatca tcttggccag caccaccttg tggcgctcga tcgcggtctt gggatagcgt
     5761 atgggcgaga gcttgttggg cgccgagggc agcgccgcca gcagcgagga ctccgcgagc
     5821 gagagctccc acacatgctt gtcgaagtaa aagcgcgacg ccgactccac gccgtaggag
     5881 ccgtgcccca taaagatctg gttgaggtac aactccagga tctcgtcctt cgagtaggcc
     5941 atctccatca tgatggcgat gcaggcctcc ttcgctttcc ggtagaggtt ccgctcgccg
     6001 gaggtgagca tcaccttcgc gagctgctgg gtgatggtgc ttccgccctg cttgatccgc
     6061 cccgaaaaga tgttgatgaa gaaggcccgc acgatcccct tgatgttgat gccgaagtga
     6121 tcgtagaact cattgtcctc gatcgcgatg aacgcgttca ccaggtgttt cgggatcttg
     6181 tcgagcttca ccacctcgcg cttctggcgg aagagctcgg agacgagctg tccgttcttg
     6241 tcgtagattt tagtggtggt gttgggctga taggtcgcga gcgaccgcac cttcatgaag
     6301 tccatgacga ggatcacgat gaacgcgatg agcacgcccg ccgcgacgcc ggcgatcacc
     6361 acgcgatagt tgccgatccc gaagcgcagc cggtcgtaga gcgtgcccgc catgccgatg
     6421 aagtcgggaa cgggaacgcg cccgagccga aagcccccga agaggttccg ggcgggcttc
     6481 acccccggct tcgtcatctt cgtggacagc ccgctatacc tgcgcatgcc gccccgctcg
     6541 tttttccgca cactgtgcca tggtcggcaa cagatagaag cgaatcgttt atttcaagta
     6601 gaaatttaac gattaaaaca ggcctcccgt acacttttac taaacggttc gctggtcttt
     6661 tgaaaactga ttgacgcccc atcttttttc atgatcatac tgtgacagcg aacatgactc
     6721 cgatgccacc aattgatatg gtcttacaga tcgccgtata ccgtcgatgg gcgcgtgccg
     6781 tacgactcct ttccgccgca ctgctgttcg cgctctacgc acactccatc gcgcatggaa
     6841 atcccgtacc tccggccatt gaactttccg atacgcacgc ggaatacgag gcggccggga
     6901 acatgctgta tcgtgaagac ccgacgaaaa tgctcaccat agacgatgtt acgaaaccag
     6961 ggagcccgtg gaaggaatcc ccacggcata cgcccaattt cggcttttcc acatcggcct
     7021 attggttcgc ctttcgcgtg aaaaacacac ggcccgagcg gaaatgggta ttttccatcc
     7081 gctatcccct cttcgacgac gtgcgactgt atgcgccacg ggacggaaca cttgacctga
     7141 tataccacac cggcgacatg ctccccttcc acaaccgtcc cattttggac aacaccttca
     7201 ccgttcccct ccacttcgac gacaggaatg aaatcacttt tttcgtgagg atttccacgg
     7261 aaagcgcgtt caactgtatt ttttccgtca tggaagaagg gcgtttccac gacatccgcg
     7321 cgcgggaaaa attagtatac ggtctgtact tcggcatcct cctggtcatc agcctgtaca
     7381 acctcctcat attcatcaag gtgcgcgaca agaattacat cttctacatc atgttcgtca
     7441 tcacgcacgg attgttccag atgtcgtttc atgggctttc catggaatac ctctggccgc
     7501 acagcccgat ttggaacaac atcgcactta atttcttcgg cggccagtcc atcatctgga
     7561 ccgttctgtt cgcaaaatat ttcctcgaga cgggccgcac cgcgccccga cttgacaagg
     7621 ccctgctctt tttcatcata cccggaggag tacagatgct cacgtctttc ttcctcccct
     7681 attccatccc ggcgcggatc gggacaatga ccctcatccc ctttcccgtg ttattgtggc
     7741 tgtccggttt tatcgcctac cgatcgggct ttcggcaggc ccgcttctat ctgctggcct
     7801 ggtcggtcta cctggcgttc gtcgtggtct gggccctgtc gcgcttcgcc ctgctgccga
     7861 ccagcatcat cagcgaatac gggatgcaga tcggatcggc catcgaggcc gtgttgctct
     7921 cgctggcgct gggagaccgg atgaacattc tgggggagga gaagatactg gcacagcgca
     7981 acctcatcga ggagcagaac cagagcctgg cgctgcaaaa acagtatacc gaatccatct
     8041 cgcgtttcgt tcccagcgaa tttctcagct ttctccaccg ggacagcatt atacacgtcg
     8101 aactggggga ccagacgatc gagcggatgt gcgtgttgtt ttctgacatt cgctccttta
     8161 cggcgatctc ggagcgactg tcgcccaggg agaatatcga tttcattaac gcctatctgt
     8221 cccgcgtggg ccccgtcatc cgggagcacc acggcttcat cgacaagtac atcggcgacg
     8281 ccatcatggc gctctttccg ggaagcccgg acaatgccct gcgggcagcc ctctgcatga
     8341 aggaacaggt tcggatcctc tcgcgcgaac acatcctccc gctgccggtg gagctccgat
     8401 ccgggatcgg cattcactac gggccgctga tgctgggcac cgtgggcgag gagcaccgca
     8461 tcgataccac cgtcatctcc gacgccgtga acgtcgcctc ccgcctggaa ggccttacga
     8521 agaagctgcc ggccgatatc atcatcagcc aggaggtgcg cgacgccctg gaaaacccgg
     8581 ccggttttcg catcgagacg atcggaaggg tcaaggttaa gggaagggaa cgccccctgc
     8641 tcatttattc ggtccaggac ctcgcatgag tacgctgtac agcgttgcat ggaacacctc
     8701 gtatccttac ctacttgtcc agcgcagaaa tccgttgagt gatgtacaat cgaaaagccc
     8761 gctatcgcct tcattcccgt aatcatgtcg ttcattcccc ataccgtctt tcgacattga
     8821 cacgcaacca cccgcagacc tgatcgttct tttcgggaac gcaccggcgc cacggcacgt
     8881 atgcatatcg aaataggccg ggaatgagga aattattttt tcatacggag cgtgtatcac
     8941 atgaaaagaa accatacgct gacgggactg ctggtgcgcg caatggcgtt tgccgtctgc
     9001 gttgcgccgc ttgcgcactg cggaaaaaaa gacgccgcga acgcgatcgc cccggcgcgg
     9061 gtcgtctgtc tcgtcggctc ctcaacaatt caacgaaacg gcgccgcgcc ggcggggctc
     9121 gccctgggcg acatcgtccg cgacggggat acgctcatca cgcgcgcctc atcgttcatc
     9181 gtcatccagg tcggcgacaa aggcgtcctt cggatccagg agaacacgac ggttgcgatc
     9241 cggggtttat tcaccaacgc cgcgtcgcgg atcatccttc gcgcgggcgc gatcacctcg
     9301 aagcttgtga aactcgaccc gggctcgtcg tattccgtcg cgacgaacac gatcaccgca
     9361 agcgtacgcg gaacgatatt caccgtctcg acgcggggat cgatcgatct cgtctccgtc
     9421 ggagaagggt cggtatcggt ggcggccaat ccgggcatcg cggtcaaacc gttcacggaa
     9481 acgatcgtcg gcgcgggcac caccgccgtc atagaatcca cggcggccgc agcggggcgc
     9541 ggggatgtca cgatcgagct acgcccgatg ggcgcggacg aagttctcac aacgggaaaa
     9601 gtatcccgga tcgggattgc gccgggcatc gcgacggcat cggccgatga gcttgcaaca
     9661 attcagcgcg agatccttcg catcgagtcg gagctcgaga aaaagaccgg ggagcccgga
     9721 cgcacggacg cgggacccag ggcggagaag atcaggaagc tgatggggca gtcgtccaga
     9781 acgatggagc agatcaggga ggcgttcgag cgcattgacg aggtctcgct gtattccggg
     9841 cgcgtcatcc ggggagcgat catcagccgc ggcgaaacct atgcgatcct tacgccgggc
     9901 gggatcgttt ccgcgccgaa ggccgatgtg caggcggtga aggtaatcaa gtaagcaggg
     9961 tggtcaatcg aatatgaaca cggcatcagt acgagcaggg atcacgatcg cgatcgcatg
    10021 cgcgctcatc acggcgatcg cgcacggctc cgcgcatgcg gaatcggtct ttctaaaaaa
    10081 cgggaggatt ctggaaggga agatcgccgc ggaatcggac cggagcattg acctgctcct
    10141 gcccggcgga acgcgcacga ccatcccccg ggccgaggtc ctgcggacgc tctatcatga
    10201 caattacaaa cagaagcggt acatctacaa gaccgatggc acgacgatcg aggcgcatat
    10261 cgtcgacgag aacagcgaat cctacacctg ccgaacggac ctgaattccc ccgtggagac
    10321 ctggatacgc aagagcgacg ttgaaacgct ctcgaagagg aaaatcgacg tttcggacgc
    10381 cggtgggcgg ccgtatcatt ctcccgtcgt tgcgacgatc gcctcgctcg cgccggtctg
    10441 gagcggcagc tggaatgcgg gcttcacctg gtggggcctg ggcttatgcg cgtttaaaac
    10501 ggccggcgcc gccggcgtcg tgtattcgtt catcggaccg accggcacgg gcgaacgcat
    10561 cggctttgcg tcgctgggcg cctgggtcgg gtttacgatc atcgacatga tctattcgta
    10621 caaccggatc gacaatcaca acactcggca cggtctcgcg tcacggatcc tcgacgattc
    10681 ggcggtcggc gtcgacctgc tcccgcgata tgccgccgac tggccgctgg agcgcgcaca
    10741 cgcccggccg gacggcgtga gcgtcggatt ctcccggcgg ttctgactgg aacgccatag
    10801 cacgcacgga cgcaatcgca gtccgatatg aggattcatg cacatcaatc cgtgcgttcc
    10861 gtcatcttct tttcgatgaa tttcacatcg agcatgtcac agtcgcgttt ttactcgtca
    10921 caggaggcca cggccttctc tctcatgacc ttgagatgct cacgtgaaga gacagaggaa
    10981 aaactttttc actgatgacg gattaatcct acaacaggtc cgacgccagc tccgccaggt
    11041 gcgagcgctc gcccttctcg agcgtcacgt gcgcgtagat gtcctgcccc ttcatcttgt
    11101 cgatgagata ggtgagcccg ttggagcgcg agtcgaggta gggcgtgtcg atctggtgcg
    11161 ggtcgccggt gaagacgatc ttggtcccct ccccggcgcg cgtgatgatc gtcttcacct
    11221 cgtgcggcgt gaggttctgc gcctcgtcca cgatgaagta caccttcgag aggctgcgtc
    11281 cgcggatgta agcgagcgcc atgatgtgca gcttgccgtt ggtcaccatc tcggcgatgc
    11341 gcttgaagtc gggcgattcc tcgctgaact ggtttttgat caccgagaga ttgtcgtaga
    11401 gcggctgcat gtacggatcg atcttctccg cgatgtcgcc ggggaggtag ccgatgtcgc
    11461 ggttcgagag cgggatcacc gggcgcgcga ggaggatctg cttgtactcc ttccgccgct
    11521 ccagcgccgc cgcgagcgcc atgagcgtct tccccgtgcc cgccttgccg gagagcgtca
    11581 ccaggctgat cgccgggttc atgagcgcgt ccagcgagaa ggtctgctcg gcgttgcgcg
    11641 gcgtgatgcc gtaggcgcgt tttttctcca cgatgcgcag ggccttctct tctttttgat
    11701 acgtcgtgag ggcgctgcgc gtgccgttgc gcatgatgaa gtactggttc gggtgcgggg
    11761 cgatatcgcg gtcgagcgcg tcgatcacca cggcgccctc gcggtacagc cggtcgatga
    11821 actcccccga catgttctcg acgaggctct tgccggtgta gagctcgtcg atgtccttga
    11881 tctttccggt ctcgtagtcc tcggcgtgga cgcccacgct cttggccttc atgcgcagat
    11941 tgatgtcctt ggagaccagg aagacgcgat cgccgccatg gcgcaccgac aggttgtagg
    12001 ccagcgc
//
LOCUS       DAORHF010000103        11915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4504_length_11915_cov_4.731956, whole genome shotgun sequence.
ACCESSION   DAORHF010000103 DAORHF010000000
VERSION     DAORHF010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4504_length_11915_cov_4.731956"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2231
                     /locus_tag="PKJ16_09910"
     CDS             <1..2231
                     /locus_tag="PKJ16_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV47345.1"
                     /translation="LFVSILHQINRQTPVAFKCSFRDKITFYTRIFPFWATRSLMGRV
                     NKKISDLIRTVARSSEMSTGFNNNMAAIAHFIVNFDRVLELGTTGLIEEIRTIQREKP
                     GNDQDFYRGAIIALEALAAFADRYADKLMEASILEADPARREELKAMAEICRHVPRNP
                     ARTYHEALQSMLFLHIALCIESYENAVSFGRLDQILHPYYQRDLKAGIITYDRAKELL
                     ALFIVKMDEVVLVNDGDTYLGLGRLFETMSTDQTVTAGGMGKDGKDATNDLTYMLLDI
                     CELQPYAVNMTARIHRNSPQEYLDRLAEIYINGAPMPALYNDELYIDTLTRHYDTSIR
                     DARNYSIVGCVEPNASDEHFGNTDCANMNLALPFLQALKGHENDLWNIGVIDQLERMH
                     TKFIEYNFKGKNPVSRLIRSVYAWARNRFIAMRTPAYKSPRNMDELLDRFQKRLNHLA
                     QSILADHQKIEGVLREGFTTPLASSLFKSCVEQGKDAYHGGCRFNSSGIQGIAITDVA
                     DSLHAIDEVVYKKRRYSIDEVIEAIDANFEGEKNREIQSALLAVPKFGQDESPESVAW
                     VNKTLQIYVNALNSVPNCPRDGIYTAGYYALNVNGVYGRKTPALPSGRLAGTPLANSV
                     SPHHGMEHGDLLSSLNSVAGADFASYAPNGTTVTFTIDAALFQGAEGVRNLASIFSTF
                     FKKGGMQFQPNVLNREILLDAYNNPEKYPFLLVRIAGYCAYFTQLSDELKKEIINRTC
                     YS"
     gene            complement(2333..2653)
                     /locus_tag="PKJ16_09915"
     CDS             complement(2333..2653)
                     /locus_tag="PKJ16_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47346.1"
                     /translation="MYHRTRNFILVIGGIVIFFAADVALAVMLAMRDARTAVDFVFKV
                     FGASMAFLFAAIGPLGLIGLALMSGNMRGVKLVAGLACAALASILLLVYVMNEFDVFS
                     LFAH"
     gene            complement(2751..3251)
                     /locus_tag="PKJ16_09920"
     CDS             complement(2751..3251)
                     /locus_tag="PKJ16_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47347.1"
                     /translation="MKRLCVLCVSILLVAASGVVSYENATTFKEMVDMRVKKRWKTPI
                     SHPMMYRCIPKRWRTNRKFEAEIMGALVRKFPEFNTGDVVRVLFDTDHWNVSRDEFTG
                     FIRNRYLFAHLIVPAERSGEYYIVWLDFYQRNRFLGYLYHEPQFRKLLGVDRIKKEHL
                     DVTCTY"
     gene            3434..4654
                     /locus_tag="PKJ16_09925"
     CDS             3434..4654
                     /locus_tag="PKJ16_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV47348.1"
                     /translation="MKHPHGIILASLVLLHGCAFSLLSGNFDRDVRAIETLSREAQAL
                     VDSAYRDIPPGKLVDCHLHVVGINTAKTSCFVNENMPKWWHPRAYIRYRLFLSAFGVD
                     ATDTTDEAMVRRLASLARGMRGGGTFYILAMDKNYNRDGTENLPQTEFYTPNNYVYEI
                     SRAHPDIFVPVVSINPYRKDAIAELERWAGKGVRMVKWIPNAQGIDPSDPAIDPFYRR
                     MKALRMALLTHGGEEKAVDAEEAQKLGNPLLLRRPLDIGVTIIVAHGASLGRNIDLDD
                     PEKKPADNFDLFLRLMDDKKYERLLFADVSAQLQFNRLSKPLERILERTDLHHRLVNG
                     SDYPLPAVNVVIQTRVLARLGFITADERRCLNEIYRANPLLFDFVLKRTIRHPRTGAR
                     FPASVFTTGAVPIR"
     gene            complement(4676..5533)
                     /locus_tag="PKJ16_09930"
     CDS             complement(4676..5533)
                     /locus_tag="PKJ16_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47349.1"
                     /translation="MTGRMLLVAVLFLSATLPAVAGSSFSFTIDACTVKKAGAGYGVD
                     LRIAYRGPATIFSGASVPITTERIVIAGNAHGKILERDFLMSRFAYDINGNGNATDEF
                     PAAIRNDTLVIGPTVLDPVLDRRTVNGMQLLSYFDRQGRARLYRIGQAGRPFVVYQLA
                     GSNAVLGITTQEAPVSLEKFPNPNVQVMVVKMQERRFERPSYAIVGDRNFLTFSNERF
                     FPDQTDEWVSIVWAVRDIDVSGGGTRFPVSFALSGIKPPFAVIAAVNCSVDDGVRLRT
                     FPTLRMVDH"
     gene            complement(5678..7075)
                     /locus_tag="PKJ16_09935"
     CDS             complement(5678..7075)
                     /locus_tag="PKJ16_09935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV47350.1"
                     /translation="MKTKRFDFDVAIAGAGPGGTLAALRLAEAGLSVGLFDALPEVGL
                     GRSLVLETEPTVYSEVGLPLPSGDTVPYECARLRVLSARGRELFSFDEREHALPVAIN
                     LDVFVKGLLVQAKRAGARFVPGTRVISAITSGERVTGFICKGSDGRDRHVNARITIDA
                     TGFAAALVRSLPASFGFEFPESSRHVVSAANCLHRLVPAKADAAIGNGLFAPDEIIAR
                     MGTTGSYSTVYSFLSKRDGTAYLLVGLKRDHERIITARQVVDAFVRDAGCFGTQVRAA
                     AAPIRIRHSLDRMVAGGFMALGEAACTVFPMHGSGVASALITATLASGVAVNAIRGND
                     VSAAALWSYAAAYQRGRGRRLAGYDVTRLTVDSLSADDVASMLESGIMQAEDTLNGLQ
                     VNEPRISARTVPARIRGFSAHPTLLPPIARMGMTLRRVLDHYARYPERDDPVAFTAWC
                     AKKKRIFGRLLEERS"
     gene            7233..7763
                     /locus_tag="PKJ16_09940"
     CDS             7233..7763
                     /locus_tag="PKJ16_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47351.1"
                     /translation="MQPIRIQATPPRDIAAYRDIVKRGLGPYFFFGWFSITGPAIPTA
                     MMLAHAIIGDAPEEIFVPGVIAFVVAGAILGTILLRSGSNIKRERELALSRGVMIPAT
                     VLAHGRAFAFYKSVRDYTLTVEYAGPDGAPHRSIIRSSNRELHRHDPGDTIMGLRDET
                     SGAVFFPAEAGCELHA"
     gene            complement(7797..8222)
                     /locus_tag="PKJ16_09945"
     CDS             complement(7797..8222)
                     /locus_tag="PKJ16_09945"
                     /inference="COORDINATES: protein motif:HMM:NF024426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HNV47352.1"
                     /translation="MKHQVVAVLIILLLAAGCGKRSITGAEREAVLGYANPASDRILA
                     AYNDGTYARYVTDFDAQMKNAMPENVFVQTRELIMSKIGKYLSREVSDVSQKDQFVVV
                     KYKGKFEREDGVEIKVVFQKIGDRDLVSGLWFNSPKLRQ"
     gene            complement(8481..9101)
                     /locus_tag="PKJ16_09950"
     CDS             complement(8481..9101)
                     /locus_tag="PKJ16_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNV47353.1"
                     /translation="MIELDTIIVFFQTYGYAAVFGMLLLCGLGLPVPEDISIVAGGVL
                     SGLGFTDIRVMGVGVMASVLIGDGIIYFAGRYLGMKALEYRFTRALVNGPRFHAIQGW
                     FGKYGRWVIFAGRFMPGFRSPIFLTTGILRVVPPGAFFAIDGFAALISVPVWLAVGYF
                     GAHNRERLMEWMTQGHVAVGVLVGVIIIAIAIKMIIRRRLEADSAS"
     gene            9272..10465
                     /locus_tag="PKJ16_09955"
     CDS             9272..10465
                     /locus_tag="PKJ16_09955"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HNV47354.1"
                     /translation="MRPHRHVRCMIAALAAFTIVSCRLEVPVREMAQAKSTITRAHEV
                     KAHKYAPDELKKATDHLMSSHRFAAAGDKGAATDTALKSLSFANTAVAKSLPLLSKDS
                     LDAATAKVNEAASLADPAAKPSELVDAEQKLARAGQLHSESKFWESYLISKDIAPALD
                     GFIGKQSEDLKQSAAQRLEAAQNALRTCRAAGPDDSQKADIDKAAALIDKAGNALSSG
                     AYKDSIAASDEAISILNAATIAMEKKREATIIDTKKEKELTEKTDTDRRTYKVVYNPK
                     KRDCLWRIALKMYKNPRLWPLIYVANKDKIKDPDLIYPGQVFIIPPVPSKKPSSDQKT
                     DGESDDDDEDDDTESVDETNATDKSTDSADKKPSDKTETPSPKKNAPADATDAPEDYS
                     DEDAP"
     gene            10559..11011
                     /locus_tag="PKJ16_09960"
     CDS             10559..11011
                     /locus_tag="PKJ16_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47355.1"
                     /translation="MIHTIATLTIALVVAATAQANTLGAKQDTRRIADEFMKKIQTGD
                     FDAAFGGIKIFFPIPEDEFNNLVTQTKNQFQMVKNRFGTTIGYELFKEESIKDLMVKY
                     TYIMKFEKHIIRWMFIFYKPTQGWLLNWFHWDDKIDALFNEPLENNKK"
     gene            11058..11288
                     /locus_tag="PKJ16_09965"
     CDS             11058..11288
                     /locus_tag="PKJ16_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47356.1"
                     /translation="MGAQSYNTTKSSNKPRCKLPELCTSSYLIGNTVFALRDAGRERD
                     VAEFLSRLKPYEDEINFSIVLEVAVDYVEFYD"
     gene            complement(11322..>11915)
                     /locus_tag="PKJ16_09970"
     CDS             complement(11322..>11915)
                     /locus_tag="PKJ16_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47357.1"
                     /translation="LHDILSGADTERVREMMATVLGDVLKGKVQVAVRAETMRSLVAG
                     YLFDLNQAGGVDGKDPDRMPEATIIPVLPVLSPARGRPLTSLVPGDQVQVRVDKSNTV
                     SMRLARSLNLIEGDTIKPMSAHVHSIKKTKDGYNLYVNIAKGLIGKCEVEADIRIKAG
                     DPFVEQEIKKSRMSLIIGIVAGIVVIVIGVIVILALT"
BASE COUNT         2689 a         3790 c         3309 g         2127 t
ORIGIN      
        1 cccttttcgt gtctatccta caccagatca accgccagac gccggtcgcc ttcaaatgct
       61 cgttccggga caagatcacg ttctacaccc gcatctttcc cttctgggcc acacgcagcc
      121 tgatgggccg ggtgaacaag aagatctccg acctgatccg gaccgtcgcg cgaagctccg
      181 agatgagcac cggctttaac aacaacatgg cggcgatcgc gcacttcatc gtgaacttcg
      241 accgcgtcct cgagctgggc acgaccggtc tcatcgagga gatccggacg atacagcgcg
      301 agaagcccgg caacgaccag gacttctatc gcggcgccat catcgccctc gaggcgctgg
      361 cggcattcgc cgaccggtac gcggataaac tcatggaagc gagcatcctt gaggccgatc
      421 ctgcgcgtcg cgaggagctg aaggcgatgg ccgagatctg ccgccatgtc ccccggaacc
      481 ccgcgcgcac ctaccacgag gcgctccaga gcatgctctt cctgcacatt gcgctctgca
      541 tcgaatccta tgaaaacgcg gtctccttcg ggcggctgga ccagatcctg cacccgtatt
      601 accagcgcga cctgaaggcc ggcatcatca cctatgaccg ggcgaaagag ctgctggcgc
      661 tgttcatcgt caagatggac gaggtggtgc tggtgaacga cggcgacact tatctgggac
      721 tcggacgcct cttcgaaacg atgtccacgg accagacggt cacggcgggc ggcatgggta
      781 aagacggaaa ggacgcgacc aacgacctta cctacatgct gctggacatc tgcgagctgc
      841 agccgtacgc cgtgaacatg acggcgcgca tccatcggaa cagcccgcag gaatacctgg
      901 accgcctcgc ggagatctac atcaacggcg cgccgatgcc ggcgctgtac aatgacgagc
      961 tctatatcga cacgctcacc cggcattacg acacgtcgat acgggacgcg cgcaactact
     1021 cgatcgtcgg ctgcgtggag ccgaacgcgt cggacgagca cttcggcaac acggactgcg
     1081 cgaacatgaa cctggcgctg cccttcctgc aggcgctcaa gggacacgag aacgatctgt
     1141 ggaacatcgg cgtcatcgac cagctcgagc ggatgcacac caagttcatc gagtacaact
     1201 tcaagggaaa aaatcccgta tcccgtctca tccggtcggt ctatgcctgg gcgcgcaatc
     1261 gcttcatcgc catgcgcacg ccggcataca aatcgccgcg gaacatggac gagctcctgg
     1321 atcgcttcca gaagcgtctc aaccacctgg cgcagtcgat cctcgccgac caccagaaga
     1381 tcgagggcgt gctgcgcgag ggcttcacca cgccgctcgc ctcctcgctc ttcaagagct
     1441 gcgtcgagca ggggaaggac gcctatcacg gcgggtgccg cttcaacagc agcggcattc
     1501 agggcatcgc gatcacggac gtggccgact cgctccacgc gatcgacgag gtcgtgtaca
     1561 agaaacgccg gtactcgatc gacgaggtta tcgaagccat cgacgccaat ttcgagggag
     1621 agaagaaccg tgaaatccag tcggcgctcc tcgccgtccc gaagttcggc caggacgaat
     1681 cgcccgagtc cgtggcgtgg gtgaacaaga ccctgcagat atacgtgaat gcgctcaact
     1741 ccgttccgaa ctgtccccgc gacggcatct acaccgccgg atactatgct ctgaatgtaa
     1801 acggcgtgta cggacggaaa acgccggccc tcccctcggg acgactcgcc ggcacgccgc
     1861 ttgcgaacag cgtgtcgccg catcacggca tggagcatgg agaccttctc tcgtcgctca
     1921 attccgtggc cggcgccgac ttcgcgagct acgccccgaa cgggaccacg gtcaccttca
     1981 ccatcgacgc ggcgctgttc cagggcgccg agggcgtgcg gaacctcgcg tcaatcttca
     2041 gcaccttctt caagaagggc gggatgcagt tccagcccaa tgtgctcaac cgcgagatcc
     2101 tgctggacgc ctacaacaac cccgagaagt atccgttcct gctggtccgc atcgccggct
     2161 actgcgccta cttcacgcag ctatcggacg agctcaagaa ggagatcatc aaccggacgt
     2221 gctattccta gcatatcttg tgacctgtac ctcatgtacc atcccttcgg gttttcccgg
     2281 ggggattttt tttgtgatag gctcgcgctg atacgtcgta taggatacgg ggctagtgcg
     2341 caaagagcga gaacacgtcg aactcgttca tcacatagac gagcagtaag atggaagcaa
     2401 gcgccgcaca ggcaagtccc gcgaccagct tcacgccccg catgttcccc gacatcagcg
     2461 ccaggccgat gagcccgagg ggaccgatcg ccgcgaacag gaaggccatg ctcgcgccga
     2521 agaccttaaa gacgaagtcg acggcagtgc gggcatcgcg catcgcgagc attaccgcga
     2581 gtgcgacatc cgccgcgaaa aagatgacga tgccgccgat gacgaggatg aagttgcgtg
     2641 tgcgatggta catgccgtct tttctccgcc cctggtcatt ttttcagaaa gcgcagcggc
     2701 catcaagatc gacatcatca ccccataatg agcgggcgcg cgaagcgcct ctagtaggtg
     2761 cacgtcacgt cgagatgctc ctttttaatc ctgtccaccc cgagcaattt tctgaactgc
     2821 ggctcgtgat acaggtaacc gaggaaccgg ttgcgctggt agaaatcaag ccagacgata
     2881 tagtattcac cggagcgctc tgcgggaacg atgagatgcg cgaacagata gcgatttcgt
     2941 ataaatccgg taaactcgtc gcgcgatacg ttccagtggt cagtatcgaa caggacccgg
     3001 acaacatcgc cggtattgaa ctcggggaat ttcctgacga gtgcgcccat gatctcggct
     3061 tcgaactttc ggtttgtccg ccagcgcttg ggaatacacc gatacatcat aggatgggag
     3121 atgggcgtct tccagcgttt tttcacccgc atgtcgacca tctctttgaa tgtcgtggcg
     3181 ttttcatatg aaacaacgcc agatgccgcc acgagcagaa tcgacacgca caatacgcac
     3241 agcctcttca tgtgacgccc cgttgcaatg aacacctcgt cttaacataa gtgttcccac
     3301 ccactgcctg taaatgcaac aaaaaaacat gcgcccgtga gaggcttgac attaatacga
     3361 tcccatgaca tggtattttt attcgattcg catcgctgac aacaggagca cggacagcga
     3421 ggaggcgcac aacatgaaac atccgcacgg aatcattctc gcatcgctcg tgctactgca
     3481 tggctgcgcc ttctctcttc tgagcgggaa cttcgaccgg gacgttcgcg cgatcgaaac
     3541 gctgagccgg gaggcgcagg cgctcgtcga tagcgcatac cgcgacatcc cgccggggaa
     3601 gctggtagat tgccacctgc acgtcgtggg aatcaacacc gcgaaaacca gctgcttcgt
     3661 gaacgagaac atgccgaagt ggtggcaccc gcgcgcctat atcagatatc gcctgttcct
     3721 gagcgcattc ggcgtcgatg ccaccgacac caccgacgag gccatggtcc ggcgcctcgc
     3781 gagcctcgca cgcgggatgc ggggcggcgg aaccttctat atcctggcaa tggataagaa
     3841 ctacaaccgc gacgggaccg agaatctccc gcagaccgag ttctatacgc ccaataacta
     3901 cgtgtacgaa atctcccgcg cacatccgga catcttcgtg ccggtcgttt ccatcaatcc
     3961 ctaccggaag gacgcgatcg cggagctcga gcggtgggcc gggaaaggag ttcgcatggt
     4021 caagtggatc ccgaatgccc aggggatcga tccgtctgat cccgccatcg atcccttcta
     4081 ccggaggatg aaggcgctcc ggatggcgct cctcacccat ggcggcgagg agaaggcggt
     4141 ggacgccgag gaggcgcaga agctcgggaa cccgctcctg cttcgccggc cgctcgatat
     4201 cggcgttacg attatcgtgg cgcacggcgc gagcctcggg cgaaacatcg acctcgacga
     4261 tcccgagaaa aaaccggcgg acaatttcga tctcttcctg cggctcatgg acgataaaaa
     4321 atacgagcgg ctactcttcg cggacgtatc ggcgcagttg caattcaacc ggctgtcgaa
     4381 accgctcgaa cgcattctgg agcggaccga cctgcaccat cggctcgtga acgggagcga
     4441 ctatcccctg cccgccgtga acgtcgtgat acaaacgcgg gtcctcgcga ggctcggatt
     4501 catcaccgcc gatgagcgcc gctgcctcaa tgaaatctac cgagcgaacc cgctcctgtt
     4561 cgatttcgtc ctcaagcgga cgatccggca tccgcggacc ggcgcacgat tccccgcgtc
     4621 cgtgttcacg accggcgcgg tccccattcg ctgaccgccg tcggtccgga atccgctagt
     4681 gatcgaccat ccgcagcgtg ggaaatgtcc gcagtcggac cccgtcgtca acggagcagt
     4741 tgaccgcggc gatcaccgca aacggcggct tgattccgct cagcgcgaat gacacgggga
     4801 agcgcgtccc gccgccgctc acgtcgatgt cgcggacggc ccacacgatg ctcacccatt
     4861 catccgtctg atcggggaaa aatcgctcat tggagaaggt cagaaaattc ctgtcgccga
     4921 cgattgcata cgacggccgc tcaaaacgtc gctcctgcat ttttacgacc atcacctgca
     4981 cattgggatt cggaaacttt tccagcgaga cgggcgcttc ctgtgtggtg atcccgagaa
     5041 cggcgttgga gcccgcgagc tgatacacga cgaacggacg gcccgcctgc ccgatccggt
     5101 acagccgcgc ccgcccctgc cggtcgaaat acgacaggag ctgcatcccg ttcaccgtcc
     5161 gtctatcgag caccggatcg agaacggtcg gcccgatcac cagcgtgtcg ttccgaattg
     5221 ccgcgggaaa ctcgtcggtc gcgttcccgt tgccgttgat atcgtacgcg aagcgcgaca
     5281 tcagaaaatc gcgttcgagg atcttcccgt gcgcgttccc ggcgatcacg atccgctccg
     5341 tggtgatggg aacgctcgcg ccgctgaaga tcgtcgcggg cccgcgatag gcgatgcgaa
     5401 gatcgacccc ataccctgcg ccggccttct tcacggtaca ggcgtcgatc gtgaacgaaa
     5461 acgacgagcc cgccacggcc gggagcgtcg cggacaaaaa cagcactgcg actaacaaca
     5521 ttcttccagt catgcatcct ccctcacgcc tgcgaatcaa acagcttcga tgcgcgcggt
     5581 acccgcgtat tcgcctgtca cgcaccgctc gttcgggaaa gaaattcatc agcagagagg
     5641 tgatgatagc gttaccgcca cgaacgtacg gcccggttca ggagcgttcc tccagcaatc
     5701 gcccgaagat gcgtttcttt ttcgcgcacc atgccgtaaa cgcgaccggg tcgtctcgtt
     5761 ccggatagcg cgcatagtgg tcgaggacgc gccgcagcgt catgcccatg cgcgcgatgg
     5821 gcggaaggag cgtcgggtgg gccgagaagc cgcgaattcg cgcgggaacg gtgcgcgccg
     5881 atatccgcgg ctcgttcacc tgaaggccgt tgagcgtgtc ctccgcctgc atgatgccgc
     5941 tctcgagcat cgaggcgaca tcgtccgccg agagcgaatc gaccgtgagc cgcgtaacgt
     6001 catagccggc cagtcggcgc ccccgcccgc gctgataggc ggccgcatac gaccagaggg
     6061 cggccgcgga gacatcgttc ccgcgaatgg cgttcaccgc gacgcccgac gcgagcgtgg
     6121 cggtgatgag cgccgacgcc acgccgctgc cgtgcatcgg gaataccgtg caggccgcct
     6181 ccccgagcgc catgaagccg ccggcgacca ttcgatcgag cgaatgccgg atgcggatcg
     6241 gagccgccgc cgcgcggacc tgtgtgccga agcaccccgc gtcgcggacg aatgcatcga
     6301 ccacctgccg cgccgtgatg atgcgctcgt gatcgcgctt gaggccgacg agcagatagg
     6361 ccgtcccatc gcgtttcgag aggaaggaat acaccgtcga ataggagccc gtggtcccca
     6421 tgcgcgcaat gatctcgtcg ggtgcgaaca ggccgttgcc gatcgccgca tcggccttcg
     6481 cgggaacaag ccgatgcaga cagttcgcgg cgctcaccac atggcgggac gattccggaa
     6541 actcgaagcc aaaggatgcc ggcagcgagc gcaccaacgc cgcggcgaac ccggtcgcat
     6601 cgatggtgat ccgcgcattc acgtgacgat cgcggccgtc cgaccctttg cagatgaatc
     6661 ccgtgacgcg ctccccggag gtgatggcgg aaatcacgcg cgtccccgga acaaagcgcg
     6721 caccggcccg tttggcctgc acgagaagtc ccttgacgaa gacatcaagg ttgatcgcca
     6781 ccggaagcgc gtgctcgcgc tcgtcgaacg agaagagctc gcggccccgc gcggagagaa
     6841 cgcgcaaccg cgcgcattca tagggcaccg tgtcgccgga cggaagcggc agccccacct
     6901 ccgaataaac cgtcggttcc gtttcaagga cgagggaccg tcccagcccc acctcgggaa
     6961 gcgcatcgaa caatccgacc gagaggcccg cctccgccag gcgcaatgcc gcgagcgtcc
     7021 ctccaggccc ggcgccggcg atcgcgacat cgaaatcgaa acgcttcgtt ttcatcgtcc
     7081 ccctctttta ccgcacacgc gtgcttctac aacaccatcg tgagcgccaa ctgtcaaagc
     7141 attaattgca gaccaaatat gtaattttct tgctttctcc aatgacgcat gtatacaatg
     7201 atatagcgcg acacggtcat ggagttcatt gaatgcaacc gatcagaatt caggcaacgc
     7261 cccccaggga tatcgcagcc tatcgcgaca tcgtgaagcg cggtctcggg ccctattttt
     7321 tcttcggctg gttctcaatc acgggaccgg cgataccgac cgccatgatg cttgcacatg
     7381 cgatcatcgg cgatgcaccc gaggagatat tcgttcccgg agtcattgct ttcgtcgtcg
     7441 cgggcgccat tctgggaacg atactcctgc gcagcgggag caacatcaag cgcgaacggg
     7501 aactggccct ttcccggggg gtgatgatac cggccacggt cctcgcgcac ggcagggcat
     7561 ttgcgttcta caagagcgtc cgcgactaca cccttaccgt tgaatatgca ggccccgatg
     7621 gcgcgccgca ccgctcgatc atccgctcat cgaacagaga gctccaccgg catgatccgg
     7681 gcgacacgat catgggactg cgagacgaaa cgagcggcgc tgttttcttt cccgccgaag
     7741 ccgggtgcga acttcacgcc tgatggcgtc cgcaaccatt tgaaaaatac gcgccgctac
     7801 tgacgaagct tcggcgaatt aaaccagagc cccgagacga ggtcccggtc gcctatcttc
     7861 tggaacacga ccttgatctc gaccccgtcc tcgcgttcga acttcccctt gtatttcacc
     7921 accacgaact gatctttctg cgaaacatcg gacacctcgc gagagagata cttcccgatc
     7981 ttcgacatga tgagctctcg tgtctggaca aatacgttct ccggcattgc gttcttcatc
     8041 tgcgcatcga aatccgttac gtagcgggca taggttccgt cgttatacgc cgcgaggatc
     8101 ctgtcgctcg cgggattcgc gtatccaagc accgcctcgc gctccgcccc ggtgattgac
     8161 cgcttaccgc atccggcagc gagcagcaag atgatgagaa ccgcaacgac ttgatgtttc
     8221 atggcgtccc ccctgtatca catcctggat agtgtgtgtt cagcattggc ggcgaatgta
     8281 tgatatgcgt gcatcttgaa ttcgcgagcc tcagtccgcg cacatgcgca atacctcgat
     8341 cacgccgcaa ataccacacg tacgatacgc cgcccaacgc cattgtaaag ataaccgcaa
     8401 cggaagcaaa aagaaacaaa aatattatct gaacgaaccg gaaaccggcg cacgaaggaa
     8461 tgatgaggcc gttgaaaacg ctacgatgcg gagtccgcct caagacggcg ccgaatgatc
     8521 atttttatcg cgatagcgat gataatcacc ccgacgagaa cgcccaccgc gacatgaccc
     8581 tgcgtcatcc attccatgag ccgctcgcga ttgtgcgcgc cgaaataccc gaccgcaagc
     8641 cacaccggaa cgctgatgag cgcggcgaat ccgtcgatcg cgaaaaaggc gccgggaggc
     8701 acgacccgca ggataccggt cgtgaggaag atgggcgagc ggaacccggg cataaagcgt
     8761 ccggcgaaga tcacccagcg cccgtacttc ccgaaccagc cctgtatcgc atgaaagcgc
     8821 ggaccgttga caagcgcccg cgtaaaccgg tactcgaggg ccttcatgcc caggtagcga
     8881 ccggcgaaat aaatgatccc gtcgccgata agaacgctcg ccatcacccc gacgcccatc
     8941 acgcgaatgt cggtgaagcc cagccccgac agcacgccgc ccgcgacgat ggagatgtcc
     9001 tcgggaaccg gcaggccgag gccgcacaaa agcagcatcc cgaagaccgc ggcatacccg
     9061 taggtctgaa agaatacgat gatggtatcc agttcaatca tgattggtgt ccgatatcac
     9121 tacacgcgtg taaataatca ataaagattt cgcaaaatac gcgcgtgaac gaatcatcac
     9181 acaataaaaa gcttgaaata attggctcgc ccggtgagta ttcattcatt gattaatcat
     9241 gcgcctacac catagccgag gtaccccgac gatgagacca caccgccatg tccgttgcat
     9301 gatcgccgcg ctcgccgcat tcacgatcgt ctcctgccgt ctcgaagtcc cggtccgcga
     9361 gatggcgcag gcgaaatcga ccatcacgcg cgcgcacgag gtgaaggcgc ataaatacgc
     9421 cccggacgag ctcaaaaagg ccaccgacca tctcatgagc agccatcgct ttgcggcagc
     9481 aggcgataaa ggcgcggcga cggataccgc tctcaaatcg ctctcattcg ccaataccgc
     9541 ggtcgcaaaa tccctgccgc tcctctcgaa ggactcgctc gacgctgcga cggccaaggt
     9601 gaacgaggcc gcctccctcg ccgatcccgc cgcaaaaccg tcggagctcg tcgacgccga
     9661 gcagaaactc gcgcgcgccg ggcaactcca ttcggaatcg aagttctggg aatcgtacct
     9721 tatatcgaag gacatcgctc ccgcccttga cggcttcatc ggcaaacagt cggaagacct
     9781 gaaacaaagc gccgcgcaac ggctcgaggc ggcgcagaac gccctgcgca cgtgccgcgc
     9841 tgcgggaccg gacgattcgc aaaaagccga tatcgacaag gccgccgcgc tcatcgacaa
     9901 ggcaggcaac gcgctttcat ccggcgccta caaggactcg attgcggcct ccgacgaggc
     9961 catttcgatc ctgaacgccg cgaccatcgc catggagaag aagcgcgaag cgacgattat
    10021 cgacaccaaa aaggaaaagg aacttacgga aaaaaccgac actgaccgcc ggacctacaa
    10081 ggtcgtatac aaccccaaga agcgcgattg cctctggcgg atcgcactta aaatgtacaa
    10141 gaacccgagg ctctggccgc ttatctacgt cgccaacaag gacaaaatta aggatccgga
    10201 tctcatctat ccgggacagg tgtttatcat cccgcccgtt ccaagcaaaa agccttcatc
    10261 cgatcaaaag acggatgggg agagcgatga tgatgacgaa gacgacgata ccgagagcgt
    10321 agacgaaacc aacgcgactg ataagtccac cgattcggcc gacaagaaac catctgacaa
    10381 gacagagact ccatcgccga agaaaaatgc gcccgccgac gcaacggacg ctccggagga
    10441 ctacagcgac gaagacgccc cttgatcacg cgacatcacg ccattatgct tggtcgatga
    10501 acgcaccgag cctgcatacg caggctccat cataccacac gagggaaaca tcatgcgcat
    10561 gatacacaca atcgccacgc tcaccatcgc cctggtcgtc gccgcgaccg cgcaggcgaa
    10621 caccctgggc gcaaaacagg acacgcggcg cattgccgat gaattcatga aaaagattca
    10681 gacgggcgat ttcgacgccg cgttcggcgg catcaagatc tttttcccga tccccgagga
    10741 cgagttcaac aacctggtca cgcaaacgaa gaaccaattc caaatggtaa aaaaccgttt
    10801 cggaacaacg atcggctacg agcttttcaa ggaagagtcg atcaaggacc tgatggtcaa
    10861 gtatacgtat atcatgaaat tcgagaaaca catcatacgc tggatgttca tcttctacaa
    10921 accgacccag gggtggctgc tcaactggtt ccactgggac gacaaaatcg acgccctttt
    10981 caacgaaccg ctcgaaaata acaaaaaata gggcgttttt atttgcccct gcatgacgcc
    11041 gttgctattt taccggcatg ggcgcccaat cctacaatac aacgaaatcg tcgaacaagc
    11101 cgcgctgcaa gctcccggag ttgtgcacct cgtcgtatct catcggaaac acggtgtttg
    11161 ctctgcgcga cgcagggcga gagcgcgatg tcgcagagtt tctcagccgc ctcaagccct
    11221 acgaggacga gataaacttt tcgattgttt tggaagttgc agtcgactac gtggaatttt
    11281 acgactaatc cgtccggccg tctcgaagcg cttcaacggt ttcaggtaag cgcaagtatc
    11341 acgatcactc cgatgacgat aaccacgatt cccgcgacaa tgccgatgat gaggctcatg
    11401 cgcgattttt ttatttcctg ctccacaaag ggatcgcccg ccttgatgcg gatgtcggcc
    11461 tccacctcgc acttgccgat gaggcccttg gcgatattca cgtacaggtt atagccgtct
    11521 ttcgtcttct tgatgctgtg cacatgggcg ctcatcggtt tgatcgtgtc gccctcgatg
    11581 aggttgagcg atcgcgccaa tcgcatcgag accgtattgc tcttgtccac gcgcacctgc
    11641 acctggtccc ccgggaccag cgacgtgagc ggcctccccc gcgccggcga aaggaccggc
    11701 agcacgggta tgattgtcgc ctccggcatt ctatccggat ccttgccgtc cacaccgccc
    11761 gcctggttga ggtcgaaaag atacccagcc accaacgagc gcatggtttc ggcgcgcacg
    11821 gcgacctgga ccttgccctt caggacatcg ccaagcaccg tggccatcat ctcgcgtacg
    11881 cgctcggtat cggcccccga gaggatatcg tgaag
//
LOCUS       DAORHF010000104        11882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4522_length_11882_cov_6.375328, whole genome shotgun sequence.
ACCESSION   DAORHF010000104 DAORHF010000000
VERSION     DAORHF010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4522_length_11882_cov_6.375328"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PKJ16_09975"
     CDS             <1..198
                     /locus_tag="PKJ16_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47358.1"
                     /translation="QPAVGVDHESDPTLGYLDPPHFPSEKRARVEISFMVNADRWLCA
                     SVYDLKTQKYLLEQKPVIRLK"
     gene            complement(274..1134)
                     /locus_tag="PKJ16_09980"
     CDS             complement(274..1134)
                     /locus_tag="PKJ16_09980"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV47359.1"
                     /translation="MAASIIETMQRRRSCRTYRKGTLDERSRALLEDEIARATRGPFG
                     SDMRLSLVAANGDDAAALKGLGTYGMIKNPAGFIIGALRESPSCFEDFGYAMESIVLG
                     ATAAGLGTCWLGGTFKRSRFAERMALAEDEIIPAVVSFGAPADRRALESIVRSFAGSD
                     HRQPWGALFKDGDFSAPLPEAAGPYRVPLEMVRIAPSASNRQPWRVVADRDGRAFHFY
                     LQRLKGYKERNHAFFGIVDMQRIDMGIAMCHFELAATEAGLRGRWAIRDPGIAPLPVD
                     CFYTASWTAE"
     gene            complement(1289..2776)
                     /locus_tag="PKJ16_09985"
     CDS             complement(1289..2776)
                     /locus_tag="PKJ16_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HNV47360.1"
                     /translation="MTKRTIAVCVICGVFGVTGVVLLANGCAPSRPELVKVASIPDGE
                     YDPAEWGKAYPLEYESWAKTKEPRPTGKSKYKPGLDTDKIVYDKLSEFPFMALLFHGW
                     GFGVEYNEPQGHWYMRIDQDIIDQSRTKAGGVCLNCKSPYMDALVKEHGPGFYSMPWQ
                     EAVAKIPAKHKDLGAACIDCHDNRTMDLKINRAAFSKGLAKLGKKEFTRQEMRTLVCA
                     QCHVTYNVPKDQNMKSLGVDHPYDGSTWGTITIENIIAVIKRTPAYKEWTQAVTGLKV
                     GFLRHPEFEFFTRASVHWNAGLSCADCHMPYQKAGANKISNHNLMSPLKDDLRACVKC
                     HPQGAERLKRQVIETQDRTVSLLLRAGYGTATAAKLIELANAAPRQGKTVDQGLYAQA
                     VDFYLEALYRVIFIGAENSLGFHNPMEAGRILGDAIAFSAKSEALLRQLLAAAGVTVP
                     ANIMLDLTKYINNRGTHRKNFRKEFEFADPFGIQERILPRAARGI"
     gene            complement(2801..3358)
                     /locus_tag="PKJ16_09990"
     CDS             complement(2801..3358)
                     /locus_tag="PKJ16_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006918888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HNV47361.1"
                     /translation="MNSVRTILSNLFQAIARVRSAAADGLASLAERDRSASRRRALRI
                     ITNRYVLASLALVLVGMGAAAPFLVRITSTPGFCGSCHVMDDQYEHWFYDGKHRMISC
                     VDCHLPNNNIVNHLVWKGIDGMKDVFFFYTRLTPEIIRASSHARRTIRANCVRCHDEM
                     VSRVSIDTMQCWDCHRARNHQVVAQ"
     gene            complement(3429..4034)
                     /locus_tag="PKJ16_09995"
     CDS             complement(3429..4034)
                     /locus_tag="PKJ16_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HNV47362.1"
                     /translation="MATREADNGDIDRTIAAALDKLTQVQRILLWDIAKHEKLSPIQI
                     QFLLHLQRYPDALRTVSVLAREFDLTKATVSDALSALEDKGIIARERDEADRRSHIVR
                     LTAKGARLADRVGPWQDALVRHIGRLPRADRERLLGSLMTLIKSLHDDGVISVARMCI
                     ACENFVRGRDGEPHRCVLAGHAIAEADLSIGCAHYRSASTG"
     gene            complement(4105..4350)
                     /locus_tag="PKJ16_10000"
     CDS             complement(4105..4350)
                     /locus_tag="PKJ16_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47363.1"
                     /translation="MNAHCPGAASLRTPELAVKRCPACGADVELFSNEPCAECPVCAF
                     TVYRDTASCIAWCRYAEECVGTETYRRLMQRAADKTD"
     gene            complement(4347..5222)
                     /locus_tag="PKJ16_10005"
     CDS             complement(4347..5222)
                     /locus_tag="PKJ16_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47364.1"
                     /translation="MKRQIINIDDTLCDGCGNCVPGCPEGALQIIDGKARLVSDLFCD
                     GLGACIGECPQGAITVIEREAEPYSERTVMENIVRQGPGTIRAHLAHLRDHNETGYLN
                     EAIAVLREKNIPVPEEFVGSDAKRFQADIPCGCPSSHARVVVPHKTAGVDSGYEAPSE
                     LSQWPIQLHLVNPRNPYFAGADLVVAADCVAFSHGDFHRRFLKGKKLVIFCPKLDDGV
                     DAYIDKLAELFATQGVRSVTALHMEVPCCFGTVKIVQEALRRSGADIPFEEFTIGIDG
                     AIQGRKEQRLAGARA"
     gene            5376..7931
                     /locus_tag="PKJ16_10010"
     CDS             5376..7931
                     /locus_tag="PKJ16_10010"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47365.1"
                     /translation="MNPALLDNVLLTHNAFITATAAMFTVLVGAFILSIKRKSSASAH
                     LGLALIAISFTNIAFTVAFAWYHPLAAYHRWLTMLPVLLGIGHLAIFFFYFPTDRNRT
                     AALIFIAAVWIVAIVDEVVWVRQTLHFTKYFQPDGQIWDVEESRLAAINAALIMSYIV
                     AAIVTGAWRVVASRKRERAPAAVMLACFTIVTILPGIANAMARQGALERDVFILAYAL
                     ISVLGWFSSVIVYLNLTRDQTTFMAKIMGVTLATVLVILMGISFFTSRDADRLYDELR
                     VREAERIAADPSYRPPGVASVIAYSNATGETRPLQGAGPVAFDRAARAEEFASVIALE
                     RIAALPAGFDPRALETILENAPSHFTGYGAVIRTALHSDGPSPRPGGTAAVKRLAGLA
                     RARSNLFYKVQVLPDERFRQELARLLSEDHRGLAPFTRAIDAHLRARDSEGTALKREV
                     LRYLAPIPRAGSRIIRTDAATGEHRLSFIIHPAGSERLYEVGFSYRGYREYVHASAVR
                     FFIILGLVLVAVLAGFRLFFLGALTRPLERLLNGVAQVEQGNLDVRLSVAAMDEIGFL
                     TKNFNRMVRTIRMTRKKLYRYTTTLEEMVHDRTAELQSARDALWGEMQLAMKIQTVLL
                     PTAPSIPGYEIDVTMSPASEVGGDYYDVINAAGRHWVIIGDVSGHGVPAGLIMMMIQM
                     AIHTAVSRDPAISPAELLTDINAIVTGKMKLISEDKYMTITAMSCGEGGRFTFAGLHQ
                     DILIFRAATGTVEAVETQGMWLGIAKNLRGHMRDDTLTLGVGDVMLLYTDGITEARRG
                     EHGAELFGSLRLRETFARAGHGSSAEIRRAILDAVEGYHRFDDVTLMVIRRTG"
     gene            complement(7952..8725)
                     /locus_tag="PKJ16_10015"
     CDS             complement(7952..8725)
                     /locus_tag="PKJ16_10015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HNV47366.1"
                     /translation="MVDAKQRNMGTSDTADNLLINRIQKAPDIPNLVVEQILRLIATG
                     ALRPGDKLPSELEMTRRFGISRISLREAMKLLEAKGFIESQGRKGKFVRSLVDDAVQS
                     PISGLLAADHQKIWELLEVRRFLDAQAAALAAERATEYQVKRLVDFVRNAERMGIENI
                     IDRRESGKLYAEFYSELAEATNNTIFEHLVKSISNILQGALPYSREKLKGLKDTSQRI
                     YTHHKKLVEAIQRRDPEAARRAVLEHIDYIERSLKKVLR"
     gene            complement(8810..9400)
                     /locus_tag="PKJ16_10020"
     CDS             complement(8810..9400)
                     /locus_tag="PKJ16_10020"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HNV47367.1"
                     /translation="MKIAHLFEGRILTISNLLSVLRVFLVPPMGYCFYLFKQTGDPRY
                     NYLALGVGIVIMLTDYFDGYLARLLKQETMLGRFLDPLADKFTFYITGTLLYWYYGYP
                     LWVILAVLARDIFALAGGYLIFAKKNIQGTPNRAGKWAVFFMGMSAVAYILAPTVNLY
                     GITLQHASLALLVVCLVVSLVQYLVTYGALFSTGDR"
     gene            9556..10413
                     /locus_tag="PKJ16_10025"
     CDS             9556..10413
                     /locus_tag="PKJ16_10025"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV47368.1"
                     /translation="MIVRSGARASLIILLAVLVAAGALACKKSRSDALTADENLGLII
                     RDNTALRLDPLFYSSRISVLKKGETVAILDRSKKKGYIGTSGQYWYKVALKSGITGWV
                     FGGTIRVLSDKNASAVEDQISDLMQEESKQLRRTLAGKWWSIDSFGEFTNHILDIEES
                     GKYKSSERGGKSIEGKFQIRVRDQEIVFDKGTSFERNLFYARRGVSYFLENNAKDKSG
                     PIKFKKLVGPESLEEANKYQQGAGADKPKEQAPDKPTEKAPEGGAAKQPPSTTPPTAP
                     TDGTNPPNK"
     gene            10388..11146
                     /locus_tag="PKJ16_10030"
     CDS             10388..11146
                     /locus_tag="PKJ16_10030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HNV47369.1"
                     /translation="MEQTLRINKYLARCGLGSRRKVEQLVLDGKVSVNGTIIRDLASC
                     VDPERDTVALGGEIVKPSGRRFYLVLNKPRGYVTTVADDRGRPIVMDLLPERYRTAGV
                     VPVGRLDQDTEGLLFLTNDGDLAHRLLHPRFHVEKEYLVELDRDLDDVDRARIEKGIM
                     LYGHRTRPARIEFPTERRATVLMAISEGKKRQIRLTFKAFGYTVKLLRRITFATLSLA
                     RLPRGMHRALRPGEVKKLMAHAGCAPEPSDDARK"
     gene            11256..11843
                     /locus_tag="PKJ16_10035"
     CDS             11256..11843
                     /locus_tag="PKJ16_10035"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HNV47370.1"
                     /translation="MDLYTGRNARSAPSFPAHDPEYFTRMAAANHRDEERNKKKASRM
                     FSFILALCIISFTTGLVVGIKFTAGANKQLVDDRTAHKVGEIGTKVKGMIATAPKTTT
                     PDDAAAVKKKQLFPKSEFPFVILVGRGLEKRRADEIASFLSTKGHTVIVSKSGGAYTL
                     YTGPYRDRSDASAAQRNLGEYGKPDWFSRAEVLSR"
BASE COUNT         2181 a         3957 c         3657 g         2087 t
ORIGIN      
        1 cagccagcgg tcggcgttga ccatgagagc gaccccaccc tgggctacct ggaccccccg
       61 cactttccct cggagaagcg cgcgcgcgtg gagatctcgt tcatggtcaa cgccgaccgc
      121 tggctgtgcg cctcggtcta cgacctcaag acgcaaaaat acctcctgga gcagaagccg
      181 gtcattcggt tgaagtagga tggcggcgtg ccgccgctga tcgccgccgg tgccgttatc
      241 cgaaacagat accgtcattc ggcgaacgcc gcgtcactcg gcggtccagc tcgcggtgta
      301 gaaacaatcg acgggtagcg gcgcgattcc cggatcgcgg atcgcccacc gcccgcgcag
      361 gcccgcctcc gtcgcggcga gttcgaaatg gcacatcgcg atccccatgt cgatgcgctg
      421 catgtcgacg atcccgaaaa acgcatggtt gcgttccttg tatcctttta agcgctgtag
      481 atagaaatga aacgcgcgtc cgtcccggtc ggcgacgacc cgccatggct ggcggttcga
      541 ggcggacggc gcgatgcgca ccatctccag cggaacgcga tagggccctg ccgcctccgg
      601 gagcggcgcc gaaaaatcgc cgtccttgaa gagcgcgccc cagggctgcc gatggtccga
      661 acccgcgaag gatcgcacga tggattccag tgcgcgacgg tccgccggcg caccgaacga
      721 cacgacggcg ggaatgatct cgtcctccgc gagcgccatc cgctcggcga agcggctgcg
      781 tttaaaggtg ccgccgagcc agcaggtccc caggccggcg gcggtcgcgc ccaggacgat
      841 cgattccatc gcatacccga aatcctcgaa acacgacggc gactcccgca gcgcgccgat
      901 gatgaacccc gccggattct tgatcatccc ataggtgccg agccccttga gcgcggcggc
      961 gtcgtcgccg tttgcggcga cgagcgacag acgcatgtcg cttccgaacg ggccgcgggt
     1021 cgcgcgggcg atctcgtcct ccagcagcgc gcgcgatcgc tcatcgagcg tccccttccg
     1081 ataggtgcgg catgaacgcc gtctttgcat tgtttcaata atagacgcgg ccatgaatac
     1141 ctcctctgct gcagtacacc aaatcccgat cggagatcga atggaacgtc acgccagtgg
     1201 ccatgcgccg aatgaggcac cgcttctcgc gcgatgacgc cgagtcatac tccacgcctc
     1261 atcattacca gtaccggcct tggcgcggct atatgcctcg cgccgcgcgc ggaaggatcc
     1321 gctcctgaat gccgaaggga tcggcaaact cgaattcctt gcggaagttt ttccggtgcg
     1381 tcccgcgatt gttgatgtac ttcgtgaggt ccagcatgat gttcgcggga accgtcacgc
     1441 cggccgcggc gaggagctgc cgcaggagcg cctccgattt cgccgagaag gcgatcgcgt
     1501 cgcccaggat gcgccccgcc tccatcgggt tatggaagcc cagcgagttc tccgcgccga
     1561 tgaagatcac gcggtacagc gcctcgaggt agaaatcgac cgcctgtgcg tagaggccct
     1621 ggtccacggt cttcccctgc cgcggcgcgg cgttcgcgag ctcgataagc ttggccgcgg
     1681 tggccgtacc gtaccccgcg cgcaaaagga gcgagaccgt gcgatcctgc gtctcaatca
     1741 cctgccgctt caggcgctcg gcgccctgcg ggtggcactt cacgcatgcg cgcaggtcgt
     1801 ccttgagcgg gctcatcagg ttgtggttcg atatcttgtt cgcgcccgcc ttctggtagg
     1861 gcatgtggca gtcggcgcac gagagccccg cgttccagtg cacgctggcg cgggtgaaga
     1921 actcgaactc cgggtgccgc aggaagccca ccttgagccc cgtgacggcc tgcgtccact
     1981 ccttgtaggc cggcgtgcgc ttgatcacgg cgatgatgtt ctcgatggtg atggtacccc
     2041 aagtgctgcc gtcgtaggga tggtccaccc cgagcgactt catgttctgg tccttcggaa
     2101 cgttgtacgt cacgtgacat tgcgcgcaca cgagcgtccg catctcctgc cgggtaaact
     2161 ccttcttccc cagtttcgcc agccccttcg agaaggccgc gcggtttatt ttcaggtcca
     2221 tggtccggtt gtcgtggcag tcgatgcagg ccgcacccag gtccttatgc ttcgcgggga
     2281 tcttcgcgac ggcctcctgc cagggcatcg agtaaaaccc cggcccgtgc tccttcacca
     2341 gcgcatccat gtagggcgac ttgcagttga ggcacacgcc ccccgccttg gtgcgcgact
     2401 gatcgatgat gtcctggtcg atgcgcatgt accagtgccc ctgcggctcg ttgtactcca
     2461 cgccgaaacc ccagccgtga aacagaagcg ccatgaaggg gaactcgctc agcttgtcgt
     2521 agacgatctt gtcggtgtcg agccccggtt tgtacttact tttgccggtc gggcgcggct
     2581 ccttcgtctt cgcccatgac tcatactcca gcggatacgc ctttccccat tcggcgggat
     2641 cgtactcgcc gtcggggatg gaggcgacct tcaccagctc agggcggctc ggcgcgcagc
     2701 cgttcgcgag cagaacgacc cccgtaacgc caaacacccc acagataacg cagacggcga
     2761 tcgttcgttt cgtcatgtca tgtgctcctt tcgttcatgg tcactgcgcc acgacctgat
     2821 gattgcgcgc ccggtggcag tcccagcact gcatcgtgtc gatgctcacg cgcgaaacca
     2881 tctcgtcatg gcagcgcacg cagttggcgc gtatggtgcg gcgcgcatgc gacgacgcgc
     2941 ggatgatctc cggcgtgagc cgcgtgtaga agaaaaagac gtccttcatc ccgtcgatgc
     3001 ccttccagac gagatggttc acgatattat tgttgggaag atggcaatcc acgcaggaga
     3061 tcatccggtg ctttccgtca tagaaccagt gctcgtactg gtcgtccatc acgtggcacg
     3121 atccgcaaaa accgggggtc gaggtgatcc gtacgagaaa aggagcggcg gcgcccatgc
     3181 ccaccagaac aagcgcgagc gacgccagca cgtaccggtt cgtgatgatc cgcagggcgc
     3241 gtctccgtga ggccgatcgg tcgcgctccg cgagggacgc caggccatcg gcagccgccg
     3301 accgtacccg cgcgatcgcc tggaacaggt tcgaaagaat ggtgcgcacg ctattcatca
     3361 gggcaatcga tatacccctg cgccggatat atcaaacctt ttattcgaaa aaaaccgcgc
     3421 gtttttcgct accccgtgct cgccgaccgg tagtgcgcgc agccgatgga aagatcggcc
     3481 tcggcgatgg cgtggccggc caggacgcag cgatgcggct ccccgtcccg cccccggacg
     3541 aaattctcac aggcgatgca catccgcgcg acggagatca ccccatcgtc gtggagcgac
     3601 ttgatgagcg tcatgaggga gccgagcagg cgctcgcggt ccgcgcgcgg cagccggccg
     3661 atatgccgga cgagcgcgtc ctgccagggg cccacgcgat ccgccaaccg cgcgcccttc
     3721 gcggtgagcc gcacgatgtg cgagcgccgg tcggcctcgt cgcgctcgcg ggcgatgatc
     3781 cccttgtcct cgagcgcgga gagcgcgtcg ctcacggtgg ccttggtcag atcgaactcg
     3841 cgcgccagca cgcttacggt gcgcaacgca tccgggtagc gctggaggtg cagcaggaat
     3901 tgtatctgga tgggcgagag cttctcgtgc ttcgcgatgt cccagagcag gatgcgctgg
     3961 acctgcgtga gcttgtccag cgccgccgcg atcgttcggt cgatgtcccc gttgtccgct
     4021 tcccgtgttg ccatgacgat tgtgtctcct ccgcgctcga cgccgtgtgc gcccataaat
     4081 catctcatgc cgcgggcggg cgtgtcaatc cgttttgtcc gcggcgcgct gcatcagccg
     4141 ccggtaggtc tccgtcccca cgcactcctc cgcgtaccgg caccaggcga tgcacgacgc
     4201 cgtgtcccga tacaccgtga aggcgcagac ggggcactcg gcgcagggct cgttggagaa
     4261 gagctccaca tcggccccgc atgccggaca gcgcttcacg gcgagctccg gcgtgcgcag
     4321 gctcgccgcg cccgggcagt gcgcgttcat gcgcgcgccc ccgcgagccg ctgttccttc
     4381 cgaccctgta tggcgccgtc gatgccgatg gtgaactcct cgaacggaat atcggccccc
     4441 gaacggcgca gcgcctcctg gacgatcttc acggtgccga agcagcaggg cacctccatg
     4501 tggagcgccg tcacggagcg gaccccctgc gtcgcgaaga gctcggccag cttgtcgatg
     4561 taggcgtcga ccccgtcatc gagtttgggg cagaagatga cgagcttctt gcccttcaag
     4621 aagcgccgat ggaaatcgcc gtgactgaac gcgacgcagt cggcggccac gacgagatcg
     4681 gcccccgcga agtagggatt ccgcggattg accaggtgca gctgtatggg ccactgcgaa
     4741 agctccgacg gcgcctcata ccccgaatcg acccccgccg ttttgtgcgg gaccacgacg
     4801 cgcgcatgcg aactcgggca gccgcagggg atgtcggcct ggaagcgttt cgcatcggaa
     4861 ccgacaaact cctccgggac gggaatgttc ttttcccgca acacggcgat cgcctcattg
     4921 aggtagccgg tttcattatg atcgcgcaga tgcgccaggt gcgcgcggat ggtgcccggc
     4981 ccctgacgca cgatgttctc catgaccgtg cgctcgctgt aaggctcggc ctcgcgctcg
     5041 atgacggtga tggcgccctg cgggcactcg ccgatacagg cgcccaatcc gtcgcagaag
     5101 agatcgctca ccaggcgcgc cttcccgtcg atgatctgca atgccccctc cgggcagccg
     5161 gggacgcagt tcccgcatcc gtcgcacagc gtgtcgtcga tattgataat ctgtcgtttc
     5221 atggcacctc cgcaattagg ccactggtat ataattagga atcctaacca atatagcgac
     5281 gccggataaa atcgtcaagt aaaagtgaaa aaaaccttgt taataattca tttgtatgat
     5341 agtccccagg aaggcatacg acgcccgtat cggcaatgaa cccggcacta ctcgacaacg
     5401 ttctgcttac ccacaacgcc ttcatcacgg cgacggccgc catgttcacg gtcctcgtcg
     5461 gcgccttcat cctttcaatc aaacggaaat cgagcgccag cgcgcacctg ggcctcgccc
     5521 tcatcgcgat ctcgttcacc aatatcgcct tcacggtcgc cttcgcatgg tatcacccgc
     5581 tcgcggccta ccatcgatgg ctcaccatgc tccccgtgct gttggggatc ggccatctcg
     5641 cgatcttctt cttctacttc cccaccgacc gaaaccggac ggccgccctg atattcatcg
     5701 ccgccgtctg gatcgtcgcc atcgtcgacg aggtcgtctg ggtgcgccag accctgcact
     5761 tcaccaaata cttccagccc gacggacaga tatgggacgt ggaggagtcg cgcctggccg
     5821 ccatcaacgc ggcgctcatc atgtcctaca tcgtcgcggc catcgtcacg ggcgcatggc
     5881 gcgtcgtcgc gagccggaag cgcgagcgcg cgccggcggc cgtcatgctc gcctgcttca
     5941 ccatcgtgac catcctgccc ggcatcgcaa acgcgatggc ccggcagggg gcgctcgagc
     6001 gggacgtgtt cattctcgcc tacgcgctca tcagcgtcct gggctggttc tcgagcgtga
     6061 tcgtctacct gaacctcacg cgcgaccaga cgaccttcat ggccaagatc atgggcgtga
     6121 ccctggcgac cgtgctcgtc atcctcatgg gcatcagctt ctttacctcg cgcgacgccg
     6181 accgcctgta cgacgagctt cgcgttcgcg aggcggaacg gatcgcggcc gatccgagct
     6241 accggccgcc gggcgtcgcc tccgtcatcg cgtactcgaa cgcgaccgga gagacgcgac
     6301 cgcttcaggg cgcgggaccg gtcgccttcg accgcgccgc gcgcgcggag gagttcgcgt
     6361 ccgtcatcgc gctggagcgc atcgctgcgc tgccggccgg attcgacccg cgggcgctcg
     6421 agacgatcct ggaaaacgcg ccgtcccact tcaccggcta cggcgcggtg atccgcacag
     6481 cgcttcactc cgacggtccg tcgccgcgcc ccggagggac cgcggccgtg aagcgtctgg
     6541 cgggcctggc gcgcgcgcga tcgaacctgt tttacaaggt gcaggtgctt cccgacgagc
     6601 gcttccgcca ggagcttgcg cgcctccttt ccgaggacca tcgcggcctc gcgcccttca
     6661 cgcgcgcaat cgacgcgcac ctgcgcgcac gcgactccga ggggaccgcg ctcaagcgcg
     6721 aggtgctgcg ctaccttgcg ccgattccgc gcgcgggttc gcggatcatc cgcacggacg
     6781 ccgcgacggg cgagcaccgg ctttccttca tcatccaccc cgcgggctcc gagcgcctct
     6841 acgaggtggg cttttcctac cggggttacc gcgagtacgt gcacgcctcc gccgtgcgct
     6901 tctttatcat cctgggactg gtcctggtcg cggtgcttgc gggctttcgc ctgttcttcc
     6961 tgggcgcgct cacgcgcccg ctggagcgcc tgctgaacgg ggtcgcgcag gtcgagcagg
     7021 gcaacctcga cgtgcggctt tcggttgcgg cgatggacga aatcgggttc ctcaccaaga
     7081 acttcaaccg gatggtgcgc accatccgta tgacgcgcaa gaagctgtac cgctacacca
     7141 ccacgctcga ggagatggtg cacgaccgca cggccgagct gcagagcgcg cgcgacgccc
     7201 tctgggggga gatgcagctg gccatgaaaa tccagacggt gctcctgccg accgcgccgt
     7261 ccatccccgg ctacgagatc gacgtcacca tgtcgcccgc cagcgaggtc gggggcgact
     7321 actacgacgt catcaacgcc gcgggacgcc actgggtgat catcggcgac gtgtccggcc
     7381 acggcgtccc cgccgggctc atcatgatga tgatccagat ggccatacac acggccgtct
     7441 cccgcgaccc ggcgatctcc ccggcggagc tcctcacaga catcaacgca atcgtcacgg
     7501 ggaagatgaa gctcatcagc gaggacaagt acatgaccat cacggccatg tcctgcggcg
     7561 agggcgggcg gttcaccttc gcgggattgc accaggacat cctgatattt cgcgcggcga
     7621 ccggaacggt cgaggccgtg gaaacgcagg ggatgtggct ggggatcgcc aaaaaccttc
     7681 gcgggcacat gcgcgacgac accctcaccc tcggcgtcgg cgacgtgatg ctcctgtaca
     7741 ccgacggcat taccgaggcg cgccgcggcg agcacggcgc cgaattgttc ggcagcctcc
     7801 gcctgcggga gaccttcgcg cgcgccggtc acggctcatc ggccgagatc aggcgcgcca
     7861 tcctcgacgc ggtcgagggc taccaccggt tcgacgatgt cacgctgatg gtgatccgcc
     7921 ggacaggctg atcggtacgg tcgcgccgcg gttacctcag aaccttcttg agcgaacgct
     7981 cgatgtagtc gatgtgctcc aggaccgcgc gccgggcggc ctcgggatcg cgccgctgga
     8041 tggcctcgac gagcttcttg tggtgcgtgt aaatccgttg cgaggtgtcc ttgagcccct
     8101 tgagcttctc gcggctatag gggagcgcgc cctgcaggat gtttgagatc gacttcacga
     8161 ggtgctcgaa gatggtgttg ttggtcgcct ccgcgagttc cgaatagaac tccgcgtaga
     8221 gcttacccga ttcgcgccgg tcgatgatgt tctcgatccc catgcgctcg gcgttgcgca
     8281 cgaaatcgac gaggcgcttc acctggtact ccgtggcgcg ctccgcggcc agcgccgccg
     8341 cctgcgcatc cagaaagcgg cgcacctcga gcagctccca gatcttctgg tggtccgccg
     8401 cgaggagccc gctgatgggc gactgcaccg cgtcatcgac cagcgaccgg acgaacttcc
     8461 ccttgcgccc ctgcgattcg atgaacccct tcgcctcgag cagtttcatc gcctcgcgca
     8521 gcgagatgcg gctgatgccg aaccggcggg tcatctccag ctcggagggg agcttgtccc
     8581 cgggccgcag cgccccggtc gcgatgagcc gcaggatctg ctcaaccacc agattgggga
     8641 tgtcgggggc cttttgtatc ctgttgatga ggaggttgtc cgccgtgtcg ctggttccca
     8701 tgttccgctg ctttgcgtcc accatgttcc catcacccta gtgccaccgg ggtgggatgt
     8761 caatcaattt gtgatattgt cttacaaaga ggcatgcgcc gcaggatcgt taccgatcgc
     8821 cggtcgagaa gagcgcgccg taggtgacga ggtactgcac cagcgacacc accaggcaca
     8881 ccaccagcag cgcgagggag gcgtgttgca gcgtaatccc gtacaggttc acggtgggcg
     8941 cgaggatata ggccaccgcc gacatcccca tgaaaaacac cgcccacttg cccgcgcgat
     9001 tgggcgtgcc ctggatgttc ttttttgcga agatgagata cccgccggca agcgcgaaga
     9061 tgtcgcgcgc gagcaccgcg aggatcaccc agagcggata gccgtaatac cagtagagga
     9121 gcgtccccgt gatgtaaaag gtgaacttgt cggccagcgg atcgaggaac cggcccagca
     9181 tggtttcctg cttgagcagc cgcgcgagat acccgtcgaa ataatcggtg agcatgatga
     9241 cgatccccac gccgagcgcg aggtagttgt atcgcggatc gcccgtctgt ttgaacaggt
     9301 agaagcaata gcccatcggc gggacgagga acacccgcaa caccgacagc aggttcgata
     9361 tggtcaggat ccgtccctcg aacaggtggg caatcttcat gttcgccgct cctcgggata
     9421 ttcgcgccgc acacccgatg gcaaacgtgc cggacaatat cgttttttgc aattaaaata
     9481 cctgcggaaa aacgttgttg acggtttcgc gatcatcccc ggtagaatac ttttcttatt
     9541 aaatgggagg gcgtcatgat cgtgcgttcc ggtgcgcgag catctctgat aatactgttg
     9601 gcggtcctgg tggcggccgg ggccctcgcg tgcaaaaaat cgcgctcgga cgccctcacg
     9661 gcagacgaaa atctcggtct catcatcagg gacaacaccg ccctgcgcct cgacccattg
     9721 ttctattcct cgcgcatctc ggtgctaaaa aaaggggaaa ccgtcgccat cctggaccgc
     9781 tccaagaaga agggatacat cggcaccagc ggccagtact ggtacaaggt cgcgctcaaa
     9841 agcggcatca ccggctgggt cttcggcggg accattcgcg tgctctcgga caaaaatgcg
     9901 tctgcggtgg aggaccagat ctcggacctc atgcaggagg aatcgaagca gctccgcagg
     9961 accctggccg gcaagtggtg gagcatcgac agcttcgggg aattcaccaa ccacatcctc
    10021 gacatcgagg agagcgggaa gtacaagtcg agcgagcgcg gcgggaaatc catcgagggt
    10081 aaattccaga tccgcgtgcg cgaccaggag atcgtgttcg acaaggggac ctcgttcgag
    10141 cggaacctgt tctatgcgcg ccgcggcgtg tcgtatttcc tcgaaaacaa cgccaaggac
    10201 aagagcggcc ccatcaagtt taaaaaactg gtgggaccgg aatcgctcga ggaggcgaac
    10261 aagtaccaac agggcgccgg cgccgacaaa ccgaaggagc aggcccccga caagccaacc
    10321 gaaaaggcgc ccgagggcgg cgcggcaaag cagccgccgt ccacaacacc gccgacggcg
    10381 ccgaccgatg gaacaaaccc tccgaataaa taaatacctc gcccgctgcg gcctcggctc
    10441 ccgccgcaag gtggagcagc tggtcctcga cggaaaggtc tccgtaaacg gaacgatcat
    10501 ccgcgacctc gccagctgtg tcgatcccga gcgcgacacg gtcgcgctcg gcggcgaaat
    10561 cgtgaagccg tccggccgcc gcttttacct ggtgctcaac aagccgcgcg gctacgtcac
    10621 caccgtcgcg gacgatcgcg gccgccccat cgtcatggac cttctgccgg aacgctatcg
    10681 cacggcgggc gtggtccccg tgggccggct ggaccaggac accgagggcc tcctcttcct
    10741 gaccaacgac ggcgatctcg cgcaccgcct gctgcacccc cgcttccacg tggagaagga
    10801 gtacctggtg gaactcgacc gcgatctcga cgatgtggac cgcgcgcgca tcgagaaggg
    10861 gatcatgctc tacggacacc ggacgcgacc ggcgcgcatc gaattcccca ccgagcggcg
    10921 cgccaccgtg ctcatggcca tcagcgaggg gaaaaagcgg cagatccgtc tcaccttcaa
    10981 ggccttcggc tataccgtga agctgctccg acgcatcacc ttcgccacgc tctccctcgc
    11041 gcgcctgccc cgcggcatgc accgcgcgct gcggccgggc gaggtgaaaa aactcatggc
    11101 gcatgccggg tgcgcgccgg aaccgagcga cgacgcgcgc aaataatcct aatagtattc
    11161 cccgcgatgg ccgacatagc aactggcggg tccacagcgc cgcacggaca acgccatcat
    11221 atctcgggac caaggatcga cccgccatgc aaaatatgga tttatacacc ggcagaaacg
    11281 ctcgcagcgc gccgagcttt cccgcgcacg accctgaata tttcacgcgg atggccgccg
    11341 cgaaccaccg cgacgaggag cggaacaaga aaaaggcgag ccggatgttt tcgttcattc
    11401 tggccctgtg catcatctcg tttaccaccg gactcgtggt gggcatcaag ttcaccgccg
    11461 gcgcgaacaa gcagctggtg gacgaccgga cggcgcacaa ggtcggcgag atcggcacca
    11521 aggtgaaggg gatgatcgcg accgcgccga aaaccaccac gcccgacgat gcggccgccg
    11581 taaaaaagaa gcagctgttc ccgaagtcag agttcccctt cgtgatattg gtgggacggg
    11641 gccttgagaa gcggcgcgcc gacgagatcg cctccttcct gagcaccaag gggcacactg
    11701 tcatcgtttc gaagtccggc ggcgcctata cgctctacac cggcccctat cgcgaccgct
    11761 ccgacgcctc ggctgcgcaa cggaatctcg gcgagtacgg caagcccgac tggttctcgc
    11821 gcgcggaggt cctcagcaga tagccatgag cgcgcaggtg cgggaggccg gcccccaaga
    11881 cg
//
LOCUS       DAORHF010000105        11642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4696_length_11642_cov_4.633641, whole genome shotgun sequence.
ACCESSION   DAORHF010000105 DAORHF010000000
VERSION     DAORHF010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11642
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4696_length_11642_cov_4.633641"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..750
                     /locus_tag="PKJ16_10040"
     CDS             55..750
                     /locus_tag="PKJ16_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47371.1"
                     /translation="MNILQPDRLSDEVTCPYIPGNRCRYRYFFATDVTADELEHLLAT
                     GWRKFGPYYFMPECGECRECVPVRVLAREFAPSANQRKTLAKARAIETRFEPLAFSEE
                     IFDIYCDHSINRFGSNPSLDDFLHNFYYPSCPALQSEYRLGDSLIAAGFIDQSSRGLS
                     SVYFIYRTAHGRYSLGTVSAIREIEHARELGLDYYYLGYHVARNHFMAYKNRFRPYQR
                     FDWEKKQWAQPEE"
     gene            838..2043
                     /locus_tag="PKJ16_10045"
     CDS             838..2043
                     /locus_tag="PKJ16_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47372.1"
                     /translation="MKYLHRDAEKTIARYCSLFPAVALTGPRQSGKSTTLKKLFESEY
                     RYVTFDDPTQGAFFDDDPKGFMKRHSGRVIFDEVQKAPKIFDYLKLAIDEDRGSYGHY
                     ILTGSSQFAFVKGITESLAGRIGMLSLLPFQYSEIPSPLRERQIVNGSYPELVMRKYD
                     GLGEWYSSYIANYLERDVRSLYNLGNLRDFRRLLMLLAARSAQELNMSELAGEVGVSV
                     NTIRSWISILEASYIIFLLPPYHRNLGKRIVKRPKIYFYDTGIICHLTGISDRKLLDR
                     GPLAGPVFENYIVSEIKKIILHNGRESELYYFRNNLGLEADCIIEDRHAQTVHYVEIK
                     NTRTAKPRMADSLVKLIDLEKNEISPSTRSIEGRIVYAGEERTALSDSIQFIDYRDLL
                     EQLSHPAGT"
     gene            2133..3407
                     /locus_tag="PKJ16_10050"
     CDS             2133..3407
                     /locus_tag="PKJ16_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47373.1"
                     /translation="MKIPPAISTHIMVAMLIATIHAPLPLHAVEEKQPYRHNLVLGFD
                     RPYSVAEVNNATPLFLYRYFQEELPHDLYAELSILTTHVITVFGRKTREYFVGVSPQL
                     NHTVYSGFHAYTNGVSDYRRRFQGHNAGVEAFFQYHFIKPVSLKLSYYPGWYFYRENV
                     GRDIMQNHYEPTEIALPNNHWEHKGRADLVADFVTRRGLLDRIRHGVYARAIYEYARR
                     YGYGTFEDLIPETMKSSISHTHKSYAELGLYYSFSNDITLLGAYHHNIDRNNSDKIGA
                     FFSETAPMPGYFWGEFYHNKFATARAQIGLPLGVWHSRLQPGYNLLYLHKKNEVIGVG
                     NYAHTVYHSVSASFTTKLGDVVPMMLNYAYGINAVRRDAHTRRLEKGNHEVLVMVIAA
                     FGENPRPDGEPKEKERRTIPDELEGPEKMRVE"
     gene            complement(3593..3982)
                     /locus_tag="PKJ16_10055"
     CDS             complement(3593..3982)
                     /locus_tag="PKJ16_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47374.1"
                     /translation="MMNGRDDNVILDMSVPVGSTREGIIDGIVRALAQAGVRTLYDDS
                     LPDLLERLLSEVLRGGASDAIERGLFTHARRNEGYVEITVRDGAERNRCYSYLFDSKL
                     SAMTYAANGGAEDPMGVVVRVNMALRN"
     gene            complement(4177..4320)
                     /locus_tag="PKJ16_10060"
     CDS             complement(4177..4320)
                     /locus_tag="PKJ16_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47375.1"
                     /translation="MNYRIEKKTATENSNTENRGGFHLDVPRYIAGACAMIALLLIAK
                     YYL"
     gene            4750..4872
                     /locus_tag="PKJ16_10065"
     CDS             4750..4872
                     /locus_tag="PKJ16_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47376.1"
                     /translation="MSERTQKNVEAGTDARVDFKKIIIGGILLAGLLAFVKFFL"
     gene            4886..5443
                     /locus_tag="PKJ16_10070"
     CDS             4886..5443
                     /locus_tag="PKJ16_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HNV47377.1"
                     /translation="MLLGAILKSIRRDKEYTLGDVARRLDISTSLLSQIENDRVQPSL
                     HTLSTLLALYETPLSDFFRQAEHKRYQFISRLDSERLTHHGEGFSLTLLASKLQNNSL
                     ESFIVELRPGGAIDIAELSREYRGERMIYVIAGAPSVTIDNEGPFALEGGDSINFKCH
                     TPCAIANPSGSDARFVITGIPPIFL"
     gene            complement(5456..5869)
                     /locus_tag="PKJ16_10075"
     CDS             complement(5456..5869)
                     /locus_tag="PKJ16_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNV47378.1"
                     /translation="MQSQIMQSLSGILAFLAYFGGAIGLLVVFAGVYGLVTPYSEHTL
                     IREGKTAPAVSLGGALLGFACPLASAIAHSVSLLDMIIWALIALVIQVLAFIVLRLFF
                     GQVIKGIAEDKMGPAAFVAFVSVVVGLLNAASMTN"
     gene            complement(5871..6995)
                     /locus_tag="PKJ16_10080"
     CDS             complement(5871..6995)
                     /locus_tag="PKJ16_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HNV47379.1"
                     /translation="MRRIAITPRPDWLEKVESVGLTYHTINDAIYWDESAYYAFTSSE
                     VDTLDDATAELHGMCLKAVEHVIANKLFSRMAIPEGFVPLILRSWEDADPSMYGRFDF
                     CYDGTGDPKLLEYNADTPTALLEGSVVQWFWLKDLFPSKDQFNSIHEKLLDFWTAHKY
                     AGTVYFACMKDTEEDLGNIEYMRDVAMQAGLRTKQVFIEDIGWLPDRKQFVDLDNESI
                     FIIFKLYPWEWMFTDEFGSNLMECDWTVIEPAWKAILSNKAILPILWELYPDHPNLLP
                     AFFENTFSDNYVRKPFFSREGDSVLMVRGGETLERPGTYGKEGHIFQQYHPVPVFDGN
                     YVSIGSWIIDGKPAGIGIREDNTEITMNTSRFIPHLFEEE"
     gene            complement(7000..7341)
                     /locus_tag="PKJ16_10085"
     CDS             complement(7000..7341)
                     /locus_tag="PKJ16_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47380.1"
                     /translation="MSKLEEKEKRSSLRISLVLIGSVAFASCGEDGTRNVYNSQSDCL
                     AENSASDCEAVSSDSSDYSHSHRRYYGAFIPYRGGSSISSLRTGSKSVSVARATRGGF
                     GGSSGFHSSGS"
     gene            7487..8371
                     /locus_tag="PKJ16_10090"
     CDS             7487..8371
                     /locus_tag="PKJ16_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HNV47381.1"
                     /translation="MIQLAVNYSRPAAALSRAGRIAFDRFKCPAWPDVVAESLAIGPS
                     YVHYPILVGGARPVIDKETGADPDWGRIESMARDTGVPYINIHLAPTNADHPEAVDAD
                     GDPDAVRRFTDYFIDCVGRVVSRFGPARVILENNSDDWGQSLTIGILPGVIAEVVAAT
                     GCGFLLDISHARLAAERLGWDARDYTEALPVGRISELHVTGVQRFDESWIRPLREAGI
                     PEAQYREFIGHRIDHLPLTDDCWEFYEWSIGRIRSGAWSEPWAVAYECGGVSPLWEAL
                     TDADVLAEQVPRLLAALR"
     gene            complement(8375..8833)
                     /locus_tag="PKJ16_10095"
     CDS             complement(8375..8833)
                     /locus_tag="PKJ16_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47382.1"
                     /translation="MMRIIPLAAATCAVLLMGTAGFPHKPYPIDDERAVREAHAIVIA
                     TLRSSSKKNERCATTTVDRLRVESVIRGEVAVGTELSFVRSIFHWRRARWPWEKDCPS
                     VHYFVPPRVERASQGDRVIATLARDRPDGPLRVTGTIDISRLDAVRRLVR"
     gene            complement(8951..9613)
                     /locus_tag="PKJ16_10100"
     CDS             complement(8951..9613)
                     /locus_tag="PKJ16_10100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HNV47383.1"
                     /translation="MAGAERRRRRSIAMQLDIIVVIMIGALAALALAAGMAYQRIVSA
                     RLEEARRLLMEGSIVDPETGASSRRFLLERFGQEFQKARRHGSTLACIAVGIDDFALL
                     EERFGRDACARLLRQFADICRASARLYDIVGRYERDEFVLLLPDTDAPGAAAVAERIR
                     RSIAGDLVINTGTCNIFVSMSAGVAALVPTDEGVDHLLRRVYEALAAARNSGKNRIVK
                     AT"
     gene            9741..10502
                     /locus_tag="PKJ16_10105"
     CDS             9741..10502
                     /locus_tag="PKJ16_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV47384.1"
                     /translation="MIKERNEDNIIIATFENGKYNTITTETLLQIRDLVKRVTEDDAL
                     KGIVFTGNGRTYCSGFDLPTFLGFKELKDAVAFFDLAEPVFMEVFMCPKPVIAAINGS
                     AMAGGLIISMAADHRIAKNHPKIQIGMTEIKIGLGLTVVQSALMRYGLDSEKRFRTVM
                     YGGERYSPEASLALGIVDELASEEELLPRAKQIVTSWIDNPARAFRLLKYNLRKPVYD
                     QMKHYLATENWQDGLNCMFDPQTRATLELVASMMK"
     gene            10866..>11642
                     /locus_tag="PKJ16_10110"
     CDS             10866..>11642
                     /locus_tag="PKJ16_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47385.1"
                     /translation="MKNIIARIMQPYADSSYELRGRVRAFIQLNMALVPILVVFIIIM
                     NILIQRGLFQTLNVLVGSILIVIGVSVLLTARGLFNPAIIVFIIVTLAGLGFNMRGTD
                     LTGSGARFIGASAALSLPILLCTLFSTRWLLVAATALAQAIMLAVMAGATSIPPEIKP
                     VVTGTLSIIIILTGVIGYIINRINDTARRLRMDDTLRERERHLAINESLLESLKEVSR
                     KLDDSSHQLSSNSALFSDNIQSQASSIEEITATIEEISSGS"
BASE COUNT         2449 a         3653 c         3282 g         2258 t
ORIGIN      
        1 tccccgagaa aaagacgcgc accatcattt tcccataata tcgtacatgt cgcaatgaac
       61 attctccagc ccgaccgcct gagcgatgaa gtgacctgtc catacatccc ggggaaccgg
      121 tgccggtacc gctatttttt cgcgaccgac gttacggccg acgagctcga acacctgctc
      181 gcgaccggct ggcggaagtt tggcccctac tacttcatgc ccgagtgcgg cgagtgccgc
      241 gagtgcgttc cggttcgcgt gctcgcccgg gagttcgcgc cctccgcgaa ccagcgcaag
      301 acgctcgcca aggcgcgcgc catcgagacg cgtttcgagc cgcttgcgtt ctcggaagaa
      361 attttcgaca tctactgcga ccactccatc aaccgcttcg gctccaatcc ctcgctggat
      421 gacttcctcc acaacttcta ctacccctcg tgcccggccc tgcagtcgga gtaccggctg
      481 ggcgattcgc tcatcgccgc gggtttcatc gaccagtcga gccggggatt gagcagcgtg
      541 tacttcatct accgaacggc gcatgggcgc tacagcctcg gcacggtgag cgcaatcagg
      601 gagatcgaac acgcgcgcga actcggcctc gactactact acctcgggta tcacgtcgcg
      661 cgcaaccact tcatggccta taagaaccgg ttcaggccgt accagcggtt cgattgggag
      721 aaaaagcaat gggcgcagcc cgaggagtga ccgtcatccc gaagtgacat aatcaccaac
      781 attaatatat atacagattt tacttgacac ttaaaatctg tataataaat tcagatcatg
      841 aaatacctgc accgcgacgc ggaaaagacc atcgcgaggt actgttcgct cttcccggca
      901 gtcgcgctca ccgggccgcg ccagtcggga aaatctacca cgctcaagaa gcttttcgaa
      961 tcggaatacc ggtatgtaac ctttgacgat ccgacccagg gcgccttctt cgatgacgac
     1021 ccgaagggat tcatgaaacg ccattccggg cgcgtcattt ttgacgaggt ccagaaggct
     1081 cccaagatat tcgattacct caagctggcc atcgacgagg accgcggctc ctacgggcac
     1141 tacatactca ccggctcgag ccagtttgcc ttcgtcaaag ggataaccga gtcgcttgcc
     1201 ggcaggatcg gcatgctttc cctcctgccg tttcagtaca gcgaaatccc ttcaccgctt
     1261 cgagaacggc agatcgttaa cggcagttat cccgagctgg ttatgcggaa atacgacggc
     1321 cttggagaat ggtattcatc gtatattgcc aactatctcg agcgggacgt acggtctctc
     1381 tacaacctgg ggaacctgcg ggatttccgg cggctcctga tgctcctcgc cgcgcggtcg
     1441 gcgcaggaac tcaacatgag cgaactcgcc ggtgaggtcg gcgtgagcgt gaatacgatc
     1501 cgaagctgga tctcgatact cgaagcgagt tatatcattt tcctgctccc gccttatcat
     1561 cggaacctgg gtaagcggat cgtcaagcgt cccaagatat atttttacga taccgggatc
     1621 atctgccacc tcacggggat atccgaccgg aaattgctcg accggggacc gttggcggga
     1681 cctgtttttg agaactatat tgtttccgag ataaaaaaaa tcatcctcca caacggccgg
     1741 gaaagcgagc tctactattt tcgaaacaac cttggccttg aggccgattg tatcatcgag
     1801 gatcgccacg cgcaaaccgt ccactatgtc gagatcaaga acacccgcac cgcgaaaccg
     1861 cgcatggccg attcgctggt aaaactcatc gacctggaaa agaacgagat ttccccttcg
     1921 acacgatcaa tcgaaggccg tatcgtctac gcgggcgaag aacgaaccgc cctttccgac
     1981 agcatccaat tcatcgacta tcgcgatctc ctggagcagc tctcgcatcc tgccggaacg
     2041 taacattgtt gttgtttttt ccgcactcct tccttacgtt aaccgcacgc gccgcaggca
     2101 atgacgtatt ccataccgaa aaggagatgc gcatgaagat ccccccggcc atcagcacgc
     2161 atatcatggt cgccatgttg atcgcgacga ttcacgcgcc gctcccgctc cacgccgtgg
     2221 aggagaagca gccctatcgg cacaacctgg tccttggatt cgaccggccg tatagcgtcg
     2281 cggaggtgaa caatgcgacg ccgctgtttc tgtaccggta tttccaggag gagctcccgc
     2341 acgatctcta cgccgagctc tccatactca cgacgcacgt gatcaccgtg ttcggccgca
     2401 agacgcggga gtattttgtc ggggtctcgc cccagctcaa tcacaccgtg tacagcggat
     2461 ttcacgccta caccaacggc gtgtccgatt atcggcggcg gttccagggg cataatgcgg
     2521 gcgtggaggc gttcttccag taccacttca tcaaaccggt gtcgctgaag ctgtcgtact
     2581 acccgggatg gtatttctac cgggaaaacg tcggcaggga catcatgcaa aaccactacg
     2641 agccgaccga gatcgccctg cccaacaacc actgggagca caagggccgc gccgatctcg
     2701 tcgccgattt cgtgacccgc cggggactgc tcgaccgcat tcggcacggc gtgtacgccc
     2761 gggcgatcta cgagtatgcg cggcgatacg gctacgggac attcgaggac ctcatccccg
     2821 agacgatgaa atcgagcata tcgcacacgc acaaatcgta cgccgagctg gggctatact
     2881 actcgttctc gaacgacatc accctgctcg gggcatacca ccacaatatc gaccggaaca
     2941 actccgacaa gatcggcgcc ttcttctcgg aaacggcccc gatgcccggc tacttctggg
     3001 gggagttcta tcacaataaa ttcgccacgg cccgcgccca gatcggcctt ccgcttggcg
     3061 tctggcattc gcggctgcag cccggctaca acctgctcta tctgcacaag aagaacgagg
     3121 tgattggcgt cgggaactat gcgcacaccg tctaccacag cgtctcggca tcgttcacga
     3181 ccaagctcgg ggacgtagtg cccatgatgc tcaactatgc atacggcatc aacgccgtgc
     3241 gacgcgacgc gcacacccga cgactcgaaa agggaaacca cgaggtgctc gtcatggtca
     3301 tcgcggcctt cggcgagaac ccgcgtccgg acggcgaacc gaaggagaaa gagcggcgga
     3361 cgatcccaga cgagctcgag ggaccggaga agatgcgcgt cgaatagcag gcaacgtcac
     3421 cgcgggtttt ccgcacaggc gccgtgtttg atgcggcggg ccgaccggaa cgcgccgccg
     3481 cacacgcgtc accatcgaca ccagggcctg gatggccgat cccggaaccg cgttcccatg
     3541 caccacgcgc accatgcgcc agccgggcgc ccggtgcagt gcgcatgcgg cgctagttcc
     3601 tgagcgccat gttcacccgg accacgacgc ccataggatc ttcggctccg ccattcgcgg
     3661 cataagtcat cgcgctcagc ttcgaatcga ataaatagct gtagcagcga ttgcgttcgg
     3721 cgccatcgcg aacggttatt tccacatagc cctcatttcg ccgcgcgtgg gtgaacaggc
     3781 cccgctcgat cgcgtctgac gcgccgccac ggagcacctc cgaaagcagt cgctcaagaa
     3841 ggtccggcag ggaatcatca tacagcgtcc gtacgcccgc ctgcgcgaga gcgcgcacga
     3901 tcccgtcgat gatcccctcg cgggtcgagc cgaccggaac gctcatgtcg agaatcacgt
     3961 tgtcgtcgcg tccgttcatc atcacgcacc tccctggcac ggtcgctcct gcgacggtgc
     4021 cgctccccat gagcatccaa aagggaaaat tcaacctctc cgggaaaccc gttgcggggc
     4081 ggataccccg gagaggttag cgctcctcct cagcttggcg acaatgaagt atgaagaagg
     4141 taaagacggg ttttcgcaac actgggcgta aaaagcctat aaataatatt tcgcgataag
     4201 caaaagggcg atcatggcgc atgcgccggc gatatagcgg ggtacgtcga gatggaaccc
     4261 gccccggttc tccgtgttgg agttttctgt tgccgttttc ttttcgattc gatagttcat
     4321 cgattaccct cctccaatat ccccattcaa ctataattat atcaaaatcc acaatacccg
     4381 acaagtaaaa ttttattata agttaaaata ttgattaaaa tattaattta ttttgataaa
     4441 ttcacttgaa atatattcac taaatatttt ttgatattaa atattttaca tagttcacgc
     4501 aaaagatgct tcaataccag gggagggatc agaaaaaaga agggattccg atcaaacaga
     4561 tgcctgcctg atggaatccc cgagaggttc tccatgaaac gtggtcgtga tatggaggtt
     4621 tcaacccatt ttcacgttaa tataatataa aattttgtca ataaatatta atcgaaaatg
     4681 aattatattt gccaattttt tttctacgat ttcttgtgta cacaaacatt tttatggagc
     4741 ggatatgcca tgagcgaacg gactcaaaaa aacgtagaag ccgggaccga cgcacgagtc
     4801 gattttaaaa agatcatcat cgggggtatc ctgctggcgg gattgctggc gttcgtgaag
     4861 ttctttctat aagtaggagc gccccatgct ccttggcgcc atcctgaaga gcatacgccg
     4921 ggacaaggag tataccctag gcgatgtcgc gcggcggctc gacatatcga caagcctgct
     4981 ttcgcaaatc gaaaatgacc gggtccaacc ctcgctgcac acgctctcga cgctgcttgc
     5041 gctctacgaa acgccgctct cggatttctt ccggcaggcc gagcacaagc ggtatcagtt
     5101 tatcagcagg ctcgattccg agcggctgac ccatcatggc gaaggttttt ccctgacgct
     5161 gctcgcttcg aagcttcaga acaactcgct ggaatcgttt atcgtagaac tccggcccgg
     5221 cggggcgatc gacatcgccg agctgtcgcg ggaataccgc ggcgagcgga tgatctatgt
     5281 tatcgccggc gcgccgtcgg tgaccatcga caacgagggc cccttcgccc tcgaaggcgg
     5341 ggactcgatc aacttcaagt gccacacccc gtgcgcgatc gccaatccgt ccggcagcga
     5401 tgcgcgcttc gtgattacgg gcatcccgcc catcttcctg taagcgcggg gggcgtcagt
     5461 tcgtcatgct ggcggcgttc aggagcccca cgaccaccga tacgaacgcg acgaaggccg
     5521 ccggccccat cttgtcctcg gcgatccctt tgatcacctg gccgaaaaag agccgcagga
     5581 cgatgaacgc cagcacctgg atcaccagcg cgatgagcgc ccagatgatc atgtccagca
     5641 ggctcacgct gtgcgcgatg gcgctcgcca gcgggcaggc gaacccgaga agcgcgccgc
     5701 ccaagctcac tgccggcgcc gttttcccct cgcggatgag cgtgtgctcg gaatacgggg
     5761 tcacgagccc gtataccccg gcaaagacga ccaggagccc gatggcccct ccgaaatacg
     5821 caagaaacgc gagaatgccc gacaacgact gcatgatctg cgattgcatg ttactcctcc
     5881 tcgaacagat gcggtataaa ccggctggta ttcatggtaa tctcggtatt gtcctccctg
     5941 atcccgatgc ccgccggctt tccgtctatg atccatgagc cgatcgagac gtagttgccg
     6001 tcgaacacgg gaacggggtg gtattgctgg aagatatggc cctccttccc ataggtgccg
     6061 ggacgctcga gcgtctcgcc gccccgcacc atcagaacgc tgtccccctc gcgcgagaag
     6121 aagggtttgc ggacataatt gtcggagaac gtgttctcaa aaaaggccgg aagcagatta
     6181 ggatggtccg ggtagagctc ccataggatc gggaggatgg ccttgttgct cagaatcgcc
     6241 ttccacgcgg gctcgatgac ggtccagtcg cattccatga ggttcgagcc gaactcgtcg
     6301 gtgaacatcc actcccaggg ataaagcttg aagatgatga agatggattc gttgtcgagg
     6361 tctacgaact gcttccggtc gggaagccag ccgatgtcct cgatgaaaac ctgcttcgtg
     6421 cgcaggcccg cctgcatcgc cacgtcgcgc atgtattcga tgttccccag gtcctcctcg
     6481 gtgtccttca tgcaggcgaa gtacacggtc cccgcgtatt tatgcgccgt ccagaagtcc
     6541 aagagcttct cgtggatgga attgaactgg tccttcgacg ggaagaggtc cttgagccag
     6601 aaccactgca ccacgctccc ctcgagcagc gcggtgggcg tatcggcatt gtactcgaga
     6661 agcttgggat cgcccgttcc gtcgtagcag aagtcgaagc ggccgtacat cgagggatcg
     6721 gcgtcctccc acgagcgcag gataagcggc acgaagccct cggggatcgc catgcgcgag
     6781 aagagcttgt tcgcgatcac gtgctccacg gccttgaggc acatgccatg caactcggcc
     6841 gtcgcatcat cgagggtatc gacctcgctc gacgtgaagg catagtaggc cgactcgtcc
     6901 cagtagatgg cgtcattgat ggtatgatag gtgagcccca ccgactcgac tttttccaac
     6961 cagtcgggac ggggagtgat cgcgatccgg cgcacgccgt cagctcccgc tcgaatggaa
     7021 cccggaggac ccgccgaagc cgccccgggt cgcgcgcgcg acgctcacgg acttgctgcc
     7081 cgtacgcagc gacgaaatgg agcttccgcc tcggtagggg atgaacgcgc cgtaatagcg
     7141 gcgatgcgag tggctgtaat cggacgaatc gctcgaaacg gcctcgcagt cgctcgcgga
     7201 attctccgcc aggcagtcgc tctggctgtt gtagacgttg cgcgtgccgt cctcgccgca
     7261 actggcgaag gcgacggatc caatgaggac gagcgagatc ctgagactgc tccttttctc
     7321 tttctcttcg agttttgaca tgaggcctgc cctccgggtt ctttttctgt atgggtggat
     7381 tgttgatggg gacataattg atatcagaaa atattgcaag gaattttcgt tatccattga
     7441 cgaaaaccgc tgatcgccgt tactagaccc gcttcaacgg ggaggaatga tacagctcgc
     7501 agtcaactat tcccggccgg ccgcggcgct ctcccgcgcg gggaggatcg ccttcgatcg
     7561 cttcaagtgc cccgcatggc ccgatgtggt cgcggaatcg ctcgcgatcg ggccctccta
     7621 tgtccattac cccatcctcg tgggcggggc gcggcccgtg atcgacaagg aaaccggcgc
     7681 cgatccggac tggggccgga tcgaatcgat ggcgcgcgac accggcgtgc cgtacatcaa
     7741 catccacctc gccccgacaa acgctgacca tcccgaggcc gttgacgcgg acggcgatcc
     7801 cgacgcggtt cggcggttca ccgactactt catcgactgc gtgggccggg tcgtgtcgcg
     7861 cttcggaccc gcgcgcgtca tcctggagaa taactccgac gactggggtc agagcctcac
     7921 gatcggcatc ctgccggggg tgatcgccga ggtggtcgcg gcgaccggct gcgggttcct
     7981 tctggacatc tcgcacgcgc gcctcgcggc ggagcgtctg gggtgggacg cgcgcgacta
     8041 caccgaggcg ctcccggtgg gacgcatcag cgagctccac gtgaccggcg tccagcgctt
     8101 cgacgaaagc tggatacggc cgctgcgcga ggccggcatt ccggaggcgc aataccgcga
     8161 gttcatcggc caccggatcg accacctgcc cctcacggac gactgctggg aattctacga
     8221 gtggtcaatc ggccggatac gaagcggtgc gtggagcgag ccctgggcgg tcgcctacga
     8281 atgcgggggc gtgagccccc tctgggaggc gctcaccgat gcggacgtgc tcgccgagca
     8341 ggtcccgcgc cttctcgcgg cgctgcgtta ggcgctatcg aacaagacgc cgcacggcgt
     8401 cgagccggga gatgtcgatg gtgcccgtca cgcgcagcgg cccgtccggc ctgtctcgcg
     8461 cgagcgtcgc aatcacccga tcgccctgcg aggcgcgctc gacccggggc gggacaaaat
     8521 agtgcacgct ggggcagtcc ttctcccagg gccagcgcgc cctccgccag tgaaagatcg
     8581 agcgcacgaa cgaaagctcc gtccccacgg cgacctcccc gcggatcacg ctctccacgc
     8641 gcaagcgatc gacggtcgtg gtcgcgcacc gctcgttctt tttcgatgat gaacggagcg
     8701 tcgcgataac gatcgcatgg gcctcgcgca cggcgcgctc atcgtcgatg ggatacggct
     8761 tgtgcgggaa accggccgtc cccatgagga ggaccgcgca tgtcgctgcc gccagcggaa
     8821 ttattcgcat catcgcgtcc tcatcgtaga atttatatcg acatgccgca tccgtattca
     8881 tttttgtatg atccgcccga ggcggcgcaa cggctcaatc ggtccgcgcc gggccaggcg
     8941 gtcgagcgcc tcacgtcgcc ttcacgatgc ggttcttccc gctgttgcgc gccgcggcga
     9001 gggcctcgta cacgcggcgg aggaggtgat cgacgccctc gtccgtcgga acgagggccg
     9061 cgacgccggc gctcatgctc acgaagatat tgcacgtccc ggtgttgatg acgagatccc
     9121 ctgcgatgct ccgccggatg cgctcggcga cggccgcggc gcccggcgcg tcggtgtcgg
     9181 ggagcagcag aacaaactcg tcccgctcgt aacggcccac gatatcgtaa agccgggcgc
     9241 tcgcgcggca gatatcggcg aactgccgga gaaggcgcgc gcaggcatcg cgcccgaagc
     9301 gctcctcgag gagcgcgaaa tcgtcaatgc caacggcgat gcaggcaagg gtgcttccgt
     9361 ggcgccgggc cttctggaac tcctgcccga agcgctcgag gagaaagcgc cgtgagctcg
     9421 ccccggtttc gggatcgacg atggagccct ccatgagaag ccgccgagcc tcctcgagcc
     9481 gggcgctcac gatccgctga taggccatgc ccgcggcaag ggccagtgcc gccaaagcgc
     9541 caatcatgat gacaacaatg atatcgagct gcatcgcaat cgagcggcgc cgcctgcgct
     9601 ccgctcctgc catcacaata cgccccggga gcgtccgcgt caataaaaat gatagtgcga
     9661 cgggtgcaga agcgattgac ggatcaccgc cgatcgtgca tcacggtacc cgcactcata
     9721 tcagccacaa ggagaaccgc atgatcaagg aacggaacga ggacaacatc atcatcgcga
     9781 cattcgagaa cgggaagtac aacaccatca ccacggagac gctgctgcag atccgcgacc
     9841 tggtgaagcg cgtgaccgag gacgacgcgc tcaaaggcat cgtcttcacc ggcaacggac
     9901 gcacctattg ctcgggcttc gacctgccca ccttcctggg cttcaaggag ctcaaggacg
     9961 cggtcgcctt cttcgacctg gccgagccgg tgttcatgga ggtgttcatg tgcccgaagc
    10021 cggtcatcgc cgcgatcaac ggctcggcga tggcgggggg cctcatcatc tccatggcgg
    10081 ccgaccaccg gatcgccaag aaccacccga agatccagat cggcatgacc gagatcaaga
    10141 tcggcctggg gctcacggtg gtgcagtcgg cgctcatgcg ctacggcctc gacagcgaga
    10201 agcgcttccg caccgtgatg tacggcggcg agcgctacag ccccgaggcc tcgcttgcgc
    10261 tgggcatcgt ggacgagctc gcgagcgagg aggagctcct cccgcgcgcc aagcagatcg
    10321 tcaccagctg gatcgacaac cccgcgcggg ccttccggct gctcaagtat aatctccgca
    10381 agccggtgta cgaccagatg aagcattatc ttgcgaccga aaactggcag gatgggctca
    10441 actgcatgtt cgatccgcag acgcgcgcca cactggagct cgtcgcgagc atgatgaaat
    10501 aagacctcac tcccccaccc tctcccgcgg gagagggtgg gcgttttcaa tcgtcaatcg
    10561 cgaacatccg tcgcgcatct actcgccaaa ggcctattgc atctgccgac cacatccggc
    10621 tcgaggccga cctggtcctc gtctggggct ctgaccccgt ccttcccaag gctctaaggc
    10681 tctgacccca ctttcccggg gctctgaccc ctttaagcgc ctttcagggc aaaaaacgtc
    10741 attcgataat ttcacacccg ccggaaatga tattgttgac atgatttaac caaagaccca
    10801 ctattcatcc aatggggttt ttgaccattc cacctcacgg tgcgacacat acagaggcat
    10861 tatcaatgaa gaacatcata gctcgcatca tgcaacccta cgccgattcc tcctacgaac
    10921 tgcggggcag ggtcagagcc ttcattcagc tcaacatggc gcttgtcccg atcttggtcg
    10981 tcttcatcat aataatgaac atcctcatac agcgcgggct gttccagacg ctcaatgtcc
    11041 tggtgggctc catcctcatc gtcattggcg tctccgtgct gcttaccgca cgaggcctat
    11101 tcaaccccgc catcatcgtc ttcataatag tgaccctggc cggacttggg ttcaatatgc
    11161 ggggaaccga tctcaccggc tccggggcgc gttttatcgg cgcctcagcc gcattgtcgt
    11221 tgcccatctt gctttgcacg ctcttcagca cccgctggct ccttgtggcg gcgaccgccc
    11281 tcgcccaggc gatcatgctc gcggtcatgg cgggcgcgac atcgataccg ccggagatca
    11341 agccggtcgt cacgggaacg ttgtccatca tcatcattct caccggcgtc atcggctaca
    11401 tcatcaatcg catcaacgac acggcgcgcc ggctgcgcat ggatgatacg ctccgcgagc
    11461 gggagcgcca cctcgccatc aacgaaagcc tgctggaatc gctcaaggag gtatcgcgca
    11521 agctcgacga ttcctcgcac cagctctcgt cgaactcggc cctcttctcg gacaacatcc
    11581 agagccaggc gtcctcgatc gaggagatca ccgcgacgat cgaggagatc tcctccggct
    11641 cc
//
LOCUS       DAORHF010000106        11396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4859_length_11396_cov_6.327749, whole genome shotgun sequence.
ACCESSION   DAORHF010000106 DAORHF010000000
VERSION     DAORHF010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11396
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4859_length_11396_cov_6.327749"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKJ16_10115"
     CDS             <1..228
                     /locus_tag="PKJ16_10115"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175067.1"
                     /note="converts 2-oxoglutarate to glutamate; in
                     Escherichia coli this enzyme plays a role in glutamate
                     synthesis when the cell is under energy restriction; uses
                     NADPH; forms a homohexamer; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HNV47386.1"
                     /translation="AGGVSTSGLEMSQNSMRLSWPRDEVDKRLQQIMRSIHTAALDAS
                     EAYGKKGDYVTGANVAGFIKVAKAMHAYGIV"
     gene            435..734
                     /locus_tag="PKJ16_10120"
     CDS             435..734
                     /locus_tag="PKJ16_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47387.1"
                     /translation="MVTADIGDVIRITIEADEIDFDNFKPIQERIHRILRAHPRDIVI
                     NMERVDFIDSAGIAMLLKVIESVDVEKRSIDFVNFTDNVRNTLTVVNLSRIFKIV"
     gene            complement(781..1032)
                     /locus_tag="PKJ16_10125"
     CDS             complement(781..1032)
                     /locus_tag="PKJ16_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47388.1"
                     /translation="MGDYVVDLSVLILNTGTVDLTSANYLGLVKFPDSPYHYLGDLKN
                     ADRGPEAAVRYYRAAAQEFAESGWSPLVGRHLPEPSRAP"
     gene            complement(999..1466)
                     /locus_tag="PKJ16_10130"
     CDS             complement(999..1466)
                     /locus_tag="PKJ16_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47389.1"
                     /translation="MKKFLPVLLIAFAGCLTLPAHLKYPSKAGNSVLGVSITLRAPIA
                     IFSDHPSAVYFAKIEKGETHRTAKRIVAANFVARGRAYLVDCEPGEYVAVAAYTRKQI
                     VKHKKDYSKPDMTPAEYEYYCALFGERIVAASRVSVGKTISFLWATTSWTSPC"
     gene            complement(1516..2145)
                     /locus_tag="PKJ16_10135"
     CDS             complement(1516..2145)
                     /locus_tag="PKJ16_10135"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017718510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47390.1"
                     /translation="MPSRRTIIASASEDRLEKLIEERLRPGADKKTIDERIWDLFGED
                     WCVMFTDLSGFSRGVAKFGIIHFLQTIYESERLLIPIIEDNDGILLKVEGDSFLVIFR
                     NVKKALQCCISMQRTLKEYNENKPDEEKVLLCVGLGFGPMLRIGDTDVFGAEVNAASK
                     LGEDTAEAWEVLVTKAVRDRAKDMPDISFEEIAEVPPGAEQAFRVKYRL"
     gene            2273..2791
                     /locus_tag="PKJ16_10140"
     CDS             2273..2791
                     /locus_tag="PKJ16_10140"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV47391.1"
                     /translation="MKNSEIALERYRRLIAHVEAYEAALYAKYAPHIACTAGCDSCCI
                     LESVCAIEGFRMHEALRASGPAILSLVAGNRDRADGRCVLLLDGRCLLYAHRPIICRT
                     HGYPLLIEGRVDFCRRNFTELSTIDSENIISLDALNEALARINILFMEEVDDARFRTD
                     RLFLRELIAPMS"
     gene            2949..4289
                     /gene="larA"
                     /locus_tag="PKJ16_10145"
     CDS             2949..4289
                     /gene="larA"
                     /locus_tag="PKJ16_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HNV47392.1"
                     /translation="MRIELPWGPGALHLETPDTWTVHFPRLIQSPKAPPRDELKPVRD
                     ALRRPVGARALSALTLKNKNILIVVDDNTRPTPTHTFFHLVIDELKKAGAEEKRMTIL
                     TTLGIHTPMTEAEMRQKVGASNLAKIEWENHDAFNLERLHRFGYTSLGTPVILNQRIL
                     YSDIIVSVGMIEPHLWAGFGGGLKNILPGIAAADCIAIHHGVIAEPPYRYNSVGMLPE
                     ENPFRTDLEEIVRHISIPIFCLNVLIDHQRRIVDAVAGDPIASHRAGVEKCIARSGLA
                     IERQVDGIIVCSNPMDINFKQSMKGVGNSLPALRPGGVVMGFLRAERGMDDITLPDKS
                     KPLWLVKRLMRALGPARVMWLLDKVKTGLTVEERFLTYYSMQLMREHDLFFHVPTVSD
                     DEVRRMAFFENFPEAQAVIDRGARALGPDAHVAVFPEAGATFPIVGQRSPAERL"
     gene            complement(4397..4481)
                     /locus_tag="PKJ16_10150"
     tRNA            complement(4397..4481)
                     /locus_tag="PKJ16_10150"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4445..4447),aa:Leu,seq:cag)
     gene            complement(4530..7244)
                     /gene="greA"
                     /locus_tag="PKJ16_10155"
     CDS             complement(4530..7244)
                     /gene="greA"
                     /locus_tag="PKJ16_10155"
                     /inference="COORDINATES: protein motif:HMM:NF011309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HNV47393.1"
                     /translation="MDKENVLDKLNSIFREELWGRIEPKDVGISKFKILDDLFNGIVS
                     ADLINDTVDVCVAHLGEHADSINASYLLGLIGYHTGRMDDMVHLKKLIDLFLAHHKWA
                     VAEVIAEKILEYGENRMALKALATSLERMGRNKDAIPVWEDLLKIDRFDAEVAKKLAF
                     AIVEDDPNKSVYYMKLSIEGYIKNNDYHEILSLWNKLVSVSWEDVPFFERIERMLVDA
                     GQTDLAASLLKILFNKYRDDENPDQSIALLKKILTYTPGDTMARRDLVKFYEKKHHEN
                     SQYDLFLQLSKLNNFKCPVKHAIRDFENYIVFDRGNYVTHRAWGVGKIVEIDRERIVV
                     DFKDKPGHGMSIQMALTSLTPISSGHIAVMEYEDFESARAMFSEDIIGFFDVLVRSYG
                     GEVNVSEVKKDLVPRYIDEKGWSKWWTKVRTEIKKDPHFGFSEKKKNVIFMRDKPLTF
                     ADELLNRFTAAGSFSERLDAAFELINNVGAEDGGPVAPYFIDYFSDQVKGESHTKRIL
                     SYFILRGLVRFADEKKLRLDAVRAEVIDYIKGSGELPLISMKISSYDYKKDFVNLIME
                     SREDWTSISAEMLLETPVRIHKYIINNLIQSHAYTMLNGFIERVIRNAKQYPEIFIWA
                     AKNLYTRAWDYAWLDYSSESLTLSYFRLMNELKKIETKGNRLKNMMTETIFDNDQAVL
                     RYIVRLYDEPFLHKIYDLFRGVAYAEQSQIDKFLAAIRERYPDFKLTGAVAVEEDAES
                     DIEKVIVSQQGRDRMMAELNRMVSVDLVNISRELSLVADVSGDVRENVEYNALMEKQA
                     VLKMEINKLEAQMKKVEILNVATVSTETVAIGTVIAFEDAATGEPGRYTILGPWDSDY
                     EKRILSYQSPIARAFLGRAVGDEIVVKMGDVDRRYRIVAIERWGNGAQ"
     gene            complement(7403..8188)
                     /locus_tag="PKJ16_10160"
     CDS             complement(7403..8188)
                     /locus_tag="PKJ16_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNV47394.1"
                     /translation="MKKCYTFLLPVGLCAAVALVVPSVGYSKIVTNVKPDISAEQLKA
                     IVVEDFEATDQAKWTVDSTPKKLANPPKPEDNPVEKLEMKIIDGAPADMVPEKWASNN
                     KGTQNKKCIGVLFKFRYPGANSVHVIPKDGIELPGRSRAISLWFHSRGHDYTLEAWVE
                     DFQGNVHVLKFGSVDYVGWRPLKTYIPTNIPQKISTYPQTRFLKIKRFVIRAKPEALT
                     EDTYFFFDQIKVLTDDYEVNFDGEKLHEAFTKGGGAPAKDGAK"
     gene            complement(8216..8968)
                     /locus_tag="PKJ16_10165"
     CDS             complement(8216..8968)
                     /locus_tag="PKJ16_10165"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNV47395.1"
                     /translation="MKREVKLLVLFILTGFLIVLVGQRPTETFAQNQPADTKPAQETQ
                     PEGLVWDWLNDFENCEDWRAVSTCPLGDTKIRKIEGKPIKMEGGKVTPILKPEDLDEK
                     DKNLKHEDKYVLGVKTYFIDRGFDRVEVFPPNEYIIRGKAKEVRVWVLGRKFRHTLFM
                     KLRDFRGNIHKIKIGRLDFWGWREMKAIIPGWLPQSTRYAMLDKNLHFVSFFVESDTY
                     EVPGTFYFYMDNFRIRTDMSEFTGDTTIKDNW"
     gene            complement(9276..10274)
                     /locus_tag="PKJ16_10170"
     CDS             complement(9276..10274)
                     /locus_tag="PKJ16_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HNV47396.1"
                     /translation="MRVYATSRTAAPISAHIARCALCCALVAALCFFGEPGHARSTSR
                     EDHAWRDADDSGIRLSDTERSRRRDRRDRVRQTDRRNEIRGTERGAPPRSHADGRNDD
                     DERFAVPRYRDERPSLKRPASKKKAAPARSVSGSRAPAPNRGAIVHTVRRGEHLTGIA
                     RRYRVPAETIADANGIDTSESLRAGMRLQIPRARRAGASPSRETAVREHRGGQRFLWP
                     MNSVVRVQREQVDGVKSIGIVIVGRPGAPVVSASTGVVEKVGVMRGFGKYVVVRHGER
                     FITVYSQLDDIRVRRGERVPAGREIGSVDVRAGRLHFQINCDGKPCDPLAMLPRRG"
     gene            10613..10840
                     /locus_tag="PKJ16_10175"
     CDS             10613..10840
                     /locus_tag="PKJ16_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47397.1"
                     /translation="MENGRSESTETPTITRIHTGRFASKVFNLILAYKNSKIFQMINR
                     HQGLAIVLLVLWGMLIMGMFMWFVVSHALRQ"
     gene            10991..>11396
                     /locus_tag="PKJ16_10180"
     CDS             10991..>11396
                     /locus_tag="PKJ16_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47398.1"
                     /translation="MTAPFPIRAALAACAMIASIALSTRPVAGDPPASESWTPQGYQA
                     NLINIRSVNGKFYAYTVLLFNENPHFVNLIQADSVSLKMMERGGFHVSEYRNYLKTIS
                     EVKHRKLAESLLDVYLSQDKQAPKALRELERAL"
BASE COUNT         2259 a         3537 c         3228 g         2372 t
ORIGIN      
        1 gccggcggcg tatccacctc cggcctggag atgagccaga acagcatgcg cctgtcgtgg
       61 ccgcgcgacg aggtcgacaa gcgcctgcag caaatcatgc ggagcatcca cacggccgca
      121 ctggacgcat ccgaggcgta cggaaagaaa ggcgattacg tcacgggcgc gaatgtcgcc
      181 ggcttcatca aggtcgcaaa ggcgatgcac gcctacggca tcgtgtagcg cgtcattctc
      241 actgttccct gtacacgaca cggcgggccg cacgcccgcc gtgttttatt tccgttcccc
      301 acaccgcatt cggcattttc acttgacctg ccgcattgaa ctgatatatc gggcggagcg
      361 actcaaccat tcagtcgtac atatagatac ggacggggcc atcgcgtctc ccggtccggc
      421 caagaggtgc cccaatggtt acagcagaca ttggcgatgt cattcgaata acgatcgagg
      481 ccgatgagat cgacttcgac aatttcaagc cgatccagga gcggatccac cgcatcctgc
      541 gcgcgcaccc ccgcgacatc gtgatcaaca tggaacgggt cgatttcatc gattcggccg
      601 gcatcgcgat gctgctcaag gtgatcgaga gcgttgatgt cgaaaagcga tccatcgatt
      661 tcgtcaactt caccgacaat gtgcggaaca cgctcaccgt ggtgaacctc tcccggatat
      721 tcaagatcgt ataatcacgg ttccgggcat gtgcgatatg acccgagtcg acatccatgc
      781 tcacggtgcc cgcgagggtt ccggtagatg ccgcccgacg agcggcgacc agcccgactc
      841 cgcgaactcc tgtgccgccg cacgataata ccgcaccgcg gcctcgggac cgcgatcggc
      901 gttcttcagg tcgccgaggt agtggtaggg gctgtcgggg aatttgacca gccccagata
      961 attggcgctc gtcagatcca ccgtgccggt attgagaatc aacacggaga ggtccacgac
     1021 gtagtcgccc ataaaaacga aatcgttttt cccaccgaca cgcgggacgc ggcgacgatc
     1081 cgctcgccga acagcgcgca ataatactcg tactcggccg gtgtcatgtc gggcttgctg
     1141 tagtcttttt tatgtttgac gatctgcttt ctcgtgtaag cggcaaccgc gacatactcc
     1201 cccggctcgc aatcgacaag gtacgcgcgc ccacgagcca cgaagtttgc ggcaacaatt
     1261 cgcttggcgg tacgatgcgt ctcacccttt tcaatcttag cgaaatacac cgcgctcgga
     1321 tgatcggaaa aaatagcgat cggcgcgcga agcgtgatgg aaacgcccag gaccgagttt
     1381 cccgccttcg aggggtactt caggtgcgcg ggcagcgtga ggcacccggc gaacgcgatc
     1441 agcagcaccg gcaaaaattt tttcattgta tcggtccctc catttgtatg attcggcgcc
     1501 ggcgcgccgg cgccgttaca atcggtactt cacccggaag gcctgttcgg cccccggcgg
     1561 aacctcggcg atctcctcga aggagatgtc cggcatgtcc ttcgcccgat cgcgcaccgc
     1621 cttggtcacc agcacctccc aggcctcggc ggtatcctcc ccgagcttgc ttgccgcgtt
     1681 cacctccgcg ccgaacacgt cggtatcgcc gatccgcagc atgggcccga acccgagccc
     1741 cacgcacaag agcaccttct cctcgtcggg cttgttctca ttgtactcct tcagcgtccg
     1801 ctgcatgctg atgcagcact gcagggcctt cttgacgttt cggaaaatca ccaagaagct
     1861 gtcaccctcg accttgagga gaatgccgtc gttgtcctcg atgatgggaa tcaagagccg
     1921 ttccgattcg tagatggttt gcagaaaatg gatgatcccg aacttggcga ccccgcgcga
     1981 aaagccggag aggtcggtga acatgacgca ccagtcctcc ccgaagaggt cccagatccg
     2041 ctcgtcgatg gtctttttgt ccgcgccggg ccgcagccgc tcctcgatga gtttctcgag
     2101 gcggtcctcc gacgcgctgg caatgatggt tctcctgctc ggcatggcga tcctcctgta
     2161 tgccctcttt ctgtatgatc acggctgggg ccgcacgcta caccgtaaaa cggcttgtca
     2221 tgtgcgccac ggtcaattca atgcagacaa atgtcgaaac ggcaataaca aaatgaaaaa
     2281 cagtgaaatc gccctcgaac gctatcgcag gctcatcgcg cacgttgagg cgtacgaggc
     2341 ggcgctctac gcgaaatatg cgccgcacat cgcctgcacg gccgggtgcg attcgtgctg
     2401 catcctcgag tcggtgtgcg ccatcgaggg cttccgcatg cacgaggcgt tgcgggcgag
     2461 cggacccgcg atactgtcgc tcgtcgccgg caatcgcgac cgcgcggacg ggcgctgtgt
     2521 actcctgctc gacgggcgct gcctgctcta cgcgcaccgg ccgatcatct gccgcaccca
     2581 cggctatccg ctccttatcg aggggcgggt cgatttctgc cgcaggaact ttacggagct
     2641 gtcgacgatc gactccgaaa acatcatcag cctcgacgcg ctcaatgagg cccttgcccg
     2701 catcaatatc ctcttcatgg aggaggtcga tgatgcgcgc ttccgcacgg accggctttt
     2761 tcttcgcgaa ctcatcgccc ccatgtcgtg atgcgcgtcg catcggaaga aaaaaatttg
     2821 acaccgaaca tcgccgccgt ctacgatagt gccggtttca accagccgac catatacaaa
     2881 aagcggatcg ggtcgcgcgc aggatacacc ggcgatgcgc gcggacagcg accgatatga
     2941 ggttgtccat gaggatcgaa ctgccgtggg gccccggcgc ccttcatctc gaaacacccg
     3001 acacctggac cgttcacttc ccgcgcctga tccaatctcc caaagcgccg ccgcgcgacg
     3061 agctcaagcc cgtgcgcgat gcgctcagga ggcccgttgg cgcgcgggcg ctgtccgcgc
     3121 tcacgctcaa gaacaagaac attctcatcg tcgtcgatga caatacgcgg cccaccccca
     3181 cgcacacgtt tttccacctg gtcatcgacg aactcaagaa ggccggggcc gaggaaaagc
     3241 gcatgacgat cctcaccaca ctgggcatcc acacgccgat gaccgaggcc gagatgcgcc
     3301 agaaggtcgg cgcgtcgaac ctggcgaaaa tcgagtggga aaaccacgac gccttcaacc
     3361 tcgaacggct gcaccgcttc ggctatacct cgctgggaac gcccgtgatt ctcaatcagc
     3421 ggattttgta ttcggacatc atcgtcagcg tggggatgat cgagccgcac ctctgggcgg
     3481 gattcggcgg cgggcttaag aacatccttc ccggcatcgc cgcggccgac tgcatcgcga
     3541 tacatcatgg cgtgatcgcc gagccgccgt atcgctacaa tagcgtgggc atgctcccgg
     3601 aggagaatcc gtttcgcacc gacctcgagg agatcgtccg ccacatctcc atcccgatct
     3661 tctgcctcaa tgttctcatc gaccaccagc gccgcatcgt ggacgccgtc gccggcgatc
     3721 ccatcgcgtc ccaccgcgca ggcgtggaaa agtgcatcgc ccgctcgggc ctggcgatcg
     3781 agcggcaggt cgacggcatc atcgtgtgct cgaacccgat ggacatcaac ttcaaacaga
     3841 gcatgaaggg cgttggaaac tcgcttccgg cgctgcgccc cggcggcgtg gtgatgggtt
     3901 ttttacgcgc cgaacgcggg atggacgata taacgcttcc cgataaatcg aagccgctgt
     3961 ggctggtcaa gcggctcatg cgcgcgctgg gtcccgcgcg cgtgatgtgg ctgctcgaca
     4021 aagtcaagac gggcctcacc gtcgaggaac gcttcctgac ctattatagc atgcagctca
     4081 tgcgcgaaca cgatctcttc tttcacgtgc ccaccgtgag cgacgacgaa gttcggcgca
     4141 tggccttctt cgagaatttc cccgaggcgc aggcggtcat cgaccgagga gcgcgtgcgc
     4201 tgggtccgga tgcgcatgtt gccgtcttcc ccgaggccgg ggccacgttc cccatcgtgg
     4261 gacagcgctc gccggcggag cgcctgtgaa ccggagattt ccgcggtgtg aatgtggcgc
     4321 ggagccaatc atcgttacca acgaccggaa aataaaaaac gccgctgcgc atgcaacggc
     4381 gtttttcctt gtgctgtgcc gcgagaggga ctcgaaccct cacaggcgta acgcccgcta
     4441 gaacctgaat ctagtgcgtc taccaattcc gccatcgcgg ccctgaggca gtatcacaaa
     4501 cgggctcgca gcccattttg tcaaatattt tattgcgcgc cgtttcccca gcgttctatc
     4561 gcgacgattc gatagcgacg atcgacgtcg cccatcttca cgacgatctc gtcgccgacc
     4621 gctctgccga gaaacgccct cgcaatgggc gattgatacg aaaggatccg cttctcgtaa
     4681 tcggagtccc agggcccgag gatcgtgtag cgacccggtt cgcccgtggc ggcgtcctcg
     4741 aacgcgatca ccgtgccgat ggcgaccgtt tcggtggaga cggtcgcgac gttgaggatc
     4801 tcgaccttct tcatctgcgc ctcgagcttg ttgatctcca tcttgagcac ggcctgtttc
     4861 tccatcagcg cattgtactc cacgttctcc cggacatcgc ccgatacgtc cgcgaccagc
     4921 gacagctcgc gcgagatgtt caccaggtcc acgctcacca tgcggttgag ctcggccatc
     4981 atccggtcgc gcccctgctg cgatacgatc accttctcga tatcactctc ggcgtcctcc
     5041 tcgaccgcga ccgcacccgt gagcttgaag tcgggatagc gctcccgaat cgccgcgaga
     5101 aacttgtcga tctgcgactg ctccgcgtag gccaccccgc gaaaaagatc gtagatcttg
     5161 tgcaggaagg gctcatcgta caggcggacg atataccgga gcaccgcctg gtcgttgtcg
     5221 aaaatcgtct cggtcatcat gttcttcagg cggttgccct tggtctcgat cttcttgagc
     5281 tcgttcatga gccgaaaata ggacagcgtg aggctctcgc tcgaatagtc cagccaggcg
     5341 tagtcccatg cgcgcgtgta caggtttttc gcggcccaga tgaagatttc cgggtattgc
     5401 ttggcgttgc gaatcacccg ctctatgaag ccgttgagca tggtgtaggc gtgcgactgg
     5461 atcaggttgt tgatgatgta cttgtggatc cgcacgggcg tctcgagcag catctcggcg
     5521 ctgatggacg tccagtcctc gcgcgactcc atgatgaggt tcacgaagtc cttcttgtag
     5581 tcgtaggaac tgatcttcat cgagatgagc ggcagctccc cgctcccctt gatgtagtcg
     5641 atcacctccg cgcgcacggc gtcgagccgc agcttcttct cgtcggcgaa ccgcaccagt
     5701 ccccgcagga taaaatacga caggatgcgc ttggtatgcg actccccctt cacctgatcg
     5761 gagaagtaat cgatgaagta cggcgcgacc ggtccgccgt cctccgcgcc gacattgttg
     5821 ataagctcaa aggccgcgtc gagccgctcg ctgaagctcc ccgccgccgt gaagcggttg
     5881 aggagctcgt cggcgaaggt gagcggcttg tcgcgcataa agatcacgtt cttcttcttc
     5941 tcggagaagc cgaagtgcgg gtccttttta atctcggtgc gcaccttggt ccaccatttc
     6001 gaccacccct tctcgtcgat gtagcggggc accaggtcct tcttcacctc gctcacgttc
     6061 acctcgccgc cgtacgagcg taccaacacg tcgaagaagc cgatgatgtc ctcggaaaac
     6121 atcgccctgg cggactcgaa gtcctcgtac tccatcacgg cgatgtggcc ggaactgatg
     6181 ggcgtcagcg aggtgagcgc catctgtatc gacatgccgt ggccgggctt gtccttgaaa
     6241 tcgacgacga tccgctcgcg gtcgatctcg acgatcttgc cgacgcccca cgcgcggtgc
     6301 gtgacgtagt tgccgcggtc gaagacgatg tagttttcga aatcgcgaat ggcgtgcttc
     6361 accgggcact tgaagttgtt gagcttcgac aattgcagaa acaggtcgta ctggctgttt
     6421 tcgtggtgct ttttttcata gaacttgacc aagtcgcgcc gcgccatggt gtcgccgggc
     6481 gtgtaggtga ggattttttt cagcagcgcg atggactgat ccgggttttc gtcgtcccga
     6541 tacttattga agaggatctt caggaggctg gccgcgaggt cggtctggcc cgcgtccacc
     6601 agcatgcgct cgatgcgctc gaagaagggc acgtcctccc acgatacgga caccagcttg
     6661 ttccacaggc tcaggatctc gtggtaatcg ttgttcttga tgtagccctc gatggagagc
     6721 ttcatgtaat agacgctctt gttcggatcg tcctcgacga tggcgaaggc gagctttttg
     6781 gcgacctcgg catcgaagcg gtcgatcttc aggaggtcct cccaaaccgg gatcgcgtcc
     6841 ttgttgcgcc ccatgcgctc cagtgacgtc gccagcgcct tgagggccat gcggttctcg
     6901 ccgtactcga gtatcttctc cgcgatcacc tccgcgaccg cccacttgtg gtgggcaagg
     6961 aataggtcga tgagtttctt gagatggacc atgtcgtcca tccggccggt gtgataccca
     7021 atgagcccca gcagatacga ggcgttgatg gaatcggcgt gctcgccgag atgggcgacg
     7081 cacacgtcaa cggtgtcgtt gatcaaatcg gcgctcacga tcccgttgaa caggtcgtcc
     7141 agtatcttga acttcgatat gcccacatcc ttgggttcga tgcggcccca gagctcttca
     7201 cgaaagatcg agttgagttt atccagtacg ttttccttat ccataataga accgcctcgt
     7261 ttccgtaagg atgaccgccc gggcatattt gggcggtttc aagaaacgaa aatacccacc
     7321 gtttcctgga tgggtatttt cagagtacag cgttgaaatc cctggttgcg tgtgctactg
     7381 ttgccgccgg tactccatga tattacttgg cgccgtcctt cgcgggagcg ccgccgccct
     7441 tggtaaaggc ctcgtgcagc ttctcgccgt cgaagttcac ttcgtaatcg tcggtgagaa
     7501 ccttgatctg atcgaagaag aaataggtgt cttcggtcag cgcctcgggc ttcgcccgaa
     7561 tcacgaagcg cttgatcttc aggaaccgcg tttgcggata ggtcgatatc ttctgcggaa
     7621 tattggtcgg gatataggtc ttcagcggac gccagccgac gtaatcgacc gaaccgaact
     7681 tgaggacgtg cacgttgcct tggaagtcct cgacccaggc ttcgagcgta taatcgtgcc
     7741 cgcgggaatg gaaccacagg gagatggccc gcgaccggcc gggaagctcg atcccgtcct
     7801 tcggaatgac atggaccgaa ttggccccgg gatacctgaa cttgaacaga acgccgatgc
     7861 atttcttgtt ctgggttccc ttgttgttcg acgcccattt ctcggggacc atgtcggcgg
     7921 gagcgccgtc gatgatcttc atttcgagct tctcgaccgg gttgtcctcc ggcttgggcg
     7981 gattggcgag tttcttgggc gtcgaatcga ccgtccactt ggcctggtcg gtcgcctcaa
     8041 agtcctccac gacgatcgcc ttgagctgct cggcgctgat gtcgggtttg acattcgtta
     8101 cgatcttcga atacccgacg ctcgggacca ccagcgcgac cgcggcgcac aggccgacgg
     8161 gcagcaggaa cgtataacac ttcttcatga gtatgcctcc cttcatctcc taattttacc
     8221 agttgtcctt gatggtcgta tcgccggtga actcggacat atcggtcctg atcctgaaat
     8281 tgtccatgta gaaatagaac gttccgggga cctcgtaggt gtcgctttcg acgaagaacg
     8341 atacgaagtg cagattctta tccagcatcg catagcgggt cgattgcggc agccagccgg
     8401 ggatgatggc cttcatctcg cgccagcccc agaaatcgag ccggccgatc ttgatcttgt
     8461 ggatgttgcc gcggaaatcg cgcagcttca tgaacagcgt atggcggaac ttacggccca
     8521 gcacccatac ccgcacctct ttcgccttgc cgcggatgat gtattcgttg ggcggaaaca
     8581 cctcgacgcg gtcgaagccg cgatcgatga aataggtctt cacgccaaga acgtatttat
     8641 cctcatgctt gaggttcttg tccttttcgt cgaggtcttc gggtttcagg atgggggtca
     8701 ccttgccgcc ttccatcttg atcggcttgc cctcgatctt gcggattttg gtatcgccaa
     8761 gcgggcaggt cgatacggcc cgccaatctt cacaattttc gaaatcgttg agccagtccc
     8821 agaccagccc ttccggctgc gtttcctggg cgggtttcgt gtccgcaggc tgattttgcg
     8881 cgaaggtctc ggtgggacgc tgcccgacga ggacgatcaa gaaaccggtg agtatgaaca
     8941 gtactagtag tttaacctcc cttttcatcc tcaactcctt ttcatcataa aagtttaaag
     9001 aaacagaatg tcactgtgct gatgtcacgc ccaaaaaccg gatagtgata tcaacgcccc
     9061 ccgctcacgg ctcgcgcacc gggaaacgct tattattgat tatcggccgg cacgatcaaa
     9121 acattaaaga aaaacaaatc cagccagacc ggtatttgta ttatatattt caacactgtc
     9181 aagaaattta tcagtcattt aacggtatat aatagtaata acaaaactaa tcatattaat
     9241 aaaaataatc accctttgaa aaccgcgccc gtccctcacc cgcggcgggg gagcatggcc
     9301 agcggatcgc agggcttgcc gtcacagttg atctggaagt gcagccggcc ggcgcgcaca
     9361 tccacgctcc cgatctcgcg cccggcggga acgcgctcgc cgcgccgcac ccggatatcg
     9421 tccagctgcg aatagaccgt gatgaagcgc tccccgtggc gcaccacgac atatttcccg
     9481 aacccccgca tgacgcccac cttctccacc acgcccgtcg atgcggaaac gaccggcgcg
     9541 cccggccggc ccacaatgac gatgccgatc gacttgacgc cgtcgacctg ctcgcgctgg
     9601 acgcgcacga ccgagttcat cggccacaaa aagcgctggc cgccgcgatg ttcccgaacg
     9661 gccgtctcgc gtgacggcga tgcccccgca cggcgcgcgc ggggaatctg caatcgcatg
     9721 ccggcgcgca acgactcgct cgtgtcgatc ccgttggcgt cggcgattgt ttccgcggga
     9781 acgcgatagc ggcgggcgat gccggtgagg tgctccccgc ggcgaaccgt gtgcacgatg
     9841 gcgcccctgt tcggcgccgg cgcgcgagaa cccgacaccg agcgggcggg cgccgccttt
     9901 tttttgctcg cgggccgctt cagggagggc cgctcgtctc ggtagcgcgg gaccgcgaag
     9961 cgttcgtcat cgtcgtttcg cccgtcagcg tgcgatcgcg gcggcgctcc gcgttcggtc
    10021 cctcggatct cgttgcggcg gtccgtctgt cgcacacggt cgcgccggtc acgccgccgg
    10081 cttcgctcgg tgtccgagag ccggatcccc gaatcgtcgg catcccgcca ggcgtggtcc
    10141 tcgcgcgaag tgctccgggc atggccgggt tccccgaaga aacaaagggc cgctaccagc
    10201 gcgcagcaga gcgcgcagcg cgcgatgtgc gccgatatcg gtgcagccgt tctcgatgtg
    10261 gcgtagacac gcatggtgaa acatccctgt tgttcgcgga acacatccgt atgttccggt
    10321 aatcgccgtc gggccgcacc gaaccggtgc ggtccccgga aaaggcgggc aagaggtttt
    10381 tcacggcacc accggcggac aatccgacgg aatcagcccg agcatcacaa aaaatacatt
    10441 ttgtccactc tcgttctcaa ccgcgcactt ccccgtattc ttactatcgg aagtggagac
    10501 agtatggttt agcataattt atttttttct cgttgaatat tggattgaca atacgcggaa
    10561 ttttcacacc atcctgcgca cagagaaaga tcgagggggt cttcactaca acatggaaaa
    10621 cggacgcagc gaaagcaccg aaacaccgac aataacccgc attcacacgg gacggttcgc
    10681 gtcgaaggtc ttcaacctga ttctcgcata caagaattcg aaaatctttc agatgatcaa
    10741 ccgtcaccag ggcctcgcca tcgtcctcct ggtgctctgg ggcatgctca tcatgggcat
    10801 gttcatgtgg ttcgtcgtct cgcatgcgct tcgccagtaa cgcccgcacc actcctcacg
    10861 gacatcgcag cggtcgacat acgccccgcg ccatcggcga cgcgacgttg ggcgggcgaa
    10921 ttaacattga aaaatccctt gcgcgcgaaa tattgaatga gtacatacgg cgtagtagaa
    10981 tgaacctggt atgactgcac cattccccat ccgagcggcg ctcgccgcgt gtgcgatgat
    11041 cgcgtcgatc gcgctgtcca cgcgtcccgt cgcgggggac ccgcccgcat cggagagctg
    11101 gacgccgcag ggctatcagg ccaacctcat caacatccgc agcgtgaacg ggaagtttta
    11161 cgcctacacc gtgctgctgt tcaacgagaa cccgcacttc gtcaacctga tccaggccga
    11221 cagcgtgtcg ctgaagatga tggagcgcgg aggctttcac gtgagcgaat accgcaacta
    11281 cctcaagacc atctccgagg tcaagcaccg taaactcgcg gaatccctgc tcgacgtgta
    11341 tctgtcgcag gacaagcagg ccccgaaagc gctccgggag ctcgagcgcg ccctcg
//
LOCUS       DAORHF010000107        11356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4893_length_11356_cov_5.539864, whole genome shotgun sequence.
ACCESSION   DAORHF010000107 DAORHF010000000
VERSION     DAORHF010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4893_length_11356_cov_5.539864"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="PKJ16_10185"
     CDS             complement(<1..1975)
                     /locus_tag="PKJ16_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV47399.1"
                     /translation="MYESLFTPIRINTLEVRNRIAYPSLGLLYSYDGTLNQRYHDFYV
                     ERARGGAGIVTIGPVGIDFIGSGGVPISLDNDDAIPQFKRLADDIRSHGASPWVQLYH
                     AGAYAFSFLLKGEKPVAPSAVYSKFKKETPRELGIDEIKQLQEAFVRAAERAKEAGID
                     GVEIIGSAGYLITQFLSPVTNKRTDEYGGSLENRLRFPVEIIQSMRARLGASYPLGIR
                     MAGNDFVPGSNTDRETPLFAQAYERAGIDVINVTGGWHETRVPQITREMPRGGFAYLA
                     LNIKRAVSVPVMASNRIPDPDTAERIIRDGVADMVNLGRVLIADPEWPNKVREGRAAE
                     VRPCVACGQGCMDELFSARPVMCIANPMAGFEGERAIIPTNHPKKIMVIGAGPGGLEA
                     AVTASQRGHAVTLYEKSGDIGGQLHIAGAPPHKQELWEIIRYYRAMLRALPIALHLNT
                     EATAETVAAERPDHVIVAEGAEPFVPKIDGIDGPGVVSAWTVLREDPMIGRRVAVIGG
                     GAVGIETAQFIAAKGTISPEVLEFLFLRDAEPVERIRELATRGSKEVTIFEMLPKIGK
                     DIGRSTRWVVMADVERDGITVIKNAKVLSVKDGKVTYEHNGATESVQFDTVVTAIGHP
                     EAIAEAAHRGIRKAGEVIDMAQHKGAHPRQGSTD"
     gene            2254..3012
                     /locus_tag="PKJ16_10190"
     CDS             2254..3012
                     /locus_tag="PKJ16_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV47400.1"
                     /translation="MDYKTIQFDIIEKGIGHLRLNRPEAYNAVNHLMMEELDAFWRER
                     LYDLDTVVIILSGNGEKGFCAGLDLKDTAEMAPKMNVAEFYRFQARLARINLAMRRAP
                     QPIICTVHGAAAGQGFSFSLASDIRIISPDARFNAAYINIGLGGADMSCSYFLPRMIG
                     AGRAYEFMYTGMFMSAQEAQALGMVSRVVERDKLHETALEIARVMMTKNHMGLRLTKE
                     AVNMNLDANGLEHALNMEDRNQTLLAIGTFMGKK"
     gene            3199..3666
                     /locus_tag="PKJ16_10195"
     CDS             3199..3666
                     /locus_tag="PKJ16_10195"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV47401.1"
                     /translation="MTVVEMPSRGLSEYFGGGIDVMVKKAFLGIGVLLFIAGGFFIFK
                     TVSFMNNSNKVDAKVVSVQTKVHKGKKMSRTTTYKPTFEFTDAGGKSVTATSFETSKS
                     YNFPIGSSVEILYDKNDSTNIKIPTFFSLWLIPTILIPIGLIFFIVGLVGKRK"
     gene            3744..5006
                     /locus_tag="PKJ16_10200"
     CDS             3744..5006
                     /locus_tag="PKJ16_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNV47402.1"
                     /translation="MKRKSLLVCVLFLVVSQGLFGGNTADELYRKAVALEKKGRYGQA
                     AQTMNEVLAAVPGNDFYLAYASHVERLAGDYENGLRHAITAISLNGAVGWYHASAMCN
                     AWGCADIDATRTYARKVVSLGPTAAGKENFDYAQTLLKSLSRRVYTITWRLDPSKGVK
                     TDGRYRVPLPTDGLPYQRARFDVEGCSERTVVVAEGNDVLLFSSAGGGVVTIRAEIAI
                     TPFSFRKRLSGHAARGDCRVPDDAAPYLGRSDRVDPNSAAVRMVAGPLKHREPMVSVR
                     NILSWMNKNIKYEIKDFRHVDEILRRRNAECGGWSALFTALCRANGIPAREVWGVIED
                     PTPDRRFAPAGHLKGHAWAEFYLCGIGWVPVEPQRPDTLGFLPATYVRMYHYDVVSSL
                     TSTHAYRPNGNMVLMNGDTPAFVVDVKE"
     gene            5491..5859
                     /locus_tag="PKJ16_10205"
     CDS             5491..5859
                     /locus_tag="PKJ16_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47403.1"
                     /translation="MPLPHNIPLVDTSREKGALVEVYAACSGKSCNTRIRSDGKFAVR
                     LDKNNTRPEIYDAFGKAAQKASGDSALMRGKSPVFIELIHNLHTAQAQSLRNGIIVYL
                     GLLALWFAVSIWSIRADAKS"
     gene            5899..6429
                     /locus_tag="PKJ16_10210"
     CDS             5899..6429
                     /locus_tag="PKJ16_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47404.1"
                     /translation="MRMQGLEHLIRQTYPNVNIAKTKRLGKMRIRMSRPGAMSWQKFK
                     NVDMVETDHAYFFDPREGLIKSYKRQLLIWSVLLPLSVILVVNGLFPAVIVMVISALG
                     VIACPINLAMSRKPKRIIMIWKNRIRTNDVVDGEHILQGALMKGDGFSIVADMKEVSD
                     GGTIGYDADFIIRCTV"
     gene            complement(6441..7448)
                     /locus_tag="PKJ16_10215"
     CDS             complement(6441..7448)
                     /locus_tag="PKJ16_10215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV47405.1"
                     /translation="MRHNHIARTVLLAILTVSNTAAAPGVRTASPPAKVPPGYQVPAA
                     KDLKWDRDRVTVKLTSRGFSQGDIVYVEITPRERAIDDAVSCEYRSRPVLLAKYPWGY
                     RGFFGIAPADPPGPGAITVLLATGSRLEKHHLPFTVSTTRFPVLRKTLNVGKYTNQSY
                     LAKPEVSAFIKKCADIKKEIFARATPNAIGARFSHPRDEHYVTSSFWMKRLYQRFTIK
                     KGRRVALAPQNNTHRGIDLRGKEGAPIFAIADGVVAYADTMFYEGNFTVIDHGNRIFS
                     YYMHQSKINVKTGDAVKAGDVIGLVGSTGISTGAHLHVSLVVNGVQVDPLSVLPIRAR
                     E"
     gene            complement(7532..7804)
                     /locus_tag="PKJ16_10220"
     CDS             complement(7532..7804)
                     /locus_tag="PKJ16_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004938186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HNV47406.1"
                     /translation="MAFALEKMLENEAHAVYRSAMEEKSKYTAGLLAMFLGIFGAHKF
                     YLGSHLQGALYLLFIWTAVPMVLSFIDAVQLLTMDDATFAKKYLGT"
     gene            7855..8577
                     /locus_tag="PKJ16_10225"
     CDS             7855..8577
                     /locus_tag="PKJ16_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV47407.1"
                     /translation="MHIIREPYARLEGIDTVAILADPGCRAGWERNFPPLLETVMREH
                     APQLFVVAGDMSLNSLDDEYRAIIRYMDRYEGRWASVPGDHDRPLRTFQRYFGATRKV
                     LDVGRWRFIGINTANRMFLRREREWIDAHIRPNSIIISHLPPEADGWTFHSLWPKSSD
                     HFLATVRRHQKKIHSMYFGHIHGYSARKYLGIPMIATGGVAESLIVRDNAYRGPGDFE
                     MVIFDVASGKTKLCVLKNPAKR"
     gene            8629..9189
                     /locus_tag="PKJ16_10230"
     CDS             8629..9189
                     /locus_tag="PKJ16_10230"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV47408.1"
                     /translation="MIVSVVLFSLAVIGTSADGSATTHCDTAAYVIDRDPDGLNVRTA
                     PAGAVITRIPAGAMVTITGQSGAWLRISSVTYPSDMHETFARTNKHQMNSGWVKLDKL
                     EGWVFAKNLGTTVRNYHPTKKAFLHAEPNIGSKKTIRFNDVEPQVTVLGCRNDWLYVE
                     HAGRMGWLSPSRNCPNSLTTCPGEYE"
     gene            9330..10679
                     /locus_tag="PKJ16_10235"
     CDS             9330..10679
                     /locus_tag="PKJ16_10235"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47409.1"
                     /translation="MTKQSRTAHENVRNTRSIHPTAQFNYLPRTIGCLLTFLILGSIF
                     YDNTNYAVWGALIFHGLIWPHCAYVHARFSTNSKTAEVVNLLIDSFIIGMWTNIIAYR
                     LWPLAALNIAILMDNMATGGLRLLLKGLFSTLVGVGLSVVLFGFSVSSEVSVLTGALS
                     AIAITFFTVVVSYTSYSLTLRYRNAKKALEERTQELQETRDALWGEMELAKKIQTVLL
                     PTEPDISGLDISCYMQPADEVGGDYYDVINAAGKNWLVIGDVSGHGVSAGLIMMMVQT
                     SINTAIEQNPEVEPSELLSIINRTIKRNIEKLDKDKYMTITLFAVFQEGRFFFSGLHQ
                     DILIYRSSTKTVDTVETDGVWIGLMNDISDFIKTDDLFLEHGDTMLLYTDGITEAWPR
                     NSETATRRQHMFGTNTLIELLQAYGDESTERIKKELLRAFEDYDTDDDVTFMVIKRV"
     gene            complement(11143..>11356)
                     /locus_tag="PKJ16_10240"
     CDS             complement(11143..>11356)
                     /locus_tag="PKJ16_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HNV47410.1"
                     /translation="KSKMLEYFRRIEQSGEELFMTDNRKPVLRVTPLNRKANPAVVFD
                     DFLGSVVHHEDIMADVLDELEPRTSR"
BASE COUNT         2427 a         3142 c         3327 g         2460 t
ORIGIN      
        1 cgtcggtcga gccctggcgg gggtgggcgc ctttgtgttg cgccatgtcg atcacttcgc
       61 cggccttgcg gattccgcga tgggcggcct cggcgatcgc ctcaggatgt ccgatggcgg
      121 tcacgaccgt atcgaactgg acgctttcgg tcgcgccgtt gtgctcgtag gtgaccttcc
      181 cgtccttcac cgagagcacc tttgcgttct tgatcacggt gatgccgtcg cgctcgacgt
      241 cggccatcac cacccagcgg gtggagcgcc cgatgtcctt gccgatcttc ggcagcatct
      301 cgaagatggt gacctccttg gagccccggg tcgcgagctc gcggatccgt tcgaccggct
      361 cggcgtcgcg caggaacagg aactcgagca cctccggcga gatcgtgccc ttcgccgcga
      421 tgaactgcgc cgtttcgatc cccacagcgc cgccgccgat cacggccacg cgccgcccga
      481 tcatcgggtc ctcgcgcagg accgtccacg cggacaccac gccgggtccg tcgatgccgt
      541 cgattttcgg cacgaagggc tccgcgccct cggcgacgat cacgtggtcg ggccgctccg
      601 ccgcgaccgt ctccgccgtc gcctcggtgt tcaggtggag cgcaatcggc agcgccctca
      661 gcatggcccg ataatacctg atgatctccc agagctcctg cttgtgcggc ggcgcgcccg
      721 cgatgtggag ctgcccgccg atgtcgcccg atttctcgta gagcgtgacc gcgtggccgc
      781 gctgcgacgc cgtgaccgcc gcctcgaggc cgcccggacc cgcgccaatc accatgattt
      841 ttttgggatg attcgtcggg atgatggcgc gctcaccctc gaagcccgcc atcgggttcg
      901 cgatgcacat caccggacgc gcgctgaaga gctcgtccat gcaaccctgc ccgcaggcca
      961 cgcaggggcg cacctcggcg gcgcggccct cgcggacctt gttgggccat tccggatcgg
     1021 cgatgaggac gcgcccgagg ttcaccatgt cggccacgcc gtcgcggatg atccgctccg
     1081 cggtgtccgg atcggggatg cggttggagg ccatcaccgg caccgacacc gcgcgcttga
     1141 tgttgagcgc gagataggcg aagccgccgc gcggcatctc ccgcgtgatc tgcggcacgc
     1201 gcgtctcgtg ccagccgccc gtcacgttga tgacgtcgat ccccgcgcgc tcgtaggcct
     1261 gggcaaagag cggcgtctcg cgatcggtgt tgcttccggg cacgaagtcg tttcccgcca
     1321 tgcggatgcc cagcggatag gacgcaccca ggcgcgcgcg catcgactgg atgatctcca
     1381 cggggaagcg cagccggttc tcgagactcc ccccgtattc gtcggtgcgc ttgttggtca
     1441 ccggggagag gaactgggtg atgagatagc ccgccgatcc gatgatctcc acgccgtcga
     1501 tccccgcctc cttcgcgcgc tcggcggcgc gaacgaaggc ctcctggagc tgtttgatct
     1561 cgtcgatgcc gagctcgcgc ggggtctcct ttttaaactt tgaatacacc gccgacggcg
     1621 cgaccggctt ctcgcccttg agaagaaatg aaaaggcgta cgcaccggca tgatagagct
     1681 gcacccaggg gctcgcgccg tgactgcgga tgtcgtcggc caggcgcttg aactgcggga
     1741 tggcgtcgtc attgtccagc gagatgggca ccccgcccga accgataaag tcgatcccca
     1801 ccgggccgat ggtgacgatg ccggccccgc cccgggcgcg ctccacgtag aagtcgtgat
     1861 agcgctgatt gagggtccca tcgtacgaat agagcagccc cagggaggga taggcgatgc
     1921 ggtttcgcac ctccagcgta ttgatgcgga tcggggtgaa caggctctcg tacatggctg
     1981 actccttttt gaaagataac agtgttatgt ttcgtggcaa ccatatcacc ctataataga
     2041 attgcaatat tttttttatc atacacatcg gccggtacga cctccccctt cgttcggggg
     2101 aggcgtgcaa atggtgatct tcctcggccc atgccatttc atggcgctgc gggcgtcggc
     2161 gcggatcatc acaaaaaatg ccgttatctg ttgacatcct gccgcgtggt cttcaaatgc
     2221 agaagaattc attccatccg cgaaggagcg catatggact acaaaaccat ccagttcgat
     2281 attatcgaga aggggattgg gcacctgcgg ctcaaccggc ccgaggcata caatgcggtc
     2341 aaccacctga tgatggagga gctcgacgca ttctggcggg agcgcctgta cgatctcgac
     2401 accgtcgtca tcatcctgtc gggcaacggc gagaagggct tttgcgcggg cctggacctc
     2461 aaggacacgg cggagatggc acccaagatg aacgtggccg agttctatcg attccaggcg
     2521 cgcctcgcgc ggatcaacct ggcgatgcgc cgggcgcccc agccgatcat ctgcaccgta
     2581 cacggcgcgg cggccggaca gggcttcagc ttttcgctgg cctccgatat tcgcatcatc
     2641 tcgcccgacg cgcgcttcaa cgccgcctac atcaacatcg gcctgggcgg ggcggacatg
     2701 tcgtgcagtt attttctgcc gcgcatgatc ggcgccggtc gcgcctatga attcatgtat
     2761 accggcatgt tcatgtcggc ccaggaggcg caggcgctgg gcatggtgag ccgcgtggtg
     2821 gagcgcgaca agctgcacga gacggcgctc gagatcgccc gtgtgatgat gaccaagaat
     2881 cacatgggtc ttcgcctgac gaaggaggcg gtcaacatga acctcgacgc gaacgggctg
     2941 gagcacgcgc tcaacatgga ggaccgcaac cagacgctgt tggccatcgg gacgttcatg
     3001 ggcaagaagt agcggtgcgc tcgtgatgaa tcatcaccct ccttggtgtt tcggggaggg
     3061 tggtatgcat attccctttc gatccctccc gcttccgata acagaacacg cgtttcgcgc
     3121 gtaaaacccg gcttcggcga ccaggaactc cttatccgat atgctttttc cttgagtttt
     3181 tcatcgacat catgtttaat gacggtagtg gaaatgccct ccagggggct ttccgaatat
     3241 tttggagggg gtattgatgt catggtaaaa aaagcgtttc tcggtattgg agtattgctt
     3301 tttatcgcgg gcggtttctt tatatttaaa accgtatcgt ttatgaacaa ttcaaacaag
     3361 gtcgatgcga aggttgtgtc ggtgcaaacc aaggtccaca aggggaagaa gatgtccagg
     3421 accacgacat ataaaccgac attcgaattt actgatgcag gcgggaaatc ggtcacggcg
     3481 acttcgtttg aaacatcaaa aagctacaat ttccccatag ggagttcggt tgagattttg
     3541 tatgataaaa acgactcgac caatataaag atccccactt ttttttcatt atggctgata
     3601 ccaacgattc ttataccgat agggttgatc ttctttattg ttggtttggt tggaaaaaga
     3661 aaataaatat ttacaacagg gcctccgaaa ggaggctctt tgttgtggtg ggctttgtgg
     3721 cataacggat ccggtataaa accatgaaac gaaagagttt actcgtatgc gtgctttttc
     3781 ttgtcgtgag ccaggggctg ttcggcggga acaccgccga cgagctgtac cgaaaggccg
     3841 ttgcgctgga aaagaagggg cgctacgggc aggcggcaca aacgatgaac gaggtgcttg
     3901 ccgcggtccc cgggaatgat ttttacctcg cctatgcgag ccatgtggag cgcctcgccg
     3961 gcgattacga aaacggcctc cggcacgcca tcaccgcgat ttcgctcaac ggcgccgtgg
     4021 gctggtacca tgcctcggcg atgtgtaacg cctggggatg cgcggacatc gatgcgacgc
     4081 gcacgtatgc ccggaaggtt gtctccctcg ggccgacggc ggcggggaag gaaaacttcg
     4141 attatgctca aacgctgtta aagagccttt ccaggagggt ctatacgatc acctggcgcc
     4201 ttgatccgtc gaagggagtg aaaaccgatg gccggtatcg cgtgccgctt cccaccgacg
     4261 gtttgccgta ccagcgcgca cggttcgatg tcgagggctg ctccgagcga acagtcgtcg
     4321 tggctgaagg taacgatgtt ctgctgtttt cttccgccgg cggcggagtc gtcacgatcc
     4381 gggccgagat tgcaatcact cccttcagtt ttcggaagcg tctttcgggg catgcggcgc
     4441 gcggcgattg ccgggttccg gacgatgccg cgccgtatct cgggcgaagc gatcgcgtcg
     4501 atccgaacag cgccgcggtt cgaatggtcg ccggccccct gaagcatcgc gagccgatgg
     4561 tttcagtccg gaatatccta tcctggatga ataaaaacat caaatacgaa ataaaggatt
     4621 tccggcatgt cgatgaaatc ctccgccggc gaaacgccga gtgcggcggg tggtcggccc
     4681 tcttcacggc cctgtgccgc gccaatggca taccggcgcg tgaggtctgg ggggtcatcg
     4741 aggaccccac gcctgatcgg cgtttcgcgc ccgccggaca tctcaagggc catgcctggg
     4801 ccgagttcta cctgtgcggc atcggctggg tgccggtgga gccgcaacgg ccggacacgc
     4861 tcggtttttt gcccgcgacc tatgtgcgca tgtatcacta cgacgtcgta agttcgttga
     4921 cctcgaccca tgcctatcgg ccaaacggca acatggtgct tatgaacggc gacactcccg
     4981 cattcgtggt ggacgtcaag gagtgaggcg ggcatcgtca tgacgttgat tgtcgtcacg
     5041 acgctggcat gcccgaatgt cgtgtttgga cctattgttg ataacgagaa aacggtcgtt
     5101 cgatggcagg cgccgattta acatgcaccc tcgggaacgg gtcacggcga ctggatcgag
     5161 atcatatggc aggagggagc gaactgtacc atcgcgtcgg gcaaacggtt atttttctaa
     5221 cagatggcgc agatacctcg acagctcgtg cccgttcgcg ctgtcggatt cgatgatgct
     5281 gttaatcgca gcatgtgccg atacaccgcg cggatttccg gatgatcggg ttttcccgac
     5341 ccacgtcggg tgcttacggc cggatcgcct tcccgaccga aggcggattg cccgggtgac
     5401 cggtgctcgc cggaacctgg ttaaacaaaa ttcaaccgta aagcggcatg atcccatata
     5461 tgtggcaata tcgcaaccac ggacattata atgcccctgc cccacaatat acctcttgtc
     5521 gatacatcgc gggagaaagg cgcgctggtt gaggtttatg cggcgtgcag cggcaaatca
     5581 tgcaataccc gcattcgttc ggacggaaaa tttgctgttc gactggataa aaacaatacg
     5641 agacccgaaa tctatgatgc gtttggaaaa gcggcccaaa aagcgtccgg cgattcggca
     5701 ttaatgcgcg ggaagagccc ggtattcata gaactaatac acaacctgca tacagcacaa
     5761 gcacagagcc tcagaaatgg aattatcgtt tacctcggtc ttcttgcgct gtggtttgcg
     5821 gtgtcgatct ggtcaatacg agcggacgca aagtcctgac gaaatggcgt tgtgtgcggg
     5881 cagaatgcgg agggagggat gagaatgcaa ggcctggagc acctgattcg acagacgtac
     5941 ccgaatgtaa atatcgcgaa gacgaagcgg ctcgggaaaa tgagaatacg catgagcagg
     6001 cccggggcca tgtcgtggca aaaattcaag aacgtggaca tggtggaaac ggaccatgca
     6061 tattttttcg atcctcggga gggcctgatc aagagctaca aaaggcaact gctgatctgg
     6121 tcggtgttgc taccgctctc ggtgatcctc gtcgtcaacg gcctgttccc agcggtgatc
     6181 gtcatggtca tcagcgccct cggggtcatc gcctgtccga tcaatctggc gatgtcccgc
     6241 aagccgaaga ggatcatcat gatatggaaa aatcgaatcc ggacaaacga tgtcgtggac
     6301 ggggagcaca ttctccaggg tgccctcatg aaaggtgacg gcttttccat cgttgcggac
     6361 atgaaggagg tgagtgatgg aggaaccatc gggtatgacg ccgactttat cattcgctgt
     6421 accgtttgat aatcgatcgg tcactcccgc gccctgatcg gaagcacgct cagcggatcg
     6481 acctgcacgc cgtttaccac cagcgacacg tgtaggtgtg cgccggtgga tatgccggtg
     6541 cttcccacca ggccgatgac gtcccccgcc ttcacggcat cccccgtctt cacgttgatt
     6601 ttgctctgat gcatatagta cgagaagata cggttgccgt gatcgatcac cgtgaagttt
     6661 ccctcgtaga acatcgtgtc cgcgtatgcg accacgccgt ccgcgatcgc gaagatgggt
     6721 gcgccttcct ttccgcgcag atcgatcccg cgatgcgtgt tgttttgggg cgcgagcgcg
     6781 acgcgccgtc ccttcttgat cgtgaagcgc tgatagagcc gcttcatcca gaaggatgag
     6841 gtgacataat gctcatcgcg cggatgcgag aagcgcgcgc cgatcgcatt gggcgtcgcc
     6901 cgcgcgaaga tctctttctt gatatctgcg catttcttga tgaacgcgct cacctcgggc
     6961 ttcgccagat agctctggtt ggtgtacttt cccacgttga gcgttttccg aagcaccgga
     7021 aagcgcgttg tcgagaccgt aaacggcagg tggtgttttt caagccgact ccccgtggcc
     7081 aggagcaccg tgatcgcacc cggcccgggc gggtcggccg gtgcgatgcc gaagaagccg
     7141 cggtaccccc acgggtattt cgcgagcaac acgggccgcg atcggtactc gcacgagacc
     7201 gcatcgtcga tggcgcgttc gcgcggcgtg atttccacgt acacgatgtc gccctgcgag
     7261 aagccgcgcg aggtgagctt cacggttacc cggtcgcggt cccatttcag gtccttcgcc
     7321 gcggggacct ggtagccggg cggcaccttc gccggagggg acgcggtgcg tacgcctggc
     7381 gcggccgccg tattcgagac ggtcagaatg gcaagcagca ccgtgcgcgc tatatgatta
     7441 tgtctcattg aaatacgcca tcacccctgt cgatatgagc gttcattgac gaccggcctc
     7501 ttttgcgtga accgaacgga acgcggcggg actacgtccc gagatacttc ttcgcgaagg
     7561 tggcgtcgtc catcgtgagc agctgcacgg cgtcgatgaa cgagagcacc attggcacgg
     7621 ccgtccatat gaaaagcagg tagagcgcgc cctgcagatg gctgcccaag tagaacttgt
     7681 gcgcaccgaa tatcccgagg aacatcgcga gcaggccggc tgtgtacttg cttttttctt
     7741 ccatggccga acggtaaacg gcgtgcgcct cgttttcaag cattttttcc aatgcgaacg
     7801 ccatcggcga attcggcttg acgcatcaaa attgtggggc gcaggatagg cgtgatgcat
     7861 atcattcgcg aaccatatgc gcgcctggag gggatcgata ccgtcgccat cctggccgat
     7921 ccggggtgcc gcgccggctg ggagcggaac tttccgcctc tgctggaaac ggtgatgcgc
     7981 gagcacgcgc cgcagctctt cgtggtcgcc ggcgacatgt cgctcaattc gctcgacgac
     8041 gagtaccgcg ccatcatccg atatatggac cgctacgaag gacgctgggc gagcgttccg
     8101 ggcgaccacg atcgtccgct gcggacgttc cagcgatatt tcggcgcgac gcgcaaggtc
     8161 ctcgacgtgg ggcggtggcg ctttatcggc atcaacaccg cgaaccgcat gttcctgcgc
     8221 cgcgagcgcg agtggatcga cgcgcatatc aggcccaact cgatcatcat ctcgcacctc
     8281 cctcccgagg cggacggctg gaccttccat tcgctctggc cgaaatcctc cgaccatttt
     8341 ctcgcgaccg tgaggcgcca ccaaaagaag atccattcga tgtacttcgg ccacatccac
     8401 gggtattccg cgcgcaagta tctcggcatc ccgatgatcg cgaccggcgg ggtggccgaa
     8461 tcgctcatcg tgcgtgacaa cgcctatcgc ggtcccggcg atttcgagat ggtcatcttc
     8521 gacgtcgcga gcggaaaaac gaaactgtgc gtgctgaaga atccggcgaa gcggtaattc
     8581 ttgcacggaa ggattattgt gaaattaatt ttcccatata tttgcgtgat gatagtatcg
     8641 gtcgtactct tctcgctggc cgtcatcgga acttctgcgg acggatcggc aaccacccac
     8701 tgcgacactg ccgcgtacgt gattgacagg gacccagacg gcctcaacgt gcgcactgcg
     8761 ccggccggcg cggtgatcac ccgcatcccc gctggggcca tggtcaccat caccgggcag
     8821 agcggcgcgt ggctgcggat ctcctcggtc acctatccgt ccgacatgca tgaaacattc
     8881 gcccgtacaa acaagcatca gatgaatagc ggctgggtaa agctggataa gcttgaggga
     8941 tgggtgttcg cgaaaaatct ggggaccacc gtgcgcaact atcatccgac aaaaaaggca
     9001 ttccttcacg ccgagccgaa catcgggtcg aaaaaaacca ttcgattcaa tgacgttgag
     9061 ccgcaggtga cggtacttgg gtgccgaaat gactggctgt atgtggaaca cgcggggcgc
     9121 atgggatggc tctccccgtc gcgcaattgc cccaattcgc tcacgacatg tccgggtgag
     9181 tatgagtgaa tgacgagatg aagaactttt gattattgta ttgtgattac attgcgagac
     9241 tatatgtaaa ttccaatatg catcaggggc gcgtgtctca cacggatcat atgggacatg
     9301 cgtagtaaca atactgaagg tgtgttcaca tgacaaaaca atctcgaacg gctcatgaaa
     9361 atgttaggaa cacccgctca atccacccta ccgctcaatt caattacctt ccgagaacca
     9421 tagggtgcct tctgaccttt ttaattcttg ggtcgatatt ttatgataat acaaattatg
     9481 cggtatgggg agcactgata tttcacggat tgatctggcc gcattgcgcg tacgttcatg
     9541 cccggttcag cacaaattcg aaaacagcgg aagttgttaa cctgctgata gattcgttta
     9601 tcatcgggat gtggacgaat ataatcgcgt atcgactgtg gcccctggcg gcgctgaata
     9661 tcgcgattct catggacaat atggccacgg ggggactgag gctgttgctg aaaggcttgt
     9721 tttcgactct cgtgggtgta ggactgtcgg ttgtcctgtt cggatttagc gtatcatcgg
     9781 aagtaagtgt attaaccggc gcattgtcgg caatcgccat cacctttttt acggtcgttg
     9841 tcagttatac ctcgtacagc cttaccctca ggtaccgaaa cgccaaaaaa gctctggagg
     9901 agcgcactca ggaactccag gagacccgtg acgccctctg gggagagatg gagcttgcaa
     9961 aaaaaattca aaccgtcctt ttgccgactg aaccggatat ttccggtctt gatatttcat
    10021 gttacatgca gccggcggac gaggtgggcg gggattatta tgacgttatt aatgccgcgg
    10081 gcaaaaactg gctggtgatt ggcgatgtct cgggacatgg cgtttcggcc ggtctcatca
    10141 tgatgatggt gcagacgtcc atcaatacgg caattgagca gaaccccgaa gtggagccgt
    10201 ctgaattgct gtcgataata aacagaacga taaaaaggaa tattgaaaaa ctcgataaag
    10261 acaagtatat gacgataacg ctgtttgcgg ttttccagga aggcagattc ttcttttcgg
    10321 gtcttcatca ggatatttta atatacagaa gcagcaccaa aaccgttgat acggttgaaa
    10381 cagacggcgt gtggatcggt ttgatgaatg acatcagtga ttttattaaa accgatgatt
    10441 tgtttcttga gcatggcgat acgatgcttc tctatacgga cggcatcact gaggcatggc
    10501 ctagaaactc ggaaacggcg acaagaagac aacatatgtt cggaacgaat acgctgattg
    10561 aattattgca ggcatatggg gatgaatcaa cagagagaat caagaaggaa ctgctcaggg
    10621 catttgagga ttatgacacg gacgacgatg tgaccttcat ggttataaag cgggtatgac
    10681 agaataagca aaaagttagt ttgtgaaaat agttatattt cgcagggcgg cgattacgat
    10741 gggttgccgt taattcgcaa ccgcctttga agcgtcgcta caccatgatg aacgtaaaca
    10801 ataacatgcc cgaccatggc gcttttttcc catggaggca cgcatggaat gaaaaaaaat
    10861 tgacttcagc taaaatcaca gatagttttc aggtaagtca tcatgcacaa atcaatggag
    10921 gaatgtgatg aaaaaggtat tggttttgct tgttatattg atggtcacct cgacaatatc
    10981 gacatgctgg tgcaagcacg atcatggcag gcataagggg tggtttaagt acgatcgtat
    11041 gccagaccaa ccggagcgct gaaaactata tgccgtgatg ccagcttcga catgaaccct
    11101 ccaatacggt gggttcatgt cgtctggagc gctgttgaaa cattaccgcg aagttcgagg
    11161 ctccaattcg tccaagacgt cggccatgat atcttcatgg tgcaccacag agccgagaaa
    11221 atcatcgaaa acgacagctg gattcgcctt gcgattcagc ggcgtgacgc gcagcaccgg
    11281 cttgcgattg tccgtcataa acagctcctc gccggactgt tcaatcctcc tgaagtattc
    11341 cagcattttc gatttc
//
LOCUS       DAORHF010000108        11311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4921_length_11311_cov_5.540956, whole genome shotgun sequence.
ACCESSION   DAORHF010000108 DAORHF010000000
VERSION     DAORHF010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4921_length_11311_cov_5.540956"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3479)
                     /locus_tag="PKJ16_10245"
     CDS             complement(<1..3479)
                     /locus_tag="PKJ16_10245"
                     /inference="COORDINATES: protein motif:HMM:NF021619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47411.1"
                     /translation="MGAKILIVDDELIFARDVERRLTEFGYEVVGTASTGPEALALAK
                     QCAPDLALMDIVLEGAIDGVTAASEMHATYGVPVVFVTGNADQATVERAREAKPYGYV
                     LKPVSPLDLYSTIDTVLRRASLEREVVRQKEELAALNEELTQALEEMEQSNEELATSN
                     EALAGTQAELEERNRQLAQNGELLRLRLDAILEPDVEIDEREFAAIVDTGAVQSLMDD
                     FYALTGIGVAIIDLNGAVLVATGWQDICTQFHRVNPETARNCVESDVFLSQGVPPGAY
                     RKYKCKNNLWDMSTPIMAGGKRIGNLFLGQFFIEGEVPDRELFREQARRYGFNEDEYL
                     TALDRVPRWSERTVETVMRFYAKFANLLSRLSYETVSLARTNQSLKEAHAENESIQGL
                     LAQREEHYRRLYEQSPLGYQSLDADGRFIDVNTAWLELLGYEREDVIGRWFGDFIIPE
                     QVEAFKKRFPQFKAAGSVSTEFVMLRKDGSLVTISFDGRIEYTEKGDFRQTHCILKDV
                     TEQRRLEEAVEYERNKFNTIFSAAPVGILLLDAETEVVNANDAAGAMVLREPAELIGE
                     RMGGALQCVHSAEEPRGCGFSVACPVCPLRRAVEEVLAGGERVRGATVEVELMVRERR
                     ERRWLRINAEPIALRDSRNVIVTVDDMTDVVFAERKRAAAEERAIEERNRLFAVLDLL
                     PAYVYLIDERFRIVYANRLFKEYFGEPGGRTCYDLFHGRTEPCEKCPAMRVFTDGRMQ
                     VREWTHPSGRVFEAYNYIFPVPGDAPMVLEVGLEVTEWKRAQEGLRASEEKYRELVEN
                     ANSIILKMDPSGNIAFINEFAQRFFGYAPEEVLGKSVVGTIVPRVESSGRDLAELIAG
                     ICVNPERYAYNENENMKSSGDRVWVSWTNKPVFDAGSRLVEILCIGNDITERKRMEDE
                     LVKSREEYRSYFNMSTIGMCVTSPEKGWVEVNDYLCDMLGYSKEELQTRTWAELTHPD
                     DLDADVTQFNRLLSGEVEDYALNKRFIRKDGGIVYTRLYVTCLRNADGSARSVYASLV
                     DITEQKAFEGRLSANERYYRTLLDNSFDAIALMNDRGEFMYVSPSVERILGYAPNELI
                     GRLSQGFTTPEMAASLNEQLGLVLANPGGVRSAEWTAFHRDGREIILEGVAQNLLHDN
                     ILRAIVINFRDIT"
     gene            complement(3888..4526)
                     /locus_tag="PKJ16_10250"
     CDS             complement(3888..4526)
                     /locus_tag="PKJ16_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV47412.1"
                     /translation="MLIVDDHPIVRQGLLQIINREPDLVVCGEARGADEALRFVSNTL
                     PDIAVVDISLEGSSGIDLTRALRERYPDLPVLVLSIHDEAIYAERALRAGARGYVMKQ
                     EAPGRVVGAIRTVLEGRQYLSEAMKETIMRKVSPDAAAPGASPEETLSDRELEVFQLI
                     GQGLANRAIADRLRVSIKTVENLRERIKTKLGIATSSELVPFAIQWTLAKNK"
     gene            complement(4623..6035)
                     /locus_tag="PKJ16_10255"
                     /pseudo
     CDS             complement(4623..6035)
                     /locus_tag="PKJ16_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(6032..8917)
                     /locus_tag="PKJ16_10260"
     CDS             complement(6032..8917)
                     /locus_tag="PKJ16_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47413.1"
                     /translation="MLKRAPNFSSVRVAALFWVCGLALSLLAVFGLSMYLVRMSDIRA
                     AETERLEAQRDARARAIGQWLDRIAGDLRTLAAMPEVRSNAALMGSGALSGHQASAPF
                     RGVFNAYLHNYNLYYEFFIISARLGKIVYSTTRENIGEARPAESYFSDAIRDNDILVK
                     DINFSKNLSIPTMTVAVPLHASPQGDAPLTGYLVARIYLDRSLYELLGERGGLGSSGE
                     ALLVNGRHKLMNRVRGLEGAPLSQRLDDPASRLAAEGKTGAMEVTDFRGRTALAAYTH
                     LPRLRWGLVVKKDAEEVYAPARRMLGEMALAFVVALALLAYVGVRLGSGLAAPLEALS
                     SVMRRIGGGEYAVRASIPGRDELGRLAESFNAMADSITAELQLQKESSDIIEIMVSAV
                     ELHDFSTGVLRALVEMTASSIGAFYVLSPDGREFVHLTSIGLDANAMETFHAEKLEGE
                     FGRALATRRITRTRNISGTSMLRLRTVIGDVVPTELVTVPVVVNNRCVAIISLASLGE
                     YSEMAIKVLNLVWPVMNTSFSNIIANEETRVLARELRSKNQLLEMQKEELQAKAEELR
                     RQTERVNQQNAELEEQRARVEEANRLKSEFLSNMSHELRTPLNAILSLSKVLIRQAAE
                     RLPADEVGYLEIIERNGRTLLALINDILDLSKIEAGRIDLKPQRLSLPAVVGAIIENL
                     ERSAQEKGIALEARFAAGFPEIESDEQRVYQIVQNIIGNAVKFTERGGVTVAGRFDAH
                     TVTIEVADTGIGIAENDIPTIFEEFRQIDGTLSRKYGGTGLGLAIASKSARLIGGAID
                     VASTPGTGSTFTVRMPIAWGGACVPAPQRARPADNRANDGSGRPLILVIEDDPDNLTT
                     IKALLAGHGTVIEATTGRAGLERVRAERPDLVLLDMALPEMNGFEVARAIKDDPDTAA
                     LPVIAVTALTMDGDRERALAAGCDDYLPKPIDIDALFVTIRRWVRLA"
     gene            complement(8924..9418)
                     /locus_tag="PKJ16_10265"
     CDS             complement(8924..9418)
                     /locus_tag="PKJ16_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47414.1"
                     /translation="MGALIADLFKEHRGIAEFRESIVGLGGRFAFEPGDMLELGAVYF
                     ERYPDCFSNRNCQEVQLGYQMVRICVVEKAIADLPEPSRQAYRTMFFSIRDIDARVDS
                     IVAARGADAALEELAQVERGVASLKDAIDTLPTGMIKERFVGGISNIFNVVYLIKTSL
                     ARHR"
     gene            9441..9617
                     /locus_tag="PKJ16_10270"
     CDS             9441..9617
                     /locus_tag="PKJ16_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47415.1"
                     /translation="MRIVAEHPFFFNQETDGISARPGYGIAFLLRKSARMIGSVHRDY
                     RTDTPHPQEEPDPS"
     gene            9614..10915
                     /locus_tag="PKJ16_10275"
     CDS             9614..10915
                     /locus_tag="PKJ16_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HNV47416.1"
                     /translation="MTADRPASLVFAQSGDACFRYRPTERESEVLERINRKIAALDTL
                     EQTIDFLFAETQGIMPCDRLAVAFFEDEGTRLTIHHVVSRTEPRRLTKGYSADIARSS
                     LRRVFETGCPRIINDMDEYATAHPDSESARLILMEGIRANMTCPLTVDGRPVGVLFRS
                     ASAPGVYADREVALHLMIAERLSQAVEKAWRIEQLSSTVNAYLETLAFVTHELRSPLD
                     SIATMGKTIVNGYLGPVDARVAEFVGRMVKKAEYLSGISNDYLNLARFESGGASLRTA
                     TVDFITDIVDEAIDIIRPQMEEQSVRLETDYRLGGRPVSCDPALMKTAMINLLGNAVK
                     YGNREGVVRITATDDTGALVVSVWNEGPGFSVEDRARLFRRFSRLPKKELMARKGSGI
                     GLYVTWKILQLHGGRIQAQSEEGRWAEFTFEIPLGNDTEST"
     gene            11053..>11311
                     /locus_tag="PKJ16_10280"
     CDS             11053..>11311
                     /locus_tag="PKJ16_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47417.1"
                     /translation="MKKFSLVLFILVIAVSFAAISCSGQKKSGAASSGNMVVDALNAS
                     LAKVPIEGFQSGKAASKQEWDAMITKAIPVVKEVLPKVPDGY"
BASE COUNT         1952 a         3793 c         3504 g         2062 t
ORIGIN      
        1 tcggtgatgt cgcgaaagtt tatcacgatc gcacggagga tgttatcgtg aagcaggttt
       61 tgggcaacgc cctccaggat gatctcgcga ccatcgcgat ggaacgccgt ccattccgcg
      121 cttcgaacgc cgccgggatt tgcgagcacc agcccgagct gttcattcag ggaagccgcc
      181 atttccgggg tcgtaaaacc ctgcgacagg cgtccgataa gttcgttcgg tgcatatccg
      241 agtatgcgct ccaccgaggg agatacgtac atgaattcgc cccggtcatt catgagcgct
      301 atcgcatcga acgaattgtc gaggagcgtg cgatagtagc gctcgttcgc ggaaagccgg
      361 ccctcgaagg ccttttgctc ggtgatgtcg accagcgagg cgtacacgct ccgcgcggaa
      421 ccgtcggcgt tccgcagaca ggtcacatac agcctcgtgt aaacgatccc tccatccttc
      481 cggataaagc gcttattgag cgcgtaatcc tcgacctctc ccgagagaag ccggttgaac
      541 tgcgtcacgt cggcgtccaa atcatcagga tgcgtaagct cagcccaggt gcgcgtctgc
      601 agttcctcct tcgaatatcc cagcatatcg cagaggtaat cgttcacctc cacccagccc
      661 ttctcggggg aggtgacgca catgccgatg gtgctcatat tgaagtagct gcgatattcc
      721 tcccggctct ttacaagttc gtcctccatg cgctttcgct cggtaatgtc gttcccgatg
      781 cacagaatct ccaccagccg gctgccggcg tcgaagacgg gcttgttggt ccagctcacc
      841 catacgcgat cgccggagct cttcatgttc tcgttctcgt tgtacgcata ccgttcggga
      901 ttcacgcaga tgcccgcgat aagctccgcc aggtctcgcc ccgagctttc gactcgcggg
      961 acgatggtgc ccaccacgct cttgccgagc acctcctccg gcgcatagcc gaaaaagcgc
     1021 tgcgcgaatt cgttgataaa ggcgatgttc ccggacgggt ccatcttaag aatgatgctg
     1081 ttcgcgttct ccaccagctc gcggtatttt tcctcgctgg ctcgcagtcc ctcctgggcg
     1141 cgcttccatt cggtgacctc caggccaacc tcgagcacca tgggcgcgtc gccgggaacg
     1201 gggaagatgt aattataggc ctcgaacacc cgaccggacg ggtgcgtcca ttcccgcacc
     1261 tgcatgcggc cgtcagtgaa cacgcgcatt gccgggcatt tctcgcaggg ctcggttcgc
     1321 ccgtgaaaca gatcgtaaca ggttcggccg cccggctcgc caaagtactc cttgaacaac
     1381 cggttggcat agacgatcct gaagcgctcg tcgatgaggt atacgtacgc gggcagcaga
     1441 tcgaggacgg cgaagaggcg gttgcgctcc tcgatcgccc gctcctccgc tgcggcgcgc
     1501 ttccgctccg cgaaaacgac gtcggtcatg tcgtccaccg tcacgatcac gttgcgcgaa
     1561 tcccgtagtg cgatcggctc ggcgtttatg cgcagccagc gccgttcgcg ccgctcccgc
     1621 accatgagct ccacctcgac cgtggccccc cgaacgcgct cgcccccggc gagcacctcc
     1681 tccaccgcgc gccggagcgg gcacacgggg caggcgacgg aaaacccgca gccgcggggc
     1741 tcctcggcgc tgtgtacgca ctgcagcgcc ccgcccatcc gctccccgat gagttcggcg
     1801 ggctcgcgca ggaccatcgc ccccgcggcg tcgttggcgt tcaccacctc ggtctcggca
     1861 tcgagcagca ggattcccac gggcgcggcg gagaagatgg tgttgaattt gtttcgttca
     1921 tattcgacag cctcttcaag acgacgctgc tccgttacgt ccttcaggat gcaatgtgtt
     1981 tgccgaaagt cgcccttctc tgtatactcg atcctcccgt caaaggagat ggtaaccaag
     2041 ctaccgtctt ttcggagcat cacgaattcg gtgcttaccg aaccggccgc cttgaattgg
     2101 ggaaaacgtt tcttaaatgc ctcgacctgc tccgggatga taaaatcgcc aaaccatctg
     2161 ccaatcacgt cttcccgctc atacccaagc agttccagcc acgcagtgtt cacatcaata
     2221 aaacgaccgt cggcatccaa cgattgatat ccaagcgggg actgctcgta tagccgccga
     2281 taatgttcct ctcgctgcgc aagaagccct tgaattgatt cattctccgc gtgcgcctct
     2341 ttaagtgatt ggtttgtccg cgccagcgaa accgtttcgt agctcaggcg cgagagaagg
     2401 ttcgcgaact tcgcgtagaa gcgcatcacg gtttccacgg tgcgctcgct ccagcgcggg
     2461 acgcggtcga gggctgtgag atattcatct tcattgaagc cgtaccgccg cgcctgctcg
     2521 cggaacaact cccggtcggg gacctcaccc tcgataaaga actgtcccag gaacagattg
     2581 ccaatgcgct tcccgccggc catgatgggc gtggacatgt cccagagatt gtttttgcat
     2641 ttatacttcc ggtaggcgcc ggggggaacc ccctgcgaca ggaacacgtc gctctcgacg
     2701 cagttgcgcg ccgtctcggg attcacccga tggaactgcg tgcagatgtc ctgccagccg
     2761 gtcgcgacca ggaccgctcc attgaggtcg atgatggcga cgccgatccc ggtgagcgcg
     2821 tagaagtcgt ccatgagcga ctgcacggcg ccggtgtcca cgatcgcggc gaactcgcgc
     2881 tcgtcgattt cgacgtcagg ctcgaggatc gcatccaggc gcagccgcag gagttccccg
     2941 ttttgggcaa gctgccggtt gcgctcctcg agctcggcct gcgtgccggc gagcgcctcg
     3001 ttcgaggttg ccagctcctc gttcgactgc tccatctcct cgagcgcctg ggtgagctcc
     3061 tcgttgagcg cggcaagctc ctctttttgg cgcaccacct ccctctcgag cgatgcgcgg
     3121 cgcaggacgg tgtcgatggt ggagtagaga tcgagggggc ttacgggctt gaggacatag
     3181 ccatagggct tcgcctcgcg cgcgcgctcg acggtggcct gatcggcgtt gcccgtcacg
     3241 aacacgacgg gcacgccgta tgtcgcgtgc atctcgctgg cggccgtcac gccgtcgatc
     3301 gccccctcga ggacgatgtc catgagcgcg aggtccgggg cgcattgctt tgcgagcgcg
     3361 agggcttcgg gcccggtgga ggccgtcccg accacctcgt agccaaattc cgtcaggcgg
     3421 cgttcgacgt cgcgcgcaaa gatgagttcg tcatcgacga tgagtatttt cgctcccaaa
     3481 agaccccctg gcaatggcct ctgtgtatat ggttgaatat atgcggtcgc ggcgaaatgt
     3541 caatccaagt tgacccgccc gagcttcaca tcgccccaat tttttatttc agaaatcacc
     3601 tagaatcggc ataggaaacg acctactcca tccgcttcga taagctcagc gcaacgcccc
     3661 agccccgtcg aacgcctttc gggcatcctg cccgcgctcg ccactgcgac atcctgtcgg
     3721 gagccttctc ccacagggag aagggggcgt gaccgacaat tagcttgtcc cgaaggagcc
     3781 ctctcctacg ggaggcagca tcttgcacat ccgtgtgcca tgctgcatac gctacatcct
     3841 gtcgctaagg gcggcccaga gggaaggcgg ggcgaggtca cgattctcta tttatttttc
     3901 gcgagcgtcc actggatcgc gaacggcacc agctccgacg aggtggcgat gcccagcttg
     3961 gtcttgatgc gctcgcgcag gttctccacg gtcttgatgc tcacgcgcag ccgatcggcg
     4021 atcgcgcggt tggccagccc ctgcccgatg agctgaaaca cctcgagctc ccggtccgag
     4081 agcgtctcct ccggggaggc gccgggcgcg gccgcgtcgg gcgagacctt gcgcataatg
     4141 gtctccttca tggcctcgct cagatactgc cgcccctcca gcaccgtccg tatggcgccc
     4201 accacgcgtc ccggcgcctc ctgcttcatc acgtagccgc gcgccccggc gcgcagcgcg
     4261 cgctcggcgt aaatcgcctc gtcgtggatc gacagcacca ggaccggcag atccgggtag
     4321 cgctcgcgca gggcgcgcgt gaggtcgatc ccgctggagc cctcgagaga gatgtccacc
     4381 acggcgatat cgggcaacgt gttcgagacg aaccgcagcg cctcgtccgc gccgcgcgcc
     4441 tcgccgcaga cgaccaggtc cggctcgcga ttgatgattt gcagcagccc ctggcgcacg
     4501 atgggatggt cgtccacgat gagcacccag tgcttctcgc ggtcggcgcg gaagcgcccg
     4561 cggtagtact ccgcgaaatc ctcgcgccgc gccgcccccg cgccaagaaa gctcacggcc
     4621 acgtcggtcc cgcccgacgt gttccgctgc acggacagcg atccgccgat cacccacgcg
     4681 cggtaatgca tgatggagag gcccatcccg cggccggttg cctcgtccgg gatgccggaa
     4741 ccgtcgtccg aaacccggac ggtgacggaa tcgttttccc gacgtgcggt gatgacgatg
     4801 ttcttcgccg cgccgtgccg gaaggcgttg ttcacggcct ccttgacgat atagaacagg
     4861 tgcgtcgcct cgaggtcgcc cgccacgtcg attccctcgt cccacgtgag cagacaggcg
     4921 accgggaaga gccgctcggt gttcgcgcac agatccgcga gcgccgcgcg cagtcccccg
     4981 ccgtccatgt ccaccgggca caggccccgc gcgagacccc gcgcctggtc gatcgcctgc
     5041 tgcacgagct cgccgatctc ggcgatgtct cccgcggcgt agggcgcgcg ctcggcgagc
     5101 ttgagcttga cgatctcgct cttgaaggca atgcccgcga ggtgctgtcc caggccgtcg
     5161 tgcaggtcct gcccgatccg ggcgcgctcc ttctcgatct cgcccagcac ccgcttctcg
     5221 agcttcgtct tctcggtgat gtcgatccct acgccgaaga agtaatccac cgtgccgtcc
     5281 gcggacagga tcgcccggcc gtgccactcc accagccgct cctcgccgct gcgcgcgatg
     5341 atgtggttct cgttcgtggt gccgggcccg ccccgcgcga ggtcctcgaa gacgcccgcc
     5401 agcatggcgt gctcgcgcgc cgggacgaat gtttcaagat atggtttccc cgccgcctca
     5461 tcgagagcgc accccagcgc atcgagcagt gcgcggttca tggtgagcac cgtgccgtcg
     5521 acgccgatgg ccacgaagaa ggccggggac gattcctcga gcgtccgcag gaaatcgcgc
     5581 tcgcggcgga gctcgctggt cttctgctgc acggtcgatt cgagccggtc gcgctcccgc
     5641 cgcaggcggt cctcggcgcg cttgatgcgc agcatcaccc tgatctgcgc cacgagctcg
     5701 gcctcgtcga tgggcttggt gagaaatgcg tcggcgccga tttcgagccc gtggacgcgg
     5761 ctctccacgt cggtgcggat ggccgtgagc aggatcaccg gcacgtgcgc cgtttcgggg
     5821 agcgccttga gacgggcgca ggtctcgaac ccgtccaggc cgggcatctt cacgtccagg
     5881 atgatcacgt cgggcgactc ccgcacggcc agggcgatcc cctcctcgcc cgagagcgcc
     5941 gtgatcacgc gctcctccgg catgaggttc cgcaggaccg cggtgatggt gacgaggttg
     6001 tcctgcttgt cgtcgacggc gaggatggtg ctcatgccag acgcacccac cggcgaatcg
     6061 ttacgaagag cgcgtcaatg tcgatgggct tgggcaggta gtcgtcgcag ccggccgcaa
     6121 gcgcgcgctc gcggtcgccg tccatggtga gcgccgtgac ggcgatcacg gggagtgcgg
     6181 ccgtgtccgg atcgtccttg atcgcgcgcg cgacctcgaa gccgttcatc tccgggagcg
     6241 ccatgtccag gagcaccagg tcggggcgtt cggcgcggac gcgctcgaga cccgcgcgcc
     6301 cggtggtcgc ctcgatcacc gttccatgcc cggccaggag cgccttgatg gtggtcaggt
     6361 tgtccgggtc gtcctcgatc accagtatga gcggccggcc cgaaccgtcg ttcgcgcgat
     6421 tgtccgccgg tctagcgcgc tgcggcgcag ggacgcaggc gcccccccag gcgatcggca
     6481 tgcgcaccgt aaaggtcgat cccgttcccg gcgtgcttgc gacgtcgatc gcgccgccga
     6541 tgagacgtgc ggatttcgac gcgatggcga gccccagccc cgtgccgccg tatttccgcg
     6601 acagcgtgcc gtcgatctgg cggaactcct cgaaaatcgt cgggatgtca ttctcggcga
     6661 tgccgatgcc ggtgtccgcg acctcgatcg tgacggtgtg cgcgtcgaac cgaccggcga
     6721 cggtcacgcc gccgcgctcg gtgaacttca ccgcattgcc gatgatgttc tgcacgatct
     6781 gatacacccg ctgctcgtcc gactcgatct ccgggaaccc ggcggcgaag cgcgcctcga
     6841 gcgcgatccc cttctcttgc gccgagcgct cgaggttctc gatgatggcg cccacgaccg
     6901 cgggcagcga gaggcgctgg ggcttgaggt cgatgcgccc ggcctcgatc tttgagaggt
     6961 ccaggatgtc gttgatgagc gcgagcaggg tgcgaccgtt tcgctcgatg atctcgagat
     7021 agcccacctc gtcggccggc aggcgctccg ccgcctgacg gatcagcacc ttggacagcg
     7081 acaggatggc gttgagcggg gtgcgcagtt cgtggctcat gttcgacagg aactcgctct
     7141 tcaggcggtt cgcctcctcg acccgcgcgc gctgctcctc gagctccgcg ttctgctggt
     7201 tcacccgctc ggtctgccgc cggagctctt cggccttggc ctgcagctcc tccttctgca
     7261 tctccagcag ctggttcttg ctccggagct cgcgcgccag cacccgcgtc tcctcgttgg
     7321 cgataatgtt cgaaaacgac gtgttcatga ccggccacac gaggttgagc accttgatgg
     7381 ccatctcgga atactcgccc agcgacgcca gcgagatgat ggccacgcac cggttgttca
     7441 ccaccacggg tacggtcacc agctccgtgg gcacgacgtc gccgatcacg gtccgcagcc
     7501 tcagcatcga cgtcccggag atgttgcggg tgcgggtgat ccggcgggtc gccagcgcgc
     7561 ggccgaactc gccctcgagc ttctcggcgt gaaaggtctc catggcgttc gcgtccaggc
     7621 cgatggaggt gagatggacg aactcgcggc cgtccggcga gagcacgtag aaggcgccga
     7681 tgctcgacgc ggtcatctcg acgagcgccc ggaggacccc ggtgctgaaa tcgtgcagct
     7741 ccaccgcgga caccatgatc tcgatgatgt cggagctttc cttctggagc tggagctcgg
     7801 cggtgatcga atcggccatg gcattgaacg attcggcgag acggcccagc tcgtcccgcc
     7861 ccgggatcga cgcccgcacc gcgtactcgc cgccgccgat ccgccgcatc accgacgaga
     7921 gcgcctcgag cggcgcggca agaccgctcc ccagccgcac gccgacgtac gcaagcagcg
     7981 cgagcgcgac aacgaacgcg agcgccatct ccccgagcat gcgccgcgcc ggggcgtaca
     8041 cctcctcggc gtccttcttc acgacgagcc cccagcggag gcgcggcagg tgcgtgtacg
     8101 ccgcaagcgc cgtccgtccg cggaaatcgg tcacctccat ggcgccggtc ttcccctcgg
     8161 ccgcgagccg cgaagcggga tcatccagcc gctgcgagag cggcgcgccc tcgagaccgc
     8221 gcacccggtt catgagcttg tgccgcccgt tcaccaggag ggcctcgccg gagctcccca
     8281 ggccgccgcg ctcgcccaag agctcgtaga gcgagcgatc gaggtagatg cgcgcgacca
     8341 ggtatcccgt gaggggggcg tctccctgcg gcgaggcgtg cagcgggacc gcgacggtca
     8401 tggtggggat gctcagattc ttcgagaaat tgatgtcctt gaccaggatg tcgttgtcgc
     8461 ggatggcgtc ggagaagtac gattccgccg ggcgcgcctc gccgatgttc tcgcgcgtgg
     8521 tggaatacac gatcttcccc agccgcgcgc tgataatgaa gaactcgtaa tacagattgt
     8581 agttgtgcag ataggcgttg aacacgccgc ggaacggcgc gctcgcctgg tgcccggaga
     8641 gcgcgccgga gcccatgagc gcggcgttcg agcgaacctc cggcatcgcg gcgagcgtcc
     8701 gcaggtcgcc ggcgatccgg tccagccatt gcccgatggc gcgcgcccgc gcgtcgcgct
     8761 gcgcctcgag ccgctccgtt tccgccgcgc ggatgtccga catgcggacc agatacatcg
     8821 aaaggccgaa caccgcgaga agcgacaggg cgagtccgca gacccagaac agggccgcga
     8881 cgcgaacgct ggaaaagttc ggcgcgcgct tcagcatgcc ggatcagcga tgccgcgcga
     8941 ggcttgtttt gataagatag acgacattga agatattgct gatgccgccc acgaagcgct
     9001 ccttgatcat gcccgtgggg agcgtgtcga tcgcgtcctt gagcgacgcc accccccgct
     9061 ccacctgtgc cagctcctcg agcgcggcgt ccgcgccccg tgctgcgacg atggaatcga
     9121 cccttgcatc gatgtcgcgg atggagaaaa acatggtccg gtaggcctgt cgcgacggct
     9181 cgggcaaatc cgcaatggcc ttctccacga cgcagatgcg gaccatctgg tagccgagct
     9241 ggacctcctg gcagttgcga ttgctgaagc agtcgggata gcgctcgaag tagaccgcgc
     9301 ccagctccag catgtcgccg ggctcgaagg cgaagcgccc gcccagcccg acaatggatt
     9361 cgcggaattc cgcgatcccc cggtgctcct tgaacagatc ggcgatcagg gcgcccatac
     9421 cacctccatc gcgctgcctg atgcgcatag tcgcggaaca cccgtttttt ttcaaccaag
     9481 aaacagacgg catctcggcg cgtcccggtt acggcatcgc atttttgttg cgcaaatcgg
     9541 cgcgcatgat aggctctgtg catcgcgatt atcgcaccga tacgccgcat ccgcaggagg
     9601 agccagaccc atcatgaccg cagaccgacc cgcatcgctg gtcttcgcgc aatcgggcga
     9661 cgcctgtttc cgttatcgcc ccaccgaacg ggaatcggag gtcctcgagc gcatcaaccg
     9721 aaagatcgcc gcgctcgaca ccctcgagca gaccatcgac ttcctcttcg cggaaacgca
     9781 ggggatcatg ccctgcgacc gacttgccgt ggcgttcttc gaggacgagg ggacgcgcct
     9841 cacgatccac cacgtcgtct cccggaccga gccgcgccgc ctgacaaaag gctacagcgc
     9901 cgacatcgcg cgaagctcgc tccggcgcgt cttcgagacg gggtgcccgc gcatcatcaa
     9961 cgacatggac gaatacgcga cggcgcaccc ggacagcgag agtgcgcggc tcatcctgat
    10021 ggagggcatc cgcgcgaaca tgacctgccc gctcacggtc gacggtcggc ccgtcggcgt
    10081 tcttttccga agcgcgagcg cgcccggcgt gtatgccgac cgcgaggtcg cgctccacct
    10141 catgatcgcc gagcgcctca gccaggcggt ggagaaggcc tggcggatcg agcagctctc
    10201 gtccaccgtg aacgcctacc tggagacgct cgccttcgtg acccacgagc tcaggagccc
    10261 gctcgactcc atcgccacca tgggaaaaac gatcgtgaac ggctacctgg ggcccgtcga
    10321 tgcgcgcgtc gccgagttcg tcgggcgcat ggtgaagaag gcggagtacc tcagcggcat
    10381 cagcaacgac tacctgaacc tggcgcgttt cgagagcggc ggcgcgagcc tgcgcacggc
    10441 gacggtcgat ttcatcacgg acattgtcga tgaagcgatc gacatcatca ggccccagat
    10501 ggaggagcag tcggtccgcc tcgagaccga ctaccgcctc ggcggccgcc ctgtatcctg
    10561 cgaccccgcg ctgatgaaaa cggcgatgat caacctgctg ggcaacgccg tcaagtacgg
    10621 caaccgcgaa ggcgttgtgc gaatcacggc gaccgacgac accggcgcgc tggtcgtatc
    10681 ggtctggaac gaagggcccg ggttttccgt cgaggaccgc gcgcggctct tccggcgctt
    10741 ttcgcggctg cccaagaagg agctcatggc gcgcaagggc tcggggatcg ggctgtatgt
    10801 cacctggaaa attctgcagc tccacggcgg gcgcattcag gcgcaatcgg aggaaggccg
    10861 ctgggcggag ttcaccttcg agatcccgct cgggaacgac acggaatcaa cctgaacagg
    10921 tttatgttta tgagcacctg ccggtcgccg attcgccgca tcggtaaaat ccagttgtat
    10981 taattttttt attgattttt acccgtgaaa cacgtaccgc tgacagtatt aaaaatcaca
    11041 tggaggttgt acatgaagaa attctctctc gtactgttca tcctcgttat cgcagtgtcc
    11101 ttcgcggcga tttcgtgcag cggacaaaag aaatccggcg ccgcctcgtc cgggaacatg
    11161 gtcgtcgacg ccctcaatgc gtcgctcgcg aaggttccga tcgaaggctt tcagtccggc
    11221 aaggccgcat ccaagcagga gtgggacgcg atgatcacca aggcgatccc ggtcgtcaag
    11281 gaagtcctcc cgaaggttcc ggacggatac a
//
LOCUS       DAORHF010000109        11253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4966_length_11253_cov_7.282193, whole genome shotgun sequence.
ACCESSION   DAORHF010000109 DAORHF010000000
VERSION     DAORHF010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4966_length_11253_cov_7.282193"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PKJ16_10285"
     CDS             complement(<1..182)
                     /locus_tag="PKJ16_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HNV47418.1"
                     /translation="MGFCDSLSARTVYVTDDEKPLVVQSDFTIFLDVHSPVFREARDK
                     LMRFAELVKSPEHMHV"
     gene            complement(231..2813)
                     /gene="gyrA"
                     /locus_tag="PKJ16_10290"
     CDS             complement(231..2813)
                     /gene="gyrA"
                     /locus_tag="PKJ16_10290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HNV47419.1"
                     /translation="MTEDSGARPTERIEELDIEEELKGAYLTYAMSVIIQRALPDVRD
                     GLKPSQRRILVAMHDLGLAPRGRFLKCAKIAGDTSGNYHPHGESAIYPTLVRLAQPWN
                     VRHRLIDGQGNFGSPDGDPPAAMRYTEARLTTEAVELMEDLDKDTVEFVANFDGRLQE
                     PTVLPSKFPNLLVNGSSGIAVGMATSLPPHNLGEVIDALKAVVANPAITVREILEILP
                     GPDFPTGGIICGRGGIRSAYETGRGLITIRSRTAIEEEDGRARIAVTEVPYQLTKTKL
                     IEAIVDQIKAERITGIGDVRDESDQKGQRLVIEVKRGEDPALVLNQLYKYTPLQSTFS
                     IINIALVDGRPRTLTIKELLVAFVRHRFTVIRRRTAFLLAKAEARAHILEGLLRALAM
                     IDEIIAAIKASASPAEAGRRLMDQFGFTEPQATHIRQMQLQALTGLERERLQAEYEEL
                     VKTIARLRRIMAEDGLVYDVMVEEWEDLRKRCADARRTTFAETAEEIEDEDLIPEDRV
                     VVTITREGYLKRTSLAAYRLQGRGGIGVRGLDAREGDVVRSVFVASTHDYVLFFTNQG
                     RIYWLKAYQLPDLDRASRGRAAVNLLNLRENERVAQALCLEELSGGYLIFATRQGVVK
                     RTALEAYSRPHKTGIHAILLDEGDELIAVQRCGDDDDIMLVTRNGQANRFAAGDARPM
                     GRTAHGVRGIALRGDDRLVSLAVVREGLYLYTVCSNGYGKRTAIAEYPRHRRGGGGVR
                     DIRTSGRNGTVVAAQAVADGDHVMLITVSGQVIRAPVTDVSAIGRGTMGVRVIRLREG
                     DVVASLERISAEDLAAEAAAERNGGEEERGRAARPPRAAEHGDPFDVDDEAPSETPSS
                     EDEE"
     gene            complement(2841..5246)
                     /gene="gyrB"
                     /locus_tag="PKJ16_10295"
     CDS             complement(2841..5246)
                     /gene="gyrB"
                     /locus_tag="PKJ16_10295"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HNV47420.1"
                     /translation="MVDTYDWRSIKVLEGLEAVRKRPDMYIGSTGDDGLHHLANEILD
                     NSIDEALAGFADKIWVDIGEDGTMAIRDNGRGIPVDMHQEAGVSSLQVVLTKLHAGGK
                     FDHGSYKVSGGLHGVGLSVVSALSEWLQVEVYKDGWIYRQTYAEGVPQTPVERIGKTD
                     MRGTKIMFRPDTDIFPSVRWDYGILCKRCRELAFLNPGVRIKIRDARTDKEEEFLFAG
                     GIVSFVEYLGESRTPIHPEVIRVAGEQGTTQIDAALRYDDGYSSDTIYTYVNNIRTIH
                     GGTHESGFRSGLTRALNRYGKTANIFKDDVTPDGRDYLEGLCAVVSVKVLDPKFESQT
                     KVRLANPEVEGAVQSIVMEKLGSFFEEKPAVAKAILTKAVLAARAREAARKARELTRR
                     KGILSSGGLPGKLYDCVRRSADGTELFIVEGDSAGGSAKQGRVREYQAILPIRGKIIN
                     VEKARLDKVLNHAEVQTLISAIGAGVGSDEFDPEKVRYGKIIIMTDADVDGSHIATLL
                     LTFFFRQMRPLLEAGGVYLAQPPLYRVTHRNKERYVQKDEDLRRYIVEVGLKEVKFTR
                     SGEGAPMDTAALNRALGAVAHAESYRGSLEKKGMPLEKFLGMAKSGQLPILGVRWNGV
                     ERIFYSENEYAEFLQERQAEKGADLTIGEADGHVPEGADFTVWEFTEAVPLSRILKEL
                     TEVGFAAADYHRTDGGPPLGTAASGGEERVLKDLRELAELVREKGQGAVKYQRFKGLG
                     EMNAEELWETTMKPENRTLLRVRLEDAARADEIFTILMGTNVEPRRQFIERYARDARN
                     LDI"
     gene            complement(5277..5585)
                     /locus_tag="PKJ16_10300"
     CDS             complement(5277..5585)
                     /locus_tag="PKJ16_10300"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HNV47421.1"
                     /translation="MKSRRRDFMPIKGLINGLVRRLRPPAEDSQACVAETWARTVGEE
                     FLAIARVSGISENTVRIDVAGAAARAELESFYRARFLQALRDAGMRHLTRVYFHVRDV
                     "
     gene            complement(5582..6712)
                     /gene="dnaN"
                     /locus_tag="PKJ16_10305"
     CDS             complement(5582..6712)
                     /gene="dnaN"
                     /locus_tag="PKJ16_10305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HNV47422.1"
                     /translation="MKAVCKRSSLAEALGVITAGVPAQAPTPVARDVLISVDHEGLRL
                     ESTDLTIFSEVKVLEIKEAVSGEALTPIACIAGLVHELDKEFITIEREEGQFGLSISS
                     GNDEFKIIGHDPGDFPSSPETTQCAGFEVESAVLAEAIRLVAFAASKDPSRRQLMGVA
                     FVAEGAKAYFVASDGKRLAEYVVATAERADKRREGILPLAAVEAIAKLLPLDNGQVSI
                     KIDPDLQQAVVSHERGRVLCRLIEGQMPDYVSLIPSEFATTVEGSARELLAAVRKAAV
                     LTTRDNAFVRLEYGDGAFSVHVSSKETGSGVVPVDNVTVSGDPIVVNFAVQYLSDGLR
                     VMGDTVVRLGLQKSDGAAVVHGGRAFRYAFMPVRPRGADDEV"
     gene            7480..8052
                     /locus_tag="PKJ16_10310"
     CDS             7480..8052
                     /locus_tag="PKJ16_10310"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HNV47423.1"
                     /translation="MPDQTSKDRSPLLSATQKAAIFVPLAVGVFALDLASKLVVFRAA
                     DACIIRGSDGPYADAARVIPVIPGFFDLQCVLNAGAFSGWFRGWYWFLIIVSVAALAA
                     ITAYVLYGPVHGRLFAAALACIAGGTAGNLYDRIAYGAVRDFFHAYVTIGGRTWTWPN
                     FNVADAAICAGAGLWITLEFALSRGRRKRC"
     gene            8180..8761
                     /gene="efp"
                     /locus_tag="PKJ16_10315"
     CDS             8180..8761
                     /gene="efp"
                     /locus_tag="PKJ16_10315"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV47424.1"
                     /translation="MKVNELKKGMYVEIDKELWQILQILPVTRGNWRTYYQVSLKSLA
                     KGTVVERRHSGTDDLTYAFLEGREVEYLYEEPDSYVFMDTKTYEHLHIPAALVKDQIG
                     YVRHNSRVKMQFYGGNAVSLDLPAAVVLKVVETDPSVRGDTVKNVTKPAELETGLEIR
                     VPSHVVTNDFVKVDTRTGQFISRAAPGEIPAEE"
     gene            complement(9008..11044)
                     /gene="shc"
                     /locus_tag="PKJ16_10320"
     CDS             complement(9008..11044)
                     /gene="shc"
                     /locus_tag="PKJ16_10320"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HNV47425.1"
                     /translation="MTQKIGPLDIFKLIKPRGAAAAARPAPRRESPTLPHLPAPAEPP
                     VTDALARARDWLLSLQNDAGYWVAELEADTTVESYFILFRTFMGRHDDPRIPKYAKTI
                     RSCMLPQGGWAAYENGPPDLSASVLSYFALKVAGISADEEDMRRATAVILNLGGIANA
                     NTYTKFYLAFFGQYDWSHVPAVPPEMILLPSKIPINIYSISSWARAIFVPMSILYALK
                     PVCAIPPECGVDELIVGGRENADLSLPRPPKVFSWSTVFLLTDKALKMAERFPIALAR
                     KAAIRRAERWMLARFERSGGLGAILPGMVNSIMALRCLGYPDDHPAIAKGLRDLEALE
                     IHDPEDDSIRVQPCHSPVWDTAISTYALAVSGATIESEPRLRASVAWLLSKEVKISGD
                     WKVKNPAPPSGWYFEFENEFYPDVDDTVMVLMGLRTLHAPGVSPAVDGAIERGLQWVL
                     GMQSANGGWASFDRDNDKNFLTNIPFADFNAMIDPSTADITARVLEMFGTVAPEQYNP
                     WHPAVRRALEFIRKDQREDGSWYGRWGVNYLYGTWQVLRGLRIVGEDMRKDYVRAAVE
                     WLMSVQHADGGWGERADSYDFPNRKGKGPSTPSQTAWALMGLLAAGEGHSQAARHGLK
                     HLLDTQRRDGTWDETEWTGTGFPGVFYLRYHFYRHNWPLMALSQYRRFLRGYRP"
BASE COUNT         1834 a         3777 c         3590 g         2052 t
ORIGIN      
        1 tagacgtgca tgtgctcggg gctcttgacc aattccgcga agcgcatgag cttgtcgcgc
       61 gcctctcgga acaccgggct gtgcacgtcg aggaagatcg tgaagtcgct ctgaacgacc
      121 agcggtttct cgtcgtctgt cacgtatacc gtcctcgccg acaatgagtc gcagaatccc
      181 atcttggccc atccggggca atcattcaag tatgagactc ggccggcggg ctattcctcg
      241 tcctcggagg acggcgtttc ggacggcgcc tcatcatcga cgtcgaacgg gtcgccgtgt
      301 tcggccgcgc gcggaggccg cgcggcgcgc ccgcgctcct cttcgcctcc gttccgctcc
      361 gccgcggctt ccgcggcaag gtcctcggcg gagatgcgct ccagcgacgc gacgacgtcg
      421 ccctcccgca ggcggatcac ccggacgccc attgtgccgc ggccgatcgc cgacacatcg
      481 gtcaccggcg cgcgaatgac ctggccgctc accgtgatca gcatgacatg gtcgccgtcc
      541 gcgacggcct gcgccgcgac gaccgtgccg ttccggccgc tcgtgcggat gtcgcgcacg
      601 ccgccgccgc cgcgccgatg gcgcgggtac tcggcgatcg ccgtgcgctt gccgtatccg
      661 ttgctgcaca ccgtgtagag gtacaagccc tcccgcacca ccgccagact gacgagccgg
      721 tcgtcgcctc gcagggcgat gccccgcacg ccgtgcgcgg tgcgccccat gggccgcgca
      781 tcgcccgccg cgaaccggtt ggcttggccg ttccgcgtca cgagcatgat gtcgtcgtcg
      841 tcgccgcacc gctggaccgc gatgagttcg tcgccctcgt cgagcagaat ggcgtggatg
      901 ccggtcttgt gcggccggct gtaggcctcc agcgccgtcc gcttcacgac gccctggcgc
      961 gtggcgaaga tcagataccc gcccgagagc tcctccaggc agagggcctg cgcgacgcgc
     1021 tcgttctccc ggaggttgag caggttgacg gcggcgcggc cgcggctggc gcgatccagg
     1081 tccgggagct gatacgcctt gagccagtag atgcggccct ggttcgtgaa gaacaggaca
     1141 tagtcgtgcg tcgaggccac gaacacgctg cgcaccacat cgccttccct ggcatcgagc
     1201 ccgcggacgc cgattccgcc gcggccctga aggcggtacg ccgccaggct cgtgcgcttg
     1261 aggtatccct cgcgggtgat cgtcacgacg acgcggtcct cggggatcag gtcctcgtcc
     1321 tcgatctcct cggcggtctc cgcgaaggtc gtgcgccgcg catcggcgca gcgcttccgc
     1381 agatcctccc actcctccac catgacgtcg tagaccaagc cgtcctccgc catgatgcgg
     1441 cgcaggcggg cgatcgtctt gacgagttcc tcgtactcgg cctgcaagcg ctcgcgctcc
     1501 aggcccgtca gcgcttggag ctgcatctgc ctgatgtgcg tcgcctgcgg ctcggtgaag
     1561 ccgaactgat ccatgagccg ccggccggcc tcggccgggc tcgccgacgc tttgatcgcg
     1621 gcgatgatct cgtcgatcat ggcgagggcg cgcagcagcc cctcgaggat gtgcgcccgg
     1681 gcctcggctt tcgccagcag gaaggcggtg cggcggcgga tgaccgtgaa gcggtggcgc
     1741 acaaaggcga cgagcaattc cttgatggtc agggtccgcg gccgtccgtc caccagggcg
     1801 atgttgataa tgctgaacgt cgactggagc ggcgtgtact tgtagagctg gttcaggacc
     1861 aaagcggggt cctcgccccg cttcacctcg atgaccaggc gctggccctt ctgatcgctc
     1921 tcgtcgcgca catcgccgat gcccgtaatg cgctcggcct tgatctgatc gacgatggcc
     1981 tcgatgagct tggtcttcgt gagctggtag ggcacctcgg tgacggcgat gcgcgcccgg
     2041 ccgtcctcct cctcgatggc cgtccgggag cggatcgtga taagcccgcg gcccgtctcg
     2101 taggcgctgc ggatgccccc ccgaccgcag atgatgcctc ccgtgggaaa atccggcccc
     2161 ggcagaattt ccaggatctc gcgcacggtg atcgccgggt tcgcgacgac cgccttcagg
     2221 gcatcgatga cctcgccgag gttgtgcggc gggaggctcg tcgccatgcc gacggcgatg
     2281 cccgagctgc cgttcaccag caggttggga aacttgctcg gcagcaccgt cggctcttgg
     2341 agccggccgt cgaagttggc cacgaactcc acggtgtcct tgtcgagatc ctccatgagc
     2401 tcgacggctt cggtggtgag gcgcgcctcg gtatagcgca tggccgcggg cgggtctccg
     2461 tccggcgagc cgaagttgcc ttgcccgtcg atcaggcggt ggcgcacgtt ccaaggctgc
     2521 gccaggcgca ccaaggtggg atagatggcg ctctcgccgt gggggtggta gttgcccgac
     2581 gtgtcgccgg cgatcttcgc gcacttcagg aagcggcccc gcggggcgag gcccaggtcg
     2641 tgcatcgcca ccaggatgcg ccgctgcgac ggtttcaggc cgtcgcgcac atcggggagc
     2701 gcgcgctgga tgatcacgct catggcgtag gtgaggtacg cgcccttgag ctcctcttcg
     2761 atgtccagct cttcgattcg ttcggtcggc cgtgcgccgg agtcttcggt catggtgttt
     2821 ttcccgccgc gggcgggttc ctatatgtcg aggtttctcg catcgcgggc atagcgctcg
     2881 atgaactgcc gccgcggctc cacgttggtg cccatgagaa tcgtgaagat ctcatcggcg
     2941 cgcgccgcat cctccaggcg cacccggagc aaggtccgat tctcgggctt catggtggtt
     3001 tcccacaact cctcggcgtt catctcgccc aggcccttga agcgctggta cttcaccgcg
     3061 ccctggcctt tctcccgcac gagctccgcc agctcgcgca ggtccttcag gacgcgctcc
     3121 tctccgcccg aggccgcggt ccccaacggc ggaccgccgt ccgtccggtg ataatcggcg
     3181 gccgcgaacc ccacctcggt cagctccttg agaatgcggc tcagcggcac cgcctcggtg
     3241 aactcccaaa cggtgaaatc cgcgccctcg ggcacgtggc cgtcggcctc gccgatggtg
     3301 agatccgcgc ccttctcggc ctggcgctcc tggaggaact ccgcgtactc gttctcgctg
     3361 tagaagatcc gctccacccc gttccagcgt acgcccagga tcgggagttg gccgctcttg
     3421 gccataccga ggaacttctc cagcggcatg cccttcttct ccagcgagcc gcggtagctc
     3481 tcggcgtggg cgacggcgcc cagcgcgcga ttgagcgcgg cggtatccat gggggcgccc
     3541 tccccgctgc gggtgaactt cacctccttg agccccacct cgacgatgta gcgccgcaga
     3601 tcctcgtcct tttgcacgta gcgctccttg ttgcggtggg tgacgcggta caagggcggc
     3661 tgcgccaggt acacaccgcc ggcctccagc agcggccgca tctgccggaa gaagaacgtc
     3721 agaagcagcg tcgcgatgtg ggaaccatcg acgtcggcat cggtcatgat gattattttg
     3781 ccgtacctga ccttctcggg atcgaactcg tcgctcccga cgcccgcgcc gatggcggag
     3841 atgagggttt gcacctcggc gtggttgagc accttgtcca ggcgcgcctt ctcgacgttg
     3901 atgattttgc cgcgaatcgg aaggatcgcc tggtactccc gcacgcgacc ttgcttggcc
     3961 gagccgccgg ccgagtcgcc ctcgacgatg aagagctcgg tgccgtcggc cgagcgccgc
     4021 acgcaatcgt agagtttgcc gggcaggccg ccgctggaga ggatgccctt gcggcgcgtg
     4081 agttccctgg ccttgcgcgc ggcctcgcgg gcccgcgccg cgagcacggc tttcgtgagg
     4141 atcgccttgg cgaccgccgg cttctcctcg aagaacgagc cgagcttctc catgacgatg
     4201 gactgaaccg cgccctcgac ctcggggttc gccaggcgca ccttggtttg gctctcgaat
     4261 ttcgggtcga ggaccttcac gctcaccacg gcgcagagcc cctcgaggta gtcgcgtccg
     4321 tcgggcgtga cgtcgtcctt gaagatattc gcggtcttgc cgtagcggtt gagggctcgc
     4381 gtcaggcccg agcggaagcc gctctcgtgg gtgccgccgt ggatggtgcg gatgttgttg
     4441 acgtaggtgt agatcgtgtc gctggagtac ccgtcgtcgt atcgcagcgc cgcatcgatc
     4501 tgcgtcgtgc cctgctcgcc cgcgacgcgg atcacttccg ggtggatcgg cgtccggctc
     4561 tcgccgaggt actcgacgaa ggaaacgatg ccgccggcga aaaggaactc ctcctccttg
     4621 tccgtgcgcg cgtcgcgaat cttgatgcgc acgccgggat tgaggaacgc gagttcgcgg
     4681 cagcgcttgc agagaattcc gtagtcccaa cgcacgctcg ggaaaatatc cgtgtcggga
     4741 cggaacatga tcttggtgcc gcgcatgtcc gttttgccaa tgcgctcgac gggagtctgg
     4801 ggcacccctt ccgcgtaggt ttggcggtaa atccagccgt ccttgtagac ctcgacctgg
     4861 agccattcgc tcaaggcgct caccacggac aggcccaccc cgtggaggcc gcccgacacc
     4921 ttgtaggaac cgtggtcgaa cttgccgccg gcgtgcagtt tcgtcagaac cacctgcagc
     4981 gaggacacgc ccgcctcttg gtgcatatcg acggggatac cccggccgtt gtcgcggatg
     5041 gccatcgtgc cgtcctcgcc gatatcgacc cagatcttgt ccgcgaagcc ggccagggct
     5101 tcatcgatgg agttgtcgag aatctcgttg gcgaggtgat gcaagccgtc gtcgcccgtg
     5161 ctgccgatat acatgtccgg ccgcttgcgc accgcctcca ggccctcgag aaccttgatg
     5221 cttctccagt cgtaggtgtc taccatattc gccgccatcc cgaattccgc ttcccctcag
     5281 acatcgcgca cgtgaaaata cacccgtgtc aaatgtctca tacccgcatc gcgaagcgcc
     5341 tggaggaacc gagcgcggta aaacgactcc agttccgccc gcgcggccgc gccggcgaca
     5401 tcgatcctga cggtgttctc cgatattccg cttacgcggg cgatggccag aaattcctcg
     5461 ccgacggtgc gcgcccaggt ctcggccacg cacgcctgcg agtcttccgc cggcggacgg
     5521 agccttcgta ccaagccgtt gatgaggccc ttgatcggca tgaaatcacg ccgcctgctt
     5581 ttcatacctc gtcgtccgct ccccttggtc taaccggcat gaacgcatac ctgaacgcgc
     5641 gcccgccgtg caccaccgcg gcgccgtcgc tcttctgcaa tcccaaccta acgaccgtgt
     5701 cgcccatcac acgaagccca tcgctcagat actgcacggc gaagttcaca acgatgggat
     5761 ccccgcttac cgtcacgttg tcgacgggca ccacgcccga gcccgtctcc ttggaagaga
     5821 cgtggacgga gaaggcgccg tcgccgtatt ccagcctcac aaaagcgttg tcccgcgtcg
     5881 tcagaaccgc cgccttcctc accgccgcga gcagttcgcg cgccgagcct tccaccgtcg
     5941 tcgcgaactc gctcggtatc agcgaaacgt aatcgggcat ctgcccctcg atgagccggc
     6001 acagaacccg gccgcgttcg tgggacacga ccgcctgttg aagatcagga tcgattttga
     6061 tgctcacctg gccgttgtcc aacggcagga gcttggcgat ggcctccacc gcggccaacg
     6121 gcaagattcc ctcccggcgc ttgtccgctc gctccgcggt ggccacgacg tattcggcca
     6181 agcgcttccc gtcggacgcg acgaagtacg ctttggcccc ctcggcgacg aatgcgactc
     6241 ccatgagctg ccggcgcgat ggatccttcg acgccgcaaa agcaaccagc cgaatcgcct
     6301 cggccaacac ggcgctctcc acttcgaaac ccgcgcactg cgtggtttcc gggctgctgg
     6361 gaaaatcgcc gggatcgtgc cctatgatct tgaactcgtc gttgcccgac gagatactca
     6421 ggccgaactg cccctcctcg cgctcgatgg tgatgaactc cttgtccaac tcgtgcacca
     6481 gccccgcgat gcaagcgatc ggcgtcaaag cctcgccgct caccgcctcc ttgatctcga
     6541 gcaccttgac ctcgctgaag attgtcaaat ccgtgctttc cagccgcagg ccttcgtggt
     6601 ccaccgagat gaggacatcg cgagccacgg gcgtcggggc ttgcgctggg acccccgccg
     6661 taatgacgcc cagcgcctcg gccagactgc ttcgcttgca caccgctttc atcacacccc
     6721 cctcgtacac ctctccgcgc ctttgaaacc cgtctgaacc accgtctccg cctcacttct
     6781 tttcgctaat tcgacccgcg cgccgcgcct tcggtcgatc gcttcgccgc ccaccggcag
     6841 gctctcgccg ctctcccccg cgaagtcgat cgcaacctcg cgcgagcagc gaaagatctc
     6901 attgtatact atatttcggt aagaggcaaa ggagtctgtg ctcttctcga gagacctttg
     6961 ccaggtgttt tctgattaat gaaaacgctg ggcttctacg ctcctcagtc gcggtggagg
     7021 tagagatgga aaagagaatt agtattttcc tagtaactct catcggcttg gaagaaactg
     7081 tggaaaactt agcgggtcta taacgtaatt tattgaaata gaatgagtta tgtaattaca
     7141 acggccgctt gcggttgttg aaaacagggc cggtaatcgg tggattaatc cggctttgtg
     7201 ccgcgttttg aagaagcggg tgattccgcg tgcggaattc caccggaagc gatggagatg
     7261 atgaaagcgt catctagacc tttccaaggg gttttcaaca tgaagccgcg gggaaggacg
     7321 gactctccgg cgcttcaggc tggaagatcg agggctttcc ggcggggtaa atcggcgggg
     7381 cggcttctgg cgcttagatc ggcatgatag aataacacgc gccgcggctc gtggcaggag
     7441 gcgcgcggcg acggctcgct ggacagacaa ggctcgataa tgcccgacca gacctccaag
     7501 gaccggagcc cgcttctctc ggcgacgcag aaggccgcca tcttcgtgcc cctcgccgtc
     7561 ggcgtattcg cgctcgatct ggcgagcaag ttagtcgtgt tccgcgccgc ggatgcgtgc
     7621 atcatccgcg ggtctgatgg cccctacgcc gatgccgccc gcgttattcc cgtgatcccg
     7681 gggttcttcg acttgcaatg cgttctgaac gccggagcgt tcagcggttg gttcaggggt
     7741 tggtactggt tcctgatcat cgtgtccgtc gccgcgctgg cggcgatcac ggcgtacgtt
     7801 ttgtacgggc cggtgcacgg acgcctcttt gccgcggcgc tggcgtgcat cgccggcggg
     7861 accgcgggca acctgtacga tcgcatcgcg tatggagcgg tgcgggattt tttccacgcc
     7921 tatgtcacga tcggcgggcg cacatggacg tggccgaact tcaacgtggc ggatgcggcc
     7981 atctgcgcgg gggccggcct ctggattacg ctggagttcg cgctgtcgcg cggccgccgc
     8041 aaacgctgtt gatcgacgcg ccgcgttgat gcgttcacct tgccgccggg gggaagcgtt
     8101 gctataatcg ctccggtatg ccgcgacgac ccgcccggcg ccgaggcgcg aacatccccc
     8161 gcagacgcgc gagagagcca tgaaagtcaa cgaactcaag aaaggcatgt acgtcgaaat
     8221 cgacaaagag ctctggcaga tcctccaaat actgccggtc acgcggggca actggcggac
     8281 ctattaccaa gtatccctga aaagcttggc gaagggcacg gtggtcgaac gccgccacag
     8341 cggaaccgat gacctcacgt atgctttcct cgaggggcgc gaagtggaat atctctacga
     8401 ggaacccgac agctacgtgt ttatggacac gaagacgtac gaacacttgc acatccccgc
     8461 ggccctggtg aaggaccaga tcggatacgt ccggcacaac agcagggtca aaatgcaatt
     8521 ctacgggggc aacgccgtct cgctcgacct gcccgccgcg gtggttctca aggtcgtcga
     8581 gaccgacccg agcgtccggg gcgacacggt caagaacgtc actaagccgg cggagctcga
     8641 gacgggcctg gagatccgcg tcccctcgca cgtggtcacg aacgacttcg tcaaggtgga
     8701 cacgcgcacg ggccagttca tcagccgcgc cgcaccgggc gagatccccg ccgaggagtg
     8761 agccgctccc ttcgtctagc cgtggattcc cacggcgcct tatgcgctta acaccgcgcg
     8821 ttcgaggggc gggcataagc gctttccgcc aagccggcgg atccggacgc cggtttcgtg
     8881 gaacccggca gcggtaagaa gcgggttgcg cgcgggtttc acggaaaggc gcgatgctct
     8941 ccctttccgt gatttccacc gcggcggcgg cgccgatcag ccctgcctcc ggccgtggga
     9001 atccaggcta gggacggtag ccgcgcagga agcgcctgta ctgcgacagc gccatcaacg
     9061 gccagttgtg ccggtagaag tgatagcgca gatagaacac acccggaaat cccgtgccgg
     9121 tccattccgt ctcgtcccag gtgccgtcgc ggcgctgcgt gtcgagcagg tgtttcaacc
     9181 catgccgcgc ggcctggctg tgtccctctc cggcggccag aagccccatt agggcccagg
     9241 ctgtctgaga gggcgtcgac ggccccttgc ccttgcggtt cgggaaatcg tacgagtccg
     9301 cccgctcgcc ccagccgccg tcggcgtgct ggacgctcat gagccactcc accgccgcgc
     9361 ggacgtagtc cttgcgcatg tcctcgccga cgatcctgag gccgcgcaac acctgccagg
     9421 tgccgtaaag gtagttgacg ccccagcggc cgtaccacga gccgtcctcg cgctggtcct
     9481 tgcggatgaa ctccagcgcg cgccgcacgg cgggatgcca aggattgtac tgctccggcg
     9541 ccacggtccc gaacatctcc aagacgcgcg ccgtgatatc ggccgtgctc gggtcgatca
     9601 tggcgttgaa gtccgcaaag gggatgttcg tgaggaagtt cttgtcgttg tcgcggtcga
     9661 agctggccca accgccgttg gcgctctgca tgccgaggac ccactgcagg ccgcgctcga
     9721 tcgcgccgtc caccgccggc gacacgcccg gggcgtgaag cgtccgcagg cccatcagca
     9781 ccatgaccgt gtcgtcgaca tcgggataga actcgttctc gaactcgaag taccagccgc
     9841 tgggcggcgc cggattcttg accttccagt cgcccgagat cttgacctcc ttggacagga
     9901 gccaggccac cgacgcccgc agacgcggct ccgactcgat cgtggcgccg gacaccgcaa
     9961 gcgcgtaggt cgaaatcgcg gtgtcccaca cgggcgagtg gcagggctgc acgcggatcg
    10021 agtcatcctc cggatcgtga atctccaggg cctccaggtc ccgcaggccc ttggcgatgg
    10081 ccgggtggtc gtccgggtac ccgagacagc gcagcgccat gatgctgttg accatgccgg
    10141 gaaggatggc gcccaagccg cccgaacgct cgaagcgcgc cagcatccag cgctccgcgc
    10201 gcctgatcgc cgccttgcgc gccagggcga tgggaaaccg ctcggccatc ttcagggcct
    10261 tatcggtgag caggaagacc gtgctccacg aaaacacctt cggcggccgc ggcagggaca
    10321 agtcggcgtt ctcccgtccg ccgacgataa gctcgtcgac gccgcactcg ggcggaatcg
    10381 cgcagacggg cttcaaggcg tagaggatcg acatgggcac gaaaatcgcg cgggcccacg
    10441 atgagatcga gtagatgttg atcgggatct tggaaggcag gaggatcatc tcggggggca
    10501 ccgccggcac gtgcgaccag tcgtactggc cgaagaacgc caggtaaaac ttcgtgtagg
    10561 tgttggcgtt ggcgatgccg ccgagattca ggatgaccgc ggtggcgcgg cgcatgtcct
    10621 cctcgtccgc ggagatgccc gcgaccttca gcgcgaaata gctcaggacc gacgcggaga
    10681 gatcgggcgg gccgttctcg taggcggccc agccgccctg gggcagcatg cagcttcgga
    10741 tcgtcttggc atacttgggg atgcgcgggt cgtcgtgccg gcccatgaag gtccggaaga
    10801 ggatgaaata gctctcgacc gtggtgtcgg cctcgagctc cgcgacccag taccccgcgt
    10861 cgttctggag cgagagcagc cagtcgcgcg cacgcgccag ggcgtccgtc acgggcggct
    10921 cggcgggagc cggaagatgc ggaagcgtcg ggctctcgcg tcggggcgcc ggtcgcgcgg
    10981 ccgccgcggc cccgcggggc ttgatcagct tgaaaatgtc gagcggtccg atcttctgtg
    11041 tcatagggca catctcctct tcgggccgag gtgcattcta accgcaccca tgaaatatgg
    11101 agaaagaaga tactcgatat tgcagatcga tgcaagcgcg cggaggcggc ccgcggccgc
    11161 ttcgcgccgc cggcgcgggc gaatcagggc gccgcgcggt tcggccgcgc gcggaggccg
    11221 cgcggcgcgc ccgcgctcct cttcgcctcc gtt
//
LOCUS       DAORHF010000110        11214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_4996_length_11214_cov_6.115960, whole genome shotgun sequence.
ACCESSION   DAORHF010000110 DAORHF010000000
VERSION     DAORHF010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4996_length_11214_cov_6.115960"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="PKJ16_10325"
     CDS             complement(<1..561)
                     /locus_tag="PKJ16_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV47426.1"
                     /translation="MGSIAYNLADRVAVITGGSRGIGREIARSFLAEGARVVICGRKQ
                     DGLDGAAADLGGGDRLLTVPAHIAKEADVAALFEHTLQRFGRVDVLVNNVGMNLMTGS
                     LVDVDLVQWQKIIESNLTGTFLCSRAAGKIMRDQRKGKIVSLSSIAGRKASPAMNIYG
                     IAKAGIEMMTRNLAAELAPFNVQVNAV"
     gene            869..2329
                     /locus_tag="PKJ16_10330"
     CDS             869..2329
                     /locus_tag="PKJ16_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HNV47427.1"
                     /translation="MDTNNRPDQQNEEEDFAALFERSFVKRDNFSVGDRVSGTVSLVT
                     QETVFVDISGKSEAAVSASEFLDNDGKITLKRGDTLDAYIVEFKKGEILLTTCIGRGS
                     ASPGLLHTAQSSKIPVEGTVQGVVKGGYSVSVGGLPCFCPFSQIDVKSPANPESLIGR
                     RLQFRITQFGEKGRNIVLSRRTLIEETRQEQEERLRETLREGDVITGTVVSAAEFGIF
                     VDIGGVEALVPKSELSWARAASPRDFTVGSAVTVKVLSIDWDAKKFLLSIKQLSPEPW
                     ERISNYTEGQSVTGRIVNIIRSGAFVELEPGLEGYLPVSRMSLTKRVEKPEDAVSAGA
                     TVTVRISEINRKDRKILLDLVTGEADPWLTPAAELEGSVHTGTIESIMRSGLIVRLPN
                     GMTGMVPGSELLAPKGADLQKAYAVGSDINVAVIEANTGERKLRLSEQKAVTLKEREE
                     LREFMSKEQSAEGANTIGSLFKDRLEGLKKNLEKKA"
     gene            2326..2670
                     /locus_tag="PKJ16_10335"
     CDS             2326..2670
                     /locus_tag="PKJ16_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47428.1"
                     /translation="MIKLKTGREVQYNQNFDLFFQSLLAAVIQESRRVAAAQRTDAPE
                     PASNDEAVLREIMDNSIYIAHQIMELKERDENLARFLVTGCLFNCAVLTLPHLPADTG
                     DAGQSAGEDNIH"
     gene            complement(2667..4523)
                     /gene="recD"
                     /locus_tag="PKJ16_10340"
     CDS             complement(2667..4523)
                     /gene="recD"
                     /locus_tag="PKJ16_10340"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HNV47429.1"
                     /translation="MASRRNESVDIRALDCWRDIDRHFAAFVERAAGGSDEAVGLGAL
                     LASRRLADGDICIDLNAMAGVPLGDAVDDCPREAATVALPRAAAWVRALRSSPAVGKP
                     GEFRPLVLDEAGRLYLHRLYEYERIIRERIAERLAAPPLDFDEGLLRAGLDRLFPAPA
                     PEGLDWQRVAAASAVMRRLCVISGGPGTGKTATVVRILILLAEQARASGNEFTVALAA
                     PTGRAAARLRQSIRTWMETSGLSGEEREMLRSGATTLHRLLGLGGARPFDPARILPQQ
                     VVVVDEASMADCALMSHLLQAVPLESRLILLGDRDQLASVEAGAVLGDICDTGRAHGY
                     SAAFAERIAPVIGRCAHDVPIAAEPPIADSIIILRKSYRFDESGGIGAVSRAVREGDA
                     ARALQLVDADGGRSVRFVTDERPSASSERFSSRIVEGLQPALVAPDPTAALDSLTRFA
                     VLCALRRGPSGAEAVNRIIERVLAAQGLINPDGRWYVNRTLVVSRNSESAGLSNGDIG
                     IVRERPGVPGEAAVYFSDGEGGTRAFPPLRLPEHETAFATTVHKAQGSEFDTVIVVLP
                     ERPTRVLTRELVYTALTRARTHVELWGDREVFAAAVGNPTRRMSGLHDALWG"
     gene            complement(4510..8109)
                     /gene="recB"
                     /locus_tag="PKJ16_10345"
     CDS             complement(4510..8109)
                     /gene="recB"
                     /locus_tag="PKJ16_10345"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HNV47430.1"
                     /translation="MRPLDITACPLDGVRVIEASAGTGKTHAITGLYLRLLLERALTV
                     DSLLVVTYTVAAAEELRERIRARLRDAHAMLARRLPPDPADALMAALAARYADSEGAR
                     IALEVAYHDLDKAAIHTIHGFCRLVLSENAFESGSLFDTGLVADRSDLLAAVAADYWR
                     TSFYNLPGPIARELARRSRGPGHFAQLYRLRPLDRSFDILPRVDAPDHADIVDTLSKL
                     DAAFAELRSAWERDSDGVRELLAGAPGLNRQKYQARHLESWYAQLDAFCAAGDGRGEC
                     DPLVRFSRRVIAGAMRQGHDAPEHEFFVRCEELLALREAADGLLERFFRRHEAAFFAY
                     ADRELARRMASRNERSFDDIITDTHAAVTGGGGLAAAVRARFRAALIDEYQDTDQVQY
                     DIFRAAFDAPGSALFLIGDPKQSIYRFRGADVFSFIRAKRDAGGDDAAFTLLANRRSD
                     PALIRAVNAIFSRVANPFVIDGIGFSPAEAGENPRREAMTLGGRPGAAFEFWSLDDPE
                     ERGTVPRGRALPMLGRAVATEIVRLLDDGAPDAARIGTRPVRPGDIAILVHSNAHARV
                     MRDHLTAFGVPGVLYGAESVFSTREALELMRCLMAIALPGPEHRFRAALSTDMIGLSG
                     PDLFGLAEDEARWERFARAFSRARERWMRDGFYCAIRAFMDELEVRPRLLAFPDGERR
                     LTNLIHLAELGHGAERDRHLRPEGVVAWLGARIVSPGTDDAQQLRLETDDAAVKILTV
                     HGSKGLQFPIVFCPCLWRASDIKPGKPYLYRDPKDFSPTVDLGSGDDAVRRRAEREVL
                     SENVRLLYVALTRAIHRCYFAWGNIKGAETAAPSYLLYAPDELDPADCVGALEGHVKS
                     LPAGERASFRSALAERSGGAIVVRPVPHENAPPLRAATAPPGGIVPRPFTGSIGAHWT
                     VTSYSALAASGRESGERPDYDAHFAPFEPDAAPVAFDDIARFPRGARPGQCIHEMFER
                     VDFTDETGFEGVIDAALARHGIDAAWSAACSTMLARVCAAPLGDDGVSLRTVGRERRL
                     SELEYYFPLSAVEPAGVAEAFAVHAANPVVRNFSSAAAGLGFAGIRGFVRGFIDLVVE
                     HAGRYSIIDWKSNWLGDAPAAYTPDALAADMVRHAYVLQYHLYAVALHRYLSLRLPGY
                     EYDRNFGGVRYVYVRGVDPSGAPGCGVFADRPPRALIEALTRYLTRGDGDGVA"
     gene            complement(8106..>11214)
                     /gene="recC"
                     /locus_tag="PKJ16_10350"
     CDS             complement(8106..>11214)
                     /gene="recC"
                     /locus_tag="PKJ16_10350"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HNV47431.1"
                     /translation="SLQLARRLGVWANFRFLLPNEMVNALIGAALPGAPNERLFNTDI
                     MTWRVMRLLPECAPDPAFAAIARYLGDDPAGLKRYQLAARIVDVFDQYLTYRPELILR
                     WEGGADDDWQARLWRLLADGMRFSNPPRLQRDFFARLATPDDAMRAAVPGRISVFGVS
                     SLPRFHLDVLRAASALAEITLYQLNPSPHYWLDLAPGRSIARRLGADGSATARDSHLE
                     RGNALLASMGRQGQDFLGLLFAGDPDDRYAGDDPGEDTLLHRIQHDIYAVVDRGDAPG
                     GGDDDAARAVVAADGTVVVNACHSALREIEVLRDFVLDCFERLPGLTPGDIVVLAPDI
                     EAYAPFAQAVFSGSGDGAPHVPISIVDRPLRATSPAIAALFALLELPEGRFEAGAVMG
                     LLACPDIRRRRGLAEGDIDLLRAWIEGTRTTWGIDAAHRATLGLPATPGNTWAAGIDR
                     LIAGYAMLAAGDERFGGVLPYDPIAGDAAVALGRLLSFLEALRTLHETLCRPKPLAEW
                     RVVLEGALDDFFDPDEESARDLDAVRVFAARLPEVATGSDVDGTVSARLLAAFMADAI
                     GASRGGGEFITGRLTFCALMPMRSVPFRVVCMIGMNDASFPRRDVPAGFNRIADEFRP
                     GDRSLRDEDRYLFLESILSARDRLYISYCGQGVQDNRPIPPSIVVSELVDYIEQGHPE
                     CKPLVTRHPLQAFSPRYFRDDGALYTFSETQSAAARALLSRTAPPSFFPGPLIALPTE
                     EREIALDTLAGFFTNPARALLEKRLGVRYGPIAGELRDEEPFTLEGIDAWRVNRHVLE
                     GVLRGDSLDAIVDGLAARGELPHGGIGRGMVDALARGASAFHRALLPFLPEGDPPPRP
                     FSLTIGAYRIVGAVPVPGPRGLTTYRYARCRASDRLRAWIAHCIMLLLDPASSGVSHF
                     ISLREKKDEIARFEMDAFDDAGGYLAGLMDLYDAGMRGPLPFFPRSSLVYAEEAARGK
                     LSEPDARARARDAWLGSEFARGDADDEYVARLFSGADPIDARFAECARHVFMPMLAHQ
                     SEAKP"
BASE COUNT         1984 a         3832 c         3815 g         1583 t
ORIGIN      
        1 caccgcgttc acctgcacgt tgaagggcgc gagctcggcg gcgaggttgc gcgtcatcat
       61 ctcgatgccg gccttggcga tgccgtagat gttcatggcc ggggaggcct tgcgcccggc
      121 gatcgacgaa aggctcacga tttttccctt tcgctggtcc cgcatgatct tcccggcggc
      181 gcgggagcac aggaaggtcc ccgtgagatt gctctcgatg atcttctgcc actggaccag
      241 gtcgacatcg accagcgagc cggtcatgag gttcatcccc acgttgttca cgagcacatc
      301 gacccgcccg aagcgctgca gcgtatgctc gaagagcgcg gccacgtcgg cctccttcgc
      361 gatgtgcgcg gggaccgtca ggaggcgatc gccgcccccg aggtccgcgg ccgcgccgtc
      421 aaggccgtcc tgcttccgtc cgcagatgac cacccgcgcg ccctcggcaa gaaagctccg
      481 tgcgatctcc ctgccgatcc cccgggaccc gccggttatc acggccaccc ggtccgcgag
      541 gttgtatgca atcgacccca tcagcctgct ccttttcccg tttcacggtt cgatcgggta
      601 cacgctatac cccggcgttg ttttacgggc aagcacaatt tggcttcatc acgaaacaat
      661 attcaccgtc cccacagcgc gaccggcgcg ggcgaagccg gtccgcgact gatgatccgc
      721 gaggatcgcg cgtgaaaaat tctattgaca gaatatgcga cggcccctaa atagacacgg
      781 ttttttcgac cttccgaccc gtcagtgcgc agacgcggct ttacgacccg gaacgaaacg
      841 aacatcagcg accccccagg tgaacaacat ggacacgaac aataggccgg accagcagaa
      901 cgaggaggag gacttcgcgg cgctgtttga acgctccttc gtcaagcgcg ataatttctc
      961 ggtcggcgat cgggtgagcg gcacggtgag cctcgtcacg caggagacgg ttttcgtgga
     1021 catatcggga aagagcgaag cggccgtgag cgcgagcgag tttctcgaca acgacggaaa
     1081 aatcaccctc aaacggggcg acaccctcga cgcatacatc gtggaattca agaaggggga
     1141 gatcctgctt accacctgca tcgggcgcgg gtcggccagc ccgggcctgc tgcacacggc
     1201 ccaatcgtcg aagatccccg tcgagggcac cgtgcagggc gtggtgaagg gcggatacag
     1261 cgtgtcggtc gggggccttc cctgcttctg tccgttctcg cagatcgacg tcaagtcgcc
     1321 cgcaaacccc gaatcgctca tcggccgtcg gctgcagttc cgcatcacgc aattcggcga
     1381 gaagggacgc aatatcgtcc tttcgcgccg caccctgatc gaagagacgc gccaggagca
     1441 ggaagagcgc ctccgggaaa cgctgcgcga gggcgatgtc atcacgggga ccgtcgtttc
     1501 ggcggccgaa ttcgggatct tcgtggacat cggcggcgtc gaggcgctgg ttcccaaatc
     1561 ggagctctcg tgggcgcgcg ccgcgtctcc ccgcgatttt accgttggaa gcgccgtgac
     1621 cgtgaaggtc ctttcgatag actgggacgc caagaaattc cttttaagca ttaagcaact
     1681 ctcgccggag ccgtgggagc ggatcagcaa ttacacggaa gggcagtcgg tgaccggaag
     1741 gatcgtcaac atcatccgga gcggcgcctt cgtggagctg gagccgggac tcgagggata
     1801 tctgcccgtc tcgcgcatga gcctcaccaa gcgcgtcgaa aaaccggagg acgcggtgag
     1861 cgcgggcgcg accgtcaccg tgcgcatcag cgagatcaac cggaaggatc gcaagatcct
     1921 tctggacctc gtgaccggcg aggccgaccc gtggcttacc cccgcagcgg aactcgaggg
     1981 aagcgtgcat acgggcacca tcgaatcgat tatgcggtcc ggcctcatcg tgcgcctccc
     2041 caacggcatg accggcatgg tccccggcag cgagcttttg gcgcccaagg gcgccgacct
     2101 ccagaaggcc tatgcggtgg ggagcgatat caacgtggcc gtgatcgagg cgaatacggg
     2161 cgagcggaaa cttcgcctga gcgagcagaa ggccgtcacg ctcaaggagc gcgaggagct
     2221 ccgcgagttc atgagcaagg agcaatcggc ggaaggggcc aacaccatcg gctccctctt
     2281 caaggaccgc ctcgagggac tcaagaaaaa cctggaaaag aaggcatgat caagctgaag
     2341 accggcaggg aggtccagta caatcaaaac ttcgacctct tcttccagag ccttcttgcc
     2401 gccgtcattc aggaatcgcg ccgggtcgca gccgcccagc gaacggacgc cccggaaccg
     2461 gcctccaacg acgaggcggt cctccgcgag atcatggaca atagcattta catcgcccac
     2521 cagatcatgg agctgaagga gcgcgacgaa aacctcgcgc gctttctcgt gaccggatgc
     2581 ctcttcaact gcgcggtgct caccctcccc catctccctg cggacaccgg cgacgccggt
     2641 cagagcgccg gcgaggacaa catccactag ccccagagcg cgtcgtgcag ccccgacatt
     2701 cggcgcgtcg ggtttcccac cgccgccgcg aagacctccc gatcgcccca gagctcgacg
     2761 tgcgtgcgcg cgcgcgtgag cgcggtgtac accagctcgc gcgtgagcac ccgcgtcggg
     2821 cgctcgggca ggacgacgat cacggtatcg aactcggacc cctgcgcctt gtgcaccgtg
     2881 gtcgcgaagg ccgtttcgtg ctcgggcagc cgaagcggcg ggaacgcgcg agtgccgccc
     2941 tcgccgtccg aaaaatatac cgccgcctcg ccgggcaccc cggggcgctc cctgacgatc
     3001 ccgatatcgc cgtttgaaag cccggccgac tcgctgttgc gcgacacgac cagggtccgg
     3061 ttcacatacc accgcccatc gggattgatg agtccctgtg cggccagcac gcgctcgatg
     3121 atccgattga cggcctccgc ccccgaggga ccgcgccgga gcgcgcagag caccgcaaag
     3181 cgggtgagcg aatcgagcgc cgcggtagga tccggcgcga caagcgccgg ctgcaggccc
     3241 tcgacgatcc gcgaggagaa gcgctccgag gaggccgacg gacgctcgtc ggtgacgaac
     3301 cggacgctcc ggccgccgtc cgcatcgacc agttgcaggg cgcgcgcggc gtccccctcc
     3361 cggaccgcgc ggctcacggc gccgatcccg ccggactcat cgaaccggta gctttttcgg
     3421 aggatgatga tggaatccgc gatcggcggt tccgccgcga tcggcacgtc atgcgcgcat
     3481 cgaccgatga ccggcgcgat ccgctcggcg aacgctgccg aatacccgtg cgcccgtccc
     3541 gtatcgcaga tgtcgcccag caccgcgccg gcctcgacgg acgccagctg atcgcggtcc
     3601 cccagcagaa tgagccgcga ctcgagcggg accgcctgca gcagatggga catgagcgcg
     3661 cagtcggcca tcgaggcttc gtccaccacc acgacctgct gcgggaggat gcgcgcgggg
     3721 tcgaacggtc gcgcgccgcc cagcccgagc agtcgatgga gcgtcgtcgc gccgcttcgc
     3781 agcatctcgc gctcctctcc ggagagcccg ctcgtttcca tccaggtccg gatcgattgg
     3841 cgcaatcgcg cggcggcccg gccggtgggg gcggcgaggg caacggtgaa ctcgtttccg
     3901 gacgcgcgcg cctgctcggc gagaaggatg aggatgcgca ccaccgtcgc ggtctttccg
     3961 gtcccgggcc cgccggatat cacgcacagc cggcgcatca cggcgcttgc ggccgcgacc
     4021 cgctgccagt cgaggccctc cggcgcgggc gcgggaaaca gccggtcgag gcccgcgcgg
     4081 agaagcccct cgtcgaaatc caggggcggc gcagccagac gctcggcgat gcgctcgcgt
     4141 atgatgcgct cgtattcgta caggcggtgc aggtacaacc gtccggcctc atcgagcacc
     4201 agcggccgga actcgcccgg ttttcccacc gcaggagacg agcggagcgc tcgcacccac
     4261 gcggcggcgc gggggagcgc gacggtcgcg gcctcgcgcg gacaatcgtc cacggcgtcg
     4321 ccgagcggaa cgcccgccat cgcattcaga tcgatgcaga tgtcgccgtc ggccaggcgg
     4381 cggctcgcca gaagcgcgcc caaccctacg gcctcgtctg atccgcctgc ggcgcgttca
     4441 acgaacgcgg cgaagtgccg gtcgatgtcg cgccagcagt ccagcgcgcg gatgtcaacg
     4501 gattcgtttc tacgcgacgc catcgccatc acctcgcgtg agatatcgcg tcagcgcctc
     4561 gatgagcgcg cgcggcggcc ggtcggcgaa gacgccgcat ccgggcgcgc cggagggatc
     4621 gaccccgcgc acgtagacgt atcgcacccc gccgaagttt cgatcgtact cgtagccagg
     4681 cagccgcagc gagagatagc ggtgcagcgc caccgcgtac agatggtact gcagcacgta
     4741 ggcgtgacgg accatgtccg ccgcaagcgc gtccggcgtg tacgccgcgg gcgcatcgcc
     4801 gagccagttc gatttccagt caatgataga gtaccgcccc gcgtgctcga ccaccaggtc
     4861 gataaatccg cggacgaatc ctcggatccc ggcgaagccg aggccggccg ccgcgctcga
     4921 gaagtttcgc acaacgggat tcgccgcgtg caccgcgaac gcctcggcta cccccgccgg
     4981 ctcgaccgcg gacaacggga aatagtactc cagttcgctc aggcggcgtt cgcggcccac
     5041 tgtccgaagg gacactccat cgtccccgag gggggccgcg cacacccgcg cgagcattgt
     5101 actacaggcc gcactccagg ccgcgtcgat cccgtgacgg gccagcgcgg cgtcgatcac
     5161 gccctcgaag ccggtctcgt cggtaaaatc gacgcgctcg aacatctcat ggatgcactg
     5221 gcccggtcgc gccccgcggg ggaagcgggc gatatcgtcg aacgcgacgg gcgccgcatc
     5281 gggttcgaaa ggggcgaaat gggcgtcgta atccggacgc tcgccgctct cccggcccga
     5341 tgccgcaagc gccgagtagc tcgtaacggt ccagtgcgcg ccaatcgatc ccgtgaacgg
     5401 acgcggtacg atcccgccgg gcggcgcggt cgctgcgcga agcggcgggg cgttctcgtg
     5461 cggaacgggc cgcacgacga tggcgccgcc ggatcgctcc gccagcgcgg aacggaacga
     5521 tgcgcgctcg ccggcgggaa gcgacttcac gtgcccttcg agcgccccga cgcagtcggc
     5581 gggatcaagt tcatccggcg catacaggag atagctcggc gccgccgttt cggcgccctt
     5641 gatgtttccc caggcgaaat agcagcggtg gatggcgcgg gtgagcgcga catagagcag
     5701 gcgcacgttt tccgacaaca cctcgcgctc ggcgcgccgc cggaccgcat cgtcgcccga
     5761 accgaggtcg accgtgggcg agaagtcctt tggatcgcga tacagatacg gcttccccgg
     5821 tttgatatcg gaggcgcgcc acaggcaggg gcaaaacacg atggggaact ggagcccctt
     5881 gctgccgtgg accgtgagga tcttcaccgc cgcgtcgtcg gtctccaggc gaagctgctg
     5941 cgcatcgtcg gtcccgggcg agacgatccg cgcccccagc caggcgacca cgccctcggg
     6001 gcggaggtgc cgatcgcgct ccgcgccgtg ccccagctcc gcgagatgga tgaggttcgt
     6061 gagcctgcgc tcgccgtcgg gaaacgcgag gagccgcgga cgcacctcga gctcgtccat
     6121 gaaggcccgg atggcgcaat agaacccgtc gcgcatccat cgctcccgcg cgcgggaaaa
     6181 ggcgcgtgca aagcgctccc agcgcgcctc gtcctccgca aggccgaaga ggtccgggcc
     6241 ggagaggccg atcatgtcgg tcgagagcgc cgcgcggaac cggtgctcgg gaccgggcag
     6301 cgcgatggcc atgaggcagc gcatgagctc gagcgcctcg cgggtggaga acacgctctc
     6361 cgccccgtag aggacgcccg ggacgccgaa ggcggtgaga tggtcgcgca taacccgggc
     6421 gtgcgcattg ctgtgcacca ggatggcgat gtcgcccggg cgcaccggac gcgtaccgat
     6481 ccgcgccgca tccggcgcgc cgtcgtcgag caggcggacg atctccgtcg cgaccgcgcg
     6541 gccgagcatc gggagcgccc gtccccgcgg gacggtcccg cgctcctccg gatcgtcgag
     6601 cgaccagaac tcaaacgcgg cgccgggccg cccgccgagg gtcatcgctt cgcggcgcgg
     6661 attctcgccg gcctccgcag gggaaaaccc gatcccgtcg atgacgaagg ggttcgcgac
     6721 ccgcgagaag atcgcgttca ccgcgcggat gagggcggga tcggaccggc ggttcgcgag
     6781 cagcgtgaag gccgcgtcgt ccccgccggc gtcgcgcttg gcgcggatga acgaaaagac
     6841 gtcggccccg cggaaacggt agatcgactg cttcgggtcg ccgatgagaa aaagcgcgct
     6901 tcccggcgcg tcgaaggcgg cgcgaaagat atcgtactgc acctggtcgg tgtcctgata
     6961 ctcgtcgatg agcgccgccc ggaagcgcgc gcggacggcc gccgcgagtc cgccgccgcc
     7021 cgtgacggcc gcgtgcgtgt cggtgatgat gtcgtcgaag gaccgctcgt tgcgtgacgc
     7081 catgcggcga gcgagctcgc gatcggcata ggcgaaaaat gcggcctcgt gacggcgaaa
     7141 gaaccgttcc agcaggccgt ccgccgcttc gcgcagcgcg agcagttcct cgcagcggac
     7201 aaagaattcg tgctcgggag cgtcgtgccc ttgccgcatc gcgccggcga tcacgcgtcg
     7261 ggagaaccgc accagcggat cacactcgcc gcggccgtcg ccggcggcgc aaaacgcgtc
     7321 gagctgcgca taccacgatt ccagatgacg cgcctgatat ttctgccggt tgagccccgg
     7381 cgcgccggca aggagctccc ggacgccgtc gctgtcgcgc tcccatgccg accgcagctc
     7441 cgcgaaggcc gcgtccagct tggacagcgt atcgacgata tcggcgtgat cgggcgcgtc
     7501 cacgcgcggc aggatgtcga acgatcgatc gagcggccga agacgataga gctgtgcgaa
     7561 atgcccgggg ccacggcttc ggcgcgcaag ctcgcgcgcg atcggccccg gaagattgta
     7621 aaaactggtc cgccagtaat cggcggccac cgcagcgagc agatcgctcc ggtcggccac
     7681 aagccccgta tcgaagagcg acccgctctc gaaggcgttc tccgatagga cgagccggca
     7741 gaagccgtga atggtatgga tggccgcctt gtccagatca tggtaggcga cctcgagcgc
     7801 gatacgcgca ccctcgctgt ccgcataccg cgccgcgagg gcggccatga gcgcgtcggc
     7861 gggatcgggc gggaggcgcc gcgcgagcat cgcatgcgca tcgcggagcc gcgcgcggat
     7921 gcgttcccgc agctcctccg cggccgccac cgtgtaggtg accacgagga gcgaatcgac
     7981 ggtcaaggcg cgctccagca gaaggcgcag gtacaatccg gtgatcgcat gcgtctttcc
     8041 ggtcccggcg ctcgcctcga tgacgcgcac gccgtcgagc ggacaggcgg tgatgtccag
     8101 cggcctcacg gtttcgcctc cgactggtgc gccagcatgg gcatgaaaac atgccgggcg
     8161 cattcggcaa aacgcgcgtc gatggggtcg gccccggaga agaggcgcgc gacgtactcg
     8221 tcgtcggcgt cgccgcgcgc gaactcgctt ccgagccacg catcgcgcgc gcgcgcacgg
     8281 gcgtccggct ccgacagctt gccccgggcc gcctcctcgg catataccag cgagctgcgc
     8341 ggaaaaaacg ggagcggtcc gcgcatcccg gcgtcgtaga gatccatgag cccggcaagg
     8401 tagccacccg cgtcgtcgaa ggcgtccatc tcgaatcgcg cgatctcgtc cttcttctcc
     8461 cggagcgaga taaagtgact tacgcccgaa gaggccggat cgaggagcag catgatgcag
     8521 tgcgcaatcc acgcgcggag ccggtcgctc gcgcggcagc gggcatagcg gtaggtcgtc
     8581 aacccgcgcg gccccggcac cggcacggcg ccgacgatcc gatacgcgcc aatcgtcagc
     8641 gaaaacgggc gcggcggcgg atcaccctcc gggagaaacg gcagcagcgc acgatggaac
     8701 gcggacgcac cgcgcgcgag cgcatccacc attcccctgc cgatcccgcc atgcggcaat
     8761 tccccgcgcg cggcgagccc gtccacgata gcgtcgagcg aatcgccccg cagcaccccc
     8821 tcgaggacat gccggtttac ccgccaggcg tcgatcccct cgagcgtgaa gggctcctcg
     8881 tcgcgcagtt cgccggcgat gggcccgtat cgcacgccca gacgcttctc gagcagggcg
     8941 cgcgcgggat tggtaaaaaa accggcaagg gtgtcgagcg cgatctcgcg ctcctcggtc
     9001 gggagcgcaa tgagcggtcc cgggaaaaag gacggcgggg ccgtgcggga aaggagcgcc
     9061 cgtgcggcgg cgctctgcgt ctcggaaaag gtgtagaggg cgccatcatc cctaaaatag
     9121 cgcgggctga acgcctgcag gggatggcgc gtgaccaggg gcttacattc gggatggccc
     9181 tgctcgatgt agtccacgag ctcgctgacg acgatcgagg gcggaatggg acggttgtcc
     9241 tgaacgccct gcccgcaata gctgatgtac agccggtcgc gtgcggagag gatggactcc
     9301 aggaagagat agcggtcctc gtcgcgcagc gagcggtcgc cgggccggaa ctcgtcggcg
     9361 atgcggttga accccgccgg aacgtcgcgc cgcgggaagc tcgcgtcgtt catgccgatc
     9421 atgcacacca cgcggaaggg gacgctgcgc atgggcatga gcgcgcagaa ggtgagccgc
     9481 ccggtgatga actcgccacc gccgcgcgac gcgccgatgg cgtcggccat gaaggcggcg
     9541 agcagccgcg cggacacggt cccgtcgacg tccgacccgg tggcgacctc cgggaggcgc
     9601 gcggcgaaga cgcgcaccgc atccagatcg cgcgcgctct cctcgtccgg gtcgaagaaa
     9661 tcgtcgagcg cgccctccag cacgacgcgc cactctgcga ggggtttcgg gcggcagagc
     9721 gtttcgtgca acgttcgaag cgcttcgaga aacgaaagaa ggcgtccgag ggcgacggcc
     9781 gcatcgccgg cgatcggatc gtacgggagg acgccgccga accgttcatc gccggcggcc
     9841 agcatggcgt acccggcgat gaggcggtcg atcccggcgg cccacgtgtt ccccggagtc
     9901 gccgggaggc cgagggtcgc gcggtgcgcc gcgtcgatgc cccaggtcgt gcgcgttccc
     9961 tcgatccacg cgcggagcag gtcgatatcg ccctcggcaa gcccgcgccg ccggcggatg
    10021 tcgggacagg caagcaggcc catcaccgcg cccgcctcga accggccctc cggcagctcc
    10081 aggagcgcga agagcgcggc aatcgcgggg ctcgtcgcgc ggagcggacg gtccacaatc
    10141 gatatgggga cgtgcggcgc gccgtcgccg ctcccggaga aaaccgcctg cgcgaagggc
    10201 gcgtaggcct cgatgtcggg tgcgaggacc acgatgtccc ccggcgtaag gccgggcagc
    10261 cgctcgaagc aatcgagcac gaaatcgcgc aggacctcga tttcgcgcaa cgcgctatgg
    10321 caggcgttca cgaccacggt gccgtcggcg gcgacgacgg cccgcgccgc gtcatcgtcc
    10381 ccgccgccgg gcgcgtctcc gcgatcgacg accgcgtaga tgtcgtgctg gatgcggtgg
    10441 agcagcgtgt cctctccggg atcgtcgccc gcatagcgat cgtccggatc gccggcgaac
    10501 agcaggccca ggaaatcttg cccctgcctg cccatcgagg ccagcagcgc attgccgcgc
    10561 tcgaggtggc tgtcgcgcgc ggtcgcgctg ccgtccgcgc ccagtcgccg cgcgatggag
    10621 cgcccggggg cgaggtccag ccagtagtgc ggcgagggat tgagctggta cagggtgatc
    10681 tcggccaggg ccgacgccgc ccgcagaacg tcgagatgaa agcgcggaag cgacgagacg
    10741 ccgaacacgc ttatgcggcc cggcaccgcg gcgcgcatag catcgtcggg cgttgcgagc
    10801 cgcgcgaaaa aatcccgctg taggcgcggc ggattgctga agcgcatacc gtcggcaagc
    10861 agccgccaga ggcgtgcctg ccagtcgtcg tcggcgcccc cctcccagcg caggatgagc
    10921 tcgggccggt aggtgagata ctggtcgaag acgtccacga tccgcgccgc aagctggtag
    10981 cgcttgaggc cggccggatc gtcgccgaga taccgcgcaa tcgcggcgaa ggccggatcg
    11041 ggggcgcatt cgggcaggag acgcatcacc cgccacgtca tgatgtcggt gttgaagagc
    11101 cgttcattcg gcgcccccgg aagcgccgct ccgatgagcg cgttgaccat ctcattgggc
    11161 aacagaaaac ggaagttggc ccacaccccc agtctccggg ccagctgcag cgat
//
LOCUS       DAORHF010000111        11141 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5060_length_11141_cov_6.339888, whole genome shotgun sequence.
ACCESSION   DAORHF010000111 DAORHF010000000
VERSION     DAORHF010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5060_length_11141_cov_6.339888"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PKJ16_10355"
     CDS             <1..748
                     /locus_tag="PKJ16_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV47432.1"
                     /translation="ADTTSTLAERFAPLFSGILDGMPITSARLAKLYFNKWNLRADAD
                     SVPASIFELLFLNVLAETVRDEVGEDARDFIDNSRYVTDTFFALLEADQSMLFDDVGT
                     ETEHEYRDSIFDRAFLKALIALNKSRGPLMEEWRWGALFRGEFGLPINTRIGPLTRKI
                     YSFRKTPLAGDSSSLNRAGVTLAGKGIAPTSVTTMVGYAHGGSMAFGTAFGISLNPWS
                     DYYSLTPALRRIEPFAGVSGNRTLRIVPER"
     gene            complement(950..1276)
                     /locus_tag="PKJ16_10360"
     CDS             complement(950..1276)
                     /locus_tag="PKJ16_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47433.1"
                     /translation="MKKIAAVATIVVFACAIVAGFAADNQKLIVGVWKFDMGGGFSGV
                     AEYKADGTFTQKIGKMTISGKYTVKGAALTTDAQGQKTVFTIVSITAKEMVQKRGDGK
                     TMKFTR"
     gene            complement(1340..1978)
                     /locus_tag="PKJ16_10365"
     CDS             complement(1340..1978)
                     /locus_tag="PKJ16_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNV47434.1"
                     /translation="MIRYDIVICGIGGQGVISLGTILKLAAIEEGVSVMGAERRGGAQ
                     REGSVTSNIRYRGLSPGEAHDERAMATSGLIPAAGADMMIAMEPLEALRHAALLNERS
                     VVIVNDFPLIPVSVRIGERAYPARDEIYARLRRFTPHVHVVDIDGLSKQNFNSLRQVN
                     TVSLGLASALGALPVSEESILATVKQRFPDFETNKRAFDLGRSASGAATHGR"
     gene            complement(1975..3891)
                     /locus_tag="PKJ16_10370"
     CDS             complement(1975..3891)
                     /locus_tag="PKJ16_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV47435.1"
                     /translation="MHPLGSREKGTTILAMGNEAIVRGGLEAGIGFFTTYPGTPASEI
                     GVEYMALRSEFPHLYVEFSINEHVAAHAAQGASWANIRAMTAMKHVGMNVAAEVLHFA
                     GYTGVKAGFVVVIGSDPGATSSTSEQDDRWYSLHTHLPILEPATIQEAKDFTALAFEL
                     SERYNLPVIINAPSKLCHNIGSLSLGSLPATFRVKGTFERNPSRYINLFAGSVANHRA
                     GMTSARRLADDAPSLGLNRVLSGAGRTGFISSSINYLYLMEALSILGVSDAPVMKIGM
                     SYPLNAGEIAAFARGLERIYVVEDLEGFLEYQIKRLLYDENLRTEIVGKDIFPDYGEL
                     DVDLVTGILKDELARPLPANVQRTAALAAELTADIPPRQGAYCTGCPHRSTLYSIVKA
                     TERKVIFAGDIGCYTLSCLPPFRAFDWVTCMNCGTGIAHGIMHTNQDEEVIAYVGDST
                     FFHSGLPNLINAVQNGTSMVLVILDNKWVAMTGHQPSPTTARGVRGDTMKPVDLKGLL
                     KSIGVTYVRTVSPHNIKQTIVTLHDALAAKSGVRVIIAEEECALQAERRATFSPPEYE
                     VYYQIEPERCQKCNECYVDFGCPAIRSREAGGDFHYYIEESTCIRCGACHDLCLNSAI
                     LRTEIKRPGREAAS"
     gene            complement(3901..4440)
                     /locus_tag="PKJ16_10375"
     CDS             complement(3901..4440)
                     /locus_tag="PKJ16_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47436.1"
                     /translation="MDTVRYLPTINSAADIDALPNRLKTEEPLLVGEYGSLNPLAFAT
                     AIRNVNSGKKFYELILRNKNRELVESSICTAAAVGFDGVVIASGAFNKSDTMGKPVYD
                     LDQAQALLLAISLRRTGKIPSGFALGVRSAGGNGAVRERNSFYLREGADFILITGSAN
                     TGAAAPTDSADKIGTISEL"
     gene            complement(4443..5087)
                     /locus_tag="PKJ16_10380"
     CDS             complement(4443..5087)
                     /locus_tag="PKJ16_10380"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HNV47437.1"
                     /translation="MIVTRKKDIDSIREHLADARSVVIVGCSECAAVCRTGGSEQVKE
                     MSGLLADRTVCATISIESPCDKRITSRDLKRLTRELEEADAIVALTCGSGAQAIAEVS
                     GKRVIAALDTSFLGMVERLGRFYERCSQCGECILNDTAGICPVTLCPKQVRNGPCDGV
                     RADGTCEVFEDRPCVWRTIHERLSARNHPERFTAYHPPVCWSHHHVPREELQGH"
     gene            complement(5253..5990)
                     /locus_tag="PKJ16_10385"
     CDS             complement(5253..5990)
                     /locus_tag="PKJ16_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008283456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HNV47438.1"
                     /translation="MRKILWVLYQPYKWLIFAPYLAISTLFFGMVAVILSTLINPRIG
                     SLIGGALWARLNAYMTPIWVTVAGRENIDKKQSYVIVSNHQSQYDIFVLYGWLGIDFK
                     WVLKQELRKIPGLGIGCEKVGHIFIDRSNHEKALASLNAAKKKIVNGTSVIFFPEGTR
                     SRDGSLGEFKKGAFKMALDLELPILPVTIIGTKNILPSDTTNLMPGSARLIIHSPIPI
                     TGYTDENIEGLMDAARTTIAEGLPKDY"
     gene            6198..7400
                     /locus_tag="PKJ16_10390"
     CDS             6198..7400
                     /locus_tag="PKJ16_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV47439.1"
                     /translation="MAQQAGGQQEYILKRKKLSGFFFESALPNEYLVQIGQKTVRPVL
                     GGRKLRLFRKFLRVPASVQTLNFVTDNANIDFQGIGIEGYASWRIDPSNPARSITTLD
                     FFDENDPMARTNNELRTICVEAVRHVISNMTIDEAMKKKEDIAERLKAQLKEVEGQWG
                     IVFDQVGIEKVRIMSNKLFEELQAQFRDRLRLDVERTRIATDQEIATEQSTMKEKTEL
                     KAIETDKKIELARTENKARQKEYEIEEARTISLKERSVREEEFRNEIAFKIEQENRTS
                     EFEQLKKNLAITLQDIELKVLATQREIEDIGNAMQRGRLEVEKFKREIGQIFGPDALT
                     SAFIATLPKLFEAITISNYSVIDSGGQGGISPVGKIIAEIAQVLRASGFTWPSAVKPG
                     DDGAGPRE"
     gene            7397..8314
                     /locus_tag="PKJ16_10395"
     CDS             7397..8314
                     /locus_tag="PKJ16_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47440.1"
                     /translation="MKLFDRLFRRTRAAGGRRAIADSRFVRYAESDMSIMCSLGAFTY
                     SSECQNLFLNRFTIDELRRIIAEIGLEGYLASKGFSNLDITVTRNDSYMHMLRIYSGA
                     RDPERLLVELKLSEVHYTLPEPLVKGIIVPEKVSLLAVEWLTLQNPHAAFSPDRPKLP
                     GQDRPGLGAVACMARFLEIIAGEVKTAGIMDIPDHFHNAVMYARLFLFMDPEREGLLR
                     AVLRDLPGLSLYDLSWGFMTESIINEKTGKPEAHVAAEQVYPVSKTLQEYFTSKPYRE
                     RVAAAMESRRLVFNRERMLARQKKHPEFA"
     gene            complement(8315..8869)
                     /locus_tag="PKJ16_10400"
     CDS             complement(8315..8869)
                     /locus_tag="PKJ16_10400"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV47441.1"
                     /translation="MKRNFSLARSCAALPLILLLAGTGCASREVWVSTNAPDPKGIKL
                     ALLDFDIRDKKTGERLPQVGTQFSTALSTGFMKAGFAVIDRKNIGVILKEQEFQQTGI
                     VDPKTAVKFANIMGVRYVVFGSGRANPTGSRSYHFLHEATITMLDAQSGEQVMVATWS
                     GAGVEPVSAARKIGDEIAEKWTKR"
     gene            complement(8956..10221)
                     /locus_tag="PKJ16_10405"
     CDS             complement(8956..10221)
                     /locus_tag="PKJ16_10405"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HNV47442.1"
                     /translation="MTTHGPDVAPGLSRACAIACASYAAAVIAVAALGVMGDSRPESI
                     AAARLHFTHEQIDAGAAFARMGIVSGALYQLALVAGLALAVAHGLDMKLAARIGASIR
                     SGVAATALSWTALYAAMALISLPHALYAGYYRSSIFGLMSTDLGTWLARWCAAKLISI
                     AIAASAATVLTLILAHTKRARIYLPAAVLCIGLALSYALPRVVTPLFYTTAPIAPGAQ
                     RDRITALAAKSGMAVEEIFVIEKSAYSLEANAYFIGAGATGRIYLYDTLLKAFRDNEV
                     ALIVAHELCHFREEHALLGLLLWSIGLALAFPLGDRLARAATGVPLRELAGAHRYAAL
                     TFMVVIASFAARPLENGVSREMERRCDRYAIALTRDPDGFVAMQRRLAAANRSSVLPH
                     PAHAWITRSHPPVLERISLADEHRARDGR"
     gene            10360..10869
                     /gene="def"
                     /locus_tag="PKJ16_10410"
     CDS             10360..10869
                     /gene="def"
                     /locus_tag="PKJ16_10410"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HNV47443.1"
                     /translation="MNLQLCYYGNEVLRHKAAEVAGIDQGVIDLFDSMFNVMQRARGI
                     GLAAPQVGISQRIIVVDIESFKGPALALANPVITATSEELAPYEEGCLSVPGVNGTVV
                     RPAAITVQALFPDGNEARFDAEGLLARVLQHEIDHINGILFVDHLEDHVRKELTPELK
                     KIKKLNKGA"
     gene            10866..>11141
                     /locus_tag="PKJ16_10415"
     CDS             10866..>11141
                     /locus_tag="PKJ16_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNV47444.1"
                     /translation="MRIGFFGTPEIAAFCLARLMERHEVAFVVTGCDKPRGRCLQVSG
                     CPAKCAALERGVPVLEPEHLRDPALPDELARFGAELFVVVAYGAIIPR"
BASE COUNT         2019 a         3378 c         3664 g         2080 t
ORIGIN      
        1 cgccgacacc acctcgacgc tcgccgagcg cttcgcgccc ctgttctcgg ggatcctgga
       61 cgggatgccc atcacctcgg cgcgccttgc gaagctgtat ttcaacaagt ggaacctccg
      121 tgcggacgcc gattcggtcc ccgcgtcgat ctttgagctt ttgtttctga acgtgcttgc
      181 ggagacggtc cgcgacgagg ttggggagga cgcgcgcgat ttcatcgaca acagtcgata
      241 cgtgaccgac accttcttcg cccttctcga ggccgaccag tcgatgctgt tcgacgacgt
      301 cggcaccgag accgagcacg agtatcgcga ctccatcttc gaccgcgcgt tcctgaaggc
      361 gctgattgcg ctcaacaagt cccgcggacc tctcatggag gaatggcgct ggggcgcgtt
      421 attccgcggg gagttcggcc tcccgatcaa cacgcggatc gggccgctta cgcgaaaaat
      481 ctactcgttc cgtaaaacgc cgcttgcggg cgattcctcc tcgctcaatc gcgccggcgt
      541 gacacttgcg ggaaagggca tcgcgccgac atcggtgacg acgatggtcg gttatgcgca
      601 cggaggcagc atggccttcg gaacggcgtt cggcatctcc ctgaacccat ggtcggacta
      661 ttactcgctc actcctgcgc tccgacggat cgagccgttt gcgggcgttt cgggcaaccg
      721 gacgctgcgg atcgttccgg agcgatagcg gcatgcggcc caatgccgta cgtgccggaa
      781 tttaatgcgc accgggctcg cctccgtcaa gttgcaatta tggtcctgcg aggcgactgc
      841 accacgcgaa gattgatcgt ggccgtccca atgctctcga atcagatgga gaataaaaaa
      901 ccggccttat gtaagaggcc ggttttttcg gtgcagcctt cgggaatggt caccgggtaa
      961 atttcattgt cttgccatca ccgcgctttt ggaccatctc cttcgcggtg atagacacga
     1021 tggtaaagac ggtcttctgt ccctgcgcat cggtcgtgag ggcggctccc ttgacggtat
     1081 atttcccgga aatcgtcatc ttgcctatct tctgagtaaa cgtaccgtcc gccttgtact
     1141 cggcaacccc gctgaaaccg ccacccatgt cgaatttcca gacgcctaca atgagcttct
     1201 gattgtcggc ggcaaatccg gcgacgatgg cgcatgcgaa cacgacgatg gttgcgactg
     1261 ctgcgatttt tttcattgat cctccttgat tcattaaact agattattgg tactatacgg
     1321 cgatgacgca attcgatcgc taccgaccgt gtgtcgccgc gcccgatgcc gatctcccca
     1381 ggtcgaaggc gcgcttgttg gtctcgaaat cgggaaagcg ctgcttcacg gtcgcaagga
     1441 tcgactcctc cgatacggga agcgccccca gcgccgatgc caggccgagc gatacggtgt
     1501 tcacctgccg cagcgagttg aagttctgct tggagagccc gtcgatgtcc acgacatgca
     1561 cgtgcggcgt gaagcggcgc aggcgcgcgt agatctcgtc gcgcgcggga taggcgcgct
     1621 cgccgatgcg caccgagacg gggatcagcg gaaagtcgtt cacgatgacc accgagcgct
     1681 cgttgagcag cgcggcgtgg cggagcgcct ccagcggctc catggcgatc atcatgtccg
     1741 cgcctgcggc cgggatgagg ccggaggtgg ccatggcgcg ctcatcatgc gcctcgccgg
     1801 gcgacaggcc gcgatagcga atgttcgagg tgaccgatcc ctcgcgctgc gcgccgccgc
     1861 ggcgttcggc gcccatcacc gaaacgccct cctcgatggc cgcgagcttg aggatggttc
     1921 ccagcgatat caccccctgg cccccgatcc cgcagatgac gatgtcgtag cgaatcacga
     1981 cgccgcctcg cgcccggggc gcttgatctc cgtgcgaagg atggcggagt tgaggcacag
     2041 gtcgtggcag gcgccgcagc gaatgcaggt cgattcctcg atgtagtagt ggaaatcgcc
     2101 gcccgcctcg cgcgaacgga tggcggggca gccgaaatcg acatagcact cgttgcactt
     2161 ctggcagcgc tccggctcaa tctgataata gacctcgtat tcgggcggcg agaacgtcgc
     2221 gcggcgttcc gcctggaggg cgcattcctc ctcggcgatg atcacgcgca ccccgctctt
     2281 cgcggcgagc gcgtcgtgca acgtgacgat ggtctgcttg atgttgtgcg ggctcacggt
     2341 gcggacatag gtcacgccga tggacttgag gagccccttg aggtccaccg gcttcatcgt
     2401 gtcgccgcgc acgccgcgcg ccgtggtcgg ggagggctgg tggccggtca tggcgaccca
     2461 cttgttgtcc aggatcacca ggaccatgct ggtcccgttt tgcacggcgt tgatgaggtt
     2521 gggcagcccc gagtgaaaga aggtggagtc gcccacgtag gcgatcacct cctcgtcctg
     2581 gttcgtgtgc atgatgccgt gcgcgatgcc ggtgccgcag ttcatgcagg tgacccagtc
     2641 gaaggcgcgg aagggcggca ggcacgagag cgtgtagcag ccgatgtccc cggcgaagat
     2701 aaccttccgc tcggtggcct tcacgatcga gtagagcgtg gagcggtgcg ggcagccggt
     2761 gcagtaggcg ccctgccgcg gggggatgtc ggccgtgagc tcggcggcga gggcggcggt
     2821 ccgctgcacg ttggccggga gcgggcgcgc gagttcgtcc ttcaggatgc cggtgaccag
     2881 atcgacgtcg agctcgccgt agtcggggaa gatgtccttt ccgacgatct cggtgcggag
     2941 gttctcgtcg tacaggaggc gcttgatctg gtactcgagg aagccctcga ggtcctcgac
     3001 cacgtagatg cgctcgagcc cgcgcgcaaa ggccgcgatc tccccggcgt ttaacggata
     3061 gctcatgccg atcttcatca ccggcgcgtc gctgactcca aggatggaga gcgcctccat
     3121 gagatagaga taattgatgc tggatgagat gaagccggtc cttcccgcgc ccgacagcac
     3181 gcgattcagt cccagcgacg gggcgtcatc ggccaatcgc cgtgcgctgg tcattcccgc
     3241 acgatgattg gccaccgacc ccgcgaaaag attgatatag cgcgacgggt tgcgctcgaa
     3301 ggtccccttc acgcggaacg tcgcgggaag cgatcccagg gaaagcgagc cgatgttatg
     3361 gcagagcttc gacggcgcgt tgatgatgac gggaagattg taccgctcgc tgagctcgaa
     3421 ggcgagcgcg gtgaagtcct tggcctcctg gatggtggcc ggctccagga tgggcaggtg
     3481 cgtatggagc gagtaccagc ggtcgtcctg ctcgctggtg gagctcgtcg cgccggggtc
     3541 ggagccgatc acgacgacga agccggcctt cacgccggtg tagcccgcga agtgcagcac
     3601 ctcggccgcg acattcatgc ccacgtgctt catcgcggtc atggcgcgga tgttcgccca
     3661 gctcgcgccc tgcgcggcgt gcgccgcgac gtgctcgttg atgctgaact ccacgtagag
     3721 gtgcggaaat tcggagcgaa gcgccatgta ctccacgccg atctccgagg cgggcgtgcc
     3781 ggggtaggtg gtgaagaagc cgatgcccgc ctccagaccg ccgcgcacga tggcctcgtt
     3841 gcccattgcc aggatcgtcg ttcccttctc acgggagcca agcgggtgca tcctgtgctc
     3901 ctatagctcg ctgattgtgc cgatcttgtc ggcggaatcg gttggggcgg cggcgccggt
     3961 gttcgccgaa ccggtgatca gaatgaaatc ggcgccctcg cggagataga aggaattgcg
     4021 ttcacggacg gcgccgtttc ctccggccga tctgactccc agcgcgaaac cggacgggat
     4081 cttcccggtg cgtcgaagcg agatcgcaag caggagcgcc tgcgcctgat cgagatcgta
     4141 cacgggcttg cccatcgtgt cgcttttatt gaatgcgccg gaggcgatca ccacgccgtc
     4201 gaatcccacc gccgccgcgg tgcagatgct ggattcgacg agctcccggt ttttgtttct
     4261 caggatgagt tcgtagaatt tttttccgct attgacattc cggatggccg tcgcgaaggc
     4321 gagcggattg agcgaaccgt attcgcccac caggagcggc tcctccgtct tgagacggtt
     4381 cggcagggcg tcgatgtcgg cggcgctgtt gatggtcggg aggtagcgga cggtatccat
     4441 gatcaatgcc cctgcagctc ttcgcgcggc acatgatggt gcgaccagca gacgggcgga
     4501 tgatacgcgg tgaagcgctc cggatgattg cgcgcggaga gccgttcgtg gatggtgcgc
     4561 catacgcagg gccggtcctc gaacacctcg caggttccgt cggcgcgcac gccgtcgcag
     4621 gggccgttgc gcacctgctt ggggcacagc gtgaccgggc agatcccggc ggtgtcgttg
     4681 agaatgcact cgccgcactg cgagcagcgc tcgtagaacc gccccagccg ctccaccatg
     4741 cccagaaacg acgtgtcgag cgcggcgatc acgcgtttcc cgctcacctc ggcgatggcc
     4801 tgtgcgccgc tcccgcaggt gagcgcgacg atggcgtcgg cctcctcgag ttcgcgcgtg
     4861 aggcgcttca agtcgcgcga cgtgatccgc ttgtcgcagg gcgattcgat ggagatggtc
     4921 gcgcagacgg tcctgtcggc aagcagcccg gacatctcct tcacctgctc cgatccgccg
     4981 gtgcggcaca ccgccgcgca ctccgagcag ccgacgatga cgacgctgcg tgcgtccgca
     5041 aggtgctcgc ggatgctgtc gatgtctttc ttgcgggtaa ctatcatgcc gtgcgccccc
     5101 ggtgaagttc gacgccaact atagcgcggg ccgggagctt ggcaatagtt tttctggtcg
     5161 gaggggggcg atgagatcac ggctctggtg cgagccatgt attatttcat gcaggatttc
     5221 ctcggtcgag catgaacggt tatcgaagga aatcagtaat ccttcggaag cccttcggcg
     5281 atcgtcgtac gcgcggcgtc catcagtccc tcgatgttct catcggtgta tccggtgatc
     5341 gggatggggg aatggatgat cagccgcgcc gaaccaggca tgagattcgt cgtgtccgag
     5401 ggaaggatgt tcttggtgcc gatgatggtc accggcagga tcggcagctc gaggtccagc
     5461 gccatcttga aggcgccctt cttgaactcg cccaggctgc cgtcgcggct gcgcgtcccc
     5521 tcgggaaaga atatcaccga ggtgccgttc acgatcttct tcttcgcggc gttgagcgag
     5581 gcgagcgcct tctcatggtt cgagcggtcg atgaagatgt ggcccacctt ttcgcagccg
     5641 atgcccaggc cggggatctt acgcagctcc tgcttgagca cccacttgaa atcgatgccc
     5701 agccagccgt agagcacgaa gatgtcgtac tggctctgat ggttcgatac gatcacgtat
     5761 gattgtttct tgtcgatgtt ctcccgcccc gcgaccgtga cccagatggg cgtcatgtac
     5821 gcgttcagtc gcgcccacag cgccccgccg atgaggctcc cgatgcgcgg attgatgagc
     5881 gtggacagga tgaccgccac catgccgaaa aacagggtcg agatcgccag gtagggggcg
     5941 aagatgagcc atttataggg ttgatacagg acccacagga tttttctcat gacgctttcc
     6001 ccgatcatcg ttcgcgcggc tgaaccgagt ttgttcgcag gcatcattaa ctggcaatat
     6061 ccgcttggca acccaaaagt ataaaaaaag tacacggatt cgtatagggt tgatgatgcc
     6121 gcgcccggga aaaatcaact tgccattatc gcgcgcttcg ggatatgatg gggtcactgc
     6181 agcggaggag ggcggcgatg gcacaacagg cgggcggtca gcaggagtat atcctcaagc
     6241 ggaagaagct ctccggcttc tttttcgagt cggccctgcc gaacgagtac ctggtccaga
     6301 tcggccagaa gacggtccgg cccgtcctgg ggggccggaa gctccgtctc ttccggaagt
     6361 tccttcgcgt gccggcctcg gtgcagacgc tcaacttcgt cacggacaat gcgaacatcg
     6421 actttcaggg gatcggcatc gagggatacg cctcgtggcg catcgatccg tcaaatccgg
     6481 cgcgctccat caccacgctc gactttttcg acgagaacga tcccatggcg cgcactaaca
     6541 acgagctgcg gaccatctgc gtcgaggcgg tgcggcatgt catatcgaat atgaccatcg
     6601 acgaggccat gaagaagaag gaggacatcg ccgagcggct gaaggcccag ctcaaggagg
     6661 tggagggaca gtggggcatc gtgttcgatc aggtggggat cgaaaaggtg cgcatcatgt
     6721 ccaacaagct tttcgaggag ctgcaggcgc agtttcgcga ccgcctgcgc cttgacgtgg
     6781 agcgcacgcg catcgccacc gaccaggaga tcgccaccga gcagagcacg atgaaagaaa
     6841 agaccgagct caaggccatc gagaccgaca agaagatcga gctcgcgcgc accgagaaca
     6901 aggcgcgcca gaaggagtac gagatcgagg aggcgcgcac catctcgctc aaggagcgct
     6961 cggtacggga ggaggagttc cgcaacgaga tcgccttcaa gatcgaacag gagaaccgca
     7021 ccagcgagtt cgagcagctt aagaagaacc tggccatcac cctgcaggat atcgagctga
     7081 aggtcctcgc gacgcagcgg gagatcgagg acatcgggaa cgcgatgcag cgcggccgtc
     7141 tcgaggtcga gaagttcaaa cgcgagatcg gccagatctt cggtcccgac gcgctcacga
     7201 gcgccttcat cgccacgctg ccgaagctct tcgaggcgat aacgatcagc aactactccg
     7261 tgatcgactc gggaggtcag ggcgggatct ccccggtggg gaagatcatc gcggagatcg
     7321 cgcaggtctt gcgggccagc ggcttcacgt ggccgtccgc tgtaaagccg ggcgatgatg
     7381 gagcagggcc gcgcgaatga aattgttcga ccggctgttc cggcgcaccc gcgccgctgg
     7441 agggcgacgc gcgatcgccg actcgcgctt tgtccgctac gccgagagcg acatgagcat
     7501 catgtgcagc ctgggcgcct tcacctacag cagcgagtgc cagaacctgt ttctcaaccg
     7561 attcacgatc gacgagctgc ggcgcatcat cgcggagatc gggctcgagg gctaccttgc
     7621 ctccaagggc ttttccaatc ttgatatcac cgtaaccaga aacgacagct atatgcacat
     7681 gctgcgcatc tattccggcg cgcgcgatcc tgagcggctg ctggtcgagc tcaagctttc
     7741 cgaggtgcac tatacccttc cggagccgtt ggtgaagggc atcatcgtcc cggagaaggt
     7801 ctcgctcttg gccgtggaat ggctcacgct gcagaatcca cacgcggcat tctccccgga
     7861 tcggcccaag ctcccgggac aggaccgacc gggtttgggc gccgtcgcct gcatggcgcg
     7921 ctttctcgag atcatcgccg gcgaggtgaa gaccgcgggc atcatggaca tccccgatca
     7981 tttccataac gccgtgatgt acgcgcggct gtttttattc atggacccgg agcgcgaggg
     8041 gctgctgcgc gcggtgctgc gcgatctgcc ggggctttcg ctctacgatc tgagctgggg
     8101 attcatgacg gagtcgatca tcaacgagaa aacgggaaaa cctgaagcgc atgtcgcggc
     8161 cgagcaggta tatcccgtgt caaaaacgct gcaggaatat tttacatcga agccctatcg
     8221 cgagcgcgtc gcggcggcca tggaatcgcg ccggcttgtc ttcaatcgcg agcggatgct
     8281 cgcgcgtcag aagaaacacc cggagttcgc ctagtcaccg cttcgtccac ttctcggcaa
     8341 tctcgtcgcc gatcttccgc gccgcgctca ccggttcgac gcccgcgccc gaccaggtgg
     8401 cgaccatgac ctgctcgccg gattgcgcgt ccagcatcgt gatggtcgcc tcgtggagga
     8461 agtggtagct gcggctgccg gtcgggttcg cccggccgct gccgaaaacg acgtagcgca
     8521 ctcccatgat gttcgcgaac ttcacggcgg ttttggggtc cacgatgccc gtctgctgga
     8581 actcctgttc cttgagaatg acgccgatgt tcttccggtc gatgacggcg aagcccgcct
     8641 tcatgaaccc ggtcgagagc gccgtcgaaa actgcgtgcc gacctgcggc agccgctcgc
     8701 cggttttctt gtcgcgaata tcgaagtcga gcaatgcaag tttgattccc ttcgggtcgg
     8761 gcgcgtttgt gctcacccac acctcgcggg aggcgcagcc ggtcccggcg agcagcagga
     8821 taagcggcag cgccgcacac gatcttgcaa gactgaagtt tcgtttcatg gtcgagatcc
     8881 tcgtttggcg gaacgatgta tttcgcttca ctgtgccatg cgggaggaac gctgcgcaag
     8941 caaaaaatag tccggctacc gcccgtcccg cgcgcgatgc tcgtcggcaa gggaaatgcg
     9001 ctcgaggacc ggcgggtgcg agcgggtgat ccaggcatgg gccgggtgcg ggagcacgga
     9061 ggagcggttg gccgcggcca gccgccgctg catggcgaca aatccgtccg ggtcgcgggt
     9121 gagggctatc gcataacggt cgcagcgtcg ctccatctcg cgcgagacgc cgttttcgag
     9181 cggccgcgcg gcgaaggagg cgatgacgac catgaacgtg agcgccgcgt agcggtgcgc
     9241 gcccgccagc tcgcggaggg ggacgccggt cgcggcgcgg gcgaggcggt cgcccagcgg
     9301 gaaggcgagg gccagcccga tcgaccagag cagcaggccg aggagcgcgt gctcctcgcg
     9361 aaagtggcac agctcgtgcg cgacgatcag cgccacctcg ttgtcgcgaa aggccttgag
     9421 cagcgtgtcg tagaggtaga tgcggcccgt cgcccccgcg ccaatgaagt aggcgttcgc
     9481 ctcgaggctg taggcgctct tctcgatcac gaaaatctcc tcgacggcca tcccgctttt
     9541 cgcggcaagc gcggtgatcc ggtccctctg cgctcctggc gcgatgggcg cggtcgtgta
     9601 gaagagcggc gtgacgacgc gcggcagcgc atacgagagg gccaggccga tgcacagtac
     9661 ggccgcggga agatagatcc gcgcccgttt cgtgtgtgcg agtatgagcg tgagcaccgt
     9721 ggcggcgctt gcggcgatgg cgatggagat gagtttcgca gcgcaccagc gcgcaagcca
     9781 ggtgccgagg tcggtgctca tgaggccgaa gatgctgctg cgatagtagc ccgcgtacag
     9841 cgcatgggga agcgaaatga gggccatggc cgcgtagagt gcggtccatg aaagcgcggt
     9901 cgccgcgacg ccgcttcgaa tggatgcgcc gatccgcgcg gcgagtttca tgtcgaggcc
     9961 gtgcgcgact gcgagggcaa gcccggcgac gagcgcgagc tgatacagcg cgccggagac
    10021 gatgcccatg cgcgcgaagg ccgctcccgc gtcgatctgc tcgtgcgtga aatgaaggcg
    10081 cgccgccgcg attgattcgg gtcgggaatc gcccataacg ccgagagcgg cgacggcgat
    10141 cacggccgct gcgtagctcg cgcaggcgat cgcacaagcg cgggacaatc ccggcgcgac
    10201 gtcgggtccg tgtgtcgtca tatcgtttcc tcttcgggca tggaatgacg caggctgtcc
    10261 ggcgcatggc gctatcacaa caattttttg attatttccg attaatgcga ttgattttta
    10321 ccggtggcgg cgtgataccc gcccacaacg ggcaacgtga tgaaccttca attgtgttac
    10381 tacggaaacg aggtgctgcg acacaaggcg gccgaggtcg ccgggatcga tcagggcgtg
    10441 attgacttgt tcgattcgat gttcaatgtg atgcagcgcg cgcgcgggat cgggctcgcg
    10501 gccccgcagg tcggcatatc gcagcgcatc atcgtggtgg acatcgagtc gttcaagggg
    10561 ccggcgctcg cgctggccaa tccggtgatc accgcgacat ccgaggagct ggcgccctac
    10621 gaggagggct gcctctcggt ccccggcgtg aacgggaccg tggtgcgccc cgcggcgatc
    10681 acggtgcagg cgctgtttcc ggacggcaac gaggcgcgct tcgatgccga gggattgctc
    10741 gcgcgcgtcc tgcagcacga gatcgaccac atcaacggaa ttctgttcgt ggaccatctt
    10801 gaggaccatg tccgaaagga gctcaccccc gagctcaaga agataaagaa gttgaacaag
    10861 ggcgcatgag gatcggcttc ttcgggaccc cggagatagc ggcgttttgc ctggcgcggc
    10921 tcatggagcg ccacgaggtg gcttttgttg tgaccggctg cgacaagccg cgcgggcgtt
    10981 gcctgcaggt ctcgggctgc ccggcgaaat gcgccgcgct cgagcgcggg gttccggtgc
    11041 tcgagccgga gcatctgcgc gatcccgcgc tgcccgacga gcttgcgcgc ttcggggccg
    11101 agctgttcgt cgtggtggcc tatggcgcca tcatcccccg c
//
LOCUS       DAORHF010000112        11065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5125_length_11065_cov_5.248229, whole genome shotgun sequence.
ACCESSION   DAORHF010000112 DAORHF010000000
VERSION     DAORHF010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5125_length_11065_cov_5.248229"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1364
                     /locus_tag="PKJ16_10420"
     CDS             132..1364
                     /locus_tag="PKJ16_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNV47445.1"
                     /translation="MSHASQNAATIPRWLPLVGGVFSSTTCGALLYAWSVFIKPLNAD
                     FGWSRADIALAFAICCLVFGLMTYPAGRLSDKYGARIVVFIGGAILCAGFVLSGFIES
                     KTQLYITYGLVAGFGGGLVYLPPIATAPKWWPDHRALATGFAVVGLGLGSFIMAPLAT
                     YIIQNYGWRSVFIYVGAAMGVMAILASFTLKTPPAGWKPAGFNPVVPTSGGRETRDFT
                     YQETFKTAQFWMLYVAYFCGSFAGLMVIGHIAGHGIDRGIEPMKAAGAASWLAIFNAA
                     ARVLIGIIGDKIGVKKSFVGIFALQVAAMALLYPAGYTYWALWMVAALIGWNYGSMFT
                     LFPATCIQFFGITSQGTNYGLLFTSWGLAGFAGPYVGGWLKDSTGTYLAPFVVGAVVV
                     AISVIIIALVKAPEKKHA"
     gene            1691..1813
                     /locus_tag="PKJ16_10425"
     CDS             1691..1813
                     /locus_tag="PKJ16_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47446.1"
                     /translation="MGAIEQKGHNAPTVDQALCTRCGLCTKGCPYQAFIAMKDH"
     gene            complement(1822..2742)
                     /locus_tag="PKJ16_10430"
     CDS             complement(1822..2742)
                     /locus_tag="PKJ16_10430"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47447.1"
                     /translation="MGDRAPLRRVRLTAALACAALISGIATLPTRSLFAIDVDTLDPS
                     NRFLFRQIEKPPDDPQAYERIFYKNELIHCTGSNEAAAANNRAIALMEQANYADAEKL
                     LREALARAPLFFPFEYNLGMCLIHLDRLREALVHLRRAANLVPEFSKTYLQIGYVYQR
                     MAKHEIAIDHFRKALAINAKEIDGYVQIGDIYFSRKQYSMAKKYYEGALKVDTRFPNG
                     LLGLAKIHFIKEKYRRAIILFQTIDTTKNYDKSLHYYYAECLYKMQEYEEAYKQYERL
                     LTFKTDKFFLTNSVALIRHKLELTKRFIDR"
     gene            complement(2742..3689)
                     /locus_tag="PKJ16_10435"
     CDS             complement(2742..3689)
                     /locus_tag="PKJ16_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV47448.1"
                     /translation="MVRVAGLTKHFSARKSGFFERPGVVRAVDDIGFTIEAGKTLGMV
                     GESGSGKTTAARSMLRLIEPDAGEVVINGRDVLRLPPAELRSFRRNMQIVFQDPYGSL
                     NPRMTAGKIITEPLRIHRSMEKSELASRLRELLELVGLSPEHADRYPHEFSGGQRQRI
                     GIARAIALNPAFIILDEPVSALDVSIQGQILNLLRDLQEKLGLTFLFVAHDLAVVEHM
                     SDRIAVMYLGKIVEESARTELYREPLHPYTASLLTSIPTAEPSRKGFAVLPGEIPSPE
                     DPPAGCHFHPRCPKVMDTCRSVPPPMKEVGGRRVACHLY"
     gene            3839..4261
                     /locus_tag="PKJ16_10440"
     CDS             3839..4261
                     /locus_tag="PKJ16_10440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47449.1"
                     /translation="MALSKQKTFSLTYQVASLHGTRKDLIAFLMDAALPVDVVSQIEL
                     AAYEALVNIIEHNPHPRAGAKISMRCTIAPARITLTIANTGVHFDPTTAPMPDLAEHF
                     KKGRRRGLGIYIIRTLMDTMEYSFAKGVNRLRMVKRIA"
     gene            complement(4258..5457)
                     /locus_tag="PKJ16_10445"
     CDS             complement(4258..5457)
                     /locus_tag="PKJ16_10445"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV47450.1"
                     /translation="MFINFFYNLKAHGVPVSLHEWITLHHALVANLNDCSLTKFYHIA
                     RAILVKNEIHFDRYDLAFLDTFKDIETTDEMLDKILEGLKKVKELHLSEEEKRQMEQL
                     DLDQVLKNFEEQLKQGHYKGHVGGNKAIGTGGRSTQGAWGYNPAGIRIGQGESRHKRA
                     VQIAEKRTFRNYSSNITLDTRQMKVALSHLRSLLPIGPEERLNLGETIDATCKNAGEL
                     EFVWETKEKKAAKVILLMDAGGSMTPYSQLVELLFSAASGQISRFKHFYFHNCVYQDL
                     WRDIERGDATISTADLLKSEDPDYKLILVGDAEMAPSELTWRNGAIDYWYHNDTPGIV
                     WLQRLRDKFRDSIWLNPLPRRSWDYVQTIRMVRDIFPMFELTLEGLDEGVKFLMQGKS
                     RIVGRAS"
     gene            complement(5447..6301)
                     /locus_tag="PKJ16_10450"
     CDS             complement(5447..6301)
                     /locus_tag="PKJ16_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV47451.1"
                     /translation="MDFFKGSDDYVLSPELRDNVNVSIVLGRPLLVKGEPGTGKTLLA
                     HSIAKGIGKKLIIWNVKSTTKAKEGLYVYDTVQRLNDARFGDRDVSDIKQYIKLGKLG
                     EAFAAEEQVVLLIDEIDKAEIEFPNDLLNEIDEMSFYIPETGVTVKAKHRPICIITSN
                     SEKELPDPFLRRCVFHYIEFPGQELMTDIVNVHFPDLDKKLLKECLKKFYWIREFDML
                     RKKPSTSELIDWIQALLAGGISPKKVESELPFLGALIKKKEDMDVIAGSDKSHYAYSS
                     SARSRGVY"
     gene            6721..7311
                     /locus_tag="PKJ16_10455"
     CDS             6721..7311
                     /locus_tag="PKJ16_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47452.1"
                     /translation="MKVYLKLFIAGMVLLLSSCSYVRIVDILDESSAIVFYTEEGVQR
                     KKNAETVPLEVVRYEKGQKIPILRVKKWVATWTGMSVMKAEEFRAGTKDDSYSRTLIT
                     SVQNQGDGIAKNLMVIETIPARFVVESIGFVDGSGLYGYSLGVKDFNNYGIISKRSGP
                     NGTTIYEIKILKPLNPDQVFSIGFMGHYKVAARKSM"
     gene            complement(8306..9193)
                     /locus_tag="PKJ16_10460"
     CDS             complement(8306..9193)
                     /locus_tag="PKJ16_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV47453.1"
                     /translation="MKRVVIAFCIIALLVPVIAFAGGSSSKCSTKYPIVLSHGMGARA
                     NNLGIGYWYNIPGELRDEGAQVFISDQQAYDRTADRAAELKKYVLYVLAVTGKSKVNI
                     IGHSHGTLDSRYMIANLGMKDKVASYTSIAGPHRGSAVADVLIGVLPDWGEWLVTGIN
                     NTIAMWLLGDTNPDMKGGAYDLRMSYMNGVFNPNTPNQAGIYYQSWAAKIKLVTADFL
                     LTGATYLLLKYYEGDNDCLVSVNSAKWGNFRGIESGAWWCGGVSHFNIVDQFLGITPG
                     FDAPAFFVNIVGELKSKGY"
     gene            complement(9565..10125)
                     /locus_tag="PKJ16_10465"
     CDS             complement(9565..10125)
                     /locus_tag="PKJ16_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47454.1"
                     /translation="MIPFLAEITVIADVGARETIDGIDAGWHRASFSGKTGYIFGGFL
                     TYERPKRSVPEHVPIIQAYFKSARNGYCHHRPEITLGMPESTVIAILGAPRISDAWMG
                     ANAISHSRFTLIIVLPHNNPAQPVSGFMYDLKTERIILEELVIALGKPKEISFNGHDQ
                     CWDILYGRIRFSAEELNGFITGMIVH"
     gene            complement(10352..>11065)
                     /locus_tag="PKJ16_10470"
     CDS             complement(10352..>11065)
                     /locus_tag="PKJ16_10470"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HNV47455.1"
                     /translation="AGELVNPLERPVAEYPSVAVPDRFIVDDSMIVPPSPTPVDIFRG
                     PNIGEPPYTEPLLDSIAGVVGARLGDKITTDHIMPAGARLKYRSNIPKYAQFVFEGVD
                     PAFAAAALANKQKGVYTAVVAGQSYGQGSSREHAAICPMHLGVRLVIAKSFERIHSAN
                     LVNFGIVPLVFANPTDYDAIVKGDAFTVSNLREAVKSMKTVTVKIGGKNCELEIVASE
                     RQREILLAGGLLNYTKQAR"
BASE COUNT         2284 a         3114 c         3244 g         2423 t
ORIGIN      
        1 ctaattggta tgctgcccga ccgtgctaaa caccgcgtca ttacagaata tccagtgcat
       61 acagtatcag taccgtcgtc aacggaatgg gaatggtatg cactcgaaat agagactatg
      121 ggggtaacat catgtcacac gcttcgcaaa acgcagcgac aataccgcgc tggctgccgc
      181 tggtgggcgg ggtcttcagc agcaccacct gcggggcgct cctgtacgca tggagcgttt
      241 tcatcaagcc gctcaacgcc gacttcggat ggagccgcgc cgacatcgcg ctggccttcg
      301 ccatctgctg cctggtcttc ggcctcatga cctatccggc cgggcgcttg agcgacaagt
      361 acggcgcgcg catcgtcgtc ttcatcggcg gcgcgatcct ctgcgccggg ttcgtgctgt
      421 ccggtttcat cgaatcgaaa acgcagctct atatcaccta cggtctcgtc gccggtttcg
      481 gcggcggcct ggtctatctg ccgcccatcg cgacggcgcc caagtggtgg cccgatcacc
      541 gcgcgctcgc gaccgggttc gccgtggtcg gcctgggcct gggatcgttc atcatggcgc
      601 cgctggcgac ctacatcatc cagaattacg gctggcgctc ggtgttcatc tatgtgggcg
      661 ccgcaatggg ggtcatggcg atcctcgcct cgttcacgct gaaaacgccg ccggccggct
      721 ggaagccggc gggattcaat cccgttgtcc ccacgtcggg aggaagggag acgagagatt
      781 tcacctatca ggagaccttc aagaccgcac agttctggat gctctatgtc gcctacttct
      841 gcggctcctt cgcggggctc atggtgatcg gtcatatcgc cggtcatggg atcgaccggg
      901 gcatcgaacc gatgaaggcg gccggcgcgg cgagctggct tgcaatcttc aatgcggcgg
      961 cgcgcgtcct catcggcatc atcggcgaca agatcggcgt caagaagagc ttcgtgggca
     1021 tcttcgccct gcaggttgcg gccatggcgc tcctgtatcc cgccgggtac acctactggg
     1081 cgctctggat ggtcgcggcg ctcatcggct ggaactacgg ctccatgttt acgctcttcc
     1141 cagccacctg catccagttt ttcgggatca cctcgcaggg gaccaactac gggctgctgt
     1201 tcacctcatg ggggctggcg gggttcgcgg gaccgtatgt gggcggctgg ctcaaggact
     1261 caaccgggac ctatctggcc ccgtttgtgg tgggtgcggt cgtggtcgcg atatcggtga
     1321 tcatcatcgc acttgtgaag gcgccggaga agaagcacgc ctagcgccgc gcgttcccca
     1381 attaccaatc gcattccatc aacaatgtac ggcgggtgat tacacacccg ccgtacgcgt
     1441 atgtagagcc aaactctgta ggctcatggc gaaagaaaaa taaaagaggc ggaagagtat
     1501 cacagtcgcc ccgtcgcacg ggattatttt ttttagttga cgaaatgatt atattttagt
     1561 atattctaaa atattaatta tacatactgg tgaaactatg acatcaataa cggccgaaaa
     1621 accgatcatt atcgtcgata gccatcgctg cccgcagaac catccctgcc cgatggtgaa
     1681 gcgatgtccg atgggcgcca tagagcagaa gggacataat gcaccgacgg tggatcaagc
     1741 gctatgtacg cgctgcggtc tttgcacgaa gggctgtccg tatcaggcgt tcattgccat
     1801 gaaggaccac tgaggccggc gttagcggtc gatgaaacgc ttggtaagct ccagcttgtg
     1861 ccgaatgagc gccacggagt tggtgagaaa aaacttatcg gtcttgaagg tgagtagtct
     1921 ctcgtactgc ttgtaggcct cctcgtattc ctgcatcttg tacaggcact ccgcgtagta
     1981 gtagtgcagc gacttgtcgt agttcttcgt ggtgtcgatg gtctgaaaga ggatgatggc
     2041 ccgccggtat ttctccttga taaaatggat cttcgcgagg cccagaaggc cgttgggaaa
     2101 gcgcgtatcg accttgagcg cgccctcgta atactttttc gccatcgaat actgtttgcg
     2161 tgaaaagtag atatcgccga tctgcacgta gccgtcgatc tccttcgcgt tgatcgcgag
     2221 cgcctttcgg aaatggtcga tggcgatttc gtgcttggcc atgcgctggt acacatatcc
     2281 gatctggaga taggtcttcg agaattccgg caccagattg gcagcgcgcc tgaggtgcac
     2341 gagcgcctcg cgcaggcggt cgagatggat gaggcacatg ccgaggttgt attcgaacgg
     2401 gaagaagagc ggcgcgcggg cgagggcctc gcggaggagc ttctccgcat cggcataatt
     2461 cgcctgttcc atgagggcga tcgcgcggtt gttcgccgct gcggcctcgt tgcttccggt
     2521 gcagtggatg agctcgttct tgtagaagat gcgctcgtag gcctgcgggt cgtccggcgg
     2581 cttttcgatc tggcggaaca gaaagcggtt cgatggatcg agcgtgtcga cgtcgatggc
     2641 gaacagggag cgcgtgggga gcgtcgcgat tcccgaaatg agcgcggcgc atgcaagcgc
     2701 ggcggtgagc cgcacccggc gcaggggagc gcggtcgccc atcagtacag atggcaggcc
     2761 acgcggcggc cgccgacctc cttcatgggc ggcggaacgc tccggcacgt gtccatcacc
     2821 ttggggcagc ggggatggaa gtggcagccc gccggcgggt cctccggcga ggggatctcg
     2881 ccgggcagca ccgcgaatcc cttgcgcgac ggctcggccg tcgggatcga cgtgaggagg
     2941 cttgccgtgt agggatgcag cggttcgcga tagagctcgg tccgggcgct ctcctcgacg
     3001 atcttgccga ggtacatcac cgcgatgcgg tcgctcatgt gctccaccac cgcgagatcg
     3061 tgcgccacga agagaaaggt gagtcccagc ttctcctgca ggtccctgag aagattgagg
     3121 atctgcccct ggatggagac gtcgagcgcc gagaccggct cgtcgaggat gatgaaggcg
     3181 ggattgagcg cgatcgcacg ggcgatcccg atgcgctggc gctgtccgcc gctgaactcg
     3241 tgcgggtatc ggtccgcatg ctcgggggaa aggcccacca gctcgagcag ctcgcgcagg
     3301 cgcgaggcga gctcggattt ctccatcgag cggtggatgc gcagcggctc ggtgatgatc
     3361 ttgcccgcgg tcatgcgcgg attgagcgag ccgtacgggt cctggaaaac gatttgcatg
     3421 ttgcgccgga acgaacgcag ctcggcgggc ggaaggcgca ggacgtcgcg cccgttgatg
     3481 acgacctcgc cggcgtcggg ctcgatgagg cgaagcatcg aacgggccgc cgtggtcttg
     3541 ccgctgccgc tctcgcccac catgcccagg gtctttcccg cctcgatggt aaatccgata
     3601 tcgtcgaccg cgcgcaccac gcccggccgt tcgaaaaaac cgctcttgcg agcgctgaaa
     3661 tgcttcgtga gtcccgcgac gcgtaccatc ggcccatcct ccctgcgact ggccgcatga
     3721 tggggccggg gcgggcgcga gcgcaagaaa aaaataattg cggattttac cgcgcaggct
     3781 cagcatcggt gtgatccgcc cgcggccgcg ccctgccggg gacgcatcgt cgcttccgat
     3841 ggcactatcg aaacaaaaaa ccttctcctt gacctatcag gtcgcctccc ttcacggcac
     3901 gcgaaaggac ctcatcgcat ttctgatgga cgctgcgctg cccgtggacg tcgtctcgca
     3961 gatcgagttg gcggcctacg aggcgctggt caacatcatc gagcacaatc ctcatccccg
     4021 cgccggcgcg aaaatatcca tgcgctgcac catcgccccc gcgcgcatca cgctgaccat
     4081 cgccaatacc ggcgtgcatt tcgatcccac gacggcgccc atgccggacc tcgccgagca
     4141 tttcaagaag ggccggcggc gtgggctcgg gatctacatt atccggacgc taatggatac
     4201 gatggagtat tcgttcgcga agggcgtcaa ccggctgcgg atggtcaagc ggatcgccta
     4261 ggaggcgcgt cccacaatcc ggctctttcc ctgcatgagg aatttcacgc cctcgtcgag
     4321 cccctcgagg gtgagctcga acatggggaa gatgtcgcga accatgcgga tggtctgcac
     4381 gtagtcccac gagcggcggg gcagcggatt gagccagatg gagtcgcgga acttgtcgcg
     4441 cagtcgctgg agccacacga tgccgggggt gtcgttgtgg taccagtagt cgatggcgcc
     4501 gttccgccag gtgagctccg agggggccat ctcggcgtcg cccaccagaa tgagcttgta
     4561 gtcggggtcc tcgctcttga ggagatcggc ggtggagatc gtcgcgtcgc cgcgctcgat
     4621 gtcgcgccag aggtcttggt acacgcagtt gtggaaatag aaatgcttga accggctgat
     4681 ctgcccgctg gccgccgaga acaacaactc caccagctgc gaatagggcg tcatggaacc
     4741 gcccgcgtcc atgagcagga tcaccttggc ggccttcttc tccttcgttt cccagacgaa
     4801 ctcgagctcg cccgcgttct tgcaggtcgc gtcaatggtc tcgcccaggt tgaggcgctc
     4861 ctcgggcccg atgggcaaca gcgagcgcag gtgcgaaagc gccaccttca tctgacgcgt
     4921 gtcgagggta atgttgctgg agtagttccg gaaggtccgc ttctcggcga tttgcacggc
     4981 gcgcttgtgg cgcgactcgc cctggccgat gcggatgccc gcgggattgt agccccacgc
     5041 gccctgggtg gagcggccgc cggttccgat ggccttgttg ccgcccacat gccccttgta
     5101 gtgcccctgc ttgagctgct cctcgaagtt cttcaggacc tggtccaggt ccagctgctc
     5161 catctgccgc ttttcctcct cgctcaggtg cagctccttg actttcttga ggccctcgag
     5221 gatcttgtcg agcatctcgt cggtggtctc gatgtccttg aaggtgtcca ggaaggccag
     5281 gtcgtatcgg tcgaagtgga tctcgttttt cacgaggatc gcgcgcgcga tgtggtaaaa
     5341 cttcgtgagg ctgcagtcgt tgaggttcgc aacgagcgcg tggtgcagcg tgatccactc
     5401 gtgtagcgag accggcacgc catgggcctt gaggttgtag aagaaattaa taaacaccgc
     5461 ggctccgggc gctcgagctg taggcgtagt gcgacttgtc cgagcccgca atcacgtcca
     5521 tatcttcctt ctttttgatg agcgcgccca ggaacgggag ttcgctctcc accttcttcg
     5581 gggagatccc gcccgcgagc agcgcctgta tccagtcgat gagctcgctg gtggacggct
     5641 tcttgcgcag catgtcgaac tcgcggatcc agtagaactt cttgaggcac tccttcagga
     5701 gcttcttgtc caggtccgga aagtgaacgt tcacgatgtc ggtcatgagt tcctgaccgg
     5761 gaaattcgat gtaatggaac acgcagcggc gcaaaaacgg gtcgggcagc tccttttcgc
     5821 tgttggaggt gatgatgcag atgggacggt gcttggcctt gacggtcacg cccgtttcgg
     5881 ggatgtagaa gctcatctcg tcgatctcgt tgaggaggtc gttcgggaac tcgatctcgg
     5941 ccttgtcgat ctcgtcgatg agcaggacca cctgctcctc cgcagcgaac gcctcgccca
     6001 gcttgccgag cttgatgtac tgtttgatgt ccgagacgtc gcggtcgccg aagcgcgcgt
     6061 cgttgagccg ttggacggta tcgtacacgt acagcccctc tttcgccttc gtggtcgatt
     6121 tcacgttcca gatgatgagc tttttgccga tccccttcgc gattgaatgc gccaggagcg
     6181 tttttccggt tcccggctcg cccttgacca aaagcggccg tccgagaacg atggagacgt
     6241 tcacgttgtc gcgcagctcc ggggagagaa cgtagtcgtc agagcccttg aagaaatcca
     6301 tatccctacc tctatcggtg tcgcatatat aatgcggcga ttgcacgctt gcataccttt
     6361 cccgcaacac tattttcaaa gcgcccactg gcaagtattt tttctttacg tttgccgccc
     6421 atatgttcat tatccatgtc aagcgcctct gaacgatccg atgaccatca aggaaatccg
     6481 cccctttccg caatgtgtta agagacatcc tgccggttat gagaaatggg tcacccctgc
     6541 agacagtccc cttccggcca aaaaattcta tacatcggcc ttaaacttac tttttatcgc
     6601 gcataatttt tgttggctat gttcccctac ccggggacaa ggaacaaatt tttttcttta
     6661 caaacttcgt tttatggaag atctgcgggg taaaataatt tttccgggag gcttgaagtt
     6721 atgaaggtgt acttgaagtt attcattgca ggcatggttc tgctgctgtc ctcttgttct
     6781 tatgtcagga ttgtcgacat cttggacgaa tcgagcgcca tcgtttttta tacggaggaa
     6841 ggtgttcagc gtaagaagaa tgccgaaaca gtacctcttg aggttgtccg ttacgaaaaa
     6901 ggacaaaaaa ttccaatttt acgtgtaaaa aaatgggttg ctacctggac aggcatgtcg
     6961 gttatgaaag cggaggaatt cagagcaggt actaaagatg attcttattc cagaacttta
     7021 atcacttctg tccaaaatca aggagacggt attgcgaaaa atctgatggt catagagacg
     7081 atccctgcgc gctttgttgt ggagagcata ggtttcgtcg atgggagcgg gctttatggg
     7141 tattcactag gagtaaagga ctttaataat tacggcatca ttagcaaaag gagcggtccc
     7201 aatggcacca cgatatacga aatcaagatc ctgaagccgc tgaatcccga tcaagtgttc
     7261 agtattgggt tcatgggaca ttataaagta gccgctcgca aatcgatgtg aaggggcgat
     7321 tactgcttcg atcgaaaaaa atatactgca ttttttttac gaggccctgc gtaaaatcgc
     7381 ggggcttcat tttaccatca aagagacccc gcccctttcc gcaatgtgtt aagagacaag
     7441 tgaaataatc caaagcattg cgtggacggg atcgcgcgcc tgcgatcatg gagttttctt
     7501 gatcatctgc aactattcag cacaatattt gcgagggggg agaaaggagt agtagtctta
     7561 accatcaagg aattacgacc gtttcctaga ggaatcaaga gacatgctgc gggtttatgg
     7621 gaactggatc acccctatag acagcccccc cgctggcttc gatacgcctg cggctactca
     7681 gccagcgggg gggctgcttc cacggtagtt cgttactaaa ttattccgca agctgaatac
     7741 ctgcagggac acctgaatag catgatggct gaatagccaa caacatacca gaatggcacg
     7801 gtggttgagt agccgtaggc gtatcgaagc caccgggcca ttctgaagcc cgttacggga
     7861 ctgaggctcc gatatcggca gcggtcacat cccgcaacca gccccttcca ggccgaaaag
     7921 ttttcgtaca ccggctttac atcaattgcg caactacatg gctgtgtcat gagctgagga
     7981 gcaggcataa aatattttca gtagaaaatg ttccttccga gcatatcccg ccagaaagat
     8041 cgtgtgcacg agctacgata gtctctgtca tcgcgatgga gaaaatatgc ttgcgtacga
     8101 ggcgattagc atggcgaacc gcacccgata cataaggcag aatacggact ggccgcattg
     8161 tgctcccgca gtagggacag acctcgaggt ttttcatcga atgaattctc gactgcgaag
     8221 aaacaggttg agggtcggtg aaaaacagaa cgggcacccg ccgggtgccc gttcgatgca
     8281 tcgaacgatg atgacgatcc tgccgtcagt accccttcga cttcaactcg cccacgatat
     8341 tcacgaagaa cgcgggggcg tcgaaaccgg gcgtaatgcc gaggaactgg tcgacgatgt
     8401 tgaagtgcga tacgccgccg caccaccagg cgccgctctc gatgccgcgg aagttgcccc
     8461 acttggccga attgaccgac accaggcagt cattgtcccc ctcatagtac ttaaggagaa
     8521 ggtaggtcgc cccggtcagc aggaaatcgg cggtgaccag cttgatcttc gccgcccagc
     8581 tctggtagta gatccccgcc tggttcggcg tgttgggatt gaagacgccg ttcatgtacg
     8641 acatgcgaag atcgtacgcg ccgcccttca tgtcggggtt ggtgtcgccg agcagccaca
     8701 tcgcgatggt attgttgatg ccggtgacga gccattcgcc ccagtcggga agcacgccga
     8761 tgagaacgtc ggcgacggcg ctcccccggt ggggacccgc gatgctggta tagctggcga
     8821 ccttgtcctt catgccaagg ttcgcgatca tatagcgcga atcgagggtc ccgtgggagt
     8881 gaccgatgat attcaccttg gatttgcccg ttacggccag tacatagagg acatacttct
     8941 tcaactcggc ggcgcgatcg gcggtcctgt cataggcctg ctgatccgat atgaacacct
     9001 gcgctccctc atcgcggagt tcgccgggga tattgtacca gtacccgatc ccgagattgt
     9061 tcgcccgtgc gcccatccca tgcgagagca cgatcggata tttcgtcgag catttgctgc
     9121 tcgatccgcc agcgaacgca atgaccggaa ccaacaaagc gataatgcaa aaagcgatta
     9181 ctacccgttt catgaatcct cctattgaat actcaccctt cgattattta tgacagaacc
     9241 atatttaact gtcggcttaa tgtcaataaa aaagttaaac cgtacggtcg gtataaaata
     9301 aaaaagtgac gatttcctaa ttattatata ctttaagccc tataaagtac ttattccatt
     9361 tcatttttct cggaattgat tcagacgacg taatcggttg ctgcacgagt aggcagcacg
     9421 cattagccgc cagaactgca gggatacatt aaacgattat atgcaaatag tgcaatgctg
     9481 gggatagaag ggacctggaa ttatgcgcga ccgaatgagc gtgactgccc atcggctctt
     9541 ttgacttaca tgagagggaa gctcttaatg cactatcatc ccggtgataa aaccgttcag
     9601 ttcttctgcc gaaaaacgta tgcggccata aagaatatcc caacactgat cgtgaccgtt
     9661 aaagctgatc tctttcggtt ttccaagggc gatgacgagc tcttcgagta tgatacgttc
     9721 cgtcttcaga tcatacatga aacccgatac gggttgtgcg ggattattat gcggcagcac
     9781 gatgatcagg gtaaagcgcg aatgcgagat cgcattggcg cccatccatg cgtcggatat
     9841 tctcggcgct cccaggatgg cgatcaccgt agactctggc attcccagcg tgatttcggg
     9901 acgatgatgg cagtagccgt ttcgtgccga tttgaaatac gcctggataa tcgggacatg
     9961 ctccggaacc gatctctttg gcctttcata cgtgagaaat ccgccaaata tatatccggt
    10021 tttacccgag aacgacgccc tatgccaacc ggcatcgatg ccatcaatcg tttcccgtgc
    10081 cccgacgtct gcaataaccg tgatctccgc aagaaacgga atcacgagga tcgacgcgtt
    10141 ccttcgggaa gggcccgtcc ctgagaagaa ggcctgccgg tgacatcccc atgcccgcga
    10201 tggcaggtcg cgcgcgatcg atacgctcgc aatcggaaaa aaaccgccat cgtggtgaca
    10261 atggcagctc cccataccat gccgctgaat cgcttcattc agttatgcac ccgcgcgccg
    10321 ccctcgggat tgcgtcattg tcggccgtag gctaccgggc ctgcttcgta tagttgagca
    10381 gtccccccgc cagcaggatc tcgcgctggc gttcgctggc gacgatctcg agctcgcaat
    10441 ttttcccgcc gattttcacg gtgaccgtct tcatgctttt cacggcctcc cggaggttcg
    10501 agacggtgaa tgcatcgccc ttcacgatcg catcatagtc cgtaggattg gcgaagacca
    10561 gcggcacgat cccgaagttc accaggttgg ccgagtggat gcgctcgaac gatttcgcaa
    10621 tcaccaggcg cacgccgagg tgcatggggc agatggccgc gtgctcgcgg ctggatccct
    10681 gcccatagct ctgtcccgcc acgacggcgg tgtacacgcc cttctgcttg ttcgcgagcg
    10741 ccgccgccgc gaaggcggga tcgaccccct caaagacgaa ttgcgcgtat ttcgggatat
    10801 tcgaccggta cttgagccgc gcgccggccg gcatgatatg gtcggtggtg atcttgtcgc
    10861 ccaggcgcgc gcccacgacg cctgcgatgg aatccaagag cggctcggtg tagggcggct
    10921 ccccgatatt gggcccgcgg aaaatatcca cgggcgtcgg cgatggcgga acgatcatgg
    10981 agtcatcgac gatgaagcga tcgggcacgg caacagaggg atactccgcg acggggcgtt
    11041 ccagcggatt gacgagctct ccggc
//
LOCUS       DAORHF010000113        10964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5216_length_10964_cov_6.083692, whole genome shotgun sequence.
ACCESSION   DAORHF010000113 DAORHF010000000
VERSION     DAORHF010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5216_length_10964_cov_6.083692"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..689
                     /gene="efp"
                     /locus_tag="PKJ16_10475"
     CDS             126..689
                     /gene="efp"
                     /locus_tag="PKJ16_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV47456.1"
                     /translation="MVTTNDLRRGMVIKLDGELYTVVDQNHHKPGKGGAIVRTKLRNV
                     KKGTVKDMTFRPSEKVEDVRLDQRPMQYLYEEGDHLVFMDTETYEQESIPTDAIGDAA
                     SFLKPEDICEIAIYEGKPITVTPPSTVVLKVTYAEPGAKGDTATNVTKPVKVETGAEV
                     KVPLFINEGDYIKVSTETGAYMERVKK"
     gene            complement(722..1072)
                     /gene="ssrA"
                     /locus_tag="PKJ16_10480"
     tmRNA           complement(722..1072)
                     /gene="ssrA"
                     /locus_tag="PKJ16_10480"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1138..1494)
                     /locus_tag="PKJ16_10485"
     CDS             complement(1138..1494)
                     /locus_tag="PKJ16_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HNV47457.1"
                     /translation="MYAITIAIGLGAGVLGGLVGIGGGIIIVPLLVFGLHFDQHLAQG
                     TTLAAMIPPIGLLAAIEYYRNGHVDIGIAAIIAVSFVVGGYAGSRFAIAIDASILQKV
                     FGVVLILAGVAMLIKK"
     gene            1621..2148
                     /locus_tag="PKJ16_10490"
     CDS             1621..2148
                     /locus_tag="PKJ16_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47458.1"
                     /translation="MIARPAPFIASLLIIACAIQSVHSRPATKARYAGWKPFEGAWFE
                     ISYPPGFTPRPSKKSATSERGCDSAFFLSPDKLVEFYVFSPQWSGEPDDIRPDPATET
                     VASHKQERTDGRTTRWVEIRAKNRAYLRAYADTVDETRNTRLVFGIKYRDERAYGRYK
                     DTYLAFKQSLVQFAD"
     gene            complement(2164..3384)
                     /locus_tag="PKJ16_10495"
     CDS             complement(2164..3384)
                     /locus_tag="PKJ16_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47459.1"
                     /translation="MLVGLLASVAGASTTVKISPDPALSGFGATIDTTAAEKQFAKYI
                     DTFFEKNRLVFPSGLAMVNTLGYPIGKSYIGQFPSFEFGTAAGLGVFQRHRQDDYTDT
                     NPTMPGGGANGGFHIGTGITDDLDIILKFFSLNWVYRVDETFENQGDTTQFIFYAKDI
                     NFYSFGAKLRYNIIDPMSLVPFVLKFGGVTVGCGLDYFRMTANTSMDITDTRDVSVDI
                     LGTNYSTSAVANYSSDVTFTADIVSVTPEIFVYADLFYFITLYTGPSCSFNWGSLNFK
                     MDGAGSLANSATIGGIVPANTQLATMTIVSDYKMGPYFVIPKWVVGAEFNFWVLKLQA
                     EVSMALTGTNTDIADSMMAQVGVRFHFQGDKEPKRSDDDYEERVKKRDERKKKEKRPL
                     DRNRKYEFIEDYIH"
     gene            complement(3505..6087)
                     /gene="clpB"
                     /locus_tag="PKJ16_10500"
     CDS             complement(3505..6087)
                     /gene="clpB"
                     /locus_tag="PKJ16_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HNV47460.1"
                     /translation="MRMDKLTLKAQDILQSAQNIATNSNHYEIQPEHLLKALIDQEGG
                     IFQTVAQKMGVHAFDLATETDKALASLPRQVGAGPGGGVMSASMRDVLNEAWNEAVRL
                     KDEYLSTEHIILALAAKGDSAARKILNAHGFTKDRVLKVLMDIRGSKRATDENAEEKY
                     DALERYSRDLTKFARMNKLDPVIGRDEEIRRVMQVLTRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIIDGDVPENLKGKRLVALDMGALIAGAKFRGEFEERLKAVITEVEEAGGEVI
                     LFIDELHTLVGAGAAEGSMDASNMLKPALARGELRCIGATTLNEYQKYIEKDKALERR
                     FQPVFTKEPSVEDTVAILRGLKEKYEVHHGVRIADSAVVAAAMLSDRYITDRFLPDKA
                     VDLIDEAASVIKMEIDSMPIEIDEIERKIRQLTIEREAVKKEKDAASKERLAKIEEEL
                     ANLNEKKNELVGQWKNEKNVITQIRQIKESIEQLRIEEQKAEREGNLNRVAEIRYGKI
                     TELEKRMAELNKKLAEMQKDRSLLKEEVTEQEIAEVVSRWTGIPVSRMLEGEMEKLLR
                     MESYIQKRVVGQDEAITAIADAVRRSRSGMQEPNRPIGTFIFLGPTGVGKTETAKAVA
                     EFMFNDERAVIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGQLTEAVRRRPYAVILFD
                     EIEKAHHEVFNIFLQILDEGRLTDAKGRTVDFRNALIIMTSNIGTVYISDASVPMEKR
                     QQGVFEELKMQFKPEFLNRVDEIIIFNPLTKENIRNIVKLQLAMLARRLKEQRNIAIE
                     VTKRAVDFLVETGFDPQYGARPLKRAIQRHILNPMSVKLLGSEIAADASVKIDSDGKS
                     VIFG"
     gene            complement(6203..7879)
                     /locus_tag="PKJ16_10505"
     CDS             complement(6203..7879)
                     /locus_tag="PKJ16_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47461.1"
                     /translation="MKRLLCAVLLSLLAAGAARAQVAVIPYKVENPSSYFAPSNGAEY
                     AKLLALALAIAKEVDVASPRDTVLDLQRNAIDPQGVLTGEGLQALAKGGYVDFFLLGK
                     MKKTATGYASDSVLYSVRERRVVSSCASRAKTLVGLAERDVKGVFTQFRDRAAGAPRA
                     SRVDAVIVLDTSYAVLREWRSLIEGVQAFAGAISDNWNLDTRLSVLPFSKHCPRVNRA
                     LNIATPTGLSKALGALAPKGADGEACLAEALSFAARSISWREGSTRAMLLVTNTPIRN
                     KGFLERHLIAAKRKGVRTVTLSLGRLSASETAAVKQLAIVGGGRHVNATYHQRLYDID
                     GRPLDIFLEAGRLFQSRGFEDWRQGLFAKRAGSNTGYAKPDAGLEEFFYSEKKRSVDP
                     YSMAEHFGRLSKTPTVRAETVESNIESALVRAGGALPVKRGSRRAARQLGKALISDGA
                     VSLWVRITTEKDLAFFREKSSSRFFFPLGVSLQKKADEPYGFAINSERMVTNITADFV
                     PDVVSAKLGDMVKNPAAFMGKGLFRPPVWFITVKVEEFEGETRGTDIRDR"
     gene            complement(7930..9084)
                     /locus_tag="PKJ16_10510"
     CDS             complement(7930..9084)
                     /locus_tag="PKJ16_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47462.1"
                     /translation="MDDVTIIAYPGFRSTSLLSLVEGRSRYMVPFGGKFRVVDFLLGG
                     ALASNARRTIIYNNYDDDLEEYVERHGPFKGQEFPRVKVMSREQNDTRVCYELIMDSN
                     TGYYIIYNGDHPAIIDFPLLMRRYHALRKNAVLFKLKFGGSATMAHTCLVTDQASILA
                     AVNTAIEEGRQSPGLFEMIINLVQIKGVEVSEFPVEYWPLKRVTDYYFINHAIFRTPD
                     LFNLLFRTSSIQSRIEIERYANLGRFANISNAYISDGCDINGTVTNSIIFPGVVIGEK
                     SIVRDSIILPHVRIGDGSTVVRTVIDERTEPAETGLNVGHRCRVGSEEENIRNSDYSR
                     TISRGITLIGRNARIQDGSNIGGACYVAPGVVPGGPEGRITLYNGLSVVK"
     gene            9149..10681
                     /locus_tag="PKJ16_10515"
     CDS             9149..10681
                     /locus_tag="PKJ16_10515"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNV47463.1"
                     /translation="MTHRTLTVLPGGRTGAFPDGTLLIDALADLGAFPPTPCGGKGIC
                     GKCAVRVDMPAAEGPAGRSIETALACRTHLTRDLVVHVDRDPTARIRPPARLDPRARY
                     GVAVDIGTTAIKIALADLDAERAVETDSFLNPQRRFGDDVIARITAAADESVRRRLAG
                     LVRDAVATSVARTLADAGIAPDAVRTIAFSGNTVMLYLLAGLPVETLGAYPYRTPWRD
                     IPLPPEETPLIPAFAHATSFFFPIASAFLGGDLVGGLALTLADGIDRNAFFIDLGTNG
                     EMFLIDANGAVTATSCAMGPALEGMNISCGMTADEGAVVHCEPRGDGFDLEVLGGGPA
                     RGISGTGLVDCAALLVRTGAITNAGRIDPEAPRTLPAGLRIEERDGMRRVRIAEGVAI
                     TQRDVRNLQLAKGASLAAARILLDETGTDPADITTVIVAGTLGTRVNMDAFRRLGFLP
                     DYPVAAYCASGNTSLAAAARACRDAAFRDSLRAARDRIRVVELSDRPDFNDRFLASLN
                     FA"
     gene            10753..>10964
                     /locus_tag="PKJ16_10520"
     CDS             10753..>10964
                     /locus_tag="PKJ16_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47464.1"
                     /translation="MTPVYCIAEDPAPLRDTLSRRGIDAIALPLCDIDFAGPRGAAPA
                     VPGVAVVELCDRSRAARASRGTTSRWT"
BASE COUNT         2024 a         3586 c         3264 g         2090 t
ORIGIN      
        1 gattgcctgc tcgtcgtaca cgaaatacaa gtacatcgtc acccagacct gctcggccac
       61 gcaaaagtgc gtcgccgctg cggacggcaa gtcggcgagc tgcaagtaaa tatcgagggg
      121 ccccaatggt tacaacgaac gacctcagac gcggcatggt catcaaactg gacggcgaat
      181 tgtatacggt ggttgaccag aaccatcaca aaccgggcaa gggcggcgcg atcgtccgga
      241 ccaagttgag aaacgttaag aaggggacgg tcaaggacat gaccttccgg ccctccgaaa
      301 aggtcgagga cgtgcggctg gatcaacggc ccatgcagta tctctacgaa gagggcgacc
      361 acctggtttt catggacacc gagacgtacg aacaggagtc gattcccacg gacgccatcg
      421 gcgacgcggc gagcttcctg aagccggagg acatctgcga gatcgccatc tacgagggga
      481 agccgatcac cgtgaccccg ccgtcgaccg tcgtcctcaa ggtaacctac gccgaaccgg
      541 gcgcgaaggg agacaccgcg accaatgtca ccaagccggt gaaggtcgag acgggcgccg
      601 aggtgaaggt ccccctcttc atcaacgagg gcgactacat caaggtaagc accgagaccg
      661 gcgcgtacat ggagcgcgtg aaaaaataac aaaaaagggc aggatttgca tcctgcccgt
      721 actgtggaga cggcgggatt cgaacccgcg tcctgcaacg catatccgaa gcctctacat
      781 gcttatcccg ttcttttaat ttaagccctt tgaccgggaa cgggaaccgt tcgcggggcc
      841 gtgttccctg ttagttcgcg tctcccccgg gaacgaggtt cgaagctatc ccctgtcgtt
      901 ggcgccccgg gcgattccag aggagcggat catcccggaa cgtcgcggct tacgccgcga
      961 gtgctagact attgtctgca gttactttag gttccgcctt ttttacgagt cagcggagaa
     1021 ctcggcatgc agctcacgga cacaccataa cagtcgaaac cagatcgtcc ccgtgtatcc
     1081 agcaaaggaa tatacatacg atttcgccgc gcgtcaagtg aaaaggacgg ctttgtgcta
     1141 tttttttatc agcatcgcga cgcccgcgag gatcagcacg acgccgaaca ccttctgcaa
     1201 gattgatgcg tcgatcgcga tcgcgaacct cgatcccgcg tatccgccaa cgacgaacga
     1261 taccgcgatg atcgcggcaa ttccgatatc cacatggccg tttcgatagt attcgatcgc
     1321 cgcgagcagt ccgatgggcg ggatcatcgc cgccagcgtc gtaccctgcg cgaggtgctg
     1381 atcgaagtgc aatccgaaca cgagcagcgg aacgatgatg atgccgcccc cgatgccgac
     1441 aaggccgccc aacaccccgg cgccgaggcc gattgcgatt gtgattgcgt acatggttcc
     1501 tccaaaatgt tttgacgtcg ctggcgtcca tcgtgtacac tggaggcaga gatgatggcg
     1561 cccgcgtttc attcatcgaa gcgccgggat ccgtcacatc caatcgaaca cggagacgcc
     1621 atgatcgccc gacccgcgcc gttcatcgcc tcgctgctca ttatcgcctg tgcgatccag
     1681 tcggtccatt cccgtccggc caccaaggcg cgctatgccg gctggaagcc gttcgagggc
     1741 gcatggttcg aaatcagcta cccgccggga ttcacgccgc gaccgtcgaa gaaaagcgca
     1801 acctcggagc gcggttgcga cagcgcgttt ttcctctcgc cggacaagct agtggagttt
     1861 tacgtgttct ccccgcagtg gagcggcgag ccggacgata tccgcccgga tccggcgacg
     1921 gaaacggtcg catcgcacaa gcaggaacgc acggacggcc ggaccacccg ttgggtcgag
     1981 atccgcgcga aaaacagggc gtacctgcgc gcctacgccg atacggtgga cgaaacgcgc
     2041 aatacgcggc tggtgttcgg gatcaagtac cgggacgagc gcgcgtacgg ccgctacaag
     2101 gacacgtatc tggcattcaa acaatcgctg gtgcagtttg ccgactgacg gcgtgacgcg
     2161 cggtcagtgg atataatcct cgatgaactc gtacttgcgg ttccggtcca gcgggcgctt
     2221 ttccttcttt ttcctttcat cgcgcttttt cacgcgctcc tcgtaatcgt cgtcgctgcg
     2281 cttcggctcc ttgtcgccct ggaaatgaaa gcgtacgccc acctgcgcca tcatgctgtc
     2341 ggcgatgtcg gtgttggtgc cggtgagcgc catcgacacc tcggcctgca atttgagcac
     2401 ccagaagttg aactccgcgc ccaccaccca cttggggatc acgaagtagg ggcccatctt
     2461 gtagtcggag acgatggtca tggtggcgag ctgagtattc gccggaacga taccgccgat
     2521 agtcgcgcta ttggccagcg aaccggctcc atccattttg aaatttagcg agccccagtt
     2581 gaaagagcag gaggggccgg tatacagcgt tataaaatag aataaatccg catagacgaa
     2641 aatctctggc gtcaccgaca cgatatccgc agtaaaagtg acatcgcttg aataattcgc
     2701 aacggcggat gtcgaataat tcgttccgag aatgtccacc gacacgtccc gggtatcggt
     2761 gatatccatg ctggtattcg ccgtcatcct gaaataatcc agcccgcagc caacggtgac
     2821 accgccgaat ttcagcacaa acggtacgag cgacatcgga tcgatgatat tgtagcgaag
     2881 cttcgcgcca aaagaataga aattgatgtc ctttgcgtaa aagatgaact gcgtcgtgtc
     2941 gccctgattc tcaaaggtct cgtcgacgcg atacacccaa ttcaaggaaa agaacttcaa
     3001 gataatatcc aggtcatcgg taatgccggt cccgatatga aaaccgccgt ttgccccccc
     3061 accgggcatg gtgggattgg tgtcggtgta atcgtcctgc cgatggcgct gaaacacccc
     3121 caaaccggcg gcggtgccga attcgaacga agggaactga ccaatgtacg attttccgat
     3181 cgggtagcct agtgtgttaa ccattgcgag gccgctggga aacaccagcc ggttcttttc
     3241 aaaaaaggtg tcgatgtatt tcgcaaattg cttctctgct gcagtggtgt ctatcgttgc
     3301 accaaaccct gagagagctg gatcgggcga tatcttcacg gtcgtcgacg cgccggcgac
     3361 ggacgcaagc aggccaacca acacaacgac acaccacgag cgtacgacac atcgcataat
     3421 catcaagcat gcctcttgca aagggggcgc gtttgcggca cgccccctac aaccccatga
     3481 gtatacttat ttatcgggaa gaagtcaacc gaagattacc gatttaccgt cgctgtcgat
     3541 cttcaccgag gcgtcggcgg caatctcgct cccgaggagc ttcaccgaca tggggttcag
     3601 gatgtgcctc tggatcgcgc gcttcagcgg ccgggcgccg tactgcggat cgaagccggt
     3661 ctccaccaga aaatccaccg cccgcttggt gacctcgatc gcgatgttcc gctgctcctt
     3721 gagccgccgc gcgagcatcg caagctgcag cttcacaatg ttcctgatgt tctccttcgt
     3781 gagcggatta aagatgatga tctcgtccac ccgattgaga aactccggct tgaactgcat
     3841 tttaagctcc tcgaacacgc cctgctggcg cttctccatg ggaacactcg catcgctgat
     3901 gtagacggtg ccgatgttcg aggtcatgat gatgagcgcg ttgcggaaat ccaccgtgcg
     3961 ccccttcgcg tcggtgagcc gtccctcgtc caggatctgc aggaagatat tgaacacctc
     4021 gtggtgcgcc ttctcgatct cgtcgaagag gatcaccgca tagggacgcc gccgcaccgc
     4081 ctcggtgagc tgcccgccct cctcgtagcc cacgtagccg ggcggcgccc cgatgagccg
     4141 cgcgaccgcg tgcttctcca tgtattccga catgtcgatc cggatgaccg cgcgctcgtc
     4201 attgaacatg aactcggcca cggccttcgc ggtctcggtc tttcccacgc ccgtgggccc
     4261 caggaaaatg aaggtcccga tgggccggtt cggctcctgc atccccgagc gcgaacgacg
     4321 cacggcgtcg gcgatcgcgg tgatcgcctc gtcctgcccc accacgcgct tctggatgta
     4381 cgactccatc cgcaggagct tctccatctc gccctcgagc atccggctta cggggatgcc
     4441 cgtccagcgc gagaccacct cggcgatctc ctgctcggtc acctcttctt tcaggagcga
     4501 gcgatccttc tgcatctccg cgagcttctt gttgagctcg gccatgcgct tctcgagctc
     4561 ggtgatcttt ccgtagcgta tctcggccac gcggttgagg ttcccctcgc gctcggcctt
     4621 ctgctcctcg atgcgcagct gctcgatcga ctccttgatc tggcggatct gcgtgatcac
     4681 gttcttctca ttcttccact gccccacgag ctcgttcttc ttctcgttca ggttcgcgag
     4741 ctcctcctcg atcttcgcca gccgctcctt cgacgcggcg tccttctcct tcttcacggc
     4801 ctcacgctcg atggtaagct ggcgtatctt tcgctcgatc tcgtcgatct cgatgggcat
     4861 cgagtcgatc tccatcttga tgacggaggc cgcctcgtcg atgaggtcca ccgccttgtc
     4921 ggggaggaaa cggtcggtga tgtagcggtc cgagagcatc gccgccgcca ccacggccga
     4981 gtcggcgatc cgcaccccgt ggtgcacctc gtatttctcc ttgaggccgc gcaggatggc
     5041 cacggtgtcc tccacgctcg gctccttggt gaagaccggc tgaaagcgcc gctcgagcgc
     5101 cttgtccttc tcgatgtatt tctggtattc gttcagcgtg gtcgcgccga tgcagcgcag
     5161 ctcgccgcgc gccagggcgg gcttgagcat attggaagcg tccatcgacc cctcggccgc
     5221 gcccgcgccc accagcgtgt gcagctcgtc gatgaagagg atcacctcgc ccccggcctc
     5281 ctccacctcg gtgatcaccg ccttgaggcg ctcctcgaac tcgccgcgga acttcgcccc
     5341 ggcgatgagc gcgcccatat ccaacgcaac cagacgcttg cccttgaggt tctccggcac
     5401 gtccccgtcg atgatccgct gcgcgagccc ctcaacgatg gccgtcttcc cgacgcccgg
     5461 ctcgccgatg agcaccggat tgttcttggt gcgccgcgta aggacctgca tcacgcgccg
     5521 tatctcctcg tcgcgcccga tcacgggatc gagcttgttc atgcgcgcga acttcgtgag
     5581 atcgcggctg tagcgctcca gagcgtcgta cttctcctcg gcgttctcgt cggtcgcgcg
     5641 cttggacccg cggatgtcca tgaggacctt gaggacccgg tccttcgtga agccatgggc
     5701 gttcaggatc ttccgcgccg ccgaatcgcc cttcgcggca agggcaagga tgatgtgttc
     5761 ggtgctcagg tactcgtcct tgaggcgcac cgcctcgttc cacgcctcgt tcaaaacatc
     5821 gcgcatcgag gccgacatga cgccgccgcc gggacccgcg cccacctgcc gcggcaggct
     5881 cgcgagcgcc ttatcggtct ccgtggccag gtcaaatgca tgcacgccca tcttctgcgc
     5941 gacggtctgg aagatcccgc cctcctggtc gatgagcgcc ttgagcaggt gctcgggctg
     6001 gatctcgtaa tggttgctgt tcgtcgctat gttctgcgcg gactgcaaaa tgtcctgcgc
     6061 cttgagtgtg agtttgtcca tccgcatggg tcttcctccg cacaacagtt ctgctatcgg
     6121 tgttcaggat aacggggagt aattaatcac gcaggcgtta atcgatcaaa aatttttttc
     6181 aaatatgtaa tcggctgccc tatcagcggt cccggatgtc cgtgccccgc gtttcgccct
     6241 cgaactcctc gaccttcacg gtgatgaacc acaccggcgg ccgaaacagg cctttcccca
     6301 tgaaggccgc ggggttcttc accatgtcgc ccaacttcgc gctcacgaca tccgggacga
     6361 aatccgcggt gatgttcgtg accatccgct cggaattaat cgcgaagccg tacggctcgt
     6421 cggccttctt ctgcagcgac acgcccagcg gaaagaaaaa ccgggacgac gatttctccc
     6481 tgaaaaacgc gaggtccttt tcggtggtga tgcgcaccca gagcgacacc gccccgtcgc
     6541 taatcaacgc cttgcccagc tgccgggcgg cccgacgcga accgcgctta accggcaggg
     6601 cgccgcccgc ccggacaagg gctgattcga tattgctctc gaccgtctcc gccctcacgg
     6661 tcggcgtttt ggaaagccgc ccgaagtgct cagccatgga gtacgggtcc acgctgcgct
     6721 ttttctcgct gtaaaagaat tcctccaggc cggcgtcggg cttggcatat cccgtgttcg
     6781 atcccgcccg cttcgcgaag agtccctgac gccagtcctc gaatccgcgc gactggaaga
     6841 gccgcccggc ctcgaggaag atatcgagcg gcctgccgtc aatatcgtac aaccgctgat
     6901 ggtaggtcgc gttcacgtgc cgcccgccgc cgacaatcgc gagctgcttc accgcggcgg
     6961 tctcggaagc ggagagccgc cccagcgaca gcgtcacggt ccgcacgccc ttccgcttgg
     7021 cggcgatgag atgccgctcc agaaatccct tgttcctgat cggggtgttg gtgacaagaa
     7081 gcatcgcgcg cgtcgatccc tcgcgccagg atatgctgcg cgcggcgaat gaaagcgcct
     7141 ccgcgaggca tgcctcgccg tccgccccct tcggggcgag cgcgcccagc gccttcgaaa
     7201 ggccggtggg cgtcgcgata ttgagcgccc ggttcacccg cgggcaatgt ttcgagaagg
     7261 gcagcacgct cagccgcgtg tcgaggttcc aattgtccga gatcgccccg gcgaaggcct
     7321 gcaccccctc gatgagggag cgccattcgc gcagcacggc gtaggaggtg tccaggacga
     7381 tcacggcatc gacgcgcgag gcccggggcg cgccggcggc gcgatcgcgg aactgggtga
     7441 acacgccctt cacgtcgcgc tcggccaacc cgacgagcgt tttcgcgcgc gaggcgcacg
     7501 acgagaccac gcgccgctcc cgcaccgagt acaggacgct gtcgctggcg tatccggtcg
     7561 cggtcttttt cattttcccg aggaggaaaa aatcgacata gccgcccttc gcgagcgcct
     7621 gcagcccctc gcccgtgagc acgccctgcg gatcgatcgc attgcgctgc aggtccagca
     7681 ccgtgtctcg cggcgaggcg acatcgacct ccttggcgat tgcaagcgcg agggcgagca
     7741 gcttcgcgta ctccgccccg ttcgagggcg cgaaatacga cgaggggttc tcgaccttgt
     7801 aggggataac ggcgacctgg gcgcgcgccg caccggcggc caggagagaa aggagcacgg
     7861 cgcagagcaa tcgtttcatt gatggtccct ctccgcgcag cgcgaccgcg atgggtggcg
     7921 caccgcgggt tacttgacga ccgaaagccc gttatagagg gtgatccggc cctccgggcc
     7981 gccggggacg acgccgggcg ccacgtagca cgcgccgccg atattggagc cgtcctggat
     8041 acgcgcgttc ctgccgatga gcgtgatccc gcgcgagatg gtgcgcgaat aatcgctgtt
     8101 gcgaatattc tcctcttccg aaccgacccg gcagcggtgc cccacgttca ggcccgtctc
     8161 cgcgggctcg gtgcgctcgt caatgacggt gcggacgacc gtgctcccgt ctccgatgcg
     8221 cacgtgcggc aggatgatgg aatcgcgcac gatcgatttc tcgccaatca ccacgcccgg
     8281 aaagatgatg gagttggtca cggtgccgtt gatgtcgcac ccgtcggaga tgtaggcgtt
     8341 cgagatattg gcgaagcgcc ccaggttcgc gtaccgctcg atctcgatgc ggctctggat
     8401 cgacgaggtc cgaaagagaa gattgaaaag gtcgggcgtc cggaagatcg catggttgat
     8461 gaagtagtag tcggtcacgc gcttgagcgg ccagtactcg accggaaact cgctgacctc
     8521 cacgcccttg atctgcacga gattgatgat catctcgaag agccccggcg actggcgccc
     8581 ctcctcgatg gcggtgttca cggccgccag gatgctcgcc tggtcggtca ccaggcaggt
     8641 gtgcgccatg gtggccgagc cgccgaactt gagtttgaag agcacggcgt tctttctaag
     8701 cgcgtgatag cgccgcatga gcagcgggaa atcgatgatc gccggatggt cgccgttgta
     8761 gatgatgtag tagccggtgt tcgagtccat gatgagctcg taacacacgc gcgtatcgtt
     8821 ctgctcgcgg gacatcacct tcacgcgcgg gaactcctgg cccttgaagg gcccgtggcg
     8881 ctccacgtac tcctcgagat cgtcgtcgta gttgttgtag atgatggtcc gccgcgcgtt
     8941 cgaggcgagc gcgccgccga gcaggaagtc cacgacccgg aacttgcccc cgaaggggac
     9001 catatagcgc gagcgcccct ccaccaggga gaggaggctg gtgctccgga aaccgggata
     9061 tgctatgatg gttacatcgt ccatggaaaa tcgtttgaga agaaaaatca gggttgattt
     9121 ttggagaaga aaaatcaata aaaaaattat gacgcaccgc actctcaccg ttttgccggg
     9181 cggacgcacc ggcgccttcc ccgacgggac gctcctcatc gacgccctgg ccgatctggg
     9241 cgcgttcccg cccactccct gcgggggaaa gggcatctgc ggaaaatgcg ccgtccgcgt
     9301 ggacatgccc gctgcggagg gaccggcggg acgctcgatt gaaacggcgc tcgcctgccg
     9361 cacgcatctc acgcgcgacc tcgtcgtgca tgtcgatcgc gacccgaccg cgcgcatccg
     9421 tcccccggcc aggctcgatc cccgcgcgcg ctatggggtc gccgtggaca tcggcaccac
     9481 cgccatcaag atcgccctgg ccgatctgga cgcggagcgc gccgtcgaga ccgactcttt
     9541 cctgaacccg cagcggcgct tcggcgacga cgtgatcgcc cgcatcaccg ccgccgccga
     9601 cgagagcgtg cgccgccgcc tcgccgggct cgtgcgtgac gccgtcgcca cgtccgtcgc
     9661 gcgcaccctc gccgacgcgg gcatcgcccc cgacgccgtc cggacgatcg cgttttcggg
     9721 caataccgtg atgctctacc tcctcgccgg gctcccggtc gaaacgctcg gcgcgtatcc
     9781 ctatcgcacc ccatggcgcg acatccccct cccgccggag gagacgccgc tcatccccgc
     9841 gttcgcgcat gcgacgtcgt tttttttccc catcgcttcg gccttcctcg gcggcgacct
     9901 cgtgggcggt ctcgccctca cgctcgcgga cgggatcgac cggaacgcct tctttatcga
     9961 tctgggcacc aacggcgaga tgtttctcat cgacgcgaac ggcgccgtca cggcgacctc
    10021 gtgcgccatg gggcccgcgc tggagggcat gaacatatca tgcggcatga cggccgacga
    10081 gggcgccgtc gtccattgcg aaccgcgcgg cgacgggttc gacctcgagg tgctgggcgg
    10141 cggacccgcg cgcgggatct ccggcacggg gctcgtcgat tgcgccgcgc tcttggtgcg
    10201 aacgggcgcg atcacgaacg cgggacggat cgaccccgaa gcaccgcgca ccctgccggc
    10261 cggcctgcgc atcgaagagc gcgatggaat gcgccgcgta cgcatcgcgg agggcgtcgc
    10321 gatcacccag cgggacgtgc gcaacctcca gctcgccaag ggcgcgagcc tcgccgccgc
    10381 gcgcattctc ctcgacgaga ccggaaccga ccccgccgac atcacgacgg tgattgtcgc
    10441 cgggacgctc gggacgcggg tgaacatgga cgccttccgc cgactcggct ttctccccga
    10501 ttacccggtt gccgcgtatt gcgcttcggg aaacaccagc ctggccgccg ccgcgcgcgc
    10561 ctgccgcgac gcggcctttc gcgacagcct gcgcgccgcg cgtgaccgca ttcgggtcgt
    10621 ggagctttcc gatcgtccgg acttcaacga ccgctttctc gcatcgctca atttcgcata
    10681 ggcgcgccac gccgcgcgcg tattgttgtt gattatttta ccggcccgcg cgaaaatgag
    10741 acataatacg ccatgacgcc cgtctattgc atcgccgagg accccgcgcc ccttcgcgac
    10801 accctgtcgc gaagggggat cgacgccatt gcgctgccgt tatgcgacat cgattttgcc
    10861 ggtccccgcg gagccgcccc agcggtcccg ggcgtcgcgg tggtcgagct gtgcgaccgg
    10921 tcgcgcgcgg ccagagcctc acgtgggacg acgtcgcgat ggac
//
LOCUS       DAORHF010000114        10869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5290_length_10869_cov_5.334474, whole genome shotgun sequence.
ACCESSION   DAORHF010000114 DAORHF010000000
VERSION     DAORHF010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5290_length_10869_cov_5.334474"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..407
                     /locus_tag="PKJ16_10525"
     CDS             117..407
                     /locus_tag="PKJ16_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMG-box domain-containing protein"
                     /protein_id="HNV47465.1"
                     /translation="MATVKFTSIIQHASGQFGGIVFSHRKGKPYLKRYAKPRDPRTAA
                     QVAARERFRECAAKWRTLPPEEKAAWKARARRENATGYNLFMREGMRDRAGA"
     gene            551..781
                     /locus_tag="PKJ16_10530"
     CDS             551..781
                     /locus_tag="PKJ16_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HNV47466.1"
                     /translation="MYLDRIELNPRVCGGKPVIRGTRIPVSVIISHLAEGESWDAVLA
                     GFPELTESDIRASLQYASASIEHTEFAETAAG"
     gene            785..1153
                     /locus_tag="PKJ16_10535"
     CDS             785..1153
                     /locus_tag="PKJ16_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HNV47467.1"
                     /translation="MRLYLDQMFSCTLADILRNDGHDIVRASDVGQSRADDERILEYA
                     IMEVRTLVTLDEHFGDWTVLPLKKHPGVIRVKIHPPATSAIAQRLLPFLRTHDQEQFA
                     NNLIILGKDRVRWINTARGE"
     gene            1396..3114
                     /locus_tag="PKJ16_10540"
     CDS             1396..3114
                     /locus_tag="PKJ16_10540"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47468.1"
                     /translation="MQWLRNSARVVFGARIGGVRIFPIATKIVLVYVLFILLSNFSSH
                     YISLMLYRSELVKQARQLLVRDLKDAYTFANTQYEIFHFSGNRDQALRRIEEKALLDF
                     KHRKSLLLGLARDGRIEINAARTAKPAAFTDAAALAAMRPGQAATENEGFISFSLGGE
                     KYFGVYKHNPSWDMLLVRAEEFGEFNEDSNRIFRTISVLIVVITIACAAIGVFIINYV
                     LRFVRLITADIMRMTREHRIGLIDMHGSQADDVTFMGMAFNSLAGIIGTMMNIFQKFT
                     NKDIVLKAYEEKRVRLEGSRRELTCLFSDIKSFTYMTEVLGTDIITLLNLHYTSAIGI
                     ILRNDGIIGSIIGDALLAVYGVFGEESARMKSYQAVVSGYKTHEVAAEIRAKMQRIRD
                     DIVEKNGKLTPLEEKVYKAVLIEVGVGIDGGHVFYGNIGSYERMTNTVIGDTVNSASR
                     LEGLNRVYHAPVICSEYVVNDIKANVPDHGLEFFELDLVKVKGKTEGKRVFWPIFKKD
                     ITPAMRADLRHYSAGLKLYYAGKWKQAHARFRQCSLPAAAVFLERTGHGTCPKNWKGI
                     WTMETK"
     gene            3111..4226
                     /locus_tag="PKJ16_10545"
     CDS             3111..4226
                     /locus_tag="PKJ16_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47469.1"
                     /translation="MKTPRGARSRPAARRGASRKTSADLPAEQPPFLREGIGAPPEEG
                     ETYDLEREFAKSRGNRSVPIHVAIVAFALALAAVSAWFIYDIQRGMDRTTVGIADFRD
                     INLQELLAALQRARQELGRMEDTIAVARQKAQLEIERIRHEAAREIRKVEQSGLGQAE
                     KRRIIERIRAEEDRKLRENRRVADGAIKQKAAEAAAARARLTAFEKRAAAEQAAAIKD
                     SDLKVRAAYRLGDEKEQLVQSKLREQRTEHQERIKDFERELKEVKAAVDKEAERASDT
                     DNLLALYRRALTYYAKSRGEHGFVIDPGSGGTMLVDVNPFLDLKAGDRAYVLNKDNKI
                     VALVEIRPAGRRVMAKVVKRMKSGAIQPFDKILLKKN"
     gene            4239..5363
                     /locus_tag="PKJ16_10550"
     CDS             4239..5363
                     /locus_tag="PKJ16_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47470.1"
                     /translation="MRNAIIILLAILTAPSLLLGAEWEEHGVTVVSTGPSVTEGAMRH
                     ALRDANGRSFAANYAAYPDKPSRERAVALKDLFFGWRHILPERLEFFFAADGTYANMQ
                     TTRIEWQGENLRPYLPAGLAFHDARGELYYRFRIVVKDTSYKLDGKYSDEETLLKEIY
                     GFIRAVEEGKISETDLVRYRYEEPKKEAPKKELPLRLSAFVLGNYLTPTGDFAKIFSG
                     GYGGMAGVTLHNVGISMHDKTLFRVDLSLAAGYWGLERKKDPDPEVTGEVRSSYVVPI
                     IFTARYRIDVIGALFVAPSLGVGIGYHSLCYSKIVANNRRETCEVGEWAPSLSAGLMA
                     GYSLFNDTLMLMGGADYIVLFERQMTCMMFAFYIGAGYVF"
     gene            5462..6235
                     /locus_tag="PKJ16_10555"
     CDS             5462..6235
                     /locus_tag="PKJ16_10555"
                     /inference="COORDINATES: protein motif:HMM:NF039835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNV47471.1"
                     /translation="MRAFGIIAVCMLACLPPACERNTNDPTVASANRHIVTFDSLGGS
                     AINQQAVAPGERLVEPAAPSRAGHTFTGWFTDPACSKPWNFKANTITGDSTLYARWSV
                     NRYTLRYAAGPNGSIGGPAMQAVSHGESGATVTAIPARGHHFVQWSDGSTANPRTDAA
                     VTANVSVTAAFLIKQYPLTYAPGAGGSIAGTALQVVNHGASGSPVTAVPNAGHRFAQW
                     SDGSTANPRTDAAVTGAISATAIFTAEKHRRGIAPPESR"
     gene            complement(6257..6550)
                     /locus_tag="PKJ16_10560"
     CDS             complement(6257..6550)
                     /locus_tag="PKJ16_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HNV47472.1"
                     /translation="MKQVLFHPVAEAEMAESALWYENKQKNLGKRFLEAVESALKRIQ
                     IQPELYRVLENDIRMCGVGRFPYGIIFREKERSIEVIAIMHLKRKPGFWKERV"
     gene            complement(6547..6777)
                     /locus_tag="PKJ16_10565"
     CDS             complement(6547..6777)
                     /locus_tag="PKJ16_10565"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HNV47473.1"
                     /translation="MTKKLEALIEDIMSLPGTSRAFIAEKILESLEYDEMGAVSDAWK
                     QEIRKRCRDIDEGAVALEEAEAVFSRSFKRIS"
     gene            complement(6935..7888)
                     /locus_tag="PKJ16_10570"
     CDS             complement(6935..7888)
                     /locus_tag="PKJ16_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47474.1"
                     /translation="MEKITHLNFSRYTPAELASTKGRQRLYVVISTLGEREADLAAEK
                     VRLVGDELAGMVDRVFLSHRREHADAPDRTEQRVRAADPNAVIIITNAEAVPDMGDER
                     GKGADMRRTLYHINTHWRDGLPPEDIVIVFLDADVSMQYFGAHFVTGLAGAVFEGYDF
                     AKAGFWRAMGRVKKYMAQPLFSAIAHPAVRRLTAFSYPLSGEVAGTLAFFNAVCFWQM
                     YGIETGVAIDSCFQGYRMADVNLGRYDHDHNSDLGIQKIAFGVVRTWLRQMVEYGVIE
                     LKDGAAVNDILQFNAIDENGERQFHSYDLTERKYAPLKEIV"
     gene            8023..8361
                     /locus_tag="PKJ16_10575"
     CDS             8023..8361
                     /locus_tag="PKJ16_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47475.1"
                     /translation="MKQLIKRHRIKVTEGQSNGLLETAASGLVRMLGVNDIDVDRKTG
                     TIDITYDLAHTSYRALERKLGELGCATASSPLQSIKRALINFTEQNQIVSRHLETVNP
                     EAARRMRARR"
     gene            complement(8379..9314)
                     /locus_tag="PKJ16_10580"
     CDS             complement(8379..9314)
                     /locus_tag="PKJ16_10580"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47476.1"
                     /translation="MAWNGMLSGQMPNKTEFDHFIEIDDVNLHYRHYPAKKKKSPHIV
                     LLHGFASSTYTWEKIDRRLNDEGFHIWALDMKGFGWSDKPRDSSYDAISLAEQVNRWM
                     EALDIEQAIFVGNSYGGGVAAMLAFSYPKRISKVIAIDPATGQRMKLPFILRLLRFPF
                     ARDIGKLIYAKWMIRLVFREVYYHREWITEEQIEEYYQRINTANAIEAQVSLAKQINF
                     DLYKAYTTRLHELTQPALLVWGANDKWIPTQLGIDLCATLPRARMVIIPECGHVPQEE
                     KPDLTFRLIMDFIRGKDPLKIYDGAIAGNVITLNP"
     gene            complement(9371..10282)
                     /locus_tag="PKJ16_10585"
     CDS             complement(9371..10282)
                     /locus_tag="PKJ16_10585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47477.1"
                     /translation="MIDKSPYDHFVEIDDITYHYREYPARGKNAEHVVLLHGFASSTY
                     SWEKIDYRLHEEGYHVWSLDLKGFGWSDKPRNGDYDILTIMEELNKWMVAMKIDDAIV
                     VANSYGGGVALLLSFNYPKRIKKMVLIDNAGLRMRLPSVMHLISLPLAGLVGKIIFGR
                     WMVKLIVREVFHDKRLITDEQIDAYHARMSTTNATDAQIALCRAIDFDKYELYYSRIR
                     EIKQKTLIVWGKNDAWIPLELGYRLNDMLPDARLVIIPECGHIPQEEKPETVFRLVLD
                     FARGRDVFATFRGTISGNVCAIDEQTA"
     gene            complement(10493..>10869)
                     /locus_tag="PKJ16_10590"
     CDS             complement(10493..>10869)
                     /locus_tag="PKJ16_10590"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV47478.1"
                     /translation="FRALVREHADIVFVNEDEAQAFTGESPERALEAIAAMCRIAVVK
                     IGAEGSLVKHDGAVHRIPSFKTNVANTNGAGDMYAAGLLYGITHGYSVDKAGLIGSYA
                     SSLVVASHGARLSEKLDVSSIV"
BASE COUNT         2150 a         3455 c         3294 g         1970 t
ORIGIN      
        1 tccctagcac ccaatcgtaa tattcatagg ggccctcgcc caatcgcctc gggcggcaat
       61 gcccaacctg gcgcgtacag acggggcact gccccgtctc aacaggaggc tgccacatgg
      121 caaccgtgaa attcacttcc atcatccagc acgcaagcgg gcaattcggc gggatcgtct
      181 tctcccaccg taaggggaag ccgtacctca agcgatacgc gaagccgcgc gacccgcgca
      241 cggcggcgca ggtcgcagcc cgtgagcgct ttcgggaatg cgccgcgaaa tggcgcacgc
      301 tcccgccgga ggagaaggcc gcgtggaagg cgcgcgcgcg gagagagaac gcgacggggt
      361 ataatctgtt catgcgggag gggatgcgcg atagggcggg tgcttaaaag gccgggcaat
      421 tccccgccag ccatgggcga atggggaatt tatacggatt gtctgttgat ttgcttgacg
      481 cacgtggaca tacgtgccag tctcaaatag tcgtggttcc tttaatccca gaataaggag
      541 aacgccccgc atgtatctcg acagaataga actcaatccc cgggtttgcg gcggcaagcc
      601 ggtcatcaga ggcacccgga ttccggtatc ggtgatcata tcgcacctgg ccgaaggcga
      661 aagctgggac gccgtactgg cgggattccc cgagcttacg gaaagcgaca tccgcgcatc
      721 cctccagtat gcctccgcct ccattgagca taccgaattt gccgaaacgg cggcaggata
      781 attcatgcgc ctgtacctcg accagatgtt cagctgcacg cttgcagaca tcctgcgtaa
      841 cgatggccac gatattgtgc gcgcttcaga cgtcggacag tcaagggcgg atgacgagcg
      901 gatccttgaa tacgccatca tggaagtgcg gacgctggta actctcgacg agcactttgg
      961 agactggacc gttttaccct tgaagaaaca ccccggtgtt atacgtgtta agatacatcc
     1021 accggcgaca agcgcaattg cccaacgcct cctcccattc cttagaacac acgatcagga
     1081 acaattcgcg aacaacctta taatccttgg aaaagaccgc gtgcgatgga ttaataccgc
     1141 gagaggggaa tagcatcccc atgcaatacg aacggggcca aatgtatcgt ttttccgccc
     1201 tggcccccgg ccgtttcgcc ccgttccacg cactgttcgc tccccgggac gcaccgttgg
     1261 ttccgcggtc cttgacgccg ggggagttcg tgcggtagta ttacaccagg agttgcttcc
     1321 gccgctccag cacgcacagc cgcagccggt tcgcgccggt cgtgaataca ttccgtctca
     1381 aaggggagtc ccgccgtgca atggttacgc aacagcgccc gtgtcgtctt cggcgcgagg
     1441 atcggcgggg tccgcatctt ccccatcgcc acgaagatcg tgcttgtgta cgtgctcttc
     1501 atcctgctgt ccaacttctc gtcgcactac atcagcctca tgctctaccg gagcgagctg
     1561 gtcaagcagg cgcggcagct cctggtgcgc gacctcaagg acgcctacac cttcgcaaac
     1621 acccagtacg agatattcca cttcagcggg aaccgcgacc aggcgctgcg ccgcatcgag
     1681 gagaaggccc tcctggattt caagcaccgc aagtccctgc tcctggggct cgcccgcgac
     1741 gggcgcatcg agatcaacgc cgcgcgcacc gcgaagcccg cggccttcac cgacgcggcg
     1801 gccctggcgg cgatgcggcc gggccaggcc gcgaccgaga acgagggctt catctcgttc
     1861 tccctcggcg gggaaaagta tttcggggtg tacaagcaca atccgagctg ggacatgctc
     1921 ctggtgcgcg cggaggagtt cggcgagttc aacgaggact ccaaccggat cttccgcacc
     1981 atctccgtcc tgatcgtcgt catcaccatc gcctgcgcgg ccatcggcgt cttcatcatc
     2041 aactacgtgc tacgcttcgt gcgcctcatc accgccgaca tcatgcggat gacgcgcgag
     2101 catcggatcg gcctcatcga catgcacggc agccaggccg acgacgtgac cttcatgggc
     2161 atggccttca actcgctggc cggcatcatc ggaaccatga tgaacatctt ccagaagttc
     2221 accaacaagg acatcgtgct caaggcctac gaggagaagc gcgtgcgcct cgaggggtcg
     2281 cggcgggagc tcacctgcct cttctcggac atcaagagct tcacctacat gaccgaggtg
     2341 ctgggcaccg acatcatcac cctgctcaac ctgcactata ccagcgccat cggcatcatc
     2401 ctcaggaacg acgggatcat cgggtccatc atcggcgacg cgctcctagc ggtgtacggc
     2461 gtcttcggcg aagagtcggc gcggatgaag tcgtaccagg ccgtggtgtc gggctacaag
     2521 acccacgagg tggccgccga gatccgcgcg aagatgcagc gcatccgcga cgatatcgtc
     2581 gagaaaaacg ggaagctcac cccgctcgag gagaaggtct acaaggccgt gctcatcgag
     2641 gtgggcgtgg gcatcgacgg cgggcacgtc ttctacggga acatcggctc ctacgagcgc
     2701 atgaccaata cggtgatcgg cgacacggtg aactccgcct cgcggctgga ggggctcaat
     2761 cgcgtgtacc acgccccggt catctgctcc gagtatgtcg tgaacgacat caaggcaaac
     2821 gtgccggacc acgggctcga gttcttcgag ctggacctcg tgaaggtgaa ggggaaaacc
     2881 gagggcaagc gggtcttctg gcccatcttc aagaaggaca tcacgcccgc catgcgcgcg
     2941 gacctgcgcc attactccgc cggcctcaag ctctactacg ccgggaagtg gaagcaggcg
     3001 catgcgcgct tccgccagtg ctcgctcccg gcggcggcgg tgttcctcga acggaccggg
     3061 cacggaacat gcccgaaaaa ctggaagggc atctggacga tggaaacgaa atgaaaacac
     3121 cacgcggcgc acgatcccgc cccgcggcgc ggcgaggggc gtcccgaaag acctccgcgg
     3181 acctgccggc cgaacagccc cccttcctgc gggaggggat cggcgcgccc cccgaagagg
     3241 gcgagaccta cgacctcgaa cgggagttcg cgaaaagccg gggcaaccgg agcgttccga
     3301 ttcacgtcgc gatcgtcgcc ttcgcgctgg ccctcgcggc cgtgagcgcg tggttcatct
     3361 acgacatcca gcgcggcatg gaccggacga ccgtcggcat cgccgacttc cgcgacatca
     3421 acctgcagga gctccttgcg gccctccagc gcgcccggca ggagctgggg agaatggagg
     3481 acaccatcgc ggtcgcccgg cagaaggcgc agctcgagat cgagcggatc cgccacgagg
     3541 ccgcgcgcga gatcaggaag gtggagcagt cggggctggg ccaggcggag aagcgccgga
     3601 tcatcgagcg cattcgcgcg gaggaggacc gaaagctccg ggagaaccgc cgcgtcgccg
     3661 atggcgccat caaacagaag gcggcggagg cggccgcggc gagggcgcgc ctgacggcct
     3721 tcgaaaagcg ggccgccgcg gaacaggccg ccgcgatcaa ggactcggac cttaaggtgc
     3781 gggcggccta tcgactcggc gacgagaagg aacagctcgt ccagtcgaag ctgcgggaac
     3841 agcgcacgga gcaccaggag cgcatcaagg acttcgagcg cgagctcaag gaggtcaagg
     3901 cggcggtcga caaggaggcg gagcgtgcct cggacacgga caatctcttg gccctctacc
     3961 ggcgggcgct cacctactac gcgaaatcgc gcggcgagca cggcttcgtg atcgatcccg
     4021 gctccggcgg aacgatgctc gtggacgtga acccgttcct cgacctcaag gcgggggacc
     4081 gggcctatgt gctcaacaag gacaacaaga tcgtggcgtt ggtggagatc cggcccgccg
     4141 ggcgccgcgt gatggcgaag gtcgtgaagc gcatgaagag cggcgcgatc cagcccttcg
     4201 ataaaatttt gctcaagaag aactgaccgg taggcacaat gagaaacgcc atcatcattc
     4261 tactcgcgat cctgaccgcc ccctcccttc tcctcggagc ggaatgggag gagcacggcg
     4321 tcacggtggt ctcgacggga ccctcggtta ccgagggcgc catgcgccat gcgctcaggg
     4381 acgcgaacgg ccggtccttc gccgccaact acgcggccta tccggacaag ccctcacgcg
     4441 agcgcgccgt cgcgctcaag gacctcttct tcggctggcg gcacattctc cccgagcgcc
     4501 tggagttctt tttcgctgcg gacggcacct acgccaacat gcaaaccacg cgcatcgaat
     4561 ggcagggcga gaacctgcgg ccctatcttc cggccggcct cgccttccac gacgcgcgcg
     4621 gcgagctgta ctaccgcttc cgcatcgtcg tcaaggacac gtcatacaag ctggacggaa
     4681 agtattccga cgaggagacg ctgctcaagg agatctacgg cttcatccgg gccgtggagg
     4741 aagggaagat ctccgagacg gacctggtgc ggtaccgcta cgaggagccg aagaaggagg
     4801 cgccgaaaaa ggagctgccc ctgcgtctct cggcgttcgt actcgggaat tacctaacgc
     4861 ccaccggcga tttcgcgaag attttctcgg gcggctacgg cggcatggcg ggcgtcaccc
     4921 tgcacaatgt gggcatatcg atgcacgaca agaccctctt ccgcgtggac ctctccctgg
     4981 cggccggcta ttgggggctc gaacggaaga aggacccgga ccccgaggtg accggcgagg
     5041 tgcgctcctc gtatgtcgtt cccatcattt ttacggcgcg ctatcgcatt gacgtgatcg
     5101 gcgctttgtt cgtcgcgccg tcgctgggtg tgggcatcgg ctaccacagc ctgtgctaca
     5161 gcaagattgt ggccaacaac cggcgtgaga cctgcgaggt gggcgaatgg gcgccgtcgc
     5221 tctcggcggg gttgatggcc ggctattcac tgttcaacga caccctcatg ctcatgggcg
     5281 gcgccgacta catcgtattg ttcgagcggc agatgacctg catgatgttt gccttttaca
     5341 taggagcggg atatgtgttt taaggccgga tataccggga caccggccgg cggcgttccc
     5401 tgctgggacg gggcgatgct tcgtcccggc ggggatcagc gttcgcatgg ggcgcgttga
     5461 tatgcgcgcg ttcggaataa tcgccgtctg catgctcgca tgtctcccgc ccgcctgcga
     5521 acgcaacacg aatgatccga cggtcgcatc ggcgaaccgt cacatcgtca ccttcgattc
     5581 cctgggcgga agcgcgatca atcaacaggc cgtcgcaccc ggcgagcggc tcgtcgagcc
     5641 tgcggcgccg tcgcgggccg ggcacacctt cacgggatgg ttcacggacc ccgcctgttc
     5701 caaaccctgg aatttcaagg ccaatacgat aacgggagac agcaccctct atgcgcgctg
     5761 gtcggtcaat cgctacaccc tgcgctacgc ggcgggcccc aacggcagca tcggcggccc
     5821 ggccatgcag gcggtctcac acggcgaaag cggcgccaca gtgaccgcca tccccgcgcg
     5881 cggccatcat ttcgtccagt ggagcgacgg ctccaccgca aatccgcgga ccgacgccgc
     5941 cgtgaccgcg aacgtgtcgg tcaccgcggc ctttcttata aaacagtatc cgctcaccta
     6001 tgcgccgggc gcgggcggct ccattgccgg gaccgccctg caggtggtga accacggcgc
     6061 ctcgggatcg ccggtgaccg ccgttcccaa cgccgggcac cgctttgcgc aatggagcga
     6121 cgggtccacc gccaatccgc gtacggacgc cgccgtgacc ggcgccatct ccgcaaccgc
     6181 catattcacc gcggaaaagc accggcgggg tattgccccg ccggaatcgc gatgaagtag
     6241 ttccgctcga actcgactaa actctctcct tccaaaaacc gggctttcgc ttgagatgca
     6301 ttatggcaat gacctcaatc gatcgttctt tttcacgaaa aatgattccg tacgggaacc
     6361 tgccaacacc gcacatgcgt atatcgttct caagcactcg gtacagctcc ggctggattt
     6421 gtatgcgttt cagcgccgat tcgaccgcct cgagaaaccg cttgccgaga tttttttgtt
     6481 tgttctcata ccatagtgcc gattcagcca tttccgcctc ggcgaccggg tgaaacagta
     6541 cctgtttcat gaaatacgct tgaatgatct cgaaaaaacc gcctcagcct cttctaatgc
     6601 aaccgcgcct tcatcaatat cacgacagcg tttccgtatc tcctgcttcc atgcatccga
     6661 cacggctccc atttcatcgt attcaaggct ttccagtatt ttctcggcaa taaacgcccg
     6721 tgatgttccc ggcaggctca taatatcttc aatgagggcc tcaagctttt tcgtcatccg
     6781 agttctccag aacagtgttt atcatacaac gatcattcac catgaataat acagataaga
     6841 aatgaacagg acaaccagaa taacttcatg agaagtaaaa tttacatccg tcagtaccgg
     6901 ctgcggataa accatcccca acgacgtcac gatactacac gatctcctta agcggcgcgt
     6961 acttccgctc ggtcaggtcg tagctgtgga actgccgctc gccgttttcg tcgatggcgt
     7021 tgaattgcaa tatatcgttc acggccgcgc cgtccttgag ctcaatcacg ccgtactcga
     7081 ccatctgccg cagccaggtg cgcaccacgc cgaaggcgat cttctggatc ccgagatcgg
     7141 aattgtggtc gtgatcgtag cggcccaggt ttacgtcggc catgcggtag ccctgaaagc
     7201 acgagtcgat cgccacgccg gtctcgatgc cgtacatctg ccagaagcac acggcgttga
     7261 agaacgcgag ggtcccggcc acctcgcccg agagcgggta cgagaaggcc gtgagccggc
     7321 gcaccgccgg gtgcgcgatg gccgagaaga gcggctgcgc catgtacttc ttgacccgcc
     7381 ccatcgcccg ccagaacccg gccttggcga aatcgtaccc ctcgaacacc gcgccggcca
     7441 gccccgtgac gaaatgcgcg ccgaaatact gcatcgacac gtcggcgtcc aggaaaacga
     7501 tcacgatgtc ctccgggggc agcccgtccc gccagtgcgt gttgatgtgg tacagcgtcc
     7561 gccgcatgtc ggcgcccttc ccgcgctcgt cgcccatgtc cggaacggcc tcggcattgg
     7621 tgatgatgat gaccgcgttc ggatcggcag cgcgcacgcg ctgctcggtg cggtcgggcg
     7681 cgtccgcgtg ctcacgccgg tgggagagaa acacgcggtc caccatcccg gcaagctcgt
     7741 cgcccacgag ccgcaccttc tcggccgcca ggtccgcttc gcgctcaccg agggtcgaga
     7801 tgaccacata gagccgctgc cgacccttcg tcgaggcaag ctcggccggg gtatatcgcg
     7861 agaagttgag gtgggtgatt ttttccatat acggctctta tgccgtgcta tggaggcgcc
     7921 ggcaaaatgt ccattctttt cgccgaaaaa aatagatatc ccagcaaaag tgttatagta
     7981 ttacaccgca atagggacaa ttgaaaccaa tgggatagcg atatgaagca actgataaaa
     8041 cggcaccgca taaaggtaac agagggacag tccaatggac tgctggagac ggccgcaagc
     8101 ggcctggttc ggatgctcgg ggtgaacgac atagacgtgg accgcaagac ggggaccatc
     8161 gacatcacct acgatctcgc gcacacgagc tatcgggcgc ttgagcgcaa actgggcgag
     8221 ctgggctgcg ccaccgcgag ctcgccgctg cagagcatca agcgcgcgct catcaacttc
     8281 accgagcaaa accagatcgt gagccggcac ctcgagacgg tcaaccccga ggccgcccgg
     8341 agaatgcgcg cgcgtcggta ggcgcgcgcc ggtacgcgct acggattgag ggtgatgacg
     8401 ttcccggcga tcgcgccatc gtagatcttc agcgggtcct tcccccggat gaagtccatg
     8461 atgagccgga aggtgagatc gggcttctcc tcctggggga cgtggccgca ttcggggatg
     8521 atcaccatcc gcgcccgcgg aagcgtcgcg cacaggtcga ttccgagttg cgtggggatc
     8581 cacttgtcgt ttgcgcccca caccaggagc gccggctgcg tgagctcgtg cagccgcgtg
     8641 gtataggcct tgtataaatc gaaattgatc tgcttggcca gcgagacctg cgcctcgatc
     8701 gcgttggcgg tgttgatgcg ctggtagtat tcctcgatct gttcctcggt gatccactcg
     8761 cggtgatagt acacctcgcg gaacaccaac cggatcatcc acttcgcata gatgagcttg
     8821 ccgatgtcgc gcgcgaaggg gaagcgcaac agccgcagga tgaacggcag cttcatccgc
     8881 tgcccggtcg ccgggtcgat cgcgatcacc ttcgagatgc gcttcggata gctaaaggcg
     8941 agcatcgcgg ccacgccgcc gccgtaggag ttccccacga aaatggcctg ctcgatatcg
     9001 agcgcctcca tccatctgtt cacctgctcc gccaggctga tggcgtcgta gctggaatcg
     9061 cgcggcttgt cggaccagcc gaagcccttc atgtccagcg cccatatgtg gaagccctca
     9121 tcattgaggc gccggtcgat cttctcccac gtgtaggtgg acgaggcgaa gccgtgcagc
     9181 agcacgatgt gcggcgattt cttcttcttc gccgggtagt ggcgatagtg caggttcacg
     9241 tcgtcgattt cgatgaagtg gtcgaactcg gtcttgttcg gcatctggcc ggagagcata
     9301 ccgttccacg ccatgtcgtt gctccgtttt gtcgatgatg atgcggccgc ccagagcggg
     9361 cggcgagcgc ctacgcggtc tgctcgtcga tggcgcacac gttgcccgaa atcgtcccgc
     9421 gaaaggtcgc gaagacgtcg cgcccgcgcg cgaaatcgag caccaggcgg aacacggtct
     9481 cgggcttctc ctcctgcggg atgtgcccgc actcggggat gatcaccagg cgcgcgtcgg
     9541 ggagcatgtc gttgaggcga taccccagct ccagcgggat ccacgcgtcg ttcttgcccc
     9601 atacgatgag cgtcttttgc ttgatctccc gaatccggga gtaatacagc tcgtacttgt
     9661 cgaaatcgat ggcgcggcac agcgcgatct gcgcgtcggt cgcgttggtt gtggacatgc
     9721 gcgcatggta ggcgtcgatc tgctcgtcgg tgataagccg tttgtcgtgg aacacctcgc
     9781 ggacgatgag cttcaccatc caccgcccga agatgatctt ccccacgagc ccggccagcg
     9841 gcaggctgat caggtgcatc accgacggca gccgcatgcg cagccccgca ttgtcgatga
     9901 ggaccatctt cttgatgcgc ttcgggtagt tgaagctcag caggagcgcc acgccgccgc
     9961 cgtaggagtt ggcgaccacg atggcgtcgt cgatcttcat cgccaccatc cacttgttga
    10021 gctcctccat gatcgtcagg atgtcgtagt cgccgtttct cggcttgtcg gaccagccga
    10081 agcccttgag gtccagcgac cagacatggt agccctcctc gtgcaggcgg taatcgatct
    10141 tctcccacga gtaggtggac gaggcgaagc cgtgcagcag caccacgtgc tcggcgttct
    10201 ttccgcgcgc ggggtattcc cggtagtgat aggtgatgtc gtcgatttcg acgaagtgat
    10261 cgtaggggct tttgtcgatc atctgccccg aagccatccc tttccacgac atattcatac
    10321 ctgcctcgat agtgtatcca gcggaatagc agccatttca accgtccggt caaactaaac
    10381 aggccgtgcg cgaatgtcaa gagcaaacat catgcgccac agggggcggt acagcggggg
    10441 ccgggccgat ccgcttcgcg gcggcggaac attcagcggt gcgccaacgc cattacacga
    10501 tgctgctcac atcgagcttt tcgctcaacc gcgcgccgtg gctggccaca acgagcgacg
    10561 aggcatagct gccaatgaga ccggccttgt ccacggaata gccgtgggtg atgccgtaga
    10621 gcaggcccgc ggcgtacatg tctcccgcgc cgttggtatt ggccacgtta gtcttgaacg
    10681 aggggatgcg gtgcaccgcg ccgtcgtgtt tcaccaggct cccctccgcg ccgatcttca
    10741 ccacggcgat acggcacatc gcggcgatgg cctccagcgc gcgctcgggg gactcgcccg
    10801 tgaaggcctg cgcctcgtcc tcgttcacga agacgatgtc ggcgtgctcg cgcaccagcg
    10861 cgcggaacg
//
LOCUS       DAORHF010000115        10835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5315_length_10835_cov_5.462245, whole genome shotgun sequence.
ACCESSION   DAORHF010000115 DAORHF010000000
VERSION     DAORHF010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5315_length_10835_cov_5.462245"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PKJ16_10595"
     CDS             complement(<1..628)
                     /locus_tag="PKJ16_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47479.1"
                     /translation="MRHKSVIYDTIIENTFLVIGLVCLFFLFLIMFFLFREGIPIFKT
                     VGVFDFILGDSWYPTSEVPRFGVFPLIVASIAVTLLASVIAVPFSLAIAIYLSELARP
                     TVDALVRAGLSQADAERRAALVLLLVTLPGGPPREVLAEALGTERGRVFLGVNESESI
                     TWLVKERFAELATATAEREVVLGRGSGALAVREAEDAMRLAEREGYRVD"
     gene            complement(765..1355)
                     /locus_tag="PKJ16_10600"
     CDS             complement(765..1355)
                     /locus_tag="PKJ16_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV47480.1"
                     /translation="MPRPRRICIEEATYHVFSRCIDRRNLMLNDSYKEIMLNTIEKTQ
                     EKYVFELVFYQILDNHIHLVIKTCKGEATISRIMQYIKARFAENYNRKNGRTGPFWNE
                     RFGDVIVESQPNPVFYLMWLLWYLAYNAVRKAKVDDPREYLYGSINYYLDGTYKGKLK
                     ISIHRYFMGLGNRCTDRLEKFITFEEMYRKRLWMSV"
     gene            1594..1884
                     /locus_tag="PKJ16_10605"
     CDS             1594..1884
                     /locus_tag="PKJ16_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47481.1"
                     /translation="MPEKKGQSPPPSRSLIADEGDISKQPSRFDEEKLSDPSGEEIGS
                     LRFLKLIREKRDDTLVEIRDALFAEIKTFRKGQPQGDDMTVVLLRYIGEGRE"
     gene            1881..2747
                     /locus_tag="PKJ16_10610"
     CDS             1881..2747
                     /locus_tag="PKJ16_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HNV47482.1"
                     /translation="MKNLDILAALVRNRNSRALNRSLVGFAMPDFDVTIAHRDIAAYA
                     KATGDANPAYAAPDGPVPPFFLSRLIFDNVKRLLIHRDLGMNLMRMVHGELDAAWHRS
                     LRAGDRVRVRVYIKEISDTAAGELLVLAGEGYMDGQRALEASLGFIMRKAGGSGRKHD
                     ADEAAAPKELFRREVRTWDGQEREYARVSGDTMPIHTSTLVAKLAGLPRTILHGVCQM
                     AMCTAAFADERLSGDIARLASVRGRFAGIVIPGWTLTLVGFASDEPNTVPFTVLDPKG
                     RAVIKNGLFRHR"
     gene            3179..4222
                     /locus_tag="PKJ16_10615"
     CDS             3179..4222
                     /locus_tag="PKJ16_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative urea ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV47483.1"
                     /translation="MKKALCTLVTLACLVSLFGGCAAQKEYPIAWSHYTGWEPWDYAD
                     AAGILKKWGDKYKIKIKLVRVNDYVESINLFTAKKYYGCAMTNMDALTIPALGGVDST
                     ALIIGDFSNGNDGIVTKNETMVMGLKGKKVKLVQLSVSHYLLSRALSMNGMTEKDLTL
                     VNTSDADIGSVFLSDPAGIAVTWNPILMNVAQSKGVNLIFDSSKIPGEIIDLMVVHSD
                     APDELKKALVGAWYETMAVMNGGAKAGEALAHMAKSAGTTVDGFKAQLATTAMFYKPG
                     EAAKFARNKKLATTMEYVRTFSFEKGLYGQGNKTKDFVGISFPDGSVLGDRNNVKLRF
                     DDRYMQMAADGKL"
     gene            4241..5071
                     /locus_tag="PKJ16_10620"
     CDS             4241..5071
                     /locus_tag="PKJ16_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HNV47484.1"
                     /translation="MTWRNPHFLGFAARPSKPLRVALGSALFVLCLALYLTASHLRHR
                     DNPQDKILPSFGQMATAVYQMGFQRDTRTGEYLMLVDTASSLRRLFIGVLLAAALGLV
                     LGLNMGLFPGVEALTAVFVTVVSIIPPLAVLPILFIAFGVDEFAKIMLILIGTFPLIC
                     RDIYLYTKKLPYEQVIKSLTLGAGAFTTAYRIVLPQVMPRLLEAMRLSFGAAWLFLIA
                     AEAIASTDGLGYRIFLMRRYLAMDVIIPYVFLITALGFAMDVSLKRFVLWRFRWYSGR
                     "
     gene            5082..5960
                     /locus_tag="PKJ16_10625"
     CDS             5082..5960
                     /locus_tag="PKJ16_10625"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV47485.1"
                     /translation="MSHHDRFLALENVYKAYDAKVVLDDIDLAVSRGEFCTLVGPSGC
                     GKSTLLRLLLGQEAPTAGAIMLDGAPLGYADTRRGVVYQKYSLFPHLTVLDNVLLGKR
                     ITAGALASRAVRSAMRDEAVEYLRRMGMADHAGKYPHELSGGMQQRVAIAQALIMKPQ
                     VLLMDEPFGALDPGTRENMQVFILELWEEAGMTVFFVTHDLEEAVFLGTRLLVLSQYY
                     TDGRGDGVQRGARIVADYQIKVASTRSKETADFGAFIAQIRREGFDPECRRHVADFNL
                     RHPDSFHTVSAAELQR"
     gene            6023..7696
                     /locus_tag="PKJ16_10630"
     CDS             6023..7696
                     /locus_tag="PKJ16_10630"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV47486.1"
                     /translation="MNRSMRNTLLAAGVLAALALAVTFCGKKEEAKDAVTPGEYASGK
                     IMRVPLIAWGADLVAINANGRSARTLKGSYFEREGINVELFREDNFMKQVDLFIKGDI
                     VYLRGTMGMMNMAVERLSASPATRPVLVFQHSWSAGGDALVVKPGIASVAQLKGKTVA
                     IQKNGPHVDYYLKLLKDAGLSFDEVKTVWTKDLVGPQGDTPMAKLARPDVDAAFVIIP
                     DALSLTSQGKVGTGAEDSVKGARILLSTKTASKIISDVYAVRKDYFDKNKEQVRKFVK
                     ALMASEEEVRDLFRTKSANEYKELLKIGGKLLLDDPNATTGVEGMYYDAEIAGYNGNV
                     RFFAEPNYPRNYQVLTREIQDSLIGAGIMTRRIDLLTAGWNYAELKAGLRYGDAVPGE
                     RFDPAKAVSVAERLGGDRTLFMFEIYFKPNQDAFPVAQYKREFDRVINFASTYGGALI
                     TVEGHADPMSFLRKQSEGAGEVVLKRIKQSAKNLSYSRAQAVRDQIVRYAKEKGIALD
                     ATQFTILGHGIAAPKFSVPKTSEEWQQNMRVQFRIMQVEAEEEVFRPLK"
     gene            7717..8448
                     /locus_tag="PKJ16_10635"
     CDS             7717..8448
                     /locus_tag="PKJ16_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV47487.1"
                     /translation="MMAYAIRSVCVAAVLCAMLASCAKREDPAASSGEVARPEAIRED
                     PWPPRLADVPVDLGQISRKNYIIIFDGSGSMTGHKIATAKAALKRFIMTILRDANVGL
                     VAFDRSGCTERASLGTPREKIVAIVDGITAGGYTPLGGAFTIAYDRLVHQANRQSGYG
                     EYHIVVLTDGRATDGRIMPQKVAAILAESPVVIHTIGFQIGSGHALNQPGRTYYTVAD
                     NFEELSRGLVAVLAELETFTVTTFK"
     gene            8450..10009
                     /locus_tag="PKJ16_10640"
     CDS             8450..10009
                     /locus_tag="PKJ16_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HNV47488.1"
                     /translation="MSAFSTSVSRIAGAVILAAVAGWGVYTFLLKSKEESVITYSTSD
                     ARNLKGEFTIALDSWIGYFPFTSPVFGQLMRDDGYRVKIIDDKADYPGRMKRLRAGDV
                     DFAVCTVDSYLLNGASQKFPGAIVAVIDESKGGDALVAWKDRIANIDELKRKAEYAIA
                     FTPASPSEHLLRAVRAHFDAPALLEKRHRVEVDGAEEAFKRLMAKKVDAAALWEPHVS
                     EALAAPGIVKLIGSDDMERLIVDILLVNRRFAENNPDMVKLFLKNYFETLRIYQAAPG
                     KLSENITGQLKLSAAQVAAMLRGVRWINYAGNAQWFGIGARETHRRPELVESIHATVG
                     ILTHSGDITSDPLPDRDPYTIINSAFLRDLPSFSPSLAAVRYEERGNTLIRKFSRLSQ
                     GDWNSLGTVGSLKLRPVSFRSGTEEIDESGREEVGQIVESIRHYPNFRILIKGHSGRR
                     GDPEANRELSLRRAKAVMDELVDTYHIDPNRIRIAGAGSAEPLPRQDGESDASYDARL
                     KRVEIVFLSKK"
     gene            10124..10771
                     /locus_tag="PKJ16_10645"
     CDS             10124..10771
                     /locus_tag="PKJ16_10645"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HNV47489.1"
                     /translation="MKLLKRLLPAIALGAAAFFAFFWLFEAGALVSLLFGVGGFFGGY
                     LLLAPRTIDPEFAELERVYGITPSMIKKTVAEGSTKVKKMRALLGKVDDADVRGTIGA
                     IIEHTEHIFLDLKKDPKDIKAARQFLAYYLDATINIIEKYIALSHMRSSGDREKTIGK
                     VRDLLQSVEAAFRKQLDQLYNDDFLDLDAEITVLENVIRQDGLADPKRTSAGGTK"
BASE COUNT         2078 a         3308 c         3467 g         1982 t
ORIGIN      
        1 gatcgacgcg gtagccttcg cgctcggcga ggcgcatcgc gtcctcggcc tcgcgcaccg
       61 cgagcgcgcc gctgccgcgg ccgagcacga cctcgcgctc ggcggtcgcg gtcgcgagct
      121 ccgcgaagcg ctccttcacg agccacgtga tgctctccga ctcgttcacg ccgaggaaca
      181 cgcgtccgcg ctcggtcccg agcgcctcgg cgaggacctc gcgcggcggg ccgccgggca
      241 gcgtgaccag gagcaggacg agcgcggcgc ggcgctcggc gtccgcctgg gagaggccgg
      301 cgcggacgag cgcgtcgacg gtggggcggg cgagctccga gagatagatc gcaatggcca
      361 ggctgaacgg caccgcgatc acggaggcca gcagcgtgac ggcgatcgac gcgacgatga
      421 gcgggaacac gccgaagcgc ggcacctcgc tcgtgggata ccagctgtcg ccaagaatga
      481 aatcgaatac gccaacggtc ttgaagatcg ggattccctc ccggaacaag aagaacatta
      541 taaggaaaag gaagaagagg cacacgaggc cgatcaccag gaaggtgttt tcgatgattg
      601 tgtcgtagat gacgctttta tggcgcatga tgtagtccgt gacggaacat tgggatgtag
      661 ggaatgtccg gcggtgcggt taagattttg ttaagaaaat atcaataatc cgcaaatgtg
      721 taagccgctt gactgcgggc tctgaccctc cttctcccat gtcttcatac ggacatccag
      781 agtcttttac ggtacatctc ctcgaatgta ataaacttct ccagtcgatc ggtacatcga
      841 ttcccgagtc ccatgaagta tcgatgtatt gaaatcttca gttttccctt gtatgtgcca
      901 tcgagatagt aattgatgct tccgtagaga tactctcttg gatcgtcgac cttggctttt
      961 cgaaccgcgt tgtaggcaag ataccacagc agccacataa gatagaaaac cgggttgggt
     1021 tgggactcga cgatgacatc gccaaatcgc tcattccaaa acgggccagt tctgccgttc
     1081 ttcctattgt agttttccgc gaaacgggct ttaatgtatt gcatgatgcg ggatatcgtc
     1141 gcctcgcctt tgcaggtttt aatgaccaaa tgtatatgat tatcgagtat ttggtaaaat
     1201 acgagttcga aaacgtattt ttcctgagtt ttttcaatgg tgttgagcat gatctccttg
     1261 taggagtcgt tcagcatgag atttcggcgg tcgatgcagc gtgaaaaaac gtgataggtt
     1321 gcttcttcga tacagatcct gcgtgggcgt ggcatggctg tttcctcgct tggttcgtgg
     1381 tttaatgatg tcattaataa tacggacgta tgacatggag tttaaatttt tacggaaaaa
     1441 ataattgggg tcagagcctt catcgagcct tcatctgttc agagaatgat gctctgaccc
     1501 caattattat tcccttaatg gctctgaccc tacgagggac cctgcgaggc tgaaccggag
     1561 ggtaatcagt ccactacttc aatcagcact catatgccgg agaagaaggg tcagagcccc
     1621 cccccgtccc gcagcctcat cgcagacgaa ggtgacatca gcaaacaacc atcccgcttc
     1681 gacgaagaaa agcttagcga cccgtcaggc gaggagatcg gctcgctgcg ctttttgaag
     1741 ctcatcaggg aaaaaaggga tgacacgctc gtcgagatac gggacgctct gttcgccgag
     1801 atcaagacgt ttcggaaggg acagccgcag ggggacgaca tgaccgtggt tctgttgcgc
     1861 tatatcgggg aggggcggga atgaagaacc tggacatact ggcggcgctc gtgcgcaacc
     1921 gcaacagccg agcgctcaac cggtcgcttg tcggattcgc gatgccggat ttcgacgtga
     1981 ccatcgcgca tcgggacatc gccgcctacg cgaaggcgac gggcgacgcc aatcccgcct
     2041 atgccgcgcc cgacggcccc gtgccgccct tcttcctgtc gcgcctcatc ttcgataacg
     2101 tgaaacgatt gctcatacac cgcgatctgg gaatgaacct catgcgcatg gtgcacggcg
     2161 agctcgacgc cgcctggcac cgttcgctgc gcgcggggga ccgggtgcgc gtccgcgtct
     2221 acatcaagga aatcagcgac acggccgcgg gcgagctcct ggtgcttgcc ggcgaggggt
     2281 acatggacgg ccagcgcgcg ctcgaggcgt cgctcggttt tatcatgcgc aaggccggcg
     2341 gctcgggacg gaagcacgat gccgacgagg ccgccgcgcc gaaggagctc ttccgtcgcg
     2401 aggtccgcac ttgggacggg caggagcgcg aatacgcgcg cgtctcgggc gacaccatgc
     2461 ccattcacac gagcacgctg gtggcgaaac tcgccgggct cccgcgcacc atcctccacg
     2521 gcgtgtgcca gatggcgatg tgtaccgccg ccttcgccga cgagcgcctc tccggcgata
     2581 tcgcgcgcct agcgtcggtg cgcggccgct ttgccgggat cgtcatcccg gggtggacgc
     2641 tcacgctggt cggattcgca tccgacgagc ccaacacggt tcccttcacg gtgctcgacc
     2701 cgaaggggcg cgcggtcatc aagaacggtt tgtttcgcca ccgctagcga ttcgttccgg
     2761 tgcgggcgta ctggcgcgca taatgcgccg gcgtacggga tgtcgcatat cgcgcatcgc
     2821 cgtttcacgc gacagcggcg cagtatgatc cgcacttcgt gcgcggtcgg atcatgcctg
     2881 ttccgttgtt tatctgtatg ataaggcggc gcactcgaca atatcgcgcg agttgcgagg
     2941 cgatcgttgc gatactgccg gcagtacgtg aatatgggac cgtgcatacg catattttgc
     3001 gatgagcgcc tcgggtcgct gcaccgttat cgtcccatgc cgtaatggga aatcacgcat
     3061 cgcggcagat ccattcgagt ttcccgccgc aaaaattact tgcaatattc aaacccgctg
     3121 ttattctacc gaccacaatt ccaacggcac acatacaatc gccaatcggg agaaagccat
     3181 gaaaaaagca ctctgcacgc tcgtgacgct cgcatgcctc gtttcgctct tcggcggctg
     3241 cgcggcccag aaggagtatc cgatcgcctg gtcgcactac accggatggg agccgtggga
     3301 ctatgccgat gccgcgggca tcctcaagaa gtggggcgac aagtacaaga tcaagatcaa
     3361 gctcgtccgc gtgaacgatt acgtggagtc gatcaatctc ttcaccgcga aaaaatacta
     3421 cgggtgcgcc atgacgaaca tggacgcgct caccatcccg gcgctgggcg gcgtggactc
     3481 cacggccctc atcatcggcg atttctccaa cggcaacgac ggcatcgtca cgaagaacga
     3541 gacgatggtg atggggctca aggggaaaaa agtgaagctg gtccagctct cggtgtcgca
     3601 ctacctactc tcgcgcgcgc tgtcgatgaa cggcatgacc gagaaggacc tgacgctggt
     3661 gaacaccagc gacgccgata tcggctccgt gttcctctcg gaccccgcgg gcattgccgt
     3721 tacctggaat cccattctca tgaacgtcgc tcagtcgaag ggcgtgaacc tcatcttcga
     3781 ctcgtcgaaa attccgggcg agatcatcga cctgatggtg gtgcactccg acgcgccgga
     3841 tgagctcaag aaggcgctcg tcggcgcgtg gtacgagacc atggcggtga tgaacggcgg
     3901 cgcgaaggcc ggcgaggcgc tcgcgcacat ggcgaagtcg gcgggaacga cggtcgacgg
     3961 gttcaaggcg cagctcgcga caaccgcgat gttctacaag cccggcgagg cggcgaagtt
     4021 cgcgcgcaac aaaaagctcg cgaccaccat ggaatacgtc cgcaccttct ccttcgagaa
     4081 aggcctgtac gggcagggga acaagacgaa agacttcgtg ggcatcagct ttcccgacgg
     4141 ctcggtgctg ggcgacagga acaatgtcaa gctccgcttc gacgaccgct atatgcagat
     4201 ggcggccgac gggaagctgt agccggctcg tgtattccgc atgacctgga ggaacccgca
     4261 cttcctgggt tttgccgcgc ggccatcgaa gccgctgcgg gtggcgttgg gatcggcgct
     4321 gttcgtgctg tgcctggcgc tgtatctcac ggcctcgcac ctgcggcacc gggataatcc
     4381 gcaggacaag atcctcccgt cgttcggcca gatggcgacg gccgtctacc agatggggtt
     4441 tcagcgcgac acgaggaccg gcgagtatct catgctcgtg gacacggcat cgagcctcag
     4501 gcggctgttc atcggcgtgc tcctggccgc ggcgttgggg cttgtcctgg gtctcaatat
     4561 ggggctcttt cccggcgtgg aggcgctcac cgcggtcttc gtcaccgtgg tgtccatcat
     4621 cccgccgctt gcggtgctgc ccattctctt tatcgccttc ggggtggacg agttcgcaaa
     4681 aatcatgctc atcctcatcg gcacctttcc gctcatctgc cgcgacatct atctgtatac
     4741 gaagaagctg ccctatgagc aggtgatcaa gtcgctcacc ctcggggcgg gggcgttcac
     4801 gaccgcctac cggatcgtgc tgccccaggt gatgccgcgg ctgctcgagg ccatgcgcct
     4861 ctccttcggc gccgcgtggc tcttcctcat cgccgccgag gcgatcgcct cgaccgacgg
     4921 gctgggctac cggatcttcc tcatgcggcg ctatctggcg atggacgtca tcatcccgta
     4981 tgtatttctc ataacggcgc tgggttttgc gatggacgtg tcgctcaaac gcttcgtgct
     5041 ctggaggttc cgatggtatt cgggacgata ggcggccgcg catgagccat cacgatcgct
     5101 ttctggcgct cgagaacgtc tacaaggcct acgacgcgaa ggtggtgctg gacgatatcg
     5161 acctcgccgt gagccgcggc gagttttgca cgctcgtggg ccccagcggc tgcgggaaat
     5221 cgacgctcct gcggctgctc ctcggccagg aggcgcccac cgccggcgcc atcatgctgg
     5281 acggcgcgcc gctcggatac gccgacaccc ggcgcggggt ggtgtaccag aagtattcgc
     5341 tcttcccgca cctcacggtg ctggacaatg tgctcttggg caagcggatc accgcgggcg
     5401 cgctcgcgtc gcgggccgtg cgaagcgcca tgcgcgacga ggccgtggag tatctgcgcc
     5461 gcatgggcat ggccgatcac gccggaaagt atccgcacga gctctccggc ggcatgcagc
     5521 agcgcgtcgc gatcgcgcag gcgctcatca tgaagccgca ggtgctgctc atggacgagc
     5581 ccttcggcgc gctggacccc ggcacgcgcg agaacatgca ggtgttcatt ctcgagctct
     5641 gggaggaggc ggggatgacg gtctttttcg tgacgcacga tctcgaggag gcggtgtttt
     5701 tgggcacgcg ccttctggtg ctgtcgcaat actacaccga cggccgtggc gatggcgtgc
     5761 aacgcggggc gcgcatcgtg gccgactacc agatcaaggt cgcctccacc cgctccaagg
     5821 agacggccga cttcggggcg tttatcgcgc agatccgccg cgagggcttc gatcccgaat
     5881 gccggcggca tgttgcggac ttcaacctcc ggcatccgga ttcgtttcat acggtatccg
     5941 ccgccgagct gcagcggtga gcggcgcggc ggatggcgcc cgcgggggcg cgatcgacat
     6001 cagtgagcaa ggaaggaatc gaatgaaccg atcaatgcga aacacgctgc tggccgcggg
     6061 cgtacttgcg gcgctcgcgc tggccgtcac tttctgcggg aaaaaggagg aggcgaagga
     6121 cgcggtgacg cccggcgaat acgcctcggg gaagatcatg cgcgtgccgc tcatcgcctg
     6181 gggcgccgac ctggtggcca tcaacgcgaa cggtcggagc gcgcgcacgc tcaaggggtc
     6241 gtacttcgag cgggagggga tcaacgtgga gctgttccgc gaggacaact tcatgaagca
     6301 ggtcgatctc ttcatcaagg gcgacatcgt gtacctacgc gggacgatgg ggatgatgaa
     6361 catggccgtc gaacgcctca gcgcctcgcc cgcgacgcgc cccgttctcg tgttccagca
     6421 ttcgtggtcc gccggcggcg acgcgctggt ggtgaagccg ggcatcgcct cggtcgcgca
     6481 gctcaagggc aagaccgtcg cgatccagaa gaacggcccg cacgtcgatt attacctgaa
     6541 gctcctcaag gacgccgggc tctccttcga cgaggtgaag accgtgtgga ccaaggacct
     6601 cgtgggcccg cagggcgaca cgcccatggc gaagctcgcg cggcccgacg ttgacgccgc
     6661 cttcgtgatc atccccgacg cgctctcgct cacctcgcag ggcaaggtgg gcaccggcgc
     6721 ggaggactcg gtgaaggggg cgcggatcct cctgtccacg aagacggcga gcaagatcat
     6781 cagcgacgtg tacgccgtgc gcaaggacta cttcgacaag aacaaggagc aggtgcgtaa
     6841 gttcgtgaag gcgctcatgg cctcggagga ggaggtgcgc gatctcttcc gaacgaaaag
     6901 cgcgaacgaa tataaggagc tcttgaagat cgggggcaag ctccttctgg acgacccgaa
     6961 cgcgacgacc ggcgtcgaag gaatgtacta cgacgcggag atcgccggct acaacgggaa
     7021 cgtgcggttt ttcgcggagc ccaactatcc gcgcaactac caggtgctca cgcgcgagat
     7081 ccaggactcg ctcatcggcg cggggatcat gacccgacgc atcgacctcc tgaccgcggg
     7141 atggaactat gccgagctca aggcgggcct acgctacggc gacgccgtcc ccggcgagcg
     7201 gtttgatccg gcgaaggcgg tgtccgtcgc cgagcggctc ggcggcgacc ggacgctgtt
     7261 catgttcgag atctacttca agccgaacca ggacgccttc ccggtggcgc agtacaagcg
     7321 cgagttcgac cgcgtgatca atttcgcctc cacctacggc ggggcgctca tcaccgtgga
     7381 ggggcacgcc gatcccatga gcttcctgcg caagcagagc gagggcgcgg gcgaggtggt
     7441 gctcaagcgg atcaagcagt cggcgaagaa cctgagctac agccgggcgc aggcggtgcg
     7501 cgaccagatc gtccgatacg cgaaggagaa ggggatcgcg ctcgacgcga cgcagttcac
     7561 catcctgggc cacggcatcg ccgcgcccaa gttcagcgtc cccaagacga gcgaggaatg
     7621 gcagcagaac atgcgcgtgc aattcagaat catgcaggtc gaggcggagg aagaggtctt
     7681 ccgcccgctc aaataggtgc cggtatgcga aatgatatga tggcgtacgc gatccggtcc
     7741 gtctgcgtgg cggccgtact gtgcgcgatg ctcgcctcct gcgcgaagcg ggaggatccg
     7801 gccgcatcgt ccggggaggt cgcgcgcccg gaagcgatcc gggaggaccc gtggccgccg
     7861 cggctcgcgg acgttccggt cgacctcggg cagatatccc gcaagaatta catcatcatc
     7921 ttcgacgggt ccggcagcat gaccggccac aagatcgcca cggcaaaggc cgcgctcaag
     7981 cggttcatca tgacgatcct gcgcgacgcc aacgtgggac tggtcgcctt cgaccggagc
     8041 ggctgcaccg agcgcgcgtc gctggggacg ccccgggaga agatcgtcgc gatcgtcgac
     8101 gggatcaccg cgggcgggta tacgccgctg ggcggcgcct tcacgatcgc ctacgaccgg
     8161 ctggtccatc aggcgaatcg ccagtccggg tacggcgagt accacatcgt cgtgctcacc
     8221 gacggacgcg cgaccgacgg gaggatcatg ccgcagaagg tggcggccat cctggccgag
     8281 agcccggtgg tcatccacac gatcggtttc cagatcgggt ccgggcacgc gctcaaccag
     8341 ccggggcgga cctactatac cgtcgcggac aatttcgagg agctgtcccg ggggctcgtc
     8401 gccgttttgg cggagctcga gaccttcacc gtgacgacgt ttaaatgaca tgagcgcctt
     8461 ctcgacatcg gtcagtcgta tcgccggggc ggtcatcctc gccgccgtgg ccgggtgggg
     8521 cgtgtacacc tttcttctga aatcgaagga agagagcgtc attacctaca gcaccagcga
     8581 tgcgcgcaat ctcaagggcg agttcaccat cgcgctggac agctggatcg gctatttccc
     8641 gttcacctcg cccgtcttcg ggcagctcat gcgcgacgac ggataccgcg tcaagatcat
     8701 cgacgacaag gccgattacc cgggacggat gaagcgcctc cgcgcgggcg atgtcgattt
     8761 cgccgtgtgc acggtcgatt cctatctgct caacggcgca tcgcagaagt tccccggcgc
     8821 gatcgtggcg gtcatcgacg agtccaaggg cggcgacgcc ctggtcgcgt ggaaggaccg
     8881 catcgcgaac atcgacgagc tcaagcggaa ggccgagtac gccatcgcct tcacgccggc
     8941 ctccccgagc gagcacctgc ttcgggccgt gcgcgcgcat ttcgacgcgc cggcgctcct
     9001 tgagaagcgg caccgcgtgg aggtggacgg cgcggaggag gcgttcaagc gcctgatggc
     9061 aaaaaaggtg gacgccgccg cgctctggga gccgcacgtg agcgaggcgc tggccgcgcc
     9121 cgggatcgtg aagctcatcg gctcggacga catggagcgg ctcatcgtgg acattcttct
     9181 ggtgaaccgc cgcttcgccg agaacaatcc ggacatggtg aagctttttc tgaagaacta
     9241 cttcgagacg ctgcggatct atcaggccgc gccgggcaag ctttcggaga acatcaccgg
     9301 gcagctcaag ctttccgccg cgcaggtggc ggccatgctt cgcggggtgc gctggatcaa
     9361 ctacgcgggc aatgcgcagt ggttcggaat cggggcgcgc gagacgcacc ggcgcccgga
     9421 gctggttgag tcgatccacg cgacggtggg catcctcacg cacagcggcg acatcacgtc
     9481 cgatccgctt ccggaccgcg atccgtacac catcatcaat tccgcctttc tccgcgacct
     9541 gccgtcgttc tccccgtcgc tcgccgcggt gcgctacgag gagcgcggga acacgctcat
     9601 tcggaaattc agccgcctct cgcagggcga ctggaactcg ctgggcaccg tgggttcgct
     9661 caagctccgg ccggtgtcct ttcgcagcgg caccgaggag atcgacgaaa gcggccggga
     9721 ggaggtgggg cagatcgtcg agagcatccg gcactatccc aactttcgca tcctcatcaa
     9781 ggggcattcg gggcgccggg gcgatcccga ggccaaccgg gagctttcac tgagacgcgc
     9841 gaaggcggtg atggacgagc tggtggacac ctatcacatc gaccccaacc ggattcgcat
     9901 cgccggcgcg ggatccgcag agccgctgcc gcggcaggac ggcgaaagcg atgcgagcta
     9961 tgacgcccgt cttaagcgcg tggagatcgt gttcctgtcg aagaagtagg tgcgggtggc
    10021 gcggcggtgt ttggttttat gattgctccg tcgcgattgc gcctcgtatt tacgtgaaac
    10081 cctgtttcgt gccatctcaa gcggttgtat cattggaacc atgatgaaac tactgaaacg
    10141 acttctcccc gcaatcgcgc tcggcgccgc cgccttcttc gcctttttct ggctgttcga
    10201 ggcgggcgcg ctggtgtcgc tcctctttgg cgtcggcggt tttttcggcg gctatctgtt
    10261 gttggcgccg cgcaccatcg atcccgagtt cgccgagctc gagcgcgtct acggcatcac
    10321 cccctcgatg atcaagaaga ccgtcgcgga ggggagcaca aaggtcaaga agatgcgcgc
    10381 cctgctcggg aaggtggacg acgccgatgt ccgggggacg atcggcgcga tcatcgagca
    10441 caccgagcac atcttcctgg acctcaagaa ggaccccaag gacatcaagg cggcgcggca
    10501 gtttctcgcg tattacctcg acgcgaccat caacatcatc gagaagtaca tcgccctctc
    10561 gcacatgcgc tccagcggcg atcgcgagaa gaccatcggc aaggtgcgcg atcttctgca
    10621 gtcggtcgag gcggccttcc gcaagcagct cgaccagctc tataacgacg acttcctcga
    10681 tctcgacgcg gagatcaccg tcctggagaa cgtgatccgg caggacgggc tcgccgatcc
    10741 gaagcggacg tcggcgggcg ggacgaaata gggagcatcg acattgggtg cgcgacatcg
    10801 accgggacct gtcatatgaa cacgaccgag aattc
//
LOCUS       DAORHF010000116        10720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5411_length_10720_cov_4.912049, whole genome shotgun sequence.
ACCESSION   DAORHF010000116 DAORHF010000000
VERSION     DAORHF010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10720
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5411_length_10720_cov_4.912049"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="PKJ16_10650"
     CDS             complement(<1..702)
                     /locus_tag="PKJ16_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47490.1"
                     /translation="MMVVAGVMVRSNRANAANLNVYSNDLTYVVNSGYVVPGGFFGKY
                     FDNAIAAGLSLYYLPPEYFFATLFGWQFFFFEGTFGFSSMQLKENPETKMVFYTADAG
                     PVLYYPLFDWLYPSIGVSAGGYYSTLKLSSIEDSKTHADYLARASLGVIIPISSSFSL
                     RVYGQYSYYHMFGEQYTAISYNLAAGYNLHGGYGSQIPRDASPVIVQDLKFDDIFAVR
                     YELYNKKGIGSLTLKN"
     gene            complement(776..1489)
                     /locus_tag="PKJ16_10655"
     CDS             complement(776..1489)
                     /locus_tag="PKJ16_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47491.1"
                     /translation="MGSATTTIDENSTVPFADVIKRSNRQGAETQKHGFSGIIKKVFN
                     ANTRINTVTRVIAVRADPVHDHTRWMADGSSDAPPVDEDAIINRIKKEYEGGRYQRAL
                     QLYRTEAGAVKKKRDVVEYLAGISFLRMCDYREASRTFARIADGSGDPRLRSEAMFNA
                     GFAFFCINDYTASNKYLARYVRESSDDDTLPNAYLLHARNCIKLRDAERAKKDLMIIK
                     DRFRGSEAYPEAIKLLEQL"
     gene            1909..2817
                     /locus_tag="PKJ16_10660"
     CDS             1909..2817
                     /locus_tag="PKJ16_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HNV47492.1"
                     /translation="MFQSVDQAIASLEKEKYICSKEIATTVYLAMKMEKPILVEGPAG
                     VGKTELGKVIASSLGMDLLRLQCYEGLDEAKALYEWEYAKQLLYTQILKDMLGSMLSN
                     EKTLADAVKKLSGEEDVFFSDKFLLPRPILMSIMSKKPTLLLIDEIDKSDSEFEAFLL
                     EFLSDFQVSIPEIGTVRAAIKPFVLLTSNNAREMSDALKRRCLHLYIDYPSQELEEKI
                     VTLKVPECSSHLVRELVKSVHAIRALQLKKEPSISETLDWAKALAILGRDVLDAETAM
                     DTLNLILKYEKDIELVKKNAGKVFVH"
     gene            2824..4194
                     /locus_tag="PKJ16_10665"
     CDS             2824..4194
                     /locus_tag="PKJ16_10665"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HNV47493.1"
                     /translation="MISAYVSHKKINKKFYDFIDILKDAGVPVSTAETLDLFTALGHV
                     GFDDRHVFRQTLRTSLVKDYTDIPVFDRCFDAFFGKKGPAKGSDLPSAVAGAMEMESL
                     RDEGLSEQDLDSLTALLDDFIESLDDAIFFERTYQEILTLFLEDPVVASSSGALGMII
                     FNTRSRGQHGPMGDSGQRPDETINLNQVMLRQISRRVDERKIGTQIKDREQYLLNKFI
                     YQLKPDEVKEMKELVRRFGQKLKNRISLRKKRVKHGGIDVKRTIRKNLQFGGTPFKIY
                     HKDRKIDRPQLVVLCDISGSVNQYSRFMLLLTYTLQSLFSKVRTFAFISNMVEITPLF
                     MEMNPDTALNSIFGDTDFTYGWGSNYGRCFNMFVREYSDSLTSKTTVLVLGDARNNNQ
                     DPGLESFIKITERTRNVFWLNPEKRHLWDWNDSIASIYREQCTEMREVNSFIDLSEFI
                     DKLFTR"
     gene            4345..4668
                     /locus_tag="PKJ16_10670"
     CDS             4345..4668
                     /locus_tag="PKJ16_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47494.1"
                     /translation="MEIEFRDMGEHKIIQVNGEVDLYNVSELKKALFSVTDGTSRSVI
                     VDMKNVNYMDSSGIGALVAGQKKMKAHNGRFGLLNIHEDVLNILKLATLDRFFKIYEG
                     EDELI"
     gene            complement(4681..6276)
                     /locus_tag="PKJ16_10675"
     CDS             complement(4681..6276)
                     /locus_tag="PKJ16_10675"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47495.1"
                     /translation="MKKNGADPDAARPAKPVKHKAGTASSDADAAVTSGVKFSLRIKF
                     AIAIISLVVLIIITMSIYVIWHESGVLEKEILTLARREIDHMQTTAADALSTESELQL
                     LAITEDMKGLESIKYAYILDADNRIRQNLIPEKNGTVLDDDITRALKNYTELREPLRH
                     TLPDPEGGGLIYDFSKPLVEKLGKKRIGSVRLGFSDGIIRKEIRSMAQNIILIALLFI
                     GVSVVGAVALASFIIKPIRQLSEGAAIIGTGKLDYKITVSSSDEIGQLANEFNAMTEH
                     LRKARDIEIEQRIMQEQLDLAKEIQEGLNPMAFYDKTGVQIKGYTKAAKGVGGDYFDY
                     VDIDEHRVGALLSDVSGKGIPASLVMVMIRTVFVTYIHRKDISTASVVRAINDSLSAD
                     FAIDKFATLFFLIYDRRTRELTFTNAGHGPLFCYRASMGACTITKLDGMPIGITEDVD
                     YQQARVKLNPGDIVVMYSDGVTEMRNEKRDEYGRARLQKLIIDNNERNANDIVEAIVA
                     DVNDFRGNASPHDDMTTLVLKVV"
     gene            complement(6291..8216)
                     /locus_tag="PKJ16_10680"
     CDS             complement(6291..8216)
                     /locus_tag="PKJ16_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47496.1"
                     /translation="MRGIIRTTVAWTLAVTVFLSAAGPALAQTISEMERRAQEYYDNK
                     EFTRAINEWMRILELDPENDRVQRRVEYAYEEKQRKDFAFWMSRKHLRLARKTLLKNF
                     RVGKSNARVAVNNFVIAYRIDPRDPPVQVLREEIRRLDDDIRLAEAQQKLSEELKRRY
                     AQILEMAEQAMDARQYELALKHWNDILEFFADDPVALEGKRRAELAISNRIRYEQIMG
                     LINSGKEMMKNEKYSEAKLEFEAVLRIDPKNRQARNLIEELDDRLENRKNFELRRLQA
                     EKFYVSGIANLNAKNFSQAEDDLENVLALIDNYKDTKERLASIKRLKAEYAEQLKRLR
                     LRQVDEKFQSGLVKLSASRYRDAVADFEEVIRMDPGNKLARDYIKTAQEALKQLEEEV
                     VDENSPYYSIVNSLVVSGKLLYDKADFAESKKSWEKILTLFPKNRIAIEYKLRCDLRL
                     NPKSYERFSSEIVDEGRQLLRAKNFQLALNKFQLIRSLSPDYPDIDRLIRQAEDGLAE
                     KKQVAGATPGEIESRYQLGLDLYRRGGRDNFVKALEQFRWIVARDPNNSRALISVNRI
                     ESQLRIGTTEDQRPESRLTDKQMQMVRYHYFRGINFYSNNNLNRAIEEWRKVLAIDPA
                     NAKARNNIQRALALMKK"
     gene            complement(8244..8693)
                     /locus_tag="PKJ16_10685"
     CDS             complement(8244..8693)
                     /locus_tag="PKJ16_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47497.1"
                     /translation="MKRTCMLIAAIALLATAVSCKKAPSTEEPSAILNNWAAAIKDLK
                     YDGYRKYEAYPRDEAVFAEMFRNNYFMDVMVNEVSEVDEKDVRKDQHGNRFIRKYVNF
                     EATSVKRATAKPDQLLRGDVVLIKFLDGKRAGDGWLISNRTMMYINR"
     gene            8831..>10720
                     /locus_tag="PKJ16_10690"
     CDS             8831..>10720
                     /locus_tag="PKJ16_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47498.1"
                     /translation="MDSFPIEKVFSILEWDTLRGHLEECCATPLGRGYAREMPFFTEI
                     PAISVQLGKITQIKALIEQDERPSFRGLSDIAPLAERAVRDSVLSLAELFQVREFAIA
                     TDRVRDFFAAHLESCPLLSDELAALHDTSGLRKELASSITDAQEINAARFPAIGRLTE
                     RIRATRSEIENRLHGIIHSPAGARYIQERTHAIRNDRYVLLVKSSMRTAITGTVHDIS
                     ASGATVYLEPQAVHELNNRLIAAELDLQVELHRILVQLTAAVKGAYDALTANLTVLAY
                     LEFLHAASCFSVAIGGSAPRIAETPLLRLYRARHPLLYLMETDKSRIISNDIFLGTDY
                     SCLIITGANTGGKTVLLKTAGLCALMARCGLHIPADPDSEIGVFGSILVDIGDDQSIA
                     ESLSTYSGQIVMIRSMLEAARADTLALIDEIIVGTNPRQGASLARAVLEGLIDTGAKI
                     IVTTHYNELKSLPAQDARFQNASVSFDLETLRPTYRLTIGIPGISYATEIARMYGIPE
                     RVLARARELIDDSDRSHDAVLERIQRYEEELTRDRETVGRLRDDLARQHRDIETARAE
                     LARLREEMKSEKGVAFLEELDGYRARVLERMRELQQAGAGETAGIKREIDEIHEAVTG
                     SVRDTV"
BASE COUNT         2161 a         3284 c         3010 g         2265 t
ORIGIN      
        1 gttcttgagc gtgagcgaac cgatcccctt tttgttgtac agctcgtatc gaacggcaaa
       61 aatgtcatca aacttcagat cctggacgat caccggcgac gcatcgcgcg gaatctggct
      121 tccgtatccg ccatggaggt tgtaacccgc ggcgaggttg tagctgatcg cggtatactg
      181 ctccccgaac atgtggtaat aactatattg cccgtatacg cgcagggaga aggaggacga
      241 tatcggtatg atcaccccaa ggctcgcgcg ggccagatag tcggcatgag ttttcgaatc
      301 ctcgatcgat gaaagcttca aggtgctata gtaccctccc gcgctcacgc caatcgaagg
      361 gtacagccag tcgaagagcg gataatacaa caccggaccg gcatcagccg tataaaacac
      421 cattttggtc tcggggtttt ccttgagctg catcgatgaa aacccgaatg tcccttcgaa
      481 gaaaaagaac tgccagccga atagtgtcgc gaagaaatat tcgggcggga gataatatag
      541 ggacaatccc gccgcaatcg cgttatcgaa gtattttcca aagaatcctc cggggacgac
      601 atagccggag ttcaccacat aggtgagatc gttgctgtaa acattgagat tggcggcatt
      661 cgctcgattg gagcgtacca tgacgccggc aaccaccatc accaatgccg ctgccgtaac
      721 aaccgcgcac cacgtccgct tgtttgcatg ccccagccgc atcataaaac ggccactaca
      781 attgctccaa aagctttatg gcctcggggt acgcctcgct gccacgaaac cgatccttga
      841 ttatcataag atcttttttc gcccgttccg catcgcgaag tttgatgcaa ttacgcgcgt
      901 gcaataaata ggcgttcggc agggtgtcgt cgtcggagga ttcccgcacg taacgcgcaa
      961 gatatttatt cgaggcggta tagtcgttta tgcaaaaaaa agcgaaaccg gcattgaaca
     1021 ttgcctcgga tcgtagacgg ggatcgcccg acccatctgc gatgcgcgcg aacgtgcgtg
     1081 acgcctcacg gtaatcgcac atccgcaaaa atgatatccc ggcgagatac tcgacgacat
     1141 cgcgtttctt tttcaccgcg ccggcctccg tgcgatacaa ctgcagggcg cgctgatacc
     1201 tgccgccttc atactccttc ttgatgcgat tgatgatcgc atcctcgtcc accggcggtg
     1261 catcagaaga tccgtcggcc atccatcgcg tatggtcatg aacgggatcg gccctgacag
     1321 cgatcacccg agtgaccgtg ttgatgcgag tgttcgcatt aaaaaccttt tttattattc
     1381 cggagaaccc gtgcttttgc gtttccgctc cctgccggtt cgaccgcttg ataacatccg
     1441 cgaagggaac ggtcgagttt tcatcgatgg tggtcgttgc gcttcccatg cgtatttcga
     1501 caaacgatct atcgagagtt tttacgatcg atccgggcga tagcacgagc cccactcggg
     1561 cgggaacggt ttttccggcc gtcaccaggg acaccgtgcc gataaagaat tcaatttttg
     1621 gcggggtggt gccgttgata tgctttcccg aaagaagaac agcgatcatt acgaaaatga
     1681 ttacacgcgt gcgcatctga ggactcccgc tatggacgct tttccacgat tttgtattat
     1741 tccaatttat acgtgtttcg ctttttgtca aatattaatc caatcatcct aatttaaatc
     1801 acggtggttc ttcgcatcaa agttttcatc gctgtaaata tctattgacg tcacccggcg
     1861 tgctattcca atggtgaaga cttcaatcct ccacacggag cgacacgcat gttccaatcc
     1921 gtcgaccagg ccatcgcctc tctcgaaaaa gagaagtaca tttgctcaaa agaaatcgca
     1981 accactgtgt acctcgcgat gaagatggaa aagcccatcc tcgtcgaggg gcccgccggt
     2041 gtggggaaaa ccgagcttgg caaggtgatt gcgagctcgc tgggcatgga cctgctgcgc
     2101 ctgcaatgtt acgaggggct ggacgaggcg aaggcgctgt acgaatggga gtatgccaag
     2161 cagctcctgt acacgcaaat cctcaaggac atgctgggat cgatgctcag caacgagaag
     2221 accctggccg acgcggtgaa aaagctttcg ggcgaggagg acgtgttctt ctccgataaa
     2281 ttcctgcttc cgcggcccat cctcatgtcc atcatgtcga agaagccgac gctcctgctc
     2341 atcgacgaga tcgacaagtc ggactccgag ttcgaggcct tcctgctcga gttcctgtcg
     2401 gacttccagg tgtcgatccc ggagatcggg acggtccggg cggcaattaa gcccttcgtg
     2461 ctcctcacgt ccaacaatgc gcgcgagatg tccgacgcgc tcaagcgccg ctgcctgcac
     2521 ctctacatcg attatccctc gcaggagctg gaggagaaga tcgtgaccct caaggttccc
     2581 gaatgctcat cgcacctcgt ccgcgagctc gtgaagagcg ttcacgccat ccgcgcgctc
     2641 cagctcaaaa aggagcccag catcagcgag acgctcgact gggcgaaggc gctcgccatc
     2701 ctgggccgcg acgttctcga cgccgagacg gcgatggata cgctcaatct catcctcaag
     2761 tacgaaaaag acatcgagct cgtgaagaag aacgccggca aggtcttcgt gcattgatcc
     2821 gccatgatca gcgcctacgt ttcgcacaag aagatcaata aaaagtttta cgattttatc
     2881 gacatcctca aggacgccgg cgtccccgtc tcgaccgctg aaacgctcga cctcttcacg
     2941 gcgctgggac atgtcggctt cgatgaccgg catgtcttcc ggcagaccct gcggacctcg
     3001 cttgtcaagg actacacgga catcccggtg ttcgaccggt gtttcgacgc tttcttcggg
     3061 aaaaaggggc ccgcgaaggg atccgacctg ccctctgcgg tcgccggcgc catggagatg
     3121 gagtcgcttc gcgatgaagg cctgagcgaa caggacctcg attccctgac ggcgctgctc
     3181 gacgatttca tcgaatcgct cgatgacgcc attttcttcg aacggaccta tcaggagatc
     3241 ctcacccttt tccttgaaga tcccgtggtc gcctcatcgt cgggggcgtt gggcatgatc
     3301 atcttcaaca cccgctcgcg cggccagcac ggaccgatgg gcgacagcgg gcagcggccc
     3361 gacgagacga tcaacctcaa ccaggtgatg ctccgtcaga tatcccgccg ggtggacgag
     3421 cggaagatcg gtacgcagat caaggaccgc gagcagtacc tgctcaacaa gttcatctac
     3481 cagttgaagc ccgacgaggt gaaggagatg aaggagctgg tgcgccgctt cgggcaaaag
     3541 ctcaagaacc ggatctcgct gaggaaaaaa cgcgtgaagc acggcgggat cgacgtgaaa
     3601 cgcacgatcc gcaagaacct gcagttcggc ggcacgccct tcaagattta tcacaaggac
     3661 cgcaagatcg accgcccgca gctcgtggtg ctctgcgata tctccggctc cgtgaaccag
     3721 tactcacgct tcatgctgct gctcacctac accctgcaga gccttttttc aaaggtgcgc
     3781 accttcgcct tcatcagcaa catggtggag atcacgccgc tgttcatgga gatgaacccc
     3841 gataccgcgc tcaactcgat attcggcgat accgatttca cctatggctg gggcagcaac
     3901 tacgggcgct gcttcaacat gttcgtccgc gagtacagcg attcgctcac cagcaaaacg
     3961 acggtcctgg tccttggcga cgcgcgcaac aacaaccagg accccgggct cgaatcattc
     4021 attaaaatca cggagcggac gcgcaatgtg ttctggctca atcccgaaaa gcgacacctg
     4081 tgggattgga acgacagcat cgccagcatc tatcgcgaac agtgcacgga aatgcgcgag
     4141 gtcaacagtt ttattgattt gtccgagttc atcgacaagc tgtttacgcg gtaaccatcg
     4201 ccaccgtttt tttagggcat tttacccaaa accacttgac ataatttttt ggctgggaaa
     4261 tacacattaa caaaaagcga tatcgcgtct gatgggcccc agacaggaga ggccgacata
     4321 aaagaccgtc tccggagtta accgatggaa attgaatttc gagatatggg cgaacacaag
     4381 attatccagg tgaatggcga ggtcgacctg tataatgtga gcgagctgaa gaaggcgctc
     4441 ttcagcgtca ccgacggcac gtcccggagc gttatcgtcg acatgaaaaa cgtcaattac
     4501 atggattcgt ccggcatcgg cgcattggtg gccggacaga aaaagatgaa ggcccataac
     4561 ggccgtttcg gcctgctcaa tatccacgag gacgtcctca acatcctcaa gctcgccacg
     4621 ctcgaccggt ttttcaaaat ctacgagggc gaggacgaac tcatttaacc cgccgcatcc
     4681 ctacacgacc ttgagcacca gcgtggtcat gtcgtcgtgg gggctcgcgt tcccgcgaaa
     4741 gtcgttcaca tcggccacaa tcgcctcgac gatgtcgttc gcgttgcgtt cgttgttgtc
     4801 gattatcagc ttctgcagcc gagcgcgccc atactcgtcc ctcttttcgt tgcgcatctc
     4861 cgtcacgccg tcggaataca tcaccacgat gtcgccgggg ttgagcttca cgcgcgcctg
     4921 ctggtagtcc acgtcctcgg tgatgccgat gggcatcccg tcgagcttgg tgatggtgca
     4981 ggcgcccatc gaggcccgat agcagaagag cggcccgtgc ccggcgttgg tgaaggtgag
     5041 ctcgcgcgtc cgccggtcat agatgagaaa aaacagcgtc gcgaatttgt cgatggcgaa
     5101 atcggcactc agggaatcgt tgatggcgcg caccacgctc gcggtgctga tgtccttccg
     5161 gtggatgtag gtcacgaaca ccgtgcggat catcaccatt acgagggagg ccgggatgcc
     5221 cttcccggac acgtccgaga ggagcgcccc gacgcgatgc tcatcgatgt ccacatagtc
     5281 gaagtaatcg ccccccaccc ccttggccgc tttcgtgtat ccttttatct gtacgccggt
     5341 cttgtcgtag aaggccatcg ggttcaggcc ctcctggatc tccttggcca ggtccaactg
     5401 ttcctgcatg atccgctgct cgatctcgat gtcgcgcgcc ttgcgcaagt gttccgtcat
     5461 cgcattgaat tcattggcca gctggccgat ctcgtcggac gacgacacgg tgatcttgta
     5521 gtccagcttt ccggtcccga tgatcgccgc gccctccgag agttgccgga tgggcttgat
     5581 gatgaacgac gcgagcgcaa ccgcccccac cacggatacg ccgatgaaca acaacgcgat
     5641 gaggatgatg ttctgggcca tgctcctgat ctctttgcgg atgatcccat cggaaaaacc
     5701 gagccggacc gagccgatac gttttttccc cagcttctcg acgagcggct tcgaaaaatc
     5761 ataaatgagc ccgccgccct cggggtcggg cagcgtgtgc cgcagcggtt cgcggagttc
     5821 cgtgtagttc ttgagggccc gggtgatatc gtcgtcgagc accgtgccgt ttttttccgg
     5881 gatgaggttc tgccgtatcc ggttgtctgc gtccaggatg taggcgtact tgatcgactc
     5941 gaggcccttc atgtcctcgg taatggccag gagctgcagc tcgctctccg tggagagcgc
     6001 gtcggccgcg gtcgtctgca tgtggtcgat ctcgcgccgg gcaagcgtga ggatctcctt
     6061 ttcgagcacg ccgctctcgt gccagatcac gtagatcgac attgtgatga tgatgaggac
     6121 cacgagggaa atgatggcga tggcgaactt gatcctgaga ctgaacttga cgccggacgt
     6181 gaccgcagcg tccgcatccg acgacgccgt cccggccttg tgctttaccg gcttggccgg
     6241 gcgcgccgcg tccggatcag caccgttctt tttcatgttt tccgccgcca ttattttttc
     6301 atgagtgcaa gcgcgcgctg gatgttgttg cgcgccttcg cgttcgcggg atcgatggcc
     6361 agcaccttcc gccattcctc gatcgcgcgg ttgagattat tgttcgagta aaagttgatc
     6421 ccccggaaat agtgatatcg gaccatctgc atctgcttgt ccgtcagccg cgactcgggg
     6481 cgctggtctt cggtcgttcc gatgcgcagc tgcgactcga tgcggttcac gctgatgagc
     6541 gcgcgcgaat tgttcgggtc gcgcgcgacg atccagcgaa actgctcgag cgccttcacg
     6601 aaattgtcgc gtccgccgcg ccggtacaga tcgagcccga gctggtagcg gctctcgatc
     6661 tcgccgggcg tcgcgcccgc gacctgcttc ttctccgcga gtccgtcctc cgcctgccgg
     6721 atcaaccggt cgatgtcggg atagtccggc gagagcgagc ggatgagctg gaacttgttc
     6781 agcgcaagct ggaagttctt ggcccgcaga agctgacgcc cctcgtcgac gatctcggac
     6841 gagaaccgtt cgtacgattt cgggttgagc cgcaggtcgc aacgcagctt gtactcgatc
     6901 gcgatccggt tcttggggaa gagggtcagg atcttctccc agctcttttt cgactccgcg
     6961 aaatcggcct tgtcgtagag cagcttgccc gaaacgacca gcgagttcac gatgctatag
     7021 tatggggaat tttcgtcaac gacttcctct tccagctgtt tgagggcctc ctgggccgtt
     7081 ttgatgtaat cgcgggcgag cttgtttccc gggtccatgc ggatcacttc ttcgaagtcg
     7141 gcaaccgcat cgcggtagcg cgacgccgag agcttcacca gcccgctctg gaacttctcg
     7201 tccacctggc gcagccggag gcgcttgagc tgctccgcgt attcggcctt gagccgcttg
     7261 atgctggcca gccgctcctt ggtgtccttg tagttgtcga tgagcgccag cacgttctcg
     7321 agatcgtcct cggcctggct gaagtttttc gcgttgaggt tggcgatgcc cgagacatag
     7381 aacttctcgg cctgaagacg gcgcagctcg aagttcttac ggttttccag gcggtcgtcg
     7441 agctcctcga tgagattgcg cgcctgccgg ttcttcgggt cgatgcgcag caccgcctcg
     7501 aactcgagct tcgcctcgga gtatttctcg tttttcatca tttccttgcc ggagttgatg
     7561 aggcccatga tctgttcgta gcggatgcgg ttgctgatgg ccagctccgc ccggcgcttt
     7621 ccctcgagcg cgacggggtc gtcggcgaag aactccagga tgtcgttcca gtgcttgagc
     7681 gccagctcgt actgccgtgc gtccatcgcc tgctcggcca tctccaggat ctgcgcatac
     7741 cggcgcttga gctcctcgga gagcttctgc tgggcctcgg ccaggcggat gtcgtcgtcc
     7801 agacgccgga tctcctcgcg aaggacctgc accggaggat cgcggggatc gatccggtag
     7861 gcgatcacga aattgttcac ggcgacgcgc gcgttcgatt tgccgaccct gaagtttttt
     7921 agcagcgttt tgcgcgccag ccggaggtgc tttcgcgaca tccagaaggc gaaatccttg
     7981 cgctgcttct cctcgtaggc atactccacc cgacgctgga cgcgatcgtt ctccgggtcg
     8041 agctcgagaa tccgcatcca ctcgttgatg gcgcgcgtaa actccttgtt gtcgtagtac
     8101 tcctgtgcgc gccgctccat ctcggaaatg gtctgcgcga gggcgggacc cgcggcggac
     8161 aagaagaccg tgaccgccag cgtccatgca accgttgtcc gaatgatgcc ccgcatgtcg
     8221 acccgcccgc tccggatggc cccctatcgg ttgatgtaca tcatcgtccg gttggagatg
     8281 agccagccgt cgcccgcgcg cttcccgtcg aggaacttga tgagcacgac atcgccgcgc
     8341 agcagctgat cgggtttcgc ggtggcgcgc ttgacggagg tggcctcgaa gttcacgtat
     8401 ttcctgatga accggttccc gtgctggtcc ttccgcacgt ccttctcgtc gacctcgctg
     8461 acctcgttca ccatcacatc catgaagtag ttgttccgga acatctccgc gaacaccgcc
     8521 tcatcgcggg gataggcctc gtatttccgg tagccatcgt acttcaggtc cttgatggcc
     8581 gcggcccaat tgttcaggat cgccgagggc tcctcggtcg aaggcgcctt tttacaggac
     8641 accgccgtcg cgagcagcgc gatggcggct atcagcatgc atgtccgttt cattaccgtt
     8701 cctctcacta aaaatgttat tgccctctgg gacaattatc ggatcacgtg gttacggttt
     8761 atcaaccgaa acgcaccgtc gagtaagcgc ataatcacgc gagtaaagac ccgtcaattc
     8821 aaatatacca atggattcct tcccgatcga aaaagtattc tcaattctcg aatgggatac
     8881 gctccgcggc cacctcgagg agtgctgcgc aacgcccttg ggacgcggat atgcgcgcga
     8941 gatgccgttt ttcaccgaaa tccccgccat ttccgtccag ctcgggaaga tcacgcagat
     9001 caaagcgctc atcgaacagg atgagcggcc ctccttccgg gggctgtccg acatcgctcc
     9061 cctcgctgag cgcgccgttc gcgacagcgt cctctcgctc gccgagcttt tccaggtccg
     9121 cgagttcgcc atcgccaccg accgggtgcg cgatttcttc gccgcgcacc tggaatcctg
     9181 ccccctgctg tccgatgagc tcgccgctct gcacgacacc tccgggctca gaaaggagct
     9241 tgcatcgtcc atcaccgacg cgcaggagat caacgccgcg cgcttccccg ccatcggacg
     9301 gctcaccgag cgcatccgcg cgacccgcag cgagatcgaa aaccgactgc acggcatcat
     9361 ccattccccc gccggggcgc ggtacatcca ggagcgcacc catgccatcc gcaacgaccg
     9421 ctatgtcctg ctcgtcaagt cgtcgatgcg cacggccatc acgggcaccg tccacgacat
     9481 atcagcaagc ggcgccacgg tctacctcga gccgcaggcg gttcacgaac tcaacaaccg
     9541 gctcatcgcc gccgagctcg atctgcaggt cgagcttcac cgcatcctgg tccagctcac
     9601 cgcggcggtc aagggcgcat acgacgctct cactgcgaac ctcacggtcc tcgcctacct
     9661 cgagtttctc cacgcggcgt cctgcttcag cgtcgccatc ggaggctccg ccccgcggat
     9721 cgccgagacg cccctccttc ggctttatcg ggcgcgtcac ccgctcctgt acctcatgga
     9781 gacggacaaa tcccgtatta tctcgaatga tattttcctg gggaccgact attcctgcct
     9841 catcatcacc ggcgcaaata ccggcgggaa aaccgtgctc ctgaagacgg cgggactgtg
     9901 cgcgctcatg gcccgatgcg ggctgcacat acccgccgat cccgattccg agatcggcgt
     9961 gttcgggtcg atcctggtcg acatcggcga cgaccagagc atcgcggagt ccctctcgac
    10021 ctattcgggg cagatcgtga tgatccgctc gatgctcgaa gcggcgcggg cggacacgct
    10081 ggcgctcatc gacgagatca tcgtggggac caatccccgg cagggggcga gtctcgcgcg
    10141 cgccgtcctc gagggcctga tcgacaccgg cgcgaagatc atcgtgacca cgcactacaa
    10201 cgagctcaaa agcctcccgg cgcaggacgc gcgattccag aacgcctcgg tctcgttcga
    10261 tcttgagacg ctgcgcccca cctaccggct caccatcggg atacccggca tcagctatgc
    10321 gacggagatc gcccgcatgt acggcatacc cgagcgcgtc cttgcgcgcg cccgcgagct
    10381 catcgacgac agcgatcgtt cccacgacgc agtgctcgag cgcatacagc gctacgagga
    10441 ggagctcacg cgagatcgcg agaccgtcgg acgcctcagg gacgatctcg cgcgacagca
    10501 ccgcgacatc gaaaccgccc gcgccgagct cgcgcgactc cgcgaggaga tgaaatccga
    10561 gaaaggggtc gccttcctcg aagagctcga cggctatcgc gcgcgggttc tcgagcgcat
    10621 gcgcgaactg caacaggccg gagcgggtga aacggcgggc atcaaacgcg agattgacga
    10681 gatccacgaa gccgtcacgg gctcggtgcg ggataccgta
//
LOCUS       DAORHF010000117        10683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5441_length_10683_cov_6.149228, whole genome shotgun sequence.
ACCESSION   DAORHF010000117 DAORHF010000000
VERSION     DAORHF010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10683
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5441_length_10683_cov_6.149228"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="PKJ16_10695"
     CDS             complement(<1..1415)
                     /locus_tag="PKJ16_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV47499.1"
                     /translation="MAKRVYFFGGAKADGDSTMKNLLGGKGANLAEMSKIGIPVPAGF
                     TITTEVCNEYYEIGRKFPAGLREEVMAAMKKVEQIMGARFGDSENPLLISVRSGARAS
                     MPGMMETVLNVGLCSKTIPGLIKKTNNPRFVYDAYRRLIMMYSDVVMEKAAGIEPAEG
                     MGIRQQLDRMLGDLKKSRGYKTDADISADDLKSLVERFKAKVREILGKDFPDDAYEQL
                     WGGIGAVFSSWNGKRAIAYRRIESIPDEWGTAVNVQSMVFGNMGDTSATGVAFTRNPA
                     TGDNKFYGEWLPNAQGEDVVAGIRTPNPINESTKNDQNKHLPSLERGMAPAYKQLDAI
                     RNKLEKHYTDMQDIEFTIQDGRLWMLQCRVGKRTGTAALNMAMDMLAEGLISKKEAVM
                     RVAPAQLDELLHPIVDPNAEKSATVIARGLPAGPGGACGEIVFTATDAVEAAKAGKKV
                     LLVREETNPEDVEGMRAAVGIL"
     gene            complement(1513..2475)
                     /locus_tag="PKJ16_10700"
     CDS             complement(1513..2475)
                     /locus_tag="PKJ16_10700"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV47500.1"
                     /translation="MNGFSRSDARGVVFKIIAANVVIFILQIFSESSGRPDPGGGMGP
                     GVSPMTHFLGLTPALVIGKGYLWQFFSYMFLHSTHSFFHIFFNMYAILIFGIPIEQAW
                     GSRRFLLYYVFCGTGAGVAIFLINLISGGMGFYLPTIGASGAVFGLLLAFGVLYPNME
                     LLLFFFIPIKAKYLVLLYGGLELYFELFGGASNISHIGHLGGLFFGIVFFLIFQRRTL
                     SFKGKIAQVRAQKKLNEYDSLMRAKPDYSDARGREEKLAMLTRVRAEGPEALSDDEYQ
                     RIKLLSIMTEDRGGPCPERLGESARCASCEDFDVCFLREIKRYL"
     gene            complement(2535..2906)
                     /locus_tag="PKJ16_10705"
     CDS             complement(2535..2906)
                     /locus_tag="PKJ16_10705"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV47501.1"
                     /translation="MTAPSKKPQSCVQCGTCCLADMIAYVTDDDLARWKREGRGDIMH
                     VIERESAVWMGDHLVSATDGHYIGPCPFLINRGDRYDCAIYETRPQVCRDYAAGSSEL
                     CSLFATTPGAPRSSRARNVID"
     gene            complement(3000..4613)
                     /locus_tag="PKJ16_10710"
     CDS             complement(3000..4613)
                     /locus_tag="PKJ16_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47502.1"
                     /translation="MEDIRILIVEDELIIAADIQRVLESLGFSVITAVSTGEEAIERA
                     GALRPDLVLMDIILADKMDGVEAAKQINEKFGIPVVFLTGNADGETVRRARESQPYGY
                     ILKPIHVQDLYSTIDTALSRFRLEAALHHQSEELEAANEEMEATNEELNAALEELEAT
                     NQELIRSQETLLESERRLQAIIDNAPFGALIYELFPENRLVFSGANPATDRILKMDHR
                     SLIGKTIEEAFPPLAQTEIPDAYRRAAATGERYSSEQVEYDDGGISGAYEVHAFQTER
                     NRMAVFFRDITERKRAEEQLCASEERYRELFEGIADSVFLHEALPDRPGTFLAVNDNA
                     CRTLGYSRDELLSMSVADIDVPEQAARIPAIMERLFRDGHALFETSHRTKDGRRIPVE
                     VNVRLLDLQGARVILSVARDIGERKRAQHYLDIAEVMMLALDETGAITLANRKCCAVL
                     GYAEHELLGKNWFDACLPPSERDAVKDVFRKIMSGELESVEYYENTVVAASGEQRRIA
                     WHNAIMRDEFGAPNGTLSSGEDITDRANP"
     gene            4826..6703
                     /locus_tag="PKJ16_10715"
     CDS             4826..6703
                     /locus_tag="PKJ16_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HNV47503.1"
                     /translation="MVRLKTIAALGAAVLVLAGCGAHEYAEYAAFPVGREARVAPGGE
                     GYLAVRIGIPADSYVYANPKGPGTGRAAHAEAKTAPGVLPGPARYLPGAPYTPQGEKE
                     HVLIYRRETTVFVPFTVRPDAAVGERTLSVHLETLVCTANTCAPKFSVIDFIVRIDAA
                     GPPRVHVDPALAALYRAASAPDGAIQRDPARDAERERIEIPRLVPRYTQSGSVAGIVQ
                     AIVFGLIAGFILNFMPCVLPVVSLKVLSLVRLAGERRARLVAHGLHFAMGILVVFTGL
                     ALAAAFLGAGWGALFQNQWFLVGMTAIVFALGLSLFHVFVITPPAFTERPGAASDASL
                     MSSFGKGMLATLLATPCSGPFLGGVLAWSLAQPPGVIFVVFVSVGAGMALPYVALTAF
                     PGLLRFVPKPGEWTIVFEQAMGFLLMATVVYLLGILEKDLLVPTLLFLLFLSAGLWQY
                     GRWGAVHQPIARRIISVTLLVVIATGGAVLSYRVLFDRDASPMTGAARTFTLRELYAS
                     RDAGRVSLVKFTADWCPNCKLVEKIALADASVARAIAAGGVDVYTADITVKNPEAEAL
                     LAALGSRSIPFLAVFPPGPAFAEPVCLRDMYSKGDVLEALELARPANAVPEMINIDIL
                     K"
     gene            6735..6944
                     /locus_tag="PKJ16_10720"
     CDS             6735..6944
                     /locus_tag="PKJ16_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47504.1"
                     /translation="MQLRLGEMLIADGSITEEQLNEALAAQNERDDLLGAILISLGYI
                     DEEILLDCLRRQGTIVKAKTRRDRA"
     gene            complement(6941..7531)
                     /locus_tag="PKJ16_10725"
     CDS             complement(6941..7531)
                     /locus_tag="PKJ16_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47505.1"
                     /translation="MEKRKTNIIDKKFQLRTTFSIIGVVYLVVSIIIAAIAINAALNN
                     RQLSELMQRHRGIISTQYETLSDVLALSRTRTWDKLESASEKMSRDIDRNMTQIDENI
                     ATMRRIYTSNYVLMIAVFVIVIVQGALLYILLIRKTHRISGPILIMTRQIEDIIEGRC
                     PDVRPIRENDEFRHFYERFGTMIEVLRDRYPRKKPD"
     gene            7619..8812
                     /locus_tag="PKJ16_10730"
     CDS             7619..8812
                     /locus_tag="PKJ16_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNV47506.1"
                     /translation="MGEDRARTRRIRQHVTAREHRFFAVVQPGFERIAQDELASLGAR
                     DAEAVEGGVEFSGRLEDCYRMNLCARTIVRVLMRLTGFRAERFERVREKVAAFPWELY
                     LAPGEPLAVRASARKSRLHHTGRLEEEVRGGIAERMGEGVMPDAARGDEGAQTVFLRF
                     ERDRCQVSLDASGALLHKRGYRHDAGEAPLRETTAAAIVLAARADAYDGIIDPMCGSG
                     TLAVEAALIATGLPPGAARGFAFEAWPAFRPAAYRHMKGKILEPLMPPALPRGRSILA
                     FDASDAAVVAARANRDAAGLTGCIDITRGDFFSLPRPFEGKTLVAINPPYGKRSMAGA
                     SVKSLYRRIGAVLRERYADAGYAIIVPGLECEKALGLPYDRKILFMNGGIRVAALIRD
                     AHYVT"
     gene            8979..9557
                     /gene="purH"
                     /locus_tag="PKJ16_10735"
                     /pseudo
     CDS             8979..9557
                     /gene="purH"
                     /locus_tag="PKJ16_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512841.1"
                     /note="involved in de novo purine biosynthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
     gene            9685..>10683
                     /locus_tag="PKJ16_10740"
     CDS             9685..>10683
                     /locus_tag="PKJ16_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006786979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV47507.1"
                     /translation="MAEVTLKEIYPVPEKFRQKAYIKSRADYEKMWKDSVDNPEAFWA
                     KIADEYITWFKKWDKVMDCNYGKTVKDLQVKWFTGSKVNVSYNCLDRHLEKRGDKIAL
                     IWEGNEPTEDKTFTYKQLHEQVCKFANVLKKNGVKKGDRVTIFLPMIPELAIGILACT
                     RIGAIHSVVFGGFSAEALRDRVIDCDSRVVVSCDGTFRGAKAVPQKDNSDAAIAQCPN
                     VKLQIVVKRVGDKIQCKWNDKIDKWWDAEMAAADAKCEPEQMDAEDPLFILYTSGSTG
                     KPKGVMHTTAGYLTYVAFTHKMIFDYHDGDIYWCTADIGWVTGHSYIVYGPLANGAT"
BASE COUNT         1874 a         3228 c         3483 g         2098 t
ORIGIN      
        1 aggatgccca cggccgcgcg catcccctcg acgtcctcgg ggttggtctc ttcgcgcacc
       61 aggagcacct tcttgccggc tttcgcggcc tcgacggcgt cggtcgcggt gaagacgatc
      121 tcgccgcagg cgccgccggg accggcgggc aggccgcgcg cgatcaccgt cgcgctcttc
      181 tcggcgttcg gatcgacgat ggggtgcaag agctcgtcga gctgcgcggg tgccacgcgc
      241 atcacggcct ccttcttgct gatgagcccc tcggccagca tgtccatggc catgttgagc
      301 gcggcggtgc cggtgcgctt gcccacgcgg cactgcagca tccacaggcg cccgtcctgg
      361 atggtgaatt cgatgtcctg catgtccgtg taatgcttct cgagcttgtt tcgaatggcg
      421 tccagctgct tgtaggcggg cgccatcccc cgctcgaggg acggaaggtg cttgttctgg
      481 tcgttcttgg tcgattcgtt gatggggttg ggcgtgcgga tgcccgcgac cacgtcctcg
      541 ccctgcgcat tggggagcca ctcgccgtag aacttgttgt cgccggtggc cgggttgcgc
      601 gtgaaggcca cgccggtggc cgaggtgtct cccatgttgc cgaataccat cgattgcacg
      661 ttcaccgcgg tgccccactc gtccgggatg ctctcgatgc ggcggtaggc gatggcgcgc
      721 ttgccgttcc acgacgagaa cacggcgccg atgccgcccc agagctgctc gtaggcgtcg
      781 tccgggaagt cctttccgag gatctcgcgc accttcgcct tgaagcgttc gacgaggctc
      841 ttgagatcgt ccgcggagat gtcggcgtcg gtcttgtagc cgcgggactt tttcaggtcg
      901 cccagcatgc ggtcgagctg ctggcggatg cccatgccct cggcgggttc gatgccggcg
      961 gccttttcca tcaccacgtc cgaatacatc atgatgaggc ggcgataggc gtcgtatacg
     1021 aaacgcggat tgttggtttt cttgatgagg ccggggatgg tcttggagca caggcccacg
     1081 ttgaggaccg tttccatcat gccgggcatc gaggcgcgcg cgccggagcg gacggatatg
     1141 aggagcgggt tttcactgtc gccgaagcgg gcgcccatga tctgctcgac ctttttcatg
     1201 gccgccatga cctcttcgcg cagacccgcg ggaaatttcc ggccgatctc atagtattcg
     1261 ttgcagacct ccgtggtgat ggtgaagccg gccgggacgg ggatgccgat cttgctcatc
     1321 tcggcgaggt tcgcgccctt gccgcccagg aggttcttca tcgtcgagtc cccgtcggcc
     1381 ttcgcgccgc caaaaaaata cactcgtttt gccatggtag tgactccttg tgtcagtggg
     1441 ttgtatgatt ccggaacacg taacagtaaa acgggtgccg gagaatcatg aaattgtaaa
     1501 cattaatttt ttttacaaat accgctttat ctcgcgcaaa aagcacacgt cgaaatcctc
     1561 gcaggaggcg caccgcgcac tctcgccgag ccgctccggg cagggtccgc cgcggtcctc
     1621 ggtcatgatc gacaggagtt tgatgcgctg atattcgtcg tcgctgagcg cctcgggccc
     1681 ctccgcgcga acgcgggtga gcatggccag cttctcctcc ctgccgcgcg catcgctata
     1741 gtccggtttg gcccgcatga gcgaatcgta ttcgttgagc tttttctggg cgcggacctg
     1801 cgcgatcttt cccttgaacg acagggtgcg ccgttggaaa atgagaaaga acacgatgcc
     1861 gaagaagagc ccgcccaggt ggccgatgtg cgatatattg ctcgccccgc cgaagagttc
     1921 gaaatacagt tcgagccccc catagagcag caccaggtat ttcgccttga tggggatgaa
     1981 gaagaacagc agcagctcca tgttcggata gagcaccccg aaggccagca ggaggccgaa
     2041 caccgcgccc gaggcgccga tcgtcgggag gtagaacccc atgccgcccg agatgaggtt
     2101 gatgaggaag atggcgacgc cggcgcccgt gccgcagaag acgtagtaga gcagaaagcg
     2161 ccggctcccc cacgcctgct cgatgggtat gccgaagatg aggatcgcgt acatgttgaa
     2221 gaaaatatgg aagaagctgt gcgtgctgtg gaggaacatg tacgagaaga actgccagag
     2281 gtagcccttg ccgatcacca gcgcgggggt gaggcccaga aagtgggtca tcggagagac
     2341 gcccggaccc atccccccgc cggggtcggg ccgccccgag gactcgctga atatctgcag
     2401 gatgaagatg acgacgttgg ctgcgataat cttgaacacc acgccgcgcg catccgatcg
     2461 ggaaaagccg ttcatgggcg gacacctgcc ccctgtcctt cgattttatg atacctacag
     2521 caaaggcgcg gtcatcagtc aatcacattc cgcgcgcggg acgatcgggg cgcgcccggc
     2581 gtcgtcgcga acagcgagca cagctcggat gatccggcgg cgtagtcgcg gcacacctgg
     2641 gggcgcgtct cgtagatggc gcagtcgtac cggtcgccgc ggttgatgag gaaggggcaa
     2701 ggcccgatgt agtggccgtc ggtcgcggag accaggtggt cgcccatcca caccgcgctc
     2761 tcgcgctcga tgacgtgcat aatatccccc cgcccctcgc gcttccagcg cgcgagatcg
     2821 tcatcggtca cgtaagcgat catgtcggcc aggcagcacg ttccgcactg gacgcacgat
     2881 tgcggttttt tcgacggagc ggtcacgacg gcccccttgt gcggtcactc tgcctccggg
     2941 ggcgcactat ggcaaggaaa gttattattt tttttgatga tatacgtcgc ccgcgcgggc
     3001 tacggattcg cccggtccgt gatgtcttcg ccggagctca gggtcccgtt gggcgcaccg
     3061 aattcgtcgc gcatgatggc gttgtgccag gcgatgcggc gctgttcgcc cgatgcggcc
     3121 acgacggtgt tttcgtaata ctcgaccgac tcgagttcgc ccgacatgat tttacggaag
     3181 acgtccttca cggcgtcccg ctcggagggc ggcaggcagg cgtcgaacca gttttttccc
     3241 agcagttcgt gttcggcgta tccgagcacg gcgcagcact tgcggttcgc gagggtgatc
     3301 gcgccggtct cgtccagcgc gagcatcatc acctccgcga tgtccaaata gtgctgcgcg
     3361 cgcttgcgct cgccgatgtc gcgcgcaacc gaaaggatca cccgcgcgcc ctgaaggtcc
     3421 agcaagcgca cgttcacctc cacggggatg cggcggccgt ctttcgtgcg atggctggtc
     3481 tcgaacagcg catgcccgtc ccggaacagc cgctccatga tggcgggaat gcgcgccgcc
     3541 tgctcgggga cgtcgatgtc cgccacgctc atcgagagca gttcgtcgcg gctgtagccg
     3601 agcgtccggc aggcattgtc gttcacggcc aggaacgtgc ccggacggtc cgggagcgcc
     3661 tcatggagaa acacggagtc cgcgatcccc tcgaagagct cgcggtagcg ctcctcgctt
     3721 gcgcacagct gctcctcggc gcgcttgcgc tcggtgatgt cgcgaaagaa caccgccatg
     3781 cggttgcgct ccgtctggaa ggcgtggacc tcgtaggcgc cgctgatgcc gccgtcgtcg
     3841 tactccacct gctcgctcga gtatcgttct ccggtcgcgg cggcgcgacg gtaggcgtcc
     3901 gggatttccg tttgtgcgag cggcgggaag gcctcctcga tggttttgcc gatgagggag
     3961 cggtgatcca tctttaggat gcggtccgtt gcggggtttg cgcccgagaa cacgagccga
     4021 ttctcgggaa acaattcgta gatcaacgcc ccgaaggggg cattgtcgat gatagcctgg
     4081 agccgtcgct cgctttcgag aagggtttcc tgcgagcgga tgagctcctg gttcgtcgcc
     4141 tccagctcct cgagggcggc gttgagctcc tcgttggtcg cttccatctc ttcgttcgcc
     4201 gcctcgagct cctccgactg gtggtgcagc gccgcctcaa ggcggaaccg gctgagcgcg
     4261 gtgtcgatgg tggaatacag gtcctggaca tggatgggct tgaggatata gccgtagggc
     4321 tgggactcgc gcgcgcggcg gacggtctcg ccgtcggcgt tgccggtcag aaagaccacc
     4381 gggatgccga atttctcgtt gatctgtttc gcggcctcca cgccgtccat tttgtcggcg
     4441 aggatgatgt ccatgagcac gaggtccggc cgcagcgcac cggcccgctc gatggcctcc
     4501 tcgccggttg acacggcggt gatcacggaa aatccgaggg attcgaggac ccgctggatg
     4561 tcggcggcga tgatgagctc gtcctcgaca atgagaattc gtatgtcttc catgattctg
     4621 tgtttgtgta ttccgcgcgc tatctgtatt gaacgtctgt tggtaaaaag agatatgttt
     4681 gtctcaaaat gtaaagcgaa ttttaccgcc atgttcgttc gcgataaaag agttgcatgc
     4741 gtatgcgcct gcgctatgat gcagaaattc cgcggtcgga agggcgtgtg ggcgttcaga
     4801 tcgcgcgatc gataaggagt ggagcgtggt tcgattgaaa acaattgcgg ccctgggtgc
     4861 ggccgttctc gtgctcgcgg ggtgcggagc gcatgaatac gccgagtacg cggcgtttcc
     4921 cgtcgggcgc gaggcgcggg tggcccccgg cggcgaaggc tatctggcgg tccgcatcgg
     4981 gatccccgcc gattcgtatg tatacgccaa tcccaagggc cccggcaccg gccgcgcggc
     5041 ccacgccgag gcgaagacgg cgccgggcgt tcttcccgga ccggcgcgct accttccggg
     5101 cgctccgtac acgccgcagg gcgagaagga gcacgtgctc atctatcgcc gcgaaacgac
     5161 ggtgttcgtt ccgtttacgg tacggccgga cgccgccgtc ggcgagcgaa cgctttctgt
     5221 gcacctcgag acgctggtat gcaccgccaa tacctgtgcg cccaaattct ccgtcatcga
     5281 tttcatcgta cggatcgacg ctgcggggcc gccgcgcgtt catgtcgatc cggcgctcgc
     5341 cgcgctctac cgcgccgcga gcgcgcccga cggtgcgatt caacgggacc ccgcgcgcga
     5401 tgcggagcgc gagcgcatcg agatcccgcg cctggttccg cgctacacgc aatccggatc
     5461 ggtggcgggg atcgtccagg cgatcgtctt cggcctcatt gcgggtttca ttctcaactt
     5521 catgccgtgc gtgctgccgg tggtgagcct caaggtgctc agtctggtgc gcctggcggg
     5581 ggagcgccgc gcgcggctcg tggcgcacgg cctgcatttc gcgatgggca tcctcgtggt
     5641 gttcacggga cttgcgctcg ccgcggcctt cctgggcgcc ggctggggcg cgctcttcca
     5701 gaatcagtgg ttcctcgttg gcatgaccgc gatcgtcttc gcgctagggc tatctctctt
     5761 tcacgtgttt gtcatcaccc cgccggcctt cacggaacgg cccggcgcgg catccgacgc
     5821 gagcctcatg tcctcgttcg gcaagggcat gcttgcgacg ctcctggcca cgccgtgcag
     5881 cgggccgttt ctcggcgggg tcctcgcctg gtcgctcgcg cagccgcctg gggtcatctt
     5941 tgtcgtgttc gtgtcggtgg gcgcggggat ggcgctgccc tacgtggcgc tcacggcctt
     6001 tcccggcctg ctgcgattcg ttcccaagcc gggtgagtgg accatcgtct tcgaacaggc
     6061 gatgggcttt ctgctgatgg cgacggtcgt ctatctgctc ggcattctgg aaaaggacct
     6121 gctggtcccc acgctgctgt ttttgctgtt cctgtcggcc gggctctggc agtacgggcg
     6181 ctggggggcg gtccaccagc cgatcgcgcg gcggatcata tccgtaacgc tgcttgtcgt
     6241 gattgcgacc ggcggcgccg tgctgtcgta tcgcgttctg tttgaccgtg atgcgtcgcc
     6301 aatgacggga gcggcgcgga ccttcacgct gcgcgagctc tatgcgagcc gtgacgcggg
     6361 ccgcgtgtcg ctcgtgaaat tcaccgccga ctggtgtccc aactgcaagc tcgtggagaa
     6421 gatcgcgctc gccgatgcgt cggtcgcgcg cgcgatcgcc gcgggcggcg ttgacgtgta
     6481 caccgccgac atcacggtga aaaatccgga ggccgaggcg ctcctcgccg cgctgggaag
     6541 ccggtccatc ccgtttcttg cggtgttccc gccggggccg gcgtttgcgg agccggtgtg
     6601 cctccgggac atgtactcga agggcgacgt gctcgaggcg cttgagctgg cgcggcccgc
     6661 caacgcggtc ccggagatga tcaatatcga cattttgaaa tagggtatat tattcgaaac
     6721 agagggggcg tgcgatgcaa ctgcggctag gtgaaatgct gatcgccgac ggctcgataa
     6781 ccgaggagca gctcaacgag gcgctggcgg cgcagaacga gcgggacgat cttctcggcg
     6841 cgatcctgat atcgctgggc tacatcgacg aggagatcct cctggactgc ctgcggcggc
     6901 aggggaccat cgtaaaggcg aaaacccgcc gggaccgcgc ctagtccggc ttcttccgcg
     6961 ggtaccggtc ccgcaggacc tcgatcatcg tcccgaagcg ctcgtagaaa tggcgaaact
     7021 cgtcgttctc gcggatgggg cgcacgtccg ggcagcggcc ctcgataatg tcctcgatct
     7081 ggcgggtcat gatgaggatg ggtcccgaga tgcggtgcgt cttgcggatc agcaggatgt
     7141 agagcagggc gccctggacg atgacgatga cgaacacggc gatcatcagg acgtagttgc
     7201 tggtgtagat gcggcgcatg gtggcgatgt tctcgtcgat ctgcgtcatg ttacgatcga
     7261 tgtcgcgcga catcttttcg ctcgcggatt cgagcttgtc ccacgtgcgg gtgcgcgaga
     7321 gcgcgagcac gtcgctgagc gtttcgtact gggtggagat gatgccgcgg tggcgctgca
     7381 tgagctcgga gagctgccgg ttgttgagcg cggcgttgat ggcgatggcg gcgatgatga
     7441 tggacaccac caagtacacc acgccgatga tggagaaggt cgtgcgcagc tgaaattttt
     7501 tatcgatgat gttcgttttt cttttttcca tttttcctcc cgcgggcgcc ggatagggta
     7561 gcatggcggg gcggtgtgct gtcaagttga aattcccgaa cacgggcaag gtgcaggtat
     7621 gggtgaggat cgggcgcgta cgcgccgcat tcggcagcac gtgacggcgc gcgagcaccg
     7681 cttcttcgcc gtggtgcagc ccggcttcga gcgcattgcg caggacgagc tcgcgtcgct
     7741 gggcgcgcgc gacgcggaag cggtcgaggg cggcgttgaa ttttccggcc gattggagga
     7801 ttgctaccgc atgaacctct gcgcgcggac gatcgtccgg gtgctcatgc gcctcaccgg
     7861 cttccgcgcc gagcgcttcg agcgggtgcg cgagaaggtc gccgctttcc catgggagct
     7921 ctacctcgcg ccgggcgagc cgctcgccgt gcgcgcgtcg gcgcggaagt cgcgcttgca
     7981 ccataccggg cggctcgagg aggaggtgcg cggcggcatc gccgaacgga tgggggaggg
     8041 ggtgatgccg gacgccgctc ggggcgacga gggcgcgcag acggtgttcc tccgttttga
     8101 gcgcgaccgc tgtcaggtga gcctcgacgc gagcggcgcg ctccttcaca agcgcggtta
     8161 ccggcatgat gcgggcgagg ccccgctccg cgagacgacg gccgccgcca ttgtactcgc
     8221 cgcgcgcgcg gacgcgtacg atggcatcat cgatcccatg tgcggctccg ggaccctggc
     8281 cgtggaggcg gcgctcatcg cgaccgggct tccaccgggg gccgcgcgcg gtttcgcgtt
     8341 cgaggcgtgg ccggcattcc ggccggccgc gtatcgacac atgaaaggaa aaatactgga
     8401 accgttgatg ccgccggcgc tcccgcgcgg cagaagcatc ctcgccttcg atgcaagcga
     8461 cgccgccgtc gtcgccgcgc gcgcgaaccg agacgcggcg ggcctcacgg gctgcatcga
     8521 catcacgcgc ggggactttt tctctctacc gcgcccgttc gaggggaaaa cgctcgttgc
     8581 gatcaatccg ccgtacggga agcgctccat ggcgggggcg tccgtgaaat cgctctatcg
     8641 gcgaatcggc gccgttttgc gcgagcgcta cgccgatgcg ggctacgcga tcattgtccc
     8701 ggggcttgaa tgcgagaagg ccctgggcct tccctacgac cggaaaattc tttttatgaa
     8761 cggcgggatc cgcgtggccg cgctgatccg ggacgcgcat tatgtgacat aattattccc
     8821 ggggtggatt gcaagccgat tggtcggcgc aatagtgtac aaaaaaatca atatttccaa
     8881 gaacgccaaa aaaagcttgt ttttttaagg tctggaattc aaatggtctc cgtttcaaat
     8941 cgccgatcaa agacacgaag cgcagcgtat cggactgaat ggcacagata acacgggcac
     9001 tcatcagcct ctctgacaaa agcggtattg tcgattttgc gaaggagctg aatgcgttcg
     9061 gcgtccagct cctgtcgacg ggcggcacgg ccgcccagtt gcgcgcggcg gggcttccgg
     9121 tcatggacgt gtcggagtac acgggttttc ccgagatgct cgacggcagg gttaaaacgc
     9181 tccatccgaa ggtgcacggg ggcctgctgg gcgtccggtc aaacgcagaa cacgtcgcca
     9241 ccatgaagaa gcacggcata gagcccatcg acatgatcgt cgtgaacctc tatccgttca
     9301 aacaaaccat cgccaaaccc ggctgcacgc tggaggacgc ggtggagaat atcgatatcg
     9361 gcggcccggc catgctgcgc tccgccgcga aaaatttcga gagcgtcacc gtcgtcaccg
     9421 acccgaccga ttattcggcg attgtcgccg agatgaagcg caccggcggt tcggtatccc
     9481 gcgaaacgaa catgcggctg gcggtcaagg tgtaccagac cacatcagcc tacgacggaa
     9541 tgatcgccga gtacctgcac acaaatctta attgaatcaa attcaattaa gaaaaacttt
     9601 ggattgcctg ttattcttca acatttggta tcaacggtaa ccaggcgtcc gttacaatac
     9661 taaatcaaag tacggaggta tttaatggct gaagtaacgc tgaaagagat ttatccggta
     9721 ccggaaaaat tccggcagaa ggcgtacatt aagagccgcg ccgactacga gaagatgtgg
     9781 aaggattccg ttgacaatcc tgaggccttc tgggcgaaga tcgccgacga gtacatcacc
     9841 tggttcaaga agtgggacaa ggtcatggac tgcaactacg gcaagaccgt gaaggacctg
     9901 caggtgaagt ggttcaccgg ttcgaaggtc aacgtgtcgt acaactgcct cgaccgtcat
     9961 ctcgagaagc gcggcgacaa gatcgcgctg atatgggaag gcaacgagcc cacggaagac
    10021 aagaccttca cctacaagca gcttcacgag caagtgtgca agttcgccaa cgtgctcaag
    10081 aagaacggcg tcaagaaggg cgaccgcgtc accatcttcc tgcccatgat ccccgagctc
    10141 gcgatcggta tcctggcctg cacgcgcatc ggcgcgatcc actcggtcgt tttcggcggc
    10201 ttctccgctg aggcgctccg cgaccgcgtt atcgactgcg acagccgcgt ggtcgtctcc
    10261 tgcgacggca ccttccgcgg cgcgaaagcg gttccgcaaa aggacaattc cgatgcggcg
    10321 atcgcacagt gccccaacgt gaaactgcaa atcgtcgtca agcgtgttgg cgacaaaatt
    10381 cagtgcaagt ggaacgacaa gatcgacaag tggtgggacg ccgaaatggc ggccgctgac
    10441 gcaaagtgcg agcccgagca gatggatgcg gaagatccgc tcttcatcct gtacacttcc
    10501 ggctcaaccg gaaagcccaa gggcgtcatg cacaccaccg ccggctatct gacctatgtt
    10561 gccttcaccc acaagatgat cttcgactat catgacggcg acatctactg gtgcacggcc
    10621 gacatcggct gggtcaccgg ccactcctac atcgtctacg ggccgctcgc caacggcgcg
    10681 acc
//
LOCUS       DAORHF010000118        10682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5443_length_10682_cov_5.941846, whole genome shotgun sequence.
ACCESSION   DAORHF010000118 DAORHF010000000
VERSION     DAORHF010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10682
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5443_length_10682_cov_5.941846"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="PKJ16_10745"
     CDS             complement(<1..1393)
                     /locus_tag="PKJ16_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV47508.1"
                     /translation="MDLTTLYNLPAETITRLSFFNVGGPHGFMRWAIYLLMFIAFGYI
                     IYTIYQRVKVWRVGKPELRTDFPEKRIMAVIKYVFLQGKIVREAYAGIMHAALFFGFV
                     GLFIVTLLIMIQDDFTELFFHIKFLKGTPYLLWSLAGDLFGVVVIVGLVMAFYRRYKV
                     KPSRLDTKPIDTFALVLIALIIFTGFMTEALRIAISAFPAFEVWSPVGYAMAYLFAWM
                     SVPALETVHYLTWWVHVLGAFVFIGLVGSDKLGHILISSLNVYFQNLDNESAKTKFAM
                     PVINPTEFETAESFGVGAVENFTWKQLMDGDACTRCGRCQDNCPAYLTEKPLSPKKIQ
                     NDVKAAMDYRVPMLMAAEDPAAVEDEKMLIGDFVTEDEIWACTNCAACMENCPVQLEH
                     VPKINDMRRYKILMEGAMAGELQSSLTNMESNSNPYGFAFAARGDWVAAQGLDIKTIA
                     EDPNVDFVYYVGCA"
     gene            1702..2880
                     /locus_tag="PKJ16_10750"
     CDS             1702..2880
                     /locus_tag="PKJ16_10750"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017866498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HNV47509.1"
                     /translation="MTTPEAHDSTSVGIVRTKHLHFAEPPNELVLTSGNKLGPITLAY
                     ETYGALNADKSNAILVLHALSGTAHAAGYFTHEDRYPGWWDTYIGPGKAFDTDKYFII
                     SSNVIGGCNGSTGPASINPASGREYGIDFPLVTIQDMVNAQWHLVTHLGIDRLLAVAG
                     GSMGGMQALQWTISYPDMVQAAIAIGTAASISAQGIAFNEVGRQAIYRDPHWNNGAYY
                     GGPPPKNGLALARMIAHITYLSEDIMHEKFGRRLQNTEMLSFAFKEEFMVESYLHQQG
                     IKFVNRFDANSYLYITKAIDYFDLKSSHGGSLIAAFENVRSDFLVISFSSDWLYPTPL
                     AKEIVKALRANGAHVIFTEIETSKGHDAFLLENEMLERNIANFLRSEYSKRTNTNPDR
                     "
     gene            3045..3680
                     /gene="metW"
                     /locus_tag="PKJ16_10755"
     CDS             3045..3680
                     /gene="metW"
                     /locus_tag="PKJ16_10755"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HNV47510.1"
                     /translation="MSTTRIGYDLIIEEIPERSRVLDLGCGDGELLTELADAKRVNAY
                     GVEISAEGVSRCLEKGLYVYQGDIDEGLADYRSGSFDYVILNQTLQDTKRPLYVITES
                     LRIGRFAIVSFPNLGYAANRFQLMFRGVMPKNHLFSYNWYDTPNIHIMSIRNFSELCR
                     ENRYPIQTERHFSMRPHGSVHRRRVFPNLFAEYGFFILRGRDAATRPQETS"
     gene            3677..4135
                     /locus_tag="PKJ16_10760"
     CDS             3677..4135
                     /locus_tag="PKJ16_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV47511.1"
                     /translation="MKQVAEYTKREFIRVLSADEKFAAIEELAAVFAGSSVCGDLGAL
                     VESLREREKIMSTGIGFGIAIPHAKIASVKEIAFAVGISPRGISYDSMDGEPVHLIIL
                     VAAGERQHKDYLRMLSNIMTILKNPAVKERIISSQSPDEVLAILKSAPGK"
     gene            4224..4670
                     /locus_tag="PKJ16_10765"
     CDS             4224..4670
                     /locus_tag="PKJ16_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47512.1"
                     /translation="MKRIAIAVLLFFAVGLAAQQNYQKSTAMSFKLKSISASKNGPSK
                     IGMFRSKQVVWLNIEIIGLRKDADNNFSFQSDLTLNDPDKQTILNVPNILNQQLNAPD
                     ANTVVANYNINLGGQMKPGKYDVTIVFRDVNAVTYSDFKASFFVSE"
     gene            4808..7276
                     /gene="leuS"
                     /locus_tag="PKJ16_10770"
     CDS             4808..7276
                     /gene="leuS"
                     /locus_tag="PKJ16_10770"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HNV47513.1"
                     /translation="MAEREYSFKDIELKWQSTWREKNIFAATRDASKKKYYLLEMYPY
                     PSGRIHMGHVRNYTIGDLMARYRRMNGFNVFHPMGWDSFGLPAENAAIQNNIPPKTWT
                     DKNIASMKAQFDRMGFSYAWEREIMSHDPAYYRWNQWMFCRMFEKGLAYKKVAPVNWC
                     PKCNTVLANEQVEDGLCWRCESTVTQKELNQWFYKITDYAEELLSAHDDIRAGWPERV
                     LTMQKNWIGRSTGLMINFTVDDGTPFPIFTTRPDTVYGVTFMVIAPEHPFLKRVTDPA
                     VLAFIERLKAQSLVDRVSEEKEKEGIDTGIKIVNPFNGDRVPLFVGNFVLMEYGTGAI
                     MAVPAHDTRDFAFAKKYGIAIKLVIDNPASPIDVSAMMDAYVDEGVCVNSGPFTGVPN
                     RAAMEKIAAYAETEGFGAREVQYRIRDWLISRQRYWGCPIPAIYCDACGTVMPRDEDL
                     PITLPTQVDFKGDSVSPLVSMPSFHNTTCPKCGGPARRDTDTMDTFVDSSWYFAKFTS
                     AGSPGMFDTDEVDYWMPVDQYIGGIEHAVLHLLYARFYTKVMADFGLIKFREPFTRLL
                     TQGMVIKDGAKMSKSKGNVVDPDKIIEEYGADTVRLFMLFASPPDKDLDWSDKGVEGC
                     YRFLTRVWRMVGRHGERYRPAPSFDGIELSKALKTLRGDLHRTIKAVTNDIEERLQYN
                     TAIARMMELVNAVYQTDEVELASDHGALVVSEVFDALIPLLAPFVPHIADELWQILGH
                     DEILFSHPWPSFREELTARDEVEIVFQVNGKIRSRDLVPADIPRDQMEKLALENPRVK
                     EFIAGKQVVKVVIVPGKLVNVVIK"
     gene            complement(7400..7813)
                     /locus_tag="PKJ16_10775"
     CDS             complement(7400..7813)
                     /locus_tag="PKJ16_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47514.1"
                     /translation="MAMNPEQLLQVYVLSLGIFLISVAGMELIAPFTMFQVWRRWVSH
                     RIFPLYGVSLVIMGFPLTQYRHADIPAIGYVVFAIGLVVVFSGPFILLYPEKIRAMVS
                     EIAETAGEDGVRRLVFIDAAMRLIAGGVFVYSYFA"
     gene            complement(7843..8247)
                     /locus_tag="PKJ16_10780"
     CDS             complement(7843..8247)
                     /locus_tag="PKJ16_10780"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HNV47515.1"
                     /translation="MERRDAKPYVLCAAAALGLAVFGNDTLCFFKNIYGAPCPGCGLT
                     RAYLSLVRGEFFLALRYHPLFALAPVVLVVWAFRDRRPFDRFYRSDRFWIAIIVALLA
                     TWAVRMVLYFPDVPPMDLNRSALFMRIFDAVR"
     gene            complement(8260..8628)
                     /locus_tag="PKJ16_10785"
     CDS             complement(8260..8628)
                     /locus_tag="PKJ16_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HNV47516.1"
                     /translation="MTEKPEVRSPAAVIVFTLITCGIYGLVWIFKFSKEIKNYLNKEE
                     INPGLELLLCLVCFPYAIYWSYKFGKLMMEAQQKAGQPVEDNAILYLVLACFGLIMVN
                     MAIMQEAANKIWTAQGLQAQ"
     gene            complement(8797..9417)
                     /locus_tag="PKJ16_10790"
     CDS             complement(8797..9417)
                     /locus_tag="PKJ16_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNV47517.1"
                     /translation="MIHKNVVTDFSPEAHAPRIDPTAYVHPLGAVIGNVSIGRRVFVS
                     PFASVRGDEGQPLHVGDESNVQDGVIIHALETFHEGHAVEKNLVEVQGKKYAVHVGDR
                     VSLAHQVQVHGPAAIGNDCFIGMKVLVFKSRIEDGCVIEPSCVLMGVTIKAGHYVPAG
                     SVLANQADADRLSKKITDDYAFRTLNAGVVHVNTNLADAYRKAGIQ"
     gene            complement(9607..10215)
                     /gene="lexA"
                     /locus_tag="PKJ16_10795"
     CDS             complement(9607..10215)
                     /gene="lexA"
                     /locus_tag="PKJ16_10795"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HNV47518.1"
                     /translation="MKNKLTDKQELIFSFIKESIRTSGFPPTVREIGERFQITVKGAY
                     DHLKAIEKKGFIRCNQNKSRAIELLVDRADTEAPDVVSIPHVGRIAAGAPILAQENVD
                     EYLCFPRAMFRSGEFFALTVKGDSMIDAGINDGDIAIIKKQKTAVNGDIVAALIEDEA
                     TLKVFKKEKDRIALNPANAAYRPIVLESVDILGKLAGLFRSY"
BASE COUNT         2327 a         3397 c         3015 g         1943 t
ORIGIN      
        1 ccgcgcaccc gacatagtac acgaaatcca cgttcgggtc ttccgcgatc gtcttgatgt
       61 ccagcccctg cgccgcgacc cagtcgccgc gcgccgcgaa agcgaagccg tacgggttgg
      121 agttgctttc catgttggtc agcgacgact gcagctcgcc ggccattgcg ccctccatga
      181 ggatcttgta acggcgcatg tcgttgatct tgggaacgtg ctcgagctgc accggacagt
      241 tctccatgca ggcggcgcag ttggtgcagg cccagatttc atcctcggtg acgaaatcgc
      301 cgatgagcat cttttcgtcc tcgaccgccg ccgggtcctc cgcggccatg agcatcggaa
      361 cgcggtagtc catcgccgcc ttcacgtcgt tttgtatctt cttgggagaa agcggcttct
      421 cggtgaggta ggccgggcag ttgtcctggc agcgaccgca ccgggtgcag gcatcgccat
      481 ccatgagctg cttccaggtg aagttctcca ccgcacctac gccgaagctc tccgccgtct
      541 caaactcggt ggggttgatg acgggcatcg cgaatttcgt cttcgcgctt tcgttgtcca
      601 ggttctggaa atacacgttg agcgacgaga tgaggatgtg cccgagcttg tcggagccca
      661 ccaggccaat gaagacgaag gccccgagaa cgtgcaccca ccaggtcaga tagtggaccg
      721 tctcgagcgc cgggacgctc atccaggcga acaggtaggc catcgcatag ccgaccggcg
      781 accacacctc gaaggcgggg aacgcggata tcgcgatgcg cagcgcctcg gtcatgaagc
      841 ccgtgaaaat gatcagggcg atgagcacca gcgcgaaggt gtcgatgggc ttggtgtcga
      901 gccggctggg cttcaccttg tagcgtcggt agaaggccat caccaggccg acgatgacca
      961 ccacgccgaa gagatcgccc gcgagcgacc agaggagata cggggtgccc ttcaggaact
     1021 tgatatggaa gaagagctcg gtgaagtcgt cctggatcat gatgagcagc gtcacgatga
     1081 acaggcccac gaagccgaag aacagcgccg cgtgcatgat cccggcatag gcctcacgga
     1141 cgatcttccc ctgcaggaag acgtacttga tgaccgccat gatgcgcttt tccgggaaat
     1201 cggtccgaag ctccggcttg ccgacgcgcc acaccttcac ccgctgatag atggtgtaga
     1261 tgatatagcc gaatgcgatg aacatgagaa ggtaaatcgc ccagcgcatg aatccgtgcg
     1321 gtccgcccac gttgaaaaag ctcagacggg tgatcgtttc ggccggaaga ttatacaatg
     1381 tggtaaggtc cattctcgcc cctccttact gcggatagaa tgtcgatcag atcagcgtcc
     1441 gcggggttgg gattcggccg aggccgccag ccaatcgccc gcactgccgt gtgaaaaaac
     1501 gcaatcgatg acgtgatctt tcgtaaaaac tataatgaag aaacccactg caacgaaaac
     1561 attaccgaaa gcccatgccc aattgtcaac cgaatttccc gccaccgggg gcacttttta
     1621 attgacaagc gcatcaattc cccgtcaagg attgtttccg gaaaacgcgg tgcgctcaat
     1681 caacctgaag caaacgaacc gatgactaca cccgaggcgc atgacagcac gtcagtcggc
     1741 attgtgagga cgaaacatct tcacttcgcc gaacccccga acgagctcgt gctcacctcg
     1801 ggaaacaagc tgggacccat cacccttgcc tacgaaacct acggcgcgct gaacgctgac
     1861 aaaagcaatg cgatcctcgt actgcacgcg ctgtccggca cggcccatgc cgccggctat
     1921 ttcacgcatg aggaccgcta tcccggctgg tgggacacct atatcgggcc cggcaaggcc
     1981 ttcgacaccg acaagtattt catcatcagc tcgaatgtga tcggcggctg caacggatca
     2041 accgggcccg cctcgatcaa tccggcctcc gggcgcgagt acgggatcga ctttcccctc
     2101 gtaaccatac aggacatggt gaacgcgcag tggcacctgg tcacgcacct ggggatcgac
     2161 cggctccttg ccgtggccgg cggatccatg ggcggcatgc aggcgctcca gtggaccatc
     2221 tcctatcccg acatggtgca ggcggccatc gccatcggga ctgcggcgtc gatctcggcg
     2281 cagggcatcg ccttcaacga ggtgggccgt caggccatct atcgcgaccc gcactggaac
     2341 aacggcgcct actacggcgg accgccgccc aagaacggcc tcgccctcgc ccggatgatc
     2401 gcccacatca cgtatctgag cgaagacatc atgcatgaaa agttcggccg acgcctgcaa
     2461 aacaccgaga tgttgagctt cgccttcaag gaggagttca tggtggaatc gtatctccac
     2521 cagcaaggca tcaagttcgt gaaccggttc gacgcgaact cctacctgta catcacgaaa
     2581 gcgatcgact atttcgacct caagtcgtca cacgggggca gtctcatcgc cgccttcgaa
     2641 aacgtgcgct ccgactttct cgtgatcagc ttctcctccg attggctcta cccgacgccg
     2701 ctggcaaagg agatcgtgaa ggcgctgcgc gcgaacggag cgcacgtcat cttcacggag
     2761 atcgaaacga gcaaggggca cgacgctttt ttattggaaa acgagatgct cgaacggaat
     2821 atcgccaact ttttgcgaag cgagtattcg aaacgaacca acacgaatcc cgaccggtga
     2881 cactccgaca gcgccgggcg cagcgcgcaa atatttataa aaattgattg cgccggaacc
     2941 ggcttcgcgc caatcattcc ggcgacagag tacgcacccg cgcgcgggat cgccagacga
     3001 tgccgtgcgc gcattcacga ccgccggagg cgccccacgg caccatgagc acgacgcgca
     3061 tcggttacga tctcatcatc gaagaaatac ccgaacgttc gcgggtgctc gacctgggct
     3121 gcggcgacgg cgagctgctg acggaactgg ccgacgcgaa acgcgtgaac gcgtacggcg
     3181 tcgagatctc agcggagggc gtgagccgct gcctcgagaa gggcctctac gtataccagg
     3241 gcgacatcga cgagggcctg gccgattacc gctccggctc gttcgactac gtgatcctca
     3301 accagacgct gcaggacacc aagcgcccgc tctacgtcat caccgagtcg ctgcgcatcg
     3361 gccggttcgc gatcgtcagc tttccgaacc tggggtatgc cgccaaccgt ttccagctca
     3421 tgttccgcgg ggtgatgccc aaaaaccacc tcttctcgta caactggtac gacaccccga
     3481 acatccacat catgtccata cgcaacttct ccgagctgtg ccgggagaac cgctacccga
     3541 ttcaaacgga acggcacttt tccatgcgac cgcacggctc ggtccatcgc cgaagggtgt
     3601 tcccgaacct cttcgcggaa tacggttttt tcatcttacg cggccgtgac gccgcgacac
     3661 gtccacagga gacttcatga agcaggtagc cgaatacacg aagcgggaat tcatccgcgt
     3721 cctttccgcc gacgagaaat tcgcggcgat cgaagagctc gccgcggtgt tcgccggatc
     3781 gtccgtgtgc ggcgaccttg gcgcactcgt cgaatcgctc agggagcgcg aaaagatcat
     3841 gagcacgggt atcgggtttg gcatcgccat tccgcacgcc aagatcgcgt cggtcaagga
     3901 gatcgccttc gcagtgggca tctcgccccg cggcatttcc tacgattcca tggacgggga
     3961 accggtgcac ctgatcatcc tggtcgccgc gggcgagcgc cagcacaagg actacctgcg
     4021 catgctctcg aacatcatga ccatcctcaa gaatccggcg gtcaaggagc gcatcatttc
     4081 gtcgcaatcc ccggacgagg tcctcgccat cctgaaatcg gcgccgggga agtgatccgt
     4141 cgcctcaaat tgtatttgac gcgcaccgcc agtcgcgcta gcatgagcat caatactgcg
     4201 ttcgacacag aggagggact tacatgaaac gaattgcgat agccgttttg ctttttttcg
     4261 cggtgggact cgccgcgcag cagaactacc agaaatcgac ggcgatgagc ttcaagctga
     4321 agagcatctc cgcctccaag aacggcccca gcaagatcgg catgttccga tccaagcagg
     4381 tcgtatggct caacatcgag atcatcggac tgcgcaagga cgccgacaac aacttcagct
     4441 ttcagtcgga ccttacgctc aatgacccgg acaagcaaac catcctcaac gtacccaaca
     4501 tcctgaacca gcagctcaat gcgcccgatg cgaacacggt cgtggccaac tataacatca
     4561 accttggcgg gcagatgaag cccggcaaat acgatgtgac catcgtcttc cgtgacgtga
     4621 acgccgtcac ctacagcgac ttcaaggcct cgttcttcgt ctccgagtaa tatgccgaaa
     4681 cgcgctgcgc gggcgcgtaa cgcccgcgca gcggatcatc ccccctgagc tttttacttg
     4741 acttttccct ccaccggaaa agcatcacca tcgatatcgc acaccctaga cagctccgga
     4801 gttcaccatg gccgaacgcg aatattcatt caaggacatc gaactgaagt ggcaatcgac
     4861 gtggcgcgag aagaacatct tcgccgccac gcgcgacgcc tcgaagaaaa aatattacct
     4921 cctcgagatg tacccgtatc cctccgggcg cattcacatg ggccacgtcc gcaactacac
     4981 catcggcgac ctcatggcgc gctaccgtcg catgaacggc ttcaatgtgt tccacccgat
     5041 gggctgggat tcgtttgggc ttccggccga gaacgcggcc atccagaaca acatcccgcc
     5101 gaaaacatgg accgacaaga acatcgccag catgaaggcg caattcgacc gcatggggtt
     5161 ttcctatgcg tgggagcgcg agatcatgag ccacgacccc gcgtactatc gctggaacca
     5221 gtggatgttc tgccggatgt tcgagaaggg cctcgcgtac aagaaggtcg ccccggtgaa
     5281 ctggtgcccg aaatgcaaca cggtgctggc caacgagcag gtggaagacg gcctctgctg
     5341 gcgctgcgaa tcaaccgtca cgcagaagga gctcaaccag tggttctata aaatcaccga
     5401 ttacgccgag gagctcttat cggcgcacga cgacatccgc gcgggctggc ccgagcgcgt
     5461 cctcaccatg cagaagaact ggatcggccg ctcgacgggg ctcatgatca acttcacggt
     5521 cgacgacgga acgcccttcc ccatcttcac cacgcgcccc gacacggtat acggcgtcac
     5581 cttcatggtg atcgcgccgg agcacccctt cctgaaacgc gtgaccgacc cggcggtgct
     5641 cgccttcatc gagcgcctca aggcccaatc cctggtggac cgcgtgtccg aggaaaagga
     5701 gaaggagggg atcgacaccg gaatcaaaat cgtcaacccc ttcaacggcg accgcgtccc
     5761 gctcttcgtg gggaatttcg tcctcatgga atacgggacc ggcgcgatca tggccgtgcc
     5821 cgcgcacgac acgcgcgact tcgccttcgc gaagaaatac ggcatcgcca tcaagctcgt
     5881 gatcgacaat ccggcatcgc ccatcgacgt ttccgccatg atggacgcct atgtcgacga
     5941 gggcgtatgc gtgaactccg gcccctttac gggcgtgccc aaccgcgcgg cgatggagaa
     6001 aatcgccgcc tatgccgaaa cggaaggttt cggcgcccgc gaggtgcagt atcgcattcg
     6061 cgactggctc atttcgcgcc agcgctactg gggctgcccc atcccggcca tctattgcga
     6121 cgcctgcgga acggtgatgc cgcgcgatga ggacctgccg atcacattgc ccacgcaggt
     6181 cgacttcaag ggcgactcgg tatcgccgct ggtatccatg ccgtccttcc acaataccac
     6241 ctgcccgaag tgcgggggcc ccgcgcgacg cgacacggac accatggaca ccttcgtgga
     6301 ctcatcgtgg tatttcgcca agttcacctc cgccggctcc cccggcatgt tcgacaccga
     6361 cgaggtcgac tactggatgc cggtggacca gtacatcggc ggcatcgagc acgcggtgtt
     6421 gcacctgctc tacgcgcgct tttacacgaa ggtgatggcc gacttcggac tcatcaaatt
     6481 tcgcgagccg tttacgcgcc tgctcacgca gggcatggtg atcaaggacg gcgccaagat
     6541 gagcaagtcc aagggcaacg ttgtagatcc cgacaagatc atcgaggaat acggcgccga
     6601 caccgtgcgc cttttcatgc tcttcgcctc gcccccggac aaggacctcg actggtccga
     6661 caagggcgtc gagggatgct atcgttttct cacgcgcgtg tggcggatgg tgggccggca
     6721 cggcgagcgc taccgtccgg cgccgtcgtt cgacgggatc gaactcagca aggccctcaa
     6781 aacgcttcgc ggagacctgc accggacgat caaggccgtg accaacgaca tcgaggagcg
     6841 cctgcagtac aatacggcca tcgcgcggat gatggagctg gtgaacgcgg tctaccagac
     6901 cgacgaggtc gaactggcga gcgaccatgg cgcgctcgtc gtctcggagg tgttcgatgc
     6961 gctcatcccg ctcctcgccc cctttgtgcc gcacatcgcc gacgagctgt ggcagatcct
     7021 cggccacgac gagatcctct tttcgcaccc ctggccgtct tttcgcgagg agctgacggc
     7081 gcgcgacgag gtggagatcg tcttccaggt gaacgggaag atccgctcgc gcgacctggt
     7141 tccggcggac ataccgcgcg atcaaatgga gaagctggcg ctcgagaacc ctcgcgtgaa
     7201 agagttcatc gccggcaaac aggtggtgaa ggtcgtgatc gtgccgggca agcttgtgaa
     7261 cgtggtgatc aagtaggaca atcgcacgac gcgaatcgaa tttcacgcac ccccatcccc
     7321 caaccttttc cccttcgtga tggggaagag gggctcgacc gcctcccccg aacgaagggg
     7381 gaggccggga gggggtcaat cacgcgaaat agctgtacac gaacacgccg ccggcaatga
     7441 gccgcatcgc cgcgtcgatg aacacgagac gccgcacccc gtcctcgccc gccgtttccg
     7501 cgatttccga aaccatggcc ctgatctttt ccggatacag cagaatgaac gggccggaaa
     7561 acacgactac caaccctatc gcgaaaacga cgtatccgat ggcgggaatg tccgcatgtc
     7621 gatattgcgt cagcggaaaa cccatgatga ccagcgacac gccatacagc gggaaaatac
     7681 gatgcgacac ccagcggcgc cacacctgaa acatggtgaa cggggcgatc agctccatcc
     7741 ccgccacgct gatgaggaag atgccgagac tcaagacata tacctgaaga agctgctctg
     7801 ggttcattgc catccatgta tcacgggcgc gacggtacgc gctcaccgca ctgcatcgaa
     7861 aatccgcatg aagagcgccg atcgattcag gtccatcggc ggcacatcgg ggaaatagag
     7921 caccatgcgg accgcccatg ttgcgaggag cgccacgata atcgcgatcc agaaacgatc
     7981 gcttcgataa aatcggtcaa agggccgccg atcccgaaag gcccacacca ccaacaccac
     8041 cggcgccagc gcgaagagcg gatgataccg caacgcgaga aagaactccc cgcgaacaag
     8101 gctcaggtac gcccgtgtga gaccgcagcc gggacagggc gccccgtaga tgttcttgaa
     8161 aaagcacagg gtgtcgttgc caaaaaccgc caacccgagc gcggccgccg cacagagaac
     8221 atacggcttt gcgtccctgc gctccattcc cggtgcgcgc tattgcgcct gcagtccctg
     8281 cgcggtccag atcttgttcg ccgcctcctg catgatggcc atgttcacca tgattagtcc
     8341 gaagcaggcg aggaccagat acaggatcgc attgtcctca accggctggc ccgccttctg
     8401 ctgcgcttcc atcatcaatt tcccgaactt gtatgaccaa taaatggcat agggaaaaca
     8461 caccaggcac agcaggagct ccaggccggg gttgatttcc tccttgttca gataattctt
     8521 gatctccttc gagaacttaa agatccacac cagcccgtag atcccgcacg tgataagcgt
     8581 gaacacgatg accgcggcgg gcgatcgcac ttccggtttt tccgtcatgg catcctcctc
     8641 tttctgcgct gcatgcgtgg cccgagcctg acatcaacgg cccgggcccg tcaagataaa
     8701 aacgtttatc cgcgttatcg ccaactcgac atcatagcaa gcgatgcggc cggtatcaca
     8761 taaccgtacg acatgtattc gacgcgtgca actccgctat tgtatgccgg ccttccgata
     8821 cgcgtcggct aagttcgtgt tcacatgcac cacgccggcg ttgagcgtgc ggaaggcata
     8881 gtcgtcggtg atcttcttcg aaagccggtc ggcgtcggcc tggtttgcaa gcaccgaccc
     8941 ggcgggaaca tagtggcccg ccttgatggt cacgcccatc aggacgcagc ttggttcgat
     9001 cacgcagccg tcctcgatgc gcgacttgaa caccagcacc ttcatcccga tgaagcagtc
     9061 attgccgatc gcggcgggac cgtgcacctg cacctgatgc gcgagcgata cgcggtcccc
     9121 gacatggacg gcgtatttct tcccctgcac ctccaccagg ttcttctcga ccgcatgccc
     9181 ctcgtggaag gtctccagcg cgtgaatgat cacgccgtcc tgtacattgg actcgtcgcc
     9241 cacgtgcagc ggctggccct cgtcgccccg cacggaggcg aacggcgaca cgaagacgcg
     9301 ccggccgatg ctcacgtttc cgatcaccgc cccaagcgga tgcacatacg cggtcgggtc
     9361 aatccggggc gcatgcgcct ccggcgaaaa atcagtaacg acatttttat gaatcatatc
     9421 cgtcctccgg ttcaggattc catgcgccgg cgcattcgat ccgggcaggc atcatcactg
     9481 catggcgggc gaccgttttg taaacaacat tttacggcaa aacgaaaggg gggatgatga
     9541 aaccggcgcg aggggacgca cgacgcacac cgcggatccc ctcgttgatc gtttgcggac
     9601 gcgcgctcag tagctgcgaa acagccccgc gagtttcccc aggatgtcga cggactcgag
     9661 aactatcggt cgataggcgg cattcgccgg attgagcgcg atgcggtcct tttctttttt
     9721 gaagaccttc agggtcgcct cgtcctcgat gagtgcggcc acgatgtcgc cgttcaccgc
     9781 ggtcttttgt ttttttatga tggcgatatc gccgtcattg atcccggcgt cgatcatgga
     9841 atcgcccttg acggtaagcg cgaaaaactc gccgctcctg aacatcgcgc gcggaaagca
     9901 gaggtattcg tcgacgttct cctgcgccag gatcggcgcg cccgcggcga tgcgccccac
     9961 gtgcgggatg ctcacgacgt cgggcgcttc ggtatcggcg cggtcgacca gaagctcgat
    10021 cgcgcggctc ttgttctggt tgcagcggat gaagcccttc ttctcgatgg ccttgaggtg
    10081 gtcataggcg cccttgaccg ttatctgaaa gcgctcgccg atctcacgca ccgtgggcgg
    10141 aaacccggag gtgcgaatgc tctccttgat gaatgaaaaa atgagctcct gcttgtcggt
    10201 gagtttgttt ttcatggcgc ccttccgctc gtgatggtat gtcccggaac gagacataag
    10261 ctaaacatac aataagcaaa cgaaggtttc gtgtcaagca aaaaaatatg atccgcgaaa
    10321 attcatcatt gacagccccc cgcgcgggag atacccgcac attcgagggc gacgaatcca
    10381 cgatcgccgg acacttgcac ggagccgcgc ccacgagatg acactcaccg aatgctatcg
    10441 catactcagc ctccccgcaa ccgcgaccga cgacgagatc atccgctcct acaagtaact
    10501 cgcgctccag tatcaccccg accggaaccc ggacaagacc gaatgggcga accgctccat
    10561 ggccaccatc aatgtcgcct acacgacggt gatggggcac cgcttcaagg acgcctccgg
    10621 cgcgaaggac gaggccccga cgcgcccgga gccccagcgc cgcgcccagc gacgcgaaca
    10681 gc
//
LOCUS       DAORHF010000119        10554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5554_length_10554_cov_5.412611, whole genome shotgun sequence.
ACCESSION   DAORHF010000119 DAORHF010000000
VERSION     DAORHF010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5554_length_10554_cov_5.412611"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="PKJ16_10800"
     CDS             <1..448
                     /locus_tag="PKJ16_10800"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV47519.1"
                     /translation="DLSHIYYFGTVAICQIDIESGAIRLASGGHPPLFYFDGATGNIT
                     LINNTGPLVGIIKDSRYRDIDITLNQGDALFLYTDGVTEAQPAGSHQMYGQQRLLDNF
                     QRLIDLTSADIINTLIGSLHEFTGYADQEDDITMICIKTVVERGRA"
     gene            complement(417..1232)
                     /locus_tag="PKJ16_10805"
     CDS             complement(417..1232)
                     /locus_tag="PKJ16_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47520.1"
                     /translation="MPLAENEVQQLIVKLREKYDDYARRHSKTWFNRDAFEERLQYAI
                     GKRMNLEGFILAEIKNFEEVRQKYDKKKSAKASTGEIDRILDENKQRISKYPEVKFHP
                     KADFEIARFYGALGEFTDSWLPVLSAIIEERAIKQRAYDLEEAVNVLALRRGERHPKP
                     IEDHGLLLGRPGVTELDIDRSRAAYMREAAFSLHEIADFCQELIGMRVSGWEEPLRFD
                     KLRFEEGRRRKITDNYRNHTTYGAIVRVRESALAILEDFRLTAFRRAPAPRPF"
     gene            complement(1248..1730)
                     /locus_tag="PKJ16_10810"
     CDS             complement(1248..1730)
                     /locus_tag="PKJ16_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47521.1"
                     /translation="MMNIETTVNIAAEAVSRLDRASTALGIPRSRVIVSLLRRLVRLG
                     GVEARICASVRYQRAVPGGEWACVHLCLRDDEYEWIVDMRKLFKVSVSGIVAMAIERY
                     LDDLVSGVGGSSADNYLLRNYLLAKEYVGEVICWKLYWGFPPPEALIAAGAGCAATSS
                     "
     gene            1957..2991
                     /gene="purM"
                     /locus_tag="PKJ16_10815"
     CDS             1957..2991
                     /gene="purM"
                     /locus_tag="PKJ16_10815"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018885378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HNV47522.1"
                     /translation="MSTSYRDSGVDIDKGNQFVKRIAPIVKSTFSERVITDIGGFGAL
                     FSGSFPGMADPVLVSGTDGVGTKLKIAQMMGKHDTIGIDAVAMCVNDILVSGAQPLFF
                     LDYLACGAIDVDRMTDIVKGLAEGCRLAGCSLIGGETAEHPGVMAPEEYDIAGFAVGV
                     VDRSRIINGAAIAPGDIIIGLPSSGIHSNGFSLVRKIFLEMKGYRLDATFPDLAKPLG
                     ETILAPTRIYCKSVLAAMADGVAIKGMVHITGGGFYENIPRILPDTTAVNIDKRSVPT
                     PPIFAMLQRDGSVEDREMFTTFNMGIGLMLFVEPGTADKALERLRSHGEEPIVIGEVV
                     PLGADRVIIR"
     gene            3046..3777
                     /locus_tag="PKJ16_10820"
     CDS             3046..3777
                     /locus_tag="PKJ16_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47523.1"
                     /translation="MHLSAITIDGEAFPGTDNYPFNLAILRNTKTVAFRSPVTYFIGE
                     NGSGKSTLLTAIARAAGIHIWDSPHAPLVANRHADTLHRYVSVDWRDGTTPGAFFASD
                     MFRHFSLLLDEWAKTDPGLLEYFGGSSLMAMSHGQSTMAFFESRFRIRGLYLLDEPEC
                     ALSPRRLIEFLGIIHRAAATGEAQFIIATHSPILLALPGAIILSFDSPAIREIAYEDT
                     DHVRAYRSFLADPQGYLRERGAIIE"
     gene            4051..4314
                     /locus_tag="PKJ16_10825"
     CDS             4051..4314
                     /locus_tag="PKJ16_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47524.1"
                     /translation="MKRKKLQFASQKGLLSMGELTRDFCAKILGYDFDGIFLSDESSL
                     WDFAGDSIDTYHDAIRREYGVDASSVEDGNILTILRLIRDARP"
     gene            complement(4318..5631)
                     /locus_tag="PKJ16_10830"
     CDS             complement(4318..5631)
                     /locus_tag="PKJ16_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV47525.1"
                     /translation="MGVEYAAIEPYEICSIRPPTENASLTFRLTRNCPWNRCRFCPVY
                     KFGASFSRREIAEVRADIRRAQRIDDMLFDEGFIGLRGASGWDRVPALVRKIRQAQSD
                     ALGMTGMLPERAMPSAAPSAPDAVDDPRIEWFMSWFREAPDLADSISHVYAWRAGGGE
                     TCFLGDADSLILPPAYLAEAMEAARAAFPTLRRFTVYGRTKSAVKKRSMKELAAYARA
                     GLHRVHFGLESGCDEVLALMDKGVTGEEHVAGCLKTKEAGLSCSVYVMPGLGGAALSE
                     KHAHDTAAVLAKIAPDFIRLRSLEVFPQTPLEALVREGRFVEASEERVVREIRTLVEE
                     IDCATTILSDSASNLLSVGGRLPDDRAAMLEVIDSYLALDARDKLLFSLQARLESFHG
                     QYGGITTDIVDELRPYLTDDGVDYSRASDDHLARVIRLIRGKLMP"
     gene            5821..6165
                     /locus_tag="PKJ16_10835"
     CDS             5821..6165
                     /locus_tag="PKJ16_10835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV47526.1"
                     /translation="MSADDKRKYRRVKSEFTIRIKKSAVVEDANLFSVAKSVNISGNG
                     ILFSYSMPMDIGATIQVTFLKPNTFDFFEGSAKVVRVELQRDGVYDIGVELLNLTEAD
                     EKKLNYYVTKDE"
     gene            6184..6657
                     /locus_tag="PKJ16_10840"
     CDS             6184..6657
                     /locus_tag="PKJ16_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47527.1"
                     /translation="MTDNFVVKYSINSAWGIVKEIREKVGSLLQDRQKDLVDATMMTV
                     SELIENAIKYGTPVSSSTDIDFELTITDGTISISISNGIVAMSDYENVKRHLDLIGQS
                     DNPGELYTKRLMELMENPRPGVSQLGLYRIAYEGEFSLSYRFENKILTIFAERAI"
     gene            6680..7036
                     /locus_tag="PKJ16_10845"
     CDS             6680..7036
                     /locus_tag="PKJ16_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47528.1"
                     /translation="MQDFEHGALKITIGESSGTARLAWSGKSEDREPGALLTPYLQDA
                     VREFQGKPFEVDFTKLEYMNSSTVPPIIHFIKDLESSGSKTTIFYDKDSKWQAASFKA
                     LETISTTMKHITVQGA"
     gene            7059..7787
                     /locus_tag="PKJ16_10850"
     CDS             7059..7787
                     /locus_tag="PKJ16_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47529.1"
                     /translation="MTPEERRCIDRVRRAVGRAINRYRMIAAGDRVLLALSGGKDSLA
                     LADTLHARLRHLPISYELRAAHVRVRGITDTVDTDYLAMFCGERGIALDIIDIALDPA
                     DDPDGSACFRCARARRTALFQLARDVGCPTIAFGHTMDDAVETLLMNMTFNARVSGIP
                     GRLALFGGALALVRPLILLSDAQVARYASVAGLKPSGGNCPYGAAGAREAVRALIGDL
                     GRLSPRARTNLFHATSNIDPEYLP"
     gene            7891..8196
                     /locus_tag="PKJ16_10855"
     CDS             7891..8196
                     /locus_tag="PKJ16_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47530.1"
                     /translation="MIDVLKKTVLTGIGLATLGAEKIEEYAKKIAEDSKLAESEGKKF
                     VDELLRDSEIARKNLEAKINELITTALDRMDLTSKKEVEELKKKLADLEEQLRNRAG"
     gene            8201..9910
                     /locus_tag="PKJ16_10860"
     CDS             8201..9910
                     /locus_tag="PKJ16_10860"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HNV47531.1"
                     /translation="MIGRIVRLGRGYRHLGRLREIFLVLARHGFGDFAQRLGLPRPLR
                     ARRTRGARDVVDDPTRWQRMRMVIEELGPAFIKLGQIMSNRPDLLPNDLIDELEKLQD
                     AVPPFPARDSRALIESEFGRPIESIFRRFDDIPLATASIAQVHRAELPDGTPVAVKVQ
                     RPGIERVIDVDLEIMRHLAALMEKYVEGAGYLNPVGVVEEFERVIRQEIDFHLEAIHL
                     ERFAANFSGNRTIRVPRVHKSHSTRRVLTMEYIEGTKLSGIIRDTAPGANLKLVASRG
                     ADLLLEQIFQHGFFHADPHPGNIFIMPKNVICFLDFGMMGSLPESQREQFVSIIFGAV
                     TRDARRITGALQRLTEERYIEQKHRLERRLSDLLDQYAYLPLKNIDIARLLRDLIGTL
                     IAFRLTLSPNFYLLVKSFITIEGVARMLDPDFNIVEHMEPLAKRLFRERLHPKRVARD
                     AAHAAVDLAFLLRDLPYEVRELIDQLKGGKVRIVFEHQGLESMIRRHDAISARIATAI
                     LVAALVVGSSLIVLAGVPPLWRGVPTIGVAGFVLAAVLAFGLSISMIWRGTRDRSPDT
                     SGR"
     gene            complement(9858..>10554)
                     /locus_tag="PKJ16_10865"
     CDS             complement(9858..>10554)
                     /locus_tag="PKJ16_10865"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV47532.1"
                     /translation="TETSPIINMNRPGRNRPGTVGPPVPGVTEKISEEGEVLVKGPMV
                     FSGYLDDPDEDRAAFTDDGFFRTGDIGQLDDDGNLTITSRLKDIIITSGGKNICPLVL
                     ERLFDEDELIEHICVVGDRRKYLSALVVPDFAFLSNYARTHNVRYYTNDDLVKKPEIV
                     TLYRKRIEQVCAPLAQYEQIKRFTLLPNEFSIETGELAATLKLRRAVIQEKHREIIDR
                     MYPESDLVSPARS"
BASE COUNT         2089 a         3475 c         3082 g         1908 t
ORIGIN      
        1 ggacctgtcg cacatctact atttcgggac cgtggcgatc tgccagatcg acatcgaatc
       61 cggcgccata cggctcgcgt cgggcgggca cccgccgctg ttctacttcg acggcgcgac
      121 ggggaacatc acgctcatca acaacaccgg gccgctggtt ggaatcatca aggactcgcg
      181 gtatcgcgac atcgacatca ccctgaacca gggcgacgcg ctgttcctgt ataccgacgg
      241 cgtgaccgag gcgcagccgg cggggagtca ccagatgtac ggccagcagc ggctgctgga
      301 caatttccag cggctgatcg acctcacctc cgcggacatc atcaacacgc tgattggctc
      361 gctgcacgag ttcacgggct atgccgacca ggaggacgac atcaccatga tctgcatcaa
      421 aacggtcgtg gagcgggggc gcgcctgaag gcggtgaggc ggaagtcttc gaggatcgcc
      481 agcgccgatt cgcgcacgcg aacgatggcc ccgtaggtgg tgtggttccg atagttatcg
      541 gtgatctttc gcctgcggcc ctcctcgaaa cgaagcttgt cgaaccgtaa tggctcctcc
      601 caaccggaaa cgcgcatccc gataagctcc tggcagaaat ccgcgatctc atgcagcgaa
      661 aacgcggcct cgcgcatgta ggccgcgcgg ctccggtcaa tatcgagctc ggtcactccc
      721 ggtctcccca ggagcaaccc atgatcctcg ataggcttcg gatggcgctc gccgcgccgc
      781 aacgcaagga cgttgaccgc ttcttcaaga tcataggctc gctgcttgat ggcgcgctcc
      841 tctatgattg ctgaaagcac cgggagccag ctgtctgtaa attcgcccag tgctccgtaa
      901 aacctcgcta tctcgaaatc ggcctttggg tgaaacttta cctccgggta cttgctgatc
      961 cgctgcttat tctcgtcgag gatgcggtcg atctcgccgg tagacgcctt cgcactcttc
     1021 ttcttgtcgt acttctgccg cacctcctcg aagttcttga tctcggcgag tatgaaaccc
     1081 tcgagattca tccgcttccc aatggcgtac tgcaaccgtt cttcgaaggc gtcacggttg
     1141 aaccaggtct tgctatgccg cctggcgtag tcgtcgtact tctcgcggag cttcacgatg
     1201 agctgctgga cttcgttttc tgctagcggc attatagctc ccagtatcta tgaggatgtg
     1261 gcggcgcatc ccgcacctgc tgcgatcagg gcctctgggg gagggaagcc ccagtacagc
     1321 ttccagcata ttacctcacc gacatattct tttgcaagta gataattccg aagcagatag
     1381 ttatcggcac tactaccgcc tacaccggat accagatcgt cgagatagcg ctcgatcgcc
     1441 atcgcaacaa tccccgaaac cgacacctta aacaacttcc gcatatctac aatccactcg
     1501 tactcatcat cccgcaggca cagatgaaca cacgcccatt cgccgcccgg cacggcccgc
     1561 tgatatcgca ccgacgcgca tatccgcgcc tcaaccccgc ccaagcgcac caggcggcgc
     1621 aacagactca cgatcacgcg gctgcgcgga atcccaagcg ctgttgaggc gcgatccaag
     1681 cgcgaaaccg cttccgcggc aatgttcacg gtcgtttcaa tgttcatcat tggcgtccct
     1741 cctttcgata tgacgtatca tcattaatac gcgctagaat aatgattcgt gaagttttta
     1801 ttacgaatag cagcagatca ctattttagt tttttattgt tgatttttga actcccgcaa
     1861 taagcatact gccattttcg gggttgatga ttgcctccgg catccaatcc gcatgccgca
     1921 ccagcgtaaa cacccagaac acaaaaggga catgcgatga gcacctcata ccgcgactcc
     1981 ggcgtcgata tcgacaaggg gaaccagttc gtaaaacgaa tcgcgccaat cgtcaaatcg
     2041 acgttcagcg agcgcgtcat caccgatatc ggcggcttcg gggcgctttt ctccggatcg
     2101 tttccgggaa tggccgaccc ggtcctcgtc tccggcaccg acggtgtggg caccaagctc
     2161 aagatcgcgc aaatgatggg aaaacacgac accatcggca tcgacgccgt cgcgatgtgc
     2221 gtgaacgaca tcctcgtctc gggcgcgcag ccgctctttt tcctggacta tctcgcctgc
     2281 ggggcgatcg acgtggaccg gatgaccgac atcgtgaagg gcctcgcaga gggctgccgg
     2341 ctcgcgggct gcagcctcat cggcggggaa acggcggagc atccgggagt gatggcgccc
     2401 gaggagtacg acatcgccgg gttcgcggtg ggcgtggtgg accggagccg catcatcaac
     2461 ggcgccgcga tcgctccggg tgacatcatc atcggcctcc cctcctccgg catccactcg
     2521 aacggttttt cgctcgtgcg aaaaatcttc ctcgagatga agggctaccg gctcgacgcc
     2581 acgtttcccg atcttgcgaa accgctcgga gaaacaatcc tcgcgcccac gcgcatctac
     2641 tgcaagagcg tcctcgctgc catggccgat ggcgtcgcga tcaagggcat ggtgcacatc
     2701 accggcggcg gtttctacga gaacatcccg cgcatcctgc ccgacacgac ggcggtgaac
     2761 atcgacaaac gaagcgtccc cacgccgcca atcttcgcca tgctgcagcg ggacggctcg
     2821 gtcgaggatc gagaaatgtt caccaccttc aacatgggga ttgggctgat gctgttcgtc
     2881 gaaccaggaa cagccgacaa ggcgttggag cggttgcgca gtcacggcga ggagccgata
     2941 gttatcggtg aggtcgtccc gctgggcgcc gatcgcgtca ttatacgtta acaaccaggg
     3001 cgaagcaggc ggctcaccgg cacagaaccc aaactggcac gatccatgca cctctcggca
     3061 atcaccatag acggcgaggc cttcccgggc accgataact atccgttcaa tctcgcgatt
     3121 ttacgaaaca cgaagaccgt cgcgttccga tccccggtaa cctacttcat cggcgagaac
     3181 ggctcgggaa aatcgaccct gctcaccgcg atcgcgcgag cggcgggcat acacatctgg
     3241 gacagcccgc acgctccgct ggtggcaaac cggcacgccg acaccctgca ccgatacgta
     3301 tcggtcgact ggcgcgacgg gacgacgccg ggggcctttt tcgcctccga catgttccgc
     3361 cacttctccc tcctcctcga cgaatgggcc aaaacagatc cgggactgct ggaatacttc
     3421 ggcggctcct cgctcatggc catgtcgcac ggtcagagca ccatggcgtt tttcgagagc
     3481 cgctttcgca tccgggggct ctatctgctg gacgagcccg agtgcgcgct ctcgccccgg
     3541 cgcctcatcg agtttctcgg gattatccat cgggccgcgg cgacgggcga agcgcagttc
     3601 atcatcgcga cgcactcgcc catcctgctc gcgctgcccg gcgcgatcat cctgagcttc
     3661 gactcgccgg caattcggga gatcgcctac gaggacaccg atcacgtccg cgcataccgt
     3721 tcctttctcg ccgatccgca gggatacctg cgcgagcgcg gcgcgatcat cgaatgaacg
     3781 ggacggcatg tatggtggcg catgcacgat gtcctgccgc ctctatgaat gtgcgttgat
     3841 atggattgtt tttggcgctc ctccccctaa cgaaagggga ggccgggagg gggtcgtaac
     3901 gatcggcatt tatgaaaaaa acttcaagaa tcgtgaagct tcctggggag agcatcattg
     3961 tattatcgtg tgggttgcac gatacaggtc gcttcaccat acgcgcgaca tcatcgagcg
     4021 aaacaactag gtaagcgaag aaaaaacgca atgaaacgaa aaaagctcca attcgcctcg
     4081 cagaagggac tgctctccat gggggagctc acgcgcgatt tctgcgcgaa gatactcgga
     4141 tacgatttcg acgggatctt cctctcggac gaatcgagcc tgtgggattt cgcgggcgat
     4201 tcgatcgaca cctatcacga cgcgatccgc agggaatacg gcgtcgatgc gtcctcggtc
     4261 gaggacggaa acatcctgac gatcctgcgc ctcatccgcg atgcgcggcc gtgagcccta
     4321 tggcatgagc ttcccgcgta tgagacggat cacccgcgca aggtgatcgt ccgaggcgcg
     4381 cgagtaatcg acgccatcgt ccgtgagata cggacgcagc tcgtccacga tgtcggtggt
     4441 gatgccgccg tactgcccgt gaaatgactc aagccgcgct tgcaggctaa agagcaattt
     4501 atcgcgcgca tccagcgcaa gataggaatc gatcacctcc aacatggcgg cgcgatcatc
     4561 gggcagccga ccgcccacgg acagcaggtt cgacgcgctg tcgctcagga tcgtcgttgc
     4621 gcaatcgatc tcctccacca gcgtccggat ctcgcgcacc acccgctcct ccgacgcctc
     4681 cacgaaccgc ccctcgcgca caagcgcctc aagcggcgtt tgcggaaaca cctccagcga
     4741 gcgcaggcgg atgaagtcgg gcgcgatctt cgcgaggacc gccgccgtat catgcgcgtg
     4801 cttctccgaa agagcggcgc cgcccaaccc cggcatcaca tacaccgagc acgagagccc
     4861 cgcctccttc gtcttgagac agccagccac gtgctcttca cccgtcacgc ccttgtccat
     4921 gagcgcgagc acctcgtcgc agccgctctc gagcccgaag tgcacgcggt gcagccccgc
     4981 gcgggcatag gccgcaagct ccttcatcga gcgcttcttc acggccgact tcgtgcgccc
     5041 gtacaccgtg aagcgccgca atgtcgggaa cgcggcgcgc gccgcctcca tcgcctcggc
     5101 gaggtacgcg ggcggcagga tgagcgaatc ggcgtcgccc aggaagcagg tctcgccgcc
     5161 gccggcgcgc caggcgtaca cgtgcgaaat gctgtccgcg aggtcgggcg cctcccgaaa
     5221 ccaggacatg aaccactcga ttcgcggatc atccacggcg tcgggggctg atggcgctgc
     5281 cgaaggcatc gctcgctcag gcagcatgcc ggtcatcccg agcgcgtcgg actgcgcctg
     5341 ccggatcttc cggaccagcg ccgggacccg gtcccagccg gaggcccccc gcaggccgat
     5401 gaatccttcg tcgaaaagca tgtcgtcgat ccgttgcgcg cgccggatgt ccgcgcgcac
     5461 ctccgcgatc tcccgccggc tgaaggacgc cccgaacttg tacaccggac aaaatcggca
     5521 gcggttccac gggcaattgc gggtgagccg gaaggtgagg ctcgcattct ccgtgggagg
     5581 acggatggaa cagatctcgt acggctcgat cgcggcatac tccacgccca tggaaacacc
     5641 tccaacgcag gtgtgaacag atgcccggcg cgccgggaaa tttcaataca aatacgcgtg
     5701 ctccccaccg gccaattcca catgtgaaaa atgtcttgat ttattattcg ccgacggatt
     5761 acgatggcag gcgtatgcgg gccattaggg ccgcggaaca cacacccggg gacacccgtc
     5821 atgagcgcag acgacaagcg aaaataccgg cgcgtaaaga gcgaattcac catccgcatc
     5881 aagaaaagcg cggtggtcga ggacgcgaac ttgttcagcg tcgccaaatc ggtcaacatc
     5941 agcggaaacg gcatcctgtt ctcctacagc atgcccatgg acatcggcgc cacgatccag
     6001 gtgaccttcc tcaagcccaa cacattcgat tttttcgagg gcagcgccaa ggtggtgcgg
     6061 gtggagctgc agcgcgacgg ggtgtacgac atcggcgtcg agctgctcaa tcttaccgag
     6121 gccgatgaga agaagctcaa ttactacgtc acgaaggacg agtaggaaac cgcggaacca
     6181 tcgatgaccg acaatttcgt cgtcaaatac tccatcaatt cggcctgggg catcgtcaag
     6241 gagatccgcg aaaaggtggg atcgctcctc caggaccgcc agaaggacct ggtggacgcc
     6301 acgatgatga ccgtctccga gctcatcgaa aacgcgatca agtacggcac gcccgtatcc
     6361 agcagcaccg acatcgactt cgagctcacc atcacggacg ggacgatctc catcagcatc
     6421 tccaacggga tcgtcgccat gtccgactac gaaaacgtga agcggcatct cgacctcatc
     6481 ggacaaagcg acaacccggg cgagctctac accaagcgtc tcatggagct catggagaac
     6541 cccaggccgg gcgtgagcca gctcgggctc taccgcatcg cctacgaggg ggagttctcc
     6601 ctttcgtatc gcttcgaaaa caaaatcttg acgattttcg ccgagcgcgc tatctaggct
     6661 tatcaatcag gagaacacca tgcaagattt cgaacatggc gcattgaaaa tcaccatcgg
     6721 cgaaagcagc gggaccgcgc gcctcgcatg gtccggcaag agcgaggacc gcgagccggg
     6781 cgcgctcctg actccctatc tccaggacgc ggtccgcgaa ttccagggaa agccgttcga
     6841 ggtcgacttc acgaagctcg agtacatgaa ttcgtcgacg gtgccgccca tcatccactt
     6901 catcaaggac ctggagagca gcggatcgaa aacgacgatc ttctacgaca aggactcaaa
     6961 atggcaggcg gcctccttca aggcgctcga gaccatctcc accaccatga agcacatcac
     7021 cgtccagggc gcgtgacgcc gcattctgat cccgccgcat gacccccgag gaacgccgct
     7081 gtatcgaccg ggtgcggcgt gccgtcggca gggccatcaa ccgctatcgc atgatcgccg
     7141 cgggcgaccg cgtgctcctt gcgctctccg ggggaaagga ctcgttggcg cttgcggaca
     7201 cgctccatgc ccgcttgcgg catctgccca tctcctacga actgcgcgcg gcacatgtgc
     7261 gcgtccgcgg catcacggat acggtcgaca ccgactatct cgctatgttc tgcggcgagc
     7321 gcgggatcgc gctcgacatc atcgacatcg ccctggaccc ggccgacgat ccggacggct
     7381 ccgcgtgttt ccggtgcgcc cgcgctcggc gcacggcgct ctttcaactg gcgcgagacg
     7441 tcgggtgccc gaccatcgcc ttcgggcaca ccatggacga tgcggtggag acgctcctca
     7501 tgaacatgac cttcaacgca cgcgtgagcg gcataccggg gaggctcgcg ctctttggcg
     7561 gagccctggc gctcgtcaga ccgctcatcc tgctctccga tgcgcaggtc gcgcgctatg
     7621 cgtccgtcgc ggggctcaaa ccgtcgggcg ggaactgccc gtacggcgcc gccggcgcgc
     7681 gggaggcggt gcgcgccctc atcggcgacc tcggcaggct ctctcccagg gcgcggacca
     7741 acctgttcca cgcgacctcg aacatcgacc cggagtatct gccctaggcg caaaatattt
     7801 cttgccgcac cgaggcaccc tgtggtatcc tctcggagtc atacacggcg gctcgccgcg
     7861 cgtatcacca cacaaaagga ggacaccgca atgatcgatg tattgaagaa gaccgtactc
     7921 accggcattg gcctggcgac gctgggcgcc gagaaaatcg aggaatacgc gaaaaagatc
     7981 gcggaggaca gcaagctcgc cgagtccgag ggaaagaagt tcgtcgacga gcttctgcgc
     8041 gattccgaga tcgcgcggaa aaacctcgag gcgaagatca acgagctcat cacgacggcc
     8101 ctggaccgca tggacctcac ctcgaagaag gaggtggagg agctcaagaa gaagctcgcc
     8161 gatctcgagg agcagctcag gaaccgcgcg ggctgacgcc gtgatcggac gcatcgtccg
     8221 cctggggcgc ggctaccggc acctcggccg cctccgggag attttcctgg tcctggcgcg
     8281 ccacggcttc ggtgatttcg cacaacgcct gggcctgccg cggccgcttc gcgcgcgccg
     8341 gacgcgcggc gcgcgcgacg tcgtcgacga ccccacgcgc tggcagcgca tgcgcatggt
     8401 gatcgaggag ctgggccccg ccttcatcaa gctcgggcag atcatgagca accggcccga
     8461 ccttctgccg aatgatctca tcgacgagct cgagaaactg caggacgccg ttccgccgtt
     8521 cccggcgcgc gattcgcgcg ccctcatcga atctgagttc ggcaggccca tcgaaagcat
     8581 cttccgccgg ttcgacgaca tccccctcgc gaccgcctcg atcgcgcagg tgcaccgcgc
     8641 cgagcttccc gacggaacgc ccgtggccgt gaaggtccag cgccccggta tcgagcgcgt
     8701 catcgacgtc gacctcgaga tcatgcggca ccttgccgcc ctcatggaga aatacgtgga
     8761 gggcgcgggt tacctgaatc ccgtgggcgt ggtggaggag ttcgagcgcg tcatccggca
     8821 ggagatcgat ttccacctgg aggcgatcca cctcgagcgt ttcgcggcga acttcagcgg
     8881 caatcggacc attcgcgtcc cgcgcgtcca caaaagccac tccacccggc gcgttctcac
     8941 tatggaatac atcgagggga ccaagctctc cggcatcatc agggacaccg cgccgggcgc
     9001 caacctgaag ctcgtcgcca gccgcggggc cgaccttctc ctggagcaga tcttccagca
     9061 cgggtttttt cacgccgatc cgcatcccgg caacatcttc atcatgccaa agaacgtcat
     9121 ctgcttcctc gatttcggta tgatgggctc gctccccgag tcgcagcgcg agcagttcgt
     9181 gagcatcatc ttcggcgcgg tcacgcgcga cgcgcggcgc atcaccggcg cgctccagcg
     9241 cctcaccgag gagcgctaca tcgagcagaa gcaccgcctg gagcggcgcc tgtccgacct
     9301 cctggaccag tacgcctacc tgccgctcaa gaacatcgac atcgcccgac tgctgcgcga
     9361 cctcatcggc acgctcatcg cgttccggct cacgctctcg ccgaacttct acctgctggt
     9421 gaagtccttc atcaccatcg agggcgtggc ccgcatgctc gatcccgact tcaacatcgt
     9481 ggagcacatg gagccgctgg cgaagcggct cttccgcgag cgcctccacc cgaagcgcgt
     9541 tgcgcgcgac gccgcgcacg cggcggtcga tctcgccttc ctgctgcgcg acctccccta
     9601 cgaggtccgc gagctcatcg accagctcaa gggcggcaag gtgcgcatcg tcttcgagca
     9661 ccaggggctc gagtcgatga tccggcgcca cgacgcgatc agcgcgcgca tcgcgacggc
     9721 catcctggtc gccgcgctcg tggtgggatc gtcgctcatc gtgctggcgg gcgtgccgcc
     9781 cctgtggcgc ggcgtcccga cgatcggcgt cgccgggttc gtcctcgcgg cggtcctggc
     9841 gttcgggctg tcgatttcta tgatctggcg ggggacacga gatcgctctc cggatacatc
     9901 cggtcgatga tctcgcgatg tttttcctga atgaccgcgc gccgcagctt gagggtcgcg
     9961 gcgagctccc ccgtttcgat ggagaactcg ttcggcagca gcgtgaagcg cttgatctgc
    10021 tcgtactgcg caagcggcgc gcacacctgc tcgatccgct tccggtacag cgtcacgatc
    10081 tccggctttt tcaccaggtc gtcgttcgtg taatagcgca cattgtgcgt tcgcgcataa
    10141 ttcgacagga aggcgaaatc ggggacgaca agcgccgaga ggtacttccg ccggtcgccc
    10201 accacgcaga tgtgctcgat gagctcgtcc tcgtcgaaga gcctctcgag caccagaggg
    10261 catatgttct tcccgcccga ggtgatgatg atatccttga gccggctcgt gatggtgaga
    10321 ttgccgtcgt catcgagctg tccgatatcg cccgtgcgga aaaacccgtc atcggtgaac
    10381 gccgcgcggt cctcgtccgg atcgtcgagg tagcccgaaa acaccatcgg ccccttcacc
    10441 aggacctcgc cctcctccga gatcttctcg gtcacgccgg gtacgggcgg gcccaccgtg
    10501 ccggggcggt tccgcccggg gcggttcatg ttgatgatgg gcgaggtctc ggta
//
LOCUS       DAORHF010000120        10538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5567_length_10538_cov_4.470762, whole genome shotgun sequence.
ACCESSION   DAORHF010000120 DAORHF010000000
VERSION     DAORHF010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5567_length_10538_cov_4.470762"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1457
                     /locus_tag="PKJ16_10870"
     CDS             21..1457
                     /locus_tag="PKJ16_10870"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HNV47533.1"
                     /translation="MKRILFTISLIILPASLAFANYGDTYGISAKAVGMGNAVTSIVD
                     DWSSTWYNMSGLGKVPPKAELKETKTKVSLLYKTLTGEKKQKTISEKSEDGGEAHKIN
                     NNELAVTYLYTIPNLEVTTDVAQETKNEKLQFGAFVLGLVIDMNTFIRMPFDLNARLG
                     VLFAIQDDLGLGKVTDDDPRTHYFMRYGRRIQRFITNLGVGIEVWKNHMYIGGGISVF
                     AGGKGSVQIQKVQITQEDQNPPTRTKLDLQPSSAPLAGLSFKWDKLGPGDFNFGFCYR
                     DEIYLKIDPFDTQATTDLAGVDMKMSIAIFDFYTPKSFTGGLSYAIQDLWIFKELVFS
                     VDAELQQWSRYNDLAKNYLKRWEDTFEVSYPQFKDIIIYKAGFEQTVLPWLKIREGYM
                     MQPAFTPEQNGNSNYLDNDKHIISLGAGFIIPDVIVLKVPVEVDIAYQYQMLQERSAT
                     KDTTNPYNPSYKYGGSVHTFFGSIRWRY"
     gene            1620..3416
                     /gene="lepA"
                     /locus_tag="PKJ16_10875"
     CDS             1620..3416
                     /gene="lepA"
                     /locus_tag="PKJ16_10875"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HNV47534.1"
                     /translation="MNSAHIRNFSIIAHIDHGKSTLADRIIEKCNLLDSRNHMDQILD
                     TMDLERERGITIKSNAITLNYRSNAGEEFVFNLIDTPGHVDFTYEVSRALAACEGVLL
                     VVDASQGVEAQTLANLYLAIDNDLEIIPVINKIDMPSADVEATRKSIQKTLGLDPAHA
                     ALTSAREGIGIDDLMEKIIEYIPPPTGSADGPLQALIFDSFFDQYVGAVIKVRIVQGS
                     VRKDEEIVLISSGRRYTVTEVGVFRLEREKRESLRPGEVGYIIAGIKAVADTKIGDTI
                     TKTANPTSEPLKGFKDVKPMVFSGLYPMYSDEYEELKDALAKLRLNDASLIFEPDNSY
                     ALGFGFRCGYLGLLHMEIVQERLEREFNLSLVTTAPSVEYHIKLTNGEELVIDNPVKL
                     PDPGRIETINEPFVESTIITPSQFVGNIMAIVNECRGIHKNMEYIDETRVQLHYDMPL
                     SEIVFNFYDKLKSCSRGYASFDYEIKDYRPSNMVKVDILVNAEPVDALSFIVHSDKAY
                     YRGKEIIEKLKDIIPRHQFKIPLQAAIGAKIIARENISALRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRMKQIGNVDIPQEAFMTILKID"
     gene            3505..4101
                     /locus_tag="PKJ16_10880"
     CDS             3505..4101
                     /locus_tag="PKJ16_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV47535.1"
                     /translation="MTQLRSSHGWDHVQRVTCMAERIAEAEGADPHIVRCAAILHDIA
                     RADEDASNGAMCHAQRGSELAREFLSSLHVDPSNIARIAACIAEHRYRGGDVPDSIES
                     RVLFDADKLDSIGAIGIGRAFLFSGEIGARLHNDDADIDATRAYTEEDTAYREFVVKL
                     RHIRAAMLTDTGRAIAEERHRFMEDFFERLQREAHGHA"
     gene            complement(4111..6666)
                     /gene="glgP"
                     /locus_tag="PKJ16_10885"
     CDS             complement(4111..6666)
                     /gene="glgP"
                     /locus_tag="PKJ16_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HNV47536.1"
                     /translation="MATIKRFRVIPSLPESLQPLMKIAKNLWWVWNFEAIELFRRLNV
                     DLWRELHHNPVAFLGSISQKELEEVAKSESFIAHLNRVTEELEWHMTKRSWFEENYPE
                     SRDMQVAYFSAEFGLHECLPIYSGGLGVLAGDHLKSSSEIGVPLCGVGLLYRLGYFHQ
                     YLNIDGWQQEAFPETDFFNIPISIMKDEQGQPISISVEFPGRVVHAHIWEVRIGRISL
                     YLLDTNIDQNAIEDRAITDQLYGGDLEMRIKQELLLGVGGVRALKRLGKRITVYHMNE
                     GHSAFLAVERIRDVMMTERLAFREALEFVTAGNVFTTHTPVPAGNDRFPGELIEKYLS
                     HYYAPLGLAKEAFMGLGRENPTDKNETFCMTVLALRTAASCNGVSKLHGSVSRAMWRN
                     IWPSLPVHEVPIDHITNGVQILSWTSDEMMRLLNRYLGPRWIDNPVDRDIWNNVDSIP
                     DSELWRTRERLRERLIVFARRVLKSQLATRGLPKNEVDRSDSVLDADALTIGFARRFA
                     TYKRGTLIMKNMDRLEKILLNRERPVQIIFAGKAHPHDNLGKELIKHIVNLVRDERFR
                     NKIVFLEDYDINIARNMVQGVDVWLNTPIRPLEASGTSGMKVVPNGALNLSILDGWWN
                     EGYNGKNGWAIGRGEEYLDHGYQDEVESLSLYNILEKDLIPSFYSRGADGLPHEWVTK
                     IKESMKTLCPQFNTNRMVRDYAEKFYIPAHHHERAFSENEYRVSRDLAKWKSAVVAGW
                     NKVSIRDLIYGEEREIALGSRLKVRAQVDLGGLAPQDVQVELYFGALNQDGEIFEGAS
                     LPMFPVEDHGGGQHAYEGHMLCLKSGQFGFTVRAIPMHDNLVRKFEPRLIAWA"
     gene            complement(6699..7625)
                     /gene="trxB"
                     /locus_tag="PKJ16_10890"
     CDS             complement(6699..7625)
                     /gene="trxB"
                     /locus_tag="PKJ16_10890"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HNV47537.1"
                     /translation="MKDLLIIGAGPAGLTAAIYAVRAGIECAILEKFSPGGQVVNTYE
                     VENYPGFENPIPGWELMSAMEAQARRLGVPIESVDVAGIRKDDAADSFIVTTADGGEH
                     AARAVILATGASFKKVGVPGEVEFTGRGVSYCATCDGAFYKGRTVAVIGGGNTALEEA
                     LFLTRFATRVYLIHRRDEFRGAKILQDRVLSNDRITPVYDTVVGSINGATSVESVTLI
                     NKKTNVQSEQHVDGVFVFVGYEPNTAYCPAELLNPAGEVIVNRDMKTRIDGLFAAGDL
                     REGSIRQIVAACGDAAVAAVHAYAYLAEREHR"
     gene            7752..>7874
                     /locus_tag="PKJ16_10895"
     CDS             7752..>7874
                     /locus_tag="PKJ16_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47538.1"
                     /translation="MSDRIQYQNGTVTVPDKPVVLFIEGDGTGPDIWRATRAVVD"
     assembly_gap    7875..7884
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7885..9042
                     /gene="icd"
                     /locus_tag="PKJ16_10900"
     CDS             <7885..9042
                     /gene="icd"
                     /locus_tag="PKJ16_10900"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HNV47539.1"
                     /translation="IICFIEGDGIGPDIWRATRHVLDAAVASAYGGSRAITWREVLAG
                     EKAFNTTGNWLPDETLDAIKEYKVAIKGPLTTPVGGGIRSINVALRQVLNLYACIRPV
                     RYLKGVPSPVVRPDAVNMIIFRENMEDLYAGIEWKEGSEEAKRIIDFIRSELKKDIRP
                     DSGIGIKPISETNTKNLVRSAITYAIKNNRSSITLVHKGNIMKFTEGAFREWGYELAA
                     KEFGDHTVTEDELWSKFNGKCPQGKIVIKDRIADAMFQQVILRPDEYDVIATPNLNGD
                     YLSDAIAAQIGGLGMAPGANIGDTSAVFEATHGTAPKYANQDKVNPGSLILSGEMMLR
                     FMGWTEAADRIITALEKTIQQKKVTYDLERQMSGATLLTCSAFGRAVAENM"
     gene            complement(9052..9459)
                     /locus_tag="PKJ16_10905"
     CDS             complement(9052..9459)
                     /locus_tag="PKJ16_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47540.1"
                     /translation="MAHWIALYGRFRGSIVAQLPYLNAFYFLEIIYLMVSLNILYGNR
                     VALAVAMTLSLIVAVQMGLLFFRHPMSRYVQLGLMELHAAYSLPLCFNLATGAFEYHA
                     LDAGLIVARFFVACLEIVGIVALTDDAVRAEFE"
     gene            complement(9464..10207)
                     /locus_tag="PKJ16_10910"
     CDS             complement(9464..10207)
                     /locus_tag="PKJ16_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HNV47541.1"
                     /translation="MMDHVSFDRLSAYADNTPLSVADKTSIERHLAGCASCRDELARI
                     RKMLQMLSSLKGLGLVDSDRFVRNTMRRISDGGHVVHRVSKSRAVRSAATAAVAASVV
                     IAGTIFATMGGFFNTTGPTTVARNEDPRAVTVPVSYAHNRISRAMNVFRSDVEKDATV
                     SLLERHRAKIIAVTDSYVEGRVSASEFNRMLNTFYHLGGRRPYMVSSDMDGFKTVRFL
                     VGEPAQREPQTSVPAMPESDIHVLVNFHR"
     gene            complement(10204..>10538)
                     /locus_tag="PKJ16_10915"
     CDS             complement(10204..>10538)
                     /locus_tag="PKJ16_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HNV47542.1"
                     /translation="YCKNHQKKSRRQASVSINRPDDTEFEMQIHDERVDIEAGVIDRD
                     SLRIVKEELFKLPDDYREILVLRDIEGYSYNDISSMLSINLANVKVRIHRGREMLKKR
                     LSERGLLS"
BASE COUNT         2328 a         3246 c         2927 g         2027 t
ORIGIN      
        1 aataaaccat gaggaacctt atgaaacgta tacttttcac catatccctc atcatactac
       61 cggcgtcttt ggcattcgca aactatggcg acacctatgg cataagtgcc aaggcggttg
      121 gcatgggcaa tgccgtcact tcgatcgtcg acgactggtc ctcaacctgg tacaacatgt
      181 ccggcctggg caaggttccc ccgaaggccg aacttaagga aacgaaaaca aaggtctcgc
      241 tcctttacaa aacgttgacg ggcgagaaaa agcagaaaac gataagcgag aaatccgagg
      301 acggcggaga agcccacaag atcaacaaca acgagcttgc ggtcacttat ctgtacacga
      361 tccccaacct cgaggttacc accgacgtcg cccaggagac gaaaaacgag aagctgcagt
      421 tcggcgcctt cgtcctcgga ctcgtcatcg acatgaatac cttcattcgc atgccgttcg
      481 acctgaacgc gcggctcggc gtgctcttcg cgatccagga cgacctcggc ctcggcaagg
      541 tgaccgacga cgatccccgc acccactatt tcatgcgata cggccggcgg atccaacggt
      601 tcatcaccaa tctcggcgtg ggaatcgaag tgtggaaaaa ccacatgtat atcggcggcg
      661 ggatatcggt ttttgccggc ggcaaggggt ccgtgcaaat tcaaaaagtc cagatcacgc
      721 aggaagacca gaacccgccc acgagaacca agctcgacct gcagccctca agcgcgccgc
      781 tcgcaggact cagcttcaaa tgggacaagc tgggcccggg tgatttcaac ttcgggttct
      841 gctaccggga cgagatatat ctcaagattg acccgttcga cacgcaggcg accaccgacc
      901 tcgccggcgt ggacatgaag atgagcatcg ccattttcga tttctacact ccgaaatcgt
      961 tcaccggcgg actctcctac gccatccagg acctgtggat attcaaggaa ctcgtcttct
     1021 ccgtcgacgc cgaattgcag caatggtcgc gatataacga cctcgcgaag aactacctga
     1081 agcgctggga agacacgttt gaggtcagct atccgcaatt caaggacatc atcatataca
     1141 aggccggctt cgagcaaacc gtattgccgt ggctgaaaat tcgcgaaggc tacatgatgc
     1201 aaccggcctt tacgccggaa cagaacggca acagcaacta cctcgacaac gacaagcaca
     1261 tcatatccct gggtgcggga ttcatcatcc ccgatgtcat cgtccttaag gtccccgtgg
     1321 aggtcgatat cgcgtaccaa taccaaatgc ttcaggagcg atcggcgacc aaggacacca
     1381 cgaatccgta caacccgtcg tataaatacg gcggaagcgt ccataccttt ttcggatcga
     1441 tccgatggag atactgatac tcaacctcat cgcctgcgtc acgctgtgcg ttgtcatgta
     1501 cctttccgtt tgccgggcct gatccggcgg ggccggtcgt ttttgtgaca taaaaaaagc
     1561 ttgcggaaaa accgcgctgt ccgaccatct ccccaattac gtctcctgaa cgatacggca
     1621 tgaacagcgc ccacatacgg aatttctcca tcatcgcgca catcgatcac ggcaagtcca
     1681 cgctcgccga tcgcatcatc gagaagtgca accttctcga cagccgcaac cacatggacc
     1741 aaatactcga cacgatggac ctcgaacgcg agcgcggcat caccatcaag tcgaacgcga
     1801 tcacgctgaa ttaccgctcg aacgccgggg aggagttcgt cttcaatctc atcgacacac
     1861 cggggcacgt cgatttcacc tacgaggtct cccgtgcgct ggccgcctgc gagggcgtat
     1921 tgctggtcgt tgacgcgagc cagggcgtcg aggcgcaaac gctggcgaac ctctacctcg
     1981 cgatcgacaa cgacctcgag atcattccgg tcattaacaa gatcgacatg cccagcgccg
     2041 atgtcgaggc cacccggaaa agcatccaga agaccctcgg gctcgatccc gcgcacgccg
     2101 cgctcacctc tgcgcgcgag ggcatcggca tcgacgatct catggaaaag atcatcgaat
     2161 acattccgcc gcccactgga tcggcggacg gcccgctgca agcgctcatc ttcgattcgt
     2221 ttttcgacca gtacgtcggc gcagttatca aggtgcgcat cgtccagggg agcgttcgca
     2281 aagacgagga gatcgtgctg atctcctcgg gacgtcgata tacggtcacc gaggtgggcg
     2341 tgttccgcct cgaacgcgaa aagcgcgaat ccctccgccc cggggaggtg ggatatatca
     2401 tcgccggcat caaggcggtg gccgacacca agatcggcga tacgatcacg aaaaccgcca
     2461 acccaacgtc cgagcccctc aagggcttca aagacgtgaa gcccatggtg ttttccggcc
     2521 tgtacccgat gtacagcgat gaatacgagg agctcaagga cgcgctcgcc aagctccgct
     2581 tgaacgacgc ctcgctcatc ttcgagcccg acaactccta cgcgctcgga ttcgggtttc
     2641 gctgcggcta cttgggcctc ctgcacatgg agatcgtcca ggagcgcctt gaacgcgagt
     2701 tcaacctgtc gctcgtcacc accgcgccga gcgtcgagta ccatatcaaa ctcaccaacg
     2761 gcgaggagct cgtcatcgac aacccggtga agctgcccga tcccggccgc atcgaaacga
     2821 tcaacgagcc tttcgtcgag tccaccatca tcaccccctc gcaattcgtg gggaacatta
     2881 tggccatcgt caacgagtgc cggggcatac acaagaacat ggagtacatc gacgaaacgc
     2941 gcgtccagct acactacgac atgccgctct ccgagattgt attcaacttc tacgacaagc
     3001 tcaagtcctg ctcgcgcggg tatgcgtcct tcgattacga gataaaggac taccgccctt
     3061 cgaacatggt caaggtggac atcctggtca atgccgagcc ggtggacgcc ctgtcgttca
     3121 tcgtccattc cgataaggcc tattaccgcg gcaaggaaat catcgagaag ctcaaggaca
     3181 ttatcccccg gcaccagttc aagatcccgc tgcaggcggc gatcggcgcg aagatcatcg
     3241 cgcgcgaaaa catatcggcg ctgcgaaaga acgtcacggc caaatgctac ggcggcgaca
     3301 tcacccgcaa gcgcaagctg ctcgaaaagc aaaaagaggg caagaagcgc atgaaacaga
     3361 tcgggaacgt ggacatcccg caagaggcgt tcatgaccat cctcaagatc gactgacgtc
     3421 cccgccccat ccgcgtatcg caccatggca acgaaaatag acgcccggat atttcccacc
     3481 gcaatcgaat atgcaaaacg gcgcatgacg cagcttcgct cgagtcacgg ctgggaccat
     3541 gtccagcgcg tgacctgcat ggcggaacgc attgcggagg ccgagggcgc cgatccgcac
     3601 atcgtccgat gtgcggcgat cctgcacgac atcgcccgcg ccgacgaaga tgcgagcaat
     3661 ggcgcgatgt gccatgccca acgcgggagc gaactggccc gtgagttcct ttcgagtttg
     3721 catgttgatc ccagcaacat cgcgaggatc gccgcatgca tcgccgagca tcgctatcgc
     3781 gggggggacg tgccggattc gatcgagtcg cgggttctgt tcgatgcgga caagctcgat
     3841 tcgatcggcg ccatcggcat cggtcgggcc ttcctgtttt ccggtgaaat cggcgcccgg
     3901 ctgcacaatg acgacgccga tatcgacgcg acgcgcgcct acaccgagga ggatacggcg
     3961 taccgcgaat tcgtcgtcaa gctccggcat atccgggcgg cgatgctcac ggacacgggc
     4021 cgggcgatcg ccgaagagcg ccatcgcttc atggaggatt tcttcgaacg cctgcagcgc
     4081 gaggcgcacg gccacgcctg atccgcccgt ctatgcccag gcaatgagcc gtggctcgaa
     4141 cttgcgcacc aaattgtcat gcatcgggat ggcgcgaacg gtgaaaccga actgacccga
     4201 cttgaggcac agcatgtggc cctcataagc gtgttggccg cccccgtggt cttcgacagg
     4261 aaacatcggg agcgaggccc cttcgaagat ttccccgtct tggttgagcg cgccgaagta
     4321 cagctcgacc tgaacgtcct gcggcgccaa tccccccaaa tcgacctgcg cgcgcacctt
     4381 gaggcgcgag cccagcgcga tctcccgctc ttcaccgtag atgaggtcgc ggatcgacac
     4441 cttgttccag cccgcgacga ctgcgctctt ccacttcgcc aggtcgcgcg aaacgcggta
     4501 ttcgttttcg ctgaacgcac gctcgtgatg atgcgcggga atgtaaaact tttccgcgta
     4561 atcccgcacc atgcgattgg tgttgaattg cgggcagagc gttttcatcg attccttgat
     4621 cttcgtgacc cactcgtgcg ggagcccgtc ggcgccgcga ctgtagaacg acgggatgag
     4681 gtccttctcg agaatattgt acagcgacag gctctccacc tcgtcctgat agccgtgatc
     4741 cagatactcc tctccccgac cgatcgccca tccgtttttg ccgttgtacc cctcgttcca
     4801 ccaaccgtcg aggatgctga gattgagcgc gccgttggga accaccttca tgccgctggt
     4861 tcccgacgcc tcgagcggac gaatgggcgt gttaagccac acatcgaccc cctgcaccat
     4921 gtttcgcgcg atgttgatat catagtcttc cagaaacacg atcttgttcc gaaaccgctc
     4981 gtcgcgcacc agattgacga tgtgtttgat aagctccttg cccagattgt cgtgcggatg
     5041 ggccttgccg gcgaaaatga tctgcaccgg ccgctcgcgg tttaacaaga tcttctcgag
     5101 gcggtccatg ttcttcatga taagcgtacc gcgcttgtag gtcgcgaatc gtcgcgcgaa
     5161 gccgatggtg agcgcgtccg catcgagcac ggaatcggag cggtccactt cgtttttcgg
     5221 gagccctcgc gtcgccagtt ggctctttag cacgcgtcgt gcgaacacga tcagccgctc
     5281 gcgaagacgc tcgcgcgtgc gccagagctc cgagtcggga attgaatcga cgttgttcca
     5341 tatgtcgcgg tcgaccggat tgtcgatcca gcgcggcccc aggtagcggt tcagaaggcg
     5401 catcatctcg tccgaggtcc aggagagtat ctgcacgccg ttggtgatgt ggtcgatggg
     5461 cacctcgtgc acgggcagcg acggccagat gtttctccac atggcgcgcg aaaccgagcc
     5521 atgcagtttc gaaacgccgt tgcacgaggc cgcggtccga agggccagca cggtcatgca
     5581 aaaggtctcg tttttatccg ttgggttttc cctgccaagt cccatgaacg cctccttggc
     5641 gaggccaagc ggcgcgtaat agtgcgacag atatttttcg atgagctcgc cgggaaaccg
     5701 gtcgtttccg gccggtaccg gcgtgtgggt ggtaaacacg tttccggcgg tcacaaactc
     5761 aagcgcctcc cggaacgcga gccgctccgt catcatcacg tcgcggatcc gctcgacggc
     5821 gaggaaggcc gaatgaccct cgttcatgtg atacacggtg atgcgcttgc ccaaacgctt
     5881 gagcgcgcga acgccgccca cacccagcag cagctcctgc ttgatgcgca tctcgagatc
     5941 gccgccatag agctgatcgg tgatcgcacg gtcctcgatc gcgttttggt caatattagt
     6001 atcaagcaga tagaggctga tccggccgat gcgcacctcc cagatgtgcg catgtacgac
     6061 gcgcccggga aattcgacgg agatgctgat cggttggccc tgttcgtcct tcatgatact
     6121 gataggaatg ttgaaaaagt ccgtttccgg gaacgcctcc tgctgccagc cgtcgatgtt
     6181 aagatactga tggaaatacc caagccgata gagcaatccc acgccgcaga gcggcacccc
     6241 gatctcgctg gatgacttca ggtggtcgcc cgctaggacc cccaggccgc cggaataaat
     6301 cgggaggcat tcgtgcagac caaattccgc ggaaaaatac gccacctgca tatcgcgcga
     6361 ttctggataa ttctcctcga accaggaccg cttggtcatg tgccattcga gctcctcggt
     6421 cacgcggttg aggtgcgcga tgaaactttc ggacttcgcg acttcctcga gctccttctg
     6481 tgatatggaa ccgaggaatg ccactggatt gtggtgcagc tcgcgccaca aatccacgtt
     6541 gaggcgtctg aacagctcga tcgcctcgaa gttccacacc caccacaggt tttttgcgat
     6601 cttcatgagc ggctgaagac tttcggggag cgaaggtata accctaaaac gcttgatagt
     6661 tgccatgctt tctcctggaa ttatgtatat gggtggtgtt agcggtgctc acgttcggca
     6721 agatacgcat acgcgtgtac ggcggcaacc gccgcatcgc cgcacgcggc gacgatctgc
     6781 cggatcgacc cctcgcggag atccccggcg gcgaacagac cgtcgatacg cgttttcatg
     6841 tcgcgattga cgatcacctc gcccgccgga ttcaagagct ccgcgggaca atacgccgtg
     6901 ttcggctcgt agcccacaaa cacgaacacg ccatcaacgt gctgctcgga ctgcacgttc
     6961 gtttttttgt tgatgagggt cacggattcg accgacgtcg cgccattgat ggatccgacg
     7021 acggtatcgt acaccggagt gatgcgatcg ttcgaaagaa cgcggtcttg caggatcttc
     7081 gcaccgcgaa actcgtcccg acgatgaatg agatacacac gggtcgcgaa ccgagtgagg
     7141 aagagcgcct cctcgagggc cgtgttcccg ccgccgatca ccgcaacggt acgccccttg
     7201 tagaaggcac cgtcgcaggt cgcgcagtat gatacgccgc gcccggtaaa ctcgacctcg
     7261 ccggggacgc cgaccttctt gaacgacgcg ccggtcgcca aaatgaccgc gcgcgcggcg
     7321 tgttccccgc catccgccgt ggtcacgatg aaggaatccg cagcatcgtc ctttcgtatg
     7381 ccggcgacgt cgacgctttc gatcggaacc cccaatcggc gcgcctgggc ctccatggcg
     7441 ctcataagct cccagccggg aatcggattt tcaaaccccg ggtagttctc gacctcatag
     7501 gtgttgacta cctgcccgcc cggggaaaac ttctcgagga tggcgcattc gataccggcg
     7561 cgcacggcat atatcgccgc cgtgagaccc gccgggcccg ccccgatgat caataggtcc
     7621 ttcatgattc ttcacgcccg gcaaaacgat tgttctgatt cttcatatca tcaacattaa
     7681 aaaatattga aatattagca ataatatttt tttatggcga acccccgaac attttcccac
     7741 aggagaccca aatgtcagat cgcatacagt atcaaaatgg aaccgtaacc gtaccggaca
     7801 agccggttgt tcttttcata gagggagatg gcaccggccc cgatatctgg agagcgaccc
     7861 gtgcagtggt cgatnnnnnn nnnnattata tgttttattg aaggggacgg aatagggccc
     7921 gatatctggc gcgccacacg gcacgttctc gacgccgcgg tcgcatcggc ctatggcgga
     7981 tcgcgcgcca tcacctggcg ggaggtcctc gccggcgaaa aggcgttcaa tacgaccggc
     8041 aactggcttc ccgacgaaac gctcgacgcg atcaaggaat acaaggtcgc gatcaagggc
     8101 ccgctcacca cgccggtggg cggcgggatc cgcagtatca acgtcgccct tcgacaggtg
     8161 ctcaacctct atgcgtgcat acgccccgtt cgctacctca agggcgttcc cagccccgtc
     8221 gtccgtcccg atgcggtgaa tatgatcatc ttccgcgaga acatggagga cctctacgcc
     8281 ggcatcgaat ggaaggaggg ctctgaagaa gcgaagcgca tcatcgactt catccgatcc
     8341 gagcttaaga aggacatccg gccggactcc ggcattggca tcaagccgat cagcgagacc
     8401 aatacgaaaa acctcgtccg aagcgcgatc acctacgcca tcaagaacaa tcgctccagc
     8461 atcaccctgg tccacaaggg gaacatcatg aagttcaccg aaggggcgtt tcgcgaatgg
     8521 ggctacgaac tggccgcgaa ggagtttggc gaccacaccg tcaccgagga cgaactctgg
     8581 tcgaaattca acggaaaatg cccgcaggga aagatcgtca tcaaggaccg catcgccgat
     8641 gcgatgttcc agcaggtgat cctgcgtccc gacgaatatg acgtgatcgc aacaccgaac
     8701 ctgaacggcg actacctctc cgacgccatc gccgcgcaga ttggcggtct gggcatggcc
     8761 cccggcgcga acatcggcga cacgtccgcc gtattcgagg cgacccacgg cacggctccc
     8821 aagtatgcaa accaggacaa ggtgaacccg ggatccctga tcctttcggg cgagatgatg
     8881 ctgcgcttca tgggatggac cgaggcggcc gaccggatca tcacggccct cgaaaaaacg
     8941 atccagcaga agaaggtcac ctacgatctc gaacgccaga tgtcgggagc gaccctcctc
     9001 acctgctccg ccttcgggcg cgccgttgcg gagaacatgt aggcggtccg attattcgaa
     9061 ttccgcacgc acggcgtcgt cggtgagcgc gacgatgccg acgatctcga ggcaggcgac
     9121 gaaaaacctc gcgacgatta accccgcgtc gagcgcgtga tattcaaacg cgccggtcgc
     9181 gaggttgaag cacagcggca acgagtaggc tgcgtgaagc tccatcagtc caagctgcac
     9241 gtagcggctc atcggatgac ggaagaacaa tagccccatc tgtaccgcga caatcagcga
     9301 gagcgtcatc gcgacagcca gcgcgacccg gtttccgtag aggatgttca gggaaaccat
     9361 cagatagatg atctcaagaa aatagaaagc gttcagatag ggaagctggg cgactatcga
     9421 gccgcgaaat cgtccataga gtgcgatcca gtgagccatc gtatcaacgg tgaaagttca
     9481 ccagtacgtg gatatccgac tccggcatag ccggaacgct tgtctgcggt tcacgttgcg
     9541 ctggttcgcc caccaggaac cgcacggtct tgaagccgtc catgtcgctg ctcaccatgt
     9601 acggacgccg gccgcccagg tggtaaaagg tgttgagcat tcgattgaat tcgctggcgc
     9661 tcacccgccc ctcgacatag gaatcggtga ccgcgattat cttcgcccgg tgacgttcga
     9721 ggagcgacac cgtcgcgtct ttttccacat cgctcctgaa cacgttcatt gcgcgcgata
     9781 tccggttgtg cgcgtacgac acaggaaccg taacggcgcg cgggtcttcg ttccgggcga
     9841 cagtcgtcgg tcctgtcgtg ttgaaaaatc cgcccatggt cgcgaagatt gtcccggcta
     9901 tcacgaccga tgccgcgacg gccgccgtcg cagccgaacg gacagcccgc gatttcgaca
     9961 cccgatgcac gacatgcccg ccatccgata tccgacgcat cgtgttgcgc acgaaccgat
    10021 cggagtcgac aagcccgagc cccttgagcg acgagagcat ctgcagcatc ttccgtatgc
    10081 gtgcaagctc atccctgcag gaagcgcacc ccgcaaggtg ccgctcgatc gacgtcttgt
    10141 ccgcgacgga aagcggcgta ttgtcggcgt aggccgatag tctgtcgaaa gaaacatggt
    10201 ccatcatgac aggagccccc gttccgaaag ccgtttcttc agcatctcgc gcccccggtg
    10261 tatgcgcacc ttgacgttgg cgaggttaat cgaaagcatg gaactgatat cgttgtacga
    10321 gtacccttcg atgtcgcgca ggacgaggat ctcccggtaa tcgtcgggaa gcttgaacag
    10381 ctcctccttg acgatccgga gcgagtctcg gtcgatcacc cccgcctcga tatccacgcg
    10441 ctcgtcgtgg atctgcatct cgaattcggt gtcgtcgggg cggttgatgg agacgctcgc
    10501 ctgtctccgg ctcttcttct ggtggttttt gcagtagt
//
LOCUS       DAORHF010000121        10515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5595_length_10515_cov_6.522849, whole genome shotgun sequence.
ACCESSION   DAORHF010000121 DAORHF010000000
VERSION     DAORHF010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5595_length_10515_cov_6.522849"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PKJ16_10920"
     CDS             complement(<1..1879)
                     /locus_tag="PKJ16_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV47543.1"
                     /translation="MYTTRTEITRSPIRDYLALIGSMSKFVIGTAMYTPFTLLKRSSR
                     TGTNGSPANTRGHLGRQLTLRGALGFIANRFNASPRLRDQLMGTQGWINAIIGLRSDD
                     GLVEQAIIIRDGRITIADRIPENANVIVIFREEEDVLKQLQSSPEEGYKMILRGRIRV
                     EGNMMYMGFWDYLNNLIVGGSQKKAVLKQIEQHRKANQTLACGCSANGRQERRQRTSN
                     LLRGEKVDPGVKHLADPYLSTYTLGSFPRLERMRAERINATPEITAEYGKLVTDFFVE
                     NGYETKRDGSLWDPNIRTATCLKYIMENRKPLIRTGDLLAGTYTPNPVFGTVGHPNSL
                     GTFLWGELNTCRHRELEPFTITPETAEILHKHVFPFWVDRNITRWWNVEFDTPLTARI
                     HDRFFAVFFWKTTSNMENSPGFERVLKKGLAGLRAEIDRELREDANADEEKRNTLAAM
                     KIAMDGVSAYARNLAQRARDEAAAETDAARAQELEAMAGILDRVPDQPARTLHEAIQA
                     LWIMHISVGMENIDDGPSLGRLDQILQPYFEADIAQCSGDSAKETYIRNAIELLGSLF
                     LRINSHWPQTPDIGTLLNSGSPLNTTIVVGGVTEDGGDAVNDLSYLILKVTEMLVLND
                     PN"
     gene            complement(1968..2612)
                     /locus_tag="PKJ16_10925"
     CDS             complement(1968..2612)
                     /locus_tag="PKJ16_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47544.1"
                     /translation="MPKITRSNEEIDKVREQILDGAMEILATEGYESLSMNKVGQRMD
                     MTAANLYNYYENKNELFIAIHKKTFGMLHDRMREYVDAVDDPREKIAQLVRAFVEFGT
                     TNVHVYDIMFNRPIPQYTDYIGTPQEAMSFDEYQSSLKAFYFATETAAHYIALRPELK
                     GADPKYMTIRLLSALHGIVTLYNSRILFEIDPNPDEVLKRIQQDLLRQIDEGWL"
     gene            complement(2794..4473)
                     /locus_tag="PKJ16_10930"
     CDS             complement(2794..4473)
                     /locus_tag="PKJ16_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV47545.1"
                     /translation="MKIEKERAPRDAAPSASHKKGERSAEGSVPFSFRLFSAKDAPLW
                     TGERKHIVVIGSGIGGLAAGSLFAKIGHRVTVLEMNPELIGGHGRWLTFRGMRYSMGP
                     QYVWEFGPGEQGDRFLEFLGLRDDNPFVLMERDGFERIFIGNRDDEANSLFVNFKVPL
                     GLEHFRRDLIAMFPEEADRLDALFRDMIGMFSAYRRFFDEHSSPEGRIFLATKFLLTG
                     EAPLSHKLKLGQSLFLSVREWFDQYGISSLPRRILYGHGGIFAESESEMSAIAYIIGT
                     GNYHRGARYPLKGFHHFFASMASLIREHGGSVETGKRVVRLETSNALVTRAVCADGSA
                     YPCDFVFSDISSRLTYALLGMENAHFDYTPSHCIPTICIGLRKGLGSIALMKGRNYWW
                     QDGAEIDYDAPDITAPPRMLFVNSPTANMTADNETHDADALVVFCAGNYHQEREIHDR
                     GPEAVAAFKRKLASDIVDILDRNVFPGLRDHLLFAEVVSSIDIMEQTGGELGNAYGRR
                     MNVKEILKGQIEEENSPRNLYNVSATKNTPGIAGGISTAAILFEELTGRTI"
     gene            4677..5825
                     /locus_tag="PKJ16_10935"
     CDS             4677..5825
                     /locus_tag="PKJ16_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47546.1"
                     /translation="MHTTDAIYRRLQRHLDAQPIGFPRDPSGSDIRLLTHHFTVEEAR
                     VALGMSYRFETAAEIHARLRDGGDPEQTRAVLDGMSARTSIMRVERDGAVRYCLLPLV
                     IGMYEGKVFGLDREYARDFNDYANALQHGLTFASTEVMQMRVIPVETSLTPEQAILHY
                     DDVGRLVRESEGPIVVLDCICRKKRALLDERCVRTERLETCLAFGDIAALLSRNGQGR
                     TIGAPEARDILELNRRDGLVLQAYNMKRPEVICSCCGCCCGFLSVHRTLINPHHFWRT
                     NFRARIDENACTRCRRCVKICQVDALSYSRRKKRVMLDPSRCIGCGNCVTVCAPGAVR
                     LHALETPHVPPEDYEDLQETIMRHKPASRLGRIILRTLFGPPRRQTGP"
     gene            5940..6944
                     /locus_tag="PKJ16_10940"
     CDS             5940..6944
                     /locus_tag="PKJ16_10940"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008950525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV47547.1"
                     /translation="MKTTDTGRVETPHDDRSAMNEPESGLERAQGAPIAASRRHTLLP
                     YWLVGTLSSLMYIINVVVMFPFFFLLGILKFIIPIPAWQTLCRMITSKLGTLWVSVNT
                     MNLHITKRMRWDVTLPDDLRITESYLVISNHRSWSDILVLQKVFNRRIPFLTFFLKKE
                     LIWVPLLGLAWWALDFPFMKRYSEAYLKRHPQKRGKDIEITRRACEKFRNIPVSIMNF
                     VEGTRLTPEKHERQRSPFANLLKPKAGGIAFVLSTIGQELHKILNVTIAYPHGNESFW
                     GFVAGKVPAVKVYVEEFAITPEIIGDYANDEQFRERFQEWVNQLWIEKDRRLSSMLAE
                     "
     gene            7297..7746
                     /locus_tag="PKJ16_10945"
     CDS             7297..7746
                     /locus_tag="PKJ16_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV47548.1"
                     /translation="MLTRRGKIIAIIFGAAIVAVGSMIAVYLLIIAPSFVGRTGPDGA
                     MLKQYVDPAALKTLTERPREDIWIIDVRPASAYRAGHIPTARSFPSPEIESRMAELPR
                     EKYLIIYCETGGRVEAVISKLKRHGYMRYMNWGASYRWPYDWTTGAN"
     gene            7765..7950
                     /locus_tag="PKJ16_10950"
     CDS             7765..7950
                     /locus_tag="PKJ16_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47549.1"
                     /translation="MKTSQCENCTLRKKYDTHPQSLLGRFWKWHIRFCPGWKSYLKSV
                     PVDVRDQLFERYGRRAL"
     gene            8012..8455
                     /locus_tag="PKJ16_10955"
     CDS             8012..8455
                     /locus_tag="PKJ16_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV47550.1"
                     /translation="MTNDEALRLDNQLCFLLYAASRAVTRLYGPLLDGLGLTYPQYLT
                     MLVLWENDGVEVGTITRLLHMDTGTMSPLLKRLEAGGLIVRNRAKDDERKVIVSLTKK
                     GADLKRKAVSVPGALLCRSGLSFQDYAELKARLTDVLDTLERGAG"
     gene            8571..9428
                     /locus_tag="PKJ16_10960"
     CDS             8571..9428
                     /locus_tag="PKJ16_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HNV47551.1"
                     /translation="MTQETLARILDYQRNEITEHILYGALARRAKGKNAEILQMISDD
                     ELRHYHFFKKITGRDVRPARAKILFYRTVSRIFGFTFTIKMMDNGEVQAEHDYSEVED
                     RVPGIKKIIRDEVRHEAALMGQIEEDALKHMGSMVLAINNSIQEITGIAVGLTFALSN
                     ALLVGKTALISGLAATLAMVASEYLSQKAESADPNAPRRAALYTGIIYLFVVGAVVSP
                     FFIFAGHYAALGVAIGAVVLIVSVFTFFMSVVKGINYLKALAEVSLITAGVVALSLCL
                     GIGVKMLLG"
     gene            complement(9444..10502)
                     /locus_tag="PKJ16_10965"
     CDS             complement(9444..10502)
                     /locus_tag="PKJ16_10965"
                     /inference="COORDINATES: protein motif:HMM:NF016190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HNV47552.1"
                     /translation="MPSFIGLGVKDGNEHYRKNAITVNEIVVGSIAVREIRPEEREWR
                     IDVEIRANFTEEQAGARTRSAVIETWTLARARGTQSPEPERMQTICCPNCGAPAHFSV
                     SGACAHCGVAVKNGLMQWYIRVKRTDRTEKLDAGAAVTYAAERGTGLPTVMSPLLQQS
                     IETLCKTDSLEGWEPFWHYFSETIVRQSFFALAGAWTEKNWASARHLLTDRMYRVNNG
                     WIELYRRTSFTNRLDDLHLERIIPVKIATDRYYQAITVRIFASCFDYVVNADGKVVGG
                     SKRARRSFSEYWTFVRRVDSKKAAAEYRDITTCPACGASADRMGDTAQCGYCGSKISS
                     ASFSWVLGLITQDEEYEG"
BASE COUNT         1977 a         3333 c         3112 g         2093 t
ORIGIN      
        1 tgttgggatc gttcaggacc agcatctcgg tgaccttgag gatgagatac gacaggtcgt
       61 tcacggcatc gcctccgtcc tccgtcacgc cgcccacgac gatcgtggtg ttcagcggcg
      121 acccggagtt cagcagcgtg ccgatgtccg gcgtctgcgg ccaatggctg ttaatccgca
      181 ggaagagact gccgagaagt tcgatggcgt tacggatgta tgtctccttg gcggaatcgc
      241 ccgagcactg cgcgatatcg gcctcgaaat acggctgtaa aatttgatcc aggcgaccca
      301 gcgagggacc gtcgtcgatg ttctccattc ccacggagat gtgcatgatc cacaatgcct
      361 gaatggcctc atgaagggtc cgggcgggtt gatccggaac ccggtccagg atgccggcca
      421 tcgcctccag ctcctgtgcg cgcgccgcat ccgtttccgc agccgcctcg tcccgggcgc
      481 gttgcgcgag attgcgcgcg taggccgata cgccgtccat cgcgatcttc atcgccgcga
      541 gcgtgttccg cttctcctcg tcggcgttcg cgtcttcgcg aagctcccgg tctatctccg
      601 cgcgcaggcc cgcgaggccc ttcttcagca cccgctcgaa gcccggcgag ttctccatgt
      661 tggaggtcgt cttccagaag aagaccgcga aaaagcggtc gtggatccgc gcggtgagcg
      721 gcgtgtcgaa ctccacgttc caccagcggg tgatgtttcg gtccacccag aaggggaaca
      781 catgcttgtg aagtatctcg gcggtttcgg gggtgatggt gaacggctcc agctcgcgat
      841 gccggcaggt gttgagctcg ccccacagga aggtgcccag gctgttggga tgtcccaccg
      901 tgccgaagac gggatttggc gtataggtcc cggccagaag gtcgcccgtg cggatgaggg
      961 gcttccggtt ttccatgatg tacttcaggc acgtcgcggt ccggatattg ggatcccaaa
     1021 gtgagccgtc gcgtttcgtt tcgtatccgt tctccacgaa gaagtcggtc accagctttc
     1081 cgtattcggc cgtgatctcc ggcgtggcgt tgatgcgctc ggcgcgcatc cgttccagtc
     1141 gcggaaacga accgagcgtg taggtcgata gatacgggtc cgccagatgc ttcacgcccg
     1201 gatcgacctt ctctccccgt aagagattgc tggtccgctg acgccgttcc tgccgtccgt
     1261 tcgcgctgca gccgcaggcc agcgtttgat tggcctttcg gtgttgttcg atctgcttca
     1321 gcaccgcctt cttctggctg ccgccgacga tcaggttgtt cagatagtcc cagaagccca
     1381 tgtacatcat gttcccctcg acccttatcc ggccgcgcag gatcatcttg tatccctctt
     1441 ccgggctcga ttgcaactgc ttcaggacgt cctcctcctc ccggaagatg acgatcacgt
     1501 tggcgttctc gggaatccgg tcggcgatcg ttatccgacc gtcgcggatg atgatggcct
     1561 gctccaccag cccgtcgtcc gagcgcaggc cgatgatggc gttgatccat ccctgagtgc
     1621 ccatgagctg gtcccggagc cttgggctgg cgttgaagcg gttggcgata aaacccagcg
     1681 cgccgcgcag cgtgagctgc cggcccaggt gcccccgcgt gttggcaggg gacccgtttg
     1741 taccggtcct cgatgatcgc ttaagcagcg tgaacggcgt atacatggcc gttccgatga
     1801 cgaacttcga catgctgccg atgagcgcca ggtaatcgcg gatagggctt ctcgtgatct
     1861 cggtgcgggt ggtgtacatg atgtcgtctc cttaccgtgt gatgcgttta tgtcaatcat
     1921 tcccggggcc cgatagcgac ccaaagtctt tcttcgctac tgtggcgtta taaccagccc
     1981 tcgtcgatct gacgcaaaag gtcttgttgg atgcgtttca gaacctcgtc tggattgggg
     2041 tcgatctcga aaaggatccg gctattgtac agcgtaacga tgccgtgaag cgcgctcaag
     2101 agcctgatgg tcatgtattt ggggtcagca ccctttaact cgggccttaa ggcgatgtag
     2161 tgtgccgcgg tttcggtcgc gaagtagaac gccttaagcg agctctggta ctcgtcgaaa
     2221 gacatcgcct cttgaggcgt gccgatataa tcggtgtact ggggtatggg gcggttgaac
     2281 atgatgtcgt agacatgcac gttcgtcgta ccgaactcga cgaaggcccg gacgagctgg
     2341 gcgatctttt cccgaggatc gtccacggca tcgacgtact cccgcatcct gtcgtgaagc
     2401 atcccgaagg ttttcttgtg gatggcgatg aacagctcgt tcttgttctc gtagtagttg
     2461 tagaggttgg ccgcggtcat gtccatccgt tggcccacct tgttcatgga aagactctca
     2521 tatccctcgg tcgcaaggat ttccatggca ccgtcaagaa tctgctccct gactttatcg
     2581 atctcttcat tgcttcgagt tatttttggc atagtaattg atcattattc aatatctata
     2641 ttaatatcgt taatttaacg atattaaatt aaaaagtcaa ggggtgattt gaaaaaaacc
     2701 aaaaaaatat taatcgttat gcgaagtaat ggctctggaa tcggggagcg gacggactta
     2761 ccgatgcggt ttcttccctc gtgtggctac ctcctagatc gtcctccccg tcagctcctc
     2821 gaagaggatc gccgcagtgg agatcccgcc ggcaatgccc ggcgtattct tcgtggcgga
     2881 gacattgtag agattgcgcg gagagttctc ttcctcgatc tggcccttga gtatctcctt
     2941 cacgttcatc cggcgcccgt atgcgttccc gagctcgccg cccgtctgct ccatgatgtc
     3001 gatggagctc acgacctccg caaagaggag atggtcccgc aatcccggaa acacgttccg
     3061 gtcgaggata tcgacaatat ccgacgcgag tttcctcttg aaggcggcga ccgcctcggg
     3121 gccccggtcg tggatctcgc gctcctgatg gtagttcccc gcgcagaaca ccacgagcgc
     3181 gtcggcatcg tgcgtctcgt tgtccgcggt catgttcgcc gtgggcgagt tcacgaacag
     3241 catccgcggc ggcgcggtga tgtcgggcgc gtcgtagtcg atctcggcgc cgtcctgcca
     3301 ccagtagttt cgccccttca tgagcgctat tgagccaagg ccctttctga ggccgatgca
     3361 gatcgtcggg atgcagtgcg acggcgtgta atcgaaatgc gcattttcca tgccgaggag
     3421 cgcataggtc agccgcgacg agatgtcgct gaaaacgaaa tcgcacggat atgccgatcc
     3481 gtccgcgcac accgcgcgcg tcaccagggc attgctcgtc tccagccgca ccacgcgctt
     3541 tccggtctcg accgaaccgc cgtgctcgcg tatgagcgaa gccatcgacg caaagaaatg
     3601 gtggaatcct ttcaaaggat agcgcgcacc ccggtggtag ttcccggtcc cgatgatata
     3661 ggcgatggcc gacatctccg actccgactc ggcgaagatg ccgccgtgcc catacaggat
     3721 gcgccgcggc agcgagctga tgccgtattg gtcgaaccat tccctcaccg acagaaagag
     3781 cgactggccc agcttcagct tgtgcgacag gggcgcttcg ccggtcagca ggaacttggt
     3841 cgccaggaag atgcggccct ccgggctcga gtgctcgtcg aagaaacgcc gataggccga
     3901 gaacatcccg atcatatcgc gaaagagcgc gtccagccgg tcggcctcct ccgggaacat
     3961 cgcgatcagg tcgcgccgaa agtgctcgag ccccagcggc accttgaagt tcacgaacag
     4021 cgaattggcc tcatcgtcgc ggttcccgat gaagatccgc tcgaagccgt cgcgctccat
     4081 cagaacaaac gggttgtcgt ccctgaggcc caggaactcg aggaatcgat ccccctgctc
     4141 ccccggaccg aactcccaca cgtactgcgg ccccatcgaa tagcgcatcc cgcggaacgt
     4201 gagccagcgc ccgtgcccgc cgatgagctc cgggttcatc tccagcaccg tgacgcgatg
     4261 cccgatcttc gcgaagagac tcccggcggc gagtcccccg atgccgcttc cgatcaccac
     4321 gatatgtttc ctctcgcccg tccacagcgg cgcgtccttc gcgctaaaca gccggaagga
     4381 aaacggcacc gagccctcgg cgctccgttc cccttttttg tgtgatgctg atggcgccgc
     4441 gtcgcgcggc gcgcgctcct tttctatctt catgatatgc ttctcgcatc tcccgtgttt
     4501 tcgtcgcccg gcgttgcagt cggcgcacgt ttcaaaacgg ccaacgcact atcccccgca
     4561 gtgccgacat gcaagcaaaa aatcgccgat atttcgagcg caatgggcga aaaaaaattt
     4621 gccatgcgcg cccgtcgctg ctttcatatc atccgatcgc gcccctggcc aaacccatgc
     4681 acaccaccga cgccatatat cgccgattgc agcggcacct cgacgcgcag cccatcggct
     4741 tcccccgcga cccgagcggc tccgacatcc gcctgctcac gcaccacttc accgtcgagg
     4801 aagcgcgcgt cgccctgggg atgagctatc gcttcgagac ggccgcggag atccatgcgc
     4861 gcctccggga cggcggcgat ccggagcaga cgcgcgcggt cctcgacggc atgagcgcgc
     4921 ggacgtcaat catgcgcgtg gagcgcgacg gcgcggtgcg ctactgcctg ctcccgctgg
     4981 tgatcggcat gtacgagggc aaggtcttcg ggctggaccg cgagtacgcg cgcgacttca
     5041 acgactacgc gaacgcgctc cagcacggcc tcaccttcgc cagcaccgag gtgatgcaga
     5101 tgcgcgtgat tccggtggaa acgagcctca cgcccgaaca ggccatcctc cactacgacg
     5161 acgtggggcg cctcgtgcgc gaatcggagg gccccatcgt ggtcctggac tgcatctgcc
     5221 ggaaaaagcg cgccctgctg gatgagcgct gcgtgcggac ggagcgcctc gagacctgcc
     5281 tggccttcgg cgacatcgcc gcgctgcttt cgagaaacgg gcagggcagg accatcggcg
     5341 cccccgaggc gcgcgatatc ctggagctca atcgacgcga cggcctggtc ctgcaggcct
     5401 ataacatgaa gcggcccgag gtcatctgct cctgctgcgg ctgctgctgc ggcttcctca
     5461 gcgtccaccg gacgctcatc aacccccacc acttctggcg cacaaatttc cgcgcgcgca
     5521 tcgatgagaa cgcctgcacc cgctgccggc gctgcgtgaa gatctgccag gtggacgcgc
     5581 tctcgtattc ccgccggaag aagcgcgtca tgctcgatcc gtcccgctgc atcggctgcg
     5641 ggaactgcgt caccgtgtgc gcgcccgggg ccgtccggct ccatgcgctg gagacgccgc
     5701 atgtcccgcc ggaggattac gaggaccttc aggagacgat catgcgccac aaacccgcat
     5761 cgcggctcgg ccggatcata ctgcgcacgc tctttgggcc accgcgccgg caaaccggtc
     5821 cctgaaacgg ccccctgaac ttttttcttg aacggtcgtt tttttgcacc cgtcccggcc
     5881 ccattcccgt cgttctcaag aagcaagaat tttgatagac cgtggagcgg gatgcaacta
     5941 tgaagaccac agacaccgga cgcgtcgaaa caccacacga cgacaggagc gccatgaacg
     6001 aaccggaatc cggactcgaa cgagcgcagg gagcgcccat tgcggcgagc aggcgtcata
     6061 ccctgctgcc gtactggctc gtgggaacgc tgtcgagctt aatgtacatc atcaatgtcg
     6121 tcgtgatgtt tcccttcttt ttcctgctgg gcatccttaa attcatcatt ccgataccgg
     6181 cgtggcaaac cctgtgccgc atgatcacca gcaagttggg gacgctttgg gtttcggtga
     6241 acaccatgaa tctgcatatc accaagcgca tgcgctggga cgtcacgctt ccggacgacc
     6301 tgcgcatcac cgagtcgtac ctggtgatct cgaaccatcg ctcctggtcc gacatcctcg
     6361 tgctccagaa ggtcttcaac cgccgcatcc cgttcctgac cttttttctc aagaaggagc
     6421 tcatctgggt gcccctcctg ggcctcgcct ggtgggcgct cgatttcccc ttcatgaagc
     6481 gctattcgga ggcctatctg aagcgccacc cgcaaaagcg cggcaaggac atcgagatca
     6541 cgcggcgcgc ctgcgaaaag ttccggaaca tccccgtgtc catcatgaat ttcgtcgagg
     6601 gaacgcgcct caccccggag aagcacgaac ggcagcgctc gcccttcgcc aatctattga
     6661 agcccaaggc gggcggcatc gccttcgttc tgtccaccat cggccaggag ctgcataaaa
     6721 tcctcaacgt gaccatcgcc tatccgcatg ggaacgagag cttctgggga ttcgtggcgg
     6781 ggaaggtccc cgccgtgaag gtgtatgtgg aggagttcgc catcaccccg gagatcatcg
     6841 gcgattacgc caacgacgag cagttccggg aacgcttcca ggaatgggtg aaccagctct
     6901 ggatcgagaa ggaccgccgg ctgtcgtcca tgctcgccga gtaagcggac gcgcggcccg
     6961 catcaccgaa accagccgtc gtctctcgtc gcgcgcggat ccgcttttcg tccggagcca
     7021 acccgttcgc acctccgtgg ggctgtcgaa atcgggcgca taaattccct tagcgccatt
     7081 ttcgcacatg ggccgtatgg gcgtccccgc gctctccccg aacggtgttt cgataggacc
     7141 gattgaccag aaaagatact cgttctgttt catgacgcat atcatccgac gaccaaatat
     7201 attgtgtaaa atataacttg acacaatata tattcgccca tcatggttcc agtgtgcaga
     7261 gcgatgcaca acagtcaaac gcaaaggaga acagatatgc taactcgtcg tgggaaaata
     7321 attgccatta tttttggggc ggctatcgtg gccgtgggct ccatgatcgc ggtctacctg
     7381 ctcatcatcg ccccgtcgtt cgtgggacgc acgggaccgg acggcgcgat gctgaaacaa
     7441 tacgtcgatc cggccgcgct caagacgctc accgagcggc cccgcgagga catctggatc
     7501 atcgacgttc gtccggcgag tgcataccgc gcgggccaca tccccacggc gcgttcgttt
     7561 ccctcgcctg agatcgaatc gcgcatggcc gagctgcccc gcgagaagta cctgatcatt
     7621 tactgcgaaa ccggtgggcg ggtggaggcg gtcatcagta aactgaagcg gcacggctac
     7681 atgcgctata tgaactgggg cgcctcgtat cgatggccgt acgactggac cacgggcgcc
     7741 aactaattca ccaggaggac aatcatgaaa acctcacaat gtgagaactg cacccttcgc
     7801 aaaaagtacg acacccatcc gcaatcgctg ttgggccgat tctggaagtg gcacattcgg
     7861 ttttgcccgg gatggaaatc gtatctgaag agcgttcccg tggacgtgcg cgaccagctc
     7921 ttcgagcgct acggccggcg ggcgctgtag ccgcgccgac gcggatcgcg cagttcgtga
     7981 cgaacgcgag cgcgccttac gggacatcgc aatgacgaac gacgaagcct tgcggctcga
     8041 caaccagctg tgcttcctcc tgtacgcggc gtcgcgcgcc gttacgcgcc tgtacgggcc
     8101 gctcctcgac gggctggggc tcacctaccc gcagtacctc accatgctgg tgctctggga
     8161 aaacgatggc gtcgaggtgg ggaccatcac gcggctgctg cacatggaca ccggcacgat
     8221 gagcccgctc ctgaagcgcc tcgaggcggg cggcctcatc gtccggaacc gcgcgaagga
     8281 cgacgagcga aaggtgatcg tctcgctcac gaaaaagggc gccgatttga agcggaaggc
     8341 cgtatcggtc ccgggcgctc tcctgtgccg gtcggggctg tcgtttcagg attacgccga
     8401 gctgaaggcc aggctcacag atgtgctgga cacgctcgag cgcggcgcgg gctaacggac
     8461 aaaacacagg cgcgtacggc cggggttgat gccgcatgcg cgccgccgcc agcgtccgtc
     8521 gtgcaaatag cgcatcgtca catccccgta caaccttcac ggagaacgtc atgacccaag
     8581 agacccttgc gagaatcctg gactaccaga gaaacgagat taccgaacac atcctctacg
     8641 gggcgctcgc ccggcgcgca aaagggaaga acgccgaaat cctacaaatg atatcggacg
     8701 acgaactgcg gcattatcac ttcttcaaga agatcacggg acgtgacgtg cggccggcgc
     8761 gcgcgaagat cctgttctat cggaccgtgt cgcgcatctt cggtttcacc ttcaccatca
     8821 agatgatgga caacggcgag gtgcaggccg agcacgatta cagcgaggtc gaggaccgcg
     8881 tgcccggcat caagaagatc atccgcgacg aggtgcgcca cgaggcggcg ctcatggggc
     8941 agatcgagga ggacgcgctc aagcacatgg gctcgatggt gctggccatc aacaattcga
     9001 tccaggaaat caccgggatc gcggtggggc tcaccttcgc gttgtccaat gcgctcctcg
     9061 tcggcaagac ggcgctcatc agcggcctgg ccgcgacgct cgcgatggtc gcctcggagt
     9121 atctgtcgca aaaagccgag agcgccgacc ccaacgcccc gcgccgggcg gcgctctaca
     9181 cggggatcat ctatctcttc gtggtgggcg ccgtcgtgag tccctttttc attttcgcgg
     9241 gccattacgc ggcgctgggc gtggccatcg gcgccgtcgt gctcatcgtg tcggtcttca
     9301 cgttcttcat gtcggtcgtg aaggggatca actacctcaa ggcgctcgcc gaggtctcgc
     9361 tcatcacggc gggcgtggtc gcgctctcgc tgtgcctcgg gatcggcgtg aagatgctgt
     9421 tgggctgacg cctgcggccc gccctatccc tcgtattcct cgtcctgggt gatgaggccg
     9481 agcacccacg aaaacgacgc cgagcttatt ttcgatccgc agtagccgca ctgcgcggta
     9541 tcgcccattc ggtccgccga cgcgccgcag gccgggcagg tcgtgatatc gcgatactcc
     9601 gcggccgcct tcttcgaatc gacccgccgc acgaaggtcc agtactcgga gaaggaacgc
     9661 cgcgcgcgct tcgacccgcc cacgaccttc ccgtccgcgt tcacgacata atcgaagcag
     9721 gatgcgaaga tccgcaccgt gatcgcctga tagtatcgat cggtggcgat cttcaccgga
     9781 atgatgcgct ccaggtgcag gtcgtcgagc cggttcgtga aggaggtgcg gcggtacaat
     9841 tcgatccagc cgttgttcac ccggtacatc cggtcggtga gcaggtgccg cgcgctcgcc
     9901 cagttcttct cggtccaggc cccggcaagc gcgaaaaacg actgccgcac gatggtttcg
     9961 gagaaatagt gccagaacgg ctcccagccc tccagggaat cggttttgca cagcgtttca
    10021 atcgactgct gtaataacgg gctcatgaca gtcggcaggc cggtcccgcg ctcggccgca
    10081 tacgtgaccg ccgcgcccgc atcgagcttc tcggtgcggt ccgtgcgctt gacgcggatg
    10141 taccattgca tgagcccgtt tttcaccgcg acgccgcaat gggcgcatgc gccggaaacc
    10201 gaaaaatgcg ccggggcccc gcagttcgga cagcagatcg tctgcatgcg ctcgggctcg
    10261 ggcgactgcg tcccgcgggc gcgcgccagg gtccaggttt caatcaccgc agagcgcgtc
    10321 cgcgcgccgg cctgctcctc ggtgaagttc gcccggatct ccacgtcgat gcgccactcg
    10381 cgctcctcgg gccgtatctc gcgcacggca atgcttccca ccacgatctc gttcacggtg
    10441 atggcgtttt tccggtagtg ttcgttgccg tccttcaccc cgagaccgat gaaggacggc
    10501 aacgaacact accgg
//
LOCUS       DAORHF010000122        10485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5618_length_10485_cov_4.942761, whole genome shotgun sequence.
ACCESSION   DAORHF010000122 DAORHF010000000
VERSION     DAORHF010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10485
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5618_length_10485_cov_4.942761"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PKJ16_10970"
     CDS             <1..235
                     /locus_tag="PKJ16_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47553.1"
                     /translation="VVQMDKVHGNVLCVRLNTRFVMSDGAHMRGISASTVFDTFRAGD
                     IISSTILKILSPPVFKLTDLTKKIIVHREHHIS"
     gene            260..436
                     /locus_tag="PKJ16_10975"
     CDS             260..436
                     /locus_tag="PKJ16_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HNV47554.1"
                     /translation="MIDTKLLEILACPACRGDVEYDTKNEKIICTECGRKYPVRDGIP
                     VMLIEEAEQPAKSS"
     gene            501..1652
                     /locus_tag="PKJ16_10980"
     CDS             501..1652
                     /locus_tag="PKJ16_10980"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HNV47555.1"
                     /translation="MFDSKSPVVKVVGYIILGFLTLIIIISFGMPDFMSRLGVDQSTI
                     AVVNGEKIHASEFLRFRDMYARNLKGMESGQQTQLILNNLIRYKLQQQLAAKIGIKVS
                     DERVKQQIRSLPYFQDPPGTFSGERFKTIMSANRLTISDFYETVRSDLVNGEIMHLFK
                     NGAGAVPDEVLKQYIMDNAQVQIQFCVVTTAELKERHRAAIAVTDVEIDDEMKKNRSE
                     IKDPKTDRDRIRKKLEDKKFEAFRTKLLASIDELAIAGKSFGEAAALMRGRVGQSERF
                     KIGEPVREPGPAGKALTSLSGSKVFVEDCLALETGKASKAISAGDGIYVFTPVQKSIP
                     DSIPPTVKADGLTARLADERATLSYMAVMTRFGEKSKIIRNLKDKDVQE"
     gene            1669..2133
                     /locus_tag="PKJ16_10985"
     CDS             1669..2133
                     /locus_tag="PKJ16_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNV47556.1"
                     /translation="MNINAEYVNPFLEAASAVFKSLLNVDLRRGKLTIKEKPEPSHDI
                     AIIIGITGAANGEVVFSMEHAMVQKIADILTPGLKPEDLKSEYRDIVGELANMITGNA
                     MNLFAYSGKRVEMTTPTVVEGKEFTITLVKQTTLAINLYSPFGQLEMNVALK"
     gene            complement(2141..3010)
                     /locus_tag="PKJ16_10990"
     CDS             complement(2141..3010)
                     /locus_tag="PKJ16_10990"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47557.1"
                     /translation="MWTTLICGIAVLAVAIGSASAKEKPEDIIKYILTSETDSAAEKP
                     KFRTPTRSATAKPSSTKPSEQKQKKQEKLAPGSPEEVIYKTAIQLGNASHNEAALSKF
                     AELKSKYPQSVYRDSASVWSGRIHLRLRNVAVALKEFESIAESSGEYPAALFYLGRSH
                     AIQGARTKAIEHYFKLATQFPDVDLADNALIELSQLYLDDGRGAQALEMAAKVIRQYP
                     DRDTIDEAYYLVGKIFERDPVLKDIETARKMYQIFLRKAQRKDPHFAQSPLRRQVARD
                     LKNIERIYFNLEN"
     gene            complement(3019..3735)
                     /locus_tag="PKJ16_10995"
     CDS             complement(3019..3735)
                     /locus_tag="PKJ16_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47558.1"
                     /translation="MNPLVKIASGLISTINRIIDSVDTKTADGIRQGFFFVIFLIAIG
                     AVIMGYRLGSGSAKRTGIPLAESTNQVFEIDIKRQRDHAQFNTLLDTESVRETERVDP
                     EKNKFLSREGVDMESSDRLAEPDSMKKKSPPAFDVDTRSRIAEIDRTGDTPRQSGVRG
                     LPRRESVIDRPKKPELVGDEKEKAQETDDRARDIRDSVPAKTQQLDTPRQESKKRPLV
                     KMRPERSGRSSDRQPGVIDR"
     gene            complement(3808..4659)
                     /locus_tag="PKJ16_11000"
     CDS             complement(3808..4659)
                     /locus_tag="PKJ16_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47559.1"
                     /translation="MYMKLMTIMALIAAVSAAPLYARDYVTKTNAGSVNWTRGTIVTA
                     AASRLSMSDDGQIMDAHGIQSIPLNRARMESYAHARERSIENAVNILRGVRIDPETTF
                     GDLLKNDRITQQRLSDVLERAARTKKQPSGFFATSCETTIRLRDIVPALNYEFPAVAF
                     PERDDAQLATRYTGVIIDGRGIDIQPMLFPSIYTEDGLELYGRHHIDIRYASRRGLAA
                     YCYNENDAVKNRRAGDRPFYTTALTSIRNCPVIAESDARKILGSPATRENLKKCRVII
                     ILSAKKR"
     gene            complement(4680..6383)
                     /locus_tag="PKJ16_11005"
     CDS             complement(4680..6383)
                     /locus_tag="PKJ16_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV47560.1"
                     /translation="MGTYAVQDTPEGTKTLIFTRDREIRLHSAYGPVREASRSVQSFS
                     VGRANLIVVLGCGLGYHVTLLRERFPECDLLVVERDREVVDLCDTHNPSSLAGAIVVT
                     TPRDLSGYIESLDMARFHGARFYTHRPSYLIDAEFYDAAMRDINQYLSSKLSDLLTRF
                     EFEARWVENILCNLRHLERAHAVHDLFGAFSGIPGVIVSAGPSLRKNVGHLAALRSRA
                     LIIAVDTAYPVLRRLGIEPHIVMTLDSQKHSLKHFLGHEDSAAALVADIVSYPRILDR
                     YRGPLIMSSTAKYYQDAGGGMNRETTPFMDWVESHIEPFGDIQSGGSIATNAFDLLLN
                     AGCAPIILVGQDLAYTGREIHCSGTHHNDGWLPITNRMRNLETINQSVVRRRTIKYVD
                     ACGAPTTVLTDFVFDLYRNWFSDSAAKVPCTVINATEGGARIENTVETPLRQLVDDIP
                     IPATAPADILTRILSRPSGRDLTRLRRALAAARETVQAIHDLSNGHVDSTDPLIADRI
                     ETLADDPMVRQMFAPFLKKTRSYLMRHGELEASKSRELILADIATAAKKLLAFISRSK
                     L"
     gene            complement(6402..7799)
                     /locus_tag="PKJ16_11010"
     CDS             complement(6402..7799)
                     /locus_tag="PKJ16_11010"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV47561.1"
                     /translation="MQSINSLLAKLLANFDQIFLLDTVTTILREIIINATKANAKRVY
                     FKKINLDITKPNEYTEGIQNFKNNVIGNLEELAEDMKKSDLKVSMNINKENGGFKITV
                     TNDVPILPEELERINLRKAKAIEYNDFTDAYDEIYDSTEGAGLGIVLIMLLLKNSGIN
                     TSSYVLNSDGKKTWSWIVIPGQTRPNEITTEIKKQIINEVNGLPTFPKNIIELQAMCK
                     DSTVDIDDIAKKIMLDPALTTDVLKLSNSAGFLTQRRIDNVNEALIKIGLKNLNSILV
                     AASARRILDKRFSKFEQIWTHCNKTAFYARNIAQRYKMGAILEHSFLAGLLHDLGKIV
                     LLSTDTKITNWISDIVKDRKMRVSTVMEEVSIGISHSSIGELIARKWQFPEYLIEAIK
                     YHHSPLSASEEYRDLVYTTYLANMMCGIESRKYGYYYLDEDVLERFKINGPEAFGKFH
                     EEFKTMLANNTAKEQ"
     gene            complement(7995..9866)
                     /locus_tag="PKJ16_11015"
     CDS             complement(7995..9866)
                     /locus_tag="PKJ16_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HNV47562.1"
                     /translation="MQTKDFSIVRHVLIPLALGATVLARVIHLPAGDFPHDPARIYCI
                     ADIQERRYDTHLTLIPDGSLQIAASRQALGIIRRPGELASGDRVRISAFPRAIQRQNG
                     PTDGWAVDRLRKGVSAVFYLDEKTISVVEKTSPSLRRDVRKRIEEALDRLYRPETARM
                     IEALYFGNQHVITKRVQDEFRRAGVLHILAASGMNVGLIALIPLAFFGALRTDRKAAM
                     AATLVAVSGYLALTDVPVSLFRAYVMFAIYTIQKLFDLDRNIINTLLWSAVVILIVAP
                     WELYGLGFQLSFGATLGILLFARPLSTAFRALPGPIASSLSVTCAAQIPVYPILLLQV
                     HELNLVGIITNLIAVPGMSVMLIGSIAVTALSALSPPLAHLLAPAIERLFDCNHQFIR
                     TCAHIPGHFIINEPAPLLLIPYVFLVLTLAPIRRFWIVAPSSLALAYVGAWLYCASVQ
                     PPTGPSLSVLKNTHSQAVVFTGPRVAVVAGHLGSFEDARAVDAALTASQPSETHIAIT
                     KADYASVRYFVHIIKRQKIASVHVSDDFRINVAFRRLCHALDADRVPLKIIPSRSGSG
                     ARPETARDRLFASSSSVPPIDLVLALDSHLARIGALAFATMISAGPDAPRCSILNQQ"
     gene            complement(10138..>10485)
                     /locus_tag="PKJ16_11020"
     CDS             complement(10138..>10485)
                     /locus_tag="PKJ16_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47563.1"
                     /translation="GEFSLQYISSANAGTPDVSASYTMATALGKFAWFFFDVVGVAGG
                     VGMYLEIPPFKKDYTGGAGFAGSIGLVFNLGTDVKIFVDGIARTGRFGFGEESTITSF
                     GGRIGVMYNVGRL"
BASE COUNT         2198 a         2734 c         3247 g         2306 t
ORIGIN      
        1 ggttgtgcaa atggataaag tccacggcaa tgtcctgtgc gtacggttga atacgcgatt
       61 cgtgatgtcg gatggcgcgc atatgcgggg gatttccgca tcgacggtgt ttgatacctt
      121 ccgtgcaggg gacataattt caagcacaat tttaaaaatt ttatcgccgc ccgtttttaa
      181 attgactgat ttgacaaaga aaataatagt acatcgagaa caccatattt cgtaagaata
      241 ataattcgca gcgggtgcca tgatagacac gaaacttctc gaaatactcg cctgcccggc
      301 ctgtcggggc gacgtggagt acgatacgaa aaatgaaaaa atcatttgta ccgagtgcgg
      361 tagaaaatac ccggtgcggg acggcatacc cgtcatgctc atagaggaag cggagcagcc
      421 ggcgaaatct tcatgacgtc ccggagcgac cgtctcaggc tcgcggcata tcatacccaa
      481 aaaggaacgg tgcggcacac atgttcgatt cgaaatcccc ggtcgtaaaa gtagtcggat
      541 acatcatact tggcttcctc acgctgatca tcatcatatc attcggcatg cctgacttca
      601 tgtcgcgcct cggcgtcgat cagagtacaa tcgccgtggt caacggcgaa aagatacatg
      661 cgagcgagtt tctgcggttt cgcgacatgt acgccaggaa cctcaaggga atggaatcgg
      721 ggcagcaaac gcagcttatc ctgaacaacc tgatcaggta caagcttcaa cagcagctcg
      781 ccgcgaaaat cggaatcaag gtttccgacg agcgcgtgaa gcagcagatc cgctctctgc
      841 cgtactttca ggacccgccg ggaaccttca gcggcgagcg gtttaagacc atcatgagcg
      901 ccaatcggct gaccatttcc gatttctacg aaaccgtccg cagcgacctg gtcaatggcg
      961 agatcatgca tctgtttaaa aacggggccg gggcagtgcc cgacgaggtg ctcaagcaat
     1021 acatcatgga caatgcgcag gtgcagatcc agttctgcgt tgtcaccacg gccgagctca
     1081 aggagcgcca ccgggccgcg atcgccgtga ccgacgtcga gattgacgat gagatgaaga
     1141 agaatcgaag cgagatcaag gatcccaaaa ccgatcgcga tcggatcagg aaaaaattgg
     1201 aagacaagaa attcgaggca ttccgcacga aactgcttgc atcgatcgac gagctggcca
     1261 tcgccggaaa gtccttcggt gaggccgccg cgctcatgcg gggacgggtg gggcaatcgg
     1321 agcgctttaa gatcggagag ccggtcaggg agcccggtcc ggcgggcaag gcgctcacct
     1381 cgctcagtgg atcgaaggtg ttcgtcgagg attgccttgc gctcgaaacg gggaaggcgt
     1441 ccaaggcgat cagcgccggt gacggcatct acgtgttcac cccagtgcaa aagtcgattc
     1501 ccgattcgat tccgccgacc gtgaaagcgg acggcctgac ggcacgtttg gccgacgaac
     1561 gcgccacgct gtcatacatg gcggtcatga cgcgttttgg cgaaaagtcc aagataatac
     1621 gaaatctcaa ggataaggac gtccaagaat aaccaacgcg aggatggcat gaatatcaat
     1681 gcagaatatg tgaacccctt tctcgaggcc gcgagcgccg tatttaaatc gctcttgaac
     1741 gtcgatcttc gccggggaaa gctcaccatc aaggagaagc cggagccgtc ccacgatatc
     1801 gccatcatta tcgggatcac tggcgcggcg aacggggagg tcgtgttcag catggagcac
     1861 gccatggtgc agaaaatcgc ggacattctc actcccggtt tgaaaccgga ggatctgaaa
     1921 agcgaatatc gggacatcgt gggagaactt gcgaatatga ttaccgggaa tgcgatgaat
     1981 ctgttcgcct attccggaaa gcgggtcgag atgacgaccc cgacggtcgt ggaaggtaag
     2041 gagttcacca taacgctcgt caagcagacc acgctcgcga tcaatctcta cagccccttc
     2101 ggacagctcg agatgaacgt ggccctcaag tgagcggacg tcagttttcc agattgaagt
     2161 aaatccgctc gatatttttc aggtcgcgcg ccacctgccg gcgaagcggc gattgcgcaa
     2221 agtgcggatc cttgcgttgc gcctttcgta aaaaaatctg atacatcttc cgcgccgttt
     2281 cgatgtcctt gaggacgggg tcccgctcga atattttgcc gaccagataa tacgcctcgt
     2341 cgatggtgtc gcgatcggga tattgccgta tgaccttcgc ggccatctca agcgcctgcg
     2401 cgccgcgtcc gtcgtcgagg tataattgcg agagttcgat gagtgcgttg tcggcgagat
     2461 cgacatcggg gaactgcgtc gcgagcttga agtagtgctc gatggccttg gtccgggcgc
     2521 cctgaatcgc gtgcgaacgg ccgagataga acaatgccgc gggatactcg ccggaagact
     2581 cggcgatcga ttcaaattcc ttgagcgcca cggctacatt cctgagccgg agatgaatcc
     2641 ggcccgacca gacggacgcg ctgtcccgat atacgctttg aggatatttc gatttaagct
     2701 cggcgaattt cgagagggcc gcctcgttgt gagaggcatt tccaagttgg atggcggttt
     2761 tatagatcac ctcttcgggt gagccggggg cgagcttttc ctgttttttt tgtttctgct
     2821 cggacggttt tgtcgatgag ggtttcgcgg tggcgcttcg ggtcggtgtg cgaaatttcg
     2881 gtttttccgc ggcggaatcg gtctccgagg tgagtatgta tttgatgatg tcttccggct
     2941 tttcttttgc gctcgcggaa ccgatcgcga cggcgagcac ggcgatcccg cagatcagcg
     3001 ttgtccacat gtatcgcgtc atcggtcgat cacacccggt tggcgatccg aggatcggcc
     3061 cgagcgttcg ggacgcattt tcacgagggg ccgttttttc gattcctgac ggggcgtatc
     3121 cagctgttgc gttttagcgg ggacgctatc gcgaatatct cgtgctcgat cgtcggtttc
     3181 ctgcgccttc tccttctcat ctccgaccag ttcaggtttt ttcggacggt cgatgacgga
     3241 ctcacggcgt ggaagtcccc gcacgcccga ctgccggggc gtatcgccgg tgcggtcgat
     3301 ctcggcgatt cgactcctgg tgtcgacgtc gaaggccggg ggtgatttct ttttcatgga
     3361 gtcgggctcg gccaaacggt ccgatgactc catgtcgaca ccctcacggg acagaaactt
     3421 gtttttttcg gggtcgacgc gttcggtttc acgaacggat tccgtatcga ggagggtgtt
     3481 gaattgcgcg tgatcccgct ggcgcttgat gtcgatctcg aagacctgat tggtgctctc
     3541 ggcaaggggg atccccgtgc gcttcgcaga tccgcttccc aggcgatatc ccatgatgac
     3601 ggcgccgatg gcgatcagga atatcacaaa gaaaaaaccc tggcggattc cgtcggctgt
     3661 tttggtgtca accgaatcga taatgcggtt gatggtggag atgagacctg aagcaatttt
     3721 aaccagtgga ttcatcggcg cgaatgcaat gacggctccg tattatgtca caaaggtatc
     3781 ggttcgacgg cgcgaacgtc aatcatttta tctttttttt gcgctcagaa tgattattac
     3841 gcggcatttt ttcaggtttt cgcgtgttgc cgggctcccg agtatcttcc gggcgtcgct
     3901 ttccgcgatg accgggcaat tgcgtatgct cgtgagggcg gtcgtgtaga agggacggtc
     3961 tcccgcgcga cggttcttca ccgcatcgtt ctcgttataa cagtacgcag cgagacctcg
     4021 tcgcgacgca tagcgaatgt cgatatgatg ccgaccgtac aactcaagcc catcctcggt
     4081 gtagatcgac gggaacaaca tcggctggat gtcgataccg cgaccgtcga tgataacgcc
     4141 agtatagcgg gttgcgagct gggcgtcgtc ccgttcggga aaggcgaccg cgggaaattc
     4201 gtaattcaac gccggtacga tgtcacggag ccgtatcgta gtttcgcacg aggtggcgaa
     4261 aaagcccgat ggctgctttt tcgtccgggc ggcgcgttcc agaacatcgg acagccgctg
     4321 ctgggtgatt cggtcgttct tcaggagatc gccaaaggtc gtttcggggt cgatccggac
     4381 gccgcgcagg atgttcaccg cgttctcgat cgaccgctcc cgcgcgtgtg cgtaggattc
     4441 catgcgcgcg cggttcagcg gtatcgattg tatgccgtgc gcgtccatga tctgtccgtc
     4501 atcgctcatg gaaagacgcg aggctgcggc ggtgacgatg gtcccgcgcg tccagttgac
     4561 ggatccggcg tttgttttcg ttacatagtc cctggcgtag agcggcgccg cggagacggc
     4621 cgcgataagg gccatgatgg tcatcagttt catatacatc agtatcggcc agcggacggc
     4681 tataatttac tgcgggaaat gaaggcgagg agctttttgg ccgcagtggc gatatcagcc
     4741 agaatgagtt cgcgtgattt cgacgcctca agttcgccat ggcgcatcag atacgaccgg
     4801 gttttcttca ggaagggggc gaacatttgc cgtaccatgg ggtcgtcggc cagggtttcg
     4861 atgcgatcgg cgatgagcgg atccgttgaa tcgacatggc cgttcgagag atcgtgtatc
     4921 gcttgaaccg tttcacgcgc ggcagccagt gcccgtctca ggcgcgtgag gtcccggccc
     4981 gaggggcggg acaggattcg agtgaggatg tcggccgggg cggttgcggg gattggaata
     5041 tcatcgacga gctggcgcaa tggcgtttcg acggtgttct caatgcgcgc gccgccctca
     5101 gtggcattaa tgaccgtaca gggcaccttc gcggcggagt cggagaacca gtttcgatac
     5161 aggtcgaata cgaaatcggt gagcaccgtc gtgggtgcgc cgcaggcatc aacatacttg
     5221 atggtgcggc gacggacgac cgactggttg atcgtctcga ggtttcgcat ccggttcgtg
     5281 atcgggagcc agccgtcatt gtggtgcgtg ccggagcaat ggatctcgcg tccggtatag
     5341 gccaggtcct gtcccaccag tatgatgggc gcgcacccgg cattgagcag cagatcgaag
     5401 gcgtttgttg cgatggatcc gccggactgg atgtcgccga agggttcgat gtgcgactcc
     5461 acccagtcca tgaatggcgt cgtttcgcga ttcatcccgc caccggcgtc ctgataatat
     5521 ttcgccgttg agctcatgat gagcggtccc cggtagcggt cgaggatgcg cggatagctg
     5581 acgatatcgg cgaccagggc cgccgctgaa tcttcatggc cgagaaagtg cttgaggctg
     5641 tgtttctgcg agtccagcgt catgacgatg tgcggttcga taccgagccg tctgagaacg
     5701 gggtaggcgg tgtcgaccgc gatgatcagg gcccgcgagc ggagcgcggc caggtggccg
     5761 acgtttttac gcaaggaggg accggccgat acgatcacgc cgggtattcc cgaaaacgcg
     5821 ccgaacagat catgaaccgc gtgcgcccgc tcaagatggc gaaggttgca caggatgttc
     5881 tcgacccagc gggcctcgaa ctcgaagcga gtgagcagat cgctcagctt ggacgaaagg
     5941 tactggttga tgtcgcgcat ggccgcgtcg tagaattcgg catcgatgag gtaggacgga
     6001 cgatgcgtgt aaaaccgcgc gccgtggaag cgggccatgt cgagcgattc gatgtagccg
     6061 gaaaggtcgc gcggggttgt gacgacgatc gcaccagcca gggatgacgg attgtgcgtg
     6121 tcgcacagat cgacgacctc gcggtcgcgc tcgaccacca gcaggtcgca ttccgggaag
     6181 cgctcgcgca ggagcgtcac atggtagccg aggccgcatc cgagaacgac gatgaggttc
     6241 gcgcgcccga cagaaaacga ttggaccgag cgcgacgcct cgcggacagg cccataggcg
     6301 gagtgaagac ggatttcgcg atctcgtgtg aagatgaggg ttttcgtgcc ttcgggcgta
     6361 tcctggaccg cgtatgttcc catgaggaag aggcgcgaac cctactgctc ctttgcggtg
     6421 ttgttggcga gcatggtctt gaactcttca tggaacttgc caaaggcctc ggggccgtta
     6481 attttgaagc gttcaaggac gtcctcgtcg aggtagtaat acccgtactt gcgcgactcg
     6541 atgccgcaca tcatgttggc caggtaggtg gtgtatacca ggtcgcggta ttcctcggag
     6601 gcgctcagcg gggaatggtg atatttaatc gcctcgatga gatactcggg gaactgccac
     6661 tttcgcgcga tgagctcgcc gattgacgaa tggctgatgc cgatggacac ctcctccatg
     6721 acggtggaaa cgcgcatctt ccggtccttg acgatgtccg atatccagtt ggtgatcttc
     6781 gtgtcggtcg acaggaggac gatcttcccg agatcgtgca ggagccccgc gaggaacgaa
     6841 tgctcgagta tggcgcccat cttgtaccgc tgcgcgatat ttcgcgcgta gaaggccgtt
     6901 ttgttgcagt gcgtccagat ctgctcgaat ttcgagaagc gcttgtcgag tatgcgtcgt
     6961 gcgctggcgg cgacaaggat cgagttgagg ttcttgaggc cgatcttgat gagcgcctcg
     7021 ttcacgttgt cgatgcgccg ctgggtgagg aagcccgccg agttcgacag cttcagcacg
     7081 tcggtggtga gggccggatc gagcatgatc ttcttcgcga tatcgtcgat gtccaccgtg
     7141 ctgtccttgc acatcgcctg gagctcgatg atgttcttgg gaaaggtggg caggccgttg
     7201 acctcgttga tgatctgctt tttaatctcg gtcgttatct cgttcggacg ggtctgtccg
     7261 ggtatgacga tccacgacca ggtcttcttt ccatcggaat tgagcacata cgatgatgtg
     7321 ttgatcccgg agtttttcag cagcagcatg atgaggacga tgcccaaccc cgcgccctcc
     7381 gtgctgtcgt atatctcgtc gtaggcgtcg gtgaagtcgt tgtattcgat cgccttcgcc
     7441 ttgcgcaggt tgatgcgctc gagctcctcc ggaaggatcg ggacgtcatt ggtgaccgtg
     7501 atcttgaagc cgccgttttc cttgttgatg ttcatggata ccttgaggtc cgacttcttc
     7561 atgtcctcgg ccagctcctc aaggttcccg atcacgttgt ttttgaaatt ctggatgccc
     7621 tcggtatact catttggctt ggtgatgtcg agattgatct tcttgaagta cacgcgtttc
     7681 gcattcgcct tcgtcgcgtt gatgatgatt tcgcggagaa tggtcgtgac cgtgtcgaga
     7741 agaaatattt ggtcgaagtt tgcgagaagc tttgcaagga gggaattgat cgactgcatt
     7801 atttcgccgt gggggtagta gaagttgaat tctaccggct ctccgccatt gacggtggtt
     7861 accatttttg tcgtatttaa tatcgccatg actctatgtg tacacttttg taatttcctt
     7921 gggcatggta tatggtatgc ctctgatcgc aattttgtca atatagactt tttttcaatg
     7981 ttccttcatg cgcattattg ttgattcagg atcgagcatc gaggagcgtc ggggcctgcg
     8041 gagatcatcg tcgcgaacgc gagcgcgcca atccgggcaa gatgggaatc gagggcgagg
     8101 acaagatcga ttggcggaac ggacgacgac gacgcgaaga gccgatcacg ggcggtttcc
     8161 ggtcgggcgc ctgatcctga tcgcgacgga atgatcttga gcggtacgcg atcggcgtcg
     8221 agcgcatggc agagacgtcg gaaggcgacg ttgatgcgaa agtcgtcgct tacgtgaacg
     8281 ctcgcgatct tttggcgctt gatgatgtgc acgaaataac ggacgctggc ataatcggcc
     8341 ttcgtgatcg cgatatgggt ctccgatggt tgagatgcgg tcagcgctgc atcgaccgcc
     8401 ctcgcgtcct caaagcttcc gaggtgtccg gcaaccacgg caacacgggg ccccgtgaat
     8461 acgacagcct gcgaatgcgt gtttttcaaa accgaaagcg atggcccggt tgggggctgc
     8521 accgaagcgc aatacagcca tgcgccaacg taagcgagcg cgagcgacga tggcgcgact
     8581 atccaaaagc gtcgtatggg cgcaagtgtc aatacgagaa acacatacgg gatcagaagc
     8641 aacggcgccg gctcgttgat tataaaatgc cccggtatat gtgcgcacgt ccgtataaac
     8701 tgatggttac agtcgaacaa ccgctcgatc gcaggcgcga gaagatgcgc cagcggcggc
     8761 gagagtgcgg aaagtgcggt caccgcgatc gaaccgatca gcatcacgct catccccggt
     8821 accgcgataa gattggtgat aatgcccacg agattgagct cgtgcacctg caagagtaaa
     8881 atcggataga cgggaatctg ggcggcgcag gtgacgctga gcgatgatgc tatcggaccc
     8941 ggaagagcac gaaaggccgt tgagagcggt ctggcgaaca ggagtatgcc cagggtggcc
     9001 ccgaaggaca gttggaatcc cagaccgtac agctcccacg gcgcgacgat caggatcacg
     9061 accgcagacc acagcagggt attgatgatg ttccggtcga ggtcgaacag tttttgaatc
     9121 gtgtagatcg cgaacatgac gtaggcgcga aagagcgaca ccgggacatc ggtgagcgcg
     9181 aggtatccgc tgaccgcgac gagggtcgcc gccatcgccg ctttgcggtc ggtgcgcagc
     9241 gcgccgaaaa acgcaagcgg aatcagggca atgagaccca cgttcatgcc gctggcggct
     9301 aggatatgga gcacgccagc gcgtcgaaat tcgtcctgca cgcgcttggt gatcacgtgt
     9361 tgattcccga agtacagcgc ctcgatcatt cgggcggttt cgggcctata gagccggtcg
     9421 agcgcctcct cgatgcgttt tcgaacgtcg cgacgaagcg acggcgatgt tttttcaacg
     9481 acggatatcg ttttctcatc gagataaaaa accgccgaga ccccctttcg gagccgatcg
     9541 acggcccatc catcagtggg gccgttttgc cgttgaatcg cgcgcgggaa agcgctgatc
     9601 cggacgcggt cgcccgacgc gagttcgccg ggacggcgaa ttatgccgag cgcttgccga
     9661 gaggcggcaa tctgcagcga gccgtccggt ataagcgtga ggtgcgtgtc gtagcgtcgt
     9721 tcctggatgt cggcgatgca atagatgcgc gcgggatcgt gcgggaaatc gcctgcgggc
     9781 aggtggatga cgcgcgccag gacggttgcc ccgagggcga gcgggatcag cacatgccgg
     9841 acgatggaaa aatccttcgt ttgcatgtgt cgatacagag cgagcgcagc aagtagtaca
     9901 atgacaagcg ccagcccgag gtatgtgtcg gatgtgattg cgagcgcatt gccatagccg
     9961 atcgcgatgc cggcgcacag cgctatcagg ctgaaaaatg atggcgtgat gagattgcgt
    10021 agatttgtac gatgaagcga gataagttcg tttatttcat tcctgcgggc tgtttcacga
    10081 atgttttttt ggagcgcggc gatgtgttgg gttgattgtg atgatgctgt gggtcgctta
    10141 aagcctgccc acattgtaca tgactcctat gcggccgcca aacgatgtta tggtcgattc
    10201 ctcgccaaag ccgaaacgtc cggtacgagc aatgccatcg acaaaaattt ttacatccgt
    10261 acccaggttg aacacaaggc cgatcgatcc cgcgaatccg gcgccaccgg tgtagtcttt
    10321 tttaaacggg gggatctcga gatacatgcc cactccgccg gcgacgccga cgacatcgaa
    10381 gaagaaccag gcgaactttc cgagcgcggt ggccattgta taactcgccg agacgtccgg
    10441 ggtgccggca ttggcgctgc tgatatactg gagcgagaac tcgcc
//
LOCUS       DAORHF010000123        10464 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5636_length_10464_cov_6.224037, whole genome shotgun sequence.
ACCESSION   DAORHF010000123 DAORHF010000000
VERSION     DAORHF010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_10464_cov_6.224037"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="PKJ16_11025"
     CDS             <1..874
                     /locus_tag="PKJ16_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNV47564.1"
                     /translation="SYSDKCLRVGVRAGDLLDESLLRERLSQTVRMKNLQLEKIYGKP
                     VMQAEAIIADLLRYRDEIGGMITNTPYLLHAALHSGKRVLLEGAQGNMLDIDHGTYPY
                     VTSSNPTIGGALMGSGLNAFDITEVVGITKAYVTRVGEGPFPTEDHCEAGALLRERGG
                     EFGATTGRPRRCGWFDAELMKHAVRINGLSRIVLTKLDVLSGFKRIKAAVAYERNGRR
                     LDFAPTAHLDDVVPVYEEFDGWDEDISTCTDYAALPARARDYVEFIEKTLGVRISIIS
                     VGPDRDNTLVREQA"
     gene            944..1016
                     /locus_tag="PKJ16_11030"
     tRNA            944..1016
                     /locus_tag="PKJ16_11030"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:977..979,aa:Lys,seq:ttt)
     gene            1020..1092
                     /locus_tag="PKJ16_11035"
     tRNA            1020..1092
                     /locus_tag="PKJ16_11035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1054..1056,aa:Glu,seq:ttc)
     gene            1257..1604
                     /locus_tag="PKJ16_11040"
     CDS             1257..1604
                     /locus_tag="PKJ16_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47565.1"
                     /translation="MARRALFITALVLIGLGLIAQNKADFTGTFNMDNGKTKMVLVQK
                     GDRVTGDYSGQDNGKLEGTVVQGKLTYTWIQTTGEKGKGWFTLSSDGKSISGRWGNGA
                     SDTDGGDWKGVRN"
     gene            complement(1621..2328)
                     /locus_tag="PKJ16_11045"
     CDS             complement(1621..2328)
                     /locus_tag="PKJ16_11045"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV47566.1"
                     /translation="MARRAAIFACLLIAFAAAPAAAQRMGRIVDITGDADIVSLNTGG
                     KLAPAVGTVIMRDYRVRTGAKSTMEIQLDDGTKVIMRETTVLSVLAVKTREQDPPARI
                     RLLGGTARVISASRFRSRALVIKAPTAIVGIMGGRATDVGMVASAIDARVVVFKGAID
                     AASSVERIIKSYAVGKGEEVRIAKNSQPEPPRKVPEKVFNAWFETYYIDENDAIVRID
                     PNADRSILDSLLRKKRY"
     gene            complement(2329..4629)
                     /locus_tag="PKJ16_11050"
     CDS             complement(2329..4629)
                     /locus_tag="PKJ16_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HNV47567.1"
                     /translation="MIRIRQLSLVFILSLVSCLGPSPHDASLAALGLYRTPEEVVERL
                     KGDPKSYREHFMLGMAYKKLKKYKTSIHHFANSCFAYSQNKNLKIFARPVYRYVDEIH
                     IKTEFYDDAVFHIADQFYLYREYEHVVTFIDLMKKRPTALYRDAVMLKSRALLELSRP
                     QAAIDVLADLERLYEDPISKAAIAIRRASVHEKMNQPERAVQAYLAAIAVDERGWHAT
                     IAVNQLATLTKNFTYQYRDAELLSLGKAFHHNGKYDAAAGLITALRERKPADTLSHQA
                     NASMIRTLVRLRRFADADAIVRSYEGDPERKRAMLKEKADELWEMRLRREAADAYEAL
                     AAGHDDDLAQEALNRVCRNAEKSKRAGWEAALRRFVERFPKNASSEYLLWLLARDELK
                     KRNYAQAREHLAKANNLFPAGQFSDRIRFWLFKLYALDRKNDEALRFARELVSRNPGS
                     PYAWILLERHSKDHTASGLSAAFDAARAKGDRDGAAYYHGLLYFKERDRDRRDRRAEQ
                     FFSAFDNPAAEFEKHVSSMSLASKYAGALRDCERYFQTGAMEALNRELDLLPADERTR
                     RDRYTALSHFGAKYGNWYLMVSATLELMKLTGASENIMLFSRAAVDRLMPLAYVDCID
                     SAAKRFRVDRLLLAAIIRSESLFNHAAVSSAGATGLMQLMPQTARGLARELRLARYDL
                     KDPCTSVDLGARYLQWLAANFRNNIDDMMGAYNAGPGMINKWRASLRADDGDFYVELI
                     PFQETRFYILRSRKHLLQYQVLYGAR"
     gene            complement(4640..5551)
                     /locus_tag="PKJ16_11055"
     CDS             complement(4640..5551)
                     /locus_tag="PKJ16_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47568.1"
                     /translation="MINREKSRNIPRALARRIIRWGLAALAIACIAGPAAAQRTKSSL
                     RAGEQVKAEKGLKDNRYFFYFINASITNFGTDDEKKAFTEAVRRDILAQFLYMRFMFY
                     ESYTEIRHSQKILIDLYRKVLIKDIAMSKELLNRFAPAVVEVKDGSALEYLHLGYRDM
                     KVAEQYLLMADNFRRDLYSMRLYKYVHAIKTAKHGKKYAFLGALLARDMDNVFKRKRT
                     ERMAQFDLEQLVRKTKLAIRQLSFDALSKRIVEIPPPEQTAAYQTMHLDSVYRSREPK
                     SLYDAIWEEPRVSELPEYKRYIVGPEN"
     gene            5655..6653
                     /locus_tag="PKJ16_11060"
     CDS             5655..6653
                     /locus_tag="PKJ16_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47569.1"
                     /translation="MNYALSALALVACLFVAACGSIPLDPSLLNKRPEAELEKVEIDA
                     ISLRDVTFLFRIAVKNPFPLGLSLAGVRMRFAVENNQLFATETRGGLNVPAKGKESTT
                     FLVTVKYEDIEKAARNYMARESLDCAIAGEIVVKLPTGGIKGLPGTWEFPFTFSKKIP
                     TIKPVVRIVNFKVTQPSRTDIEKDLRRIGKETLSPDKIAGLFGDMLSGKKKQSLSEVV
                     PADLDVKFGVSFSIELENKTPAKIAFRTLDYGFAINNAKLIDGTTKSIVSTGNKSVLN
                     ISNQFSSRALGDSIVKAFMDRKGAFTFRGRTSLQLPAGIKKEPVRLDFNESGTLSF"
     gene            6792..7496
                     /locus_tag="PKJ16_11065"
     CDS             6792..7496
                     /locus_tag="PKJ16_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47570.1"
                     /translation="MKINVDLSEVVPVRGANYFQSRRAPSTVMPPAAGASEGGTINPL
                     AANAGNERRLSQAMTIALNASALISKALVAAARLRTMAQDSITGGNVDYREMSGTITD
                     IRSSLSAAGMAYAAPAIAAYTAPDGAKLPAIKDELAVIGTAADALQGARAADLPALDG
                     AIAGLTAKQGAVDAVAARLGRSVDAAMGQYPGYTGAVDYGRLVRSVTGDIAGNPQLAL
                     AAQGNLNPEKVAGLSA"
     gene            7660..8064
                     /locus_tag="PKJ16_11070"
     CDS             7660..8064
                     /locus_tag="PKJ16_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47571.1"
                     /translation="MEVFDGFIAAIAAEFKIKFHKDAENVYTTTIEFENDRTQEVLIT
                     LSKDEAGDRIINYYSIVVKLKTDLCELYKYALQVNSSLHYGALALLDNTLIIRNSIML
                     HHCEPDRFMKSLTYIAAKADELEELLIRENIH"
     gene            8090..8503
                     /locus_tag="PKJ16_11075"
     CDS             8090..8503
                     /locus_tag="PKJ16_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47572.1"
                     /translation="MARHIIAGIALVAFLVSGCDSLGKYGEAKRALNDFAEAMDAYSA
                     ALEKSATPADVVAATDAYTKRLEVIMPKIRELEKKHPESRNQSEPPAELKDVVARVQE
                     SQKRMVKNSVDIFLMKYPSDAEVQKANMRLVEVLK"
     gene            8681..10108
                     /locus_tag="PKJ16_11080"
     CDS             8681..10108
                     /locus_tag="PKJ16_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV47573.1"
                     /translation="MNLESFFNPKSVAVVGASNTPFNLAATICNILKYKSYHGAVYAV
                     NRKAEEVHGCPGYASVKDIPGEVELAVILISAKQVPGVVRECGEKGVRGVIIESAGFS
                     EAGPEGQAMQDEIDGYLKKYNMRMLGPNCLGVLNAANSFCCFYGIIPGRYGDGFSTPG
                     TVSYIIQSGGIGALVLDSFNKDVCKVNKAVSIGNMADVDESDLIEYFNGDGTEVIGLY
                     LENVRNGRKLMDTARRVTKPILAFKVGRTSEGSAAAASHTAGMANNDIVFDNACRQAG
                     IIRLKSISELHAMPKMFTTMPLLRGKNIAVMSNSGAFGGIAADLLIESGFSMAKLTPA
                     TQERLAKTGQLFNVKNPVDLGPTLNKQTFVDIFDILLSADEVDGLLPVPNVWLDVVIE
                     AILELMAMCRHYNKPAAIYIPNAIDRILTIREKHGIPTFESPEEATRALVVSHQQYRY
                     LQKKETGNHENDLNRRAKPEAACAV"
     gene            10056..>10464
                     /locus_tag="PKJ16_11085"
     CDS             10056..>10464
                     /locus_tag="PKJ16_11085"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV47574.1"
                     /translation="MKTILTDARSRKQRALSEYDSKRVIAAAGVPMPREELVASKDDA
                     LRAAAAIGYPVVLKGCADTLLHKTEKNMVFLNLASPDDVGDAYDRITGAGEPLDGVLV
                     QEQVRGGRELVIGMVRDPQFGPCVMFGLGDVFTE"
BASE COUNT         1960 a         3227 c         3373 g         1904 t
ORIGIN      
        1 atcgtactcc gacaagtgcc tgcgcgtggg cgttcgcgcc ggcgacctcc tcgacgagtc
       61 gctgctccgg gagcgcctct cgcagacggt ccggatgaag aacctccagc tcgagaagat
      121 ctacgggaag cccgtgatgc aggcggaggc catcatcgcc gacctgctgc gctatcgcga
      181 cgagatcggc ggcatgatca cgaacacccc ctacctgctg catgcggcgc ttcacagcgg
      241 caagcgcgta ctcctcgagg gcgcgcaggg gaacatgctg gacatcgacc acgggaccta
      301 cccgtacgtc acctcctcca acccgaccat cggcggcgcg ctcatgggct cgggcctcaa
      361 cgccttcgac atcaccgagg tcgtggggat caccaaggcc tatgtgacgc gcgttggcga
      421 ggggcccttc ccgaccgagg accactgcga ggcgggcgcg ctcctgcgtg agcgcggcgg
      481 cgagttcggc gcgaccaccg ggcgcccgcg ccgctgcggc tggttcgacg cggagctgat
      541 gaagcacgcc gtgcgcatca acggtctctc gcgcatcgtg ctcaccaagc tggacgtact
      601 ctccgggttc aagagaatca aggcggcggt cgcctacgag cggaacggcc gccggctcga
      661 cttcgccccg acggcgcatc tcgatgacgt tgtccccgtc tacgaggagt tcgacggctg
      721 ggacgaggac atctcgacct gcacggacta tgccgcgctg cccgcacgcg cgcgcgacta
      781 tgtcgaattc atcgagaaga cgctgggggt gcggatctcc atcatttcgg tgggccccga
      841 ccgcgacaat acgctggtgc gcgaacaggc gtaatcgaga tgcggcgatt aaaaaaaaat
      901 ttgacagcaa ttgacgcatc tttataaggg ttcaaatctg ccggagccgc tagctcagtc
      961 ggtagagcaa ctgcctttta agcagtgggt cgggagttca attctcccgc ggctcacatg
     1021 tccccgtcgt ctaggggtct aggacaccgg gttttcatcc cggcagcagg ggttcgaatc
     1081 ccctcgggga tgcatggcga agggcgatta tccgatcggg taatcgccct tcgcttttat
     1141 atgccgtcag cgacgcctcc gacgcgcatc gcggatcgat atttggcttg acgtttccgc
     1201 gaaacgcggg catgatatcg catacagaaa tattggtact gtacggggag gtcgtcatgg
     1261 ctcgacgggc attgttcatc accgcgctgg tcctcatcgg cctggggctt atcgcgcaaa
     1321 acaaggcgga tttcaccggc accttcaaca tggacaacgg aaaaaccaaa atggtgcttg
     1381 tccaaaaggg ggaccgcgtc accggcgatt attcgggaca ggacaacgga aagctcgagg
     1441 gaacggtggt ccaggggaag ctcacgtaca cctggatcca aaccaccggc gagaagggca
     1501 agggctggtt cacgctatcg tccgacggaa agtcgatatc cggacgctgg ggaaacggcg
     1561 catcggatac ggacgggggc gattggaagg gcgtgcggaa ctgacacctc cccagcgcgc
     1621 tcagtaccgt tttttccgca ggagcgagtc caggatactg cggtccgcat tcggatcgat
     1681 ccgcacgatc gcgtcgttct cgtcgatata gtacgtctcg aaccatgcgt tgaacacctt
     1741 ctccgggacc ttacggggcg gctccggctg gctgtttttc gcgatgcgca cctcctcccc
     1801 cttcccgacc gcatacgatt taatgatgcg ctccaccgag ctcgcagcgt cgattgcacc
     1861 tttaaagacg accacgcgcg cgtcgatcgc gctggccacc atcccaacgt cggtcgcccg
     1921 tccccccatg atgcccacga tcgcggtggg cgcttttatc acgagcgccc gtgaccgaaa
     1981 gcgcgaagcg ctgatgacgc gcgccgtacc gccgaggagc cggatgcgcg cgggcgggtc
     2041 ctgctcgcgc gtcttgacgg ccagcacgct gagcaccgtc gtctcgcgca tgatgacctt
     2101 ggtcccgtcg tcgagttgga tctccatcgt cgatttcgcg cccgtcctga cgcggtagtc
     2161 gcgcatgatg accgtcccca ccgccggcgc gagcttcccg cctgtgttga gcgaaacgat
     2221 gtccgcgtcg ccggtgatgt ccacgatgcg gcccatgcgc tgcgcggcgg ccggcgcggc
     2281 cgcgaacgcg atgagaaggc acgcgaaaat tgccgcacgc cgcgccatct accgcgctcc
     2341 gtagaggacc tggtactgca gcaggtgctt gcgcgagcgc aggatataga agcgcgtctc
     2401 ctgaaagggg atgagctcga cgtagaagtc gccgtcgtcg gcccgcagcg aggcgcgcca
     2461 cttgttgatc atcccgggcc cggcattgta ggcgcccatc atgtcgtcga tgttgttgcg
     2521 gaaattcgcg gcgagccatt gcaggtagcg cgcgccgagg tccaccgagg tgcagggatc
     2581 cttgaggtcg tagcgggcga gccgaagctc gcgcgcgagc ccgcgggccg tttgcggcat
     2641 aagctgcatg agccccgtcg cgccggcgga ggacacggcg gcgtggttga acagcgactc
     2701 ggagcggatg atggccgcga gcagcagtcg gtccacccgg aagcgcttcg cggcgctgtc
     2761 gatgcagtcg acataggcca gcggcatgag gcggtcgacc gccgcgcgcg agaagagcat
     2821 gatgttctcg ctggcgcccg tgagcttcat gagctccagg gtggcgctca ccatgaggta
     2881 ccagttcccg tatttcgcgc cgaagtgcga gagggccgtg taccggtcgc gccgggtccg
     2941 ctcgtcggcg ggcaaaaggt ccagctcgcg gttgagcgcc tccattgcgc ccgtctggaa
     3001 atatctttcg cagtcgcgca gcgcgcccgc gtatttcgag gcgaggctca tcgaggagac
     3061 gtgcttctcg aactccgcgg cgggattgtc gaaggccgaa aagaactgtt cggcgcgacg
     3121 gtcgcggcga tcgcgatcgc gctccttgaa atacaggagg ccgtgataat aggcggcgcc
     3181 gtcgcgatcg cccttcgcac gcgccgcgtc gaacgccgcg gagaggccgc tcgcagtatg
     3241 gtctttcgaa tgccgctcca gaaggatcca ggcatagggc gatccggggt tgcgcgatac
     3301 cagctcgcgt gcgaagcgaa gcgcctcgtc gtttttccgg tcgagggcgt aaagcttgaa
     3361 gagccagaag cggatgcggt cggagaactg gcccgcgggg aagaggttgt tcgccttcgc
     3421 caggtgctcc cgcgcctgcg cgtagttccg cttcttgagc tcgtcgcgcg ccaggagcca
     3481 gaggaggtat tcgctcgagg cgtttttcgg gaagcgttcg acgaagcgcc gaagggccgc
     3541 ctcccagccg gcgcgcttgc tcttctcggc attgcggcac acgcgattga gcgcctcctg
     3601 cgcgaggtcg tcgtcgtgcc cggcggcgag ggcctcgtag gcgtcggcgg cctcgcggcg
     3661 caagcgcatc tcccacagct cgtcggcctt ctccttgagc atggcgcgct tccgctcggg
     3721 atcgccctcg taggagcgca cgatggcgtc ggcgtcggcg aagcgcctga gccgcaccag
     3781 ggtgcggatc atactggcgt tcgcctggtg tgacagcgtg tccgccggtt tccgctcgcg
     3841 cagcgcggtg atgagccccg cggccgcatc gtacttgccg ttgtgatgga aggccttccc
     3901 cagcgacaag agctccgcat cgcgatactg gtaggtgaaa ttcttcgtga gggtcgcgag
     3961 ctggttgacg gcgatggtcg cgtgccagcc gcgctcgtcg acggcgatgg ccgcgagata
     4021 ggcctgcacc gcgcgctccg gctggttcat cttctcatgg accgaggcgc gccggatcgc
     4081 gatggccgcc ttcgagatgg ggtcctcgta gagccgctcg agatcggcga ggacgtcgat
     4141 ggccgcctgc gggcgcgaga gctcaaggag cgcgcgcgat ttgagcatca cggcgtcgcg
     4201 gtacagggcg gtggggcgct tcttcatcag gtcgatgaac gtgaccacgt gctcgtattc
     4261 gcggtagaga taaaactggt cggcgatatg gaacacggcg tcgtcgtaga actccgtctt
     4321 gatgtggatc tcgtcgacgt agcggtacac gggacgcgcg aatatcttca ggttcttgtt
     4381 ctgcgaatac gcgaaacagc tgttcgcgaa atggtggatc gacgtcttgt acttcttgag
     4441 cttcttgtag gccatcccca gcatgaaatg ctcgcggtag gatttcggat cgcccttgag
     4501 gcgctcgacg acctcctccg gggtgcggta gagtcccagc gccgcgagcg aggcgtcgtg
     4561 cggcgaggga ccgaggcacg acacgagcga aaggatgaac acgagcgaga gttggcggat
     4621 gcggatcatg agaagtctcc taattttcgg ggccgacgat gtaccgcttg tattcgggga
     4681 gctcgctcac ccgcggctcc tcccatatcg cgtcgtaaag tgatttcggc tcgcgggacc
     4741 ggtagacgct atccaggtgc atggtctggt aggcggcggt ctgttcgggc ggcgggatct
     4801 ccacgatccg cttggaaagg gcgtcaaagg agagctgccg gatggcgagc ttcgttttgc
     4861 gcaccagctg ttcgaggtcg aactgcgcca tccgctccgt gcgttttcgc ttgaagacat
     4921 tgtccatgtc acgggccagg agcgcgccga ggaaggcgta tttcttcccg tgtttcgcgg
     4981 ttttgatggc gtgcacgtac ttgtacagcc gcatcgaata gaggtcccgg cggaagttgt
     5041 cggccatcag gaggtattgc tcggccacct tcatgtcgcg atagcccaga tgcaggtact
     5101 ccagggcgga tccgtccttc acctccacca ccgcgggggc gaagcggttg aggagctcct
     5161 tggacatggc gatgtccttg atgagcacct tccggtagag gtcgatgagg atcttctgcg
     5221 agtggcggat ctccgtgtac gattcgtaga acatgaagcg catgtacagg aactgcgcca
     5281 ggatgtcgcg gcgcacggcc tccgtaaagg ccttcttctc gtcgtccgtg ccgaagttgg
     5341 tgatggaggc gttgatgaaa tagaagaagt accggttgtc cttgagcccc ttctcggcct
     5401 tgacctgctc gccggcgcgc aggctcgact tggtccgctg cgccgccgcg gggccggcga
     5461 tgcacgcgat cgccagggcg gcaagccccc agcggatgat ccggcgcgcg agcgcgcgcg
     5521 gtatgtttct tgacttttcc cgatttatca tcgcctgctg gtcctatgaa tgtgtggcaa
     5581 aggtccggct tttcgtcaaa ccttttccgc cggtttcccc gcgccgggcc gccatcaacc
     5641 ctggaggatc aaccatgaac tacgcactaa gcgcgctcgc cctcgtcgcc tgccttttcg
     5701 tcgccgcctg cgggagcatc ccgctggacc cgtcgttgtt gaacaagcgc cccgaggccg
     5761 agctcgagaa agtcgagatc gacgcaatta gccttcgcga cgtcaccttc ctgtttcgca
     5821 tcgccgtgaa aaacccgttc cctctgggcc tgtcgctggc cggggtgcgg atgcgcttcg
     5881 cggtggagaa caaccagctc ttcgcgaccg aaacgcgggg cgggctcaat gtgccggcga
     5941 aggggaagga gtcgaccacc ttcctggtga ccgtgaaata cgaggacatc gaaaaggccg
     6001 cgcgcaacta catggcccgg gaatcgctcg actgcgcgat cgcgggcgag attgtcgtga
     6061 agctgcccac cggcggcatc aaggggcttc ccgggacctg ggagtttccg tttaccttct
     6121 ccaagaagat ccccaccatc aagccggtgg tgcgcatcgt caatttcaag gtgacgcagc
     6181 cctcgcggac ggacatcgag aaggacctca ggcgcatcgg gaaagagacc ctgagccccg
     6241 acaaaatcgc gggcctgttc ggcgatatgc tgtcgggtaa aaagaagcaa tccctgagcg
     6301 aggtggtgcc cgcggacctt gacgtgaagt ttggcgtgag cttctccatc gagctggaga
     6361 acaagacccc cgcgaaaatc gccttcagga cgctggatta cggatttgcc atcaacaatg
     6421 cgaaattgat cgacgggacc acgaagagca tcgtttcgac cgggaacaag tcggtgctca
     6481 acatcagcaa ccagttcagc tcgcgcgcgc tgggcgattc tatcgtaaag gcgttcatgg
     6541 accggaaggg ggcctttacc ttccggggca gaacgtcgct gcaactgccc gcggggatca
     6601 aaaaggagcc cgtgcggctc gatttcaacg aatcggggac gctctccttc taactttcta
     6661 ttgacagcga cgggcgcgtc atggtatatt gatggcacgg taacggattc taccgccgcc
     6721 gctcccccgg gcaggggcgc ggcgtttccg cgctcgcgcg ggcgaagggt cataatccag
     6781 ggagggagat tatgaaaatc aacgtcgatc tcagcgaggt cgtccccgtc cggggggcga
     6841 attatttcca atcacggagg gcgccgtcga ccgtcatgcc gccggcggca ggcgcgtccg
     6901 agggcggcac catcaatccg ctcgcggcga atgcgggcaa cgagcgtcgg ctgtcgcagg
     6961 cgatgacgat cgcgctcaac gccagcgcgc ttatcagcaa ggcgctggtc gccgcggcgc
     7021 gcctcaggac catggcgcag gattccatca cgggcgggaa cgtcgattat cgcgagatga
     7081 gcggcacgat caccgacatc cgctcgtcgc tctccgctgc cggcatggcc tatgcggccc
     7141 cggccatcgc cgcctatacc gcccccgacg gcgcgaaact cccggcgatc aaggacgagc
     7201 ttgccgtgat cggcactgcg gcggatgcgc tgcagggcgc acgcgccgcg gacctgccgg
     7261 cgctcgacgg cgcgatcgcg ggcctcacgg caaagcaggg cgcggtcgat gccgtcgccg
     7321 cgcgcctcgg gcggtcggtc gatgcggcga tggggcagta tccgggctac acgggcgcgg
     7381 tcgattacgg ccgcctggtc aggtccgtga cgggcgacat cgcgggcaat ccgcagctcg
     7441 ccctggccgc gcagggaaat ctcaatcccg agaaggtcgc gggcctgtcc gcctgacgtc
     7501 tctcccggcg cgcgccggct ctcgatgggc gcgctccggg aaaaatcccc acgcatcgga
     7561 aaaatacctt cttgactttt tcccgcgtgt ttgcgcacct caatatcagc ggcgcggcgg
     7621 atgcgcgcgc cgggacatca gcgaaaacga gaagcgggca tggaagtatt tgacgggttt
     7681 atcgcggcga tcgcggcgga gttcaagatc aagtttcaca aggacgccga gaacgtctat
     7741 accacgacca tcgagttcga aaacgaccgg acccaggagg tcctcatcac cctgtcgaag
     7801 gacgaggccg gcgaccggat catcaattat tactcgatcg tggtcaaact caagaccgac
     7861 ctgtgcgagc tctacaagta cgccctgcag gtgaacagct cgctccatta cggggcgctg
     7921 gcgctcctgg acaacacgct catcatccgg aactccatca tgctgcacca ctgcgagccc
     7981 gaccgcttca tgaagtcgct cacctatatc gccgcgaagg cggacgagct cgaagagctg
     8041 ctcatccggg aaaacattca ctaagacgca tcgccgaggg ggttcgctca tggcacgaca
     8101 tatcatcgcc ggtatcgcgc ttgtcgcatt tcttgtttcc ggctgcgact cgctgggcaa
     8161 gtacggcgag gcgaaacgcg cgctcaatga tttcgccgag gcgatggacg cctattcggc
     8221 cgccctcgaa aagtccgcga ccccggccga tgtcgtcgcc gcgaccgacg cgtatacgaa
     8281 gcgcctcgag gtgatcatgc cgaaaatccg tgaactggaa aagaaacacc cggaatcgag
     8341 aaaccagtcg gagccgcccg ccgagctcaa ggacgtcgtc gcgcgcgtcc aggagtcgca
     8401 gaagcggatg gtcaagaatt ccgtcgacat cttcctcatg aaatatccgt ccgacgccga
     8461 ggtccagaag gcgaacatgc gtctggtcga ggttctcaag tagcgtcgta ccctgccgtc
     8521 cccgggcgcc ccacgatccc tgaatccatt cgcagaggcc tatcctgcgc cgcatcccgg
     8581 tgcgcgtttt tcgctttttt ttacgaaaag tattgaccaa atagtaacat catttaccaa
     8641 tgtaaacact gtttacttga ccctgcgtgg gaggtgcctc atgaatctgg aaagcttctt
     8701 caatccgaaa tccgtcgccg tcgtgggggc gtcgaatact ccgtttaacc tggcggcgac
     8761 catctgcaat atcctgaaat acaagagcta tcatggggcg gtgtacgccg ttaaccgcaa
     8821 ggcggaggag gtgcacggct gcccgggata tgcgtcggtg aaggacatac ccggcgaggt
     8881 ggagctggcg gtgatcctca tctcggccaa gcaggtaccg ggcgttgtgc gagagtgcgg
     8941 cgagaagggg gtgcgcggcg tcatcatcga gagcgcgggc ttctccgagg cggggcccga
     9001 gggccaggcg atgcaggacg aaatcgacgg ctatctcaag aaatacaaca tgcggatgct
     9061 ggggcccaac tgcctgggcg tgctcaacgc ggccaacagc ttctgctgct tctacggcat
     9121 catccccggc cgttacggcg acggcttctc cacgcccggg acggtctcct acatcatcca
     9181 gagcggcggc atcggggcgc tggttctgga ttcattcaac aaggacgtat gcaaggtgaa
     9241 caaggccgtg agcatcggaa atatggccga tgtggacgag tccgacctca ttgaatactt
     9301 caacggcgac gggaccgagg tgatcgggct gtacctcgag aacgtccgca acgggcgcaa
     9361 gctcatggac acggcgcggc gcgtgaccaa gcccatcctc gccttcaagg tggggcgcac
     9421 cagcgaggga tcggcggcgg cggcctcgca caccgccggg atggcgaaca acgacatcgt
     9481 cttcgacaat gcctgccgcc aggcggggat catacggctg aaatcgataa gcgaactcca
     9541 cgcgatgccc aagatgttca ccaccatgcc ccttctgcgc gggaagaaca tcgcggtcat
     9601 gtccaactcg ggcgccttcg gcgggatcgc ggccgacctg ctcatcgagt ccggattttc
     9661 catggcgaag ctcacgccgg cgacgcagga gcgcctcgcc aagaccggcc agcttttcaa
     9721 tgtaaaaaat ccagtggacc tgggacccac attgaacaag caaaccttcg tcgatatctt
     9781 cgatatcctc ctctcggccg atgaggtgga cggcctgctg ccggttccca atgtgtggct
     9841 ggacgtggtc atcgaggcca tcctggagct catggccatg tgccgccact acaacaagcc
     9901 ggcggcgatc tatatcccca acgccatcga ccgcattctc accatccgcg agaagcacgg
     9961 cattcccacg ttcgagtcgc ccgaggaggc gacgcgggcg ctcgtggtgt cgcaccagca
    10021 gtatcgctat cttcagaaaa aggagacggg taatcatgaa aacgatctta accgacgcgc
    10081 gaagccggaa gcagcgtgcg ctgtctgagt acgattccaa gcgcgtgatc gccgccgcgg
    10141 gcgttcccat gccgcgcgag gagctggtcg cgtcgaagga cgacgcgctc agggcggccg
    10201 ccgcgatcgg ctatcctgtg gtcctcaagg gctgcgccga tacgctgctg cacaaaaccg
    10261 agaagaacat ggtgttcctg aacctcgcga gccccgacga tgtgggcgac gcctacgacc
    10321 gcatcaccgg cgcgggcgag ccgctggacg gcgtgctggt gcaggagcag gtgcgcggcg
    10381 gacgcgagct cgtgattggc atggtgcgcg acccgcagtt cgggccctgc gtgatgtttg
    10441 gcctcgggga cgtcttcacc gagg
//
LOCUS       DAORHF010000124        10455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5649_length_10455_cov_5.541346, whole genome shotgun sequence.
ACCESSION   DAORHF010000124 DAORHF010000000
VERSION     DAORHF010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5649_length_10455_cov_5.541346"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PKJ16_11090"
     CDS             complement(<1..234)
                     /locus_tag="PKJ16_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HNV47575.1"
                     /translation="MACNFILCLHNHQPVGNVDHVLERGYTDSYNKTIDILMNYPEFK
                     FSVHHSGPLLEWIERHHPDYLDKLRVMVDRGQLE"
     gene            complement(374..1594)
                     /gene="glgC"
                     /locus_tag="PKJ16_11095"
     CDS             complement(374..1594)
                     /gene="glgC"
                     /locus_tag="PKJ16_11095"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HNV47576.1"
                     /translation="MITYRFLKKTLTFILAGGQGERLYPLTKDRSKPSVPFGGIYRII
                     DFPLSNAINSDLRKIIVLTQYKSLSLDRHIKTAWNLFTRELEGFVDVVPAQQRIDSNW
                     YRGTADAIFQNIYSIEMEKPEYVLVLSGDHVYKMDYRKLIGFHIEKNADLTIAAIEVP
                     RAEASRFGILEVDAENRVRGFEEKPANPKPIPGKDESSFVSMGIYVFKTERLIRYVSE
                     DHRTEGSNDFGKDIIPRIYSSDRVFAYDYVARESAFNYWRDIGTIKSYYDATLDLVST
                     SPQLNLYDRDWPIRTHMEQYPPAKTVHNDVTRRGVAVESLISSGSIVSGGSVYGSVIS
                     PGVRVNSYAEVYDSIIFSRVNVGRGAKIRNAIIDKNVVIPDNVTIGFDRAEDEKRFTV
                     TEEGIVVIPKSYIF"
     gene            complement(1591..1953)
                     /locus_tag="PKJ16_11100"
     CDS             complement(1591..1953)
                     /locus_tag="PKJ16_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47577.1"
                     /translation="MSTILIVEDEQHQRELYAMELQDEGYTVEQAANGKEAVEMVKKT
                     KYDLVVLDIRMPEMDGIETLGKILSRDKKIPIIIYTAYSNYKSNFMTWTADAYITKSS
                     DMKELKDKIREILAARSK"
     gene            complement(2080..4140)
                     /locus_tag="PKJ16_11105"
     CDS             complement(2080..4140)
                     /locus_tag="PKJ16_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47578.1"
                     /translation="MKLAHYYSKSYTILLLDVCAAMFLIAIITLPAGMLPPGWIRAGI
                     VLTGLYFIARAAVLFFAKREFGASITGLQTIISNFKKGRYSVEVNGDIKNDELGVIYR
                     DLITTGNHINDIMSSQKTEINNLRELYNNIVLSISSYFLVTNDREEVIFANESFCKKF
                     QFDIEEIIGKKIEDLFFFVTGRIKDSLRQIIETREAIVLEKTHILSKNRISVIGDVKL
                     SSIEIKGENQILIVIDDITSKCRKDYQISLISQISETIQRDEEIDTILYTILTGVTSG
                     SGLGFNRAALYLFSDPDQSLAGTMAVGPDTIEEAIEIWNSIQANEYRIAAPRERRDGP
                     AAKGKRFLEKTLSARFDLDAENVFTRALKNMENIHIYDASRDERIDDVVRELMDVNEF
                     VIIPLVAINRPIGIIVADNKFNQVPISNDSIELLSIFAFQAAISIEGYNNIVKLKREM
                     QKIKERQEAMIESEKLAAVGRIAAHIAHEIRNPLVTMGGYAKRIVQQAQKAPKGETIA
                     RAAEVIIKESDRLEKTLSNVMDFTKPSTFIMKFNSVNEVIEDTCSLLHNSFQEKKIGV
                     RLSLLPALPLVKSDFNQLKQVMLNLLQNAIDETPPAGAIEIASAVEAKRIAVYVRNEG
                     SMILEESLSQIFDPFFTTKVTGVGLGLAIVKKIIADHGGEISVRNLPERGVEFKILLP
                     LPGQ"
     gene            4282..4884
                     /locus_tag="PKJ16_11110"
     CDS             4282..4884
                     /locus_tag="PKJ16_11110"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HNV47579.1"
                     /translation="MKLVLATQNAGKIREIKAKLKPLGGIEVLSAAEVPDLPDVVEDG
                     ATFRENALKKSRAIAAHTGLPALADDSGLEVDALNGEPGVYSARYAGEGASDRDRNLL
                     VLDRMRDVPDEKRAARFRCVIAITLPSGEEHVADGVCEGIIARSLSGDGGFGYDPIFF
                     LPEFGKTMAEISVEEKNRISHRGRALDRAAEIIAGLRREA"
     gene            complement(5044..5814)
                     /locus_tag="PKJ16_11115"
     CDS             complement(5044..5814)
                     /locus_tag="PKJ16_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV47580.1"
                     /translation="MSDSLLFEQRGPVGIITINKPKANQLSHDVFEGMRKYLDANEPD
                     KNVRAIVITGAGDKIFCAGADLSSGFGEFGPVDFLKRGQDIWNKIEDYPKPVIAALGG
                     HALGGGCELALACHVRFMKKGARIGLTETNLGIIPGYGGTLRLPRLIGRSRALDCIMF
                     AKQYEAEEALAIGLIDRLCDEGKTLDDAIAYGEEIAKRPPLAIRAVLKIMSASASMSP
                     EQHLKIEREELAKLFSTKDMMEGMTAFMQKRPAVFTGE"
     gene            complement(5864..7030)
                     /locus_tag="PKJ16_11120"
     CDS             complement(5864..7030)
                     /locus_tag="PKJ16_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HNV47581.1"
                     /translation="MNINDVKKIAVIGSGAMGHGIAQVCASAGYSVHMTDIKQEFLDT
                     AMKKVAESLTFLVGKGKITQEAMNAILANNLKTTLDVKEAVSGAQIVIEAVPEVMDLK
                     KSVFKNLSGIAAQDAILATNTSTMSVSEIGSVVSNPSRFIGMHFFNPVTIMKLVEIIY
                     GDKTSDDAVELLCEVSKKCGKIPVKVLKDRPGFIVNRINAPNQALLSAILDEGKMQPD
                     ELDCVMKKMGIKMAPFETADFVGLDVFTHILDYYAQTLSPQYKPGKFLTDKVAKNELG
                     QKSGKGIYDWSSGKAAIDSSKETTDITPLHLLSVQINEAVKVFKEGIATSVEDIDSAV
                     KFGMNAFAGPFTLASGMQPEQLTGALDYLADRFSLDILRPEPEITDGSFKTIGR"
     gene            complement(7248..8444)
                     /locus_tag="PKJ16_11125"
     CDS             complement(7248..8444)
                     /locus_tag="PKJ16_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV47582.1"
                     /translation="MINLETPDYLASMRSSMNNLAAGVLRPIARKYDAAEHEYPAELD
                     MFRGMQILGRPKKKKADAPKPDGGNGKPKKGINLSTVATIEEMCWGDAGLLLSIPNAG
                     LGNAAIAAVATDEQLERFGGKWAAMAITEPGSGSDSGSITTTARRDGGEWVLNGEKIF
                     VTCADRCDIVVVWATVDRSAGKSGIKSFIVEKGTPGFKLEHLEHKLGIRASDTGTFVL
                     DECRIPFDNILGSAEVKKDTEGFKGVMKTFDNTRPMVASMATGIARASLDFTREKLDE
                     SGATIDYKRNLNNVGSLEKDFYLMEAHLEAMRLLVWRAAWMADTEQFNNVEASMAKAK
                     AGRQGTLITQKCCELLGPLGFSKEHLAEKWMRDSKIMDIFEGTGQIQHLIIARNVLEM
                     SGKDLK"
     gene            complement(8653..9750)
                     /locus_tag="PKJ16_11130"
     CDS             complement(8653..9750)
                     /locus_tag="PKJ16_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV47583.1"
                     /translation="MICFACSEEQTLMKETAAKLVQGAIADAAHQMDETGRIPDDAVR
                     KFRELGTLLAAIPEEYGGYGMGNSPIMNSIILEELAAGDMGFAIAATLPALFLMPVMD
                     CGTDAQKARHLADFAADPDGACGTLAINEPRFRFDPYDLATTAVRKNGSYILNGTKCF
                     VPLAETARRIMIAAALDGTNGLFIVDRNNPGLTVGAREKNLGLNSLATHPITLTDCEI
                     PADDRLGGDAGCDYDRLLQRTWTAIAAIGTGICRASYEYAKNYAKEREQFGEAIASRQ
                     SIAFMIAEMAYEVDAMRLMTWKAASALEAGRDAQREAYLARIYAGEMAMKLTDYGVQV
                     LGGHGYIREHPVERYYRNGRGIAILEGMAIA"
     gene            complement(9845..>10455)
                     /locus_tag="PKJ16_11135"
     CDS             complement(9845..>10455)
                     /locus_tag="PKJ16_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47584.1"
                     /translation="AMNAEERKKHILRCAKKLFSRHGFYETQISDIITEADIARGTVY
                     QYFKHKEDIFTTLLEEFYTAWERLIASASENVDLDAVSPEAYLRTRIRTTLLFFAGDN
                     EMCNIVLRMGVGIHKSFDAVIKRFEKKIAGVIIDDLVIGQKYKTVRADIDRELFANII
                     VGALFRVSYYYFVQHKSHKDSRDIDAITDRIVGFISPGLFAK"
BASE COUNT         1744 a         3099 c         3327 g         2285 t
ORIGIN      
        1 ctcgagctgg cctcgatcga ccatgacgcg cagcttgtcg aggtagtcgg gatgatggcg
       61 ctcgatccac tcgaggagcg gtccggaatg gtgcacggaa aacttgaact ccgggtagtt
      121 catcaggatg tcgatggttt tattgtagga gtcggtgtag ccgcgctcca ggacgtggtc
      181 gacgttcccc accggctggt ggttgtgcag gcacaggatg aagttgcagg ccatggtggg
      241 ctcctcgcgg cgtttatcga acgtgaaata ttctcagggg cgtacgcgaa tcacggcaag
      301 aaaaaaatgt ccgatcggag agtatttcgg cgttgggaaa tcgccggtga tgtgccgcgg
      361 cgtgaaggaa cgctcagaaa atataactct tcgggatcac cacgatcccc tcttcggtga
      421 cggtgaatcg cttctcgtcc tcggcccggt cgaaaccgat ggttacgttg tccgggatca
      481 cgacattctt gtcgatgatc gcgttgcgga tcttggcccc ccggccgacg ttcacgcgcg
      541 aaaagatgat ggaatcgtag acctccgcgt agctgttcac gcgaaccccg ggcgagatga
      601 ccgagccgta cacgctcccg ccgctgacga tcgatccgct ggagatgagg gactcgaccg
      661 cgaccccgcg ccgggtcacg tcattgtgca ccgtctttgc gggcgggtat tgctccatgt
      721 gcgttctgat gggccagtcg cggtcgtaca ggttgagctg cgggctggtg gagaccaggt
      781 cgagcgtcgc gtcgtagtac gatttgatgg tgccgatatc gcgccaatag ttgaaggcgc
      841 tctcgcgggc gacatagtcg taggcgaaca cgcggtcgct cgagtatatg cgggggatga
      901 tgtccttgcc gaaatcgttg gacccctccg tgcgatggtc ctcggagacg tagcggatga
      961 gccgttcggt cttgaagacg tatatcccca tggaaacgaa cgatgattcg tcctttccgg
     1021 ggatcggctt cgggttcgcg ggcttctcct cgaagccgcg cacgcggttc tcggcgtcca
     1081 cctccaggat gccgaaacgc gacgcctcgg cgcgcggcac ctcgatcgcg gcgatagtca
     1141 ggtcggcgtt tttctcgatg tggaagccga tcagcttgcg gtagtccatc ttgtacacgt
     1201 ggtcgccgga gaggaccagg acgtactccg gtttctccat ctcgatggag tagatgttct
     1261 ggaagatggc gtcggccgtc ccccggtacc agttgctgtc gatgcgctgc tgcgcgggga
     1321 ccacatcgac gaacccctcg agctcgcggg tgaagaggtt ccacgcggtc ttgatgtgcc
     1381 ggtccagcga gagcgacttg tactgggtga gcacgatgat cttgcgcagg tcggagttga
     1441 tggcgttgct cagggggaaa tcgatgatgc gatagatccc gccgaacggg accgacggct
     1501 tggagcggtc cttggtcagg ggatacagcc gctcgccctg gccgccggcg agaatgaagg
     1561 tcagggtttt tttcaggaac cggtaggtga tcatttcgag cgcgccgcga ggatctccct
     1621 gatcttgtcc ttgagctcct tcatgtcgga cgatttggtg atataggcgt cggccgtcca
     1681 ggtcatgaag ttgctcttgt agttcgagta cgcggtgtag atgatgatgg gaatcttctt
     1741 gtcgcgcgag aggatcttgc ccagcgtctc gatgccgtcc atctcgggca tgcggatgtc
     1801 caggaccacc aggtcgtatt tcgtcttctt gaccatctcg accgcttcct tgccgtttgc
     1861 ggcctgttcg accgtgtagc cttcgtcctg cagctccatg gcgtagagtt cgcgctggtg
     1921 ctgttcgtcc tcgacaatga gtatggtgct cataaccttt cccccgtgcg gtggtgatgc
     1981 ggcgcgcgcg accgtgccac gcccctatac aatatttaat ataacatcga ccgtcgattt
     2041 aatcaatact ttttttccgg cgcgcgccga aaaggcgtct cactggccgg ggagcggcag
     2101 caggatcttg aactcgaccc cccgctccgg aaggttgcgc acggaaattt cgccgccgtg
     2161 gtcggcgatg atcttcttca cgatggccaa ccccagcccc acgccggtca ccttggtagt
     2221 gaagaacggg tcgaaaatct gagagaggct ctcctcgagt atcatggacc cctcgttgcg
     2281 cacatagacg gcgatccgct ttgcctcaac cgcggaggcg atctcgatcg cgccggcggg
     2341 gggcgtctcg tcgatggcgt tctggagcag attgagcatc acctgcttga gctggttgaa
     2401 gtccgatttc accaggggca gggcgggcag cagcgagagg cgcacgccga tcttcttttc
     2461 ctggaaggag ttgtgcagga gcgagcaggt gtcctcgatc acctcgttga cgctgttgaa
     2521 cttcatgatg aaggtcgagg gcttggtgaa gtccatcacg ttggaaagcg tcttctccag
     2581 gcggtcggac tccttgatga tcacctcggc cgcgcgcgcg atcgtctcgc ccttgggggc
     2641 cttctgcgcc tgctggacga tgcgcttggc gtaaccgccc atcgtcacca ggggattgcg
     2701 gatctcgtgc gcgatgtgcg cggcgatgcg ccccacggcc gcgagcttct cggactcgat
     2761 catcgcctcc tgccgctcct tgatcttctg catctcgcgc ttgagcttca cgatgttgtt
     2821 gtaaccctcg atggagatcg ccgcctggaa ggcgaagatg gagaggagct cgatgctgtc
     2881 gttgctgatg ggcacctggt tgaacttgtt gtcggccacg atgatgccga tggggcggtt
     2941 gatggccacc agcgggatga tcacgaactc gttgacgtcc atgagctcgc ggacgacgtc
     3001 gtcgatgcgt tcgtcgcgcg aggcgtcgta gatgtggatg ttctccatgt tcttgagcgc
     3061 gcgcgtgaag acgttttcgg catcgaggtc gaagcgcgcc gagagggtct tctcgaggaa
     3121 gcgcttgccc ttggcggccg ggccgtcgcg gcgctcgcgg ggcgcggcga tccggtactc
     3181 gttggcctgg atcgagttcc agatctcgat ggcctcctcg atggtgtccg ggcccacggc
     3241 catggtcccg gccagcgact ggtcggggtc gctgaacagg tacagcgccg cccggttgaa
     3301 ccccagcccc gatcccgagg tcacgcccgt caggatggtg tagaggatgg tgtcgatctc
     3361 ctcgtcgcgc tggatggtct ccgagatctg gctgatgagc gagatctggt agtccttgcg
     3421 gcatttgctg gtgatgtcgt cgatgacgat gaggatctgg ttttcgccct tgatctcgat
     3481 gctcgagagc ttcacgtcgc cgatcaccga gatgcggttt ttcgagagga tgtgcgtttt
     3541 ttccaggacg atggcctcgc gcgtctcgat gatctggcgg agggagtcct tgatgcgccc
     3601 ggtcacgaaa aaaaagagat cctcgatctt cttgccgatg atctcctcga tgtcgaactg
     3661 gaacttcttg cagaagctct cattggcgaa gatcacctcc tcccggtcgt tggtcaccag
     3721 gaaatacgag ctgatggaca ggacgatatt gttgtagagc tcgcggaggt tgttgatctc
     3781 cgtcttctgc gacgacatga tgtcgttgat gtgattgccg gtggtgatga ggtcgcggta
     3841 aatgacgccc agctcgtcgt tcttgatgtc gccgttcacc tcgaccgagt agcggccctt
     3901 cttgaaattg ctgatgatgg tttgcagccc cgtgatgctt gcgccgaact cgcgcttggc
     3961 gaaaaagagc accgcggcgc gcgcgatgaa gtacaatccg gtgagcacga tgccggcgcg
     4021 tatccatccc ggcgggagca tgcccgccgg gagcgtgatg atggcgatga gaaacatcgc
     4081 cgcgcacacg tccaggagca ggatggtgta ggacttcgag tagtaatggg ccagtttcat
     4141 gttgatgcgt tgacgcgccg ctctatcggg tgtcgatgcc gctattgtat gcgggacgat
     4201 cgtgaaatgc aatcctttca cgcacaataa ttgacttttt ttcgcctgtc gggacgatcg
     4261 tgacgggcga tggggggatt catgaaactg gtgctggcga cgcagaacgc gggcaagatc
     4321 cgcgagatca aggcgaaact caaaccgctg ggcggcatcg aggtgctgtc cgcggccgag
     4381 gtcccggacc tccccgacgt cgtggaggac ggcgcgacct tccgcgagaa cgccctcaag
     4441 aaatcgcgcg cgatcgcggc gcacacgggg cttcccgccc tcgccgacga ctcgggcctc
     4501 gaggtggacg cgctcaacgg cgagcccggg gtgtattccg cgcgctatgc gggcgagggc
     4561 gcgagcgacc gagaccggaa cctcctggtg ctggatcgga tgcgcgacgt cccggacgaa
     4621 aagcgcgcgg cccgcttccg atgcgtcatc gccatcaccc tgccctccgg cgaggagcac
     4681 gtcgccgacg gcgtctgcga gggaataatc gcgcgctctc tctccggcga cggcggtttc
     4741 ggctacgatc ccatcttttt ccttcccgag ttcggaaaga ccatggcgga gatctccgtg
     4801 gaggagaaga accgcatcag ccatcgggga cgggcgctgg accgcgcggc ggagatcatc
     4861 gcgggtttgc gacgcgaagc gtagcatgtc cgacgaggtc gggcgttcct attcgcgcgt
     4921 cgctgtagcc gttaccctgc ggtgccgtct gagagttgct gcgatcgcag aaaaaacggg
     4981 agatgcatcg catccccgaa tcgtcctgaa tggaatgtgt gcactgcgac aatatgcgaa
     5041 acgctattcc ccggtgaaca ccgccgggcg cttctgcatg aaggcggtca tgccctccat
     5101 catgtccttg gtcgagaaga gtttcgccag ctcctcgcgc tcgatcttga ggtgctgctc
     5161 gggcgacatg ctggcgctgg ccgacatgat cttgagcacc gcgcggatcg ccagcggcgg
     5221 gcgcttcgcg atctcctcgc cgtaggcgat cgcgtcgtct aacgtcttcc cctcgtcgca
     5281 caggcggtcg atcagcccga tcgcgagcgc ttcctcagcc tcgtactgct tggcgaacat
     5341 gatgcagtcc agcgcccgcg agcggccgat gagccgcggc agccgcagcg tcccgccgta
     5401 gccggggatg atccccaggt tggtctccgt gaggccgatg cgcgcgccct tcttcatgaa
     5461 gcggacatgg cacgcgagcg cgagctcgca gccgccgccg agcgcgtggc cgcccagcgc
     5521 cgcgatcacc ggtttcgggt agtcctcgat cttgttccag atgtcctggc cgcgcttcag
     5581 gaagtccacg ggcccgaact cgccgaagcc gctggagagg tccgcgccgg cgcagaaaat
     5641 cttgtcgccc gcgccggtga tgacgatcgc ccgcacgttt ttgtcgggct cgttcgcgtc
     5701 gagatacttc cgcatgccct cgaaaacgtc gtggctcagc tggttcgcct tcggcttgtt
     5761 gatggtgatg atgccgaccg gcccccgttg ttcgaacagt aatgaatctg acatgatatc
     5821 ctccgtaagc gttgatccgc ccggcgcgcc gggcgctacg gccctagcgg ccgatcgtct
     5881 tgaacgaacc gtccgttatt tctggctcgg ggcgcaggat gtccagcgaa aagcggtcgg
     5941 ccagataatc gagcgcgccg gtgagctgtt ccggctgcat ccccgaggcg agcgtgaacg
     6001 ggcctgcgaa ggcgttcatt ccgaacttca cggcgctgtc gatgtcctcc acggaggtcg
     6061 cgatgccttc cttgaagacc ttcaccgcct cgttgatctg cacggacagc agatgcagcg
     6121 gggtgatgtc cgtcgtctcc ttcgaggagt cgatcgccgc cttgcccgag gaccagtcgt
     6181 agatgccctt tccggacttc tggcccagct cgttcttcgc gaccttgtcg gtgaggaatt
     6241 tcccgggctt gtactgcggc gagagcgtct gtgcgtagta atcgagtatg tgcgtgaaga
     6301 cgtccaggcc cacgaaatcc gccgtctcga agggcgccat cttgatgccc atcttcttca
     6361 tcacgcagtc gagctcgtcg ggttgcatct ttccctcgtc caggatcgcc gagaggagcg
     6421 cctggttggg ggcgttgatg cggttgacga tgaagccggg ccggtccttg aggaccttga
     6481 ccggaatctt gccgcacttc tttgacacct cgcagaggag ctcaaccgcg tcgtcgctcg
     6541 tcttgtcgcc gtagatgatc tccaccagct tcatgatggt cacgggattg aagaagtgca
     6601 tcccgatgaa gcgcgacggg ttcgatacga ccgagccgat ctccgacacg ctcatcgtcg
     6661 aggtgttggt ggcgagaatc gcgtcctgcg ctgcgatgcc cgaaaggttc ttgaagacgg
     6721 acttcttcag atccatgacc tccggcaccg cctcgatgac gatctgcgcg ccgctcacgg
     6781 cttcctttac gtcgagagtc gtcttcagat tgttcgcgag gatcgcattc atcgcctcct
     6841 gcgtgatctt ccccttgccc acaagaaacg tgaggctttc ggcgaccttc ttcatcgcag
     6901 tgtcgaggaa ctcctgtttg atgtcggtca tgtgcaccga gtagcccgcg ctcgcgcaaa
     6961 cctgggcgat cccatgtccc atggcgcccg atccgatgac ggcgattttt tttacgtcgt
     7021 tgatgttcat tattttctcc tttatctagt ttggctgttg aatgcttgct ggttgtattt
     7081 catcacctca ccccggtcga tcgccattcg ggcctcctgc ccgcgatcga cattagcgcc
     7141 tccgtgcgcg tcacctccgg cgcgccctct cccacaggag agggcttcaa ttattgcgac
     7201 tactgctccc ccttctccta tgggagaagg gggctggggg gatgaggtca cttcaagtct
     7261 ttcccggaca tctcaagcac gttgcgcgcg ataatgaggt gctggatctg ccccgtcccc
     7321 tcgaagatgt ccatgatctt gctgtcgcgc atccacttct ccgcgaggtg ctccttcgag
     7381 aatcccagcg gtcccaggag ctcgcagcac ttctgcgtga tgagcgtccc ctgccggccc
     7441 gctttcgcct tggccatcga cgcctcgacg ttgttgaact gctcggtgtc ggccatccag
     7501 gcggcgcgcc acaccagcag gcgcattgcc tccaggtgcg cctccatcag atagaaatcc
     7561 ttctcgagag aaccgacatt gttgagattt cgcttgtagt cgatggtcgc gccgctctcg
     7621 tcgagcttct cgcgggtgaa atcgagcgac gcgcgcgcga tccccgtcgc catcgacgcg
     7681 accatcggcc gggtattgtc gaaggtcttc atcacgccct tgaagccctc ggtgtccttc
     7741 ttcacctcgg ccgatcccag gatgttgtcg aatggaatac ggcactcgtc gagcacgaag
     7801 gtgccggtgt ccgatgcgcg gatgcccagc ttgtgctcga ggtgctcgag tttgaagccg
     7861 ggcgtgccct tttccacgat gaaggatttg atgccggact tcccggcgct cctgtcgacc
     7921 gtcgcccata cgacgacgat gtcgcaccgg tcggcacagg tcacgaagat cttctcgccg
     7981 ttgagcaccc actcgccgcc gtcgcgccgt gcggtggtgg tgatggagcc ggagtccgat
     8041 cccgagcccg gctcggtgat ggccatcgcg gcccacttgc cgccgaagcg ctcgagctgt
     8101 tcgtcggtcg cgacggcggc gatggcggcg tttcccagcc ccgcattggg gatcgagagg
     8161 aggagccccg cgtcgcccca gcacatctcc tcgatggtgg ccacggtcga aaggttgatc
     8221 cccttcttgg gctttccgtt tccgccgtcg ggcttcggcg catccgcctt tttcttcttg
     8281 gggcgcccca ggatctgcat gccgcggaac atgtccagct cggcgggata ctcgtgctcg
     8341 gcggcgtcgt acttgcgcgc gatgggccga aggacccccg cggcgagatt gttcatgctg
     8401 gaccgcatgc tggcaaggta gtcgggtgtt tcgagattta tcatgatggc ctcacatcct
     8461 catttgggat atattgttta ttgttcttgt gatattgctc agagcggcga cggtatcgca
     8521 acgaacaggc ggccaggatg gccgccggag cggtgccgtg cacggatgca catcaccgcg
     8581 gggtgagtgc gatatcgtcg ccgctctgag cgattaagaa attgcatggg tttcattgta
     8641 gtgttctgat ctttacgcga ttgccatgcc ctcgaggatg gcgattccgc ggccgttgcg
     8701 gtagtagcgc tccacgggat gctcacggat atagccgtgc ccgcccagga cctgcacgcc
     8761 gtagtcggtg agcttcatcg ccatctcgcc ggcgtagatg cgcgcgaggt acgcctcccg
     8821 ctgcgcgtcg cgcccggcct cgagggcgga cgccgccttc caggtcatca gccgcatagc
     8881 gtcaacctcg taggccatct cggcgatcat gaaggcgatc gactggcggc tcgcgatcgc
     8941 ctctccgaac tgctcgcgct ccttcgcgta attcttcgca tattcatacg atgcgcggca
     9001 gatcccggtc ccgatcgcgg cgatcgccgt ccaggtccgc tgcagcagcc ggtcgtagtc
     9061 gcagccggca tcgcccccca gccggtcatc cgctgggatc tcgcagtccg tcagggtgat
     9121 cgggtgtgtc gccaacgagt tcaggccaag gttcttctcg cgcgcgccga cggtcaggcc
     9181 gggattgttt ctgtcgacga tgaacaggcc gttcgtcccg tcgagcgccg ccgcgatcat
     9241 gatccgccgg gcggtctcgg ccagcggcac gaagcacttg gtcccgttca ggatatacga
     9301 tccgttcttc cgcacggcgg tcgtcgcgag gtcgtaggga tcgaaacgaa agcgcggctc
     9361 gttgattgcg agcgtgccgc atgcgccgtc cggatcggcg gcgaaatccg cgagatgccg
     9421 cgccttctgc gcgtccgtcc cgcagtccat cacgggcata aggaagagcg ccggcagggt
     9481 ggcggcgatc gcgaagccca tgtccccggc ggcgagctcc tcgaggatga tgctgttcat
     9541 gatgggagag ttccccatgc catagccgcc gtattcctcg ggaatggccg cgagcagcgt
     9601 ccccagctcg cggaattttc ggacggcgtc gtccgggatg cggcccgttt cgtccatctg
     9661 atgggccgcg tcggcgatag cgccctggac cagttttgcg gccgtttcct tcatgagggt
     9721 ttgttcttcg gaacatgcga agcagatcat tgcgtcctcc acggctgtaa ttgtactgac
     9781 atgtcagtct attcgtaccg ccgggaattg caagcacttt tttcctgcgg ggcgatgccg
     9841 tgggtcactt cgcgaagagg ccgggggaga tgaagccgac gatgcggtcg gtgatggcgt
     9901 cgatgtcgcg ggagtccttg tgcgacttgt gctggacgaa gtagtagtag gagacgcgga
     9961 agagcgcccc cacgatgatg ttcgcgaaga gctcgcggtc gatgtcggcg cggacggtct
    10021 tgtatttctg tccgatgacg aggtcgtcga tgatgacgcc ggcgatcttt ttctcgaagc
    10081 gcttgatgac ggcgtcgaag ctcttgtgta tcccgacccc catcctcagg acgatgttgc
    10141 acatctcatt gtcgccggcg aagaagagga gggtggtgcg tatgcgcgtc cggaggtagg
    10201 cctcaggcga gaccgcgtcg aggtccacat tctcggaggc cgacgcgatc aggcgctccc
    10261 aggcggtgta gaactcctcg agcagggtgg tgaagatgtc ttccttgtgc ttgaagtact
    10321 ggtagaccgt gccgcgcgcg atgtcggcct ccgtgatgat gtcggaaatc tgcgtctcgt
    10381 agaagccgtg tcgcgagaag agcttcttgg cgcagcggag gatgtgtttt tttcgttctt
    10441 cggcgttcat ggcgg
//
LOCUS       DAORHF010000125        10441 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5659_length_10441_cov_5.013191, whole genome shotgun sequence.
ACCESSION   DAORHF010000125 DAORHF010000000
VERSION     DAORHF010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5659_length_10441_cov_5.013191"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PKJ16_11140"
     CDS             <1..311
                     /locus_tag="PKJ16_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47585.1"
                     /translation="SSPLRPAALPRGPAADLITELYVAREKLSTRAWNKHSDGTNVPS
                     RTWDSYCTDIGIEKRTANRWLAQYFGGTVLATPSTGTRGHNIDCEIAARFFMRHNKKT
                     "
     gene            483..632
                     /locus_tag="PKJ16_11145"
     CDS             483..632
                     /locus_tag="PKJ16_11145"
                     /inference="COORDINATES: protein motif:HMM:NF016136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HNV47586.1"
                     /translation="MLKDDFVMIRLPKETKRQFQDVMAQRGVNPADALRKFITDVIRK
                     NGGTA"
     gene            629..1348
                     /locus_tag="PKJ16_11150"
     CDS             629..1348
                     /locus_tag="PKJ16_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47587.1"
                     /translation="MSEKETRIDVESGHTARFMELSRHFSEIHKRAEKLEQEGFTLQN
                     KRDALQVKLKAAIIDDAINNTNKSEALTMELYKLDAKIKITGEQHKLLARRRDDADFQ
                     AMMRSSAGVSDIARSVINENKAAIAELRARYETEKAELTTMKIAYLEKVHNIGRLVDY
                     GEMLVEESRDALPYSGLAVQVDDFVASDIDHRHWRGSIYDGITPEVITETFKGPAAKS
                     FRKTVGRDLPLLHLSSIQQGE"
     gene            1353..1649
                     /locus_tag="PKJ16_11155"
     CDS             1353..1649
                     /locus_tag="PKJ16_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47588.1"
                     /translation="MEDLKELIRQRDAINGAIAKNMLEAQGGTVPVDHDMFRDEVRKN
                     LSLKTMAFVGEAAKLKERIATIAAIPKAQLDYPTMVERHRAEKRLAEIEREATV"
     gene            1646..1771
                     /locus_tag="PKJ16_11160"
     CDS             1646..1771
                     /locus_tag="PKJ16_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47589.1"
                     /translation="MNTIIETVRAYFADLALDRDIARLERRIRRLEDELRARGNL"
     gene            1805..2107
                     /locus_tag="PKJ16_11165"
     CDS             1805..2107
                     /locus_tag="PKJ16_11165"
                     /inference="COORDINATES: protein motif:HMM:NF039194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HNV47590.1"
                     /translation="MAKSSSSWKKGQSGNPKGRPRTGFSMAELLYRRLDTKHGGRTKK
                     QVVVDNLIALAIEKDLRAIRILFEVLLRTYEFDKRNEIEERIEALEKTMEGQCQKG"
     gene            2092..2280
                     /locus_tag="PKJ16_11170"
     CDS             2092..2280
                     /locus_tag="PKJ16_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47591.1"
                     /translation="MSKRLKDRLTRLENRAGVAVEMIEFVINRHFMPDIGPMPHRRDR
                     QGRYHIMCPRVLSHHGTK"
     gene            2302..2634
                     /locus_tag="PKJ16_11175"
     CDS             2302..2634
                     /locus_tag="PKJ16_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47592.1"
                     /translation="MTNTITLADRKTRKEYPVDIFVPATLAVLLAERLDILRKALAGT
                     VDDSVLELVFQVEEMIFKPQYDFMTAAWCEKNVDLATTLYLIWRISKPLLEYLDAVGV
                     FPFKPVEV"
     gene            2857..5184
                     /locus_tag="PKJ16_11180"
     CDS             2857..5184
                     /locus_tag="PKJ16_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47593.1"
                     /translation="MKAASNLQETQQKFGVVFQNNIKQAEQFAKTLQEGYGLSENAAK
                     AMLSHTGDILQGFGMQEKAALDLSMEVQKLTVDLVSFTNYQGTVEDASRAITKAMLGE
                     AEALESLGKKILDDDVKRRAQADGYKLVNGQIDNQARAVTVLKLLTEQSKNAIGDFQR
                     SSDSFANTMRVVDARLEDISAEIGKELLPSLSTLFSQILDGDKATNPFIQGLLGIARA
                     AATAVDGITALAAKYSMTINEVTINKQREGLELLNKAEKELLDNAEKQYAAQGKVFKN
                     RSELLSSLKQEYEQQAATGDAAHHTYEVYEKITTTKRALAEETSKLADANVKNEERLE
                     KVVKRINGIEEERAASANNAAKQEIRTSRVRTQLTEEQIAKLREMYREWLEQYLANGD
                     VELASIETRYEKELQKYKDMLRAKVMSYGEYVMAVAELEEKREKDKEREAIKKEIKEY
                     EESQKFIADIRSALDLKTELSQKLLTIRSDFNKAMLESTNEDLVGRYGAQVKAIAEAG
                     FLGSLSVAASYYAGMNELQMMAAQNRLTAIDYETQTELESIASRYESEKATIEATVKD
                     RGERDAALKALDEKRSRDERAVQERADKEKRKIQHETAKQQKIINKTETIIAGAVAAI
                     EAYKAMSGIPVVGPALGAAAASAITALTYEKVRLIEEQPLPPLARGALIQRTQGGTPV
                     IVGEGRHDEAVIPLNAATFDMLGERMVAALMSRAVNHSVTNTVTNIDNRATVEHPREP
                     IHIHNVIELDGEVISEFVTKATDDARILINPRAIK"
     gene            5181..5408
                     /locus_tag="PKJ16_11185"
     CDS             5181..5408
                     /locus_tag="PKJ16_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47594.1"
                     /translation="MTDAPVNDKTAGLTQREIDFVNDLVDRFERGGIASLAREDVVAF
                     AKIIDHVQGAPDDVKGFFALAEKELTGCTEQ"
     gene            complement(6005..6187)
                     /locus_tag="PKJ16_11190"
     CDS             complement(6005..6187)
                     /locus_tag="PKJ16_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HNV47595.1"
                     /translation="MAAKFEVYKDGKGEYRFRLKAANGEIIAVGEGYSNKQGCLNGIE
                     SIKKNAPIAEIVEVEK"
     gene            complement(6383..8350)
                     /locus_tag="PKJ16_11195"
     CDS             complement(6383..8350)
                     /locus_tag="PKJ16_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47596.1"
                     /translation="MIEISNISKTYRMGEVEVRALRNVSLSIGEGEFVAIMGASGSGK
                     STLLHVIGFLDKPDSGTYALYGHDITRLSDDELSMLRNRTAGFVFQHFYLLPRMTALE
                     NAMLPLVYAGKRRDEHSARSRIEAVGLAARSGHYPNEMSGGEQQRVAIARALVNEPLI
                     IFADEPTGNLDTKNEEEVLAVLRRLNDDGKTVVMVTHENEVAAQAKRIIRMRDGEIVS
                     DERRGKPRGSSPRKSAEGPPRKNIFATASAALDRAEFADYIRQAAGSILAHKLRSFLS
                     VLGILIGVAAVISMLALGEGAKASVSESLSALGTNLLTVRPGSHRMRHVALETGSVTR
                     LTVQDAHALSMISGVARTSPSVMGRAQLVYGNRNWNSQAQGVGTAYAAMKSMQPVAGR
                     FFTEDELRNRSKVAVLGSTVARELFKGASPLGRTIKINRINFHVIGVLPVKGVSFFRD
                     QDDVVLLPVTTAMFRLLGREFVDFIEVEVASADQIERAMGAIAGLIKRRHRIAAEDDE
                     AFHIRDMSEMRAAMTGTIDTISILLGIVASISLFVGGIGIMNIMLVSVKERIKEIGLR
                     KAIGARKRDIRTQFLIESAMMTFAGGVMGILIGSGLSLLLSLFAGWSIMISPFSIIAS
                     TLFSVLIGVFFGLWPALQAARLNPIEALRYE"
     gene            complement(8357..9316)
                     /locus_tag="PKJ16_11200"
     CDS             complement(8357..9316)
                     /locus_tag="PKJ16_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011361382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV47597.1"
                     /translation="MIGKRAIAIAVVVVAVITGAFFIYRGRQGGGGDDSRQRIAPEYG
                     SIRSVISTSGTVQPRNRLEIKPPVNGRIESILVVEGRLVRKGQTLALMSSTERAAFID
                     VARTQDAATLKYWEDAYKPIPLIAPIGGTVIVRAVEPGQTVTTATAVLVLSDRLIVKA
                     EIDETDIGRVRLGQSAAIQLDAHPDIRAQGRVSHISFESKTINNVTMYEVEVVPEKIS
                     AEFRSGMSANISIIERVRDRALLLPLEAVTIDENGSSVLVATEGTKPQRRAVTTGLSD
                     DRNVEIVSGLSIDDRVALPEKKRAAKQAPARGGTPFLPSRPRR"
     gene            complement(9306..>10441)
                     /locus_tag="PKJ16_11205"
     CDS             complement(9306..>10441)
                     /locus_tag="PKJ16_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV47598.1"
                     /translation="RCIHCSVGMTRSYNEYNETDAESSSQSSSVFGSGVSNSGTTADS
                     YSYGFTARQLVFDGLKSVYDIRYSRAKVAEAEYQYRGVSASVRLDLRTAFVQLLKAQD
                     LIVITEEIVKLRKKNSDLVHMRYKAGREHRGAYLVARANLSQAEFEHAQALRNVIIAR
                     RQLVKEMGLQTYYPIAARGELEPAPYGKTPDFVELVRANPLMRKAITQKESARFNRDA
                     ALANLSPRIDLFGGANRSGSRWAPQNYGWHIGIEATYPLFEGGKSYYQAKKAQAELKQ
                     VIADALGTQEKLLVQLELRWNALMNETDRIKVQGDFLKASEERSRIAEMQYNVGFVQF
                     DNWIIIVDDFVRAKKNLLEAKTSALLAEAEWMYARGEMLSYDR"
BASE COUNT         2297 a         3177 c         3056 g         1911 t
ORIGIN      
        1 gatcttcgcc tctgcgtcca gcagcgctcc cgcgaggtcc tgcagcggat ctcatcaccg
       61 agctgtatgt ggcgcgggaa aagctgtcaa caagagcatg gaataaacat agcgatggga
      121 caaatgtccc atcgcgtacc tgggacagtt attgcaccga cattggcata gaaaagcgca
      181 cggccaaccg ctggctggca cagtatttcg gcgggaccgt gcttgccacg ccgagcacag
      241 gcacacgcgg acataacatc gattgtgaga ttgcagcgcg ttttttcatg cgacataata
      301 aaaaaacttg acaaatacac gcatgtttag cttactattg attagtgact acgttttcac
      361 gccaaccata attgattaac accttctgac tctgcgcgcg ccgcaacacg gggagcaccg
      421 gcacccgggg caccacgagc cggccgccag cgtccgggca tcacaacaaa gagaggtcaa
      481 tcatgctgaa agacgacttc gtaatgatac gccttcccaa ggaaacgaag cggcaattcc
      541 aagacgtcat ggcgcagcgc ggcgtcaacc cggcggacgc gctgcggaaa ttcatcaccg
      601 acgtcatacg caaaaacggg ggcacggcat gagcgaaaag gaaaccagaa tcgacgtgga
      661 aagcggacac accgcgcgct tcatggagtt gtcccggcat ttttccgaga tccacaagcg
      721 cgcggaaaag ctcgaacagg agggcttcac gcttcaaaac aagcgggacg cactccaagt
      781 aaagctgaag gccgccatca tcgacgacgc gatcaacaac acgaacaaat cagaagcgct
      841 gacgatggag ctctacaagc tcgacgcaaa gatcaagatc accggcgagc aacacaagtt
      901 actcgcgcgc cgtcgcgacg acgcagactt tcaagcgatg atgcggtcct ccgcgggggt
      961 ttctgacatc gcgcgctcag tcatcaatga aaacaaggcg gccattgctg agcttcgcgc
     1021 acggtacgaa accgaaaagg ccgagctaac cacgatgaaa atcgcctatc tcgaaaaagt
     1081 ccataatatc ggccggcttg tcgattacgg cgaaatgctt gttgaagaat cgcgcgacgc
     1141 gctcccgtat tccgggcttg ccgtccaggt tgacgacttc gtggcaagcg acatcgacca
     1201 ccgccactgg cgaggatcaa tttacgatgg aattaccccc gaagtgataa ccgaaacatt
     1261 caaaggtcct gccgcgaaat cgttcagaaa aaccgtgggc cgcgacttgc cgctactgca
     1321 cctgagttca attcaacaag gagagtgaca tcatggaaga ccttaaggaa ttaattcgac
     1381 agcgcgacgc cattaacggc gccatcgcca aaaacatgct cgaggcccag ggcggcaccg
     1441 ttcccgtgga ccatgacatg tttcgcgacg aggtgcgaaa aaatctgtcc ttgaagacga
     1501 tggcgtttgt cggcgaggcg gcgaagctta aagagcgcat cgccaccatc gccgccatcc
     1561 cgaaggccca gctcgactat cccactatgg tggagcgtca ccgggccgaa aagcgactcg
     1621 ccgagatcga gcgcgaggcg acagtttgaa cacgatcatc gagacggtcc gagcgtattt
     1681 cgccgacctc gccctcgacc gtgacatcgc gcggctcgag cggcgcatcc ggcggctcga
     1741 ggatgaactg cgcgcccgcg ggaaccttta acagcggggc gctgctaaca cggtaaaaag
     1801 cagagtggcg aaatcttcat cttcctggaa aaagggacaa agcggaaacc cgaaagggcg
     1861 accgcgcacc ggcttttcga tggccgagct cctttaccgg cgactggaca ccaagcacgg
     1921 cggccgaacg aaaaagcagg tcgtcgtcga caacctcatc gcgctcgcaa tcgagaagga
     1981 cctgcgcgcg attcggattc tcttcgaggt ccttctcagg acatacgagt ttgacaagcg
     2041 gaacgagatc gaggagcgca tcgaggccct cgagaagacg atggaggggc aatgtcaaaa
     2101 aggttgaagg accggctcac gcggctcgag aaccgggcgg gtgtcgccgt cgagatgatc
     2161 gagtttgtca taaacaggca cttcatgccg gacatcgggc ccatgccgca ccggcgcgac
     2221 cgccaggggc ggtatcacat catgtgtccg cgggtgcttt cgcaccatgg cacaaaataa
     2281 cagttgtgag gtaataacga cgtgacgaac accatcaccc tcgccgaccg aaagacccgg
     2341 aaggaatacc cggttgacat cttcgtgccg gccacgctgg cggtccttct tgccgaacgg
     2401 ctcgacatcc tgcgaaaggc gctggccggc accgttgacg actccgttct cgagctggtt
     2461 ttccaggtcg aggaaatgat tttcaagccg cagtatgact ttatgacggc cgcctggtgc
     2521 gagaagaacg tggacctcgc cacaacgctt tacctgatat ggcggatatc gaagccgctt
     2581 ctcgaatacc tggacgccgt cggcgttttc ccgttcaagc cggtagaggt gtaaagcatg
     2641 ggtgaaataa acgtcggcca ggtgtattat gacgtaacgg cgaggctaga ccagctcgag
     2701 aaggggctcg cccaggcgcg cgacaaactg acggccgtcg gcaagaacgc caagggcgcg
     2761 tcggaatcaa tcaccgccgg tttcctgaaa agtcaaatcg tcatcgaggc ggccacgcgc
     2821 gcgtttcgtt tcctctccga caccatgcag aaatccgtga aggcggcgtc gaaccttcaa
     2881 gagacgcaac agaaattcgg cgtcgtcttc cagaataaca tcaagcaggc cgagcagttc
     2941 gcgaagacgc tgcaggaggg ctacgggctt tccgagaacg ccgcgaaggc catgctctcg
     3001 cacaccggcg acatcctgca gggcttcggt atgcaggaga aggcggcgct cgacctctcg
     3061 atggaggtcc agaaactcac cgtcgacctg gtgagcttca cgaattacca gggaaccgta
     3121 gaggacgcga gccgggccat cacgaaggcc atgctgggcg aggccgaggc gctggaatcg
     3181 ctcggaaaga agatcctcga cgacgacgtg aagcgccggg cgcaggccga cgggtacaag
     3241 ctcgtcaatg gtcagattga caaccaggcg cgggccgtaa cggtgctgaa gctcctcacc
     3301 gagcaatcaa agaacgccat cggagacttc cagcgcagct cggactcgtt cgcgaacacc
     3361 atgcgcgtgg tcgacgcgcg tctcgaagac atctcggccg agatcggaaa ggagctttta
     3421 ccgtcccttt ccaccctttt ctctcagatt ctcgacggcg acaaggcgac caatccattt
     3481 attcaagggc tgctcggtat cgcccgggcc gcggctaccg ccgttgacgg catcaccgcc
     3541 ctggccgcca agtactcaat gaccataaac gaggtcacga tcaacaaaca gcgcgagggc
     3601 ctcgagcttc tcaataaggc cgaaaaggag ctgctcgaca acgcggaaaa gcagtacgcc
     3661 gcccagggga aggtattcaa gaaccggtcc gagcttctca gttcgctcaa gcaggaatat
     3721 gaacagcagg cggcaaccgg ggacgccgcg caccacacct acgaggttta cgagaagatc
     3781 acgaccacca agcgcgcgct ggcggaagaa acttcaaagc tggccgacgc aaatgtcaaa
     3841 aacgaagaac gcctcgaaaa ggtggtcaaa cggattaacg gaattgagga agagcgggcc
     3901 gcatccgcga acaacgcggc aaagcaggaa atccgcacaa gccgggtacg aacacagctc
     3961 accgaagaac agatcgcgaa gctgcgagaa atgtatcgcg aatggttgga gcagtacctt
     4021 gcgaacggcg atgtagagct cgcgagcata gaaacgcggt acgaaaaaga gctccaaaag
     4081 tacaaggaca tgctgcgcgc caaggttatg agctatggcg agtatgtgat ggccgttgcc
     4141 gaactcgaag aaaagcgaga gaaggacaaa gaaagggaag ccatcaagaa agaaattaaa
     4201 gaatacgaag aatcacaaaa atttatcgcg gatattcgtt ctgctctgga cctcaaaaca
     4261 gaattaagcc aaaagctttt aacaattaga agtgacttca acaaggcaat gcttgaatcc
     4321 accaacgaag acctggttgg tcgatatggc gcccaagtga aggcgatagc cgaagctggt
     4381 tttttgggga gtttgtcggt tgcggcatct tactatgccg gcatgaatga attacagatg
     4441 atggcggcac aaaacagatt aaccgcgatc gactacgaga cacaaaccga gctcgaatcc
     4501 atcgcctcgc gatatgaatc cgaaaaggca accatcgagg cgacggtcaa agatcgcggt
     4561 gagcgcgacg cggccctgaa ggccctcgac gagaagcgct cccgcgatga gcgcgccgtt
     4621 caggagcgcg ccgataaaga gaaacgcaag atccaacacg aaacggccaa acagcagaaa
     4681 atcattaata aaaccgaaac gatcatcgcc ggcgccgtgg cggctattga agcctacaag
     4741 gccatgtcgg gaatacccgt cgtgggcccg gcgctcggtg ccgcggcggc ctcggcgatc
     4801 accgccctga catacgagaa agtccgcctg attgaagaac agccgctccc accactggcc
     4861 cgcggcgctc tcatccagcg gacccagggc ggaacgccgg ttatcgtcgg cgagggccgc
     4921 cacgacgagg ccgtgatccc gctcaatgcc gcgaccttcg acatgctggg cgagcgcatg
     4981 gtggccgctc tcatgtcccg agcggtgaac cactccgtca cgaacaccgt gacgaacatc
     5041 gacaaccggg cgacggtgga gcacccgcgc gagccgatac acatccacaa cgtgatcgag
     5101 ctcgacggtg aggtgatatc cgagtttgtg acgaaggcaa cggacgacgc gcggatcctg
     5161 attaacccga gggcgataaa atgacagacg cgccggtaaa tgataagacc gcggggctca
     5221 cccagcggga aattgacttt gtaaacgacc tcgtcgatcg cttcgaacgt ggcggcatcg
     5281 cgtccctggc gcgcgaggac gtcgtcgcct tcgcaaaaat catcgaccac gtccagggtg
     5341 cgccggacga cgtgaagggc tttttcgctc tcgcggaaaa agagctcacc ggctgcaccg
     5401 agcagtaaca gcgtcgtaat atcccttgtg tggtaaatcg cccaaaaact gtttgacata
     5461 aagccggctt tctgctttcg ttttcatcat gaaagcggat cgcggcatcg ttgaacatcg
     5521 cgtttgtgtg tacgataacc ccgacgttat catgattgac gccgccgcga tgacgatcca
     5581 gtttgccgac cgcgtcgcct ttcgtgagac ggtccacgcc ctcgcaaaaa ccaaccaggt
     5641 cttcgcgacc gcatcgtaaa ctcccccatc cgagtgtagc ataactgtag cacttttccg
     5701 cgctcacctt aacgcatgtt taccgtgctt tgtgtttttt gcttgacgtt tccgctcacc
     5761 atgattatgt ttacagcacg atagcgcctg ttagtgccat aagtaccgtg ctcgtaatga
     5821 gcaggtcacc agttcaactc tggttgctgg ctacgcggac agaaacgacg ccccggacgg
     5881 ttaaaccgtt cggggcgtta tcatttgcga ctcacgtggc cgcgtatagg cgacattcca
     5941 tgcgcctttt tcgatatgcg acattcccgc aaaccgggtg gcgtcgcacc aaataaaatc
     6001 cggcttattt ctccacttcg acaatctctg caataggggc gtttttcttg atcgactcga
     6061 tcccgtttaa acaaccctgc ttgttcgaat agccctcgcc caccgcgatg atttcaccat
     6121 tcgccgcttt cagccgaaac cggtactccc cttttccgtc cttgtacacc tcgaattttg
     6181 ccgccatcgc atcctccttg tcgttatatt cacaatacag tatacttaaa tttttccttc
     6241 aattcaagcc tttttcatga aaagtgacga tgcggcttcg agcgcccacc tgttccctgt
     6301 atgattcggc ttcgccgtta tcgtgtcgat gaaatgttcg ctgatgagtc cggccgcacc
     6361 ggcatcgacc gaagtctccc gttcactcgt agcgcagcgc ctcgatggga ttgagccgcg
     6421 cggcctggag cgccggccaa agcccgaaga acacgccgat gagcacggaa aagagcgtcg
     6481 aggcgatgat tgagaacggc gaaatcatga tgctccatcc ggcgaacagg gacaacagca
     6541 gggagagtcc gctgccgatc aggatgccca tcaccccccc ggcgaaggtc atcatggccg
     6601 attcgatgag gaactgcgtg cggatgtcgc gtttccgcgc gccgatcgcc ttgcgcagcc
     6661 cgatctcctt gatgcgctcc ttcacggaca ccagcatgat gttcatgatg ccgatcccgc
     6721 ccacgaacag cgagatcgag gcgacgatcc ccagcagtat gctgatggtg tcgatggttc
     6781 ccgtcatcgc ggcgcgcatc tcggacatgt cgcggatatg gaacgcctca tcgtcctcgg
     6841 cggcgatccg gtgccgccgc ttgatgagcc ctgcgatggc gcccatggct cgctctattt
     6901 gatccgcgct ggccacctcc acctcgatga aatcgacgaa ctcgcgtccc aggagccgga
     6961 acatcgccgt ggtcacggga aggagcacca cgtcgtcctg gtcgcggaag aacgagacac
     7021 ccttgacggg aaggaccccg atcacatgaa agttgatgcg gttgatttta atcgtacgcc
     7081 ccagcgggct tgcgcctttg aagagttcgc gggccacggt gctgccgagc acggcgacct
     7141 tgcttcgatt gcgaagctcg tcctcggtga aaaatcgccc cgcgaccggc tgcatcgatt
     7201 tcatcgccgc gtacgcggtc cccaccccct gcgcctggct gttccagttg cggttgccgt
     7261 acaccagctg ggcgcgtccc atgaccgacg gagacgttcg agccacgccg gaaatcatcg
     7321 aaagcgcgtg cgcgtcctgg acggtgaggc gcgtgaccga accggtctcg agggccacgt
     7381 ggcgcatccg gtgggacccg ggccgtacgg tgaggaggtt ggtgcccagc gcgctcaggc
     7441 tttcggagac ggaggccttc gccccctcgc ccagcgccag catggagatg acggcggcca
     7501 ccccgatgag tatccccagg acggagagga aggaccggag cttgtgcgcc aggatggagc
     7561 ccgccgcctg ccggatataa tcggcgaact cggcgcggtc gagcgcggcc gacgcggtcg
     7621 cgaagatgtt tttccgcgga gggccctcgg cggattttcg gggagacgag cctcgcggtt
     7681 tgcctcggcg ctcatcggac acaatctcgc cgtcgcgcat gcggatgatc cgcttcgcct
     7741 gcgcggccac ctcgttttcg tgggtaacca tcaccacggt ttttccatcg tcattgaggc
     7801 ggcgcaggac ggcgaggacc tcctcctcgt tcttcgtgtc caggttgccg gtgggctcgt
     7861 cggcgaagat gatgagcggc tcgttcacca gcgcgcgggc gatggccacg cgctgctgct
     7921 cgccgccgga catctcgttg ggatagtggc cgctccgggc ggcgagtccc acggcctcga
     7981 tgcgggagcg cgcgctgtgc tcgtcgcgcc gcttgccggc atagacgagc gggagcatgg
     8041 cgttctcgag cgccgtcatg cggggcagca gatagaagtg ctggaacacg aacccggcgg
     8101 tgcggttgcg cagcatggaa agctcgtcgt cggagaggcg ggtgatgtcg tggccgtaga
     8161 gcgcgtaggt tccgctgtcg ggcttgtcca gaaaaccgat cacgtgcagg agcgtcgatt
     8221 ttcccgatcc cgacgccccc atgatcgcga cgaactcccc ctcgccgatc gagaggctca
     8281 cgttccggag cgcccggacc tcgacctctc ccattcgata ggtcttggat atgttggaaa
     8341 tctcgatcat ctgcgcttac cggcgcgggc gcgagggcag aaacggggtg ccgccgcgtg
     8401 cgggcgcctg cttcgccgct cgttttttct ccgggagcgc gacgcggtca tcgatggaaa
     8461 gcccggagac gatctcgaca ttgcgatcgt cgctcaaccc ggtcgtcacg gcgcgccgct
     8521 gcggctttgt gccctccgtt gcgacaagga ccgagcttcc attctcgtca atcgtcacgg
     8581 cctccagggg aagcagcagg gcccgatcgc gcacgcgctc gatgatgctg atgttggcgc
     8641 tcatccccga gcggaactcc gcggatatct tctccgggac cacctccacc tcgtacatcg
     8701 tcacgttgtt gattgtcttc gactcgaagg agatgtggct caccctgccc tgcgcgcgaa
     8761 tgtcgggatg cgcgtcgagc tggatcgcgg cgctttggcc aagccgcact cggccgatat
     8821 cggtctcgtc gatctcggcc ttcacgatga ggcggtcgga gagcaccagg accgcagtag
     8881 cggtggtcac ggtctgtccc ggctccacgg cgcgcacgat caccgtcccg ccgattggcg
     8941 cgatgagcgg aatgggcttg taggcgtcct cccagtactt gagggtcgcg gcgtcctgcg
     9001 ttcgcgcgac gtcgatgaag gccgcccgct ccgtcgagct catcagcgcg agggtctggc
     9061 ccttgcgcac cagccgtccc tccacgacca ggatcgactc gatgcggccg ttcaccggcg
     9121 gcttgatctc cagccgattg cgcggctgca ccgtccccga ggtggagatg accgagcgaa
     9181 tgctcccata ctcgggcgcg attcgctgtc gggaatcatc gcccccgccg ccctgccgtc
     9241 cccgataaat gaaaaacgcc cccgtgataa cggcgacgac caccacggcg atcgcgattg
     9301 cgcgcttacc gatcatagct cagcatctcc ccgcgcgcat acatccactc cgcctccgcc
     9361 aggagcgcgc tcgtcttcgc ctcgaggagg ttttttttgg cgcggacgaa atcgtccacg
     9421 atgatgatcc agttgtcgaa ctgcacgaac ccgacattgt actgcatctc ggcgatccgc
     9481 gaccgctcct cggacgcctt cagaaaatcg ccctgcacct tgatgcggtc cgtctcgttc
     9541 atgagcgcgt tccagcgcag ctcgagctgg accaggagct tctcctgcgt gccgagggcg
     9601 tccgcgatca cctgcttgag ctcggcctgc gcctttttcg cctgataata gctcttgccg
     9661 ccctcaaaga gcggataggt cgcctcgatg ccgatgtgcc agccatagtt ctggggcgcc
     9721 cagcgactgc cgctcctgtt cgccccgccg aacaggtcga tccgcggcga gaggttcgcg
     9781 agcgcggcgt cgcggttaaa gcgggccgat tccttttggg tgatggcctt gcgcatcagc
     9841 ggattggccc gcaccagctc aacgaagtcc ggcgtcttcc cgtacggcgc cggctcgagc
     9901 tcgccccgcg ccgcaatggg atagtaggtc tgcaggccca tctccttgac gagttggcgc
     9961 cgcgcgatga tgacattgcg aagcgcctgc gcgtgctcga actccgcctg cgacagattc
    10021 gcccgggcga ccagataggc gccgcggtgc tcccggccgg ccttgtagcg catgtgcacc
    10081 aggtccgagt tcttcttcct gagcttcacg atctcctcgg tgatgacgat gaggtcctgc
    10141 gccttgagga gctgcacgaa ggccgtccgc aggtccaggc ggacgctcgc cgacacgccg
    10201 cggtactggt attccgcctc cgccaccttc gcgcggctgt acctgatgtc gtagacgctc
    10261 ttgagaccgt cgaagacgag ctgccgggcc gtaaacccat acgagtagct gtcggcggtc
    10321 gtcccggaat tggaaacgcc gctgccgaaa acggacgacg actgggatga cgactcagcg
    10381 tccgtctcgt tgtattcatt gtagctgcgg gtcatcccca cgctacaatg aatacaacga
    10441 g
//
LOCUS       DAORHF010000126        10316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5767_length_10316_cov_4.879641, whole genome shotgun sequence.
ACCESSION   DAORHF010000126 DAORHF010000000
VERSION     DAORHF010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10316
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5767_length_10316_cov_4.879641"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PKJ16_11210"
     CDS             <1..832
                     /locus_tag="PKJ16_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47599.1"
                     /translation="ISSGSESVSDSMEEQSAAMESLARLIGELTAITGTMEARITDSL
                     AKTKEIARRAQAGETHIMGMSRSMEEINDTARQMTGILNIINDISDRINLLSLNASIE
                     AARAGEHGRGFAVVADEIGKLADQTSTSVKEIDALIKKSEQEIGRGLVTVQDTVETMR
                     GIIEGVNVSNNTISDINASMDQYVKSNDSVRSETDRAKQRSDEIKTAMLEQKKAAEEI
                     VGTITGVNNISQENAISAEEIKRQAEMIADMALDLRDKISKFDEEWVDAPAERFGPEA
                     "
     gene            1002..1325
                     /locus_tag="PKJ16_11215"
     CDS             1002..1325
                     /locus_tag="PKJ16_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HNV47600.1"
                     /translation="MPTNTNSDHDLDGDLALDSRDEVEHPKMYRVIMHNDHYTTMDFV
                     VEVIIAVFHKPAAEATRMMLEIHKQGMSVCGVYTYDIAATKTGQVHQMARERGFPLKC
                     SFEEA"
     gene            1339..3600
                     /gene="clpA"
                     /locus_tag="PKJ16_11220"
     CDS             1339..3600
                     /gene="clpA"
                     /locus_tag="PKJ16_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HNV47601.1"
                     /translation="MDISDELNQILTAAYNEAKNRKHEYLTPEHVLYASLFFSGAKEI
                     IENCGGNVDRLKTSLEKHFEKMITPLPDGADPAQSVGFQNVLERAIFHITSAQKDLLD
                     IGDVYVSIFDEQESFAAYFLKREGITRLDLLNYVSHGISVVPSGASAGDAAPEIDDED
                     EAAAGDKKKDRKALQLFTAELTQKAKQGELEPLIGREDILERTLQVLCRRFKNNPIHV
                     GDPGVGKTAITEGIAQLVADGRVPKPLKSAKIFSLDMGALLAGTKYRGDFEERLKRVL
                     QELGEQKNVILFIDEIHTVVGAGAVSGGSMDASNILKPALASGKIRCIGSTTYEEYRK
                     FFDKDRALSRRFQKIEIPEPTIDETHRILQGLREKYETYHEVTYTDEALRAAAELSAK
                     FINDRHLPDKAIDVIDEAGAYARMNRVEDEAKNEITAHDIERVVAKIARIPEKSVSAP
                     ETVRLKDLERELASQIFGQDKAVAAVAEAIKRSRAGFHETDKPVASFLFVGPTGVGKT
                     ELARQLARILGIAIHRFDMSEYQEKHTVARLIGAPPGYVGYEEGGLLTEAIRKTPHAV
                     LLLDEIEKAHTDIFNTLLQVLDYATLTDNNGKKADFRNVIIIMTSNAGAREIGRKKVG
                     FDERVVKDDAITEAIERFFSPEFRNRLDAIVNFNNLDEEIAVQIVRKELAEFQRQLDE
                     KKIALTVTDECCRWLAKRGFSPVFGAREIARVVREKIKKRFVDEVLFGRLANGGAVRI
                     DVKDDEAVFDIAS"
     gene            3606..4307
                     /gene="aat"
                     /locus_tag="PKJ16_11225"
     CDS             3606..4307
                     /gene="aat"
                     /locus_tag="PKJ16_11225"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HNV47602.1"
                     /translation="MPVYQLSEAIAFPHPSLATDEGVLAVGGDLSPERLLLAYANGIF
                     PWYSHGDPIIWWSPDPRFVLFPGELLVSKTMRQVLRRNVFTITFDRAFRAVIASCKKI
                     RRRRERGTWITGEMLEAYVRLHELGYAHSAEAWRDDELVGGLYGVSLGRCFFGESMFS
                     RESNASKAALITLTRRLAARGFGLVDCQVHTDHLESLGAREIPRDEFLVTLRGLVGAD
                     TLRGDWGALMPVEDR"
     gene            4446..5846
                     /locus_tag="PKJ16_11230"
     CDS             4446..5846
                     /locus_tag="PKJ16_11230"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HNV47603.1"
                     /translation="MIKDNAAEKIKHIKEMGRSVSIFDQKIFGVIVGVAALIVVALVV
                     YFVDFSPSLSHLRVTVFSGATTGNYHATVTTAADIARRRGGRIENIATNGSIDNIKRL
                     QQTKKRGQFALAQNGMPWGEGIELVAHLRAPETVFFLGPNADSIKTLADLKHKRIGIG
                     PKGSGTAHLAETIFNTPQFAGLAVSLSHHSSDEQLALLKQRTIDLGVFVISEKSVFIE
                     KAVCEDGMQIASFRQSESIAMRLPFLKSDYIREGLYDPVRNLPASSKKVLKLDTLIVS
                     NGEAKRSQVIGLLSVMSDIYPNLINYNRTVTNYTGLPESSAARDFFNNTGPEILDRYS
                     PRLMDILPLSNLVQLAMAISIFFNLMGVGNRFFLWRIDANRIALEVEMRDFFGRGILP
                     EEIEHMAAMEEHRTDDRRRSLTALIERLETLEGRCRKQSQGLLVPMGGEMAYRYQEEI
                     ISRNLVGLRAYRDKIA"
     gene            5998..6414
                     /locus_tag="PKJ16_11235"
     CDS             5998..6414
                     /locus_tag="PKJ16_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014405997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HNV47604.1"
                     /translation="MATYSSTIIFPTKGNRDIIDITDKIQAAINESGALNGVVVAFIP
                     GSTAGLTTIEYEPGLKKDMNLFLERILPHGDRYHHHDTWHDDNGAAHLQAAMIGPSIT
                     IPVVNGKMTLGTWQQVVCIDCDTRPRQREIVLQIVS"
     gene            complement(7221..7721)
                     /locus_tag="PKJ16_11240"
     CDS             complement(7221..7721)
                     /locus_tag="PKJ16_11240"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNV47605.1"
                     /translation="MMGKTVRTWVFRWALCAVVLGLAGAAGGVEKGVRAPLVPKVGDN
                     LRWGFVDHNDSFVINPRFEFAGPFHDGRALIRVSGKYGFIDPKGRLAIRTQFDDARNF
                     SEGLAAVMIMYGGSDRKWGYIDRAGSFVIPARFEYASDFLNGTAVVLENGKEVVVRAP
                     AHGKAP"
     gene            complement(7727..8338)
                     /locus_tag="PKJ16_11245"
     CDS             complement(7727..8338)
                     /locus_tag="PKJ16_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47606.1"
                     /translation="MNYTIVKQFWNNSVDNFYNFFYKIVDFFKIWIDVWWAFFEIWQC
                     FFLIFFNFFMYFYYLFLFMLDRSATESQSRIIFWKKIPQRVAYTPAKAYVKDVVNPIP
                     GMYGAQPVKKVAETVTATTAKVADVSQILRKAPSGGKVSIIRRALEGVSELFRSIKDF
                     FAGLFKPAAEMVGRKMKHVDEEPQSKSLIDEYMREYERKKKRA"
     gene            complement(8460..9869)
                     /locus_tag="PKJ16_11250"
     CDS             complement(8460..9869)
                     /locus_tag="PKJ16_11250"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HNV47607.1"
                     /translation="MNKPEQMKKRAFRKDAYIYIEGDEDVNDIYIVERGTIELVSEYE
                     KIKRFRSTVEVGGVFGFISALCRRPRMETAVAAEDVQVVVCPRAKFFELIQKNPDIAF
                     KIIRGFAEELRVYDEMMLRLIDQEPDTNTTETKFFNLGEYYFRKDQYPYAYYVLKRYA
                     GQYPGAFNRPAAMEMIAEIEKTGHRGIIEPMREGIYRVFSDRQMIFCEYEPGEELYII
                     KEGRVKIVKVSRENEVMLSVLKEGDIFGELAIVSNKPRNASAISWGIAKALPINRNTL
                     KTVLAKSPTIIHKIFMAISQRIWFTYIRLESKLYKKHITQIYAFLENQLLELNVSLRS
                     TELVTLNFGVDELMRMVGINPATAEEAMDIVLEDQNLEFKVNQIVIWNPSVLATKAKF
                     YKSRDHLSVHDEEKASSRVAERPGSEPPPLDMESPQDDVAGGGEDLALDMSVDFDAPS
                     KKDDGELHVPSEEIDFNFD"
     gene            complement(9881..>10316)
                     /locus_tag="PKJ16_11255"
     CDS             complement(9881..>10316)
                     /locus_tag="PKJ16_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HNV47608.1"
                     /translation="TVTGSHAIPVAADRELDGVHAEDFGLIVLPGGMPGSEHLKNDAR
                     VISLVRRIHASDGIAGAICAAPIVLAAAGVLAGKQATCFPGFETELAGAIYNPKPVIL
                     DGRIVTGRGAGCAIPFALEIVGLVRGKDVMRGLAKQLQVYWL"
BASE COUNT         2264 a         3207 c         2956 g         1889 t
ORIGIN      
        1 gatctcctcc ggctccgaga gcgtgagcga cagcatggag gagcagtcgg ccgcgatgga
       61 gtcgctcgcg cggctcatcg gcgagctgac ggccatcacc gggacgatgg aggcgcgcat
      121 caccgattcg ctcgcgaaaa ccaaggagat cgcgcgccgc gcgcaggcgg gcgagacgca
      181 catcatgggg atgagccgca gcatggagga gatcaacgac acggcgcggc agatgacggg
      241 gatcctcaac atcatcaacg acatctccga ccgcatcaac ctgctgtcgc tcaacgcctc
      301 catcgaggcg gcgcgcgcag gcgagcacgg ccgcggcttc gcggtggtgg ccgacgagat
      361 cggcaagctc gccgaccaga cctccaccag cgtgaaggag atcgacgcgc tcatcaagaa
      421 gagcgagcag gagatcgggc gcggcctggt gaccgtgcag gacacggtcg agacgatgcg
      481 cggcatcatc gagggcgtga acgtatcgaa caacaccatc agcgacatca acgcatcgat
      541 ggaccagtac gtgaagtcca acgattccgt ccgcagcgag accgaccgcg ccaagcagcg
      601 ctccgacgaa atcaagaccg ccatgctgga gcagaagaag gcggccgagg agatcgtggg
      661 caccatcacc ggcgtgaaca acatctcgca ggaaaacgcc atcagcgccg aggagatcaa
      721 gcggcaggcg gagatgatcg cggacatggc gctcgacctc agggacaaga tatccaagtt
      781 cgacgaggag tgggtcgacg cgcccgccga acggttcggc ccggaggcat gagcccccag
      841 ccgcctgaaa agaccctccc cgccggaccc cgccgacggc acccggcccc gcgccggtaa
      901 aattatcatt gcgccgaaaa aaatatttga aactatggca ttccctacta tcattaatag
      961 cgggagatgg gagtaccgcc ccgtacaccg gaccgacgac tatgcccacg aacaccaata
     1021 gcgaccacga tctcgacggc gatctcgcgc ttgattcgcg cgacgaggtc gagcacccga
     1081 agatgtatcg cgtgatcatg cacaacgacc actacaccac catggacttc gtggtggagg
     1141 tgatcatcgc ggtgtttcac aagcccgcgg ccgaggcgac gcgcatgatg ttggagatcc
     1201 acaaacaggg catgagcgtc tgcggcgtgt acacctatga tattgcggcc accaagaccg
     1261 gccaggtcca ccagatggcg cgcgagcgcg gattcccgct caagtgcagc ttcgaggagg
     1321 cctgatcaag gagcgaccat ggacatcagc gacgaactca accagatact cacggccgcg
     1381 tacaacgagg ccaagaaccg caagcatgaa tacctcacgc ccgagcacgt cctctacgcg
     1441 tcgctcttct tctccggcgc caaggagatc atcgagaact gcggcgggaa cgtggaccgg
     1501 ctcaagacgt cgctcgagaa gcacttcgag aagatgatca cgccgctgcc cgacggggcc
     1561 gaccccgcgc agtcggtggg ctttcagaac gtgctcgagc gcgcgatctt ccacatcacc
     1621 tcggcgcaga aggacctgct ggatatcggc gacgtgtacg tgtcgatctt cgacgagcag
     1681 gagtccttcg cggcctattt cctcaagcgc gaggggatca cccgcctcga cctcctgaac
     1741 tacgtctccc acggcatctc cgtcgttccc agtggcgctt ccgccggcga cgccgcgccg
     1801 gagatcgacg acgaggatga ggcggcggcc ggcgacaaga aaaaggaccg aaaagcgctg
     1861 cagcttttca ccgcggagct cacgcagaag gcgaagcagg gcgagcttga gccgctcatc
     1921 ggccgcgagg acatcctcga gcgcacactc caggtgctct gccggcgctt caagaacaat
     1981 cccattcacg tgggagaccc gggcgtgggc aagaccgcga tcaccgaggg gatcgcgcag
     2041 cttgtcgcgg acggacgcgt ccccaagccg cttaaaagcg cgaagatatt ttcgctggac
     2101 atgggggcgc tcctggccgg cacgaagtat cgcggcgatt tcgaggagcg cctcaagcgg
     2161 gtgctccagg agctgggcga gcagaagaac gtgattctgt tcatcgacga gatccacacg
     2221 gtcgtgggcg ccggtgcggt gtcgggaggg tcgatggacg cctccaatat tttaaagccc
     2281 gccctcgcct ccgggaagat ccgctgcatc ggctccacca cgtacgagga gtaccgcaag
     2341 ttcttcgaca aggaccgcgc cctctcgcgc cgcttccaga agatcgagat ccccgagccg
     2401 accatcgacg agacgcaccg gatcctgcag ggcctgcgcg aaaaatacga gacctaccat
     2461 gaggtgacct acacggacga ggcgctgcgc gccgcggccg agctctcggc gaagttcatt
     2521 aacgaccggc acctccccga caaggcgatc gacgtaatcg acgaggccgg cgcctacgcg
     2581 cgcatgaacc gcgtcgagga cgaggcgaag aacgaaatca ccgcgcacga catcgagcgc
     2641 gtcgttgcca agatcgcgcg cattccggaa aagagcgtgt ccgcgccgga gaccgtccgg
     2701 ctcaaggatc tggagcgcga gctcgcgagc cagatattcg gccaggacaa ggccgtcgcg
     2761 gccgtggccg aggcgatcaa gcgctcgcgc gcgggcttcc acgagaccga caagcccgtc
     2821 gcgtcgttcc tcttcgtggg gccgaccggc gtgggcaaga ccgagcttgc gcggcagctc
     2881 gcgcgcatcc tgggcatcgc catccatcgc ttcgacatga gcgagtatca ggagaagcac
     2941 accgtcgcgc ggctcatcgg cgcgccgccc ggctacgtgg gatacgagga gggggggctc
     3001 ctcaccgagg cgatccgcaa gaccccgcac gcggtgctcc tcctcgacga aatcgagaag
     3061 gcgcacacgg acatcttcaa cacgctgctg caggtgctcg actacgcgac gctcaccgac
     3121 aacaacggca agaaggccga tttccgcaac gtgatcatca tcatgacctc gaacgccggc
     3181 gcgcgggaga tcgggcgcaa gaaggtggga ttcgacgagc gcgtcgtgaa ggacgacgcg
     3241 atcaccgagg ccatcgagcg cttcttctcc ccggagttcc gcaaccggct cgacgcgatc
     3301 gtaaacttca acaacctcga cgaggagatc gcggtgcaga tcgtccgcaa ggagctcgcc
     3361 gagtttcagc gacagctcga cgagaagaag atcgcgctca ccgtcaccga cgagtgctgc
     3421 cgctggctcg cgaagcgcgg cttctcgccc gtattcggtg cgcgcgagat cgcccgcgtg
     3481 gtgcgggaga agatcaaaaa gcgcttcgtg gacgaggtgc tcttcggccg gctcgcgaac
     3541 ggcggcgcgg tgcgtatcga cgtcaaggac gacgaggccg tgttcgacat cgcctcgtag
     3601 cgcccatgcc ggtctatcag ctctcggagg cgatcgcctt cccgcaccca tcgctggcca
     3661 ccgacgaggg agtgctggcc gtgggcggcg acctttcgcc ggagcggctg cttttggcct
     3721 acgccaacgg gatcttcccc tggtattcgc acggggaccc catcatctgg tggagccccg
     3781 acccgcgctt cgtgctcttt ccgggcgagc tcctggtctc caagaccatg cgccaggtgc
     3841 tgcgccgaaa cgtgttcacc ataacgttcg atcgcgcctt ccgcgcggtg atcgcctcct
     3901 gcaagaagat ccggcggcgc cgggagcgcg gcacctggat cacgggcgag atgctcgagg
     3961 cctacgtgcg cctgcacgaa ctgggctatg cgcattccgc ggaggcgtgg cgcgacgacg
     4021 agctcgtcgg gggcctctac ggcgtgtcgc tcggccgctg cttcttcggg gaatcgatgt
     4081 tctcacgcga aagcaatgcc tccaaggccg cgctcatcac cctcacgcgc cgcctggccg
     4141 cgcgcggctt tggccttgtg gactgccagg tgcacaccga tcacctcgag tcactcggcg
     4201 cgcgcgagat cccgcgcgac gaatttctgg tcaccctgcg cggactcgtg ggcgcggaca
     4261 cgctgcgggg cgactggggg gccctgatgc ccgtcgagga ccgctgatca ctccgcgttc
     4321 cctcgacatc gcccgggaca cgcctccgcg ctccatcatc ccaccgaaaa ccggttgacg
     4381 cgaaacgacg ttcgtgccat cgtcaaacgc cgttcgccgc gatttccgtt cccggagtgt
     4441 tcgccatgat caaggacaat gcagcagaaa aaatcaagca catcaaggag atgggccgat
     4501 ccgtctcgat tttcgaccag aagatcttcg gggtgatcgt cggcgtcgcc gccctcatcg
     4561 tcgtcgcgct ggtcgtgtac ttcgtcgatt tctcgccaag cctctcgcac ctgcgcgtga
     4621 cggtcttctc cggcgcgacg accggcaatt accacgccac cgtgaccacc gcggccgata
     4681 tcgcgcgacg ccggggcggc aggatcgaga atatcgcgac caacggctcc atcgacaaca
     4741 tcaagcgcct gcagcagacg aagaagcgcg gccagttcgc gctcgcccag aacggcatgc
     4801 cgtgggggga gggcatcgag ctcgtcgcgc acctgcgcgc tccggagacg gtgttttttc
     4861 tgggaccgaa cgccgattcg atcaaaacgc tcgcggacct gaagcacaag cgaatcggaa
     4921 tcggcccaaa agggagcgga accgcgcacc tggccgagac catcttcaat acgccgcaat
     4981 tcgcgggact tgcggtgtcg ctctcgcacc actcgtccga cgagcagctc gcgctcctca
     5041 aacagcgaac gatcgatctt ggcgtgttcg tgattagcga gaaatccgtt ttcatcgaga
     5101 aggcggtctg cgaagacggc atgcagatcg caagcttccg gcagagcgaa agcatcgcga
     5161 tgcgcctgcc cttcttgaaa tcggactaca tccgcgaagg actgtatgat ccggtgcgca
     5221 acctccccgc ctccagcaag aaggtgctca agctggacac gctgatcgtg tcgaacggcg
     5281 aggcgaaacg gtcgcaggtg atcggactgc tctcggtgat gagcgacatc tacccgaacc
     5341 tgatcaacta caatcgcacg gtgaccaact acaccggcct tcccgagtcg tcggccgcgc
     5401 gggatttctt caacaacacc ggcccggaaa ttctcgatcg ctactcgccg cggctgatgg
     5461 acatcctgcc gctctcgaac ctggtgcagc tcgcgatggc catcagcatc ttctttaacc
     5521 tgatgggcgt cggcaaccga tttttcctct ggcgcatcga cgcaaaccgg atcgcgctcg
     5581 aggtggaaat gcgcgatttc ttcggacgcg gcatccttcc cgaggagatc gagcacatgg
     5641 ccgcaatgga ggagcatcgc accgacgacc ggcgccggtc gctcacggcg ctgatcgagc
     5701 ggctggaaac gctcgagggg cgatgccgca aacagtcgca ggggctcctg gtgcccatgg
     5761 gcggcgagat ggcgtatcgc tatcaggagg agatcatcag ccgaaacctg gttgggctgc
     5821 gggcgtatcg ggacaagatt gcgtgaggtg gtaggatcga tgtgggggac aagagggctc
     5881 tgaccccgta ggattgctgg caaaaggctc tgaccccgga cattacgaac atcacacaag
     5941 ggtcagagcc ccaagcacat acttcgtcgc tagcataacg caaacaaagt gagtattatg
     6001 gctacatatt cctccactat catctttccc accaaaggaa atcgagacat aattgacatc
     6061 accgataaga ttcaagctgc gataaacgag agtggcgcat taaacggggt cgttgtcgct
     6121 ttcattccag gctccacggc agggcttacc accatcgagt acgagccagg cttaaaaaaa
     6181 gacatgaatc tgttccttga gcgaattcta ccacacgggg atcggtatca tcaccatgac
     6241 acctggcatg atgataatgg cgccgcccac ctgcaagccg ccatgatcgg accttccatc
     6301 accatcccgg ttgtcaatgg gaaaatgaca ctcgggacat ggcaacaggt ggtttgtatt
     6361 gactgcgaca ccaggccgcg gcaacgcgag attgtgcttc agattgtctc ctaatggctc
     6421 acctgatggg tgcctagggg ctctgacccc tgactcttcc atccaacgct tcccataaat
     6481 acttcaacaa tcatcctgaa aaattcttca atagaacgaa ttattcaaaa gtttcctgat
     6541 ccgcccgtaa tacatataga tgccagatat taccatagcg cgaaaaggag gacaatatgc
     6601 cacgcccgct acgcaaacat atcgaagacg ccacgtatca catattcacc cgatgcatca
     6661 acagcagcga tctcatcaga aatgacgcat ataaggaaat attgttaaaa atcttacagg
     6721 caacacaaga gaaatacgct ttcgatcttg tgttttacca aatcctcgac aatcacatcc
     6781 atcttgtgat acgaacacgc aagggagaag caagcatttc aaggataata caatacttca
     6841 aggcgaggtt tgccgagcag ttcaatcgga aaaacaacag gaccgggcca ttctggaacg
     6901 aacggttcgg cgacgtaatc gtcgacctcc aggagaaccc ggtgttctac ctcttgtggc
     6961 tgctttggta tctagcgtat aatgccgtga ggaaaaacaa ggttcgggat ccgcgaaaat
     7021 accaatatgg gagcatctca cagtatctcg accaagattc atgcaacaat gggaaagtac
     7081 gcattacgca ccatcaattc tttattaatc ttggggacag ttttacagaa cgggtaatga
     7141 gatttctttt aattgaggat atgtaccgaa aacgattggg gttgccagtt taaaaagcgt
     7201 ggggtcagag cccttgattc tcatggcgcc tttccatgcg ccggcgctcg aacaacaacc
     7261 tccttgccgt tctcaaggac cactgccgtt ccattcaaaa agtcggacgc atactcgaac
     7321 cgtgcgggaa tcacaaacga tcccgctcga tcaatgtatc cccacttgcg atcgcttccg
     7381 ccgtacatga tcatcaccgc ggccaaaccc tcggagaagt tccttgcatc gtcgaattgc
     7441 gttcggatcg cgagccggcc cttggggtcg ataaacccat atttgcccga tacgcggata
     7501 agcgcacgcc catcgtgaaa ggggccggca aactcgaacc gcggattgat gacaaacgaa
     7561 tcgttgtgat cgacaaagcc ccaccggaga ttatccccaa ccttgggaac aagcggggct
     7621 ctgacccctt tttccacgcc ccctgccgcg cccgcaagac ccaggacgac agcacacagc
     7681 gcccaacgga acacccatgt tcgcacagtc ttccccatca tgagcatcac gctcgcttct
     7741 tcttgcgttc gtattcgcgc atatactcat cgatcaggct cttgctctgg ggctcttcgt
     7801 ccacatgctt catcttacgg ccaaccatct ccgcagccgg tttaaagagg ccggcgaaaa
     7861 aatccttgat ggaccggaag agctcagaca ctccctcgag cgccctgcgg atgatggaca
     7921 ccttccctcc cgacggcgcc ttgcgcagaa tctgcgacac atcggccacc ttcgcggtgg
     7981 tcgccgtaac cgtttcggcg accttcttca ccggctgggc gccgtacatg cccgggatgg
     8041 gattcacgac atctttcacg taggcctttg ccggcgtata ggccacgcgc tggggaatct
     8101 ttttccagaa gataatgcgc gattgcgatt ccgtcgcaga acggtcgagc ataaagagaa
     8161 agagatagta aaaatacatg aaaaaattaa agaatatgag gaagaagcac tgccagatct
     8221 cgaagaaggc ccaccacacg tcgatccaga tcttaaaaaa gtctacaatc ttataaaaga
     8281 aattgtagaa attatcaacc gaattgttcc agaactgctt aacgatcgta taattcataa
     8341 tgcgctcctg acagcatatc cgtatggccg catttttctc ggcccgcaat ttcccaccgg
     8401 cacaccatat ccgcacttcc tgtaaaatcg gcggctgttc ccgtcaatac aattttccat
     8461 cagtcgaagt tgaagtcgat ctcctcagac gggacatgca actcgccatc gtctttcttc
     8521 gacggagcgt cgaaatcgac ggacatgtcg agtgcgagat cctccccgcc ccccgcaaca
     8581 tcatcctgcg gactctccat atcaagggga ggaggctctg accccggacg ctcggccacg
     8641 cgcgaggagg ccttctcctc gtcgtgtacg gacaggtggt cgcgcgactt gtagaacttc
     8701 gccttcgtcg cgaggacgct cgggttccat atcacgatct gattcacctt gaactcgagg
     8761 ttctggtcct ccaggacgat gtccatcgcc tcctcggccg tggcggggtt gatgcccacc
     8821 atgcgcatga gctcgtccac gccgaaattg agcgtcacga gctcggtgct ccgcagggac
     8881 acgttgagct ccaggagctg gttttcgaga aaggcgtaaa tctgcgttat gtgcttcttg
     8941 tagagcttcg actccaggcg gatatacgta aaccaaatcc gctggctgat ggccatgaag
     9001 attttatgga tgatggtggg cgacttcgcg agcaccgtct tcagcgtgtt ccggttgatg
     9061 ggtagcgcct tggcgatccc ccagctgatg gccgaggcat tgcgcggctt gttggacacg
     9121 atcgcgagct cgccgaagat gtcaccctcc ttgagcacgg aaagcatcac ctcgttctcc
     9181 cggctcacct tcacgatctt gacccggccc tccttgatga tgtacagctc ctcgccgggc
     9241 tcgtattcgc aaaagatcat ctgccggtcc gagaacaccc gatagatccc ctcgcgcatg
     9301 ggttcgatga tgccccgatg gccggttttc tcgatctcgg cgatcatctc catcgcggca
     9361 gggcggttaa aggcgccggg gtactgcccg gcatagcgct tgaggacata ataggcgtac
     9421 ggatactggt ccttccggaa ataatattcg cccaggttga agaatttcgt ttcggtggtg
     9481 ttggtgtcgg gctcctggtc gatgaggcgc agcatcatct cgtcatacac ccgcagctcc
     9541 tcggcgaagc cccggatgat tttaaatgcg atatcgggat ttttctggat gagctcgaag
     9601 aacttcgccc gcgggcatac gaccacctgc acgtcttccg cggcgacggc cgtttccatc
     9661 cgggggcggc ggcacagcgc ggagatgaag ccgaacacgc cgcccacttc gaccgtcgaa
     9721 cggaagcgct tgatcttttc gtactcgctt accagctcga tcgtgccgcg ctcgacgatg
     9781 tagatatcgt tcacatcctc gtcgccctcg atgtagatgt aggcgtcctt cctgaacgcg
     9841 cgttttttca tctgttccgg cttgttcacg ccctacccct tcacaaccaa tagacctgca
     9901 attgttttgc aagaccgcgc atcacgtcct tcccccgcac gagccccaca atctccagag
     9961 cgaacgggat ggcgcatccg gccccgcgtc ccgtgacgat cctgccgtcg aggatcaccg
    10021 gcttgggatt gtagatcgcg cccgcaagct ccgtttcgaa accggggaag caggtcgcct
    10081 gttttcccgc gagcactccg gcggctgcca gcacaatggg ggcggcgcag atcgcccccg
    10141 cgatgccgtc cgaggcgtga atccgacgaa ccagcgatat cacccgggcg tcgtttttca
    10201 ggtgctcgct gccgggcatg ccccccggga ggacaatgag gccaaagtcc tcggcatgga
    10261 ccccgtccag ctcccggtct gcggcaacgg gaatagcgtg cgatccggtc acggtg
//
LOCUS       DAORHF010000127        10104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_5959_length_10104_cov_6.746442, whole genome shotgun sequence.
ACCESSION   DAORHF010000127 DAORHF010000000
VERSION     DAORHF010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10104
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5959_length_10104_cov_6.746442"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..589
                     /locus_tag="PKJ16_11260"
     CDS             113..589
                     /locus_tag="PKJ16_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47609.1"
                     /translation="MSVATTLYVHEATLGLIAEACAMTGATRSRLIAMLLERSMRDPG
                     MEPRIGRLVRYQRADRREAWRTVHVRFREDEVDYVKDLRSFYRVSASLLLAVAARRFI
                     CEIIRQSEKREKSDNYPFSNYLLSRETIDGYIFWKICWGFPPNPEKYITIDPAPLE"
     gene            638..2035
                     /locus_tag="PKJ16_11265"
     CDS             638..2035
                     /locus_tag="PKJ16_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HNV47610.1"
                     /translation="MIRFDYNNFMSEAIGAEHGLAEADLASVEKKADAALERMAREKG
                     AGRLGFLEVPYDETIVRDIRAYKKKLGGRFDTVALIGIGGSCLGPQALKNALSHLYLN
                     ELPGAARGDRCKIYYFDNIDPFEIEAARDVLDVTKTLFLVISKSGGTVETNANFAILL
                     DMLKRAVGANYAEHIVAITDPEKGTLRTLATAEGFQTFPVPDNVGGRFSVLSAVGLVM
                     AEFTGIDAAGLLAGARNVVQRYYGRGLRQNAPLLNASFHYLFDTVKGKRINVLMPYTR
                     KLFLFADWYRQLWAESLGKNTDANGAPTVAGLTPVSALGAIDQHSQLQLYLEGPNDKV
                     ITFMKLDDFGRDVTIPEFYPDQPEMGYLSNKSLRALNRFEESATELVLAKAARPNCSL
                     VLPTLTAETVGEFIMFLEMQTAYAGYLYNINPYDQPAVEQGKKYAFGLLERPGYDEFR
                     DRFAAGYVKKDAFIL"
     gene            2032..2649
                     /gene="tmk"
                     /locus_tag="PKJ16_11270"
     CDS             2032..2649
                     /gene="tmk"
                     /locus_tag="PKJ16_11270"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HNV47611.1"
                     /translation="MRIRLPLFVLFEGIDGSGKTTLADTVARHFSAAGVPVARLGEPT
                     DGVWGRRIRALLRGNAMPPPEEQMALFLEDRADDVARNIRPALADGRLVVMDRYYFSN
                     AAYQGAAGLSPAHVIEENRARNFPEPDRVYLADVAPEEALRRIEARNAGTGATELFER
                     REFLASVRATYRALTDERFVILDAALPPDALAALVIDDMHARFGM"
     gene            2656..4119
                     /locus_tag="PKJ16_11275"
     CDS             2656..4119
                     /locus_tag="PKJ16_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV47612.1"
                     /translation="MEQPESTTTAQRPGALGESAVRRVTGARSRALNVVLTSGLDLAL
                     VAILGGLSAACISQGLSGPYLVLFFMVLGVSLLPLFSFFHNTIDSIISPIDYHELFAQ
                     TVDSILRIESFDAVLTATFDRILRLINVKSGMLIFFYHDRDEFNIFYQKGRRRRVIKG
                     ARIEENNVLFTALRGPDDIIVKHRLDPKVNHQREIVDELERLGGEVAIPIFYRDMFLG
                     LILVGDRMRRFSPGEIRLLRIFASRIATLSTSGFFFGQLVEKMRLEKEFELASKVQNR
                     FLPDADLDAGAVRVRVHHRTASLLVREFYDVFAGDDGAVRISAYRVEENISGTSIYLP
                     WIQASIHSLSRLGYPPSRIIARVKRIIADKGLMDEPVSIIHAVLRQNGSCAFAVDGYQ
                     TPLVYRAASRTLAPAERSARSAQSSLRLAPGDALLVACRDIRALINAAPARYAEIIAA
                     NAPSAGRVRSALVKQLAEADDGAGDRLLIVCTAGRGA"
     gene            4116..6494
                     /locus_tag="PKJ16_11280"
     CDS             4116..6494
                     /locus_tag="PKJ16_11280"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV47613.1"
                     /translation="MSVRAGAAAVLIVLVGLNFLFVPRAVRYAGLSPFARVSVDGSFF
                     HRIGPRDEGIGFRSAVLAIGDAEIAPGALPAALVSREGERTLSMRLADARGDVYERMI
                     VKWSVNEPLAWLYLVLFLVANVHWLWGFAVWVKNPDSTQARLYCAGSLCLGVVCFSIA
                     DLFSYGTAVPLLMAASVLLGYCVIEIAYALSGGRFGARLILSYFGACAVLLAAYLYEP
                     APLAGGTRGALLFAPPVVSVLLFIVKIGVYGARQRNAYTLRSAGVVVTAFTAGILAPF
                     GALIAGFFWDLPVPAHVPVAASVLIPMLVGNGLMRPMLFSTGARFGGGFALFVINGAI
                     ALCAALSLLYIHNVFRSWSDAALLYPLFGVMMLVLLYFRRIVNRRLNDILFVTRDDYA
                     NCLQRIAEHVASPDESAVKLERAFAAVRETAGVAACSVAIFDAGTDHAFERSGGLLER
                     MDADSPPGRYFAKHTGMAFRYSIAPGPGADGEALAFMRARNLALMMPVFDGGRLRVAL
                     LVGEKRGGRLFVYEDIRFFGTVAFQAYQMLENDRLFRDYLVRTAYEKELDVASYVQLR
                     LFPKRTPQRRGLAITFYNRPFFKVTGDYFDFVTIDKSRTAIIIGDISGHGLSAAMVLS
                     MMSSIIATLLAERKPIGDIMDEINGYLTRRYRGTDLITLFIGVYHKQTRELTYINAGH
                     CPPAHIRRTGEVSLMEGRAKILGADPKARYAESALTLARGDELVLYTDGVIEIFDEAK
                     NEALDEGDLLAIIRQNRLSDTERKVAAILERINRFPNECITDDITLIGVTVL"
     gene            6445..8952
                     /gene="sppA"
                     /locus_tag="PKJ16_11285"
     CDS             6445..8952
                     /gene="sppA"
                     /locus_tag="PKJ16_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV47614.1"
                     /translation="MNALRTTSPSSASRFSNALRAVALALALAAAAPVAAGEVRNPFE
                     APFAGTPASHANSEFAASFNPVFADVLMAPSLSYRLQYFDGQSGTDHLFHLNLAGFAL
                     TYGLFNALFDRENERVASARADYFSVGKGLMFMNVFGVGAAYAFSKSSAAAYDGYGGL
                     SFSALFRPFDFLSFGVIARDVNAPELDGRRVVSSQVYSVSLRPWGEFVTLSLDAIHRG
                     AERVDECNVVFAADVRAWREATVFASVDREGSFSFGARMPIDLTRESARTVVPEANRL
                     QGRHGLRLSQAGLTLSGERYASPVTIFTRSLAITLNEPYPESAEERFLEKPRARFYDL
                     LAAIRAAREDPTIGAITLSVNQAAYGVAQIQEIRAELLAFRQSGKRVYAALSEMSNRH
                     YYLATAADEIAFVPASSFYLTGLKAEVYFFGAALGKAGVKYEGISRGRYKSLNEQFMR
                     ERMSPAHRSNLVSIIGELNRQFMDDIAASRRITRRDLDRFVARGVATPEEARQAGLIN
                     SIEYPAAALRRMAKRTVPSGVSVSLESYLAEARRMERWGPVPAVAVVYVEGAIRKGES
                     RGRGLMTPDVIGDETYRKDLARAFGDPSVRAVVVRVNSGGGSALASDLMWRALTEKKE
                     ATKKPVVISFGNMAASGGYYIACTGDAIFVSAGTLTGSIGVVGGKVSLARLYRTLGIN
                     KDVVKMSEYADLLNEARDLSVRDRAQLDRLIGLIYRQFTDRVSAGRSIARADIPRVAE
                     GRVFTGSQALGNKLADELGGLARAVDRAKRLAGIVGPCMVRHLPERHSSFKDLVAGPR
                     IEAPVARELTPIVESLRALGWGSEPVLYLYPYIVVIE"
     gene            complement(8974..9801)
                     /locus_tag="PKJ16_11290"
     CDS             complement(8974..9801)
                     /locus_tag="PKJ16_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47615.1"
                     /translation="MKPFTIIITIGISVITLIYSSVPACASDASIEREAASRICEICG
                     TKNRSRLAIYPFTDATDKETEATKKTATRLISLVLQCGRIKIVDPSKVAAILEEQEKG
                     MTGAVDDQTAPKAGKLLGADALLFGQAAAGSLQIRIVDATTGEILGATVTERGASGKP
                     AVQIEQFDKEAARLDFRRQQLRKHLAFLFHRRPLIYLFMTATDDELSQLEKNNPRLYR
                     SVRERLDGSSDDQKKKFDASRSMVLELRRTDASQNRRIIESHQDIRRRLQERRPRNR"
     gene            complement(9877..>10104)
                     /locus_tag="PKJ16_11295"
     CDS             complement(9877..>10104)
                     /locus_tag="PKJ16_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010876083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47616.1"
                     /translation="VYRVRDNGVGFDMKQVDKLFSVFQRLHGQEEFEGTGVGLSIVQR
                     IVLQHGGRVWGEGKRNRGATFCFTLPVVSTI"
BASE COUNT         1596 a         3250 c         3308 g         1950 t
ORIGIN      
        1 agataattga cgccgagacc gtcgttgccg ggtgcgctgc gcgaggtttt tttgttccgg
       61 ttttcccgtt tggtccgtat gtacttgtag tacgttccat tcgggaggga ccatgtctgt
      121 tgcgacgacg ttgtatgtgc atgaggccac gctggggctc atcgccgagg cgtgcgccat
      181 gaccggagcc acgcgcagcc ggttgatcgc gatgctgctc gagcgctcca tgcgcgatcc
      241 ggggatggag ccgcggatcg ggcgcctggt gcggtatcag cgggccgatc gcagggaggc
      301 gtggcgcacg gtccacgtgc ggtttcgaga ggacgaggtg gattacgtga aggacctccg
      361 gtcgttctac cgggtttcgg cgtcgttgct tttggcggtg gcggcacgac ggtttatttg
      421 tgagatcatc aggcaatcgg agaagaggga aaaaagcgat aactatccgt tttcgaatta
      481 tctcttgtcg cgtgaaacga tcgacggcta tattttttgg aagatatgct ggggattccc
      541 cccaaacccc gaaaaataca tcaccatcga tccggcgccg ctcgaatgat ggcggccgcg
      601 ccgcatatcc cgacaagaca tcaataggag gagcaccatg atacggttcg actacaataa
      661 cttcatgagc gaggcgatcg gcgccgaaca cggccttgcc gaggccgacc ttgcgtccgt
      721 cgaaaaaaag gccgatgccg ctctggagcg catggcgcgg gaaaagggcg cggggcggct
      781 cggatttctg gaagtgccgt atgatgaaac gatcgtacgc gacattcgcg cctataaaaa
      841 gaaactcggc gggaggttcg acaccgtcgc gctgatcggc atcggcggct cgtgcctcgg
      901 cccccaggcg ctcaagaacg cgctctcgca cctgtacttg aacgagcttc cgggcgcggc
      961 gcgcggcgat cgctgcaaga tatactactt cgacaatatc gatcccttcg agatcgaggc
     1021 ggcgcgggac gtgcttgacg tgacgaagac cctctttctg gtgatttcga aatcgggcgg
     1081 gaccgtcgaa acgaacgcca atttcgcgat cctcctcgat atgctgaaac gcgcggtggg
     1141 cgcgaactac gccgagcaca tcgtcgccat caccgatccg gagaagggga cgctgcgtac
     1201 gctcgcgacc gccgagggct tccagacctt tccggttccg gacaacgtgg gcggccggtt
     1261 ctcggtgctc tcggcggtgg ggctggtgat ggcggagttt acgggcatcg acgccgcggg
     1321 gctcctcgcc ggggcgcgca acgtggtcca gcggtattat ggaaggggcc tgcggcagaa
     1381 cgcgccgctt ctcaacgcct cgtttcatta tctgttcgat accgtaaagg gcaagcggat
     1441 caacgtgctc atgccgtaca cgcgaaagct ctttctcttc gccgattggt atcggcagct
     1501 ctgggccgag tccctgggga aaaacaccga cgcaaacggc gccccgacgg tcgcggggct
     1561 cacgccggtg tcggcgttgg gcgcgatcga ccagcactcg cagctccagc tctatctcga
     1621 ggggcccaac gacaaggtga tcaccttcat gaagctcgat gatttcgggc gcgacgtcac
     1681 catccccgag ttctatccgg accagccgga gatggggtat ctgtcgaaca agagcctgcg
     1741 cgcgctcaac cgcttcgagg agtcggccac ggaactcgtg ctcgcaaagg ccgcgcggcc
     1801 caactgctcg ctcgttctcc cgacgctcac ggcggagacg gtcggcgaat tcatcatgtt
     1861 cctggagatg cagaccgcat acgcggggta cctgtataat atcaatccct acgaccagcc
     1921 cgccgtcgag caggggaaga aatacgcctt cggactgctc gagcggccgg ggtacgacga
     1981 gttccgggac cggttcgcgg cggggtacgt caagaaggac gcgttcatcc tatgaggatc
     2041 cgcctgcccc tctttgtcct ctttgagggg atcgacgggt cggggaagac gacgctcgcc
     2101 gataccgttg cgcgacactt ctcggcagcg ggcgttccgg tcgcgcgcct gggcgagccc
     2161 accgacggcg tctgggggcg tcggatccgc gcgctcctgc gcggaaacgc catgccgccg
     2221 cctgaggagc agatggcgct cttcctcgag gaccgcgccg acgacgtggc gcgaaacata
     2281 cgcccggccc tggcggacgg ccggctcgtg gtgatggacc ggtactattt ttccaacgcg
     2341 gcctatcagg gcgcggcggg attgtccccg gcgcatgtta tcgaggagaa ccgcgctcgg
     2401 aatttccccg agcccgatcg cgtctatctc gccgatgtcg cgcctgagga ggcgcttcgc
     2461 cgcatcgaag cgagaaacgc gggaacgggc gctactgagc ttttcgagcg tcgggagttt
     2521 cttgcgtcgg tgcgcgcaac ctatcgcgcg ctgaccgacg agcgatttgt gattttggac
     2581 gccgcgctgc cgcccgatgc gctcgcggcg ctggtgatcg acgacatgca cgcgcggttc
     2641 ggaatgtagc gattcatgga gcagccggaa tcaaccacaa cggcgcagcg gccgggcgca
     2701 ctcggcgaat cggccgttcg gcgcgtgacg ggcgcgcgct cacgggccct caacgtcgtt
     2761 ctcacgtcgg gactggactt ggcgttggtg gcgatactcg ggggactctc cgccgcatgc
     2821 atctcccagg gcttgagcgg cccgtacctg gtgctgttct tcatggttct gggcgtgtcg
     2881 cttttgccgc tgttcagctt cttccacaac accatcgatt cgatcatcag ccccatcgac
     2941 tatcacgagc tcttcgcgca aacggtcgat tccatactcc gtatcgaatc cttcgatgcg
     3001 gtgctcaccg cgaccttcga ccgcatcctg cgcctcatca acgtgaagtc ggggatgctc
     3061 atcttcttct atcacgaccg cgacgaattc aacattttct atcagaaggg gcgccggcgc
     3121 cgggtgatca agggcgcgcg tatcgaggaa aacaacgtcc tgttcacggc gctccgcgga
     3181 ccggacgaca tcatcgtgaa acaccggctg gacccgaagg tgaaccacca gcgcgagata
     3241 gtggatgagc tcgagcggct gggcggcgag gtcgcgatcc ccatcttcta ccgcgacatg
     3301 tttctcgggc tgatcctcgt gggcgaccgc atgcgccgct tttcgccggg cgagatccgg
     3361 ctgctgcgca ttttcgcgtc gcgcatcgca acgctctcga ccagcggttt cttcttcggc
     3421 cagctcgtcg agaagatgcg cctcgagaag gaattcgaac tcgcctcgaa ggtccagaac
     3481 cgcttcctgc ccgatgcgga cctggacgcg ggcgccgtcc gggtccgcgt gcaccaccgg
     3541 accgcgtcgc tgctggtgcg cgagttctac gacgtcttcg cgggcgacga cggcgctgtc
     3601 cgcatctcgg cctatcgcgt cgaggagaac atctcgggga cctcgatcta tctgccgtgg
     3661 atccaggcgt ccattcacag cctgtcgcgt ctcggctatc cgccgtcgcg catcatcgcg
     3721 cgggtgaagc gcatcatcgc cgacaagggc ctcatggacg agccggtgtc catcatccat
     3781 gccgtgctgc ggcagaacgg ctcgtgcgcg ttcgccgtcg acggctacca gacgccgctg
     3841 gtgtatcgcg ccgcttcgcg caccctggcg cccgccgagc gctccgcccg gagcgcgcaa
     3901 tcatcgctgc ggctcgcccc cggggacgcg ctgctggtgg cctgtcggga catccgtgcg
     3961 ctcatcaacg ccgcgccggc ccgatacgcg gagatcatcg ccgcgaacgc gccgtcggcg
     4021 ggccgcgtcc gttccgcgct tgtcaagcag ctcgccgagg ccgacgacgg cgcgggcgac
     4081 cgcctgctga tcgtatgcac cgcggggagg ggcgcatgag cgtgcgtgcc ggagccgcgg
     4141 cggtcctgat cgtgctcgtg ggactcaact tcctgttcgt tccccgcgcg gtccgctatg
     4201 cgggcctctc gccgtttgcg cgcgtatcgg tggacggctc gttctttcat cggatcggcc
     4261 cgcgcgacga ggggatcggc ttccggagcg cggtgctggc gatcggcgac gccgagatcg
     4321 cgccgggggc cctccctgca gcgctcgtct cccgcgaggg cgagcggacg ctttcgatgc
     4381 gcctcgccga cgcccgcggc gacgtctacg agcgcatgat cgtcaagtgg tcggtgaacg
     4441 agccgctcgc ctggctgtac ctggtgctct ttctggtggc gaacgtgcac tggctctggg
     4501 ggttcgcggt gtgggtcaaa aaccccgact cgacgcaggc gcggctctac tgcgccgggt
     4561 cgctgtgcct cggggtcgtc tgtttctcga tcgccgacct cttctcgtac gggacggcgg
     4621 tgccattgtt gatggcggcc tccgtcctgc tcgggtattg cgtgatcgag atcgcctatg
     4681 cgctctcggg tggccgattc ggcgcgcggc tgattctgtc gtacttcggg gcgtgcgccg
     4741 ttctcctggc ggcgtatctg tatgagccgg cgccgttggc gggcgggacg cggggcgcgc
     4801 tcctgttcgc gccgccggtc gtctccgtgc tgttgtttat cgtaaaaatt ggcgtgtacg
     4861 gcgcgcgtca gcgcaacgcc tatacgctgc gcagcgccgg ggtggttgtg accgccttca
     4921 cggcggggat cctcgcgccc ttcggcgcgc tcatcgcggg attcttttgg gatcttccgg
     4981 tccccgcgca cgttcccgtc gccgcgtcgg tcctcattcc catgctcgtc ggaaacgggc
     5041 tcatgcggcc catgctcttt tccaccgggg cgcgcttcgg cgggggcttc gcgctgttcg
     5101 tcatcaacgg cgccatcgcg ttatgcgccg cactgtcgct gttgtatatt cataacgtat
     5161 tccggtcatg gagcgacgcg gcgctcctct acccgttgtt cggggtgatg atgctcgtcc
     5221 tcttgtactt ccggcgcatc gtcaaccgcc ggctcaacga catcctcttt gtcacgcggg
     5281 acgattacgc gaactgcctg cagcggatcg ccgagcacgt ggcctcgccc gacgagagcg
     5341 cggtgaagct cgaacgggcg ttcgccgccg tccgggagac cgccggcgtg gccgcgtgct
     5401 ccgttgcgat cttcgacgcc ggaacggacc acgccttcga gcgctccggc ggcctgctcg
     5461 agcgcatgga cgccgattcg ccgccgggac gctattttgc caagcatacg ggcatggcgt
     5521 ttcgctattc gatcgcgccg ggacccggcg ccgacggcga ggcgctcgca ttcatgcgcg
     5581 cgcggaacct cgcgctcatg atgccggtgt tcgacggcgg acgcctgcgc gtggcgctcc
     5641 tggtgggcga gaagcgagga gggcgcctgt tcgtgtacga ggacattcgt ttcttcggca
     5701 cggtggcctt ccaggcatat cagatgctgg agaacgaccg gctctttcgg gattacctcg
     5761 ttcgcacggc ctatgaaaag gagctcgacg tggcctcgta tgtgcagctg cgcctgtttc
     5821 ccaagcgcac gccgcaacgg cggggcctcg ccatcacctt ttacaaccgc ccgtttttca
     5881 aggtgaccgg cgactacttc gatttcgtga ccatcgacaa gagccggacg gcgatcatca
     5941 tcggcgacat ctccgggcac ggtctctcgg cggccatggt gctttcgatg atgagcagca
     6001 tcatcgccac gctgcttgcc gagcgcaagc ccatcgggga catcatggat gagatcaacg
     6061 gctatctcac gcggcgctac cgggggaccg atcttatcac gctgttcatc ggcgtctatc
     6121 acaagcagac gcgcgagctc acttatatca acgccgggca ctgcccgccg gcgcacatcc
     6181 ggcgcacggg cgaggtgtcg ctcatggagg gccgcgcgaa gatcctgggc gccgacccca
     6241 aggcgcgcta tgccgagagc gcgctcacgc tcgcgcgcgg ggacgagctc gtcctgtata
     6301 ccgacggggt gatcgagata ttcgacgagg cgaaaaacga ggcgcttgac gagggtgatt
     6361 tgcttgcaat tatcaggcag aaccggctca gtgataccga gcgcaaagtc gccgccatcc
     6421 tcgagcggat caaccggttt ccgaatgaat gcattacgga cgacatcacc ctcatcggcg
     6481 tcacggttct ctaacgcact ccgcgccgtc gcgctcgcgc tcgcgcttgc tgcggccgcc
     6541 ccggtcgccg cgggcgaggt ccgcaatccc ttcgaggcgc ccttcgccgg aaccccggcc
     6601 agccacgcga attccgagtt cgccgcatcg ttcaatccgg tcttcgccga cgtcctcatg
     6661 gcgccgtcgc tctcgtaccg cctgcagtat ttcgacgggc agtcggggac ggaccacctg
     6721 tttcacctga atcttgccgg gtttgcgctc acctacggac tcttcaacgc cctcttcgac
     6781 cgggagaacg agcgcgttgc gtccgcgcga gccgattact tcagcgtggg caaggggctc
     6841 atgttcatga atgtcttcgg cgtcggcgcc gcatatgcct tcagcaaaag ctccgcggcg
     6901 gcctacgacg ggtatggcgg cctctccttt tccgcgctct ttcggccctt cgattttttg
     6961 tccttcggcg ttatcgcacg cgacgtgaac gcaccggagc tcgacgggcg tcgcgtggtc
     7021 tcctcgcagg tctattcggt gtcgctgcgg ccgtggggag agttcgtaac gctgtcgctg
     7081 gatgcgatcc atcgcggcgc cgagcgggtc gacgagtgca atgtcgtctt cgccgcggat
     7141 gtgcgcgctt ggcgggaggc gaccgttttc gcgagcgtgg accgcgaggg atcgttctcg
     7201 ttcggcgcgc ggatgcccat cgatctcacg cgggaatccg cgcggaccgt cgtgcccgag
     7261 gcgaaccggc tgcaggggcg gcatgggttg cgcttgagcc aggcgggcct cacgctctcc
     7321 ggggagcggt atgcgtcgcc ggtcacgatc ttcacgcgct cgctcgccat caccctgaac
     7381 gagccgtatc ccgaatcggc cgaggagcgc tttcttgaaa aaccgcgggc ccgattctac
     7441 gacctgctcg cggcgatccg cgccgcgcga gaggatccca cgatcggcgc catcacgctc
     7501 tcggtgaacc aggcggccta cggcgtcgcg caaattcagg agatccgcgc cgagcttctc
     7561 gccttccgcc agtccggcaa gcgcgtatac gccgcgctct ccgagatgtc caaccgacat
     7621 tactatctgg ccacggcggc cgacgagatc gcgttcgtcc cggcctcgag cttttatctc
     7681 acggggctca aggcggaggt ctatttcttc ggggcggcgc tggggaaggc gggcgtgaaa
     7741 tacgagggca tatcccgcgg gcgctacaaa tcgctcaacg agcagttcat gcgcgagcgg
     7801 atgtcgccgg cccaccggag caacctcgtt tcgatcatcg gcgaactcaa tcgacagttc
     7861 atggacgata tcgccgcgtc ccggcgcatc acgcggcgcg atctcgaccg ctttgtcgcg
     7921 cgcggcgtgg cgacgcccga ggaggcgcgg caggcgggcc tcattaattc aatcgagtat
     7981 ccggccgcgg ccctgcggcg catggcgaag cgcacggtcc cgtcgggcgt cagcgtgtcg
     8041 ctcgagagct acctcgccga ggcgcggcgg atggagcgct ggggcccggt tcccgcggtg
     8101 gccgtcgtgt acgtcgaggg cgccatacgg aagggcgagt cgcgcggtcg ggggctcatg
     8161 acgccggatg tcatcggcga tgaaacctac cggaaggacc ttgcgcgggc gttcggcgat
     8221 ccgtccgtgc gggcggtggt cgtgcgcgtc aactccggcg gcggctcggc gctcgcctcg
     8281 gacctgatgt ggcgcgcgct cacggagaaa aaggaggcca cgaagaagcc ggtggtgatc
     8341 tccttcggca acatggcggc ctcgggcggg tattacatcg cctgcacggg cgacgcgatc
     8401 ttcgtgagcg cggggaccct gaccggctcg atcggcgtgg tgggcggcaa ggtctcgctc
     8461 gcgcggttgt accggacgct cggaatcaac aaggacgtcg tcaagatgag cgagtacgcc
     8521 gatctcctca acgaggcgcg cgatctctcg gtccgcgacc gcgcgcagct cgaccggctc
     8581 atcggcctca tctaccgcca gttcacggac cgggtgagcg cggggcgcag catcgcgcgc
     8641 gccgacattc cccgcgtggc cgagggacgg gtcttcaccg gctcccaggc cctgggcaac
     8701 aagctcgccg acgagctcgg cgggcttgcg cgcgctgtcg accgcgcgaa gcggctcgcg
     8761 ggaatcgtcg gaccctgcat ggtgcgccat ctgccggagc ggcattcctc gttcaaggac
     8821 ctcgtcgccg gtccgcgcat cgaggccccc gtcgcccgcg agcttacgcc gatcgtcgag
     8881 agcctgcgcg cgctgggatg gggcagcgag cccgtcctgt acctctaccc gtacattgtg
     8941 gtgatcgaat aacgcttggc gccccgccgt gcgctaccgg ttacgaggcc gccgttcctg
     9001 cagtcggcgt cgaatgtcct ggtgcgattc aatgattcgc cggttctgcg acgcgtccgt
     9061 gcggcgaagc tcgagaacca tgctcctcga tgcgtcgaat ttcttcttct ggtcgtccga
     9121 cgatccgtcg aggcgctccc ggacggagcg gtacaggcgc ggattgttct tttccagttg
     9181 tgaaagctca tcgtcggtgg ccgtcatgaa aaggtagatc agcgggcgtc gatgaaagag
     9241 aaacgcaagg tgctttctaa gctgctgtcg cctgaagtcg aggcgcgccg cttccttgtc
     9301 gaactgttcg atctgcacgg cgggtttccc ggatgcgccc cgttcggtga cggtcgcccc
     9361 cagtatctcg cccgtggtcg cgtccacgat tcggatctgc agcgagcccg cggccgcctg
     9421 cccgaagagg agcgcgtcgg cgccaaggag tttgcctgct ttcggggcgg tttggtcgtc
     9481 gacggctccg gtcatcccct tttcctgctc ctcgaggatc gcagccacct tgctcggatc
     9541 gacgatcttg atccgcccgc attgcaatac cagtgaaatg agtcgtgtcg cggttttttt
     9601 cgtggcctcg gtttccttgt cggtcgcgtc cgtaaaggga tagatcgcca ggcgcgaacg
     9661 gttctttgtg ccgcagatct cgcagatccg gctcgcggcc tcgcgctcga tcgaggcgtc
     9721 gctggcgcat gccgggaccg acgaatatat cagcgtgatt acggaaatac cgatggtgat
     9781 gatgatcgtg aacggtttca ctgccgcctc catgcgggaa gatgtggtgc gatatgtgcc
     9841 gatcgcgcta gtgcgtacgc gatggtgcga cgctcgttat atcgttgaga cgaccggcag
     9901 cgtaaagcag aaggtcgcgc cccggtttcg tttcccctcg ccccagacgc gcccgccgtg
     9961 ctggaggacg atccgctgga cgatggacag gcccacgccg gtcccctcga attcctcctg
    10021 accgtgcagg cgctggaaca ccgagaaaag cttgtccacc tgcttcatgt cgaagcccac
    10081 gccgttgtcg cgcacgcggt agac
//
LOCUS       DAORHF010000128         9989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6087_length_9989_cov_4.833400, whole genome shotgun sequence.
ACCESSION   DAORHF010000128 DAORHF010000000
VERSION     DAORHF010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6087_length_9989_cov_4.833400"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PKJ16_11300"
     CDS             complement(<1..239)
                     /locus_tag="PKJ16_11300"
                     /inference="COORDINATES: protein motif:HMM:NF021542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HNV47617.1"
                     /translation="MDLRAFIGGWINENPGKALGAAAGLLAGVLLFTLGILKTIVIAL
                     LIVTGYVIGKAKDDNVSVVDVVTGFFRRGRNDGDL"
     gene            complement(241..804)
                     /gene="efp"
                     /locus_tag="PKJ16_11305"
     CDS             complement(241..804)
                     /gene="efp"
                     /locus_tag="PKJ16_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HNV47618.1"
                     /translation="MVSVNDFKRGMVIKIDGDLYTVIEYQHVKPARGGAFVRSKLKSL
                     TRGSTVDKTFRGGEKVEDVRIDRQTMQYQYEEGSDLVFMDTATYEPTNIDKKFVGNIL
                     DYIKEGDEVEIMMYEDRPISIEPPTFVTLKVVYAEPGLRGDTATNVLKPVKLETGATV
                     NVPIFVNQDDVIKVDTRTGEYVERVKQ"
     gene            complement(817..1890)
                     /locus_tag="PKJ16_11310"
     CDS             complement(817..1890)
                     /locus_tag="PKJ16_11310"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HNV47619.1"
                     /translation="MTSRIERIQKTIRARKKFPYFVSSVVNVKYLTGFAGSYAAMVID
                     RDAIYFISDSRYEEYARKLLPESVNFVLQERDLCTALKSILKTAGRTRLFLEEHDLSL
                     SLFNVMKKRMRGTTLVPAGDQINEMRMVKEEGEIAVIAEAVALADRCFEHIITLAKPG
                     VLEWDLAVEIEYFYRKNGCTKTSFDSIVASGTGSSMPHYETSMTKRIAQGDILLIDMG
                     CVYQGYNSDLTRTVFVGSIAPDFRHIYDTVRRAQEAAIDAVRPGMTTGRLDAAARRVI
                     ADAGCGDRFGHSLGHGVGLEVHELPAVRPRGDIRLKKNMVITIEPGIYMPDAGGVRIE
                     DMVVVSPGGCRVLTRATKDIVII"
     gene            complement(1902..4040)
                     /gene="glgX"
                     /locus_tag="PKJ16_11315"
     CDS             complement(1902..4040)
                     /gene="glgX"
                     /locus_tag="PKJ16_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HNV47620.1"
                     /translation="MMGLVHRTSRRLEPGRFYPLGATIRDGGVNFALFSQHAHEVYLL
                     LFDRAEAPPTDVIRLENRTRFIWHVFVPGLSAGQLYGYRMRGEFNPSWGFRYNENRFL
                     LDPYARALTGKCVNADNLLLCYPPNAPGLDLSRDDRDNTSIMPKCIVVSDDFDWQGDT
                     PPEIPFERMIIYETHVRGFTAHPSSGVKYPGTYAGFIEKIPYLKDLGINAVELLPVHE
                     SYVDDFLVARGLTNYWGYNTIGFFAPESSFGSGVRPGCQVDEFKALVRALHSAGIEVI
                     LDVVYNHTAEGNELGPTMSFRGIDNTVYYILTGVDGHPSRYYMNYSGCGNSLNLSNPP
                     VIRMVLDSLRYWVEVMHVDGFRFDLASVLGREGGAFQSSASFFDAVAQDPVLCRVKLI
                     AEPWDMGTYQVGNFPIDWSEWNGRFRDTVRTFAKGDPGKVREMGWRITGSADLYGDDG
                     RTAYNSINFITCHDGFTLNDLLSYNEKHNEANLENNADGSNENNSWNCGAEGETDDPG
                     IRALRRTLAKNHVCYLLLAQGVPMILGGDECLRTQHGNNNAYCQDNEISWFDWDLTRR
                     NADMRDFVKKAIAFRSRYTILQRRKFLTGQDWDRDGMPDITWYGVDGGPLRWDDPELR
                     TLCYRLDGSEEPSPLGDYALFIVLNADYRPNTVRLPDPIAGSRWLRVIDTDKPAGEDF
                     LEPGREIELSDQGSYCVQPRSTVVLLSLHG"
     gene            complement(4037..5980)
                     /locus_tag="PKJ16_11320"
     CDS             complement(4037..5980)
                     /locus_tag="PKJ16_11320"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HNV47621.1"
                     /translation="MASAFKGYMGRIMDVDLSNGRIGEYSVSDADRERFIGGRFLSTK
                     ILWDMVQPGIDPLSPENVLVVMTSPLTGTGAPCSSRYDISAKSPLTGAIGHSNSGGNF
                     GINLKRAGWDGIVVRGRSKRPVYLDIDGDTVRIKSASKLWGADTQAAQSLMIGKETRA
                     GAMAIGVAGENLVKFASIVSQERSHGRCGMGAVMGSKNLKGMVARGDRKAELAQPEAY
                     KDTIKAWIKMLQNHPATGEMTPRLGTAGFLTALSIRNALPTKNFKRGSYEDAYLIGGE
                     RLADEFLVKNYGCQSCPIRCGRVVELDGKEVKGPEYEILCLLGSNMLINDMPAIIRWN
                     QELDHLGMDAISAGTIMGFAAELNEKGLWKNGIQFGKKDNISKALSDIAHRRGIGKDL
                     AEGVRFLSKKYGGEDFAPHSKGLELAAYEPRASVGHGLGYATASRGACHLDGGYMVYF
                     EVTGPATLDPFHYRSKPAYTVLDQNLVAAISAGGNCLFTSWPILPPILYKLPGHYVLS
                     RFISSILTYSWGLIGLITKLPRGIMRFHMPGLPHSKALNEATGMRMDFGRFYAAGERG
                     YTLERMFNLREGIGRNQDTLSRRFTDEPLLAGNPKSVVRLDKMLPAYYRLRGWDANGV
                     PTSSTLQKLDLAFADERPRTGER"
     gene            6597..9152
                     /locus_tag="PKJ16_11325"
     CDS             6597..9152
                     /locus_tag="PKJ16_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47622.1"
                     /translation="MVMGVSFLGIYAALALSAVYRFIMIVAVAAGLFGVFLFLRSKSK
                     WLELATWLRSSAGAIFLFGCLGSGAIPGLAWIDDQRAALALLVLGIAVNLYLGYAGKT
                     QVFASLHVLLSLIALGIAPQSQTTLICASVIALLSVVLTYRARRDYHLLVTVSGFFFF
                     HLHWYNFSGLVVDGALPFALRVTGIASITIIGISAACVHYRRLYQTTRFDIAPFITHL
                     ANWIFLGASALHYSTGSKWNTIVLAVASVSAFVLARHARRLHIPWLHATDTLISLSLA
                     VFAAFTLQRWGVDALPIAGIIYLEAMIFHVIAVREDTGLLRNISTAIRHLACAGAIVA
                     ALVLMEGADPAGFVKTASIIGALMLFETVFHVYALRSGIARFDSIAAYGTREPETAFG
                     MSGIVIAVLGIAFLGYSHTHAWAPYAMAAFAMLCLVLRQRIQSDSLGVGLLILIPAAH
                     VFSWFHIFGDPALGFSDVLLSAGPYVPVSIAAIALSRIDARDRFLVWPGLYLLVAHAL
                     AIIVVLFKPVSPHIPTIFLLVASAACLEIARFFNERQYTLSTKGGIDRHLLQIGYLLV
                     AIFLVAHLTVNLQLEDYLGPVRVRILIEAFALAVILFWATSPRHAIDYGYKSWRYLHP
                     LMWELSICFSIVVILAEVPPAWLPPVWSVVSIILFAIGRRFESLSRFQFHSTLFHWIA
                     SFWIAFLASTPVPSLRWQDQGWVAGAAAVVLQFGYVIFFYRRAALSDVSFPIGLAPFA
                     RLAERVHRRQNPWLYYPFFASIALFAYWSFDASVRTILWAAESFAIFTVSLIVKENHF
                     RIVSMIGLGACLIRLVAFDLAQSGTLMRAVVFLVVGVIMIAMNALYNKYRDRF"
     gene            9154..>9989
                     /locus_tag="PKJ16_11330"
     CDS             9154..>9989
                     /locus_tag="PKJ16_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV47623.1"
                     /translation="MRPARLIAFALIAASGAALIVVLYRMASAPPIDASFAPLFQLLG
                     RPTKAASSALTRVMPVGDIDEKRFGDAIVERFGGIKQESSKEHRYVNAVMRGLAPFAA
                     KRFEYRVYMMEHPSPNAFALPGGIIFVTTGLLTTLRNESELAAILAHEMGHIELNHCM
                     DSVRFELLARKIEAESLGRLADIAMSLMLRHTFSKTQENEADEYSFELLKNSSYDPYG
                     TAIAFIRLHQASADKPYSHTSPTTSILRDYFSSHPPLQIRANAFSHRAVAWWRSHPQE
                     RR"
BASE COUNT         1806 a         3232 c         2824 g         2127 t
ORIGIN      
        1 tagagatcgc catcgttcct gccgcgccgg aagaagccgg tcaccacgtc cacgacggac
       61 acattgtcgt ccttggcttt tccgatgaca tatccggtaa cgatgagaag cgcgataacg
      121 atcgttttca gtatgcccaa cgtgaacagc agcacgcccg cgagcaatcc ggcggccgca
      181 ccgagggcct tcccgggatt ctcgttgatc caccccccga taaaggcgcg cagatccatg
      241 ttactgcttc acccgctcca cgtactcgcc ggtgcgggta tcgaccttga tcacgtcgtc
      301 ctgattgacg aagatgggaa cattgacggt cgcgcccgtc tccagcttga ccggcttgag
      361 aacgttcgtg gccgtgtcgc cgcgtaaccc gggctcggcg tagacgacct tgagggtcac
      421 gaaggtcggc ggctcgatgg agatcggccg gtcctcgtac atcatgatct cgacctcgtc
      481 gccctccttg atgtagtcga ggatgttccc cacgaacttc ttgtcgatat tcgtcggctc
      541 gtaggtcgcg gtgtccatga acaccagatc gctcccctcc tcgtactggt actgcatcgt
      601 ctggcgatcg atgcgcacgt cctccacctt ctcgccgccg cggaaggtct tgtccaccgt
      661 cgatccgcgc gtgagcgact tgagtttgct gcggacgaac gcgccgcccc gcgccggttt
      721 tacatgctga tattcaatca ccgtatagag gtcgccgtcg atcttgatga ccatcccccg
      781 cttgaagtcg ttgaccgaaa ccattgaacg ctcctcttag atgatcacga tgtccttggt
      841 cgcccgcgtg aggacccggc acccgcccgg cgaaaccacc accatgtcct cgatgcgtac
      901 gccgcccgcg tccggcatgt agatgcccgg ctcgatggtg atgaccatgt ttttcttgag
      961 ccgaatgtcg ccgcgtggcc gcaccgcagg aagctcatgg acctcgagcc ccacgccatg
     1021 tcccagcgaa tgaccgaagc gatcgccaca tccggcgtcg gcgatcacgc ggcgggccgc
     1081 tgcgtcgagc cggccggttg tcatcccggg acggaccgcg tcgattgcgg cttcctgcgc
     1141 acggcggacc gtatcataga tatgccggaa atcgggggca atgcttccca cgaaaaccgt
     1201 tcttgtcaag tctgaattgt agccctgata cacgcatccc atgtcaatca gcaggatatc
     1261 gccctgcgcg atccgcttgg tcatcgaggt ctcgtagtgc ggcatggacg atcccgtccc
     1321 cgaggcgacg atcgaatcga acgatgtctt cgtgcagccg tttttgcggt aaaagtactc
     1381 gatctccacg gcgaggtccc attccagcac gccgggcttc gccagcgtga tgatgtgctc
     1441 gaagcaccgg tccgcgagcg cgaccgcctc ggcgatcacc gcgatctcgc cctcctcctt
     1501 caccatgcgc atctcgttga tctggtcgcc ggcggggacc agcgtggtcc cccgcatgcg
     1561 ttttttcatg acattgaaca gcgacaggga gagatcgtgc tcctcgagga acagccgcgt
     1621 acggcccgct gtcttgagaa tggatttgag cgcggtgcac aggtcgcgct cctgaagaac
     1681 gaagttcacg gattccggaa ggagcttgcg ggcgtattcc tcgtaacgcg aatcggagat
     1741 gaagtagatc gcgtcgcgat caatcaccat tgccgcatac gatccggcga acccggtgag
     1801 atacttcaca ttgaccacgc tggagacgaa atacgggaat ttcttccgtg cgcgaatcgt
     1861 tttttgaatt cgttcgatcc gtgaagtcat gatcgtctcc tttaaccgtg tagcgagagc
     1921 aacacgaccg tgctgcgcgg ctggacgcag tacgatccct gatcggaaag ctcgatctcc
     1981 cggcccggct cgagaaagtc ctcgccggcc ggcttgtcgg tgtcgatgac ccgcaaccag
     2041 cgtgatcccg cgatcgggtc gggaagcctg acggtgttcg ggcggtaatc cgcgttgagg
     2101 acgatgaaca gcgcataatc gcccagcggc gacggctcct cgctgccgtc gagccgatag
     2161 cacagcgtcc gcagctcagg atcatcccag cgcagcggtc cgccatcgac gccgtaccag
     2221 gtgatgtcgg gcatcccgtc gcggtcccaa tcctgccccg tgaggaactt tcgacgctgc
     2281 agtatcgtat atcgcgagcg gaaggcgatc gcctttttca cgaaatccct catgtcggcg
     2341 ttccggcggg tcaggtccca gtcgaaccag ctgatctcat tgtcctggca ataggcgttg
     2401 ttgttcccgt gctgggtgcg caggcactcg tcgccgccca gtatcatcgg gaccccctgg
     2461 gcgagaagga gatagcacac gtggtttttt gcgagcgtgc gacgaagcgc acgaatgccc
     2521 ggatcgtccg tctcgccctc ggcgccgcaa ttccaggagt tgttctcgtt cgaaccatcg
     2581 gcgttgttct ccaggttcgc ctcgttgtgc ttctcgttgt aggagagcag atcgttgagg
     2641 gtgaatccgt catggcaggt gataaagttg attgaattat acgccgtacg tccgtcgtcg
     2701 ccgtacaggt cggccgatcc ggtgatgcgc cagcccatct cacggacctt gccgggatcc
     2761 cccttggcga aggtgcgcac cgtatcgcgg aaacgcccgt tccactccga ccaatcgatg
     2821 ggaaagttcc ccacctgata ggtgcccatg tcccagggtt cggcgatgag cttcacgcgg
     2881 cacagcaccg ggtcctgcgc gacggcgtcg aagaaggacg ccgacgattg gaatgcgccg
     2941 ccctcccggc ccagcaccga tgcgagatcg aaccggaaac cgtcgacatg catcacctcg
     3001 acccagtacc gaagggaatc gaggaccata cgaatgaccg gcgggttcga caggttgagg
     3061 ctgttgccgc agccggaata gttcatgtaa tatcgcgacg ggtgcccgtc gacgccggtc
     3121 aggatatagt acacggtatt gtcgatgccg cgaaaactca tcgtgggacc gagctcgttt
     3181 ccctcggccg tgtgattgta caccacatcg aggatgacct cgatccctgc cgaatggagg
     3241 gcgcggacca gggccttgaa ctcgtcgacc tggcagcccg ggcgcacacc gctcccgaag
     3301 ctcgattcgg gggcgaaaaa gccgatggtg ttatatcccc agtaattcgt gagcccgcgc
     3361 gccacgagaa aatcgtcgac atagctttca tgcaccggaa gcagttccac ggcgttgatc
     3421 cccaagtcct tcaggtacgg gatcttctcg atgaaaccgg cataggtccc cgggtacttg
     3481 accccggacg acgggtgcgc agtgaatccg cggacatgcg tctcgtagat gatcatgcgc
     3541 tcgaagggga tctccggcgg ggtgtcgccc tgccagtcga aatcgtcgga tacgacgatg
     3601 cacttcggca tgatcgacgt gttgtcccga tcgtcgcgcg acaggtcgag tcccggcgca
     3661 ttgggcggat agcacagcag aagattgtcg gcattcacgc actttccggt tagtgcgcgc
     3721 gcgtacggat caagaagaaa gcggttctca ttgtatcgga agccccatga cggattgaac
     3781 tcgccccgca tgcgataccc atagagttgc cccgcggaca ggccgggcac aaacacatgc
     3841 cagatgaatc gcgtgcgatt ttcgagcctg attacatcgg tcggcggcgc ctccgcccga
     3901 tcgaagagca gcagatacac ctcgtgcgcg tgctgggaga atagagcgaa attgacgccg
     3961 ccatcgcgga ttgtggcgcc gagcggatag aaacgccccg gttcaagccg tcgagatgtt
     4021 cggtgcacaa gacccatcat cgttccccgg tgcgcggacg ttcatcggca aatgcgagat
     4081 cgagtttttg cagcgttgac gaggtgggca cgccgttcgc gtcccatccc cgcaatcggt
     4141 agtaggccgg aagcatcttg tccagccgga cgacagattt cgggttacct gcgaggagcg
     4201 gctcgtccgt aaaacggcgg gaaagcgtat cctggttgcg cccgatcccc tcgcgaaggt
     4261 tgaacatgcg ctcgagcgtg taaccgcgct ccccggcggc atagaagcga ccgaagtcca
     4321 tccgcatacc ggtcgcctcg ttcaacgcct tgctgtgcgg caaaccgggc atgtgaaacc
     4381 gcatgatccc gcgcggcagt tttgtgatca gcccgatgag cccccacgaa tacgtgagga
     4441 tcgacgagat gaagcgcgag agcacataat ggcccggaag tttgtacagg atcggcggca
     4501 ggatcggcca gctggtgaac aggcagttcc cgccggcgct gatcgccgcc accagattct
     4561 ggtcgagtac cgtgtaggcc ggcttggacc ggtagtggaa cgggtccagc gtcgccggtc
     4621 cggtaacctc gaaatagacc atatagccgc cgtcgagatg gcaggccccg cggctcgcgg
     4681 tcgcatatcc caggccgtgc ccgaccgatg cgcgcggttc gtacgccgcg agctccagtc
     4741 ccttcgaatg cggcgcgaag tcctcgccgc cgtacttttt cgagagaaag cgaaccccct
     4801 cggcgaggtc cttcccgatt ccgcgccgat gcgcgatatc agagagcgcc ttcgatatat
     4861 tgtccttctt gccgaattgg attccgtttt tccagagccc cttctcgttg agctccgccg
     4921 caaatcccat gatggtcccc gcggagatcg cgtccatccc gaggtggtca agctcctggt
     4981 tccagcggat gatcgccggc atgtcgttga taagcatatt ggagccgagg aggcacagga
     5041 tttcgtactc gggccctttg acctcctttc cgtccagctc caccacgcga ccgcagcgga
     5101 tgggacacga ttggcacccg tagttcttca ccaggaactc gtccgcaaga cgctctccgc
     5161 cgatgagata ggcgtcctcg tagctgcccc gcttgaagtt cttcgtggga agcgcattgc
     5221 ggatcgacaa tgccgtgagg aaacccgcgg tcccgagacg aggcgtcatc tccccggtcg
     5281 cggggtggtt ctggagcatt ttgatccagg ccttgatcgt atccttgtac gcctcgggct
     5341 gtgcgagctc ggccttgcga tcgccgcgcg cgaccatgcc cttgaggttt ttcgagccca
     5401 tgaccgcgcc catgccgcag cggccgtggc tgcgttcctg cgacacgatc gaggcgaact
     5461 tcaccagatt ctcccccgcc acgccgatgg ccatcgcgcc cgcgcgtgtt tcttttccga
     5521 tcatgaggga ctgcgccgcc tgcgtgtccg cgccccagag cttcgatgcg ctcttgatgc
     5581 gcaccgtgtc gccgtcaata tccagataca ccggccgttt cgaacgcccg cgcaccacga
     5641 tgccgtccca ccccgcgcgc ttgaggttga tcccgaaatt gccgccgctg ttcgaatggc
     5701 cgatcgcacc ggtgagcggg ctcttcgcgg aaatgtcgta ccggctcgag cacggggccc
     5761 cggtcccggt caggggcgat gtcatcacga caagtacatt ctccggcgag agcggatcga
     5821 tccccggttg caccatgtcc cagaggatct tggtgctcag gaaacgcccg ccgatgaaac
     5881 gctcgcgatc ggcatcgctc accgaatatt cgccgatcct gccgttcgac aaatcgacgt
     5941 ccataatcct gcccatgtat cccttgaaag cgctcgccat gtgcattcac ctcgttttcg
     6001 cctgatagaa ccgatgcaat ccaaatcgac agccacgtta cacgacgagt ctatagtacc
     6061 ggaccacatt ttcatttgtc atcaatattt ttcaatcatc ccgtcgcacg gcagttagcg
     6121 tccgcgcgcc atggtattct gcttgacgca catgacctga cgtggtggag tatggcgacg
     6181 gacgcacacc gcgaattgaa atggaatctt tcattatttt catactgtgc attcttatga
     6241 tcgcgctgct cgtccggcag cgccggctgt ctcttcgtat cggcgccatc gaaaagcgta
     6301 tcgcagaagg tgccgggacc caaaccgaac gacccatcac cccggcacgc gttcctttgc
     6361 ccaccgcaca ccctgaaccc gttgctatat ccggttctcc tgcaaccgac atccattcgc
     6421 gttctacgtc accatcgact gaccaccctg cggaaatctc gacgaaagcg gcgccatcgt
     6481 cgccagttac cccgagtccc tcggccgaac cggcttcgcc agctgaaccg acgctactta
     6541 aacgcatcga aacgcagttc gcccaaaact ggacgggcat acttggcgcc atcatcatgg
     6601 tgatgggggt gagtttcctc gggatctatg cggcattggc gctcagcgcc gtctatcgct
     6661 tcatcatgat agtcgccgtc gctgcggggt tgttcggcgt attcctgttc ctgcgctcga
     6721 aatcgaagtg gctggagctc gccacctggc tccgcagcag cgccggcgcc atcttcctct
     6781 tcggctgcct cggatcgggc gccattcccg gcctcgcatg gatcgacgat cagcgcgccg
     6841 cgctcgcgct gctcgtgctg ggcattgccg tgaacctgta cctcggctat gccggaaaaa
     6901 cgcaggtatt cgcctcgctc cacgttcttc tcagtctgat cgcgctgggg atcgcgccgc
     6961 aatcgcaaac gaccctcatc tgcgcatcgg tcatcgcatt gctcagcgtc gtgctcacat
     7021 accgcgcgcg gcgcgattat cacctgctag tcacggtctc cgggttcttc tttttccatc
     7081 tgcactggta taatttttcg ggactcgttg tcgatggcgc gctcccgttc gcgcttcgcg
     7141 tgacgggcat cgcctcgatc accatcatcg ggattagcgc ggcctgcgtg cactatcgcc
     7201 ggctctacca aacgacacgg ttcgatatcg cgccctttat cacccatctc gcgaactgga
     7261 ttttccttgg agcaagcgcc cttcattatt ccaccggctc caagtggaac accatcgtgc
     7321 tcgccgtcgc gagcgtctcg gcgtttgtgc tggcacgcca tgcgcgccgc ctccatatcc
     7381 cctggctgca cgcgaccgac accctcatct cgctctcgct ggccgtcttc gccgcattta
     7441 cgctccagcg ctggggagtg gacgcgctcc caatcgctgg catcatctac ctcgaagcga
     7501 tgatttttca cgtgatcgcg gtgcgcgagg acaccggcct gttgcgaaat atctccaccg
     7561 ccatccgcca cctcgcctgc gccggggcca tcgtcgcggc attggtcctc atggagggcg
     7621 ccgacccggc cgggttcgtc aagaccgcga gcatcatcgg cgcgttgatg cttttcgaga
     7681 cggtgtttca tgtctacgcc cttcgctcgg gaatcgcgcg gttcgattca atcgccgcct
     7741 acggcactcg cgaaccggaa accgctttcg gcatgagcgg aatcgtcatc gccgtgctcg
     7801 ggatcgcctt cctgggatat tcgcatacgc atgcatgggc cccatacgca atggcggcat
     7861 tcgcgatgtt gtgtctcgtc ctccgccagc ggatccaatc cgactcactg ggggtcgggc
     7921 ttctcatcct gattccggcc gcacatgttt tctcgtggtt tcatatcttt ggcgatcccg
     7981 cactcgggtt ttccgatgtc cttctttcgg ccggaccgta tgttccggta tcgattgccg
     8041 ccatcgcgct ttcacgcatc gatgcgcgcg accgcttcct cgtctggccg ggactttacc
     8101 tgcttgttgc gcacgcgctg gcgatcatcg tggtactctt caagcctgtc tctccgcata
     8161 tccccacgat cttccttctc gtggcatcag ccgcctgcct tgaaatcgcg cggtttttca
     8221 acgaacgtca atatacgctt tccacgaagg gcggcatcga tcggcatctg ctgcagatcg
     8281 gctacctgct tgtcgcgatt tttctggtcg cccatctgac ggtcaacctg cagctcgaag
     8341 attacctcgg accggtgcgt gtgcgcatcc tcatcgaggc gtttgcgctt gccgtgatcc
     8401 tgttctgggc gacatcgcca cgacacgcaa tcgactacgg ctacaagagc tggcgctatc
     8461 ttcatccgct catgtgggag ctgtcgatat gtttctccat cgttgtcatt cttgccgagg
     8521 tcccgccggc atggctgcct ccggtctgga gcgtcgtttc aatcatcctg ttcgccattg
     8581 gacgaaggtt cgagtcgctg tcccgcttcc agtttcattc gaccctattc cactggatcg
     8641 cttcgttctg gatcgccttt ttggcgagca cgccagtccc ctcactccgg tggcaggacc
     8701 agggctgggt cgccggcgca gcggcggtgg tgcttcagtt cgggtatgtc atcttctttt
     8761 accgaagagc cgccctttcg gacgtatcat ttccgatcgg cctcgcgccc ttcgcgcggc
     8821 tcgccgagcg cgtacatcgg cgccaaaacc cgtggctcta ctacccgttt ttcgcctcga
     8881 tcgccctctt cgcctactgg tccttcgatg cctccgtccg gactatcctc tgggcggcgg
     8941 aatcgttcgc gatcttcacc gtaagcctca tcgtgaagga aaaccacttc cgcatcgtct
     9001 cgatgatcgg tctcggggct tgcctgatac gattggtcgc cttcgacctc gcgcaatcgg
     9061 gcacgctcat gcgggccgtg gtcttcctcg tggttggcgt catcatgatc gcgatgaacg
     9121 ccctgtacaa taaataccgg gacaggttct aacatgcgtc cagcccgatt gatcgcattc
     9181 gcgctcattg cggcctccgg ggccgcgctg attgtcgtcc tctatcgcat ggcgagcgcg
     9241 ccgcccatcg atgcgagctt cgccccgctg tttcagctcc tggggcggcc caccaaggcg
     9301 gccagcagcg ccctcacccg cgtcatgccg gtcggcgata tcgacgagaa gcggttcgga
     9361 gacgcaatcg tggagcgctt tggagggatc aaacaggaga gttcgaaaga gcaccggtat
     9421 gtaaacgccg tcatgcgggg actcgctcca tttgccgcaa aacgcttcga ataccgcgta
     9481 tatatgatgg agcacccatc acccaacgcc tttgcccttc ccggcgggat catttttgtc
     9541 acgaccggcc ttctcacgac cctgcggaac gagtccgagc tcgcggccat cctggcccac
     9601 gagatgggcc atatcgagct caaccactgc atggacagcg tccgtttcga gcttctcgcg
     9661 aggaagatcg aggccgaatc gctcggcagg ctcgccgata tcgccatgtc cctgatgctg
     9721 cgccacacct tcagcaaaac ccaggaaaac gaggccgacg aatactcgtt cgaactgctg
     9781 aagaactcgt cctacgatcc gtacggcaca gccatcgcat ttattcgtct gcatcaggcg
     9841 tccgccgaca aaccatactc ccacacatcg cccacgacga gcatcctgcg cgactatttc
     9901 agctcgcacc cgccgcttca gattcgggcg aacgccttct cccatcgggc cgtcgcatgg
     9961 tggcgctccc atccgcagga gcgccgata
//
LOCUS       DAORHF010000129         9973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6097_length_9973_cov_5.578645, whole genome shotgun sequence.
ACCESSION   DAORHF010000129 DAORHF010000000
VERSION     DAORHF010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9973
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6097_length_9973_cov_5.578645"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="PKJ16_11335"
     CDS             <1..949
                     /locus_tag="PKJ16_11335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HNV47624.1"
                     /translation="YNLLRTRFYARTATDTGAQARITIEKKHEGWPGIPHGGMGMTAL
                     IELTDLIDGTMDRYPLDTRFRFGGERLALGDTVDIGIEKREDAFHGAIRTPGGVLPYL
                     SATIKNAVNPAHEERVRKLSERLAVNALSHNSFLMPDFSDRLIFRQTFQDLHRYRLYE
                     FRDQPDGSVIMICFFSDRAGGLPANELNTITDDHVHPGPLITLLDETLGWAGFLAVWQ
                     GGVTVTLDAYFMRPLRPDEIIFAVGICDSQQGSFTRKMVTCSGGIFSRTGDSIEPVAY
                     ATGKWLTKPEFKEKMLAYLLHDDNYLSAAAIYNKSARER"
     gene            complement(950..2215)
                     /locus_tag="PKJ16_11340"
     CDS             complement(950..2215)
                     /locus_tag="PKJ16_11340"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HNV47625.1"
                     /translation="MNNSKNILLGALIGNALGCPLDGLRKGHIGATLKNIHGYVDPLP
                     ALKGKEHRWKKPGLYDSIGQLMLLMALSNSPRRGFARDSFLQLVRSGMDLVEGGQGIF
                     RHPGPMELHLMSRIAAMPEGPPHPIASARLSAIAAPALAIGARSRDELFISAISAGTL
                     FTSDSHSLGGAALCALTLYAQLEDDAAGEDAMDIAAREGRWLIDAAARHSADLFDAKL
                     NPDSFAASCETIASLFAELAGVDDPAAAEHAICARADHANPATRATVDHPLYALPYAM
                     FVAGRGTGGDGERLIRLMREGGATSAMCAIAGACTGARTRDDAFLDDLTDGLVNRKRV
                     LALLDRLALGRGAPGMDDDFLLSEKALTLKEREELSARTRHAPVKERKTKRPKDGVTE
                     LSRHVVESWTKIDKARWQRQKKRLPDPGG"
     gene            complement(2635..4107)
                     /locus_tag="PKJ16_11345"
     CDS             complement(2635..4107)
                     /locus_tag="PKJ16_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV47626.1"
                     /translation="MTTTKTEYNLATMILQAAEEKPDGVFLEYRGNPITFREGLDRSR
                     RCAGFLRSGGVEQGDRVLIILGNIPEFIYSYLAIVQLGAIGVLVNPAARRYEFRHYIE
                     ETEPKYIITDSKQIEHFKTDEGFFFDPDRVICVDADAAHNRFGDILERSQPYEGIAPV
                     EEYATASIIFTSAMEGKPLGAQITHAGIFGTARAYAVNPVLHTDKFLLTLPLFHSYGL
                     ASSLFTPLYKKIPTLLVNRYSPKRIHEILLNDGVTIFCGVPIMFQLIPFAAPEGTRYP
                     SLRVWVSGGEAISVELQKLLMDRFEIDIRQGYGLTEASPIVTWNLIGTPNKFGSIGIP
                     MVWNEIKIERPDGSIAKPNEEGELIVKGVNVISGYYKNPEKTREVIRDGWLHTGDLGY
                     VDADGYFYITGRKKDMIIKNGLNIYPKEVERLISMHPSVENVRITGHVVKNEDSTTKE
                     TITAEIYRKKGHTVDKESLIEWCKGNISSYKLPDDFDIRN"
     gene            4336..4782
                     /locus_tag="PKJ16_11350"
     CDS             4336..4782
                     /locus_tag="PKJ16_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47627.1"
                     /translation="MVVLVFILILQVSFAIHIFYMVSYISRKENKDFKGFIVTAVTNI
                     FLGIFVALFILINPEELREINLDRVLLIESGVIFFAMVYIKGKVASRIYRLSQDPRYY
                     HYSYFGKKVLDKSVVTNRDLFLFFLSIPLTLICGAYFVMRLCCAGL"
     gene            complement(4733..5908)
                     /locus_tag="PKJ16_11355"
     CDS             complement(4733..5908)
                     /locus_tag="PKJ16_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HNV47628.1"
                     /translation="MYPINSDRLVRTFVDLASISSPSWKEEGVIAYLTDALGAMGVSC
                     ERHPCGDSFNLLASVSGAVKRPSILLSAHMDTVVPCDNVRPVVRGGKIVSDGTTILGA
                     DDKAAIAAFLEALASIRERDMKHGPVELLFSCAEEVGLSGIKGFDARVLRSRLGFVFD
                     SSGPVGAIVVAAPYHLSFRLVVKGRAAHAGIEPEKGASAIRALGEIMATIPHGRIDAE
                     TTANIGTIAGGRATNIVADEAICALEIRSRSKTKLAAVEARMRAVVDTVAHRHGVKVR
                     VHRSLEYSGFSYRPSDPVVAAARRAIERIGLVPALEASGGGSDTNVLNRAGIPSVALS
                     VGMQKVHTTGECIRIRDLENAARLAMALIDPEQADPAGRSRATTPRNRAASRNRRRR"
     gene            complement(5941..6462)
                     /locus_tag="PKJ16_11360"
     CDS             complement(5941..6462)
                     /locus_tag="PKJ16_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47629.1"
                     /translation="MIHSHVTTRSALRAMCAVLALALGFARCTDVKEYYLDPAKPPLG
                     IKILVLDDPGYAGDYLLVFSSENRGNTRFGGFLVFIAETRDGVMAIEDHNLAKFRFEG
                     SDYNLGIDKPVAILFSNGAMAQELAGYSVVALRPKADMAPVGSWLTMRSYLIDDNKNV
                     IELSSPGNPVQIP"
     gene            complement(6459..6974)
                     /locus_tag="PKJ16_11365"
     CDS             complement(6459..6974)
                     /locus_tag="PKJ16_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47630.1"
                     /translation="MLKRYLTCAMALAVLATGARCAMDVPPRGYDYLTPPIITSLEQR
                     TVQDGSDTILEIEVRFIGYNNEYYFDGYNVYVSPNSMNRDLIESYRPVPIGEHGYAMA
                     SPSYPLGPDDYDPKVERRMTLRYYAMANDDVYTLYTFGPYTYYVMLCSHHREGLVYPE
                     SVSNQVAILLQ"
     gene            complement(6985..7509)
                     /gene="lepB"
                     /locus_tag="PKJ16_11370"
     CDS             complement(6985..7509)
                     /gene="lepB"
                     /locus_tag="PKJ16_11370"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HNV47631.1"
                     /translation="MFGRDRDDTIKTPLFIAAACVLAGLVAGVALVRLVITPYAVSDD
                     SMRPGLAAGDRIYVLKWGAVRWGDRVLFASPIEPDRVLLKRVIGVEGDTIEVSGKAIY
                     RNGAKLSAPWKTGSTDRRIFPMSFTGRDNSPTVRIGRGELYVVGDNLDRSMDSRTFGP
                     VRMKSVIGTVLGRR"
     gene            7658..8863
                     /locus_tag="PKJ16_11375"
     CDS             7658..8863
                     /locus_tag="PKJ16_11375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV47632.1"
                     /translation="MADLPVGEKIYGALEKSSWIRRMFEVGTALKKEHGEENVFDLTL
                     GNPDLDPPLEFFDTIAALIQERKKGAHGYMPNAGYPDVLDTVGRLVSATHGVEVGGRS
                     IVMTCGAAGGLNVVFKTILDPGDEVIVPRPYFAEYGAYIDNHGGRMVTVDTAADFSLD
                     VDAIDAAMTARTRAVLINTPNNPTGKLYREAELAALGALIDARAGTTRPVYLIADEPY
                     REIVYDGLEAPPILKQCRQSIVVTSYSKSLSIPGERIGYIAVNPSCAGADTLMAGLIM
                     CNRILGFVNAPALMQRAVAALATARVNTQAYTKRRDLICDGLEYAGYRFDRPEGAFYV
                     FCKSPIPDDVAFVQHLQRYNILTVPGVGFGGPGHFRICYCVPESVITRAIPRFKEALD
                     TLPKGTANS"
     gene            9089..9271
                     /locus_tag="PKJ16_11380"
     CDS             9089..9271
                     /locus_tag="PKJ16_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47633.1"
                     /translation="MADETNAAQTDGAASQKTKKINRLPKAGIEKKIAELEEKGQTRS
                     VYYKHLVARRSELNPS"
     gene            complement(9282..9590)
                     /locus_tag="PKJ16_11385"
     CDS             complement(9282..9590)
                     /locus_tag="PKJ16_11385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47634.1"
                     /translation="MNVTFSHEDDWFRIKLAGEIDLYNVYTIKDQVKEKLKESPSNLS
                     VDVSEINYLDSSGIGMLVELKRMVEETGKIYRLRNIPPNILQLFKAINLDKILKDNIE
                     "
     gene            9736..>9973
                     /locus_tag="PKJ16_11390"
     CDS             9736..>9973
                     /locus_tag="PKJ16_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HNV47635.1"
                     /translation="MKPTTISFLVSGRGSNFQAVAAAVRDGSVPATLGIVISDRAGAG
                     ALDIARQFGMGAFHVDAKAYPGKREYESAMVELLE"
BASE COUNT         2030 a         3067 c         2885 g         1991 t
ORIGIN      
        1 gtataacctt ttgcggacac gcttctatgc acgcaccgca acggacacag gcgcgcaggc
       61 gcgcatcacc atcgagaaga agcacgaggg gtggccgggc ataccccacg gggggatggg
      121 gatgacggcg cttatcgagc ttaccgacct catcgacggg accatggacc gatacccgct
      181 ggacacccgc tttcgcttcg gcggcgagcg actggcgctc ggggacaccg tcgacatcgg
      241 cattgaaaaa cgggaggatg ccttccatgg cgcgatccgc acaccgggcg gcgtcctgcc
      301 ctatctttcc gcgacaatta aaaacgcggt taaccccgcg cacgaggagc gcgtccggaa
      361 actgagcgaa cggctcgccg tgaacgccct gagccacaat tcatttctca tgcccgattt
      421 ctccgaccgg ctcatcttcc gtcagacgtt tcaggacctc caccggtatc gactgtacga
      481 gttcagggac caacccgacg gatcggtgat catgatctgc tttttcagcg accgggcggg
      541 ggggctgccg gcgaacgagc tcaacaccat cacggacgat cacgtgcatc cgggcccgct
      601 catcaccctg ctcgacgaga cgctcggctg ggcgggcttt ctggcggtgt ggcagggcgg
      661 cgtaacggtc accctggatg cgtatttcat gcggccgctg cgtccggacg agatcatctt
      721 tgcggtgggg atatgcgata gccagcaggg ctcgttcacc aggaagatgg tgacctgctc
      781 cggcgggatc ttttcccgca cgggagattc gatcgagccg gtcgcctacg ccaccgggaa
      841 gtggcttacc aagcccgagt tcaaggagaa gatgctcgcc tatctgctgc acgacgacaa
      901 ttacctttcg gccgccgcca tctacaacaa atcggcgcgc gagcgctgat caaccgccgg
      961 gatcggggag acgcttcttc tggcgttgcc agcgcgcctt gtcgatcttg gtccagcttt
     1021 ccaccacgtg ccgcgaaagt tcggtcacgc cgtccttcgg ccgtttcgtc ttccgctctt
     1081 tgaccggcgc atggcgggtg cgcgcagaaa gctcttcgcg ctccttgagg gtgagcgcct
     1141 tttcggacag aagaaaatca tcgtccatgc ccggagcgcc ccgtccaagc gcgaggcgat
     1201 ccagcagggc gagcacccgc ttgcggttta ccagaccgtc cgtcaaatca tcaagaaacg
     1261 cgtcgtcacg ggtgcgagcg ccagtgcagg cccccgcgat cgcgcacatc gccgatgtcg
     1321 ccccgccctc gcgcatcagg cgaatcaagc gctctccgtc gcccccggtg ccgcggccgg
     1381 caacgaacat cgcgtagggc aatgcgtaca gcggatggtc gaccgtggcg cgcgttgcgg
     1441 gattcgcatg atccgcacgc gcgcagatgg cgtgttccgc cgcggcggga tcgtccacgc
     1501 ccgccagttc ggcaaagagc gacgcgatcg tttcgcacga tgccgcgaag gaatcgggat
     1561 tgagcttcgc gtcgaaaagg tccgccgagt ggcgcgccgc cgcgtcgata agccagcgac
     1621 cctcacgcgc ggcgatgtcc atcgcatcct cgccggcggc gtcgtcctct aactgcgcat
     1681 agagcgtcag cgcgcacaga gcggcgcctc cgagcgagtg ggaatcgctc gtgaacaatg
     1741 tccccgcgga tatcgccgag atgaagagct catcgcgcga tcgggcgccg atagccagcg
     1801 ccggcgcagc gatcgccgac aggcgcgcgc tcgcaatcgg atgcggcggg ccctccggca
     1861 tggcggcaat acgcgacatg aggtgcagct ccattgggcc cggatggcga aaaataccct
     1921 gccccccctc gaccaggtcc attccactgc gaaccagttg cagaaaggaa tcccgtgcga
     1981 atccccgccg cggagaattg ctcagggcca tcagtagcat caactgcccg atcgaatcgt
     2041 acagtcccgg ctttttccag cggtgctcct tgcctttgag cgctggaagc ggatcaacat
     2101 atccgtgaat attctttaac gtggccccga tgtgcccctt gcgcaagccg tccaggggac
     2161 aacccaatgc attcccgatc agagcgccaa gcaggatatt ttttgaatta ttcaaattga
     2221 aaacaggtat gtccgtattt caaaacaggt gtccattgaa acatacactc tggatgatta
     2281 ttttgcacat tttaaatatt ttcgcttagt taaacacacc aattgtggtt caagcaatta
     2341 tcatcttgcg cagtttaatc gctccctctg aactgtgaat cttaaaatac actatgtagc
     2401 ggcataatag aaaaattcca atgcaaatac ttgttattct gctgaaattt tttgatgaaa
     2461 aaaaaataat accaaagaca gagcatttgg cacgatactt gcaatataat atgggtgagc
     2521 tgggataatc ctggtacact taatttcctt ccaaaatatt ccgctaaatg taagaggtat
     2581 tctctcgaat acctctcttt ttatatatgg ctacattttt tacaaattgt tcagttaatt
     2641 gcgaatatca aagtcatccg gaagtttata tgaagaaata ttacccttgc accattcgat
     2701 cagcgattcc ttatcgacag tatggccttt ttttcgataa atctcggcgg taatggtttc
     2761 ttttgttgtg gaatcttcgt tcttgacgac atgtccagtt atccggacat tttccactga
     2821 gggatgcatt gaaatcagcc gttcaacttc tttggggtat atattaagtc cattcttgat
     2881 gatcatatcc ttttttcgcc cggttatata gaaataacca tccgcatcga cataacccaa
     2941 gtctcccgtg tgcagccatc catcccgaat cacctcgcgg gtcttttcgg ggttcttata
     3001 atagccggag atgacattta cgcccttgac aatgagctcg ccctcctcgt tcggcttcgc
     3061 gatggagccg tcggggcgct cgatcttgat ctcgttccag accatgggta tgccgattga
     3121 gccgaacttg ttcggcgttc caatcaggtt ccaggtgacg atgggcgagg cctccgtgag
     3181 cccgtacccc tgccggatgt cgatctcgaa gcggtccatc agcagctttt gcagctcaac
     3241 cgatatggcc tccccgcccg acacccatac ccgcaggctt ggatagcgcg tcccctccgg
     3301 cgccgcgaag gggatgagct ggaacatgat cggaacaccg caaaagatgg tcacgccgtc
     3361 attgagcagg atctcgtgaa ttcgcttcgg cgaataacga ttcaccagca gcgtggggat
     3421 cttcttgtag agcggcgtaa agagcgacga tgccagcccg tacgaatgga agagcggcag
     3481 cgtgagaaga aacttgtcgg tatgcagcac cgggttcacg gcataggccc gggcggtgcc
     3541 gaagataccg gcatgggtga tctgcgcgcc cagcggtttt ccctccatgg ccgaggtgaa
     3601 gatgatcgag gcggtggcgt actcctccac cggcgcaatt ccctcgtagg gctgcgagcg
     3661 ctcgaggatg tcgccgaagc ggttgtgcgc cgcatcggca tcgacacaga tgaccctgtc
     3721 cggatcaaag aagaacccct cgtcggtttt aaagtgttct atctgcttcg aatcggtgat
     3781 gatgtatttc ggctccgttt cctcgatata gtggcggaac tcgtaccggc gcgcggccgg
     3841 gttcaccagc acgccgatgg cccccagttg gacaatggcg aggtaggaat aaatgaactc
     3901 ggggatgttg cccaggatga tgagcacccg atcaccctgt tcgacgccgc cggaacgaag
     3961 gaaccccgca cagcgccgcg atcggtccag gccctcccgg aaggtgatgg gattcccgcg
     4021 gtactcaaga aagacgccgt ccggcttctc ctcggccgcc tgcaggatca tcgtcgcgag
     4081 attgtactcg gttttggttg tggtcataca tcccctcaat aaatgcgatc ggtgataaaa
     4141 aaagcggagt ttatggcgct ttttaattaa ccgtcaattg ttttttacac ccattcaaat
     4201 ggtcccacac cgggcaggag tatgccgggc ggcgcgcttt tacttgacgg attcgccgat
     4261 caggggatat gcttgcgccg ttcattgcgt gcgccgtcac aacgtttcgg aatccggacg
     4321 gaatatcacc catccatggt tgtcctcgta tttatcctga tcctgcaggt gtccttcgcg
     4381 atacatattt tctatatggt gagctacatc tcgcgcaagg agaacaagga cttcaaggga
     4441 ttcatcgtga ccgcggtcac caatattttt ctgggcatct tcgtcgcgct gttcattctc
     4501 atcaatcccg aggaactgcg cgagatcaac ctcgaccgcg tgttgctcat cgaatcgggg
     4561 gtgatctttt tcgccatggt gtacatcaag ggaaaggtcg cctcgcggat ctaccgcctc
     4621 tcacaagacc cgcggtacta tcattattcg tattttggaa agaaggtgct cgataaatcc
     4681 gtcgtcacca accgcgacct gtttctcttt ttcctcagca tcccgctcac gctcatctgc
     4741 ggcgcctatt tcgtgatgcg gctctgttgc gcggggttgt agcgcgcgaa cggcccgctg
     4801 ggtccgcctg ttccgggtca atgagcgcca tcgcgagccg cgcggcgttc tccagatcgc
     4861 gaatacggat acactcgccc gtggtgtgca ccttctgcat ccccaccgag agcgcgaccg
     4921 acggtatccc cgcgcgattg agcacattgg tgtcgctccc gccccccgag gcctcgagcg
     4981 cgggaacaag gccgatccgt tcgatggccc gtcgtgcggc ggcgaccacc gggtccgagg
     5041 gccggtaaga gaaccccgag tattcgagcg atcggtgaac ccgcaccttg acgccgtggc
     5101 gatgcgcgac tgtgtcgacg acggcgcgca tccgggcctc aacggcggcg agcttcgtct
     5161 tcgagcgcga gcgaatttca agcgcgcaga tcgcctcgtc ggcgacgatg ttcgtggcgc
     5221 gccctccggc gatggtgccg atgttcgcgg tcgtctcggc gtcgatgcgc ccgtgcggga
     5281 tcgtggccat gatctccccg agcgcccgga tcgcgctcgc gcccttctcc ggctcgatgc
     5341 ccgcatgcgc cgcgcgcccc ttcaccacga gtcgaaaact gaggtgatac ggcgccgcca
     5401 cgacgatggc cccgaccgga ccgctgctgt cgaacacgaa gccgagccgc gagcgcagca
     5461 cgcgcgcgtc aaagcccttg ataccggata gccccacctc ctcggcgcag gagaacagca
     5521 gctcaacggg tccgtgcttc atatctcgct cgcggatgga ggccagggcc tccaggaagg
     5581 ccgcaatggc ggccttgtcg tccgcgccca ggatggtggt cccgtccgag acgatcttcc
     5641 cgccgcgcac aaccggccgc acattgtcgc agggaacgac cgtgtccata tgggccgaaa
     5701 ggagaatcga cggccgcttc acggcacccg agaccgaagc gaggagattg aacgaatccc
     5761 cgcaagggtg ccgttcacac gacacgccca tcgcgccaag ggcgtcggtc agataggcga
     5821 taacgccctc ctctttccat gacggggagg atatcgacgc aagatcgacg aaggtgcgca
     5881 ccagacgatc ggaatttatc ggatacatgt cactcctcga agcgcaggat ggcgcgggca
     5941 tcagggtatc tgcaccggat tgcccggcga gctcagctca atgacattct tgttgtcgtc
     6001 aatcaggtag gagcgcatgg tgagccagct cccgaccggg gccatgtcgg ccttcgggcg
     6061 aagcgccacc acgctgtacc cggcaagctc ctgcgccatc gccccattcg agaacaagat
     6121 cgcgaccggc ttgtcgatgc cgagattgta atcgctcccc tcgaaacgga acttcgccag
     6181 gttgtggtcc tcgatcgcca tgacgccgtc gcgggtctcg gcgatgaaca ccagaaagcc
     6241 gccaaaccgc gtgttccccc ggttctccga cgagaagaca agaagatagt ctcccgcgta
     6301 gccgggatcg tcaagcacga gaattttgat tccgagcggc ggtttcgcgg gatcgagata
     6361 gtattccttc acgtcggtgc agcgcgcaaa gccgagggcc agcgcgagaa ccgcgcacat
     6421 cgcccgaagg gcactacggg ttgtgacgtg cgaatgtatc attgcagaag gatcgccacc
     6481 tgattactca cgctctccgg atataccagg ccctcccgat ggtgcgagca cagcatcaca
     6541 taataggtgt acggcccaaa ggtgtagagc gtatacacgt cgtcgttggc catggcgtaa
     6601 tagcgcaggg tcatgcgacg ctcgaccttt ggatcataat cgtccggccc caacggatac
     6661 gacggtgacg ccatggcgta cccgtgctcg ccgatgggta ccggccgata gctttcaatg
     6721 aggtcgcggt tcatgctgtt gggcgaaaca tagacgttat agccgtcgaa gtagtactcg
     6781 ttgttgtagc cgatgaagcg cacctcgatc tcgagtatgg tatcgctgcc atcctggacg
     6841 gtgcgctgtt ccaacgaggt gatgatgggc ggcgtgaggt aatcgtagcc ccggggcggc
     6901 acgtccatgg cgcaccgcgc gcccgtcgcg agcacggcaa gcgccattgc gcatgtcaga
     6961 tatcgtttca gcatcgccat cgcgttagcg gcgtcccagc accgtcccaa tgaccgactt
     7021 catccggacc ggcccaaagg tccggctgtc catgctgcgg tccaggttgt cgccgacgac
     7081 gtacagctcg ccgcgcccaa tcctgacggt cgggctattg tcgcgccctg taaaactcat
     7141 cggaaatatc cgacggtcgg ttgaccccgt tttccacggc gcgctcaact tcgcgccgtt
     7201 gcggtatatc gccttgccgg acacctcgat ggtgtcgccc tcgacgccaa ttacgcgttt
     7261 caggagcacc cggtcgggtt caatgggact cgcaaagagc acgcggtccc cccatcgcac
     7321 cgcaccccac ttgagaacgt agatccggtc gcccgcggca aggcccggcc gcatggagtc
     7381 gtcggaaacg gcgtaggggg tgatgacgag ccgcaccagc gcaacccccg ccacgaggcc
     7441 ggccaggacg caggccgccg caatgaaaag cggcgttttg atggtatcgt cgcgatctcg
     7501 tccaaacatg gtgtcgattc ccgcccgccg ggcgcgatat tgtcaagaag aatacccgcg
     7561 ctccggccgt cggcagcggg cgttgaaaaa attgcttgta aaatgccgct cgcgaacata
     7621 gcgataccca tcatcataac cctacaggac ggcaccaatg gcggatttac cggtcgggga
     7681 aaagatctac ggcgcactcg agaagtcgtc gtggatacgg cggatgttcg aggtgggcac
     7741 cgcgctcaag aaggaacacg gcgaggagaa cgtgttcgac ctcacgctcg gcaatcccga
     7801 tctcgacccg ccgctcgaat ttttcgacac gatcgccgcc ttgatacagg agcggaaaaa
     7861 gggcgcgcac ggctacatgc ccaatgccgg ctacccggac gtgctcgata ccgtcgggcg
     7921 cctcgtctcc gccacgcacg gcgtcgaggt gggcggtcga tcgatcgtca tgacctgcgg
     7981 cgcggccggc ggcctcaatg tggtcttcaa gacgatcctt gacccgggcg acgaggtcat
     8041 cgtcccccgg ccgtacttcg ccgagtacgg cgcctacatc gacaaccacg gcggccgcat
     8101 ggtcacggtg gacaccgctg cagatttctc gttggacgtg gacgccatcg acgcggcgat
     8161 gaccgcacgg acccgtgcgg tcctgatcaa caccccgaac aaccccacgg gcaagctcta
     8221 ccgcgaggcc gagcttgcgg ccctcggcgc cctcatcgat gcgcgcgcgg gcaccacgcg
     8281 ccccgtgtat ctcatcgccg acgagccgta tcgggagatc gtctacgacg gcctggaggc
     8341 gccccccatc ctgaagcagt gccggcaatc gatcgtggtc acctcttatt cgaagtcgct
     8401 gtcgatcccc ggcgagcgca tcggctacat cgccgtcaat ccctcctgcg ctggcgccga
     8461 tacgctgatg gcggggctga tcatgtgcaa ccggatcctc ggcttcgtca acgcgccggc
     8521 cctgatgcag cgtgcggtcg ccgcgctggc gacggcgcgc gtgaacaccc aggcctacac
     8581 gaagcggcgc gacctcatct gcgacggcct cgaatacgcc gggtaccgct tcgaccgccc
     8641 cgagggcgcg ttctacgtgt tctgcaaatc gcccatcccc gacgacgtgg cctttgtcca
     8701 gcacctccag cgctataata tcctgacggt ccccggcgtg ggatttggcg gaccgggcca
     8761 tttccggatc tgctattgcg tccccgaaag cgttatcaca cgggcgatcc cccgcttcaa
     8821 ggaggcgctg gacacgctcc cgaagggaac agcgaatagc taagtaactg ctgctgcgga
     8881 cgtactgcat gcggatgcag acgccaatac caagagcatt gttgacaata cacgccgcag
     8941 ggcatcacgc ccaatcagcg acagcgacgg gagacggtcc taatagtaat tgacaggacc
     9001 agccggcgcc tcgcacactg cgattcgacc gacggtgatc cggcatcgcg ccgtgatgag
     9061 ccgcggatta ttcgatacga ggtattccat ggccgatgaa accaacgccg cacaaaccga
     9121 tggcgccgcc agccagaaga cgaagaagat caaccggctg cccaaggcgg ggatagagaa
     9181 aaagatcgcc gagctggagg agaagggcca gacgcgatcg gtgtactaca agcaccttgt
     9241 cgcgcgccgg agcgagctta atccctcgta gaccgacccg actattcgat attgtccttg
     9301 aggattttgt ccaggttgat cgccttgaac agctggagta tgttcggcgg gatattgcgc
     9361 agacgatata ttttcccggt ctcctcgacc atccgcttga gctcgaccag catgccgatt
     9421 cccgaggagt cgaggtagtt tatctcggag acatcgaccg agagattcga gggagactcc
     9481 ttgagtttct ccttaacctg gtccttgatc gtgtagacat tgtacaggtc gatctcgccg
     9541 gccagtttga tccggaacca gtcgtcttca tgtgaaaagg taacattcac cgatagttct
     9601 ccgcggcgcc ggctgagcgg acacggcacc gtagatatag tcccgtccag tcatcgaaat
     9661 gtcaatcgta acttaatatt tttcgtgggc gaagtccagc cgaatcgcac gcaccatccg
     9721 caccggaggc cttgcatgaa accgacaacc atctcgttcc tcgtttcggg ccgtggatcc
     9781 aacttccagg cggtcgccgc ggcggtccgc gacggttccg tccccgcgac cctcgggatc
     9841 gtcatttcgg accgcgccgg ggccggggcg ctcgatatcg cccggcaatt cgggatgggg
     9901 gcgtttcatg tcgatgcgaa ggcgtatccc ggaaaacgtg aatacgaatc ggccatggtc
     9961 gagcttctgg agc
//
LOCUS       DAORHF010000130         9917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6142_length_9917_cov_5.542385, whole genome shotgun sequence.
ACCESSION   DAORHF010000130 DAORHF010000000
VERSION     DAORHF010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9917
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6142_length_9917_cov_5.542385"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /gene="aroE"
                     /locus_tag="PKJ16_11395"
     CDS             complement(<1..742)
                     /gene="aroE"
                     /locus_tag="PKJ16_11395"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HNV47636.1"
                     /translation="MTISASTALYGIFGNPVGHSLSPAMHNAAFLARGVDAVYLAFQP
                     QDAAAAVAAMRAIGMRGASVTIPFKTDMLRLVDSVDPLARDIGSVNTLANHGGVITGY
                     NTDGGGALRALRAAGITVAGSLALVLGNGGSARAIAHALLAEKALVTIAGRSLERVRP
                     LADDLRATHGGVSTALIGDITPEAAARYAIIINTTPVGMTPDTERIPIDAELIRSHHT
                     VFDIVYSPHETRLLRAARDRNARTVHGIE"
     gene            complement(739..1107)
                     /locus_tag="PKJ16_11400"
     CDS             complement(739..1107)
                     /locus_tag="PKJ16_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47637.1"
                     /translation="MEPTPISLQPDALWTVLARDEGHWRMGVYRPDAAAPEDIDTLER
                     HTCPELFIAVGGRVGLLLADETRERVIALHAGEALLVEDFHNGFRIDECAYFVVIERT
                     SFTTHYRDRATGAITRTVQV"
     gene            1253..2020
                     /locus_tag="PKJ16_11405"
     CDS             1253..2020
                     /locus_tag="PKJ16_11405"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HNV47638.1"
                     /translation="MPKKQAGGPKKKTASARKTTGAAKKTATKKTAGKPAAPPARSYT
                     PLYVAIIMVLLIVILFLVNRQIGERGIIGQLDRGKKTETKDSRPAASADNSGKEQTAR
                     SDDGSRTADPGAPVAEKRAIKLFLYAFNEKTEKLTLTTVGRSIETREPVRDAIRELAK
                     GPSAREERQGLLSAVPKNLRVNSVRVANGTAVIDVNGAIEENANGSILLNRIDQIVYT
                     ATQFDGVKAVEITVNGKKRRFLGGDGLSVGGALRRHR"
     gene            2028..2552
                     /gene="cyaB"
                     /locus_tag="PKJ16_11410"
     CDS             2028..2552
                     /gene="cyaB"
                     /locus_tag="PKJ16_11410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HNV47639.1"
                     /translation="MLEIEIKAYCDDLARVRATVIDRGGRFVRDSVERDIYFNHPARD
                     FGATDEALRIRAVDGRFVLTCKGPKIGERAKSRYEREVVIDDADGMEEILVRLGFSVT
                     DRVEKRRSVYQLGDVEVCFDDVAGAGTFVELERKGMERVAIEDELFRLAAELGLERFE
                     RRSYLDLVMERRAT"
     gene            complement(2553..4949)
                     /gene="pheT"
                     /locus_tag="PKJ16_11415"
     CDS             complement(2553..4949)
                     /gene="pheT"
                     /locus_tag="PKJ16_11415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HNV47640.1"
                     /translation="MWLSLNIIRDMVDLDGIAPEEIAARLTMATAEIDGIDAVNRHFE
                     TVVTAKIIDVMKHPNADKLTVCDLDAGKEKLTVVCGAPNHKKGDIVALATVGTKLSEE
                     FTVTETKVRGVNSPGMLCSEKELGLSDDHSGIMIFPADTPIGVPLSRLYPDRVDVRLE
                     IDNKSITHRPDLWCHAGFAREIAALFGRAFKHPVDLSIAKTFTNSEPLTVTIHAPEAA
                     PRYCGLVVKNIRIEESPEWLKSRVSAIGMRPINNVVDVTNYVMAEIGEPMHAFDRKKL
                     RGNEIIVRTATDGEPMKTLDEQEHTLTAHDVVIADAGGPIALAGVMGGGDSEIDDATT
                     EIVLEAANFNPVNIRRTANRYGLRTEAAIRFEKSLDPEICPAAIVRCYELIKRLVPEA
                     EAVTEIVDAYPRKHSENRIETSSAFIRKKLGQDIDDARITGILSSLGFGVRDSGGDLD
                     ITAPTYRSTKDVSIPDDIVEEVGRIFGYDNITPVPPMVPCAAPARNEFRLFERRLKTI
                     LSRDYGLTEVSNYSFVGEAPLAALDLDEGLELRLRNPLSQEQDRLRRSLVPNIVGNIA
                     FNQRYSDAFRFFELGRVYIKKSRTDAELASEQTRITGCVYAKTPASPLFYDARTALTG
                     LIGELRIRGVQLRPIDSGLPPYAHPGRSLEIVIEGKHAGLVFELHPAVRQRFDIAGQA
                     ALFDVSVDQLFAAKKLERSFTELRKYPDVPFELSLIADRFAYAEEICAIAAKSSREFV
                     RDASVVSVYSGEQIPAGKKSVSVRVVFAAQDRTLAPEEIEKLQKGVIDALGKKGYALR
                     "
     gene            complement(4963..5994)
                     /gene="pheS"
                     /locus_tag="PKJ16_11420"
     CDS             complement(4963..5994)
                     /gene="pheS"
                     /locus_tag="PKJ16_11420"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HNV47641.1"
                     /translation="MKDTLETLQTNTLAAIRSATTAAELEEIRIRVLGRKGELTGILR
                     GLGGLAPEERAAVGKISNEIKADIETALGAKKTDLDGAAIGAMLAEEWIDVSLAGPAS
                     SKRPERGHLHPISQIQYEIEDIFTSMGFEILDGPEVETEYNNFEALNIPDHHPARDMQ
                     DTFWTEDGNLLRTHTSAIQVRGMATHRPPFRVIGPGRVFRYESTDASHENTFYQIEGM
                     MVDRNISVANLIYFMKILLKEIFRREVTIRLRPGYFPFVEPGFELDIHCLICGGDGCS
                     VCKQSGWVELLPCGLVHPNVLRYGNIDPAEWSGFAFGLGLNRLVMMRYGINDIRHFLS
                     GDLRFLYQF"
     gene            6255..9014
                     /locus_tag="PKJ16_11425"
     CDS             6255..9014
                     /locus_tag="PKJ16_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47642.1"
                     /translation="MRLKSMEIFGFKSFADKTKVVFEPGVTVIVGPNGCGKSNIVDAL
                     KWVLGEKQARNIRGEKMEDVIFNGTEQRKPLSVAEVTMAIDNASRVLGYDSDTVVVTR
                     RVFRDGESEYLINKASVRLKDIEQLFMDTGIGKSSYSIMEQGKIDMILSTRAEDRRYI
                     FEEAAGISRYKMQRRDSHKKLEETNANLQRINDIISEIARERDAKSNQAEKTKQYLKL
                     KNEHTDLDVRLNVMKARELAARREKLGADIERCTMEREAISSRVSAISAENERDEKNK
                     NDIQLQLFELDKELHTFRIRVTDIDDKSSKNRRLIEEQKQRLAAIERTVEERLRVEEG
                     LSEEIGNNRRAAEALSKTLEEDRARLAALSESRTAMNEGIRKERGVIEQARADIEASE
                     RRVRDLRNRIEVVLKRLIDAIEQRKAELTGSESERQEVRRRIDERLASLEGAIERAIG
                     QVESGSAADAVVTLRAVDVRGLADDIGRFEGYEDGFRSILFDKTGIHAEKEELDRAVK
                     LEADRIVALRDRISASELVIKRHQGDLETLAQDMSALEKEISRNESEINWMMKNVQSL
                     EGQIADIRRQIAGYREDSVKTAKLMTDLQAEIEEWERNIVAFNEKSKTLVEDINVLVK
                     NRDTLDRSIVDRKQLSKRDSDRLRSIVERIGDLDKSLIEVDYKLNGIGEYLWTEYERK
                     IEEFASLQIDEAAHAETQRRLADLKREIQSLGPINNLAIEEYKGLQKRYEYYTDQKRD
                     IEKARDDIYSVIEDINRTSIEMFTKTYIDIRRNFSEIFKQLFEGGQATLELTDPENVL
                     ESGIEIMVRPPGKKPKSIDLLSGGERALVAVALLFATYMEKPSPFCFLDEIDAPLDEQ
                     NIGRFIKMLREFTLQTQFVIVSHNKKTMSTGESIYGVTMEEPGVSKVVSLRMEKVEVK
                     PAGVT"
     gene            9159..9725
                     /locus_tag="PKJ16_11430"
     CDS             9159..9725
                     /locus_tag="PKJ16_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47643.1"
                     /translation="MKVSIDGILGSARKLNTQKRTEDDSSEKKKAPVAADRVSIGSKV
                     ASRLDSIQRELREVQTSLTRNQIIDDGIRQLREDLGRGSQNSARIFDEVRFGPAKVLH
                     DFVGDSVTSDILDAKQERLRSLVDGDIGRLRRLQVESENILASDMAQPAAVDSILRNI
                     DSVFTEQGAQALERSSRLNADAVMRLIK"
     gene            complement(9734..>9917)
                     /locus_tag="PKJ16_11435"
     CDS             complement(9734..>9917)
                     /locus_tag="PKJ16_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47644.1"
                     /translation="IMQHDTGADLQSGMVGFCCQNTQMAIGEFSASDGTAIMLHDDFT
                     TESIKRHRVIATPVEK"
BASE COUNT         1931 a         2967 c         3327 g         1692 t
ORIGIN      
        1 tctcgatgcc atgaacggtc cgcgcgttcc ggtcacgcgc cgcgcgcagg aggcgggtct
       61 cgtgcgggct gtacacgatg tcgaacaccg tatggtgcga tcggatgagc tccgcgtcga
      121 tgggtatgcg ctcggtgtcg ggcgtcatcc cgaccggggt ggtgttgatg atgatcgcgt
      181 agcgcgcggc ggcctcgggc gtgatgtcgc cgatgagcgc ggtggagacg ccgccgtggg
      241 tcgcgcggag atcgtcggca aggggccgca cgcgctccag ggaccgcccc gcaatggtta
      301 ccagcgcctt ttccgcgaga agcgcatgcg caatcgcacg cgccgagccg ccgttaccca
      361 acaccagcgc gagggagccc gcgacggtaa tgccggccgc gcgaagggcg cggagcgccc
      421 cgccgccgtc ggtgttgtag ccggtgatga cgccgccgtg attggccagg gtgttcacgg
      481 agccgatgtc gcgcgccagc ggatcgaccg aatcgaccag gcgcagcatg tcggtcttaa
      541 acggaatggt cacgctcgcg ccgcgcatgc cgatggcgcg catcgccgcg accgccgccg
      601 ccgcgtcctg cggctggaag gccaggtaga ccgcatcgac gccccgcgct aaaaaggcgg
      661 cattgtgcat ggccggcgag agcgagtggc cgacgggatt gccgaaaatt ccatacagcg
      721 cggtcgaggc ggagatcgtc atacctggac cgtccgggtg atggcgccgg tcgcgcgatc
      781 gcgatagtgg gtcgtaaacg aggtgcgctc gatcacgacg aagtaggcgc actcgtcgat
      841 gcgaaagccg ttatggaagt cctcgaccag caaggcctcg cccgcgtgca gggcgatgac
      901 gcgctcgcgc gtttcgtcgg cgagcagaag accgacgcgc ccgccaacgg cgatgaagag
      961 ctccgggcag gtgtggcgct cgagcgtgtc gatgtcctcg ggcgcggcgg cgtcgggacg
     1021 atagacgccc atgcgccaat gcccctcgtc gcgagcgagg accgtccaga gcgcgtcggg
     1081 ctgcagggaa atgggcgtcg gttccatgcc cgcatgtggg ggcagcgcgg ggaattcatc
     1141 aagatttttt ttgcatgcgg gcgattttct gctatgccga tggtccgcat gaccgaatga
     1201 tacatcaagc cggagcgatt gcgcgccggg atcgaaacgg ggcgggtgga tcatgccaaa
     1261 gaagcaagcg ggcggaccga agaaaaaaac ggcctcggcg cggaagacga cgggcgcggc
     1321 gaagaagacc gcgacaaaaa agaccgcggg gaaaccggcg gcgccgccgg cgcgatcata
     1381 cacgcccctc tatgtcgcca tcatcatggt gctcctcatc gtgatcctgt ttctcgtcaa
     1441 ccggcagatc ggggagcggg gcatcatcgg gcaactcgat cgcggaaaga aaacggagac
     1501 gaaggattcg cgccccgcgg cgtccgccga taattcaggc aaggagcaga cggcgcgttc
     1561 ggacgatggg agtcgaacgg ccgatcccgg cgcgcccgtc gcggagaagc gcgccatcaa
     1621 actgtttttg tatgcgttca atgagaagac cgagaagctc acgctcacga cggtcgggcg
     1681 aagcattgaa acgcgcgaac cggtgcgcga cgccatccgc gagctggcca agggccccag
     1741 cgcgcgcgag gagcgccagg gactcctgag cgcggtcccg aaaaacctgc gggtcaattc
     1801 ggtgcgcgtg gccaacggga ccgccgttat cgacgtcaac ggcgcgatcg aggagaacgc
     1861 gaacgggagt atcctgctca atcgcatcga ccagatcgtg tacaccgcca cgcaattcga
     1921 cggcgtgaag gcggtcgaaa tcaccgtaaa cggaaagaag cggcgctttc tcggcggcga
     1981 cggcctctcg gtgggcggcg cgcttcggcg gcaccggtag gttctccatg ctcgagatag
     2041 agatcaaggc gtactgcgac gatctcgccc gggtgcgggc gacggtgatc gaccggggcg
     2101 ggcgcttcgt gcgcgattcg gtcgagcgcg acatctactt caaccacccc gcgcgcgatt
     2161 tcggcgcgac cgacgaggcg ctccggatcc gcgcggtgga cgggcggttc gtcctcacct
     2221 gcaagggccc gaagatcggc gagcgcgcga agagccgcta cgagcgcgag gtcgtcatcg
     2281 acgacgccga cggcatggag gagatactgg tgcgcctcgg cttttccgtg accgaccggg
     2341 tcgagaagcg gcgctcggtg tatcagctgg gcgatgtcga ggtgtgcttc gacgatgtcg
     2401 ccggtgcggg gacgttcgtg gagctcgaga ggaaaggaat ggagcgcgtc gcgatcgagg
     2461 atgagctgtt tcgcctggcc gccgagctgg ggctggagcg cttcgagcgg cgttcgtatc
     2521 tggacctcgt gatggagcgg cgggcgacgt gattaccgca atgcgtaccc cttcttcccc
     2581 agcgcgtcga tgacgccctt ctgcagcttc tcgatctcct ccggagctag cgtccggtcc
     2641 tgcgccgcga agaccacccg cacggaaacg gacttcttgc ccgcggggat ctgctcgccc
     2701 gaatagaccg ataccaccga tgcgtcccgg acgaactcgc ggctgctctt cgcggcgatc
     2761 gcgcaaatct cctcggcata ggcgaagcgg tccgcgatga gcgacagctc gaacgggacg
     2821 tccggatatt ttcgcagctc cgtaaacgag cgctcgagct ttttcgccgc gaagagctgg
     2881 tcgacgctca cgtcgaacag cgccgcctga ccggcgatgt cgaagcgctg gcgaacggcg
     2941 ggatggagct cgaagacgag ccccgcgtgt ttgccctcaa tcacgatctc gaggctccgc
     3001 ccgggatgcg catagggcgg gaggcccgag tcgattggcc tgagttgaac gcccctgatc
     3061 cgaagctcgc cgatgagccc ggtgagggcc gtgcgcgcgt cgtagaagag cggtgatgcg
     3121 ggcgtcttcg catacacgca gccggtgatg cgcgtttgtt cgctggccag ttccgcatcc
     3181 gtgcgcgact tcttgatata cacccgcccc agctcgaaga agcgaaacgc gtcgctgtag
     3241 cgctggttga atgcgatatt ccccacgatg ttcgggacga gcgagcggcg gagtcggtcc
     3301 tgctcctgcg agagaggatt gcgcaggcga agctccagac cctcgtccag gtccagcgcc
     3361 gcgagggggg cctcgccgac gaaggagtag ttgctcacct ccgtgagccc gtagtcgcgc
     3421 gagagtatcg tcttgaggcg ccgctcgaac agtcggaact cattgcgggc cggcgccgcg
     3481 cagggcacca tcgggggaac gggggtgata ttgtcgtagc cgaagatgcg ccccacctcc
     3541 tccacgatat cgtcgggtat ggagacgtcc ttggtcgatc ggtaggtggg cgccgtaatg
     3601 tcgagatcac cgccgctgtc gcgcacgcca aaaccgagcg acgagaggat gccggtgatc
     3661 cgcgcgtcgt cgatatcctg cccgagcttc tttctgatga acgcgctcga cgtctcgatt
     3721 ctgttttccg agtgtttacg gggataggcg tcgacgattt cggtgacggc ctcagcctcg
     3781 gggaccaggc gcttgatgag ctcatagcag cgaacgatcg ctgccgggca gatctccggg
     3841 tcgagggact tctcgaagcg gatggcggcc tcggtgcgca ggccataccg gttcgcggtg
     3901 cggcgtatgt tcacggggtt gaagttcgcg gcctcgagca cgatttcggt ggtggcatcg
     3961 tcgatctcgc tgtcgccgcc gcccatcacc ccggccagcg cgatcgggcc gcccgcgtcg
     4021 gcgatcacca cgtcgtgtgc cgtgagcgtg tgctcctgct cgtcgagcgt tttcatgggc
     4081 tcgccgtcgg tcgcggtgcg gacgatgatc tcgttcccgc gcagtttttt ccggtcgaaa
     4141 gcgtgcatcg gctcgccgat ctccgccatc acgtaattgg tcacgtccac gacattgttg
     4201 atgggccgca tgccgatcgc cgacacgcgg ctcttgagcc attccggcga ttcttcgatg
     4261 cggatgttct tgacgaccag tccgcagtat cgcggcgcgg cctcgggcgc gtgtatggtc
     4321 accgtcagcg gttcggagtt cgtaaacgtt ttcgcgatcg aaaggtcgac cgggtgcttg
     4381 aatgcgcggc cgaagagcgc ggcgatctcg cgcgcgaagc ccgcatggca ccacaggtcc
     4441 ggacggtgcg tgatggactt gttgtcgatc tccaggcgta cgtccacccg gtccggatag
     4501 agccgtgaga gcgggacgcc gatgggcgtg tccgccggaa aaatcatgat gccgctgtgg
     4561 tcgtcggaga ggccgagttc cttctctgag cagagcatcc cgggcgagtt gacgccgcgc
     4621 accttcgtct ccgtgaccgt gaattcctcg gagagcttcg tgcccacggt cgcgagcgcg
     4681 acgatgtcgc cttttttgtg attgggcgcg ccgcagacga cagtgagctt ctccttcccg
     4741 gcgtcgagat cgcagaccgt gagcttgtcg gcgttggggt gcttcatcac gtcgatgatc
     4801 ttcgccgtga cgaccgtctc gaaatggcgg ttcacggcat cgatcccgtc gatctctgca
     4861 gtggccatgg tgagtcgcgc ggcgatctcc tccggtgcga tgccgtcgag atcgaccatg
     4921 tccctgatga tgttaagcga gagccacatg ggtcacctgc cgttagaatt gatagaggaa
     4981 gcgcagatct ccgctcagga agtggcggat gtcgttgatg ccgtagcgca tcatcaccag
     5041 ccggttgagg cccagtccga aggcgaagcc cgaccactcg gcgggatcga tgtttccgta
     5101 gcggagcacg ttggggtgca ccagcccgca ggggaggagc tccacccagc cgctctgctt
     5161 gcacacgctg cagccgtccc cgccgcagat gaggcagtgg atgtccagct cgaagccggg
     5221 ctccacgaag gggaagtagc ccgggcgcag gcggatggtg acctcgcggc gaaagatttc
     5281 cttgaggagg atcttcatga agtagatgag gttggccacc gagatgttcc ggtccaccat
     5341 catgccctcg atctggtaga acgtgttttc atgggacgcg tcggtggatt catagcggaa
     5401 cacgcggccg ggtccgatca cgcgaaacgg cgggcggtgc gtcgccatgc cgcgcacctg
     5461 gatggccgag gtgtgcgtgc gcagcaaatt gccgtcctcg gtccagaagg tgtcctgcat
     5521 gtcgcgcgcg ggatggtggt ccgggatgtt gagcgcctcg aagttgttgt actcggtttc
     5581 cacctcgggc ccgtcgagga tctcgaagcc catcgaggtg aagatgtcct cgatctcgta
     5641 ctggatctgc gagatggggt gcaggtgccc gcgctcggga cgcttcgacg atgcgggtcc
     5701 ggcgaggctc acgtcgatcc attcctcggc gagcatcgcg ccgatggcgg cgccgtcgag
     5761 gtcggtcttt tttgcgccga gggccgtttc gatatccgcc ttgatttcgt tggatatctt
     5821 cccgacggcg gcgcgctcct ccggcgcgag gccccccagg ccgcgcagga tgccggtgag
     5881 ctcgcccttg cgtccgagca ccctgatgcg gatctcctcg agctccgccg cagttgtcgc
     5941 gctccgtatg gccgccaggg tgtttgtctg caatgtttcg agtgtgtctt tcatgccggg
     6001 tccgttcgtc tgctgcaata tcgctatcaa catcccttta aagaagaaac gtgagacaat
     6061 gctaggaggg cgaagactcc tgacaagaaa aaaatggcgc gcccgaggcc gcatgcccgg
     6121 aatatttgcc gggaagcgaa aaatgtgctt gccgaaaatc gcgcggggcg gcacctgaat
     6181 gcagcgcggg tttgtttcac agagacaccc acccctggcg cacaccaacc aactaacgga
     6241 actggcgaag cgagatgcgt ctcaaatcga tggaaatatt cggcttcaag tcattcgccg
     6301 acaagaccaa agtcgtcttc gagccgggcg tgaccgtcat cgtcggcccc aacgggtgcg
     6361 ggaagtcgaa catcgtcgac gcgctcaagt gggtgctggg agagaagcag gcgcgcaaca
     6421 tccgcggcga aaagatggaa gacgtcatct tcaacgggac cgagcagcgc aagcccctct
     6481 cggtggccga ggtgaccatg gccatcgaca atgccagccg ggttttgggg tatgattccg
     6541 atacggtcgt ggtgacgcgc cgcgtatttc gcgacgggga atccgaatac ctcatcaaca
     6601 aggcgtcggt gcggctcaaa gacatcgagc agctgttcat ggacacgggc atcggcaaga
     6661 gctcatactc catcatggag cagggcaaaa tcgacatgat cctctccacg cgcgccgagg
     6721 accgccgcta catcttcgag gaggcggccg gcatctcgcg ctacaagatg cagcggcgcg
     6781 attcgcacaa gaagctcgag gagaccaacg ccaacctcca gcgcatcaac gacatcatct
     6841 ccgaaatcgc ccgcgagcgc gacgcaaaat ccaaccaggc cgagaagacc aagcaatacc
     6901 tgaagctgaa aaacgagcac accgatctcg acgtccgcct gaacgtgatg aaggcgcggg
     6961 agctggccgc gcgccgcgaa aagctgggcg ccgacatcga gcggtgcacg atggagcgcg
     7021 aggccatatc ctcgcgcgtc tccgcgatct cggccgagaa cgagcgcgac gagaagaaca
     7081 agaacgacat ccagctccag ctcttcgagc tcgacaagga gctccatacc ttccgcatcc
     7141 gggtcacgga catcgacgac aagtcgagca agaaccggcg gctcatcgag gagcagaagc
     7201 agcgcttggc ggccatcgag cgcacggtgg aggagcgcct tcgcgtcgag gaggggctgt
     7261 ccgaggagat cgggaacaac cggcgcgcgg ccgaggcgct gtcgaaaacg ctcgaggagg
     7321 accgcgcgcg tctggcggcg ctctcggaat cgcgcacggc gatgaacgag gggatccgga
     7381 aggagcgcgg cgtcatcgag caggcgcggg ccgacatcga ggcgagcgag cggcgcgtgc
     7441 gcgatctgcg caaccgcatt gaggtggtcc tcaagcgcct catcgacgcg atcgagcagc
     7501 gcaaggccga gctcaccggc tccgagagcg agcgccagga ggtgcgccgg cgcatcgatg
     7561 agcgtctcgc gtcgctcgag ggcgccatcg agcgcgcgat cgggcaggtc gagtcgggaa
     7621 gcgccgcgga cgcggtcgtg acgctgcggg cggtcgatgt ccgcgggctc gccgacgaca
     7681 tcggccgctt cgagggttat gaggacggct tccgttcgat cctgttcgat aaaaccggca
     7741 tccacgccga gaaggaggag ctggaccgcg ccgtgaagct cgaagcggac cgcatcgtcg
     7801 cgctgcgcga ccgcatctcc gcgagcgagc tcgtcatcaa gcgccaccag ggcgatctcg
     7861 agacgctcgc ccaggacatg agcgcgctcg aaaaggagat ctcgcgaaac gaaagcgaga
     7921 tcaactggat gatgaaaaac gtccagtcgc tcgaggggca gatcgccgac atccgacgcc
     7981 agatcgcggg gtatcgcgag gattccgtca agaccgcgaa gctgatgacg gacctgcagg
     8041 ccgagatcga ggaatgggag cggaatatcg tcgccttcaa tgaaaagagc aagacccttg
     8101 tcgaggacat caacgtgctg gtgaaaaacc gcgacaccct cgaccgcagc atcgtggacc
     8161 gcaagcagct gtcgaagcgc gactccgacc ggttgaggag catcgtcgag cggatcggcg
     8221 atctcgacaa gtcgctcatc gaggtcgatt acaagctgaa cgggatcggc gaatacctct
     8281 ggacggaata cgagcggaag atcgaagagt tcgcctccct gcaaattgac gaggccgcgc
     8341 atgccgagac ccagcgccgc cttgccgacc tcaagcgcga gatacagtcg ctgggtccca
     8401 tcaacaacct ggccatcgag gagtacaagg ggctccagaa gcgctacgag tattacaccg
     8461 accagaagcg cgacatcgaa aaggcgcggg acgacatcta ttcggtcatc gaagacatca
     8521 accgaacctc gatcgagatg ttcaccaaga cctacataga catacggaga aatttctcgg
     8581 agatattcaa gcagctcttt gagggcggcc aggcgacgct ggagctcacc gacccggaga
     8641 acgttctcga gagcgggatc gagatcatgg tgcgcccgcc cggcaagaag cccaagagca
     8701 ttgacctgct ctccggtggc gagcgggcgc tggtcgcggt ggcgctgctg ttcgccacct
     8761 acatggagaa gccgtctccc ttctgcttcc tcgacgagat cgacgcgccg ctggacgagc
     8821 agaacatcgg gcgcttcatt aaaatgctgc gcgagttcac gctgcagacg cagttcgtga
     8881 tcgtatcgca caacaagaag acgatgagca ccggcgagtc gatttacggg gtcacgatgg
     8941 aggagcccgg cgtgtcgaag gtggtgtcgc tgcgcatgga gaaggtcgag gtgaaacccg
     9001 ccggggtgac gtgagcgacg gaaataatta tgtcacatta cattaattat ttgacaaaaa
     9061 atcgatattt gatatattct ggctaatgcc ccgcgggatg gcgcgttagg acgcgcgtgc
     9121 ggggtgatgg aataccgctt cacaagggaa ggtgtacaat gaaggtatcc atagacggca
     9181 ttctgggttc cgcacggaag ttgaatacgc agaagcgaac cgaagacgac agttcggaaa
     9241 aaaagaaggc gcccgtcgcc gcggaccgcg tttccatcgg cagcaaggtc gcctcgcggc
     9301 tcgattccat ccagcgggag cttcgcgagg tgcagacatc gcttacccgc aatcagatca
     9361 tcgatgacgg catccgccag ctgcgcgagg acctcgggcg cggatcgcag aactcggcgc
     9421 gcatcttcga cgaggtccgg ttcggcccgg ccaaggtgct gcacgacttt gtcggcgatt
     9481 ccgtcacaag cgatatcctc gacgcgaagc aggagcggct gcgctcgctc gtcgatgggg
     9541 acatcggcag gctcaggcgg ctgcaggtcg agagcgaaaa catcctggcg tccgacatgg
     9601 cgcagccggc ggcggtcgat tcgatcctcc gcaatatcga cagcgtcttt accgagcaag
     9661 gcgcgcaggc gctggagcgc tcatcgcggc tcaatgcgga cgccgtcatg cggctcatca
     9721 agtagggcgg gggttacttt tcgaccggag tggcgatcac gcggtggcgc ttgatggatt
     9781 cggtcgtgaa gtcgtcgtgg agcatgatcg ccgttccgtc agaagccgag aattccccga
     9841 tcgccatctg cgtgttctgg cagcaaaatc ccaccatgcc gctttgaagg tcggcgccgg
     9901 tatcgtgttg catgata
//
LOCUS       DAORHF010000131         9814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6252_length_9814_cov_4.291013, whole genome shotgun sequence.
ACCESSION   DAORHF010000131 DAORHF010000000
VERSION     DAORHF010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6252_length_9814_cov_4.291013"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..1040
                     /gene="hypE"
                     /locus_tag="PKJ16_11440"
     CDS             51..1040
                     /gene="hypE"
                     /locus_tag="PKJ16_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005985138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HNV47645.1"
                     /translation="MKILPGHGSGGRLMNELIEKTIRTRLGAHVQLDDAALLSIDGPI
                     AFTTDSFTITPIFFPGGDIGALAVNGTVNDLAVMGADPRYLSCALIIEEGMEMADLER
                     ILDSMRAAADAAGVAIVTGDTKVVPRGAADRIFINTSGIGIVKKTPARREISDGDAIL
                     INGHIGDHGMAIMAARNNLSFSKGLQSDCASLKGMIGAVLERHPGSVKFMRDATRGGV
                     ASVLNEIVAGRPYGAKLREPSLPIRDAVKGVCGILGIDALYAANEGKAVMVVDNDAAE
                     SIVSVLREHPEGKDACIIGVIDGTHPGKAYLETAIGGRRILPLLTEEQLPRIC"
     gene            1066..1290
                     /locus_tag="PKJ16_11445"
     CDS             1066..1290
                     /locus_tag="PKJ16_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014788332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HNV47646.1"
                     /translation="MCLAVPMTVIRIDGQQAVVEQGGVETSVSAALAPDIMIGDKVIV
                     HAGFIIEKLNEEEARLIDEAWDEYAKANAT"
     gene            complement(1362..1730)
                     /locus_tag="PKJ16_11450"
     CDS             complement(1362..1730)
                     /locus_tag="PKJ16_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47647.1"
                     /translation="MAKKILIIDDDPDILDSIKAILDGNGFATVTAISGSAGVAAFKK
                     DAPDLVLCDMMMESIDEGMKVAAEIRTINSKVPMYLLSSIGAATASNIEVSKLGFNGV
                     FQKPVDPGMLVSSVKKALGM"
     gene            complement(1753..2232)
                     /locus_tag="PKJ16_11455"
     CDS             complement(1753..2232)
                     /locus_tag="PKJ16_11455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNV47648.1"
                     /translation="MNSAKVIIYGYGNPGRQDDGLGPALVERLEGEALPGVETRSAFQ
                     LNIEDAQTIADAGTVIFVDASMTGDGPFAFHEIVPSAQITFTTHAMSPGSVLALCQDL
                     FNPERRAYMLSIRGYEWEFREGFSAGASGNLEQAYAFVMEKLAECVSRVDGAVLSVH"
     gene            complement(2236..3687)
                     /locus_tag="PKJ16_11460"
     CDS             complement(2236..3687)
                     /locus_tag="PKJ16_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HNV47649.1"
                     /translation="MSRKITIEPVTRVEGHGKVTIHMDDAGKVSQTRFHIVEFRGFER
                     FAQGRPYWEMPVLVQRLCGICPVSHHLAAAKAIDVIAGAGTGEGLTPTGEKMRRLMHY
                     GQMFQSHALHFFHLVSPDLLFGADGDPAIRNVIGVALMHKDLAVQGVMMRKFGQEIIL
                     ATAGKKIHGTGAIPGGINKNLSVAERDSFLKGADPLNIEKMVSWAQGALDFFKDYHSK
                     NKELIDDFAVFPSNHLSLVRKDGALDLYHGVLRAVDTDGKKILNDVDYQDYLTYIGEE
                     VRSWSYMKFPYLKSLGKEKGWYTVGPLARLNTADFIPTPLAQKEFEIYKAYTKGKVNN
                     MSMHMHWARLIELLHSAEVIRDLLNDKDLQGDNLVAKGPKKKEGVGLVEAPRGTLFHH
                     YRVNDNDQVEMANLIVSTTNNNEPMNQAVNYVANWAFNGKKEITEGMMNRVEMAIRAY
                     DPCLSCATHALGQMPLEIELLGAGGIMLDRKRR"
     gene            complement(3709..4257)
                     /locus_tag="PKJ16_11465"
     CDS             complement(3709..4257)
                     /locus_tag="PKJ16_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HNV47650.1"
                     /translation="MSKPVVATASLAGCFGCHMSVLDIDDRILQLIDLVEFNKSPVDD
                     IKTFTKTCDIGIIEGGCCNSENVENLKEFRKHCKILISLGECAIMGGLPAMRNGIPVK
                     ECMEEAYYNCPTIDKNDPKIMPNDDELPMILDRVYPLHEIVKIDYYLPGCPPRADLIW
                     EALVALITGKELSLPYEVLKFD"
     gene            complement(4286..4978)
                     /locus_tag="PKJ16_11470"
     CDS             complement(4286..4978)
                     /locus_tag="PKJ16_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005505387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HNV47651.1"
                     /translation="MADITLTIDGKTCKAKPGQSIVDAAKENGIYIPVLCNYEGLKPA
                     GTCRICTVRVGGRFMAACTTPVGEGMIVESGTPDIEDMRKAIVEMLFVEGNHMCPCCE
                     KSGNCELQALGYRYQMLVPRFPYLWPASQVDASTPKIFLDKNRCVQCLRCVRGAVTPD
                     GKHVFGLLNRGDRTAIAIDHALASKMTDEMAQKAMDSCPVGAILRKEVGYVVPIGMRK
                     FDSKPIGSDIGQ"
     gene            complement(4980..6794)
                     /locus_tag="PKJ16_11475"
     CDS             complement(4980..6794)
                     /locus_tag="PKJ16_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV47652.1"
                     /translation="MGSHIASVVQKAADRYRNDSSKMLDVIRDVQSETGSVSEEAIVQ
                     IAAAMNVSQVDVEGVVTFYHFFSKSPVGKYSVYLNNSAVSFMKGCAAIAKAFEQEAGC
                     AFGKTTADGNIGLFYTSCIGMNDQEPAAIINDVVFTNLTVEKVKEIVAGMKAGKSAKD
                     LVKQFGDGQNQSELVKSMVNNNIHKKGLVLFAPFEAGSAVKKVATMTPDAVVEEMKVS
                     NVRGRGGAGFPTGMKWDFTRKSEGPKRYIVCNADEGEPGTFKDRVILTEMPQMLFEGM
                     AVGGYAIGADEGLLYLRAEYMYLKNYLENVLEKMRKNNQLGKDIAGKKGFNFDIKIQM
                     GAGAYVCGEESALIESAEGKRGQPRNRPPFPAQVGFMGKPTSVNNVETFCCAARIVLE
                     GGAWFAAMGTLQSKGTKLLSVSGDCKKPGIYEIEFGTTVKTLLEECGAMDAVAVQIGG
                     PSGTCIGKKDFGRRICYDDLATGGSIIVMGPKTDILQVVHNFMEFFVEESCGWCVPCR
                     AGNKILTERLEKIMHGKGSATDLTELESWCAIVKSMSRCGLGQTSPNPILTTIQNFRE
                     LYEAKIQKGKDYVSEFDFAAAVKESCAVANRKPLSEGH"
     gene            complement(7118..7585)
                     /locus_tag="PKJ16_11480"
     CDS             complement(7118..7585)
                     /locus_tag="PKJ16_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV47653.1"
                     /translation="MADMNLGSLLDKAIKNEEDAHAFYMNLHGIVSDADAKSTLLFLA
                     DQEKKHKEFLVAYKSGSYANKALAANTAIDYAIAQYVDAPDIEKNMKTKDVYLVAAHR
                     ELNSYNFYKGLAAVQPAGEVKDVLVRMADEELKHKEKVEYLYANSAFPQTEGG"
     gene            complement(7673..8962)
                     /locus_tag="PKJ16_11485"
     CDS             complement(7673..8962)
                     /locus_tag="PKJ16_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HNV47654.1"
                     /translation="MSNENLESKRWLIAGAAIVMQLCLGTVYAWSVFKKPLMGTHGWA
                     ETPTQLTFMILMLVIGCSAAFGGTLVDKKGPRFVATIGGILFGVGTLIAGLADQMGNL
                     YLLYLGFGIVAALGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGQIAPF
                     MINKFQVLGEGGKIVSSGVATTFYIWGAIFLVLVTGAAQFYKNPPQGWVPAGFTPAKS
                     AVSAADSYQFGQAVKVPQWWMLWAMLFLNVSAGLGLISQLSPLAQEMYKAYVPADLTG
                     DAVVKALAVAGGTVVAITAIFNGLGRLFWASLSDKIGRRGVFMTMFISQAVLYVLVAA
                     GIISNYYLFLIAGCYLLACYGGGFATMPAFAADAFGPTYIGKVYGFMLTAWSCAGLVG
                     PFVFAQFKAQALYIAAGLLVVGFLIALIYKKPERKAA"
     gene            complement(9053..>9814)
                     /locus_tag="PKJ16_11490"
     CDS             complement(9053..>9814)
                     /locus_tag="PKJ16_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HNV47655.1"
                     /translation="GLRGYPRFKPPFPGGAGFNNLPSNVNNVETFASVPLIMKNGADW
                     YKAVGPNNCAGTKLFCLSGKLNRTGLVELPMGATLKEIIGVYGKGIKGGKKFKFAQVG
                     GTAGGILGKDLMDLPLDIDQTVKAGVTLGSGVVLVCDESTCAVDYLLHILSFFEHESC
                     GQCVPCRVGTSHLHHLVKKIASRTASVDDIALLEEKALLMKKASLCALGQSPIMPITT
                     ILKYFRKEFEKHCDPKYACKACDASLRRYYAGAAH"
BASE COUNT         1915 a         3008 c         2912 g         1979 t
ORIGIN      
        1 tccaccgaag ggccctgcgc ggcattctac aagtatggag gattcgaccg atgaagatcc
       61 ttcccggcca cggcagcggc ggcaggctca tgaacgagct catcgaaaag acgatccgca
      121 cgcgacttgg cgcacacgtt cagctcgacg acgccgccct cctgtcgatc gacgggccga
      181 tcgccttcac caccgacagc ttcaccatca ccccgatctt ctttccgggc ggcgacatcg
      241 gcgcgctcgc ggtgaacggc accgtaaacg acctcgcggt gatgggcgcc gatccgcggt
      301 atctctcctg cgcgctcatc atcgaggagg ggatggagat ggccgacctc gagcggattt
      361 tagactcaat gcgcgccgcg gcggacgcgg cgggcgtcgc catcgtcacg ggcgacacca
      421 aggtggtgcc ccgcggggcg gccgaccgga tcttcatcaa caccagcggc atcggaatcg
      481 tgaaaaaaac gcccgcccgt cgtgaaatct cggacggaga cgctatactg ataaacggcc
      541 acatcggcga tcacggcatg gccatcatgg cggcgcgcaa caacctgagt ttttcaaagg
      601 gactgcaatc ggactgcgcg tcgctcaagg gcatgatcgg cgcggtgttg gagcgtcacc
      661 ccggcagcgt caagttcatg cgcgacgcca cgcggggcgg cgtcgcctcg gtgctcaacg
      721 agatcgtcgc cgggcggccc tatggcgcga agctgcgaga accgtcgcta ccgatccgcg
      781 acgcggtgaa gggcgtctgc ggcattctgg ggatcgacgc gctttacgcg gcaaacgaag
      841 ggaaggcggt gatggtcgtc gacaatgacg cggccgagtc gatcgtgagc gtgctgcgcg
      901 agcaccccga agggaaggac gcctgcatca tcggcgtcat cgacggaacg catcccggaa
      961 aggcctacct tgaaacggcc atcggcgggc ggaggatcct gcccctcctc acggaggagc
     1021 agttgccgag aatctgctaa cgacgattac tggaggaaga gcaccatgtg tctcgcggta
     1081 ccgatgacgg tgatcagaat cgacggccag caggccgtcg tggaacaggg cggggttgaa
     1141 acgagcgtga gcgcggcgct cgctcccgac atcatgatcg gcgacaaggt gatcgtccac
     1201 gccggcttca tcatcgagaa gctcaacgaa gaggaggccc gcctcatcga cgaggcttgg
     1261 gacgagtacg cgaaggcgaa cgccacatag acagcaaggg ccccccgccg cgcgtgtggg
     1321 gagcccttat cgtttctcgt gagccgttcg aacgacgccg gctacattcc cagcgccttt
     1381 ttaacgctcg agaccagcat gccgggatcg accggtttct gaaatacgcc gttgaatccg
     1441 agcttgctca cttcaatatt tgaagctgtc gcggcgccga tgctcgacag gagatacatc
     1501 gggactttgc tgtttatcgt gcggatctcg gccgcgacct tcatcccctc gtcgatgctc
     1561 tccatcatca tgtcgcagag caccagatcg ggagcatcct tcttgaacgc cgccacgccc
     1621 gcgctgccgc tgatggcggt cacagtcgcg aacccgttcc cgtcgagaat ggccttaatg
     1681 gaatcgagga tatcgggatc gtcatcgatg ataagaatct tttttgccat ataatgtctc
     1741 cttgctgttt tttcagtgta cggacaaaac cgcaccatcg acacgcgata cgcactcggc
     1801 cagtttttcc atcacaaacg cataggcctg ttcgagattg ccactggcgc ctgccgaaaa
     1861 cccctcacga aactcccact cataaccgcg aattgaaagc atatacgccc ttcgctccgg
     1921 gttaaagagg tcctgacaca gcgccaggac cgaaccgggt gacattgcgt gcgtcgtgaa
     1981 ggttatctgg gccgacggaa cgatctcatg gaatgcgaac ggtccgtcgc cggtcatcga
     2041 cgcatccacg aagatgaccg tcccggcatc ggcgatcgtt tgcgcgtctt cgatattgag
     2101 ctggaaggcg cttcgggtct cgacccccgg cagcgcctcg ccctcgagcc gctcgaccag
     2161 ggccggtccc aggccgtcgt cctgacggcc gggattgccg tacccgtaaa tgatcacttt
     2221 tgcgctattc aaaacctacc gtcgcttccg gtcaagcata ataccgccgg cgcccagcaa
     2281 ctcgatctcg agcggcatct gccccagcgc atgcgtggcg caggagaggc acggatcata
     2341 tgcgcggatc gccatctcga cgcggttcat catgccctcg gtaatctcct tcttgccgtt
     2401 gaacgcccag ttggcgacgt agttgaccgc ctggttcatc ggctcgttgt tgttcgtggt
     2461 ggagacgatg aggttggcca tctcgacctg gtcgttgtcg ttgacgcgat agtggtggaa
     2521 cagcgttccg cgcggcgcct cgaccagacc cacgccctcc ttcttcttgg gccccttggc
     2581 gaccagattg tcgccctgga gatccttgtc gttcagcagg tcgcgaatga cctcggccga
     2641 gtgcaagagt tcgatcaggc gcgcccagtg catatgcatg gacatgttat tgaccttgcc
     2701 cttcgtatag gccttgtaga tctcgaattc cttttgcgcc agcggcgtgg gaataaagtc
     2761 cgcggtattg agtcgggcca gcggaccgac ggtgtaccag cccttttcct tgccgagcga
     2821 tttgaggtac gggaacttca tgtagctcca cgaacggacc tcttcgccga tgtacgtcag
     2881 atagtcctga taatcgacat cgttcaggat cttcttgccg tcggtatcga ccgcgcgcag
     2941 tacgccgtga tacaggtcga gcgcgccgtc cttgcggacc agcgacaggt ggttcgacgg
     3001 gaacacggcg aaatcgtcga tgagttcctt gttttttgaa tggtagtcct tgaagaagtc
     3061 aagcgcgccc tgcgcccagg aaaccatctt ctcgatgttg agcggatcgg cccccttgag
     3121 aaagctgtcg cgctcggcga cggagaggtt cttgttgatg ccgccgggga tcgcgccggt
     3181 gccgtggatc ttcttcccgg ctgtggcgag aatgatctcc tggccgaact tgcgcatcat
     3241 gacgccctgc accgcgaggt ccttgtgcat gagcgccacg ccgatgacgt tccggatcgc
     3301 cggatcgccg tccgcgccga agagcaggtc gggcgatacg aggtggaaga aatgcagcgc
     3361 atgcgactgg aacatctggc catagtgcat gaggcggcgc attttctcgc cggtcggcgt
     3421 gaggccttcg ccggtaccgg cgcccgcgat cacgtcgatc gccttggcgg ccgctaggtg
     3481 atggctcacc gggcagatgc cgcagaggcg ctgcaccagg acgggcattt cccagtaggg
     3541 acgcccttgg gcgaatcgct cgaaaccgcg gaattcgacg atgtgaaaac gggtctgcga
     3601 caccttaccg gcgtcgtcca tatggatggt aaccttgcca tggccctcga cgcgggttac
     3661 cggttctatc gttatttttc tactcatgat gtgctccctg tcagtggatt agtcgaactt
     3721 gagcacttcg tacggaaggc tcagctcttt tccggtgatg agcgcgacca gcgcctccca
     3781 gatgaggtcc gcgcggggcg gacagcccgg cagatagtag tcgatcttca cgatctcgtg
     3841 cagggggtac accctgtcca agatcatcgg aagctcgtcg tcgttgggca tgatcttggg
     3901 atcgttcttg tcgatggtcg ggcagttgta atacgcctcc tccatgcact ccttgaccgg
     3961 aatgccgttg cgcatcgcgg gcaggccgcc catgatggcg cactcgccca gcgagatgag
     4021 gatcttgcag tgcttccgga actccttcag gttctccacg ttttcgctgt tacagcaccc
     4081 gccctcgatg atgccgatgt cgcaggtctt ggtgaacgtc ttaatgtcgt cgacgggcga
     4141 cttgttgaac tccacgaggt cgatcagttg cagaatgcga tcgtctatat ccaacacgga
     4201 catatggcac ccgaaacaac ccgccaggga cgccgttgcc actactggtt tactcattgc
     4261 atctcctccg tcactatcct gattgttatt gtccgatgtc gctgccgatc ggcttcgaat
     4321 cgaatttacg catcccgatc ggcaccacgt agccgacctc tttcctgagg atcgcgccca
     4381 ccggacacga atccatcgcc ttctgcgcca tctcgtcggt catcttcgat gcgagcgcat
     4441 ggtcgatggc gatggcggtg cgatcgccgc ggttcagcaa tccaaagaca tgcttgccgt
     4501 cgggcgtgac ggcgccgcgg acgcagcgca ggcactggac gcagcggttc ttatccagga
     4561 agattttcgg ggtgctggcg tcgacctgag acgcgggcca caggtacggg aaacggggca
     4621 ccagcatctg atagcgatac ccgagcgcct gcaactcgca gttgccgctc ttctcgcagc
     4681 aggggcacat gtgattcccc tcgacgaaga gcatctcgac gatcgccttg cgcatgtcct
     4741 cgatgtcggg cgtgccgctc tcgacaatca tgccctcgcc gaccggcgtg gtgcacgcgg
     4801 ccatgaagcg gccgccaacc cgcaccgtgc agatgcggca ggtgccggcg ggcttcagcc
     4861 cttcatagtt gcacagcacg gggatgtaga tgccgttttc cttggcggca tcgacgatcg
     4921 actgccccgg tttcgccttg caagtctttc cgtctatggt taatgtgata tctgccattt
     4981 tagtgcccct cgctcaaagg ttttctgttg gccacggcgc acgattcctt cacggccgcg
     5041 gcgaaatcga actcgcttac gtagtccttg cccttctgga tcttggcttc gtagagctcc
     5101 cggaagttct gaatggtggt gaggatggga ttgggagatg tctgaccgag accgcaccgg
     5161 ctcatcgact tgacgatcgc gcaccaggac tcgagctccg tgagatcggt cgccgatccc
     5221 ttgccgtgca tgatcttctc caggcgctcg gtgaggatct tgttgcccgc ccggcacggc
     5281 acgcaccagc cgcagctttc ctcgacgaag aactccatga agttgtggac cacctgcagg
     5341 atgtcggtct tgggccccat gacgatgatg gagccgccgg tggcgagatc gtcatagcag
     5401 atccgccggc cgaagtcctt cttgccgatg caggtccccg agggaccgcc gatctgcacc
     5461 gcgacggcgt ccatcgcccc gcactcctcc aggagcgttt tcacggtggt gccgaattcg
     5521 atctcgtaga tccccggctt cttgcagtcg ccggagacgg acagcagctt ggtgcccttt
     5581 gactgcagcg tccccatggc ggcgaaccac gcgccgccct cgagcacgat gcgcgccgcg
     5641 cagcagaagg tctcaacgtt gttcacggag gtgggcttac ccatgaaacc gacctgcgcc
     5701 gggaacggcg ggcggttgcg gggctggccg cgcttgccct cggccgattc gatgagcgcc
     5761 gattcctcgc cgcacacata ggcgccggcg cccatctgga ttttgatgtc gaagttgaag
     5821 cccttcttgc cggcgatgtc cttgccgagc tggttgtttt ttctcatctt ctcgaggacg
     5881 ttctcgagat agttcttcag gtacatatat tccgcacgga gatagaggag accctcatcg
     5941 gcgccgatcg cgtagccgcc gaccgccatg ccctcgaaga gcatctgcgg catctcggtg
     6001 aggatcacgc ggtctttgaa ggtgccgggc tcgccctcgt cggcgttgca gacgatgtag
     6061 cgcttgggcc cctcgctctt gcgggtgaag tcccacttca tcccggtggg gaaacccgcg
     6121 ccgccgcggc cgcgaacatt ggagaccttc atctcttcga ccacggcatc cggggtcatg
     6181 gtcgcgacct tcttgactgc ggaacccgcc tcgaacggcg cgaagagcac aaggcccttc
     6241 ttgtgaatgt tgttgttcac catggacttg acgagctcgg actggttctg gccgtcgccg
     6301 aactgcttca cgaggtcctt ggcgctcttg ccggccttca tgccggcgac gatctctttc
     6361 accttctcga ccgtgaggtt ggtgaaaacc acgtcgttga tgatggcggc cggctcctgg
     6421 tcgttcatcc cgatgcagga ggtgtagaag agaccgatgt tgccatcggc ggtcgttttg
     6481 ccaaacgcgc atcctgcttc ctgttcgaat gccttggcga tcgcggcgca gcccttcatg
     6541 aacgaaaccg cgctgttgtt cagatacacc gagtacttcc cgacgggcga ctttgaaaag
     6601 aagtgataga acgtcaccac gccctcgaca tccacctgcg acacgttcat cgccgcggcg
     6661 atttggacga tggcctcctc cgaaacgcta ccggtttccg attgaacgtc acggataaca
     6721 tccagcattt tcgaagagtc gtttcgatac ctgtcagccg ctttttgcac taccgaagca
     6781 atgtgtgaac ccatagaccc tcttttttga tggaattctc gctctgggca tagttcaccg
     6841 ttgatcgcat acgcaccgcg acgcgcagac acacctcgag cgccaatgac acgccagaac
     6901 gaatgttagg aaagaaaaaa cgaatggcta aaggggagat gtaaaagaac ctacacccgg
     6961 gaccgggtgc atttctattt agcaaccact gttacgatgc ccgtccatca tgcaactttt
     7021 ttttgttcag aaaaaatatt ttttctcctc ccggccaatc acccggcccg aaaacccaac
     7081 gagacaggcc gggtgacgtg cgcggcgacc ccggggatca ccccccctcg gtctggggga
     7141 acgccgagtt cgcgtagagg tattcgacct tttccttgtg cttgagctcc tcgtcggcca
     7201 tgcggaccag cacgtccttc acttctccgg ccggctggac cgccgccagg cctttgtaga
     7261 agttatagga attgagctcg cgatgcgcag cgacgaggta gacgtccttc gttttcatgt
     7321 tcttttcgat atcgggcgca tcgacgtact gcgcgatagc gtagtcgata gccgtgttgg
     7381 ccgcaagcgc cttgttcgca tacgacccgc tcttgtatgc aacgaggaac tccttgtgct
     7441 ttttctcctg gtcggccagg aacaggagcg tgctcttggc gtcggcgtcg cttacgatgc
     7501 cgtggaggtt catgtaaaac gcatgcgcat cctcctcgtt tttaatcgcc ttgtcgagca
     7561 gtgaaccgag attcatatcc gccatcgggt cctccatttt tgtaaaaatt gtcgataaaa
     7621 cgaaacaagg aaggcgccat gccttccttg tttcgcagaa aatgacaaac cgttacgcgg
     7681 ctttccgctc gggcttcttg tagatgagcg cgatcaggaa accgacgacc agcagaccgg
     7741 cggcgatata gagcgcctgg gccttgaact gggcgaacac gaagggaccc accaagccgg
     7801 cgcagctcca tgccgtgagc atgaacccgt acaccttgcc gatgtaggtg ggaccgaacg
     7861 cgtcggccgc gaaggcgggc atcgttgcga atccgccgcc atagcaggcg agcaggtagc
     7921 aacccgcgat caggaacaaa tagtaattgc tgatgatgcc ggcggccaca aggacataca
     7981 gcaccgcctg gctgatgaac atggtcatga acacgccgcg acggcctatc ttgtcggaaa
     8041 ggctggccca gaagagacgg cccagaccgt tgaagatggc cgtgatggcg acgaccgtgc
     8101 cgcccgcaac cgcgagggcc ttaacgaccg catcaccagt gaggtcggcc ggaacatagg
     8161 ccttgtacat ctcctgcgcg agcggagaaa gctgcgagat gaggccgaga cccgcggaca
     8221 cgttcaggaa gagcatggcc cacagcatcc accactgcgg aaccttgacg gcctggccga
     8281 actgatacga atcggcggcg gaaacagcgc tcttggcggg cgtgaagccg gccggcaccc
     8341 aaccctgggg cgggttcttg tagaattgcg cagcgccggt cacgagcacc aggaagatgg
     8401 cgccccagat atagaaggtg gtcgcgacgc cggagctcac gatcttcccg ccttcgccga
     8461 ggacctggaa cttgttgatc ataaacggcg caatctgccc catgaaaaag gcgcccgcgc
     8521 caaaacccat gaccgcaagg ccggtcacca gtccgcgctt atcggggaac caacggatca
     8581 gggtggcgat cggggtcaca tagccaaagc cgttacccag cgcggcgacg atgccgaagc
     8641 cgagatacag aagatagaga ttgcccatct ggtcggcaag gccggcgatg agcgtcccga
     8701 cgccgaacag gatgccgccg atcgtcgcga cgaagcgcgg gcccttcttg tcgacgagcg
     8761 tcccgccgaa ggcggcagaa catccgataa caagcatcag gatcataaat gtcagctggg
     8821 tcggggtctc ggcccagcca tgggtgccca tgaggggctt tttaaagacg gaccatgcat
     8881 acacggtccc gaggcaaagc tgcatgacga tcgccgctcc ggcgatgagc cagcgtttgc
     8941 tctcaagatt ttcattactc atgtgtttga ctcctcgtgt gaaattttat aaagcccgcg
     9001 ccccctccgc cggtccaccc ggcgaacggg gcggcaaata ccgacggtgc gcctagtgag
     9061 ccgcgccggc gtaataacgc cgcagcgagg cgtcgcacgc cttgcaagcg tacttcggat
     9121 cgcagtgctt ttcaaactcc ttgcgaaaat acttgagtat cgtcgtgatg ggcatgatgg
     9181 gcgactggcc cagcgcgcag agcgacgcct ttttcatgag caacgccttc tcctcgagca
     9241 gagcgatgtc gtcgaccgag gccgttcggg acgcgatctt cttgacaaga tggtgaaggt
     9301 gcgaagtgcc cacgcggcag ggaacgcact gcccgcacga ctcgtgctca aagaaagaga
     9361 gaatgtgcag caggtaatcg accgcgcagg tgctctcatc gcacaccaga accacgccgg
     9421 aacccagcgt cacgccggcc ttcaccgtct ggtcgatgtc gagcggcagg tccatgaggt
     9481 ccttgcccag gatcccgccg gccgttccgc cgacctgcgc gaacttgaac ttcttcccgc
     9541 ccttgatccc cttgccgtat acgccgatga tctccttgag cgtggcgccc atggggagct
     9601 ccaccaggcc cgtccggttg agcttgcccg agaggcagaa gagcttggtc ccggcgcagt
     9661 tgttcggacc tacggccttg taccaatcgg cgccgttctt catgatgagc gggaccgacg
     9721 cgaaggtttc cacattgttc acgttcgacg gaagattgtt gaagccggcg ccgcccggga
     9781 acggcggctt gaagcgcgga tagccgcgca ggcc
//
LOCUS       DAORHF010000132         9810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6256_length_9810_cov_4.738288, whole genome shotgun sequence.
ACCESSION   DAORHF010000132 DAORHF010000000
VERSION     DAORHF010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_9810_cov_4.738288"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PKJ16_11495"
     CDS             <1..478
                     /locus_tag="PKJ16_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47656.1"
                     /translation="GVAVVTPAPLGQTCDENGCHPCTHDERYSNLVTNLTANLPKLLR
                     KFQLTPENDGDAYMQYGETMYDELTQLGLIAFSPNGVGTYLLDNLKPASQYTAWNMTE
                     EIDSLLKKEIFTTSNEGTFWFELGHLLQSFGVIVKQREINNGQLLDYYSSFKGMFE"
     gene            complement(590..1474)
                     /locus_tag="PKJ16_11500"
     CDS             complement(590..1474)
                     /locus_tag="PKJ16_11500"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HNV47657.1"
                     /translation="MVGFLRGMRYFDILILLAFPTMGAVFAIDAWSVPIALRFVVFCI
                     CNAFFLAHVFSFNDISGAHLHPDEPEIRARHALKNLDAKNRAPLFFSIVLLAASLVGY
                     ALLDLVILFFASAIAVLCALYSHHHINLKGVPVASALILLSCPLLYFYTGWIAFAPFT
                     AKTAMMGIFFATVLLAGHFSNEIQDFEQDRCASITTNAIRFGKLTVFRFGLVLFVASS
                     AFLAYVSYAFFDGIRYFIISLILLGSWLAVSSAFWRTPQSIESNIATFRSIYRVIYAA
                     YCLAVFVIRGIDWISIAR"
     gene            complement(1515..3254)
                     /locus_tag="PKJ16_11505"
     CDS             complement(1515..3254)
                     /locus_tag="PKJ16_11505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV47658.1"
                     /translation="MDPATIFDKMLFLDPGAVFTPFRVIVSIALCALLVVAYRFSARP
                     PVAIPTPAPSPKPATRSERLIEWLPIIGVLAVALVIRSINLGINSYNNQEMISLELAN
                     FTASPVKTVLNILSLYTFHQPINTFFVKALSLVTTNEIVQRIPSVVFGTAAVYYGFRL
                     GSYLSSKPTGLLMALVIACAPIAVFYSRSIEAYSLYLMLAILQLYYFIRFADEQDRAL
                     PLVIVSVVGFYTHFFFAVLVAAEIAYAILRHITGSLARNNAVSIYGALALCIAPMTLW
                     LPIVFETFASAERHIDTTVGQIYFPDMHPIRIVLIMVQVFTGIENPIGATLLSAAAVM
                     CCAYLLIIKKRTHQLLFLIITAFGVLFAFTSQVVSAHKIGGVFPITFRHHIYVFVSLF
                     VPLLTLPDAIQPARIRQIALCALAGGAILLGAVSIHQVTRMQHPDYRSLAHDIRRDYD
                     PRTDALAIANTLYIPSIRYYLHTDRSDYRFYNINFMSGFYISGIATDGPKAHEYRFLK
                     PSRTTLPRVIEDHRIKRVWFVIVEERALGVLSYTQQLEYNLLTDLTELFPSHATIKNY
                     FSPDIKTAILQLQ"
     gene            3434..4105
                     /locus_tag="PKJ16_11510"
     CDS             3434..4105
                     /locus_tag="PKJ16_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HNV47659.1"
                     /translation="MIWGIARESAHADVAIATFTTSVADQDANVKTNIARACRTLDGA
                     VIAPEAVFSFSDTVGEASVQNGYVRGRVLYRDEIVYETGGGLCQVSTTLYNAFLIAGF
                     AIVERHRHFQPVSYAPLGLDATIKYGKKDLRMRNTTGQRVRISATMNDRSLVISLSAL
                     APITFRYEIDTEEEELEIPLGQDDRRIRKGISVLVYRKKFLGSRQTESFLLYRDYFPP
                     VYLRR"
     gene            4124..4756
                     /locus_tag="PKJ16_11515"
     CDS             4124..4756
                     /locus_tag="PKJ16_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HNV47660.1"
                     /translation="MNPHANARRIVIALLAVSFASIAIFAHARDGDALDAWARYARSA
                     EAARLLAWVRCHMREEVAGLRCSSPGPGPLPEFRGRLGVFITITTKKTVRGCYGAFDH
                     AGDDIASLLREYLRGALFSDPRTSPLGPGEIDAARIIVTVADRPVAIRDINMLDITRY
                     GVTITDESDRTLVFVPAEIKSVAHVQRRIAGMRIAHIAAFRAITIRERSR"
     gene            4768..9636
                     /locus_tag="PKJ16_11520"
     CDS             4768..9636
                     /locus_tag="PKJ16_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HNV47661.1"
                     /translation="MKNWLVWIGLMIAVVGAVSCSSVERIVRGDGTSAYEKTDRAALE
                     KKDAIVGLVRDSVTGKGVPNAIVEIKNANMGVGYYKVTTDSGGRFRVDDFIKNIRYAI
                     EISAAGYVTFTSTEKIVPGEYTLDLQREAVLAGTVRDSAGAPIEGVEVKVRGMSGDGG
                     RPTMRTATTGRDGSYRFDKLVESGYAVTFEKAGLITETASIQQIKRGETFSLPMLLYR
                     PASLAGRITIKGVQAPAINMEVTLKGKAGHGTNSFQDGSYLIQDVKPGYYKLEISHRG
                     FYNLAPETIRIAEGARIAGKDFVVTPKPPKVAVQAYRYTFSPGTKVEFNLRAFRLEKV
                     RARIYSVPLATLMSGRVDPVKADPKMMGFTTVSEWDDSIKNFEPYEWRYQTIEPAKPL
                     PTGAYCIEVRGAGNTYDRKFFTVTSVGLVVKRSRDSIFAYVSDLVTNRPIKDAKIVIF
                     DNTPDKNEHRTSTTPYKPPERIEKLPVIVVNRGVTSESGVYQFSRRTTQHLSVLAVGP
                     DRSFAFCSTGHPLTFDGEMNKFFIYTDRPVYRAGDTVHYKIVAKKRGTRFTPIANRKV
                     QFEISNSEFEEKLDDGELALDEWGTAHGKLELERDCRLGEIAIRVGETKENLYGAGRF
                     YIEQYRKPEFKIDITPSREYFVNGDTAEFRVEAKYFFGAPLKGALVRYRFYERRLRDT
                     DTRYWWEEDDGASEGYYNRIKLEGEKYVDENGVAVMRLDCGTFPFDREVSLEVTAVDK
                     SNVSITSSSRVRVGRGDYYIKINPKKNFFADNESKRVEVRTVAQDGKPVSAAVRVKLF
                     NYIWKPWQRVYVHAPRPVFEASVTTNATGIATVDLPGKFDVYGEFDFVAEGRDRRENA
                     ITASRVLWVYSARGAKIESRFRNLELAVNENRLEKAGAITCLLKSRFTDAYVCLTVEG
                     RDIYASKVVKMDGNVMPVTIDIKPQYAPNLFISATMQRSRALFTATTEITLPNADTGL
                     SIALETDKKEYLPGEKARVRLKVTDERGAPLAADVSLGTVDESIYYIRSDHTPKMADF
                     FYSKISNWVLTNYSYPITLLAGAGKDDKVKVREKFADTAYWNPRVRTGPDGTAALEFT
                     LPDNLTTWRITARGHDRTGRVGESVFKFLSTQDLIARIGKPRFLVEGDRLSLIGIVNS
                     NTTRGLAKVDTEFTVDGAAVRPDAPVSISLPPYGTARSYYPVTVPEGKERVQVFMKAV
                     ADPKARDALSIKIPVESRGAPFKVYSAGDMAANTAVTIAPVGDTDDFSFRPSDLTISV
                     NAGPIMQMVKATTFLSEYPYGCIEQTLNRFMPVLVLKNLLKHKGVSHMMEDVDIDEKV
                     RAGIARIQGYQNDDGTWGWWSGGRGNEFVTGYVLYSLNRARALGFSSDVNDETVKKGI
                     DAIGTMLSSTQGVPDDAKAYLLYVSALWGRWQEKAFSDLMELRQRNAYQLAFLIRAIR
                     TAKSNVPRKDIGEKTHAAMERVLPEYVNALKQLQKRDARGVFWASEGAQRWGWQGGDT
                     EMSSHVLAALVSVGDRSPVASQLVSSISKRGRNDAWLSTKETASVFLAFCDYLAAAGN
                     GVSGRGDIAFAVEGKKVADVSFDLSKRVRVKDLVRKIKLSDLGAKKSYQVVATGTAGS
                     DVSFECTMAGNL"
BASE COUNT         2106 a         2648 c         3242 g         1814 t
ORIGIN      
        1 tggcgtggcc gtggtgaccc ccgcgccgct gggccagacc tgcgacgaga acgggtgcca
       61 tccgtgcacg catgacgagc ggtattcgaa cctcgtgacg aacctcacgg cgaatttgcc
      121 gaagttactc aggaagttcc agctgacgcc tgaaaatgac ggcgacgcat acatgcagta
      181 cggagagacg atgtacgacg agctcacgca acttggtctc atcgcattta gcccgaacgg
      241 cgtggggacg tatctgctcg acaaccttaa gccggcgtcg cagtacacgg catggaatat
      301 gaccgaggag atcgattcgc tgctcaagaa ggaaatcttc acgacaagca atgaggggac
      361 cttctggttc gagctggggc atttgctgca gtcctttggg gtgattgtca agcagcgcga
      421 aatcaacaac gggcagttgc ttgattacta ttcctcattc aagggcatgt tcgagtaaac
      481 atcacaacac atcgattggt gccgaacggc ggggtgaatc aaccccgccg ttcgttttat
      541 atggaagatg caatagcgat cttttgcgcg gagggatgcg ataatcgtat tagcgagcga
      601 ttgatatcca atcaatgccg cgaatcacga ataccgccag gcagtacgcg gcgtagatca
      661 ctcgatatat tgatcggaag gtggcgatat tcgactcgat cgattgcggg gtgcgccaga
      721 aggcagatga aaccgccagc cacgatccaa gcaggatgag cgaaatgatg aagtagcgaa
      781 tgccatcaaa aaatgcatac gacacatagg cgagaaatgc ggaagatgct acaaacagga
      841 caaggccgaa tcgaaagacg gtgagttttc cgaatcgaat ggcgttggtc gtgatcgatg
      901 cacaccggtc ctgttcgaag tcctggatct cgttcgaaaa atgccccgcg aggagtacgg
      961 tggcaaaaaa aatacccatc atggcggttt tcgccgtaaa tggcgcgaag gcgatccagc
     1021 cggtgtaaaa atacaggagc gggcatgaaa ggagtatcaa tgcactggcg actggaacgc
     1081 ctttgaggtt gatgtggtga tgcgaataga gcgcgcagag cacagcgatg gcgcttgcga
     1141 agaacaggat gacgagatca agaagcgcat atccgacgag ggatgccgca agaaggacga
     1201 ttgaaaagaa gagcggtgcg cggttctttg catccaggtt ttttagcgca tgacgagcgc
     1261 ggatctccgg ttcgtccggg tggagatggg cgccgctgat gtcattgaat gaaaaaacat
     1321 gggcgagaaa gaatgcattg cagatgcaaa atacaacgaa gcgaagagcg atcgggacgg
     1381 accatgcgtc gatggcgaac accgccccca tggtggggaa ggcgagaagg atgagtatgt
     1441 cgaaatagcg catgccgcgg agaaagccga ccatgacgtg taacgtgttt ttcccgcgga
     1501 tcatgggtgc gtgttcattg aagttggaga atcgcggtct ttatgtccgg tgagaaatag
     1561 ttttttatcg ttgcatgcga tgggaaaagt tcggtgaggt ccgtaaggag attgtactcc
     1621 agctgttgcg tgtacgaaag aacgcccaga gcgcgctcct cgacaatgac gaaccagacg
     1681 cgcttgattc tatggtcttc gatgacacgc gggagggtgg ttcgcgacgg tttgaggaag
     1741 cgatactcat gcgctttcgg accgtcggtg gcgattccgc tgatgtagaa cccgctcatg
     1801 aaattgatgt tgtaaaacct ataatcggat cgatcggtgt gcaggtaata gcggatcgat
     1861 gggatgtaga gcgtgttcgc gatcgcaagg gcgtcggtgc gtggatcata gtcgcggcgt
     1921 atgtcgtgtg caagcgatcg gtaatcggga tgttgcattc gggtgacctg gtggatggag
     1981 accgctccga ggaggatcgc gccacctgca agggcgcaca gcgcgatctg acggattcgc
     2041 gctggttgaa tggcatcggg caacgtaagg agcggaacga agagcgacac aaacacgtaa
     2101 atgtgatgac ggaaggtgat tgggaaaacc ccgccgattt tatgggccga aacaacctgt
     2161 gaggtgaacg cgaagaggac gccgaacgcc gttataatta aaaatagaag ctggtgtgtc
     2221 cttttcttta ttatgaggag atatgcacag cacatcacgg ctgcagctga tagcagtgtt
     2281 gccccgatag gattttcgat cccggtgaat acctgtacca tgatgagtac gatcctgatc
     2341 ggatgcatgt cggggaaata aatctggccg accgtggtgt cgatgtgccg ttccgcagag
     2401 gcgaatgtct cgaaaacgat gggaagccac agcgtcatcg gggcgatgca aagggcgagc
     2461 gcgccgtata tactgacggc gttgtttcgc gcaagcgatc cggtgatatg gcgcagaatg
     2521 gcgtatgcga tttcggcggc gacgaggacc gcgaagaaga aatgggtgta aaatccaaca
     2581 accgaaacaa tgaccagcgg aagtgcgcgg tcctgctcgt ccgcgaatcg aatgaaatag
     2641 tatagttgca gtatggcaag catgagatac agcgagtagg cctcgatgct tcgcgagtag
     2701 aagacggcga tcggagcgca ggcgataacg agggccatga gaaggccggt tggttttgac
     2761 gaaaggtatg atccaaggcg gaagccgtaa tatacggccg cggtcccgaa gacgaccgat
     2821 ggaatgcgct gaactatctc gttggtggta acgagcgaaa gcgctttgac gaaaaacgta
     2881 ttaattggtt gatggaatgt gtacagcgac agtatgttga gcacggtttt cacggggctt
     2941 gctgtgaagt tggccagctc gagggagatc atctcctggt tgttgtaaga attgatgcca
     3001 agattgatcg agcgaatgac gagcgcgact gcgagcacgc caatgatggg aagccactcg
     3061 atgagccgtt cggatcgcgt ggccggtttg ggactcggag cgggcgtcgg tatggcgaca
     3121 ggcgggcggg cggagaaacg gtacgcaacg acaagcagcg cgcagagcgc gatcgatacg
     3181 atcaccctga acggcgtgaa caccgcgccc ggatcgagaa acagcatttt atcgaatatg
     3241 gtggctgggt ccattgccag aaagaaacat ccgaatgacg ttcgatgcgc gtgcgccctc
     3301 tgccggttgg aagcgtatcc gcacgcaact tctcttcgag atgaatgtag gatggatcat
     3361 gcgaatgggt aaatcaagga ctttttttat cgacacgaag cattgggcgg ttttttttct
     3421 cataagtttt gggatgatat ggggaatcgc acgggaatcc gcccacgccg atgtcgccat
     3481 cgcgaccttc acgacctccg tggccgacca ggacgcaaac gtaaagacga acatcgcgcg
     3541 cgcctgccgc acgctcgacg gtgcggtgat agcgcccgag gcggtattct cgtttagcga
     3601 tacggtcggg gaggcgtcgg tgcaaaacgg ctatgtgcgc gggcgggtgc tctatcgcga
     3661 cgagattgtc tatgagacgg gtggcggcct gtgccaggtg tcgacgacgc tgtacaatgc
     3721 cttcctgatc gcgggattcg ccatcgtcga gcgccatcgc cacttccagc cggtctcgta
     3781 tgcgccgctg ggcctggacg cgaccatcaa gtacggtaaa aaggacctgc ggatgcgcaa
     3841 cacgaccggg cagcgcgtgc gcattagcgc aaccatgaac gaccgctcgc tggtgatctc
     3901 cctctcggcg ctggcaccga tcacgttccg ctacgagatc gacaccgagg aggaggagct
     3961 tgagattccg ctgggccagg acgatcgacg gatccgtaag gggatatccg ttctggtgta
     4021 ccggaagaag ttcctcggtt cccgccagac ggaatccttc ctgttgtatc gcgactattt
     4081 tccgccggtg tatcttcggc gctgagtatc gcgtggttat ccgatgaatc cgcacgcaaa
     4141 cgctcgtcgt atcgtgatcg cgctcctggc ggtgagcttc gcctcgatcg ccatcttcgc
     4201 gcacgcgcgc gacggggacg cgcttgacgc atgggcccgc tatgcgcgct cggcggaggc
     4261 cgcgcgactg ctggcgtggg tgcgttgcca catgcgcgag gaggttgcgg ggctgcggtg
     4321 ctcctccccg gggcccggcc cccttcccga attccgcggc cggctcgggg tattcatcac
     4381 tatcaccacg aaaaaaacgg tgcggggctg ctatggcgct ttcgaccatg cgggggatga
     4441 tattgcatcg ctgttgcgcg aatacctgcg gggcgcgctc ttttccgatc cgcgcacctc
     4501 accgctgggg cccggcgaga tcgacgcggc gcggatcatc gtgaccgtgg ccgaccgccc
     4561 cgtggccatt cgcgacatta atatgcttga tattacgcgc tacggtgtta cgattaccga
     4621 cgaaagcgac cggacgctcg tgttcgtgcc ggccgagatc aagagcgtcg cccatgtgca
     4681 gcgacggatc gccggcatgc gcatcgcgca catcgcggcg tttcgggcga tcaccattcg
     4741 ggagcgttcc cgctaacgga gaggggtatg aaaaactggt tggtttggat tgggctcatg
     4801 attgcggtcg tgggcgcggt gtcgtgctcg tcggtggagc gtatcgtccg cggcgacggg
     4861 acctcggcgt atgagaagac ggaccgggcg gcgctcgaga agaaggacgc catcgtgggc
     4921 ctggtgcgcg actcggtgac cgggaagggc gtcccgaacg cgatcgtgga gatcaagaac
     4981 gccaacatgg gggtggggta ctataaggtg accaccgact ccggcggacg gtttcgagtc
     5041 gatgatttca taaaaaatat tcgctacgcc atcgagatat ccgccgcggg gtatgtgacc
     5101 tttacgtcga ccgaaaagat cgttccgggc gaatacacgc tggacctgca gcgggaggcg
     5161 gtgcttgcgg gcacggtgcg cgactccgcg ggcgcgccca tcgaaggcgt cgaggtgaag
     5221 gtgcgcggca tgagcggcga cggggggcgg ccgacgatgc gcactgcgac gacgggacgg
     5281 gacggcagct atcgattcga caagctggtg gagtccggct atgcggtcac cttcgagaag
     5341 gcggggctca ttaccgagac cgcttccatc cagcagatca agcggggcga gacgttctcg
     5401 ctgcccatgt tgctttaccg tcccgcttcg ctcgcgggac ggatcaccat caagggggtg
     5461 caggcgcccg ccatcaacat ggaggtgacg ctcaagggca aggccgggca cgggaccaac
     5521 tcctttcagg acgggtcgta tctgatccag gacgtgaagc cgggatatta caagctggag
     5581 atctcgcacc gcggctttta caatctcgcg cccgagacga ttcgcatcgc cgagggcgcg
     5641 cggatcgcgg gaaaggactt tgtggtgacg ccgaagccgc ccaaggtcgc ggtgcaggcg
     5701 taccgctata ccttctcgcc cggcaccaag gtcgagttta acctgcgcgc cttccgcctc
     5761 gaaaaggtcc gcgcgcgcat ctattcggtg ccgcttgcga cgctcatgtc cggccgtgtt
     5821 gatcccgtaa aggccgaccc gaagatgatg ggctttacga ccgtgagcga atgggacgac
     5881 tccatcaaga acttcgagcc gtacgaatgg cgttatcaga ccattgagcc ggcgaagccg
     5941 ctcccgaccg gcgcctactg catcgaggtg cgcggcgcgg gtaacaccta cgaccgaaag
     6001 ttcttcaccg tgacctcggt gggactggtg gtgaagcggt cgcgcgacag catcttcgcg
     6061 tatgtctcgg atctggtgac gaaccgaccg atcaaggacg cgaagatcgt gatcttcgac
     6121 aatacgccgg acaagaacga gcaccggacg tcgacgacgc cctacaagcc gccggagcgg
     6181 atcgagaagc tgccggtgat cgtcgtgaac cggggcgtga ccagcgagag cggggtgtac
     6241 cagttctcgc ggcggaccac ccagcatctg tcggtgctcg cggtggggcc cgaccggagt
     6301 ttcgcgttct gcagcacggg gcacccgctc accttcgacg gcgagatgaa caagttcttc
     6361 atttatacgg accggccggt gtaccgggcc ggggacacgg tgcattataa aatcgtcgca
     6421 aagaagcggg ggacgcgctt cacgcccatc gcaaaccgga aggtccagtt cgagatatcc
     6481 aacagcgagt tcgaggagaa gctggacgac ggcgagctcg cgctcgacga atgggggacc
     6541 gcgcacggga agctcgagct ggagcgcgat tgccgcctgg gtgaaatcgc cattcgcgtg
     6601 ggggagacga aagagaacct ctacggcgcg gggcgtttct acatcgagca gtatcgcaag
     6661 ccggagttca agatcgacat tacgccgtcg cgcgagtatt tcgtgaacgg ggataccgcg
     6721 gaattccgcg tcgaggcgaa gtactttttc ggcgcgccgc tcaagggggc gctggtgcgg
     6781 taccgctttt acgagcggcg gctccgggac accgacacgc gctactggtg ggaggaggac
     6841 gacggcgcct ccgagggcta ttacaatagg atcaagctcg agggcgaaaa atacgtcgac
     6901 gagaacggcg tcgcggtgat gcgcctcgat tgcgggacct tcccgttcga ccgcgaggtg
     6961 tcgctcgagg tgaccgccgt ggacaagtcg aacgtgagca tcacgtccag ctcgcgcgtc
     7021 cgcgtggggc gcggcgatta ctacatcaag atcaatccga agaagaactt tttcgcggac
     7081 aacgaatcga agcgggtgga ggtgcggacg gtcgcgcagg acggaaagcc cgtgagcgcc
     7141 gccgtgcgcg tgaagctgtt caattacatc tggaagccct ggcagcgcgt gtacgtgcac
     7201 gcgccgcgtc cggtcttcga ggcgtccgtg acgaccaacg ccacggggat cgccacggtt
     7261 gacctgcccg ggaagttcga cgtgtacggc gagttcgact tcgtggccga gggacgggac
     7321 cggcgcgaga acgcgattac ggcctcgcgc gtcctgtggg tctacagcgc ccggggcgcg
     7381 aagatcgaat cgcgcttccg caacctggag ctcgcggtga acgagaaccg gctggaaaag
     7441 gccggggcca tcacctgcct cctgaagagc cgcttcacgg acgcctatgt gtgtctgacc
     7501 gtcgaggggc gcgacattta cgcgagcaag gtggtgaaga tggacggcaa cgtgatgccg
     7561 gtgaccatcg acatcaagcc gcagtatgcg ccgaacctct tcattagcgc gaccatgcag
     7621 cggagccggg cgctgttcac cgcgacaacc gagattacgc ttcccaacgc cgacacgggc
     7681 ctgtcgatcg cgcttgagac cgacaagaag gaatacctgc cgggcgagaa ggcgcgggtc
     7741 cggttgaagg tgaccgacga acggggcgcg ccgcttgcgg cggacgtgtc gctgggaacg
     7801 gtggacgaat cgatctacta catccgcagc gaccacaccc cgaagatggc cgatttcttc
     7861 tactcgaaga tctcgaactg ggtgctcacc aactattcct atcccattac cctgctggcc
     7921 ggcgcgggca aggacgacaa ggtgaaggtg cgcgagaagt tcgcggacac cgcctactgg
     7981 aacccgaggg tgcgcaccgg gccggacggc accgcggcgc tcgagtttac gctgccggac
     8041 aaccttacca cgtggcgcat aacggcgcgc gggcacgacc gcacggggcg cgtgggcgag
     8101 agcgtgttca agttcctctc gacgcaggac ctgatcgcgc ggatcggcaa gccgcgcttc
     8161 ctggtcgagg gcgaccgctt gagcctcatt ggcatcgtga acagcaatac cacgcgcggt
     8221 ctcgcaaagg ttgacacgga gtttaccgtg gacggcgcgg cggtgcgacc ggacgcgccg
     8281 gtgagcatca gcctcccgcc gtacggaacg gcgcgctcct attatcccgt aaccgtcccc
     8341 gaggggaagg agcgggtgca ggtgttcatg aaggccgttg ccgacccgaa ggcgcgcgat
     8401 gcgctctcca ttaaaattcc cgtggagagc cgtggcgcgc ccttcaaggt gtactcggcg
     8461 ggcgacatgg cggcgaacac ggcggtcacg atcgccccgg tgggcgacac cgatgatttt
     8521 tcgttccgac cgagcgacct tacgatttcc gtgaacgccg gtcccatcat gcagatggtg
     8581 aaggcgacca cgtttctctc cgagtatccc tacggctgca tcgagcagac gctcaaccgg
     8641 ttcatgccgg tgctggtgct caagaacctc ctgaagcaca agggcgtttc acacatgatg
     8701 gaggatgttg acattgacga gaaggtccgc gccggcatcg cgcgcatcca gggctaccag
     8761 aacgacgacg ggacctgggg atggtggagc ggcggtcggg gcaacgaatt cgtgaccggc
     8821 tacgtgctct attcgctgaa cagagcgcgc gcgctggggt tttcttccga cgtaaacgac
     8881 gagaccgtga agaaggggat cgacgcgatc gggacgatgc tatcgagcac gcagggcgtt
     8941 cccgacgacg cgaaggccta tctgctctac gtgtcggcgc tgtggggacg ttggcaggaa
     9001 aaggcgtttt ccgacctgat ggagttgcgg cagcgcaacg cctatcagct cgcttttctt
     9061 atcagggcca ttcggaccgc gaaatcgaac gtgccgcgga aggacatcgg cgagaaaacg
     9121 catgccgcga tggagcgcgt ccttccggaa tacgtaaacg cgctcaagca gctgcagaag
     9181 cgggacgcgc gtggcgtctt ctgggcgagc gagggggcgc agcgctgggg ctggcagggc
     9241 ggcgataccg agatgtcgtc gcatgtgctc gccgcactcg tgagcgtcgg ggaccgctcg
     9301 ccggtcgcga gccagctggt gagctccatc tcgaagcgcg gccgcaacga tgcgtggctc
     9361 tccacgaagg agacggcctc ggtctttctc gcgttttgcg attatcttgc cgccgccggg
     9421 aacggggttt ccggcagggg cgatatcgcc ttcgccgtgg aggggaaaaa ggtggccgac
     9481 gtctcgttcg atctctcgaa gcgcgtgcgg gtgaaggacc tcgtgcgtaa aatcaagctt
     9541 tccgatctcg gggcgaagaa gagctaccag gtggtggcga cggggacggc gggaagcgac
     9601 gtgagcttcg aatgcaccat ggccgggaac ctgtaattca aggatgcggg gcttctgtcc
     9661 tttctgaaat cccccgagcg cagcctgaac gcgctttcca acgggattgg gctggtgcgc
     9721 cgcttcaatt ccgtgacgcg cgtgcgcgac atgaacaaca gcgagtacct cgtcccgcag
     9781 gacatggcgg gcgaccggaa gatctccgtg
//
LOCUS       DAORHF010000133         9802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6266_length_9802_cov_4.811840, whole genome shotgun sequence.
ACCESSION   DAORHF010000133 DAORHF010000000
VERSION     DAORHF010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9802
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6266_length_9802_cov_4.811840"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="PKJ16_11525"
     CDS             <1..837
                     /locus_tag="PKJ16_11525"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HNV47662.1"
                     /translation="VPEFAPGDEPALKEYAPEQHFTSPPPRYNDASLVKFLEESGIGR
                     PSTYAPTINTLIKRYYVTRSGRQLVPTVLGRLINTIMSKHFPTLVSIDFTANMEEKLD
                     LIEESKSDWKSMLRSFHGPFKEVVDTAADAIEEMKSILDEPTDLICEKCGRNMVKKLG
                     KYGFFLACPGFPECRNSKTLPLGKCPVDGCDGDIVKKSSKKGRAFYGCSKYPACVFIT
                     NDEPSDKHCPKCGGLLFKKRVKGKGEMLTCQNVECDYRVELLDDATAAKNGDENDAAA
                     EA"
     gene            840..1796
                     /locus_tag="PKJ16_11530"
     CDS             840..1796
                     /locus_tag="PKJ16_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNV47663.1"
                     /translation="MFDDIDRFMRYLESEKNASPRTVESYNRDLLQFCSFLSGTLEGA
                     TADAYGIAVPVDGAVPAVSRIVDADIMAFVEYCYDNGLERSSIARKLASIKSFFRFLF
                     NNECIADNPARNVRLPRRDRALPHFLYLNQIDRLSDFPVERFIDFRDRALLSALYSTG
                     ARVTELVTADLVDVDMRAGTLRVSGKGSVQRVVFLADETIDFISSYVAERTRRFGRAD
                     GPLFVNNRGTRITRRGVFHVLLQRAKRCGLLGKVSPHVFRHSFATELLNRGADIRAVQ
                     EMLGHRNLSTTQMYTHTTKERLRAAYDRFHPHAEGRHGKHEQ"
     gene            1786..2340
                     /gene="hslV"
                     /locus_tag="PKJ16_11535"
     CDS             1786..2340
                     /gene="hslV"
                     /locus_tag="PKJ16_11535"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HNV47664.1"
                     /translation="MNSDGTSRSAFRATTVLAVRHSGGFAIGCDGQVTIGDAVIKSTA
                     VKVRRIRGRDIIAGFAGSVADALTLFEKLEMKLETYSGNVPRAVVELARDWRMDRVLR
                     RLEAVLVVGDRESLFLISGTGEVIEPDDGIVGIGSGGQYATAAARALVRTTALAPREI
                     VAQSLAIAAEICIYTNANITIEEL"
     gene            2343..3767
                     /gene="hslU"
                     /locus_tag="PKJ16_11540"
     CDS             2343..3767
                     /gene="hslU"
                     /locus_tag="PKJ16_11540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HNV47665.1"
                     /translation="MPKSEAHKRTARGLTPRETVAELDTYIIGQYAAKKSVAIALRNR
                     ARRQQLDPEMQDEIAPKNIIMIGPTGVGKTEIARRLSRLVNAPFVKVEITKYTEVGYV
                     GRDVESMIRDLCAIAVNMVKEESGALVEEKARQNAEDRIVELLTTAAGAQIAEIREQG
                     FTFGAADEAERAQAAEAALVDARERCRADLRAGALDEEQIEYEAEAGARMPVMSVFSG
                     SGMEEMDFLQNMLGEVMPKRPKKSKLTVVEAKKLFFDEEMDKLIDMDAVRIEAVRRVE
                     EMGIVFIDEIDKIGVGDSRGGGPDVSRQGVQRDLLPIVEGTTVNTRYGPVRTNHILFI
                     AAGAFNIARPSDLIPELQGRFPIRVELDSLTEDDFRKILTLPRNSLTRQYTALLGTEG
                     VRLGFTDEAVDRIAHIAFTVNAQNENIGARRLHTIMEKLLEDILFDAPDLPETERDIV
                     IDAERVEQRLADTLKDRDLTRYIL"
     gene            3969..4859
                     /locus_tag="PKJ16_11545"
     CDS             3969..4859
                     /locus_tag="PKJ16_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47666.1"
                     /translation="MRMHLDERTEGWVGIPHGGIGMGSIMELASFLEAGPRDTNTRYP
                     MSAEFRMGGASVRTGDTVTVSVVSSDGGFTGSIVPDGEDRPYIEGTVRYGADERNRRD
                     DFISFLPASYSEIHESLARLPHYRNCFVCGVERTSPGLNRVFNYLNDSRINKTVVSHV
                     GMDDADDATVHRFSTGSMLHPIAPLALLDEVMGWGGFLVSGQGGVSVRLGYTFYRGIR
                     IGERVVMFGRGTRVKGSVSSRMLFWADGGAAVIGSDGTLEPVIVSSGQWFAVSELTEQ
                     MKIQLMPQDLIDRAFALAGS"
     gene            5064..5957
                     /locus_tag="PKJ16_11550"
     CDS             5064..5957
                     /locus_tag="PKJ16_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47667.1"
                     /translation="MVKRSILLAVILMFVSASIVYAGGSSSSTKCKTKYPIVLSHGMG
                     AQYKIAGGLALYWNDIPDELKENGARVYITSVNSLDSTANKAAAWKKQVLEILAVSGA
                     QKINLIGHSHGTIYSRYAISNLGMAPYIASHTSIAGPHRGSIIAKLVVDVLGGKDNLA
                     YDVLEFLVSLLMGDKSPNAVANMLNLTPDFMKNVFNPATPNKSGIYYQSWAYKILTPV
                     GAGLFLATWPIQKYYEGDNDGLVAVDSAKWGTFRGVITGSWWAGVNHLAAVDMLWGIT
                     PGFNAPAHFVDIVGDLKNKGY"
     gene            complement(6125..7012)
                     /gene="hslO"
                     /locus_tag="PKJ16_11555"
     CDS             complement(6125..7012)
                     /gene="hslO"
                     /locus_tag="PKJ16_11555"
                     /inference="COORDINATES: protein motif:HMM:NF001033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HNV47668.1"
                     /translation="MDTISRFHIRDLRLRAYTSITTDTVRSLTDIHATTPSATRAFGR
                     AITATALLGATLKPDSAQTITLKFSGSGPIREVHVQSDARGNIRGYVSNPAIDGAGEI
                     RLIEFSRLIGAGMLTITKDIGLREPYTSVMPLLHGDISRDLASYLATSEQTPSALIIG
                     LAMNSDGSISASGGILVQTFPDTTPDVVERVETSIAGAAQPLGEFLRGGGAISHYLSG
                     LLGGAAAELLAETTLSAACRCNRALIGAILQSLGEDELRDMIEKDGGAELACLFCRQK
                     YHFSADDLRAIVTNSAVSN"
     gene            7237..8136
                     /locus_tag="PKJ16_11560"
     CDS             7237..8136
                     /locus_tag="PKJ16_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HNV47669.1"
                     /translation="MKKLRMIALLMCVGYVMMAFVANHANAGGKKASLNLKYPIILAH
                     GYAGSDEFLGCIDYFYGIKNELKDEGADVYAPDLGAFDTSAKRGEKLKRFVLEVLAAT
                     RKPKVNIIGHSQGGVTSRYMISNMGMSGKVASLVMISSPNRGTSLADFVIFKLPNVAG
                     KAITGIANSLWGGLLCADRNPNFYAATYELTRHNMTKNFNPNTPDKTGIRYYSYAGKM
                     YGVSANIILTPTWALIRYYDGENDGIVPVASSVWGNWKGKVTGLFGVDHFMEINHLFG
                     NTPGFDAEGFYVKIAKMLQSDGC"
     gene            8311..>9802
                     /locus_tag="PKJ16_11565"
     CDS             8311..>9802
                     /locus_tag="PKJ16_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV47670.1"
                     /translation="MDDILGKINIFIVPPVLALAIGLTLAVLSLVKGRVGGRFRTENV
                     LFALTCIWWSSLLAPAFICHQLFRGDIDFILRIERSIHFFYVYIPLVNLLYFHQIFQV
                     QRRWLVIVCAVMSCAISVSTQTEYYFTGMHTYSWGYIAKGGPAFDAYGLYGTSIVVYI
                     IVLSFRKIRAETNPVARLKMKFITLSLMTSAFLTMMNVPAINGIDFYPMGNFNFVALG
                     ILAYGVLRYRFMNVRSVLHITLIWAVLSSLIFIPNYLLFRWVKPRVIDLPDAPLFAIL
                     IAWFFLNIAYIRKVQPMIDQLFNRRKFDLIAEEHEFIAEIAQLQTLSALTGRLIDVMK
                     KALVFAEAEYVVPHHEGTGDGGSGAHRAAVPAEMSAWLVRHNHLIERTMAETDPEYAD
                     MRGPLLALFESLRCEYVIPFVHNGELIGLLKLSERRNLRQLDASEVSFINNIRSAASI
                     ALANSTMYQNLSDLKDNLEEKVQERTKELSMARDALWGEMELAKKIQ"
BASE COUNT         2036 a         2789 c         2965 g         2012 t
ORIGIN      
        1 gtccccgagt tcgcgcccgg tgacgagccg gcgctcaagg aatacgcgcc cgagcagcat
       61 ttcacgtcgc ccccgccgcg ctataacgac gcctccctgg tcaagttcct cgaggagtcg
      121 ggcatcgggc gtccgtcgac ctacgcgccc accatcaaca cgcttatcaa gcgctattac
      181 gtgacgcgat cgggccgtca gctcgttccc acggtcctgg ggcggctcat caacaccatc
      241 atgtcgaagc attttcccac cttggtctcc atcgatttca cggcgaacat ggaggagaag
      301 ctcgatctca tcgaagagtc gaaatcggac tggaagagca tgctgcgctc gttccacggg
      361 cccttcaagg aggtggtcga taccgccgcc gacgccatcg aggagatgaa gagcatcctc
      421 gacgagccga ccgatctcat ctgcgaaaaa tgcggccgca acatggtcaa gaagctcggg
      481 aagtacggct tctttctcgc ctgtcccggg ttccccgagt gccgcaattc gaagaccttg
      541 ccgctgggaa agtgccccgt ggacggctgc gacggcgata tcgtgaagaa atcatcgaaa
      601 aaaggccggg cattctacgg gtgctcgaag tatcccgcct gcgtgttcat aaccaatgac
      661 gagccgtccg acaagcactg ccccaagtgc ggcggattgt tgttcaaaaa gcgcgtgaag
      721 ggcaaagggg agatgctgac gtgccagaac gtagagtgcg attaccgcgt ggagctgctg
      781 gacgacgcga ctgcagcgaa aaacggtgac gagaacgacg ccgccgcgga ggcgtgatcg
      841 tgttcgacga catcgatcgc ttcatgcgct atctggagtc cgaaaaaaac gcctcgcccc
      901 ggaccgtcga atcgtacaac cgcgaccttc tgcaattctg ctcatttctc tccggcaccc
      961 tcgagggagc aacggccgac gcctacggga tcgccgtccc tgtcgatggc gccgtacccg
     1021 cggtatcgcg cattgtcgac gccgatatca tggccttcgt cgaatactgc tacgacaatg
     1081 gcctggagcg ctcttccata gcccggaagc tcgcttcgat caaatcgttc ttccgcttcc
     1141 tgttcaataa cgaatgcatt gcggacaacc ccgcgcggaa cgttcgcctt ccgcgccgcg
     1201 accgcgcgct cccgcacttt ctgtacctga accagatcga ccgcctgagc gacttcccgg
     1261 ttgagcgctt catcgacttt cgagaccgcg cgctcctctc ggcattgtat tcgaccggcg
     1321 cccgcgtgac cgaactggtc acggcggacc tcgtcgatgt cgacatgcgg gccggaacgc
     1381 tccgggtgag cggcaagggc tccgtgcagc gcgtcgtgtt tttggccgat gaaacgatcg
     1441 atttcatctc gtcctatgtc gccgagcgca cccgtcggtt cggtcgcgcc gacgggccgc
     1501 tgttcgtgaa caaccgcggg acgcggatca cccggcgggg cgtgttccat gtgctgctgc
     1561 agcgggcgaa gcgctgcggc ctcctgggca aggtgtcgcc gcacgtgttc cgccacagct
     1621 tcgcgaccga gctcctcaac cggggggccg atattcgcgc ggtgcaggag atgctcgggc
     1681 accgcaacct gtcgaccacg cagatgtaca cccacacgac caaggagcgc ctgcgcgccg
     1741 cctacgacag attccatccg cacgcggagg gacgccatgg gaaacatgaa cagtgacggc
     1801 acgtcgcgga gcgcatttcg cgcgaccacg gtgctcgcgg tgcgccattc gggcggattc
     1861 gccatcggct gcgacggcca ggtgacgatc ggcgacgccg tcatcaaatc gaccgcggtg
     1921 aaggtgcggc gcattcgggg acgcgacatc atcgccgggt tcgccggctc ggtggcggac
     1981 gccctcacgc tcttcgagaa gctcgagatg aagctcgaga cctattcggg caatgtgccg
     2041 cgggcggtgg tcgagctcgc ccgcgattgg cggatggacc gggtattgcg ccgactcgaa
     2101 gcggtcctgg tggtcggcga tcgcgaatcg ctctttctta taagcggcac cggcgaggtc
     2161 atcgagcccg atgacggcat cgtgggcatc ggctcgggcg gccagtacgc gaccgccgcc
     2221 gcgcgggcgc tcgtgcgcac caccgcgctt gcgccgcggg agatcgtcgc gcagtcgctc
     2281 gcgatcgcgg cggagatctg catctatacc aatgcgaata tcaccatcga ggagctgtag
     2341 caatgccgaa atccgaggcc cacaaacgga cggcgcgcgg actcacgccc cgtgaaacgg
     2401 tcgcggagct ggacacctat atcatcggcc agtatgcagc caagaagtcc gtcgccatcg
     2461 ccctccgcaa tcgcgcccgt cgccagcagc tcgatcccga gatgcaggac gagatcgccc
     2521 cgaagaacat catcatgatc gggcccaccg gggtggggaa gaccgagatc gcccgcaggc
     2581 tgtcgcgctt ggtgaacgcg ccgttcgtga aggtggagat caccaagtac accgaggtcg
     2641 gctatgtggg acgcgacgtt gagtcgatga tccgcgatct gtgcgccatc gcggtgaaca
     2701 tggtgaagga ggaatcgggt gcgcttgtgg aggagaaggc ccggcagaac gccgaggatc
     2761 gcatcgtgga actcctgacg acggccgccg gcgcgcagat tgcggagata cgagaacagg
     2821 gcttcacctt tggcgccgcc gatgaggccg aacgggcgca ggccgccgag gcggctctcg
     2881 tcgatgcgcg cgagcggtgt agggcggacc ttcgcgcagg agcgctcgac gaggagcaga
     2941 tcgaatacga ggcggaggcc ggcgcgcgca tgccggtcat gtcggtcttc agcggttcgg
     3001 gcatggagga gatggacttc ctgcagaaca tgctcggcga ggtaatgccc aagcggccga
     3061 aaaaatcgaa gctgaccgtc gtcgaggcaa aaaagctttt tttcgacgag gagatggaca
     3121 agctcatcga catggacgcg gtgcgcatcg aggccgtccg tcgcgtcgag gagatgggga
     3181 tcgtgttcat cgacgagatc gacaagatcg gcgtcgggga cagccggggc ggaggtcccg
     3241 acgtgtcgcg tcagggcgtt cagcgcgatc tactgccaat cgtggagggg acaaccgtaa
     3301 acacgcggta cggtcccgtg cggaccaatc atatcttatt catcgcagct ggggcgttca
     3361 atatcgcccg gccctccgat ctcattcccg aactgcaggg gcgatttccc atccgggtgg
     3421 agctggattc gctcaccgag gacgatttca ggaagatcct gacgctcccc cgcaactcgc
     3481 tcacgcgcca gtacaccgcg ctcctgggaa cggagggcgt gcgcctcggc tttaccgacg
     3541 aggccgtcga tcgcatcgcc catatcgcgt ttacggtgaa cgcgcagaat gaaaacatcg
     3601 gcgcccgccg gctgcacacc atcatggaaa agctgctcga ggacatcctg ttcgatgccc
     3661 ccgacctgcc cgagacggaa cgcgacatcg tcatcgacgc cgaacgcgtg gagcaacggc
     3721 ttgccgatac gctcaaagac cgcgacttga cgcgatacat cctgtagccg gtcattcttc
     3781 ggatgcaaaa gcgagcgtga cgaagaagac gccagggcga ttgtcatgaa atatttcaca
     3841 gaggagcgcg aagcggctgg ttcgatagag caaggaatgt gcggaggttg gcaggcatga
     3901 atggcggatt tgtaacggaa tttttattcg gggatactcg ggttgctaca gacagcgaac
     3961 gagcgacggt gcgcatgcat cttgacgagc gcaccgaggg ctgggtgggg ataccccacg
     4021 gcggcattgg catggggtcc attatggagc tcgcctcgtt tctcgaagcc ggtccgcgcg
     4081 atacgaacac ccgctatccc atgtccgccg aattccggat gggcggcgcc tcggtgcgga
     4141 ccggggatac ggtcacggtt tccgtcgtat cgagcgacgg cggtttcaca ggatcgatcg
     4201 ttcccgacgg agaggaccgt ccctatatcg agggaacggt gcgatacggc gctgacgaac
     4261 ggaatcggcg cgacgatttc atttcgtttc tacccgcgtc ctattccgaa atccacgagt
     4321 cgctcgcccg gctcccgcat tatcggaatt gcttcgtatg cggcgtggag cgaacgagcc
     4381 ccggcctcaa ccgcgtgttt aattacctca acgacagccg aataaacaag accgtcgtct
     4441 cgcatgtcgg gatggacgat gctgatgacg cgacggtcca tcgattttcg acgggatcga
     4501 tgcttcatcc catcgccccg ctcgccttgc tcgacgaggt gatggggtgg ggagggttcc
     4561 ttgtgtcggg gcagggaggc gtttcggtgc gtctggggta cacattttat cgggggatcc
     4621 gcatcggcga gcgtgtggtg atgtttgggc gcggaacgcg cgtcaagggc agtgtaagct
     4681 cacgcatgtt gttctgggcc gatggcggcg ccgcggtcat cggttctgac gggacgctgg
     4741 agccggtcat cgtatcgtca ggacaatggt ttgccgtatc ggaactcacc gagcagatga
     4801 aaattcagct aatgccacaa gacctcatag atcgcgcctt cgcgcttgcc ggatcataac
     4861 aaagagaaac aagcataaaa aaagaatcaa tttttcgtac aagctaattg tttctgtaca
     4921 taaaaataga caaaatacac aagaaaatct attttttgat aaaatattaa accgtccaac
     4981 cgttttttaa ttgacataat tcatgttttc cagtactttc atgtcagata aatgataaat
     5041 cgagtgtctg atgaggaggt cacatggtta aaaggtcgat tctgcttgcg gtaattctca
     5101 tgttcgtgtc tgcctcgatc gtctatgcgg gcggcagttc gtcgagcacc aagtgtaaaa
     5161 ccaagtatcc gatagtgctc tcacacggga tgggcgccca gtacaagatc gccggcggtc
     5221 tggctctata ttggaacgat attcccgacg agctcaagga gaacggagcc cgggtgtata
     5281 ttaccagcgt aaattcgctc gattccaccg caaacaaggc cgcggcgtgg aagaagcagg
     5341 tgctggagat cctcgccgta tccggcgcgc aaaaaatcaa cctcatcggg cattcccatg
     5401 ggaccatcta cagccggtat gcgatatcca atctgggcat ggccccgtat atcgcgtccc
     5461 acaccagcat cgcggggccg caccgtggca gcatcatcgc aaagctcgtg gttgatgtgc
     5521 tcggcgggaa agacaatctc gcctatgacg ttctcgaatt ccttgtctcg cttctcatgg
     5581 gcgacaaaag tccgaacgcc gtcgccaaca tgctgaacct gacccctgat ttcatgaaaa
     5641 acgtcttcaa cccggccacc ccgaacaagt ccgggattta ttatcaaagc tgggcgtaca
     5701 agatcctcac gcccgtcggc gcgggtctct tccttgcgac gtggccgatt cagaaatact
     5761 atgagggcga taatgacggt ttggtcgcgg tcgattcggc gaaatggggc acgttccgcg
     5821 gcgtgataac cggatcctgg tgggcgggcg tcaatcacct cgccgcggtc gacatgctct
     5881 gggggatcac tcccggcttc aatgcgccgg cgcattttgt cgatatcgtc ggcgatttaa
     5941 agaacaaggg atactgatcc cggtcatcat cgtatcgcga gcaaacgcga gcctccgata
     6001 ttcgggggct cgtttgtttt ggatgatgcg tttgtttcgg agtgcgcgcc ggtcgatttc
     6061 gaaagaaccg cccggcccag aacgcgctgt tccggttcac gaaatttcaa gctcttccgg
     6121 cggatcaatt cgacacagcg gaattcgtta cgatggcgcg caggtcgtcg gcggaaaaat
     6181 gatatttctg tcggcaaaag aggcatgcca gctcggcgcc tccgtccttc tcgatcatgt
     6241 cgcggagttc gtcctcgcca agcgattgca gtatggcgcc aatcagtgcg cgattgcacc
     6301 tgcacgcggc cgacagcgtc gtttccgcga ggagctccgc ggcggccccg ccgagaagcc
     6361 ccgagagata gtgcgatatc gcaccgccgc ctcgaaggaa ctcccccagc ggttgtgccg
     6421 caccggcgat cgaagtctcc acgcgctcga cgacatccgg cgtcgtgtcg ggaaaggttt
     6481 ggacgaggat gccgcccgag gccgagatcg agccgtcgct gttcatggcg agcccgatga
     6541 tgagcgccga cggcgtttgc tccgaggtgg cgagatacga tgcgaggtcc cgcgagatgt
     6601 ccccgtgcag taggggcatg acgctcgtat agggctccct gaggccgatg tccttcgtga
     6661 tggtgagcat gccggcgccg atgagtcgcg aaaactcgat caatcgtatc tccccggcgc
     6721 cgtcgatcgc gggattggaa acatagccgc gaatattgcc gcgtgcgtcg gattgcacat
     6781 ggacctctcg gatgggcccg cttccggaaa atttcagggt gatggtttgc gccgaatcgg
     6841 gcttgagcgt ggccccgagg agggccgttg cggtgatggc gcgcccaaac gcgcgcgtcg
     6901 cgctgggggt cgttgcgtga atgtcggtga gcgaccgaac cgtgtcggtt gtgatggacg
     6961 tataggcgcg gagtcggagg tcgcgtatgt gaaatctcga tatggtgtcc attgaatgcc
     7021 cgttttggag aatcggtggt atggggtatt ggcgctatca tcgattattt atcgattttg
     7081 tcaagaaatg agcttttggg agggaggttt tatcgaattt cggctgatca atttctgttc
     7141 cgttgttttt tgataagacg atgaagaagt aatttcgcta tacttaacac ataccgaccg
     7201 gatggttttc ggcttggagt attaaagggg ggcgtgatga aaaaactgag aatgattgct
     7261 ctactgatgt gtgtcggata tgtgatgatg gcgtttgtgg cgaaccatgc gaatgcaggc
     7321 gggaaaaagg catcgctcaa cttgaaatac ccgattattc ttgcgcacgg ctatgcgggg
     7381 agcgacgagt tccttggatg tatcgactat ttctacggta ttaaaaatga actgaaggac
     7441 gagggtgccg acgtatacgc tcccgatctt ggcgccttcg atacgtcagc caaacgcggg
     7501 gagaagctga aacgcttcgt gctcgaggtg ctggcggcca cgcggaagcc taaggtgaat
     7561 atcatcggcc actcccaggg gggtgtcact tcgcggtaca tgatatcgaa catggggatg
     7621 agcggaaagg tcgcgtcgtt ggtgatgata tcgtctccga accggggaac ctcgcttgcc
     7681 gattttgtca tcttcaagct gccgaacgtc gcgggaaagg ccatcactgg aatcgcgaac
     7741 agcctttggg gcggactcct ctgcgcggac aggaatccca acttctatgc ggccacctac
     7801 gagctcaccc gtcacaacat gaccaagaac ttcaatccga acacgccgga taaaacaggc
     7861 attcgctact acagctatgc cgggaagatg tacggcgtct ccgccaacat cattctcact
     7921 cccacctggg cgctcatacg ctattacgat ggggagaacg acggcattgt gccggtcgcg
     7981 agctccgtgt ggggaaactg gaagggcaag gtgaccggcc tcttcggcgt tgatcatttc
     8041 atggaaatca atcatctttt cggaaacacg ccgggattcg acgccgaggg tttttatgta
     8101 aaaatcgcga aaatgctaca gagcgacggt tgctaaaccc tccaatatcg caatgcgaac
     8161 catcaggagt ctcgaacatc gaggctcctg agtgtttttg agggatgata atttttctcc
     8221 gaaaacgagg aaagtttttg acgtgtcgat gatcgttgtt tgaataccgc tgtaatgggg
     8281 aaacagcgta catgattacc ggagcacgcc atggatgata ttctcggaaa aatcaacatc
     8341 ttcattgtcc ctccggtgct tgcgttggcg atcggtctta cgctggccgt actctcgctg
     8401 gtaaaagggc gagtgggcgg acggttccgc acagagaacg ttcttttcgc cctgacctgc
     8461 atctggtggt cgtcgctgct cgcgcccgcg ttcatttgcc atcagctctt caggggcgat
     8521 atcgatttta tccttcggat cgagcgaagc atccatttct tttatgtgta tataccgctt
     8581 gtaaatcttc tgtatttcca ccagattttt caggtacagc gtcggtggct ggtcatcgtg
     8641 tgcgcggtga tgagttgtgc gatctccgtc tccacccaga ccgaatacta ttttaccggg
     8701 atgcacacct acagctgggg atacattgcc aagggcggtc cggccttcga cgcctatggt
     8761 ctgtacggga cgtctatcgt cgtatacatc atcgtgttaa gcttccgaaa gatacgggcc
     8821 gagacgaacc cggttgcgcg cctcaagatg aaattcatca cgctctcgct catgaccagc
     8881 gcctttctca ccatgatgaa cgttcccgcc atcaatggga tcgacttcta tccgatgggg
     8941 aacttcaact tcgtcgcgct gggcattctg gcctacggcg tgctgcggta tcgcttcatg
     9001 aatgtgcgca gcgtgctgca tatcacgctc atctgggcgg tgttgtcgtc gctcatcttt
     9061 attccgaact accttctttt ccgatgggtc aagccgcggg tgatcgatct gccggatgcg
     9121 ccgttgttcg caattctcat cgcctggttc ttccttaaca tcgcctatat tcggaaggtg
     9181 cagccgatga tcgaccagct gttcaaccgc cggaagttcg accttatcgc cgaggagcac
     9241 gagttcatcg ccgagatcgc acagctgcag accctctcgg cgctcaccgg caggctcatc
     9301 gatgtgatga agaaggccct cgtcttcgcc gaggccgaat acgtggtccc gcatcacgaa
     9361 ggaacgggcg acggcggctc cggggcgcac agggccgcgg tcccggcgga gatgtccgca
     9421 tggctggtgc ggcacaatca tctcatcgag cggaccatgg ccgaaaccga tccggagtac
     9481 gccgacatgc gcggtccgct gctggcgctg ttcgagtcgc tgaggtgcga gtatgtcata
     9541 ccgttcgtcc acaacggcga gctcatcggc ctgctcaagc tctcggagcg gcgcaacctc
     9601 cgccagctcg acgcgagcga ggtcagcttc atcaacaata tccgttcggc ggcctcgatc
     9661 gccctcgcga actcgacgat gtatcaaaac ctttcggacc tcaaggacaa tctcgaggaa
     9721 aaggttcagg aacgaacgaa ggagctcagc atggcgcgcg acgcgctctg gggcgagatg
     9781 gagctcgcga agaagattca aa
//
LOCUS       DAORHF010000134         9736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6344_length_9736_cov_5.826774, whole genome shotgun sequence.
ACCESSION   DAORHF010000134 DAORHF010000000
VERSION     DAORHF010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9736
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6344_length_9736_cov_5.826774"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1310
                     /locus_tag="PKJ16_11570"
     CDS             168..1310
                     /locus_tag="PKJ16_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47671.1"
                     /translation="MEEKILIIDDDENLLSSFKRSLGRDFTIETAAGGEAGVAAMAKN
                     GPFAVIMSDLKMPGMDGIQVLARARELAPKSVRIMLTGNADLAIAIDAINRGNIFRFL
                     TKPCPPEELAAVLAAATEQYRLVMAEKELLEKTLSGSIKVLTDVLSLVNPRAFSHASR
                     VRRLVRQLASAMNFARTWELEISAMLSQVGCVTIPEDILDKVFALKPLTPPELAMYQN
                     YPKAGSDIIANIPRMARVADIIRNQESHFDNSKPAVGGPAGTKIPPESYILKTALDYD
                     LYIGAGMAADAALIEMHKHASRYDPAIIKALEMVITGEDKFEIRQVGIDQLTCKMILA
                     EDVIANNGKLIVSKGEEVTASMKMRLVNFNKSMGLRDTFRVIVVKD"
     gene            complement(1326..1943)
                     /locus_tag="PKJ16_11575"
     CDS             complement(1326..1943)
                     /locus_tag="PKJ16_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47672.1"
                     /translation="MTTERSICRPGHGASCALCCCSHNYRAPYAEIDALFERRAALLS
                     QFSRGYLIGAVRGSRSDMTGSYYFRGERVMSDVIEKRVENAFQCPFVGYIDGERTVGC
                     VLYPDEHKCGAAFDCHQVYRSKRFTCAARDTLSETEYTYAARLCGDWYHYGALIHMPD
                     FLGALMDEHPDVRLVSFDARRAIFARIEGFILQDDALHRIDSYFG"
     gene            complement(1951..2367)
                     /locus_tag="PKJ16_11580"
     CDS             complement(1951..2367)
                     /locus_tag="PKJ16_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47673.1"
                     /translation="MKKIIVIIGGAREALDEFDFIKQVLGVTLIKASSFSVFSVYISE
                     HHERLGDVSAFIVIGKTGEDRYHANLNAIRVIKNSEEFSPLPILFVGEADPPEFVSHC
                     ENLEVRYLPRPIDQGELIAQLQDILAREAPPSFDED"
     gene            2523..3632
                     /locus_tag="PKJ16_11585"
     CDS             2523..3632
                     /locus_tag="PKJ16_11585"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HNV47674.1"
                     /translation="MKSIDSIVLCLLVSLFWLCHAQAAPPASFHGVFHPCYDESKKKY
                     TVCERGRRVRIDRIMPCAEGVYTNPDHWFDEVRPAMKGRDANGAEYRIVYVGWKYNGE
                     VASCGFEIFRAGTHHSGWHMHFWFVKNDNEWRLLEIVKVPSGRIDFRITPSFGPSGLF
                     PFQYYYPVTATTPPSGFGFVDVNGVVVIKPEFNIAHPFSDGLALVARRPPSKRGRWDR
                     YSREWMYIDVRGATVIAPARYDKPPRSFRSGRALIEKNGYAGFIDTTGKIVIPPDRFD
                     RTNTTDFSEKGIARVSWWHDPAANYCYRRINACHSCLTFRYLAGRIGGSPFIYLKEKI
                     RMYGVIDRNGAVLYSSESSDEVGRWVSKRYYGDES"
     gene            complement(3752..4147)
                     /locus_tag="PKJ16_11590"
     CDS             complement(3752..4147)
                     /locus_tag="PKJ16_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015083549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNV47675.1"
                     /translation="MTGEIQQLLQKARQSLDAATLLLHDRYTDFSAARSYYAMFYALE
                     ALLLSRNQSYSKHSAVIAAFGKDFVKTGLFDDRYHRHVLEAFEIRNIGDYGIINSVNA
                     AKADEMIHKAHELIDAIEAFLRNAHCEER"
     gene            complement(4144..4479)
                     /locus_tag="PKJ16_11595"
     CDS             complement(4144..4479)
                     /locus_tag="PKJ16_11595"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV47676.1"
                     /translation="MKPRIPEQIVPVLNEARKTLLELYTSRLKGMFLYGSYARGDYMD
                     GSDIDVAILLDGAVTPMDELERYLPHISPIALKHDTVVSITVINSDYFKAARTPLILN
                     IIREGIAIQ"
     gene            complement(4612..5475)
                     /locus_tag="PKJ16_11600"
     CDS             complement(4612..5475)
                     /locus_tag="PKJ16_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV47677.1"
                     /translation="MIINRNLHAINLSNILKFKNWDIGKSAERVSSGLRINRAGDDAA
                     GLAVSEKMTGQIHGLRQAERNTEDGISIVNTAEEYLRQSTDIMSRMRSLAVQAANGTY
                     TPADRQLIQVEISALVDEIDRVASQADFNTMKIMLGDFSRHNPHASMWFHVGPNSFQK
                     ERIYIGTMTARGLGIRNDSGALAASLSSPEAANDAIGLLDHAIQRIVKQRSDLGAYIS
                     RFEKTAQELSQAWESAHGASSVVRDADVAEVMVALTKDTIIAETNMAMLAQANVQPNS
                     VIALLREKLPF"
     gene            5651..7573
                     /locus_tag="PKJ16_11605"
     CDS             5651..7573
                     /locus_tag="PKJ16_11605"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV47678.1"
                     /translation="MEGTEKKKILIVEDSATAALATSHVLGQFGYQSIIVDTGEAAVE
                     AVRGDPSLSLVLMDIELGSGIDGAEAARRILAVRALPIVFLTAYAEEHMAERVRGITR
                     YGYVIKDSSEYVLQSAIEMAYELFDAHCRIAESEERYRLLSENTDDVIWLWDLAGNRY
                     EYVGSSVLRLLGFAVDEVMRQSLSDALPAKHAGLLTEELPRRVAALEAGDESARFKTY
                     AVDQYHKDGRLIPTEVSTSLVTDLRGRVTGLQGVTRDISEQRRLTTELAQANRHLAAL
                     WGVAELADAGFKDICDHILRAVVEMTGSGYGFYGFINDDESVMTIHAWSGDAMRDCAL
                     VDKPQDFSIASAGVWAEAVRRREALVINDFAAPHDAKRGYPAGHVALTRLLAVPVFSR
                     GRIVSVAAVANRETPYGPADVSQVGAFLTGIMSVVERRRAEEEVRRLLAEKELLLREV
                     HHRIKNNMSTVISLLDIQSNAPGNEAAGPALEEANRRLRVMMIIYDKLYRSKEYGALS
                     ADRYLEEFVPDMFSGFPHGGRVRLDMAVEPISLDVKFLFPLGIIINELLANSMKYAFP
                     EGRTGTVRIGLRRSDAHAVELVFRDDGIGFAGDVDLASARSFGLQLVSLLVRQINGAV
                     AVNGRDGAEYVITFAA"
     gene            7612..7920
                     /locus_tag="PKJ16_11610"
     CDS             7612..7920
                     /locus_tag="PKJ16_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HNV47679.1"
                     /translation="MANDPLAVIRESDPALFATLGDMRECALGEGALSKKHKLLIALA
                     IDAAIKSENGVRSLARQALEAGASRREIMEALRVVNYICGAGSMYAAAAALADVFPDA
                     "
     gene            7942..8646
                     /locus_tag="PKJ16_11615"
     CDS             7942..8646
                     /locus_tag="PKJ16_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47680.1"
                     /translation="MMSTAFRIIPVVAVLLACLWGSAPPAAAQSKKMTILEYYNSFVM
                     WKKLGFEHLIDRKIGPEGRQEHASRGAYMYNIIVDAPNGFLQYATGCSCHQEINSYAI
                     FKTAGGDVYLSASGRNLNFYKFVGANKWIDETKTVVPETGLGAFLAEGASPASIPANV
                     RKCSAIFYAVPRYGTVVTAELRLYSKHQDDPERSEQGWKRLEALRPAFRKAYRYTKLN
                     LPWDRERGIITIGKKE"
     gene            8659..9585
                     /locus_tag="PKJ16_11620"
     CDS             8659..9585
                     /locus_tag="PKJ16_11620"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV47681.1"
                     /translation="MGDDSDVRAKERPAVLATHTVPEGTDGARLSEYAIGVFEMVPTR
                     NGIKNAIRRGTVSVDGAPGTTATRVRAGQRIDLIDAPGPVRPVYEMKLHVVYEDEHLA
                     VVDKPAGILVSGNAFRTVENALPFNLAASGIRSALARPRPVHRIDAQTSGLLLVAKTS
                     LALVALGRGFEGRDISKRYRAIVIGDIAGPGRIDNHIDGRDAVSEYAPVFSVPSLRSG
                     RLSLVDLFPRTGRTHQLRRHLAHIGHPILGDSLYGPEGLILRGKGLFLAAVEISFAHP
                     VGGAPMRFAIDEPAKFRTLLERERRRWEKFHE"
BASE COUNT         1836 a         2821 c         3123 g         1956 t
ORIGIN      
        1 gcctggccat cgcgcatgcg gcgatcgtca agaaacacgg cggaacactc acgttcgaga
       61 cggtgacagg caggggaact acgttccata ttcaaattcc catcgtggcc gtttcgggcg
      121 tttgacaaca gtagagcatc gttatttatg gaaaagggag gaaatttatg gaagaaaaaa
      181 ttctcattat cgacgacgac gagaacctgc tttcatcctt taagcgttcg ctgggaaggg
      241 atttcacgat cgaaacggcc gccggcgggg aggcgggcgt tgcggccatg gcgaaaaacg
      301 gaccgttcgc ggtcatcatg tcggatctga aaatgccggg catggacggt atccaggtcc
      361 tggcgcgcgc gagggagctg gcccccaaga gcgtacggat catgctcacc ggcaatgccg
      421 atctcgcgat cgcgattgac gcgatcaacc ggggtaacat cttccgtttt ctcaccaagc
      481 cgtgcccgcc ggaggagctg gccgcggtgc tcgcggccgc cacggagcag tatcggctgg
      541 tcatggcgga gaaggagttg ctcgagaaga ccctgagcgg cagcatcaag gtgctcactg
      601 atgtactctc tctggtgaac ccgcgcgcct tcagccacgc ctcgcgggtg cgccgcctgg
      661 tgcgccagct cgcttccgcg atgaacttcg cacgaacctg ggaactcgaa atcagcgcga
      721 tgctctcgca agtgggatgc gtaaccatcc cggaggacat cctcgataag gtatttgccc
      781 tcaagcccct caccccgccg gagttggcga tgtatcagaa ttatccgaaa gcgggaagcg
      841 acattatcgc gaacataccg cgcatggcgc gtgtcgccga tatcatccgg aatcaggaga
      901 gccacttcga taattccaag ccggccgtcg ggggacccgc ggggacgaag atccctcccg
      961 agtcgtacat cctgaagacg gcgcttgatt atgatctcta catcggcgcc gggatggccg
     1021 cggatgctgc gcttatcgag atgcacaagc atgcatcgcg gtacgatcca gcgatcatca
     1081 aggcgctcga gatggtgata accggcgagg ataagttcga gatccggcag gtgggcatcg
     1141 accagcttac ctgtaagatg atcctggccg aggacgtcat cgccaacaat ggcaagctca
     1201 tcgtctcgaa gggcgaggag gtgaccgcat cgatgaagat gcgcctggtc aacttcaata
     1261 aaagcatggg tctccgagat accttccggg tgattgtagt gaaggactga ttcttatcct
     1321 cgccctcacc caaagtacga atcaatccgg tgcagggcgt cgtcctgcag aatgaaaccc
     1381 tcgatgcgcg cgaagatggc gcgccgcgcg tcgaacgaga cgaggcgcac gtcgggatgt
     1441 tcgtccatga gcgcgccgag gaaatcgggc atgtggatga gcgcgccgta gtggtaccag
     1501 tcgccgcaga gccgtgcggc gtaggtgtac tcggtttcgc tgagcgtgtc gcgcgcggcg
     1561 caggtgaagc gcttgctccg gtacacctga tggcagtcga aggccgcgcc gcatttgtgc
     1621 tcgtccgggt agagtacgca gccgacggtg cgctcgccgt cgatgtaccc cacgaagggg
     1681 cactggaagg cgttttcgac gcgcttctcg atgacgtcgc tcatgacgcg ctccccgcgg
     1741 aaatagtacg agccggtcat gtcgctccgg ctcccgcgga cggcgccgat cagatagccg
     1801 cggctgaact gcgaaaggag cgcggcgcgc cgttcgaaga gcgcgtcgat ctcggcgtag
     1861 ggcgcgcgat agttgtggct gcagcaacag agggcgcacg aggcgccgtg gcccggccga
     1921 cagatggagc gttcggttgt catgaatgcc ctagtcttcg tcgaacgacg ggggcgcctc
     1981 gcgggccagg atgtcctgga gctgggcgat gagctccccc tggtcgatgg gccgcgggag
     2041 gtagcgcacc tcgaggtttt cgcagtggct cacgaactcc ggggggtcgg cctcgcccac
     2101 gaagaggatc ggcagcggcg agaactcctc gctgttcttg atgacgcgga tggcgttgag
     2161 gttggcgtgg tagcggtcct cgccggtctt gccgatcacg atgaaggccg aaacgtcgcc
     2221 cagccgctca tggtgctcgc tgatgtacac cgagaagacc gagaacgagc tcgccttgat
     2281 gagggtgacg cccagcacct gcttgatgaa atcgaactcg tccagcgctt ccctggcgcc
     2341 gccgatgatg acgatgattt tcttcatggt gggcagattc tatcgcagcc ggaataaaat
     2401 tcaacgaata aatggggaat attcaatttg acaatgcgga taatgcggcg gccttatggt
     2461 ggtggacgca ttgcaatcgg gtcgttcggg cgcagtcgac cgttccgtcg cggggaggag
     2521 gcgtgaagtc catcgatagt attgtgctat gcctgctcgt ttcactcttt tggctctgcc
     2581 atgcgcaggc ggcgccgccc gcgtcctttc acggcgtttt ccatccctgc tacgacgaat
     2641 cgaagaagaa atatacggta tgcgagcggg ggaggcgcgt ccgcatcgat cgtatcatgc
     2701 catgcgctga aggggtgtat accaacccgg accactggtt cgacgaggtg cgcccggcca
     2761 tgaaggggcg ggacgccaac ggagccgagt atcgcatcgt gtatgtcgga tggaaataca
     2821 acggggaagt cgcttcgtgc gggtttgaaa tattcagggc cgggacacat cattccggct
     2881 ggcacatgca cttctggttc gtgaagaacg ataatgaatg gcggctgctg gagatcgtca
     2941 aagtgccgag cggcaggatc gactttcgca tcaccccgtc cttcgggccg tcgggtctgt
     3001 ttccgttcca gtactattac ccggtcaccg ccacaacgcc gccgagcggg ttcggttttg
     3061 tcgatgtaaa cggtgttgtg gtcatcaagc ccgaattcaa tatcgcgcat ccgttttcgg
     3121 acggcctcgc gctggtcgcc cgccgcccgc cctcgaagcg cggccgatgg gacaggtata
     3181 gccgcgaatg gatgtatatc gacgtccgtg gggcgaccgt tatcgccccg gcgcggtacg
     3241 acaagccgcc tcgttcattt cgaagcgggc gcgccctcat tgagaaaaat gggtatgccg
     3301 gtttcatcga tacgaccggg aagattgtca tcccgccaga tcggttcgat cgtactaaca
     3361 cgaccgattt ttccgaaaag gggattgcgc gagtatcgtg gtggcatgac cccgccgcga
     3421 attattgtta ccggcgcatc aatgcctgtc atagctgtct cacctttcgg tacctggccg
     3481 gaagaatcgg cggatcgccg ttcatatacc tgaaggaaaa aatcaggatg tacggcgtaa
     3541 tcgaccgaaa cggcgcggtg ctgtattcgt cggagagctc cgacgaggtg gggcgctggg
     3601 tgtcgaagcg gtactatggt gacgagagtt gatgctgtgc aagatgtcat cgcgagcgaa
     3661 acgaagcgat cagtattgca aatatcgggc tagatacata cgagcctgag agttcacatt
     3721 tttgcgaaga aaatgccgga ttgcattatc gctatcgctc ctcgcaatgg gcattgcgaa
     3781 gaaacgcctc gatcgcatcg atcagctcat gcgctttgtg aatcatttcg tcggcctttg
     3841 cggcgttaac ggagttgatg attccatagt cgccaatgtt gcgaatctca aaggcctcca
     3901 ggacgtgacg atggtatcgg tcgtcgaaca agccggtttt aacgaagtcc ttgccgaagg
     3961 ctgcaatgac ggcgctgtgc tttgagtatg attggtttcg gctgaggagc aacgcctcaa
     4021 gcgcatagaa catcgcgtag tatgaccgcg cggctgagaa atcggtatag cgatcatgaa
     4081 ggagcagtgt ggccgcatct aggctctggc gggccttttg cagaagctgc tgaatctcgc
     4141 cggtcattgg atggcaatgc cctctctgat gatgtttaat atcaggggcg tgcgagcagc
     4201 tttaaagtaa tcgctattga taacggtaat tgatacgacc gtgtcgtgtt tgagggcaat
     4261 cgggctgatg tgcggcagat accgctcgag ttcgtccatc ggcgtaacgg cgccgtcgag
     4321 caggattgcc acatcgatgt ccgagccatc catgtagtcc ccgcgcgcat acgagccgta
     4381 taaaaacatt cctttcaggc gggatgtgta caattctaac aatgttttcc gtgcttcgtt
     4441 caataccggc acgatctgtt ctgggattct cggtttcatg atttcagtta aatcggaaga
     4501 gcctgtttcg ctcagtatgc gtctggcgga atggttcgca accatttttt ctcacgattg
     4561 ctttcgtgtt ggggtggtgg catgccggtt cattatgtcg ggttcgtacg gctaaaacgg
     4621 cagcttctcc cgcaaaagcg cgatgaccga gttcggctgg acgttcgcct gcgcgagcat
     4681 cgccatgttg gtctcggcga tgatggtgtc cttggtgagc gcgaccatca cctcggccac
     4741 gtcggcgtcg cggaccacgc tggaggcgcc gtgcgcgctc tcccatgctt gcgagagctc
     4801 ctgcgcggtc ttctcgaagc ggctgatgta ggcgcccagg tccgagcgct gcttcacgat
     4861 gcgctggatc gcatggtcga gcagaccgat cgcgtcgttg gccgcctcgg gcgagctgag
     4921 gctcgcggcc agcgccccgc tatcgttccg gatgcccagc ccccgcgcgg tcatggtgcc
     4981 gatgtagatg cgctccttct ggaagctgtt gggccccacg tggaaccaca tgctcgcgtg
     5041 ggggttgtgc cgcgagaaat cgcccagcat gatcttcatc gtgttgaagt cggcctgcga
     5101 ggccacgcgg tcgatctcgt cgacgagcgc cgagatctcc acctggatga gctgccggtc
     5161 ggccggggtg taggtgccgt tcgctgcctg gacggcgagc gaccgcatcc gactcatgat
     5221 gtcggtcgat tggcggaggt actcctcggc ggtgttcacg attgagatgc cgtcctcggt
     5281 attgcgctcg gcctgccgca ggccgtggat ctgccccgtc atcttttcgg acaccgcgag
     5341 ccctgcggcg tcgtcgcccg cgcggttgat gcgcagcccc gacgagacgc gctcggccga
     5401 cttgccgatg tcccagttct tgaacttgag aatattgctc aagttgatgg cgtgcaggtt
     5461 gcgattgatg atcatgccga tggtaccccc actcatagtt tcggcgccgg tacggtaata
     5521 cttcagcaat ttttttgtgg cttgacggat tcgcgatcat gtgcacgatt attgtcgtgc
     5581 gcagggcgca cgtttttcat atatggatcg tgcccgccgc gcgtatgccg tgcgtgcata
     5641 ggaaataata gtggaaggaa cggaaaaaaa gaaaatactc atcgtggagg attccgcgac
     5701 cgcggcgctc gcgacgtcgc atgtcctggg tcaattcggc taccaatcga tcatcgtcga
     5761 caccggcgag gctgcggtcg aggccgttcg gggcgatcca tcgctgtcgc tggttctcat
     5821 ggacattgaa ctggggtcgg gaatcgacgg cgccgaggcc gcgcgccgca tcctcgccgt
     5881 ccgggccctt cccatcgtgt ttctcaccgc ctacgccgag gagcacatgg ccgagcgcgt
     5941 ccgcggcatc acccgttatg ggtacgtgat caaagactcc agcgagtatg tgctgcaatc
     6001 ggccatcgag atggcctacg agctcttcga tgcgcattgc cggatcgcgg agagcgaaga
     6061 gcgatatcgc ctcctttcgg agaacaccga tgacgtgatc tggctctggg acctggcggg
     6121 gaaccggtac gagtacgtcg gctcctcggt gctgcgcctg ctcggcttcg ccgtcgacga
     6181 ggtgatgcgt caatcgctgt ccgacgcact gccggcgaag catgccggat tgctcaccga
     6241 ggagctcccc cggcgagtcg ccgcgcttga ggcgggggac gagtcggccc gtttcaagac
     6301 ctacgcggtc gatcaatacc ataaagacgg taggctgatc ccgacggaag tgtcgacctc
     6361 tttggttacg gatcttcgcg gacgggtgac gggactgcag ggcgttaccc gcgacatttc
     6421 cgagcagcgg cgcctcacga ccgagctcgc gcaggcgaac cggcacctcg ccgcgctgtg
     6481 gggcgtcgct gagctggccg atgcgggctt caaggatatc tgcgaccaca tcctgcgagc
     6541 cgtcgtggag atgaccggga gcggctacgg cttctacgga ttcatcaacg acgacgaatc
     6601 ggtgatgacc atccatgcct ggtcgggcga cgcgatgcgc gattgcgccc tggtcgacaa
     6661 gccgcaggat ttttccatcg cgagcgcggg agtgtgggcg gaggcggtgc gtcgccggga
     6721 ggcgctggtc ataaacgatt ttgcggcgcc gcacgacgcc aagcggggtt atccggcggg
     6781 tcatgtcgcg ctcacgcggc tcctcgcggt gccggttttc tcgcgcggca ggatcgtgtc
     6841 cgttgcggcg gtcgcgaacc gggagacacc ctacgggcct gcggacgtga gccaggtggg
     6901 cgcctttctc acgggcatca tgtcggtcgt cgagcggcgc cgcgccgagg aagaggtgcg
     6961 ccgcctcctc gcggaaaagg agctcctgct gcgcgaggtg caccatcgca tcaagaacaa
     7021 catgtccacc gtcatcagcc tgctcgatat tcagtcgaac gcgccgggga acgaggccgc
     7081 cgggcccgcc ctcgaggagg ccaaccggcg cctgcgggtg atgatgatca tttacgacaa
     7141 gctctatcgc tcgaaggaat acggcgcgct ctcggccgac cgctacctgg aggagttcgt
     7201 ccccgacatg ttctcgggtt tcccgcacgg cgggagggtg cggctcgaca tggccgtcga
     7261 gccgatttcg ctggacgtga agttcctgtt tccgttgggg attatcatca acgagctcct
     7321 tgcgaactcc atgaaatacg ccttcccgga ggggcggacg ggcaccgtcc ggatcggcct
     7381 gcgccgctcc gacgcgcatg ccgtcgagct ggtgtttcgc gacgacggca taggtttcgc
     7441 gggggacgtg gacctcgcct cggcgcgcag ctttggcctg cagcttgtgt cgctcttggt
     7501 gcgccagatc aacggcgctg tcgccgtgaa cgggcgcgac ggggcggagt acgtgatcac
     7561 cttcgccgca tgatgcgcgc tcgatagacc atcacacgag aggaggcggg aatggcgaac
     7621 gatccattgg cggtgatacg ggaatccgac ccggcgttgt tcgcgacgtt gggcgacatg
     7681 cgcgagtgcg cgctgggcga gggcgcgcta tcgaagaagc acaagctcct gatcgcgctc
     7741 gccatcgacg ccgcgatcaa gtccgagaac ggcgtccgct ccctcgcgcg gcaggcgctc
     7801 gaggccggcg cgtcgaggcg ggagatcatg gaggcgctgc gcgtcgtgaa ctatatctgc
     7861 ggtgcgggaa gcatgtacgc ggcggccgcc gcgcttgcgg acgtgtttcc cgatgcgtga
     7921 tcgcgcgata gcggaggtgc catgatgtcg accgctttca ggatcatccc ggttgtcgcg
     7981 gtgctgcttg cgtgcctgtg gggcagcgca ccgccggcgg cggcgcaatc gaagaagatg
     8041 accatcctcg agtactacaa ttctttcgtg atgtggaaga agctcgggtt cgaacacctc
     8101 atcgaccgga agatcgggcc ggaggggcgg caggagcatg cgagccgggg cgcgtatatg
     8161 tacaatatca tcgtcgacgc cccgaacggt tttctgcaat acgcgaccgg ctgctcgtgc
     8221 caccaggaga taaattccta tgcgatcttc aagaccgccg gcggcgatgt gtatctttcc
     8281 gcgagcggcc ggaacctgaa cttctataaa ttcgtcggtg caaacaaatg gatcgacgaa
     8341 acgaaaacgg tcgtgccgga gaccggcctg ggggcctttc tcgccgaggg cgcatcgccc
     8401 gcatcgattc cggctaacgt gaggaaatgc tccgcgatct tctatgcggt gccgcgctat
     8461 gggaccgtcg tgactgcgga gctgcggctg tattcgaaac accaggacga ccccgaacgc
     8521 tccgagcagg gatggaagcg cctcgaggcc ctccgcccgg ccttccggaa ggcctatcgg
     8581 tacacgaagc tcaatctgcc ctgggaccgc gagcggggca tcatcaccat cggaaaaaag
     8641 gaataacgtt ggacgcgcat gggggatgat tccgacgtgc gggcgaaaga gcggcccgcg
     8701 gttctcgcaa cgcacaccgt cccggagggg accgatggcg cgcgtttgag cgagtacgcc
     8761 atcggcgtct tcgagatggt gccgacccga aacgggatca agaacgccat caggcgcggc
     8821 acggtgtcgg ttgatggtgc gcccggtaca acggcaacgc gggtgagggc cggacagcgc
     8881 atcgacctca tcgacgctcc gggccccgtc cgtcccgtgt acgaaatgaa actccacgtc
     8941 gtctacgagg acgagcatct cgccgtcgtc gacaagcccg cagggatcct ggtgagcggg
     9001 aacgccttcc gtaccgtcga gaatgcgctg ccgttcaatc tcgccgcatc gggcattcga
     9061 tccgcgcttg cgcggccgcg cccggtgcat cggatcgacg cccagacgag cggcctgctg
     9121 ctggtcgcga agacgtcgct ggcgctcgtc gcgctcgggc gcggcttcga ggggcgggac
     9181 atctccaagc ggtatcgagc gatagttatc ggtgacatcg ccggccccgg ccggatcgac
     9241 aaccacatcg acggccgcga tgccgtcagc gaatacgctc cggtcttttc cgttccctcg
     9301 ctccgaagcg gtcgtctcag cctcgtcgat ctctttccgc gcaccggccg cacccaccag
     9361 cttcgccgcc acctggcgca tattggccat cccattctgg gcgattcgct ttatggcccg
     9421 gaagggctca tcctgagggg gaaggggctc ttcctggcgg ccgtggagat ctcgttcgcg
     9481 catcccgtcg gtggcgcccc catgcgattc gccatcgacg agcccgccaa gttccgcacg
     9541 ctgctcgagc gcgaacgacg gcgctgggaa aaattccacg aatagggcct ttccggttcg
     9601 caatttctgt tcgtgcggga attgatgaga cgggcgtgag gcttttttgt atttttctat
     9661 ttattagact ttataacctc atcccctgat atgatgtgac cccaatactt tgcactaccc
     9721 accagacatc gggtat
//
LOCUS       DAORHF010000135         9664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6427_length_9664_cov_4.912062, whole genome shotgun sequence.
ACCESSION   DAORHF010000135 DAORHF010000000
VERSION     DAORHF010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6427_length_9664_cov_4.912062"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PKJ16_11625"
     CDS             <1..505
                     /locus_tag="PKJ16_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HNV47682.1"
                     /translation="PFRKSIQLDLLRCYFYYCPPWMSPEPTDSEKKRMEEHVTFMEHI
                     ESLDRWSVRLGKLQRRWEGGRELYEQKRVDVLLSVDMVRHSAAGHIQHAIIIAGDSDF
                     IPAIEATKESGATVTLWYGDPNTIHKDLIQVSDIVHQMDFKKVPSEKIPESEQGSSAR
                     RFWRRRR"
     gene            510..2021
                     /locus_tag="PKJ16_11630"
     CDS             510..2021
                     /locus_tag="PKJ16_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV47683.1"
                     /translation="MRYASRRPVRAPRIVRVRRRIGALAAILLAFGASCLSLPYSEHS
                     PTVSADGRILIYQSNYLTIHKYKLFVKYRTRVGWTPAFMMVDVGSSANDGGPFITYDQ
                     NNLLLSSTRRGGIGSIDIWTARRLGPIWTIPDNIGAPINSPAYDGFASLSPDGSTMYL
                     VRECPDKRLARDERFGIFVARKIDDAWSAPVKMPAPINSEHSEFSPLILADGATLIFS
                     SDRPGGRGGYDLYQSRYVNGRWSAPVSMDFINTPEDDRLVSVPASGDIIYYSRPIVVE
                     GQTVYRITSAELPARFRPAPVALVHGTVFDLDAKEACPGAAITITDVRGTVAPVHIHA
                     NQKDGRFVAILNRGRLYDVSVAAKGYTFRSERIDLRALTKYASVEKDITLRRLRPGAT
                     MVLNTLYFAVNSARLLPESVFELRRVIELMNAYPSMSVSIAGHTDSTGVEEANVRLSR
                     DRALSVARHLAANGIAAGRITTKGFGSSKPVSPNDTDENRRANRRVEFTVETM"
     gene            2037..3065
                     /locus_tag="PKJ16_11635"
     CDS             2037..3065
                     /locus_tag="PKJ16_11635"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47684.1"
                     /translation="MNHRTARRVASTAYRVMILAASCVALSISAFAQENAARYTGDPR
                     TIAHDFLDLEKDFGARPEHHATVNRIINRALAEVPVKPDAATADAVRTLQAIDAVLRA
                     EGFSFKDNLLLSVGIARKQIDCDNYCALYVAIAEVMKIPLVPVYAPDHSFVRYYFSDG
                     SFLNWEPTQGKSLPDSFYVKAKGIAQPSIEKGVYLKALSRKEFVAVEYNNIGAHLIMA
                     KKLREAVTYLSAAIAFYPAFSSAWHNRGSARLALAMLPEARSDLETAVGLDPLRATSR
                     TALGDAYSDLREYQKAVQHYAASIKLDPTNHIPYHNMAVAMQRLGRDGDARAWEKKAK
                     EIRAKYGR"
     gene            complement(3072..4733)
                     /locus_tag="PKJ16_11640"
     CDS             complement(3072..4733)
                     /locus_tag="PKJ16_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV47685.1"
                     /translation="MSEKRKIVIIGGVAGGASAATRARRLSETASIVIVEKGPYVSFA
                     NCGLPYHLGGVIQERDALLVQTPQSLKERFNIDVLVNAEAIAIDRNARTVLVRNLITG
                     ADDTLTYDALILAPGAEAVRPPLPGLDLPNVFTLQTMSDMDGIIAHVRGTRVKRAAVI
                     GGGFIGVEAAENLAGRGIAVSLIEADRQVLPPLDPEMASPLHAHLRENGIDLRLGAKA
                     SACAADGDGIAITLDGGGIVSADLVILAIGVRPRTDLAKRAGLEIGETGAIKVDDAMR
                     TSDSAIYAVGDAVEVTNPVTGLPAFIPLAGPANRQGRIAADQIFGRDSRYRGTIGTSI
                     CKVFSLAAGATGASEKLLRRIGRPYQKTYLHPGHHAGYYPDAESISLKVIFDTASGAI
                     LGAQAVGAKGVDKRIDVIAASIAAGRTVFDLEHLELAYAPPYGSAKDPVNMVGFIAGN
                     ILRGEHRIIHAEEIGALDPARDMLLDVRTVEENAEGRMPNSINIPINELRGRIGELPR
                     DRRIVVYCAVGQRGYVAQRMLTQMGFDAVNLSGGYATWIMAQAAR"
     gene            4913..5860
                     /locus_tag="PKJ16_11645"
     CDS             4913..5860
                     /locus_tag="PKJ16_11645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV47686.1"
                     /translation="MAKILIIDDMPGNIKTLGEILRDRNDIIVATDGPGGIALAASEA
                     PDLILLDIMMPDMDGFAVCRALKERTKTASIPVIFTTALTDTDDIVRGFESGAVDYIT
                     KPFNPRELIARVNTHLELVKARDQLTIYAESLEEMSRQLLDKTTQLNISARTDYLTGL
                     ANRIHMMERLREESARSRRGGKPCAVIIADIDHFKAINDTHGHECGDHVLGSIADIMR
                     ANIREQDVLARWGGEEFLFLLPETDAAGSSVLAEKIRGAVECERISYHDTIVPVTLTL
                     GVASLNPEDDIDASIRRADEALYEGKRTGRNRVVVAPGA"
     gene            complement(5928..7091)
                     /locus_tag="PKJ16_11650"
     CDS             complement(5928..7091)
                     /locus_tag="PKJ16_11650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47687.1"
                     /translation="MSTHIAPTILIVDDMAVNLQVIGAFLKEQDYRISVAKSGAEALA
                     IVAAASPDLILLDIEMPEMNGYEVCRRLKDDPSTREIPVIFLTSHDTADDIVSGFDAG
                     AVDYVTKPFNRSELLARVRTHLELGRSREEIAHAYRQLMLKDRIMTDDLAKARDIQGH
                     VFLIDHDAFPEIEFGVHYQPFIQVGGDMYDACRVRNGHCRFFIADAIGHGIQAALVTM
                     LIKSVYDKFKEDDIAPGQLLARLNDSFYHTYYRLLVLFTACIVDVDVAGGRLFVANAG
                     HPSPYLVGSGRLRQLPTTGGPLIGVQIDRTYGTVEVPFAADECVIMYTDGLYEDLLQA
                     RSHIEMDDVEEVMLRHADERPEDVARNIVRDIGSALDGAPFNDDVTVLAVRRR"
     gene            complement(7153..>9664)
                     /locus_tag="PKJ16_11655"
     CDS             complement(7153..>9664)
                     /locus_tag="PKJ16_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47688.1"
                     /translation="FGDLVQRFRRVSSIQSELSLPLGDGLRHFDVRISPLHDRSGAVI
                     ARVVLVRDITDRRQAEDEMRLNEARLEALYRLNQGEGATEQELVDRALAEALPLVASD
                     IGRMVVRGADVGDAEHAASCGNPDAIVLDDMDALRQPGKADWWAESAWALAPIIHERG
                     FAMTGGDAAPAPIRVMSVAHVKNGEAVIVLGCAGKPDPYSQLDVHQLTLYLEGVWKII
                     EKKRVDMALARALENAQAAAKAKSEFLANMSHEIRTPMNPIVGLTHLIQQTELTERQR
                     DYIGKIQDSAQLLLGIINNILDFSKIEAGRLDLEIVDFDLATLLGALSNLFARTVEDK
                     GVEFRMQVDPDVPVCLRGDPLRIQQVLGNLIGNAIKFTERGYVGVRISRLPGPGGPDR
                     AVVRFSVEDTGIGIELDQRERIFKPFTQADASITRRFGGTGLGLAIVRNLVELMGGSI
                     VVESRPGEGSRFSLDVPLGVCAGGVWGDGAIGPIDVTRARIEHRAAVKTDATADRRIL
                     LVEDNLINQLVAMEVLKGAGMTVVPTGNGREAIDMLRKSRFDLVLMDLQMPEMDGFEA
                     TRIIREDPMLAHIPIVAMTAHAMQRDRDRCLSAGMNDHIAKPIDPPELVAMITKWLPG
                     SVADRGGISPPELSSGTDLPETPHGIDLADGLYRLGGNAALLRSVILDFCAEFEHAAD
                     DLARFMEVGDLEAARKLVHAIKGAGGNIGAVALHAAAKDLEASLKAGDAESAAGARAS
                     FSSALAEVLKNRELFGRDTSKNDARAVDMTAARSLLETLHEQVSRNSFECVDTFRRLK
                     NAMGTCREDEVERLGAHINGFDFPNARIAIEKLLNDIS"
BASE COUNT         1810 a         3232 c         2900 g         1722 t
ORIGIN      
        1 accctttcgt aagtcaatcc agctcgacct tcttcgctgc tatttctact actgcccccc
       61 gtggatgtcg ccggaaccca cggacagcga gaaaaaacgc atggaggagc acgtcacgtt
      121 catggagcat atcgaatcgc tcgaccgctg gagcgtccgg ctgggcaagc tgcagcgtcg
      181 ctgggagggc ggtcgcgagc tctacgagca aaagcgggtg gacgttctcc tctcggtgga
      241 catggtccgt cattccgccg ccggccacat tcagcacgcc atcatcatcg cgggcgacag
      301 cgactttatc ccggccatcg aggccaccaa ggaatcgggc gcgaccgtga ccctctggta
      361 cggcgatccg aacaccatcc acaaggacct catccaggta tccgacatcg tgcatcagat
      421 ggacttcaag aaggtcccgt ccgagaaaat ccccgagtcg gaacagggct cgagcgcccg
      481 tcggttctgg cggcggaggc gataacgcag tgcgatacgc gtcgcgtcgc cccgtccgcg
      541 cgccgcgtat cgtgcgggtg cgccgccgga tcggcgcgct cgcggcgatt ctgctcgcat
      601 tcggcgcctc atgcctctcg cttccctatt cggagcacag tcccacggtc tcggccgacg
      661 gacggatcct tatctatcaa tcgaattacc tcaccatcca caagtacaag ctcttcgtga
      721 agtaccgcac ccgggtgggc tggaccccgg cgttcatgat ggtggacgtg ggctccagcg
      781 cgaacgacgg cgggcccttc atcacctatg atcagaacaa ccttctgctc tcgtcaacgc
      841 ggcgcggcgg gatcgggagc atagacattt ggacggcgcg ccggctgggg cccatctgga
      901 ccattccgga caatatcgga gcgcccatca attcgcccgc ctacgacggc ttcgcgtccc
      961 tgtctcccga cggatcgacg atgtacctgg tccgcgagtg cccggacaag cggctcgcgc
     1021 gagacgagcg gttcggcatc tttgtcgcgc gcaagatcga cgatgcatgg tctgcgccgg
     1081 tgaagatgcc cgcacccatc aactccgagc acagcgaatt cagcccgctc atcctggccg
     1141 atggcgcgac cctcatcttc agctccgacc ggcccggcgg gcgcggcggc tatgacctgt
     1201 accagtcgcg ctacgtgaac gggcgctgga gcgcgccggt gagcatggac ttcatcaaca
     1261 ccccggagga cgaccggctc gtctcggtgc ccgcctcagg cgatatcatc tattactcgc
     1321 gtcccatcgt tgtcgagggc cagaccgtgt atcgcatcac ctccgcggag cttccggcgc
     1381 ggttccgccc ggcgccggtc gcgctggtgc acggaacggt cttcgacctc gacgcgaagg
     1441 aagcctgtcc cggcgccgcc atcaccatca ccgacgtgag gggaacggtt gcgcccgtgc
     1501 acatccatgc gaaccaaaag gacggccgct tcgtcgccat cctcaaccga gggcgcctgt
     1561 acgatgtctc ggttgccgcg aagggctaca ccttccggtc ggagcggatc gacctgcgtg
     1621 cgctcacgaa atacgcgagc gtcgagaagg acatcaccct acggcgactg cggccgggcg
     1681 cgacgatggt gctcaacacc ctgtatttcg ccgtgaacag cgcgcgactg ctcccggagt
     1741 cggtcttcga gctccgtcgc gtgatcgagc tcatgaacgc ctacccgtcg atgagcgtgt
     1801 ccatcgccgg ccatacggac tcgacgggcg tcgaggaggc caatgtgcgg ctctcgcgcg
     1861 accgggcgct ttcggttgcg cgccatctgg ccgcgaacgg catcgccgcc gggaggatca
     1921 ccaccaaagg gttcggctca tcgaagccgg tgtcgccgaa cgataccgac gagaaccgcc
     1981 gcgcgaaccg ccgcgtcgag tttaccgtcg aaacgatgta acccgggagg gaccgcatga
     2041 accatcgaac cgccaggcgt gtcgcatcca cggcgtaccg cgtgatgatc ctcgccgcga
     2101 gctgcgtcgc gttgtcgatt tccgctttcg cacaggagaa cgccgcgcgc tacaccggcg
     2161 acccgcgcac catcgcgcac gacttcctcg accttgaaaa ggacttcggg gcgcgccctg
     2221 agcaccacgc gacggtgaac cgcatcatca accgcgccct cgcggaagtg cccgtgaagc
     2281 ccgatgccgc caccgcggac gcggttcgga cgctccaggc gattgacgca gtcctgcgcg
     2341 cggagggctt ttcgttcaag gacaaccttc tgctctccgt gggcatcgcg cgaaaacaga
     2401 tcgactgcga caactactgt gcgctctacg tcgccatcgc agaggtgatg aagatcccgc
     2461 tggtgccggt gtacgctccc gaccacagct tcgtccgcta ctacttctcc gacggatcat
     2521 ttctcaactg ggaacccacg caggggaaaa gcctgcccga ctcgttttac gtaaaggcca
     2581 agggcatcgc ccagccgtcc atcgagaagg gggtatatct caaggcgctc tcgcgcaagg
     2641 agtttgtcgc ggtggagtac aacaatatcg gcgcgcacct catcatggcc aagaagctcc
     2701 gggaggccgt gacctacctg agcgccgcga tcgcgtttta tccggcattt tcgtcggcct
     2761 ggcacaatcg gggctccgcg cggctggccc tcgcgatgct tcccgaggcg cgatcggatc
     2821 tcgagacggc cgttgggctc gatccgctgc gcgccacctc ccgcacggcg ctgggcgatg
     2881 cgtattctga cttacgagag taccagaagg ccgtccagca ctatgccgcg agcatcaagc
     2941 tcgatcccac caatcacatc ccctatcaca acatggcggt ggcgatgcag cgtctcggcc
     3001 gcgacggcga cgcgcgcgcc tgggagaaga aagcgaagga gatacgggcg aaatacggcc
     3061 gctgagggac gctagcgcgc ggcctgcgcc atgatccagg tggcgtatcc gccgctcagg
     3121 ttcacggcat cgaagcccat ctgcgtgagc attcgctgcg cgacgtatcc ccgctggccc
     3181 accgcgcaat acaccacgat gcgccggtcg cgcgggagct ctccgatccg tccgcgcagc
     3241 tcgtttatgg ggatgttgat ggagttcggc atgcgcccct cggcgttctc ctcgaccgta
     3301 cgcacatcga gcaacatgtc gcgtgcggga tcgagtgcgc caatctcctc agcgtgaatg
     3361 atgcgatgtt ccccccgcag gatattcccc gcgataaaac cgaccatgtt caccgggtcc
     3421 tttgcggaac cgtagggcgg cgcatacgcg agctcgagat gttcgaggtc gaacaccgtg
     3481 cgcccggcgg cgattgacgc ggcgatcacg tcgatgcgct tgtccacccc cttcgcgccc
     3541 acggcctgcg ctcccagaat cgcgccgctc gccgtatcga agatgacctt caatgaaatg
     3601 gactccgcgt cgggataata ccccgcatgg tgccccggat ggagataggt cttttgatac
     3661 ggcctgccga tccgccgcag cagcttctcc gacgcgccgg tcgctccggc ggcgagcgaa
     3721 aataccttgc agatcgatgt cccaatcgtc ccgcgataac gggagtcgcg cccgaagatc
     3781 tggtcggcgg caatgcgccc ctgacggttg gcgggccccg cgagcggaat gaaggcggga
     3841 agccccgtca ccgggttcgt cacctcgacg gcgtcgccca ccgcgtagat cgcggaatcg
     3901 gaggtgcgca tggcgtcatc gaccttgatg gcgccggtct cgccgatttc cagcccggcg
     3961 cgtttcgcga ggtccgtccg cggccgcacg ccgatggcaa ggatcaccag gtccgccgac
     4021 acgatgccgc cgccatccag ggtgatcgcg atcccgtccc cgtccgccgc gcatgctgac
     4081 gccttcgcac ccaggcgcag gtcgatcccg ttctcgcgca ggtgcgcgtg aagcggcgat
     4141 gccatctccg gatcgagcgg cggcagcacc tgccggtccg cctcgatgag gctcaccgcg
     4201 atgccgcgtc cggcgaggtt ctccgccgcc tcaacgccga tgaagccacc gccgataacc
     4261 gcagcgcgct ttacgcgggt accgcgaaca tgcgcgatga tgccgtccat gtccgacatc
     4321 gtctgaaggg tgaacacgtt cggcaggtcc aggccgggca gcggcggccg cacggcctcg
     4381 gcgccgggag cgaggatgag cgcgtcgtat gtcagcgtgt cgtcagctcc ggttatgaga
     4441 ttgcgaacaa gcaccgtgcg cgcgttgcgg tctattgcaa tggcctccgc gttcaccagc
     4501 acgtcaatgt tgaaccgctc cttgagcgac tgcggtgttt gcaccaggag cgcgtcgcgc
     4561 tcctggatca ccccgcccag atgatagggc aatccgcaat tcgcgaagct tacatacgga
     4621 cccttctcga cgatgacgat gctggccgtt tcgctaagcc gccgcgcccg cgtcgccgcc
     4681 gacgccccgc ccgcgacacc gccgatgatg acgattttcc ttttctctga cacttggcat
     4741 gaaccttccc atgattagat actatcgttc tcatccaacg aaaaagtaat agtatgcatc
     4801 aaaatcattc cattttcaag aaatttctcc acacccgaca tatcgcattt cactgttgac
     4861 aaaaaccctc gcccgcatca tgctatcggg agaatcggca ccggtacgca ctatggcaaa
     4921 aatcctcatc atcgacgaca tgccgggaaa catcaagacc ctgggcgaga tcctgcgcga
     4981 ccggaacgac atcatcgtcg ccacggacgg ccccgggggg atcgcgcttg ccgcgtccga
     5041 ggcccccgac ctcattttgc tcgatatcat gatgcccgac atggacggct tcgcagtgtg
     5101 ccgggcattg aaggagcgga cgaagaccgc gtcgatccca gtcatcttca ccaccgcgct
     5161 caccgacacc gacgacatcg tccgcggctt cgagagcggc gcggtggact acataaccaa
     5221 accgttcaat ccccgggaac tcattgcgcg ggtgaacacc cacctggagc tcgtgaaggc
     5281 ccgcgaccag ttgaccatct acgccgagtc gctcgaggag atgagccggc agctcctcga
     5341 caagacaacg caattgaaca tttcggcgcg aacggactac ctgaccgggc tggccaatcg
     5401 gatccatatg atggagcggc ttcgcgagga aagcgcgcgg tcccggcggg gcggcaaacc
     5461 ctgcgccgtc atcatcgcgg acatcgatca cttcaaggcc atcaacgaca cccacggcca
     5521 tgaatgcggc gaccacgtcc tggggtccat tgcggacatc atgcgggcga atatccgcga
     5581 gcaggacgtg ctcgcgcgct ggggcggcga ggaattcctt tttctgctgc ccgaaaccga
     5641 cgcggcggga tcatcggtcc ttgcggagaa gatccgaggg gcggtcgagt gcgagcggat
     5701 cagctatcac gatacgatcg tgcccgtcac cctcaccctc ggggtcgcct cactcaatcc
     5761 agaggacgac atcgacgcca gcatccggcg ggccgatgag gcgctctatg agggcaagcg
     5821 cactggcagg aaccgcgtgg tcgtcgcgcc cggggcgtaa tcgccgaaat cattccgcgc
     5881 gtatcgcggc gtcgggcgca catccacgct ggtatccaga cctcgcatca gcgccggcgc
     5941 accgccagga ccgtgacatc gtcattgaac ggggcgccat cgagggcgga tccgatatcc
     6001 cgaacgatgt tgcgagcgac atcctcaggc cgctcatccg cgtgacgcag catcacctcc
     6061 tcgacatcgt ccatctcgat gtggcttcgc gcctgcagga ggtcctcgta caggccatcg
     6121 gtgtacatga tcacgcattc atccgcggca aaggggacct ccaccgtccc gtatgtcctg
     6181 tcgatctgga cgccaatcag gggccccccg gtcgtaggca gctggcgaag ccgaccggat
     6241 cccacgagat agggagatgg atggcccgcg tttgcgacga agaggcggcc gcccgcgaca
     6301 tccacatcga caatacatgc cgtaaagagc accaacagac ggtaataggt atgatagaaa
     6361 ctgtcgttga ggcgcgccag gagctgcccg ggggcgatgt cgtcttcctt aaatttgtcg
     6421 tacacgctct tgatgagcat ggtcaccagc gccgcctgga tgccatgccc gatggcgtcg
     6481 gcgatgaaaa accggcaatg cccattccgc acccggcagg catcgtacat atcgcccccc
     6541 acctgaatga agggctgata atggacgccg aactcgatct ccggaaacgc atcgtggtcg
     6601 atgagaaaga cgtggccctg tatatcgcgc gccttcgcga ggtcgtcggt catgatccgg
     6661 tccttcagca tcagctggcg ataggcatgg gcgatctcct cgcgcgagcg cccgagctcc
     6721 aggtgcgtcc gcacgcgcgc gaggagctcc gatcggttga acggcttggt cacgtaatcg
     6781 accgcgccgg cgtcgaaacc ggagacaata tcgtcggcgg tatcgtgcga ggtgagaaag
     6841 atgacgggga tctcgcgcgt cgacgggtcg tccttcaggc ggcgacacac ctcatacccg
     6901 ttcatctcgg gcatttcgat gtccaggagg atcaggtcgg ggctcgctgc ggcgacgatg
     6961 gccagcgcct ctgcgccgct cttcgcgacg gaaatccgat agtcctgctc cttgagaaag
     7021 gcgccaatca cctgcagatt taccgccatg tcgtcgacga tcaggattgt cggggcgata
     7081 tgcgtggaca cgtgcggcct cccattttcg tttttcacct gccggacgac gcattccctg
     7141 aggatgctcg tttcatgaga tatcgttcaa aagtttttcg attgcgatac gcgcattcgg
     7201 aaaatcgaat ccgttgatat gcgcgcccag acgctcgacc tcatcctccc ggcaggtccc
     7261 cattgcgttc ttcagccgcc ggaaggtatc cacgcactcg aagctgttgc gcgaaacctg
     7321 ctcatggagc gtctcgagaa gcgagcgtgc cgccgtcata tcgactgccc gtgcgtcatt
     7381 cttcgacgta tcccggccga agagctcgcg gtttttcagg acctcggcca gggccgatga
     7441 aaaggacgcg cgggccccgg cggcagattc ggcatcacct gctttcaggc tcgcctcgag
     7501 atcctttgcc gccgcgtgga gcgctaccgc gccaatgttt cccccggccc ctttgatggc
     7561 atgcacgagt ttgcgggccg cttcgagatc gcctacctcc atgaagcgtg cgagatcatc
     7621 ggcggcatgt tcgaactcgg cgcaaaaatc gaggatgacc gaccgaagca atgcggcatt
     7681 gccgccgagc cgatacaggc cgtctgcaag gtcgatcccg tgcggcgttt cgggaagatc
     7741 ggtccccgac gataattccg gaggcgaaat ccccccgcga tccgccacgc tcccgggaag
     7801 ccacttggtg atcatggcga caagctccgg cggatcgatg ggctttgcga tatggtcgtt
     7861 catgcccgcg ctcaggcatc gatcccgatc gcgctgcatg gcatgcgccg tcatggcgac
     7921 gattggaata tgggccagca tcgggtcctc gcggatgata cgcgtcgcct cgaagccgtc
     7981 catctccggc atctgcaggt ccatgagcac caggtcgaaa cggcttttcc gaagcatgtc
     8041 gatcgcctca cggccattac ccgtcgggac gaccgtcatt ccggcgccct tcagcacctc
     8101 catcgccacc agctgattga tcagattgtc ctcaaccagg aggatccggc ggtcggccgt
     8161 cgcatcggtc ttgaccgccg cccggtgctc gatgcgcgcc cgagtgacgt caatcggacc
     8221 gatcgcgcca tcaccccaca caccgcccgc gcacacgccg agcggaacat cgagagagaa
     8281 cctgctcccc tcgccgggcc ggctctccac gacgatggag ccgcccatca gctcgacgag
     8341 gtttcgcaca atcgcaagac ccagtcccgt cccgccaaat cgccgggtaa tggacgcatc
     8401 cgcctgggtg aacggcttga aaatgcgttc ccgctgatcg agttcgatgc cgatccccgt
     8461 gtcctccacg ctgaagcgaa cgacagcgcg atcgggaccg ccggggccag gcaggcgcga
     8521 tatgcgcacg cccacgtagc cccgctcggt gaatttgatc gcattgccga tgagattgcc
     8581 gagaacctgt tgtatgcgca gcgggtcccc ccgcaggcag accggaacgt ccggatcgac
     8641 ctgcatgcga aactcaacgc ccttgtcctc gaccgtgcgg gcgaatagat tggacagggc
     8701 gcccagcagc gtcgcgaggt cgaaatcgac aatctcgaga tcgagccgtc ccgcctcgat
     8761 tttggaaaaa tcaagaatat tgttgatgat gcccagcagc agctgcgcgg aatcctgaat
     8821 cttgccgatg taatcgcgct gtcgttccgt gagctccgtc tgttgaatca gatgggtgag
     8881 gccgacaatc gggttcatgg gcgtgcggat ctcgtggctc atgttggcca ggaactcgct
     8941 cttcgccttc gccgccgcct gcgcgttctc gagcgcccgc gcgagcgcca tatccacccg
     9001 ttttttttcg atgatcttcc agacgccttc cagatacagc gtcagctgat ggacatcgag
     9061 ctggctatag gggtccggct tcccggcgca tccgagaacg ataaccgcct ccccgttctt
     9121 cacgtgggcg acgctcatca cgcgaatggg ggccggtgcg gcatcgccgc ccgtcatggc
     9181 gaaaccgcgc tcgtggatga tgggcgcgag cgcccatgcg ctctccgccc accagtcggc
     9241 cttccccggt tgccggagcg cgtccatgtc gtccaacaca atcgcatcgg gattgccgca
     9301 tgaagcggca tgctcggcat cgccgacgtc ggcgccccgc acgaccatgc ggccgatatc
     9361 gcttgcgaca aggggaagcg cctcggcgag cgctcggtcg accagctctt gctcggtcgc
     9421 gccttcgccc tggttgagcc ggtagagcgc ttccagccgc gcctcattga gtcgcatctc
     9481 gtcctcggcc tggcggcgat cggtgatgtc gcgcaccagc accacgcggg cgatcaccgc
     9541 cccgctccgg tcgtgcagcg gagaaattcg cacatcgaag tgccgcagac catcgcccag
     9601 cggcaatgac agctccgatt ggatgctcga caccctgcgg aagcgctgca cgaggtcacc
     9661 gaac
//
LOCUS       DAORHF010000136         9659 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6434_length_9659_cov_4.676905, whole genome shotgun sequence.
ACCESSION   DAORHF010000136 DAORHF010000000
VERSION     DAORHF010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6434_length_9659_cov_4.676905"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1169
                     /locus_tag="PKJ16_11660"
     CDS             339..1169
                     /locus_tag="PKJ16_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV47689.1"
                     /translation="MIQWFSETRKHSDYELEYRFRHKDGTYRWFLAKAALIFDEDSKP
                     IRLLGSHIDITHLKKTEESVRELERQLIDISEQERRLIGSEIHDDLGQKLTYANLLFD
                     IIKKEIIPPRFKQHKDVITMKRLLGEIIDQTRALAYGLNPVRLEQGGFLAALEDLAVE
                     TARITGIDVPLAVTGTISIGDNTVATNLYYIIKEAVTNAIKHAKGNGLAIEIAGDTTW
                     ITTRVINRIASDAKRSVGAKSGLGLSHMQYRAHTIGAYLNILQAADRFEVIVELRNNN
                     "
     gene            1248..1919
                     /locus_tag="PKJ16_11665"
     CDS             1248..1919
                     /locus_tag="PKJ16_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV47690.1"
                     /translation="MKNAPISIYLVEDHPLVRQGVLRILEREKDFEICGEADNANVAI
                     VQIAQREPDIVILDITLEGEANGIDLIKSIRARGLKTLPIVLSMHAEALFIERALRAG
                     ARAYINKKELGEAVVVAIREVLKGELYLSASASSRIINRFLSSGESHIARDEVHSMGD
                     LTDRELQVFELIGQGMKTGDIARTLNISTNTIEAHRRNIKHKLGLSSGSELARNATQW
                     VIQNR"
     gene            2226..3947
                     /locus_tag="PKJ16_11670"
     CDS             2226..3947
                     /locus_tag="PKJ16_11670"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47691.1"
                     /translation="MAWYNNIKIMTKLLSGFVFVAIMAGIVGVVGILEMRAIDKADTF
                     MYEKMTLGLNYVGKLGISYNRMRAELRALPLASTADVRRESFETIGKLRAEFEEVMKL
                     YTNTFIDENDKKQYDELVKRYNDYIPLAEKVIRLTETGKMAEAESILLGEGLKVARDL
                     QDKLQAMLAANEKAARETSEKNTALANRATVIMIATILIAMAVAILLGFSISLSIKRP
                     VEKAMQFSRMISQGDFTGRIDLDQKDELGQLGASLNKAADSLEDLVANLVTASQNLAQ
                     AVEQISSGNQNLSQRTSEQASSLEEVASTIEEATANIKQNAENAIEANKLAGNSTKLA
                     EDGGRITDEAVASINEINLASKKIGEITAVINEISFQTNLLALNAAVEAARAGEQGRG
                     FAVVASEIRNLAQRSGNAAKEIADMIKDTVEKVANGTLLVNNSGESLKEIIKGVRDLN
                     RFITEIAAASDEQMRGMDQINVAISEMDTMTQQNAALVEETASASEEMSNQAQELLMM
                     TEKFTIRNETGRNKHHELHLRAANESLPGKSIGKLSHNKGNGNSGTERTVDTIKKALE
                     RDGFEEF"
     gene            4737..4928
                     /locus_tag="PKJ16_11675"
     CDS             4737..4928
                     /locus_tag="PKJ16_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HNV47692.1"
                     /translation="MFFYIPKIDESAVYRYTHDVSLSVAINTYLSLSVGYLHEFNNNP
                     APGVKNIDETINAQVSINL"
     gene            complement(4951..7002)
                     /locus_tag="PKJ16_11680"
     CDS             complement(4951..7002)
                     /locus_tag="PKJ16_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV47693.1"
                     /translation="MVIAVVVWEPAYAMKPVVLDDDATVHTLGLYADVLEDPKGTVTL
                     ESILRGDHDARFVPSASDAPNYGFTTSVYWARFQIRNVRDVPMERFLEIAYPILNHAE
                     LHVPEPGGGYTVRIGGNSYPFGKRDLQYRNLVFKIDIRPGDQVLYLRIKTQSSMCFPL
                     ILQSPTSFSETVLNEYIVFGLYYGILLVMIIYNLFVFLSVRDVNYVYYIVYILGYLLA
                     VLSHNGLAFQYLWPDSLFWARICVPFFILFVTMLAIPQFSRSFLKMSTYAPWIDRVLI
                     GQIAIGVGMMVATFFVRYSIIMPLTSVLAVISITISFIGGIVCLVRGYRAARFYVIAW
                     TVLTIGMALWFCQAVGILPYNFITKYSNQVGSAAEVVLLALALADRINILKKEKEEVQ
                     DELIGMQKSYSQSLEKTVQERTSELELKTRELESERNRLKRRNEMMEREVNLARRIQE
                     QLIPSHNPTGYIASLYRPMEQVGGDFFDFIMFDDSDRIGIFLSDVSGHGVPAAFITSM
                     VKTIILEAGPRNENPAALLSYINAILFRQTGGNFITAYYGIYDRGDRTILYANAGHFP
                     PFIIAGGAISTLMGRSSVPLATLHNKYLEDAGKSYVNNVVQLPDAGKVLLYTDGFTEA
                     QPVEAPGRFFEYNGMAEALTTHAHLPSREFLEQVFNRLIEYRGEETFDDDVCLICLDV
                     S"
     gene            complement(7167..7433)
                     /locus_tag="PKJ16_11685"
     CDS             complement(7167..7433)
                     /locus_tag="PKJ16_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47694.1"
                     /translation="MNEEKRIATEDNTPKDGDEMNERRTVDRRSCAGRRRFSRRLYKT
                     NIFTDDLFGEKAIVDKRKMKDRRMRDRRRTSSQRPSKSVPPTQK"
     gene            complement(7541..8158)
                     /locus_tag="PKJ16_11690"
     CDS             complement(7541..8158)
                     /locus_tag="PKJ16_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HNV47695.1"
                     /translation="MIRVRRLLILLVFLGASCSSNYEMMVKSYHDPETDFGKFRTFAV
                     LPMTKGNPSLQRELLFQVKITMVNRGYKYNPVKPDFLIAMDLLTADYTPPGAIKIKTL
                     PSVDETGKPVGPEPLIIRSAGPKPDAESGSDNAAFRTIVLSIADNRRPAGGAAAVVWE
                     GVAQTPPAADRLDDVTRRAVEELLRDFPGGRPGGERRIPLERMNR"
     gene            8308..8517
                     /locus_tag="PKJ16_11695"
     CDS             8308..8517
                     /locus_tag="PKJ16_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47696.1"
                     /translation="MISRNNQEIPITPEEKLQKSADFCKDKCPVCTRARKKGRGFLYA
                     MVKFESKFCPHCKANAKVYGKPAYK"
     gene            complement(8557..8925)
                     /locus_tag="PKJ16_11700"
     CDS             complement(8557..8925)
                     /locus_tag="PKJ16_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47697.1"
                     /translation="MIDIKGIEEGVLIETRDFNMCIQNLYNDIKVGGTNLSDEEVRRF
                     TCNVIGSMVTEGLVRLVKTTYMQEDEDVYSYESERDLTPEETDLILKEPERWEEENVF
                     SPADVYELVITGKGRARIGS"
     gene            complement(9276..>9659)
                     /locus_tag="PKJ16_11705"
     CDS             complement(9276..>9659)
                     /locus_tag="PKJ16_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47698.1"
                     /translation="GMCSAEDTAPPVIRVPENGILQGWTVFPVDIPLAVDFQVRDDGQ
                     LLEVTGANLVEVNGNTRTYRIDLHSIQAVSGAGSYEKRITATDMCGKRTQYVFRYDNQ
                     LTGVLKVYYWLWWYQYTISYSFVPL"
BASE COUNT         2391 a         2581 c         2590 g         2097 t
ORIGIN      
        1 ggctacctgc acgagtttaa caacaatccc gcgcccggcg tgaagaacat cgacgagacg
       61 gttaacgcgc aggtgtcgat caatctttag ttgggcaaca actcagtacg gcgcgtggac
      121 cacattctga gcagtcctgt ataagtcagg aaatttctga tgtgatcata acctctattg
      181 aaccttgatc aataatcttt ccagaatttg tatcacaaaa aacaatcact atacgcaaat
      241 atagctaatt tgttatttaa acttaaaaat aaatcgataa ttattcttga aaaataatta
      301 ttttcagtga tttatcgatc ttacaaaatt taatattctt gatccaatgg ttttcggaaa
      361 caagaaaaca ttccgactat gagcttgagt accgctttag gcacaaggac ggcacttacc
      421 gctggtttct cgcaaaagcc gcattgattt tcgatgaaga cagcaaaccg atccgtctgc
      481 tcggaagcca cattgacatc actcacctca aaaaaaccga ggagtcggtt cgagagctcg
      541 agagacagct gattgatata tccgagcagg agcggcgcct gattggctca gaaatccacg
      601 acgatctggg tcagaagctt acctatgcca atctcctttt cgatataatc aagaaggaga
      661 taatccctcc tcggtttaaa caacacaagg acgtaataac catgaaaagg ttgctcggcg
      721 agatcatcga ccagacgcgc gctctcgcgt atggcctcaa tccggttcgg ctcgagcagg
      781 ggggatttct cgcagccctc gaagatttag ccgttgagac cgcccggata acgggcatcg
      841 acgtgccgct agcggtcacc ggtacgattt ctatcggcga caacaccgtc gccaccaatc
      901 tttactatat catcaaggaa gcggttacca acgccatcaa gcatgcgaaa ggaaatggtc
      961 tcgccatcga aatcgcggga gatacaactt ggattaccac tcgcgtaatc aaccgtatcg
     1021 cctccgacgc aaaacgttca gtaggtgcaa aatccggtct tggattatca catatgcagt
     1081 atcgagcgca tactatcggt gcgtatctaa acatcctgca ggccgcggac agattcgagg
     1141 ttattgtcga attaaggaac aataactaga atccatcatt gacgcatcgc gattattgtg
     1201 gtaagcatag agcgatagcg cacacgatcg taatagccga tacagcaatg aaaaatgcac
     1261 ccatcagtat ctacctcgtc gaagatcatc cgcttgtccg gcagggtgtt ctacgtattc
     1321 ttgaaagaga gaaggatttt gaaatatgcg gcgaagccga caatgcgaat gtggcgatag
     1381 tacaaatagc gcaacgagag cctgatatcg tcattctcga tattaccctt gaaggggaag
     1441 cgaacgggat cgaccttata aagagcattc gagcccgcgg cctgaagacc ctgccgatcg
     1501 tgctttccat gcacgctgag gccctgttta ttgagcgagc cttacgcgcc ggagcccggg
     1561 cgtatataaa caagaaagag ctgggcgaag ccgtcgtagt tgcgatccgc gaggtgctga
     1621 agggtgagct ttacttaagc gcaagcgctt cctcgcgtat tatcaatcgg ttcttgtcct
     1681 cgggagaatc gcatattgcc agggacgagg tgcactctat gggcgatctc acggaccgtg
     1741 agctacaggt cttcgagctc atcggccaag gaatgaagac cggcgatatc gctcgcacgc
     1801 tcaacatcag cacgaacacc atcgaagcgc accgccgcaa catcaaacat aagctgggcc
     1861 tttccagcgg cagcgagctg gccagaaacg caacccagtg ggtgatacag aaccgatagc
     1921 ccctctccgg aattcattaa tggattcctt tacgctgtag tagctaaaat ccgcgattac
     1981 cacaaaccat gattattgtt attatcacaa tggcatgctg attgccgcat gtgtaacttc
     2041 gcaaggattt cggcacgatt tgacgagacc catgtcgatt aaaagttttc atcatcgata
     2101 tttgcgatct taaccgtgct ggacacaatg ccgggagccg tatttcaccg acacgagggc
     2161 agtgtctggc tattggcatg cgattacaac actggcaata acagtataat tagcggaggc
     2221 gcaccatggc atggtataac aacatcaaaa taatgacaaa gcttctaagc ggttttgttt
     2281 tcgtggccat tatggccggc attgtcggcg tggtcgggat tttagagatg cgtgcaatcg
     2341 acaaggccga cacattcatg tacgagaaga tgaccctggg cctcaattac gtcggcaaac
     2401 ttggcatttc gtacaaccgg atgcgcgccg aactgcgcgc gctcccgttg gcgagcaccg
     2461 cagacgtgcg tcgcgaatcg ttcgagacga tcgggaagct tcgcgccgag ttcgaggagg
     2521 ttatgaaact ctacactaac accttcatcg atgagaacga caagaaacag tatgacgaac
     2581 tggtcaagcg atacaatgat tacatacctc tcgccgaaaa ggtgatccgt ttgacggaaa
     2641 ccggcaaaat ggcggaagcg gagtcgatat tgcttggcga aggcctcaag gtcgctcgcg
     2701 atttacagga caagctccaa gccatgctgg ccgcaaatga gaaggcggca cgcgaaacat
     2761 cggaaaaaaa tacagcgctt gcgaacagag cgacggttat catgattgcc acaattctca
     2821 tcgccatggc ggtggccata ttattgggct tctcgatctc tctttctatc aagcgtccgg
     2881 ttgagaaggc aatgcagttc tctcggatga tctcacaggg ggattttacc ggaaggatcg
     2941 atcttgacca gaaggacgag ctgggccagc tcggcgcatc gctcaacaag gcggctgata
     3001 gcctcgagga tctcgtcgcc aacctggtaa ccgcatccca aaacctcgcc caggcagtcg
     3061 agcagatttc aagcggaaac cagaacctct cgcagcgaac gagcgaacaa gcctcatctc
     3121 tggaggaggt ggcgtccacc atcgaggagg cgacggccaa catcaagcag aacgccgaga
     3181 atgcgattga agcgaacaaa ctcgctggaa attcaacaaa acttgccgaa gacggggggc
     3241 ggataaccga cgaagcggtc gcttcgatca acgagattaa cctggctagc aaaaaaatcg
     3301 gcgagatcac ggcagtaatt aatgagatat catttcagac gaatcttctc gcgctcaatg
     3361 ccgcggttga ggctgcgcgc gccggtgagc aggggcgcgg tttcgcggtt gtcgcgagcg
     3421 aaatccgcaa tctcgcgcag cggtctggta acgccgcaaa ggagattgcc gacatgatca
     3481 aggatactgt cgagaaggtc gctaacggca ccttgctggt taataatagc ggcgaatctt
     3541 taaaagaaat aatcaaaggt gtccgcgatt tgaatcgctt tatcacggag atagcggctg
     3601 caagcgacga acagatgcgc ggcatggacc agataaacgt ggcgatatca gagatggata
     3661 cgatgacgca acagaatgcg gcgcttgtgg aggaaaccgc ttcggcaagc gaggagatgt
     3721 cgaaccaggc gcaggagctt ctcatgatga ccgaaaagtt cactatccgg aatgaaaccg
     3781 gacggaataa gcatcatgaa ttgcatctac gcgcggctaa cgaatcacta ccaggtaaat
     3841 cgatcgggaa actgtctcat aataagggga acggaaattc aggtacggaa cgcacggttg
     3901 acacaatcaa aaaagccctt gaacgggacg gattcgaaga attctgaacg atgacgattc
     3961 tccacgagat cgtaaataaa ggacgagatg ggaggatttt cagtgaatag accgggtagt
     4021 atagaacgaa tgaaaaccat tgcgagaagc ctcacagcga ttaatgggcg tacgtttcgg
     4081 ggttgtgttc atagccctga gataaaagac aatgcaagta aagaggctat catcgaatcg
     4141 ttacttgttc tacagaagtc gttcgacgag atatcgaagg aatgcgaagg aattgctgcc
     4201 ttcattgatc gtgaatttat caatacttcg aatattatgc attagcgcat acatcgacag
     4261 ttgtgatgca gcattcaatg atgtgcgcaa tacactcttg atgtcgatac tttgcttcag
     4321 ttctccggtc tgattgccgt tatgttcaat ctacgatgac atatccgcat agttaaagga
     4381 gcagccgtat ataccggacg caccatgaag aaaccccggc cgaaggccgg ggtttctgtg
     4441 ttaaagtgtc aatgattggt cgatagaaac cagtatttga attctccaaa gacaccgaca
     4501 tggccaatgt cgcccagccc cagcaccgct tgtatctgat tcgccaaaca ttgagaagct
     4561 tgattgagcc atgggattga attaactggt gtagccgtat agtttcggca ctaccgccgc
     4621 ctatcgtgct ggcgtgacgc cgcccgcatc gtcgtttacc tggaccgata tcttcgcctt
     4681 cgttacgccg gtcaaggcgg gacgcgacgg accagtggcg atggtcgctc cgctacgtgt
     4741 tcttctacat tccgaagatc gacgagagcg cggtgtatcg ctacacgcac gacgtgagcc
     4801 tctcggtcgc gatcaacaca tacctgagcc tgtcggtggg ctacctgcac gagtttaaca
     4861 acaacccggc gcccggcgtg aagaacatcg acgagacgat taacgcgcag gtgtcgatca
     4921 atctataatg cggccgttcg cggcgccctg tcaggaaacg tcaaggcata tcaggcatac
     4981 gtcatcgtcg aacgtttctt cgccccggta ctcgatgaga cggttgaaca cctgttcaag
     5041 gaactcgcgg ctcggcaggt gcgcatgggt agtaagcgcc tccgccattc cgttgtattc
     5101 gaaaaatcgt ccgggggctt cgaccggctg ggcctcggtg aaaccgtcgg tatacagaag
     5161 gaccttgccg gcgtcgggga gctgcaccac attgttcacg tacgatttcc ccgcatcctc
     5221 caggtacttg ttgtgcaggg tcgcaagcgg tacgctcgag cgtcccatca gtgtcgatat
     5281 cgcgccgccg gcgatgatga atggcgggaa gtggccggca ttcgcataga ggatcgtccg
     5341 gtccccccgg tcgtagatgc cataatacgc ggtgatgaag ttcccgcccg tctgccgaaa
     5401 gagtatggcg ttgatgtacg acaggagcgc ggccggattt tcgttccgcg ggccggcctc
     5461 gaggatgatc gttttcacca ttgaggtgat gaaagcggcc ggtacgccgt gtcccgagac
     5521 gtcgctcagg aagatgccga tgcggtcgga atcgtcgaac atgatgaaat cgaagaaatc
     5581 gccgccgacc tgctccatcg ggcgatacag cgatgcgatg tatcccgtcg ggttatgcga
     5641 gggaatgagc tgctcctgga tccgccgcgc gagattgacc tcccgctcca tcatctcgtt
     5701 gcgccgtttc agccggttcc gctccgactc gagctcccgc gtcttcagtt ccagctcgct
     5761 ggtgcgttcc tgtacggtct tctccagcga ttggctgtag gatttctgca tgccgatcag
     5821 ctcgtcctgc acctcctcct tctctttttt cagaatgttg atacggtcgg cgagcgcgag
     5881 cgcaagcagg accacctcgg cggccgaacc gacctggttg ctgtacttgg tgatgaaatt
     5941 atacggcagg atcccgaccg cctgacagaa ccagagcgcc atcccaatcg tgagcaccgt
     6001 ccaggcgatg acatagaagc gcgcggcgcg gtatccccgg acgaggcaga cgatcccccc
     6061 gatgaacgag atggtgatcg agatgaccgc gagcaccgag gtgagcggca tgatgatcga
     6121 atagcggacg aagaacgtcg cgaccatcat cccgacgccg atggcgatct ggccgatcag
     6181 gacccggtcg atccacggcg cgtatgtgct catcttgagg aacgagcggc taaactgcgg
     6241 aatcgccagc atcgtcacga acaggatgaa gaaggggacg cagatccgcg cccagaacag
     6301 cgagtccggc cacaggtact ggaaggccag cccgttatgc gacagcaccg cgagcaggta
     6361 cccgaggata tacacgatgt aatacacgta attgacgtcc ctgaccgaca ggaacacgaa
     6421 cagattgtag atgatcatga ccagcaggat gccgtagtac aacccgaaca cgatgtattc
     6481 gttcaggacg gtttccgaga acgaggtggg gctttgcagg atgagcggaa aacacatgga
     6541 cgactgggtc ttgatcctca gatacaagac ctggtccccc gggcggatat ctatcttaaa
     6601 caccaggttt cggtactgca ggtcgcgctt cccgaacggg tagctgttcc ctccgatacg
     6661 gacggtgtac ccgcccccgg gctccggaac atgcagctcc gcatgattga ggatcgggta
     6721 tgcgatctcg aggaaccgct ccatcggaac gtcacgcacg tttcggatct ggaatcgcgc
     6781 ccagtacacg gatgtggtga agccgtagtt gggagcgtcg gatgcgctcg gtacgaatcg
     6841 cgcatcatgg tcccctcgaa ggatgctctc cagcgtgacg gtccccttcg ggtcctcgag
     6901 aacatcggcg tacaaaccca gcgtgtggac cgtagcgtca tcatcgagca ccacgggctt
     6961 catcgcatat gcgggctccc acacgacgac agcgatgacc atcgcaatca acactgccgt
     7021 tgtcggtatc aatttcacgt gtttccaggt ccgtgtcgcg cgcacacagg agtaccacat
     7081 gcgataccgc accgtcaagg agttttctct gatgggggtg atgggtgatg cgcgcggcgg
     7141 cggaatgact ccccgacgct gcgatcctac ttctgggtgg gcgggacgct cttcgaagga
     7201 cgctgtgacg atgtgcgccg ccggtcgcgc atgcggcggt ccttcatctt gcgcttgtcc
     7261 acgatggcct tctccccgaa cagatcgtcc gtgaagatgt tggtcttgta gagccgtcgg
     7321 ctgaaccgcc ggcggccggc gcacgatctc cgatcgaccg ttcgtcgttc attcatctcg
     7381 tcaccgtcct tcggggtgtt gtcttcggtc gcaatgcgtt tttcttcgtt catggcgcgc
     7441 accggatacg ggaaatgcca tgagcatact cgcccgtgcg aatacgcaat aaaaaaatga
     7501 aacaatgttt gcgaacggga aatgaccagc gcccgtggga ttatcggttc atccgctcca
     7561 gcggtattct ccgctcgccg cccgggcgac cgcccgggaa atcgcgcaag agctcctcaa
     7621 ccgcacggcg cgtaacgtca tcgagccgat ccgccgccgg gggcgtctgc gcaacgccct
     7681 cccacacgac cgccgctgcg ccgccggcgg gacgtcggtt gtcggcgatc gacagtacga
     7741 tcgttcggaa cgccgcgtta tccgatccgg actcggcgtc cggctttggc ccggcggacc
     7801 gtatgatgag gggctccgga ccgacgggtt tgcccgtctc gtcgaccgac gggagcgtct
     7861 tgatcttgat cgcgcccggc ggcgtgtaat cggccgtgag caggtccatg gcgatcagga
     7921 agtccggctt caccgggttg tacttgtagc cccggttgac catggtgatc ttcacctgga
     7981 agagcagctc ccgctgcagc gagggattgc ccttagtcat aggcagcacc gcgaaggtgc
     8041 gaaacttccc gaaatcggtc tcgggatcgt ggtacgattt caccatcatc tcgtagttgg
     8101 acgagcagga cgccccgaga aaaacaagca gtatcagcaa ccgtcgaaca cggatcataa
     8161 ccgcacctcg cctgtgatcg tttataatag agacgatgtt cacttgaccc gggcaagaaa
     8221 aatatcatat ctagaaatga aaaaaatcca cccgagcctc gtgagatatc gattcacaga
     8281 aacaacctga tctttcacga tgccccggtg atttcacgca ataatcagga gatacccata
     8341 acaccggaag aaaagcttca gaaaagcgct gatttctgca aggacaaatg ccccgtctgt
     8401 acgcgggcac ggaaaaaggg gcgggggttc ctgtacgcca tggtcaagtt cgaaagcaag
     8461 ttctgcccgc actgcaaggc caacgcgaag gtgtacggga agccggcgta taagtagggt
     8521 ggggcgaagg gcggaaacga cagggcgcgc atgtgctcac gatccgatcc gcgctcttcc
     8581 ttttcctgtg atgacgagct catagacgtc ggcgggagag aagacgttct cctcctccca
     8641 tcgctccggc tccttcagaa tgaggtcggt ttcttcaggc gtgagatcgc gctcggactc
     8701 gtaggaatac acatcctcat cttcctgcat gtaagtggtc ttcacgagcc gcacgagccc
     8761 ttcggtcacc atcgagccaa ttacgttaca cgtgaaccgg cgcacttcct catccgagag
     8821 gttcgttccg ccgaccttga tgtcgttgta cagattctga atacacatgt tgaagtccct
     8881 tgtctcgatc agcacgccct cttcgattcc cttgatgtcg atcatttttt cgctccttga
     8941 gataccattg tcgctcccat cgcaatgcgg gaatgcgata ctaccctata tccataatca
     9001 gttctctgtc gatggatata ttaggtatac gaaggagcgt gaatacagtt aaacggtttt
     9061 tctcctagaa gccaattccc aacggtaagc cgcgatgatg cgcataacgg aagcactctt
     9121 accataacca gcatctgttt tattgctctc atttcctggc ttgcatatcc atttgttgtg
     9181 cgtttagagt acactgaaga ttcaaccagc gggtagtgat gaagcgccgg ttgaatcttc
     9241 tgattacaca acgtacctat tcgcatgaaa ctccactaca gcggcacgaa gctgtagctg
     9301 atggtgtact gataccacca caaccagtag tagactttaa gtacgccggt caactgattg
     9361 tcatatcgaa acacatactg ggtccttttt ccgcacatgt cggttgccgt tattcttttc
     9421 tcgtaacttc cagccccgga cactgcttgt atcgaatgca agtcaatgcg atatgtacgc
     9481 gtattaccat ttacctccac cagattggct ccggtgacct cgaggagctg cccatcgtct
     9541 cgtacctgga aatcaacggc aagcggtata tcaaccggga aaaccgtcca cccttgcaaa
     9601 atgccgtttt ccggtacgcg tataacgggc ggcgccgtat cctccgccga gcacatacc
//
LOCUS       DAORHF010000137         9625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6467_length_9625_cov_5.883072, whole genome shotgun sequence.
ACCESSION   DAORHF010000137 DAORHF010000000
VERSION     DAORHF010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9625
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6467_length_9625_cov_5.883072"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PKJ16_11710"
     CDS             complement(<1..419)
                     /locus_tag="PKJ16_11710"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HNV47699.1"
                     /translation="MSCRVIIGTQWGDEGKAKIIDYFTAGADFIVRYQGGANAGHTVV
                     VNGKKFVFHLVPSGMLHPGKTCVIGNGVVVDPEQLLREIAVLEKEGYEVRSNLLISDA
                     AHLILPYHKALDEAMEELRTNKIGTTKRGIGPAYADKA"
     gene            complement(517..1143)
                     /locus_tag="PKJ16_11715"
     CDS             complement(517..1143)
                     /locus_tag="PKJ16_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47700.1"
                     /translation="MKRTMISFFSVLMLLPVVLVGQEPKKTDKFNFPSHSIIDPRFSV
                     KNVTFNNVTDTTGTGETLQVEMELKNNVNDPHSLYVFVFATFEKDPYDDHIMRRPKPL
                     PDPMVYFTAVPADPKNYEYPKENGSGVHFVKFPRDPKLGVDPSTKKPYQLTDNLFIKT
                     KHHSKHRIGYVYFNTVTILVFDEKKLTDEKGQPWPVFRKVYAITKKRR"
     gene            1415..2506
                     /locus_tag="PKJ16_11720"
     CDS             1415..2506
                     /locus_tag="PKJ16_11720"
                     /inference="COORDINATES: protein motif:HMM:NF024807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HNV47701.1"
                     /translation="MTIALCLAVSACTDNFVDVENKEADEKRQEEASTPEVAGSAPWR
                     LIASPATPRSGAMTAVIGQWLYIYGGEDYSGVRRDFFRYNLDTGESELLPLGDYRAHG
                     TMTAVGGRLYVFGGYDNTGADITKSNGQLRYFDTVTNTWTSLGTADTNGTFPDERHGH
                     VAAYYRIPEEGRDKLYFQGGFTGTGNASNGVIVLDLDDPPKPTWSTRATGPTRGHSGA
                     AIISGRLYVFGGYTKSNTGDTYSNELYEYRITANTWSLVGSLSGATGRRAGTAVVAIG
                     ANLYLYGGTAPGNHYGDLWRFNTSLPPFAATSPWRQLTAGPSGRCNMGVAVFDDRMHV
                     FGGHSENDLGQGLYTAELWEYDPEREPVD"
     gene            2621..3658
                     /locus_tag="PKJ16_11725"
     CDS             2621..3658
                     /locus_tag="PKJ16_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47702.1"
                     /translation="MRTSSALCAVLALVLAFARTDTALPSDLPADIMFGVKGGYSQVI
                     GHYADFVHGAGGGGVFVMPYIGSFFMIEADLSYASYALARERGSSLNLASGGIGPLFY
                     VQPFSFLQIYVGASITGNYFALNASRERGEQESMKAGFAGKFGFIFPFRNGLAARIGC
                     EYAQAFQSGNAFAAVNYYGGVAYGVSFWNAPPAVSEAETAFAKGQREFKEGRFDEAKR
                     SFAKTRSLDGSHAGAKQHLDTIERLERQYATARDLIAKHRFYEAIPVLDDIAAEYPAA
                     REDLAKARAQLAGEIPTLARQGIAAYERQDYRTCIALMQRIILIDPKNETANIYLPRA
                     RKRLEALERLR"
     gene            3669..6419
                     /locus_tag="PKJ16_11730"
     CDS             3669..6419
                     /locus_tag="PKJ16_11730"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47703.1"
                     /translation="MKIKYKLILVLAVVILVASLPLSLFILGEQENEKIALISRQGAL
                     NSRILARSAMNILLMNGGNVSVAQVDARDMMGMFAPLTEHGLVLADAVLVSARQDYNG
                     ALLARMRTGAPGARTDDKRSLAPEELERLVRHPGFAEAHPPGIDEDCYEFVSASDPAR
                     RGAYCIGRLVFSKRVVLAPIRRLRLIIYGATAFSIGLVCLLGFALSRFISRPIDTLTE
                     GLRKIEIGDLDSQLPVRGHDELSRLATTFNHLLRMINLQMGSLIASNRELKRLDQLKD
                     EFLANMTHELRTPLAGMIGLAEALQKGAAGELPDAARHDLTLIEAGGRRLSGLVNDIL
                     DYSKLKYRDIELSPGPVDMHSVAQSVLAVLSALIAKKSLTVENRIAPGAAVVLGDKER
                     LLQVMMNLVGNAVKFTERGGIVIAAEDDPRDAEMAMIVVSDTGIGVPADKVDRIFEMF
                     EQADGSLSRSYGGTGIGLAITRKLIEMHGGRIWVDAVPGGGSRFSFTVRRSPRRATPE
                     GETAALPALPAITGEDVRSPAASARDAREEPPRRVLIVEDDPINMQVLVNYLALEGYD
                     ALTAETGPDALALLESAPAPDLVILDVMLPKLSGYDVCRRIRATYQHHDLPVLMLTAK
                     VRPEDVVAGIEAGANDYLPKPVSKEELLARVNNLISMKNAVREREELLNLKRDLHIAH
                     EIQQALLSQDLPRNERATVALRYLPMYELGGDFYSVGLADETKLTILIADVSGHGIAA
                     ALICAMLKVACSIHREEAQDPSRFFERVNATMYDHCYGQFVTACYIAVDLSARIMVQA
                     NAGHWPALVWKRNEGRMLADRPQSMPFGWVPEESFVSLTAALDPGDRIVLYTDGIIEA
                     RDYDNQMFGDRRFHDLIIRTQDSPPEEAADAIMRAVTEWSGKADGESLADDVTLVVID
                     VA"
     gene            complement(6549..7226)
                     /locus_tag="PKJ16_11735"
     CDS             complement(6549..7226)
                     /locus_tag="PKJ16_11735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HNV47704.1"
                     /translation="MKNMKHAVFALILLGALPVTSCKASLDSDTAKVSYGLGMQFAND
                     MKNRGFEIDIKAFVQAVEDVYKGQKSRIADEEIRAAFQKVSEGMMQKQIAKAAENLKT
                     GEEYLTKNKAKDGVKVTASGLQYKVVKEGAGASPRPTDMVKVHYRGTLIDGTEFDSSY
                     KRNEPAEFRLNQVIPGWTEGLQLMKVGSKYELTIPAKLGYGEQGNQRIPGNSVLNFEV
                     ELLEIKK"
     gene            complement(7443..8522)
                     /locus_tag="PKJ16_11740"
     CDS             complement(7443..8522)
                     /locus_tag="PKJ16_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47705.1"
                     /translation="MSEAIYEKLIDVLDGLPNGFPRTPSGVEMKLLKRIFTAEEAELF
                     CHLRLHGESPAEISVRAGRPAEVLVPVLERMWEKGQILRDPRGSEQVYRLVPWIVGIY
                     EFQLPFMDKELAEMCLEYIRTLGPPLFMQKPQISQVVPIETEIRAGQEALPYERVSAI
                     IEKGKSFAVNECICKKQNDLTGRGCDKPREVCLAISEHENFFVDHPMKPRIITREETY
                     DILRMAEDAGLVHTTSNVREGHVFICNCCGCCCGQLIGARKGAPNIINSHYFARIDPE
                     KCTQCDTCIDTRCTIRAITKGEGRNVINEAKCIGCGLCVTKCPSKAITLIRKPDDQRM
                     EPPADEMEWYRARARARGVDISRYE"
     gene            8621..9172
                     /locus_tag="PKJ16_11745"
     CDS             8621..9172
                     /locus_tag="PKJ16_11745"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HNV47706.1"
                     /translation="MKILILNGNPDASDTGLDDYIAELGTQLAESGNDAELVTLREKK
                     IRPCTGCFGCWIKTPGTCSIPDDAIELAGRYLAADVVILASPLVNGYLSSLLKNAIDR
                     NIPIIHPYLEAVDGEVHHLKRYDRYPEIGILLQREPSTDEEDIEIVTDLFKRMIINMR
                     TTLKFLEFTDKPAPEVAHAVNIH"
     gene            9153..>9625
                     /locus_tag="PKJ16_11750"
     CDS             9153..>9625
                     /locus_tag="PKJ16_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47707.1"
                     /translation="MQLTFINGSPRGAKGNTARLMDNFVRGFLETPGASCAVEYVAKH
                     RTDLAGLKKIFYASENVIIGFPLYVDAMPGSVKEFLDVLIPPPQGAAVPALGFMSQCG
                     FPETGHLRFVERYLEKYTKRLGASYLGSIMKGGGEGLHMQPKFLVDKFENGLHSLG"
BASE COUNT         1709 a         3041 c         3002 g         1873 t
ORIGIN      
        1 gccttgtcgg cgtaggccgg gccgatgccg cgcttggtgg tgccgatctt gttggtgcgc
       61 aattcctcca tcgcctcgtc gagcgcctta tgatagggga ggatcaggtg cgcggcgtcg
      121 gagatcagga ggttcgagcg gacctcgtag ccttccttct cgagcacggc gatttcgcgc
      181 agcagctgct cgggatcgac caccacgccg ttgccgatca cgcaggtttt gccgggatgc
      241 agcatgccgg agggcaccag gtgaaagacg aacttcttgc cgttgaccac gacggtgtgc
      301 ccggcgttcg ccccgccctg atagcggacg atgaagtcgg ccccggccgt gaaataatcg
      361 atgatcttgg ccttgccctc gtcgccccac tgtgtgccga tgatgaccct gcagctcatg
      421 tcggtgtacc ccgcgaaatg gtgtatgatg cgcgcgcgca ggaagccatc acatcaataa
      481 aaaccataca ccacaagcct ttttcgcgcg tggccgctag cggcgttttt tggtgatcgc
      541 gtacaccttg cgaaacaccg gccacggctg gcccttctcg tccgtgagct ttttctcgtc
      601 gaagacgaga atcgtcacgg tgttgaagta gacatagccg atccggtgct tcgagtggtg
      661 cttcgtcttg atgaagagat tgtcggtgag ctgatagggc ttcttggtcg acggatcgac
      721 ccccagtttg ggatcgcggg ggaacttcac gaaatggacg cccgatccgt tttccttcgg
      781 gtactcgtag ttcttggggt ctgcggggac ggccgtgaaa tacaccatgg ggtccggcag
      841 cggcttgggg cgtctcatga tgtggtcgtc gtacgggtcc ttctcgaagg tcgcgaagac
      901 gaacacgtag aggctgtgcg gatcgttcac gttgttcttc agctccatct ccacctggag
      961 cgtctccccc gtcccggtgg tgtcggtcac attgttaaag gtgacgtttt tcaccgaaaa
     1021 tcgcggatcg atgatggaat ggctcgggaa attgaacttg tcggtttttt tcggctcctg
     1081 cccgacgagt acgaccggca gcagcatcag cactgaaaaa aatgatatca ttgtccgttt
     1141 catggcatcc tctggttacg ggagttcagt ttccttatcg gcgaaaaatc ctgttttcgt
     1201 taatagggaa ccggtattca atcttttgag aaaatattaa agaataatca aaaaaataaa
     1261 ttttcgaata aaaatatttg taataatgtt gatattttct ttgcagtagt ttaaattact
     1321 gtagcaagag tgggcttaat acgtccagac ggattttgtg atggagcgag tttacagata
     1381 tcttttgcca tcaacgaaaa aagcggcgct ttttgtgacg atagcgctgt gcctcgccgt
     1441 atcggcctgc acggacaact tcgtcgacgt tgagaacaag gaagcggacg aaaagcgaca
     1501 ggaagaggcc tctacgcccg aggtggccgg atcagcgccg tggcggctga tcgcgtcgcc
     1561 cgcgacgccg cgaagcggag cgatgacggc cgtcatcggg caatggctct acatctacgg
     1621 cggcgaggac tacagcggcg tgcggcgcga tttcttccgg tacaatctgg ataccggcga
     1681 gagcgagctc cttcctcttg gcgattatcg cgcccacggc accatgacgg ccgtcggcgg
     1741 gcgcctctac gtctttggcg gttacgacaa taccggcgcg gacatcacga aaagcaacgg
     1801 ccagctccgg tatttcgaca ccgttacgaa cacctggacc tcgctgggca cagccgatac
     1861 caacgggaca tttcctgacg agcgccacgg ccatgtcgcg gcatactatc gcattccgga
     1921 ggaggggcgc gataagctct atttccaggg aggtttcacc gggaccggga atgcgtcgaa
     1981 cggcgtgatt gtgctcgatc tggacgatcc gcccaagccc acctggagca cgagggcgac
     2041 cggtccgacg cgcgggcact ccggcgcggc gatcatcagt ggccgtttgt acgtctttgg
     2101 cggatacacc aagagcaata ccggcgacac ctattccaac gagctctacg aataccggat
     2161 aacggcgaat acctggtcgc tcgtggggtc tctctccggc gccaccggga gacgcgcggg
     2221 gacggccgtt gtcgccatcg gcgccaatct gtatctctat ggcggaaccg cgcccgggaa
     2281 tcattacggc gatctctggc gcttcaacac gtcactgccg ccgtttgccg ccacgagccc
     2341 ctggcgtcag ctcacggccg gaccgtccgg acgctgcaat atgggcgttg ccgtgtttga
     2401 cgaccggatg catgtctttg gcggccactc cgagaacgat ctcggccagg gcctgtacac
     2461 ggccgagctc tgggaatacg atccggagcg agagcccgtc gactgaccat ttcttttttc
     2521 ttcgttgacg ccgtttcgtt tccatgccta ccgtagcccc ctgttcgatt gcaaataacg
     2581 cggcatgcgc cgccgccgtt tattggggag ttactatcgt atgcgcacat catccgcgct
     2641 ctgtgccgtt ctcgctcttg ttctcgcatt cgcgcgaacc gataccgcgc tcccgtccga
     2701 tcttcccgcc gatatcatgt tcggcgtcaa gggcggctat tcgcaggtga tcggccacta
     2761 cgccgacttc gtccacggcg cgggcggcgg gggcgtgttc gtgatgccct acatcggttc
     2821 gttttttatg atagaggccg atctctccta cgcctcctac gcgctcgcgc gggagcgggg
     2881 atcgtcgctc aatctcgcct cgggggggat cggcccgctg ttctacgtgc agccgttctc
     2941 ctttctgcaa atctacgtcg gcgcgtccat caccgggaac tatttcgcgc tcaatgcaag
     3001 ccgcgagcgc ggggagcagg aatcgatgaa ggccgggttc gccggaaagt tcgggttcat
     3061 cttccccttc cgaaacgggc tcgccgcgcg tattggctgc gaatacgcgc aggcgttcca
     3121 atcgggaaac gcgttcgccg ccgtcaatta ctacggaggc gtggcctacg gcgtgtcctt
     3181 ctggaacgcg ccgccggccg tgagcgaggc cgagacggca ttcgcgaagg gacagcgcga
     3241 attcaaggag gggcgcttcg acgaggcgaa gcggagtttc gcgaaaaccc gctcgctcga
     3301 cggctcccac gcgggcgcga agcagcacct cgacacgatc gagcgcctgg agcggcagta
     3361 cgccaccgcg agggacctca tcgcaaagca tcggttctat gaggccatcc ccgtcctcga
     3421 cgacatcgcc gcggagtacc cggcggcgcg cgaggacctc gcgaaggcgc gcgcacagct
     3481 cgccggggag atccccacgc tggcgcgcca ggggatcgcc gcctatgaac ggcaggacta
     3541 tcggacctgc atcgcgctca tgcagcgcat catcctcatc gacccgaaaa acgaaacggc
     3601 gaacatctat ctgccgcgcg cgcgcaagcg tctcgaggct ttggagcgcc tgcgataggg
     3661 agcgtggcgt gaagatcaag tacaagctga tcctcgtcct ggcggtggtg attctggtcg
     3721 cctcgctgcc gctgtcgctg ttcatcctcg gcgagcagga gaacgaaaag atcgcgctca
     3781 tctcgcgaca gggcgcgctc aacagccgca tcctggcgcg ctcggcgatg aacatcctcc
     3841 tcatgaacgg cggcaatgtc tccgtggccc aggtggacgc gcgggacatg atgggcatgt
     3901 tcgcgccgct tacggagcac ggcctggtgc tcgccgacgc cgtgctcgtg tccgcgcggc
     3961 aggactacaa cggcgcactg ctcgcgcgca tgcgaacggg cgcgcccggc gcgcggaccg
     4021 atgataagcg ctccctcgcg cccgaagagc tcgagcgcct ggtgcgccat ccgggatttg
     4081 ccgaggccca tccgcccgga atcgacgagg actgctatga gttcgtatcg gcgagcgacc
     4141 cggcgcgtcg gggggcctac tgcatcggcc ggctggtgtt ctccaagcgc gtggtgctcg
     4201 cgcccatacg ccggctgcgc ctcatcatct acggcgccac ggccttctcc atcgggctgg
     4261 tctgcctcct cgggttcgcg ctcagccggt tcatctcgcg ccccatcgac accctcaccg
     4321 agggcttgcg gaagatcgag atcggcgacc tggactcgca gcttccggtg cgcgggcacg
     4381 acgagctctc gcgcctggcg accaccttca accacctgtt gcgcatgatc aacctgcaga
     4441 tgggaagcct catcgcctcc aaccgggagt tgaagcgcct ggaccagctc aaggacgagt
     4501 ttctggcgaa catgacgcac gagctgcgca cgcccctggc cgggatgatc ggcctggccg
     4561 aggcgctcca gaagggcgcc gcgggcgagc tccccgacgc ggcccggcac gatcttacgc
     4621 ttatcgaggc gggggggagg cgcctctccg ggctggtgaa cgacatcctg gactactcca
     4681 agctcaagta ccgggacatc gagctttcgc ccggccccgt ggacatgcac tcggtcgcgc
     4741 agtcggtgct tgcggtttta agcgcgctta tcgcgaaaaa atcgctcacg gtcgaaaatc
     4801 gcatcgcgcc cggcgcggcc gtggtcctgg gcgacaagga acggctgctg caggtgatga
     4861 tgaacctggt gggcaatgcg gtcaagttca ccgagcgcgg cggcatcgtg atcgcggccg
     4921 aggacgatcc gcgcgacgcc gagatggcga tgatcgtcgt gagcgacacc gggatcggcg
     4981 ttcccgccga caaggtcgac cgcattttcg agatgttcga acaggccgac ggctccctct
     5041 cgcgcagcta cggcgggacc gggatcggcc tggccatcac ccggaagctc atcgaaatgc
     5101 atggcggccg catttgggtc gatgccgttc cgggcggggg atcgcgcttc tcgttcaccg
     5161 ttcgccgctc cccccggcgc gcgacgcccg agggcgagac cgccgcgctg ccggcgctcc
     5221 ccgccatcac cggcgaggac gtgcgcagcc ccgccgcgtc ggcccgcgat gcgcgcgagg
     5281 agccgccgcg acgcgtgctc atcgtcgagg acgaccccat caacatgcag gtgctggtca
     5341 attacctcgc gctggaggga tacgacgccc tgaccgccga gaccggtccc gacgcgctcg
     5401 cgctgctcga gagcgccccc gcgcccgacc tggtgatcct ggacgtgatg ctcccgaagc
     5461 tgtccggcta cgacgtgtgc cgccgcatcc gcgcgaccta ccagcaccac gacctcccgg
     5521 tgctcatgct caccgcgaag gtgcgcccgg aggacgtggt cgccgggatc gaggccgggg
     5581 cgaacgatta cctccccaag ccggtgagca aggaggagct gctcgcccgg gtgaacaatc
     5641 tcatctccat gaaaaacgcg gtgcgcgagc gcgaggagct gctcaacctg aagcgcgacc
     5701 tgcacatcgc ccacgagatc cagcaggcgc tcttgtcgca ggacctgccg cgcaacgagc
     5761 gcgcgaccgt cgcgctgcgc tatctcccca tgtacgagct cggcggcgac ttctacagcg
     5821 tgggcctcgc cgacgagacg aaactgacca tcctcatcgc cgacgtctcg ggccacggga
     5881 tcgccgccgc gctcatctgc gccatgctca aggtcgcctg ctccatccac cgagaggagg
     5941 cgcaggaccc gtcgcgtttt ttcgagcggg tcaacgcgac catgtacgac cactgctatg
     6001 ggcagttcgt gaccgcctgt tatatcgccg tggacctatc ggcgcgcatc atggttcagg
     6061 cgaacgccgg gcactggccc gcgctggtgt ggaagcggaa cgagggccgg atgctcgcgg
     6121 accggccgca gagcatgccc ttcggatggg tgcccgagga atcgttcgtg tccctgacgg
     6181 ccgcgctcga tccgggcgac cgcatcgtgc tgtacaccga cggcatcatc gaggcgcgcg
     6241 attacgacaa ccagatgttc ggcgaccggc ggttccacga cctcatcatc cggacgcagg
     6301 attcgccccc ggaggaggcc gccgacgcga tcatgcgcgc ggtgacggaa tggtcgggga
     6361 aggccgacgg cgagagcctg gccgacgacg tgacgcttgt cgtgatcgac gtggcgtgag
     6421 gatgtgcgcg gcgggtcttt aacgcgcgag aggcggccga aagccgcctc tcagggacgt
     6481 gtcccgggaa tcgccacgcc ggcgtgccgg atcaggcgtt tcccgataca gtgacgattg
     6541 cgtgtcgctt acttcttgat ctccaacagc tccacctcga agttcaggac cgagttgccg
     6601 gggatgcgct ggttgccctg ctcgccgtag ccgagcttgg cggggatggt cagctcgtac
     6661 ttcgagccca ccttcatgag ctgcaggccc tccgtccagc ccggaatcac ctgattgagg
     6721 cggaactcgg ccggctcatt gcgcttgtag gagctgtcga actcggtgcc gtcgatgagc
     6781 gtcccgcgat agtgcacctt gaccatgtcg gtgggccggg gagacgcgcc ggcgccctcc
     6841 ttgaccacct tgtactgcag gccgctcgcg gtcaccttca cgccgtcctt cgccttgttc
     6901 ttcgtgagat actcctcgcc cgtcttcagg ttctcggcgg ccttcgcgat ctgcttttgc
     6961 atcatgccct cgctcacctt ctggaaggcg gcgcggatct cttcgtcggc gatgcggctc
     7021 ttctgcccct tgtacacgtc ctcgaccgcc tggacgaagg ccttgatgtc gatctcgaac
     7081 ccgcggttct tcatgtcgtt cgcgaactgc atgcccagcc cgtagctcac ctttgcggtg
     7141 tcggaatcga gcgaggcctt gcatgaggtc acggggagcg ccccgaggag gatcagcgca
     7201 aaaacggcgt gtttcatgtt cttcattgga ttatcctttg ttgagagttc aaccggcacc
     7261 ggggcgccgc tcggcgttcg gtggatcgat gaagcggccc gcgcgggtga tgtcgccggt
     7321 gcgcccgcgt gaccgccggt tgttggagtg aacagaaccg gccgtacggg ccttctgtca
     7381 attaactttt cccggcgccc gtcggcggac gggcgcgatc gtcacggccg tgcggccacg
     7441 ccctattcgt accggctgat gtccacgccg cgcgcgcggg cccgggcgcg gtaccactcc
     7501 atctcgtcgg cgggcggttc catccgctgg tcgtcgggct tgcggatgag cgtgatggcc
     7561 ttcgacgggc acttcgtgac gcacaggccg cagccgatgc acttcgcctc gttgatcacg
     7621 ttgcggccct cgcccttcgt gatcgccctg atcgtgcagc gcgtgtcgat gcacgtgtcg
     7681 cactgcgtgc atttctccgg gtcgatgcgc gcgaagtagt gcgagttgat gatgttgggc
     7741 gcgcccttcc gcgccccgat gagctggccg cagcagcacc cgcaacagtt gcagatgaac
     7801 acgtggccct cgcgcacatt gctggtcgtg tgcaccagcc cggcgtcctc cgccatcctc
     7861 aggatgtcgt aggtctcctc ccgggtgatg atccgcggct tcatgggatg gtccacgaag
     7921 aaattctcgt gctcggagat cgccaggcac acctcgcgcg gcttgtcgca gccgcggccg
     7981 gtgaggtcgt tctgcttctt gcagatgcac tcgttcacgg cgaaggattt tcccttctcg
     8041 atgatggcgg agacgcgctc ataggggagc gcctcctggc cggcccgtat ctccgtctcg
     8101 atggggacca cctgcgagat ctgcggtttc tgcatgaata gcggcgggcc cagcgttcgg
     8161 atgtactcca ggcacatctc ggcgagttcc ttgtccatga acggaagctg gaactcgtag
     8221 atgccgacga tccacgggac cagccggtac acctgctcgc ttccccgcgg gtcgcgcagg
     8281 atctgcccct tctcccacat gcgctcaagg acgggaacga gcacctccgc cggtcggccg
     8341 gcccggaccg atatctccgc gggcgattcc ccatgcagcc tgagatggca gaagagctcg
     8401 gcctcctccg cggtgaagat ccgtttcagg agcttcatct ccacgccgct gggcgtgcgc
     8461 gggaagccgt tggggagacc gtcgagtaca tcgatcagtt tttcgtatat cgcctcgctc
     8521 atggtgattc tcctgcaaaa aagccttgca aaattacata acataacaat gttatgttac
     8581 aaagtatgat gtcaacgctt tttttctgga gaagaccttc atgaaaatat tgatcctgaa
     8641 cggaaatccc gacgcgagcg atacgggcct cgacgactat atcgccgagc tgggtacaca
     8701 gctggccgaa tccggcaatg acgccgagct ggtcaccctg cgagagaaga agatcaggcc
     8761 ctgcacgggc tgtttcggct gctggatcaa gacgccggga acctgcagta tccccgacga
     8821 cgcgattgag ctcgccggga ggtacctcgc cgctgatgtg gtcattctgg cgtcgccgct
     8881 cgtcaacggg tacctctcgt ccctgttgaa gaatgcgatc gaccggaata taccgatcat
     8941 ccatccgtac ctggaggcgg tggacggcga ggtgcatcat ctgaagcgct acgaccgcta
     9001 tccggagatc ggcattctgc tgcagcggga gccctccacg gatgaagagg atattgaaat
     9061 cgtgacggac ctgttcaagc gcatgatcat caacatgcgc acaacactga agttcctcga
     9121 attcaccgac aaacccgcac cggaggtggc acatgcagtt aacattcatt aatggctccc
     9181 cgcgcggggc gaagggcaac accgcgcggc tgatggacaa cttcgtgcgc ggattcctcg
     9241 agacgcccgg cgcctcctgc gcggtggaat acgtcgccaa acaccgaacc gaccttgcgg
     9301 gcctgaaaaa aatcttctat gcgtcggaga acgtgatcat cgggttcccc ctctatgtcg
     9361 acgccatgcc cggcagcgtg aaggaattcc tcgatgtgtt gatcccgccc ccgcagggcg
     9421 cggcggtccc cgcgctcggc ttcatgtccc agtgcggctt tcccgagacg gggcacctgc
     9481 gcttcgtgga gcggtatctc gagaagtata ccaagcgtct cggcgcgagc tacctgggct
     9541 ccatcatgaa gggcggcggc gaggggctcc acatgcagcc gaaatttctc gtggataagt
     9601 tcgaaaacgg cctgcattcc ctggg
//
LOCUS       DAORHF010000138         9593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6491_length_9593_cov_4.346299, whole genome shotgun sequence.
ACCESSION   DAORHF010000138 DAORHF010000000
VERSION     DAORHF010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6491_length_9593_cov_4.346299"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..111)
                     /locus_tag="PKJ16_11755"
     CDS             complement(<1..111)
                     /locus_tag="PKJ16_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012819668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNV47708.1"
                     /translation="MPDKKIKVLVVDDSALVRKIISDILQKDPAIEVVGTA"
     gene            complement(128..3424)
                     /locus_tag="PKJ16_11760"
     CDS             complement(128..3424)
                     /locus_tag="PKJ16_11760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV47709.1"
                     /translation="MAFSLGEYQDIFLEEADEQLQELNQNLLELESRPNDSEIINNIF
                     RAAHSLKSSAAFVGLNDLSDLAHKMENLLQGIRDGTMSVTRPVVQALFKSFDVINAVI
                     SAVSSGNEPKVPMGGVIAELQRISESETDAKPAAPEKSATPSAKPVEAPTRPAAPDED
                     VPKLVLSADDMRAIRQGVDAGMLGYDVTVFIDPAAVMKWMKAQLVLNNIEKIGTVVRS
                     IPDVEDLSEKQMPNVFKVIVLTDENLDAIRAACDVDLIYRVDLRDIAVSQVNGKTALT
                     FREEQTLISEKKEEQKKPVDVAAEAEEEEVVRPEEDEAEAEIEIGEEKDEDEAAHPEA
                     VQRAEGSKGAILKTVRVSIDKLDLLLNNVGELVIANSGFYRLYEDLRRRDMDKALVNE
                     FKNRMEQMSRIAKDLQSGIMKTRMVPIGQVLTRFKRLVRDLAMEFDKKVNLKILGEET
                     ELDKKVIDSIGEPLMHLLRNAIDHGIETASERVAMRKAEVATITLNAYQGGNQIFVEV
                     ADDGRGLDIDSIKRKAMEKGLATAEMIASMDNEDIFNFIFAPGFSTAKVITDISGRGV
                     GMNVVKEVVSELNGNVAIETEPGMGTRFVLSFPITLAIIPAIMVKVNREMYAIPLSDV
                     IETIKVAESDITTIEGHEVINLRGEILSLLRLNEFVALDSALGGNTRIPVVVVGYGNR
                     KVGLLVDYLEGKQEIVIKSLEQNYATVEGLAGASILGDGSICLILDIASMINKVINDQ
                     DFLMRRDRKKALDARIDEIKEVSAERIDLHGKTTAPKTDDGFSGGSREASAPAESARG
                     DFKDYEPFSISAQRVTDAKSAEKPVDAQRPVMAGIAKGDEPVADLLTRVAAEPPRTAV
                     DDDEDEEIKKRVQETLDGFRQDLKDTIKTAAESGTTDDHIMRSLGMTREDLSRVQVVS
                     NVGAANAAESLSKVLGKRIDLTIPEVRVLPIEKIPEFVGRIDNVFIGVYMPLTGDIRG
                     TVLFSFIEESGFELISLLYGKPVSDVKTIDEDGESALKEITNIVGSSVVNVFSEKINL
                     AIKPTVPTVVHDFMQSILDSMLVLHNISNDYALLMETDFYYEDDKVIGNLLILPDTES
                     LQVIVKKLRP"
     gene            complement(3441..4010)
                     /locus_tag="PKJ16_11765"
     CDS             complement(3441..4010)
                     /locus_tag="PKJ16_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV47710.1"
                     /translation="MGLEELLEKQKNFHGVPIEGSEAGVRSKIDAELMADSSVMQFVS
                     FLLEEVEFGINILSVHEILRMPEITRLPNTPDFIKGVINLRGNVIPIVDVRIRFDFPS
                     GQITDLTRVIVVETGEKLVGLLVDNVYQVVRLPEGNIDPPSDLIEGVSEEFITGIGRM
                     RDRLIVILNLDNILFSDEGRKAALGEQAK"
     gene            complement(4056..4373)
                     /locus_tag="PKJ16_11770"
     CDS             complement(4056..4373)
                     /locus_tag="PKJ16_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HNV47711.1"
                     /translation="MDNKIRVRIFGSYYNIQGEAPPEYILTLAEFVNDKMDEVSAGLP
                     GASAAQVAILAALNIADEYYQIRDSKTNVSDAIEKRARALISMLDEGLIGDVLNAAET
                     PRR"
     gene            complement(4400..5197)
                     /locus_tag="PKJ16_11775"
     CDS             complement(4400..5197)
                     /locus_tag="PKJ16_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HNV47712.1"
                     /translation="MISMQQLEELESRVIKALQLISDLRTENAKLEADNDVLKTQAED
                     AKLALEEKEKEVEEIKRELDAATRELANLKEKEAVLERKIIGLLGKLEGVQGGAAPAG
                     SPSTRAEAPMREEPPAKPAPRREEPRAAESPMKAGFAASSAPAASIPAASAPLKPEPA
                     KVEEEDEDIIIIDDDTDFEASGDVKLEKAADADDDEIILLEDDDDEIVIDDIDDDTII
                     IDDSEGSSKPPKASPSAAKKTEGKQPSKSTIVESDDDEFLIIEEDAK"
     gene            complement(5407..6357)
                     /locus_tag="PKJ16_11780"
     CDS             complement(5407..6357)
                     /locus_tag="PKJ16_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47713.1"
                     /translation="MKTPASISSAAPIRRCITSAVCAALVAFAGPVETARASLPLTET
                     INTLPEGSVELLLREEFIDARAGVQYRRDNAWLGLGIVPDLSIWIGFQYVHRHAPGSE
                     RSELGDSFLKIWYYLTDGFDDRFHIGLVGRFRFPTGRDAYAESDWHGAAFGNNELALG
                     PVMQVDVWRLHVHLNLLYVLREGGDEEFYSGLSLNPAEERTFRTVFGLDYTSPDSFLA
                     RERLKNDYFIFSAAVNTNAFYPVIPYAELYHARRLTRGDLGPDAIPIEGSLVNPVLVS
                     AGVRFFVLESLYLGLYGATSLGNERRHMRNAFGIEGSYQF"
     gene            complement(6374..6730)
                     /gene="rplT"
                     /locus_tag="PKJ16_11785"
     CDS             complement(6374..6730)
                     /gene="rplT"
                     /locus_tag="PKJ16_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011371085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HNV47714.1"
                     /translation="MPRATTGLVHRKRRSKVLKDAKGFRGARKNLYRTAKDARRRALQ
                     NSYIHRRAKKRDFRTLWIVRINAAARLCGISYSRLIAGMGAAGIQIDRKMLADIAVTD
                     MGAFKAIVGKVKGAAA"
     gene            complement(6767..6970)
                     /gene="rpmI"
                     /locus_tag="PKJ16_11790"
     CDS             complement(6767..6970)
                     /gene="rpmI"
                     /locus_tag="PKJ16_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005211352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HNV47715.1"
                     /translation="MPKMKTNRGAAKRFKITKNGKVKKANGFKSHLLEAKPSKRKRRL
                     RKATTASTSETEKIKRMMPYGRA"
     gene            complement(6999..7565)
                     /gene="infC"
                     /locus_tag="PKJ16_11795"
     CDS             complement(6999..7565)
                     /gene="infC"
                     /locus_tag="PKJ16_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HNV47716.1"
                     /translation="MSRFDTKDRNDSKRHRINDQIRAESVRLIGEGSEPVVIARDEAI
                     RIAREKDLDLVEISPDQSPPVVKIIDYSKFRFEQIKRAKEAKKRQKIIHIKEIKMRPS
                     IDSHDYAHKVKHARDFLADGDKVKFTIMFRGREIVHSQLGFKVMENVQNDLQDVAVIE
                     KKASQEGRNITMIMSPTPSSGSKKNKAE"
     gene            complement(7673..9583)
                     /gene="thrS"
                     /locus_tag="PKJ16_11800"
     CDS             complement(7673..9583)
                     /gene="thrS"
                     /locus_tag="PKJ16_11800"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018940435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HNV47717.1"
                     /translation="MTITLPDSTTLQAERGATVFDVIGAIGKGLQKAALAARVNGTPV
                     DLASALDADAALQVITFDGPEGRDVFWHSGSHLMAQAVKRLFPDARPTIGPAVDNGFY
                     YDFDTSANFTPEDLERIEAEMKKIVAEDLPVVREEMDRMKAIELFRAAGESYKVELLS
                     DLDAETVSVYRQGEFVDLCRGPHVPRTSYLKAFKLLTIAGAYWRGDERNKMLQRIYGI
                     AFPDEKQLKKHLQFLEEAKKRDHRRLGKELDLFSIHDETGAGLILWHPKGARLRNLIE
                     DFWRDQHYKNGYEMVVSPHIGKSQLWQTSGHLDFYNENMYSPMDIDGQNYYVKPMNCP
                     FHIVMYNNRGWSYRDLPLRWAELGTVYRYERSGVLHGLLRVRGFTQDDAHLYVRPDQM
                     PDEIDRVLAFSLYMLRSFGFQDFKVYLATRPSEKSVGEESMWRDAIAALEASVKRAGI
                     DYEVDEGGGAFYGPKIDIKIKDVLGREWQCSTIQFDFNLPERFDMTYIDTDGKKHRPY
                     MIHRALLGSLERFIGILIEHHGGRFPVWLAPVQAVIVTVNPEHADYAKGVEREMIARG
                     LRAECDLREETIGYKVREAITRHVPYVAVVGAKEVESGTLAVRRRGENSPETLAAGAF
                     MDRLAAESAERK"
BASE COUNT         1642 a         3030 c         2835 g         2086 t
ORIGIN      
        1 cgcggtgccc accacctcga tcgccggatc cttttggagg atgtcgctga tgattttccg
       61 cacgagcgcg gagtcgtcaa ccaccaggac cttgatcttt ttatcgggca tggccgctta
      121 tgacggttca gggacgaagt ttcttcacga tgacctggag cgattccgta tcgggaagga
      181 tgagcaggtt gccgatcacc ttgtcgtctt cgtaataaaa atcggtctcc atgagcagcg
      241 cgtaatcgtt ggagatgttg tgcagcacca gcatggagtc gaggatcgac tgcatgaaat
      301 catggaccac cgtcgggacc gtcggcttga tcgccaggtt gatcttctcg gagaacacgt
      361 tcaccaccga ggagcccacg atattcgtga tctccttgag cgcggattcg ccgtcctcgt
      421 cgatcgtctt cacgtcgctt accggcttgc catacaggag gctgatgagt tcgaaaccgg
      481 attcctcgat aaagctaaac aggaccgtgc cccggatgtc acccgtcagg ggcatgtata
      541 cgccgatgaa cacgttgtcg atgcgtccca cgaactcggg gatcttctcg atggggagaa
      601 cgcgcacctc gggaatggtg aggtcgatgc gtttccccag caccttggag agcgattccg
      661 ccgcgttggc ggcgccgaca ttggacacca cctgcacgcg cgacaggtct tcgcgcgtca
      721 tgcccagcga gcgcatgatg tgatcgtcgg tcgttcccga ctccgcggcc gtcttgatgg
      781 tatccttcag gtcttgccga aagccgtcga gcgtctcctg gacgcgtttc ttgatctcct
      841 cgtcctcgtc gtcgtcaacg gcggtgcgcg gcggctcggc ggccaccctg gtcaggagat
      901 cggcgacggg ctcgtcgccc ttcgcgattc cggccatcac cggtcgctgc gcgtccaccg
      961 gcttctcggc ggacttcgcg tccgtcaccc gctgggcgct tattgaaaac ggctcgtagt
     1021 ccttgaaatc accgcgcgcc gattcggctg gcgcggacgc ttcgcgcgat ccaccggaaa
     1081 aaccatcgtc cgttttaggc gcggttgtct ttccatggag atcgatgcgc tcggcggaaa
     1141 cctccttgat ctcgtcaatc cgcgcatcga gggctttttt gcggtcccga cgcatcagga
     1201 agtcctggtc attgatcacc ttgttgatca tcgaggcgat atccagaatc agacagatgc
     1261 tgccgtcccc gaggatcgag gcgcccgcga ggccctcgac cgtcgcgtag ttctgctcga
     1321 gggacttgat gacgatctcc tgcttgccct cgagataatc gacgagaagc cccaccttgc
     1381 ggttcccgta cccgaccacg accaccggga tgcgcgtatt tccgcccagc gccgagtcga
     1441 gcgcgacgaa ctcgttgagg cgcagaagcg acaggatctc gccgcggagg ttgatcacct
     1501 cgtggccctc gatggtggtg atgtccgact ccgcgacctt gatggtttcg atcacgtcgg
     1561 aaagcgggat cgcgtacatc tcgcgattca cctttaccat gatcgccggg atgatggcca
     1621 gggtgatggg gaaggagagc acgaacctcg ttcccatgcc gggctcggtc tcgatggcca
     1681 cgttcccgtt gagctcgctc acgacctcct tgaccacgtt catgccaacg ccgcgtcccg
     1741 agatgtcggt gatcaccttc gccgtcgaga acccgggggc gaaaataaaa ttgaagatgt
     1801 cctcgttgtc catgctggcg atcatctccg ccgtcgccag ccccttctcc atcgccttgc
     1861 gcttgatgct gtcgatgtcg agcccgcggc cgtcgtcggc cacctcgacg aatatctggt
     1921 tgccgccctg gtaggcgttc agggtgatgg tggccacctc ggcctttcgc atcgcgacac
     1981 gctcgctggc cgtctcgatg ccgtgatcga tggcattgcg cagcaggtgc atcagcggct
     2041 cgccgatcga gtcgatcacc tttttgtcga gctcggtctc ctcgcccagg atcttgaggt
     2101 tcaccttctt gtcaaactcc atggccaggt cgcgcaccag gcgcttgaag cgcgtgagca
     2161 cctgcccgat gggaaccatc cgcgttttca tgatgccgct ctgcagatcc ttcgcgatcc
     2221 gcgacatctg ttccatgcgg ttcttaaact cgttcaccag cgccttgtcc atgtcgcggc
     2281 ggcgcaggtc ctcatacaat cgatagaagc cggaattcgc gatcaccaac tcgccgacat
     2341 tgttgagcag caggtccagc ttgtcgatgg acacgcggac ggtcttgaga atggcgccct
     2401 tgctcccttc ggcccgttga acggcctcgg ggtgcgccgc ttcgtcctcg tctttctcct
     2461 cgccaatctc tatctcggcc tcggcctcat cctcctccgg acgaacaacc tcttcctcct
     2521 cggcctccgc agcgacatcc accggcttct tctgctcctc ctttttttcg gagatgagag
     2581 tttgctcctc gcggaaggtc aatgcggtct tgccattgac ctgcgagacg gcgatgtccc
     2641 gaaggtccac ccggtagatc aggtcgacgt cgcaggcggc gcggatcgcg tccaggttct
     2701 cgtcggtgag cacgatcacc ttgaacacgt tgggcatctg cttctcgctg aggtcctcga
     2761 cgtccggaat gctcctgacc accgtgccga tcttttcaat attattgagc acgagctgcg
     2821 ccttcatcca cttcatgacg gcggcgggat cgatgaacac ggtcacgtcg tagcccaaca
     2881 tcccggcgtc cacgccctgc ctgatggcgc gcatatcgtc ggcggaaaga acgagtttgg
     2941 ggacatcttc atcgggggcg gcgggacgcg tcggcgcctc cacgggtttt gcggaaggcg
     3001 tggcggattt ctcgggcgcc gcaggcttgg cgtccgtttc cgattcgctg atcctctgga
     3061 gctcggcaat gacgccaccc atgggtacct tgggctcgtt ccccgaagac accgcgctga
     3121 tgaccgcatt gatgacgtcg aaacttttaa acaacgcctg caccacgggg cgcgtgaccg
     3181 acatcgtgcc gtcgcggatc ccctgcagga ggttttccat tttatgggcg agatcggaga
     3241 gatcgttgag ccccacgaat gcggccgagc ttttcaggga atgggcggcg cggaaaatat
     3301 tgttgatgat ctcgctgtcg ttgggacggc tctcaagctc cagcaggttc tgattgagct
     3361 cctgcagctg ttcgtcggcc tcttcgagga agatgtcctg atattcgccc agtgaaaatg
     3421 ccatggtcgt gcacccgccg ttatttcgcc tgttcgccca gggcggcctt gcgcccctcg
     3481 tccgagaaca ggatattgtc gagattcaga atcacgatga gacggtcgcg catccgccca
     3541 atgccggtga taaactcctc ggacaccccc tcgatgagat ccgacggcgg gtcgatattg
     3601 ccctccggca gccgcaccac ctggtacaca ttgtccacca agagccccac gagcttctcg
     3661 cccgtctcca ccacgatcac gcgcgtgagg tcggtgatct ggccggacgg gaaatcgaac
     3721 cggatgcgga cgtcgacgat ggggatcacg ttcccgcgga ggttgatgac gcccttgatg
     3781 aaatccggcg tattgggaag ccgcgtgatc tcgggcatgc ggagtatctc atgcacggag
     3841 aggatgttga tgccgaactc gacctcctcc agaagaaagc tcacgaactg catcacgctg
     3901 gagtccgcca tcagctcggc gtctatcttg gatcgcaccc cggcctccga tccctcgatc
     3961 ggaacgccgt ggaagttctt ttgcttttcg aggagttctt ctaatcccat acccgctgtc
     4021 cttttcgcat catggccaac cgccgcggcg cgatgttacc ggcgcggcgt ctccgccgca
     4081 ttgagcacgt cgccgataag cccttcgtcg agcatcgaga tgagcgcgcg cgctcgtttc
     4141 tcgattgcgt cggacacgtt cgtcttcgag tccctgatct ggtaatactc atcggcgatg
     4201 ttcagcgccg ccaggatcgc cacctgcgcc gcgctcgccc cgggaagtcc cgcgctcacc
     4261 tcgtccatct tgtcgttcac gaattcggcc agcgtgagga tgtactccgg cggcgcctcg
     4321 ccctggatat tgtaatagct cccgaaaatt ctgaccctga tcttgttgtc cacggacatc
     4381 ccccgggagg ccgcaccgcc tacttcgcgt cctcttcgat gatgagaaac tcgtcatcgt
     4441 cgctttcgac gatcgtgctc ttgctcggct gcttgccctc ggtcttcttc gccgcggacg
     4501 gcgacgcctt cggcggcttg gacgaaccct ccgagtcgtc gatgatgatc gtgtcgtcgt
     4561 cgatatcgtc gatgacgatc tcgtcgtcat cgtcctcgag caggatgatc tcgtcgtcgt
     4621 cggcatccgc ggccttctcg agcttcacgt cgcccgacgc ctcgaagtcg gtatcgtcgt
     4681 caatgatgat gatgtcctcg tcctcctcct cgaccttcgc cggctcaggc ttcaagggag
     4741 cggacgcggc ggggatcgat gcggcggggg ccgacgatgc cgcaaaaccg gccttcatcg
     4801 gtgattccgc ggcgcgcggt tcctcgcgac ggggcgcggg ttttgcgggc ggctcttcgc
     4861 gcatcggggc ttcggcgcgg gtcgacggcg agccggccgg tgccgccccg ccctggacgc
     4921 cctcgagctt cccgagcaga ccgatgatct tgcgctcgag aacggcctcc ttctccttga
     4981 ggttggcgag ctcccgcgtg gcggcgtcga gctcgcgctt gatctcctcg acctcttttt
     5041 ccttctcctc gagcgccagc ttcgcgtcct cggcctgcgt cttcaatacg tcattgtccg
     5101 cttcaagctt ggcgttctcc gtacgcaggt cgctgatcag ctgtaacgcc tttatgaccc
     5161 tgctttcaag ttcttcaagc tgttgcatcg atatcatgat tgcatcctcc gcggtttcct
     5221 ggtctgctgt gcgacatcag ggacgggccc cgttcggctc cgccggccgc tgtaagcccc
     5281 tggtagaatt ttcggccaca ggcacggtaa acctgaattg aaccgtttat tgtaccttta
     5341 tacatgcggt gaattaaatc aagtattttt atttgaattt tccaagcgcg ccgcggccgc
     5401 tgcccgttaa aactgataac tgccctcaat gccaaacgcg ttgcgcatgt gtcgacgctc
     5461 atttccaagg ctggtcgcgc catagaggcc tagatacagg gattcaagga caaaaaaccg
     5521 gacgcctgcc gataccagga cgggattgac gagcgaaccc tcgatgggga tggcgtccgg
     5581 gccgaggtcg ccacgggtaa gccggcgggc atggtacagc tcggcatagg ggatcaccgg
     5641 ataaaaggcg ttggtgttga cggccgccga gaaaatgaaa taatcgtttt tgaggcgctc
     5701 gcgcgccagg aagctgtcgg gtgaggtgta atcgagcccg aagaccgtgc ggaaggtccg
     5761 ctcctcggcg gggttgaggg aaagcccgct gtagaactcc tcgtcgcccc cctcgcgaag
     5821 cacatacagg agattgagat gcacgtgcag ccgccagacg tcgacctgca tcaccgggcc
     5881 cagggcaagc tcattgttcc cgaaggcggc gccgtgccaa tcgctctcgg catatgcgtc
     5941 gcggcccgtc gggaaccgga atcggcccac aaggccaata tggaaccggt catcaaagcc
     6001 gtccgtgaga taataccaga tcttgaggaa ggaatcgccc agctcgctgc gttcggaacc
     6061 cggcgcatgg cgatgcacat actggaaccc gatccaaatg gaaaggtccg ggacgatccc
     6121 caggcccagc caggcattgt cccgacggta ctgaacgccc gcgcgcgcgt cgatgaactc
     6181 ctcgcgaagc aggagttcga ccgagccctc gggaagggtg ttgatggttt cggtgagcgg
     6241 aagggacgcg cgtgcggtct caacggggcc cgcaaacgcg acaagcgccg cacagacggc
     6301 gcttgttata catcgacgga ttggcgcggc gcttgatatg ctcgccgggg ttttcactcc
     6361 gcacccggcg aaattacgcg gccgccccct tcaccttgcc cacgatcgcc ttgaaggcgc
     6421 ccatgtcggt cacggcgatg tccgcgagca tcttgcggtc tatctggatc ccggcggccc
     6481 ccatcccggc aatcagccgg ctgtaggaga tgccgcacag gcgcgccgcc gcgttgatgc
     6541 ggacgatcca cagggtgcgg aaatcacgct tcttcgcgcg acggtgaata tacgagttct
     6601 gcagggcccg acgccgtgcg tccttcgccg tgcggtagag gttcttccgg gcgcccctga
     6661 atcccttcgc gtcctttaat accttgcttc ttcgtttccg gtgcacaagc ccggttgttg
     6721 ctcgtggcat aacactactc ctctctactg gtaaaagccg cgcgccctag gcgcgtccgt
     6781 acggcatcat tctcttgatc ttctcggttt ccgaggtcga ggcggtggtc gccttgcgca
     6841 ggcgccgttt gcgcttggag ggcttcgcct cgagcagatg gctcttgaag ccgttcgcct
     6901 tcttcacttt cccgttcttg gtgatcttga agcgcttcgc cgcgccccgg ttcgttttca
     6961 tcttcggcat cggtggttcc ccttttcgtg tgcgactgct actcggcttt attctttttc
     7021 gatcccgacg acggcgtcgg gctcatgatc atggtgatat tgcgcccctc ctgggacgcc
     7081 ttcttctcga ttacggcgac atcctgcagg tcgttctgga cgttctccat taccttgaat
     7141 cccagctggc tgtgaacgat ctcgcgcccg cgaaacatga tggtgaactt gaccttgtcc
     7201 ccatcggcga ggaaatcgcg cgcatgcttc accttgtgcg catagtcgtg gctgtcgatg
     7261 gacggccgca tcttgatttc cttgatgtga atgatcttct gtcgcttctt ggcctctttc
     7321 gctctcttga tctgctcgaa ccggaatttg ctatagtcga tgatcttgac gaccggcggg
     7381 ctctggtcgg gcgatatctc caccaggtcg aggtccttct cgcgtgcaat gcggatcgcc
     7441 tcgtcgcgtg cgataacaac gggctcgcta ccctcgccga tcaaccggac cgattcggcg
     7501 cgtatctggt cgttgatgcg gtgccgcttg ctatcgttcc tgtcttttgt gtcgaacctg
     7561 ctgatacatt acctcctcgc gcccatgatc atgccgtcat cggatcgcgc acatcgggcg
     7621 cgtaaaatga gcaagtagag aaacattcgc cgatattgtc aaagattttt ttctatttcc
     7681 gctcggcgga ctccgcggca agacggtcca tgaaggcgcc cgccgcgagc gtttcggggc
     7741 tgttctcgcc gcgacggcgc accgcgaggg tgccggactc gacctccttg gcgcccacca
     7801 ccgcgacgta cggaacgtgc cgcgtgatgg cctcgcgcac cttgtagccg atggtctcct
     7861 cgcgaagatc gcactcggcg cggaggccgc gggcgatcat ctcccgctcc acgcccttcg
     7921 cgtagtccgc gtgctcggga ttcaccgtca cgatgaccgc ctgcaccggg gccagccaca
     7981 cgggaaagcg cccgccatgg tgctcgatga ggatgccgat gaagcgctcg agcgagccca
     8041 ggagcgcgcg atggatcata tagggacggt gcttcttgcc gtcggtgtcg atgtaggtca
     8101 tgtcgaagcg ctcgggcaga ttgaaatcga actggatggt ggaacactgc cactcccgcc
     8161 ccagcacatc cttgatcttg atgtcgatct tggggccgta aaaggcgccg ccgccctcgt
     8221 cgacctcgta gtcgatgccc gcgcgcttca ccgacgcctc gagcgccgcg atcgcgtcgc
     8281 gccacatcga ctcctcgccc acggacttct ccgacgggcg cgtggcgaga tagaccttga
     8341 agtcctggaa cccgaacgag cgcagcatgt acaggctgaa ggcgagcacc cggtcgatct
     8401 cgtcgggcat ctggtccggg cgcacgtaaa ggtgcgcatc gtcctgcgtg aagccgcgca
     8461 cgcgcaacag gccatggagc acgccgctgc gctcgtagcg atacaccgtc cccagctccg
     8521 cccagcgcag cgggaggtcg cgatacgacc agccgcggtt gttgtacatc acgatatgga
     8581 agggacagtt catgggcttc acgtaatagt tctgcccgtc gatatccatg ggagaataca
     8641 tgttctcgtt gtagaagtcg agatgcccgc tcgtttgcca gagctgcgac ttgccgatat
     8701 gcggcgacac gaccatctcg tagccgttct tgtagtgctg atcgcgccag aaatcctcga
     8761 tgagatttcg caaccgggcg cccttggggt gccacaatat cagccccgcg ccggtctcgt
     8821 cgtgaatgga aaagagatcg agctccttgc ccagccggcg gtggtcgcgc ttcttggcct
     8881 cctcgagaaa ctgcaggtgc ttcttgagct gcttctcgtc ggggaaggcg atgccgtaga
     8941 tgcgctgcaa catcttgtta cgctcatcgc cccgccagta cgcgccggcg atggtgagca
     9001 gcttgaacgc cttgagatac gaggtgcgcg ggacgtgcgg tccgcggcag agatcgacga
     9061 actcgccctg gcggtacacg gaaactgttt cggcatcgag gtcgcttagc aattccactt
     9121 tgtatgattc gccggcggcg cgaaaaagct cgatggcctt catgcgatcc atctcctcgc
     9181 ggacgacggg caggtcctcc gcgacgatct tcttcatctc cgcctcgatg cgctcgaggt
     9241 cctcgggggt gaagttcgcg ctcgtatcga aatcgtaata gaagccgttg tccacggcgg
     9301 gcccgatggt ggggcgcgcg tcggggaaga ggcgcttcac cgcctgcgcc atgagatgcg
     9361 aacccgagtg ccaaaagacg tccctgccct cagggccgtc gaaggtgatc acctggagcg
     9421 cggcatcggc gtcgagcgcg ctcgcgaggt ccaccggcgt cccgttcacg cgcgccgcga
     9481 gggccgcctt ctgcaggccc tttccgatcg cgccgatgac atcgaacacg gtcgcgccgc
     9541 gctccgcctg gagtgtcgtg gaatcgggaa gggtgatcgt tatctggcct gcc
//
LOCUS       DAORHF010000139         9511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6584_length_9511_cov_4.917618, whole genome shotgun sequence.
ACCESSION   DAORHF010000139 DAORHF010000000
VERSION     DAORHF010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9511
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6584_length_9511_cov_4.917618"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="PKJ16_11805"
     CDS             <1..489
                     /locus_tag="PKJ16_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV47718.1"
                     /translation="IAINLRGVIDGCALFVPDLRRQGGGHIVNVSSCAGIATLPEMAS
                     YNATKAAVIALSETLRVELAPHGIGVTACCPTFIKTNLMERFTATEERQRAVTQEFFD
                     KSSYTAEAFAEHAIRSIEKNRMYAIPQMDGKLTWIIKRLFPETYYRVFAWEYARKMKR
                     GD"
     gene            complement(503..1246)
                     /locus_tag="PKJ16_11810"
     CDS             complement(503..1246)
                     /locus_tag="PKJ16_11810"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HNV47719.1"
                     /translation="MSVFFYTRGASPLPRLDPRAKITLLATVFAALSIAGHPVVMTAA
                     LIVLIALFALARSMASLRRMGPLFALIGAVTFGLWMLFYDGPLPPAHYAGAMSLRFMA
                     LLLAGLLFLSVTSLEEFTAGLIMMGLPYPVAFTVSLSFRLVIVFVATGHTIVEAQKVR
                     GNDVTRGSVPARIRGYVPLLIPLILNGIKKADTLTLALESKGFSPGNRIRIGDRYRLR
                     GADVIAIGAGVLVLAAVLLVRFGVIALPV"
     gene            complement(1251..2906)
                     /locus_tag="PKJ16_11815"
     CDS             complement(1251..2906)
                     /locus_tag="PKJ16_11815"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HNV47720.1"
                     /translation="MIDLRQAAFSYRSGRPPVIRNISLRIEPGEKVLVVGRNGAGKTT
                     LSKLLAGLIPHVERGEMSGDYLLDGTSVREHRYRDIVRRVAVLFQDFEAQIVSTGVLD
                     ELLFYPLNTGLDYATASERVRALAARFSLEHLLDREMGELSGGEKQKIALLALLAVNP
                     SVLILDEPFTDIEPATQEALLDFLNGYAGTVVLFEQVLDYHAYFDRIIVLCDGEVLRD
                     GDRDIVADAPLLDAAGLAMPALCRLGLSRRPVSVEECASIAKRRCSFDRKAHAAIIAR
                     DERSIAAAGEPLIEVRDLTFAYPGCARPVLDSVSLDIRRGDFVTVIGANGSGKTTLMK
                     LVAGILPFADGDILFRGKSIHDDPGFAGTVGYVYQNPDNQIFAETVFDEVGFILKMRG
                     VAGHELESRVSGILSRMGLADRRDADPFALPKGDREKVACASIIVGEPEVIILDEPTT
                     GLDYPSLAELMGIVSTLHRAGITILMITHSMETAAAYGSTILSMNEGRVLFYGNKREF
                     FSNDALIEAARIKRTDIMDLSMRLNGRMLMDAEEFRACWKRKR"
     gene            complement(2955..3515)
                     /locus_tag="PKJ16_11820"
     CDS             complement(2955..3515)
                     /locus_tag="PKJ16_11820"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HNV47721.1"
                     /translation="MRELFAVWTRTRQIVLIAVTAAIFAGTLIPFKPIQIVPGLTELR
                     PASVIPVLFGILFGPAAAWGSAIGNLIADFFGMLTPASAFGFVGNFLYSYVAFRVWRV
                     LVRGEITMNARQIGIFALCAVLASLVCALVVGLGVQLLGMAPFHVLFFIVFINNSVMA
                     GVLGPILMKLLYRRIDAMGLVYDGGR"
     gene            complement(3586..6882)
                     /locus_tag="PKJ16_11825"
     CDS             complement(3586..6882)
                     /locus_tag="PKJ16_11825"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HNV47722.1"
                     /translation="MAQALDGIGGGGFRLAAMCALLFLVMVGIVFGRSGRDAIFIREI
                     GSAKLPFMYLLTAVVMVGASGLYARVVDRMSRFRLLAVVLLAEVAVIALLRLLMILPH
                     SAVAVAVFCVTEVLLIIPPMHFWTYGNDVFNPREGKRAFPIIGAAGMLGTVAGGLLTR
                     AIAQNAGTPELFTAWALAGCLCLPAALAARPAKRDGNMRYAKDEKRDGPPAGRIGEVR
                     ELLSIPLVRTLALLHVPMYIVIYVIDYQFFSNLNEVFPDRDAMAGFLGVFTAVWSGSA
                     MAFQFLLAGPLLRRFGVGRVALVHPISVSLGAGALVARSLIALPVSGVGGFRSLLAVF
                     SKYSHISVKYGIDEPAYNLLFNSISRDRRGRSRALITGTVKPLCVALSGLFIIVMIDA
                     LGADIRLLSIATVALCAVWLVMSLRIQREYLQAMVANLESKNVELRDAAERQLTRSDI
                     AGSRDRLLRDVDSDDDAVAVYACRLLADIMDEGVARRLAARLPAVRDRVKRAILSSIA
                     GVRERDVVDAVMRAAGGSDPAVAAAALQALGRMGDPGVLPFVERFLDAGDPMLQGEAI
                     VACLMIDPVRDPESGPMRALRSLAQSDDPRLRLRAAASIRAAGGRDLVPILLRLSESG
                     DEAVRCEVIGALGETHDERAVPVLLEAAEDRRMRPYAFDALRRLGPIATSVVHAALDE
                     GGMPRARCVALASLLGHIGNPRSIPVLLGLLDDRPLPVQYAAIGSIAAIKAALMAQEG
                     VTEFIIMQLHFPADVRETVLERLSGFVVDVALDREHVAQLRALGGGPPALIVDALERW
                     SRRRIETCMQCLSIATDAMTVSTAARRLRSRDRRVRAEAVEVIEGLCAEARGLARIIE
                     SSWREEQPAHAAEAARLFRELMMKPDGDEWYRACVAFAAGRLRLGGLSGELESIAGGE
                     GARARVLAAWALRETGRPLAGGPDDKEMDAMNADMERILFLRSVPLFEEIEGGELRWI
                     SEIAAEETFFSGDIVFREGEEGDALYVIMHGSVKIVKGGQPPVTLCVLEERDYFGEMA
                     ILDEDVRSATVEVDRDARFLVIRREHFRTLLEARPQIAFAVFRTFSARLRDLQRQIVE
                     GHAASRVEIK"
     gene            complement(6909..8954)
                     /locus_tag="PKJ16_11830"
     CDS             complement(6909..8954)
                     /locus_tag="PKJ16_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV47723.1"
                     /translation="MRCRSLAVRYVMCAVIVGACAWWALPPIDAHADQEKRVLVLHSY
                     HKGLSWTEKVVSGIESEFAKAAAGGVRATIDYEYMDTKRFVDDAYFKILFDLYRHKSK
                     KLRYHVIISVDDAALVFLKRYRDRLYGEVPVVFCGVNFFTDDMLRGHKRYTGVVEAFD
                     VRATLDLALSLHPNTKEFVVVGDNTITSQSNVKALSAVAPVYLKKNVGMRFLTDADIW
                     RYARELERLGPGSVIIAMLFNLDNEGKFYTYEESFEIYTQYAKVPVYTYWDFYLGYGA
                     VGGKIISGVTQGEEAAKKAIQVLRGEKVQHIPILKESPNRYMFDYKVLRFYGIGESKL
                     PPNSIIINKPHELIDTFHRHKFLILGILAFILFLIGVIITLSVNIVRRKRVERELVRT
                     NAAYDRFVPHEFLNNLAKESIIDVQLGDQVQKDMTVLFSDIRSFTTLSEAMSPEENFN
                     FINSFLRAIGPIIRTHHGFIDKYIGDAIMALFPRDAEHAIMAAVEMQKGLIDWNASRR
                     ERGFLPVEIGIGLHIGTLMLGTVGEEKRMEGTVISDAVNLASRLEGLTKLFGAGIILS
                     ERTLEGLGEKKVKYLYRNLGRVSVKGKRDPVTIYEIIDGSDNAVAERKLKTKKLFEQG
                     LDLYYARDFAAAQGKFQNVLKLNPDDAAARLYERYAVELAQSPPPDDWDGVQRLDTK"
     gene            complement(8963..>9511)
                     /locus_tag="PKJ16_11835"
     CDS             complement(8963..>9511)
                     /locus_tag="PKJ16_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HNV47724.1"
                     /translation="DKRRIVHISDNSESSKYIHQELEAVDWSPLRLVSSSRCDTFAQW
                     KKAILEAHSKGDILLVTHYHTIKEKMTDKMVIPPKKIIDWTQPRLKMPAIGCWGFYVE
                     DGGMMAVAVSPYEQGEEAAKMAVEIIETKRRPKDIPVKTGVQYVVYIREKLVRDINMD
                     LPRMLEAFARATNNYFEEKIVQ"
BASE COUNT         1759 a         3197 c         3050 g         1505 t
ORIGIN      
        1 atcgccatca acctgcgggg cgtgatcgac ggctgcgccc tgttcgtccc ggacctcagg
       61 cgccagggcg gcggccacat cgtgaacgtc tcctcgtgcg cgggcatcgc cacgctgccc
      121 gagatggcca gctacaatgc gaccaaggcc gccgtgatcg cgctctcgga gacgctgcga
      181 gtggagcttg cgccccacgg catcggcgtc acggcgtgct gccccacgtt catcaaaacg
      241 aacctcatgg agcgcttcac cgcgacggag gagcgccagc gcgccgtcac gcaggagttc
      301 ttcgataaat ccagctatac cgcggaggcc ttcgcggagc acgccatccg ctccatcgag
      361 aagaaccgca tgtacgccat cccgcagatg gacggtaagc tcacttggat catcaaacgg
      421 ctgtttccgg aaacatatta ccgcgtgttc gcatgggagt atgcgcggaa gatgaagcgc
      481 ggggactaac cgcgcaggcg gatcacaccg gcagggcaat gacgccgaac cgcacaagca
      541 acaccgccgc gagcacaagc acgcccgcgc cgatcgcgat cacgtcggcg ccccgcaggc
      601 gatagcgatc gccgatcctg atccggttgc ccggcgagaa acccttcgac tcgagcgcga
      661 gcgtgagcgt atcggccttc ttgatgccgt tgaggatgag cgggatgagg agcggcacgt
      721 accccctgat gcgcgcgggg acgctccctc gcgtcacgtc gtttccccgc accttctgcg
      781 cctccacgat ggtgtgccct gtggccacaa acacgatcac gaggcgaaac gagagcgaca
      841 ccgtgaaggc caccggatac ggcaggccca tcatgatgag ccccgcggtg aactcctcca
      901 gcgaggtcac cgacaggaag agcagtcccg ccaggagcag ggccatgaac cggaggctca
      961 tggcccccgc atagtgcgcc ggcgggagcg gcccgtcgta gaagagcatc cacagaccga
     1021 acgtgacggc gccgatgagc gcgaagagcg gtcccatccg cctgaggctt gccatgctcc
     1081 gcgcgagcgc gaaaagcgca atcaacacga tgagcgctgc cgtcatgacg acgggatggc
     1141 cggcgatcga gagcgcggcg aagacggtcg ccagtagcgt gattttcgca cgcggatcga
     1201 gccgagggag ggggctcgcg ccccgggtgt agaagaacac gctcatggct tcaccgcttc
     1261 cgcttccagc atgcgcggaa ctcctccgcg tccatcagca tccgtccgtt gaggcgcatc
     1321 gagaggtcca tgatgtccgt ccgcttgatc cgcgcggcct cgatcagcgc atcgttcgag
     1381 aaaaactcgc gcttgttccc gtagaagagc accctccctt cgttcatcga caggatggtc
     1441 gatccgtagg cggcggccgt ttccatggaa tgggttatca tcaggatggt gatcccggcc
     1501 cggtgcaggg tgctcacgat gcccatgagc tcggccagcg agggataatc cagcccggtg
     1561 gtcggctcgt cgaggatgat gacctcgggc tcgccgacga tgatcgaggc gcaggcgacc
     1621 ttttcgcggt cccccttggg gagggcgaag ggatcggcgt cgcggcggtc ggcgagcccc
     1681 atccgcgaca ggatgccgct cacgcgcgat tcgagctcat gccccgccac ccccctcatc
     1741 ttcaggataa agccgacctc gtcgaagacc gtctccgcaa atatctggtt gtcggggttc
     1801 tggtagacat agccgacggt gccggcgaag ccgggatcat catggatgct cttcccccga
     1861 aagaggatat cgccgtcggc gaacggaagg atgccggcga caagcttcat cagcgtggtt
     1921 ttgccggacc cgttcgcgcc tatgaccgtc acgaaatcac cgcgacgtat atcgagggac
     1981 accgagtcga gcaccggccg cgcgcatccc ggatatgcga aggtgagatc gcgcacctcg
     2041 atgaggggct cacccgcagc ggcaatcgac cgttcgtcgc gggcgatgat cgcggcgtgc
     2101 gcctttcggt cgaacgaaca ccgtcgcttc gcaatcgacg cgcactcctc gaccgaaacg
     2161 ggccggcggg aaagcccgag ccggcagagg gcgggcatcg caagtccggc cgcatcgagc
     2221 aggggcgcat cggcgacgat gtcgcgatca ccatcgcgga gcacctcccc gtcgcagagc
     2281 acgatgatgc gatcgaagta tgcatgatag tccagcacct gctcgaagag caccaccgtc
     2341 cccgcatagc cgttcaggaa gtccaggagg gcctcctgcg tcgccggctc gatgtcggtg
     2401 aagggctcgt cgaggatgag cacggacgga ttgaccgcga ggagcgccag gagcgcgatc
     2461 ttctgtttct ctccgccgga gagctcgccc atctcccgat cgagcagatg ctccagggag
     2521 aaccgcgccg caagggcgcg cacccgctcg ctcgccgtcg catagtcgag gccggtattg
     2581 agcggataga agagaagctc gtcgagaacg ccggtggaga cgatctgcgc ctcgaaatcc
     2641 tgaaagagca ccgcgaccct cctgacgatg tcccgatagc ggtgctctcg cacgctcgtg
     2701 ccgtcgagga gatagtcgcc gctcatctcc ccgcgctcga catgcgggat gaggccggcg
     2761 agcagcttcg agagggtggt cttcccggcg ccgttcctgc cgaccaccag caccttctcg
     2821 cccggctcga tccgcagcga gatatttcgg ataacgggcg gacgaccgct ccggtagctg
     2881 aatgcggcct gtcgaagatc gatcatggtc gatagcgggc gccgcccgcc gtgctgcggg
     2941 acggcctcgc ggccctagcg gccgccatcg tacacaagcc ccatcgcgtc gatccgccgg
     3001 tagaggagtt tcatgaggat ggggccgagg accccggcca tcaccgaatt gttgataaag
     3061 actatgaaaa agagcacatg gaacggggcc atgccgagga gctgcacgcc gagcccgacg
     3121 acgagcgcgc agacgaggct tgcgaggacg gcgcacagcg cgaagatccc gatctggcgc
     3181 gcgttcatcg tgatctcgcc gcgcaccagg acgcgccaga cccggaaggc gacataggaa
     3241 tagaggaagt tcccgacgaa cccgaaggcg ctggcgggcg tgagcatgcc gaagaaatcg
     3301 gcgatgaggt ttccgatggc cgatccccac gccgcggcgg gaccgaagag gatcccgaag
     3361 aggacgggaa tgacgctcgc gggccgcagc tccgtaagtc cgggaacgat ctggatgggc
     3421 ttgaacggga tgagcgtccc ggcgaagatg gcggcggtga ccgcgatgag cacgatctgg
     3481 cgcgtccggg tccacacggc gaacagttcc ctcattggct ccctcctccc ggcgcacggc
     3541 ggccggcacg ggacccctcc taccagcggg cgcgcgcggt gtaaactatt ttatttccac
     3601 ccgcgatgcg gcgtgtccct cgacgatctg ccgctgcagg tcccgcaggc gcgcgctgaa
     3661 ggtgcggaac accgcgaatg cgatctgcgg ccgcgcctcg agcagggtgc ggaagtgctc
     3721 gcgacgtatc accaggaagc gggcgtcgcg gtccacctcg acggtcgccg agcggacgtc
     3781 ctcgtcgagg atcgccattt ccccgaagta atcgcgctcc tccaggacgc agagcgtgac
     3841 cggcggctgt ccgcccttga cgatcttcac gctcccgtgc atgatcacgt agagcgcgtc
     3901 gccctcctcg ccctcccgga agacgatgtc ccccgagaag aaggtctcct ccgccgcgat
     3961 ctcgctgatc cagcgcagtt cgcccccctc gatctcctcg aagagcggca cgctcctgag
     4021 aaacaggatc cgttccatgt cggcgttcat cgcatccatc tccttgtcgt cgggcccgcc
     4081 cgcgagcgga cgccctgttt cacgcaacgc ccaagcggcg aggacgcgcg cgcgggcgcc
     4141 ctcccccccg gcgatcgatt cgagctcccc ggagagcccc cccagccgca gccgcccggc
     4201 cgcgaaggcg acgcaggcgc ggtaccactc gtccccgtcc ggtttcatca taagctcccg
     4261 gaagagccgc gccgcctccg cggcatgggc gggctgctcc tcgcgccagg aggactcgat
     4321 gatccgcgcg agcccgcgcg cctcggcgca caggccctcg atcacctcga ccgcctcagc
     4381 gcgcacccgc cggtcgcggc tccggagacg gcgcgccgcc gtgctcaccg tcatcgcgtc
     4441 ggtcgcgatc gacaggcact gcatgcaggt ttcgatccgc ctgcggctcc agcgctccag
     4501 ggcgtccacg atgagcgccg gcggtccgcc accgagggcc cgcagctgcg ccacgtgctc
     4561 gcgatcgagc gcgacatcca ccacgaaccc cgatagccgc tccaggaccg tttcgcggac
     4621 atcggccgga aaatgaagct gcatgatgat gaattccgtg acgccctcct gggccatgag
     4681 ggcggccttg atcgcggcga tggagccaat ggccgcgtac tggacgggaa gcggacgatc
     4741 gtcgagcagg cccagcagca cggggatgga acgcggattc ccgatgtggc ccaggagcga
     4801 ggccagcgcg acacagcgcg cacggggcat gccgccctca tcaagcgccg cgtgcaccac
     4861 cgacgtcgcg atgggaccga gacgcctgag cgcgtcgaat gcgtagggcc gcatgcggcg
     4921 gtcctccgcg gcctcaagca gcaccggcac cgcccgctcg tcatgcgttt cacccaacgc
     4981 gccgatcacc tcgcagcgca ccgcctcgtc cccggattcc gaaagccgga ggagaatcgg
     5041 caccaggtcg cgcccgcccg cggcgcggat gctggcggcg gcgcgcagcc gcaggcgcgg
     5101 atcgtccgac tgcgcaaggg agcgcaatgc ccgcatggga ccgctctcgg gatcgcgcac
     5161 cggatcgatc atgaggcagg cgacgatcgc ctcgccctgc agcatcggat cgccggcatc
     5221 aagaaaccgc tcgacaaacg gcaggacgcc cgggtccccc atgcggccga gcgcctggag
     5281 ggccgcggcg gcgacggccg gatcggaacc gcccgctgcg cgcatcaccg catcgaccac
     5341 atcgcgttcg cggacgccgg cgatcgacga caggatcgcg cgcttcacgc gatcgcgcac
     5401 agcgggaagc ctcgcagcca ggcgccgcgc gaccccctcg tccatgatgt ccgcgagcag
     5461 gcggcaggcg tacaccgcca cggcgtcatc gtccgagtcc acgtcgcgca aaagccggtc
     5521 gcggcttccc gcgatgtccg accgcgtgag ctggcgctcg gcggcgtcgc gcagttcgac
     5581 gttcttcgat tcgaggttgg cgaccatcgc ctgcaggtac tcgcgctgga tgcgcaggct
     5641 catgaccagc cacacggcgc acagcgcaac cgtcgcgatc gagagcagcc ggatatcggc
     5701 gcccagcgca tcgatcatca cgatgatgaa cagccccgag agggccacgc acagcggctt
     5761 caccgtgccg gtgatgagcg cgcggctcct gccgcgccgg tcgcgcgaga tagaattaaa
     5821 gagcaaattg taggcgggct cgtcgatgcc gtacttcacc gagatgtggc tgtacttcga
     5881 gaagacggcc aacagcgacc ggaaaccgcc cacaccggac accggaagcg ctatcaggct
     5941 ccgggccacg agcgcgcccg cccccaggga cacgcttatg ggatggacga gcgcgacccg
     6001 ccccacgccg aaccgacgca ggagcggccc ggcgagcagg aactggaacg ccatggcgct
     6061 cccggaccac accgcggtga acacgcccag aaaaccggcc atggcgtcgc ggtccggaaa
     6121 gacctcgttg aggttggaga agaactggta gtcgataaca tagatgacga tgtacatcgg
     6181 cacgtgcagc agcgcaagcg tccgcaccag cgggatcgag agcagctcgc gcacctcgcc
     6241 gatgcggccg gcgggcggcc cgtcgcgctt ttcatctttc gcataacgca tatttccgtc
     6301 gcgcttcgcc ggccgggcag ccagcgccgc cggcaggcac aggcagccgg cgagcgccca
     6361 tgccgtgaag agctccggcg tcccggcgtt ctgcgcaatc gcgcgcgtca gcaggccgcc
     6421 ggcgaccgtg cccagcatcc ccgccgcccc gatgatcgga aaggcccgct tcccctcccg
     6481 cggattgaac acgtcgttcc cataggtcca gaagtgcatg ggggggatga tgaggagcac
     6541 ctccgtcacg cagaagaccg cgacggcgac ggccgaatgc ggaaggatca tgaggaggcg
     6601 caggagcgcg atcacggcga cctcggcgag cagcacaacc gcgagaaggc gaaagcggga
     6661 catgcggtcc acgacgcgcg catacaagcc cgacgcgccc accatgacga cggccgtgag
     6721 gaggtacatg aacggaagct tcgccgaacc gatctcgcgg ataaagatcg cgtcacggcc
     6781 gctgcgcccg aaaacgatcc ccaccatgac gagaaaaagg agcgcgcaca tggccgccag
     6841 ccggaacccg ccgccgccga ttccgtccaa cgcctgtgcc acgcgtgcgc gcacggggtg
     6901 cctgagggct acttggtgtc caggcgctgc acgccgtccc agtcgtccgg gggcggtgat
     6961 tgcgcgagct cgacggcata gcgctcgtac aggcgcgcgg ccgcgtcgtc cggattgagc
     7021 ttcaggacgt tctggaactt gccctgcgcc gccgcgaaat cgcgcgcgta gtagaggtcg
     7081 aggccctgct cgaacagctt cttggtcttg agcttgcgct cggcaacggc gttatcgctc
     7141 ccgtcgatga tctcatagat ggtcacgggg tccctcttcc ccttgacgct cacccggccg
     7201 aggttccggt agagatactt caccttcttc tcgcccagcc cctcgagggt ccgctcgctc
     7261 aggatgatgc cggcgccaaa gagcttggtg agcccctcga gccgcgaggc gaggttcacc
     7321 gcgtcggaga tgacggtgcc ctccatacgc ttctcttcgc ccaccgtccc cagcatgagc
     7381 gtcccgatat gcaagccgat cccgatctcg acggggagga agccgcgctc acgccgggag
     7441 gcgttccaat cgatgagccc cttctgcatc tcgacggccg ccatgatcgc gtgctccgcg
     7501 tcgcgcggaa acagggccat gatggcgtcg ccgatatact tgtcgatgaa gccgtggtgc
     7561 gtgcggatga tggggccgat cgcgcgcagg aacgagttga tgaaattgaa gttctcctcc
     7621 ggcgacatgg cctcggaaag cgtggtgaag ctgcggatgt ccgagaagag gaccgtcatg
     7681 tccttctgca cctggtcccc gagctgcacg tcgatgatgc tctccttggc caggttgttc
     7741 aggaactcgt gcgggacaaa gcggtcgtag gccgcattgg tgcgcaccag ctcccgctcc
     7801 acgcgcttgc gccgcacgat gttcacgctc agcgtgatga tcacgccgat gaggaagagg
     7861 atgaacgcga ggatccccag gatcaggaac ttgtgccggt gaaaggtgtc gatgagctcg
     7921 tggggcttgt tgatgatgat actgttgggc ggcagcttcg actcgccgat cccgtaaaag
     7981 cgcagcacct tgtaatcgaa catgtagcgg ttggggctct ccttgaggat gggaatgtgc
     8041 tgcaccttct ccccgcggag gacctggatg gccttcttcg cggcctcctc gccctgcgtc
     8101 acgccgctga tgatcttgcc gccgacggcg ccgtacccca gatagaagtc ccagtaggtg
     8161 tataccggga ccttcgcata ctgcgtatag atctcgaagc tctcctcgta ggtgtagaac
     8221 ttcccctcgt tgtcgagatt gaagagcatg gcgatgatca cgctcccggg gccgagccgc
     8281 tcgagctcgc gcgcatagcg ccagatgtcg gcgtcggtca ggaagcgcat gcccacgttc
     8341 tttttcaggt ataccggcgc gacggcgctc aacgccttca cattgctttg cgaggtgatg
     8401 gtgttgtcgc ccaccaccac gaactccttt gtattgggat gaagggacag cgccagatcg
     8461 agcgtggcgc gcacgtcgaa ggcctcgacc acgcccgtgt atcgcttgtg ccccctgagc
     8521 atgtcgtcgg tgaaaaagtt cacgccgcag aagaccaccg gcacctcgcc gtagagccgg
     8581 tcccggtagc gcttgaggaa caccagcgcc gcatcgtcga cggagatgat cacgtgatag
     8641 cggagctttt tgcttttatg ccggtagaga tcgaagagga tcttgaagta ggcgtcgtcc
     8701 acgaaccgct tggtgtccat gtactcgtag tcgatggtcg cccgcacccc gcccgccgcg
     8761 gccttcgcga actccgactc gatcccggag accaccttct cggtccagct caggcccttg
     8821 tggtaggagt ggagcacgag cacgcgcttc tcctggtcgg cgtgcgcgtc gatgggcggg
     8881 agcgcccacc acgcgcaggc gccgacgatc acggcgcaca tcacataacg cacggcaagg
     8941 ctgcggcacc gcataccggc tcctattgga cgattttttc ctcgaagtaa ttgttggtcg
     9001 cgcgagcgaa ggcctccagc atgcgcggga ggtccatgtt gatgtcacgg accagcttct
     9061 ctcgaatgta gacgacatac tgcacgcccg tcttcaccgg gatgtccttc gggcgccgct
     9121 tcgtctcgat gatctcgacc gccatcttgg ccgcctcctc gccctgttcg tagggcgaca
     9181 ccgcgaccgc catcatcccg ccgtcctcga cataaaagcc ccagcaaccg atggccggca
     9241 tcttcaagcg gggttgcgtc caatcgatga tcttcttcgg cgggatcacc atcttgtccg
     9301 tcatcttttc ctttatggtg tgatagtgcg tcaccagcag aatatcgccc ttggaatggg
     9361 cctcgaggat cgccttcttc cactgcgcga aggtgtcgca gcgcgacgac gagacgagac
     9421 ggagcggcga ccagtcgacc gcctcgagct cctggtgaat gtacttcgac gactcggagt
     9481 tgtccgaaat gtgcacgatg cgccgcttgt c
//
LOCUS       DAORHF010000140         9489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6604_length_9489_cov_6.321285, whole genome shotgun sequence.
ACCESSION   DAORHF010000140 DAORHF010000000
VERSION     DAORHF010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6604_length_9489_cov_6.321285"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1771)
                     /locus_tag="PKJ16_11840"
     CDS             complement(<1..1771)
                     /locus_tag="PKJ16_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV47725.1"
                     /translation="MSRFNLSEWALEHTQFVVFFMLLAVAMGILSYKRLGRSEDPSFT
                     IRCMIISSYWPGASARMVEDQVTDKIEKKLQETRGLDYLQSFTRAGVSVIYVYLKDEV
                     LAADIRPTWTQVRNSVGDIRATLPRGVVGPFFNDRFDDTFGSIYALTADGFTTAELKE
                     YADKVRLKIMTVPDVAKADFLGVQSEVIFVEIENSRLATLGLEPDTVIKALQAQSIIA
                     PAGMVDTATDNIYLRVSGMFQSVEQIRGTGIEANNRILRLGDIARIRRAYSEPSDPKM
                     YFNGQPAIGLAISMTDGGNVLDLGENLNALMAQLKKELPAGIDIHQVSDQPKVVEESI
                     GEFMEALIEAVIIVLAVSFVSLGVRAGIVVALAIPLVLCTVFTCMELLGIDLHKISLG
                     ALIIALGLLVDDAIISVEMMALKLEQGWDRVKAAGYAFTATSFPMLTGTLITAAGFIP
                     VGFSKGGASEYAGAIFSVVGIALLVSWVVAVTVTPLLGHAILKEDRSAAIGGTEHEIA
                     DTKFTTVFRATLVWCLRNRRTVVGATLATFAAAVAINPLVPKEFFPASPRPELIVDMR
                     LPEGSSLSATDREVRKFNELIRKD"
     gene            complement(1768..2853)
                     /locus_tag="PKJ16_11845"
     CDS             complement(1768..2853)
                     /locus_tag="PKJ16_11845"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV47726.1"
                     /translation="MRRSRIIIPLVTVACLALPGCGKKESDGSAVPVVRTIKVSRAPF
                     SDNSSYSGVVCGRYESKLSFQVGGRIIKRFVEVGTAVRAGQALMQLDQSDLRDQMEAS
                     GSQVEAARADLKLAEDNLRRYKELFDNDAVSKAEFERYQNAHAVAAAKLASLKKNYDV
                     GANQFRYGRLVAPANGVITEIAAEAGQVIAPGQSVMTLVRHGEREVQVSIPENRINEI
                     RSARRITITFWANPDLSVRGRVREISPVSDRATRTYTVRVSLIAPPPAVKLGMTATVS
                     VSFGAARPAVFVPVSAIFQTGDRATVWAVSSERARRRVVTLGPYGDNEVEVRDGLADG
                     DVVVTAGVHKLVDGQRVRVMESSEMFK"
     gene            3035..4492
                     /gene="pcnB"
                     /locus_tag="PKJ16_11850"
     CDS             3035..4492
                     /gene="pcnB"
                     /locus_tag="PKJ16_11850"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HNV47727.1"
                     /translation="MAFLSFFKKKKYPIVLKRDSHSISPGMVDPGALFVMRRLRQSGF
                     EAYLVGGCVRDILLGRNPKDFDVTTDARPNQVKRLFRKCFLIGRRFRLAHVYTGTDEF
                     IEVATFRALVDAEEEGISKVAANNVFGTIEEDALRRDFTINALYLNSADLSVVDFTGG
                     LRDIKKKLLRSIGDPVQRFSDDPVRMVRLARFCAMLGFSPSREDHRAARLLAHHIKDA
                     NANRMLDEMYKIFKCGASAGTFARLWEFGVLQHWIPELAPERYRDPLMKRLAVIDERR
                     ARGEEIPGNVIIAALFYDVFSGAEGASGSLQDALHAIHKRHHELAVRLRLPRVEWEKV
                     CNTVARQTVFTRPEGGKKWSQFRAKFVRNAYFPDALTFFEIMVKATGRDESELRAWWR
                     QTPEGERSAHGDIRSRSDRTPSRRRGRGPRDGEPRTDHTAPAEDSAIAHNVDATPIDG
                     QAADSGTGAPKKKRRRRGGKRRRGGPRPEAQPASL"
     gene            4679..5980
                     /locus_tag="PKJ16_11855"
     CDS             4679..5980
                     /locus_tag="PKJ16_11855"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HNV47728.1"
                     /translation="MARKATCGLIGFGTVGSGVYALLEKNAALIEQRTGVAVSITAIC
                     DLRTDEVRKRTAGVAVTGDWRQVVDDPEIDTVIELIGGLDPAKAIVLAALERGKNVVT
                     ANKKLLAEDGGEIFARARTKPAGLGFEAAVGGGIPCILSLRSGLVGNRVSTVMGILNG
                     TTNYILTQMTDGGLSFDAALAIAQQKGFAEADPTFDVEGFDAGHKIAILSMLAFGRSV
                     DYRAVPMEGITKIRDIDIAYAREMGYVIKLLGIGKLVGGEIDIRVHPTMLPARHPLAA
                     VRNEFNAVLLEGDMTGPVVLYGRGAGGAPTASAIVSDIIAIASNGPGAAAAPAPAGGA
                     SVIGPEKRLSRYYLRLTTQDRPGILAQIAGALGRCGISIASVIQREGSAEYVPLVIMT
                     HAAGEDRLQEALGEINTYPFVRDAAMMIRVEDADALGESHE"
     gene            5973..7316
                     /gene="thrC"
                     /locus_tag="PKJ16_11860"
     CDS             5973..7316
                     /gene="thrC"
                     /locus_tag="PKJ16_11860"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HNV47729.1"
                     /translation="MSRKRFSAVFRCINGCEEHPLDEIVYRCSVCGSLLEVRHDIDKL
                     RKKKPAEWKALFDGRVMTTKWPYGSGVWGKKEWVCPEVDNENVVSMFEGNTNLFWARR
                     FGKMLGMDDLWVKMCGNSHTGSFKDLGMTVLVSMVNEMIARGKKIQAVACASTGDTSA
                     ALAAYCAYAGIPSIVFLPANKISTAQLVQPMANNAIVMSLDTDFDGCMRVVQEITKDN
                     SIYLANSMNSLRVEGQKSISIELLQQFNWQVPDTIVIPGGNLGNVSALGNGFTMLYDL
                     RVIRKKPRIVLAQAEKANPLYRSYRTGFEQWESITPEKTLASAIQIGDPVSRDKAIRT
                     LKEFDGVVEQASEEELANAAALADRTGMYSCPHTGVALAALIKLVEKKVISKKERVIV
                     ISTAHGLKFSEFKVGYHEGTLAGVSSTYANKPIMLPPDAGRVREALDRELAARAK"
     gene            7342..8001
                     /locus_tag="PKJ16_11865"
     CDS             7342..8001
                     /locus_tag="PKJ16_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV47730.1"
                     /translation="MQITTNVYQVGGSDESYGQDAAIYLVRDGDRAALVDAGTGYGHT
                     AVLDNVRNAGIEPSNIELLFLTHCHYDHTGGASLMRQATGCKIVAHTLDAVYLEAGDA
                     EVTAASWYGSFFRALPVDVTVSEAKREFSVGSLSVMMHHIPGHSPGSTALTVVSDGKL
                     VLFGQDVHGPLNDTLLSNRDQYEKSLEFLISLDADILCEGHFGVYEGKERVRRFIESY
                     L"
     gene            8128..>9489
                     /locus_tag="PKJ16_11870"
     CDS             8128..>9489
                     /locus_tag="PKJ16_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47731.1"
                     /translation="MNTKAEAVEYTPFAWNVKVKFLLTELVGAIVGAWLAVIYVAFFI
                     GLTAEQGMIALRHSWWIVLSATALALPTNEILFHPIHKFLKCFKKKTVDYKILTAAYI
                     RANNIPLRHGFFMWTRFMVGAVSTTIVYKVFIPEPKTVYQLINTVTVVCYCGFVSGVI
                     AYLTSERVFARFLADLNRSVKHIPRKLILDKRIHRVSIRRRMIIILLPLMFLTILVIG
                     MNSYQEVNTVIKTGGNASLQADYLAGFLLRLFFVLASSTLFTFVAVYLSASNTVRPLS
                     SAVMHLREISKGNLGEKLVIDTQDEIRDVLYEIMLTVMNLERIISRFSSSIGAFHDLS
                     RVLDVISESIGEGVKIQRDAIEKSVERVRSLADSAGSVRASVEGASASVAEIFAAVEQ
                     FVASLQSSGKLVQDVRTEGEQLSERLKDGEARLAGMVGDMEKIQRSSERIMDITTVIA
                     EIAD"
BASE COUNT         1735 a         2989 c         3079 g         1686 t
ORIGIN      
        1 ggtctttccg gatgagctcg ttgaatttac ggacctcgcg gtcggtggcc gacagggacg
       61 atccctcggg gagccgcatg tcaacgatga gctccgggcg gggcgaggcc gggaagaact
      121 ccttgggcac cagggggttg atcgcgaccg cggcggcgaa ggtcgcgagc gtcgcgccca
      181 ccacggtcct ccggttgcgg aggcaccaga cgagcgttgc gcgaaatacc gttgtgaatt
      241 tcgtgtccgc gatctcgtgc tccgtcccgc cgatcgcggc ggagcggtcc tccttgagaa
      301 tggcgtgacc cagaagcggc gtgaccgtta ccgcgaccac ccaggagaca aggagcgcga
      361 tccccaccac cgagaagatc gccccggcat actccgatgc gccccctttg gagaagccca
      421 cggggataaa ccccgccgcg gtgataagcg tgccggtgag catgggaaac gaggtcgccg
      481 tgaaggcgta cccggccgcc ttcacgcggt cccagccctg ttccagcttg agcgccatca
      541 tctcgaccga tatgatggcg tcgtccacga gcagccccag ggcgatgatg agcgcgccca
      601 gcgaaatctt gtgcaggtcg atccccagga gctccatgca ggtgaacacg gtgcacagca
      661 ccagcggaat ggccagcgcc accacgatcc ccgcgcgcac gcccaggctc acgaagctca
      721 ccgcgagcac gatgatcacc gcctcgatga gcgcctccat gaattcgccg atcgattcct
      781 cgaccacctt cggttgatcg gagacctggt gtatgtcgat ccccgccggg agctccttct
      841 tgagctgggc catgagcgca ttgaggttct cccccagatc gagcacgttg ccgccgtcgg
      901 tcatggagat ggccaggccg atcgccggct ggccgttgaa gtacatcttc gggtcgctcg
      961 gctcggagta ggcgcgtcgg atccgcgcga tgtcgcccag gcggaggatg cggttgttcg
     1021 cctcgatgcc ggtcccgcgg atctgctcca cgctctggaa catgccgctc acgcgcaggt
     1081 agatattgtc ggtggcggta tcgaccatcc ccgccggcgc aatgatgctc tgcgcctgca
     1141 gcgccttgat tacggtgtcc ggctccaatc caagcgtggc cagacggctg ttctcgatct
     1201 cgacgaagat cacctccgat tgtacgccga ggaaatcggc cttggcgacg tcgggcacgg
     1261 tcatgatttt taggcgcacc ttgtccgcgt attccttgag ctcggcggtg gtgaagccgt
     1321 cggcggtgag ggcgtagatg gagccgaagg tgtcgtcgaa gcggtcgttg aaaaacggcc
     1381 caacgacgcc ccgcggcagg gtggcgcgga tgtccccgac cgagttgcgc acctgcgtcc
     1441 aggtcggccg gatgtccgcg gccaggacct cgtctttcag gtacacgtag atgacggaga
     1501 cgccggcccg cgtgaagctc tgcaggtagt ccaggccgcg cgtctcctgc agcttcttct
     1561 cgatcttgtc ggttacctgg tcctcgacca tgcgggcgct cgcgccgggc caataactcg
     1621 agatgatcat gcagcgaatc gtgaacgagg ggtcctcgga gcggcccagg cgtttgtagg
     1681 aaaggatgcc catcgccacc gcgaggagca tgaagaagac gacgaactgc gtgtgctcca
     1741 gcgcccactc gctgaggttg aagcggctca tttgaacatc tcagacgatt ccatgacgcg
     1801 cacccgctgg ccgtccacga gcttgtgcac gccggcggtc accaccacgt ccccgtcggc
     1861 gaggccgtcg cgcacctcga cctcgttgtc cccgtagggg cccagggtga cgacgcggcg
     1921 ccgtgcgcgt tcgctggaaa ccgcccacac cgtcgcgcga tcgcccgtct gaaaaatcgc
     1981 ggaaacggga acgaacaccg cggggcgggc cgcgccgaag gaaacggaga ccgtcgcggt
     2041 catccccagc ttcacggcgg ggggcggggc gatgagcgaa acgcggaccg tgtaggtgcg
     2101 cgtggcgcgg tcggatacgg gcgatatctc gcggacccgt ccgcgaaccg aaaggtcggg
     2161 attcgcccaa aaggttatgg tgatgcggcg agccgaccgg atttcgttta tgcggttttc
     2221 ggggatgctc acctggacct cgcgctcgcc gtggcgcacc agcgtcatca cggattggcc
     2281 cggcgcgatc acctggcccg cctcggcggc gatctcggtg atcaccccgt tcgccggcgc
     2341 gacgaggcgt ccgtagcgga actggtttgc gcccacatcg taattttttt tcagcgaagc
     2401 gagcttggcc gccgcgacgg catgggcgtt ttgatagcgc tcgaactccg ccttcgagac
     2461 ggcgtcattg tcgaaaagct ccttatagcg gcggagattg tcctcggcga gtttcaggtc
     2521 ggcgcgcgcc gcctcgacct gcgagcccga cgcctccatc tggtcgcgaa ggtcgctctg
     2581 gtcgagctgc atgagcgcct gcccggcgcg gaccgcggtg cccacctcga cgaagcgctt
     2641 gatgatgcgc ccccccacct gaaacgagag tttgctttcg tagcggccgc acaccacgcc
     2701 ggagtagctg gaattgtcgg aaaagggggc gcgggacacc ttgatggtgc gcaccacggg
     2761 gacggcgctg ccgtccgatt cctttttccc gcagccgggg agggcgaggc acgccaccgt
     2821 cacgagagga atgatgattc gtgaacgccg catgacaacg accttagggg ggcaaaaggt
     2881 aaaatcaagc gaaattttcc tcatactgtt catcgtggac cgggcgacag aaaagtcttg
     2941 accgatatgc ttctttactt ataggtgtcc accgaagagc agaaaagatg tattgaacag
     3001 aaaacccccg gctcttgcgt gaaaataaac ggttatggcg ttcttgtcct tttttaaaaa
     3061 aaagaagtat cccatcgttt taaagcgtga cagccattcc atctcgcccg gcatggtcga
     3121 tcccggcgcg ctgttcgtca tgcggcgcct gcgccagagc ggattcgagg cctatctcgt
     3181 gggcggttgc gtgcgcgaca tcctgctggg ccgcaatccg aaggacttcg acgtgaccac
     3241 cgacgcgcgt cccaaccagg tgaaacgtct ttttcggaag tgttttctca tcggccgtcg
     3301 ctttcgtctc gcccacgtat atacggggac cgacgagttc atcgaggtgg ccaccttccg
     3361 cgcgctggtc gacgcggagg aggagggtat cagcaaggtc gcggccaaca acgtcttcgg
     3421 gaccatcgag gaggacgcgc tccgccgtga tttcaccatt aacgcgctgt atctcaatag
     3481 cgccgatctc tccgtggtcg atttcacggg cggtctgcgc gacatcaaga aaaagctcct
     3541 gcgctcgatc ggggacccgg tccagcgctt cagtgatgat ccggtccgca tggtgcgcct
     3601 ggcgcgcttc tgcgcgatgc tcgggttttc gccgtcgcgc gaggaccatc gcgccgcgcg
     3661 gctgctcgcg caccacatca aggacgccaa cgccaaccgc atgctcgacg agatgtacaa
     3721 aatcttcaaa tgcggcgcgt cggccggaac cttcgcgcgc ctctgggaat tcggcgtgct
     3781 gcagcactgg atcccggagc ttgcgccgga acggtatcgc gatccgctca tgaagcgcct
     3841 cgcggtcatc gacgaacggc gcgcgcgggg cgaggagatc cccgggaacg tgatcatcgc
     3901 cgcgctcttt tacgacgtct tctccggggc cgagggggcg tccggttcgc tgcaggacgc
     3961 gttgcacgcg atccacaagc gccatcacga gctcgcggtg cgcctgcggc ttccgcgcgt
     4021 ggaatgggaa aaggtctgca atacggtggc gcgacagacc gtgttcaccc gtcccgaggg
     4081 cgggaagaag tggagccagt ttcgggccaa gttcgtccgc aacgcgtatt ttcccgacgc
     4141 gctcaccttt ttcgagatca tggtgaaggc gaccggcagg gacgaatcgg agctgcgcgc
     4201 gtggtggcgg caaacgccgg agggcgagcg aagcgcccac ggcgacatcc gcagccgttc
     4261 agaccgaacg ccgtcgcgac gtcgcggccg cggaccgcgg gacggcgagc cccggaccga
     4321 ccacacagcg ccggcagaag acagcgctat tgcgcataac gtcgatgcga cgcccatcga
     4381 cgggcaggcc gcggattccg gcaccggtgc gccgaagaaa aagcgccgtc gccgcggcgg
     4441 gaaacggcgt cgcggcggtc cccgccccga ggcgcaaccg gcttcactct aggccccact
     4501 cccatcatcc ccctgccgta acgtgttcga tgaacgcagg tgtgatcgcg cgcgcgtgcg
     4561 cgccgcggcg tcacgcctcc gccggtatta gggttgacat ttcccgcatt ggttttttgc
     4621 tattctcgta tgccgcgcgg cgatgcccgc gggcacccgg ctggcggagg gacgatagat
     4681 ggcacggaag gcaacctgcg ggctcatagg attcgggacg gtcggaagcg gcgtctacgc
     4741 gctcctcgag aagaacgccg cgctcatcga acagcggacc ggcgtcgcgg tgtcgatcac
     4801 agccatctgc gatctgcgca ctgacgaggt gaggaagcgg accgccggcg tcgcggtgac
     4861 gggagactgg cggcaggtgg tcgacgatcc ggagatcgat acggtcatcg agctcatcgg
     4921 ggggctcgat ccggccaagg cgatcgtcct ggcggcgctc gagcgcggca agaacgtcgt
     4981 gaccgcgaac aagaagctcc ttgcggagga cggcggggag atcttcgcgc gggcgcggac
     5041 gaaaccggcg gggctcggct tcgaggccgc ggtgggcggc ggcatcccct gcatcctctc
     5101 gctccggtcc ggcctcgtgg gcaatcgcgt gtcgaccgtg atgggcatcc tcaacgggac
     5161 caccaactat atcctcacgc agatgaccga cggggggctt tccttcgacg cggcgctcgc
     5221 gatcgcgcag cagaagggct ttgccgaggc cgacccgacc ttcgacgtgg aggggttcga
     5281 cgccgggcac aagatcgcca tcctctccat gctcgccttc ggccgctcgg tggactaccg
     5341 cgccgtgccg atggagggca tcaccaagat ccgcgatatc gatatcgcct acgcgcgcga
     5401 gatgggatac gtcatcaagc ttctggggat cggcaagctg gtgggcggcg agatcgacat
     5461 ccgcgtgcac ccgaccatgc ttccggcgcg ccacccgctc gccgcggtgc gaaacgagtt
     5521 caacgcggtg ttgcttgagg gcgacatgac cgggccggtg gtcctctacg gccgcggtgc
     5581 cgggggggcg cccaccgcct cggcgatcgt aagcgatatt attgcaattg cgtctaacgg
     5641 acccggggcc gcggccgcgc cggcccccgc gggcggggcg agcgtcattg gccccgagaa
     5701 gcgcctctcg cgctactacc tgcgcctcac cacccaggac cggcccggca tcctcgcaca
     5761 gatcgccggc gcgctggggc gctgcgggat ctccatcgcc tcggtgatcc agcgcgaggg
     5821 cagcgccgag tacgtgccgc tggtgatcat gacgcacgcc gcgggtgagg accgcctgca
     5881 ggaggcgctg ggcgagatca acacgtatcc gttcgtccgc gatgcggcga tgatgatccg
     5941 cgtcgaggac gccgacgccc ttggagaaag tcatgagtag aaaacggttt tccgcggtct
     6001 tccgctgcat caacgggtgc gaggagcacc ccctcgacga gatcgtgtac cgctgctccg
     6061 tctgcgggag cctcctcgag gtgcgtcacg atatcgacaa gctgcgaaaa aagaaacccg
     6121 ccgagtggaa ggcgctcttc gacggccggg tgatgacgac gaaatggccc tacgggagcg
     6181 gcgtgtgggg caagaaggaa tgggtgtgcc ccgaggtgga caacgagaac gtcgtgtcga
     6241 tgttcgaggg gaacaccaac ctgttctggg ccaggcgctt cgggaagatg ctgggcatgg
     6301 acgatctctg ggtgaagatg tgcggcaaca gccacaccgg ctcgttcaag gacctgggga
     6361 tgacggtcct cgtctccatg gtgaacgaga tgatcgcccg cgggaaaaag atacaggcgg
     6421 tggcctgcgc gtccaccggc gacacctcgg cggcgcttgc ggcctactgc gcctacgcgg
     6481 ggatcccgtc catcgtgttc ctgccggcca acaagatctc caccgcgcag ctcgtgcagc
     6541 cgatggcgaa taacgccatc gtgatgtcgc tggacacgga cttcgacggc tgcatgcgcg
     6601 tggtgcagga gatcacgaag gacaacagca tctatcttgc gaactcgatg aactcgctgc
     6661 gcgtggaggg acagaagtcc atatcgatcg agctgttgca gcaattcaac tggcaggtgc
     6721 ccgacaccat cgtgatcccc ggcgggaacc tcggcaacgt ctcggcgctc gggaacggct
     6781 tcacaatgct ctacgacctc agggtgatcc gcaagaagcc gcgcatcgtg ctcgcgcagg
     6841 ccgagaaggc caatccgctc taccggtcgt atcggaccgg cttcgagcag tgggaatcca
     6901 ttaccccgga gaagacgctc gcctccgcga tccagatcgg cgatccggtc tcgcgcgaca
     6961 aggcgatccg aaccctcaag gagttcgacg gcgtcgtgga acaggcgagc gaggaggagc
     7021 tcgccaacgc cgcggcgctc gcggatcgca cgggcatgta ctcctgtccg cacaccggcg
     7081 tggcgctcgc cgcgcttatc aagctcgtcg aaaagaaggt gatatcgaaa aaggagcgcg
     7141 tcatcgtcat ttccaccgcg cacggcctca agttctccga gttcaaggtg ggctaccacg
     7201 aggggacgct cgcgggcgtt tcgagcacat atgcgaacaa gccgatcatg ctcccgccgg
     7261 acgcggggag ggtccgcgag gcgctggacc gcgagctcgc cgcgcgcgcg aagtaagggg
     7321 gcgcggtccg ggaggacgac gatgcagatc accacgaacg tataccaggt cggcggatcg
     7381 gacgaatcgt acgggcagga cgccgcgatt tatctcgttc gcgacgggga ccgcgccgcg
     7441 ctcgtcgacg ccgggaccgg atacggccac acggccgttc ttgacaatgt gcgcaacgcg
     7501 gggatcgagc catcgaacat cgagcttttg tttctcaccc attgccacta cgatcacacc
     7561 ggcggcgcgt cgctcatgcg gcaggcgacc ggctgcaaaa tcgtcgccca tacgctggac
     7621 gccgtttatc tcgaagcggg cgatgccgag gtgaccgccg cctcgtggta cggctcgttc
     7681 ttccgggcgc ttcccgtgga cgtcacggtc tccgaggcga agcgggagtt ttccgtggga
     7741 tcgctttcgg tgatgatgca ccatattccc gggcactcgc cgggatcgac cgcgctcacc
     7801 gtggtctccg acggaaagct cgtgctcttc ggacaggacg tccatgggcc gctgaacgac
     7861 acgctcctgt cgaaccgcga tcaatacgag aaatcgctcg aattcctcat ctcgctggat
     7921 gccgacatcc tctgcgaggg gcattttggc gtctacgagg ggaaggagcg cgtgcggagg
     7981 ttcatcgagt cgtacctgtg aggacgacgg gtggcggatg gtctattcgg cgatatccgg
     8041 atgcgtcggc caatttatgg ttgaaatttt catatacaat taatattctg caaacacaga
     8101 gataacatcc gatcgtccca gaggacaatg aatactaaag cggaagcggt cgagtacacg
     8161 cccttcgcgt ggaacgtgaa ggtcaagttc ctgctcaccg agctcgtcgg cgcgatcgtg
     8221 ggcgcctggc ttgcggtcat ctacgtggcc ttctttatcg ggctcaccgc ggagcagggc
     8281 atgatcgccc tccggcactc ctggtggatc gtcctctcgg cgaccgcgct tgcccttccc
     8341 accaacgaga tactcttcca tcccatacac aaatttctca aatgcttcaa gaaaaagacg
     8401 gtcgattaca agatcctcac cgccgcgtat atccgcgcga acaatatccc gctgcggcac
     8461 ggctttttca tgtggacgcg ctttatggtg ggcgcggtgt cgacgacgat agtatacaag
     8521 gtgttcatcc cggagcccaa gaccgtctac cagctcatca acaccgtcac ggtggtctgt
     8581 tattgcggat ttgtttcggg ggtgatcgcc tatctgactt cggagcgggt ctttgcgcgt
     8641 ttcctggccg atcttaaccg ctcggtcaag catatcccgc gcaagctcat cctggacaag
     8701 cggatccacc gggtgtcgat ccgccggcgg atgatcatca ttcttctacc gctcatgttc
     8761 ctcacgatcc tggtcatcgg catgaactcc taccaggagg tgaacaccgt catcaagacc
     8821 ggcggcaatg cctccctgca ggcggactat ctcgccgggt ttctgctgcg gctctttttc
     8881 gtcctcgcct cgtcgaccct gttcaccttc gtcgcggtgt acctctccgc gtccaatacg
     8941 gtgcgcccct tgagctcggc cgtcatgcac ctgcgggaaa tatccaaggg caacctcggg
     9001 gaaaagctcg tcatcgacac ccaggacgag atccgcgacg tcctctacga gatcatgctc
     9061 acggtgatga acctggagcg catcatctcg cgcttctcca gctcgatcgg ggcgtttcac
     9121 gacctctccc gcgtgctgga tgtgatctcg gagagcattg gcgagggggt caagatccag
     9181 cgcgacgcga tcgagaaatc cgtggagcgg gtccgctcgc tggccgattc cgcggggtcc
     9241 gtgcgcgcga gcgtcgaggg cgcgtcggcg tcggtcgcgg agatcttcgc cgcggtggag
     9301 cagttcgtcg cctcgctcca gagctcgggg aagctcgtgc aggacgtgcg caccgagggc
     9361 gagcaactct cggagcggct caaggacggc gaggcccggc ttgccggcat ggtgggcgac
     9421 atggaaaaga tacagcggag ctcggagcgg atcatggaca tcaccaccgt gatcgccgag
     9481 atcgcggac
//
LOCUS       DAORHF010000141         9466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6645_length_9466_cov_5.158007, whole genome shotgun sequence.
ACCESSION   DAORHF010000141 DAORHF010000000
VERSION     DAORHF010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9466
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6645_length_9466_cov_5.158007"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="PKJ16_11875"
     CDS             <1..195
                     /locus_tag="PKJ16_11875"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HNV47732.1"
                     /translation="IVTGRKIGDYVNELRVRDAAAMLDGTSENIIDIAFAVGFESLRT
                     FNRAFLKVMNVTPTEYRERT"
     gene            complement(202..1170)
                     /locus_tag="PKJ16_11880"
     CDS             complement(202..1170)
                     /locus_tag="PKJ16_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HNV47733.1"
                     /translation="MNTYHKASFYLTAGFPVWVLAASITALIHPPVFTWFSGPLITAG
                     LGIIMLGMGLTLTVDDFRRIARFPGRVLLGVLLQYTVMPLSGWAIARLFGLPDVFAVG
                     LILVASCPGGTASNVLAYLARADVPLSVTMTSVSTLLAAFATPFWTWFLAGSRVDVPA
                     LGLFLTTVQVILLPVVAGILMNRYLPRATVAILPAAPVAAVLFITLIVASIIGAGRDE
                     ILHAGAPLVAAVCSLHASGFCFGYALSWLAARDVTVSRTISIEVGMQNSGLGVVLARA
                     NFANPSVAIPCAISSLMHSLIASVLAAWWRRTAHNATDDTETDPAQ"
     gene            complement(1402..3273)
                     /locus_tag="PKJ16_11885"
     CDS             complement(1402..3273)
                     /locus_tag="PKJ16_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47734.1"
                     /translation="MYISIKKRMLIAGIIIIAIVTVFGSTVIFMFNLTQKSYDDVNRL
                     SENTILLKSRLIEHISWSENLLLSAVTGNAFAGQTDPHFCAFGKWYYGLKQQGKAAPM
                     SEEQRALFDKLEEPHKKLHATAELITKSGSTATAVTIYRTTTQAHLKVLREIFDGYIK
                     NNEATQQRISRATSIRNATITAIIVISNVLVTGLSILVVTLFNKKFSRSFSRMENGIY
                     RLSHGDLAFEIETKKIDCSTDRDCRKNDCPCFGKETNSCFLEVGSYAPMLKNEVQCPA
                     ILNRTYPDCRQCPVMNRLVSDEFEFIIILLDQFRLRLRRVVSSVKEIVFSLSSSTEQM
                     SSALGSFSTNLQSEAANIEEIMATVEEVFSGIEGIAVSAEQQNTGVKELTETIAKHSG
                     IIESLASLSHESHTFSERISTQAIKGDESLKQMERSIETISSSSGEMTAIVKIINDIS
                     DQINLLSLNAAIEAARAGDAGRGFAVVADEISKLADQTAQSIKSIDGLIKLNETETTR
                     GVDITRDTVAVFSEIIDSVEGIKRMLKSITENTGEQLEINRSIGSMAQRSKSMSEEIT
                     YATEEQKNAFNEILKAVSSINELTQTNASSSEEIASGSEEIAANADTVNREIDFFTV"
     gene            3638..4468
                     /locus_tag="PKJ16_11890"
     CDS             3638..4468
                     /locus_tag="PKJ16_11890"
                     /inference="COORDINATES: protein motif:HMM:NF017808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HNV47735.1"
                     /translation="MKKILIITMAIFMMATVAASAKTYDLVLVHGLTNKHQWSDAFLD
                     KVASIWGSGNVYVIYTNESTRVWTRSINGRTIYFCGENDFSAGDDTIYDQATLMRTKI
                     TKLNSSYGLSAKFNVIAHSMGGLVSRCYIKRFPNTVAGLVTLGTPHQGSPLANVAAWL
                     SFFIGAEAAANQLKPAYVKNTFNPAYPVVGAPLADSGKIYTIGGDGDGWDCWGWGGEL
                     QLGWDTLTTVYWKDSDGAVARGDEQISGATHVATFWSYDHMELVTKADVATKAAAYLR
                     "
     gene            complement(4551..5084)
                     /locus_tag="PKJ16_11895"
     CDS             complement(4551..5084)
                     /locus_tag="PKJ16_11895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47736.1"
                     /translation="MKFLAWSLLLAIVIITPTFANTGPVVQKYRDGGGWKEPYPGPDR
                     AEIALIAGSYRVLISGNRTMWHDKILTIKSITVVPVKDIYPTLTMAASITVPRYWDTV
                     KELPIEAYRLQQRFDDEKKRTRITGLSFTIDVNLKESGRERFEFEGYYIERKRSFAGT
                     VKHNGDIVGGFIAEKIR"
     gene            complement(5390..6181)
                     /locus_tag="PKJ16_11900"
     CDS             complement(5390..6181)
                     /locus_tag="PKJ16_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV47737.1"
                     /translation="MNSPDISVFANLKDRALRDEGIFIAEGGFLVERALASGLEMIAT
                     LCSARMAERIAPLAQGRCPLVIADESEIARLVGFPFHRGVMTAGRRPAPLGIERFLAS
                     NPPARRLVICAGLDSDDNLGSIMRSAAALDYDAIARTPGGSDPWSRRALRASMGAGFT
                     LPVLAIGREDDAVGALRDHGFAVYATVLASDARPIPAFRACGKHALVFGNEASGLDAA
                     WRDRCDRAITIPIARGDSLNVGVAAGIFMYHCSLLAGDGDPAEHP"
     gene            6632..7531
                     /locus_tag="PKJ16_11905"
     CDS             6632..7531
                     /locus_tag="PKJ16_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47738.1"
                     /translation="MKKSIAVVAIVVMAVCATAAFAGGSTPTCNTKYPIVLAHGMGAS
                     AQILGIIDYWNDIPGALRDKGAKVYITSVNGMDSTANKAASWKSQFLNIKAVTGAAKL
                     NVIGHSHGTIYTRYAISNLGLSSYVASHTSIAGPHRGSAVADVVLGVIPNPCQALVGA
                     TLDFVYAFVFGDKNPSSLQNGKDLVRSYMINTFNPNTPNKSGIYYQSWAGKIKTMAID
                     ARNWYFLITWPILKYYEGDNDGLVSVTSAKWGTFRGVIEGAWYSAGVDHLNMVDQPFG
                     ITPGFDAPEQFVSLVSELKSKGY"
     gene            7853..>9466
                     /locus_tag="PKJ16_11910"
     CDS             7853..>9466
                     /locus_tag="PKJ16_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HNV47739.1"
                     /translation="MISFIALSGCGSKGGDGGGGGGGGAGSGASNGTYTEVNTTAKTG
                     TIIGAVSGQDYRVAGGVQSPNNDSYALGGATITITHESLIEGGTLVVNVPSSGDYQIV
                     LPAGTGYTIVFAMPGYESVTYTDVEIIADQTITLETAVLKNNALTVVVDGTVSNNGTG
                     LSGVQVTIQCSGDNCDGIADVTTYTENDGTFYVNLPAGHCYTVTYDLAGYQSANGGEF
                     CITDNGTVTGFNGTVTLNPIVTTGTLAGIITGETLAGGTAGIKATIQISKDGAVIRTY
                     ETDDDGNYSITLDGGSGYTATVVALGYTTITTGSFSIAAGETKSIEGASLRLVTGTLI
                     GSVTDASGAAMGGVSISIYYLDGTVVRASTSTNDNGSFSIVLPVISSQSGEGDYYRVV
                     FQKPGYVDTVYHVYDIFQSLEVSLQPVVVVIDGYGIVSGSIISSVDGRGVSGAVIKLR
                     SGIDVKTGVYMSVSGATVTATSDANGAYRIQGLPAGSYTGEVVKSGYTTGYFTIVCVK
                     DVETPDQRCILSPTDSVSPTSGRIEVVLKW"
BASE COUNT         2024 a         2597 c         2792 g         2053 t
ORIGIN      
        1 atcgtcaccg ggaggaagat cggcgactat gtcaacgagc tgcgcgtccg ggacgcggcc
       61 gcgatgctcg acggcaccag cgagaacatc atcgacatcg cctttgccgt gggctttgaa
      121 agcctgcgca ccttcaaccg cgccttcctc aaggtgatga acgtgacccc caccgaatac
      181 cgcgagcgca catagcgcgc gtcactgcgc cgggtcggtc tccgtatcgt ccgtcgcgtt
      241 gtgcgccgtc cgacgccacc aggcggcgag gacgctcgcg atgagcgagt gcatgaggct
      301 ggagatggcg cagggaatgg ccacggaggg gttcgcgaaa ttcgcgcgcg ccagcaccac
      361 gcccagtccg gagttttgca ttccaacctc gatggagatg gtgcgcgaga ccgtaacgtc
      421 gcgcgccgcc agccatgaca gcgcgtaccc gaagcagaaa cccgaggcgt gcagcgaaca
      481 cacggccgcg acgagcggcg ccccggcgtg gaggatctcg tcgcgccccg caccgatgat
      541 agaggcgacg atgagcgtga tgaacagcac ggccgcaacc ggcgccgccg gcagtatcgc
      601 gacggtcgcg cgcgggagat agcggttcat gagaatgccg gcgaccaccg gcagcaggat
      661 cacctgcacc gtggtcagga agagccccag ggccggcacg tccacccggc ttcccgcgag
      721 aaaccaggtc caaaacggcg tcgcaaacgc cgcgagcagc gttgaaacgc tggtcatggt
      781 gaccgacaac ggcacgtcgg cgcgcgcaag ataggcgagc acgttcgacg cggtgccgcc
      841 ggggcaggag gccaccagga tgagccccac ggcgaacacg tccgggagac cgaagaggcg
      901 cgcgatggcc cacccggaaa gcggcatcac cgtatattgt aacagtacgc caagaaggac
      961 gcgacccgga aagcgcgcga tccgtcgaaa gtcgtcgacg gtcagcgtaa gccccatccc
     1021 caacatgatg atgcccaggc ccgccgtgat gagcggcccc gagaaccagg tgaacaccgg
     1081 aggatgaatg agcgcggtaa tgctggccgc aagaacccag accgggaatc cggcggtgag
     1141 ataaaatgac gccttatggt atgtattcat tgatattagc gcctggtcga aattgccata
     1201 cattcaactt tcagtttcga tagatagtca agaatgaaat tatcatagct ctgacccttt
     1261 gagggttgag cgttcaatct acgattgctt gccgtgagat gggttacctc ccgattcctg
     1321 tttgcgagga ccctgagggt gctccttggg agatggaggc gttggagaat caacgaggat
     1381 gtttagcgta cggttgttac gttatactgt gaaaaaatca atctctcgat tcacggtgtc
     1441 ggcgtttgcc gctatttcct ccgagccgct ggcaatttcc tcggatgacg atgcgttcgt
     1501 ctgggtgagt tcgttgatgc tgctgacggc ctttaagatc tcattgaacg cgtttttttg
     1561 ctcttccgtc gcgtaggtta tctcttcgga catgctcttg cttcgttgcg ccatcgaacc
     1621 gatggacctg ttgatttcga gctgttcccc ggtattctcc gttatcgatt tcagcatgcg
     1681 cttgattcct tcaacgctat cgatgatctc gctgaatact gcgaccgtgt cccgtgtgat
     1741 gtcgaccccg cgggtcgttt cggtctcgtt gagtttaatg agaccgtcga tgcttttaat
     1801 gctctgggcg gtctggtcgg ccagcttgga aatctcgtcg gctacaaccg caaatccccg
     1861 tcccgcatcc ccggcacggg ccgcctcgat tgccgcattg agggacaaga gattgatctg
     1921 atcggagatg tcattgatga tcttcacgat ggcggtcatc tcccctgagc tgctgctgat
     1981 ggtttcaatg ctgcgctcca tctgcttgag ggactcgtcg ccctttatcg cctgtgttga
     2041 tattcgttcc gaaaaggtgt gcgattcgtg tgataatgat gcaagggact cgattatgcc
     2101 gctatgtttg gcgatggtct ccgtaagctc tttcacgccg gtgttctgct gctcggcgct
     2161 aacggcgatt ccttcgatac cggagaagac ttcttcgacg gttgccatta tctcctcaat
     2221 gttggccgcc tcggattgca aattggtcga aaacgatcca agggccgatg acatttgttc
     2281 ggtggatgag gaaagtgaga atactatttc cttgaccgag gagacgaccc tgcgaaggcg
     2341 aagccgaaac tggtcgagaa ggatgatgat gaattcgaat tcatcactga cgagccggtt
     2401 catgacgggg cactgacggc aatctgggta tgtacggttc agaatcgccg ggcactggac
     2461 ttcgttcttc agcatcggcg cataggagcc gacctcgagg aagcaggagt tggtttcttt
     2521 tccgaaacac gggcagtcat ttttgcggca atcgcggtca gtgctgcagt ctatcttctt
     2581 cgtttcgatt tcgaatgcaa ggtcgccgtg tgaaagccgg tagatgccgt tctccatgcg
     2641 ggaaaaagag cgggagaatt ttttattgaa gagcgtgacg acgagtatcg agagcccggt
     2701 gaccagtacg ttcgaaataa cgataatggc ggtgattgtc gcgtttcgaa tcgaggtggc
     2761 ccgggatatt cgctgctggg tcgcttcgtt gttctttata tacccgtcga agatttcgcg
     2821 caacaccttc aagtgggcct gtgtcgtcgt tcgatagatg gttactgcgg tagcggtcga
     2881 accgctcttt gtgatcaatt ctgctgttgc gtggagtttt ttatgcggct cttcaagttt
     2941 gtcgaataaa gcgcgctgct cctcggacat tggtgcagcc ttcccctgtt gtttcagccc
     3001 ataataccat tttccgaagg cacaaaagtg cggatctgtc tgcccggcaa atgcgttccc
     3061 ggttacggct gagaggagga ggttttccga ccaggagata tgttcgatga ggcggctttt
     3121 cagcaggatt gtgttctcgc tgaggcgatt aacatcatcg taagattttt gcgtcaggtt
     3181 gaacatgaaa atgacggttg atccaaagac ggtcacgatc gcgatgatga ttattcctgc
     3241 gatcagcatt ctctttttaa ttgagatata cacgactgcc tcctggacgg aattcgcaaa
     3301 gggtataggc tacctcatta ttcattcaat tcaaaaaaat attcgatact gaagctgttc
     3361 tatacatgag tggagaatcc cttcgttcgg cgtgctactt tattgttgag cccaaatata
     3421 agtaatcggg ctctgacccc atgctgcgat gtcccaaaat ctctcattgg gaaatataca
     3481 taaaataata aattgttcat atttatggtc aaaatcaatt catgttcata ttggcaagac
     3541 gatttattat aatccctgta tattatcact gtaaaaccgg ctggatggtt ttacagatta
     3601 ttcactgaac aagagttcct gaaagagggg gttggatatg aaaaagattc ttatcatcac
     3661 catggcgatt ttcatgatgg cgacggtcgc agcatcggcg aagacatacg atctggtgct
     3721 ggtacacggg ctcaccaaca agcatcagtg gagcgatgcc ttcctggaca aggtcgcttc
     3781 gatctgggga tcgggcaatg tgtatgtcat ctatacgaac gaatcgacgc gcgtgtggac
     3841 gcgcagcatc aacggccgca ccatctactt ctgcggcgag aacgacttca gcgccggcga
     3901 cgacaccatc tacgaccagg cgacgctgat gcggacgaag atcaccaagc tcaattcgtc
     3961 ctacgggctc agcgccaagt tcaacgtcat cgcgcacagc atgggcggat tggtgagccg
     4021 ctgctacatc aagcgattcc cgaacacggt cgcggggctg gtgacgctcg gcacgccgca
     4081 ccagggctct ccgctggcga atgtggcggc atggctgtcg ttcttcatcg gcgcggaggc
     4141 cgcggccaat cagctcaagc ccgcgtatgt gaagaacacc ttcaatccag cataccccgt
     4201 ggtcggcgca ccgcttgcgg acagcggaaa gatctatacg atcggcgggg acggcgacgg
     4261 ctgggactgc tggggatggg gcggcgagtt gcagcttggc tgggacacgc tcaccacggt
     4321 atattggaag gacagcgacg gggcggtcgc gcgcggcgac gagcagatca gcggggccac
     4381 gcatgttgcc accttctgga gctacgatca catggaactg gttaccaagg ccgatgtggc
     4441 cacgaaggct gccgcatatc tccgataaca tacgcaccga gagcctgcat tgagatcgcg
     4501 accccctgga gacagggggt ttcgcgtatt cgctccacgc gttgatgcgc ttagcggatc
     4561 ttctccgcga taaacccgcc gacgatgtcg ccgttgtgct tcaccgtccc cgcgaagctt
     4621 cgtttccgtt cgatgtaata tccctcgaac tcgaagcgct cccggccgct ctccttgaga
     4681 ttaacgtcga tggtgaagga gagcccggtg atgcgcgtac gctttttttc atcgtcgaat
     4741 cgctgttgaa ggcgataggc ttcgatgggc agctccttca cggtgtccca gtatcgggga
     4801 acggtgatgc tcgctgccat cgtcagggtg gggtagatgt ccttcaccgg cacgacggtt
     4861 atgcttttta tagtgaggat tttatcgtgc cacatggtcc ggttcccgct gatgaggacc
     4921 cggtacgatc cggcgatgag cgcgatctcc gcccggtccg gtcccggata gggttccttc
     4981 catccgcctc cgtcgcgata tttttgcaca accggaccgg tattcgcgaa tgttggggtg
     5041 atgataacga tcgcgagcaa caaactccac gcaaggaatt tcactggcgg cacctccgtc
     5101 gtattgatcc gacattcccc attcgatgtt gcctgccgat gggaattccc gtaagcgttt
     5161 cgcagaaagc ggggggcgtc aatgaaatat tggatgctga aaaaacccca acctggtgtc
     5221 cccttcataa gggggaggtg tccgaaggac ggagggagtg gcgatcgatg gtgcagtttt
     5281 cagaaggaga agcgtgtcgc gagggcaggg atgcccgttc gcgaaatcgc catgacggcc
     5341 tgccatatca ttcattcgct tgcggctatg tgactgccgg gccgggctgt tagggatgtt
     5401 cggctgggtc cccgtccccc gcgagcaatg aacagtggta catgaagatc ccggcggcca
     5461 cgcccacgtt tagcgaatcg ccgcgggcga tggggatggt gatcgcgcga tcgcagcgat
     5521 cgcgccaggc cgcatcgagc cccgaggcct cgttgccgaa tacgagcgcg tgcttcccgc
     5581 atgcacggaa cgccggtatc ggccgcgcat cggatgccag gacggtggca tataccgcga
     5641 aaccatggtc gcgcagcgcc ccgacggcgt catcttcgcg cccgatggcg agcacgggga
     5701 gggtgaaccc ggcgcccatg gacgcgcgca gggcgcgccg gctccagggg tcagagcctc
     5761 cgggagtacg ggcgatggcg tcgtaatcga gggcggccgc cgagcgcatg atgctgccga
     5821 gattgtcgtc gctgtcgagc cctgcgcaga tgacgaggcg gcgcgccggc ggattgcttg
     5881 cgagaaagcg ttcgatcccc agcggcgccg ggcgcctccc cgccgtcatc accccgcgat
     5941 ggaaggggaa gccgacaagg cgcgcgatct cgctttcgtc cgcgatcacg agcgggcagc
     6001 gcccctgggc cagcggcgcg atgcgttccg ccatgcgcgc gctgcagagc gtcgcgatca
     6061 tctcaaggcc cgaggcgagc gcccgttcta cgagaaaccc gccctcggcg atgaaaatcc
     6121 cctcgtccct gagcgcgcgg tccttcaggt tcgcgaagac gctgatgtcc ggactgttca
     6181 ttgatgcatc gtgtaacatg gggcgcttta ctgcaaggac aacatggcgt agcggagctg
     6241 gaattaaaac tgtcactgtt ttattaaacc cctatccccc ggcccctttc cccttcgtta
     6301 gggaaaaggg gggtgcggaa gatcattatt aacacacctc cccctaacga agggggaggt
     6361 cgggaggggg tcaataagtc gatgttgata aaaaaatttc aaattttgta caagttttgc
     6421 aggagaatat tttattttta agtacgaata cataattatg tcaataagtc tggtcaaaat
     6481 caatttaaat ttccattggc cagacgattt gagacaaatt ctatattatt ccaatatacc
     6541 gtccggatgg ttttttgatt ctcggatatg gtgaatacaa agttttatta accaagagca
     6601 gtctcaagca aatctttctt taggaggggt catgaaaaag agtattgcgg ttgtagcgat
     6661 tgtagtgatg gcagtgtgtg caacagcggc gttcgcgggg ggatcgaccc ccacctgtaa
     6721 caccaagtat ccgatcgtgc tggcccacgg catgggcgca tcggcgcaaa ttctcggcat
     6781 catcgattat tggaacgaca tccccggggc cctcagggac aaaggggcga aggtatatat
     6841 cacttcggta aacggcatgg acagcacggc caacaaggcc gccagctgga agagtcagtt
     6901 cctgaacatc aaggcggtga ccggggcggc caagctcaac gtgatcggcc actcccatgg
     6961 gaccatctac acgcgctatg cgatatccaa cctcgggctt tcttcgtatg tcgccagcca
     7021 caccagcatc gccggtccgc atcgcggaag cgccgtggcc gacgtggtcc tgggcgtgat
     7081 tcccaatccc tgccaggccc tcgtgggtgc gaccctggat ttcgtgtacg ccttcgtctt
     7141 cggcgacaag aacccgagca gcctccagaa cggaaaggac ctggtccgca gctacatgat
     7201 aaacaccttc aatcccaata cgcccaacaa gagcggcatc tactatcaga gctgggcggg
     7261 caagatcaag acgatggcca tcgacgcgcg caactggtat ttcctgatca cctggcccat
     7321 cctcaagtac tatgaaggcg acaatgacgg cctggtgtcc gtgacctccg cgaagtgggg
     7381 caccttccgt ggcgtgatcg agggcgcgtg gtactcggcg ggcgtcgacc atctgaacat
     7441 ggtggaccag cccttcggga tcacccccgg ctttgacgcc cccgagcagt ttgtgagcct
     7501 ggtttccgag ctcaagagca aagggtacta gtccccggaa ccgatccatt ggctcaaacc
     7561 aaagagcccc cgttgatgcg ggggctcttt gtgtatcctc tttaaacgcg catcgctttg
     7621 atcatttgtt cctcgaatgg attttaacat aacactaatg cgtttagtgt tgataaatgc
     7681 actgaaattt tttcaaaaaa gcccctgcgg gcgaatattt ttcttgaccg accgtgtagg
     7741 tttttttcat atacatgaac tagttaataa aaatagatca cgttaaagta ttatttcgag
     7801 gtgtgatatg cgtcactatg tacagagtct tcttgttatt ttttgttcca ttatgatttc
     7861 attcatcgct ttatccggct gcggtagcaa gggaggtgat ggcggcggcg gaggcggtgg
     7921 cggcgcaggc agcggcgcga gcaacggcac ctataccgag gtcaatacga ccgcgaaaac
     7981 cgggaccatc atcggcgcgg tgagcggaca ggattaccga gtcgccggcg gtgtgcagtc
     8041 gccgaacaac gattcgtatg cgcttggcgg agcgaccatc accatcaccc atgaaagcct
     8101 catcgagggc ggcaccctgg tcgtgaacgt cccctcgagc ggcgactacc agatcgtcct
     8161 gccggccggc accggctaca ccatcgtgtt cgcgatgccg gggtacgaat cggtgaccta
     8221 caccgatgtc gagatcatcg ccgaccagac catcaccctg gagacggccg tcctcaagaa
     8281 taatgcgctc acagtggtcg tcgatgggac cgtgtccaat aacgggacgg gcctgagcgg
     8341 cgtgcaggtg accatccagt gcagcggcga caactgcgac ggtatcgccg atgtgaccac
     8401 ctacaccgaa aacgacggca cgttctacgt gaaccttccc gcgggacatt gctatacggt
     8461 cacttatgat cttgccgggt accaaagcgc gaacggcggc gagttctgca tcaccgacaa
     8521 cgggacggtc acgggcttca atggcactgt gacgctcaat ccgatcgtga ccaccgggac
     8581 gctcgcgggg atcatcacgg gcgagacgct cgctggcggg accgcgggca tcaaggcgac
     8641 cattcagatc agcaaggacg gcgcggtcat cagaacctac gagaccgacg acgacggaaa
     8701 ttacagcatt acccttgacg gcgggagcgg gtatacggcg acggtcgtcg cgctcggcta
     8761 tacgacgatc acgaccggtt ccttctcgat cgcagccggc gaaacgaaat cgatcgaggg
     8821 cgcctcgctt cgcctcgtga cggggacgct catcggctcg gtgaccgacg catcgggcgc
     8881 tgcgatgggc ggcgtgtcca ttagcatcta ctatctggac ggcaccgttg tgcgggcgtc
     8941 gaccagcacc aatgataacg gttcctttag catcgtcctg ccggtcatct cgtcgcagag
     9001 cggcgagggc gactactatc gcgtcgtgtt ccagaagccc ggctacgtgg ataccgttta
     9061 tcatgtgtac gacatctttc agagcctcga ggtttcgctc cagccggtgg tcgtggtcat
     9121 cgacggctac gggatcgtgt ccggttctat catcagctcg gttgacgggc gcggcgtgtc
     9181 aggcgcggtt attaagctcc ggagcggcat cgatgtgaaa accggtgtgt atatgagcgt
     9241 cagtggcgcg acggttacgg caaccagcga tgcgaatggc gcataccgga tacaaggcct
     9301 tcccgccggt agctacacgg gggaggtggt caagtccggc tataccaccg gctatttcac
     9361 tatcgtgtgc gtcaaggatg tcgagacacc ggatcagcgt tgcatactca gccccaccga
     9421 cagtgtatcg ccgaccagcg gacgtatcga ggtggtcctg aaatgg
//
LOCUS       DAORHF010000142         9391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6740_length_9391_cov_5.021958, whole genome shotgun sequence.
ACCESSION   DAORHF010000142 DAORHF010000000
VERSION     DAORHF010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9391
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6740_length_9391_cov_5.021958"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="PKJ16_11915"
     CDS             complement(<1..725)
                     /locus_tag="PKJ16_11915"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNV47740.1"
                     /translation="MGTENEKTGTDEQSPKKKASPDLKALKCSACGGDITKTHRVEGS
                     GEDEEYIPIARCLNCGKEYDQHSQEYYEYYADMFRRDLDKSVFKLGIKGTIDGVEYEI
                     IGHIRYQDEDEWEVETWDEWLAISSEGTYRYFVEEEGEIYSFEEYTPNSIDLATDPSS
                     IEFEGKKVSRERTRYTARIVFAEGELPWQPEIGEPITVYDFKHQGFYITIEQDEDEVS
                     ITRGTVVPHGVIIDAFRGAEFKDA"
     gene            complement(731..1336)
                     /locus_tag="PKJ16_11920"
     CDS             complement(731..1336)
                     /locus_tag="PKJ16_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HNV47741.1"
                     /translation="MAAYTCTSCGAPIEVKNRFSKVVVCDYCGTHHKVTVDGLDPTGK
                     HPKLADFPSLFQVGSRGTILGKPFTAQGRLRYKYDGGHFDEWFIEYDGEPAWFAEDEG
                     TFTLFTELIEAIDIPLDMSSLRAGQNIMIGDKKVMIKEKGSAVIEGGEGELFHYTEPG
                     TKVTYIDGVWNGKKTSIEFDEDEIELFIGRPLLKRDIVVEG"
     gene            complement(1344..2465)
                     /locus_tag="PKJ16_11925"
     CDS             complement(1344..2465)
                     /locus_tag="PKJ16_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HNV47742.1"
                     /translation="MALLDVVKWENKEGEIIYRFPEGSISMGAQLIVMEHQEAVFFRE
                     GRALDSFGAGRHTLKTGNIPILEKLVNLPFGGKSPFPAEVYFLNKTEIPNLKWGTRQP
                     MQLMDPTFNLAIPVRAFGNYSIRINDSKPLLMMAIGTWKAFTTDAIGTALRDQIIIPK
                     IQDLISETMIKQNITIFKMTAYLDEIATAGKAKISEDFTSFGIELVRFAVESINAPEE
                     DESVKRLKKALADKAEIGIMGQDDYKMKRTFDTMEKAASSNGAGGMMGAGMGFGMGQQ
                     MGTMMQGAMGSAQGGQGAAVKCPSCGVANKPDAKFCSECGKEMIVTRACPKCQAKIKP
                     DAKFCPECGASLQDVKCAKCGAAMQAGAKFCPECGEKSA"
     gene            complement(2466..2990)
                     /locus_tag="PKJ16_11930"
     CDS             complement(2466..2990)
                     /locus_tag="PKJ16_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47743.1"
                     /translation="MSPDINDIKRNIEGQRDLLSRIITAVPGFQGYVETGEKYDADRV
                     IRNFMVDKILAFKQTVSGAMRDMQKSVDHDVLSRLDSLGTVLERILKKCQFADYGKKS
                     FTGVSMTPEQQDKLLAYDWNLIFRLDEIGTEFDRLSGVEPGAVSGIVADILKKLNEFE
                     KEIDGRKSVILEVL"
     gene            complement(3130..4812)
                     /locus_tag="PKJ16_11935"
     CDS             complement(3130..4812)
                     /locus_tag="PKJ16_11935"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47744.1"
                     /translation="MSNKSMMYASPVPLRNDSFQQAPADAITAAVDAAPSDDAAGWFA
                     RANAHLHLNLYDKALEDYDRALALDPADAAAWYNKSVALSALNREDDAIKALERVIAI
                     EPTDTEARRRRAGLLKYGGRFDEALNEYHDIIAITPNDAQTWYNLATLLEYDLGMEEE
                     ALEAYDRSTAIDPSRPWVWRDRGMLLMSMRRYDDAIASYDRVTELMPVDATSWHMKGE
                     AFRKIGDLARARDAFAEHDRLVREDHEEALQRATDDCAKSPGDTRPLIRKAGALRALR
                     RLEDALAIYDELAAREPGSREHASNRAAVLAEMGRYEEAIAALDSAIALDPKRAYSHF
                     DKALVLEKMERLEDALASYDAALRGDPANGYFGYCKAELLRQLSRFDHSITALDAVLE
                     INPEHEEALNLKGLVYHDKKEYDRALECFDQILEFRPESNVAWINKGWVTEKISGAID
                     EAIFCINKALEIEPDNVDAWINKGYLMYKLQRYDDAVSCSTRAIEFEPTSAIAWFNRA
                     CFYAVMGDRDRALSDLAEAIRLDPANRADARTDEALAALRDDPDFKKLIDLP"
     gene            complement(4905..4976)
                     /locus_tag="PKJ16_11940"
     tRNA            complement(4905..4976)
                     /locus_tag="PKJ16_11940"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4942..4944),aa:His,seq:gtg)
     gene            5350..6222
                     /locus_tag="PKJ16_11945"
     CDS             5350..6222
                     /locus_tag="PKJ16_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNV47745.1"
                     /translation="MKGEEHVITGDEPLSDICFFGVAPEKSGSLRGARDFPSFPANRR
                     GIAQRVHLVVFDISNGGLMKSVLNPTAPERDVLVKEIAAASAPFAGVMIDFEAVPPDM
                     RAPFIEFLTRLRSSLPKNSILSVALPARRMQVNDAYDYPAISAIVDRVFIMAYDQHWS
                     TSKPGPVAAIGWCDAVAAFAQKNVPESKLVMGLPLYGRVWQDRYLGRSVRHSDVESIL
                     KRKRRLKPRIHHDRGPSLSYRETIHVTIYYEDKDSIARRLAMYQRRGIGQVGFWRIGQ
                     GPRGMWSLMELKPI"
     gene            6696..7466
                     /locus_tag="PKJ16_11950"
     CDS             6696..7466
                     /locus_tag="PKJ16_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HNV47746.1"
                     /translation="MTVLKCSLSAAIRAVRNQFMLFVVIAFALLSIGLLIGAAAKSAM
                     ARSIDRVAASDAVKKAIQKFESTPESDLDNTHAPAVGPRNARIAITIFTDYTCDHCAA
                     ASKLFGKMMAAYPGLISITYRSYPLSDGCGTIPDVNASTEASCIGALAAQCAHRQHRF
                     IDMHERLYRDNERGIAHTSKSVNVIAKSIGLSANEFAGCLSSPATQSQLALEAKKGDA
                     LGIEGTPTIFINKRKVIVGLPPESVVRGLIDYLRSRGK"
     gene            complement(7503..>9391)
                     /locus_tag="PKJ16_11955"
     CDS             complement(7503..>9391)
                     /locus_tag="PKJ16_11955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47747.1"
                     /translation="SLQFFKRMVDEAPFAAAIADFENEFIGRIQYVNREVVRLTGYEL
                     DEIGTATNFMDLVYPDPAYRAFVFENWQKAILRAHGDQGSIQPQEYRIRRKDGAYAET
                     MWMYSALGDKSVILVLDITERRMAEEAVRSSEKKYRSLYESMLDGYVRIDADGRIVEC
                     NAAYREMLGYDENEIMTLTYSDVTPERWHELQDRIIREQVLTRGFSDLYEKEYRRRDG
                     SVFPVELRTYRILDERDQYAGMWAIVHDLTKRKQAEQELLKASKIESLGILAGGIAHD
                     FNNLLMGIVGNISLVRNDIEAQSRNAAILGEAERASLRARDLANQLLTFSKGGVPVRQ
                     LVSVKSIIRESAEFVLSGSNVKCVFAIADDLWNAHIDEAQISQVINNIIINARQAMGD
                     RGRIDVRADNVHVDSLSHVSLDPGDYVRVSVTDKGCGIAAEDLQSIFDPYYTTKKDGN
                     GLGLTITYSIVKNHGGCVTVDSAVGKGSTFTVYLPASKSPVLANEDGAVVSFKGSGRV
                     LVMDDDESVLSVAAEMLRVFGFDPELAHDGEEAIERYREAQRTGRPFSIVIMDLTIPG
                     GMGGKEAIAVLREIDPKVRAVVSSGYSNDPIMSNYRDYGFMGIVAKPYRLEELRRMLQ
                     GLAL"
BASE COUNT         1802 a         2924 c         2602 g         2063 t
ORIGIN      
        1 gcgtccttga attctgctcc gcggaaggcg tcgatgatca ctccgtgcgg aacaaccgtt
       61 ccccgggtta tggaaacctc gtcctcgtcc tgctcgatgg tgatatagaa accctgatgt
      121 ttgaaatcat agacggtgat cggttctcca atctcaggct gccaggggag ctcgccttcg
      181 gcgaagacga tgcgggcggt gtatcgggtc cgctcccgag aaaccttctt tccctcgaat
      241 tcgattgagg aggggtctgt cgcgagatcg atagaattcg gcgtgtattc ctcgaaggaa
      301 tatatctccc cctcctcttc cacgaagtat cgataggtcc cctcggacga gatcgccaac
      361 cattcgtccc aggtttcgac ctcccattcg tcctcgtcct gataacgaat gtgcccgatg
      421 atctcgtatt cgacgccgtc gatggtcccc ttgataccca gcttgaacac ggacttgtcg
      481 aggtcgcgcc tgaacatgtc cgcatagtat tcatagtact cctgtgagtg ctgatcgtac
      541 tccttgccgc aattgaggca tcgggcgatc gggatgtact cctcgtcctc gccgcttcct
      601 tcaacgcgat gggtttttgt gatatcaccc ccgcaggcgg agcatttgag ggccttcagg
      661 tcgggcgatg cctttttctt cggggattgc tcgtcggtgc cggtcttttc gttttcggtg
      721 cccattgggc ttacccctca acgacaatat cccgcttcag gagcggtctt ccaataaaaa
      781 gctcgatctc gtcctcatcg aactcgattg aggtcttttt cccgttccac accccgtcga
      841 tataggtaac ctttgttccc ggctcggtat agtggaacag ttccccctcg ccgccctcga
      901 tgaccgcgct ccccttctcc ttgatcatga ccttcttgtc gccgatcatg atgttctggc
      961 cggcccgcaa tgacgacatg tcgagcggta tgtcaatcgc ctcgatgagc tcggtgaaga
     1021 gcgtgaaggt gccttcgtcc tcggcgaacc atgccggctc cccatcgtac tcgatgaacc
     1081 attcgtcgaa gtggccgcca tcgtacttat agcgaaggcg tccctgggcc gtgaagggtt
     1141 ttcccaaaat ggtcccgcgg cttcccacct gaaaaagcga gggaaagtcc gcaagcttcg
     1201 ggtgtttccc cgtgggatcg agtccgtcga ccgtcacctt gtggtgggtg ccgcagtagt
     1261 cgcagacgac aaccttgctg aatcggtttt ttacttcaat gggcgcgccg caagatgtgc
     1321 aggtatacgc tgccatcgcg cacctatgcg gacttttcgc cgcattcggg acagaacttc
     1381 gccccggcct gcatcgccgc gccgcacttg gcgcacttta cgtcctggag gctcgcgccg
     1441 cactcggggc agaatttcgc gtccggtttg atcttcgcct ggcatttcgg acacgcgcgg
     1501 gtcacgatca tctcctttcc gcattcggaa cagaacttcg cgtccggctt gttggccacg
     1561 ccgcacgacg ggcacttgac ggccgcgccc tgcccgccct gcgcactgcc catggcgccc
     1621 tgcatcatgg tccccatctg ctgacccatg ccgaatccca tgccggctcc catcatgccg
     1681 cccgcgccgt tgctcgatgc tgccttctcc atggtatcga aggtgcgttt catcttgtaa
     1741 tcgtcctggc ccatgatgcc gatctcggcc ttgtcggcca gcgccttctt gagccgcttg
     1801 accgactcgt cctcctccgg cgcattaatc gattcgacgg cgaaccgcac cagctctatg
     1861 ccgaagctgg tgaaatcctc gctgatcttc gccttgccgg cggtcgcgat ctcgtccagg
     1921 tacgcggtca tcttgaaaat ggtgatgttc tgcttgatca tcgtctcgga gatgaggtcc
     1981 tggatcttcg ggatgatgat ctgatcgcgc aacgcggtgc cgatcgcgtc ggtcgtgaac
     2041 gccttccagg tcccgatcgc catcatgagc agcggcttgg aatcgttgat ccggatggaa
     2101 tagttcccga atgcgcgcac ggggatcgcc agattgaagg tcggatccat gagctgcatc
     2161 ggctgtcgcg ttccccactt gaggtttgga atctcggttt tattcaggaa atagacctcg
     2221 gccggaaagg gcgatttccc gccgaagggg agattgacca gcttctcgag gatggggatg
     2281 tttccggttt ttagcgtatg acgaccggcg ccgaaggaat cgagcgcgcg tccctcccgg
     2341 aagaaaacgg cttcctggtg ctccatgacg atgagctgcg cgcccatcga gatcgaccct
     2401 tcggggaacc gatagattat ttcgccttct ttattctccc atttaactac atcgagcaac
     2461 gccatctaga gcacctccag gataacggat tttctcccgt caatttcctt ctcgaattca
     2521 ttgagctttt ttaagatgtc cgcaacaatt ccggaaaccg cacccggctc gacgcccgac
     2581 aagcgatcga actcggtccc gatctcgtcc agccggaaga taaggttcca gtcgtaggca
     2641 agcagcttgt cctgttgctc cggcgtcatt gaaacgccgg tgaacgattt cttcccgtaa
     2701 tcggcgaact ggcatttctt caaaatccgc tctagtaccg tgccaagcga atcgagacgg
     2761 gacaggacgt cgtgatcgac gcttttctgc atgtctcgca tcgctccgct tacggtctgc
     2821 ttgaaggcaa gaattttatc gaccataaag ttccggatca cccgatcggc atcgtacttc
     2881 tcgccggttt ccacatatcc ctggaatccc ggcaccgcgg tgatgattct gcttaacagg
     2941 tcgcgctgac cctcgatgtt gcgcttaatg tcgttgatat caggggacat ggctcctcct
     3001 ttgctgcatt gaaatatacg tcagtcgcga taaaagtcaa gagttatatt taaatatttt
     3061 cttcgaccgc tctcggtacg tatttgagaa cgcaaccgtc tatttgttac aggatgtcca
     3121 aggaatccct tatgggaggt caatcagctt cttgaagtcc ggatcgtcgc ggagcgcggc
     3181 cagcgcctca tcggtccgtg catcggcgcg gttggcggga tcgaggcgta tcgcctcggc
     3241 gagatcggaa agcgcccgat cgcgatcgcc catcaccgca taaaagcagg cgcggttgaa
     3301 ccaggcgatc gccgatgtcg gctcgaactc gatcgcgcgg gtcgagcagg acacggcgtc
     3361 gtcgtagcgc tggagcttgt acatcaggta tcccttgttg atccacgcat cgacgttgtc
     3421 gggctctatc tcgagcgcct tgttgatgca gaaaatcgcc tcatcgatcg ccccgctgat
     3481 cttttccgtg acccagccct tgttgatcca ggcgacattg ctctcgggcc gaaactcgag
     3541 gatctgatcg aaacactcga gcgcgcgatc atactccttt ttatcgtgat agaccaaccc
     3601 cttgagattg agcgcctcct cgtgctccgg attgatctcg agcactgcgt ccagggccgt
     3661 gatcgagtgg tcgaatcggg agagctgccg gagcagctcg gccttgcagt agccgaaata
     3721 tccgttcgcg ggatcgcccc gaagggccgc atcgtacgag gccagggcgt cctccagccg
     3781 ctccatcttc tcgaggacaa gcgccttgtc gaaatgcgaa taggcacgct tcggatcgag
     3841 cgcgatcgcg ctgtcgagcg cggcgatcgc ttcctcgtag cgccccatct ccgccaggac
     3901 ggccgcccgg ttgctcgcgt gctcccgact gcccggctcg cgcgcggcga gttcgtcgta
     3961 aatcgcaagc gcgtcctcaa ggcggcgaag cgcccgcagg gctccggcct ttcgaatgag
     4021 cgggcgcgta tcccccggac tcttggcgca atcgtcggtc gcccgctgca acgcctcctc
     4081 gtggtcctcg cggacgaggc ggtcgtgctc cgcaaaggcg tcgcgcgcac gcgcgagatc
     4141 accgatcttg cggaacgcct cgcccttcat gtgccagctg gtcgcgtcca ccggcatgag
     4201 ctcggtcacg cggtcgtagg aggcgatcgc atcgtcgtac cgacgcatcg acatgagcag
     4261 catgccgcga tcgcgccaca cccacggacg cgacggatcg atcgccgtgg agcggtcgta
     4321 cgcctccaac gcctcctctt ccatgccgag gtcgtattcg agcaacgtcg cgagattgta
     4381 ccaggtctgg gcgtcattgg gagtgatggc gatgatgtcg tgatactcgt tcagcgcctc
     4441 atcgaagcgc ccgccgtatt tcagcagacc ggcgcgtcgg cggcgggcct ccgtatccgt
     4501 tggctcgatg gcgatgacgc gctcgagcgc tttgatggcg tcatcctcgc gattgagcgc
     4561 gctcagcgca acggacttat tataccatgc cgcggcgtcg gccggatcaa gcgccagcgc
     4621 acgatcgtaa tcctctaacg ccttatcata aaggtttaaa tgcagatgcg cgttcgcccg
     4681 tgcgaaccat cctgcggcgt cgtcgctcgg cgctgcgtcc accgccgccg ttatcgcgtc
     4741 ggcgggcgcc tgctggaacg aatcattccg cagcggaacg ggacttgcgt acatcatgga
     4801 tttattcgac atgcgttatg tcctcgcacg gcatgcgtca tgcaatcggg agtgagtgag
     4861 aataaaaaac ccgcgttcgc gtgcgacgaa tgcgggcttc gatgggtgga tgacggggat
     4921 cgaacccgca acaactggaa ccacaatcca gggctctacc attgagctac atccaccgtg
     4981 tatatgtccc ccggaggcag cacaaaactc tccgacggaa gcgcaagggt tcatattcat
     5041 aaactagtaa aaaaacatta aaaagctgtc aacaaatatt tcgaagcagc agtaatgcgc
     5101 tttttttatt gcaaataaat cctcatcccg gtacgtgaca atcgaatcgc accaagatga
     5161 aatgatcgcc aatcacgaac caacggttat ccaaatatgc catcacgatc gcgcctgata
     5221 ggagcaacgc tgagttgcgc cctcgccatt gcatttctct cgataccatc attcgccgac
     5281 gcccggtcca aacgaaaacc gacgcgcccg cgtcccccgg tcgcattcag cgagctttgg
     5341 ggattcgtca tgaaagggga agaacatgtg ataacgggcg atgagcccct gagcgacatc
     5401 tgctttttcg gcgttgcgcc cgagaaaagc ggctcgctgc gcggcgcgcg cgatttcccg
     5461 tcctttccgg cgaaccgccg cgggatcgcg caacgggtcc atctcgtcgt gttcgacatc
     5521 agcaacggcg gcctgatgaa atcggtcctc aatccaaccg ctcccgagcg cgatgttctc
     5581 gtcaaggaga tcgccgcagc gagcgcgccg tttgcgggcg tcatgatcga cttcgaggcc
     5641 gttccgcccg acatgcgcgc tcccttcatc gagttcctca cgcgccttcg ctcctcactc
     5701 ccgaagaatt cgatactcag cgtggcgctc cccgcccgcc gcatgcaggt gaacgatgcg
     5761 tacgactacc ccgccatttc agcaatcgtg gaccgcgtct tcatcatggc atacgaccag
     5821 cactggagca ccagcaaacc cggtccggtg gcagcgatcg gctggtgcga cgccgtcgcc
     5881 gcattcgcgc aaaaaaatgt tcccgagagc aagctcgtta tgggccttcc gctctacggc
     5941 cgcgtgtggc aggaccgcta tctcggccgt tccgtgcgcc acagcgacgt tgaatcgatc
     6001 ctcaagcgaa aacgtcgcct caaaccccgc atccatcatg accgtggtcc gtcgctctcg
     6061 tatcgcgaaa ccatccatgt gaccatttac tacgaagaca aagactcgat cgcacgccgt
     6121 ctcgcgatgt accagcgccg cggtatcgga caggtcggtt tctggcggat cggccaggga
     6181 ccgcgcggca tgtggtcgct gatggagctg aaaccgatat gaggcggtta ctctcactgt
     6241 gtatcgcggc atccgcgctg gcgggggccc tcctgagcat tttgctcatt ctggaatatc
     6301 acggcataac gctttcagta tcggaaagcg tttgccgttc cggggcgagc ggtttcaacg
     6361 cctgccaaaa tgtcgccaga agcgaatacg ccgcgataca cggaatccca ctcgttggcc
     6421 ccgtgccgac ggcggtgttc ggcctcctca tgttcggctt tgttttgtgc ctgtcgattc
     6481 tcgatcttgc acgcaacccg gagagcgctc aggatatcac ggcgcttatc gccctgatct
     6541 cggttataac gctgttcttg gacatcctat tgctgtacat catgacgtgc cggatcaagg
     6601 ccgtctgtgc gctgtgtcta atgacctaca ttgcgaatct atgcatcggt tgttccgcga
     6661 ttgcatcact ctgtattttt tacaaggagt cgtgtatgac tgttctcaag tgcagcctgt
     6721 cggccgcgat acgcgccgtc cggaaccagt tcatgctttt cgtggtgatc gcgttcgccc
     6781 tgttgtcgat cggcctgctg atcggcgcgg cggccaaatc cgcgatggcc cgttcgatcg
     6841 accgagtcgc cgcatcagac gcagtcaaaa aggcgatcca gaagttcgaa tcgacccccg
     6901 agtccgacct cgacaatacg catgcacccg cggtggggcc gcgaaatgcg cggatcgcca
     6961 tcacgatatt caccgattac acctgcgacc actgcgccgc ggcgtccaag ctcttcggaa
     7021 agatgatggc ggcgtacccc ggactgatat cgatcaccta ccgctcctac cccctgagcg
     7081 acggctgcgg cacaattccc gatgtgaacg catcgaccga agcctcgtgc atcggggccc
     7141 tggctgcgca gtgcgcccat cggcaacacc ggttcatcga catgcatgaa cgcctctatc
     7201 gagacaatga gcgcggcatc gcccacacgt cgaaatcggt gaacgtgatc gcgaaatcga
     7261 tagggctgtc cgcgaacgaa tttgccggct gcctgtcatc tccggcgacg cagtcgcagc
     7321 ttgcgctcga ggcaaaaaag ggggatgcgc ttgggatcga gggcacgccg accattttca
     7381 tcaacaagcg aaaggtaatc gttggacttc cgcccgagtc ggtcgtccga gggttgatcg
     7441 attatctgcg ttcgcgcgga aagtgaacgc gggggggggg tgcgccactc gtatcgcccg
     7501 tgctataatg cgagcccctg gagcatccgg cgtagctcct cgagccggta gggcttggcg
     7561 acaattccca tgaagccata gtcgcgataa ttcgacatga tgggatcgtt cgagtaaccg
     7621 cttgatacga cggcccgaac cttcggatcg atttcccgaa gcaccgcaat cgcctccttt
     7681 ccgcccatcc ctcccggaat cgtaaggtcc atgatcacaa tcgaaaacgg cctgccggtc
     7741 cgttgcgcct cgcgatagcg ctcaatcgcc tcctcgccgt catgcgcgag ctccggatcg
     7801 aatccgaaga cgcgtagcat ttcagcagca acggagagga cgctttcgtc atcatccata
     7861 acgagcacgc gccccgaccc cttgaaggaa accacggcgc cgtcttcatt cgcgagtacc
     7921 ggcgacttcg aggccgggag atacaccgta aaggtcgacc ccttcccgac ggccgaatcg
     7981 acggtgacgc atccgccgtg atttttcacg atggaatacg tgatggtgag ccccagtccg
     8041 tttccgtcct ttttcgtggt gtaataggga tcaaagatgc tctgcagatc ctccgccgct
     8101 atcccgcacc ctttgtcggt cacgctcacg cggacatagt cccccggatc gagcgacaca
     8161 tgcgagagcg aatcgacatg gacgttgtcg gcgcgcacgt caatccgccc gcggtccccc
     8221 atcgcctggc gggcattgat gatgatgttg ttgatcacct ggctgatctg cgcctcgtcg
     8281 atatgcgcgt tccagaggtc gtccgcgatg gcaaacacgc acttaacgtt ggaaccgctc
     8341 agcacgaact ccgccgattc ccgtatgatg ctctttaccg agaccagctg ccgcaccgga
     8401 acgcccccct tcgagaaggt gagcagctga ttggcgaggt cccgcgcccg aagcgatgcg
     8461 cgctccgcct cgccgagaat ggcggcgttc cggctctggg cctcaatatc gtttcgcacc
     8521 agtgaaatgt tgcccacgat gcccatcaac aggttgttaa agtcgtgcgc gatcccgccg
     8581 gcgagtatac ccagcgattc gatcttgctg gccttgagga gctcctgctc ggcctgcttg
     8641 cgcttggtga gatcgtgcac gatggcccac atgccggcat actggtcccg ctcgtcgaga
     8701 atccggtagg tgcgaagctc gaccggaaac acgctcccgt cccgccgccg gtactccttt
     8761 tcatacaggt ccgaaaatcc gcgcgtgagc acttgttcgc gtatgatgcg atcctgcagt
     8821 tcgtgccatc gctcgggggt gacatccgaa taagtgagcg tcatgatctc gttctcgtcg
     8881 tatccgagca tctcacgata cgcggcattg cactcgacga tgcggccatc ggcgtcgatg
     8941 cggacatagc cgtccagcat gctctcgtag agggagcgat atttcttctc ggagctgcga
     9001 acggcctcct cggccatgcg ccgctcggtg atgtccagca ccaaaatcac cgacttgtcg
     9061 ccgagggccg aatacatcca catggtttcc gcataggcgc cgtccttgcg ccggatccgg
     9121 tattcctgcg gctggatgga gccctgatcg ccatgggcgc gaaggatcgc cttttgccag
     9181 ttctcgaaca caaacgctcg gtaagccgga tcgggataca caagatccat gaagttcgtc
     9241 gcggttccga tctcatcgag ctcatatccg gtgagccgca ccacctcccg gttcacatac
     9301 tggatcctgc cgatgaattc gttctcgaaa tcggcaatgg ccgcggcaaa gggggcctcg
     9361 tccaccattc gcttgaagaa ctgcaggctc t
//
LOCUS       DAORHF010000143         9321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6810_length_9321_cov_5.477552, whole genome shotgun sequence.
ACCESSION   DAORHF010000143 DAORHF010000000
VERSION     DAORHF010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9321
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_9321_cov_5.477552"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..533)
                     /locus_tag="PKJ16_11960"
                     /pseudo
     CDS             complement(171..533)
                     /locus_tag="PKJ16_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002766081.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(643..1188)
                     /locus_tag="PKJ16_11965"
     CDS             complement(643..1188)
                     /locus_tag="PKJ16_11965"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HNV47748.1"
                     /translation="MKTRKYVWLSIGMVLLCAMQIACQQTSVQFVDRTQIEETVNGLF
                     IGVDERDWTRVKNTMSGTVLLDYTSMAGGKPATLAPDAIIDSWKGILPGFDATHHQLG
                     NFVVTRKADAVMVFCYGTATHYLKNKSNNNVWAVVGTYEFVLKKVEGLWKISSMKFDL
                     KYIDGNTSLPSLAMDRAKGKK"
     gene            complement(1327..2337)
                     /locus_tag="PKJ16_11970"
     CDS             complement(1327..2337)
                     /locus_tag="PKJ16_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002699897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV47749.1"
                     /translation="MGAKKYQTRVFLVVSILGMSMTFFLHCAKNVTQDKGRVSFSSEG
                     STLKSDIYYPKSYDRRKKAPAVIVVGSWTTVKEQMAGLYAQRLSEQGLIAVAFDFRNY
                     GGSEGDIRFYESPRMKIQDIRNAVTFLTRFPGVDAQRVGVLGICAGAGYVLQAASEDQ
                     RIRSAVTVAAWLHDAEAVKLFYGGAAGVQAKREAAQKATRAYQKTGAIAYVPTISTTD
                     KNAAMYGPYDYYLNPRRGAIREWSADKFAVMSWEDWLTFNPMPSARKITVPVLMIHSD
                     GAVLPGYTRTYFNSIPGAKKRLHWVASDKQSPMGQFDFYDMPDKVSMAVREAAAWYAH
                     SL"
     gene            complement(2654..3553)
                     /locus_tag="PKJ16_11975"
     CDS             complement(2654..3553)
                     /locus_tag="PKJ16_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV47750.1"
                     /translation="MKTILQFVEQSTGSRSSCGDPLEIELSSRDLGWGGILLEKGWSP
                     HFFPSNIITPYFYFALAIEERFKWSAESRGELIDLKTEPGEIWMNPPNIPFTHIINDP
                     CHFIIFTIDEKTLLAHFEGMLPEQELQFLQNYNVADPSLEYFVRLFCNEASIRGRNGI
                     QYLQGLIKLFSTYYIRNYSNYADLVSKKSPSPRIHDGDIGSITRFIRENIERTIAIED
                     LAEICRLSKFYFLKEFKKHTGITPYQYILKVKLERSKELLREPGIQIIDVAYKLGFSD
                     QSHFANAFKKHFGYPPGAYKSSE"
     gene            complement(3670..3993)
                     /locus_tag="PKJ16_11980"
     CDS             complement(3670..3993)
                     /locus_tag="PKJ16_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV47751.1"
                     /translation="MKNPVKQNNVCPLFEAAFDILGKKWTGLILRVLSDGPKRFGDLS
                     GTICNMSDRMLIERLKELEAMNVVSRKVYDEIPVRIEYELTEKGKAFKPVLDEIQKWA
                     SEWCK"
     gene            4159..4458
                     /locus_tag="PKJ16_11985"
     CDS             4159..4458
                     /locus_tag="PKJ16_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HNV47752.1"
                     /translation="MKILALDHVRPGVTMDDIAPHLKDEARHAWQNYGNGLFRELYFR
                     GDRPGAVVIMECTDLDEAHAIMKKLPLVRKGLIELTFIPLGPFVPFASLFEPSHN"
     gene            4474..5070
                     /locus_tag="PKJ16_11990"
     CDS             4474..5070
                     /locus_tag="PKJ16_11990"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HNV47753.1"
                     /translation="MKLLFIKATPKSTIDSYTMKAAYAFLESYKKANAVHDVTVVDLY
                     REKMENLSRDILSELYYTADSPLQRYARQFAEADRYVFAAPMWTLSIPGILKTYLEYI
                     TLPGITFANTEKGPMGLLGGKGKKAIHITARGGIYSTGPAAEFEMGDRYLRTVLHFLG
                     IECVDTLAIEGTNFFKGTDLDVRINESLSAADRIGKSF"
     gene            5144..5629
                     /locus_tag="PKJ16_11995"
     CDS             5144..5629
                     /locus_tag="PKJ16_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV47754.1"
                     /translation="MISIINTRRSVRKFTSDTVPEGDIREILEAAMNAPSAMNEQAWQ
                     FIVLSGAVLDQFLAINRNTPKGAPIGILVCGDRRTSRDDYYVQDCSAATQNILLAVHA
                     KGLGSVWTTVFPDARKPVAQLLQLPDHVVPFSFTPIGLPATVPNAKSRYDGGKIHYNK
                     W"
     gene            complement(5685..6590)
                     /locus_tag="PKJ16_12000"
     CDS             complement(5685..6590)
                     /locus_tag="PKJ16_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47755.1"
                     /translation="MSHLHMVRIQPEDTRTALLEIQGAPRGFGLHARSSDECTLGFDD
                     APLYRIERSYPEERLFVETIPGGIGIRRERVAFENGADDWLRDSSCTDCSLFARLPDG
                     RYRIVKTQRNAVRSDSVLMGNEWTDAGTDTCCFDGCDRNEVHPRGRIDGPPVKEMFFS
                     TIHLCGGTPSSVRAQPLLAVIVPDYGMVWSGATGVLFFSFSRCDDVDDTGGAGILAIP
                     YAHDFIMHVNDRDDTDMKALRRIIDDFIRIIWSQYADRMMRIACDKGFYAMYLRAGIR
                     HSRHKISLHFLEQRHCPTPGLTGFP"
     gene            6858..8066
                     /locus_tag="PKJ16_12005"
     CDS             6858..8066
                     /locus_tag="PKJ16_12005"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HNV47756.1"
                     /translation="MNKTSRGLIVTLLFGISLFYAASFSTDAAQPNGTLHNAGRVDVL
                     HLRGSFREMGRQYGTLEKTKLRDFHADMKKLFVDKRGIAPARLNGIASELYDSYPQRF
                     REILVGMAETSGLSLDDHIFINAFEHFLFHPDLSRSSGGACSAIAAWRPHTADGNLIF
                     GRNYDFGDDVGAFKQYVNVAIFDPAGSGVATALVTFAGTLNATTQLNAAGLFLELNNG
                     GMSAGGLSHTNRVSTPIELFAMMLDSSSLRQIDLEMHTTNTRFSYIINVADANNAVSY
                     EWAPFGVKRIEGASGLLVSTNHFVGEWGLAPSTDAFFLTVTRRTNLLRLADRAKGALD
                     LAAMKGILNTDIRGGGATSYRVESIGGNPAPYTAYQVIAEPARRRVWIRLPGYQDWIA
                     LELGPLFGKR"
     gene            8240..8641
                     /locus_tag="PKJ16_12010"
     CDS             8240..8641
                     /locus_tag="PKJ16_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47757.1"
                     /translation="MIHHPSTVHWDTTLKQIFDEIDDYLEHTYGDLYHLRPNRPARGE
                     TSNKEHDGLFNVGSSFSAGFGSSLGRGFVVSVHLSTLQPVESAVYHQIMEVVIRLLRE
                     KLPERFPGRELKVDWDGDNLKIHGDLTLGEL"
     gene            complement(8658..>9321)
                     /gene="nth"
                     /locus_tag="PKJ16_12015"
     CDS             complement(8658..>9321)
                     /gene="nth"
                     /locus_tag="PKJ16_12015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HNV47758.1"
                     /translation="MSAAARRGAAMTARRARAVISRLLEHYGTPAPALAFGDRYQLAV
                     AVSLSAQTTDRQVNEVTPILFERYPDFERLSRARISDVERIVKSTGFFRNKAKNIVGM
                     ARAVVDEFGGALPGTREALMRLPGIGRKSANVILSIGFGVPAFAVDTHILRIANRIGF
                     IESDDPDAVEAAVTALVPERDWTAAHLVLITHGRSLCDARRPRCAECPVRSLCEYGST
                     PR"
BASE COUNT         2060 a         2790 c         2440 g         2031 t
ORIGIN      
        1 cataaaatta atactacttt tttcctcccc gcaaatattg tactgaatag ttacgaaatg
       61 tcaagaaaag ctgaccgcac ccgccggcat atgaatgacc ggacaacgtg ccgtatggtt
      121 ccatttttgg agttattttt gctgatgtta tgcggcggca gtgccatgga tcattcggca
      181 accatcggca ggttcacaaa atcatgcggg aatcccaccg tggttgcact cacgacatcg
      241 aggtattcta taccgagacg ctttaagctt gcctcgagag actgcaccaa acctttacgt
      301 tgattgccgc cacccttcgg atcggccggg cggccgttca atgtatactt tgtccccacg
      361 acgaagcgct cccgcccgga acggatgaat tcccccacat agcgcccgct ggtgccattg
      421 gtataatgat ttgcagtatc aatgtaatta ccgcccgccg caacgaacgc ctcgaatatt
      481 tttttacttt cgtccttcga cgcaccccag ccctattcct agttgaaggt catggtcacc
      541 agacagagct ccgaaaccct cggtcagctt ttcccgagaa gtttgtaccg cattgagacc
      601 tctatttgtt atccgtgcgt catcgattct tcctgcaacg tctcactttt tccccttggc
      661 gcggtccatg gccagggatg gcagacttgt attgccatcg atatacttga ggtcgaattt
      721 catggagctg atcttccaca gcccctcaac ctttttcagt acgaattcat aggtgcccac
      781 aacggcccag acattgttat tcgatttgtt tttcaaataa tgggtcgccg tgccatagca
      841 gaacaccatg accgcatcgg cctttcgagt gaccacaaag ttgccgagct ggtggtgcgt
      901 cgcatcgaat cccggcagga tgcccttcca cgagtcgatt atcgcgtccg gagccagggt
      961 cgccggcttg ccccccgcca tggaggtata gtccagcagc accgtgccgc tcattgtgtt
     1021 tttcacccga gtccaatccc gctcatcaac accaatgaac agaccgttca ccgtctcctc
     1081 aatttgcgtc cgatccacaa actgcacgga cgtctgctgg catgcgattt gcatagcaca
     1141 gagcaacacc atgccgatac tcagccatac atattttctt gttttcatat tacgctcctt
     1201 gattcaaata atctatcaca cattaattct ttttcacatc gtcactggtt cacacaacgg
     1261 aacatcttat acatcctgtc acaaaacacg gatatgcgtc cggctggagt tctcgattcc
     1321 tcgcctttat aagctatgcg cataccaggc ggctgcttcg cgtacggcca tgctgacttt
     1381 atctggcata tcataaaaat cgaactgccc cattggcgat tgcttgtcgc tggccaccca
     1441 atgcagcctc tttttcgccc cagggatgga attgaaatat gtgcgcgtgt atcccggcag
     1501 taccgcgcca tcggaatgga tcatgagcac cggcacggta atcttccggg cgctgggcat
     1561 gggattgaag gtcagccaat cctcccagct catgaccgca aatttatcag cgctccattc
     1621 ccgaatggcg ccgcgtcgcg gattgagata gtagtcatag ggtccgtaca tggccgcgtt
     1681 tttatccgtg gtactgatgg tcggcacata cgcgatggca cccgtcttct gataggcgcg
     1741 cgtggccttt tgcgcagcct cgcgcttggc ctgcacgcca gcggctccgc cgtaaaaaag
     1801 cttcacggcc tcggcatcat gcagccatgc ggcgacggtg actgccgatc ggatgcgctg
     1861 atcctcgctc gcggcctgca gcacatagcc ggcgccagcg caaattccca gcaccccgac
     1921 gcgctgagca tcgacacccg gaaagcgcgt tagaaaagtg accgcatttc tgatgtcctg
     1981 aatcttcatg cgcggcgact cgtaaaaccg aatatcgcct tcactgccgc catagttgcg
     2041 aaagtcaaag gccaccgcga tcaagccctg ctctgaaagc ctctgggcgt ataatcccgc
     2101 catttgttct tttaccgtgg tccagctgcc gactacaatc accgccggag cttttttcct
     2161 cctgtcatag gactttggat aatagatatc actcttgagt gtggaacctt cactggaaaa
     2221 agagactctt cccttgtcct gcgtcacatt tttcgcacaa tgcagaaaaa atgtcatgct
     2281 catgccgaga atacttacta ccaagaaaac tcgcgtttga tatttctttg cacccattgt
     2341 ctacctctcg atcaaaaatt tatttaatat caatacatgt tctttcataa ctttgcccat
     2401 gctctctaac caattatact agtgaggata gaggagcagg ccgatcaccc tgctagccag
     2461 tattccagaa atcacgaaga gcaatcagga gaaggcaaaa aatcctggtg aatcgctgac
     2521 ctggcgcatc gatagtgcac caccggtcat gcgcctgcat cgctctagtg aaaagctact
     2581 ataacgcctg atgatttgtc ttggaaatag ttgctgcgaa cttgtatatt attgccgttg
     2641 aacgggggtt gggctattca gaggacttgt aggcgcctgg tggatatcca aagtgctttt
     2701 tgaaggcgtt ggcgaagtga ctttgatcgc taaagccgag cttgtaggca acgtcgatga
     2761 tctggatgcc cggttccctc agcaactcct tcgatcgttc aagcttgacc ttcagaatgt
     2821 actggtaggg ggtaatcccg gtgtgcttct tgaattcctt gaggaaatag aatttgctca
     2881 gacgacagat ctcggcgagg tcttcgatgg cgatggttct ctcgatgttt tccctgatga
     2941 acctcgtgat actcccgatg tcgccgtcgt gtatcctcgg cgacgggctc ttcttcgaaa
     3001 cgaggtctgc ataattggaa tagttcctga tataatacgt ggagaaaagt ttgatcaggc
     3061 cctgcaggta ctggataccg ttcctgcccc tgatggacgc ctcattgcag aacagcctga
     3121 caaagtattc gaggctcggg tcggcgacat tgtaattctg caggaactgg agctcctgtt
     3181 ccgggagcat cccctcgaaa tgggccaaca gggttttctc atcgattgtg aatattatga
     3241 aatggcacgg atcgttgatg atgtgggtga aggggatgtt gggaggattc atccatatct
     3301 cccctggctc ggtcttcagg tcgatcagct cgccccggga ttcggctgac catttgaatc
     3361 tctcctctat cgcgagtgcg aaatagaaat acggggtgat gatattgctc gggaagaagt
     3421 gcggcgacca ccctttttcg agaagaatcc cgccccaccc gaggtcccgc gaggaaagct
     3481 cgatttccag cgggtcgccg cacgaggaac ggctgccggt ggattgttcg acgaattgaa
     3541 ggatcgtttt catgtgtcag cgcgtttctg gcagttcacg gaacaacact caatgacgca
     3601 tctatcatga ataccagatg ctgtccatat accaggacgt ctctcgccgg cggagggttc
     3661 gaacgctcat catttgcacc actccgaagc ccatttctgg atctcgtcca gcaccggctt
     3721 gaatgccttc cctttctcgg tcagctcata ttctatcctg accggaatct cgtcgtagac
     3781 cttccgcgag acgacgttca tggcctcaag ttctttcagt cgctcgatga gcatacggtc
     3841 gctcatgttg cagatcgtcc cggacaggtc gccaaaccgc ttgggcccat cagacaacac
     3901 ccgcaatatg agtcccgtcc acttcttccc cagtatgtca aatgcggctt caaacagggg
     3961 acaaacgttg ttctgtttta ccgggttttt cattttttgt aaaaaaagtc attgacacat
     4021 tgcttactaa aagtaagttg ctaattatta tatagttaat aaacaatatc aagtatctaa
     4081 taaaagttca cactttgcga tttattcacc caatgtgaaa ttaatcaatt aagtttttaa
     4141 aactttgatg gaggccatat gaaaattcta gcactggatc atgttcgtcc cggcgtaacc
     4201 atggacgaca tcgcccccca cctcaaagat gaggcccggc acgcgtggca gaattacggg
     4261 aacgggcttt tcagagagct ctactttcga ggtgaccgac cgggcgccgt tgtcatcatg
     4321 gagtgtaccg atctcgacga ggcgcacgca ataatgaaga aacttcctct cgtcagaaag
     4381 ggactcattg agttaacctt catccccctt ggcccctttg tgccctttgc atctttgttc
     4441 gaaccatcac ataattaata tcaggagaaa accatgaaac tgctgttcat caaagcgaca
     4501 cctaaaagca caatcgattc ctataccatg aaggctgcat atgcctttct cgaatcgtat
     4561 aaaaaggcca atgcggtcca tgatgtcacc gtcgtggacc tgtaccggga aaaaatggag
     4621 aatctcagcc gggacatcct gagcgagcta tattacacag ccgactcccc cctccagcgc
     4681 tacgcccggc agttcgccga agccgaccgg tacgtcttcg cggccccgat gtggacgctc
     4741 tcaatccccg gcatactgaa aacatacctt gaatacatca ccctccccgg catcaccttc
     4801 gcgaataccg agaagggacc gatgggactc ctgggcggaa aagggaaaaa ggccatccat
     4861 atcaccgcgc ggggaggaat ctattcgacg ggacccgccg cagagttcga gatgggcgac
     4921 cgttacctcc gtaccgttct ccacttcctc ggcatcgagt gtgtagacac actggcaatc
     4981 gagggaacga actttttcaa gggcaccgat ctcgacgtca ggatcaacga gtcattatca
     5041 gcggcggacc ggattggcaa aagcttctaa ccggtgacta ccgcaaaagg tattgcagcg
     5101 tatcccgtat catgacacga catacatctc ccggaggaaa aaaatgatat cgatcatcaa
     5161 cacacgaaga agcgtgagaa aattcacgtc cgacaccgta ccggaaggcg atattcggga
     5221 aatcctcgaa gccgccatga acgcgccgtc ggcgatgaac gagcaggcat ggcagttcat
     5281 cgtcctgtcg ggcgccgttc tcgatcagtt cctggcaata aacagaaaca cgccaaaagg
     5341 cgctcccatc ggcatcctgg tctgcggaga ccggcgcacg tcccgcgacg attactacgt
     5401 ccaggattgc tccgccgcca cgcagaacat actgctcgcg gtgcacgcca agggtctcgg
     5461 atccgtgtgg accaccgtgt tccccgatgc ccgtaaacca gtcgcacagt tgctgcagct
     5521 tcccgatcac gtggttcctt tttcattcac ccccatcggg cttcccgcga cagttccaaa
     5581 cgcgaaaagc agatacgacg gaggcaagat ccactataac aaatggtgat ggattaccaa
     5641 tcgatggaac aggggataca tgagaagcaa cagaattttt gtggctaagg gaaaccggtc
     5701 aggccgggag tcgggcagtg acgctgctcc aggaaatgaa ggctaatctt gtggcgggag
     5761 tgacggatgc cggcacggag atacatggcg taaaaacctt tgtcacatgc aatccgcatc
     5821 attcgatcag catactggct ccagatgatc ctgatgaaat cgtcgatgat ccgcctcagg
     5881 gccttcatgt cggtgtcgtc ccggtcgttc acatgcatga tgaaatcgtg agcgtaggga
     5941 atcgcgagaa tgcctgcacc tcccgtgtcg tcgacatcat cacaacgcga aaacgagaag
     6001 aagagcacgc cggttgcgcc gctccacacc atcccgtagt cgggaacgat aaccgcaagc
     6061 agcggctgcg cacggaccga cgatggtgtt ccgccgcaga gatgtatcgt cgaaaaaaac
     6121 atctccttta ccggcgggcc atcgatccgt ccccgtgggt gcacctcgtt gcggtcgcag
     6181 ccgtcgaagc agcatgtatc ggtgccggcg tccgtccatt cgttccccat caggacagag
     6241 tcggagcgca cagcatttct ctgcgtcttc acgattcgat agcgcccgtc gggcaggcgc
     6301 gcgaacaggg aacaatccgt gcacgatgag tcacggagcc agtcatcagc cccgttttcg
     6361 aaggcaaccc tttccctccg gatgccgatc cctccaggga tggtctctac gaacaatcgc
     6421 tcctccggat acgatcgctc gatccggtac agcggcgcgt cgtcgaagcc gagggtacat
     6481 tcgtccgaag agcgcgcgtg cagcccgaag ccccgcggag caccctgtat ttcgagcaac
     6541 gccgttctcg tatcctcagg ctgtatgcgt accatgtgga ggtggctcac cagttccctc
     6601 cgggcacact acacgacaaa gggtgtacat tcgcgtctat cgtccatgtg atcgattttt
     6661 ttcgctgcaa tccgcatcca cgatcgtctg acgagatata tccgatttgc aaaaaagtca
     6721 agcgagcgac gtttgcacgc ttgacttttt cccgcacaac ggctatcatc tctggccaac
     6781 ggcacaccag tgcatgggcg ctgatcgcac agcggcgact gcgtcggtac tcacctcatc
     6841 acgtccagga gcacgacatg aacaagactt cccgcggttt gatcgtaaca cttctgtttg
     6901 gcatctccct attctatgcg gcatcatttt caaccgacgc ggcccagccc aacggcaccc
     6961 tacacaacgc cgggcgcgtg gacgtcctgc acctgcgcgg atccttccgc gagatggggc
     7021 ggcagtatgg aacgctcgag aagaccaagc tgcgcgattt ccacgccgac atgaagaaac
     7081 tgttcgtcga caagcgcggg atcgcccccg cacggctgaa cggcatcgcc agcgagctgt
     7141 acgactccta cccgcagcgc ttccgggaga tcctcgtcgg gatggcggaa acgtccgggt
     7201 tgtcgctcga cgatcatatc ttcatcaacg ccttcgagca cttcctgttc catccggacc
     7261 tctcgcgctc ctccggcggc gcctgctccg cgatcgcggc ctggcggccc cataccgccg
     7321 acggcaatct catcttcggc cgaaactacg atttcggcga cgatgtcggc gcgtttaaac
     7381 aatacgtgaa cgtcgcgatt ttcgatcccg ccgggtccgg cgtcgccacg gcgctcgtca
     7441 ccttcgcggg gaccctgaac gcgaccacgc agctcaacgc ggcgggactc ttcctcgagc
     7501 tgaacaacgg cggcatgtcc gccggcggtc tttcccacac gaaccgcgta tcgacgccca
     7561 tcgagctctt cgccatgatg ctggactcgt cctcgctgcg gcagatcgac ctcgagatgc
     7621 atacgacgaa cacccggttt tcatacatca tcaacgtagc ggacgcgaac aatgcggtct
     7681 cgtacgagtg ggcccccttc ggcgtgaaac ggatcgaggg cgcctccggc cttctggtct
     7741 cgacgaacca tttcgtgggc gagtggggcc tggcgccatc caccgacgcc ttcttcctca
     7801 cggtgaccag gcgcaccaac ctgctgcgcc tggcggatcg cgcgaagggc gcgcttgacc
     7861 tcgcggccat gaagggaatt ctcaacaccg acatccgcgg cgggggcgcg acctcctacc
     7921 gggtcgagtc gatcggcggc aacccggccc cgtacaccgc ctaccaggtc atcgccgagc
     7981 cggcgcggcg gcgcgtctgg atcaggctgc ccggctatca ggactggatc gcgcttgagc
     8041 tgggaccgct gtttgggaag cgatagcgca tgatcccgca gggacatcgg ccccccatcg
     8101 cgacgccggg atctcccgcc gcgcatgccg tcgattctgc accgggagcg gcagtgcggc
     8161 gtcaccgcgc gcggaatcat attgcgcaca acggaaacga gcgcacacac acgaccttcg
     8221 ccggcaggag ctcaatgata tgatccatca ccccagcacc gtccattggg acaccacgct
     8281 caagcagatc ttcgacgaga tcgacgacta tttggagcac acatacgggg acttgtacca
     8341 tcttcgcccc aatcgacccg cgcgcggcga gacgagcaac aaggagcacg acggcctgtt
     8401 caatgtggga tcgagctttt cggcggggtt cggcagcagc ctggggcgcg gcttcgtcgt
     8461 gtcagtccac ctctccaccc tgcaaccggt cgagagcgcc gtctatcatc aaatcatgga
     8521 ggtggtcatc cggctcctgc gcgaaaagct gccggaacgc tttcccggcc gtgagctcaa
     8581 ggtcgactgg gacggcgaca acctcaagat ccacggcgac ctcacgctgg gcgagctgta
     8641 accccacggt ccggcgctca ccgcggcgtc gatccgtact cgcacaaact ccgcacgggg
     8701 cattccgcac aacgcggccg ccgcgcatcg cagagggaac ggccatgcgt gatgagaacg
     8761 aggtgcgccg ccgtccagtc gcgctccggg acgagcgcgg tgacggccgc ctccaccgcg
     8821 tccggatcgt ccgactcgat gaagccgatg cggttcgcga tgcgcaggat atgcgtgtcc
     8881 accgcgaagg ccggcacgcc gaagccgatg gacaggatca cgttcgcgga ctttcgcccg
     8941 atcccgggaa ggcgcatgag cgcctcgcgc gttccgggca acgcgccgcc gaactcgtcc
     9001 acgaccgcgc gcgccatccc cacgatgttc ttcgccttgt tccggaaaaa tcccgtgctc
     9061 ttgacgatgc gctccacatc actgatgcgc gcccgggaga gccgttcgaa atcgggatag
     9121 cgctcgaaga ggatcggcgt gacctcgttc acctggcggt cggtggtttg cgcggagagg
     9181 gagacggcga cggcgagctg atagcggtcc ccgaaggcga gggccggcgc gggcgttccg
     9241 tagtgttcga ggaggcggga gatgacggcg cgcgcccgcc gcgcggtcat cgccgcaccg
     9301 cgccgggccg ccgcgctcat c
//
LOCUS       DAORHF010000144         9307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6820_length_9307_cov_4.580523, whole genome shotgun sequence.
ACCESSION   DAORHF010000144 DAORHF010000000
VERSION     DAORHF010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6820_length_9307_cov_4.580523"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PKJ16_12020"
     CDS             <1..408
                     /locus_tag="PKJ16_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV47759.1"
                     /translation="RVMNRVTTGFIDEAVWEYIGNQCFECGACSFVCPTCSCFNIVDL
                     PRKNGGAHESDRVRTWDSCSYEGYTREAGGHNPRKPVEDRRNKRFFCKLSYSQSKKYL
                     RPGCVGCGRCQWVCPGDIGLPNVVTYIRREIHS"
     gene            421..1248
                     /locus_tag="PKJ16_12025"
     CDS             421..1248
                     /locus_tag="PKJ16_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HNV47760.1"
                     /translation="MENGKIVTIPAVATIDEIKDEIPNVRTYYLSFDDKAIDGAFTFR
                     SGQFIMLTVFGEGECAISLPPSPEKDRFHITVRSVGKVTRALGGLSAGDKVGVRGPFG
                     NGFPFDEIKGKNVVFVAGGIGLIPLRSSIVHVLQHRKDFGRVILLSGSRTPSDIMYKY
                     NMKEWQENCEAFYTVDNPTPDWTGETGFVHTLIEKAKVPVENTVAFVCGPPVMFNTVN
                     QELMKRGLSDEFIISTLERHMKCGVGKCQHCAVGRTLVCTDGPVYTYRQIKILGEQI"
     gene            1248..1754
                     /locus_tag="PKJ16_12030"
     CDS             1248..1754
                     /locus_tag="PKJ16_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HNV47761.1"
                     /translation="MELSHTLHAILADTTCIVGIGNYLRSDDAVGLEIVDELKDCARS
                     ERVSVINAEDVMESFLGDIRGTGARNVIIIDAVSSVSEPGNVVFGRLEDFDELIGGYS
                     THKLALKLACQTLADAGQRVYLLGIGIMTNEIGVGLSANVRRTADTLAGILAHIIKSS
                     HKEYVYVQ"
     gene            1744..3615
                     /locus_tag="PKJ16_12035"
     CDS             1744..3615
                     /locus_tag="PKJ16_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HNV47762.1"
                     /translation="MFSDLYGNLLVAFALLIISAVVAPFLATKRTLAGWLNFLMVAAA
                     SVIMLGIGYETVFGGNSLPSKLIAFGPVKIYFLLDSFAGFFAGVIGFMAIMSAFYSIA
                     YMEHYPDYRLRSYYANFPIFIAGMFGIVTVDDLSVGFTIAWQMMTIASFFLVRFEYKK
                     AENVRNANKYLVLMEIAWALILAGTVFISNFGIGDSVHELAAKLGATTGAAPYIVYAL
                     IFLGFAFKAGVFPLGQLWLPDAHSIAPSPISALLSGVMIKTGIYGIIRTFFWMVPHTE
                     TFHFDGTLWGAIIASIGVVTLFMGTAQSMKQSDAKRLLAYSSIGQIGYITMAVGAALF
                     MFSSNNEFLKLVSLVAIIGAVYHILNHAVFKGLLFLSSGSVLYATGTKDLNRLGGLIT
                     IMPVTAVIAGVASLSISGVPGFSGFASKWTIVSSSLLAGSDVLFLVIFGIVALFTSAL
                     TLSCYVKFFGMTFTSTGTEWTVQKDIKEVPAIMLAPKIVLTALCLVQGLIPFFYYQAI
                     IGIMQSAKGSIMFEAFRSVDFDNFILSTAMGVSVGVPGIKGLTLAAAFPVIVLAVFAG
                     AFAIAALLRRSGGSEEKLTTTWLCGYQELSNNNRYKDKNMFAALKNVLRWTGGNVKD"
     gene            3642..5327
                     /locus_tag="PKJ16_12040"
     CDS             3642..5327
                     /locus_tag="PKJ16_12040"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HNV47763.1"
                     /translation="MPSRDDIKKSLTDSFKGAIVKMEQPIDSRLFVDIHKEKVREISN
                     AIVKMGGRYQVSIGYDNIARDKTLGLVHAFAFDRENLFLLLRTSAPADAPVFDSITPD
                     IPNAGWSEREYQDLLGMKFTGHPKPKRLILADDWPAGVYPLRKEIPWNLMPPAAEDVA
                     YQLDEAPAGCTTVPVGPFHPTLHEPAHFAVFVDGETIKGCDYRGFMTHRGIEKLCQTQ
                     VSYNEIPFIAERICGICGSVHATAYSQAAEMAFGMKISRRAEYIRTIMLEIERIHSHL
                     LWLGVAGHLIGFDTVFMQAWRVREPVMWLGERITGNRKTYGMIVIGGVRRDITPEVKE
                     DILNVIKKIETELMTIHKAIAGDSAIHKRTKGVGALTREQVIDWSLVGPVARARNVDI
                     DARRDLPYAAYDDMKFSVPVVDTMDVWGTVVVRILETFEAISIIRQALDKMPNDGPIL
                     TPVTEEIPPLAHAISSIEAPRGESVHYIITGENENRPERWRVRAPTYPNLQGVPAMMM
                     NNQLADVPIIIGSIDPCFSCTDRMMLVDMQSGKAKEVSGKELEELSRKLTTGR"
     gene            5331..6230
                     /locus_tag="PKJ16_12045"
     CDS             5331..6230
                     /locus_tag="PKJ16_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HNV47764.1"
                     /translation="MEIAAIIINVTLFLALAPLFEGVIRKITARVQSRQGPPVYQPYL
                     DLLKLLGKQNLVKGNWAFSAAPAIALASIITAIAFVPLGYKATFLTGYADVITVIYLL
                     TLGGVAVLIGALASKNTYAVIGASREMITIIMVEPVLAMTFIMGAVKVKSLGLDASVF
                     SIAVSGYSWSVAVMLAAFLLALQAFVARQPFDIAEAEVEILEGPFIEYSGPNYALFKY
                     YMMLKQMFYAALFVTMFVPFLRTGYYFADLGIQLAEIFGVFVLISLIGSTNPRFRIDQ
                     AVRYYAALIVLSLAAVGLSVYGL"
     gene            6280..6822
                     /locus_tag="PKJ16_12050"
     CDS             6280..6822
                     /locus_tag="PKJ16_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV47765.1"
                     /translation="MTVWLTSKIKQVLMVLKPGTVTLGYPFIPNPAPKDFRGQPYWDH
                     HKCIGCGGCAAHCPARTILVRDLCQELRVMLYDSSRCTYCGRCADVCPESAITMTDKF
                     ELATGDREDLTERMELFMLTCQRCGRCYNMETENIIDKMDLKGYRYDNLDARALIPKA
                     TECIDTAVLTETETYQRPAK"
     gene            6834..7364
                     /locus_tag="PKJ16_12055"
     CDS             6834..7364
                     /locus_tag="PKJ16_12055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018749468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HNV47766.1"
                     /translation="MLARLAKKAFPKSLWVYHCNSGACNGCDIEILNVLTPYYDVERF
                     GIKLVASPRHADVMLISGAVTRPTLPLVKRAYEAIPEPKLVFGIGSCATGGGCWFDSY
                     NVCGGAHTAVPVNYFIPGCPPRPEAIIYGVALALGLVDKKVAPIELKQMEFPIDSIAR
                     NKAWEERNVIYQCLEK"
     gene            7410..7811
                     /locus_tag="PKJ16_12060"
     CDS             7410..7811
                     /locus_tag="PKJ16_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47767.1"
                     /translation="MAKKILLVDDDTDLVASLGQVLRGNGYTVVEAYSGAEGLKKLLA
                     ERPDLMILDVMMETDTAGFEITYQIRDTRDSSRYAEVRTVPIIMLTAINQVTNSRFSL
                     NEKESFLPGVNDFLTKPVKIEDLLEKVKRYA"
     gene            7906..>9307
                     /locus_tag="PKJ16_12065"
     CDS             7906..>9307
                     /locus_tag="PKJ16_12065"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HNV47768.1"
                     /translation="MPLRRVKANIRIKLTASLLSVVLITGGVSVVVGVSIINDKVVGQ
                     AYETVQNDLKTARYIYDDMINVIHLFIKHLASLAYLQEAVAGNDRALLIRKLKEVREE
                     LNLDVLNITDATGRVIVRARNENVIGDSVADDSFVREAIRNRASPRGTGIMGREELLR
                     EGRDLAEAAYIEIRPTPMARAKEAAVEERAMVLKAAAPIVRQGRLIGVIYGAKVLNKD
                     YTFVDRIKRMVFKDDTFKGTPIGTATVFLNDLRISTNVIRKDGSRAIGTRVSEDVYNK
                     VIVNQGLWLDKAFVVNDWYISAYSPIYDIENRVIGILYVGILEAKYQKIRNDAAFYFL
                     LIIIISAIIAILLSLYLIQNIIIPINALVDASRDIANGIYTKKLEVESADELGYLCLT
                     FNKMIDAIEERDRRLKEQTEQKLAQSEKLASLGRLASGIAHEINNPLTGVLTYSSLLL
                     EDLKGTDHEDDLHVIVT"
BASE COUNT         1808 a         3026 c         2785 g         1688 t
ORIGIN      
        1 cgcgtgatga accgcgtgac cacgggcttc atcgacgagg cggtgtggga atacatcggc
       61 aatcagtgct tcgagtgcgg cgcctgctcg ttcgtgtgcc ccacctgctc gtgcttcaac
      121 atcgtcgatc tcccgcggaa aaacggcggg gcgcacgagt cggaccgcgt ccggacatgg
      181 gactcctgct cgtacgaggg atacacccgg gaggcgggcg gccacaatcc gcgcaagccc
      241 gttgaggacc ggcgcaacaa gcgcttcttc tgcaagctct cgtactcgca atcgaaaaaa
      301 tacctgcggc ccggctgcgt gggctgcggt cgctgccagt gggtgtgccc cggcgacatc
      361 gggctgccca acgtggtcac gtacatcaga cgggaaatcc acagctaggt gacggtgatg
      421 atggaaaacg gcaaaatcgt aacaataccg gcggtagcca ccattgacga gatcaaggac
      481 gagatcccca atgttcggac ctactatctc tcctttgacg acaaggcgat cgacggcgcc
      541 ttcacgttcc gctccgggca gttcatcatg ctcacggtct tcggcgaggg cgagtgcgcc
      601 atctcgctgc cgcccagccc cgagaaggac cgcttccaca tcaccgtgcg cagcgtgggc
      661 aaggtgaccc gcgccctcgg gggcctgagc gccggggaca aggtcggggt gcgcggcccc
      721 ttcggcaacg gcttcccctt cgacgagatc aagggcaaga acgtggtctt cgtggccggc
      781 ggcatcggcc tcatcccgct gcgctcgtcg atcgtgcacg tgctgcagca ccggaaggat
      841 ttcggcaggg tgatcctgct ctccggctcc cgcaccccca gcgacatcat gtacaagtac
      901 aacatgaagg aatggcagga gaactgcgag gccttctaca cggtggacaa tcccacgccg
      961 gactggaccg gcgaaacggg cttcgtccac accctcatcg agaaggcgaa ggtgccggtg
     1021 gagaacaccg tggccttcgt gtgcggcccg ccggtgatgt tcaacacggt gaaccaggag
     1081 ctcatgaagc gcggcctctc ggacgagttc atcatctcca cgctcgagcg gcacatgaag
     1141 tgcggcgtcg ggaagtgcca gcactgcgcc gtgggccgga cgctggtgtg caccgacggc
     1201 ccggtctaca cctaccggca gatcaagatc ctgggggagc agatataatg gaactctcgc
     1261 acaccctgca cgcaatcctc gccgacacca cctgcatcgt ggggatcggc aactatctgc
     1321 gcagcgacga cgcggtgggg ctcgagatcg tggacgagct caaggactgc gcccggagcg
     1381 aacgcgtgag cgtcatcaac gccgaggacg tgatggaaag cttcctgggc gatatccgcg
     1441 gcacgggcgc gcgcaacgtc atcatcatcg acgccgtctc gtcggtcagc gagccgggca
     1501 atgtcgtgtt cggccgcctc gaagatttcg acgagctcat cggcgggtac tcgacccaca
     1561 agctcgcgct caagctcgcc tgccagacgc tcgcggacgc cggacagcgc gtgtacctcc
     1621 tcggcatcgg catcatgacc aatgaaatcg gcgtggggct ttccgcgaac gtccggcgca
     1681 cggccgacac gctcgccggc atcctcgcgc acatcatcaa gagcagccac aaggagtatg
     1741 tctatgttca gtgatctcta tggaaacctg ttagtcgcct tcgccctgct gatcatatcc
     1801 gccgtcgtgg cgcccttcct cgcgacgaag cggacgctcg ccggctggtt aaatttcctc
     1861 atggtcgcgg ctgcctccgt catcatgctg ggcatcggct acgagaccgt cttcggcggg
     1921 aactcgctcc cgtccaagct catcgccttc gggccggtca agatctactt cctgctcgac
     1981 agctttgcgg gcttcttcgc cggcgtcatc ggcttcatgg ccatcatgag cgccttctat
     2041 tcgatcgcct acatggagca ctacccggac taccggctgc ggagttatta tgccaacttc
     2101 cccatcttca tcgcgggaat gttcgggatc gtgaccgtgg acgacctctc ggtgggcttc
     2161 accatcgcct ggcagatgat gaccatcgca tcgttcttcc tggtgcgctt cgaatacaag
     2221 aaggcggaaa acgtccgcaa tgcgaacaag tacctcgtcc tgatggagat cgcctgggcg
     2281 ctcatcctcg ccggcaccgt cttcatcagc aatttcggca tcggcgattc cgtgcacgag
     2341 cttgccgcaa agctcggcgc gacgaccggc gcggcgccct atatcgtcta cgcactcatc
     2401 tttctcggct tcgccttcaa ggccggcgtg tttccgctcg gccagctctg gcttcctgac
     2461 gcacattcga ttgcgccgtc cccgatcagc gccctcctct cgggggtgat gatcaagacc
     2521 ggcatctacg gcattatccg tacgttcttc tggatggtgc cgcacacgga gaccttccat
     2581 ttcgacggga ccctgtgggg cgccatcatc gcttcaattg gtgttgtgac cctctttatg
     2641 gggacggcgc aatcgatgaa gcaaagcgac gccaagcggc tgctcgccta cagctcgatc
     2701 ggccagatcg gctacatcac catggccgtg ggcgcggcgc tgttcatgtt ctcctcgaac
     2761 aacgagttct taaagctcgt ctcgctcgtc gcgatcatcg gcgcggtgta ccacatcctc
     2821 aaccacgcgg tgttcaaggg cctgctcttt ctctcgagcg ggagcgtcct gtacgcgacc
     2881 ggcaccaagg acttaaaccg gctcggcggt ctcatcacca tcatgccggt cacggccgtg
     2941 atcgccggcg tcgcgtcgct ctcgatctcc ggcgtccccg gcttcagcgg cttcgcgagc
     3001 aagtggacca tcgtgtcctc gtcgctcttg gcgggaagcg atgtgctctt tcttgtgatc
     3061 ttcgggatcg tcgcgctgtt cacgagcgcg ctcacgctgt cgtgctacgt gaagttcttc
     3121 ggcatgacct tcacctcgac gggcaccgaa tggaccgtcc agaaggacat caaggaggtc
     3181 ccggccatca tgctcgcgcc caagatcgtc ctcaccgcgc tgtgcctggt gcaggggctc
     3241 attcccttct tctactatca ggcgatcatc gggatcatgc agagcgcgaa gggctccatc
     3301 atgttcgagg ccttccgaag cgtcgacttc gacaatttca tcctctccac cgccatgggc
     3361 gtgagcgtgg gcgtgcccgg catcaagggg ctcacgctgg ccgcggcctt tccggtgatc
     3421 gtcctggcgg tcttcgcggg cgccttcgcc atcgccgcgc tgctccgccg ctcgggcggc
     3481 tcggaggaga agctcacgac cacgtggctc tgcggttacc aggagctatc gaacaacaat
     3541 cgctacaagg acaagaacat gttcgccgcg ctcaagaacg tcctgcgctg gacgggcggc
     3601 aatgtgaagg actgatcgaa ttcatcacaa cgaggtgaac tatgccttcg agagacgaca
     3661 tcaaaaagag cttaaccgat tccttcaaag gcgccatcgt gaagatggag cagccgatcg
     3721 acagcaggct ctttgtcgat atacacaagg agaaggtgcg cgagatctcc aacgccatcg
     3781 tgaagatggg cggccgctac caggtgagca tcggctacga caacatcgcg cgcgacaaga
     3841 ccctcgggct ggtgcacgcc ttcgcgtttg atcgcgagaa cctcttcctg ctgctgcgga
     3901 cgtcggcccc ggccgatgcg ccggtgttcg attccatcac cccggacatc cccaacgccg
     3961 gctggtccga gcgcgagtac caggacctcc tggggatgaa gttcacgggg caccccaagc
     4021 ccaagcggct catcctcgcc gacgactggc cggccggggt gtacccgctg cgcaaggaga
     4081 tcccctggaa cctgatgccg ccggccgccg aggacgtggc ctatcagctc gacgaggcgc
     4141 cagcgggctg caccacggtg ccggtgggcc cgttccatcc gacgctccac gagccggcgc
     4201 acttcgcggt gttcgttgac ggcgagacca tcaagggctg cgactaccgc ggcttcatga
     4261 cgcatcgcgg catcgagaag ctgtgccaaa cgcaggtgag ctacaacgag atccccttca
     4321 tcgccgagcg gatctgcggg atctgcggct cggtgcacgc gaccgcgtac tcgcaggccg
     4381 ccgagatggc cttcgggatg aagatctcgc gccgcgccga gtatatccgc accatcatgc
     4441 tggagatcga gcgcatccac tcgcacctcc tgtggctcgg cgtcgcgggg cacctcatcg
     4501 gcttcgacac cgtgttcatg caggcctggc gcgtccgcga gcccgtcatg tggctcggcg
     4561 agcgcatcac cggcaatcgc aagacctacg gcatgatcgt gatcggcggc gtccggcgcg
     4621 acatcacccc cgaagtaaaa gaagacatac tgaacgtgat caagaagatc gagaccgagc
     4681 tcatgacgat ccacaaggcc atcgcgggcg actccgccat tcacaagcgc accaagggcg
     4741 tgggcgcgct cacccgcgaa caggtcatcg actggagcct cgtggggccc gtggcccgcg
     4801 cgcgcaacgt cgatatcgac gcgcgccgcg acctccccta cgccgcctac gacgacatga
     4861 agttctccgt gccggtcgtg gacacgatgg acgtgtgggg cacggtggtg gtccgcatcc
     4921 tcgagacctt cgaggcgatc agcatcatcc ggcaggcgct ggacaagatg cccaacgacg
     4981 gccccatcct cacgccggtt accgaggaga ttccgccgct cgcgcacgcg atctcgtcga
     5041 tcgaagcacc gcgcggcgag tccgtccact atatcatcac cggtgagaac gagaaccggc
     5101 ccgagcgctg gcgcgtgcgc gcccccacat acccgaacct ccagggcgtc ccggcaatga
     5161 tgatgaacaa ccagctggcc gacgtgccca tcatcatcgg cagcatcgac ccgtgtttct
     5221 cctgcaccga ccgcatgatg ctggtggaca tgcaatccgg gaaggcgaaa gaggtgtcgg
     5281 gcaaggagct cgaggagcta tctcgcaaac taacgacagg caggtaagcc atggaaatcg
     5341 cagccatcat catcaacgtg actctgttcc tggcgctcgc gccgctgttc gagggcgtga
     5401 tccgcaagat caccgcgcgc gtccagtcgc ggcaggggcc gccggtctat cagccctacc
     5461 tggacctgct caagctcctg ggcaagcaga acctcgtgaa gggcaactgg gccttctccg
     5521 cggccccggc gatcgcgctg gcgtccatca tcacggccat cgccttcgtt ccgctgggct
     5581 acaaggcgac gtttctcacg ggctatgccg acgtgatcac cgtcatctac ctgcttacgc
     5641 tgggcggggt ggcggtcctc atcggggcgc tcgcgagcaa gaacacctac gcggtgatcg
     5701 gcgcgagccg cgagatgatc accatcatca tggtggagcc ggtgctggcc atgaccttca
     5761 tcatgggcgc cgtcaaggtg aaaagcctcg gcctggacgc ctccgttttc agcatcgccg
     5821 tgagcggcta ctcctggtcg gtggccgtca tgctggccgc cttcctgctc gcgctgcagg
     5881 ccttcgtggc gcgccagcca ttcgacatcg ccgaggccga ggtggagatc ctggagggcc
     5941 ccttcatcga gtacagcggg cccaactacg cgctcttcaa gtactacatg atgctcaagc
     6001 agatgttcta cgcggcgctg ttcgtgacga tgttcgtacc gtttctgcgg accggttatt
     6061 atttcgcgga cctgggcata cagctcgcgg aaatcttcgg ggtgttcgtg ctgatatcgc
     6121 tcatcggctc gaccaaccca cgcttccgga tcgaccaggc ggtgagatac tacgccgcgc
     6181 tcatcgtgct gtcgctggcg gcggtcgggc tgtccgtgta cggcctgtag gggtgatgac
     6241 ctcatcccga aaggggaaac tattgatgaa taaaggagaa tgaccgtgtg gctgacaagt
     6301 aaaatcaaac aggttctcat ggttctcaag cccggcacag tgacgcttgg ctacccgttc
     6361 atccccaacc cggcccccaa ggattttcgc gggcagccct attgggacca ccacaagtgc
     6421 atcggctgcg gcggctgcgc ggcgcactgc cccgcccgca cgattctggt gcgcgacctc
     6481 tgccaggagc tgcgcgtgat gctgtacgac tcatcgcgct gcacttactg cggacggtgc
     6541 gcggacgttt gtcccgagag cgccatcacc atgaccgaca aattcgagct cgccacgggg
     6601 gaccgcgagg acctcaccga acgcatggag ctgttcatgc tcacctgcca gcgctgcggg
     6661 cgctgctata acatggaaac cgagaacatc atcgacaaga tggacctgaa gggctaccgc
     6721 tacgacaacc tcgacgcccg cgcgctcatc ccgaaggcga ccgagtgcat cgacacggcc
     6781 gtcctcacag agacggaaac ctatcagaga ccagcgaaat gaggagatga accatgctag
     6841 cacgactcgc gaaaaaggcc tttccaaaat cactctgggt gtatcactgc aactcggggg
     6901 cctgcaacgg ctgcgacatc gagatcctga acgtgctcac tccgtactac gacgtggagc
     6961 gcttcggcat caagctggtg gcctccccgc gccatgccga cgtgatgctc atctccggcg
     7021 ccgtgacgcg gcccacgctc ccgctggtga agcgcgccta tgaggcgatc ccggaaccca
     7081 agctggtctt cggcatcggc tcctgcgcga ccggcggcgg ctgctggttc gattcgtaca
     7141 acgtctgcgg cggcgcgcat acggccgtcc cggtgaacta cttcatcccg ggctgcccgc
     7201 cccgccccga ggcgatcatc tacggggtcg cgctggcgct tgggctggtg gacaagaagg
     7261 tcgcgcccat cgagctcaag caaatggagt tcccgatcga ctccatcgcg cgcaacaagg
     7321 cgtgggagga gcgcaacgtg atttaccaat gtctggagaa gtaacgggaa cggcgtgaga
     7381 gggacaacta ccctgatttg gagataacca tggcgaagaa aatcttgctc gtagatgacg
     7441 acaccgatct ggtggccagc ctgggacagg tgctccgggg aaacggctat acggtggtcg
     7501 aggcctatag cggggcggaa gggctcaaaa agctcctcgc cgagcggccc gacctcatga
     7561 tcctggacgt catgatggaa accgacaccg ccggcttcga gatcacctat cagatccgcg
     7621 acacccgcga ctcctcgcgc tacgcggagg tgcgcaccgt tcccatcatc atgctcaccg
     7681 cgatcaacca ggtgaccaac tcccgcttct ccctcaacga gaaggagagt ttccttccgg
     7741 gcgtgaacga tttcctcacc aagccggtga agatcgagga cctgctcgag aaggtgaaac
     7801 gatacgcgtg agcgcgtacc cggctcgtga gcgccgggat catgttttat cggaggggaa
     7861 gagattttag ttgatttctt ccttttcccg ttataggggt gcgggatgcc gctacgtcga
     7921 gtcaaggcca acattcgcat taaactcacc gcaagcctgc tgtcggtggt gctcatcacc
     7981 ggcggcgtgt cggtggtggt cggcgtgtcc atcatcaacg acaaggtggt gggccaggcc
     8041 tacgaaacgg tccagaacga cctgaagacc gcccgctata tctacgacga catgatcaac
     8101 gtgatccacc tgttcatcaa gcacctcgcg tcgctggcgt atctccagga ggccgtggcc
     8161 ggaaacgacc gcgccctcct gattcgaaag ctcaaggagg tccgtgagga gctcaatctc
     8221 gacgtcctga acatcaccga cgcgacgggc cgggtgatcg tccgcgcccg aaacgagaac
     8281 gtcatcggcg actccgtggc cgacgacagc ttcgtccgcg aggccatccg aaatcgcgcg
     8341 tccccccggg gcaccggcat catgggacgc gaggagctcc tgcgcgaggg gagagatctt
     8401 gccgaggccg cctacatcga gatccgaccg acgccgatgg cccgcgcgaa ggaggccgcc
     8461 gtcgaggagc gcgccatggt gctcaaggcc gccgcgccca tcgtccgtca gggccgcctc
     8521 atcggcgtca tctacggcgc gaaggtgctc aacaaggact acaccttcgt ggaccgcatc
     8581 aagcgaatgg tgttcaagga cgacaccttc aaggggacgc ccatcggcac ggcgaccgtc
     8641 ttcctgaacg accttcgtat ttcaaccaac gtcatccgga aagacggctc gcgcgcgatc
     8701 ggcacgcgcg tgtccgagga cgtatacaac aaggtgatcg tcaatcaggg cctgtggctc
     8761 gacaaggcct tcgtcgtgaa cgactggtat atttcggcct acagccccat ttacgacatc
     8821 gagaacaggg tgatcggcat cctgtacgtc ggcatccttg aggccaagta ccagaagatc
     8881 cgcaacgacg ccgcgttcta cttcctgctg atcatcatca tttcggccat catcgcgatc
     8941 ctgctgtcgc tgtacctgat acagaacatc atcattccca taaacgccct ggtggacgcc
     9001 tcgcgcgaca tcgccaacgg gatctacacc aaaaagctgg aggtcgagag cgccgacgag
     9061 ctggggtacc tctgcctcac cttcaacaag atgatcgacg ccatcgagga gcgcgaccgg
     9121 cggctcaagg agcagaccga gcagaagctc gcccagtccg agaagctcgc gtcgctggga
     9181 cggctcgcct ccgggatcgc gcacgagatc aacaacccgc tcaccggcgt gctcacctac
     9241 tcgagcctcc tgctcgagga cctcaagggg accgatcacg aggacgacct ccacgtgatc
     9301 gtgaccg
//
LOCUS       DAORHF010000145         9208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6936_length_9208_cov_4.315525, whole genome shotgun sequence.
ACCESSION   DAORHF010000145 DAORHF010000000
VERSION     DAORHF010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9208
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6936_length_9208_cov_4.315525"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="PKJ16_12070"
     CDS             complement(<1..653)
                     /locus_tag="PKJ16_12070"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV47769.1"
                     /translation="MKKFTSGLIAVGIVLVIAGGFMFMNSAPSDLVTEIVAVKKGDTM
                     RSVAHALAERNIVRSEAFMYRIAQATGRTAVIAGRYKVHPGTTAFEIVQKINRGDIIR
                     RKVTIPEGYNIYQIAERLQQNEVVRADAFLALVADPAFLASIGINAPLAEGYLFPDTY
                     TFAEDADPRAVITMMHNRLQKVLDGLDLSPLKKHSMDLHALLTLASMVEKEARVPQER
                     "
     gene            1065..2108
                     /locus_tag="PKJ16_12075"
     CDS             1065..2108
                     /locus_tag="PKJ16_12075"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HNV47770.1"
                     /translation="MFTACTSSDDDVFTGTGTDVRVVTVAGDGTADLTDGVGTAAQFN
                     QPDGVTVNKTNGLIIVGDTGNNAIRTIAADGTVVTLAGQKTPGFADGDGAAAQFDQPD
                     GVAVDSSGNVYVADTGNNRIRKITPGGTVTTIAGDGTADFKDDNGTSAQLNGPTGITV
                     DSSGIIFVADSGNNVIRRIDSAGNVTTVAGDIVGQAAGDADSATGTDARFKKPHAVTV
                     DSSGNLYVTDHENNKIKKITPGFAVSTYAGDGTAAFLDGVASASRFNRPHGLTVDGAG
                     NIVTGDELNNRIRFITPSGVVQTYAGNGTAGYADGSAASAQFKQPRGIALDTSGNILI
                     CDYDNNRIRKIVR"
     gene            2115..2348
                     /locus_tag="PKJ16_12080"
     CDS             2115..2348
                     /locus_tag="PKJ16_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47771.1"
                     /translation="MGPLTLVYLRDGRMILTAEPFADWREAQDRYADYMSSLAFETIE
                     AVRRYLVLEYRITEERAAVITAGWQSGEVEIEV"
     gene            2699..4717
                     /locus_tag="PKJ16_12085"
     CDS             2699..4717
                     /locus_tag="PKJ16_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47772.1"
                     /translation="MKRNRQQHFIRAAWGLLFVCACIALPGDSGTVPEKNAPYYNKTG
                     WEHLKTGNAHKAILAFRNALRQNPRYKEAIVGLAQGYLSTEAYDESLKLFEDAVKYDR
                     KNSAILSGKGMALAGLGRYHEALAAFDEAVKLSEENLDAKYGLARLYYLMDKRLWAKR
                     KLETIFRSNPFHYDSLLLAAEIKAVEKRLDEATAFVKKAVESNPELPQGYVRFGDIYL
                     REYLKRYDDDFVVKAVEEFGRALALQPDHFAGNRYLGIVALIKGNMGEAAVHFRKCLA
                     VNPKSVPALYGLAVALAGSGSLDEAEKQYAAALAVAPSNGIVQNGFEDFLVGSGFAMG
                     NPSRVDLSRAHLTTARDCMKKKLPVQSIYHLRRTLLLNPLDREAREALRDYYYAQDYY
                     RFYVDEIGTLVKLYPSEKYQEALEIAVMKRRDRLYQRVGYGTEPPPRDVPSVFVLDLH
                     PRGEMPAHYDAGRVIANAISFSLAQWGRMRPVGMKDRAETLRGIRFDDEYIEKSLERI
                     NDRAGKGALGPVDYVLYGDFREGVGFVHITLNLLNRTNGVVITSFTLSELGRNNLPEV
                     ALRAARKVYEKIPFRGRVLKMDEDKPIVNLGGFDGLKKDDIFVIYKDAPRDSHAGGIG
                     KKMKILLTVGEADTLVSALSVERAQDLAMVDVGDAVYPLEKRRAKRLK"
     gene            4812..5270
                     /locus_tag="PKJ16_12090"
     CDS             4812..5270
                     /locus_tag="PKJ16_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47773.1"
                     /translation="MKRMAVYGIAVVLMAACAAGCGKGSYGGLKYGDIVPGDWLQANV
                     VDAKTMKRAMWFQIFDQKGDDSIVSYYKKSSETVAGYPANVNKDKWIWILINNRIEIR
                     LLADDEHPDFQKTEKLAEFLKRFDLTGLSKIDGAKVQGSALRKYMPKLGS"
     gene            5323..6513
                     /locus_tag="PKJ16_12095"
     CDS             5323..6513
                     /locus_tag="PKJ16_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV47774.1"
                     /translation="MKKIAITALFVIAVPLAGSAHRLEWNIPQGKRLEIVRTAEVEYR
                     KNSRVEKTYTERNIIDFTCQERTGDGSRVKGQFTVYSRGAGEEVFNLRERFDSEFTIA
                     PTGRFTVPPQFIMPNIRHVPTFPAGGVSVGAKWSAPAEVLINAFKPPLMLQLDAQYFL
                     TAVEKRGGTTVGVINYYLHIDKDLVGKVTGENYPARIFGYYFATLNWDLERNLPIDSI
                     EKYTIMFLFGSDRTGYDSLEFRMNITSQNKLYENVGKDETDRQAKDLAGKLPSGSGVT
                     VDTHEKGLVLRLGEVLFDVNSARIREDAKGNLDVIGKLLKERYPDREIIVEGHTDNTG
                     ESPYNDKLSHDRAKSVGAHLKGRIGHNKLSFIGHGPRKPIADNRTKEGRQKNRRVEII
                     IKLN"
     gene            6698..>9208
                     /locus_tag="PKJ16_12100"
     CDS             6698..>9208
                     /locus_tag="PKJ16_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47775.1"
                     /translation="MNPLPGYTIFEMIDETSRSLVHRGRRDSDGTGVVIKLLKTDRAT
                     PTDLARFRQEYAIIREIDDEGVVRTLDIIDCDGGIAIVMEDFGGVSLKRALREGKADL
                     DFVLGTGASIASALGRLHGRKIIHGDIKPGNIIISADSLTPAPPRPLPGGEGSVKIAD
                     FGIARAVTRADENINDPNVIEGTLAYMSPEQTGRMNRTIDHRTDLYSLGVTLYEMLTG
                     EVPFRSADPMEVIHAHIAKKPVPPHEAGRPVPSAISAIVMKLLAKAAEERYQSAAAVA
                     EDLGRCLEMLRSNGRIDDFAIAANDIAADFRFSYVFVGREKEIGELYAAFERVAVNIR
                     PNLPVGAGERSRMELVLVAGPPGIGKTALVNELQRPVLAERGYFISGKFEQYSRDIPY
                     GALVQAFRSLAARMLAESPERLAAWRERLKAGFGNIGKVITDIVPELALVAGNQPDVP
                     ELGPQESQNRFNYAFRNFVAAITAAGPIVLFLDDLQWADMASLNALRTVAADPDIGNI
                     LIICAYRDTEVGAAHPAMLSLDDMRKSMTITELSLPPLETDRVNDIISLLLKTCSGET
                     ASLAELVFAKTGGNPFFVYQFLGLLRDRDLLKTGSRGWTWDMDAIRSLPVTENVVDLM
                     AGKISMLPEETRKALSAAACIGTVFGLDLLAKVIDVSIDDALRLIAPAIIEGFLVAEE
                     DEYRFTHDKIQEVSYILLPENDRNALHYRIGNILLNTTSEDILDEKIIPIADQLNCGS
                     SMAHRDDEKVRLAGVNLKAARKAWRAAAFQSSYRYSGSGIVNLPPDSWVVRYDLCLEL
                     FNIGVQAAYLSDDYDGMSRLAEVVLRNARRVTDCTTVYA"
BASE COUNT         1852 a         2704 c         2956 g         1696 t
ORIGIN      
        1 cgctcctgcg gcacgcgcgc ctccttctcc accatcgagg ccagggtgag gagggcgtgc
       61 aggtccatcg aatgcttttt aagcggcgag aggtcgagcc cgtcgagcac cttctggagg
      121 cggttgtgca tcatggtgat caccgcccgc ggatcggcgt cctcggcgaa ggtgtaggtg
      181 tcgggaaaca gatagccttc ggccagcggc gcgttgatgc cgatcgaggc gaggaaggcg
      241 ggatcggcca caagcgcaag gaaggcgtcg gcccggacga cctcgttttg ctgcaggcgt
      301 tcggcgatct ggtagatatt gtatccctcg gggatggtca ccttgcggcg gatgatgtcg
      361 ccgcggttga tcttctggac gatctcgaag gcggtggtgc ccgggtggac cttgtagcgc
      421 ccggcgatga cggcggtgcg ccccgtcgcc tgcgcaatcc ggtacatgaa ggcctcgctg
      481 cggacgatat tacgctcggc gagcgcatgg gccacggagc gcatcgtgtc gcctttcttg
      541 acggcgacga tctcggttac caggtccgag ggggcggaat tcatgaacat gaacccgccc
      601 gcaatcacga ggacgatccc gacggcgatg aggccggatg tgaacttttt cattggtgcg
      661 cgccttccat gggtgctgta ccggcaggta ccggacgggg ggcggttcgt caaggaaaaa
      721 tgcggtgtaa ccggatcgac gttcgggaac ccgaaatgat tcaatacgca tgtttttatt
      781 gaaaaaatcc ccagttcgtg tatactttga aataccgcca tgggtgccga tatatcaaat
      841 gaagcagtgg aatcggttcg catggctggc gcacccgtga atagactgtc catcgtcata
      901 gcgggggcgg attgagcctc ggcccagtaa aaggccgtta tcggtggcaa agcccatcat
      961 tcgcgatgtt gattcggcat tcgccgtgtg cccgcacaga tctgggagac cggcagggag
     1021 gcgtgatgaa ggtattatgc gcagttgcaa ttgtcggtat tgccatgttt acggcatgta
     1081 cgtcgagcga tgatgatgta ttcaccggaa ccgggaccga tgttcgcgtg gtgaccgtcg
     1141 cgggcgacgg cacggccgac ctgacagacg gcgtcggcac cgctgcgcaa ttcaaccaac
     1201 cggacggggt cacggtgaac aagacgaacg gactcatcat cgttggggac accggcaata
     1261 atgcgatccg gacgatcgcc gcagacggca ccgttgttac gcttgcgggg cagaaaacac
     1321 ccggatttgc tgatggcgac ggggctgccg cgcagttcga ccagcccgat ggcgtcgccg
     1381 tcgattcgtc cggcaatgtg tacgtagccg ataccggcaa caaccgcatc cgcaagatca
     1441 ccccgggcgg gaccgtgacc accatcgccg gcgacggcac cgccgacttc aaggacgata
     1501 acgggacgag cgcgcagtta aacggtccta ccggaatcac cgtggactcg agcgggatta
     1561 tcttcgtggc cgattccggc aacaacgtga tccgcaggat cgattccgcc gggaacgtca
     1621 ccaccgtggc cggggatatc gtagggcagg ctgccggaga cgcggacagc gccaccggca
     1681 cggacgcgcg gttcaagaag ccccacgcgg tgaccgtcga ttcatcgggc aacctgtacg
     1741 tgacggatca cgagaacaat aaaatcaaaa agatcacccc ggggtttgcg gtgagcacct
     1801 acgcgggcga cggcaccgcc gccttcctcg acggcgtcgc gtcggcgagc cgcttcaaca
     1861 ggccgcacgg cctcaccgtc gacggcgccg gcaacatcgt caccggggac gagctgaaca
     1921 accgtatccg cttcatcacc cccagcggcg tcgtgcagac ctatgccgga aacgggaccg
     1981 cgggctacgc ggacggatcg gcggcatcgg cgcaattcaa acagccccgc ggcatcgcgc
     2041 tcgacacgtc cggcaatatc ctcatctgcg attacgacaa taaccgcatc cgaaagatcg
     2101 tccggtagcg gcgcgtgggc ccgctcacgc tggtctatct ccgtgacggg agaatgatcc
     2161 tcacggcgga gccctttgcc gattggcgag aagcgcagga ccgctatgcg gactacatgt
     2221 cctccctcgc atttgagaca atcgaagcgg tgcggcgcta tcttgttctc gaataccgca
     2281 tcaccgagga gcgggccgcg gtaattaccg ccggatggca gtccggggaa gtggagatcg
     2341 aggtgtgatc gctatccgtt cttcatttgg ccggtagtcg ttcggatcaa cctcttatca
     2401 acctctattc aagctctatt ggggcctcaa ttatggagcg atccgtcaat catgaagcgg
     2461 ttgtcgtatc gatgcggtcg ctatcggcgc cctgctggtg ttacgccgat cccgcgcggt
     2521 agggccggat ctgcgcgagc gcctcgtcgc gatacttgaa gaagatgaga tcggacgggt
     2581 ccgtgacggc gaatgttttc atattgttct tgacgaatag gaattatgtc gtataggtgc
     2641 agcacggtgc cgcgtggtat ggcggacatc gcagcacgtg gaatcggcac gatacaggat
     2701 gaaacggaat cggcaacaac attttattcg ggcggcctgg ggcctgctct tcgtgtgcgc
     2761 gtgtattgcg ctgcccgggg attccggcac ggtgccggag aagaatgccc cgtactacaa
     2821 caagaccggc tgggagcacc tgaaaaccgg caatgcgcac aaggcgatcc ttgccttcag
     2881 aaacgcgctg cgccagaacc cgcgctacaa ggaggcgatc gtcggcctgg cgcagggata
     2941 cctttccacc gaggcctacg acgaatccct gaagctcttc gaggacgccg tcaagtacga
     3001 ccggaagaac agcgccatcc tgtccggcaa ggggatggcg cttgcgggcc tgggacggta
     3061 ccacgaggcg ctggccgcct ttgacgaggc ggtgaaactc tccgaggaaa acctcgatgc
     3121 gaagtacggc ctcgcgcgcc tgtattatct catggacaag cgcttgtggg ccaaacgcaa
     3181 gctcgagacc atctttcgca gcaatccctt tcattatgat tcgttgctgc ttgccgccga
     3241 gatcaaagcg gtagaaaagc gactggacga ggcgacggcc ttcgtgaaaa aggccgtgga
     3301 gtccaatccc gaactgccgc agggctatgt gcgcttcggc gacatctatc tgcgcgaata
     3361 cctgaagcgc tacgatgacg acttcgtcgt caaggcggtg gaggagttcg gccgggcgtt
     3421 ggccctgcag cccgaccatt tcgccggcaa ccgctacctg gggattgtcg cgctaatcaa
     3481 ggggaacatg ggggaggccg ccgtgcattt tcggaagtgc ctcgccgtga acccgaagag
     3541 cgtgcccgcg ctctacggac tcgccgtcgc gctcgcgggg agcggctccc tcgatgaggc
     3601 ggagaagcag tacgcggcgg cgctcgcggt cgcgccgtcc aacgggatcg tgcaaaacgg
     3661 cttcgaggac tttctggtcg gcagcggttt cgcaatgggc aacccgtccc gcgtcgatct
     3721 ctcgcgggcc cacctgacca ccgcgcgcga ctgcatgaag aaaaaactcc cggtgcagtc
     3781 gatctatcat ctgcgtcgga cgctcctgct caaccccctg gaccgcgagg cgcgcgaggc
     3841 gctgcgggac tactactatg cgcaggacta ttaccgcttt tatgtcgatg agatcggcac
     3901 gctcgtgaag ctgtatccct ccgagaaata ccaggaggcg ctggagatcg ccgtcatgaa
     3961 gcggcgcgat cgtctgtacc agcgcgtggg gtacgggacc gagccgccgc cgcgcgacgt
     4021 cccgtcggtg ttcgtgctcg atctgcaccc gcgcggggag atgcccgcgc actacgacgc
     4081 cggccgcgtg atcgccaacg ccatatcgtt ctcgctggcc cagtggggac ggatgcgtcc
     4141 cgtgggaatg aaggaccggg ccgaaaccct gcgcggcatc cgcttcgacg acgagtatat
     4201 cgaaaagtcc ctcgagcgca tcaacgaccg cgccggaaag ggagcgctcg ggccggtcga
     4261 ctatgtgctc tacggcgact tccgcgaggg ggtcggtttc gtccacatca ccctcaatct
     4321 cctgaaccgg accaacgggg tcgtcatcac cagcttcact ctttccgagc tggggcgcaa
     4381 caaccttccc gaggtcgcgt tgcgcgcggc gcgcaaggtg tacgagaaga tccccttccg
     4441 cggccgggtg ctcaagatgg acgaggacaa gccgatcgtg aacctggggg gcttcgacgg
     4501 gctcaagaag gacgacatct tcgtgattta caaggacgcg ccgcgcgatt cccacgcggg
     4561 cggcatcggg aaaaagatga agatcctgct caccgtgggc gaggccgaca cgctggtttc
     4621 ggcgctctcg gtcgagcggg cgcaggacct cgcgatggtc gatgtcggcg atgcggtgta
     4681 tccgctggag aagcgacggg cgaagcgctt gaaatgacgt aagcgcagag gacgggttgc
     4741 ttcgcgaagc tctcgatgtc attgagtgaa acgacacgtc atattacggt acgagtggca
     4801 gggaggaata catgaaacgg atggcggtat acggcattgc agtggtgctg atggcggcgt
     4861 gcgccgctgg ctgcgggaag gggtcctatg gcgggctcaa atacggcgac atcgtcccag
     4921 gcgactggct gcaggccaat gtcgttgacg cgaagaccat gaagcgcgcg atgtggttcc
     4981 agatcttcga ccagaagggc gacgattcca tcgtgagcta ttacaagaaa tcgtccgaaa
     5041 cggttgcagg ctatcccgcg aacgtgaaca aggataaatg gatctggatc ctcatcaaca
     5101 atcgcatcga gatacggctg ttggccgacg acgagcaccc ggacttccag aagaccgaga
     5161 agcttgccga attcctgaag cgcttcgacc tcaccggcct gtcgaagatc gacggcgcca
     5221 aggtgcaggg ctcggcgttg cgcaagtaca tgcccaagct cggctcgtaa tcgtcgacgc
     5281 gcccgaggcc cggcccgtgc gcatggtgca aggagtgaag caatgaaaaa gatcgcgatc
     5341 accgcattgt tcgtgattgc cgtcccgctg gcgggatccg cccatcgcct cgagtggaac
     5401 atcccgcagg gcaagcgtct tgagatcgtt cgcacggccg aggtcgaata ccggaagaac
     5461 tcccgcgtcg agaagaccta taccgagcgg aacatcatcg acttcacctg ccaggagcgg
     5521 accggcgacg ggagccgcgt gaaggggcag tttaccgtgt acagccgggg cgcgggcgag
     5581 gaggtgttca acctccgcga gcgcttcgat tccgagttca ctatcgcgcc cacgggccgc
     5641 ttcacggtgc cgccgcagtt catcatgccc aatattcgcc acgttcccac ctttcccgcg
     5701 gggggcgtct ccgttggggc gaagtggtcg gcccccgccg aggtgctcat caacgccttc
     5761 aagccgccgc tcatgctgca gctcgatgcg cagtatttcc tcacggcggt ggagaagcgc
     5821 ggcgggacga cggtgggcgt catcaactac tatctccata tcgacaagga cctcgtgggc
     5881 aaggtcacgg gcgagaacta tcccgcgcgt atcttcgggt attacttcgc gacgctcaac
     5941 tgggacctgg agcgcaatct gcccatcgac tcgatcgaga aatacaccat catgttcctc
     6001 ttcgggagcg accgcaccgg gtacgacagc ctcgagtttc gcatgaacat cacctcgcag
     6061 aacaagctct acgagaacgt gggcaaggac gagaccgatc gccaggcgaa ggacctcgcg
     6121 gggaagcttc cgtccggcag cggcgttacg gtggacacgc atgagaaggg gctggtgctt
     6181 cgcctcggcg aggtcctctt cgacgtcaac tcggcgcgga tacgggaaga cgcgaagggg
     6241 aatctggacg ttattggaaa gctgctgaag gaacggtatc ccgaccgcga gatcatcgtc
     6301 gaggggcaca ccgacaatac cggcgagtcg ccctacaacg acaaactttc gcatgaccgc
     6361 gcgaaatcgg tgggcgccca cctcaagggg cggatcggcc acaacaagct ttcgttcatc
     6421 ggccacgggc cgcgcaagcc catcgcggac aaccggacca aagagggccg gcagaagaac
     6481 cggcgggtgg agattatcat caagttgaat tgaaggactt tgcgagttcg ttcccgccat
     6541 tccaaaggcc catttccagt agatgccgcc tgttgtcgta cgttgtgtga tctcgatggc
     6601 cgctccttcc cgtgattcca aataaaatcg attgcttttt cgcgtagcgt atggtatcat
     6661 acatgcgccc ggcatatttg tgcggcgcac tctcgatatg aatccgttac ccggctatac
     6721 tatcttcgaa atgatcgacg agacctcccg gtcgctggtg catcgcggtc ggagggactc
     6781 ggacggtacc ggcgtcgtca tcaagctcct gaaaaccgac cgggcgacgc cgacggacct
     6841 ggcgcgtttc cgacaggagt acgcgatcat ccgggagatc gacgacgagg gcgtcgtgcg
     6901 gacgctcgac atcatcgact gcgatggcgg catcgcgatc gtcatggagg acttcggcgg
     6961 ggtttcgctg aagcgggctc tgcgggaagg gaaggccgac ctggacttcg tccttgggac
     7021 gggagcgtcg attgcctcgg cgctggggcg gctccacggg cgcaagatca ttcacgggga
     7081 catcaagccg ggaaatatca ttatttcggc tgattccctc accccggctc cgccgcgccc
     7141 tctcccgggg ggagagggct ctgtaaaaat tgccgacttc ggtatcgccc gtgcggtcac
     7201 ccgcgccgac gagaacatca atgatccgaa cgtgatcgag ggtacgctcg cctacatgtc
     7261 cccggagcag accggccgca tgaaccggac gatcgatcat cgtacggacc tctattccct
     7321 cggtgtcacc ctatacgaga tgctcacggg cgaggtgccg ttccgctcgg cggacccgat
     7381 ggaggtgatc cacgcgcaca tcgcgaagaa gccggtgccg ccgcacgagg cggggaggcc
     7441 ggttccgtcg gccatctcgg caatcgtgat gaagctgctg gcgaaggcgg cggaggagcg
     7501 ctaccagtcg gcggcggcgg tcgcggagga cctcgggaga tgcctggaga tgctccgctc
     7561 gaacggacgc atcgacgatt tcgccattgc cgcaaatgac atagcggctg attttcgctt
     7621 ttcgtacgtg ttcgtagggc gtgagaagga gatcggcgag ctatacgcgg ccttcgagcg
     7681 ggtggccgtc aacatccgcc ccaatctccc cgttggagcg ggcgaacggt cgcggatgga
     7741 gctggtcctc gtggcgggcc cgccggggat cggcaagacg gcgctggtga acgagctgca
     7801 gcgcccggtg ctggcggagc gagggtattt catctccggg aagttcgagc agtacagccg
     7861 cgacatcccg tacggcgcgc tggtgcaggc cttccggtcg ctcgcggcgc ggatgctcgc
     7921 ggagagcccg gagcgcctcg ctgcctggcg ggagcggctg aaggccggct tcgggaatat
     7981 cgggaaggtg atcacggaca tcgtgcccga gctcgcgctc gtcgcgggga atcagcccga
     8041 cgttcccgag cttgggcccc aggaatcgca gaaccgcttc aactacgcct tccggaactt
     8101 cgttgcggcc atcacggccg cagggcccat cgtcctcttt cttgacgatc tgcaatgggc
     8161 cgacatggcg agcctgaacg cgctcaggac cgtggcggct gacccggaca tcgggaatat
     8221 cctgataatc tgcgcatacc gggacacgga ggtgggcgcg gcgcacccgg cgatgctttc
     8281 gttggacgac atgcggaagt cgatgaccat cacggagctc tcacttccgc cgcttgagac
     8341 ggaccgcgtg aacgacatta tctcgctcct cctgaaaacc tgcagcggag agacggcgtc
     8401 gcttgcggag ctggtgttcg ccaaaacagg tgggaatccc ttcttcgtgt accagttcct
     8461 ggggctcctg cgcgaccgcg acctgctcaa gacgggaagc aggggctgga cctgggacat
     8521 ggacgcgatc cgctccctgc cggtcacgga gaacgtggtg gacctcatgg cgggaaagat
     8581 atccatgctc ccggaggaga cgcggaaggc gttaagcgcc gctgcgtgca tcggtacggt
     8641 ctttggtctg gacctgctgg cgaaggtgat cgatgtatcg attgacgatg cgctccggtt
     8701 gatagcgccc gcgatcatcg agggctttct tgttgcggag gaagacgagt atcgtttcac
     8761 tcatgataaa atacaggagg tatcgtacat ccttcttccg gagaacgatc gaaacgcgct
     8821 ccattatcga attggcaaca tcttgctcaa tacaacatcg gaagatatac tcgacgagaa
     8881 aattattccg atcgccgatc aactaaattg cggatcgtcc atggctcatc gggatgacga
     8941 gaaagttcgc ttggccggtg tgaacctaaa ggccgcccga aaggcgtgga gggccgccgc
     9001 gttccaatcg tcgtaccgct acagtggaag cgggatcgta aatctccccc ccgattcatg
     9061 ggtagtgcga tatgatcttt gtcttgagct gttcaatatc ggcgtgcagg ctgcgtacct
     9121 ttcggacgat tacgatggga tgagccgcct tgctgaggta gtattgcgca atgcgcgacg
     9181 cgtgacggat tgcacaacag tgtacgca
//
LOCUS       DAORHF010000146         9177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_6977_length_9177_cov_5.634839, whole genome shotgun sequence.
ACCESSION   DAORHF010000146 DAORHF010000000
VERSION     DAORHF010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6977_length_9177_cov_5.634839"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PKJ16_12105"
     CDS             <1..523
                     /locus_tag="PKJ16_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HNV47776.1"
                     /translation="GRLTERKMFLYFQARTMGLSGDAYRRMAQRFFHEKGRHHVYAGA
                     LEMIDAHRDRGIHTVIITGQDNFITEPFCDRLGVDGFISNRRIERGRRLGGFHTPNCY
                     GAGKITLAGDYARTRGCALRECSFFTDSISDLPLMREVGHPVAVNPDPELRSIAENAK
                     WRICSFDTVVSLN"
     gene            complement(601..1629)
                     /gene="fliM"
                     /locus_tag="PKJ16_12110"
     CDS             complement(601..1629)
                     /gene="fliM"
                     /locus_tag="PKJ16_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HNV47777.1"
                     /translation="MTEILSQDEIDALLTAISTGEVDTTDYTATKEQRKVKIYDFRRP
                     DKFSKDQIRTLQMMHETFARLTTTALSAQLRALVSVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAVLEIDPSITFTIIDKLFGGTGESSAKISRELTDIELSVMEGIIVR
                     ILGNLREAWSNVIDLRPRLGNIETNPQFAQIVPPNDMVVLITLETKVGEVEGMTNLCI
                     PYITIEPVISKLSAQYWYSSIRKGTTDENMSIIQERLEAVELPIVAEIGEVEIAMQDV
                     LSLQVGDVVKLPQTKVNADMVVKIGGRRKFKCRPGLIGTRIAIQIGEEIEEVPDELLI
                     AKRSEEDL"
     gene            complement(1632..2582)
                     /locus_tag="PKJ16_12115"
     CDS             complement(1632..2582)
                     /locus_tag="PKJ16_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47778.1"
                     /translation="MKHIISRIIPTGLFMLMALPALWAPAGAQESSDGEPAFRVHIRM
                     DRFTFFSDEPVVLHLHVTNNAAGRVSFRAFDELYSTYRPVVYDMNGREAETTVPYRLM
                     NKRLGDILSAHTPRTVELDAGESFVYTIDLRRLYSLPENRSFRVKGFFFPDAANPAAV
                     PGENTIQFRVVREHSAAKKSGVDRLPRGIAPAEIVTLMLQAEKAGRWQTMMKYIKVDS
                     FIAAFPEFSNNYSRADEREKLILEEDFIRFLSKRRSDYILDFTILNESIREQTSAFVD
                     VRIERFGPRLPLIYRYRYRLERHRDYWLVTNVEVSVIKVD"
     gene            complement(2794..3120)
                     /locus_tag="PKJ16_12120"
     CDS             complement(2794..3120)
                     /locus_tag="PKJ16_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47779.1"
                     /translation="MDLHIRRAGEHAILDIIGNAHLYTINSLNKALLGLIEERESSIV
                     VDARNIEFFDSSFLASLLMAKKKVEAYNGRIALMNINEPLQRLLEIANMDKRFRIYES
                     EEHLVQ"
     gene            complement(3224..4798)
                     /locus_tag="PKJ16_12125"
     CDS             complement(3224..4798)
                     /locus_tag="PKJ16_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HNV47780.1"
                     /translation="MKLVIDDIKLQPHEPEEALGRILSASFGISDGYPFVIIKRSLDA
                     RKKGRIVYRYRLMLDLPEPEARWLLEREGVSIYREPDMPAVVKNLNGLRVIVIGAGPA
                     GLFCALRLIEHGAIVEILERGRPVEERLGDIRLLERDGTLDPESNVLFGEGGAGAYSD
                     GKLTARTRRPESAWFFETLVSLGAPPEIRSDAHPHLGTDRLAAILTGIRARITAAGSR
                     IRFSTRIDDILAEHGTVAGVRTAAGEEIRCGRVVLATGHSARDTYAMLAARGLSLRRK
                     DFAVGARIEHPAELINAIQYGPSARNGSLPPAEYSLAWKNPATGRGVYSFCMCPGGMV
                     INASSEPGMLCTNGMSRSKRDGDFSNAALAVTIRADDFPGGPLAGIDFQRDIERRAYA
                     AGGGDFRAPAIRVQSFLAERGDATLPALSYCPGARPGDPRDYLPPFVIAELLQALPIF
                     DRRMRGFAGSDAVLIGAETRTSSPVRILRDDSFQSVSLAGLYPAGEGAGYAGGIVSSA
                     VDGIRIADAIARQAQA"
     gene            4914..5534
                     /locus_tag="PKJ16_12130"
     CDS             4914..5534
                     /locus_tag="PKJ16_12130"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47781.1"
                     /translation="MEKIKRKSAKVLDKQERKKNHIIWAAFNAIAKIGYINLTIDEVA
                     KEAGVSKGVVHYYFDTKENLMLEVMFTFVQFFVVSLNFAVSPALAPTERIKGIMRFSM
                     KIALNHKEYIRVIIDYWAQVNINVNLRDIYVFYQNQIIAYIAEIIDQGKAAGEFKEVD
                     SESAAEIILSEIGGFAIRYTWDEAATEETFADKIESLVDTILRIVR"
     gene            5714..7420
                     /locus_tag="PKJ16_12135"
     CDS             5714..7420
                     /locus_tag="PKJ16_12135"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47782.1"
                     /translation="MNSQAKLQELLTIIMDIAKDMVEAEGSSLLLTDLETEDLIFNIV
                     VGEKGNIIRGEKVPKGMGIAGAVAESGQCLIVDDAQNDPRHFKEIDQLSVFTTRNILA
                     VPMKVMDKLIGVLEVVNSIGRPTFDEWDKKLITYIADQAAIAIHNRMLYDDLMARVEE
                     LTSLYEISQNLSFADTEETIFQNVVQTVAKSLGVEKASVMIYDEARDRLLVRAHLGLP
                     GAVTDRAEVEVMESICGYVFRSGDPLIVSDIDKELSFPFLKRKDHYRTKSFISLPIRH
                     KDRTIGVISLADKKSRACFDVYDLRVLTTIGTHVAEVYENIRYQRSVENQRRLAQEID
                     IASELQRKILPAMPATVGDHRLAAFNRPAKDVGGDFFDFKMLGESKYSVLVADVSGKG
                     IPAALFMGSSRNLLRAEARINNQPGMLLHSSNRYIYEDSEHGMFVTLFYMVVDMHNSL
                     ITYGSAGHNDQLIIKRTTRDVVRLNARGMALGMQPDSEYEEKVTFFEPGDWAVLFTDG
                     VLEYLGEIDIDAGEARLIDIAMQYLDRGPQEFIDCLRGIIEETKEQSDFIDDFTIVAI
                     QL"
     gene            7420..8295
                     /locus_tag="PKJ16_12140"
     CDS             7420..8295
                     /locus_tag="PKJ16_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47783.1"
                     /translation="MRRAACIIILLAVAVPLGSQMPDWFTVRDRDGNEYFMDRNGKIW
                     TSGSPDFSYKPASIEGLDYFVTQGVDLIRRHYPAEGLTMLKSVMALPVSNKTIYDAQV
                     KASRAIQALQRREGTRYTALSERASLVYYREGEAVTLHNDRMNWTIRFPRPVTVIRRS
                     ARTRNGYLRQGVLVGVTFAPRPPEAASEAPKYDALVAIDSEQFPFELTSVKKLEAHWS
                     NALGGAETDRHLDHRAPDRAVYHCRDKRPGEYSWYEGYYVNGRFGTMVRMIMETGAEG
                     RHGRAMLQALKDFKH"
     gene            complement(8308..8727)
                     /locus_tag="PKJ16_12145"
     CDS             complement(8308..8727)
                     /locus_tag="PKJ16_12145"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV47784.1"
                     /translation="MRRTRSIDKRDYGRLKTVNLKVDYRIKGSETPTTVEVINLSTGG
                     LCFLRNTIINKGEFIQVRFPFKTRKVIMNAQVVRIDGREVGVKFTDSEKEIERFINAF
                     NEEYPSIRTQESADGDRVFGETYQRLKGMRTEKEPKP"
     gene            complement(8724..>9177)
                     /locus_tag="PKJ16_12150"
     CDS             complement(8724..>9177)
                     /locus_tag="PKJ16_12150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV47785.1"
                     /translation="QDVDAILAIINHYAVRGVILPRDRADVLKSLPHFLVADRNGTVV
                     GIVSRHDYGAHLKEVRSLAVADTERRGGIGTLLVTGLVERLRRDFPDAKIFVLTAVPA
                     FFTRLGFCAVEKSTLPEKIWKDCRYCAHQDNCDETALTLSNARGTMQP"
BASE COUNT         1743 a         2782 c         2930 g         1722 t
ORIGIN      
        1 cggcaggctc accgagagaa agatgttttt atactttcag gcgcgcacca tgggactcag
       61 cggggacgcc taccgccgga tggcgcaacg cttctttcac gaaaaggggc ggcaccatgt
      121 gtatgcgggc gcgttggaga tgatcgacgc ccatcgcgat cgcggcatcc acacggtcat
      181 catcaccggg caggacaatt tcatcaccga acccttttgt gaccgtctcg gcgtggatgg
      241 gtttatctcc aaccggagga tcgagcgcgg ccggcggttg ggcggctttc atacgccgaa
      301 ttgctatggg gcgggaaaga tcaccctggc cggcgactat gcgcgcacac gcggatgcgc
      361 attgcgcgag tgctcctttt tcaccgacag catctccgat ttgcccttga tgcgcgaggt
      421 cgggcacccc gtcgccgtca atcccgatcc ggagctgcgc agcatcgccg agaacgcgaa
      481 gtggcgcata tgctctttcg atacggtggt gagcctgaac tgatggcggt tgattcgcgg
      541 ccgcttcgaa taaatccgca gaagtggaaa agcggggccg ctcgatggca ttgacgtcgc
      601 ctagaggtct tcctcgctgc gcttggcgat gaggagctcg tccgggacct cttcgatttc
      661 ctcgccgatt tggatggcga tgcgcgtgcc gatgaggccg ggccggcact tgaacttccg
      721 acgaccgccg atcttcacga ccatgtcggc gttcaccttg gtctggggga gcttcaccac
      781 gtcgcccacc tggagcgaga gcacgtcctg catggcgatc tccacctcgc cgatctccgc
      841 gacgatgggc agctccaccg cctcaagacg ctcctggatg atggacatgt tctcgtccgt
      901 ggtccccttg cggatactgg agtaccagta ctgcgccgag agcttggaga tcaccggctc
      961 gatggtgatg tacgggatac agaggttggt catcccctcg acctcgccca ccttggtttc
     1021 aagtgtgatg agcaccacca tgtcgttggg cggcacgatc tgcgcgaact gcgggttggt
     1081 ctcgatattg cccagccgcg gccgcaggtc tatcacgttg gaccacgcct cgcgaaggtt
     1141 cccgaggatg cgcacgatga tgccctccat cacggagagc tcgatgtccg tcagctcgcg
     1201 gctgatcttc gcggaggact cgcccgtccc cccgaagagc ttgtcgatga tggtgaaggt
     1261 gatggacgga tcgatctcga gcacggccga tcccttgagc gggtccatgt tgatgaccgc
     1321 cagcgtcgtc gggttcggga tggagcggat gaactcctcg taggtgagct ggtccaccga
     1381 ggccacgtgc accgatacga gcgcgcgcag ctgcgccgag agcgcggtgg tcgtgaggcg
     1441 cgcgaaggtc tcgtgcatca tctgcagggt gcggatctgg tccttggaga acttgtcggg
     1501 acggcggaag tcgtagatct tgaccttccg ctgctccttg gtggccgtgt agtccgtggt
     1561 atcgacttcc cccgtcgata tcgcggtcaa tagggcatcg atctcatcct gcgagagaat
     1621 ctcggtcatg gtcaatccac ctttatcacg gatacctcaa cgttggtgac gagccagtag
     1681 tcccggtggc gttccagccg gtagcgatac cggtagatga gcggcaaccg cggaccgaat
     1741 cgctcgatcc gcacgtccac gaacgcgctg gtctgttccc ggatgctctc attgaggatc
     1801 gtaaagtcaa gaatataatc gcttcgccgc ttcgagagga atctaatgaa atcctcctcg
     1861 agaatcaact tttcccgctc gtcggcccgg ctgtaattgt tcgaaaattc ggggaaggcg
     1921 gcgatgaagc tgtcgacctt gatgtacttc atcatggtct gccagcggcc ggccttctcc
     1981 gcctgcagca tgagggtgac gatctccgcg ggagcgatcc cgcgcgggag gcggtccacg
     2041 ccgctctttt tggccgcgct gtgctcgcgc accacgcgga actggatggt gttctccccg
     2101 ggaacggccg ccgggttcgc ggcgtccggg aaaaagaaac ccttgacccg gaaggagcgg
     2161 ttttccggca gcgaatagag gcgcctgagg tcgatggtgt acacgaagga ttcgcccgcg
     2221 tcgagctcga cggtgcgcgg cgtgtgcgcg ctcaggatgt cgcccaggcg cttgttcatg
     2281 agccgatagg gaactgtggt ctcggcctcg cgtccgttca tatcgtacac gaccggacgg
     2341 taggtgctgt acagctcgtc gaaggcccgg aaggacaccc gcccggccgc attgttggtg
     2401 acgtgcaggt gcagtaccac cggctcgtcg ctaaagaagg tgaagcggtc catgcggatg
     2461 tgcacccgga acgcgggctc cccgtccgag gattcctgcg cacccgcagg cgcccagagc
     2521 gccggcagcg ccatcaacat gaaaagcccg gtgggtatga tgcggctgat gatatgtttc
     2581 atgcgttgtg ccatatacgc gcgttttccc gtaccctgcc gacggaactg gcaggtgtat
     2641 actttattat cggaaaaacg ggattatgtc gtaataaata aaattaccgt cagtatggat
     2701 gtatgtcggg gggtgtgagt gcgatcggtc gctttcgggg tattgcgggc cgcctctcaa
     2761 tcgcactatc cgaacggcgg aggggtgatc ggcttattgc accaggtgct cttccgattc
     2821 gtatattctg aaccgcttgt ccatattggc gatttccagg agccgctgca gcggttcatt
     2881 gatgttcatg agcgcgatgc gtccattgta ggcctccacc ttctttttgg ccatcaggag
     2941 cgaggcgagg aaggaggaat cgaaaaattc gatattgcgc gcgtccacga cgatgctcga
     3001 ttcgcgctct tcgatgaggc cgagcagggc cttgttgagg ctgttgatgg tgtagagatg
     3061 ggcgttgccg atgatgtcga ggatggcatg ttcccccgcc cgtcgaatgt gaagatccat
     3121 gtgtcctccg catgaatgct gtctgtagcg ttatgataca aatagatatc acatgttcac
     3181 agcccgcatc aagtcaattt cagcgatgtc ggatattgat cggtcaggcc tgggcctggc
     3241 gcgcgatggc gtcggcgatc cggatgccgt ccaccgccga gcttacgatg ccgccagcat
     3301 agccggcccc ctctccggcg ggatacaggc ccgcgagcga taccgactgg aaggaatcgt
     3361 cgcgcaggat gcgcacgggc gacgaggtgc gcgtttcggc gccgatgagc acggcgtctg
     3421 atccggcgaa gccgcgcatg cggcggtcga agatcgggag cgcctgcaat agctccgcga
     3481 tcacgaacgg aggaagatag tcccgcgggt cgccgggacg cgcgccggga caataggaga
     3541 gcgcgggaag cgtcgcgtcc ccgcgttccg cgaggaagga ttgcacccgt atggcgggcg
     3601 cgcggaagtc cccgccgccc gcggcatagg cgcgacgctc gatatcgcgc tggaagtcga
     3661 tcccggcgag gggcccgccg ggaaaatcat ccgcgcggat cgtgaccgca agtgccgcgt
     3721 tcgagaagtc gccgtcgcgt ttcgagcggc tcatgccgtt cgtgcacagc atcccgggct
     3781 cggaggatgc gttgatcacc atgccgccgg gacacatgca gaaggagtag acgccccgcc
     3841 cggtcgcggg gtttttccag gcgagggagt attccgcggg cgggagcgat ccgttccgtg
     3901 ccgatggacc gtactggatt gcgttaatga gctccgcggg gtgctcgatg cgcgcgccca
     3961 cggcgaaatc cttccgccgg agggaaagcc cgcgtgcggc aagcatcgca taggtgtcac
     4021 gcgccgagtg gccggtcgcc agcacgacgc gcccgcaacg gatctcctcg cccgccgccg
     4081 tgcgcacgcc cgcgaccgtc ccgtgttcag cgagaatgtc gtcgatgcgc gtcgagaacc
     4141 gtatgcgcga cccggctgcg gtgatgcgcg cgcggatgcc ggtaaggatc gccgccagcc
     4201 ggtcggttcc caggtgcggg tgcgcgtcgg agcggatctc cggcggcgct cccagcgaga
     4261 cgagcgtttc gaagaaccag gcgctctccg gccggcgggt gcgcgcggtg agcttgccgt
     4321 cggaataggc gcccgcgccg ccctcgccga agagcacatt gctctcggga tcgagcgttc
     4381 cgtcgcgctc caggagccgg atatcgccca gccgctcctc gacgggacgc ccgcgctcga
     4441 ggatctcgac gatagcgccg tgctcgatga gccggagggc gcagaagagg cccgccggtc
     4501 ccgcaccgat aactatcacg cggagcccgt tcaggttctt cacgacggcg ggcatgtccg
     4561 gctcgcgata tatcgacacg ccctcgcgtt cgaggagcca gcgggcctcg ggctcgggga
     4621 ggtccagcat caggcgatag cggtacacga tgcggccctt tttccgggca tcgagcgacc
     4681 gcttgatgat gacgaatgga tacccatccg aaatgccgaa cgaggcggac aggatgcgcc
     4741 ccagggcctc ctccggttca tgtggctgca gttttatgtc gtcaatgacc aatttcatgg
     4801 gcaaaaatca tacttcgtaa cacaaatatc aaccgctgta cataaaaaaa agtggacaaa
     4861 gggattaatt ttttacacga taccgcaacc agcgattgta acgcccatat atcatggaaa
     4921 agatcaagcg caaaagcgcc aaggtgctcg acaagcagga gcggaagaag aaccatatta
     4981 tatgggcggc gttcaacgcc atcgcgaaaa tcgggtacat caacctcacc atcgacgagg
     5041 tggcgaagga ggccggggtt tccaagggcg tcgtccacta ctacttcgat accaaggaga
     5101 acttgatgct cgaggtgatg ttcaccttcg tgcagttttt cgtggtgtcg ctcaatttcg
     5161 cggtgtcccc ggcgctcgcg cccacggagc gcatcaaggg gatcatgcgc ttctccatga
     5221 agatcgcgct caaccacaag gaatacatcc gcgtgatcat cgactactgg gcccaggtga
     5281 acatcaacgt gaacctccgc gacatctatg tcttctacca gaaccagatc atcgcctata
     5341 tcgccgagat catcgatcag ggcaaggcgg ccggggagtt caaagaggtc gattccgagt
     5401 cggccgcgga gatcatcctc agcgagatcg gcgggttcgc aatccgctac acctgggacg
     5461 aggccgccac ggaggagacc ttcgccgaca agatcgaatc gctggtggac accatccttc
     5521 gcatcgtccg ctaggggcga ccggcgcgcg aattattaat tgacatcggc gaacggtatg
     5581 ccgacactac aagcggcggc gtgctgatgc gcgcggggcg cgtcgcacaa ccgtacagtc
     5641 catagccgga cgggggatcg tcatcggaag aaaagtcatc aacctgtcgc agatcgggtt
     5701 catcaaccgc aagatcaatt cccaggcgaa gctccaggag ctcctgacca tcatcatgga
     5761 catcgccaag gacatggtcg aggcggaggg ctcgtcgctc ctgctcacgg acctcgagac
     5821 cgaggacctc atcttcaaca tcgtggtggg cgagaagggc aatatcatcc gcggcgagaa
     5881 ggtgcccaag ggcatgggca tcgccggcgc cgtggccgag agcgggcaat gtctcatcgt
     5941 ggacgacgcc cagaacgacc cgcgccactt caaggagatc gaccagctct ccgtgttcac
     6001 gacccgcaac atcctggccg tgcccatgaa ggtgatggac aagctcatcg gcgtgctcga
     6061 ggtggtgaac tcgatcgggc gtcccacctt cgacgagtgg gacaagaagc tcatcaccta
     6121 catcgccgac caggcggcca tcgccatcca caaccgtatg ctctacgacg acctgatggc
     6181 gcgcgtcgag gagctcacct cgctctacga gatctcgcag aacctctcgt tcgccgatac
     6241 ggaggagacg atcttccaga acgtggtgca gaccgtcgcc aagtcgctgg gcgtggagaa
     6301 ggcgtcggtg atgatctacg acgaggcccg ggaccggctt ctggtgcgcg cgcacctggg
     6361 gctgcccggc gccgtgacgg atcgcgccga ggtcgaggtg atggagtcca tctgcggcta
     6421 cgtgttccgc agcggcgatc cgctgatcgt cagcgacatc gacaaggagc tctcgtttcc
     6481 attcctgaag cgcaaggacc attatcgcac gaaatcgttc atctcgcttc ccatccggca
     6541 caaggaccgc accatcggcg tcatcagcct ggccgacaag aagagccgcg cctgtttcga
     6601 cgtctacgac ctgcgggtgc tcaccaccat cggaacgcac gtggccgagg tgtacgagaa
     6661 catacgctac cagcgcagcg tggagaacca gcgacggctc gcgcaggaga tcgacatcgc
     6721 ctccgagctc cagcggaaga tccttcccgc catgcccgcg acggtggggg accatcggct
     6781 cgcggccttc aaccggccgg ccaaggacgt ggggggcgat ttcttcgact tcaagatgct
     6841 gggcgagagc aagtactccg tgctggtggc cgacgtttcc ggcaagggca tcccggcggc
     6901 gctgttcatg ggatcgtcgc ggaacctgct gcgcgcggag gcgcggatca acaaccagcc
     6961 cggcatgttg ctgcattcgt cgaatcgcta catctacgag gactccgagc acgggatgtt
     7021 cgtgacgctc ttctacatgg tcgtggacat gcacaacagc ctcatcacct acgggagcgc
     7081 cggtcacaac gaccagctca tcatcaagcg aaccacgcgc gatgtggtgc gcctcaatgc
     7141 gcgcggcatg gcgctgggca tgcagccgga ctccgagtac gaggagaagg tgaccttctt
     7201 cgagccgggc gactgggcgg tcctgttcac cgacggcgtg ctggagtacc tcggcgagat
     7261 cgacatcgac gcgggcgagg cgcgcctcat cgacatcgcg atgcagtatc tcgatcgcgg
     7321 cccgcaggag ttcatcgact gcctgcgcgg catcatcgag gagacaaagg agcagagcga
     7381 cttcatcgac gatttcacca tcgtcgccat ccagctgtga tgcgccgcgc cgcctgcatc
     7441 atcatcctgc ttgccgttgc cgttcccctg ggttcgcaga tgccggactg gttcaccgtc
     7501 cgcgaccgcg acggaaacga atacttcatg gaccgcaacg ggaagatatg gacctcgggg
     7561 agccccgatt tttcctacaa gccggcgtcg atcgaggggc tcgattattt cgtgacgcag
     7621 ggcgttgatc tcatccggcg ccattatccg gccgaggggc tcacgatgct caagtcggtg
     7681 atggcgctgc cggtcagcaa caagaccatc tacgacgcgc aggtgaaggc gtcgcgcgcg
     7741 atccaggcgc tccagcggcg cgaggggacg cgctatacgg cgctcagcga gcgggcgtcg
     7801 ctcgtatact accgcgaggg cgaggccgtc acgctgcaca acgaccgcat gaactggacc
     7861 atccgctttc cccgccccgt gacggtgatt cgccgcagcg cgcggacgcg aaacggctac
     7921 ctgcgccagg gggtgctggt gggcgtcacc ttcgccccgc gcccgcccga ggccgcatcg
     7981 gaggcgccga aatacgacgc cctggtcgcg atcgacagcg agcagtttcc cttcgagctc
     8041 acgtcggtaa aaaaactgga ggcgcactgg tcaaacgccc tcggcggcgc ggagacggac
     8101 cggcacctgg accatcgggc gcccgaccgc gcggtgtacc attgccgcga caagcggccc
     8161 ggcgagtaca gctggtatga gggctattac gtgaacgggc gctttggcac catggtgcgc
     8221 atgatcatgg agaccggcgc ggagggtcgg cacggcaggg cgatgctgca ggcgctcaag
     8281 gacttcaaac actgaacctg cggattgtta cggcttcggt tccttctcgg tgcgcatccc
     8341 cttgagacgc tggtaggtct cgccgaacac ccggtccccg tcggcgcttt cctgggtgcg
     8401 gatggaggga tattcctcgt tgaatgcgtt gataaagcgc tcgatctcct tctcgctgtc
     8461 ggtgaacttc acgcccacct cgcgcccgtc gattcgcacc acctgcgcgt tcatgatcac
     8521 cttgcgggtc ttgaagggga agcgtacctg gatgaactcg cccttgttga tgatggtgtt
     8581 gcgcaggaag cacagccccc ccgtgctcag gttgatcacc tcgacggtcg tgggcgtctc
     8641 cgaacccttg attcggtagt cgactttgag gttcacggtc ttgagccggc cgtagtcgcg
     8701 cttgtcgatt gatctggttc gtctcatggc tgcatggtgc cacgggcgtt cgaaagcgtc
     8761 aacgccgttt cgtcgcaatt gtcctggtgg gcgcagtagc ggcagtcctt ccagatcttt
     8821 tccggcaggg tcgatttttc gaccgcgcaa aaccccaggc gggtgaaaaa cgccgggacg
     8881 gcggtaagca cgaagatctt cgcgtcgggg aaatcgcgcc gcaggcgctc cacgaggccc
     8941 gtgaccagaa gggtgccgat gcccccgcgc cgctcggtgt cagccaccgc gagggagcgg
     9001 acctccttga ggtgcgcgcc gtagtcgtgg cgcgatacga tgcccacgac cgtcccattg
     9061 cgatcggcga cgaggaagtg cggaagcgat ttgagcacgt cggcgcgatc gcggggcagg
     9121 atcacccccc gcacggcgta gtgattgatg atggcgagta ttgcgtcgac gtcttgg
//
LOCUS       DAORHF010000147         9156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7000_length_9156_cov_5.734205, whole genome shotgun sequence.
ACCESSION   DAORHF010000147 DAORHF010000000
VERSION     DAORHF010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7000_length_9156_cov_5.734205"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..758
                     /locus_tag="PKJ16_12155"
     CDS             420..758
                     /locus_tag="PKJ16_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47786.1"
                     /translation="MNRKYVITGPLGLPLGAVMTLDVRRIETNEKSQAAHVEIVRVNG
                     NALAKPVRMRYRMLIARDAMVVGATYRVKAYQDGSFAGTPSAVLTDVMFQTAEYRFEV
                     VLVVYRIEKI"
     gene            776..1741
                     /locus_tag="PKJ16_12160"
     CDS             776..1741
                     /locus_tag="PKJ16_12160"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV47787.1"
                     /translation="MSGSISRTAVRCWCAALSIAALLHAGAWNAHAAPLVVPPGARLA
                     LAGIQGTKVDASTAAFYFELVATEWRNTGVFTLIDPALSGRRLAAAGITIAHHGVEPK
                     DAVAAGKALGAQYILTGRVQAFAGKHHVFVKLYDATSGRLVLSQVCIGKHYKEMMAGI
                     HRFIKSGTPAAVIDEKAWKAGRGTVAAGRSAAVMDVDSSPGDRELARAIQMMLRDEVV
                     KSNLFAVVEREKLEKLVQEVELAQAGLLKGDARTPADGSSRLKAAGYMLFGSYARIDD
                     TGVFSFRVVNVGTGIVEYSDRAFGAKARELSGEIRRIVSDLKTKH"
     gene            complement(1863..2645)
                     /locus_tag="PKJ16_12165"
     CDS             complement(1863..2645)
                     /locus_tag="PKJ16_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47788.1"
                     /translation="MRIVRSNARVIVSAAVAVTIAVTIAFFIVRQRGRAIERENRQRQ
                     LEIDRAAHPAETALLERYAAIVAERLPDVSCALRPPTVIAIDGHSGIREIGVHLGPQS
                     RAVGTITIRTDPSFTAHGFKLFGEIFLQSPPSGGPTAEGARAPARPAVIAPDGSIVHD
                     DFTITFADYREPIQAAPAGARQPVRNPVFDPATGRIIPRDGARQPLIGDTEHAVNAGA
                     TITVKPPEVVAREITITMKTDRSGVARDVFVRSLQSLLGSKR"
     gene            2776..3105
                     /locus_tag="PKJ16_12170"
     CDS             2776..3105
                     /locus_tag="PKJ16_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47789.1"
                     /translation="MGFFKDASESLFTYSEKLITKTEEYARIAKLTMDIKRLEMSIGR
                     INAEVGEYVVKRADAGDASIALSDEFIVEKTRAIAGVREEIEAKRQEIAAIKSARPTD
                     RGGSGAQ"
     gene            3133..5229
                     /locus_tag="PKJ16_12175"
     CDS             3133..5229
                     /locus_tag="PKJ16_12175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HNV47790.1"
                     /translation="MPVSSKRIIAHLKALTEPVTPESLLQGLDEAKRRRQRTEVRKKK
                     GPGRSRKEVAKAASILNDLAAAGFLQKKRGKYVPASPFRLEGVLRLQRANRGVFVGVD
                     GQEVLVKGENLGRAHNDDYVLVDVVDCRHGEVHGVVRDVLRRSRELFFAKVTGKTRRG
                     VHLSVMDLPGGLTATAPRAAGEPDEGHYAIIRLDDHIEGGEHACAIVDAVLPDEESYD
                     FSRVVIKHALPEAYPAAEEYERLRERITRIDPRRKNYRELFTVTIDGEHAKDFDDAIS
                     LETVGGKTVLYVHIADVAHFVDRGSALDREALRRGNSYYLGRHVIPMLPEVLSNDLCS
                     LRAGEDRLAVTAEMEIGADGAIVASRFFEGVILVDHRLTYRSADELIGSDDGSDLSAR
                     LRAMHELALRLKKKRLADGRIDLNLPDEDVRYDGVNIADIEFATRYRSHAVVEEFMLS
                     ANEAVSKALRENDIPTLYRVHEPIADEKLEMLRRFLAVCDITLERRLRTGVALQKVVD
                     SVAGREYEQVVNFIVLKSMMQAYYDEKPLGHFGLGFADYTHFTSPIRRYPDLVVHRCL
                     KSLIFGAKPPYGVPELVSIGETASDTERTAMRAERDFVKIKACRLLASRVGQLFDCIV
                     SGVSKYGIYVTLIDKPIEGMVPLRTLTDDYYLVQEDDYMVVGKRLGRRFRLGDRVSCR
                     LIAVELEMMRIDFEMQ"
     gene            5229..5453
                     /locus_tag="PKJ16_12180"
     CDS             5229..5453
                     /locus_tag="PKJ16_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47791.1"
                     /translation="MMNRDTYRRFVDAHRAEIDAQAAALANFDPTSLGFMKIQLVQAI
                     NIRLRMPVVPMTELTKLLVNFFAVQEIEDR"
     gene            complement(5432..7126)
                     /locus_tag="PKJ16_12185"
     CDS             complement(5432..7126)
                     /locus_tag="PKJ16_12185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HNV47792.1"
                     /translation="MHNTLLLVAVNARYSHPNLALFSLRTCVADLGHRTVIREFTIND
                     SDDAIVRGIVAEEPAIVALSAYIWNARCLKRIIPRIGTALPHARIILGGPEASYTAHA
                     WLADFPRIDCIVAGHGETGFRRLVSGETDAGERIITIPNTPFDEIPFPYRDEDFADHN
                     LGVRNVYYESSRGCPFRCAYCLSSRSDQRLEFRDPERVFDEIDQLMRHHPRLIKFVDR
                     TFNARPERAIALWEGIAARYDGSGTRFHFEVHPALLDDETLRALAGIPAGLFQFELGI
                     QSAHDATIEAIGRSGRWERVRESIARLRESGNMHLHADMIAGLPGEDADAVRRSFNAI
                     YALGADHFQLGFLKVLPGTRLADEAALYGIAHDPEPPYAIIESARLSRDVIAGLHAID
                     RLVNALHNTGRFVTTLAALMEEFSDPFAAYRALADHARETAQHPPDRAWEPNAALILD
                     FAAAHLPHRRGYLRDCLAWDWCATTRTHVYPAMLKRAEAAQARKSFYKSIERGDPATP
                     VMIGDCALSWDELRAAAFFHAATDEFRARRMRGHPAALHLRAGRGGPRVVYLSSIS"
     gene            7339..8691
                     /gene="tig"
                     /locus_tag="PKJ16_12190"
     CDS             7339..8691
                     /gene="tig"
                     /locus_tag="PKJ16_12190"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HNV47793.1"
                     /translation="MQILEKKLENATYEFQITVPENTVEIEYKSVFDRIQKNARIDGF
                     RKGKAPLGLIEQRFRAEADAEVAENILRTVYGDALIERKLMPVGRPSFEFDGIKKGEP
                     FSFKVTFEVAPTVEIAPYTGLPVEQRASEITEKDVDREIETMREQNATIQKKEADGTV
                     ENGNLVKFLIKRIDNIDPAEVEAAEFKEYSIIVGKSKEEHTIDRDILGMKVDETREAK
                     VKYPKKYEVEDLAGQKVTYLVKIVEISTMTLPELNDEFAKDMGEYATYADLRKDVRDY
                     LTRFTEIKSKGEAKQKLLVDVIEKSSFEIPGTMIESEVGAVYRRMLQRMGMSAESVSL
                     EDFAKATGTEVSALKDQLRPEALKSIKSMIALLEIAKRENITVDPAKYREVVESLAKR
                     NNATVEQMEEMVEERQLRENIETEIIMESALELLYEKAQVKRLKAVPLTEFMKAGDKS
                     "
     gene            8851..>9156
                     /locus_tag="PKJ16_12195"
     CDS             8851..>9156
                     /locus_tag="PKJ16_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNV47794.1"
                     /translation="MSLVPIVVEQTSRGERSYDIYSRLLKDRIIFLGEEIDDHISSLV
                     IAQLLFLEAEDPDKDIFLYINSPGGVVTSGMAIYDTMQYIKPDVATICIGQAASMGSL
                     "
BASE COUNT         1796 a         2741 c         3039 g         1580 t
ORIGIN      
        1 tgtataatgc ggttgcgcaa ccggtgagag taaaagacga caatataatg gcaatagtga
       61 ataaggtcga aagtctcatg atggcctcca ttgctaagaa acttattata tcaaactgcc
      121 gtctgtcaag tttaatttga cataatggca tttcttgttt atgaaaatca cgcacttgga
      181 cccttgttca tgggtgtgtg ccgcttcggg tttgcgtatt gctgtttata caaggtctcg
      241 atgaaaaatg gatagactgg tttttcccga tggaatatat cgaattctcg aaccgcacac
      301 cgagaaggaa agggatgatg aaaagaatta ttaacattct gttcgtagcc gcagtgcttg
      361 tgtcgtttcg taattcacgg gcgataggtg aggatggaag gacgattcca tacaaggaag
      421 tgaaccgaaa gtatgtcatc accggtccgc ttggcctgcc gctcggcgcg gtgatgacgc
      481 tcgatgtccg ccgcatcgaa acgaacgaga agtcacaggc cgcgcacgtg gagattgtgc
      541 gcgtgaacgg gaacgcgctc gcgaagccgg tccgcatgcg ctatcgcatg ctcatcgcgc
      601 gtgatgccat ggtcgtcggc gcgacctatc gcgtgaaggc gtatcaggac ggctccttcg
      661 cggggacgcc gtcggcggtg ctcacggacg tcatgttcca gacggccgag taccgattcg
      721 aggtcgtgct ggtggtgtat cggatcgaga agatatgatc tggaggtgtg tggaaatgag
      781 tggatcgata tcgcgaacgg cggtgcgatg ctggtgtgcg gcgctgtcga ttgccgcatt
      841 gcttcacgcg ggtgcgtgga atgcgcatgc ggcgccgctg gtcgtccccc cgggggcgcg
      901 cctggctttg gcgggcatac aggggacgaa agtcgacgcg tcgaccgccg cgttctattt
      961 cgagctcgtc gccaccgaat ggcgaaacac cggcgtcttc acgctcatcg atcccgcgct
     1021 gtctgggcgg aggctggctg cggcggggat cacgatcgcg caccatggcg tggagccgaa
     1081 ggatgcggtg gccgcgggga aggcgctcgg cgcgcagtat atcctgacag ggcgcgttca
     1141 ggcctttgcg gggaagcatc atgtattcgt caagttgtac gatgcgacga gcgggcgcct
     1201 ggtcttgagc caggtgtgca tcggcaagca ttacaaggag atgatggccg ggatccaccg
     1261 cttcatcaaa tcggggaccc ctgcggcggt catcgacgag aaggcgtgga aggccgggcg
     1321 cggtacggtg gccgccgggc gcagcgccgc ggtgatggac gtggattcgt cgccgggcga
     1381 tcgtgagctc gcccgcgcca tccagatgat gctgcgcgac gaggtcgtga agtccaacct
     1441 gttcgccgtc gtggagcgcg aaaagctcga gaagctggtg caggaggtgg agctcgcaca
     1501 ggccgggctg ctcaagggcg acgcgcgcac cccggcggac ggatcgtcgc gtctcaaggc
     1561 ggcgggatac atgctgttcg gatcgtacgc gcggatagac gacaccggcg tattcagctt
     1621 tcgggtcgtc aacgtgggga ccggcatcgt cgagtacagc gaccgcgcct ttggcgcaaa
     1681 ggcgcgcgag ctcagcggcg agattcggcg catcgtgagc gatctcaaga cgaagcacta
     1741 gctccattcc ctctgaggtt tccttcgtct tcgtgatcgc attcgttttg cggcggttca
     1801 cttcggaaac acgacacgga atatagcggg ggctgatccg cgtcccgcgc cggatgcccg
     1861 catcagcgct tcgaccccag gagcgattgg agcgagcgga cgaacacgtc ccgggcgacg
     1921 ccgcttcggt ccgtcttcat cgtaatggtg atctcgcgcg cgaccacctc cggcggtttt
     1981 accgtaatgg tcgcgcctgc gttcacggcg tgttcggtgt cgccgatcag cggctggcgc
     2041 gcgccgtccc gggggatgat ccggccggta gcgggatcga acacgggatt gcgcacgggt
     2101 tgccgcgcgc ccgcgggcgc cgcctggatc ggttcccggt aatcggcgaa ggtgatggtg
     2161 aaatcgtcgt gtacgatcga tccgtcgggc gcgatcaccg ctggtcgcgc cggcgcgcga
     2221 gctccctcgg cggtcgggcc gccgctcggg ggcgactgga ggaatatctc cccgaagagc
     2281 ttgaagccgt gcgccgtgaa cgacgggtcc gtgcggatgg tgatcgtacc caccgcgcgc
     2341 gactgcggac cgagatggac gccgatctcc cgtatgcccg aatggccgtc gatcgcgatg
     2401 acggtgggcg ggcggagggc gcacgaaacg tcgggaaggc gctcggcgac gatcgcggcg
     2461 tatcgctcca gaagcgccgt ctcggcgggg tgggccgccc ggtcgatctc gagttgccgc
     2521 tgccggttct cccgctcgat cgcgcggccg cgctgtcgga cgatgaagaa tgcgatcgtc
     2581 acggcgatcg tgaccgcgac agcggcactg acgatcacgc gcgcgttcga gcgcacgatg
     2641 cgcatcaatc tctccgccgc gtccgccgcg cgcgtccggt gttcattggc gtccatggaa
     2701 taaaaatatc attattattg acaaaatcaa caaataattg catcgttcga accgagcact
     2761 catgtggagg aacttatggg cttttttaag gacgccagcg aatcgttgtt tacatatagt
     2821 gaaaaactca tcaccaagac cgaggagtac gcccgcatcg ccaagctcac catggacatc
     2881 aagcgactgg agatgagcat cggccggatc aacgccgagg tgggcgagta cgtcgtcaag
     2941 cgcgccgacg cgggcgatgc gagcatcgcc ctctccgatg aattcatcgt cgaaaagacc
     3001 cgcgccatcg cgggcgtccg cgaggagatc gaggcgaagc gccaggagat cgccgcgatc
     3061 aagagcgcgc gcccgaccga tcggggcggg agcggcgcgc agtaaccccg tcggggaccg
     3121 cgccgcacgc ctatgccggt atcatcgaaa cggatcatcg cccacctcaa ggccctcacc
     3181 gaaccggtca cgcccgagtc gctcctccag gggctcgatg aggccaagcg acggcgtcag
     3241 cgtactgagg tccggaaaaa gaaggggccc gggcgctcgc gcaaggaagt cgcgaaagcc
     3301 gcatccatcc tcaacgacct ggcagccgcc ggatttttac agaagaagcg cggaaagtac
     3361 gtccccgcat ccccgttccg tctcgagggg gtgctgcgcc ttcagcgggc gaatcgcggc
     3421 gttttcgtcg gcgtcgacgg ccaggaggtg ctcgtcaagg gggagaacct cggccgcgcg
     3481 cacaacgacg actacgtgct ggtcgatgtg gtcgactgca ggcacggcga ggtgcacggc
     3541 gtcgtgcgcg atgtcctgcg tcggagccgc gaactgtttt tcgcgaaggt caccgggaag
     3601 acgcgacgag gcgtccatct gtcggtcatg gacctcccgg gcgggctcac ggcgaccgcg
     3661 ccgcgcgctg cgggcgagcc ggacgaggga cactacgcca tcatacgcct cgacgatcat
     3721 atcgagggcg gcgagcacgc gtgcgcgatc gtcgacgcgg tcctgcccga cgaggagtcc
     3781 tacgatttct cccgcgtcgt catcaagcac gcgctgccgg aggcctaccc cgcggcggag
     3841 gaatacgagc gcctgcgcga gcgcatcacg cgcatcgacc cgcgccggaa aaactaccgg
     3901 gaacttttca cggtgaccat cgacggcgag cacgcgaagg atttcgacga cgcgatatcg
     3961 ctggaaacgg tcggcgggaa gacggtcctg tacgtccaca tcgccgacgt cgcgcacttc
     4021 gtggaccggg gaagcgcgct cgaccgcgag gcgctccggc gcggcaacag ctattatctg
     4081 ggacggcacg tgatccccat gctcccggag gtgctctcga acgacctgtg ctcgctgcgg
     4141 gccggcgagg accgtctcgc ggtcaccgcg gagatggaga tcggcgccga cggcgccatc
     4201 gtcgcgtcgc gcttcttcga gggcgtcatc ctcgtcgatc accgcctgac gtatcgcagc
     4261 gccgacgagc tgatcgggag cgacgacggc tccgatctct ccgcccggct gcgcgcgatg
     4321 cacgaactcg ccctccggct caagaagaag cgcctggccg atgggcgcat cgacctcaat
     4381 ctgcccgatg aggacgtgcg ctacgacggc gtgaacatcg cggacatcga gttcgcgacg
     4441 cgctaccgaa gccatgccgt cgtcgaggag ttcatgctct cggcgaacga ggcggtttcg
     4501 aaggcgttgc gcgaaaacga catcccgacc ctctaccgcg tccatgaacc catcgccgat
     4561 gaaaagctcg agatgcttcg gcgctttctc gccgtgtgcg atatcacgct cgagcggcgg
     4621 ttgcgcaccg gggtcgccct gcagaaggtc gtcgacagcg tggcgggcag ggagtacgag
     4681 caggtggtga atttcatcgt cctgaaatcg atgatgcagg cctactacga cgagaagccg
     4741 ttgggacact tcggactcgg cttcgccgac tacacgcatt tcacctcgcc catccgccgc
     4801 tatccggacc tcgtggtgca ccgctgcctc aagagcctca tcttcggcgc gaagccgcca
     4861 tacggagtgc cggagctcgt ttcgatcggc gagacggcgt cggacaccga gcgcacggcg
     4921 atgcgcgccg agcgcgattt cgtgaagatc aaggcgtgcc gccttctcgc gtcgcgggtg
     4981 gggcagctct tcgactgcat cgtgagcggc gtctcgaagt acgggatcta cgtcactctc
     5041 atcgacaagc ccatcgaggg gatggtgccg ctgcgcaccc tcaccgacga ctactacctg
     5101 gtccaggagg acgattacat ggtggtgggc aagcggctcg gccgtcgctt ccgcctcggc
     5161 gaccgggtct cctgccggct gatcgcggtt gagctcgaga tgatgcggat cgatttcgag
     5221 atgcagtgat gatgaaccgc gatacctatc ggcgcttcgt cgatgcgcac cgcgccgaga
     5281 tcgacgcgca ggcggcggcg cttgcgaact tcgatcccac ctcgctgggg ttcatgaaga
     5341 tccagctcgt tcaggccatc aatatccggc tgcgcatgcc cgtggtgcct atgacggagc
     5401 tcacgaaact gctggtgaat ttcttcgcgg ttcaggaaat cgaggacaga tagaccaccc
     5461 gcgggccgcc gcgcccggcg cgaaggtgca gcgcggcggg atgtccccgc atcctgcgcg
     5521 cgcggaactc gtcggtcgcg gcgtggaaga acgccgccgc gcgcagctcg tcccacgaca
     5581 gcgcgcaatc gccgatcatc acgggggtcg cgggatcgcc ccgttcaatc gatttataaa
     5641 aagattttcg tgcctgcgca gcctccgccc gcttgagcat ggccggatac acgtgggtgc
     5701 gcgtggtcgc gcaccagtcc caggcgagac aatcgcgaag atagccgcga cggtgcggga
     5761 gatgcgcggc cgcgaaatcg agaattagcg cggcgtttgg ctcccatgcc ctgtccggcg
     5821 ggtgttgcgc ggtttcgcgc gcgtggtccg caagggcgcg atatgccgcg aagggatcgg
     5881 aaaactcctc catgagggcg gcgagcgtcg tgacgaagcg gccggtattg tgcaatgcgt
     5941 ttacgaggcg gtcgatcgca tgcaggcccg cgatgacatc gcgagagagg cgcgcgctct
     6001 cgatgatggc gtacggcggc tccggatcgt gtgcgatgcc gtacaacgcc gcctcgtccg
     6061 cgagtcgcgt gccggggagc accttgagga atcccagctg gaagtggtcg gccccgagcg
     6121 cgtagatggc gttgaaggag cggcgcaccg catccgcgtc ctccccgggc agccccgcga
     6181 tcatgtccgc atgcagatgc atgttccccg actcgcgcag ccgcgcgatg ctctcgcgaa
     6241 cgcgctccca tcggccgctg cgcccgatcg cctcgatggt cgcgtcgtgt gctgattgta
     6301 tgcccagctc gaattggaag aggcccgcgg gaatgccggc gagcgcgcga agcgtctcgt
     6361 cgtcgagcag ggccgggtgc acctcgaagt ggaatcgcgt gcccgatccg tcgtagcgcg
     6421 cggcgatgcc ctcccagagc gcgatggcgc gctccgggcg cgcgttgaag gtccgatcga
     6481 cgaacttgat caaccggggg tgatgccgca taagctggtc gatctcgtcg aacacgcgct
     6541 ccgggtcccg aaactccagg cgctggtcgc ttcgcgacga gaggcagtag gcgcaccgga
     6601 acgggcaccc gcggctgctt tcgtagtaca cgttgcggac gcccagattg tggtccgcga
     6661 aatcctcgtc gcgataggga aacggaatct cgtcgaacgg cgtattggga atggtgataa
     6721 tgcgctcgcc cgcatccgtc tctccgctca cgaggcgccg gaatcccgtc tcgccgtgac
     6781 cggccacgat gcaatcgatg cgcgggaaat ccgcgagcca ggcatgcgcc gtgtaactgg
     6841 cctccgggcc gccgaggatg attcgcgcgt gcggaagcgc cgtcccgatg cgcgggatga
     6901 tgcgcttgag gcaccgcgcg ttccagatat acgccgagag cgcgacgatg gccggttcct
     6961 cggcgacaat tccccgcacg atcgcatcgt cgctgtcgtt gatggtgaac tcgcggatga
     7021 cggtccgatg gcccaggtcc gcgacgcagg tgcgcagcga gaagagcgcc aggttggggt
     7081 gcgaatagcg cgcgttaacg gcgacgagga gcaatgtgtt gtgcatggcg gcggtttcat
     7141 cgtgcgcatc ggcgcagggc gcacgacgcg cgaggcgagc atgtcacggc gtcgcgatca
     7201 tcgtcaaatg aaatatgcaa aaaggggccg gagtcatgcc gaacagaaaa atgtattgac
     7261 gccctgaggg gctctccgtt tacatgacat aaaatttgaa tcgtttagga agcgatcaac
     7321 gcggggggta gtagcgccgt gcagatattg gaaaagaagc tcgaaaacgc cacctatgaa
     7381 ttccagatca ccgttccgga aaacacggtg gagatcgagt acaagtcggt cttcgaccgg
     7441 atacagaaga acgcgcgcat cgacggcttc cggaagggca aggcgcccct ggggctcatc
     7501 gagcagcgct ttcgcgccga ggccgacgcc gaggtcgccg agaacatcct ccggacggtg
     7561 tacggcgacg cgctcatcga gcgcaagctc atgcccgtgg ggcgcccgtc cttcgagttc
     7621 gacggcatca agaaggggga gccgttctcc ttcaaggtga ccttcgaggt cgcgcccacc
     7681 gtggagatcg ctccgtacac gggtctcccc gtggagcagc gcgccagcga aatcaccgaa
     7741 aaggacgtcg atcgcgagat cgagacgatg cgcgaacaaa acgcgaccat ccagaagaag
     7801 gaagccgatg ggacggtgga gaacggcaat ctggtgaagt tcctcatcaa gcggatcgac
     7861 aacatcgatc ccgccgaggt ggaggctgcc gagttcaagg aatacagcat catcgtcggc
     7921 aagagcaaag aggagcacac catcgaccgc gacatcctgg ggatgaaggt cgatgagacg
     7981 cgcgaggcga aggtcaaata cccgaagaag tacgaggtcg aggacctcgc gggccaaaag
     8041 gtgacctacc tggtgaagat cgtcgagata agcactatga cgctgcccga gctcaacgac
     8101 gagttcgcga aggacatggg cgagtacgcg acctacgccg atctccgtaa ggacgtccgt
     8161 gactacctga cgcgcttcac cgagatcaag tcgaaggggg aggcgaagca gaagctcctc
     8221 gtcgacgtga tcgagaagag ctcgttcgag atcccgggca ccatgatcga aagcgaggtc
     8281 ggcgccgtct atcgccgcat gctgcagcgg atgggcatga gcgcggagag cgtgagcctc
     8341 gaggacttcg cgaaggcgac cgggaccgag gtgtccgcac tcaaggacca gcttcggccg
     8401 gaggcgctca agtccatcaa gtccatgatc gcgctcctgg agatcgccaa gcgggagaac
     8461 atcacggtcg atccggcgaa gtaccgcgag gtggtcgagt ccctggccaa gcgcaacaac
     8521 gccacggtgg agcagatgga agagatggtc gaggagcggc agctgcgcga gaacatcgag
     8581 accgagatca tcatggagtc ggcgctggag ctgctctacg agaaggcgca ggtgaagcgc
     8641 ctgaaggccg ttccgctcac cgagttcatg aaggccggcg acaaatccta acgcgacata
     8701 tttttgttgc cagcgcggcc ccgcggcgat atactaccct acaggatccc gcggtccgcg
     8761 aaaattattg gcagctcgcc tgccgcgctt gccgaaaagt atcctaggcg gccgcacgga
     8821 caaaccaata cacaatccaa ggagtgttac atgagccttg tgccaatcgt tgttgagcag
     8881 acgagccgcg gagagcgctc gtatgacatc tattcgcgcc tgctgaaaga ccgcatcata
     8941 tttctcggag aggagatcga cgaccacatc tcgagcctgg tgatcgccca gctcctcttt
     9001 ctcgaggccg aagatcccga caaggacatc ttcctctata ttaacagccc gggcggggtg
     9061 gtcacctcgg ggatggcaat ctacgacacc atgcagtaca taaagcccga cgtcgccacc
     9121 atctgcatcg gccaggcggc ctcgatgggt tcgctg
//
LOCUS       DAORHF010000148         9061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7096_length_9061_cov_5.562403, whole genome shotgun sequence.
ACCESSION   DAORHF010000148 DAORHF010000000
VERSION     DAORHF010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7096_length_9061_cov_5.562403"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PKJ16_12200"
     CDS             complement(<1..480)
                     /locus_tag="PKJ16_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HNV47795.1"
                     /translation="MSRDYEKTGLQISMAGSALLSLSAIVMALIIQSQAVLLDGLYTF
                     TTLIMSLISLKIVDLVNRPETRNRPFGYMALEPFSNLIKSLVMVVLLLVFLVTNIQEI
                     FTGGRVISLNLTMLYTAVCMVIYLVIIVLLKKCGRKSRSSILELEVRSWFIDTLLTAG
                     "
     gene            complement(669..1415)
                     /locus_tag="PKJ16_12205"
     CDS             complement(669..1415)
                     /locus_tag="PKJ16_12205"
                     /inference="COORDINATES: protein motif:HMM:NF023734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DA1"
                     /protein_id="HNV47796.1"
                     /translation="MEYLLNIAIHFRRRMLGKSPAIAPTICSVFLMGVLLVGLGNVVA
                     CQYFSPTCKFCGSSLSAGKVHTPEGHDVCKECNRDAVRDVRTAQALVAQVRSELASFG
                     IHLPWGAIPIKMGPSANPAVYARCEAMRYANGSVGALWIRFVPGLPKSMFKSTAAHEL
                     THAWAYLNRCPANQDEALSEGAPTLVEYLYLERYRSTYGERRRNEIMTSSNRIYGKGT
                     RRLQAYAKDHGGLPGVLTLLRTGQTIPKGY"
     gene            complement(1530..1715)
                     /locus_tag="PKJ16_12210"
     CDS             complement(1530..1715)
                     /locus_tag="PKJ16_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47797.1"
                     /translation="MAIYSTWLVRAITFGFATGITLFTFILVRKQARGDIVLLNHEQN
                     LRPMSHTLWPEEDSLQG"
     gene            complement(1727..2257)
                     /locus_tag="PKJ16_12215"
     CDS             complement(1727..2257)
                     /locus_tag="PKJ16_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47798.1"
                     /translation="MKTGRTLRTAIILSLAFMIAATAEYLFSNDREPPLILKLEIVQD
                     EHSKDSATVTERVSIIGRDVAYWWEGFGFRRDGRGNHRYRLSPDGYGSLVRYITTNRL
                     NRDLKEERPYGKIGLSISLRLNMIMNGVKTSAFLKGMYNEWGAKQERRCNLENIDFFR
                     RCKHIARQAKNKSDRF"
     gene            2758..3618
                     /locus_tag="PKJ16_12220"
     CDS             2758..3618
                     /locus_tag="PKJ16_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47799.1"
                     /translation="MDELLKNTDIIVPVADPAKIAKGTIVRRINTSKDQQGCFTNFDD
                     QGGMILVNVVDIKECAFVAEAGILRPSAEDSLYRFTTTFGNSPAMMDAIAIIQNWQVF
                     KKYPALQNAILNFVESTYTPEQLVYLSRREAMGCVFIPIQQKFRIGRFAEAIDWNKVG
                     RDKFRERLADMRPGDHVTYLAMIPQVKTGTPKFYSIGSKPHEETAICLRSEPFNFQPT
                     HGGHIKLLADFEGNEYFLVDAGSNFKGRGVKTPLSKAEEVTDSLAQLHGEFVFKPIEG
                     RGAFGEGQSY"
     gene            complement(3638..4270)
                     /locus_tag="PKJ16_12225"
     CDS             complement(3638..4270)
                     /locus_tag="PKJ16_12225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47800.1"
                     /translation="MTVRKHNGEGTFARLKKQEKDLRKQIIVDAAEKLFASKPFSRVS
                     MRDIAKEAGISPAAIYRHFPDQQALFLESFARGTSELKDEFDALMARPGGVTIEHAVE
                     RFIEYFSLKDQYFRMMINFFLEGTVAPDMFHKLSDIERAMLDQFDAIFSSLGARGDVR
                     FYSHALFAALTGIVAIFRSTPDKSDAQILEHRKRIARTVAGIFKDALAAR"
     gene            complement(4341..5150)
                     /locus_tag="PKJ16_12230"
     CDS             complement(4341..5150)
                     /locus_tag="PKJ16_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47801.1"
                     /translation="MNQDQVKSQLLRLNKDVEDFTVVFSGKKSRKVDGLYKPDTREIL
                     IHNKNFEGDNALMYTAIHEFAHHIQFTQAAAPSSTRVHTARFWDILHRLLYDAEHKGI
                     YANLFESDPRFTALTKRIREKYLGPGGALMKEFGRLLMEAEELCRECQASFDDYVDRE
                     LMVHRAAAKTIMKLHALDVNPTIGYENMKTVAKIRDPERRTEVQNMFLDGNSPDMVKA
                     RFMSNAEAADDPVQRLLHEKSKIMNAIKTLQERLLEIDERLHAMKGTSESA"
     gene            5188..5433
                     /locus_tag="PKJ16_12235"
     CDS             5188..5433
                     /locus_tag="PKJ16_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47802.1"
                     /translation="MSDAARAVAAAPSHPIEAVRPMWRLFPNLTHPTRIILIALMLVA
                     GGYFLLNGETALGIATLVGGVFFAVVYITNRHRGEGS"
     gene            complement(5448..6062)
                     /locus_tag="PKJ16_12240"
     CDS             complement(5448..6062)
                     /locus_tag="PKJ16_12240"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012738342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNV47803.1"
                     /translation="MRRAEPPAEKLVLKPGNMLYPVPAVLVTCRRGAEDNILTISWTG
                     TVCTNPPMLSISVRPERHSYPMLRETGVFAVNLPTRALARAVDFCGVTSGRDIDKFET
                     LRLTKVPASAIDAPLVRECPVSIECRVRQVIELGSHHLFLASVEAVDVDRSLMDAKGK
                     LRLEHADLLCYNHGSYCVASRPFGKFGFSVEKKKAKRPARNRTR"
     gene            complement(6071..7360)
                     /locus_tag="PKJ16_12245"
     CDS             complement(6071..7360)
                     /locus_tag="PKJ16_12245"
                     /inference="COORDINATES: protein motif:HMM:NF021354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HNV47804.1"
                     /translation="MDRFLHYTGGSGSLRRCFLRRSYGLFALALLFALGLVYDPRLVD
                     AQTATAPQRIIRVGVYGDGGVGSVPGGEREYAKVIADIINALNTRMRARVVTGEDIRG
                     GALKRMDVVIFPGGTGLGQYNSLGQTGRHQVQKFVYKGGGYIGICAGAYLAQTYEAKR
                     HGSSLQIARAKVFNKSRHWARGAGVIRVRMTNVGKRLLPELRNHTTFFMQYYNGPLLV
                     PLRGPKIRGYDELMRFESNVYHGDPRGKGESPGKTFLLRTRRGRGTVVLMSGHPESTP
                     GFRWLVPRMVEIAARVRPSRYPARFVRPNKYDREIMFTGAWFQEERRLIGIVKNADLE
                     EEKDVIAAIARLREMGSTDVCKHFPVLLGTDLPSVRIATAKAIVYFDYFPALGYLKKF
                     ISSEDDRAARAALIEARNYLSVSRPDRERVVIKEKAL"
     gene            7611..8564
                     /locus_tag="PKJ16_12250"
     CDS             7611..8564
                     /locus_tag="PKJ16_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47805.1"
                     /translation="MSNNVRIIVLSLLCFAFAGASITEAGEWSKEFGNGKFGNIMGVK
                     YDFKVGKTSEKSWAHSDAYLGTKMFGVNIPLIGADLIAGNIVGVSATAPSQTQQEIGI
                     DYDLAPFKNWKIHASIYYFGITIYSYDRNIDYTESTNDENLFTISKYVKKQMPRISWP
                     VGPVNFEIRYGASLTSSVSPKAKYAKEMVVNNNKRDMKGLEIELAPTISAKAFVEGGV
                     DAHVLRGGVGASLTIFEGWAGLVGYLDIIGNAALVKTKVGIEALKGRIYAYVDRRSVS
                     CSWKKGCRISWSRWINHTITEWKGFVYNLKDYILYSDGAAL"
     gene            8739..>9061
                     /locus_tag="PKJ16_12255"
     CDS             8739..>9061
                     /locus_tag="PKJ16_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47806.1"
                     /translation="MKFKFTRSPITSISIVAGSVAIIAALVSFSGSGDRHDSGNTADN
                     NAAINAGSSGPGTMVDLKYRLVPEKAYEFSFRREVMGSLKGETVIDVRLGGTLTAHVV
                     AADTDA"
BASE COUNT         1815 a         2641 c         2686 g         1919 t
ORIGIN      
        1 ccccgcggtc agcagggtgt cgatgaacca gcttcggacc tccagttcga ggatgctcga
       61 cctggatttt cgcccgcatt tcttcaggag tacgatgatg accaggtaga tgaccatgca
      121 caccgcggta tacagcatgg tcaggttgag cgagatcacc ctgcccccgg tgaagatctc
      181 ctgtatgttc gtaaccagga acacgaggag caggaccacc atcaccaggc tcttgatgag
      241 attggaaaac ggctccagcg ccatgtaccc gaacggccgg ttcctcgttt ccggcctgtt
      301 gaccaggtcg acgatcttca gggaaatgag cgacatgatg agcgtagtaa acgtgtacag
      361 cccgtccagc agcaccgcct gcgactgtat gatcagcgcc atcacgatgg cgctcagcga
      421 aagcagggcg cttcccgcca tcgagatctg caaaccggtt ttttcgtaat ccctgctcat
      481 gaatggcatg ccagcgaact ggccggatca tatgatacgg cgcgcgggac acctgtcaat
      541 cgcaatcgct gcgtgaaacc ttgattgaag ttttctgaaa taatttcgcc atgctactgc
      601 ccgacctcgt gtcggcggga cgaacgggga cggagataaa gagttacggt ggatgctgcc
      661 gcagccggtc agtagccctt ggggatcgtt tggccggttc tcaacaaggt caggaccccg
      721 ggaaggccgc cgtggtcttt ggcatacgct tgcaggcggc gcgtgccttt gccgtagatt
      781 ctgttgctgc ttgtcatgat ctcgttgcgc cggcgctcgc catacgtcga tcggtagcgc
      841 tcgagataga ggtactccac caaggtgggc gcaccctcgc tgagagcctc gtcctgattc
      901 gccgggcagc ggttcagata cgcccaggca tgcgtcagct catgggcggc ggttgatttg
      961 aacattgatt tgggcagccc cggcacgaac cgaatccaca gggcccccac gctcccgttg
     1021 gcgtaccgca tcgcctcaca acgggcgtac accgcagggt ttgcggacgg gcccatcttg
     1081 atcgggatcg cgccccatgg gagatgaatg ccaaagctgg caagctctga acggacctga
     1141 gcgaccaatg cctgggccgt acggacgtcg cgcacggcgt cccgattgca ctctttgcag
     1201 acatcgtgtc cctccggggt atgcaccttc ccagccgaga gcgacgaacc gcagaacttg
     1261 cacgtgggag agaaatactg gcatgccacc acgttgccca ggcccaccaa caggacaccc
     1321 atcaggaaaa ccgaacaaat agtaggcgca attgcgggtg acttgcccag catgcgtcga
     1381 cgaaaatgga tcgcgatgtt gagtaaatat tccattgaaa cgagcataaa ggataggagg
     1441 cagaatgtca acaacataac ataactactt aagaaacttc gcccctttcc gcaatgcgtc
     1501 aagaaacata ctgccggttg agaactggat cagccctgca gactgtcctc ttccggccat
     1561 aaagtgtgtg acatcggcct taaattttgc tcgtgattca ggaggacaat atcgccccgc
     1621 gcctgcttcc tcacgagtat gaaagtgaaa agcgtgatcc ccgtcgcgaa accgaaagtg
     1681 atcgcgcgca ccagccatgt actgtagatg gccatgccgc tccccctcag aaccggtccg
     1741 atttattctt cgcctgccgg gcgatgtgct tgcatcgccg gaagaagtcg atgttctcca
     1801 ggttgcatcg ccgttcctgc ttcgctcccc actcgttgta catgcccttc aggaacgccg
     1861 atgtcttcac tccgttcatt atcatgttga gcctcagcga gatcgacagc ccgatcttcc
     1921 cgtacggccg ctcctccttc aggtctcgat tgagccggtt cgtcgtgatg taccgcacca
     1981 ggctcccgta tccgtccggg ctcaaccggt agcggtgatt gccgcggccg tcgcgacgaa
     2041 agccgaaacc ctcccaccaa tacgcgacat ctcgcccgat gatgctcacc cgctcggtaa
     2101 cggtggcgct gtccttgctg tgctcgtctt ggacgatctc gagcttcagg atcagtgggg
     2161 gttctcggtc attcgaaaac aggtattcgg cagtcgcggc gatcatgaac gcaagggaga
     2221 gaatgattgc tgtccgcagg gtcctgccgg ttttcatgtg cgggttcctc ggggggatag
     2281 ttatcagtag acagttttct acttacgaaa ctccgcccct ttctgcaatg cgtcaagaga
     2341 catactgccg gttataagaa ctggatcatc cctgcagaca gcccttttcc ggccaaaaag
     2401 tgtgtgacat cggccttata tgtgcgcgga cgaggaagaa ttgaaaaatt ttcgccgcga
     2461 tgcgcgtgtg gtcccatgcc gcctcgacca ttcgaatcta gcagcttcca agggaaaagt
     2521 caacatgttt taacccctga aaacagggtt cagtctgcag gaacaaggca ttgcattgtc
     2581 cctacaggcg tgtgctcctg tagttcgcgc tcgcgttttt cctaatcagt ttttctcata
     2641 ccgcccatcc cgccgcgcga gagtcatttt tccttgacag ccgctgcgac gcggtgaact
     2701 gttttctacg aacggcgccg ctgcgttgca tcgcgtcttg aacgggagag aatccgcatg
     2761 gacgaactgc tgaagaacac cgacatcatc gtgcccgtgg ccgatccggc gaagatcgca
     2821 aaggggacga tcgtccgtcg catcaatacc agcaaggacc agcaggggtg ctttaccaat
     2881 ttcgacgacc agggagggat gatcctcgtc aatgtcgtcg acatcaagga gtgcgccttc
     2941 gtcgccgagg cggggatcct gcggccctcg gcggaggact cgctctatcg gttcacgacc
     3001 accttcggca acagccccgc catgatggac gcgatcgcca tcatccaaaa ctggcaggtg
     3061 ttcaagaagt acccggcgct ccagaacgcc atcctgaatt tcgtcgagtc gacctacacc
     3121 ccggagcagc tcgtatacct gagcaggcgc gaggcgatgg gctgcgtgtt cattcccata
     3181 cagcagaagt tccgcatcgg ccggttcgcg gaggccatcg actggaacaa ggtcgggcgc
     3241 gacaagttca gggagcggct cgcggacatg cggccgggcg atcacgtgac ctatctggcg
     3301 atgatcccgc aggtgaaaac gggcaccccc aagttctatt ccatcggcag caagccgcac
     3361 gaggagaccg ccatctgcct gcggagcgag ccgttcaatt tccagcccac ccacggcggg
     3421 cacatcaagc tcctggccga tttcgagggg aacgagtatt ttctggtgga cgcgggatcg
     3481 aacttcaagg gccgcggcgt gaagacgccg ctctcgaagg cggaggaggt gaccgactcg
     3541 ctcgcgcagc tccacgggga gttcgtgttc aagcccatcg agggccgcgg cgctttcggg
     3601 gaggggcaga gctactaggc cggcggcgcg acgcggatca ccgcgcggcg agggcgtcct
     3661 tgaagatgcc cgcgacggtc cgcgcgatac gcttgcggtg ctccaggatc tgcgcatcgc
     3721 tcttgtccgg cgtggagcgg aagatggcca cgatcccggt aagcgccgca aagagcgcat
     3781 gggagtagaa ccggacgtcc ccgcgtgcgc ccagcgagct aaagatcgcg tcgaactggt
     3841 ccagcatcgc gcgctcgatg tcggagagct tgtggaacat gtccggagcg accgtcccct
     3901 cgagaaaaaa gttgatcatc atccggaagt actggtcctt gagggagaag tactcgatga
     3961 agcgctccac cgcatgctcg atggtcaccc cgccggggcg ggccatgagc gcgtcgaact
     4021 cgtccttgag ctcgctggtg ccgcgcgcga acgactccag gaagagcgcc tgctggtcgg
     4081 gaaaatgccg gtagatggcc gcgggcgaga tccccgcctc cttcgcgatg tcgcgcatgc
     4141 ttacgcgcga gaagggcttg gacgcgaaga gcttctccgc ggcgtccacg atgatctgct
     4201 tgcgcaggtc cttctcctgt tttttgaggc gcgcgaaggt cccctcgccg ttgtgtttcc
     4261 gtaccgtcat tcctgtccag ggcgcgtgtg attggcaatg tgccgtgatc gtatacgata
     4321 gccccgatag cggattaatt tcaagcactt tcgctcgttc ccttcatcgc gtgcaaccgc
     4381 tcgtcgatct ccagcagacg ctcctgcagc gtcttgatgg cgttcatgat cttgctcttc
     4441 tcgtgcagga gccgctggac gggatcgtcg gcggcctcgg cgtttgacat gaagcgcgct
     4501 ttcaccatgt cgggactgtt cccgtccaga aacatgttct ggacctccgt gcggcgctcg
     4561 gggtcgcgta tcttcgcgac ggtcttcatg ttctcgtagc cgatcgtcgg gttcacgtcc
     4621 aacgcgtgca gcttcatgat cgtcttggcc gcggcgcggt ggaccatgag ctcgcggtcc
     4681 acgtaatcgt cgaagctcgc ctggcattcc cggcacaatt cctcggcctc catcagcagc
     4741 cggccgaatt ccttcatgag cgccccgccc gggccgaggt acttctcgcg gatccgtttc
     4801 gtgagcgccg tgaaacgagg gtcgctctcg aagaggttgg cgtagattcc cttgtgctcg
     4861 gcgtcgtaga gcaggcgatg caggatgtcc cagaagcgcg cggtgtgcac gcgcgtcgac
     4921 gacggcgcgg ccgcctgcgt gaactggatg tggtgcgcga actcgtggat cgccgtgtac
     4981 atgagcgcgt tgtcgccctc gaagtttttg ttgtggataa ggatctcgcg cgtatctggc
     5041 ttgtagagcc cgtccacctt gcggctcttt ttcccggaaa agacgaccgt gaagtcctca
     5101 acgtccttgt tcaggcgaag cagctgcgac ttgacctggt cctggttcat gcttttttcc
     5161 tttacgcgat tctcggggac ggccatcgtg tctgatgcgg cacgcgccgt cgcggcggcg
     5221 ccatcacacc cgatcgaggc ggtgcgaccc atgtggcgat tgttcccgaa cctgacgcat
     5281 ccaacaagaa taattctcat cgcgctgatg ctggtcgcgg gcggctactt tctcctcaac
     5341 ggggaaacgg cgctcgggat cgcgacgctc gtcggcggcg tcttctttgc cgtcgtctat
     5401 atcaccaacc gccaccgcgg cgaggggtca tagggcgcgc gcacggccta ccgcgtccgg
     5461 tttcgcgccg ggcgcttcgc tttctttttt tccacggaaa acccgaactt gccaaacggc
     5521 cgcgaggcca cgcagtagct gccgtgattg tagcacaaca ggtcggcgtg ctcgaggcga
     5581 agctttccct tcgcgtccat gagggagcgg tccacgtcca ccgcctccac cgaggcgaga
     5641 aagagatgat ggctccccag ctcgatcacc tgccggacgc ggcactcgat cgagacggga
     5701 cactcgcgca cgagcggcgc gtcgatcgcg ctcgcgggga ccttcgtgag gcgcagcgtt
     5761 tcaaacttgt cgatgtcgcg tccggaggtc acgccgcaga agtccaccgc gcgggcgagg
     5821 gcgcgggtcg gcaggttcac ggcgaacaca ccggtctcgc gcagcatggg gtacgaatgg
     5881 cgctcgggtc gcaccgagat ggacagcatg ggcgggttgg tgcacaccgt tccggtccac
     5941 gagatggtga ggatattgtc ctcggcgccg cggcggcagg tgaccagcac agccgggacc
     6001 ggatagagca tgttgccggg cttgaggacg agtttttcgg ctggcggttc ggcgcgtcgc
     6061 atgtcgtatc ctacaatgcc ttttccttga tgacgacccg ttcccgatcc gggcgcgaga
     6121 cggagagata gttgcgcgcc tcgatgagcg cagcccgggc ggcgcggtcg tcctcggacg
     6181 agatgaattt tttcaagtat cccagcgccg ggaagtaatc gaagtatacg atggccttcg
     6241 ccgtggcgat gcgaacggag ggcaggtcgg tccccagaag cacgggaaaa tgcttgcaca
     6301 cgtcggtcga tcccatctcg cggagccgcg cgatcgccgc gatcacgtcc ttttcctcct
     6361 cgaggtcggc gtttttcacg atcccgatga gccggcgctc ctcctggaac caggccccgg
     6421 tgaacatgat ttcgcgatcg tacttgttcg gacggacgaa ccgcgcgggg tagcgcgacg
     6481 ggcgcacgcg cgcggcgatc tccaccatgc gcggcacgag ccagcgaaag cccggtgtcg
     6541 attccgggtg tcccgacatg agcaccaccg tcccgcggcc gcgccgcgtc cgcaggagaa
     6601 aggtcttccc gggcgattcg cccttccccc ggggatcgcc atgatacacg ttggactcga
     6661 agcgcatgag ctcgtcgtat ccgcggattt taggaccccg gagcggaacc aggagcggcc
     6721 cgttatagta ttgcatgaag aacgtggtgt ggttcctgag ctcgggcagc aggcgttttc
     6781 ccacattggt catgcgcacg cggatcaccc ctgcgccgcg cgcccagtgc ctggatttgt
     6841 tgaagacctt cgcgcgggcg atctgcaggc tcgatccgtg ccgcttcgcc tcataggttt
     6901 gcgcaagata cgcgcccgcg caaatgccga tgtagccgcc gcccttgtag acgaactttt
     6961 gcacttggtg gcgccccgtt tgcccgaggc tgttatactg ccccaggccg gtcccgccgg
     7021 ggaagatgac cacgtccatg cgcttgagcg cgcccccgcg gatgtcctcg ccggtgacga
     7081 cccgcgcgcg catgcgcgtg ttcagggcgt tgatgatgtc ggcgatgacc ttcgcgtatt
     7141 cgcgctcgcc gccgggcacg ctccccacgc cgccgtcgcc gtaaaccccc acccggatga
     7201 tccgctgggg cgcagtggcc gtctgcgcat cgaccagtcg cggatcgtac acgagtccca
     7261 gtgcgaacag gagcgcgagt gcgaaaagtc cgtacgaacg ccgtaggaag catcgacgca
     7321 ggctgccgga tcctcctgta taatgaagaa atcggtccat gcgtactatt attgttatgt
     7381 cgcatattcc tcaagtagaa ttcgatgact ctgttcattt ttttatcact cgtcagtttc
     7441 tcccggaagg cgttgtgtta tgtttttccc accgtcctgc cggtttggaa catttttgga
     7501 ttaaaattct tgaccaaccg gccaatattt tttgcttctg tatcatctaa cggcgggctg
     7561 ggaccgctta tacgtacata tcatttttat taacagctca aggaggggta atgagtaaca
     7621 acgtacggat catcgtcttg tcattgttat gcttcgcgtt tgcaggtgct tcaattacgg
     7681 aggccggcga atggtccaag gaatttggca acggcaagtt cggcaacatc atgggcgtga
     7741 agtacgattt caaggtcggt aaaaccagtg aaaagtcatg ggcgcattcc gacgcatatc
     7801 tcgggacaaa gatgttcgga gtaaatattc cgcttattgg tgcggatctt attgcgggca
     7861 atatcgtggg ggtatcggct actgcaccca gtcagacgca acaggaaatc gggattgatt
     7921 atgacttggc gccctttaag aactggaaaa tacatgcgtc gatttattat ttcggcatca
     7981 ccatatattc ctatgatcga aacatagact acacggaatc aacgaatgat gaaaatctct
     8041 ttacgatttc gaaatacgta aaaaaacaaa tgccaaggat tagttggccc gttggtccgg
     8101 tgaactttga aattcgctat ggggcatcgc taacttcgag cgtgtcgcca aaagccaagt
     8161 atgcgaaaga aatggtcgtc aataataaca agcgggacat gaagggcctc gaaatcgaat
     8221 tggcgcccac catttccgcg aaagcgtttg tcgagggcgg cgttgatgcc catgtcttac
     8281 gcggcggcgt tggagcaagc ctcacgatct tcgagggctg ggccggcctg gtgggctatc
     8341 tcgatattat cggcaatgcc gcgctggtta aaacgaaggt ggggatcgag gcgctgaagg
     8401 gcagaatcta tgcgtatgtg gaccgtcgaa gcgtttcgtg cagctggaaa aagggatgtc
     8461 gcatatcctg gagccgttgg attaatcaca ccatcaccga atggaaaggg tttgtgtaca
     8521 atcttaaaga ctacatactc tattctgatg gcgctgcgct gtaggaactg cacgacgagg
     8581 taatggcaaa ccaggagagg ccggcctttt agagaggcgg cctctcccat tatcatgatt
     8641 caagtatctc ggattgggca gccaatgaat tctacgcacc gggatgcggc ccaacggcgc
     8701 tgttatcata ggacgcattg aaattttcgg gaaccgatat gaagttcaaa tttactcgtt
     8761 caccaatcac ctccatttct attgtcgcgg gttcggtcgc gatcattgcc gcgcttgtct
     8821 cgttttccgg cagcggcgat cgtcacgatt cggggaatac cgcggataac aacgctgcga
     8881 tcaacgcagg atcatccggt ccgggaacaa tggtcgattt gaaatatcga ttggtccccg
     8941 aaaaggccta tgagttttca ttcaggcgcg aggtgatggg ttcgctcaaa ggcgagaccg
     9001 tcatcgacgt ccgtctcggg ggtacgctga cggcccacgt ggtggccgcc gataccgacg
     9061 c
//
LOCUS       DAORHF010000149         9060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7099_length_9060_cov_4.344253, whole genome shotgun sequence.
ACCESSION   DAORHF010000149 DAORHF010000000
VERSION     DAORHF010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_9060_cov_4.344253"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PKJ16_12260"
     CDS             <1..642
                     /locus_tag="PKJ16_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV47807.1"
                     /translation="SVILFDEIEKAHHDIFNVLLQVLEEGELTDSQGQTVSFRDTIII
                     MTSNVGNEQYHRGGRMGFISEGQAVDAAREGVNDELKEVFRPEFLNRIDEVVHFHKLD
                     KKHMRRIVEIMLDDVRERLRDMGVDIVFSSSAKRLLAERGFDDKYGARFLRRTVQNEI
                     EDALALDLLKGSGRECALITVTVRKGRFHFAASAPQPSATADAETACEKANIP"
     gene            618..1886
                     /gene="cls"
                     /locus_tag="PKJ16_12265"
     CDS             618..1886
                     /gene="cls"
                     /locus_tag="PKJ16_12265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HNV47808.1"
                     /translation="MRKGKYPVTGKRRRERRPKRSDRRALSLINRRLKKLTARFFLPR
                     FLREMKRLKIGGLTGGNRLKLLADGDSCFREIIATIRKAKKSINLETYIFNSDALGWK
                     IAELLMEKSRAGVEVNCIYDAIGCIGTSPRLFSALREAGVEVLEYNPFFPWRRYWNLS
                     SRDHRKIIVVDGEIAFIGGINIGDDYAGRHFDGGNWRDTHLKVQGPAVRDIQFFFLEN
                     WYRQGGAIIDNGRHFPHIKEAGKKLLMVISDKARRKVRPIRESYISAIENAHHSIYIT
                     NAYFIPDGRIYRALIRAARRGVDVRIMLPEKSDVPIVQFASRYLYKRYLKSGVRVYEY
                     TRSVLHAKTAVIDGIWSTVGSSNIDRRSFRKNLEINAIILDQEFGEIMERQFDEDLRH
                     CVEKRLDEWQRRSAMLFALEWIMYRFRNYL"
     gene            complement(1893..2711)
                     /locus_tag="PKJ16_12270"
     CDS             complement(1893..2711)
                     /locus_tag="PKJ16_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HNV47809.1"
                     /translation="MNLEDFFPSKHIIFLKSNEKQLVIKEMLQKLHKLEIIENVDRYY
                     AQVIHRESLENTGIGKGFAIPHVRTDTVSNLIVMFGICRAGIEYNAFDQQPVKYLLLS
                     LFPTDMSTKYLYLVGMMASIFSEKSKTKLLDSATTAAKVYSALNTEGRKYLEVVSSKD
                     RIKKAVSAIPSGVPSSNLDLIIRLDRLYQITDGKKKNEASAKKIAELQKLIDNRSLTY
                     YERMRQKCQNPFSIVDNNSCSGCHMVIPAADANRMKRADKISLCPHCGRFLIFI"
     gene            complement(2746..2820)
                     /locus_tag="PKJ16_12275"
     tRNA            complement(2746..2820)
                     /locus_tag="PKJ16_12275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2783..2785),aa:Pro,seq:ggg)
     gene            complement(2874..4331)
                     /locus_tag="PKJ16_12280"
     CDS             complement(2874..4331)
                     /locus_tag="PKJ16_12280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV47810.1"
                     /translation="MDDRRDRIIAYAFLVIFVLTHVPYLLTPITGSHRLRQADTAAVA
                     RNFAVESPNILYPRVDRRGETSGITGMEFPLYQYVASIIHRITGSLHDGWGKLLSLVS
                     ALGVFLLLGSLLCRFFGIERRYSYAALFFVPAFFLFAGKYMPSITGLLFGVGGMYATL
                     QYHDDPKARHLVIAAMCLACAALIRPFLIFFCLPLLVIFIYSLAFQRRFRFGVLLTGV
                     SIIIPFVAWYYLWAPHLVRSYGLNIFFFGYSLSQTLAEMASVGLWIEFFKTVFQYYTG
                     YALIPVAIAGIVGFRRGLRANSPDQDETAHRPLPGYLAHTVVWIIPLATALTLFVIIG
                     RHFSPHYYYFFAFIPSAAILIAYGLRNAHELMRNYYYALLVVLGAIFFIAIGYTYRVD
                     DDVKSLVRLQPLVAEKTAPFDRVVIINTGTPPLYLHLVRRHGWTLFENELADPKTIRE
                     LTERGARWVITRENDTFALYAIDEWLSRISRKTAK"
     gene            complement(4346..4972)
                     /locus_tag="PKJ16_12285"
     CDS             complement(4346..4972)
                     /locus_tag="PKJ16_12285"
                     /inference="COORDINATES: protein motif:HMM:NF002305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosylase/DNA lyase"
                     /protein_id="HNV47811.1"
                     /translation="MRSIYDSIRVEVSRQLELFSRRWEFATDRELFIQLVFCLLTPQS
                     KARQCWRAVQILLEDDLLFHGRPADIAARIPIVRFRNKKAEYIVRARETLYGLDRPSL
                     RSILDQHAVAFDMRSWLASAVTGYGYKEASHFLRNIGHGGSLAILDRHILKNLTAFGV
                     IDGPPSNLSAARYLTYENAMRDFSQSINIPLDYLDFVLWYKETGDIFQ"
     gene            complement(5093..5398)
                     /locus_tag="PKJ16_12290"
     CDS             complement(5093..5398)
                     /locus_tag="PKJ16_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47812.1"
                     /translation="MDEPARFSMQQTLVFMCRDLIEDISDMRSFENDRDALEETIAIA
                     LSLTSYDRETLASLGRIIDRMATKTYTLFAKHYDDPVLDSLLKRILSLKDICSNLLE"
     gene            complement(5414..5938)
                     /locus_tag="PKJ16_12295"
     CDS             complement(5414..5938)
                     /locus_tag="PKJ16_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV47813.1"
                     /translation="MLLRTVTSGNGEIAHTLKKVSFLRMFADDDDIIKKISMICRKRR
                     FARGKLIIREGEYGDELYIVLSGKIEIVKKTMQNEKYTVTTLDAVLGGVYVGELALID
                     NDMRSATVIAKTDCICLAISRKDFIAFGNANPEIGLAITREIARQICGKLRKATTDII
                     TLFSALVEEIAITG"
     gene            complement(5938..7173)
                     /locus_tag="PKJ16_12300"
     CDS             complement(5938..7173)
                     /locus_tag="PKJ16_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HNV47814.1"
                     /translation="MKQLRELKYIIIRIFNTVIFKFLTAGIIVMILCSFGVYYMERDN
                     VAYKIEQGNRVEDNDKSSNIRTIEDSIWWAIVTSTTVGYGDMYPKTHAGRLFGIVTML
                     FGILLVGFITGNISSILVEKQLKEGRGLKGLRLKNHFIICGWKRDMGKFLRDILAKNK
                     NFLPSEIVLINTADPEQVESLRADADLTAIHYIHGDHIDEKILNRAALRHASKILVLA
                     DRMVTGNVQEVDSRTVMSIITIKSISKSIYTCAELLDSKFERYLRFSNCDEIVLTSDY
                     NRSVIANASCGNGISHIVGELLNVDASVSINTIDIPGEFVGKTYEELFQHVRKRDNSI
                     LIGILENTGNFYDRKTEALREAQKTPDISTLVDNLKLVKNLIPNNPILNPHPEYIIGK
                     HSRAIIIEGHDIHPAQAAS"
     gene            complement(7295..8497)
                     /locus_tag="PKJ16_12305"
     CDS             complement(7295..8497)
                     /locus_tag="PKJ16_12305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV47815.1"
                     /translation="MRDVYIVSAVRTPGCRRAKGAFAQTRPESLLAHILDAAVKKIKF
                     DVSAIDDVMIGCAFPEAEQGLNIGRIAAQMAGFPDSVSGAVVNRFCASGLESIAISAM
                     RIAMGWSEVTMAGGLESMSVVPMGGNMPRPHPDWSRKNPDVYISMGITAENVANRYKI
                     TREMQDEFAYHSNMKAAEAQEKGFFTEIVPTPAEKFVLKDGKWEKETFMQSIDDGVRK
                     ETTIDGLKKLKPAFSSTGSVTAGNSSQTTDGAAASVLMSEEAVKKYGVKPLVKFKMYT
                     VAGCKADEMGVGPAYAVPKLLKMAGLKASDIGIWELNEAFASQAIYSAQQIGIDDKKN
                     WAADSASRCINVSGGAIALGHPLGCTGSKLAATIVANMQRLGKKYGVESMCIGGGMGA
                     AALFELCD"
     gene            complement(8522..>9060)
                     /locus_tag="PKJ16_12310"
     CDS             complement(8522..>9060)
                     /locus_tag="PKJ16_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HNV47816.1"
                     /translation="GCGMINLWRKFMDSVPGKAEVTDLGGFFVPAFMNVAQAKVSMSA
                     KEARGLGYLGPKDKIVMNKDFLIGQAKKEVLRMVEDGYAPPAKKPIKVLGEEAMGMVL
                     ANLLNMRSGGYITPHMEEIAKRIAFCMSGGNVRSGSLVSEDTLMTLEREAFVDLWKTE
                     NTQKMAEHIMTTGKPLMI"
BASE COUNT         1997 a         2365 c         2643 g         2055 t
ORIGIN      
        1 tcggtgatcc ttttcgacga gatcgaaaag gcgcatcacg acatcttcaa cgtcctcctg
       61 caggtgctcg aggaggggga gctgaccgac agccagggcc agacggtgag ttttcgcgac
      121 accatcatca tcatgacctc gaatgtgggc aatgagcagt atcaccgggg cggccgcatg
      181 ggctttatca gcgaggggca ggccgtcgat gccgcacggg agggggtcaa cgacgagctg
      241 aaggaggtgt tccgcccgga gtttctcaac cggatcgacg aggtggtgca ctttcacaaa
      301 ctcgacaaaa agcacatgcg gcgcatcgtc gaaatcatgc tggatgacgt tcgcgagcga
      361 ctgcgcgata tgggcgtcga tatcgttttc tcctcgagcg caaagcggct gctggccgaa
      421 agggggttcg acgataagta cggcgcgcgc tttctccgac ggaccgttca gaacgagatt
      481 gaagacgcgc tcgcccttga cctgctcaag ggctccggcc gggagtgcgc gctaattacg
      541 gtgaccgtcc gcaagggacg ctttcacttt gccgcaagcg ccccgcagcc gtccgcgacg
      601 gccgatgccg aaacggcatg cgaaaaggca aatatcccgt aaccggaaag cgacggcggg
      661 aacggcgtcc gaagcgatcg gatcggcggg cgctctcgct catcaaccgc cgcttgaaaa
      721 aactcaccgc gcgcttcttc cttccgcgtt ttctccgcga gatgaagcgg ctcaagatcg
      781 gggggctcac cggcggcaac cgcctcaagc tcctggccga cggcgatagc tgctttcgcg
      841 agattatcgc cactatccgc aaagcgaaaa aaagcattaa tctcgagacc tacatcttca
      901 acagcgatgc gctcggctgg aagatcgcgg aactgctgat ggagaaatca cgcgccggcg
      961 tcgaggtgaa ttgcatctac gacgccattg gttgcatcgg gacctcgccg cggctctttt
     1021 ccgccctccg cgaggcgggc gtcgaggtgc ttgaatataa tccctttttt ccgtggcgcc
     1081 gctattggaa cctcagctcg cgcgaccacc gcaagatcat tgtcgtcgac ggcgagatcg
     1141 ccttcatcgg cggcatcaat atcggggacg actatgccgg caggcatttc gacggcggga
     1201 actggcgcga cacccatctc aaggtgcagg ggccggccgt gcgcgacatc cagttctttt
     1261 tcctggagaa ctggtatcgt cagggcggcg ctatcatcga taacggacgg cattttcccc
     1321 acatcaagga agcggggaaa aaactgctta tggtcatcag cgacaaggcg cggcggaagg
     1381 tgcgcccgat acgcgaatcc tatatctcgg ccatcgaaaa cgcgcatcac agcatttaca
     1441 tcacgaacgc gtatttcatt cccgacgggc gcatttaccg ggcgcttatc agggccgcgc
     1501 ggcgcggggt cgatgtccgc atcatgttgc cggagaaaag cgatgtcccc atcgtacagt
     1561 tcgcgtcccg gtacctgtac aagcgatatc tgaaatccgg cgttcgggtg tacgagtaca
     1621 cgcggtcggt tcttcacgcg aagacggcgg tcatcgacgg gatctggtcc acggtcggct
     1681 cgtcgaatat cgaccgcagg agtttccgca agaacctcga gatcaacgcg attattctcg
     1741 atcaggagtt cggggagatc atggagcggc agttcgacga agatttacga cactgtgtcg
     1801 agaagcgtct cgatgaatgg cagcgccgct cggcgatgct cttcgctctc gagtggatca
     1861 tgtatcggtt tcggaactac ctgtaggcgc ggttagatga agatgaggaa gcggccgcaa
     1921 tgggggcaga gcgaaatctt gtccgcgcgc ttcatgcggt ttgcgtcggc ggcggggatc
     1981 accatatggc acccggagca gctgttgttg tcgacgatcg aaaacggatt ctggcatttt
     2041 tgccgcatgc gctcgtaata ggtgagcgac cggttgtcga tgagcttttg taattcggcg
     2101 atctttttgg cggacgcctc gtttttcttc tttccgtcgg tgatctgata caggcggtcg
     2161 agccggatga tgagatcgag gttcgacgag ggcacgccgg acgggattgc gctcaccgcc
     2221 ttcttgatcc gatccttgct ggaaacgacc tcgagatatt tccttccttc ggtgttgagt
     2281 gcggaataga ccttagccgc cgtcgtcgcg gaatcgagca gcttggtctt tgatttttcc
     2341 gaaaaaatgg acgccatcat tcccaccaga tacaggtact tcgtgctcat atctgtcggg
     2401 aaaaggctga gcaggaggta cttgaccggc tgttggtcga aggcattgta ctcgatgccg
     2461 gcccggcaaa tcccgaacat gacgatgaga tttgaaacgg tgtccgtgcg gacatgcggt
     2521 atcgcaaaac ccttgccgat gcccgtgttt tcaagggatt cgcggtggat gacctgtgcg
     2581 taatatcggt cgacgttttc gattatctcg agcttgtgca gcttctgcag catctccttg
     2641 atgacgagct gcttttcgtt actcttcagg aatatgatgt gcttgctggg gaaaaagtcc
     2701 tcgagattca tgtcgcccgc attgtgggag aatggaatta aaaattcggg gcgagaggat
     2761 ttgaacctcc ggccccttga cccccagtca agtgcgctaa ccggactgcg ccacgccccg
     2821 aatatttaat gcatcattgt tttttccggg tctggtgtaa agaaaaaatt ttctcatttg
     2881 gccgtttttc gtgaaatacg ggagagccac tcatcgattg catacagggc gaacgtgtcg
     2941 ttttctcggg tgatcaccca gcgcgccccg cgctcagtaa gctctcgaat cgtttttggg
     3001 tcggcaagct cgttttcgaa aagggtccat ccatgacggc ggaccagatg gaggtagagc
     3061 ggaggcgttc cggtgttgat gataacgacg cgatcgaacg gcgccgtttt ttcagcgacg
     3121 agcggttgga gccgaacaag actcttgaca tcgtcatcga cgcgataggt gtagccgatg
     3181 gcgatgaaaa aaatcgctcc gagaacgacc agcagtgcat aataatagtt tcgcatgagc
     3241 tcgtgcgcat tgcggagccc atatgcgatc agtatggccg cagagggaat aaaggcgaaa
     3301 aagtagtaat aatgggggga gaaatggcgt ccgatgatga cgaaaagcgt gagcgcggtc
     3361 gccagcggta tgatccacac aacggtgtgc gcaagataac cggggagcgg ccgatgggca
     3421 gtctcgtctt gatcgggcga attcgcccgg agccccctgc gaaaaccgac gattcccgca
     3481 atggcgacgg gaatcagcgc atatccggta tagtattgga aaaccgtttt gaagaattct
     3541 atccacaacc cgactgacgc catctcggcg agcgtttgcg aaagggaata cccgaagaag
     3601 aaaatgttga gcccatagct ccgtacgagg tgcggcgccc agaggtaata ccaggcgacg
     3661 aatggaataa tgatggagac accggtgagc agaacaccga aacgaaagcg acgctggaag
     3721 gcaagcgagt agatgaatat caccagcagg gggaggcaga aaaaaatcaa aaacggtcga
     3781 atgagcgccg cacaggcgag gcacatggcg gcgatcacaa gatggcgcgc ctttggatcg
     3841 tcgtggtatt gcagcgtggc gtacatgccg ccgacaccga acagtaagcc ggttatggac
     3901 ggcatgtatt tcccggcgaa taggaaaaat gcgggaacaa agaaaagcgc ggcgtaggaa
     3961 tagcgccgct cgatcccgaa aaatcgacac agcaaagaac cgagaagaag aaaaacgccg
     4021 agcgccgaga caagcgagag cagctttccc cagccatcgt ggagcgagcc cgtgatgcga
     4081 tggataatcg atgccacata ctggtacagg ggaaactcca ttccggtgat gccggaagtc
     4141 tcgccgcggc ggtcgacgcg gggatagagt atgttcggcg attcgacggc gaaatttcga
     4201 gccaccgcag ccgtatccgc ctgccggagg cggtgcgatc cggttatcgg ggtgagaaga
     4261 tacgggacat gggtaaggac aaaaataacg agaaatgcat acgcgattat cctgtctcgt
     4321 cgatcgtcca tgtgatgttc cccgtttatt gaaaaatgtc gcctgtttct ttataccaga
     4381 gaacgaaatc gagataatca agcggaatat tgatcgattg ggaaaaatct cgcatcgcat
     4441 tttcgtatgt caggtaccga gccgccgaca ggttcgacgg agggccgtca atgacgccga
     4501 acgccgtgag gtttttcagg atgtgacgat cgaggatcgc gagcgaaccg ccatggccga
     4561 tgttccggag aaaatggctg gcctccttgt agccatatcc cgtcaccgcg gatgcgagcc
     4621 aggaacgcat gtcaaaggca acggcatgtt gatccaaaat gcttcgcagt gatggtcgat
     4681 ctagtccata gagggtctcc cgcgcccgca cgatgtattc ggccttcttg ttgcggaaac
     4741 ggacgatcgg aatgcgcgcc gcgatatccg ccggacggcc atggaagagc aggtcgtcct
     4801 cgaggagaat ctgcaccgcg cgccagcact gacgggcctt cgattgcggg gtgaggaggc
     4861 aaaacaccag ttggatgaat agctcccgat cggtggcgaa ttcccaacgg cgggagaaga
     4921 gctcgagctg ccgagatacc tcgacgcgga ttgaatcata tattgaacgc aactcgcttc
     4981 cgcgatcatt ccgatgtttc attggattac tgtatcttca catgaggtcg ttcacggtgc
     5041 tgcaattcgt gtaacggagg aagtctagcg caactggacg attggggcga gattattcca
     5101 acagatttga acagatatcc ttaaggctga gaatccgctt caggagcgaa tccaataccg
     5161 ggtcgtcata gtgttttgcg aacagggtat aggtcttcgt ggccattcga tcgatgatgc
     5221 ggccgagtga cgcaagcgtc tcgcggtcgt acgaggtgag cgagagcgcg attgcaatgg
     5281 tctcctcaag cgcgtcacgg tcattctcga atgacctcat atcggaaatg tcctcgatca
     5341 agtcgcggca catgaagacg agcgtctgtt gcatcgaaaa gcgggcgggt tcatccatgg
     5401 gcggcatggg cggttacccg gttatggcta tttcttcgac aagggccgaa aagagtgtta
     5461 tgatgtcggt agtcgccttg cgcagctttc cgcagatctg gcgtgcgatc tcgcgggtta
     5521 tcgcaagccc gatttccgga ttcgcattcc cgaatgcgat aaaatccttt ctgcttattg
     5581 ccaggcagat gcagtcggtt tttgcgataa cggtcgccga gcgcatgtca ttgtcgatga
     5641 gcgccagttc tcccacgtag acgccgccga gcaccgcatc aagggtggtc accgtgtact
     5701 tctcattttg catcgttttt tttacgatct cgattttacc ggagaggacg atgtagagtt
     5761 catcgccgta ctcgccttcg cggataatga gcttcccgcg cgcaaagcgg cgttttcggc
     5821 agatcatcga tatctttttt atgatatcgt catcgtccgc gaacatgcgc aggaatgaca
     5881 ccttcttgag cgtgtgtgcg atctcgccgt tgccgcttgt gacagttcga agtagcatta
     5941 tgatgccgcc tgtgccggat ggatgtcgtg gccctcgatg atgatggccc gcgagtgttt
     6001 gccgatgata tactctggat gtgggttgag gatcggattg ttcgggatga gattcttcac
     6061 gagtttcagg ttgtccacca gggtcgagat atccggcgtc ttttgggcct cgcgcagagc
     6121 ctcggttttt ctatcgtaga agttgccggt gttttcaaga atgccaatga gtatcgagtt
     6181 gtctcgtttg cgcacatgct gaaacaactc ctcgtacgtt tttccaacga attctccggg
     6241 tatgtcgatg gtgttgatgg aaaccgaggc atcgacgttg agcagctcgc caacgatatg
     6301 ggagatgccg ttcccgcatg atgcgttcgc gatgaccgac cggttgtagt cggaggtcag
     6361 gacgatctcg tcacagttgg aaaagcggag atagcgctcg aactttgagt cgagaagctc
     6421 ggcgcaggtg taaatcgact tcgagataga ctttatggtt atgatcgaca tcacggtacg
     6481 cgaatcgacc tcttgtacgt ttccggtgac catgcgatct gccaggacaa ggattttact
     6541 cgcatgacgc agggcggcgc gattgagtat tttctcgtcg atgtgatcgc cgtgaatgta
     6601 atggatcgcc gtgagatcgg cgtccgcccg caggctctcg acctgctccg ggtcggcggt
     6661 gttaatgaga acgatctcgg agggaagaaa attcttgttc ttcgccagga tgtcgcgaag
     6721 gaacttcccc atgtcgcgct tccaaccgca gataatgaag tggttcttta atcgtaatcc
     6781 tttcaagcct ctgccttcct tgagctgttt ttcaaccaat atcgatgaaa tattaccggt
     6841 tataaatcct acgagtagaa tgccgaacag catcgttaca atgccaaaga ggcggccggc
     6901 atgggttttt gggtacatgt cgccatagcc caccgttgtc gacgttacga ttgcccacca
     6961 gatggagtcc tcgattgtcc ggatattgct cgatttgtcg ttgtcctcga cccggtttcc
     7021 ctgttcgatt ttatacgcta cattgtcgcg ctccatatag tatacgccaa aggaacacag
     7081 aatcattacg atgatgcccg cggtgaggaa cttgaaaatg acggtattga atatccgtat
     7141 aattatgtat ttcagttcgc gtaattgttt catatggtta tgatttcgat gatatcacca
     7201 tgtatctata ttaaatgatc ggaatggtac tcagggcggg gaaacaaaaa agccgcccga
     7261 aggcggcttt tttgggaaat cctgtactac ggtactagtc gcagagctcg aagagggccg
     7321 ctgcgcccat gccgccgccg atgcacatcg actcgacgcc gtacttcttg ccgaggcgct
     7381 gcatgttggc aacgatggtt gcagccagct tggagccggt gcatccgagc gggtgaccga
     7441 gcgcgatcgc gccgccactc acgttgatgc agcgcgaagc gctgtcggcc gcccaattct
     7501 tcttgtcgtc aataccgatc tgctgggcgc tgtagattgc ctgagacgcg aaggcctcgt
     7561 tgagctccca gattccgata tcggacgctt tgaggccggc catcttgagg agtttcggaa
     7621 ccgcatatgc gggtccgacg cccatctcgt cggccttgca tccggcgacg gtgtacatct
     7681 tgaacttcac gagaggcttt acgccgtatt tcttgacggc ttcttcgctc atgaggacag
     7741 aagctgccgc accatcggtg gtctgcgagg agttcccggc ggtaacggaa cccgttgaag
     7801 agaaagcggg tttgagcttc ttgaggccgt cgatggtggt ttccttgcgg acgccatcgt
     7861 cgatcgactg cataaaggtc tctttttccc acttgccatc cttaaggacg aacttctcag
     7921 cgggggtggg gactatttca gtgaaaaagc ccttttcctg ggcttcggcc gccttcatgt
     7981 ttgaatgata ggcgaactcg tcctgcatct cgcgggtgat cttgtagcgg ttggctacgt
     8041 tttcggccgt gatccccatc gaaatgtaca cgtcggggtt cttccttgac cagtccggat
     8101 gcggacgggg catgttgcca cccatcggga ctaccgacat cgactcgagg ccgccagcca
     8161 tggtcacttc cgaccatccc atcgcgatcc gcatggcgga gatggcgatc gactcaagac
     8221 cggaggcgca gaaccggttg acgaccgctc cagagacgga atccgggaag ccggccatct
     8281 gtgcggcaat gcgaccgatg ttgaggccct gttcggcttc cgggaaggcg cagccgatca
     8341 taacgtcgtc gatagccgaa acgtcgaatt tgattttttt gactgccgca tcgagaatgt
     8401 gcgccagcaa cgattccggg cgggtttgcg cgaaggcgcc cttcgcccgc cggcaaccgg
     8461 gagttctaac agcacttaca atatatacat ctctcatata atcctcctga atgtaatttc
     8521 cctatatcat taagggtttc ccagtcgtca tgatgtgctc ggccatcttc tgcgtgttct
     8581 ccgttttcca gagatcgacg aaggcttcgc gctcaagcgt catgagagta tcttcggata
     8641 cgagggagcc gctgcgaacg tttccgcccg acatacagaa ggcgatgcgc ttggcgatct
     8701 cttccatgtg cggggtgatg tagccgccgc ttctcatatt gaggaggttg gccagaacca
     8761 tacccatcgc ctcttcaccg agcaccttga tcggcttctt tgcggggggt gcgtatccgt
     8821 cttcaaccat tctcaggact tctttcttgg cctggccaat gaggaaatcc ttgttcatga
     8881 cgatcttgtc tttgggaccg aggtagccga ggccgcgcgc ttctttcgct gacatggaca
     8941 ccttcgcctg ggcgacgttc atgaaggccg ggacaaagaa accaccgagg tcggtgactt
     9001 ccgcctttcc gggcacggaa tccatgaact tcctccatag gttgatcata ccgcaaccgc
//
LOCUS       DAORHF010000150         9059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7100_length_9059_cov_4.903709, whole genome shotgun sequence.
ACCESSION   DAORHF010000150 DAORHF010000000
VERSION     DAORHF010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_9059_cov_4.903709"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1258)
                     /locus_tag="PKJ16_12315"
     CDS             complement(149..1258)
                     /locus_tag="PKJ16_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47817.1"
                     /translation="MTTLRKTIGTLFEWEITMIPAEVTRRESRLAGVYLSLFGVVFCG
                     LAIGSVVLSLSTGRFDPIVLAVAIPFAGIGVAVLLIGVNKLRYRLFMRIDSETVFVRL
                     QSLFRNDEWRELLSGYRGVMLRIVESGEQTVRVIELHHPDPMRRVILAEHLVGIVSER
                     DTRARWESYARIINLPALEDAGAGVVERAPGDLDRPYRDIHAAEDAPAVSSARPRYIT
                     IEEEGDSQTVVIGFKKMRSWRTLIGALIPAALMSIGFLLPVRLTVEFVAIGLFGALIG
                     VVLIAAIAWDIVASPMIHADSFGIRVAYRTLCGDTTGKRIPYGEIEEVIVGKEPNNPY
                     STVLIRSDARTMSVGHAMPMDDLVWLRDYLRGRIR"
     gene            1410..2231
                     /locus_tag="PKJ16_12320"
     CDS             1410..2231
                     /locus_tag="PKJ16_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HNV47818.1"
                     /translation="MHAIKGLVKSAVPRGAYAALYSLFIRLRGLSSILYRGDAVECPF
                     CGGRFTRFLAWGLKLPVIDEKRIIGAGFRENALCPRCRSFDRERLLYLYLRDQTRIFT
                     ASDKKIRLLHIAPEWHIQRVLSKRPGIDYTSADLSSPTAMVKLDVTAIQFADQSFDAV
                     ICNHVLEHIPDDRKAMSEIYRILKPGGFAILQVPIALALEKTVEDPSIDDPKKREELF
                     GQDDHVRVYGKDYAERLRDVGFVVEAVNYAREIGDEAAKRYGLLRDETVFVGVRS"
     gene            complement(2241..3287)
                     /gene="argC"
                     /locus_tag="PKJ16_12325"
     CDS             complement(2241..3287)
                     /gene="argC"
                     /locus_tag="PKJ16_12325"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HNV47819.1"
                     /translation="MKKALIVGATGFGGLGLIEILLRHPGIEIKQLVARKDVGVPVSA
                     VFPHLRGFCDMVVDAPERIDFANVDIAFFSTPDRAGMTMINDFRDKGIPVIDFSGDFR
                     FRSTDEYGVYARNKGMADGHLAADVIGEAVYGLPEIYADEIRSAKIVGNPGCFAICMI
                     LGLLPAVAGGIVDASTIVCDGKTGVSGAGKNPGEANFYPQRYENVNTYREGRHQHLVE
                     VENILNREGSSPVKVFFVPQIVPLNRGIMITAYAPAKAGINTAKAASLYRDYYMGKPF
                     VVITDTSPATTDVRGSNRCEIRPMVDERTGTLFVTAVIDNLVKGQSGNAVQNANLILG
                     LDETLGLNVPAFYP"
     gene            3542..3862
                     /locus_tag="PKJ16_12330"
     CDS             3542..3862
                     /locus_tag="PKJ16_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47820.1"
                     /translation="MNDYYKIAEAVARVLGAEDKPAITPDGMKNTVFTIAFRTKAGAD
                     MTARLTGDRFTWRITMPRNMFAAMPFERFLSKLEYELEQVFMRNIKIAAIDEVSGYQI
                     TLTL"
     gene            complement(3884..4594)
                     /locus_tag="PKJ16_12335"
     CDS             complement(3884..4594)
                     /locus_tag="PKJ16_12335"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HNV47821.1"
                     /translation="MKAISVALPLVTAFLCGFCGAGGRSDVEQLREQMVKRQIEARGV
                     RDARVLSAMRAVERHRFVPTASLHQAYGDHPLPIGGGQTISQPYIVALMSELCRLRGD
                     ERVLEIGTGSGYQAAVLSLLTKEVYSIELVESLGKAAIKRLGELGYANVRVRIGDGYK
                     GWPEMAPFDVIIITAAPPEVPPMLFEQLKEGGIMVVPVGELWQELITVEKENGRMIRK
                     TITGVRFVPMVRGSDGGR"
     gene            4819..6567
                     /gene="ptsP"
                     /locus_tag="PKJ16_12340"
     CDS             4819..6567
                     /gene="ptsP"
                     /locus_tag="PKJ16_12340"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HNV47822.1"
                     /translation="MPQRIKGTVASPGIRIGKAYVYQTSKVYIPKYDITVDQIDAEII
                     RFEDAIRKTKEEIKSIQGQIATSLSKDMADIFSSHIMMLEDPQIYEKTKELVRSELRN
                     VEWVLNDTALDLMKGFSAIEDEYLRERILDISDIHKRLISNLQKQKVDSVSLANLKEE
                     VIVFASDLTPSDTAMMNKKYVLAFVTDKGGRTSHTAIMARALELPAIVGTMNATSMVR
                     DGDTVICDATHGEVLINPTPELIEEYTRYQEELRAMEIELAKLTSLPSLTVDDEEVFL
                     YGNIELPDEMAVIKEHGAQGIGLFRSEFLFLGRSLPDENTQFCEYRKVVEFFNPLPVT
                     IRTLDVGGDKIFSYTNEYRERNPFLGCRAIRFSLENVDLLRTQIRAVLRASHYGTVKL
                     MFPMVSTVEEIVRAREILDETRDELDRAGIPFDHEIAVGVMIEVPSAVIAGDLLAKHS
                     DFFSVGTNDLIQYTLAVDRISEKVAYLYDPLNISVLRFLKQIVVIARDADKPLSICGE
                     IAGEPIYTMLLLGLGFRHFSMSSASMNQIKRIIRAVRIEDCNRLADELLSMEKAADIE
                     RRLMEVTAREFPWLTL"
     gene            6574..7329
                     /locus_tag="PKJ16_12345"
     CDS             6574..7329
                     /locus_tag="PKJ16_12345"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV47823.1"
                     /translation="MKLYHELAEYYFAIENKHRDIDDDIALIRSLCAGMRAPAILDLG
                     CGTGEHLHELAKAGFACTGIDNSEEMLRIARLRFPDDAEFIHDSMLSFDRFEEFDIAV
                     SLFGSLDYCISDDDVDKVFWNTWRSLKPGGTGLFEVWNAVPIKKIREKDTGHVSTTIY
                     QHRVVIDRERGFKLLPYPGKTVVEVSYVYSIQKDGAEENVTDRHVMRAFDKDEITAFI
                     ENNGFRVQQYFASSQRELYKETSNKILVHFVKS"
     gene            complement(7701..>9059)
                     /gene="lon"
                     /locus_tag="PKJ16_12350"
     CDS             complement(7701..>9059)
                     /gene="lon"
                     /locus_tag="PKJ16_12350"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV47824.1"
                     /translation="GRSVARALGKNFVRMSLGGVRDEAEIRGHRRTYVGALPGRIIQM
                     MKKAGSVNPVFLLDEIDKMSMDFRGDPSSALLEVLDPEQNFAFVDHYMEVPYDLSKVM
                     FITTANVQHRIPLPLQDRMEIIELSSYTEPEKLNIALQFLLPKQIEENGLKPEHIIYS
                     DEMMLSTIRHYTKEAGVRSLERVIAKVCRKVAKEVVRTKTGHVSTITEELLGQFLGPK
                     KFKYGTKELANETGLANGLAWTEVGGDILQIEASLIKGKGSLILTGKLGEVMQESGRA
                     AFTYVKANQQFFNIDEDILKKHDIHLHVPEGAIPKDGPSAGITMAVVIASLLSGIPVK
                     CDLAMTGEITLRGKVLPIGGVKEKVLAADRADITHIIIPKENGKDLEKIPDYVKKRVS
                     FHVVSNMEEVLFLSLESPEKFFKDQEKAKRLYDQWKGELGDPAGGVDGEKPWDAEKPV
                     HM"
BASE COUNT         1793 a         2935 c         2648 g         1683 t
ORIGIN      
        1 gtggtgaacg aaaagtacgc caaagaaatc gaggcgatgt acgccggcga caccggagac
       61 tagaaacgga cagcaccgca ctcatgcaaa acgccgcgca tatgcgcggc gttttgcata
      121 ttcccgccgg catacgccgg aagcggcctt atcgaatgcg cccacggagg taatcccgga
      181 gccatacgag atcatccatt ggcatcgcgt ggcccacgga catggttcta gcgtcgctgc
      241 ggatcagcac cgtcgagtac gggttgttcg gctccttccc gacgatcacc tcctcgatct
      301 cgccgtaggg tatgcgcttg ccggttgtat cgccgcatag agtgcgatac gcgacacgaa
      361 tcccgaacga atccgcgtgg atcatgggcg atgcgacaat gtcccaggcg atcgcggcga
      421 tgaggaccac tccgataagc gcgccaaaga gcccgatggc gacgaactcc accgtaagcc
      481 gcacgggaag gagaaacccg atcgacatga gggccgccgg gatgagcgcg ccgatgagcg
      541 ttcgccacga gcgcatcttc ttgaacccga taacgacggt ctgcgagtcg ccctcctcct
      601 cgattgtgat atagcgcggt cgcgcgctcg atacggcagg agcatcttct gccgcgtgaa
      661 tgtcccgata cggccggtcg agatcgcccg gcgcgcgttc caccaccccg gcgcccgcgt
      721 cctcgagcgc cggcaggttg atgatgcgcg catacgactc ccagcgcgcg cgcgtgtccc
      781 gctcgcttac gatgcccacc aggtgctcgg cgaggataac gcggcgcatc gggtccggat
      841 gatggagctc gatgacgcgc accgtttgct ccccgctctc gacgattcga agcatgacgc
      901 cgcgatagcc cgaaaggagc tcgcgccatt cgtcgttcct gaacagcgat tgcaggcgca
      961 cgaagaccgt ctcgctgtcg atgcgcatga acagccgata gcgcagcttg ttgacgccga
     1021 taagcagcac cgcgacgccg atacccgcga agggaatcgc caccgccagc acgatgggat
     1081 caaaccgccc ggtggagagc gagagcacga ccgacccgat cgccaatccg caaaatacga
     1141 cgccgaagag cgagaggtag acgccggcca gacgcgattc gcgccgcgtg acctccgccg
     1201 ggatcatggt gatctcccat tcgaacagcg tgccgatggt ttttctcagg gttgtcatgt
     1261 gaacgctcct ttccgcattg atacgcgaca ccgcgggcgg aatcggcacc gcccgggccg
     1321 tacggcgccg gcgcagtgaa atttggattg caaatatcgg gcgcatcggc aatgacgatt
     1381 ttcaatcatc aatacaagga aaccggacca tgcacgcaat caagggtctc gtcaaatcgg
     1441 ccgtcccgcg gggcgcctat gccgcgctat attcgctttt tatcaggctt cgcggattgt
     1501 cgtcaattct ttatcggggc gacgccgtcg agtgcccctt ctgcggcggg cgcttcacgc
     1561 gcttcctcgc ctgggggctc aagctcccgg tgatcgatga gaagcggata atcggcgcgg
     1621 ggttccgcga gaacgcactc tgcccgcgct gccggtcgtt cgaccgcgaa cggctgctgt
     1681 atctctatct gcgggaccag acgcggatct tcacggcgag cgataagaag atccggctgc
     1741 tgcacatcgc tccggaatgg cacatccagc gcgtcctctc gaagcggccg gggatcgact
     1801 acacgagcgc cgatctctcg tcgcctacgg cgatggtgaa gctcgacgtg accgccatcc
     1861 agttcgccga ccagagcttc gacgcggtca tctgcaacca cgtcctcgag cacatccccg
     1921 acgaccggaa ggccatgagc gagatctacc gcatcctcaa gcccggcggc ttcgcgatcc
     1981 tgcaggtccc catcgccctt gcgctcgaga agaccgtcga ggacccgtcc atcgacgacc
     2041 cgaagaagcg cgaggagctc ttcggccagg acgaccacgt gcgtgtgtac gggaaggatt
     2101 acgccgagcg cctgcgcgac gtcgggttcg tcgtggaggc ggtcaactat gcgcgggaga
     2161 tcggcgacga agcggcgaag cggtatgggc tactaaggga cgagaccgtg tttgtggggg
     2221 tgcggagctg aaccgccccc tcacggataa aacgccggca cattaagccc caacgtctcg
     2281 tctaacccca gtatgaggtt cgcgttctgc acggcattgc ccgactgccc cttcacgaga
     2341 ttgtcgatca ccgccgtcac gaagagcgtg cccgtgcgct cgtccaccat cgggcgtatc
     2401 tcgcagcggt tggagccgcg cacgtcggtc gttgcgggcg acgtatcggt gatcaccaca
     2461 aacggtttgc ccatgtaata atcgcgatac agcgacgccg ccttcgcggt gttgatcccg
     2521 gccttcgccg gcgcgtaggc ggtgatcatg ataccgcgat tgagcgggac gatctgcggg
     2581 acgaagaaca ccttgaccgg cgaggagccc tcgcgattga gaatattctc cacctccact
     2641 aaatgctgat gcctcccctc gcgataggtg ttcacgttct cgtagcgctg cggatagaag
     2701 ttcgcctcgc cgggattctt ccccgcgccg gacacgccgg tcttgccgtc gcacacgatg
     2761 gtggacgcgt ccacgatccc accggccacc gccggcagca gccccaggat catgcagatg
     2821 gcgaaacagc cggggttgcc cacgatcttc gcgctcctga tctcgtcggc gtatatctcc
     2881 ggcagtccgt agacggcctc gccgatcacg tcagcggcga ggtgcccatc ggccattccc
     2941 ttattgcgcg cgtacacgcc atactcgtcg gtggagcgaa agcggaagtc gccgctgaag
     3001 tcgatgaccg ggatcccctt atcgcggaaa tcgtttatca tggtcatccc ggcgcgatcg
     3061 ggcgtggaga agaaggcgat atcgacattc gcgaagtcga tccgttcggg cgcgtccacg
     3121 accatgtcgc aaaagccgcg caggtgcggg aacaccgcgc tcacgggaac gcccacgtcc
     3181 ttgcgcgcga cgagttgctt gatttcgatg ccgggatggc gcagcaggat ctcgataagt
     3241 cccagccccc cgaatccggt cgcgccgacg atcagagcct tcttcatgtc attccccctg
     3301 tcgattgata tccacgtcct tcttcacgcg gcaacggccg atcgtcaatc attttcaaag
     3361 gtgtcgattg gggacgcgag acggcgtccc gatggattcg agcaagcgcg tggcgtcgga
     3421 tataactttc ttgacagaag accgtcacac gattgtgctt atcatgcacg gcgcacacat
     3481 ttcattttta ctgggccagg accacgggtc gcgcagatca ttgtgacggg agacgggaac
     3541 catgaacgac tactataaaa tcgccgaagc cgttgcgcgc gtactcggcg ccgaggacaa
     3601 gcctgccatc acccccgacg gaatgaagaa caccgtgttc accatcgcgt tccgcacaaa
     3661 ggccggagcc gacatgaccg cgcggctcac cggggaccgc ttcacctggc gcatcaccat
     3721 gccccggaac atgttcgcgg cgatgccctt cgagcgcttc ctctcgaagc tggagtacga
     3781 gctggagcag gtcttcatgc gcaacatcaa gatcgccgcg atcgacgagg tctcggggta
     3841 tcagataacg ctcaccctgt aaacgccgcc gtgggcggtc ccgtcaccgc ccgccgtccg
     3901 acccgcgcac catcggcacg aaacgaacgc cggtgatcgt ttttcgtatc atccgtccgt
     3961 tttccttctc cacggtgatg agctcctgcc acagctcgcc caccggaacc accatgatcc
     4021 cgccctcctt gagctgctcg aagagcatgg gcggcacctc cggcggcgcg gcggtgatga
     4081 tgatgacgtc gaacggggcc atctccggcc agcccttgta cccgtccccg atccgcaccc
     4141 gcacgtttgc gtaaccgagt tcgcccaagc gcttgatggc ggccttcccc agcgattcga
     4201 ccagctcgat ggagtagacc tctttcgtca ggagcgagag gaccgccgcc tgataaccgg
     4261 agcccgtgcc gatctcgagg acgcgctcgt ccccgcgaag gcggcagagt tcgctcatga
     4321 gcgcgacgat gtacggctgc gagatggtct gcccgccgcc gatgggaagc gggtggtcgc
     4381 cgtatgcctg gtgcaggctc gccgtgggaa cgaaccgatg gcgctcgacc gcgcgcatcg
     4441 ccgagagcac gcgcgcatcg cgcaccccgc gcgcctcgat ctggcgcttc accatctgct
     4501 cgcgcaattg ttccacgtcg ctcctccctc cggccccgca gaaaccgcac agaaacgccg
     4561 tcacgagcgg cagtgcaaca gaaatcgctt tcatggcagc ctcaacaatc gcgggcagtg
     4621 cgcaccgcgc ccacattcag attatagcga tgctaaaaaa taatcaagaa aaaatcaccg
     4681 agtaatttta ccccgcgagc gaaaatcctc ttgactttct ttttcgttat ggcgttgtga
     4741 tgaaaatcga gaaccgggac cgtccgcgcg acgcccggcg tgcgccaatc cgcatgacag
     4801 cataatcaga gaccccgcat gccacaacga atcaagggaa ccgtcgcctc tccgggcatc
     4861 agaatcggca aggcctatgt ctaccagaca tccaaggtat acattcccaa gtatgacatc
     4921 accgtcgatc agatcgacgc ggagatcatt cgcttcgagg atgccatcag gaaaaccaag
     4981 gaggagatca agtccatcca gggccagatc gccacgagcc tgtcgaagga catggcggac
     5041 attttctcca gccacatcat gatgctcgag gacccgcaga tatacgaaaa gaccaaggag
     5101 ctcgtccgca gcgaactgcg gaacgtcgag tgggtgctca acgacaccgc gctcgacctc
     5161 atgaagggat tcagcgccat cgaggacgaa tacctgcgcg agcgcattct cgacatctcc
     5221 gatatccaca agcggctcat cagcaatctg cagaagcaga aggtcgacag cgtatcgctg
     5281 gccaatctca aggaggaggt gatcgtcttc gcctccgatc tcacgccgtc cgacacggcg
     5341 atgatgaaca agaagtacgt gctcgccttc gtgaccgaca agggaggacg cacgtcgcac
     5401 accgccatca tggcgcgcgc gctggagctg cccgcgatcg tgggcaccat gaacgccacc
     5461 tcgatggtgc gcgacggcga caccgtcatc tgcgacgcca cgcacggcga ggtcctcatc
     5521 aaccccacgc cggaactcat tgaggagtac acccgctacc aggaagagct tcgcgcgatg
     5581 gagatcgagc tggccaagct cacctcgctg ccgtcgctca ccgtcgacga cgaggaggtg
     5641 ttcttgtacg gcaacatcga gctccccgac gagatggccg tgatcaagga gcacggcgcg
     5701 caggggatcg gcctgttccg ctcggagttt ctgttcctgg ggcgctccct gcccgacgag
     5761 aacacgcagt tctgcgaata tcgcaaggtg gtcgagttct tcaatccgct gcccgtcacc
     5821 attcgcacgc tcgatgtggg cggcgacaag atcttcagct acaccaacga gtaccgggaa
     5881 cgcaatccgt tcctggggtg ccgggccatc cgcttcagcc tggaaaacgt ggacctgctg
     5941 cgcacgcaga tccgcgccgt gctgcgcgcg agccactacg gcaccgtgaa gctcatgttt
     6001 ccgatggtct ctacggtcga ggagatcgtc cgcgcccgcg agatcctcga cgagacgcgc
     6061 gacgaactcg atcgcgcggg gattcccttc gaccacgaga tcgccgtggg cgtcatgatc
     6121 gaggttccct cggcggtgat cgccggggac ctgctcgcca agcattcgga cttcttctcg
     6181 gttggcacca acgatctcat ccagtacacc cttgcggtgg accggatcag cgaaaaggtg
     6241 gcctatctgt acgatccact caacatatcg gtcctgcgct tcctgaagca gatcgttgtg
     6301 atcgcgcgcg acgccgacaa gccgctctcg atctgcggcg agatcgcggg cgagcccatc
     6361 tacacgatgc tgctcctggg cctgggcttc cgccattttt cgatgagctc ggcgtcgatg
     6421 aaccagatca agcgcatcat ccgcgccgtg cgcatcgagg actgcaatcg gctcgccgac
     6481 gagcttctct ccatggaaaa ggccgcggac atcgagcggc gcctcatgga ggtgaccgcg
     6541 cgcgaattcc cctggctcac cctgtagcgg cgcatgaagc tgtatcacga gctggccgag
     6601 tactatttcg cgatcgagaa caagcaccgc gacatcgacg acgatatcgc cctcatccgt
     6661 tcgctctgcg ccggcatgcg cgccccggcc atcctggacc tgggctgcgg gaccggcgag
     6721 cacctgcacg agctcgcgaa ggccggtttc gcctgcaccg gcatcgacaa cagcgaggag
     6781 atgctgcgca tcgcgcgcct gcgcttcccg gacgacgccg agttcatcca cgacagcatg
     6841 ctctccttcg accgcttcga ggagttcgac atcgcggttt cgctcttcgg ctccctcgac
     6901 tactgcatct ccgacgacga cgtggacaag gtcttctgga acacctggcg ctcgctcaag
     6961 cccggcggga cggggctctt cgaggtttgg aacgccgtgc ccatcaaaaa aatacgcgag
     7021 aaggacacgg gacacgtctc gacgacgatc taccaacacc gtgtggttat tgaccgcgag
     7081 cgcggcttca agctgctgcc ctatcccggg aaaacggtcg tcgaggtgag ctatgtctat
     7141 tcgatacaga aggacggcgc cgaggagaat gtcacggacc ggcatgtgat gcgcgccttc
     7201 gacaaggacg agatcaccgc cttcatcgag aacaacggct ttcgcgtgca gcaatacttc
     7261 gcgagctccc agcgcgagct ctacaaggaa acctcaaaca agatactggt gcatttcgtt
     7321 aagtcctagc ggtcccctgc gtcctccccc ggcgcctcga tgtccgcgag gtccttgtgg
     7381 cgacattatg ggcgttcaat aactcgagca actctttgaa gtctttctga atttccatga
     7441 agctgcctgc gcagggtttc aacggcctca agccgctctt ccggggatcg gctcatccac
     7501 caggaaaggt cgtcggacgg tttcagttcc gacggttttc tttttaacgc gcgtttaatc
     7561 atgccatcgt gaagttgatt attgcagaac cggcgccagg gaaagtgcgc gcacaattcc
     7621 cgtgatcacc ccaataagca aaaagccccg cacagagcag ggctttcgtt ttctgttaat
     7681 cgaacgatgc aaccggcacc tcacatgtgc accggcttct ccgcatccca cggcttctcg
     7741 ccgtccacgc cgccggcggg atcacccagc tcgcccttcc actggtcgta gaggcgcttc
     7801 gccttctcct gatccttgaa gaacttctcg gggctctcca gcgacaggaa gagcacctcc
     7861 tccatgttgc tcacgacgtg gaacgacacg cgcttcttta catagtccgg gatcttctcc
     7921 aggtccttgc cgttttcctt ggggatgatg atgtgcgtga tatcggcccg gtcggccgcg
     7981 agcaccttct ccttcacgcc gccgatgggc agcaccttgc cacgcagggt gatctcgccg
     8041 gtcatcgcga ggtcgcactt caccgggatc ccggagagga gcgacgcgat caccaccgcc
     8101 atggtgatgc cggccgacgg cccgtccttg ggaatggcac cctcgggcac gtgcaggtgg
     8161 atatcgtgct tcttgagtat gtcctcgtca atattgaaga actgctggtt cgccttcaca
     8221 tacgtgaacg ccgcacgtcc cgactcctgc atcacctcgc ccagcttgcc ggtgaggatg
     8281 aggctgccct tgccctttat gagcgaggcc tcgatctgga ggatgtcgcc gcccacctcg
     8341 gtccaggcaa ggccgttggc gagaccggtc tcgttggcga gctccttggt gccgtacttg
     8401 aattttttgg gacccaggaa ctggcccaag agctcctcgg tgatggtcga gacatgtccc
     8461 gtcttggtgc gcaccacctc cttggcgacc ttgcggcaca ccttggcgat cacacgctcc
     8521 aggctgcgca cgccggcctc tttcgtgtag tggcggatgg tcgagagcat catctcgtcc
     8581 gaatagatga tgtgctcggg cttgagcccg ttctcctcga tttgcttggg cagcaggaac
     8641 tgtaacgcga tattcagctt ctcgggctcg gtatagctcg agagctcgat gatctccatg
     8701 cggtcctgca gcggcagcgg aatccggtgc tgcacgttcg cggtggtgat gaacatcacc
     8761 ttcgagaggt catacggcac ctccatgtag tggtccacga aggcgaaatt ctgctcgggg
     8821 tccagcacct cgagcagcgc cgacgacggg tcgccgcgga agtccatcga catcttgtcg
     8881 atctcgtcca ggagaaagac cggattcacg gagccggcct tcttcatcat ctgtatgatc
     8941 cggccgggga gcgcgcccac gtaggtgcgg cgatgcccgc ggatctcggc ctcgtcgcgc
     9001 accccgccca gcgacatgcg cacgaagttc ttgcccagcg cccgcgcgac cgaccgacc
//
LOCUS       DAORHF010000151         9048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7117_length_9048_cov_4.866229, whole genome shotgun sequence.
ACCESSION   DAORHF010000151 DAORHF010000000
VERSION     DAORHF010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9048
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7117_length_9048_cov_4.866229"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..921)
                     /locus_tag="PKJ16_12355"
     CDS             complement(13..921)
                     /locus_tag="PKJ16_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47825.1"
                     /translation="MGKFKFFLFLLVLGGCAAYVYLFSIVHPDNSRFAIVIDNERSEV
                     IRVVGPGYTFEKQGLFPKRISWLYFPRKSSERVEIRVPIHNLEEIGNDRYAIVLPLNI
                     HYEIILSDLSVNVFRGTDVRKAFSSHIARVLTGVLTKELYRYCVPTYNRDLLARESEA
                     VVSRAIEIARDECRKSGIALTEASLLGIVRLPELAVYADGLKYLQELRDVERANAREL
                     LQLQGKIEREKIDTEQYVKRLERIAGLVKANPDLLRYIYIDRIADKVKVLVVPERTGM
                     PFGLDFDQGPSTPRPPAKKGEIDNLR"
     gene            complement(931..1167)
                     /gene="hfq"
                     /locus_tag="PKJ16_12360"
     CDS             complement(931..1167)
                     /gene="hfq"
                     /locus_tag="PKJ16_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HNV47826.1"
                     /translation="MTKNSKNLQDHYLNLARKDKVDITVYLMNGVPIKGRVLSFDNFT
                     ILIEVDKKHNLIYKHAVSTIMPLKPLKYRDDDEE"
     gene            complement(1280..2185)
                     /gene="miaA"
                     /locus_tag="PKJ16_12365"
     CDS             complement(1280..2185)
                     /gene="miaA"
                     /locus_tag="PKJ16_12365"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HNV47827.1"
                     /translation="MVGPTAAGKTDLSISLAGDVFEIVSADSVQVYRLLDIGSGKPPI
                     DVRERIPHHLIDIVSPDYHFTAGDFCVRADEACADIAGRGRVPLFVGGTGLYISSFFF
                     GLSDIPPVASAVKEQVAHDIAAHGLASMYDELAACDPLFAARIHPNDRQRIARGLEVY
                     RGLGRPLSGFYATRNARVSDDTLFVGLSVERQPLRERISARVGRMMADGFLDEVERLR
                     ALGYGSSLNSMRSIGYAQLNEHLDGACGLDDAVGRIIIATAHYAKRQMTWFGRMPQVR
                     WFSGEDQDGVRRCVDDWLQRAGPAG"
     gene            complement(2229..2435)
                     /locus_tag="PKJ16_12370"
     CDS             complement(2229..2435)
                     /locus_tag="PKJ16_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47828.1"
                     /translation="MIIPLKQLLAFNTNRYILAKAMMQAVEKTGNMKDYPTDDSAWKI
                     VPNVLKLVLGKSVNYTVEEADDEE"
     gene            complement(2444..3040)
                     /gene="gmk"
                     /locus_tag="PKJ16_12375"
     CDS             complement(2444..3040)
                     /gene="gmk"
                     /locus_tag="PKJ16_12375"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HNV47829.1"
                     /translation="MPNNISIVVSAPSGTGKTSIITKAIEENDRFVFVVSTTTRPQRN
                     GESEGESYYYVSQDDFKQMIVRGEFLEWAVVHQNYYGTSKKEVDRIKGLGKIPIFDVD
                     VQGAKQLKGKIKNAVFIFIVPPSIDELRTRLVNRGTDSEHQVSIRLNNAVNEMRDYRI
                     YDYIIVNHDLTEAAEDFKAIIRAERCNVKRFAPDMIRL"
     gene            complement(3030..3281)
                     /locus_tag="PKJ16_12380"
     CDS             complement(3030..3281)
                     /locus_tag="PKJ16_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HNV47830.1"
                     /translation="MSTKTSLINIGFGNAVVAERVIAVITPLSASGKRIREEAKDNNL
                     LIDATHGRKTRAIVVMDSSHVILSAMQPETIAGRLNNAE"
     gene            complement(3307..4173)
                     /locus_tag="PKJ16_12385"
     CDS             complement(3307..4173)
                     /locus_tag="PKJ16_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HNV47831.1"
                     /translation="MESMTGYASLERTTDQFSYAISLKTLNSKYVEVYVNVPKVLKDH
                     ENEFAQLLKKHFARGKIELSIEFFDWVDSRPSSIDSRQLGNYFRELQKINRTLKADIP
                     VTYDVLLTLDGVMRRKKTTVSEQSLRDIRRSLDAVVKKTMAMRLKEGAATAKNVLASV
                     SVIVDTLKKIRLLAKDVSRAQFERLRKSISDLTGGAADTNRLYAEVAILSDKLDINEE
                     ISRLDDHIAKFRQTVKSGGQVGRTLDFLSQEMFREINTIASKANSSTVSHHAVEVKNH
                     IDKIREQCRNIV"
     gene            complement(4215..4697)
                     /locus_tag="PKJ16_12390"
     CDS             complement(4215..4697)
                     /locus_tag="PKJ16_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002582031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV47832.1"
                     /translation="MQFKLINVGIADLKVVSTPNILRTILGSCIGICLYDPSAKIAGL
                     AHIMLPSVNASREANHRKYADSAIPILVEEMQKGGARRDRLIAKITGGATMLKMMRES
                     MMSEIGRNNAAKVREVLKEMNIPIVAENVGGDYGRTIDFFAETGMVKIKSIDRPEIDL
                     "
     gene            complement(4687..5550)
                     /locus_tag="PKJ16_12395"
     CDS             complement(4687..5550)
                     /locus_tag="PKJ16_12395"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HNV47833.1"
                     /translation="MIDFESIKRLTPEEFDRFAKLIYRESGIFLKETKLTLLSNRLRK
                     RLKALSIDTFTEYYDYIDGLADKSKEIEALIDVVSTNETYFFRNERQFDALTMHCFPE
                     FARIKGGDRTLRIWSAGCSTGEEPYTIGICVLECLHLFPGWKIEIIATDIAPSVLEFA
                     REGHYSGRRIEKVPPELLKKYFTPDRENPEVRIIHESVKKLVTFSYLNLFKTPYPQRC
                     DVIFCRNVMIYFDREHQKTLVEGFHSALQPYGYLFIGHSETLHSISDKFEYRKIEDSP
                     VYVKKGSGHAV"
     gene            complement(5580..6410)
                     /locus_tag="PKJ16_12400"
     CDS             complement(5580..6410)
                     /locus_tag="PKJ16_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47834.1"
                     /translation="MELLVMIVVNIFVCTVFYLVISLKLEKKASEFREKRIMKIMDDI
                     IRDFNLTAERNISILENRIAVMKKMLEHAGALPGIDLSVADDDAAAPVERTVDVRSSR
                     DGEERPNGGVRVASPAVSGHTSDGAGLGGMLGALVRAIGAVAARVIGGRDARADGPEE
                     RTGMRSSVSVESRVSPTAREAVARRSIDITIEKDMAELAALAADGEKAERARPKSDLL
                     PDEELATMFDTSDDKYALISDLYGKGYDLEVLSRCSGVPLGEIKLVVSLNRPTAGPTL
                     "
     gene            complement(6447..6767)
                     /locus_tag="PKJ16_12405"
     CDS             complement(6447..6767)
                     /locus_tag="PKJ16_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47835.1"
                     /translation="MSIKPIDLQTNMGQIHEISKGEKMRTDGLVEQQHLLEKESNEQS
                     RLSKSKVEENKKADSAIILRDDQSKRRRREEREKRKRQQDESSTTASTMRDERLGKII
                     DIKK"
     gene            complement(6777..8753)
                     /locus_tag="PKJ16_12410"
     CDS             complement(6777..8753)
                     /locus_tag="PKJ16_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNV47836.1"
                     /translation="MSKLKDLMQAIGDDEGQDDEIEVYADSVKNALELAARELKMDIS
                     VLDYEILEKGTSGFFGIGRQPYRVLVRPGHQYHEHDDIDKIEKKLASLSTDDLVSSRI
                     ERDADGRFSVRVTKSGVWLTVHPPVGAGKSVSLGDVQTRLYALRIKGVDEQKVEKEVK
                     KQSGKKIRLGDWIPNPEYDGTMSIEVSDDEMSAYVHFIPPQYSGRHMEFEEVMTALRN
                     AGVLVGIREKDINEYLEKMDYAQPLLAAKGDPPRDGRNAYIDYKVRVEKTDVQFEEDE
                     KGQVDFRNLDLLENVVVGQVLAVKVPFEDGIPGRTVTNRVLPAKSGKDTQIKYGKNTI
                     LSDDGNELTAATNGQVIFKAGKISVDEVLYIKGDVSLETGNITSLGSVVISGSVLDNF
                     VVKAAGNIEVRGSVQKANIEAEGDIVVKQGIMGRDEARIESTGGSVFAKFMQRANVIA
                     EKDVVAYEGIVHSNVDAGRRILCSGRRARIVGGVIRAGEEVNARFIGSESFTKTELRV
                     GVNPKVLQQVSDLEGIKKQIVDELDKLNKDYTTLTSQRKSMGGKLPKEKEELFVKMTA
                     QQKKLNARQAELDIELEELKSYLGMLGKKGKVCAEKSVFPGVDIFIKNNRFPVKDPYS
                     FVTIALEGNDVRLSQYTYPEGFEDRNTRRRPPRR"
     gene            complement(8758..>9048)
                     /locus_tag="PKJ16_12415"
     CDS             complement(8758..>9048)
                     /locus_tag="PKJ16_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV47837.1"
                     /translation="DDSDELSILETLEAPENMNPDILIEKEEIRDYIIEAIKKLPDKE
                     KKVIVLYYYEDLTLKEIGEVLEVTESRVSQLHTKAIMRLRGRLGRIKSNIMG"
BASE COUNT         1664 a         2822 c         2511 g         2051 t
ORIGIN      
        1 accccggatc gcttatcgga ggttgtcgat ctcacccttt ttcgccggcg gacggggggt
       61 cgatggcccc tggtcaaaat cgagtccgaa cggcatgccg gtccgttccg ggaccaccaa
      121 taccttcacc ttgtccgcga tccggtctat gtagatatac ctgagcaggt ccggattggc
      181 cttcaccagt ccggcgatcc gctcgaggcg cttgacgtat tgttcggtgt cgattttctc
      241 gcgctcgatc ttcccctgca gctgcagcag ctcacgggca ttcgcccgct cgacatcgcg
      301 aagctcctga aggtacttca agccgtcggc gtataccgca agctcaggaa gccgaacgat
      361 gcccaacagg ctggcctcgg taagcgcaat gccgcttttc cggcactcgt cccgtgcgat
      421 ctcgatcgcg cgggaaacca ccgcttcgct ctcgcgcgcg agcagatcgc gattgtacgt
      481 cggcacgcag taccggtaca gctccttcgt caacacaccc gtgagaacgc gcgcgatgtg
      541 cgacgagaac gcctttcgca cgtccgtgcc gcggaacacg ttaaccgaca gatcggagag
      601 aatgatctcg tagtggatat tgagcggcag cacaatggca taccggtcgt ttccgatttc
      661 ctcgaggttg tggatgggaa cgcgaatctc gactcgctcc gacgatttcc gcggaaaata
      721 cagccacgag atgcgcttgg gaaacaatcc ctgtttctcg aaggtatagc cgggaccgac
      781 aacgcggatc acctcgctcc gctcattgtc gatcacgatg gcaaagcgcg agttgtccgg
      841 gtgcacgatg ctaaacaggt acacgtacgc cgcgcacccc ccgagtacca gcagaaacag
      901 gaagaacttg aatttcccca tacagacctt ctattcttcg tcgtcgtcgc gatatttcaa
      961 cggcttcaac ggcatgattg tcgatacggc atgcttgtaa atcaaattgt gctttttgtc
     1021 aacctcaatt aatatggtaa aattgtcgaa gctgagcacg cgccccttga tgggaacgcc
     1081 gttcatgagg tacacggtga tatcgacctt gtccttgcgg gcaaggttga gataatggtc
     1141 ctgcaaattc ttcgagttct ttgtcataca aaccaccttt tattttcttt tcacacacac
     1201 agtgcgtccg ccgacaaaca agcgcggaaa gcacctacat cctacacgcc tgttcgtctg
     1261 tcaagtaaaa gtacccgttt tacccggcgg gaccggcgcg ctggagccag tcgtccacac
     1321 atcgtcgcac gccgtcctga tcttcgccgg aaaaccagcg cacctgcggc atgcggccga
     1381 accaggtcat ctgccgctta gcgtaatgcg cggtcgcgat gatgattcgc ccaaccgcat
     1441 catcgaggcc gcacgccccg tcgagatgct cattgagctg cgcgtaccca atcgagcgca
     1501 tcgaattgag cgaactcccg tacccgaggg ccctgagccg ctccacctcg tcgaggaaac
     1561 cgtccgccat catgcgcccg acccgcgcgc tgatccgctc cctgagcggt tgtcgctcga
     1621 ccgaaagccc cacgaacagc gtatcatccg atacgcgggc gtttctggtg gcgtaaaatc
     1681 cgctcaacgg gcgcccgagt ccgcgataga cctccagacc gcgcgcgatg cgctgacggt
     1741 cattcggatg gatccgcgcc gcgaagagcg gatcgcaggc ggcgagttcg tcgtacatcg
     1801 acgcgagacc gtgcgcggcg atgtcgtgcg ccacctgttc tttcaccgct gatgcgaccg
     1861 gcgggatgtc cgagagcccg aagaagaacg agctgatata cagccccgtc cctcccacga
     1921 acagcgggac ccgaccgcga ccggcgatgt cggcgcacgc ctcatcggcg cggacgcaga
     1981 aatcaccggc cgtgaaatga taatcagggg aaacaatgtc gatgagatgg tgcggaatgc
     2041 gctcccggac atcgatcggc ggtttgccgc tcccgatatc caggaggcgg tagacctgca
     2101 ccgaatcggc cgagacgatt tcaaagacgt cgcctgcaag cgagatcgaa aggtccgttt
     2161 tgcccgcggc ggtgggcccg acgataacga cggccttaat cgcgaatagc ccgtttcata
     2221 ggctggggct actcctcgtc gtcggcttcc tcgaccgtgt agttcaccga ttttccaagt
     2281 acaagcttca gcacattcgg cacgatcttc cacgcgctgt cgtcggtcgg gtagtccttc
     2341 atgttcccgg tcttctcgac cgcctgcatc atcgccttgg cgaggatata ccggttggtg
     2401 ttaaacgcaa gcaactgttt gagcgggatg atcattgtgc ctcctagagt cgtatcatgt
     2461 cgggcgcgaa tcgtttcacg ttgcagcgtt ccgctcggat aatcgccttg aagtcctcgg
     2521 cggcctcggt aagatcatga ttgacgatga tataatcgta gatccggtaa tcgcgcatct
     2581 cgttgacggc gttgttgagc cggatcgaaa cctgatgttc ggaatccgtc ccgcgattga
     2641 caagccgcgt gcgcagctcg tcgatggacg gcggaacgat gaagataaac acggcgtttt
     2701 taatcttgcc cttgagctgc ttcgcgccct gcacatcgac atcaaaaatg gggattttcc
     2761 ccaggccctt tatcctgtca acctcttttt tactggtgcc atagtagttc tgatgcacca
     2821 ccgcccactc cagaaactcg ccgcgcacaa tcatctgctt gaagtcgtcc tgcgaaacat
     2881 agtaatagct ttcgccctcg gactcgccgt tgcgctgcgg gcgggtcgtc gtcgacacca
     2941 cgaagacgaa gcggtcgttc tcctctatgg ccttcgtgat tatcgatgtc ttccccgtcc
     3001 ccgacggggc cgagaccacg attgaaatgt tattcggcat tgttcaaccg gccggcgatg
     3061 gtttccggct gcatggcgga gaggatgacg tggctcgaat ccatgaccac gatcgcgcgg
     3121 gttttgcgtc cgtgcgtggc gtcgatgagc aggttgttgt ccttcgcctc ctcgcggatg
     3181 cgcttgcccg aggccgacag cggggtgatc acggcgatca cccgctcggc gaccacggcg
     3241 ttcccgaacc cgatgttgat cagcgatgtt ttcgtagaca tggcgtccgt ctcccgtgcc
     3301 gggggcctat acgatgttcc ggcactgctc acgtattttg tcgatatggt ttttcacctc
     3361 gaccgcgtga tgcgataccg tcgagctgtt cgccttcgac gcgattgtgt tgatctcccg
     3421 gaacatctcc tgcgatagaa aatccagcgt gcgccccacc tggcccccgc tcttgacggt
     3481 ctgccggaac ttggcgatat ggtcgtcgag ccgcgagatc tcctcgttta tatccagctt
     3541 gtcggaaagg atcgcgacct ccgcgtacag gcggttggtg tcggcggcgc cgccggtgag
     3601 gtccgatatc gacttccgca accgttcgaa ctgcgcgcgg gaaacgtcct tcgcgagcag
     3661 gcggatcttc ttgagcgtat ccacgataac gctcacggac gcgagcacgt tcttcgcggt
     3721 cgcggcgccc tctttgagcc gcatcgccat cgtctttttc acgaccgcat cgagcgaacg
     3781 ccggatatcc cgaagcgact gttcggacac ggtcgtcttc ttccgccgca tcactccgtc
     3841 gagcgtcagc agcacatcgt aggtcaccgg gatgtccgcc ttcagcgtac ggttgatctt
     3901 ctgcagttcc cggaagtaat tccccagctg ccggctgtcg atggacgacg gccgcgaatc
     3961 gacccaatcg aagaactcga ttgaaagttc gatcttgccc cgagcgaagt gtttcttgag
     4021 cagctgcgcg aactcgttct cgtggtcctt gaggaccttg gggacgttca cgtacacctc
     4081 gacatatttc gaattgaggg tcttgaggct gatcgcatac gagaactggt ccgtggtcct
     4141 ctcgagcgac gcgtagccgg tcatgctttc cattgctgag tctccgtgaa atggtccgtt
     4201 ccgacgtccg cgagctagag gtcaatctcg ggccggtcga tcgatttgat cttcaccatg
     4261 ccggtctcgg cgaaaaagtc tatcgtgcgc ccgtaatcgc cgcccacgtt ctcggcgacg
     4321 atggggatgt tcatttcctt gagcacctcg cggaccttgg ccgcattgtt gcgcccgatc
     4381 tcgctcatca tgctctcgcg catcattttg agcatggtcg cgccgccggt gatcttggcg
     4441 ataagccggt cgcggcgagc gccgcccttc tgcatctctt cgaccaggat cggtatggcg
     4501 gaatccgcgt acttgcggtg gttcgcctcg cgggacgcat tgacggacgg cagcatgata
     4561 tgggcaagcc ccgcgatctt cgcggaggga tcgtataggc atatcccgat gcacgatccg
     4621 agtatggtgc gcaggatatt gggcgtcgag acgaccttga ggtcggcgat acccacattg
     4681 atgagcttaa actgcatgcc cgcttccttt tttcacgtag accggcgaat cctcgatttt
     4741 tctatactcg aacttgtccg atatcgaatg cagggtctcg gaatgaccga taaacagata
     4801 cccgtacggc tgcaacgcgg aatgaaaccc ctccaccagc gtcttctggt gctcccggtc
     4861 gaaatagatc atcacgttgc ggcaaaagat gacgtcgcag cgctgcggat agggtgtctt
     4921 gaacaggttc aggtagctga aggttaccag cttcttcacg gattcatgta tgatgcgtac
     4981 ttcagggttt tcccggtccg gtgtaaagta tttttttaac agctccggcg ggaccttttc
     5041 aatgcgccga ccggaatagt gcccctcacg ggcgaattca aggaccgatg gggcgatatc
     5101 ggtcgcaatg atctcgatct tccagccggg aaagagatgc aggcattcaa ggacgcagat
     5161 tccgatcgta tagggctcct cgccggtcga gcaccccgcg ctccagatcc gcagggtgcg
     5221 gtcgcccccc ttgatgcgcg caaactcggg aaagcaatgc atcgtcagcg cgtcgaactg
     5281 ccgttcgttc cgaaagaagt acgtttcgtt tgtggagacg acgtcgatga gcgcttcgat
     5341 ctcctttgac ttgtccgcca gcccgtcgat ataatcgtaa tactccgtga aggtgtcaat
     5401 cgacagcgcc ttgaggcgct ttcgaaggcg gttcgaaagg agcgtaagct tggtttcctt
     5461 caggaagatg ccgctctcgc ggtagatcag cttcgcgaac cggtcgaact cctcgggcgt
     5521 caggcgtttt atgctctcaa aatcaatcat cgtgcgcgtt cctatccatg tacggcgtat
     5581 tacagcgtcg gaccggccgt cggccggttg aggctcacga caagctttat ctcgcccagc
     5641 ggaacgccgg aacatcggga caggacctcg agatcgtagc cctttccgta gagatcggag
     5701 atgagcgcgt acttgtcgtc agacgtatcg aacatcgtcg ccagctcctc atcgggaagg
     5761 aggtctgatt tcggacgcgc acgctccgct ttctctccat ccgcggcgag cgctgcgagc
     5821 tcggccatgt ccttctcgat cgtaatatcg atggaccggc gcgcgacggc ctcccgggcg
     5881 gtcggtgata cgcgtgattc gaccgacacc gaagagcgca tgcccgtgcg ctcctctggt
     5941 ccgtcagcgc gcgcgtcccg tccgccgata acccgtgcgg caaccgcgcc tatcgcgcgc
     6001 actaacgcac cgagcattcc gccgaggccc gcaccatcgg acgtatgccc cgataccgcc
     6061 gggctcgcga cacgaacgcc gccattcggg cgctcctcgc catcgcgcga tgaacgtacg
     6121 tcgaccgtcc tttccacagg ggctgccgcg tcatcgtcgg caacgctgag gtcgatcccc
     6181 ggcagcgcgc cggcgtgctc gagcatcttt ttcatcacgg cgatccggtt ctccaggatc
     6241 gatatgttcc gctcggcggt gaggttgaaa tcacgaatga tgtcgtccat gatcttcatg
     6301 atccgcttct cgcggaactc cgacgccttc ttctcgagct tgaggctgat caccagatag
     6361 aaaaccgtgc acacgaaaat attcacgacg atcatcacca gcagttccat gccgccccag
     6421 tgcctccggc gccgatcccg gcgcgtctat ttcttgatat cgattatctt cccgaggcgc
     6481 tcatcgcgca tcgtcgaggc cgtcgtcgat gattcatcct gctggcgctt ccgcttctcc
     6541 cgctcctcgc ggcggcgacg cttgctctgg tcatcgcgca atatgatcgc gctgtcggcc
     6601 ttcttgtttt cctcgacctt cgacttggaa agccgggact gctcgttcga ttccttttcc
     6661 agaaggtgct gctgctccac caggccgtcg gtccgcatct tctcgccctt ggagatctca
     6721 tggatctgcc ccatgttcgt ctggaggtcg atcggtttta tcgacatggc tccacctcag
     6781 cggcgcggcg gtctccgacg ggtgttgcgg tcctcaaagc cctccgggta cgtatactgc
     6841 gagagccgta catcattgcc ctcgagcgcg atcgtcacaa agctgtaggg gtccttgacc
     6901 ggaaaacggt tgtttttaat gaagatatcg acccccggaa aaacgctctt ctcggcgcac
     6961 accttgccct ttttgccgag catgcccaga tacgacttca gctcctcgag ctcgatgtcg
     7021 agctcggcct gccgcgcgtt cagctttttc tgctgcgcgg tcatcttgac gaacagctcc
     7081 tccttctcct tcggcagctt gccgcccatg ctctttcgct gcgaggtgag cgtcgtgtag
     7141 tccttgttca gcttgtcgag ctcatcgacg atctgcttct tgatgccctc caggtcggaa
     7201 acctgctgca ggaccttggg attgacccca acccggagct ccgtcttggt gaacgattcg
     7261 gaaccgatga accgggcgtt cacctcctct ccggcgcgaa tcacgccgcc cacgatccgg
     7321 gcgcgccggc cgctgcacag gatgcgacgc ccggcatcga cattcgaatg gacgataccc
     7381 tcgtaggcca ccacgtcctt ttcagcgatc acattggcgc gctgcatgaa tttcgcgaag
     7441 accgaaccgc cggtggactc gatacgagcc tcgtcgcggc ccatgatccc ctgcttcacg
     7501 acgatatcgc cctcggcctc gatgttcgcc ttttgcacgg agccgcgcac ctcgatgttg
     7561 ccggcggcct tcacgacgaa gttgtcgagc acgctccctg agatcaccac cgaccccagg
     7621 ctggtgatat tgccggtctc cagcgagacg tcgcccttga tgtataggac ctcgtcaacc
     7681 gagatctttc ccgccttgaa aatgacctgc ccgttggtgg ccgccgtcag ttcgttcccg
     7741 tcgtcggaga ggattgtgtt tttcccgtac ttgatctgcg tatccttgcc cgacttcgcc
     7801 ggcaggacgc gattggtgac cgtccgcccg ggtatcccgt cctcgaacgg gaccttgacg
     7861 gcaagcacct gccctacgac cacgttctcg agcaagtcca ggttgcggaa gtcgacctga
     7921 cccttctcgt cctcttcgaa ctgcacatcg gtcttttcga cccggacctt gtaatcgatg
     7981 taggcattgc gcccgtcgcg gggcggatca cccttggccg ccaggagcgg ctgcgcatag
     8041 tccatcttct cgaggtattc gttgatgtcc ttctcgcgga tgcccacgag caccccggca
     8101 ttgcgcagcg ccgtcatcac ctcctcgaac tccatgtgcc gccccgaata ctgcgggggg
     8161 ataaaatgaa catacgccga catctcgtcg tcggagacct cgatgctcat ggtgccgtca
     8221 tattcgggat tcgggatcca atctcccagc ctgatctttt tgccggactg tttttttacc
     8281 tctttctcga ccttctgttc gtcgacgccc ttgatgcgca gcgcgtacag gcgcgtttgc
     8341 acgtcgccca ggctcaccga tttccccgcg cccaccggcg ggtggacggt gagccagacg
     8401 cccgacttgg tcacgcgcac gctgaagcgc ccgtccgcgt cccgttcgat gcggctggaa
     8461 acgagatcgt cggtcgagag gctcgcgagc tttttctcga ttttgtcgat atcgtcgtgt
     8521 tcgtgatatt gatggcccgg ccggacaagc acccggtagg gctggcgccc aatgccgaag
     8581 aatccgctag tccccttctc cagtatttcg taatcgagga ccgagatgtc catcttgagt
     8641 tcgcgggctg caagctcgag cgcgtttttc accgaatcgg catagacctc gatctcgtcg
     8701 tcttggccct cgtcgtcccc tatggcctgc atgaggtctt tcagcttgct catgcgctca
     8761 tcccatgatg ttcgacttga tccgtccgag acgaccgcgc agccgcatga tggccttggt
     8821 gtggagctgc gaaacgcggc tctccgtgac ctcgagcacc tcgccgattt ccttgagcgt
     8881 gaggtcctcg taatagtaca ggacaatcac cttcttctcc ttgtcgggga gctttttgat
     8941 ggcctcgatg atatagtctc ggatctcctc cttctcgatg aggatatcgg gattcatgtt
     9001 ctccggcgct tccagggtct ccaagatgga gagctcgtcg ctgtcgtc
//
LOCUS       DAORHF010000152         8922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7270_length_8922_cov_5.108492, whole genome shotgun sequence.
ACCESSION   DAORHF010000152 DAORHF010000000
VERSION     DAORHF010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7270_length_8922_cov_5.108492"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(69..980)
                     /locus_tag="PKJ16_12420"
     CDS             complement(69..980)
                     /locus_tag="PKJ16_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47838.1"
                     /translation="MNTISSIDVTAYYQQSPRKMFRQARSLFRKNRIHDAIAILKALL
                     KQGHDPVSVQGMIAQCYDRLSFLTRDIECEDLAKLTYEKLLGMDLKRRQKRRIQKEYD
                     RFINRIYELGEPDYKAFMKARQLRNREIKTPKAWMMLGSNFNIRKDVDFVINAYKNAL
                     ELDPKYILALYKTGYVLQYNKQDADAALPYYVRVIKTDPESDENESLTTNARCILDAC
                     NQLGKLYYARGEYRKVVAVFNHALKTQAAYLPQGVLLSIRELIYLTDHSSTQLNLKER
                     LDGHLRAHFDLTLTLMVQKYCKNISVA"
     gene            complement(1090..1554)
                     /locus_tag="PKJ16_12425"
     CDS             complement(1090..1554)
                     /locus_tag="PKJ16_12425"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV47839.1"
                     /translation="MYTIRTASLRDIDDVVDLRLKLLEELGIVNSGDDSIRFRESTRG
                     YFIKKIQTSDFKAWVADSGSDIVSMTSIQFIDHPPVKELAHAIDAHVMDVYTIKDMRG
                     KGVDAELLARVIEYAKNNNAQRVVINTIGRDTRIYAKLGFVQTTTDMELALK"
     gene            2295..2969
                     /locus_tag="PKJ16_12430"
     CDS             2295..2969
                     /locus_tag="PKJ16_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47840.1"
                     /translation="MGRPHVVGFSFLAPDVYAAVSGFSDFAVAIRIAISILLVMVAVA
                     VAVLGALFLHKFNALITALARSLKDAAAGNPPLMNPQADTELYELAHSFNSLAGEIAG
                     LKRDPVQQQVHDGSDAQVSSPSGLDEAFRAGVASLKDNELDRAIGVFQALIAINPDSF
                     GSYFNCGVALAKKRIYDEALAMFRTAQEKNPDNELVARYIDRVSRLHDSTNATEPDAI
                     DAPKQE"
     gene            2926..4791
                     /gene="uvrC"
                     /locus_tag="PKJ16_12435"
     CDS             2926..4791
                     /gene="uvrC"
                     /locus_tag="PKJ16_12435"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HNV47841.1"
                     /translation="MQPNQTPSTLRSKSRNLPRQPGVYIMKSANGAIIYVGKATSLRA
                     RVSSYFSGSLPDRKTELLVRAIDDFEYIVTDSEIEALILENTLIKKHQPKYNVRLRDD
                     KRYPYIAVTLSEEYPRLVFTRKLQDRRSRYFGPYTDAKAARTIVETMNRLFKLRRCAR
                     SLPLRPGERPCLNFQMKRCSGVCAGTLSRDDYRAVIDVVIRFMEGDHAPVIEQLQEQM
                     RAYSSRFEYEKASYLRDVIFDIQRFTDTQKVVTSIGHDRDYIGIGAGGDGDEAVAIVF
                     EFRNGVLIGRKIHVFDNALQAPSRDILRTLIVDCYAHDDTAQSRTSATMPSRIVVAER
                     IPDSALLERHLSGIAKKSITISTPRTADDASIMNMIAKNIDVIIAERAAHRVQSDTAA
                     RLIELRDALQLAREPEIIECFDISNLQGTHAVASMVQFKNGAPNTRGYRRYKIRGYDG
                     ANDPGMIHEAVARRLQYLMNESIPPPDVLVVDGGATQVARAREAADALGCAVTIIGLA
                     KRHEEIHFPDDRRPLRLPPRSHARKLLQAIRDEAHRFAHDYHTKLRGKAMTASSLDEI
                     PGIASGTKRLLLSHFADVEAIRRASTDELCAVPGIGKKIAEKIHRHLHAETKGTV"
     gene            complement(4788..5774)
                     /locus_tag="PKJ16_12440"
     CDS             complement(4788..5774)
                     /locus_tag="PKJ16_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HNV47842.1"
                     /translation="MIVLNFDAPDWISVRLSIALSLAMRKPVTLPGAARVLDDTPHAR
                     ALIGDIETFLSCAGCGSIAVEEDAIIFRPETIPGGRYRIASGPCASAVELLLAMAPAL
                     SMRDFQSRLTIRGVTHASQSHPTVFLNETLFQFTDALGFPFFSALVRYGFFGTGNGSL
                     DAKIYPRTHTNDFSRFDEAVCSPTLALAGARITVSKLPGDIAREEKRILSEGFGTPDE
                     RVSIIEVMDAAGPGHSVNAYLHSAIGAIVVTDTFDFFDATGERIVDPARLGFDLQPFV
                     DSVRAIIDTQRLPDFLLRELVPYCIIAGHTAPGMFENAPDAARDFAALQKLA"
     gene            complement(5778..7040)
                     /gene="serS"
                     /locus_tag="PKJ16_12445"
     CDS             complement(5778..7040)
                     /gene="serS"
                     /locus_tag="PKJ16_12445"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HNV47843.1"
                     /translation="MINPKYIREDMALVRRMLTIRNMEQAVDLGRLEALDAERRALVT
                     RSDQLREQRNKLSKEIGARKAKKENADDLMREVDGIAADIKDVNAKVEALEGEYNDLV
                     LSVPNILDGSVPEGGGEEDNVVVRTWGEKPVFDFTPKPHFEIGVDMNILDFERGVKIA
                     GTRFYVYRDLAARLERAIINFMLDLHTTEHGYTEVFGPYIVNDDSMIGTGQFPKFKDE
                     YYRIERDGLSLIPTAEVTLTNLYRDEIIEKEDLPVYVTMQSACFRREAGSAGKDTRGL
                     VRVHQFQKVELVKFVDPETSFDELESLVASAEEVLRRLNLHYRVLLLCSADTSASSAK
                     TYDIEVWMPGLDRYVEISSCSNFIDYQARRARIRYRPVKGGKPEFLHTLNGSGLAAGR
                     TLAAVMENYQTKDGGLAIPDALKKYLAK"
     gene            7156..>8922
                     /locus_tag="PKJ16_12450"
     CDS             7156..>8922
                     /locus_tag="PKJ16_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HNV47844.1"
                     /translation="MVSDHHKIIDALERSHPEKFLPADAAFARIKRGSRIFMGTGCGQ
                     PRFLLNALIDYAEANPKALFDAEMYVLLAFGMEQYRYERFERSFHQHCFTFRDNTRRT
                     LNRGSGDYNPVFLSNIPRFIKRRIIPIDVAFVQVSLPDVRGRVSLGISVDITRSAVDA
                     AGLVIAQMNAHMPFVHGDAVLPIEAFDVIIPHDEPLLEIEPFERDDTIDAIGAFVSGL
                     VEDGSTLSVGHGRTAQGIIPHLAGKKNLGIHSELISDNLVGLIRAGAVDNAHRSIHPG
                     VSTGTICMGSAETYRFLHDNRSIELKTADYVNNPAVIANNRAMVSINGVLSVDCTGQA
                     TAESIGASFYSGLGGTSDFARGASLSDDGKSILVLRSSAQGGAVSRIVPFLKEGEGIT
                     VNRGDAAYVVTEYGVAHLAGKNIRNRALELIGIAHPAHRAWLIEQAKRHNLIHRDQVF
                     IPGKSGEYPVQLEETVSLDERCELLIRPVKLSDERLLKEFFYSLSPQSLYNRFVTVQK
                     EMPHEVLQTLVAIDYRQQMTLLAIDRTDERETVLGIAQYIVEDPHFANFSISILDDWQ
                     NRGIGSILLDRCVAIARQLGISG"
BASE COUNT         1829 a         2664 c         2562 g         1867 t
ORIGIN      
        1 caccgagagt gcgagcgcca gcagcagacc gaacgtgagc ggtaccttgg ggatcatgac
       61 gctcatgctc aagcgaccga tatgttttta cagtatttct gcaccatcag cgtgagggtg
      121 aggtcgaaat gtgcgcggag atgtccatcg agccgctcct tgaggttgag ctgcgtcgaa
      181 gaatggtcgg taagatagat gagctcgcgt atcgacaaga ggacgccctg cggaaggtac
      241 gcagcctgcg tcttcaaagc gtgattgaac acggcgacga ccttccggta ctctccgcgg
      301 gcatagtaca gcttccccag ctgattgcag gcgtcgagaa tacatcgggc gttcgtcgtt
      361 agagactcat tctcgtccga ctcgggatcg gtcttgatga cgcgaacgta gtaggggagc
      421 gccgcatcgg cgtcctgctt gttgtattga agcacgtacc ctgtcttgta aagagcgagg
      481 atatacttag gatcgagctc aagcgcattt ttataggcgt tgatgacgaa atcgacgtcc
      541 tttcgtatgt tgaagttcga tccgagcatc atccacgctt tgggcgtttt gatctcacgg
      601 tttctgagct gccgcgcctt catgaatgcc ttgtaatcgg gctctcccag ttcgtagata
      661 cggttgatga atctgtcgta ttccttttga atccgtcttt tctggcgacg tttcaggtcc
      721 atccccagga gcttttcata cgtgagtttc gcaaggtctt cacattcgat gtcgcgcgtc
      781 aggaaggaaa gccggtcgta acattgcgcg atcataccct ggaccgatac cggatcatgt
      841 ccctgcttca agagggcctt gagaatcgcg attgcgtcgt gaatacgatt tttcctgaag
      901 agagagcgcg cctgccggaa catctttcgc gggctttgtt gatagtaggc cgtcacatcg
      961 atgctggaga ttgtgttcaa tggttccatc agactgatac acacgtactc ggattctgcg
     1021 cgaacgggag tcgccccact tatattgtcg gctcattgcc tgatgaagcc atacaatatt
     1081 tcgtcctggc tacttgaggg cgagctccat gtccgtcgtg gtctgtacga agcccaactt
     1141 cgcataaata cgggtatccc tgccgatggt attgatcacg acccgttgcg cattgttgtt
     1201 tttggcatat tctatcactc gggcgagaag ctccgcatca acgcctttcc cgcgcatgtc
     1261 cttgatcgta tagacgtcca tgacgtgcgc atcgatggca tgcgccagtt ccttcacggg
     1321 aggatgatca atgaactgga tgctggtcat cgacacgata tcggatccgg aatccgctac
     1381 ccacgccttg aaatcgctgg tttgaatttt ttttataaag taaccgcgcg tcgattctcg
     1441 aaatcgaatc gaatcgtcgc cgctgttgac gatccccagc tcctcgagca gtttgagtcg
     1501 aagatcgacg acatcgtcga tatcgcgaag gcttgccgtt ctgatcgtat acataatcgc
     1561 ctccggtgca tgtgtgtgat cgaatcgtac atactacggt tgatgcaaaa cgggatggtc
     1621 tttcatcgcg aggggcgaca cattgcttcg atttttcccg cgatgacatc acgttacgca
     1681 actcgtctat gtatatatgc ggcaatatac acgtcaatag tattataccg gctactagaa
     1741 taaaaattac ttgaataatt catggagtca tatgatgaaa gcggacgggc agcatacgaa
     1801 tggaaccgac ggcaaaattc gggttttctc gcaggtttat ccttggcata acgctcctca
     1861 tggttgcgct tattgctgtt cttggcgtgt cgttccattt gatcgtgaac cggtgttctt
     1921 cggttctcca aacgcatttg acgacaagct cggctggtca cctcgcgcaa acctcgaagt
     1981 cgcttctcgc cgccgtgcct gaccgatcgt caatcgatcg attcatgcgg tcaattgcgg
     2041 ataatcgact ccttcatgag ggcctggtcg ccgcgtctgt tttttcgaaa accggggatg
     2101 aaaaatattt tcgcgtcgcc gcgattcgcg cattcgacga acgacactta ttaactctca
     2161 aggtcaacga tcaggtagcg gcccccggcg atagcaccct tctgcgggac gccctgattg
     2221 ctccgcagat cgatccgcat gtacatgtgc gcgggcgaac cgcatggcaa accgcttatc
     2281 tgccggttgt gatcatgggg aggccccatg tggtcggttt ttccttcctt gcgccggatg
     2341 tgtatgcggc cgtgtcaggt ttttccgatt tcgcggtcgc catccgaatc gcgatatcaa
     2401 tccttctcgt aatggtcgcc gttgccgtcg ctgttttagg cgcgctcttt cttcataaat
     2461 tcaatgcgtt gatcaccgcg ctcgcgcgat cgttgaagga cgcggccgcc ggcaatccac
     2521 cgcttatgaa cccgcaggcc gataccgaac tctacgagct cgcgcattcc ttcaacagcc
     2581 ttgccggcga gatcgccgga ctcaaacgcg accccgtcca gcagcaggtc cacgatggtt
     2641 cggacgcgca agtatcatct ccatccggac tggacgaggc atttcgcgcc ggcgtcgcgt
     2701 cattgaagga caacgagctc gatcgcgcga tcggcgtctt ccaggcgctc atcgcgatca
     2761 acccggacag cttcgggagc tatttcaact gcggcgtcgc gctcgcgaaa aagcgcatat
     2821 acgatgaggc gctggcgatg tttcgcacgg cccaggagaa aaatcccgac aacgagctcg
     2881 ttgcgcgcta cattgatcgc gtgagccgtt tacatgattc gacgaatgca accgaaccag
     2941 acgccatcga cgctccgaag caagagtagg aatcttcccc gccagcccgg cgtctatatc
     3001 atgaaaagcg ccaacggcgc aatcatatac gtcggcaagg cgacgtcgct gcgagcgcgc
     3061 gtatcgtcat atttttcagg atcgcttccc gaccgaaaga ccgagctgct ggtccgcgcc
     3121 atcgacgatt tcgaatatat cgtgaccgac tccgagatcg aagcgctgat cctcgagaac
     3181 accctcatta aaaaacacca gcccaagtac aatgttcgcc tgcgcgacga caagcgttat
     3241 ccgtacatcg ccgtaaccct gtccgaggag tatccgcgtc tcgtcttcac cagaaagctg
     3301 caggaccgac gaagtcggta cttcggaccg tacaccgacg caaaggccgc tcgcacaatc
     3361 gtcgaaacga tgaaccggct tttcaagctt cgtcgttgcg cgcgctccct cccgcttcgc
     3421 ccgggagagc ggccatgcct caatttccaa atgaagcgat gctccggcgt gtgcgcaggg
     3481 accctctcgc gtgacgatta tcgcgccgtg atcgacgtcg tgatcagatt catggagggc
     3541 gatcacgccc cggtcatcga acagctgcag gaacagatgc gggcctattc gtcccgattc
     3601 gagtacgaaa aggcgtcgta ccttcgggac gtcatctttg atatccagcg gttcaccgat
     3661 acgcagaagg tcgtcacgtc catcggccat gatcgcgatt acatcggcat cggcgcgggc
     3721 ggtgacggtg acgaggccgt cgctattgtc ttcgagttca gaaacggcgt gctcatcggg
     3781 aggaagatcc atgtcttcga caatgcgctg caggcgccgt cccgcgacat ccttcggacg
     3841 ctgatcgtcg actgttatgc gcacgacgac accgcgcaat cgcgcacgtc ggcaaccatg
     3901 ccctcgcgga tcgtcgtcgc ggaacgcatt cccgattccg cgctgctcga gcggcatctt
     3961 tcgggcattg cgaaaaagtc cattaccatc tcgacgccgc gcaccgccga cgacgcgagc
     4021 atcatgaaca tgattgcgaa gaatatcgac gtgatcatcg ccgagcgcgc ggctcatcgc
     4081 gtgcagtccg atacagcggc acgcctcatc gaactgcgcg acgctctcca gcttgcgcgc
     4141 gagccggaga tcatcgaatg ttttgacata tccaatcttc aggggacaca cgcggtcgcg
     4201 tcgatggtcc aatttaaaaa tggcgcgccc aacacccgcg ggtatcggcg atataaaatt
     4261 cgtggctacg acggggccaa cgatcccggc atgatacacg aggcggtggc caggcgcctg
     4321 cagtacctga tgaacgaatc gatcccgccg cccgacgtcc tggttgtcga cggtggggcg
     4381 acgcaggtgg cccgggcccg cgaggcggcc gatgcgctgg ggtgcgcggt gacgatcatc
     4441 ggcctggcga aacgccacga ggagattcat ttccccgacg accgacgacc gctcagactg
     4501 ccgccgcgct cgcacgcacg aaaacttctg caggcgatcc gcgacgaggc gcaccgattc
     4561 gcgcacgact accacacgaa actgcgcgga aaggccatga cggcgtcctc tctcgacgag
     4621 atcccgggta tcgcctctgg cacaaagcgt ctgctcctgt cgcattttgc cgatgtcgag
     4681 gcgatccgac gcgcctcgac cgatgagctg tgcgcggttc cgggcatcgg caaaaaaatc
     4741 gccgagaaga ttcatcgtca tcttcacgcg gaaacgaagg gcacggttta ggcgagcttc
     4801 tggagtgcgg cgaaatcgcg tgcggcatcg ggggcgtttt cgaacatgcc cggtgccgta
     4861 tggccggcga tgatacagta gggaacgagc tcccggagca ggaagtccgg caggcgttga
     4921 gtgtcgatga tggcgcgcac cgaatcaacg aacggttgaa gatcgaaccc gaggcgcgcg
     4981 ggatcgacga tgcgctcgcc ggttgcatcg aaaaagtcga aggtgtccgt gaccacgatg
     5041 gcgccgatcg cagagtgcag gtacgcattc acgctgtggc ccggccccgc ggcgtccata
     5101 acctcgatga tggaaacccg ctcatccggc gtcccaaacc cttcggacag gatgcgcttt
     5161 tcctcgcgcg caatgtcgcc gggcagcttc gaaaccgtga tgcgcgcacc cgcgagcgca
     5221 agcgtcggag agcataccgc ttcatcaaat cgcgaaaaat cgtttgtgtg cgtgcgcgga
     5281 tatatcttcg cgtctaacga cccgttcccg gttccgaaaa agccgtatcg gacaagcgct
     5341 gaaaagaacg gaaatcccag ggcatcggtg aattgaaaaa gcgtttcatt caggaaaaca
     5401 gtcgggtgag attgggacgc atgcgtaacg ccgcggatgg tgagtcggga ctggaagtcc
     5461 cgcatggaaa gcgccggggc catcgcgagt aaaagctcca cggcgctcgc gcacggcccc
     5521 gatgctatcc gatagcgacc gccgggaatc gtctccggtc tgaaaatgat cgcgtcctct
     5581 tcaacggcta ttgagccgca accggcgcag gaaaggaacg tctcgatatc gccgatgagc
     5641 gctcgtgcgt gcggcgtgtc gtcgagaacg cgtgcggcgc ccggaagcgt gaccggtttc
     5701 cgcatggcga gcgagagcgc tatcgacagg cgcaccgaaa tccagtcggg cgcgtcgaaa
     5761 ttgaggacga tcatacgcta cttcgccaga tattttttca gcgcgtccgg aatggcgagg
     5821 ccgccatcct tcgtctggta gttctccatg accgcagcga gcgttctccc ggccgcaaga
     5881 ccggagccgt tgagcgtatg caggaattcg ggttttccgc ccttgaccgg acggtagcgg
     5941 atccgcgccc gccgcgcctg gtagtcgata aaattcgagc aggaggaaat ctccacgtag
     6001 cggtcgagac ccggcatcca cacctcgata tcgtaggtct tggccgacga ggccgaggta
     6061 tccgcgctgc agagcaggag aacccgatag tgcagattga ggcgacggag gacctcctcg
     6121 gcgctggcga caagcgattc gagctcgtcg aacgatgttt ccggatcgac gaatttgacg
     6181 agctcgacct tctgaaactg gtggacccga accaggccgc gcgtgtcctt tccggcggac
     6241 ccggcctcac gccgaaagca ggccgactgc atcgtcacgt agacgggcag atcctccttc
     6301 tcgatgatct cgtcgcggta gagattggtg agcgtcacct cggcggtggg gatgagagag
     6361 agcccgtcgc gctcgatgcg atagtactcg tccttgaact tggggaactg accggtcccg
     6421 atcatgctgt cgtcgttgac gatgtaggga ccgaacacct cggtgtaacc gtgctcggtc
     6481 gtgtgcaggt ccagcatgaa gttgatgatg gcccgttcga ggcgcgccgc gagatcgcga
     6541 tagacgtaaa agcgcgtccc ggcgattttt acgccgcgct cgaaatcgag gatgttcatg
     6601 tcgacgccga tctcgaaatg cggtttcggg gtgaaatcga agacgggttt ctcgccccag
     6661 gtgcgcacga ccacattgtc ctcttccccg ccgccctcgg ggaccgatcc gtcgaggatg
     6721 ttcgggaccg acagcacgag atcgttgtac tcgccctcga gcgcctcgac cttcgcgttc
     6781 acgtccttga tgtccgcagc gattccatca acctcgcgca tgaggtcgtc cgcgttctcc
     6841 ttcttcgcct ttcgagcgcc gatctccttc gagagtttgt tgcgctgttc ccgcagctgg
     6901 tcggagcggg tcacgagggc gcgacgctcg gcgtccagcg cttcaagtcg gccaagatcc
     6961 accgcctgct ccatgtttct gattgtgagc atgcgccgca ccagcgccat gtcctcgcgg
     7021 atatatttcg ggttgatcat acgggggcct cgcgatgttt tccgcgcgcc gttaaattta
     7081 tggttgaaca aaacggtcgc acgtgtgatg gtatagcatc attacgcgga accggaccgc
     7141 attttgtcaa gaaagatggt ttcagaccac cataaaatca ttgatgcgct cgagcgctcg
     7201 cacccggaga agttcctgcc ggcggacgcg gccttcgccc gcatcaagcg gggcagccgc
     7261 atcttcatgg gcaccggttg cggacagccg agattcctgc tcaacgccct catcgactac
     7321 gcggaggcga atcccaaggc gctcttcgac gccgagatgt acgtgctcct cgccttcggc
     7381 atggaacaat accgatacga gcgcttcgag cggtcgttcc atcaacactg tttcacgttc
     7441 cgtgacaaca cccgacgcac gttgaatcgc ggaagcggcg attacaaccc ggtgtttctc
     7501 tcgaacatac cgcgcttcat caagcggcgg atcatcccga tcgatgtcgc gttcgtgcag
     7561 gtttcgcttc ccgacgttcg cggccgcgtg agcctgggca tcagcgtcga tatcacgcga
     7621 agcgccgtcg acgccgccgg tctcgtgatc gcccagatga acgcccacat gcccttcgtc
     7681 cacggcgacg ccgtgctccc gatcgaggcc ttcgacgtca tcattccgca cgacgagccg
     7741 ctccttgaga tcgagccgtt cgaacgggac gatacgatcg acgccatcgg cgccttcgtg
     7801 tcgggcctgg tcgaagacgg cagcaccctc agcgtcggac acggacggac cgcacagggg
     7861 atcatccccc acctcgccgg gaaaaaaaac ctggggatcc actcggagct catcagcgac
     7921 aacctcgtgg ggctgatccg cgcgggcgcg gtcgacaacg cccatcgatc catccacccc
     7981 ggcgtgtcca ccggcaccat ctgcatgggg agcgccgaga cctaccggtt cctgcacgac
     8041 aaccgctcca tcgaactcaa gaccgcggac tacgtgaaca atcccgcggt gatcgcgaac
     8101 aatcgcgcca tggtgtccat caacggggtc ctttcggtcg attgcaccgg acaggcgacc
     8161 gcggaatcca tcggcgcatc gttctacagc ggcctcggcg gcacgtccga tttcgcgcgg
     8221 ggcgcgtcac tgtccgacga cggaaagtcc atactggtcc tccggtcgag cgcgcagggc
     8281 ggcgccgttt ctcgcatcgt tccgttcctc aaggaggggg aggggatcac cgtgaatcgc
     8341 ggggacgccg cgtatgtcgt caccgaatac ggcgtggccc atcttgccgg gaagaatatt
     8401 cgcaatcgcg cgctcgagct catcggcatc gcccatcccg cgcaccgcgc ctggctcatc
     8461 gagcaggcca agcgtcacaa tctcatccat cgcgatcagg tcttcatccc gggaaaatcc
     8521 ggcgaatatc cggtccagct cgaggagacg gtttccctgg acgaacgatg cgagcttttg
     8581 atccgacccg tcaagctcag cgatgagcgg ctgctcaagg agttctttta ttcgctctct
     8641 ccgcaaagtc tgtacaatcg gttcgtcacg gtccagaagg agatgccgca cgaggtgctg
     8701 cagacgctcg tggcaatcga ttaccgccag cagatgacgc tccttgccat cgacagaacg
     8761 gatgagcgcg agaccgtcct cggtattgcc cagtacatcg tcgaggatcc gcacttcgcg
     8821 aatttttcta tttcgatact cgacgactgg caaaaccgcg gcatcggctc gatcctgctg
     8881 gaccggtgcg tcgcgatcgc gcgtcagctg ggcatatcgg gc
//
LOCUS       DAORHF010000153         8899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7292_length_8899_cov_5.441655, whole genome shotgun sequence.
ACCESSION   DAORHF010000153 DAORHF010000000
VERSION     DAORHF010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7292_length_8899_cov_5.441655"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PKJ16_12455"
     CDS             complement(<1..542)
                     /locus_tag="PKJ16_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47845.1"
                     /translation="MEIPIKKVFAALLGCLLAFASMGTALMAQDRNGGRTDTTKNITD
                     DDDDAADDEEADGEADNGTDAGKKKDDLFHLTLGGKGYHNSGGAPTYTYWKPFTELEL
                     TTTYFFLKAGYYRYIGYQITDGSGNYEYIGFNQYAGELGLTPFKWLEVSGGYHFSEGE
                     FRYKAHEYSGELVLDFDIITL"
     gene            complement(714..1154)
                     /locus_tag="PKJ16_12460"
     CDS             complement(714..1154)
                     /locus_tag="PKJ16_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47846.1"
                     /translation="MKKLLLMAIAMVIGVTLAAYGQDTGTTPGAGERKEVREERREVK
                     EERKEVRAAEESLKAKQEQLKTDQAKLKADLKLLGQTKDPEKKRQLKETIRQDKAAIT
                     KDKKEIREARRELRRERKELRKERKELRKERKELRKEQREKPGK"
     gene            complement(1295..3349)
                     /locus_tag="PKJ16_12465"
     CDS             complement(1295..3349)
                     /locus_tag="PKJ16_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV47847.1"
                     /translation="MSAGTSLSSLQRIFISTTIDHLIAVPDEHADSVVVSDRIIDEIT
                     KILASKKGGTLFAVIFIDIDDYEQAYALLKSFKPSDLAYRIVAMDPENRHTLSNMGRL
                     KYIHELRTTPIMDREFIFLVRKSFDAMVDEIAAAAERQSYQIRLMDAKHDQEDLISIG
                     KSLSLEKDPDKLLRSILFLSKRITGADAGSIYLVEDATGGGTQLRFKYSHTFSKEIPM
                     EEFVIPYDTNSIAGYVAITGTVLNIPDVYELSTEDPITFNSNFDRTQSYRSKSMLVVP
                     MRNHIDEIIGVIQLINSKENLDSASVTGNEAFEVRLTTLEDFETKVVPFDHRYESLME
                     AVASQAAIAIENNRMIRQIENQFEEFVRASVTAIESRDVATSGHSFRVAEICTRLAHA
                     VNQATDGPFKHVNFNENAVKELEYAALLHDFGKVYIDIAIFMKGKKLFPKEFENLILK
                     MNYLYRSIELNYSLEEAQIAVRGADPALIEEARTRKEERERTLAAIKVIIEEVRRLNE
                     PAVTVTDIERRICEIDETVRGFRCYDIDGNIVRVLTDDEKTNLGIPRGSLNESERREI
                     ESHVVHTYNFVSKIPWPPEFKRIPEIALKHHEKLDGSGYPNRLTAGDIPIQAKMMAIA
                     DIYDALTASDRPYKKALPAERALSILDDEAKHNKIDPELFRLFVEKRIYEQVGTGSVA
                     AP"
     gene            3537..4967
                     /locus_tag="PKJ16_12470"
     CDS             3537..4967
                     /locus_tag="PKJ16_12470"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HNV47848.1"
                     /translation="MKQSTRFISLATACVVILAASVSASCAAEKKKEKRARPRTLVEL
                     NRDLIELTRRTLPSVVNISAVKNIAADAPMMRFRIPDQKQPDAPRKRSSIGSGVIIDA
                     RGYIITNNHVIKNTKGIEVTLSDKRVFKCTVVGADPATDIAVIRIADPAPKDLPVAVI
                     GDSDAIRVGEIVIAIGNPFGFAQTVTMGIVSATGRQNIGLASYENFIQTDAAINPGNS
                     GGALVNVDGVLVGINTAIFSRSGGSIGIGFAIPAKMVKQVMSELISSGRVVRGWLGVY
                     IQDVTKDIADSIGFARKGGAMVSDIMKGSPAEGSGVAVGDIIVSVNGGEIRDVDQLRK
                     LIAALKPDSVITLGVFRGGKTSEVKIKIGAMPDEPQRADTRKTEADDELGMAVRDVDE
                     ALAYKYKVADGRGVVIVAVKAGSPAGKAGLIEGDLIKEVDQRPVGTAAAYRSVLATLK
                     TRTKVLLLVKRQGMNKFVVINRGPEE"
     gene            4984..8523
                     /locus_tag="PKJ16_12475"
     CDS             4984..8523
                     /locus_tag="PKJ16_12475"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47849.1"
                     /translation="MGHAEEKYRRIIESLREGYYEVALDGTFTFVNDAVCGFLGRSRA
                     DVLGTRYRDHLPADIAGGVDAVLESVRVSERRRDLVRIPLRRIDGSTVQTEHSIDVVR
                     DDSGAVAGFRGVARDITGRLRAELELRASEEKFRQLVENIEDVIYIVNPAGRIRYISP
                     SGLRRIGHSEEDLAARPFFEFIQEEHRAAAVAFYRDQLSRNVSKTFYELPIIKKDGSV
                     IWVEQSVKLVPTEGGGTEFYCIARDITEKKKAVEALRRSEERYRNILGSINDGYYEVD
                     LAGAITFCNEAMARTLGYPPEELVSMGYGHFMDESEINIVYSIFNEVYQTGVPSEVFA
                     YKFRRKDGSVCIAETTASLVFDEAGRKAGFRGILRDITERKNAEDELRRSEEKYRLII
                     EHASDIICMCDRSGRFLFMNPAGLARFGYTAEGLMGIRYRELIPTAHRERVLTFYRDQ
                     LRKQIRQTYLEFEAATRDGELFWVGQTVSMVADADGALEFHIVARDITAKKQAEAALI
                     ELDRQKSNFFANVSHEIRTPLTLILSPVESALQGDFGDRADRDFLLGIQRNAIRLLKL
                     INNILDFSKIEAGRMTMRLRETDIVAFLRRYLGSIESAAEARNIALDLAAAPDALTLL
                     ADTEKMDKVFMNLFSNALKFTAGGGRITVRVRVDAGACVIEMEDDGEGIPADKTRIIF
                     DRFGQADTGTTRRYEGTGIGLALAKEFVEMHDGTISVVSRHVDAHPDDHGSVFIITLP
                     MDRERWAGRPGVEIVTGSEVEDAVADHRFAGMREMTDLAAPAPPVTASTHSPVPSDRP
                     RVLVVDDNADMREFIAALLARDYEVHLAVHGADGLARADELRPDLIVTDVMMPVMNGY
                     ELTRRLKARDDLRALPVIMLTARAELANKIEGLEFGADDYLTKPFNSKELLTRVAALL
                     KSSRFQRMLAERNRIIENDLAVARLIIDRILPRGVPGTAGFRFHATYLPADTVGGDFY
                     DIAARDGRIELFIADVSGHGLSSAFLAMIAKITLDAMPDRSSPGRVLADLNEAICRAT
                     VQANFITAFYGVVDRADGVMRYCSAGHAPALLFRRGRDGCAELNAQGKPMGWFAGEAY
                     EERRERLEPGDRVVLYTDGITEAWRADTVCDCRDPECDMFGQERLAACLRDCADRGPE
                     GFSRELMARLRAFTATERFSDDITMIVFDVTAP"
     gene            complement(8551..>8899)
                     /locus_tag="PKJ16_12480"
     CDS             complement(8551..>8899)
                     /locus_tag="PKJ16_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV47850.1"
                     /translation="LKKKVMKIVTDSTPVESPKNPETCNVFNLYRLFAPADRVENLRD
                     RYAKGGTGYGEVKKELVELIWEFFRPYRERRAELAKDPGAVRGILAAGAEKTRAVASV
                     TLREARKKVGLNY"
BASE COUNT         1480 a         2752 c         2954 g         1713 t
ORIGIN      
        1 agggtgatga tgtcgaaatc gagcaccagc tcgcctgaat actcgtgggc cttgtagcgg
       61 aattcaccct ctgagaaatg gtatccgccg ctcacctcga gccatttgaa aggggtgagc
      121 cccaattccc ctgcgtactg gttgaagccg atatattcat agtttccgct cccatcggtg
      181 atctggtacc cgatgtagcg gtagtagccg gccttgagaa agaaatacgt cgtggtgagc
      241 tcaagctcgg taaagggctt ccagtaggta taggtgggcg cgccgcctga attatggtat
      301 cccttgcctc cgagggtcag gtggaacagg tcgtcctttt tctttccggc gtcggtgccg
      361 ttgtccgcct cgccgtcggc ttcttcatca tcggcggcat cgtcatcatc gtcggttatg
      421 ttcttcgtgg tgtcggtcct gccgccgttt cggtcttgcg ccatcagggc ggtccccatc
      481 gacgcaaacg ccagcaggca tcccagcaga gcggcgaaca ctttctttat cggtatctcc
      541 attgagggtt ccttgtcctt cgctgaatac atgatccggc tttcggtccg aacggcgcat
      601 cgggtaaact ttttctcgtc agcatcccgc tcgtcaatcc aataaacaag cccatggggc
      661 ggaaggtgca ctcccgctcc atgggcagcg aatcacggag aataatgtga tgcttacttg
      721 cctggttttt cacgctgctc cttgcgcagc tctttgcgct ccttgcgcag ctctttacgc
      781 tccttgcgga gttccttccg ttcgcggcgg agttcacgtc tggcctctcg gatttccttc
      841 ttgtccttag tgatggcagc cttgtcctgc ctgatggtct cttttagctg gcgcttcttc
      901 tcggggtcct tggtctgtcc aaggagcttc agatcggcct tgagcttggc ttggtcggtc
      961 tttagctgct cttgctttgc cttcaggctc tcctcggcgg ccctgacctc cttgcgctct
     1021 tcctttactt cccgtcgttc ctcacggact tctttccgct ccccggcgcc cggggtggtc
     1081 ccggtgtcct ggccataggc agccagcgtg accccaataa ccatcgcaat cgccatcagt
     1141 aacaatttct tcatgtgact cctccttgtg tgggttcatc gttgtcgaag actctgtctt
     1201 cagtgctcag tagtagtaca atcgagggcg tgcggacgga catttttttc agcaggggaa
     1261 ttaaaaaaag gtgcagcggg tcggtccctg cggatcacgg tgcggcaacc gatcccgtcc
     1321 ccacctgctc gtagatcctc ttctcgacga agagccggaa gagctccggg tcgatcttgt
     1381 tgtgcttcgc ctcgtcatcg aggatcgaaa gggcccgctc ggccgggagc gccttcttgt
     1441 aggggcggtc ggaggcggtg agcgcgtcgt agatatcggc gatcgccatc atcttggcct
     1501 ggatggggat gtcgccggcg gtgagtcggt tgggatagcc ggagccgtcc agcttctcgt
     1561 ggtgcttgag cgcgatctcc ggaatgcgtt tgaactccgg cggccacggg atcttgctga
     1621 cgaagttgta ggtgtggacc acatggctct cgatctcgcg gcgttcggat tcgttgaggc
     1681 tgccgcgggg aattcccagg ttcgtcttct cgtcgtcggt gagcacgcgc acgatattgc
     1741 cgtcgatgtc gtagcagcga aagccgcgca ccgtctcgtc gatctcgcat atccggcgct
     1801 cgatgtccgt gacggtgacc gcgggttcgt tgagccggcg cacctcctcg atgatgacct
     1861 tgatcgccgc gagcgtgcgt tcgcgctcct ccttccgtgt gcgcgcctcc tcgatgaggg
     1921 ccgggtccgc ccccctgacg gcgatctgcg cctcctccag cgagtagttg agctcgatgg
     1981 agcggtacag gtagttcatc ttgaggatga ggttctcgaa ctccttgggg aagagctttt
     2041 tccccttcat gaagatggcg atgtcgatgt agaccttgcc gaaatcgtgc aggagcgccg
     2101 cgtactcgag ctccttgacg gcgttctcgt tgaagttcac gtgcttgaac ggcccgtcgg
     2161 tcgcctgatt cacggcgtgc gcgagccggg tgcagatctc cgccacgcgg aacgagtggc
     2221 cggaggtggc cacgtcgcgc gattcgatcg ccgtcaccga cgcgcgcacg aactcctcga
     2281 actggttctc gatctggcgg atcatgcggt tgttctcgat ggcgatggcc gcctggctcg
     2341 cgaccgcctc catgaggctc tcgtagcggt gatcgaaggg caccaccttg gtctcgaagt
     2401 cctcgagcgt cgtcagccgc acctcgaagg cctcgttgcc ggtgaccgac gcgctgtcca
     2461 ggttctcctt gctgttgatg agctggatga ctccgatgat ctcgtcgatg tggttgcgca
     2521 tgggcacgac cagcatggac ttggagcggt agctctgcgt gcggtcgaag ttcgaattga
     2581 aggtgatggg gtcctcggtc gagagctcgt acacgtcggg gatgttgagc accgtcccgg
     2641 tgatggcgac gtagccggcg atggaattcg tgtcgtaggg tatcacgaat tcctccatgg
     2701 ggatctcctt cgagaaggtg tgcgaatact tgaagcgcag ctgcgtgccg ccgccggtcg
     2761 cgtcctcgac gaggtagatg ctgcccgcgt cggcgccggt gatccgcttg cttaaaaaca
     2821 ggatcgagcg caacagcttg tccgggtcct tctccagcga cagcgacttc ccgatgctga
     2881 tgaggtcctc ctggtcgtgc ttggcgtcca tcaggcggat ctggtagctc tggcgctcgg
     2941 ccgcggcggc gatctcgtcg accatcgcgt cgaaggactt ccgtaccagg aagatgaatt
     3001 cgcggtccat gatgggcgtg gtgcgcagct cgtggatata cttgagccgg cccatgttgg
     3061 acagcgtgtg gcggttctcc gggtccatcg ccacgatgcg gtaggcgagg tcgctgggct
     3121 tgaagctctt gaggagcgcg taggcctgtt cgtagtcgtc gatgtcgatg aagatgaccg
     3181 cgaacagcgt cccgcccttc ttcgatgcca ggatcttcgt gatctcgtcg atgatgcggt
     3241 cgctcaccac gacgctatcg gcatgttcgt cgggcacggc gatcaggtgg tcgatcgtcg
     3301 tcgagatgaa tatcctctgc agggaggaca gggacgttcc ggcgctcatg tcgggttcct
     3361 ccgcatgcat cacgctgccc cggggcgggg aataaatcaa ccactttatt gggcgcaccc
     3421 tcgcggcgcg ggatgatggc gggggcggga ataattgtac ttgacggatg aaaattttcg
     3481 ccgcaggctg caggcagcat tcatcatcct gcgccaactc ggagtcaagc accgtcatga
     3541 aacagtcaac gcgattcatc agcctcgcca ccgcctgcgt cgtcatcctc gccgcgtccg
     3601 tgagcgcgtc gtgcgccgcg gagaaaaaaa aggagaagcg cgcgcggccg cgcaccctcg
     3661 tcgagctcaa ccgggacctc atcgagctca cgcggcggac gcttccgtcg gtcgtgaaca
     3721 tctcggcggt caagaacatt gccgccgacg cgccgatgat gcggtttcgc attcccgacc
     3781 agaagcagcc cgatgcgccc cgcaagcgct cgtcgatcgg gtccggcgtc atcatcgacg
     3841 cgcgcggcta catcatcacc aacaaccacg tgatcaagaa cacgaagggc atcgaggtga
     3901 ccctttccga caagcgcgtg ttcaagtgca cggtcgtcgg ggccgatccc gcgaccgaca
     3961 tcgcggtgat ccgcatcgcg gacccggcgc cgaaggacct gcccgtcgcc gtcatcggcg
     4021 actccgacgc catccgcgtg ggcgagatcg tgatcgcgat cggcaatccc ttcggcttcg
     4081 cgcagaccgt gaccatgggc atcgtgagcg ccaccgggcg gcagaacatc gggctggcca
     4141 gctacgagaa cttcatccag accgacgccg ccatcaaccc cggaaactcc ggcggtgcgc
     4201 tggtgaacgt ggacggagtg ctggtgggga tcaacaccgc gatcttctcg cgcagcggcg
     4261 gctccatcgg catcggcttc gcgatcccgg ccaagatggt caagcaggtg atgagcgagc
     4321 tcatctcgtc cgggcgcgtt gtgcgcggct ggctcggggt gtacatccag gacgtcacga
     4381 aggacatcgc cgattccatc gggttcgccc gcaagggcgg cgcgatggtg tcggacatca
     4441 tgaagggatc gcccgccgag gggagcggcg tcgcggtggg cgacatcatc gtgtcggtca
     4501 acggcggcga gatccgggac gtcgatcagc ttcggaagct catcgccgcg ctcaagcccg
     4561 attcggtgat tacgctgggc gtgtttcgcg gcgggaagac gagcgaggtg aagatcaaga
     4621 tcggcgccat gcccgacgag ccccagcgtg ccgacacgcg aaagaccgag gccgacgacg
     4681 agctcggcat ggcggtgcgc gacgtcgatg aggcgctggc ctacaagtac aaggtcgccg
     4741 acggccgcgg ggtggtgatc gtcgccgtga aggcgggctc ccccgccggc aaggcgggac
     4801 tcatcgaggg cgacctcatc aaggaggtgg accagcggcc ggtgggaacc gcggcggcgt
     4861 atcggtccgt gctggcgacg ctcaagacgc gcacgaaggt gctcctcctc gtgaagcgcc
     4921 agggcatgaa caagttcgtg gtgatcaatc gggggccgga ggagtagccc ccgccgggtg
     4981 cgcatgggac acgccgagga aaaataccga cgcatcatcg aatcgctgcg cgagggctac
     5041 tacgaggtgg ccctcgacgg caccttcacc ttcgtgaacg acgccgtgtg cggcttcctg
     5101 ggcaggtccc gcgccgacgt gctggggacg cgctatcggg accacctgcc ggccgacatc
     5161 gccggcggcg tcgacgcggt cctggagtcg gtgcgcgtct ccgagcggcg ccgcgacctg
     5221 gtgcgcatcc cgctgcgccg catcgacggt tcaacggtcc agacggagca ctcgattgac
     5281 gttgtgcgcg atgattcggg ggccgtggcc ggcttccggg gcgtggcgcg cgacatcacc
     5341 ggacgcctgc gggccgagct ggagctgcgg gccagcgagg agaaattccg ccagctcgtc
     5401 gagaacatcg aggacgtcat ctacatcgtg aatccggcgg gccgcatccg ctacatctcg
     5461 ccctcggggc tgcgccggat cggccactcg gaggaggatc tcgccgcgcg tccctttttc
     5521 gaattcatcc aggaggagca ccgcgcggcg gcggtggcgt tctatcgcga tcagctttcg
     5581 cgcaacgttt cgaagacgtt ttacgagctt cccattataa aaaaggacgg gagcgtcatc
     5641 tgggtggagc agagcgtgaa gctggtgccc acggagggcg gcggtacgga gttctattgc
     5701 atcgcgcgcg acatcaccga aaagaagaag gcggtggagg cgctgcgccg gagcgaggag
     5761 cgctatcgga acatcctcgg cagcatcaac gacggctact acgaggtgga cctcgccggc
     5821 gcgatcacct tctgcaacga ggcgatggcg cgcacgctgg gctatccgcc ggaggagctc
     5881 gtctccatgg gctacgggca tttcatggac gagtcggaga tcaatatcgt ctattcgatc
     5941 ttcaacgagg tgtatcagac cggcgttccc tcggaggtgt tcgcgtacaa gttccgccgc
     6001 aaggacgggt cggtctgcat cgccgagacg acggcgtcgc tcgtgttcga cgaggcgggg
     6061 aggaaggccg gctttcgggg cattctgcgc gacatcacgg agcggaagaa cgccgaggac
     6121 gagcttcggc gcagcgagga aaagtaccga ctcatcatcg agcacgcgag cgacatcatt
     6181 tgcatgtgcg accggtccgg gcgcttcctg ttcatgaacc ccgcgggcct ggcgcggttc
     6241 gggtacaccg ccgagggtct catgggaatc cgctaccggg agctgatccc gacggcgcac
     6301 cgtgagcgcg tgcttacgtt ctaccgcgat cagctgcgca aacagatccg ccagacctac
     6361 ctcgagttcg aggcggcgac gcgtgacgga gagctcttct gggtggggca aaccgtgagc
     6421 atggtcgcgg acgccgacgg ggcgcttgag ttccacatcg tcgcgcgcga catcaccgcg
     6481 aaaaagcagg cggaggcggc gctcatcgag ctcgaccggc agaagtcgaa ttttttcgcg
     6541 aacgtgtccc acgagatccg gacgccgctc acgctgatcc tctcgccggt cgagtcggcg
     6601 ttgcagggcg atttcggcga ccgcgcggac cgcgattttc ttctcggcat acagcgcaac
     6661 gccatccggc tgctcaagct catcaacaat atcctcgatt tctcgaagat cgaggcgggg
     6721 cgcatgacga tgcggttgcg cgaaacggat atcgtcgcct tcctgcggcg ctatctgggc
     6781 agcatcgagt ctgcggcgga agcgcggaac atcgccctgg acctcgccgc ggcgccggat
     6841 gcgctcacgc tgctggcgga caccgagaag atggacaagg tcttcatgaa cctgttctcg
     6901 aacgccctca aattcaccgc gggcggcggg cgcatcacgg tgcgcgtgcg cgtcgacgcg
     6961 ggcgcctgcg tgatcgagat ggaggacgac ggcgagggga tccccgccga caagacgcgg
     7021 atcatctttg accgcttcgg acaggccgac accggaacca cccggcgcta cgaggggacc
     7081 gggatcgggc tggcgctggc aaaggagttc gtggagatgc acgacgggac catctcggtg
     7141 gtgagccggc acgtcgatgc gcacccggac gaccacggga gcgtcttcat catcaccctc
     7201 ccgatggacc gcgagcgctg ggcggggcgg cccggggtgg agatcgtcac gggctccgag
     7261 gtcgaggacg ccgtggcgga ccaccgcttc gcggggatgc gcgagatgac cgacctcgcg
     7321 gcgccggcgc cgcccgtgac cgcatcgacg cactcgccgg tcccgtccga ccggccgcgc
     7381 gtcctggttg tcgacgacaa cgccgacatg cgcgagttta tcgccgcgct cctggcgcgc
     7441 gactacgagg tgcacctggc cgtacacggg gccgacgggc tcgcgcgcgc cgacgaattg
     7501 cgcccggacc tgatcgtcac cgacgtgatg atgccggtga tgaacggcta cgagctcacg
     7561 cggcggctca aggcgcgcga cgatctgcgc gccctcccgg tcatcatgct caccgcgcgc
     7621 gcggagctgg ccaacaagat cgaggggctg gagttcggcg cggacgatta tctcaccaag
     7681 ccgttcaatt ccaaggagct gctcacgcgc gttgcggcgc tcctgaagag ctcgcgcttc
     7741 cagcgcatgc tggcggagcg caaccggatc atcgagaacg accttgcggt cgcgcggctg
     7801 attatcgacc gcatcctgcc gcggggcgtg ccggggacgg cggggttccg cttccacgcg
     7861 acctatctgc cggcggacac ggtgggcggc gacttttacg acatcgcggc gcgcgacggg
     7921 cggatcgagc tgttcatcgc cgacgtgtcg gggcacggtc tctcgagcgc cttcctggcg
     7981 atgatcgcga agatcacgct ggacgccatg cccgaccgct cgtcgcccgg gcgcgtcctg
     8041 gccgacctca acgaggcgat ttgccgcgcg acggtgcagg cgaacttcat caccgcgttc
     8101 tacggggtgg ttgaccgcgc cgatggcgtt atgcgctact gttcggcggg gcacgccccc
     8161 gccctcctgt tccggcgcgg gcgggacggg tgcgcggagc tgaacgccca ggggaagccg
     8221 atggggtggt tcgccggcga ggcgtacgag gagcgtcgtg agcggctcga gccgggggac
     8281 cgggtggtgc tgtacaccga cggcatcacc gaggcctggc gcgccgatac ggtgtgcgac
     8341 tgccgcgacc cggagtgcga catgttcggt caggagcggc tggcggcgtg cctgcgcgac
     8401 tgcgcggacc ggggaccgga gggcttctcg cgggagctca tggcgcgcct gcgcgcgttc
     8461 acggctacgg aacggttttc ggacgatatc acgatgatcg tgttcgacgt gacggcgccg
     8521 taggcatatc caggccgcac ccgcgccgcg tcagtagttc aatccgacct ttttcctggc
     8581 ctcgcgcagc gtgacggacg ccacggcgcg cgtcttctcg gcgcccgcgg cgagtatgcc
     8641 gcgcaccgcg ccggggtctt tcgccagttc ggcgcggcgc tcccggtagg ggcggaaaaa
     8701 ctcccagatg agctccacga gctccttctt gacctcgccg tagccggtcc cccctttcgc
     8761 ataacggtcg cgcaggttct ccacgcggtc cgccggggcg aaaaggcggt agaggttgaa
     8821 gacgttgcag gtctcggggt tcttcggcga ttcgacgggc gtcgagtcgg tgacgatctt
     8881 catcaccttc tttttgagc
//
LOCUS       DAORHF010000154         8859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7342_length_8859_cov_5.377101, whole genome shotgun sequence.
ACCESSION   DAORHF010000154 DAORHF010000000
VERSION     DAORHF010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8859
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7342_length_8859_cov_5.377101"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PKJ16_12485"
     CDS             complement(<1..369)
                     /locus_tag="PKJ16_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HNV47851.1"
                     /translation="MEPKLKGLKVLDFTYLLPGPYGTMMLADMGADIIKVENRANPDL
                     MRAVPPFVDGVSAAYAHLNRGKRSLALDLKTPESREIVIRLVREYDIVVEQFRPGVMD
                     RLGLGYDALRAANPRIIYCSL"
     gene            563..2716
                     /locus_tag="PKJ16_12490"
     CDS             563..2716
                     /locus_tag="PKJ16_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47852.1"
                     /translation="MELDKLVRQFIKYGAAVIGFNAFVLYAILSVAFPVFHDPRLIVL
                     FLVMGLTGFFGLFFYYLFRIKGLVRNTLAFLSQKEGFDRKRVAADYRGYARLTFLLNI
                     VMLCYMFIPSSVVIYFVFGYSNGYYLFYVLFVIVFLFLFLGYNSLTVWYTRTYPMGRF
                     GIPVQVQDLRSKIVSIVLPTVLMASVLIAVVVYNVNRAYMADIVYKRVFENLAHVAAH
                     LQEDGAVTDGLVPAIIPELDGAMILIDASGAVAFASTKDIAPGKPMREQIVQGRQAGY
                     LYERTRSVLEAGTGKLPPSFEGVMNGRQALFFSRVLPGGNRALLFVFPEQALLQNVYS
                     NIFLITVALFIINALIWLIVYTRMARTSRAVDAVMPAVFKATRGDLTQTFSVVKSRDI
                     LEDFTRSFATFVENVKSFMSQSVDLSRKLLELSEPIAEMGEHIKNSSTDHAALLVTST
                     HLVNAISASFGNIADRAQTQSTNIGDLEVLIARLFESMNRVTQDTGNIVKTMGGAEES
                     AHDGAQLIEIMTEGMRNTEELYSGILNVIQLISDIADQVNLLSLNASIEAARAGEYGR
                     GFSVVAEEISKLAENTGANVKEITSLINKGSVEIKRNMDNVLNVKQSFATIVTNVETA
                     GVMISGFLDMINKRIEEMAIVKDDITLISSYAKEISQATGEQRENTTQVSGTIEKVNV
                     GAKDFVDKSQRLSQMAKEMEGMAETLIENLKKFRL"
     gene            complement(2726..3679)
                     /locus_tag="PKJ16_12495"
     CDS             complement(2726..3679)
                     /locus_tag="PKJ16_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HNV47853.1"
                     /translation="MRAARITAPRTYTITDEPVPKPGKDQVLVRLLKTALCGSDLPYF
                     ANDFPAASYPLPFGYPGHECCGTVEESSHQSFKPGMRVMYYPTDLDAFREYHAVDAAR
                     LQRLPSEGDLNVLLMTQLLGAVAHCAFRIDSPYNKTVAIMGQGPVGLLFTALMRLLGA
                     KRIIAIDPLDFRRAAGIIMGADMTLDPRADDVPTAVREATGGTLADIVIDAYGQESAV
                     INACFELARHNGQVAFFGICLDEAPGLRFGTFFRKELRMISSVGPDLSMDYPYALDMI
                     TRGAVDVRPLITHVMPFERIQDAFDIAVGRRENAIKIILDF"
     gene            complement(3679..4974)
                     /gene="purB"
                     /locus_tag="PKJ16_12500"
     CDS             complement(3679..4974)
                     /gene="purB"
                     /locus_tag="PKJ16_12500"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HNV47854.1"
                     /translation="MIDRYARKEIADIWTLENKFRIWLDIEIAACEAHAQLGVIPAAA
                     LAIIKDKASFTVERITEIENQVHHDVIAFLTCVGEHVGPESRYIHYGLTSSDIVDTAQ
                     SIQLRQSGQIIRAGLAELVRITRELAIAHKMTPCMGRSHGVHAEPTTFGMKMALYHAE
                     FLRDDERLARAIEGISFGKLSGAVGTFSNIAPEVETMVCDILGLKPDPISTQVIQRDR
                     HAEFMAAIAITAGTLDQLATEIRHLQRTEVREVEEPFQKGQKGSSAMPHKRNPILCER
                     ITGLSRVIKSNLMVALENMTLWHERDISHSSAERVILPDSTVLLDYLIHRMIFILSNL
                     HIYPQNMLENIERTGGLFYSQTLLLALVEKGLSRETAYELVQAIAMRSWNREGSLKEL
                     ASKDSVIAKTLPGDELERVFDLTYFLRNIDHAFKKAGIQ"
     gene            complement(5073..5834)
                     /gene="recO"
                     /locus_tag="PKJ16_12505"
     CDS             complement(5073..5834)
                     /gene="recO"
                     /locus_tag="PKJ16_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HNV47855.1"
                     /translation="MRIHKTEGIVLAAQAHGEADRLLRVFTRDMGKRSFVLKGIRKSR
                     RRPQGAADPGTRLLLTFYEHDNRATHIVTELRIAAHCADIRDSMERICHLALMLETVD
                     GTSGFDDPNPTVFEMISAAVAALAATDSPVVLSLFFLLHLLRIHGVLPPMGACARCGK
                     PYADFAFGDADLRPVCAACARRDRPPPTLFPASARELLSTLLSTRFSAINTAALPVDT
                     ALDLLFRAVLFIEGYYHIEIKSKKLLFSLHRNDDA"
     gene            complement(5821..6675)
                     /locus_tag="PKJ16_12510"
     CDS             complement(5821..6675)
                     /locus_tag="PKJ16_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HNV47856.1"
                     /translation="MTSDTPDPDAQPNRIKRLLDRLRRDDARPPAAPDSPPQAGSEET
                     TQEEMIQGVISLSNKIAREIMIPRVDLIAIDATTSLKDLMKIIQDEGHSRYPVYESTV
                     DNVVGILHVKDLLKFILEKPKQFPMKKILRKPYFVPETMGLDDLLREFKKRRLHIAVV
                     VDEYGGVGGIITLEDILEEIVGDIDDEFDENEPPDVIKINQRTYEVDSRLTISDFIDE
                     TGFELPSDEFDTVGGLVLNLFGKIPAKNETASLGDLTFKIKDITGTRINRILVSRASK
                     KHAGDAHP"
     gene            complement(6845..7270)
                     /locus_tag="PKJ16_12515"
     CDS             complement(6845..7270)
                     /locus_tag="PKJ16_12515"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV47857.1"
                     /translation="MSKNRRQYPRIRLFRLTIKVKVKGADSFSDVELINLSAGGLCFI
                     RNSILNFGDALILRFEFKTRQVDMIGKIVRLDGREVGVQFTCTDDEIKEFVDSFNAEL
                     SSYLIPTASDGSRLELPGYTPRPQDKKSDIDAMLDIEPQ"
     gene            7397..8239
                     /locus_tag="PKJ16_12520"
     CDS             7397..8239
                     /locus_tag="PKJ16_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HNV47858.1"
                     /translation="MATQRTKTFFLPEPRVLAHRGYSAACPENTMPAFHKAVEFDPDV
                     LETDVRLTRDGRFVVFHDDTLERTTNGAGPVEQRTLDELRRFDAGFRFTRDNGATFPF
                     RGAGVFIPTLEDLLDAFPDRRFNIELKTKSPAQVESYLSILKRYDALDRVLTASEYGP
                     NIKELRRQCPTMATSFSLYEALWLYFLYKSGFLFKKKSFTADALQIPEYYGTSHVVTP
                     AFVRLMRERGVRVHVWTINRPEDMQRLLDMGVHGIFSDEVAILKNVVREHEARAADDG
                     PDGA"
     gene            8246..>8859
                     /locus_tag="PKJ16_12525"
     CDS             8246..>8859
                     /locus_tag="PKJ16_12525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47859.1"
                     /translation="MRIRLLALIFLLACSCGERVELLGVFGESRTADVLGQDGVTPIP
                     FDANTTLWTFGDTIIEAGKRGVSVEATFRERVADAHMLSNSLAFTAPLTEDTVRNPDF
                     VFYKEHGRIVQFIHYAPGEDPARIRLWALDGVRVGNRVYVYYVKIRIDDPEKLFAFTP
                     LAVGLARWNAPAGWRRGDPVRFQRLPDLFPGTHPAFGACVLERDG"
BASE COUNT         1648 a         2629 c         2781 g         1801 t
ORIGIN      
        1 gagcgaacag tagatgatgc gcgggtttgc ggcgcgcagc gcatcatacc ccagccccaa
       61 ccggtccatg acgcccggac ggaactgctc caccacaatg tcgtactcgc gcacgagacg
      121 gatgacgatc tcgcgcgatt cgggcgtctt cagatcgagc gcgagcgagc gcttcccgcg
      181 attgaggtgc gcataggccg ccgagacgcc gtccacgaag ggcgggaccg cgcgcatgag
      241 gtccggattg gcgcggttct ccaccttgat gatgtccgcg cccatatcgg ccagcatcat
      301 ggtgccgtag gggccgggga gcaggtaggt gaagtcgagg accttgaggc ctttgagttt
      361 cggttccatg agcgatacct agtgcgcggt atgccatttg gcaaatcaaa ttccgttgaa
      421 aatattgacc gacgctttaa tttttaattg aaattctgtt ttattcgttt atgctggttg
      481 ccggatgaga ttgagtgcca ggtggattgt ttcggcaaaa tagaaactgc cgtgacggtt
      541 taagatagaa aggggagtcc aaatggaact ggataagctc gtccgtcaat tcattaagta
      601 tggcgcggcg gtcatcggct tcaacgcctt tgtgctgtat gcaatcctgt ctgtcgcatt
      661 tccggtattt cacgatccac gtctcatcgt cctgtttctc gttatggggc ttaccggctt
      721 cttcgggctc tttttttatt acttgttccg catcaagggc ctggtgcgga acacgctcgc
      781 gtttttgtcg cagaaggagg ggttcgatcg aaagcgcgtc gccgccgatt atcgtggcta
      841 cgcgcgcctc accttcctgc tcaatatcgt catgctctgc tatatgttca ttccgagctc
      901 ggtggtcatc tatttcgtct tcggctattc gaacgggtat tatctcttct acgtgctctt
      961 cgtgatcgta ttcctgtttc tgttcctcgg atacaattcg ctcaccgtgt ggtacacgcg
     1021 gacctacccg atgggacggt tcggcatacc ggtccaggtg caagatcttc gcagcaagat
     1081 cgtcagcatc gtccttccca cggtcctcat ggcgtcggtg ctcatcgccg tggtggtgta
     1141 caacgtgaac cgcgcctaca tggccgacat cgtttacaag cgcgtgttcg agaacctcgc
     1201 gcatgtggcc gcgcatctgc aagaggatgg cgcggtgacc gacggactcg tgcccgccat
     1261 tattccggag ctggatgggg cgatgatcct gatcgacgcg tccggcgcag tcgcattcgc
     1321 ttcgacgaag gatatcgcac ccgggaaacc gatgcgggag cagatcgtcc agggacgcca
     1381 ggcgggctac ctctacgagc ggacacgcag cgtgctcgaa gcggggacgg ggaaactgcc
     1441 gccgtcgttc gagggcgtca tgaacggacg gcaggcgttg ttcttctcgc gcgtgctgcc
     1501 gggcggaaac cgcgcgctcc tgttcgtatt tcccgagcag gcattgctgc aaaacgtgta
     1561 ttcgaacatt ttcctgatca ccgtcgcgct ctttatcatc aatgcgctca tctggctcat
     1621 cgtgtacacg cgcatggcca ggacctcgcg cgcggtggac gccgtgatgc ccgcggtgtt
     1681 caaggccacg cggggcgatc tcacgcaaac gttcagcgtg gtaaaatcgc gcgacatcct
     1741 cgaggacttt acgcgctcgt tcgcgacatt cgtggaaaac gtgaaatcgt tcatgtcgca
     1801 atcggtggac ctgtcacgca agcttctgga gctctccgag ccaatcgccg agatgggcga
     1861 gcacatcaag aactcctcca ccgaccacgc ggccctgctg gtcacctcca cgcacctggt
     1921 caacgccatc tcggcgtcct tcggcaacat cgccgatcgc gcgcagacgc agtcgacgaa
     1981 catcggcgat ctggaggtgc tcatcgcgcg cctcttcgaa tcgatgaacc gggtcacgca
     2041 ggacaccgga aacatcgtca aaaccatggg cggcgccgag gagagcgccc atgacggggc
     2101 gcagctcatc gagatcatga ccgaggggat gcggaacact gaggagctct attccggcat
     2161 cctcaacgtg atccagctca tctccgacat cgccgaccag gtgaacctgc tatcgctcaa
     2221 cgcctccatc gaggccgctc gcgccggcga gtacggccgc ggcttctcgg tggtggccga
     2281 ggagatctcg aagctcgcgg agaacaccgg cgccaacgtg aaggagatca ccagcctcat
     2341 caacaagggg agcgtagaga tcaagcggaa catggacaat gtcctcaacg tgaagcagtc
     2401 attcgcgacg atcgtcacca atgtggaaac ggccggggtg atgatttccg gctttctgga
     2461 catgatcaac aagcgcatcg aggagatggc catcgtcaaa gacgacatca cgctcatcag
     2521 cagttacgcc aaggagatct cgcaggccac cggggaacag cgcgagaaca ccacccaggt
     2581 ttcggggacc atcgagaagg tgaacgtggg cgcgaaggac ttcgtggaca agtcgcagcg
     2641 gctctcgcag atggccaagg agatggaggg gatggccgag accctcatcg agaacctgaa
     2701 gaagttccgg ctgtaagttc gatcgtcaga aatcgaggat gatcttgatg gcgttttccc
     2761 ggcgccccac ggcgatgtcg aaggcgtcct ggatgcgctc gaacggcatc acgtgggtga
     2821 tgagcggacg cacgtccacc gccccgcgcg tgatcatgtc gagcgcgtag gggtagtcca
     2881 tcgagaggtc cggccccacc gaggatatca tccgcagctc cttccggaaa aacgtcccga
     2941 acctgagccc cggcgcctcg tcaaggcaga tgccgaagaa ggcgacctgc ccgttgtgcc
     3001 gcgcgagctc gaagcaggcg ttgatgaccg cgctctcctg cccgtaggcg tcgatgacga
     3061 tatcggcgag cgtccccccc gtcgcctcgc gcaccgcggt cgggacatcg tcggcgcgcg
     3121 ggtccagcgt catgtccgcg cccatgatga tgcccgccgc ccggcggaaa tccagcggat
     3181 cgatcgcgat gatccgcttc gcgcccagga gccgcatgag cgcggtgaac agcaggccca
     3241 ccggcccctg tcccatgatg gccacggtct tgttgtaggg gctgtcgatg cgaaaggcgc
     3301 agtgcgccac cgcgcccagc aactgcgtca tgagcaggac gttgaggtcc ccctcggatg
     3361 gaagccgctg caagcgcgcg gcgtccacgg cgtgatactc cctaaaggcg tcaaggtcgg
     3421 tcgggtagta catcacgcgc atgcccggct tgaaggactg gtggctcgat tcctcgaccg
     3481 tcccgcaaca ctcatgtccg ggatacccga acggcagcgg gtagctcgcc gccgggaaat
     3541 cgttcgcgaa gtagggcagg tccgatccgc acagcgccgt tttcaagagc cgaaccagca
     3601 cctggtcctt gcccggtttc gggaccggtt cgtccgttat cgtatacgtg cgcggcgccg
     3661 taatgcgtgc agctctcatt attgtatacc tgcctttttg aacgcatggt cgatgttgcg
     3721 aagaaagtag gtgaggtcga acacgcgctc gagttcgtcg cccgggagcg ttttcgcgat
     3781 aacggaatcc ttcgacgcaa gctccttcag gcttccctcg cggttccagc ttcgcatcgc
     3841 gatcgcttgc acgagctcat aggccgtctc gcgcgagagg cccttctcga ccagcgcgag
     3901 cagcagcgtt tgggagtaga agagcccgcc cgtccgctcg atgttctcca gcatgttctg
     3961 cggatagata tgcaggttcg acaggatgaa gatcatgcga tgaatgaggt agtccaaaag
     4021 caccgtcgag tcggggagga tcacccgctc ggccgatgag tgcgagatgt cgcgctcgtg
     4081 ccacagggtc atgttctcaa gcgccaccat caggttcgac ttgatcacgc gcgaaaggcc
     4141 ggtgatgcgc tcgcacagga tggggttgcg cttgtgcggc atggccgacg atcccttctg
     4201 gcccttctgg aagggctcct cgacctcgcg gacctcggtg cgctgcaggt gccgtatctc
     4261 cgtggcgagc tggtccagcg ttccggcggt gatggcgatc gccgccatga actcggcgtg
     4321 gcggtcgcgc tggatcactt gggtggagat ggggtccggc ttcaggccca gtatgtcgca
     4381 caccatcgtc tcgacctccg gcgcgatatt gctgaaggtc cccaccgcgc cggagagctt
     4441 cccgaaggaa atgccctcga tcgcgcgggc gaggcgctcg tcgtcgcgga gaaattcggc
     4501 gtgatagagc gccatcttca tcccgaaggt cgtaggctcg gcgtggaccc cgtgcgagcg
     4561 ccccatgcag ggggtcatct tgtgggcgat cgcgagctcc cgggtgatgc ggacgagctc
     4621 cgcaagcccc gcgcggatga tctggccgga ttgccggagt tgaatggact gcgccgtatc
     4681 gacgatgtcg ctggaggtga ggccgtagtg aatgtagcgg ctctccgggc cgacgtgctc
     4741 ccccacgcag gtgaggaagg cgatcacgtc gtggtggacc tggttttcga tctcggtgat
     4801 gcgctcaacg gtgaacgacg ccttgtcctt gatgatcgcg agcgccgccg cgggaattac
     4861 gccgagctgc gcatgcgcct cgcaggccgc aatctcgatg tcgagccaaa tccgaaactt
     4921 gttttccagc gtccagatgt ccgcgatctc ttttcgcgcg tatcggtcaa tcatggtgcc
     4981 ccctgtgtag agacagggca ttgccctgtc tctacacagg gggcggacgg gtggcgttca
     5041 aatcaacctt tttttaacgg ggccgatcct ttttaagcgt cgtcgttgcg atgcaacgaa
     5101 aagaggagct ttttcgattt tatttcgata tgatagtatc cttcgatgaa caggaccgcg
     5161 cggaacagga ggtccagcgc tgtgtcgacc gggagcgccg ccgtgttgat ggcggagaag
     5221 cgcgtcgaaa ggagcgtcga gagcaactcg cgcgccgatg cgggaaacag cgtcggcggc
     5281 gggcgatcgc gccgggcgca tgcggcgcag accgggcgca ggtcggcatc gccgaaggcg
     5341 aaatcggcat agggcttgcc gcatcgcgcg catgcgccca tcggcggaag cactccgtgg
     5401 atgcgcagca gatgcagcag gaaaaagagc gacaggacca ccggcgagtc ggtggcggcg
     5461 agcgccgcga cagccgcaga gatcatctcg aaaacggtgg ggttcgggtc gtcgaacccg
     5521 ctcgtgccgt ccaccgtctc gagcatgagc gcgaggtggc agatccgctc catgctatcg
     5581 cgaatgtcgg cgcagtgcgc ggcgatgcgg agctccgtga cgatgtgcgt ggcgcggttg
     5641 tcgtgctcgt aaaaggtcag gaggaggcgg gttccgggat cggccgctcc ctgcggtcgt
     5701 cgtcggctct tgcgaatgcc cttgaggacg aaggaacgtt tgcccatgtc gcgggtgaac
     5761 acgcgtagca ggcggtcggc ctcgccgtgc gcctgggcgg cgaggacgat cccctcggtc
     5821 ttatggatgc gcatcgcccg cgtgtttttt cgaggcgcgg ctcaccagga tgcggttgat
     5881 gcgggttccc gtgatgtcct tgattttgaa ggtgaggtcg ccgagcgaag cggtctcgtt
     5941 cttggccggg atctttccga agagattgag gacgagcccg cccacggtgt cgaattcgtc
     6001 cgacggcagc tcgaagcccg tctcgtcgat gaagtcggat atcgtgagcc gcgagtcgac
     6061 ctcgtaggtt cgctggttga tcttgatgac gtccggcggc tcgttctcgt cgaactcgtc
     6121 gtcgatgtcg cccacgatct cctcgaggat gtcctccagg gtgatgatgc cgcccacgcc
     6181 gccgtattcg tccaccacca ccgcgatgtg caggcggcgt ttcttgaatt cgcgcaacag
     6241 gtcgtcgagc cccatcgtct cggggacgaa gtacggcttg cggaggatct ttttcatggg
     6301 gaactgcttg ggcttctcga ggatgaactt aagcaggtct ttcacatgaa ggatgcccac
     6361 cacgttgtcc acggtgcttt cgtacacggg atagcgcgaa tgcccctcgt cctggatgat
     6421 cttcatgagg tccttgagcg aggtggtcgc gtcgatcgcg atgaggtcca cgcgggggat
     6481 catgatctcg cgcgcgatct tgttcgagag gctgatgacg ccctggatca tctcctcctg
     6541 cgtggtctcc tcggaaccgg cctgcggcgg cgaatccggg gccgcaggcg ggcgcgcatc
     6601 atcccgacgg aggcgatcga gaaggcgttt gattcggttt ggttgtgcgt ccgggtcggg
     6661 agtgtcagat gtcatcaata tcctggcgcg tgtgcgtatc agatcgaaat ataattgata
     6721 atgcaacgcc gcggggcgtc attgttggga ttttgtaaag atcacccgcg gtcgtccggt
     6781 ccgatatcaa cccgtagcat gccccggtga gaaatcaatc aaaaaaatga ggggcgcgtc
     6841 gttttcactg cggctcgatg tcgagcatgg cgtcgatgtc gctcttcttg tcctgcggcc
     6901 gcggcgtgta accgggaagt tccaggcggg agccgtcgct ggcggtaggg atgagatagc
     6961 tcgaaagctc ggcgttgaac gaatcgacga actccttgat ctcgtcgtcg gtgcatgtga
     7021 attgcacgcc gacctcgcgg ccgtcgaggc ggacgatctt cccgatcatg tccacctggc
     7081 gcgtcttgaa ctcaaagcgc aggatgagcg cgtccccaaa attaagaatg ctgttgcgga
     7141 taaagcacaa tccgccggcg gagaggttga taagctcgac gtcggaaaac gaatcggcgc
     7201 ccttgacctt caccttgatg gtgagccgaa aaagcctgat gcgcggatac tgccgtctgt
     7261 ttttactcat gtgcctccgg aagacgcttc gccggtgctt ttttcacttg atgcgcaacg
     7321 caccgcaatg catagtgtac gaaatcgctt gtttgcaatc aagtgatttt tccatttttc
     7381 caatgggggc atgcggatgg caacacagcg gaccaagacc tttttcctgc cggagccgcg
     7441 cgtgctcgcg caccgcggat attccgccgc ctgcccggag aacacgatgc cggcgtttca
     7501 caaggccgtc gagttcgacc cggacgtgct cgagaccgac gttcgcctca cccgggacgg
     7561 gcgcttcgtg gtgtttcacg acgatacgtt ggagcgcacc accaacggcg ccgggccggt
     7621 cgaacagcgc acgctcgacg agctccggcg attcgatgcg ggctttcggt tcacccggga
     7681 taacggcgcc acctttccgt tcaggggggc gggcgtcttc atcccaacgc tggaagatct
     7741 cctcgacgca ttcccggatc ggcggttcaa catcgagctc aagacgaagt cgcccgcgca
     7801 ggtggaatcg tacttgtcga tattgaagcg atacgacgcc ctcgaccgcg tgctcaccgc
     7861 atcggagtac gggcccaaca tcaaggagct tcggcgccag tgtccgacca tggcgacctc
     7921 gttttcgctc tacgaggcgc tctggctgta tttcctctac aagtcgggct tcctctttaa
     7981 aaagaaatcc tttaccgccg acgcgctgca aatcccggaa tattacggta cgtcgcatgt
     8041 cgtcacgccg gccttcgtgc ggttgatgcg cgaacgcggt gtccgcgtcc acgtgtggac
     8101 cataaaccgc cccgaggaca tgcagcggct tctcgacatg ggcgttcacg gcattttttc
     8161 cgacgaggtc gcgatcctga aaaatgtcgt ccgtgaacac gaggcgcgcg ccgccgacga
     8221 cgggccggac ggggcatagg cgccgatgcg catccggctc ctcgcgctga ttttcctact
     8281 cgcctgctca tgcggggagc gcgtcgaact cctcggcgtc ttcggcgaat cgcgcacggc
     8341 ggatgtcctg ggacaggacg gcgtgacgcc cattcccttc gatgcaaaca ccacgctatg
     8401 gacattcggc gacaccatca tcgaagcggg aaagcgggga gtgtcggtcg aggcgacatt
     8461 tcgcgagcgc gtggcggacg cgcacatgct ttcgaactcg ctggccttca ccgcgccgct
     8521 caccgaggac accgttcgca atccggactt cgttttttac aaggaacacg gtagaatcgt
     8581 ccaattcatt cactatgccc cgggcgagga cccggcgcgc atacgcctct gggcgctcga
     8641 cggcgtcagg gtcggcaatc gcgtctatgt ctattatgtg aagatacgga tcgacgatcc
     8701 ggagaagctc ttcgccttta cccccctcgc cgtcggtctc gcgcgctgga atgcgcccgc
     8761 cggatggcgc cgcggcgatc cggtgcgctt tcagcgactg ccggacctgt ttcccggcac
     8821 gcacccggca ttcggcgcct gcgtgttgga gcgcgatgg
//
LOCUS       DAORHF010000155         8836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7373_length_8836_cov_5.466576, whole genome shotgun sequence.
ACCESSION   DAORHF010000155 DAORHF010000000
VERSION     DAORHF010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8836
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7373_length_8836_cov_5.466576"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PKJ16_12530"
     CDS             complement(<1..354)
                     /locus_tag="PKJ16_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV47860.1"
                     /translation="MKRFPEKRALITGAGSGLGRALALAFAKRGWRVAAADIDGARAE
                     ETRGAIDAAGGTGLAIRCDVTRRAEIEAARGIVQREFGGIDVLVNNAGIAAAGRIEDI
                     TPSEWDRIIAINLRGV"
     gene            complement(351..3602)
                     /locus_tag="PKJ16_12535"
     CDS             complement(351..3602)
                     /locus_tag="PKJ16_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011163537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV47861.1"
                     /translation="MTLSDISIKNPVFAWLLMVGLIVFGAIGFARMGISQLPDVDFPV
                     LTIMVTWSGAAPDTMETAVADVIENAVMSVEGVRTVSSVSQEGLTNITIEFELGRDIN
                     AALQEVQSNIYQAQRNLPKDIDPAIIRKSNPEDNPIMFTALTGEAPLREKMLYARDHL
                     RDALSTVEGVGDIRMGGFVEPNMRIWLDKTRMNAREIVVEDVIAAVNGQHGLSPSGYI
                     DTGPKEKNVRVLSEANTANEFKNLVIPNRRGQPIWIPIRLGDVADIEEGLADVRRISR
                     FNGERAAGLGIVKQRGSNAVAVAKAVKKRIANLADILPKTMKVGVIWDSTKFIEDATR
                     ELLFTLTMAVILTSVVCWLFLGSMSSAFNVILAIPTSLVGSFIALYFFGFTLNTFTLL
                     ALSLSIGIVVDDAIMVLENIVRHNEEGMSRMKAAIVGAREITGAATAASLAILAIFVP
                     VVFMKGIIGKFFFQFGVAMSVAVMLSLLEALTLAPMRCSQFLSSGSDTRIGRRVDAAM
                     GFLSERYSGALQWCLNHRVKVIGVSLAVFSVSLLLVVGMRKEFVPPQDQSRFMINIQT
                     PLGSSIGFTDDAFKKIEAILSERPEVAAYFCAVGGFQGGLVNQGIFNVTLKDPKERPL
                     APPFKSVPSQQQFMAFLRERIKAVPGVFRVSMIDLSLMGFSAQRGFPIQFSVQGPDWG
                     KLAEHSQRIMDAMNASGLVTDIDTDYKMGMPEVTVRPDRAKAAARGVTVGNIADTISA
                     SVGSLRIGKYTDAAGHRDDIRVKLLDKYNRDPADIRRLAVRNMQGELVPLADLVTLDE
                     NPSLLTITRYNRERAISIFANIAPGKSQSDIMAFIEKTAKDTLPAGYHAVFSGSSETF
                     KESFDSLILALVLGIFVAYIVLGSQFNSFIHPVTVLLALPFSVTGAFIALRLTGISLN
                     IYSMIGLLLLMGIVKKNSILLVDFTNKRRVDGLGVREALLAACPTRLRPIIMTSVATV
                     AAAIPAALAIGPGAETIRPMAVVVIGGVAFSTVLTLFVVPCAYSVFSRFESRRHQREL
                     HEALVSLGEVEAEAHARTETIDVSLSIDMPTTEAAPAERPSRGGKKPLSRKRT"
     gene            complement(3599..4981)
                     /locus_tag="PKJ16_12540"
     CDS             complement(3599..4981)
                     /locus_tag="PKJ16_12540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV47862.1"
                     /translation="MLPRTLITIALLAVSLSAAAMSADQSRLIRRASGVPEEDSAGAA
                     PLRSLGLYDLYALAVRNTERMKIEAENVEQAIARKNQAIGSFLPRVSLRAAKVFPEDA
                     ETLSSFATIRTGVYLIARQPIFTGLDEWTRYRGAVPDLRMQQFNARFAASRLLFDVSY
                     AYFRVLQIEKSLASRTEILRLYGRNIQELRRRVHLGKSRQSDVLRTQSQIHKIEADIL
                     AFTNELERARLDLSTITGIDIGKRELRETADPPRPLPVAENMRAYVEDRWDVRAAVES
                     VAYAKTRVLAAKGGHLPQVYVEGGYRFYQPEDIKKGPDYFVAIGADFPIFAGLTVVSK
                     TREAESAMRQAELRLRQARRIARQDIIDSNQSYETSTREIEAYRKALATADENYRTVL
                     RDYRLNLVSVLDVFSALTTLQSAQEDFDRVSLQHRLNRIRLGIAVNEFAAGGIETLRR
                     ISAPIGGTGR"
     gene            5259..8567
                     /locus_tag="PKJ16_12545"
     CDS             5259..8567
                     /locus_tag="PKJ16_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV47863.1"
                     /translation="MKRLIAYFVERSLLVNIFSVGVLIAGILFLVTAKREAFPRIEFD
                     YVIVNTVYPGATAEDVEKHISIPIEDRLREVDGIEELNSSSLEARSVVAVKLDPDLEN
                     KDKVINNIRNAVDLVKDFPADAEQPEVEELTSAMMPVLEIALFNDRGIANDADEFELR
                     KYAKILEDRLLDVPGVGRVDKKGYRDREMIVEVSPEKLDAYRVAINEVIMGLSKKNLN
                     FPGGVIKNPTGEVVIRTIGEVQSARDIENVFLRTSEVGTSVRVGDVATVRDSFEEEKI
                     LDRVKGRPAIVLTVIKKESADIITLVDKVRVELGRYKKILPEHYRTSEFNDSSYYVKR
                     RLDVLVSNGIVGFILVLGVLFLSLGWRISFVTALGIPFAFLATFIWMGWYGLSVNLMS
                     MFGLIMVLGMLVDDAIVVAENVYRHIEMGEPIKKAVIDGTAEVMTPVAGTVLTTIAAF
                     MPLMFMTGIMGKFMWSLPAVVVVALAASWVECMLILPSHINDIESRFRARTGHVAGEE
                     GRIFVSIRERYRRMLAFVLEHRYIAAVVIGALFVGSIAFAATQMKLILFPQGGVEVLV
                     VKAEAPGGTTVRDMSRRLGHIEQIIGALPAKELDTFTTRAGIIQENPSDPYTKRGSNY
                     GIIMVYLTPFQQRERQADRILDDIRKKSAAHEKEFVKLEFSMVKTGPPVGKPISVAIK
                     GEDFAEMQKMAAEFKRHLAGIAGLKDVKDDFEEVKDKLRVTVNEKLAAMTGITVYDIA
                     TTIRSSFEGTVATKIKKSNEEIDIRVIFPERLRKNPSSVRHIKVANRMGNLIPLSAVA
                     QFERDRGISVIKRKDWRRVITVTAEIDERAKNVSSVEVNRSLQKAFAGIEERHAGISV
                     NYEGEFKDTSDSVMRLFRSFLIAALVIYIILVAIFRSVTHPVIIMSVIPLSFIGVIWS
                     FFLHGHPFSFLGIMGVVGLTGVVVNDSIVMVDFIKVSRNRGLSPLAATLEAGATRLRP
                     IFLTTITTFFGLLPTAYGIGGYDPFLVPMALSLSWGLAFGSLITLFATPVLYNLFSDF
                     RQRLFHRDTELLRPEFPPSTIQEMESEIEQVVREDLKDDMRAAVRDEVRRQMEAERAE
                     RAGRSRRPNKGPRR"
     gene            8611..>8836
                     /locus_tag="PKJ16_12550"
     CDS             8611..>8836
                     /locus_tag="PKJ16_12550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV47864.1"
                     /translation="MKRQAVAPVPLRSATRQDCVACGQRCCRSISFEVDTPASPEDFD
                     HLRWMLAHHNVFVYIEHGEWYVEVAVDCAML"
BASE COUNT         1535 a         2753 c         2962 g         1586 t
ORIGIN      
        1 cacgccccgc aggttgatgg cgataatgcg gtcccattcc gacggcgtga tgtcctcgat
       61 ccggccggcg gcggcgatcc cggcgttgtt caccaacacg tcgatcccgc cgaactcgcg
      121 ttgcacgata ccccgcgccg cctcgatctc ggcgcgtcgc gtcacgtcgc agcggatcgc
      181 caggcccgtc ccgcccgccg cgtcgatcgc gccgcgcgtt tcctccgcgc gcgccccgtc
      241 gatgtcggcc gcggcgacgc gccagccgcg tttcgcgaag gcgagcgcga gggcgcgtcc
      301 gagcccgctt cccgcgccgg tgatgagcgc gcgtttttca gggaatcgtt tcatgtccgc
      361 ttcctcgaca atggtttttt cccgccgcgc gacgggcgct cggcgggtgc ggcctcggtc
      421 gtcggcatgt cgattgaaag ggagacgtcg atcgtctccg ttcgcgcgtg cgcctcggcc
      481 tcgacctcgc cgagggagac gagcgcctcg tggagctcgc gctggtgccg ccggctctcg
      541 aaccggctaa acacgctgta ggcgcacggg accacgaaga gcgtgagcac ggtggagaag
      601 gccacaccgc cgatcaccac aaccgccatg gggcggatcg tctccgcgcc ggggccgatg
      661 gcgagcgcgg ccggaatagc ggccgccacg gtggcaaccg aggtcatgat gatggggcgc
      721 aggcgcgtgg ggcaggccgc caggagagcc tcgcgcaccc cgagcccgtc gacgcggcgc
      781 ttgttggtga aatcgaccag aaggatcgag ttctttttca cgatgcccat gagcagcagg
      841 aggccaatca tactgtagat gttgagcgag atcccggtga gccggagcgc gatgaaggcc
      901 ccggtgacgc tgaaggggag cgccaagagg accgtgaccg ggtggatgaa gctgttgaac
      961 tgcgagccga ggacgatgta ggccacgaag atccccagca cgagcgcgag gatgaggctg
     1021 tcgaaggact ccttgaacgt ttccgagctt cccgagaaga ccgcgtgata gcccgcgggg
     1081 agggtgtcct tggcggtctt ctcgatgaac gccatgatgt cggactgcga tttcccgggg
     1141 gcgatgtttg cgaagatgga gatcgcgcgc tcgcggttgt agcgcgtgat cgtgagcagg
     1201 gacgggttct cgtcgagcgt caccaggtcg gcaagcggca ccagctcgcc ctgcatgttg
     1261 cgcacggcga ggcggcggat gtcggcggga tcgcggttgt acttgtccaa aagcttcacg
     1321 cggatatcgt cgcggtggcc ggccgcgtcg gtgtacttgc cgatgcgcag gcttcccacc
     1381 gacgcgctga tggtgtcggc gatattgccc acggtcacgc cgcgcgcggc ggccttcgcg
     1441 cggtccggcc gcaccgtgac ctcgggcatc cccatcttgt agtcggtgtc gatgtcggtt
     1501 acaagcccgg aggcgttcat tgcgtccatg atgcgctggc tgtgctcggc gagcttcccc
     1561 cagtcgggac cctgcaccga gaactggatg gggaaaccgc gctgggcgga gaagcccatg
     1621 agcgaaaggt cgatcatgct cacgcgaaag acgccgggga ccgctttgat ccgctcccgc
     1681 aggaaggcca tgaactgctg ctgtgagggg acggatttaa atggaggcgc gagcgggcgc
     1741 tccttggggt ctttgagggt gacattgaag atcccctggt tgacgagacc gccctggaaa
     1801 ccgcccaccg cgcaaaaata ggcggcgacc tcgggccgtt ccgaaaggat cgcttcgatc
     1861 ttcttgaacg catcgtcggt gaagccgatg gaggatccca gcggcgtctg gatattaatc
     1921 atgaaccggc tctggtcctg aggcgggacg aactccttgc gcatgccgac caccagcagg
     1981 agcgataccg agaagacggc gagcgagacg ccgatcacct tcacgcggtg gttgagacac
     2041 cactgcagcg cgccgctgta gcgctcggac aggaagccca tcgcggcgtc cacgcgtcgg
     2101 ccgatgcgcg tatcgctccc cgacgagagg aactgcgagc agcgcatggg cgcgagggtg
     2161 agcgcctcga gcagcgaaag catcaccgcg accgacatcg ccacgccgaa ctggaagaaa
     2221 aacttgccga tgattccctt catgaacacc accggcacga agatcgcgag gatcgcgagc
     2281 gaggccgccg tcgccgcgcc ggtgatctcg cgcgcgccca cgatcgccgc cttcatgcgc
     2341 gacatgccct cctcgttgtg ccgcacaatg ttctcgagca ccatgatcgc gtcgtccacc
     2401 acgatcccga tggacagcga cagcgccagc agcgtgaagg tgttgagcgt gaagccgaag
     2461 aagtacagcg cgatgaacga gccgacaagc gaggtgggga tcgccaggat cacgttgaac
     2521 gcggagctca tcgagcccag aaagagccag cacacgacgg acgtgaggat gaccgccatc
     2581 gtgagcgtga agaggagctc ccgggtcgcg tcctcgatga acttcgtgct gtcccagata
     2641 acgccgacct tcatggtctt gggcagaatg tcggcgaggt tggcgatgcg ttttttgacc
     2701 gccttggcga ccgccacggc gttggagccg cgctgtttca cgatgcccag gcccgccgcg
     2761 cgctcgccgt tgaagcgcga gatgcgccgg acgtcggcca ggccctcctc gatgtcggcc
     2821 acgtccccca ggcggatggg aatccagatg ggctgcccgc gccggttggg gatcaccagg
     2881 ttcttgaact cgttcgcggt attcgcctcg ctgagcaccc gcacgttctt ctctttgggc
     2941 ccggtgtcga tatacccgga gggcgagagg ccgtgctggc cgttcacggc ggcgatcacg
     3001 tcctcaacca cgatctcgcg cgcgttcatg cgggtcttgt cgagccagat gcgcatgttc
     3061 ggttccacga agccgcccat gcggatgtcg cccacgccct ccacggtgga cagcgcatcg
     3121 cgcaggtggt cgcgcgcgta gagcattttc tcgcgcagcg gggcctcgcc ggtgagcgcg
     3181 gtgaacatga tcgggttgtc ctcggggttc gatttcctga tgatggccgg gtcgatgtcc
     3241 ttggggagat tgcgctgcgc ctggtagatg ttggactgca cctcctgcag ggcggcgttg
     3301 atgtcgcggc ccagctcgaa ctcgatggtg atgttggtga gcccctcctg cgagaccgag
     3361 ctcacagtgc gcacgccctc gacgctcatc accgcgttct cgatcacgtc cgcgaccgcg
     3421 gtctccatgg tgtcgggggc ggccccggac caggtgacca tgatggtgag caccgggaag
     3481 tccacgtccg ggagctggct gatccccatg cgggcgaagc cgatcgcgcc gaagacgatg
     3541 agcccgacca tgagcagcca ggcgaagacg gggttcttga tcgatatgtc ggataacgtc
     3601 atcggcccgt ccccccgata ggcgccgaga tgcgccggag cgtttcgatc ccgccggcgg
     3661 cgaactcgtt cacggcgatg cccaggcgga tgcggttgag ccggtgctgg aggctcacgc
     3721 ggtcgaagtc ctcctgcgcg ctctgcaggg tggtgagcgc cgagaagacg tcgagcacgc
     3781 tcaccaggtt gagccggtag tcgcgcagca cggtgcgata gttttcgtcc gccgtcgcca
     3841 gcgcctttcg gtaggcctcg atctcgcgcg tggaggtctc gtagctctgg ttcgagtcaa
     3901 tgatgtcctg gcgggcgatg cggcgcgcct ggcggaggcg gagctcggcc tgccgcatcg
     3961 cggattcggc ctcgcgcgtt ttcgagacga ccgtgagccc ggcgaagatg ggaaagtccg
     4021 cgccgatcgc cacgaagtaa tcgggcccct tcttgatgtc ctcgggctgg tagaaccggt
     4081 agccgccctc gacgtacacc tgcggaaggt ggccgccctt ggccgcgagc acgcgcgttt
     4141 tcgcgtaggc cacgctctcg acggccgcgc gcacgtccca gcggtcctcc acgtatgcgc
     4201 gcatgttctc cgcgaccggc aggggccggg gcggatcggc cgtctcgcgg agctcccgtt
     4261 tcccgatgtc gatgccggtg atggtggaga ggtccaggcg ggcgcgctcg agctcgttgg
     4321 tgaaggcgag gatgtccgcc tcgatcttgt ggatctgcga ctgggtgcgc agcacgtcgc
     4381 tctggcggct cttgcccagg tgcacgcggc ggcggagctc ctggatgttg cgcccgtaca
     4441 gccgcaggat ctcggtgcgg ctggccaggc tcttctcgat ctgcagcacg cggaaatagg
     4501 cgtacgagac gtcgaagagc aaccgcgatg cggcgaagcg cgcattgaac tgctgcatgc
     4561 gcaggtcggg cacggcgccg cggtagcgcg tccattcgtc cagccccgtg aagatgggct
     4621 gccgcgcgat gagatacacg cccgtccgga tggtggcgaa cgagctcagc gtctcggcgt
     4681 cctcggggaa gaccttcgcc gcgcgcaggg acacccgcgg gagaaacgaa ccgatcgcct
     4741 ggttctttcg cgcgatcgcc tgctcgacgt tctcggcctc gatcttcatc cgttcggtgt
     4801 tgcgcaccgc gagcgcatag aggtcgtaga gccccagcga gcgcaacggc gcggcgccgg
     4861 cgctgtcctc ctcgggaacg ccggaggcgc gacggatcag ccggctctgg tcggcgctca
     4921 tggccgcagc ggagaggctt acggcgagca gcgcgatggt gatgagtgtc cgtgggagca
     4981 ttgtgattga attatagttc gacccggtcg atgggggcgt cggttcccgt tttttcgccg
     5041 aggagggcgt ttttcgcgct ttccgcgcgc cggtcgttcg gcaggccgta cgctttatca
     5101 tacatacgac gctggcaagc atatttttgc cgggtgcgcg ataccgcatc gcgggcgtgc
     5161 tgcgagaaaa tttacatcgc tggcgaacaa ttttctttac aaacgaagga cccgctgttc
     5221 aagtctgcgc cagaccgatt accgaacggg gcccacccat gaaacgactt atcgcctact
     5281 tcgtcgaacg ctcgctcctg gtgaacatct tctctgttgg cgtgcttatc gcgggcattt
     5341 tgttccttgt cactgcaaaa cgcgaggcct ttccgcgcat cgagttcgat tacgtgatcg
     5401 tgaacaccgt ctaccccggc gcaacggccg aggacgtcga gaagcatatc agcattccca
     5461 tcgaggaccg gcttcgggag gtggacggca tcgaggagct caattcgagc tcgcttgagg
     5521 cgcgttccgt cgtcgcggtg aaactcgatc ccgacctcga gaacaaggac aaggtgatca
     5581 acaatatccg caacgccgtg gacctggtga aggacttccc ggccgacgcg gagcagccgg
     5641 aggtggagga gctcacctcg gccatgatgc cggtgctcga gatcgcgctc tttaacgatc
     5701 ggggcatcgc gaacgacgcc gacgagttcg agctgcggaa atacgcgaag atcctcgagg
     5761 accgcctgct ggacgtgccg ggcgtggggc gcgtcgacaa gaagggctat cgcgaccgcg
     5821 agatgatcgt ggaggtgagc cccgagaaac tggacgccta ccgtgtcgcc atcaacgagg
     5881 tgatcatggg gttgtccaag aagaacctga acttccccgg gggcgtcatc aaaaatccca
     5941 cgggggaggt ggtgatccgc accatcggcg aggtgcagag cgcacgcgac atcgagaacg
     6001 tgttcctgcg caccagcgag gtgggcacca gcgtccgcgt gggcgacgtg gccacggtgc
     6061 gcgattcctt cgaggaggag aagatcctcg accgcgtgaa gggccggccc gcgatcgtcc
     6121 tgaccgtcat caagaaggaa tcggccgaca tcatcaccct ggtggacaag gtgagggtcg
     6181 agcttgggcg ctacaagaag atcctgccgg agcactaccg gacctctgag ttcaacgact
     6241 cgtcgtatta cgtgaagcga cggctcgacg tgcttgtttc gaacgggatc gtgggtttta
     6301 tcctggtttt gggcgttctg ttcctctccc tcggatggcg aatatcgttc gtgaccgcgc
     6361 tcggcatccc attcgccttc ctggcgacct ttatctggat ggggtggtac ggcctcagcg
     6421 tgaacctcat gtccatgttc ggcctcatca tggtgctggg gatgctggtc gatgatgcca
     6481 tcgtggtggc ggagaacgtg taccggcaca tcgagatggg cgagccgatc aagaaggcgg
     6541 tgatcgacgg gaccgccgag gtgatgacgc ccgtcgccgg gaccgtgctc accaccatcg
     6601 ccgccttcat gccgctcatg ttcatgaccg ggatcatggg gaagttcatg tggtcgctcc
     6661 ccgcggtggt ggtggtggcc ctggccgcct cgtgggtgga gtgcatgctt attctgccgt
     6721 cgcacatcaa cgacatcgag agccgtttcc gcgcccggac ggggcatgtt gccggcgaag
     6781 agggacgcat attcgtgtcg atccgggagc gctatcgccg catgctcgcg ttcgtgctcg
     6841 agcatcgcta tatcgccgcg gtcgtgatcg gcgcgctctt cgtcgggtcc attgctttcg
     6901 cggcgacgca gatgaagctc atcctcttcc cgcagggcgg cgtggaggtg ctcgtcgtga
     6961 aggcggaggc gcccggcggg accacggtgc gggacatgag ccggcgcctc ggccacatcg
     7021 aacagatcat cggcgcgctc ccggccaagg agctcgatac cttcaccacc cgcgcgggaa
     7081 tcatacagga aaacccgagc gatccctaca ccaagcgcgg atcgaactat ggcatcatca
     7141 tggtctacct cacgcccttt cagcagcgcg aacggcaggc cgaccgcatc ctggacgaca
     7201 ttcgaaaaaa atcggccgcg catgaaaagg agttcgtgaa gctcgagttt agcatggtca
     7261 agaccgggcc gcccgtgggc aagcccatca gcgtggccat caagggcgag gacttcgccg
     7321 agatgcagaa gatggccgcg gagttcaagc ggcacctcgc gggcatcgcg gggctcaagg
     7381 acgtcaagga cgatttcgag gaggtgaagg acaagctgcg cgtgaccgtg aacgaaaagc
     7441 tcgccgccat gaccgggatc acggtgtacg atatcgccac caccatccgc tcgagcttcg
     7501 aggggacggt cgcgaccaag atcaagaagt ccaacgagga gatcgatatc cgcgtgatct
     7561 tcccggagcg cctgcggaaa aacccctcga gcgtgcggca catcaaggtg gccaaccgga
     7621 tgggaaatct catcccgctc tcggcggtcg cgcagttcga gcgcgatcgc ggcatctccg
     7681 tcatcaagcg caaggactgg cggcgcgtca tcaccgtgac cgccgagatc gacgagcgcg
     7741 cgaagaacgt ctcgtcggtg gaggtgaacc gctcgctcca aaaggccttc gcgggcatcg
     7801 aggagcgcca cgcgggcatc tccgtgaact acgagggcga gttcaaggac acctccgatt
     7861 cggtcatgcg gctctttcgc tccttcctga tcgcggcgct ggtcatctat atcatcctgg
     7921 tggccatctt ccgttcggtc acgcatccgg tcatcatcat gagcgtgatc ccgctgtcgt
     7981 tcatcggcgt gatctggtcc tttttcttgc acggccatcc attttcgttc ctgggcatca
     8041 tgggcgtggt gggcctcacg ggcgtggtgg tgaacgactc catcgtgatg gtggacttta
     8101 tcaaggtgtc gcggaaccgg gggctctcgc cgctcgcggc gaccctcgag gcgggggcca
     8161 cacggctcag gcccatcttt ctgacgacca tcaccacctt cttcgggctg ctccccaccg
     8221 cctacggcat cgggggctac gatcccttcc tggttcccat ggcgctttcg ctctcgtggg
     8281 ggctggcctt cgggtcgctc atcacgctct tcgcgacgcc ggtgctgtac aatctcttct
     8341 ccgatttcag gcaacgcctg ttccaccgcg acacggagct cttgcggccg gagttcccgc
     8401 cctcgaccat ccaggagatg gagagcgaga tcgaacaggt ggtgcgcgag gacctgaagg
     8461 acgacatgcg cgcggccgtg cgcgatgagg tgcgccgcca gatggaggcg gagcgtgcgg
     8521 agcgcgccgg ccgttcgcgc cgcccgaaca aggggccccg acgataatgg ggcgcgtggt
     8581 gccgcaaccc gcgcccgcat ggagaaagcc atgaaacgac aagcagtcgc cccggtaccg
     8641 cttcgaagcg ccacgcggca ggattgcgtc gcctgcggcc agaggtgctg ccgctcgatc
     8701 tccttcgagg tcgatacgcc cgcgagcccc gaggacttcg accacctgcg ctggatgctg
     8761 gcgcaccaca acgtcttcgt ctacatcgag cacggcgagt ggtacgtgga ggtggccgtg
     8821 gactgcgcga tgctcg
//
LOCUS       DAORHF010000156         8825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7387_length_8825_cov_6.850627, whole genome shotgun sequence.
ACCESSION   DAORHF010000156 DAORHF010000000
VERSION     DAORHF010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8825
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7387_length_8825_cov_6.850627"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="PKJ16_12555"
     CDS             <1..1659
                     /locus_tag="PKJ16_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV47865.1"
                     /translation="AAGNYRSKRRCADTDACTMIMTMEVNSQQGVDVKQGGKRKAGDT
                     EHSALVTINTAGRLASIQFPSNMSDPREQDVLRDLIASWLTTLPEEGDTHAEEKSFFK
                     RMMGRLGLSDERGPFRFASSDTQGEYRGLLTYSGFSEKLLRIAMEKKEYVRSAAPIRV
                     EYSRHEVRWNAEEEVFKSKNGEDRLDIGPEGFVVNTKQQYEFELTHVGVSHFSQRDVR
                     AYSILAKSFYNKNIYHADSGADGPSKRQVRPWNEVQAELVKVTAHDSNDDKHRIFNDL
                     CNHIKRHPDRAIEALEMARGYRSDDDRFQMIMGALSYAGTPEIQAELQQFYRNGKTDG
                     PLDESGKLSVIGAFTMMDAELTGDSVRFLNAVYNSSESRLLRDSAALALAAGHKNNSD
                     HDQDSMRKIVNSEWGRAENDERRMVVLDMIGNSGEKSFLPIISDALRSSNDALRSKAA
                     YAGRFIDTDDGSRLLLNIISGGDVVRVKEKAAEALTYHQWRDSYPPVLSSCVRSGASD
                     SLRMQCAETGLKHKEYHDSMVSILRSARSGASQKFGAYIDRVTQ"
     gene            complement(1718..2383)
                     /locus_tag="PKJ16_12560"
     CDS             complement(1718..2383)
                     /locus_tag="PKJ16_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47866.1"
                     /translation="MKKKKSKSAPKKKTKKKASPVKSAARSATKKPSPAKKAAPAKKK
                     PSGVKKPSTLKKAAARPSSKPKVKSIKKTASIAKKTKPRRKISPKKPQPAPSPIPATP
                     ARTIETPAPPVATPIVPAQPVETRPGADKLSAEITENPSGQSFVITVNNYRNFFALEE
                     MRIIARICQDASSEAEGMLKLHQWFMAFRKDVINNTQIAGASDPALASMYRSVKATYT
                     VKR"
     gene            2628..3407
                     /locus_tag="PKJ16_12565"
     CDS             2628..3407
                     /locus_tag="PKJ16_12565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV47867.1"
                     /translation="MKKAFVFVLAFGFVLAHDALRTSIARGLHGAQARYVWAPSGLNL
                     REKPSPSARVIATLPYGTGVVVVPDAAKPVPYRFEYFSARRGDEGGDGSAEKRAAVRI
                     SGHWVKVDAKGKTGYVVDALLLDVKPKDPKWEVADYLIHVFGMKKVSEKKSTGTCEGG
                     ITHTVESRYESKDKKIVLVTVNKTGEHPCWTIRGEISMTGLSFAKAFVFFNAVLPLFP
                     NYGYTYIEGKKFEYPHDGAGNEATLKKTKDGVSFEWVVAPN"
     gene            complement(3449..5344)
                     /locus_tag="PKJ16_12570"
     CDS             complement(3449..5344)
                     /locus_tag="PKJ16_12570"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HNV47868.1"
                     /translation="MPTIEFQPSGARIDVAPGTELVDAARAAGVAIDIPCGGNGTCGK
                     CLVRIVSGDLNQKKRAEGDDPRLALACQCAIGNDDVVVETFEHHLPAGDRDEDIAEYA
                     SAIATVLRSGRHAPIVQRTALTVPPPRPDDGHSDFDRLKAEIIKQTGKKSVIVPLAVI
                     RALPELLRADDGMVIITWHDIETRAIVIRVEPAAFAAGSYGIAVDVGTTTVAVALVDL
                     ATNAIVAVRSGYNDQIPCGLDVISRINYARRPVRLEDLRGRVVGTINRLLEEIRSKRR
                     VDADAIVAGSLAGNTVMTHLLLGIVPEHLRLEPYTPALLAAPNLSARDAGIAINPDAR
                     LFLSPAVGSYVGGDITAGVLMTPLTGDAEGVSLFLDVGTNGELVIGGRDFLIACACSA
                     GPAFEGGGIDCGMRAADGAIDRVRIADDDLPEFTTIGGASPRGICGSGIIDLIAGLFS
                     KGIIDSSGKFDRSGTNPRIRIDGRRAEYVIESASSSATGADIAISELDIDNVMRAKAA
                     IYSACALMLKQVGMSFTDLERFYIAGGFGRYLNIENAITIGLLPDLPVDRFAYLGNAS
                     LIGSTLALVSKDERERISRSARRMTYVNLSTELDYMDQYTAALFLPHTDRERFPNVYA
                     RLNAKKR"
     gene            complement(5650..6984)
                     /gene="acsC"
                     /locus_tag="PKJ16_12575"
     CDS             complement(5650..6984)
                     /gene="acsC"
                     /locus_tag="PKJ16_12575"
                     /inference="COORDINATES: protein motif:HMM:NF003195.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HNV47869.1"
                     /translation="MALTGLQIQKLLPGTNCRECGSNTCLAFAMKLAAKKAELAECPY
                     ASDEAKQVLGAASEPPVKTIALGADEKVKTGGETVLYRHEKTFVNPTVIAINIDNTDD
                     AAAIEQTLASIRDYRYERVGETFQVDAVAVTQRGGDAKAFIALAEKARTTTQRPIILR
                     SGDTEALALAAKHLAGSHAVICAADPDTALTLMPAARENGHALAITAPDVDSLVALAA
                     KLRENDFANIIIDFSAVSLAERFQLNSIARRAAIKDSFKPLGSPGIRFIDAGDELAIA
                     VEAVTEIAKYGGIVVLPSFDPAELAALMTLRLNLYTDPQKPIQVEPKVYAIGEPTPDS
                     PVFVTTNFSLTYFVVSGEIENSGTSAWLVVPECDGMSVLTAWAAGKFNAEKIAAFMKE
                     AKVEETIRRRDIVIPGYVSQISGELEEALPGWKILIGPQEAADIESFVKQRL"
     gene            complement(6999..7529)
                     /locus_tag="PKJ16_12580"
     CDS             complement(6999..7529)
                     /locus_tag="PKJ16_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HNV47870.1"
                     /translation="MKTDEQIANTIYTIRGHKVMLDRDRAELYEVETRSLNQAVKRNI
                     SRFPGDFMFQLTKDEYDSLRSQFVILKKTEKPQTGKHTKYLPYAFTEQGVAMLSSVLS
                     SERAIQVNIQIMRMFTRLRAMLSTHEDLRRKIESMEKKYDEQFGIVFRAIKRLLSDDA
                     KPKRKIGFVNTTEKNT"
     gene            complement(7561..8400)
                     /locus_tag="PKJ16_12585"
     CDS             complement(7561..8400)
                     /locus_tag="PKJ16_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47871.1"
                     /translation="MPIIGRFVCLGEKAKVGATSACFAKGSLKYYETGQLMSGTMERD
                     RRFMFSSRNCSVIFGSHIIFHRNGTPMNGHATGESWYIDLDGKRVAIYDRFQLHENGM
                     VRFCFFKERRKTQPQAIRVRGTRYDYPEIRTPYALFYDDGSIKGLTLLEDASLFFNTG
                     RHDVEKDTSVIFRRTGEVFALRSSKYNQKTSDNHYVSFSDYQKGVADFHGIFAYQLPK
                     TITVRHKDVDILSGNSETVVFVRYADPAHKRIKSILFIDDLKVEINKKPIDCTAFEWY
                     AVE"
BASE COUNT         1722 a         2500 c         2722 g         1881 t
ORIGIN      
        1 gcagcaggga attatcgctc gaagcgtcgg tgcgccgata ccgacgcatg caccatgatc
       61 atgaccatgg aggtgaactc ccaacagggc gtcgacgtga agcagggcgg gaaacgaaag
      121 gccggcgaca ccgaacattc ggcgctggtg accatcaaca ccgcggggcg gcttgcatcg
      181 atccagttcc cttcgaatat gtccgatccc cgcgagcagg acgtgctgcg cgacctcatc
      241 gcatcgtggc ttaccacgtt gccggaggag ggcgacacgc acgccgaaga aaagtcgttt
      301 ttcaagcgga tgatgggacg cctgggcctt tcggacgagc gtgggccgtt ccggttcgcc
      361 tcgagcgata cgcagggcga gtaccgggga ctgctcacct atagcggctt ctccgagaag
      421 ctcctccgga tcgccatgga gaagaaagag tatgttcgaa gcgccgcgcc gatccgcgtc
      481 gagtattcgc gccacgaggt ccgctggaac gcggaggagg aggtgttcaa atcaaaaaac
      541 ggcgaagacc gtctggacat tgggccggag gggttcgtgg tgaacacgaa acagcagtac
      601 gagttcgagc tcacgcatgt cggggtttcc cactttagcc agcgcgatgt gcgcgcgtat
      661 tccatcctcg cgaaatcatt ttataacaag aatatctacc atgccgactc gggcgcggac
      721 ggcccttcga aacggcaggt tcgcccctgg aacgaggtcc aggccgaact cgtcaaggtg
      781 acggcacatg actcaaacga cgataagcac cggatattca acgacctgtg caatcacatt
      841 aaacgccatc cggaccgggc gatcgaggcc ctggagatgg cgcgtggcta ccggtccgac
      901 gatgatcgct ttcaaatgat catgggcgcg ctatcttatg cgggaacgcc ggagatccag
      961 gcggagctgc agcagttcta tcgtaacgga aagacggacg gcccgctcga cgagagcggc
     1021 aagctcagcg tgatcggggc gttcaccatg atggatgcgg agctgaccgg ggattcggtg
     1081 agatttctta acgcagtgta taattcgagc gagagccgat tgctgcgcga cagcgccgcg
     1141 ctggccctgg cggcgggaca caagaataat tccgatcacg atcaggacag catgaggaaa
     1201 atcgtgaaca gcgagtgggg ccgcgccgag aacgacgagc gccggatggt ggtgcttgat
     1261 atgatcggca atagcggcga gaaatcgttc ctgcccatca tcagcgacgc gctgcgctca
     1321 tcgaacgacg cgctgcgcag caaggccgcc tatgccggcc gattcatcga caccgatgac
     1381 ggcagccggc tcttgctgaa catcatcagc gggggcgacg ttgttcgcgt gaaggaaaag
     1441 gccgctgagg cgctcaccta ccatcagtgg cgggacagct atccgccggt gctctcgtcg
     1501 tgcgtgcgct ccggggccag cgattccctg cgtatgcaat gcgccgagac cggtctgaaa
     1561 cacaaagagt accatgattc gatggtttcc attctgcgct cggcgcggag tggggcgtcg
     1621 cagaagtttg gcgcctatat cgatcgcgta acccaataga cacggacacg ccatgcggcg
     1681 cgtccgtgcg tcgccagcgc ctcaccgacc tcgagtctta ccgcttcacc gtataggtcg
     1741 ccttgaccga tcggtacatg ctcgccagcg ccggatcgga ggcgcccgcg atctgcgtat
     1801 tgttgatgac gtcctttcga aacgccatga accactggtg gagtttcagc atcccctcgg
     1861 cctcgctgga cgcgtcctgg cagatgcgcg caatgatgcg catctcctcg agcgcgaaaa
     1921 aattgcggta gttgttcacc gtaatgacga aggactgtcc gctcgggttc tcggtgatct
     1981 cggccgaaag cttatcggcc cccggccgcg tctcgacggg ctgagcggga acgatcggcg
     2041 tcgctaccgg cggtgcgggc gtttcaatcg tgcgtgcggg tgttgccggg atcggcgatg
     2101 gcgccggctg cggttttttc ggagagattt ttcttctcgg tttcgttttc ttcgcaatgg
     2161 acgcggtctt tttaatggat tttaccttcg gtttcgaact cgggcgagcc gcggccttct
     2221 tcagcgtgga cggttttttt acaccggacg gcttcttctt tgcgggtgct gcttttttcg
     2281 ccggtgacgg tttctttgtg gccgatcggg ccgcactctt tacgggcgac gccttcttct
     2341 tcgttttctt cttcggtgcg ctcttggatt tctttttttt catggcggtc tccgcgcgtg
     2401 tgggttgatg acgtcgtgtt cgctgctctg tctgtatggt atcgagggat agcgtgcgcg
     2461 tcaaataatt tttcatcgtt ccaagcgggg attgtcagtg aagcggcagc atccgaccgg
     2521 ccatgatgga atcgcttgac atgcgcccga gtgtgcgcta tcgagaggat gctccatggg
     2581 gcacgctcgt atccgaggaa tccgttattg ggcggggagg aatcaacatg aaaaaagcct
     2641 tcgtcttcgt tttggcattc ggcttcgtac tggcacatga tgcgctcaga acgtcaattg
     2701 cgcgcggact gcacggagcg caggcgcgtt atgtgtgggc ccccagcggg ttgaatcttc
     2761 gggagaaacc ctcgccctcg gcgcgtgtga ttgcgacgct cccctatggc accggcgtgg
     2821 tcgtggttcc cgacgcggcg aaaccggttc cctatcggtt cgaatatttc tcggcgaggc
     2881 gcggcgacga aggcggcgac ggatcagcgg aaaagcgggc tgccgtgcgg atcagcggtc
     2941 actgggtgaa ggtggacgcg aagggaaaga ccgggtatgt tgtcgatgcg cttctcctcg
     3001 atgtgaagcc gaaggacccc aaatgggaag tcgccgacta tcttatccat gttttcggta
     3061 tgaaaaaagt cagtgagaaa aaatcaaccg gaacctgtga gggcggcata acccacaccg
     3121 tcgagtccag gtacgaatca aaagataaaa agatcgttct cgttaccgtc aataaaacag
     3181 gggagcatcc gtgttggacg atacgcggag agatcagcat gacggggttg tcattcgcca
     3241 aagcgtttgt atttttcaac gccgtgctgc cgctgtttcc gaactatgga tatacgtata
     3301 ttgaagggaa gaagttcgaa tatcctcacg atggtgcggg aaatgaggcg acgctgaaga
     3361 aaacaaagga tggcgtttcg ttcgaatggg tggttgcgcc gaactgaatt ggtttatcga
     3421 agaaatgccg tgtggggtca cggaggtgtc atcgcttctt tgcattaaga cgcgcataca
     3481 cgttgggaaa ccgctcccgg tcggtgtgcg gcaaaaagag cgccgcggta tactggtcca
     3541 tgtagtcgag ctcggtgctg agattcacgt aggtcatgcg gcgcgccgat cgactgatgc
     3601 gctcgcgctc gtcctttgat accagcgcca gcgtcgatcc gatcagagac gcgttgccaa
     3661 ggtacgcgaa acgatcgacg ggaagatcgg ggagcaggcc tatcgttatc gcattttcga
     3721 tgttgagata tcgcccgaac cctcccgcga tatagaaccg ctccagatca gtgaacgaca
     3781 tccccacctg ttttagcatg agcgcgcagg cggagtagat ggcggccttt gcgcgcatta
     3841 cgttgtcgat gtcgagctcg ctgatggcga tgtcggcgcc ggtggcgctc gagctcgctg
     3901 attcgatgac gtactcggcg cgccgcccgt cgatacgtat acgcggattg gtccccgacc
     3961 ggtcgaactt cccggacgag tcgatgatgc ccttcgagaa aagaccggca ataaggtcga
     4021 tgatgccgga accgcagatc ccgcgcggcg aagcgccgcc gatggtcgtg aactccggaa
     4081 ggtcgtcatc ggcgatgcgc acgcggtcga tcgcgccgtc ggccgcgcgc atgccgcaat
     4141 cgatcccgcc gccctcgaag gccggccccg ccgagcaggc gcaggcgatg aggaaatcgc
     4201 gcccgccgat cacgagctcc ccgttggtgc ccacgtcgag aaagagcgac acgccctccg
     4261 catcgccggt aagcggcgtc atcagcacgc cggcggtgat gtccccgccc acatagctcc
     4321 ccacggccgg agacaggaac aaccgcgcgt ccggattgat ggcgatgccg gcgtcgcgcg
     4381 ccgagagatt cggcgccgca agcagcgccg gcgtgtacgg ctcgagccgc aggtgctcgg
     4441 ggacgatccc gaggagcagg tgcgtcatca ccgtgtttcc ggcgagcgat cctgcgacaa
     4501 tcgcgtcggc atcgacccgg cgcttacttc gtatctcttc cagcagtcga ttaatggtgc
     4561 cgacgacccg gccgcgcagg tcctcgagtc gtacgggccg ccgcgcatag ttgatgcggc
     4621 tgatgacgtc gagcccgcag gggatctggt cattgtagcc gctgcggacc gcgacgatcg
     4681 cgttcgtggc gagatcgacg agcgccaccg cgacggtcgt ggtgcccaca tcgacggcaa
     4741 tcccgtatga tcccgccgcg aacgcggccg gctcgacgcg aatgacgatc gcgcgcgtct
     4801 cgatgtcgtg ccaggtgatg atgaccatcc cgtcgtctgc gcgaaggagc tcgggaagcg
     4861 cgcggataac ggcaagcggc acgatgacgc ttttcttccc cgtctgtttg atgatctcgg
     4921 ctttcagccg gtcgaagtcc gaatggccgt cgtcggggcg cggcggcggg accgtaagcg
     4981 cggtgcgctg cacgatgggc gcatgccgcc cggagcgcag gaccgtcgcg atcgccgacg
     5041 cgtattccgc gatgtcctcg tcgcggtcgc cagcgggcag atgatgctcg aatgtctcga
     5101 cgacgacgtc atcgttcccg attgcgcact ggcaggcgag cgcgaggcgc ggatcatccc
     5161 cctccgcacg ttttttctgg ttgaggtcgc cggagacgat gcgaaccagg cacttgccgc
     5221 aggtcccgtt cccgccgcag gggatgtcga tggcgacgcc cgccgcgcgc gcggcgtcga
     5281 ccagctccgt tccgggggcg acgtcgattc gcgcgcctga tggttggaac tctatggtgg
     5341 gcatgtaacc cctatccccc ggcccctttc cccttcgtta ggggaaaggg gggtgcgaga
     5401 ggaaatttca gatttgatta cggtcaatag ccggtcaatt ctgattgtga catcttcatt
     5461 ggaaaaacga atggtgtgta tgcctagact ttcaatatat gaatctcggt agctgtcata
     5521 ctgtttggaa attgcggaat tatggatgca tccggaatac cctcaaagaa taatacgcaa
     5581 caccgagcca ctaattacac ttttcccaaa taacacctcc ccctaacgaa gggggagggc
     5641 gtgagggggt cagagccgct gcttcacgaa gctctcgata tcggccgcct cctggggccc
     5701 gatgaggatc ttccagccgg gaagcgcctc ctcaagttcg ccgctgatct gcgacacgta
     5761 gccggggatc acgatatcgc gccgccggat cgtttcctcg accttcgcct ccttcatgaa
     5821 ggcggcgatc ttctccgcgt tgaacttccc ggcggcccag gcggtgagca cgctcatccc
     5881 gtcgcactcg gggaccacga gccaggcgct ggtgccgctg ttctcgatct cgccggatac
     5941 cacgaaatag gtgagcgaga agttcgtggt cacgaagacc ggcgagtcgg gcgtgggctc
     6001 tccgatcgcg tacaccttcg gctccacctg gatgggcttc tgcgggtcgg tgtagagatt
     6061 gagccgcagc gtcatgagcg cggcgagctc ggccggatcg aacgacggga gcacgacgat
     6121 gccgccatat ttcgcgatct cggtcacggc ctccacggca attgcgagct cgtctccggc
     6181 gtcgatgaag cgaatgccgg gcgagcccag cggcttgaag ctgtctttga tggcggcgcg
     6241 ccgggcgatc gaattcagct ggaatcgttc ggcaagcgaa acggcggaga agtcgatgat
     6301 gatgttcgcg aaatcattct cgcggagctt cgctgcgagc gccacgagcg aatcgacgtc
     6361 cggcgcggtg atggcgagcg cgtggccgtt ctcgcgcgcg gccggcatga gcgtgagcgc
     6421 cgtgtcgggg tccgccgcgc agatgacggc gtgcgatccc gccaggtgct tcgccgcgag
     6481 cgcgagggcc tcggtgtcgc cgctgcggag gatgattggc cgctgcgtcg tcgtgcgcgc
     6541 cttctcggcg agcgcgataa acgccttcgc gtcgcccccg cgctgcgtca cggcgacggc
     6601 atcgacctga aaggtctccc cgacgcgctc gtagcgatag tcacgaatcg atgcgagggt
     6661 ttgctcaatc gcggccgcat cgtcggtgtt gtcgatgttg atggcgatga cggtgggatt
     6721 cacgaaggtc ttctcgtgcc ggtagagcac cgtctcgccc ccggtcttca ccttctcgtc
     6781 ggcgcccagc gcgattgtct tgacgggcgg ctcgctcgca gcgcccagca cctgcttcgc
     6841 ctcgtcggag gcgtacgggc attcggcgag ctcggccttc ttggccgcga gcttcatcgc
     6901 gaaggccagg caagtgttcg atccgcactc ccggcagttg gtgccgggga ggagcttctg
     6961 tatctgcaat ccggtcaggg ccatgtgatt tctcctggtt atgtgttctt ttcggtggta
     7021 ttcacgaatc cgatttttcg cttgggtttc gcgtcatcgg aaaggagccg cttgatggcg
     7081 cggaagacga tgccaaactg ttcgtcgtat ttcttttcca tgctttcgat cttgcgacgc
     7141 aggtcctcgt gcgtcgagag catggcgcga aggcgtgtga acatgcgcat gatctggatg
     7201 ttcacctgga tcgcgcgctc gctgctcaat acgctagaga gcatggccac gccctgctcg
     7261 gtgaaggcgt acggaaggta tttcgtgtgc ttgcccgttt gaggtttctc ggttttcttt
     7321 aagatcacaa attgtgatct taaagaatca tattcatcct tcgttaattg aaacatgaaa
     7381 tcacccggaa aacgggagat gttccgcttc accgcctgat tgagcgaccg ggtctccacc
     7441 tcgtagagct ccgcacggtc acggtccagc attaccttgt ggcctcgaat tgtgtatatc
     7501 gtgttcgcga tctgttcgtc ggttttcatg acggtcctgg cactgttcca aagaagccgg
     7561 ttactcgacg gcgtaccact cgaaggcggt acagtcgatt ggtttcttat tgatttcgac
     7621 cttcaaatcg tcaataaaca atatcgattt tattcgtttg tgcgccgggt ccgcatagcg
     7681 cacgaaaaca accgtttccg agttcccgga taggatgtcg acgtctttgt gacgtacggt
     7741 aatagtttta ggcaactggt aagcgaagat gccatgaaaa tcggccactc ctttttgata
     7801 atcgctgaat gacacataat ggttgtctga cgttttttga ttgtattttg atgaacgcag
     7861 ggcgaacacc tcgccggtcc gccgaaaaat caccgacgtg tccttctcaa cgtcatgacg
     7921 cccggtattg aagaacagcg aagcgtcctc caggagcgtg aggcccttga tcgatccgtc
     7981 atcgtagaac agtgcatacg gggttcgtat ttcggggtag tcatatcgtg ttccccgtac
     8041 gcgtatcgcc tgcggctgcg tctttctcct ttccttgaaa aaacaaaacc ggaccatgcc
     8101 gttttcatga agctggaatc gatcatagat cgcgactcgt ttcccatcga ggtcaatata
     8161 ccagctttcc ccggtcgcgt gcccgttcat aggcgtcccg ttgcggtgga aaatgatatg
     8221 gctgccgaat attacactgc aattacggct ggaaaacata aaacgacgat ctcgttccat
     8281 ggtgccgctc atgagctggc ccgtctcgta gtatttcaac gatcccttgg cgaagcatgc
     8341 ggaggtagcg cccaccttcg ccttctcacc gagacagacg aaccggccga tgatgggcat
     8401 gcaggtgcgg gacatgcagc tgatggggtt gcagtcgtac gtgggtccca tgtgcgccat
     8461 catcggcgcc ggcagcgcaa ggaacacgaa gaaaaatatt gatagagttt tcatgtcgca
     8521 aacgtattgc atcccgctca cccgagtcaa gcgtaatcag tgcgtgaaaa tagcattgac
     8581 gtatttaacc gcaccgctac gctatttatg cagagtgtca tcgcttcgct cgtgatgaca
     8641 ttgagcttag caatttgtct actatcgacg gggctcatta aatgaaaaaa tccatcatca
     8701 cgtactgcgc cgttccggtt atcgttatac tgacgttttt gtccatgctg atggtgtgcg
     8761 ccgatcacga cacacgcgac ggcgggcagg actcccgggc cgtcgcggat gcggagaacg
     8821 cgata
//
LOCUS       DAORHF010000157         8798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7425_length_8798_cov_3.572229, whole genome shotgun sequence.
ACCESSION   DAORHF010000157 DAORHF010000000
VERSION     DAORHF010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7425_length_8798_cov_3.572229"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PKJ16_12590"
     CDS             <1..457
                     /locus_tag="PKJ16_12590"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV47872.1"
                     /translation="ENIRLLREAKYMTITVLACIDNGVFHFAGLHQDILVHRRARGAV
                     EVIETRGFWIGMQDDPDIVHLEQSLTLEPGDTMLLYTDGITEAWRRGTVRDLRDPRTD
                     MFGQERLIGILSHTAPESPDAVKREILAALRDYDCHDDVTMVILRRTGE"
     gene            559..1008
                     /locus_tag="PKJ16_12595"
     CDS             559..1008
                     /locus_tag="PKJ16_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HNV47873.1"
                     /translation="MSIPTTLYIRKDILGRVDALCKKSGMSRATLVNRALRLSMRKNY
                     SPVAGRDAVRYQQSAPADSWRILHVRFGKDEYEFFTDMRKVFKMSVSLLLAYSIMNFY
                     DDIIGTGKDEIKRDNYHFSHHVLTSSTTGTGICWKLYWLKPPNPMKN"
     gene            1140..1595
                     /locus_tag="PKJ16_12600"
     CDS             1140..1595
                     /locus_tag="PKJ16_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47874.1"
                     /translation="MPRAKIYGYLAIIVSLAAAFAFLYFGFYDTIIGRPIPSSASYYA
                     IAIPIGVVTLAVLVTGFWIGWTILTIQVVKPMPEIVEKKDYAKAKAFLLCLATLALAA
                     LFAYGLYLQSFWALAVPAAVITLVILGMVFWVGIAIITTRNTLPADKNS"
     gene            1597..2841
                     /locus_tag="PKJ16_12605"
     CDS             1597..2841
                     /locus_tag="PKJ16_12605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV47875.1"
                     /translation="MRALFLCYRGNPFCGGQGIYLYHLTRELAALGVEIDVIVGPPYP
                     DPLEGWADVYKTENLNIWSIRTRHFGPDKLQRVLSPWNFVDYFLTRMHVFGEMETFSG
                     RAFFKLRDLLRTRRYDIIHDVQCLGWGLVPMRGYGIPIVTTVHHPLTRDREADFTHDK
                     SFWELMTTVLFYPLTMQRFVINRLDRVITSSTEGAEELGRAFGVRRDRISVVYNGMDV
                     EMFRNDGSEREPDTLLFVGNTEDHKKGLIYLFEAMTLLPERITLTIVDEGPPVRTSAF
                     NMIKRFGIEDRVRFTGKVSLDRLVNLYSSKAVLVMSSLYEGFGLPAAEAMACETPVVV
                     TAVGSLPEVVAHNETGLIVPPENPRALADAIMMVLKDKKLQRSMGKKGRMRTVENFAW
                     PVAAQNTLAVYEDVIRAYRRAS"
     gene            2838..4073
                     /locus_tag="PKJ16_12610"
     CDS             2838..4073
                     /locus_tag="PKJ16_12610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV47876.1"
                     /translation="MKICLMSYRGNPYCGGQGVYLMYIARELVKLGHEVHAIVGPPYP
                     FEMQGVTLHRIGNHNYFNVPDFVKPHKPFATFQPFNFYEFVASKLGIFPEIETFSFRA
                     FLKLKELQKTHRFDIIHDNQCLGYGFLLIKNLGVPFVSTLHHPLSIDRTTWFEYPSDF
                     SLRVKRVLYYPLIMQAFVSNRLDRIITVSHDSAKEINLAFGVPMEKQRVVYNGLDSSI
                     FHPLTGVKKKRNQIIFVGNVEDRKKGVAYILKALTLTKHKVHLIVVDGGAPNRRFVPG
                     WIDRFGLNDRIRFMGKVTNEELVRLYAESEIALSPSLYEGFGFPAAEAMACELPVIAS
                     TGGALPEVVGEHLKTAYIVPTRDPEAIAAGIDTLMDDPALRRRMGKAARARILTTFTW
                     ENATKEMVKVYQEAIDAHR"
     gene            4060..4629
                     /locus_tag="PKJ16_12615"
     CDS             4060..4629
                     /locus_tag="PKJ16_12615"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV47877.1"
                     /translation="MLTVDFDWLGLKSGERLLDAGCGEGRHSIEGLIRGARVWGMDMD
                     MPSLLKARFNLGMMAKAKMTHPEGRYLTHIGDARHLPFPDETFDRVICSEVMEHVDDD
                     NRACAEMVRVLKTGGTIAITVPTYFTELNRRAQLGVLPQLARRAHSQVPPRPSCPHHA
                     RERARHLPCGLQARLPFHLLDDPVSGGTA"
     gene            4613..4765
                     /locus_tag="PKJ16_12620"
     CDS             4613..4765
                     /locus_tag="PKJ16_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47878.1"
                     /translation="MVGLHDNNQPITKAYRTFLTLGLYSKLMRRVERFFNWFFPKSVV
                     LYGVKR"
     gene            complement(4789..6654)
                     /locus_tag="PKJ16_12625"
     CDS             complement(4789..6654)
                     /locus_tag="PKJ16_12625"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV47879.1"
                     /translation="MRKRTVITTLIIAIATLNCNNLFHEIPSSGESAGSSTILSAGVH
                     YVYPKNNFSNVPLNTKVLVAFATDMDITTVTSDSIQVEDAIGPVSGSLEFHGDRIVLF
                     KPDVNFSNYTQYAVTLKSGMRDKTGQVIMHDYTFKYVTGEHVDTTAPVVEITSPAENS
                     MVGVSEKYVSAKFNKDIDPKTLTTASFLIQEGINALPGRVVYDASLRTAYFFMDDALA
                     NYTTYTATLQTVVADLCGNTLSMQKSWGFRTGLAPVFVSLEPASNENNGIYTFGNISG
                     TGRILRNADGSRIDSSYMPGQSLADIVDLGTEGAFGNGKSFRVVVNGFNLADAIRGLG
                     LAPRYMNFIDGVINPPLSECYIDLYRGLLVLPRPIYWSRCESIGNLTNAEIGVFGPYV
                     TGSFMGASGVKFGNGVYNFGPGGYGFSTMSGSIYPKGNDIAGFDAGTLSFWGMVSSDA
                     TAGYFTVSAYQSYLNGNISLTMETVSGGSWYDRMVLYVNGSAVVTYGISRGVVYHVYM
                     IWDRSRGLQDGKSIRVFVNGVEAMSSSVVFNSTVGQKPYVTLYGSQANWDDWGGASAH
                     LDNLVIWDHVVSENPSWLYNGGVGRENALHYMYGAPDYRPKLTGPNNGVGYYYLP"
     gene            complement(6785..>8798)
                     /locus_tag="PKJ16_12630"
     CDS             complement(6785..>8798)
                     /locus_tag="PKJ16_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47880.1"
                     /translation="ERPPLLPVWAATALKVGVAGLLFWWIGANAPAEFVEHFTVYLPF
                     AKSLVPGNNAPHAAIMDPVPNPIRANSPIYIRAQAYDWDGGGSIAGVKLSYRKLRPNG
                     SAIPGPWQTVFMNKEVRPNELSIYGFLIPASFLTSADLAAGNDIEFRVEAIDNLGAVT
                     TASRSIDVTRRYQAGTLSFNPASQTIAAGFERNFVLRGRDTNGTMYNLIPEIFALANN
                     YGTIKQQFAAGVLFHAKNTGIETLSAGFGAESASSAISVRNGSLERISILNESGLLLD
                     GDVVLSEGVPCNFDVLGYDEYGNRILVVPAWTADAAIGTINQDGVLNTNGARGTGTVS
                     ATLGAKSASHSVTVYSRAKDITAFSVLGIDGSIDGTNISVTVPAGTLRHSIVSTFTTT
                     GVSVYCGATQLQSGVTQLDMTNPVIFKVFAEDGSWKEYTVTLVNNPADPIYWSRCESI
                     GNLTNAEIGVFGPYVTGSFMGASGVKFGNGVYNFGPGGYGFSTMSGSIYPKGNDIAGF
                     DAGTLSFWGMVSSDATAGYFTVSAYQSYLNGNISLTMETVSGGSWYDRMVLYVNGSAV
                     VTYGISRGVVYHVYMIWDRSRGLQDGKSIRVFVNGVEAMSSSVVFNSTVGQKPYVTLY
                     GSQANWDDWGGASAHLDNLVIWDHVVSENPSWLYNGGAGVEDAMHP"
BASE COUNT         2073 a         2631 c         2212 g         1882 t
ORIGIN      
        1 cgagaacatc cggctcctgc gcgaagcgaa gtacatgacc atcaccgtgc tggcgtgtat
       61 cgacaacggc gtgttccatt tcgccgggct gcaccaggac atcctggtcc accgccgcgc
      121 gcgcggagcg gtcgaggtga tcgagacgcg cggattttgg atcggcatgc aggatgatcc
      181 ggacattgtc catctcgaac aatcgctcac gctcgagccg ggagacacaa tgctcctgta
      241 caccgacggc atcaccgagg catggcgcag gggaacggtc cgcgaccttc gcgacccgcg
      301 caccgacatg ttcggacagg agcgactgat cgggatcctt tcgcacactg cgccggagtc
      361 gcccgacgcg gtgaagcgcg agatcctcgc cgcgcttcgc gactacgact gccacgacga
      421 tgtgacgatg gtgatcctcc gccgcacggg cgaatgacat acgcacatca acagcgatag
      481 aaaaaatcaa aaatcccgaa ttgatgaacg taatactcca tggggccaca tgccctcatc
      541 tgacccatgg aggcatccat gtcgatcccc acaaccctgt atatccgaaa agacattctt
      601 ggtcgcgttg atgcgttgtg caaaaaatcc ggaatgtccc gcgccacgct cgtgaatcga
      661 gccctgcggt tgtccatgcg aaagaactat tcacccgtag cgggcaggga cgccgtgcga
      721 tatcaacaat cggctccggc ggattcttgg cgcatcctgc atgttcgttt cgggaaggat
      781 gaatatgagt ttttcaccga catgcggaag gttttcaaga tgtcggtgtc tcttcttttg
      841 gcgtattcca taatgaattt ttacgatgat atcattggaa caggcaagga cgagatcaag
      901 cgcgataact atcacttttc tcatcacgtt ttaacatctt caaccacagg gaccggcatc
      961 tgctggaaat tatactggct aaaacccccc aacccaatga aaaactgacc atatcatcgt
     1021 ataccaccaa cgccgtatcg acctgcgcca aaagatattg acatccggcc gccccggact
     1081 actctggccg caccgtgccg ccccccgccg gcgcgcacct tacgcctgag gatatcatca
     1141 tgccgcgcgc gaaaatatac gggtaccttg ccatcatcgt ttcccttgcc gctgccttcg
     1201 cctttctcta tttcggattt tacgatacca tcatcggccg cccgattccc agttccgcat
     1261 cgtactatgc catcgccatt cccatcggcg tggtgaccct cgcggtgctc gtcaccgggt
     1321 tctggatcgg ctggacaatc ctaaccatac aggtggtcaa acccatgccg gaaatcgtcg
     1381 agaaaaaaga ctacgcgaag gccaaggcct tcctgctgtg cctggcgacg ctcgcgctcg
     1441 cggcgctgtt cgcctacggg ctgtacctgc agagcttctg ggccctcgcc gtccccgcgg
     1501 ccgtcatcac cctggtcatc ctggggatgg ttttctgggt ggggatcgcc atcatcacca
     1561 cgcgcaacac cctgccggcc gacaagaact cgtagcatgc gcgccctctt cctgtgctac
     1621 cgggggaacc cattttgcgg ggggcagggg atatacctgt atcacctaac gcgtgagctc
     1681 gccgcactcg gcgtcgagat cgacgtgatc gtgggcccgc cctacccgga tccgctcgag
     1741 ggatgggcgg acgtgtacaa aacggaaaac ctcaatatct ggtcaatccg caccagacac
     1801 ttcggccccg acaagttgca gcgtgtgctc tcgccgtgga acttcgtcga ttattttctc
     1861 acacggatgc acgtcttcgg cgaaatggaa accttcagcg ggcgcgcctt cttcaaactt
     1921 cgcgaccttc tacgcacgcg gcgctacgac atcatccacg atgtgcaatg cctgggctgg
     1981 ggccttgtcc cgatgcgcgg ctatggcatc cccatcgtga ccaccgtgca ccacccgctc
     2041 acgcgcgacc gcgaggccga cttcacgcac gataaaagct tctgggagct catgaccacc
     2101 gtcctcttct atccgctcac catgcagcgc ttcgtcatca accgcctgga ccgcgtgatc
     2161 acctcgtcga ccgagggggc cgaggagctc gggcgcgcct tcggggtgcg tcgcgacagg
     2221 atatcggtgg tgtataacgg catggacgtc gagatgttcc gcaacgacgg ctcggagcgc
     2281 gaaccggaca cgctgctgtt cgtgggcaac accgaggacc acaagaaggg gctgatatat
     2341 cttttcgagg cgatgacgct cctgccggag cgaatcaccc tcactatcgt tgatgagggg
     2401 ccgcccgtgc gcacgagcgc gttcaacatg atcaagcgct tcggcatcga ggaccgcgtg
     2461 cgcttcaccg gcaaggtgtc cctcgaccgc ctggtgaacc tttacagctc aaaggcggtg
     2521 ctggtgatgt cgtcgctcta cgagggcttc ggccttccgg cggcggaggc gatggcctgc
     2581 gagacgccgg tggtggtcac cgcggtcggg tcgctgcccg aggtggtcgc gcacaacgag
     2641 accggcctca tcgtcccgcc ggagaatccg cgggccctgg ccgacgcaat catgatggta
     2701 ttaaaagata aaaaactcca gcgttcgatg gggaaaaaag gacgcatgcg caccgtggag
     2761 aatttcgcct ggccggtcgc cgcgcaaaac acgctcgcgg tgtacgagga cgtcatccgc
     2821 gcctaccgga gagcctcatg aagatatgtc tgatgagcta tcgcggcaac ccgtactgcg
     2881 ggggacaggg cgtgtacctc atgtacatcg cgcgcgagct cgtaaagctt ggccacgagg
     2941 tgcacgcgat cgtgggaccg ccctatccct tcgagatgca gggcgtgacg ctccatcgta
     3001 tcggcaacca caactacttc aatgttcccg atttcgtgaa gccgcacaag cccttcgcca
     3061 ccttccagcc gtttaacttc tatgaatttg tcgcatcgaa gctcggcatc ttccccgaga
     3121 tagagacctt ctcgtttcgc gccttcctca agctgaagga attacagaag acgcatcgct
     3181 tcgacatcat ccacgacaac cagtgtctcg ggtacggctt tctgctcatt aaaaacctgg
     3241 gcgttccctt cgtatcgacg ctccaccatc cgttgagcat cgaccgcacc acatggttcg
     3301 agtacccatc tgacttttca ctccgcgtga aacgcgtgct gtactatccg ctcatcatgc
     3361 aggccttcgt ctccaatcgg ctcgaccgca tcatcaccgt gtcgcacgat tccgcgaagg
     3421 agatcaacct cgccttcggc gtgcccatgg agaagcagcg cgtggtgtat aacggcctcg
     3481 actcgtcgat ctttcatccg ctcacgggcg taaagaagaa acggaaccag atcatcttcg
     3541 tcggcaacgt ggaagaccgc aagaaaggcg tcgcctacat actgaaggcg ctcacactca
     3601 ccaaacacaa ggtacacctg attgtcgtcg atggcggcgc gcccaaccgt cgcttcgttc
     3661 ccggctggat cgaccgcttc ggcctcaatg accgcatccg cttcatgggc aaggtcacta
     3721 acgaggagct cgtgcggctc tacgccgaat ccgagatcgc gctctcgccg tcgctttacg
     3781 agggcttcgg cttccccgcg gccgaggcga tggcctgcga gctccccgtg atcgcctcca
     3841 ccggcggcgc gcttcccgag gtcgtgggcg agcacctgaa gacggcctac atcgtcccca
     3901 cgcgcgaccc cgaagcgatc gcggccggca tcgacacgct catggacgat cctgccttgc
     3961 gccggcgaat ggggaaggcg gcacgcgcgc gcattctcac aacctttacc tgggagaacg
     4021 ccacgaagga gatggtgaag gtctaccaag aggcgatcga tgctcaccgt tgatttcgac
     4081 tggctggggc tgaaaagcgg cgaacggctt ctcgacgcgg gctgcggcga ggggcgccac
     4141 tccatcgagg ggctgatccg cggcgcgcgc gtctggggaa tggacatgga catgccctcc
     4201 cttctcaagg cgcgattcaa cctggggatg atggcgaagg cgaagatgac ccacccggag
     4261 gggcgctacc tcacgcacat cggcgacgct cgtcacctgc ccttccccga cgagaccttc
     4321 gatcgcgtca tctgctccga ggtcatggag cacgttgacg acgacaaccg cgcctgcgcc
     4381 gagatggtgc gtgtactcaa gaccggcggc accatcgcca tcaccgtccc cacctacttc
     4441 accgagctta atcgacgtgc tcagctggga gtacttccac aactcgcccg gcgggcacat
     4501 tcgcaagtac ctccccggcc gtcttgcccg catcatgcgc gcgaacgggc tcgacatcta
     4561 ccatgtgggc ttcaagcacg ccttccattc catctactgg atgatccggt cagtggtggg
     4621 actgcatgac aacaaccagc ccatcacgaa ggcctatcgc acctttctca cgctgggact
     4681 ctactcgaag ctcatgcggc gcgtggagcg attcttcaat tggttttttc caaagagcgt
     4741 tgtgctgtat ggggtgaagc ggtaatcgag catcctcaca aacgagtgtc aggggaggta
     4801 gtagtagccg acaccgttat tggggcctgt cagctttgga cgatagtcgg gggcgccgta
     4861 catgtagtgg agggcgtttt cgcggccgac accaccattg tagagccagg acgggttttc
     4921 ggagacgacg tggtcccaga tgaccaagtt atccaaatgc gcactcgctc caccccagtc
     4981 gtcccaatta gcctggcttc catatagtgt aacatagggc ttctggccga ctgtagaatt
     5041 gaatactact gacgaactca tcgcctctac cccgtttaca aatacacgta tcgatttccc
     5101 atcctgaagc ccccttgatc gatcccaaat catatacaca tgataaacca caccccgact
     5161 aatcccatac gttactacag cagacccatt tacatataga accatacgat cataccatga
     5221 accacccgaa acggtttcca ttgttaatga aatgttgcca ttgagatatg attgatatgc
     5281 agaaacagta aaatatcctg ccgtggcatc agaactgacc ataccccaga acgaaagcgt
     5341 tccagcatca aatccagcaa tgtcattacc cttgggatat attgaaccac tcattgtaga
     5401 aaaaccatat ccgcctggcc caaaattata aacaccattg ccaaatttca cacccgaagc
     5461 ccccataaat gaaccagtca cataaggacc aaatacacca atctcagcat tggtcaaatt
     5521 cccaatactc tcacatcgac tccaataaat cggccttggt aaaaccaaca gccccctata
     5581 taaatcaata tagcattctg ataatggcgg attaatgaca ccgtcgataa aattcatata
     5641 ccttggggcc aatcctaaac ctctaatagc atcagcaaga ttaaagccat tgacgacgac
     5701 tctgaagctt ttaccgttac caaatgcccc ttccgttcct aaatcaacaa tatcagcaag
     5761 cgactgcccg ggcatatacg aggagtctat cctgcttcca tcagcattcc gcaaaatcct
     5821 gccggtaccg cttatatttc cgaatgtata gattccattg ttctcattcg atgcgggctc
     5881 caaagaaaca aacactggcg ccaacccggt acgaaaaccc catgatttct gcattgatag
     5941 tgtatttccg caaagatcag cgacaaccgt ttgtaacgtt gcggtgtacg ttgtatagtt
     6001 tgcaagtgcg tcatccataa aaaaataggc tgttcggagt gacgcgtcat aaacgaccct
     6061 tccgggaaga gcatttattc cctcttgaat caaaaatgaa gcggtcgtta gcgtttttgg
     6121 atcaatatcc ttattgaact tcgccgaaac gtacttttcg gacactccaa ccatagaatt
     6181 ctcggctggg ctggttattt caaccaccgg ggcagtcgta tcaacatgct ccccggtaac
     6241 atacttgaaa gtataatcgt gcatgataac ctgaccggtc ttatcacgca tgccgctttt
     6301 cagtgtaacg gcatattggg tgtaatttga gaagttaaca tcgggtttaa aaagcacaat
     6361 acgatcccca tggaactcga gggagccgct caccggccca attgcatcct cgacttgtat
     6421 tgaatcgctt gtaacggtcg taatatccat gtccgttgca aaagcaacaa gaacctttgt
     6481 gtttaacgga acattgctga aattattctt tggatataca taatgcacgc ctgcagaaag
     6541 tatggttgac gaacctgctg attcaccgga agagggtatt tcgtgaaaga gattattgca
     6601 attgagcgtg gcaattgcaa taatcaatgt tgtaataacc gttcgttttc tcattgacat
     6661 tgcaaatagt gaacacaatg aataattgca cggaaatgct ctcgcttgtc aagttgtata
     6721 tttgaaaaaa ctgaccagcc atattattta cctcatggct ggccagttaa tactgtgata
     6781 aaacttatgg gtgcattgcg tcctcaaccc cggcgccacc attgtagagc caggacgggt
     6841 tttcggagac gacgtggtcc cagatgacca agttatccaa atgcgcactc gctccacccc
     6901 agtcgtccca attagcctgg cttccatata gtgtaacata gggcttctgg ccgactgtag
     6961 aattgaatac tactgacgaa ctcatcgcct ctaccccgtt tacaaataca cgtatcgatt
     7021 tcccatcctg aagccccctt gatcgatccc aaatcatata cacatgataa accacacccc
     7081 gactaatccc atacgttact acagcagacc catttacata tagaaccata cgatcatacc
     7141 atgaaccacc cgaaacggtt tccattgtta atgaaatgtt gccattgaga tatgattgat
     7201 atgcagaaac agtaaaatat cctgccgtgg catcagaact gaccataccc cagaacgaaa
     7261 gcgttccagc atcaaatcca gcaatgtcat tacccttggg atatattgaa ccactcattg
     7321 tagaaaaacc atatccgcct ggcccaaaat tataaacacc attgccaaat ttcacacccg
     7381 aagcccccat aaatgaacca gtcacataag gaccaaatac accaatctca gcattggtca
     7441 aattcccaat actctcacat cgactccaat aaatcggatc cgcaggatta ttaaccagcg
     7501 ttaccgtata ctccttccac gacccatcct ccgcaaagac cttgaagata acgggattgg
     7561 tcatgtcgag ctgtgtgacc ccgctctgga gctgcgtggc gccgcaatac acacttacgc
     7621 cggttgtcgt gaacgtggag acgatgctat ggcgaagcgt accggccgga acggtcacgc
     7681 tgatattcgt gccgtcaata ctcccatcga tccccagaac cgaaaatgcc gtgatgtcct
     7741 tcgcgcggct atacaccgtg accgaatgcg acgccgattt cgcgccgagt gtcgccgata
     7801 ccgtgcccgt accccgcgca ccgttcgtat tcagcacgcc atcctggtta atggtgccaa
     7861 tggcggcatc cgccgtccat gcagggacaa cgagtattct gttgccgtat tcatcgtacc
     7921 cgagaacgtc gaagttgcac ggaacgccct cggagagtac gacatcgcca tcgaggagaa
     7981 ggcccgattc attcaagatt gaaatgcgct cgagcgatcc gttacgaacc gatatggccg
     8041 acgacgcgct ttcggcgcca aatcccgcgc tcagtgtttc gatgccggtg ttcttcgcgt
     8101 ggaacaggac gcccgcagcg aactgctgct ttatcgtccc gtaattgttt gccagagcga
     8161 agatttccgg gatgagattg tacattgtcc cgttagtatc gcgaccgcga agcacgaaat
     8221 tacgctcgaa acccgcggcg atcgtctgag aggcaggatt gaacgacagc gtccccgcct
     8281 ggtagcgacg ggttacgtca atggatcggg aggccgtcgt tacggcgccc agattgtcaa
     8341 ttgcctccac cctgaattcg atatcattac ccgccgcgag gtccgcggac gtcaggaacg
     8401 acgctggaat gagaaatccg taaattgaaa gctcgttggg gcggacctcc ttattcatga
     8461 acaccgtctg ccacggaccg gggatcgcac tgccgttggg gcgcagcttg cgataggaaa
     8521 gcttcacgcc ggcgatggat ccgccgccgt cccagtcata ggcctgggca cgaatataaa
     8581 tgggtgaatt ggcccggatg ggatttggca ccgggtccat tatcgccgca tgcggggcat
     8641 tattgcccgg aaccagcgat ttcgcgaacg gcaaatacac cgtgaaatgc tccacgaact
     8701 cggcgggcgc gttcgctccg atccaccaga acagcaggcc ggccacaccg actttcagcg
     8761 cggtcgcggc ccagacgggc agcaagggcg gcctttcc
//
LOCUS       DAORHF010000158         8702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7561_length_8702_cov_5.426969, whole genome shotgun sequence.
ACCESSION   DAORHF010000158 DAORHF010000000
VERSION     DAORHF010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8702
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7561_length_8702_cov_5.426969"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="PKJ16_12635"
     CDS             <1..254
                     /locus_tag="PKJ16_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47881.1"
                     /translation="TPLEEVARREKETALRPFLFIARGIARHLGVDDAEARYQTTRLW
                     TELNGIVSLYNSRLLREVTEDVDALTARLAEDICERMRE"
     gene            308..3301
                     /locus_tag="PKJ16_12640"
     CDS             308..3301
                     /locus_tag="PKJ16_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV47882.1"
                     /translation="MLLYTDIQAKSGKSTTGLIMRAFLRFVAANLNRRASLRRYLACA
                     DGWLDFSVGMRTESGSVECGIVGRDGVVTASGRVPDDADAVVVFRTDAAARRLLTGTP
                     TDQIYMLLTSEMRVEGSIMYLNLLSFFLSLVFGRMQRSAMERERRTDRKKLLRKSPRA
                     RQDLRDGLVVRKAQHLRCAAREPGVRFLADPYLPEYSLDDFPRLRKFLDIHLTEKPEV
                     CPEIPRLLTRWHREHGFEQDASGREWEPVLRKAHAYKYLMENREAIIGEDSLIAGTTT
                     SKRIGCPVYPDGSGGLIWNELLTISHRTHNPFVISDETREFLHNDAFPYWMKRNFREW
                     VRDTYGNPLCQQLDERYAFYFNWKQATISHTIPDFPKMLKIGAGGIIREIRRELGKRC
                     DREKKATLEAMILCLEGLIAYGRNLSKRASQDALREPNPARRDELERLAAICARVPET
                     GARTLDEAVNSLWITWVALHMESMNAGLSLGRLDQWLQPYFAADMKKLKAKEKRAAYV
                     KRAIELIGHLFMRCTDHFPLTPDLANFYFGGSSSDQAITLGGVTPRGGDAVNDMTYVF
                     LKVTEMLSLRDPNVNARYMPGVNSDAYLKRLCEVNLSTAATPSLHNDAAVIASLEKMG
                     YDIRDIRDWSATGCVEPTLSHRHMGHTNMQMMNMVAALEMALNDGRHPLTGLEIGPKT
                     GDPLRGEITSFEEFFSAFTAQLAFLVDSSIEYNHMLAEAHQRIRPTPLLSALIDGCVR
                     KGRDATKGGATYNSSGTALIGLADVTDSLMAVKKLVFEEGVITLPELKRAVDANFKND
                     PALLALARRKVPLFGSGSDEAVAMANRVAKWAHDHYASLPHYRGGTYTTGFWSMSNHV
                     AFGTLTGALPSGRLAGTPFTPGLTPQPFASKSLLDNIRDVARLDPANLNNNIAFNVKV
                     VPSAGDSREKTVDDMYSYVKTFFELGGMQMQLNVVTSETLRDAMAHPEHYRNLIVRIS
                     GYNAYFVTLNRDMQMELIERAEYGI"
     gene            3298..4206
                     /locus_tag="PKJ16_12645"
     CDS             3298..4206
                     /locus_tag="PKJ16_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNV47883.1"
                     /translation="MNDPLILEIRGNSLDDGPGIRSVVFFKGCPLSCVWCHNPESKRA
                     AAELSFDPDQCVACNTCLARCERTALARKRPGFIDRNKCARCFACADACPSGALSRVG
                     RAMRVSEVAEAVLRDLPFFRNSGGGVTLSGGEPTLFMDFSAELLLTVRRQRVHTLLET
                     CGLFDWDEFSRTMLPHLDAVFFDIKLMDTAAHRRQCGVPNELILANFTRLLECGGTPA
                     VLPRTPLVPGITDTDENLSAIADFLLGLGVSRAALLPYNPMWHGKSAKIGSSDPTAKD
                     AALAAWQDTATIERCRAIFTRRGIAV"
     gene            complement(4241..4633)
                     /locus_tag="PKJ16_12650"
     CDS             complement(4241..4633)
                     /locus_tag="PKJ16_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HNV47884.1"
                     /translation="MKRILAACSALAVSGVIVFATGAAIADTDTYINKNSGPCADDVR
                     RYCRDVKPGAGRIWSCLKGYETDLSPRCRTFRVEQREKMREFHAACRTDVSRYCGGVP
                     AGKGNIIACLRTKKANLSQSCMSFFERN"
     gene            complement(4872..6878)
                     /locus_tag="PKJ16_12655"
     CDS             complement(4872..6878)
                     /locus_tag="PKJ16_12655"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV47885.1"
                     /translation="MPAMTETTSDRIIAHLKKTAIFSQLPDDDLQVMAQYAGFVDYRK
                     GEVIFEKGSKSKQLFIIDRGEVAINSTLDTDRLGQAPSGDQAIARFIGGEIFGEFEFF
                     EGDTRTAFASAAEDTTILVFPREGVNLDHLFDEYAHIFVKIYHTLITVNSSRQRLTHR
                     LISEKTGWIEELKKQMFFDKLTGVYNRTYLEDELARNFAFLGPVFSLLVVKPDNFKLI
                     NDTFGHEAGDGALQAISSKLQSLLRDADFAVRYRGNEFIVVFPSTPLDEAEKIAGVFL
                     REMTAFDIGIAMKRESLFQTFSIGVATYPEHERAFLELVEKAFTKVFEQREKGGNGVR
                     VASAVEDELYNFLKTVGMLSSLKMSELHQFSQHLELLRFPAGAFICRQLDPGDELFII
                     KSGVTSVSISVQDGTEKELTQLKSGEFFGEMAIFENAPRSANCVALEDCEILRMNKED
                     FFTLMRTYPHTAINVMKSMLNKTTDRLNASGQFITQMVKWGEDASLRAVTDKLTGVFN
                     RRYLESELEKRFTMAQKEGAPLSLVMADMDFFREVNEGYSHEVGDRYIVEVAKVFTTT
                     FRKTDIVSRYGGDEFTILLPNTDLETAMAVTEDVRRAVERLDFLKELEGPDLHLSVSL
                     GVSCFPATARTQAELTEQADKALYAAKKGGRNRVEHFAIRGPEA"
     gene            complement(7003..8448)
                     /locus_tag="PKJ16_12660"
     CDS             complement(7003..8448)
                     /locus_tag="PKJ16_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV47886.1"
                     /translation="MAHSPLTEIMNARSIAFYGASNNVSKMGSSQLVTMISNGYPGAI
                     YPIHPSEETVFGLRAYRSVGEIGTPVDLAVIVLPARLVPGVIDELGAAGVRRAVIVTA
                     GFAEVGGADLQDAVNEAAHRAGLRYVGPNCIGVANLHARLNFTVFPFAGEPGSIGMLS
                     HSGTFVTHLMPYLHARRLNYAEAISLGNEGDLDIVDGLQYYASRDQVRVITCYIEAIR
                     RGREFMATAADVSRVKPIVALYAGGTDAGARATASHTAAMAAPEGIIDGLFRQTGIVR
                     ASTIEEVFDFACVLSRCAVPRGNRVGILTNSGGPGVLMADAVSRLGLSLPSFSPALQK
                     EIGAFAPGTASLANPVDFTFINDWEAFFSRVPRLIAESGEVDVLLFYGFFGIETFLHY
                     RDHPAVAPSLNPALMEAALRLGTDLQHTIAASLRNLPVPIICSTFLGLDESLIGHLVE
                     SGVPFLPTPERAARAAGALCRYGQWRRTHPE"
     gene            complement(8451..>8702)
                     /locus_tag="PKJ16_12665"
     CDS             complement(8451..>8702)
                     /locus_tag="PKJ16_12665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47887.1"
                     /translation="SCRIYETRPAICREHGVEMEPAVLCEGLQDVIATYERYFTGIDE
                     LEAFIPGYLEGLKKKKGAARRTLDAVRRAVRPSSHTKRK"
BASE COUNT         1513 a         2825 c         2829 g         1535 t
ORIGIN      
        1 ggacgcccct ggaggaggtg gcgcgccgcg aaaaggaaac cgccctgcgt ccgtttctgt
       61 tcatcgcacg ggggattgcg cggcatctgg gggtcgatga cgccgaggcg cgctatcaga
      121 ccacgcgcct ctggacggag ctcaatggca tcgtgagcct gtataacagc cgactgctgc
      181 gcgaggtgac cgaggacgtc gatgcgctca cggcgcgtct ggccgaggat atctgcgaac
      241 ggatgcggga gtgatcgcga ttagcacggc aacgcgcgtt tatcacacaa cgtggaggtg
      301 atgcatcatg ctgctctaca cggacattca agcaaagtcg gggaaatcga ccaccggtct
      361 catcatgcgg gcgttcctgc ggttcgtggc ggcgaacctg aaccggcgcg cctcattgcg
      421 ccgctatctg gcctgcgccg acggctggct cgacttctct gtggggatgc gcaccgagag
      481 cggctcggtg gaatgcggca tcgttggccg cgacggcgtg gtgaccgcat ccgggcgcgt
      541 ccccgacgat gccgacgccg tcgtggtgtt tcgcaccgac gccgcggcgc gccggctcct
      601 cacgggaacg cccacggacc agatctacat gctcctgacc agcgagatgc gcgttgaggg
      661 gagcatcatg tatctcaatc tcctttcgtt tttcctttcg cttgtgttcg gccgcatgca
      721 gcggagcgcc atggaacggg agcgtagaac ggaccgcaaa aaactcctgc ggaaatcgcc
      781 gcgtgcgcgg caggatctgc gtgacggcct cgtcgtgcga aaggcccaac acctgcgatg
      841 cgccgcgcgc gagcccgggg tgcgcttcct tgcggacccc tatctccccg aatattcgct
      901 cgatgacttc ccccggctca ggaaatttct ggacatacat cttaccgaaa agcccgaggt
      961 gtgcccggag attccgcggc ttctcacgcg ctggcaccgc gaacacggct tcgaacagga
     1021 tgcatcgggt cgcgaatggg agcccgtcct ccgaaaggcg catgcctaca agtatctcat
     1081 ggaaaaccgg gaggcgatca tcggtgagga ctccctgatc gccggcacca ccacgagcaa
     1141 gcgcatcggc tgccccgtgt accccgacgg gagcggcggg ctcatctgga acgagctcct
     1201 gaccatttcg caccgaaccc acaatccctt cgtcatttcc gacgagacgc gcgagttcct
     1261 gcacaatgac gccttcccct actggatgaa gcggaatttc cgcgagtggg tccgtgacac
     1321 gtacggcaac ccgctctgcc agcagctgga cgaacgctat gcgttctatt tcaactggaa
     1381 acaggcgacc atctcgcaca ccattcccga tttcccgaag atgctcaaga tcggcgccgg
     1441 cggcatcatc cgcgagatac gccgcgagct cgggaagcgc tgcgatcgcg agaagaaggc
     1501 gacgcttgaa gcgatgattc tctgcctgga ggggctgatc gcctacggcc ggaacctgtc
     1561 gaagcgggcg tcgcaggacg cgctgcgcga gccaaacccc gcgcggcgcg acgagctcga
     1621 gcggctcgcg gcgatctgcg cgcgcgtccc ggagacgggc gcgcgcacgt tggacgaagc
     1681 ggtaaattcc ctgtggatca cctgggtggc cctgcacatg gagagcatga acgcggggct
     1741 ctcgctggga cgattggacc agtggctcca gccgtatttc gcggcggaca tgaagaagct
     1801 gaaagcgaag gagaaacggg cggcgtatgt gaagcgcgcc atcgagctca tcgggcacct
     1861 cttcatgcgc tgcacggacc acttcccgct cacgccggac ctcgcgaact tctacttcgg
     1921 cgggagctcg tcggaccagg ccatcacgct gggcggcgtc acgccgcgcg gcggcgacgc
     1981 ggtgaacgac atgacctatg tctttctgaa ggtcaccgag atgctctcgc tgcgcgaccc
     2041 gaacgtgaac gcgcgctata tgcccggcgt gaacagcgac gcctacctga agcgcctgtg
     2101 cgaggtgaat ctgtccacgg cggcaacgcc gtcgctgcac aacgacgccg ccgtcatcgc
     2161 gtcgctggag aagatgggat acgatatccg cgacatccgc gactggtcgg ccaccggttg
     2221 cgtggagccc acgctctcgc accggcacat gggccacacc aacatgcaga tgatgaacat
     2281 ggtcgccgcg ctggagatgg cgctcaacga cggacgccac cccctcacgg ggctcgagat
     2341 tggcccgaaa acgggcgatc ccctgcgcgg cgagattacc tccttcgagg agttcttctc
     2401 ggccttcacc gcgcagctcg cctttctcgt cgattcatcc atcgaataca accacatgct
     2461 cgccgaggcg catcagcgga tccgccccac gccgctgctc tcggcgctca tcgacggatg
     2521 cgtgcgcaaa gggcgcgacg cgacgaaggg cggcgcgacc tacaacagct ccggcaccgc
     2581 gctcatcggg cttgccgacg tgacggactc gctcatggcc gtcaagaagc tcgtcttcga
     2641 ggagggcgtc atcaccctcc ccgaattgaa acgggcggtc gatgcgaatt tcaaaaacga
     2701 ccccgcgctc ctggcgctgg cgcgccggaa ggtgcccctg ttcgggtccg ggagcgacga
     2761 ggccgtggcc atggccaatc gagtcgcgaa gtgggcgcac gaccattacg cgagcctgcc
     2821 gcactaccgc ggcggcacat acaccacggg gttctggtcc atgtccaacc acgtggcctt
     2881 cgggacgctc acgggggcgc ttccctctgg ccgccttgcg gggacgccct tcacgccggg
     2941 actcacgccg cagcccttcg catcgaagag cctgctggac aatatccgcg acgtggcgcg
     3001 gctggacccg gcgaacctga acaacaacat cgccttcaac gtgaaggtgg tgccgtcggc
     3061 gggcgattcg cgggagaaga cggtggacga catgtactcg tacgtgaaaa ccttcttcga
     3121 gctgggcggg atgcagatgc agttgaacgt ggtgacctcg gagacgctgc gcgacgcgat
     3181 ggcgcacccc gagcactacc gcaatctcat cgtgcgcatc tccgggtaca acgcctattt
     3241 cgtcacgctc aaccgcgaca tgcagatgga gctcatcgag cgcgcggagt acgggatatg
     3301 aacgacccgc tcatcctgga gatccggggg aactcgctcg acgacggtcc cggcatccgc
     3361 tccgtcgtgt tcttcaaggg ctgcccgctc tcctgcgtgt ggtgccacaa tccggagagc
     3421 aagcgcgccg ccgcggagct ctcgttcgat ccggaccaat gcgttgcctg caatacctgt
     3481 ctggcgcggt gcgagcgcac ggccctcgca agaaagcgcc cgggcttcat cgaccggaac
     3541 aaatgcgcgc gctgcttcgc ctgcgccgac gcctgcccgt cgggggcgct ctcccgggtg
     3601 gggcgcgcca tgcgcgtgtc tgaggtcgca gaggcggtgc tccgcgatct cccctttttc
     3661 cgcaactcag gcggcggggt gacgctctcc ggcggcgagc cgacgctctt catggacttt
     3721 tccgcggagc tcctgctaac agtgcggcgt cagcgcgtcc acacgctcct ggagacctgc
     3781 ggactgttcg actgggacga gttctcgcgc accatgctgc cgcatctcga cgcggtgttt
     3841 ttcgacatca agctcatgga cacggccgcg caccgccgcc agtgcggcgt ccccaacgag
     3901 ctgatactcg ccaattttac gcggctgctg gaatgcggcg ggacgccggc cgtgctgccg
     3961 cgcacgccgc tggttccggg catcaccgac accgacgaga acctttcggc gatcgcggat
     4021 ttcctgttgg gattgggcgt gtcccgggcc gcgcttctgc cctacaatcc gatgtggcac
     4081 ggaaagagcg cgaagatcgg aagcagcgac ccgaccgcga aggatgcggc cctggcggcc
     4141 tggcaggaca ctgccacgat cgagcgctgc cgcgccatct tcacccgccg gggcattgcc
     4201 gtctaacgcg cggtgcgccg tccgcccctg attgcggcga tcagttccgc tcgaaaaaac
     4261 tcatgcagga ctggctcaag ttcgccttct tcgtccgcaa acacgcgatg atgttgccct
     4321 tccccgcggg gacgccgccg cagtagcggc tcacgtccgt tcggcaggcg gcatggaact
     4381 cgcgcatctt ctcgcgctgc tccacgcgaa aggtccggca tcgcggcgag aggtccgttt
     4441 cgtacccctt gaggcacgac cagatgcggc ccgcgccggg cttcacgtcg cggcaatagc
     4501 gccggacatc atcggcgcag ggtccgctgt ttttgttgat ataggtatcg gtatcggcga
     4561 tggcggcgcc cgtcgcgaac acgatgacgc ccgaaaccgc cagggcgctg cacgctgcaa
     4621 gtattctttt catcgatggc tcccaaacga tagtgtgttt tgcattccgg cccttgtaga
     4681 ttgatcgccc gggttggcaa tgattttttc ttggcgcgga aagtacgcgc gtgcgttgcg
     4741 cgaccgtggc ggatcgggac gatccctgcg ctgaggcgcg caggcgcgga tcatccccgc
     4801 cgggccgttt tgccggcgag cgaccgcatg gaggcggcgc gccggttgcc gccggatgat
     4861 gggacgatgg ctcaggcctc gggcccgcga atcgcgaaat gctccacgcg gttgcggccg
     4921 cccttcttcg cggcgtagag cgccttgtcg gcttgctcgg tgagctcggc ctgggtgcgc
     4981 gccgtcgcgg gaaagcacga cacgccgaga ctcacggaaa gatgcaggtc cggcccctcg
     5041 agctccttca ggaaatccag ccgctccacc gcgcgacgca cgtcctcggt gaccgccatc
     5101 gcggtctcga ggtccgtgtt cggcagcagg atggtgaact cgtcgccgcc ataccgtgag
     5161 acgatatcgg ttttccggaa tgtggtggtg aacaccttcg cgacctcgac gatgtaccgg
     5221 tcgcccacct cgtggctgta gccctcgttc acctcgcgga agaagtccat gtcggccatc
     5281 acgagcgaca gcggcgcgcc ctccttctgg gccatcgtga agcgtttctc gagctcgctc
     5341 tcgaggtagc ggcggttgaa cacgccggtg agcttgtcgg tgacggcgcg caggctggcg
     5401 tcctcgcccc acttcaccat ctgcgtgatg aactgaccgc tcgcattgag ccggtcggtg
     5461 gtcttgttga gcatgctctt catcacattg atggcggtgt gtggataggt gcgcatcagc
     5521 gtgaagaagt cctccttgtt catgcggagg atctcgcagt cctccagcgc cacgcagttg
     5581 gccgagcgcg gcgcgttctc gaagatggcc atctccccga agaactcgcc gctcttgagt
     5641 tgcgtaagct ccttctcggt gccgtcctgg acgctgatgc tgaccgaggt gaccccggat
     5701 ttgatgatga acagctcgtc cccgggatca agctgccggc agatgaaggc ccccgcgggg
     5761 aagcggagca gctcgaggtg ctgcgagaac tggtggagct ccgacatctt gaggctggag
     5821 agcatgccca ccgtcttcag gaagttgtag agctcgtcct ccacggcgct cgcgacgcgc
     5881 acgccgtttc cgcccttctc gcgctgctcg aacaccttgg tgaaggcctt ttcgaccagc
     5941 tccaaaaacg cgcgctcgtg ctccggataa gtggccacgc cgatgctgaa ggtctggaac
     6001 aggctctcgc gcttcatggc gatgccgatg tcgaacgccg tcatctcgcg cagaaagaca
     6061 cccgcgatct tctcggcctc gtccagcggg gtcgatggaa acacgacgat gaactcgttg
     6121 ccccggtagc gcacggcgaa gtcggcgtcg cgcaggaggc tttgcagctt ggacgagatg
     6181 gcctgcagcg cgccgtcgcc ggcctcgtgc ccgaaggtgt cgttgatgag cttgaagttg
     6241 tccggcttga cgaccaggag gctgaagacc ggccccagaa atgcgaaatt gcgcgcgagc
     6301 tcgtcctcga ggtaggtgcg gttgtacacg ccggtgagct tgtcgaagaa catctgcttc
     6361 ttgagctcct cgatccagcc ggtcttctcg ctgatgagac ggtgcgtgag gcgctggcgc
     6421 gacgagttga ccgtgatgag cgtgtgatag atcttcacga agatgtgcgc gtactcgtcg
     6481 aagagatggt cgaggttcac gccctcgcgc gggaagacca ggatggtcgt gtcctccgca
     6541 gccgaagcga aggccgtgcg cgtgtcgccc tcgaagaact cgaactcgcc gaagatctcg
     6601 ccgccgatga agcgcgcgat ggcctgatcg ccggacggcg cctgccccag ccggtccgtg
     6661 tcgagggtgc tgttgatggc cacctccccg cggtcgatga tgaacagctg cttgctcttg
     6721 ctgcccttct cgaagatgac ctcgcccttg cggtagtcga caaaccccgc gtactgcgcc
     6781 atcacctgga ggtcgtcgtc gggcagctgc gagaagatcg ccgtcttttt caggtgcgca
     6841 attattcggt cactggtcgt ttcggtcatt gctggcatgg ctcactcttg gtcgctgttt
     6901 ctgttttgga tcgatgcgcc gccggtcgcg gcgcatcatc ataccgcttt gcatggaaag
     6961 atcgtacggc ggcggcggta acgcaagtaa aaaacggaag cattattccg gatgggtccg
     7021 gcgccactgc ccgtaccggc agagcgcccc ggccgcgcgg gccgcgcgtt ccggcgtggg
     7081 caggaacgga acgccgctct cgacgagatg cccgatgagc gattcgtcca gacccaggaa
     7141 ggtggagcag atgatcggga ccggcaggtt ccgcagcgac gcggcgatcg tgtgctggag
     7201 gtccgtgccg aggcgcagcg cggcttccat gagcgcggga ttgaggctgg gcgccacggc
     7261 gggatggtcg cgatagtgca ggaacgtctc gatgccgaag aacccgtaga agagcagcac
     7321 gtccacctcg cccgactcgg cgatgaggcg cggcacccgg ctgaagaacg cctcccagtc
     7381 gttgatgaag gtgaagtcga ccgggttcgc gagcgacgcc gtccccggcg caaaggcgcc
     7441 gatctccttt tggagcgccg gagaaaagct cgggagcgaa agccccaggc gcgacacggc
     7501 gtcggccatg agcacgccgg gcccgccgct gttggtgagg atgcccacgc ggtttcccct
     7561 cggcacggcg cagcgggaga gcacgcaggc gaaatcgaac acctcctcga tcgtcgatgc
     7621 gcgcacgatc cccgtctggc ggaagaggcc gtcgatgatc ccctcgggcg cggccatggc
     7681 ggcggtgtgc gaggccgtgg cgcgggcgcc cgcgtcggtg ccgccggcat agagcgcgac
     7741 gatcggcttg acgcgcgaga catcggccgc cgtcgccatg aactcccgac cgcgccggat
     7801 cgcctcgatg tagcaggtga tcacccgcac ctgatcgcgc gaggcgtaat actgaagccc
     7861 gtccacgatg tccaggtcgc cctcattgcc caggctgatg gcctcggcgt agttgagccg
     7921 gcgcgcgtgg agatacggca tcaggtgcgt gacgaaggtc ccggagtgcg acagcatgcc
     7981 gatgctgccg ggctcccccg caaagggaaa gaccgtgaaa ttaaggcgtg catgcagatt
     8041 ggccacgccg atgcagttgg gacccacgta gcgaagaccg gcgcggtgcg cggcctcgtt
     8101 caccgcgtcc tgcagatcgg cgccgcccac ctccgcgaac ccggcggtca cgatcacggc
     8161 gcgccggacc cccgccgcgc ccagctcgtc gatcacgccc ggaacgagcc gtgcgggcag
     8221 cacgatcacc gcgagatcga cgggcgtccc gatctcaccg acgctccgat aggcgcgaag
     8281 cccgaagacg gtttcctccg atgggtggat cggatagatc gcgccgggat atccgttgct
     8341 gatcatcgtc accagctggc tcgatcccat cttcgagacg ttgttcgacg cgccgtagaa
     8401 ggcgatggag cgcgcgttca tgatctccgt gagcggactg tgggccatcg ttacttcctt
     8461 ttcgtatgtg atgacgggcg caccgcgcgc cgcaccgcgt cgagcgtccg ccgcgcggcg
     8521 cctttctttt tcttcaggcc ctcgagatag ccggggataa aggcctcgag ctcgtcgatg
     8581 ccggtgaagt agcgctcata ggtggcgatg acgtcctgca gtccctcgca gaggaccgcc
     8641 ggctccatct ccacgccgtg ctcgcggcag atcgccgggc gcgtctcgta gatgcggcag
     8701 ga
//
LOCUS       DAORHF010000159         8700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7564_length_8700_cov_6.256564, whole genome shotgun sequence.
ACCESSION   DAORHF010000159 DAORHF010000000
VERSION     DAORHF010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7564_length_8700_cov_6.256564"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PKJ16_12670"
     CDS             complement(<1..661)
                     /locus_tag="PKJ16_12670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47888.1"
                     /translation="MHTDTKTPSPGRDNDPEKCTRDELVRLVREQRQALDALNGELDG
                     LRMITENVSDNIWILDLATLRFSYTTPSSVNILGYGPDEVDALTLERIITPRSLADAM
                     QLLQDELARDGRPGVDPDRVLTHLFEQIHRDGSIRWVDIHLRFLRDAAGAPRAIMGVS
                     RDVTERRLAEQALRESEERFRLLAEKNPTGIVVHTEGRIVYVNDFTVKSFGAASADEL
                     IG"
     gene            816..1211
                     /locus_tag="PKJ16_12675"
     CDS             816..1211
                     /locus_tag="PKJ16_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47889.1"
                     /translation="MLYELKNIAQKETGVFRRWFEDNYFELIVWYYHDRSVKGFQLCY
                     DRFRDEHAITWFDGKDVEHNRVDDERKQGSYAPTAILVADGVFPAAAVISRFREASAQ
                     IDPEIAGLVLRVLADHARGSRPKRRERAR"
     gene            1208..1990
                     /gene="cbiR"
                     /locus_tag="PKJ16_12680"
     CDS             1208..1990
                     /gene="cbiR"
                     /locus_tag="PKJ16_12680"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HNV47890.1"
                     /translation="MSTRYGTPFRIGTTSYIYPDDILPNARRLCGRVDDIELVLFEVP
                     DAGNIPGSALVRELASLARDHALTYTPHLPLDINLGSVDAVVRDSSIDIAARLIERLA
                     PLDPPAFVLHIPGPGNSCAGILDWQLRAGDSVGRLLRRVGIAPNRIAVENLDFPFHLL
                     GGIIADHALSACIDVGHLFIGGLDVSAHLDSYLGVARVMHLHGVRDGRDHISLRHLDP
                     AFTAWLMKRLCDAGYGGVLTLEVFGETDFEESLAALREALPG"
     gene            complement(2042..3661)
                     /locus_tag="PKJ16_12685"
     CDS             complement(2042..3661)
                     /locus_tag="PKJ16_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV47891.1"
                     /translation="MAAKPIIMHIAQFFKRMQRFLLWERDAFPDTYAGELNYQCGRII
                     MPASLICIVAWLSYVDVDRQLFPQEPLIVWLRYGLTAVSLVVFLLQFIPFCKRHSMLL
                     LAALGFYLEAATGVITGLSKADPVYLGGYLFVLMIPLVAPVGRVLLWGMIALSLALFF
                     TVGLSRGMEFSTLRDQYKLNDLIVTFVFSLVFIYILDRLRKASWRKSMQIEQQRARIQ
                     FDKERVESVVAESRNVAAHVRRAAAILGDFSSEVTGIIDTQSALFSRSREIGARLIES
                     FRVLTEETGREIESSRVIGDLTKRLRGDLQTTAEAGRDASADAQKIEALSDDCTRKLQ
                     DARGTIERLRDESKMIEEISQTINEISDQTNLLSLNASIESARAGEHGRGFAVVADEI
                     SKLADKSQSSAKEISGIIGRSVERILSAAVQIDDASRALADIIGFLEANRGFLLRFQE
                     LVRAQDGDFQTVIGHLENSLRYTESITGLTARNTEAVASSQEALERIEGFYAELTRMA
                     NTLRQLSGDLAGHVGNLHETLDETLKPDGAP"
     gene            3757..8073
                     /locus_tag="PKJ16_12690"
     CDS             3757..8073
                     /locus_tag="PKJ16_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47892.1"
                     /translation="MKSSETPRHHASVPPAGGAAVDRRHALRRVGRAILAIALAIAIT
                     TPLAAKEPPHRSWDDLDDDAEKIFGSEKERKYPIHAFIVEKEQWEGHSSCMIGWLYKH
                     TDYPKYRSRRVLPFYYRLESKIDNRTRTVLPLLLYYNRVDGPTERTVTPLFYSDLSDI
                     DADRSLLYLFWWGWERVPEKTRDSSYSALIPLYHYRTYETPGTPNHGWRLITPLLYLR
                     HAASQTGAETRTAGFQVSPLHWYEYRANKTTGTETTTWWAPLIPLTYHHVSPTRGHRN
                     ILWLLDYSWRVKDGESSLTRFWIMPLWFYEPGEHGYNAVLPPIYVENRHGGRGLYRHF
                     LPFFIYNREKTTQRNAKEKRVVETHDTYLVTPLYAEFDLYRTEPDGVKTPLASTVWFP
                     IIPLYYSSFDHTEGTHRNVLWLYDTHTTVAGETDRRWLAPLFFWKKDSYFHVLPPLYM
                     SWEEKNEKTWFIVNTYYSSERVRSDVRAKTTLAKKDAHPEAAQSFERHTGTLLVLPLF
                     YRGWSEYNAAGEATDVPSHSSETNFFIPLWYYRSFDSTQGGAQRGWARMEERTLVTPL
                     WYYSREFKKSDAPDHPDENESRLWVPLLPILYSHRTDREGSNRTVLVLWDWKTDPKGE
                     LTRFWFIPFYFHEYKDVGGYLYVPPFYFRPDGWTEQRGYDFGTFFYRSRSPEEELKWT
                     WLIHYYRSNPGAKDYVNVWFPFYLNWEYADWKAMSVLGPIYMDYEGTEHAWTLFLPVY
                     FNYAAKDGKRKIHINIAGLSKSVTSGANPNVSLGFGKHTRKAGWYIDTEWSWLYDVVS
                     FSTRVTVGAGEKDAAGAKQSEPPAAGKDARQKPSDAPDDATHAHPKPPAPVHKDIVIE
                     NKDRSKPVDKPKEGAVALERKKSIDREDSEFFFGWRMLFGMVAYQRADTRRHFRLLPL
                     SWITWDEKSKDEITWILLYLSYQSEESSYRVVFPFYGSQHEGKSYKQGFLLNLFWREY
                     DAGEDLKEYTVLWPFINWYASPKKRGWRFAPFIWHTSAEDGGERITRTVSPLYYGMDR
                     VRLADGRTVEDFSVSPLHYYNRDDDGTRTERQMFFPIVPLFYHATSTSAPQNSETTAW
                     IFPFFYYNREERPTDAGTARAIMHCSPLHFRSYEESPGSHESTWWFPIIPLVYRSASP
                     GFIHYNVLGIVDYERDEKNEYSRTWLLPLYYNSRSGDEKTSFILGYYSHSKGTTYSKN
                     FLYAFGYYDYPERDAWGWHALFTTVSYDVAPEVKEFRLFWGALASYTAHQNGDYAFDF
                     LWVLASLKREGDRFQSSLMPFWYYSGNPQGYTFVSPALLTYWSDHGDEKFQLVALGAL
                     WYRNAHLDRGTDRQMLLLGAPYYFIQRQERGYEERGSLWGILWQHEHERETGYSKFSL
                     LKFLFKRVDMNGDIRYRVFGIELSGGKGQSRATEPAPVPTPPPAPIGVDVEQADPAPV
                     VL"
     gene            8169..>8700
                     /gene="trpS"
                     /locus_tag="PKJ16_12695"
     CDS             8169..>8700
                     /gene="trpS"
                     /locus_tag="PKJ16_12695"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HNV47893.1"
                     /translation="MARILSGIQPSGTLHIGNYFAMMRPMIRFQAEHELFCFIVNYHA
                     LTTVDDGGALRANTLEAAMDFLALGLDPERAHFWVQGDVPEVAELTWILACQTSLGLL
                     ERSHSYKDKIAKGITPSCGLFTYPVLMAADILLYQANIIPVGRDQKQHVEITRDIAER
                     FNNVYGETFTLPEPLIN"
BASE COUNT         1605 a         2917 c         2664 g         1514 t
ORIGIN      
        1 gaccgatgag ctcgtcggcg ctcgccgcgc cgaaactctt cacggtaaaa tcattcacat
       61 agacgatccg cccctccgtg tgcacgacga tgccggtggg gttcttttcg gccagcaggc
      121 ggaaccgctc ctcgctctcc cgcagcgcct gctcggcgag ccgccgttcg gtcacgtcgc
      181 gcgagacgcc catgatggcc cttggcgccc cggctgcgtc gcgcaggaag cggagatgga
      241 tatccaccca gcggatggag ccgtcgcgat ggatctgctc gaatagatgg gtgagcaccc
      301 ggtcggggtc gacgcccggc cggccgtccc gggcgagctc gtcctgaagc aactgcatcg
      361 cgtcggcgag cgaccgcggc gtgatgatcc gctcgagcgt gagcgcgtcc acctcgtcgg
      421 ggccatagcc gaggatgttt acgctggacg gggtcgtgta cgagaagcgc agcgtcgcga
      481 ggtccaggat ccagatattg tcgctcacgt tctccgtaat catgcgcagc ccgtcgagct
      541 ccccgttcag ggcgtcgagg gcctgccgct gttcacgaac gaggcgaacg agctcgtcgc
      601 gcgtacactt ctccggatcg ttatcgcgac ccggggacgg ggtcttggta tcggtgtgca
      661 tctgacgcta ttccgcttgg aattctctgt gtcgataatc atgcccgatt tttaggatta
      721 atggcaagca cattttccct gctcgccgcg ccgctttttt cttgactgat gatcgcgccg
      781 gccataggca ctcagttgac gaacgccata tcgccatgct ctacgaactg aaaaacatcg
      841 cccagaagga aaccggggtg ttccgccgat ggttcgagga caattacttc gagctcatcg
      901 tctggtatta tcacgaccgc agcgtcaagg gcttccagct ctgctacgac cggttccgcg
      961 acgagcacgc catcacctgg ttcgacggca aagacgtgga gcacaaccgc gtggacgacg
     1021 agcgcaagca gggctcctac gcgcccacgg cgatcctggt ggccgacggc gtgttcccgg
     1081 ccgccgccgt catcagccgg tttcgcgagg cgagcgccca gatcgacccc gagatcgccg
     1141 gactcgtcct gcgcgtgctt gccgaccatg cccggggctc ccggccgaag cggcgcgagc
     1201 gcgctcgatg agcacccgct atggcacgcc ctttcggatc gggaccacct cctacatcta
     1261 tcccgacgac atcctcccca atgcgcgccg gctctgcggg cgcgtggacg acatcgagct
     1321 cgtgctcttc gaggtccccg atgcgggcaa catccccggg agcgcgctcg tgcgcgagct
     1381 cgcttcgctc gcacgcgatc acgccctcac ctacacgccg caccttccgc tggacatcaa
     1441 cctgggcagc gtcgatgccg ttgtgcgcga ttcgtcgatc gacatcgcgg cgcgcctcat
     1501 cgagcgtctc gcgccgctcg atccgccggc cttcgtgctc cacatcccgg ggcccggcaa
     1561 ctcctgcgcg ggcatcctgg actggcagct tcgcgcgggc gattcggtcg gccgcctcct
     1621 gcgccgcgtc gggatcgcgc ccaatcgcat cgccgtggag aacctggatt ttccctttca
     1681 tctcctgggc ggcatcatcg ccgatcacgc gctctcggcg tgcatcgacg tggggcacct
     1741 cttcattggc gggctcgatg tgagtgcgca tcttgacagc tacctgggcg tggcgcgggt
     1801 gatgcacctg cacggggttc gggacgggcg cgaccacatc tccctgcgcc atctggaccc
     1861 cgcgttcacc gcctggctca tgaaacggct ctgcgatgcg ggatacggcg gcgtgctcac
     1921 gcttgaggtg ttcggggaga cggacttcga ggaatcgctc gcggcgctgc gggaggcgct
     1981 ccccggctag ccgcacgccc gcgtgcgcgt tgcgagcgcc gcccgtggtg cgccgcggac
     2041 gttacggcgc cccgtccggc ttcaatgttt cgtccaacgt ttcgtgcaga tttcccacgt
     2101 gcccggcgag gtctccggag agctgccgca gggtgttcgc catgcgcgtg agctccgcgt
     2161 aaaagccctc gatgcgctcg agcgcctcct gcgagctcgc caccgcctcg gtgttgcggg
     2221 ccgtgaggcc ggtgatggac tccgtgtagc gcaatgaatt ctccaggtgg ccgatgaccg
     2281 tctggaagtc gccgtcctgc gcgcggacca gctcctggaa gcgcagcagg aacccgcggt
     2341 tcgcctccaa aaacccgatg atgtccgcaa gcgcccgcga ggcgtcgtcg atctggaccg
     2401 cggcgctgag gatccgctcc accgagcggc cgatgatgcc gctgatctcc ttggccgacg
     2461 actggctctt gtcggccagc ttcgagatct cgtcggccac gacggcgaag ccgcgcccgt
     2521 gctcgccggc gcgcgccgac tcgatggagg cgttgagcga cagcaggttc gtctggtcgg
     2581 atatctcgtt gatggtctgc gagatctcct cgatcatctt cgactcgtcg cgcaggcgct
     2641 cgatggttcc gcgcgcgtcc tgcagtttcc gcgtgcagtc gtccgagagc gcctcgattt
     2701 tttgcgcgtc ggcgctggcg tcgcgcccgg cctccgccgt cgtttgcagg tcgccgcgca
     2761 ggcgcttcgt gaggtcgccg atgacgcggc tgctctcgat ctcgcgcccc gtctcctccg
     2821 tgagcacgcg gaacgactcg atgagccggg cgccgatctc gcggctgcgc gagaagagcg
     2881 ccgactgggt gtcgatgatg ccggttacct cggaggagaa atcgcccagg atggcggcgg
     2941 cgcgccgcac gtgcgccgcg acgttccggg actccgcgac gacgctctcc acgcgctcct
     3001 tgtcgaactg gatccgcgcg cgctgctgtt cgatctgcat cgacttgcgc cagctcgctt
     3061 ttcgcaggcg gtccaggata tagatgaaca ccagcgaaaa cacgaaggtg acgatgagat
     3121 cgttgagctt gtactggtcg cggagcgtgg aaaactccat cccgcgcgaa aggcccacgg
     3181 taaagaaaag cgccagcgag agcgcaatca tcccccagag aagcacgcga cccaccgggg
     3241 cgaccagcgg gatcatcaac acgaagaggt agccgcccag gtacaccgga tcggccttcg
     3301 aaagcccggt gatcacgccg gtcgccgcct cgaggtagaa gcccaacgcg gcgagcagga
     3361 gcatgctgtg ccgcttgcag aacgggataa actggaggag gaacaccaca agcgagacgg
     3421 ccgtgagccc gtagcgcagc catacgatga gcggctcctg cgggaagagc tgccggtcca
     3481 catcgacata gctcagccag gcgacgatgc agatgaggga ggcgggcatg atgatacggc
     3541 cgcactggta attcagttcg ccggcatacg tgtccgggaa cgcgtcccgc tcccagagaa
     3601 ggaagcgctg catccgtttg aagaactgcg cgatgtgcat gatgataggc ttcgccgcca
     3661 taccgttttg tcaaacacta atagcgatat ccggcttgac aggcgcggaa atcgatgatg
     3721 atactgggcg catacgagcg cccagcagat atcacaatga aatcatcgga aacaccgcgc
     3781 catcatgcat ccgttccccc cgccgggggt gcggcggtcg atcggcgcca cgcgctgcgt
     3841 cgggtggggc gcgccatcct cgcgatcgcg ctcgcgatcg ccatcaccac accgcttgcc
     3901 gcaaaagagc cgccgcaccg cagctgggac gatctggacg acgatgcgga aaaaatcttc
     3961 ggcagcgaga aggaacggaa ataccccatc cacgccttca tcgtcgagaa ggagcagtgg
     4021 gaggggcatt cctcctgcat gatcggctgg ctctacaagc acaccgacta tccgaagtac
     4081 cgctcgcggc gcgtcctgcc cttttactat cgcctggaaa gcaagatcga caaccggacg
     4141 cggacggtgc ttccgctcct gctgtactac aaccgcgtgg acggccccac cgagcgcacc
     4201 gtcacgccgc tgttctactc cgacctgagc gacatcgacg ccgaccgctc gctcctgtat
     4261 cttttctggt ggggctggga gcgcgtcccc gagaaaacgc gcgactcctc gtactcggcg
     4321 ctcatcccgc tgtatcatta tcggacatac gaaacgccgg gcacgccgaa ccacggctgg
     4381 cggctcatca ccccgctgct gtacctgcgc catgccgcct cccagaccgg cgccgaaacc
     4441 cgcaccgccg gcttccaggt gtcgcccctg cactggtacg agtaccgcgc gaacaagacg
     4501 acgggaacgg agactacgac ctggtgggcg ccgctgatcc cgctgaccta ccatcacgtc
     4561 tcgccgacgc gcggtcatcg caacattttg tggctgctcg attactcctg gcgcgtgaag
     4621 gacggggaat cgtcgctcac gcgcttctgg atcatgccgt tgtggttcta cgaaccgggg
     4681 gagcacggct acaatgcggt cctgccgccc atctacgtgg agaaccgcca cggcgggcgc
     4741 gggctgtacc gtcatttcct gcccttcttc atatacaacc gggagaagac cacgcagcgg
     4801 aatgcgaagg agaaacgcgt cgtggagacg cacgacacct atctggtcac gccgctttat
     4861 gcggaattcg acctgtaccg gacggaaccc gatggagtga aaacgccgct cgcgtcgacc
     4921 gtctggtttc ccatcatacc gctgtactac tcgagcttcg atcacacgga gggaacgcac
     4981 cgcaacgtgc tgtggctcta cgacacgcac acgaccgtcg cgggtgagac cgaccggcgc
     5041 tggctcgcgc cgcttttctt ctggaagaag gactcgtatt tccacgtgct gccgccgctg
     5101 tacatgagct gggaggagaa gaacgagaag acgtggttta tcgtcaacac ctattactcc
     5161 agcgaacgcg tgcgcagcga cgttcgcgcg aaaaccacgc tcgcgaagaa agacgcacac
     5221 ccggaagcgg cgcagagctt cgaacggcac acgggcacac tcctggtcct gccgctcttt
     5281 taccgcggct ggtccgagta caatgccgcc ggcgaggcga cggacgttcc ctcgcattcc
     5341 tccgagacga actttttcat tccgctctgg tactatcgct cgttcgactc cacgcaaggc
     5401 ggcgcgcaac gcggctgggc gcgcatggag gagcggacac tcgtcacgcc gctctggtac
     5461 tactcccgtg aattcaagaa gagcgacgcg ccggatcatc ccgacgaaaa cgagagccgc
     5521 ctgtgggtgc cgctcctgcc gatcctgtac tcgcaccgca ccgaccgcga aggatcaaac
     5581 cgcaccgtgc tggtgctctg ggactggaag accgacccga agggagagct cacgcgcttc
     5641 tggttcatcc ccttctactt ccacgagtac aaggacgtcg gcggctacct gtatgtcccg
     5701 cccttctact tccgccccga cggctggacc gaacagcggg ggtacgactt cgggacgttc
     5761 ttctatcgga gccggtcgcc ggaggaggag ctcaagtgga cctggctgat ccactactac
     5821 cgctccaatc cgggcgcgaa ggactacgtg aacgtctggt tccccttcta cctaaactgg
     5881 gaatacgccg actggaaggc gatgtcggtc ctcggcccca tctacatgga ctacgaaggg
     5941 acggagcacg cgtggacgct tttcctgccc gtgtatttta actacgcggc gaaggacgga
     6001 aaacggaaga tccacatcaa catcgcgggg ctttccaaga gcgtgacctc gggggcgaac
     6061 ccgaacgtct cgctgggctt cggcaaacac acgcgcaagg ccggatggta catcgacacc
     6121 gagtggtcgt ggctttacga cgtggtgagt ttctccacgc gcgttaccgt gggcgcgggc
     6181 gagaaggacg ccgccggcgc gaagcaatcc gaaccgccgg ccgccggaaa ggacgcgcgc
     6241 caaaaaccga gcgacgcgcc cgacgatgcg acacacgcgc atcccaagcc gcccgcgccc
     6301 gtgcacaagg atatcgtcat cgagaacaag gaccgcagca agcccgtcga caagccgaag
     6361 gagggcgcgg tcgcgctcga gcgaaaaaaa agcatcgacc gcgaggactc ggagttcttc
     6421 ttcggctggc ggatgctctt cggcatggtg gcctatcagc gcgccgacac gcgacggcac
     6481 ttccgcctgc tgccgctctc gtggatcacc tgggacgaga aatccaagga cgagatcacc
     6541 tggatcctcc tgtacctgtc gtatcaatcg gaggaaagct cgtatcgcgt cgtcttcccc
     6601 ttctacggaa gccagcacga gggaaaatcg tacaagcagg gcttcttgct caatctcttc
     6661 tggcgcgaat acgacgccgg ggaggacctg aaggaataca cggtcctgtg gcccttcatc
     6721 aactggtatg cgtcgccgaa gaaacgcggc tggcggtttg cgccgttcat ctggcacacg
     6781 agcgcggaag acggcgggga gcgcataacg cgcaccgtga gcccgctcta ctacggcatg
     6841 gaccgcgtgc gcctcgcgga cggccgcacg gtggaggatt tctccgtgag cccgctccac
     6901 tactacaatc gcgacgacga cgggacgcgc acggagcgcc agatgttctt ccccatcgtg
     6961 ccgctcttct accacgcgac ctcgacgagc gcgccacaga actcggagac gaccgcatgg
     7021 atcttcccgt tcttctacta caaccgggag gagcgcccca cggacgcggg aacggcgcgc
     7081 gccatcatgc actgctcgcc cctgcacttc cgctcctacg aggaatcgcc cggctcgcat
     7141 gaatccacct ggtggttccc gatcatcccg ctcgtctatc gctcggcgtc gcccggattc
     7201 atccattaca acgtgctggg catcgtcgac tacgagcgcg acgagaagaa cgagtacagc
     7261 cgcacctggc tgttgccgct gtactacaac agccgctcgg gcgacgagaa aacgtcgttc
     7321 attctggggt actacagcca ttcgaagggc accacctaca gcaaaaactt cctctacgcc
     7381 ttcgggtatt acgactaccc ggagcgcgac gcctggggct ggcacgcgct cttcactacc
     7441 gtctcgtacg acgtggcgcc cgaggtgaag gagttccgcc tcttctgggg ggcgctggcg
     7501 agctacaccg cccaccagaa cggcgactac gccttcgatt tcctctgggt gctcgcctcg
     7561 ctcaaacgcg agggcgaccg cttccagtcg agcctcatgc ccttctggta ctactcgggg
     7621 aatccgcagg gctacacctt cgtctcgccg gcgctgctca cctactggag cgaccacggc
     7681 gacgagaagt tccagctggt ggcgctcggg gcgctctggt accgcaacgc gcacctcgac
     7741 cgggggaccg accggcagat gctcctcctg ggcgcgccgt actatttcat ccaacggcag
     7801 gagcgcggct acgaggagcg cgggagcctc tggggcatcc tgtggcagca cgagcacgag
     7861 cgcgagacgg gctactcgaa attctcgctc ctcaaattcc tttttaagcg ggtcgatatg
     7921 aacggcgaca tccgctaccg ggtgttcggc atcgagctct cgggcggaaa gggccaatcg
     7981 cgcgcaacgg agcccgcgcc cgtcccgacg cccccgcccg cgccgatcgg cgtggacgtc
     8041 gagcaggccg atcccgcgcc ggttgtcctg tagcggcagg gcgcccggat cataccgacg
     8101 aaaaaatcat tgtcaaaaaa aggcgcgccc gccaacatca ccgcaccctg atacgcgagg
     8161 aaccatccat ggcgcggata ctttccggca tacaaccctc gggcacgctg cacatcggca
     8221 actacttcgc gatgatgcgt cccatgatac gcttccaggc cgagcacgag ctgttctgct
     8281 tcatcgtgaa ttatcacgcc ctcaccaccg tggacgacgg cggcgcgctg cgcgcgaaca
     8341 ccctggaggc ggccatggac ttcctggccc tgggcctcga tcccgagcgc gcgcatttct
     8401 gggtgcaggg cgacgtcccc gaagtggcgg agctcacctg gatcctggcc tgccagacct
     8461 cgctcgggct cctggagcgg agccacagct ataaggacaa gatcgccaag ggcatcacgc
     8521 ccagttgcgg gttgttcacc tacccggtgc tcatggccgc cgacatcctc ctctatcagg
     8581 cgaatatcat cccggtgggg cgcgaccaga agcagcacgt ggagatcacg cgcgacatcg
     8641 ccgagcgctt caacaacgtc tacggcgaga ccttcacgct ccccgagccg ctgatcaacg
//
LOCUS       DAORHF010000160         8588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7716_length_8588_cov_5.971405, whole genome shotgun sequence.
ACCESSION   DAORHF010000160 DAORHF010000000
VERSION     DAORHF010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8588
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7716_length_8588_cov_5.971405"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(220..666)
                     /locus_tag="PKJ16_12700"
     CDS             complement(220..666)
                     /locus_tag="PKJ16_12700"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HNV47894.1"
                     /translation="MKHVSVFALVLVLLAGCAMVSKQLPVKEPEVSFVDATPHSFTFT
                     SLKVDIKLRVTNPNPIGLTIDRLDLVLYINDQKTVTAPFHNVTLKANGSSPLNTTVVV
                     PYTAVGMSMIGALKSTSGIRYKLVGVSYVKTPLGVVEFPVTIYKNS"
     gene            870..2693
                     /locus_tag="PKJ16_12705"
     CDS             870..2693
                     /locus_tag="PKJ16_12705"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HNV47895.1"
                     /translation="MKVLVVNCGSSSLKYELFDLNTETSLMSGAVSRIGMDGAEHRSS
                     RRGEESRGPIDAPDHRAALGPVLASLTAGPGAPIAGLGDIGAVAHRIGHGGTQYRGPV
                     VIDTAVTDEIRRLIPLMPLHHPAMLAGIESCRELLPDVPHVAVFDTAFHSAIPDEAAV
                     YGLPYEYFARGVRRFGFHGNSHEYVAMKAAEFLETPLRRLNIISCHLGNGASVSAIQR
                     GHSIDTSMGFSPLEGLIMGTRVGDIDPGIVTYLMREEGLDADALDGLFNRTGGLLGIS
                     GVSSDMREIIAAADAGDHRALLAIKAFCYRVKRYIGAYAAVLGGADVLIFTGGIGENG
                     RAERARCVQGLEKLGFAIDPLRNERCAVSADAPVCDIGARYSGAHILVIATDEELMIA
                     RQCARALDYKRSIKHDVLATDRRPVRVAVSVRHAHLSAADVEALFGPGYTLTERSRLY
                     QDSEFAAGETVNLVGPRGRVDDVRVLGPARAQTQVEISRTEEFKLGVDAPIRESGDLD
                     GSPGILLEGPAGSVQIPEGVICAMRHIHMSPDDAASYGVRDRDMVMVRIEGERELIFG
                     DVMVRVKPDYRLEMHLDTDEANAAELPPVSQGYLVRIESRA"
     gene            2690..3442
                     /gene="surE"
                     /locus_tag="PKJ16_12710"
     CDS             2690..3442
                     /gene="surE"
                     /locus_tag="PKJ16_12710"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HNV47896.1"
                     /translation="MIILLTNDDGVTAGGINALFRALSERHDVYLIAPNRELSACSSA
                     ITIRHPVTLERISDRIYASDGFPADCVNVGMNAGILPRPDLVVSGINHGPNMGSDVYF
                     SGTVAGARMGYIFGAPAIAVSLDRYGDSDRFDDVARFMLEFVAAASPGGAMEHVFLNI
                     NYPDLPRGEIAGCAYTTLGHRNYIDSYRRVSGDDREQRLQLVGNIESSGPDDCDVVAV
                     QNGFISITPLGLDCVDYAYLSRAREGAVPCPK"
     gene            3430..4128
                     /locus_tag="PKJ16_12715"
     CDS             3430..4128
                     /locus_tag="PKJ16_12715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47897.1"
                     /translation="MSEVDLTTRIRGFLKSALTNLESRRYDRALADLMAAEGLDRDNP
                     EVLYNLGVACCRSGDYRRSISYFRRVLDLPFTFVDVITVNKLLAFAQIQLGETEPAAR
                     DIANILKLSPDDVTLLNMLGYCHERAQRYREALAAYNRIIEIDRHNYNAYNSLAYIIA
                     TLNGDLNRALKFAKTALDASPENPAYLDTIGFVYMKKGNPDLARKNFKKALSKLPDSA
                     EIREHLNQLLELEG"
     gene            4320..4562
                     /locus_tag="PKJ16_12720"
     CDS             4320..4562
                     /locus_tag="PKJ16_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47898.1"
                     /translation="MEIRALGTPIHQELPSTAAHKALAGTGDGEDGAIKRHPIQSEQT
                     MLEPDEVKQLFFVLIGHGVHAVPGSGSLGAHVNTVA"
     gene            4894..5016
                     /locus_tag="PKJ16_12725"
     CDS             4894..5016
                     /locus_tag="PKJ16_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47899.1"
                     /translation="MSRELQERADRITRELEESLATITEEISRLRRTPHAARRP"
     gene            5052..5660
                     /locus_tag="PKJ16_12730"
     CDS             5052..5660
                     /locus_tag="PKJ16_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV47900.1"
                     /translation="MPAKGQRRKQQIIDTAKEMFIEKGFQSTHIGQVCKKLNIARGTV
                     YQYFGNKREILYAVLDQIEEKIDDIMDQDDLRDFLKNNPNPKTHAKFIEDRLINVLRT
                     IMDEPIVMKLIFKEVVGIDKEVVARVNKFQEYLATAVKNEVDEIIKKGIYKKTINPEI
                     AAMLLVGGVMFILFQYESKQRNVLDREIIESIISTYLTGVLK"
     gene            5743..7038
                     /locus_tag="PKJ16_12735"
     CDS             5743..7038
                     /locus_tag="PKJ16_12735"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV47901.1"
                     /translation="MGRHAVWTTVIAAAVLIAPGAPLHAQPVPSDQLVHGLLSDDAFE
                     QDTSRTYIHGAYPADLPPLLERKILSAIPDRTRRVAIRALRLYPVADQLDRWFTILKK
                     TDSYAVKRWVIDVLSGCKDKAVVMPLVNELSCPDAGVRERAAIALKTAGDDRMYPMVL
                     IMAKNQNPIYRVYAMEAMAHLYDQRFQTVPLEMLRDPNHTVRYYALRCAEMNNVPQSL
                     SAIRNLAGGDPNFRVRAKAISILGNYRDSGSLYIFTRALGDREPSVRLAAATGLYHLN
                     SRSAASALGGQLLHERDNAIKMMILSALARLGSAGSMHGLEKTLREDDSPLIRALSAH
                     VLGEARERHAVNALSAALADTDRRVRAEVCAALGAYREKRAQDELVRMSLSNDDRYVR
                     TAALYALRRIADRASVNALHERAAAEKDPVIKSIIESMR"
     gene            7059..>8588
                     /locus_tag="PKJ16_12740"
     CDS             7059..>8588
                     /locus_tag="PKJ16_12740"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HNV47902.1"
                     /translation="MKIAIGQINPVIADIAGNRDTILSAVHRAAGLGAQLVVFPEMAT
                     VGYPPMDLLENNTLIADNIALIDEIAHASEKIAVICGYVDRDPRHPAMLRNAAAYMAG
                     GRIVSRHAKTLLPAYDVFDETRYFTPASVQEPVVCDGVRIGITICEDIWNDQIPDMRV
                     TLDTRRYDADPVAALAARGIDLLVSINASPYTLGKNRYKWDMIGRIARSVGVPVLYAN
                     QVGGNDSLVFDGNSFLMDRAGAIIARARAFEEDLVVADTDAAPADPPYIEDIEEVRRA
                     LVLGLRDYVHKCGFADVIIGLSGGIDSALTAAIAAQALGPGHVTGITMPSMFSSRGSV
                     DDSYALAQNLGIRIETIAIADLFARYREELRPIFGDRPPDIAEENIQARIRGNLLMAI
                     SNKFGSLLLTTGNKSELAMGYCTLYGDMSGGLAVISDLPKTMVYALSRHINREREIIP
                     AATIEKPPSAELRENQKDEDSLPPYDILDGILERYIERRMSAAEIVTDGFAADTVRWV
                     LA"
BASE COUNT         1678 a         2840 c         2551 g         1519 t
ORIGIN      
        1 ggtcgtgacc gccatcggat caaagccggt gaaggcggtt tcctcgctga tcgggaacac
       61 cggcgtaccg ttcaccgtga tcggcgacgg cgtgcagccc ggaaagatca acgacgcgat
      121 ccacggcggc ttcctcgccg caacgggatt gacgtaaccg gttccctgcc gggtgattct
      181 tcggcgcccg gcaggcagga gcccgatcgc ttccggcggt caggagttct tgtaaatcgt
      241 tacggggaac tccacgacgc ccagcggcgt tttcacatag ctcacgccca ccagtttata
      301 gcggatcccg ctcgtgctct tcaacgcgcc gatcatcgac atccccacgg ccgtgtacgg
      361 aacgacgacc gtcgtgttga gcgggcttga gccgttcgcc ttgagcgtca cattgtgaaa
      421 gggcgcggtg acggttttct gatcgttgat atacaggacc aggtccagcc gatcgatggt
      481 gagcccgatg gggttggggt tggtcacgcg cagcttgatg tccaccttga ggctggtgaa
      541 ggtgaaactg tgcggcgtcg cgtcgacgaa cgacacctcc ggctccttca cgggaagctg
      601 cttcgaaacc atcgcgcatc cggccaacag gaccaacaca agtgcgaaaa ctgacacatg
      661 tttcatacta ccctcgcttc ggaaaaaatg ttacagacgc cgtccccgga agaggacgga
      721 aaccggaatg ctgtttgaca gaaccggcgc cgtctgagac tgtctcttcc gattacagag
      781 tgttttcgaa ttcatgagca ccgtcaagcc ttttccggcg cgccggacgg cgcccgggac
      841 ggacgctgaa tgatcgagag ggagcgcgca tgaaggttct tgtcgtcaac tgcgggagct
      901 cgtcgctcaa gtacgagctt ttcgacctga acaccgagac ctcgctgatg agcggcgctg
      961 tgagccgcat cggcatggac ggcgccgagc accgctcctc gcgccgcggg gaggagtccc
     1021 gcgggcccat cgacgcgccg gaccatcgcg cggcactggg tcccgttctc gcgtcgctca
     1081 ccgcgggccc gggagcgccg atcgccgggc ttggcgacat cggcgccgtg gcccaccgca
     1141 tcgggcacgg cgggacgcag tatcgcggcc ccgtggtcat cgacacggcc gtgaccgacg
     1201 agatccggcg gctcattccc ctgatgccgc tccatcatcc cgccatgctc gccgggatcg
     1261 agtcgtgccg ggagctcctc cccgacgtcc cgcacgtcgc ggtgttcgac accgcctttc
     1321 acagcgccat cccggacgag gccgccgtct acggcctccc gtatgaatac ttcgcacgcg
     1381 gcgtgcgccg tttcggcttc cacggcaact cgcacgaata tgtcgcgatg aaggccgccg
     1441 aattcctcga aacgcccctg cggcgtctca atatcatttc gtgccatttg ggcaacggcg
     1501 cgagcgtaag cgccattcag cggggccact ccatcgacac cagtatgggc ttctctccgc
     1561 tggagggact catcatgggc acgcgcgtgg gcgacatcga tcccggcatc gtcacctacc
     1621 ttatgcgcga ggagggcctg gacgccgatg cgctcgacgg cctcttcaat cggacgggcg
     1681 ggctcctggg aatctccggc gtgagctccg acatgcgcga gatcatcgcc gccgccgacg
     1741 ccggcgacca ccgcgcgctc ctggccatca aggccttctg ttaccgggtg aagcgataca
     1801 tcggcgccta tgcggcggtc ctgggcgggg cggacgtcct tatcttcacc ggcgggatcg
     1861 gcgagaacgg ccgggccgag cgcgcgcggt gcgtgcaggg cctggagaag ctcggcttcg
     1921 cgatcgaccc gctgcgcaat gagcgctgcg cggtgagcgc cgacgcgccg gtgtgcgaca
     1981 tcggcgcgcg ctactcgggc gcgcacatcc tggtgatcgc aaccgacgag gagctcatga
     2041 tcgccaggca gtgcgcgcgc gccctcgact acaagcgctc catcaaacac gacgtgctcg
     2101 ccaccgaccg ccgccccgtg cgcgtggccg tctcggtccg ccatgcgcat ctgagcgccg
     2161 ccgacgtcga ggcgctcttc ggccccggct acacgctcac ggaacgctcg cgcctctatc
     2221 aggacagcga attcgccgcc ggcgagaccg tgaacctcgt cggtccccgc ggccgggttg
     2281 atgacgtccg cgtgctgggt cccgcgcgcg cacaaacgca ggtggagata tcgcgcacgg
     2341 aagagttcaa gctgggcgtc gacgcgccga tccgcgaatc gggcgatctc gacggctccc
     2401 cgggcatcct cctcgagggc cccgccggca gcgtgcagat tcccgagggc gtcatctgcg
     2461 ccatgcggca catccacatg tcgcccgacg acgccgcgag ctacggcgtg cgcgaccggg
     2521 acatggtgat ggtgcgcatc gagggggagc gggagctcat cttcggcgac gtgatggtgc
     2581 gcgtgaagcc ggactaccgc ctagagatgc acctcgacac cgacgaggcc aatgccgccg
     2641 agctcccacc cgtttcgcag ggctatctgg tgcggatcga atcgcgcgca tgatcatcct
     2701 ccttacaaac gacgacggcg tgaccgcggg cgggatcaac gccctcttcc gcgcgctctc
     2761 ggagcgccat gacgtgtacc tgatcgcgcc caaccgcgag ctcagcgcct gctccagcgc
     2821 catcaccatc cgccatcccg tgacgctcga gcgcatctcg gatcgcatct acgccagcga
     2881 cggcttcccg gccgactgcg tgaatgtcgg catgaacgcg ggcatcctgc cccggccgga
     2941 cctggtcgtc tcggggatca accacgggcc caacatgggc tcggacgtgt acttctcggg
     3001 aacggtcgcg ggcgcgcgca tgggatacat cttcggcgcg ccggcgatcg ccgtctcgct
     3061 ggatcgctac ggcgattcgg accgcttcga cgatgttgcg cgcttcatgc tcgagttcgt
     3121 ggcggccgcg tcgcccggcg gcgccatgga gcacgtgttc ctgaacatca actatccgga
     3181 cctgccgcgc ggcgaaatcg cggggtgcgc ctatacgacc ctgggacacc ggaactacat
     3241 cgactcgtat cgccgggtga gcggcgatga ccgcgaacaa cgcctccagc tcgtgggaaa
     3301 catcgaatcc tcgggcccgg atgactgcga cgtggtcgcg gtgcaaaacg ggttcatctc
     3361 catcaccccg ctcgggctgg attgcgtgga ctacgcgtat ctctctcgcg cgcgcgaagg
     3421 ggcggtccca tgtccgaagt agatctcaca acgcgcatcc gcggctttct gaaaagcgcc
     3481 ctcacgaacc ttgagtcgcg tcgctacgac cgggcgcttg cggacctcat ggcggccgag
     3541 ggtctcgatc gggacaaccc ggaggtgctc tacaatctcg gggtggcatg ctgccgaagc
     3601 ggggactacc gccgatccat atcctacttc cgccgcgtgc tggaccttcc ttttaccttc
     3661 gttgatgtga tcaccgtgaa caagctcctc gccttcgcgc agatacagct gggcgagacg
     3721 gaacccgccg cccgcgacat cgcgaacatc ctcaagctct ctccggacga cgtgaccctg
     3781 ctcaacatgc tgggctactg ccatgagcgg gcgcagcgct accgcgaggc gctggcggcg
     3841 tacaatcgca tcatcgagat cgaccggcac aactacaacg cctacaattc tctggcctat
     3901 atcatcgcca ccctgaacgg agacctcaac cgcgcgctta aattcgcgaa gaccgcgctc
     3961 gacgcgagcc ccgaaaaccc cgcctatctc gataccatcg ggttcgtgta catgaaaaag
     4021 ggcaacccgg accttgcgcg gaaaaacttc aagaaggcgc tatccaaact gccggattcg
     4081 gcggagatac gcgaacattt gaaccaactg ctcgagctcg aaggctgacc cgtctcgccg
     4141 catacctctt gaaattcgca tgtattataa taagtgacca ataataaata tttgttataa
     4201 tacaatacca gatttctagt ttaaaaaaat tgtaaaaaaa cctattcttc ctcacaaaaa
     4261 aaccgaaaat ataatcagag taaaggagaa tcccagactt tcacacggag gtgattgtga
     4321 tggaaattag agcactcgga actcccatcc accaggaatt accgtccact gcggcgcata
     4381 aggcccttgc ggggaccggt gacggtgaag acggcgcgat caagcggcac ccaatacaat
     4441 ctgaacaaac catgctggag cccgatgaag tcaaacagct cttcttcgtc ctcatcggcc
     4501 atggcgtgca tgcggtcccc ggaagcggca gcctgggcgc gcacgtgaat actgtcgcat
     4561 aacgcagggc gcacccgctc cggttccggt tttccgcggc gtgcgccatg ccatcgaacg
     4621 cctgtttcat tccccctgtt ccccaacccc atcccgagaa tcacaccaca tacacatttt
     4681 gaatttttga tcatataaaa aaattgtgtt gacgaatacc gcatcgcgct tagtttgtac
     4741 taattctttg aattgtacta ttaatatttt aataagctaa atcttgtgcg gggttactta
     4801 taagaggtta agaacggtcc gctttcgatc gatacttgct acaaacggtt ttttttaatt
     4861 gcgggttttt gatttcgaaa ggaattcggt agtatgagca gggaactaca ggagcgggcc
     4921 gaccgcatca cgagagagct cgaagaatct ctcgcgacca tcaccgaaga gatcagccgc
     4981 ctgcggcgca ccccgcacgc ggcgcggcgt ccgtaatcgc gccgatacta tcgtcattcg
     5041 aggtacgaac catgccagcc aagggacaga gacgcaaaca gcagatcatc gacacggcca
     5101 aggagatgtt catcgagaag ggcttccaga gcacccatat cggccaggtc tgcaagaagc
     5161 tgaacatcgc tcgcgggacg gtctatcagt acttcggcaa caagcgcgag atcctctacg
     5221 ccgtgctcga tcagatcgag gagaagatcg acgacatcat ggaccaggac gatcttcgcg
     5281 acttcctcaa gaacaacccc aatcccaaga cgcacgcgaa gttcatcgag gaccgtctca
     5341 tcaacgtgct gcggaccatc atggacgagc ccatcgtgat gaagctcatc ttcaaggagg
     5401 tggtgggcat cgacaaggag gtcgttgcgc gggtgaacaa gttccaggag tatctcgcca
     5461 ccgccgtgaa aaacgaggtg gacgagatca tcaagaaggg catctacaag aagaccatca
     5521 accccgagat cgccgcgatg ctgctggtgg gcggggtgat gttcatcctc ttccaatacg
     5581 agagcaagca gcgcaatgtc ctggatcgtg aaatcatcga gtcgatcatc tcgacctatc
     5641 ttacgggcgt tctcaagtaa ccgtcccgtg tccgcgcgaa cgaccgataa aacccgttgc
     5701 ggcgggtttt atttttttcg gaacggttcg atccccgtcc gcatgggacg tcatgccgtt
     5761 tggaccacag tgatcgcggc ggcggtcctc atcgctcccg gtgcgccgct ccacgcacag
     5821 ccggtaccct ccgaccagct cgtacacggc ctgttgagcg acgacgcctt cgaacaggac
     5881 accagccgca cctacattca tggcgcatat cccgcggacc tgccgccgct tctcgagcgg
     5941 aagatcctct ccgcgatccc cgaccgtacg cggcgcgtcg ctatccgagc gcttcgcctc
     6001 tatcccgtgg ccgaccagct cgaccgatgg ttcacgatcc tgaaaaaaac cgattcatac
     6061 gcggtcaagc gctgggtgat cgacgtgctc agcggctgca aggacaaggc cgtggtgatg
     6121 cccttggtga acgagctctc ctgtcccgac gccggcgttc gcgagcgggc ggcgatcgcg
     6181 ctcaagacgg cgggggacga ccggatgtat cccatggtcc tcatcatggc gaaaaaccag
     6241 aacccgatct accgcgtgta cgcgatggag gcgatggccc atctctacga ccaacgattt
     6301 caaaccgtgc cgctcgagat gcttcgcgac ccgaaccaca cggtccgcta ctacgccctg
     6361 cgctgcgcgg agatgaacaa cgtcccccag tcgctctccg cgatccggaa cctggccggc
     6421 ggcgatccca acttccgggt gcgcgcgaag gccatctcga tcctcggcaa ttatcgcgac
     6481 agcggctcgc tgtatatttt cacccgcgcg ctgggcgatc gcgagccgtc ggtgcgactg
     6541 gccgctgcga ctggattgta tcatctgaat tcccgcagcg cggcttcggc gctgggcggc
     6601 cagctcctcc acgagcgcga caatgccatc aaaatgatga tcctctcggc gctggcgcgc
     6661 ctcggcagcg ccggcagcat gcatggcctc gaaaaaacgc tccgtgagga cgacagcccg
     6721 cttatccgcg ccctgtcggc ccatgtgctg ggcgaggcgc gcgaacgtca tgcggtgaac
     6781 gccctcagcg ccgccctggc cgacaccgac cgccgggtgc gcgccgaggt gtgcgcggcc
     6841 ctcggagcct atcgcgaaaa acgcgcgcag gacgagctgg tgcgcatgag tctctcgaac
     6901 gacgaccgct atgtccgcac ggccgcgctc tacgcgctca ggcggatcgc cgaccgggcg
     6961 tccgtgaacg cactccatga gcgcgcggcg gccgagaagg acccggtgat caagagcatc
     7021 atcgagtcga tgcgatagcg ccgcgagcgc cggaccgtat gaaaatcgcc atcggacaga
     7081 tcaatcccgt catcgccgac atcgccggca accgcgacac gatcctttcg gccgtgcacc
     7141 gcgccgccgg cctcggcgcg cagctcgtgg tgttccccga gatggccacg gtgggctatc
     7201 cgcccatgga ccttcttgaa aacaacacgc tcatcgcgga caacatcgcg ctcatcgacg
     7261 agatcgcgca cgcgagcgaa aaaatcgccg tgatctgcgg ctatgtcgat cgcgatcccc
     7321 gccatcccgc gatgcttcgg aacgcggccg cgtatatggc gggcggccgc atcgtctcgc
     7381 ggcacgcgaa gacccttttg cccgcctacg atgtgttcga cgaaacgcgc tacttcacgc
     7441 ccgcgagcgt ccaggaaccc gtcgtgtgtg atggcgtgcg catcggcatc acgatctgcg
     7501 aggacatctg gaacgaccag atccccgaca tgcgcgttac tctcgacacg cgccgctacg
     7561 acgccgatcc cgtcgccgcc ctcgcggcgc gcggcatcga cctcctcgtg agcatcaacg
     7621 cctcgcccta tacgctcgga aagaaccgct acaaatggga catgatcggc aggatcgccc
     7681 gctccgtggg ggtgccggtg ctctacgcca accaggtggg cggcaacgac agcctcgtct
     7741 tcgacggaaa cagctttctc atggaccgcg ccggcgcgat catcgcccgc gcgcgcgcct
     7801 tcgaggagga ccttgttgtc gcggacaccg acgccgcgcc cgcggaccct ccgtacatcg
     7861 aggacatcga ggaggtccgc cgcgcgctgg tgctgggact tcgcgactac gtgcacaagt
     7921 gcggtttcgc cgacgtcatc atcgggctct ccggcgggat cgactccgcg ctcaccgccg
     7981 ccatcgcggc gcaggcgctg ggtcccgggc acgtcacggg catcaccatg ccctcgatgt
     8041 tttcgtcccg cgggagcgtc gatgattcct acgcgcttgc gcagaacctc gggatccgca
     8101 tcgaaacgat cgccatcgcc gacctcttcg cccgctatcg cgaggagctg cgccccatct
     8161 tcggcgaccg cccccccgat atcgccgagg agaacatcca ggcgcgcatc cgcgggaacc
     8221 tcctgatggc gatatcgaac aagttcggaa gcctcctgct tacgacgggg aacaagtcgg
     8281 agctggcgat gggttactgc acgctctacg gggacatgtc cggcggtctc gccgtgatat
     8341 cggaccttcc caagacgatg gtgtacgcgc tctcgcgaca catcaaccgc gagcgggaga
     8401 tcatcccggc cgccaccatc gagaagccgc cctcggcgga gctcagggag aaccagaagg
     8461 acgaggactc gctgccgccc tacgacatcc tggacggcat cctggagcgt tatatcgaac
     8521 ggcgcatgtc ggccgcggag atcgtaacgg acggattcgc cgccgacacc gtccgctggg
     8581 tgctggcc
//
LOCUS       DAORHF010000161         8551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7784_length_8551_cov_4.721634, whole genome shotgun sequence.
ACCESSION   DAORHF010000161 DAORHF010000000
VERSION     DAORHF010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7784_length_8551_cov_4.721634"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2307
                     /locus_tag="PKJ16_12745"
     CDS             <1..2307
                     /locus_tag="PKJ16_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47903.1"
                     /translation="QRSPIPLIVPCTWTAPAVANGAGTFAIAGVGGSSIAAAVVGTNV
                     SGTSILDLRFQITFAWNVGGATGLATQLPAGRTISVTATCDSPSTVTNTSLLTYGFCN
                     RVRVFNMSQQGSGNTFIDDGRVSPYHAGNFTVTGTLVFDIPGAAAADGVEARTGNPAL
                     VGNKVLWKDTNNDGTVDINTTWTDVSGVDAVSVTGNYPYFVSPHPLGQSYWRFQVALN
                     GLAAGTYTCRNSLPFEVNRVHVSAMTITLGGGRDNAPYHWKSVNAAAVLFTVTATMNG
                     GTMQGNTTITINYNGGTSFPIVIPNGQNTASVIIPAGSVPAQGAGSTGTYQYYVTQIS
                     NPSYGLQGTGAFPVSAQIIDTNYGAAATYYTAQTVYWENTHAPNVAAAISGTTPSATS
                     ITVYWDHLTDTNTSSIHGDFYEYRVYYSENVGGPFSQWNGANDATLRDAGAAVTNNPF
                     PAPLSDPVRHFDATGRKYTVLPNLKLFTDYFIYVAVVDVFGNERVTATAGPIKTLPFS
                     IEVTITDGIVTYRNEDFPAASAPGDYAKRPLRESNARVDVSIVATSDKQPDSVKVWFT
                     NNMALNIVDYLAVPPVINSGAYAPPNYLESATAQKAGPNKWTAYLPTTSPIIKLGNSV
                     RFIVESSIGSASTFSDIDAVTDQDANNHPWAFIVETRTTFTPWPTRILNNVITNENPV
                     AYPAYYLTEDAYVTIKVYDIKGRPVATILDGAFRKGGQNIKEGGWRGTNKSNKKLGIG
                     LYYIHIRAVRSGGGGVILNKFMKVVMAK"
     gene            2507..2908
                     /locus_tag="PKJ16_12750"
     CDS             2507..2908
                     /locus_tag="PKJ16_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47904.1"
                     /translation="MNTGCRIFHDIVVEARIARELRTSLIEGRVIAGAGPRMTYGSLA
                     CGLKHFNAGMYFHHVTFEAICNSARLYSEGPQGADSVIREAVQPFPEVPINEDFFKAL
                     SAFELDGDVLSTMSGRLKRRHALRHRRWEGG"
     gene            2905..4047
                     /locus_tag="PKJ16_12755"
     CDS             2905..4047
                     /locus_tag="PKJ16_12755"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HNV47905.1"
                     /translation="MMGSDEPTHAAAPRADDAKRYQSIKLRLSVADMVLSLACMALVA
                     FSGISRTIAAFVAEMTIVPYFQFMLFVGLFGAALWAIGLPLDVYSSYVVEHRFGLSNQ
                     TPGGWIAERVKSFAVGLVIGVPVGLAFYAFLIHAGGHWWLFTGAVVFLVSMLLARVAP
                     QLIFPLFYSFTPLADGEVAQRLREMVRERGIDISGVYSFNLSRDSRKANAGFAGMGRG
                     RRIIISDTLLAEFTPDEIAAVFAHELGHYMNRHIAKNVALGGVIIFAALYLCGALYEW
                     SMAGFGVLDHHDLAALPMLFFYLALFGLVMVPLTNAVSRHYERAADRYALEATGRPAA
                     FISAMEKIARINLADTDPHPVIEVLWYSHPSIRRRIAMARGSTDSA"
     gene            complement(4031..4972)
                     /locus_tag="PKJ16_12760"
     CDS             complement(4031..4972)
                     /locus_tag="PKJ16_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47906.1"
                     /translation="MRKSATEEIFDEVVKRNSVLNDESVPDSSEFIRYLAATVAVPEQ
                     QIRQILRILTASHMIFTFEIVAEDPLRNVPNIEGYVAADLAIIRGLKTHFHRELSDLY
                     NQQFHKNLMPHQIIKEIFPVIKSINNTVLGQTANKAIMLEEFEKLLERDYSEFTAEWK
                     KQHLELELSRANLDHGKAAKAVDEAPLEEFTLDDEHPISSRAVDGGKYRDFISKTKNY
                     PLQRILNIYGIHFFIRVHLRKYQFDYLKKLVEDGQISRRADLTLLKRMLHSMKANRER
                     DPELRRHIHGMNELETTINHFIYFSEPGRARGVMRSP"
     gene            5190..5948
                     /locus_tag="PKJ16_12765"
     CDS             5190..5948
                     /locus_tag="PKJ16_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47907.1"
                     /translation="MNLKNKILIILGLCFILSVSSYFIIKKMFDNFENLLLEKCRIEA
                     LTGARMMSELMEFMVNQKVLTEADIMDTAYREIPGSNPKKFTTRYDKIFDSLIQKIED
                     EFLRDPDIDFAVLADRNGYVPTHNSKYSRAQTGDYAKDLVQSRSKRNFSSNPAIKKVI
                     EYTGPDTIRELYHRDTGETMWDIGAPVNFRGKHWGAFVLGVSLKRVNELKNQMVIMIV
                     MIMFVILSLTMLAVFAVIPRTILTSDLDGEQSHF"
     gene            complement(5974..6750)
                     /gene="folE2"
                     /locus_tag="PKJ16_12770"
     CDS             complement(5974..6750)
                     /gene="folE2"
                     /locus_tag="PKJ16_12770"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020678254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HNV47908.1"
                     /translation="MAELRDIQNEPDNRNLPINKVGVKNISYPVVVLDRAEGSQHTVA
                     RVNMYVDLPHHFRGTHMSRFVEILNRYQSGISTRNIREILSDMKRELAAETSHFEIEF
                     PYFVTKAAPVSGERAKMEYTCRLTGNSSQEHYTLEVRVPVLSLCPCSREISEYGAHNQ
                     RSIISIRIQAVEKIWIEDIIEIAEASASSDLYAILKREDEKFITETSYNNPMFVEDMV
                     RNAAERLMATRGILWFSVESENMESIHNHSAYAHIEMRMA"
     gene            complement(6794..7477)
                     /locus_tag="PKJ16_12775"
     CDS             complement(6794..7477)
                     /locus_tag="PKJ16_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47909.1"
                     /translation="MNDKIFGMMIIAAYMLISIAFFGIVAYVYLHSNAVKNSLITLLV
                     TAIAATAGTGLLLLPILVVKIVHHEFWLLALALTSYLAFLAAALLRWRYHEPWWRALL
                     PLPSMALTVMFLALLNYPKYRGAMDGFAFPPMTSLFNIALACVFPTMCATLRLSIRGP
                     RLTGALLIAMSLASFAWGIWYSHGRDGFISDNETSEGFVFLAVVGMLFASGIAHLRQA
                     RRSTVLKDH"
     gene            complement(7565..>8551)
                     /locus_tag="PKJ16_12780"
     CDS             complement(7565..>8551)
                     /locus_tag="PKJ16_12780"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV47910.1"
                     /translation="KYLISSTGTPYHYRMFRQTIITILFLALSACDRSPKSSRSESFR
                     PEEEILYCIGTAVNVRENPEMNAKIIGAMKAGESFSFQGMSAHQTEITEIESLDKPTR
                     RITDHWLRVTTINGGQGWIHGAYVGKRYFPRPRKRLANLPVTTRAWKGPGDNDIEGHS
                     PYVFPIGCSLEGLIAFMFYPTMNPACGSSDVLIVDPKTNKTVWGAHHCGLEDVWDMQS
                     TEINRKLNKFGIIPANFTLEKGPLETPLCRIVFSITPRTHDSMVSGYDLRRESGPEKS
                     VIAKINFGEHRAEGVLLAGWIRNPCTGKIIVMVIRVSVGETFDFIETSPYCD"
BASE COUNT         1782 a         2339 c         2512 g         1918 t
ORIGIN      
        1 caacgttcgc cgatcccatt gatcgtgccg tgtacatgga cggcgccggc cgtcgctaat
       61 ggagcgggca cctttgcgat agctggtgtc gggggatcgt cgatcgctgc agcagttgtt
      121 ggaaccaatg tgagcggcac ttcgatcctc gatttacggt tccagatcac attcgcctgg
      181 aatgtcggcg gcgctacagg gcttgcgaca caattgccag caggacgaac catttccgtc
      241 actgcgacct gtgactcccc aagcacggtt acgaacacct cgctactcac ctatggcttt
      301 tgcaatcgcg tgcgggtatt caacatgtcc cagcaaggtt cggggaacac ttttatcgat
      361 gacggcaggg tgagtcccta tcatgcgggt aattttaccg taacgggaac cctggtgttc
      421 gatattcccg gagctgccgc agcagatggt gtcgaagcca gaacaggaaa tcccgcactt
      481 gtcggaaaca aggtgttgtg gaaggatacg aacaatgacg gaacggttga tatcaacacg
      541 acctggactg atgtcagcgg tgttgatgca gtatctgtaa ctggtaacta tccctatttc
      601 gtctcaccac atccgcttgg acaatcgtat tggcgtttcc aagtagccct taatggcctc
      661 gctgcaggca cctatacctg ccggaattcg ttgccgttcg aggtgaaccg cgttcatgtg
      721 agcgcaatga cgattacact cggcggcggt cgcgataatg cgccctatca ctggaaaagc
      781 gttaatgcgg cggctgttct gttcacggta accgcgacga tgaacggtgg gacgatgcag
      841 gggaacacaa cgataacaat caattataac ggcggtacgt cgttcccaat cgtcataccc
      901 aatgggcaaa acaccgcgag cgtgattata ccggctggga gcgttccggc gcagggagcg
      961 ggtagtaccg ggacctatca gtattacgtg actcagatat ccaacccgag ctatggactt
     1021 cagggcactg gcgcttttcc tgtttcggca cagattatcg acaccaacta tggtgccgcg
     1081 gcaacatatt atacggcaca gaccgtgtac tgggagaata ctcacgcgcc gaatgtggct
     1141 gcggcgatca gcggaaccac gccatcagcg acatcgatca cggtctattg ggatcatctg
     1201 actgatacga atacgtcatc gatacatggc gacttttacg agtaccgggt gtattacagt
     1261 gaaaatgtcg gtgggccgtt ttcccaatgg aatggcgcaa acgatgcgac attgcgcgat
     1321 gctggggctg ccgtcaccaa taatccattt ccggcaccgt tgagcgatcc ggtccgtcat
     1381 tttgacgcga ccggtaggaa gtatacagta ttgccaaatt tgaagctttt tactgactat
     1441 tttatctatg tggctgtagt tgacgtcttt gggaatgagc gagtcacggc gaccgccggc
     1501 cccatcaaga cgctgccttt ctcaattgag gttacaatta cagacggcat agtcacctat
     1561 cgcaacgagg attttcctgc agcttcagct ccgggcgact atgccaagcg tccgctgcgg
     1621 gaatcgaatg ctcgcgtgga tgtttcgatt gtcgcgacta gcgacaaaca gcccgattcc
     1681 gttaaggtgt ggtttactaa caatatggcg ctgaatattg tcgattacct tgccgtgcca
     1741 ccagtcatca atagcggcgc ttatgctccg ccaaactatc tcgaatctgc tacagcacaa
     1801 aaggctggac cgaacaaatg gaccgcctat ctgccgacga catcgccaat catcaagctc
     1861 ggtaattctg tccggttcat tgttgaatca tcgatcggat cggcgtcaac tttctccgat
     1921 atcgacgcgg tgaccgatca ggacgcgaac aatcacccct gggccttcat cgttgagaca
     1981 cggaccacct tcaccccctg gcccacgcgc atcctgaaca acgtgattac aaacgaaaac
     2041 ccggtcgcct acccggcgta ctacctcacc gaggacgcct acgtgaccat caaggtgtac
     2101 gacatcaagg gccgcccggt cgcgaccatc ctcgacggtg ccttccgaaa gggggggcag
     2161 aatatcaagg agggcggctg gcggggcacc aataaatcga acaagaagct ggggatcggg
     2221 ctgtactata ttcacatccg ggccgtccgg tcgggcggcg gtggggtgat cctcaacaag
     2281 tttatgaagg tcgtaatggc gaagtaaaat tttttctccg tccatgcaaa ttttttgtca
     2341 tttttcccct ttcaacgaca ttaatctaat gagggtgtgt ttctggagat gtatcatcat
     2401 ccttgtaaaa tagagatacg tataatcaag gtccgggggg tcatggtgat ggacaccggt
     2461 agtcctgaag actatcggca ataacgctat gtgcccggag aaggccatga acaccggatg
     2521 caggatattt catgatattg ttgtggaagc tcgaattgcg cgcgaactgc ggacgtcgct
     2581 tatcgaggga cgtgtgattg ccggggccgg accgcgaatg acctatggct cgctcgcctg
     2641 cggcctgaag cactttaatg ccggaatgta ttttcatcat gtcacgttcg aggccatctg
     2701 caacagcgcc cgtctgtatt cagagggacc acagggggca gacagtgtga tccgcgaggc
     2761 ggtgcagccc tttccggagg tcccgataaa cgaggatttc ttcaaagcgc tctccgcatt
     2821 cgagctcgac ggagacgtgc tatcgaccat gtccgggcgg ctgaaacggc gacacgcgct
     2881 ccggcacaga cggtgggagg ggggatgatg ggatctgacg aaccgacaca cgcggctgcg
     2941 ccaagggcgg acgacgcgaa acgctatcaa tcgatcaagc tgcgactgag cgtggccgac
     3001 atggtcctca gcctcgcgtg catggccctg gtcgccttta gcggcatttc ccggacgatc
     3061 gccgcgttcg tggccgagat gaccatcgta ccgtatttcc aattcatgct gttcgtcggc
     3121 ctctttggcg cggcgctgtg ggcgatcggc ctgccgctgg atgtctactc gagctatgtc
     3181 gtcgagcacc ggttcgggtt atcgaaccag acgcccggcg gctggatcgc cgagcgcgtg
     3241 aagtcgttcg cggtggggct ggtgatcggc gtgccggtag ggctggcctt ctatgcgttt
     3301 ttgatccatg cgggcgggca ctggtggctt tttactgggg cggtcgtctt cctggtgtcc
     3361 atgctcctgg cgcgcgtcgc gccgcagctc attttcccgc tcttctactc atttacgccg
     3421 cttgccgacg gcgaggtcgc gcagaggctt cgcgagatgg tgcgcgagcg ggggatcgat
     3481 atttcggggg tctactcctt caatctcagt cgcgacagcc ggaaggccaa tgccgggttc
     3541 gcggggatgg ggcggggccg ccgcatcatt ataagcgata cgctcctggc ggaattcacc
     3601 ccggatgaga tcgccgcggt tttcgctcac gagctggggc attacatgaa tcgccacatc
     3661 gcgaaaaacg tcgcccttgg cggcgtcatc atcttcgctg cgctctacct ctgcggcgcc
     3721 ctctatgaat ggagcatggc cggattcggc gttctcgatc atcacgatct ggcggcgttg
     3781 cccatgcttt ttttctatct cgcccttttc gggctcgtca tggtgccgct gaccaacgcc
     3841 gtctcgcgcc attacgagcg cgcggccgac cgctacgcgc tcgaggcgac cgggcggcct
     3901 gcggcattca tttcggcgat ggagaagatc gcccggatca accttgccga caccgatccc
     3961 catccggtca ttgaggttct gtggtacagc cacccgtcca ttcggaggcg tatcgccatg
     4021 gcgcgcggtt ctacggactc cgcataacgc cgcgggcgcg gccgggttcg gaaaaataaa
     4081 tgaagtggtt gatggtcgtc tcgagttcgt tcatgccgtg gatgtggcgg cggagctcgg
     4141 gatcgcgctc gcggttggcc ttcatggagt gcagcatcct cttgagcagc gtgaggtccg
     4201 cgcgccgcga gatctgcccg tcctcgacga gctttttcag gtaatcgaac tggtactttc
     4261 gcaggtgcac gcggatgaaa aagtgaatac cgtagatgtt caggatgcgc tggagcggat
     4321 agttcttggt ctttgagatg aagtcgcggt atttgccgcc gtccaccgcg cgcgaggaga
     4381 tggggtgctc gtcatcgagg gtgaattcct caagcggcgc ttcatcgacc gctttggccg
     4441 ctttcccatg gtccaggttg gcgcggctca gctcgagctc gaggtgctgc ttcttccact
     4501 cggcggtgaa ctcggagtag tcgcgttcga ggagcttttc gaattcctcg agcatgatcg
     4561 ccttgttggc ggtctgcccc aacacggtgt tgttgatgct cttgatgacc ggaaagatct
     4621 ccttgatgat ctgatggggc atcaggttct tgtgaaactg ctggttgtat aggtcggaca
     4681 gctcgcgatg gaagtgcgtt ttgaggcccc ggatgatggc caggtcggcc gcgacgtacc
     4741 cctcgatatt cgggacgttc cgcagcggat cctcggccac gatctcgaag gtgaagatca
     4801 tatgggaggc cgtgaggatg cggaggatct gccgaatctg ctgttccggc acggcgaccg
     4861 tcgcggcgag atatcggatg aactcgctgg aatcgggaac ggattcgtcg ttcagaacag
     4921 agttccgctt tacgacctcg tcgaatatct cttcggtggc gcttttccgc atgcgtggta
     4981 tcccgaaaac ctggtccgtc ttcgtagttc ctttaacggt acaaatatac gacataattg
     5041 acaatatatt tcccgacgat gtcgtaaaaa tcaaaaaaaa acatcgcgga tggacgctgc
     5101 ccggaaaaat aattgacaat attggcattt tgttgttttg aatatatata atgccccgcg
     5161 tgaggggacc tatctgtggg aaggacgcca tgaacctgaa aaacaaaatc ctgattatcc
     5221 tcggattgtg ctttatcctg agcgtcagct cgtattttat cattaaaaag atgttcgata
     5281 acttcgagaa cctgctcctc gagaaatgcc gcattgaggc gctcaccggc gcccggatga
     5341 tgagcgaact gatggagttc atggtgaacc agaaggtcct caccgaggcg gacatcatgg
     5401 acaccgcgta ccgggagatc ccgggatcca atcctaaaaa attcaccacg cgctacgaca
     5461 agatattcga ttcgctcatc cagaagatcg aggacgaatt cctgcgcgat cccgacatcg
     5521 atttcgcggt gcttgccgac cgaaacgggt acgtcccgac gcacaattcc aagtattcgc
     5581 gcgcgcaaac gggcgattac gcaaaagacc tggtgcaaag ccggtcgaag cgtaattttt
     5641 cgagcaatcc ggccattaag aaggttattg agtacacggg ccctgatacg atccgcgaac
     5701 tgtatcaccg ggacacggga gagacgatgt gggacatcgg ggctccggtg aatttccgcg
     5761 gcaagcactg gggggccttc gtcctgggag tgtcgctcaa gcgggtcaac gagctcaaga
     5821 accaaatggt catcatgatc gtgatgatca tgtttgtgat cttgagcctc acgatgctcg
     5881 cggtcttcgc ggtgatcccc cgcacgatcc tgacgtccga cctggacggg gagcaatcgc
     5941 acttctgacg gcgcccggcg ccgggcgcgc ggatcacgcc atgcgcatct cgatatgcgc
     6001 gtaggccgag tggttgtgga tcgactccat gttctccgat tcaaccgaga accacaggat
     6061 gccgcgcgtg gccatcagcc gctcggcggc gttgcgcacc atgtcctcga cgaacatcgg
     6121 attgttgtac gaggtctcgg tgatgaactt ctcgtcctcg cgcttgagga tcgcgtagag
     6181 gtccgatgag gcggaggcct cggcgatctc gatgatgtcc tcgatccaga tcttctccac
     6241 cgcctgtatc cgaatcgaaa tgatggagcg ctggttgtgc gcgccgtact cgctgatctc
     6301 gcgcgagcag gggcacagcg acagcaccgg cacgcgcacc tcgagcgtgt agtgctcctg
     6361 cgacgagttg ccggtgagcc ggcaggtgta ctccatcttc gcgcgctcgc ccgacaccgg
     6421 cgccgccttg gtcacgaagt aggggaactc gatttcgaag tgcgaggttt cggccgcgag
     6481 ctcgcgcttc atgtcgctca gaatctcgcg gatgttccgc gtgctgatgc cgctctggta
     6541 gcggttgagg atctccacga agcggctcat gtgggtgccg cggaaatggt gcggcaggtc
     6601 cacgtacatg ttcacccgcg ccaccgtgtg ctggctcccc tcggcgcggt ccagcaccac
     6661 caccgggtag gagatgttct tcacccccac cttgttgatg ggaaggttcc ggttgtccgg
     6721 ctcgttttgg atgtcgcgca gttctgccat gtcgataaat ccggcgtcac gctatccgac
     6781 cgtctctggc ctgtcaatgg tctttaagga cggtgctgcg ccgggcctgg cgtaggtgcg
     6841 cgatcccgga cgcgaagagc atgccaacga ccgcaagaaa aacgaaccct tcgctagtct
     6901 cgttgtcgct gatgaagccg tcccggccat gtgaatacca tatgccccat gcgaaggatg
     6961 ccagcgacat ggcgatcagc agcgcgcccg tgaggcgggg gccgcggatc gacagccgca
     7021 gggtcgcgca catcgtcggg aaaacacacg cgagcgcgat gttgaacagc gacgtcattg
     7081 gcgggaaggc aaacccgtcc atcgcacccc ggtatttcgg atagttcagg agcgcaagga
     7141 acatgacggt gagcgccatc gaggggagcg gcaggagggc ccgccaccac ggctcgtgat
     7201 accgccaacg cagcagtgca gctgcgagaa aggcgagata ggacgtcaac gccagggcga
     7261 gaagccagaa ttcatgatga acaatcttca ccaccagtat cggtagaaga agcaatcctg
     7321 tgcccgctgt tgcggctatg gcggtgacga gtagcgttat caggctattt ttcacggcgt
     7381 tcgagtgcag gtatacgtaa gcgacgatac cgaagaatgc gatggatatc agcatatacg
     7441 ctgcaatgat catcatgcca aagattttat cgttcattgg atgccttggt cgctggcgtg
     7501 cgcgcttgtt ctgaagaatg atgcgcgaat ctgcgcggta aactcacgtc aattgcgcgc
     7561 gtcctcaatc gcagtacggc gacgtctcga tgaaatcgaa cgtctcgccg actgacaccc
     7621 ggatgaccat gacgatgatc ttaccggtgc agggattcct tatccagccg gcgaggagca
     7681 cgccttcggc acggtgttct ccgaagttga ttttcgcaat cacagatttt tctgggccgc
     7741 tttcgcgccg gaggtcgtaa ccggatacca tagaatcatg cgtgcgtggt gtaatggaaa
     7801 atacgatgcg gcacagcggc gtttccagcg gacctttttc gagggtgaaa ttcgccggaa
     7861 taattccgaa cttgttcaac ttccggttaa tctcggttga ctgcatgtcc caaacgtctt
     7921 ccaggccgca atgatgcgcg ccccatacgg tcttgttcgt tttcggatca acgatgagta
     7981 cgtcgctcga gccgcaggcg ggattcatcg tgggatagaa catgaatgcg atgagcccct
     8041 cgagcgagca accgatgggg aacacataag gcgaatggcc ttcgatgtca ttgtcgccgg
     8101 ggcccttcca ggcgcgtgtg gtcacgggta ggttcgcaag tcttttgcgg ggccggggga
     8161 agtaccgttt cccgacatac gcgccatgga tccagccctg gccgccgttt atagttgtga
     8221 cgcgcagcca gtgatccgtt attcgtctgg ttggcttgtc cagcgactcg atctccgtga
     8281 tctccgtttg atgcgcactc atcccctgaa acgaaaacga ttcccccgct ttcatggcgc
     8341 cgatgatttt cgcgttcatt tccggatttt cgcgaacgtt cacagcagtg ccgatgcagt
     8401 acaggatttc ctcttcgggt cggaatgact cgctgcgtga agacttcggc gagcggtcac
     8461 aggcggaaag cgcgagaaac agtatcgtta tgatggtttg tcggaacatg cgataatggt
     8521 atggggtacc tgtcgatgaa atcaagtatt t
//
LOCUS       DAORHF010000162         8518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7839_length_8518_cov_5.075505, whole genome shotgun sequence.
ACCESSION   DAORHF010000162 DAORHF010000000
VERSION     DAORHF010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7839_length_8518_cov_5.075505"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /gene="metK"
                     /locus_tag="PKJ16_12785"
     CDS             <1..870
                     /gene="metK"
                     /locus_tag="PKJ16_12785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HNV47911.1"
                     /translation="RALLLEQPFSQGVTAGEGLFKEQGAGDQGMMFGYAVNETPELMP
                     MPIQYANMLVKKLADLRKSGEITFVRPDSKSQVTVEYVDGKAKRVEAVVISTQHAPDV
                     EHTQIYEAMVELVIKKVIPASLIDANTKIYINPTGRFVVGGPHGDTGLTGRKIIVDTY
                     GGMGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVAAGLAHRCEVQVAYAIGVAEPVS
                     VMVDTFGTGKLSEDEIAKRIRSVFDLKPAGIITTLDLLRPIYLKTAAYGHFGRELPEF
                     FWEKTDKAEALKK"
     gene            1015..1158
                     /locus_tag="PKJ16_12790"
     CDS             1015..1158
                     /locus_tag="PKJ16_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47912.1"
                     /translation="MAEYKMKNFTYRCSCGFSLNVFLDAGMPQETARCRSCNREITRE
                     LVD"
     gene            1183..2808
                     /locus_tag="PKJ16_12795"
     CDS             1183..2808
                     /locus_tag="PKJ16_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV47913.1"
                     /translation="MPVRTLVSLCCAVCLAAAIAVQARPSLVRGVFARALPTAVPAAD
                     TGTRDAAATRENGSGAVAHGPDGSETPADWHAFSEDDPEAGDGASAYGGPARPGLSID
                     MQLVYGQFENMLSTISLSQEQKDVVYRLSSSYKKSNDFGYGGEIYDNTDYFENRIGFS
                     GMLAASERLRLQLNASVENYSRGMFDNPVFRREEQDLLSATFRGQFRFTDSFEAYAAV
                     GTTGFAHRLRPVVPGDGVEASSYLNMGMAETGGELVWSALNRVRYAIEYRYYNFHDRE
                     LDNRSIVLGEVADDFNLTEHVALTAGVNGAWSEYFDSLAGPIVGVTLKGFRYASVQMQ
                     YRYHLEPFRPERLYPSQKFVMPVYDLPPGRAHRGEMKAEVRVGEAVNMKIGATADQNS
                     AFYNFRPVSGNVLRAETMPATRYGGNCEANIFLLRRLVDIAFGYEYANFVAERRVTYR
                     PDHAFDGKLSYNGAWWKVQWGNRYIDPVFIDPDRADRLDRALIGSFGAQFRLLDGLFA
                     NLRVENLYNNKYNLRDGYPEPGITVLGGLRIML"
     gene            complement(2819..3247)
                     /locus_tag="PKJ16_12800"
     CDS             complement(2819..3247)
                     /locus_tag="PKJ16_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HNV47914.1"
                     /translation="MKIRSAGRPQLTLESVAMTDIIMNMFVFFFISFSLLYTFNPQRE
                     SKIKVNLPKGVTDVKSRGEKPLVVTVTGKNEIYIGSTKVSSLALSESLKSAAPTAKQH
                     GVIVKADRQAAVDYFVKVLDAAKQAGIDKVGVSIELQPRQ"
     gene            complement(3244..3879)
                     /locus_tag="PKJ16_12805"
     CDS             complement(3244..3879)
                     /locus_tag="PKJ16_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HNV47915.1"
                     /translation="MNMLTDNVIVKGGWVMIPIILGSIIALGIAIERGILLWRIRMDL
                     ARFTDEIYALVRSGELETAGAVCARTLHPIARVFKAGLEHHGEEPSVVEKAMERQGNA
                     EVARLEKNMNYLMAIVGVEPMLGFLGTILGLIGAFMAWEVSAESVTVSQLAAGIYQAM
                     ITTASGLAVAITYYVVYSVYANRIAAITRDLNSHGEELLALITGAGKKKKR"
     gene            4183..6714
                     /gene="bamA"
                     /locus_tag="PKJ16_12810"
     CDS             4183..6714
                     /gene="bamA"
                     /locus_tag="PKJ16_12810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HNV47916.1"
                     /translation="MRKLLLIACIGTVFFVLDGAVTAPSPFEGKIVKKIEFEGLRNID
                     PDDLLDIMITEEGYPLVSADLSKDVDAIFKKGNFENVRVAASEFQDGVRLRIICKVRP
                     VVKEIVIKGNDEVHRTELAEIVLQKEDEPLRIDLVEKSVRAMKEKYDKEGLFNAVITY
                     RVERSEKDEERVNVVFVIDEGEEVKIAKIIIMGARRIPVDEIMGVLELKEDGFFSDGD
                     FKRDEYEKDKGKILALYRERGYLDAQIIEDKVEYEWENPEKQEKRAIFVTIKLSEGDR
                     YFFDRYTIAGNKVIESRVFEQQFEQTKSGAIFNDTLFQKDRQMISYSYSTMGYIFARV
                     IPKRTVEERQIDDDGVMVARKYVRIDFEITEGTQAYIENIIIKGNKKTKDKVIRRELL
                     MKEGELFNAEKMRISREKVYNLGYFKEVNFDVRPGSKEGYMNLIVDVEEQPSGTISLG
                     GGYGTTSGFSIFADVAENNLMGNGQRIGARFEYGPQRTSITLSFREPWLFDYPLALNT
                     SVFYSLYTVPGASIYGQNKPAQYEKQAIGYSVSLTYRFWYYYGIGSGWTHAFKSILNP
                     TGNSSDEIFIEQSYGDQEKRTLAFFVIRNSIDNYLNPTRGWKIELDVAFTGGTVLYGH
                     DHFIKYSPDLYVYFSPFNLPFLKTHPCVIELRANAAFLTPPMNTDALERYQPQWRNRW
                     LESEDRLYMGGPETLRGWDYYDDKFPKSWRWGLFHRILYGLEFRIPIHPQMLWFVAFF
                     DAGSLWTDEFWEANMDSYAKTDIDADLDAEKLYRIDQLTKVDVMWYSKYSWGLGFKIQ
                     IPVMPLRFWFGRRMMWVQDNKQTFKGYFKEVSDFQFQFAIGDMRF"
     gene            6751..7254
                     /locus_tag="PKJ16_12815"
     CDS             6751..7254
                     /locus_tag="PKJ16_12815"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HNV47917.1"
                     /translation="MISRFTSFLAAALIAANAGCSSEPKRVEPRDLRVRAVNLSLIFD
                     FLAAKNADAKDLRERRAKLAADDAAISSQRADEGDPKKRAELDARLKKNEGELKELGK
                     REESVKARLYAEINRSVAIVAKRMDLDYILTVGDALVYSKKEHDITDEVIREIVRLHN
                     RNAPVSR"
     gene            7272..8288
                     /gene="lpxD"
                     /locus_tag="PKJ16_12820"
     CDS             7272..8288
                     /gene="lpxD"
                     /locus_tag="PKJ16_12820"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HNV47918.1"
                     /translation="MKLSAIAETLHGTLHGSDVEIDRVGALDVECAGAIMYIQGKKAL
                     AGVLAKKPAAVVIPRNFAADAIECPYIEVDDPKLAFIALLGIFGPKPKRSAGVQPGAH
                     VDPAAAIGANATVMAGAAVMAGARVGDGSVIYPNCVVETDASIGANTVLYSGVVVRER
                     CVIGADCILHSGVVIGADGYGFYEKDGKIIKIPQIGNVVIGDRVEIGANTCIDRATVG
                     STTVGNDTKFDNLVHIAHNCVIGERCYIVAQVGMSGTVTVGDKVIIAGQVGLADHISI
                     ASGAVIMGQSGIYNDVKKAEILFGTPARPVREQHRITSALGFLPELLKRVKKIEQDIE
                     EMKK"
     gene            8285..>8518
                     /locus_tag="PKJ16_12825"
     CDS             8285..>8518
                     /locus_tag="PKJ16_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein]--UDP-N-
                     acetylglucosamine O-acyltransferase"
                     /protein_id="HNV47919.1"
                     /translation="MIHNTAIIEGNVKLGQNVTVGPFAYIHGDVTIGDGTEIGPNVHI
                     EGYVSIGQRNKIYHAAYLGAPPQDVAWKGGQSFV"
BASE COUNT         1665 a         2634 c         2726 g         1493 t
ORIGIN      
        1 cgcgccctgc tccttgaaca acccttctcc cagggcgtca ctgcgggaga ggggttgttc
       61 aaggagcagg gcgcgggcga ccaggggatg atgttcggct acgccgtcaa cgaaaccccc
      121 gagctcatgc ccatgcccat ccagtatgcg aacatgctgg tgaaaaagct tgcggacctt
      181 cgcaagtccg gcgagatcac gttcgtccgc cccgactcga agtcgcaagt gaccgtcgag
      241 tacgtcgacg gcaaggcgaa acgggtggag gcggtggtga tatcgaccca gcatgcgccc
      301 gacgtcgagc atacgcagat ctacgaggcg atggtcgagc tggtcatcaa gaaggtgatt
      361 cccgcgagcc tcatcgacgc gaacaccaag atttacatca atcccaccgg ccgtttcgtg
      421 gtcggcggac cgcacggcga caccggcctc accggccgca agatcatcgt ggacacctac
      481 ggcggaatgg gccgtcatgg cggcggcgcc ttctccggca aggacccgtc aaaggtcgac
      541 cgctcggccg cgtacatggg acgctacatc gccaagaacg tggtcgccgc ggggctggcg
      601 caccgatgcg aggtgcaggt cgcctatgcc atcggcgtcg cggagcccgt gtcggtcatg
      661 gtcgacacct tcgggacggg gaagctgtcg gaggatgaga tcgcgaagcg catccgttcc
      721 gtcttcgatc tcaagccggc cggcatcatc acgacgctgg acctcttgcg ccccatctac
      781 ctgaagaccg cggcgtacgg ccatttcggc cgcgagctgc cggaattctt ctgggagaag
      841 accgacaagg ccgaggcgct taagaaatag catcatccgt tcggcttcgc accctgtgaa
      901 accccgccca tggcggggtt tcgcgttggt gcgcgagggt attctgctcg cattgcgcct
      961 cgacgcggcc gataatgaaa atggacgata ccatcatcaa catggagaaa cggcatggct
     1021 gaatataaga tgaaaaattt cacctaccgg tgttcgtgcg gattttccct caacgtgttt
     1081 ctggacgccg gcatgccgca ggagacggcg cggtgccggt cctgcaatcg cgagatcaca
     1141 cgggagctgg tcgactaggc cgctcgattc cagcagcgac gcgtgccggt gcgtactctc
     1201 gtttccctgt gttgtgccgt ttgcctcgcg gccgccatcg ccgtgcaggc gcggccgtcg
     1261 cttgtccgcg gtgttttcgc ccgggcgctc ccaacggccg ttcccgccgc cgatacgggg
     1321 acgcgcgatg ccgcggcaac gcgtgagaac ggttccggcg ccgtggcgca tggacccgac
     1381 ggaagcgaaa cccccgccga ttggcacgcc tttagcgagg atgatccgga ggccggcgac
     1441 ggcgcatcgg cgtatggcgg tcccgcccgg ccggggttga gcatcgacat gcagctggtg
     1501 tacggccagt tcgagaacat gctctcgacg atcagcctgt cgcaggagca gaaggacgtg
     1561 gtgtaccggc tgagctcgtc gtacaagaag tcgaatgact tcgggtatgg cggcgagatc
     1621 tacgacaata cggattactt cgagaatcga atcggcttca gcgggatgct ggccgccagc
     1681 gagcgcctgc gattgcagct caacgcctcg gtggaaaact attcgcgggg catgttcgac
     1741 aatccggtgt tccggcgcga ggagcaggac ctgctctcgg cgacgttccg gggccagttc
     1801 cgcttcaccg actcattcga ggcctatgcc gccgtgggaa cgaccggttt cgcgcatcgg
     1861 ctccgcccgg tcgttccggg cgacggcgtg gaggcgagca gttatctgaa catgggaatg
     1921 gcggaaacgg gcggagagct cgtctggtcg gcgctcaatc gcgtacggta cgcgatcgag
     1981 taccgctatt acaatttcca cgatcgggag ctggacaacc gcagcatcgt gctcggcgag
     2041 gtggcggacg atttcaacct caccgagcac gtcgccctga ccgccggcgt gaacggcgcc
     2101 tggagcgaat atttcgactc cctggcgggc cccatcgtgg gggtcacgct caaggggttc
     2161 cgctacgcgt cggtgcagat gcaataccga taccacctgg agcccttcag gccggagcgg
     2221 ctgtatccca gccagaagtt cgtgatgccg gtctacgacc tgccgcccgg ccgcgcccac
     2281 cgcggggaga tgaaggccga ggtgcgcgtg ggcgaggcgg tgaacatgaa gatcggcgcc
     2341 accgccgacc agaacagcgc cttctacaac ttccgtccag tgtcgggcaa cgttcttcgc
     2401 gccgagacca tgcccgccac gcgctatggc ggcaactgcg aggcgaacat cttcctgttg
     2461 cggcggctcg tcgatatcgc cttcggctac gagtacgcga acttcgtcgc cgagcggcgc
     2521 gtcacctacc gccccgatca cgccttcgac ggcaagctca gctacaacgg cgcctggtgg
     2581 aaggtccagt gggggaaccg ctacatcgac ccggtcttca tcgatcccga ccgggcggac
     2641 cggctcgacc gcgcgctcat cggctccttc ggggcgcagt ttcggctgct cgacgggctg
     2701 ttcgcgaacc tgcgcgtgga gaacctctac aacaacaagt acaatctgcg cgacggctat
     2761 cccgagcccg ggatcacggt gctgggcggt ttgcggatca tgctgtgatg gggcggcgct
     2821 actggcgggg ttgcagctcg atggagacgc cgaccttgtc gatgcccgcc tgcttcgcgg
     2881 cgtcgagcac cttcacgaaa taatcgaccg cggcctgccg gtcggccttc acgatcacgc
     2941 cgtgctgttt cgccgtgggc gccgccgact tgagcgactc ggacagcgcg agcgaggaga
     3001 ccttggtgct gccgatgtag atctcgtttt tgccggtcac ggtcacgacc agcggcttct
     3061 cgccccggct cttcacgtcc gtgaccccct tgggcaggtt caccttgatc ttcgattcgc
     3121 gctgcgggtt gaaggtgtac agcaggctaa acgagataaa aaagaacacg aacatgttca
     3181 tgatgatgtc ggtcatcgcg accgattcga gggtgagctg cggccgtccc gcggagcgga
     3241 tcttcatcgc ttctttttct tgccggcgcc ggtgatgagg gcgaggagct cctccccgtg
     3301 gctgttgagg tcgcgcgtga tggcggcgat gcggttcgcg tacacgctgt acacgacgta
     3361 ataggtgatg gcgaccgcga ggccgctcgc ggtggtgatc atggcctggt agatccccgc
     3421 ggccagttgc gagacggtga ccgattccgc gctcacctcc caggccatga aggcgccgat
     3481 gagccccagg atggtcccca ggaaccccag catcggctcc acgcccacga tggccatgag
     3541 gtagttcatg ttcttctcga ggcgcgccac ctcggcgttg ccctggcgct ccatggcctt
     3601 ctcgacaacc gacggctcct cgccgtgatg ctccagtccc gccttgaaca cgcgcgcgat
     3661 ggggtgcagg gtgcgcgcgc agacggcgcc cgccgtttcc agctcgcccg agcgcacgag
     3721 cgcgtagatc tcgtcggtga accgggcgag gtccatgcgg atgcgccaga gcaggatgcc
     3781 gcgctcgatc gcgatcccca gcgcgatgat ggagcccagg atgatgggaa tcatcaccca
     3841 gccgcccttg acaatcacat tgtcggtgag catgttcatg gtaccgtccc gtgtgcgaga
     3901 gtgcgcgagt atggcacgat tgggttcggc gcgacggatc tgtcaattct ttttatccgc
     3961 gcatgatccg gcgcgcgccg gcggccatat tttcttgaca tgggggggcg accgcgatag
     4021 caaggccaga attctcaggg cgtcatcgat gcgggaaaag ctgtaacaaa accgtccggt
     4081 cccgcatctc actgacggtg cgatgtcgga tacgccacac cgacacagcc gatcgccggc
     4141 cagcccgttc agactgaaca gacacaaggt aaccgagtac gaatgaggaa actcctactt
     4201 atcgcctgta tcggcactgt cttcttcgtg ctcgatggcg ccgtgaccgc gccgtccccg
     4261 tttgagggaa agatcgtcaa gaagatcgag ttcgagggcc tccggaacat cgaccccgac
     4321 gacctgctcg acatcatgat caccgaggag ggctacccgc tcgtctccgc ggacctgagc
     4381 aaggacgtgg atgcgatctt caagaagggc aacttcgaga acgtgcgcgt cgcggcgagc
     4441 gagttccagg acggcgtgcg tttgcgcatc atctgcaagg tccgtcccgt ggtcaaggag
     4501 atcgtcatca agggcaacga cgaggtccac cgcaccgagc tcgccgagat cgtcctccag
     4561 aaggaggacg agccgctccg gatcgacctg gtcgagaaga gcgtccgggc gatgaaagag
     4621 aagtacgaca aggaggggct cttcaacgcg gtcatcacct accgggtgga gcgctcggag
     4681 aaggacgagg agcgcgtgaa cgtggtgttc gtgatcgacg agggcgagga ggtgaagatc
     4741 gccaagatca tcatcatggg cgcgcgccgc atccccgtcg acgagatcat gggcgtcctc
     4801 gagctcaagg aggacgggtt tttctccgac ggcgacttca agcgcgacga gtacgagaag
     4861 gacaagggga agatcctggc gctgtaccgc gagcgcggct acctcgacgc gcagatcatc
     4921 gaggacaagg tcgaatacga gtgggagaac cccgagaagc aggagaagcg ggcgatcttc
     4981 gtcaccatca agctcagcga gggggaccgc tactttttcg accggtacac catcgccggc
     5041 aacaaggtga tcgagagccg ggtgttcgag cagcagttcg agcagaccaa gagcggcgcg
     5101 atcttcaacg acacgctctt ccagaaagac cgccagatga tcagctattc ctactccacg
     5161 atgggctaca tcttcgcgcg cgtgatcccc aagcgcaccg tggaggagcg gcagatcgac
     5221 gacgacggcg tgatggtggc ccgaaaatac gtgcgcatcg acttcgagat caccgagggg
     5281 acccaggcct atatcgaaaa catcatcatc aaggggaaca agaagaccaa ggacaaggtg
     5341 atccggcggg agctcctcat gaaggagggg gagctgttca acgccgagaa gatgcggata
     5401 tcgcgcgaga aggtgtacaa tctcggatac ttcaaggagg tgaacttcga cgtccgcccg
     5461 ggcagcaagg aggggtatat gaacctcatc gtcgacgtcg aggagcagcc ctccggcacc
     5521 atatcgctgg gcggcggcta cgggaccacc tccggcttct cgatcttcgc cgacgtcgcg
     5581 gagaacaacc tcatggggaa cggccagcgc atcggggcgc gcttcgagta cggcccgcag
     5641 cgcacctcga tcacgttgag cttccgcgag ccgtggctct tcgactatcc gctggcgctg
     5701 aacacctcgg tattctattc gctctacacc gttcccggcg cgtccattta cgggcagaac
     5761 aagcccgcgc agtacgagaa gcaggcgatc ggctactcgg tgagcctcac ctatcgcttc
     5821 tggtattact atggcatcgg ctcggggtgg acgcacgcct tcaagtccat cctcaatccg
     5881 accggcaaca gctcggacga gatcttcatc gagcaatcgt acggcgacca ggagaagcgg
     5941 acgctcgcct tcttcgtcat ccgcaactcc atcgacaact atctcaatcc cacgcgcgga
     6001 tggaagatcg aactggacgt cgccttcacc gggggcaccg tcctgtacgg gcacgaccac
     6061 ttcatcaagt attctcccga cctgtacgtg tacttcagcc cgttcaatct tccgttcctg
     6121 aagacgcatc cctgcgtgat cgagcttcgc gcgaacgccg cgttccttac cccgccgatg
     6181 aacacggatg cgctggagcg ctatcagccc caatggcgaa accgctggct cgaatcggaa
     6241 gaccgcctct acatgggcgg cccggagacg ctgcgcggct gggactatta cgacgacaag
     6301 ttccccaagt cgtggcgatg gggcctgttc catcgaattc tctacgggct tgagttccgc
     6361 attcccatcc atccgcagat gctgtggttc gtcgccttct tcgacgccgg gtcgctgtgg
     6421 accgacgagt tctgggaggc gaacatggac tcgtacgcga aaaccgacat tgacgccgac
     6481 ctcgacgccg aaaagctgta ccgcatcgac cagctgacca aggtcgacgt gatgtggtat
     6541 tcgaaatatt cgtggggcct cgggttcaag atccagatcc cggtgatgcc gcttcgcttc
     6601 tggttcgggc ggcgcatgat gtgggtgcag gacaacaagc agaccttcaa gggttatttc
     6661 aaggaggtca gcgacttcca gttccagttc gcgatcgggg acatgcgatt ttagctggac
     6721 agatttaccc gcatcgcgct atgttcgacc atgatttcgc gcttcacgtc atttctcgcc
     6781 gccgcgctga tcgccgccaa cgccgggtgc tcctctgagc caaaacgcgt cgagccgcgc
     6841 gatctgcgcg tccgggcggt gaacctctcg ctcatcttcg actttctggc tgccaagaac
     6901 gccgacgcga aggaccttcg ggagcgccgc gcgaagctcg cggccgacga cgcggcgatc
     6961 tcgtcgcagc gcgccgacga gggcgatccg aagaagcgcg ccgagctcga cgcgcggctg
     7021 aagaagaacg agggcgagct caaggagctc ggcaagcggg aggagtccgt caaggcgagg
     7081 ctctacgctg agatcaaccg gtccgtcgcg atcgtcgcga agcggatgga cctggactat
     7141 atactgacgg tcggggacgc gctggtctac tcgaagaagg agcacgacat caccgacgag
     7201 gtgatccgcg agatcgtgcg cctgcacaac cggaacgccc ccgtgtcacg gtaagccaag
     7261 gaggatggcc catgaaactg tccgcgattg ccgaaacgct tcacgggacc ctgcacggat
     7321 ccgacgtcga gatcgaccgg gtgggtgcgc tcgatgtcga atgcgccggg gcgatcatgt
     7381 acatccaggg gaagaaggcg cttgccggcg tgctcgcgaa aaaacccgcc gcggtggtga
     7441 tcccgcgcaa tttcgcggcc gacgccatcg agtgtccgta tatcgaggtt gacgatccca
     7501 agctcgcctt catcgcgctc ctcgggatct tcgggccgaa accgaaacgt tccgcgggcg
     7561 tgcagcccgg cgcgcatgtc gatccggccg cggcgatcgg cgcgaacgcc acggtgatgg
     7621 ccggcgcggc ggtgatggcc ggcgcgcgcg tgggcgacgg cagcgtgatc tatccgaact
     7681 gcgtggtcga gacggacgcg tcgatcggcg cgaacacggt cctgtactcg ggcgtggtgg
     7741 tccgcgagcg ttgcgtgatc ggcgccgact gcatcctgca ttccggcgtg gtgatcggcg
     7801 ccgacggcta cgggttctac gagaaggacg gaaagatcat caagatcccg cagatcggga
     7861 acgtggtcat cggcgaccgc gtggagatcg gcgcgaacac ctgcatcgac cgcgccaccg
     7921 tgggctccac caccgtgggc aacgacacca agttcgacaa cctggtgcac atcgcgcaca
     7981 attgcgtgat cggcgagcgc tgctacatcg tggcgcaggt gggcatgtcc ggcacggtca
     8041 ccgtgggcga caaggtgatc atcgccggcc aggtggggct ggccgatcat atctctatcg
     8101 cctctggcgc ggtcatcatg gggcagagcg gcatctacaa tgatgtcaag aaggccgaga
     8161 tcctcttcgg gacgcccgcg cggcccgtgc gcgagcagca ccgcatcacc agcgcgctcg
     8221 ggttcctgcc ggagctcctg aagcgcgtga aaaaaatcga acaagacatc gaggagatga
     8281 agaaatgata cacaacacgg ccatcattga ggggaacgtg aagctgggac agaacgtcac
     8341 ggtggggccc ttcgcctaca tccacggcga cgtcaccatc ggcgacggca ccgagatcgg
     8401 ccccaacgtg cacatcgagg gctacgtgag catcggccag cggaacaaga tctatcacgc
     8461 cgcctacctc ggcgcgccgc cgcaggacgt cgcctggaag ggcgggcagt cgttcgtg
//
LOCUS       DAORHF010000163         8422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7978_length_8422_cov_5.349946, whole genome shotgun sequence.
ACCESSION   DAORHF010000163 DAORHF010000000
VERSION     DAORHF010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7978_length_8422_cov_5.349946"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="PKJ16_12830"
     CDS             complement(<1..813)
                     /locus_tag="PKJ16_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47920.1"
                     /translation="MAHAVPGRRGRGIASVRVVAPLACVCAVLLAGIAAVSQEGGNAP
                     VYNPHEDLEVRPRPGGLFPDLADRTWFKRGDREPDPRAFSDVRDDADLSSIKRGATDD
                     INNIIVLVGEIPLPNTKAAVLAPIFMTNHKNIFGTISDFNFTWVGYKVTGQFKQKGFP
                     FEKTDLSETIIGSFVHASGTNLGFIGNKYEEELRFYTNYVSEIVSMRYNFPHKLSAAF
                     TLDSRQYFFVKRDVPDDFIMPHNHVNIFPRLDFNIDWLTETGIDQITKGFGLQ"
     gene            complement(869..3943)
                     /locus_tag="PKJ16_12835"
     CDS             complement(869..3943)
                     /locus_tag="PKJ16_12835"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV47921.1"
                     /translation="MRLERTLWKVFSIQRDELRFALPLFALYLLSGSFYAIGQIITET
                     LFLKAYGAAGLSRFFIFNGIALIVGGVVYTNIVTRFSLRKGYLALILLFSSLIVAGAH
                     APLYEYSWVPFYLYLGNYLFTFFLDIHFFNYATQFCNVRSAKRILPFVMAGGKMGGIV
                     SSLLIFWRFSQDIETVGLLWWAINGFLLVGPLALLRKKNAGVLPPASRSDDDLLGGRG
                     LMGLLADKLAATYSSPIFLYSALAVFVMSVANQVSEYYFASIFNAAFTSKRDLAAFLS
                     AYTVLADGATLLLQMLVVSRLITAIGVKKSNVLYPLSFIGSIVLALSFPTLVAGVLLR
                     FYRKHLSVLVRAPVFNVIMAAAPAERLTAVKSFIGGIISPLGMIAGGGAIMLVFSRFE
                     PAIVSAIAIAIGLAYVGFTLLQNRAYVRALRDRIEHGIQADDAPEADYRDFVRFVARG
                     GRVERHLGALEGIVRTHRSFEAMRLIEPHFARLQTPTRLALVRLAATGSPEERRTILM
                     RAVADPEQSVRAAALELVRTYPYGERTPFLAAYHPDAGPIERDAVRALGTATGADPDG
                     FAARRLRELAAGVENGTARDTDLRLFVAALPPGLYLETLVRIAVATGSLVLARAVIPH
                     ADSLSPDDAKAVFRVLLNGSREDIINFCTLAERLELEDRLALLTGEIEFRPDELERVF
                     AKSDEAYRAAVAMLFEERSYECKSNCLACLIALGYKPEGEMYEYISYVMIQILEALEV
                     KNSVRTHADRAVRGVPIASFVEIALASEIALQKRLILKAISILSGTHIDEAYDASLFV
                     MDRSLNSYILEFLEAYGRHTRQALFIFEDERDLSEVVVLERSLFDTELLSAILRCSSF
                     LPRSSSLLQVCRTALLDDPHSAAIESDPEVAAMLTIMEKIIFLNDSSLFAELHVEELM
                     RLAAIAREIELPRGSAFITEGDDGDDLFIVIDGEVEVFTAEKVVATLGRGSCIGELAI
                     IDREPRVASVRTVTPARLLSVSRRDFLLTLKDNPVIAINILKVIADRLRKLL"
     gene            4180..4935
                     /locus_tag="PKJ16_12840"
     CDS             4180..4935
                     /locus_tag="PKJ16_12840"
                     /inference="COORDINATES: protein motif:HMM:NF024058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3782 domain-containing protein"
                     /protein_id="HNV47922.1"
                     /translation="MNKTTRTSITITPAVRKKIVEIIDERIRDVHVTREDFSELKSIV
                     QDLATAQKRTESRIEELASAQQRTESRIEELASAQQRTESRIEELASAQQRTESRIEE
                     LASSQTRTESRLEELAIAQKRTEHTIERGFKDLRDSISALGSRWGIMAEDTFRNAITG
                     LLEGTGYSVSRGTYGDREVDIVVKNGEHLLFEITSSMKKSDIARYVRSADDYQDRTGI
                     SPRIMVAAIYIPPTVMREIADAPRTIEIFSIEE"
     gene            complement(4964..6055)
                     /locus_tag="PKJ16_12845"
     CDS             complement(4964..6055)
                     /locus_tag="PKJ16_12845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV47923.1"
                     /translation="MRVLIIRFSSIGDIVLTTPLVAAITERHPEAVIDYLTLADYRVL
                     LEHNPHINQLIAIPRRASLAENVAGARAISRNGYDLVVDLHRSTRSAVYRRFVRAREK
                     RTLDKRYVKRALLVSAKMNLYREPLSIVHRYFHVARALGVTPTGAPAEIWDRYESLIA
                     SARVLEALLGFRFAIAAMDRAAVTPIRREIVATGAPGPVISLMPFATWRTKEWGDDRF
                     VELGRRLVSERGAIVLVHGGPGDTARAEPIVRAIGPGAVMLAGATTLMQSALALAASD
                     CLVTNDTGLMHIGGAVRIPVIAIFGSTVEELGFFPYETAGTVMQVDLSCRPCTTKGLR
                     ACPKRHFRCMNDINVDSVVDAIGRFPARR"
     gene            complement(6064..6459)
                     /gene="aroH"
                     /locus_tag="PKJ16_12850"
     CDS             complement(6064..6459)
                     /gene="aroH"
                     /locus_tag="PKJ16_12850"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNV47924.1"
                     /translation="MAVRGIRGATTVDANTREDILTRTTELLERLVALNGVELEDVAS
                     AIFSVTDDVSAEFPAVAARRLGWIYTPLFCTREIGVPGSLSSCIRVLLHVNSDKRQEE
                     MVHVYLHQARSLRPDLGNGSENRYYQSEP"
     gene            complement(6561..7862)
                     /gene="aroA"
                     /locus_tag="PKJ16_12855"
     CDS             complement(6561..7862)
                     /gene="aroA"
                     /locus_tag="PKJ16_12855"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HNV47925.1"
                     /translation="MRVTPARTNTVNYIVNPSTLSGTVAIPGSKSHTIRALVFALLAE
                     GTSVIEAPLSSSDTRSCVDMIRAFGAEVLDAGGAWTVTGMGGAPAIPDNVIDVGNSGT
                     TLYIGLAVAALCTGHTVFTGDAQIRNRPADALLRAIGDLGGSAVSTRSNGKPPVVIGG
                     PLRGGETSIEAVTSQYLTALLVAAPLVSAETIINIPLLYEVPYVRMTLGWLDRLGIRY
                     RNENLARITVPGGQRYPAFREYVPADFSSATFFLVAAAIAGTGVTLTGLDFSDTQGDK
                     EVVSVLRAMGAVIEIAERSISIAPGALAGGVFDLNAMPDSLPALAVAGCFASGETRLV
                     NVPQARLKETDRIAVMCRELRSMGADIEELPDGLIVRRSRLRGAQVHGHGDHRVVMAL
                     AVAAGAAEGTTVIDTAEAVGVTFPGFRELMCGIGADIALGE"
     gene            complement(7906..8298)
                     /locus_tag="PKJ16_12860"
     CDS             complement(7906..8298)
                     /locus_tag="PKJ16_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47926.1"
                     /translation="MMHGRILAFLIIASCVLGACGSKDELKKSVEAYWELIKTGEYEK
                     SYLLCAPAYRQGHPEDNYVRNFNALFSEEGNQIRQYRIDGMDIKNGTAIVRMRLEFSE
                     GVVEHWDDTWVRDNGTWRFNGGGKLLRK"
BASE COUNT         1754 a         2669 c         2554 g         1445 t
ORIGIN      
        1 ctgaagcccg aaccccttgg tgatctggtc tatgccggtc tcggtgagcc agtcgatatt
       61 gaaatcgagc cgcgggaaga tgttcacgtg attgtgcggc atgatgaaat cgtccggcac
      121 gtcgcgcttg acgaagaagt actggcgcga atcgagcgtg aacgcggccg agagcttgtg
      181 cggaaaatta taccgcatcg atacgatctc ggagacgtag ttcgtgtaga agcgcaactc
      241 ctcctcgtac ttgttcccga tgaagcccag gttggtgccg ctggcgtgga cgaacgaccc
      301 gatgatggtc tcggagagat cggtcttttc gaaggggaac cccttctgct tgaactgacc
      361 ggtgaccttg tatccgaccc acgtgaaatt gaaatcggaa atggtgccga atatgttctt
      421 gtgatttgtc atgaagatgg gcgcgagcac cgcggccttg gtgttgggaa gcgggatctc
      481 gcccaccagg acgatgatgt tgttgatatc gtcggtcgcg ccgcgtttga tcgacgagag
      541 atcggcgtcg tcgcggacgt cggagaacgc tcgcggatcc ggctcgcgat cgccgcgctt
      601 gaaccaggtc cgatccgcca ggtccggaaa cagcccgccc ggtcgcggcc gcacctccag
      661 gtcttcgtgc ggattgtaca ccggggcgtt cccgccctcc tgcgatacgg cggcgattcc
      721 ggcgaggagc acagcgcaca cgcacgcgag gggcgccacg acgcggacgg acgcgatgcc
      781 gcgcccgcgc cgccccggga ccgcatgggc catcagtcgc ttcaaccgct ggaacaatgg
      841 cataccctta cacaggcgga tcgggagatt agagcagctt acgtaacctg tcggcaatga
      901 ccttcagaat gttgatagcg ataacgggat tgtccttgag tgtcaacaga aaatccctgc
      961 gactgaccga caacagccgc gcgggcgtga cggtgcgcac cgaggcgacg cggggttcgc
     1021 gatcgatgat cgccagctcg ccgatgcagc tcccccgccc cagcgtcgcg accaccttct
     1081 cggccgtaaa cacctccacc tcgccgtcga tgacgatgaa caggtcgtcg ccgtcgtcgc
     1141 cctcggtgat gaaggcgctt ccgcgcggaa gctcgatctc gcgggcgatg gccgccagac
     1201 gcatgagctc ctccacgtgc agctcggcga acagactgct gtcgttgagg aagatgatct
     1261 tctccatgat ggtaagcatg gcggcaacct ccgggtctga ttcaatcgcg gcgctgtgcg
     1321 gatcatccaa gagcgccgtg cggcacacct gcaggagcga cgagctgcgc ggcagaaagg
     1381 acgagcagcg caggatcgcg ctcaacagct ccgtatcgaa gagcgagcgc tccagcacaa
     1441 cgacctcgga gaggtcccgc tcgtcctcga agatgaacag cgcctgtcgg gtgtgacgcc
     1501 cataggcctc gaggaattcg aggatgtaac tgttgagcga acggtccatg acgaagaggc
     1561 tcgcgtcgta cgcctcgtcg atatgggtgc cgctgaggat cgagatcgcc ttgaggatca
     1621 accgcttctg caatgcgatc tcgctcgcga gcgcgatctc gacgaatgag gcgatcggca
     1681 cgccccggac cgcgcgatcg gcgtgcgttc ggaccgagtt cttcacctcg agcgcctcga
     1741 ggatctgtat catcacatag cttatatact cgtacatttc cccctcgggc ttgtatccca
     1801 gggcgataag acaggcgaga cagttggact tgcattcgta gctgcgctcc tcgaagagca
     1861 ttgcgacggc ggcgcgatag gcctcgtccg atttcgcgaa cacccgctcg agctcgtccg
     1921 gccggaactc gatctcgccc gtcagcagcg cgagccggtc ctcaagctcc aatcgttcgg
     1981 cgagggtgca gaaattaatg atgtcttcac gcgacccgtt caggagaacg cggaagaccg
     2041 ccttcgcatc gtccggagaa agcgaatcgg catgcggaat gacggcccgc gccagcacca
     2101 ggcttccggt cgccacggca atgcgcacca gcgtttccag atagagcccc ggcggaagcg
     2161 ccgcgacaaa gaggcgcaga tcggtatcgc gcgcagtgcc gttttcgacg ccggcggcga
     2221 gctcccggag acggcgggcg gcgaagccgt ccggatcggc ccccgtggcc gttcccaatg
     2281 cgcgaacggc gtcgcgctca atcggaccgg catcgggatg atacgcggcg agaaagggcg
     2341 tccgctcgcc gtatggatac gtgcgcacga gctcgagcgc cgccgctcga accgattgct
     2401 ccggatccgc gacggcgcgc attaaaatcg ttcgtcgctc ctcggggctg cccgttgcgg
     2461 ccagtcgcac gagcgctagt cgggtcggcg tttgcagacg cgcgaaatgg ggctcgatga
     2521 gacgcatcgc ctcgaaggac cggtgcgtgc gcacaatacc ctcgagcgcg ccgagatggc
     2581 gctccacccg tccgccgcgc gcgacgaatc gaacaaaatc gcgatagtcc gcctccgggg
     2641 cgtcatcggc ctggatgccg tgctcgatgc ggtcgcgcag cgcgcgcaca tacgcccggt
     2701 tctgcagaag cgtaaagccc acatacgcca gaccgattgc gatggcgatc gccgagacga
     2761 tcgccggctc gaaccgagag aacaccagca tgatggcccc accccccgcg atcatcccca
     2821 gcggcgatat gataccgccg atgaacgact tcaccgcggt gagccgctcg gcgggggcgg
     2881 ccgccataat cacgttgaac accggggcgc gcaccagcac gctgaggtgc tttcgataaa
     2941 accgcaggag cacgccggcc acgagcgtcg ggaacgagag cgcgaggacg atgctcccga
     3001 tgaacgagag cgggtagagc acattcgatt ttttgacccc gatggccgta ataagccgcg
     3061 acaccacgag catctggagc agcagcgtgg ccccgtccgc gagaacggta taggcgctca
     3121 ggaaggcggc gagatcgcgc ttgctcgtaa aggcggcgtt aaaaatgctg gcgaagtagt
     3181 attccgacac ctggttggcg accgacatca cgaacaccgc aagcgccgag tacagaaaga
     3241 tcggcgatga ataggtcgcc gcgagcttgt cggccaggag ccccatgagc ccgcgtccgc
     3301 ccaggagatc gtcatcggag cgcgacgccg gcgggagaac gcccgcgttt ttcttgcgaa
     3361 gcagggcgag cggccccacc agcagaaatc cgtttatcgc ccaccagagc agcccgacgg
     3421 tctcgatatc ctgggaaaag cgccagaaga tgagcaggct cgacacgatc ccgcccattt
     3481 ttccgccggc catcacgaag ggcaggatcc gcttcgcgct gcgcacattg cagaactgcg
     3541 tcgcataatt gaaaaaatga atatcgagaa agaatgtgaa caggtagttt cccagataca
     3601 gatagaatgg cacccaggaa tactcataca gcggcgcatg ggccccggcg acgatgagcg
     3661 acgagaacag gaggatcagc gccagatatc cctttcggag cgagaagcgg gtaacgatat
     3721 tggtgtagac caccccgcct acgatcaggg cgatgccgtt gaaaatgaaa aaccgggaca
     3781 atccggccgc cccgtatgcc ttcaggaaga gcgtttcagt gatgatttgc ccgatggcat
     3841 agaaggaccc cgagaggaga tacaacgcga aaagcggaag ggcaaagcgc agctcatcac
     3901 gctggataga aaagaccttc cataacgtcc gctcaagcct catgggtgcc ggttcccgcc
     3961 tggatataaa cgcatgcgac gctgtatgtc gcaaatcgtt tatatgcaat cacattttct
     4021 gcaccatgta cgcgccaccg ccttcttcgg gcggagcgat gacaaatttt attgactttt
     4081 cccaggcgtc agtcgtaatt caaatatacg gcatgcgcgc ttgtttcggc ttgatagatg
     4141 cagcgcggga gccaacttga gatttgggaa tgctataata tgaataaaac cacacgaaca
     4201 tccataacca ttaccccggc ggtcaggaaa aagatcgtcg agataatcga cgagcgaatt
     4261 cgtgatgttc atgtgacgcg cgaggatttc tcggagctca aatcaatcgt gcaagatctc
     4321 gccaccgcgc aaaaacggac cgaatcgcgc attgaagagc tcgcaagcgc ccaacaacgg
     4381 accgaatcgc gcatcgaaga gctcgcaagc gcccaacaac ggaccgaatc gcgcatcgaa
     4441 gagctcgcca gcgcccaaca acggaccgaa tcgcgcatcg aagagctcgc cagctctcaa
     4501 acccggaccg aatcgcgcct cgaggagctc gccatcgccc agaaacgaac cgagcatacc
     4561 attgagcgcg gcttcaaaga tctcagggac agcatctccg cattggggag ccgctgggga
     4621 atcatggccg aggatacgtt ccgaaatgcc ataaccgggc tactggaagg gaccgggtat
     4681 tccgtttcgc ggggaaccta tggggaccgc gaggttgata ttgtcgtaaa gaacggggag
     4741 cacctgctct tcgagataac ctcgagcatg aaaaaaagcg acatcgcccg ctatgtgcga
     4801 tcggccgatg attaccagga tcgcaccggc atctcaccac gcatcatggt ggcggcaata
     4861 tacatcccgc ccacggtcat gcgcgagatc gcggacgccc cgcgcacgat agaaatcttt
     4921 tcgatcgagg agtgagccgg cgcgatttcg atatgccgtc cctctatcgg cgcgccggga
     4981 agcgtccgat cgcatcaacg accgaatcaa cattaatgtc gttcatgcac cggaagtgcc
     5041 gcttgggaca ggcccgaagc cctttggtgg tgcagggtcg gcaggataga tccacctgca
     5101 tgaccgtccc cgcggtctcg tagggaaaaa atccaagctc ctcaaccgtt gagccgaaga
     5161 tcgcaatgac cggaatgcgc accgcgccgc caatatgcat gaggccggtg tcattcgtca
     5221 ccagacagtc cgatgccgca agggcaagcg cgctctgcat gagggtcgtc gcaccggcga
     5281 gcatcaccgc acccggcccg atggcgcgga cgatcggctc cgcgcgcgcc gtatcgcccg
     5341 gcccgccatg aaccaggaca atcgccccgc gctccgaaac caaccggcgg ccgagctcga
     5401 caaaccggtc atcgccccac tccttggtgc gccaggtcgc gaagggcata agcgaaatca
     5461 cgggcccggg cgcgcccgtg gcaacgatct cccggcgtat cggggtcacc gcggcgcggt
     5521 ccatcgctgc gatcgcgaag cggaagccaa gcagcgcctc gagcacccgg gccgacgcga
     5581 tcagcgactc gtaacggtcc catatctccg cgggcgcgcc tgttggcgtg acgccgagcg
     5641 cgcgggcgac atgaaagtag cggtgcacga ttgaaagcgg ttcgcgatag aggttcattt
     5701 tcgcagacac cagaagcgcc cgcttgacat agcgtttgtc gagcgttcgc ttttcgcgcg
     5761 cacggacgaa acgccgatac accgcgctgc gcgtgctgcg gtgtagatcg accacgaggt
     5821 cgtatccgtt gcgcgatatc gcgcgcgccc ccgcgacgtt ctccgccagc gacgcccggc
     5881 gcggaatggc gatcagctgg ttgatatgcg ggttgtgctc cagaaggacc cggtagtccg
     5941 cgagggtcag atagtcgatg acggcctccg gatgacgttc cgtgatggcc gccacgagcg
     6001 gcgtggtgag gacgatatcg ccgatggagc tgaaccggat gatcagaacg cgcatgccga
     6061 tggttacggt tcggactggt agtagcggtt ttccgacccg ttgcccagat cgggccgcag
     6121 gctcctcgcc tgatgcaaat agacgtggac catctcctcc tgccgcttgt cggaattcac
     6181 atgcaatagc acgcggatgc acgaggacag gctccccggg acaccgatct cgcgcgtgca
     6241 aaacagcggc gtatagatcc atcccagacg gcgcgcggcg accgcgggaa actcggcgct
     6301 tacgtcgtcc gtgaccgaaa agatcgccga cgccacgtcc tcgagctcga cgccgttaag
     6361 cgcgaccagc cgctccagca gttccgtcgt gcgcgtgaga atgtcctcgc gcgtattggc
     6421 atcgaccgtg gttgcgcccc gtatccctcg taccgccatg tctacccctt gtcgtcgtat
     6481 tgaacaatcg ctcttcgtca tcgcgcgcga tgcgcgccgc gcgtcgccgg atcaaactta
     6541 cgcctaaacc aatgccattc tcattcgccc aacgcaatat ccgcgccgat gccgcacatg
     6601 agctcccgga agcccgggaa ggtcaccccg accgcctcgg cggtgtcgat gaccgtcgtc
     6661 ccctccgcag cgcccgccgc cacggcgagc gccataacca cgcgatgatc gccgtggccg
     6721 tggacctgcg cgccgcgcaa tcggctccgc cggacgatga gcccatccgg aagctcctcg
     6781 atatcggcgc ccatgctccg cagttcgcgg cacatcaccg cgatgcggtc ggtctccttg
     6841 aggcgagcct gcgggacgtt caccaatcgc gtctccccgc tcgcgaaaca accggccacc
     6901 gcgagcgcgg gcagcgaatc gggcatggcg ttcagatcga acacgccccc cgcgagcgcc
     6961 cccggagcaa tggagatgga tcgctccgca atctcgatca cggcccccat cgcgcgcaac
     7021 accgacacga cctccttgtc cccctgcgta tcggagaaat cgaggccggt gagcgtcacg
     7081 cccgttcccg cgatggccgc ggccacgagg aaaaacgtcg ccgaggagaa atccgccggc
     7141 acatattcac gaaacgcggg ataccgctgc ccccccggca ccgtgatgcg cgcaagattc
     7201 tcgttccggt accgtatccc cagacgatcg agccagccga gggtcatgcg gacatacggg
     7261 acctcgtaca ggagggggat gttgataata gtttcggccg aaaccagcgg cgcggcgaca
     7321 aggagcgccg tcaggtattg agaggtcacc gcctcgatgg acgtttcccc gccgcggagc
     7381 ggcccgccga tcacgacggg cggtttgccg tttgaccgcg tcgataccgc actcccgccc
     7441 agatcgccga tggcccgcag gagggcgtcg gccggccggt tacggatctg ggcgtcgccc
     7501 gtgaacaccg tgtgccccgt gcagagggcc gccaccgcaa gcccgatata gagcgtggtc
     7561 ccggagttac ccacgtcgat gacgttgtcc ggaatcgccg gagcgccgcc catccccgtc
     7621 acggtccacg cgcccccggc gtccaacacc tccgcgccga aggcgcggat catgtccacg
     7681 catgaacgcg tgtcggacga ggacagcggc gcctcgatga ccgaggtccc ctcggcgagg
     7741 agcgcgaaga cgagcgcgcg gatcgtatgc gacttcgacc cggggatggc gaccgttccg
     7801 gacagtgttg atggattgac gatatagttc acggtgttcg tgcgcgcggg tgttacccgc
     7861 ataacgttgc gcgcagaatc accgtcggga gggatttgtc aatagttatt tacgaaggag
     7921 tttgccgccg ccgttgaaac gccatgtccc gttgtcgcgg acccaggtgt cgtcccagtg
     7981 ttccaccacg ccctcgctga actccagccg catcctgacg atcgcggtgc cgttcttgat
     8041 gtccattcca tcaatccggt actgcctgat ctgattgccc tcctcgctga agagggcatt
     8101 gaaattccgt acatagttgt cctcgggatg gccctgccga taggcgggcg cgcacagcag
     8161 atatgatttc tcgtactcgc ccgttttaat cagctcccaa tacgcctcga cgctcttttt
     8221 cagttcgtcc ttgcttccgc aggctccgag aacgcaacta gcgataatta gaaaagcgag
     8281 tattcttcca tgcatcatga cgcccctccg cgcaaaaaag acacttctgg ggtctgtatc
     8341 ggcattcgaa agagcgtcgc cccaatcttt tttactcctc gcccgcggtg atcgctccgc
     8401 gctccccgac gggaagctcc tg
//
LOCUS       DAORHF010000164         8422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_7979_length_8422_cov_4.311820, whole genome shotgun sequence.
ACCESSION   DAORHF010000164 DAORHF010000000
VERSION     DAORHF010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8422
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7979_length_8422_cov_4.311820"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="PKJ16_12865"
     CDS             <1..989
                     /locus_tag="PKJ16_12865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020880098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HNV47927.1"
                     /translation="AIQGGKTHYTHAMGLLELRIAICEHYRAEYGVTIAPEQVMVTSG
                     TSPAMLLMMIALLENGDEVVISNPHYACYPNFIRSVGGTCIEVRTFPEDGFQYRPEDI
                     KRAITKRTSGIVINSPSNPTGIVMTEEQMRQVAAFEDQYIISDEIYHGLVYKGAQHSI
                     LEFTDRAFVINGFSKLYAMTGWRLGYLIFPLEFSDTMQRIHQNFMISANSFVQWAGIA
                     ALTETAADIERMKAIYNERREYMIARLREIGFTIHVEPTGAFYVFADARAFCEDSYRE
                     AFEIVDAVKVGVTPGVDFGSGGEGFLRFSYANSMENIREGLDRVERYFRGAR"
     gene            986..2572
                     /gene="lnt"
                     /locus_tag="PKJ16_12870"
     CDS             986..2572
                     /gene="lnt"
                     /locus_tag="PKJ16_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HNV47928.1"
                     /translation="MRRTTAALALLSGFLYVCSFPGVSVPGLIFLALVPLIIALHRAI
                     SLREAFLAGMIFGMAHIIGMTYWLYYAIHVHYGGSLLVTVLIIVFAAALPVGLHAGVF
                     SLGFAYFRSGRIVFSALAVPALWVIVDYLRTISPVTLPWGLPAYAAIDCTRFVQMADI
                     AGVSGVTFVIVMVNACVAALIIRSMDMSGALHVREFARFCFAQSGWRHIVRMNWPIAL
                     IVVLAIIVPILYGTAMIARWSHSGTVGPDVGPAIAARIVQGNHGHKERWSDDTFHGRL
                     DVYTAMSNESYRTGDRRIVVWPETILNSPARVTPALLEKLIGFMGPQTVLVAGGVRRA
                     PKDALYNSVYVLSGSGHARWFDKNILLPFSETRPLSIGVLGRYYEAPDEFAPGTTPPI
                     VRSDHAALGVSICFELIYPWFVRASVQKGADLLVNVSNDAWFGDSAEPRQHLDIARFR
                     CIENRRYMLRASNSGISAVIGPTGAVLARSGLFTRESVDGAVRAIPARSIYTRAGETA
                     VIGSLVVVLAMLVLSLFKKD"
     gene            complement(2569..3570)
                     /locus_tag="PKJ16_12875"
     CDS             complement(2569..3570)
                     /locus_tag="PKJ16_12875"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HNV47929.1"
                     /translation="MDKKKKIIIAAAGILIVVIILIQVLKKDDPMGAVNIIDGSQKID
                     AEDYKLFFKNIRFEDGPTDKYFSKSIANQFTIKFFKFLQRKFGKMSFEDHLKAVEDYL
                     NSTMDPRRAAEMYALYKKFAQYERSLMDTSKKWPTPKSPEDMIKYLQDVQEYRRSFFG
                     KELADELFGTMVKTQEYGIRKNAIVNDKNLYGEEKEKRLKELKSKMWGADASAIDSEL
                     RPYDQYQEKLSVYDRDLSELPEAERTNRIKEYRNQFFTPEVVARLEKVDEQLANEKQA
                     EEEYRRREQRIIADPNLTPEQKDSQIMYLQNEMFGEEADAFRRREAIRQGLEEMKTP"
     gene            3710..4711
                     /locus_tag="PKJ16_12880"
     CDS             3710..4711
                     /locus_tag="PKJ16_12880"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HNV47930.1"
                     /translation="MKQALIGIAVTVLTVIGIPSFGVAEVKKVSGEAGIERIFGYTQY
                     RIGGKVTPAKGKAGTLHYPVSELQFPLDVYWLSIGGMVKFTDDVRANGSFKTNVNRDA
                     GKMKDSDWGYWYLQGKTWADYRTLDVYSLSDASLKGYILDADIQFRAFEMEPFTLHLG
                     VGFMYQYFGYDISNLDQWYPSYNTYRAYLSPTYSNHDYVRDLIMTYTVEYFIPYLELV
                     PELKVGAAALLSAQLGLAPYVIARDVDDHILRSKVMDGSATGWAFMASIKGEYYIDKT
                     VSVSLAAGYLWIWTKGKQSQSFYETTDESTAGYIGTIENRIITEQYYVRIGAAYAFK"
     gene            4747..5754
                     /locus_tag="PKJ16_12885"
     CDS             4747..5754
                     /locus_tag="PKJ16_12885"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HNV47931.1"
                     /translation="MIALWAMSVAPFQGANHTALAQDNRAIVEIGVEKMSGYTQYRIG
                     GRGTLSDGHSREYPSPLSELTFPLYVYFFSIGFIAEFEEDFRVSARAKTNIDTRAGKM
                     RDADWGVYYIDGWPEDSNGVPQPGNTPARIDTLDILSRSDAELRAYIGECDFLYFAYN
                     GRTLQLGGGASFTYYSFSYVVSNFDQWYPSSGYYYGTSFPSDIERRVALTYDIQYYVP
                     ALLLAGGVKIGREASILAQASFSPYVRAFDFDVHIARDKLCRSSYTGVSFGGSLAARW
                     NVTREVSIGLFGQYQYIQARGEQRQYFYDNTGNYFIASIDAWVKSEQVSGGLTVGYSF
                     E"
     gene            complement(5770..6501)
                     /locus_tag="PKJ16_12890"
     CDS             complement(5770..6501)
                     /locus_tag="PKJ16_12890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HNV47932.1"
                     /translation="MPAFPVDIIPVVILSIAPGIFWLWYFYKRNTLEPEPKALVAQVF
                     FFGMICLAPAALIEQPFRPYPFVQTVIVAPIVEELIKFLLVYLWIARRTDFNEPMDGI
                     VYASAAALGFASAENALYIASAYLFPQIALDIHDPAYGLQWVWKIYLVRAFLTVPGHA
                     VWSAMWGYAIGWARFVDPARTRPLIVKGVLISIVLHAFFNFLVNDYPIGAAGMLFLVP
                     LMWKMLDDRIRRAIEQSPFRKNVDE"
     gene            complement(6514..6891)
                     /locus_tag="PKJ16_12895"
     CDS             complement(6514..6891)
                     /locus_tag="PKJ16_12895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV47933.1"
                     /translation="MRKVFIINEHAKWIELLTFLVSSLGHAVEHLADPLDLFDRVITD
                     RPDLVIMDIHYEYIDGIFLVEKYMVIPDCRKVPILVVSSKNDPLSLVDSIERGAADFL
                     SVPINEDQLIEKVKKLLPPTAPA"
     gene            complement(6898..8166)
                     /locus_tag="PKJ16_12900"
     CDS             complement(6898..8166)
                     /locus_tag="PKJ16_12900"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HNV47934.1"
                     /translation="MDDVKIDLEKYRELPPTFLQNLSGDKMLTFDILDQNGEVVFRSG
                     EAIQVDAIQKSIAAGSKFFIPRAVEPTTGRYDYEIIPVETMKGIAAETAKTYGAIRSS
                     GVMSYEQYVASHEQFAAIVQGLRQEDKNGGLLSLMREIENFDYYTYVHSVNVGVLAMI
                     LMYKLGYNKDEVRTLSTAGYLHDIGKLRVPKDIIEKPGRLSKSEFEAMMDHTRDGFRI
                     LDAIVAPGGQKAVPSIIKLTALFHHRKFRNRGYPYRSFRDKKSEQFYLEIPEEVRLIS
                     ICDMFDAITTSTPYRKGVSHEEGLQFILNLSNYHFTTNDVYRFLKPMIHTLNKGKNIL
                     AEGDFIMVESTARSTDDSAKKQLVYEFARVMKLYKGRSLEPHIRIFYDISKKKKIHPI
                     EVDLRHDHYRRIVRILKSDRMLDMMRTVYS"
BASE COUNT         1597 a         2370 c         2575 g         1880 t
ORIGIN      
        1 gggccatcca gggcgggaaa acgcactata cccacgcgat ggggctgctt gagttgcgga
       61 tcgcaatctg cgaacattac cgcgccgaat atggagtcac gatagcaccc gaacaggtga
      121 tggtcacctc cggcacgtcg ccggcgatgc tgctcatgat gatcgccctg ctcgagaacg
      181 gcgacgaggt ggtcatctcg aacccgcact acgcctgcta cccgaacttc atccgttcgg
      241 tgggcggcac ctgcatcgag gtgcgcacct ttcccgagga cggcttccag taccgcccgg
      301 aggacatcaa gcgcgcgatc acgaagcgca cgagcgggat cgtcatcaac tcgccctcga
      361 acccgaccgg catcgtgatg accgaggagc aaatgcggca ggtggcggcc ttcgaggacc
      421 agtacatcat ctcggacgag atctaccacg ggctggtgta caagggggcg caacactcga
      481 tactcgagtt caccgatcgc gccttcgtga tcaacgggtt ttcgaagctg tacgccatga
      541 ccgggtggcg cttgggctac ctcatcttcc cgctcgagtt ctccgatacc atgcagcgga
      601 tccaccaaaa cttcatgatt tcggccaata gcttcgtcca gtgggcgggg atcgccgcgc
      661 tcacggagac ggcggccgat atcgaacgga tgaaggcgat ctacaacgag cggcgcgagt
      721 acatgatcgc gcgcctgcgt gagatcggct ttaccatcca tgttgaaccg acgggcgcgt
      781 tttacgtttt cgccgacgcc cgcgccttct gcgaggattc gtaccgggag gccttcgaga
      841 tcgtcgatgc cgtcaaggtc ggcgtgacgc cgggggtcga tttcggatcc ggcggggagg
      901 gcttcctacg attttcgtac gcgaattcga tggaaaacat tcgggaggga ctcgaccgtg
      961 tcgagcgcta tttccggggc gcaagatgag gcgcacgact gctgcactcg cgctgctctc
     1021 cgggttcctg tatgtgtgtt cttttcccgg cgtatcggtc ccggggctca tcttcctggc
     1081 gctggtcccg ctgatcatcg cgctgcatcg cgcgatctcc ttgcgcgagg cattcctcgc
     1141 cggaatgatc tttggcatgg cgcatatcat cggcatgacc tactggctgt actacgcgat
     1201 ccatgtccat tacggcggat cgcttctggt cacggtgctc atcatcgtat tcgccgctgc
     1261 gctcccggtt ggattgcatg cgggtgtgtt ttcgcttggg ttcgcgtatt ttcgctccgg
     1321 tcgcatcgtc ttttcagcgc tcgcagtgcc ggcgctctgg gtgatcgtcg attatctgcg
     1381 gaccatttct cccgtcacgc tgccgtgggg acttcccgcg tatgccgcaa tcgattgcac
     1441 gcgcttcgtt caaatggccg acatcgccgg cgtttcggga gtcaccttcg tgatcgtaat
     1501 ggtgaacgcg tgcgtcgcgg cgctcatcat ccgctcgatg gacatgtcgg gcgcattgca
     1561 tgtacgtgaa ttcgcacgct tctgtttcgc gcaaagcggc tggcggcata tagtgcgaat
     1621 gaactggccg atcgcactca tagtcgtgct cgcaataatc gttccgattc tgtatggaac
     1681 cgccatgatt gcgcgatggt ctcattccgg gacggtcggg cctgatgtcg gacccgcgat
     1741 tgccgcgcgc atcgtgcagg gaaatcacgg gcacaaggag cgctggagcg acgatacctt
     1801 tcacggccgc cttgacgtct acaccgcgat gtccaacgag tcgtatcgca ccggcgatcg
     1861 tcgcatcgtc gtatggcctg aaacgatctt gaactcgcct gccagagtga ctcccgctct
     1921 cctcgaaaag ctgatcggct tcatggggcc gcagacggtg ctcgtcgcgg gcggcgtccg
     1981 gcgcgcaccg aaggatgcat tgtacaattc ggtgtatgta ttatcgggaa gcggccatgc
     2041 gcgctggttc gataaaaaca tcctgctccc attctccgag acccggccgc tgtccatcgg
     2101 cgtgctgggc cggtactacg aagcgcccga cgaattcgcc cccggtacca cgccgcccat
     2161 cgttcgcagc gaccatgccg cgctcggcgt gtccatctgc ttcgagctca tctatccgtg
     2221 gtttgtgcgc gcatcggtcc agaagggcgc cgatctgctg gtaaacgtat caaacgatgc
     2281 ctggtttgga gattcggccg aaccgcgtca gcatctcgac atcgcccggt tccgttgcat
     2341 cgaaaaccgc cgctacatgc tgcgggcgtc caacagcggg atttccgcgg tcatcggtcc
     2401 cacgggcgcg gtgctcgcga ggtcggggct gttcacgagg gaaagcgtcg atggggcggt
     2461 gcgcgccatt ccggcgcgga gcatctacac ccgcgccgga gagacggcgg taatcggttc
     2521 gctcgtggtg gtcctggcga tgctggtcct gtcgctcttt aaaaaggact agggcgtttt
     2581 catttcctcg aggccctggc gtatcgcctc gcgacgccgg aacgcgtccg cctcctcgcc
     2641 gaacatctcg ttctgcaggt acatgatctg cgagtccttc tgctcggggg tgaggttggg
     2701 gtcggcgatg atccgctgct cgcggcggcg gtattcctcc tcggcctgct tttcgttcgc
     2761 aagctgctcg tcgaccttct cgagacgcgc gacgacctcc ggcgtgaaga actggttccg
     2821 gtattccttg atgcgattgg tccgctcggc ctcgggcagc tcggaaagat cgcgatcgta
     2881 tacggaaagc ttttcctggt actggtcgta cggccgcaac tcgctgtcga tggccgaggc
     2941 gtccgcgccc cacattttcg atttaagctc cttgaggcgc ttctccttct cctcgccgta
     3001 gaggtttttg tcgttgacga tcgcgttctt gcggattccg tattcctggg ttttcaccat
     3061 cgtcccgaaa agctcgtcgg cgagctcctt tccgaagaag ctccgccggt actcctggac
     3121 atcctggaga tacttgatca tgtcctcggg ggatttcggg gtgggccatt tcttcgaggt
     3181 gtccatgagg ctccgctcgt actgtgcgaa ctttttgtac agagcgtaca tctcggcggc
     3241 gcgacggggg tccatcgtgg agttgagata gtcctccacg gccttgagat ggtcctcgaa
     3301 gctcatcttg ccgaacttgc gctggagaaa cttgaaaaac ttgatggtga actggttcgc
     3361 gatggatttc gagaaatact tgtcggtggg cccgtcctcg aaccggatgt tcttgaaaaa
     3421 caacttgtag tcctcggcgt cgattttctg agagccgtcg atgatattta cggcgcccat
     3481 cgggtcgtct tttttgagga cttggatgag gatgatgacg actatcagga tgccggcggc
     3541 ggcgatgatg atctttttct tcttgtccat gaagtaccaa tcctttgtgg gtttgtgcct
     3601 gcgggagaat tgtacgggca atttcttatg tcaaataatt ttttcgttga gcccggaggg
     3661 agaccggtcc ataatggatt atccgacgtc acggcaggaa tgtaaacgaa tgaaacaggc
     3721 actaattggc atcgccgtaa ccgtcctcac ggtgatcggc ataccctcgt tcggcgtcgc
     3781 cgaggtcaag aaggtaagcg gcgaagcggg gatcgagcgc atcttcgggt atacgcaata
     3841 tcgaatcggc ggaaaggtga ccccggccaa aggcaaggcc ggcaccctgc attacccggt
     3901 gagcgaactg caattcccgc tcgacgtcta ctggctgtcc atcggcggga tggtgaaatt
     3961 caccgacgat gtccgcgcta atggaagctt caagacgaac gtaaaccgcg atgcggggaa
     4021 gatgaaggat tcggactggg gatactggta cctgcaggga aagacctggg ccgactatcg
     4081 tacgctcgac gtctattccc tgagcgacgc aagcctcaag ggctacatcc tcgatgccga
     4141 tatccagttc cgcgcgttcg aaatggagcc gttcaccctg catctgggcg tcgggttcat
     4201 gtatcagtat ttcgggtacg atatcagcaa tctggaccaa tggtatccat cgtacaatac
     4261 ctaccgtgcg tatctgtctc cgacgtactc gaatcacgat tatgtgcgcg atctcatcat
     4321 gacctatacc gtggagtatt ttattcccta tctcgagctg gtcccggaac tcaaggtcgg
     4381 ggccgccgcg ctcctgtcgg cccagctggg tcttgcgcca tatgtgattg cgcgcgatgt
     4441 ggacgaccac atcctccgat cgaaggtcat ggacggcagc gcgacgggat gggcgttcat
     4501 ggcctcgatc aagggcgagt attatatcga caaaacggta tcggtctccc tagcggccgg
     4561 ttatctctgg atctggacga aaggaaaaca gagccagtcg ttctatgaaa cgacggatga
     4621 aagcaccgcg ggatatatcg ggaccatcga gaaccgcatc atcaccgaac agtattacgt
     4681 caggattggg gccgcgtacg catttaagtg accatgcgct ttcctcgctc gacagcagtc
     4741 atcggcatga tcgccctgtg ggcgatgagt gtcgcgccgt ttcagggcgc gaatcatacg
     4801 gcgctggcgc aggacaaccg cgcgatcgtc gagatcggcg tcgaaaagat gtcggggtat
     4861 acccagtacc ggatcggcgg acgcggcaca ttgtcggacg gccatagccg tgagtatccc
     4921 agccccttaa gcgagctcac ctttcccctg tacgtgtatt ttttctccat cggattcatc
     4981 gcggaattcg aggaggattt ccgagtgtcg gcgcgagcga aaacgaacat cgacaccagg
     5041 gccggaaaaa tgcgcgatgc tgactggggg gtgtattaca ttgacggctg gccagaggac
     5101 tcgaacggcg ttccacagcc ggggaacacg cccgcgagaa tcgacacgct cgatatcctt
     5161 tcccgcagcg atgccgaact tcgggcgtat atcggcgagt gcgactttct gtacttcgcg
     5221 tacaatggac gcacgctgca gttggggggc ggggcgtcgt ttacgtacta cagcttttcg
     5281 tacgtcgtca gcaatttcga tcagtggtat ccgtcatccg gctactatta tgggacatcg
     5341 ttcccgagcg atatcgagcg acgcgtggcc ctcacctacg acatccagta ctatgtcccg
     5401 gcgctcctgc tggccggcgg cgtgaagatc ggacgcgagg cgtcgatcct cgcacaggca
     5461 tcgttctccc cgtatgtccg cgcgttcgat tttgacgtgc atatcgcgcg ggacaagctg
     5521 tgccgctcat catatacggg cgtgtcgttc ggcgggtccc tcgccgcccg gtggaatgtc
     5581 acgcgtgagg tctcgatagg ccttttcggt cagtaccagt acattcaggc gcggggtgag
     5641 cagcggcagt acttttatga taacaccggc aattacttta tcgcgtccat cgatgcgtgg
     5701 gtaaaaagcg agcaggtttc cggcgggttg acggtgggat attccttcga gtgatgcatt
     5761 gccgttgcct cactcatcga cattcttccg aaacggcgat tgttcgatgg ctctcctgat
     5821 ccggtcgtcc agcattttcc acatcagcgg caccaagaac agcatccccg ccgcgccgat
     5881 gggataatcg ttcaccagaa aattgaaaaa cgcgtgcagg acgatggaga tgagcacgcc
     5941 cttcacgatg agcgggcggg tccgcgccgg atcgacgaaa cgcgcccagc cgatggcgta
     6001 tccccacatc gccgaccaca cggcgtggcc cggcaccgtg agaaaggcgc gcaccaggta
     6061 gatcttccac acccattgca ggccgtaggc cggatcgtga atgtcgaggg cgatctgggg
     6121 gaacaggtag gcgctggcga tatacagcgc gttttccgcc gacgcgaagc cgagggccgc
     6181 cgcgctggcg tacacgattc cgtccatcgg ttcgttgaaa tcggtccggc gggcgatcca
     6241 caggtacaca agcaggaact tgatcagctc ctcgacgatc ggcgcaacga tgaccgtctg
     6301 aacgaaggga tatggtcgaa acggctgttc gatgagcgcg gcgggcgcca ggcagatcat
     6361 gccgaaaaaa aacacctgcg cgacgagcgc cttcggttcc ggttcgagcg tgttgcgctt
     6421 gtagaaatac cacagccaga agatccccgg cgcgattgaa aggattacga ccgggatgat
     6481 atcgacgggg aaggccggca tcgtgccgct cgatcaggcc ggggctgttg gcggcagcag
     6541 ttttttcacc ttctcgatga gctggtcctc gttgatgggc accgagagga agtccgcggc
     6601 gccgcgctcg atcgagtcca ccagcgagag cggatcgttt ttcgacgaga ccaccaggat
     6661 cgggaccttg cggcagtccg gtatcaccat gtatttctcc acgaggaaga tgccgtcgat
     6721 gtactcgtag tggatgtcca tgatcaccag atcgggccgg tcggtgatga cgcggtcgaa
     6781 cagatcgagc ggatcggcca agtgctccac ggcgtggccg agcgacgaaa cgaggaaggt
     6841 gagcagctcg atccatttcg cgtgttcgtt gatgatgaat acctttcgca tggcgtccta
     6901 cgagtacacg gtccgcatca tgtcgagcat gcggtcgctt ttgagtatgc gcacgatgcg
     6961 ccgatagtgg tcgtggcgca ggtcgacctc gatggggtgg atcttctttt ttttcgagat
     7021 gtcgtaaaag atgcgaatgt gcggctcgag cgaacgacct ttgtagagct tcatgacgcg
     7081 cgcgaattcg tatacgagct gtttcttggc cgaatcgtcg gtcgagcgcg cggtggattc
     7141 gaccatgata aaatcgccct cggcgaggat gtttttcccc ttgttgagcg tgtggatcat
     7201 gggcttgagg aagcgataga cgtcattggt ggtaaaatgg taattcgaga ggttgaggat
     7261 gaactgcagc ccctcctcgt gcgagacgcc cttccggtag ggggtggagg tggtgatggc
     7321 gtcgaacatg tcgcagatgc tgatgagccg aacctcctcg gggatctcga gatagaattg
     7381 ctcactcttc ttgtcgcgga agctgcgata gggatagccg cggtttctga acttgcgatg
     7441 atggaagagc gccgtgagtt ttatgatgga ggggaccgcc ttctggccgc ccggggcgac
     7501 gatcgcgtcc aggattcgga atccgtcgcg ggtgtggtcc atcatcgcct cgaattcgct
     7561 tttgctgagg cggcccggct tttcgatgat gtccttcggg acgcgcagct tgccgatgtc
     7621 gtgcagatag ccggcggtcg agagggtgcg cacctcgtcc ttgttgtagc cgagcttgta
     7681 catcaggatc atcgcgagca cgcccacgtt gacggagtgc acataggtgt agtagtcgaa
     7741 attctcgatt tcacgcatga gggagagcag cccgccgttc ttgtcctcct ggcgcagtcc
     7801 ctgcacgatg gccgcgaatt gctcatgcga ggcgacgtac tgctcatagg acatgacgcc
     7861 ggagctgcgg atggccccat aggtcttcgc ggtttcggcc gcgatgccct tcatggtttc
     7921 caccgggatg atctcgtagt cgtaccgacc cgtggttggc tcgacggcgc gcggaatgaa
     7981 aaatttcgat ccggccgcga tgctcttttg aatggcgtcg acctgaatcg cttcaccgga
     8041 ccggaacacg acctcgccgt tctggtcgag aatgtcgaag gtgagcattt tatcgccgct
     8101 caggttttgg agaaaggtcg gcggaagctc gcggtatttt tcgaggtcga ttttaacgtc
     8161 gtccatttgc gatgcgcgtg gcgtgtccgt cagtgttcgt gaagctatcg agtgtcttgt
     8221 gagatcatgg cgcaggattc accgtctgtc aagaataatc tatgggcgaa ttggccgcct
     8281 gcggatgaca cgcggagcga atcagcgtat atggcgagaa tcgctcaccc actcgtccgg
     8341 atgcgatggg ccttcccgca tcgattttcg cgaagagagg ttcttcgcgc cgatatatcg
     8401 gcgctcctgc ggatgggagc gc
//
LOCUS       DAORHF010000165         8269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8185_length_8269_cov_5.587290, whole genome shotgun sequence.
ACCESSION   DAORHF010000165 DAORHF010000000
VERSION     DAORHF010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8185_length_8269_cov_5.587290"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PKJ16_12905"
     CDS             complement(<1..373)
                     /locus_tag="PKJ16_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47935.1"
                     /translation="MIQTLRSLTWLDYYLFALLDPRRLAEAVVRAEPRPLPLGAAVMA
                     AVAIGEIIALSFLSAQTEFFFVKLTYGWIFTFLVMALKVAVLSALFDFSCQIMGCEGR
                     GVPILNVVMFSLSPRLFLLPLV"
     gene            complement(370..1323)
                     /gene="sppA"
                     /locus_tag="PKJ16_12910"
     CDS             complement(370..1323)
                     /gene="sppA"
                     /locus_tag="PKJ16_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HNV47936.1"
                     /translation="MDRNRKILLSILALLVLAVVLAIIDISLTMQSPSRRVRSISSSS
                     IAPGVGVVRISGPIEFAGPADNPFMPRQGAESVLKRLDELMADSRIKAIVVRINSPGG
                     TVAATQEIFQKLMKARKGNMVLVASMGDVAASGGYYAASACGFIMANPGTLTGSIGVI
                     AVSPNLKGLFEKLGIRMTVIKSGRYKDLLSSFRDLGEDERLLLQQMIDSTYKRFLKDV
                     SLGRNVPISDIEPVADGRVMTGEAALGAKLIDGIGGFEEALEKARKMAKLPDDAPVYD
                     DVRSPIEQLFMSVDGAIGRRPALHDMVPLPKHHLLEYRFLP"
     gene            complement(1455..1934)
                     /locus_tag="PKJ16_12915"
     CDS             complement(1455..1934)
                     /locus_tag="PKJ16_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47937.1"
                     /translation="MSRHVLMLTLAASVIATGVLAPPAAAPSRVGRAPEGPIVRTAHG
                     IVTAAVRSVRTFRREMGPRACWEDVVMELVVRRVVKGALREGMALSFSYRVLLDDAAP
                     CPRLYYVNPPHAALGESVRSGAREIIAAVERLPGSEQYRVTGTFDIERMEWVRATMR"
     gene            complement(1944..3584)
                     /locus_tag="PKJ16_12920"
     CDS             complement(1944..3584)
                     /locus_tag="PKJ16_12920"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HNV47938.1"
                     /translation="MMKRITTLCTMALAALLGSFFFSPDARARSVQIELPIEKTAGAE
                     PRIRTSQAPNTVRKSYVPSARASQGDGFVTERHIVVTALDARGKVLYRRAMLDPRQLH
                     VDDFLEKGGAKKRPAGPGKIIEIDSTILTNIPDNPAIETVHVHKPVWNGKKFDLVLLT
                     SGAMPKKGAAKSADQGDINIKSVRGAGEARVTTIRSTGAPAERVDIVLLGDGYTASQM
                     KLWEADAERIANELFKHEPFKSHEKFFNVHRVDLASAEPGAGWSTPFSRNNAFGSYYN
                     CNNIARLLWLDTAHADRIINSVLQADTQDIKIVLVNDGRYGGGGGNYATVSARASGMI
                     DIILHELGHSFAGLADEYSSQGYPCGDVSVEPAEPNVTTIASRDSVKWRKFADAGVFE
                     GAKYCERGLFRPYENSKMRSLSGAFHELHIAAFRDRIMGIVKSGAAEGGDDGASKDGA
                     GDAGARDEGAAAQPNREFKYRGGVFVNTAGALWEARKDGGAVNSFREISRDGNYIYIR
                     DEKRDLYLGLPIFETTDWFYKFTRRGGRYIYEKLTALP"
     gene            complement(3629..4891)
                     /locus_tag="PKJ16_12925"
     CDS             complement(3629..4891)
                     /locus_tag="PKJ16_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47939.1"
                     /translation="MDGRAAGGGRRKTESGRRLSRMIADGYLDLHRRANEGAFVVWIA
                     IVVPAEIFAGFENVAYAVPESHAALSAGKGVGTVQCQKAEERGYSADLCSYARIDIGT
                     AFDGGKDSPTFGLPRPHLLVSNNNNCSLLVKWFDVYRREWGVPHFIVDVPFCYGPQSD
                     RDTDYIAAQYRDLIREIERLSGQRFDIERVREAVRHSCDAMREWKRFLRAAAHRPSGI
                     TVFDSFVQMAPILTARGTPALAEHFRLLADETEGAIADGRYPVPDERYRLLWDNIAPW
                     HQLSGMSSRLAALGVNLIAASYTYCIGSLEGSVQLFPYDGADPLRYLARIQNFSVCPQ
                     GLALRFEAMRAAIGTMGVDGVVFASNRSCKVYSIMQMDLLDRVTRECGVPAVMIDVDH
                     ADARAYSEEGAFTRIEALLESIDMKRAG"
     gene            complement(4891..5997)
                     /locus_tag="PKJ16_12930"
     CDS             complement(4891..5997)
                     /locus_tag="PKJ16_12930"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV47940.1"
                     /translation="MIETIRACREKGTPVVGCFPLYPPLELLHAMGLRPVVLWELKGI
                     VKSTPLSDRHLQPFACSVARHLVEIVLSDYGAILDGLLMYNACDTLRNLPEIIERGLG
                     AQGRELPIIKLHIPAIPAERTEASEYLARRMDALVAECEAAFGVHFSSERFLESQRRY
                     GEMRALLRRLEALVGEGRLLYRAFSRINRFGYLVGVEEQVKRLRTAVDDCAAVSINAS
                     ATIPIVVSGILPPPPAMIDLIERCGLRVVANDVATQRRSYGYQPPEDGDPGGYYADFY
                     RRHHPCTTLLPTADRRVDTILSLVEESGARGLVFIGEKFCEYESLEFPHIEGLLRERG
                     VSSLRLEMAIDDYENCAAFATRIEAFAELLKGGR"
     gene            6126..7280
                     /locus_tag="PKJ16_12935"
     CDS             6126..7280
                     /locus_tag="PKJ16_12935"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HNV47941.1"
                     /translation="MLTRREFLSTLTRASAAGVLAGLGAGACAHAARSPHYAPVGRRI
                     THRRFTLRMKGTWSIARGSMDSKDIVIVELERDGYIGRGEAAPSSFYGESAESSTAFV
                     QAVTPLLESADWRDIDAFEKAIVRRGAPDRGGLCAVTTALLDWNARRRGLPLHRHLGI
                     DARTMPRSTFSIGISARGEMLRKVRAASAYPLIKIKVGFDGDIAIIEAIRSISRQTIR
                     VDANGGWRSKEEALEKITALARLGVEFVEQPLPVDRIDDARWLTARSPLPIIADEGVR
                     TVRDIAPLTGACHGINIKLMKCGGIPEALRMIALARSHGMRVMMGCMIESSLGITAAA
                     HIAPLVDFADLDGNLLVANDPFARGVRITNGLLVLPGGPGIGVEQAPFTA"
     gene            7709..>8269
                     /locus_tag="PKJ16_12940"
     CDS             7709..>8269
                     /locus_tag="PKJ16_12940"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV47942.1"
                     /translation="MHFDTDTRTERTSPGVYSAAISDRWSINNNPNGGYLMAVMSRAL
                     LTQSDFSSTPIVTANYLARCVPGPATIEVREISRTRQFHRFEASIIQDGAERVRALAT
                     FASGGGDCSINRYEASPPELAPREDCVLIPTIPTYTLFGELDVMLDPSCAGWMQNKHV
                     ERSENKGWMRFKNERPYDLPAVLLIAD"
BASE COUNT         1478 a         2778 c         2614 g         1399 t
ORIGIN      
        1 tcaccagcgg caggaggaag aggcgcggcg agagcgaaaa catcaccacg ttcaggatgg
       61 ggacgccccg cccctcgcag cccatgatct ggcaggagaa gtcgaagagc gcggacaata
      121 ctgccacctt gagcgccatg accagaaacg tgaatatcca gccataggtg agcttgacga
      181 agaagaactc ggtttgcgcg ctcaggaacg acagcgcgat gatctcgccg atggcgaccg
      241 cggccatgac cgctgcgccg agcgggagcg gacgcggctc ggcccgcacg acggcctcgg
      301 cgaggcggcg cggatcaagc agcgcgaaca ggtaatagtc gagccaggtg agcgagcgca
      361 gcgtctgtat catggcagga accggtattc gagcagatgg tgcttcggca acggcaccat
      421 gtcgtgcagg gccggacggc ggccgatggc gccatcgacc gacatgaaga gctgctcgat
      481 ggggctacgc acatcgtcgt acaccggggc gtcatcggga agcttggcca tcttgcgggc
      541 cttctccagc gcctcctcga agccgccgat cccgtcgatg agcttcgcgc cgagcgccgc
      601 ctcgcccgtc atcacccgtc cgtccgcgac gggctcgatg tccgagatgg gcacattgcg
      661 cccgagcgac acgtccttga ggaagcgctt gtaggtcgaa tcgatcatct gctgaaggag
      721 cagccgctcg tcctcgccga gatcgcggaa cgacgagagc aggtccttgt accggccgct
      781 cttgatcacc gtcatgcgga tgcccagctt ttcgaagagc cccttgaggt tcggcgaaac
      841 cgcgatcacc ccgatggagc cggtgagcgt tccgggattg gccatgataa acccacaggc
      901 cgacgccgcg taatagccgc ccgacgcggc gacgtccccc atcgaggcga cgagcaccat
      961 gttgcccttg cgcgccttca tgagcttttg gaagatctcc tgcgtcgcgg cgacggtccc
     1021 acccggcgag ttgatgcgga cgacgatcgc cttgatgcgc gaatccgcca tgagctcgtc
     1081 gagccgcttc aacacgctct cggcgccctg ccgcggcatg aagggattgt ccgccggccc
     1141 ggcaaactcg atgggaccgg aaatgcggac gacgccgacg ccgggcgcga tcgacgagga
     1201 ggaaatcgag cgcacgcgtc gcgacggaga ctgcatggtg agcgatatgt cgatgatggc
     1261 caggacgacg gcgagcacca gcagcgcaag aatggacaag agaatctttc tgtttcgatc
     1321 catggaagct ccgattgtgc attgaatacg gctactgaac agatgccgcg attattagca
     1381 aggttttttt cccgagcggc cgcacggcga gatcacgcac tcaacgcatg ctgcgttcct
     1441 ggcgcggcat ccgcctagcg catcgttgcc cgcacccact ccatccgctc gatatcgaag
     1501 gtgcccgtca cgcgatactg ctcgctcccg gggagtcgct cgaccgccgc gatgatttca
     1561 cgcgcgccgg agcggaccga ctcgcccagc gcggcatggg gcggattcac gtagtacagc
     1621 cgcgggcagg gcgcggcatc gtcgagcagc acccgatagg agaacgacag cgccatgccc
     1681 tcgcgaagcg cgcccttgac gacccgccgc acgacgagct ccatgacgac gtcctcccag
     1741 cacgcacgcg ggcccatctc ccggcgaaac gtgcgcacgg aacgcacggc ggccgtgacg
     1801 atgccatgcg cagtgcggac gatcggcccc tcgggggcgc ggcccacgcg ggacggcgcc
     1861 gctgccgggg gcgcgagaac gcccgtcgcg atcactgacg cggcaagcgt tagcatcagc
     1921 acatgccgtg acatggcccg cgcctacggg agcgccgtga gcttttcgta gatatacctc
     1981 ccgcccctcc gggtgaactt gtagaaccag tcggtcgtct cgaagatcgg caggcccagg
     2041 tacagatctc gcttttcgtc cctgatgtaa atataattgc cgtcgcgcga gatctcgcgg
     2101 aaactgttga ctgcgccgcc gtccttgcgc gcctcccaga gcgcgcccgc ggtgttcacg
     2161 aacacgccgc cccgatactt gaactctcga ttcggctgcg ccgccgctcc ctcgtcgcgt
     2221 gcgcccgcat cgccggcgcc gtccttcgag gcgccgtcat cgccgccctc tgcggcgccg
     2281 cttttcacga tacccatgat ccgatcccga aacgcggcga tatgcagctc atgaaaggcg
     2341 ccggagaggg aacgcatctt cgagttttca tacggacgaa agaggccgcg ctcgcaatac
     2401 tttgcgccct cgaagacccc ggcgtccgcg aacttccgcc acttgacgct gtcgcgcgag
     2461 gcgatcgttg tcacgttcgg ctctgcgggc tccacggaaa catctccgca cggatacccc
     2521 tgggagctgt actcgtcggc aaggcccgcg aaggagtgcc ccagctcgtg aaggatgatg
     2581 tcgatcatcc cggacgcgcg ggccgagacg gtcgcatagt tcccgccgcc gccgccgtac
     2641 cgtccgtcgt tcacgagaac gatcttgatg tcctgcgtgt cggcctgcag gacggagttg
     2701 atgatgcgat cggcgtgggc cgtgtccagc cacagcaatc gcgcgatatt gttgcaattg
     2761 tagtacgacc cgaaggcgtt gttccggctg aagggcgtgc tccaaccggc gcccggctcg
     2821 gcgcttgcga ggtccacgcg gtggacgttg aagaacttct cgtggctttt aaacggctcg
     2881 tgcttgaaga gttcgttcgc gatgcgctcg gcgtcggcct cccagagctt catttgcgac
     2941 gcggtgtagc cgtctcccag gaggacgatg tccacgcgct cggcgggcgc gccggtggag
     3001 cggatcgtgg tcacgcgcgc ctctcccgcg ccgcggacgg acttaatgtt gatatcgccc
     3061 tggtccgcgg atttcgccgc tcccttcttc ggcatcgcgc cgctggtgag cagcacgaga
     3121 tcgaacttct ttccgttcca gaccggcttg tgcacatgca cggtctcgat ggcggggttg
     3181 tcgggaatgt tcgtgagtat ggtggagtcg atctcgatga tcttgcccgg gcccgcgggc
     3241 cgcttcttcg cgccgccctt ctccaggaag tcgtccacat gcaactggcg cggatcgagc
     3301 atggcgcggc gatagagaac cttccccctg gcgtccagcg ccgtgacgac gatatgccgc
     3361 tccgtgacga acccgtcgcc ctgcgacgcg cgcgcggagg ggacgtagct cttacgcacc
     3421 gtattgggcg cctgggaggt ccggatgcgc ggttccgcgc cggccgtttt ctcgatggga
     3481 agctctatct gcaccgaacg cgcgcgggcg tccggcgaga aaaagaatga gcccaggagc
     3541 gctgcaagcg ccatcgtaca aagcgttgta attcgtttca tcattgcgat cctccccgtc
     3601 gtgcggcctt gtattcgcgc gggaccgatt atcccgcgcg cttcatgtca attgattcga
     3661 gcagcgcctc gatgcgcgtg aaggcgccct cctcgctgta ggcgcgcgcg tcggcgtggt
     3721 ccacgtcgat catcacggcg ggcacgccgc actcccgcgt cacgcgatcg agaaggtcca
     3781 tctgcatgat cgaatacacc ttgcagctgc ggttgctcgc gaacaccacc ccgtccacgc
     3841 ccatcgtccc gatcgccgcg cgcatcgcct cgaagcggag cgcgagcccc tgcgggcaca
     3901 ccgagaagtt ctggatacgc gccagatagc gcagcggatc tgcgccatcg tacgggaaaa
     3961 gctggaccga accctccagg cttccgatgc aatacgtgta cgaggcggcg atgaggttca
     4021 cgcccagcgc ggcgagccgg gacgacatgc cgctgagctg gtgccacggg gcgatattgt
     4081 cccagagcag ccggtagcgc tcgtcgggga cgggatagcg cccgtcggcg atggcgccct
     4141 cggtctcgtc ggcgagcagg cggaaatgct ccgccagggc tggcgtcccg cgcgcggtga
     4201 ggatgggcgc catctgcacg aatgaatcga agacggtgat gcccgagggc cggtgcgcgg
     4261 cagcgcgcag aaatcgcttc cattcgcgca tggcgtcgca ggaatgccgc accgcctcgc
     4321 ggacgcgctc gatgtcgaag cgctgcccgc tcaagcgctc gatctcccgg atgagatcgc
     4381 ggtactgcgc cgcgatgtag tcggtatctc gatcggactg cgggccatag cagaagggaa
     4441 cgtcgacgat gaaatgcggt acgccccatt cgcgtcggta cacgtcgaac cacttgacca
     4501 ggagcgagca attgttgttg ttcgacacca gcaggtgcgg ccgcgggagc ccgaaggtgg
     4561 gcgagtcctt cccgccgtcg aaggccgttc cgatgtcgat gcgcgcatag gaacacaggt
     4621 cggccgaata gccgcgctcc tcggccttct ggcactgcac ggtccccacg cccttccccg
     4681 ccgagagcgc ggcgtggctc tccgggaccg cgtacgccac gttctcgaag cccgcgaaga
     4741 tctccgcggg aaccacgatg gcgatccata cgacgaatgc gccctcgttc gcgcgccggt
     4801 gcagatcgag gtacccgtcg gcgatcatcc tgctgagccg tctgcccgat tcggtcttcc
     4861 gccgcccgcc ccccgccgcg cgtccgtcca tcatcgcccg cccttgagaa gctcggcgaa
     4921 cgcctcgatg cgcgtcgcga aggcggcgca gttttcgtaa tcgtcgatcg ccatctcgag
     4981 acggagcgac gaaaccccgc gctcgcgcaa cagcccctcg atgtgcggaa actccaggga
     5041 ctcgtactcg cagaatttct cgccaatgaa gacgagcccc cgcgcgcccg attcctcgac
     5101 gagcgataga atcgtatcca cgcgccggtc cgccgtcgga agcagggtcg tgcagggatg
     5161 atgccgccgg tagaagtccg cgtaataccc gcccggatcg ccgtcctcag gcggctgata
     5221 cccgtaggag cgccgttgcg tggccacatc gttcgcgacc acgcgcagtc cgcagcgctc
     5281 gatcaggtct atcatcgcgg gcggcggcgg gagaatgccg cttaccacga tcggaatggt
     5341 cgcggaggca ttgatcgata cggcggcgca atcatcgacc gccgtgcgca gccgcttcac
     5401 ctgctcctcc acgcccacca gatagccgaa gcggttgatc cgcgaaaagg cgcgatacaa
     5461 cagccgccct tcgccgacca gcgcctcgag gcggcgcagc aacgcccgca tctcgccgta
     5521 gcggcgctgg ctctccagga agcgctcgga cgaaaagtgg acaccgaatg ccgcctcgca
     5581 ctcggcgacg agcgcatcca tgcgccgtgc gaggtattcg gatgcctcgg tacgttccgc
     5641 ggggatcgcg ggaatgtgca gctttatgat gggaagctca cggccctgcg ctcccagccc
     5701 gcgctcgatg atctccggca ggttgcgcag ggtgtcgcag gcattgtaca tcagcaggcc
     5761 gtcgaggatc gcgccgtagt cggaaaggac gatctcgacc aggtggcgcg cgaccgagca
     5821 ggcgaacggc tggaggtgcc gatccgacag cggcgtcgat ttcacgatgc ccttgagctc
     5881 ccagagcacg accggccgca ggcccatcgc atgcagaagc tccagcggcg gatagagcgg
     5941 gaagcaaccg acgaccggcg tgcccttctc cctgcaggcg cgtattgttt caatcacgga
     6001 atccatcgat ggtaatatga taccagaatg cggcgcgcct gtccaccgaa aaatcaaacc
     6061 gcagcgcttc gatccggcgc gtttaatgct tgattcgccc ggccaaccca tcgaaactgc
     6121 gcgccatgct cacgagaaga gaattcctgt cgaccctgac ccgcgcgtcg gccgccggcg
     6181 tgcttgcggg actcggcgcc ggcgcgtgcg cgcacgccgc tcgatcgccc cattacgctc
     6241 cggttgggcg gcgcatcacc catcgccgct tcacgctcag gatgaagggg acctggtcca
     6301 tcgcgcgcgg ttcaatggac tcgaaggata tcgtgatcgt cgagctcgag cgcgacggat
     6361 acattggccg cggcgaggcg gcgccgagct cgttttacgg cgaatcggcc gaatcctcga
     6421 cggccttcgt ccaagccgta acgccgctcc ttgagtccgc cgactggcgg gacatcgacg
     6481 cgttcgaaaa agcaatcgtg cgccgcggcg ctcccgatcg cggcggactg tgcgccgtca
     6541 ccacggccct gctcgattgg aacgctcgtc ggcgcggcct ccccctgcac cggcacctgg
     6601 gcatcgacgc gcgcaccatg ccgcgatcga cgttttccat cggcatctcc gcgcgcggcg
     6661 agatgctccg caaggtgcgc gccgcctcgg cctatccgct catcaagatc aaggttggct
     6721 tcgacggcga tatcgccatc atcgaagcga tacgctccat cagccgacag acgatccgcg
     6781 tggacgcgaa cggcggctgg aggtcaaaag aggaggcgct ggaaaagatc acggcgctcg
     6841 cgcggctggg cgtggagttc gtggaacagc cgctccccgt ggaccgcatc gacgatgcgc
     6901 gctggcttac cgcgcgttcg ccccttccca taatcgccga cgagggcgtc cggaccgtgc
     6961 gcgacatcgc gccgctcacc ggggcgtgcc acggcatcaa cataaaactg atgaagtgcg
     7021 gcggcatccc cgaggcgctg cggatgatcg cgctcgctcg gagccacggc atgcgagtga
     7081 tgatgggatg catgatcgaa agctcgctgg gcatcaccgc cgcggcgcac atcgcgccct
     7141 tggtcgactt cgccgatctc gacggaaacc tcctggtggc gaacgacccg ttcgcccgcg
     7201 gcgttcgaat cacaaacggc ctcctggtcc tccccggcgg gccgggcatt ggcgtggagc
     7261 aggccccttt cacggcctga tacgcactgc accatgggtg caagcggcgc tgtcgctttt
     7321 cgcacctcct tttttacgac acgtcctttc cgtgtttgga ttagcaccag gaaagtgcct
     7381 gatattatgt aaattcgggg aggctgtgcc ttttggcata agcggcccct ttggtcgcac
     7441 gctgcggatg ggcctccgcg ccctccacga ggcggcgaat gcgcgtacac ggcgccccgc
     7501 ggatggcttg cccgtcctcg cgcgcatccg agcgcccgca cctccttgtg cgggcgtctg
     7561 gttgcgccag tacggtaatc gtcagcggga gagcgtttac aatgatccga tcccgcattc
     7621 tgatggctgc catccctgag agcgtgttga caactgcgcg cgccgccggt accatgtccc
     7681 cggcaacaac gccccgacgg gaggatccat gcacttcgac accgatacgc gaaccgaaag
     7741 aacgtcaccg ggcgtctaca gcgccgccat aagcgaccgg tggtccatca acaacaatcc
     7801 caacggcggc tatctcatgg ccgtcatgtc acgggccctg cttacccaaa gcgacttctc
     7861 gtccacgccc atcgtcacgg caaattactt ggcgcgctgc gtccccgggc cggcaaccat
     7921 cgaggtccgc gagatatcga ggaccaggca gttccatcgc ttcgaggcgt ccatcatcca
     7981 ggacggtgcg gagcgggtgc gtgcgctcgc caccttcgcg tccggcggcg gcgactgctc
     8041 catcaaccgc tacgaggcat cgcccccgga gctcgcgccg cgtgaagatt gcgtcctcat
     8101 ccccactatc ccgacataca cattgttcgg cgagctcgac gtcatgctgg acccgtcctg
     8161 cgccggctgg atgcaaaaca aacatgtgga acgctccgag aacaagggct ggatgcgctt
     8221 caagaacgag cgcccctacg atctgcccgc ggtgctgctc attgcggac
//
LOCUS       DAORHF010000166         8254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8204_length_8254_cov_4.704720, whole genome shotgun sequence.
ACCESSION   DAORHF010000166 DAORHF010000000
VERSION     DAORHF010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8254
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8204_length_8254_cov_4.704720"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PKJ16_12945"
     CDS             complement(<1..771)
                     /locus_tag="PKJ16_12945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV47943.1"
                     /translation="MKKAVCIGIMLMIAAAMGCKREGDILATYQGGDIKRGDLYEWLK
                     ERHFQKDSVVKTKKAQKEKLERIAIERIAALEAKKAGFDATDEFKAMADMAMEHQLTK
                     LLYQKEIRDTAKFKEPIVRISHIYFKIRDARVEGAKREKLSEKDLAAELEAATQKAQG
                     VIEQLKKGEKFDELAKKHSDDFSKKNGGDIGFRIAEMLPDNYAAAAFALKEDAYTVTP
                     VRVYSEQPGKMPNGVYVIRVTAKKTLTESNVEDIIDNKV"
     gene            complement(882..2981)
                     /locus_tag="PKJ16_12950"
     CDS             complement(882..2981)
                     /locus_tag="PKJ16_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV47944.1"
                     /translation="MHTIFYPESIVIIGLSSKPNNIPRMILENLLRWGYRGRIFGMNP
                     RAEDAHVDGIKMYRAMEDLPEVPDLAVCLIPARFVPEMIEVCGKFGIPRMAIPSGGFA
                     EFGEEGKKLADAALANARRYNIRFVGPNGVTVANTANGLCLPFVPLYRPPKGGLSVIS
                     QSGGVGLMMWNLLMEENIGMAKFASIGNKLDLDEVDFLRYFGDDPDTSIICLYLESVV
                     RGRDMVEAAKTINKPIVVFKSNTTEIGKKAAMSHTAALSNDEDIIDSAFESAGIIRIR
                     NYTDFVAVAKAFELPPMRGNRIMVMSPAGGFSVITADLCENAGFSFADPGEEFYSGLQ
                     QFANAGVIKFSNPLDMGDIYDPQLSAHVMYSVMHNEKVDGAVYVSQRPYMPSGTDVFS
                     RMFLTDLSKETWGAILSSGKPLGICLFGLSRTLSQVKQNVNFPIFNTPEEMVRALAAQ
                     RDFVNRALSADVPEGPPPGADIAAADAWIAAHPGDRGEEALELLSAFGVRVAESGVAE
                     NADAAVELARRIGYPVVMKVVSPDALHKSDAGGVAVNVADDDSVRKNFSTIRDNLRTY
                     NKNARFDGVRVQWMAGPGFDMFIGGKFDESFGQVVVFGYGGIYVEAFRDVATLLCPAA
                     PDEIARRLSRLKSHRLLAGARGGARTDIEGLVDAVHRVSCLLAREPRIRELDVNPLRV
                     LADGSGVMALDARLRIV"
     gene            3143..3679
                     /locus_tag="PKJ16_12955"
     CDS             3143..3679
                     /locus_tag="PKJ16_12955"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV47945.1"
                     /translation="MKRYHDRLKNILSSEELCCIKKDSSTLIEIAYDYIEMGEFKKAF
                     RLFCVGARLNSLDPDVLNGLGVSLCELGKFDISRQILENAIRLYPDNPVILSNLASIY
                     RETGLIELAIHCYHRAVTIDPGIVEAHLNLADLYYDTGDLFVAYLTVLEVLKNHPGNE
                     EAIELRDDLLINLGLSIA"
     gene            3813..4091
                     /locus_tag="PKJ16_12960"
     CDS             3813..4091
                     /locus_tag="PKJ16_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47946.1"
                     /translation="MVKEKIFCANCQYCIVIRQYESEPDKYVLRVKCMKKKWVKRSGE
                     EKFYKYFTVARRIMDDCDSYSPVGEAYPYIKNLRKELPIKDEIYSTKE"
     gene            4094..5419
                     /locus_tag="PKJ16_12965"
     CDS             4094..5419
                     /locus_tag="PKJ16_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HNV47947.1"
                     /translation="MRAGSFSGGIRPPETKGRIENSPFSNLSVPHQCSIPIQHRAGTA
                     ARPVVAVGDVVREGQLIAADEGAGDIRVHASIPGRVMDIGDRPAHSGGCAPCIVIEAA
                     GSFSGFGAAAPCDWTTLTPVEIVRRVREAGVMCAHGAGIPAARILAPPEGCAIDTIIV
                     HGAECEPYLTAADMLLRAYPAEILEGARIALRALGASRAIIAIPKSLTTALAAIRTAL
                     AAHAGPGTVTVRPVSDKYPQGNERLIIKAAARKTIHAGTDLTRAGFAVLNAATVIALR
                     EAVLAEKPLIERPITVGGPGIRKPGNYKVRIGTAVEDVIRECEGLVDPAARVIMGGPI
                     SGTAVRDMRVPVTGTTTAIIALLGPDHGASGERECIRCGRCVAACPMGLMPHELDEAA
                     RQDRHDAREARGVEACIACHACSYVCPSRRPIGRRIDAARTNRQGAIDG"
     gene            5412..6374
                     /locus_tag="PKJ16_12970"
     CDS             5412..6374
                     /locus_tag="PKJ16_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HNV47948.1"
                     /translation="MANGTAPAHTPCPPHMHDSRSLAGMMRTVCIALAPALAAGAALL
                     GAGILVNAAAAVLAAIASEALFLLAARKKITLTDGSAAVTGLLVALNLPAGTPPWLAA
                     AGGAFAIVFIKQLSGGMGANPFNPALGALAIMHLFFPAEMGGAWHSFSTAPPITAETQ
                     SLAPFVALLTGGSGATVAEVAAPLALCGGIFLLVRRVISWHIPIAFLAAAGATAFFLF
                     RATGTGESAAALSAGYHLVAGGLFLYAFFMATDPATSPVTAPGMVLFGACCGALVIIV
                     RPFGGYPETAPLAMLLMNALAPLIDRITRPRMFGGRAAALRQKN"
     gene            6439..6798
                     /locus_tag="PKJ16_12975"
     CDS             6439..6798
                     /locus_tag="PKJ16_12975"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV47949.1"
                     /translation="MKQYFDYTLQGSIAVFTLRIDRLLFFESAQILATVEQKLGELGF
                     PDLIIDLRSVGTLDSAGVGFLISVKHLIDTHQRRIAVVCDNESVLRVLSITNMESFLR
                     VCNSLEQAHASFGEGSA"
     gene            6795..7307
                     /locus_tag="PKJ16_12980"
     CDS             6795..7307
                     /locus_tag="PKJ16_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV47950.1"
                     /translation="MSVVLEAIYTRRSIRRYAPDPVPEDAIREIVRAGMNAPSAGNEQ
                     PWQFVIIDRREILDGIPTVHPHAAMIREAPVAILVCGDLTLETKQGYWVQDCAAATQN
                     MLLAAAALGLGSVWLGVYPRDDRVTGLRRLLGIPETVVPFALLPVGHPAEHKPRKDEF
                     HPERIHRNAW"
     gene            complement(7343..7534)
                     /locus_tag="PKJ16_12985"
     CDS             complement(7343..7534)
                     /locus_tag="PKJ16_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47951.1"
                     /translation="MSPVGTALFTLLFVAVMVLLGGFYTTLRLSQTAMIAVTAVTAVV
                     FVANLVREQFFMKKEEDSD"
     gene            8067..>8254
                     /locus_tag="PKJ16_12990"
     CDS             8067..>8254
                     /locus_tag="PKJ16_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47952.1"
                     /translation="MKKVLVIFVAVMMATMFVNFADAKKKKGAPVDKECVKKCAAEVK
                     SCNAEVKKLKGKEKRAKA"
BASE COUNT         1494 a         2706 c         2567 g         1487 t
ORIGIN      
        1 gaccttgttg tcgatgatgt cctcgacatt gctctcggtg agggtcttct tcgcggtcac
       61 cctgatcaca tagacgccgt tgggcatctt cccgggctgt tcgctataca cgcgcacggg
      121 cgtcacggtg tacgcgtcct ccttgagcgc gaaggcggcc gcggcatagt tgtccggaag
      181 catctccgcg atgcggaagc cgatatcgcc gccgttcttc ttcgaaaaat cgtcggaatg
      241 cttcttcgcg agctcgtcga atttctcgcc cttcttgagc tgttcgatga cgccctgcgc
      301 cttctgcgtg gcggcctcga gctcggcggc gaggtccttc tcggagagct tctcgcgctt
      361 ggcgccctcg acccgcgcgt cccggatctt gaagtagatg tggctgatgc gcacgatggg
      421 ctccttgaac ttcgcggtgt cgcggatctc cttctggtag aggagcttgg tgagctggtg
      481 ctccatcgcc atgtcggcca tcgccttgaa ctcgtcggtc gcgtcgaaac cggccttctt
      541 ggcctccagt gccgcgatgc gctcgatggc gatgcgctcg agcttttcct tttgcgcctt
      601 cttggtcttg acgaccgagt ccttctggaa gtggcgctcc ttgagccatt cgtacaaatc
      661 accgcgcttg atatcgccgc cctgataggt ggcgaggatg tcgccctcgc gcttgcagcc
      721 catggccgcc gcgatcatca gcatgatccc tatgcatacc gccttcttca ttgcgaaaca
      781 ctcctttatg gtcagattct tccgtgcgtc cggggtgggt ccgccgtcgt tgtatactcc
      841 cgctatatcc cggccgcggt cggccctgtc aacccgaatt tctacacgat gcgcagacgc
      901 gcgtccagcg ccatgacgcc cgagccgtcg gccaggacgc gcagcgggtt cacatcgagc
      961 tcgcgtatcc ggggctcgcg cgcaagcagg cacgataccc ggtgtacggc gtcgacgagc
     1021 ccctcgatgt ccgtgcgcgc cccgccgcgc gcgcccgcga gcagccggtg cgatttgagg
     1081 cgcgagaggc gccgcgcgat ctcatcgggc gccgcggggc acagcagcgt cgcgacatcg
     1141 cggaacgcct ccacgtagat gccgccatag ccgaacacga ccacctggcc gaacgattca
     1201 tcgaacttcc cgccgatgaa catgtcaaag cccgggcccg ccatccactg cacccgcacg
     1261 ccgtcgaaac gcgcgttctt attataagta cgaaggttgt cgcgtatcgt tgaaaagttt
     1321 tttcgcacgg aatcgtcatc ggccacattc acggcaacgc cgccggcgtc cgacttgtgg
     1381 agcgcgtccg gcgagacgac cttcatgacg accggatagc cgattcggcg cgcgagctcg
     1441 acggcggcat cagcgttttc ggcgacgccg gattcagcga cgcgcacgcc gaacgcggac
     1501 aacagctcca gcgcctcctc gccgcggtcg cccgggtgcg cggcgatcca cgcgtcggcg
     1561 gccgcgatat cggcgccggg cggcggaccc tccggaacgt cggcggagag cgcgcggttg
     1621 acgaaatcac gctgcgcggc gagggcgcgc accatctcct ccggcgtatt gaatatgggg
     1681 aagttcacgt tttgcttcac ctgcgaaagt gtccgcgaca ggccaaagag gcagatcccg
     1741 agcggttttc ccgacgagag gatcgcgccc cacgtttcct tcgaaaggtc ggtgaggaac
     1801 atgcgcgaga agacgtccgt gcccgagggc atgtacggac gctggctcac gtagaccgcg
     1861 ccgtcgacct tttcgttgtg catcacgctg tacatcacgt gcgccgagag ctgcggatcg
     1921 tagatatcgc ccatatccag ggggttcgag aacttgatca cgccggcgtt cgcgaactgc
     1981 tgcagcccgc tgtagaactc ctcgccggga tcggcgaagg aaaagcccgc gttctcgcaa
     2041 aggtcggcgg tgatgaccga aaacccgccg gccgggctca tcaccatgat gcggttgccg
     2101 cgcatgggcg ggagctcgaa ggccttcgcg acggccacga aatcggtgta gttgcggatg
     2161 cggatgatcc cggcgctctc gaaggcggaa tcgatgatgt cctcgtcgtt ggacaacgcc
     2221 gccgtgtggc tcatcgccgc cttcttgccg atctccgtgg tgttggactt gaagaccacg
     2281 atgggcttgt tgatggtttt ggccgcctcc accatgtccc ggccgcgcac cacgctctcc
     2341 agatacaggc agatgatgga cgtgtcggga tcgtcgccga agtagcggag gaagtccacc
     2401 tcgtccagat cgagcttgtt gccgatgctg gcgaatttcg ccataccgat gttctcctcc
     2461 atgaggaggt tccacatcat gagccccacg ccgccgcttt gcgaaatgac cgacagcccg
     2521 cccttcggcg ggcggtagag cgggacgaag ggcaggcaca ggccgttcgc ggtgttggcg
     2581 acggtcacgc cgtttggccc cacgaagcgg atattgtagc gccgggcgtt ggcgagcgcg
     2641 gcatcggcga gctttttccc ctcctcgccg aactccgcga aaccgccgga tgggatcgcc
     2701 atgcgcggga tcccgaactt cccgcacacc tcgatcatct ccggcacgaa acgcgcgggg
     2761 atgaggcaga ccgcgagatc gggcacctcc gggaggtcct ccatcgcgcg gtacatcttg
     2821 atgccgtcga cgtgcgcgtc ctccgcgcgc gggttcatgc cgaatatgcg gccgcgataa
     2881 ccccagcgca ggaggttctc gagaatcatc cgcggaatat tgttgggttt ggacgagagc
     2941 ccgatgatga cgatggactc gggatagaat atcgtgtgca tggcgtcctc cgttttgtgt
     3001 gtgcggtgcg cgcgaaccgg acggaattgt cgcacgcgac aaccgtcggg gcaacgaaaa
     3061 attattttgt tgaaaaaaat taattcatcg ggacaatgac cgaagcaggt agaacgcgcg
     3121 accgcctcct atgaaaaaag acatgaaacg ataccacgac cgcctgaaga atatcctctc
     3181 gtcggaggag ctctgctgca tcaagaagga ttcgtcgacg ctcatcgaga tcgcctacga
     3241 ttacatcgag atgggcgagt tcaagaaggc cttccggctc ttctgcgtgg gcgcgcgcct
     3301 caacagcctc gatcccgatg ttttaaacgg gctcggcgtg tccctctgcg aactggggaa
     3361 attcgacatc tcgcgccaga tcctcgagaa cgcgattcgc ctctacccgg acaaccccgt
     3421 gatcctgtcg aacctggcga gcatctaccg cgagacgggc ctcatcgagc tggccatcca
     3481 ttgctatcac cgggccgtca ccatcgatcc cggcatcgtc gaggcgcacc tcaacctggc
     3541 cgacctctac tacgacaccg gcgatctttt cgtggcctac ctcaccgtgc tggaggtgct
     3601 caagaaccat cccggcaacg aagaggccat cgagctccgg gacgacctcc tgatcaacct
     3661 gggcctctcg atcgcctgag caatcgaaaa attccttgac caaaaacgcg tccttcgcct
     3721 aatggacatt tacaaaacca tacggcccgc atgcgtacgc cgccgctacc cgcacggccg
     3781 ggaaacattg agacatcccc gaggagcaac gaatggtcaa ggaaaagata ttctgtgcaa
     3841 attgccagta ttgcatcgtg atccggcagt acgaatcgga gcccgacaaa tacgtcctgc
     3901 gcgttaagtg catgaagaaa aaatgggtca aacgctccgg cgaggaaaag ttctacaaat
     3961 acttcaccgt cgcccgccgg atcatggacg actgcgattc ctacagcccc gtgggcgagg
     4021 cctacccgta cataaagaac ctccggaagg agctccccat caaggacgag atctattcga
     4081 ccaaggaata ggcatgcgcg ccggttcctt ttcagggggg attcgccccc cggagacgaa
     4141 gggccgtatc gaaaacagcc cgttttccaa tctcagcgtt ccccatcagt gcagcatccc
     4201 gatccagcat cgcgccggaa ccgcggcgcg gccggtcgtg gccgttggcg acgtggtccg
     4261 cgagggccag ctgatcgccg ccgatgaggg cgcgggagat atccgcgtgc acgcgtccat
     4321 ccccggccgg gtgatggaca tcggcgaccg ccccgcgcat tcgggcgggt gcgcgccctg
     4381 catcgtcatc gaggccgcgg gctcattctc cggatttggg gccgccgcgc cctgcgactg
     4441 gacaacgctc acgcccgtgg agatcgtacg ccgcgtacgc gaggccggcg tgatgtgcgc
     4501 ccacggcgcg ggcatcccgg ccgcccggat cctcgcgccc ccggagggtt gcgcgatcga
     4561 cacgattatc gtacacggcg ccgagtgcga gccctacctc accgcggccg acatgctgct
     4621 gcgagcctat ccggccgaga tcctcgaggg ggcgcggatc gccctacggg ccctcggcgc
     4681 gtcgcgcgcg atcatcgcca taccgaaatc gcttacaacg gccctcgcgg cgatccgcac
     4741 ggcgctcgcc gcccacgcgg gccccgggac tgttaccgtg aggccggtct cggacaaata
     4801 cccgcagggg aacgagcggc tgatcatcaa ggccgccgcg cgtaagacga ttcatgcagg
     4861 gaccgacctc acgcgcgccg ggttcgcggt actcaacgcg gcgacggtca tcgcgttgcg
     4921 cgaggcggtg ctggccgaga agcccctcat cgagcggccc atcaccgtgg gcggtccggg
     4981 catcaggaaa ccgggcaatt ataaagtacg catcggcacc gccgtcgagg acgtcatcag
     5041 ggaatgcgag gggctggtcg atcccgcggc gcgcgtcatc atgggcggcc cgatctccgg
     5101 gaccgccgtt cgcgacatgc gcgtccccgt gaccggcacg acgacggcta tcatcgccct
     5161 cctcgggccg gaccacggcg cgtcgggcga gcgcgagtgc atccgctgcg ggcgctgtgt
     5221 cgcggcctgt cccatgggac tcatgcccca tgagctggac gaggcggcgc ggcaggatcg
     5281 ccatgatgcg cgtgaagcgc gcggcgtcga ggcgtgcatc gcctgtcacg cctgctcgta
     5341 cgtatgcccg tcgcgtcggc cgatcggccg tagaatcgat gcggcgcgca ccaaccgtca
     5401 gggggccatc gatggctaac ggcaccgcac cggcgcacac gccctgcccg ccccacatgc
     5461 atgattcccg gtcgcttgcg gggatgatgc ggaccgtctg catcgcgctc gcgcccgccc
     5521 tcgccgccgg cgccgcgctc ctgggcgcgg ggatactcgt gaacgccgcc gcggcggtgc
     5581 ttgcggcgat agcctccgag gcgctctttc tcctcgcggc gcggaaaaag atcaccctga
     5641 ccgacggctc ggcggccgtg acgggactcc tggtggcgct caatctgccg gccggaacgc
     5701 ccccctggct cgctgcagcg ggcggcgcat tcgccatcgt attcatcaag cagctatcgg
     5761 gcgggatggg ggccaatccg ttcaatcccg cactcggggc gctcgcaatc atgcacctgt
     5821 tttttccggc ggagatgggc ggcgcctggc attctttttc gaccgcacct cccatcaccg
     5881 ctgagacgca gtcccttgcg ccatttgtcg cgctcctgac cggcggctcg ggggccaccg
     5941 tcgccgaggt tgccgcgccg ctcgcgctct gcggggggat cttcctgctc gtccgcaggg
     6001 tgatctcgtg gcacatcccg atcgcgttcc tcgccgccgc gggagcgacc gcgttttttc
     6061 tcttccgcgc cacgggaacg ggagagagcg ccgcggccct gtccgccggg tatcacctgg
     6121 ttgcgggggg actgttcctg tacgcgttct tcatggcgac cgaccccgcg acatcgccgg
     6181 taacggcgcc tggcatggtc ctgttcgggg cgtgctgcgg ggcgctggtc atcatcgtcc
     6241 gtccgttcgg cggctacccg gaaacggcgc cgctcgcgat gctcctgatg aacgcgctcg
     6301 cgccgctcat cgaccgcatc acccgtcccc gcatgttcgg cggtcgcgcc gccgcactgc
     6361 gccaaaagaa ttgacgaaat cgacgcaacc cgctatacta ccggaaacgg cgcggcgtac
     6421 ggcgccgcat ccatcgacat gaaacagtat ttcgactaca ccctgcaggg ctcgatcgcc
     6481 gtcttcaccc tgcgcatcga ccggctgctc tttttcgagt cggcgcaaat cctggcgacg
     6541 gtcgagcaga agctgggcga gctcggcttt cccgacctga tcatcgacct gcgctcggtg
     6601 ggcaccctcg attccgccgg cgtgggcttc ctcatctccg tgaagcacct catcgacacg
     6661 caccagcgcc gaatcgcggt cgtgtgcgac aacgagtcgg tcctccgggt cctgagcatc
     6721 accaacatgg agagcttcct gcgcgtgtgc aatagcctcg aacaggccca cgcctcgttc
     6781 ggcgagggat cggcatgagc gtggttctgg aggcgatcta tacgcgcagg agcatccggc
     6841 gctacgcccc ggacccggtc ccggaggacg ccattcgcga gatcgtacgg gccggcatga
     6901 acgccccgtc ggcgggaaac gaacagccct ggcagttcgt gatcatcgac cgccgcgaga
     6961 ttctggacgg cataccgacc gtgcacccgc acgctgcgat gatccgcgag gcgcccgtcg
     7021 cgatcctcgt ctgcggcgac ctgacgctgg agacgaagca gggctattgg gtgcaggact
     7081 gcgccgccgc gacacagaac atgctgctgg ccgcggcggc cctgggactg ggctcggtat
     7141 ggctgggggt ctatccccgc gacgaccgcg tgacgggact gcggcgactg ctcgggatcc
     7201 ccgaaaccgt ggttcccttc gccctccttc ccgtcggcca tcccgcggag cacaagccgc
     7261 gcaaggacga gtttcacccc gagcgcatcc accgaaacgc ctggtaaggc gccggggcgg
     7321 cggaccgccc cggcatgcgg catcagtcgc tatcttcctc ttttttcatg aagaattgct
     7381 cacgcaccag gtttgcgaca aagacgacgg ccgtaaccgc ggtgacggca atcatcgccg
     7441 tttgggacaa acgcagggtc gtgtaaaagc cgcccagcag aaccatgacg gcgacaaaca
     7501 gcagggtaaa caaggcagta ccaactggac tcatattctt ctctccttcg tgtggtgatc
     7561 cgccgcgcgc tgcgcggcgc actctgcgga tgatcgctcc cgaccgggag cggtgtcgga
     7621 gaggctagcg gaaggtcccc gaactccatc ggggatattt gttctgatga catgggtcgg
     7681 catcggcatg acgggccgat tctccgaaac ggacggcccg tacccgcgga agcggatgat
     7741 gtgcgagcgc gaaatgctgg gggtgcttct tgggctgcgg ggtggccgtc cgggcaagcg
     7801 catcggcgat ccccgggtcg gccaggcggc aatcgaccga cccaacggcg cttgccgcgc
     7861 ccggcgatat aaacgcatag gcgatacacg cggcaatccc gacgagacag actgcccgcc
     7921 gccaacgtgc gcatgatgac cggctttttc ccattacgga gccaattcta tcacatgggg
     7981 aaaaaatagc ttgcaaaatt ccaaaacatc ctatttttgc cgaacaactc atcgagcaga
     8041 tgggttaatt atttccggag ggtactatga agaaagtact ggtcattttc gttgccgtta
     8101 tgatggcaac catgttcgta aatttcgccg atgcgaagaa gaagaaaggc gcccccgtcg
     8161 acaaagagtg cgtcaagaag tgcgccgccg aagtaaaatc ctgcaacgcc gaagtgaaga
     8221 agctgaaggg caaggaaaag agggcgaagg cgaa
//
LOCUS       DAORHF010000167         8233 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8239_length_8233_cov_4.687087, whole genome shotgun sequence.
ACCESSION   DAORHF010000167 DAORHF010000000
VERSION     DAORHF010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8239_length_8233_cov_4.687087"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PKJ16_12995"
     CDS             <1..274
                     /locus_tag="PKJ16_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47953.1"
                     /translation="FTGSLTEMNSEIQQKINKQSVISNSIYTFSMEQKHVIDELTRAI
                     ESINEISQKTLESAEMVKSYSKIIDMSANELSANIEAFKKLEQKVK"
     gene            complement(313..1185)
                     /locus_tag="PKJ16_13000"
     CDS             complement(313..1185)
                     /locus_tag="PKJ16_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47954.1"
                     /translation="MTATMRFEGAVSDGARRTFRFASYVRPEFIERALLVAFEDGRIV
                     ELYFSSNAYGTARWTEQSGLVDSHRWESEDEARSGSYLARRYPIILENGPVSDAYALR
                     AIVPDVLDALVDRALAGYLMTLVSGFRIVDADDFETRQRRLDEECVAVQGPPAAIGGT
                     LRPVPSMAVRDDGAGDVVTRPPSHMRESVPTAQTEADPIGQQEEPVGRPRHERPIDDA
                     AISADAAWSGDALAVREGRMGSIDRLREFAAGVREFFFPAVRNNERSAGTDGGTARER
                     MDDAARIAKRIKRR"
     gene            1347..2618
                     /gene="murA"
                     /locus_tag="PKJ16_13005"
     CDS             1347..2618
                     /gene="murA"
                     /locus_tag="PKJ16_13005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HNV47955.1"
                     /translation="MDIYKITGGATLQGEAPISGAKNAALPLIVASLLAEGETVLTNV
                     PNLQDVRTMIKVVQCLGAQTDFDTDANVLKIRVAALTNAEVPYDLVKTMRASVYVMGP
                     LLARAGEADVSLPGGCAIGERPIDIHLSGFEAMGATIELEHGYIKARAERLSGGRFVM
                     RNVSVGATANLLMGAVLADGETVLENCAKEPDIVDLGRFLVKMGATIEGLGTDRIIVQ
                     GVPKLSAVTYRVMPDRIEAGTYLLAGAITRGSVKIVRAEPDHIGALIQTLGEMGFAIE
                     TGSDWVSIAPGDRALSGAFIRTLPYPGFPTDLQAPMMSLMTTAPGISVIIETIFENRF
                     THVGELRRMGADITTEGNVAIIKGQERLSSATIMMSDLRAGAALVLAALAAQGTTEIR
                     RIYHSDRGYEKLTEKLLGLGADIKREAGGKL"
     gene            2618..3454
                     /gene="flgF"
                     /locus_tag="PKJ16_13010"
     CDS             2618..3454
                     /gene="flgF"
                     /locus_tag="PKJ16_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HNV47956.1"
                     /translation="MLRGIYTGASGMIAQQARMDAVANNLANVDQTGYKKDTAIFKTF
                     PEMLIRRLSDDGLGIVPTGSYDTMPFVGKLGTGVEVNEVYTQFEQGSLQRTENSFDLA
                     LEGRGFFAVQTERGERYTRNGAFTINQDGVLMTHNGDPVLGENGVIRVQQNNFIVNER
                     GEIIVNQALSLDPRDPVNMTGNTWESPTVIDRIKLIDFERIRELKKEGNSFYRETPES
                     GPPLPPAEIKVVQGFLEKSNVSAVREMVDMIEVQRSYEANQKTIQTHDQTVGKLINDI
                     VR"
     gene            complement(3458..4675)
                     /gene="nifS"
                     /locus_tag="PKJ16_13015"
     CDS             complement(3458..4675)
                     /gene="nifS"
                     /locus_tag="PKJ16_13015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HNV47957.1"
                     /translation="MRHGHQRQSREEWVYLDNNATTKVDPEVIEAMMPFLRERYGNPS
                     SMHDFGGGVATHLERARAQVAELIGAENDYEIVFTSCGTESDNMAILGAISYYTEKRH
                     IITTRVEHPAVLNLCKKLERDGYRVTYVPVNRAGELDLEHLKASVDDDTAIVSVMHAN
                     NETGVIFPVEEIGAFLRERGVPFHIDAVQAAGKVPINVKRIQCDLLSLSGHKFHAPKG
                     VGVLYVRRGTRMRPIMYGGHQERGRRPGTDNVAGIVAIGKAAELARIYLNREHEIAAM
                     RDRLEHEILERFPNASLNGSRDARVPNTTNVGFEFIEGEAILLYLNEKRIAASSGSAC
                     SSGSLEPSHVLRAMGVPFTSAHGSIRFSLSRFTTDEEIDYTLEVLPGIIDRLLEISPF
                     WDNEKKCGRPVTV"
     gene            complement(4718..5560)
                     /gene="nifU"
                     /locus_tag="PKJ16_13020"
     CDS             complement(4718..5560)
                     /gene="nifU"
                     /locus_tag="PKJ16_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein NifU"
                     /protein_id="HNV47958.1"
                     /translation="MWDYTDTVKDLYTNPRNVGRIDDANAVGEVGSIVCGDALTLYLK
                     IENDVIVDAKFQTFGCGSAIASSSALTEMIKGKTVAEAETITNKDIVDFLGGLPEQKM
                     HCSVMGREALDKALANWRGVSIEEHADTDGAIVCQCFGITDTLIRKVIRENNLKTVED
                     VTNYTKAGGACGSCIPEIEHIIREELRQHQAEPAPEEHPRALTNIRRIQLVEETINNI
                     IRPILQRDGGNIDLVDIEGKRVIVALRGACASCRLSDVTLKNLVEDKLREFVEDDIVV
                     VEAK"
     gene            6063..7493
                     /locus_tag="PKJ16_13025"
     CDS             6063..7493
                     /locus_tag="PKJ16_13025"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HNV47959.1"
                     /translation="MVKLVHNDLLGMIRDSKTAPDAGLQDMPGASVRFDDILARQLHE
                     TNKPSEPESRRESALQPREDARPPDSPAETAHHPAPRASEDDSRVRDSEANHEPETKE
                     QNAISEKADSAQRNGDDSDTGEQSDEDHRTHRGRTIDPLLMDLVNPRATDAKPGGNAA
                     IQDLVEKTRAALDAIIKKGASKTGDARDAALMNTLRELGRELEALSGNASPQGHRQQL
                     LALLQKIKTNISTLDGNAAHVADRELGQLIQGLKKHIGEIMTALSKSDKGAAQDFAST
                     MNQRRESGEPRPATERAFVPQTAASIDAVEHRPSGNNDSALGFQFSKHGAQTMRMGEP
                     GQPAPRAGLFGEQLQQVMENAKVFIRDSRNGSFSLQMHPESLGKVNVSLGLDHGVLSG
                     RFLVESNEARDLLLENIDQLKEKLAEAGVSVGEFNVNVRDHREHRFDEREQETEFIHR
                     GMPREAGAGYDAPARSRHDGAIDMVI"
     gene            7503..8051
                     /locus_tag="PKJ16_13030"
     CDS             7503..8051
                     /locus_tag="PKJ16_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008882406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HNV47960.1"
                     /translation="MDKLTKMDSRDLMRTQQRVDEINKKFRKPDGKLKGELGKDSFLK
                     LLVTELRHQDPTNPMADKEFISQMAQFSSLEQMHNLNNSMKGLTDMAQSNKAYSLLGK
                     SVEAVDPKTGKVVRGAVTGITHNGNDIRIKIGATEVGLAEIHGVYENGPAEKKNAAPD
                     APPAIKRNEPAGAHQIKAYQLF"
     gene            8104..>8233
                     /locus_tag="PKJ16_13035"
     CDS             8104..>8233
                     /locus_tag="PKJ16_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HNV47961.1"
                     /translation="MMRSLFSGVSGLKNHQTRMDVIGHNISNVNTYGYKYTRATFQD"
BASE COUNT         1724 a         2711 c         2449 g         1349 t
ORIGIN      
        1 gttcaccggc agcctcaccg agatgaacag cgagatccag cagaagatca acaagcagag
       61 cgtgatctcc aacagcatct acaccttctc gatggagcag aagcacgtga tcgacgagct
      121 cacccgggcc atcgagtcta tcaacgagat atcgcagaag acgctggaga gcgccgagat
      181 ggtgaagagc tactcgaaga tcatcgacat gagcgcgaac gagctctcgg cgaacatcga
      241 ggccttcaag aagctggaac agaaagtcaa gtgacgatgc ggcgcgccgc gccgaactcg
      301 ccctcagccg cgctaccgtc gcttgatccg cttggcgatg cgcgcggcgt catccatccg
      361 ttcacgcgcc gtaccgccgt ccgtccccgc cgagcgctcg ttattgcgca ccgcaggaaa
      421 gaagaactcc cgaacgccgg cggcaaactc tcgcaatcgg tcgattgagc ccatgcggcc
      481 ctcgcggacc gcaagcgcat ccccgctcca cgctgcatca gccgatatcg cggcatcgtc
      541 gatcgggcgt tcatgtcgcg gccggccgac gggctcctcc tgctgcccaa tgggatcagc
      601 ctccgtttgc gccgttggga ccgactctcg catgtgtgac ggcggccgcg tcacgacgtc
      661 accggcgcca tcatcgcgca ccgccatgga cgggaccggc ctcagggtcc cccctatcgc
      721 ggccggggga ccctgcacgg cgacacactc ctcatccaac cgccgctgcc gcgtttcgaa
      781 atcgtccgca tcgacaatgc gaaatccgct caccagcgtc atgagatacc ccgccagcgc
      841 gcggtcgacg agggcgtcga gcacgtcggg aacgatggcg cgcagggcat acgcatcgct
      901 cacgggcccg ttctcgagga tgatcgggta gcgccgcgcg agatacgatc ccgagcgcgc
      961 ctcgtcctcg ctctcccagc ggtgcgaatc gaccaggccg ctttgttcgg tccagcgggc
     1021 cgtcccgtac gcgttcgacg agaaatagag ctcgacgatc cgcccgtcct cgaaggccac
     1081 cagaagcgcc cgctcgatga attccgggcg gacatacgag gcgaagcgga aggtccggcg
     1141 cgccccgtcc gataccgcgc cctcgaaccg catcgttgcc gtcatgccgt cactcatgcg
     1201 cactatgata tcgcggcgcg ccgcgaccgt caacgaaata tacccgcacc acgcgcaaat
     1261 acatttgaca aatgcacttc gcggatgcct gaggtatcag gacagcacac gacacccgcg
     1321 cggccgcgca caatcggggg acctacatgg atatctacaa aatcaccggc ggagcgacat
     1381 tacagggcga ggcgccgata tcgggcgcga agaacgcggc gctcccgctc atcgtggcaa
     1441 gcctcctcgc cgagggcgag acggtcctca ccaatgtccc caacctacag gacgtgcgca
     1501 ccatgatcaa ggtggtgcag tgcctggggg cccagacgga cttcgacacc gacgccaacg
     1561 tactgaagat ccgcgtggcc gcgctcacga acgccgaggt gccttacgac ctcgtgaaga
     1621 cgatgcgcgc ctcggtgtac gtgatggggc cgctcctcgc gcgcgcgggc gaggccgacg
     1681 tgtcgctgcc cggtggctgc gccatcggcg agcgccccat cgacatccac ctctccggct
     1741 tcgaggcgat gggcgcgacc atcgagctcg agcacggcta catcaaggcg cgcgccgagc
     1801 ggctctccgg cggccggttc gttatgcgaa atgtctccgt gggcgcgacc gccaatcttc
     1861 tcatgggcgc ggtcctggcc gacggcgaga ccgtgctcga gaactgcgcc aaggagccgg
     1921 acatcgtgga cctgggccgc ttcctggtga agatgggcgc gaccatcgag gggctgggca
     1981 ccgaccgcat catcgtccag ggcgtgccaa agctttcggc cgtcacctac cgcgtgatgc
     2041 ccgaccgcat cgaggcgggc acctatctgc tcgcgggcgc gatcacccgc gggtcggtga
     2101 agatcgtgcg cgccgagccc gaccatatcg gcgccctcat ccagacgctg ggcgagatgg
     2161 gcttcgcgat cgagaccggg agcgactggg tgtccatcgc acccggcgac cgggcgctct
     2221 ccggcgcctt catccgcacc ctgccctatc cggggttccc caccgacctg caggcgccca
     2281 tgatgtcgct catgaccacc gcgccgggca tcagcgtaat catcgaaacc atcttcgaga
     2341 accgcttcac ccacgtgggc gagctgcgcc gcatgggcgc ggacatcacc acggagggca
     2401 atgtcgccat catcaagggg caggagcgcc tctcctcggc caccatcatg atgtcggacc
     2461 tccgggcggg ggcagcgctc gtgctcgccg cgctcgccgc gcaggggacc acggagatcc
     2521 gccgcatcta ccattccgac cgcggctacg aaaaattaac ggagaagtta ttgggcctcg
     2581 gcgccgatat aaaaagggag gcaggaggaa aactgtaatg ctccgaggaa tctacaccgg
     2641 cgcgagcggc atgatcgcgc aacaggcgcg catggacgcg gtggcgaaca acctggccaa
     2701 tgtggaccag accggctata aaaaggacac cgccatattc aagacctttc ccgagatgct
     2761 gatccgccgc ctgagcgacg acgggctggg catcgtcccc acgggctcgt acgacaccat
     2821 gcccttcgtg ggcaagctgg gcaccggcgt cgaggtgaac gaggtgtaca cccagttcga
     2881 gcagggatcg ctccagcgca ccgagaactc cttcgacctc gcgctcgagg ggcgcggctt
     2941 cttcgccgtg caaaccgagc gcggcgagcg ctacacgcgg aacggcgcct tcaccatcaa
     3001 ccaggacggc gtcctcatga cgcacaacgg cgacccggtc ctaggcgaaa acggggtgat
     3061 ccgcgtccaa cagaacaact tcatcgtcaa cgagcgcggc gagatcatcg tgaaccaggc
     3121 gctgtcgctg gacccgcgcg atccggtgaa catgaccggc aacacctggg aatcgccgac
     3181 ggtgatagac cgcatcaagc tgatcgactt cgagcgcatc cgcgagctta aaaaggaagg
     3241 gaactcgttc tatcgagaga cgcccgagtc gggcccgccg ctcccgcccg cggagatcaa
     3301 ggtggtccag gggttcctcg agaaatcgaa cgtgagcgcc gtccgcgaga tggtggacat
     3361 gatcgaggtc cagcggagct acgaggccaa ccagaagacc atccagaccc acgaccagac
     3421 ggtcggcaag ctcatcaacg acatcgtccg gtaacgatca caccgtcacg ggccggccgc
     3481 acttcttctc gttgtcccag aagggggata tctcgagcag ccggtcgatg atgcccggca
     3541 gcacctcaag cgtgtaatcg atctcctcgt ccgtggtgaa ccggctgagg ctgaagcgga
     3601 tggagccgtg cgccgaggtg aacggcacgc ccatcgcgcg cagcacgtgc gaaggctcca
     3661 gcgatccgga cgaacaggcc gatccggacg acgccgcaat ccgcttctcg ttcaaataca
     3721 gcaggatcgc ctcgccctcg atgaactcga acccgacgtt cgtcgtgttg ggaacgcgcg
     3781 catcgcggct cccgttgagg ctcgcattgg gaaagcgttc cagtatctcg tgctcgagcc
     3841 ggtcgcgcat cgcggcgatc tcgtgctcgc ggttcaggta tatgcgcgcc agctccgccg
     3901 ccttcccgat ggccacgatg cccgcgacat tgtccgtccc ggggcgacgc ccgcgctcct
     3961 ggtgcccgcc gtacatgatg ggccgcatgc gcgtccctcg ccgcacgtac aggacgccca
     4021 cgcccttcgg ggcgtggaac ttgtgaccgg agagcgacag caggtcgcac tggattctct
     4081 tcacgttgat gggcaccttg cccgccgcct ggacggcgtc gatgtggaac gggacccccc
     4141 gctccctgag gaaggccccg atctcctcca ccgggaagat gacgccggtc tcgttgttgg
     4201 cgtgcatcac cgacacgatc gccgtatcgt cgtcgaccga cgccttgaga tgctccaggt
     4261 cgagctcgcc ggcgcggttc acgggcacat acgttacgcg gtatccgtcg cgctcgagct
     4321 tcttgcacaa attgagcacg gccgggtgct ccacccgggt ggtgatgatg tggcgctttt
     4381 cggtataata cgagatggcg ccgagaatgg ccatgttgtc gctctcggtc ccgcagctgg
     4441 tgaagacgat ctcgtagtcg ttctccgcgc cgatgagctc ggccacctgc gcccgggcgc
     4501 gctcgaggtg cgtcgccacg ccgccgccga aatcgtgcat gctggaggga ttgccgtagc
     4561 gctcgcgcag aaacggcatc atcgcctcga tcacctccgg atcgaccttc gtggttgcgt
     4621 tgttgtcgag gtacacccat tcctcgcgcg attgccgctg gtgcccgtgc cgcatatcag
     4681 ttccgctcct tcgtcatcag aataccccgc gtcggtatca tttcgcctcg accaccacga
     4741 tatcgtcctc gacgaactcc cgcagcttgt cctccacgag gttcttgagc gtcacgtcgg
     4801 agagccggca gctcgcgcac gcgccgcgaa gcgccacgat cacccgcttc ccctcgatgt
     4861 cgacgaggtc gatgttcccc ccgtcgcgct ggagaatggg ccggatgatg ttgttgatgg
     4921 tctcctccac cagctggatg cgcctgatat tcgtgagcgc gcgggggtgc tcctcgggtg
     4981 cgggttcggc ctggtgctgg cgcagctcct cgcggatgat gtgctcgatc tccgggatgc
     5041 acgatccgca agcgccgccc gccttggtgt agttggtcac gtcctcgacc gtcttgagat
     5101 tgttctcgcg gatcaccttg cggatgagcg tatcggtgat gccgaaacac tggcagacaa
     5161 tcgcgccgtc cgtgtccgcg tgctcctcga tgctcacccc gcgccagttc gcgagcgcct
     5221 tgtcgagcgc ctcgcgcccc atcaccgagc agtgcatctt ctgctcgggc agcccgccca
     5281 ggaagtccac gatgtccttg ttggtgatgg tctccgcctc ggcgacggtc tttcccttga
     5341 tcatctccgt aagcgccgac gaggacgcga tcgcgctccc gcacccgaag gtctggaatt
     5401 tcgcgtcgac gatgacatcg ttctcgatct tgagatagag ggtgagcgcg tcgccgcaga
     5461 cgatgctgcc cacctcgccc acggcgttgg cgtcatcgat cctcccgacg ttccgcgggt
     5521 tggtgtataa atccttaacc gtatcggtat agtcccacat gcattcaact ccatcaacgc
     5581 cctacgggcg tactgcctcc acaacagcaa ccgaccatgc gtcattcact caatacaata
     5641 ccacaaacct aagatagccg ctcatttcag tatttcataa aaatggcgca tccggatgag
     5701 cactgtcaaa cagaaatggg gcacggaaaa aaaaatcccc gcacatgcag ggataagcgt
     5761 ccaagaggtc tcctgatgag agtttggcaa ctcccaccaa aggattggtt atgaatgcga
     5821 aattgaagaa aaacctacga ccatacgaac tgaattataa tataatcgcc gatttacaat
     5881 aatgcaagta aaaaaatgaa cacatcttta attaatttca attccaaccg gacggttttt
     5941 caatttttgc ccgccctcca gctatccggg aaagcgcaat acgcaaattt tcaattcagt
     6001 taaaccgctt ttctaacgat agatatgata tgaaaatcta accgtgcgag attatagaca
     6061 ctatggttaa attagtccac aatgatctgc ttggcatgat cagggacagc aaaacagccc
     6121 cggatgccgg cttacaggac atgccgggcg catcggtccg tttcgacgac atcctcgcgc
     6181 gacagctgca cgagacgaac aagccgtccg aaccggaatc acggcgcgaa tcagcactgc
     6241 aaccgcgcga ggacgctcgc cctcccgatt cgcccgccga aaccgcccat cacccggccc
     6301 cccgcgcgag cgaagacgac agccgcgtgc gcgacagcga ggccaatcac gaaccggaga
     6361 caaaagaaca gaacgccatc agcgaaaaag cggacagcgc acagcgcaat ggtgatgata
     6421 gcgacaccgg ggaacaatcc gacgaggacc accggacaca ccgcggccgc acgatcgatc
     6481 ccctgctgat ggacctggtc aatccgcgcg ccaccgacgc caagcccggc ggcaacgccg
     6541 cgatccagga cctggtcgaa aaaacccgtg cggcactcga tgcgatcatc aagaagggcg
     6601 cgtcgaaaac cggcgacgcg cgtgatgccg cactcatgaa caccctgcgc gaattgggcc
     6661 gcgagctcga ggccctttcg gggaacgcct ctccgcaggg acaccggcag cagctcctgg
     6721 cgctgttgca gaaaatcaag acgaacatct ccacgctcga cggcaacgcg gcgcatgtcg
     6781 ccgaccggga gctcggacaa ttgatccaag gactcaagaa acacatcggc gagatcatga
     6841 ccgcgctttc aaaaagcgac aagggcgccg cgcaggattt cgcatcgacg atgaaccagc
     6901 gtcgcgagtc cggcgagccg cgcccggcga ccgagagggc gttcgttccg caaaccgcgg
     6961 catcgatcga cgccgtcgaa caccgcccgt ccggaaacaa cgacagcgcc ctcggctttc
     7021 agttctcgaa gcacggcgcg cagacgatgc ggatgggcga gcccggccag cccgcgccgc
     7081 gcgcgggact cttcggcgag cagctccagc aggtgatgga gaacgcgaag gtgttcattc
     7141 gcgacagccg gaacggatcc ttctcgctgc agatgcaccc cgagtcgctg ggcaaggtga
     7201 acgtgagcct gggcctggac cacggcgttc tctccgggcg cttcttggtc gaatcgaacg
     7261 aggcgcgcga tctgctgctc gagaacatcg accagctcaa ggagaagctc gccgaggcgg
     7321 gcgtctccgt cggagaattc aacgtaaacg tccgcgatca ccgcgagcat cggttcgacg
     7381 agcgcgagca ggagacggaa ttcatccatc gcggcatgcc ccgcgaggcc ggcgccggtt
     7441 acgacgcccc cgctcgctcc cggcacgacg gcgcgatcga catggtaata taggagcgac
     7501 acatggacaa gctcactaaa atggattcac gggatctcat gcgcacgcag cagcgcgtgg
     7561 acgagatcaa caagaagttc cgcaaacccg acggcaagct gaagggggag ctgggaaagg
     7621 actcgttcct gaagctcctc gttacggagc tcaggcacca ggacccgacc aacccgatgg
     7681 ccgacaagga gttcatctcg cagatggcgc agttctcgtc gctcgagcag atgcacaatc
     7741 tgaacaactc gatgaaggga ctcacggaca tggcgcaatc gaacaaggcc tactccctgc
     7801 tcggaaagag cgtcgaggcg gtggacccga agaccgggaa agtggtccgc ggggccgtta
     7861 cgggaatcac ccacaacgga aacgacattc gcatcaagat aggcgccacc gaggtggggc
     7921 tggccgagat acacggcgtg tacgagaacg gcccggccga gaagaagaac gcggcgccgg
     7981 atgcgccccc ggcgatcaag cggaacgaac cggccggcgc gcaccagatc aaggcgtatc
     8041 agctttttta accatgcaat ctcacgcgaa caacgatatc cgcgcacaag gagttaaccc
     8101 actatgatgc gatcactgtt ttccggtgtc tccggcctga agaaccatca gactcggatg
     8161 gacgtcatcg gtcacaacat ctcgaacgtg aatacctacg ggtacaagta cacccgcgcg
     8221 accttccagg act
//
LOCUS       DAORHF010000168         8228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8247_length_8228_cov_5.090787, whole genome shotgun sequence.
ACCESSION   DAORHF010000168 DAORHF010000000
VERSION     DAORHF010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8247_length_8228_cov_5.090787"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="gltA"
                     /locus_tag="PKJ16_13040"
     CDS             complement(<1..1296)
                     /gene="gltA"
                     /locus_tag="PKJ16_13040"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HNV47962.1"
                     /translation="MDKPKELTAQERRKIPRQEMPMQPPAESGRNFSEVATGFTEASA
                     VAEADRCIQCKNPKCVKGCPVEVPIPEFIKALREGQMSESARILKSTNNLPAVCGRVC
                     PQETQCELECILGIKGEPVAIGRLERYVADWQLANEKPRVERAKPTGKRVAIIGTGPA
                     GLTCAADLAKMGHAVTMFEALHVAGGVLMYGIPEFRLPKAIVQAEIDYVRELGVEILL
                     DQVIGKIFTLDELLNEEKFDAIFLGTGAGLPTFLNIPGENNNGVFSANEFLTRVNLMK
                     GYLFPEWDTPVSVGRKVVVFGAGNVAMDAARCALRIGNIMSQKNGTPPPKVHIVYRRS
                     REEMPARAEEIHHAEEEGVELNLLTTPLEILADEKNNVRAVRCLQNRLGDPDASGRRS
                     PVPIPGSEFEIEADTVIVAIGTSPNPLVFHDAAGLERGKW"
     gene            complement(1352..2194)
                     /locus_tag="PKJ16_13045"
     CDS             complement(1352..2194)
                     /locus_tag="PKJ16_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HNV47963.1"
                     /translation="MYEILRKTVLSNAVKEIDVAAPQIAKKGKAGQFVVLRVHEQGER
                     IPLTIADFDREKGFVTLVYQEVGKSTRHLGTLGVGDSLASLTGPLGHASDIKKYGTVV
                     CVGGGVGIAPLYPIARELKRAGNRVLSIIGARNRDLLFWEDKMAAISDRLIVCTDDGS
                     HGRKALVTEPLKELFESGEETIAHTWAIGPAIMMKFCCLVTKSFNAPTTVSLNTIMVD
                     GTGMCGACRVKTATKTYFACVDGPELDGHEIDWDLLLSRQGQYLNLEKQADERYRSSC
                     ACGS"
     gene            2583..3635
                     /locus_tag="PKJ16_13050"
     CDS             2583..3635
                     /locus_tag="PKJ16_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47964.1"
                     /translation="MIDTGPRSAHGAPERADTHCIRCGNACAITLETRDGDLVRIRPT
                     NGEPDALADACCAQCPACCPGHDRPEVAVDQKLPFQMMYFTSGYDRNRILVSLSPTLT
                     VEEIYMVAESARAHSHALYSFTLAADLEKIDLLAAHGIPASGASASHDSYETVYYFGA
                     CRGIAAAATLRRLVDASPQRALMCADAIPAPYLAGASLGSDEALLAHLIAHDTPSTLV
                     AFTLQDISTAALAPLADYIAARQAGGLAILHASVNYHYLRNLIPDLRATRDRIAEGHY
                     HLLTCVNESDRLVSGPFEEMIVFTDTVSHLNAKYIIPIDAPCEKAGTVIDQFGAYKSV
                     RGPLRRRGYPLELFFS"
     gene            3743..4672
                     /locus_tag="PKJ16_13055"
     CDS             3743..4672
                     /locus_tag="PKJ16_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV47965.1"
                     /translation="MFSRMTTIALAVTLAGTVFFCKHAPKITKAFVLNFIGEVQLVNA
                     QKEARKPEIKAGVDESDTVQTGEKSEITIQLRDTGVVRVFEKTEVLFKAIAQNGTNTD
                     ISLANGAIFSKIKKLTADKKYRITTPTLTIAIRGTEFLTEYSDEKKETTISVKEGEIV
                     ITLLKAAVAAPQEIPVKQGETVTIDKDQKVRRGPTMSIRAAELEKYSLHHYLDNLDAM
                     TIEQIKEHFRKLKLAEDEIDKKIEFLKELAKLSPLDRLRKLGKRITMFHMKDGSKIAG
                     NVVSQDALNITIDTGDGIITIGKNDFRRREDIK"
     gene            4760..5623
                     /locus_tag="PKJ16_13060"
     CDS             4760..5623
                     /locus_tag="PKJ16_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47966.1"
                     /translation="MKKILTTCRWIALEIPLIIALMTPAAAEVVYLKDGQVIKGAIIQ
                     EDDQSVTVQTKHKTWNIKREDIQRILFGEEEMELMYLLMNDGKVVKGYIVDQDNTKVI
                     IRRDKDKAQEETILKKDIKQMSPEEIVLLEPEVAVLGGIYHPLNPGTSELKPSPYALL
                     VTDFNLPYVRNMRIRIAAGFSDCTSAKYDQIKFKVIPVLFGAEYVFKLSFFSIVPHLS
                     LGTAILDYDNGEGKTYRGYKGLGVAGLGFNFELIKNFLFVKTLVEYGIIIDEGTTMSN
                     LFYTAGLGIRF"
     gene            6457..>8228
                     /locus_tag="PKJ16_13065"
     CDS             6457..>8228
                     /locus_tag="PKJ16_13065"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV47967.1"
                     /translation="MGQEGSGSFTIINDDDEPIATATPGSLTSADPVSVTLDTHETLS
                     SDLSYVVSTTPNPADPNFTGNGTVVAGPLASIVIGSAGDNQTYYVKYVARDSVGNVGP
                     IGSATYRIFAAAPSFYNVTTSRDFVSTAGYTTSTLSWTCNMDGDYTITANGSVRLSGR
                     ATADVPVDSVIQASWLASGNNTITITVTGDNLQVGQTTDVIHRDDTAAGTNVITAPTA
                     GNRVRQSTTISANAIDSESGIEKVEFFVDGPLHATDTATPYSYNWDTTLNSNGNHNLT
                     IRTTNGAGVAATTAATVVYVDNAVPSVNLTAPASGARVNNATTISATANDNDSIKRVE
                     FHLYPEGTMIDLDTTAPYSISWNANAATEGNHTLRATAYDQADNTAISEIPIYVDKTN
                     PIANVETPDNGNFLKGNNVFEVSASDPNGGTIARVDLLIDGALCGQKTAAPFLFDVNT
                     ANYANTTHNISARAFDSAGNQVLDDDTTVTFDNVNPSVNITSHINGDNVRGNAVSVAI
                     SASDNTSVSKVELYIGANPTPVATFTAEPYTYVWNTTSGSYPDGSYALRAIAYDAAGN
                     IDTDNDTTVIVDNTNPTVSITVPAP"
BASE COUNT         1816 a         2637 c         2227 g         1548 t
ORIGIN      
        1 ccacttgccg cgctcaagac ccgcggcgtc gtgaaagacg aggggattgg ggctggtgcc
       61 gatggcgacg atcacggtgt cggcctcgat ctcgaactcg gagcccggga tcggcacggg
      121 gctgcggcgg ccgctcgcgt ccggatcgcc gagtcggttc tgcaggcacc gcaccgcgcg
      181 cacattgttt ttctcgtcgg ccagtatctc gaggggcgtg gtgagcaggt tgagctcgac
      241 gccctcctcc tcggcatgat ggatctcctc ggcccgcgcc ggcatctcct cgcgcgaacg
      301 gcgatagacg atatgcacct tcggcggcgg cgtgccgttc ttctggctca tgatgtttcc
      361 gatgcgaagc gcgcagcgcg ccgcgtccat cgccacgttc cccgcgccaa agacgaccac
      421 cttgcggccc acgctcaccg gcgtgtccca ctcggggaag aggtagccct tcatgagatt
      481 cacccgggtg aggaactcgt tggcggagaa cacgccattg ttgttttcgc cggggatatt
      541 gagaaacgtg gggagccccg caccggtgcc caggaagatc gcgtcgaact tttcctcgtt
      601 caggagctcg tcgagcgtga agatctttcc gatgacctgg tccaggagga tctccacccc
      661 cagctcccgc acataatcga tctccgcctg cacgatcgcc ttcggaaggc ggaattcggg
      721 aatgccgtac atgagcaccc cgccggccac gtggagcgcc tcgaacatgg tgaccgcgtg
      781 ccccatcttg gccaggtcgg cggcgcaggt gagccccgcc ggccccgtgc cgatgatcgc
      841 gacccgcttg ccggtcggct tcgcccgctc gacgcggggc ttctcgttgg ccagctgcca
      901 atcggccaca tagcgctcca gcctgccgat ggcgaccggt tctcccttaa tccccaggat
      961 gcattccagc tcgcactgcg tctcctgggg acatacgcgg ccgcacacgg cgggaaggtt
     1021 gttggtgctt ttcaggatcc gcgccgattc gctcatctgt ccctcgcgca gcgccttgat
     1081 gaactcgggg atgggcacct cgacgggaca gcccttgacg cacttggggt ttttgcattg
     1141 gatgcagcgg tccgcctcgg cgacggcgct cgcctcagtg aagccggtcg cgacctcgct
     1201 gaaattccgc cccgactcgg ccgggggttg catcggcatc tcctgccggg ggatttttct
     1261 gcgctcctgc gctgtcagct cttttggttt atccatggcc ctttatctcg atcccaatgc
     1321 aaagatagta tcggcgccga acggcgcatg gtcacgatcc gcaggcgcag ctcgaacgat
     1381 agcgctcgtc cgcctgcttt tcaagattta aatactggcc ctgccgcgac aggagcaggt
     1441 cccaatcgat ttcgtggccg tccagctccg ggccgtccac gcacgcgaaa taggtcttgg
     1501 tcgccgtttt cacccggcag gcgccgcaca tgcccgttcc gtccaccatg atggtgttca
     1561 ggctcaccgt cgtcggggca ttgaacgact tggtgaccag gcagcagaat ttcatcatga
     1621 tggcggggcc gatggcccac gtatgcgcga tcgtttcctc gcccgattcg aagagctcct
     1681 tgagcggttc ggtcaccagc gccttacgac cgtggcttcc gtcgtcggtg cacacgatga
     1741 ggcggtcgga gatcgcggcc atcttgtcct cccagaagag gaggtcccgg ttgcgcgcgc
     1801 cgatgatgga gagcacccgg ttccccgcgc gcttcagttc gcgcgcgatc ggatagagcg
     1861 gggcgatccc cacgccgccg ccgacgcaca cgaccgtccc gtatttcttg atgtcggagg
     1921 cgtggcccag cggtccggtg aggctggcca gcgaatcacc cacccccagg gttcccaggt
     1981 ggcgggtcga tttccccacc tcctgataga ccaaggtgac gaagcccttc tcccgatcga
     2041 aatcggcgat ggtcaggggg atgcgctcgc cctgctcgtg cacgcgcagc acgacgaact
     2101 gcccggcctt acccttcttc gcgatctgcg gcgccgcgac atcgatttcc ttgacggcgt
     2161 ttgacagcac ggttttacgg agaatttcgt acatagcacc aaccatagcg gagcggcgtt
     2221 ccagcctacg aaaagacgcc ctcactgcga aacgtcaatc atcccggttt cgcagaatcg
     2281 gaaaaacgga acgcccttcg tgagcaatta gcgacactaa aaaaaattat ggccaaatta
     2341 gtatagaaaa agtaaagatt tttcctttac acgatggaat ttttttcatg cacatatctg
     2401 taagccgatc tgcaccgcac ggtatgcgcg caccgtccgt ccgcacaacc tacacggatg
     2461 tccagtgggg aaatgagcgc cagtcgttag actgcgttct tatctattga caaaactcaa
     2521 cggcaatggg atgattcggc caatgcgcca caggcaccac ataccagtac ggcggccgtg
     2581 tgatgattga caccggcccg aggtccgccc acggcgcgcc cgaacgcgcc gacacccact
     2641 gcatccgctg cgggaatgcc tgcgccatca cgctcgaaac gcgcgatggc gacctcgtcc
     2701 gcatccgccc gacgaacggc gagcccgatg cgctggccga cgcctgctgc gcgcagtgcc
     2761 ccgcctgctg cccgggccac gaccgtccgg aggtagccgt cgatcaaaaa cttcccttcc
     2821 agatgatgta tttcaccagc ggctacgacc gcaaccgcat cctggtgtcc ctttcgccca
     2881 cgctcaccgt cgaggagatc tacatggtgg cggaatcggc gcgcgcgcac agccacgcgc
     2941 tgtactcctt cacgctcgcc gccgatctcg aaaaaatcga tcttctggcc gcgcacggca
     3001 tccccgcaag cggcgcatcc gccagccatg attcgtacga gacggtgtat tatttcggcg
     3061 cctgccgcgg gatcgccgcc gccgcgacgc tccggcggct cgttgacgca tcgccgcaac
     3121 gagccctcat gtgcgccgac gcgattccgg cgccatacct cgctggtgcg agcctgggat
     3181 cggacgaggc gctgctcgcc cacctcatcg cgcacgacac cccctcgacg ctcgtcgcct
     3241 tcacgctgca ggacatctcg acggcggcgc tcgcgccgct tgcggactat atcgccgcgc
     3301 gccaggcggg cgggctcgcc attctgcacg cctcggtgaa ctaccactat ctgcgcaatc
     3361 tcatccccga cctgcgcgcc acgcgcgacc gcatcgcgga ggggcactat catcttctca
     3421 cctgcgtgaa cgaaagcgac cggctcgttt cgggaccgtt cgaggagatg atcgttttca
     3481 ccgacaccgt ctcacacctc aatgcgaaat acatcatccc catcgacgcg ccctgcgaaa
     3541 aagccggcac cgtcatcgat cagttcggcg cctataaatc ggtgcgcggc ccccttcgca
     3601 gacggggata tccgctcgag ctgttctttt cgtaaacggg gcgaatgcgt gatccgtgac
     3661 cgcataaata aaaattattg acattttata aaattgctcc tattttagta tcactaatta
     3721 atcacgatca atggagtacg acatgttttc acgcatgacg actattgcgc tcgcagtcac
     3781 actcgctggc accgttttct tctgcaagca cgcaccgaag atcaccaagg cgttcgtcct
     3841 caacttcatc ggcgaggtgc agctcgtcaa cgcgcagaaa gaggcgcgga agcccgagat
     3901 caaggcgggc gtcgatgaga gtgacacggt tcagacgggc gagaagtccg agatcaccat
     3961 acagttgcgc gatacgggcg tggtgcgcgt ctttgagaag acggaggtcc tcttcaaggc
     4021 gatcgcccag aacggcacca acaccgatat ctcgctcgca aacggcgcca tcttctcgaa
     4081 gatcaagaag ctcaccgccg acaagaaata ccggattacc acgccaacgc tcacgatcgc
     4141 gatccgcggg accgagttcc tcaccgagta cagcgacgag aagaaggaga ccacgatatc
     4201 cgtcaaggag ggcgagatcg tcatcaccct gctcaaggcg gcggtcgccg cgccgcagga
     4261 gatcccggtg aagcagggcg agaccgtcac cattgacaaa gaccagaagg tccgccgcgg
     4321 acccaccatg tccatccgcg cggccgaact cgaaaaatac tcgttgcacc actatctcga
     4381 caacctggac gcgatgacca tcgagcaaat aaaggagcat ttccggaagc tcaagctcgc
     4441 cgaggacgag atagacaaga agatcgagtt tctgaaagaa ttggccaagc tctcaccgct
     4501 tgaccggctt cgcaagctgg gcaagcgcat caccatgttc cacatgaagg acggctcgaa
     4561 gatcgcgggg aacgtcgtct cgcaggatgc gctgaatata accatcgaca ccggcgatgg
     4621 catcatcacc atcgggaaga acgacttcag gcggcgcgaa gacatcaaat agcgcctgcc
     4681 cccacccgac ctccctcttc gatcggggga ggtatcaaag aaagacgaac gtctaacgta
     4741 gttttgcgga atagtggaaa tgaaaaagat acttactaca tgccgttgga tcgccctcga
     4801 aatccctctc atcatcgcgc tgatgacacc cgccgccgcc gaggttgtct acctcaaaga
     4861 cgggcaggtg atcaagggcg ccatcatcca ggaggacgac cagtcggtca ccgtccagac
     4921 caagcacaag acctggaaca tcaagcgcga ggacatccag cgcatcctct tcggcgagga
     4981 ggagatggag ctgatgtacc tcctgatgaa cgacgggaag gtcgtcaagg gctacatcgt
     5041 ggaccaggac aataccaagg tcatcatccg ccgcgacaag gacaaggcgc aggaggagac
     5101 catcctcaag aaggatatca aacagatgtc gcccgaggag atcgtcctct tggaacccga
     5161 ggtggccgtg ctgggcggca tctatcatcc cctcaatccc gggacttccg agctaaagcc
     5221 ctcgccctat gccctgctgg tgacggattt caacctgccc tatgtccgca atatgcgcat
     5281 ccggatcgcg gctggctttt ccgactgcac aagcgcgaag tacgaccaga taaaattcaa
     5341 ggtcatcccg gtcctctttg gcgccgagta cgtcttcaaa ctgtcatttt tcagcatcgt
     5401 accccacctg agcctgggga cggccatcct ggactacgac aacggcgagg ggaagaccta
     5461 ccgtggctac aagggactcg gtgtggccgg gctgggattc aacttcgagc tcattaaaaa
     5521 cttcctcttt gtcaagaccc tcgtggaata cggcatcatc atcgacgagg ggaccaccat
     5581 gagcaatctc ttctacaccg ctggccttgg catccgtttt taatattcat ctttgaatat
     5641 gctcacaaat tataaaaagt acaaaaatgt ccttgattca tttgactgag ttcaacattt
     5701 ggtgtaaatt aatacggata tatggacttt ttattaactt gaagtctata aaaataaaca
     5761 aagagcactg atatgatcag ggaatactta gcggtactcc tcatttcatt ttttctccta
     5821 acctcgcttg cctgtggaga caggatgcta gaggagatac agcagtttaa gccaaatcaa
     5881 ataccagaaa tcaaggcctt cacgtccgac gtggccaccg gaaccgccat ggaacccaat
     5941 atggtcatcc atttatccgc agaggcaacc gatcccgagg gtgaggagct ccgatatgaa
     6001 tataagtccg attacggttt ctttcaaaac caattggata ccggcaatac cagcacagtt
     6061 accttcataa ccaacgacca agtccgaccg aacatccctg tcaccgtaac gcttaccgta
     6121 tcagatccaa aagagtcctc tgtggtacag attcttgaaa ttggagaagg taactccggg
     6181 ccaagtgtta gcacctccgc tccagcggac cgtatcaaat cagattccac cgtcggcgcg
     6241 agcttcacta gcagcgggag cggattctac caggttcatg ttgtcgatga tcccaacgga
     6301 accgcgacat ttgacaccgg caaaccggca acatactgct cagcgggaag taccgttaat
     6361 ttcacactct atggttccga caccgcgagc cacctctctc cacgactgtt tcccggcacc
     6421 gcaggagacc gaattgtcat agtagtactg gatgccatgg gtcaggaagg cagcggttcg
     6481 tttaccataa taaacgacga cgacgagccg atagccacag ccacgccggg aagcctcacc
     6541 tccgccgatc ccgtctcggt gacgcttgac acgcacgaga cgctttcgtc cgacctgtcg
     6601 tatgtggtca gcaccacccc caatccggcc gatccgaact tcaccggcaa cggcacggtg
     6661 gtcgccggtc cccttgcgtc gatagttatc ggcagcgccg gcgacaacca gacctattac
     6721 gtgaaatacg tcgcgcgcga ctcggttggc aatgtcggcc ctatagggag cgccacctac
     6781 cgcatttttg cggcggcccc aagtttttac aatgtgacca cctcgcgcga tttcgtgagc
     6841 accgcgggct ataccacgag cacgctctcg tggacctgca acatggacgg cgactacacc
     6901 atcaccgcca atggatcggt gcgattgagc ggccgggcga ccgccgacgt cccggtggac
     6961 tcggtcatcc aggcctcatg gctcgccagc ggcaacaaca ccatcaccat aacggttacc
     7021 ggcgacaatc tgcaggtggg gcagactacc gacgtcattc accgcgacga tacggcggcc
     7081 ggcaccaacg taattaccgc ccccacggca ggcaaccgcg tacgccagtc cacgaccatc
     7141 agcgcaaacg ccatcgactc ggaatccggc atcgagaagg tcgagttctt cgtcgacggc
     7201 cctcttcatg ccacggacac cgcaacgcca tacagctaca actgggacac gacgctcaac
     7261 tcgaacggga accataacct caccatacga accaccaatg gcgcgggcgt cgcggctacc
     7321 acggcggcca ccgtggtgta tgtggacaac gccgtcccgt cggtaaacct aaccgcgccc
     7381 gccagcggcg cgcgcgtgaa caatgcgacc accatatcgg caacggcgaa cgacaatgac
     7441 tccatcaagc gggtggaatt ccacctttat cccgaaggca cgatgatcga tctggacacc
     7501 actgctccat acagcatctc atggaacgcg aacgcggcaa ctgaaggcaa ccacaccctt
     7561 cgcgccaccg cctatgacca ggcggacaat accgcgattt cagaaatacc catctacgtg
     7621 gacaagacca atcccattgc caatgtggag acgcccgaca acggcaattt tttgaaaggc
     7681 aacaacgtct tcgaggtttc cgcgtccgat cccaacggcg gcaccatcgc ccgggtggac
     7741 ctgttgatcg acggcgcgct gtgcggccag aagacggcag cgccctttct ctttgatgtc
     7801 aataccgcca attacgcgaa cacaacgcat aacatctccg cgcgcgcctt cgacagcgcc
     7861 ggaaaccagg tgctggacga tgacaccact gtcacgttcg acaacgtgaa tccgtcggtg
     7921 aatatcacca gccacatcaa cggcgacaat gtgcgtggca acgccgtaag cgtggccata
     7981 agcgcaagcg acaacaccag cgtctcaaag gtggaactgt acatcggggc caacccgacg
     8041 cccgtcgcga ccttcaccgc ggagccatac acctacgtat ggaataccac ctccggttca
     8101 tatccggacg gttcgtacgc gctcagggcg attgcctacg acgccgccgg caacattgac
     8161 accgacaatg acacgacggt tatagtggac aacaccaacc cgacggtgag catcaccgtc
     8221 ccggcccc
//
LOCUS       DAORHF010000169         8169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8340_length_8169_cov_4.802687, whole genome shotgun sequence.
ACCESSION   DAORHF010000169 DAORHF010000000
VERSION     DAORHF010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8169
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8340_length_8169_cov_4.802687"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2325)
                     /locus_tag="PKJ16_13070"
     CDS             complement(<1..2325)
                     /locus_tag="PKJ16_13070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV47968.1"
                     /translation="MNWLRSHIVLVVAIGVALAYLSGAVLSYEFFIKYLLAASGLSWR
                     GDFLILVHLAISCVFIGLGAFLDRQYIRNRAVAQAARLHSLAVRYSSDGIAVIGRDGV
                     LRYASPGIERIFGYTAADFGNLAEIIDRVVPDPEVRQTVVANWLYDRDADNSPDRIYP
                     IVTGSGDARWCRIRCTRMNADELVVNALDVTDLVRAEEAIKASEEKYRDLFTNAQVGL
                     YRTRIDDGCVIECNDRFARLLGYERRESCIGTCVVLDHYVNPDRRDDMLRELRSSGTV
                     RDFESEYRAKDGALRWLSVTARLFGDAGYIEGAAIDITERRRVERAFQLSEERYRLVV
                     ENANEGIIVVQDGFIRFSNPKACEITGYTEREISAIPVMDLIHPDDRTPVGDRYLKYT
                     RRNKPAHQYEFRIIEKGGAIKWLFVNVVAVDWEQGRAALLFFSDINRRKKAENAVREE
                     RDRAQRYLDIAGVIIVAIDGSRRVTLINKRGCDILGYAEDEIVGREWFADFIHPEDAG
                     WLAEQYEAMMTQGRLYSDWYQVARIVTREGQDRIIAWHNTVLTDDFGVPSGILSSGED
                     ISEQRRAEAERAKMQAQLLHSQKLEAIGTLAGGIAHDFNNLLTAIKGSVDLALFEVDE
                     GHPIHPNLKQIQHAAVRAADLTRQMLVFSRRQMMESAPLNINRTIENLLKMLKRLIGE
                     DVAITTHLAHDLRDVYLDEGAIEQVVVNLSVNARDAMPNGGQLIIATHNRFFDADESS
                     DMPEARPGEFVCLTITDTGIGMAPEVVGRIFEPFF"
     gene            complement(2461..6774)
                     /locus_tag="PKJ16_13075"
     CDS             complement(2461..6774)
                     /locus_tag="PKJ16_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV47969.1"
                     /translation="MHSLGINIGSSSVKVAMLEGNGILWSEVVAHEGNIFDTLRKVMQ
                     GKDFPADLKTLVTGIEGRHLLTVNSVIEPLCVEEALARVNTKVDAVVSMGGEDLVVYT
                     IDANGKIVNSFSGNKCASGTGEFFKQQLGRMDMRLDDINGIPEGCTVHKLSSRCSVFM
                     KSDCTHRLNKKEASKGDIVLSLSDVMATKVVDFLKRARIGGGAVLLAGGVTRNKYIVD
                     YIRRRLPELTFVVPDNAAYFEAYGAGLLARSIGTVLPPLKKLFRENRVVFERFKSLKT
                     AEGKVTYIPSKMTKVRPNREYVLGVDGGSTTTKACLIDIETNEITANFYGRTHGDPVQ
                     ALKECLVEMKKQIKEDIGDAPISISLAATTGSSREILGVFLETPGVYNEIIAHSVGTT
                     FYANDIDTLFEIGGQDAKYVLLKNGVPIDYAMNEACSAGTGSFLEESAQGDLSIEHAW
                     EIGDIAIMADEPLKFGEHCSAFINSDIRKAIQQGATREDITAGLVTSIVSNYLNRVVG
                     NRTIGNKIVLQGGVAKNKAIPLAFALLLNKPIIVPPDPELMGCFGVGLLAKQKLADGF
                     LEKTTYDIDEILATEIVYEREFKCQACENYCPIRVLKVNEHRYMFGGRCNKYANIRKK
                     KAFDEAEVFNYIDARNDVLFVECAPDPATFVKKRDVTVGVPRCFSVYTMWPFYSWFFH
                     SLGIEAVLSKNISHEGVARVESAYCFPAEIAHGAVQDIVDRGTDYIFVPHFRDLEGYE
                     EGLIANFCPITQSLPYYIAKAFPDVPESKFLKPVITFHYGLDKAIESFLELGDNLGVS
                     HGDVRKAFETAYEKQIEFIHKIRDLGKRAIEEARIAKRPVIAVLGRPYNAFTPDANMG
                     IPKKFTTRGYSVVPFDILPFENELIFKNMYWFYGQQDMKAANFLKKEDNIFVTYISNF
                     SCAPDSFMLHYLKWIMGVKPFLILELDSHTADAGVDTRVEAFLDIIEGYRSKLGDIAA
                     ERYDNGLRFINERGKDFYIKNAVTNEIIPIKDNPRVKLLLANMGRLGVELLAASVRSA
                     GINASAMPVPDIHTLQLARNHASGKECLPSLLVLGSALKFLASDKYRKDEVYLLFVPT
                     TTGPCRTGQYFVFYENLFRDLRIENVLMITLDSDNSYTELGPNFSKFAWWGVIISDYM
                     KDIETSLRSCAENPVAAMDRYDALWHKMIGVAEGDVTKIVPHLQEFAREIRKIPLKRK
                     QEECPRVLIVGEIYVRRDDFAVDEMVRRFSEKGIIGKVSGVSEWIYYCDYTRHFELKK
                     RLSLIPFYRRYFSKDFRDLINWHVEQWYKHRVEHTVKKALAATGLIPETPHDMKKIMK
                     NANTIFVTPELYSEISISSGVASAAMQDGYSGVVNISPFACLIGRVIEGLITPWARER
                     KYPVMSIEIDGNLLPPNVINRMEIFMLNVQRFKENPGAAAFVEYHDSDRVSVDRKIIR
                     E"
     gene            7068..>8169
                     /locus_tag="PKJ16_13080"
     CDS             7068..>8169
                     /locus_tag="PKJ16_13080"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV47970.1"
                     /translation="MEHIIQILSEIPIFQGLSREDLALIVPILSTHELPAESLVVKEG
                     DIGDSMFIIKSGTVRVTKTEENGSEVLISHLYKGQYFGEFSLIDNLPRSANVVTEEAT
                     EIFTLNRGDFDRLLAANIVIKAAFHQNCLKETFSRFRNIISNFTFAQHDLREKREALD
                     EINRDLSLAKKLQRYFINTDYLRTEHTADNGVRHSYIYKPSIAIGGDFLNVFQLRDGV
                     VGMIIADFEGHGITAALATGVLKSALSIVLADLGEKPIELMSFLNDHFHKVIAQLYAT
                     CYYAVIHTEERRIVLAKAGHHHPLVWKARLNDFTPIECPGTGLGLMPNARFGVVEQDI
                     EPGDKVLFFTDGIIEQMNGFHEMYSKKRMTEKV"
BASE COUNT         1535 a         2574 c         2496 g         1564 t
ORIGIN      
        1 gaagaacggc tcgaagatcc gaccaacgac ctccggcgcc atcccgatcc cggtgtcggt
       61 gatggtaagg cacacgaact cgcccggtcg cgcctccggc atgtcgctgg attcatcggc
      121 atcgaaaaac cggttgtgcg tggcaatgat caactgcccg ccattgggca tcgcatcacg
      181 ggcgttcacg ctcagattca ccacgacctg ttcgatcgcc ccctcgtcga ggtacacgtc
      241 ccgaaggtcg tgtgcgaggt gggtggtgat ggcgacgtcc tcgccgatga ggcgtttaag
      301 catcttcaag aggttttcga tggtgcggtt gatgttcagg ggcgccgatt ccatcatctg
      361 ccgccggctg aagacgagca tctggcgggt gaggtccgcc gcgcgcacgg ccgcgtgctg
      421 gatctgcttc aggttcggat ggatcggatg gccctcgtcg acctcgaaga gcgccaggtc
      481 caccgagccc ttgatggcgg tgagcagatt gttgaagtcg tgcgcgatgc cgccggccag
      541 cgtcccgatc gcctcgagtt tctgcgagtg taaaagctgg gcctgcatct tggcgcgctc
      601 cgcctcggcg cgccgctgct ctgagatgtc ctcgcccgag ctcaggatgc cgctggggac
      661 gccgaagtca tcggtgagca cggtgttgtg ccaggcgatg atccgatcct ggccctcgcg
      721 cgtcacgatg cgcgcgacct ggtaccaatc gctgtacagg cgcccctgcg tcatcatggc
      781 ctcatactgc tcggcaagcc agccggcgtc ctccggatgg atgaaatcgg cgaaccactc
      841 ccggcccaca atctcgtcct cggcgtatcc cagaatatcg cagccgcgct tgttgatgag
      901 ggtgacgcgc cgggagccgt cgatcgcgac gatgatcacg cccgcaatgt cgagatagcg
      961 ctgcgcccgg tcgcgctcct cgcgcacggc gttctccgcc ttcttccgcc ggttgatgtc
     1021 gctgaagaaa agcagcgccg cgcgtccctg ctcccagtcc accgccacca cgttcacgaa
     1081 cagccacttg atcgccccgc ccttctcgat aatgcggaac tcgtactggt gcgccggctt
     1141 gttccgccgg gtgtacttga gataccggtc gccgaccggg gtccggtcgt ccggatgaat
     1201 caagtccatc acgggtatcg cggagatctc ccgttccgtg tagccggtga tctcgcacgc
     1261 cttggggttg gagaaccgta taaaaccgtc ctgcaccacg atgatgccct cgttggcgtt
     1321 ctccacgacg agccgatagc gctcctccga gagctggaag gcccgttcca cgcgccgccg
     1381 ctcggtgatg tcgatcgcgg cgccctcgat atagcccgca tcgccgaaca gtcgcgcggt
     1441 taccgaaagc caccgcagcg ccccgtcctt cgcgcgatac tcggactcga aatcccgcac
     1501 ggtcccgctg gagcgcaatt cgcgcagcat atcatcacga cgatcagggt ttacgtaatg
     1561 atcgaggaca acgcaggtcc cgatgcagga ctcgcggcgt tcatacccga gcagccgggc
     1621 gaagcgatcg ttgcattcaa tcacgcagcc gtcgtcgata cgggtgcggt acaggcccac
     1681 ctgcgcgttc gtaaacaggt cgcgatactt ctcctcgctc gccttgatcg cctcctcggc
     1741 acggacgaga tcggtgacat cgagcgcgtt caccaccagc tcgtccgcgt tcatcctggt
     1801 gcacctgatg cggcaccagc gcgcgtcccc gctccccgtc acgatgggat agatgcggtc
     1861 cggcgagttg tcggcatcgc gatcgtaaag ccagttggcg accacggttt gccggacctc
     1921 ggggtcgggc accacgcgat cgatgatctc cgcgaggttc ccgaagtccg ccgcggtgta
     1981 tccgaagatg cgctcaattc cgggactggc gtaccgcaag acgccgtccc gcccgatcac
     2041 ggcgatgccg tcgctgctgt aacgaacggc cagcgagtgc aaccgcgccg cctgggccac
     2101 cgcccggttg cggatatact ggcggtcgag gaacgcgcca agcccgataa aaacgcacga
     2161 gatcgcaaga tggaccagga tgaggaaatc gccccgccag gacaatccgc tggccgcgag
     2221 aaggtatttg atgaaaaact catagctgag cacggcgccc gataagtacg cgagcgcaac
     2281 accgatggcg acgacgagga cgatgtggga tcggagccaa ttcatgatcg tttcatcgct
     2341 ggattgctgc gccatccagc gggacaggcg gagccaccgc gccattcggc gcggcatcgc
     2401 caacgccccc taagaacatg acgtggcagc tctacgaata aatccggacg acgggacttt
     2461 ttattcccgg ataatcttcc tgtcaaccga aacgcggtcg ctatcgtgat attccacgaa
     2521 agcggcggcc ccggggttct ccttgaagcg ttggacgttc agcatgaaga tttccatccg
     2581 gttgatgacg ttcggcggca acagattgcc gtcgatctcg atggacatca ccggatactt
     2641 ccgctcacgc gcccacggcg tgatcagccc ctcgatgacg cgcccgatga ggcaggcgaa
     2701 gggcgagatg ttgacgacgc cggaatagcc gtcctgcatc gccgccgacg ccaccccgct
     2761 cgaaatggag atctccgagt agagctcggg ggtcacgaag atcgtgttcg cgttcttcat
     2821 gatcttcttc atgtcgtgcg gggtctccgg gatgaggccc gtggccgcaa gcgccttttt
     2881 gacggtgtgc tccacccggt gcttgtacca ctgctcgacg tgccagttga tcagatcgcg
     2941 gaagtccttc gagaaatagc gccgatagaa gggaatgagc gagaggcgtt tcttcagctc
     3001 gaaatggcgc gtgtagtcgc agtagtagat ccattcggac acgccggaca ccttgccgat
     3061 gatgcccttc tcgctgaagc ggcgcaccat ctcgtccacg gcgaaatcgt cgcggcgcac
     3121 gtagatctcg cccacgatga gcacgcgcgg gcactcctcc tgtttccgct tgagcgggat
     3181 cttccggatc tcgcgcgcga actcctggag gtgcggcacg atcttggtca cgtcgccctc
     3241 ggccacgccg atcatcttgt gccacagcgc gtcgtagcgg tccatcgcgg ccaccgggtt
     3301 ctccgcgcag gagcgcagcg aggtctcgat gtccttcatg tagtcgctga tgatgacgcc
     3361 ccaccaggcg aacttcgaga aattgggacc gagctcggtg taggaattat ccgaatccag
     3421 cgtgatcatc aacacgttct cgatgcgcag gtcacggaag aggttctcgt aaaagacgaa
     3481 gtactgcccc gtgcggcagg ggccggtggt cgtgggaacg aacaggagat acacctcgtc
     3541 cttgcgatac ttgtcggagg cgaggaactt cagggcgctc cccagcacca gaagcgacgg
     3601 caggcattcc ttgcccgagg cgtggttgcg cgccagctgc agcgtgtgga tgtccggcac
     3661 cggcatggcc gatgcgttga tcccggccga gcggaccgag gccgctaaaa gctccacgcc
     3721 cagccggccc atgttcgcca gcaggagctt cacccgcgga ttgtccttga tcggaataat
     3781 ctcattggtg acggcgttct tgatatagaa atccttgccg cgctcgttta tgaagcgcag
     3841 gccgttgtcg tagcgctccg ccgcgatatc gccgagcttg gagcggtagc cctcgatgat
     3901 atccaggaag gcctcaaccc gcgtgtccac gcccgcgtcg gcggtgtgcg aatcgagctc
     3961 caggatcagg aacggcttca cgcccatgat ccacttgaga taatgcagca tgaacgaatc
     4021 cggcgcgcac gagaagttgg agatataggt cacgaagata ttgtcttcct tcttgaggaa
     4081 attcgccgcc ttcatgtcct gctgcccgta gaaccagtac atgttcttga agatgagctc
     4141 gttctcgaag ggcaggatgt cgaacgggac caccgagtag ccgcgcgtgg tgaacttctt
     4201 gggaatgccc atgttcgcgt ccggagtgaa ggcgttgtag ggccggccga ggaccgcgat
     4261 caccggccgc ttcgcgatgc gcgcctcctc gatcgcccgc ttgccgagat cgcggatctt
     4321 gtggatgaac tcgatctgct tctcgtaggc cgtctcgaag gccttacgca catcgccgtg
     4381 cgacacgccg agattgtcgc ccagctcgag aaaggactcg atggccttgt cgaggccgta
     4441 gtgaaaggtg atcaccggct tcaggaactt cgactcgggc acgtcgggga aggccttggc
     4501 gatatagtac ggcaggctct gggtgatggg gcagaaattg gcgatgagcc cctcctcgta
     4561 accctccagg tcgcggaagt gcgggacgaa gatatagtcc gtaccgcggt ccacgatgtc
     4621 ctgcaccgcg ccgtgcgcga tctcggcggg gaaacagtag gcgctctcca cgcgcgcgac
     4681 cccttcgtgg gagatgttct tcgagaggac cgcctcgatg cccagcgaat ggaaaaacca
     4741 cgagtagaac ggccacatcg tgtacacgga aaagcaccgc ggcacgccca ccgtgacgtc
     4801 ccgcttcttc acgaaggtcg ccgggtcggg cgcgcactcg acgaacagca catcgttgcg
     4861 cgcgtcgata taattgaata cctcggcctc gtcgaaggcc tttttcttcc gaatgttcgc
     4921 atacttgttg cagcgccccc cgaacatgta ccggtgctcg ttcaccttga gcacgcggat
     4981 gggacagtaa ttctcgcagg cctggcactt gaattcgcgc tcgtagacga tctcggtggc
     5041 caggatctcg tcgatgtcgt acgtcgtctt ctcgaggaac ccgtcggcga gcttctgttt
     5101 cgcgagcaat cccacgccaa agcagcccat gagctccggg tccggcggca cgatgatggg
     5161 cttgttcaga aggagcgcga aggccagcgg gatggccttg ttcttcgcga cgccgccctg
     5221 cagcacgatc ttgttgccga tggtccggtt gcccaccacc cggttgaggt agttggacac
     5281 gatggaggtg accaggcccg cggtgatgtc ctcgcgcgtg gcgccctgct ggatcgcctt
     5341 gcgaatgtcg gagttgatga aggccgaaca gtgctcgccg aacttgagcg gctcgtcggc
     5401 catgatggcg atatcgccga tctcccaggc gtgctcgatg gagagatcgc cctgcgccga
     5461 ctcctcgagg aaggagccgg ttcccgccga gcaggcctcg ttcatcgcgt agtcgatggg
     5521 aacgccgttc ttgagcagca cgtacttcgc gtcctgcccg ccaatctcga agagcgtgtc
     5581 gatgtcgttc gcgtaaaagg tggtgcccac tgaatgggcg atgatctcgt tatagacgcc
     5641 cggcgtctcg agaaacaccc cgaggatctc ccgcgacgag cccgtggtgg ccgcgaggct
     5701 gatggagatg ggggcgtcgc cgatgtcttc cttaatctgc ttcttcatct cgacgaggca
     5761 ctccttgagc gcctgtacgg gatcgccgtg ggtgcggccg tagaagttcg cggtgatctc
     5821 gttggtctcg atgtcgatga ggcaggcctt cgtggtggtg gagccgccgt ccacgcccag
     5881 gacgtactcg cggttcggcc gcaccttcgt catcttggag gggatgtagg tcaccttccc
     5941 ctcggcggtc ttgaggctct tgaagcgctc gaagacgacg cggttctccc tgaagagttt
     6001 tttaagcggc ggcagcacgg tgcctatgga gcgcgcgagg agccccgccc cataggcctc
     6061 gaagtaggcg gcgttgtcgg gcaccacgaa ggtcagctcg ggcaggcgcc ggcggatata
     6121 gtccacgatg tatttatttc gcgtaacgcc gcccgcaagg agcacggcgc cgccgccgat
     6181 ccgcgcgcgc ttgaggaagt ccaccacctt ggtggccatc acgtcggaga gcgagaggac
     6241 gatgtcgccc ttggatgcct ccttcttgtt gaggcggtgg gtgcagtcgc tcttcatgaa
     6301 caccgagcag cgcgacgaga gcttgtgcac cgtgcacccc tcggggatgc cgttgatgtc
     6361 gtccaggcgc atgtccattc gcccgagctg ctgcttgaag aactcgcccg tcccggaagc
     6421 gcacttgttg ccggagaagc tgttgacgat tttcccgttc gcgtcgatgg tgtagacgac
     6481 gagatcctcc ccgcccatgg acacgacggc gtcgaccttc gtgttcacgc gcgcgagcgc
     6541 ctcctcgacg cacagcggct cgatcacgct gttcacggtg agcaggtgcc ggccctcgat
     6601 gcccgtcacc agcgtcttca ggtcggcggg gaagtccttg ccctgcatga cttttctcag
     6661 cgtatcgaag atattgccct cgtgcgcaac gacctcgctc cagaggatcc cgtttccctc
     6721 cagcatggcc accttcacgc tcgacgaccc tatgttgatt cccaggctat gcatgtgcgc
     6781 ctccgcgctt tgttttcgcc gccggccccg cagcgctata caaatatgta ggaaccggcg
     6841 cgcaatgtaa tcacgaatgt ttcggccaag gaaaatgtca aattaattat cccggcgcag
     6901 cgcggcgcgc agcccggcgg tcggcccctc ccggcgcatg gaccgatgcc cttaaaagtt
     6961 cgtatcttcg cacgctcatg tgccgataga aataagtgag gaaaacgcgg gcgcagggcc
     7021 cgcacgggac cgacgcgcat acaaccacca cgaggaagcg attcgccatg gaacatatca
     7081 tacaaatact ttcggagatt cccatctttc aggggctcag ccgcgaggac ctcgcgctca
     7141 tcgtgccgat cctgtcgaca cacgagctcc cggccgagag cctggtcgta aaggagggcg
     7201 acatcgggga ctcgatgttc atcatcaagt cggggacggt gcgcgtcacc aagaccgagg
     7261 agaacgggtc cgaggtgctc atcagccacc tctacaaggg gcagtacttc ggggagttct
     7321 cgctcatcga caacctgccg cgctcggcga acgtggtcac cgaggaggcc acggagatct
     7381 tcacgctcaa ccggggcgac ttcgaccggc tgctcgctgc caacatagta atcaaggcgg
     7441 ccttccacca gaactgcctc aaggagacct tctcgcggtt ccggaacatc atctccaatt
     7501 tcaccttcgc gcagcacgac ctgcgcgaga agcgcgaggc gctcgacgag atcaaccgcg
     7561 acctgtcgct ggccaagaag ctccagcgct atttcatcaa caccgactac ctgcgcaccg
     7621 agcacaccgc ggacaacggc gtgcggcact cctatatcta caagccgtcc atcgcgatcg
     7681 ggggcgactt cctgaacgtc ttccagctcc gtgacggcgt ggtggggatg atcatcgcag
     7741 acttcgaggg ccacggcatc accgcggcgc tcgcgacggg cgtgctcaag agcgcgctct
     7801 ccatcgtgct tgcggacctg ggcgaaaagc ccatcgagct catgtccttc ctcaacgacc
     7861 atttccacaa ggtgatcgcg cagctctacg cgacctgcta ctatgcggtg atccacaccg
     7921 aagagcgccg gatcgtcctg gccaaggccg ggcaccacca cccgctcgtc tggaaagcgc
     7981 gcctgaacga cttcacgccc atcgagtgcc cggggacggg gctgggactc atgcccaatg
     8041 cgcgcttcgg ggtcgtggag caggacatcg agcccggcga caaggtgctc ttctttaccg
     8101 acgggatcat cgagcagatg aacggcttcc acgagatgta ctcgaagaag cgcatgacgg
     8161 agaaggtcc
//
LOCUS       DAORHF010000170         8150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8370_length_8150_cov_5.237554, whole genome shotgun sequence.
ACCESSION   DAORHF010000170 DAORHF010000000
VERSION     DAORHF010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8150
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8370_length_8150_cov_5.237554"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKJ16_13085"
     CDS             complement(<1..449)
                     /locus_tag="PKJ16_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47971.1"
                     /translation="MTNKHKGSLIAFVAISAAAYAIFLAVALLRTGGAHVWITESWQI
                     RSQDSPFIFAALCLTQYIWGPLLGLALGLFLVYWLFKKGLAFVMVGVFIIAMTGVFAY
                     FSLSKLADPLGKGLVDLIGISALCLFLLAAGVAMFVGHVKMRRQISGA"
     gene            complement(566..1096)
                     /locus_tag="PKJ16_13090"
     CDS             complement(566..1096)
                     /locus_tag="PKJ16_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47972.1"
                     /translation="MKALTNASAALAIFCAAALLIVRVIVSGATVGPITITGIATEME
                     DYRIIAERGREATAVVTGVTTPRRTKITDKIRHSVSARFTAGANGIDTEYWIDDDDMR
                     ALFAGPAVGRTLAVRYDPEDPRRSAPAFTFTARGASMAENADLFALLRRGFAIACAVF
                     TGVFIASIAVRVIKKS"
     gene            complement(1158..2306)
                     /gene="alr"
                     /locus_tag="PKJ16_13095"
     CDS             complement(1158..2306)
                     /gene="alr"
                     /locus_tag="PKJ16_13095"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV47973.1"
                     /translation="MQLHIETYKNRPTRAEISLPNLRNNFAVVRSIVAPGVKIMAVVK
                     ANAYGHGIHEISKELLASGADYLGVAYLEEALYLRRRGITAPILVMGAINTDQIAEFL
                     ENDIEITSSSYDKSAAISAAAVRAGKDALVHLKVDTGMERIGVHWYNAPAFIEKTFSL
                     PRIRVTGIFSHFAKSESDPAFTKTQLERFDSVLDFCAHKGLTPGLVHIANSGGIINHP
                     ESHYTMVRPGIMLYGYNPNGHLPEVRFGERALRPVMSLKTKVAYFKVVPPDTGISYNH
                     AYRTKEQTRIVTLPAGYGDGYSRLLTNRGEVVIRTRRHPLVGTICMDQMMADIGPDGV
                     AYNGDDALLFGEMDGVSIPLESLCGKIGTITYEVLCGISARVPRIYLQ"
     gene            complement(2355..3635)
                     /locus_tag="PKJ16_13100"
     CDS             complement(2355..3635)
                     /locus_tag="PKJ16_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47974.1"
                     /translation="MTLECHQCSSTDLEKTSPREYRCRHCGAITIVTVPEDVVDAYEI
                     RQAKQRALRKKIALLTIAGGVIVFAILTAYGAIFTLARSHTLASREIKRFGSPRSGHG
                     RMIPSMTGAIFDDHSMIARPNAIRAMSLSRDGKSLATMAEKDIIVWNVADGSVIAHAD
                     RSTLQAEGYFRDICWIEFFPDGKRIACAPDYGRVLILDAKTLNPIHRISLPHESELRA
                     GRIVHDGTVLALTETRIDVDRYVILYAIRGSTISARVKMKSDIDSVFSGAISPDGSMI
                     AFPQHKSLAVCDTENGRVITAFEAVPAALGSIAFGPDGARLAYQVLLRDKSSNEESVL
                     FVRDIESGDVVARIRHDSEASGWSIAFSPDGRLICPMPEAKRMRIYTTADGAPLRTLS
                     PIGTSYAIHFTHDGKSVITGGEDMLVRFWRVPER"
     gene            complement(3632..4897)
                     /locus_tag="PKJ16_13105"
     CDS             complement(3632..4897)
                     /locus_tag="PKJ16_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNV47975.1"
                     /translation="MPLHCRQCAGTDLEQVSPDEFRCRYCGTITFIEPTIEEAERRRA
                     ERTASARKSRHPVRLALAVIFGGIIIFLLGSVAATWIKLRTLDADEIKRLGSLAGGHV
                     RILPAVAGLFMDDPPHVIDRNGITALCLSADGKTLYSGAHDDMIAWNVESGGIIKQIH
                     FREWGFLYGSAVAIVHLPKRREIAVLFHSTDSHVLFLDERTLAENARTVSNDSILHIT
                     ARPDDRVVALVLERRSDAEYSFRLGVGTVGTKGMILRHRSESYPHTQMGAVLHPFGSL
                     YAAFAPDGETIIVRSATDGEEMFCVRHQNDNRRYLAFSASGKHFIYTNNDSLIIRDIS
                     DFTIIADIPLDQTFYGFDMRCSVSDDGARAFFFDWSTRTITVRRLPGGAMLPPVRGSG
                     LFSTAIFTPDGKTLITGGTDQLIRFWRAP"
     gene            5026..5868
                     /locus_tag="PKJ16_13110"
     CDS             5026..5868
                     /locus_tag="PKJ16_13110"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HNV47976.1"
                     /translation="MEKQETEQYEKIESGKFGFFSKPYLATAGPDAGKIIKQYHHIKK
                     RALCEELVSIHDEYFALLRKAGISVPDTEIRLVAEKGGAHTIRIVQSAFPEAALVRNI
                     MQATDFPLCERLMRGIIDDAVTVLVFMRENPKENMGFHPTLRNYSFHDERLHYFDTFP
                     PFCRWGQRRVEEIILEFAPFGFVKYVKPLFKPFMHRVTDEYYFEDKMVLGIVGSSCRL
                     RPEFYQRFLDSAKEHVGAVPDARLLQKQAILDQLSQPPSLSPIWVFTRRMLGKEGQPN
                     LKVK"
     gene            5888..7063
                     /locus_tag="PKJ16_13115"
     CDS             5888..7063
                     /locus_tag="PKJ16_13115"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV47977.1"
                     /translation="MMQAHGGSASKRMTIEKAAGTDGGRNAFPIGRILAIGIALFVAI
                     FAGVIAFMLLSDRMILRSPVLVLSRPRVTFALGESSYRERADGPWRRVTIGSAIGEGC
                     EVKTGPGALLDLHLHDGTTVRIMQNTHAKLLRHVIKELHVEVSGGSLYGRFKKIFTEH
                     QMRISTPTSTVAVRGTEIGVEVARIAPPAVPKEKPARRGRASRAAVPAPVPVETVVST
                     TVYGLSGVVEVVNPLFPDRPVLLGNRNMVRVVASAPPSDPERMSENDIIRVQTIINAL
                     HDEEVLLITNRIYFETDSAAILANSHPELDRVVALMNKRRAKVRIEGHTDITGSASIN
                     LPLSRRRADSIRQYLIAKGIDPKMLMIAGYGSGKPIADNRTPEGRAQNRHVEFIVIE"
     gene            7082..7543
                     /locus_tag="PKJ16_13120"
     CDS             7082..7543
                     /locus_tag="PKJ16_13120"
                     /inference="COORDINATES: protein motif:HMM:NF021487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HNV47978.1"
                     /translation="MNIESVLGVLKALRDHGVAYVLVGGLAVALHDVPRITEDVDIFV
                     RDDEQNVGKLRSALHSVYRDASIDEITKADMDAYAVIRYGTPDDYYIDIMSRIGEMFS
                     FEDVEFEVVAFKGVPVNLATIDALIMLKNGTLRDKDRADVILLREKQKEKR"
     gene            7543..7755
                     /locus_tag="PKJ16_13125"
     CDS             7543..7755
                     /locus_tag="PKJ16_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47979.1"
                     /translation="MPVYKYKTFAHAEAHLQELLPSDPAEKIMRMQNMLRIRGAAVSV
                     QRGVHKFKSFAEAEEHRKGSSGSSSS"
     gene            complement(7769..>8150)
                     /locus_tag="PKJ16_13130"
     CDS             complement(7769..>8150)
                     /locus_tag="PKJ16_13130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV47980.1"
                     /translation="MPIETDFAAIPPVQGDPNLLKIVVTNLVSNAIKYGKAGTPIKLR
                     LEKQDGAVVFSVHNEGVGISKEDIEKKLFSKFGRLKQKGTEGVKGSGLGLYICKKIVE
                     KHSGKIWVDSEAGSWVRFNVSLPA"
BASE COUNT         1570 a         2367 c         2572 g         1641 t
ORIGIN      
        1 gcgccactga tctgcctgcg catcttcaca tgccccacga acatcgccac tcccgccgcg
       61 agcaggaaca ggcacagcgc gctgatgccg atgaggtcca ccaggccctt gccaagcgga
      121 tcggcgagtt tcgacaggct gaaataggcg aacacgcccg tcatggcaat gatgaacacg
      181 cccaccatga cgaaggcgag tccctttttg aagagccagt agacgagaaa aagccccagc
      241 gccaggccca ggagcggtcc ccagatatat tgcgtcaggc acagcgcggc gaagatgaac
      301 gggctgtcct gcgagcggat ttgccaactc tcggtgatcc agacatgcgc gcctcccgtt
      361 cggaggagag cgacggcaag gaagatggcg tatgcggccg cgcttatcgc cacgaaggcg
      421 atcagcgatc ctttatgttt gttcgtcatg tttgcagcaa ccgttcgctt ggagcggtag
      481 gagcgcagcc gtcaatgctc gttgcgccaa caaatgcggg tgcgtgtatg ccgggagagg
      541 gcatggcgaa ttcggcgcgc ttcggttacg atttcttgat tacgcgtacg gcgatggacg
      601 cgatgaaaac gccggtaaag accgcgcacg cgatcgcgaa accgcgccgt aagagcgcga
      661 agaggtccgc attttccgcc attgacgcgc cccgcgctgt aaacgtgaat gcgggcgcgc
      721 tccgacgcgg gtcctccggg tcgtagcgga cggcaagggt gcgccccacc gcgggaccgg
      781 cgaacagtgc gcgcatgtcg tcatcgtcga tccagtactc ggtgtcgatg ccgttcgcgc
      841 ccgccgtaaa tcgcgcggaa accgagtgtc gtattttgtc ggtgatcttc gtgcgtcggg
      901 gcgtcgtgac gcccgttacg acggcggtcg cctcccgacc acgctcggcg atgatccggt
      961 agtcctccat ctccgttgcg atgccagtga tggtaatcgg ccccaccgtt gcgcccgaaa
     1021 cgataacgcg aacgatgaga agcgccgcgg cgcaaaagat ggccagcgcg gccgatgcgt
     1081 tggtgagtgc tttcatgctc ggttcctccg tggtgccgtc tctgtgtctt cgcttgagcg
     1141 tacgcccggc gcgtcattca ttgcaggtag atccgcggga cgcgcgcgct gatgccgcag
     1201 agcacctcat aggtgatggt gccgatcttc ccgcacaacg actcgagcgg gatcgatacg
     1261 ccgtccatct ccccgaagag aagcgcgtcg tcgccgttgt acgcaacgcc gtcgggcccg
     1321 atgtccgcca tcatctggtc catgcagatg gtgccgacca gcggatggcg ccgcgtgcgg
     1381 atcaccacct ccccgcggtt ggtgagcagc cggctgtatc cgtccccgta gccggcgggg
     1441 agcgtcacga tgcgtgtctg ctccttcgtt cgatacgcat ggttatagct gatgccggtg
     1501 tccggcggca ccaccttgaa gtacgccacc ttcgtcttga gcgacatcac gggccgcagc
     1561 gcccgctcgc cgaagcgcac ctcgggcagg tggccgttcg ggttgtagcc gtagagcatg
     1621 atgcccgggc gcaccatcgt gtagtgcgat tcgggatggt tgatgatgcc gcccgagttc
     1681 gcgatgtgca ccaggcccgg cgtgagcccc ttgtgcgcgc agaagtcgag gaccgagtcg
     1741 aagcgctcga gctgggtctt cgtgaaggcc gggtcgctct cggatttcgc gaaatgcgag
     1801 aagatgcccg ttacccggat ccgcggcagc gagaaggtct tctcgatgaa ggccggcgca
     1861 ttgtaccagt ggacgccgat ccgctccatt cccgtatcga ccttgaggtg aaccagcgca
     1921 tccttcccgg cgcgcacggc cgccgccgag atcgccgccg acttgtcgta tgaggagctc
     1981 gtgatctcga tatcgttctc caggaactcg gcgatctggt cggtgttgat ggcgcccatc
     2041 accaggattg gcgccgtgat gccccgccgt cgcaggtaca gggcctcctc gagataggcc
     2101 acgcccagat agtccgcgcc ggacgctagt agttcctttg agatttcgtg gatgccgtgt
     2161 ccgtacgcgt tcgccttgac cacggccatg attttcacgc cgggcgcgac gatggaccgc
     2221 acgaccgcga aattgttccg caggttcgga agcgaaatct ccgcgcgcgt ggggcggttc
     2281 ttgtatgttt cgatgtggag ctgcattgct ttgtccgcaa gtaagaagcg ggcccggcgg
     2341 gtctgtcaat tgttttatcg ctccggcacc cgccagaacc gcaccagcat gtcctccccg
     2401 ccggtgatga cgctcttccc gtcgtgagta aaatgaatgg catagctcgt gcctatgggg
     2461 gagagcgtcc gcagcggcgc gccgtccgcg gttgtgtaga tgcgcattcg tttcgcctcc
     2521 ggcatggggc agatgagccg gccgtcgggg ctgaaggcga ttgaccagcc gctcgcctcc
     2581 gaatcgtgcc gaattctcgc gacgacgtca ccgctctcga tgtctctgac gaaaagaacc
     2641 gattcttcgt ttgaggactt gtcccgaagc aggacctgat acgcaagccg cgcgccgtct
     2701 gggccgaagg cgatcgaacc gagggcggcc ggtacggcct cgaacgctgt gatcacgcgc
     2761 ccgttttccg tgtcgcagac ggcgagggac ttgtgctggg ggaatgcgat catcgaacca
     2821 tcggggctga tcgcgccgga aaacacgctg tcgatgtcgc ttttcatctt tacccttgcc
     2881 gatattgtgg aaccgcgaat tgcataaagg ataacgtacc ggtccacgtc gatccttgtc
     2941 tccgtgagcg caagcacggt gccgtcgtgt acaatgcgtc ccgcacgtaa ttcgctttca
     3001 tgcggcaggc tgatccgatg gatcggattc agtgttttcg cgtcgaggat gaggaccctg
     3061 ccgtagtcgg gcgcacacgc aatccgtttg ccatcgggaa agaactcgat ccagcagatg
     3121 tcgcgaaaat atccttcggc ctgcagcgtt gatcgatcgg catgcgcgat gaccgatccg
     3181 tcagcaacgt tccatacgat gatgtccttt tccgccatcg tggcgagcga tttcccgtcg
     3241 cggctcagcg acatggcgcg gatggcgttg ggccgcgcga tcatcgaatg atcgtcgaag
     3301 atcgcgccgg tcatcgacgg gatcatgcgg ccatgaccgg atcggggaga tccgaagcgc
     3361 tttatttcgc gcgatgcgag ggtgtggctt cgtgcgaggg tgaatatcgc gccgtacgcg
     3421 gttaggatcg cgaacactat cacccctccc gcaattgtaa ggagcgcgat ttttttgcgg
     3481 agtgcgcgct gttttgcctg cctgatctcg taggcgtcga cgacgtcctc gggcaccgtc
     3541 acgatggtga tcgccccgca gtggcggcag cgatactcgc gcggtgatgt cttttcaagg
     3601 tccgtgcttg agcattggtg gcattcgagc gtcatggcgc cctccagaag cgtatgagct
     3661 ggtccgtgcc gcccgtgatg agcgttttcc cgtccggcgt gaagattgcc gtggagaaca
     3721 atcccgatcc gcggacgggc ggcagcatcg ccccgcccgg aaggcggcgt acggtgatcg
     3781 tccgggtgct ccagtcgaag aagaacgcgc gggcgccgtc gtccgatacg gagcagcgca
     3841 tgtcgaagcc gtagaaggtt tgatccaggg gaatgtcggc gatgatggta aaatcagaga
     3901 tgtcgcggat gatcagggaa tcgttgttcg tatagatgaa gtgttttccg ctcgcgctga
     3961 aggcgaggta gcgcctgttg tcgttttgat gccggacgca aaacatttcc tcgccgtccg
     4021 tcgcggagcg tacgatgatc gtttctccgt ccggggcgaa ggccgcatag agcgatccga
     4081 aaggatgcag gacggccccc atttgagtat gcgggtatga ttccgagcgg tgccggagga
     4141 tcatgccctt cgttcccacc gttccgactc ccagccgaaa ggagtactct gcgtcactcc
     4201 tgcgttcgag caccagggcg accacccgat cgtccgggcg cgcagtgatg tgaaggatgc
     4261 tgtcgtttga taccgtgcgg gcattctccg cgagtgttcg ctcgtcgagg aagagcacat
     4321 gggaatcggt cgaatgaaac aaaacggcaa tctcgcgtcg tttcggaaga tgaacgatgg
     4381 ctactgcgga accgtacagg aatccccact cgcggaaatg tatctgtttg atgatgccgc
     4441 cggattccac attccatgcg atcatgtcat catgcgcccc cgagtagagc gttttcccgt
     4501 ccgcgctcag gcacagcgcg gtgatgccgt tccggtcaat cacatggggc gggtcgtcca
     4561 tgaagagacc ggcgacggcc ggcaggatgc gcacatggcc gccggcgagg cttcccaggc
     4621 gcttgatctc gtccgcgtcg agcgtgcgca acttgatcca cgtcgccgcg accgatccga
     4681 gaaggaagat gataatgccg ccgaagatga ccgcaagagc gagtcggacg gggtgacgac
     4741 tcttccgcgc agacgccgtg cgctcggcac ggcgccgctc cgcctcctcg atggtgggct
     4801 cgatgaaggt gatggttccg cagtagcggc agcggaactc gtcgggcgac acctgctcga
     4861 ggtcggttcc ggcgcattgt ctgcagtgga ggggcattgg cgctctatgg aaaatcatca
     4921 aaatagtatt tgagcgccca agccgtgtca attagtaatt gtatttttct cgatgggtta
     4981 tagaaacggc aacaagacga gaccatcgag agcgaatcac ttcacatgga aaaacaagaa
     5041 accgaacagt acgaaaagat cgaatccggc aagttcggat tcttctccaa accatatctt
     5101 gccactgcgg gtcccgacgc ggggaagatc atcaagcagt atcatcatat taagaaacgc
     5161 gccctgtgcg aggagctcgt ttcaattcac gacgagtact tcgcgctgct gcggaaggcg
     5221 gggatatcgg tccccgacac ggagatacgc ctggtggcgg agaagggcgg ggcgcatacc
     5281 atccgtatcg tccagagcgc cttccccgag gcggcgctag tgcgtaacat catgcaggcg
     5341 accgactttc cgctctgcga gcgcctcatg cgcggcatca tcgacgacgc cgtgaccgtg
     5401 cttgtcttta tgcgggaaaa cccgaaggag aacatgggct tccatcccac cctgcgcaat
     5461 tattcctttc acgatgaacg gctgcactac ttcgacacct ttccgccctt ctgccgctgg
     5521 ggccagcgcc gggtggagga aatcatcctg gagttcgcgc ccttcggttt cgtgaagtac
     5581 gtgaagccgc tcttcaagcc gttcatgcac cgcgtgaccg acgagtacta cttcgaggac
     5641 aagatggtgc tgggcatcgt ggggagctcc tgccggctgc ggccggagtt ttatcagcgc
     5701 tttctcgatt ctgcaaagga gcacgtgggc gcggtgcccg acgcgaggct gcttcagaag
     5761 caggccattc tggatcagct ctctcagccg ccgtcgctga gccccatctg ggtgttcacg
     5821 cgacgcatgc tgggcaagga agggcagccg aatttgaagg tgaagtagga ctagcgcgga
     5881 ggatcgcatg atgcaagccc acggcggatc ggcgagcaaa cgaatgacga ttgagaaggc
     5941 ggctggaacc gatggcgggc gaaacgcgtt tccaatcggt cggattctcg cgatcgggat
     6001 cgcgctcttc gtcgcgatct tcgcgggcgt catcgccttc atgctcctgt ccgatcgcat
     6061 gattcttcgc tcgcccgtgc tggtcctgtc ccgcccaagg gtcaccttcg cgctcggcga
     6121 gtcgagctat cgcgagcgtg cggacgggcc gtggcggcgt gtcacgatcg ggtcggcgat
     6181 cggcgagggg tgcgaggtga aaacgggccc cggcgcgctt ctcgatctcc atcttcacga
     6241 tggaacgacc gtccggatca tgcagaatac gcatgcaaaa ttgctgcggc atgtgattaa
     6301 agagcttcat gtcgaggtga gcggcggatc gttgtacggt cgcttcaaga agatcttcac
     6361 cgagcatcag atgaggatca gcacgcccac gagtacggtc gccgttcgcg gcaccgagat
     6421 cggcgtcgag gtcgcgcgca tcgcgccgcc tgcggtgccg aaggagaagc cggccagaag
     6481 agggcgcgcg tcgcgagcgg cggttcccgc gcccgttccc gtggaaacgg ttgtttcgac
     6541 aacggtgtac gggttgagcg gcgtcgtcga ggtggtcaat cccctatttc ctgaccgtcc
     6601 ggtgctgctc ggaaaccgaa acatggtgcg ggtggtcgca tcggcgccgc cgtcagatcc
     6661 ggaacggatg tccgagaacg acatcatccg ggtgcagacg atcatcaacg cgctgcacga
     6721 cgaagaggtg ttgctcatta cgaacaggat ttacttcgag acggacagcg cggccatcct
     6781 cgcgaattcg caccccgagc tcgatcgcgt cgtcgcgttg atgaacaagc ggcgcgcgaa
     6841 ggtgcgcatc gaaggccata ccgacatcac gggctccgcg tcaataaacc tcccgctctc
     6901 gcgacgccgg gccgattcga tacgccagta tttgatcgca aagggtatcg atccgaaaat
     6961 gctcatgatt gccggatacg gctccggaaa gcccatcgcc gacaaccgca cgcccgaggg
     7021 acgggcgcag aaccggcatg tcgagttcat cgtgatcgag tagagggggc gtcacatcag
     7081 catgaacatc gaaagcgttc tgggcgttct caaggcattg cgcgaccatg gcgttgcgta
     7141 tgttctcgtt ggcggtctcg ccgtcgcgtt gcacgacgtg ccgcggatta ccgaggatgt
     7201 cgatatcttt gtgcgcgatg acgaacaaaa cgtgggtaaa ctgcgatcgg cgctccattc
     7261 cgtatatcgc gatgcgagca ttgacgagat cacgaaagcc gatatggatg cctatgccgt
     7321 gattcggtat ggcacgccgg atgattacta tatcgatatc atgagccgta tcggagagat
     7381 gttttctttc gaagatgttg agtttgaggt cgtcgcattc aagggggttc cggttaacct
     7441 cgcgacaatc gatgcgctga ttatgctgaa aaacgggacc cttcgcgata aggaccgggc
     7501 ggatgtcatt ctgctgaggg agaagcaaaa ggagaaacgc tgatgccggt ctacaagtac
     7561 aagacctttg cccacgcgga ggcgcatctg caggagcttc tgccgagtga cccggcggag
     7621 aaaatcatgc gcatgcagaa tatgctgaga atacgcgggg ccgcagtatc ggtccagcgc
     7681 ggcgtgcata agtttaaatc atttgccgag gcggaggagc accggaaggg ctcttccggg
     7741 tcaagttcat cgtgatcgag taggagcgtc acgccgggag cgaaacgttg aatcgcaccc
     7801 acgagccggc ctcggaatcg acccagatct ttccgctgtg cttttcgact atttttttgc
     7861 agatgtagag gcccagcccc gaacccttca cgccctcggt ccccttctgt ttgagccgcc
     7921 cgaatttcga gaagagcttc ttttcgatgt cctcctttga gatgcccacg ccctcgttgt
     7981 ggactgagaa gacgaccgcg ccgtcctgtt tttcaaggcg cagtttgata ggcgtgccgg
     8041 ccttcccgta cttgatggcg ttgctcacca ggttggtgac cacgatcttg aggagattgg
     8101 ggtcgccctg caccgggggg atcgcggcga agtcggtctc gatgggcatt
//
LOCUS       DAORHF010000171         8026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8574_length_8026_cov_4.625643, whole genome shotgun sequence.
ACCESSION   DAORHF010000171 DAORHF010000000
VERSION     DAORHF010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8574_length_8026_cov_4.625643"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..949)
                     /locus_tag="PKJ16_13135"
     CDS             complement(110..949)
                     /locus_tag="PKJ16_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV47981.1"
                     /translation="MKLEKRFPERRVVITGAGSGLGRALALEFASRGWRVAVTDINMA
                     RLAETAAQVAAAGGSAREIACDVTKPADFARVLEAVRTGWGGIDVLVNNAGVAAGGFM
                     DRIPMDEWERIIDINLKSVIIGCRTFIPQFAAQGSGHIVNVASNAGIASLAEMSSYNV
                     TKAGVISLSETLRVELAPRGVGVTVVCPTFFKTNLMDGFRSSDERQRRLAEGMFAKAS
                     IGAGEIARHVVRSIERGRLYALAQKDARLAWRGKRLAPEAYFRFLGWAYRTGMFEKYA
                     GVR"
     gene            complement(951..2078)
                     /locus_tag="PKJ16_13140"
     CDS             complement(951..2078)
                     /locus_tag="PKJ16_13140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV47982.1"
                     /translation="MNAIINDMYVAERQAFADLARDFCSKRLIEGREERDRYPFGGLM
                     EEAIGDAAAVGFYGINSPVDYGGVEMNATMLASILERISREDAGMAAIIFANAAALEV
                     LRVAAEQTGCADVYGRIHSLGTAPIAFQSYAGPEECELPTVDAHSLMSGGVGFLALAP
                     IAEYAVIPARHGAGARYAYYLVALASEGVRVHDPIVSLGLRGCPAADVTFERVPATLI
                     GTGDGPGYFDSMRASLSIAAAAISLGILKGSLRDAMRYAADRYQGGRQIIDWGQVRMM
                     LANMAVEERIAEACLDTAYRELDSGMPGWQLTALAAAIHVGELAVRATADGVQLFGGN
                     GYTKDYPQEKRMRDARQAQCLLGMAPLRKMALVDRVIEERL"
     gene            complement(2096..3610)
                     /locus_tag="PKJ16_13145"
     CDS             complement(2096..3610)
                     /locus_tag="PKJ16_13145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV47983.1"
                     /translation="MKPWKIGRRERAALLEQGPTIREVFNGLECQHRLPAGAHREVAE
                     AIAIARKFTREVIRPIALDTDLRCMRDHNYLPLEFVKKAAEWGLFTLFIPKIFGGKGM
                     SFLAVYPFMEEISAGCAGLGHVIAVHYCGAATLFPSMNARIIRKVFADVAKSERAGDP
                     HLLTIVVTEPESGTDVQEPLLIHNARPGSSVRRVEGGYVLNGKKIFISNGHLSYWHIA
                     NFIEDKGSKTDSWIQFAIPNGTPGFSFGAHEKKMGQFACVASELLFDECFVPDSLVTI
                     RTQDPEFRDSRKGARWVCHTITDHVTGGTRTGTAAISTGIARDAYERALAYARTKQVG
                     GAPLIDRQWAQIILADMYRNVNLSRIMYLEAAFALTLSGLFKLLFKKPVYGVIRWLPR
                     WYFSLIAPLLDLRIATRFLRWYYYDGYPTEERNVASGWGSACKYTCSDLGLANANLAL
                     DLMGADGLRHENGAEKCFRDVKLQQIYESTNQINQLNMFYCMVAHACPEVELFK"
     gene            complement(3700..5406)
                     /locus_tag="PKJ16_13150"
     CDS             complement(3700..5406)
                     /locus_tag="PKJ16_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV47984.1"
                     /translation="MQNLIIKLKLAKALAAFASHELRRGTLARNLGSLVRSREIAEDM
                     SWAELLEERARMHPERPCIVFEGTCFSFRHMDRNANRVAHLLLSLGGGRARGVAIMMG
                     NCPAYLDVFIGAQKIGMYSVPVNTSLRGESLLYILNHSEAAFLAIDEDLLDVFIKIRN
                     RLIRQPVVIVNREPGSEQPPLPKGARSLSVATRLPASKPDGGYDTDDICFILYTSGTT
                     GLPKGVVYRYGKTTVKLLSIAAYALYRPSDVLYTCLPLFHGNALFLTITQGLHTGAKV
                     VLARKFSASRFWDDIRANGVTAFNTIGAMIPILMKQPERPDDRDNRVRLTISAACPAD
                     LWPQFERRFGVRIYEGYGAVDGGGKSIMNLGNAPVGSIGRPGPGVVYRLVDREGNDVP
                     VGMPGELIFAAKGERRSVEYFKNEKATGEKLRDGWIYTGDLVRKDEHGYLYFVGRNAE
                     FMRIKGENVSAYEVEHAIQRHPAVLEAAVYAVPSELAEDEIMASVSLVEGRSLSEREL
                     VEFLSEDLPRFALPRFVRIVEEFPKTETQRIRKKELERDGVLPGTYDMSMGRHIAESL
                     VS"
     gene            complement(5452..6903)
                     /locus_tag="PKJ16_13155"
     CDS             complement(5452..6903)
                     /locus_tag="PKJ16_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV47985.1"
                     /translation="MRDVYVIAVHTIKFGKYLDMGIKDLVAATVVPCLREAGIARRDL
                     AALWFANSGWGYARGQESIRGQVALRPLGIDGIPITNIENACAGGSTAFHHAWLGVAS
                     GLYDITMAVGAEKVHHHNKYKIFAGFLGGLDIENISRIIADLSRFGMDDDDRRRMAEH
                     VRRYAGSRAHRGGAETKARKGKNGAARYGRMRELGDMFRVYLQLSEMLGSDTVRDLRK
                     LLTGDHSPFMDVYGFAARWHMRKYGSTIEQLAAIASKNHLHSSLNPNAQYTFRVTPEE
                     VMADRLVSWPLTRSMCAPIGDGAASAVLCDGDTVRRLGLASRAVRVRASVLGSGRERD
                     FDDIDIGERLSRIAYEKSGLGPGEIHLAEVHDATAYGELHQAEALGFCAPGEGGVIAL
                     RGETSIGGRIPINTSGGLESRGHPIGASGLAQIHELVTQLRGEAGGRQVAGARIALAE
                     NGGGALGQEEAAMCIHILEAPSRGAGMATAGVQ"
     gene            7154..>8026
                     /locus_tag="PKJ16_13160"
     CDS             7154..>8026
                     /locus_tag="PKJ16_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47986.1"
                     /translation="MTPHTRIYHILLGMMLALTGSCANTDAPAPGTMDLSGQWELVET
                     VAGGPELPDYRATGAVPVTIPGRLDHIVGRTAEHAATVWLRKRVFLGRELAGSMLVLS
                     INNIGIADEAYVNGVCVGASGTFPAGGGTRGYDLAWHRDREYHIPAALLRPDRENLIA
                     IKVFFHVIDGVRERPSLSSARALHEANRFREFVPSANNLNPLILALLLYTLLIISMKG
                     AVNRRLAVYSNIFIMAVFGVTVLMLGLPRFDDNLYRFKLFLLLYVLADFSLILFIQEF
                     FSIRSRALTAGAVSV"
BASE COUNT         1349 a         2754 c         2488 g         1435 t
ORIGIN      
        1 ggatcacccg ctcgacgggg ttccagggca tgccctcggc gtaggtcccc gtgggggacg
       61 attcgttcgc atgaaggttc attcggttcc tccgtatgat ccgggcgcgc taccgcacgc
      121 ccgcatattt ctcgaacatg cctgtgcggt acgcccagcc caggaagcgg aaataggcct
      181 cgggcgcgag ccgcttgccg cgccaggcga gccgcgcgtc tttctgcgcg agggcgtaga
      241 gccttccccg ctcgatcgag cggaccacat gccgcgcgat ctccccggcg ccgatgctcg
      301 ccttcgcgaa catgccctcg gcgagccttc gctggcgctc gtctgaggac ctgaagccgt
      361 ccatgagatt ggtcttgaag aaggtgggac acacgaccgt caccccgaca ccgcgcggcg
      421 ccagctcaac gcgcagggtc tccgagagcg atatcacccc cgccttggtc acgttgtagc
      481 tcgacatctc cgcgagcgag gcgatccccg cgttcgaggc gacattgacg atgtgcccgg
      541 acccctgcgc cgcgaactgc ggaatgaacg tccggcaccc gatgatcacg cttttcaggt
      601 tgatgtcgat gatgcgctcc cactcgtcca tcgggatgcg gtccatgaac ccgccggccg
      661 cgaccccggc attgttgacc aggacgtcga tcccgcccca gcctgtccga acggcctcga
      721 gcactcgcgc gaagtccgcc ggcttcgtca cgtcgcaggc aatctcacgg gccgatccac
      781 ccgccgcggc gacctgcgcc gccgtttccg ccaggcgcgc catgttgatg tccgtcacgg
      841 ccacgcgcca tccgcgcgat gcgaactcaa gcgcgagcgc ccgccccagg ccgctcccgg
      901 cgccggtgat gacgacgcga cgttcgggga accgtttttc gagcttcatg tcacagacgc
      961 tcctcgatga cgcggtcgac gagcgccatc ttccgcaggg gagccatccc cagcaggcac
     1021 tgcgcctgcc gggcgtcgcg catccgcttc tcctgcgggt agtccttcgt gtagccgttt
     1081 cccccgaaga gttgcactcc gtcggccgtg gcgcgcaccg ccagctcgcc tacgtgaatg
     1141 gccgcggcga gggccgtgag ctgccagccg ggcatgccgg aatcgagctc gcgatacgcc
     1201 gtgtcgaggc aggcctcggc gatgcgctcc tcgacggcca tgttcgcaag catcatccgc
     1261 acctgccccc agtcgatgat ctgccgtccg ccctgatacc ggtcggcggc atagcgcatg
     1321 gcgtcccgca acgagccctt gaggatcccc agcgagatcg cggccgcggc gatcgacaac
     1381 gaggcgcgca tcgaatcgaa ataccccggc ccgtcccccg tgccgatcag cgtcgcgggg
     1441 acgcgctcga aggtgacgtc ggccgccggg cagccgcgca ggcccaggct cacgatcgga
     1501 tcgtgaacgc ggacgccctc cgacgcgagg gccaccagat agtatgcgta ccgcgcgccc
     1561 gcgccgtgcc gcgcgggtat caccgcatat tcagcgatag gcgcgagcgc cagaaagccg
     1621 actccgccgc tcatcaggga gtgcgcatcg actgtcggca gctcgcactc ctccggaccg
     1681 gcatatgact ggaatgcgat gggcgccgta cccagggaat ggatgcgccc gtagacatcc
     1741 gcgcaaccgg tctgctccgc tgcgacgcgc aggacctcga gcgccgcggc attggcgaag
     1801 atgatggccg ccatgcccgc gtcctcccgg ctgatccgct cgagtatcga tgcgagcatg
     1861 gtcgcgttca tctcgacgcc gccgtaatcg acgggactgt tgatcccgta gaaccccacg
     1921 gccgccgcat cgccgatcgc ctcctccatc aggccgccga agggataccg atcgcgctcc
     1981 tcccgcccct cgatgagccg cttggaacag aaatcgcgcg ccaggtcggc gaaggcctgc
     2041 cgttccgcga catacatatc attgatgatt gcgttcatga cacctccggt attctctatt
     2101 tgaagagttc cacttcggga cacgcgtgcg cgaccatgca gtagaacatg ttgagctgat
     2161 tgatctggtt ggtgctctcg tatatctgct ggagcttgac gtcgcgaaag cacttctcgg
     2221 cgccgttctc atggcggagc ccgtcggccc ccatgaggtc cagcgcgagg ttcgcgttcg
     2281 cgagccccag atcgctgcag gtgtacttgc aggccgaccc ccaccccgag gcgacgttcc
     2341 gctcctcggt ggggtagccg tcgtagtagt accatcgcag gaagcgcgtc gcgatccgaa
     2401 gatccaggag cggcgcgatg agcgagaaat accagcgcgg aagccatcgg atgacgccgt
     2461 ataccggctt cttgaacagc agcttaaaga gccccgagag ggtgagcgcg aaggcggcct
     2521 ccaggtacat gatgcgcgac aggttcacgt tgcggtacat gtccgccagg atgatctgcg
     2581 cccactggcg gtcgatgagc ggagcgccgc cgacctgctt cgtccgcgca taggccagtg
     2641 cgcgctcgta ggcgtcgcgc gcgatcccgg tggagatggc ggcggtgccg gtgcgcgttc
     2701 cgcccgtcac atggtcggtg atggtgtggc agacccagcg cgcccctttg cgcgaatccc
     2761 ggaactccgg gtcctgggtc cggatggtga ccagcgaatc gggcacaaag cactcgtcga
     2821 acagcagctc gctggccacg caggcgaact gccccatctt cttctcgtgc gcgccgaaac
     2881 tgaaaccggg cgtgccgttg gggatcgcga attgtatcca cgagtcggtc ttgctgccct
     2941 tgtcctcgat gaagttggcg atgtgccaat acgagagatg cccgttggat atgaatatct
     3001 tcttcccgtt gaggacatat ccgccctcca cccggcgcac cgacgacccg ggacgcgcgt
     3061 tatggataag aagcggctcc tgcacgtccg tgcccgactc cggctccgtc acgacgatgg
     3121 tgagcaggtg cgggtcgccg gcgcgttcgc ttttcgccac gtcggcgaac accttccgaa
     3181 tgatacgtgc attcatggag ggaaagaggg tcgccgcgcc gcagtagtgc accgcgatca
     3241 catgccccag ccccgcgcac ccggctgaga tctcctccat gaacggatac accgccagga
     3301 agctcatgcc cttcccgccg aaaatcttag gtataaaaag cgtaaacagc ccccactccg
     3361 ccgccttctt tacgaactcg agggggagat agttgtggtc ccgcatgcag cgcaggtccg
     3421 tgtcgagggc gatcggccgg atcacctcgc gggtgaattt tctggcgatg gcgatggcct
     3481 ccgcgacctc gcgatgggcc cccgccggca gccggtgctg gcactcgagc ccgttgaaca
     3541 cctccctgat cgtcggcccc tgttcgagca gcgccgcccg ctcccgtctt ccgatcttcc
     3601 atggtttcat cgtcgcgacc tcccgttccc gcccgcgccc cgcgacaagc ggcatgtgta
     3661 tgatatgtga aaacggcccg accgtttccg tccccgcctt tacgacacca atgactcggc
     3721 tatatgacgc cccatcgaca tgtcataggt cccgggcagg acgccgtcgc gctccagctc
     3781 ctttttgcga atgcgctgcg tttccgtttt gggaaactcc tcgacgatcc gcacgaagcg
     3841 cggcagcgcg aaccggggca ggtcctcgct gaggaattcg accagctccc gctcggacag
     3901 cgagcgcccc tccaccagcg agaccgacgc catgatctca tcctcggcca gctccgacgg
     3961 cacggcgtac acggccgcct cgaggactgc ggggtgccgc tggatcgcgt gctccacctc
     4021 gtaggcggac acgttctcgc ctttgatgcg catgaactcg gcgttgcggc ccacgaaata
     4081 gagatatccg tgttcgtcct tccgcaccag gtctcccgtg tagatccagc cgtcgcggag
     4141 tttttcgccg gtggccttct cgttcttgaa gtactcgacc gagcgacgtt cgcccttcgc
     4201 ggcgaagatc agctcgcccg gcatgcccac cgggacatcg tttccctcgc ggtccaccag
     4261 gcggtacacg accccggggc ccggcctgcc gatggagccc accggcgcgt tccccaggtt
     4321 catgatgctc ttgccgccgc cgtccaccgc gccgtaaccc tcgtagatcc tcacgccgaa
     4381 gcgccgctcg aattgcggcc agaggtcagc cgggcatgcg gccgagatgg tcagccgcac
     4441 acggttgtcc cggtcgtcgg gacgctccgg ctgcttcatg agaatgggga tcatcgcccc
     4501 gatcgtattg aaggccgtga cgccgttggc gcggatatcg tcccagaacc tgctcgcgga
     4561 gaattttcgc gccaggacga ccttcgcccc ggtgtgcaac ccctgcgtga tggtgagaaa
     4621 cagcgcattg ccgtggaaga gcggcaggca ggtgtacagg acatcggagg gacgatacaa
     4681 tgcataggcc gcgatggaga gcagcttcac cgtggttttt ccgtagcgat acaccacgcc
     4741 cttgggaagc ccggtggtcc ccgacgtgta caggataaag cagatatcgt ccgtgtcgta
     4801 accgccatcg ggtttcgacg cgggcaggcg cgtggcgacc gaaagagaac gggcgccctt
     4861 cggaaggggc ggctgctcgc ttcccggctc gcggttgacg atcacgaccg gttgccggat
     4921 cagcctgttc cgtattttga tgaacacgtc cagcaggtcc tcgtcgatcg ccagaaaggc
     4981 cgcttcgctg tggttcagaa tgtacagcag gctctcgccc ctgagcgagg tgttcaccgg
     5041 gaccgagtac atcccgatct tttgcgcgcc gatgaagacg tccaggtagg ccgggcaatt
     5101 ccccatcatg atggccacgc cccgcgcccg gccgccgccg agcgagagca agaggtgggc
     5161 gacccggttc gcgtttcggt ccatgtgccg gaaggaaaaa cacgttccct cgaacacgat
     5221 gcacggccgc tcgggatgca tccgggcgcg ttcctccagg agctccgccc aggacatgtc
     5281 ctcggcgatc tcccggcttc ggacgaggct cccgaggttg cgcgcgagcg tcccgcgccg
     5341 cagctcatgg gacgcaaagg ccgcaagcgc cttcgccagc ttcagtttga tgataaggtt
     5401 ttgcatacga tacatggttg tcccctctat gtccgttgat gtcacgacgc tctactgcac
     5461 cccggccgtc gccatgcccg ctccccgcga cggcgcctcg aggatgtgga tgcacatggc
     5521 cgcctcctcc tgccccagcg ccccgccgcc gttttctgcg agcgcgatgc gggcccccgc
     5581 cacctggcgg ccgccggcct cgccgcgcag ttgcgtgacc agctcgtgga tctgcgcgag
     5641 ccccgaggcg ccgatggggt gcccgcgcga ctcgagcccc ccgctggtgt tgatggggat
     5701 ccgcccgccg atgctcgtct cgccccgcag ggcgatcacg cccccctcgc ccggcgcgca
     5761 aaacccgagc gcctcggcct gatggagctc gccgtaggcc gtggcgtcgt gcacctcggc
     5821 cagatggatc tcccccgggc ccaggccgga cttctcgtag gcgatgcggc tcaagcgctc
     5881 gccgatatcg atatcgtcga aatcgcgctc gcggccggat ccgagcacgg aggcgcgcac
     5941 acgcaccgcg cgggaggcga gccccagacg ccgcaccgtg tcgccgtcgc agagaacggc
     6001 cgacgcggcg ccgtcgccga tgggagcgca catggagcgc gtgagcggcc aggagaccag
     6061 gcgatcggcc atcacctcct ccggcgtgac ccggaaggtg tactgcgcat tgggattgag
     6121 cgacgagtgc aggtggttct tcgacgcgat agcggcgagc tgttcgatgg tgctgccgta
     6181 tttccgcatg tgccagcggg ccgcgaaccc gtacacgtcc atgaacggcg aatggtcgcc
     6241 cgtgagcagc ttcctgaggt cccgcaccgt gtccgacccg agcatctccg aaagttggag
     6301 atacaccctg aacatgtcgc cgagctcgcg catcctccca taacgcgccg cgccgttttt
     6361 ccccttccgc gcctttgttt ccgcgccgcc tcgatgggcg cgcgaaccgg cgtaccggcg
     6421 cacatgttcc gccatgcgcc gtcggtcgtc atcgtccatg ccaaaacgcg acaggtccgc
     6481 gatgatgcgg gagatgttct cgatgtcgag gccgccgagg aagccggcga atatcttgta
     6541 cttgttgtgg tgatggacct tctccgcccc cacggccatc gtgatgtcgt acaggccgct
     6601 cgccacgccc agccaggcgt gatggaaggc ggtcgatccg cccgcgcagg cgttttcgat
     6661 attcgtgatg ggaatgccgt caatgcccag gggccgcagc gcgacctgcc ccctgatgga
     6721 ctcctgcccg cgcgcatagc cccacccgga attggcaaac cagagcgccg caaggtcccg
     6781 gcgcgcaatg cccgcctcgc ggaggcaggg aacgaccgtt gccgcgacga ggtccttgat
     6841 ccccatgtcg aggtacttgc cgaacttgat ggtgtgaacc gcaatcacat agacgtctct
     6901 catatgctga taccgccgca ttctgccgat gttgtccgcc cggtcgatga ccgagcgttg
     6961 acgcgaatat agcggattgc gcggaggctg tcttccacgg catcgagaag gcccgattga
     7021 actcaattat gtgcaatcat ttgcccccgc atcccgtcgg cggcagcagg ggccccccaa
     7081 atactgcttg taattatcag ctcgaccgtt cacgatatgg ccgcggaacg tgaaacgaca
     7141 gacgccggca cccatgacac cacacacacg catataccat atcctgctcg gtatgatgct
     7201 cgcccttacg ggctcgtgcg caaataccga tgcgcccgcg ccggggacca tggacttgag
     7261 cggccaatgg gagctcgtcg agacggtggc aggcggtccg gagcttcccg actatcgcgc
     7321 gaccggcgcc gtaccggtta ccatacccgg ccggctggat catatcgtgg gccgaacggc
     7381 ggagcatgcc gcgaccgtat ggcttcggaa acgggtgttc ctcggcaggg agctcgccgg
     7441 gtccatgctg gtgctcagca tcaacaacat cggcatcgcg gacgaggcgt acgtcaacgg
     7501 cgtgtgcgtc ggggcgagcg gcaccttccc ggcaggcggc ggcacccggg ggtacgatct
     7561 ggcatggcac cgcgaccggg agtatcacat ccccgcggcg ctcctgcggc ccgaccggga
     7621 gaacctgatc gccatcaagg tgtttttcca cgtgatcgac ggcgtgcggg aacgaccgtc
     7681 gctgtcgtcg gcgcgggcct tgcacgaggc gaaccgcttc agggagtttg tcccgtcggc
     7741 gaacaatctc aaccctctca ttctcgcgct cttgctgtac acgctcctca tcatctcgat
     7801 gaagggagcg gtgaaccgcc gcctggccgt gtactcgaac atcttcatca tggccgtctt
     7861 cggggtcacg gtgctcatgc tgggcctgcc gcgcttcgac gacaatctct accgattcaa
     7921 gctgtttctc cttttatacg tgctcgctga tttttccctg atcctgttca tacaggaatt
     7981 tttctccatc aggtcccgcg cgctcacggc gggcgccgtg tcggtg
//
LOCUS       DAORHF010000172         8001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8614_length_8001_cov_4.312107, whole genome shotgun sequence.
ACCESSION   DAORHF010000172 DAORHF010000000
VERSION     DAORHF010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8001
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8614_length_8001_cov_4.312107"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PKJ16_13165"
     CDS             complement(<1..1321)
                     /locus_tag="PKJ16_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004489886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV47987.1"
                     /translation="MRDFNEFNSIQIRLASPEMIREWSYGEVKKSETINYRTLKPERD
                     GLFCERIFGTTKEWECYCGKFKSIRYKGVVCDRCGVEVTHYKVRRERGGHIELATPVA
                     HIWYYRSVPSRMGLILGLTVNQLKQILYYEKYVVIDPGATDLNAGDLLDEDAYNYYYE
                     NFGDSVIIDIGAPAIKDMLTKLDLNEESRTLREKILDDQMEGRVDKKDIKRLEVIEAF
                     RDSGNKPDWMILEVVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNSRLKRLLML
                     KAPDIIVRNEKRMLQEAVDALFDNSKRKRVAVKGKGNRPLKSLSDMLKGKQGRFRQNL
                     LGKRVDYSGRSVIVIGPELKLHQCGLPKKMALELFKPFIMKRLVDRNFVQNIKSAKKM
                     VENERPEVWDVLDEVVSEHPVLLNRAPTLHRLSIQAFEPVLVEGKAIR"
     gene            complement(1365..4844)
                     /gene="rpoB"
                     /locus_tag="PKJ16_13170"
     CDS             complement(1365..4844)
                     /gene="rpoB"
                     /locus_tag="PKJ16_13170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016524629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HNV47988.1"
                     /translation="MQKGKKVVRFGKIAMGVPLPNLIEVQTKSYEWFLQRHTAPAKRK
                     DQGLQAVFKEVFPIESPHEDVVLEFLDYELGDPKYFEQECKDRDVSFAAPLKATIRLI
                     KKDSMEVREQNVYMGDIPLMTDRGTFIINGAERVVVNQLHRSPGIFFFYDEAERIYNA
                     RLIPDRGSWLEFEMDKKGYIVARIDRKKKFPLTLLVKALGFSANEEVIKLFYPTRTVE
                     IDSAEKYAGLIGNRVARDVVSTETGEVILSAGGRISIDTIDILKEENITSVELIQYPN
                     NKEDAYLINSLDKDSELIDSIADEEYSVVDKAILKIHEIMRPGEPRNLDNARAEMNRM
                     FFNPKTYSLGGVGRYKINKKFRFDEKKNIQTLDKDDIIATVKYFLFLIAEDDEYTVDD
                     IDHLGNRRVRSVGELIMNQIKVGFQRMERVIKERMTIQDLDVVTPQALISIKPITAVI
                     NEFFGSSQLSQFMDQTNPLAELTHKRRLNALGPGGLSRERAGFEVRDVHPSHYGRMCP
                     IETPEGPNIGLIVSMSSYARINEYGFLETPYRKIDSGKVQETEQYLTADEEDRHFIAQ
                     ANAEIDEAGRLTQNLLPCRNRGNFPYKRPEEIQYMDVSPSQIFSVSTSLIPFLEHDDA
                     NRALMGSNMQRQSVPLLTEEIPLVGTGIESAAAYDSGVLLIAQRAGDVIFADAKMIKV
                     QTSSGEIDSYELLKYKRTNQGTCFNQKPIVVAGQKVKKGDVLADGPSTDLGKLALGKN
                     ILVAFMPWMGYNFEDAILISERLVRDDVFTSVHIEQFEVEARETKLGREVITRDIPNL
                     NEKAFRDLDFEGIVRVGAYVKPDDIMVGKVTPKGEQDLTPEYKLLHSIFGEKAREVRD
                     TSLRVPNGVEGIVIDVKRFSREKGDELSPGVEELVKVYIATKRKISVGDKMAGRHGNK
                     GVISRILPEYDMPYLPDGTPVDIVLNPLSVPSRMNLGQLLETELGWAAHELGLICETP
                     IFDGAKETEIKEYLRKAGLPETSKIQLYDGRTGVPFENEVMVGMIYMLKLAHMVDDKI
                     HARSTGPYSLVTQQPLGGKAQFGGQRLGEMEGWAREAYGASYTLQELLTVKSDDMIGR
                     ARIYEAIVKGISTATSPGIPESFNVLIQELRGLALDVRIYDEGSKEISLAEWSEGFSK
                     PKRKIKLDTLKNI"
     gene            complement(5096..5479)
                     /gene="rplL"
                     /locus_tag="PKJ16_13175"
     CDS             complement(5096..5479)
                     /gene="rplL"
                     /locus_tag="PKJ16_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008892336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HNV47989.1"
                     /translation="MAKLSVNELLDAISELTLIEAAELVKAMEDKFGISAAAPVAIAA
                     VGAPAGGGEEAVEKTEFDVILAGFGDNKINVIKEVRAITGLGLKEAKELVEAGGKAVK
                     EKVGKEEAEKIKEQLVKAGATVELK"
     gene            complement(5630..6145)
                     /gene="rplJ"
                     /locus_tag="PKJ16_13180"
     CDS             complement(5630..6145)
                     /gene="rplJ"
                     /locus_tag="PKJ16_13180"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HNV47990.1"
                     /translation="MVKQYKVDAVSDLVQRLTAKKNIILTNYSGIPVSALSTLRRKLR
                     DKGADYKVVKNNLFIRALRDTGYTEIDSYVKGPVAVTFTGTDPSEAAKVLKEFQKEFK
                     QFSFTAGVIDNVTYREEQIRTIADLPSREAVLAQIMGLINGPARGIATGANQIMASLA
                     RAIQQVGEKNG"
     gene            complement(6145..6827)
                     /gene="rplA"
                     /locus_tag="PKJ16_13185"
                     /pseudo
     CDS             complement(6145..6827)
                     /gene="rplA"
                     /locus_tag="PKJ16_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
     gene            complement(6881..7312)
                     /gene="rplK"
                     /locus_tag="PKJ16_13190"
     CDS             complement(6881..7312)
                     /gene="rplK"
                     /locus_tag="PKJ16_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HNV47991.1"
                     /translation="MAPKKEVVTQIKLQIPGAQANPAPPVGPALGQHGLNIMEFCKVF
                     NERTKGQPGLILPVVITVYKDRTYTFIIKTPPASVLIKKTLGLEKGSGEPNKTKAGKI
                     NRAQLEEIAKTKMPDLNANDIDAAVKIIAGTARSMGVEVEE"
     gene            complement(7435..7998)
                     /gene="nusG"
                     /locus_tag="PKJ16_13195"
     CDS             complement(7435..7998)
                     /gene="nusG"
                     /locus_tag="PKJ16_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HNV47992.1"
                     /translation="MAKEWYVIHTYSGHENKVKLAIEKKAQNLRLEDKIGEIKIPTFD
                     DPYIAKNGKKKTKPRKIVPGYVLVEIDLDDDNWDNIWGIIRNTPGVTHFVGAYGKTNK
                     PRPLKKSEVNALLFDKLDERETKERAAQVINFSVGEHVKVIDGPFNNFNGVIEEIYPE
                     KGRLRVKVEIFGRGTPVELDFIQVGKI"
BASE COUNT         1376 a         2495 c         2288 g         1842 t
ORIGIN      
        1 gccggatcgc ctttccctcg accagcaccg gctcgaaggc ctgaatgctc aggcggtgca
       61 gcgtgggggc gcggttgagc agcaccgggt gttccgacac cacctcgtcg aggacgtccc
      121 acacctcggg ccgttcgttt tcgaccatct tcttggccga cttgatgttc tgcacgaaat
      181 tgcgatccac aagccgcttc atgatgaacg gcttgaagag ctccagcgcc atctttttcg
      241 gcagaccgca ctggtggagc ttgagctccg gcccaatcac gatcaccgag cggccggaat
      301 agtccacgcg cttgccgagc aggttctgac ggaagcgccc ctgtttgccc ttgagcatgt
      361 cggagagcga cttcaggggg cgattaccct tgcccttcac ggccacgcgt ttacgcttgc
      421 tgttgtcgaa gagcgcgtcc accgcctcct gcagcatgcg cttttcattg cggacgatga
      481 tgtccggcgc cttcagcatg aggaggcgct tgagccggct gttccggttg atcacgcgcc
      541 gatagaggtc attcaggtcg gaggtggcga agcggccgcc gtcgagctgc accatgggcc
      601 tcagctcggg agggattacc gggaccacct cgaggatcat ccagtccggc ttgtttccgg
      661 aatcgcggaa ggcctcgatc acctcgagcc gcttgatgtc cttcttgtcg acgcgcccct
      721 ccatctgatc gtcgaggatc ttctcgcgca gggtgcgcga ctcctcgttg agatcgagct
      781 tggtgagcat gtccttgatc gccggcgcgc cgatgtcgat gatcacggaa tcgccgaaat
      841 tctcgtagta gtagttatag gcgtcctcgt ccagcaggtc gccggcgttg aggtcggtcg
      901 cgcccggatc gatgaccacg tatttttcat aatatagaat ctgcttgagc tgattcacgg
      961 tgagccccag gatgaggccc atgcgcgaag gaaccgaacg ataataccag atatgcgcca
     1021 cgggcgtcgc gagctcgatg tgcccgccgc gctcgcgacg caccttgtag tgggtcacct
     1081 cgacgccgca gcggtcgcag accacgccct tgtagcggat cgacttgaac ttaccgcagt
     1141 agcactccca ttccttcgtg gtcccgaaga tccgctcgca gaacaaaccg tcgcgctcgg
     1201 gcttgagcgt gcggtagttg atcgtctcgg acttcttcac ctcaccgtag gaccattccc
     1261 tgatcatctc cggcgacgcc agcctgattt gaatggaatt gaactcgttg aaatctctca
     1321 tgggttaccc tttcgtttga aatgcgggaa cgccgtcccc gctctcagat gttcttcagc
     1381 gtatcgagct tgattttccg tttcggcttg ctgaatccct cgctccattc cgcaaggctg
     1441 atctccttgc tgccctcgtc gtaaattcgc acgtcgagcg cgagcccgcg cagttcctgg
     1501 atgagcacgt tgaacgattc cggaatgccg ggactcgtcg cggtggagat gcccttcacg
     1561 atcgcctcgt agatgcgcgc gcgaccgatc atgtcatcag acttcacggt gagcagctcc
     1621 tgcagggtgt aggatgcgcc gtaggcctca cgcgcccatc cctccatctc cccgagccgc
     1681 tgtccgccga actgcgcctt gccgcccagc ggctgctgcg tcaccagcga gtacgggccg
     1741 gtggagcgcg catggatctt gtcgtccacc atgtgggcga gcttcagcat gtagatcatg
     1801 cccaccatca cctcgttctc gaagggaacg ccggtccggc cgtcgtagag ctggatcttc
     1861 gaggtttcgg ggagcccggc cttgcggaga tactccttga tctcggtctc ctttgcgccg
     1921 tcgaagatcg gcgtttcgca gatcaggccg agctcatgcg cggcccagcc gagctccgtc
     1981 tccaggagct gccccaggtt catccgcgac ggcaccgaaa ggggattgag cacgatgtcc
     2041 accggcgtcc cgtcggggag atacggcatg tcgtactcgg gcaatatgcg ggagatcacg
     2101 cccttgttgc cgtgacggcc cgccatcttg tcgcccacgg agatcttgcg cttggtggcg
     2161 atgtagactt tcaccagctc ctcgacgccg ggggagagct cgtcgccctt ctcgcgcgag
     2221 aagcgcttca cgtcgatcac gatgccctcg accccgttgg gaacacgcag cgaggtatcg
     2281 cgcacctcgc gcgccttttc gccgaagatc gagtgcaaca gtttgtattc aggggtgagg
     2341 tcctgctcgc ccttgggcgt gaccttgccc accatgatat catccggttt cacataggcg
     2401 cccacgcgca cgatcccctc gaagtcgaga tcgcggaacg ccttctcgtt caggttcggg
     2461 atgtcgcggg tgatcacctc gcggcccagc ttggtctccc gcgcctccac ctcgaactgc
     2521 tcgatgtgca ccgaggtgaa tacgtcgtcc cgcaccaggc gctccgagat gaggatcgcg
     2581 tcttcgaagt tgtatcccat ccagggcatg aaagcaacaa ggatgttctt gcccagcgcg
     2641 agcttgccca ggtccgtcga gggaccgtcg gccaggacat cccccttctt gaccttctgc
     2701 ccggccacga cgatcggttt ctggttgaag caggtcccct ggttggtccg cttgtacttc
     2761 aggagttcgt agctgtcgat ctcgccgctc gaggtctgca ccttgatcat cttcgcgtcg
     2821 gcgaagatga cgtcccccgc gcgctgggcg atcagaagca cgccggaatc ataggccgcg
     2881 gcggactcga tgccggtgcc caccaacggg atctcctcgg tcagaagcgg caccgactgg
     2941 cgctgcatgt tcgatcccat gagcgcgcgg ttcgcgtcat catgctccag gaacggaatg
     3001 agcgacgtgg acacggaaaa gatctgcgac ggcgacacgt ccatgtactg gatttcctcc
     3061 ggccgcttgt aggggaagtt gccccggttc cggcagggga gcaggttctg cgtgaggcgt
     3121 cccgcttcgt cgatctcggc attggcctgg gcgatgaagt ggcgatcctc ctcgtcggcg
     3181 gtcaaatact gctcggtctc ctgcaccttt ccgctgtcga tcttccggta gggcgtctcg
     3241 agaaaaccgt attcgttgat acgcgcatac gagctcatcg atacgatgag gccgatgttc
     3301 ggtccttccg gcgtctcgat gggacacatc cgcccgtaat gcgaggggtg cacgtcgcgc
     3361 acctcgaagc cagcgcgctc ccgcgagagg ccgccgggcc ccagggcgtt cagtcgccgc
     3421 ttgtgcgtca attccgcaag cgggttcgtc tggtccatga actgcgagag ctggctggag
     3481 ccgaagaact cgttgatgac cgccgtgatc ggcttgatgc tgatgagcgc ctgcggcgtg
     3541 accacgtcga ggtcctggat ggtcatgcgc tccttgatca cgcgctccat ccgctggaac
     3601 cccaccttga tctggttcat gatcagctcg cccaccgagc ggacgcgccg gttccccagg
     3661 tggtcgatat cgtccaccgt gtactcgtcg tcctcggcga tgaggaacag gaagtacttc
     3721 accgtcgcga tgatgtcgtc cttgtccagc gtctggatgt tcttcttctc gtcgaagcgg
     3781 aacttcttgt tgattttgta tcggcccacc ccgcccaggc tgtaggtctt gggattgaag
     3841 aacatgcggt tcatctcggc gcgggcattg tccaggttgc gcggctcgcc cggccgcatg
     3901 atctcgtgga tcttcagaat ggccttgtcc acgacgctgt actcttcgtc ggcgatcgag
     3961 tcgatgagct ccgaatcctt gtccagcgag ttgatcaggt acgcgtcttc cttgttgttc
     4021 gggtactgga taagctcaac cgacgtgatg ttctcttctt taaggatgtc gatggtgtcg
     4081 atgctgatgc ggccccccgc cgagaggatc acttcgccgg tttccgtgct caccacgtcg
     4141 cgcgccaccc ggttgccgat gagccccgcg tacttctcgg cgctgtcgat ctcgaccgtc
     4201 cgggtcgggt agaagagctt gatgacctcc tcgttggcgc tgaagcccag cgccttcacc
     4261 agcagggtga gcgggaactt tttcttgcgg tcgatgcgcg ccacgatgta gcccttcttg
     4321 tccatctcga actcgagcca cgaaccgcgg tcggggatca ggcgcgcatt atagatgcgc
     4381 tccgcttcgt cgtaaaagaa gaaaatgccg ggcgaacggt gcagctggtt caccaccacg
     4441 cgctccgcgc cgtttatgat aaaggtgccg cgatcggtca tgagcggaat gtcgcccatg
     4501 tacacgttct gctcgcggac ctccatgctg tcctttttaa tgaggcggat cgtcgccttc
     4561 aggggcgccg cgaacgagac gtcgcggtcc ttgcactcct gctcgaaata tttcggatcg
     4621 ccgagctcgt aatcgaggaa ctcgaggacc acgtcctcgt gcggactctc gatggggaag
     4681 acctccttga agacggcctg gagcccctgg tccttgcgct tcgcgggcgc cgtatggcgc
     4741 tgcaggaacc actcgtacga tttcgtctgg acttcgatga ggttcgggag cggaaccccc
     4801 atggcgatct tcccgaacct gacgaccttt tttccctttt gcatggacag cctatccctc
     4861 gagcatgagt atcagaagcc cgccggctcg cacgttgcgc gcaatcggcg tcacacactt
     4921 acatgcggca cgcgtgagtg gcaaaaatgg cttgtgcgtt gcggaatact gcacggcatc
     4981 aatccccatc ggggcgcatt gcaatcaaac atcgataggt ttggccgcca cgcaaccaaa
     5041 cctatcgagc acgaacgacg ctggaccgcc gtgatgcgaa cgaatgaacg gccgactact
     5101 tgagctcgac cgtcgcgccg gccttgacca gctgttcctt gatcttttcg gcctcttcct
     5161 tgccgacctt ctccttgacg gcctttccgc cggcctcaac cagctccttg gcctccttga
     5221 ggcccaggcc ggtgatggcg cggacttcct tgatgacgtt gatcttgttg tcgccaaacc
     5281 cggcgaggat gacatcgaac tcggtcttct cgaccgcctc ttcgccgccg ccagccgggg
     5341 cgccaaccgc cgcaatcgca accggcgccg ccgcggaaat gccgaacttg tcttccatcg
     5401 ccttgacgag ctctgccgct tcgatcaagg tgagctcgct gatcgcatcg agcaattcat
     5461 tcactgataa cttcgccatt acaatgactc cttatattta atgaatctat tcaagttaat
     5521 acggtctgtg ccgtaaaaac ctataagggg ccaggcatac agtcgttctc ttcgcaaaca
     5581 ccgatcaaac aacctaccta aaaccctctt gtgtgcgtat tcgtcgcccc tagccgttct
     5641 tctccccgac ctgttggatc gcgcgcgcaa gggacgccat aatctgattg gcgcccgtcg
     5701 cgataccgcg cgccggcccg ttgatgaggc ccatgatctg cgcgaggacc gcctcgcgcg
     5761 acggcaggtc ggcgatcgtg cggatctgct cctcgcgata ggtgacatta tctatcacgc
     5821 ccgccgtgaa gctgaactgc ttgaactcct tctggaactc cttgagcacc ttcgcggcct
     5881 ctgaggggtc cgtgccggta aaagtgaccg cgaccggccc cttcacgtac gaatcgatct
     5941 ccgtatagcc ggtgtcgcgg agcgccctga tgaacaggtt gtttttcacc accttgtagt
     6001 cggcgccctt gtcgcggagc ttgcgacgca gcgtactgag cgcggatacg gggatcccgc
     6061 tgtaattggt cagaataatg ttcttcttgg cggtgagccg ctgaacgagg tcgctcacgg
     6121 cgtcgacttt atattgttta accattatcg ttcaatcccc ttgtagttga ttttgattcc
     6181 ggggcccatg gtcgagctga tatgcaccga cttgatatag ttgcctttcg catccgacgg
     6241 cttgtcgcgc acgatctggt tgaagaaggt cttgatgttc tctgtgagct gcgacgcgtc
     6301 gaaatcggtt tacccacgct cacgtgcacc acgccggtct tgtcgcactt gtactcgatc
     6361 cggccgccct tgagctcctt cacgataccg gcgacatcgt cggtgacggt cccggttgcg
     6421 ggcttgggca tgaggccctt gcggccgagg acggggccga gcttgcccac ctccttcatc
     6481 atgtccggcg tggcgaccac ggcgtcgaag tcgatccagc cgcccttgat tttatcgatg
     6541 acatcctccg cgcccacgtg atcggcgccg gccgcggtgg cctccttctg cttgtcgccc
     6601 ttgcagatca ccagcacctt cttttctttc ccggtgccgg cgaggagcgt taccgcgcca
     6661 cggatgtttt ggaagctctt gtggacgatc ttgacgtgaa cctcgacggt ctcgttaaac
     6721 ttggagaatt taacttcctt caagagcttc acggcgtcct cgagcgcgta gagcacggtc
     6781 ttgtcgacct tctcgcgcgc gactgcgatc ttcttgccgc gtttcataaa gccttacctc
     6841 gttccttgag tggtgtacgt cagttcgaat aatcgtgcga ttattcttcc acttccacgc
     6901 ccatcgagcg cgccgtcccg gcgatgatct tgacggctgc gtcaatatca ttcgcattga
     6961 gatcgggcat tttcgtcttg gcgatctcct cgagctgcgc ccgattgatc ttgcccgcct
     7021 tcgtcttgtt gggctcgccg gagcccttct cgagacccag cgtcttcttg atgagcaccg
     7081 acgcgggcgg cgttttgatg atgaacgtat aggtgcgatc cttatatacc gtaatgacga
     7141 ccgggagaat gagccccggc tgccctttgg tccgttcgtt gaagaccttg cagaactcca
     7201 tgatgttgag cccgtgctgg ccgagcgcgg gaccgacggg cggcgcggga tttgcctgcg
     7261 cgccggggat ctgcaatttt atctgcgtga cgacttcttt tttcggtgcc attgatgata
     7321 tcctgctttt ctgtaacagc gaggcattgc ctcgccccta cgatctcatg aaatggtaca
     7381 gacaaggcag tgcctcgtct ctacggacaa aaaattaaat cccgatacgg cggatcaaat
     7441 cttccccacc tggatgaaat ccaattccac cggcgtgccg cggccgaaga tctcgacctt
     7501 tacgcgcaat cggcccttct ccggatagat ctcctcgatg acgccgttga aattgttgaa
     7561 cgggccgtcg atgaccttca cgtgttcgcc cacggagaaa ttgatcacct gggcggcgcg
     7621 ctccttggtt tcacgctcat cgagcttgtc gaacaggagc gcgttcacct cgctcttctt
     7681 gagagggcgc ggcttgttcg tcttgccgta cgcgcccacg aaatgcgtca cgccgggggt
     7741 gttgcggata atcccccaga tattgtccca gttgtcgtcg tcaagatcga tctccaccag
     7801 cacgtagccg gggacgatct tgcgcggctt ggtcttcttc tttccgttct tcgcgatata
     7861 cggatcgtcg aaggtcggga tcttgatctc gccgatcttg tcctcgaggc gaaggttttg
     7921 cgccttcttc tcgatcgcca gcttcacctt gttctcgtgc cccgagtagg tatggatgac
     7981 gtaccactcc ttcgccatgg c
//
LOCUS       DAORHF010000173         7926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8746_length_7926_cov_4.916021, whole genome shotgun sequence.
ACCESSION   DAORHF010000173 DAORHF010000000
VERSION     DAORHF010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8746_length_7926_cov_4.916021"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="PKJ16_13200"
     CDS             <1..2033
                     /locus_tag="PKJ16_13200"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV47993.1"
                     /translation="TGHDTEILIEACIGIGEALVSGEVTPDQYFFDWFSGTETKRVIG
                     DKRIAIYAIDDPPFVRNVDNPEGQRTAAVLSPDEVRTLATIAVDIQARYGFPVDIEWA
                     QCNGEFFIVQSRPITTINYAGIEGEWTTADFKDGGVSSTVCSQFMWSLYDLVWETAMP
                     SYLIQAKLYRNDPGITWGDMFYGRPYWNAGAVKAGVMCIPGFNERKFDEDLGIQVAYE
                     GDGHVTQITPKSILAGLRVLNALNTEFKKQMDLAPQFKERQLAKLAELDNIDPSAMPR
                     DDFYAFYERFIYEEYFLSESTYFTLIFYNSNLNSIFKESFEAVADRVNLLNLISGLTD
                     LSHMIPVYRMWDLKKRIAADASAYDYWKRTSVADLIADWRAGTTDHCMDAVREYIDEV
                     RFHSTRELDITVPRYGEDPAPVMESVKNSLDLDERHDPRELTRKQHDAYLAEREKYLA
                     AHPFWKRRKADRMLSLLRKFLWWREELRDLSTKYYHQVRRFTLVMAGHLASAGAIEHP
                     DDIFFLPVRDVVDIAKGVITPERARALVRRNRRYYESFRNYANPNEIGSRYAQKRRAI
                     PEGQRVLNGIASSPGQVTGRVKVIRDIFDADRLEKGDILITRFTDPGWTPKFSLLAGV
                     ATETGGLLSHAAVISREYGIPAVLAVDNITAILHDGQEVTIDGDAGMVHLLN"
     gene            2095..2388
                     /locus_tag="PKJ16_13205"
     CDS             2095..2388
                     /locus_tag="PKJ16_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47994.1"
                     /translation="MNILKKAYPAALIITMVIFAFSLLPEMYVTWYLFAPAILLGVLI
                     VIVAWDRALRSRWLGIVILVVALVGIQPILDTVFFAAGIACCALALVRYKKLK"
     gene            2423..4051
                     /locus_tag="PKJ16_13210"
     CDS             2423..4051
                     /locus_tag="PKJ16_13210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HNV47995.1"
                     /translation="MKQIRTALIAIIVATLFAAAGCSQSRPGAYTIKSSGSFVLPLYQ
                     GTKVNAIMFTPVTDNPNETFPSIIMISSWSMSEYEYYVQGKRFAEEGYNVLMYDPRGW
                     LDGELEIASDKDVNDLKTAIDWLLKNAKVKMGADGKPVLAAGGISYGSVICGLTASQD
                     DRIKVLFALSSMGDARRAMFDQDTAHQLMMWLLVKTGEWKGRLSKEVKDKMDDAFNNR
                     NIEEQRAWLSKRSPMNYLETFNKKNIPVYLSINMFDELFWGNSIVDFFSRLTVPKRLD
                     MNLGDHAQSESLGLLGFYEGSQPWNATLEWLDYWLKGKDNGVLKKPKVTMVVARSKVR
                     EEFENWPSARVSTQSLYLAPRPRESFAYGELVEKPIDSPQVPFNTINSGKISGISAGL
                     PLVSYPLMGHTPIQVRTWLPGVRDSRSIVYTTPSLARPMKIRGVPRLNVWIEPSRPQA
                     QIIAHLFDVNSAGIATLITHGPATLHSAKPNEPVQLSLELCIISYDVPIGHRLALAID
                     TYDPLYNPPTSEPYRIKFVYGKNHQSNIVVPIMK"
     gene            4065..4736
                     /locus_tag="PKJ16_13215"
     CDS             4065..4736
                     /locus_tag="PKJ16_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV47996.1"
                     /translation="MIALKNVKKTYTLGKTEVHALRGVSLEIRKGDFVCVMGPSGSGK
                     STLLNIVGCLDRPNDGEVVINGTNVISLDRNSLADLRASAIGFIFQSFNLIPVLNVYE
                     NIEFPLYIKKEKISEADKKDRIMRLIGEVGLTEYVNHKPDELSGGQRQRVAIARALVT
                     KPQIVLADEPTANLDSETARQIVRLMRQLNDEEQVTFVFSTHDQSITEYATSIHRLKD
                     GALAN"
     gene            4747..6003
                     /locus_tag="PKJ16_13220"
     CDS             4747..6003
                     /locus_tag="PKJ16_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV47997.1"
                     /translation="MQWVQHILKLSLRNIRRHPVKNVLTGVMILLSSLAFFFSASLSK
                     NSELGWRGFLGKTFLGHYHVTAYAGLDRDYSFSSYYLPDAYIPDELTRYLDENKVEYA
                     RRVKLGAVVYNRDKGEFENTLCTVIGMDMDAELKKLTNMDIVQGKYDPSNPNGALIWK
                     EYADFLKLKVGDEITVFLRDVDNGNYPYTFTVTGIYFQKEGSNIQDKAYLRIFPLVLV
                     PYRQLTTLLGVEPDRATEIAIWDFDERHSSAVRSIAKKHKMEFMYGEEIFAIIFGTVD
                     FLHFLGGFLGVIILTILVVSTLNINMISFFERQKEIGTIIAIGGRARWVVTLLVSELV
                     TFSTVAVIISIVIYLGLSAAMSMGVDFGESSVLFAGNRFYMGLVGKSVLPTYVTIVVT
                     MLVSALYPIYLSTRINPVDTFREGEL"
     gene            6000..7163
                     /locus_tag="PKJ16_13225"
     CDS             6000..7163
                     /locus_tag="PKJ16_13225"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV47998.1"
                     /translation="MIRLKLAYRNAIKHRWSSIVLMSVFTIAVLSTFWMFGFSNYLDG
                     VLVETFRDSYGDMVFFVNYAKRGDLARILKDVPAAKIVYEREVNAIFDNPKKSSIIRV
                     IEITGQNRGAIGKYLRPAVGTVPTRPDEALLSEIYAESEFKVGDTFHITTTTPDKIIN
                     TLKYTVSGINRADPAKTVLNGFIVTEESMNLLVNSSEHVNMVYVYLGDRLGERAAVKA
                     TFDRITELFAKNGIGVKDSWTVYHPLDKLEIFNVILGAGRMIVFIVILPLVGAVVGAI
                     VWMYSWRRRKEIWTFVSLGMKDREVLSVMIMEYVLIAAAGVVLGIGLGYVTSFLSDAG
                     NVLLKFSITFRMPLIAKIGVGDLALIPVVLFLCVIVCSMPPIRRIIKSTPFSY"
     gene            7278..>7926
                     /locus_tag="PKJ16_13230"
     CDS             7278..>7926
                     /locus_tag="PKJ16_13230"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV47999.1"
                     /translation="MMNITKTVALFAICALGLPAHGMSGDEILKISDSRFIPRICSYI
                     ITIKTEDQSKKTSVNVAKGYKKGNDRNVIIVREPKRIAGSVHLRRGNVVWSYYTTNKR
                     LMKMAYHSTFMGTMVNYGDIMATELSYDYNVKETKVNPDTYVLTLVPKPGHEGYAKIV
                     LTIDRKTMLPKKREYYALSGILMKMSDVKEIRTQNNVTAYLVQDFYEPLKERHSLV"
BASE COUNT         1663 a         2396 c         2318 g         1549 t
ORIGIN      
        1 tcacgggaca cgacaccgag atcctcatcg aggcctgcat cggcatcggc gaggcgctgg
       61 tgagcggcga ggtgacgccc gatcagtact tcttcgactg gttttctgga accgaaacca
      121 agcgcgtgat tggcgacaag cgcatcgcca tctatgcgat cgacgatccg cccttcgtcc
      181 ggaatgtcga caatcccgaa gggcaacgca ccgcagcggt cctttccccg gacgaggtgc
      241 gcacgctggc cacgatagcc gtcgatatcc aggcgcgcta cggctttccc gtcgacatcg
      301 agtgggcgca gtgcaacggc gagtttttca tcgtccagag ccgtcccatc acgaccatca
      361 actatgcggg gatcgagggc gaatggacca ccgccgattt caaggacggc ggcgtatcgt
      421 ccacggtatg cagccagttc atgtggtcgc tctacgatct cgtctgggag acggcgatgc
      481 cgtcctatct catccaggcg aagctctacc ggaacgatcc gggtatcacg tggggcgaca
      541 tgttttacgg acgtccctac tggaacgccg gcgcggtcaa ggcgggagtg atgtgcatcc
      601 ccggcttcaa cgaacgcaag ttcgacgagg acctggggat ccaggtcgcg tatgaaggcg
      661 acgggcatgt cactcagata acgcccaagt ccattctcgc cgggctgcgc gtcctgaacg
      721 cattgaacac ggaattcaaa aagcagatgg acctggcgcc ccaattcaag gagcgccagc
      781 tcgcaaagct tgccgaactc gacaacatcg atccctcggc gatgccgcgc gacgactttt
      841 acgcattcta cgagcgcttc atttatgagg aatacttcct cagtgaatcg acctatttca
      901 cgctcatctt ctataactcg aacctcaact cgattttcaa ggagtccttt gaggcggtcg
      961 ccgaccgggt caatctcctc aatctgatat ccggactcac ggacctgtcg cacatgatcc
     1021 cggtgtaccg gatgtgggac ctcaagaaac gtatcgccgc ggatgcgtcg gcctacgact
     1081 actggaagcg cacatccgtt gcggacctta tcgccgattg gcgcgccggc acaacggatc
     1141 attgcatgga tgcggtacgc gaatacatcg acgaggtccg cttccattcc acccgtgagc
     1201 tcgatattac ggtgccgcgc tacggcgagg accccgcgcc cgtgatggaa agcgtgaaga
     1261 acagcctcga tctcgacgag cggcacgatc cgcgcgagct gaccaggaag cagcacgacg
     1321 cctatctcgc cgagcgcgag aaatatcttg ccgcgcatcc gttctggaaa cgccggaagg
     1381 ccgaccggat gctttcgctc ctgcggaaat ttctctggtg gcgggaggag cttcgcgacc
     1441 tctcgaccaa gtactaccat caggttcggc gcttcacgct ggtcatggcc gggcacctgg
     1501 cgtccgccgg ggccatcgag catccggacg atatcttctt tctgcccgtt cgcgacgtgg
     1561 tcgatatcgc gaagggcgtt atcaccccgg agcgcgcgcg ggcgctggtg aggcgaaaca
     1621 ggcggtatta cgaatcgttc agaaattacg cgaacccgaa cgagatcggg agccgctatg
     1681 cgcaaaagcg acgcgccata ccggagggac agcgcgtatt aaacgggatc gcgagcagtc
     1741 cggggcaggt cacgggtcgg gtcaaggtga tccgcgatat cttcgacgcc gaccgcctcg
     1801 agaagggcga tatcctcatc acgcgcttta ccgatcccgg ctggacgccc aaattcagtc
     1861 tgctcgcggg cgtggccacg gagacaggcg ggctgttgtc gcatgcggcg gtgatctcgc
     1921 gtgaatacgg catccccgcg gtgcttgcgg tggacaacat caccgcgatt ttgcacgatg
     1981 gacaggaagt caccatcgac ggcgatgccg gaatggttca tctgttaaac tgaccggccc
     2041 ggggctgtgc gccatcggcg gttgtcgtga agcaatttta ttcgaggagc agatatgaat
     2101 atactcaaga aggcgtatcc ggccgcactc atcatcacca tggtcatttt cgcgtttagc
     2161 ctgctgccgg aaatgtacgt gacctggtat ctcttcgcgc cggccatcct gctgggcgtg
     2221 ctcatcgtca ttgtcgcctg ggaccgtgcc cttcggtcgc gctggctggg catcgtgatc
     2281 ctcgtcgtgg cgctcgtggg gatccagcca attctcgata cggtgttttt cgccgccggg
     2341 atcgcatgct gcgcgcttgc gctggtgcgc tacaaaaaac tgaaataatc atgccgattg
     2401 cacacaatcg gaaggagacg ccatgaagca aatccgcact gcacttatcg ccatcattgt
     2461 cgcaacgctt ttcgcggccg ccggatgcag ccaatcgcgc ccgggcgcct acacgataaa
     2521 aagctccggg agcttcgtgc ttccgctgta ccaggggacc aaggtgaacg ccatcatgtt
     2581 cacgcccgtc accgacaatc ccaacgagac cttcccatcc atcatcatga tatccagttg
     2641 gagcatgtcg gagtacgagt attatgtcca gggcaagcgc ttcgccgagg agggctacaa
     2701 cgtcctcatg tacgatccgc gcggctggct cgacggcgag ctggagatcg ccagcgacaa
     2761 ggacgtgaac gatctcaaga ccgcgatcga ctggttgctc aagaacgcga aggtgaagat
     2821 gggtgcggac gggaagcccg tcctcgccgc gggcggcatc tcttatggtt cggtcatctg
     2881 cgggctcacg gcctctcagg acgaccgcat caaggtgctc ttcgcgctgt cgagcatggg
     2941 cgacgcccgg cgcgcgatgt tcgatcagga cacggcccat cagctcatga tgtggctcct
     3001 ggtgaagacc ggtgaatgga aggggcgcct ctcgaaggaa gtgaaagaca agatggacga
     3061 cgccttcaat aaccgaaaca tcgaggagca gcgcgcatgg ctctccaagc gttctcccat
     3121 gaactatctc gagaccttca acaagaagaa catcccggtc tacctcagca tcaacatgtt
     3181 cgacgagctg ttctggggaa actccatcgt cgatttcttt tcacgactca ccgttcccaa
     3241 gcggttggac atgaacctcg gcgaccatgc ccagagcgag tccctggggc tcctgggctt
     3301 ctacgagggc agccagccct ggaacgcgac cctcgaatgg ctcgactact ggctcaaggg
     3361 caaggacaac ggtgtgctga aaaagccaaa ggtgaccatg gtggtggccc gcagcaaggt
     3421 tcgtgaggag ttcgagaact ggccgagcgc gcgcgtttcg acccagagcc tgtatcttgc
     3481 gccgcgcccg cgtgagagtt tcgcgtacgg agagcttgtc gagaagccga tcgattcgcc
     3541 gcaggttccc ttcaacacca tcaactcggg gaagatatcc ggcatttcag ccggactgcc
     3601 gctggtgagc tatccgctga tgggccacac gccaattcag gtgcggacct ggctgccggg
     3661 cgtccgcgac agccgctcca tcgtctatac cacgccatcg cttgcacgtc ccatgaagat
     3721 ccgcggtgtc ccgcggctca atgtctggat cgagccgtcg cgcccgcagg cgcagatcat
     3781 cgcgcacctg ttcgacgtga acagcgcggg gatcgccaca ctcatcacgc atggcccggc
     3841 gacgctccat tccgcgaagc ccaacgaacc ggtgcagctc tcgctggagc tgtgcatcat
     3901 ctcgtacgac gtgccgattg ggcaccggct tgcgctcgcg atcgacacct acgatccgct
     3961 gtacaatccg cccaccagcg agccgtaccg cataaagttc gtgtacggga agaatcatca
     4021 atcgaacatt gtcgttccca tcatgaagta aggggctggc aggcatgatt gcgctgaaaa
     4081 acgtcaagaa aacctacacc ctcggcaaga ccgaggtgca cgcgcttcgc ggtgtttcgc
     4141 tggaaatccg gaagggcgat ttcgtctgtg tgatgggtcc ctccggcagc gggaagagca
     4201 ccttgctgaa catcgtcggc tgtctcgacc gtcccaacga cggcgaggtc gtcatcaacg
     4261 gcaccaatgt gatttcactc gaccggaaca gcctcgccga cctgcgggcg agcgccatcg
     4321 gattcatctt ccagtcgttc aatctgattc cggtccttaa cgtgtacgag aacattgagt
     4381 tcccgctgta cattaagaag gaaaaaattt cggaagcgga caaaaaggac cggatcatgc
     4441 ggcttatcgg ggaggttggc ctcacggagt atgtgaacca caaacccgat gagctctcgg
     4501 gcggacagcg gcagcgggtc gcgatcgccc gtgcgctggt gaccaagccg cagatcgtgc
     4561 tggccgacga accgaccgcc aatctcgatt cggagaccgc gcgccagatc gtccgcctca
     4621 tgcgccagct caacgacgag gagcaggtca ccttcgtctt ttcgacgcat gaccagtcga
     4681 tcaccgaata cgccacgagc atccaccgcc tgaaagacgg cgcgctggcg aactgagggt
     4741 gacgcgatgc aatgggttca gcacatcctg aagctctccc tgcggaacat ccgccgtcac
     4801 ccggtaaaga acgtcctgac gggagtgatg attttactct ccagcctcgc gttcttcttt
     4861 tcggcctcgc tctcgaagaa ctccgagctc ggttggcgcg gctttctggg caagaccttc
     4921 ctcgggcact accacgtcac ggcctatgcg ggactcgatc gagactacag cttttcgtcg
     4981 tattatcttc ccgacgcata tatcccggac gagcttacgc ggtatttgga cgagaacaag
     5041 gtcgagtacg cgcgccgcgt gaagctcggc gcagtggtgt acaaccgcga caagggcgag
     5101 ttcgagaaca ccctctgcac ggtgatcggg atggacatgg acgccgagct gaaaaagctc
     5161 acgaacatgg acatcgtcca ggggaagtac gatccgtcga acccgaacgg tgcgctcatc
     5221 tggaaggaat acgcggattt tctcaaactc aaggtcggcg acgagatcac cgtcttcctg
     5281 cgcgacgtcg ataacggcaa ttacccgtac accttcaccg tgaccggcat ctatttccaa
     5341 aaagagggat cgaacatcca ggacaaggcc tacctgcgca tcttcccgct cgtgttggtg
     5401 ccgtaccggc aactcacgac ccttctgggt gtcgagccgg accgcgcgac cgaaatcgcc
     5461 atctgggact tcgacgagcg gcattcctcc gccgtgcgat caatcgcgaa aaagcacaag
     5521 atggagttca tgtacggcga ggagatcttc gcgatcatct tcgggacggt cgactttctg
     5581 cattttctgg gcggatttct tggcgtgatc atcctcacca ttttggtggt atccaccctg
     5641 aacatcaaca tgatcagctt cttcgagcgg cagaaggaga tcggcaccat catcgccatc
     5701 ggcgggcgcg cgcgctgggt ggtcacgctc ctggtctccg agctcgtcac cttttcgacc
     5761 gtcgcggtga tcatttcaat cgtgatctac ctcggcctct cggctgccat gtcgatgggc
     5821 gtcgatttcg gcgagtcgag cgtcttgttc gcgggcaacc gcttctacat ggggctggtc
     5881 gggaagtcgg tgctgcccac ctatgtcacc atcgtcgtca ccatgctggt ctcggcgctc
     5941 tacccgatct atctttccac gcgcatcaat ccggtggaca ccttcaggga gggcgagcta
     6001 tgatacgcct caaactggcc taccgcaacg cgatcaagca ccggtggtcc tccatcgtgc
     6061 tcatgtccgt cttcaccatc gccgtgctgt ccaccttctg gatgttcggc ttcagcaact
     6121 atctcgacgg cgttctcgtg gagaccttcc gcgacagcta cggcgacatg gtgttcttcg
     6181 tcaattacgc gaagcgcggc gatctcgcgc gcatcctgaa ggacgtgccc gcggcgaaga
     6241 tcgtctacga gcgcgaggtg aacgcgatct tcgacaatcc gaagaagagc agcatcatcc
     6301 gcgtgatcga gataacgggg caaaaccgcg gcgcgatcgg gaagtacctg cgaccggcgg
     6361 tcggaacggt gcccacgcgg cccgacgagg cgctcctctc cgagatctac gccgagagcg
     6421 agttcaaggt gggcgacacc ttccacatca ccacgaccac gccggacaag atcatcaata
     6481 cgctcaagta caccgtgagc ggcatcaacc gcgccgaccc ggccaaaaca gtgctcaacg
     6541 gcttcatcgt gaccgaggag agcatgaacc tgctggtcaa ttcgtcagag cacgtgaaca
     6601 tggtgtatgt gtacctcggc gaccggctcg gcgagcgcgc cgcggtcaag gcgaccttcg
     6661 accgcatcac cgagcttttc gcgaaaaacg gcatcggcgt gaaggactcc tggacggtgt
     6721 atcatccgct cgacaagctg gagatattca acgtgatcct gggcgcgggc cggatgatcg
     6781 tattcatcgt gattctcccg ctggtgggag cggtcgtcgg ggccatcgtg tggatgtact
     6841 cgtggcggcg ccgtaaggag atctggacct tcgtctcgct ggggatgaag gaccgcgaag
     6901 tgctttcggt catgatcatg gagtacgtgc tcatcgcggc cgcgggcgtg gtgctgggga
     6961 tcgggctggg ctacgtcacg tcgtttctct ccgacgcggg caatgtgctg ctcaagttct
     7021 ccataacctt tcgcatgccg ctcatcgcga agatcggcgt gggggacctt gcgctcattc
     7081 cggtggtgct cttcctgtgc gtgatcgttt gcagcatgcc gccgatcagg cgcatcataa
     7141 aatcgacgcc gttcagctac tagcgaaccg cgcgcggtcg catgggcgtc ggcgagttcg
     7201 ccggtcccgt gcggaatgtt tctcttgatt aaccattcga tgcgggccgc acgcccgaaa
     7261 aacacgaggt atacggtatg atgaacatca cgaagaccgt cgcgcttttc gccatctgcg
     7321 ccctggggct gccggcgcat ggcatgtcag gggacgagat cctcaagata tccgacagcc
     7381 gcttcatccc gcggatctgt tcgtacatca tcacgatcaa gaccgaggac cagagcaaga
     7441 agacgagcgt gaacgtggcg aagggctaca agaaggggaa cgaccgcaac gtgatcatcg
     7501 tgcgcgagcc gaagcggatc gccgggtcgg tccacctgcg ccgggggaac gtggtgtgga
     7561 gctactacac caccaacaag cgcctcatga agatggccta tcactcgacc ttcatgggga
     7621 cgatggtcaa ctatggcgac atcatggcca ccgagctgtc ctacgattac aacgtgaaag
     7681 agacgaaagt gaatcccgac acctatgtgc tcacgctggt cccgaagccg ggccacgagg
     7741 gttatgcgaa gatcgtgctc accatcgacc gtaaaacgat gctgccaaag aagcgggaat
     7801 attacgcgct ctccggcatc ctcatgaaga tgagcgatgt caaggaaatc cgaacgcaga
     7861 acaatgttac cgcctatctg gtccaggact tctacgaacc gctcaaggag cgccattcgc
     7921 tggtca
//
LOCUS       DAORHF010000174         7893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8801_length_7893_cov_4.999872, whole genome shotgun sequence.
ACCESSION   DAORHF010000174 DAORHF010000000
VERSION     DAORHF010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8801_length_7893_cov_4.999872"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PKJ16_13235"
     CDS             <1..215
                     /locus_tag="PKJ16_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48000.1"
                     /translation="NGAEEELDASTLFYRDNCLYCHVCDGRMPARFHQSPSFQILERL
                     EETDDMFYVVICGKRIILKEKAGNEE"
     gene            complement(276..674)
                     /locus_tag="PKJ16_13240"
     CDS             complement(276..674)
                     /locus_tag="PKJ16_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48001.1"
                     /translation="MLQKIVVSLAIALFAIAGCTTKADQLKALYTQSIAANETCAAAL
                     EKAATPEEVAAAITAWGEESARIAAAQKQLQKTDPVKPDEEQFKKMMDAMQKQGKALV
                     ATLQKKEFLNNKTILDAYAKAAQKVADASK"
     gene            803..2941
                     /locus_tag="PKJ16_13245"
     CDS             803..2941
                     /locus_tag="PKJ16_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48002.1"
                     /translation="MVKQFLSTPGRRSAVCGILIMMMLGSPDTSPDALFAETVPDAHP
                     RRDRIAVRILSKQTRLLREGKTRQIDFILPSSAVLYDAGGHPHAVRLLRATVRDGQVV
                     VADMDRGREYAGSFSVSVRDPGGGLFSCALPGEARRYPFPLTIEGEPGGDIRVIVRES
                     RDDYCRSVAAAEYGPVPALEAEAVRALEMLIAARCDYAKKRAAHAGYDFCDLTHCHVY
                     RGRPLLSTGHAAQCDWSIDAGRMADSPLFHARCGGVLLDARVFTVGRASAPPLRDWIA
                     EENRYLCRSADSAWRCALPAREIVRLMFPKTSHPAPGSVRLSYDRSDLTISIRTDAAN
                     LRLAPEDFRLRINRVHGWNFIKSNNYSIEPIHVQGAPGFAFSGYGLGHGVGLCQRGAL
                     ALARLGYNRFEILEHYYPDIAWRAERSGSDDTPYAATMVFSLSTGALIAAPYPAFVER
                     TMAAGSFIKLLAALYCAAERPDLLAGHRFVCTGKTDGGDLPERCHDPAGHGDLDCAAA
                     LSRSCNIYFASLAGRIDAARFTAFSNALFARAGIGARVPAVADRREFARLLAGLDARM
                     RFSVRDCVALARLLSAGPVNDRGIESARQPIPPERRAMIARALQRTVIDGTAGTPLRP
                     YGSAINVTPLADGSANARRLQAAAASGSYWGKTSTVAEGSNRKHSYGMFVGGDDDRGV
                     ITILRKGNGHLAAKWSLVRLEALRAGEGAR"
     gene            2938..3855
                     /locus_tag="PKJ16_13250"
     CDS             2938..3855
                     /locus_tag="PKJ16_13250"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HNV48003.1"
                     /translation="MMRADGGIDRDALFAALNEGILDERAGAFRRNLARNRDAIRRHG
                     GLRTVVPLIEGRHVAIVGAGPSLEGALGPLGRYHHRQELCVIAADMALRPLARNGIRP
                     AFVISCETKPVDFFAGVDTTGMHLLAFSCMSSVNLRRWRGRVSFYNWMIHNDRYDPLW
                     RDAGEELGFVATGNLVTTQAVALALGCRIASLALFGNDLGFRRRYYAAETVRFHETLC
                     AVDRLLPLPTAEFRTARRAAEYALLRGNDAYCTNSQFLAAKLWLEELFSKQAVPIFDC
                     SDPGCSGSHAIKLAPKEYFARFDRKPRRR"
     gene            3860..4063
                     /locus_tag="PKJ16_13255"
     CDS             3860..4063
                     /locus_tag="PKJ16_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HNV48004.1"
                     /translation="MIRVHRLNGEEFILNANHIETVEERPDTTITMMNDRKYIVKEPA
                     VDVIAMVEEYNLRFYRAVHGKNE"
     gene            4357..4899
                     /locus_tag="PKJ16_13260"
     CDS             4357..4899
                     /locus_tag="PKJ16_13260"
                     /inference="COORDINATES: protein motif:HMM:NF015693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HNV48005.1"
                     /translation="MGDDEKPLVEEDEEESGARGEGEGQKSPTTSKIIKILLYVAGGI
                     LLIFIVFGISYLVSTYVQERRYAKEQDIVVVPPPAPLARYEIPVISFTTKDAEPHMGK
                     IALALGYDKNDQQLTTELTERAPQLQHIINVLLRGKSYEDINSVEGMINLTEDIKAHI
                     NVVLINGKIKEIYYKEFIVN"
     gene            5010..6020
                     /gene="fliN"
                     /locus_tag="PKJ16_13265"
     CDS             5010..6020
                     /gene="fliN"
                     /locus_tag="PKJ16_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HNV48006.1"
                     /translation="MLSTPSATPSFGAAGGGGSLSPLERDSLAELFNNTMRMVAPSLS
                     GYLSGKNITISNALIEVKPPDAVKADFNRQFVQVSMDYSGAVSGRNVILFNYADAGVI
                     SSLMMGDDSGTPPSELTDAHQSTIQEFVSQLVSSCATQFADRMGRGINTSPPALSIVN
                     NAAGVQLPAGNQIVKITYNLSIENLVSSKFYHLLDFGMASDLAKSTISGGQPTQQMYQ
                     QQMSQPQMMTGQVGISPVKFPPLGEGIPSGAAGNISLLLDVPMTLTVELGRTRQLVKD
                     ILGLGEGSIIELDKLAGEPVDLLVNGKLIAKGEVVVIDENFGVRVTDIVSPAERMSKM
                     QT"
     gene            6091..6939
                     /locus_tag="PKJ16_13270"
     CDS             6091..6939
                     /locus_tag="PKJ16_13270"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HNV48007.1"
                     /translation="MDETYSMRPVTGRAVCVVLLAAGIFFSFAIALCPIEATAKAKKS
                     AAKKSQRVEQTDDREEEAERRDVSSVTGSPVMAAEKSDDPTKKKEDPPKDAKEKAAAD
                     KGTIYDYEKPTAEEESYAWLIFKTLFILGLLVGGFYYFFRFVTKKAGVHVLGRDVVHV
                     LSVVPLAQNKFLQVVDLAGRIIVLGVSDNNINIITEITDRDEIDRIRLLSSKSSPVSE
                     NNFQDFLVKQLGSIGQLGRFFRRSTGGGRDGDTGYGGHDHDVDRLDYLRRQRERLKNL
                     NGKDHE"
     gene            7019..7702
                     /gene="fliP"
                     /locus_tag="PKJ16_13275"
     CDS             7019..7702
                     /gene="fliP"
                     /locus_tag="PKJ16_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421135.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HNV48008.1"
                     /translation="MRMPIPKVTFDIREATSAKDVALSFQILFLLTILTLAPSIIIMM
                     TAFTRVVIVLDFVKRALSLQQMPPNQVIVGLSIFLTIFIMAPTFTEINNKALQPYLKG
                     KISNEEFIDKGMEPMRTFMFKQTRKKDIALFIELAKLKKPANESEVPSYCLIPAFMIS
                     ELRRAFEIGVYIFIPFIVIDMIIASALMAMGMIMLPPVMISLPFKIVLFILVDGWNLL
                     AYQLVRSFG"
     gene            7719..>7893
                     /locus_tag="PKJ16_13280"
     CDS             7719..>7893
                     /locus_tag="PKJ16_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HNV48009.1"
                     /translation="MDDVLVIKILREALMVTMIVSAPILGVGMIVGLIISIFQTTTSI
                     QEQTLTFVPKIIAI"
BASE COUNT         1492 a         2381 c         2474 g         1546 t
ORIGIN      
        1 ccaacggcgc cgaggaggag ctcgacgcga gcaccctgtt ctatcgggac aactgcctgt
       61 actgccatgt gtgcgacgga cgaatgccgg cccggttcca tcaatcgccg tcatttcaga
      121 tcctcgagcg cctcgaggag accgacgaca tgttctatgt ggtcatctgc gggaaacgca
      181 ttatcctcaa ggaaaaggcg gggaacgagg aatgatgcgg ttcggtcgtt ggggcggggc
      241 attgcccgct ccctgcaacc gaaccgtgaa aaacattatt tcgatgcgtc ggcgactttt
      301 tgcgcggcct ttgcgtatgc gtccaggatg gttttgttgt tcaggaattc ctttttctgc
      361 agcgtggcca caagcgcttt cccctgcttt tgcattgcgt ccatcatctt tttgaactgc
      421 tcctcgtccg gtttcaccgg gtcggtcttc tgcaactgct tttgcgcggc ggcgatacgc
      481 gccgattcct cgccccacgc ggtgatcgcc gcggcgacct cttccggtgt tgcggctttc
      541 tcgagcgctg cggcgcaggt ctcgttggcc gcgattgatt gcgtgtacag cgccttgagt
      601 tggtcggcct tggtagtgca gccggcgatc gcgaaaaggg cgatcgcaag cgacacgacg
      661 attttttgaa gcatgcctgt tcctcctggt ggtataatcc cgcaaccctc ccgttcgttc
      721 gacgcactgt caagaggtat tccgaatacg atccatgcga cataatgtgc ttgacgatcg
      781 ccgtaacacg gtgtagcgga tcatggtgaa acagttctta tcgacaccag gcaggcgttc
      841 ggcggtgtgc ggcattctca tcatgatgat gctgggctcg ccggatacga gccctgacgc
      901 cctcttcgcc gaaaccgtgc cggatgcgca tccccggcgg gaccggatcg ccgtccgcat
      961 cctctccaag cagacgcgcc tgctccgaga gggtaaaacg cgccagatcg atttcatcct
     1021 tccttcgtcg gccgtgctgt atgatgcggg cgggcacccg cacgccgttc gtctcctgcg
     1081 agcgaccgtg cgcgacggac aggtggtcgt tgccgacatg gaccgtgggc gcgagtatgc
     1141 cggctcgttt tccgtgtcgg tgcgcgaccc cgggggaggg ctctttagtt gcgcgcttcc
     1201 cggcgaggcc cgccgctatc catttccgct tacgatcgaa ggagagccgg gcggcgatat
     1261 ccgggtgatc gtacgcgagt cgcgcgacga ctattgccgc tccgtcgcgg cggcggaata
     1321 cgggccggtt ccggcgctcg aggcggaggc ggtccgcgcc cttgagatgc tgatcgccgc
     1381 ccgctgtgat tacgcgaaaa agcgcgcagc gcacgccgga tatgatttct gcgacctcac
     1441 gcattgtcac gtgtatcgcg ggcggccgct cctatcaacc ggccatgcgg cccaatgcga
     1501 ctggtcgatt gacgcgggcc ggatggccga ttccccgctc tttcacgcgc gctgtggggg
     1561 cgtcctgctc gacgcgcggg tcttcacggt gggacgggcg tcggcgcccc cgctgcggga
     1621 ttggatcgcg gaggaaaacc ggtacctctg ccggagcgcg gattccgcat ggcgctgcgc
     1681 gcttcccgcg cgcgagatcg ttcggctcat gttcccgaag acatcgcacc ccgcgccggg
     1741 gtccgtgcga ctgagctatg accgatccga cctgacgatt tccatccgaa ccgatgctgc
     1801 gaaccttcgg cttgccccgg aggactttcg cctgcgcatc aatcgcgtgc atggctggaa
     1861 ttttatcaag agcaacaact attcgatcga gccaattcac gtccagggcg ctcccggctt
     1921 cgcctttagc gggtatggcc tggggcatgg cgtgggattg tgccagcgcg gcgcgctcgc
     1981 cctggcgcgg ctcggctata accgattcga aattctcgag cattattatc ccgacatcgc
     2041 gtggcgcgcc gagcgaagcg gatctgacga cacgccgtat gcggcaacga tggtcttctc
     2101 gctctcgacg ggagcgctca tcgccgctcc gtatcccgca ttcgtcgaac gaaccatggc
     2161 ggccggttcg ttcatcaagc tccttgcggc gctctattgc gccgccgagc ggccggacct
     2221 gctcgcgggc caccggttcg tctgcacggg gaaaacagac ggcggcgacc tccccgagcg
     2281 ctgccacgac cccgccggac acggcgatct cgactgcgcc gccgcgcttt cgcgttcgtg
     2341 caatatctat ttcgcctcgc tcgccgggcg catcgatgcc gcgcggttta ccgccttctc
     2401 gaatgcgctg ttcgcccgcg cggggatcgg cgcgcgcgtg ccggcggtcg ccgaccggcg
     2461 cgagtttgcg cggcttctgg cggggctcga tgcgcgcatg cgcttctcgg tccgcgattg
     2521 tgtcgcgctc gcgcggctcc tgtccgcggg ccccgtgaac gaccgcggca tcgaatccgc
     2581 gcggcagccg attcccccgg agcggcgcgc gatgatcgcc cgggcgcttc agcgcacagt
     2641 gatcgacggc accgccggaa cgccgcttcg gccatacggc agcgcgatca acgttacgcc
     2701 gcttgccgac ggctccgcca atgcgcgccg tttgcaggcc gccgccgcga gcggatcata
     2761 ctgggggaaa acctccaccg ttgccgaggg gtccaatcgc aaacacagtt acggcatgtt
     2821 cgtggggggc gatgatgacc gcggcgtgat caccatcctc agaaaaggga acgggcacct
     2881 tgccgcgaaa tggtcgctcg tccgtctcga ggcgcttcgg gcgggggagg gcgcgcgatg
     2941 atgcgcgcag acggcgggat cgatcgggac gcgctctttg cggcgctcaa cgagggcatc
     3001 ctcgacgagc gcgcgggcgc gtttcgccgg aacctcgcgc gcaaccggga cgccatacgc
     3061 aggcatggcg ggctgcgcac ggtggtgccc ctcatcgagg gacggcacgt ggcgatcgtc
     3121 ggggcaggcc cgtcgctcga gggcgcgctt gggcccctcg ggaggtatca ccatcgacaa
     3181 gagctgtgcg tcattgcggc cgacatggcg ctccggccgc tggcgcgcaa cgggatccgc
     3241 cccgccttcg tcatctcctg cgaaaccaag cccgtcgact tcttcgcggg cgtcgatacg
     3301 acaggcatgc acctgctggc gttttcgtgc atgtccagcg tcaacctgcg ccgctggcgg
     3361 gggagggtga gcttctacaa ttggatgatc cacaacgacc ggtatgatcc gctctggcgc
     3421 gatgcgggcg aggagctcgg cttcgtcgcc acgggaaacc tcgtgaccac gcaggcggtc
     3481 gctttagcgc tcgggtgccg gattgcgtca ctcgccctct tcgggaacga tttgggcttt
     3541 cgacgccgct actatgccgc cgagaccgtc cggtttcacg agacgctctg cgcggtcgac
     3601 cggttgcttc cgctcccgac ggcggagttc cggaccgccc ggcgcgccgc ggagtatgcg
     3661 ctcctccggg ggaatgatgc ctattgcacg aacagccagt tccttgccgc caagctgtgg
     3721 ctcgaggagc tgttttcgaa acaggcggtg ccgatcttcg attgcagcga tccggggtgc
     3781 tcggggagtc acgcaatcaa gctcgcgcca aaagaatatt ttgcgcgctt cgatcggaag
     3841 ccgcgacgga ggtaggacga tgatacgggt gcaccggctc aacggcgagg aattcatcct
     3901 caacgccaat cacatcgaaa cggtcgagga gcggccggat accacgatta ccatgatgaa
     3961 cgaccggaag tatatcgtga aagaaccggc cgtcgatgtg atcgccatgg tggaggagta
     4021 caatctgcga ttctatcgcg ccgttcacgg gaaaaatgaa tgatgattga aaaacaatat
     4081 aagtgtacaa aatatataaa aatatattga cactttattt ttaatatatc attatgtcac
     4141 ataactgtgc acagaaggtc gtttttaacg ggtttttttc gtcagcggca cgaaataaat
     4201 tatagtagta aaaattgtca ttgtgcggtg ccgataacat aagcaggaac gaaaatcggc
     4261 acccgatgat cgtggcggat ccgcgctcgt atcacatgtg atgccggtgt cgcgagtgcg
     4321 gcaatgcgcg gacaagtata atcggtgggg aaacacatgg gcgatgatga aaaaccctta
     4381 gtggaagagg acgaggagga gtcgggggcg cgcggcgaag gcgaagggca gaagagcccg
     4441 accacgtcga agatcatcaa gatcctgttg tacgtggccg ggggcatcct gctcatcttc
     4501 atcgtgttcg gtatttcgta tctcgtgtcc acctatgtcc aggagcgccg gtacgccaaa
     4561 gagcaggaca tcgttgtcgt gccgccgccg gcgccgctcg cgcgctatga gatcccggtg
     4621 atctcgttta cgaccaagga cgcggagccg cacatgggga agatcgcgct ggcgctgggc
     4681 tacgacaaga acgaccagca gctcaccacc gagctcaccg agcgtgcgcc ccagctgcag
     4741 cacatcatca acgtgcttct gcgcggcaag agctacgagg atatcaattc cgttgagggc
     4801 atgatcaatc tcaccgagga catcaaggcg cacatcaacg tggtcctcat caacggcaag
     4861 atcaaggaga tttactacaa ggagttcatc gtgaattgat ggtcgtcccg gtgcgctggg
     4921 gcggttgatt tatcgatctg ggggaaacca tgggtgacgg ctcactgtcg caagatgaaa
     4981 tagatgccct attacagggt gcggacgaca tgctttcgac cccgtcggcg acgccgagct
     5041 tcggagcggc cggcggcggg ggctcgctgt cacccctcga gcgcgattcg ctcgccgagc
     5101 tgttcaacaa caccatgcgc atggtggcgc cctcgctctc cggctacctg agcggcaaaa
     5161 acatcaccat atccaacgcg ctcatcgagg tgaagccgcc cgacgccgtg aaggccgatt
     5221 tcaaccgcca gttcgtccag gtgagcatgg actacagcgg cgcggtgagc ggtcgcaatg
     5281 taatcctttt caactacgcg gacgcgggcg tcatctcctc cctcatgatg ggggacgatt
     5341 ccgggacgcc gccgtcggag ctcaccgacg cgcatcagag caccatccag gagttcgtga
     5401 gccagctcgt ttcatcgtgc gccacgcagt ttgcggaccg aatggggcgg ggcatcaaca
     5461 ccagcccgcc ggcgctttcc atcgtgaaca atgccgccgg tgtgcagctg cccgccggaa
     5521 accagatcgt caagatcacc tacaatctct ccatcgagaa cctcgtaagc tcgaagttct
     5581 atcacctgct cgatttcggg atggcctccg acctggcgaa atcgaccatt tcgggcggtc
     5641 agccgacgca gcagatgtat caacagcaaa tgtcccagcc gcagatgatg accgggcagg
     5701 tgggcatcag cccggtgaag ttcccgccgc tgggcgaggg gatcccgtcg ggcgccgcgg
     5761 gcaatatatc gctgctgctc gacgtgccga tgacgctcac ggtggagctg ggccgcacgc
     5821 gccagctcgt gaaggacatc ctgggattgg gggagggttc catcatcgag ctggacaagc
     5881 tggccggcga gccggtggac ctcctggtga acggcaagct catcgcgaag ggcgaggtcg
     5941 tggtcatcga cgaaaacttt ggcgtgcgcg tgaccgacat cgtaagtccg gccgagcgca
     6001 tgtcgaagat gcagacgtaa gtgacgatcc ggacggcgct ttcgcggcgc ggtccgcacg
     6061 gacaggagac ataatcagga tatgatgcga atggacgaaa catattcgat gcggccggtg
     6121 acgggtcggg ccgtgtgcgt agtgctcctt gccgcgggta tatttttttc cttcgcgatc
     6181 gcattatgtc ccattgaagc caccgccaag gcaaaaaagt ccgctgcgaa aaagagccag
     6241 cgcgtggagc agacggatga tcgcgaggag gaggcggagc gtcgcgacgt ctcgtcggtg
     6301 acgggatcgc ccgtgatggc cgccgagaag agcgacgacc cgacgaagaa gaaagaggac
     6361 ccccccaagg acgcgaagga gaaggccgcg gccgacaagg gcacgatcta tgattacgaa
     6421 aaaccgacgg ccgaagagga gagttacgcg tggctcatct tcaagaccct ctttatcctg
     6481 gggcttttgg tgggcgggtt ttattacttc ttccggttcg tgaccaagaa ggccggcgtc
     6541 cacgtgttgg gacgagacgt ggtgcatgtg ctctcggtgg tgccgctggc ccagaacaag
     6601 ttcctgcagg tggtggacct cgctgggcgg atcatcgtgc tgggcgtgtc cgacaacaac
     6661 atcaacatca tcaccgaaat taccgaccgt gacgagatcg accgcatccg tctcctgagc
     6721 tccaagtcct ctccggtctc cgagaacaac ttccaggatt ttctggtcaa gcagctgggc
     6781 tccatcgggc agctcgggcg cttcttccgc cgctccacgg ggggcgggcg cgacggcgat
     6841 accgggtatg gggggcacga tcatgatgtt gaccggcttg actacctgcg ccggcagcgt
     6901 gagcggctga aaaacctcaa cgggaaagac catgaataag aaacggttga taatcggtct
     6961 ggtgacgctg atcgcgctgg cgatcccggc gctggcgctc gcgcaggacg tgggcgggat
     7021 gcgcatgccc atccccaagg tgaccttcga tatccgcgag gcgacatcgg cgaaggacgt
     7081 cgcgctctcg tttcagatac tgtttctgct cacgatcctc accctcgcgc cgtcgatcat
     7141 catcatgatg accgccttca cgcgcgtggt gatcgtgctc gacttcgtga agcgcgcgct
     7201 ctcgctgcag cagatgccgc ccaaccaggt gatcgtgggc ctctcgatct ttttgacgat
     7261 ctttatcatg gcgcccacct tcaccgagat caacaacaag gcgctgcagc cgtacctcaa
     7321 gggcaagatc agcaacgagg agtttatcga caagggcatg gagcccatgc gcaccttcat
     7381 gttcaagcag acgcgcaaga aggacatcgc gctgttcatc gagctggcca agctcaaaaa
     7441 gcccgccaac gagagcgagg tgccgtcgta ctgcctcatc ccggccttca tgatcagcga
     7501 actcaggcgc gccttcgaga tcggggtgta catcttcatc ccgttcatcg tcatcgacat
     7561 gatcattgcg agcgccctga tggcgatggg catgatcatg ctgccgccgg tgatgatatc
     7621 gctgccgttc aagatcgtgc tgtttatcct ggtggacgga tggaatctgc tcgcctacca
     7681 gctcgtcaga tcattcgggt aaagggagag ggctgagcat ggacgacgtg ctggtcatta
     7741 aaatacttcg ggaggcgctg atggtcacca tgatcgtttc ggcgcccatt ctgggcgtgg
     7801 ggatgatcgt ggggcttatc atctccatct tccagacgac cacctcgatc caggagcaga
     7861 cgcttacgtt cgttcccaag atcatcgcca tct
//
LOCUS       DAORHF010000175         7868 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8845_length_7868_cov_4.909126, whole genome shotgun sequence.
ACCESSION   DAORHF010000175 DAORHF010000000
VERSION     DAORHF010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8845_length_7868_cov_4.909126"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PKJ16_13285"
     CDS             <1..269
                     /locus_tag="PKJ16_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HNV48010.1"
                     /translation="NPETAKKFGKCEGSRVDLESQRGEVGSVRVHLTKTVAPDTVAVP
                     LGFGHRHYTAYGEDKGVNPKEIMCDDIDPVSGNADWWFTRIKLS"
     gene            312..1091
                     /locus_tag="PKJ16_13290"
     CDS             312..1091
                     /locus_tag="PKJ16_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV48011.1"
                     /translation="MAIDLDKCTGCGACSIACMQENNLPMFTDDSDSPKRVTFLDLMR
                     VTNDRDTDKKFGDVETAFIPKMCQQCEGNDPKNPKPPCVSVCPVVATDVGDDGVVSQI
                     WSRCIGCRYCQASCPYEARVFNWWKPDYEGSFKEGLSPDVSVPSRGTVIKCTFCSHIW
                     KRERDRMLAKGVKDINAVLYTPSCASACPTGAIIFGDLNDQASEVSRLAKDKRAFRLV
                     HTIDDKPEEEKKRLMAIKHYPNPKVYYLSSKQWIKDMQKFK"
     gene            1115..2425
                     /gene="nrfD"
                     /locus_tag="PKJ16_13295"
     CDS             1115..2425
                     /gene="nrfD"
                     /locus_tag="PKJ16_13295"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HNV48012.1"
                     /translation="MEILNQIKDEWNAAKTACLNGITGAMKFCKDEWNGYTRNIKITL
                     LVLFGLVGLFIIFGIQILGWGLGYTDMSNLYPFGQWIIGDLGLVGLGGGAFTMGFFLY
                     ILRVDKLKPLINSTVLIGFMCYLFTLVFLVFDIGQPLRAWFGYAYPNWGHGLMPQSML
                     TEVVWCLTFYFCVLIIELVPLPLEHPVLQKFPFIHGLTHYMHKLMWIMAAAGTFLSFF
                     HQGSLGGGMWGVLYGKVVWYRPAYFFTAIVAATAGGTAFMALCAYIAGKVMKKEVVPW
                     DSFQTLAQISGVLTIFAFLFQAWHVYMQATHFVPAFDRSFLDIWGGYYGIWMVVLMFA
                     AYIGAIVLLNVGALRSQERFFVAGISCGVGAISFDKMIVVLHGSSTPNFPWKSFAGYN
                     PTIQEWGIFLGGLATMILIYMLFAKYFPLFPHLNKHGHGEEHAH"
     gene            2579..2920
                     /locus_tag="PKJ16_13300"
     CDS             2579..2920
                     /locus_tag="PKJ16_13300"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HNV48013.1"
                     /translation="MSDTQLITIACVIAGVLEIALGFPLFKGKVKPNGIYGFRTPTTL
                     ASEKVWYAANRLVGKDLVICGATLAVGSSLLYERAGSLSLVQTAGIELALAIVPLALM
                     LWRASAYLKRL"
     gene            complement(2925..3302)
                     /locus_tag="PKJ16_13305"
     CDS             complement(2925..3302)
                     /locus_tag="PKJ16_13305"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNV48014.1"
                     /translation="MSNAVAYLSLVGTTACAVCGQMIIKWRINAIPHFPAALPERLWF
                     IVRLLFDPYIIAGFAVGLVGLVLWLNALSKLHLSYAYPFMGMNFVLIFVMSALVFGEP
                     VTIHKIIGAALIVAGLIVSSQSI"
     gene            complement(3299..4189)
                     /locus_tag="PKJ16_13310"
     CDS             complement(3299..4189)
                     /locus_tag="PKJ16_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HNV48015.1"
                     /translation="MATIIDGNAIAAEIRGEIKNEVAAFVREGRTAPGLAVVLVGNDP
                     ASEVYVRNKGKDCVEVGITPHQHTLPAATTQDELIRLIDELNERSDVNGILVQLPLPK
                     HINENTVLNRINPWKDADGFHPVNVGKTVIGESDAFFPCTPFGCQVMINRALRDIAGK
                     HCVVVGRSNIVGKPIANMMVQKNKQANCIVTVCHTAAPDISYYTKQADILVVAAGRAR
                     FVTGAMVKEGVVVIDVGINRVDDPKNPGKTRLVGDVDFDSVEPKASAITPVPGGVGPM
                     TRAMLLKNTMRAYRLQNGIA"
     gene            complement(4285..4731)
                     /locus_tag="PKJ16_13315"
     CDS             complement(4285..4731)
                     /locus_tag="PKJ16_13315"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HNV48016.1"
                     /translation="MTTPLPHFELVENLSMADVAFRAYGSSPGELFRSASLAVLSVLL
                     ENPLCVAASESVPLSLRNAHLDLLLFDFLNELIYRKDVDAVLLYATHAVVECAGSEWT
                     LDARLAGERIDRGKHVFAVDIKAATMHRLSVENNNGLWVATVVLDV"
     gene            complement(4728..6182)
                     /locus_tag="PKJ16_13320"
     CDS             complement(4728..6182)
                     /locus_tag="PKJ16_13320"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HNV48017.1"
                     /translation="MRIEALNKISDNCWEIPKRYKQGMRVPGRIYASEELVRGMDNAV
                     LDQTANVAMLPGIQNYALCMPDGHSGYGFPIGGVAAIDPDEGVISPGGIGFDINCGIR
                     LIATSLTRAEVEPHVANIVDSLFRRIPAGVGAKGMLALGDRTFDAAMISGARWALENG
                     YAEPGDLAVIEEGGVMAGADPAAVSAKARERGRIQVGTLGSGNHFLELQCVRREHVFD
                     VAAARRFGIDRDDQVFFMIHCGSRGFGHQIATDYLARFLAAMGPKYGIALPDRELACA
                     PFRSPEGRDYFAAMCCAINIAFLNRQVIMHCLREVLADVFRRSPADLGVRLVYDLCHN
                     TAKLERYEIDGREKTLLVHRKGATRALCAGMPGVPDAYRDVGQPVLIGGSMESGSYLL
                     VGEPSARETFFTTAHGSGRVMSRGQAKRSFNGRELQAQMKRKGIYVQTASFPGLAEEA
                     GGAYKDIDAVVAATHAAGLSRPVAKLTPMGNIKG"
     gene            complement(6218..7210)
                     /gene="amrS"
                     /locus_tag="PKJ16_13325"
     CDS             complement(6218..7210)
                     /gene="amrS"
                     /locus_tag="PKJ16_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV48018.1"
                     /translation="MEREARHYSAESGSAVRCHLCPHECRIAAGAAGTCGVRINRDGK
                     LFSAIYGEVTSVAMDPIEKKPLYHFHPGSQILSIGTKGCNFRCAFCQNWSISQDLNAR
                     TAAYRPEDIVQAARTNNSIGIAYTYSEPIIWFEYVLECARLAREAGLKNVMVTNGFVN
                     PAPRAELLELIDAMNIDLKTFNDDTYRRIPHGRLQPVLDTIVAAHAAAHVEITTLVVT
                     GMNDTMEEMRAIIDWIAALDPGIPWHISRYHPSYKYDAPATDVDFMLRVHEAARKKLH
                     HVYCGNIANGRGTSDTHCPSCGAVLVARSGYATRITGISNGACVKCGRASGIVQ"
     gene            complement(7213..7629)
                     /locus_tag="PKJ16_13330"
     CDS             complement(7213..7629)
                     /locus_tag="PKJ16_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV48019.1"
                     /translation="MAKRIIQINKNPVYEFGMISTVFGKDTQFCGDLHFRKSLQINGT
                     FEGEIASEGYLVVGEGAVVKANVKANTVIIKGTVHGNIEARDRLEINPGGKLYGNIRT
                     AKLKIADNVVFQGNCEMIKKHEKQEKGEAERAESAS"
     gene            complement(7658..7855)
                     /locus_tag="PKJ16_13335"
     CDS             complement(7658..7855)
                     /locus_tag="PKJ16_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48020.1"
                     /translation="MLRLYNDGLAAYKQRKWDEAIRAFEGALKVDPNDGPSRTYLERS
                     NEFRQSPPPEDWDGVYVMKTK"
BASE COUNT         1468 a         2515 c         2333 g         1552 t
ORIGIN      
        1 tgaacccgga aacagcgaag aaattcggga aatgcgaggg ctctcgcgtg gacctcgaat
       61 cacagcgcgg cgaggtgggc tccgttcgcg tgcacctcac caagaccgtc gccccggaca
      121 cggtcgccgt tccgctcgga ttcggccacc ggcactatac cgcatatggc gaggacaagg
      181 gcgtaaaccc caaggaaatc atgtgcgacg atatcgatcc cgtatccggt aacgccgact
      241 ggtggtttac caggatcaaa ctgagctaag gagcagaacg accaatggca acaatgaaac
      301 gtaagtgggg aatggcgata gatcttgata aatgcaccgg ctgcggggca tgttcgatcg
      361 cgtgcatgca ggaaaacaac ctgcccatgt tcacggacga ttcggactcg cccaagcggg
      421 tcaccttcct ggacctcatg cgcgtcacca acgaccgcga cacggacaag aagttcggcg
      481 acgtcgagac cgccttcatc cccaagatgt gccagcagtg cgaagggaac gaccccaaga
      541 acccgaagcc cccgtgcgtg tcggtatgcc ccgtcgtggc caccgacgtc ggcgacgacg
      601 gcgtggtgag ccagatctgg tcgcgctgca tcggttgccg atactgccag gcgtcgtgcc
      661 cgtacgaggc gcgcgtgttc aactggtgga aaccggacta cgagggctcc ttcaaggagg
      721 gactcagccc cgacgtatcg gtcccctcgc gcggcaccgt gatcaagtgc accttctgca
      781 gccacatctg gaagcgtgag cgcgaccgca tgctcgccaa gggcgtcaag gacatcaacg
      841 ccgtcctcta caccccgagc tgcgcatcgg cctgccccac cggcgccatc atcttcggcg
      901 acctcaacga tcaggcgtcc gaagtgtccc gcctggccaa ggacaagcgc gcgttccgcc
      961 tggtgcacac catcgacgac aagcccgaag aggaaaagaa gcgcctgatg gcgatcaagc
     1021 actatccgaa tcccaaggtt tactaccttt ccagtaagca gtggatcaag gatatgcaga
     1081 agttcaaata atcctgagac aaggaaggga ttcaatggaa atactcaatc agatcaaaga
     1141 cgaatggaac gccgcgaaaa cggcctgttt aaacggcatc accggcgcga tgaaattctg
     1201 caaagacgag tggaacggct acaccagaaa catcaagatc acgctcctgg tcctcttcgg
     1261 gcttgtgggg ctgttcatca tctttggcat ccagatcctc ggctggggcc tcgggtacac
     1321 cgacatgagc aacctgtacc ccttcggcca gtggatcatc ggcgacctgg gtctcgtggg
     1381 actgggcggc ggcgcgttca ccatgggatt tttcctctac atcctgcgcg tggacaagct
     1441 gaagccactc atcaactcca ccgtgctcat cgggttcatg tgctacctgt tcaccctggt
     1501 gttcctggtg ttcgatatcg gccagccgct ccgcgcatgg ttcggctacg cctaccccaa
     1561 ctggggacac ggtctcatgc cgcaatcgat gctcaccgaa gtggtttggt gtctcacctt
     1621 ctacttctgc gtgctcatca ttgagctggt gccgctcccg ctggagcatc cggtcctgca
     1681 aaagtttccc tttatccatg gcctcacgca ctacatgcac aagctcatgt ggatcatggc
     1741 tgcggccggc accttccttt ccttcttcca ccagggatcg ctcggcggcg ggatgtgggg
     1801 cgttctatac ggcaaggtgg tctggtatcg cccggcatat ttcttcacgg cgatagtcgc
     1861 cgcgacggcc ggcggcacgg ccttcatggc cttgtgcgcc tacatcgccg gcaaggtcat
     1921 gaagaaggaa gtggtgccgt gggactcctt ccaaacgctt gcgcagattt ccggcgtact
     1981 caccatcttc gccttcctgt tccaggcgtg gcacgtctac atgcaggcga cccatttcgt
     2041 ccccgcattc gaccggagtt tcctcgacat ctggggcggc tactatggga tctggatggt
     2101 ggttctcatg ttcgccgcct atatcggagc gatcgtgctg ctcaatgtcg gagcgcttcg
     2161 ctcacaggag cgcttcttcg tggcggggat atcctgcgga gttggcgcaa tctccttcga
     2221 caagatgatc gtggtgctgc atggatcgtc cacccccaac ttcccctgga agtcgttcgc
     2281 cgggtacaac ccgaccatcc aggaatgggg gatcttcctc ggggggctgg ccaccatgat
     2341 actcatctac atgctcttcg cgaagtactt cccgctcttc ccgcacctga acaagcacgg
     2401 gcacggcgag gaacacgcgc attagaaatt ctatttaaat aatgcacccg aaacgggagc
     2461 gagttatcgc tcccgttttt tgtttgatta ttgcatgcca tcaagcaaat aatgcatagc
     2521 atgatggtat ggaacacaca ggtaatcgaa aaattcgttc cgccccggag agcacgacat
     2581 gtccgacaca caactcatta caatagcatg cgtgatcgcc ggcgtcctcg agatcgcgct
     2641 gggttttccg ctgttcaagg gaaaagtgaa accgaacggc atctacgggt tccgcacgcc
     2701 cacgacgctc gcgagcgaga aggtctggta cgcggcgaac cggctcgtcg ggaaggacct
     2761 ggtgatctgc ggcgcgacgc ttgcggtggg ttcctcgctc ctctatgagc gcgccggctc
     2821 gttatcactc gtgcagaccg cgggcatcga gctcgccctc gcgattgttc cgctcgcgct
     2881 catgctatgg cgcgcctccg cctacctgaa gcgattgtag tcgatcagat cgattggctc
     2941 gaaacgatca ggccggcaac gataagcgcc gcgccgatga tcttgtgtat ggtcaccggc
     3001 tcgccgaaca ccagcgccga catcacgaaa atcaggacaa agttcatgcc cataaacgga
     3061 taggcgtaac tgaggtgaag cttcgacaat gcgttgagcc acagaacgag gcccaccaat
     3121 ccgaccgcga aaccggcaat gatataggga tcgaagagaa gccgtacgat gaaccacaac
     3181 cgttcgggca gagcggccgg gaaatgcggg atcgcgttga tccgccactt gatgatcatc
     3241 tgcccgcaaa ccgcgcacgc ggtcgtcccg accaggctga gataggcgac ggcgttgctc
     3301 acgcgatccc gttttgcaac cggtacgcgc gcatcgtgtt cttgagaagc atcgcgcgcg
     3361 tcatggggcc cacgccgccg ggaaccgggg tgatggccga ggccttcggc tccaccgaat
     3421 cgaaatccac gtcgcccacc aggcgcgttt tgccggggtt cttcgggtcg tcgacgcggt
     3481 tgatgccgac gtcgatcacg accaccccct ccttgaccat ggcgccggtg acgaagcgcg
     3541 cgcgccccgc ggcgaccacc aggatgtcgg cctgcttggt gtagtacgat atgtccggcg
     3601 cggcggtgtg gcacaccgtg acgatgcagt tcgcctgctt gttcttctgc accatcatgt
     3661 tcgcgatggg cttgcccacg atattgctcc gccccaccac cacgcagtgc ttgccggcga
     3721 tatcgcgcaa cgcgcgattg atcatcacct ggcacccgaa gggcgtgcag gggaaaaagg
     3781 cgtccgactc gccgatgacg gtctttccga cgttcaccgg atggaacccg tccgcgtcct
     3841 tccatgggtt gatacggttg agcaccgtat tctcgttgat gtgcttcggg agcggaagct
     3901 gcaccaggat cccgttcacg tcgctgcgct cgttcagctc gtcgatgagg cggatgagct
     3961 cgtcctgcgt ggtcgctgcc ggcagggtgt gctggtgggg ggtgatgccg acctctacgc
     4021 agtccttgcc cttgttgcgc acgtacacct cagaggccgg atcgttgccc accagcacca
     4081 ccgcgagtcc cggcgccgtc cgcccctcgc gcacgaacgc cgcgacctcg ttttttatct
     4141 cgccgcgtat ctcggcggcg atagcattgc catcgattat tgttgccatg acgactccat
     4201 tgaacgcctg gacatttggt atcaccttcg cggaatcgca ttccaatgcg gtggcatgcg
     4261 ccgaataatg aaaggtgtcg cgaactacac gtcgagcacg accgtggcga cccacaaccc
     4321 gttgttgttc tcgaccgaca accgatgcat ggtggccgcc ttgatgtcaa ccgcaaatac
     4381 gtgttttccc cggtcgatcc gctcgccggc aagccgcgca tcgagcgtcc actccgatcc
     4441 cgcgcattcc acaacggcgt gggttgcata cagaaggacc gcatcgacgt cctttcgata
     4501 gatgagctca ttgaggaaat cgaagagcaa cagatcgagg tgggcgtttc gcaacgagag
     4561 gggaaccgat tccgacgcgg cgacgcagag cggattctcc agcagcaccg agagaacggc
     4621 gagcgaggcc gacctgaaga gctcgcccgg actcgacccg taggcgcgaa aggcgacatc
     4681 ggccatcgac aggttctcga cgagctcaaa atgcggaagc ggggtggtca tcccttgata
     4741 ttacccatcg gcgtgagctt ggccaccggg cgcgaaagcc cggcggcgtg cgtcgcggcg
     4801 acaaccgcgt cgatgtcctt gtaggcgccg cccgcctcct cggcaaggcc ggggaatgac
     4861 gcggtctgca cgtagatgcc cttgcgcttc atctgcgcct gcagttcccg gccgttgaat
     4921 gaccgcttcg cctgcccgcg cgacatcacc cggccgctgc cgtgcgccgt cgtgaagaag
     4981 gtctcgcgcg cgctcggctc gccgacgagc agatacgatc cgctttccat gcttccgccg
     5041 atgagcaccg gctgccccac gtcccgatac gcgtccggca cccccggcat ccccgcgcat
     5101 aacgcgcgcg tggcaccctt ccggtggacc agcagcgtct tctccctgcc gtcgatctcg
     5161 tagcgctcga gtttggccgt attgtggcac agatcgtaca ccagccgaac ccccagatcg
     5221 gcggggctcc tgcggaacac gtcggccagc acctcgcgca ggcagtgcat aatcacctgg
     5281 cgattgagaa aggcgatgtt gatggcgcaa cacatggcgg caaaatagtc ccggccctcg
     5341 ggcgaacgga agggcgcgca ggcgagctcc cggtcgggaa gcgctatgcc gtacttcggt
     5401 cccatggccg cgaggaaccg ggccaggtag tcggtcgcga tctggtggcc gaacccgcgg
     5461 ctcccgcagt ggatcataaa aaaaacctgg tcgtcgcgat cgatgccgaa tcgccgcgcc
     5521 gccgcgacgt cgaacacgtg ctcgcgacgc acgcactgaa gctcgaggaa atggttgccc
     5581 gaccccagcg tccccacctg gatcctcccg cgctcgcgcg ccttcgcgct caccgcagct
     5641 ggatcggcgc ccgccatgac gcccccctcc tcgatgacgg cgagatcgcc cggctcggcg
     5701 tatccgttct caagggccca gcgcgctccc gagatcatcg ccgcgtcgaa tgtccggtcg
     5761 ccgagcgcca gcatcccctt cgcgcccacg ccggcgggga tccgcctgaa gagcgaatcg
     5821 acgatgttcg cgacgtgcgg ctcaacctct gcgcgcgtaa gcgaggtcgc gatgagcctg
     5881 atgccacagt tgatgtcgaa tccgatgccc cccggcgata tcaccccctc gtccggatcg
     5941 atggccgcga cgccgccgat ggggaagcca tagccgctgt ggccgtcggg catgcacagc
     6001 gcatagttct gtatgcccgg cagcatcgcg acgttcgcgg tttgatcgag caccgcattg
     6061 tccatgccgc ggacgagctc ctcggacgcg tagatccggc cgggaacgcg catgccctgc
     6121 ttatagcgtt tcggaatctc ccagcagttg tcgctgattt tattgagcgc ttcgatccgc
     6181 attcagtgcc cccgttgcgc cggccgggcg gggccggtca ctgtacgata ccgctcgccc
     6241 gtccgcactt cacgcacgcc ccgttggaaa tccccgttat gcgcgttgcg tacccgctgc
     6301 gcgcgaccag cacggcgccg caggagggac agtgcgtgtc gctggtgccg cgtccgttcg
     6361 cgatattgcc gcaatagaca tggtggagct tctttcgcgc ggcctcatgc acgcgcagca
     6421 tgaagtcgac gtcggtcgcg ggcgcgtcgt atttgtagct cggatgatag cgcgatatgt
     6481 gccaggggat ccccgggtcc agcgcggcga tccagtcgat gatcgcgcgc atctcctcca
     6541 tggtgtcgtt catgccggtc accacgagcg tggtgatctc gacatgcgcc gcggcatgcg
     6601 ccgcgacgat cgtatcgagg acgggttgca ggcgcccgtg cgggatgcgc cggtaggtgt
     6661 cgtcgttgaa ggtcttgagg tcgatgttca tcgcgtcgat gagttccagc aattcggcgc
     6721 gcggggccgg attcacgaag ccgttggtca ccatgacgtt cttcagtccc gcctcgcggg
     6781 cgagccgggc gcactcgagc acatactcga accagatgat cggctcggaa taggtgtagg
     6841 cgattccgat cgagttgttc gttcgggccg cctgaacgat atcctcgggg cggtatgcgg
     6901 cggtgcgcgc gttcagatcc tgcgagatgc tccagttctg gcagaatgcg cagcggaaat
     6961 tgcacccctt ggtgccgatg gagagaatct gagagcccgg atggaaatga tagagcggct
     7021 ttttctcgat ggggtccatc gcgacggagg tcacctcgcc gtagatggcg ctgaagagtt
     7081 tcccgtcgcg gtttatgcga acgccgcagg tgccggccgc tcccgccgcg atgcggcact
     7141 cgtgcgggca aagatggcac cggaccgccg agccggactc ggcggaataa tggcgcgctt
     7201 cacgctccat cgctacgagg ccgattcggc gcgttcagcc tcccccttct cctgcttctc
     7261 gtgctttttg atcatctcgc aatttccctg gaagaccacg ttgtccgcga tcttcagctt
     7321 cgcggtgcgg atatttccgt agagcttgcc tccgggattg atctccagcc ggtcgcgcgc
     7381 ctcgatgttg ccgtgtacgg tccccttgat gatcaccgta ttggccttga cgttggcctt
     7441 gacgacggcc ccctcgccca cgacgaggta cccctccgag gcgatctccc cctcgaaggt
     7501 gccgttgatc tgcagcgatt tcctgaaatg caggtccccg cagaactgcg tgtccttccc
     7561 gaacaccgtg gatatcatcc cgaattcgta tacggggttc ttgtttattt gaatgatcct
     7621 ttttgccatg ttccagtcct tgcccgcggt ctgcccgcta tttcgttttc atcacataca
     7681 cgccgtccca gtcttcgggc ggcggcgact gccggaattc gttcgaccgc tcgaggtagg
     7741 tccgcgacgg cccgtcgttc ggatcgacct tcaacgcccc ctcgaaggcg cggatcgcct
     7801 cgtcccactt tcgctgcttg tacgcggcca gtccatcatt gtagagccgc agcagttcct
     7861 ctttttcc
//
LOCUS       DAORHF010000176         7865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_8852_length_7865_cov_7.396031, whole genome shotgun sequence.
ACCESSION   DAORHF010000176 DAORHF010000000
VERSION     DAORHF010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7865
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8852_length_7865_cov_7.396031"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PKJ16_13340"
     CDS             <1..667
                     /locus_tag="PKJ16_13340"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV48021.1"
                     /translation="LNDKNQVVRIQSIRTMGAIKDRYSIKHLIDCLDDKKFQIRLEAT
                     KALGAVGGEVCVDPLIGSLVDNHGEVRRAADAALRQVGTEKALNAVTDAPFMLLVKKM
                     TENESVRLETVINIGRLKIKEGAPLLHKSCMDEYKNIRLESLRAVGQLRDKTAVDTVN
                     KLVEDPFYDVRLEAVKTLEKLFHPGALRGIEKAMKDTNKNVRLEAIRAYNSLKTRLEK
                     AAE"
     gene            667..1449
                     /locus_tag="PKJ16_13345"
     CDS             667..1449
                     /locus_tag="PKJ16_13345"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HNV48022.1"
                     /translation="MLLGANIGNTNTTLGFFHDDDPAPHATRRYATRKNATPDDLIRD
                     IEAALAAYRAESGSTDAVGALACSSVVPELNAAYHAAARSLCSCDAFDVSHAIRLNVR
                     LCYDDPSQLGADRIANAAAARREYASDCVIVDLGTAITFCVLRANGDFEGGLIAPGAG
                     ICIDALAERTSRLPRAEFRDPGTPIARNTADAVAAGVFYGWLSLVEGVVQRIERSCGT
                     AFTTVLTGGYADIIAAHATLDARVDPHLTLKGIKLLYDLNRA"
     gene            2051..2512
                     /locus_tag="PKJ16_13350"
     CDS             2051..2512
                     /locus_tag="PKJ16_13350"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HNV48023.1"
                     /translation="MDKNDRYSRYIARSVDHIFKNFIKDDTITEVFEAQSHENDPRVC
                     IELAGTISGEIVINIPEKTLSQLTKKIMPSVSPRAVRKHHREIAGEIANMITGTFANQ
                     LQFIKHNVRLSAPEFNEDPIALKTFYENVNLSFQSAFGGFDIDLYYKESAQ"
     gene            2516..4255
                     /locus_tag="PKJ16_13355"
     CDS             2516..4255
                     /locus_tag="PKJ16_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012474545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HNV48024.1"
                     /translation="MLTFLDIALIAVYFIVTVAIGLWYARRAGRSTEEYFLSGRKLPW
                     WIAGTAMVATTFAADTPLAVTELVLTHGIAGNWLWWNMVAGSVLTVFFFAHLWRRSAI
                     TTDVEFIELRYAGRPAAFLRGFKAVYLGVFMNCIIMGWVNVALAEILTHIFGLTTLQV
                     FAAVVSCMLCVGAYSALSGLWGVAITDMFQFVVAMAGCIVLAIIVLSVPQVGGIAGLA
                     ASLPGAMFSFVPDFGLSPAAAASGVTALAGSAFAAYALIQWWASWYPGSEPGGGGYVA
                     QRMMSAKDERHAILATLWFVIAHYAVRPWPWILVALAAAVLYPGSGKSGYILAMRDHL
                     PAGLLGFLVAAFLAAYMSTIATHLNWGTSYLMNDLYRRFIRVDASERHYVRVSRAITI
                     LLTAVSSSLIFVISSISGAWGFVIEFGAGLGLVLILRWYWWRPNAWSEITAMVAPFGG
                     WAISHFALGLSFPVSMYVIVPVTTAAWLGVTFLTAPTEDARLVAFYRRVRPAGPGWSG
                     FARRHGLEPSDGSIAALAINWLVGVVMIYAALFASGNIIFGKYGAGFALAAVAAASFA
                     FLARRIGMNERVS"
     gene            4479..5024
                     /locus_tag="PKJ16_13360"
     CDS             4479..5024
                     /locus_tag="PKJ16_13360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48025.1"
                     /translation="MNEYTATIAACYARHSNAVLDFLSRFLRDRHAAEDLTQETFIRL
                     LNRKEALDPDHPTALSYLLTIARHIACDHLRHQGVERRYQTMLQMEEVTLNERFFNDV
                     ADAHLEGEVISTMHDAIDSMPPRARSLILGKFYQRKKNNRLMRELGISNFILQRTLRE
                     AMTALRELLRAHYRPSEPSSN"
     gene            5090..6328
                     /locus_tag="PKJ16_13365"
     CDS             5090..6328
                     /locus_tag="PKJ16_13365"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HNV48026.1"
                     /translation="MSTNSRPSISVEDAIDIIIRSTAHLEPETVSLVDAGGRILAEDI
                     VAGADIPAFDNSAMDGYAVRAADITGATRDRPARLAVRGEVRAGGDLLRLDDPESAVR
                     IMTGAPIPPGADTVVMVEDTAETDGAVSIYDAVPMGSNIRRAGEDIPRGAVTLTAGER
                     LRASEIGVLASLNASTVRVARRPRVAIISTGDEVIEVGAPARDGVVRNSNAYILREEV
                     RRTGGEPTYLGIAPDTLEATRDLLARASEHDVIITSGGVSMGRYDFVRDALASIGFDI
                     SIESILMKPGKPCVFGRRDSTLFFGLPGNPVSTQVAFLQFVRPALLALMGARKRDKPL
                     VRAILEEPIDKKPGRVHFIRGVFTIENGEFRVRTTGPQGSGILRSMSRANCLIILPAD
                     MRRAERGDRVTIQLIDHDEV"
     gene            6328..7326
                     /locus_tag="PKJ16_13370"
     CDS             6328..7326
                     /locus_tag="PKJ16_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48027.1"
                     /translation="MAPRTGILLYSGGLDSLLAARILLDQGIELIGLHFVLPFFSPHA
                     DPADTAAARQAARIGLSLRHIRCGRDYMDMVERPAHGYGKHINPCIDCKIFFLQRARE
                     EMERTGAAFVATGEVVGQRPMSQMRHMLNHIEHASGLDGYLLRPLSARLLKPTVAEDE
                     GIVDRERLLDISGRSRRRQMELAARYEIEDYASPAGGCLFTDANYERRLRDLFAHRPD
                     YDLAAVYLLSVGRHFRLNERTRLIVARNEREGSALERARDSADCLLVPDFKGPTALLT
                     GDVDEATLALAGSIMLRYGRPEGINAIQVFRRGDDPRTMTIARPIGEDELKRAALA"
     gene            complement(7375..>7865)
                     /locus_tag="PKJ16_13375"
     CDS             complement(7375..>7865)
                     /locus_tag="PKJ16_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HNV48028.1"
                     /translation="MKFALNGALTVGTLDGANVEIMEEVGSENIYIFGLTADQVVSTR
                     ITGYNPWDYYHRNPELKRVVDMIGGGAFSPGQPGLFAPIVDSLLAQGDHYLLMADFES
                     YLNCQDRVARDYLDRPGWVRKSILNAARIGKFSSDRTIKEYADEIWKAKPVRVKIRPD
                     AT"
BASE COUNT         1407 a         2689 c         2341 g         1428 t
ORIGIN      
        1 gctcaacgac aagaaccagg tggtgcgcat ccagtcgata cgcaccatgg gcgccatcaa
       61 ggaccggtac tcgatcaagc acctcatcga ctgcctggac gacaagaagt tccagatccg
      121 cctcgaggcg accaaggcgc tgggcgccgt cggcggggag gtgtgtgtcg atccgctcat
      181 cggttcgctc gtggacaatc acggcgaggt gcgccgggcc gccgacgccg cgctccggca
      241 ggtcggcacc gaaaaggcgc tcaacgccgt gaccgacgcg ccgttcatgc tgctggtgaa
      301 aaagatgacg gagaacgagt ccgtccgcct cgagaccgtc atcaacatcg gccggctgaa
      361 gatcaaggag ggcgcgccgc tgctccacaa atcctgcatg gacgagtaca agaacatccg
      421 cctggagtcc ctgcgcgccg tcggccagct ccgcgacaag acggccgtcg atacggtcaa
      481 caagctcgtc gaggacccgt tctacgacgt ccgcctggag gccgtaaaaa cgctcgaaaa
      541 gctcttccat cccggcgcgc ttcgcggcat cgagaaggcg atgaaggaca ccaacaagaa
      601 cgtccgtctg gaggccatcc gggcgtacaa ctcgctcaag acgcgcctgg aaaaagccgc
      661 ggagtgatgc ttctcggcgc gaatataggc aacaccaaca ccaccctggg attttttcac
      721 gacgacgatc ccgcgccgca tgcgacacgg cgctatgcga cccgaaaaaa cgcaaccccc
      781 gacgatctga tccgcgacat cgaagccgcc ctcgcggcgt atcgcgcgga gagcggctcc
      841 actgacgccg taggcgccct ggcctgttcg agcgtggtcc cggagctcaa cgccgcctat
      901 catgcggccg cacgctcgct ctgttcctgc gatgcattcg atgtctcgca cgcgatccgc
      961 ctaaacgtcc gcctgtgcta cgacgacccg tcccagctcg gcgccgaccg catcgcgaac
     1021 gccgccgcgg cccgccgcga gtacgccagc gattgcgtga tcgtggacct ggggacggcc
     1081 atcaccttct gcgtccttcg cgcgaacggc gatttcgagg gcggcctcat cgcgccgggc
     1141 gcggggatct gcatcgacgc gctggccgag cgcacctcgc gcctgccccg cgccgagttc
     1201 cgcgatccgg gcacgcccat cgcgcgcaat acggcggacg cggtcgcggc gggcgtcttc
     1261 tatggctggc tttcgctggt ggagggcgtg gtgcaacgca tcgagcgcag ctgcggcacc
     1321 gccttcacca ccgtgctcac cggcggctat gcggacatca tcgccgccca cgccaccctt
     1381 gacgcgcgcg tcgacccgca tctcaccctg aagggcatca agctcctcta cgatctcaat
     1441 cgcgcctaat ccgtcggcat acgtcatcat cgccatttca tccaacgcga aaaaccgccc
     1501 atgcatcgtc gtattgccga taacgtgacc gacgcatccc ggttgcacat gcagcgtgag
     1561 actatgtgta gcgatattta tcatcataaa tgagagaatg cctatttatt tatcaaactt
     1621 gtccacaata ttatccacag tgtccacaat tatatatata atttaaatat tttgataaag
     1681 aaatttcact aagcaacttt tgcattgtgg gcctgacgcc tccgatgaga cataaaatat
     1741 gcacggcctg acggcatcag cacttagccg taaaaaaaat ttcttctgcg acccggagcg
     1801 cctcatttta tattgacatt cgaatccggc ggcccgatcc cgccgggcgg cacattcgcg
     1861 gaatatgtca ccttgtgaac aactgacaca tcggccaatt atgtgacata attctaaatc
     1921 ggcgggcgag aaatccttcc ggccccgaaa attgcgcgcg ggcgagcgaa aaggcttgat
     1981 atttctccac ggccatcgca caacggaacg gcactccggc gcaatcgccg aaccgaaacc
     2041 ggcaggatcc atggacaaga acgaccgcta ctcccgatac atcgcgcgct cggtggacca
     2101 catcttcaag aacttcatca aggacgacac catcacggag gtgttcgagg cgcagtcgca
     2161 cgagaacgat ccgcgcgtct gcatcgagct cgcggggacc atctccggcg agatcgtcat
     2221 caacatcccc gagaagacgc tgtcgcagct caccaaaaag atcatgccgt cggtgagccc
     2281 ccgcgcggtg cgcaagcacc atcgcgagat cgccggcgag atcgcgaaca tgatcacggg
     2341 gaccttcgcc aaccagctcc agttcatcaa acacaatgtc cgcctctcgg cccccgagtt
     2401 caacgaggac cccatcgcgc tcaagacctt ctacgagaac gtgaacctct ccttccagtc
     2461 cgccttcggc gggttcgaca tcgacctcta ctataaagag agcgcgcagt agccgatgct
     2521 cacatttctg gacatcgcgc tcatcgcggt gtattttatc gtcaccgtcg cgatcggcct
     2581 ctggtacgcg cgccgtgccg ggaggagcac cgaggagtac ttcctctccg ggcggaagct
     2641 gccctggtgg atcgccggca ccgcgatggt cgccaccacc ttcgccgccg acaccccgct
     2701 cgcggtgacc gaactggtgc tcacgcacgg gatcgccggc aactggctgt ggtggaacat
     2761 ggtggccggg agcgtgctca cggtcttctt cttcgcgcac ctctggcgcc gctcggccat
     2821 caccaccgac gtcgagttca tcgagctgcg ctatgccggg cgccccgcgg cctttcttcg
     2881 cggcttcaag gcggtgtacc tgggcgtctt catgaattgc atcatcatgg gctgggtgaa
     2941 cgtcgcgctg gccgagatcc tcacgcacat cttcggactc accacgctcc aggtgttcgc
     3001 ggcggtggtc tcgtgcatgc tctgcgtggg ggcgtactcg gcgctctccg gcctctgggg
     3061 ggtggcgatc accgacatgt tccagttcgt agtcgcgatg gccgggtgca tcgtcctggc
     3121 gatcatcgtg ctgtccgtgc cccaggtcgg agggatcgcc gggcttgccg cgtcgcttcc
     3181 gggcgccatg ttctccttcg tgcccgattt cggcctctcg cccgccgcgg cggcctccgg
     3241 cgtgaccgcg ctcgcgggga gcgccttcgc cgcctatgcg ctcatccagt ggtgggcgtc
     3301 gtggtatccc ggatccgagc ccggcggcgg cggctacgtc gcgcaacgca tgatgtcggc
     3361 caaagacgag cggcacgcga tcctggcgac gctctggttc gtcatcgccc actacgccgt
     3421 gcgcccgtgg ccctggatcc tcgtggccct cgcggccgcg gtcctgtatc cgggcagcgg
     3481 caagtcgggc tatatcctgg ccatgcgcga ccacctgccg gcgggcctgc tcggcttcct
     3541 ggtggcagcc ttcctcgcgg cctacatgtc caccatcgcg acccacctga actggggcac
     3601 ctcctatctg atgaacgacc tgtaccgacg ctttatccgc gtcgacgcga gcgagcgcca
     3661 ctatgtccgc gtctcccgcg ccatcacgat cctcctcacg gcggtctcca gctcgctcat
     3721 cttcgtcatc tcgtccatct cgggggcgtg gggcttcgtg atcgagttcg gcgcgggact
     3781 gggcctggtg ctcatcctgc gctggtactg gtggcgcccc aacgcctggt cggagatcac
     3841 cgcgatggtg gcgcccttcg gcggctgggc gatctcccac ttcgcgcttg gcctctcctt
     3901 tcccgtgagc atgtacgtga tcgtccccgt caccaccgcc gcctggctcg gggtgacctt
     3961 cctcaccgca cccaccgagg acgcgcgcct ggtcgccttc tatcggcgcg tgcgcccggc
     4021 cggacccggc tggagcggct tcgcgcggcg ccacggtctc gagccgagcg acggctcgat
     4081 tgcggcgctc gcgatcaact ggctcgtggg cgtggtgatg atctacgccg cgcttttcgc
     4141 atcgggcaat attatcttcg ggaaatacgg cgccgggttc gccctcgcgg ccgtggcggc
     4201 ggcttccttc gccttcctcg ctcggcggat cggcatgaac gaacgcgtta gctgacattt
     4261 tatacgattg ccctggattt tttgtgatta tttttttcct atcatgaaaa attcctcctc
     4321 gcttttttcc gccgcgtgaa tatttttatt gaaagaaata caacctcgga aacgctttcc
     4381 ccataaagac gctggcggtt aacgcgcaac atcgttggac cgccggcatt gaccaatcac
     4441 acgaccacta caccgttagc gccgttcgag gagatgccat gaacgagtac accgcgacga
     4501 tcgccgcctg ctatgcgcgc cattccaatg ccgtgctcga cttcctgtcc cgctttttgc
     4561 gcgaccgtca tgccgcagaa gacctcacgc aggaaacctt tatccgtctg ctgaaccgga
     4621 aggaggcgct ggaccccgac catccaacgg cgctgagcta tctcctgacc atcgcgcgcc
     4681 acatcgcctg cgaccacctg cgccatcagg gcgtggagcg gcgctatcag accatgctgc
     4741 agatggagga ggtcacgctc aacgagcgtt tcttcaacga tgttgcggac gcacatctcg
     4801 agggcgaggt gatctcgacg atgcacgacg cgatcgattc gatgccgccc cgcgcgcggt
     4861 cgctcattct tggcaaattt tatcagcgga aaaagaacaa tcggctcatg cgcgagctgg
     4921 gcatctcgaa cttcatcctg caacggacgc ttcgcgaggc catgacggcg cttcgggaat
     4981 tgttgcgcgc gcactatcgt ccgtcggaac caagtagtaa ttgacagcgc cccccgcccc
     5041 ctgttgcatg ggacgaacgc atctgcaccc atacggccgg agcgactgaa tgagcaccaa
     5101 cagcaggcct tcgatatcgg tcgaggacgc gatcgacatt atcatccgtt cgacggcgca
     5161 cctcgagccg gagaccgtct ccctcgtcga tgccggcggc cgtatcctcg ccgaggacat
     5221 cgtcgcaggc gccgacatcc cggcattcga caactcggcc atggacggat acgccgtgcg
     5281 cgcagccgat atcaccggcg caacgcgcga tcgcccggcg cgactcgccg tacgcggcga
     5341 ggttcgtgcg ggcggcgatc tcctccgtct ggacgatccc gaatcggccg tccggatcat
     5401 gaccggcgca cccattccgc ccggcgcgga cacggtggtc atggtcgagg acacggccga
     5461 gactgacggc gccgtttcga tatacgacgc cgtcccgatg gggtcgaaca tccgccgcgc
     5521 cggcgaggac atcccgcgcg gcgccgtaac cctcacggcg ggtgagcgat tgcgcgcctc
     5581 tgagatcggc gtgctcgcct ccctcaatgc gtcaaccgtc cgcgtggcgc gccgcccgcg
     5641 cgtcgccatc atctccaccg gcgacgaggt gatcgaggtg ggcgcaccgg cgcgcgatgg
     5701 cgttgtgcgc aatagcaatg cctacatcct ccgagaggag gtccgccgca ccggcgggga
     5761 gccaacctat ctgggcatcg cccccgatac gctggaagcc acccgcgacc ttctggcgcg
     5821 cgcatccgag cacgacgtca tcatcaccag cggcggggtg tcgatgggcc ggtatgattt
     5881 cgtccgcgac gcgctcgcgt cgatcggctt cgacatcagc atcgagtcca ttctcatgaa
     5941 gcccggaaag ccctgcgtct tcggtcgccg ggattcgacg ctcttcttcg gcctccccgg
     6001 caatcccgtc tcgacacagg tggcctttct ccaattcgtc cggccggcgc tcctggcgct
     6061 catgggcgcg cgcaaacgcg acaagccgct ggtacgcgcg atcctcgaag agcccatcga
     6121 caaaaagccg ggacgcgtgc acttcatccg cggcgtgttc acgatcgaaa acggggaatt
     6181 ccgcgtgcgg accaccggcc cgcaggggtc ggggattttg cgctcgatga gcagagccaa
     6241 ctgcctcatc atcctccccg ccgacatgcg ccgcgccgaa cgcggcgacc gcgtgaccat
     6301 ccagctcatc gaccacgacg aggtctgatg gcgccgcgaa ccggcatact cctctattcc
     6361 ggcggactcg acagcctgct tgccgcgcgg attctgctgg accaggggat cgaactcatc
     6421 ggactccact tcgtcctccc gttcttttcg ccgcatgccg atcccgcaga cacggccgcg
     6481 gcgcgccagg ccgcgcgcat cggactttcg ctgcggcaca tccgctgcgg gcgcgactac
     6541 atggacatgg tcgaacgccc cgcgcacggg tacgggaagc acatcaatcc gtgcatcgac
     6601 tgtaagatat tttttctgca acgcgcgcgg gaggagatgg agcgcacggg cgcggccttc
     6661 gtcgcgaccg gcgaggtggt cggccagcgg ccgatgtcgc agatgcggca catgctcaac
     6721 cacatcgaac acgcgtcggg actcgacggg tatttattgc gccccttgag cgcgaggctc
     6781 ctgaaaccga cggtcgcaga ggacgaagga atcgtggacc gcgaacgcct gctcgacatc
     6841 agcgggcgat cgcgccgccg gcagatggag ctcgccgcgc gctacgagat cgaggactat
     6901 gcctcgcccg ccggcggctg cctgttcacc gacgccaatt acgagcgccg ccttcgcgac
     6961 cttttcgcgc accggccgga ctacgacctg gccgcggtgt acctgctttc cgtgggccgc
     7021 catttccggc tcaacgagcg cacccggctc atcgttgcgc gaaacgagcg ggaggggagc
     7081 gcgctcgagc gcgctcgcga cagcgccgac tgtctcctcg tcccggactt caagggaccc
     7141 accgccctcc tcacgggaga cgtcgacgag gcgacgctcg ccctggccgg ctcgatcatg
     7201 ctgcgctacg gacgcccgga ggggatcaac gccattcagg tgttccggcg gggggatgat
     7261 ccgcggacca tgacgatcgc gcgccccatc ggcgaggatg agctgaagcg cgccgcgctc
     7321 gcataacgcg accggaacga ccgccgcgga cgacgcgtcg ccgcacgcgg gacgttatgt
     7381 ggcatcaggc cggattttca cgcgtaccgg tttcgccttc cagatttcgt cggcatattc
     7441 cttgatcgtc cggtccgacg agaacttccc gatccgggcg gcgttgagga tcgatttccg
     7501 cacccagccc ggccggtcca gatagtcgcg ggccacgcgg tcctggcaat tcaggtacga
     7561 ctcgaaatcg gccatcagca ggtaatggtc accctgcgcg agcagggaat cgacgatggg
     7621 cgcgaagagc cccggctgcc ccggagagaa ggcgccgccg ccgatcatgt ccaccacccg
     7681 cttcagctcc gggttcctgt gatagtagtc ccacgggttg tagccggtga tccgcgtgct
     7741 caccacctgg tcggccgtga ggccgaagat gtagatgttc tcgctcccga cctcctccat
     7801 gatctcgaca ttggcgccgt cgagcgtgcc cacggtgagc gcgccgttga gcgcgaactt
     7861 catgt
//
LOCUS       DAORHF010000177         7693 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9140_length_7693_cov_4.761456, whole genome shotgun sequence.
ACCESSION   DAORHF010000177 DAORHF010000000
VERSION     DAORHF010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7693
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9140_length_7693_cov_4.761456"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PKJ16_13380"
     CDS             complement(<1..403)
                     /locus_tag="PKJ16_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HNV48029.1"
                     /translation="MRHPREPIASYSHAAGAIFFIAGTAYLVALARGSIMLSLSLVVY
                     GLAAIFLFASSALYHAFKTGENESSVLRKLDHLAIFFMIAGTYTPVCYAYLEGGWFAG
                     IIIAQWGLVLFGLFFKLLYLSAPRHLSTGIYV"
     gene            complement(418..717)
                     /locus_tag="PKJ16_13385"
     CDS             complement(418..717)
                     /locus_tag="PKJ16_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48030.1"
                     /translation="MERQPKLRLKPIDKNDDESMRIYGMTGKTVESFNSYVADFLQTV
                     AILRENIEKAHTTADQEKAAEMREHIEHVERKIDELTEAMQRIADDMSSLVEFEK"
     gene            863..2167
                     /locus_tag="PKJ16_13390"
     CDS             863..2167
                     /locus_tag="PKJ16_13390"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV48031.1"
                     /translation="MKKRNATVVLADTGTDLANAVRRCFEPFGGAGALIKSSGDVYLK
                     VNAVDLKPHCYTDPAVIRETVLLFTAAGARRIYVIENCTQSNFTRMVFAATGIRAVCD
                     ETGAVPVCLDETGATPVFLNTTEQFADISSFVHERLVERRDENLYVSIPKLKTHSMTS
                     VTLSIKNQFGFVHQASRVADHNYRLHMRLADLYRILRPDFALVDGIIATTNGHYIAEG
                     NAGRSVVPMGLLVAGSDPLAVDAVAAGLMGYSVDDVEHLRLCRKTGIGTGDLRRIDII
                     NKRLYAERKKKLTHELLDDFPKDLVILRGKERCCAEGCRRNTETVAEVFHRDHGGRGG
                     FTIIMGKGIPHEQVNAIAGPVHIAGGCAIEEHAHALIARLGKKKVTMSPGCNNLPDTV
                     YALCRHMRVSPLRLVPVNPLSSLALLAGAKARGSRAVIPKLL"
     gene            2179..2307
                     /locus_tag="PKJ16_13395"
     CDS             2179..2307
                     /locus_tag="PKJ16_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48032.1"
                     /translation="MKYSINDTLPDYSFRNIEGNPVRLGDVAGGRTALVVFYRGWW"
     gene            2326..2733
                     /locus_tag="PKJ16_13400"
     CDS             2326..2733
                     /locus_tag="PKJ16_13400"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNV48033.1"
                     /translation="MAELNTHLGEIDGWNARLIAVSVDPPEEALRFKRDLGLAFELLS
                     DPEREAIIDMDILNPRERGGIARPNFYILAPQREIVFHSQDGIARRATFEAVRAFMER
                     YRKDPSHRVFNDARALATPTLKDTLLSIPRKYF"
     gene            2767..3201
                     /locus_tag="PKJ16_13405"
     CDS             2767..3201
                     /locus_tag="PKJ16_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48034.1"
                     /translation="MTHHDEGNYRAKRHGEDAPAAEIMDAVANGARDGKIGCAAAEAI
                     ATRLSVPPDEVGRAIDLAELKISACQLGLFGYGKGVKLIKPAESVAEDLAGEIRAAAT
                     DGRIACTDVWAIADRRGMTRLDAACACDALGLKIAACQLGAF"
     gene            3578..4975
                     /locus_tag="PKJ16_13410"
     CDS             3578..4975
                     /locus_tag="PKJ16_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48035.1"
                     /translation="MKVTILIVEDDAVIAHDFQRSLAVRGHAVAGILRTGEDAVAFCE
                     TARPDLIIMDIGLAGAMDGITAAERILEKHDVPVLYVSANSDESTFARAMETGPWGFL
                     VKPLQDASITTTIDIILKRLEAELNLKRSEEKFRRFFNDDLTGDFIAAPDGTILDCNP
                     AFLKLYGIPSPDDATGASITRFFADRTEWRGLVERLHENRILENYETASIRENGERIH
                     VVVNLSADIDARGGITEIKGYLYDITRRTTMENELRAALAEREVLLKEIHHRVKNNFQ
                     VTSSLINLQAKHIADRDARDKLDHIRNQLHSMALAHTIAYESSRPSSPNAGTLIRQIS
                     ENHSGACSRRSIDISISVDDEHHLSLDQAIPCALIINELIENAATHAFIGRETGAIHI
                     SLTAEDEDYLLIVEDNGIGMPGPASESFDAGFGMQLVVLIVNQLGGCLRITSPREGGA
                     RFEIRFPIAPALTPP"
     gene            complement(5278..6162)
                     /gene="htpX"
                     /locus_tag="PKJ16_13415"
     CDS             complement(5278..6162)
                     /gene="htpX"
                     /locus_tag="PKJ16_13415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HNV48036.1"
                     /translation="MATFKRVFFFIIVNVLVMATISIILSVLGVGRYVDAYGLNIPKL
                     VAFCLVWGMSGAFISLLISRWMVKRMMGIQLIDPNAASGAEREVYEMVRRLSTAAGIP
                     MPEVGIYDSPELNAFATGPTKKRSLVAVSTGLLEAMNRSEVEGVIGHEVSHIANGDMV
                     TMTLIQGVVNAFAMFLSRVISFAVSRFVNEDLEFIVRFVLTLVLDILFTILGSIVVCY
                     FSRIREYKADRDSAKIAGRDKMIAALRRLQANMDAPVDNRGDAIASFKISGKKKFLLF
                     ATHPPLEDRIAALENANL"
     gene            6363..7091
                     /gene="cas6"
                     /locus_tag="PKJ16_13420"
     CDS             6363..7091
                     /gene="cas6"
                     /locus_tag="PKJ16_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HNV48037.1"
                     /translation="MRTLIELHSDRTIVLPVGYNALIRELLRSRLSGTANGVGDDDTA
                     FAFSLSLSKGAFDRTLREFTYRDRLRFFASSPDARLAERLATSLEREPRVRLGANELR
                     VASATMIPRATIESGMLRVRALTPCTAYGPITLHGGITQTCFFTPFEEEFAQLTNENL
                     ARLWRAINKTECPHELGIRPLFHGTGTRRSVHYGAGEDRVTLTAWWGMYELTGEPAFL
                     DFALDGGIGMDTSKGFGFVEATPS"
     gene            7088..7585
                     /locus_tag="PKJ16_13425"
     CDS             7088..7585
                     /locus_tag="PKJ16_13425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HNV48038.1"
                     /translation="MIRKCATDDFETMYEIINDAARAYKGVIPDDRWHEPYMPREELR
                     REIDDGVAFWGYEFDGVLSGVMGIQDVKDVTLIRHAYVRTARRGSGIGSALLAHLRTL
                     TDRPILIGTWTDAAWAVRFYERHGFIATSREETARLLRAYWNIPERQVETSVVLKPPP
                     GLPLR"
BASE COUNT         1527 a         2503 c         2359 g         1304 t
ORIGIN      
        1 gcacgtagat gcccgtcgag agatgccgcg gcgccgagag gtacaacaac ttgaagaaca
       61 gcccgaagag caccagcccc cactgcgcga tgatgatccc cgcgaaccag ccgccctcaa
      121 ggtaggcgta gcagaccggc gtataggtgc cggcgatcat gaaaaagatc gcgaggtggt
      181 cgagctttcg aaggaccgac gattcattct cgcccgtctt gaaggcgtga tagagcgcgc
      241 tggacgcgaa gaggaaaatt gccgcgaggc catatacgac aagcgagagc gacagcatga
      301 tcgagccgcg cgcgagggcg acaaggtatg ccgtacccgc gatgaagaat atcgcgccgg
      361 ccgcgtgcga ataacttgcg atgggttccc ggggatgtcg catggtgatg cgccgcccta
      421 cttctcgaac tcgaccagcg aggacatgtc gtcggcgatg cgctgcatgg cctcggtgag
      481 ctcgtcgatc ttgcgctcca cgtgctcgat gtgctcgcgc atctcggcgg ccttttcctg
      541 gtcggccgtc gtgtgcgcct tctcgatgtt ctcccgcaga atggccaccg tctggagaaa
      601 atccgccacg tagctgttga agctttcgac cgttttcccg gtcatgccgt atatccgcat
      661 ggactcgtcg tcgttcttgt cgatgggctt gagcctgagt ttcggttgcc gttccatccc
      721 gtcctcccgc gatgacgata ccgtaccgcg cgggggcgtc gagcgtcaac cgataaatcg
      781 cttgcgcaaa cggtccgccc gtgccataca gaacaaaacc gagaacgacg ggcatttcgc
      841 ccgcatcacg gggaggttgt cgatgaaaaa acgaaacgcg accgtcgttc tcgccgacac
      901 cggcacggat cttgccaatg ccgtccggcg gtgcttcgag cccttcggcg gagcgggcgc
      961 cctcatcaag tccagcggcg acgtgtacct caaggtgaac gcggtggacc tcaagccgca
     1021 ctgctacacc gatccagcgg tgatccgcga gacggtcctc ctgttcacgg ccgcgggggc
     1081 ccggaggatc tacgtgatcg agaactgcac gcagtcgaac ttcacgcgca tggtcttcgc
     1141 cgcgaccggc atacgcgccg tgtgcgacga gaccggcgcc gtccccgtgt gcctcgacga
     1201 gacgggcgcg acgcccgtgt tcctgaacac gaccgagcag ttcgccgaca tctcgtcatt
     1261 cgtgcacgag cggctcgtgg agcgccgcga cgagaacctg tacgtctcga tcccgaagct
     1321 taagacccat tcgatgacct cggtcacgct gtcgatcaaa aaccagttcg gcttcgtgca
     1381 ccaggcgagc cgcgtcgcgg accacaacta ccggctgcac atgcgcctcg ccgatctcta
     1441 ccggatactg cggccggact tcgcgctcgt ggacggcatc atcgcgacca ccaacgggca
     1501 ctacatcgcc gagggaaacg ccggccgctc cgtggtgccg atgggactcc tcgtcgccgg
     1561 ctccgacccc ctcgcggtgg acgccgtggc cgcggggctg atggggtatt ccgtggacga
     1621 tgtggagcac ctgcgcctgt gccggaagac cggcatcggg accggcgacc tgcgccgcat
     1681 cgacatcatt aacaaacgcc tgtacgcgga gcggaagaag aaactcacgc acgagctcct
     1741 cgacgatttc ccgaaagacc tcgtcatcct ccgcgggaag gaacgctgct gcgcggaggg
     1801 ctgccgacgc aatacggaaa ccgtggccga ggtgttccac cgcgaccacg gcggacgggg
     1861 cgggttcacc atcatcatgg gcaaggggat cccgcacgag caggtgaacg cgatcgcggg
     1921 gcccgtgcac atcgccggag gctgcgcgat cgaggagcac gcgcacgccc tcatcgcgcg
     1981 cctgggaaag aagaaggtga cgatgagccc cggctgcaac aacctgcccg acacggtgta
     2041 cgcgctctgc cggcacatgc gcgtgagccc cttgcggctg gtgcccgtga acccgctctc
     2101 gtcgctggcg cttttagccg gggcgaaggc gcgcggatca cgggccgtga taccgaaact
     2161 gctctagcgg aggggaccat gaaatactcc atcaacgaca cgctgcccga ctattcgttc
     2221 cgaaacatcg agggcaaccc ggttcggctg ggtgatgtcg ccggcggccg caccgcgctc
     2281 gtagtgttct accggggctg gtggtgaccg ctgtgccgca gccagttggc tgaattgaac
     2341 acgcacctcg gggagatcga cggctggaac gcgcgcctga tcgccgtgtc ggtggacccg
     2401 cctgaggagg ccctgcgctt caagcgcgac ctgggcctcg ccttcgagct cctgtcggac
     2461 ccggagcgcg aggcgatcat cgacatggac atcctgaacc cgagggagcg cggcgggatc
     2521 gcgcggccca acttctacat cctcgcgccg cagcgggaga tcgtgttcca ctcgcaggac
     2581 gggatcgccc gacgcgcgac cttcgaggcc gtgcgcgcct tcatggagcg ctaccggaag
     2641 gacccgtcgc accgggtgtt caacgatgcg cgggcgctcg cgacgcccac gctcaaggac
     2701 acgctgctct cgataccgcg gaaatatttc taagcgcatg caccaatcga cgacacggag
     2761 gacgccatga cccatcacga cgagggaaac taccgggcga agcggcacgg cgaggacgcg
     2821 cccgccgccg aaatcatgga tgccgtcgcg aacggcgctc gcgacggaaa gatcggctgc
     2881 gccgctgcgg aggcgatcgc gacgcgattg tccgtcccgc ccgatgaggt gggacgcgcg
     2941 atcgaccttg cggagctgaa gatctccgcg tgccagctcg gactcttcgg ctacgggaag
     3001 ggcgtgaagc tcatcaagcc ggcggaatcg gtcgcggagg acctggcggg cgagatacgc
     3061 gcggccgcga ccgacgggcg catcgcctgc acggacgtct gggcgatcgc cgaccgccgc
     3121 ggcatgacgc ggctcgacgc ggcgtgcgcc tgcgatgcgc tgggattgaa aatcgcagca
     3181 tgccagctgg gggcgttctg acgagaaaat ccccgccgtc gtcgctgcgc tcgttacgat
     3241 gattgccgat taacccgcac ccaaatattc cgatgcatta atcagtggcg atgaaatcgg
     3301 atcgattatt ttagcgcttc cgaaaaagaa tcgcttgttt tccccaccat tcggtcgcta
     3361 cacttgacgt gcaccatgct cccgatcgtc cgtgcgaatc gcacagtcgc aggggcggca
     3421 tgcgggtttg cgccgcatgc gggatcgtgc tgacgaggtg cgccgcgacg gagacggcgc
     3481 gccgagcgtt cactctcgtc gaatcggcga cgggcggaga tgcgattccg ctatcgccac
     3541 tgaaacagaa accattctcc gagcggaata gcgcatcatg aaagtaacca tactcatcgt
     3601 cgaggacgac gcggttatcg cgcatgattt tcagcggtcg ctcgccgtgc gcggccacgc
     3661 cgtcgccggc atcctgcgca ccggcgaaga cgccgtcgcc ttttgcgaaa cggcgcggcc
     3721 cgacctcatc atcatggaca taggcctggc cggcgcgatg gacggcatca ccgcggcgga
     3781 acgcatcttg gagaagcatg acgtgccggt gctctacgtg tccgccaact cggacgaaag
     3841 caccttcgcg cgggccatgg agaccggccc ctggggcttt ctcgtcaaac cgctgcagga
     3901 tgcgtcgatc accacgacca tcgacatcat cctgaaacgc ctcgaagcgg aattgaacct
     3961 caaacggagc gaggaaaagt tccggcgctt cttcaatgac gacctcacgg gagactttat
     4021 cgccgcaccc gacgggacca tcctcgactg caacccggcc ttcctgaagc tctacggcat
     4081 tccgagtccg gacgatgcga ccggcgcatc aatcacgcgg ttcttcgcgg accgcaccga
     4141 atggcggggc cttgtcgagc gcctgcacga aaaccggata cttgaaaact acgagaccgc
     4201 gtcaatccgg gagaacggcg aacggataca tgtcgtcgtg aacctctcgg ccgacatcga
     4261 cgcccgcggc ggcatcaccg agatcaaggg ctatctctac gacatcacgc ggcgcacgac
     4321 catggagaac gaactccggg cggcgctcgc ggagcgggag gtcctcctca aggaaatcca
     4381 ccaccgcgtg aaaaacaact tccaggtgac ctccagcctc atcaaccttc aagcgaaaca
     4441 catcgccgac cgcgacgctc gggacaagct ggatcatatc cgcaaccagc tccattcgat
     4501 ggcgctggct cacacgatcg catacgagtc gagccggccg tcatcgccga acgccgggac
     4561 cctcatccgg cagatctcgg agaaccacag cggcgcctgc tcgcgtcgct ccatcgacat
     4621 ctcgatctcc gtcgacgacg agcaccacct cagcctcgac caggcgattc cctgcgccct
     4681 catcatcaac gagctcattg aaaacgcggc aacccacgcc tttatcggac gcgagaccgg
     4741 cgctattcac atctcactca ccgcggagga cgaggactat ctgctgatcg tcgaagacaa
     4801 cggtatcggc atgccgggac ccgcatcgga atcgttcgac gctggtttcg gcatgcaact
     4861 cgtggtcttg atagtaaacc agctcggagg ctgcctgcgc attacatcac cccgggaggg
     4921 tggagcgcgt ttcgagatcc gctttccgat cgcaccggct ctgaccccgc cctgacgaac
     4981 agtcgcacac gaacgagatc ccatcgacac cgcgccgttt ttgcacacaa aaaataattt
     5041 tgttcaggag aactgcgcca ccaagagtta cgagctcagg cctggatgag catggaccga
     5101 gctaagaggc acctaagagg tacctaagag gtacctaaga gggagcttgg aggtagaata
     5161 gaggtgaaat tgagcggaat ggcatggtaa ttggctgcat gatgcaataa ttcgacaaaa
     5221 ggccggaaag catcaccttc cggccttttg gctctacttg acctgacgag aaacagccta
     5281 caggttcgca ttctccaggg ccgcgatccg gtcctcgagg ggcggatgcg tggcgaagag
     5341 caggaacttc tttttgccgg agatcttgaa cgatgcgatg gcgtcgcccc ggttgtccac
     5401 cggcgcgtcc atattcgcct gcaaccggcg cagcgcggcg atcatcttgt cgcgaccggc
     5461 gatcttcgcg ctgtcgcgat cggccttgta ttcgcggatg cgcgaaaagt agcacaccac
     5521 gatgctgccc aggatggtga acagaatgtc gagcaccagc gtgagcacaa accgcacgat
     5581 gaactccagg tcctcgttca cgaaccggct cacggcgaag ctgatcacgc gcgagaggaa
     5641 catcgcgaag gcgttcacca cgccctggat gagcgtcatg gtgaccatgt cgccgttggc
     5701 gatgtgcgac acctcgtggc cgatcacccc ctccacctcg gagcggttca tcgcctccag
     5761 gaggccggtg gacacggcca cgagcgagcg cttcttggtg ggcccggtcg cgaaggcgtt
     5821 gagctcgggc gaatcgtaga tgcccacctc cggcatgggg atgccggccg cggtggagag
     5881 ccgccgcacc atttcgtaca cctcgcgctc ggcgccgctt gcggcgttcg gatcgatgag
     5941 ctggatgccc atcatgcgct tgaccatcca gcgcgagatg agcagcgaga tgaacgcgcc
     6001 gctcatgccc cacaccaggc agaaggcgac gagcttcggg atgttgaggc cgtacgcgtc
     6061 cacgtaccgc cccaccccga gcacggagag gatgatggag atcgtcgcca tcaccaggac
     6121 gttgacgatt ataaaaaaga atacccgttt gaatgttgcc atagattctc ctcgttgtca
     6181 tgcggcaccg gtatccggaa acgcgcccgc gcgactggtt acaaaaaaat cgcggttccg
     6241 ccggaaatcc cggattacgg aaaaagatac acccgcccat tccgttcgtc aatattttta
     6301 tctcgcggag atgcggcgga acgcgcttga cagaccggcc ggactggaac aggaaaactt
     6361 ccatgcgcac tcttatcgag ctccattcgg accggaccat cgtcctgccc gtcgggtaca
     6421 atgccctcat ccgcgagctg ctccgctcgc gcctctccgg tacggccaac ggcgtggggg
     6481 atgatgatac cgccttcgca ttctcgcttt cgctttcgaa gggcgcattc gaccgcacgc
     6541 tgcgcgaatt cacctaccgg gaccggctgc ggtttttcgc atcatcgccc gatgcgcgcc
     6601 tcgcggaacg cctcgccacc tcgctcgagc gagagccgcg cgtgcggctc ggcgcgaacg
     6661 agcttcgcgt cgcctccgcc acgatgatcc cgcgcgcgac catcgaaagc ggcatgctgc
     6721 gcgttcgcgc cctgacgccc tgtacggcct acggtcccat caccctgcac ggcggcatta
     6781 cgcaaacctg cttcttcacg ccattcgaag aagagttcgc ccaactgacg aacgagaacc
     6841 tcgcgcggct ctggcgcgcc atcaacaaaa cagaatgtcc gcatgagctc ggcatacgac
     6901 cgctctttca cggcacgggc acgcgccgaa gcgtccacta cggcgccggc gaggaccggg
     6961 ttaccctcac ggcctggtgg ggcatgtacg aactcacggg cgagccggcc ttcctcgatt
     7021 tcgcgcttga cggcggcatt ggcatggaca catcgaaggg cttcgggttt gtggaggcga
     7081 cgccatcatg atacggaaat gcgcgaccga cgatttcgag acgatgtacg agatcatcaa
     7141 cgacgcggcg cgggcataca agggcgtgat cccggacgac cgctggcatg agccctacat
     7201 gccgcgcgag gagttgcgcc gcgaaatcga cgatggcgtg gcgttctggg gatatgaatt
     7261 cgacggggtt ctctcggggg tgatgggcat ccaggacgtg aaggacgtca cgctcatccg
     7321 ccacgcctac gtgcgcaccg cccggcgcgg ctccggcatc gggagcgcgc tcctggcgca
     7381 cctgcgcacg ctcaccgacc ggcccattct catcggcacc tggaccgatg cggcgtgggc
     7441 cgtgcgcttt tacgagcggc acggcttcat cgcgacctcg cgcgaggaga cggcgcgatt
     7501 actgcgcgcc tactggaaca tccccgagcg gcaggtcgag acgtcggtgg tgctcaaacc
     7561 acctcccggc cttccccttc gctagacgtc ggtggcgcca aaccccctcc cgacctcccc
     7621 cttcgccagg gggaggggtg ggcttgcccc gattttgcgg acacgcagtt aagcaacatt
     7681 cttaatccag ccg
//
LOCUS       DAORHF010000178         7661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9187_length_7661_cov_4.167631, whole genome shotgun sequence.
ACCESSION   DAORHF010000178 DAORHF010000000
VERSION     DAORHF010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9187_length_7661_cov_4.167631"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PKJ16_13430"
     CDS             complement(<1..562)
                     /locus_tag="PKJ16_13430"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF025065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HNV48039.1"
                     /translation="MKTITVPFGERAYRVTIGCGALESGDWAHFVDRRERCAVIVSQR
                     VLELHGAYIERVLAGLESADLLPMPDGEERKSYAHAERYFNELLSRGYGRGATVIAIG
                     GGVVGDFAGFVAAAYMRGVRFIQVPTTLLAMVDASIGGKVAVNISAGKNIVGAFHQPA
                     AVVADIAFIETLPDEELKNGIAESLKH"
     gene            complement(559..1017)
                     /locus_tag="PKJ16_13435"
     CDS             complement(559..1017)
                     /locus_tag="PKJ16_13435"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV48040.1"
                     /translation="MKFRMGGAVRVRVSALIVEDGRVLLIAHKKKDDIYWLLPGGGVG
                     FGETLPQALEREMKEELGISLEVGELLLVSDSINPDARRHIVNVVFRGVRRNGDLHLG
                     NDRRLYTYRFFGLDELDDIIIFPPLRDEIGSILSDSFSCARYQATQWLPL"
     gene            complement(1014..1772)
                     /gene="rnc"
                     /locus_tag="PKJ16_13440"
     CDS             complement(1014..1772)
                     /gene="rnc"
                     /locus_tag="PKJ16_13440"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HNV48041.1"
                     /translation="MDDDENSTSDHHRKSKRSRYRQLEHLQSVLKLRFRNKSLLGKAL
                     THRSYVNECGAIAKDNERLEYLGDSVLALVVNEYLFKKFEDYLEGDLAKIKSAVVSEA
                     TLAAVARELNLGSYIQMGRGEEMSGGRDRTSILANTVEALIGAVYLDAGLRESRRFVL
                     GLLKGAIDRIDRLSYYRDPKTTLQEIVQKKYKDRPEYRVIEESGPDHNKNFLVQLVVQ
                     GRDICVGTGSSKRKAEIEAALHALEIIHRGDAPL"
     gene            complement(1811..2047)
                     /gene="acpP"
                     /locus_tag="PKJ16_13445"
     CDS             complement(1811..2047)
                     /gene="acpP"
                     /locus_tag="PKJ16_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011230691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HNV48042.1"
                     /translation="MAVDFERIKKLIVDQLGVDESEVTLEASFVDDLGADSLDTVELV
                     MTLEEEFGIEISDEEAEKIVTVGDAINYINSKLK"
     gene            complement(2223..2420)
                     /gene="rpmF"
                     /locus_tag="PKJ16_13450"
     CDS             complement(2223..2420)
                     /gene="rpmF"
                     /locus_tag="PKJ16_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HNV48043.1"
                     /translation="MAVPKRRKSKSKSRMRRSHNALVAPNLRPCTRCGAYVMPHRVCP
                     ECGHYKGTLVLEPKVKVEKTK"
     gene            complement(2528..3244)
                     /locus_tag="PKJ16_13455"
     CDS             complement(2528..3244)
                     /locus_tag="PKJ16_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48044.1"
                     /translation="MPQREIEIQRNPIERVLMAAKDIIRKNRRGFLYGLIGVIAAAGV
                     AIGLAVYFSHRAGVELAKFEAVMEEYRATAALDPDTRAAKAGEIAEKLKAIIDGSWFG
                     YVNSYGKYYLGAVYFENGRYAEAKECYRAFGSKSSSEIFAPLALQQAARCAEMQGNLK
                     EALEIGASIEKRFSESELADQILYDIARLNQRNGDIFKAREYYNKIIISFPQSLYAKK
                     AKDSLFLLGYYEGRKKTGAQ"
     gene            complement(3300..5657)
                     /gene="cmk"
                     /locus_tag="PKJ16_13460"
     CDS             complement(3300..5657)
                     /gene="cmk"
                     /locus_tag="PKJ16_13460"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HNV48045.1"
                     /translation="MGSDHIIVAIDGPAGSGKSSVSKEVAKRLGISYVDSGAIYRAIT
                     WLILRNRGAVERGAGFADDLAGASIDQRFLPDGACRTTVDGVDVSDEIRTETIAKNIG
                     IISDDVRVRAFVNDLLRERGRRESLIMDGRDIGTVVFPDADVKIYLDASVAIRAERRI
                     NEYREKGKNLDVNEITKQIIQRDSEDTGRPVGRLVQADDAVYLDTTALTKDEVIDRIV
                     EIITQHSGTQMEHSTNGHDDAISMEYIADSLLDGVAPNTIVEGEIVTIDGDFAYVNVG
                     TKYEGKVRLNEFDAPPVVGDRINVMLVNRKLFDGTFVFSKRAADQEVAWKRFMEHCRS
                     GAQTVSGTIKGTTKNGLFVDVFGAQAYLPFSHAADLKAKKSQEVRIPYEFVIKSVDEK
                     KRGIVLSRKDYLDEAFAKKWEEFVAAHAVGDRIRGRGVRFVESGVIVDCDGISAFLHR
                     DDLSWRKVIKKKKYCTLGEEAEFMIMGIDQEARRITLGLKQLTEDPWARIDERYKPGD
                     KVPGRVVTVAAFGLFVEIEEGVEGLVNNANLSWTKKNVPAKDAYQKGQKIEVMILDIN
                     KADRRLSLGLKQLLPNPWETVAERFPVGTVLRRPVKKIVSFGMFVELEEDVDGMVHVS
                     DITWDEGNKNPLSSFSVGDEIEFKILEINSTDMKISCGMKQLTKSPWEAIKEKYPPRT
                     RVSGAVSGIVSFGLFVKLEDGVEGLVHVSEVSKKKIENLAEQFKVGDPVGAVVLDVDV
                     NRRRLSLSMKLYDIVTEKEDLDKVLSNTTPKTVTIGDLLKKKLGE"
     gene            complement(5778..6794)
                     /gene="aroF"
                     /locus_tag="PKJ16_13465"
     CDS             complement(5778..6794)
                     /gene="aroF"
                     /locus_tag="PKJ16_13465"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNV48046.1"
                     /translation="MLIVLKPGVTQDEIDHIVEKIKSLKLNPHVSTGVQRTIITVIGD
                     EDIIREQPLEALAGVESVKSILRPFKLVSKETHPDRTVIKVGDVEIGGPRVVVMAGPC
                     AVEGEAAVVEHARMIKKAGAKMFRAGAFKPRTSPYSFQGYGREGVMFMVAARQETGLP
                     FVTEVMDPRDVEFLAEHADMMQIGTRNMQNYNLLKEVGRMRTPVLLKRGMNATINEFL
                     MSAEYILAQGNRNVILCPRGIRTFEDITRNTLDAGAVPVVKELSHLPVICDPSHAMGI
                     RDYVIPAGLAYIAAGADGLIVEAHPNPEDATSDGQQTVNYEQLTDLIARVNKVAKAIG
                     RDCY"
     gene            complement(6870..>7661)
                     /gene="pheA"
                     /locus_tag="PKJ16_13470"
     CDS             complement(6870..>7661)
                     /gene="pheA"
                     /locus_tag="PKJ16_13470"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HNV48047.1"
                     /translation="GPEGTFTQIALLEVFGESVEAVPQKTIPDVFLEVEMDKAPFGIV
                     PVENSTEGAVTYTLDELLDTDLKIVCEKFLRISYCLVSLCPDIRGVKKLYVHPQAAAQ
                     CKSWIRKNLPGAEMHNVASNSLAAETASWDKNSAAISSEISARIYSLNVLDRNIEDSK
                     NNYTRFLVLGKKDNPPTGNDKTSIVCAVKDKPGALYDLLRAFHEAGINMTKIESRPDK
                     KSMWEYNFFIDFNGHTNDTVVRAAVDIMKQETLFFKILGSYPAAN"
BASE COUNT         1373 a         2452 c         2207 g         1629 t
ORIGIN      
        1 cgtgcttgag cgattcggcg atcccgttct tcagctcctc gtccggcagg gtctcgatga
       61 acgcgatatc ggcgacgacg gcggccggct gatgaaaagc gccgacgata ttctttcccg
      121 ccgatatgtt cacggcgacc ttgccgccga tgctcgcatc aaccatcgcc aggagcgtcg
      181 tcggcacctg gatgaaccgg acgccgcgca tgtaggcggc ggccacaaac cctgcgaaat
      241 cgccgaccac gccgcccccg atcgcgatca cggtcgcccc gcgcccgtat ccccgcgaga
      301 gcagttcgtt gaaataccgc tccgcatgcg cgtacgattt ccgctcctcg ccgtcgggca
      361 tgggaagcag gtccgcggac tcaagcccgg caagaacccg ttcgatgtac gcgccatgaa
      421 gttcgagcac gcgttggctc acgatcaccg cgcagcgctc ccggcgatcc acgaagtgcg
      481 cccaatcgcc gctctccaac gcgccacaac cgattgtgac gcggtatgcg cgctcgccga
      541 agggtaccgt gatcgtcttc ataggggcag ccactgcgtg gcctgatagc gcgcgcacga
      601 aaaggaatcg ctgaggatcg agccgatctc gtcgcgaagc ggcggaaaga tgatgatgtc
      661 gtcgagctcg tcgagaccga aaaagcggta ggtgtacagg cgacggtcgt ttccgaggtg
      721 caagtccccg ttgcggcgga ccccgcgaaa caccacgttc acgatgtgtc gccgcgcgtc
      781 ggggttgatg gagtcggaca ccaaaaggag ctccccgacc tcgagcgaga tgcccagctc
      841 ctccttcatt tcccgctcga gcgcctgcgg cagggtctca ccgaagccca cgccgccgcc
      901 cggaaggagc cagtagatgt cgtctttttt cttgtgggcg atcaggagga cgcggccgtc
      961 ttcgacgata agcgcggaga cccgcacgcg tacggcgccc cccatgcgga acttcatagc
     1021 ggcgcgtctc cccgatggat gatctccagc gcgtgcagcg cggcctcgat ctcggccttg
     1081 cgcttgctgc tcccggtccc cacgcagatg tcccgcccct ggaccacgag ctggaccagg
     1141 aagttcttgt tatgatccgg gccgctctcc tcgatgacgc ggtattcggg acggtccttg
     1201 tactttttct ggacgatctc ctggagggtc gtcttgggat cgcgatagta cgagaggcgg
     1261 tcgatgcggt cgatggcacc cttgagcaat ccaaggacga accgcctgct ctccctgagc
     1321 cccgcatcga gatacacggc gccgatcagc gcttcgaccg tgttggcgag aatcgacgtg
     1381 cggtcgcgtc cgccgctcat ctcctcgccg cgccccatct ggatgtagct gccgaggtta
     1441 agctcgcggg cgaccgcagc gagggtcgcc tccgacacca ccgcggactt gatcttggcc
     1501 aggtcgcctt cgagatagtc ttcgaatttt ttaaagaggt attcgtttac gacaagggcg
     1561 agcacggaat ccccgaggta ttcgagccgt tcattgtctt tggcgatcgc cccgcattcg
     1621 ttgacatatg aacgatgggt aagcgctttg ccaagcaggc tcttgttcct gaaccgaagc
     1681 ttaagcacgg actggaggtg ctcaagctgc cggtatctgc tccgtttcga tttccggtga
     1741 tggtcgctgg tgctgttttc gtcatcatcc attatcgtgg gatacttcca ccggtgcggg
     1801 acgcaccgga ctacttgagc ttactgttga tatagttgat cgcgtcgccc accgtcacga
     1861 tcttttccgc ttcctcgtcg gatatctcga tgccaaactc ctcctcgagc gtcataacga
     1921 gctcgacggt gtccagcgaa tcggccccca gatcgtccac aaacgacgcc tcgagagtca
     1981 cttccgactc gtccacgccg agttgatcca cgatgagctt ctttattctt tcaaaatcaa
     2041 ctgccattgc ggtacgtcct ccttctcgtt attcttcaca caccataacg ccggcgctac
     2101 ctccgcaccc ggcgcgtacg ccgccgggga aacggataac aacagacctc tctattcgat
     2161 ggcacctata aaacggttta cgctccttgt caagccatta tacccgcccc ggaaccgggg
     2221 gcttacttcg tcttttccac cttcaccttc ggctcaagga caagcgtgcc cttgtaatgg
     2281 ccgcactccg gacacacccg gtgtggcatc acatacgcgc cgcaccgcgt gcaggggcga
     2341 aggttgggcg ccacaagcgc gttatgcgac cgccgcatgc gcgatttgga tttcgatttt
     2401 cttctcttag gaacagccac ggtgattctc ctctcgtatg gtatccgacc gcttggcgcg
     2461 cgcgtcccgc aggctcgcga ctagcggtgt acctacaatt ttcgcaaagt tattgtcaag
     2521 accaatttta ctgggccccg gtttttttcc gcccctcgta atatccaagc aaaaacagcg
     2581 agtctttggc ctttttcgca tacaacgact ggggaaagct gatgatgatt ttattatagt
     2641 attcccgcgc cttgaagatg tccccgttgc gctggttcag ccgggcgata tcgtacaaaa
     2701 tctgatccgc aagctcgctc tcggaaaatc gcttctcgat gctcgcgccg atctcgagcg
     2761 cttccttgag gttgccctgc atctcggcgc atcgggccgc ctgctgcaat gccagcggcg
     2821 cgaatatctc cgacgacgat ttcgatccaa aggcgcggta gcactccttc gcctcggcat
     2881 aacgcccgtt ctcgaagtac acggcgccca gataatactt cccatacgaa ttcacatacc
     2941 caaaccacga gccgtcgatg atcgccttga gtttttcggc gatttcaccg gccttggcgg
     3001 cgcgcgtatc cggatcgagc gcggcggtcg cccggtactc ctccatcacc gcttcgaact
     3061 ttgcaagctc aacaccggcc cggtgggaaa aatacacggc aaggccgatc gcgacgccgg
     3121 ccgccgcgat aacgccgatc aggccgtaca ggaagccgcg ccggtttttt ctgattatat
     3181 ccttcgccgc catgagcacc cgctcgatgg gattgcgttg gatttcaatt tcgcgctgtg
     3241 gcatcatgga tgatcactcc gtatcatttc gttcgtggcg tccgcgtttc cggcgttggc
     3301 tattcgccca gcttcttctt caggaggtcg ccaatggtga ccgtcttggg cgtggtgttc
     3361 gagagcacct tgtcgaggtc ctctttctcg gtcacgatat cgtacagctt catgctcagg
     3421 gagaggcgtc gccggttcac gtccacatcg agcaccaccg cgcccaccgg atcgccgacc
     3481 ttgaactgct cggcgagatt ctcgatcttc ttcttggaaa cctcggacac gtgcaccagt
     3541 ccctccacgc cgtcctcgag cttcacgaac agcccaaagc tcacgatgcc cgaaaccgcg
     3601 cccgagacgc gcgtccgcgg cggatacttc tccttgatcg cctcccacgg cgatttcgtc
     3661 aactgcttca tcccgcagga tatcttcatg tcggtgctgt tgatctcgag gatcttaaac
     3721 tcgatctcgt cacccacgga gaacgacgac agcgggttct tgttgccctc gtcccaggtg
     3781 atgtcggaga catgcaccat cccgtcgacg tcctcttcga gctccacgaa catgccgaaa
     3841 ctcacgatct ttttaaccgg acggcgcagg accgtcccca cggggaagcg ctccgcaacg
     3901 gtctcccatg gattgggaag cagctgtttg aggcccagcg agaggcgtcg gtcggccttg
     3961 tttatgtcga ggatcatcac ctcgatcttc tgtcccttct ggtaggcgtc cttcgccgga
     4021 acgttcttct tcgtccacga gaggttcgca ttgttcacca gcccctcgac gccctcctcg
     4081 atctccacga acagcccgaa tgcggcgacg gtgaccacgc gccccgggac cttgtcgccc
     4141 ggtttgtagc gctcgtcgat gcgggcccag gggtcctccg tgagctgctt gaggcccagg
     4201 gtgatgcgcc gcgcctcctg gtcgatcccc atgatcatga actcggcctc ctcgccgagc
     4261 gtgcagtatt tcttcttctt aatgaccttg cgccacgaga gatcgtcgcg atgcaggaag
     4321 gcgctgatgc cgtcgcaatc gacgataacg ccggactcga cgaacctgac gccgcggccg
     4381 cggattcggt cgcccacggc gtgggcggcg acaaactcct cccacttctt cgcgaaggcc
     4441 tcgtcgagat agtccttgcg ggacaacacg atgccgcgct tcttttcgtc cacgctcttg
     4501 atcacgaact cgtaggggat gcgaacctcc tgcgacttct tggccttcag gtcggccgcg
     4561 tgcgaaaagg gaagataggc ctgcgcgccg aacacatcga caaacagtcc gttcttcgtg
     4621 gtccccttga tcgtgcccga tacggtctgc gcgcccgagc gacagtgctc catgaaccgc
     4681 ttccaggcga cctcctggtc ggcggcgcgc ttcgagaaaa cgaaggtccc atcgaacagc
     4741 tttcggttta ccagcatcac attgattcga tcgcccacga ccgggggcgc atcgaactcg
     4801 ttcaggcgga ccttgccctc gtacttggtc cccacgttca cgtaggcgaa atcgccgtcg
     4861 atggtcacga tctcgccctc aacgatggtg ttgggggcca cgccgtcgag cagggaatcg
     4921 gcaatatact ccatgctgat ggcgtcgtca tggccgttgg tcgaatgttc catctgagta
     4981 cccgagtgct gcgtgatgat ttccacgatg cggtcgataa cttcgtcctt ggtcagtgcg
     5041 gtcgtgtcga ggtacaccgc gtcgtccgcc tgcaccagcc gccccacggg ccggccggtg
     5101 tcctccgaat cgcgctggat aatctgtttg gtgatctcat ttacgtcaag atttttccct
     5161 ttttcgcggt attcgttgat ccgcctttcg gcgcgtatcg cgaccgaggc gtcgaggtag
     5221 atcttcacgt cggcatcggg aaacaccacc gtcccgatgt cccgaccgtc catgatgagg
     5281 ctctcgcgac gtccgcgctc gcgcaggaga tcgttcacaa acgcacggac gcgcacgtcg
     5341 tcggagatga taccgatgtt ttttgcgatg gtctcggtgc ggatctcgtc ggagacgtcg
     5401 acgccgtcca cggtcgtgcg gcaggccccg tcgggaagga agcgctggtc gatggacgcc
     5461 cccgcgagat cgtccgcgaa cccggccccg cgctcgaccg cgccgcggtt gcgcaggatc
     5521 agccaggtga tggcccggta gatcgcgccc gaatcgacat aggaaatccc cagccgcttc
     5581 gcgacctcct tcgacacgct gctcttgccc gagcccgccg gaccgtcgat ggcgacgatg
     5641 atgtgatcac tgcccatgaa gaatgctctc ctgcggttca atgccgcgga cgaaccgctc
     5701 atccgctgcg ctatgctgcg cgcagcacca aaccccggcc atgaagccgg ggccgaacat
     5761 cgcataccaa ttgaatatca ataacagtct ctgccgattg ccttcgcgac cttgttcacg
     5821 cgcgcgatga ggtccgtgag ctgttcgtag ttgacggtct gctggccgtc ggaggtggcg
     5881 tcttcggggt tcgggtgcgc ctcgacgatg agcccgtccg cgcctgcggc gatataggcg
     5941 agtccggcgg ggataacgta atccctgatt cccatggcat ggctcggatc gcagatgacc
     6001 ggaagatggc tcagctcctt gaccacgggc acggcgccgg cgtcgagggt gttgcgcgtg
     6061 atgtcctcga aggtgcggat accgcgcggg cacagaatga cattgcggtt tccctgcgcg
     6121 agaatgtatt cggccgacat gaggaactcg ttgatggtcg cgttcatccc gcgcttgagg
     6181 agcaccggcg ttcgcatccg gcccacctcc ttgaggagat tgtagttctg catgttgcgg
     6241 gtgccgattt gcatcatgtc cgcgtgctcg gcgaggaact cgacgtcgcg cggatccatc
     6301 acctcggtca cgaacggcag tcccgtctct tggcgcgcgg cgaccatgaa catcaccccc
     6361 tcgcgcccgt aaccctggaa cgaatacggc gaggtgcggg gcttgaaggc gccggcgcga
     6421 aacatcttcg ccccggcctt cttgatcatg cgcgcgtgct ccacgaccgc cgcttcgccc
     6481 tcgacggcgc agggaccggc catcacgacc accctggggc cgccgatctc cacatcgccg
     6541 accttgatga cggtgcggtc gggatgcgtc tccttgctca cgagcttgaa gggccgcagg
     6601 atggacttga ccgattcgac gcccgccagc gcctccagcg gctgctcgcg gatgatgtcc
     6661 tcgtcgccga tgacggtgat gatggtccgc tggacgccgg tggatacgtg ggggttcagc
     6721 ttgagcgact tgatcttctc gacgatgtgg tcgatctcgt cctgggtaac cccgggtttc
     6781 aatacgatga gcacggcatt tcactccaga tgacgatcat ccccacaccc gcgacgggcc
     6841 tcggagggac aatgcagtat gttcgccagt cagttcgcgg cgggatacga accgaggatt
     6901 ttgaaaaaca gggtttcctg cttcataata tcgaccgcag cgcgcacaac cgtatcattg
     6961 gtatgaccgt tgaagtcaat gaaaaaattg tactcccaca tgcttttctt gtccggccgc
     7021 gactcgatct tggtcatgtt gatccccgcc tcgtggaagg cgcgcagaag atcgtagagc
     7081 gccccgggct tgtccttcac cgcacacacg atggaggtct tgtcgttgcc ggtgggcgga
     7141 ttgtcctttt tccccagcac caggaagcgc gtgtagttgt tcttggaatc ctcgatgttc
     7201 cggtcgagca cgttgagcga atagatgcgc gcggagatct ccgatgagat ggccgcggag
     7261 ttcttgtccc acgacgccgt ctccgccgcg agcgagttcg aggcgacgtt gtgcatctcg
     7321 gcgcccggca ggttcttccg gatccacgac ttgcactgcg cggccgcctg gggatgcacg
     7381 tagagcttct tcacgccgcg gatatcgggg cacagcgaca ccaggcaata ggagatgcgc
     7441 aggaattttt cgcacacgat cttcaggtcg gtgtcgagga gctcgtcgag ggtgtaggtg
     7501 accgcaccct ccgtgctgtt ctccacgggg acgatcccga agggcgcctt gtccatctcc
     7561 acctcgagga atacgtcggg gatggtcttc tgggggacgg cctcgaccga ttcgccgaac
     7621 acctcgagga gggcgatctg ggtaaacgtt ccctccggcc c
//
LOCUS       DAORHF010000179         7657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9195_length_7657_cov_5.810182, whole genome shotgun sequence.
ACCESSION   DAORHF010000179 DAORHF010000000
VERSION     DAORHF010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7657
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9195_length_7657_cov_5.810182"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="PKJ16_13475"
     CDS             complement(<1..724)
                     /locus_tag="PKJ16_13475"
                     /inference="COORDINATES: protein motif:HMM:NF039484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48048.1"
                     /translation="MKSKLKDFSQKHYYLLTGILWFLSVPSFDAWFLKGFPFFAWIAF
                     VPLFVYVRDKGYREVFRASFMAGLLGNLLAYQWIGAFGAKVPGGYFVVLLFLIPSLTV
                     FFVSGMMMAEFLSRRVERLRAPIYAGSFVVVYWVQSMGYLAYPMNYWGYSQFPFTPFI
                     QVSSIVGIMGVCFILIFFNESMAGLIARIASGARSFREAIASFEGKAFVGALALVAGI
                     TAWGAVTLAANPATGKRDLRALM"
     gene            complement(779..2215)
                     /locus_tag="PKJ16_13480"
     CDS             complement(779..2215)
                     /locus_tag="PKJ16_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48049.1"
                     /translation="MPQTVSPDARRKDIKRLVILPFENMGAHSKEFDSIGEEMSISLI
                     QSLSQVKELSVMDPTSVRKLLVEKLEFIQGSGIGDDKKLKREIEKIDEENFDLILLGN
                     YRIIGDKIKVTLKLLERENNRVAITPISVMGSYPGQLFSVEEDIAEKAAVAIDIYNKA
                     YVVDFKNFYAFTTNRESYRLYLMGINAKSDLTIQGYMKAVMLFYRSYMYDASFFLPLY
                     ELENLKNDIAVTISKLGLMRNMFNSFGNYDAHKVLIELLSENKITGIINACYKARAEY
                     REKNSANFTRKEQIFSLSQHFNKYRDPRKDAINHPNDIEILFRAWEKVDKFDVNSDYK
                     IKIKSIEKNYMPLKIIELFNEINGKFEQNKKVILGLQKEVGSIQKSNEYSNKQNLLSS
                     RIKNVIDAEVEYFLSKTDGFEKMYPKSLYNLTYYEYFLRYSFKILEAKMYFVENVDYE
                     NSKEGVRFKTNLRHFYNAISEEAPLVRP"
     gene            complement(2662..4665)
                     /gene="ligA"
                     /locus_tag="PKJ16_13485"
     CDS             complement(2662..4665)
                     /gene="ligA"
                     /locus_tag="PKJ16_13485"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HNV48050.1"
                     /translation="MAPERETKRYEELVALIERYNHHYYELDSPLVDDAEYDAAMREL
                     AALEAAHPELAREDSPTRRVGGARSSTFGEVRHDPPMLSLGNVFDAAELADFDERCRK
                     LLKADGSLVYTAELKFDGLAVEIVYRDGRYAQGSTRGDGATGEDVTANLATIRALPAR
                     LAGARVPAYLSVRGEVFMRHDEFERLNAERLAAEEPPFANPRNAAAGSLRQLDTGVTA
                     SRALDIALYGIGRIDPPDSAHGQGALFDLLSELGLPVAEHRFRGELDGVVAFYERWRD
                     GRHRLNFDIDGIVIKLDDFAARERVGATSKAPRWAVAWKFPAKEAVTTIVSVDLQVGR
                     TGVITPVANLAPINIGGVIVKRATLHNFDEVRRLDARVNDTVTVKRAGDVIPKVVAVL
                     VDSRPNDARAIEPPERCPGCGKELAREEIYIRCVNPACPAKALETLKFFVSKDGMDIE
                     FFGPELMQRLYAAGIVKTFADVFRLTRDDLAGVERMGEKSAERIIESVRARRRIGLSH
                     FLRSLGIRNVGDHVAKVIARAAGSLDALRAMGADELMAVHEVGPGVAESVSQFLADPA
                     LRAIVDDLVAAGVEVADEEAPAPPASAVAGKTFVVTGTLASLSRQEAEALIEKRGGRA
                     AGSVSKKTDYVVAGESAGSKLARAKELGVTVLDEDAFLKMMRD"
     gene            complement(4673..5995)
                     /locus_tag="PKJ16_13490"
     CDS             complement(4673..5995)
                     /locus_tag="PKJ16_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48051.1"
                     /translation="MLAYVARNAVLIARTVLRRVPGALVSSFGIVFFIAFLIVFIALR
                     TGVRDYLENRIFSRLNINEIRVTPKSKTTLINLNIFSAEKTRIPEGAARRIQRLEGVA
                     KVHRVVRLDVPVTMTFGMFGALIKSDMLISGIDRDFFRGSKIPWRVPKDDEMVPVVVP
                     YFALELYNNFANAAGTPEMGEKFILGKTMELTFGKSFMFGSGGLLFGRSGPERRTVIA
                     QGQIVGFTSQLNTAGLVLPAEYIRALCRKNPDLARRPGECTSNMMLIVRVSDPRHLPA
                     VSQRIRDLGLAVESKEDIAQRTQKAVGFIDAVFFMLLMIILALTVIAIFNSYLTIVYN
                     RSYEISLERMLGATKVRIIAIFIIEAAIVGLVYGLAGFLLGSLAVTKLAHYAGNWVPL
                     LGGLTISFNASAYAAMALGASVAVSALSALVPAVFASNLSLFDNVKRF"
     gene            complement(6004..7092)
                     /gene="prfB"
                     /locus_tag="PKJ16_13495"
     CDS             complement(6004..7092)
                     /gene="prfB"
                     /locus_tag="PKJ16_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156801994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HNV48052.1"
                     /translation="MREIEALQAKVNDLYASINVDALRSKLAALEAKASADDAWQDRE
                     RLATLNREISALKKRIEPWDRLMGTIGENRELMEIALAESDEGVLAETAASLGALAAE
                     FDRIETIELLSGEDDLRDCFVTVRPGAGGTESQDWASMLFRMYLRWAEDNGYRASVID
                     YTPGDEAGVKSATVNVQGDYAYGFLKGERGVHRLVRISPFDANKRRHTSFASVEVVPE
                     VADDIAIDINESDLRIDTYRSSGAGGQHVNTTDSAVRITHLPTGIVTQCQNERSQHKN
                     KAFAMKVLRAKIYELKREEMDRKREEKAGEKKDISWGNQIRSYVFQPYMMVKDHRTGE
                     ETSSVDSVMDGAIDRFIYAYLKNRRAAG"
     gene            7370..>7657
                     /locus_tag="PKJ16_13500"
     CDS             7370..>7657
                     /locus_tag="PKJ16_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48053.1"
                     /translation="MRIRYASAIARTVLVIVIGAAACCATRPGSLPTAVPTGEWRYAL
                     TINGTPAGSSRYSNAIVGPHYRSIVEITMTLGDAVNTSRSEITETLDHVPVR"
BASE COUNT         1574 a         2373 c         2125 g         1585 t
ORIGIN      
        1 ccatcagcgc ccggaggtcg cgcttccccg tcgcggggtt cgcggcgagc gtcacggcgc
       61 cccatgcggt gattcccgcg accagcgcga gcgccccaac gaacgccttc ccctcgaacg
      121 atgcgatcgc ttctcgaaac gagcgcgcgc ccgacgcgat gcgcgcaatc aggcccgcca
      181 tgctttcgtt aaaaaatata agaatgaagc acacgcccat gataccgacg attgaagaaa
      241 cctgaatgaa gggtgtgaag ggaaactggg aatagcccca gtagttcatg ggataggcga
      301 gatatcccat cgactggacc cagtagacga ccacgaacga cccggcgtag atcggcgcgc
      361 gcaggcgctc gacgcgacgc gagaggaact ccgccatcat catgcccgac acgaaaaaga
      421 ccgtgaggct gggaatgagg aagagcaata cgacgaaata cccgccgggg accttggcgc
      481 cgaaggcgcc gatccactga taggcgagca gattgcccag cagcccggcc atgaacgatg
      541 cgcgaaacac ctcgcgatat cccttgtcgc gaacatagac aaagagggga acgaaggcga
      601 tccacgcgaa aaagggaaat cccttcagga accacgcatc gaagctgggc accgaaagaa
      661 accataggat gccggtgagg aggtagtagt gtttttggga gaagtctttg agttttgatt
      721 tcatatgatt gcatccattt taatatttaa aaattagcgg actattatag cgattttttt
      781 acggccggac aagaggcgct tcttcactaa tcgcattata aaaatggcgc aaattcgttt
      841 taaacctaac tccctctttt gagttctcat aatctacatt ttcaacgaaa tacattttag
      901 cttcgagtat tttaaaagaa tatctcaaga aatattcata ataagtcaga ttgtataatg
      961 acttaggata catcttttca aaaccatcgg ttttactaag aaaatattca acctcggcat
     1021 ctataacatt ttttattctc gatgataata gattttgttt attggaatat tcgttacttt
     1081 tctgaatact cccgacttcc ttttgaagcc caaggatcac ttttttgttt tgttcaaatt
     1141 tcccattaat ttcgttaaat aactcaatga ttttgagagg catataattc ttttcaatcg
     1201 acttgatttt aattttataa tcactattca catcaaactt gtccactttt tcccatgcac
     1261 gaaaaagaat ttcaatgtca ttaggatgat ttattgcatc cttcctcgga tcacgatatt
     1321 tattgaaatg ctgcgacaag gaaaatattt gctctttcct cgtgaagttc gcactattct
     1381 tttctcgata ctccgctctt gctttataac acgcattaat aatgccagta attttgtttt
     1441 ccgacaacaa ctcaattaac actttatgcg cgtcataatt accaaacgag ttaaacatgt
     1501 ttctcatcaa tccaagctta gagattgtca ctgctatgtc gtttttcaga ttttccagct
     1561 catataaggg gagaaaaaat gatgcatcgt acatatacga tctgtaaaac aacatgacag
     1621 ctttcatgta gccttgaata gttaaatcgc tttttgcatt gatccccatg agatacaatc
     1681 gatatgattc gcgatttgtt gtgaacgcgt aaaagttttt aaaatcaacg acataagcct
     1741 tattgtatat atctattgcc accgctgcct tttctgctat atcctcctca acactaaaca
     1801 actggcccgg gtaacttccc atcacgctaa tcggcgtgat ggccactctg ttgttctcgc
     1861 gttcaagcaa ttttagcgtt actttaattt tgtctccaat aatcctataa ttgccaagga
     1921 gaataagatc aaaattctct tcatcaattt tttctatctc tctttttaat ttcttatcat
     1981 ccccaatacc agacccttga ataaattcca acttttctac aagcagcttt cggactgacg
     2041 ttggatccat gacactaagt tccttaactt gtgaaagcga ctgaatcaag ctaattgaca
     2101 tttcctcgcc gatggagtca aactcctttg aatgcgcacc catattctca aatggaagta
     2161 tcacaagccg cttgatgtct tttcttcttg catcaggaga aacagtctgc ggcataaata
     2221 taattatcga taaagcaact attaaacatt tgatgatata tcggataaca ttcaatgtgc
     2281 tatttgctgt aattcgtacc atgtcttcga cctttactgc ctagttcttg ttctgtttat
     2341 tgattcgtat atacaggcgt gtggccccga aaaattttaa cacttcctat tacatcctcg
     2401 atcatatatt cctggtgcat cgtgcgcgac aagtaccacg ggtcaatccg atactatctt
     2461 ttaagatgac aatagaaaac aaacaaagaa aagccactcg ctcaacctca ccccgcctcc
     2521 cctcgacgaa gctcggggaa caccggcgcg ccctctcccg gaggagaggg ctccttggaa
     2581 aagaattctt gccttttctc cccttctcct ttgggagaag gggccggggg atgaggtagc
     2641 cgtccctatc agaataaaat ttcaatcccg catcatcttc aaaaacgcgt cctcgtccag
     2701 caccgtcacg ccgagctcct tggccctggc gagcttcgat ccggccgatt ccccggccac
     2761 cacgtaatcg gtcttcttgc tcaccgatcc cgcggcgcgc cctccccgct tctcgatgag
     2821 cgcctcggcc tcctgccgcg acaggctcgc gagggtcccg gtcaccacga aggtcttccc
     2881 ggccacggcc gatgccggcg gggcgggcgc ctcttcgtcg gcgacctcga cgccggcggc
     2941 gacgagatcg tccacgatgg cgcgcaacgc cggatcggcg agaaactgcg agaccgattc
     3001 cgcgacgccc gggcccacct cgtggaccgc catgagctcg tcggcgccca tcgcccggag
     3061 cgcatcgagg ctccccgcgg cccgcgcgat caccttcgcg acgtggtcgc ccacgttccg
     3121 gatcccgagg ctccgaagaa aatgcgacag gccgatccgc cgccgcgcgc gcaccgattc
     3181 gatgatccgc tctgcgctct tctcgcccat ccgctccacg cccgcaagat cgtcgcgcgt
     3241 gaggcgaaag acgtccgcaa acgtcttcac gatgccggcg gcgtagagcc gctgcatgag
     3301 ctccggcccg aaaaactcta tatccatccc gtctttggac acgaaaaact tgagcgtctc
     3361 cagcgccttc gcgggacagg ccggattaac gcagcgtatg tatatctcct cgcgcgcgag
     3421 ctccttcccg cagccgggac agcgttcggg cggctcgatt gcgcgcgcgt cgttcggacg
     3481 ggaatcgacg agcaccgcca ccaccttggg gatcacgtcg cccgcgcgct tcaccgtgac
     3541 ggtgtcgttc acgcgcgcgt ccaggcggcg cacctcgtcg aagttgtgga gcgtcgcgcg
     3601 cttcacgatc accccgccga tgttgatggg cgcgaggttc gcgaccgggg tgatcacgcc
     3661 cgtgcgtccc acctggagat cgacggacac gatggtggtg accgcctcct tcgcggggaa
     3721 cttccacgcg accgcccagc gcggcgcctt gctggtggcc ccgacgcgct cgcgggccgc
     3781 gaagtcgtcg agcttgatca cgatcccgtc gatgtcgaaa ttgagccggt gccgcccgtc
     3841 gcgccagcgc tcgtaaaaag ccacgacgcc gtcgagctcg ccgcggaagc ggtgctccgc
     3901 gaccggaagc cccagctcgc ttaaaagatc aaagagcgcg ccctggccat gagcggagtc
     3961 cggcggatcg atcctcccga tgccgtagag cgcaatgtcg agtgcgcgcg aggcggtgac
     4021 gcccgtatcg agctggcgca gcgacccggc ggccgcgtta cgcggattcg cgaacggcgg
     4081 ctcctccgcc gccagccgct ctgcattgag ccgctcgaac tcgtcgtggc gcatgaacac
     4141 ctcgccgcgg acggagagat aggccggaac gcgcgccccg gcaagccgcg cgggcaatgc
     4201 gcggatcgtc gcgaggttcg ccgtcacgtc ctcgcccgtc gcgccgtcgc cgcgcgtgga
     4261 gccctgcgcg tagcgaccgt cgcgatagac gatctcgacc gcgaggccgt cgaacttgag
     4321 ctctgcggtg tagacgagcg agccgtccgc cttcaggagc ttccggcagc gctcgtcgaa
     4381 atccgcaagc tccgccgcgt cgaatacgtt ccccagcgag agcatgggcg ggtcgtggcg
     4441 cacctcgccg aaggtggacg atcgcgcgcc gcccacgcgg cgcgtcgggg agtcctcccg
     4501 cgcgagctcc gggtgcgccg cctcgagcgc ggcgagctcg cgcatcgcgg cgtcgtactc
     4561 ggcgtcgtcc accaggggcg aatcgagctc gtaataatga tgattgtacc gctctatgag
     4621 cgcgacgagc tcctcatacc gctttgtttc gcgttccggc gccatggaca actcaaaagc
     4681 gcttcacgtt gtcgaagagg ctcaggttcg acgcaaagac cgcgggcacg agcgccgaga
     4741 gcgcggacac cgccaccgag gcgccgagcg ccatcgccgc gtacgcgctc gcgttaaacg
     4801 aaatcgtaag cccgcccaac agcgggaccc agttcccggc gtagtgcgcg agcttcgtga
     4861 ccgcgagcga ccccagcagg aagcccgcca gcccgtagac gagccccacg atggccgcct
     4921 cgatgatgaa gatggcgatg atccgcacct tggtggcccc cagcatccgc tccagcgaga
     4981 tctcgtagct gcggttgtac acgatcgtga gataggaatt gaagatcgcg atcacggtga
     5041 gcgcgaggat gatcatgagc agcatgaaga agacggcgtc gatgaagccc acggccttct
     5101 gggtgcgctg cgcgatgtcc tccttcgatt ccacggcaag ccccaggtcg cggatccgct
     5161 gcgagaccgc cggcaggtgg cgcggatcgg acacccgcac gatgagcatc atgttcgagg
     5221 tgcattcccc ggggcgccgc gcgaggtccg ggttctttct gcacagcgcg cggatatact
     5281 cggcggggag cacgagcccc gcggtgttga gctgcgaggt gaacccgacg atctgcccct
     5341 gcgcgatcac ggtgcggcgc tcggggccgg agcgcccgaa gagcagcccg cccgagccga
     5401 acatgaagga cttgccgaag gtgagctcca tggtcttgcc caggatgaac ttctcgccca
     5461 tctccggagt acccgcggcg ttcgcgaaat tattgtacag ctccagcgcg aagtacggca
     5521 ccaccacggg gaccatctcg tcgtccttcg ggacgcgcca ggggattttt gatccgcgaa
     5581 agaagtcgcg gtcgatgccg ctgatgagca tgtcggactt gatgagcgcg ccgaacatgc
     5641 cgaaggtcat ggtcacgggc acgtcgagcc ggacgacgcg gtgcaccttc gccacgccct
     5701 ccaggcgctg gatgcgccgc gcggcgccct cggggatccg cgtcttctcg gcggagaaga
     5761 tgttcaggtt gatgagcgtg gtcttgcttt tcggcgtgac gcggatctcg ttaatgttga
     5821 gccggctgaa gatgcggttc tccaggtagt cccgcacccc ggtgcgcagg gcgatgaaca
     5881 cgatgaggaa cgcgatgaag aacacgatcc cgaagctcga cacaagcgcc ccggggaccc
     5941 gccgcaggac ggtgcgggcg atgagcacgg cgttgcgcgc aacatatgcc agcatggccg
     6001 cccctatccg gccgcgcgcc ggttcttgag ataggcgtag atgaaccggt cgatcgcgcc
     6061 gtccatcacg ctgtccaccg agctcgtctc ctcgccggtg cggtggtcct tgaccatcat
     6121 gtacggctga aacacgtacg agcggatctg gttgccccag gagatgtcct tcttctcgcc
     6181 cgccttctcc tcgcgcttgc ggtccatctc ctcgcgcttg agctcgtaga tcttggcgcg
     6241 caggaccttc atcgcgaagg ccttgttctt gtgctgcgaa cgctcgttct ggcactgcgt
     6301 cacgatgccg gtggggaggt gggtgatccg caccgcggag tcggtcgtgt tcacgtgctg
     6361 cccgccggcg ccggaggagc ggtaggtgtc gatgcgcagg tcggattcgt tgatgtcgat
     6421 ggcgatgtcg tcggcgacct cgggtaccac ctccaccgac gcgaaggagg tgtgccggcg
     6481 cttgttcgcg tcgaagggcg aaatccgcac caggcggtgg acgccccgct cgcccttgag
     6541 gaagccgtag gcgtaatcgc cctgcacgtt cacggtggcg ctcttcaccc cggcctcgtc
     6601 gccgggggtg tagtcgatca cgctcgcgcg gtagccgttg tcctccgccc aacgcagata
     6661 catgcgaaag agcatgctcg cccagtcctg cgattcggtg ccgcccgcgc cgggtcggac
     6721 cgtcacgaag caatcgcgca ggtcgtcctc gcccgacaac agctcgatcg tctcgatgcg
     6781 gtcgaactcc gcggcgaggg cccccaggga tgcggcggtc tcggccagca cgccctcgtc
     6841 ggattcggcg agcgcgatct ccatgagctc gcggttctcc ccgatcgtgc ccatcaggcg
     6901 gtcccagggt tcgatgcgtt ttttgagcgc gctgatctcc cggttgaggg tcgccagccg
     6961 ctcccggtcc tgccaggcgt cgtccgcgct cgccttcgcc tccagcgccg caagcttcga
     7021 ccgcagcgcg tccacgttga tcgacgcgta gaggtcgttc accttcgcct ggagcgcctc
     7081 gatctcgcgc agtgattcct gataatcctt ctcggccacc ggttgcatcc ctagtccctg
     7141 caatgagcgc agataatgga acaggcggga aatacggtca agacatttta ggggccgatc
     7201 ccctgcgggc gctgcgccac cacacgcacg aaccgaccgc gcaacgggcc gcagggatct
     7261 tcccccggcg cgtgccgacg cggcatgccc gcgagcggcg gattgcgctt gacatcacgc
     7321 cgtcgcgcaa tcaatgatcc gacaatcccc cgcaaaggac aacacgccca tgcgaatacg
     7381 atatgccagc gcgattgcgc gcaccgtcct cgtgatcgtc attggggcgg ccgcctgctg
     7441 cgccacgcgc cccgggtcgc tcccgaccgc cgtgccaacc ggcgagtggc gctacgcgct
     7501 caccatcaac gggacccccg cgggctcgtc ccgctattcg aacgccatcg tgggaccgca
     7561 ctaccggtcg atcgtcgaga tcaccatgac gctcggcgat gcggtgaaca cctcgcgcag
     7621 cgagatcacc gagacgctgg accatgtccc ggtgcgg
//
LOCUS       DAORHF010000180         7568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9362_length_7568_cov_5.031812, whole genome shotgun sequence.
ACCESSION   DAORHF010000180 DAORHF010000000
VERSION     DAORHF010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7568
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9362_length_7568_cov_5.031812"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="PKJ16_13505"
     CDS             <1..737
                     /locus_tag="PKJ16_13505"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV48054.1"
                     /translation="HSGILTAGYSTFELFKEIKLMAKSSVSALYSREKFYVMGKDETV
                     YYIEYFNAGESLMNKPMLSRFDDAGVLSFQLAAEEGRYNPSTKLWDFKKINLLTFDAE
                     KNLIEKKLESMSMSLSEDPKAFMQTPENMSQMRFGDALNFIEEKKKIGVNHRQYLVEF
                     QRRFTFPFSIIIVILIGGVAGIYFKKAVLVMALLLSIAISFAYYGMMAVGMAFGNSGK
                     VHPVVAAWFANAVFLLAGIVIVRLKR"
     gene            745..1557
                     /locus_tag="PKJ16_13510"
     CDS             745..1557
                     /locus_tag="PKJ16_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015902858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HNV48055.1"
                     /translation="MPDDSTYTKAFLIDMDGVINKGATLIPGAREFLQRLRDGGHPYL
                     FITNNSYYTPAELRDRLGRIGIDEDEKRFYTSAMATATFLEYQMPRCTAYVIGGNGLI
                     HELEKRGIAITDKNPDYVIVGETDKYDYRRIVEATHLIQEGARFIATNQDITGPTPRG
                     PVPACGALVAPLERVTGVKAYFLGKPNPAMMFWARKKLRVHSANSFMVGDRMETDILG
                     GLEAGMTCCLVLSGVTSREMLARYPYLPDYVFNHVGEIDPDIILSRRERVQE"
     gene            1563..2852
                     /locus_tag="PKJ16_13515"
     CDS             1563..2852
                     /locus_tag="PKJ16_13515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV48056.1"
                     /translation="MNTHARGISSRWLFPGILAPVVLAVAAGVFLRATANSGAVRNLH
                     LVFHDPRARAVTVVWNTPHRLERPVVRYHQVSGDDSAPCAHAAVAPEIEQPAVALRDT
                     GSRDWRYRATLAGLEPGARYCYRIADSGGAIVDYRAFRSAVRPGEPFTVLAFGDSRQS
                     KRHRNSPIRRSLADAIGREDLPHLTIFTGDMVDDGSSYRQWVQWFADFSPVFERSPLV
                     PVYGNHEAKENKHEVVYYRFFPSPGGGRWFSLDYGNLHLVVLNTEVPYSASPWSMWQE
                     QARWFAQDMERAAARPDILWRVVACHKPPYSTGHHAVEKDSRDVQRHLPPLFDAHGVD
                     LVLSAHDHYYQRSYPLRNGRVTDRSASVYRNPRGVMYLVTAGAGAPLTHGAPAEWVAK
                     YREAYHYCLIAFGPGRELVITATDINGDLIERVRIVK"
     gene            complement(2863..3744)
                     /gene="tatC"
                     /locus_tag="PKJ16_13520"
     CDS             complement(2863..3744)
                     /gene="tatC"
                     /locus_tag="PKJ16_13520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HNV48057.1"
                     /translation="MTEPMHKEHTRSQTPAKRTSLLRSKRSRTPAPPPETPEETENID
                     PDALRRGDVPMDMVDHLGELRSRLITVIVALALSVGGCFMLSDYILAYISKPFTATGL
                     KLHVFAVTEGFLIRVKTALITGIIAALPIIVHQLWRYIMPAVDVRDRRFARFSIAAAL
                     ALFFAGTAFTLVAVLPASIRFLVSLTPTGMQNLQNASDYISFVLMFCAGIGVLFEFPL
                     VIMILARIGIITPQFLASRRRAAIVLIFVGAAIFTPPDILSQVIIALPLVLLYELAII
                     IARFVIMRKKKRELQRA"
     gene            3934..4206
                     /locus_tag="PKJ16_13525"
     CDS             3934..4206
                     /locus_tag="PKJ16_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HNV48058.1"
                     /translation="MPTYEYECTECKHRFDVFQKMSDEPVKECVKCTGSVRRVINGGS
                     GIIFKGSGFYVNDYKKDTKCGSSSSADAPKQSESCAACSAADSCGA"
     gene            complement(4208..6061)
                     /locus_tag="PKJ16_13530"
     CDS             complement(4208..6061)
                     /locus_tag="PKJ16_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48059.1"
                     /translation="MAKLNLDQIVQKYPFLERHVAKEPSIGEVLDVILEHQDRSRPEF
                     SLPLATMDVLLTTRAINIPYTTARDRNIGYLVDLLTKTYPEQVRPVATFAFSVDEATK
                     KLSIQETVEYTVMLDREFMDRLVAQRDAASLENLLGLLARFRFFNLTKLIKKAKRKDD
                     LSRIIKMELGKQIFTFKHYDLDKDLLEWAEDHCFHAKNFKAVQESFTLKALREIIAKY
                     SDFVNRRLKKYGILNADFSDYRDSKLDYLFNILIDDIATTMADRDLVEVKNMQSLRKC
                     LQKVETLLDPTQRLGTDIAAHVRDAGLCTEQEIMHAVPEVTHEIVEKWATDETLAAAR
                     IVRTAGDDGSVAFINAPKLVSLLVESAGLYARQPENLAQLSHLEKQQVEARARILVSA
                     ARAILNSGKNIRELLGADDEEIAQVRSLIADYEDAIKRQVLKKELERKDRRPTRKRSF
                     FQVIIDFFKSLFGARQSGGAPSESDGAPARKKLTRQAVLVYQKTASKNAPILPLSEFI
                     ELTPANSDIIDSVIDELRESNTRVVIPIYNARKSLYPKRSQKLLIADVEYLLIPPKTM
                     KSPDSIREYIDSLVGYNLKDEPIPGNTLLTIEKQLLGMYHQKRQMALKKEL"
     gene            complement(6130..7398)
                     /gene="hisS"
                     /locus_tag="PKJ16_13535"
     CDS             complement(6130..7398)
                     /gene="hisS"
                     /locus_tag="PKJ16_13535"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HNV48060.1"
                     /translation="MLKKPPGIEDIFPDAMEKWDHVTGTARDVFRTYNYKELIIPVME
                     YTEVFARGLGDETDIVSKEMFTFEDRGGRSLTLRPEGTASVVRAYVENGEYNRLASCK
                     LFYCGPMFRAERPQKGRLRQFNQFGAEFFGSENPYHDAEVIGMMDAISRRLALAPYAV
                     LVNSIGCPDCRVPYTEALKSFYTSHSARLCEDCARRLEKNPLRLLDCKNESCRALRAE
                     APVITAFICGACRDHHDRVKGHLSQAGVSFTEDPFLVRGLDYYTRTTFEFVTERLGGQ
                     NAFAAGGRYDGLVEQFGGKKTPAVGFAAGIERILLMLGDQRAPERRLDVYIVHSGGDA
                     LALAARTAAALRERGIAADLDPDAGGFKSQFKKADRERARLCVIVGEDELREGTRSIK
                     DMGSGEQASVPADAAADRIGAMLKGSASLP"
BASE COUNT         1350 a         2335 c         2411 g         1472 t
ORIGIN      
        1 tgcacagcgg gatcttgact gcgggctatt caaccttcga gttgtttaaa gagatcaagc
       61 tcatggccaa aagcagcgtg agcgcgctgt attcccgcga gaagttttac gtcatgggga
      121 aggacgagac ggtgtattat atcgaatatt tcaacgcggg cgaaagcctc atgaacaagc
      181 ccatgctcag ccggttcgac gatgccggcg tcctctcgtt ccagctcgcc gccgaggagg
      241 gccgctacaa cccctcgacg aaactctggg acttcaagaa gatcaatctg ctcaccttcg
      301 acgccgagaa aaatctcatc gagaaaaagc tcgagtcgat gtcgatgtcg ctgtccgagg
      361 atcccaaggc ctttatgcaa acccccgaaa acatgagcca gatgcgcttc ggcgacgcgc
      421 tcaatttcat cgaggagaag aagaagatcg gggtcaacca ccgccagtac ctggtcgagt
      481 tccagcgacg cttcaccttt cccttctcca tcatcatcgt catccttatc ggcggcgtgg
      541 ccgggatcta cttcaagaag gcggtcctgg tgatggcgct gctgttgtcc atcgcgatct
      601 ccttcgctta ttacgggatg atggccgtgg gcatggcctt cgggaattcc ggcaaggtcc
      661 atccggtcgt cgcggcgtgg tttgcgaacg ccgtcttcct cctggccgga atcgtcatcg
      721 ttcggctcaa gcggtagcgg cgccatgccc gacgacagca cctacaccaa ggcctttctc
      781 atcgacatgg acggcgtgat caacaagggg gccaccctca tcccgggcgc gcgcgagttc
      841 ctgcaacgcc tgcgcgacgg cggccacccg tatctgttca tcaccaacaa ttcctattat
      901 accccggccg agctccggga ccggctcggg agaatcggca tcgacgagga cgagaagcgc
      961 ttctatacgt cggccatggc gacggcgacc tttctggagt atcagatgcc gcgatgcacg
     1021 gcatatgtga tcggcgggaa cggcctcatc cacgagctcg agaagcgggg catcgccatc
     1081 accgataaaa accccgatta cgtcatcgta ggcgaaaccg acaagtacga ctatcgcagg
     1141 atcgtcgagg ccacccacct catccaggag ggggcgcgct tcatcgccac caaccaggac
     1201 atcacgggac ccacgccgcg cggtccggtc cccgcctgcg gggcgctggt cgcgccgctc
     1261 gagcgggtga cgggcgtgaa ggcgtatttt ctgggcaaac ccaacccggc gatgatgttc
     1321 tgggcgcgaa agaagctgcg cgtccactcg gcgaacagct tcatggtggg cgatcgcatg
     1381 gagaccgaca ttctgggcgg cctggaggcg ggcatgacct gctgcctggt cctctccggg
     1441 gtaaccagcc gagagatgct cgcgcgctat ccgtatcttc ccgactatgt attcaatcat
     1501 gtcggcgaga tcgatcctga cataattctc tcccgccgcg agcgggtcca ggagtagtcc
     1561 gcgtgaatac acacgcacgg ggcatatcgt cgcgatggct gtttccgggc attctcgccc
     1621 cggtcgtact cgccgtcgct gcgggcgttt tcctgcgcgc cactgcgaac agcggcgcgg
     1681 tccgcaacct ccatctggtg tttcacgatc cacgcgcacg tgccgtcacg gtcgtatgga
     1741 atacgccgca tcgtctcgaa cgaccggtgg ttcggtacca tcaagtgtcg ggggatgatt
     1801 cggcgccgtg cgcgcacgcc gccgtggccc cggagatcga acagccggcc gtagcgctgc
     1861 gcgacaccgg ctcgcgcgac tggcgctacc gggcgacgct tgcgggcctc gaaccggggg
     1921 cgcggtactg ctatcgaatc gcggactccg gcggcgccat cgtcgattat cgcgcatttc
     1981 gaagcgccgt tcgtccgggg gagcccttta cggttctcgc gttcggcgat tctcgtcagt
     2041 ccaagcggca ccggaactcg cccattcgcc gctccctcgc cgacgccatc gggcgcgagg
     2101 acctcccgca tctcacaatt ttcaccggcg acatggtgga cgacggatcg tcgtatcggc
     2161 agtgggtcca gtggttcgcg gacttctcgc ccgtcttcga acgcagcccg ctcgtcccgg
     2221 tctacggcaa tcacgaggcg aaagagaaca agcacgaggt cgtgtactat cgtttttttc
     2281 cgtcgcccgg cggcgggcgc tggttttcgc tggactacgg gaacctgcat ctcgtcgtgc
     2341 tgaacaccga ggtgccctac agcgcgtcgc cgtggagcat gtggcaggag caggcccgct
     2401 ggttcgcgca agacatggaa cgcgccgccg cgcggcccga catcctctgg cgggtggtcg
     2461 cctgccacaa accgccgtac agcacggggc accacgcggt cgagaaggac tcgcgtgacg
     2521 tgcagcgcca tctgcccccg ctgttcgacg cgcacggcgt cgacctggtg ctgagcgcgc
     2581 acgaccatta ctatcagcgc tcgtatccgc tgcggaacgg ccgcgtcacc gaccgctcgg
     2641 cctccgtcta ccggaacccg cggggcgtca tgtatctggt gaccgccggc gcgggcgcgc
     2701 cgctcaccca tggggcgccg gccgagtggg tggcgaaata ccgcgaggcc taccactatt
     2761 gcctgatcgc cttcggcccc ggccgggagc tcgtcatcac cgcgacggac atcaacggcg
     2821 atctcatcga gcgggtgcgt atcgtcaaat gatacgggcg ccttatgccc gctggagctc
     2881 ccgctttttc tttcgcatga tcacgaaccg cgcgatgatg atggccagct cgtagagcag
     2941 caccagcggc agcgcgatga tcacctggga gaggatgtcg ggcggcgtga atatcgccgc
     3001 gcccacgaag atgagcacga tcgcggcgcg ccgccgggac gcgaggaatt gcggggtgat
     3061 gatgccgatc cgcgcgagga tcatgatcac gagcgggaac tcgaagagca cgccgatccc
     3121 ggcgcagaac atgaggacga agctgatgta gtcgctggcg ttctgaaggt tctgcatgcc
     3181 cgtcggggtg aggctcacca ggaagcggat cgaggccgga agcaccgcca cgagggtgaa
     3241 cgcggtaccg gcgaaaaaca gcgcgagcgc ggccgcgatc gagaagcgcg cgaagcggcg
     3301 gtcgcgaacg tcgacggccg gcatgatgta ccgccagagc tggtgcacga tgatcggcag
     3361 cgccgcgatg atgccggtga tgagcgccgt cttgacgcgg atgaggaacc cctccgtgac
     3421 cgcgaagacg tggagcttaa gccccgtagc cgtaaacggc ttcgatatat aggcaaggat
     3481 ataatcggaa agcataaaac agccgccgac ggatagtgcg agcgcgacga tgacggtgat
     3541 gaggcgcgag cgcagctctc ccaggtggtc caccatgtcc atggggacgt ccccgcgtcg
     3601 cagcgcatcg ggatcgatgt tctcggtctc ctcgggagtc tcgggcggcg gggcgggcgt
     3661 acgcgaacgc ttggagcgca ggagcgacgt gcgcttcgcc ggggtctgtg atcgggtatg
     3721 ttccttgtgc atgggttcgg tcacggcgct gtccttcggg ccatcggcgt ttcatcgccg
     3781 cagcgcggcg gcctgtcaag actatttgcc gcgtccggcc gggggccccg ccgcgcacag
     3841 gaatactgat tgacaataat catcaaaaaa atattttgcc tccgcgtatc cgcgctatgg
     3901 tatgcgcacc agatatcaac cggaggctct cccatgccaa catacgaata tgaatgcacc
     3961 gaatgcaagc atcgattcga tgtctttcag aagatgtccg atgagccggt gaaggaatgc
     4021 gtgaagtgca ccgggtcggt ccggcgggta atcaacggcg ggtccgggat catcttcaag
     4081 gggtccgggt tctacgtaaa cgactacaag aaagacacga aatgcgggtc ctcttcctcg
     4141 gccgatgcgc caaagcagtc ggaatcctgc gccgcctgca gcgccgcgga cagctgcggg
     4201 gcctagccta cagctccttt ttcagcgcca tctggcgctt ctgatggtac atcccgagca
     4261 gctgcttctc gatggtgagc agcgtgttgc ccgggatcgg ctcgtccttg aggttgtatc
     4321 cgaccaacga atcgatgtac tccctgatgc tgtccggcga tttcatggtc ttcggcggga
     4381 tgagcagata ctcgacgtcc gcgatcagga gcttctggct gcgcttggga tacagcgatt
     4441 tccgggcatt gtagatgggg atcaccacgc gggtgttgct ctcgcgcagc tcgtcgatca
     4501 cgctgtcgat gatatcgctg ttggcgggcg tgagctcgat gaactccgat agcggcagga
     4561 tgggcgcgtt cttgctcgcc gtcttctggt acaccagcac cgcttgccgc gtaagctttt
     4621 ttcgcgcggg agcgccgtcg gactcggagg gtgcgccgcc cgattggcgt gccccgaaga
     4681 gcgatttgaa gaaatcgatg atcacctgga agaacgagcg cttccgcgtc gggcgccggt
     4741 ccttgcgctc cagttctttt ttcagcacct gccgcttgat cgcgtcctcg taatcggcga
     4801 tgagcgagcg cacctgggcg atctcctcgt cgtcggcccc gagcagctcc ctgatgtttt
     4861 ttccggaatt gagaatcgcc cgggccgcgg agaccaggat ccgcgcgcgg gcctccacct
     4921 gctgtttctc gaggtgggag agctgggcga ggttttcggg ctggcgcgcg tacaacccgg
     4981 cgctctcgac aagcaggctt acgagcttcg gggcgttgat gaacgcgacg ctcccgtcgt
     5041 cgccggccgt ccggacgatg cgcgcggcgg cgagcgtctc gtcggtggcc catttctcca
     5101 cgatctcgtg cgtcacctcg gggacggcgt gcatgatctc ctgctcggtg cacaatcccg
     5161 cgtcgcggac gtgtgcggcg atgtcggtgc ccagccgctg cgtcgggtcc aggagcgtct
     5221 cgaccttctg caggcacttg cgcagcgact gcatgttctt cacctcgacc aggtcccggt
     5281 cggccatggt ggtggcgatg tcgtcgataa ggatgttgaa caggtagtcg agcttcgagt
     5341 cgcgatagtc gctgaaatcg gcgttcagga tgccgtattt cttgagccgg cggttcacga
     5401 agtcggagta cttcgcgatg atctcccgca gggccttgag cgtgaacgac tcctgcaccg
     5461 ccttgaagtt cttggcgtga aagcaatggt cctccgccca ttccagcagg tccttgtcca
     5521 ggtcgtagtg cttgaaggtg aagatctgct tgcccagctc catcttgatg atgcgcgaga
     5581 ggtcgtcctt tcgcttggcc ttcttgatga gcttggtcag gttgaaaaag cggaaccggg
     5641 cgagaaggcc cagcaggttt tccagcgagg ccgcgtcgcg ctgtgcgacc agccggtcca
     5701 tgaattcgcg gtccagcatc accgtgtact ccacggtctc ctggatggac agcttcttgg
     5761 tcgcctcgtc aaccgagaag gcgaacgtgg ccacggggcg cacctgctcg ggataggtct
     5821 tcgtgagcag gtccaccagg taaccgatgt tccggtcgcg cgcggtcgtg taggggatgt
     5881 tgatggcgcg cgtggtgagc agcacgtcca tggtggccag gggcagcgag aactccgggc
     5941 gcgatcggtc ctggtgctcc aggatcacgt cgagcacctc gccgatagac ggctccttgg
     6001 cgacgtgccg ttcgaggaac gggtatttct ggacgatttg gtcgaggttc agtttggcca
     6061 tgtgcggatc acgtgtttcg cgctctttgc ggtatcgtac cgtccgccaa aaccgctgta
     6121 aacccttttt tacggcagcg aggccgatcc cttcagcatc gccccaatgc gatctgccgc
     6181 tgcgtccgcc gggacgctcg cctgctcgcc cgaacccatg tccttgatgg agcgggtccc
     6241 ctcgcgcagc tcgtcctcgc cgacgatgac gcacagacgc gcgcgctcgc gatcggcctt
     6301 cttgaactgc gatttgaagc cgcccgcgtc cgggtcaagg tcggccgcaa tcccgcgttc
     6361 gcgaagagcg gcggcggtcc gtgcggcgag cgcgagcgcg tctccgcccg aatggacgat
     6421 atagacatcg aggcggcgtt ccggcgcgcg ttggtcgccc agcataagaa ggatccgctc
     6481 aatccctgcg gcaaaaccca cggcaggagt ttttttcccg ccgaactgtt ccaccaggcc
     6541 gtcgtagcgc ccgcccgcgg cgaaggcgtt ctgcccgccg agccgttcgg tcacgaactc
     6601 gaaggtggtc cgggtgtagt aatcgagccc ccgcaccagg aacgggtcct ccgtgaagga
     6661 cacgccggcc tgcgacaggt ggcccttcac gcggtcgtgg tgatcgcgac aggcgccgca
     6721 gatgaacgcg gtgatgacgg gcgcctccgc ccgaagcgcg cggcacgact cgttcttgca
     6781 gtcgagcagg cgcagcgggt ttttctcgag gcgccgcgcg cagtcctcgc agagccgcgc
     6841 ggaatgcgac gtatagaacg acttcagcgc ctcggtgtag gggacacggc aatcggggca
     6901 gccgatcgag ttgacgagca cggcgtaggg tgcgagcgcg aggcggcggg agatcgcgtc
     6961 catcatgccg atcacctcgg cgtcgtgata ggggttctcg ctcccgaaga actccgcgcc
     7021 gaactggttg aactgccgca ggcggccctt ctgcgggcgc tcggcgcgga acatgggccc
     7081 gcagtagaag agcttgcagc tcgcgagccg gttgtactcg ccgttctcga cataggcgcg
     7141 caccaccgac gccgtgccct ccggccgcag cgtgaggctg cggccgccgc ggtcctcgaa
     7201 ggtgaacatc tccttggaga cgatgtcggt ctcatcgccc aggccgcgcg cgaagacctc
     7261 ggtgtattcc atcaccggga tgatgagttc tttataatta tatgtgcgaa acacgtcgcg
     7321 cgcggttccc gtcacatggt cccatttctc catcgcatcg gggaagatgt cttctattcc
     7381 gggtggtttt tttaacattg ggttgtcctg tcgttccgtt tcgcaacggg ttttttaaaa
     7441 ggatgtaaca ccatcaatct tgatgcattg taggtcctgt gcgcttttga ggacgaccac
     7501 ttgaaacttc gatggcagtg agactgcact cacggcttac tccagcatgt cgaggcgatt
     7561 tttgtaga
//
LOCUS       DAORHF010000181         7542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9420_length_7542_cov_6.633231, whole genome shotgun sequence.
ACCESSION   DAORHF010000181 DAORHF010000000
VERSION     DAORHF010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9420_length_7542_cov_6.633231"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKJ16_13540"
     CDS             complement(<1..929)
                     /locus_tag="PKJ16_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV48061.1"
                     /translation="MNIAAFSIKRPIFIMSIVILMLFTGLISLGRLGVDLFPDITFPL
                     VTVRTIYPGAAPAEIENLVTKPIEDELVGLSGLKRISSRSLEGVSFIFVEFTLGTDIK
                     FAVQEVRNKVSLARPNLPETVEEPIIDRFDPLAVPVVRLAVMGDLPPAKLYDITKEEI
                     KTAIEQVADVGNVTIIGGSRREIQVELDRDRMALYQISALSIANKIKSSGTNIPSGKY
                     EKRETETVFRTIGQFEKLSQIENIPISFAGDFGGGVALKTIANVRDGVEDETTRGYLY
                     APVGSPPKNSSVKRELKPAILLDVRKQSGTNTVA"
     gene            1274..1816
                     /locus_tag="PKJ16_13545"
     CDS             1274..1816
                     /locus_tag="PKJ16_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48062.1"
                     /translation="MTVSTVLRVRCMGLIRWGVVVVAAAVLVPICLPGAHGRVLADGA
                     PIVYTLPAGWTQSDEQAAVAAATSPDQSIRIIVRYFPLPSPMTAMQVIQKQKTKVKHD
                     RVVTPPTDMSQLKARFNADSVAKMQLSGVTTEGGKFLYRAFAFVKGRNLAVIEAIVNT
                     KAGEAVFKQADGTVNSFRFK"
     gene            1893..2939
                     /locus_tag="PKJ16_13550"
     CDS             1893..2939
                     /locus_tag="PKJ16_13550"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNV48063.1"
                     /translation="MKRIIQSLICLAVLGTVAADAQAPAPEVRKPVKTSKPGAVGTAL
                     VVFSNDGKYIATAPDGDGVFRLWDAKTLSVKKALGDVKNVLKYAIAFSPDSSLVAAGV
                     GDEIHVWETATYTLVKKFTAHKNARIQSLGFTPDGRFLVSAGSDTDPGKRGCVWSTDT
                     WSLKHHLGYDYGHLWLRSASISPDGRFVVVGGGSDASKDHHALVYDIRSGQKVRELAV
                     FIKANDKFQDGMINNVAYSPSGGQIAVITQSRILGFDAESGEQQFRINKSFHISFQYL
                     RYSPDGKYCAALHKAGRDYCVHIIDARSGSIVRTFVASAGDKRYYYNLAFTHDGKLLA
                     LVGTKATIDFYEWR"
     gene            3022..3276
                     /locus_tag="PKJ16_13555"
     CDS             3022..3276
                     /locus_tag="PKJ16_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48064.1"
                     /translation="MNTNVLLFILFFVGCIGFGVLWEIFRRRCPKCGKFFAREIVASA
                     SSHRHDHRHDRAMKTTVSDCRCKYCGHAWTQKYTSSKRRG"
     gene            3304..4227
                     /locus_tag="PKJ16_13560"
     CDS             3304..4227
                     /locus_tag="PKJ16_13560"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HNV48065.1"
                     /translation="MKNTNDCNFELKQLRSFLVVLEEESFTKASRRLRLGQATISNHI
                     SGLETALGARLIARSSKGFAITEEGALFKKYCERVFGDLDAVRQRMGVQDTPRALTVA
                     ASTVPSQYLLPAALPLLYETFPDLSIHVEVADSREVIESVREGSADIGISGKVVRHRS
                     LAYREICAERIVLASSRRPARAPITPAALRELPFVFREKGSGTRDSYELPLNRLGVHP
                     ADLRPVLTCTTTECVKQAVLAGVGVAFLSELSIRDELRRGALHIHPVSRLSIRRAFHF
                     LWLTSHRHGAFLRRVADTLSVAMREAAARQG"
     gene            complement(4247..5407)
                     /locus_tag="PKJ16_13565"
     CDS             complement(4247..5407)
                     /locus_tag="PKJ16_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HNV48066.1"
                     /translation="MYFDNAATSFPKPAAVIEAMTGFMARGGGNPGRSGHGKSVEAGM
                     EVFRAREALASLMGVNNPMRVVFGHNATDALNLAIQGLARKGDHAVTTSMEHNSVIRP
                     LRELEKRGLISLTVVPCSPEGAADPDAIARAITAGTCLVAANHASNVSGIVQPIGEIG
                     AACRARGVPFIVDASQSAGIVPIDMARDGIDLLAVTGHKALYGPTGTGALIIADSFDY
                     SLLRPLRYGGTGSRSDSIEHPDFLPDRFEAGTLNTVGIAGLLAGIEYLMASGGPKRAG
                     EHKRALTGRFVDAARGSVPGFRPVHPELSQTGVVSFTIDGFSPSEVGEYLDERCVQSR
                     VGLHCAPLAHQTLGTFPAGTVRFSFGMFNTLDEIDEAAGMLADLAAGPARPR"
     gene            complement(5410..5619)
                     /locus_tag="PKJ16_13570"
     CDS             complement(5410..5619)
                     /locus_tag="PKJ16_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV48067.1"
                     /translation="MAQQIDARGLSCPQPVVLTQQAIADGERSITVTVSSSVSKENVI
                     RILERNGYTTDSRDDGDDIVVSGNK"
     gene            complement(5647..6726)
                     /gene="yedE"
                     /locus_tag="PKJ16_13575"
     CDS             complement(5647..6726)
                     /gene="yedE"
                     /locus_tag="PKJ16_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HNV48068.1"
                     /translation="MKNILSTRWGIILAGALIGTIAVSLQFLGNPPNMGVCVACFERD
                     IAGALGLHRADVVQYLRPEIMGFVIGAFFISLALGDYKPRGGSSTLIRFFLGVFAMCG
                     ALVFLGCPWRALLRLAGGDLNALLGIAGLIVGIAIGVVFLKKGFSLGRAYPLKKAAGW
                     IFPAVMLGLFLLLAFQVSFSEGGPVFFSQKGPGSQKANLYISLAAGLVIGAIAQRSRF
                     CTMGSLRDIILTRDTHLVSGIGSLLVFAFIFNLIYGQFNPGFEGQPIAHTDHLWNFLG
                     MTLSGLAYALAGGCPGRQLILSGEGDNDAAVFVTGMIVGAGIAHNFSAASSPKGVGAF
                     GPAMVVIGIVFCLIVGFTMRDRMKA"
     gene            6939..7148
                     /locus_tag="PKJ16_13580"
     CDS             6939..7148
                     /locus_tag="PKJ16_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HNV48069.1"
                     /translation="MKYGAQNKIMAKVKSVKTGDIMSQVKFDVSVPVVMSSVLTTDSV
                     KDLDLKAGDSVQLIIKAIHVLPVKE"
     gene            7194..>7542
                     /locus_tag="PKJ16_13585"
     CDS             7194..>7542
                     /locus_tag="PKJ16_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HNV48070.1"
                     /translation="MSTVIDARGLSCPQPVINAKRALEGADSIVVIVDNRTAVENVSR
                     MAASQGCAVDVEDKGDGIYLFIRKSSGGQPIAQMTPVECGTVNPAGPTVMVFASDTMG
                     CGNDELGGVLMRSL"
BASE COUNT         1469 a         2257 c         2423 g         1393 t
ORIGIN      
        1 gcgacggtgt tggtgcccga ctgcttgcgc acgtcgagga ggatcgccgg cttcagctcg
       61 cgcttcaccg agctgttctt ggggggcgat ccgacgggcg cgtagaggta gccgcgcgtg
      121 gtctcgtcct cgacgccgtc gcgcacgttc gcgatggtct tgagcgcaac gccgccgccg
      181 aaatcgccgg cgaaggagat ggggatgttc tcgatctgcg agagcttctc gaactggccg
      241 atggtgcgga acaccgtctc cgtctcgcgc ttctcgtact tgccgctggg gatgttcgtg
      301 cccgagcttt tgatcttgtt ggcgatggag agcgccgaaa tctgatagag cgccatccgg
      361 tcgcgatcga gctccacctg gatctcgcgc cgactcccgc cgatgatggt gacgttcccg
      421 acgtccgcca cctgctcgat ggcggtcttg atctcctcct tggtgatatc gtagagcttc
      481 gccggcggca agtcgcccat caccgccagg cgcaccaccg gcaccgccag cgggtcgaac
      541 cggtcgatga tgggctcctc gaccgtttcc ggcaggttgg ggcgcgcgag cgagaccttg
      601 ttgcggacct cctgcacggc gaacttgatg tcggtgccca gggtgaattc cacgaagatg
      661 aaggacacgc cctccagact ccgcgagctg atccgcttga gcccggagag gcccaccagc
      721 tcgtcttcga tgggcttggt caccaagttc tcgatctcgg ccggggccgc gccgggatag
      781 atcgtgcgca cggtcaccag cgggaaggtg atgtccggga agaggtccac ccccagccgg
      841 ccgaggctaa tgagtccggt gaagagcatc aggatcacga tgctcatgat aaagatgggg
      901 cgcttgatgg agaaggcggc tatgttcatg tgtcgctcga atcgctggag ttttcgtcgg
      961 atgtccggtc cggcatcagc gcagcgcgcc ggaccgtgcg gctctgggaa ccacggtatg
     1021 gcgaaagtga aaaattaatc aatcaattaa aatgtcgccg gcatgatttt tcccgcaatc
     1081 cagtgaaggc agcatgtgta tgcaccacga attgagggcc tcatgacctg acctgggaaa
     1141 aagttgttgt aaataccgcg aggacggact acggttatcg cgcatcgccg gcatgccgga
     1201 atgcgtcggt cggctttgtg cggccggggt caaacataat acaatatcca tgaacaacgc
     1261 aaaggagggt cacatgacgg tttcaacagt tctccgtgtt cgctgtatgg gtttgattcg
     1321 ctggggcgtg gtggtcgttg cggcggccgt cctcgtgccg atatgccttc cgggagcgca
     1381 cggtcgcgtc ctcgcggatg gcgctcccat cgtctacacg ctccccgccg gttggacgca
     1441 gagcgacgag caggccgcgg tcgcggccgc gacatcgccc gaccagtcca tccgcatcat
     1501 cgtcagatat ttcccgcttc cgtccccgat gacggccatg caggtcatcc agaagcagaa
     1561 gaccaaggtc aagcacgacc gggtggtcac gccgcccacg gacatgtcgc agttaaaggc
     1621 gcgctttaac gccgattccg tcgcgaagat gcaactgtcg ggtgttacaa ccgagggcgg
     1681 gaagttcctc tatcgcgcat tcgcgttcgt caaggggaga aatctcgcgg tcatcgaggc
     1741 gatcgtcaat acgaaggcgg gcgaggcggt tttcaagcag gcggacggga ccgtcaattc
     1801 gtttcgcttc aaatagcgct cgatgcagtg cgcgccgccg gccgatatct cgccggaggc
     1861 gcgtataaaa aatcagaaat gaggacagtg ccatgaaacg aatcatacaa agtctgatat
     1921 gcctcgccgt gctcggcacg gtcgcggcgg atgcgcaggc gccggcgccg gaggtccgca
     1981 aaccggtcaa gacctcgaag ccgggggcgg tcggcaccgc gcttgtcgtt ttttcgaatg
     2041 atgggaaata catcgccacg gccccggatg gcgacggcgt gtttcgactg tgggatgcaa
     2101 aaaccctttc ggtcaagaag gcgctcgggg acgtcaagaa cgtactcaag tacgcgatcg
     2161 cgttttcacc cgacagctcg ctggtggcgg cgggggtcgg ggacgagatc catgtgtggg
     2221 aaaccgcgac atacacgctg gtgaaaaaat tcaccgccca caagaatgcg cgcattcaat
     2281 cactcggctt caccccggac ggccgcttcc tcgtgagcgc cgggtccgac accgatccgg
     2341 gcaaacgcgg ctgcgtctgg agcacggaca cctggtcgct caagcatcat ctcgggtatg
     2401 attacgggca cctgtggctc aggagcgcgt cgatctcgcc ggacggccga ttcgtcgtcg
     2461 tgggcggggg gagcgacgcc tcgaaggacc atcacgcgct cgtctatgat atccgctcgg
     2521 ggcaaaaggt gcgcgagctg gccgtgttca tcaaggcgaa tgacaagttc caggacggca
     2581 tgatcaacaa tgtggcgtat tcgccatccg gcggccaaat cgccgtgatt acccaaagcc
     2641 ggatacttgg attcgacgcg gagagcggcg agcagcaatt tcgtatcaat aagtcattcc
     2701 atatctcctt ccagtatctg cgctactcgc ccgacgggaa atattgtgcg gcgctccata
     2761 aggccggcag ggactattgt gtgcacatta tcgacgcgcg gtcggggtcg atcgttcgga
     2821 cctttgtcgc gtccgccggc gacaagcggt attactacaa cctcgcgttc acacacgacg
     2881 ggaaactgct tgcgctcgta gggacgaagg cgacgatcga cttttatgag tggcggtagc
     2941 atctcgaagc ctgaccggcg tgcggtatcg ttccgcgtgc cggtgggccc gcaggccgat
     3001 tgctagaata ggagacagca catgaatacg aatgttttac tcttcattct tttcttcgtc
     3061 gggtgcatcg ggtttggcgt cctgtgggag atattccgga ggcgttgccc gaaatgcgga
     3121 aaattcttcg cccgggagat cgttgcgagc gcctcgtccc atcggcacga ccaccgtcac
     3181 gaccgcgcga tgaaaacgac ggtgagtgac tgtcgctgca aatactgcgg tcatgcctgg
     3241 acccagaagt acacctcctc gaagcggcgc ggatagcgtc aatccgggga attgaatggt
     3301 gcaatgaaga acacaaacga ctgcaatttc gaactcaagc agctgcgatc cttcctcgtc
     3361 gtgctggagg aggagagctt cacgaaagcc tcgcggcgac tccgcctggg acaggcgacc
     3421 atcagcaatc acatctccgg gctcgagacg gcgctgggcg cgcgactcat cgcgcgctcc
     3481 agcaagggtt tcgcgatcac cgaggaggga gcgctcttca agaaatactg cgagcgcgtg
     3541 ttcggagacc tcgatgcggt gcggcagcgg atgggcgtgc aggatacgcc ccgcgcgctc
     3601 accgtcgccg cgagcaccgt tccctcgcag tatctgctcc ccgcggcgtt gccgctgctc
     3661 tatgaaacat ttcccgatct gagcatacac gtggaggtgg ccgacagccg ggaggtgatc
     3721 gaatcggtgc gcgagggctc cgcggacatc ggcatttcgg gcaaggtggt ccgccatcgg
     3781 tccctggcgt atcgcgagat atgcgcggag cggatcgtgc tcgcctcgtc gcgccgtccg
     3841 gcacgggcgc ccatcacccc cgcggcgctg cgggaacttc cgttcgtgtt ccgcgaaaag
     3901 gggtcgggca cgcgcgattc ctacgagctc ccgctcaacc ggctgggggt gcatccggcg
     3961 gaccttcgac cggtgctcac ctgcacgacg accgagtgcg tgaagcaggc ggtcctcgcc
     4021 ggcgtgggcg tcgcattcct gtccgagctc tccattcgcg acgagcttcg ccgcggcgcg
     4081 ctccacatcc acccggtttc aagactctcc attcggcggg cctttcattt tctgtggctt
     4141 acgtcgcacc ggcacggcgc ctttcttcgc cgggtggccg acacgctgtc cgttgcaatg
     4201 cgggaggccg cggctcggca gggttgatcc cggcgtgccg ccgtccttac cggggacggg
     4261 ccggtccggc cgcgaggtcc gcgagcatcc ccgccgcttc gtcaatctcg tctaatgtat
     4321 tgaacatacc gaagctgaag cgcaccgttc ctgctgggaa tgtccccagc gtttgatgcg
     4381 caagcggcgc gcagtgcagg cccacgcggg actggacgca tcgctcgtcg agatactcgc
     4441 caacctccga cggagaaaac ccgtcaatcg tgaacgagac cacgccggtt tgagacagtt
     4501 ccggatgcac ggggcgaaag ccgggcaccg aaccgcgtgc cgcatcgaca aaacggcccg
     4561 tgagcgcgcg cttgtgctcg cctgcgcgct tcggtccgcc cgatgccatg aggtattcaa
     4621 tgccagccaa cagccccgcg attccaaccg tattcagcgt tcccgcctcg aaacggtcgg
     4681 ggaggaagtc cgggtgttcg atgctgtcgg agcggctgcc ggtcccgccg tagcggagcg
     4741 gccggaggag agaataatcg aaggaatcgg cgatgataag cgcgccggtg ccggtgggcc
     4801 cgtagagcgc cttgtgtccg gtgacggcga gaaggtcgat cccgtcgcgc gccatgtcga
     4861 tgggaacgat ccccgcggac tgcgaggcgt ccacgatgaa gggaacgccg cgcgcgcggc
     4921 atgcggcgcc aatctcacca atcggctgca cgatgccgct cacgttcgag gcgtggttcg
     4981 ccgccaccag gcaggtgccc gcggttatcg cgcgagcgat cgcgtccggg tccgccgcgc
     5041 cctcgggcga gcaggggacg acggtgagcg aaatcagccc gcgcttttcc agctcgcgca
     5101 ggggccggat gacgctgttg tgctccatgg acgtggtgac ggcgtggtcg cccttgcgcg
     5161 cgagtccctg aatggcgagg ttgagcgcgt cggtcgcatt gtggccaaag acgacgcgca
     5221 tgggattatt cacgcccatg agggacgcga gcgcctcccg cgcgcgaaag acctccatgc
     5281 ccgcctcgac ggattttcca tggcccgagc ggcccgggtt gccgccgccc cgggccatga
     5341 agcccgtcat cgcctcgatg accgccgcgg gcttgggaaa gctcgtggcg gcgttatcga
     5401 aatacatcgc tatttatttc cgctgacgac gatgtcgtcg ccgtcgtcgc gcgaatcggt
     5461 cgtatagccg tttcgctcga gtatgcggat cacgttctct ttcgagaccg acgagctcac
     5521 ggtcacggta atggacctct cgccgtcggc gatcgcctgc tgggtgagca ccaccggttg
     5581 cgggcaggaa aggccgcgcg cgtctatctg ctgtgccata tcaatctcct gtgtacattc
     5641 aaataattac gccttcattc ggtcccgcat ggtgaagccg acgatgaggc aaaagacaat
     5701 tccgatgacg accatcgcgg gcccgaacgc tcccacgccc ttcggcgagc ttgccgcaga
     5761 gaagttgtgc gcgattcccg cgccgacgat catcccggtc acaaagaccg cggcgtcatt
     5821 gtcgccctcg cccgacagga tgagctgtcg tcccgggcag ccgccggcga gcgcataagc
     5881 caagccggag agcgtcattc cgagaaagtt ccacaggtga tcggtatggg cgatcggctg
     5941 cccctcgaag ccgggattga actggccgta gatgagattg aatatgaagg cgaatacgag
     6001 aagcgaaccg atgccgctca ccaggtgcgt gtcgcgcgtg aggatgatgt cccgcagcga
     6061 ccccatggtg cagaagcggc tgcgctgggc gatggcgccg atcactagtc cggcggccag
     6121 gctgatgtac aggttcgcct tctgtgagcc gggccccttc tggctgaaga agaccggtcc
     6181 gccctcggaa aatgaaacct ggaaggcgag caggagaaac agtcccagca taaccgcggg
     6241 gaagatccac ccggcggcct tcttgagggg ataggcgcgc ccgaggctaa atcctttttt
     6301 caggaatacc acgccgatgg cgatgccgac gatgagccct gcgatgccca ggagcgcgtt
     6361 gagatcgccc ccggcgagcc gaaggagagc ccgccacggg cagcccagga agacgagcgc
     6421 cccgcacata gcgaatacgc ccaggaagaa gcggatgagc gtcgatgatc cgccccgcgg
     6481 cttgtagtca ccaagcgcga gcgagatgaa aaatgccccg atcacgaagc ccatgatctc
     6541 cgggcggaga tactggacca cgtcggcccg gtggaggccc agcgccccgg cgatatcgcg
     6601 ctcaaaacag gcgacacaca cgcccatatt gggcgggttt cccaaaaatt gcagcgagac
     6661 ggcgatcgtg ccgatgaggg ccccggcaag gatgatgccc catcgggtgg atagaatgtt
     6721 tttcatacgg tctccgtgcg atagtgatga tggtgcatga aacatgccgg gtattgggcg
     6781 ggtcaataat taattttatt aatatatgta attattaatt ttgatattta cagttgacag
     6841 gcgcgttatg gccgctgtat gatgattgtg gtgtttgacg acactccatt cggcgtccat
     6901 gccggaaacc ggcacatcaa tcatgggagg tggcatccat gaagtatggc gcacagaata
     6961 aaatcatggc gaaggtgaaa tccgtcaaga ccggcgacat catgtcgcag gtgaagttcg
     7021 acgtaagcgt ccccgtcgtg atgagctcgg tgctcacgac cgattcggtc aaggacctcg
     7081 accttaaggc gggggattcg gtccagctca tcatcaaggc gatccacgta ttgccggtca
     7141 aggagtagcg cggacgcgat ccgcaatcat cacccgtaca aggagcggaa ggcatgagta
     7201 ccgtaatcga tgcgcgggga ctgtcgtgcc cgcagccggt catcaacgcg aaacgggcgc
     7261 tggaaggcgc cgattcgatc gtcgtgatcg tggacaaccg caccgcggtg gagaacgtga
     7321 gccggatggc ggcatcacag ggatgcgcgg tggacgtcga ggacaaaggc gacggcatct
     7381 atctgttcat acggaaatcc tcaggcggac agccgatcgc gcagatgacg cctgtcgaat
     7441 gcggcaccgt gaacccggcc ggacccacgg tgatggtgtt cgccagcgat acgatggggt
     7501 gcggcaacga cgagctgggc ggcgttctca tgcgcagcct gg
//
LOCUS       DAORHF010000182         7539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9426_length_7539_cov_4.435061, whole genome shotgun sequence.
ACCESSION   DAORHF010000182 DAORHF010000000
VERSION     DAORHF010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9426_length_7539_cov_4.435061"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKJ16_13590"
     CDS             complement(<1..246)
                     /locus_tag="PKJ16_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48071.1"
                     /translation="MEQFLDTTLSFVLRFWDYVRNVVDIVIVAFVFYWLYSFLSNTRA
                     IQLIKGFFIIVIVAGLALVLKFETVTWLIANFATYVVI"
     gene            complement(271..1104)
                     /gene="folP"
                     /locus_tag="PKJ16_13595"
     CDS             complement(271..1104)
                     /gene="folP"
                     /locus_tag="PKJ16_13595"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HNV48072.1"
                     /translation="MRADLGTRTLIMGILNVTPDSFYDGGKYHDNAAAVSRAVDMVDA
                     GADIIDIGGESSRPGARPVSSHEECDRVCPVIEALRNRVTVPISVDTYKAEVARAAIS
                     AGASMINDISGLRSDPSIASIAADAGANLVLMHMRGTPATMQHNTHYDDLITEIAESL
                     AESARIAQEHGVAREKIIVDPGIGFGKTLDDNYKLINNITYFRRLGYPVLIGLSRKSL
                     IGGLLQDGEDRLPGTIALNSIAAYFGADIVRVHDVHEHRLALRMIDHARSLAASDDAA
                     R"
     gene            complement(1104..1982)
                     /locus_tag="PKJ16_13600"
     CDS             complement(1104..1982)
                     /locus_tag="PKJ16_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48073.1"
                     /translation="MPDAIINDVYSIYWNPAGLADLPTTSFASKRGSDDRLAKESYAT
                     PPMNDLTSFSDGETRENTFEVGLSGAGLNLNGGAGFAGIAIRLFSGVMGIGGYGFEAR
                     RIGLYDDDEQLLRKIDYTASAAQCSYGWKMGVTTMGVSLKYLREQAATDHYETGSADI
                     GLQAEVFPLIRLAMVLKDLGPGFRHIEGVEELDDVRAFGAPGIRLSAAVTLPTIDASI
                     VFGAGRPFAIPEYDIGAGIEYQICDVVAVAIGYSTHFVTGSLLFRIGGCSLSYAYAHD
                     PVNHGHNHVVSASGGF"
     gene            complement(2120..2650)
                     /locus_tag="PKJ16_13605"
     CDS             complement(2120..2650)
                     /locus_tag="PKJ16_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV48074.1"
                     /translation="MKTDPAPAVFFDRDGTLNIDTGYIRDPESLRLFDGVPELIARVR
                     ARGYRVFVISNQSGLGRGLIRPDEYRNVSRRFLELCGEGSIDEVVYCPHDPSCECECR
                     KPQGALVDVLAERFAIDMKRSFFIGDKLSDIACGKRFAMRTIFITHGTGVFPSSAEET
                     PDYQVKSIAEIDALLP"
     gene            complement(2654..3475)
                     /locus_tag="PKJ16_13610"
     CDS             complement(2654..3475)
                     /locus_tag="PKJ16_13610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HNV48075.1"
                     /translation="MQRATRLDVHLSTHGLSKSRESAKKEIMAGWVSVDGATIRTPST
                     MIAGTEAISISRPKGVYASRGGDKLHHALTAAQFSVAGVEAVDLGASTGGFTDCLLAH
                     GATRVFAIDVGYGQFEYRLRIDQRVVLMERTNVRTLTRDMFNDANIRVLVADLSFISL
                     TKIFPVIHDVFSPIEGFLLVKPQFEATKGEHKKGVVRGRDRHQAILKRFLDSAVANGI
                     TPRSIIPSPIKGPAGNIEFFVHFVIGNTFEPTETTEIETMVECAIAEAYERFEAE"
     gene            complement(3485..5356)
                     /gene="dxs"
                     /locus_tag="PKJ16_13615"
     CDS             complement(3485..5356)
                     /gene="dxs"
                     /locus_tag="PKJ16_13615"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HNV48076.1"
                     /translation="MLLSSLHSVHDLRRLSPDKLAQLADEVRDYIIEVVSRNGGHLAS
                     SLGVVELTITLHYVFNTPRDKIIWDVGHQCYAHKIITGRKDRFASLRTYGGISGFPKR
                     DESEFDAFNTGHSSTSLSLAVGEAVARDFKKQRYDIIGVIGDGSLTGGMAFEALNHIG
                     HLRKNVIIVINDNEHSISKNVGALSEYLMKLITGSLYNRLRRRSHTIIKRIPRYGNAL
                     YEFLYKMEASLKGILLPGHFFEALGIRYFGPVDGHNIELMIDLFRRIREINSGPRIVH
                     VITKKGKGYEHAEKDPARFHGIGPFDIKSGKSIATGGISFSAVAGKTLASIARTDKNI
                     VAITAAMKLGTGLYEFEKIAPRRFFDVGIAEQHALTFAAAMASKGMKPFVSIYSTFLQ
                     RAIDQLIHDVAIMRLPVRLLIDRAGIVGDDGETHHGIFDIAILKNVPNMTILAPSDAL
                     ELRDMIYYAVRHDSGPLAIRFPRGKTDDASIAFGKHNRFTPGAAHKLTQGNDVAIFAV
                     GDMVSRALAVHDTLKSHRMKSTVINLLSIKPLDSTTITQIVRQTRHFITMENGIMIGG
                     VGEMISAMLPCALASKRLFSVGFPDAFVTHGKNEDLFTLHGMDVDSIVTRLLRACGK"
     gene            complement(5368..6147)
                     /locus_tag="PKJ16_13620"
     CDS             complement(5368..6147)
                     /locus_tag="PKJ16_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HNV48077.1"
                     /translation="MSDTIAVLAIGDIVGSPGRDLIERNLKALVARHNVDFVIANGEN
                     ASGGHSLQPKNASELLSYGVDVITSGNHIWHSRKILEIIDIEPRLLRPANYPQGAPGR
                     GYCITRVGETPVCVINLIGRIELAMVDCPFRKFDAIYNEVRERARIIIVDFHAEATSE
                     KRAFGWYCDGRASVVFGTHTHVQTADDEVLTKGTGYISDVGMTGSFNSVIGVDKEKSI
                     QNFLTQTRVPFDVAGGNEKINGAVFTIDRQGKTINIVRINE"
     gene            complement(6162..>7539)
                     /gene="rny"
                     /locus_tag="PKJ16_13625"
     CDS             complement(6162..>7539)
                     /gene="rny"
                     /locus_tag="PKJ16_13625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HNV48078.1"
                     /translation="ARRVIEAKDQILKEKNGFEKEMRDRRQELQNIEKRIIQKEEACD
                     QRNGQLDKQEKVLKAREDEIVEKEDELRKEFESHRKELERISGLSTDEAKKMLLKNLE
                     NEIRFDSTRLINKIEEEARRTADKKAKEVVLTAIQRNASDFTSESTITTVPLPSDDMK
                     GRIIGREGRNIRTLENLTGVDMIIDDTPEVVVISGFDPVRREIARQSLERLIHDGRIH
                     PARIEEVVEKVRNEIEANMLEEGEKSSFELGIPGMSKDALYAIGKLKYRSSYGQNVLS
                     HSKEVANLAGIMAGELKLDVLLAKRSGLLHDIGKGSMAEGEGAHALVGADMAKKFGEN
                     DVVVNVIASHHNDKEPESFEATLIQVADAISASRPGARKESLDTYLKRLDNLEHIAMS
                     FKGVEKCYAIQAGREIRVLVANDVISDDRAESLAREIAHKIETELKYPGIVRVTVIRE
                     TRVVDYAK"
BASE COUNT         1543 a         2208 c         2125 g         1663 t
ORIGIN      
        1 tatgacgaca taggtggcga aattcgcgat cagccaggtc acggtctcga atttgaggac
       61 cagcgccaaa cccgccacga taacgataat aaaaaatccc tttatgagct ggattgcgcg
      121 ggtattggac agaaacgagt agagccaata gaacacaaag gcgacgatga cgatgtcgac
      181 cacgttgcga acatagtccc aaaacctgag gacgaatgag agcgtcgtat cgagaaattg
      241 ttccatgttt gttctatatc gtctctgtcg tcatcgtgcc gcatcatccg aggcggccag
      301 ggatcgagcg tgatcgatca tccgtagcgc caggcgatgt tcgtggacat catgcacgcg
      361 cacgatatcc gccccgaaat atgccgcaat actgttgagc gcgattgtgc ccgggagccg
      421 gtcctcgccg tcctgcagca gcccgccgat aagggacttc cgggacaggc caatcaggac
      481 cggatagccg agacgccgaa aatatgtgat attgttgatt agtttgtaat tatcatcaag
      541 tgtttttccg aatccgatgc ccggatcgac gattattttt tcacgggcca ccccatgctc
      601 ctgagcgatt cgtgccgatt ccgccagcga ttctgcgatc tcggtgatca gatcgtcata
      661 gtgcgtattg tgctgcattg tggcgggcgt cccccgcatg tgcatgagga cgagatttgc
      721 gccggcatcg gcagcgatcg aggcgattga cggatcggac cgcagcccgc ttatatcgtt
      781 gatcatgctg gccccggccg atatcgccgc gcgggcaacc tctgctttat atgtgtcgac
      841 ggaaatgggt acggttacac gatttcgcag cgcctcgatg accgggcata cgcgatcgca
      901 ctcctcatgt gacgagaccg gccgggcccc cggccgtgac gattctccgc cgatatcaat
      961 gatgtccgct cccgcgtcga ccatgtcgac tgcacgggaa acagcggccg cattatcgtg
     1021 gtatttcccg ccgtcgtaga atgaatccgg cgttacgttg aggatgccca taatgagggt
     1081 gcgcgttccc aggtccgcac gcatcagaag ccccctgagg cagaaacgac atgattatgc
     1141 ccgtgattca ccggatcgtg ggcatatgcg tacgatagag agcatcctcc aatacgaaag
     1201 agcagcgagc cggtcacgaa atgcgtgcta taaccgatgg cgacggcgac gacatcacag
     1261 atctgatatt caataccggc cccgatatcg tattcgggaa tcgcgaaggg ccgccccgcg
     1321 ccaaagacaa tgctcgcgtc aatcgtcgga agcgtgaccg cggcgctgag gcggataccc
     1381 ggcgcgccga atgcgcgaac atcgtcgagc tcttcgacgc cctcgatgtg acggaacccc
     1441 ggcccaagat ctttgaggac catcgccaac cggatcagcg gaaaaacctc ggcctgcagc
     1501 ccgatatccg cgctgccggt ctcataatgg tcggttgcgg cctgctcgcg cagatacttc
     1561 agcgagacgc ccatcgttgt gacgcccatc ttccaaccat aggagcactg ggcggctgat
     1621 gcggtataat cgatcttccg caacagctgt tcgtcatcat cgtagagccc gatccgtcgc
     1681 gcctcgaatc catagccgcc gatccccatc acccccgaaa aaagacgaat cgcgattccg
     1741 gcaaaacccg caccgccatt cagattgaga ccggcgccgc tcaggccgac ttcaaaggtg
     1801 ttttcgcgcg tttcaccatc ggaaaaggat gtgagatcgt tcatcggtgg agtcgcgtac
     1861 gattccttcg caagtcgatc gtcagaaccg cgtttcgacg caaacgatgt tgtgggaaga
     1921 tccgcaaggc ccgccggatt ccagtatatc gaatacacat cattgatgat cgcatcgggc
     1981 atcctgccga gtccgaccgc gcgggcgcca acccatccgt tcgcatacga ggcgttgagt
     2041 ttggtcgcct gccgcgcctc gagcgcctgc agttgcgcaa tcacggtgat tccgattgtg
     2101 agaagaactg cgaatcggtt catggcagta gtgcgtcaat ctccgcgatg gatttcacct
     2161 gatagtccgg ggtctcctcc gcagaggacg gaaacacccc agtgccgtgt gttataaaaa
     2221 ttgttcgcat cgcaaaacgt tttccgcagg cgatatcgga cagcttgtcg ccaatgaaaa
     2281 aggaccgctt catgtcgatt gcgaaccgct ccgcaaggac gtccaccaat gcgccctgag
     2341 gctttcggca ttcgcactcg catgatggat catgcgggca gtatacgact tcgtcgatac
     2401 taccttcgcc gcataattcg agaaaacggc ggctcacgtt acgatactca tccggcctga
     2461 tgagcccgcg gccgagtccg gactgattcg agatgacgaa cacgcggtag cctcgcgcgc
     2521 gaacccgcgc gatgagctcg ggaaccccgt cgaacaagcg cagagactcg ggatcccgta
     2581 tgtacccggt atcgatattg agggttccat cgcgatcgaa gaaaacggcg ggtgccggat
     2641 cggttttcat cgattattcc gcctcgaatc gttcataggc ttctgcgata gcgcactcga
     2701 ccattgtctc gatctctgtc gtttcagtgg gttcgaacgt gttgccaatg acgaagtgga
     2761 cgaagaattc aatattaccg gccggtccct ttattggcga cggtataatc gaccggggcg
     2821 tgatgccgtt cgcaactgcg ctatcgagaa aacgcttgag aatcgcttgg tggcgatctc
     2881 gtcccctcac gacccctttt ttatgctcgc cctttgtcgc ctcgaactga ggtttaacga
     2941 gcaggaagcc ctcgatcgga gaaaacacat cgtgaataac cgggaagatt ttcgtgagtg
     3001 agataaacga caaatcggcg acaagaacac ggatgttggc gtcattgaac atgtcgcggg
     3061 tgagggttcg aacattcgtc cgctccatga gcacgacgcg ctggtctatt cgaagacgat
     3121 attcgaactg tccgtagccg acatcgatcg cgaaaaccct ggtcgcgccg tgtgcgagca
     3181 ggcaatcggt aaagccgccc gttgaagcgc caaggtcaac cgcttcaact cccgcaaccg
     3241 agaactgcgc ggcagtgagc gcatgatgca atttgtcacc gccccggctc gcgtacacgc
     3301 ctttcgggcg cgaaatcgaa atcgcctctg tgcccgcgat catcgtcgac ggcgtccgta
     3361 tcgttgcgcc atcgacggag acccatcccg ccattatctc ctttttcgcg gactcccgtg
     3421 atttcgaaag tccgtgcgta gagagatgca cgtcgagacg ggtagcgcgc tgcatcacca
     3481 ccgctcactt gccacacgcg cgaagaaggc gcgtgacgat cgaatcgaca tccatgccgt
     3541 gcagcgtgaa gaggtcctcg tttttcccgt gcgtaacgaa tgcgtctgga aacccgaccg
     3601 aaaaaagccg cttcgaggca agcgcgcacg gcagcatggc ggatatcatt tcaccaacgc
     3661 cgccaatcat gattccgttt tccatggtga taaaatggcg ggtttgccga acaatctgcg
     3721 tgatagtcgt actatcgagc ggtttgatgc tcagaagatt tatgacggtt gatttcatcc
     3781 gatgagactt gagcgtgtcg tgtaccgcaa gcgcgcggct gaccatgtcg ccaacagcga
     3841 aaatcgctac atcgtttccc tgcgtgagct tgtgcgccgc accgggcgta aaacgattgt
     3901 gcttgccgaa ggcaattgag gcgtcgtcgg ttttgccgcg ggggaaccgt atcgccaggg
     3961 gaccggagtc atggcgcacc gcatagtata tcatgtcgcg taattcgagg gcgtcagacg
     4021 gcgcgaggat ggtcatgttc ggaacgtttt tcaagatcgc tatatcgaaa atgccgtgat
     4081 gcgtctcgcc gtcgtctccg acgatgccgg cccggtcgat gagcaatcgg accggaagcc
     4141 gcatgatggc gacatcgtga atgagctgat cgatggcgcg ctgcagaaag gtcgaataga
     4201 ttgacacgaa cggcttcatc cctttcgacg ccatcgccgc ggcgaatgtc agtgcgtgct
     4261 gctccgcgat tcccacatcg aaaaaccgtc gaggtgcgat tttctcgaac tcgtacagcc
     4321 cggttccgag cttcatcgcg gcggtaatgg ccacgatgtt cttgtccgtg cgggctatcg
     4381 aagcgagcgt ttttccggcg acagccgaaa atgaaatccc gcctgtggcg atcgatttcc
     4441 cgctcttgat gtcgaaggga ccgatgccgt gaaatcgggc cgggtctttt tcggcgtgct
     4501 cgtatccctt tcccttcttc gttattacat gcacaatgcg cggcccagag ttgatttcgc
     4561 gaattcgccg aaaaagatcg atcatgagct cgatattgtg tccatcgacc ggaccgaagt
     4621 agcgaatccc cagcgcctcg aaaaaatgac cgggcaacag gatgcccttg aggctcgctt
     4681 ccattttgta caagaactcg tagagcgcat tgccgtagcg ggggatgcgt ttgatgatcg
     4741 tatgggagcg acgccgcaac cggttgtaga gggaaccggt tatgagcttc atgagatact
     4801 cggacagcgc accgacattt tttgatatgg agtgctcgtt gtcattaatg acgatgatga
     4861 cgtttttccg aaggtggccg atgtggttga gcgcctcgaa ggccatgccg ccggtcaggg
     4921 agccgtcgcc gatgacgccg atgatgtcgt atcgctgctt cttgaaatcg cgcgcgacgg
     4981 cctcgcccac ggcgagcgag agactggtgc tgctgtgccc cgtattgaac gcgtcgaact
     5041 ctgattcgtc gcgcttggga aaaccgctga tgccgccata ggtgcgcaga gaggcgaaac
     5101 ggtccttgcg tccggtgatg atcttatgcg catagcactg atggccgacg tcccagatga
     5161 tcttgtcgcg cggggtattg aatacgtagt gaagcgtgat cgtcaattcg acaacgccca
     5221 gtgacgacgc aagatggccg ccgtttcgag aaacgacctc aatgatgtaa tcacgcacct
     5281 cgtctgcaag ctgcgcgagc ttatccggcg agagacgtcg aagatcatgt actgaatgta
     5341 acgagcttaa caacacagaa cgcaccgtta ttcgtttatt cgaacaatat tgatcgtctt
     5401 cccctgtcgg tcgatcgtga acaccgcgcc attgatcttc tcattcccgc cggcgacatc
     5461 gaacgggacg cgcgtctggg tgagaaagtt ctggatcgat ttttccttgt cgacgccgat
     5521 caccgaattg aacgagcccg tcatgcccac gtcgctgatg tatcccgtgc ccttggtcag
     5581 cacctcgtca tcggccgtct gcacatgcgt atgcgtgccg aagaccacgc tcgcccgccc
     5641 gtcgcaatac cagccgaagg cccgcttctc cgaggtcgcc tctgcgtgaa aatcgacgat
     5701 gatgatccgc gcgcgttctc gcacctcatt gtagatggcg tcgaacttgc ggaaggggca
     5761 atccaccatc gccagctcga tgcgtccgat aaggttgatg acgcataccg gcgtttcgcc
     5821 gacgcgggtg atgcaatacc cgcgacccgg agcgccctgc ggataattcg ccgggcgcag
     5881 cagtcgcggc tcgatgtcga tgatctcaag gatcttccgg ctgtgccaaa tgtggttgcc
     5941 ggaggtgatg acatccacgc cataggagag cagttcgctt gcgttcttcg gctgcagcga
     6001 atgcccgccc gaggcgttct ctccgttggc gatgacgaaa tcaacattgt gccgcgcaac
     6061 caatgctttc agattgcgct cgatgaggtc acgccccggg ctcccaacga tgtcgccgat
     6121 cgcgaggacg gcaatggtgt cactcatgcg cggtccgccg gctacttcgc ataatcgaca
     6181 acccgcgttt cacggatcac ggtaacccgc acgatgccgg gatacttgag ctcggtttcg
     6241 atcttgtgcg cgatttcgcg ggcgagcgat tccgcgcgat cgtcgctgat cacgtcgttt
     6301 gccaccagca cgcgtatctc gcgcccggcc tggatggcgt agcatttctc gacgcctttg
     6361 aaggacattg cgatgtgctc gagattgtcc agtcgcttca ggtaggtgtc gagcgattcc
     6421 ttgcgcgcgc ccggacgcga cgccgagatc gcgtcggcca cctggatgag cgtcgcctcg
     6481 aacgattccg gctccttgtc gttgtggtga gacgcgatca cgtttaccac cacgtcgttt
     6541 tcgccgaatt tcttcgccat gtcggccccc accaacgcgt gggcgccctc cccctcggcc
     6601 atgctcccct ttccgatatc gtgaagcagt ccggagcgct ttgcaagcag gacatcaagc
     6661 ttgagctcgc cggccatgat gcccgcgagg ttcgcgacct ccttgctgtg tgaaaggacg
     6721 ttttgcccgt agctgctgcg gtacttcagc ttcccgattg cgtagagcgc gtccttgctc
     6781 atgccgggaa tgcccagctc gaacgaggat ttttcgccct cctcgagcat gttcgcctcg
     6841 atctcattgc gcaccttctc gaccacctcc tcgatgcgcg cggggtgaat gcgcccatcg
     6901 tgaatcagcc gctcgaggga ctgccgggcg atctcgcgtc gcacgggatc gaaaccggaa
     6961 atcaccacga cctccggcgt gtcgtcgatg atcatgtcca cgccggtcag gttctccagc
     7021 gtgcgaatat tgcgcccctc gcggccgatg atgcgccctt tcatgtcgtc cgaaggcagc
     7081 ggcacggtgg tgatggtgct ctcggacgtg aaatcggacg cattgcgctg tatggccgtg
     7141 aggacgacct ccttggcctt cttgtcggcg gtccggcgcg cctcctcctc gatcttgttg
     7201 atgaggcggg tcgaatcaaa ccgtatttcg ttttcgaggt ttttcaggag catcttcttc
     7261 gcctcgtcgg tggaaaggcc cgaaatccgc tcaagctcct tacgatggct ttcgaattcc
     7321 ttgcgcaatt catcttcctt ttcgacgatc tcgtcttccc tggccttcag caccttctcc
     7381 tgcttgtcga gctggccatt tcgctgatcg caggcctctt ccttctgtat gatccgtttc
     7441 tcgatgttct gcaattcctg ccgacgatcg cgcatctcct tttcgaagcc gttcttctcc
     7501 ttgaggatct ggtccttcgc ctcgatgacg cggcgggcg
//
LOCUS       DAORHF010000183         7497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9507_length_7497_cov_5.153185, whole genome shotgun sequence.
ACCESSION   DAORHF010000183 DAORHF010000000
VERSION     DAORHF010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7497
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9507_length_7497_cov_5.153185"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PKJ16_13630"
     CDS             <1..263
                     /locus_tag="PKJ16_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48079.1"
                     /translation="AVKKDFPKASIYGLKEFDGLRVITILKDAPEKFGLPINPTPINI
                     TKVEEINNLYSFFSLFTFGLPFLKRTAYKVARSMTVGDRGLS"
     gene            414..1088
                     /locus_tag="PKJ16_13635"
     CDS             414..1088
                     /locus_tag="PKJ16_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HNV48080.1"
                     /translation="MGYHVLCVVVSLVIALSAGCSNGAGEAPVCPHAFGGNGGTHGAP
                     HTLIGKTVPNLALDAFHKGEIKTVRLADYRGAWLVLVFYPADFTYVCPTELRELAELY
                     GKFTALKAEVVSVSTDSMFVHRAWHEHNEAVRRVTFPMVSDRAGKLSRALGAYVEAKG
                     AAERASFVVDPEGRIVAYEFHDETIGRSADELLRKLEAAVAVRASNGDFCPAGWRPGR
                     DMVTPR"
     gene            complement(1085..2179)
                     /locus_tag="PKJ16_13640"
     CDS             complement(1085..2179)
                     /locus_tag="PKJ16_13640"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HNV48081.1"
                     /translation="MEPLDLHFMRNDPVEAVMLVDIATRRARANAAFRDLVGTPTEGR
                     FDELITTVSGDHAQQAFLRDEIFTGTFDARRPLDVRTGPRHYRLYAHRDGGVIVIHAR
                     DMAELDRVTEELSEYARGLTENTVGLEMSQREIRRQYEELQEELRKRMMAQDLLHQSI
                     LNLNRIIHEMIATISTISILRDPYTGKHQQRVAHLARSIAEELELPLDQIQTIYVAGM
                     IHDVGKLSIPTEILSKPGKISEVEFLLIQNHPQISYEILKLIEFPWPVATIALQHHEK
                     LDGSGYPNGIRGDEILPETKIITVADTVEAISSHRPYRAGLGIESALEEILIHRGTKY
                     DPAAVDTCARLFREKNFDFEKDETKDLFMA"
     gene            complement(2216..2602)
                     /locus_tag="PKJ16_13645"
     CDS             complement(2216..2602)
                     /locus_tag="PKJ16_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48082.1"
                     /translation="MAHIDIPSFSLDKVTIRVSDHDGVPCVVFSGMMDVRDPSQEVLP
                     YLLSIHQRIISGGIKRIRVDFSDLSFMNSSGIRSVISWVVKLNDLPAESRYRITIVHN
                     PEITWQQSSLKVMQQLLPDHIELEKH"
     gene            complement(2615..3106)
                     /locus_tag="PKJ16_13650"
     CDS             complement(2615..3106)
                     /locus_tag="PKJ16_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48083.1"
                     /translation="MMDTNIGNVAGRKAFVRLQFGPNWNYIATVRIFVMNFLAITLED
                     KKKADVIAMAVNEMVENAIKYSNQACIEIHLDVFEGDKTITVMVGNHATRESYESFTE
                     ILDEISARPPLEAFLERMRVSSSGPDRKSMIGLARIRYESGAKLSHTFEDGYLRVYAS
                     FDS"
     gene            complement(3220..3585)
                     /locus_tag="PKJ16_13655"
     CDS             complement(3220..3585)
                     /locus_tag="PKJ16_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV48084.1"
                     /translation="MRPIFAAAPLCLALAIALGATVAGTAAQKVVLYYFYGTGCVNCA
                     KTQPIVEQLAYKYRTRGLTLQSYEVWYNTNNRALLLRLAQERGKSVQGVPIVIIGRDV
                     YMGVKSISEIERRINTYLK"
     gene            complement(3582..4208)
                     /locus_tag="PKJ16_13660"
     CDS             complement(3582..4208)
                     /locus_tag="PKJ16_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48085.1"
                     /translation="MRKKVVPTQVKNPELVNKRRQQIIDASVRLFIKQGFHKSTTRQI
                     ARKAGFSIGKLYEYITTKEDVLYLVCDYIHRGVEEAVKQAITDSGTGRESLESAIREY
                     FRICDSMSDDILLIYQEIKSLARDAMHTVLARDVQITELFEQIIDRGIRDRSIKLNVP
                     SARLLAHNIMVLGHMWTFRRWYLSRTITIDQYIEEQLQFILNSIGRKP"
     gene            4457..5686
                     /locus_tag="PKJ16_13665"
     CDS             4457..5686
                     /locus_tag="PKJ16_13665"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HNV48086.1"
                     /translation="MESYIQRLFADRIGGSRFGKEEKIYKFEKIKRAKREAVKNNPGT
                     ALIDLGVGEPDEKAFDVVIKTLQSEAENPANRGYADNGIDEFKAAAAAYLKNVFGVGG
                     INPETEVMHAIGSKSALSMFPSAFINPGDVVLMTVPGYPVFGTHAEWYGGAVHNLPLR
                     RENAFLPDLDEIPADVLKRAKVMVLNYPNNPTGANADAGFYRQVVDFANRNGVIIVQD
                     AAYAALTYASKPLSFLNVPGAKDVGIEIHSLSKSFNMTGWRLAFVAGNELIVKAFGNV
                     KDNFDSGQFIAIQKAGVQALAHPEITAAIAAKYERRLKALVAILTRLGFSAAMPAGTF
                     YLYFEIPKGVKGGPSFATAEEFSQFLIRERLISSVPWDDVGHFIRFSATFVATPDEET
                     AILEEIERRLSDLAFVF"
     gene            5896..>7497
                     /locus_tag="PKJ16_13670"
     CDS             5896..>7497
                     /locus_tag="PKJ16_13670"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HNV48087.1"
                     /translation="MSKKKWWIIPAVAFIPGIIVGALALYFYKGLVRYNGVISAHAGE
                     PVTIERDRNGVPAIRAKGATGVFFGIGFVHAQDRLAQIEYFRAIARGRLAELIGEHAV
                     VPDKLARTVGFHRSAVELVKRVKSPAREYLAAYAAGINLYRSEMAHEFMGLLGALPEP
                     WTDADVASILLLFEWTDAFSANRELIMPVADNEHREALMQIVPPVLQYAYGDEERSTV
                     RALKGVRRALAVLVDESPDGFAFYIPGAQSKDGRGYLGVSIAGSIDLFPRHYPLVMES
                     ERGSISGITAAGLPFMTMGAGPALSFAGYTLRCDTQQFYLEKIAVVSGKAMYLSKGAL
                     RDFEASTETIEIKNPGGRRDRIVFTARRTERGPVISDIFSDRMKTEVVSVEALAPDEG
                     LVAALFELPFADSVQKARAVIAGLNANPRAFLFAGSDGVQEALAGRLPVIQPPMPGAV
                     LRDGSAYVVRTVADLSGYVRPITTEFAVLGPEILDAAPPVVKEAMMPRNTHVPARIAE
                     HLDRDMLLDERACDRLFADTTSTLAE"
BASE COUNT         1330 a         2241 c         2405 g         1521 t
ORIGIN      
        1 cggcggtcaa gaaggacttc ccgaaggcca gcatctacgg gctcaaggag ttcgatggat
       61 tgcgggtgat caccatactc aaggacgcgc cggagaaatt cggcctgccg atcaacccca
      121 cgcccatcaa catcacaaag gtcgaggaga tcaataacct ctacagcttc ttctcgctgt
      181 tcacgttcgg tctccccttt ctcaaacgca ccgcgtacaa ggtggcgcgc tcgatgaccg
      241 tgggagaccg cggcctgtcg tagccgacgc ctcatgtcga tgttcaccga acggggaggc
      301 cgaacgcctc cccgttcgtt ttaataatcc cttgaaaacc cgtcccgcat gcgatacgct
      361 tgccgcgaat cgacgtgaaa taaaaaatac gaacgaaacg aaggagcgat cgtatgggct
      421 atcatgttct ctgtgtcgtg gtatcgctgg taatcgcgct ttcagccggc tgttcaaacg
      481 gcgccgggga ggcgccggtg tgtccacatg cattcggtgg gaacggaggg acgcacggcg
      541 cgccgcatac gcttatcggg aaaaccgtgc cgaacctggc gctcgacgcc tttcacaagg
      601 gcgagatcaa aacggtgcgg ctagcggact atcgcggcgc ctggctggtc ctcgtgttct
      661 atcccgccga cttcacctat gtgtgcccca ccgagctcag ggagctcgcc gaactgtacg
      721 gcaagttcac ggcgctcaag gcggaggtgg tgagcgtgag cactgattct atgttcgtgc
      781 acagggcctg gcacgagcac aacgaggccg ttcggcgcgt gaccttcccc atggtttcgg
      841 accgcgccgg gaagttgtcc cgggcgctcg gcgcatatgt cgaggcgaag ggcgccgcgg
      901 aacgcgcgag cttcgtggtg gatcccgagg gccggatcgt cgcctacgag ttccatgacg
      961 agacgatcgg acggagcgcc gatgagctgc ttcgcaagct cgaggcggcg gtagcggtgc
     1021 gcgcgagcaa cggcgacttc tgtccggctg gctggcggcc gggccgggac atggtgacgc
     1081 cccgctaggc cataaagagg tctttggttt cgtccttctc gaagtcgaag ttcttttcgc
     1141 ggaacaggcg cgcgcaggtg tcgaccgccg cggggtcgta tttggtgccg cggtggatca
     1201 ggatctcctc gagcgcgctc tcgatcccca ggcccgcccg gtagggccgg tgggagctta
     1261 tggcttcgac ggtgtcggcc accgtgatga tcttcgtctc gggcaggatc tcgtcccccc
     1321 ggatgccgtt cggatatccc gatccatcga gcttttcatg gtgttgcagg gcgatcgtgg
     1381 ccaccggcca ggggaactcg atgagcttga ggatttcgta ggatatctgc gggtggtttt
     1441 gtatgaggag aaactcgacc tcgctgatct tgccgggctt gctcaggatc tccgtgggga
     1501 tggaaagttt tccgacatca tggatcatgc ccgcgacata gatcgtctgg atctggtcca
     1561 gggggagctc cagctcctcg gcgatcgagc gcgccaggtg ggccacgcgc tgctggtgct
     1621 tgccggtgta gggatcgcgc aggatgctga tggtcgaaat ggtcgcgatc atctcgtgga
     1681 tgatgcggtt gaggttcagg atgctctggt ggagcaggtc ctgcgccatc atgcgcttcc
     1741 gcagctcctc ctgcagctcc tcgtactgcc gccggatctc gcgctggctc atctcgagcc
     1801 ccacggtgtt ctcggtgagg ccgcgcgcgt actcgctgag ctcttcggtc acgcggtcga
     1861 gctcggccat gtcgcgcgcg tggatgacga tgacgccccc gtcgcggtgc gcatagagcc
     1921 ggtagtggcg cgggccggtg cggacgtcga gcggccgccg cgcgtcgaag gtcccggtga
     1981 aaatctcgtc gcgaagaaac gcctgctgcg cgtgatcgcc gcttacggtc gtgatgagct
     2041 catcgaaacg tccctcggta ggggttccca cgaggtcgcg gaaggccgca ttggcgcgag
     2101 cgcgccgcgt tgcgatgtcg accagcatga cggcctccac cggatcgttt cgcatgaaat
     2161 gaaggtcaag tggttccatc gcgtcgtccg gagttgccgg cataagcgct atccgctagt
     2221 gtttctccaa ttcgatgtgg tcggggagga gctgctgcat gaccttgagg ctgctctgtt
     2281 gccaggttat ctcggggttg tggacgatgg tgatgcgata gcgcgattcg gccgggaggt
     2341 cgttgagctt gacgacccag gagatgacgg agcggatgcc gctggagttc atgaacgaca
     2401 gatcggagaa atcgaccctg atgcgcttga tgccgccgct gatgatgcgc tgatggatcg
     2461 agagcaggta cggcaatacc tcctgcgacg ggtcgcggac atccatcatc ccggaaaaga
     2521 ccacgcaggg aacgccgtcg tggtcgctca cgcggatggt caccttgtcg aggctgaagg
     2581 agggaatgtc gatgtgtgcc attgtcgctc ctgtctatga gtcgaaactg gcgtacaccc
     2641 tgagatagcc gtcctcaaag gtgtgcgata gcttcgcgcc cgattcgtac cgtattctcg
     2701 ccaagccgat catgctcttg cggtcgggcc cgctcgatga aacccgcata cgctccagga
     2761 aggcctccag cggcggacgg gcggatatct cgtcgagaat ctccgtgaag ctttcgtacg
     2821 attcgcgcgt cgcgtggttt cccaccatca cggtgatcgt cttgtcgccc tcgaacacgt
     2881 ccaggtgaat ctcgatgcag gcctggttcg aatacttgat ggcgttctcg accatctcgt
     2941 tgaccgccat cgcgattacg tccgcctttt ttttgtcctc gagcgtgatg gccaggaaat
     3001 tcatcacgaa aatcctgacg gtggcgatgt agttccagtt cggcccgaac tggagccgta
     3061 caaacgcctt cctccccgcg acgttcccga tgttcgtgtc catcatatcc gtatccggcc
     3121 gggagaaccc cttcacgcat gccgatcggt cccctccgtc cgcttaaatg tactgagggt
     3181 cgggcaataa ttcaagtatt ttttaaaaac ggtcgcggac tatttcaggt aggtgttgat
     3241 tctccgttct atctcggaga tgctcttgac gcccatgtat acgtcgcggc cgatgatgac
     3301 gatcgggacg ccctgcacgc ttttcccccg ctcctgggcg agtcggagca gcagcgcgcg
     3361 gttgttcgtg ttgtaccaga cctcgtagct ttgaagcgtg agtccgcgcg tgcgatactt
     3421 gtatgcgagc tgttcgacga tcggctgggt tttcgcgcaa ttgacgcacc ccgttccgta
     3481 aaagtaatag agaaccacct tctgtgcggc agtgccggcg actgttgcgc cgagggcgat
     3541 ggcgagcgca aggcacagcg gtgcggctgc gaaaatcggt ctcatggctt cctcccaatg
     3601 ctgttcagga taaactgaag ctgttcttcg atatactggt cgatggtgat ggtccggctg
     3661 agataccacc gacggaaggt ccacatgtgt cccaggacca tgatgttgtg cgccagcagc
     3721 cgcgccgacg ggacattcag cttgatgctc cggtcgcgga tcccccggtc gatgatctgc
     3781 tcgaagagtt ccgtgatctg gacgtcgcgg gcgagcaccg tgtgcatggc gtcgcgggcg
     3841 agcgacttga tctcctggta gatgaggagg atgtcgtcgg acatgctgtc gcagattcgg
     3901 aagtactcgc ggatggcgct ctccagcgat tcccgtccgg tgccggaatc ggtgatggcc
     3961 tgcttgacgg cctcctcgac gccgcggtgg atgtagtcgc agacaaggta gaggacgtcc
     4021 tccttggtgg tgatgtactc gtagagcttc ccgatgctaa agcccgcttt gcgcgcgatt
     4081 tgccgcgtgg tgcttttgtg gaacccctgc ttgatgaaca ggcgcacgct cgcgtcgatg
     4141 atctgctgac ggcgcttgtt caccagctcc gggttcttga cttgtgtagg gacgaccttt
     4201 tttctcatgg ctcccgtcgc gctcgaaggt tgtcggtaat tccgactgaa cgctcgattt
     4261 aatttcgagc cgcccgcagt gtcaagagat aaaaaaacgc gccgacgggc tcattttttt
     4321 ccggcccgcc gaatttgttc ttgtaaaaaa ttgcccgccg cgaaccatat tcaggacaaa
     4381 tcatcgccag tcagtccgca cgcacggccc ggggcagtcg ttacagccat accggcacga
     4441 atacggggta gggaccatgg agtcgtacat tcaacgcctc tttgccgatc gcatcggcgg
     4501 cagccgcttc ggcaaggaag agaagatcta caaattcgaa aaaatcaagc gggccaagcg
     4561 ggaggccgtc aagaacaatc ccggcactgc gctcatcgac ctgggcgtcg gcgagcccga
     4621 cgaaaaggcg ttcgacgtcg tcatcaagac cctccagtcg gaggcggaga accccgccaa
     4681 ccgcggatac gccgacaatg ggatcgacga attcaaggcc gccgcggccg cgtatctgaa
     4741 aaacgttttc ggcgtgggcg ggatcaaccc cgagacggag gtgatgcacg ccatcgggtc
     4801 gaagtcggcc ctgtcgatgt ttccctccgc tttcatcaac cccggcgacg tggtgctgat
     4861 gaccgttccc ggctacccgg tcttcgggac gcacgcggaa tggtacggcg gcgcggtgca
     4921 caacctcccg ctcaggcgcg agaacgcctt tttgcccgac ctggacgaga tccccgccga
     4981 tgtgctgaag cgcgccaagg tgatggtgct caactacccg aacaatccga ccggcgcgaa
     5041 cgccgacgcc gggttttacc ggcaggttgt cgatttcgcg aaccgcaacg gcgtaatcat
     5101 cgtccaggat gccgcgtatg cggcgctcac ctatgcgtcg aaaccgctct cgtttctgaa
     5161 cgtgcccggc gcgaaggacg tgggaatcga gatccactcg ctttcgaaat cgttcaacat
     5221 gaccggctgg cggctcgcct tcgtcgcggg caatgaactc atcgtgaagg ccttcgggaa
     5281 cgtgaaggac aatttcgact ccggccagtt catcgcgatc cagaaggcgg gcgtgcaggc
     5341 gctcgcgcat cccgagatca ccgcggcgat tgcggcaaag tacgagcggc gcttgaaggc
     5401 gctggtggcg attctgacgc gattgggatt ttcggccgcc atgcccgcag ggaccttcta
     5461 tctgtatttt gaaattccga agggcgtgaa gggcggcccc tcgttcgcca ccgccgagga
     5521 gttttcccaa ttcctgatcc gtgagcggct catctcgtcg gtgccctggg acgatgtggg
     5581 acattttatc cgcttctccg cgaccttcgt ggcgaccccg gacgaggaga cggccattct
     5641 ggaggaaatc gagcgtcgat tgagcgacct ggcgttcgtg ttttaatatc atcccgccgg
     5701 cgtgcggcgg gcacactgca ggtgcgtccc gggacccgtt cgcattgggg aacgtcaatt
     5761 gcggcgactc gtcggtggcg atcgccgcga tgcggggaac cggtttcctc atgacgatcc
     5821 ccggaggggg agtggaacga gtgatccggc cgagtccgga tcgtgagacg cacgtgagga
     5881 ccgagcggta tcggcatgtc gaaaaagaaa tggtggatca tcccggccgt tgcgttcatt
     5941 cccggcatca ttgtcggcgc gctcgcgcta tacttctaca agggcctggt ccgctacaac
     6001 ggcgtcatct ccgcacacgc tggtgagccg gtcaccatcg aacgcgatcg caacggcgtc
     6061 cccgccatcc gcgcgaaggg cgcgacgggt gtgttcttcg ggatcgggtt cgtgcacgcg
     6121 caggaccggc tcgcgcaaat tgaatacttc cgcgccatcg cccgcggccg gctcgcggag
     6181 ctcatcggcg aacacgccgt ggtgcccgac aagctcgcgc gcacggtcgg gtttcaccga
     6241 agcgccgtcg agctcgtgaa gcgcgtgaag agccccgcgc gcgagtacct cgcggcctac
     6301 gccgcaggga tcaacctgta ccgcagcgag atggcccatg agttcatggg gctcctcggc
     6361 gccctgccgg agccctggac cgatgccgat gtcgcgagca ttctcctcct gttcgaatgg
     6421 acggacgcgt tttccgccaa ccgcgaactc atcatgcccg tcgccgacaa tgagcaccgc
     6481 gaggcgctca tgcagatcgt gccccccgtc cttcagtatg cctacggcga cgaggagcga
     6541 agcaccgtgc gcgcgctcaa gggcgtccgt cgcgccctcg cggtgctggt ggatgagtcg
     6601 ccggacgggt ttgcgtttta catccccggc gcccagtcga aggacggacg ggggtatctg
     6661 ggggtgagca ttgccgggag cattgacctg ttcccccggc actacccgtt ggtgatggag
     6721 tcggagcggg gatcgatctc ggggatcacc gcagccggtc ttcccttcat gacgatgggg
     6781 gcgggccccg cgctgtcgtt tgccgggtat acgctccggt gcgatacgca acagttctat
     6841 cttgagaaga tcgctgtggt gtccggcaag gcgatgtatc tgtcgaaggg ggcgttgcgc
     6901 gacttcgagg cgtcgacgga aaccatcgag ataaaaaatc ccggcggccg gcgtgaccgg
     6961 atcgtgttca cggcgcggcg tacggagcgc gggccggtga tcagcgacat ctttagcgac
     7021 cgaatgaaga ccgaggtggt ttccgtcgag gcgcttgcgc ccgatgaggg gctcgtcgct
     7081 gcgctcttcg agctcccctt cgccgactcg gtccaaaaag cgcgcgccgt cattgccgga
     7141 ttgaacgcca acccgcgggc gtttcttttc gccggctcgg acggcgttca agaggcgctt
     7201 gccggccggc tgccggtgat tcagcccccg atgcccgggg ccgtgctccg cgacggttcg
     7261 gcctatgtcg tgcggacggt cgcggacctg tccgggtatg tgcggccgat taccacggag
     7321 ttcgccgtgc tgggccccga gatcctcgac gcggccccgc ctgtcgtgaa ggaggcgatg
     7381 atgccgcgta atacgcatgt gccggcgcgg atcgcggagc atctcgaccg ggacatgctg
     7441 ctggacgagc gggcgtgcga ccgcctgttc gccgacacca cctcgacgct cgccgag
//
LOCUS       DAORHF010000184         7454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9590_length_7454_cov_4.722665, whole genome shotgun sequence.
ACCESSION   DAORHF010000184 DAORHF010000000
VERSION     DAORHF010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7454
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9590_length_7454_cov_4.722665"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="PKJ16_13675"
     CDS             complement(<1..906)
                     /locus_tag="PKJ16_13675"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNV48088.1"
                     /translation="MIYIIYSISIILSIAVFLFLVVHSWRHRATQGARAFFAVNLLGA
                     WGAMAEFFSLLAGSDHLAALWFNFRFVTLGFLPIVWLVFTYQYSGKRGLTARRIALLM
                     ILPAITQVMVWTSDMHGLWLVRDVGFTVVEGFRIADPSQRVPGIWFIVHSVYGFGSLF
                     VGMYLILRTSIRMMRPFRIQGLLIFSGTLILAVGALVPTAKLIPSLTVNPITQSLALC
                     SIFYFLAIFRYRFLNIVPVARDTVIDSMDDAMLVLSMQNHIVDINPAMRTLLAEVASS
                     RGADLDGAFIGERVEDVMAPFGDLVQ"
     gene            complement(1023..2006)
                     /locus_tag="PKJ16_13680"
     CDS             complement(1023..2006)
                     /locus_tag="PKJ16_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HNV48089.1"
                     /translation="MDIIFKDYYQILGIAKGATLDEIKGAFRKKARETHPDATGDAAN
                     YERFVLVREAFDVLTRPAHRAAYDELYEEYYREAASAPHGDYLKSVIDDFDVATSGSY
                     RDEWEFFVRHPDDYLGLFEGTVKLVLGMLLSVAGGIVAPVAAFFGVIALSGSLALLAA
                     VSIAAVAALSLSSLAGAVVALLALRRVMPHAKRLKDSFVALLGRFVVYPLRGIPKRRG
                     SWVLYLNYLIATIAMAAFGYFTASAYWRAIIADAAPGGVSAPVLVFVGAVCGAVIVFT
                     ASVVLIFEIIQEAFKSYPDIRYSRFRVPKGAQIGYASSQGLVGGSDRPRIR"
     gene            2181..2648
                     /locus_tag="PKJ16_13685"
     CDS             2181..2648
                     /locus_tag="PKJ16_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48090.1"
                     /translation="MDIGRIGGPKPIEGIGALSKTTRTTGTAEIAKTDSFQGSDRARA
                     LGRASNIISGEGYTVKSALLERIGDAMYDFGKFTGASKDAIESIADNIARTYLRARES
                     DYSAMDSRYRFNESDTRLNAGARRELVDAIKSGYDYDERLAASANTLLRGYNG"
     gene            2665..3486
                     /locus_tag="PKJ16_13690"
     CDS             2665..3486
                     /locus_tag="PKJ16_13690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV48091.1"
                     /translation="MATPRARRRKTVGVAFGSGGIRGIAHIGVLKSLHEHGISIDCIA
                     GSSVGAWIGAHYALYRDIALLEELTVGNKREKLNSLFEISLRGGLVKGERVKRLVERW
                     LGDSDFESAHVPIRIVACDLVTGEQTVFSSGKLATAVRASLSVPSIFAPFMHDGRALV
                     DGGIVNPVPADVARGMGADVVIAVNLDNFVRGDVFTPHDSASIPRVTNRSVDLLRHYL
                     AQRCSAHADVVIEPLNTRDELSNWKNYFLNDIGMEHIRLGMAAAEEKMPEILRLL"
     gene            complement(3511..5451)
                     /locus_tag="PKJ16_13695"
     CDS             complement(3511..5451)
                     /locus_tag="PKJ16_13695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48092.1"
                     /translation="MKRILIVDDIEENLYLLRALLEGHGYAVDQAGNGSEAIVQALST
                     LPDMVISDILMPVIDGYTLCRIWKADPRLKRLPFVFYTATYTDPRDERLALDMGADAF
                     IVKPAEVEDFLARIETVLDLGGEGKLGAPQSPRIGEEEMLKHYSEALIRKLEHKMFKL
                     EEANRELEATLQAKTQAESALRESEEKFRQLFNLESDAIFLIHNETGEILEANESAAA
                     LYGYAHDELVRMKNTDLSSEPDKTRRAMADHSTVIPIRYHRKKDGSVFPVEITASHLQ
                     WNGQPAHIAAIRDISARMRTEETMRENERIFRLITDNMTDTLWMMDLNFSYSYASPSL
                     LKNRGYTFDELRQLPLERQLTPESYALAASVIAEEMTPERLARKDLQLSRTMELENVR
                     KDGSTFWAEVTMTILRDPDGVPVSILGVGRNITDRKRAEDAIKRSLREKELLLREIHH
                     RVKNNMQVISSLVSLQSNSSQQGDPRAMLTDVQNRIRSIALIHELLYMSDDVSRIDFG
                     VFVGKIVQNLTTAYGASGVTFSITGDAVKLGIDRAVPCGLIMNELISNALKHAFAGKG
                     RGAIAVDLRERGGECVMTVADDGDGLPENVDMRGAKTLGFRLVHLLAEQIGGTIAITR
                     KGGTAFRISFRNEVDAADVSSP"
     gene            complement(5448..5837)
                     /locus_tag="PKJ16_13700"
     CDS             complement(5448..5837)
                     /locus_tag="PKJ16_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48093.1"
                     /translation="MKRTILLIEDNEQNRYLTTYLLRNKGYDVVSADDGHAGIARAEE
                     GGVDLILLDIQLPVMDGYAVAARLREGPATSRIPIVAVTSYAMAGDREKALASGCNGY
                     IEKPINPETFVSEVERYFLNSGTAERT"
     gene            complement(5834..>7454)
                     /locus_tag="PKJ16_13705"
     CDS             complement(5834..>7454)
                     /locus_tag="PKJ16_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV48094.1"
                     /translation="RTVVVGVYENAPKIFTGDDGRPAGIFIDMIQHIAKKEGWTLKYV
                     PGTWAEGLERLARGEIDLMPDVAYTPERAMRFSFHSVPALSSWYQIYAPRGSAINSIL
                     DLNNKRILVLDGSVQQQALARLSASFGLQCTFIAVADYAAMFRAAARGDADGAVTNRF
                     YGLRHAKMYGLEATAVVFEPSDLYFAGRKYVSSALHAAIDTHLAAMRTDHQSVYYRSL
                     KRWSSEEMRYVIPEWLKMAGLLAGIVLVMSIAGSVVLKRQVNARTRELTLVNQEMERR
                     IIERTAELAEAKERAEEADRIKSAFLATMSHELRTPLNSIIGFTGIILQGLAGPLNEE
                     QSKQMGMVRSSARHLLSLINDVLDISKIEAGQLTLNAEPYDVRAVVEKAVAAMRPLAE
                     KKGLALSLFVDPAVGEAHGDQRRVEQVLLNLISNAVKYSERGAVTVSVSEQHGYVPHG
                     EGATRSTEALRLSVADTGIGIRPDDMATLFQPFRQIDSGLTRKTDGTGLGLAICRRLA
                     GLMGGEIVAESEFGRGSVFTFIVPRDVKERS"
BASE COUNT         1463 a         2381 c         2243 g         1367 t
ORIGIN      
        1 ctgcacgagg tcaccgaacg gcgccatgac atcctcgacc cgctcgccga tgaaagcgcc
       61 gtccagatcc gccccccgag aggaggcgac ctctgccagc agcgtgcgca tggcgggatt
      121 gatgtccacg atgtggtttt gcatgctcag gacgagcatg gcatcgtcca tgctgtcgat
      181 gaccgtgtcg cgcgccaccg gtacgatatt gagaaatcga taccggaaga tggccaggaa
      241 atagaagatg gagcacagcg ccaggctctg cgttatggga ttgaccgtga gggacggtat
      301 gagctttgcc gtcgggacga gcgcgcccac ggccaggatc agggttccgg agaatatcag
      361 caatccctgg attcgaaacg ggcgcatcat ccgtatcgaa gtgcggagga tgagatacat
      421 gccgacgaag agcgagccaa acccgtacac ggagtgaacg ataaaccaga tccccggcac
      481 acgctgcgaa ggatccgcga tacggaatcc ctccaccacg gtgaaaccga cgtccctgac
      541 cagccacagg ccgtgcatgt cgctcgtcca caccatgacc tgggtgatcg ctgggagaat
      601 catcaatagc gcgatgcgtc gcgccgtgag gccgcgcttc ccagaatact ggtaggtgaa
      661 tacgagccac acaatcggaa gaaatcccaa ggtcacaaac cggaaattga accacagggc
      721 cgccagatga tcggaaccag caagaagcga gaagaactcc gccatggcgc cccaggcgcc
      781 cagcaggttt acggcgaaaa acgcccgcgc accctgggtt gcacgatgac gccacgaatg
      841 caccaccagg aacagaaata cggcaataga aagtataatg ctgatgctat atatgatgta
      901 tatcatctgc ggcctttgcg tgagcgcccg gccaaacagg tatatctcgg acgagatgtc
      961 ccgtcaagaa aagaaccggc atcccgggag cggcgcgatg gaaaagctct gcggtgtacg
     1021 gatcagcgta tgcggggacg gtcgctccca cccacaaggc cctgtgatga agcgtaaccg
     1081 atctgcgccc ctttcggaac gcggaagcgg ctgtagcgaa tgtccggata gctcttgaag
     1141 gcctcctgga tgatctcgaa gatgaggacg accgacgccg tgaacacgat caccgccccg
     1201 cacaccgccc ccacaaagac cagcaccggc gccgatacgc cgcccggcgc cgcatccgcg
     1261 atgatcgcgc gccagtacgc gctcgccgtg aaatacccga aggcggccat cgcaatcgtc
     1321 gcgatgagat aattcaaata aagcacccac gacccgcgcc gcttggggat tccccgcagg
     1381 gggtacacca cgaatcttcc gaggagggcc acgaaggagt ccttgaggcg tttcgcatgc
     1441 ggcatcaccc gccgcagcgc gagcagggcg acgacggccc ccgccaggga cgagagcgac
     1501 agcgcggcga cggcggcgat cgaaacggcc gcgagcagcg caagcgatcc ggacagcgcg
     1561 atcaccccga aaaacgcagc gaccggcgcg acgatcccgc ccgcgaccga aagcagcatc
     1621 cccaggacca acttcacggt gccttcgaac aggcccaaat agtcgtcggg atggcggacg
     1681 aaaaactccc actcgtctcg gtagctcccg ctggtggcca cgtcgaagtc gtcaatcacg
     1741 ctcttcaggt agtcgccgtg gggggccgat gccgcttcgc ggtagtactc ctcgtacaat
     1801 tcgtcgtagg cggcgcggtg cgcaggccgg gtgagaacat cgaaggcctc gcgcaccagc
     1861 acgaaacgct cgtagttcgc ggcgtcgccg gtcgcatcgg gatgcgtctc gcgcgccttt
     1921 ttccggaagg cgcccttgat ctcgtcgagc gtcgcgccct ttgcaatccc gagaatctgg
     1981 taatagtcct tgaagataat atccattggc ctggccgcat ccggtgcgga ggtaccgccg
     2041 atccgtcggc acatgagata atgatcggcc catttgggca atccatttcc tcgggggatg
     2101 atgatgcgcg aacggaaaaa aactcttgca attccataat tatgtcgcat acttgcacca
     2161 agattcgccc ggaggaaacc atggacatcg gaagaattgg cggaccgaaa ccgatcgagg
     2221 gaattggcgc gctttcgaaa acgacccgga cgaccgggac cgcggagatc gcgaaaaccg
     2281 actcgttcca gggctccgac cgcgcccgtg cgctgggccg cgcctcgaac atcatttccg
     2341 gcgagggcta tacggtgaag agcgccctcc tcgagcgcat cggcgacgcc atgtacgatt
     2401 tcgggaagtt cacgggcgcg tccaaagacg ccatcgagtc catcgccgac aatatcgccc
     2461 gcacctatct cagggcccgc gaatcggact actccgccat ggacagccgc tatcgcttca
     2521 atgagagcga cacccggctg aacgcgggcg cccgccgcga gctggtggac gccatcaagt
     2581 ccgggtacga ttacgacgag cgcctcgccg cctcggcgaa cacgctcctg cgcggataca
     2641 acggctagcg ccgcctgatc cgccatggcc acaccgcgcg cccgtcgccg caaaacggtc
     2701 ggcgtcgcct tcgggagcgg cggcattcgc ggcatcgccc atatcggcgt cctgaaatcg
     2761 ctccacgagc acggcatatc cattgactgc atcgccggct ccagcgtcgg cgcgtggatc
     2821 ggcgcgcact acgcgctcta ccgcgacatt gcgctgctcg aggagctcac cgtgggcaac
     2881 aagcgcgaga agctcaattc gctcttcgag atttcgttgc ggggcggcct ggtcaagggg
     2941 gagcgcgtta agcgcctggt ggagcgctgg ctgggcgaca gcgacttcga atcggcccac
     3001 gtgcccatac gcatcgtcgc ctgcgatctg gtcaccggag agcagaccgt gttctcgagc
     3061 ggaaagctgg cgaccgccgt acgcgcgagc ctatcggtcc cgtccatctt cgcgccgttt
     3121 atgcacgacg gccgcgcgct ggtggacggc ggcatcgtaa atccggtccc ggccgacgtg
     3181 gcgcgcggca tgggcgcgga cgtcgtgatc gccgtcaacc tcgacaactt cgtgcgcggc
     3241 gacgtcttca ccccgcacga ttccgcgtcg atcccgcgcg tgaccaaccg cagcgtggac
     3301 ctgctgcgcc actacctcgc ccagcgctgc tcggcgcacg ccgacgtcgt catcgagccc
     3361 ctcaacacgc gcgacgagct ctccaactgg aagaactact tcctcaacga catcggcatg
     3421 gagcacatac ggctggggat ggcggccgcc gaggagaaga tgccggagat cctgcgactt
     3481 ctgtgatcat cggaccggcg gttcgccatc tcatggcgac gatacatcgg ccgcatcgac
     3541 ctcattgcga aacgagatcc gaaatgcggt cccgcccttc cgcgtaatgg cgattgtccc
     3601 gccgatctgc tccgcaagca gatgcaccag ccggaacccc agcgttttcg cgccgcgcat
     3661 atcgacgttt tcgggaaggc cgtcgccgtc atccgcgacc gtcatcacgc attcgccgcc
     3721 acgctcgcgc aggtccacgg cgatcgcccc gcgcccttta cctgcgaaag catgcttcaa
     3781 tgcgtttgaa atgagctcgt tcattataag gccgcaggga acggcgcggt cgatcccgag
     3841 tttcaccgca tctcccgtga tcgagaatgt gacaccgctc gcgccatagg cggtcgtgag
     3901 attttggact attttaccta cgaatacgcc gaaatctatg cgcgagacgt catcggacat
     3961 gtagagaagc tcgtggataa gggcgattga tcgaatccgg ttctggacgt ctgtcagcat
     4021 ggcgcgagga tcaccctgct gagaggagtt cgactgaagg ctgaccaggc tggagatcac
     4081 ctgcatgttg ttcttcacgc gatgatggat ctccctgagc aggagctcct tctcgcgaag
     4141 cgagcgtttg atcgcatcct ccgcgcgctt gcggtcggtg atattcctcc ccacgcctag
     4201 aatgctcacg gggacgccgt cgggatctcg aagtatggtc atggtgacct cggcccagaa
     4261 ggtggacccg tcctttcgaa cgttctcgag ctccatggtc cgggacagct gcaggtcctt
     4321 ccgcgcaagg cgctcgggcg tcatctcctc cgcaataacc gacgcggcca gggcgtacga
     4381 ttcgggcgtg agttggcgct ccagcggcag ttgacggagc tcgtcgaagg tatagccgcg
     4441 gtttttcagc agggacggac tcgcgtagga atacgaaaaa ttgaggtcca tcatccacaa
     4501 cgtgtcggtc atgttgtcgg tgatgaggcg gaagatgcgc tcgttctccc gcatcgtctc
     4561 ctcggtgcgc atgcgcgccg agatgtcgcg aatggcggcg atatgggcgg gctgcccgtt
     4621 ccactgcaag tgcgaggcgg tgatttcgac gggaaacacg cttccgtcct ttttccggtg
     4681 ataccgaatc ggaatcacgg tggaatgatc cgccatcgcc cgtctcgttt tgtccggctc
     4741 ggacgagagg tcggtgtttt tcattcggac cagctcgtca tgcgcatacc cgtagagtgc
     4801 ggccgccgac tcgttcgcct cgagtatctc gccggtctcg ttgtgtatca gaaaaatggc
     4861 gtccgattcc agattgaaga gctggcggaa cttctcctcg ctctcgcgca gggcggattc
     4921 ggcctgggtt ttcgcctgca aggtcgcctc gagctcccgg ttggcctcct cgagtttgaa
     4981 catcttgtgc tcgagctttc ggatcagcgc ctcgctgtaa tgcttgagca tctcctcctc
     5041 gccgatcctc ggcgattgcg gcgcgcccag cttcccttcg ccgcccaaat cgagcaccgt
     5101 ctcgatgcgg gcgagaaagt cctccacctc cgccggcttc acgatgaacg cgtcggcgcc
     5161 catgtcgagc gcgagccgct cgtcgcgcgg atcggtatag gtggccgtgt agaagacaaa
     5221 ggggaggcgc ttgagccgcg gatcggcctt ccagatgcgg cagagcgtgt atccgtcaat
     5281 gaccggcatg aggatgtccg atatgaccat gtcgggaagc gtcgataacg cctgtacgat
     5341 cgcctcgctg ccgttgccgg cctggtccac cgcatagccg tgcccctcga gcagggcccg
     5401 aaggaggtac aggttttcct cgatatcgtc gacgatcagt atccgtttca tgtccgctcc
     5461 gcagtcccgg aatttaaaaa ataccgctca acctccgaca cgaaggtctc gggattgatc
     5521 ggcttctcga tatacccatt gcatcccgac gcgagggcct tctcgcgatc gcccgccatc
     5581 gcatatgagg tcaccgcgac gatggggatg cggctcgtcg ccgggccctc gcgcaatcgc
     5641 gccgccaccg catacccgtc cataacggga agctggatgt ccagtagaat gaggtcgacg
     5701 cctccctctt cggcccgcgc gatccccgcg tgaccgtcat cggcagaaac cacatcgtat
     5761 cctttgttgc ggagaagata ggtggtcaaa tatcgattct gctcattgtc ttcgataaga
     5821 agtatggttc gtttcatgac cgctccttga cgtcacgggg aacgatgaag gtgaacacgc
     5881 tgcccctgcc gaattcgctc tcggcgacga tctcgccgcc catgaggccg gccagccgcc
     5941 ggcagatggc gagtcccagg ccggtgccat cggtttttcg cgtgaggccg gaatcgattt
     6001 gccggaatgg ctggaacagc gtcgccatgt catcggggcg gatcccgatg ccggtgtcgg
     6061 cgacgctcaa gcgcagcgcc tccgtcgatc gcgtcgcgcc ctccccgtgc ggaacatatc
     6121 cgtgctgctc cgaaacgctc acggtcaccg cgccccgctc ggaatacttc acggcattgc
     6181 taatcaggtt caacaggacc tgttccacgc gccgctggtc gccgtgggcc tcgcccacgg
     6241 cgggatcgac gaacagcgaa agcgccaatc cctttttctc ggccagcggc cgcatcgccg
     6301 cgacggcctt ctcgacgacg gcccgcacgt cgtagggttc cgcattcagc gtaagctgcc
     6361 cggcctcgat cttcgagatg tcgagcacgt cgttgatgag cgacaggagg tggcgcgcgc
     6421 tggagcggac catccccatt tgcttgcttt gctcctcgtt cagggggccc gcaaggccct
     6481 ggagtatgat tccggtgaat ccgatgatcg agttgagggg cgtgcgcagt tcatgggaca
     6541 tcgtcgcgag gaaggcggac ttgatccgat cggcctcctc ggcgcgctcc ttggcctcgg
     6601 ccagctcggc ggtgcgctcg atgatgcgcc gctccatctc ctggttgacc agcgtgagct
     6661 cccgcgtgcg cgcgttcacc tgccgcttga gcaccacgct cccggcgatg ctcatgacca
     6721 gcactatgcc cgccagaaga cccgccatct tgagccactc cggtattaca taccgcatct
     6781 cctcggacga ccaccgcttg agggagcggt aatagaccga ttgatggtcc gttctcatgg
     6841 ccgcaaggtg ggtgtcgatg gcggcgtgca gtgcgcttga aacgtatttt cgtccggcga
     6901 aatacagatc ggacggctcg aacacgacgg ccgtcgcctc gagcccgtac atcttcgcat
     6961 ggcgcaaccc gtaaaaacgg ttggtcacgg ccccgtccgc atcacccctg gccgcggccc
     7021 tgaacatggc ggcataatcg gcaacggcga taaacgtgca ctgcaacccg aaacttgcgc
     7081 tgagtcgtgc aagcgcctgc tgttgcacgg agccgtccag aacgagtatc cgcttgttgt
     7141 tcaaatcgag gatcgagttg atggcgctcc ccctcggtgc atagatctga taccatgatg
     7201 acagcgccgg taccgaatgg aaggaaaacc gcatcgcccg ctccggcgta taggcgacat
     7261 cgggcatgag gtcgatctcc ccgcgcgcca gccgttccag tccctcggcc caggtgccgg
     7321 ggacatattt cagggtccat ccttcttttt tcgcgatgtg ctggatcatg tcgatgaaga
     7381 tgcccgccgg ccggccgtcg tcgccggtga atatcttggg cgcgttttca tacacgccga
     7441 cgacgaccgt ccgc
//
LOCUS       DAORHF010000185         7428 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9636_length_7428_cov_5.875899, whole genome shotgun sequence.
ACCESSION   DAORHF010000185 DAORHF010000000
VERSION     DAORHF010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9636_length_7428_cov_5.875899"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKJ16_13710"
     CDS             complement(<1..469)
                     /locus_tag="PKJ16_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HNV48095.1"
                     /translation="MKIADIGGEFAFIRRVAREYRDPAIVRGAGDDCAAIDHPPSRLL
                     LLTTDMMVENTHFTRDWFTPRQIGRKLMEVNVSDIVSMGGVPRHAVISLAMPPDTTIE
                     FMDELYRGLYESAEAHGVALVGGDTTRGRELTLNLALTGEVEPELVRYRSGALP"
     gene            complement(466..1737)
                     /gene="thiC"
                     /locus_tag="PKJ16_13715"
     CDS             complement(466..1737)
                     /gene="thiC"
                     /locus_tag="PKJ16_13715"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HNV48096.1"
                     /translation="MTQLEHARENRITPEMESVAQREPRAAEEICRDIAAGRTVICKS
                     DLHDCVPVAIGAGLAIKINANIGTSGYNADPALELEKLAASVRYGADTVMDLSTGGDI
                     RGMRRLIVKESRVPVGTVPIYEAIVKEPDVAAITEDDFLASIRMHIDDGVDFITVHCG
                     ITRACVPLLESRIMGVVSRGGSFLIKWMLHHEKENPLYTRYDEILAMAKEHDVVLSLG
                     DGLRPGCLADATDSAQIEELKTLGELAARAKAAGVQVMIEGPGHVPLHMIKENIELQK
                     KYCDGAPFYVLGPLVTDISPGYDHLTGAIGGALAAYYGADYLCYLTPTEHLGLPGVQD
                     VIDGVIASKIAAHAADIARGIPGSRDRDDAMSRARANLDWEGMYRQSLNPDRARELRA
                     RAGADDLEVCTMCGEFCSARINRECRTRYGK"
     gene            complement(1734..2345)
                     /gene="thiE"
                     /locus_tag="PKJ16_13720"
     CDS             complement(1734..2345)
                     /gene="thiE"
                     /locus_tag="PKJ16_13720"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HNV48097.1"
                     /translation="MKGYYFITDSGLSAGGTMHDVRCAVMAGVRVVQYREKDAPSGVM
                     YREAHEIAELCAGSATRLIINDRVDIALAVGADGVHIGQDDLPYPVARRLLGPDRIIG
                     VTVHSLDEAIDAERAGADYLGVSPIFATGTKLDAGHPCGVETLAKIRAACRLPLVAIG
                     GIDLENVDEVISAGADMACAISAVVAKSDVPEEILQFQRKFGL"
     gene            complement(2342..3145)
                     /gene="thiM"
                     /locus_tag="PKJ16_13725"
     CDS             complement(2342..3145)
                     /gene="thiM"
                     /locus_tag="PKJ16_13725"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015413156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HNV48098.1"
                     /translation="MMDVYAYLKAVHDRQPLVHHITNWVTIYDCAQAVKSLGASPVMA
                     HAPEEAADMASLAAAVALNIGTLTDEVVEAMKLAAQAANRKGAPVVLDVCGAGATPYR
                     DRKNIELLDETRIDVIKGNASEIARIAGRDVHTKGVDAGHVADDLQKIAMELAAERAC
                     VVAVTGPIDIVADAQRAISIANGHEMMTRVVGTGCMATSLIATFVGASPGNLLEAAAA
                     ALACYGIAGEIAAGKADGPASYKTLLLDELYRLSQLDAKSIATRARVSG"
     regulatory      complement(3197..3294)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3473..4141
                     /gene="mobB"
                     /locus_tag="PKJ16_13730"
     CDS             3473..4141
                     /gene="mobB"
                     /locus_tag="PKJ16_13730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HNV48099.1"
                     /translation="MNIPIVSIVGRSNTGKTTLVEKLVAELTRRGRRVATIKHNLHGF
                     DIDHEGKDSFRHKQAGAVMTVIASAARVALVKDIEGDMTIDEIAARFIDDADIIITEG
                     YKGNDKAKIEVHRREAYPDLLCGAGDNLLAVATDTRLSIDVPQYDINDAPGLCDLIER
                     EALAATRGPAVTLTVDGAVVPLKPFVQAMLANGIRGMVSGLKECGDARELRIAISARA
                     PEER"
     gene            4158..4739
                     /locus_tag="PKJ16_13735"
     CDS             4158..4739
                     /locus_tag="PKJ16_13735"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HNV48100.1"
                     /translation="MSISGVILSGGKNSRFGSNKAFVEFDGARIIDIALAAHARRFAE
                     VLIVTNEPDAYQGLDARIVTDIHPNFGSLGGIYTGLWHASGEAVFVTACDMPFVDPAV
                     MDLVLSRFEDVDIVVPRIDGYYEPLMAIYAKACLPHMAASIEANIRQIIRFFPQVRVR
                     EIPEADIRVVDPALRTFVNINTREDLDRALSPP"
     gene            4747..5994
                     /locus_tag="PKJ16_13740"
     CDS             4747..5994
                     /locus_tag="PKJ16_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNV48101.1"
                     /translation="MQTIALVIFIITYTGIIFTRLPWVNVDRPSAAFFGAVAMVLCGV
                     ITFDEAVRSIDANTIALLLGMMIVVATLELDGFFSLIARRTIALAKTRLRLLAAVTFV
                     TGIASAFLVNDAVVLLFTPVVIAICRASRVDPIPYLIAEILASNVGSAMTITGNPQNM
                     LIGINSGIPYARFLLCLLPISLLGLCIVVAVVRAVYRRHFAELGPIEYDENGFEYNYA
                     SMKFSVPIFALVIVLFFLSHLLHLSIPVIALVGASLVLIFGKIKPSTVIKSIDWVLLL
                     FFASLFIVVHGVEAAGLMSHVHDVGTLRPDIAGLGIIHALSLALSQIVSNVPFTIVML
                     PLMKNAGSEALWLALASASTLAGNATIIGAMANLIVIESAQGLGVRIGFREFLRPGVI
                     VTLATLALSMGVLALEVAFGVIR"
     gene            6137..7183
                     /locus_tag="PKJ16_13745"
     CDS             6137..7183
                     /locus_tag="PKJ16_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48102.1"
                     /translation="MRRLADRLLGRGMGQLGERFLWPVLLSDLARYLDAMKFTRVPRL
                     TADGLESFRAAAGRRDDVRGAIEDFISGGSDLYFESVGTVKMLAETDAGSFHHLLEIA
                     VRRRHARDVAPIEIVVNSIDEDPATGVAQEVESFRQFVDRLELTARKHLPVGEIAVEY
                     TGRITVDGGGSSGAENGAIIFDISGMANSGAGPPALFPLYGVTLGRTTERELAGMGRH
                     ATGKDDRGQVFKYYVISGMNFWYENGVVNSMYITNSDPLPTQWRALGFSWELSYAKWI
                     ALCAQRGFEVQEVTPPGTIPHFSGSRMTFSAEIVVIWREESGEYPIRFVFNYGEGTTA
                     KDSDTLYSIGIRLE"
     gene            complement(7233..>7428)
                     /locus_tag="PKJ16_13750"
     CDS             complement(7233..>7428)
                     /locus_tag="PKJ16_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48103.1"
                     /translation="YLYLFFHRENAYPFLIFGMALKYWAFLTSLIAYRRYRIPAAVFF
                     NFGLGNLAVGILFSAYLLMR"
BASE COUNT         1229 a         2396 c         2429 g         1374 t
ORIGIN      
        1 ccgggagggc gcccgagcga tagcggacga gctcgggctc gacctcgccc gtgagcgcga
       61 gattgagcgt gagctcgcgt ccccgcgtgg tgtcgccgcc cacgagcgcc acgccgtgcg
      121 cctccgccga ttcgtatagt ccccgataga gctcgtccat gaactcgatc gtggtgtcgg
      181 gcggcatggc aagcgagatg acagcgtggc gcggcacgcc tcccatcgac acgatgtccg
      241 agacgttcac ctccatgagc ttcctgccga tctggcgcgg cgtgaaccag tcgcgcgtga
      301 agtgcgtgtt ctcgaccatc atgtcggtgg tcaggaggag caggcggctc ggtgggtggt
      361 cgatcgccgc gcagtcgtcg cccgcgccgc gcacgatcgc cggatcacga tactcacgcg
      421 cgacgcgccg gatgaaggcg aactccccgc cgatgtccgc gatcttcatt tcccgtaccg
      481 ggtccggcac tcgcggttga tgcgcgccga gcagaactct ccgcacatgg tgcacacctc
      541 gaggtcgtcg gctccggcgc gggcgcgaag ctcgcgcgcc cggtcgggat tgagcgactg
      601 ccggtacatg ccctcccaat cgaggttggc gcgggcgcgg gacatcgcgt cgtcgcggtc
      661 gcgcgagccg ggaatgccgc gggcgatatc ggccgcgtgc gcggcgatct tcgaggcgat
      721 taccccgtcg atcacgtcct ggacgcccgg gaggcccaaa tgctccgtgg gcgtgagata
      781 acagaggtag tccgcgccgt agtacgccgc gagcgccccg ccgatggcgc cggtgagatg
      841 gtcgtagccc ggcgagatgt cggtgaccag cggcccgagc acatagaagg gcgcgccgtc
      901 gcagtatttt ttctggagct cgatgttttc cttgatcatg tggagcggca catggccggg
      961 gccctcgatc atcacctgga cgccggcggc ctttgcgcgc gcggcgagtt cgcccagcgt
     1021 cttgagttcc tcgatctgcg cgctgtcggt ggcgtcggcc agacagccgg ggcgaaggcc
     1081 gtcgcccagg ctcaggacca cgtcgtgctc cttcgccatc gcaaggatct cgtcgtagcg
     1141 cgtatagagc gggttttcct tttcgtggtg gagcatccac ttgatgagaa agctcccgcc
     1201 gcgcgagacc acgcccatga tgcgggattc aagcaggggg acgcaggccc gcgtgatccc
     1261 gcagtgcacg gtgatgaaat ccacgccgtc gtcgatgtgc atgcggatgg acgcgagaaa
     1321 atcgtcctcc gtgatcgcgg cgacatcggg ctccttcacg atcgcctcgt agatgggcac
     1381 cgtgcccacc ggcacgcgcg actccttcac gatgagcctg cgcatgccgc ggatgtcgcc
     1441 tccggtggag aggtccatca ccgtgtcggc gccgtagcgg accgaggcgg cgagcttctc
     1501 gagctcgagg gcgggatcgg cgttgtagcc ggaggtcccg atgttcgcgt taattttgat
     1561 ggcaagcccc gcgccgatcg cgaccgggac gcagtcgtgc aggtcgctct tgcagataac
     1621 ggtgcgcccc gcggcgatgt cgcggcaaat ctcctccgcc gcgcgcggct cgcgctgcgc
     1681 gaccgactcc atctcgggcg tgatacggtt ttctctcgcg tgttcgagct gtgtcataag
     1741 ccaaacttcc tctggaactg gagtatctcc tcgggcacat cgctcttggc gaccaccgcg
     1801 gagatggcgc aggccatgtc ggcccccgct gaaatcacct cgtcgacgtt ctcgaggtcg
     1861 atgccgccga tcgcgaccag cggcagccgg caggcggcgc ggatcttcgc cagcgtttcg
     1921 acgccgcagg gatgccccgc gtcgagcttg gtgccggtgg cgaagatggg gctcaccccg
     1981 agatagtcgg cgcccgcgcg ctcggcgtcg atcgcctcgt cgaggctgtg gaccgtgacc
     2041 ccgatgatgc ggtccggtcc cagcaggcgg cgcgcgaccg ggtagggcag gtcgtcctgc
     2101 ccgatgtgca cgccgtcggc gccgaccgcg agcgcgatgt cgacgcgatc gttgatgatg
     2161 agcctcgtcg cgcttcccgc gcacagctcc gcgatctcat gggcctcgcg gtacatgacg
     2221 cccgagggcg cgtccttttc ccggtactgg accacgcgca cgcccgccat cacggcgcag
     2281 cggacatcgt gcatggtgcc gccggcgctc aagccggaat cggtgatgaa gtagtacccc
     2341 ttcatcccga cacccgggcg cgcgtcgcga tcgatttcgc atcgagctgc gaaagccggt
     2401 agagctcgtc gagcaggagc gttttatacg aggccgggcc gtcggccttt ccggcggcga
     2461 tttcccccgc gatcccgtag caggcgagcg cggccgcggc ggcctcgagc aggttgcccg
     2521 gtgaagcacc gacgaaggtc gcgataagcg acgtggccat gcagccggtc cccaccacgc
     2581 gcgtcatcat ctcgtggccg ttcgcgatgc tgatggcgcg ctgcgcgtct gcgacgatgt
     2641 cgatcggtcc cgtgaccgcc acgacgcagg cgcgttccgc cgcgagctcc atcgcgatct
     2701 tctggagatc gtcggccaca tggcccgcat cgacgccctt cgtgtgcacg tcgcgccccg
     2761 cgatccgcgc gatctccgag gcgttcccct tgatgacgtc gatgcgcgtc tcgtccagga
     2821 gttcgatgtt cttccggtcg cggtagggcg ttgcgcccgc gccgcagacg tcgagcacga
     2881 cgggcgcgcc cttcctgttt gcggcctgcg cggcgagctt catggcctcc acgacctcgt
     2941 cggtgagcgt gccgatgttg agcgcgacgg ccgcggcgag cgacgccatg tcggcggcct
     3001 cctcgggggc gtgcgccatc acgggcgagg cgcccagcga cttcacggcc tgcgcgcaat
     3061 cgtagatggt gacccagttg gtgatatggt gcacgagcgg ctgccggtcg tgcaccgcct
     3121 tcaggtacgc ataaacatcc atcatgacaa ctccttcatt tagaatgcga tgatgggtgt
     3181 ggtgttgagt ggagagagag ttatgcgacg cgcgtccctt cgctggcatt acccagatca
     3241 ggttcaacgg gtataatctc aggctccggt gcggaaccac cccatggcgg atcaaacata
     3301 cggacggtgc gcgcttgagt caagcggttt gatgataaaa tggggaaata tgatggtgcg
     3361 gtgatgcgat caggcgatcg ccggccccgt caccggcaaa aatgttcttg cgcccgctcc
     3421 gcgccgacgc tactacggca ccggtcgcgc gggccatgcg cgggaggcgt gtatgaacat
     3481 acccatcgta tcgatcgtcg ggcggtccaa tacgggcaag accacgctgg tggagaagct
     3541 cgtcgcggag ctcacccggc gtggtcgccg cgtggcgacc atcaagcaca atctccacgg
     3601 cttcgacatc gaccatgagg gcaaggacag cttccgtcac aagcaggccg gcgcggtgat
     3661 gacggtcatc gcctcggccg cgcgcgtggc gctggtcaag gacatcgagg gcgacatgac
     3721 gatcgatgaa attgccgcgc gtttcatcga cgacgccgac atcatcatca ctgaaggcta
     3781 caaggggaac gacaaggcca agatcgaggt gcaccggcgc gaggcctatc ccgatctcct
     3841 gtgcggcgcg ggggacaatc tcctcgccgt cgcgaccgat acgcgcctct cgatcgatgt
     3901 gccgcagtac gatatcaacg acgcgcccgg gctgtgcgat ctcatcgagc gtgaggcgct
     3961 cgccgcgacg cgcggccccg cggtcacgct cacggtggac ggggctgtcg tcccgctcaa
     4021 gccgttcgtg caggccatgc tcgcgaacgg aatccgcggg atggtgtccg ggctcaaaga
     4081 atgcggcgat gcgcgggagc ttcgaatcgc gatctcggcg cgcgcgccgg aggaacgatg
     4141 acgatggaac aggcgcgatg agcatctccg gcgttatcct gagcggcgga aagaacagcc
     4201 ggttcggatc gaacaaggcg tttgtcgaat tcgatggcgc gcgcatcatc gacatcgcgc
     4261 tcgcggcgca cgcgcgccgc ttcgccgagg tgctcatcgt caccaacgag cccgatgcgt
     4321 atcagggcct cgatgcgcgc atcgtcaccg acatccatcc gaacttcggg tcgctcggcg
     4381 ggatctacac cgggttatgg cacgcgagcg gcgaggcggt cttcgtcacg gcctgcgaca
     4441 tgccgttcgt cgatccggcc gtcatggacc ttgtgctctc gcggttcgag gatgtcgata
     4501 tcgtcgtgcc gcggatagac ggctactacg agccgctcat ggcgatctat gcgaaggcgt
     4561 gtctgccgca catggctgcg agcatcgagg cgaacatcag gcagataatc cgcttctttc
     4621 cgcaggtgcg cgtgcgcgag atacccgagg cggacatccg ggtcgtggac cccgcgctgc
     4681 ggacgttcgt gaacatcaat acgcgcgagg acctcgatcg cgctctctct ccgccgtaac
     4741 acggccatgc agaccatcgc gcttgtcatc ttcatcatca cctacaccgg tatcatcttc
     4801 acgcggttgc cgtgggtgaa cgtggaccgg ccctccgcgg cttttttcgg ggccgtggcg
     4861 atggtcctct gcggcgttat cacattcgac gaggccgtgc gctccatcga cgccaacacc
     4921 atcgcgctcc ttctgggcat gatgatcgtg gtcgcgaccc tggagctcga cggcttcttc
     4981 tcgctcatcg cccgccgcac gatcgccctc gcgaaaacgc ggttgcggct cctggcggcg
     5041 gtcacgttcg tgacggggat cgcgagcgcc ttcctggtga acgacgcggt ggtgcttctc
     5101 tttacgcccg tcgtcatcgc catctgccgc gcctcgcgcg tggatcccat accctatctc
     5161 atcgccgaga tcctcgcctc gaacgtggga agcgcgatga ccatcaccgg caatccgcag
     5221 aacatgctca tcggcatcaa ctccggcatt ccgtacgcgc gcttcctgct gtgcctgctg
     5281 cccatttcgc tcctgggcct gtgtatcgtc gtggccgtgg tgcgcgcggt ctatcgccgc
     5341 cacttcgccg agctcggccc catcgaatac gacgagaacg gtttcgagta caattacgct
     5401 tcgatgaagt tctccgtgcc catctttgcg ctcgtcatcg tcctcttttt tctcagccac
     5461 ctgctccatc tctccattcc ggtgatcgcg ctggtgggcg catcgctcgt gctcatcttc
     5521 ggaaagatca agccgtccac cgtcatcaag agcatcgact gggtgctcct cctcttcttc
     5581 gcgtcgctgt tcatcgtcgt gcacggcgtc gaggcggcgg ggctgatgtc gcatgtgcac
     5641 gacgttggaa cgctccgccc cgacatcgcg ggcctcggca tcatccacgc cctgagcctc
     5701 gcgctctcgc agatcgtgag caatgtcccg ttcacgatcg tcatgctgcc gctgatgaaa
     5761 aacgccggca gcgaggcgct ctggcttgcg ctcgcctcgg cctcgaccct cgcgggcaac
     5821 gcgaccatca tcggcgcgat ggccaatctc atcgtgatcg aatcggcgca gggcctgggc
     5881 gtgcgcatcg gcttccgcga gtttctgagg cccggcgtca tcgtcacgct tgcgacgctc
     5941 gccctttcga tgggggtgct cgccctcgag gtcgcgttcg gggtgatccg ctgacgcgcg
     6001 tcgagcgaac cgcgaaatta tcaattgaca cgatgccggg ggcgcgatac gctcagcggt
     6061 catgttgcac aaagcgattg tcaaaatacg cctgagtgat acgccggtcc cgtcgccgcg
     6121 tcgggacggc ggtttgatgc ggcggttggc ggaccggctc ctcggacggg ggatgggaca
     6181 gctcggcgag cgcttcctgt ggcccgtact cctgagcgat ctggcgcgct acctcgatgc
     6241 aatgaagttt accagggtcc ccaggctcac cgccgacggt ctggagtctt tcagggccgc
     6301 tgccggaaga cgcgacgatg tccgcggggc gatcgaggat ttcatctccg gcgggagcga
     6361 tctgtatttt gaaagcgtgg gcaccgtaaa gatgctcgcg gagaccgacg ccggttcctt
     6421 ccatcatctt cttgaaatcg ccgtgcggcg ccgtcacgcg cgcgacgtgg cgcccatcga
     6481 gatcgtcgtc aactctatcg acgaggaccc cgcgaccggc gtcgcgcagg aggttgaatc
     6541 gttccggcag ttcgtggacc gcctcgaact cacggcgcga aagcacctgc cggtgggcga
     6601 aatcgcggtg gagtacacgg gacgcatcac cgtggacggc ggaggaagct cgggggctga
     6661 gaacggcgcg atcatcttcg atatctccgg gatggcgaat tcgggtgcgg ggccgcccgc
     6721 gcttttcccg ctgtatgggg tgacgctggg gcggacgacg gagcgcgagc ttgcgggcat
     6781 gggccggcat gcgaccggaa aggacgaccg cgggcaggtg tttaaatact acgttatttc
     6841 cggcatgaac ttctggtacg agaacggcgt cgtgaactcg atgtatataa ccaattccga
     6901 tccgctgccg acgcaatggc gcgccctcgg gttttcatgg gagctttcgt atgcgaaatg
     6961 gatcgcgctg tgtgcacagc gcggcttcga ggtccaggag gtgaccccgc ccgggaccat
     7021 accgcacttc tccggcagtc gaatgacctt ttcggccgag atcgtcgtca tctggcgcga
     7081 ggaaagcggc gagtacccga tacggtttgt cttcaattac ggcgagggga caacggcgaa
     7141 ggattccgac acgctgtaca gcatcggcat acggctggag tgagctcgat ctcgttctgc
     7201 ggggcggggc gcgaccatcc gccgccgctc cattagcgca tcaacaggta tgccgagaag
     7261 agtattccca ccgcgagatt gcccagccca aagttgaaga aaaccgccgc gggaatccga
     7321 taccgccgat acgcgatcag gcttgtgaga aacgcccagt atttgagcgc catgccgaag
     7381 atcaggaagg ggtacgcatt ctcccgatgg aagaacaaat agaggtag
//
LOCUS       DAORHF010000186         7377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9757_length_7377_cov_5.244196, whole genome shotgun sequence.
ACCESSION   DAORHF010000186 DAORHF010000000
VERSION     DAORHF010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_7377_cov_5.244196"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            182..1033
                     /locus_tag="PKJ16_13755"
     CDS             182..1033
                     /locus_tag="PKJ16_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV48104.1"
                     /translation="MKHYRRWFRPFVVLVAIALWAGAGCQSSFVYYPDRAIVRYPNAY
                     GLAYESITLTTADRVRINAWWVPAGDGAPVALFFHGNAGNNSHRADHLALFHRMGVSV
                     FIVDYRGFGKSEGSPSERGTYEDARAAWAYLTKERGIAPGRIVVFGQSLGGSVAAWLA
                     REAAPGPGALILDSTFTAIADVARQSCLYYPLGWIILTYRYDTRAYVKGARCPVMVIH
                     SREDELIHFSKGQRLYAEAPGPKRFLEIRGSHNTGFITSMREYERGIRDFIETYVGIK
                     KRRTAVE"
     gene            1033..1566
                     /locus_tag="PKJ16_13760"
     CDS             1033..1566
                     /locus_tag="PKJ16_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48105.1"
                     /translation="MMIRVCASIFACVAFLAAAAPIGFAPPTKAIALIAESVDEGCAV
                     CVDDLRKKAFADLAKAFAPGTMVKVQGGCVFVKPKGSTANDLAPACYAPGANEPRIVF
                     RFYTKGRRLAGIDAGDFTDRQIERTYARAPKSVRFDATVRIIPYKYGDGPAFNYFAKE
                     NSIVIHCAVVELKAAGR"
     gene            complement(1675..2916)
                     /locus_tag="PKJ16_13765"
     CDS             complement(1675..2916)
                     /locus_tag="PKJ16_13765"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HNV48106.1"
                     /translation="MIHLTTALAIACGAIGCGHAPVQYDLPSGSKRRVLTGLEVFIGE
                     RAKDYRGRTAALVSNHSGVDHSLRANVELLRASGISIALAFAPEHGLHGHQNAYDTRG
                     YIVDERRNLMVYNLHLLTVDSLRHLLRRVDLVIYDIQDMGMRCYTYVSDLKMVMDALA
                     GTGTELVVLDRPNPLGFLGVDGPPLDRRFISRHIASFPAPFIYGLTIGEAARYYNGEA
                     GTKVRLTVVPMKNYGRGLLYHETMLPWVPPSPNLPTYPSSIVYAGIVLLEGINLSVGR
                     GTTKPFEYIGAPWIDPFRSSEALNALKLPSFRFRPVYFEPTFSKYKNRTCGGVQIFYT
                     GGRFSPTEFTYILLRHLFDTYPECRWERAGGTYDIDVLAGTDRIRKLVDAKRDWRDLA
                     RELAPQLDAYRKTSARYHLYR"
     gene            3094..3720
                     /locus_tag="PKJ16_13770"
     CDS             3094..3720
                     /locus_tag="PKJ16_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48107.1"
                     /translation="MYRALLLLMTLVQVASCQTMHEREKVILDYSRQAGAGRSAYEQY
                     LVQEDQYTTNGVVYHRKYRTYLLGIARYMIEEKKYAVLKNCIGFYFDKRENIKSRLYL
                     GLDVELPVDASLASSSGRGIALSFFNKHLSEVLYVLFSCKTIFAEAEVVGAVVGFQWR
                     AAGSQEIYNIWIDKKDVIKFENGSLTLREIVERNIITDTRGKVVKLSQ"
     gene            complement(3704..4756)
                     /gene="hisC"
                     /locus_tag="PKJ16_13775"
     CDS             complement(3704..4756)
                     /gene="hisC"
                     /locus_tag="PKJ16_13775"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HNV48108.1"
                     /translation="MKYWNARLKKMSEYVPGEQPENLSEFIKLNTNENPFPPPASVTE
                     AMRKACDESIRRYPSPTSLSAREAFAKEHALGVENVMLGNGSDEIFTLLFRGFIEPKG
                     LAAFPYPSYSLYYTLAEANGIKYEKLPLTASLDVNLDEYLKKPYDLVIIGNPNNPTGR
                     GVEVKAIRAFLGKFAGLLVVDEAYVDFYGETAIGLVADFDNIIVTRSLSKSCSLAGMR
                     VGFAAASKEIIQGLLKLKDSYNIDRVAEAAARAAILDRKGVAYNISMLRSNKEYLEER
                     LADMDFEVVPSKANFLFVRHPSVPAADLFEQLKERKILVRYFTGPVQGEYVRITVGTM
                     MEIKTLVKELGAIVTA"
     gene            complement(4769..5743)
                     /locus_tag="PKJ16_13780"
     CDS             complement(4769..5743)
                     /locus_tag="PKJ16_13780"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HNV48109.1"
                     /translation="MDLSVNIGPLTLTNPVTLASGTAGYGEELAKYYDLSRIGAIFTK
                     GLSLKPRAGNAGNRILETPAGVLNSIGLENVGLEKFLSDKLPPLAATGVAVIPNVAGH
                     SVDENVDLCRELSNAPGVAALELNVSCPNVKEGGIAFGRDPKVFADLVEKTRAVTRCA
                     LIVKLSPNVTDITEFAKIAEERGADAVSAVNTYLGMKIDIDARRPHFRNRVAGLSGPA
                     IRPMAVRAVYEIFERTRIPIVGIGGIATAEDALEFIMAGASAVSIGTMNMVHPDAGIR
                     CLQGIEEFCRAERIGTVRDLIGCAHGDRVPASSACDAAQPINTPTTGP"
     gene            5902..6618
                     /locus_tag="PKJ16_13785"
     CDS             5902..6618
                     /locus_tag="PKJ16_13785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV48110.1"
                     /translation="MAVLAFDVDGTIFDSDSIIVDAFAGGIDDFMRRQPGARLDLPAK
                     SDIIAQIGNPAGAIFSALFPGLDADSRSLMERLCTERLSAMVRSGGGGLIDGAAETLA
                     ALARDGHRFLVASNGRIEYLRSILETHGLWRLFERPVLCIDRASGRRDKTDIVREYVR
                     LHSSGNGVFVMIGDRGSDMAAARGNGVAFIGCAFGHAGDAEISSARHIARRFGEIPAL
                     VKIIEASFPTPRIMHCGLLE"
     gene            complement(6636..>7377)
                     /locus_tag="PKJ16_13790"
     CDS             complement(6636..>7377)
                     /locus_tag="PKJ16_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV48111.1"
                     /translation="WNGIPQALRDEGAKVYITSVNGMDSTANKAASFKAQFLNIKAVT
                     GAAKLNVIGHSHGTIYTRYAISNLALSSYVASHTSIAGPHRGSAVADVILGVVPNVAQ
                     PLVGATLDFVYAFIFGDKNPNSLQNGYDLVRSYMINTFNPNTPNKSGIYYQSWAAKIK
                     TMAIDARNWYFLVTWPILLYYEGANDGLVSVTSAQWGTFRGTHEGAWYSAGVDHLNII
                     DQPFGITPGFDAPDFFVGIVSELKGKGY"
BASE COUNT         1313 a         2244 c         2395 g         1425 t
ORIGIN      
        1 ttttaaatca gcagtttcgg ataaacgcga acaatgccgt gtcgctcgag ctcaagcata
       61 cgataacctt cggctacacg gattcggtgg ccggcctgtc gtataatctg ttcttttgac
      121 ggatcgcgca tcggcgcgcg ttcgtaaaac aatacggcct cgatgatttg ggggtgttgc
      181 cgtgaagcat tatcgtcgct ggttccgccc gtttgtagtt cttgtcgcga tcgccctgtg
      241 ggccggcgcc ggctgccagt cctcgttcgt ctactatccc gatcgcgcga tcgtccgcta
      301 tcccaacgcc tacgggctcg cctacgagtc gattacgctc accacggccg accgcgttcg
      361 catcaacgcc tggtgggttc ccgcgggcga tggcgcgccg gtggcgctct tctttcacgg
      421 gaacgcgggc aacaattccc atcgcgccga ccacctcgcg ctcttccacc gcatgggcgt
      481 gagcgtgttc atcgtcgatt atcgcggctt cgggaaaagc gagggctctc ccagcgagcg
      541 cggcacctac gaggacgcgc gcgccgcatg ggcctatctc acgaaggagc gcgggatcgc
      601 gcccggtcgc atcgtcgtct tcgggcaatc gctgggggga tcggtcgccg cgtggctcgc
      661 gcgggaggcg gctcccgggc ccggcgcgct catcctcgac tccacgttca ccgcgatcgc
      721 cgacgttgcg cggcagagct gcctgtatta tccgctcggc tggatcatcc tcacctatcg
      781 gtacgatacg cgcgcgtacg taaagggcgc gcgctgtccc gtcatggtga tccacagccg
      841 cgaggacgag ctcatccact tttcgaaggg acagcgcctc tatgccgagg cgccgggccc
      901 caagcgattc ctggagatcc gcggatcgca caatacggga ttcatcacct cgatgcgcga
      961 atacgaacgc ggcattcggg attttatcga aacatatgtc ggaatcaaaa agagaagaac
     1021 ggcggtggag taatgatgat aagagtatgt gcttccattt tcgcgtgtgt cgcgtttctc
     1081 gcggcggcgg cgccgatcgg gtttgcgccc ccgacgaagg cgatcgcgct catcgccgag
     1141 agcgtcgacg agggctgcgc ggtctgcgta gatgatctga ggaaaaaagc cttcgcggat
     1201 ctcgcgaagg cctttgcgcc ggggacgatg gtgaaggtgc agggcggttg cgtctttgtg
     1261 aagccgaagg gatcgacggc aaacgatctc gcgcccgcct gctacgcgcc aggagcgaac
     1321 gaaccgcgca tcgtctttcg cttctacacg aagggcaggc gtctcgccgg gatcgatgcg
     1381 ggcgatttca ccgaccggca gatcgagcgc acctatgcgc gcgcgcccaa gagcgtgcgt
     1441 ttcgacgcga cggtgcggat catcccctac aaatacggcg acggtcccgc ctttaattac
     1501 tttgcgaagg aaaacagtat tgtcatccac tgtgcggtgg tcgaacttaa ggcggcgggt
     1561 cgctaatagc ctgtttcagg gattcatcac caatagcacc gagggagacg gcatgatgtc
     1621 acgctcgtct cgccgcgaac agtacatcca tgtattcgcg gctgcggcag ccgatcaccg
     1681 atacagatgg tatcgcgcgc tcgtcttccg gtacgcgtcg agctgtgggg cgagctcacg
     1741 ggcgaggtcg cgccagtcgc gtttcgcatc aaccaatttt cgtatgcgat cggtccccgc
     1801 gagaacgtcg atatcgtagg ttccgcccgc gcgctcccag cggcattccg ggtaggtgtc
     1861 gaacaggtgc cgaagaagga tataggtgaa ctccgtggga ctaaagcgcc cgccggtgta
     1921 aaaaatctgc acgcccccgc aggtgcggtt cttgtacttg gagaaggtcg gctcgaaata
     1981 cacgggacgg aagcggaacg acgggagctt gagcgcgttg agcgcctcgc tcgaacggaa
     2041 ggggtctatc cacggcgcgc caatgtactc gaacggcttg gtggtcccgc gccccaccga
     2101 caggttgatc ccctcgagga gcacgatccc ggcatacacg atggacgatg ggtaggtcgg
     2161 gaggttcggc gacggcggca cccacggcag catggtctca tgatagagaa gcccgcgccc
     2221 atagttcttc atgggcacta ccgtgaggcg cacctttgtg ccggcctcgc cgttgtagta
     2281 gcgcgccgcc tcgccgatgg tgaggccgta gatgaacggc gccgggaagg aggcgatgtg
     2341 ccgcgagatg aagcgtcggt ccagcggcgg tccgtccact cccagaaaac ccagcgggtt
     2401 gggccggtcc agcaccacga gctcggtccc ggtgccggca agcgcgtcca tgaccatctt
     2461 gaggtcggag acataggtgt agcagcgcat ccccatgtcc tggatgtcgt agatgacgag
     2521 gtcgacgcgc cgcaggagat gccgaagcga atcgaccgtg agcaggtgca ggttgtacac
     2581 catgaggttc cgccgctcgt cgacgatata gccgcgggtg tcgtaggcgt tctggtgtcc
     2641 gtgcaggccg tgctcgggcg cgaaggccag ggcgatggat atgccggagg cgcgcaggag
     2701 ctccacgttc gcgcgcaggg agtggtccac tccggagtgg ttgctcacca gcgcggcggt
     2761 cctgccgcga tagtctttcg cgcgctcgcc gatgaagacc tcgagtcccg tgagcacgcg
     2821 ccgcttgctt ccggacggca ggtcgtactg gaccggcgcg tggccgcagc cgatcgcgcc
     2881 gcacgcaatc gcaagcgcgg tcgtgagatg aatcacgcgc cgcgggatcg cgcgcaaagg
     2941 tttggtgcat gcatgtgcca tcgcgcggcc acgctaccgc tcggcggcgt aagtatcaaa
     3001 cattttaccc gggatccatc cgataaaaag cttgaaaaat aatgcgcccg ggcgaatgct
     3061 cgtggtcgca ttgatttcaa ggagcgaccg cccatgtatc gagccctgct gttgttgatg
     3121 acgctggtcc aggtggcctc gtgccagacg atgcacgagc gcgagaaggt gatcctcgac
     3181 tactcgcggc aggcgggtgc ggggcgctcg gcctacgagc agtatctggt ccaggaggac
     3241 cagtatacga ccaacggcgt cgtctatcac cggaaatacc gcacctacct cctcggcatc
     3301 gcgcgctaca tgatcgaaga gaagaaatac gcggtcctta agaactgcat cggcttttat
     3361 ttcgacaagc gcgagaacat caaatccagg ctctacctgg ggctggacgt ggagttgccg
     3421 gtggatgcga gcctcgcgtc ctcgagcggg cgcggcattg ccctgagctt cttcaacaag
     3481 cacctgtcgg aggtgctcta tgtgctcttc tcgtgcaaaa ccattttcgc ggaggcggag
     3541 gtggtgggcg cggtggtcgg tttccagtgg cgcgccgccg ggagccagga gatctacaat
     3601 atctggatcg acaaaaaaga cgtaatcaag ttcgaaaacg gctcactcac gctccgcgag
     3661 atcgtggagc gcaacatcat caccgatacg cgcggcaagg tcgtcaagct gtcacaatag
     3721 cgcccagttc cttcacgagc gtcttgatct ccatcatcgt tcccacggtg atgcggacat
     3781 actcgccctg caccggtccc gtaaagtagc gcaccaggat cttacgctcc ttgagctgtt
     3841 cgaagaggtc cgcggcgggc acggacgggt gccggacgaa aagaaagttc gccttggagg
     3901 ggaccacctc gaaatccatg tcggccagcc gctcctccag gtattccttg ttgctgcgaa
     3961 gcatcgagat gttgtaggcg acgcccttgc ggtcgagaat ggccgcgcgc gccgccgcct
     4021 cggccacccg gtcgatgttg tacgagtcct tgagcttgag caggccctgg atgatctcct
     4081 tggacgccgc ggcgaaaccg acgcgcatcc ccgccagcga gcacgacttc gacaacgagc
     4141 gcgtcacgat gatattgtcg aaatcggcga caagcccgat ggcggtctcg ccgtagaaat
     4201 cgacataggc ctcgtccacc accaggagac ccgcgaattt ccccaggaag gcgcggatgg
     4261 ccttcacctc cacgccgcgg ccggtgggat tgttggggtt tccgatgatt accaggtcat
     4321 agggtttttt caggtactcg tccaggttca cgtccagcga cgcggtgagc ggcagttttt
     4381 cgtacttgat gccgttggcc tccgcgagcg tgtagtacag cgaatacgat gggtagggaa
     4441 acgccgcgag gcccttgggc tcgatgaagc cgcgaaacag cagcgtgaag atctcgtccg
     4501 agccgttgcc cagcatcacg ttttccacgc ccagcgcgtg ttccttggcg aaggcctcgc
     4561 gcgcggacag cgaggtgggg ctggggtagc ggcggatgga ctcgtcgcag gccttgcgca
     4621 tcgcctccgt gaccgaggcc ggcgggggga acgggttttc gttggtgttg agcttgatga
     4681 actcgctcag gttctccggc tgctcgccgg ggacgtactc gctcatcttt ttcagtcggg
     4741 cgttccagta tttcatcggt tcctcttgtt atgggcccgt cgtgggcgtg ttgattggct
     4801 gggcggcatc gcacgcgctc gaggccggga cgcggtcccc gtgggcgcac ccgatcaagt
     4861 cgcgcaccgt gccgatccgc tccgcgcggc agaactcttc gatcccctgc aggcagcgga
     4921 tccccgcgtc gggatgcacc atgttcatgg tgccgatgga aaccgcgctc gcgccggcca
     4981 tgatgaactc cagcgcgtcc tcggccgtgg cgatgccgcc gatcccgacg atggggatgc
     5041 gcgtgcgctc gaatatctcg tatacggcgc gcacggccat cggacggatc gccgggccgc
     5101 tcaggcccgc cacgcggttg cggaagtgcg ggcgtcgcgc atcgatgtca atcttcatgc
     5161 ccagataggt gtttacggcc gacaccgcgt cggcgccgcg ctcctcggcg attttcgcga
     5221 actcggtgat gtcggtcacg ttgggggaga gtttcacgat gagcgcgcag cgcgtgacgg
     5281 cgcgcgtttt ttccaccagg tccgcgaaca ccttcgggtc gcgcccgaag gcgatcccgc
     5341 cctccttcac gttggggcac gatacgttga gctcgagggc cgcgacgccg ggggcgttcg
     5401 aaagctcccg gcagaggtcc acgttctcgt ccaccgagtg cccggcgacg ttgggtatca
     5461 ccgcgacccc cgtggccgca aggggcggga gcttgtccga aaggaatttt tcgaggccca
     5521 cgttctccag gccaatggag ttcagcacgc ccgccggcgt ttccaagatg cggttgcccg
     5581 cattgcccgc gcgcggtttc agcgagagcc ccttggtgaa gatggcgccg atgcgcgaga
     5641 gatcgtaata cttcgcgagc tcctccccgt agcccgcggt cccggaggcg agggtgacgg
     5701 gatttgtgag cgtaagcggc ccgatgttaa ccgagaggtc catgtcgata gcgagtgtcg
     5761 cgtgcgcgcc cgtgcgcgga ttcagagaat ttcactttac aggtgcccgc gcgcggcgga
     5821 caagcgtacg gggcggttga cggtgcatac gagcgcgcca aaccgcgcga gtcaagaatt
     5881 aaatgtgcgc agggagcagc catggccgtt ctcgccttcg acgtcgacgg caccatcttc
     5941 gacagcgaca gcattatcgt ggacgccttc gccggcggga tcgacgactt catgcgacgt
     6001 cagccgggcg cacgcctgga tcttcccgca aaatcggaca tcatcgcgca gatcggcaat
     6061 ccggccggcg ccatcttctc ggcgctcttt cccgggctcg atgcggattc gcgatcgctc
     6121 atggagcgat tgtgcaccga acggctctcg gcgatggtgc gatccggcgg aggggggctc
     6181 atcgacgggg ccgcggaaac gctcgccgcc ctggcgcgcg atggccaccg gttcctcgtt
     6241 gcgtcgaacg gccgcatcga gtacctgcgg agcatcctgg agacgcacgg cctctggcgg
     6301 ctcttcgagc ggccggtcct gtgcatcgac cgcgcgtccg gccgccgcga caagacggac
     6361 atcgtgcgcg agtacgtccg gctccattcg tccggcaacg gcgtgttcgt gatgattggc
     6421 gaccgtggat cggacatggc cgccgctcgc ggtaacgggg tcgccttcat cggctgcgcc
     6481 ttcggtcatg cgggcgacgc ggagatctca agcgcccggc atatcgcccg ccgcttcggc
     6541 gagatccccg cgctggtaaa aataatcgag gcgtcgtttc cgacgcctcg aatcatgcac
     6601 tgcggcctct tggagtagct tatctctgcg tctttttaat atcccttgcc cttcagttcg
     6661 gacacgatgc ccacgaagaa gtcgggagcg tcgaagcccg gggtgatccc gaatggctgg
     6721 tcgatgatgt tcaggtggtc gacgccggcc gaataccatg cgccttcatg cgtcccgcgg
     6781 aaggtgcccc actgcgccga ggtgaccgat accagaccgt cgttcgcccc ttcatagtag
     6841 aggaggatcg gccaggtgac caggaagtac cagttgcgcg cgtcgatcgc catcgtcttg
     6901 atcttcgccg cccagctctg atagtagatg ccggacttgt tgggggtgtt cggattaaag
     6961 gtgtttatca tatagctgcg gaccaggtcg tagccgttct gcaggctgtt tgggttcttg
     7021 tcgccgaaga tgaaggcgta cacgaaatcc aaggtcgcgc cgacgagcgg ttgggccacg
     7081 ttggggacaa cgcctaaaat cacgtctgcg accgcgctgc cgcggtgcgg gccggcgatg
     7141 ctggtatggc tggccacata cgaggaaagc gccaggttcg agatcgcata ccgggtgtag
     7201 atggtgccgt gcgagtggcc gatcacgttc agtttcgccg cgccggtgac cgccttgatg
     7261 ttcaggaatt gcgctttaaa actggccgcc ttgttggcgg tgctgtccat gccgttcacc
     7321 gaggtgatgt acaccttcgc tccctcgtcg cggagggcct ggggaatgcc gttccag
//
LOCUS       DAORHF010000187         7362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_9782_length_7362_cov_6.249213, whole genome shotgun sequence.
ACCESSION   DAORHF010000187 DAORHF010000000
VERSION     DAORHF010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7362
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9782_length_7362_cov_6.249213"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PKJ16_13795"
     CDS             complement(<1..400)
                     /locus_tag="PKJ16_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HNV48112.1"
                     /translation="MKRTRIVDILRAAAPGTEVLVKGWVRTRRDAKGFSFLEINDGSS
                     ITNLQVIAAEAMPGYDIVQRITTGCSVAVAGALTESPGTRQALELHAAEITVYGWADP
                     ESYPLQKKRHSFEFLREIAHLRPRTNTFGAV"
     gene            complement(405..3035)
                     /gene="alaS"
                     /locus_tag="PKJ16_13800"
     CDS             complement(405..3035)
                     /gene="alaS"
                     /locus_tag="PKJ16_13800"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HNV48113.1"
                     /translation="MMHVRDLRDSFIQFFREREHRIVPSSPLIPRDDPTLLFTTAGMV
                     QFKPMFAGTVALDYTRAASVQKCFRTSDLENVGNTKRHCTFFEMLGNFSFGDYFKNEA
                     IAYAWDYSTKVIGFDKKDIWVSIYEADDEAFAIWNKSIGVPAEKIVRLGKKDNFWGPA
                     GDSGACGPCSELYLDRGPAFGCGSPDCKPGCECERFLEYWNLVFNQYFQDTAGNLSPL
                     PKTGIDTGMGLERLATLVQNVDSIYHTDELKGFVDFVSRETKTPYEGKNIVPINVLVE
                     HARALTFAMSDGAYPSNEGRGYVLRRILRRALRFGRQIGVTEPFVFRMVDPIVAAMAP
                     YYPEIKPAAKNVKAVIEGEERRFLETLDNGMDRLDEIITSLKKSGVREISGRDAFLLY
                     DTFGFPVEMTVEIAREEKLGVDLVGFEREMNDQRERGKKSWKGIDSSMLAVMDELSKR
                     AGATEFRGYDADVTESVVTLVADANGVVDSIAEGMDGMIVAAATSFYGESGGQVGDRG
                     VITTAGGARFAVEDTRKINSTVVHVGRMERGSLTAGDAITTSIDSVRRNLTRANHTAT
                     HLLQAALKSVLGEHVKQAGSQVDPERLRFDFSHFQAVTAEELAKIEEIVNGKIWENAP
                     VETIERALEEAIAMGATAVFDEKYESRVRVVSVPGFSMELCGGTHVDNTGKIGVFTVL
                     RESSPGAGMRRIEALTLKGLLDRYRAQHEIIRELTDDLKVPEQNLPRRVGDLVERAGA
                     LEKELKKARTKGAGTDINALIASGTDCGGVKLISHTAADLSIDELRSLADAIRAKEQS
                     CAVILGASADGKVHLLCAATKSAVAKGIDCGALVKEAATIVGGGGGGRKDMAQAGGSI
                     PEKLPAAIEAAVARAKSTLA"
     gene            3193..5262
                     /locus_tag="PKJ16_13805"
     CDS             3193..5262
                     /locus_tag="PKJ16_13805"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV48114.1"
                     /translation="MGLRSLLPLVAALIVLSPAYGAPTERDMLVTVAPQSDMLLVGGA
                     ARVLADPDGALTPAEALASKNWRTNDGPFIRFGYTRAVVWVRWDILNAAGTERYLEIP
                     QPATDRIDLSVFEAGAERATRKSVGDWFPFRNREIDHRNYVFSVPPRGVPATYLFRIS
                     STSYITFRPVLWAGDAFARHDRFDLALIWAFFGIMIVMALYNLIIFVSVREGAYLFYV
                     LLCLGYAVHIIACRGLGFQYLWPESPVWANIAPATFLIVIALMIMQFAVYFLRLKTEA
                     RRLYVAINIATAVILVNLVMTTVFHVGTFSNVGFLAAIGASFVISVYAVYRAAKGSRP
                     ALFYSAAWIWMFAMIIIGSLYTSGGLHIPFVSAWSIQIGGFVQTVLLSIGLADRINSM
                     RRELIVLNTGLERKVMERTEELQAAMDELTALNENLADTNQRLEEAHRIARRDMDMAV
                     TVQANFLPQEPPGSPEWECAFFFRPMAGVSGDFYDFYVADGRFAGVGIFDVSGHGIAS
                     GLVTMIAKSVILRAFAEGIGRGLNEVLENANVRLQTEIGGIDNYIAGSLLRFADGEVE
                     FVNAGHTDVFRRSKGAVAPVRKADDSTLDGVFLGLSGFSYPYEVLRFAVDSGDCLLLY
                     TDGLVEAEGPRQERYGHGRIMRSLANAPAEGAQAVRDAVVEDFFSFVGRDAALRDDVT
                     VMVLRRR"
     gene            5262..5783
                     /locus_tag="PKJ16_13810"
     CDS             5262..5783
                     /locus_tag="PKJ16_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48115.1"
                     /translation="MRGPDSGTICQPGRGASCALCCGSHNYRAGRDEMDALFRARRRA
                     FPATVPSPLPMVIDDGIQCPYVGFIDEADSIIGCLLYFDESSASGDHRAFMARTCRVF
                     SCMARETLSPDEVRFAAALMGDWLHYGLLINDIALLRDLMRRFALPAHVTSEEREAVR
                     ERLSRAAFSPKMC"
     gene            5825..7141
                     /locus_tag="PKJ16_13815"
     CDS             5825..7141
                     /locus_tag="PKJ16_13815"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HNV48116.1"
                     /translation="MEPIVEHSRPYRILYFCFFGFLITSFFSISLNQGFLALCVAVWL
                     YGLIRKTPDISFYRSGLERHIAVFVGVSVFVAFLSPMVVQNLQWMGDHLLAAAFFVTA
                     SVVSTREDLARVIKALVVIAIVSSVAGIVQLATGIDFSATWVRFAKTGSLAPVYGKDL
                     VEGLLGLKLSYGGYMMTIMLPLAYLSFARPDEVRGWSRLPVRVGAVLSFVAVVISWTR
                     SALVALPAAAFPLFFKRKKLFVAIMLIIIVVVMLVLSMFGQGSAKRIIGPDGVNVHAD
                     SVRDFFVWTLNLGSPGRVLIWQTATRVWLKHPVFGAGGAHYYEVYKEVMAEHPDEQPP
                     MISHAHNDYLNMLARKGIVGFAAFIYLLAGLFRYMVVNLRDIRDRAARLLYMGLFASL
                     CCFCAAMLFQCYFSDDKNVVMFWFVAGLLAAIVKIERAEREAEGAS"
     gene            7138..>7362
                     /locus_tag="PKJ16_13820"
     CDS             7138..>7362
                     /locus_tag="PKJ16_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48117.1"
                     /translation="MSPLLWHKLQLFAASFAVSVVAVPLIMKLSETFGLYDRIDERKI
                     HKKKISRLGGLGIFLGFIMPFLYTVFSVANP"
BASE COUNT         1122 a         2367 c         2386 g         1487 t
ORIGIN      
        1 gcaccgcccc gaaggtgttg gtgcgcggcc gcaggtgcgc gatctcccgc aggaactcga
       61 atgagtggcg cttcttctgg agcgggtagg actcgggatc ggcccagccg tatacggtga
      121 tctccgctgc gtggagctcc agcgcctgcc tcgtgcccgg ggactccgtg agcgcgccgg
      181 ccacggcgac gctgcagccg gtggtgatgc gttggacgat atcgtagccg ggcatggcct
      241 ccgcggcgat cacctgcagg ttcgtgatcg acgagccgtc gtttatctca agaaaggaga
      301 agcccttcgc gtcgcgccgc gtgcgcaccc agcccttcac caggacctcg gtcccggggg
      361 cggccgcgcg caggatgtcg acgatgcggg tgcgcttcat cgctctaggc cagcgtcgat
      421 ttcgcgcggg cgacggccgc ctcgatcgcc gcggggagtt tttcgggtat cgatccgccg
      481 gcctgcgcca tgtccttgcg cccgcccccg ccgccgccca cgatcgtcgc ggcctccttg
      541 accagcgcgc cgcagtcgat ccccttcgcg accgcgctct tcgtggcggc gcagagaaga
      601 tgcaccttgc cgtcggcgga tgcgccgagt atgacggcgc agctctgctc cttcgcgcgg
      661 atggcgtccg cgagcgagcg gagctcgtcg atcgagaggt ccgccgcggt atgggagatg
      721 agtttgacgc cgccgcaatc ggtcccggac gcgatcaggg cgttgatgtc ggtccccgcg
      781 cccttcgtgc gcgctttctt gagctccttc tccagcgcgc ccgcgcgctc gacgagatcg
      841 ccgacgcggc ggggcaggtt ctgctcgggc accttgaggt cgtcggtgag ctcgcgtatg
      901 atttcgtgct gcgcgcgata gcggtccaag agccccttga gcgtgagcgc ctcgatgcgg
      961 cgcatgcccg cgccgggcga gctctcgcgc agcaccgtga acacgccgat cttcccggta
     1021 ttgtccacgt gggtgccgcc gcagagctcc atgctgaaac cgggcacgct caccacgcgc
     1081 acgcgcgact cgtatttctc gtcgaagacg gcggtcgcgc ccatcgcgat ggcctcctcc
     1141 agcgcgcgct caatcgtctc caccggggcg ttttcccata tcttgccgtt tacgatctct
     1201 tcgatcttcg ccagctcctc ggcggtcacg gcctggaagt gcgaaaagtc gaaacgcagg
     1261 cgctccggat cgacctgcga tccggcctgc ttcacgtgct cgccgagcac cgacttgagc
     1321 gcggcctgca gcaggtgcgt ggcggtgtga ttcgcgcgcg tgaggttgcg tcgcacgctg
     1381 tcgatcgacg tcgtgatcgc gtctccggcc gtgagcgacc cgcgctccat ccggccgacg
     1441 tggacgacgg tgctgttgat cttgcgggtg tcctcgacgg caaagcgcgc gccgccggcg
     1501 gtggtgatga cgccgcggtc gccgacctgt ccgccggact cgccgtagaa cgatgtggcc
     1561 gcggcgacga tcatcccgtc catgccctcg gcgatcgaat cgacgacgcc gttcgcgtcc
     1621 gcgaccagcg tgacgacgct ctctgtgacg tcggcgtcgt agccgcggaa ttcggtcgcg
     1681 ccggcccgct tcgaaagctc gtccatgacg gcgagcatcg aggaatcgat gcccttccag
     1741 cttttcttgc cgcgctcgcg ctggtcgttc atctcccgct cgaacccgac gaggtccacg
     1801 ccgagcttct cctcccgggc gatctcgacg gtcatctcca cggggaagcc gaaggtgtcg
     1861 tagagaagga aggcgtcgcg tccggagatc tcgcgcacgc cggatttttt cagggatgtg
     1921 atgatctcgt cgagccggtc catgccgttg tcgagcgtct cgaggaagcg cctctcctcg
     1981 ccctcgatga cggccttcac gtttttcgca gccggcttga tctccgggta gtacggcgcc
     2041 atcgccgcga cgatgggatc gaccatccgg aatacgaacg gctcggtgac gccgatctgc
     2101 cgcccgaagc gaagcgcgcg ccggaggatg cgccgcagga catagccgcg tccttcgttg
     2161 gaggggtagg cgccgtcgga catcgcgaag gtgagcgcgc gggcgtgctc cacgagcacg
     2221 ttgatgggca cgatgttctt cccctcgtac ggggtcttcg tctcgcgcga gacgaaatcg
     2281 acaaagccct tcagctcgtc ggtgtgatag atcgaatcga cgttttgcac gagcgtggcc
     2341 aggcgctcaa gccccatccc cgtgtcgatg ccggtctttg gaagcgggga cagattcccg
     2401 gcggtgtcct ggaagtactg gttgaagacg aggttccagt actcgagaaa gcgctcgcac
     2461 tcgcacccgg gcttgcagtc gggcgagccg cagccgaagg ccggcccgcg gtcgaggtag
     2521 agctcggagc acgggccgca cgcgcccgaa tcgcccgccg ggccccagaa gttgtccttc
     2581 ttgcccaggc gcacgatctt ctcggcggga acgccgatgg acttattcca gatggcgaag
     2641 gcctcgtcgt cggcctcgta gatggacacc cagatgtcct tcttgtcgaa gccgatcacc
     2701 ttcgtggaat agtcccaggc gtaggcgatc gcttcgttct tgaagtaatc gccgaaggag
     2761 aagttgccca gcatctcgaa gaaggtgcag tggcgcttgg tgtttcccac gttctccagg
     2821 tcgctggtcc ggaagcattt ttggacggag gccgctcgcg tgtagtcgag cgcgacggtc
     2881 ccggcgaaca tgggcttgaa ctgcaccatg ccggccgtgg tgaacagcag cgtgggatcg
     2941 tcccggggga tgagcggact cgagggaacg atgcgatgct cgcgctcccg gaagaactgg
     3001 atgaatgaat cgcgaagatc cctgacgtgc atcatggccg acctcgtggc tgcggaaata
     3061 ctgccggaaa aatttggttg aaatgttcgg cgtgaccggg cagggtcccg gatcatgccc
     3121 ggaaaaccga tgtttattca acgatcgacc cgatgaacgt caatagaaaa aatagaaaat
     3181 ttcggggtcc gaatgggatt gcgtagcctc ctgccgctcg tcgcggcgct catcgtgctt
     3241 tcgcccgcgt acggcgcgcc gacggagcgc gacatgctgg tcacggttgc cccgcagtcg
     3301 gacatgctcc tcgtgggcgg cgcggcgcgg gtccttgcgg acccggatgg cgcgctcacg
     3361 cccgccgagg cgctcgcgtc gaaaaactgg cggaccaatg acgggccctt catccgtttc
     3421 ggctacaccc gcgcggtggt ctgggtccgc tgggacatcc tgaacgccgc cgggaccgaa
     3481 cgctatcttg agatacccca gccggcgacc gaccgcatcg atctctccgt cttcgaggcg
     3541 ggcgccgaac gcgcgaccag gaaaagcgtg ggcgactggt tcccgttccg taatcgcgag
     3601 atcgatcacc ggaactacgt cttctcggtg ccgccgcggg gcgtgccggc cacctatctg
     3661 ttccgcatca gctcgaccag ctatatcacc ttccgtccgg tgctgtgggc cggggacgcc
     3721 ttcgcgcgcc atgatcgctt cgatttggcg ctcatctggg cgttcttcgg catcatgatc
     3781 gtgatggcgc tctacaacct catcattttc gtctcggtcc gggagggcgc ctatctgttc
     3841 tatgtgctcc tgtgtctcgg gtatgccgtc cacatcatcg cctgccgcgg cctgggcttc
     3901 cagtatctct ggcccgaatc gccggtctgg gcgaacatcg cgcccgccac ctttctcatc
     3961 gtcattgcgc tgatgatcat gcagttcgcg gtctattttc ttcgcctcaa aaccgaagcg
     4021 cggcgcctgt atgtcgccat caacatcgcg acggccgtga tcctggtcaa cctcgtgatg
     4081 accaccgtgt tccatgtcgg caccttcagc aatgtggggt ttctcgcggc gattggcgcc
     4141 tcgttcgtca tctcggtcta tgcggtctat cgcgcggcga agggatcgcg cccggcgctc
     4201 ttctacagcg ccgcgtggat atggatgttc gcgatgatca tcatcggcag cctgtacacc
     4261 tccggcggac tgcacatccc cttcgtgagc gcgtggtcga tccagatcgg cggtttcgtg
     4321 cagacggtgc tgctttcaat cggccttgcg gaccgcatca attcgatgcg gcgcgagctc
     4381 atcgtcctca acaccggact ggagcgcaag gtcatggagc ggacggagga gctccaggcg
     4441 gcgatggacg agctcacggc gctcaacgaa aacctggccg acacgaacca gcgcctggag
     4501 gaggcgcacc ggatcgcgcg gcgcgacatg gacatggccg tgaccgtgca ggccaatttc
     4561 cttccgcagg agccgcccgg gtccccggag tgggagtgcg cgtttttttt caggccgatg
     4621 gcgggcgtgt ccggcgattt ttacgatttc tacgtggcgg acggccggtt cgcgggcgtc
     4681 ggcatcttcg acgtttccgg ccacggtatc gcctcgggcc tggtgaccat gatcgcgaaa
     4741 tcggtcatcc tgcgcgcgtt tgccgagggc atcggccggg gcctgaacga ggtgctggag
     4801 aacgccaatg tccgtctcca gacggagatc ggcgggatcg acaactatat cgccgggtcg
     4861 ctcctgcgct ttgcggacgg ggaggtcgag ttcgtcaatg cgggacacac ggacgttttt
     4921 cgacgctcga agggtgcggt cgccccggtg cgaaaggccg acgactcgac gctcgatggc
     4981 gtcttcctgg gattgagcgg gttctcctat ccgtatgagg tcctccgctt cgcggttgat
     5041 tcgggcgatt gcctgctcct gtacacggac ggactcgtcg aggctgaggg tccgcgccag
     5101 gagcgctatg gacacggccg catcatgcgg tccctcgcga atgcgccggc ggaaggagcg
     5161 caggcggtcc gtgacgcggt tgtcgaggat ttcttctcgt tcgtcggtcg cgacgcggcg
     5221 ctccgcgacg atgtcaccgt catggtgttg cggcggcgat gatgcgcggg cccgacagcg
     5281 gcaccatctg ccagccgggg cgcggcgcgt cgtgcgccct gtgctgcggg agccacaact
     5341 atcgcgcggg ccgcgacgag atggacgcgc tcttccgtgc gcggcggcgc gcctttccgg
     5401 cgacggtccc ctcgccgctg cccatggtga tcgacgacgg tatccagtgt ccctacgtcg
     5461 ggtttatcga cgaggccgac tcgatcatcg gctgtctgct ctatttcgac gagtcgtccg
     5521 cgtccggcga ccaccgcgcc ttcatggcgc ggacctgccg cgtgttctcc tgcatggcgc
     5581 gcgagacgct ctcgcccgac gaggtgcgct tcgccgccgc gctgatgggc gactggcttc
     5641 attacggcct gctcatcaac gacatcgccc tgctgcgcga cttgatgcgc cgcttcgcgc
     5701 tgccggcgca tgtgaccagc gaggaacgcg aagctgtgcg cgagcgtctc tctcgcgccg
     5761 cgttttcgcc aaaaatgtgt tgacggatat ggaaaacccg ccaatacatc ggcacggaag
     5821 ccccatggag ccaattgttg aacatagccg gccgtaccgg atcctctatt tctgtttctt
     5881 cggatttctg atcacgtcgt tcttttccat atcgctcaat cagggctttc tcgccctgtg
     5941 cgtcgcggtg tggctgtacg gcctcatcag gaaaacgccg gatatctcat tctatcggag
     6001 cggtctcgag cggcacatcg ccgtgttcgt gggcgtgtcg gttttcgtcg ccttcctttc
     6061 tccgatggtc gtccagaacc tgcaatggat gggagaccac ctgctcgcgg cggccttctt
     6121 cgtcaccgca tcggtcgtct cgacgcggga ggacctcgcg cgcgtgatca aggcgctcgt
     6181 cgtcatcgcc atagtgagct ccgtcgcggg catcgtgcag ctggcgacgg gcatcgactt
     6241 ctcggcgacc tgggttcgct tcgcgaaaac cggaagcctc gcccccgtgt acggcaagga
     6301 cctcgtcgag gggctcctgg gcctcaagct ctcctacggt ggttacatga tgacgatcat
     6361 gctgccgctg gcgtacctct ccttcgcgcg tcccgacgag gtccgcggct ggtcgcgatt
     6421 gccggtccgc gtcggcgcgg tcctttcgtt cgtcgcggtg gtcatcagct ggacgcgatc
     6481 ggcgctcgtg gcgctgcccg cggcggcctt cccgcttttt ttcaaacgaa aaaagctctt
     6541 cgtcgcgatc atgcttatca tcatcgtcgt ggtgatgctg gtattgtcca tgttcggcca
     6601 ggggagcgcg aagcggatca tcggccccga tggggtgaac gtccatgccg attccgtgcg
     6661 cgatttcttc gtctggacgc tcaacctggg gtcgcccggc cgcgtgctca tctggcagac
     6721 ggcgacgcgc gtctggctca agcatcccgt gttcggcgcg ggcggcgcgc actactacga
     6781 ggtgtacaaa gaggtgatgg ccgagcatcc cgacgagcag ccgccgatga tatcgcacgc
     6841 gcacaacgat tacctcaaca tgctggcgcg caaggggatc gtcggattcg cggcgttcat
     6901 ctatctgctc gccggtctct tccgatacat ggtcgtgaac ctgcgtgaca tccgcgaccg
     6961 cgccgcgcga ctgctctaca tggggctctt cgcgtcgttg tgctgcttct gcgcggcgat
     7021 gctgttccag tgctattttt ccgacgataa aaatgtggtc atgttctggt tcgtcgcggg
     7081 cttgctcgcc gccatcgtca agatcgagcg ggccgagcgc gaggccgagg gtgcgtcatg
     7141 agcccattgc tgtggcacaa gctccagctc tttgcggcgt ccttcgccgt ctcggtggtg
     7201 gccgtgccgc tcatcatgaa gctgtcggag accttcgggc tctatgaccg catcgacgag
     7261 cgcaagatcc ataaaaagaa gatcagccgg ctcggggggc tggggatatt cctgggcttc
     7321 atcatgccgt tcctctatac ggtgttctcg gtggcgaacc cg
//
LOCUS       DAORHF010000188         7224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10078_length_7224_cov_4.554052, whole genome shotgun sequence.
ACCESSION   DAORHF010000188 DAORHF010000000
VERSION     DAORHF010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10078_length_7224_cov_4.554052"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PKJ16_13825"
     CDS             complement(<1..764)
                     /locus_tag="PKJ16_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HNV48118.1"
                     /translation="MGKKFIVSIDLGTTGNRVFCFDETGQPISSAYREFTQHFPRPGW
                     VEHDAIEIWNYIKQLIPEAIQKGGLDGADAISIGITNQRETTVIWNKDTGMPIHNAIV
                     WQCRRTTDICNTLKEQGHEDIFRRKTGLVIDAYFSGTKIKWLLDTVSGARDLAKQGKL
                     LFGTIDTWILWNLTAGKSHKTDYTNASRTLIFNIVDKRWSRELLEILDIPLNILPEVR
                     DSASFFGETMGVPGLPDGVTIGGIAGERHEEAGLGR"
     gene            930..1979
                     /locus_tag="PKJ16_13830"
     CDS             930..1979
                     /locus_tag="PKJ16_13830"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNV48119.1"
                     /translation="MKLAGLFPGKNILCIAEIGINHNGDCDMAERMVRAAALAGAHMV
                     KFQTYVPELMYSPYTTSLLASGREGDADFSQIEFFRRFVLAKEDYVRLRDVARECGVE
                     FFSSAFDEPSVDLLEEVGVRLYKVASSEVGNLRLLSRVARTSKPVIMSTGICTEDEIA
                     RALDLVRAEGCPDIALLHCVSNYPLDAAHANLRRISQLAERFGAPVGISDHSSGTDLV
                     GAAVALGARIFEKHFVHDPGYECPDRNVSVAPAEFAAMRQAADNALAALGDGRIAMGD
                     SEKEVALSARRSLCAGRDIPAGKRLEAADVVPLRPGVGMPVNRCAGMLGRVSRRLIPK
                     GHLIREEDFEEGICE"
     gene            1976..2968
                     /locus_tag="PKJ16_13835"
     CDS             1976..2968
                     /locus_tag="PKJ16_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48120.1"
                     /translation="MKSVRMGLIAALMCAALPFLMGTVFLPEIESEGFFTAEETSVIR
                     DLCRAMSLKHGYSFELELDYISPDGKAQAALTSADPRFYEVVDASNIEVSKTLFIKLY
                     RLYRQTECQVNRYSYKDPRNFKRYSALYLESIKTVAIGMEDALCKKDVFLRADFQARK
                     AAIDREEALACFLTEEEGEFFNSADREILDATLHAIDYEHGYDSEIQLAYIFAFSHSE
                     TITPPREKEFHKVVSKVDPVKLLSFYEKLFKLVKITEKKTREYHEQKKWVLYTYLSKK
                     LTPSLTTYFALFEKSVLLAQPKAKGELAERQGRVMAWLNLEYRFRQRIVETFEF"
     gene            2994..4124
                     /locus_tag="PKJ16_13840"
     CDS             2994..4124
                     /locus_tag="PKJ16_13840"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV48121.1"
                     /translation="MNAIEDIRRVARDPIEYARRYTNETGVRALGYFCSYTPEEIIHA
                     AGILPVRLFGFGKKIVRADAHLQSYSCSLVRSALELGLSGELDFLGGMVFPHTCDSIQ
                     RLSDIYRMNIPFGFFEDIVLPVKLNSESAREYIVNLLAAFTASIERGFSVTVSDEALR
                     RSLVLYNEIRDHLGALYRLRSRHPRLCSGADLHDVVSASMVMDRTGLAALLADLVGEL
                     SARAEGLGDDDARKRIILSGGICSHPDVYGIIEDAGGAVVWDDLCTGTRYFEGALDTT
                     RPPLEAIAHRYVDTRVTCPAKHRSLTDRADSLAAMATEHRADGVVFLLLKFCDPHAFD
                     YPHLKERLDREGVPSMLVEIEDTLPPAGQLMTRFEAFVESLG"
     gene            4168..5418
                     /locus_tag="PKJ16_13845"
     CDS             4168..5418
                     /locus_tag="PKJ16_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HNV48122.1"
                     /translation="METIEKKPDSEKERRKIKSVARMKEIMTGYYIEAKGARQSGKKV
                     AWITSGGPVEPLIVMDVIPVYPENHSAMIGATKMGGDLCNRAEEMGYSRDLCSYARSD
                     IAAAVTNGGPIGGLPAPDFLVCCNNICGTVMKWYEAQARFFNVPLFIFDTPFTHTEFP
                     DIARNYVRKQIFEYIAFLETVCGRKFDYDRMMEVGRLSVEAQRLWQAVLDTTMHRPAP
                     MSAFDAFFHLGLIVTLRGTQVVVEYYTQLLAEMRARVASGISAVPNERYRLLWDNLPV
                     WYKTKWLSETFAAHDACLVADTYTSAWSGQVEKLREDDFLESMVEGYSTIYLNVSVAE
                     MARSVLGMIDKYGADGVVMHSNRSCKPYSLGQYDIQRIIQKERDVPVLMIEADMVDER
                     VFSESQIGTRIDAFMEVVRSRKSG"
     gene            complement(5419..5739)
                     /locus_tag="PKJ16_13850"
     CDS             complement(5419..5739)
                     /locus_tag="PKJ16_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48123.1"
                     /translation="MKSVKELIRSHDAYIDASRGDPGFPARHATRIAMLQHERLAHLI
                     TMFAVIIIDVLFLGLYLQHDSVLLCAVFGILCALSTFYVAHYYTLENTLIRWYGVSGT
                     DDGK"
     gene            5841..6173
                     /locus_tag="PKJ16_13855"
     CDS             5841..6173
                     /locus_tag="PKJ16_13855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 domain-containing protein"
                     /protein_id="HNV48124.1"
                     /translation="MIPDTIEKIEKKINGSRTISDEKKRELLKLVDELKREVLAASGD
                     DRDGAQSVANFANASAHEALREIVDPRMFELARNGLSESVRRFEVTNPGLVSVVNALS
                     TVLANMGI"
     gene            6190..6630
                     /locus_tag="PKJ16_13860"
     CDS             6190..6630
                     /locus_tag="PKJ16_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48125.1"
                     /translation="MDLQQYVYERTEGLQNGQRSLYGFSREGSFKKWVLGIERISFAS
                     PFSEWIFVTVPTTESGVSPEHADEQPYGWNGHINDYAAESAVWYAFDLLTETEAQEFM
                     ENHRPEVIFEFVERGHPERLQVRYENGRWIVVDEIEEIGGIGAR"
     gene            complement(6627..7178)
                     /locus_tag="PKJ16_13865"
     CDS             complement(6627..7178)
                     /locus_tag="PKJ16_13865"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV48126.1"
                     /translation="MIERQGILGAVKFMAGFVLLLWIILLASAAFPIKNLGIVPRTIT
                     GLVGVAAAPFLHQDAAHLIANSFSLFVLGTVLIALEKGRALTIMISLAILGGFGTWLI
                     GRPGTVHIGASGLVFGLLSYLFFFGVFKRDLKSIIVSIAIFFLYGGAVWGVIPSSPHV
                     SWESHLCGFIAGIPLARYYARRA"
BASE COUNT         1432 a         2085 c         2329 g         1378 t
ORIGIN      
        1 caccgcccaa gtccggcttc ttcgtgccgc tccccggcga tgccgccgat ggttacgccg
       61 tcgggaagac ccggcacgcc catcgtttcg ccgaagaacg acgccgagtc gcggacctcg
      121 ggaaggatat tgagcgggat gtccaggatc tcaaggagtt cccgcgacca gcgcttgtcg
      181 acaatgttga agatgagcgt ccgcgaggcg ttggtgtagt ccgtcttgtg cgacttcccc
      241 gcggtcaggt tccagagtat ccaggtgtcg atggtcccga agaggagctt tccctgcttg
      301 gcgaggtcgc gcgcgcccga caccgtgtcg agcaaccatt tgatttttgt gcccgagaaa
      361 taggcgtcga tcaccaggcc ggtcttgcgc cggaagatgt cctcgtgtcc ctgttccttg
      421 agcgtgttgc agatgtcggt ggtgcgccgg cactgccaca cgatggcgtt gtggatgggc
      481 atcccggtgt ccttgttcca gatgaccgtc gtctcgcgct gattggtgat gccgatggag
      541 atcgcatccg cgccatccag gccgcctttt tggatggcct cggggatcag ctgcttgatg
      601 tagttccaga tctcaatggc gtcgtgctcg acccagccgg gccgggggaa atgctgggtg
      661 aattcgcggt aggcgctgga gatcggttgg cccgtctcgt cgaagcagaa cacgcggttg
      721 ccggtcgttc cgagatcgat ggatacgatg aatttctttc ccatggggtc gtcctcgctg
      781 atgatagtgc ggtcgtcgcg tcggggacga tggtagcgcg ataaaatggg gggctgtcaa
      841 atagttttat gcgcgaggta ttattttatt gaaaaaatat caggcgggcc gaatcgtaac
      901 aaaatcaaat cgaaggagcc aaaggataga tgaagcttgc tggactattc cccgggaaaa
      961 acatcctttg catcgcggag atcggcatca accacaatgg cgattgcgat atggccgagc
     1021 gcatggtgcg cgccgctgcg ttggccgggg cccacatggt caagttccag acctacgtgc
     1081 cggagctcat gtactcgccc tatacgacgt cgcttctggc gagcggacgc gagggcgatg
     1141 cggatttctc ccagatcgaa ttcttccgcc ggttcgtcct tgcgaaggag gactatgtcc
     1201 gattgcgcga cgtcgcccgg gaatgcggcg tcgagttctt ctcgagcgct ttcgacgagc
     1261 cgtcggtgga cctcctggag gaggtcgggg tccgactgta caaggtcgcc tcgtccgagg
     1321 tggggaacct ccggctcctt tcgcgcgtgg cccggacctc aaagccggtg atcatgtcca
     1381 ccggcatctg cacggaggat gagatcgcgc gcgccctgga ccttgtacgc gccgaagggt
     1441 gtccggacat cgcgctgctt cactgcgtat cgaactatcc gctcgacgcg gcccacgcga
     1501 acctcaggcg tatatcgcag ctggccgagc gtttcggcgc gccggtggga atctccgatc
     1561 attcatccgg gaccgatctg gtgggggcag ccgtcgcgct gggggcgcga atcttcgaaa
     1621 agcattttgt gcatgatccg ggctacgagt gtcccgatcg aaacgtgtcg gttgcgcccg
     1681 cggagttcgc cgcaatgcgc caggcggcgg acaatgcgct cgccgctctg ggcgacggcc
     1741 ggatcgcgat gggagattcc gaaaaagagg ttgcattatc ggcgcgcagg agcttgtgtg
     1801 ccgggaggga catcccggcc gggaagcgcc tcgaggccgc cgatgtcgtg ccgcttcgtc
     1861 cgggcgtggg catgccggtc aatcgttgtg cggggatgct ggggcgggtc agccggcggt
     1921 tgatcccgaa gggccacctc atccgcgaag aagatttcga agaggggata tgcgagtgaa
     1981 gagcgtacga atgggcctca tcgccgcgct gatgtgcgcg gccctgccgt ttctcatggg
     2041 caccgttttt cttcccgaga tcgaaagcga gggctttttt accgccgaag agacctcggt
     2101 catccgagac ctctgccggg cgatgtcact gaagcacgga tacagcttcg agctggagct
     2161 cgactatatc agccctgacg gaaaggcgca ggccgccctg acgtcggccg atccgcggtt
     2221 ctatgaggtg gtcgacgcca gcaatatcga ggtgtcgaaa acgcttttca tcaagctcta
     2281 tcggctctat cggcagacgg agtgccaggt gaaccgctat tcgtacaagg acccgcgcaa
     2341 cttcaagcgg tattccgcgc tctatctcga atcgataaaa accgtcgcga tcggaatgga
     2401 ggacgcgctg tgcaaaaagg acgtcttcct gcgggccgac ttccaggcgc gcaaggccgc
     2461 catcgaccgg gaggaggcgc tggcctgctt tctcaccgag gaggagggcg agtttttcaa
     2521 ttccgccgac cgtgaaatcc tcgatgcgac gctccacgcg atcgactacg agcacggcta
     2581 cgacagcgag atccagctgg cgtatatctt cgcgttttcg cacagcgaga cgataacccc
     2641 gccgcgggaa aaggagtttc acaaggtcgt gtcgaaagtc gatccggtga agctgttgtc
     2701 attttatgaa aaactgttca agctcgtgaa aatcaccgag aaaaagaccc gtgaatacca
     2761 tgagcaaaaa aagtgggtcc tgtacacgta tctcagcaaa aaactgactc cgtccctgac
     2821 gacgtacttt gcgctgttcg aaaagagcgt gctgctcgcg caacccaagg cgaagggcga
     2881 gctcgccgag cgtcagggca gggtgatggc atggctcaat ctcgagtatc gctttcggca
     2941 gcgtatcgtc gagaccttcg aattctaggc ggcggtacgg ccgggggatg ctgatgaatg
     3001 cgattgaaga catccgtagg gtcgcgcggg acccgatcga atatgcgcgg cgatacacga
     3061 atgaaacggg cgtccgcgcg ctgggatact tctgctcata cacgcccgag gagatcatcc
     3121 acgcggcggg gatccttccg gtgcgcctgt tcgggttcgg gaaaaagatc gtccgggccg
     3181 acgcccacct gcaatcctac tcgtgctcgc tggtccgaag cgcgctggag ctcgggctgt
     3241 ccggcgaact ggactttctg gggggcatgg tcttcccgca cacctgcgac tcgattcagc
     3301 gcctgtccga tatctaccgc atgaacatcc cgtttggctt cttcgaggac atcgtgctgc
     3361 ccgtgaagct caactccgaa agcgcgcggg agtatattgt caatttgctc gcggcgttta
     3421 ccgcgagcat cgagcgcggt ttttccgtga cggtttccga tgaggcgctg cggcgatcgc
     3481 ttgtcctgta caacgagata cgggatcatc tgggcgcgct ctatcggttg cgcagccgac
     3541 acccgcggct gtgctcgggg gccgacctcc atgatgtcgt gagcgcttcg atggtcatgg
     3601 accgcaccgg gctggccgcg ctccttgcgg acctggtggg cgagttgtcc gcgcgcgcgg
     3661 agggactggg cgatgatgac gcgcggaagc ggatcatact ctcgggcggt atatgcagcc
     3721 acccggacgt gtacgggatc atcgaggacg ccgggggcgc ggttgtctgg gacgatctgt
     3781 gcacggggac gcgctatttc gagggggcgc tcgatacgac gaggcccccg ctcgaggcga
     3841 tcgcgcatcg gtacgtcgat acgcgcgtga cgtgcccggc gaagcaccgc tccctcaccg
     3901 accgcgccga ttcgctcgcg gccatggcga cggagcatcg cgcggacggg gtcgtgtttc
     3961 tgctgctcaa attttgcgat ccgcacgcgt tcgactatcc ccacctcaag gagcgtctcg
     4021 accgggaggg cgtcccgagc atgctcgtgg agatcgagga cacgctgccg cccgcggggc
     4081 agttgatgac gcgcttcgag gcatttgtgg aatcgctggg ctgatcggac agcacgcatc
     4141 ggccccaact cacacaggag cgccttcgtg gaaaccattg aaaaaaaacc ggactcggaa
     4201 aaggaacgac gaaaaataaa atcggtcgcc cgcatgaagg aaatcatgac gggctactat
     4261 atcgaggcga agggcgcccg ccagtccggt aagaaggtcg cctggatcac cagcggcggt
     4321 cccgtcgagc cgctgatcgt gatggacgtg atccccgtgt accccgaaaa tcacagcgcg
     4381 atgatcggcg cgaccaagat gggcggcgat ctgtgcaatc gggccgagga aatgggctat
     4441 tcgcgcgacc tgtgctcgta cgcgcgctcc gatatcgccg cggccgtgac caacggcggg
     4501 cccatcggcg gcctgccggc gccggatttc ctggtgtgct gcaacaatat ctgcgggacc
     4561 gtcatgaaat ggtacgaggc gcaggcgcgc tttttcaacg tgccgctgtt tatcttcgat
     4621 acgccgttca cgcacactga gtttcccgat atcgcgcgca actacgtgcg aaagcagatc
     4681 ttcgaatata tcgccttcct cgaaacggta tgcggccgga agttcgatta cgatcgcatg
     4741 atggaggtgg gccgcctctc ggtcgaggcg cagcgcctct ggcaggccgt cctggacacc
     4801 acgatgcacc ggcccgcgcc tatgagcgca ttcgacgcct tcttccatct cgggctcatc
     4861 gttaccctcc gggggacgca ggtggtggtc gagtactaca cgcagctcct cgcggagatg
     4921 cgcgcgcgcg tcgcgagcgg catcagcgcc gtccccaacg agcgctaccg gctgctgtgg
     4981 gacaatttgc ccgtgtggta caagaccaag tggctctcgg agaccttcgc cgcgcacgac
     5041 gcctgcctgg tcgccgacac ctacacctcg gcgtggagcg gacaggtcga gaagctccgc
     5101 gaggacgatt tcctcgaaag catggtggag ggctactcga ccatctacct caatgtctcg
     5161 gtggccgaga tggcgcgctc ggtgcttggc atgatcgaca agtacggcgc ggacggcgtg
     5221 gtgatgcact ccaatcgcag ttgcaagccg tactcgctgg ggcagtacga catccagcgc
     5281 atcattcaga aggagcgcga cgttcccgtt ctcatgatcg aggccgacat ggtggacgag
     5341 cgcgtcttca gcgagagcca gatcggcacg cgcatcgacg cattcatgga ggtggtccgt
     5401 tcgcggaaat ccggctgatc attttccgtc gtcggtaccg gagacgccgt accagcgaat
     5461 gagcgtattt tcgagcgtgt agtagtgggc gacgtagaag gtggagagcg cgcacaggat
     5521 gccgaagacc gcacacagca acacggaatc gtgctgcagg tacagtccca agaacagcac
     5581 atcgatgatg atgaccgcga acatcgttat caggtgcgcc aggcgttcgt gctgtagcat
     5641 ggcgattcgc gtggcgtgac gcgccggaaa tccgggatca ccccgcgagg cgtcgatgta
     5701 ggcgtcatgc gatctgataa gttctttcac gcttttcatg gccacacccc gatttttgtt
     5761 ccgcttcttc ttgacaggga gatcgttcaa tagtactata ctgtacgcgt gcagtcatac
     5821 atcaggcacg gaggtgcgat atgataccgg acaccatcga aaagatcgag aaaaaaatca
     5881 acggctcccg aacaattagc gacgagaaga agcgcgaact gctcaagctg gtcgatgagc
     5941 tcaagcgcga ggtgcttgcg gcctccgggg acgaccggga cggcgcgcag agcgtcgcga
     6001 atttcgcgaa cgcctccgcg cacgaggcgc tgcgggagat cgtcgatccg cgcatgttcg
     6061 agctcgcgcg aaacgggctc tccgagtcgg tccgtcggtt cgaggtcacg aaccccgggt
     6121 tggtgtcggt cgtaaacgcg ctctcgaccg tgctggccaa catgggtatc tgacgcgcgc
     6181 gatgtgacga tggacctcca gcagtatgtg tacgagcgaa ccgagggcct gcagaacggg
     6241 cagcggtcgc tctacggatt ttcccgcgag ggcagcttta agaaatgggt gctcgggatc
     6301 gagcggatca gtttcgccag cccgttcagt gaatggattt tcgtaacggt gccgacgacg
     6361 gaaagcggcg tgtcgccgga gcacgccgat gagcaaccgt acggctggaa cggccacatc
     6421 aacgactatg ccgccgagtc cgccgtctgg tatgcctttg atcttctcac cgaaacggaa
     6481 gcgcaggagt tcatggagaa ccatcggccg gaggtcattt tcgaattcgt ggagcgcggc
     6541 catccggagc ggctgcaggt acgctacgag aacgggcggt ggattgtcgt cgatgagatt
     6601 gaagagatcg ggggcatcgg ggcgcgctag gcgcggcgcg cataataccg cgccagcgga
     6661 atgcccgcga taaaaccgca caggtggctc tcccacgaca cgtgcggcga tgacggaatc
     6721 accccccaga cggccccgcc gtaaagaaaa aagattgcga tggacacgat gatcgatttg
     6781 agatcgcgtt tgaagacgcc gaagaaaaag agatagctca acagtccgaa gacgaggccg
     6841 ctcgcgccga tgtgaacggt gcccggccgg ccgatgagcc atgtcccgaa acccccgagg
     6901 atggcgagcg aaatcatgat ggtgagcgcg cgccccttct ccagcgcgat gaggacggtg
     6961 cccaggacga acaggctgaa tgagttcgcg atgaggtgcg cggcgtcctg gtgcaggaag
     7021 ggcgcggccg cgacgccgac aagcccggtg atggtgcggg gaacgatccc gaggtttttg
     7081 atgggaaacg cggcgctggc caggagaatg atccacagca ggagaacgaa tcccgccatg
     7141 aacttgaccg cgccgagaat tccctgccgt tcaatcatgc cgcactcacg ccccgtaaat
     7201 cgccgtcaat ccttgaacac catg
//
LOCUS       DAORHF010000189         7195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10143_length_7195_cov_4.254762, whole genome shotgun sequence.
ACCESSION   DAORHF010000189 DAORHF010000000
VERSION     DAORHF010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7195
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10143_length_7195_cov_4.254762"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="PKJ16_13870"
     CDS             <1..328
                     /locus_tag="PKJ16_13870"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV48127.1"
                     /translation="EFMDMHPERVQQLHENVEYFVNGLRKAGFSVVCHSAIIPLLVPE
                     QYNVLKLVNMLHREGVFVNGVMYPAVPKDRQRLRISMMATFTKEELDFAVSKILFVAK
                     KIGWMK"
     gene            339..1706
                     /locus_tag="PKJ16_13875"
     CDS             339..1706
                     /locus_tag="PKJ16_13875"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HNV48128.1"
                     /translation="MEPIAPHDRLLVLMTLNAFSTEEKREAISLAGSVTDWNEFWRVA
                     DDNRTAPLVQYNMEKLKIFSLVPKTVRDRFVARSAEIKNANEGRLAVAKKFLKKFNER
                     KIPVVILKGVNFAETIYKNPYYKRMNDIDILIKFADIDAIYEVYEEMGFFCAAELVGG
                     NPRKQEKFSHHAPSFFSRDLKCMIGTHWGLITPMAPYRIDYPAIWSRVRDFKFYNLKV
                     LTMAPEDNLHHLCVHLPYYKAGLKEIADIYNLVRHYGNEIDWSVFLREVAKAKTENPV
                     YHALSLANRLCPVPKFDEIIAAVKPKTSWYFRRDTARKIRDLAILVRMRSVHMARIEK
                     NFSDFNITKKALEKWKYYLLMWHGLLFPPIEDVIKMNAYRDPGLGRRLLGRVITPLKI
                     FRVFSRDLGAKIFILILIKCFVESIWYTLKAPFSRGTEIDYAAFARRIGCSVEDLQRL
                     KDNLE"
     gene            1703..4252
                     /locus_tag="PKJ16_13880"
     CDS             1703..4252
                     /locus_tag="PKJ16_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV48129.1"
                     /translation="MTFAIPLEGLTSRDLPLVGGKAMGCARLMQRRFTVPRGFAITTE
                     SFRFFLSQAVLSEKLAAIHQSLNRGDDESLTEAACAVLQSSIRNADLPGALADVIDRF
                     ASNLSAPLAVRSSATVEDAADFAFAGIFHSELRVSRETLADAIRDCWAHAVSYQVLLQ
                     ILRNNLNPAKTGVALLVQEMVAADFAGVMFTDDPTEARPGCALVSLTKGTGEGLMQGD
                     SAGDFIVYDRARRAIVDGEAPAGITPQALSGLFKSGMAIEKAFGTPMDIEWAYAGGTM
                     HYLQARPITTIENRHTRTIHWTRELTEERYPRPISPLGWSLLHNVLLENMQTLSRRFG
                     LVAKHPEEVAKTIRHYVYSNKKFFSVPGSIRPNPFKMIALLPRFAMQLPIILLFALPA
                     LISRNGFGLKWLLLSRFFGAFIFPHARDIRSKWERHLSSILREMDAYHDLDYSRMTLG
                     ELLDAHRRMDALACRYMEPDLAIYIVKIACSWIIERMGATIRGERYLSYLTDITSGIG
                     ENRTLHMNAEIEEMSDFLIAWPAIKQLLLDERYDDAAGAIERENPELYARFLRENGHV
                     TTNWDIMESTWGEEPVKIMQMLRGYLVTKRRHSFSDHRDERAGRYEAARDDTLLRLRR
                     TPWMTPFFLKVQRYLREFMSIDEEHHFYCSRLFKPLRMVFLEIGKRLADRGVIDSSDD
                     VFFLTIPEIRDVNGEGVHFSRRNLVTMRRSSFERSAAVRPPDDYINQTPLARERDTIP
                     EDGSLRGVGASSGIASGPARIVTTTEEAREFREGEILITVAPNPLWTSLFAIAGGLVA
                     STGSILSHGLVSAREYQLPAVIGIADVTSKISNGQRIQVDGDTGIVSFLER"
     gene            4215..4997
                     /locus_tag="PKJ16_13885"
     CDS             4215..4997
                     /locus_tag="PKJ16_13885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV48130.1"
                     /translation="METPGSFPSSSAEIDTLLFDLDGTLVDITWRGLELVFMLRAIRR
                     FRGAIAPWKFYFAFWDAVKTSKRHMTDRTNHQVLLETLHAHSTTTLEDITSRVHAYID
                     EDFAKLAGYFSPIPGARETILLARDLGYRVVVATNPSMPYQTVRLRLDRAGVGDIDYD
                     YITHSENSTRCKPSLEYYRELLGAIGARPAQCLMIGNDIEKDLPAREIGILTYMLDTP
                     HVHRQIKRLAAAQRLQGCGDFVRLQGWLRASGPRSTREAPSP"
     gene            4994..5644
                     /locus_tag="PKJ16_13890"
     CDS             4994..5644
                     /locus_tag="PKJ16_13890"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48131.1"
                     /translation="MKSLEGSMNGSNFICITGPDGSGKSTAIEGVYQRLTEEKKLRVA
                     KVSIWDMLLDPATRGKVGFKSKAELDQFFTILHPVARSLFLFHCIYQALHMASQKSAD
                     LYLIDSYWYKYYATEIAHGADQDALESITAIFPQPRLTFYLDVDTGETSRRKETISGY
                     ESGFSSGAKGSFVEFQKTAAASLEALRQRYGWITIPGAKSPVEVQEEIVSRISEVF"
     gene            5914..>7195
                     /locus_tag="PKJ16_13895"
     CDS             5914..>7195
                     /locus_tag="PKJ16_13895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48132.1"
                     /translation="MSQFKETSVTANFQNQAAKYGDRACVAYKKDGAYVDISWKKMNE
                     MVRQLGQYLMSIGVKKGDRVAVFANNRYEWWVSDLAILSIGAVNVPIYATNSPDEAHY
                     VLDHSESKVCLCGTESHLDRVLQVKKKLKKLKSLIVFETPKKKTVGVLTLDEAFKKGA
                     TYKDKKAFDKRLSAIKPNDLATIIYTSGTTGNPKGVMLSHNNFLSNVNQIMHDYREHL
                     SDQDVFLSFLPLSHSLERTAGYYMPIKQGAKVAFAEDVSTMQQNLTEVRPTIMISVPR
                     LYEKIHAGILAKLADAPAVKKTLATAAFKTAAKNVPGICEGKKADELGFAYKLFNKLI
                     YTKLKVALGLDRFKFAVSGGAPLAVTDAEFFIGMGIIIVEGFGLTETTPVTNANRFGM
                     IKPGSVGPAIHDTIVKISDEGEILIKGPQVMLGYY"
BASE COUNT         1561 a         2081 c         2030 g         1523 t
ORIGIN      
        1 ggagttcatg gacatgcatc cggagcgggt gcagcagctg catgaaaacg tcgagtattt
       61 cgtgaacggt cttcgcaagg ctggtttttc ggtggtgtgt cactccgcca tcatccccct
      121 gctggtcccg gaacagtata atgtgttaaa gctcgtcaat atgctccatc gggagggcgt
      181 cttcgtgaac ggcgtcatgt acccggccgt cccgaaggac cgccagcgct tgcgcatcag
      241 tatgatggcc accttcacga aggaggagct ggacttcgcc gtatcgaaga ttcttttcgt
      301 cgcgaaaaaa atcggctgga tgaagtaaag gagctcacat ggaaccgata gccccccacg
      361 accgcctgct cgtgctcatg accctgaacg ccttctcgac cgaagagaag cgcgaggcga
      421 tatcccttgc gggttccgtc accgactgga acgagttctg gcgcgtggcc gacgacaacc
      481 gcaccgcgcc gctggtccag tacaacatgg aaaagctgaa gatattcagc ctggtcccga
      541 agacggtgcg cgaccgcttc gtcgcgcgtt cggctgagat caagaatgcg aacgaggggc
      601 ggcttgccgt cgcaaaaaag tttctcaaga aattcaacga gcggaagatc ccggtcgtca
      661 tcctcaaggg cgtgaatttc gccgagacga tctacaagaa cccgtactac aagcggatga
      721 acgacatcga catccttatc aagttcgcgg acattgacgc gatctacgag gtctacgagg
      781 agatggggtt tttctgcgcg gcggagctgg tgggcggcaa cccgcgcaag caggagaagt
      841 tctcgcacca cgcgccgtcg ttcttcagcc gcgacctgaa atgcatgatc ggcacgcact
      901 gggggctcat cacccccatg gcgccgtacc ggatcgatta tccggccatc tggtcgcgcg
      961 ttcgagactt caaattctac aatctcaagg tcctcaccat ggcgcccgag gacaatcttc
     1021 atcatctgtg cgtgcacctg ccgtattaca aggccggcct caaggagatc gccgacatct
     1081 acaacctggt gcgccattac gggaatgaaa tcgactggtc ggtgttcctc cgcgaggtcg
     1141 cgaaggccaa aacggaaaac ccggtatacc acgcgctctc gcttgcgaac cgtctctgcc
     1201 cggtcccgaa gttcgacgag atcattgcgg ccgtgaaacc gaagacgtcg tggtacttcc
     1261 gacgcgatac ggcgcgcaag atccgcgacc tcgcgatact cgtcagaatg cgttcggtgc
     1321 acatggcgcg catcgagaaa aatttcagcg atttcaacat cacgaagaag gcgctcgaga
     1381 agtggaagta ctatctgctg atgtggcacg gccttctctt cccgccgatc gaggacgtca
     1441 tcaagatgaa cgcgtatcgc gatcccggcc tcggccgacg cctgctggga cgcgtcatca
     1501 ctccgctcaa gatatttcgg gtcttcagcc gtgacctcgg ggcgaagatt ttcattctga
     1561 tcctcatcaa gtgcttcgtg gagtcgatct ggtacacgct gaaggcccca ttttcacgcg
     1621 gcacggaaat cgactatgcg gcgttcgcgc gacggatcgg atgctcggtc gaagacctcc
     1681 agcggctcaa ggacaatctc gaatgacctt tgcgatcccg ctcgaagggc tgacgtcccg
     1741 cgatctgccg ctcgtcggcg ggaaggcgat gggatgcgcc cggctcatgc agcgcagatt
     1801 caccgttccg cgcggtttcg ccattaccac ggaatcgttt cgtttctttc tttcgcaggc
     1861 ggtcctctcg gaaaagcttg ctgcgataca tcaatcgctc aatcggggcg atgatgaatc
     1921 gctgaccgag gcggcctgtg cggttttgca atcatccatt cgcaatgccg atcttcccgg
     1981 cgcactcgct gacgtgatcg atcggttcgc ctcgaacctc agcgccccgc tcgccgtccg
     2041 ctcgtcggct acggtcgagg acgcggccga tttcgccttt gcgggaatct tccattccga
     2101 gctgcgcgtt tcgcgcgaaa cgctggccga cgcgatccgc gattgctggg cgcatgccgt
     2161 gagctatcag gtgctgttgc agattttgcg caataacctc aacccggcga aaacgggcgt
     2221 ggcgcttctg gtgcaggaaa tggttgcggc cgatttcgcg ggggtaatgt tcaccgacga
     2281 tccgaccgag gcccgacccg ggtgcgcgct cgtctcgctc acgaagggca ccggggaagg
     2341 gctcatgcag ggtgacagcg caggcgattt catcgtgtat gatcgcgctc gtcgcgccat
     2401 cgtcgatggt gaagcgcccg cagggatcac gccacaggcg ctgtccggac tttttaaaag
     2461 cgggatggcg attgaaaaag cctttggaac gccgatggac atcgagtggg cctatgccgg
     2521 cggcacgatg cactatctgc aggcgcgccc catcaccacg atcgagaatc ggcacacgcg
     2581 gaccatccac tggacccgtg agcttaccga ggagcgctat ccccggccca tctcgccgct
     2641 cggctggtcg ctgctgcaca acgtactcct cgaaaacatg caaacgcttt cgcgccgctt
     2701 cgggcttgtg gcgaaacatc ccgaggaggt cgccaaaacc atccgccatt acgtgtacag
     2761 caacaagaaa tttttctcgg taccaggatc gatccgcccg aatccgttca agatgatcgc
     2821 gctcctgccg cggttcgcga tgcaacttcc gatcatcctg cttttcgccc tgccggcgct
     2881 gatcagtcga aacgggttcg gcctcaaatg gcttctgctc tcacgcttct tcggcgcctt
     2941 catcttcccg cacgcgcgtg atattcgctc gaaatgggag cgacatctct cgtcgatcct
     3001 gcgcgagatg gacgcctatc acgacctcga ctactcgcgg atgacgctcg gcgagcttct
     3061 cgatgcgcat cgacgcatgg acgcgctcgc gtgccgctac atggaacccg acctcgccat
     3121 ttatattgtc aagatcgcct gttcgtggat catcgagcgc atgggcgcga cgatcagggg
     3181 cgagcgatat ctgtcgtatc tcaccgacat caccagcggc atcggcgaga accggacgct
     3241 gcacatgaac gccgagatcg aggagatgtc ggattttctg attgcctggc ccgccatcaa
     3301 gcaactcctg ctcgacgaac gatacgacga tgcggctggg gcgatcgagc gggaaaatcc
     3361 ggagctctac gcccggtttc tgcgggaaaa cggccatgtg accacgaatt gggacatcat
     3421 ggaatcgacc tggggggaag aacccgtcaa aatcatgcaa atgctgcgcg gctatttggt
     3481 caccaaacgc cggcacagtt ttagcgacca tcgcgacgaa cgcgcagggc gctatgaggc
     3541 ggcgcgggac gatactcttc tcaggctcag gcgcaccccc tggatgacgc cgtttttttt
     3601 gaaggtgcag cgctacctcc gcgagttcat gagcatcgac gaggagcacc atttctactg
     3661 ctcccggttg ttcaagccgc ttcgtatggt ttttcttgaa attggcaaac gccttgcaga
     3721 ccgtggcgtg attgattcat cggatgacgt gttcttcctg acgataccgg agattcgaga
     3781 tgtgaacggg gagggcgtgc atttttcccg gcgcaacctg gttacgatgc gtcgaagcag
     3841 cttcgagcga agcgctgccg tccgtccgcc cgatgattac atcaaccaga cgccattggc
     3901 ccgcgagcgc gacacgatac cagaggacgg atcattgcgc ggcgtcggtg cgagctcggg
     3961 gattgcatcg ggtccggcgc gcatcgtcac caccaccgag gaggcgcgcg aattccgcga
     4021 gggcgagatt ttaattaccg tcgcgccaaa cccgctttgg acgtcgttgt tcgcaatcgc
     4081 gggcggcctg gtcgcctcca cggggagcat cctgtcgcac ggactggtat cggcgcgcga
     4141 gtatcagttg ccggcggtca tcggcatcgc cgatgtcacg tcaaagattt caaacggtca
     4201 aaggatacag gtcgatggag acaccgggat cgtttccttc ctcgagcgct gagatcgaca
     4261 ccctcctgtt cgatctcgac gggacgctgg tggacatcac ctggcgcggt ctcgagcttg
     4321 tctttatgct gcgtgccatc cggcgcttcc ggggcgcgat cgcgccatgg aagttctatt
     4381 tcgccttttg ggatgcggtg aagaccagca agcgccacat gacggaccgg acgaaccatc
     4441 aggtgctcct tgaaacgctc cacgcccact cgactaccac gctcgaagat attacctcgc
     4501 gcgtacatgc ctatatcgac gaggatttcg cgaagcttgc cggatatttt tctccgatac
     4561 ccggcgcgcg ggaaacgatc ctgctggcga gagacctcgg ctatcgggtt gtcgtcgcga
     4621 cgaatcccag catgccatac cagaccgttc gcctgcgact cgatcgcgcg ggggtgggtg
     4681 atatcgatta cgattatatc acgcatagcg agaacagcac ccgctgcaaa cccagcctgg
     4741 agtattaccg cgaactcctg ggtgccatcg gcgctcgtcc cgcgcagtgt ttgatgattg
     4801 gaaacgacat cgagaaggac ctcccggcgc gggagatcgg cattctcacc tatatgctcg
     4861 atacgccgca tgtgcatcga cagatcaagc gcctggccgc cgcacagcgg ctccagggat
     4921 gtggagactt tgttcggctg cagggatggc tgcgcgcctc ggggcctcgc agcacacggg
     4981 aggcgccatc accatgaaaa gcctggaggg ttcgatgaat gggagtaact ttatttgcat
     5041 aaccggcccg gatggatcgg gtaaatcgac ggccatcgag ggagtgtatc aacgcctgac
     5101 cgaagagaag aagctgcgag tggcgaaggt ttccatttgg gacatgctcc ttgatccggc
     5161 gacgcgcggc aaggtcggat tcaagtcgaa ggccgaactt gatcagttct ttacgatact
     5221 gcacccggtt gcgcgatcat tgtttctttt ccattgcatc tatcaggcgc ttcacatggc
     5281 cagccagaaa tccgctgacc tgtacctgat cgattcatac tggtacaaat actatgcgac
     5341 cgagatcgca cacggcgccg atcaagatgc gctcgagtcg attaccgcaa ttttcccaca
     5401 accgcgcctg accttttacc ttgatgtaga tacgggagaa acatcccgta gaaaagagac
     5461 gatttcgggc tatgagtcgg gcttttcttc cggcgcaaag ggttcttttg tagagtttca
     5521 aaaaaccgcc gccgcttccc ttgaagccct ccgacagcgt tacggatgga ttacgatccc
     5581 cggcgccaag agtcccgttg aggtccagga agagatcgtt tcccggatct ccgaagtatt
     5641 ctgatgtcgg tttttccatt aaaaaataat accttttcaa gagaagttga ttgacatttt
     5701 cctctcgttt actgaaatac ggtaatcaat agaatgttgt aattgtgctg ctcccggttc
     5761 gccgatccac ttcacctgcg ccgtttgcat gaacgtaacg gatgtggtaa tatgtctcat
     5821 tgtgacagga acggcatcgg ggcgccgtgt ttcagcagga tttattcact ttaaattata
     5881 ctaattttta gaaccaaatt tttggaggta cgaatgtcac aattcaagga aacatcggtt
     5941 actgccaatt ttcagaacca ggcggccaaa tatggagacc gggcctgcgt tgcatataag
     6001 aaagatggcg cctatgtcga tatctcgtgg aaaaaaatga acgagatggt ccggcagctc
     6061 ggccagtacc ttatgtccat cggcgtcaag aagggcgacc gtgtggccgt attcgcgaac
     6121 aatcgctacg aatggtgggt ttctgacctg gcgatcttgt cgatcggcgc ggtcaacgtt
     6181 ccgatttatg cgaccaactc tcccgacgaa gcgcattatg tgctcgacca ctcggagtcg
     6241 aaggtctgcc tgtgcggaac cgagagccat ctcgatcgcg ttttgcaggt caaaaagaaa
     6301 ctgaaaaagc tcaaatcgct gatcgtcttc gagacgccca agaagaaaac cgtcggggtc
     6361 ctcacgctgg acgaggcatt taaaaagggt gcaacataca aggataaaaa agcgttcgac
     6421 aaacgcctga gtgcgattaa gccgaacgat ctggccacga tcatctatac gtccggcacc
     6481 acgggaaatc ccaagggcgt tatgctgtcc cataacaact tcctgtccaa cgtgaaccag
     6541 atcatgcacg actaccgtga gcatctgagc gaccaggatg ttttcctgtc gttcctgccg
     6601 ctttcacact cactcgagcg gaccgccggc tactacatgc ccattaagca gggcgccaag
     6661 gtggcatttg ccgaggatgt ttcgacgatg cagcaaaacc tcacggaggt tcgccccacg
     6721 atcatgatca gcgtgccgcg tctctacgag aagatccatg ccggcatcct cgccaagctg
     6781 gccgatgcgc ccgccgtgaa aaagacgctt gcgaccgcag ccttcaaaac tgccgctaaa
     6841 aacgttcccg gcatctgcga agggaaaaaa gcggatgaac ttgggttcgc atacaaactg
     6901 ttcaacaagc tgatttacac gaaactcaag gtcgcgcttg gccttgatcg cttcaagttc
     6961 gccgtatcgg gcggagcccc gcttgcggtg accgatgccg aattttttat cggcatgggg
     7021 atcatcatcg tcgaaggatt cggccttacg gaaaccaccc cggtgaccaa tgccaaccgc
     7081 ttcggcatga tcaagccggg ttcggtggga ccggcgatcc acgacactat cgtgaagatc
     7141 tccgacgagg gcgaaatcct catcaagggg ccccaggtga tgcttgggta ttaca
//
LOCUS       DAORHF010000190         7161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10215_length_7161_cov_4.882775, whole genome shotgun sequence.
ACCESSION   DAORHF010000190 DAORHF010000000
VERSION     DAORHF010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10215_length_7161_cov_4.882775"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="PKJ16_13900"
     CDS             complement(<1..2217)
                     /locus_tag="PKJ16_13900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48133.1"
                     /translation="MEEKNRRRKRILLRYNLAVLLGYAIPLLTMYVARWQDLISTQYA
                     AIHVVAVYVGIISLVFILLGVLKKDISDRFANIVYVGQFIAWLCIYFYAIIIINEIRT
                     IALLWGSIGLVFLLSQAGFRLALTITTMAFLSYVVGAFVAIRVLRQNGSFVNELLYAL
                     VFLFVGLFLSFTAGLFKKQRSELKRFQKKLNEEKEKALEAEAALRASEEKYRHFIDNA
                     NDLIFKADWKGSFLMANEAFASTLGYSREEIFTLNCMELVAPEYREQAASFYINQMKD
                     KLAESILEIPVVRKNGEVIWLEQNAKSIFAEDGRFMEFDTISRDITRRKRAEEALRAS
                     EEKYRTIVNTDIIGYFEVDLKGNFVLCNDAFANFLRYKPDEMVGVSYQKIMSEETALK
                     VFEVYNIVFSGKSKYSYGEFEVIRKDGTIGYGANMVMPIVDEHAAIVGFGVLGIDITD
                     RKMAEEALRESEEKYRTVLDTNKVGMFEVDLGGSITFCNEVVAKLMGYSMEEFVGINF
                     SKIMTEQSMKRVFVEYHKVFRKEVNTTIVKHEVIRKDGSVIFIDTTVSLLADGSGKPK
                     GFRAIAIDVTERMRAEEALRQSEERYRLVLDNIEDMVFICGLEGKFAYGNKATEKVTG
                     FTIDEFIGSNYLHYIVRDDRERELNSYIRQVTENIDTTYHEYRFRKKNGDTAWMSQLT
                     KMVKSPDGAIEFYGIARDITDQKRAEEELKAAKDAAERANLAKSKFLAGMSHELRTP"
     gene            complement(2240..>7161)
                     /locus_tag="PKJ16_13905"
     CDS             complement(2240..>7161)
                     /locus_tag="PKJ16_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48134.1"
                     /translation="SVEELPDRILVIINQVNAGLAKMVSSGDAHRVIELEIMAAAKAK
                     ASSAYRSALNYLQTGISLLEDTAWKTRYELALRLYTEAADAAHLAAQFDDMNRYHELV
                     VKNARSQYDTLKVYDIRINYLLGQGKARDAVAVGSAVLASFGMRIPVKPGNGFIAWNY
                     LMTRMKMIGTHAAEIDTRPSATNPRAIAIMRIINSLAAASYIGSIGFFLSAIFKTNQL
                     MARYGNTDTSPLYYLGYAIVLATTGKITEGYALGESGCALANAMGDAVQMFRTAFQMN
                     VWIRHWKEPLRDSVDPLMDLYRRGVELGNFEYASYALCVRGYHLFHAGRNLTTISADV
                     AKSIAGISKLNHEAILNLTRSGRQIVLNFMGECEDPCRLKGDSYDEDAVTLTDTDHFY
                     RMILLYHFYRFDDALEEARHVERRVRSFTCLAHVPVIVFYSSLILLALIETRRKKDRK
                     RFLKRIRKNQAQMRKWAAHAPMNYSHKLLLVEAELQRVRGNFGAAMKRYEESIAAAHQ
                     NGYLHEEALASELASHLYEANGFSVIAATYMMRAHRCYTTWGGEGKTSFLEKRFPGRI
                     RTYDPLAHATSVLPTTTSGNISELLDVASILKSSQVIASETELALLLEKTLAIALENA
                     GAQRGLFIMEHDGDSDARRLFIEAEASLEGAVRILEPVPLEEYRKAPRSLVHYVDKTG
                     EDLILNDVAMDERFGADKYISQQQVKSVLCMAIRSKGAVLGIMYLESSLVAGAFTPRR
                     LHIMRILTSQAAISIENARLLMRLTDLNVNLEQKVMDRTAELKSTLEAMEFVNDNLLA
                     TTSELEDAQVKLRESEEKYRSLLDSGIIGYYELDLRGNFTYCNKSFLAFTGFSEDEII
                     GMNFRQVIVRQDVRKMLREFAGVYRGTNEVGIISSRSMRKDGQIIYGDNYVRLRKNAA
                     GEIVGFSSVAINVSDRTQAEEALRESEEKYRSILDAGIIGYYELDLRGCFTFCNKSFL
                     EFLGYAAEELIGMNFREVTLPEDHGRLLGDFARVYQGIVQVGIIAMRAISKTGELVYG
                     ESYVSTRRNTAGEITGFRSVGINTTERRNAEEALLISQEKYRHFIENANEVIYKSDWR
                     GNFIFANEAFTRFLGYSNDEILRMNYLDVMVPERREDEFLFYSEQLKGRVAESSRELP
                     VRAKDGRVLWIEQNVKSIFGEDGRIVEFDAIAHDITDRKMAEEALRESEERYRLVLEN
                     VDDGIFICGVDGRFKYANKAIRRVSGYDLDEVVGSSFLTFIHAEDRERELASYVTQVA
                     ENIDVTYHEYRFIHKNGIVHWIAQTSRMVRDKNGEIEFYAIGRDITDQKRAEEELKAA
                     KDAAERANLAKSKFLAGMSHELRTPLNAINGIVELLRFGSYEKDEEIGEEIRNVIRLL
                     EERAVDDMRTMTDGIIAELGSILVHLEADGNLKQYFFSKMEKELSSDDFQDDEVIGAI
                     RRISGLIDEEEREIFHSYKRIKDAGDYLLGLIDTVLNLAKIETGKIEVAMARTNVREM
                     VESVVHDAKSYARAKGKEGVLVIEHAVDDAVPAAVSLDNQKTRQVLLNFLSNAIKFTA
                     EGGVRLAVETRDGAVVFSVSDTGIGIKPEERDRLFQEFGRTEETQFIEGSGLGLVISK
                     RLIELQGGAVGFDSEYGAGSVFWFTLPSE"
BASE COUNT         1461 a         2280 c         1807 g         1613 t
ORIGIN      
        1 cggcgtccgc agctcgtggc tcatccccgc caggaacttg ctcttggcga ggttcgcccg
       61 ctcggcggcg tccttggccg ccttcagctc ctcctcggcc cgcttctggt ccgtgatgtc
      121 gcgggcgatc ccgtaaaatt caatcgcgcc atccgggctt ttcaccatct tcgtcaattg
      181 cgacatccag gcggtatcac cgtttttctt cctgaagcgg tattcgtgat atgtggtatc
      241 gatgttttcg gtcacctgcc ggatatacga attgagttcg cgttcgcggt catccctgac
      301 gatataatgc agataattcg atccaatgaa ttcatcaatc gtgaacccgg tgaccttttc
      361 cgtcgctttg ttgccatacg cgaatttgcc ctcaagaccg cagatgaaga ccatgtcttc
      421 gatattgtcg aggaccaggc gataccgctc ctcgctctgc cgcagcgcct cctcggcgcg
      481 catccgttcg gtcacgtcga tggcgattgc gcggaagccc ttgggctttc ccgacccgtc
      541 cgccaggagc gatacggtcg tatcaataaa aatcacgctc ccgtcttttc tgataacctc
      601 gtgcttgacg attgtggtgt ttacctcttt ccggaaaacc ttgtggtatt caacaaacac
      661 ccgtttcatc gattgttccg tcattatctt cgaaaaattg atgccgacaa actcctccat
      721 cgaatagccc atcaattttg cgacaacctc gttgcagaat gtaatgcttc cccccaggtc
      781 gacctcgaac atcccgacct tgttggtgtc cagcacggtc cggtatttct cctcgctctc
      841 ccgcagcgcc tcctcggcca tcttccggtc ggtgatgtcg ataccaagca cgccaaatcc
      901 cacgatggcg gcatgttcat cgacaatcgg catgaccata ttcgccccat agccgatggt
      961 gccgtccttt cgaatgacct cgaactctcc gtacgaatac ttgctcttcc ccgagaatac
     1021 gatgttatac acttcaaaca ccttcagggc ggtttcctcg gacattatct tctggtagct
     1081 cacgcccacc atctcgtccg gcttgtatcg caggaagttc gcgaacgcgt cgttgcataa
     1141 cacaaaattg cccttcaggt cgacctcgaa ataaccgatg atgtcggtgt tgacgattgt
     1201 ccggtacttc tcttcgcttg cgcgcagagc ctcctcagcc ctcttccgcc tggtaatatc
     1261 acgggagatt gtgtcgaatt ccatgaaacg tccgtcttct gcgaatattg atttagcgtt
     1321 ctgctccagc cagatgacct cgccgttttt tctgaccacg ggaatctcga gaatcgattc
     1381 cgcgagtttg tctttcatct ggttaatata gaacgatgcc gcttgttccc ggtattcagg
     1441 ggccaccagc tccatacagt tcaacgtgaa gatctcttcg cgggaataac cgagggtact
     1501 ggcgaaggcc tcattggcca tcaggaaact ccccttccag tcggccttga agattaaatc
     1561 attggcatta tcgataaagt gccggtactt ctcctcgctg gcgcgaaggg ccgcttcggc
     1621 ctcaagggct ttttcttttt cttcattcag ctttttctga aatctcttca gttcgctccg
     1681 ttgcttttta aacaatcctg ccgtaaagga aagaaacaac ccgacaaata aaaacaccag
     1741 cgcatacagc aactcgttaa cgaaggaacc gttctgccgt aaaacacgaa tggcgacaaa
     1801 agccccgaca acatatgaga gaaaggccat cgtggtgatc gtcagggcca acctgaaacc
     1861 ggcctgggaa agcagaaaaa ccagtccaat agatccccag agaagggcga ttgtcctgat
     1921 ctcattaata attataatcg cgtagaaata aatgcacaac caggcaatga actggccgac
     1981 atacacaatg ttggcgaatc ggtccgagat gtcctttttg agcacgccaa gcaggatgaa
     2041 aaccagtgaa attataccga catagaccgc tacaacatgt atggcggcat actgcgtgct
     2101 gattaaatcc tgccaccgcg ccacatacat ggtgagcaac ggtatggcat accccagcaa
     2161 cacggccagg ttatagcgta atagtattcg ttttcgcctt ctgttctttt cttccatgtc
     2221 gccaatcacc acatgccagt tactccgacg gcagcgtgaa ccagaacacg ctgcccgcgc
     2281 cgtattcgga atcgaagcca accgcgcccc cctgaagctc gatcagccgc ttcgagatga
     2341 cgaggcccag cccgcttccc tcgatgaatt gcgtttcctc ggtcctgccg aactcctgga
     2401 acagtcggtc gcgctcctcg ggcttgatgc cgatccccgt gtcggacact gaaaaaacaa
     2461 ccgccccgtc cctcgtctcg acggcgagac gcacgccccc ctccgccgtg aacttgatcg
     2521 cgttcgaaag aaaattcaag agcacctgcc gcgtcttctg attgtcaagc gacaccgccg
     2581 ccggaacggc gtcatcgacg gcatgctcga tgaccagaac gccctccttc cccttggcgc
     2641 gggcatagct cttcgcatca tggacaaccg attccaccat ctcccggacg ttcgtccggg
     2701 ccatcgcgac ctcgatcttc ccggtctcga tcttggcgag gttcaggacc gtgtcgatca
     2761 gccccaacag atagtccccc gcgtccttga tccgcttgta cgagtggaaa atctcgcgct
     2821 cctcctcgtc gatgagcccg gatatgcgtc gaatggcgcc gatcacttcg tcgtcctgga
     2881 aatcgtcgct cgacagttcc ttctccatct tcgaaaaaaa atactgtttc agattgccat
     2941 ccgcttcgag atggaccagg atcgacccca gctccgcgat gatcccgtcc gtcatggtcc
     3001 tcatatcatc aacggcccgc tcttccagca gccggatgac attccgtatc tcctcgccga
     3061 tctcctcgtc cttctcatac gaaccgaagc gcagcagctc gacgatcccg ttgatcgcgt
     3121 tgagcggcgt ccgcagctcg tggctcatcc ccgccaggaa cttgctcttg gcgaggttcg
     3181 cccgctcggc ggcgtccttg gccgccttca gctcctcctc ggcccgcttc tgatcggtga
     3241 tgtcgcgtcc gattgcgtag aattcaattt caccattctt gtcgcgcacc atgcgtgacg
     3301 tctgggcaat ccagtggacg atcccattct tgtggatgaa ccgatactcg tgataggtca
     3361 cgtcaatgtt ttccgcgacc tgggtaacat aggaggcgag ctcccgctct cggtcttcag
     3421 cgtgaatgaa tgtgagaaag ctgctgccga cgacctcatc aagatcgtac cccgacacgc
     3481 gccggatcgc cttgttggcg tatttgaacc tcccgtccac cccgcatatg aagattccgt
     3541 catcgacgtt ctccaggaca agccgatacc gctcctcgct ctcccgcagc gcctcctcgg
     3601 ccatcttgcg gtcggtgatg tcgtgggcga tggcgtcgaa ctcgacgatc cgcccgtcct
     3661 caccgaatat cgacttaaca ttttgctcga tccagagcac ccggccatcc ttggcacgca
     3721 ccggcagctc gcgggacgac tccgccaccc tgcccttcaa ctgctcgctg tagaagagaa
     3781 actcatcctc gcgccgttca ggcaccatca cgtcaagata attcatccga aggatttcat
     3841 cgttggaata gcccaggaac cgggtgaagg cctcgttcgc gaaaataaaa tttccgcgcc
     3901 aatccgattt ataaatcacc tcgttcgcat tctcgatgaa atgacgatac ttctcctggc
     3961 ttatcagcag ggcctcttcc gcatttctgc gctccgtcgt attgatgccc acgctcctga
     4021 acccggtgat ctccccggcg gtgttcctcc tggtgctcac atagctctcc ccgtacacca
     4081 gctctccggt tttggagatg gcacgcatgg cgataattcc aacctgaacg atcccctggt
     4141 acacgcgggc gaaatccccc aacaacctcc cgtgatcctc ggggagcgtg acctcgcgga
     4201 aattcattcc aatgagctcc tccgccgcat acccaagaaa ttcgaggaag ctcttgttgc
     4261 agaacgtaaa gcacccgcga agatccagct cgtaataccc gatgatcccg gcgtcgagaa
     4321 tggagcggta cttctcctcg ctctcacgga gcgcctcctc ggcctgcgtc cggtcgctga
     4381 cgttgatggc cacgctgctg aacccgacaa tctcccccgc ggcgtttttc ctgagcctga
     4441 cgtagttgtc gccgtaaatg atctgcccgt ccttgcgcat tgaacgcgag ctgatgatcc
     4501 cgacctcgtt cgtgccccga tagacgccgg caaactcccg cagcattttc cggacgtcct
     4561 gcctcacgat cacctgccgg aagttcatcc cgataatctc atcctcggaa aagcccgtga
     4621 acgccaggaa actcttgttg caataggtaa agttcccccg gaggtcgagc tcataatagc
     4681 cgatgatgcc cgaatccagc agggagcggt acttctcctc ggattcgcgc aatttgacct
     4741 gcgcatcttc aagctcggag gtggtcgcca gcaggttgtc gttgacgaat tccatcgcct
     4801 cgagggtcga tttgagctcg gcggtccgat ccataacttt ttgctcgagg ttcacattga
     4861 ggtcggtgag tcgcatcagc agccgggcgt tttcgatgga gatcgccgcc tgcgacgtca
     4921 ggatccgcat aatatgaaga cgccgcggcg taaacgcgcc cgcaaccaga ctgctctcga
     4981 gatacatgat tccgagaacc gcccccttgg aacggatggc catgcacagg accgacttca
     5041 cctgctgctg ggagatgtat ttatccgcac cgaaccgttc gtccatggcc acatcgttca
     5101 ggatgaggtc ctcccccgtc ttgtccacat agtggacgag ggaccgcggc gccttgcgat
     5161 actcctccag cggaaccggc tcgaggatcc gcacggcacc ctcaaggctg gcctcagcct
     5221 cgataaaaag ccgtcgggcg tcactatcgc cgtcatgttc catgatgaac aggccgcgct
     5281 gtgcccctgc gttctccagc gcgatggcga gcgtcttctc gagcagaagc gcgagttccg
     5341 tctcgctcgc gatcacctgt gaggacttca ggatcgaggc gacatcgagc agctccgaga
     5401 tgtttcccga cgtcgtggtc gggagtacgc tcgtcgcgtg ggccagcgga tcgtacgtgc
     5461 ggatgcgccc ggggaagcgc ttctcgagaa aggacgtctt cccctccccg ccccaggtcg
     5521 tgtagcaccg gtgcgcccgc atcatgtacg tcgccgcgat gaccgagaac ccattggcct
     5581 cgtacaggtg tgacgccagc tcagaggcca gcgcctcctc gtgcaggtag ccgttctgat
     5641 gcgccgcggc gattgactcc tcgtagcgct tcatcgcggc gccgaagttc ccccgcacgc
     5701 gttggagctc cgcctcgacg aggagcagct tgtgggaata gttcatcggc gcatgcgccg
     5761 cccatttccg catctgcgcc tggttcttcc ggatccgctt caggaaccgc ttccggtcct
     5821 tcttccggcg cgtctcgatc agggcgagca ggatgagcga actgtaaaaa acgatgaccg
     5881 ggacatgcgc caggcaggtg aacgaccgca cgcgccgctc gacatgcctc gcttcctcga
     5941 gagcgtcatc gaaccgataa aaatggtaca gcaggatcat ccggtagaaa tggtccgtat
     6001 ccgtcaacgt gacggcatcc tcatcatagc tgtcgccctt gagccggcag ggatcttcgc
     6061 attcacccat gaagttcaac acgatctggc ggcccgacct ggtcaggttc aggatcgcct
     6121 catggttcag cttcgagatg cccgcaatgc tcttcgcgac atccgcggag atcgtcgtga
     6181 ggtttcgtcc cgcatgaaac agatgatacc cgcgcacgca caacgcatag gacgcatact
     6241 cgaaattccc caactccact ccccgacggt acaggtccat caacggatca acgctgtccc
     6301 gaagaggctc cttccagtgg cggatccaca cgttcatctg gaacgccgtt cgaaacatct
     6361 ggaccgcatc ccccatggcg tttgccagcg cacacccgga ttcacccaat gcgtatcctt
     6421 ccgtgatctt tcccgtggtc gcgagaacga tggcatagcc cagataatac agcggcgacg
     6481 tatccgtgtt tccatatcgc gccatgagct gattcgtttt aaaaatcgcg ctcaagaaaa
     6541 aaccgatgct cccgatgtag gacgcggccg cgagagaatt gatgatacgc atgatcgcga
     6601 tggcgcgggg gttcgtcgcg ctcggacgcg tgtcgatttc cgcggcatgt gtcccgatca
     6661 ttttcatccg cgtcatcagg tagttccacg cgatgaaccc gttgcccggc ttgacgggta
     6721 tgcgcatccc gaacgacgcc agcacggccg atcccaccgc gaccgcatcc cgcgccttcc
     6781 cctgtcccag gagatagttg atgcggatgt cgtacacctt cagggtgtcg tactgagaac
     6841 gcgcgttttt caccaccagt tcgtggtagc ggttcatgtc atcgaactgc gcggccaggt
     6901 gcgccgcatc ggccgcctcc gtatacaacc ggagggccag ttcgtaccgg gttttccagg
     6961 cggtatcctc gagaagcgag atgcccgtct gcaaatagtt gagcgccgat cgatacgccg
     7021 atgacgcttt cgccttcgcg gcggccatga tctcgagctc gatgacgcgg tgcgcatccc
     7081 cgctcgaaac catcttcgcc aggcccgcgt tcacctggtt gatgatcacc aggatccgat
     7141 cgggcagctc ctccacggag g
//
LOCUS       DAORHF010000191         7132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10285_length_7132_cov_4.873958, whole genome shotgun sequence.
ACCESSION   DAORHF010000191 DAORHF010000000
VERSION     DAORHF010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7132
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10285_length_7132_cov_4.873958"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="PKJ16_13910"
     CDS             <1..623
                     /locus_tag="PKJ16_13910"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV48135.1"
                     /translation="KTWLATLEKAESVDLLGEEKVTVKEKRGELTYAVQKVKLADDKV
                     GYIDNKHLADKPIVFSEDTKALDRPTTGSRVHATLEKGSIAFITGEKGNWVQVYAGEV
                     KPKLWVVDKWVNGGYTADEKVIQDAKDYEVILSLLREKKPEKEKDAKEKLEQLSKTSS
                     FFAELAGKKLAELSAPKDEDQPKEGVTKDLGGDLSKEAPGAMDSGK"
     gene            complement(686..1702)
                     /gene="fliG"
                     /locus_tag="PKJ16_13915"
     CDS             complement(686..1702)
                     /gene="fliG"
                     /locus_tag="PKJ16_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HNV48136.1"
                     /translation="MKSITTMTGIEKAAALLISLGPDVASEILRHLDGESIRRISEQI
                     AMIDSITSGDKEELIGEFLLDLKKRRGSLYGGENVAKEILVGALGDEKAHDVLSKLNR
                     QDLERGFDFLKDIETDVLVSFLQNEHPQTVAVTLAYLPPAKSAEILKLMDSGVAREVA
                     KRLAKMEKTSPEAVLEIARVIRKRYEESKAAGRQHEVVGGVDTLVSIMNHMSGDQEKG
                     LMEFFDSAMPSVARDIREKLFVFENVLNLTNQEIRVLIDEINDDELIARALKGAGDEV
                     RFKLLRNMSQNRASDIIKDMERMGPTRLSDIQSARDEIVGIMRALNDNGVIDLRKQMD
                     QYVE"
     gene            1875..2534
                     /locus_tag="PKJ16_13920"
     CDS             1875..2534
                     /locus_tag="PKJ16_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48137.1"
                     /translation="MPDSTIRNEAYQADILHAVEADKEIVFVLYSILDDTEEKIKYAL
                     AQILDRRARDDLFTPLYSCTKELIANAVKANAKRLLIDEGVITDPDDPIEVVKMIRSV
                     LNEKSLLEYGIKAKEHRLSTRVYFKAEPERFVIEVINNVPLDGKDLKRILERIEKSSK
                     YDSIAEFYMENPDPIAEGMGLGLSMVVVLIKSIDIDWRNFTVSTNGKDKTYARITVPL
                     A"
     gene            complement(2547..3149)
                     /locus_tag="PKJ16_13925"
     CDS             complement(2547..3149)
                     /locus_tag="PKJ16_13925"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48138.1"
                     /translation="MAKKNLEREERRKIQIVQAAYEVIATKGYNNFTIEDIANQAGLS
                     KGGVLHYFKTKEDILIHLLEQIYMIIEKTIRKRAEKYQSPERKIKAIIIGYVTIAKKQ
                     PAFYRVMADFWAQITINERIRDINTKIFEKMSNEFKKVIDMGKEQALFGKVDSSGIAY
                     AITAMLINTAMQWTFNSAIYNIDHVGRQCIRMVMSYLTRP"
     gene            complement(3278..4216)
                     /locus_tag="PKJ16_13930"
     CDS             complement(3278..4216)
                     /locus_tag="PKJ16_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV48139.1"
                     /translation="MKYLFGPVNSRRLGRSLGVDLVPIKTCSLNCIYCECGETTNCTD
                     AIAEYVPTAEVVDELRECLQACPLLDVITFSGFGEPTLHSGLGEIVAFLRREFPEYRV
                     ALLTNATRFDDPDVRRAVLAIDVIIPSLDAAGEDAFRKIARPAPGITAKRIIRGLVEL
                     RREYRGEMILELFIVSGINDTEDELKKLKEAIAMIGPDRVHLNTLDRPGAVEWIQPAP
                     MDRLRFVADFLAPYPVEIVGKPAHAGPVRPAGGDVANTIVATVARRPSTLEDLSSALG
                     LPAADVNRTVTRLMNDGVLEKRQSGRGNFYCLRERT"
     gene            complement(4225..4620)
                     /locus_tag="PKJ16_13935"
     CDS             complement(4225..4620)
                     /locus_tag="PKJ16_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV48140.1"
                     /translation="MAGRVLQYTNTERIIESGKTEQRMFIILEGEVEISLTDGKSRFA
                     VATLKKGDFFGEISLFTNTPRSANAHAVGNVQLAYIDNLAQLKAFLLKNPSFAAKMVT
                     ILAQRLAKTDEILIGKISELNRLQITGEV"
     gene            4779..6245
                     /locus_tag="PKJ16_13940"
     CDS             4779..6245
                     /locus_tag="PKJ16_13940"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HNV48141.1"
                     /translation="MERFFHPRSIVLFGASENPGKGGNQVLQNFRQYMARNGYPDLFI
                     VHPRASCINGVACYRSLDELPRNANGDTRLDLAVIVLPVPEVMAAVRACVAREVRGIL
                     IESGNLSNDADEAARFAAELRALIRGRDIRIVGPNSIGFNVPDLRYCTPIKYYSQFLE
                     SRERNVSIVGQSGLFVTGFVEWFFDAAGHGQPFGVSNIAAIGNKLDVNECDLLEYYLA
                     DAKTHAVGMYLEDIRDGARFADILRDNRAGRQKPIVLLKAGKSEKGKQAVSSHTGSLA
                     GSYAVVEAIAKQYGMMLVETYEELFEMLSVVCQFPRMRGGRVGVISVSGSGCVLSSDF
                     AEKYGIELPELDGATVARLRPLFPDWAPIHNPIDTWASVEKVGPEESFSRILDIFIES
                     GRFDCILMMTISSAFAAFDWQHIGAVQRQHPGIPIIAHFFGGQSINENMSFAMKHSIP
                     VIPNFEHIFKLLGILRDYSRRMDRAGKFRDGASGSRGG"
     gene            complement(6225..>7132)
                     /locus_tag="PKJ16_13945"
     CDS             complement(6225..>7132)
                     /locus_tag="PKJ16_13945"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HNV48142.1"
                     /translation="YRGREEWAALIAAGKVVVNGSASDADRPLAKGDLVAYTILLEEP
                     PVDGNIAILHDEESFLVADKPGNLPAHADGRFIKNTFIWMIRERYAAQGFAGKLNLVH
                     RLDRETSGVMLVSKIESAHRGLMRQFGAGTVEKEYRAIVRGVVAADAFEVGGYIVPDR
                     ASGISIKKRVVDDEEPGAKRAVTRFEVIERLERFTLLRCVPLTGRTNQIRVHLAHAGY
                     PIAGDKLYGRTDEEFLAYVHAVKAGEYDPRPWLEAPRHLLHAARLSFDHPVTRERLCF
                     EAPVPEDMRRFIFEAGSSASSAARP"
BASE COUNT         1340 a         2285 c         2185 g         1322 t
ORIGIN      
        1 tcaagacctg gctcgccacg ctggagaagg ccgagagcgt cgacctcctg ggagaggaga
       61 aggtcaccgt taaggagaag cgcggggagc tcacctatgc ggtccagaag gtgaaactgg
      121 ccgacgacaa ggtcggctac atcgacaaca agcacctggc cgacaagccg atcgttttct
      181 ccgaggacac caaggcgctc gaccggccca cgaccggcag ccgcgtccac gcgacactgg
      241 agaaggggtc gatcgccttc attaccggcg aaaagggcaa ctgggtccag gtgtacgcgg
      301 gcgaggtgaa gcccaagctc tgggtggttg acaaatgggt gaacggcggc tatacggccg
      361 acgaaaaggt gatacaggac gcgaaagact acgaggtgat cctgagcctg ctgcgcgaga
      421 agaaaccgga gaaggaaaag gacgcaaagg aaaagctgga gcagctctcg aagaccagca
      481 gctttttcgc ggaactcgcc ggcaagaagc ttgccgagct ctcggccccc aaggacgagg
      541 accagccgaa ggaaggcgtc acgaaggacc tcggcggcga cctgtccaag gaagccccgg
      601 gcgcgatgga cagcggaaag taaccgacga aaaaaaacga aacgccagcg gcggggaggg
      661 agacaccctc cccgctttcg taccctcact ccacgtactg gtccatctgc ttgcgaagat
      721 cgatcacccc gttgtcgttg agggcgcgca tgatcccgac gatctcgtcg cgcgcgctct
      781 ggatgtccga gaggcgggtg gggcccatgc gctccatgtc cttgatgatg tcgctcgcgc
      841 gattctggct catattgcgc agcagcttga agcggacctc gtcgccggcc cccttgagcg
      901 cgcgggcgat gagctcgtcg tcgttgatct cgtcgatgag gacgcggatc tcctggttgg
      961 tgagattgag gacattttcg aagacgaaca gcttctcgcg gatgtcgcgc gcgaccgacg
     1021 gcatggccga atcgaaaaac tccatgagcc ccttctcctg gtcgccgctc atgtgattca
     1081 tgatcgacac cagggtatcg acgccgccga cgacctcgtg ctggcgtccg gccgccttcg
     1141 actcctcata gcgcttgcgg atcacccgcg cgatctccag aacagcctcg ggcgacgtct
     1201 tttccatctt ggccaggcgc ttggccacct ctcgcgcgac cccggaatcc atgagcttca
     1261 gaatctcggc cgacttcgcg ggcggcagat aggcgagcgt gaccgcgacc gtttgcgggt
     1321 gctcgttctg caaaaacgaa acgagcacgt cggtctcgat atccttgagg aaatcgaacc
     1381 cgcgctcgag gtcctgtcga ttgagcttgg agagcacgtc atgcgccttc tcgtcaccca
     1441 acgcccccac gaggatttcc ttggcgacat tttccccgcc gtacaggctc ccgcgtcgtt
     1501 ttttcaggtc caggaggaac tcgccgatga gctcctcctt gtccccggag gtgatcgagt
     1561 cgatcatcgc gatctgttcg ctgatacgcc ggatgctctc accgtcgaga tggcgcagga
     1621 tctcggaggc gacatcgggc cccagggaga taagcagggc cgccgccttc tcgatgccgg
     1681 tcatggtggt gatggatttc atgtgggcac ccctttgcga acgtatgcgc cagggtgcca
     1741 cggaaacgca ggtccggcaa caaaataatt aatatttgcc cgccggggtg ccgaagtcat
     1801 aagagagcgg attgttcttg cgcgctcacg gccccgtgat agccgtgtac gatacaccaa
     1861 aggcaggccc ccggatgccc gattcgacca tacgcaacga agcctatcag gccgacatac
     1921 tccacgccgt cgaggcggac aaagagatcg tcttcgtcct ctactccatc ctggacgaca
     1981 ccgaggagaa aatcaagtac gccctcgcgc agatcctcga ccgccgcgcc cgggacgacc
     2041 tgttcacgcc gttgtactcc tgcaccaagg agctcatcgc caacgccgtc aaggccaacg
     2101 cgaagcgcct gctcatcgac gagggcgtca tcaccgatcc cgacgacccg atcgaggtgg
     2161 tcaagatgat ccgctccgtc ctcaacgaga agtcgctcct cgagtatggc atcaaggcga
     2221 aggagcaccg gctctccacg cgggtgtact tcaaggccga gcccgagcgc ttcgtcatcg
     2281 aggtcatcaa caacgtaccg ctggacggca aagacctcaa gcgcatcctg gagcgcatcg
     2341 agaagtcatc gaagtacgac agcatcgctg agttttacat ggaaaacccg gaccccatcg
     2401 ccgaggggat ggggctggga ctgtcgatgg tggtcgtgct catcaagagc atcgacatcg
     2461 actggcggaa cttcacggtt tcaaccaacg ggaaggacaa gacctacgcg cgcatcaccg
     2521 tcccgctcgc ctgacccgca cccctttcac gggcgcgtca gatacgacat caccatccgg
     2581 atgcactgcc gcccgacatg gtcgatattg tatatggcgc tgttgaaggt ccactgcatg
     2641 gcggtgttga tgagcatcgc ggtgatcgcg taggcgatcc ccgaggaatc gaccttgccg
     2701 aagagggcct gctccttccc catgtcgatc accttcttga attcgttgct catcttctcg
     2761 aagatcttcg tgttgatgtc gcggatgcgc tcgttgatgg tgatctgcgc ccagaagtcg
     2821 gccatcacgc ggtaaaacgc gggctgcttc ttcgcgatgg tcacataccc gatgatgatg
     2881 gccttgatct tccgctccgg gctctggtac ttctccgccc gcttccggat cgtcttctcg
     2941 atgatcatgt agatctgctc gagcagatgg atgaggatgt cttccttggt tttaaaatag
     3001 tgcagcaccc cgcccttcga caggccggcc tggttcgcga tgtcctcgat ggtgaagttg
     3061 ttgtagccct tcgtcgcgat gacctcgtat gcggcctgaa cgatctggat cttgcggcgc
     3121 tcctcgcgct caaggttttt cttcgccatc tgccattcct tgtgtgtgtg gtgattccgc
     3181 tacgcgcgtg cggccggcac gctgcgcctt tggtaggtac aggagtcttt tttgtcaagg
     3241 aaattaccgc catcgatcgg cggcggcatg attcgcgtca cgtccgctcg cgcaggcagt
     3301 agaagttccc gcgacccgac tggcgttttt ccagcacgcc gtcgttcatg aggcgcgtaa
     3361 cggtgcggtt cacgtctgcg gccgggagcc ccagcgcgct cgagaggtcc tcgagggtcg
     3421 aggggcgacg ggcgacggtt gcgacgatgg tgttcgcgac atcgcctccc gcgggccgta
     3481 cgggaccggc gtgggccggc ttgccgacga tctcgaccgg gtacggcgcg agaaagtcgg
     3541 ccacgaagcg gagccggtcc atcggggccg gctggatcca ttcgaccgcg ccgggccggt
     3601 cgagcgtatt gagatgcacg cggtccgggc cgatcatcgc aatcgcctct ttgagttttt
     3661 taagctcgtc ctcggtgtcg ttgatgcccg acacgatgaa cagttcgaga atcatctccc
     3721 cgcggtattc gcgccgaagc tcgacgaggc cgcggatgat ccgcttcgcg gtgatcccgg
     3781 gcgccgggcg cgcaatcttc cggaaggcgt cctcgccggc cgcatcgagg gacgggatga
     3841 tgacgtcgat cgcgagcacg gcgcgccgca cgtcgggatc gtcgaagcgc gtcgcgttgg
     3901 tgagcagcgc cacgcggtac tcaggaaact cgcgccgcag gaaggcgacg atctcgccca
     3961 gaccgctgtg gagcgtgggc tcgccgaacc ccgagaaggt gatcacgtcg aggagcggac
     4021 acgcctgcag gcattcacgc agctcgtcga ccacctccgc ggtgggcacg tattccgcga
     4081 tggcgtcggt gcagttcgtg gtttctccgc actcgcaata gatgcagttg agcgagcagg
     4141 tcttgatcgg gaccaggtcc acgcccaacg agcgtcccag ccgccgcgaa ttcaccggac
     4201 cgaacaggta tttcatcgtt tcccctatac ctcgccggtg atctgaaggc ggttgagttc
     4261 gctgatcttg ccgatgagga tctcgtcggt cttcgccagg cgctgcgcca ggatggtgac
     4321 catcttcgcg gcgaagctgg ggtttttcag cagaaacgcc ttgagctgcg caaggttgtc
     4381 gatgtaggca agctgcacgt tgcccaccgc gtgggcgttc gcgctgcgcg gcgtgttggt
     4441 aaacagcgat atctcgccga agaaatcgcc cttcttgagc gttgccacgg caaagcggct
     4501 cttgccgtcc gtgaggctaa tctccacctc gccctcgagg atgatgaaca tccgctgctc
     4561 ggtctttccg ctttcgatga tccgctcggt atttgtatac tgcagaacgc ggcccgccat
     4621 gtcccctcct cgccgcgacg cacttcgccc cgacggcatt gtcccgcaca gtggatgatt
     4681 agaaaaacgc gcgggaaaaa agcaattgaa attttccgac gcacatcgga tgcttgcgcg
     4741 ccgtcgaagg cgacccccga aggagatcat cacccgacat ggaacggttc tttcatcccc
     4801 gctcgatcgt gctctttggc gcgagcgaaa atcccggcaa gggcgggaac caggtgctgc
     4861 agaacttccg gcagtacatg gcccgaaacg gctacccgga cctcttcatc gtacacccgc
     4921 gcgcctcgtg catcaatggc gttgcctgct atcgctccct cgacgagctc ccgcgcaacg
     4981 cgaacggcga cacccgcctg gacctcgcgg tgatcgtgct cccggtcccc gaggtgatgg
     5041 cggccgtccg cgcctgcgtc gcgcgcgagg tccgcggcat cctcatcgaa tccggcaacc
     5101 tctcgaacga cgccgacgag gccgcccgct tcgccgcgga gcttcgcgcg ctcattcgcg
     5161 gacgcgacat ccggatcgtg gggcccaact ccatcggctt caacgtgccg gaccttcgct
     5221 actgcacgcc catcaagtac tacagccagt tcctggaatc gcgcgagcgc aatgtctcca
     5281 tcgtgggcca gtcgggactg ttcgtgacgg gcttcgtgga atggttcttc gatgcggcgg
     5341 gccacggcca gcccttcggc gtgtcgaaca tcgcggccat cggaaacaag ctggacgtga
     5401 acgagtgcga cctgctggaa tactatctcg ccgacgcgaa gacgcacgcc gtgggcatgt
     5461 acctcgaaga catccgcgac ggcgcgcgct tcgcggacat cctccgggat aaccgcgccg
     5521 ggagacaaaa gcccatcgtc ctcctcaagg ccggcaagag cgagaaaggg aagcaggccg
     5581 tctcgtcgca caccggatcg ctcgcgggca gctacgccgt ggtcgaggcg atcgcgaaac
     5641 agtacggcat gatgctcgtg gagacctacg aggagctctt cgagatgctg tccgtcgtgt
     5701 gccagttccc gcgcatgcgg ggcgggcgcg tcggcgtgat ctcggtctcg ggatcgggct
     5761 gcgtcctctc gtccgatttc gccgagaagt acggcatcga gctgccggag ctcgacgggg
     5821 cgaccgtcgc gcggttgcgc ccgctcttcc ccgactgggc ccccatccac aaccccatcg
     5881 acacctgggc ctcggtcgag aaggtgggcc ccgaggaaag cttcagccgc atcctcgaca
     5941 tcttcatcga atcgggccgc ttcgactgca tcctgatgat gaccatcagc tcggccttcg
     6001 cggcgttcga ctggcaacac atcggcgccg tgcagcgaca gcatcccggc atccccatca
     6061 tcgcccactt cttcggcggc cagtccatca acgagaacat gtcgttcgca atgaaacaca
     6121 gcatcccggt gattcccaac ttcgagcaca tcttcaaact gctggggatc ctgcgggact
     6181 attcgcgacg gatggacagg gccgggaaat tccgggacgg cgcgtcaggg tctcgcggcg
     6241 gatgatgccg atgatccagc ttcgaaaatg aaccggcgca tgtcctccgg caccggcgcc
     6301 tcgaagcaca ggcgctcccg cgtcaccgga tgatcgaagg aaagccgcgc ggcatgcagc
     6361 agatggcgcg gcgcctcgag ccacggccgc ggatcatact cgcccgcctt gacggcatgc
     6421 acgtaggcaa ggaactcctc gtcggtgcgc ccgtagagct tgtcgcccgc gatcgggtat
     6481 ccggcgtgcg cgaggtgcac gcggatctgg ttggtgcgcc cggtgagcgg cacgcagcga
     6541 agaagcgtga aacgctccag gcgctcgatc acctcgaagc gcgtcaccgc gcgcttcgcg
     6601 cctggctcct cgtcatcaac caccctcttc tttatgctga tgccgctcgc gcggtcgggg
     6661 acgatatagc cccccacctc gaaggcatcg gccgccacaa cgccgcgcac gatcgcgcga
     6721 tactcctttt ccaccgtccc ggcgccgaac tgccgcatga gccctcgatg ggccgattca
     6781 attttcgaca cgagcatgac gccgctcgtc tcgcggtcca accggtgcac cagattgagc
     6841 ttgcccgcga aaccctgggc ggcatatcgc tcgcggatca tccagatgaa ggtgttcttg
     6901 atgaagcgtc cgtcggcgtg cgccgggaga ttgccgggct tgtcggcgac gaggaacgac
     6961 tcctcgtcgt ggaggatcgc gatattcccg tccaccggcg gctcctcgag gaggatggta
     7021 tacgcgacga gatcgccctt ggccagggga cggtccgcgt ccgacgcgct cccgttcacc
     7081 actacctttc cggcggcgat gagcgccgcc cactcctcgc gaccgcggta gc
//
LOCUS       DAORHF010000192         7102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10344_length_7102_cov_5.520221, whole genome shotgun sequence.
ACCESSION   DAORHF010000192 DAORHF010000000
VERSION     DAORHF010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10344_length_7102_cov_5.520221"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1357
                     /locus_tag="PKJ16_13950"
     CDS             <1..1357
                     /locus_tag="PKJ16_13950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48143.1"
                     /translation="DDALGALLTYVTSQSMQEVFNLRIVARVSSALETYEPFTHNNTK
                     TLGLSLVPDLSVLMIDPKIRYEWGWGQNFIFDNIGTFGTGLHLNSYFQIDPSGIDAKA
                     SCSVPVWVFRYLGNFMYAEARASYSFFDAGTYPSGFFDGFEMLEGYGPRYTTGMNFKF
                     EFFGNSLYSFNSNGPFKWGMGDLGATAPADFINIPSISFDVDPEIDFDPGIPSNSFGT
                     NLSPSALSYSKSYSIEQTVWIGPVPVTVEAGIYGEIGVNTFIFVGHNMRAGVIPYAKI
                     GAYASAGVGVPGFSAGVEGKVDLITASMPVLGWFNPGFTVHQDAETKQIALLIDARFK
                     EQIRLHLVGPNGVISLYAKYPTIEWCRKRICAWKCWTIKFPCGIDIKKATKPLVRFGP
                     AFVVDKTLFSAEQSKTIILGKFPMPVLPEPDPELPPETGDGDDAEPPVEVPGDGEGSE
                     G"
     gene            1605..3317
                     /locus_tag="PKJ16_13955"
     CDS             1605..3317
                     /locus_tag="PKJ16_13955"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV48144.1"
                     /translation="MYSFEMNTHVVTRNLLTGMFGSFSQGISGTLNMKVLSAEEGAVS
                     AVMQLHPVSVVMGEKNDPNMERLFSMVFMVTFDRSGKMKKLMFDNSISEEDEKMVANL
                     VRALEVAVPGTILSTWKESGEDENGAFESRYKESKTNILKRKIAYVRKPKTRTRHGRD
                     VAIRVARSAFRFQVDREGSWFTYLRGREQLEISEPQTKSVQVSTGIELDVARVPFEPD
                     PGARIWSLSDSASVKRALGAGSKNPVSLRTEMRLAALDRQMGGVTLDKVLAGVAGEKK
                     IPFSAIQLLRDYLLMKPDDALKIPDLLQRGGISSSRSAAIIHALGLAGTPQAQQALIG
                     IMNNPIHDTMNRMRAVSELGSVVHPAAGTIDELWRTAAIRGDRESAELANTTLLALGG
                     LSAVQRDIDDKVSSEINARLVDMAKNAIDTKEASLALQAIGNTRDSSLSDAVVGYLGS
                     DSPEIRSAAAYALRNMDDEGSLNALSKMLSKEENPQVKQAVVGSMLNRPATADTMKSM
                     SRQTLRETDDSTRHVMYQYLVKNRERYPDVKETLAKCLDIERSPQARVLLYKGIYSKT
                     KSPR"
     gene            3434..4171
                     /locus_tag="PKJ16_13960"
     CDS             3434..4171
                     /locus_tag="PKJ16_13960"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV48145.1"
                     /translation="MRKSSFISCIAVCMALAFVAAAHADIKVASVSGTVAFKADRQWR
                     PLTRGMTLREGTKVSTGARSTAEISIDGATLTVRPLTMIKISRNAVSKDASDTKIALK
                     YGGVNAKVPKLKTVKSVFKIQTPVATSSVRGTEQEVFFGGMSGMTIRVPEGTVDAENR
                     NGSERSLRGKKNYRQRPDRSLPDNILSDVRDGALVRVHADDITDEEKQLHEYFDDYLD
                     NGDRPDRFSTIMNKQKATLKIRIIWPY"
     gene            4182..4682
                     /locus_tag="PKJ16_13965"
     CDS             4182..4682
                     /locus_tag="PKJ16_13965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48146.1"
                     /translation="MKRLFAAVCIIMLSLPVLAQDGASVKIYKSRNPGEQGLGIIEFI
                     AQVSSRDRVTCGDALNFFVMVIGREPGTFDENKAALKTAGIDAELGMGENDPVRKGDV
                     ALMAAEYLKLGDLAMYAIFKTKRYAFRACVANGIMDYAGSEWDRMSGAELIEVMNRVS
                     RRAESK"
     gene            4728..6116
                     /locus_tag="PKJ16_13970"
     CDS             4728..6116
                     /locus_tag="PKJ16_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HNV48147.1"
                     /translation="MNLRRVAILWCVCMALASGAYAQRSENADRDDSFYPDFRSDEDM
                     RAQRFTREPFSISYGGWLMPMVIDERRNDQVLTTSVNTAKLWIKSTLWGKSYVYVRGK
                     DQYYHILKHDGIGIREAGGSDGIKLNENKVDLDVGYLAVSTDDNMLQVYAGRKFFILG
                     SGLALNGRGDGIQVDLNTSVADVRVFGMYTGLLQKDFNPYGLSDEDIAVGARRYFAGS
                     MVQRTLWNQTLYLFAMAQLDNGRQQFIRSRYNSQYYGIGLKGVITDSVEYFGEFVYES
                     GESYLKGKNEKRNVNAAAAQAGVNWYLPLPLEPVFMVQYAYGSGDEDRYDCASPTANQ
                     AGEDNGFIYFGTFLGGYALRPLLSNLHIARGGFSLSPFSWSESRFLRRMNVTAKYSYY
                     MKDKPKGVINYNEAPLEETEVGHGADASFRWRIFYDLSIFVNYAMFFPGKAYVEGEKN
                     RQFIQGGCNFIF"
     gene            6192..>7102
                     /locus_tag="PKJ16_13975"
     CDS             6192..>7102
                     /locus_tag="PKJ16_13975"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV48148.1"
                     /translation="MISSISAVREMLLKKLAPLWSAVEKSRLGAAGVALAMFALVFAF
                     SFSRPYEHLELTLYDIRFRIKPSTPQWEHLTFVDMEDNSIEQIGKFPWPRNVYTRGLD
                     VMRSAGSRLTAFDIEFPNESPVTVDAGVMKALKSRTGAKAMRGMLDKVIVNNDEVFAG
                     GLARYGRGILPFSFQNMELKESESARLRRAEIARARAQFRERASVPVPPERRGEFAAL
                     VDPARMDILYPIPELIASARGFGFVDSDFDVDGVARKVRLVRVFKDRLYFNLSLMMLM
                     DLCGVAKEQVAVFPGSRIVLANAWNPRT"
BASE COUNT         1459 a         2024 c         2230 g         1389 t
ORIGIN      
        1 ggatgacgct ctcggggcgc ttctgaccta tgtgacctcc cagtcgatgc aagaggtgtt
       61 caatcttcgg attgtcgcac gcgtatcgag cgcgcttgaa acctacgagc cgtttacgca
      121 caacaatacg aagacgctcg gattgtcgct cgttcccgac ctttcggtcc tcatgatcga
      181 cccgaagatc cgctacgagt ggggctgggg gcaaaatttc attttcgaca atatcgggac
      241 cttcgggacc ggccttcatc tgaactcgta cttccagatc gatccgtcgg ggattgacgc
      301 gaaggccagc tgcagcgttc cggtgtgggt gttccggtac ctcggaaatt tcatgtatgc
      361 tgaagcgcgc gcgtcgtata gtttcttcga cgcgggtact tatccgagcg gtttcttcga
      421 cggcttcgag atgctggaag ggtacgggcc tcggtatacg accggcatga acttcaagtt
      481 cgaatttttt gggaattccc tgtattcctt caattcgaac ggtccgttca agtggggcat
      541 gggcgatctc ggtgcgacgg cgcccgctga cttcatcaat attccgtcga taagcttcga
      601 cgtcgatcct gagatcgact tcgatccggg cattccgagc aattccttcg gaacaaacct
      661 ctcgcccagc gcgttgagct actccaagtc gtattcgatc gagcagaccg tttggatcgg
      721 tcccgtcccg gtcactgtcg aggccggcat ttatggcgag atcggcgtca atacgttcat
      781 cttcgtggga cacaacatgc gcgcgggcgt catcccgtac gccaagatcg gcgcgtatgc
      841 gagcgccggt gtgggcgttc cgggattcag cgccggtgtc gaaggcaagg tcgacctcat
      901 taccgcgagc atgccggtgc ttggttggtt caatccgggc tttacggtgc atcaggatgc
      961 ggaaacgaag cagattgcgc tcctcatcga cgcgcgattc aaggagcaga tcagactgca
     1021 cctggttggc cccaacgggg tgatatcgct gtacgccaag tacccgacca tcgagtggtg
     1081 cagaaagaga atttgcgcat ggaagtgttg gacgatcaag ttcccgtgcg gcatcgacat
     1141 caagaaggcg accaagccgc tggtccggtt cggccctgca ttcgtggtcg acaagacgct
     1201 cttctcggcc gagcagagca agacgatcat tctcggcaag ttcccgatgc cggtgctgcc
     1261 tgaacccgat ccggagctgc cgcccgaaac cggagacggt gacgatgccg aaccgccggt
     1321 cgaggtccct ggcgatggcg agggttccga aggctgagtt gagcgcgact accgcatgta
     1381 acgcgtatcg gtaatcggta gaaacgacga atcgaaggca ggctgtaaac attgtttatg
     1441 gcctgtcttt ttttgggaat acctcaggag ctgtgaatat gaaaatacca tccgttcggc
     1501 gttcaacgat cttgattgga atcggcgtcc tcgcggggtt ggcgattatc gtctttatcg
     1561 cgctgccgcg aacgagattc caatggcgtg ccggcgaccg tcacatgtat tcgttcgaga
     1621 tgaatacgca cgtggtgacc aggaaccttc tcacgggcat gttcgggagc ttctcgcagg
     1681 gcatatcggg gacgttgaac atgaaggtcc tctccgccga ggagggtgcg gtgagcgcgg
     1741 tcatgcagtt gcaccccgtg agcgtggtga tgggcgaaaa gaacgatccg aacatggagc
     1801 ggcttttctc catggttttc atggtgacgt tcgatcggtc ggggaaaatg aaaaaactca
     1861 tgttcgacaa ttccatctcc gaggaagacg aaaagatggt cgcaaacctt gtccgcgccc
     1921 tcgaggttgc ggttccggga acgatcttga gcacatggaa ggagtccggc gaggacgaga
     1981 atggggcctt cgagtcgcgg tataaagaat cgaaaaccaa tatcctgaaa cggaagatcg
     2041 catacgtgcg caaaccgaaa acgcgcactc gccacgggag ggatgtcgcg attcgtgtcg
     2101 cgcgctcggc gttccggttc caggtcgacc gtgaggggag ctggttcacc tatctgcgcg
     2161 gacgggagca gttggagatt tcggagccgc agaccaagtc ggtgcaggtg agcaccggga
     2221 tagagttgga tgtcgcccgc gttcccttcg agccggaccc cggggcgcgc atatggtcgt
     2281 tgagcgattc tgcttcggtg aagcgcgcgc ttggcgccgg atcgaagaac ccagtctctc
     2341 tgcgcacgga aatgcgtctt gccgcgctcg atcgacagat gggcggtgtg acgcttgaca
     2401 aggtgctggc cggggtcgcc ggcgaaaaga aaataccgtt tagcgcgata caactattgc
     2461 gcgattatct cctcatgaaa ccggatgacg ctttgaagat tccggacctg ctccagcggg
     2521 gcgggataag ctcgagccga tccgccgcga tcatacatgc gcttggactg gccggaacgc
     2581 cgcaggcgca gcaggccctc atcggcatca tgaacaaccc gatacacgac accatgaacc
     2641 ggatgcgggc ggtttcggag ctggggagcg tcgtgcatcc cgcagcggga acgattgacg
     2701 agctctggcg caccgcggcc atccgcggcg atcgggagag cgcggaactg gcgaacacga
     2761 cattgctcgc gctgggaggc ctctcggcgg ttcagcgcga tattgacgac aaggtctcgt
     2821 cggagataaa tgcccgtctc gtcgatatgg cgaaaaacgc gatcgacacg aaggaggcgt
     2881 cgcttgcgct ccaggcgatc ggaaatacgc gcgacagctc gctctcggat gcggttgtgg
     2941 gctatctcgg ttccgacagc cccgagattc gttccgccgc cgcatatgcg ctgcgcaaca
     3001 tggacgacga gggttcgctc aatgcgctct cgaagatgct ctcgaaagag gagaacccgc
     3061 aggtgaagca ggcggtcgtg ggaagcatgc tcaatcggcc cgccacggcc gatacgatga
     3121 aatccatgag ccggcagacg cttcgtgaaa ccgacgacag cacgcgccat gtcatgtacc
     3181 agtacctggt gaagaaccgc gaacggtatc ccgatgtgaa agagacgctt gcgaaatgcc
     3241 tcgatatcga acgatcgcca caggcccggg tgctgttgta taaaggcata tattcgaaaa
     3301 caaaaagccc acgatgactt ttttggtaaa gcccaatggg gaaattttat ttttttgatt
     3361 gtaaagaatt gaattgtgca gccatcatac atacgttgaa tggaggcgcg gctgcttccg
     3421 ggaggtttac gccatgagaa aatcatcgtt tatatcgtgt atcgccgtgt gcatggcgct
     3481 tgcgttcgta gccgccgcgc acgccgatat caaggtggcc tccgtaagcg gcacggtcgc
     3541 cttcaaggcg gaccggcagt ggcgcccgct cacgcggggg atgacgctcc gcgaggggac
     3601 caaggtaagc accggggcgc ggtcgaccgc cgagatatcc attgacggcg cgacgctcac
     3661 cgtccgcccg ctcaccatga taaagatctc gcgcaatgcc gtctccaaag acgcgtccga
     3721 tacgaagatc gccctgaagt acggcggcgt gaacgcgaag gtcccgaagc tcaagaccgt
     3781 caagtcggtg ttcaagatcc agacgccggt cgcgacctcc agcgtgcgcg gtacggaaca
     3841 ggaggtcttc ttcgggggca tgtccggcat gaccatccgc gtccccgagg ggacggtgga
     3901 cgccgagaac cggaacggga gcgaacgcag tctgcgcgga aagaagaact accgacaacg
     3961 gcccgatcgc tcgcttcccg acaatatcct ctccgatgtg cgcgacggcg cgctggtccg
     4021 cgtgcacgcc gacgacatca ccgacgagga gaagcagctg cacgagtatt tcgacgatta
     4081 cctggacaac ggcgatcgtc ccgatcgctt ttcgaccatc atgaacaagc agaaggcgac
     4141 actcaaaatc aggatcatct ggccgtactg agggaatcgc gatgaaacga ttgttcgcag
     4201 ccgtgtgcat catcatgctg tcgctgccgg tcctggcgca ggacggcgcg tcggtgaaga
     4261 tttataaaag caggaacccc ggcgagcagg ggctcggtat aatcgagttc atcgcgcagg
     4321 tgtcctcgcg cgaccgggtc acctgcggcg acgccctcaa ttttttcgtt atggtcatcg
     4381 gacgcgagcc ggggaccttc gacgagaaca aggcggcgct caaaaccgcc ggcatcgatg
     4441 cggagctggg gatgggcgag aatgatccgg tgcgaaaggg cgacgtggcg ctcatggcgg
     4501 ccgagtacct gaagctcggt gatctcgcga tgtacgcgat cttcaagacg aaacgctatg
     4561 cctttcgggc ctgcgtcgcc aacggcatca tggactacgc cgggagcgag tgggaccgca
     4621 tgagcggcgc cgagctcatc gaggtgatga accgggtctc gcgccgcgcc gaatcgaaat
     4681 agagagcacg aacgtatcgc aaacagcagc aaggagcacg cagggacatg aacctccgaa
     4741 gagtggcaat actatggtgt gtgtgcatgg cgctcgcgtc cggcgcgtac gcacaacggt
     4801 ccgagaacgc cgaccgtgac gattcgtttt atcccgattt ccggtcggac gaggacatgc
     4861 gcgcgcagcg gttcacgcgc gagccgttct cgataagcta cggcggttgg ctcatgccca
     4921 tggtcatcga cgagcgacgg aatgatcagg tcctcacgac gtcggtgaac accgcaaagc
     4981 tatggatcaa gagcacgctc tggggtaaga gctatgtcta tgtccgcggg aaggaccagt
     5041 actatcatat cctcaagcat gatgggatcg gtatccgcga ggccggcggt tcagacggaa
     5101 ttaaactaaa cgaaaacaag gtcgacctcg acgtgggcta tctcgccgtg tccaccgacg
     5161 acaacatgct gcaggtgtac gcgggccgga agttcttcat ccttgggagc ggccttgcgc
     5221 tcaacgggcg cggcgacggc atccaggtcg acctcaatac cagcgtggcg gacgtgcgcg
     5281 tgttcggcat gtatacggga ctgctgcaga aggacttcaa tccctacggt ctcagcgacg
     5341 aggacatcgc ggtgggcgcc cggcggtatt tcgcgggaag catggtccag cgaacgctgt
     5401 ggaaccagac cctgtatctg ttcgcgatgg cgcagctcga caacgggcgc cagcaattca
     5461 tccggagccg ctataattcg cagtattacg ggatcggcct caagggcgtg atcaccgact
     5521 cggtcgaata cttcggcgag ttcgtatatg agagcggcga aagctatctg aaagggaaaa
     5581 acgagaagcg gaacgtgaac gcggccgcgg cgcaggccgg ggtcaactgg tatctgccgc
     5641 tgccgctcga gccggtattc atggtccagt acgcctacgg ttcgggcgac gaggaccggt
     5701 acgattgcgc atcgcccacg gccaatcagg cgggcgagga caacggcttc atctacttcg
     5761 ggacatttct cggcggctac gccctccggc cgcttctgtc caatctccat atcgcgcgcg
     5821 gcggtttctc tctgtcgccg ttttcctggt ccgagtcgcg cttcctgcgg cgcatgaacg
     5881 tcacggcgaa atattcctat tacatgaagg acaagccgaa gggcgtcatc aactacaacg
     5941 aggcgccgct tgaggagacc gaggtcggcc acggcgccga cgcgagcttc cgctggcgca
     6001 tcttctacga tctctcgatc ttcgtgaatt acgccatgtt ctttcccggc aaggcctacg
     6061 tggagggcga gaagaaccgg cagtttatcc agggcggctg caacttcatc ttctagtttc
     6121 gcgcatgcgg tttcgcccgt ccggcgtggc cgtcactgcc atgccgccat cttcccatcg
     6181 gggttttcaa tatgatatcg tccatctccg ccgttcgtga aatgctcctg aaaaaactgg
     6241 cccccctctg gagcgccgtc gagaagagcc ggctcggcgc ggcgggggtc gcgctggcga
     6301 tgttcgcgct ggtgttcgcc ttctcgttct cgaggcccta cgagcacctc gagctcacgt
     6361 tgtatgatat ccgtttccgg atcaagccgt cgacgccgca gtgggagcac ctcaccttcg
     6421 tggacatgga ggacaacagc atcgagcaga tcgggaagtt cccctggccg cggaacgtct
     6481 acacgagggg cctcgatgtg atgcggtcgg cgggatcgcg gctcacggcc ttcgatatcg
     6541 aattccccaa cgagtcgccg gtcacggtcg acgccggggt catgaaggcg ctcaagtccc
     6601 ggacgggcgc gaaggcgatg cgggggatgc tcgacaaggt gatcgtgaac aacgacgagg
     6661 tcttcgccgg cggtctggcg cgctatggcc gcgggatcct cccgttttcc ttccagaaca
     6721 tggagctcaa ggagagcgag tccgcgcggc tccggcgggc cgagatcgcc cgggcgcggg
     6781 cgcagtttcg cgagcgcgcg tcggtgccgg taccccccga acggcggggt gaattcgccg
     6841 cgctggtcga tcccgcgcgc atggacatcc tctacccgat cccggagctc atcgcatccg
     6901 cgcgcggttt cgggtttgtc gacagcgatt tcgacgtgga cggggtcgcc cgcaaggtgc
     6961 gcctggtgcg cgtgttcaag gaccggctct acttcaatct ctccttgatg atgctcatgg
     7021 atctctgcgg ggtcgccaag gagcaggtgg cggtgttccc cggatcgcgg atcgttctcg
     7081 cgaacgcgtg gaacccgcgc ac
//
LOCUS       DAORHF010000193         7100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10350_length_7100_cov_6.273101, whole genome shotgun sequence.
ACCESSION   DAORHF010000193 DAORHF010000000
VERSION     DAORHF010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10350_length_7100_cov_6.273101"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PKJ16_13980"
     CDS             complement(<1..282)
                     /locus_tag="PKJ16_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48149.1"
                     /translation="MDVDNPINHYVEPVEIEIYLKKAGRVRTIIKDLFIELIEVEPAN
                     EHSVKIFAHFRERNEPIDLMEVMNLFPEYITPIFDSYYQYIALFEKVSMH"
     gene            complement(269..2557)
                     /locus_tag="PKJ16_13985"
     CDS             complement(269..2557)
                     /locus_tag="PKJ16_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48150.1"
                     /translation="MALTLDDIIKTERDRLFVIDRECFIVFTGDSLDDEKPFIRIGTY
                     VDLPVEIIPLVENIVITDTMVGNPAIEQFNIDITYLDTNRFIGSRAIVRRYLEFQKVF
                     GLDLGAAKVVDIDTDIPDISNERLISNRDSFVGIFYKNGNFKIIHNNRDIFNLQEIDR
                     EGNQDIARAHDALSEAFRGTARYRGCGLCVIDNNPLLYRKGAFAAYQFPKRYYERFAA
                     LEIDPQSIKDIALPAANLLHVAGFLKWKHARAGRIRIFSDNREGTGFAQRLFREASIA
                     AADFTGFSADAGGARITHYPGGFNIKVVFPAVEPARADVSVAYCKGRAGIETIVRERL
                     DAILVNYSVFEDANLIFKSTDTPLAIIDDGSKNISRLPEDGRVIIRQGVQYEFRACGD
                     FDELARSMADDYGLGGAAETIASGDAGAIGALFADADPARAANLRSAIRAHIDTVTDR
                     RLAGSLRKILQEHAAPSGALDAAGGRVAHLVFFRRRLYQFILPPPERTVSPVSLVDEI
                     RADDAPRHQSLPDRKVGSFYERIVDDRRRLDELLRLYAERHKPEPWLGELREAIELRK
                     REFFEDDLERANVELARAAQKKKMIRLAKMSGVIAAIALGIVLATVTAVNVYRVHKER
                     ERLALEERERARIIAAYGIRISDTDIFNYANAIAVKNGYNRISLAHIRGKNPNWIYPK
                     NVFVLPDGERITVAEGDTLWGIAHRKLLQANIDFYRAIELATAKRTRGESAAEEIARA
                     TSLALTPKHRELVEALKRDGRR"
     gene            complement(2563..4272)
                     /locus_tag="PKJ16_13990"
     CDS             complement(2563..4272)
                     /locus_tag="PKJ16_13990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HNV48151.1"
                     /translation="MESFGRNEIINLFASIALLLAAARVLGEAFRRFRLPAVVGELLA
                     GVLLGPTVFGRIFPGAFAALFADGSPSTIALDGFVLLGVTFLLLIAGLEVDLSSVGKL
                     GRHVAFISVMTIALPFGIGVATAALFPTLFGMYRSDPVFSLFLGVALSITALPIIAKI
                     LMDINLLQSEIGVIILAAALVNDIAGWIVFSIIIQLMKTGSVGVAEVVRMIVLTALVT
                     GFVLTAFRWLVNKIIPWVQANTEVPGGVLAFIVITGLLVSTLTEAIGIHAIFGAFLAG
                     IAFGDSPHLKQHTREVIHQFINNIFAPLFFVSIGMKVDFAQHFNPLLTAVLIVIAFAT
                     KVAGAMLGGKWAGLRLRQATAIGWGMSARGAMEIILGLVAMQHGLISRDIFVSITIMA
                     VVTSLLSAPLMKAFMRTTGARGILDLSDRRLFIGALKATNAREAIGELAALAGERLGI
                     DPNLIAETALERERIMSTGIGDGVAVPHARLTQIRAPRVAIGMSPNGIDFNAPDGKPA
                     RIVFLILTPENDQEAQIDVLSEIARLLGRAWTRDPVLRAHRYSEFVTAIKMARFGAKL
                     SEG"
     gene            4437..5426
                     /locus_tag="PKJ16_13995"
     CDS             4437..5426
                     /locus_tag="PKJ16_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HNV48152.1"
                     /translation="MSRQLLEYHSVIGYRFIPGLKARVEHEAGGYLVRTNGAGFRCDR
                     EFLPARGPSRRALLFGDSYTAGDGVSNGKRYGDLLETMVPNLEVYNFGLSGSGTDQQY
                     LAYREFARGIERDLVIIAVFVENIMRVNARYRFFRNENNEEVLYEKPYFSLGAEGLTL
                     HGVPPERRPLREMDLPPDARSRIDAGGRFASLRLLARRLGIKDLAQRITRYNPVVEYN
                     RPDDPRWLLMRALLAEWIREIGGPVLVVPVPLYQFIEGTSDPSCYQARFAELARDTGC
                     HLHDPLPDLKAYPKEERRAFRFARDVHYTPAGHRALAASLAPAVQSILDAAGS"
     gene            5430..>7100
                     /locus_tag="PKJ16_14000"
     CDS             5430..>7100
                     /locus_tag="PKJ16_14000"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HNV48153.1"
                     /translation="MSTTILGISSFYHDSAAALVRDGEIVAAAQEERFSRKKHDPRFP
                     RHAINYCLEEAFIEADELDAIVFYDNPLLTWDRVVKNCVMAGEPAFEQFEKAAASILG
                     IKLWVQDYARRTLGCLGRSGRVLFTDHHFAHAASAFYPSPFREAAILTLDGVGEWDTT
                     AIGRGDDDGIELAQVIEYPHSLGLLYSAFTYFCGFKVNSGEYKLMGLAPYGRPVYERI
                     IRDNLIDLKDDGSFRLNMEYFGYLDSFRMTNERFDALFGGPPRQPESRITRREMDLAM
                     SVQAVLEYAVLRLARHARRESGAPNLVMAGGVALNCVANGKLLREGIFDHIWIQPASG
                     DAGGALGAALMAAHGYFGSPRRMSPGGRDTLKGSYLGPAYSSAEVRAFLERHGYPYER
                     IEDPDGRARTVAQALADGKVTGFLSGRMEFGPRALGARSIIGDPRSETMQSTMNLKIK
                     YRESFRPFAPSCLAENAAEYFDLDVESPYMLLVAPVREERCIPFDREAFYNNEDMLQI
                     INQARSDLPAITHVDFSARVQTVHPDDHPDYYRLIRAFREITGFGVIIN"
BASE COUNT         1193 a         2245 c         2425 g         1237 t
ORIGIN      
        1 gtgcatgctc accttctcga agagcgcgat gtactggtaa taggaatcga agatgggggt
       61 gatgtactcg gggaagaggt tcatcacctc catgaggtcg atgggctcgt ttcgttcgcg
      121 gaagtgcgcg aagatcttca cgctgtgctc gtttgcgggc tccacctcga tgagctcgat
      181 gaagaggtcc ttgatgatgg tgcgcacgcg accggccttc ttgagataga tctcaatctc
      241 gaccggctcc acgtagtggt tgatggggtt atcgacgtcc atcgcgtttc agggcctcga
      301 ccagttcccg gtgcttcggg gtgagcgcca gcgacgttgc gcgcgcgatc tcctcggccg
      361 cggattcgcc gcgcgtccgt ttcgccgtcg ccagctcgat cgcgcggtag aaatcgatat
      421 tcgcctggag gagcttgcgg tgcgcgatcc cccacagcgt gtcgccctcg gccacggtga
      481 tgcgctcgcc atcggggagc acgaagacgt tcttcgggta aatccagttc gggttcttcc
      541 cccggatatg cgccaggctg atgcggttat agccgttctt gaccgcgatc gcgttggcat
      601 aattgaaaat atccgtgtcg gaaatgcgga tgccgtacgc ggcgatgatc cgcgcccgct
      661 cgcgctcctc gagggccagt cgctcccgct ccttgtggac gcgatagacg ttcaccgccg
      721 tgacggtcgc caggacgatc ccgagggcga tcgcggcgat caccccggac atcttcgcga
      781 gccgaatcat ctttttcttc tgggccgcgc gggcgagttc gacgttcgcg cgctcgaggt
      841 cgtcctcgaa gaattcgcgc ttgcgcagtt ctatcgcttc gcgcagctcg cccagccagg
      901 gctccggctt gtggcgctcg gcgtagaggc gcagcagctc gtcgagccgc cgacggtcgt
      961 ccacgatgcg ctcgtagaat gatcccacct tccggtccgg gagcgactgg tggcgggggg
     1021 cgtcatcggc gcggatttcg tcgacaaggc tcaccggcga cacggtccgc tcgggaggcg
     1081 ggagaatgaa ctggtacagg cgtcggcgga aaaacacgag atgcgccacg cgaccgccgg
     1141 cagcatcgag cgcgccggac ggggcggcgt gctcctggag tattttccgg agggaccccg
     1201 ccagccggcg gtccgtcacg gtgtcgatat gcgcccggat cgccgagcga agattggcgg
     1261 cgcgcgcggg atcggcgtcc gcgaagagtg cgccgatcgc gccggcgtcg cccgaggcga
     1321 tggtctccgc tgcgccgcca agcccgtagt cgtccgccat cgaacgggcg agctcgtcga
     1381 aatcgccaca tgcgcgaaac tcgtactgga cgccctgccg aatgatcacg cgcccgtcct
     1441 cggggaggcg ggagatgttt ttgctgccgt cgtcgatgat cgcgagcggc gtgtcggtcg
     1501 acttgaagat gaggttcgcg tcctcgaaca cggagtagtt caccaggatg gcgtccagcc
     1561 gctcgcggac gatggtctcg attcccgcgc gtcccttgca gtaggcgacg ctcacgtcgg
     1621 cgcgcgccgg ctcgacggcg gggaacacga ccttgatgtt gaagccgccc ggatagtgcg
     1681 tgatgcgcgc gccgcccgcg tcggcggaga atccggtgaa atcggccgcc gcgatcgagg
     1741 cctcgcggaa gagccgttgt gcgaaaccgg tcccctcgcg gttgtcggag aaaatgcgga
     1801 ttcgcccggc gcgggcgtgt ttccacttga ggaagccggc cacgtggagg aggttggcgg
     1861 cggggagggc gatgtccttg atcgactgcg ggtcgatctc gagcgcggcg aagcgctcgt
     1921 aatagcgctt tggaaactgg taggcggcga acgcgccctt tcggtacagg agcgggttgt
     1981 tgtcgatcac gcacagcccg cagccccggt agcgggccgt gccgcggaag gcctcggaga
     2041 gcgcgtcgtg cgcgcgcgcg atgtcctggt ttccctcgcg gtcgatctcc tgcaggttga
     2101 agatgtcccg gttgttgtgg atgatcttga agttgccgtt tttatagaag atccccacga
     2161 aggagtcgcg gttggagatg aggcgctcgt tcgagatgtc cgggatgtcg gtgtcgatgt
     2221 ccaccacctt cgcggcgccc aggtccagcc cgaagacctt ttggaattcc aggtagcggc
     2281 gcacgatggc gcggctgccg atgaagcggt tggtgtccag gtaggtgatg tcgatgttga
     2341 actgctcgat ggcggggttg cccaccatgg tgtcggtgat cacgatgttc tcgaccagcg
     2401 ggatgatctc gaccgggagg tcgacgtagg tcccgatgcg gatgaacggc ttttcgtcgt
     2461 ccagcgagtc gccggtgaag acgatgaagc actcgcggtc gatcacgaag agacggtcgc
     2521 gctcggtttt gatgatgtcg tccagcgtca gtgccatgcg gcttatccct cgctcagttt
     2581 ggccccgaag cgcgccatct tgatggcggt cacgaactcg gagtagcggt gcgcgcggag
     2641 gaccgggtcg cgcgtccacg cgcgtcccag gagccgcgcg atctcggaga gcacgtcgat
     2701 ctgcgcctcc tggtcgttct cgggggtcag gatcaaaaag acgatgcgcg ccggcttgcc
     2761 gtcgggcgcg ttgaagtcga tgccgttggg cgacatgccg atggcgacgc gcggcgcgcg
     2821 gatctgcgtg agccgcgcat ggggtacggc gacgccgtcg ccgatcccgg tgctcatgat
     2881 gcgttcgcgc tcgagggccg tctcggcgat gaggttcggg tcgatgccca agcgctcgcc
     2941 ggccagcgcg gcgagctctc cgatcgcctc acgggcgttg gtcgccttga gcgcgccgat
     3001 gaacaggcgg cggtcggaaa ggtcgaggat gccgcgcgcg cccgtggtgc gcatgaaggc
     3061 cttcatgagc ggcgcggaga gcagcgaggt gaccacggcc atgatggtga tggacacgaa
     3121 gatgtcccgg gagatgaggc cgtgctgcat cgcgaccagg cccaggatga tctccatcgc
     3181 gccgcgcgcg ctcatccccc agccgatcgc ggtcgcctgc cgcaggcgga gcccggccca
     3241 cttgcccccg agcatggcgc cggcgacctt ggtcgcgaac gcgatcacga tgaggacggc
     3301 ggtgagcagc ggattgaagt gctgcgcgaa atcgaccttc atgccgattg agacgaagaa
     3361 cagcggcgcg aagatgttgt tgatgaactg gtggatcacc tcgcgcgtgt gctgcttcag
     3421 gtgcggcgag tcgccgaagg cgataccggc cagaaacgcg ccgaagatcg cgtggatgcc
     3481 gatcgcctcc gtgagcgtcg aaacgagcag gcccgtgatg acgataaagg cgagcacccc
     3541 gcccggcacc tcggtgttcg cctggaccca cgggatgatc ttgttcacga gccagcgaaa
     3601 ggccgtgagg acgaagcccg tgaccagcgc ggtgagcacg atcatccgca cgacctccgc
     3661 gacgcccacc gagccggttt tcatcagctg tatgatgatg gagaagacga tccagcccgc
     3721 gatgtcgttc accagcgccg cggcgaggat gatgaccccg atctcggact gcaggaggtt
     3781 tatgtccatg aggatcttcg cgatgatcgg cagcgcggtg atcgacagcg ccacgcccag
     3841 gaacagcgag aagaccggat cgctccggta catcccgaag agcgtcggga agagcgcggc
     3901 ggtcgcaacg ccgatcccga agggaagcgc gatcgtcatc acgctgatga aggcgacgtg
     3961 ccgaccgagc ttcccgaccg acgagaggtc cacctccagg cccgcgatga gcaacaggaa
     4021 cgtcacgccc aggaggacga acccgtcgag cgcgatggtg gagggcgagc cgtcggcgaa
     4081 gagcgccgcg aaggccccgg ggaagatgcg tccgaagacc gtcggcccga gcaggacccc
     4141 cgccaggagc tcgcccacca ccgcgggaag gcggaaccgg cggaacgcct cgccgaggac
     4201 gcgcgccgcg gccagaagca gcgcgatgct ggcgaagagg ttgatgattt cgttcctgcc
     4261 gaaggattcc atgggcccat ttggctcatc atcgaagaaa gatagcaata aattttttac
     4321 cgcttgattt ttacgcgttc gacacgatga tagtccgtcc ctgcggggcg acggtcggat
     4381 gcgaatccga cggcgcgcgc gcagagaaca ggccgtcgtc ggacgaaggg ggagcgatgt
     4441 cgcgtcaatt gctggaatat cattccgtga tcggataccg gttcatcccg gggctcaagg
     4501 cgcgcgtcga gcacgaggcg gggggatatc tggtgcgcac caacggcgcg gggttccgct
     4561 gcgaccgtga gttcctgccg gcccggggac cgtcgcgccg cgcgctgctc ttcggcgatt
     4621 cgtacacggc cggcgacggc gtgtcgaacg gcaaacgcta cggcgatctt ctcgagacga
     4681 tggtcccgaa cctcgaggtg tataacttcg gcctctccgg aagcggcacc gaccagcagt
     4741 accttgcgta tcgcgagttc gcgcggggga tcgagcgcga cctggtgatc atcgccgtct
     4801 tcgtcgagaa catcatgcgc gtgaacgccc gctaccggtt tttccgaaac gagaacaacg
     4861 aggaggtcct ctacgagaag ccctattttt cgctgggggc cgaggggctc acgctccacg
     4921 gcgtcccgcc ggagcgccgc ccgctgcgcg agatggacct gccgccggac gcgcgatcgc
     4981 ggatcgacgc gggcgggcgt ttcgcgtcgc tgcgcctcct ggcgcgacgg ctcgggatca
     5041 aggacctcgc gcagcgcatc acccgctaca atccggtcgt cgaatacaat cgcccggacg
     5101 atccgcgctg gctgctcatg cgcgccctcc tcgccgagtg gattcgggag atcggcgggc
     5161 ccgtgctcgt ggtgccggtc ccgctgtacc agttcatcga ggggaccagc gacccgtcgt
     5221 gctaccaggc gcgcttcgcc gagctcgcgc gcgataccgg ctgccatctg cacgatccgc
     5281 tgcccgatct caaggcctat cccaaggagg agcggcgcgc cttccgcttc gcgcgcgacg
     5341 tccactacac gccggcgggc caccgggcgc tcgccgcgtc cctcgcgccg gccgtgcaat
     5401 ccattctcga cgccgcaggg agctgattca tgagtacgac cattctcggc atatcgtcgt
     5461 tctatcacga ttcggcggcc gccctggtcc gcgacggcga gatcgtggcc gccgcgcagg
     5521 aggagcgctt ctcgcgcaag aagcacgacc cgcgctttcc gcggcacgcc atcaactact
     5581 gtctcgagga ggccttcatc gaggccgatg agctggacgc gatcgtcttt tacgacaatc
     5641 cgctgctcac ctgggaccgc gtggtcaaga attgcgtgat ggccggcgag ccggccttcg
     5701 agcagttcga gaaggccgcc gcatccatcc tggggatcaa gctctgggtg caggactatg
     5761 cgcgacgaac gctcggctgc ctcggccgct ccgggcgggt gctctttacc gaccaccact
     5821 tcgcgcacgc cgcctcggcc ttctatccct cgcccttccg cgaggccgcc atcctcaccc
     5881 tcgacggggt gggggagtgg gacaccaccg cgatcggtcg gggggacgac gacggcatcg
     5941 agcttgcgca ggtgatcgag tatccgcatt cgctggggct cctctatagc gccttcacct
     6001 atttttgcgg cttcaaggtg aactcgggcg agtacaagct gatgggtctg gcgccctacg
     6061 gccgtcccgt ctacgaacga attatccgcg acaacctcat cgaccttaag gacgatggct
     6121 cgttccggct caacatggag tacttcgggt atctggacag ctttcgcatg accaacgagc
     6181 gcttcgacgc cctcttcggc ggtccgccgc gccaaccgga atcgcgcata acgcgacggg
     6241 agatggacct cgcgatgtct gtgcaggcgg tgctggagta cgcggtgctg cgcctcgcgc
     6301 gccacgcccg gagggaaagc ggcgcgccca acctggtgat ggccgggggc gtggcgctca
     6361 actgcgtggc caacggcaaa ctgctccgcg aggggatctt cgaccatatc tggatacagc
     6421 cggcctcggg cgacgccggc ggcgcgctgg gggcggcgct gatggccgcg cacggctatt
     6481 tcggcagtcc gcgacggatg agccccgggg ggcgcgacac gctcaagggg agctatctgg
     6541 ggcccgccta ctcgtcggcc gaggtgcgcg ccttcctcga gcgccacggc tacccctacg
     6601 agcgcatcga ggaccccgac ggtcgcgcgc ggacggtcgc gcaggcgctc gccgacggca
     6661 aggtgaccgg ctttctctcg gggcgcatgg agttcgggcc gcgcgcgctg ggggcgcgct
     6721 ccatcatcgg cgatccccga agcgagacca tgcagtccac catgaacctg aagatcaagt
     6781 accgggagtc gttccgtccc ttcgcgccgt cgtgcctcgc ggagaatgcc gcggagtact
     6841 tcgacctgga cgtggagagc ccctatatgc tgctggtggc gccggtgcgg gaggagcggt
     6901 gcattccctt cgaccgcgag gccttctaca ataatgagga catgctgcag atcatcaacc
     6961 aggcgcgctc ggacctgccg gcgatcaccc atgtcgattt cagcgcgcgc gtccagacgg
     7021 tgcaccccga cgaccatccc gactattacc ggctgattcg cgccttccgg gagatcaccg
     7081 ggttcggggt catcatcaac
//
LOCUS       DAORHF010000194         7064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10425_length_7064_cov_6.028107, whole genome shotgun sequence.
ACCESSION   DAORHF010000194 DAORHF010000000
VERSION     DAORHF010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7064
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_7064_cov_6.028107"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..640)
                     /locus_tag="PKJ16_14005"
     CDS             complement(140..640)
                     /locus_tag="PKJ16_14005"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HNV48154.1"
                     /translation="MAYKIVAFWGVIVILIGVGLFGLGIAYQLSKMDLERNGEIVPGK
                     ITDIVSNPPYRSPIVTFKKKDGRQVVFKSELDVNVRWFKYIKGQTVEVIYHTSTPPSY
                     SRLYYGKNPNQTYMINGFWEKNTGQLTLWAFGIIAMVGGYIMKRIFSKKAAAFAQRMG
                     PLGMSS"
     gene            complement(880..2376)
                     /gene="guaB"
                     /locus_tag="PKJ16_14010"
     CDS             complement(880..2376)
                     /gene="guaB"
                     /locus_tag="PKJ16_14010"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HNV48155.1"
                     /translation="MKKLDGFSADELFATSTGLAYNDFIILPGYIDFAPEDVILESNL
                     TRSIRLKTPMVSSPMDTVTESRMAIGLALLGGMGFIHYNNTAEEQAAEVKKVKRYENG
                     FIADPIVLSPDHTINDVIAIKERFGFSGIPITEDGRPNSRLVGIVTNRDFDFEPNHTR
                     RLGDVMTREVITARHGVSLLEANDILKKSKKGKLPIVDEAGRLVSLVCRNDLVTNREY
                     PLASKDEKKQLMVGAAISTKDDSKERLDLLVDAGVNAVIIDSAQGNSIYQIEMITYIK
                     KRHPGLDVIGGNVVTEEQCESLVRAGVDALRIGMGPGSICTTQTTMAVGRAQATAVFR
                     CARFARQHGIPVIADGGIANIGHIAKALAIGASATMMGSMFAGTDEAPGEYFYENGKR
                     LKKYRGMASLEALEKGGSKRYFSEESKIKVAQGVSGAVLDKGSLFDYMPYLIQGLKHS
                     FQDMGTRDVTRLHEMLYGNELRFELRSPSAQIEGGVHDMYSYQAPKYM"
     gene            2611..2973
                     /locus_tag="PKJ16_14015"
     CDS             2611..2973
                     /locus_tag="PKJ16_14015"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HNV48156.1"
                     /translation="MGRCKRRGLCCQDVRLDVSPEMLHNAYEFWRKSKSIDPGFSEIY
                     LIYPMLEFKFEHGSEDLPYHYRCRHFTHGADGLPACAIYEIRPRMCRDFPYYESVEHL
                     DRTGPLSPYDGCGYNDPD"
     gene            2983..3240
                     /locus_tag="PKJ16_14020"
     CDS             2983..3240
                     /locus_tag="PKJ16_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48157.1"
                     /translation="MANLLSVTVKLYSGIDKDLALTGYDPATGIGLTVARGTRLRRIL
                     KSLGMKKMSGNAYFINGARVGLWKSIREECEIACLKPSAGG"
     gene            3277..3633
                     /locus_tag="PKJ16_14025"
     CDS             3277..3633
                     /locus_tag="PKJ16_14025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48158.1"
                     /translation="MARGMIVDDSKFMRRIIKETLEEAGHDVVAEAENGFDALEMYKT
                     MKPDFVTMDITMWGKDGLKAVSEITDIDPDARIVVVSALSEKTLRLNEEGIRARAFLT
                     KPFEKSDLIGAINKML"
     gene            3668..4732
                     /locus_tag="PKJ16_14030"
     CDS             3668..4732
                     /locus_tag="PKJ16_14030"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV48159.1"
                     /translation="MSDTKFIQLYAQGGRADRAYFLEDGSVYFFASDADKYALRGRNL
                     IVGASELILAALCDTETGRLETAVADEGARLKSIGAEKFIASLSSYSFAINVCMVIAK
                     QVHLTNDIINKNLAAIDGKAKKARECAVEFYRIIERLRADFDKRRLPALRDVIVAHET
                     SLTYKRGEAFGRAVEQTVVAPNVSLEERYIEYPKDALICEEGTPGDDMFILKSGTVDV
                     YVQGNRVASIGEPGSVFGEIALLLGLARTATMKAKSGVVISRIRKQDLKEISATDGRL
                     LSDIAASLAKKHRHNIDKIASVNAMILERQIDSEAGKSEKAPLDYNRIYNDLLALKRD
                     VTALYESKKADFLQDLVDGF"
     gene            complement(4729..5259)
                     /locus_tag="PKJ16_14035"
     CDS             complement(4729..5259)
                     /locus_tag="PKJ16_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HNV48160.1"
                     /translation="MKIEITILGLLMEGNLYGYEIKKKIFERLEDYVDIKFGSIYYAI
                     KKALEHEWVKKVGTEKDGGNPERYIYQILPAGRKYFKKALKQYYEQCLIHFDIDIVLM
                     FYNTLPGDQREQFIEDRIDIIKEKLAEIRVKSEGDSRAGAEGSQEHLFSYIENHLKAE
                     LAWLKSLKKGPGADGA"
     gene            complement(5507..6091)
                     /gene="ruvA"
                     /locus_tag="PKJ16_14040"
     CDS             complement(5507..6091)
                     /gene="ruvA"
                     /locus_tag="PKJ16_14040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HNV48161.1"
                     /translation="MIATLTGTVEDLRPTDVVINVNGVGYQCAIPLSTYEALRDAPSA
                     LLYVHTLHREDQFRLFGFFTRRERELFAILLNVAGIGPALALSIISSIDGEALVAAVR
                     DGEPAMLTSVPGVGKAKAEKLIFELRRKLARLESFSREPARTPTVSADALDALVSLGI
                     DSSAAAKLVEGIVREKPDISLEDVIKSALRKPPA"
     gene            complement(6063..6563)
                     /gene="ruvC"
                     /locus_tag="PKJ16_14045"
     CDS             complement(6063..6563)
                     /gene="ruvC"
                     /locus_tag="PKJ16_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HNV48162.1"
                     /translation="MRVLGIDPGSSILGWSLLESNFHLIAYGVITPDGRDGIGGRLQI
                     IHDGLARIIDEHRPDCCAIERLFFSRNTTTAMDVAKCIGAALLTARLAGLEIAEYNPM
                     QVKQSITGYGRATKRQMQTMVMKILRLRELPRPDDAADAIAIALCHCIAHNGIRCHDR
                     NAYGNG"
     gene            complement(6564..>7064)
                     /locus_tag="PKJ16_14050"
     CDS             complement(6564..>7064)
                     /locus_tag="PKJ16_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HNV48163.1"
                     /translation="IRYEGYGPGGVAIMVDCLTDNRNRTTPEIRTIFSKNGGNLGETG
                     CVGYNFERKGVVHVDAGAIAEDNLMELLLDFDIHDIRTEDKVIVVTTSPEAYNDVYSA
                     IKNKGLATAYNEITFLPKSTVSLNEKTAQQCLRLIDLLDDHDDVQNVYSNYDIPDEVM
                     MALGEA"
BASE COUNT         1362 a         2161 c         2175 g         1366 t
ORIGIN      
        1 cgcgctcgcg caccgtccgt gacgcacgcc gcaccggcgt cgcaccccgc gccgaccgcc
       61 tcagagacgg ccgtcacggg acccggttcc tccgcgctgc cttcatcatg cgccccggtc
      121 gccgacgcgc atatcgacgc tacgacgaca tgccgagcgg tcccatccgc tgggcgaatg
      181 cggcggcctt tttcgagaag atccgcttca tgatgtatcc gccaaccatc gcgatgatac
      241 cgaacgccca cagggtgagc tgccccgtgt tcttctccca gaaaccgttg atcatgtacg
      301 tctggttcgg gtttttcccg taatagagcc gcgaatacga cgggggcgtc gatgtgtggt
      361 agatgacctc gacggtctgt cccttgatgt atttgaacca tcgaacattg acgtcaagtt
      421 cgcttttaaa aacgacctgg cgcccgtctt tctttttaaa ggtaacaatg ggcgagcggt
      481 acggcggatt ggagacgata tcggtaattt tcccggggac aatttcccca ttgcgctcca
      541 gatccatctt ggaaagctga taggcgatgc ccaggccgaa caacccaacg ccgatcagaa
      601 taacgatgac cccccagaac gccacgattt tatatgccat acttcaccct ccaaatcagt
      661 atatgtacga ataatcatac gcactcgaca tgcgctcgtc aatgattatt ttccccgcat
      721 cgtcgatgag cgacctgtgc gaagacgcgc cgagcggacg gacgcgtgcg ggaacccgac
      781 acgatccgaa accgattgca tgctgcgaaa ggaattgatt gcacgacgcg aaggggtctg
      841 gaaacaggtg aggcgactca gggccgcccc atccgttggc tacatgtact tgggcgcctg
      901 ataggaatac atgtcgtgca ccccgccctc gatctgcgcg gagggagagc gcagctcgaa
      961 tcgaagctca ttgccgtaga gcatctcgtg gaggcgcgtc acatcgcgcg tgcccatatc
     1021 ctggaaggag tgcttgagcc cctggatgag atagggcata tagtcgaaaa gcgatccctt
     1081 gtcgagcacc gcgccggaga cgccctgcgc caccttgatc ttcgactcct ccgaaaagta
     1141 ccgcttggag ccgcccttct cgagcgcctc gagcgaggcc atgccgcggt atttcttgag
     1201 ccgcttcccg ttttcgtaga agtactcgcc cggcgcctcg tcggtgccgg cgaacatgga
     1261 ccccatcatg gtggccgagg ccccgatggc gagcgccttg gcgatatggc ctatgttggc
     1321 gatcccgccg tcggcgatca cgggaatgcc gtgctgccgt gcaaaccggg cgcagcggaa
     1381 gaccgccgtc gcctgcgcgc gccccacggc catggtggtc tgcgtggtgc agatggagcc
     1441 gggccccatg ccgatgcgca gggcgtcgac gcccgcgcgg acgaggctct cgcactgctc
     1501 ctcggtcacc acgttgccgc cgatcacatc gagaccggga tggcgcttct tgatgtacgt
     1561 gatcatctcg atctgataga tggaattgcc ctgcgccgag tcgatgatca cggcgttcac
     1621 gccggcatcc accaggagat cgaggcgctc cttcgagtcg tccttggtcg atatggccgc
     1681 gcccaccatg agctgtttct tctcgtcctt cgaggcgagc ggatattcgc gattggtcac
     1741 caggtcgttg cggcacacca gcgagacgag gcggcccgcc tcgtcgacga tcggcagctt
     1801 ccccttcttc gattttttca ggatgtcgtt cgcctccagc agcgagacgc cgtgacgcgc
     1861 ggtgatcacc tcgcgggtca tcacgtcgcc cagccgccgc gtgtgattgg gctcgaaatc
     1921 gaaatcccgg ttggtcacga tccccacgag ccgcgagttg gggcggccgt cctcggtgat
     1981 ggggatcccc gaaaaaccga accgctcctt gatggcgatg acgtcgttga tggtgtggtc
     2041 gggcgagagg acgatcgggt cggcgataaa cccgttctcg tagcgcttca ccttcttcac
     2101 ctcggcggcc tgctcctcgg cggtgttgtt gtagtgtatg aatcccatcc cgcccaacag
     2161 cgccaggccg atggccatgc gcgactccgt gacggtgtcc atgggactgg ataccatcgg
     2221 cgtttttaag cgaatggacc gcgtgaggtt cgactcgagg atgacgtcct cgggcgcgaa
     2281 atcgatgtat cccggcagga tgatgaaatc gttgtacgcg agaccggtcg atgtggcgaa
     2341 cagctcgtcg gccgaaaaac cgtccagttt cttcatgatg gcgctcccgt tgcgaatgaa
     2401 tgcactgaag cgccgggggc ggacatttgt aaaggaaata attcgggaat catgctcgaa
     2461 atatacgcat ccgaacccat gccggccgtc cgtcggtcgg tttactattt tgttgcggcg
     2521 gggaaatttt tcttgcaccc ccgcgccatg cggctacaat gctatcggtc cgcacgacgc
     2581 ggaccgaccg aacatgatcc ggggtttcga atggggaggt gcaagcggcg agggttgtgc
     2641 tgccaggacg tacggctgga cgtgtccccc gagatgctgc acaacgccta cgagttctgg
     2701 cggaaatcga aatcgatcga tccgggattt tcggaaatat atttgattta tcccatgctc
     2761 gaatttaaat ttgagcacgg gagcgaggat ctcccctatc actatcggtg ccgccatttc
     2821 acgcatggag cggacgggct tcccgcctgc gccatttacg aaatacggcc ccgaatgtgc
     2881 cgggacttcc cgtattacga gagcgtggag cacctcgatc gcaccggccc cttgagcccg
     2941 tacgatgggt gcggttacaa cgatcccgac taggcggcgc gcatggcgaa tctgctttcc
     3001 gtgacggtca agttgtactc aggcatcgac aaggacctgg cgctcaccgg gtatgatccc
     3061 gccaccggaa tcggcctcac cgtcgcgcgc ggcacccggc ttcggcgcat cctgaagtcc
     3121 ctgggcatga agaaaatgtc gggaaacgcg tatttcatca acggcgcgcg ggtaggctta
     3181 tggaagagca tccgcgagga atgcgagatt gcatgcctaa agcccagcgc gggcggctag
     3241 cacgccccgc ggggggcgca tagagaggag cggaccatgg cgcgcggaat gattgtcgac
     3301 gactccaaat tcatgcggcg tatcatcaag gaaaccctcg aggaggccgg gcacgacgtg
     3361 gtggccgagg ccgaaaacgg cttcgacgcg ctcgagatgt acaagaccat gaagcccgat
     3421 ttcgtcacca tggacatcac catgtggggc aaggacggcc tcaaggcggt ctccgagatc
     3481 accgacatcg acccggacgc gcgcatcgtg gtcgtctccg cgctcagcga aaaaacgctg
     3541 cgcctcaacg aggaggggat ccgcgcgcgc gccttcctca ccaagccctt cgagaagagc
     3601 gatctcatcg gcgccatcaa caagatgctg tgaggacgtc cgcgcgcggc acatccggca
     3661 ccccatcatg agcgacacga aattcatcca gctatacgcg cagggagggc gcgccgaccg
     3721 ggcgtatttc ctcgaggacg ggagcgtgta cttcttcgcc tccgacgccg acaagtacgc
     3781 cctccgcggc cgaaacctca tcgtgggcgc gtcggagctc atcctcgcgg cgttgtgcga
     3841 caccgaaacc gggcgcctgg agaccgccgt ggccgacgag ggggcgcggc tcaagagcat
     3901 cggcgccgaa aagttcatcg cgtcgctgtc gtcctactcg ttcgcgatca acgtgtgcat
     3961 ggtgatcgcc aagcaggtgc acctcaccaa cgacatcatc aacaaaaacc ttgcggcgat
     4021 cgacggcaag gcgaaaaagg cgcgcgagtg cgcggtggag ttctaccgga tcatcgagcg
     4081 gctccgcgcc gatttcgaca agcgccgcct gccggcgctg cgggacgtga tcgtcgcgca
     4141 cgaaacgagc ctcacctaca agcgcggcga ggcgtttggg cgcgcggtcg agcagacggt
     4201 ggtcgcgccg aacgtgtcgc tcgaggagcg ctacatcgaa taccccaagg acgcgctcat
     4261 ctgcgaggag ggaacgcccg gcgacgacat gttcatcctc aagtcgggca cggtggacgt
     4321 atacgtgcag gggaaccgcg tcgcctccat cggcgagccg ggatcggtgt tcggcgagat
     4381 cgcgctgctc ttgggactcg cgcgcacggc gaccatgaag gcgaaaagcg gcgtggtcat
     4441 atcccgcatc aggaagcagg acctcaagga gatctcggcg acggacgggc ggttgctttc
     4501 ggacatcgcc gcgtcgctgg cgaagaagca ccgccacaac atcgacaaga tcgccagcgt
     4561 gaacgccatg atcctggagc gccagatcga cagcgaggcg gggaagagcg aaaaggcgcc
     4621 gctggactac aaccgcatct acaacgacct gctcgcgctc aagcgcgacg taacggcgtt
     4681 atacgaatcg aagaaggcgg acttcctcca ggacctcgtg gacggcttct aggcgccatc
     4741 ggccccgggg cccttcttga gcgacttgag ccaggcgagc tcggccttga ggtggttctc
     4801 gatatacgaa aagaggtgtt cctgcgatcc ctccgcgccg gcgcgcgaat cgccctcgct
     4861 cttcacgcgg atctccgcaa gcttctcctt gatgatgtcg atccggtcct cgatgaactg
     4921 ctcgcgctga tcgcccggga gggtgttgta gaacatgagc acgatgtcga tgtcgaagtg
     4981 aatgaggcac tgctcgtagt actgcttgag cgccttcttg aagtacttgc gcccggcggg
     5041 aaggatctga tagatatacc gctccgggtt ccccccgtcc ttttccgtcc cgaccttctt
     5101 gacccattcg tgctcgagcg ccttcttgat tgcgtaatag atggagccga acttgatatc
     5161 gacatagtcc tcgaggcgct cgaaaatctt ttttttgatt tcgtacccat agagatttcc
     5221 ctccatgagc aggccaagga tggttatttc gatcttcatt gggcgctcct cccatttgca
     5281 acgccgccat ggcgcgatcg cgcgctcacg ggccgcggga cacggacggc atggccccgc
     5341 ggagggaacc gcacgcgccc cccgcgccgc atccatcgtt cgtgccggac gcatgcgctc
     5401 gcggacggtc gcttctctat ataatgcgat agtgcgtgta gtttattttt taatatgaaa
     5461 gattgtctga ataaaaaaac aaggaatttt ttgcaaaaaa gaggaatcag gccgggggct
     5521 ttcgaagggc cgacttgata acatcttcga gagaaatgtc gggtttttcc cggacgatcc
     5581 cctcgacgag tttcgcggcg gcgctcgaat cgatgcccag cgagacaagc gcgtcgagcg
     5641 cgtcggcgct tacggtcggc gtgcgcgcgg gttcgcggga aaaggactca aggcgcgcaa
     5701 gcttgcggcg cagctcgaag atgagcttct ccgctttcgc cttgcccacg cccggaaccg
     5761 acgtgagcat ggccggctcg ccgtcgcgca ccgcggcgac cagcgcctca ccgtcgatgc
     5821 tcgatatgat tgacagcgcg agcgccggac cgatgccggc cacgttcagg aggatcgcga
     5881 agagctcccg ctcgcgcctg gtgaagaatc cgaacaggcg gaactggtcc tcgcggtgga
     5941 gcgtgtgcac gtagaggagc gcgctgggcg cgtcgcgcag cgcctcgtag gttgacagcg
     6001 gtatggcgca ctgatacccc acgccgttga cgttgatgac gacgtcggtg ggcctgaggt
     6061 cctcaaccgt tcccgtaagc gttgcgatca tgacaccgta tcccattgtg ggcgatgcaa
     6121 tgacacaggg cgatggcaat ggcgtccgcg gcgtcgtccg gccggggaag ctcgcggagc
     6181 cgcaggatct tcattaccat ggtttgcatc tgccgtttgg tggcgcgccc gtagccggtg
     6241 atcgactgct tcacctgcat cgggttgtac tccgcgattt cgagcccggc caggcgcgcc
     6301 gtgagcagcg ccgccccgat gcacttggcc acgtccatcg cggtggtggt attgcgcgag
     6361 aagaacaacc gctcgatggc gcagcagtcc ggacggtgct cgtcgatgat gcgcgcgagc
     6421 ccgtcatgga taatttgcag ccggccgccg atgccgtccc tcccgtccgg ggtgatcacc
     6481 ccataggcga tcaggtgaaa gttcgattcg aggagcgacc accccagaat gctcgatccg
     6541 ggatcgatgc cgagcacgcg cacctaggcc tcgccgagcg ccatcatcac ctcgtcgggg
     6601 atgtcgtagt tcgagtacac gttctggaca tcgtcgtggt cgtccagaag gtcgatgagc
     6661 cgcaggcact gctgcgcggt cttctcgttg aggctcacgg tgctcttggg gaggaacgtg
     6721 atctcgttat aggcggtcgc gagccccttg ttcttgatgg cgctgtaaac gtcgttatac
     6781 gcctccggcg acgtggtcac cacgatcacc ttgtcctcgg tgcgaatgtc gtgaatgtcg
     6841 aaatccagga gaagctccat gaggttgtcc tcggcgatcg cgccggcgtc cacatggacc
     6901 acccccttac gctcgaaatt gtaccccacg cagccggtct ctcccaggtt cccgccgttt
     6961 ttgctgaaga tggtgcggat ctcgggcgtg gtgcggttcc ggttgtcggt gagacagtcc
     7021 accatgatcg cgacgccgcc gggcccgtag ccctcgtagc ggat
//
LOCUS       DAORHF010000195         7015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10538_length_7015_cov_6.456322, whole genome shotgun sequence.
ACCESSION   DAORHF010000195 DAORHF010000000
VERSION     DAORHF010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7015
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10538_length_7015_cov_6.456322"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="PKJ16_14055"
     CDS             <1..1506
                     /locus_tag="PKJ16_14055"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV48164.1"
                     /translation="MVLLDLQEGLGELEIKKHHRLAYRSLSAAKIYLALSIITPLFLS
                     FAVCVHIQIVSAVIGLVLAIAVGVYAQVQGYRPAKFFLVAWTASLVGGIIYALKVLAV
                     LPANFVTTYALQFGTVLQFILLALGMGDRINLMKQEKEHAQAEALMGHELATEMFKES
                     AKLSEEHLAAMERKNVEIEALNRDLEKKVDDLNEANRRITLSEERYRLLIEGTNDIIF
                     SLDERWNFTSINRAVLAHFKVKPEAVIGRNILELMYEGPGGNTTRQLVIEKLEEFARE
                     RRPISFRAEFISPIISEPREMQVRLEYLSIAGRVEVLGKASSVAEDVLLKYFEYEKQR
                     YSIGNFLITSEEISHRITRNLYRYFDHREITLIRIALREIIINAIEHGNLEISFEDKT
                     AAMQEERYFDLVAERQRHPRFSSRRVHIEYAIDPEKIAYKITDEGPGFDYHAVLNADP
                     DAVNENMLSHGRGITMAREVFDSIEYNKKGNQVLLVKNAFTAALEDERIEF"
     gene            1522..2412
                     /locus_tag="PKJ16_14060"
     CDS             1522..2412
                     /locus_tag="PKJ16_14060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HNV48165.1"
                     /translation="MKESSSTTAVDTLASRAIASIAPRRRRILHDPFARSLLPAHWLV
                     PKFYFWQARFNPLVYPISVLIADLVSPGGVLQIALRHRHMDECLARAIRDGVRRIVIL
                     GAGYDSRCMRFRTDGVEFIEIDHPHTQNAKHRRLDRINGGPVPGVRYLPIDFCGDWAA
                     DVAREIGATQHPAFFIWEGVAYYLPEEAVRYTLAAIRRCSPAGGRIVFDMFPRGIADP
                     ACADPVLRKMRRYGERHREHFQWGCDRSEMPGFLAECGLRLDSTITLREYADRLRADE
                     GIRIGDAAVLARLYNVEASW"
     gene            complement(2432..3157)
                     /locus_tag="PKJ16_14065"
     CDS             complement(2432..3157)
                     /locus_tag="PKJ16_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HNV48166.1"
                     /translation="MRTIAKRMAVLCAITLAALIACSTPGVKEAPGTYLNPAAIDVSA
                     VLPAPPADGSLAGLADLETVRMVQSWRTPDQIAWAKKTDGEDPFHYNAVVGSWFTAKN
                     LPGCAKFFMQVLHDARKVSNRVKDLHKRPRPPKRDERVTPSLPIPKSLSYPSGHSTRT
                     FTIAYVIAELFPDKRTAVVDFAHRDAWARIIAGVHYPTDDVGGRLLAGAVFSELNKSA
                     KFRATLDECRAEIAAYRKRAGVR"
     gene            complement(3205..3606)
                     /locus_tag="PKJ16_14070"
     CDS             complement(3205..3606)
                     /locus_tag="PKJ16_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48167.1"
                     /translation="MDISTVLPYYIVTVMVLALGSMLFFFRRKERRHPVEQGKTPLYS
                     ETCGGMIGMFRYTVPFLRLSVYEDQIVIGAFKPIALALGDIAKIEIGRFLLSTGIRIH
                     HRRTDVPAKIVIWTGAAEKVRSLLAGKVAFE"
     gene            3872..>7015
                     /locus_tag="PKJ16_14075"
     CDS             3872..>7015
                     /locus_tag="PKJ16_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48168.1"
                     /translation="MDTSPVAHLSERAPIDPEALRIPVAATAGALSRVSGLIEELKHP
                     NTDWKAAVDGLRAYLYDYLYEIAPHARSVLPTVFHFLREATARRKASTLRAADTVFDR
                     YAFAIRRAADGDDALAPVAAAFDEWCPAYLDLLIGESDDNFYYGPVNARAMALRELIL
                     RTGRHADGLPGRVGEFLLRQYGIFISHAIRTTRAEIDEIVGLLDGTEESRQFALLLEA
                     VSEEAYADRLSVLAARLRRDPTAALSAGDGAPDFSHNARTWERICLSARRAIEEGRVS
                     GDDAVLRLIAFITRKSAEGRDAELQRFMSRTAATICAFLVDNRRLELIRSVIDLVMPP
                     LLAEAERGAGFYSAFSTITMIGETIIASGITPLVDYFVDILVHSKFCFPEFRGIAADW
                     SVTVNASHLENIRTWMRLIELNPALMKKLAASLIVNLKLGGVFLKDTDVFQRDISRLL
                     RSDYREAFHLITSLAAVFPAFYHDIGATGDIRAFTERIDTNHEMNDLIHFIRKQVHVE
                     SSSRTVHLVQRVMEFWMTGDRSLLSGLVPDEVYRELDGFYRMVNLDREGPARAIVDAA
                     RRAFPEHAALPFWDFLLAVGRERFTSFVAEGEIAGVSRADRDDAAACFASYFETRNPA
                     EMTVMLELIRNKFGVDVSKQAIWAFLYEISDEEFRDLFGGTDTSAISSVNIEKFITFL
                     HVYRMLYDKYNFSDVRCAQKLRHYADEELFEAPEGFFELLDGADTIAALEALLALQHT
                     LKNDILLGEKTYEPVDTIEFKRHIAFGIPSMYGSYKEKKFDTLKVFFHLNLVRLRLFE
                     TVIERLADLPTGIDYPTVKTVLTLFFKTFIIDGLANQEMVMVSDLLSSPHITVAQFRD
                     IVTNLHNIHGEIADQFNETFTDVCRQAIGSIGIDNIAAKYIPRENPQSIDVIIDRFLR
                     DQIMQSPLLQLMDNLLIRLKDYLTEELYNRGDFVCLNGSQPRFSRGRNLFPLRVAAHQ
                     PPEGTTHAPVWKVGAKGHGLVFAANMDGIAVPEGFVLSTELFKRIRDGSVNNPRFRRK
                     VIYSLRRYVDAFTGGRFA"
BASE COUNT         1296 a         2330 c         2139 g         1250 t
ORIGIN      
        1 atggtgcttc ttgatctcca ggaaggattg ggtgaactgg agatcaagaa gcaccatcgg
       61 ctggcatacc gttcactgag cgccgccaag atctatctgg cgctctccat catcaccccg
      121 ctgtttttga gctttgccgt gtgcgtgcat atccagatcg tctccgccgt catcgggctc
      181 gtcctcgcga tcgcggtggg agtgtacgcg caggtccagg gataccggcc ggcgaaattc
      241 ttcctggtcg cgtggacggc ctcgctggtg ggcggcatca tctacgcgct gaaggtcctg
      301 gcggtgctgc cggcgaactt cgtcaccacc tacgccctgc agttcggcac cgtgctgcag
      361 ttcattctcc tggccctggg catgggcgac cgcatcaacc tgatgaagca ggagaaagag
      421 catgcccagg ccgaggcgct catgggccac gagctcgcca ccgagatgtt caaggaatcg
      481 gcgaagctct ccgaggagca tctcgccgcc atggagcgca agaacgtcga gatcgaggcc
      541 ctcaaccgcg acctcgaaaa aaaggtggac gatctcaacg aggccaaccg gcggatcacg
      601 ctctcggagg agcgctaccg gctgctcatc gaggggacca acgacatcat cttctcgctg
      661 gacgagcgct ggaatttcac cagcatcaac cgcgcggtgc tggcgcactt caaggtgaag
      721 cccgaggcgg tgatcgggcg gaacatcctc gagctcatgt acgaggggcc gggcggcaac
      781 accacgcgcc agctcgtaat cgagaaactc gaggagttcg cccgcgagcg gcgtcccatc
      841 tcgtttcgcg cggagttcat atcgcccatc atctccgagc cgcgcgagat gcaggtgcgc
      901 ctggagtacc tgtccatcgc ggggcgcgtc gaggtgctgg gaaaggcgtc cagcgtggcc
      961 gaggacgtcc tgctcaaata cttcgagtac gagaagcagc gctacagcat cggcaacttc
     1021 ctgatcacct cggaggagat ctcgcaccgg atcacgcgca acctctaccg ctacttcgac
     1081 caccgcgaga tcacgctgat ccgcatcgcg ctgcgggaga tcatcatcaa cgccatcgag
     1141 cacggcaacc tggagatcag cttcgaggac aagaccgcgg cgatgcagga ggagcgctac
     1201 ttcgacctgg tggccgagcg ccagcgccat ccccgcttca gctcccgccg cgtgcacatc
     1261 gagtacgcca tcgacccgga gaagatcgcc tacaagataa ccgacgaggg cccggggttc
     1321 gactaccacg cggtcctgaa cgccgacccc gacgcggtga acgagaacat gctctcgcac
     1381 ggccgcggca tcacgatggc gcgcgaggtg ttcgattcga tcgagtacaa taaaaagggc
     1441 aaccaggtgc tgctcgtcaa gaacgcgttc accgccgccc tcgaagacga acgcatcgag
     1501 ttctaacgac gggcatcgac aatgaaggaa tcatccagca cgaccgccgt agacacgctc
     1561 gcctcgcgcg ccattgcgag catcgcgccg cgacggcggc ggatcctgca cgatcccttc
     1621 gcccggtccc tgctgccggc gcactggctt gtcccgaagt tctacttctg gcaggcccga
     1681 ttcaatccgc tggtttatcc gatcagcgtc ctgatcgccg atctggtgag ccccggcggc
     1741 gtgttgcaga tcgcgctccg gcaccggcac atggacgagt gcctcgcgcg ggcgattcgg
     1801 gacggcgttc gccgtatcgt catcctcggg gccgggtacg attcgcgctg catgcggttc
     1861 cgcaccgacg gcgtcgagtt catcgagatc gaccacccgc acacccagaa cgcgaaacac
     1921 cggcgcctcg accggatcaa cggcggaccg gtcccgggcg tccgctacct gccaatcgat
     1981 ttctgcggcg actgggcggc ggatgtcgcg cgcgagatcg gcgcaacgca gcacccggcc
     2041 ttcttcatct gggagggcgt cgcctattac ctgcccgagg aggcggtgcg ctacaccctt
     2101 gccgccatcc ggcgctgttc acccgcgggc ggccggatcg tcttcgacat gttcccgcgc
     2161 ggcatcgccg acccggcctg cgcggacccc gtgctgcgca aaatgcggcg atacggggag
     2221 cgccatcgcg agcatttcca gtggggatgc gatcggagcg agatgccggg cttcctcgcc
     2281 gagtgcggcc tgcgcctgga ttcgacgatc accctgcggg aatatgccga ccggctgcgg
     2341 gccgacgagg ggatccgcat cggggacgcg gcggtgcttg cacgcctcta caatgtcgag
     2401 gcgtcgtggt agcgggcgcg tcggaccggc gctagcgaac gccggccctc ttccggtacg
     2461 ccgcgatctc cgcccggcac tcgtccagcg ttgcgcgaaa tttagcgctc ttgtttaact
     2521 cggaaaatac ggccccggcg aggaggcggc cgccgacatc gtcggtggga tagtgcacgc
     2581 cggcgataat ccgcgcccag gcatcgcggt gggcgaaatc cacgacggcc gtccgcttgt
     2641 cgggaaacaa ctcggcgata acgtaggcga tcgtaaaggt gcgcgtcgag tgaccgctgg
     2701 gatacgagag gctcttcgga atcggcaggc tcggggttac gcgctcgtcc cgtttcggcg
     2761 ggcgcgggcg cttatgcagg tccttcacgc gattgctcac cttccgggcg tcgtgcagca
     2821 cctgcataaa aaacttcgcg cagccgggaa ggttcttcgc cgtaaaccac gatcccacga
     2881 ccgcattata atggaacggg tcctcgccgt cggtcttttt cgcccaggcg atctgatccg
     2941 gcgtgcgcca ggactgcacc atgcgcaccg tctccagatc ggccagaccc gcaagcgacc
     3001 catcggccgg gggcgccggt agtacggctg acacgtcaat ggccgcgggg ttcaggtagg
     3061 tgcccggcgc ctccttcacg ccgggcgtcg agcaggcaat aagcgcggcg agggtgattg
     3121 cgcacagcac ggccattctc ttcgcgatcg ttctcatcaa cagttcctcc ctgcaggcag
     3181 tgggttcgtt ccggcggcgc atcatcattc gaacgcgacc tttcccgcga gcagcgaccg
     3241 caccttttcg gccgccccgg tccagatgac gatcttcgcg gggacgtcgg tccgccggtg
     3301 gtggatgcgg atccccgtcg agagcaggaa ccgtccgatt tcaatcttcg cgatatcgcc
     3361 caacgcgagc gcgatcggct tgaacgcgcc aatcacgatc tggtcctcgt agacgctcaa
     3421 tcggagaaag ggaacggtgt atcggaacat gccgatcatg cccccgcacg tttcgctgta
     3481 caacggcgtt ttgccctgct ccacggggtg gcgccgttcc ttgcgccgga agaaaaacag
     3541 catcgagcca agggccaaca ccataacggt gacgatgtaa tacggcaaca ctgtcgagat
     3601 atccataata acaacgcccc tcatcggtgc gatttcacta ccatacccta tgcatacgca
     3661 ggcatagcgc gcaatcattt tttctgaaca caagaaagac gatacacaaa attggatttt
     3721 ttgttcacct ggggcataag gattgacaaa aaccacaccg aagaaaagat ggactcaaga
     3781 gggacgcaac aaccggttgt acgctgatcg actttatgca gaccccatcc gcgtcaggga
     3841 gcgggcgatg tatatgcgga ggccggcata catggatacc tcaccggttg cacatctttc
     3901 cgagcgcgcg ccgatagacc ccgaggcgtt gcgcattccc gttgcagcca cggcaggcgc
     3961 gctgtcacgc gtgtccggcc tcatcgaaga actcaaacac cctaataccg actggaaggc
     4021 ggccgtggac ggcctgcgcg cctacctgta cgactatctc tacgaaatcg ccccgcacgc
     4081 gcgctcggtg ctgccgacgg tctttcattt cctgcgcgag gcgaccgcgc gacgaaaggc
     4141 ctcgaccctg cgcgcggcgg acaccgtgtt cgaccgctac gccttcgcta tccgacgggc
     4201 cgcggacggt gacgacgcgc tcgcgccggt cgcagcggca ttcgacgagt ggtgcccggc
     4261 gtacctcgac ctgctcatcg gcgagagcga cgacaacttc tactatgggc ccgtgaacgc
     4321 gcgcgcgatg gcccttcgcg aactcatcct ccggaccggc cgtcacgccg acggcctgcc
     4381 gggccgcgtc ggtgaattcc tgttgcggca gtatggcatc ttcatctcgc atgcgatccg
     4441 caccacgcga gcagagatcg acgaaatcgt cggcctgctc gacgggaccg aggaatcgcg
     4501 ccagttcgcg ctcctgctcg aggcggtttc cgaggaggca tacgctgacc ggctgtccgt
     4561 gctcgccgcg cgattgcggc gcgatccgac cgcggcgctc tcggccggcg acggcgcgcc
     4621 cgatttttcg cacaatgccc gcacctggga gcgcatctgt ctgtccgcgc ggcgcgcgat
     4681 cgaggaaggg cgcgtctccg gcgacgatgc ggtgctgcgc ctcatcgcct tcatcacgcg
     4741 caagtccgcc gaggggcgcg acgccgagct ccagcgcttc atgtcccgca cggcggccac
     4801 gatctgcgcc ttcctggtcg acaatcgacg tctcgagctc atccgcagcg tcatcgacct
     4861 ggtcatgccc ccgctcctgg ccgaggcgga acggggcgcg ggcttctact cggccttctc
     4921 caccatcacc atgatcggcg agaccatcat cgcctcgggc atcacgccgc tcgtggacta
     4981 cttcgtggac atcctcgtgc actcgaagtt ctgcttcccg gaattccgcg ggatcgccgc
     5041 cgactggtcg gtgacggtga acgcgagcca cctggagaac atccggacct ggatgcggct
     5101 catcgagctc aatcccgcgc tcatgaaaaa gctcgcggcg agcctcatcg tgaacctcaa
     5161 gctgggcggg gttttcctga aggacacgga cgtctttcag cgcgacatct cgcgccttct
     5221 gcgcagcgat taccgcgagg cgtttcacct catcacctcg ctcgcggccg tgtttcccgc
     5281 gttctaccac gacatcggcg cgaccggcga catccgcgcc tttaccgagc gcatcgacac
     5341 caatcacgag atgaacgacc tcatccactt catccgcaag caggtgcacg tggagtcgag
     5401 cagccgtacg gtgcacctcg tccagcgggt gatggagttc tggatgacgg gcgatcgctc
     5461 gctcctgtcg ggactggtcc cggacgaggt gtaccgggag ctcgacggat tctaccgcat
     5521 ggtgaatctg gatcgcgagg gaccggcgcg cgcgatcgtc gatgcggccc ggcgcgcatt
     5581 cccggagcac gccgcgctcc ccttctggga tttcctgctc gcggtgggcc gcgagcgttt
     5641 cacgtccttc gtcgccgagg gcgagatcgc cggagtgagc cgcgccgatc gtgacgacgc
     5701 ggcggcgtgc tttgcgagct acttcgaaac gcgcaatccc gcggagatga ccgtcatgct
     5761 ggagctcatc cgcaataagt tcggcgtgga cgtgtcgaag caggcgatct gggcctttct
     5821 ctacgagatc tccgacgagg aattccgcga cctcttcggc gggaccgaca ccagcgccat
     5881 ctcctcggtg aacatcgaga agttcatcac cttcctgcac gtgtaccgga tgctgtacga
     5941 caaatacaac ttctccgacg ttcgctgcgc gcagaaactg cgccactacg ccgacgagga
     6001 gctcttcgag gcgcccgagg gcttcttcga gctcctcgat ggcgccgaca ccatcgccgc
     6061 gctcgaggcg ctgttggcgc tgcagcacac gctcaagaac gatattctgc tgggggagaa
     6121 gacctacgag ccggtggaca cgatcgagtt caagcggcac atcgccttcg gcatcccctc
     6181 gatgtacggc tcgtacaaag agaagaagtt cgacacgctc aaggtgttct tccatctcaa
     6241 tttggtgcgc ctgcggctct tcgagaccgt catcgagcgc ctggcggacc tgccgacggg
     6301 catcgactac cccacggtca agacggtgct cacgctgttt ttcaagacct tcatcatcga
     6361 cggcctggcc aaccaggaga tggtgatggt gagcgacctc ctctcgtcgc cgcacatcac
     6421 cgtcgcgcag ttccgcgaca tcgtgaccaa tcttcataat attcacggcg agatcgccga
     6481 ccagttcaac gagaccttca ccgacgtgtg ccgccaggcg atcgggagca tcgggatcga
     6541 caacatcgcg gccaagtaca tcccccgcga aaacccgcag agcatcgacg tgatcatcga
     6601 ccgctttttg cgcgaccaga tcatgcagtc gcccctgctg cagctcatgg acaacctcct
     6661 catccgcctc aaggactatc tgaccgagga gctgtataac cggggcgact tcgtgtgcct
     6721 caacggctcg cagccccgct tcagccgggg gcgcaacctc ttcccgctcc gcgtcgccgc
     6781 gcatcagccc cccgagggga cgacccacgc gccggtgtgg aaggtgggcg ccaagggaca
     6841 cggtctcgtg ttcgcggcga acatggacgg gatcgcggtg cccgaggggt ttgtcctttc
     6901 cactgagctg ttcaagcgca tccgggacgg ctccgtgaac aacccgcgct ttcgccgcaa
     6961 ggttatctac tcgctgcgcc gctacgtcga tgcgttcacg gggggacggt tcgcg
//
LOCUS       DAORHF010000196         7000 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10573_length_7000_cov_5.825918, whole genome shotgun sequence.
ACCESSION   DAORHF010000196 DAORHF010000000
VERSION     DAORHF010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10573_length_7000_cov_5.825918"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="PKJ16_14080"
     CDS             complement(<1..1908)
                     /locus_tag="PKJ16_14080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HNV48169.1"
                     /translation="MDEIFGPRGVLCEVYPSFEHREEQAQMAAFVAERMHERENALIE
                     AGTGVGKTLAYLIPALVYCRENKKTLAVSTETRALQKQLADKDIPIAKAALARLFGWE
                     PTVELCLGSANYPCLRRFELMVAKGSFAEGDVDAVRELRERFAKGDVFTRFDVRVRGA
                     LWREFMREPDACSFANCPFFGRCIFQRARRAWAEADCLIMNHYLFFSNVASGRAYLPE
                     FEVAVFDEAHSLEGIACDQLGFTLEYHTFVEDLGRLHVKGRKRSLIAAIPRDEVRRAL
                     IRKIGAIAAGGSAFFESIRERHLASKTSQRIREPLAFGADLARDLTSFIADAQTAIGL
                     LEDDHQRLEFEIALGRLLVHAQNLNAAVYHDRANYVYWAERRDDELLGAVSLRGQPIE
                     VGDIMAEEVNGFYESSLYFSATLAVGGDFSFMEERLGLHRNRNLLLGSPFDYARQVVL
                     YVNADGSDPGDRSYREDAARTAADIIEHLGGNCLLLFTSYAMLEEVRDMIAGMVSAPL
                     YAQGEYPANEVLERYVADEGSVLLGTHSFWQGIDLPGDLLRGVIMFRLPFFVPDRPEV
                     QARMERMEAAGQNPFSHYQVPHAVIRFRQGFGRLIRGHADRGVVAVLDPRIVTKGYGR
                     QFLRSIPACRVV"
     gene            2134..2544
                     /locus_tag="PKJ16_14085"
     CDS             2134..2544
                     /locus_tag="PKJ16_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48170.1"
                     /translation="MNANRNDDTAADGLNSAHTERFYTITLRDNHALFEINLGNFDFY
                     ETPKMLSLIEASLESLKHPRTVIDLKKVAIIDSAGIGSLIAIKNILERNGSELVLLNE
                     SEHIMRILEITKMKGFFTVFRSPDEVRGYLDGKK"
     gene            complement(2549..3034)
                     /gene="rfaE2"
                     /locus_tag="PKJ16_14090"
     CDS             complement(2549..3034)
                     /gene="rfaE2"
                     /locus_tag="PKJ16_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HNV48171.1"
                     /translation="MRVRDVREKIVDGDAVRALADDIRASGRTIATTNGCFDIVHRGH
                     VEYLQEAARHADVLIVGVNSDESVRRLKGPTRPVNDERSRALVLAALEFVDYCVIFGE
                     QTPVELLGRIRPHVHVKGGDYRPDDLPEKVVVEAGGGRIVIVGLVDGFSTTGIIGRMS
                     G"
     gene            complement(3063..5168)
                     /locus_tag="PKJ16_14095"
     CDS             complement(3063..5168)
                     /locus_tag="PKJ16_14095"
                     /inference="COORDINATES: protein motif:HMM:NF025691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HNV48172.1"
                     /translation="MIIRCTHCNSAFAVDDSKVENRKFAFTCPKCATSNIIDNRMAEK
                     TPAAAGARHADRFGDEAPARDAKTGSHDPGDDLLDASFDEDATDGRDAAPKRKAVDDE
                     LDLGEFDIPDEDAPGEPSGAAGGAKKPSRPIAQDDEIDLDQLGLSEDEELSALINEDV
                     APGDDAPATKKPRGGDDIASELDDLESALRHEETLQDLEPLEEDLSFDEPKAGGAKGG
                     GAKAGGAAAGIDVDLDGLLDDDTLGASSKEDEVFAKDMGDTDESITIDLDTLDIQLEE
                     DAGVKKPSAMADDIDALPELTDAELGELSGAGGAAGEEDENITIDLDSLDLPLEDEGE
                     LKQGEIIDEDETITIDDAGLTMDDLEVGAPTGKSARHAHDDLDAHDDDIKLSLDEIDP
                     TLDVNELGGAPAEGERLLTEEIDGEDLPEIDFDKFESEEPGPGRTAAIAAGAGIVGTA
                     AGLGITAASTGRGSKQRADLGADDFLDIERQEEYARYQDDLDRDDRRPSDTVPRGAVN
                     FSIDYSLGFTRLGAVLRLFGLYYIRLIPHAVVTFLYIALAGMVGFINWLIILFTGESS
                     EDFTSIQEKAVRASLSLSACAADVVEDVPAFAGVKDIDYPLQFEVTYPVAYSKALAFL
                     RLTGIGIILATLPHLIILFVLTVGAQLIGLVGLVAIIATRRWPNILFDFMIRFYRYQS
                     NVLSFLFGLVDKYPTFRFE"
     gene            complement(5187..5495)
                     /locus_tag="PKJ16_14100"
     CDS             complement(5187..5495)
                     /locus_tag="PKJ16_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48173.1"
                     /translation="MKNEYAPENNLVILFLLLVFTAGLYYFWWLARTSRMFNDNPVSN
                     VLLVVLTFGVWSLYINLKYMQKAEELNGRGFGWYMVFFLVISPLVIQHNINERFFPGR
                     "
     gene            5756..>7000
                     /locus_tag="PKJ16_14105"
     CDS             5756..>7000
                     /locus_tag="PKJ16_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48174.1"
                     /translation="MASATYFHYTDTGMRRRLAVAIAAIMVVLGFFCADICASLHAYY
                     PYHRALTLGLLGALGCLLAGLAIGSRFYAIGGRRGPFRILAVLIAAITAAGIIATVLT
                     APERDLRAELLLISSFFLVPLVGLLPFLAGMYIAYPLKLACGDFIDGQSALAPFVLAL
                     FAGLAAGVAMFVASCYVDALRVLPLVAIAASAPFVFAINLDFSPSAVTTDESAPPPQS
                     SHFPGPDALERAITLTRYAVPLALAVLGYVSLQRFFGDTFHISAAIIGFALAAVPAGF
                     AAAHLLRRLPWRNFVEPLAPIAFLLPLYAQALYAHISFTAATLLYAPVMIIFGFSLYS
                     ALESLRAQYAHGRRFGVVIAYAAVVPIALFALAAFAPLVNAAYVAIVACASLMLVAAA
                     TAAGLSRATTVHQRIAHAALAPA"
BASE COUNT         1203 a         2411 c         2132 g         1254 t
ORIGIN      
        1 gaccacccga catgcgggaa tggacctgag gaactgccgc ccgtagccct tggtcacgat
       61 gcgcggatcg agcacggcga cgaccccgcg atcggcatgg ccgcggatga ggcgcccgaa
      121 gccctgccgg aagcgtatca cggcgtgtgg gacctggtag tgcgaaaagg gattctggcc
      181 cgccgcctcc atccgctcca tgcgcgcctg gacctccggc cgatccggaa cgaagaacgg
      241 caggcggaac atgatcaccc cgcgcaggag gtcgcccggc aggtcgatcc cctgccagaa
      301 ggagtgcgtc cccaggagca ccgagccctc gtcggccaca tagcgctcga ggacctcgtt
      361 cgccggatac tcgccctgag cgtacagggg cgccgacacc atcccggcga tcatgtcgcg
      421 cacctcctcg agcatcgcgt acgaggtgaa caacagcagg cagttcccgc caaggtgttc
      481 gatgatatcg gccgcggtcc gggcggcgtc ctcgcggtac gaccggtcgc cgggatcgga
      541 gccgtcggcg ttcacgtaca gcaccacctg gcgcgcgtag tcgaaggggg agcccagcag
      601 cagatttcgg ttccggtgca ggcccagccg ctcttccatg aacgagaagt cgccgcccac
      661 cgcgagggtg gcggaaaaat acaggctgga ctcgtagaag ccgttcacct cctcggccat
      721 gatgtcgccc acctcgatgg gctggccgcg caggctcacg gccccgagga gctcgtcgtc
      781 gcgccgctcg gcccagtaaa cgtagttcgc gcggtcgtga tacaccgcgg cgttcaggtt
      841 ctgcgcgtgg accaggaggc gccccagcgc gatctcgaac tcgagccgct gatggtcgtc
      901 ctccaggagc ccgatggccg tctgcgcgtc ggcgatgaac gacgtgaggt cgcgcgccag
      961 atcggcgccg aaggcgagcg gctcgcggat gcgctgggac gtcttcgacg ccaggtgccg
     1021 ctcccggatc gactcgaaaa acgccgatcc gcccgcggcg atcgcgccga tcttccggat
     1081 gagcgcgcgc cgcacctcgt cgcgcgggat ggccgcgatc agcgagcgct tgcggccctt
     1141 cacgtgcagc cgccccaggt cctcgacaaa ggtgtgatac tcgagcgtga agccgagctg
     1201 gtcgcaggcg atgccctcca gcgaatgcgc ctcgtcgaat acggcgacct cgaactccgg
     1261 cagataggcc cgaccggagg cgacgttcga aaagaagaga tagtggttca tgatgaggca
     1321 gtccgcctcg gcccaggcgc ggcgcgcgcg ctggaagatg caccggccga agaaggggca
     1381 gttggcgaag gagcacgcat cgggctcgcg catgaattcg cgccacagcg cgccgcgcac
     1441 gcggacatcg aaacgcgtga agacgtcgcc cttcgcgaac cgctcgcgca gctcgcggac
     1501 cgcgtcaaca tcaccctccg cgaacgagcc cttcgccacc atgagctcga agcgccgcag
     1561 gcagggataa ttggcgctcc ccaggcagag ctcgacggtg ggctcccatc cgaagagccg
     1621 cgcgagcgcc gccttcgcga tggggatgtc cttgtcggcg agctgtttct gcagggcgcg
     1681 cgtttcggtg ctcacggcca gcgtcttctt gttctcgcgg cagtagacga gggcggggat
     1741 gagataggcg agcgttttcc ccaccccggt gcccgcctcg atgagcgcgt tctcccgttc
     1801 gtgcatccgc tccgcgacga aggccgccat ttgcgcctgc tcctcccggt gctcgaacga
     1861 cggatacacc tcgcacagga cgccgcgcgg gccgaagatt tcatccatga cgggccgtcc
     1921 cctggggcgg gtttgttcta tcaactctgt ccattcgttg tccgttcgaa gacggacctt
     1981 tccaacaggc ggcctccctg tccataacaa tatttccggt accggcggcc gcgaaaaagc
     2041 gcttgataaa aacggccccc catgcacact ctccccgcga tgttcacctg cgccggcgca
     2101 gtgaacacaa ccggcaccac acgagcgtca gccatgaacg ccaaccgcaa cgacgacacg
     2161 gccgcagacg gcctcaacag cgcccacacg gagcgcttct acaccatcac gctgcgggac
     2221 aatcacgccc tcttcgagat caacctgggc aatttcgatt tctacgagac gccgaagatg
     2281 ctcagcctca tcgaggcgtc gctcgagtcg ctcaagcacc cgcgcaccgt catcgacctc
     2341 aagaaggtcg ccatcatcga ctcggcgggc atcggctcgc tcatcgccat caagaacatc
     2401 ctggagcgaa acggcagcga actggtcctc ctcaacgaga gcgagcacat catgcgcatc
     2461 ctcgagatca ccaagatgaa ggggtttttt acggtgttcc gctcgcccga cgaggtgcgg
     2521 ggatacctgg acgggaaaaa gtagtggcct agccggacat cctaccgatg atgccggtgg
     2581 tggagaaccc gtccaccagg ccgacgatga cgatcctgcc gccgcccgcc tcgaccacaa
     2641 ccttctccgg aagatcgtcg ggacggtaat cgccgccctt cacgtgcaca tgcgggcgga
     2701 tcctcccgag gagctcgacg ggcgtctgct ccccgaaaat cacgcaatag tcgacgaact
     2761 ccagcgcggc cagcaccagc gcgcggctgc gctcgtcgtt caccggacgc gtggggccct
     2821 tgaggcgccg cacggattcg tcgctgttta ctcccacgat gaggacatcg gcgtgacgcg
     2881 cggcctcctg gaggtactcc acgtggccgc ggtgtacaat atcgaaacag ccgttggtgg
     2941 tcgcgatggt gcggcccgat gcccggatgt cgtccgccag cgcgcggacc gcgtcgccgt
     3001 cgacgatctt ttcgcgcacg tcgcgcactc tcatggccgc aggtctcccg ggtccgttcc
     3061 gcttactcga accggaaggt ggggtatttg tccaccaggc cgaagaggaa cgagaggacg
     3121 ttcgactggt atcggtagaa gcggatcatg aaatcgaaga ggatattggg ccagcgccgc
     3181 gtggcgatga tcgcgacaag ccccacgaga ccgatgagct gcgccccgac cgtgagcacg
     3241 aacaggataa tcaggtgcgg cagcgtcgcc agtataattc cgatcccggt gagccgcaaa
     3301 aacgccagcg ccttcgagta ggccaccggg taggtgacct cgaactggag cggatagtcg
     3361 atgtccttca cgccggcgaa ggccggcacg tcctcaacca cgtcggccgc gcaggcgctc
     3421 aacgacagcg aggcgcgcac ggccttttcc tggatcgagg tgaagtcctc cgacgactcg
     3481 ccggtgaaca ggatgatgag ccagttgatg aagccgacca tgccggccag cgcaatgtac
     3541 aggaaggtca cgaccgcatg cgggatcagc cggatataat agagcccgaa cagccgcagc
     3601 accgcgccca gccgggtgaa gcccagcgaa taatcgatcg agaaattcac cgccccgcgg
     3661 gggaccgtat cggacggccg tcgatcgtcc cgatcgagat cgtcctgata gcgggcatac
     3721 tcctcctggc gctcgatgtc gaggaaatcg tcggcgccaa gatcggcgcg ttgtttcgat
     3781 ccgcggcccg tggacgccgc cgtgatcccg aggcccgccg cggttccgac gatccccgcc
     3841 ccggccgcaa tggcggcggt ccggcccggg cccggctcct cgctctcgaa tttatcgaag
     3901 tcgatctcgg gcaagtcctc gccgtcgatc tcctcggtaa gaagccgctc gccctccgcg
     3961 ggcgcgccgc cgagctcgtt cacgtccagg gtgggatcga tctcgtcaag gctcagtttg
     4021 atatcgtcgt catgcgcgtc gaggtcgtca tgcgcgtgac gcgccgactt ccccgtaggc
     4081 gcgccgacct cgagatcgtc catcgtgaga ccggcgtcgt cgatcgtgat cgtctcgtcc
     4141 tcgtcgatga tctcgccctg cttcagctcg ccctcgtcct ccaggggcag gtcgagcgaa
     4201 tcgaggtcaa tggtgatgtt ttcgtcctcc tcgcccgccg cgccgccggc cccggacagc
     4261 tcgcccagtt ccgcgtcggt gagctcgggg agcgcgtcga tgtcgtcggc catcgccgac
     4321 ggcttcttca cgcccgcgtc ctcctcgagc tgtatgtcca gcgtatcgag gtcaatggtg
     4381 atgctctcgt cggtgtcgcc catgtccttg gcgaagacct cgtcctcctt cgacgatgcg
     4441 cccagcgtat catcgtccaa caaaccgtcg agatcgacgt cgatcccggc ggccgcaccg
     4501 ccggccttcg caccgccgcc cttcgccccg cccgccttcg gttcgtcgaa ggacaggtcc
     4561 tcctcgagcg gctcgaggtc ttggagggtc tcctcgtggc gcagggcgct ttcgaggtcg
     4621 tcgagctcgc ttgcgatatc gtctccgccg cgcggcttct tcgtcgcggg cgcgtcatcg
     4681 ccaggggcga cgtcctcatt gatgagcgcg gagagctcct cgtcctcgga aagcccgagc
     4741 tggtcaagat cgatctcgtc gtcctgcgca atgggtcgcg acggcttttt tgcgccgccg
     4801 gcggcgcctg agggctcacc gggagcgtcc tcatcgggga tgtcgaattc gcccagatcg
     4861 agctcgtcat cgacggcctt ccgtttgggg gccgcgtccc gcccgtccgt cgcgtcctcg
     4921 tcgaatgagg cgtccaggag atcgtccccg gggtcgtgcg acccggtctt cgcgtcccgc
     4981 gcgggggcct catcgccaaa ccggtccgcg tggcgggcgc cagccgccgc cggggtcttc
     5041 tcggccatgc ggttgtcgat gatattgctg gtcgcgcact tggggcaggt aaaggcgaac
     5101 ttgcggtttt ccacctttga atcgtccacc gcgaacgcgg aattgcaatg agtgcagcga
     5161 ataatcatat caatctcctg ccggccttac cgccccggga aaaagcgctc gttgatatta
     5221 tgctggatga ccaagggaga aataacaagg aaaaaaacca tataccagcc gaacccgcgc
     5281 ccgttgagct cctcggcctt ttgcatgtat ttcaggttga tgtacaggct ccacaccccg
     5341 aacgtgagca ccacgaggag gacgttcgaa acgggattgt cgttaaacat ccggctggtc
     5401 cgcgcaagcc accaaaaata atacaggccc gccgtgaaaa ccaggagcag aaatagaata
     5461 accagattgt tctcgggcgc gtactcgttc ttcatagcgt tccccaccat cggctcacat
     5521 agaaaatagg cattccgacg aatcccagca aataaaccat gcgcaaaaaa cggaattacc
     5581 tcgatatttt attaacggaa tttctcattg aataatatac aactttttcg ccccagccgg
     5641 ggagcaccgt tcgcatgtcg cccgaaaaaa tgtattgtaa ttttcaatgc gtcatattcc
     5701 agtacgtatg cgcgccactc gcgcacccgg acgcgacacg aacaggcggt ctcccatggc
     5761 atctgcgaca tatttccatt acaccgatac gggcatgcgg cggcgcctcg ccgtcgcgat
     5821 cgcggcgatc atggtcgtgc tcgggttttt ctgcgcggac atctgcgcct cgctgcacgc
     5881 ctactacccc tatcatcggg cgctcacgct gggcctcctg ggcgcactcg gctgcctgct
     5941 ggcgggcctg gcgatcgggt cgcgattcta cgcgatcggg ggccgtcggg ggccgtttcg
     6001 catcctcgcg gtgctcatcg ccgccatcac cgcggccgga atcatcgcca ccgtcctgac
     6061 ggccccggag cgcgacctgc gcgccgagct cctgctcata tcgtcatttt ttcttgtccc
     6121 gctggtgggc ctgctgccct tcctcgccgg catgtatatc gcctatccgc tcaagctcgc
     6181 ttgcggcgac ttcatcgacg ggcagagcgc cctggcgccg ttcgtcctgg cgcttttcgc
     6241 cgggctcgcc gccggcgtcg ccatgttcgt cgcgtcctgc tatgtcgatg cgctgcgcgt
     6301 cctgccgctc gtcgcgattg cggcctccgc gccgttcgtg ttcgccatca atctcgactt
     6361 cagcccgagc gccgtcacca cggacgagtc cgcgccgccg ccgcagtcct cccatttccc
     6421 gggccccgat gcgctcgaac gcgccatcac cctgacgcgc tacgccgttc cgcttgcgct
     6481 ggcggtcctg ggatacgtgt ccctgcaacg gttcttcggc gacacctttc acatctcggc
     6541 ggccatcatc ggcttcgccc tcgcggccgt gcccgccggc ttcgccgctg cgcacctgct
     6601 gcgccgccta ccctggcgaa acttcgtcga gccgctcgcc ccgatcgcgt ttctcctgcc
     6661 cctctatgcc caggcgctct acgcgcacat atccttcacc gcggccacgc tcctgtatgc
     6721 gccggtgatg atcatattcg ggttctccct ctacagcgcc ctggagtcgt tacgggcgca
     6781 gtacgcccac ggccggcggt tcggcgtggt gatcgcctat gcggccgtcg ttccgatcgc
     6841 gctgttcgcc ctggccgcct ttgcgccgct ggtcaatgcc gcctatgtgg ccatcgtcgc
     6901 gtgcgcatcg ctcatgctcg tggcggcggc aaccgccgcc ggcttatcac gggcaaccac
     6961 cgtccaccag cgcatcgccc acgcggcgct cgccccggcg
//
LOCUS       DAORHF010000197         6999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10575_length_6999_cov_4.047811, whole genome shotgun sequence.
ACCESSION   DAORHF010000197 DAORHF010000000
VERSION     DAORHF010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10575_length_6999_cov_4.047811"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PKJ16_14110"
     CDS             complement(<1..532)
                     /locus_tag="PKJ16_14110"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV48175.1"
                     /translation="MFYRFGEITDLTSIAPLIGKKAESLVRMAAWGLPVPDGGCVTTR
                     GFDEFIDANNMRGIVDDIRSLMTARDEANALERAIEAQSLIIQGDIQPELMRHLAVLQ
                     EQNPASHFAVRSSGTKEDLVDASFAGQYTSILNVKSAEHLALAVKQCWASLFNDRVLR
                     YCLNKGIDFSSMGLAVV"
     gene            complement(724..3633)
                     /locus_tag="PKJ16_14115"
     CDS             complement(724..3633)
                     /locus_tag="PKJ16_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV48176.1"
                     /translation="MSTADLPRTYETYCKPKVAELLRAVRLDKVYHRAEGDYAYYTDE
                     AGAERKVIDFLGGYGSVLFGHNNPELVAVAKDSFDKKLPFSSQASCRGTAALLCEKLS
                     MMMHRRTRRNFVTTLASTGAEAVEAAIKHAEFVHQRKIEEIHSHFTRERLILRRKYRE
                     GELSIAPGMLQMIQERFGVPPHASFSRHMLAVRNHNYRIFRKPPTFLALNKSFHGKTS
                     GAVQLTHYTDYRTPFERIGIDVIFLEPGDKQALNRAVADSLIPYFWPCINDKGQITLE
                     EKLHVNISAFFIEPIQGEGGIRIIPKEYLEYYRAICTEHRIPLVFDEIQCGMGRTGTF
                     LFSEQQGVVADYYLLSKSLGGGLAKISAMMVHDEQYEREFGFIHSSTFAEDDHSAAIS
                     LAALNMLDRDETIFTACQERGEMLIKGLREIQTRFPGVIEEVRGCGLMIGLQFANLDR
                     APSSAFKMLSVQKLLGYVIAGYLLHEHGIRVAPTLSSSATIRLEPSAFISEADCALLL
                     TAIRRLCEVIYKRNIYELTRFIVNAESPGSTEEVGDYRTASTPVELPRTMRKVAFIGH
                     FIEARHMPLFDKSYERFTAEECERFLEKVYELVDPQIYEEIPVRSITGEMVSLNFIGF
                     IVDSKIISRYLLSGDVGPLHDKIETAVNIAVENGCQVMGFGGFTSIITRNCTSIINDS
                     IGLTSGNSFTVAIGLEALRKAAVQAGIDPTNGCLAALGATGNICSIYSEIMAEEVPRI
                     ILIGRPGSGARLRMVAADIYLSAFNQILAYESDVTAGTGDAKKKRALTGIAREIYDTK
                     SVRHLVGNYLGVERIGEWLLDHLSVEMMDALPVVCTEDMSMVKHANMIVCASNTPAPV
                     IYPNMLGEGPIVISDIAVPNDVDDSVLTERSDVMVIQGGVVRLPYNPDFMVGGIPLDP
                     GTSFACMAETILLGLTGIREHYSYGRITKVQVKKIQEIAKIHGFSLGRFKTERSF"
     gene            complement(4043..4441)
                     /locus_tag="PKJ16_14120"
     CDS             complement(4043..4441)
                     /locus_tag="PKJ16_14120"
                     /inference="COORDINATES: protein motif:HMM:NF021438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HNV48177.1"
                     /translation="MKRTMFTFSIIVVFAMVAIPLMSADGIVGKWKRNDGASVVEFKC
                     DGDNCRMIVVEANKANANVCNTGKNIIGYQTGSVKKTGDNVWQGTLFNPEDCKTYSAR
                     ATVSGNTLTLKGGYKIFGAYIGKTMTFKRQ"
     gene            complement(4692..5057)
                     /locus_tag="PKJ16_14125"
     CDS             complement(4692..5057)
                     /locus_tag="PKJ16_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48178.1"
                     /translation="MSTNLFKYEYLADSNVLVGVILSDNIEKDDSKAILDYAIEELKN
                     QPDSPNYIMDVRRVTKVSSYAIGVLMKALGVMKKTKGYMILVMTEALLQEIMLQHPEM
                     FDYYAVFHTIEDAVAFIRK"
     gene            5288..6439
                     /locus_tag="PKJ16_14130"
     CDS             5288..6439
                     /locus_tag="PKJ16_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV48179.1"
                     /translation="MDFKLTEEQSMVKDMCRKFADNELKPKAAHYDKTHEFPWEHVKK
                     LGEMGMFGVVYSEEYNGAGMDYPSYAIAVEELSRGCASTGVIISAHNSLCLSPINYFA
                     SPEIKKKVLPKLCTGEWLGCFGITEPNAGSDAAGTTTTAELKNGKWVLNGTKNFITNG
                     GVANVAVVTAVTDKGQGAKGLSFFIIEKGTPGFSVGRTEEKLGICASSTTELVFENCE
                     IPEENLLGKRGDGFKIAMHTLDGGRVGIAAQALGIAQAAFEESVKYANERQQFKQKLS
                     NFQAIQWMIADMATEIEAARLLVYQAATLIGTGERQRYSKYAAMAKLFASEVSHRVTH
                     KAIQIFGGYGYTREYPVERFYRDARITEIYEGTSEIQRLVIASNVIREF"
     gene            complement(6527..6871)
                     /locus_tag="PKJ16_14135"
     CDS             complement(6527..6871)
                     /locus_tag="PKJ16_14135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48180.1"
                     /translation="MIEVRTEEHGDVMIMHIKGEFYIENIQTVENTWNELVARKPKII
                     AINCHDLEFIDSSAIGTLVKFLNNAMNKKIKLIFYDLSKSIQQIFETARLNNFFTVTT
                     KSKFETQFLNDI"
BASE COUNT         1507 a         1959 c         1927 g         1606 t
ORIGIN      
        1 cgaccaccgc gagacccatg gaagagaagt cgataccctt attgaggcag tagcggagca
       61 cgcgatcgtt aaagagcgac gcccagcact gcttgaccgc aagcgcgagg tgctccgcgg
      121 atttcacatt gaggatgctg gtgtattgcc cggcgaacga ggcatcgaca aggtcttcct
      181 tcgttccgct gcttcggacg gcgaagtggc tggcggggtt ttgctcttga agcacggcga
      241 ggtgccgcat cagctccggt tggatgtcgc cctgtatgat gaggctctgg gcctcgatcg
      301 cccgctcgag tgcgtttgcc tcgtcgcgcg cggtcatgag cgaacggata tcgtccacga
      361 tgccgcgcat gttgttcgca tcgatgaatt cgtcgaagcc gcgcgtggtc acgcacccgc
      421 cgtcgggaac cggaaggccc cacgcggcca tgcgcaccag cgattccgcc tttttcccga
      481 tgaggggcgc aattgatgtg aggtcggtta tctcgccaaa gcggtaaaac atcgcacaac
      541 accacccgaa aacgaatgca ccggccgcga acgatgtgat cgcggcgcga ccataaccaa
      601 ttcactccat gagttctctt ggaacgcgtc cagcatgtat cgtgtttcca tcgggataaa
      661 actgaccgac gggctagact ttttggccat aacagaagta tggaccaaaa acacaagcac
      721 aaattaaaag ctgcgctccg ttttgaaacg cccgagcgag aagccgtgaa ttttcgcgat
      781 ctcctggatt ttcttcacct gcaccttggt gatacgtccg tagctgtaat gctcgcgtat
      841 gccggtaagc cccagcagga tggtctcggc catgcacgcg aacgaggtgc ccgggtcaag
      901 cggaatgccg cccaccatga agtccggatt gtacgggagc cgcaccacgc cgccctgtat
      961 caccatgaca tcgctgcgtt ccgtgagaac ggagtcgtcc acatcgttgg ggaccgcgat
     1021 gtcgctgatc acgatgggcc cctcccccag catgttcggg tagatcacgg gggcgggcgt
     1081 gttgctcgcg cacacgatca tgttggcatg cttcaccatc gacatgtcct cggtgcaaac
     1141 gaccggcagc gcgtccatca tctccaccga gagatggtcg agcagccatt cgccgatccg
     1201 ctccacgccg aggtagtttc cgacgaggtg gcgcaccgac ttggtgtcgt aaatctcccg
     1261 cgcgatcccc gtgagcgcgc gtttcttctt cgcgtcgccc gtcccggcgg tgacgtccga
     1321 ttcgtatgcg agaatctggt tgaaggccga caggtagatg tcggcggcga ccattctgag
     1381 acgcgcgccg ctgccgggac gcccgatgag gatgatgcgc gggacctcct cggccatgat
     1441 ctcgctgtag atgctgcaga tgttcccggt ggctcccagc gcggcgaggc atccgttcgt
     1501 tgggtcgatc ccggcctgca cggccgcctt ccggagcgcc tcgagtccaa tcgccaccgt
     1561 gaacgagttc ccgctcgtga gtccgatgga atcgttgatg atgctggtgc aattccgcgt
     1621 gatgatggac gtgaagcctc cgaagcccat cacctggcag ccgttctcaa cggcgatatt
     1681 cacggcggtc tcgatcttgt cgtgcaacgg acccacgtcg ccgctgagca gatagcggct
     1741 gatgatcttc gagtccacga tgaacccgat gaagttgagg ctcaccatct ccccggtgat
     1801 tgaacgcacc gggatctcct cgtatatttg cggatcgacg agctcgtaga ccttctcgag
     1861 aaagcgctcg cattcctccg cggtgaagcg ttcgtagctc ttgtcgaaca gcggcatgtg
     1921 ccgggcctcg atgaagtggc cgatgaacgc gaccttgcgc atcgtgcgcg gaagctccac
     1981 gggcgtcgac gccgtgcggt aatcgccgac ctcctcggtg gaaccgggac tctcggcgtt
     2041 cacgatgaac cgcgtgagct cgtagatgtt gcgcttgtag atcacctcgc agagccgacg
     2101 gatcgcggtc aggagcagcg cgcaatccgc ctcgctgatg aaggcggacg gctcgagccg
     2161 gatggtcgcg ctgcttgaga gcgtgggcgc cacgcggatc ccgtgctcat gtaaaaggta
     2221 acccgcgatc acgtacccca ggagcttctg caccgagagc atcttgaagg ccgacgacgg
     2281 cgcgcggtcg aggttcgcga actgaagtcc gatcatcagg ccgcagccgc gcacctcctc
     2341 gatcacgccg gggaatcgcg tctggatctc ccggaggccc ttgatgagca tctcgccgcg
     2401 ctcctggcag gcggtgaaaa tggtttcgtc ccggtcgagc atgttcagcg cagccagcga
     2461 aatggccgcg ctgtgatcgt cctcggcgaa ggtcgagctg tgaatgaagc cgaactcgcg
     2521 ctcgtattgc tcgtcgtgca ccatcatggc cgatatcttc gcaaggccgc cgcccaggct
     2581 cttggagaga aggtaatagt ccgccacgac gccctgctgc tccgagaaca ggaaggtgcc
     2641 cgtccgcccc atgccgcact ggatttcgtc gaagacgagc ggtatccggt gctcggtgca
     2701 tatcgcccgg tagtattcga gatactcttt ggggatgatc cggataccgc cctccccctg
     2761 gatcggctcg atgaaaaagg ccgagatgtt cacatgcagc ttttcctcga gggtgatctg
     2821 gcccttgtcg ttgatgcagg gccagaagta cggtatcaga gaatcggcga cggcgcgatt
     2881 gagcgcctgc ttgtcgccgg gctccaaaaa tatcacgtca atgccgatcc gctcgaaggg
     2941 cgtgcggtag tcggtgtagt gggttagctg cacggcgcct gacgtcttcc cgtgaaacga
     3001 cttgttgagc gccaggaagg tcggcggctt gcggaagatg cggtaattgt gattgcgcac
     3061 ggcgagcatg tggcggctga aactcgcatg cggcggcacg ccgaatcgct cctggatcat
     3121 ctgcagcatg cccggggcga tggacagctc cccctcgcgg tacttgcgcc ggaggatgag
     3181 ccgttcgcgg gtgaagtgcg agtgtatctc ctcgatcttc cgctggtgca cgaactccgc
     3241 gtgcttgatg gccgcctcga ccgcctcggc gccggtgctc gcgagcgtgg tcacgaaatt
     3301 gcgccgggtg cgccggtgca tcatcatgct cagcttttcg cacagaagcg cggccgtacc
     3361 gcggcaactc gcctggctgc tgaacggcag ctttttatcg aagctgtcct tcgcgaccgc
     3421 gaccagctcg ggattgttgt gcccgaagag caccgagccg tagccgccga ggaagtcgat
     3481 aaccttccgc tccgcaccgg cttcgtcggt ataataggca taatccccct cggcgcgatg
     3541 atagaccttg tccaaccgaa ccgcccgcag gagctcggcc accttgggct tgcaatacgt
     3601 ttcgtaggtg cggggcagat cggctgtact cattctgact cctcaacggt gatgaacatg
     3661 atacgaatta ctctattcaa tctatgccta ttgcgaaaaa tcaactcatt ttgtcgaatg
     3721 aaaaatgatt atataaattg gaccataatt atcaaattgt tcgttttgta ttgagatact
     3781 tgaaatggca actcgttatg cacagccgtt ctgcatcaga ccggcatgag ttgctgctcc
     3841 tattcaacat ccgacgcccc cacccttgtc cttcgagaga cgcattctcg tattattaac
     3901 gttgatgcta ccgtggggag tgattgagag agttttgaaa tcggcgcagg attaagcttc
     3961 ttattgccta ttaaatacaa acccctgcaa gatggctatc gagcaggggt ctttcaggca
     4021 actattattg gatctatgcc aattattgac gcttaaacgt catagttttc ccgatgtacg
     4081 ccccaaaaat tttatacccg cctttaagcg ttaaggtatt tccactgaca gttgctcttg
     4141 cagagtatgt cttacagtcc tcaggattaa acaaggtacc ttgccataca ttatctccgg
     4201 tttttttaac gcttccggtt tgataaccaa taatattttt accggtattg cacacattcg
     4261 catttgcttt gtttgcttct acaactatca ttctgcaatt atcgccatca catttgaact
     4321 caacaactga tgcgccatca ttacgcttcc atttgccgac aataccgtca gcactcatca
     4381 gcgggatagc gaccattgca aaaacaacga tgattgaaaa ggtaaacatg gtacgtttca
     4441 ttggttttct cctttagtaa aaatgttttc gtcgtgcgga tttttctaat gctaaatact
     4501 tgacgccatc tctttcccag tgtcaagaaa gtttggaaaa atagtgataa tcacaacaaa
     4561 gcgcaaatgc atatttccac tttgaaatat ccttgatttc gggcgattat aggcgtttaa
     4621 agacaaataa caagcttctt gtgcggcttc tacaggcact tgataaatac acttcactgg
     4681 gacggcgacg gctattttcg aataaacgcg accgcgtcct cgatcgtgtg gaacaccgca
     4741 tagtagtcga acatctccgg gtgctgcagc atgatctcct ggagcaacgc ctcggtcatc
     4801 accaggatca tgtagccctt ggtcttcttc atgaccccca gcgccttcat gagaacgccg
     4861 atggcgtatg aactgacctt cgtgaccctg cggacatcca tgatgtagtt cggcgaatcg
     4921 ggctggtttt tcagctcctc gatcgcgtaa tccaggatgg ccttggagtc gtctttttct
     4981 atgttgtcgc tgaggatgac gccaaccaga acattcgaat ccgcgaggta ttcgtattta
     5041 aaaagattgg tgctcattca cttcctccga ctgcttcctc ttgaacggtt tcactcatac
     5101 tggatatatc gtaccaatcg gtgcggctcc tgtcaataaa aagaagtttt tgctttttaa
     5161 ataccttgac aaacctgcat attattcgga gttatgtacg aaaattaaag tatggtctgt
     5221 tttatgattt cgcaaaacgc acatctccat tctcaatcaa ataccacaca tggcaaagga
     5281 gaccgcaatg gattttaaac tcaccgaaga gcagtcgatg gtaaaggaca tgtgccgcaa
     5341 gttcgcggac aacgagctca agccaaaggc ggcgcattac gataagacgc acgagttccc
     5401 ctgggagcac gtcaagaagc ttggcgagat gggcatgttc ggcgtcgtgt attccgaaga
     5461 atacaacgga gcgggcatgg actatccgag ctatgccatc gccgtcgagg agctctcgcg
     5521 tggatgcgcc tccaccggcg tcatcatttc cgcgcataat tcgctctgcc tgtcgccgat
     5581 aaattatttc gcgtcccccg agatcaaaaa aaaggtgctc ccgaagctct gcaccggcga
     5641 atggctcggc tgctttggca tcacagagcc caatgccggc tccgatgcgg cgggcactac
     5701 caccacggcc gagcttaaaa acgggaagtg ggtgctcaac ggcaccaaga actttataac
     5761 gaacgggggc gtggccaatg tcgccgtcgt gaccgcggtc accgacaagg gacagggcgc
     5821 caagggtttg agcttcttca tcatcgaaaa gggaacgccc ggattcagcg tgggccggac
     5881 cgaggagaaa ctcggcatct gcgcctcgtc gaccaccgag ctggtattcg agaattgcga
     5941 gattcccgag gaaaatttgc tcggaaagcg tggtgacggc ttcaagatcg ccatgcacac
     6001 cctcgacggc gggcgggtcg gcatcgccgc ccaggccctc gggatcgcac aggcggcctt
     6061 cgaggagtcc gtgaagtacg ccaatgagcg ccagcagttc aagcagaaac tttcgaactt
     6121 ccaggccatc caatggatga tcgccgacat ggccaccgag atcgaggcgg cgcggttgct
     6181 ggtctatcaa gcggcgacac tcatcggcac tggagagcgc cagcgctatt ccaaatacgc
     6241 cgcgatggcg aagctcttcg cctcggaggt atcgcaccgt gtaacgcaca aggccatcca
     6301 gattttcggc ggttacggct atacgcgcga gtacccggtg gagcgtttct atcgcgacgc
     6361 gcgcatcacc gaaatctacg agggcacctc ggagatccag cgtctggtca tcgcgagcaa
     6421 tgtgattcgc gagttctagc gccgagtacc atttctccag tatactgcaa aaacggcatg
     6481 gtgcaatcca ccatgccgtt tttttgatta gtactggtga gcgatgttag atatcgttga
     6541 gaaactgcgt ttcgaacttc gattttgtcg taaccgtgaa gaagttgttc aaccgtgccg
     6601 tttcgaaaat ctgctggatg gatttgctca gatcgtagaa gatcaacttg atctttttgt
     6661 tcatcgcgtt gttcaagaac ttcaccagcg tcccgatggc cgacgaatcg atgaactcca
     6721 gatcatgaca gttgatggcg atgattttgg gctttcgggc gacgagctcg ttccaggtgt
     6781 tttctaccgt ctggatgttc tcgatataga actcgccctt gatgtgcatg atcatcacat
     6841 cgccatgttc ctcggttctg acctctatca tggcaccttc ctgtttgtgt taatgtcccg
     6901 tcacgttcgg gaatcgcgcg cgatccggaa gttgcgcgat taggtgttga caattttcaa
     6961 tccgtcggta cagtatcaag agtatcgtgt ctagcgcac
//
LOCUS       DAORHF010000198         6938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10717_length_6938_cov_4.848613, whole genome shotgun sequence.
ACCESSION   DAORHF010000198 DAORHF010000000
VERSION     DAORHF010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10717_length_6938_cov_4.848613"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(201..1043)
                     /locus_tag="PKJ16_14140"
     CDS             complement(201..1043)
                     /locus_tag="PKJ16_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HNV48181.1"
                     /translation="MASRKQCFDEAVRAASEEFLTESFTRTYSSIFDAQDEMRTALKG
                     SGYAPTFSESGDPIYSLPDLRAHDALALCVIDSLKRIAEIIRAQHPGIDFTPVIESTA
                     FDREALSELVGSFASQSMNQIESLAARYRVGAEELVFLLTYAISPCYAFARQQYRGTI
                     ETQTLERYRCPFCGSLADMALIHQDAGTRYLHCSRCDLIWQVKRVACAVCGNENIDDL
                     GYFELDGRESDYRISYCDHCHGYIKTVLIAKFDDPLMRDVVIDNLMTANLDHAAIHRG
                     YSRP"
     gene            complement(1043..1258)
                     /locus_tag="PKJ16_14145"
     CDS             complement(1043..1258)
                     /locus_tag="PKJ16_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HNV48182.1"
                     /translation="MGMPGMWELVIIFLIVLLVFGAGKIPKLAKDIGSGIREFKKSVS
                     GEDDSRSQKNDTTASSNNNSNDNSAKS"
     gene            complement(1356..3086)
                     /locus_tag="PKJ16_14150"
     CDS             complement(1356..3086)
                     /locus_tag="PKJ16_14150"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HNV48183.1"
                     /translation="MKQTIFYFDSTARRLFVEKVDEQGEKHVSLSSPYNFVTDNEIVA
                     RVIEIESPEDIAKQIDKGQTYYTAENYTKFRAGDGIVFDETARAYRASTYGFAIFDRD
                     RSLVRLLSPLQISKDKVSAFYVIFPTKRGVLPTYKHIEDALFNAKIVAIVDKGEIEQQ
                     LAAIDPTKPAVHRVRVARGKSPINGYDEYFIPLIKLEKKAGKVLDDGRIDFREVESIQ
                     EIKKGQEILQRFPAVKAVDGYTIYGEKILAEIEKKTGYLKGENIVKAETEENVFVSSI
                     DGCLEVEGQLISVLQIARIKGNVDYDSGNIDFNGSVHIMGSVMPGFSVKAKGNVIIDK
                     NADDATIEAEGDVTVKLGITGKGQSRVIAGGKVRAGYVLNAHIEAVGEIFVDDSIINS
                     WVFSNDKITVTSKHGQIISGEVTARHDVIANVIGNTQENTTTVSVGKSLFIERELLDI
                     RADMEKARTSVNEIIIKIKTSFGEGLFENPKDFIAILPTVKKKQCLLLLKELSDSNKE
                     IKHLAELRAQAEEKLKLDREPSIVANNTIYPGAVLMVKKRKRVINEVMQNVKFFEDPA
                     EKVIRFTSAI"
     gene            complement(3153..4697)
                     /locus_tag="PKJ16_14155"
     CDS             complement(3153..4697)
                     /locus_tag="PKJ16_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HNV48184.1"
                     /translation="MKVVTARQMQSIDRATIDSMGIPGVVLMAQAGRAVADRVLSLCS
                     PCSSVAVICGTGNNGGDGFVVAYHLWNHGIQTTAYLLGESARLTPDARIFHDTCVRAE
                     ITVHVVSGEVHLADITLSEYDLIVDAMTGTGFSGPARGLLEAAIGAINECGVPVVSVD
                     LPSGLSSDGEAPSGPAIRAQHTVTIGLPKISTVTHPCAAYCGELTIADIGFPRALIDT
                     NDIRTELVDERFVRAHYRRPTDPDLHKGKAGSVLCIGGFDGMEGAIMMSIAAAFETGI
                     GLATLLTTRSARASIAGRIPELMTMALPDDDGTSAVRSLEILPTNRKYSSALIGPGMG
                     RDDYSARVFAAFMLQLPATSISSVVIDGDGLYHLADYLATHALPHGISFVVTPHVSEA
                     ARLAGSTVEAISSNRFETAMSLAAKTGAVAVLKGPSTISSDGHQALINTTGDYRLATA
                     GSGDVLAGILVALLNAIEPLVAAGIAAYVHGAAADRFVRETGALSIKATDVISRIRTI
                     LSGSAQ"
     gene            complement(4722..5801)
                     /locus_tag="PKJ16_14160"
     CDS             complement(4722..5801)
                     /locus_tag="PKJ16_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48185.1"
                     /translation="MMIRLLSLCLLIMSTILSLSCGKWKVDTHRSGKIADIPFGEAPG
                     SIAVIRNPSGFIDITFLVRLSRGKILAADNLLKRVQVLDRGGSPELVIGSQKSAGKPG
                     AGQRIAPFSFSIIGEMVLDSEGNLYVQNRFSSAQARRKSSIDDLNITPSYVLVFDRKG
                     ALKYTLGQTGASDIPFYYIESIDVDTKDRLLVVSRSLNTWSVSRYTGKRRDSYANLGK
                     LDFTEKEKEGTFTGKIELVRTMHNGEGAVISVAYYHQSRFKYRKIFTYAIDQEKIVRT
                     ALSTPDPKNELFSIIDSRYLYLWNVENKKIKFMVCNLDGGIVNNILLKMPDDFDSFGE
                     ILIDESGLFYSISARKDRITVIEWK"
     gene            6171..6380
                     /locus_tag="PKJ16_14165"
     CDS             6171..6380
                     /locus_tag="PKJ16_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV48186.1"
                     /translation="MPYIVIDSERCKGCYFCVKYCPRNVIRESTMHNKTGYFPAEFIH
                     EQEDRCTGCKTCAIMCPDTAIEVFK"
     gene            6380..>6938
                     /locus_tag="PKJ16_14170"
     CDS             6380..>6938
                     /locus_tag="PKJ16_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNV48187.1"
                     /translation="MDRRLTKGNIALAEGAIQAHCRYYFGYPITPQNDIPEYLSRNLP
                     KVGGVFIPAESEIASINMVMGAAASGKRAMTSSSGPGISLMQEALSYMAGSDIPCVVV
                     NIMRSGPGLGGIAPTQGDYSQAVHGGGHGDYHSIVLAPASVQEMFDLTIKAFELADTY
                     RNPAMIIADAMIGQFQEPCRLTPREP"
BASE COUNT         1508 a         1903 c         1954 g         1573 t
ORIGIN      
        1 cacgcaaccg gatcgaatga cggcgtgcaa tacacaaatg cgcgcggacg taccttgtcg
       61 acaagagagc ggacaaatga aggatcgctc accgagccgc gatcatcatc gaatgaataa
      121 acctggaaat tgtttctgag tatcggcgcg atcgatgcgc caagaagcgt cgagtcgcct
      181 gagaggatca ccatggtcgg tcaggggcgc gaatacccgc ggtggatggc cgcatggtcg
      241 agattggccg tcatgagatt gtcgatgacg acatcgcgca tgagcggatc gtcgaacttt
      301 gcgataagga ccgttttgat gtatccatgg cagtggtcgc agtacgagat gcgatagtcc
      361 gattcacgcc cgtccagctc gaaatagccg agatcgtcga tattctcatt gccgcatacg
      421 gcacatgcga cccgtttgac ctgccagatg agatcgcacc gtgagcagtg cagataccgg
      481 gtgccggcgt cttggtgtat aagcgccatg tcggcaagcg atccgcagaa ggggcaccga
      541 taccgctcca gggtctgtgt ctcgatagtt ccccggtatt gttgacgggc gaatgcgtaa
      601 cacggtgaaa tcgcatacgt caaaagaaac acgagctctt cggcgccaac gcgataccgg
      661 gctgccaggg attcaatctg gttcatcgat tgcgacgcga acgagccgac gagctcggaa
      721 agggcttcac gatcgaacgc ggtcgactcg atgacgggag tgaaatctat acccgggtgc
      781 tgcgcacgta ttatttcggc aatgcgtttg aggctgtcga tgacgcataa tgcaagtgcg
      841 tcatgtgcgc gaaggtccgg cagcgaataa atcggatcac ccgattcgga aaatgtcggg
      901 gcgtatccgg atcccttgag cgcggtgcgc atctcgtcct gcgcatcgaa aatcgatgaa
      961 tacgtgcgcg taaacgattc tgtcagaaat tcttccgacg cggcgcgtac ggcctcgtcg
     1021 aaacactgct ttcgagaggc cattaactct tggcgctatt gtcgttggag ttgttattcg
     1081 aggatgcggt ggtgtcgttt ttttgagaac gggaatcgtc ttcaccgctt accgatttct
     1141 tgaattcccg aatgccgctt ccgatgtctt tcgcgagctt tgggattttg cctgcgccga
     1201 aaacgagcag gacgataaga aaaatgataa cgagttccca cattcctggc atgcccatag
     1261 tgtgcctcct gaaatatctg tgctcagagt gggcatgcct cgatttttgt caaataaatt
     1321 cgatcaataa atggcgcggt gggcgaaaaa aggggctaaa tggccgatgt gaatcggatc
     1381 accttctcgg ccgggtcctc gaagaacttc acgttctgca tgacctcgtt gatgacccgc
     1441 tttcgtttct tgaccatcaa aacggctccg gggtagatcg tattgttcgc cacgatggac
     1501 ggttcccggt ccagcttgag tttttcctcg gcttgggcgc gtagctcggc aaggtgcttg
     1561 atttccttgt tcgaatccga aagctccttc aggagcagga ggcactgttt ctttttaacg
     1621 gttggcagga tggcgataaa atccttcgga ttctcgaaga gcccttcgcc gaaactcgtc
     1681 tttatcttga tgatgatctc gttcaccgat gtccgcgcct tctccatgtc ggcgcgaatg
     1741 tccagaagct ctctttcgat gaacaggctc ttgccgacgg acaccgtcgt cgtgttctcc
     1801 tgcgtattgc cgataacatt cgcaatcaca tcatgacgcg cggtcacctc gccgctgatg
     1861 atctgtccgt gcttcgaggt gacggtaatc ttgtcgttcg agaacaccca gctgttgatg
     1921 atggaatcgt ctacgaatat ctcgccgacg gcctcgatgt gcgcgttaag cacatacccg
     1981 gcacggacct tcccaccggc gataacgcga ctctggcctt tcccggtgat accgagcttg
     2041 acggtcacgt cgccctccgc ctcgatggtc gcgtcatccg cgtttttgtc aatgatcacg
     2101 tttcccttcg ccttgacgga aaacccgggc atgaccgatc ccataatgtg aacggaaccg
     2161 ttaaaatcga tattgccgga atcgtaatcg acattgccct tgatgcgcgc tatttgcagg
     2221 acggagatga gctgtccctc gacctcgagg cagccatcga tggacgacac gaacacgttt
     2281 tcctcggttt ccgctttgac gatgttctcg cccttgagat agcccgtttt cttttcgatc
     2341 tcggcgagga tcttctcccc gtagatggtg taaccgtcta cggccttgac cgctggaaat
     2401 ctctggagaa tctcctgccc cttcttaatc tcctgaatcg attcgacctc gcggaaatct
     2461 attcggccgt cgtcgaggac tttgcccgcc tttttctcga gtttaatgag cgggatgaag
     2521 tattcgtcat atccgtttat gggcgacttg ccgcgagcga cgcgaacgcg atgcactgcc
     2581 ggcttcgtcg gatcaatggc ggcaagctgt tgttcgatct ctcccttgtc cacaatggcg
     2641 acaattttcg cgttaaacag cgcatcctcg atgtgcttgt aggtcggcaa cacaccgcgt
     2701 ttagtgggga agatgacata aaacgcgctt accttgtcct tcgatatctg gagcggcgac
     2761 aggagccgta ccaacgatcg atcgcggtcg aagatcgcga acccataggt ggatgcacga
     2821 taggcgcgcg ccgtctcgtc gaacacaatg ccatcgccgg cgcgaaattt ggtataattc
     2881 tccgcggtat aataggtctg gcccttgtcg atctgtttcg cgatatcctc gggtgattcg
     2941 atctcgatca cgcgggcgac gatttcgttg tccgtgacga agttataggg cgacgacagg
     3001 gagacgtgct tctcaccttg ctcgtcgacc ttctcgacga agaggcgtcg ggccgtgctg
     3061 tcaaaataaa aaatcgtctg cttcatctcg gttttcggcc acaggtgccg cttatactat
     3121 tgtcgtaccg catctttcaa aaactaaacg cattattggg ctgatccgga caaaattgtc
     3181 cggattcggc taatgacatc ggtcgccttg attgagagcg ccccggtttc ccgcacgaat
     3241 cgatcggccg cggccccatg cacatacgcg gcgatcccgg ccgcaacgag cggctcgatg
     3301 gcattcaaca gcgcgacaag aatccccgca aggacgtcgc cggaacccgc cgttgcgagc
     3361 cggtaatcgc ccgttgtatt gatgagcgcc tgatgtccat cgcttgatat cgttgacgga
     3421 cccttgagca ctgccacagc gccggtttta gccgccagcg acattgcggt ttcgaagcgg
     3481 ttcgatgaga tcgcctccac tgtcgatccg gcaagccgcg ccgcctcgga aacatggggc
     3541 gtgacaacaa acgaaatacc gtgaggaagc gcgtgtgttg caagataatc cgcaagatgg
     3601 tagagcccat cgccgtcgat gacgacggag gatatcgagg tcgcgggaag ctgcagcatg
     3661 aacgcagcga acactcgtgc ggaataatca tcccgcccca tgccgggacc gatcagtgcg
     3721 gaggaatatt ttcgattcgt cggtagtatc tcgagcgatc tcacggcaga cgtaccatcg
     3781 tcatcgggca atgccatggt catgagctcc gggattcgtc cggcgattga tgcgcgggcg
     3841 gagcgggtcg tcaggagcgt tgccaaaccg atgcctgtct cgaaggcggc agcgatcgac
     3901 atcatgatgg cgccctccat gccgtcgaat ccgccgatgc agagaacgct gcccgctttc
     3961 cccttatgga gatcgggatc ggttgggcga cggtagtggg cccggacaaa gcgctcgtcg
     4021 acgagctcgg tgcggatatc gttcgtgtcg ataagcgccc tcggaaagcc gatgtcggcg
     4081 atcgtaagct caccgcaata tgccgcgcat ggatgtgtca cagtcgatat cttcggcagg
     4141 ccgatcgtta cggtgtgctg cgcccggatc gccggtccgc tcggcgcctc gccatcggac
     4201 gatagcccgc tgggaagatc cacggaaacg acgggaacgc cgcattcatt gatcgcgccg
     4261 atcgccgctt caagcaggcc gcgggcggga ccggaaaatc cggtgccggt catcgcatca
     4321 acgattagat cgtattcgga taaggtgatg tcggcgagat gcacctcgcc tgaaacgaca
     4381 tgaaccgtga tttccgcacg aacgcaggta tcgtggaata tgcgggcatc gggagtcagc
     4441 cgtgcgctct cgcctaacag atacgccgtg gtttggatgc cgtggttcca gagatggtac
     4501 gcgaccacga acccatcgcc cccattgttt cccgttccgc agatgacggc gacggacgaa
     4561 catggcgaac acagggatag gacccgatcc gcgaccgccc tgcccgcctg cgccatgagc
     4621 acgacacccg ggatgcccat cgagtcgatg gttgcgcggt cgatcgattg catctggcgc
     4681 gcggttacca ctttcatgac gtcaccgatt ccgcatgagc gctacttcca ttcgatgacc
     4741 gttatccggt cctttcgggc gctaatggag taaaacagcc ccgattcgtc gatgaggatt
     4801 tctccgaagg aatcgaaatc gtccggcatt ttgagcaaga tattgttcac gatccccccg
     4861 tcgagattgc agaccatgaa tttgatcttt ttattctcga cgttccacag gtacagatag
     4921 cggctgtcga tgatcgagaa gagttcgttt ttcggatcgg gcgtcgatag cgccgtccgc
     4981 acgatctttt cctgatcgat ggcgtatgta aaaatctttc ggtacttgaa ccgtgattga
     5041 tgatagtacg caacggagat caccgcccct tcgccgttgt gcatggtgcg caccaactcg
     5101 attttcccgg taaaggttcc ctccttctcc ttctccgtga aatcgagctt gccgagattc
     5161 gcgtaactgt cgcggcgctt tccggtgtaa cgcgaaacac tccaggtatt cagcgagcgc
     5221 gagacgacaa gcaggcggtc cttggtgtcg acatcgatgc tctcgatgta gtaaaatggg
     5281 atatcggatg ccccggtttg gccgagcgtg tatttcagcg ctcccttgcg atcgaacacc
     5341 agaacatagg atggggtgat gttcagatcg tcgatgctgc tcttccgccg cgcctgcgcg
     5401 gaggagaagc ggttctggac atagaggttg ccctcggaat ccagcaccat ctcgccgatg
     5461 atactaaagg aaaacggagc gatgcgctgg ccggcgccgg gctttccggc gcttttctgg
     5521 gagccgatga cgagctcggg cgaaccgccg cgatcaagca cctggacccg tttcagcagg
     5581 ttgtcggccg caaggatctt tccgcgtgaa agccgaacca aaaatgtgat gtcgatgaaa
     5641 ccgctcggat tgcggatcac cgcgatgctg ccgggcgcct cgccaaaggg tatatcggcg
     5701 atcttcccgg atcgatgagt gtccaccttc cacttgccgc acgaaagcga aaggatggtg
     5761 ctcatgatca gaaggcatag cgagagaagt cgaatcatca tggcgcccat tgcaattatt
     5821 gaagttaacg atcgtgttcg cacgtatatg aataaatggt attttgatca actattttaa
     5881 aatacataac agagaagtat ttgtcaaata aaaaagccca tagggattaa ttcgttttgg
     5941 aatgttgatt ccgtgaccgt tgagatgcaa cacgagaatc gttttgatga acacacggat
     6001 gcgatgaatc atcgatgttt gcgatatgcg atgagtgtca ataatcatca aataatcggg
     6061 atgttcatat taatttcaca agaaagtatt tacagaatcg ctgcatcaca tttcattgac
     6121 gcagttgtac cccaagcggg catgtatcag aacgacagcg taatgggact atgccgtata
     6181 tcgtaatcga cagcgaacgg tgcaagggat gttatttctg cgttaagtac tgtccgcgca
     6241 atgtcattcg cgagtccacc atgcacaaca agaccgggta tttccccgcc gagttcatcc
     6301 acgagcaaga agaccgctgc accgggtgca aaacctgcgc gatcatgtgt ccagacaccg
     6361 ccatcgaggt gttcaagtga tggaccgacg cctcaccaag ggaaatattg cgcttgccga
     6421 gggcgccata caggcgcact gtcgatatta tttcggctat cccatcaccc cacagaacga
     6481 tattcccgag tacctgtcgc gcaacctccc gaaggtcggc ggcgtgttca ttccagcgga
     6541 gagcgagatc gcctcgatca atatggtgat gggtgcggcg gcctccggga aacgcgccat
     6601 gacctcgtcg tcggggccgg ggatatccct catgcaggag gccctgtcgt acatggcggg
     6661 ttccgacatc ccctgcgttg tggtgaacat catgcgcagc ggtccgggtc tgggtgggat
     6721 cgcgccgacc cagggcgatt acagccaggc ggtgcacggc ggcggacacg gtgattatca
     6781 cagcatcgtg ctggcgcccg cgtccgttca ggagatgttc gacctcacga tcaaggcgtt
     6841 tgagctcgcc gacacctatc gcaatccggc catgatcatc gcggacgcca tgatcggaca
     6901 gttccaggag ccctgccggc tcacgccgcg ggagccgg
//
LOCUS       DAORHF010000199         6892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10826_length_6892_cov_4.872166, whole genome shotgun sequence.
ACCESSION   DAORHF010000199 DAORHF010000000
VERSION     DAORHF010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10826_length_6892_cov_4.872166"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..3049
                     /locus_tag="PKJ16_14175"
     CDS             128..3049
                     /locus_tag="PKJ16_14175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HNV48188.1"
                     /translation="MMAQRETHTACTGIEIGAVSVKWVLDGADGTTAVTHRHEGDPRT
                     KLRELFADESPGGNQRYVVTGQTARQLLDLPYRPETECLERALAHHGILPDILLSLGG
                     ETFTVYTLRSGVVKNVLSTTKCAAGTGEFIVQQLQRMDYSLEEGIAASASGTRVDLAT
                     RCSVHCKSDSTHKLNKGECTREDIVRSLIEDLARKVCRMIDQAQWPAGTIVISGGVSL
                     NEPFVSALKEQLPDSEIRILPESPYLEAFGAYLLCRDHDGVDAVVPPDRRIRETHITY
                     TQLRPLRESEGLLDYRVVSAEDRRIIDGDPYVLAVDAGSTTTKAVLYNSADGSIGAAC
                     YLRTHGNPVDAARRCLEELTRLTEGRSVRIIQAATTGSGREMVSVYLNNCLAFNEILA
                     HARAASAEVGGVDTVFEIGGQDAKYISFIDGVPVDYGMNEGCSAGTGSFLEESISVDM
                     NIPVHEISGRAIASDRPISFGERCAAFINTDLRNALQQGAARDDVIAGLVYSIVDNYI
                     SRIVGIRPIGETVLFQGGVALNRAVALAMAARSGRRIIVPRHPELMGCVGAALMASER
                     VSRGEIPETNLSLSEMAQTSITTGAPFTCAACENRCEIKRIIVRDTTYPFGGLCSLYE
                     NKRHASTARDTAKDFVSLRNRLMFEEFAPPPLERPRGTIGIPLALSTYEYFPYYAALA
                     HELGFNITLSSENRAGNQKTMGDICYPCEIVHGAVDDLLRAGVDYILLPHVIEAAIPE
                     GYLHSYLCINTGTIANIVMAAFEGLDDTLLRPHIAFSDHLTATTEQEIVRMGARLGVS
                     KKTAIHAARVARDRQREYCGRYAAMGRSVLEEIGDEPAIIVAGRPYVYGSNEANLALP
                     RKLISRGYHAIPMDLLPPIGDSVYERNVWHYGQQILNAIEHVKRKPSLHICLVSCFSC
                     FPDASIYHLVRTELSGHTFCYLEVDSHTAHAGFETRVGAFLDIIEERERAMRHRAGRT
                     A"
     gene            3046..4455
                     /locus_tag="PKJ16_14180"
     CDS             3046..4455
                     /locus_tag="PKJ16_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48189.1"
                     /translation="MSMMMEGPTAPPVLQARFSDDSTHIIDSDGNRVPLTDPRVVLVW
                     TIDCSPYVQRLIVSFMRHHGIRCRVSADTGKATLEYAKRISSGRECIAFRSIAGSIIR
                     DLAESRNPDEITIYYNHDQHGPCQNGAWPEIWRMYQHAINLRNVVLFSNFNFRNNYWG
                     LGDELGNELMTAIVFGDCFDEAEGVLRCIAKDAGAALDEFNRAIDTVVDAAAGGIAPA
                     FKAFKEWSRRMAAIPLSAGLDETPRVLIFGGLNTRLVHHPITTYFIEIGVIPKIVDFG
                     EITIANESEPALRYNYMRGRVRARDYMRFGPVLLDFFRPSSDRRAVWEALRARLAVTL
                     IEKMVRRYRRVAHTSGLIHDPHIPHEEVVYAGDRLVTSISYAETVLIAGRYVKTIESG
                     AYDGLVQVGVFGCPPTINAHAVLRPLANKSEVPYVALDVEGPWISANQTVLLESLAVQ
                     ARRERARKIAARTTRPSGR"
     gene            complement(4433..5683)
                     /locus_tag="PKJ16_14185"
     CDS             complement(4433..5683)
                     /locus_tag="PKJ16_14185"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV48190.1"
                     /translation="MSKSIVGHFKGKDTPHILIEVNQKCNLSCKACYKNRGTYTKPLA
                     LIKEEVDFAFTQRNLEAVTLAGGEPTLHPELHEVIRYIKAKGVKVNVLSNGLTLTDEL
                     LRQYNNAGLDFIHVHVDSHQKRPDAVHARTEKELDPIRDEILKRIKRNGIMPALSLTL
                     YKSTLSQLRDVVDYVLATEYAPCVLVTLYSDTRGLSEKFSKRVKSSKNIIKTSKSHGE
                     EEVLGADIEAYMKQHYGLDCLLHYSSNKDTNAKRWLFYIVLSIIRPDKTFEYISASYN
                     YLKFQMALDKLHNAIKKRYNFAIFPESKALLVFAMVLSALMSLQPRTIATTARLLWML
                     MKPGNTISFNQFYFQQPAGLNENGELEFCAACPDATIRNGLVVPVCVADVLSPVIEKG
                     PNDEKRNRMLLDLGLDSINVPKAG"
     gene            5982..>6892
                     /locus_tag="PKJ16_14190"
     CDS             5982..>6892
                     /locus_tag="PKJ16_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV48191.1"
                     /translation="MLKPADIMDNERYVDIKEENLENFSPKDFFKFADDPIDKKTAQF
                     SKYFNHMLTEGFIMREVRSPASKKVRIYERSSRKLIDVLMLGSNNYLDFANEPYIINK
                     TIQAICEYGIGCGGPPLLNGTTSLHRRLEGRLAAMKGTEDAMVYSSGYAANVGWATGL
                     LNEGDFLVYDWQSHASLHDSVKMGKFTGVRFLHNDMEDLDLQLTTVRRNSPKGNVVVC
                     VEGVYSMDGDIAPLPEIKALCKKHRALLCIDDAHGTGVLGKNGHGTQEHYDMMGEVDI
                     IMGTFSKSFGVTGGFIASSKKIIDYLR"
BASE COUNT         1373 a         2025 c         2088 g         1406 t
ORIGIN      
        1 caacggcgag gctgagttcg gcgcgcgggt cttcatcaat tggggcggca gtacatcgga
       61 gtttgggacg ctcatgcagc ggatcatggg acttgcgtat atccaaaagt ctttctaggc
      121 cggggtgatg atggcgcaac gcgaaacgca cactgcgtgc acgggcatcg aaatcggggc
      181 ggtatcggtc aagtgggtgc tcgacggcgc cgacggaacg accgccgtga cgcaccgaca
      241 cgaaggcgac ccgcgcacga aactcaggga gcttttcgcc gatgaatcgc ccggcgggaa
      301 tcaacggtat gtcgtgaccg gacagaccgc tcgacaactg ctcgacctcc cgtaccgccc
      361 ggagaccgaa tgtcttgagc gcgccctcgc gcatcacggg atattgcccg acattcttct
      421 gtcgctcggc ggcgaaacat tcacggtgta cacgcttcga agcggcgtcg tgaagaacgt
      481 cctctcgaca acaaaatgcg ccgccgggac gggcgagttc atcgtccagc agctccagcg
      541 gatggattac agcctggagg aggggatcgc ggcctcggcg tcgggcaccc gcgtcgatct
      601 cgcgacacgc tgctccgttc attgcaaatc ggattccacc cacaagctca acaagggcga
      661 gtgcacgcgc gaggacatcg tccgctcgct catcgaggac ctcgcgcgca aggtgtgccg
      721 catgatcgac caggcgcagt ggcccgcagg aaccattgtg atttccggcg gcgtctcgct
      781 gaacgagccc ttcgtctccg cgctcaagga acagctcccc gactcggaga tccgcatcct
      841 gccggagagc ccgtacctcg aggcgttcgg cgcgtatctg ctgtgccgtg accatgacgg
      901 cgtcgacgcc gtcgtccctc ccgaccggcg catccgcgaa acgcacatca cctataccca
      961 gcttcgtccg cttcgcgagt cggagggcct gctcgactac cgcgtcgttt ccgccgagga
     1021 ccggcgcatc atcgatggcg acccctatgt tctcgccgtc gacgccggat caacaaccac
     1081 caaggcggtc ctgtacaatt ccgccgacgg ctcgattggc gcggcatgct atctacggac
     1141 gcacgggaac ccggttgacg cagcccggcg atgcctcgag gaattgacgc gtctcacgga
     1201 ggggcgctcg gtgcggatca tccaggcggc gacgaccggc tccggacgcg agatggtgtc
     1261 ggtgtatctg aacaactgcc tcgcgtttaa cgagattctc gcacatgcgc gcgccgcgtc
     1321 ggcggaggtc ggcggcgttg atacggtctt cgagatcggc ggtcaggacg cgaaatatat
     1381 ctccttcatc gacggcgtcc ccgtcgatta cgggatgaac gagggatgct ccgcaggcac
     1441 cgggagcttt ctcgaggaat cgatttcggt cgacatgaac attcccgtgc acgagatcag
     1501 cgggcgcgcc atcgcgagcg accggccgat ctccttcggc gaacgctgcg ccgccttcat
     1561 caacaccgac cttcgcaacg ccctccagca gggtgccgcg cgggacgatg tgatcgccgg
     1621 gctcgtctac tccatcgtgg ataactacat atcgcggatc gtgggcatcc ggcccatcgg
     1681 cgagacggtg ctctttcagg gcggcgtggc gctcaatcgc gcggtggcgc tcgcgatggc
     1741 ggcgcgctcg ggcaggcgca tcatcgtccc ccgacacccg gagctgatgg gctgtgtcgg
     1801 cgccgcgctc atggcgagcg agcgcgtatc gcgcggagag attcccgaga cgaatctgtc
     1861 gctctccgag atggcgcaga cctccatcac gacgggcgcg ccgttcacct gcgccgcctg
     1921 cgaaaatcgc tgcgagatca agcggatcat cgttcgcgat acaacctatc ccttcggcgg
     1981 tttgtgctcg ctgtacgaaa acaagcgcca tgcaagcacg gcgcgggata ccgcaaaaga
     2041 ttttgtgtct ctccgcaacc gcctcatgtt cgaagagttc gcgccgcctc cgctcgaacg
     2101 gccgcgcggg accatcggca tccctctcgc gttgagcaca tacgagtatt ttccctacta
     2161 cgcggcgttg gcgcacgagc ttggcttcaa tatcacgctc tcgagcgaaa accgcgcagg
     2221 aaaccaaaag accatgggcg atatctgcta tccctgcgag atcgtccacg gcgcggtgga
     2281 tgatctgctt cgcgcggggg tcgattacat tctcctgccg catgtgatcg aggccgcgat
     2341 cccggagggc tatctgcatt cgtatctgtg catcaatacg gggaccatcg ccaatattgt
     2401 gatggcggcc ttcgaggggc tcgatgatac gctgcttcgg ccgcatatcg cgttttccga
     2461 tcacctgacg gccaccacgg agcaggagat cgttcgcatg ggtgcacgac tcggcgtttc
     2521 gaaaaagacg gcgattcacg cggcgcgtgt cgcccgtgat cggcagcggg agtactgtgg
     2581 acgatacgcc gcgatgggac gctcggtcct cgaggagatt ggcgatgagc ccgcgatcat
     2641 cgtcgcggga cgtccgtatg tgtacggatc gaacgaggcg aacctcgcgc ttccccgcaa
     2701 gctcatcagc cgtggctacc acgccatccc gatggacctg ctgccgccga tcggcgattc
     2761 ggtctacgag cgcaatgtgt ggcattacgg tcagcagatc ctcaacgcga tcgagcacgt
     2821 gaagcgtaaa cccagccttc acatctgcct ggtgtcgtgc ttttcctgct ttccggacgc
     2881 gagcatttat catctggtgc gcaccgagct gtcgggccac accttctgct atctcgaggt
     2941 cgattcgcac acggcgcatg cggggttcga gacgcgggtg ggcgcatttc tcgacatcat
     3001 cgaggaacgc gagcgggcga tgcgccaccg ggcggggagg acggcatgag catgatgatg
     3061 gaaggcccga cggcgccgcc ggtgctgcag gcgcgcttct ccgacgacag cacgcacatc
     3121 atcgacagcg atgggaatcg cgtcccgctc accgatccgc gcgtggtcct ggtctggacc
     3181 atcgactgca gcccgtatgt ccagcgcctc atcgtgagtt tcatgcgcca tcacggcatc
     3241 cgctgccgcg tgagcgccga taccggcaag gcgacgctcg agtacgcgaa gcgcatcagc
     3301 tccgggcgcg agtgcatcgc cttccgctcc atcgccggct ccatcattcg cgacctggcg
     3361 gagagccgga acccggacga gatcaccatc tactataacc acgatcagca cggcccctgc
     3421 cagaacgggg cctggccgga gatctggcgg atgtatcaac acgcaataaa cctgcgcaac
     3481 gtggtcctct tttcgaactt caatttccgc aacaactact ggggcctggg cgatgagctg
     3541 gggaacgagc tcatgacggc catcgtattc ggcgattgct tcgacgaggc cgagggcgtg
     3601 ttgcggtgta tcgcgaaaga cgccggcgct gcgctcgatg aattcaatcg cgctatcgat
     3661 acggttgttg acgcggccgc cggcgggatc gccccggcat tcaaggcctt caaggaatgg
     3721 tcgagacgga tggccgcgat tccgctctcg gccggcctcg atgagacgcc gcgcgttctc
     3781 atcttcgggg gcttgaatac ccggctggtg catcatccca tcacgacgta cttcatcgag
     3841 atcggggtga tcccgaaaat cgtggatttc ggagagatca ccatcgccaa cgagagcgaa
     3901 ccagcgctgc ggtataatta catgcgcggc agggtccggg cgcgcgacta tatgcggttc
     3961 ggtccggtcc tgttggattt cttccggccg tcgagcgatc gccgcgcggt atgggaggcg
     4021 ctgcgtgcgc ggcttgcggt cacgctcatc gagaagatgg tgcggcgata ccggcgcgtc
     4081 gcgcatactt cggggctcat ccacgatccg cacatcccgc acgaggaggt cgtctatgcc
     4141 ggcgaccggc tggtcacctc gatcagctac gcggaaaccg tcctcatcgc ggggcgatac
     4201 gtgaagacga tcgagagtgg cgcctacgac ggcctggtgc aggtcggggt cttcggctgc
     4261 ccgcccacca tcaacgcgca tgccgtgctg cggccgcttg ccaacaagag cgaggtgccg
     4321 tacgtggcgc tcgatgtcga ggggccgtgg atatcggcca accagaccgt gctcctggag
     4381 agcctcgcgg tgcaggcgcg gcgcgaacgg gcgcggaaaa tcgcggcgcg aactacccgg
     4441 ccttcgggac gttgatcgaa tcgagcccga gatcgagcag catccggttg cgcttctcat
     4501 cgttcggccc cttctcgatg accggactga gcacgtcggc gacgcagacc ggcaccacca
     4561 gcccattgcg gatcgtcgca tcggggcaag cggcgcagaa ttcgagctcc ccgttttcat
     4621 tgagccccgc gggctgctgg aagtaaaact ggttaaagct gatggtgttc ccgggcttca
     4681 tgagcatcca cagcagccgt gcggttgtgg cgattgtccg aggctggagg ctcatgagcg
     4741 ccgaaaggac catcgcgaaa acaagcagcg ccttgctctc ggggaagatg gcgaaattgt
     4801 agcgtttctt gatcgcgtta tgcagtttgt ccagcgccat ctggaactta agatagttgt
     4861 acgaggcgct gatatactcg aaggtcttgt ccggacggat gatggagagc acgatataaa
     4921 agagccatcg tttcgcgttc gtgtccttgt tcgatgagta gtggaggagg cagtcgagcc
     4981 cgtagtgctg cttcatgtag gcctcgatgt cggcgcccag gacctcctcc tcgccgtgcg
     5041 atttgctggt tttgatgatg ttcttcgagc ttttgacgcg cttggagaat ttttcggaca
     5101 ggccgcgcgt gtcgctgtac agggtcacca ggacgcacgg tgcgtattcg gtggccagca
     5161 cgtaatcgac gacatcgcgc aactgggaaa gagtggactt gtagagcgtg aggctgagcg
     5221 caggcatgat cccgtttcgc ttgatgcgct tcaggatctc gtcgcgaatc gggtcgagct
     5281 ctttctccgt tctcgcgtga acggcgtcgg gacgcttctg atgcgaatcg acatggacat
     5341 ggatgaagtc cagcccggcg ttattatact ggcggagcag ttcatcggtc agggtgagcc
     5401 cgttggagag cacattgacc ttgacgccct tcgccttgat gtaacggatc acctcgtgta
     5461 gttccggatg aagtgtcggc tcgccgcccg cgagggtgac cgcctcgaga tttctttgag
     5521 taaaggcgaa atcgacctct tctttaataa gcgcaagtgg tttggtatag gttccacggt
     5581 ttttataaca tgccttgcag gacaggttgc atttttggtt tacttcgatg aggatatgcg
     5641 gcgtatcctt tcccttgaaa tggccaacga tggatttgga cataaaaaac tccttgcaca
     5701 taaagctgaa catacatcat ctatagtgca ttctgcgatc aaatgttttt taagaaacat
     5761 caatttttta ttaaagtctg actggtcggt tttcaataat tattttatta ttcgcttaat
     5821 attatttgca aatacaactt gctaattctt tgtgacggca ataattcatt cgagccgggc
     5881 gtattaattt tatttataaa ttcgatttct tcggtcaatt gtaacggcaa ttctatcaat
     5941 aatccaatgg actggcaatc gtattcgatc tggaggttcc catgctaaag cctgccgaca
     6001 tcatggacaa tgaacggtat gttgatatca aagaagagaa tctggaaaac ttttccccga
     6061 aggatttctt taaatttgcc gatgacccaa tagataaaaa gaccgcacag ttctcgaaat
     6121 acttcaatca catgctgacc gaagggttta tcatgcgcga ggtgcgcagc ccggccagca
     6181 aaaaggtgcg catttacgag cgttcttcga ggaagctgat cgatgtgctc atgcttggtt
     6241 ctaacaacta tctcgatttc gccaatgagc cctacattat aaataagacg atacaggcga
     6301 tatgcgagta cggaatcggc tgcggcggtc cgcccctcct caatggcacc acgtcgctgc
     6361 accgccgcct cgagggacgt ctcgcggcaa tgaaggggac cgaggacgcg atggtctact
     6421 cgtcggggta tgcggcgaat gtcggctggg cgaccggtct cctcaacgag ggtgattttc
     6481 tcgtctacga ctggcagagc catgcgagcc tgcacgacag cgtgaagatg gggaaattca
     6541 ccggcgtccg ttttttacac aacgacatgg aggacctgga ccttcaactg accaccgtgc
     6601 gccgcaacag tccgaaggga aacgtggtcg tgtgcgtgga gggcgtctac tctatggacg
     6661 gcgacatcgc gccgttgccg gagatcaagg cgctctgcaa aaaacaccgc gcgctcctgt
     6721 gcatcgacga cgcgcacggc accggcgtgc ttggcaaaaa cgggcacggc acccaggaac
     6781 attacgacat gatgggcgag gtggacatca tcatggggac cttcagcaag agcttcggtg
     6841 tcaccggcgg tttcatcgcc tcgtcaaaga agatcatcga ctacctgcgg tt
//
LOCUS       DAORHF010000200         6886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_10838_length_6886_cov_5.166447, whole genome shotgun sequence.
ACCESSION   DAORHF010000200 DAORHF010000000
VERSION     DAORHF010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_6886_cov_5.166447"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PKJ16_14195"
     CDS             complement(<1..329)
                     /locus_tag="PKJ16_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HNV48192.1"
                     /translation="MISGTTVSNKSIDVAGIGSALVDFTVSVDDATLARLGLEKGRMQ
                     LVDAAQSAKILAALAGLPLSRTPGGSAANTMAGVAALGGASFFMGTIGADEYGEFYIE
                     ATERAGVR"
     gene            535..738
                     /locus_tag="PKJ16_14200"
     CDS             535..738
                     /locus_tag="PKJ16_14200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48193.1"
                     /translation="MKTKKTIDRKAGAGTRVSKKRTETQIIKDLVKDIRLYAKTLSQN
                     DRERFKGKLIKFLNTLAKEAERL"
     gene            735..890
                     /locus_tag="PKJ16_14205"
     CDS             735..890
                     /locus_tag="PKJ16_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48194.1"
                     /translation="MTLDDCRKCKYHEVFQDGYVICTYLKFRDQRVIKSESDGTIVLV
                     ECPLEKK"
     gene            complement(906..1292)
                     /locus_tag="PKJ16_14210"
     CDS             complement(906..1292)
                     /locus_tag="PKJ16_14210"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HNV48195.1"
                     /translation="MTRTMRRCAVLIGIAGFTVVLAAQLACARDVPDRSIVLKKIQAC
                     ALVVDVRTPAEFAAWHVAGAINIPLDQIEKRIGEFGDRNRPIVVYCRTANRSGVAKKI
                     LQRYGFTDVTNGGGLRDMPPIERTRC"
     gene            1448..2026
                     /locus_tag="PKJ16_14215"
     CDS             1448..2026
                     /locus_tag="PKJ16_14215"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HNV48196.1"
                     /translation="MISRIDHVSIAVRDYDRAAAFFTTLFGAVEGAGAEDPGMGFFWQ
                     LFSMGDLSRLELITPRGGKSYLDGFLKGREGGVHHITLETPDIEHVVRILDEHGVPHF
                     GRHDYVGGVWKEVFIHPRDAFGVLIQIAEFRPDDWLGHGVKLPAGRRWEVRMADGACA
                     IDIAHPGGGKVTIGLSREEARRFAEDIIAATG"
     gene            complement(2093..2443)
                     /locus_tag="PKJ16_14220"
     CDS             complement(2093..2443)
                     /locus_tag="PKJ16_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNV48197.1"
                     /translation="MKKIIVLLVALAVFVVSCSSGTIIKSIPEGAKVYMNDEYKGVTP
                     YSHSDTAIVGTAFRLKLSKDGYEDFQTIMKKDEKFNTIACVGGVFCLVPFLWVFEYNP
                     ERTYEMKKATPGKK"
     gene            complement(2577..4316)
                     /locus_tag="PKJ16_14225"
     CDS             complement(2577..4316)
                     /locus_tag="PKJ16_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV48198.1"
                     /translation="MTGTLPRLITIVCALFALSALAPAPASAKSIIDESAFGQKGADV
                     FLYVDMKALSRYLGANGITMTEIFRGMQKGEAASTDAYDELFNLVLGEITEIMVVGTS
                     AKVESGSGVLVFIGMPKYRKEIDASLAKRPRQSVHGYTLYEFDKQKNVFLARVAGMMV
                     IGFKPSLAAYLGAKKSGTKTTSAARRSFVAGAKGKTFYMYNSVSRYIKKQMEGAMAKG
                     AMMAKGLDANVYVKALLNLRSVETAFTVGSDVSVRAELASRLPADAKRLEMVSHFAIV
                     GASIALSFSGMLPKSAGQQALSADQERIARLQAMFDRIETRRTKTGVAVSYQFTSDDR
                     ARLIVAIKEALKRNAEERARLAREKKVAGLFAAIEEGSLERVVSQLAAVGDPNVSHKN
                     STPLCVAADKGNRQIVEYLISKKADVNGKTADDQLTPLHLAAMRGHTEVVTVLIGAGA
                     DINAKSKSNATPLQVALKNGAAGVARVLLQKNPDVKNSDEEGETALHLAAAGSETALM
                     KLLMERGADVNALSKKGDTPLHRAAAAGRLENVKALVEAGANRKATNYDGLTPREEAL
                     KNNHQQVADFLGN"
     gene            4478..>6886
                     /locus_tag="PKJ16_14230"
     CDS             4478..>6886
                     /locus_tag="PKJ16_14230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV48199.1"
                     /translation="MDHGSGLYLNFFSVGMMITMVFHLVMALFFFGIPERSKATTNMA
                     IGFLLFFVFNLAYSIANSLYHPAAAYHRWFTVALILPIITHLTAFFLYYPQPVNRRVI
                     KAFFITEYTISILLTLAFCGASFALRKTFLFEGHYWDYDNDQIGKIVALVIMLYIAIC
                     IASFIWRTITVKTAERWVCLWMGIALVLASVVPSIANTLSREGAIDRGLYQITWVLFN
                     ILGFFAMGLVYINNTKERTSFIGKLIGISVVTFLLLLQFISYIALTDKDVAYDEVRRT
                     AAELVLAGKNRPADLTYIIRFSPETGSFENVDERYKSAIPTDGLGVDFLNALSWRRIA
                     GLPAENFVPAATAIIEGAHADFAGHRAFIAGRLAALPAGTADPAAWLLREIAGVARTV
                     QYRANKINQLPDETFREQVTKFLAAQRGAFAPFRAAIEARLAQSTNEGDELKTELMRL
                     LAPMKEPGARRYRTDATGRVHHVGYMINDETAGRMYEVGFSYAAYRRGIHPFGLKLAG
                     ILVVLILLIRYGFQFFFKGALITPLRELSHAVKEVNSGNLDVSIPIKIEDEIGYVSRT
                     FNTMVVSIRDMIQTVTNNSLEVKTVSTDLNASSGQLSDIARELAAIVEQTAAAYEQMS
                     SSFEASLNEIKTQLELSDFVKSDISRINTNSEQLAGRISHLTESINGAVEQVSVGEKT
                     MAKSVKAIEEVAAYLTSIEETINSINEVADKINLLALNAAIEAARAGEAGRGFSVVAD
                     EVNKLADQTTELVKGIQTTIIQQANRIGNEIQFISGTSGIFKDVQQKIMETRDVMSET
                     IKFTG"
BASE COUNT         1303 a         2246 c         2016 g         1321 t
ORIGIN      
        1 cggacgccgg cgcgctccgt ggcctcgatg tagaactccc cgtattcgtc ggcgccgatg
       61 gtgcccatga agaagctcgc gccgcccagc gccgcgaccc cggccatcgt attggccgcg
      121 ctcccgccgg gcgtccgcga aagcggcagc cccgcgagcg ccgcgaggat cttcgcgctc
      181 tgcgccgcgt cgacgagctg catgcggccc ttctcgagcc cgagccgcgc gagcgtcgcg
      241 tcgtccacgg acacggtgaa atcgaccagc gccgaaccga tgccggcgac gtcgatggat
      301 ttgttcgata cggttgttcc cgagatcatg ttcccttccc tattccgttt ttcatggcgc
      361 cggcccgggc tatcgacacg cgccccgccg cgaacactca cgccgagggt gatattattt
      421 tcaacaagaa aaaccgcgcg ccggcgatcg catgccggcg cgcgtccaaa aatggattga
      481 cagcgccgcg taccgccggt acgcatgatc cctcgaacgc acgcgaggag aacgatgaaa
      541 acgaaaaaaa ctattgaccg aaaggcgggc gccggcacaa gggtatccaa gaagcgaacc
      601 gagacgcaaa tcatcaagga cctcgtcaag gacatccgct tgtacgcgaa aacgctttca
      661 caaaacgacc gggagcggtt taagggaaag ctgattaaat ttttgaatac gctcgcgaag
      721 gaagcagaac ggctatgaca ctcgacgatt gcagaaaatg taagtaccac gaagtattcc
      781 aggacggcta cgtcatctgc acctacctga aattccgcga tcagcgggtc atcaagagcg
      841 aaagcgacgg caccatcgtg ctggtcgagt gcccgctcga gaagaagtaa ccccccgcgt
      901 cagcatcagc accgcgtccg ctctatcggc ggcatgtcac gaaggccgcc gccattggtc
      961 acatcggtga aaccgtaccg ctgaaggatt ttcttcgcga cgccgctgcg gttcgccgtg
     1021 cggcagtaca ccacgatcgg ccggttcctg tcgccgaact cgccgatgcg cttctcgatc
     1081 tggtcgagcg gaatgttgat ggcgcccgcg acgtgccagg ccgcaaactc cgcgggggtg
     1141 cgcacgtcca cgacgagcgc gcacgcctgt atcttcttga ggacgatcga ccggtcggga
     1201 acgtcccgcg cgcacgcgag ctgcgccgcg agcacgaccg taaacccggc aattccgatt
     1261 aataccgcac accgtctcat cgttcttgtc atgaccgtcc gctcctcttc cccgttctcc
     1321 cgcgcccaac cggcgcatgt tgcgttgagt atagcgcacg cgtccggccc gtcaaaaaat
     1381 cgtcttgacg atggccgcac gcacagcgat catcacggac gaatcacaca cccgggaggc
     1441 ccgccagatg atatcgcgca tcgaccacgt ttcaatcgcc gtgcgggact acgaccgcgc
     1501 ggccgcgttc tttacaacgc tcttcggcgc cgtggagggg gccggcgcgg aggaccccgg
     1561 catggggttc ttctggcagc tcttctcgat gggcgacctg tcgcgtctcg agctcatcac
     1621 gccgcgcggc gggaaaagct atctcgacgg ttttctcaaa ggacgcgagg gcggcgtcca
     1681 tcacatcacc ctggagacgc cggacattga gcacgtggtg cgcattctcg acgaacacgg
     1741 cgtcccccac ttcggcaggc atgactatgt cggcggcgtg tggaaggagg tgtttatcca
     1801 cccgcgcgac gccttcggcg tgctcatcca gatcgccgag ttccggccgg acgactggct
     1861 cggccatggc gtgaagctcc cggcgggacg ccgctgggag gtcaggatgg ccgacggcgc
     1921 gtgcgcgatc gacattgcgc atccgggagg cgggaaggtg acgattggct tgtcgcgaga
     1981 ggaggcgcgt cgtttcgcgg aggacatcat cgccgcaacg ggctaacgcc cgcccccttc
     2041 cggcgacgag cgcacggaag ggacacgacg cacatgccgg cgcctcggtg tattattttt
     2101 ttccgggggt cgcttttttc atttcgtagg ttcgctccgg gttatactcg aaaacccaca
     2161 gaaacgggac caggcaaaag accccgccca cgcacgcgat cgtattgaac ttttcgtctt
     2221 ttttcataat agtctggaaa tcctcatagc cgtccttcga caacttcaag cgaaacgcgg
     2281 tcccgacaat cgcggtatcg gaatgcgaat agggggtaac ccccttgtac tcgtcgttca
     2341 tgtacacctt cgcgccctcg ggaatggatt tgataatggt accgctggaa caactcacga
     2401 caaacacggc caacgccact aacagcacga tgatcttttt cactggcaca ctccttgtat
     2461 gttgatagtt cgcgattagc gccggagtgt taccggtcgc cgcttccctg tcaaatactt
     2521 atcacccgca gggcggtttt ttcactccaa tgggactgca cccgccctgg gctcgatcag
     2581 ttccccagga aatccgccac ctgctggtga ttgttcttga gcgcctcctc gcggggggtg
     2641 agcccgtcgt agttggtcgc cttccggttg gcccccgcct cgacgagcgc cttcacgttc
     2701 tcaagccggc ccgcggcggc cgcgcgatgc agcggcgtat cgcccttctt gctcagggcg
     2761 ttcacatcgg cgccccgctc catgagcagc ttcatcagcg cggtctcgct ccccgcggcg
     2821 gccaggtgca gcgcggtctc gccctcctcg tccgagttct tcacgtcggg attcttctgc
     2881 agcaggacgc gcgcaacacc cgcggccccg tttttgagcg ccacctgcag cggggtggcg
     2941 ttcgacttgc tcttcgcgtt gatgtcggcc cctgcgccaa taaggaccgt cacgacctcc
     3001 gtgtggcccc gcatcgcggc caggtgcaac ggcgtgagct ggtcgtccgc cgtcttgccg
     3061 ttcacgtcgg cctttttcga aatgagatac tcgacgatct gccggttccc cttgtcggcg
     3121 gcgacgcaca gcggcgtcga gttcttgtgc gaaacgttgg ggtcccccac cgcggccagc
     3181 tgggagacga cgcgctcaag cgatccctcc tcgatcgccg caaagagacc ggcgaccttc
     3241 ttctcccgcg cgaggcgcgc ccgctcctcg gcgtttcgtt tcagcgcctc cttgatggcg
     3301 acgatgagcc gcgcgcggtc atcggaggtg aactgatagg agacggccac gccggttttg
     3361 gtgcgccggg tctcaatccg gtcgaacatc gcctggagcc gcgcgatgcg ctcctggtcg
     3421 gcgctcaagg cctgctgtcc ggccgatttc ggcagcatcc ccgaaaacga cagcgcgatg
     3481 ctcgcgccca cgatcgcgaa atggctcacc atctcgagcc gcttcgcgtc ggcggggagc
     3541 cgcgaggcga gctcggcgcg aacggacaca tcggagccca ccgtgaaggc cgtctcgacg
     3601 gaacgcagat tgagcagcgc cttcacgtag acgttggcgt cgagcccctt ggccatcatg
     3661 gcgcccttcg ccatcgcccc ctccatctgc tttttgatgt agcgcgaaac ggagttgtac
     3721 atatagaacg ttttcccctt tgcgccggcg acgaaactcc ttcgggccgc ggaggtggtc
     3781 tttgttccgc tcttcttggc tccgagatag gcggcgaggg acggcttgaa gccgatcacc
     3841 atcatcccgg cgacgcgcgc gaggaacacg ttcttctgct tgtcgaactc gtagagcgtg
     3901 tagccgtgga cgctctgccg cggccgcttc gcgagggagg cgtcgatctc ctttcggtac
     3961 ttcggcatgc caatgaacac gagcaccccc gaaccggact cgaccttcgc gctggtgccc
     4021 accaccatga tctccgtaat ctcgcccagc acgagattga acagctcgtc gtaggcgtcg
     4081 gtcgatgcgg cctccccctt ttgcatcccg cggaagatct cggtcattgt gatgccgttc
     4141 gcgcccagat agcgcgagag cgccttcatg tcgacataga ggaacacgtc ggcccccttc
     4201 tggccgaagg ccgactcgtc gatgatcgat ttcgccgacg cgggcgccgg ggcgagcgcg
     4261 gacaacgcga agagtgcgca tacgatcgta atcaggcgcg gaagtgttcc cgtcatgtca
     4321 gttctccttg ggtctcgaaa gatggaatgg tcgaaggatg gtcgatatcg ggccggctgt
     4381 ccagaatttt attatttcgc atggtcgtat tttactggac agggaatcat tcctgttgta
     4441 cgataatcat aaaaagacca caaaatggag tgtccgcatg gatcacggaa gcggtctgta
     4501 cctgaatttc ttctccgtgg ggatgatgat caccatggta ttccatctgg tgatggccct
     4561 gttctttttc ggcatcccgg agcgctcgaa ggccaccacc aacatggcga tcggctttct
     4621 gctgttcttt gtcttcaatc tggcctactc cattgccaac agcctgtacc atcccgcggc
     4681 cgcctatcac cgctggttca ccgtggccct gatcctgccc attataaccc atttaaccgc
     4741 cttttttctg tactacccgc agccggtcaa ccgtcgcgtc atcaaggcgt tttttattac
     4801 cgagtacacc atctcgatcc tgctcacact cgccttttgc ggcgcctcgt tcgccctgcg
     4861 caagacgttc ctcttcgagg ggcattactg ggactacgac aatgaccaga ttgggaagat
     4921 cgtcgccctc gtgatcatgc tctatatcgc catttgcatc gcttccttta tctggcggac
     4981 aattaccgtc aagacggccg agcgctgggt atgcctgtgg atgggaatcg cgctcgtact
     5041 cgcgtcggtg gtcccctcca tcgcgaacac cctcagccgc gagggcgcga tcgaccgggg
     5101 actctatcag atcacctggg tcctgttcaa catcctcggc ttctttgcaa tgggattggt
     5161 gtacatcaac aacaccaagg agcggacctc gttcatcggg aagctcatcg gcatcagcgt
     5221 ggtcaccttt cttctgctgc tgcagtttat aagctacatc gcccttaccg acaaggacgt
     5281 ggcctacgac gaggtccgac ggaccgccgc cgagctggtg ctggccggga agaaccgccc
     5341 ggccgacctg acctacatta tccgcttctc acccgagacg ggctcgttcg agaatgtcga
     5401 tgagcgctac aagtccgcca tacccaccga tggcctgggc gtcgatttcc tgaacgcgct
     5461 gtcctggcga cgcatcgccg ggcttccggc ggaaaacttc gtccccgcgg ccacggcgat
     5521 catcgagggc gcacacgccg atttcgccgg gcaccgcgcc ttcatcgccg ggcgactcgc
     5581 ggcgcttccc gctggcacgg ccgatcccgc cgcatggctc ctgcgcgaga tcgccggcgt
     5641 ggcgcggacg gtccaatatc gggccaacaa gatcaatcag cttccggacg agacctttcg
     5701 cgagcaggtg acgaagttcc tcgccgcaca acgcggcgcc ttcgccccgt tccgcgctgc
     5761 gatcgaggcg cgcctcgcac aaagcaccaa cgagggcgac gagctcaaaa ccgagctcat
     5821 gcggctcctg gcccccatga aggagccggg cgcgcggcgg tatcggaccg acgccaccgg
     5881 ccgggtccat catgtgggct acatgatcaa cgacgagacc gcgggccgga tgtacgaggt
     5941 gggcttctcc tacgcggcct accgtcgggg aatacacccc ttcggcctga agctcgccgg
     6001 aattttggtc gtcctgatcc tcctcatccg ctacggcttc cagttcttct tcaagggcgc
     6061 gctgattacg ccgttgcgcg aactctcgca cgcggtgaag gaggtgaaca gcgggaacct
     6121 cgacgtgagc atcccgatca agatcgaaga cgagatcgga tacgtgtcgc gcaccttcaa
     6181 caccatggtg gtgtccatcc gggacatgat ccaaacggtg accaacaact ccctcgaggt
     6241 gaagaccgtg agcaccgacc tcaacgcatc gtcggggcag ctctcggaca tcgcgcgcga
     6301 gctcgcggcc atcgtcgagc agacggccgc cgcctacgag cagatgtcct cctccttcga
     6361 ggcgagcctc aacgagatca agacgcagct cgagctctcg gacttcgtga agagcgacat
     6421 ctcgcgcatc aacaccaaca gcgagcagct cgcggggcgc atctcgcatc tcaccgagag
     6481 catcaacggc gcggtggagc aggtgtccgt gggcgagaag accatggcca agtcggtcaa
     6541 ggcgatcgag gaggtcgcgg cctatctcac cagcatcgag gagacgatca actccatcaa
     6601 cgaggtggcc gacaagatca accttctggc cctaaacgcc gcgatcgagg cggcgcgcgc
     6661 gggcgaggcg ggacgcggtt tctccgtggt ggccgacgag gtgaacaagc tcgccgacca
     6721 gaccaccgag ctcgtgaagg gcatccagac caccatcatc cagcaggcca accggatcgg
     6781 caacgagatc cagttcatca gcggcacctc cggcatattc aaggacgtgc agcagaagat
     6841 catggaaacg cgggacgtga tgtccgagac catcaagttc accggc
//
LOCUS       DAORHF010000201         6726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11229_length_6726_cov_7.211513, whole genome shotgun sequence.
ACCESSION   DAORHF010000201 DAORHF010000000
VERSION     DAORHF010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11229_length_6726_cov_7.211513"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..546
                     /locus_tag="PKJ16_14235"
     CDS             169..546
                     /locus_tag="PKJ16_14235"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48200.1"
                     /translation="MPSGIFRYEVRERTLVLTISTETVDIYNVGTLLERTDKAFVELD
                     FDSVIIDVTGLKRMDNSAAGALMVAMDRMKKRRSGGAVVVVTDNPAYYKLKHLYQYPL
                     DLFPTVDEAMAFLRDSSPSAKNE"
     gene            complement(655..1944)
                     /locus_tag="PKJ16_14240"
     CDS             complement(655..1944)
                     /locus_tag="PKJ16_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48201.1"
                     /translation="MPVFLFVVWVSGVAAIAVYQLVSAIRRNRRFTRCFKEVADAYGL
                     TFIRGSLFFFPKAYGSHNGFSIIVEKTTEGKQECLRIKVAHVRALDLDLYIRKESLGS
                     RLMNAVTRCDIVTGDRALDEMLYFHASRPAHVAASMNFETRKRLEKLSRQWETVSIKQ
                     DVMFVTRPLDRAVTASMIVECVDSLMAIGAAFADDGPLRARLMRNIERDPEPEVRIAN
                     LRALAAAGALDDEARSVLRRSCDDANAVVRFEAARLLGEEGFAHCEALLASPGSIDDA
                     LMAEILRYLRDRGRRVPADALMACYRGAALLATQLEVLAAMRDAADPRLCAFLVERLA
                     NDDSDIIIAAMQALETCGDISAVEKIYAKGKASLNPFVRSAANRAIAQIQSRLGKADK
                     GWLSLTETDGTDGALSVADGAGEGAVSVEVSKKNRKG"
     gene            complement(1950..2768)
                     /locus_tag="PKJ16_14245"
     CDS             complement(1950..2768)
                     /locus_tag="PKJ16_14245"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HNV48202.1"
                     /translation="MAADTNNLYASMTHRTAMVLGSAAMERLAAARVIVVGVGGVGSW
                     CAEALVRSGLVHLALVDSDIICPTNLNRQLQATSRNLGESKVREIRERLLLINPAADI
                     VAHHRVFDETTADDFDLAAFDYVIDAIDSIANKALLIERCVAAGVTVFCSMGAAAKTD
                     PTRVRVAPLSKTHGDPLARVVRRRLRARGVGLDVSCVFSDEPAADPAIETICGTGGCE
                     CVHDRDAWNESTGEEAADWCAMKKRVNGALVHVTGVFGFTLASMVIRDIMRRAE"
     gene            complement(2802..3866)
                     /locus_tag="PKJ16_14250"
     CDS             complement(2802..3866)
                     /locus_tag="PKJ16_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48203.1"
                     /translation="MFTSCLSAPVDFDEATWRKSIDETKPELLYAPHNHAGRFHNPWA
                     RMPETGFLSVLRWRLFTRASTVYTDEERAHLPAFVPDARARMAKHADRDYILWIGHNS
                     FAVKIGDTLYVTDPMLSNRAVIIKRKVPPAITAAELAAAAPRMVVIITHNHHDHLDLD
                     TVRALPESARFIVPRGVGELIRKYGKRDIVELDWWREAELGAGVRIACVPAQHWSRRI
                     DQGTNRSLWAGFVLIAPKTKILFAGDSGYFAGYREIGRKFPGIDYAFMPITAFHPRWF
                     MHYAHMNVDEALRAFDETGARYFIPTQWGTFPLGEEPVGYAALALKRAIARRGLDPSR
                     FVVMDIGGIEPVRSSRHKTN"
     gene            4239..5537
                     /locus_tag="PKJ16_14255"
     CDS             4239..5537
                     /locus_tag="PKJ16_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015253472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HNV48204.1"
                     /translation="MSKIDAKLKIETLALHGGQEPDPATGSRAVPIYQTTSYQFRDAD
                     HAAELFGLKAFGNIYTRLMNPTTDVFEKRIAAIEGGVGALAVASGQSAITLAILNIAQ
                     AGDEIVSADNLYGGTYTLFHYTLKKFGITVRFVPSNDLDAFRKAITPKTKAVYAESIG
                     NPKLDVADLEGIAGIAHASGIPFILDNTVSPYLLRPFDHGVDIIVYSATKFIGGHGTS
                     LGGVIVDSGTFDWTNGTFPLIADPDPSYHGLRFVEALAPIGNIAYILKARVTLLRDMG
                     PALSPFNSFLFLQGLETLHLRMPRHSENALAVARHLENHAAVDWVLYPGLASSANNAR
                     AKKYLPKGAGAILGFGIKGGTEAAKRFINALELVSHLANIGDAKTLAIHPASTTHQQL
                     SPEEQRATGVTPDFIRLSIGIEHIDDIIADIDQALAKASA"
     gene            5581..6549
                     /gene="cysK"
                     /locus_tag="PKJ16_14260"
     CDS             5581..6549
                     /gene="cysK"
                     /locus_tag="PKJ16_14260"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HNV48205.1"
                     /translation="MARIYDDNSTSIGNTPLVRINRIAAGLPGTILAKIEGRNPAYSV
                     KCRLGAALIWAAERDGILTPGRRDVTVVEPTSGNTGIALAYVCAARQYPLVLTMPETM
                     SIERRKMLKAFGAELILTEGAKGMPGAIAKAEEIVASDPKKHFMPQQFKNPANPEIHF
                     TTTGPEIWNDTDGSIDFFVAGVGTGGTITGVSRYLKQEKRAAVRSVAVEPADSPVLTA
                     LKAGATPKPGPHKIQGIGAGFKPDILDLDLVDEIATVTNDEAIEMARRLHLEEGITVG
                     ISSGAAMAAAVKIAARPESAGKTIVVILPDAGERYLSSALFEEIGA"
     gene            6546..>6726
                     /locus_tag="PKJ16_14265"
     CDS             6546..>6726
                     /locus_tag="PKJ16_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNV48206.1"
                     /translation="MKYDTKILHGAADRDPYTGALSIPIYPASTYHQKNIDEAPGYVY
                     SRAGNPTRRALEETLA"
BASE COUNT         1333 a         2179 c         2048 g         1166 t
ORIGIN      
        1 ggctctgacc cctgtcgctt ggggctctga cccctgtcgc ttggggctct gacccctgtc
       61 gcttggggct ctgacccttc ggatgtgaaa gcacgtatca aacggctatc attagacaca
      121 gccatggacg ataaaacgca cgaacgcaga tccaagccgc gtcccgatat gccatcggga
      181 atattccgct acgaggttcg ggaacgcacg ctcgtgctca ccatctcaac tgaaaccgta
      241 gacatctaca atgtggggac gctgctcgag aggaccgaca aggcgttcgt cgagctcgat
      301 ttcgattccg tcatcatcga cgtaaccggc ctaaagcgca tggacaactc ggcggcgggg
      361 gccctcatgg tcgccatgga ccgcatgaag aagcggcgat ccggcggcgc ggtggtcgtg
      421 gttacggaca atcccgccta ttacaagctc aagcatctct accagtatcc gctcgacctt
      481 ttcccgaccg tggacgaggc gatggcattt ttgcgggatt cctccccgag cgcaaaaaac
      541 gaataacaac gcaacaccct ctccacgctc gacgcgaggg agcgttgaca gtccatcgtt
      601 cttcattatg accaggttga ggttggattg gtgcgcgtga ttaaaaggag ttgtttatcc
      661 cttccggttt tttttcgaca cctcgacgct caccgccccc tccccggcgc cgtcggcgac
      721 gctgagggcg ccgtcggtcc cgtccgtttc tgtgagcgag agccaccctt tgtccgcttt
      781 gcccaggcgc gactggatct gcgcgatggc gcgattggcg gcgctgcgca cgaacggatt
      841 gagcgaggcc tttcctttcg catagatctt ctccaccgcg gaaatatcgc cgcaagtctc
      901 gagggcctgc atcgcggcga tgatgatgtc ggaatcgtcg ttcgcaagcc gctcgacgag
      961 aaacgcgcac agccgcggat cggcggcatc gcgcatcgcg gcaaggacct cgagttgcgt
     1021 cgcgaggagc gcggcgcccc gatagcacgc catgagcgcg tcagcgggaa cgcgccggcc
     1081 gcggtctcga agataccgca ggatctccgc catcagcgca tcgtcgatgg aaccgggcga
     1141 tgcgaggagc gcctcgcagt gcgcgaagcc ctcctcgccc agcagcctcg cggcctcgaa
     1201 gcgcacgacg gcattcgcgt cgtcgcacga tcgacgcagc accgagcgcg cctcgtcgtc
     1261 gagcgcccct gcggcggcca gcgcgcgcag gtttgcgatc cgcacctccg gttcggggtc
     1321 gcgctcgatg tttcgcatca gacgcgcgcg aaggggccca tcgtcggcga acgccgcgcc
     1381 gatcgccatg agcgaatcca cgcactcgac aatcatcgac gccgtgaccg cgcgatcgag
     1441 cggccgcgtg acgaacatca cgtcctgttt aatggacacg gtctcccatt gccgtgaaag
     1501 tttttccagg cgtttccgcg tttcgaaatt catcgaggcg gccacatgcg ccggccggga
     1561 tgcgtgaaaa tacagcatct cgtcgagcgc gcgatcgccg gttacgatat cgcaccgggt
     1621 cacggcgttc atcaggcgcg agccgagcga ctccttgcgg atgtacaggt ccagatcgag
     1681 cgcgcggacg tgcgccactt ttatgcgcag gcactcctgc tttccttccg tcgttttctc
     1741 gacgataatg gaaaagccgt tatgcgatcc atatgccttc ggaaagaaaa acaggcttcc
     1801 ccggatgaag gtaagcccgt acgcatcggc aacctccttg aagcaacgag taaaccggcg
     1861 gtttcgccgt atcgccgaaa caagctgata cacggcgatc gccgcgacac ccgaaaccca
     1921 cactacaaac agaaaaacgg gcatgacgct cactcggccc gccgcatgat gtcgcgtatc
     1981 accatcgacg cgagcgtaaa cccgaacacg cccgtgacgt gcaccagcgc gccgttcacg
     2041 cgcttcttca tcgcgcacca gtcggccgcc tcttcgccgg tggactcgtt ccaggcgtcg
     2101 cggtcgtgca cgcactcgca accgccggtg ccgcagatgg tctcgatcgc cggatcagcg
     2161 gccggctcgt cgctgaaaac gcaggaaacg tcgagcccga cgccgcgcgc gcgaaggcgg
     2221 cgccggacga cgcgcgcaag cggatcacca tgcgtctttg aaagcggcgc aaccctgacg
     2281 cgcgtgggat cggttttcgc ggcggccccc atggagcaaa agaccgtcac gcccgccgcg
     2341 acgcagcgct cgatgaggag cgccttgttc gcgatgctgt cgatggcgtc gatgacgtaa
     2401 tcgaacgcgg ccagatcgaa atcatcggcc gtggtctcgt cgaagacgcg gtgatgcgcg
     2461 acgatgtccg cggcgggatt gatcaagagc agccgttcgc ggatctcccg caccttcgat
     2521 tctccgaggt tgcgcgacgt tgcctgcaac tggcgattga ggttggtggg gcagatgatg
     2581 tcggaatcga cgagcgcgag atggacaagg ccggagcgca ccagcgcctc cgcgcaccag
     2641 ctcccgacgc cgcccacgcc gacaacgatc acccgcgccg ccgccaggcg ctccatcgcc
     2701 gcgctcccga ggaccatggc ggtccggtgc gtcatcgatg cgtagaggtt gttcgtatcg
     2761 gcggccatat caacgaagga gaccgtgacc ggacggtcct gtcaattcgt tttatgccgg
     2821 gacgagcgga cgggctcgat gccgccgatg tccatcacca cgaatcgcga cgggtcgagc
     2881 ccccgtcggg caatggcacg cttgagcgcg agcgcggcgt accccaccgg ctcctcgccg
     2941 agcggaaagg ttccccattg cgtgggaata aagtagcgcg cgccggtctc atcgaacgcg
     3001 cgcagcgcct catcgacatt catgtgcgca taatgcatga accagcgcgg gtggaacgcg
     3061 gtgatgggca tgaaggcgta gtcgatcccc gggaacttcc ggccgatctc acggtagccc
     3121 gcgaaatagc cgctgtcgcc cgcgaagagg attttcgtct tcggggcgat gaggacgaac
     3181 cccgcccaca gcgaacggtt ggtcccctgg tcgatgcggc gcgaccaatg ctgcgccggg
     3241 acgcatgcga tccgcacgcc cgcgcccagc tcggcctccc gccaccagtc cagctcgacg
     3301 atgtcgcgct tcccgtattt tcgaatcagc tcgccaacgc cacgcggaac gataaaacgc
     3361 gcgctctccg gcagggcgcg aacggtgtcg agatccagat ggtcgtggtg attgtgcgtg
     3421 atgatgacga ccatccgcgg cgcggccgca gcgagctccg cggccgtgat cgccggaggc
     3481 accttccgct tgatgatgac ggcccggttc gaaagcatcg ggtcggtcac gtacagcgta
     3541 tcgccgatct tcaccgcgaa gctgttgtgc ccgatccaga ggatatagtc ccgatcggcg
     3601 tgcttcgcca tccgtgcgcg cgcatccgga acgaaggccg gaagatgggc ccgctcctcg
     3661 tcggtataga ccgtgcttgc gcgcgtgaac agccgccaac gcaggacgga gagaaagccc
     3721 gtctcgggca tgcgcgccca gggattgtgg aagcggccgg cgtgattgtg cggagcgtag
     3781 agcagttcgg gtttggtttc atcgatactc ttgcgccagg ttgcctcatc gaaatcaacc
     3841 ggagcggaga ggcaggatgt gaacactgct agtgatagtg tcactaaaat tggggtcaga
     3901 gccctcttgg ctataccttt catcatcatt ctatcgatat acaacctact ttttctgggt
     3961 gaattcagga tgccagtgta ctacatcatc gtcatacaaa ctgatttttt tggggctctg
     4021 accctcttgg agctacatcc gggaaatcta cataggctct gacccccctt tttcacacaa
     4081 aagtaaacac caaggcacta ctgtaaccat tttcacatcg gatacttttt cccgcatttc
     4141 ggaaaaaatt cacttttctc ttgacaaaaa caatgtgcaa tatttattta ctactaattc
     4201 gctagtattg gtagtatttc atcagaccgg aggaaacaat gagtaaaatc gacgcgaaat
     4261 tgaagatcga aacgctggcg ctccacggcg gccaggagcc cgatccggcg accgggtcgc
     4321 gcgcggtccc gatctatcaa acgacctcgt accagttccg ggacgccgac cacgcggccg
     4381 agttgttcgg actgaaggcg tttggaaaca tctacacccg gctgatgaat cccaccaccg
     4441 acgtcttcga gaagcgcatc gcggcgatcg agggcggcgt cggggcgctt gccgtcgcga
     4501 gcggtcaatc cgccatcacc ctggccattc tcaatatcgc ccaggcaggc gacgagatcg
     4561 tttccgccga caacctctac ggcgggacct acaccctgtt ccactacaca ctgaagaagt
     4621 tcggcatcac ggtgcgcttc gtgccgtcga acgacctcga tgcgtttcgg aaggccatca
     4681 cgccgaagac gaaggcggtg tacgccgaat ccatcggcaa tcccaagctg gacgtggccg
     4741 atctcgaagg gatcgccggg atcgcgcatg cgagcggcat cccgttcatt ctggacaata
     4801 cggtttcgcc gtacctgctg cgccccttcg accacggcgt ggacatcatc gtctactcgg
     4861 ccacgaagtt catcggcggc cacggcacgt cgctgggcgg ggtgattgtc gattcgggaa
     4921 cgttcgattg gaccaacggc acattcccgc tcatcgccga tccggacccg agctatcacg
     4981 gcctgaggtt cgtcgaggcc ctcgcgccga tcggcaacat cgcctacatc ctaaaggcgc
     5041 gcgtgacgct gcttcgcgac atggggccgg cgctctcgcc gttcaactcg ttcctcttcc
     5101 tgcagggact ggagacgctg catctgcgca tgccgcggca ctccgagaac gcgctggccg
     5161 tcgcgcgcca tctcgaaaac cacgcggcgg tcgattgggt tctctatccc ggtctcgcct
     5221 ccagcgcgaa caatgcgcgt gcgaagaagt atcttccgaa gggcgccggg gcgatcctgg
     5281 gcttcggcat caagggcggg accgaggcgg cgaagcgctt tatcaacgcg ctcgagctgg
     5341 tgtcgcacct ggcgaacatc ggcgacgcga aaacgctcgc gattcacccg gcgagcacca
     5401 cgcaccagca gctctcgccg gaggagcagc gcgcgaccgg cgtcacgccc gacttcatcc
     5461 ggctgtcgat cgggatcgag cacatcgacg acatcatcgc cgacatcgac caggcgctcg
     5521 cgaaggcatc cgcgtaaatc gcgggtgcgg catcatcaca cacgatacga ggtgacgaac
     5581 atggcacgca tatacgacga caacagcaca agcatcggaa acacgccgct ggtccgcatc
     5641 aaccggatcg ccgcggggct gccgggaacg atcctggcga agatcgaggg acgcaatccc
     5701 gcctactcgg tcaagtgccg gctcggcgcc gcgctgatat gggcggccga gcgcgacggc
     5761 atcctcacgc cgggacggcg cgacgtgacg gtcgtcgagc ccacgagcgg caacaccggg
     5821 atcgcgctcg cgtacgtgtg cgccgcgcgg cagtacccgc tcgtcctcac catgccggag
     5881 accatgtcca tcgagcggcg caagatgctc aaggccttcg gcgccgagct catcctgacc
     5941 gagggcgcga agggcatgcc cggcgcgatt gctaaagccg aggagatcgt cgcgagcgat
     6001 ccgaagaagc atttcatgcc gcagcagttc aagaacccgg ccaacccgga gatccacttc
     6061 acgaccaccg ggcccgagat atggaacgac accgacggat cgatcgactt cttcgtcgcc
     6121 ggcgtgggga ccggcggcac catcaccggc gtgagccgtt atctgaaaca ggagaagcgc
     6181 gcggcggtcc gatccgtggc cgtggagccc gcggactcgc cggtgctcac cgcgctcaag
     6241 gcgggagcaa cgccgaagcc cggcccccac aagatccagg ggatcggcgc gggcttcaag
     6301 ccggacatcc ttgacctcga cctcgtggac gagatcgcca cggtaacgaa cgacgaggcg
     6361 atcgagatgg cgcggcgatt gcacctcgag gagggaatca ccgtcgggat ctccagcggc
     6421 gcggccatgg cagccgcggt gaaaatcgcg gcgcggcccg agagcgcggg aaagaccatc
     6481 gtggtgatcc tccccgacgc cggggagcgc tatctctcga gcgcgctctt cgaggagatc
     6541 ggcgcatgaa gtacgacacg aagatcctgc acggcgcggc cgaccgcgat ccgtacaccg
     6601 gggcgctcag cattcccata taccctgcgt cgacctatca ccagaagaac atcgacgagg
     6661 cgccggggta cgtgtactcg cgcgcgggca atcctacgcg ccgcgcgctc gaggagacgc
     6721 tcgcca
//
LOCUS       DAORHF010000202         6690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11333_length_6690_cov_4.402261, whole genome shotgun sequence.
ACCESSION   DAORHF010000202 DAORHF010000000
VERSION     DAORHF010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11333_length_6690_cov_4.402261"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="PKJ16_14270"
     CDS             complement(<1..1435)
                     /locus_tag="PKJ16_14270"
                     /inference="COORDINATES: protein motif:HMM:NF040105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV48207.1"
                     /translation="MRTKQERQTVKMYSAAEELLQKDEFFIVHEDEPKPVPAATHIAQ
                     GAEAPKTQFVTTDPTERKKGSEPLTGSRSSLDKDKRMDIPTIVSYLQWHMDRISENVV
                     GRGEIVRQAMYAIITREHMLLLSRTGMAKSFLANYIFNTFEQVRVFSSQASKDQTPDN
                     YFGPYNIDEFRKGRIRHNIHGSIIEADLVFLDEFFDASDVVLRSLLSVLNERRFINGQ
                     EVIDAAVHTAIATANYMRMNEVTEAVLDRFNFKSIIPEDNNTYTQLLIDHTYAYSHGK
                     PIDPEKKIPFGNMIYLSEIVKNENHDIHVVVPDFIYFMKNVIVNKFVSNMRKSEYNFF
                     ISPRKQAKMTDFLRASAILDNRFEVTMKDLENLYLTVCTLNSYVSVKTKDKSEKDVYL
                     DAYQQTMVHFNSTGAIQQIELLLGIRKFFQDLRENPEKKERLHEAKGLLEGLKGLIRK
                     LFPSRVKDDDGDMTIEQLKNTVLELNPV"
     gene            complement(1455..3065)
                     /locus_tag="PKJ16_14275"
     CDS             complement(1455..3065)
                     /locus_tag="PKJ16_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48208.1"
                     /translation="MERNELADLSKDELVKRIEEMQKMMQGASCWRSDLEVDYQYIIE
                     NSADIIFILDKEGKLVYKNKAGEDFFPIRREDAIGMHYSKYIPALELERAKVVFAAVL
                     NEGKELRNERMKSMDTQNRTVYFIANFSPIRSCDGDIIGLHGILRNITDLHDTEKKLR
                     ENTRRLEEKIREQVQQAEELRRMTVINEEMVNNAPIAIAMMDPAGIMLSENAALKQIM
                     GHKPNETRIGTNLSQYQGWVDAGLQGILDEAVKQRRPVRLDNVHYIPISRDRELILNA
                     RMNPIMERDGTVKSVLAMLEDITEQSKYASKMQRAEKLSAMGLLAAGVAYELKVPINL
                     MAIDLNFISKNIDESSPVRDYLKSLKDELDRIKKITEQLLNLSKPEQDEIELIEVNKL
                     LQSHPIQISLSRLQKNGLTVNMKLPDRSPVIRGTQSQVVQVLLHLISNAEDAMPDKGE
                     LTITVGQVESGGVKFAQILVADTGIGIPEENLKRIFQPFFSTKGHKSTGLGLMVTYSI
                     IDNLGGTIGVKSIPGEGTAMRILIPAVE"
     gene            complement(3075..3467)
                     /locus_tag="PKJ16_14280"
     CDS             complement(3075..3467)
                     /locus_tag="PKJ16_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48209.1"
                     /translation="MDNLKKALYLLLVFLVLGTATGLALMIFVGNSQSFGFLTGTALS
                     TILSFLWLIGARIGLKKNALVLLGITLGGFPLRLVILAVFAFGGLYLFKMNSTFFAIA
                     FLAGTIYSLVVEVWFLNTVSVPGRKKLQ"
     gene            complement(4839..5915)
                     /locus_tag="PKJ16_14285"
     CDS             complement(4839..5915)
                     /locus_tag="PKJ16_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HNV48210.1"
                     /translation="MTTKTIHITLGRMGDRILMRFPYNTEVIAVMRGIPGCSWDRSNR
                     AWSAPYSAERLDQIEQALSSYTLINDETMPDSEQRRLEALQRLERELIIRRYSRKTIA
                     SYRHYNLELLRFTGKRPDGITNDDIKNYLRDLAESRGMAVSTLNSAINALKFHYGTVR
                     GTDFIYDIARPAKDKKLPIVLSADEVRRIIGAIANLKHRTIIMLIYSAGLRLGEAITL
                     TVHDIDFTRKTIHIRGAKGRKDRYSLLSDAIVDTLRAYLRSYPHARWLFEGQQRGRHV
                     TDRTVQKVFETACRRIKLGKNATVHTLRHSFATHLLEKGIDLRYIQELLGHASCKTTE
                     IYTHVSNRRLNAIKSPLDDIINGP"
     gene            6067..>6690
                     /locus_tag="PKJ16_14290"
     CDS             6067..>6690
                     /locus_tag="PKJ16_14290"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV48211.1"
                     /translation="MRMRADTTWRWMIAGALACVVAVCAGTIAARGDGSYRWPLGVAT
                     GLTGTLGEWRAVHLHQGIDISTLTRNGLPVLAAGDGRLVRLMSWRRGYGNALILDHGG
                     GILTLYGHLGGFEESRHGLGALVPVVKALYNADDITLEFSGREHRYRRGERIADSGES
                     GSGVSHLHFEFRDGRGTPNPLALMPVRDADAPIITDVFVCALAGGALA"
BASE COUNT         1294 a         1799 c         2037 g         1560 t
ORIGIN      
        1 cgacgggatt gagctccagg acggtgtttt tcagctgctc gatcgtcatg tcgccatcgt
       61 cgtccttcac ccgggacggg aagagcttgc gaatgaggcc cttgagcccc tcgagaaggc
      121 ccttcgcctc gtggagtcgc tccttcttct cggggttctc ccgcagatcc tggaagaact
      181 tccggatccc cagcagaagc tcgatctgct ggatggcgcc ggtgctgttg aagtgcacca
      241 tcgtctgctg ataggcgtcc aggtacacgt ccttctcgga cttgtccttg gtcttcaccg
      301 agacgtagct gttgagcgtg cagacggtga gatacaggtt ttcgaggtcc ttcatggtca
      361 cctcgaaccg gttgtccagg atggcggagg cgcgcaggaa gtcggtcatc ttcgcctgct
      421 tgcgcgggct gatgaagaag ttgtactcgc tcttgcgcat gttgctcacg aacttgttga
      481 cgatcacgtt cttcatgaag tagatgaagt cgggcaccac cacgtggatg tcgtggtttt
      541 cgttcttcac gatctccgag aggtagatca tgttgccgaa gggtatcttc ttttcggggt
      601 cgatgggctt cccgtggctg taggcgtacg tgtggtcgat gagcagctgc gtataggtgt
      661 tattgtcctc ggggatgatc gacttgaagt tgaaccggtc gagcaccgcc tcggtcacct
      721 cgttcatgcg catgtagttg gccgtcgcga tggccgtgtg cactgccgcg tcgatcacct
      781 cctggccgtt gatgaagcgc cgctcgttga gcaccgacag gagcgagcgc agcaccacgt
      841 cgctggcgtc gaagaactcg tccaggaaca ccaggtcggc ctcgatgatg gagccgtgga
      901 tattgtgccg gatgcgcccc ttgcggaact cgtcgatgtt gtacggcccg aaataattgt
      961 ccggcgtctg gtccttgctg gcctgcgagg agaacacgcg cacctgctcg aaggtgttga
     1021 agatatagtt cgccaggaac gatttcgcca tgcccgtgcg cgacaacagg agcatgtgct
     1081 cgcgcgtgat gatggcgtac atcgcctggc ggacgatctc cccccgtccc acgacgttct
     1141 cggagatgcg gtccatgtgc cactggaggt agctcacgat ggtgggaatg tccatccgct
     1201 tgtccttgtc gagcgacgag cgagaacccg tgagcggctc gctccctttt ttgcgctcgg
     1261 tcgggtcggt ggtcacgaac tgggtcttgg gggcctcggc gccctgcgcg atgtgcgtcg
     1321 ccgcggggac gggtttcggc tcgtcctcgt ggacgatgaa gaattcgtct ttttggagca
     1381 actcctcggc ggcgctgtac atcttcacgg tctggcgttc ctgttttgtc cgcatggcgc
     1441 tctccttgat gtcgttattc caccgccggg atgaggatcc gcatggcggt tccctcgccg
     1501 gggatgctct tcacgccgat cgttccgccc aggttgtcga tgatcgaata ggtcaccatg
     1561 agccccaggc cggtggactt gtgccccttg gtgctgaaga acggctggaa tatcctcttg
     1621 aggttttcct ccggaatccc gatcccggtg tcggccacca ggatctgcgc gaactttacg
     1681 ccgccggatt cgacctgtcc caccgtgatc gtaagctcgc ccttgtccgg catcgcgtcc
     1741 tcggcgttcg agatcaggtg caagagcacc tggaccacct ggctctgcgt tcccctgatc
     1801 acggggctgc ggtccgggag cttcatgttc accgtgagcc cgtttttctg aagacggctc
     1861 aggctgatct gtatcgggtg gctctgcagg agcttgttca cctcgatgag ctcgatctcg
     1921 tcctgctcgg gcttcgagag attgagcagc tgttcggtga tcttcttgat gcggtcgagc
     1981 tcgtccttga gcgatttcag gtaatcgcgc accgggctcg actcgtcgat gttcttggag
     2041 atgaagttaa ggtcgatggc catgaggttg atgggcacct tgagctcgta ggccaccccc
     2101 gccgccagaa gccccatggc ggagagcttc tcggcgcgct gcatcttgct ggcgtatttc
     2161 gactgctcgg tgatgtcctc gagcatcgcc agcacgctct tgacggtccc gtcgcgctcc
     2221 atgatcgggt tcatccgcgc gttgaggatc agctcgcggt cgcgcgagat cgggatgtag
     2281 tggacattgt ccaggcgcac cggccgccgc tgtttcacgg cctcgtccag aatgccctgc
     2341 aggccggcgt ccacccagcc ctggtattgc gagaggttgg tgccgattcg cgtctcattg
     2401 ggtttgtgcc ccatgatctg tttgagcgcc gcgttctccg agagcatgat gccggccggg
     2461 tccatcatgg cgatcgcgat gggcgcgttg ttcaccatct cctcgttgat gacggtcatg
     2521 cgcctgagtt cctcggcctg ctggacctgc tcgcggatct tctcctcgag gcggcgggtg
     2581 ttctcccgga gctttttctc cgtatcgtgg aggtcggtga tgttccgcag aatgccgtgc
     2641 agcccgatga tgtcgccgtc gcaggagcgt atgggcgaga agttcgcgat gaagtacacc
     2701 gtgcggttct gggtgtccat cgacttcatg cgttcgtttc gcagctcctt gccctcgttg
     2761 aggacggcgg cgaatacgac cttcgcccgt tcgagttcca gcgccggaat gtacttggag
     2821 taatgcatcc cgatggcgtc ttcgcggcga atggggaaga aatcctcgcc cgccttgttc
     2881 ttgtatacga gcttgccttc cttgtcgagg atgaagatga tgtcggcgga gttttcgatg
     2941 atgtattggt agtcgacctc caggtccgag cgccagcacg aggcgccctg catcatcttt
     3001 tgcatctcct cgatgcgctt gaccagttcg tccttgctga ggtccgcgag ttcgtttcgt
     3061 tccatagacc gcccctactg gagttttttg cgtcccggca cgctcacggt gttcaggaac
     3121 cacacctcga caaccaggct gtagatggtg ccggcgagaa aggcgatggc gaagaaggtc
     3181 gagttcatct tgaaaagata caggccgccg aaggcaaata cggctagaat gacgagccgg
     3241 agggggaatc cgcccagagt gatgcccaaa agcaccaggg cgttcttttt taggccgatg
     3301 cgggccccga taagccacag aaaagaaagg atcgtgctca acgccgttcc cgtaaggaac
     3361 ccaaagcttt gggagttgcc gacgaatatc atgagcgcga ggccggtggc ggttccgaga
     3421 accaggaaaa caagcagaag atataaggct tttttcaggt tgtccattgc agacccgtac
     3481 agatttgtct ttgaatgaat acaacggcag tgctggtgta ataattgaag ctatccgggg
     3541 gcggatattt ggtcaacaat attttacctg atcgagggtc gtctggaggg gctccgtttg
     3601 cgtgcgccgg cttgaaatga tgcattggcg aaggaacaat ccgcgatcag gcgtttcgat
     3661 ggcgtttcga tgggcctaag ctgctgatgg ggtggggata tatgtgaaat atcccgcagg
     3721 gcatccaatt tttgagataa tggccgatct aggcgcgttt ggcgcgatcc atcgggatga
     3781 tgggtaaacg aacatgcgtg attctgtgct cgcgcacccg cactgcgagg gagcacgaag
     3841 acgctcgaat ccgcttcatg gccgtatgga tgcctgcaga ttcagcatga gcccgcatgt
     3901 ggcgcgatat aatggcgcat cgcgccgttc gagtcgcgtt ttatctcgag aaaatcgttg
     3961 tgatcacccc aaacggtcga tgatcgcatc acgtggcgct attgatgtcg atgaaaacac
     4021 cgaaaacagc gattcgtgac cctgaaaatc ttttcatact catcatgacg accgattgat
     4081 gcatggtata gcggttaaat gatgaaaaaa ttggatcatg tatctcattt tcttgttcac
     4141 ggagcaattt tcagcgtgaa aagggctcga gaggcatgcc tgaacgagtt tttcgatgct
     4201 tgatgatcga ttacaatgaa tttttgcgtt cgatttactc ttcagtcggc atgatgatca
     4261 tcattcgatc gggaaatcgc gaaggacgcg gcgccaatgg gctcaatgga ggcgtagtaa
     4321 agcataatga agcgctggat cctcgatgtg agcgcgatca tcatgcatga aaattctttt
     4381 tacgcatctc acgcgcgatg atctcgcatc gcacgcgtac gcatcggatg attttcattc
     4441 ttcatgatga catgaagatg cgaatgaaat gattgcgcgt gagaagggaa aaacaaaaaa
     4501 tgaaaaaaaa tttttaaaaa atacaaatag ttgttgaaaa aaaaatcaat agtaaacgat
     4561 taaatgaaaa agtggagtta taaaaaaaca atttcaggag aagaacaatg gcgaccaaaa
     4621 aaaccaccaa gaagaagacc gtaaagaaag cagcgaagaa gaccacgaag aaggctgcaa
     4681 agaagaaaaa gaaatagcac tacgccttct gtatttcaaa aaggcggact gcagtccgcc
     4741 tttttaattg gcgcgatgca tgcgccgttg ttttcccttc cgaacgtaaa aaagtcctca
     4801 acacaagcca atttccacta caccactgca tcgagcgcct acgggccatt tatgatgtca
     4861 tcaagcgggc ttttgatcgc gttgagcctt cgattgctca catgcgtgta gatttccgtc
     4921 gttttgcagc tcgcatgccc taacagctcc tgaatatacc gcagatcgat gcctttctcg
     4981 agcaaatgag tcgcgaaact gtggcgaagg gtgtgcaccg tcgcgttttt cccgagtttg
     5041 atcctccgac aggcggtctc gaacaccttt tgcacggtgc gatcggtcac gtgacgaccg
     5101 cgttgttgac cctcgaagag ccatcgcgca tgcggatagg agcgcaggta cgcgcgcagc
     5161 gtatcgacaa tcgcgtcgga caacagggaa tagcggtcct tgcgcccctt ggcgccccga
     5221 atgtggatcg ttttgcgcgt aaaatcgata tcgtggacgg tgagcgtgat cgcttcgccg
     5281 agccgcaggc ccgccgagta gatgagcatg atgatggtgc ggtgcttgag gttcgcgatg
     5341 gcgccgatga tgcgccgcac ctcgtcggcg ctgaggacga tgggcagctt tttgtccttt
     5401 gcggggcgcg cgatgtcgta gatgaaatcc gtcccgcgga cggtcccgta atggaacttg
     5461 agcgcgttga tggcgctatt gagcgtcgaa acggccattc cgcggctctc ggccaggtcg
     5521 cgcaggtaat tcttgatgtc gtcgttggtg atcccgtcgg ggcgttttcc ggtgaaccgc
     5581 agcagttcga ggttgtaatg ccgataggag gcgatcgtct ttcggctgta tcttcttata
     5641 atgagctccc gttccaaccg ctgcagggct tcgaggcgtc gttgttccga atcgggcatg
     5701 gtctcgtcat tgatgagagt gtaggacgat agagcttgct cgatctggtc aagcctctcg
     5761 gcggaatagg gggcgctcca ggcgcggttc gagcggtccc atgagcaacc gggaatcccc
     5821 cgcatgacgg cgattacttc cgtattgtac ggaaaccgca tcagaatacg atcgcccatg
     5881 cgccccaggg ttatgtggat ggttttggtg gtcatatcgg ccgcctgtat gcaatgattg
     5941 atgcgctcta cccgtactac ggccccaaag gctggaaatt ggaccatttt tgcctccgac
     6001 cggcgttatg gcgtcgcaga atgggggaaa catcttcttg acatgccgcg gaagcgtggc
     6061 cctgatatgc gcatgcgtgc ggatacgaca tggcggtgga tgatcgcggg ggcgcttgca
     6121 tgcgtcgtcg cggtatgcgc ggggacgatc gcggcgcgcg gggacggctc ctaccggtgg
     6181 ccgctcgggg tcgcgaccgg actcaccggg accctggggg agtggcgcgc ggtgcatctt
     6241 catcagggga tcgacatcag caccctgacg cgcaatggcc tcccggtgct ggcggccggt
     6301 gacggccggc tcgttcgact gatgtcgtgg cgacgcggct acgggaacgc gctcatcctg
     6361 gaccatggcg gcggcatcct gacgctctac gggcatcttg gcggcttcga ggagtcgcgg
     6421 cacggccttg gggccctggt gccggtcgtg aaggcgctct acaatgcgga cgatatcacg
     6481 ctcgaatttt ccggtcgcga gcaccgctat cgtcgcggcg agcgcattgc cgacagcggc
     6541 gagtcgggat cgggggtgtc gcacctgcac ttcgagttcc gggacggtcg cggcacgccc
     6601 aatccgctcg ccctcatgcc cgtgcgcgac gccgacgcgc cgattatcac cgatgtgttt
     6661 gtgtgcgcgc ttgccggtgg cgccctggcc
//
LOCUS       DAORHF010000203         6662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11402_length_6662_cov_4.715302, whole genome shotgun sequence.
ACCESSION   DAORHF010000203 DAORHF010000000
VERSION     DAORHF010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11402_length_6662_cov_4.715302"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="PKJ16_14295"
     CDS             <1..352
                     /locus_tag="PKJ16_14295"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNV48212.1"
                     /translation="EAGQVADEQIARTDLSETLVDLRTRLAAKKKLLADLYKVFDNAA
                     LPQTLEVEKAVGGVIVEIEELKGKIAYYRDRIDLPEITVHLTAGPRGAVTGSPGSGWE
                     WIRTLGIESIMMRY"
     gene            356..859
                     /locus_tag="PKJ16_14300"
     CDS             356..859
                     /locus_tag="PKJ16_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48213.1"
                     /translation="MATHNDIRHPMFRLKRGVRAIACIAMCSMALISCATIDFPTPQG
                     FAKFTKDQRDYRAISADGVRIKARRIKNEPYGDLRMWQDAIGHHFESLGYKEKESGVL
                     AATEGYSGRFGEYRAVFNGREHRYIAALFVGKGDIVIIEAAGEEAVFAKRRDAVMTSI
                     RSFNPGK"
     gene            complement(843..1445)
                     /gene="yihA"
                     /locus_tag="PKJ16_14305"
     CDS             complement(843..1445)
                     /gene="yihA"
                     /locus_tag="PKJ16_14305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HNV48214.1"
                     /translation="MKITKAVFVKSSMNPKGYPSYDHPEIAFLGRSNVGKSSLINMLV
                     GSASLVKVGSRPGVTTAVNFFLVNDAVSIADLPGFGYAKVPKSVKQAFVPMIRDYCAH
                     RRNLRIAFLLIDVRRTPDDLERDIITLLSDKGIPVALTITKCDKVSKSALRTRMKEIA
                     EALMVETDQVFPTSSKTKSGKRELLRLIGDYCLRGATSPD"
     gene            complement(1442..1900)
                     /gene="rimI"
                     /locus_tag="PKJ16_14310"
     CDS             complement(1442..1900)
                     /gene="rimI"
                     /locus_tag="PKJ16_14310"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HNV48215.1"
                     /translation="MTTNHDTARIRHATVGDIDSIAEIERRSFAGSWSAVMIAEALDR
                     PESSMLCACADDRIVGYALYRHVLDEAELLRIAVSEEARRSGHGALLVGAIIDECRSR
                     GVATIHLEVNGANHAAIALYARCGFAEAGRRKNYYGAGEHALLMVRDTAL"
     gene            2051..2269
                     /locus_tag="PKJ16_14315"
     CDS             2051..2269
                     /locus_tag="PKJ16_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HNV48216.1"
                     /translation="MGKKAHTVIVRSDLCKGCELCIHFCKRSVLRFSPEFNIQGYHFA
                     EADPAAECNGCMVCTSVCPEVAIEVHYE"
     gene            2262..3344
                     /locus_tag="PKJ16_14320"
     CDS             2262..3344
                     /locus_tag="PKJ16_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HNV48217.1"
                     /translation="MSKVFMSGNHTFAEAAIRAGCRCYFGYPITPQNELGEYMAANMA
                     NVGGVFIQSESETAAINMVIGAASTGARAMTSSSSPGVSLKQEGISFLAGFELPAVIV
                     NVMRGGPGLGNIAPAQGDYFQATRGGGHGDYRTPVFAPSSVQEAADLTAIAFDVADRY
                     RTPVMILADGMLGQIREPVVFPDPIGELPEKEWALKGRGDGPSRYMASLILDALEMEK
                     HNWKLVRKYEEIAKNEVRCEQFMLDDAEIVAIAYGTTARIAKGAINRLRKDNIPVGLL
                     RPISLWPFPKNEIIDLTKRVRKFIVFEMSTGQMLEDVLLAVQGRAEVQFHGRPGGVVP
                     TPIEFSRILYREYQILQKGRNGASHS"
     gene            3362..4123
                     /locus_tag="PKJ16_14325"
     CDS             3362..4123
                     /locus_tag="PKJ16_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV48218.1"
                     /translation="MTIKNPWPIYYKKDVITHYCPGCGHGIVHRIVAEVLEELDVGRR
                     AICVNPAGCAVLAYQYFDIDMLESAHGRGTAMATGIKRSRPDLFVFTYQGDGDLSAIG
                     TAETIHSANRGEQICVIFINNAVYGMTGGQMAPTTLIDMKTTTTPKGRTARVDGFPLH
                     ITDVVATFPGVAYAERVAVNGPAGINRTKKAITTAFQTQIDGLGYSIIEVLSPCPTNW
                     KLNSIDSFAWITDVMAKEFPIGVLVDRRRKQADAD"
     gene            4113..4664
                     /locus_tag="PKJ16_14330"
     CDS             4113..4664
                     /locus_tag="PKJ16_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV48219.1"
                     /translation="MLIKMIFAGSGGQGVLTAGNILGNAAMIDDYHVTYLPSYGAAMR
                     GGTANCTICISDEEIASPVASSPDFVVSLNKPSTVKFANRLESGGQLIYNSSLIDTFP
                     YRGDIDLYPVPANAIAARLGNERSANMVALGAFIKIVRVIKLETVEKSIAQLMGSKAK
                     LVETSCTALRAGFDGFPFSNGQA"
     gene            4758..5186
                     /locus_tag="PKJ16_14335"
     CDS             4758..5186
                     /locus_tag="PKJ16_14335"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNV48220.1"
                     /translation="MPITQVSVAVQNTPGKLNEICEILEKENINIKAIMASSVLSPVQ
                     VHMIVDEPAKAENVLRGSGLTVSTREVVAVATPDHPGGLNAVLRPLREAKVNIECLYP
                     FITLHGDEAIIIIEVDKIKEAKEVLKKHWVKAYGAELYKR"
     gene            complement(5391..6170)
                     /locus_tag="PKJ16_14340"
     CDS             complement(5391..6170)
                     /locus_tag="PKJ16_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HNV48221.1"
                     /translation="MEKKCGYTVDEHGIALMEINNPPMNALSTPVLADIRETVIKAIN
                     DDSVRVVVFTGAGKAFIAGADITEVKAQNRAVDGSTYLENGHDLFNLIENAEKPFIAA
                     INGFCLGGGMEFALACHIRLADETAQLGLPEIKLGIIPGYGGTQRTPRYIGKGRALEL
                     VLSGNFITGKTAETYGLVNRATPKGQVVDEAKQLAKAIASRSRLAVRAAMKAVREGME
                     MDMLPAQKFERELFGTLCETDDKKEGVAAFLEKREPAFKDK"
     gene            6315..>6662
                     /locus_tag="PKJ16_14345"
     CDS             6315..>6662
                     /locus_tag="PKJ16_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48222.1"
                     /translation="MKLRTTILTAVTLSLFVMAICCGLAAQTPPGRTAQKTKPQTDAP
                     RPSMLLADIAKNVESYKRKTVTMPLRLKHVDRVFEKIAFYDTKNVDIVFDYASIKRDP
                     QFQSNFATIREGMM"
BASE COUNT         1410 a         1975 c         1984 g         1293 t
ORIGIN      
        1 cgaggcgggg caggtcgccg acgagcagat agcccgcacg gacctctcgg agacgctcgt
       61 ggacctgcgt acgcggcttg cggcgaagaa gaagctcctg gccgatctct acaaggtatt
      121 cgacaatgcc gcgctcccgc agacgctcga ggtcgagaaa gcggtcggcg gcgtcatcgt
      181 ggagatcgag gaactcaagg ggaagatcgc atattatcgc gaccgtatcg accttccgga
      241 aataacggtt cacctgaccg ccggtccgcg cggtgccgtc accggaagcc ccgggagcgg
      301 ttgggaatgg atcaggacat tgggaattga aagcatcatg atgcggtatt aacccatggc
      361 aacgcacaac gacattcgcc atccgatgtt ccgcctgaaa aggggcgttc gtgcgatcgc
      421 gtgtatcgcc atgtgctcga tggcgctcat ctcatgcgcg accatcgatt tccccacgcc
      481 gcagggattc gccaaattca ccaaagacca acgcgactac cgcgccatct cggccgatgg
      541 cgtgcggatc aaggcgcgcc gcatcaagaa cgagccctac ggcgacctgc ggatgtggca
      601 ggacgcgatc ggccatcact tcgaatcgct cggatacaaa gagaaggagt cgggggttct
      661 cgcggcaacc gagggatata gcggacgttt cggcgaatac cgggcggtct tcaacggtcg
      721 ggagcaccgc tatatcgcgg cgctgttcgt cggaaagggc gatatcgtca tcatcgaagc
      781 ggcgggagaa gaggcggtgt tcgcaaaacg ccgcgacgcc gtcatgacgt ccatccgctc
      841 gttcaatccg gggaagtagc cccgcgcagg cagtagtcgc cgatcagccg cagaagctcc
      901 cgcttcccgc ttttcgtctt cgacgaggtc ggaaaaacct gatcggtctc caccatgagc
      961 gcctcggcga tctccttcat ccgcgtgcgc agtgccgatt tcgacacctt gtcgcacttg
     1021 gtgatcgtca gggccaccgg aatccccttg tccgacagca gcgtgatgat gtcgcgttcg
     1081 agatcgtccg gcgttctccg cacatcgatg agcaaaaacg cgatgcgcag gtttcgacga
     1141 tgggcgcaat agtcccgaat catgggcacg aaggcctgct tgaccgactt gggcaccttc
     1201 gcgtatccga acccgggcag gtcggctatc gaaacggcat cgttgacgag gaagaaattg
     1261 accgccgttg tcacccccgg ccgggatcca acctttacca ggctggcgct tcccacaagc
     1321 atgttgatga gcgatgattt tcccacattg gatcgcccta aaaacgcgat ctcagggtgg
     1381 tcatacgacg ggtatccctt cgggttcatg ctgcttttca cgaatacggc cttggtaatc
     1441 ttcataacgc agtgtcccgc accataagca gcgcatgctc cccggcgccg tagtagtttt
     1501 ttcgccggcc ggcctccgcg aacccgcacc gagcgtacag ggcgatcgcc gcatggttgg
     1561 cgccgttcac ctcgagatgg atggtcgcga cgccccgcga gcgacactcg tcgatgatcg
     1621 ccccgacgag aagagcgcca tgaccactcc tgcgcgcctc ctcgctgacg gcgatgcgta
     1681 ggagctccgc ctcgtcgagc acatgccgat ataacgcata cccgacaatg cggtcgtcgg
     1741 cgcacgcgca cagcatgctc gattccggac ggtccagcgc ctcggcgatc atgacggcgc
     1801 tccaggaacc tgcgaatgag cgccgctcga tctcggcgat cgagtcgata tcgccgaccg
     1861 ttgcatgacg aatacgggcc gtgtcatgat tggtcgtcat gtcgaaaaaa acctttgaca
     1921 aatcgagtga taaaattttt ggtcacatca acatttcttc gctataccgt tatacgctgg
     1981 atgcgccgtt cgccccaaaa agcgatcatg gggctcacgg catacgctgt aaattacatt
     2041 ttggtgatca atgggaaaga aagcacatac ggtcatcgtt cgaagcgatc tctgcaaagg
     2101 gtgcgagctg tgcattcatt tctgtaaaag aagcgtcctt cgcttttcgc ctgagttcaa
     2161 catccagggc tatcatttcg ccgaggcgga ccccgcggcc gagtgcaacg gctgcatggt
     2221 gtgcaccagc gtatgtcccg aggtggccat cgaggtgcac tatgagtaag gtgttcatga
     2281 gcggcaatca cacctttgcc gaggcggcca tacgagcggg ctgccggtgc tatttcggct
     2341 atcccatcac cccgcagaac gagcttggcg agtacatggc cgcgaacatg gccaatgtgg
     2401 gcggcgtttt catccagtcg gaaagcgaga cggcggccat caacatggtc atcggcgcgg
     2461 cctcgaccgg cgcccgtgcg atgacgtcct cgtcgagtcc cggggtaagc ctcaagcagg
     2521 aggggatttc cttcctggcc ggattcgagc ttccggcggt catcgtcaat gtgatgcgcg
     2581 gcggccccgg actgggaaac atcgccccgg cccaggggga ctatttccag gccacccgcg
     2641 gcggcggaca tggcgactac cgcacgcccg tctttgcgcc gtcaagcgta caggaggcgg
     2701 ccgatctcac ggccatcgca ttcgacgtgg cggaccggta tcggactccg gtgatgatcc
     2761 tcgccgacgg catgctcggg cagatccgcg aaccggtggt gtttcccgat ccgattggcg
     2821 agcttccgga aaaggaatgg gcgctcaaag gacgcggtga cggccccagc cgatacatgg
     2881 cgtcgctcat tctcgacgcg ctcgagatgg aaaaacacaa ttggaagctc gtgcgcaagt
     2941 atgaagagat agcgaaaaac gaggtgcgct gcgagcagtt tatgcttgat gacgccgaga
     3001 tcgtggccat tgcgtacggg accacggccc gcatcgcgaa gggcgctatc aaccgcctgc
     3061 gcaaggacaa catcccggtg gggcttctgc ggccaatcag cctatggccg ttcccgaaaa
     3121 atgaaatcat cgatctcacc aaacgcgtga ggaaattcat cgtttttgaa atgagcacgg
     3181 gacagatgct cgaggacgtg ctcctggccg tccagggacg cgccgaggtg cagttccatg
     3241 gacgcccggg cggcgtggtc ccgaccccga tcgagttctc gcgcatcctg tatcgggagt
     3301 atcaaatact gcagaaggga cgaaacggcg cgtcacactc ataaacccgg acagaacacc
     3361 aatgacaata aagaacccct ggcccatata ctacaagaag gacgtcatca cgcattactg
     3421 cccggggtgc gggcatggca ttgtccatcg catcgtcgcc gaggtgctcg aggagctcga
     3481 tgtgggccgg cgggcgatct gcgtgaaccc ggccgggtgc gcggtgctcg cgtaccagta
     3541 tttcgacatc gacatgctcg aatcggccca cgggcgcggg acggcgatgg ccaccgggat
     3601 caaacgctcg cgtccggacc tcttcgtgtt cacctaccag ggcgacggcg atctctccgc
     3661 catcggcacc gccgaaacga tacactcggc caaccgtggc gagcagattt gcgtcatctt
     3721 catcaacaac gccgtgtacg gaatgaccgg cgggcagatg gcgccgacga cgctcatcga
     3781 catgaaaacg acgacgaccc cgaagggccg caccgcgcgc gtcgacggct tcccgctgca
     3841 catcaccgac gtggtcgcga cctttcccgg cgtggcctac gcggagcgcg tcgcggtgaa
     3901 cgggccggcg ggcatcaatc gcaccaagaa ggcgatcacg acggccttcc agacgcaaat
     3961 cgacggcctg gggtattcca tcatcgaggt cctctcgccc tgtcccacca actggaagct
     4021 caactcgatc gattccttcg cctggatcac cgacgtcatg gcgaaggagt tcccgatcgg
     4081 cgttctggtc gatcgcagga ggaaacaggc cgatgctgat taaaatgatc tttgccggat
     4141 cgggcgggca gggcgttctc accgccggga acatcctggg caacgccgcg atgatcgacg
     4201 actaccacgt gacctatctg ccgtcgtacg gggcggcgat gcgcggcggg acggcgaact
     4261 gcaccatctg catctcggac gaggagatcg cctcgccggt cgcctcatcg cccgatttcg
     4321 tcgtgtcgct caacaaaccc tccacggtga aattcgcgaa ccgcctcgaa tcgggcgggc
     4381 agctgattta caactcgagc ttaatcgata cgttccccta tcgcggcgat atcgatctct
     4441 atccggttcc ggcaaacgcg attgcggcga gactgggaaa cgaacgatcg gcgaacatgg
     4501 tcgcgttggg ggcgttcatt aaaatcgtgc gggtcatcaa gcttgaaacc gtcgagaaaa
     4561 gcattgccca gctgatgggc agcaaggcga aactggtcga aacgagctgc acggcgctca
     4621 gggccggatt cgacgggttc ccgttttcca acgggcaggc gtaacggcaa tcgcgtactc
     4681 gagtgacgat ggcgcgacga gggacgactc gccgcgagat gcgaatgtaa tccgacgaac
     4741 aaggagggga cccaccgatg cccatcaccc aggttagcgt ggccgttcag aacacgccag
     4801 gaaagctcaa cgaaatctgc gagatcctcg aaaaggagaa tatcaacatc aaggccatca
     4861 tggcctcatc ggtgctctcc ccggtgcagg tgcacatgat cgtggacgaa ccggcgaagg
     4921 ccgagaatgt gcttcgcggg agcggtttga cggtgtcgac tcgcgaggtg gtcgcggtcg
     4981 cgacgccgga tcatcccggc ggactcaatg ccgttctccg gccgctccgc gaggcgaaag
     5041 tgaacatcga gtgcctgtat cccttcatta cgctgcatgg cgatgaggcg atcatcatca
     5101 tcgaggtcga taaaattaaa gaggccaaag aggtccttaa aaagcattgg gtgaaggcct
     5161 acggggcgga gctgtacaag cgataaagct ttttggtttc gaacaaaccg aattccgaaa
     5221 cgtgcgttgc gttcgtatca taattccgtg agtattgcgc ggttgaaacg taatcggttg
     5281 ttttctcgtt tatatatatc atacgcgttc ccgatggaat ccgccggcgt ttcgataggc
     5341 gaccgccgat gaaataccac cgggaatagc cggtgtgtcg gaaataatcg ctacttgtcc
     5401 ttgaacgccg gctcgcgttt ctcgagaaag gccgcaaccc cttctttttt gtcgtccgtt
     5461 tcgcacaagg tcccgaaaag ctcgcgttcg aatttttgcg ccgggagcat gtccatctcc
     5521 attccctcgc ggaccgcctt catcgcggcg cgcaccgcga ggcggctccg tgacgcaata
     5581 gccttggcga gctgtttcgc ctcatcgaca acctgtccct tgggcgttgc gcggtttacc
     5641 aggccgtagg tttccgctgt tttccctgtg atgaaattcc cagagagcac cagctcgagc
     5701 gcgcgtccct ttccgatata ccgcggcgtg cgctgggtgc cgccgtaccc gggaatgatg
     5761 ccgagtttga tctcgggcag gccgagctgc gccgtctcgt cggccagacg gatatggcag
     5821 gcgagggcga actccatgcc gccgccgagg cagaatccgt tgatcgcggc gataaacggc
     5881 ttctcggcgt tctcgatcag gttgaacagg tcgtgcccgt tctcgaggta ggtgctgccg
     5941 tcgacggcgc ggttctgcgc cttcacctcg gtgatgtccg cgcccgcgat aaacgccttg
     6001 ccggcgccgg taaagaccac cacgcgcacc gaatcgtcgt tgatcgcttt aatgacggtt
     6061 tcgcggatgt cggcaagcac cggggtgctt agcgcattca tcggcggatt gttgatttcc
     6121 ataagcgcga tgccgtgttc atcgacggta tagccgcatt tcttttccat gcgatactcc
     6181 tttcaggtaa tagaatttca taaacggtag tatcgtgaag ggaaatctct ccgtcaaata
     6241 ctttctgctc gggtgaatag tatttgacac gggtacggaa tgcgaaaagg gtacggccac
     6301 gggggaggct gatgatgaag ctgcggacaa ccatactgac ggcggtgaca ctatcgttat
     6361 tcgtcatggc aatctgttgc ggtctcgccg ctcagacccc gcccgggcgc accgcgcaga
     6421 aaaccaaacc ccaaacggac gcgccacgcc cctcgatgct gttggccgat atcgcgaaga
     6481 atgtcgagtc gtacaagcgg aaaacggtca ccatgccgct tcgccttaag cacgtcgaca
     6541 gggtcttcga aaaaatcgcg ttttacgata cgaagaacgt cgatatcgtt ttcgattacg
     6601 cgtcaataaa acgcgatccg caattccagt cgaatttcgc gaccatccga gaggggatga
     6661 tg
//
LOCUS       DAORHF010000204         6622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11494_length_6622_cov_4.914421, whole genome shotgun sequence.
ACCESSION   DAORHF010000204 DAORHF010000000
VERSION     DAORHF010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11494_length_6622_cov_4.914421"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            469..999
                     /locus_tag="PKJ16_14350"
                     /pseudo
     CDS             469..999
                     /locus_tag="PKJ16_14350"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(1010..1555)
                     /gene="pyrR"
                     /locus_tag="PKJ16_14355"
     CDS             complement(1010..1555)
                     /gene="pyrR"
                     /locus_tag="PKJ16_14355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HNV48223.1"
                     /translation="MSEQIILTGRDIERIIADTAARVCADIGEDADSFGIVGIQTRGV
                     ELADRIRALIERDSGKRIKSGTLDITFYRDDLSTRGVLPVIKETRIEFDITDMTILLV
                     DDVIFSGRTTKAALETLMSFGRPRAIRFFVLIDRGNRELPIQPDYCGYKIETGPTDQV
                     RVLLAQTDGTGDSVVLQPNGA"
     gene            complement(1661..4993)
                     /gene="carB"
                     /locus_tag="PKJ16_14360"
     CDS             complement(1661..4993)
                     /gene="carB"
                     /locus_tag="PKJ16_14360"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV48224.1"
                     /translation="MPKDTSIRKILIIGSGPIVIGQACEFDYSGSQACKALREEGYEV
                     VLVNSNPATIMTDPDLADRTYIEPITPDVVEKIIERERPDAVLPTVGGQTALNVALAL
                     AESGVFEKYGVRLIGAGIESIKKAEDRELFKKAMVKLDLYVPESGLASTVDEALAVLE
                     RIKLPLIIRPAFTLGGTGGNIVYNREEFVELVRKGLDESPISQVLLEESVIGWKEYEL
                     EVMRDKKDNVVIICSIENFDPMGVHTGDSITVAPQQTLTDREYQAMRDAAIRIIREIG
                     VDTGGSNIQFAVDPADGRMMVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGLTLDE
                     IPNDITRETPASFEPTIDYVVVKVPRWAFEKFEGADPRLGPQMKSVGEAMAIGRTFKE
                     SLQKALRSLENDRYGLGADGRKDLAALCAGMDEGKRRDVIEKNLVNPMEGRIFYIKYA
                     LSLGWSVARINELTGIDPWFLEQLREIVLAEEAFVGDFRANGLSRENVTAMKRLGFSD
                     VQLGYLTSADALAEIDAKGPSHQREYSLTLKRRESDVRAFRFENGIVPGYRLVDTCGG
                     EFEARTPYYYSSYDDQDEARDSGRRTVMILGGGPNRIGQGIEFDYCCCHASFALRDMG
                     IESIMVNSNPETVSTDYDTSDRLYFEPLTLEDILHIYRREKPEGVIVQFGGQTPLRLA
                     KSLMENGVRILGTSPDSIDRAEDRERFADVVRKLDLLQPDNGIAFAQDEAIAVAERIG
                     YPVLVRPSYVLGGRAMSIIYDKSSLIEYINTAVQLSPEHPILVDRFLDDATEVDVDAI
                     CDGESVYVGAIMEHVELAGVHSGDSACVIPPLTIDKPMRDRIAETTEALAFELGVVGL
                     INIQFAIKDDDLYVLEVNPRASRTVPFVSKTKGVPLAKIAVRVMLGEKLRDFGLGRIK
                     SIPYISVKEAVLPFNKFPGVDTILSPEMKSTGEVMGVSRFFGEAFYKAELAAGDRLPV
                     AGTVLLSINRKSQPEILSEVRLLFESGFRLVATEGTAQYYADHGITCERVRKVSEGRP
                     NILDLVKNGELDLIINIPAGKEPKSDAYIIRQSAVRYHIPIVTSESAARAAIHGMIVV
                     GRTGVLSVRSIQEYHEDVKNSQ"
     gene            complement(5122..5784)
                     /locus_tag="PKJ16_14365"
     CDS             complement(5122..5784)
                     /locus_tag="PKJ16_14365"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV48225.1"
                     /translation="MNLKTEISVGLIFFVALAVLGYYTILMSEKIFDNRKDYYYITVL
                     FPDVEGLNRDDKVKINGVMSGKVDDIQLRSTNMVWVKLKMFNTFTLYSNYKIKIKSES
                     ALGGKYVAISPGVGFEKGREYAVVTTRESLEGAALGDPFGLISEFVEENRHDIREAIS
                     NLRMITEKINTGQGTLGKLVSRDTMHDNAEDLIKEIRETIEDSREQAPVTSFIRAALT
                     AF"
     gene            complement(5815..6561)
                     /locus_tag="PKJ16_14370"
     CDS             complement(5815..6561)
                     /locus_tag="PKJ16_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48226.1"
                     /translation="MIEFVDVYKYYNTRAVLKGVSMRIERGETFVIIGQSGTGKTVSL
                     RHIAGLVDPDGGDVIIDGVKMNGAKRRVKTALREKMGVLFQSGALINWMTVGDNIALP
                     LVEHRLHPPDEIEKIVAEKLHMLQLTDARDKMPADISGGMRKRAGLARSLVRNPEIIL
                     YDEPSSGLDPVMTSVIDQLIRRMQREYNITSVVVTHDMGSAYAIADRIAMLYQGEIIQ
                     CGTPDEIRNTENPIVRQFITGSLAGPIPVV"
BASE COUNT         1152 a         2143 c         2059 g         1268 t
ORIGIN      
        1 catcctcaca tcagtcacac cgtgtcctgg gatcggctgg cagaggcggg cgcgttgatg
       61 accagaggtg cacacacagg caagatagcc gtggagatcc cttgatcgaa tcgcgcttcg
      121 atgcggtgct cttcgacctg gcgggaacgc tgttcgacga tcgcgcgctg cgtgatgtcc
      181 accttgagca gttgacgttc gccgcggatg cagccggtgt ccgggcggac gatcgacagc
      241 tacgtgcggc gtacggtcag ggcatggcgg aagcgttcgc gatgatgggc ggccgcccgc
      301 actaccggca ccacgatctg ttcgccgaag ctttccgcgg gatggcacgt gcactcggaa
      361 gccaattgga cgagtgtgac gtccaggcgg cagtgaacca acagggcaga gctacagtcg
      421 aattcgccga gctccggccg ggagcaacac tcacgctgca gcggctgcgg gacctgctgc
      481 agcgagccca tcgtgcccgt caccgacagc gacgtccgcc gcatcgcgcg cgccacgggc
      541 cgtgcgcccg aatcgttcgt ccgcttttac tccgacgacg acatggattt cgccgccgag
      601 tccggcgtgt ggatccgctt cccggccggc gtgcgcgcca tggggctccg acgccgaaag
      661 aatcgctgcc tgttcctggg cgacgacatg cgttgcacga tatatcagca ccgcccgcgc
      721 acctgccgca ccttccccta ccagaccgat ctcgacgaga gcggcgcgct ccggcgcgtg
      781 acgatgaacc ccatcgtgaa atgccgccgg cgccgcgtgg acgattcgcc cctgacgaaa
      841 ctcgtccgcg acagccgcgc cgaggaccgc gaagacgacc ggtactatgc gcgcgtcgaa
      901 atctggaacg aatccgtcgg ggggaagaaa cgcatgcggg acttcctcgc gtttgtcgaa
      961 cggccggccg gcgcgcgagg aaaaaagagt aagcgataac gtcggagaat tacgcgccgt
     1021 tcggctggag taccaccgag tcccccgtcc cgtccgtctg ggcgagaagc acccggacct
     1081 ggtccgttgg ccccgtttcg atcttgtagc cgcagtaatc gggctggatg ggaagctcac
     1141 ggttcccgcg gtcgatgagc acgaaaaacc tgatggcccg cgggcgtccg aagctcatca
     1201 gcgtctccag cgccgccttg gtggtgcgcc cggagaagat cacgtcgtcc accagcagga
     1261 tcgtcatatc ggtaatatcg aactcgatgc gcgtctcctt gatgaccggc agcacccccc
     1321 gcgttgagag atcgtcgcgg taaaaggtga tatcgagcgt gccgctcttg atccgctttc
     1381 cggaatcgcg ttcgatgagc gcgcggatgc ggtcggcgag ctccaccccg cgcgtctgta
     1441 tgccgacgat cccgaacgag tcggcatcct cgccgatatc ggcgcaaacg cgcgcggcgg
     1501 tgtcggcgat gatccgctcg atgtcgcgtc cggttaaaat gatctgttcg ctcatgattg
     1561 ttgtccggca agcgccccgc ccccgcggaa accgacagtc cccaccggcc ccgttcgggc
     1621 gccgctcgta taggtgtatt gtcggcagcg gtgcggggaa ctattgactg tttttcacgt
     1681 cctcgtggta ctcctggatc gagcgcaccg acaggactcc cgtgcgcccc accacgatca
     1741 tgccgtggat cgccgcccgg gcggcggatt ccgaggtgac gatggggatg tgataccgga
     1801 ccgccgactg ccggatgata tacgcgtcgc tcttgggctc cttgccggcc gggatattga
     1861 taatgagatc gagctccccg tttttcacca gatccaggat gttcggccgc ccctcgctca
     1921 ccttgcggac gcgctcgcag gtgatgccgt ggtccgcgta atactgcgcc gtcccctcgg
     1981 tggccacgag ccggaagccg ctctcgaaca ggaggcgcac ctcgcttaat atctcgggct
     2041 gggatttccg gttgatggac aaaagaaccg tcccggccac cggcagccgg tcgccggcgg
     2101 cgagctccgc cttgtagaag gcctcgccga agaagcgcga cacgcccatc acctcgccgg
     2161 tcgatttcat ctcgggcgag aggatcgtgt ccaccccggg gaacttattg aagggaagca
     2221 ccgcctcctt cacgctgatg tatgggatcg atttgatcct gcccaggccg aaatcgcgaa
     2281 gcttctcccc cagcatcacg cgcaccgcga tcttcgccag cggcaccccc ttcgtcttgg
     2341 agacgaatgg gacggtgcgc gaggcgcgcg ggttcacctc gagaacgtaa agatcgtcgt
     2401 ccttgatcgc gaactgaatg ttgatgaggc ccaccacgcc gagctcgaag gcgagcgcct
     2461 cggtggtttc cgcgatgcgg tctcgcatgg gcttgtcgat ggtaagcggc gggatcacgc
     2521 aggcggagtc gcccgagtgc acgccggcga gctccacgtg ctccatgatg gccccgacgt
     2581 agacgctctc gccgtcgcag atcgcgtcca cgtcgacctc ggtcgcgtcg tcgaggaagc
     2641 ggtcaaccag gatggggtgc tcgggcgaga gctgcacggc ggtgttgatg tattcgatga
     2701 ggctggattt atcgtagatg atggacatgg cgcggccgcc cagcacgtac gacgggcgca
     2761 cgaggaccgg atagccgatc cgctcggcga ccgcgatcgc ctcgtcctgc gcgaaggcga
     2821 taccgttgtc gggctgcagg aggtccagct tccggaccac gtcggcgaag cgctcccggt
     2881 cctccgcgcg gtcgatcgaa tcgggcgagg tccccagtat gcgcacgccg ttctccatga
     2941 ggctcttcgc aaggcgcagc ggcgtctgac cgccgaactg cacgatcacc ccctccggct
     3001 tttcgcggcg gtagatgtgc aggatgtcct ccagggtgag cggctcgaag tagagccggt
     3061 cggacgtgtc gtagtccgtg gacaccgtct cgggattaga gttgaccatg atggactcga
     3121 ttcccatgtc acgcaacgcg aaggaggcgt gacagcagca gtaatcgaat tcgatcccct
     3181 gcccgatccg gttgggtccg ccgcccagga tcataaccgt ccgccggccg gagtcccggg
     3241 cctcgtcctg atcgtcgtag gacgagtagt agtacggcgt gcgggcctcg aactcgccgc
     3301 cgcaggtgtc cacgaggcga tagccgggaa cgatgccgtt ttcgaagcga aacgcgcgca
     3361 cgtccgactc ccggcgcttg agcgtgagcg aatactctcg ctggtgcgac ggccccttcg
     3421 cgtcgatctc cgcaagcgcg tccgcgctcg tcagatagcc gagctggaca tcggagaacc
     3481 ccaggcgctt catggccgtg acgttctccc gcgaaaggcc gtttgcccgg aagtcgccca
     3541 cgaacgcctc ctcggcgaga acgatctcgc gcagctgctc gaggaaccag gggtcgatcc
     3601 ccgtgagctc gttgatccgc gccacgctcc atccgagcga aagggcgtac ttgatgtaga
     3661 agatgcgccc ctccatggga ttcacgaggt tcttttcaat gacatcgcgc cgcttgccct
     3721 cgtccatccc ggcacatagc gcggcgaggt cctttcggcc gtcggcgccg agaccgtacc
     3781 ggtcgttctc gagcgaacgc agcgcctttt gcagcgactc cttgaacgtg cgcccgatcg
     3841 ccatcgcctc ccccacggac ttcatctggg gacccaggcg cggatcggcg ccctcgaact
     3901 tctcgaaggc ccagcgcggg accttcacca ccacgtagtc gatcgtcggc tcgaacgacg
     3961 cgggcgtttc gcgggtgatg tcgttgggga tctcgtcgag cgtcaggccg atcgcgagct
     4021 tcgcggcgat cttcgcgatc gggaagcccg tcgccttgga ggcgagcgcg gagctgcggc
     4081 tcacccgcgg gttcatctcg atgaccatca tccgcccgtc ggcggggtcc acggcgaact
     4141 ggatattgga gccgccggtg tccacgccga tctcgcggat gatccggatc gcggcgtcgc
     4201 gcatggcctg atactcgcgg tcggtgagcg tctgctgggg cgccacggtg atggaatcgc
     4261 cggtatgcac ccccatggga tcgaagttct cgatggagca gatgatgacc acgttgtcct
     4321 tcttgtcgcg catcacctcg agctcgtatt ccttccagcc gatcaccgac tcctcgagaa
     4381 gcacctgcga gatgggcgat tcatccagcc ccttccgcac gagctccacg aactcctcgc
     4441 ggttgtacac gatattgccg ccggtgccgc ccagcgtgaa cgcaggccgt atgatgagcg
     4501 gcagcttgat gcgctcgagc accgcgagcg cctcgtccac cgtcgaggcg agtcccgact
     4561 ccgggacgta gaggtccagc ttcaccatcg ccttcttgaa gagctcgcgg tcctcggcct
     4621 ttttgatcga ttcgatcccc gcgccgatca gccggacgcc gtacttctcg aagacgcccg
     4681 actccgcaag cgcgagcgcg acattgagcg ccgtttgacc gcccaccgtg ggcagcaccg
     4741 catcggggcg ctcgcgctcg atgatcttct ccacgacgtc gggggtaatg ggctcaatat
     4801 aggttcggtc ggcgaggtcc ggatcggtca tgatggtcgc gggattggag ttcaccagga
     4861 ccacctcata gccctcctcg cgcagcgcct tgcatgcctg cgatccggaa taatcgaact
     4921 cgcacgcctg tccaatcacg atggggccgg agccgatgat gaggatctta cggatgctgg
     4981 tgtccttggg cattcaaacc tcgcggggga gcggcaaatt gtgcgatggt gaccatgcct
     5041 cggaaagctg tcaatgacga aacggcgaat tatcgtatgt atagtgcgac taatgggaaa
     5101 tgttacatgc gcgtaacaaa cctagaacgc ggtgagcgcg gcgcggatga agctggtgac
     5161 cggggcctgc tcgcggctgt cctcgatcgt ctcgcggatt tccttgatga ggtcctcggc
     5221 attgtcatgc atggtgtcgc gcgagacgag cttgccgagc gtcccctgcc cggtgttgat
     5281 cttttcggtg atcatgcgga ggttgctgat cgcctcgcgg atatcgtgcc ggttctcctc
     5341 gacgaactcc gagatgagcc cgaagggatc acccagcgcc gccccctcga ggctctcccg
     5401 tgtggtcacg accgcgtact cgcgcccctt ctcgaaaccc accccgggcg agatcgccac
     5461 gtacttgccg ccgagcgcgc tctcgctttt tatctttatc ttgtagttcg agtagagggt
     5521 gaaggtgttg aacatcttga gcttcaccca caccatgttg gtgctgcgca gctggatgtc
     5581 gtccaccttg ccggacatca ccccgttgat cttcaccttg tcgtcgcgat tgagcccctc
     5641 cacgtcggga aagagcaccg tgatatagta gtagtccttt cggttgtcga agatcttctc
     5701 gctcatgagg atggtgtaat accccagcac cgcgagcgcg acgaagaaga tgagcccaac
     5761 ggagatttcg gttttcaggt tcattgcccg attcgtcgct cctttcccgc cgcgctacac
     5821 caccgggatg ggcccggcga ggctcccggt tatgaattgc cgcactatcg gattctccgt
     5881 gttgcgaatc tcgtccggcg tcccgcactg gatgatctcg ccctggtaga gcatcgcgat
     5941 ccggtcggcg atcgcatagg cgctccccat gtcgtgcgtc accaccaccg aggtgatgtt
     6001 gtactcgcgc tgcatgcgcc gaatgagctg gtcgatcacg ctggtcatca ccggatcgag
     6061 ccccgacgac ggctcgtcgt acaggatgat ctccgggttg cgcaccagcg accgcgcgag
     6121 cccggcgcgc ttgcgcatgc cgccgctgat gtcggcgggc atcttatcgc gggcatccgt
     6181 caattgcagc atgtgaagct tttcagcaac aattttttcg atttcgtcgg gcggatggag
     6241 ccggtgctcc accaggggca gcgcgatatt gtcgcccacc gtcatccagt tgatgagcgc
     6301 gcccgactgg aagagcaccc ccatcttctc cctgagcgcg gtcttgaccc gtcgcttcgc
     6361 gccgttcatc ttcacgccgt cgatgatcac gtccccgccg tcggggtcca ccaggcccgc
     6421 gatgtgccgc agcgaaaccg ttttccccgt ccccgactgg ccgatgatga cgaaggtctc
     6481 gccgcgctcg atgcgcatcg acacgccctt gaggaccgcg cgcgtattgt agtacttgta
     6541 gacgtcgaca aactctatca tcactccttt ccgtagaaca ccgcggtgat aaagtagccg
     6601 atgatgagca ccatgagaaa cg
//
LOCUS       DAORHF010000205         6587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11579_length_6587_cov_5.433864, whole genome shotgun sequence.
ACCESSION   DAORHF010000205 DAORHF010000000
VERSION     DAORHF010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11579_length_6587_cov_5.433864"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            329..1552
                     /locus_tag="PKJ16_14375"
     CDS             329..1552
                     /locus_tag="PKJ16_14375"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HNV48227.1"
                     /translation="MKTCTISEAIEDLRNGRMIILVDNEDRENEGDLVVAAEYCTPDI
                     VNFMVTHGRGLLCVAMEQERLNALGLRLMVPENSDKFCTAFTVSVDARHGTTTGISAS
                     DRSLTVRALIDERTAPDDLTRPGHVFPLAAKRGGVLVRAGHTESSVDLARLAGLRPAA
                     VICEILKEDGTMARRPDLDVFAERHGLKIATISDLIKYRQHKERLIRRVSSATLPTDY
                     GKFHIMAYETEVDDAMHVALVKGDVDGRENVLVRVHSECLTGDVFASRRCDCGSQLHR
                     AMEMIDNEGAGVVLYMRQEGRGIGLGNKLKAYHLQDQGVDTVEANIMLGFPPDLRDYG
                     IGAQILVDLGLHTIRLITNNPKKVIGLEGYGLEIVERVPIEIAPVDENLRYLKTKKEK
                     MGHLILDEKGTGGPR"
     gene            1549..2475
                     /locus_tag="PKJ16_14380"
     CDS             1549..2475
                     /locus_tag="PKJ16_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48228.1"
                     /translation="MNAPETPTVVVVDDDEAIRYALQKKLTRFGLKIVSLDKAEDALF
                     LLKNPESAVDLVVTDIKLRKMDGIELLRHINILERPVPVLIITGQGNIEDAIRALRYG
                     ARDFIRKPFDINEVASIVRRILRSEQEKQLASAFGKYIRSERRSYVIPVDPGVIDVMS
                     YELTRNLMPFGLCNYTASENVALALQEAISNAMFHGNLEIDSAVRAERGIKGFNEEIE
                     ARRTDERYRDRTVRIQYDLNPEYVEYVIEDDGPGFNYNALPDPRDQQNFFKDSGRGIL
                     IIRIHMDEISWSERGNAIRMRKYRIDAKSAQH"
     gene            2497..3528
                     /gene="mtnA"
                     /locus_tag="PKJ16_14385"
     CDS             2497..3528
                     /gene="mtnA"
                     /locus_tag="PKJ16_14385"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HNV48229.1"
                     /translation="MYYTVKWNGDAVSMIDQRELPGRVVYRTYTNYHDVAASITDMVT
                     RGAPAIGITAAMGIALAAKTLEVTDAADLKTQLAAVCELFAKTRPTAVNLFWAIDRMK
                     AIIAANDSVDGIRAALEKEAVRILEDDVAMNKKIGANGAKYIADGDVVMTHCNAGALA
                     TGGYGTALGVIRAAVEAGKKIEVFACETRPYLQGARLTAWELQQDNIKVTLITDNMAG
                     HFMRAGVIKKIVVGADRIAANGDAANKIGTYTHSVVAKAHGIPFYVAAPTSTLDPATE
                     TGDAIVIEERGIDEVAYIQGVQIAPEGIFIRNPAFDVTPAENITAIITEKGNIESPKG
                     TGIRGLVGA"
     gene            3654..4223
                     /locus_tag="PKJ16_14390"
     CDS             3654..4223
                     /locus_tag="PKJ16_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48230.1"
                     /translation="MKRSSVVIALVCLAAFVATGCKPSAKNEIQYWENAKKDFAEAVS
                     SYPAFKDVLAAKMTEAQKLWDDAQKATGEEQAKQMKAANEKLNELLNQFTQIKHKSKG
                     VQDAIDSLNAKKLTVSEDATRKKAVAAARKALGEVKDLLANAKVAAEDEAKKITGEAI
                     SKLISAQGDIDRAIKSLEPKKSSTAKKKK"
     gene            4389..5645
                     /locus_tag="PKJ16_14395"
     CDS             4389..5645
                     /locus_tag="PKJ16_14395"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HNV48231.1"
                     /translation="MKKTIYSLLLLAVVLISSVPARSGQERLRVAVNSFDNLNNDKKY
                     AHLSAGFSEALTQGLFQIKNILVVERQGLDRIMREHELAQAGAIDEKSAVKTGRLMGA
                     NYLVMGKYQIAGGNVRVFCRLVNVETGAVSRDHSFQVTGSLDGIFTVQDELVKRITAS
                     FGISPGTDERRRVETVNRELSLTKDEFKLYLQARKEFYLATPDSYQRAIILYAKALGI
                     NGKFALAWAGLATANLYKGYERSWNGQSYDEEYFKALAAARQAIRFAPDLSETNCAMA
                     LVHAYWLPRPKRKEAEEWARKALAINENNAEAYFAISKARGGDLEYLKKTIEKDPQYI
                     LAYNWLGLVYQKMNMIDKAIEMHRTSIRLNPKWALEYANLAYCLFLQKKYPEAMENIN
                     RALQLKPDLKPAHVIKGMIVKASGGR"
     gene            complement(5651..6424)
                     /locus_tag="PKJ16_14400"
     CDS             complement(5651..6424)
                     /locus_tag="PKJ16_14400"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HNV48232.1"
                     /translation="MAHGAILWAVVFFLGAAWGSFFYTLALRWADGSFRESPWRALAT
                     PSRCPACASRIPLLRLIPALGYLIARGRCNSCGVRIPAAYPLAELGYGALAVVVALRL
                     GVNAPGAVLFLVMGLAIAIAIIDAKTMEIPLPLTLAFVALSIYPIILTGSVRDTLLGF
                     GIAAAFFVVMLLVFPGSFGGGDIKLAAAMGLLLGFELTIVMIETALVTGSVAGVIYAY
                     RTKKGLRVRFPFAPFLAAGLVVALLFGRDIVLLYYRIMF"
BASE COUNT         1393 a         2025 c         2165 g         1004 t
ORIGIN      
        1 gccgatgtga acgccctgtc gaagttcgac aagaccgcgc tcgactacgc cgagaaggcg
       61 ggcaaggcgg aggtcgtggc gtatcttgcg acgctcaacg cgaagaaggg gccggggaag
      121 aagtgagacg cgcggcgctc cgatatgccg cctgcgcata gagcacatca aggatcacat
      181 ggcgatcctg cgcgatgtat tgatgggtcg gccccgtgta tttgagagga tggcgatgcc
      241 gcattaatat cttgacgctg aaatccacgc atttttaatg caaaaaaaat gggatacgac
      301 gtttcgccgc atcaatcggg tggccgctat gaaaacctgc accatatctg aagccatcga
      361 ggatctccgc aatggccgga tgatcatcct cgtggacaac gaggaccgcg agaacgaggg
      421 ggacctggtg gtcgccgccg agtactgcac gccggacatc gtgaacttca tggtgaccca
      481 cggacgcggc ctcctctgcg tggcgatgga gcaggagcgg ctgaatgcgc tgggcctgcg
      541 gctcatggtg ccggagaact ccgacaagtt ctgcaccgcc ttcacggtct ccgtggatgc
      601 gcgccacggc accaccaccg gcatctccgc cagcgatcgc tcgctcacgg tgcgcgcgct
      661 catcgacgag cgcacggcgc cggacgacct cacgcgcccc ggccacgtgt ttcccctggc
      721 ggccaagcgg ggcggggtgc tcgtccgcgc gggccacacc gaaagctccg tggaccttgc
      781 gcgccttgcg ggactcaggc ccgccgcggt catttgcgaa atattaaaag aagacggcac
      841 catggcgcgc cggcccgacc tcgacgtctt cgccgaacgg cacggcctca agatcgccac
      901 catatcggac ctcatcaagt atcgccagca caaggagcgc ctcattcgcc gcgtctcctc
      961 cgcgaccctc ccgaccgact acggcaagtt ccacatcatg gcctacgaga ccgaggtgga
     1021 cgatgcgatg cacgtggccc tggtgaaggg cgacgtggat gggcgcgaga acgtgctggt
     1081 gcgcgtgcac tccgagtgtc tcaccggcga cgtgttcgcc tcccgccgct gcgactgcgg
     1141 ctcccagctc caccgcgcga tggagatgat cgacaacgag ggggcgggcg tggtgctcta
     1201 catgcggcag gaggggcgcg gcatcgggct gggaaacaag ctcaaggcct atcacctcca
     1261 ggaccagggc gtcgataccg tcgaggcgaa catcatgctg ggctttccgc ccgacctgcg
     1321 cgattacggc atcggcgcgc agatcctggt ggacctgggg ctccacacca tcaggctcat
     1381 caccaataac ccgaaaaagg tgattggcct cgaggggtac ggcctggaga tcgtggagcg
     1441 cgttccaatc gagatcgcgc cggtggacga gaacctgcgc tacctgaaga cgaaaaagga
     1501 aaagatgggc cacctcatcc tcgatgagaa gggcacgggg ggcccgcggt gaacgcgccg
     1561 gagacgccca ccgtcgtcgt cgtcgacgac gacgaggcca tacggtatgc gctgcagaaa
     1621 aagctcacgc gcttcggcct caagattgtc tcgctcgaca aggctgagga cgcgctcttc
     1681 ctgctgaaga accccgagag cgcggtggac ctcgtggtga ccgacatcaa gctgcgcaag
     1741 atggacggga tcgagctcct gcgacacatc aacatcctgg agcggccggt cccggtgctc
     1801 atcatcaccg gccagggaaa catcgaggac gcgatccggg cgctgcgcta cggggcgcgc
     1861 gacttcatcc gcaagccctt cgatatcaac gaggtggcct ccatcgtgcg gcgcatcctt
     1921 cgcagcgagc aggagaagca gctcgccagc gccttcggga agtacatccg cagcgaacgc
     1981 cgctcctacg tgatcccggt ggaccccggc gtgatcgacg tgatgtccta cgagctcacg
     2041 cgcaacctca tgcccttcgg cctgtgcaac tacaccgcct ccgagaacgt cgcgctggcc
     2101 ctgcaggaag cgatctcgaa cgcgatgttc cacgggaacc tcgagatcga ttccgcggtg
     2161 cgcgcggagc gcggcatcaa ggggttcaac gaggagatcg aggcgcgccg caccgacgaa
     2221 cgctatcgcg accggaccgt ccgcatccag tacgacctca accccgagta cgtcgagtac
     2281 gtgatcgagg acgacggccc cggcttcaat tacaatgcgc tgcccgatcc gcgcgatcag
     2341 cagaatttct tcaaggacag cgggcgcggc attctcatca tccgcatcca catggacgag
     2401 atctcctgga gcgagcgggg gaacgccatc cggatgcgca agtaccgcat cgacgcgaaa
     2461 tcggcccagc actagggcgg gaaaaggagc aatggcatgt actataccgt gaaatggaac
     2521 ggcgacgccg tctcgatgat cgaccagcgc gagctgccgg ggcgggtcgt gtaccgcacc
     2581 tacacgaact atcacgatgt cgcggcctcg atcaccgaca tggtcacgcg cggcgcgccc
     2641 gccatcggga tcaccgcggc gatggggatc gcgctggccg cgaagaccct cgaggtcacg
     2701 gacgcggcgg acctgaagac gcaactcgcg gcggtctgcg agctcttcgc gaagacgcgc
     2761 cccacggccg tgaatctctt ctgggcgatc gaccggatga aggcgatcat cgccgcgaac
     2821 gactccgtcg acggcatccg cgcggcgctg gagaaggagg cggtgcgcat cctcgaggac
     2881 gacgtcgcga tgaacaagaa aatcggcgcg aacggcgcga agtacatcgc cgacggcgac
     2941 gtggtgatga cgcactgcaa cgccggggcg ctcgcgaccg ggggctacgg cacggcgctg
     3001 ggggtgatcc gcgccgcggt ggaggcgggc aagaagatcg aggtgttcgc ctgcgagacg
     3061 cggccgtacc tgcagggcgc gcgcctcacc gcgtgggagc tgcagcagga caacatcaag
     3121 gtgacgctca tcaccgacaa catggccggg cacttcatgc gcgccggcgt catcaagaag
     3181 atcgtcgtgg gggccgaccg gattgcggcc aacggcgatg cggcgaacaa gatcggcacc
     3241 tacacgcatt cggtggtcgc caaggcgcac ggcatcccgt tctacgtggc cgcgcccacc
     3301 tcgacgctcg acccggccac cgaaacgggc gatgcgatcg tcatcgagga gcggggcatc
     3361 gacgaggtcg cctatatcca gggcgtccag atcgcgccgg aggggatctt catccgcaac
     3421 ccggccttcg acgtcacgcc cgccgagaac atcacggcca tcatcaccga gaagggaaac
     3481 atcgaaagcc caaagggcac gggcatccgg gggcttgtgg gcgcataggc gccccgggaa
     3541 tttcccttga cggcgcggcg ggtgcgtgat acgcacgggc gtcgccgtgc gcgggccgag
     3601 cagcgccgcg gcgggacgga catcgatcat acaacaacga ttcggaggat gcaatgaaac
     3661 gtagcagcgt agtgatcgca ctggtgtgcc tcgccgcgtt cgtggcgacc ggctgcaagc
     3721 cgagcgcgaa aaacgagatc cagtactggg aaaacgcgaa gaaggatttc gccgaggccg
     3781 tttcctcgta cccggccttc aaggacgtgc ttgccgcgaa gatgaccgag gcgcagaagc
     3841 tatgggacga tgcgcagaag gcgaccggcg aggagcaggc gaagcagatg aaggccgcca
     3901 acgagaagtt gaacgagctt ctcaaccagt tcacgcagat taagcacaag tccaagggcg
     3961 ttcaggacgc catcgactcg ctcaacgcga agaaactcac ggtgagcgag gacgcgaccc
     4021 ggaagaaggc cgtcgccgcc gcgcggaagg ccctgggcga ggtgaaggac ctgctcgcca
     4081 acgcgaaggt cgccgccgaa gacgaggcga agaagatcac cggcgaggcg atctcgaagc
     4141 tcatcagcgc gcagggcgac atcgaccgcg ccatcaagtc gctcgagccg aagaagtcga
     4201 gcaccgccaa gaagaaaaaa tagtcgcaga acgacgcagg tggccttgtc gggcgatcgg
     4261 cccgggcgtt ccgcccgggc cgtgtcgttt cctggctccg gggctcaaaa cggaattgac
     4321 ggcgtgatct tcatatggca tggcgtggtc gatgtgtgac gcgccatccg atcgcatgag
     4381 gttagaccat gaaaaaaacg atttattccc ttctgctgct ggcggttgtg ttgatttcat
     4441 cggttcccgc ccgttccggg caggagcggc ttcgcgttgc ggtgaacagc ttcgacaacc
     4501 ttaacaatga caagaagtat gcgcacctct ccgcgggctt cagcgaggcc ctgacccagg
     4561 ggctctttca gatcaagaac atactcgtcg tcgagcggca gggcctcgac cgcatcatgc
     4621 gggagcacga acttgcgcaa gcgggcgcga ttgacgaaaa aagcgccgtg aagacgggcc
     4681 ggcttatggg cgccaactac ctggtgatgg ggaaatatca gattgcgggc gggaatgtgc
     4741 gcgtgttctg ccggctggtg aatgtcgaga cgggcgcggt ctcacgggac cattcctttc
     4801 aggtgaccgg gagccttgat ggcattttca cggtgcagga tgagctcgtg aaacggatca
     4861 ccgcctcctt cgggatcagc cccggcacgg acgagcgtag gcgcgtggaa acggtcaacc
     4921 gcgaactctc gttgacgaaa gatgaattca agctctatct gcaggcgcgg aaggaattct
     4981 atctcgcgac ccccgactcc taccagcgcg ccattattct ctacgccaag gcgttgggga
     5041 tcaacgggaa attcgcgctc gcctgggcgg gactggcgac cgcaaacctg tacaaggggt
     5101 atgagcgaag ctggaacggc caatcatacg atgaggaata tttcaaggcg cttgcggcgg
     5161 cccggcaggc gatacgattc gcgccggacc tttcggagac caattgcgcc atggcgctcg
     5221 tacatgcata ctggcttccc cggccgaaac ggaaggaggc cgaggagtgg gcgcgcaagg
     5281 cgctggccat caacgaaaac aacgccgagg cgtatttcgc catatcaaag gcgcgcgggg
     5341 gcgatcttga gtatctcaag aagaccatcg agaaggaccc ccagtacatc ctcgcataca
     5401 actggcttgg tctggtttat caaaaaatga acatgatcga taaggccatc gagatgcatc
     5461 gcacgtccat acggcttaat ccgaaatggg cgctcgaata cgcgaatctc gcctattgtc
     5521 tgtttctgca aaagaaatac ccggaggcca tggagaatat caacagggcg ctgcagctca
     5581 agcccgatct caagccggcg cacgtgatca agggcatgat cgtcaaggcc tccggcggcc
     5641 gataggccca tcagaacatg attcggtagt agaggagcac gatgtcccgc ccgaagagga
     5701 gcgcgacgac caggcccgcc gcgaggaacg gcgcgaaggg gaagcgcacg cgcaggccct
     5761 tcttcgtccg gtacgcgtag atgacgcccg cgacggagcc ggtgaccagc gccgtttcga
     5821 tcatgacgat ggtgagctcg aacccgagga gaaggcccat cgccgccgcg agtttgatgt
     5881 cgccgccgcc gaagctcccg gggaacacga gcagcatcac cacgaagaag gccgcggcga
     5941 tgccgaatcc caggagcgtg tcccggaccg atcccgtgag gatgatgggg tagatcgaca
     6001 gcgcgacgaa cgcgagggtg agcgggagcg gtatttccat cgttttcgcg tcgatgatgg
     6061 cgatggcgat cgccagcccc atgaccagga agaggaccgc gccgggcgcg ttcacgccga
     6121 gccgcagggc gacgacgacg gcgagcgcgc catacccgag ctccgcgagc ggataggccg
     6181 cggggatccg cacgccgcag gagttgcagc gcccgcgcgc gatgaggtag ccgagcgccg
     6241 gaatgaggcg gagaagcggt atgcgcgatg cgcacgcggg gcagcgcgac ggcgtcgcaa
     6301 gcgcgcgcca ggggctctcg cgaaacgagc cgtccgccca gcgcagggcg agggtgtaga
     6361 agaagctccc ccatgcggcc ccgaggaaaa agacgacggc ccagagaatc gcaccgtgcg
     6421 ccatgggcta tatctgcagg tagtagttca cgccgatgaa ggcgaaatcg cccgtggcgc
     6481 tgaaggttcg cttcggcgac gtggttgccg cgccgttcgc gtccgtcccc ttgttctcga
     6541 gcgcctgcga gcgcgtggcg aggtcgtcgt gttcccgcgt tcccggt
//
LOCUS       DAORHF010000206         6503 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11808_length_6503_cov_5.953784, whole genome shotgun sequence.
ACCESSION   DAORHF010000206 DAORHF010000000
VERSION     DAORHF010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6503)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6503
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11808_length_6503_cov_5.953784"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /gene="cobA"
                     /locus_tag="PKJ16_14405"
     CDS             complement(<1..747)
                     /gene="cobA"
                     /locus_tag="PKJ16_14405"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HNV48233.1"
                     /translation="MVYLVGAGPGDPGLISEHARRALKNADCVVYDFLANAGFVDGLS
                     CELIYVGKEGSAHTMKQEDINRLIVEKARAGKTVVRLKGGDPFVFGRGGEEAEELIAA
                     GVPFRIIPGISSFYSAPAYAGIPLTHRDYADAFEVVTGHRRADAAAGEDINFPDFDDK
                     KTFVFLMGMKNLGYIAETLIAEKRFPADLPAAVIMWGATARQRVAAAALADIASEAAR
                     QGLAAPAIIVIGRVVALREKLRWFDSLPLFG"
     gene            complement(756..1664)
                     /gene="hemC"
                     /locus_tag="PKJ16_14410"
     CDS             complement(756..1664)
                     /gene="hemC"
                     /locus_tag="PKJ16_14410"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HNV48234.1"
                     /translation="MPAKIKIGTRGSELALWQANHVAERIGRDRVEIVIIKTQGDRIQ
                     NVSFDKMEGKGFFTKEIEEALLVRTVDLAVHSMKDLPVEETPGLVVAAVLEREDPSDV
                     LLVRDDRRDAARPFGLPAGAVVGTSSLRRAAQIRAIAPDIDVRPLRGNVGTRIRRLAE
                     KRFDAIMLARAGLLRLAPAIDGMTMEPIPLDRFLPAPAQGALALQVRADDAELIGVIR
                     ALDHADTRRSVTAERSFLMHFGGGCQIPLGAYARLENGAIILDGVVASRDGITIVRGN
                     VSGSDPERVGADCAHQLKKQGADSLL"
     gene            1843..2724
                     /locus_tag="PKJ16_14415"
     CDS             1843..2724
                     /locus_tag="PKJ16_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV48235.1"
                     /translation="MKQANDILFSICTWSLVLSLLLGGFVIAPSIIMPHRVPGIYSSL
                     FWIGIASIIVFVVSVMLILKQSQRANRASAITELRAAVREWKNGQFGEGEVLSLSHRL
                     EKQGSAEREILIKEIPTPMLHEVLRRDILPDPEILALAIAETAARHDRAALPLLRDIY
                     RDNSPEMIPHPVRRAIMRAFETTGNSRYASFLVGQLNEPDGDVLVGVIRALATSGTVK
                     TVEPLFRLGRDSMNPFVRTAAKDAIDAIQARLGTVDAGWLSLPVEPGLDGALSAPATD
                     RTGSLSHIATKMKKKKK"
     gene            2729..3025
                     /locus_tag="PKJ16_14420"
     CDS             2729..3025
                     /locus_tag="PKJ16_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48236.1"
                     /translation="MSDDKTINKTIGRIVTAAIGVVFMGFFITLLSRGESAGFVLKSL
                     FRIPFFHVISAEVFPNCEALMNGAWRRSWKVLVHRGMVISGVPTVHPACRFRNS"
     gene            3048..4307
                     /locus_tag="PKJ16_14425"
     CDS             3048..4307
                     /locus_tag="PKJ16_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV48237.1"
                     /translation="MNRTILLFTTLCIGIIFGAFAITLNSRGMPVGLVAKSLARLPVF
                     LIYPASEFENCAALAKADARALWKVRIGRATRVTDPFDFTCYAVDADSERPMLPDRIT
                     REIARDGGGSPIHRFNLAGSHGRILLTECGKHPFAINTTISGIAKPNTGDDTGVAMII
                     DNSHETPFLYPTVIFFWTFVLLGSITLARHQRTHNRPKVLHLELMAQQIVAEWEHRTP
                     VKNELDAMVALLGALDTMGQRRIMIQLPAAAFPAIIAADTATHDVLMEAVSLLVSRLR
                     KKSLPHLRDLFRRLDRQDAMKDVRLKALEMLQQLADRRQAPFLAGLLDHADDDILIGA
                     IRALAACGTVKEVERLYRLGQVRGDSTIRTAVREAIDAIQERLGKVDAGWLSLPDDST
                     LDGALSRPEIDRAGGLSDPEKDREKKK"
     gene            4390..4695
                     /locus_tag="PKJ16_14430"
     CDS             4390..4695
                     /locus_tag="PKJ16_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48238.1"
                     /translation="MEFIRIWTEKDIKEIQDHMLIVDDKFGFCPGCKQMGIALADLKQ
                     CPQCGREFKYMTSREKGNRGFNIVKWALKKLPHLTFVDAEDYDNLTGKKKAADLFNV"
     gene            4703..5650
                     /locus_tag="PKJ16_14435"
     CDS             4703..5650
                     /locus_tag="PKJ16_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV48239.1"
                     /translation="MEILVTGASGNLGSLLARHLLARGHTVRLMRHRTPLPDDIATHP
                     NARVHEADLARPETLAGPCRGVDAIVHFAGVLFMPRPERFLPTTNTEWFRNLMTAAIS
                     GNAKRVVLASFPHVEGETTPQRPATGRLDGSPESWHARTRLEEEKLLFSMTKGTGTAP
                     VSCRVGMVYGRGILMIDAARWLARHRLLAVWRKPTWIHLIATPDFLRALEAAATKNGA
                     SGIYHIGDRGVITLQEFLDRASARWGGRRPWRLPAWMIFTAAAMVELFAMAFGTQSPL
                     TRDFVRIGMASYYGDTTRMFDELIDTLEYPTFDTGIETL"
     gene            complement(5691..>6503)
                     /locus_tag="PKJ16_14440"
     CDS             complement(5691..>6503)
                     /locus_tag="PKJ16_14440"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV48240.1"
                     /translation="DIELFQATLRKALEYRRLKDLERDQLQRFRSELTRVREIQAASM
                     SRSRTPDGFDIAHSMLPAEDLSGDFYDGIRLRDDRTLVMLCDVTGHGIASSYVGAELK
                     SLFRSTAESTASPAEVLRDVNRTITERGRSSHYMNTAILCDIGSGGSSVVIASAGHPP
                     GIFYCAASGECQWVKGGGPLVGFTNQASYAERPIEMATGDCLLLYTDGLIEAFDEGGC
                     GMYGDERLMRVFADHVHAASRDIVLAIIDDVYRFTNYSRQHDDITIVCVKKI"
BASE COUNT         1240 a         2143 c         1968 g         1152 t
ORIGIN      
        1 cccgaagagc ggcagggaat cgaaccagcg cagtttttcg cgtaatgcca ccacccggcc
       61 gatcacgatg atggccggcg ccgcgagccc ctgccgcgcg gcctctgatg cgatatcggc
      121 caacgccgcc gccgcgacgc gctgtcgagc ggtcgcgccc cacatgatca ccgcggcggg
      181 caggtcggcc gggaatcgtt tctcggcgat aagcgtttcc gcgatgtatc ccaggttctt
      241 catgcccatg agaaagacaa aggtcttctt gtcgtcgaaa tcgggaaagt tgatgtcctc
      301 gcccgcggcg gcgtcggcgc ggcggtgtcc ggtgaccacc tcgaaggcgt cggcatagtc
      361 gcggtgcgtg agcgggatcc ccgcgtaggc cggcgccgag taaaacgacg agatgccggg
      421 gataatgcgg aatggcactc ccgcggcgat gagctcctcc gcctcttcgc cgccgcgccc
      481 aaagacgaac ggatcgcccc ccttcaagcg taccaccgtt ttgcccgcgc gcgccttctc
      541 cacaatgagc cggttgatgt cctcctgctt catcgtgtgc gcgctcccct ccttgcccac
      601 gtagatgagc tcgcaggaga gaccgtcgac gaaaccggca ttggcgagaa agtcgtacac
      661 cacgcaatcg gcgttcttaa gcgcgcggcg cgcgtgttcg gagatgaggc cgggatcgcc
      721 gggacccgcg cccacgagat aaaccatgcc gggtttcata gcagcgaatc ggccccctgt
      781 tttttcaatt gatgggcgca gtccgctccc acccgctcgg ggtcggagcc cgatacgttc
      841 ccgcgcacaa tggttatacc gtcgcgcgat gcgacaacgc catcaagtat gattgcgcca
      901 ttctcgaggc gggcatacgc tccgagcggt atctggcaac cgccgccaaa atgcatcagg
      961 aacgagcgct ctgccgtgac cgatcggcgc gtgtcggcat gatcgagcgc gcggatcaca
     1021 ccgatcagtt ccgcgtcgtc cgcgcgcacc tgcagcgcga gcgcgccctg cgccggggcg
     1081 ggaaggaacc ggtcgagcgg aatgggctcc atcgtcatgc cgtcgatggc gggcgcgagt
     1141 cgcaggagcc cggcgcgcgc cagcatgatc gcgtcgaagc gcttctccgc gaggcgccgg
     1201 atgcgcgtac ccacgttccc ccgcagcggc cgcacgtcga tgtccggcgc tatcgccctg
     1261 atctgcgcgg cgcgcctgag cgagctcgtg cccaccacag cgcccgcagg aaggccgaag
     1321 ggacgcgcgg catctcgccg gtcatcgcga accaggagaa cgtcggacgg atcctcgcgc
     1381 tcgagcaccg ccgcgacgac gaggcccggg gtttcctcga ccgggaggtc cttcattgaa
     1441 tgcaccgcga ggtccaccgt acgcacgagg agggcctcct cgatctcctt ggtgaagaag
     1501 cccttgccct ccattttatc gaaggagacg ttctggatgc ggtcgccctg cgtcttgatg
     1561 atcacgatct cgacgcggtc acgcccgatg cgctcggcca catgattggc ctgccacagc
     1621 gcgagctcgc tcccccgcgt tccgattttg atcttcgccg gcatggcatg cattctggtg
     1681 aaccgcaaag tccattgtca agggattata ccagagacag aataaattat ttacaacatg
     1741 cgacaggcat gtcattttct gcccattgat gtgcgaaaaa ttccgggctt tggttcccat
     1801 agtcggaatt gcgattttcg tgtggccgaa tacagaaata acatgaaaca agcaaacgat
     1861 atcctgttct ccatctgcac ctggagcctg gtgctcagcc tgctcctggg tggcttcgtc
     1921 atcgcgccat caatcatcat gccccatcgc gtcccgggaa tctattcgag cctgttctgg
     1981 atcggcatcg cctccattat cgtgttcgtc gtctccgtga tgctgatttt gaaacagtca
     2041 cagcgggcca atcgcgcttc cgccatcacc gaactccgtg cggccgttcg cgagtggaag
     2101 aacgggcaat tcggcgaagg cgaggtcctt tccctttcac accggctcga gaaacagggg
     2161 tccgccgagc gtgaaatcct catcaaggag atcccaacac cgatgctgca cgaggtgctt
     2221 cgtcgggaca tcctgccgga tccggagatc ctggcgctgg caattgccga gacggccgcc
     2281 cggcacgacc gcgcggcgct gccgctgctc agggatatat accgcgacaa cagtcccgag
     2341 atgatccccc atcccgtgcg gcgcgccatc atgcgcgcct tcgagacgac aggaaacagc
     2401 cgttatgcgt cattcctcgt cggccagctg aacgagcccg acggcgacgt cctcgtcggc
     2461 gtcatcaggg cgctcgcgac ctccggcacg gtgaagacgg tcgaaccgct gttccggctt
     2521 ggccgcgaca gcatgaatcc cttcgtgcgt acggcggcga aggacgccat cgacgcgata
     2581 caggcgcggc tcggcacggt cgacgccggc tggctctcgc ttcccgtaga gcccggactc
     2641 gacggcgcgc tcagcgcgcc ggcaactgac cgtacgggaa gtctcagtca catcgccaca
     2701 aaaatgaaaa agaagaagaa atgacgccat gagcgacgac aaaaccatca ataaaaccat
     2761 cgggcgcatc gtcacggccg caatcggcgt ggttttcatg ggcttcttca tcacgctgct
     2821 gtcgcggggc gaatcggccg gctttgtcct gaaaagcctg ttccgcatac cattcttcca
     2881 tgttatcagc gcggaggtgt tcccgaactg cgaagcgctc atgaacggcg cctggcgacg
     2941 cagctggaag gtcctcgtcc atcggggaat ggtgatatcc ggcgtcccga cagtacatcc
     3001 tgcctgccga ttccgcaaca gttagcgagc aaccggagag aatcgcaatg aacagaacca
     3061 ttcttttatt cacgaccctg tgcatcggta tcatcttcgg ggcatttgcc attacgctaa
     3121 actcgcgggg catgccggtt ggtctcgtcg cgaaaagcct tgcccgactt cccgtttttc
     3181 tcatttaccc cgccagcgag ttcgagaact gtgctgcact ggcaaaggcc gatgcgcgtg
     3241 cgctctggaa ggtgcgcatc ggacgggcaa cccgcgtgac ggatccgttc gacttcacct
     3301 gctatgccgt tgacgccgac agcgagcgtc ccatgcttcc cgaccgcatc acccgtgaga
     3361 tcgcacgcga cgggggcgga tcgcccatcc accgcttcaa tctcgccgga tcccacggca
     3421 ggatactcct gaccgaatgc gggaaacatc cctttgcgat caatactacg atcagcggca
     3481 tcgcgaaacc aaataccggc gacgataccg gcgtggcgat gatcatcgac aactcccacg
     3541 agacgccgtt tctgtatccc accgttatct tcttctggac cttcgtcctg ctgggcagca
     3601 taacgctcgc ccgtcatcag cgcacgcaca atcgtccgaa ggttttacac ctcgaactga
     3661 tggcgcaaca gattgttgcg gaatgggagc accgaacgcc ggtaaaaaac gagctcgacg
     3721 ccatggtcgc gctcctgggg gcgctcgata cgatgggaca gcgacgcatc atgatacagc
     3781 tgcccgccgc agcgtttccc gccatcattg ccgcggacac cgcaacgcat gacgtgctca
     3841 tggaagcggt ttctctcctc gtttcgcgcc tacggaaaaa atccctgccc caccttcgag
     3901 atctcttccg tcggctggac aggcaggacg cgatgaagga cgtgcgcctc aaggcgcttg
     3961 agatgctgca gcagctcgcc gaccgccggc aggccccgtt tctcgccgga ttgctcgatc
     4021 acgccgacga cgacatcctc atcggcgcga tccgcgcgct cgcggcctgc gggaccgtca
     4081 aggaggtcga gcggctgtat cgtcttggcc aggtgcgcgg cgattccacc atccgcacgg
     4141 cagtccggga ggcgatcgac gcgatccagg agcgcctggg gaaggtcgat gccggctggc
     4201 tctcgcttcc cgatgacagc acactcgacg gcgcactgag cagaccggag atcgaccgcg
     4261 ccgggggatt gagcgacccg gagaaggacc gggagaagaa aaaataaaag aatttacagg
     4321 gaatgcctcg aatgcatata ttcggcaccg ctcactgacg atagcgacac gggatatagg
     4381 aggcgaacca tggaattcat ccgaatctgg accgagaagg acatcaagga aatccaggat
     4441 cacatgctca tcgtggacga caagttcggc ttctgccccg gctgcaagca gatgggtatc
     4501 gcgctggccg acctgaaaca atgcccgcag tgcggccgcg agttcaaata catgacctcg
     4561 cgcgagaagg ggaaccgggg gttcaacatc gtcaagtggg cgctcaagaa gctcccgcac
     4621 ctcaccttcg tggacgccga ggactacgac aacctgacgg gcaagaagaa ggccgccgat
     4681 ctctttaacg tctgatccag ccatggagat cctcgtcacc ggcgcttcgg gcaacctggg
     4741 atcgcttctc gcgcggcacc tcctcgcgcg cggacacacc gtccgtctga tgcggcatcg
     4801 cacgcctctt cccgacgata tcgcgacgca cccgaacgcg cgcgtgcacg aggccgatct
     4861 tgcgcgcccc gaaacgctcg ccgggccctg tcgcggcgtc gacgcgatcg tccacttcgc
     4921 cggggtgctc ttcatgccgc gcccggagcg cttcctcccc accaccaaca ccgaatggtt
     4981 ccggaacctc atgaccgccg caatttcagg caacgcgaaa agggtggtcc tcgcgagctt
     5041 tccgcacgtt gagggcgaga ccacgccgca gcgtcccgcg accggccgtc tcgacggctc
     5101 gccggaatct tggcacgccc ggacgcggct cgaggaggaa aagctgctct tctccatgac
     5161 gaagggcacg ggcaccgccc cggtgtcgtg ccgggtgggc atggtgtacg gccgcggcat
     5221 tctgatgatc gacgccgcgc gctggctcgc ccggcaccgc ctcctcgcgg tgtggcgaaa
     5281 acccacgtgg atacacctca tcgcaacgcc cgatttcctg cgcgcgctcg aggcggccgc
     5341 gacgaaaaac ggcgcgagcg gcatctatca catcggcgac cgcggcgtca tcaccctgca
     5401 ggaattcctg gaccgggcga gcgcgcgctg gggcggccgg agaccatggc ggcttccggc
     5461 atggatgatc ttcacggccg cggcgatggt ggagctcttc gcaatggcgt tcggcacgca
     5521 atcgccgctc acgcgcgact tcgtccgcat cggcatggcg tcctactacg gcgacacgac
     5581 gcgcatgttc gatgagctca tcgacacgct tgaatatccg acctttgata cagggatcga
     5641 aacgctgtga cggcggtgaa tacgcacgga aaccgggcgg gccgcgcggc ctagatcttc
     5701 ttcacgcaca cgatggtgat atcgtcgtgc tgccgggaat agttggtaaa gcggtacacg
     5761 tcgtcgatga tcgccaggac gatgtctcgg cttgcggcat gcacgtgatc ggcgaatacc
     5821 cgcatgaggc gctcgtcgcc gtacatcccg cacccgccct cgtcgaaggc ctcgatgagc
     5881 ccgtcggtgt acaggagcag gcaatcgccc gtcgccattt cgatggggcg ctcggcatag
     5941 gacgcttgat tcgtaaaccc caccagcggt ccgccgccct tgacccactg gcactcgccg
     6001 ctggcggcgc agtagaagat acccggtggg tgcccggcgg acgcgatcac gacgctcgag
     6061 ccccccgacc cgatatcgca gagtatcgcc gtgttcatgt agtgggagct ccgtccccgc
     6121 tcggtgatgg tccggttgac gtcccgcagc acctccgccg gcgacgccgt cgattctgcc
     6181 gttgagcgaa acaggctctt gagctcggcc ccgacgtacg acgaggcgat cccgtgcccg
     6241 gtcacgtcgc agagcatcac gagcgtccgg tcgtcgcgca gacggattcc gtcgtagaag
     6301 tcgcccgaga ggtcctccgc cgggagcatg gaatgggcga tgtcgaaccc gtcgggcgtc
     6361 cgcgagcgcg acatcgacgc cgcctggatc tcgcgcaccc gggtgagctc gctgcggaag
     6421 cgctgcagct ggtcgcgctc gaggtccttc aggcgccgat actcgagggc cttccggagc
     6481 gttgcctgaa acagctcgat gtc
//
LOCUS       DAORHF010000207         6462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_11912_length_6462_cov_4.164195, whole genome shotgun sequence.
ACCESSION   DAORHF010000207 DAORHF010000000
VERSION     DAORHF010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11912_length_6462_cov_4.164195"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..765)
                     /locus_tag="PKJ16_14445"
     CDS             complement(115..765)
                     /locus_tag="PKJ16_14445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48241.1"
                     /translation="MRSSGKPEQPSPVCIREDVAGSIGGRSLTAIRRSCYAPSRASLY
                     DSSMQYRALIDLKDKSVLLIDDRNRSYVQITVDAFRSQAAEGMRLLAGPGGKRLPVRE
                     TGRTEKVQGRSCFEVSLFFEPQSLKMDLWLSKEIPAPLDAYFIFTANSGIGETFPGML
                     TLLRKHNAYIVESTMTMLRPGGLEVRLYSRLSGIEEIDQSDGAFAPPKGYEKLSVK"
     gene            complement(825..2279)
                     /locus_tag="PKJ16_14450"
     CDS             complement(825..2279)
                     /locus_tag="PKJ16_14450"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV48242.1"
                     /translation="MRKSLFRRICLILLTITSASCQNLQEELEKHLEMVRPTIVSVSP
                     ENNAQNVGSDTTVVITFSSEMDTVKTNNSFTLSSSAGVVKGYFAWSDGNTKMTFTPQN
                     ALVDSGQYMISLTTEAEDTTGNDLAGSHTSVFFVNYDINPPRVTGHTPLNGALGVPAT
                     PPGAPLPAGAAIQIDFSEPIDIDTIYDGISISPSVDGSWGWSAGQTSVYFYPMYDLSY
                     GTTYTVSINQSLTDAGGTPLDTPYFFNFTVGNDFEDPQIASVTQGAVTFTAGAVTPGV
                     EKQATIVITFNENINETTLYDAISLSPSCNHYITYDPAAFQATIHFVEAMESETNYLL
                     SIAGTIRDLQGNSLGSAANYYFLTNGPNSIRPTVTGIADPIVGAWSGVPVDLLMPIGT
                     VYPDNPQGIRVTFSTNMNPATITTKIEKVYGTGLMAYIINPSWPTAFSVFEFYIENIA
                     PGNTYTVTIKGGSVGARDANGNYMLNDHVTYIRF"
     gene            complement(2294..3883)
                     /locus_tag="PKJ16_14455"
     CDS             complement(2294..3883)
                     /locus_tag="PKJ16_14455"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HNV48243.1"
                     /translation="MKHTRNLIVTALLTIALFGCNNTKMIEDAVGALGDSFDMQTLAV
                     ISCSPSNHEYGLPVDTVVTVEFSSDVDRASVEQNFVLTTGSTPVPGSFDWKSGNSFQY
                     TPIDDLIVGSRYLIEIARETEDSRGNKMGAEFLSDFYVGGELEKPRVLSSTPAYTAGG
                     TTIDPATFTPREIVINFSEPMNRSSVEAAFSINPQLTGYFSWPTDSQMVYSITSDLEY
                     GRQYTARVTTAATDLAGNTLAGTYTLILLTGSVVTNPPPQVASITVDGVAMSTNPPPP
                     GYNTNVSRGTLSPLSPPQIVVTFTTTTGMNKAATESAFSITPSANGTFTWNAASDVLT
                     FIPSKQLASETIYTVRLNTGAEDVNGLNIPSDFQVRFMTNAADSLYVRVGDVLGTNDW
                     LAIPGFLFSETPAGWPTTIVMGPANPDSRDYFFTMRFVHGTPAVGPVVMDPTSVFTNV
                     SVIGFHPSGAFEPRIQDIRWNPAQTEVTIEVENLSNILLDAAVTGVPVLYRLTFTGGA
                     SGIKDANGNTMLENFSFDFRE"
     gene            complement(3885..5384)
                     /locus_tag="PKJ16_14460"
     CDS             complement(3885..5384)
                     /locus_tag="PKJ16_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HNV48244.1"
                     /translation="MKLFKKSKDTIRSVSGMLNDRVPKPAILAGKRRYVVAGVLALLV
                     IIAVSSIFQYYRWRTLRKTDDTGEIQTENVGIQMFTVERKDMIDELTILGQIVFLEKI
                     NISSKVTGRLERITVAEGTRVSKGQFLAGIEKLPLQLTLTQQQAELEIAKRSYDLSKA
                     KYENAIKAIEIKLKTIEKAEADLEDKKVSYKNTDRMLKNKIELFKAGGVSESDLASLK
                     ANHKTMYTKYLLAKSDLDIQKVGFRDQDLISEGLPVPKNAKEKYELFQRINTKIERAE
                     LEAAKSKITQVEKSIESTRILLSETDIRSPITGIVAAKSMEAGEMVKQESVLLTLVNI
                     SRVFVAMNINEKDIRRIKSDQEVHFTVDALGDEKFKGRISRINPVLDVKTRTVEVKAA
                     LENPGMRLLPGMFARANINCGNLKDIIAIPVSALLRKDTTTGELYVVKQGIVFKQAVE
                     LGRELKSDIEITKGLSDGDVIVIKGLNLLYPGAKIRTDEKKTASPAEVR"
     gene            complement(5362..>6462)
                     /locus_tag="PKJ16_14465"
     CDS             complement(5362..>6462)
                     /locus_tag="PKJ16_14465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV48245.1"
                     /translation="SRNDYRIALNLLIMEIRNSYFQVLQLRDSIEIHKKTLEHGLMQL
                     KFITKEYELGDATKFDKLEIETKVKEIEYNLEKACDEYIAALNQFKFLLKIDWRHPIE
                     VAGSIQDDFSIRPVSRKYDLDYMASLAMKNRKEVESADVDLLVKKKSYLMNELYYFPT
                     FAINLNYSLTDDKFMPRKKGWGIGFEVSAALFGSSGNVNSTYRKDDNNNSRSVTNSGS
                     ANILDNMAYKRNIVESKIELNRANDSAQQIRQQVALEISTAFSALNNSWEMIGISRKR
                     LELYDTQLVIERLKANMGESRRYDLMKKEIERGEAAIAHLASLVRYLVSASTLETSMG
                     ADIGFLQVSKYSKKYTGKIKNGGNGHEAIQKK"
BASE COUNT         1267 a         1661 c         1867 g         1667 t
ORIGIN      
        1 tgcgtatttc atcgagaaac gagcgttgga tagcattcgg tgatgcttca tccccctatt
       61 gtcttttata agggcgaacg gaattgctaa gctctccgcg taggacaaaa atccctattt
      121 cacactcagt ttctcatatc ccttcggggg tgcaaaagcc ccgtcggact ggtcgatctc
      181 ctcgatcccg gacagccggc tgtacaggcg cacctccagc ccgccggggc gcagcatggt
      241 catcgtgctt tccacgatgt acgcgttgtg ctttctcagg agcgtaagca ttcccgggaa
      301 agtctctcca atcccgctgt tcgcggtaaa aatgaagtac gcgtccagag gcgccggaat
      361 ttcctttgaa agccataggt ccattttcaa cgattgcggt tcaaaaaaaa gcgacacctc
      421 gaagcacgag cggccctgga ctttttcggt tctgccggtt tcgcgcaccg gcaatcgttt
      481 gccgcccggc ccggcgagaa ggcgcatccc ctccgcggcc tgcgagcgga atgcgtccac
      541 tgttatctgc acgtatgacc ggttgcgatc gtcaatcagg agcacgcttt tatctttgag
      601 gtcaataagc gcgcggtatt gcatcgagga gtcgtacagc gaggcgcgcg atggcgcata
      661 gcaggatcgt cgtatggcgg ttaagctcct gccaccgatg ctgccggcca catcctcgcg
      721 gatgcagacc ggggagggct gttccggctt tccgctcgat cgcatgtgcg aacctcccag
      781 aatgagaacg gcaaggaaga gtgacggcag cagtcgcctc atcactagaa ccgtatgtat
      841 gtgacgtggt cgttcagcat atagttgccg ttcgcgtccc gggcgccgac acttccgccc
      901 ttgatggtca ccgtgtaggt gttgccgggc gcgatgtttt cgatgtagaa ttcgaaaacg
      961 gagaatgccg tgggccagga cgggttgatg atgtaggcca taagtccggt cccgtacact
     1021 ttttcgattt ttgtcgttat ggtggccgga ttcatgtttg ttgagaaggt gacccgtatg
     1081 ccctgggggt tgtcgggata caccgtgccg ataggcataa gcaggtcaac agggaccccg
     1141 ctccaggcgc ccactattgg atcggcgatg cccgtaaccg tcgggcgaat ggagtttggc
     1201 ccattggtga gaaaatagta gttcgcggcg ctgccgaggc tgtttccctg gaggtcgcgg
     1261 atggtcccgg cgatgctaag gaggtagttg gtctcgctct ccatcgcctc cacgaagtgg
     1321 atggtcgcct ggaaggcggc ggggtcatag gtgatatagt ggttacacga cggcgaaagg
     1381 gaaatggcgt cgtacagtgt tgtctcgttg atgttctcgt tgaaggtgat gacgatagtg
     1441 gcctgcttct cgacgcccgg tgtaactgcg ccggcggtga aggtgacggc gccctgtgtt
     1501 accgaggcga tctgtggatc ttcgaagtcg ttgccaacgg taaaattaaa aaagtaggga
     1561 gtatccagtg gcgtgccgcc cgcatcagtg aggctctggt tgatcgatac cgtgtaggtg
     1621 gtcccgtagc tcaggtcgta catgggatag aagtacacag acgtttgtcc tgcgctccac
     1681 ccccagctcc cgtcgaccga tggtgaaata ctaattccat catagatggt gtcgatgtcg
     1741 atcggttcgg agaaatcgat ctgtatggcg gctcccgccg gcagcggagc gccgggaggc
     1801 gtcgccggga cacccagggc cccattgagt ggcgtgtgcc ctgtcacacg tggcggatta
     1861 atatcgtagt tcacgaaaaa aaccgacgtg tggctcccgg caaggtcatt ccccgttgtg
     1921 tcctcggcct ccgtggtgag gctgatcata tactgcccgc tgtcgacgag tgcgttctgg
     1981 ggcgtgaagg tcatcttggt gtttccgtcc gaccatgcaa aatagccctt caccactcca
     2041 gcggaggagc tgagcgtgaa actgttgttg gtcttaacag tgtccatctc gcttgaaaac
     2101 gtgatcacga ctgtcgtgtc gctgccaaca ttctgcgcat tgttttccgg gctcacagac
     2161 acgatcgtcg ggcggaccat ctcgaggtgc ttctctagct cctcttggag gttctggcat
     2221 gatgcggatg tgatcgtgag cagtataagg catatgcggc gaaagagtga tttcctcata
     2281 gtcgagatgc ctcctactcc ctgaagtcga agctgaagtt ttcgagcata gtgttcccgt
     2341 tggcgtcctt gatgccgctg gccccgccgg tgaaggtcag gcggtacagc accggcacac
     2401 cagtgacggc agcatcgagc agaatgttcg agaggttttc cacctcgatg gttacctcgg
     2461 tttgcgcggg attccatcgg atgtcctgga tgcgcggttc gaaggcgccc gacggatgga
     2521 atccaatcac gctcacattg gtgaagacgc tcgtcgggtc catcaccacc ggtccaacgg
     2581 cgggcgtgcc atgtacgaat cgcatggtga aaaagtaatc acgagagtca gggttggccg
     2641 gtcccatgac aatggttgtc ggccaaccag cgggtgtttc cgaaaacagg aatcccggta
     2701 ttgccagcca gtcatttgtg cccaggacgt cgcccacacg gacatataga gagtctgcgg
     2761 cgttggtcat gaaccgaacc tggaagtcag agggaatgtt gaggccattg acgtcttccg
     2821 cgccagtgtt gagtcggacg gtgtagatgg tttccgaggc gagttgcttg gatggaatga
     2881 aggtgagcac gtcagacgcg gcgttccagg tgaatgtgcc gttggcgctc ggcgtgatcg
     2941 aaaaggcgga ttcggtcgcc gccttgttca tgcccgtcgt cgtggtgaat gttacaacga
     3001 tctgtggcgg gctcagcggc gaaagcgttc ctcggctgac gtttgtattg tagcccggcg
     3061 gcggaggatt cgtgctcatt gcgacgccgt ccacggtgat tgacgcgact tggggagggg
     3121 gattggtcac tacgctacca gtgagcagga tgagggtata ggttccagca agcgtgttgc
     3181 ccgcgagatc ggttgccgcc gtggtaaccc gggcggtgta ctgccgcccg tattcaaggt
     3241 ccgaggtaat cgaatagacc atctggctgt cagtgggcca cgagaaatag cccgttagct
     3301 gggggttgat gctgaacgcc gcctccacgg aagaccggtt catcggttcg gaaaagttga
     3361 taacgatttc gcggggagtg aaggtggccg ggtcgattgt tgtgccgccc gccgtgtagg
     3421 cgggggtcga tgatagcacg cgcggctttt caagttcgcc gccgacgtag aaatcggaaa
     3481 gaaactccgc ccccatcttg ttgccgcgcg agtcttcggt ctcgcgtgcg atttcaataa
     3541 gataccggga acccacaata agatcatcaa tcggggtgta ctggaaggag ttgccggact
     3601 tccagtcaaa actacccggg acgggggtcg atccggttgt gagcacgaaa ttttgttcaa
     3661 cgctcgcccg gtccacatcg ctgctaaatt cgactgtgac caccgtatct acgggaagcc
     3721 cgtattcgtg gttcgatgga ctgcaagaaa taaccgcgag cgtctgcatg tcgaatgagt
     3781 ctccgagggc gcccactgcg tcctcgatca tttttgtatt gttgcagccg aaaagtgcga
     3841 tcgtcaggag tgccgtgaca atcagatttc ttgtgtgctt catcttaacg cacctccgcg
     3901 ggcgatgctg ttttcttttc gtccgttctg atctttgcac ccggatagag gagattgagg
     3961 cctttgatta cgatcacgtc gccgtctgac agacccttgg tgatttcgat gtcggatttc
     4021 aattcgcgtc ccagttcgac cgcctgcttg aagacgatcc cctgcttgac gacatacagt
     4081 tcgccggttg tggtatcttt tctcagtagg gccgataccg ggatggcgat gatgtctttt
     4141 agattaccac aattgatgtt agcgcgggcg aacatgccgg gcaaaaggcg catgccggga
     4201 ttctcgagcg cggccttcac ctctacggtc cgggtcttca cgtcgagcac agggttaatg
     4261 cgagatatcc gtcccttgaa tttttcatcg cccaaggcgt caacggtgaa atgaacctcc
     4321 tggtcggatt tgatccgcct gatgtccttc tcgttgatat tcattgcgac gaatactctc
     4381 gagatgttta ccagagtcaa tagcacggat tcctgtttca ccatctcgcc ggcttccatg
     4441 cttttcgcgg caacgattcc ggttattgga gaacggatat ccgtctcgct taaaaggatc
     4501 ctggtcgatt cgatgctctt ttcgacctga gtgatcttgg atttcgccgc ttcaagttca
     4561 gctcgctcga tttttgtgtt gattctctga aagagttcgt acttctcctt cgcgtttttt
     4621 ggaaccggca atccctcgct gatgaggtcc tggtcgcgga aacccacctt ctggatgtcg
     4681 aggtccgatt tcgcgagaag gtacttggtg tacatggtct tgtggtttgc cttcagcgat
     4741 gcgagatcgc tttcactgac gccgcccgcc ttgaaaagct cgattttgtt tttaagcatt
     4801 cggtccgtgt tcttgtagga gaccttctta tcctcaagat cggcttcggc tttctcaatc
     4861 gtcttgagct tgatctcgat tgccttgatg gcgttttcgt atttcgcctt ggacaaatcg
     4921 tatgaccttt tcgcaatctc aagctccgct tgctgctggg ttagggtgag ttgcagaggg
     4981 agtttttcga tgccggcaag gaactgtccc tttgatactc ttgtcccttc ggctacggtg
     5041 atccgttcca ggcggccggt taccttcgag gagatattga tcttttccaa gaaaacgatc
     5101 tggccgagga tggtcagctc gtcgatcatg tccttgcgtt caacggtgaa catctggatg
     5161 ccaacatttt cggtctggat ttcgccggtg tcgtctgttt ttcgcagggt gcgccagcgg
     5221 taatactgga atatcgaaga gacggcgatg atcaccagga gcgcaagcac gccggcaacg
     5281 acatacctcc gttttcctgc cagaatcgct ggtttcggca ccctgtcgtt gagcatgccc
     5341 gatacggatc gtatggtgtc tttacttttt ttgaatagct tcatgtccgt ttccaccgtt
     5401 cttgatcttg cctgtgtatt ttttgctgta ttttgaaacc tggagaaagc cgatatcggc
     5461 tcccatcgac gtttcaagcg ttgatgccga aacgagatag cgcacgaggg aggcgagatg
     5521 cgctattgct gcttcacccc gctcgatctc ctttttcata aggtcgtaac gacgggattc
     5581 ccccatattt gccttgaggc gctcgattac cagttgggtg tcatacagct ccagccgttt
     5641 tctggatatg ccaatcatct cccacgagtt gttaagggcg gaaaaggcgg tcgagatctc
     5701 gagggcaacc tgttggcgga tctgctgcgc ggagtcgttg gcacggttga gttcgatctt
     5761 gctctccaca atattacgct tgtaggccat gttgtccagg atgttcgcgg atccgctgtt
     5821 ggtgacgctt cggctgttgt tgttgtcgtc ttttcgatag gtggagttca cattgccaga
     5881 gcttccaaac agagccgctg acacctcgaa gccgatgccc catccttttt tcctcggcat
     5941 gaacttgtcg tcggtgaggg aatagtttag attgatggcg aaggtgggga agtaatagag
     6001 ctcgttcatg agataagact ttttcttcac taaaaggtct acgtcggcgc tttcgacttc
     6061 cttgcgattc ttcatcgcga gtgaggccat gtagtcgaga tcgtacttcc tgctcaccgg
     6121 tcggattgaa aaatcatcct ggatgctgcc ggccacctcg atgggatgac gccagtctat
     6181 cttgagtagg aatttaaatt ggttcagggc cgcgatatac tcatcgcatg ccttctcgag
     6241 attatactca atttccttca ccttcgtttc gatctcgagc ttgtcgaact ttgtggcatc
     6301 gcccaattcg tattccttgg ttatgaactt gagctgcatc aggccgtgtt ccagggtctt
     6361 cttgtggatc tcaatcgagt cccgaagctg tagcacctgg aaataactgt tcctgatctc
     6421 cattatgagg aggttgagtg cgatgcgata atcgtttctt ga
//
LOCUS       DAORHF010000208         6427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12019_length_6427_cov_5.761142, whole genome shotgun sequence.
ACCESSION   DAORHF010000208 DAORHF010000000
VERSION     DAORHF010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6427
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12019_length_6427_cov_5.761142"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="PKJ16_14470"
     CDS             <1..126
                     /locus_tag="PKJ16_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HNV48246.1"
                     /translation="VVQATGVGTRATAEDFIALIRDAGKTPVLRNSRYEIIRRYD"
     gene            158..883
                     /locus_tag="PKJ16_14475"
     CDS             158..883
                     /locus_tag="PKJ16_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HNV48247.1"
                     /translation="MPARGESTIPCPMDEYCSPTWFIESAHPAVVAFARDAAGDGDAT
                     ARAVRLYYAVRDGIAYNPYRYTFERGPYRASAVLAAREGFCVQKSILLAAAARAIGIP
                     CRVGYANVINHLSTKRMRELLRTDLFVFHGYNEFHLGGRWVKATPAFDAALCQRFGLR
                     PLEFTGAEDSIFHPYDLAGRRHMEYVHDYGTFADFPFETMVRESLRHYPHIEALMREH
                     DAGARIEDPELAGTPDPSPRSPE"
     gene            880..1590
                     /locus_tag="PKJ16_14480"
     CDS             880..1590
                     /locus_tag="PKJ16_14480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HNV48248.1"
                     /translation="MSLRYEEHHDHPAERKRFIRGLFDSIVPTYDLLNRLLSMGIDGG
                     WRRATVRALGDLRGRTTLDLCCGTGDLSRLLSRAGAAVVSLDFSLPMLSTGVAKGKII
                     GRPVAADASALPLADASIDAATIAFGIRNIPDIPRMLAELHRVMRPGGRFAILELTRP
                     RNRLIGAAYRFYLAHALPVIGGMISGRRTAYRYLAGTIDTFLDPGELCAAIERAGFRD
                     AVADGKTFGIATIITCRR"
     gene            1702..3861
                     /locus_tag="PKJ16_14485"
     CDS             1702..3861
                     /locus_tag="PKJ16_14485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48249.1"
                     /translation="MNFYILIPLAFFTINLCLVVYIAAKSGKKLAARAFMLFAVMLAL
                     WELSEFFLWSNLPDATLIGIYRATGILYMLPGVLFLNFAYSLLGKKRDAVFHGFSACM
                     FAGTILAFASDWYFPDRIIRHGWGNAAAPGPLMYPIIIVAIVLPGLYGIALIAAAMRT
                     QRDPLRVRQFGTVLAGFAAATAAAVLTNVVLPLFADAAAIPHLASSLAGIGSISMFIA
                     IARYRLLAPGLGDAAVEIFSRVHDCVFVVGSDGRVLEMNEAAARAFPQAGPDAGAPGL
                     AALYGELTCVHDECRDRETTLSLGGESRTYLVSQSPIGGSGESPATLVVMKDITEMRR
                     MEAELSRSSRIESLGVFAGGIAHDFNNLLTVILGNIALVRQSIQRDPEALRMLAQAET
                     ASIRAADLTRQLLTFSKGGDPVKRITSLPALVRETMQFALSGSSVRCAYDFAEDLLNA
                     EIDANQIGQVIHNIAINALQAMPRGGAITVGAHNCGDDGGGAIGPHVRLTFADTGCGI
                     PAGIIERIFDPFFTTREMGTGLGLSIAYSIVTKHGGRICVSSRPGDGTMFEVYLPATD
                     ARVVARCEDAIPAPGRPLKILVVDDEPQVRDIASRILSALGHRAACVGSIDDAVTAFS
                     AAHARGERFDCVITDLTIPGGAGGTEVFRLLREIDRNVTILISSGYSNDPILAEHARH
                     GFAGIIPKPYRIEDVRNALLRINDEPPAPDDGLSRVN"
     gene            3945..4160
                     /locus_tag="PKJ16_14490"
     CDS             3945..4160
                     /locus_tag="PKJ16_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48250.1"
                     /translation="MGTALVHLIGLILLLVGWFVTIINVGMTFTVGVTKFSASMFNSG
                     TLVGLVIIIIGAYVPMVGGIILNKLGK"
     gene            complement(4180..5235)
                     /locus_tag="PKJ16_14495"
     CDS             complement(4180..5235)
                     /locus_tag="PKJ16_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48251.1"
                     /translation="MRIGTGATVLCALMAVAIGVIGAGCSSAVKIERAETISVGRAVR
                     AYYDAARARRLTTTVWYPAKGVARGWEAPSVNMFHGNVNSREGLEPAAGTHPLILFSH
                     GARGAADNYSWFAEALAMKGFMVMGVDHHHDTWHSSTPEGTVSVWERPRDLSVALDMV
                     LADTVLGPLVDRARIGAAGHSSGGYAALALAGARYDILRMAEYCARNGKVPACRFGED
                     VKWWGLLKELDASRGSFRDDRVRAVFAMAPAMGDGIVPESLKEISVPVVIIAGEKDEF
                     VPFADNAKRYAEAIPKAELVPLAAGEGHYVFLDQCTWMGAGLIEELCADATDVDRAAA
                     HRTTASRAASFFSGALK"
     gene            5430..>6427
                     /locus_tag="PKJ16_14500"
     CDS             5430..>6427
                     /locus_tag="PKJ16_14500"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HNV48252.1"
                     /translation="MKKRLFVVDGHALCYRAYFAFIKNPLVTADGQNVSAIYGFALML
                     LKLINDQRPDYLAVAFDPPRKSFRFRMYPEYKATRQKMPDDLRSQIDEIKHMLSVLGI
                     VRVEEEDFEADDVLGTLATTHGSKSLEVVLVTGDKDAYQLVGPHVRIYAGKKGITEFD
                     IYDAKAVEEKLGVTPTQVIDYMALTGDASDNVPGVKGVGEKTALQLITDYKTLDGVYA
                     HVDEITGKLKDKLVESKEMAYLSRDLVTIRTDLPLQFDIEQSALPDLKTPTVRSYFAQ
                     YEMKSILKEIFGDGAGGDDNAAAAPGTDRATRDYRTVRTADELAAACAAIRAAGEVS"
BASE COUNT         1111 a         2222 c         1995 g         1099 t
ORIGIN      
        1 gtggtgcagg cgaccggcgt ggggacgcgc gccacggcgg aggacttcat cgcgctcatt
       61 cgcgatgcgg gaaagacgcc cgtgctgcgc aattcgcgct acgagatcat ccgccgatac
      121 gactgaccgg ccgaccctcc gcccgcacgg aaaatgcttg ccagcgcgcg gggaatcgac
      181 catcccatgt cccatggacg agtactgttc gccaacctgg ttcatcgaga gcgcgcaccc
      241 ggcggtcgtc gccttcgcgc gtgacgccgc cggcgacggc gacgcgacgg cccgcgccgt
      301 gcggctgtac tatgcggtgc gcgacggcat cgcctacaat ccctatcgct acaccttcga
      361 gcgtgggccg taccgcgcct cggcggtgct cgcggcccgc gagggatttt gcgtccagaa
      421 gtcgatcctc cttgcggcgg cggctcgcgc gatcggcatc ccctgccgcg tggggtacgc
      481 gaacgtgatc aaccacctct ccacgaaacg gatgcgcgag cttctgcgca cggacctgtt
      541 cgtctttcac ggatacaatg aattccattt gggcggcaga tgggtcaagg cgacgccggc
      601 cttcgacgcc gcgctgtgcc agcgcttcgg gctgcgtccg ctggagttta ccggcgcgga
      661 ggattcgatc ttccacccgt acgatctcgc gggacggcgc cacatggagt acgtgcacga
      721 ctacgggacc ttcgcggact tccccttcga gacgatggtg cgcgaatcgc tgcgtcatta
      781 tccgcacatc gaagcgctca tgcgggaaca cgacgcgggc gcgcgcatcg aggaccccga
      841 gctcgccggg acgcccgatc cgtcgccgcg gagcccggaa tgagcctgcg ctacgaagag
      901 caccacgacc accccgccga gcgcaagcgc ttcatccgcg gtctcttcga ttccatcgtc
      961 cccacctacg acctcttgaa ccgcctgctc tcgatgggga tcgacggcgg ttggcggcgc
     1021 gcgacggtgc gcgccctggg cgacctgcgc ggccggacca cgctcgatct gtgttgcggc
     1081 acgggcgatc tctcgcgtct gctctcgcgc gccggggccg ccgtcgtctc gctcgacttc
     1141 tcgcttccca tgctctcgac gggcgttgca aagggaaaaa tcatcgggcg tcccgtcgcc
     1201 gccgacgcat cggccctccc gctggccgac gcctccatcg acgccgcgac catcgccttc
     1261 ggtatccgca atatccccga catcccgcgc atgctggcgg agctccaccg cgtgatgcgc
     1321 cccggggggc ggttcgccat ccttgagctc acgcggccgc gcaatcgcct catcggcgcg
     1381 gcgtaccggt tctatctggc gcatgcgctt cccgtcatcg gggggatgat ttcgggccgc
     1441 aggaccgcct accgctacct cgcggggacc atcgacacct tcctggaccc gggcgagctg
     1501 tgcgcggcca tcgagcgcgc ggggtttcgc gatgcggtgg cggacgggaa aacgttcggg
     1561 atcgcgacca tcatcacctg ccgacgctga acgccgcatg ctgccatgcg ccatcggtcc
     1621 gaaggacggt tgcgacgccc gtatcctgct tgacaaaaac attcgatggg gcaatgatca
     1681 atacgcacac catggtggag aatgaacttc tatattctca tcccgctcgc gtttttcacg
     1741 atcaacctgt gcctggtcgt ctacatcgcc gcgaaaagcg gcaaaaaact ggcggcgcgc
     1801 gcattcatgc tcttcgccgt catgcttgcc ctctgggagc tctccgagtt ttttctctgg
     1861 tcgaaccttc ctgacgcgac gctcatcggc atctaccgcg caaccggcat tttatacatg
     1921 cttcccggcg tgctcttttt gaatttcgcg tactcgctcc tcggaaaaaa acgggacgcc
     1981 gttttccatg ggttttccgc ctgcatgttc gccgggacga tactggcgtt tgcgagcgat
     2041 tggtacttcc cggatcgcat tattcggcat ggatggggga acgccgccgc gcccgggccg
     2101 ctcatgtacc ccatcatcat cgtcgccatc gtcctgccgg gcctgtacgg gattgcgctc
     2161 atcgccgccg ccatgcgcac acagcgcgat ccccttcgcg tgcggcaatt tgggaccgtc
     2221 ctcgccggtt tcgccgcggc caccgccgcc gcggtgctca ccaatgtcgt gttgccgctg
     2281 ttcgccgacg cggcggcgat cccccatctc gcctcatcgc tggccgggat cggctcgatc
     2341 tcgatgttca tcgccatcgc ccgctaccgc ctgctcgcgc ccgggctcgg cgacgccgcc
     2401 gtggagatat tctcccgcgt gcacgactgc gttttcgtcg tcggaagcga cgggcgggtg
     2461 ctcgagatga acgaggcggc cgcgcgcgcc ttcccgcagg cggggcccga tgcgggagcg
     2521 cccggcctcg ccgcgcttta cggcgagctc acctgcgttc acgatgaatg ccgcgatcgt
     2581 gaaacgaccc tgtcgctcgg cggggagagc cgcacctacc tcgtctcgca gagccctatc
     2641 ggcggcagcg gggagtcccc ggccacgctc gtggtcatga aagacatcac cgagatgcga
     2701 cggatggagg cggagctatc gcggagctcc cggatcgagt cgctgggcgt tttcgcgggc
     2761 ggcatcgcgc acgatttcaa caatctcctc accgtgatcc tgggaaacat cgccctggtc
     2821 cggcagagca tccaacgcga ccccgaggcc ctgcgcatgc tcgcccaggc cgagaccgcc
     2881 tccatccggg ccgccgacct cacgcgccag ctcctcacct tttccaaggg gggcgatccg
     2941 gtgaagcgta taacgtcact ccctgcgctc gtcagggaga cgatgcagtt cgccctgtcg
     3001 ggatcgagcg tcaggtgcgc ctacgacttc gcggaggacc tgttgaacgc tgagatcgac
     3061 gcgaaccaga tcgggcaggt gatacacaat atcgccataa acgccctcca ggcgatgccg
     3121 cggggcggcg ccatcaccgt aggcgcgcac aattgcggcg atgacggcgg cggcgcgatc
     3181 gggccccatg tgcggctcac gttcgcggac accggctgcg ggatccccgc cggcatcatc
     3241 gagcggatat tcgacccatt cttcaccacc cgcgagatgg ggaccggcct ggggctgtcg
     3301 atcgcctact ccatcgtcac gaagcacggc gggcgcatat gcgtgtcctc gcgccccggc
     3361 gacggcacga tgttcgaggt atatctgccg gcgaccgatg cccgcgttgt agcgcgctgc
     3421 gaggacgcga tcccagcacc gggacgcccg ctcaagatac tcgtggtgga cgacgaaccg
     3481 caggtgcgcg atatcgcctc ccggatcctc tcggccctgg gccatcgcgc cgcatgcgtc
     3541 ggttcaatcg acgatgccgt gaccgcgttt tccgcggccc atgcgcgggg cgaacgcttc
     3601 gattgcgtga tcaccgacct caccatcccc ggcggcgcgg gcggcaccga ggtgtttcgc
     3661 ctcctccggg agatcgaccg gaacgtgacc attttgattt ccagcggata ttcaaacgac
     3721 cccatactcg ccgagcacgc gcgccacgga tttgccggga tcatccccaa gccctatcgc
     3781 atcgaggacg tgcggaacgc gctcctgcga atcaacgacg aaccgccggc gcccgatgat
     3841 gggctttcgc gcgtgaatta aaaaatgatt gcgctcccgc cgcacccggt gcttgagtgg
     3901 gatacgactc ggcaagacta catcttccac acggagtgtg acatatggga acggcgttgg
     3961 ttcatctgat cgggctgata ctgctgctgg tgggatggtt cgtgaccatc atcaacgtgg
     4021 gcatgacctt tacggtgggc gtgaccaagt ttagcgcctc gatgttcaac tcgggaacgc
     4081 tcgtgggcct ggtgatcatc atcatcggcg cctatgtgcc gatggtcggc ggcatcatcc
     4141 tcaacaagct gggaaagtaa cagcgggacc gaaccgcacc tacttcagcg caccggagaa
     4201 aaacgacgca gcccgggagg ccgtcgtgcg gtgggccgcg gcgcggtcga cgtccgtcgc
     4261 gtcggcacag agctcctcga tgagccccgc gcccatccac gtgcactgat cgagaaagac
     4321 gtaatggccc tccccggcgg cgagcggcac aagctcggcc ttggggatcg cctcggcgta
     4381 gcgcttcgca ttgtcggcaa agggaacgaa ttcgtccttt tcccctgcga tgatcacaac
     4441 cggcactgag atctccttca ggctttcggg aacgataccg tcgcccatcg cgggcgccat
     4501 ggcgaacacg gcgcgcacgc gatcatcgcg gaaggagccg cgcgatgcgt cgagctcctt
     4561 gagcagcccc caccacttca cgtcctcgcc gaaccggcat gcggggacct tcccgttgcg
     4621 cgcgcagtac tcggccatgc gcaggatgtc gtaccgggcc ccggcgagcg ccagcgccgc
     4681 atagccgccc gacgaatggc ccgccgcacc gatgcgcgcg cggtcgacga gcggacccag
     4741 gacggtatcc gcgagcacca tgtccagcgc cacggagagg tcgcgcgggc gctcccatac
     4801 ggacaccgtt ccctccggcg tgctcgaatg ccaggtgtca tggtgatggt ccacgcccat
     4861 gaccatgaat cccttcatgg ccagcgcctc ggcgaaccag gaatagttat cggcggcgcc
     4921 gcgcgctccg tgcgaaaaca gaatcagcgg atgcgttccg gccgcgggct caaggccctc
     4981 ccgggaattc acattgccgt ggaacatgtt caccgagggc gcctcccagc cgcgcgcgac
     5041 gcccttcgcg ggataccaca cggttgtcgt gagccgtcgt gcgcgcgcgg catcgtagta
     5101 cgcgcggacg gcgcgaccca cggagatggt ctccgcgcgc tcgatcttca cggcggaaga
     5161 acaaccggct ccgatgacgc cgatcgccac tgccatgaga gcgcagagaa cggttgcgcc
     5221 agtgccgatt cgcatgcgtg cctcgtgtat cgaaatcttc atcgccattg acgctgccgc
     5281 gatggattca ttctgtcaag aggaaagcgc gtcggcgaca tccgcccggc gcggcgcgcg
     5341 cggcagcgcg catcatcccc acgaagcata cttgacattt ccgggcggcg cgcataggat
     5401 caacgaaacg ccttccggga gaccatcgca tgaaaaaacg cctcttcgtc gtcgacggcc
     5461 acgcgctgtg ctaccgcgcg tatttcgcct tcatcaagaa cccgctggtg accgccgacg
     5521 gacagaacgt ctcggcgatc tacggcttcg ccctcatgct cctcaagctc atcaacgacc
     5581 agcggcccga ttatctcgcc gtcgcgttcg atccgccgcg caagtccttt cgcttccgca
     5641 tgtatcccga gtacaaggcc acgcgccaga aaatgcccga cgacctccgg agccagatcg
     5701 acgagatcaa gcacatgctc tccgtgctgg gcatcgttcg cgtcgaggag gaggacttcg
     5761 aggccgacga cgtgctgggc acgctcgcga cgacgcacgg atcgaagagc ctcgaggtgg
     5821 tgctcgtgac cggcgacaag gacgcctacc agctcgtcgg gccgcacgtg cgcatctacg
     5881 ccggaaagaa ggggatcacc gagttcgaca tctacgacgc gaaggccgtc gaggagaagc
     5941 tcggcgtcac accgacgcag gtgatcgact acatggcact caccggggac gcctccgaca
     6001 acgtccccgg cgtgaagggc gtgggcgaga agaccgcgct ccagctcatc accgactaca
     6061 aaacgctcga tggcgtctac gcgcacgtgg acgagatcac gggcaagctc aaggacaagc
     6121 tcgtcgagag caaggagatg gcctatctct cgcgcgacct ggtcaccatc cgcaccgacc
     6181 tccccctcca gttcgacatc gaacagtcgg cgctcccgga cctgaagacc ccgacggtgc
     6241 gatcgtactt cgcgcagtac gagatgaaat ccatcctcaa agagatcttc ggggacggcg
     6301 cgggggggga tgataatgcc gccgccgcgc ccgggaccga ccgcgcgacg cgggactacc
     6361 gcaccgtccg caccgccgac gagctggccg ccgcctgcgc ggcgatccgc gccgcgggcg
     6421 aggtctc
//
LOCUS       DAORHF010000209         6406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12073_length_6406_cov_4.761297, whole genome shotgun sequence.
ACCESSION   DAORHF010000209 DAORHF010000000
VERSION     DAORHF010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12073_length_6406_cov_4.761297"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PKJ16_14505"
     CDS             <1..583
                     /locus_tag="PKJ16_14505"
                     /inference="COORDINATES: protein motif:HMM:NF018449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HNV48253.1"
                     /translation="GLQYYRFSSTCTKLNWVAGFQSGPQLGASIGSIATNQEDGTQYV
                     YIVGGSKATTFCGQPMPNPAHTRHDAYIAKIGLDGTTVWCRRFGSTDDLLDRTSGIEI
                     INNSIYVSGVIGSLPSTTSDAFIAKYDLGGICEWTRYASGAGADNASGLSAKNDMNIF
                     ISGTTDSNPFYGNANVGGTDCFILRYNHKGDMY"
     gene            917..1348
                     /locus_tag="PKJ16_14510"
     CDS             917..1348
                     /locus_tag="PKJ16_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV48254.1"
                     /translation="MKKSSIILIALALVVATAAAGYADIQIRKGGSMWAEVSSSGTIR
                     INGSIVGEFSSNGTVRKSGSIVGEVESGGTIRKSGSIVGQIESNGTFRKSGSIIGAIE
                     SGGTIRKGGSIWGSASSCCGDAYARRAVAAVLVFFSGDFGF"
     gene            1445..2044
                     /locus_tag="PKJ16_14515"
     CDS             1445..2044
                     /locus_tag="PKJ16_14515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV48255.1"
                     /translation="MIHEQFTQYRKSLLFLDSLPYPVIIHHNLAIVYVNRQGLEVIEH
                     SRGKKIMGRNIFDFILPHFHEKVRARLNEHDRGMPFLSHTVETLVTFSGRRITLELTS
                     SLIKLGRKKCIMVIGKPVTTSGKSAFHAGDWATTGRSSKSTGGRPLVSMCASCKGVKS
                     AAGGWVPVELFVAAIAPLAFTHGICPRCAKSLYGLDIEE"
     gene            complement(2094..2762)
                     /gene="hypB"
                     /locus_tag="PKJ16_14520"
     CDS             complement(2094..2762)
                     /gene="hypB"
                     /locus_tag="PKJ16_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HNV48256.1"
                     /translation="MEIKVLQDIFESNRNMADEIAALLKEKKVYMIDLMSSPGSGKTT
                     ITDRLIGALKGSFRIAVIEGDIKTTKDAEKLAKHDIPIIQIETARFSGDCHLESSWIR
                     KCLDGFDLDNLDLVIIENIGNLVCPAEFQLGDDERIVVLSVTEGEDKPVKYPLMFNSS
                     HTLLLNKIDLLPHLEYSMDEVMENVRRTNPTIVTFPVSAKTGEGFDAVVAHLARRIRE
                     KTGK"
     gene            complement(2773..3336)
                     /locus_tag="PKJ16_14525"
     CDS             complement(2773..3336)
                     /locus_tag="PKJ16_14525"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HNV48257.1"
                     /translation="MDAQRFILEAGRCCLLVVDIQERLYNAMDFNVRKSVVRNTNTLI
                     EVAKAFALPIVLTEQYRKGLGPTIPEIMNNLPGVEALDKLTFDCLGEGAIAARFDEIG
                     RDTVIIAGIETHICVLSTAFSLLAGGRRVAVASDAVCSRREYEREMAIEALWRAGAVV
                     YPTESIAFMLMRKSGTEEFKTLSPLFK"
     gene            3426..3962
                     /locus_tag="PKJ16_14530"
     CDS             3426..3962
                     /locus_tag="PKJ16_14530"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HNV48258.1"
                     /translation="MRTDGTRIIRHIAGYAVGIFLFLVLIPFGLYWLSVVTAPYPPLA
                     VTGHEAARAVISIPLFVAGIVFMAWSNIDLFRIGKGGPTDGLGVTISPRSQHLVVVGP
                     YRYCRNPMVFGAYALYFAIAAALGSVAGTLVLALFVGISIIYLKHTEERRLERDFGDE
                     YRAYRRRVSMLLPLPPKK"
     gene            4025..4447
                     /locus_tag="PKJ16_14535"
     CDS             4025..4447
                     /locus_tag="PKJ16_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006040562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HNV48259.1"
                     /translation="MIENYFHNCLYFTASTLSRAITRIAEEEFRTTGLSPTYAFLILI
                     VIDRPGISQKELGEILHITQSTITRFIDKLESEKFVTRQSSGKSSLIHPTDRGRAARP
                     DIEKAWKRLYDRYTKALGQKDSEKLTTLTDRAGLLLEK"
     gene            4518..5084
                     /locus_tag="PKJ16_14540"
     CDS             4518..5084
                     /locus_tag="PKJ16_14540"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HNV48260.1"
                     /translation="MKKISLGSNIFEFPMPVTLLGTSVGGRANFMALGWISRANANPP
                     LVVIGVGNHHYSIRGLRESKTFSINYPGADMRIATDYCGIVTGESTDKSKVFDVYYGV
                     LKTAPLIAECPLAFECRLVQEVPLQSNSLFIGEIVESYTEERYLTAGKPDIKKMNPLL
                     LTMPDNRYWTVGECAGAAWHDGREYKRS"
     gene            complement(5118..5687)
                     /locus_tag="PKJ16_14545"
     CDS             complement(5118..5687)
                     /locus_tag="PKJ16_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48261.1"
                     /translation="MMELNIGRMLMELLAAAIIQFTPVDSYFETNRTWTWGDTLSRWY
                     VLEPSRQGEYAVFTFRRFEGEVRVADSIVLTARRGRDGKYAWMTFRRDYDKSARAFVA
                     PLPDNAGNIGAVTRFTPAVWESSAANIPLKQKGAPTVDGVPCTQLLFSDGLISVNLVL
                     GRPGLREMTIVAPPGGPGGGENRLRLENP"
     gene            complement(5854..>6406)
                     /locus_tag="PKJ16_14550"
     CDS             complement(5854..>6406)
                     /locus_tag="PKJ16_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48262.1"
                     /translation="AHFTGPKPDGSGRYHLYYVYSQQYLTGKTYEEWDWGCWCYVERT
                     REYGPYANNNFANWNYKDYCILDVDDRFRNGPETVTVKQPIYLGSLADKVYRYAVHDY
                     SNRDANLSKALSKSGAIVTVKRYDLLVNGSPLTYTFNIPYDKVGTVWNVFEMTYNEAY
                     QGKFVILPKNTFRNESTSSNVIQ"
BASE COUNT         1305 a         1964 c         1772 g         1365 t
ORIGIN      
        1 cggattgcag tattatcgat ttagtagtac atgcactaaa ctcaattggg ttgccggctt
       61 ccagagtggc cctcagcttg gagcaagtat tggttctatt gccacaaacc aagaagatgg
      121 gacccaatat gtatacatcg tcggcggttc gaaagctaca actttttgcg gccagcccat
      181 gccaaacccg gcacataccc gccatgatgc ctatatagcc aaaattgggc tcgatggaac
      241 cacggtatgg tgcaggcgct ttggctctac cgatgactta cttgaccgga cttccggaat
      301 cgagattata aacaactcga tttacgtttc cggcgtgatt ggctcgttgc caagcaccac
      361 aagcgatgcg tttatagcca aatacgatct tggcggcata tgcgagtgga cccgctatgc
      421 tagcggcgca ggtgccgata atgcaagtgg cctgagcgcc aaaaacgaca tgaatatctt
      481 catctccggc actaccgact caaatccgtt ttatgggaac gccaatgtgg gaggcacgga
      541 ttgtttcatt ctccgataca accacaaagg cgatatgtac tgatggatta ttccatgcga
      601 tattcatcac aaggcggggt tgattcccgc cttgttcggt tttaccgttt gaaaccattc
      661 cagacctccc tctccttgcg ggagcggtgg aacgggagga aggtatccgg ggagtcagag
      721 cccctcaatt ttaccacagc ccaaatatcc ttgacatcct ccatttcccc tgctaccgat
      781 agttatcggt tttatctcac taattgaagc cattttccac acgccccccc actatcggga
      841 acaatgggct ggagagcgag gtttctcggg gtcagcgccc caatcgcacg ctctatttta
      901 ccaactggag gtacacatga aaaaaagctc catcattctc atcgccctgg cgctggtggt
      961 cgcgaccgca gcggcgggct acgccgacat tcagatccgc aagggcggct cgatgtgggc
     1021 cgaggtgtcg tccagcggca ccatccgcat caacggcagc atcgtgggcg agttctcgtc
     1081 caacggcacg gtgcgcaagt ccggcagcat cgtcggcgag gtggagtccg gcggcaccat
     1141 ccgcaagtcg ggctccatcg tgggacagat cgagtcgaac gggacgttcc gcaagtcggg
     1201 cagcatcatc ggcgcgatcg aatccggcgg aaccatccgc aagggcggct ccatctgggg
     1261 atcggcgtcc agctgctgtg gagacgcata tgcccggcgc gccgtggccg cggtgcttgt
     1321 ctttttttcc ggcgatttcg gattctagcc ggttttttta gaaaaaaaag cccggaacgc
     1381 gatacaatcg cgaaatccgg gttttttttt gtcaatttca ggcccgtaac gacgctctat
     1441 attcatgata cacgaacagt ttacacaata tcggaagtcg ctcctcttcc tggacagcct
     1501 tccgtatccg gtcatcatcc accacaacct cgcaatcgtg tacgtgaacc ggcagggcct
     1561 ggaggtcatt gaacactcgc gcgggaaaaa aatcatgggg cgcaacatct tcgatttcat
     1621 cctgccccac ttccatgaaa aggtgcgcgc acggctcaat gaacacgacc gggggatgcc
     1681 cttcctctcg cacacggtgg agaccctggt gacgttttcc gggcgccgca tcacgctcga
     1741 gctgaccagc agcctcatca agctcgggcg gaagaagtgc atcatggtta tcgggaagcc
     1801 cgtcacgacg tcggggaagt ccgcattcca cgccggggac tgggccacga ccggccgttc
     1861 gtcgaagtcg acgggcggcc gtccgctcgt ttccatgtgc gcctcgtgca agggggtgaa
     1921 aagcgccgcg ggcggatggg tgccggtgga gcttttcgtc gcggcgatcg cgcccctggc
     1981 cttcacccac ggcatctgcc cgcgctgcgc gaagagcctc tacggcctgg atatcgagga
     2041 gtaagcgtta cgcgcgatcc atgcgcgcgc atgcgcgagg gaagcgaacg tctctacttc
     2101 cccgtcttct cacgtatccg ccgtgcaaga tgggcgacaa cggcgtcgaa gccctcgccc
     2161 gtcttcgcgc tcacggggaa ggtgacgatg gtcgggttcg tccgtcgcac gttctccatc
     2221 acctcgtcca tgctgtattc gaggtgcggc agcaggtcga tcttgttcaa cagcagcgtg
     2281 tgcgacgagt tgaacatgag cgggtacttg accggcttgt cctcgccctc ggtcacggag
     2341 agcaccacga tgcgctcgtc gtcgccgagc tggaactccg cggggcacac gagattgccg
     2401 atgttctcga tgatcacgag gtcgagattg tccaggtcga acccgtcgag gcacttgcgg
     2461 atccacgagc tttcgaggtg gcagtcgccg ctgaagcgcg ccgtctcgat ttggatgatg
     2521 gggatgtcgt gttttgcgag cttctcggcg tccttggtgg tcttgatgtc gccctcgatc
     2581 accgcgatcc ggaagctccc cttgagcgcg ccgatgaggc ggtcggtgat ggtggtcttc
     2641 cccgatcccg gcgagctcat caggtctatc atgtagacct tcttctcctt caagagggcc
     2701 gcgatctcgt cggccatgtt gcggttcgac tcgaagatgt cctgcaatac cttgatttcc
     2761 atcggcccct ccttatttga acagcggcga aagcgtcttg aattcctcgg tccccgattt
     2821 cctcatgagc atgaaggcga tcgactcggt gggatacacc accgcgcccg ctcgccagag
     2881 cgcctcgatc gccatctcac gttcgtattc ccggcgcgag cacaccgcat cgctggccac
     2941 cgcaacgcgc cgcccgcccg caagcagcga gaacgccgtg gagagcacgc agatgtgcgt
     3001 ctcgatcccc gcgatgatca ccgtatcgcg gccgatctcg tcgaagcgcg cggcaatggc
     3061 cccctcaccg agacagtcga aagtgagctt gtccagcgcc tcgacgccgg gcaggttatt
     3121 catgatctcc gggatggtgg gacccagccc ctttcggtac tgctccgtga ggacgatggg
     3181 gagcgcaaag gccttcgcga cctcgatgag cgtgttcgtg tttcgcacca cgctcttacg
     3241 cacattgaaa tccattgcat tgtacagccg ctcctggatg tccacaacca gcaggcagca
     3301 acgccccgct tcgagtataa agcgctgtgc gtccatcggc gcccccctaa cacgatttgt
     3361 gttgacagag tccgcgtgcg gcataaaaat ttaaacactt ttttcaacac gcagggggcg
     3421 cgatcatgcg aaccgacggc acacggatca tccggcacat agccggctat gcggtgggaa
     3481 ttttcctctt tctcgtgctg atccccttcg ggctctactg gttgtcggtc gtcaccgcgc
     3541 cgtatccgcc cctggccgtc acgggccacg aggccgcgcg ggccgtcatt tcaataccgc
     3601 tttttgtcgc gggcatcgtc ttcatggcct ggtccaacat cgatcttttc cgcatcggca
     3661 agggagggcc caccgacggc ctgggcgtca ccatcagtcc gcgttcgcag cacctggtcg
     3721 tggtcgggcc gtaccgttac tgccgcaacc cgatggtctt cggcgcctac gcgctctatt
     3781 tcgcgatcgc cgccgcgctc gggtcggttg ccggcacgct cgtcctggct ttgttcgtgg
     3841 gcatctccat catctatctc aagcacaccg aggagcggcg gctcgagcgc gacttcggcg
     3901 acgaataccg cgcctaccga cggcgggtgt cgatgctgct gccgctcccg ccgaaaaaat
     3961 aggaaaaacc gttgactccc cgccctgctc atgcttgtat gtacatgcat taatggtcat
     4021 tgccatgatt gagaactact ttcacaattg tctctacttt acagcgagca cactctcgcg
     4081 ggcgatcacc cgtatcgcgg aagaagagtt ccgaaccacc ggcctctccc cgacctacgc
     4141 ctttctgata ctgatcgtca tcgatcggcc ggggatcagc cagaaggaac tgggggaaat
     4201 acttcacatc acgcaatcga ctatcacccg cttcatcgac aagctcgaaa gcgagaaatt
     4261 cgtgactcgc cagagctcgg gtaaaagctc gctcattcac cccaccgacc gcggaagggc
     4321 ggcccggccg gatatcgaaa aggcctggaa gcgattgtac gatcggtaca cgaaggccct
     4381 ggggcagaaa gacagcgaaa agctaacgac cctaaccgat cgggccggtc tactccttga
     4441 gaaatgagcc ttttttattc tgcgcacata tttgcatgta catacattat ataccactga
     4501 acaccatgga ggctttcatg aaaaaaatct cgcttggatc aaacattttc gaattcccca
     4561 tgccggtcac actcctcggc acttccgtcg gggggcgcgc caacttcatg gcgctgggct
     4621 ggatctcgcg cgcgaacgcc aatccgccgc tcgtcgtcat cggcgtgggg aaccaccact
     4681 acagcatacg gggcctgcgc gaatcaaaga ccttcagcat caactatccc ggcgccgata
     4741 tgcgcatcgc gaccgattac tgcggcatcg tcaccggcga atcgaccgac aagtcaaagg
     4801 tcttcgatgt gtactacggt gtgctgaaaa ccgcgccgct catcgccgag tgccccctgg
     4861 ctttcgaatg ccggttggtg caggaggtgc cgttgcaatc gaactcgttg ttcatcgggg
     4921 aaatcgtcga gtcatacacg gaggagcgtt accttaccgc cgggaaaccc gatatcaaga
     4981 agatgaaccc gctgctgctc accatgcccg acaaccgcta ctggacggtg ggagaatgcg
     5041 ccggcgccgc gtggcacgac gggagagagt ataagcgctc ctgaagcgat gatccccgac
     5101 gggcgggcgc accgccctca ggggttctcc agcctgagcc ggttctcgcc accgccgggt
     5161 ccgccgggcg gcgctacgat ggtcatctcg cgtaggccgg gcctgccgag caccaggttc
     5221 acggagatca atccatcgga gaagaggagc tgggtacagg gaacgccgtc cacggtcggc
     5281 gcgccctttt gtttaagcgg aatgttcgcg gcgctgctct cccacacggc cggcgtgaag
     5341 cgagtgacgg cgccgatgtt cccggcattg tccggaagcg gtgcgacaaa ggccctggcc
     5401 gatttatcat agtcccgccg gaaggtcatc caggcatatt tcccgtcccg cccccggcgc
     5461 gccgtgagga cgatgctgtc cgcaacgcgg acctcgcctt cgaaacgccg aaatgtgaac
     5521 acggcatact cgccctgccg ggacggctcc agcacatacc agcggctcag ggtatcgccc
     5581 caggtccagg tgcggttggt ctcgaaatac gaatcgaccg gcgtaaactg aatgatcgcg
     5641 gccgccagca gctccatcaa catcctgccg atgttcagtt ccatcatggc tcctccccgg
     5701 gcgggtacgt tcgtctcgtg cgttttgctc gcctacaaga tactgcaccg actctccccg
     5761 gtcaaccgga taaatgctcc catgccgggc gcgcatacga aaggccgggc atgttgcccg
     5821 gcctttctga atcatgcgga ttaaactgtc agcctattgg atcacattcg atgaagtcga
     5881 ctcgttcctg aacgtgtttt tcggaagaat cacaaacttg ccctgatacg cttcattata
     5941 cgtcatctcg aagacattcc ataccgttcc aaccttgtcg tacggaatat taaaggtgta
     6001 ggtgagcgga ctgccgttca cgagcaggtc gtagcgcttc acggtcacga tcgccccgga
     6061 cttcgaaagc gccttgctta aattcgcgtc tctgttcgaa taatcatgaa ccgcataccg
     6121 gtacaccttg tccgcaagcg aaccaagata tatcggctgc tttacggtaa ccgtctccgg
     6181 cccgttcctg aagcgatcgt caacatccag gatgcagtaa tccttgtagt tccagtttgc
     6241 gaaattatta tttgcatacg ggccatactc ccgtgttcgt tccacatagc accagcaacc
     6301 ccaatcccat tcctcatatg tctttcctgt caggtactgc tgtgaataga catagtatag
     6361 gtgataccgc ccgctcccat ccggcttcgg gccggtgaag tgggca
//
LOCUS       DAORHF010000210         6360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12206_length_6360_cov_4.550198, whole genome shotgun sequence.
ACCESSION   DAORHF010000210 DAORHF010000000
VERSION     DAORHF010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12206_length_6360_cov_4.550198"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PKJ16_14555"
     CDS             <1..390
                     /locus_tag="PKJ16_14555"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNV48263.1"
                     /translation="AHELHVGLVGVAPALFADRVTSFVRLPFPNFDRHEQAIDAPVAH
                     VDRFGNVALAIPNAVLSEIDGAAWEIILGDEGVPAEPVRTYADIPEHGIGILPGSSGF
                     VEIAMREESLAETFGVNIEDRIRIRYE"
     gene            383..1606
                     /locus_tag="PKJ16_14560"
     CDS             383..1606
                     /locus_tag="PKJ16_14560"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HNV48264.1"
                     /translation="MSSNRKIAVLIGDGMADEPIAALGGKTPLEAARTPNMDSLARRG
                     TFGLARTIPPGMDPGSDTANLSIMGYDPRVYFTGRAPLEALNMGIELGRDDVAFRCNI
                     VRIDESGTMVDFSGGHIESDFGKVVIDELRAARAFPGIELYSGVSYRHLLVWRGYPHS
                     SLPATTPPHDIQGKDSAPYLPAGDGAGLLREIMDLSRGVIASSKRIADAAARMRGAPT
                     SVWLWGGGRKPSMPTLAERFGITGRTISAVDLIHGLGRAAGLTPLRVPGATGYIDTNY
                     EGKADALIAAFETDDFVFLHVESPDESGHEGNLEHKLRAIEDFDARVVGRVLAGMARY
                     DDYAVLVMPDHPTPIAIRTHSAAPVPFCIFDAKRDGASRDGVRGFSEPEAARTGIVVE
                     DGHRLIEHLIAGHIG"
     gene            1622..3109
                     /locus_tag="PKJ16_14565"
     CDS             1622..3109
                     /locus_tag="PKJ16_14565"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HNV48265.1"
                     /translation="MKLAVKAAIAVVIALALVAVGIKLFKGKSVDTAIGYYEDGDYDD
                     AIRLLNRLVGTLDYEQAEKAYYYRCRSINRYAEKLERKYDDELSVLGEQAGDAKPERI
                     RDYREKLTRKLASINESIDGDLTLAAGRSRGRIVSGGKFYNQFLAKYKGSRFLEDLDF
                     EELQKIERTEGHRLLRSIANFHARYPSTTYLAQVVKMLFDALKRGEVQIKEKDDFLAN
                     LIIQFGRKYPTSSEFSRLFVCNSESVNMRNSPDTRGALVAKIDKGEILIQLEKSMDTF
                     QVGDVRDYWYRVATLRGHAGWIFGKFLAPIDMSRFKDVETEERWSLDERFADWTDSHT
                     PKNWMHVDERGSGAVSFSSREGANRLVFNASHAGGAPAGLYRKAPAGRSFVILARARF
                     AGGSPVTLFANSMPGEKVFAVVIGDEEVEVSGRRIPLRTGEWHEYRLESEDGAFARLI
                     VDGEVISSRINPVRNAAYRSTGIYCLFAPAGVIGAAEMEYIKLRN"
     gene            complement(3316..4002)
                     /locus_tag="PKJ16_14570"
     CDS             complement(3316..4002)
                     /locus_tag="PKJ16_14570"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HNV48266.1"
                     /translation="MKKTALIAIALLLCAGPLFAQDKKAPDAKKDDKSATDTKEAKDV
                     DRGGYAEMVLEDFETTSYTDKSISFRVTKDQKAGVAVRDQFPAPTPGSKKYLGVKFYS
                     KRGDVLTIYPAKKLIIEKYCRSLSVWVYGKNFSGELSMLLRDGTGQNHRLVFGKLNFQ
                     GWKKMSVKITDRIPQEDKYLSQKRFLEITQIIYAPGNTTSLPIWNYFYLDDISASVRE
                     KYIDKQSDEW"
     gene            4229..5884
                     /locus_tag="PKJ16_14575"
     CDS             4229..5884
                     /locus_tag="PKJ16_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48267.1"
                     /translation="MSRTPVTLIRSLIFIAVAIVSLRSGAFGAQSYDWWWVFYEHETT
                     KRFVSDTVRPFFLRNTYGDRVFEASLMPIVYWKYKTPRTTEWKSLFGLVDSVDHRHRS
                     GLHDYDFAFFPFLYWGHSSDQKENYFMFWPFGGRVYNKLGQDRIDTVLFPGFALFFLY
                     PPTWPVSWTTLGILVLSFIPLYVDYENRQYQAWGILWPLIQRGANPKRDDVRVLPFYA
                     HSYKKDRYDAYNVLLLFNYTKVYLRDDVQKTLFVLPFYGRRWNESDKVNSSTLLWPFF
                     SWGYDRGSGAFEVNFPWPLVQYHDSLSPFVKKRIFFPFYGLYEREHQRTRFITPLYIS
                     LQKFNRRLESEYNFYALIFWHFKRDYKLASSPVYGKSWRYWKLWPLFQYEYDDRGNVA
                     FNMLSILPFRDPDGYERLYQPFWTLFEYRRLADGEKRLGLLLRLYYQRWGDDFLYIKV
                     PFLISYGRAHGRITELSVLLSMFAYINDERGRYVRLFWIPVGLGGAAIAVDGAADTPT
                     LGATAWAPGGGFDDDIVFHRGVEGFDALSRDRANLYGYRHAVF"
     gene            6024..>6360
                     /locus_tag="PKJ16_14580"
     CDS             6024..>6360
                     /locus_tag="PKJ16_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV48268.1"
                     /translation="MSIKFIHSFFGLFGYVGQRIVDTIELTGYTLVLLLQAGFYTKNV
                     FSKRREILKQMYIAAVKTFFVVSVVALFTGMIVALQTGIEMKAFKQEALIGNVIIATM
                     TREMGPFMTA"
BASE COUNT         1171 a         1879 c         1995 g         1315 t
ORIGIN      
        1 gcccacgaac tgcatgtcgg gctggtaggt gtggcgccgg ccctcttcgc ggaccgcgtg
       61 acgtcgtttg tccgcctgcc cttcccgaac ttcgatcggc atgaacaggc gatcgacgcg
      121 ccggtcgcgc atgtcgatcg ctttggaaat gtcgcgctcg cgataccgaa cgcggtgctt
      181 tcggaaatcg acggcgcggc gtgggagatc atcctcggcg acgagggcgt tccggcggaa
      241 ccggtgcgga cctatgcgga cattcccgaa cacgggatcg ggattctccc ggggagctcg
      301 ggtttcgtcg agattgcgat gcgcgaggag tccctcgccg aaacatttgg cgtaaacatt
      361 gaagacagga tacggatacg ctatgagtag caaccggaag atcgcggtcc tcatcggcga
      421 cggcatggcc gatgagccca tcgcggcgct gggcggcaaa accccgctcg aggccgcccg
      481 gacgccgaac atggactcgc tggcgcggcg gggcaccttt ggcctcgcgc gcaccatacc
      541 gccggggatg gacccgggga gcgacacggc gaacctgtcc atcatgggct acgacccgcg
      601 ggtgtacttt accgggcggg cgccgctcga ggcgctcaat atgggcatcg aactcgggcg
      661 cgacgatgtg gccttccgct gcaatatcgt acggatcgac gagagcggga ccatggtcga
      721 tttcagcggc gggcacatcg agtccgattt cggcaaggtg gtgattgacg agctccgcgc
      781 cgcgcgtgcg tttcccggga tcgagctcta ttcgggcgtt tcgtatcggc acctgctggt
      841 gtggcgcggc tatccgcatt cgtcgctgcc cgcgacgacg ccgccgcacg atatccaggg
      901 caaggacagc gcgccgtatc ttcccgccgg cgacggggcg gggctcctac gggagatcat
      961 ggacctctcg cgcggagtga tcgcatcgtc gaaacggatc gccgatgccg ccgcgcgcat
     1021 gcggggcgcg cccacgtccg tgtggctctg gggcggcggg cgcaagccgt ccatgcccac
     1081 gctcgccgag cgcttcggca tcacgggtcg caccatatcg gcggtggacc tcatccacgg
     1141 gctgggacgc gcggccgggc ttacgccgct gcgcgtgccg ggggccaccg gctatatcga
     1201 cacgaattac gaggggaagg ccgatgccct catcgccgcg tttgagacgg acgatttcgt
     1261 gttcctgcac gtggagtcgc ctgacgagtc cggccacgag ggcaatctgg agcacaagct
     1321 gcgcgccatc gaggatttcg acgcgcgcgt ggtgggccgc gtcctcgcgg gcatggcgcg
     1381 atatgacgat tacgccgtcc tggtgatgcc cgaccatccc acgccgatcg ccatccggac
     1441 gcactcggcc gcgccggtgc cgttttgcat cttcgacgcg aaacgcgatg gcgcatcacg
     1501 cgacggtgtg cgcggcttct ccgagcctga ggcggcgcgc accggtatcg tggtggagga
     1561 cgggcaccgg ctcatcgagc atctcatcgc ggggcatatt gggtaacacg gaggatcgga
     1621 catgaaactc gcagtaaagg cggccatcgc ggtcgtgatt gcgctggcgc tggtggccgt
     1681 agggatcaaa ctgttcaagg ggaagtccgt cgataccgcc atcgggtatt acgaggacgg
     1741 cgattacgac gacgccatcc gcctgctcaa ccggctcgtc gggaccctcg attacgaaca
     1801 ggccgagaag gcgtactatt accgctgccg ctccatcaac cgctatgccg agaagctcga
     1861 gcgcaaatac gatgacgaac tgtcggtcct gggcgagcag gcgggcgacg cgaagccgga
     1921 gcgcatcagg gactaccgcg agaagctcac gcgcaagctg gcgtcgatca acgagtcgat
     1981 agacggcgat ctcacgcttg ccgccggtcg ctcgcgcggg cgcatcgtct ccggcggaaa
     2041 gttctataac cagtttctgg cgaagtacaa gggcagccgt tttctcgagg acctcgactt
     2101 cgaggagctc cagaagatcg aacgaaccga ggggcatcgt ctccttcgct cgatcgcgaa
     2161 ctttcacgcc cgctatccct ccacgaccta tctggcgcag gtggtgaaga tgctcttcga
     2221 cgccctgaag cgcggcgagg tgcagatcaa ggaaaaggac gattttctcg cgaacctcat
     2281 catccagttc gggcggaaat atccgacgag ctccgagttc agccgcctct tcgtgtgcaa
     2341 ctcggagagc gtgaacatgc gcaattcgcc ggacacgcgg ggggcgctgg tggcgaagat
     2401 cgacaagggg gagatactca tccagcttga aaaatcgatg gacaccttcc aggtcgggga
     2461 cgtgcgcgat tactggtacc gcgtcgcgac gctgcgcggc cacgccggat ggatcttcgg
     2521 gaagttcctc gcgcccatcg acatgtcccg cttcaaggac gtggagacgg aggagcgctg
     2581 gtcgctcgac gagcggttcg ccgattggac cgactcgcat acgccgaaaa attggatgca
     2641 cgtggacgag cgcggatccg gcgcggtcag tttttcctcc cgggagggcg ccaaccggct
     2701 ggtcttcaac gcctcgcacg cgggcggcgc gccggcggga ctgtaccgca aggcgccggc
     2761 ggggcgatcg ttcgtcatcc tcgcccgggc ccggttcgcc ggcggatcgc cggtcaccct
     2821 gttcgccaac agcatgccgg gcgagaaggt cttcgccgtg gtcatcggcg acgaggaggt
     2881 cgaggtgtcc ggccggcgca ttccgctgcg caccggggaa tggcacgagt accgtctcga
     2941 gagcgaggac ggcgcattcg cgcgcctcat cgtcgatggc gaggtgatat ccagccgtat
     3001 caatccggtc cgtaacgccg cgtaccggtc aaccgggatc tattgcctct tcgcgccagc
     3061 gggcgtcatc ggcgccgcgg agatggagta tatcaagctg cgcaattagg gatcgattcg
     3121 ggcgcgcacc cgattgccgc ctcattcgga cgccatgagc cgggtgacga gtggtattgg
     3181 ctctcgggaa ggcgtcgttg tcgtatttgt tgatgcgcaa tacggccatg tgagggaatt
     3241 gcgcctggcc tggaaaacac gaacggcggg tcatggtgac ccgccgtttt tcgcggcacg
     3301 ggtgatcggt ttcgcctacc actcgtcgct ttgcttgtcg atgtactttt cgcgaacgct
     3361 ggcgctgatg tcgtcaagat agaaatagtt ccagatcggc agcgacgtgg tgttcccggg
     3421 cgcgtagatg atctgggtga tctccaggaa gcgcttctgc gagaggtact tgtcctcttg
     3481 cgggatgcgg tcggtaattt tcacgctcat ctttttccag ccctggaagt tgagcttccc
     3541 gaacaccagg cggtggttct ggccggtgcc gtcgcggagc aacatggaaa gctcgccgga
     3601 gaagttcttc ccgtataccc ataccgacag gctccggcag tatttttcga taatgagctt
     3661 tttcgcggga tagatggtga ggacatcgcc gcgcttcgag tagaatttca cgccgaggta
     3721 tttcttcgag cccggggtcg gcgcgggaaa ctggtcgcga acggccacgc ccgccttctg
     3781 gtccttggtc acgcggaacg agatgctttt atcggtgtag gaggtcgttt cgaagtcctc
     3841 aagcaccatc tcggcatagc cgccgcgatc gacatccttc gcctctttgg tgtcggttgc
     3901 ggacttgtcg tcctttttcg cgtcgggggc cttcttgtcc tgcgcgaaga ggggcccggc
     3961 gcacaacaga agcgcgatcg cgatcaatgc cgtctttttc atacaacaac ctccgtttgt
     4021 gataatcaat acatgtataa tgggcatata tcgacatgtc aaatataaat aattatgaaa
     4081 ataccataaa aaagccgttg ggagtatcgt cccatgtcgg cggcgaatcc gcccggggga
     4141 atgcgttcca cgggaaatta ttcttgacgc gtgaattttc cacacgccta ctctcgtcga
     4201 tgcggaataa ttctcgacgg tgcctccggt gtcacgaacg ccagtaacgc tcatcaggtc
     4261 ccttatcttc atagcggtcg ccatcgtgtc gctgcgttcc ggcgcgttcg gcgcgcaatc
     4321 ctatgactgg tggtgggtct tctacgagca cgaaacgacg aagcgtttcg tgagcgatac
     4381 cgtgcggccg tttttcctgc gcaacaccta tggggaccgg gtgttcgagg cgtcgctgat
     4441 gccgatcgtc tactggaaat acaagacccc ccgcaccacg gagtggaaat cgcttttcgg
     4501 gctggtcgat tcggtggacc atcgacatcg cagcggtctc cacgattacg atttcgcctt
     4561 cttccccttc ctgtactggg ggcattcctc ggaccagaag gagaactatt tcatgttctg
     4621 gcccttcggc gggcgggtgt acaacaagct cgggcaggac cgcatcgata cggtgctgtt
     4681 tccgggattc gcgctctttt tcctctaccc gccgacctgg ccggtgtcgt ggacgacgct
     4741 ggggatactc gtgctgtcgt ttatcccgct gtacgtcgac tatgagaacc ggcaatacca
     4801 ggcctgggga atcctgtggc cgctcatcca gcggggcgca aatcccaagc gcgacgacgt
     4861 gcgcgttctt ccgttttacg cgcacagcta caaaaaggac cggtacgatg cgtacaacgt
     4921 actccttctt ttcaactata cgaaggtgta cctgcgtgat gatgtgcaga agaccctctt
     4981 cgtgctgccg ttttatggcc ggcggtggaa cgagtccgac aaggtcaatt cgagcacgct
     5041 cctgtggccg ttcttctcgt gggggtacga tcgcggcagc ggcgccttcg aggtcaattt
     5101 cccatggccc ctggttcaat accacgactc cctgagtccc ttcgtgaaga agcggatatt
     5161 tttccccttc tatgggctct atgagcggga gcatcagcga acgcgcttca tcacgccgct
     5221 ttacatttcg cttcagaaat tcaatcggcg gctcgagtcc gaatacaact tctacgcatt
     5281 gatcttctgg catttcaagc gggactacaa gctcgcgtcc agtccggtgt atggcaaatc
     5341 atggcgatac tggaagctgt ggccgctgtt ccagtacgag tacgacgatc gcggcaacgt
     5401 cgccttcaac atgctctcca tcctgccatt tcgggacccg gacggctacg agcggctcta
     5461 tcagccgttc tggacgctct tcgaataccg tcgcctcgcc gacggcgaga agcgtctggg
     5521 attgctgttg cgtctgtact atcagcgatg gggtgatgat ttcctctaca tcaaggtgcc
     5581 cttcctgatc tcatacggcc gcgcacacgg gaggatcacc gagctgtccg ttctcctgtc
     5641 gatgttcgcg tatataaacg acgagcgcgg gcggtacgtg cggctgtttt ggattcccgt
     5701 gggcctggga ggcgcggcga tcgccgtcga tggggccgcg gatacaccga cgctcggcgc
     5761 gacggcctgg gcgcccggcg gcggtttcga tgacgatatc gtcttccacc gcggcgttga
     5821 aggtttcgat gcgctgtcgc gtgaccgcgc gaacctgtac ggctatcggc atgccgtttt
     5881 ttaacttgca aataaccgtg cggatgatac gaatatcata ccggttcgtc cggacgcgga
     5941 tgggatgcgc atttgaacac aatattcatc tttcttttat gatatgtcaa catgcacccg
     6001 gcgtcgatga aatgtaacgc ggaatgagca tcaagtttat acacagcttt ttcgggctat
     6061 tcggctatgt ggggcagcgg atcgtcgata cgatcgagct gacgggctac acgctcgtcc
     6121 tcctgcttca ggcgggattt tacacgaaga acgtcttcag caagcgccgc gagatactca
     6181 agcagatgta tatcgcggcc gtgaagacgt tcttcgtggt gtccgtcgta gccctcttca
     6241 cggggatgat cgtggcgctg cagaccggca tcgagatgaa ggccttcaag caggaggcgc
     6301 tcatcggcaa cgtgatcatc gccaccatga cgcgcgagat ggggcccttc atgaccgcga
//
LOCUS       DAORHF010000211         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12291_length_6335_cov_4.073885, whole genome shotgun sequence.
ACCESSION   DAORHF010000211 DAORHF010000000
VERSION     DAORHF010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12291_length_6335_cov_4.073885"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="PKJ16_14585"
     CDS             <1..424
                     /locus_tag="PKJ16_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV48269.1"
                     /translation="IVAILKRMEDYRDRLVVIVAGYTDEMKRFIESNPGLKSRFSRYF
                     YFDHYKPDDLMKIFDISCTNVRFTVTDTARKKLAAQLEALYEKRDRSFGNGRLVRNIF
                     EKIVERQANRIAGITPLTDEILCTLTEEDIPEKIGLAD"
     gene            679..2256
                     /locus_tag="PKJ16_14590"
     CDS             679..2256
                     /locus_tag="PKJ16_14590"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HNV48270.1"
                     /translation="MRSVQIVISMILLFRMLSPAAAQQDADFTPERGGELIKVDMGDS
                     AAFMIGGNRVSLGGAIHMVLERNKDTLTGTYDAAMVDTMYKKFLRKYALNIDLEGGYK
                     YQKFPESLQMMSGEEQRSWDVSAALSKYFSSGTFVSAGIMHTYSKTEYIPINLMGNQI
                     TFGDPEYNRPVLFVSIQQELLKNSFGYNERRQKEILKNTAKMQREALVFQLAGLVVQT
                     VIDYWSVLVGNSGLENARLQLRETKRVRNITAQNVRLGLGESYDLNYYNTLVATSEGR
                     VAQAEQEYRDTLRRLLRTLNLESNLELSGTAVLSNKLPVINVEKSIETAYSKRADYKG
                     AQLMLDSARRELELYKNEDLPSVMAILKANTMGQRKEQSDAYEDAGKNKYPSWEARLK
                     VSYPLDNTEQKANKRNARYKLEQAKLQFDKYQRLVKDEVVSSVERIKTDYELYQKARE
                     ARVQAELHYRKILVNMRRGRVTNAVVKNALDAMIDSRQRELNALVQYNITLLRYDLAT
                     NFMFDRYKIDVNAYIPN"
     gene            2278..3957
                     /locus_tag="PKJ16_14595"
     CDS             2278..3957
                     /locus_tag="PKJ16_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HNV48271.1"
                     /translation="MFLPATPDEVARRGWERLDVVIVTGDAYVDAPQFGAAVIGRVLE
                     DAGYRVGIIAQPDIDSPRDITRFGEPALFWGVTAGAVDSIISNYTATGKRRNRDDLTP
                     GGVNDRRPDRASIAYANLIRRYFKNTAPIVLGGVEASLRRVAHYDARDNALRRSILFD
                     AKADYLAYGMAERTVLHLASAIKNGCDTRDISGLCHIAKQVPEGYIELPGFESCLSDK
                     DAFIAMHRLLAENMDSRSARGLVQRHGDRYLVHNPPPIDVSPEELDAIHELGYEREAH
                     PLCRARGEVRALDTVRFSITTHRGCFGGCSFCAIAIHQGRRIVSRSIESIVREARSFL
                     GHPSFAGIISDLGGPTANMYGMSCANDDAPVCAERQCAGISVCRTLVCTHERSIALLR
                     AVRSVPGIRKVFVASGVRHDLVLADHEFGRVYLEELVEHHVSGQLKVAPEHSEPAVLR
                     LMGKPDFDSLREFKQLFDDIVRAKRVPHYLTYYFIAAHPGSTDAHMRALHGKISRELR
                     ITPEQVQIFTPSPSTAATLMYYTGVNPSDGAAVFVERDRSRRERQKRIITG"
     gene            complement(3980..4267)
                     /locus_tag="PKJ16_14600"
     CDS             complement(3980..4267)
                     /locus_tag="PKJ16_14600"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HNV48272.1"
                     /translation="MDDDFVPVELTDEIDLHAFHPRDVKALLGDFIEHAIAKRYRSVR
                     IVHGKGQSVIKSIVIAFLENDPRVATFSDQAGNWGATIAQLIVPDDAPDRT"
     gene            complement(4277..5272)
                     /locus_tag="PKJ16_14605"
     CDS             complement(4277..5272)
                     /locus_tag="PKJ16_14605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV48273.1"
                     /translation="MKRFRPVPKALQTTSSEPSKAVRYLVILLLAGITIFVGGNLFVR
                     ATKERIVVNPQDYRLRDGFPVFTSADAYLKLVKTYRYNFGTTVTIHRIQAGESYWDIA
                     HRHNISLETIIGANPHLPSLAATEGLELVIPSENGVLFPIDDAADAVRMLTRVGNPLS
                     MRVKGDFRQGVFDLLSLDDIRFVFIKNAKPRIVGEALQDLYDIRMAFAVPVRGQFTSF
                     FGVRNDPIYKEATFHNGVDIRAPTGTPIRPLADGMVSRVGWNEGYGLSVFVLHRNGYE
                     TMYGHCSVIRVAQGDMVTKNDIIAEVGSTGRSTGSHLHFVVKRHGQLVDPLIFIW"
     gene            complement(5281..6096)
                     /gene="proC"
                     /locus_tag="PKJ16_14610"
     CDS             complement(5281..6096)
                     /gene="proC"
                     /locus_tag="PKJ16_14610"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HNV48274.1"
                     /translation="MQQTVNKRIACIGVGNMGGAILSRLASAQSTEMIYCYDIDAEKQ
                     AAAVKLYGIQGVSTINDLTEKSDIIIIAVKPDGVKKVLTEARDRIRNQTIISIAAGVS
                     IESITAVIGPDKKILRVMPNTPAKIGEGMIVISPHDTVGSDEIREATDIFGLLGRVAV
                     LPERMMDAVTAVSGSGPAYVFMLIQAMTDGAVKMGIPRKTALELAAQTAAGAARLVLE
                     AGADPIELRGQVTSPGGTTIDAVHVLERAGFSGIVMDAIEAAAKKSEALGRKS"
     gene            complement(6077..>6335)
                     /locus_tag="PKJ16_14615"
     CDS             complement(6077..>6335)
                     /locus_tag="PKJ16_14615"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HNV48275.1"
                     /translation="DLKNIDVRGLMTIGPFTDNIDTIRKSFRLLKNLLDDINSSYGMT
                     INELSMGMTSDYHVAIEEGATMIRIGTAIFGQRDYDATNRQ"
BASE COUNT         1342 a         1810 c         1862 g         1321 t
ORIGIN      
        1 gatcgtcgcg atcctcaagc gcatggagga ttatcgcgat cggctggtgg tcatcgtcgc
       61 cggttacacc gacgagatga agcgattcat agagtccaat cccgggctca aatcgcgctt
      121 cagtcgctat ttctattttg atcattacaa gccggacgac ctcatgaaga tcttcgatat
      181 ctcctgtacg aacgtccggt tcacggtgac cgatacggct cggaaaaaac tcgccgcaca
      241 gctcgaggcg ctctacgaaa agcgcgatcg atcgtttggg aacggccggt tggtcaggaa
      301 catattcgag aaaatcgtcg agcgccaggc gaaccgcatc gcgggaataa cgccgctgac
      361 cgatgagatc ctctgcacgc tcaccgagga agacattccc gaaaagatcg gccttgcgga
      421 ttgatcccgg catgcgtcag gccatcatcg cgtttgaagc attgcgtggc ggcacgtcgc
      481 acgcatcacg tcgcgcgtcg gtgcgcccgc ttttttggtt gacggttgtt gacgcatgcc
      541 cgtaattaac catttggtta aaatcaacgc tctacacagc acgcccctgc agcgtgagcg
      601 ctcgtacaga cgcaccccgc gttccgctcg atttgagaca tgcagctgtt gatgtgtggt
      661 atgcacaagg agacgagtat gcgcagtgta caaatcgtga tttcgatgat ccttctgttc
      721 cgcatgctgt cgcccgccgc tgcgcagcag gacgcagatt tcacgcccga gcggggcggc
      781 gagctcatca aggtggacat gggggacagc gccgccttca tgattggcgg aaacagggtg
      841 tcgctgggcg gcgccatcca catggtgctt gagcgcaata aggacacgct caccggcacc
      901 tacgatgccg ccatggtcga cacgatgtac aagaagttcc tgcgcaagta cgcgctcaat
      961 atcgacctcg agggaggata caagtaccag aagttccccg aatcgctgca gatgatgtcc
     1021 ggcgaagagc aacgctcgtg ggacgtttcg gccgcgctca gcaagtattt ttcgtctgga
     1081 acgtttgtgt cggccgggat catgcacacg tattccaaga ccgagtacat ccccattaac
     1141 ctcatgggca accagattac cttcggggac ccggaatata accggccggt gctcttcgtt
     1201 tcgatacagc aggagcttct gaaaaactcg ttcgggtata acgaacgtcg tcagaaagaa
     1261 atactgaaga acaccgcgaa gatgcagcgg gaggccctgg tctttcaact ggccggcctg
     1321 gtcgtgcaga ccgtgattga ctactggtcg gtcctggtcg gaaactcggg cctcgagaat
     1381 gcgcgactcc agcttcgcga gaccaaacga gtaagaaaca tcaccgcgca gaacgtgcgc
     1441 ctggggcttg gcgagagcta tgatctcaat tactacaaca cgctcgtcgc cacgtcggag
     1501 ggccgcgtgg cccaggccga acaggaatac cgggatacgc tgcggcgtct gctgcgcacg
     1561 ctcaacctgg aatcgaatct ggaactcagc ggaacggcgg tgttatcgaa caagcttccc
     1621 gttattaacg tagaaaaatc aatcgaaacg gcctattcga agcgggcgga ctacaagggg
     1681 gcgcaactca tgctcgacag cgctcgtcgc gagcttgagt tgtacaagaa cgaggacctg
     1741 ccgtcggtca tggccatcct caaggcgaac acgatggggc agcgcaagga acaatcggat
     1801 gcgtatgagg atgccgggaa gaacaaatac ccgtcatggg aggcgcggct caaggtcagc
     1861 tatccgctgg acaatacgga gcagaaggcc aacaagcgca acgctcgcta caagctcgaa
     1921 caggcgaaac tgcagttcga caagtaccag cgtctggtga aggacgaggt cgtcagcagc
     1981 gtcgagcgta tcaagaccga ttacgagctg taccagaaag cgcgcgaggc gcgggtgcag
     2041 gccgagctgc attatcggaa aatactggtg aacatgcggc gggggagggt gaccaatgcc
     2101 gtggtgaaga acgcgctgga cgccatgatc gacagccgcc agcgcgagct caacgcgctc
     2161 gtgcagtaca atatcaccct gttgcgctat gatctggcga ccaacttcat gttcgaccgg
     2221 tacaagatcg acgtgaacgc ctatattccc aattgacgat ttcggagcgg atgaaccatg
     2281 tttctgccgg ccacgcctga cgaggttgcg cggcgcggtt gggagcggct cgatgtggtc
     2341 atcgtgaccg gcgacgcgta cgtagacgcc ccgcagttcg gcgccgcggt catcggcagg
     2401 gtgctggagg acgcggggta tcgcgtcgga atcatcgcgc agcccgatat cgattcgccg
     2461 cgcgacatca cccgtttcgg cgaaccggcg ctcttctggg gcgtcaccgc gggcgctgtc
     2521 gattcaatca tatcaaacta cactgcgacc ggcaaacgcc gtaatcggga cgatctcacg
     2581 cctggcggcg tgaacgatcg gcgtcctgac cgcgcctcga tcgcctacgc caatctcata
     2641 cgccggtact tcaaaaacac cgcgccgatc gtcctgggcg gcgtcgaggc gagcctgcgg
     2701 cgagtcgccc actacgacgc ccgggacaat gcgctccgga ggtcgatcct cttcgatgcg
     2761 aaggccgatt acctcgccta tggcatggcg gaacggacgg tcctgcacct cgcatccgcc
     2821 atcaagaatg gttgcgatac gcgggatata tcggggttgt gccatatcgc gaaacaggtc
     2881 ccggaaggtt acattgagct tccaggattt gaatcgtgcc tctcggacaa ggacgccttc
     2941 attgcgatgc atcgccttct cgctgaaaac atggattccc ggtcggcgcg tggacttgtc
     3001 cagcggcatg gtgaccgcta tctcgtccac aatccgccgc caatcgatgt atccccagag
     3061 gagctggacg cgatacacga gctcggatac gagcgcgagg cgcatccgct ctgccgtgcg
     3121 cggggagagg tccgcgcttt ggacaccgtg cgcttctcca tcacgacgca ccggggctgc
     3181 ttcgggggat gcagtttctg cgcaattgcg atccatcagg ggcgacggat cgtttcacgc
     3241 agcatcgaat cgatcgtgcg cgaggctcga tcgttcctgg ggcatccttc gttcgcggga
     3301 atcatcagcg atctcggtgg gccaacggcg aacatgtatg gcatgtcgtg cgcgaatgac
     3361 gacgctccgg tgtgcgcgga acggcagtgc gcggggatca gtgtgtgcag aacgctcgtc
     3421 tgcacgcacg agcgctcaat agcgcttcta cgcgccgtgc ggtcggtgcc gggaatacgc
     3481 aaggtctttg tcgcatcggg ggtccgtcac gatctcgtgc tcgccgatca tgagttcggc
     3541 cgcgtatatc ttgaggagct cgtggagcat catgtttccg gccagctgaa ggtcgcgccc
     3601 gagcattccg aacccgcggt gcttcgtctc atgggcaagc ctgatttcga ctcgctgcgt
     3661 gagtttaaac agctgtttga tgatatcgtt cgcgcgaaac gggttccgca ctacctgacc
     3721 tattacttca tcgcagcgca ccccggatcg actgacgccc acatgcgcgc tttgcacggg
     3781 aagatctcac gcgaattgcg catcacgccg gaacaggtgc agatcttcac tccgtcgccc
     3841 tccactgcgg cgacgttgat gtattacacc ggcgtaaacc catccgacgg agccgctgtg
     3901 ttcgttgaac gggaccggag ccgtcgagag cggcaaaaac ggatcattac gggttgatgc
     3961 gatcgtcgat ggtcgccagt cacgtcctgt cgggagcgtc gtccggcacg atcaattggg
     4021 cgatcgtcgc gccccagttc cctgcctgat cgctgaacgt ggcgacgcgg ggatcgtttt
     4081 cgagaaatgc gataacaatt gattttatga ccgactgtcc cttgccgtgc acgatgcgga
     4141 cgctacgata ccgtttggcg atcgcgtgtt cgatgaaatc gccaaggagc gccttgacgt
     4201 cgcgggggtg gaaggcgtgg aggtcgattt cgtcggtgag ttcaaccggg acgaaatcgt
     4261 catccatgaa taatcgctac cagatgaaga tgaggggatc gaccagctgc ccatgccgct
     4321 ttacgacgaa gtggaggtgc gacccggtcg accggccggt cgaaccgacc tctgcgatga
     4381 tatcgttctt ggtgaccatg tcgccctgcg caacgcggat caccgagcaa tggccgtaca
     4441 tggtctcgta tccgttccgg tggaggacga agacggaaag cccatagccc tcgttccagc
     4501 cgacccggct caccatgccg tcggcaagcg gacggatcgg cgtcccggtt ggggcgcgga
     4561 tatcgactcc attgtgaaag gtggcctctt tgtagatggg atcattgcgc acaccgaaaa
     4621 acgaggtgaa ctgaccgcgc accggcaccg cgaatgccat gcggatatcg tagaggtcct
     4681 gcagcgcctc acccacgatg cgcggtttcg cattcttgat gaatacgaac cgaatatcgt
     4741 cgagcgacag gaggtcgaat acgccctggc ggaaatctcc ctttacgcgc atggaaagcg
     4801 ggttcccgac gcgggtgagc atgcgcacgg cgtcggcggc atcgtcgatg ggaaaaagca
     4861 cgccgttttc gcttgggatg acgagttcga gtccctcagt tgccgcgagc gacggcaaat
     4921 gtggatttgc gccaataatg gtttcaagag aaatgttatg tcgatgggcg atgtcccagt
     4981 aggattctcc cgcctggatg cggtggatcg taacggttgt tccgaaattg taccgatagg
     5041 tcttgacgag tttcagatat gcatccgctg atgtgaacac cggaaatccg tcacgtaaac
     5101 ggtaatcctg cggattgacg acgatgcgtt ccttggtcgc cctaacgaaa aggttgccgc
     5161 cgacgaagat cgttatgccg gcaagcagga ggatgaccag atagcgcaca gccttcgaag
     5221 gctcgctcga agtggtctgc agggccttcg gtaccggccg aaatcgtttc atgtccagca
     5281 tcagcttttt ctccccagcg cctcagattt cttggccgcc gcctcgatcg catccatcac
     5341 gatccccgag aacccggcgc gctccaaaac gtgaacggcg tcgatcgtgg tgccgccggg
     5401 cgaggtgacc tgcccgcgga gttctatggg atcggctccg gcctcaagga cgagtctcgc
     5461 ggcgcctgct gcggtctggg ccgccaattc cagcgccgtt ttgcgcggta tgcccatttt
     5521 cacggcgccg tccgtcatgg cctgaataag catgaaaacg tatgcaggcc cgctgcccga
     5581 aaccgccgtt accgcatcca tcatccgttc gggaagcacc gcgacccgtc caagcaagcc
     5641 gaatatgtcc gtagcctcgc gaatctcatc gctgccgacg gtatcgtgcg gcgaaatcac
     5701 aatcatcccc tcgccgatct ttgccggcgt gtttggcatc acgcgaagta ttttcttgtc
     5761 cggcccaata acggccgtaa tggattcgat ggatacgccc gccgcgatcg aaatgatcgt
     5821 ctggttgcgg atacgatcgc gcgcttctgt aagaactttt tttacaccgt ctggcttgac
     5881 tgcgattata attatgtcgc ttttttctgt caagtcatta atggtgctca ctccctggat
     5941 tccgtatagc tttacggccg cggcctgttt ttcagcatca atatcgtagc aataaatcat
     6001 ctccgtggat tgggcgctcg ccagccggga gaggattgcg ccgcccatat tgccgacgcc
     6061 aatacatgcg attcgcttat tgacggtttg ttgcatcgta atccctctgc ccgaaaatgg
     6121 ccgtaccgat ccgtatcatg gtcgcgcctt cttcgatggc gacatgatag tcggacgtca
     6181 tacccatgga taattcgttt atggtcattc cgtatgaact gttaatatca tcaagcagat
     6241 tttttagcaa tctgaacgat tttcttattg tatcgatatt atcggtaaaa ggaccgattg
     6301 tcatcaagcc gcgcacgtcg atgtttttca ggtcc
//
LOCUS       DAORHF010000212         6325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12327_length_6325_cov_5.915789, whole genome shotgun sequence.
ACCESSION   DAORHF010000212 DAORHF010000000
VERSION     DAORHF010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12327_length_6325_cov_5.915789"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="PKJ16_14620"
     CDS             complement(<1..929)
                     /locus_tag="PKJ16_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNV48276.1"
                     /translation="MLLGLDIGGTHTDVVAIGKDGIAASAKVVTNHDDLLASVTGALE
                     RVFSAIDVAAVDRINLSTTLSTNAIVERTTEEVGVIVSAGPGVDPQAHAVGNRYYAIA
                     GSIDHRGTEVLPPAEGEIQKAVRHCLKNGVKVFAAVSKFSTRNPAHENRIRDIIQGNA
                     DFTTVGHELSGALNFPRRVSTAYFNSAVWRIFNRFADAIESGVVAHGIAPAINILKAD
                     GGTMPLAVARRIPVESILSGPAASVMGIVALCDIVEDAVVLDIGGTTTDIAIFADGAP
                     LVEDGIELDERATLVRALKTRSIGIGGDSILAV"
     gene            1232..>3165
                     /locus_tag="PKJ16_14625"
     CDS             1232..>3165
                     /locus_tag="PKJ16_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007291473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48277.1"
                     /translation="MSRKRILLAEDESIISLDIASILKELGYEMAGIVATGEAAIAEA
                     ESSRPDLVLMDIVLKGGMDGIAAAEAIQTRLGIPVIYLTGNADMGTVSRARQTNPYGY
                     ILKPVSAQDVLTTIDTALHRHELERAVREKNAELAALNEELGQTLEELESTNEQLRVS
                     NESLEREAAERASVELERDERVREVELFFRSGLDLFCIADTDGYFRRLNPEWERTLGY
                     SIDDLEGKRFLDLVHPDDVEATMNAVGKLAGQREVLNFTNRYRCKDGSYRWIEWRALP
                     AGTLIYAAARDITEQRAAMESLAESEARFRTLFDTTTLGIVYQAADGRIELANQAALD
                     ALGLTLDQVQGRTSFDPRWRSIYEDGADCPGDEHPAMVALRTGETVRGRVMGVYRPSD
                     DSYRWLLIDAVPDFRPGEERPYRVFATFIDVTERRSIADALRESEGRYRLLFENMTSG
                     FALHEMLYDDAGEPVDYRFLEINPAFERLTGLRAESLIGKTVREALPGTEEYWIRTYG
                     EVAMTGEPRSFQNYHQELDRYYDVIAFCPQRGMFAVVFSDVTEAQRTHRILQASLREK
                     ETLLKEIHHRVKNNFQLVTSLLSLQSMSVNDEHLEREFSEAQGRVRSMALVHEKLYQS
                     ADLGSIDFAEYAREMSQSLAR"
     assembly_gap    3166..3265
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3266..3687
                     /locus_tag="PKJ16_14630"
     CDS             <3266..3687
                     /locus_tag="PKJ16_14630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV48278.1"
                     /translation="GSIDFAEYAREMSQSLARSFAGGRRAPNIAVNAVEVRLGVDKAI
                     PCGLILNELVTNSCKHAFPESWRGDPAIAVDLSEEGGAVTLRVSDNGVGIADGVALEQ
                     PATFGLSLVSLLARQLRGTIRVARDGGTAVTVEFPGE"
     gene            3711..4070
                     /locus_tag="PKJ16_14635"
     CDS             3711..4070
                     /locus_tag="PKJ16_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HNV48279.1"
                     /translation="MGSDQSFVDFVLDNIEQAGELSARKMFGEYAIYCDGKVVALVCD
                     NRLFVKPTPGGRAHIGDPAEAPAYPGARPSFLVEDRIEDREWLSELIRITARELPAPT
                     PKKPKKTKKTGESRGAR"
     gene            4111..5823
                     /locus_tag="PKJ16_14640"
     CDS             4111..5823
                     /locus_tag="PKJ16_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNV48280.1"
                     /translation="MSVLDLRTVFLTGVIINAICTLVAVLLWRSSRDRFDGTGLWAAD
                     FAAQTAAFFLIGLRGVLPDVVSISIGNALSVLGALVGLLGLGRFWGKPVFTGVQWGIF
                     AVYLALHNYYTYFEPSLAARNLLFSASLLALCGQCAWLLLYRVSSAVRSMSLIAGIAM
                     TGLTVVSAVRIVIEVFVPIQTQDYMHSGTANAMYIVLYLALIVFLAYALALMVNRRLL
                     ADLGSQEQKFSKAFHSSPDAVILTRLADGSIYEVNDTFTRMTGFTAAEVIGKTTVDLG
                     LWADANDREIFIGRLREHGSVFGLEFTFRIKSGELLVGQLFSDVLVINEVPTIVASIS
                     DITERKRAEERLIVAMQESDMLRERAERLGAEKEIILREVHHRIKNNLSTVHGLLSLQ
                     ANRLSDPQAAKSLRDAVGRVRSMGLLYDKLYRAESIGRMSMKTFLERLVAEIAELYPA
                     PVPVAVSADIEEATLDAKILSPLGIMMNELVTNAMKYAFTGRASGRLDVRSRIEDRGI
                     TIIVEDNGIGLPDGIDIDRSTGFGMQLVGLLAKQIGATIRVERGEGTRFVIEFTAQSP
                     ERTV"
     gene            5944..>6325
                     /locus_tag="PKJ16_14645"
     CDS             5944..>6325
                     /locus_tag="PKJ16_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48281.1"
                     /translation="MKNIISIRDSSAIRTNFRHAIRKTRRVFGAFALLIFQALALAAC
                     ICAAAVPVQAQYDPTDTAKRPAESVADPGKKEDKGAGVTAGVDYWTLYFWRGDLLYGD
                     NGDVFFPFVKWAIFDTGFTLTWMGE"
BASE COUNT         1111 a         1872 c         2092 g         1150 t
ORIGIN      
        1 ctcacggcga ggatcgagtc gccgccgatg ccgatggagc gcgttttaag cgcgcgcacc
       61 agcgtcgccc gttcgtcgag ctcgatgccg tcctcgacga ggggcgcgcc gtcggcgaag
      121 atcgcgatgt cggtggtggt gccgccgatg tccaggacga cggcgtcctc gacgatgtcg
      181 cacaacgcca cgatgcccat cacgctcgcg gccgggccgg agaggatcga ttccaccggg
      241 atgcgccgcg cgaccgcaag cggcatggtg ccgccgtcgg ccttcaggat gttgatggcg
      301 ggggcgatgc cgtgggcgac cacgccggat tcgatcgcgt cggcgaagcg gttgaagatg
      361 cgccagacgg ccgagttgaa gtaggccgtg ctcacccggc gcgggaagtt gagcgcgccc
      421 gagagctcat ggcccaccgt ggtgaaatcg gcgtttccct ggatgatgtc gcggatgcgg
      481 ttttcgtgcg cgggattgcg cgtggagaac ttggatacgg cggcgaacac cttcacgccg
      541 ttcttgaggc agtggcgcac ggccttctgg atctcgccct cggcgggcgg cagcacctcg
      601 gtcccgcgat ggtcgatgga gcccgcgatg gcgtaatagc ggttcccgac ggcgtgcgcc
      661 tgcggatcga cgccgggacc ggccgagacg atgacgccca cctcctcggt tgtgcgctcc
      721 acgatcgcgt tggtcgagag cgtggtggag aggttgatgc ggtccacggc cgcgacgtcg
      781 attgccgaga acacgcgctc gagcgcgccg gtgacggagg cgagcagatc gtcgtggttg
      841 gtcacgacct tcgccgaggc ggcgatcccg tccttcccga tggccacaac gtccgtgtgt
      901 gtgccgccga tgtccaggcc aagaagcatg agccacctcc cggagtgtat gatgacgccc
      961 gtgttgacgc gggtccgcag cctgaggacc gagtcgtgcc gcgagcatac tatcgacgcg
     1021 cttcctgtca agacgataaa atgaatccat cgcgcgggat catcgggcca cggcaatgct
     1081 tgtttcgtgg gaacgggagc catgccaggt aaacaaaaag taaaatatgg cccagtgggg
     1141 cgagggtcga ttcgcagacg ctttttttat tgatttgacg ggggcgttcg catatcatac
     1201 caccgaggtc gtgaaagagg agcgttcgcg aatgagccga aagaggatcc tcctggcgga
     1261 ggatgaatcg ataatatcgc tcgacatcgc ctccattctc aaggagctcg gctacgagat
     1321 ggcgggcatt gtcgcgaccg gagaggcggc gatcgctgaa gccgagtcgt cccgaccgga
     1381 cctggtgctc atggacatcg tactcaaggg cggcatggat ggcatcgcgg ctgcggaggc
     1441 gatccagacc cgcctgggga tccccgtgat ctatctgacc ggcaatgcgg acatgggcac
     1501 ggtgtcgcgc gcgcgccaaa cgaacccgta tggctatatc ctcaagcccg tgagcgcgca
     1561 ggacgtgctc accaccatcg atacggcgct ccaccggcat gagctcgagc gtgcggtacg
     1621 ggaaaagaac gccgagctcg cggcgctcaa cgaagagctg ggccagacgc tggaggagct
     1681 cgaatcgacg aacgagcagc tgcgcgtctc gaatgaaagc ctggagcgcg aggccgcgga
     1741 gcgggcgagc gtcgagctcg aacgcgacga gcgggtgcgg gaggtggaat tgttcttccg
     1801 cagcggtctc gatcttttct gcatcgcgga caccgacggg tattttcgcc gcctcaaccc
     1861 cgagtgggag cgaacgctgg gatattccat cgacgacctc gagggaaagc gttttctgga
     1921 cctggtgcac cccgatgatg tcgaggccac gatgaacgcc gtcgggaagc ttgccgggca
     1981 acgtgaggtg ctcaacttta cgaaccggta tcgctgcaag gacgggtcgt accgatggat
     2041 cgaatggcgc gcgcttcccg cagggacgct catctatgcc gctgcgcggg acatcaccga
     2101 gcagcgcgcc gccatggaat cgctcgcgga gagcgaggcg cgcttccgca ccctgtttga
     2161 cacgaccaca ctggggatcg tgtaccaggc ggccgacggc cggatcgaac tggcgaacca
     2221 ggccgcgctt gacgccttgg ggctcacgct cgaccaggtg cagggccgca cctcgttcga
     2281 tccgcgctgg cgctcgatct atgaggacgg cgcggactgc cccggagacg agcatcctgc
     2341 catggtggcc ctgcgcaccg gcgaaacggt gcgcggcagg gtgatgggcg tgtaccggcc
     2401 gagcgatgat tcgtaccgct ggctgctgat cgacgcggtg ccggatttcc gtccgggcga
     2461 agagcgtccg tatcgggtct tcgcgacctt catcgatgtc accgaacggc gaagcatcgc
     2521 ggacgcgtta cgcgaaagcg aggggcggta ccggctgctc ttcgagaata tgaccagcgg
     2581 gttcgcgctc cacgagatgc tgtacgatga tgccggcgaa ccggtcgatt accggtttct
     2641 cgagatcaac cccgccttcg agcggcttac gggactgcgc gccgaatcgc tcattggtaa
     2701 aacggtgcgg gaggcgctcc cggggaccga ggagtactgg attcggacct acggtgaggt
     2761 cgcgatgacc ggggagcccc ggtcctttca gaactatcac caggagcttg atcgatatta
     2821 cgatgttatc gccttctgtc cgcagcgcgg gatgttcgcg gtggtgttct ccgacgtgac
     2881 cgaggcccag cggacgcacc ggatactcca ggcctcgctt cgcgagaagg agacgctgct
     2941 caaggagatc catcatcgcg tgaagaacaa cttccagctc gtgaccagcc tgctgtcgct
     3001 gcagtcgatg agcgtgaacg acgagcacct ggagcgcgag ttctccgagg cgcaggggcg
     3061 cgtgcgctcg atggcgctgg tgcacgagaa gctgtaccag tccgccgacc tggggagcat
     3121 cgatttcgcc gagtatgcgc gcgagatgtc gcaatcgctc gcgcgnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnntgggg agcatcgatt tcgccgagta tgcgcgcgag
     3301 atgtcgcaat cgctcgcgcg gtccttcgcc ggcggacggc gcgcgcccaa catcgcggtg
     3361 aacgccgtcg aggtccggct cggggtggac aaggcgatcc cctgcgggct catcctcaac
     3421 gagcttgtca ccaacagctg caagcacgcc ttcccggaat cctggcgggg cgatccggcg
     3481 atcgcggtcg atctttcgga ggagggcggg gcggtcacgc ttcgtgtgtc ggacaacggc
     3541 gtcgggattg ccgacggcgt ggccctcgag cagcccgcga ccttcgggct ctcgctggtg
     3601 tcgctcctcg cgcggcagct tcgcgggaca attcgcgttg cgcgcgacgg cgggaccgcg
     3661 gtcacggtcg agtttccggg tgagtgagac gtaatatcaa ggagatggcc gtgggatccg
     3721 atcagagctt tgtcgatttc gttctggaca acatcgaaca ggccggggag ctgtccgccc
     3781 gtaaaatgtt cggcgagtac gcgatctatt gcgacgggaa ggtcgtcgcg ctcgtatgcg
     3841 acaaccggct cttcgtgaaa cccacgccgg gcgggcgcgc gcatatcggt gatccggcgg
     3901 aggccccggc ctatccgggc gccaggccct cgtttctcgt cgaggaccgg atcgaggacc
     3961 gggagtggtt gagcgagctc atccgcataa ccgcgcgcga gctccccgcg cccacaccga
     4021 aaaaaccgaa gaagacgaag aaaacaggcg aatcgcgggg ggcccggtga gcgcgatgtt
     4081 cgattttggt ttgattccgg cggcgccgca atgagcgttc tcgacctgcg tacggtattc
     4141 ctgaccggcg ttatcatcaa cgcgatctgc acgctggtgg ccgtgctcct gtggcgcagc
     4201 agccgcgacc ggttcgacgg gacgggcctg tgggccgccg acttcgccgc gcaaacagcg
     4261 gcctttttcc tgattgggct ccgtggcgtc ctgcccgatg tcgtctcgat ttcgatcggg
     4321 aacgcgctct ctgtcctcgg agcgctggtg gggctcctcg gattgggacg cttttggggc
     4381 aagccggttt ttaccggcgt tcagtggggc atcttcgcgg tctatcttgc attgcataat
     4441 tactatacgt atttcgagcc gagcctcgcg gcgcgaaacc tgctcttttc agcctcgctt
     4501 cttgcgctgt gcgggcagtg cgcctggctt ctgttgtacc gggtctcgtc ggccgtgcgc
     4561 tcgatgtcgt tgatcgcggg catcgcgatg acgggtttaa ccgtggtgag cgccgttcga
     4621 atcgtcatcg aggtttttgt accgatacag acgcaggatt acatgcactc tggcaccgcc
     4681 aatgccatgt acattgtcct ctacctggcg ctcatcgtat tccttgccta cgctctggcg
     4741 ctcatggtga atcgccgcct gctcgccgat cttggctcgc aggagcagaa gttctccaag
     4801 gcgtttcact cctcgccgga cgcggtgatt cttacgcgcc ttgccgacgg ctccatctac
     4861 gaggtgaacg acacctttac gcgcatgacc ggatttaccg ccgccgaggt gatcggaaaa
     4921 acgaccgtgg atctcggcct ctgggcggat gcgaacgacc gcgagatctt catcggccgc
     4981 cttcgcgagc acgggtcggt tttcggtctc gagtttacgt tccggataaa atcgggcgag
     5041 ctcctcgtgg gacagctttt ttcagatgtc ctcgtcatca acgaggtgcc cacgatcgtc
     5101 gcgagcatca gcgatatcac ggagcgcaag cgcgcggagg agcgcctcat cgtggcgatg
     5161 caggaatcgg acatgcttcg ggagcgggcg gagcgactgg gcgccgaaaa ggagatcatc
     5221 ctgcgcgagg tgcaccatcg catcaagaac aacctgagca cggtgcacgg cctattgtcg
     5281 ctgcaggcca acaggctgtc cgatccgcag gccgcgaaat cgcttcgcga tgcggtgggg
     5341 cgcgtgcgca gcatggggct tttgtacgac aagctgtacc gcgcggaatc catcgggcgc
     5401 atgtcaatga agacgtttct cgagcggctc gtcgccgaga tcgccgaact ctatcccgcg
     5461 ccggtgcccg tcgccgtgtc cgccgacatc gaagaggcga cgctggacgc gaaaatcctc
     5521 tcgcccctgg gcatcatgat gaacgagctc gtgaccaatg ccatgaagta cgcctttacc
     5581 ggtcgcgcgt ccgggcgtct tgacgtgcgc tcccgcatcg aggatcgagg catcacgatt
     5641 attgtcgagg acaacggcat cggcctcccg gacgggatcg acatcgatcg ttcaaccggt
     5701 ttcggaatgc agctggtggg cttgctcgcc aagcagatcg gcgcgaccat ccgcgtcgag
     5761 cggggcgagg ggacgcgatt cgtcatcgag tttaccgcgc agtcgcccga acggacggtg
     5821 tgacgttcgc cgaacccccg cggcgatttt cgcgatatcg accgggggcg atggcgctta
     5881 aagacatcaa tcaaacggtg cgcgaacggc aggccggtcc tgccggcgcg tgaggaggga
     5941 cgcatgaaga acatcatatc gatacgcgat tcgtccgcga tccgcacgaa cttccggcat
     6001 gccatcagaa aaacacgcag ggtgttcgga gcgttcgcgt tactgatctt tcaggcgctc
     6061 gcgctcgcag cgtgtatatg cgcggcggcc gttccggtcc aggcgcagta tgatccgacc
     6121 gacaccgcga aacggcccgc ggagtcggtc gcggaccccg gaaagaaaga ggacaaaggc
     6181 gcgggcgtga cggctggagt ggactactgg acgctctact tctggcgtgg agacctgctc
     6241 tatggcgaca acggcgatgt gttctttccc ttcgtgaagt gggcgatctt cgacaccggg
     6301 ttcacgctca cctggatggg cgagt
//
LOCUS       DAORHF010000213         6293 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12426_length_6293_cov_4.550818, whole genome shotgun sequence.
ACCESSION   DAORHF010000213 DAORHF010000000
VERSION     DAORHF010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12426_length_6293_cov_4.550818"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1100
                     /locus_tag="PKJ16_14650"
     CDS             96..1100
                     /locus_tag="PKJ16_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48282.1"
                     /translation="MKIISILLFLAVASPLAAAYEKYVHLQYIERMEETPLPFLKAKK
                     AESAGQALNGAEYAVSIKEYKMACEDFASAIAAEVDPYKRAFIYTRLGETYLELKEYQ
                     KAIACLANAEGILSKNRSDFEQLHAQLPRVYAATSRCHLRWGKIASAAFYLWAANRLF
                     DELGQGDAAKRPFLDDAQKKLVKSPLYGFISGRAASCDDRGTVKTLYASMGEDDGGFA
                     AFRKKARMTTHVYLSPRPQGPMKAGANGARFAVYTKGGRTAIVEFRDPEAEDFNKRIC
                     YRDGKPVSSVYESHTYHSTDVKLRYGPRGDLLWVLVVSYPTQVNHHGFIPTVNFYSFE
                     "
     gene            complement(1110..2150)
                     /locus_tag="PKJ16_14655"
     CDS             complement(1110..2150)
                     /locus_tag="PKJ16_14655"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HNV48283.1"
                     /translation="MEHAHRFALACAIIALSCSAATAEPVFSNSPAITKRYNDATIVD
                     IAFSPDRAYFASVNKDNSVKMWNPVTGALMKTMTGHAATLRYVSVSPDGKYIASASIA
                     NEPVKIWDAATGTLVRDIMAQDGCMGIAFSPAGGLLATCGGLINDKNMAGAILWDTAT
                     GARKAVLMGEKSDLSHPTSLSFSRDGKFLAVGLANKKPGIRVFDTATGKLVRAIAHSL
                     DINAVDFSPDGSMIAGGGGGSGKSFRVYLWNAATGKLIRTHEGHTAFITSVRFSPDGT
                     LLASSSFATDTRFRLWNPATGALVQSMDKTRIRTNAVHFSPDGSRLAAVLVTYGDLGN
                     PPVLEVYEAGGK"
     gene            2568..3776
                     /locus_tag="PKJ16_14660"
     CDS             2568..3776
                     /locus_tag="PKJ16_14660"
                     /inference="COORDINATES: protein motif:HMM:NF018584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HNV48284.1"
                     /translation="MGEISHEQKLGLAMAAMYNEVNGLEYDSLAGCEPGFLNKRAAKG
                     MLADSWGIKGHDELVGALKWLYEEGHRADHAAEGHEPNTILAWDLCRLINLVRMGTRV
                     EYIDEKEGWDWVMKSSQLMQSAFKSWNEVAESFISGRSYWLQKQEMEDDYDDEMHEAA
                     ETLLDAGNDESIWNQLRFPGSEAVVPGYEDESKYFTEHGEAIQAGAAAIATDRDTLND
                     AISGGDASAVKTCLASAPDLKKKSEDGTTPLSLAIYYGNDEIAKLLLDAGADPNGKNE
                     YDVPLLCYAIDQENPKMVKRLLDAGANPNAKNEDGWPALMMALYREDADTIRAVIAAK
                     PDLNAADPDGVTILHYAISNENADQVKALVAAGADVNKKNGDGYMPLEIAEESEMEEI
                     VKVLKKAGAK"
     gene            complement(3843..4853)
                     /locus_tag="PKJ16_14665"
     CDS             complement(3843..4853)
                     /locus_tag="PKJ16_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48285.1"
                     /translation="MDKRTKLVMKTICPSCGAAKATAAQYAYVYCDYCGGYMDFDMQS
                     ALTFTGQMYDEKYSADCEKFHKARERAKAAGDKKAFIKIGRGLYGLQMEKSPHAWSVR
                     IGDPPYRATLIENQSQVDALMAFDADLAHIYDVKLVEAYNGLKSAMTPSGLKYSSASV
                     WNYFRVNYEGLVETMKKRSTWDVPIVHPDETDPRIMIKGWASAFLQGWMPYLFDAAKQ
                     QIIEYAGLESEYLKAKLPDASQRTCGKCGGPITAPPGSRQLVCEGCGYTIDVESAELP
                     CPGCSAVLSIPVTADALSCPHCGTRYRDSGSGGHTVTYNGKTMDILDAMIEDIQRAKP
                     PK"
     gene            5071..5313
                     /locus_tag="PKJ16_14670"
     CDS             5071..5313
                     /locus_tag="PKJ16_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HNV48286.1"
                     /translation="MWSFIVFLVIGIIAGWLAGLIFKGRGFGLIWDMVIGVAGSFIGG
                     FVFKLIGVGAYGFLGSVFTAVVGALLLLFIINWIKK"
     gene            complement(5802..>6293)
                     /locus_tag="PKJ16_14675"
     CDS             complement(5802..>6293)
                     /locus_tag="PKJ16_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48287.1"
                     /translation="YQDNDKPHSADIKGSECMTVTSGIATGEGASRENVTAIKRPLEG
                     QTLEALIKEVKQLHQRAIIVIEPMYKDVPESIEEVKKDIVKEITITEKIDTDGQKTVV
                     LVLKTEKQIRDHYNKLEQVYLEDGSVIVGAIISQSKTNARIHTTNGIISVPTDSINRV
                     IMR"
BASE COUNT         1231 a         1766 c         1962 g         1334 t
ORIGIN      
        1 ggctggatta agaatgttgc ttaactgcgt gtccgcaaaa tcggggcaag cccaaggtgc
       61 ccgaagggcg gagggggtcg cgttgaggta aaatcatgaa aattatttcg atccttttat
      121 tcctcgccgt cgcatcgccc cttgcggccg cctatgagaa gtacgtccac ctgcagtaca
      181 tcgaacgcat ggaggagacg ccgctgccct tcctgaaggc gaaaaaggcc gagtccgcgg
      241 gccaggcgct taacggggcc gagtatgctg tgtccataaa ggagtacaag atggcatgcg
      301 aggatttcgc gtccgccatc gccgccgagg ttgatcccta caagcgcgcg ttcatctaca
      361 cccgccttgg cgagacctat ctcgagctga aggaatatca aaaggcgatc gcgtgcctcg
      421 ccaacgccga gggcatcctc tcgaagaacc ggtccgattt cgagcagctg cacgcgcagc
      481 tgccgcgcgt gtatgccgcg acgagccgat gccaccttcg ctgggggaag atcgcgagcg
      541 ccgcgtttta tctctgggcc gcgaaccggc tcttcgacga gctgggacag ggggacgcgg
      601 ccaagcggcc cttccttgac gacgcgcaga agaagctcgt gaaatcgccg ctctacggtt
      661 tcatcagcgg gcgcgcggcg tcgtgcgacg acagggggac cgtgaagacg ctgtatgcgt
      721 cgatgggcga ggatgatggc ggtttcgccg cgttcaggaa gaaggcccgg atgaccacgc
      781 acgtgtatct ctctccgcgg ccgcaggggc cgatgaaggc cggagcgaac ggcgcgcgat
      841 tcgccgtcta tacgaagggc ggacggaccg ccatcgtcga attccgcgat cccgaggccg
      901 aggatttcaa caagcggatc tgctatcgcg acgggaaacc cgtttcgtcg gtatacgaat
      961 cacatacgta ccattcgacc gacgtcaagc tgcgctatgg tccgcggggc gacttgctct
     1021 gggtgctcgt cgttagctat cccacgcagg tgaaccatca cgggttcatt cccacggtga
     1081 acttctactc gttcgagtag tcccgcccct cactttccgc ccgcttcata cacctcgagc
     1141 accggggggt tccccaggtc gccgtaagtg acgagcaccg ccgcgaggcg gcttccgtcc
     1201 ggcgagaaat gcaccgcgtt cgtgcggata cgcgtcttgt ccatggattg cacgagcgcc
     1261 ccggtcgccg ggttccacag gcggaagcgc gtatcggtcg cgaaactcga acttgcgagc
     1321 agcgtgccat ccggactgaa ccggaccgag gtgataaacg cggtgtggcc ctcgtgcgtg
     1381 cggatgagtt tgccggtggc ggcgttccag agatagacgc ggaacgactt cccgctcccc
     1441 ccgccgcccc cggcgatcat cgaaccgtca ggagagaagt ccaccgcgtt gatgtcgaga
     1501 ctgtgcgcga tggcgcgaac gagcttcccg gtcgcggtgt cgaagacgcg gatgcccggt
     1561 ttcttgttcg cgaggcccac cgccagaaat ttcccgtcgc ggctgaagga caacgaggtt
     1621 ggatgcgaca ggtccgattt ctcccccatg aggacggcct tccgcgcgcc cgttgccgtg
     1681 tcccagagga tggccccggc catgttctta tcgttgatga gccccccgca ggtggcgagc
     1741 agcccgccgg ccggcgagaa ggcgatcccc atgcagccgt cctgcgccat gatgtcgcgc
     1801 accagcgtgc cggtggcggc gtcccatatc ttcaccggct cgttggcgat ggaggcgctg
     1861 gcgatatatt ttccgtcggg gctcacggaa acatagcgca gggtcgccgc gtgcccggtc
     1921 atggttttca tgagcgcgcc ggtcaccgga ttccacatct tcacgctgtt gtccttgttc
     1981 acgctggcga aataggcccg gtccgggctg aaggcgatat cgacgatggt cgcgtcgttg
     2041 tagcgcttgg tgatcgccgg tgaattcgaa aacaccggct ccgcggtggc ggccgagcac
     2101 gagagcgcga tgatggcgca ggcgagagca aatcgatgcg cgtgttccat gggttcctcc
     2161 tggtgcatgg cgatgtcgta ttcgaagcag ggtaggtgcg gtttgcgtat tatgcaagat
     2221 aaaaaaccgt tcggcgccgc cccgcccgcg gccaatattc gattgacggt atatcgcgca
     2281 ccgggtcgag gaccaaattc gcgcaaagat cgaccggagt gttgtacaca ttcacgagga
     2341 gccggaaaaa gtactgaatc aacgcgcgaa acggacgaac ggctgatggc ggatcaagca
     2401 acgatgcgca ggttccgctg aggatgcgct cttcgtaaaa tcgacgccta cccgcatact
     2461 gtactattca gaatgaaaag agcttgctat cctgtgcgca tcgagaaaat cctatccgaa
     2521 cttgtaacgg attcgaaata tatcaacaat gagcggagga attactgatg ggggaaataa
     2581 gccatgaaca gaagttgggg ctcgccatgg cggcgatgta caacgaggta aacggcctcg
     2641 agtatgattc gctcgccggg tgcgagccgg gcttcctgaa caaacgcgcg gcgaagggca
     2701 tgctggccga cagctggggt atcaagggac acgatgaact cgtcggtgcg ctgaaatggc
     2761 tctacgagga gggccatcgt gcggatcacg cggcggaggg tcatgagccg aatacgatcc
     2821 tcgcctggga tctgtgccgt ctcatcaacc ttgtgcgcat gggcacccgg gtggagtaca
     2881 tcgacgagaa agaggggtgg gactgggtta tgaagagttc tcagctgatg cagtcagcct
     2941 ttaaatcctg gaatgaggtc gccgagagct tcatttccgg ccgctcctac tggcttcaga
     3001 agcaggaaat ggaggatgat tacgacgacg agatgcacga ggcggccgag accctgctgg
     3061 acgcggggaa tgacgaaagc atctggaacc agctgcggtt ccccggcagt gaggcggttg
     3121 ttccgggcta cgaggacgag agcaagtatt ttaccgagca tggcgaggca atccaggcgg
     3181 gagcggcggc gatcgcgacc gaccgcgaca cgttgaacga tgcaatatcc ggcggggatg
     3241 cgtcggctgt gaagacgtgc ctcgcgtcag cgccggacct aaagaagaag agcgaggatg
     3301 gaactacgcc gctgagtctt gccatctact acggcaatga cgagatcgcg aagctgctac
     3361 tcgacgcggg cgcagatccc aacgggaaga atgagtacga tgtgccgctc ctgtgttatg
     3421 cgatcgatca ggaaaatccg aaaatggtga agcggctgct cgatgccggc gcgaatccga
     3481 acgcgaagaa cgaggacggc tggccggctc tcatgatggc gctgtatcga gaggacgcgg
     3541 acaccatccg ggcagtcatc gccgcgaaac ccgatctgaa cgccgccgat cccgatggcg
     3601 tgacgattct gcattacgcg atcagcaacg agaatgccga ccaggtgaag gcgctcgtgg
     3661 ccgccggcgc cgacgtgaac aagaagaatg gcgacggcta catgcccctt gagattgccg
     3721 aggagagcga gatggaggag atcgtgaagg tattgaagaa ggccggtgcg aaatagggtc
     3781 ggccgatctc ccgacccctt cgtctgctgc acatcggccc gggcgtcaat ccgggccgat
     3841 ttttatttcg gcggcttcgc gcgctgaatg tcctcgatca tcgcgtcgag gatgtccatg
     3901 gtcttgccgt tgtacgtcac cgtgtggccg cccgagccag agtcccggta acgcgtcccg
     3961 caatgcggac aggagagcgc gtcggcggtc accgggatcg acaggacggc gctgcagccc
     4021 gggcagggca gctccgcgct ctccacgtcg atcgtgtagc cgcacccctc gcataccagc
     4081 tggcgcgagc cgggaggtgc ggtgatgggc ccgccgcatt tcccgcaggt ccgctgcgag
     4141 gcatcgggga gcttcgcctt caggtactcc gattcaagcc ccgcgtactc gatgatctgc
     4201 tgcttggccg cgtcgaagag atacggcatc catccctgca ggaaggccga cgcccagccc
     4261 ttgatcatga tccgcggatc ggtttcgtcg gggtgtacga tggggacgtc ccaggtcgat
     4321 cgcttcttca tcgtttcaac cagtccctcg taattgaccc ggaagtagtt ccagacgctg
     4381 gccgagctgt atttcaggcc cgacggcgtc attgcgctct tgagcccgtt ataggcctcg
     4441 acgagcttca cgtcgtagat atgggccaag tcggcatcga acgccatgag cgcatcgacc
     4501 tgcgattggt tttcgatgag ggtggcccgg tatggcgggt caccgatgcg caccgaccag
     4561 gcatgggggc ttttctccat ctgcagcccg tataggcccc ggccgatctt gatgaatgcc
     4621 ttcttgtcgc cggcggcctt cgcgcgctca cgggccttgt ggaatttttc gcagtcggcg
     4681 gaatacttct cgtcgtacat ctgtcccgtg aacgtcaggg cgctttgcat gtcgaaatcc
     4741 atgtagccgc cgcagtagtc gcagtagaca tacgcgtatt gggcggcggt cgccttggcc
     4801 gccccacacg atgggcagat cgttttcatt accagctttg ttcttttatc catgaggtgt
     4861 ttcgtattca gcgttcggta tgtgatggtc gcgtgttgag cgcatggtgc tttagcgatg
     4921 ctgcaagtac gaaacacgtg attagacgtg atgtcaagat gattttatcg tgcatatagt
     4981 gtttgacaat tcctccgttt gaggcatcat cttggccgcc gggggcgtta tgaagtattc
     5041 ccccgagtgt ttcttacagg aggcgcgtcc atgtggtcat tcatcgtatt tctcgtcatc
     5101 ggaatcatcg ccggctggct tgccggcctc atctttaaag ggcggggctt tggtctcatc
     5161 tgggacatgg tcattggcgt cgctggttcc tttatcggcg ggtttgtttt taagcttatc
     5221 ggggtgggcg cgtacggatt tctcggatca gtcttcacgg cggtcgtggg ggcgctgcta
     5281 ctattgttca tcatcaattg gatcaagaag tagcaccggg ttggcggtat tgctgagatg
     5341 tgtgcacctt gaacatcatc gattgcgaca ttcgctctaa tctccccgac gaggtgacct
     5401 cgttcaatga atatggcaac ggcgatctca ccagagattt tccgccagag gtcgcctcgt
     5461 tggacaacgt tgtcgatgac gactaaccgg tgaagcagga tcgccagctg cggagctgcc
     5521 ctcgctgaag tacgctcgat cgcgcgcgcc ggttttatcg cgaacatgcg tcacttgccg
     5581 ggagggtcta gttttctaac agaaaagtac ggggaggaat tggcgagaaa attgaaggcg
     5641 gtgtgagggt gtttagcgaa ccataagccg ctcgtacact tcggggtggg attacccgct
     5701 cattggcttt gaccccaatt tcctgttggc cccgaatcct gcaagcgctc catgaatgta
     5761 actacaactg aaaccgcgtt cctttgcgcg cggggacaga atcatcgcat gatgacgcgg
     5821 tttattgaat cggtcggcac gcttatgatg ccgttcgtgg tatggatccg ggcgttcgtt
     5881 ttcgactggc tgatgatggc gccaacaata acgctgccgt cctcgagata tacctgctcg
     5941 agcttgttgt agtggtctcg aatctgcttt tcggttttga ggaccaggac aacggttttt
     6001 tgcccgtccg tgtcgatctt ctcggttatg gtgatttctt tgacgatgtc cttcttgacc
     6061 tcttcaatcg attcgggaac gtccttgtac atcggttcga tgacaatgat cgcgcgctgg
     6121 tgcagttgct tcacttcctt gatcagcgcc tcaagcgttt gcccttcaag aggacgcttt
     6181 atcgcggtaa cgttctcccg cgaggctccc tcgcccgttg cgatgcccga cgtgacggtc
     6241 atacactcgc tcccctttat gtcggcgctg tgcggcttgt cattatcctg ata
//
LOCUS       DAORHF010000214         6290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12434_length_6290_cov_5.531836, whole genome shotgun sequence.
ACCESSION   DAORHF010000214 DAORHF010000000
VERSION     DAORHF010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_6290_cov_5.531836"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /gene="hypD"
                     /locus_tag="PKJ16_14680"
     CDS             complement(<1..1037)
                     /gene="hypD"
                     /locus_tag="PKJ16_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HNV48288.1"
                     /translation="MNNETVHKLADALRNHRLDAPMRIMEVCGTHTTEFFRSGVRDIF
                     PAHLTLVDGPGCPVCVTANAYLDTAIEIGRAHGVIIATFGDMMKVPSSYSSLAEEKAT
                     GMDVRVVYSPMDALEIARHDPAREVVFVSVGFETTAPTEAATVIAARDAGIRNFSILP
                     GNKLTVPAVEALLSLGEVHIDGFILPGHVSAIIGSEAWRPIPERFGKPCVVAGFETAD
                     LITGTLALMKMIASNDRTVANEYARVVRPGGNPTALSMMRRVFEPDDAEWRGIGVIPG
                     SGLAVRDEFSRYDGFKKFPVTPPPVKEHPGCRCGELLRGLIAPTDCALFGAACRPERP
                     VGPCMVSTEGP"
     gene            complement(1027..3318)
                     /gene="hypF"
                     /locus_tag="PKJ16_14685"
     CDS             complement(1027..3318)
                     /gene="hypF"
                     /locus_tag="PKJ16_14685"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HNV48289.1"
                     /translation="MIRSVNIRVQGIVQGVGFRPFAYALARAHGIAGWVQNDTEGVLI
                     HATGDDSSVTAFIAAIDDKRPPLASIRSIDVIDTEPTGEIDFTIRESADTGTRAAFIP
                     PDTALCEDCRREHFDTADPRLHYPFITCTHCGPRFSIIRDIPYDRANTAMAPFPMCPR
                     CTAEYRDPDNRRFHTQPNACPICGPHLSLFDSSRTRVCAGTDEIARRVIGLIRDGAIV
                     AIKGVGGYLLAADARNDAAVRALRERKGRPFKPFALMAGDIDIVESFLEVSDVARDLL
                     LSRERPIVVLRERRETVSRLVAPGLSHVGVMLPYAPFQHLFFSLAPDMVLIMTSGNLS
                     EEPIVFDDDEAFERLGGIADYFVTYDRAIHAHSDDSVLFVADGAPHFIRRARGYVPVP
                     FRSRPSGRHIIATGGDIKNAFALAKDDIVIVSQHLGDLESPAGNALFQSCVAHFRTIF
                     DFAPDTVACDLHPGYFTTRIAEEFEARGMRRIGVQHHHAHIAAVMEEHDLDGPVIGIA
                     FDGTGYGADGTLWGSEFLIATRSGFERAAHFDYVPLPGGESAIHEVWKIGVSMLHAAR
                     GQSLPRLGERPGADSVMELIEKRINSPLACSIGRLFDGAAAILGIADTVSCEAEAAML
                     LEEAALRGSRVSRPDAFALPTENADGIIISTAALTEYLLDLLRNGAPVDVIAYRFHET
                     IADSAASVADMLRDRHGVNAVALSGGVFQNRLLLSLLLDRLRNKRFDVYPHRAVPSND
                     GCIALGQIAVAKERLRTGNANEQ"
     gene            3471..3794
                     /locus_tag="PKJ16_14690"
     CDS             3471..3794
                     /locus_tag="PKJ16_14690"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HNV48290.1"
                     /translation="MEDRDYIIRTLGAIAEENGAELAILFGSYGRGTASRRSDVDVIF
                     VEQTDDPFLRRIDRYLSPIIDRLRLAADVFVYTPKEFMRMRESFFVRKAISDGVVVFE
                     RRKVS"
     gene            3772..4182
                     /locus_tag="PKJ16_14695"
     CDS             3772..4182
                     /locus_tag="PKJ16_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNV48291.1"
                     /translation="MSGEKYREDAGRWYRQAQADLKAARGSMNTGSHEWASFQAQQAA
                     EKALKALWYFSGADPWGHSIAKLILDLPEGPYRDVVMPLLDSAKALDKLYIPTRYPNG
                     LPDMIPAEVYTPAESRSAIACSEAILYAVENAMR"
     gene            complement(4198..5211)
                     /locus_tag="PKJ16_14700"
     CDS             complement(4198..5211)
                     /locus_tag="PKJ16_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48292.1"
                     /translation="MKKQPEKIKIKQPPLLFDKTQKIIAKAEKILGCKTISYWTSDRG
                     SVCANDVVALYELFQKIGPGDRASLFIKSGGGNPEAALRIVNLIREYYRHVTAVIPLE
                     CASAATMIALGADHLQMGPLAHLTAIDSSLIHDLSPLDERENRRVSVSQDQVSRIVNL
                     WNKNAKDHHANPFSDIFKYIHPLVIGEIDRSSSLSIKIAKEILLYHLTDESEAERISR
                     HLNFDYPSHSYPITSKEASKIGLSVSALDSQANELLIELNELYSEMAQKAFTDFDEYN
                     YHDNEILNILEADGIQIYYQNDKDWNYLKEERRWQPMNDESSWRKIESVKGKTVHTQF
                     HIR"
     gene            complement(5306..6139)
                     /locus_tag="PKJ16_14705"
     CDS             complement(5306..6139)
                     /locus_tag="PKJ16_14705"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV48293.1"
                     /translation="MPNTFEIKEIPPIQDVVLKVVRYELDNEISSSNELLNIITPDKG
                     LSSEILRVANSAYYAGVGKVENLQTALNRLGLRTIKMLVMSAANSNIKAILRKGIYEK
                     YIHQYPLVTAFIAVDLLKTARRTDINKDDIFIEGLLAKIGMTVIALNAKKEYAQVLET
                     AEKKRMALTAVEEAAFKTNHIAVGAALARDWNLGDSIIEVIENPITDAAAVTGASDRV
                     RIIACADIIARELTGCLVLEPELAAKSAIAAHYGLAPEALDAFNAGYLAALQENPLFT
                     I"
BASE COUNT         1053 a         1852 c         2154 g         1231 t
ORIGIN      
        1 cagggccctt cggtggacac catgcagggg cccacgggcc gctccggacg gcaggccgcg
       61 ccgaagagcg cgcagtcggt cggggcgatg aggccgcgca ggagttcgcc gcatcggcac
      121 ccggggtgct ccttgacggg cggcggcgtc acggggaatt tcttgaatcc gtcgtagcgg
      181 gaaaattcat cacggacggc aaggccgctc cccgggatca cgccgatccc gcgccactcg
      241 gcgtcgtcgg gctcgaagac gcggcgcatc atactaaggg ccgtcgggtt gccgcccggg
      301 cgcaccacgc gggcgtattc gttcgcgacc gttcggtcgt tcgatgcgat cattttcatg
      361 agcgcaagcg tacccgtgat gaggtcggcc gtctcgaatc cggcgaccac gcagggcttt
      421 ccgaaccgct cggggatggg gcgccacgcc tcggagccga tgatcgcgct tacgtggccg
      481 ggaaggataa aaccgtcgat gtgaacctcg cccagggaca gaagcgcttc gacggccgga
      541 accgtgagct tgttgccggg gagaatcgag aagtttcgga tgcccgcatc gcgtgccgcg
      601 atgactgtcg cggcctcggt gggggccgtg gtctcgaatc caacgctcac gaatacgacc
      661 tcgcgagcgg ggtcgtgccg ggcgatctcc agcgcgtcca tgggcgagta cacgacacgg
      721 acgtccatgc cggtcgcctt ctcctccgca agcgagctgt acgatgaggg gaccttcatc
      781 atgtcgccga aggtggcgat gatgacgccg tgcgcgcgcc cgatctcgat tgccgtgtcg
      841 aggtacgcgt tcgcggttac gcacaccggg cagccgggtc cgtccaccag cgtgaggtga
      901 gcgggaaaga tgtcgcgcac gccgcttcgg aagaactccg tggtatgcgt tccgcacacc
      961 tccatgatgc gcatcggggc gtcgaggcgg tggttccgca gcgcgtctgc gagtttgtgt
     1021 accgtttcat tgttcattcg cgttccccgt tcgcagccgc tccttggcga cggcgatttg
     1081 ccccagcgcg atgcagccgt cgttggaggg aaccgcgcga tgcggataga cgtcaaatct
     1141 tttattgcgc agccgatcga gaagaagcga aagcagcagc cggttctgga atacgccgcc
     1201 cgacagcgcg acggcgttca cgccgtgtcg atcgcgcagc atgtccgcga ccgacgccgc
     1261 gctgtcggcg atcgtttcgt ggaagcggta ggcgatcacg tcgaccggcg ccccattgcg
     1321 cagtaggtcg agaaggtatt cggtcagcgc ggcggttgat atgatgatgc cgtctgcatt
     1381 ctccgtcggc agggcgaacg cgtccggccg cgatacgcgc gatccccgca gcgccgcctc
     1441 ctcgaggagc atcgccgcct cggcctcgca gctcacggtg tccgcgatgc ccaggatggc
     1501 cgccgcgccg tcgaacaatc ggccgatgct gcaggcgagc ggggagttga tccgcttttc
     1561 gatgagctcc atcacggaat cggcgccggg ccgctcgccg aggcggggga ggctctgacc
     1621 ccgggccgca tggagcatcg agacgccgat cttccatacc tcgtggatcg cgctctcgcc
     1681 gcccgggagc gggacgtagt cgaagtgcgc ggcgcgctcg aacccgctcc gcgtggcgat
     1741 cagaaactcg cttccccaga gcgtgccgtc ggcgccgtaa cccgtcccgt cgaaggcgat
     1801 gccgatcacc ggtccgtcca ggtcgtgctc ctccatgacg gccgcgatgt gggcgtgatg
     1861 gtgctggacg ccgatgcgcc gcatgccgcg cgcctcgaac tcctccgcga tgcgcgtggt
     1921 gaagtagccc ggatgaaggt cgcaggcgac ggtgtcgggc gcgaaatcga agatcgttct
     1981 gaagtgcgcc acgcacgatt gaaagagcgc gttgccggcc ggggattcga ggtcgcccag
     2041 gtgctggctc acgatgacga tatcgtcctt cgcaagggcg aaggcgttct tgatgtcgcc
     2101 gccggtcgcg atgatgtggc ggccggacgg gcgcgagcgg aagggcacgg ggacatagcc
     2161 ccgggcgcgt cggatgaaat gcggcgcacc gtccgcgaca aagagcacgc tgtcgtccga
     2221 gtgcgcgtgg atggcgcgat cgtaggtgac gaaatagtcg gcgatgccgc ccaggcgctc
     2281 gaaggcctcg tcgtcgtcga agacgatggg ctcctcggag agattgccgc tggtcatgat
     2341 gaggaccatg tccggcgcga gcgaaaagaa caggtgctgg aagggcgcgt agggcagcat
     2401 gacgccgaca tgggacaggc cgggcgcgac caggcggctc acggtttcgc gccgctcgcg
     2461 gagcaccacg atggggcgtt cgcgcgagag cagcagatcg cgcgcaacgt ccgagacctc
     2521 gaggaacgat tcgacgatat cgatgtcccc cgccatgagc gcgaatggct tgaacgggcg
     2581 tcccttgcgc tcccgcagcg cccgcaccgc cgcgtcgttt cgcgcatccg ccgcgagaag
     2641 atagccgccc acgcccttga tggcgacaat cgcgccgtcg cggatcagcc cgataaccct
     2701 gcgggcgatc tcgtcggttc ccgcgcatac acgcgttcgt gaggagtcga acagcgacag
     2761 gtgcgggccg caaatcgggc aggcgttcgg ttgcgtgtgg aagcgccgat tgtcgggatc
     2821 gcggtattcc gcagtacagc gcgggcacat ggggaagggc gccatcgcgg tgttcgcacg
     2881 gtcgtagggg atgtcgcgga tgatgctgaa gcgcggcccg cagtgcgtgc aggtgatgaa
     2941 gggatagtgg agccgtggat cggcggtatc gaagtgctcg cgacggcagt cctcgcagag
     3001 ggccgtgtcc ggcgggatga aggccgcgcg ggtcccggtg tcggcgcttt cgcgaattgt
     3061 gaaatcgatc tcgcccgtgg gttccgtgtc gatcacgtcg atcgagcgga tcgatgcgag
     3121 cgggggccgc ttgtcgtcga tggcggcgat gaatgcggtg acgctagaat catcccccgt
     3181 ggcgtgaatg agcacgccct ccgtgtcgtt ttgcacccag ccggcgatcc cgtgtgcgcg
     3241 cgcgagcgca tacgcgaagg gacgaaaccc cacgccctgc acaatgccct gtacgcggat
     3301 gttgacgctg cggatcatgc gattgtagtt ctcacgtgta ttcgcatcat tttccgctgt
     3361 atgacacggg gatggcaatt cattaatggc ggtttcgacc agaaaaggac ttgcaaaaac
     3421 aagcgttcat gccgtagtac ttatgtggtc ggatgaatcg gaacaacgat atggaagatc
     3481 gggattatat tatcagaacg ttgggggcga tcgcggagga gaacggcgcc gagctggcga
     3541 tcctgttcgg ctcctatggc cgggggaccg cctcgcgccg ttccgacgtc gatgtcattt
     3601 tcgtggagca aaccgatgat ccgttcctgc ggcggatcga tcgctatctt tctccgatca
     3661 tcgaccggtt gcgcctggcc gcggatgttt ttgtgtatac accgaaggag ttcatgcgca
     3721 tgcgggagtc tttcttcgtg cgaaaagcca tatcggacgg ggtggtggtc tttgagcggc
     3781 gaaaagtatc gtgaagacgc cggacgctgg tatcgccagg cgcaggcgga cctgaaggcc
     3841 gcccgcggga gcatgaatac cggcagccat gaatgggcgt cctttcaggc gcaacaggcg
     3901 gccgagaagg ccctcaaggc gttatggtat ttttccggtg ccgatccctg ggggcacagc
     3961 atcgcgaagc tcatcctcga tcttcccgag ggcccgtatc gggatgtggt catgccgctc
     4021 ttggactcgg cgaaggcgct cgacaagctc tacattccga cccggtatcc gaacggtctt
     4081 ccggacatga tccccgccga ggtgtacacg ccggcggaaa gccggtcagc gatagcgtgt
     4141 tccgaggcca tactgtacgc cgtcgaaaac gccatgcgat aggcatcgcc gcggttccta
     4201 tcgaatgtgg aattgcgtgt ggaccgtctt gcccttcacg gactcgatct tgcgccagga
     4261 gctctcgtcg ttcatcggct gccagcggcg ctcttccttg agataattcc aatccttgtc
     4321 gttctggtaa tagatctgga tgccgtcggc ctcgaggatg ttgaggatct cgttgtcgtg
     4381 gtagttgtac tcgtcgaagt ccgtgaacgc cttctgcgcc atctccgagt agagttcgtt
     4441 aagctcgatg aggagctcgt tcgcctggct gtcgagcgcc gagacgctca ggccgatctt
     4501 gctcgcctcc tttgaggtga tggggtagga gtgcgagggg tagtcgaagt tgaggtggcg
     4561 gctgatgcgc tccgcctcgc tctcgtccgt gagatggtac aggaggatct ccttcgcgat
     4621 cttgatcgac agcgagctcg aacgatcgat ctccccgatg accagcgggt gaatgtactt
     4681 gaagatgtcc gaaaacggat tcgcgtgatg atccttcgcg ttcttgttcc ataaattcac
     4741 gatgcgcgac acctggtcct ggctcacgct cacgcgccgg ttctcgcgct cgtccagcgg
     4801 cgagaggtcg tggatgagcg aggagtcgat ggccgtcagg tgcgccagcg gtcccatctg
     4861 caggtggtcg gcgcccaggg cgatcatggt ggcggccgag gcgcattcga gcgggattac
     4921 ggcggtcacg tgccggtagt actcgcggat gaggtttacg atgcgcaggg ccgcctcggg
     4981 attgcccccg cccgatttga tgaagagcga ggcgcgatcg ccgggcccga tcttctggaa
     5041 gagctcgtag agcgccacca cgtcgttggc gcataccgag ccgcggtcgg aggtccagta
     5101 ggagatggtc ttgcagccga ggatcttctc ggccttcgcg atgatctttt gggttttgtc
     5161 gaacaggagc ggcggctgtt tgatcttgat tttttccggt tgtttcttca tttgcatcca
     5221 tccggtcgga gtatgggcga cgacagaaag cacgccgggc cggcgcgtgt cctgtcaatc
     5281 aactttcggc gggcggtcgg gcgcgttata tggtgaacag cggattttcc tggagcgcgg
     5341 cgaggtagcc ggcgttaaag gcgtcgagcg cctccggggc gaggccatag tgcgcggcga
     5401 tggcggattt cgcggccagc tcgggttcca gcaccaggca gccggtgagc tcgcgcgcga
     5461 tgatgtcggc gcaggcgatg atgcggacgc ggtccgacgc gccggtgacc gccgcggcgt
     5521 ccgtgatcgg attctcgatc acctcgatga tcgaatcgcc caggttccag tcgcgcgcga
     5581 gcgcggcccc cacggcgata tggttcgtct tgaaggccgc ctcctcgacg gcggtgaggg
     5641 ccatgcgctt tttctcggcc gtctcgagca cctgcgcgta ttccttcttc gcgttgagcg
     5701 cgatcaccgt catgccgatc ttcgccaata gcccctcgat gaagatgtcg tccttgttga
     5761 tgtccgtgcg gcgggcggtc ttcaagaggt cgaccgcgat aaaggcggtc acgagggggt
     5821 actggtggat gtatttctcg tagatgccct ttcgcaggat ggccttgatg ttgctgttcg
     5881 cggccgacat gaccagcatc ttgatggtgc gcagccccag gcggttgagc gcggtctgca
     5941 ggttttccac cttgcccacg ccggcgtagt acgcggagtt ggcgacgcgc aggatctcgc
     6001 tggagagccc cttgtcgggg gtgatgatat tcaggagttc gttcgagctg gagatctcgt
     6061 tgtcgagctc gtagcgcacc accttgagca ccacgtcctg aatgggcgga atttccttta
     6121 tttcaaatgt gttcggcatc gcgtgtcgtt cctcgggtgc gcgtctcggt cgtgtgcgtc
     6181 gcaggctgtg cgccgctgtg gcgcatgaag gctttcacgc gggcgtatga tccgtcaatc
     6241 cattttcgcg cgactctgcg tcgaattcgc atggagtgca tgcggcgggg
//
LOCUS       DAORHF010000215         6268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12504_length_6268_cov_4.847739, whole genome shotgun sequence.
ACCESSION   DAORHF010000215 DAORHF010000000
VERSION     DAORHF010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6268
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12504_length_6268_cov_4.847739"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PKJ16_14710"
     CDS             <1..721
                     /locus_tag="PKJ16_14710"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48294.1"
                     /translation="NAIMDPSRDRPRASVIFGAIGLGSSNDYHKPMDQSPRIADVPAH
                     IDDKNARLVDVGKAVMVDTAGKKIVRYFILNASIGFVAEGNAYFNTNKPVLAWFKRRN
                     TDLAILYTIIVMLCTYKNIKASISLDGGIRKRYTITSLGILKKVQFSGDFVYDTPVTA
                     DDGMFDVNLWENMGRIGILGTLAALSKGKFTGRKKTRTWRAQSVEIAPDRPIHLELDG
                     ETTEIVSAELSVVHKAVKICW"
     gene            726..1571
                     /locus_tag="PKJ16_14715"
     CDS             726..1571
                     /locus_tag="PKJ16_14715"
                     /inference="COORDINATES: protein motif:HMM:NF025052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV48295.1"
                     /translation="MDTRTIAIVGIDGSGKTTVIRRMLERLPSRWDVVGLINCPDYHE
                     IPNAPLAQLSRDLDAFSQIADGLGDFQLKGVALYLQMTLYGPLSRFVVDTFHPRLLLS
                     ARHAVFDSLAYGSLYANIMKKPLDRARHEAALRERMEQYRAGSYDALLQWSAHIARRA
                     GCDEEFWRLDRHVLEVFSNSGRELVTVLEREYQTTLPDAVIFMDTPAETAQARIEGRQ
                     EKTQELHERSDMLAMIRRNYLDALSLLHVAYPDIETHVVNTGDGGGIDHTVDEVIRRA
                     NIDVS"
     gene            1649..1825
                     /gene="rpsU"
                     /locus_tag="PKJ16_14720"
                     /pseudo
     CDS             1649..1825
                     /gene="rpsU"
                     /locus_tag="PKJ16_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
     gene            2024..3313
                     /locus_tag="PKJ16_14725"
     CDS             2024..3313
                     /locus_tag="PKJ16_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HNV48296.1"
                     /translation="MTNAGEQFLPLSERCRPSTIEEFFGQRHLLDGGKIVRTMLERRA
                     PFSLILWGDPGTGKTTLARIIAAQCAMDAHHLSAVSAGVADVRAVIETGKKNRANGVR
                     TLLFLDEIHRFNKAQQDSVLGALESGDIVLIGATTENPSFQVIAPLLSRTRVLRLHPL
                     ADDDLRAILERALSRDPVLSAAHPVAEEAKALLVRFALGDARRLLNILEAAWSLAADG
                     SIGEHEIREAVSSTMLYYDRAGDRHYDSISAFIKSLRGSDPDAAVYYLARMLASGEDP
                     VFIARRMVIFAAEDVGNASPTAVSIAVAAFTAVKNIGMPEARIILSQCATFLAASPKS
                     NAAYRAIGEAMEAAARTDAPIPTHLRNAPTALLKEMGHGAGYRYPHDYPGHFIDEHYL
                     PEPLRGHVFYRPGEEGSEKAIGQRLRNLWPGRYDKER"
     gene            3316..3888
                     /gene="pyrE"
                     /locus_tag="PKJ16_14730"
     CDS             3316..3888
                     /gene="pyrE"
                     /locus_tag="PKJ16_14730"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HNV48297.1"
                     /translation="MAHAERDQLLELLYTRSFIYREDPPFTLVSGRESFYYFDCKATT
                     LTPQGLYLVGRLMFEQIAGSGAEAIGGLTLGADPIALATALEAYRNDTNITPLIVRKE
                     PKKHGTQKWIEGNLENVQNVIVVDDVITTGGSTITAVERMRESGLAVTKALVIIDREE
                     GGRESIEALGIPVVSLFTRSDFDRLRLSRR"
     gene            3996..4445
                     /locus_tag="PKJ16_14735"
     CDS             3996..4445
                     /locus_tag="PKJ16_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48298.1"
                     /translation="MRIGMTMFAFVVALLVACATQPKECVDGNCIDGKGKAKYNSGDH
                     YVGEFKGTQRGGQGEYNWKTGDLYKGAWKDGKRSGSGEYKYSVGDVYKGDFNNDNRHG
                     KGTYTWKSGEKYEGEWKDGKMDGAGTYTAPDGTKRTGKFEKGRYIGQ"
     gene            4523..5998
                     /locus_tag="PKJ16_14740"
     CDS             4523..5998
                     /locus_tag="PKJ16_14740"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HNV48299.1"
                     /translation="MNHKNLLGVAAAIVMGLQGLAGVAHADAIKKAPYAIERKSEYRQ
                     DFLIEMREFSGETDPTRAYLKKLVANILRDRIRIYKSIKVPRPILEFDRKTHNVDAPS
                     KQDGERFTHLAIRFVDDPKDAKPKQRDAVYAPVGNRAPDVTIAGTVTYRGDRLSVGVT
                     VDNRFHDAAGTVTHAGTLKEINVLIDRLAVDVMKLIIHNYGYLTVHSIPKGAGLYVDN
                     RYFGKTDVENLVIESGDHRIEVRTEDQLEAESAVTVPTHGVISVTLELQQLLKPGDRT
                     IRVVTNPEKASVYLDSRLVGVSPLEIKNLRAGTYRLRVAKDGHVTKFKTIKLDELKET
                     TIDFSLEPGRDEDYYFSRTTWYMTAFKVSLIGAAVCAVSSFYLDIRMEDERAKVRGFS
                     FKDPNNPTPEERNSYDDLKRRADERIDTYRLYQTYSIYAAVTLLVSAGVFYLLDVRQY
                     DIDIAFYWDPGLPRPEDARRPAVQAQSIGFRSAGVALTYFF"
     gene            complement(5981..>6268)
                     /locus_tag="PKJ16_14745"
     CDS             complement(5981..>6268)
                     /locus_tag="PKJ16_14745"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HNV48300.1"
                     /translation="REVMGHDSGFLNKINSFFMGMAQLFSQYITKAQDEGRIIKSIDP
                     TITSYSIIGSVKEVLFHWAVMDEKLDIQKSINTMIEIYFTGLIEQPSKEVR"
BASE COUNT         1310 a         1840 c         1933 g         1185 t
ORIGIN      
        1 caacgccatc atggacccgt cacgcgaccg gccgcgcgcg agcgtcatct tcggcgcgat
       61 cggcctgggc agcagcaatg actatcacaa gccgatggac cagtcccccc gcatcgcgga
      121 tgttcccgcg cacatcgacg acaagaacgc ccgcctggtt gacgtgggca aggccgtcat
      181 ggtggacacg gcgggcaaga agatcgttcg ctattttatc ctcaacgcca gcatcggctt
      241 cgtcgccgaa gggaacgcct atttcaacac caacaagccg gtgctcgcct ggttcaagcg
      301 tcgcaatacc gacctcgcga tcctgtatac catcatcgtc atgctctgta cctataagaa
      361 catcaaggcg tcgatttccc tcgatggcgg gatccgaaag cgctacacca tcaccagcct
      421 cgggatactg aagaaggtgc aattctcggg cgatttcgtc tacgacaccc ccgtgaccgc
      481 cgacgacggc atgttcgacg tgaacctgtg ggagaacatg ggacggatcg gcatactggg
      541 gacgctcgcg gcgctgtcga aggggaaatt caccggcagg aagaagaccc gcacctggcg
      601 cgcgcaatcg gtcgagatcg cgcccgatcg ccccatccac ctcgagcttg acggcgagac
      661 gacggagatc gtctccgcgg agctctcggt ggtccacaag gccgtgaaga tttgctggta
      721 gggccatgga tacgcgcacg atcgcaattg tcggcatcga cggatcgggg aaaaccaccg
      781 tcatcaggcg catgctggag cggctcccgt cgcgctggga cgtggttggc ctcatcaact
      841 gccccgatta tcacgagatc ccgaatgcgc cgcttgcgca gctttcgcgc gatctcgacg
      901 ccttctcgca gatcgcggac ggcctgggcg acttccagct caagggcgtc gcgctctatc
      961 tccagatgac gctctacggg ccgctgtcgc gctttgtcgt ggacaccttc catccgcgcc
     1021 tgttgctgag cgcgcgacac gcggtgttcg attcgctcgc gtacggatcg ctctatgcga
     1081 atatcatgaa aaagccgttg gaccgcgcgc gacacgaggc ggcgctccgc gagcggatgg
     1141 agcagtaccg cgcgggatcg tacgacgcgc tgttgcagtg gagcgcgcat atcgcgcgcc
     1201 gcgccggatg cgacgaggaa ttctggcgtc tggaccggca cgtgctcgag gtgttttcca
     1261 attccgggcg ggagctggtg accgtgcttg agcgtgaata tcagacgacc ttgcccgacg
     1321 cggtcatttt catggacacg cccgccgaaa ccgcgcaggc gcgcatcgag ggccgtcagg
     1381 aaaaaacgca ggaactgcat gagcgctccg acatgctcgc gatgatccgc cgcaactatc
     1441 tcgacgcgct gtcgctcctg catgtcgcct atccggacat cgagacgcat gtcgtgaaca
     1501 ccggcgacgg cggcggcatc gaccacaccg tcgacgaggt cattcgacgg gcgaatatcg
     1561 acgtgagcta gacggaaaaa ttatattgac atgtttttcg ccctcgctac agtggcgaca
     1621 aaatattcca taaggggttg atggccgaat gccgaatgca cttgaagtag aggtacgggg
     1681 cgatatcgag atcgcgttta agaatctcaa gaagaagatg acctacgagg gtatcttcaa
     1741 ggagctcaag aaacgccggt attacgaaaa gccgagtgaa gagaagaagc gcaagcgcga
     1801 ggaggccgag cgccgacgcg tcaagcgtat gcgccgcatg tcgttttcca agagcggcgg
     1861 ttcctccggc ggcggcggtg gtggcggaca gaagcggttc atgaatatca agtttaacgc
     1921 ccccgaaagc ggcacgcagg aagagagcaa gtagcttcca aaaccaaatg agtgtttgaa
     1981 aaaatccggt tatgccggat ttttttatct cgatacctct tcaatgacaa acgccggtga
     2041 acagttcctt cccctctcgg agcgctgtcg cccctcgacg atcgaggagt ttttcggcca
     2101 gcggcatctg ctcgacgggg ggaagatcgt gcgcaccatg ctcgagcgcc gcgccccctt
     2161 ttcgctgatc ctgtggggcg atcccgggac ggggaaaacg acgctcgcgc ggatcatcgc
     2221 cgcgcagtgc gccatggatg cgcatcattt gagcgccgtt tcggccggcg ttgcggatgt
     2281 gcgcgcggtc atcgagaccg gaaaaaaaaa cagggcgaac ggcgtccgca ccctgctgtt
     2341 tctcgacgag atccaccggt tcaacaaggc gcagcaggat tccgtgctgg gcgcgctcga
     2401 gtccggcgat atcgtactca tcggcgcaac cacggaaaac ccgtccttcc aggtgatcgc
     2461 gccgctgttg tcgcgcacgc gggtgctccg cctccatccg ctcgccgatg acgatctgcg
     2521 cgcgatactc gaacgcgcgc tctcccgcga tcccgtcctg tcggcggcgc accccgtcgc
     2581 ggaggaggcg aaggcgctcc tggtgcgctt cgcgttgggc gatgcgcgac gcctgcttaa
     2641 tattctcgag gcggcctggt cgctcgccgc ggacggctcg atcggcgagc acgagatacg
     2701 cgaggccgtg agctcgacca tgctgtatta cgatcgcgcc ggcgaccgcc attacgattc
     2761 catctcggcc tttatcaaat cgctgcgcgg ttccgatccc gacgccgcgg tctactatct
     2821 cgcgcggatg ctcgcatcgg gcgaggaccc ggtgttcatc gcgcggcgga tggtcatctt
     2881 cgccgccgaa gacgtgggaa acgcgtcgcc caccgccgtg agcatcgccg tggcggcgtt
     2941 cacggccgtg aagaacatcg gcatgccgga ggcgcggatc atcctttcgc agtgcgcgac
     3001 cttcctggcg gcgtcgccga agagcaatgc ggcctaccgg gccatcggcg aggcgatgga
     3061 ggcggcggcc cggaccgacg cgccgatccc gacgcatttg cgcaatgccc cgacggcgct
     3121 gctcaaggag atgggccacg gcgcggggta tcggtatccc catgattatc cgggacattt
     3181 tatcgacgaa cactatcttc ccgaaccgct tcgcgggcat gtcttttacc ggccgggcga
     3241 ggaggggagt gaaaaggcga tcggccagcg gttgcgaaac ctctggcccg gccgctatga
     3301 caaggagagg tgaccatggc acacgccgaa cgcgaccagt tgctcgagtt gctgtacaca
     3361 cgttcattta tctatcgcga ggacccgccg tttacgctgg tgtcgggacg cgagagcttt
     3421 tactatttcg actgcaaggc gaccacgctc accccgcagg gcctgtacct tgttgggcgc
     3481 ttgatgttcg agcagatcgc cgggtcgggc gcggaggcga tcggcggact caccctcggg
     3541 gccgatccca tcgcgctggc gaccgcgctc gaagcgtatc ggaacgacac gaacatcacg
     3601 ccgctgatcg ttcgcaagga gcccaagaag cacgggacgc agaagtggat cgagggaaat
     3661 ctggagaacg tacaaaacgt gatcgtcgtc gacgatgtga tcacgacggg cggatcgacc
     3721 atcaccgccg tcgaacgcat gcgcgagtcg gggcttgcgg tgacgaaggc gctggtgatc
     3781 atcgatcgcg aagagggcgg ccgcgagagc atcgaggcgc tgggcattcc ggtcgtttcg
     3841 ctgttcacgc gctccgattt cgatcgcctg cgcctgtcga ggcggtaatt tattgacgtt
     3901 ttaataaaat ttcttcttgc aacgatgatt cggctatatg atgtacttac tatggaattg
     3961 ttgattaaca gttataaaaa agtgtggagg atgatatgag aattggtatg acgatgttcg
     4021 ctttcgttgt cgctctattg gttgcttgcg cgacacaacc gaaggaatgc gtcgatggca
     4081 attgcatcga cggcaaggga aaggcgaaat acaatagcgg cgaccattat gtcggcgaat
     4141 tcaaggggac ccagcgcggc ggccagggcg agtacaactg gaagaccggc gacctctaca
     4201 aaggggcctg gaaggatggc aagcgctccg gttcggggga gtataaatac tccgttggcg
     4261 atgtctataa aggcgatttc aacaacgaca accgccacgg caagggaacc tacacctgga
     4321 aatccgggga aaaatacgag ggcgagtgga aggacggtaa gatggacggc gccggcacct
     4381 acaccgcgcc cgacgggacc aagcgcacgg gcaagttcga aaagggacga tatatcggtc
     4441 agtagtttcg accaggatcg taccgggacc gataaccctt ctcgccgttt ccggagaagg
     4501 gttattatta tccattcctt ctatgaacca taaaaatcta ttgggcgttg cggccgccat
     4561 cgtgatgggt cttcagggac ttgcgggcgt ggcgcatgcg gacgcgatca aaaaggcgcc
     4621 gtacgccatc gagcggaaga gcgagtatcg ccaggatttc ctgatcgaga tgcgggagtt
     4681 ttccggcgaa accgatccca cgcgggcgta tctgaagaag ctggtggcga acatcctccg
     4741 ggaccgcatt cggatataca aaagcatcaa ggtcccgcgg ccgattctcg aattcgatcg
     4801 taagacccac aacgtggacg caccatcgaa acaggacggc gagaggttta cgcaccttgc
     4861 gattcggttc gttgacgatc cgaaagacgc gaagccgaag cagcgggatg cggtgtatgc
     4921 tccggtgggg aaccgtgcgc ccgacgtgac catagcggga acggtgacct accgcggcga
     4981 ccgcctgtcc gttggcgtaa ccgtggacaa ccgtttccac gacgccgccg gaacggtcac
     5041 gcatgcgggg acgctcaagg agatcaatgt gctcatcgac cgtttagcgg tcgatgtcat
     5101 gaagctgatt attcataact acgggtattt gaccgtccac tcgatcccca aaggggccgg
     5161 actgtacgtc gacaaccgct atttcggcaa gaccgacgtc gagaacctcg tgatcgagtc
     5221 gggagaccac cggatcgagg tccggaccga agatcagctc gaggccgaat cggcggttac
     5281 cgtgcccacg cacggcgtta tatcggtgac cctcgagctc cagcaacttc tcaagccggg
     5341 ggaccggacc atccgggtgg tgacgaatcc ggaaaaggcc tcggtctatc tcgattcgcg
     5401 gctggtcggc gtgagccccc tcgagatcaa gaaccttcgc gcggggacat atcggcttcg
     5461 cgtcgcgaag gacgggcacg tcacgaaatt caagacgatc aagctggacg agctcaagga
     5521 aaccaccatc gacttttcgc tggagcccgg ccgggacgag gattactatt tcagccgcac
     5581 cacctggtac atgacggcct tcaaggtctc gctcatcggg gccgcggtgt gcgcggtctc
     5641 gtcgttctat ctggacatcc gcatggagga cgagcgtgcg aaggtccgcg gtttttcatt
     5701 caaggatccc aacaatccga ccccggagga gcgaaacagc tacgacgatc tcaagcggcg
     5761 cgccgacgag cggatcgaca cctatcgatt atatcaaacc tactccatct acgccgcggt
     5821 gaccctcctg gtgtcggccg gtgtgtttta tttgctcgat gtccggcagt acgatatcga
     5881 catcgccttc tactgggacc ccgggctccc gcgcccggag gacgcgcgcc gtcccgcggt
     5941 gcaggcgcaa tcgatcgggt tccgctcggc cggtgtggcg ctaacgtact tcttttgagg
     6001 gctgttcgat aaggcccgtg aagtagatct cgatcatggt gttgatcgat ttctgaatgt
     6061 ccagcttctc gtccatcacc gcccagtgga agagcacctc cttgaccgat ccgatgatgc
     6121 tgtaggaggt gatggtgggg tcgatggact tgatgatgcg cccctcgtcc tgcgccttgg
     6181 tgatgtactg cgagaagagc tgcgccatcc ccatgaagaa cgaattaatc ttgtttaaaa
     6241 accccgaatc gtggcccatc acctcgcg
//
LOCUS       DAORHF010000216         6235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12606_length_6235_cov_4.419903, whole genome shotgun sequence.
ACCESSION   DAORHF010000216 DAORHF010000000
VERSION     DAORHF010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12606_length_6235_cov_4.419903"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="PKJ16_14750"
     CDS             complement(<1..806)
                     /locus_tag="PKJ16_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48301.1"
                     /translation="MAEPLSPNAIIVIKARFGAPERSASGIALLYISPPQRRLLKSFA
                     VVASGDELAKTNVTMPCAELMDVLGLAHTSSDVFTETTAHLVDGFPHDFTDSAIGSVI
                     EGALTGNNLFVDELLKRSVKAVLAVDKVEVLSANETISTGRFEEIKVEDELDRMTFAD
                     QVNHASRYLGNKRDLAEQVTREKTRDLVVVQFAFNSEAARGGGMLFYNTLIRSLSALF
                     VWATREKIKGSIDVKEAPVDFHRRFGSLMGSVTPDGKIGDEFIARIRKIHA"
     gene            complement(817..1845)
                     /locus_tag="PKJ16_14755"
     CDS             complement(817..1845)
                     /locus_tag="PKJ16_14755"
                     /inference="COORDINATES: protein motif:HMM:NF025749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4421 family protein"
                     /protein_id="HNV48302.1"
                     /translation="MRVRPIALLAVFILCVIASMLPALARAADIDQFDDKIMIRPFVQ
                     YPISQLQIRNYDNSLDAVSSDTLTNLTTYQPNVNFNVGLGVGYKWFGLSYQMAVTGTA
                     RDEADYGRSEYIDFQFYFVFKKFGVDLFFQRYSGYYLVNPIRLDNPQDYNVTKGDAHV
                     KRSDLKLTSIGLNGYYIFNDNFSFKAAFKQTERQNTSAGSFMLMASAINYGIEAEHSL
                     IPPSQEVFYGENAGFSRGMFTALCVLPGYAYTFVFPNHVFITPAGFIGAGGMIKNYHV
                     TSGDKGETDFCWKFNVRIGAGYNGDTYFYGITAMYDRTDSQGFVSKGGISVAVAVGQI
                     EAYAGVRF"
     gene            2018..3292
                     /locus_tag="PKJ16_14760"
     CDS             2018..3292
                     /locus_tag="PKJ16_14760"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HNV48303.1"
                     /translation="MIIYRILLLIAPALLRIIAPFDAKLRRFRSLRIGEVERVRAAFR
                     NRPDLPVLWFHAASAGEFEQAKPIIESLNGTEAGKRYYIVASFFSPSGFDAGAKYPGV
                     DFIFNLPLDSATNARALLDAIGPCAIIYAKYDVWINLTLEAHHRGVKLFLVSGTLPEK
                     SLRHRFPMKYILRSTYASLTGIYAISEADARRYRAIAGPRCGEIESAGDTRFDRVRQV
                     IEEHAKKPLEIIARENGLLYFLAGSTYAISESKLLEAYAAVHAQRIRLIMVPHEIDPK
                     NIDRLAGLIASRGLQPIRYSQLAPPIAVDGDQVLIVDAFGVLAHLYRQADIVFVGGSF
                     KGSVHSVLEPAVFGCPIITGPHICNAYEAITMAHNGIIALCPGMRELAEALDRLASDP
                     AKREDIGRRTKAFFDDNTGATQIIVSKIAAHL"
     gene            3304..4098
                     /locus_tag="PKJ16_14765"
     CDS             3304..4098
                     /locus_tag="PKJ16_14765"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HNV48304.1"
                     /translation="MDARIRHRRRIAALLLATFLLAPGDRQAASQEKREVELTAQEIV
                     ARVDRIMNYPVGLIKGRMLHVSPDGKSSAIDFVANTTDTDFLFTFSTRDRGDSIRVLY
                     NFSGEDIWVYSIHAMRLFHKVGVDKYDPIYGTNFTFVDFSNADLQSNFTAQITGDAIV
                     KNMESYRLRLTPIYKGGQYGQLNLYVSKSDFVPLRIDYHDNDKVIFKTLSVVQTISKG
                     NRIIPTRYDMLDIKKGTVTIVTFFGFDEKASYDKSIFRHQTLGDRR"
     gene            complement(4099..5967)
                     /gene="htpG"
                     /locus_tag="PKJ16_14770"
     CDS             complement(4099..5967)
                     /gene="htpG"
                     /locus_tag="PKJ16_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HNV48305.1"
                     /translation="MSSHKFQTEVNQLLHLIIHSLYSHKEIFLRELISNAADALEKLK
                     YLTLTDDAFKSFPFDPRIDISFDKNSNILTVADTGIGMNETDLVENLGTIASSGTRKF
                     LEQITGDQKKDSGLIGQFGVGFYSSFMVADRIEVITRKAGEDKAYRWASDGKGEYQIE
                     EASRDGAGTTITLHLNENGAEYASRWQIEDIVKKYSNHIMFPIFLHYEDTTYEGEGDK
                     KKEKKENKVSQINQASALWKRSKSELKEEDYHGFYKTLSHDTEAPMLYTHTQAEGAME
                     YTTLFYVPAKAPFDLYYRDYRAGVKLYVKRVFITDDDRELMPTYLRFVRGIIDSEDLP
                     LNVSREMLQKNRILEKIKSASVKKILEELKKLAEDTERYAAFWKEYSRPMKEGLYMDF
                     ENREALLDLMRFKSTKADGFVSLAEYKDRMQTDQKAIYYITGEREANLRNSPLLEMYR
                     DKDVEVLIMDDEIDEVILPQVGRYKDIELKSVNRSDAAEDLKSEGDKKAEKEVEPLVK
                     RIKQVLGDRVKDVKASTRLSSSPSCIVADEKDPTVQMQGLLKAMGNADISSVKPILEI
                     NPAHDIVVRLKDVSDEAVFADISSLLYEQAMLIEGVKLENPGQFVERLNRVVAKAL"
BASE COUNT         1293 a         1857 c         1725 g         1360 t
ORIGIN      
        1 gcgtggatct tacggatgcg cgcaatgaat tcatcgccga tctttccgtc gggcgtgacg
       61 ctccccataa gcgacccgaa ccgccggtgg aaatcgaccg gcgcttcctt cacatcgatt
      121 gagcccttga tcttctctct cgtggcccag acgaataacg cgctcaatga tcggatgagc
      181 gtattataga agagcatccc gcccccgcgc gcggcctcgc tgttaaaggc gaactgaacc
      241 acaacgagat cgcgggtctt ctcgcgggtg acctgctcgg cgaggtcgcg tttgttcccg
      301 agatagcgtg acgcgtgatt cacctggtcg gcaaaggtca tccgatcgag ctcgtcctcg
      361 accttgatct cctcgaagcg accggtcgat atcgtctcgt tggccgaaag cacctcgacc
      421 ttatcgaccg caaggacggc ttttaccgag cgcttgagga gctcatcgac aaacagatta
      481 ttgcccgtca atgccccctc gatgacagaa ccgatggcgg aatcggtgaa atcgtgcgga
      541 aagccatcca cgaggtgtgc ggtggtttcg gtgaacacgt cggacgaggt gtgcgccagt
      601 cccaggacgt ccatcagctc cgcgcacggc atggtcacgt tcgtcttcgc aagctcatcg
      661 cccgatgcga caaccgcaaa gcttttaagc aaacgccgct gggggggact gatatacagg
      721 agcgcgatcc cggaggccga acgctcggga gcgccaaaac gcgccttgat gacgataatg
      781 gcgtttgggg aaagcggctc ggccatggct gcccggctag aaccggaccc ccgcgtatgc
      841 ctcgatctgt cccaccgcaa ccgcgacaga gatcccgccc ttagagacaa atccctgcga
      901 atcggtgcga tcgtacatcg cggtaatccc gtaaaaatac gtatcgccat tgtaccccgc
      961 gccgatgcgc acgttgaact tccagcagaa gtccgtctcc cccttgtcgc cggatgtgac
     1021 gtgatagttc ttgatcatcc cgccggcgcc gatgaatccg gccggggtga tgaacacgtg
     1081 gttcggaaat acgaaggtgt aggcgtagcc gggaagcaca cagagcgccg tgaacatccc
     1141 gcggctgaag ccggcgtttt ccccgtagaa cacctcctgg ctcgggggaa tgagcgaatg
     1201 ctccgcctcg attccgtaat tgatcgccga ggccatcaac atgaaggaac cggccgacgt
     1261 gttttgccgc tcggtctgtt taaaggcggc cttgaacgag aaattgtcat tgaagatgta
     1321 atagccgttc aggcctatcg atgtgagttt gaggtcggag cgcttgacgt gcgcatcgcc
     1381 cttggtgaca ttataatcct gtggattgtc gagccggatc ggattcacga ggtaatagcc
     1441 cgaataccgc tggaagaaga ggtccacgcc aaatttcttg aatacgaaat aaaactgaaa
     1501 atcgatgtac tcgcttcttc catagtcggc ctcgtcgcgg gccgtgccgg tcacggccat
     1561 ctggtacgag aggccgaacc atttatagcc gacgccaagc cccacgttaa aattcacatt
     1621 gggctgatag gtagtgagat tggtcagggt gtcggagctg acggcgtcca gcgaattgtc
     1681 gtaattgcga atctgcagct gcgatatggg gtactggaca aatggccgaa tcatgatctt
     1741 gtcgtcgaat tgatcgatgt ccgcggcgcg tgcaagcgcg ggaagcatcg acgcgatcac
     1801 gcacaggatg aagactgcaa gcaaagcgat gggacgaact ctcatggcaa cctcgacaac
     1861 ataccggtat ctgcggctcg cgcgcgcact aaagtatctt acattaacat atcgattatt
     1921 ccgtcaacat taatctacat tttcgaacgg ctttgtattg acaaaagcat agcgaatgat
     1981 agaaacgaat gcataccgtg catcatatgg tactccaatg atcatctatc gtattctgct
     2041 gctgattgcc cccgcgctgt tgcggatcat cgcgccattc gacgcgaagc tgcgccggtt
     2101 tcgctcgctt cgcatcggcg aggtggagcg cgtccgcgcc gccttccgga accgtcccga
     2161 tctgccggtt ctgtggttcc atgccgcctc ggccggcgag ttcgagcagg cgaaacccat
     2221 catcgagtcg ctcaacggaa ccgaggccgg aaagcgctat tatatcgtgg cgagcttctt
     2281 ctcaccgtct ggattcgacg cgggggcgaa atacccgggc gtcgatttca tcttcaattt
     2341 gccgctcgac agcgcgacaa acgcgcgcgc gcttctggac gccatcgggc cgtgcgcgat
     2401 catctatgcg aaatacgacg tctggatcaa cctcacgctc gaggcgcacc atcgcggggt
     2461 gaagctgttt ctcgtcagcg ggacccttcc ggaaaaatcg ctccggcacc gcttccccat
     2521 gaagtacatc cttcgatcga cgtatgcgtc gctcacgggc atctacgcga tctccgaggc
     2581 ggacgcacgc cgctaccgcg caatcgcggg gccacgctgc ggcgagatcg agtccgccgg
     2641 cgacacgcgc ttcgatcgcg ttcggcaggt catcgaggag cacgcaaaaa aaccgcttga
     2701 gatcatcgca cgggaaaacg gcctcctcta tttccttgcc ggaagcacct atgcgatatc
     2761 ggaatccaag ctccttgagg catatgccgc tgttcacgcg cagcgcatca ggctgattat
     2821 ggttccccat gagatcgacc cgaagaacat cgatcgcctc gccggcctga tcgcctcgcg
     2881 cgggctccag ccgatccgtt acagccagct cgctccgccc attgccgttg acggggacca
     2941 ggtcctcatc gtggacgcct tcggcgtcct ggcgcatctc taccgacagg ccgatatcgt
     3001 gttcgtcggg ggatcgttca aggggagcgt gcacagcgtg ctcgaaccgg cggtgttcgg
     3061 ttgtcccatc atcaccggcc cgcacatctg caacgcctac gaggcgatta ccatggccca
     3121 caacggcatc atcgcgctct gccccggaat gcgcgagctt gcggaggcgc tcgatcggct
     3181 cgcatcggac cctgcaaagc gcgaggatat cgggcggcgc accaaggcat tttttgacga
     3241 taacaccggg gcgacgcaaa ttatcgtttc caaaatcgcg gcccacctgt agactgagcc
     3301 ggaatggacg cccgaatccg acatcgccga cgcatcgccg cattgttgct cgcgacgttc
     3361 cttctcgccc ctggggaccg ccaggccgcg agccaggaaa agcgtgaggt cgaactcacc
     3421 gcgcaagaga tcgtggcgcg cgtcgaccgg atcatgaact atcccgtagg cctcatcaag
     3481 ggccgcatgc tgcacgtctc tccggacggg aaatcgtccg ccatcgactt cgtcgccaac
     3541 accacggata cggatttcct gtttacgttc agcacgcgcg accggggcga ctccatcagg
     3601 gtgctgtaca acttcagcgg ggaggacatc tgggtgtaca gcatccacgc gatgcgcctg
     3661 tttcacaagg tgggcgtgga caagtacgat cccatttacg ggaccaactt caccttcgtc
     3721 gacttctcaa acgccgacct gcagagcaac ttcacggcgc agatcacggg cgacgcgatc
     3781 gtgaaaaaca tggaatcgta ccggctgcgc ctgacgccga tctacaaggg cggccaatac
     3841 gggcagctca atctctatgt gagcaagagc gatttcgtgc cgctgcggat cgactatcac
     3901 gacaacgaca aggtgatctt caagaccctc tcggtggtgc agaccatatc gaaggggaac
     3961 cgtatcattc ccacccgcta cgacatgctc gacatcaaga agggaacggt gaccatcgtc
     4021 acctttttcg ggttcgacga aaaggcgtcg tacgacaaga gcatcttccg ccaccagacc
     4081 ctgggcgacc ggcgctagct acagcgcctt ggccaccacc cgattgaggc gctccacgaa
     4141 ttgcccgggg ttctccagct tcaccccctc gatcaacatc gcctgctcat agagaagcga
     4201 actgatgtcg gcgaacacgg cctcgtcgga cacgtccttc agccgcacca cgatgtcgtg
     4261 ggcgggattg atctcgagaa tgggcttgac gctcgaaata tcggcgttgc ccatcgcctt
     4321 cagcagcccc tgcatctgca ccgtggggtc tttctcgtcg gcgacgatgc aggagggcga
     4381 gctcgaaagc cgggtgctcg ccttgacgtc cttcacgcga tcgcccagca cctgtttgat
     4441 ccgcttgacc agcggctcga cctccttctc ggccttcttg tctccctcgc ttttgaggtc
     4501 ttcggccgcg tcgctgcgat tgaccgactt gagctcgatg tccttgtagc ggccgacctg
     4561 cggcaggatc acctcgtcga tctcgtcgtc catgatgagg acctcgacgt ccttgtcgcg
     4621 atacatctcc aggagcggcg aattccggag gttcgcctcg cgctcgccgg tgatgtagta
     4681 gatcgccttc tgatcggtct gcatccggtc cttgtactcg gccaggctca caaaaccgtc
     4741 ggccttggtc gacttgaagc gcatgagatc gagcagggcc tcgcggttct cgaagtccat
     4801 gtacagcccc tccttcatgg gccggctata ctccttccag aatgccgcgt agcgctccgt
     4861 gtcctcggcg agctttttca actcctctaa aatcttcttt acggacgccg acttgatctt
     4921 ttccaggatg cggttcttct gcaacatctc gcggcttaca ttcagcggca ggtcttcgga
     4981 gtcgatgatg ccgcgcacga aacgcagata ggtcggcatg agttcgcgat cgtcgtcggt
     5041 gatgaacacg cgcttcacgt agagcttcac gccggcgcga tagtcgcggt agtacaggtc
     5101 gaacggcgcc ttggcgggaa cgtagaagag cgtggtgtat tccatggcgc cctcggcctg
     5161 ggtatgggtg tagagcatgg gcgcctcggt gtcatgggac agggtcttgt agaatccgtg
     5221 ataatcctcc tccttgagct cgctcttgga acgcttccac agcgccgacg cctggttgat
     5281 ctgcgaaacc ttgttttcct ttttttcctt tttcttatca ccctcgcctt cgtaggtcgt
     5341 gtcctcgtaa tgcaggaaga tgggaaacat gatgtggttc gagtacttct tgacgatgtc
     5401 ctcgatctgc cagcggctcg cgtactcggc gccgttttcg ttcagatgga gcgtgatcgt
     5461 ggtcccggcc ccatcgcgcg aggcctcctc gatctggtac tcgcccttcc cgtcgctggc
     5521 ccatcgatac gccttgtcct cgccggcctt tcgggtaatg acctcgatgc ggtcggcgac
     5581 catgaaggac gaataaaacc ccactccgaa ctgcccgatg aggcccgagt ccttcttctg
     5641 atctccggta atctgctcga ggaacttgcg cgtcccggaa ctggcgatgg tgcccaggtt
     5701 ctccaccaga tcagtctcgt tcatgccgat cccggtatcg gccaccgtca gaatattcga
     5761 gtttttatcg aatgaaatgt cgattcgcgg atcgaagggg aatgatttga aggcgtcatc
     5821 ggtcagggtt agatacttga gtttttccaa agcgtccgcc gcgttcgata tgagctcccg
     5881 caagaagatc tccttgtgcg agtagagcga atggatgatg agatggagca gctgattcac
     5941 ttcggtctgg aacttgtgac tagacatctc ttaaacctct attatcgttt taaaatatac
     6001 aaatttaata attgtattat tagccatcag caccggcgag tgctgatttt attaatttaa
     6061 cattctcacc atcgatcgtc aagaggaaag ttattttttt atttcatttt ggtgaagact
     6121 tgattcgcac aatttttaca atcgtgcgca ccgagacaca tcttgcggct cggcatattc
     6181 atatatcaag accccagcgt caaatcaacg cgcgacgtgc acaaatcgaa aaagt
//
LOCUS       DAORHF010000217         6216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12652_length_6216_cov_4.473624, whole genome shotgun sequence.
ACCESSION   DAORHF010000217 DAORHF010000000
VERSION     DAORHF010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12652_length_6216_cov_4.473624"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..1305
                     /locus_tag="PKJ16_14775"
     CDS             16..1305
                     /locus_tag="PKJ16_14775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48306.1"
                     /translation="MDILLVEDNPGDARLIQEMLKEGRLAGSEVDVVERLDAALRRAG
                     ERRFGAIILDLNLPDSTGLDTLDRIVAATGGMIPVIILTGLNDEVMGESSINRGADDY
                     LVKGDVTLSQLARSLRYAVERKRMARELAEREERYRALVEHQGDAVCRWLPDTTLTYV
                     NGAYCALTGVSREHAAGRRWIDFVPAEDREAVLSFYCDLPDGLDVYTFEHRVMTPSGV
                     NRWIFWSDVPIRSVNGNIVEFQSVGRDITERKEAEDLLTSNFTLLRIAGETAKFGGWR
                     VDIASGIIFWSDEVAAIHEMPSGYCPSVNEGIDFYAPEWRDMITRIFTDCAVKGIPYD
                     EEMEIITAKGNRVWVRTIGEAVRDTNGKIIQVQGSFQDITERKRIEEKTMAQLEELRR
                     WHEAMLDREERVIELKREVNDLLKRLGEPARYRSVEE"
     gene            1325..2992
                     /locus_tag="PKJ16_14780"
     CDS             1325..2992
                     /locus_tag="PKJ16_14780"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV48307.1"
                     /translation="MKRNEYRTILDSLPVGILVLDEQVCVRDANDAAQRLFNASIDEL
                     RDRRCGDFVGCANRHDNERGCGYSSLCGSCALLNAVTEVFTGDKGVYDREVEFLIDRD
                     GAPVRAWMRFSVVPVVYDGSRCVVVAFNDISERKRAKEALEKSVEKYRTLTENSPDLI
                     ARFDRQLRHRYVNAAAAAAGTLSPEEYIGKSIGETGVPEPARSTWEKRIQAVFDAGNM
                     LDVVDSFPTPGGPRHFHTRLVPERDSAGSVQSVITIARDITERKLAEAQTIEAQAETQ
                     RLLREADRSRRALLSVIEDQKIVEKKLEETLSGLEQRVAERTAELVAANKELESYSYT
                     VSHDLRAPLRHISGFIDMLHQHMGPRLDVKGAHYMEVIVDSARFMGQLIDELLSFSRM
                     SRADMDVGRVDLNQLVGSIIKELTVENPGPVPTFELATLPAVPGDVALLRIAFVNLLS
                     NAVKFSSTKNDSRITIGAVSQDDTTMVVIRDNGVGFDMRYADKLFGVFQRLHAADEFP
                     GTGIGLATVKRIIERHGGSVRAEGMPGEGAAFYVSIPRCSHDAEEGK"
     gene            2992..4509
                     /locus_tag="PKJ16_14785"
     CDS             2992..4509
                     /locus_tag="PKJ16_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48308.1"
                     /translation="MNAKTLLLVDDSAHDVELFLASCGESALRERTDVARDGAEALDY
                     LYRKNAYRSRPPGNPYCVFLDIKLPKVSGIEVLAIMKGDSTLRHIPVIMLSSSREESD
                     MSTCYRYGANGYMVKPVMAGDLAPALTAACHFWLDINVVPHRPDSMPGTPVADGGASS
                     SMSDLRILHLEDNPRDCEMIHAEMARGGISSQPVVVSRREDYVKALSSVSPDVILSDY
                     LLPGINGLEALKLAQEHALDVPFIFISGAMGEEVAVETLKLGATDYVLKDKLKKLVPA
                     IRRALQESGERARRVAAEERLWGILHEREVLLKEIHHRVKNNMQVISSLLNIQKQQPA
                     RQSFDECMRSMHMRIRSMALVHERIYHAETLAAVDFAGCVRAIVDELVKSDAVDAKRI
                     DIACSVPPLTLPADIAIPAGLIATELISNALRHAFPENRNGAVSITMLTQGNGSIKLS
                     VVDNGVGIPMRFIHGGEGTLGLHLVEILVGQINGTIEISRDEGTAVSVLFMPMGG"
     gene            4510..>6216
                     /locus_tag="PKJ16_14790"
     CDS             4510..>6216
                     /locus_tag="PKJ16_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48309.1"
                     /translation="MIKKKILIVEDESINVMVLKGMLTGMGHEVVGVAETGREAIAMA
                     RGTVPDLVLMDIFLKDDVDGVEAAREINSFSSVPIIYVTAYSDRDTIERVKTTRSSAC
                     ILKPYELQQLQTTIDIALHRYTVEKQLRESEMRYRSLFESSRDAVYIRTLEGRIIDCN
                     EAMLSLFGYTTDEIMDLDVATAYVDVAVKDRLQIAITAQEAVRDFPVKLKKKDGSVFD
                     CLITASLWRDGDGKILGIQGILHDVTEQKRIEDELRQAHFELKQLLDSISSIVISVSA
                     RDVVTLWNPIAESILSLPAHLACGRRFSTLAIPWEWDRVSEGITASITGRKPVRIEDV
                     QLAAPDGSMRLLGIAINPITDAAGDPVGYLVRGADITERRSMEAHLAQAQKLEAIGQL
                     AAGVAHEINTPLQYINSNLSYIRDGINALTGHLGACGEIIDARDDSNTDRTLGALLGV
                     KERIARDVDLDEINKAVMESLDGVDRVVKIIRSMKELSHPGSEEMMPVDVNHAVENAV
                     TVSRNEWKYVADLLTDYDANVPPVPCYLNQLNQAVLNIIINAAQAIAESLLEPRADHA
                     DHV"
BASE COUNT         1327 a         1706 c         1953 g         1230 t
ORIGIN      
        1 atggcgaccg agaccatgga catcctgctc gtcgaagaca atccgggcga cgcgcgcctc
       61 atccaggaga tgctgaagga gggcaggctt gcgggcagcg aggtcgatgt tgtggagcgg
      121 ttggacgcgg cactacgccg cgccggggaa cggagatttg gcgccatcat tctggatctt
      181 aaccttccgg attccacggg ccttgatacg ctcgaccgca tcgtggccgc aaccggcggc
      241 atgatcccgg tgatcattct cacgggatta aacgacgagg tgatgggcga atcctccatc
      301 aatcgcggcg cggatgatta ccttgtcaag ggggacgtca cgctatcgca actggcgcgc
      361 tccctgcggt acgctgttga gcgcaagaga atggcgcggg aactcgccga gcgcgaggag
      421 cggtaccgtg ccctggtgga gcaccagggg gacgcggtgt gccgctggct tcccgatacc
      481 acgcttacct atgtgaacgg cgcctattgc gcgctcacgg gcgtgtcccg cgagcacgcg
      541 gcaggacgcc gatggatcga ttttgtaccc gccgaggacc gggaggcggt gctgtccttt
      601 tattgcgacc tccccgacgg tttggatgtc tatacctttg agcatcgggt gatgacaccc
      661 tcgggcgtca atcgctggat tttctggtcg gacgttccca tcaggtcggt caacgggaac
      721 atcgtcgagt ttcagtccgt tggccgtgac ataaccgagc gcaaagaggc cgaggatctg
      781 ctcacgagta attttaccct gctgcggatt gccggggaaa cggcgaagtt cggcggatgg
      841 cgggtggaca ttgccagcgg cattattttt tggtcggatg aggttgccgc cattcatgaa
      901 atgccgagcg gatattgccc ttccgtgaat gaggggattg acttttacgc gcctgaatgg
      961 agggacatga ttacgaggat ttttaccgat tgcgccgtaa agggcatacc ctatgacgag
     1021 gaaatggaaa tcatcaccgc gaaaggcaat cgggtgtggg tgagaacgat tggtgaggcg
     1081 gtacgggata caaatgggaa aataatacaa gttcagggat cgtttcagga tatcaccgag
     1141 cgcaaaagga tagaagaaaa aaccatggcc cagctcgaag agcttcgccg ctggcacgag
     1201 gccatgcttg accgtgagga gcgtgttatt gaactcaagc gcgaggtaaa cgatctactc
     1261 aagcgtttgg gcgaaccggc gcgctacagg agcgttgagg agtgaaaatg ggaggcggcg
     1321 gtctatgaag cggaacgaat accggaccat actggattcg ctgcccgtgg ggatcctggt
     1381 gctcgacgag caggtgtgcg tccgcgacgc gaacgatgcg gcgcaacgcc tgttcaacgc
     1441 cagcatcgac gaactgcggg accggcggtg cggcgacttt gtcgggtgcg ccaaccgcca
     1501 tgacaacgaa cgcggatgcg gctattcgtc cctgtgcggc tcatgcgccc tgttaaacgc
     1561 ggtaacggag gtgttcaccg gcgacaaggg agtctatgac cgggaggtgg aattcctgat
     1621 cgacagggac ggcgcgcccg tgcgggcgtg gatgcggttc agcgtcgtgc cggtcgtata
     1681 cgacggaagc cggtgcgtcg tcgtcgcgtt caatgatatc agcgaacgca aaagggccaa
     1741 ggaggccctt gagaaaagcg tggaaaaata tcgaaccctg acggaaaact cgcccgacct
     1801 catcgcccgt ttcgataggc agttgcggca ccgctacgtc aatgccgccg cagctgcggc
     1861 cggcacctta tcgcctgaag agtatatcgg taaaagcatc ggtgaaaccg gcgtcccgga
     1921 gcctgccaga tcgacgtggg aaaagcgaat ccaggccgtg tttgatgccg gaaatatgct
     1981 tgatgtcgtc gattcgttcc ccaccccggg cggcccgcgt cacttccata cgcgactggt
     2041 gccggaacgc gattcagcgg gaagcgtcca gtcggtcatc accattgccc gtgatataac
     2101 cgagcgtaaa ctggcggaag cgcaaaccat cgaggcccag gcggaaaccc aacgcctgct
     2161 ccgggaggcc gaccggtcgc ggagggcgct gctgagcgtt attgaggacc agaagatcgt
     2221 cgagaaaaaa ctggaggaaa cgctgtcggg gctggaacag cgcgtggccg agcgaaccgc
     2281 cgagctggtg gcggccaaca aggagctgga gtcgtattcg tacaccgtgt cacacgacct
     2341 gcgggcgccc ctgcgccata tcagcggttt tatcgatatg ctgcatcagc acatggggcc
     2401 ccgcctggat gttaagggcg cccattacat ggaagtgata gtcgattccg cccggtttat
     2461 ggggcagctc attgacgaac tgctcagctt ctcgcgaatg tcccgggctg atatggatgt
     2521 tggccgggtg gatctcaacc agctcgtcgg ttcgatcatc aaggaactga cggtcgaaaa
     2581 tcccgggccc gttccgacgt tcgagctggc caccctcccg gcagtgccgg gcgatgtcgc
     2641 gctgcttcgg atcgctttcg ttaatctctt gtcgaatgcg gtgaaattct cttcaacaaa
     2701 aaacgattcc cggatcacga tcggggccgt gtctcaggac gatacgacga tggtcgtcat
     2761 ccgggacaat ggcgtgggtt tcgacatgcg atacgcggat aagctcttcg gagtatttca
     2821 gcgactgcat gccgctgacg aatttccggg aaccggcatc ggcctggcga cggtgaagcg
     2881 aatcatcgag cgccatggcg gaagcgtccg ggccgagggg atgcccggcg agggggccgc
     2941 cttctatgtt tcaatcccaa gatgctcgca tgatgccgag gagggaaaat aatgaacgct
     3001 aaaacactcc tgctggtcga cgacagcgcg catgatgtgg aactcttcct cgcctcgtgc
     3061 ggtgaaagcg cgttacgcga acgtactgac gtcgctcggg acggcgcgga ggcgcttgac
     3121 tatctctatc gcaaaaacgc ctatcggtcc cggccgccgg ggaatccgta ttgcgtgttt
     3181 ctggacatta aattaccaaa ggttagcggc atcgaagttc tcgccattat gaagggggac
     3241 agcacgctca ggcacatccc ggtgatcatg ctctcctcgt cgagagaaga aagcgacatg
     3301 agcacgtgtt atcgttatgg cgcaaacggc tacatggtca aaccggtcat ggcgggcgat
     3361 ttggccccgg ctttgactgc ggcgtgtcat ttctggctgg atatcaacgt cgttccacat
     3421 cgtccggaca gcatgccggg aacgcctgtc gcggatggtg gtgcttcgtc gtcgatgagc
     3481 gatctgcgca tacttcacct cgaggacaat cctcgcgatt gtgaaatgat tcacgccgag
     3541 atggcgcgcg gcggtatctc ctcgcaaccg gtagtcgtgt cgaggaggga agattatgtc
     3601 aaggctctgt cgtcggtatc gccggacgtc atcctttccg attacctcct ccccggcatc
     3661 aatgggcttg aggcgctcaa gctggcgcag gagcatgcgc tcgatgttcc ctttatcttc
     3721 ataagcggcg ccatgggcga ggaggtcgcc gttgaaacgc tcaagctggg cgcgaccgac
     3781 tatgtcctca aggataagct gaaaaaactg gtcccggcca tccggcgggc gctgcaggag
     3841 tctggcgagc gcgcccggcg tgtcgcggcc gaggagcgtc tgtggggaat tctccacgag
     3901 cgcgaggtgc tgcttaagga gatccatcac cgggtcaaga acaatatgca ggtgatatcg
     3961 agcctcctga atatccagaa gcagcagccc gcgcgtcaat cgtttgacga gtgcatgcgc
     4021 tccatgcata tgaggatacg atccatggct ctcgtgcatg agcgcatcta tcacgcggag
     4081 acgcttgccg ccgttgattt cgcagggtgc gtacgggcga tcgtcgatga actcgtcaaa
     4141 tccgatgcgg tcgatgcgaa gcgaatcgac attgcctgca gcgtgccgcc gctgaccttg
     4201 ccggccgaca tcgccatacc cgcgggtttg atcgccaccg aattgatttc aaatgcgctc
     4261 cggcacgcct ttccggaaaa tcgcaatggc gctgtttcga tcaccatgct gacccagggt
     4321 aacgggtcga tcaagctttc cgtcgtggac aatggggtgg gtatcccgat gcggttcatt
     4381 cacggggggg agggtactct cgggctgcac ctggtggaga ttctcgttgg gcaaatcaac
     4441 ggaacgatcg agatttcacg ggatgaagga accgcggtgt ccgtcctttt tatgccgatg
     4501 ggaggatgaa tgataaaaaa aaagattctc atcgttgaag acgagagcat caacgtgatg
     4561 gttctcaaag gaatgctcac cggcatgggt catgaggttg tcggggtggc ggaaaccggg
     4621 agagaggcga ttgcgatggc ccggggcacc gttcccgacc ttgtgctgat ggacatattt
     4681 ctcaaggatg acgtggatgg cgttgaggcg gccagggaaa taaactcctt ctcgagcgtg
     4741 ccgatcatat acgtgacggc gtattccgac agggacacca tcgagcgggt gaaaacaacc
     4801 cggtcgtctg cgtgtatttt aaagccctat gaacttcagc agcttcaaac gaccatcgat
     4861 atcgcccttc accggtatac ggttgagaaa caactcaggg agagcgaaat gcgctaccgg
     4921 tcgctgttcg aatcctcgcg cgacgcggtg tacatccgta ccctggaggg gcggatcatt
     4981 gactgcaatg aggccatgct ctcgctcttc ggatacacca cggacgagat tatggatctt
     5041 gatgtcgcga ccgcgtatgt cgacgtcgcg gtaaaagaca gactgcagat cgcgatcacc
     5101 gcgcaggaag cggttcggga tttccccgtg aaactcaaga aaaaggacgg atccgttttc
     5161 gattgtctga taacggcgtc gctgtggagg gacggtgacg ggaaaatcct gggcatccag
     5221 gggattctgc atgacgttac cgagcagaaa cggatcgagg atgaattgcg tcaggcgcac
     5281 ttcgaactca aacagctcct cgattcgatc tcatccatcg ttatcagcgt ttcggcgcgc
     5341 gacgtcgtta cgctctggaa cccgatcgcc gaaagcatat tgtcgcttcc ggcgcatctc
     5401 gcgtgcgggc ggcggttctc gacgctggcg ataccctggg agtgggaccg cgtctccgag
     5461 ggcatcaccg cgagcatcac cgggaggaag ccggtcagga tcgaagacgt gcagcttgcc
     5521 gctccggatg gttcgatgcg gcttctggga atcgccatta atcccattac ggatgccgcc
     5581 ggcgatcccg tggggtatct cgtccgcgga gccgatatca cggagcgccg gtccatggag
     5641 gcgcatctcg cccaggcgca aaagctcgag gcgatcgggc agctcgcggc cggcgtcgcc
     5701 cacgaaatca acacgcccct gcagtacata aacagcaatc tttcatatat acgggacggc
     5761 atcaacgcgc tcaccggtca cctgggcgcg tgcggcgaga tcatcgacgc ccgcgacgat
     5821 tcgaataccg accgaacgct gggcgcgctc ctgggcgtca aggagcgcat tgcgcgtgat
     5881 gtcgatctcg acgaaatcaa caaggcggtc atggagtccc tcgacggggt ggaccgggtg
     5941 gtgaaaataa tccgatcgat gaaggagctt tcgcatccgg ggagcgagga gatgatgccg
     6001 gttgacgtca atcatgccgt cgagaacgcc gtgacggttt cgcgcaatga atggaaatac
     6061 gtcgccgatc tcctcaccga ttacgatgcg aacgtccccc ccgtcccctg ttacctgaac
     6121 cagctgaacc aggcggttct caatatcatc atcaacgccg cccaggccat agcggagtcg
     6181 ctgcttgagc cgcgcgccga ccacgcagac cacgtc
//
LOCUS       DAORHF010000218         6193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12712_length_6193_cov_4.369990, whole genome shotgun sequence.
ACCESSION   DAORHF010000218 DAORHF010000000
VERSION     DAORHF010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6193
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12712_length_6193_cov_4.369990"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="PKJ16_14795"
     CDS             <1..948
                     /locus_tag="PKJ16_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HNV48310.1"
                     /translation="GGAKGIAHIAVLEYLSSMGIPIHMLAGSSIGSVISALYACGSLT
                     RFRNDLLAMKKSDMISLFDPVFPKSGFIEGDRFIEFLSRYVPTTARLEDFSIPVNIVA
                     TEFFTGKTVVFDSGPVLDAVRASVSIPGVLIPVRRGQSLLVDGGVTNPLPIDVVEHMG
                     ADLTIAVNLHPTVVKTKFELLRNNSVNDAGGTETGRMNIKQAAPDNEPAADLSSDGEK
                     DRGIMRYFDKWFRTRESEKTEEKFPTIFEVIGQSIDIMEYMITEMTLQRHRPTVLIEP
                     NLIEMKTLDFLQARSALDEGTRACERQGKALLRKIKRRV"
     gene            966..1558
                     /locus_tag="PKJ16_14800"
                     /pseudo
     CDS             966..1558
                     /locus_tag="PKJ16_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465791.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            1676..2128
                     /locus_tag="PKJ16_14805"
     CDS             1676..2128
                     /locus_tag="PKJ16_14805"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HNV48311.1"
                     /translation="MRTLVSSLLTMVLLGDPLIAQSLRKCTITIIKKSGARVPLAVEV
                     ADTEPARQRGLMHRRSLDRGSGMLFVFSDVDYRSFWMKNTFIPLSIAYIDTDGVITDL
                     HDMRPLDTSVLYSSRYPSRYALEVNRGWFRDNDISIGARVLIDGCLGK"
     gene            2109..3239
                     /gene="hflX"
                     /locus_tag="PKJ16_14810"
     CDS             2109..3239
                     /gene="hflX"
                     /locus_tag="PKJ16_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HNV48312.1"
                     /translation="MDASVNRISPAADQRALLVAVQQSADDPEEVRDSLDELTLLVAT
                     AGAIVVETMVVKRASFDPAYLIGVGVLDRIKSINADCSIRLVVVDLNTIKPAQVRNLE
                     EYLGCRVVGRTEIILDIFARRARSAESKIQVELAQLRYILPRLKGLGGVLSRLGGGIG
                     TRGPGEKMLETDRRHIIRRISALKKKLDKISRHRQLVRKSRDRAFRGAVVGYTNAGKS
                     TLINRLAKDDLFVEDRLFATLDSFTRTVYINDDTTILLTDTVGFIRNLPANLIESFQS
                     TLEEIRHADFLVHVVDCTARSITGNRETVERELARLEAFDKPVLLFFNKADAATPEAI
                     ERIMIAHPSALVGSAATGSGIDALRSALQDLSERRRKFNRAG"
     gene            3282..4388
                     /locus_tag="PKJ16_14815"
     CDS             3282..4388
                     /locus_tag="PKJ16_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HNV48313.1"
                     /translation="MKHKGLYPLIALFAALAGAYLLFANATLSGNPFISKAQGLDDIE
                     KLEDLIIINGLEMSSNRPIDDSPLSVDGSIIYDDEFDAPSQRVKSSESTIASKKSSSP
                     RPAEIMAALKKEDARWRIHKHRIRKGENLWAIAHRYGVSHSLIIRMNEITDPDMLTAG
                     KQISVPSRRDFSNSAKRNVATRTAIAQKTPERHPSAYTGKRPGTRIDGEIARTASPVN
                     DLESDTKDRSSHPEAVRSDSPVRFSLQWPLRGKITSSFGKRTDPFTRKKAFHCGLDIS
                     ANVGTPIKAASSGRVIFSGWKDGYGKVVIMKHEHGYITVYAHNNKNFVSEGDSVDAGT
                     IIAESGMTGAVTGAHLHFELRKYLTPLNPVRFLR"
     gene            4385..5473
                     /locus_tag="PKJ16_14820"
     CDS             4385..5473
                     /locus_tag="PKJ16_14820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48314.1"
                     /translation="MKREVMHCSSTQRSVPRFIAASLLLLMIPVAALATIPIQSEARR
                     MKVFYAKTDTVGWDVVLRGTVQSIGTNIDIPPGDLSQTVQDKSKATIRFSDAFGIKPG
                     DTLYVVNENNLIIAKVIVRQVFKSQSFGHMAVGVGNLRLARASDRVVQKVDEEYSKYA
                     FIYKSRGDYFRHHGKIGEAIEQYKRAIEIDRRNPEAHLALGMIYLQKDLLQFAKKEFD
                     EGYREIRRMYDAEDRYHLLKGMVETRYRSLSYASVPLASRKTHKIEAIKYAKEALSLH
                     PSAKEMHYLIGLIYITKPEPMDVEAKNAFLKAIELDPELTDALVHLSVLYQRHNNNDK
                     ARLYANRALEVDPTNQRARYVLKSIESK"
     gene            complement(5495..5791)
                     /locus_tag="PKJ16_14825"
     CDS             complement(5495..5791)
                     /locus_tag="PKJ16_14825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48315.1"
                     /translation="MEYQIVESQDIVTIIANGPIEMRSIKDFKDKLLEMGDSTDKNIE
                     IDLSHVDYMDSTGIGLLITLLKKQKKKDKQLRIVNATPRVLSLLELSSLSEVLS"
     gene            5967..>6193
                     /locus_tag="PKJ16_14830"
     CDS             5967..>6193
                     /locus_tag="PKJ16_14830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48316.1"
                     /translation="MNREEKAKRNKKAILSAIKRCLDRVAYSYISMQDVADEAGISKG
                     GLRYYYPTKESLYMALIEDFFTEMDEDHLSI"
BASE COUNT         1513 a         1659 c         1658 g         1363 t
ORIGIN      
        1 ggtggggcga agggaatagc gcatatcgcg gtccttgaat atctttcatc gatgggaata
       61 ccgattcaca tgctggccgg atccagcatc ggttccgtca tatccgcatt atatgcctgc
      121 ggttccctga cgcgctttag aaacgatctt ctcgccatga aaaaatcgga catgatctcc
      181 cttttcgatc ccgtatttcc gaaatccggc ttcattgagg gggatcggtt catcgaattt
      241 ctgtcgcgct atgtccccac gaccgcccgt ctcgaggact tcagcatacc ggtgaacatc
      301 gtcgccactg agttctttac cggcaagacg gtcgtctttg attcaggccc ggtcctcgac
      361 gccgtgcggg caagcgtttc aattcccggc gtgctgatac cggttcgacg cggccagtcg
      421 ctgctggtcg acggcggggt tacgaacccg cttcccatcg atgtcgtcga gcacatgggc
      481 gcggatctca ccatcgccgt caatctgcac cccacggtcg tgaaaacgaa atttgaattg
      541 cttcgcaaca attccgtcaa tgacgcgggc ggaaccgaaa ccgggaggat gaacatcaaa
      601 caggccgctc cggacaacga gcccgccgcc gatctttcaa gtgacggcga aaaggatcgc
      661 gggataatgc gatatttcga taaatggttc cggacgcggg agtcggaaaa aaccgaagaa
      721 aaatttccca cgattttcga ggtgatcgga caatcgatcg acatcatgga atacatgatt
      781 accgagatga cccttcagcg ccatcgaccg accgtactta tcgagccaaa tctcatcgag
      841 atgaaaacgc tcgattttct tcaggcgcga tcggcgctcg atgaggggac gcgcgcctgc
      901 gaacgccagg gcaaggcatt gcttcgaaaa atcaagcgac gggtataata cgtctggagc
      961 gcggcatgtc acgaaaaggt gagctgcttg aatcgattcg cgtgtcaatg ggagagtgca
     1021 ggctgtgcaa gcttcacgcg acgcgcagca ccatcgtgtt cggcgagggg aaccccgacg
     1081 cccgcgtcat gttcatcggc gaagggccgg gacgcgagga ggacgcgcag ggcaggccct
     1141 tcgtgggtcg ggcggggcag cttctgacgg ccatcatcga gaagggcatg cggattcccc
     1201 gcagcgaggt ctatatcgcg aacgtcgtga agtgccgccc gacggtcgat cttgccatga
     1261 cgcgcgatcg gcctcccgac gaagaggaaa cgaacgcatg cagcggcttt ctccttcggc
     1321 aaatcgaagc gatcgcgccg gatgtgatca ttacactggg aaatccttca acgcggttcc
     1381 tgcttaaaac gacacttggc atcacaaaga ttcgcgggac ctggttcgaa tttaacggta
     1441 tacccgtcat gccgacctat cacccgtcta tgtgcttcgc aatggcggcg agaacagtcc
     1501 attgaaaaag gacgtttggg aggacattaa aaaggtgatc cttcgactcg ggttgcgcga
     1561 tgatatatcg gttcacaatg aacagcaggc agcaccgatc agcgatttcg aaagcctgag
     1621 ggatgataaa aaacccgatg acgaagttca gggcaccctg tttaaataat cagccatgag
     1681 aacgcttgta tcatcacttc tcacgatggt tttactgggc gatccgctta tcgcgcaatc
     1741 gcttcgaaaa tgcaccatca ccatcatcaa gaagagcggc gcacgagtgc cccttgcggt
     1801 tgaggttgcc gataccgaac cggcgcgcca gcgcggactg atgcaccgtc gatcgctcga
     1861 ccgcggatcc ggcatgctgt tcgtgttttc cgatgtcgac tatcgaagtt tctggatgaa
     1921 gaacacgttc ataccgcttt ccatcgccta tatcgacacg gacggcgtca tcaccgacct
     1981 tcacgatatg cgacccctcg acaccagcgt tctgtattca tcgcgatacc cgtcacgata
     2041 tgcccttgag gttaatcgcg gctggtttcg cgacaatgac atttccattg gcgcacgggt
     2101 gctgatcgat ggatgcctcg gtaaatagga tttctccagc agccgatcag cgtgcgctcc
     2161 tcgtcgcagt gcagcagagc gccgacgatc ccgaagaggt gcgcgattcg cttgacgagc
     2221 tcacgctcct tgtcgcgacc gccggcgcca ttgtcgtcga aacgatggtc gttaaacgag
     2281 caagcttcga tcctgcctac ctcatcggtg tcggcgtcct cgaccgcatc aagtcaatca
     2341 acgccgattg ctcgattcgg ctcgttgtcg tggatctcaa tacgattaaa ccggcgcagg
     2401 tgcgaaatct cgaggaatac ctcggctgtc gggtcgtcgg ccgcacggag atcattctgg
     2461 acatcttcgc gcgacgggcg cgcagcgccg aatcaaaaat acaggtcgag cttgcccagc
     2521 tccgatacat ccttccgcgc ctcaagggcc tgggcggcgt gctgtcccgt ctcgggggcg
     2581 gcatcggaac acgcggtccc ggcgaaaaaa tgctggaaac cgaccgccgc cacatcattc
     2641 gccgtatctc cgcattgaaa aagaaactcg ataaaatcag caggcatcgt cagcttgtca
     2701 gaaaatcgcg cgatcgagcg tttcggggcg cggtcgtggg atacaccaat gcgggaaaat
     2761 cgactctcat caaccgcctc gcaaaggacg atctgttcgt cgaggaccgt ttgttcgcga
     2821 cgctcgattc gttcacgcga acagtataca tcaatgacga tacgactatc ctgctcactg
     2881 ataccgtcgg ttttattcga aatcttccgg caaatctcat cgaatcgttt caatcaacac
     2941 tcgaggagat ccggcatgcc gattttctcg tccatgtcgt cgactgcact gcgcgaagca
     3001 taacgggcaa cagggaaaca gtagagcgtg aactcgcacg actcgaagcg tttgataagc
     3061 cggttctcct ttttttcaat aaggccgatg ccgccacccc tgaagcgatc gagcgaatca
     3121 tgatcgcgca tccctctgcg ttagtcggtt cggcggcaac cggttccggc attgatgctc
     3181 tccggtccgc actgcaggac ctttccgagc ggcgaaggaa attcaatcga gcggggtagt
     3241 catccctaaa gaatcgtcga tcgagtaccg atgtagtacg tatgaaacat aaaggtctct
     3301 atccgcttat cgcgctcttt gctgcgctcg ccggcgcata tcttctgttc gcgaatgcga
     3361 cactttccgg aaatccgttc atttcaaaag cgcaggggct tgatgatatc gaaaaactcg
     3421 aggacctgat cattattaac ggccttgaaa tgagctccaa ccgtccaatc gatgattccc
     3481 cgttgtccgt agatggatct attatatacg atgacgaatt tgatgcaccc tcccaacgtg
     3541 taaaatcatc cgaatcgacg atcgcgtcga aaaagtcatc gagtcctcgt ccggccgaaa
     3601 ttatggcggc gctgaaaaag gaagatgccc gatggcggat ccacaagcac cggatacgga
     3661 agggggaaaa cctctgggcg atcgcacatc ggtatggcgt cagccattcg ctcatcatca
     3721 ggatgaacga gattaccgac ccggacatgc tcaccgcggg aaaacagatc tccgtgccga
     3781 gtcggcgcga cttttcgaat tccgcgaaac ggaacgtggc gactcgtacc gccattgcac
     3841 aaaaaacgcc ggaacggcat ccaagcgcgt acacggggaa acgtcccggc acccgcatcg
     3901 atggagaaat tgcacggacg gcatcgccag ttaatgatct cgagtccgat acaaaggacc
     3961 gctcatcgca tccggaagcg gtccgttcgg acagtccggt tcgattttct ctccaatggc
     4021 cgcttcgggg aaaaatcaca tcatcgtttg gcaaacgaac cgatccgttt acccggaaaa
     4081 aggcgttcca ttgcgggctc gatatcagcg cgaatgtcgg tacgcccatc aaggccgctt
     4141 cctccggacg cgtcattttc agcggatgga aagacggcta tggcaaggtc gttatcatga
     4201 agcatgaaca tggctacatt acggtatatg cgcacaataa caagaatttc gtctccgagg
     4261 gcgatagtgt cgatgcggga actatcatcg cagaatcggg aatgaccggc gccgtcaccg
     4321 gtgcgcatct ccattttgaa ttgcgaaaat atctcacccc gttaaatccc gtgcggttct
     4381 tgcgatgaag agagaagtca tgcattgttc ttcaacacaa cgaagtgttc ctcgttttat
     4441 tgcagcatcc ttattattgc ttatgattcc tgtcgctgcg ctcgctacga ttccgattca
     4501 atcggaagca cggcggatga aggtgtttta cgcgaaaacc gatacagtcg gctgggacgt
     4561 ggtgctgcgc ggcacggtcc agtcgatcgg aacaaatatc gacattcccc ccggggactt
     4621 gtcccaaacc gtgcaggaca aatcgaaggc gaccattcgt ttcagcgatg cgttcggcat
     4681 caagccgggc gatacgctct acgtggtcaa tgagaacaat cttatcatcg ccaaggtgat
     4741 tgtccgccag gtgttcaaga gccaatcgtt cgggcacatg gctgttggcg tgggcaacct
     4801 tcggctggcc agggcaagcg accgcgttgt gcagaaggtt gatgaggaat attccaaata
     4861 tgcgttcatt tacaaatccc gcggggatta cttccgccac catggcaaga tcggagaggc
     4921 gatcgaacaa tacaagcggg ccattgagat tgaccgccgg aacccggaag cgcacctggc
     4981 gctgggaatg atctacctgc aaaaggacct cctgcaattt gcgaagaagg agttcgacga
     5041 gggatatcgc gagatcagga gaatgtacga tgcggaggat cgctatcact tgctgaaagg
     5101 catggtcgaa acccgatatc gcagtttgag ttatgcgagc gttccgcttg cgtctcgaaa
     5161 gacccataag attgaagcga tcaagtacgc gaaagaagcg ctctcgttgc atccgagcgc
     5221 gaaagagatg cattatctca tcgggctgat atacatcacg aagccggaac cgatggatgt
     5281 cgaggcgaaa aacgcctttt taaaggcaat tgagctcgat ccggagctca ccgatgcgct
     5341 cgtacatctc tccgtgctct atcagcgcca taacaacaac gacaaggcgc gactgtacgc
     5401 gaatcgggcg ctcgaggttg acccgacgaa ccagcgggcg cgttatgtgt tgaaaagcat
     5461 cgagagcaaa taggctcggg acggcatcac agtgctagga gagcacctct gaaagcgaac
     5521 tgagctcaag caggctgagc acgcgcggcg ttgcattgac gatgcgaagt tgtttgtcct
     5581 tctttttttg tttcttgagc agggttatca gaagtccgat tccggtcgaa tccatataat
     5641 cgacgtggga aaggtcgatc tcgatattct tgtcggtgga atcacccatt tcaaggagct
     5701 tgtccttgaa atcctttatt gaccgcatct ctatcgggcc gttggcgatg atggtgacga
     5761 tatcctgcga ctcgacaatt tgatattcca ttaatagatc ctacaaaatt caaatttgat
     5821 tttgaaaata tattcgcatt actttttgat atttttgtca agataaaatc aagcgaagcc
     5881 tgaaaaaatg gcccgtaaat atttcttgac caaattttaa tgtttttaca taaatagctt
     5941 tcatcacaat ttgcaacagg aacagaatga atcgcgaaga aaaggcgaaa cggaacaaga
     6001 aggcgatcct cagcgctatc aagcggtgcc tggatcgggt cgcgtattcg tatatctcga
     6061 tgcaggacgt cgccgacgaa gcgggaatat cgaagggcgg gcttaggtat tattacccca
     6121 cgaaggaatc cctatacatg gccctcatcg aggatttttt caccgagatg gacgaggacc
     6181 atctgtcgat aat
//
LOCUS       DAORHF010000219         6150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12840_length_6150_cov_5.288269, whole genome shotgun sequence.
ACCESSION   DAORHF010000219 DAORHF010000000
VERSION     DAORHF010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12840_length_6150_cov_5.288269"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..2449)
                     /locus_tag="PKJ16_14835"
     CDS             complement(89..2449)
                     /locus_tag="PKJ16_14835"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HNV48317.1"
                     /translation="MRRRIAVMIAACAVACAAAVFAERPDPTREGERRCESCYRMHPE
                     FRTVDGYGRGALGAAISSNATCGACHDAAFINRHNTHYTDAVKADCAECHFAGGRLGF
                     ERAAAAGGGMLRTGAIRLQPPRGENCGRCHGVVHEGTAPLAVPDDYGTRSRYDGRGAH
                     YGITQNTGAILSGQYLSQSHLNLKGKSGLNIPWDNHAQRGVACSSCHYAANDPRRCAV
                     IRSDIDYLKTDPRKVQSLGKYLHRPDHQLVASTCTSCHNPYAAHKRLPFKKKHFTALD
                     CQSCHVPRVYGPAYAAVDETVVRANGAPRVEYRGMDPTDESPVNARYAQGYVPYLFTR
                     PSSKAGDVVSPYNLVTTWYWRSADGKRVPAELVRRAFLDKGTYRSPVIALLDANHDKT
                     LQTTELVLDTPEKVAAIRRSLEELGVRGPEIAGEIAAHRINHGIVSGKSMTLDCAFCH
                     SDRSSLAHDVLLTRYSPDGALPRLVGDAPPRINGYVSREDDGRVIVDRRRQTEGRYIF
                     GFSRLWWIDTLGFTVFMVVAFGVGVHALLRYYTTRYHPTPLEKTTRLFVYTFYERVWH
                     WVMAVSVIILAVTGLEIHYAGSTGLLGFANAVSVHNALALVVAANAGLSLFYHLATGR
                     IRQFFSVDRSFGTMVLAQASYYLYGIFNQWPHPVEKTPDRTMNPLQQAAYVFLLNVLF
                     PLQIITGVLMWLSGLTVIGPDALTVIAPLHSLGSWFFISFLLVHVYLTTTGRRITSYL
                     EGMITGYEEVDERTHAGELRTVTRREIVDTMKHIFRRHREEADHER"
     gene            complement(2446..3915)
                     /locus_tag="PKJ16_14840"
     CDS             complement(2446..3915)
                     /locus_tag="PKJ16_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HNV48318.1"
                     /translation="MKKPSQPIVLIAIVAFIVMVPAIVSLVRFQARAHDPWSSITRSR
                     PHQDHSGYFTAEFSNPQEVTRSCLSCHPKAAKDVMKTAHWNWEGHEVTLPGRSGPMKI
                     GKKNLINNFCIGIRGNERSCTSCHVGYGWTDATFDFTKEENVDCLICHDWTGTYQKGD
                     GAMPKTGVSLLAAAKGVGYPRRENCGTCHNYGGGGMGVKHGDLDQTLVYAAEDVDVHM
                     GKHNLLCIDCHRTERHDIKGRAFSVGVDHVNGIACADCHINVPHRDRRIDAHLDAVSC
                     EACHIPSYARRAPTKVSWDWSKAGDSSRPDDIHRYLKKKGEFVYATNVVPEYLWFNYT
                     VDRYLLGDVINPDAVTRINPPRGSLADPRAKIWPFKVHRATQIYDRAFRYLLQPVTAG
                     EGGFWSEFNWDKAARLGEKVTGLAYSGTYGWARTEMHWPISHMVAPARDALKCADCHG
                     ATATRMNWKALGYPDDPADVGGRKRLAGARPAARREVAK"
     gene            4472..4897
                     /locus_tag="PKJ16_14845"
     CDS             4472..4897
                     /locus_tag="PKJ16_14845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV48319.1"
                     /translation="MRTTTDIKVRGYHLDLFSHVNNARYIELLEEARWDYIEKNSIQL
                     DEMFARGIAFNVVNITINYRRPGKLGDVLAIDTFVSRVGNKSMTMTQIITLKGTETRV
                     ADAEVTFVFAYMNEGKAIPIGDDIKRHWNWDDDLAGLPA"
     gene            complement(5018..6073)
                     /locus_tag="PKJ16_14850"
     CDS             complement(5018..6073)
                     /locus_tag="PKJ16_14850"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HNV48320.1"
                     /translation="MSEFFFNLTPDVVLSAIERLGFEPTGHCMALNSYENRVYDLRLE
                     DGAHVVAKFYRPGRWSRAQIQEEHDFLLELQADEIPVCAPLSTPDGATIHEIEGIFYT
                     VWPRTGGRVPDELSDEVLGMLGRLCARIHNNGAAKRADHRISLNANTYAREPLAFLEA
                     GDFLPPHCRDRYRAAVDEVAAIYEALVADVPLHRIHGDCHLGNLLHGNEGWFFLDFDD
                     FVTGPAVQDVWMLVPARDEEGLRQREIFLSAYRQFRDFDSSWLRLVEPLRALRFIRYA
                     AWIAKRWDDPAFPAAFPHFGTVQYWEDETADLEKQLEVIHGATHELPESVRRDDEPAE
                     EQKELTNKDYFWDWEEK"
BASE COUNT         1137 a         1946 c         1970 g         1097 t
ORIGIN      
        1 gctctgggtg aaaaaatagg cggagaagag gaccacgccc agcaggatcc cgccgacata
       61 gggattgatg tagccgcgcg cccccgaatc accgttcatg gtcggcctcc tcgcgatgac
      121 gtctgaagat atgcttcatg gtgtcgacga tctcgcgccg cgttacggtg cgcagctcgc
      181 ctgcgtgcgt ccgctcgtcg acctcctcgt agccggtgat catgccctcg agatagctgg
      241 tgatgcggcg gcccgtggtg gtgagataca catgaaccag cagaaacgaa atgaagaacc
      301 acgagccgag gctgtgcagc ggcgcgatca ccgtgagcgc gtcgggaccg atgacggtga
      361 ggcccgacag ccacatgagg acgccggtta ttatctggag cgggaagagc acgttaagca
      421 agaacacata cgccgcctgc tggagcggat tcatggtccg atccggcgtc ttctcgaccg
      481 gatgcggcca ctggttgaag atgccgtaca gataatacga cgcctgcgcc agcaccatcg
      541 ttccgaagct ccgatcgaca ctgaagaact ggcgaatccg ccccgtggcg agatgataga
      601 acagcgagag gcccgcattg gcggccacca caagggcgag cgcattgtgc acgctcacgg
      661 cattggcgaa cccgaggaga ccggtcgaac cggcgtagtg gatctcgagc ccggtcacgg
      721 ccaggatgat caccgacacc gccatcaccc agtgccacac gcgctcgtag aacgtgtaca
      781 cgaacaggcg cgtggtcttc tcgagcgggg tcggatgata cctcgtcgtg tagtaccgca
      841 gcagggcgtg gacgccgacg ccaaacgcca cgaccatgaa cacggtaaag cccagcgtgt
      901 cgatccacca gaggcggctg aagccgaaga tgtagcgccc ctcggtctgc cggcgccggt
      961 cgacgatcac gcgcccgtcg tcctcgcgcg acacgtatcc gttgatgcgc ggcggcgcgt
     1021 ccccgacgag gcgcgggagg gcgccatccg gcgagtaccg ggtgagcagc acgtcgtggg
     1081 cgaggctcga acgatcgctg tggcagaaag cgcagtcgag cgtcatcgac ttcccgctca
     1141 cgatgccgtg gttgatccgg tgcgccgcga tctctccggc gatctcggga ccgcggacgc
     1201 ccagctcctc gaggctgcgc cgaatggcgg cgaccttctc cggcgtatcg aggaccagct
     1261 cggtcgtctg cagtgtttta tcgtggtttg catcgaggag cgcaatgacc ggactgcggt
     1321 atgttccctt gtcgaggaat gcccgacgca ccagttccgc gggtacgcgc ttcccgtccg
     1381 ccgaacgcca ataccaggtg gtcacgagat tatacggcga cacgacatcc cccgccttcg
     1441 acgagggccg cgtgaacagg tacggcacat atccctgcgc gtagcgcgca ttgacgggcg
     1501 attcgtccgt cgggtccatc ccgcgatact ccacgcgcgg cgcgccgttc gcccgcacca
     1561 cggtctcgtc gaccgctgca tacgcagggc cgtacacccg cgggacatgg caggactggc
     1621 aatccaacgc cgtgaagtgc tttttcttga acgggagccg cttgtgcgct gcgtagggat
     1681 tgtggcacga cgtgcacgtc gacgccacga gctgatggtc gggacgatgg aggtatttcc
     1741 ccagtgactg caccttgcgc ggatcggtct tgaggtaatc gatgtcggag cggatcaccg
     1801 cgcagcggcg cgggtcgttc gccgcatagt ggcacgacga gcaggcgacg ccccgctggg
     1861 cgtgattgtc ccatgggata ttgagcccgc tcttcccttt cagattcagg tgcgactgag
     1921 acaggtactg gccggagagg atcgcgccgg tattctgggt gatgccgtag tgcgcgccgc
     1981 gtccgtcgta gcgcgacctc gtcccgtaat cgtccggcac tgcgagcggc gcggtcccct
     2041 cgtgcaccac gccgtggcag cggccgcagt tctcgccgcg cggcggctgt aggcggatgg
     2101 cgcccgtgcg caacatcccg ccgcccgccg cggcggcgcg ctcgaacccg aggcgcccgc
     2161 cggcgaagtg gcattcggcg caatcggcct tcaccgcgtc ggtgtagtgc gtgttatggc
     2221 ggttgatgaa cgcggcatcg tggcaggccc cgcacgtcgc gttggacgat atcgccgcgc
     2281 cgagcgcgcc gcgcccgtac ccgtcgaccg tgcggaactc ggggtgcatg cgataacagg
     2341 actcgcagcg ccgctccccc tcgcgcgtcg gatcgggacg ctcggcgaat acggcggcgg
     2401 cgcaggcgac ggcgcacgcc gcgatcatca cggcgatgcg gcgcctcatt tcgcgacctc
     2461 ccgccgggcg gcaggccgcg cgcccgcgag ccgctttctc ccgccgacgt cggccggatc
     2521 gtcgggatag ccgagcgcct tccagttcat gcgggtcgcc gtggcgccgt ggcaatcggc
     2581 gcacttcaac gcatcgcgcg ccggggccac catgtgcgag atgggccagt gcatctcggt
     2641 gcgcgcccag ccgtatgttc cgctgtacgc gagccccgtg accttctcgc ccagacgcgc
     2701 ggccttgtcc cagttgaact ccgaccagaa cccgccctcg cccgccgtga ccggctgcag
     2761 gagatagcgg aacgcgcgat catagatctg cgtggcgcgg tggaccttga acggccatat
     2821 cttcgcccgc gggtccgcga gggaaccgcg cgggggattg atccgcgtca cggcgtcggg
     2881 gttgatgaca tcgcccagaa ggtaccggtc caccgtgtaa ttgaaccaga ggtattccgg
     2941 gaccacgttg gtcgcgtaca cgaactcccc cttcttcttg agataacggt gtatgtcgtc
     3001 cggacgagag gaatcgcccg ccttggacca gtcccagctc accttggtgg gagcgcgccg
     3061 cgcataggac gggatgtggc aggcctcgca ggagacggcg tcgaggtgcg catcgatgcg
     3121 gcgatcgcgg tgcggaacat tgatgtggca atcggcgcag gcgatgccgt tcacgtgatc
     3181 gacccccacc gaaaaggcgc gccccttgat gtcgtgccgc tcggtccgat ggcagtcgat
     3241 gcacaagagg ttgtgctttc ccatgtggac atcgacatcc tccgcggcgt acaccagcgt
     3301 ctggtcgaga tcgccgtgct tcacgcccat cccgccgccg ccgtagttgt ggcaggtgcc
     3361 gcaattttcg cgccggggat agccgacgcc cttcgccgcc gcgagcaggc tcacgcccgt
     3421 cttgggcatc gcgccgtcgc ccttctgata ggtcccggtc cagtcgtggc agatgaggca
     3481 atcgacgttt tcttccttgg tgaaatcgaa ggtggcgtcg gtccacccgt agccgacatg
     3541 gcaggaggtg caggagcgct cgttcccgcg aatcccgatg cagaaattgt tgatgaggtt
     3601 cttcttgccg attttcatcg gtccgcttcg accgggcagc gtcacctcat gcccctccca
     3661 gttccagtgg gcggtcttca tcacatcttt ggcggccttc gggtggcagg agaggcatga
     3721 tctcgtgacc tcctgcggat tggaaaactc ggcggtgaaa taccccgaat ggtcctgatg
     3781 gggacgcgag cgcgtgatcg acgaccacgg gtcatgcgca cgcgcctgga agcggaccag
     3841 ggaaacgatc gcaggcacca tcacgatgaa ggcgacgatg gcgatgagca ctatcggttg
     3901 gcttggcttt ttcatgtgtt accggcaccc gctacaaaac gagttgcatc ggcatctcag
     3961 aaaaccgcat cgctgtcaat gatatttctc caccgcaagg cctatcaaaa cacagcggac
     4021 aggatcgctc ctgcccgcgt aacatctttt acatcatgtt gatagctaaa tccaggctaa
     4081 tcgctcaaga tatgcatcga actcttcccg ggaaaaacgc actctcgtgc tgcattcgtg
     4141 ggtcaggcac atatcatata tcttgttcat cgccgtatcc tccttagtga gactaatgca
     4201 atgtttatat tttaatataa tattaaatac taaatatgtc aagctctttt cttgcgattg
     4261 tggtaaaatt tccatatgct tcgcatgcgc atgaccgtcc gggctcgacc gcggaagaca
     4321 ccaagagcgc gagaggagca cgagcgaacc tgcgccatcc acgcggcggg atgcacggcc
     4381 atctcgatca ccggagggca tcgcgcggaa tcgcattgac aaaaccgccc gccgactgga
     4441 gggtgatcga tatcgccctc caggagcagc catgagaacg acgaccgaca tcaaggtgcg
     4501 cggctaccat ctcgacctct tctcgcatgt gaacaatgcc cgctacatcg agctgctcga
     4561 ggaggcgcga tgggactaca tcgaaaagaa cagcatccag ctcgacgaga tgttcgcgcg
     4621 ggggatcgcc ttcaacgtgg tgaacatcac catcaactac cggcgcccgg ggaagctcgg
     4681 cgacgtcctc gccatcgaca ccttcgtaag ccgcgtggga aacaagagca tgaccatgac
     4741 gcagatcatc acgctcaagg ggaccgagac ccgcgtcgcc gacgccgagg tgaccttcgt
     4801 gttcgcctac atgaacgagg gcaaggcgat cccgatcggg gacgatatca agcgccactg
     4861 gaactgggac gacgatctcg cggggctgcc ggcataagcc ccccctccgc tctccgggca
     4921 cctccccctt cgttaggggg aggtgttata atcacccatt gttgtgcaat cggcgtgtga
     4981 cgctggttcg ccattttttt cactgcgggc aggcgggtta tttttcttcc cagtcccaga
     5041 agtagtcctt gttagtgagc tccttctgct cctcggcggg ttcgtcgtcg cggcgcacgc
     5101 tctcgggcaa ttcgtgggtc gcgccatgaa tgacctcgag ctgcttctcg aggtcggcag
     5161 tctcgtcctc ccagtactgc accgtgccga agtgcgggaa cgccgccggg aaggcgggat
     5221 cgtcccagcg cttcgcgatc catgcggcgt agcggatgaa gcgcagggcg cgcagcggct
     5281 ccacgagccg gagccacgac gaatcgaaat cgcggaattg ccggtaggcc gagaggaaga
     5341 tctcgcgttg gcgcaggccc tcctcgtcgc gcgcgggaac gagcatccac acgtcctgaa
     5401 ccgccggtcc cgtcacgaaa tcgtcgaaat ccagaaagaa ccagccctca ttcccgtgca
     5461 ggagatttcc caggtggcag tcgccgtgga tgcgatggag cggcacgtcc gcgacgagcg
     5521 cctcgtagat cgccgcgacc tcgtcgacgg ccgcgcggta gcggtcgcgg caatggggcg
     5581 ggaggaaatc gcccgcctcc aggaaggcga gcggctcgcg ggcgtaggtg ttcgcgttaa
     5641 gcgaaattcg atgatccgcc cgcttcgccg cgccgttgtt gtggatgcgc gcgcagagcc
     5701 gcccgagcat gcccagcacc tcgtccgaga gctcgtcggg cacgcggccg ccggtgcggg
     5761 gccacacggt gtagaagatg ccctcgatct cgtggatggt cgcgccgtcg ggcgtcgaaa
     5821 gcggcgcgca cacggggatc tcgtcggcct ggagctcgag gaggaaatcg tgctcctcct
     5881 ggatctgcgc gcggctccag cggccggggc ggtagaactt cgccaccaca tgcgcgccgt
     5941 cctcgagccg caggtcgtag acgcggttct cgtagctgtt gagcgccatg cagtggccgg
     6001 tgggctcgaa gccgagccgt tcgatcgctg acaggacaac atccggcgtc aggttaaaga
     6061 agaactcgct catgtcaccc gttcatgcgc gcgatgtgcg cgacggcggt gaaatgcagc
     6121 agcgcgccgg cgaggataaa gagatgaaac
//
LOCUS       DAORHF010000220         6105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_12989_length_6105_cov_4.365785, whole genome shotgun sequence.
ACCESSION   DAORHF010000220 DAORHF010000000
VERSION     DAORHF010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12989_length_6105_cov_4.365785"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="PKJ16_14855"
     CDS             <1..1725
                     /locus_tag="PKJ16_14855"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV48321.1"
                     /translation="RMFAKWSRHAPQNHQHKLDLISAEIARIKGDYRTAEKLYDRAIA
                     GAKENAYVHEEAIACERAANFYSERGLQYNAANYMKMAHARYTTWGAMAKVKQLEEKY
                     PEYLQEVWSGHALTIPTREDDSSTSSSSVASSLDIGTVLKASRAISGEIDLPRLLETM
                     LRIVMENAGADRGALIREKDSALIVEAEGSVENDIIVESHELTSTERMTFPPPNEGGG
                     PGRRLFPVSIINYVHRTGETVLLDDAANRELFSGDPYVTVQKSKSILCAPIREKGKYS
                     GILYLENNLVAGAFTPERLELLNIISSQAAISLENARLVAMEKEKAALDREIEMAEKI
                     QRSLLPREIPEIVGARVAFRYVPMTGVGGDFVAIRHDAARGRLGLFICDVSGHGVPAA
                     MTASMVSAALDFHWSGMPDDLPGVFRGMHAFLKGKMGGNFFTACLCTLDLDTGTLVLS
                     SAGHPAAVVVRADGAAEMADVKGRLINEFFEPKLSVVTVRLSPGDRVVLYTDGITEAM
                     NPSGAMLGADDDEFCRLVARIADESSSPDDLCDRIHRRVIEHAGTTSLQDDVTVLVCE
                     YAGIRHSV"
     gene            1900..3759
                     /locus_tag="PKJ16_14860"
     CDS             1900..3759
                     /locus_tag="PKJ16_14860"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV48322.1"
                     /translation="MVLFLASLFLVSCSVESSIDVSRGWRFTTGDDQSYKNIDCDDSR
                     WFPTDLPARLSPDKKRYVFWLRKKAVVPDEYRGREIAISLGKIWDADETYFNGVKIGE
                     SGSEPPNWQSMWNYDRYYHIPSPFIRYGDVNVIAVRMYTNTLPLFNGRPFIAVLEQVR
                     VHNFWKRFAAEYLVVAFTVISLFLSLVSLVQYFSFRRDRVSLLYSIITFILTIASFQY
                     YLPASPMVSATVKDIVYYTLMTTAGYLSLFLFEMFSGRRHRVVRVIMLVISPVSYALI
                     LTSSLDDPLQGWRFDIIGMILNITLLLLGYIVTSSVIRERSRESIAIVIGYLVLILCC
                     LNDIFFMMNLYYSDFFLIPYGWVACYITIGIALAFRTSRTMYRNWDIENHMEIARLIQ
                     QKLLPQSLPDTPGYRAHAAYVPMDKVGGDFYDITRYGDTIRVFIADVSGHGLASSYLA
                     MIARMSLESVSGNRSTGAVIKHINEAICRSTVKNNYVTSFYAEIDLSTNVIRYTNAGQ
                     FPPLVYRAGTGEFFELMTRGRPLGWFLSLLVEEKSFQLSSGDRLILFTDGIIECANRK
                     GEMFGEERFREFIRSHGQLHTGEFADRLLSLLKEHRGTGSFDDDVTLIVLDVL"
     gene            complement(3812..4495)
                     /locus_tag="PKJ16_14865"
     CDS             complement(3812..4495)
                     /locus_tag="PKJ16_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HNV48323.1"
                     /translation="MPKPPLRIQTTTLWDYPSQNYGNAPQGDQNYVGATPSYIIWNLL
                     QRYTKPGDLVVDPMCGSGTTIDVCKDLERRFLGYDINPSRKDIFRADARKLPLENEKA
                     DFVFVDPPYGDNIDYSNEKKCIGKLPATDERYFEAMRAAIGEIHRILRRKRAMALYVS
                     DYYNQKHGFVPIGFRLFGMLSEQFTPMDIISVTRHNKDFKKGNYHKAAEEGNFFLRGF
                     NYLFVMWKG"
     gene            4619..5551
                     /locus_tag="PKJ16_14870"
     CDS             4619..5551
                     /locus_tag="PKJ16_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48324.1"
                     /translation="MDNLFIYISKQLKFEKFPRCFLLTWVFIVTGLMVFVLFQFLAGL
                     MPRAGFFALFLQFMGIFLLFTSLLMAYAGVYAITTAPQDVAGITRAAIVATVRRSYMI
                     LGVSFLTLFVYVAFLLLQVGVSSIAIIPYVGPVVIALFTAPLLLVNLAGLIAVIIVFA
                     VFPPLASEVRTMRDLFLEFKISVQGRWLKVALYTLVSFALLVLCVLCIYYLLMYVAGI
                     SRAVQGSIITAYPSLMDTLAMKSAVSEAIRELAPRGASIASMREARILFTDLHGILSF
                     LVGLSYLVLLSAVMAFPLAAYFNLSSIFFGRVKR"
     gene            5574..>6105
                     /locus_tag="PKJ16_14875"
     CDS             5574..>6105
                     /locus_tag="PKJ16_14875"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48325.1"
                     /translation="MMPRTVVGIVLICAAVVAPHALHGAECAEGDCANGVGRFRFDDG
                     SEYAGSWQGGRFHGEGTMCYADGAEYRGAWRSGKRHGRGTHIAPDGSEYCGTFADDRP
                     AGEGVYLGIDGRRRRVTIRDNKMVPTLRLENEKPEDGCQYGEVDYKGRYHGWYRGRKF
                     DAFKPHGFGAMTWPNGA"
BASE COUNT         1119 a         1747 c         1863 g         1376 t
ORIGIN      
        1 cgaatgttcg cgaaatggtc tcgtcatgca ccacaaaacc atcagcacaa gctcgatctc
       61 atatctgccg agatcgcacg aatcaagggc gattatcgta cggccgagaa gctgtacgat
      121 agggccattg ccggcgcgaa agaaaatgcg tatgtccatg aggaggccat cgcctgcgag
      181 cgcgctgcta atttctattc ggaacgcgga ttgcagtata atgcagcgaa ttacatgaag
      241 atggcgcatg cgcgctacac gacgtggggc gcgatggcga aggtgaagca actggaggag
      301 aagtatcccg agtacctgca ggaagtatgg tcagggcatg ccctgaccat accaacacgc
      361 gaggacgact cgtccacctc gtcctccagc gtcgcgtcgt ccctggacat tggcaccgtg
      421 ctcaaggcgt cgcgcgccat ctcaggtgag atcgaccttc cgcgcctcct ggagacgatg
      481 cttcgcatcg tcatggagaa cgccggtgcg gatcgcggtg ccctcattcg cgagaaagac
      541 agcgcgctca tcgtcgaggc ggagggctcc gtcgagaatg acattattgt ggaaagccat
      601 gaactcacct ctaccgagcg aatgacattc cctcccccta acgaaggggg agggccgggg
      661 aggaggttgt tcccggtctc catcatcaac tatgtgcacc gtacgggcga gacggtcctc
      721 ctcgacgacg cggcgaacag ggagcttttc agtggcgatc cctacgtgac ggtgcaaaaa
      781 tcaaaatcga tcctctgcgc tcccattcgc gagaagggca aatactcagg aatcctctac
      841 ctggaaaata atctcgtcgc cggggcgttc accccggagc gcttggaact tttaaacatc
      901 atctcgtccc aggcggccat ctctcttgag aacgcgcgtc tggttgccat ggaaaaagaa
      961 aaggctgccc tcgatcgcga gatcgagatg gcggagaaga tccagcgctc gctgttgccc
     1021 cgggagatcc cggagatcgt tggggcgcgc gtggccttcc ggtacgttcc catgaccggg
     1081 gtggggggcg atttcgtcgc gatccgccac gatgccgcgc gagggaggtt gggcctgttc
     1141 atctgcgacg tgtcgggtca cggcgtgcca gcagccatga cggcctcgat ggtgagcgcg
     1201 gcgctggact tccactggtc cggcatgccc gatgacctcc cgggcgtctt ccgcgggatg
     1261 cacgcctttc tcaagggaaa gatgggaggg aatttcttca cggcgtgcct gtgcaccctc
     1321 gatctggata cggggacgct ggtcctgtcg agcgccgggc atccggccgc ggtcgtggtg
     1381 cgcgcggacg gtgcggctga aatggctgac gtgaagggca ggctcatcaa cgagttcttc
     1441 gaaccaaagc tttcggtcgt gacggtgcgt ctttctccag gcgatcgggt ggtgctctac
     1501 accgacggga tcaccgaggc catgaatccg tcaggcgcga tgctgggcgc cgacgatgac
     1561 gagttctgcc gtctagtggc gcggatcgcg gacgaatcat catcgccgga cgacctgtgc
     1621 gatcgaatac accgccgggt gatcgagcac gccggtacga ccagcctgca ggacgacgtg
     1681 acagtgcttg tgtgcgagta tgcggggatt agacattctg tttgattttt cctgcgatca
     1741 aagataccat gtaaatatcg atatatttgc gtgcgttgtt gtacatgcga gccaagagct
     1801 ggaatggcaa cagatgtgaa atcctgcatg aaatcgtatt gggctggcaa ttcaaggatg
     1861 cgcgcgtggc atgggcgtcg aagattcata tcgttgttga tggtgttgtt tctcgcttct
     1921 ctgttcctcg tctcgtgttc cgtcgagagc agtatcgacg tgtcgagggg gtggcgattc
     1981 actacgggcg acgaccagtc gtataaaaac atcgattgtg acgattcccg atggttcccg
     2041 accgacctgc ccgcgagact atccccggat aaaaagcggt acgtcttctg gctcaggaaa
     2101 aaggccgtcg ttccggatga ataccgcggc agggagattg cgatctccct cggcaagata
     2161 tgggatgccg acgagaccta cttcaacggc gtgaagatcg gcgaatcggg ttccgagccg
     2221 ccgaactggc agtcgatgtg gaactatgac cgttactatc acattccatc gccgtttatt
     2281 cggtatgggg acgtgaacgt gatcgcggtc aggatgtata ccaacacgct cccgcttttc
     2341 aacgggaggc cgttcatcgc ggtgttggag caggtccggg tgcataactt ctggaaacgc
     2401 ttcgcggccg aatatctcgt cgtcgccttc accgtcatca gccttttcct gtcgcttgtg
     2461 tcgctggtgc agtatttcag cttccggcgc gaccgggtat cgttgctgta ttcgattatt
     2521 accttcatcc ttacgatcgc ttcattccaa tattatctgc cggcatcgcc gatggtttcc
     2581 gccaccgtca aggacatcgt ctattacacg ctgatgacca cggctggtta cctgtccctg
     2641 ttcctgttcg aaatgttctc tgggaggagg caccgggtcg tccgtgttat catgctggtg
     2701 atctcgccgg tctcctatgc gctcatcctg acctcatcgc tcgacgatcc cttgcaggga
     2761 tggcgtttcg acattatcgg catgatcttg aatatcaccc tcttgctgct cgggtacatc
     2821 gttacctctt cggtcatacg cgagcgctcc agggaatcta ttgcgatcgt gatcgggtac
     2881 ctggtcctga tcctgtgctg cctcaatgat atcttcttca tgatgaacct gtactactcg
     2941 gatttcttcc tgatcccgta tggatgggtg gcatgttata tcacgatcgg catcgcgctc
     3001 gccttccgaa cctcccgcac gatgtaccgc aattgggaca tcgagaacca catggagatc
     3061 gcccggctga tacagcagaa gctccttcct cagagccttc ccgatacccc ggggtaccgt
     3121 gcacacgccg cttacgttcc tatggacaag gtcggggggg acttctacga cataacgagg
     3181 tatggcgata ccatacgggt gttcatcgcc gacgtctcgg ggcacggcct cgccagctcc
     3241 tacctcgcga tgatcgcgcg gatgtcgctc gagagtgtct ccggcaaccg gtcgacgggg
     3301 gcggtcatca agcacatcaa cgaggccatc tgccgttcga cggtgaagaa caactacgtc
     3361 acctccttct atgccgagat cgatctctcg acgaacgtta tccgctacac caatgccggc
     3421 cagttcccgc cgcttgtcta ccgcgcgggc accggtgagt ttttcgagtt gatgacgcgg
     3481 ggacggccgc tcggctggtt cctgtcgctg ctggtcgagg agaagagttt ccagctcagt
     3541 tccggcgacc ggctgatcct cttcaccgac ggtatcatcg aatgcgcgaa ccggaagggg
     3601 gagatgttcg gcgaggaacg attcagggag ttcattcgaa gtcatgggca gcttcatacc
     3661 ggggagttcg ccgatcgtct gctgtcattg ctgaaggagc atcgtggaac cggcagcttt
     3721 gacgatgacg tgacgttgat agtgctggac gtcctgtgag gcgtgtcttc gctcgcggca
     3781 tcgaatcggg cagcacgtcg attgaatggc cttacccctt ccacatcacg aacaggtaat
     3841 tgaatccccg cagaaaaaaa tttccctctt ctgcggcctt gtggtagttc cccttcttga
     3901 agtccttgtt gtggcgggtc acgctgatga tgtccatcgg cgtgaattgt tcgctgagca
     3961 tgccgaagag gcggaagccg atgggcacga agccgtgctt ctgattgtag taatcgctca
     4021 cgtacaacgc catcgcgcgc ttgcgccgga gaatgcgatg aatctcgccg attgccgctc
     4081 gcatcgcctc gaaatagcgc tcatcggtcg ccgggagctt cccgatgcac ttcttctcgt
     4141 tcgagtagtc gatgttgtca ccgtaggggg gatccacgaa cacgaaatcg gccttctcgt
     4201 tctcgagcgg gagctttcgc gcatcggcgc ggaagatgtc cttccggctc gggttgatgt
     4261 cgtagcccag aaagcgccgc tccaggtcct tgcacacgtc gatggtggtg ccgctcccgc
     4321 acatggggtc caccacgaga tcgcccggct tggtgtagcg ctgcaggagg ttccagatga
     4381 tatacgacgg cgtagcgccg acgtaattct ggtcgccctg cggggcgttc ccgtaattct
     4441 gggacgggta gtcccagagc gtggtcgtct gtatgcggag cggtggcttg ggcatgattc
     4501 ccggtagcgc ccggcagggt atctgtcaag gaataatacg ggcgcgtcat tttcatcttg
     4561 accgcatgcc gtcgatgatg tcactgtgtc cgccgacaat cgcgtaagga tgctttgtgt
     4621 ggataaccta tttatctata tcagcaagca gctcaagttc gagaagtttc cccgctgctt
     4681 tttactcacc tgggtgttta tcgtaacggg cctgatggtt tttgtcctgt tccaattcct
     4741 tgccgggctc atgccgcggg ccggcttctt cgccctcttt cttcagttta tgggaatctt
     4801 cctgctgttc acctcgctcc tgatggcgta tgcgggcgtc tatgccatca ctacggcgcc
     4861 gcaggacgtg gccggcatca cccgggccgc aatcgtggcg acggtccgtc gctcctacat
     4921 gattttaggg gtgtcgttcc ttacgctgtt cgtgtatgtg gccttcctgc tgctgcaggt
     4981 gggcgtgtcg tccatcgcga tcattccgta cgtggggccc gtcgttatcg cgctgttcac
     5041 ggcgccgctg ctcctggtga accttgcggg cctcatcgcc gtcatcatcg tctttgcggt
     5101 cttcccgccg ctggccagcg aggtgcgcac catgcgggac ctttttctcg agttcaagat
     5161 atccgtccag ggccgctggc tcaaggtcgc cctctacacc ctggtgtcgt tcgcgctcct
     5221 cgtgctgtgc gtgctctgca tctattacct tctcatgtat gtggccggca tctcgcgggc
     5281 ggtgcagggg agcatcatca cggcgtatcc gtcgctcatg gacacgctcg cgatgaagag
     5341 cgcggtctcg gaggcgatcc gggagctggc cccccggggc gcgtcgatcg cgagcatgcg
     5401 cgaggcgcgc attctgttca ccgatcttca cggcatcctg tcgtttctag tgggcctgtc
     5461 gtacctggtc ctgttgtcgg cggtgatggc gtttccgctc gcggcctatt tcaatctctc
     5521 gtcgatcttt ttcggccggg tgaagcggta gggtgcggca gcgcggaaca gggatgatgc
     5581 cgcgaacggt cgtcggcatc gtgctgatat gcgccgcggt cgtcgcgccg catgcgctcc
     5641 atggcgcgga atgcgccgag ggcgattgcg caaacggcgt cgggcgcttc cggttcgatg
     5701 acggctccga atatgccggc tcctggcagg gcgggcgttt ccacggcgag gggacgatgt
     5761 gctatgcgga cggcgccgaa tatcgcggcg catggcggtc ggggaagcgg cacggtcggg
     5821 ggacgcatat cgcgcccgac ggctccgagt attgcgggac cttcgccgat gaccggccag
     5881 cgggcgaggg cgtatacctg gggatcgacg gaagacgccg tcgcgtgacc atccgggaca
     5941 ataagatggt gccgacgctc cgcctcgaaa acgaaaagcc tgaggacggc tgccagtacg
     6001 gagaggtgga ctacaagggg cgctatcacg gatggtatcg cgggcgaaag ttcgacgcct
     6061 tcaagccaca cgggttcggg gccatgacct ggcccaacgg cgcca
//
LOCUS       DAORHF010000221         6094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13035_length_6094_cov_4.216095, whole genome shotgun sequence.
ACCESSION   DAORHF010000221 DAORHF010000000
VERSION     DAORHF010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13035_length_6094_cov_4.216095"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKJ16_14880"
     CDS             complement(<1..292)
                     /locus_tag="PKJ16_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48326.1"
                     /translation="MVIRLIAVSAILCGSATAHAAAAKCVRGTCANGQGTLAYSSGDT
                     YTGFFVNGERSGFGTYAFLRGDTYTGQWRKDEKNGRGIYRFVNGESYDGEFRD"
     gene            complement(318..512)
                     /locus_tag="PKJ16_14885"
     CDS             complement(318..512)
                     /locus_tag="PKJ16_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48327.1"
                     /translation="MIEDAKKELTCPFCEEGTLVRRRCPACRKGHLVCDECESVYRDA
                     DSLDEELPSSECPHCGNPID"
     gene            complement(608..1117)
                     /locus_tag="PKJ16_14890"
     CDS             complement(608..1117)
                     /locus_tag="PKJ16_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48328.1"
                     /translation="MPQRSIRILLLMMLAATPLFARGDARFRLDAADIRALEKSGHLV
                     KIGADAWKTRGGALLTGRDPGGLTRLEHIMRHTVDAPGRPKHGVFSIGRADVIELLDR
                     VIARIRTGSAGAGKTRGDRVAYTVDAGSVVGYLGGREGKKRHHPKLTRVRLVVKKGAM
                     EVVTFFPVK"
     gene            1368..1976
                     /locus_tag="PKJ16_14895"
     CDS             1368..1976
                     /locus_tag="PKJ16_14895"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HNV48329.1"
                     /translation="MALQTPKGQQTREHVLESAMSLIRRKGYGNVSINDIIDATGVKK
                     GNLYFHFPGKDDLILAIIDRARRDYGAYLASRIRGATALERIDSLLAAVFEYHRRTRF
                     VGGCIFGNMALEMADADRRFADAIRAVFAEWTDSLERLLDEARRGGELATTLSTRTLA
                     KHIVAALEGAVMIAKVSKSARDFNDCLLPVRVLLGIESEVRQ"
     gene            1973..3130
                     /locus_tag="PKJ16_14900"
     CDS             1973..3130
                     /locus_tag="PKJ16_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006964258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HNV48330.1"
                     /translation="MTTKGEIINTARALGFEDIGFTTAEPFVTQRDLLANKRDEYDWA
                     TQVGLDLLAGTDPLSVLPTAKSIIVLLKAYWTESLPPSLEGHFGRCYLDDDRVTQDGM
                     ATKLKAFRGFLREHGIASKVPFNLPHRLAAARAGLGTFGKNCLLYANRVARRSSWVLP
                     IAFVVDREFVPDEPTARNACPDWCRNACVAACPTRALKGNGTIDPRRCISYLSYYGEG
                     ITPIELREAMGMYVYGCDRCQNVCPRNSAWTAQELPPNRRVQAKANAFDLAALLHMDR
                     AYFEANIWPHMFYMSADDIWRWKMNAARAMGNSLDERYVSELARAFRENDDERVRGMI
                     AWALGRIGGDAAIAVLDDIGRIDGGPSERICAEIETARVAASAPSCGHLST"
     gene            3147..3530
                     /locus_tag="PKJ16_14905"
     CDS             3147..3530
                     /locus_tag="PKJ16_14905"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HNV48331.1"
                     /translation="MDRNLPTAQQLDDLRNLPADTPFVMVNLLRYRDHADGTSGAAAY
                     QRYANVMLPMLAEYGARVLWAGKTKSVFIGGEDDRWDSVLLVEYPSAEAFLEMVSSPA
                     YASANEDRLLAVERTVLMVTQGVGL"
     gene            complement(3542..4114)
                     /locus_tag="PKJ16_14910"
     CDS             complement(3542..4114)
                     /locus_tag="PKJ16_14910"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV48332.1"
                     /translation="MCIEGATYHVFTRCIERRDLMRKDFYKEIMLDTLQLAQDKYSFD
                     LVFYEVLDNHIHLVITTRKGEATISRIMQYVKARFAEKYNRKNGRTGPFWNERFGDVI
                     VDLQGNPVFYFLWLLWYLAYNAVRKRAVHDPRAYKYGSINAYLDGSGDDRIKLTLHRF
                     FLELGDDIADRVKGFLGFEELYRKRLWMAF"
     gene            complement(4372..5640)
                     /locus_tag="PKJ16_14915"
     CDS             complement(4372..5640)
                     /locus_tag="PKJ16_14915"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNV48333.1"
                     /translation="MPPYDIVALSGKGIGYLVYLLIGMAFGAVLEMSGFGDSRKLSGQ
                     FYLRDMTVLKVMFTGIITAMLLIFGASALGLIDFEKVWVNHTFLWPQIIGGLVMGVGF
                     IIGGFCPGTSLVAASTLKIDGAMFVGGVFFGVFLFGETFGIMEPFYNAGDFGRFTLYE
                     LFGVSAGLVVFLVVVIALVMFTGAEVSERVFGRNVAWRRIDLNPTKLLNPVFIGVFMF
                     AALAVLIIPQPTLEDRWRFLSAEERAAFDRRDVFIHAGELNAVMNDTMLYTTILDIRS
                     EQDFNLFHLRNAKRISRADLYPDAGLVKKLQGAPGNMVIVLMSNEEAAAAEAYRLLRV
                     QGVPNLYVLAGGVNAWLDAFGVDRAIARKIDPKAVLPADSLHYRFTRAVGAGIPAADP
                     AFIMGNTCPVLTFDKKVKIVKKAAKTGGCG"
     gene            complement(5651..>6094)
                     /locus_tag="PKJ16_14920"
     CDS             complement(5651..>6094)
                     /locus_tag="PKJ16_14920"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HNV48334.1"
                     /translation="YFFTQSGLGASGAIGKVQIALVKLVAPGHVNRNSYFAKEGGGDK
                     NALDSGAVFMLLGTFLGGLASGLINRRVRPETRRGPQITDRQRWIFAFVGGAVMGYGA
                     RLARGCTSGQALSGGAVLAVGSWAFMLCVFIGGYALAYFVRRLWN"
BASE COUNT         1244 a         1943 c         1810 g         1097 t
ORIGIN      
        1 cgtcgcgaaa ttcgccatcg tacgactccc cgttgacgaa gcggtagatg ccgcgcccgt
       61 ttttttcatc tttgcgccac tgcccggtgt aggtgtcgcc gcgaagaaag gcataggtcc
      121 cgaatccgct ccgctcgccg tttacgaaga atcccgtgta ggtgtccccg ctggaatagg
      181 cgagggtccc ctgcccgttc gcgcaggttc cccgcacgca cttcgcggcg gccgcatgcg
      241 ccgtggcgct tccgcacaga atcgcggaca ccgcaatcag ccggatcacc attgggccgc
      301 acaccggttg cacaccgtca atcgattgga ttcccgcaat gcgggcattc cgaggagggc
      361 agctcctcgt cgagcgaatc cgcgtcccga tagaccgatt cgcattcgtc gcacaccagg
      421 tgcccctttc ggcaggccgg acagcgccgg cgaacaagcg tgccctcctc gcagaagggg
      481 caggtaagct cttttttcgc atcttcgatc atttcgcacc ctgcctgatt cgagcggcgc
      541 cgccgtgcgg gatcatgcgc cgtatggtga taggatgtac cgcaatggct cggcggccgc
      601 ccatcggtca cttcaccgga aagaaggtca cgacctccat cgcgcctttt tttacgacca
      661 ggcggactcg cgtgagtttt ggatgatgcc gcttcttccc ctcgcggccg cccagatagc
      721 cgaccacgga cccggcgtcg accgtgtagg ccacgcgatc gccgcgcgtc ttcccggcgc
      781 cggcgctccc ggtccggatc cgcgcaatga cgcgatccaa gagctcaatg acatcggcgc
      841 ggccgatgga aaagacgccg tgcttcggac ggcccggcgc gtcgaccgta tggcgcatga
      901 tgtgctcgag ccgggtgagc ccgccgggat cgcgccccgt gaggagcgca ccgccgcgcg
      961 ttttccacgc atcggcgccg attttcacga ggtggccgga tttttccagc gcgcggatat
     1021 cggccgcgtc cagccggaac cgggcgtcgc cgcgcgcgaa cagcggcgtt gccgcgagca
     1081 tcatcagaag gagaatgcgt atcgatcgct ggggcatggt cgcgcctcgc cggaaaaaaa
     1141 tcggaccgcg gtaacggcat acggcccggt accaccgcta tcaccggcaa cgcctttttc
     1201 aagacgattt tacccgctgc cacataaaaa gttcacgcat gttgatccgg catgccggtt
     1261 cggcaggaga ggcccgccgg cgcgggaggg agcaccccgc cgcaatcgaa tttatctctt
     1321 gacaatatcg cccggtggaa tattttgtac caaacggtcg gtatactatg gcactgcaga
     1381 cacccaaggg acaacagacg cgcgaacacg tgctcgaatc ggcgatgtcg ctcatccgcc
     1441 gcaaggggta cggcaacgtg agcatcaacg acatcatcga cgccaccggg gtgaaaaaag
     1501 ggaacctgta tttccacttc cccggaaagg acgatctcat cctggccatc atcgaccggg
     1561 cgcggcgcga ctacggcgcg tatctcgcct cgcgcatccg cggcgcgaca gcgctggagc
     1621 ggatcgacag cctcctcgcg gccgtcttcg agtatcatcg ccggacgcgc ttcgtgggcg
     1681 ggtgcatttt cgggaacatg gcgctggaga tggcggacgc ggaccgccgc ttcgccgacg
     1741 cgatccgggc ggtgttcgcc gagtggaccg attcgctgga acgcctcctc gacgaggcgc
     1801 ggcgcggcgg cgagctcgcc acgacgcttt ccacccggac gctcgcgaag cacatcgtgg
     1861 ccgcgctcga gggggccgtc atgatcgcca aggtgagcaa gagcgcgcgt gatttcaacg
     1921 actgcctgct gccggtccgc gtgctgctgg gaattgaatc ggaggtgcga caatgacaac
     1981 gaagggcgag atcatcaaca cggcgcgagc gctgggcttc gaggacatcg gatttaccac
     2041 cgccgaaccg ttcgtgaccc agcgcgatct gctcgcgaac aagcgtgacg aatacgattg
     2101 ggccacgcag gtgggcctcg accttcttgc cggcaccgat ccgctctcgg tgttgccaac
     2161 ggcgaaatcg atcatcgtac tcctcaaggc ctactggacc gaatccctcc ccccgtcgct
     2221 cgagggccat ttcggacgct gctacctcga cgacgaccgc gtcacgcagg acgggatggc
     2281 cacgaagctc aaggctttcc gcggattcct gcgcgaacac ggcatcgcct cgaaggtgcc
     2341 attcaatctc ccccaccgcc tggcggccgc ccgcgcgggg ctgggcacct tcggtaagaa
     2401 ctgcctgctc tacgccaatc gcgttgcgcg acgcagctcg tgggtgcttc cgatcgcctt
     2461 tgtcgtggac cgggagttcg ttccggacga accaactgcg cggaatgcct gccccgactg
     2521 gtgccggaac gcctgcgtgg ccgcctgccc caccagggcg ctcaagggca acggcaccat
     2581 cgacccgcgg cggtgcatct cctatctgag ctactacggg gagggcatca cgccgatcga
     2641 gcttcgggag gcgatgggca tgtacgtata cggctgcgac cgctgccaga acgtgtgccc
     2701 gcgaaacagc gcctggacgg cacaggagct tccgccgaac cgacgcgtgc aggccaaggc
     2761 gaatgccttc gaccttgccg ccctgctcca catggaccgg gcctatttcg aggcgaacat
     2821 ctggccgcac atgttctaca tgtcggccga cgacatctgg cgctggaaga tgaatgccgc
     2881 gcgcgcaatg ggcaacagcc tcgacgagcg ctacgtgagc gaacttgcgc gcgcctttcg
     2941 cgaaaacgac gacgagcgcg tgcgggggat gatcgcctgg gcgctcgggc gcatcggggg
     3001 cgatgccgcg atcgcggtgc tggacgatat cggccgcatc gatggcgggc cctccgaacg
     3061 catatgcgcc gagatcgaga cagcgcgtgt ggcggcatcg gcgccgtcat gcggacacct
     3121 atcgacataa ctcaggagag accgagatgg atcgaaacct acccaccgcg caacagctgg
     3181 acgatctgcg caatttgccc gccgacacgc cgttcgtcat ggtaaacctg ctgcgatatc
     3241 gggaccacgc cgacggcacg tccggcgcgg cggcctacca acgttatgcg aacgtgatgc
     3301 tcccgatgct tgccgagtac ggagcacggg tgctgtgggc ggggaagacg aaatccgtgt
     3361 tcattggcgg agaggatgac cgctgggact cggtgctgct ggttgaatat ccgtccgcgg
     3421 aggcatttct ggaaatggtt tcctcaccgg cgtacgcgtc tgcgaatgag gaccggttgt
     3481 tggctgtgga gcggacggtg ctcatggtca cgcaaggggt ggggctctga ccctcttttc
     3541 attagaaggc catccagagc cttttccggt acaactcctc gaaaccgaga aaccctttca
     3601 cgcggtcagc gatatcatcc cccagttcca ggaagaagcg atggagcgta agcttgattc
     3661 tatcgtcgcc gctgccatca agatatgcgt ttatactccc atacttgtac gcgcgcggat
     3721 catgcaccgc ccttttccgg acagcattat acgccaggta ccacaacagc cacaaaaagt
     3781 agaataccgg gttgccttgg agatcaacaa tcacatcccc gaagcgttca ttccaaaacg
     3841 ggccggttct cccgtttttc cgattatatt tttcagcaaa acgagctttg acatactgca
     3901 ttatcctcga aatcgtggcc tcaccttttc gtgttgttat aaccagatgg atatgattat
     3961 cgagaacctc ataaaacacg agatcaaatg agtacttatc ttgcgccagt tgaagcgtat
     4021 cgagcatgat ctccttgtaa aaatcttttc gcataagatc ccttctctcg atacaccgtg
     4081 taaacacatg atacgttgct ccttcgatgc acatcctcgt agatcggggc attgctccct
     4141 ccggtaaaca ggttttttcc actgcttact gtaaatacga ggctggattg ctgatctata
     4201 aaaaaatatg atggaatgtg tccgccgaag ccccagaaag cacagaaggg gtcagagcat
     4261 ggtatggtat atggtatggt atatggcatg gtacaaatct tttaattgag aatcgacgaa
     4321 taatccaatg aggctctgac cccttcctcc aagggaaccc caggccaccc ctcacccaca
     4381 cccgccggtc tttgcggcct tctttacgat cttcaccttc ttgtcgaagg tgagcaccgg
     4441 acaggtattg cccatgataa acgccggatc ggccgcaggg atccccgcgc cgaccgcgcg
     4501 cgtaaaccgg tagtgcagtg aatcggcggg aaggacggct ttgggatcaa tcttgcgggc
     4561 gatggcacgg tccaccccga aggcgtccag ccaggcattg accccgccgg caagcacgta
     4621 caggttgggc accccctgaa cccgcagaag gcgataggcc tccgccgcgg ccgcctcttc
     4681 gttcgacatg agcacgatga ccatgttccc gggcgcgccc tgcaattttt tcacgagccc
     4741 cgcgtcggga tacaggtcgg cgcggctgat tcgcttcgcg ttgcgaagat gaaacagatt
     4801 gaaatcctgt tcgctgcgaa tatcgagaat ggtcgtgtag agcatcgtgt cgttcatgac
     4861 cgcgttgagc tcgcccgcat ggatgaagac atcgcggcga tcgaaggccg cccgctcctc
     4921 ggcgctcaag aatctccagc gatcctccag cgtgggctgg gggatgatga gcacggccag
     4981 cgccgcaaac atgaacacgc cgatgaacac gggattgagc agcttcgtcg gattgaggtc
     5041 tattctgcgc cacgcaacgt tgcgcccgaa aacgcgttca ctcacctccg cgccggtgaa
     5101 catcaccagc gcaatcacga ctaccaggaa aaccacgagt cccgctgaaa ctccgaagag
     5161 ctcatagagg gtgaagcgcc cgaagtcccc cgcattgtaa aacggctcca tgattccgaa
     5221 ggtctcgccg aagaggaaca cgccgaagaa aacgccgccc acgaacatgg cgccgtcgat
     5281 cttgagcgtg gaggcggcga ccaacgaggt ccccgggcaa aagcccccga tgatgaaacc
     5341 gacgcccatc accagccccc cgataatctg cggccacagg aaggtgtgat tgacccacac
     5401 cttctcgaaa tcgatgaggc cgagggccga ggcgccgaag atgagcagca tggcggtgat
     5461 gatgccggtg aacatgacct tgaggacggt catgtcgcgc agatagaatt ggcccgaaag
     5521 cttgcgcgaa tcgccgaatc ccgacatctc gagcacggcg ccgaaggcca tcccgatcag
     5581 gaggtacacc agatacccga tccccttccc ggaaagcgcg acgatatcat acggcggcat
     5641 tgccatcacc tcaattccac agccgtcgga cgaaatacgc gagcgcgtac ccgccgatga
     5701 acacgcacag catgaacgcc cagctcccga cggcgagcac cgcgccgccg gagagcgcct
     5761 gccccgaggt gcatccccgc gccaggcgcg cgccgtaccc catcacggcc ccgccgacga
     5821 aggcgaagat ccatcgctgg cggtcggtga tctgcggccc gcgccgggtc tcgggccgca
     5881 cgcgccgatt gatcagtccc gaggccaggc cacccaggaa ggtgcccagc agcatgaaga
     5941 cggcccccga gtcgagggcg ttcttgtcgc cgccgccctc cttcgcgaag taggaatttc
     6001 ggttaacgtg ccccggggcc acgagcttca ccagggcgat ctggaccttg ccgattgcgc
     6061 cggaggcgcc gagcccgctc tgggtgaaaa aata
//
LOCUS       DAORHF010000222         6087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13055_length_6087_cov_4.208389, whole genome shotgun sequence.
ACCESSION   DAORHF010000222 DAORHF010000000
VERSION     DAORHF010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13055_length_6087_cov_4.208389"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(223..1119)
                     /locus_tag="PKJ16_14925"
     CDS             complement(223..1119)
                     /locus_tag="PKJ16_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV48335.1"
                     /translation="MMILQPLIDGLAIGSLYALIALSMVIIYKTSEVPNFAQGEMAML
                     STFFAYYLMENAGFAFPYAFLGALVFAAVVGVVFEFVFLRREKEVGILGFILITLGFE
                     RILYGIAGWKWGSEQKLFPSSFAPLPVSGSTMIHLGGVSISQIKLIIVIVAVSLIGLI
                     FLFFKYTRVGIAMKATQQNATAARINGIRTKRIMSLTWAMSAVVGAIAGMLLVPGTNT
                     SLDANIMLAPLLKGFAAAVLGGMNTLVGSIGGGIILGVIEKFFGFYISSEFETVVAFL
                     IIVIILWFRPGGLFGRHYVRKV"
     gene            complement(1136..2224)
                     /locus_tag="PKJ16_14930"
     CDS             complement(1136..2224)
                     /locus_tag="PKJ16_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV48336.1"
                     /translation="MDIKRDYYEDIRLLGSGAKRFWFGVMMLALAVTPFLLPGHVLSD
                     VNFIAINVIVAIGLNILVGFTGQVSLGHAGFFAIGAYATVLLMSAAQFVPFPLALLAA
                     GLIAALFGFVLGLPSLKLEGPYLAIATLGFGMAITQIIGRFSVFGGRDGIQVPEVHYL
                     DRLFVEYAGMTASAAKAADLYFIIIVFAVAMTIMARNIIKTRVGRAFIAIRDDDIAAQ
                     SMGVHVTLYKTLSFAVSAFFTGVAGGLYAIWLGYINPESFGFIMSVLFLAMVVVGGVG
                     SIMGSVLGAIVISFLDLNLKQNTIVQIPVLGNVLVMFTDAVMDTNGIAYVSYIIFGLI
                     MMLIVVFEPLGLFGFWIRIKKYWKTWPF"
     gene            complement(2270..3484)
                     /locus_tag="PKJ16_14935"
     CDS             complement(2270..3484)
                     /locus_tag="PKJ16_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV48337.1"
                     /translation="MNRFYSFIIAMAFASFLILPGCAKKAVDERRGVTDTTVKIGQWG
                     PQSGPAALWGAVARGSDLYFKIINEEGGIAGRKIQHFIRDDGYQPNRTKSEVKQLVEG
                     EGVFAFVGGLGTATGMAVKQYLNDNKVPWVNPSSPSSHWTFPQSKYLFNFFPLSSTEA
                     DLLLKYAMENLKKNSFSIFYQNDDYGKLGYLAAKHILEKKGLKLAEGVSVENADTDLS
                     SHALKLKNANADVVIMWVNPKHASIILGQCANIGYKPQWMASSTLSDMAIMYKISRGL
                     WKDMIFANFTESPDSDSPLMKKYRAYHQKFAPNERWGVFYYAGIMFAEPMVEALKKCG
                     KDLTVENFVKAMESIKDFQGIGPKITYGPNQRQGANSIFIAKCISDTKAQKLTGWLTS
                     DFDVNEILKQVK"
     gene            complement(3732..5528)
                     /locus_tag="PKJ16_14940"
     CDS             complement(3732..5528)
                     /locus_tag="PKJ16_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006421010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48338.1"
                     /translation="MNTVPEIFTATVSKYGDRVALRFKQYGTWRDVSWNEYLERATSV
                     ACGLYALGFRKGDRVAIIGDNCPEWVYVDLGTMCAGGVSVGVYATNSWDQCEFVVNHA
                     EAKFYFMENEEQLDKWLQFRENAPTLATVFVWDLKGLRDFRDPLLMTFDELVELGKKT
                     AEEQPDLMNALMAAVRPDDDALFIYTSGTTGRPKGAVLTHANIAWIANAIQMANPLYD
                     TDEVLSYLPLCHVFERIFTVFAHVRYGYIVNFIENLDTITDNMREISPTVGYAVPRIW
                     EKYLSMVIIKMSDATWLKKHIFKAALDVANRRAQLMQDGNPVPLALALLYRIAYIAVF
                     RKLKERLGFDRMRLAYSGAAPISTGVITFFNAIGVPLLEGYGQTEGTGVTTVNRLGSS
                     RIGTVGRPIDGVEVRIGEDDEIIVKSPGVFKGYYRDEESTRQSIIDGWLHTGDVGAID
                     HEGNVIITGRIKDIIITDGGKNITPQFIEGKLVFSPYVNDAIVIGDKRKYLTAIIVLD
                     EDNVVKYAQDHKIQFSTYRDLAQNLEIEKLIQKEVDAVNEAVSRVEQIKRFTILPHKL
                     YQEEGDVTPTMKVKRRYIQEKYADLIDGMYRG"
     gene            complement(5695..>6087)
                     /locus_tag="PKJ16_14945"
     CDS             complement(5695..>6087)
                     /locus_tag="PKJ16_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV48339.1"
                     /translation="LKERTGQLAGTLSGGEQQMLAIGRALMNAPKLLLLDEPSLGLSP
                     LLVKTIFGIIKTIRDDGVTVILVEQNARMALQIADFGYVMENGRFVMKGTARELMEDK
                     DVQEFYLGVKTDVSAKGYQRWKRKKRWR"
BASE COUNT         1282 a         1878 c         1710 g         1217 t
ORIGIN      
        1 atcaaagata gctcgatcgg tcgcgctcgt gtattcgctc caatcgtcgc tcatcccggc
       61 gatcccgaag acgtcggtaa tcgaacgaac gtagcactcg gcatcgacgt ccgtggagtt
      121 cagcagcgtc ccgtcgatat cgaaaatcac gagcttcatg gttttacagc gccgaaggcg
      181 tcgcgagaaa ggtcgttcgt tgtacggacg tcggaaattg gcttacacct tccgcacata
      241 gtggcgcccg aagaggcccc ccgggcgaaa ccagagaatg ataacgatga tgagaaaggc
      301 gaccaccgtc tcgaattccg aagagatata gaacccgaag aacttctcga tcacccccag
      361 tatgattccg ccgccaatcg atccgacaag cgtgttcatc ccgcccagca cggcggcggc
      421 gaagcccttg aggagcggcg cgagcatgat gttcgcgtcg agcgaggtat tggtgccggg
      481 caccagcagc atgcccgcga tcgcgcccac caccgcgctc atggcccagg tgagcgacat
      541 gatgcgcttc gtacggatgc cgttgatgcg cgccgcggtc gcattctgct gggtcgcctt
      601 catggcgatg cccacccggg tgtacttgaa aaacaggaat atcagcccga tgagcgaaac
      661 cgcgacaatc acgatgatga gcttgatctg gctgatgctc acgcccccca ggtgaatcat
      721 cgtggagccc gaaaccggca gcggcgcgaa gctcgatgga aacagcttct gctccgatcc
      781 ccatttccag ccggcgatgc cgtacaggat gcgctcaaaa ccgagggtga taaggatgaa
      841 gccgaggatg cccacctcct tctcgcgtcg caggaaaacg aactcgaaca cgacgcccac
      901 cacggccgca aaaaccaggg ctcccaggaa cgcgtacgga aaggcgaacc ccgcattttc
      961 catgagataa tacgcgaaaa acgtcgagag catggccatc tcgccctgcg cgaaattggg
     1021 cacctcggag gtcttgtaga tgatgaccat cgaaagcgcg atgagggcgt agaggctccc
     1081 gatcgccagg ccgtcaataa ggggctgcag aatcatcata cggttttccc cgatcctaga
     1141 aaggccacgt cttccagtat tttttgattc ggatccagaa cccgaacaat cccaacggct
     1201 cgaagacgac gatgagcatc atgatgaggc cgaagatgat gtagctcacg taggcgatgc
     1261 cgtttgtgtc catgaccgcg tcggtgaaca tgaccagcac attcccgagc accggaatct
     1321 ggacgatggt gttctgcttg agattgaggt ccaggaacga tatgacgatc gcgcccagca
     1381 ccgatcccat gatggagccc acaccgccca ccaccaccat cgccagaaaa agcaccgaca
     1441 tgatgaaccc gaacgactcc gggttgatat agccgagcca tatcgcatac aggccgcccg
     1501 ccacgccggt gaaaaacgcg ctcacggcga aggagagggt cttgtagagc gtgacgtgaa
     1561 cgcccatcga ctgggcggcg atatcgtcgt cgcggatcgc gatgaatgcg cgccccaccc
     1621 gcgttttgat gatattgcgc gccatgatcg tcatggcgac ggcgaagacg atgatgatga
     1681 aatacaggtc ggccgccttg gccgccgatg cggtcatccc ggcgtactcg acgaacaggc
     1741 ggtcgagata gtgcacctcg gggacctgga tgccatcgcg tccgccgaag accgagaagc
     1801 ggccgatgat ctgcgtgatg gccatgccga aacccagcgt ggcgattgcg aggtacggcc
     1861 cctccagctt gagcgacggc agtcccagga cgaacccgaa cagcgccgcg atgagcccgg
     1921 ccgcgagcag cgcgagcggg aacgggacga actgcgcggc gctcatcaac aacaccgtcg
     1981 cataggcccc gatcgcgaag aagcccgcat ggcccaggga cacctggccg gtgaacccca
     2041 ccaggatgtt caatccaatc gccacgatca cgttgatggc gatgaagttc acatcgctca
     2101 acacatgacc gggcaacagg aacggcgtca cggcaagcgc gagcatcatc acgccgaacc
     2161 aaaaccgctt tgcgccgctc cccaggagcc gtatgtcctc gtagtaatcg cgtttaatat
     2221 ccatggcgtg tgacgcgggg cccgatgagc gacgggcccc gttccccgtc tatttcacct
     2281 gtttcaggat ctcgttcacg tcgaaatcgg acgtgagcca gccggtgagt ttctgcgcct
     2341 tcgtgtcgct gatgcatttg gcgatgaaga tcgagttcgc gccctggcgt tggttgggcc
     2401 cgtaggtgat cttggggccg atgccctgga agtccttgat ggactccatc gccttcacga
     2461 aattctccac cgtgaggtcc ttcccgcatt tcttgagcgc ctcgaccatc ggctcggcga
     2521 acatgatgcc ggcgtagtag aagacccccc agcgctcgtt gggagcgaac ttctgatgat
     2581 acgcgcggta cttcttcatg agcggggaat ccgaatccgg cgactcggtg aagttcgcga
     2641 agatcatgtc cttccagagt ccgcgcgaga tcttgtacat gatggccatg tccgacagcg
     2701 tactggaggc catccactgc ggcttatagc cgatgttcgc gcactggccc aggatgatgg
     2761 acgcgtgctt ggggttcacc cacatgatca ccacgtcggc gttcgcgttc ttcagcttga
     2821 gcgcgtgcga gctcaaatcg gtgtcggcat tctcgaccga cacgccctcg gcgagcttca
     2881 gtcccttctt ctcgaggatg tgcttggcgg cgagataacc gagctttccg taatcgtcgt
     2941 tctgatagaa aatcgagaag gagttctttt tcaggttctc catcgcgtac ttgagcaaga
     3001 ggtccgcttc ggtgctcgaa agcgggaaaa agttaaaaag gtacttggac tgcggaaagg
     3061 tccagtgcga cgagggcgac gaagggttca cccacgggac cttgttgtca ttgagatact
     3121 gcttcacggc catgccggtc gcggtcccca ggccgccaac gaaggcgaac acgccctcgc
     3181 cctcgacgag ctgcttcacc tcggatttgg tccgattcgg ctgatagccg tcgtcgcgga
     3241 taaaatgctg aatctttcgc ccggcgatcc cgccctcttc attaatgatt ttgaaataca
     3301 ggtccgatcc gcgggcgacc gcgccccaga gcgccgccgg ccccgattgc gggccccatt
     3361 gcccgatctt cacggtcgta tccgtcacgc cacgccgttc gtcaacggcc ttcttcgcgc
     3421 agcccggcag gatcaggaac gatgcaaacg ccatcgcgat gatgaatgaa tagaatctgt
     3481 tcataactct cctccagtga tatcgtgtgt tcttgttgta taaagcaatg tcaatgcgcg
     3541 cacagcgaat cagcccctcc gtagtgccgc tccagcatca acgaactgtt gtaaaaaaac
     3601 ctcattccaa aattctccgc gggaagtttc acaatttttg gaacgttttt catccgcact
     3661 gatttcaacg ccccctcccg atctcccact tcatttgggg cggtgcgcca atggtgaccc
     3721 gagacagccc cttatccacg atacataccg tcaatcaggt ccgcatactt ttcctgtatg
     3781 tagcgacgct tcaccttcat ggtgggggtc acgtccccct cctcctgata cagcttgtgc
     3841 ggcaatatgg tgaatcgctt gatctgctcc acgcgcgata cggcctcgtt caccgcatcg
     3901 acctccttct ggatgagctt ctcgatctcg aggttctgcg cgagatcgcg gtaggtcgag
     3961 aactggatct tgtggtcctg ggcgtacttc acgacattgt cctcgtccag gacgatgatg
     4021 gcggtgagat acttccgctt gtcgccgatg acgatcgcgt cgttcacgta gggactgaac
     4081 accagcttgc cctcgatgaa ctgcggcgtg atgttcttcc cgccgtcggt gatgatgatg
     4141 tccttgatgc gtccggtgat gatgacattg ccctcgtggt cgatcgcccc cacgtcgccg
     4201 gtgtgcagcc agccgtcgat gatggactgg cgcgtcgatt cctcgtcccg gtagtacccc
     4261 ttgaagacgc ccggcgattt cacgatgatc tcgtcatcct ccccgatccg cacctcgacc
     4321 ccgtcgatgg gcctccccac ggtgccgatg cggctggaac cgagccggtt cacggtggtc
     4381 acgcccgtcc cctccgtttg accgtaccct tcaaggagcg gcacgccgat ggcgttgaaa
     4441 aacgtgatca cgcccgtcga aatgggcgcc gcgccggaat aggcaagacg catccggtcg
     4501 aagcccaaac gctccttcag cttccggaac accgcgatat acgcgatgcg atagaggagc
     4561 gcgagtgcga gcggaacggg gttcccatcc tgcatgagct gggcgcgacg gttcgcgaca
     4621 tcgagggccg ccttgaaaat atgctttttc aaccaggtcg cgtccgacat cttgataatg
     4681 accatggaaa gatacttctc ccagattcgc ggtacagcgt acccgaccgt gggagagatc
     4741 tcgcgcatgt tgtcggtgat ggtgtccagg ttctcgataa aatttacgat atacccgtat
     4801 cggacgtgtg cgaacacggt gaagatccgc tcgaagacat ggcagagcgg cagatacgac
     4861 aggacctcgt cggtatcata cagcgggttc gccatttgga tggcgttcgc gatccaggcg
     4921 atgttcgcat gcgtgagcac cgcgcccttg gggcgccccg tggtgccgga ggtgtagatg
     4981 aacagcgcgt cgtcatccgg ccgcacggcc gccatgagcg cgttcatgag atcgggctgc
     5041 tcctcggcgg ttttctttcc cagctccacg agctcgtcga aggtcatcaa caggggatcg
     5101 cgaaagtccc gcagcccctt gaggtcccac acgaacaccg tggcgagcgt tggcgcgttc
     5161 tctcggaatt gcagccactt gtcgagctgc tcctcgttct ccatgaaata gaacttcgcc
     5221 tcggcgtggt tgacaacgaa ctcgcactga tcccacgagt tcgtggcgta caccccgacg
     5281 ctcacgccgc ccgcgcacat cgtcccgagg tccacataga cccattccgg gcagttgtcc
     5341 ccgatgatgg cgacgcgatc acccttacga aaacccagcg catacaggcc gcaggccacg
     5401 gaggtcgcgc gctcaaggta ctcgttccac gacacatcgc gccaggttcc gtactgcttg
     5461 aaccgcagcg ccacccgatc gccgtatttc gatacggtcg cagtaaaaat ttccggtacg
     5521 gtgttcatat atacattcgt ttcatttttt gcatactaat aacgttgata acgcatctcg
     5581 agtgtcccga tactcgtacc aatcatcaag catttaacgc gacaaacccc atagccatcc
     5641 ctccctatcg aagggggagg tgtccgaagg acggaggggc cagagccccc cgcatcaacg
     5701 ccaccgcttt tttcgcttcc agcgctgata gcccttcgcc gaaacgtccg tcttgacgcc
     5761 caggtagaac tcctggacat ccttgtcctc catgagctcc cgcgccgtgc ccttcatcac
     5821 gaagcgcccg ttctccatca catagccgaa gtccgcaatc tggagcgcca tacgcgcatt
     5881 ctgctcgacc aggatcaccg tcacgccgtc gtcgcgaatc gttttgatga tgccgaagat
     5941 ggtcttgacc aacagcggcg aaaggcccaa cgacggctcg tcgagaagca gaagcttcgg
     6001 cgcgttcatg agcgcgcggc cgatcgcgag catctgctgc tcgccgccgg agagcgtccc
     6061 ggcaagctgg ccggtgcgct ccttgag
//
LOCUS       DAORHF010000223         6057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13142_length_6057_cov_4.804232, whole genome shotgun sequence.
ACCESSION   DAORHF010000223 DAORHF010000000
VERSION     DAORHF010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13142_length_6057_cov_4.804232"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1042)
                     /locus_tag="PKJ16_14950"
     CDS             complement(<1..1042)
                     /locus_tag="PKJ16_14950"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HNV48340.1"
                     /translation="MKTQRNLIAQTIIVCLIAAGPATALELKKTIHGELAAKSVVHNG
                     KGLFFAQNMMYRHTISVYNSDYQLVKTIPDTIRPSDYGYTNLKGTFRGAPVECAFSHD
                     GQFAWISQYCTTGAGRNNPGHDKCGPSSGYDNSFLYRINTTTFAIDAIVEVGSVPKYV
                     GTSPDNKYVIVTNWCTYDAHIIDTATNKIVKKIYLGPYPRGIVVDPTSSFAYIAVMGS
                     YDIAIIDLKTFEKNWIKGVGRSPRHLCISPDGRYLYASLNGEAQLAKVDLLSGKNVAK
                     VHTGSQPRSMDISTDGAFLYVVNYSSNSMSKVRTADMKVMKTVKTNGAPIDITYDGIK
                     KEVWVSCYSGTLM"
     gene            1398..2744
                     /locus_tag="PKJ16_14955"
     CDS             1398..2744
                     /locus_tag="PKJ16_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNV48341.1"
                     /translation="MDAILKKIEKLAVTHDRVSPEIIAEKNIKLGLRNQDGSGVIVGI
                     TSKGEVLGYRRTPLPGGKGFSVDPVPGKLYYCGYDVEELVTRIHAEKRFGFDEITYLL
                     LTGALPATPDLDRFTRVLAKRRTLSKIERRIFLDELANDNQMYALHSVISHLSLTDKN
                     PDSTDIKDVSVQCINLIAKTPTIVAYNYNAMRFRKGGNLTIVHPDPRLSTAENFLYML
                     KGERPSEFEAYLFDLCLMLHAEHGGGNNSTFTVRTVSSSGANTYMAICAGIASLSGHL
                     HGGANEAVMMMMADVKRNVKHWDDDDEIAAYLAKILAGKAHDRSGKIYGMGHAVYTTS
                     DPRAVILREKAEQYAAIHGVEDEFRLFSKVADIGARLLQKDKGKVVAPNVDFYSGFVY
                     KHMGIPIELFTPIFAMARIVGWSAHRIEQIIQAKLIRPAYLSSLGEKPNYLPLDER"
     gene            complement(2741..3685)
                     /locus_tag="PKJ16_14960"
     CDS             complement(2741..3685)
                     /locus_tag="PKJ16_14960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48342.1"
                     /translation="MYQVLIVDFSVYKITLEKIFTEAGYATQVCESAFEAMQKLRAYD
                     FDLIVSEVELPGDNAFDLYNYIAATYPYIPTIMTTDKMIDTIFDRIFSEGIGNVLCKP
                     VKKDDILTLADKLITKEKIFGLGNYLRGIRDERHIRITASSQIQKAIPVVLSQIQQWG
                     HSLENRMVLNLLLNEMTINAVYHSHGYTREKESRVPIELEDGKFVDIAYADTGASFGI
                     SISDYNGTLTKERILGAINSVIVQNRELLKASETGGDISNIIAETGRGIDLVRKLCGE
                     YYFIIKRGVRTEIIMLFDKDFVYDNDAYTSLKIIEDFA"
     gene            3767..4924
                     /gene="dprA"
                     /locus_tag="PKJ16_14965"
     CDS             3767..4924
                     /gene="dprA"
                     /locus_tag="PKJ16_14965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV48343.1"
                     /translation="METQNDSILMAVAISIVSSPGRRDLWREMRATEPGELYARIAAG
                     EPPAVQAHVARQYAGTPLEAAKRIREMAERRDVRIVSMWDDSYPSLLREIYRPPLVLY
                     VRGDPAFDRMIAIVGTRAADAGSASIARLISTELTAAGLCVVSGMAVGIDREAHMGAL
                     LQGRRTVGVLANGIDIRYPRRNADIYQAIIGTPGCGLVSEYPPGIIADRWTFVRRNRI
                     ISGLSHGVVVVKAGDKSGALITAGYAVEQNREVFACPGGSFNPDYAGCHRLIRNGAHL
                     VSDTRDILAELGPVAGDLRTAPAASCVCALSADHAAAKADEATGRCDDTASLSDVERV
                     VVGLVTSGSWEIDTLIRSSGFATDRVTEALIMLEMAGYIARDGTRIRLLQK"
     gene            4988..5614
                     /locus_tag="PKJ16_14970"
     CDS             4988..5614
                     /locus_tag="PKJ16_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48344.1"
                     /translation="MAIKRTFIVLWATLLVVSLSQLFPIPPYGEAVYSLYTSGYFVEL
                     DRQFRVVTDAFLGVKMMSRPAYAWVLLDDIEKAHGIRIRVYNARGVRVTAPGAPEGGV
                     DVDVQNALAAPKPSVASWVSRGSYYSILPLANEGQCGICHTGTYRNNMIGAVTFKRRC
                     DSRTFYAFERIAVFVGFSLVFALLLFATVRWDPQKSVRELFVRVDKNE"
     gene            5659..>6057
                     /locus_tag="PKJ16_14975"
     CDS             5659..>6057
                     /locus_tag="PKJ16_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013244953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HNV48345.1"
                     /translation="MSNSKEGKTLVIVESPAKAKTINKYLGSSYTILSSMGHIIDLPK
                     SRLAVDIDNDFVPEYITIRGRAKILNELKKNAAKSSRVLLATDPDREGEAISWHLANA
                     LTPKNANIQRIEFNEITEHAVKEAVQHPRSI"
BASE COUNT         1155 a         1755 c         1924 g         1223 t
ORIGIN      
        1 ccatgagcgt cccgctgtag caggagaccc acacctcttt cttgatgccg tcataggtga
       61 tgtcgatggg ggctccgttg gtctttacgg ttttcatcac cttcatgtcg gcggtgcgga
      121 ccttgctcat cgaattggac gagtagttga ccacgtacag gaaggccccg tcggtggaga
      181 tgtccatgga gcgcggctgg ctgccggtgt ggaccttcgc gacgttcttc ccgctcagga
      241 ggtccacctt ggccagttgg gcctcgccgt tgaggctcgc gtagaggtag cggccatcgg
      301 gcgagataca gaggtggcgg ggcgagcggc ccacgccctt gatccaattc ttctcgaatg
      361 ttttcaggtc gatgatggcg atatcgtatg agcccatcac cgcgatgtag gcgaacgagg
      421 aggtgggatc cacgacgatg ccgcgcggat agggccccag gtaaatcttc tttacgatct
      481 tgttcgtcgc ggtgtcgatg atgtgcgcgt cgtacgtgca ccagttggtc acgatgacgt
      541 acttgttgtc gggggaggtg cctacgtact tgggcaccga gccgacctcg acgatggcgt
      601 cgatcgcgaa ggtggtcgtg ttgatgcggt ataggaagct gttgtcgtat ccgctcgacg
      661 gcccgcactt gtcatggccg ggattgttgc ggccggcccc cgtggtgcag tattgggaaa
      721 tccacgcgaa ttggccgtcg tgggagaagg cgcattcgac cggcgctccg cggaaggtgc
      781 ccttcaggtt cgtgtagccg tagtccgacg ggcgtatggt gtcgggtatg gtttttacca
      841 gctggtagtc gctgttgtac accgatatgg tgtgacggta catcatgttc tgggcgaaga
      901 acaggccctt cccgttatgg acaacggatt ttgccgcgag ctcgccgtgt atggtcttct
      961 tgagctcgag agccgtcgcc ggccccgcgg cgatcaggca gacgatgatt gtttgtgcga
     1021 tgaggtttcg ttgtgttttc ataacgcaac cctatgagcg cgcgtcggag tgtcaagaaa
     1081 aagactatag catcgcttaa tttttgatcc gcacgtgcgc ccgcattgtt tcgtcgaggt
     1141 aaaatcgtct ggcatgagga aataatatct ggacaccgat gctcatcgcg cgccacggtt
     1201 catgcgtggc atgaaagctg cgacggaggt gtccaatgca tatcgagagg aatctacttg
     1261 acgctgcatc atccgttgta aagtatccac ttctgtgcga tggtcgcgaa cgaacgagcg
     1321 ccgggcaaat cgtcacgcga gcgcccgcag ttccgatcac ggtgacggag tcacggcccg
     1381 atcacgacga ggtgaacatg gacgccatat tgaagaaaat cgagaagctt gccgtaacgc
     1441 acgaccgcgt ctcaccggaa atcatcgccg agaaaaacat caagctgggg ttgcgcaacc
     1501 aggacgggtc cggcgtgatc gtggggatca cctcgaaggg cgaggtgctg ggctatcgtc
     1561 gcactccgct tcccggcggg aagggatttt cggtggatcc ggttcccggc aagctgtact
     1621 attgcgggta cgacgtcgag gagcttgtaa cgcgcattca cgcggagaag cgcttcggat
     1681 tcgacgagat cacctatctg ctgctcaccg gcgcgcttcc cgcaacgccc gacctcgatc
     1741 ggtttacgcg ggtgctcgcc aagcgccgga cgctctcgaa gatcgagcgc cggatcttcc
     1801 tcgacgagct cgcgaacgac aaccagatgt acgcgctgca cagcgtgatc tcgcacctgt
     1861 cgctcaccga caagaacccc gattcgaccg acatcaagga cgtgagcgtg cagtgcatca
     1921 acctgatcgc caaaacgccc acgatcgtcg cgtacaatta caacgcgatg cgcttccgca
     1981 agggcggcaa tctcaccatc gtccatccgg acccgcgcct cagcacggcg gaaaatttcc
     2041 tctacatgct caagggcgag cgtccctccg agttcgaggc gtatctcttc gacctgtgcc
     2101 tcatgctcca cgcggagcac gggggcggga acaactcgac cttcaccgtc cgcacggtgt
     2161 cttcaagcgg cgcgaacacc tacatggcca tctgcgcggg gatcgcgtcc ctgagcgggc
     2221 acctgcatgg cggcgccaac gaggcggtca tgatgatgat ggccgacgtg aagcggaacg
     2281 tgaagcattg ggacgacgac gacgagattg cggcctacct cgcgaagatc ctcgccggaa
     2341 aggcgcacga ccggtcggga aagatctacg ggatgggaca tgccgtgtac accacgtccg
     2401 atccgcgcgc cgtcatcctg cgcgaaaagg cggaacaata cgcggcgatt cacggcgtcg
     2461 aggacgagtt ccgcctgttc tcgaaggtcg ccgacatcgg cgcgcggctg ctgcaaaagg
     2521 acaagggcaa ggtggtggcg cccaacgtcg atttctactc cgggttcgtc tacaagcaca
     2581 tggggatccc catcgagctg ttcacgccga tcttcgccat ggcgcgcatc gtgggctggt
     2641 ccgcgcaccg tatcgagcag atcatccagg cgaagctcat ccgccccgcg tacctgtcgt
     2701 cgctgggcga gaagccgaac tacctgccgc tcgacgagcg ttaggcgaag tcctcgatga
     2761 tcttcaggga cgtgtacgcg tcgttgtcgt agacgaaatc cttgtcgaag agcatgatga
     2821 tctccgtccg gacgccgcgt ttgatgatga agtagtactc cccgcagagc ttgcgcacca
     2881 ggtcgatccc ccgcccggtc tcggcgatga tgttcgagat gtccccgccc gtctccgacg
     2941 ccttcaggag ctcccggttt tgcacgatga cgctgttgat ggcgcccagg atgcgctcct
     3001 tggtgagcgt cccgttgtag tcggagatgg agatgccgaa ggacgcgccg gtgtccgcgt
     3061 atgcgatatc gacgaacttg ccgtcctcaa gttcgatggg aacgcggctc tccttttcgc
     3121 gcgtgtagcc gtgcgagtgg tacaccgcat tgatggtcat ctcgttgagc agcaggttga
     3181 ggaccatgcg attttcgagc gagtgccccc actgctggat ctgcgagagc acgacgggga
     3241 tcgccttctg gatctgcgat gaggccgtga tgcggatgtg ccgctcgtcg cggatgcccc
     3301 gcaggtagtt tcccagcccg aagatctttt ccttcgtgat gagcttgtcg gccagcgtga
     3361 ggatgtcgtc ctttttcacc ggcttgcaca gcacgttccc gatcccctcg ctgaatatgc
     3421 ggtcgaagat ggtgtcgatc atcttgtcgg tggtcatgat cgtcgggatg tagggatagg
     3481 tggcggcgat gtagttgtag aggtcgaagg cgttgtcccc cggcagctcc acctcggaca
     3541 cgatgaggtc gaaatcgtag gcgcggagct tctgcatcgc ctcgaaggcc gattcgcaga
     3601 cctgcgtggc atagccggcc tcggtgaaga tcttctcgag cgtgatcttg tagacggaga
     3661 aatcgacaat cagaacctgg tacatgcctg cggtggtata tttggagaaa ccgctgtcaa
     3721 ggacaaaaaa aatcaaagat gatccgacgg cgcgcgtagt attaacatgg aaacccaaaa
     3781 cgattcaata ctcatggccg ttgcgatatc gattgtttcg tcgcctggac ggcgcgatct
     3841 ctggcgcgag atgcgcgcga cggagccggg cgaactctac gcgcgaatcg ccgccggtga
     3901 gccgccggcg gtgcaggcgc atgtggcccg ccagtacgcc ggaacgcccc tcgaagcggc
     3961 gaaacgcatt cgggagatgg ccgaacggcg tgacgtgcgc atcgtaagca tgtgggatga
     4021 ttcgtatccg tcgcttcttc gcgagatata ccgtccgccg ctcgtgctgt atgtccgcgg
     4081 cgatcccgca ttcgaccgga tgatcgcgat cgtcgggacg cgcgcggcgg acgccggatc
     4141 ggcgtcgatc gcccggctga tctcgacgga actgacggcg gcgggactgt gcgtggtgag
     4201 cggcatggcg gtgggcatcg accgggaggc gcatatgggg gcgctgctgc agggccgacg
     4261 gaccgtcggg gtgctcgcca acgggatcga catccggtac ccgcgacgca acgccgacat
     4321 ctaccaggcg atcatcggga cgccgggatg cgggctcgtc tcggagtacc cgccgggaat
     4381 catcgccgat cgctggacct ttgtccgacg caaccgcatc atcagcggcc tgtcgcatgg
     4441 cgtggtcgtg gtgaaggccg gcgacaagag cggggcgctc atcaccgcgg gatacgccgt
     4501 cgagcaaaac cgcgaggtct tcgcctgccc cggtggatcg ttcaatcccg attatgcggg
     4561 ctgccatcgt ctcattcgaa acggcgcgca tctcgtgtcc gatacgcgcg acattctcgc
     4621 ggagctgggc ccggtcgcgg gggacctgcg gaccgcgccg gcggcgtcgt gcgtgtgtgc
     4681 gctctctgcg gatcatgcag ccgcgaaggc ggacgaggcg acgggccgat gcgacgatac
     4741 cgcgtcgctc tcggacgtcg agcgggtcgt ggtcggactc gtgacgagcg gatcgtggga
     4801 gatcgacacg cttatccgca gctccgggtt tgcaacggac cgggtcaccg aggcgctcat
     4861 catgctcgag atggcgggct acatcgcgcg cgacggtacg cggatcagat tgctgcaaaa
     4921 ataaaatgtt cttgattatt atgtcgcatt atgtcattgt accgcgcgtg cggataaaaa
     4981 catcgcgatg gcaatcaagc gaacatttat agtcctctgg gcgacgcttc tcgttgtgtc
     5041 gctgtcgcag ctgtttccga tcccgccgta cggcgaggcc gtctactcgc tctatacctc
     5101 gggctatttc gtggagctgg accggcagtt tcgcgtcgtg acggacgcgt ttctgggcgt
     5161 caagatgatg agccggccgg cctatgcctg ggtgcttctc gacgacatcg agaaggcgca
     5221 cgggatccgg atccgcgtgt acaatgcgcg cggggtccgg gtcacggcgc cgggcgctcc
     5281 cgagggcggc gtcgatgtcg atgtccaaaa cgcgctcgcc gcgccgaagc cgtccgtcgc
     5341 ctcgtgggtg tcgaggggct cgtattacag cattcttccg ctcgcgaacg agggtcagtg
     5401 cggcatctgc cataccggca cgtatcggaa caacatgatc ggggccgtga ccttcaagcg
     5461 tcggtgcgat tcgcggacct tctacgcctt cgagcggatc gcagtgtttg tggggttctc
     5521 cctcgtgttc gccctccttt tgttcgcgac ggtccggtgg gacccccaga aaagcgttcg
     5581 ggaactcttt gttcgggttg acaaaaatga atagggtttt cctagtgttc gatttgcagc
     5641 ctaaccccgg ggagcggtat gagcaatagt aaggaaggaa aaacactggt catcgtcgag
     5701 tcgcctgcga aggcgaagac catcaataaa tatcttggga gctcgtacac catcctctcg
     5761 tcgatgggcc acattatcga tctccccaaa tcgcgactcg cggtggacat cgacaatgac
     5821 ttcgtccccg agtacatcac catccgcggg cgcgcgaaga tattgaacga gctcaagaag
     5881 aacgccgcga aatcgagtcg cgtgctcctc gcgaccgacc ccgaccgcga gggcgaggcc
     5941 atctcgtggc atctggcgaa cgcgctcacg ccaaaaaacg caaacatcca gcgcatcgag
     6001 ttcaacgaaa taaccgagca cgccgtgaag gaggcggtgc agcatccgcg ctccatc
//
LOCUS       DAORHF010000224         6054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13155_length_6054_cov_4.769128, whole genome shotgun sequence.
ACCESSION   DAORHF010000224 DAORHF010000000
VERSION     DAORHF010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13155_length_6054_cov_4.769128"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PKJ16_14980"
     CDS             <1..218
                     /locus_tag="PKJ16_14980"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HNV48346.1"
                     /translation="PAVMASQGMMTWVPTIEISVNVRAMPQSTWLRCSLRTRFITCGL
                     LEADGEVWDEAGNLVAISRQIAQAKKL"
     gene            237..1052
                     /locus_tag="PKJ16_14985"
     CDS             237..1052
                     /locus_tag="PKJ16_14985"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HNV48347.1"
                     /translation="MLILGVLLLALLPAHGRLAAAPNERARVIADIPAPPGHVRIPAK
                     MDSFAHWLRNRPLLPPGAPVYYFDGRRKPNQAVHYAVLDMDIGKRNLQQCADAIMRLR
                     AEYLYGRGLHERIVFSVTSGQPLSFREWAEGYRPRFGTRSMRMEKRAARDYSEGNLRS
                     YLVTLYAYAGTASLVRDLTHVRSLRDMRIGDVFIQGGHPGHAVIVVDMARDPSGGPGM
                     FMVAQSYMPAQSVHVLRNPADPDGGPWYRDDFGAQLVTPEWNFSAADLRRFRD"
     gene            complement(1056..1346)
                     /locus_tag="PKJ16_14990"
     CDS             complement(1056..1346)
                     /locus_tag="PKJ16_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48348.1"
                     /translation="MFSSYQLLGKTLDAVEARVSDRFEDLNSVFVLLDKYCTSVFASD
                     AKEKKGEDLSVPLEAALNISITLLKMIDSGSTGPDDRIAEELTKIRDKYFGR"
     gene            complement(1389..2306)
                     /locus_tag="PKJ16_14995"
     CDS             complement(1389..2306)
                     /locus_tag="PKJ16_14995"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HNV48349.1"
                     /translation="MSLVNAIGNTPLVELSNIWDARKTGVRMMAKLEGANPGGSIKDR
                     PAYYMIRKAIERGDLTKDRTILEPTSGNTGIGMAMIAAAMGYKIKLMMPACVSEERRS
                     VLMALGAELELTPGCDRTDGAITRAHHVMEMQGDRYYMPNQFDNDANWQAHYETTAPE
                     IVRDTKGEVDLVAAGMGTTGTLMGCSRYFRDHHPRTTVLGVEPTLNHRIQGLKNMSES
                     IVPGIYKPELIDRQLMCSDDNAFETVRDLSLIEGLFCGISSGAALWGAMQAARDLPRG
                     STVVVILPDRGDRYLSTEVFRSICAQCPP"
     gene            complement(2338..2769)
                     /locus_tag="PKJ16_15000"
     CDS             complement(2338..2769)
                     /locus_tag="PKJ16_15000"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HNV48350.1"
                     /translation="MEPFIWTSEYEIGIVDVDRQHRELFRLMDSLSLSLYRGDGKKVV
                     GQVLHSLEEYVAQHFSLEEDLMSRHRYPDVVRHMTQHRQFEKQLAAFKKDYERGGSDS
                     YLALRVNKELVAWWKTHILQVDREYRSYIVDAPGDGTPTGA"
     gene            complement(2881..3690)
                     /locus_tag="PKJ16_15005"
     CDS             complement(2881..3690)
                     /locus_tag="PKJ16_15005"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HNV48351.1"
                     /translation="MQEPFVVVLLEAEDGIHDDAPDSHYRDFRYGDSADDGGDRDVAS
                     AAWASLLADAEREANGLDGRFMPLPSVTAADAVSGAIYINPFLAFGDGRHPTTALCYR
                     FLHRHLSSVPEPIRRGMSLIDVGTGTGILSILAAKLGVDDIDAFDLSPAAVESAVENC
                     RMNECGRIRVRRDDLARLDPVRDYDIVLANIVSPIIIEHCAQLVRLMKPDGVMFASGV
                     SSLADTGVRECFAHNALRVIDHESAQGWNAYVLRKESGGPLSCDASSPFLP"
     gene            complement(4058..>6054)
                     /locus_tag="PKJ16_15010"
     CDS             complement(4058..>6054)
                     /locus_tag="PKJ16_15010"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48352.1"
                     /translation="SVFIAFALEFAARGNILRRPIVVIPLILFPLGFTLLQLDNRILG
                     YLLLKPYGWVGMWTESIWTALYLIYYLTATGIGLAIIWHFRTRTDDRQRKKQSLTLIV
                     SVLISLVAGTLTSVVLRLFNVYWIPPLGDVTVLAWTAGLFYATVRYRLFEFTPSAAAE
                     EILDTMTEALLLVGPTGSIVTVNAAATALLGAGSSADCVGRDILTLFPRDARDAIVRA
                     LYKTSFVSNLRSSIFSLDGREIPVLLSSTALSDEGGRVQGYVCVIRDFTDIKAAEERL
                     MEARDVAEQANRAKSTFLATMSHEMRTPLNAVLGYTRLLRADAHDVDTTARLEYIERS
                     GEQLLALINEMLDFAKIESGTMQLSAENFSLRAMLEDIERTFALRAEERRLGFSVCCA
                     EDVPATVRGAGVRLRQVVSNLVENAIKFTERGSVSVSCIYDSGAAFIEVVDTGPGIGA
                     DERQAIFEPFRQVDMSHTRAHGGIGLGLAICGRLVGLMGGVIEVGDGPEGGARFSVSV
                     PLTPLETPEVTADREAPVLQDLRAGTGVSAALDSGTCIALVADDNDLVRNLIGSILAR
                     LGVSHAFAENGAQAIEMLRRAPYRLLIMDIQMPEMDGMEAIRRIRADEALRDIYAIAL
                     TAHALEDDARRFLDAGFNDYISKPVDFPSFIGMVKRALGG"
BASE COUNT         1150 a         1966 c         1854 g         1084 t
ORIGIN      
        1 cgcctgcggt gatggcgagc caggggatga tgacgtgggt gcccacgatc gagatctcgg
       61 tgaacgtccg cgccatgccg caatcgacgt ggctgcggtg cagcctgcgt acgcgcttca
      121 ttacctgcgg cctgctggag gccgacgggg aggtctggga cgaggcgggc aacctggtcg
      181 ccatctcacg acagatcgcc caggcgaaaa agctctagga cccatcggtg cgcgtcgtgt
      241 tgatcctcgg agtactccta ttggcgcttc tcccggcgca cggacggctt gcggccgcac
      301 cgaacgagcg cgcgcgcgtc atcgccgaca ttcccgcgcc gccgggccat gtccggattc
      361 cggcgaagat ggactcattc gctcactggc tgcgaaaccg tccgctcctt ccccccggcg
      421 cgccggtgta ctacttcgac ggacggcgca agccgaatca agccgttcac tacgcggtgc
      481 tggacatgga catcggaaag cgcaacctgc agcagtgcgc cgacgccatc atgcgcctgc
      541 gcgcggaata cctgtacggc agggggctgc atgaacgcat cgtgttttcc gtgacctcgg
      601 gccagccgct ttcgtttcgg gaatgggcgg aagggtatcg cccgcggttc ggtacgcgct
      661 ccatgcgcat ggaaaagcgg gccgcgcgcg attactcaga gggcaatctt cgctcctatc
      721 ttgtgacgct ctacgcctac gcggggacgg cgtcgctcgt gcgggacctg acgcacgtgc
      781 gctcccttcg cgacatgcgc atcggcgacg tgttcatcca gggcgggcac ccgggacatg
      841 cggtgatcgt agtggacatg gcgcgggacc catccggcgg gccggggatg ttcatggtgg
      901 cgcaaagcta catgccggcg cagagcgtgc atgtgctgcg caatcccgcg gacccggacg
      961 gcgggccgtg gtatcgggac gatttcggcg cacaactcgt cacgccggaa tggaacttca
     1021 gcgccgccga tctgcggcgc tttcgcgact gatccctacc ggccgaagta cttgtcgcgg
     1081 atcttggtca gctcctccgc gatgcggtcg tcgggccccg tgctcccgct gtcgatcatc
     1141 ttgaggagcg tgatggagat attgagcgcc gcctcaagcg ggacggagag gtcctcgccc
     1201 tttttctcct ttgcgtcgct cgcgaagacc gatgtgcagt acttgtcgag caggacaaac
     1261 acgctgttga ggtcctcgaa acggtcgctc acccgcgcct caaccgcgtc cagggtcttt
     1321 cccagtagtt gatacgatga aaacatgtct cacctcggaa tgtgcaatat aacgtccctc
     1381 gtgcgcgctt agggcgggca ttgcgcgcag atggaccgga agacctcggt cgaaagatac
     1441 cggtcgccgc ggtcgggcaa gatgaccacc accgtcgatc cgcgcggaag gtcgcgcgcc
     1501 gcctgcatgg ccccccacag cgccgcgccg ctcgatatcc cgcagaacag cccctcgatg
     1561 agcgagagat cgcgcacggt ctcgaaggca ttgtcgtcgg agcacatgag ctggcggtcg
     1621 atgagttccg gcttgtagat ccccggcacg atcgattcgc tcatgttctt gagcccctgg
     1681 atgcgatgat tgagcgtggg ctcgaccccc aggaccgtgg tgcggggatg gtgatcgcgg
     1741 aaatagcgcg agcaccccat cagcgtgccc gtggtgccca ttccggccgc cacgaggtcc
     1801 acctccccct tcgtatcgcg cacgatctcc ggcgcggtgg tctcgtaatg cgcctgccag
     1861 ttcgcgtcat tgtcgaactg gttcggcatg tagtagcggt cgccctgcat ctccatcacg
     1921 tgatgcgcgc gggtgatggc gccgtcggtg cgatcgcatc cgggcgtgag ctcgagctcg
     1981 gcgcccagcg ccatgaggac ggagcgccgc tcctcgctca cgcaggcggg catcatgagc
     2041 ttgatcttgt agcccatggc cgcggcgatc atcgccatgc cgatcccggt gttcccggag
     2101 gttggctcca ggatggtccg gtccttcgtg agatcgcccc gctcgatcgc cttcctgatc
     2161 atgtagtacg ccggccggtc cttgatggag ccgcccgggt ttgcgccctc gagcttcgcc
     2221 atcatgcgca cgcccgtctt tctcgcgtcc cagatgttcg aaagctcgac cagcggcgta
     2281 ttgccgattg cgttcaccag gctcatggcc gaagcctccc gtcgcgaccc ggggcgatca
     2341 tgcgccggtc ggcgtaccgt cgccgggcgc atcgacaata tacgatcggt attcgcgatc
     2401 gacctgaaga atatgcgttt tccaccatgc caccagctcc ttgtttacgc gcaaggcgag
     2461 ataggaatcg gacccgcccc gctcgtagtc ctttttaaac gcggcgagct gtttttcaaa
     2521 ctgccggtgc tgcgtcatat ggcgcacgac atccggatac cggtgccggg acatcaggtc
     2581 ctcctcaagc gaaaagtgtt gcgccacgta ttcctccaac gagtgaagca cctggccgac
     2641 aaccttcttt ccatcgcccc gatacagcga caggctgagc gagtccatga gccgaaacag
     2701 ttcccgatgc tggcgatcga catctacgat gccgatctca tattcggaag tccagataaa
     2761 cggttccatc gtatcacccc gatcggttcc cggttcgcac cggcgctctc gagacaccgg
     2821 cacatgcgca catgcgggac ccgggaagcg caaaacacca taggcatgca cgcgtcactg
     2881 tcaaggcaaa aagggtgatg atgcatcgca tgagagcggg ccccccgact ccttccggag
     2941 cacataggcg ttccagccct gcgccgattc gtggtcgatc acacgaaggg cgttgtgcgc
     3001 gaaacactcg cgcacccccg tgtccgcgag cgaggatacg ccgcttgcga acatcacccc
     3061 gtccggcttc atgagccgca ccagctgcgc gcagtgttcg atgatgatcg gcgagacgat
     3121 gttcgccagc acgatgtcgt agtcgcgcac ggggtcgagc cgcgcaaggt cgtcccggcg
     3181 cacccggatg cgcccgcatt cgttcatccg gcagttctcg acggcgctct cgacggccgc
     3241 cgggctgagg tcgaatgcgt cgatatcgtc cacgcccagt ttcgcggcga ggatggagag
     3301 gatgcccgtt cccgtcccca cgtcgatgag ggacatgccg cgccggatcg gctccgggac
     3361 cgaggagagg tgccggtgca aaaagcggta acacagcgcc gtcgtggggt gccgcccgtc
     3421 cccgaaggcc agaaaggggt tgatgtagat ggcgcccgag accgcgtccg ccgccgtcac
     3481 ggatgggagc ggcatgaaac gaccgtcgag accgttcgcc tcgcgttcgg catcggcgag
     3541 cagagaggcc caggcggcgc tggcgacatc gcggtcaccg ccgtcatcgg cggaatcacc
     3601 ataccggaaa tcgcgatagt gcgaatccgg cgcatcatcg tgaatgccgt cctccgcttc
     3661 caataaaacg acaacaaacg gctcctgcat gcggcgaact ccgtcagaag atttttttgc
     3721 gaaagaaatc ccgaccgggg acatcgaaaa cgtccgcacc tccgcaattc acagttatat
     3781 acgcatgcgg gcggtcaaga agaatggctc cggcatcggc gcaaacggat gaaaaatctt
     3841 ttgatatctt tccgatgagc gctatgctca ttcacatcga agttgcatcg gagacgccat
     3901 gctaaaaaac gaggccgttc gttctacgct tttctttctc gccatcatgt tcatctggct
     3961 gtttgtcgcg ggctgggtgc ttccaaagtt cggcatccgc acctgactgg ccggcatctg
     4021 ttctcagttg tgagaaccga aaaacgatgc ggaacactca tcccccgagc gcccgcttca
     4081 ccataccgat aaacgaagga aagtccacgg gcttcgagat gtaatcgttg aacccggcgt
     4141 cgagaaaccg ccgcgcgtca tcctccaaag catgcgcggt gagcgcaatc gcgtaaatgt
     4201 cccgcagcgc ctcgtcggcg cgaatgcggc ggatcgcctc catcccgtcc atctcgggca
     4261 tctggatgtc catgatgagc aggcggtacg gggcgcgccg gagcatctcg atcgcctgcg
     4321 cgccgttctc ggcgaaggca tgggacacgc ccaggcgcgc caggatgctg ccgatgagat
     4381 tgcggacaag atcattgtcg tcggcgacga gcgcgatgca cgtcccggag tcgagcgcgg
     4441 ccgacacacc agttccggct cgcaggtcct ggagcacggg cgcttctcgg tccgcggtca
     4501 cttccggcgt ttcgaggggc gtgagcggaa ccgaaacgga aaaacgcgcc cccccctccg
     4561 gcccgtcgcc aacctcgatg acgccgccca tcaaccccac gagccgtccg cagattgcta
     4621 gccccagccc gatcccgcca tgggcgcggg tgtgcgacat gtcgacctgc cggaagggct
     4681 cgaaaatggc ctgccgttcg tccgccccga tccccggacc ggtgtccacc acctcgatga
     4741 acgccgcgcc cgaatcgtat atgcacgaca ccgagacgga accgcgttcg gtgaacttga
     4801 tcgcattctc gaccagattg ctcacgacct ggcggaggcg caccccggcc ccacgcaccg
     4861 ttgcgggaac gtcctccgcg cagcataccg aaaaacccag gcgacgctcc tcggccctga
     4921 gcgcaaaggt gcgctcgatg tcctcaagca tggcgcgcag ggaaaagttc tccgcggata
     4981 gctgcatcgt cccggattcg atcttggcga aatccagcat ctcgttgatg agcgcaagga
     5041 gttgctcgcc tgaccgctcg atgtattcga gccgcgccgt cgtatcgaca tcgtgcgcat
     5101 ccgcccgcag gagccgcgtg tatcccagaa ccgcattcag gggcgtacgc atctcgtggc
     5161 tcatggtggc cagaaacgtc gacttcgcgc ggttcgcctg ttcggcgaca tcgcgcgcct
     5221 ccatcaggcg ctcctcggcg gccttgatgt ccgtgaaatc gcggatcacg cacacgtacc
     5281 cctgaacgcg gccgccctca tcgctcagcg ccgtggagga caggagcacc ggtatttccc
     5341 ggccgtctaa ggagaatatc gacgaacgca aatttgagac aaagctcgtt ttgtacagcg
     5401 cccggacgat agcgtccctc gcgtcccgcg gaaaaagggt caggatgtcc cgaccgacac
     5461 aatccgccga cgaacccgcg cccaacagcg cggtcgccgc ggcattgacg gtgacgatgg
     5521 agccggtcgg tcccacaagc agaagcgcct cggtcatcgt atcgaggatc tcctctgcgg
     5581 cggccgaggg cgtaaactcg aagaggcgat aacggaccgt cgcgtaaaaa agcccggcgg
     5641 tccaggcaag caccgtcacg tcacccagcg gcgggatcca gtagacattg aacaggcgca
     5701 acaccacgct ggtgagcgtc cccgcgacca ggctaatcag cacagaaacg atcagcgtca
     5761 gcgattgttt ttttctctgg cggtcgtcag ttcgcgtccg gaaatgccag atgatcgcca
     5821 ggccgatccc cgtcgcagtc aggtaataaa tcaggtacag cgcggtccag atcgactcgg
     5881 tccacatccc cacccatccg taaggcttga gcagcaaata ccccaggatc ctgttgtcga
     5941 gttgcaggag ggtgaatccg agcgggaaca ggatgagcgg aatgaccacg atcggacgcc
     6001 gaaggatatt gccccgagcg gcgaattcga gcgcgaaggc gataaagacg ctgc
//
LOCUS       DAORHF010000225         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13300_length_6016_cov_5.694179, whole genome shotgun sequence.
ACCESSION   DAORHF010000225 DAORHF010000000
VERSION     DAORHF010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13300_length_6016_cov_5.694179"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PKJ16_15015"
     CDS             <1..350
                     /locus_tag="PKJ16_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HNV48353.1"
                     /translation="FRGGVVAYGNEAKIEILDVSAATLQRCGAVSEEVAGEMAAAARK
                     RFGTDIGISASGVAGPGGGTDEKPVGMVCFGFASPKGVDTFTRYIKMDRERVRFYASQ
                     YILDKVRLHFKNG"
     gene            355..3186
                     /locus_tag="PKJ16_15020"
     CDS             355..3186
                     /locus_tag="PKJ16_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48354.1"
                     /translation="MRPFPVAIALAVVLSLCPRAGAADDLPSLFEQGLKAYETRSYAA
                     AELIFGTIVESKNDVYRDRAWFHLALSIYHQGRYSAALFELNRFLLMCQTQTLAVRAQ
                     FWVAECLRQMKRALPAIEEYKRFIARYPGDDLAVVARERIGDIYFSEGRYDEAVIEWE
                     RVIPMNADRSRRLRIAVKIGEAQFWSGRDARALETLAPALDSMNDARLEARARIVAGR
                     AYQRQNRHQDAIRQFSRVPSALAREAPYSDAQYFIALSYYAEGDARRAWALLEEFIGI
                     GLRSDWFFDALYMLGRINIEEGSEAEGMRLLEQVRSGTGKPELKARAALLLSRIYLRK
                     NPRQAIPYLEDVTSIPDPAEQKNALMLLGRAYMAERRYDNAERTLDLAESRFPYDPVV
                     DEIRFLKAGIAIEKGDFERGISALDDIARTNPFSRFINESLYYRALADHRRGAHATAR
                     TRLQRYLGGAHPERGFAATALLIEVHVAQKNYPEAERTALSLAARFPDHAGLDSALCA
                     LARRLSAAGRDGERYYRLVMERYPASPCACVQARERGDRAIDRREFALAEEQYGRFLK
                     HCAEETSGEVFAWWAQSIYRQGRFAEVAALGVHERFQASEGPARSALAILIAQSMYRQ
                     GDYGGAHRYFTIVGREGLRDDERHEYVIACAAVEDFEGLGGALATLERNKPLYRRSLS
                     ELGRFFRGAGNVEMAKTYYQWYLLEEPAKEPSDTVRLILAEIGAEQGDYASAIVQLND
                     ITTPSLLQRQLSLLIVAYFRSGNIASAVALSERYGADLVASRAGEAAVRENVQYYFHQ
                     DDWDGFERYALLLRRFPGNEAYLEYHEGLLALRLGEYPRARRIFERLGDGQSNYRDEA
                     VFLSGMIAQYAFQDVAGAMKIFNRLTGGAGSTTVLADRARIHLAVLAFEAGRPEESRR
                     YLDAVLASSKSDMDRTRARNLLEYFRLAP"
     gene            3196..3651
                     /gene="aroQ"
                     /locus_tag="PKJ16_15025"
     CDS             3196..3651
                     /gene="aroQ"
                     /locus_tag="PKJ16_15025"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HNV48355.1"
                     /translation="MDHTKTILVINGPNLNLLGMREPNTYGTKSLDEILEAMRAAADR
                     HGVLLNFFQSNSEGAIIDFLHERTGADGIIINPAAYTHTSIAIRDAIAALGAPAVEVH
                     LSNVHAREEFRRHSLIAPVCIGQIAGFGPHSYILALDAIVAHIARSRAE"
     gene            3656..5236
                     /locus_tag="PKJ16_15030"
     CDS             3656..5236
                     /locus_tag="PKJ16_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48356.1"
                     /translation="MPVRGRLFQSTVLVGVFATFLACGACKERNQRESEPNDTFANAN
                     PITAHMTVEGALERTNDRDHFLLTVTEPLVLDISLSGVKGVNQAFQVWRRTGESYTLL
                     KHVDDQRKSSPERLCNLYVLPGTYCIVVLHGDKDAPQTNRETPYRLKVASRSIKDYEE
                     QEPNDSPETANPIPMPGEIFGFFSPAINKHNTNKEHPRREEDWFSFEVSLNDNKPILV
                     DIELSGVPAVNSTLQIFSGDMEELGRADVGEVGKGESIRGVGITTSGRYYLMVAARNV
                     EANNDIPYSVKITKREYNYSLEMEPNNTLERANLIAENQVSGMIFPRGDIDWYRVQGG
                     DELQVYRVEAVPPPDVDVQLRLYNDRRERLWETDQAKRGERAIMPGIVLRGEFFIEVY
                     SKTGAVNQDKPYLLTLVTKTYADGWEKEPNDTKAAATKVTGKTVLGFTSKRKDVDYFL
                     FEYNRRVRCRYVIRAAQGSELQVSATDPMGYILKTVAAVGGKSVRLDEMVDNRGYLVV
                     QTSIENLDEPYVIEVETE"
     gene            5233..5709
                     /locus_tag="PKJ16_15035"
     CDS             5233..5709
                     /locus_tag="PKJ16_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48357.1"
                     /translation="MMPMRRCAALLLIVVAMACCGSCAARRGDGAVDRKHTTPVRSDS
                     DLVVLVHDEGIADPARDRRCYYRVFVNKVEAGRTGIGLESQRKEFRTVVDRNRHLVSI
                     EKWVLDERKGRYEKLNNIEQPKPNFLYVTAGNDRIIVRFVVDHETRRAVVTAGRSE"
     gene            complement(5797..>6016)
                     /locus_tag="PKJ16_15040"
     CDS             complement(5797..>6016)
                     /locus_tag="PKJ16_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48358.1"
                     /translation="VKRANMPYEKYIHEQIRAVHDAGASGFILWNASQDYSIPCRVSR
                     NYYTKGSSEIVSNDGPVSVDEGASKTTL"
BASE COUNT         1094 a         1855 c         1980 g         1087 t
ORIGIN      
        1 tgtttcgcgg cggcgttgtc gcttatggca acgaggccaa gatcgagatc ctggatgtga
       61 gcgccgcgac cctgcagcgc tgcggggcgg tgagcgagga ggtcgccggg gagatggccg
      121 ctgcggcacg gaaacgcttc gggaccgata tcggcatctc cgccagcggc gtcgccggac
      181 ccggcggcgg aaccgacgag aagccggtgg gcatggtctg cttcgggttc gcctcgccca
      241 agggcgtgga taccttcacg cgatacatca agatggaccg cgagcgggtc cgcttttacg
      301 catcgcagta tatcctggac aaggttcgcc tgcacttcaa gaacggctga tccgatgcgt
      361 ccgttccccg tcgccatcgc ccttgccgtt gtcctgtcgc tgtgccctcg cgccggcgcc
      421 gccgacgacc tcccgtcgct cttcgagcag gggctcaagg cctatgagac caggagttac
      481 gcggcggcgg agctcatctt cgggacgatc gtcgaatcga agaacgatgt ctatcgcgac
      541 cgcgcgtggt tccatctcgc cctgtcgatc tatcaccagg gccgctactc cgcggcgctc
      601 ttcgaattga accgcttttt gctcatgtgc cagacgcaga cgctcgccgt tcgcgcccag
      661 ttctgggtgg ccgaatgcct gcggcagatg aagcgcgccc tgccggccat cgaggagtac
      721 aagcgcttca tcgcgcgcta tccgggcgac gatctggcgg tggtcgcccg ggagcgcatc
      781 ggcgacatct atttttccga ggggcggtac gacgaggccg tgatcgaatg ggagcgcgtc
      841 atcccgatga acgcggaccg ctcgcgccgg ctccggatcg cggtgaagat cggcgaggcg
      901 cagttctgga gcgggcgcga tgcgcgcgcc ctggagacgc ttgcgccggc gctggattcg
      961 atgaacgacg cgcgactcga ggcgcgagcg cgcatcgtgg ccgggcgcgc ataccagcgg
     1021 cagaaccgcc atcaggacgc gatccgccag ttctcccggg tgccgtccgc gctggcccgc
     1081 gaggccccct attcggacgc acagtatttc atagcgttgt cgtattatgc ggagggggac
     1141 gcgcggcggg cctgggcgct cctcgaggag tttatcggca ttggcctgcg ctcggactgg
     1201 tttttcgacg ccctgtacat gctggggcga atcaacatcg aggagggtag cgaggccgag
     1261 gggatgcggc tcctcgagca ggtccgcagc gggaccggca agcccgagct caaggcccgc
     1321 gcggcgcttc tcctgagccg gatctatctt cgaaaaaacc cgcggcaggc catcccgtac
     1381 ctcgaggacg tgacgtccat tcccgacccg gccgagcaga agaacgccct catgctgctg
     1441 gggagggcgt acatggccga gcgccgctac gacaatgcgg aacggacgct cgatctggcc
     1501 gagagccgtt tcccgtacga tccggtggtg gacgagattc gattcctgaa ggcgggcatc
     1561 gccatcgaga agggcgattt cgagcgcggg atttcggccc tcgacgatat cgcgcggacg
     1621 aacccgtttt cgcgctttat caacgaatcg ctgtattatc gcgcgctcgc cgatcaccgg
     1681 cgtggcgccc atgccacggc gcgaacgcgc ctgcagcgct atctgggcgg cgcacacccc
     1741 gagcgcggct tcgcggcgac ggcgctcctg atcgaggtgc atgtcgcgca aaaaaattac
     1801 cccgaagccg aacgaaccgc tctgtcgctg gcggcgcgct tccccgacca tgccggtctc
     1861 gacagtgcgc tgtgcgcgct tgcgcgacgc ctgtccgcgg ccggtcgcga cggcgaacgc
     1921 tattaccgac tcgtgatgga gcgctatccc gcctctccct gcgcgtgcgt gcaggcgcgc
     1981 gagcggggcg atcgcgccat cgaccggcgt gaattcgctc tggccgagga gcagtatggc
     2041 cgcttcctga aacactgcgc ggaggagacg tccggcgagg tgttcgcctg gtgggcccag
     2101 tcgatctatc ggcaggggcg cttcgcagag gtcgcggcgc tgggggtgca cgagcgcttc
     2161 caggcgagcg agggcccggc gcgcagcgcg ctcgccattc tcatcgcgca gagcatgtat
     2221 cgccagggcg attacggagg cgcgcatcgc tatttcacca tcgtgggacg cgaagggctt
     2281 cgggacgacg agcggcacga gtacgtgatc gcctgcgcag cggtggagga cttcgagggc
     2341 ctcgggggcg cgctcgcgac ccttgaacgg aacaagccgc tgtatcgccg gtcgctcagt
     2401 gagctcgggc gctttttccg gggagcgggc aatgtcgaga tggccaagac ctattaccag
     2461 tggtacctgc tggaagagcc ggcaaaggag ccgtccgaca cggtccggct gattcttgcg
     2521 gagatcggcg cggagcaggg ggattacgca tccgccatcg tgcagctaaa cgacatcacc
     2581 acgccgtcgc tccttcagcg ccagctgtcc ctgcttatcg tggcctactt tcgctccggt
     2641 aacatcgcat cggcggtcgc gctcagcgaa cgctacggcg ccgatctcgt ggcgtcgcgg
     2701 gccggggagg ccgccgtccg tgagaatgtg cagtattatt ttcaccagga cgattgggac
     2761 ggtttcgagc ggtacgccct gctgcttcgc cggtttcccg gaaacgaggc gtacctcgag
     2821 tatcacgagg ggctgctcgc cctccgtctc ggggaatatc cccgcgcgcg acgcatcttt
     2881 gagcgcctcg gcgacgggca atcgaactat cgggacgagg ccgttttttt aagcgggatg
     2941 atcgcgcagt acgccttcca ggatgtggcc ggcgccatga aaatttttaa tcggttgaca
     3001 ggaggggcgg gttccactac tgttctggcc gatcgcgcgc gcatccatct cgccgtgctc
     3061 gccttcgagg ccgggaggcc cgaagagtcg cggcgatatc tcgacgcggt gctcgcctcg
     3121 tcgaagagcg acatggaccg gacgcgcgcg cgcaatttgc tcgaatactt caggcttgca
     3181 ccataacaac ggtcaatgga tcacacgaaa acgatactgg tgataaacgg ccccaacctc
     3241 aatctgctgg ggatgcggga gccgaatacc tacggcacga aatcgctcga tgagattctg
     3301 gaggcgatgc gcgccgccgc cgaccggcat ggcgtgctcc tcaatttctt tcagtcgaac
     3361 tcggaggggg ccatcatcga ttttctccac gagcggaccg gcgccgacgg catcatcatc
     3421 aatccggccg cctacaccca tacgtcgatc gccatccggg acgcgatcgc ggcgctcggc
     3481 gcgcccgcag tcgaggtgca cctgagcaac gtgcatgcgc gcgaggagtt tcgacggcat
     3541 tcgctcatcg ctccggtgtg catcggccag atcgccgggt tcgggcccca ctcgtatatc
     3601 ctcgcgctcg acgcgatcgt cgcgcatatc gcgcggtccc gtgcggagtg acggcatgcc
     3661 ggtgcggggt cgcctgtttc aatcgacagt gcttgtaggc gtcttcgcga cgttcctcgc
     3721 ctgcggcgcc tgcaaggagc gaaaccagcg cgagagcgag cccaacgaca ccttcgccaa
     3781 cgcaaatccg atcacggcgc acatgaccgt cgagggcgcg ctcgagcgga cgaacgacag
     3841 ggaccatttt ctgctcacgg tcacggagcc gctggttttg gacatctccc tgtcgggcgt
     3901 gaaaggagta aaccaggcat tccaggtatg gcggcgcacc ggcgaaagct atacgctcct
     3961 gaagcatgtc gatgaccagc gcaaatcatc ccccgagcgc ttgtgcaatc tctacgtttt
     4021 gcccgggacc tattgcatcg tcgtgctgca cggcgacaag gacgcaccgc agaccaaccg
     4081 tgaaacgccg taccgtctca aggtcgcgtc gcgctccatc aaggattacg aggagcagga
     4141 gcccaacgat tcgcccgaaa cggcaaatcc gatccccatg ccgggcgaga tcttcggttt
     4201 tttctctccg gccatcaata agcacaacac caacaaggag catccccgcc gcgaggagga
     4261 ctggttctcg ttcgaggttt ccctcaacga caacaagccc attttggtcg atatcgagct
     4321 ctcgggcgtt ccggccgtca attccacgct gcagatattc tccggggaca tggaagagct
     4381 cggccgtgcc gatgtgggcg aggtcgggaa gggggagagc attcgcgggg tgggcatcac
     4441 cacgtcaggc cggtattatc tcatggtggc cgcacgcaac gtcgaggcca acaacgacat
     4501 tccctatagc gttaaaatca cgaaacgcga gtacaattac tccctggaaa tggaaccgaa
     4561 caacaccctg gagcgcgcca atctcattgc cgagaaccag gtttccggca tgatctttcc
     4621 gcgcggcgat atcgactggt atcgcgtgca gggcggcgac gagcttcagg tctaccgcgt
     4681 cgaggcggtg ccgccgcccg atgtcgacgt gcagctgcgt ctctacaacg atcgccggga
     4741 gcggctgtgg gaaaccgacc aggccaagcg cggcgaacgc gccatcatgc ccggcatcgt
     4801 gttgcggggc gagttcttca tcgaggtgta ctcgaaaacg ggagcggtca atcaggataa
     4861 gccgtatctg ctcacgctgg tgaccaagac gtacgccgat ggctgggaga aggagccaaa
     4921 cgacacgaag gccgcggcca cgaaggtcac ggggaaaacc gtgctggggt tcacctcgaa
     4981 gcggaaggat gtggattatt tccttttcga gtacaatcgg cgtgtgcgct gccgatatgt
     5041 tatccgggcc gcgcaggggt ccgagctcca ggtgtcggcg acggacccca tgggttatat
     5101 cctcaagacg gttgcggccg tcggcggaaa atcggtgcgg ctcgacgaga tggtcgacaa
     5161 tcggggctac ctcgtggtgc agacctcgat cgaaaatctc gacgagccct atgtgattga
     5221 ggtggaaacg gaatgatgcc gatgcgacgt tgtgcggcgc tcctgctgat cgtcgtcgcc
     5281 atggcatgct gtggctcgtg cgcagcgcgg cggggcgatg gcgccgtgga ccgaaagcac
     5341 accacgcccg tgcgcagcga cagcgacctc gtcgtcctcg ttcacgacga gggcatcgcc
     5401 gatcccgcgc gcgaccggcg gtgctactac cgcgtatttg tcaacaaggt cgaggcggga
     5461 cggacgggga tcggcctgga gtctcagcgc aaggaattcc gcacggtcgt tgaccgcaac
     5521 cggcacctgg tgagcattga aaagtgggtg ctcgatgaac ggaagggacg atacgagaag
     5581 ctgaacaaca tcgagcagcc gaagccgaat tttctgtatg ttaccgccgg taatgatcgg
     5641 atcatcgtcc ggttcgttgt ggatcatgaa accaggcgcg cggtcgttac cgcgggccgg
     5701 agcgagtagc ggacgacgcg cattgtggtg ggacgataaa ggcggacccg gtggggtccg
     5761 cctttgtgcg cgatggtgtc gcgtcgatcg acgccgttag agggtggtct tcgacgcgcc
     5821 ctcatcgacg ctcacggggc cgtcattgga aacgatctcc gaggagcctt tggtgtaata
     5881 gttccgggag accctgcagg gaatgctgta gtcctgcgag gcgttccaga ggatgaagcc
     5941 gctcgcgccg gcgtcgtgca cggcccggat ctgctcgtgg atgtatttct cgtacggcat
     6001 gttcgcccgt ttcacc
//
LOCUS       DAORHF010000226         5988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13396_length_5988_cov_5.104163, whole genome shotgun sequence.
ACCESSION   DAORHF010000226 DAORHF010000000
VERSION     DAORHF010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13396_length_5988_cov_5.104163"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="PKJ16_15045"
     CDS             <1..535
                     /locus_tag="PKJ16_15045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HNV48359.1"
                     /translation="FMFERSRVISTQFVRRGSSHSIEVALIGEPISAEMMDHLKKILP
                     QYDLEKTELKIIQPTEIGRGDDKKHADARMEMMASVKSKDDQIRLLESELQLLKSGDR
                     RVQKATKEANILFPSLESISFGDLLVHNVQDLSSLREVAVIVKWKAFAAAADKKRLEL
                     FLKSRLEIENLKVLEGK"
     gene            854..2017
                     /locus_tag="PKJ16_15050"
     CDS             854..2017
                     /locus_tag="PKJ16_15050"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HNV48360.1"
                     /translation="MKNLRAKCFMFGCVILCIIVAAVAFSDDFSSQPQKALIMAIVAH
                     PDDEPSALLACYGGGLGLPVIWVQMTSGDAWCGGKIPAGPKCGAVREKESLDAARNYG
                     MKNPPIFGRYVDCCGWKKDRLAENLERWGGKEKALAFVVGLIRKHRPEVVWTHNVDGE
                     YRHNNHIACSLITREAVLAAADPSRFRDLPEANDPWKVKKLYLYNHGWNKWYKMDAIL
                     PGLGGRSVAKAAADGYHCHVTQGKDKETPGSGVRGGLWSSSVGPDVAGDDILENLDLA
                     RWKPRTIYGTVSLPAGQVAPEGGIRLTMWIVDETNKSWRRFAHIPAGKNGTWFHLLYG
                     MDTGKSYRVGVQRDLPGQKKKLFWHRDGMKPEKEADRVTLEKDGAKIDLVAPE"
     gene            2498..3985
                     /locus_tag="PKJ16_15055"
     CDS             2498..3985
                     /locus_tag="PKJ16_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48361.1"
                     /translation="MNMRLAIAVTISLALLCAMPGAAAGETGRGAYDAGKVKLDAGDF
                     SGAADDFARACQLEPGNRTFLLWHMRLQVFLGQNAAFSGDHRQAITLFSQAMAAKEKL
                     TTAKDSEYLAIRRLLDLTREHQSLGSIKPEYVHRVKVLYIRHTDLQGVVDKDGRPVTG
                     KGSMSDSQITASIRAQDQLRFFIEALTRGKLSLSFDRTVVDATLTKAVIYARQANTTM
                     YRPDFSSTSESLGPYLFPKRNNIDSIFMYFINPGFSVWPFANAVPLIYVPYTWYGPER
                     GSIALPMDAAVAKNNTELVTNFPHSWVTFHEFFHVIERRSGGIGPMHGWLPESIEKSK
                     KAYPDWVPDANATWTATEYTWYRYHLLNTLPARMQMQSQKSGITPPFANFSFSLTSPD
                     GHNDAIFKTYTRVLAKISLENLRLAEELVAQADKARRSKRTEEARALYTKALNHNQYH
                     HRALYELGMDAHRRRDSAEAERYFSILERVFPDPKYARPTSSPTR"
     gene            complement(4157..4227)
                     /locus_tag="PKJ16_15060"
     tRNA            complement(4157..4227)
                     /locus_tag="PKJ16_15060"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4193..4195),aa:Gly,seq:tcc)
     gene            4480..4917
                     /gene="mraZ"
                     /locus_tag="PKJ16_15065"
     CDS             4480..4917
                     /gene="mraZ"
                     /locus_tag="PKJ16_15065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HNV48362.1"
                     /translation="MFMGEYHPTLDDKGRVAIPARLRKAFGEQAMINRLVITHGFDKC
                     IMAFREEDWKEFVAQKLVPLSQADPKNRMRLRFLMGGAAECDLDKQGRIGIPGHLQEY
                     AQISREVVILGLFDRLEIWAEDVYREYKPDGAALDSFAHELGF"
     gene            4986..5891
                     /gene="rsmH"
                     /locus_tag="PKJ16_15070"
     CDS             4986..5891
                     /gene="rsmH"
                     /locus_tag="PKJ16_15070"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HNV48363.1"
                     /translation="MHDPVLCAEIVALARETRIDRGGIFVDATLGEGGHSALILASFP
                     AARVIGIERDPEILARARERLHGAAGRIEFINDNFANIAGALGAHRGKIAAVLYDFGI
                     SSFHYDASGRGFSFRDHEPLDMRLDGARGESAADIVNARPERELADIFRVYGEERWAK
                     KIARRIVERREDGRIETSLALAELVLGAIPRRFHVKNIHPATRVFQALRIIVNDELSA
                     IERSLADALDLIEPGGRIIAISFHSLEDRIVKTMFRARAKVPYGEEPSFRLITKKPIV
                     PGDDEVARNRRARSAKLRCIERLAS"
BASE COUNT         1317 a         1575 c         1797 g         1299 t
ORIGIN      
        1 cttcatgttc gagcggagcc gggtgatctc gacgcagttc gtgcgtcgcg gcagctcgca
       61 ttcaatcgag gtcgccctga tcggggaacc gatttcggcg gaaatgatgg atcatttgaa
      121 gaagatcctg ccgcagtacg atctcgagaa aaccgagctc aagatcattc aacccacgga
      181 gatcggccgc ggcgacgaca agaaacatgc cgacgcgcgc atggaaatga tggcgagcgt
      241 caagtccaag gacgaccaga tccgcttgct cgagtcggag cttcagcttc tcaagagcgg
      301 agatcggagg gtccaaaagg cgacgaagga ggcgaatatt ctattcccgt cgcttgagtc
      361 gatctccttc ggcgaccttc tggttcacaa tgtacaggac ctctcctctc tcagggaggt
      421 cgcggtcatc gtcaaatgga aggcgttcgc cgcggccgcg gacaagaagc gcctggagct
      481 gttcctgaaa tcacgtctgg agatcgagaa cttgaaggtg ctggagggga agtgagaggc
      541 cagatgagcg agtgatggga ctcgaaccca cgctatcagc ttggacgttt caattcccac
      601 cttgcgatta gcgcactaat gcgtttgcca tgaccattgt ctgagtgaat gccaggcgaa
      661 ttgttgggaa atgggtaacc atgggtcggg gtaggtgttt tataaaaagc cgcgtgatat
      721 tgcccgcggt tattacgaca attaaagagt ttgcaggtct tgggagtctg cgtgtcggcg
      781 ctgcgtcttt caggattgat tggttttcct gcttgactaa actcgttgta tacagttgaa
      841 tatgttagtg aaaatgaaaa atctacgagc aaaatgtttt atgttcggct gcgtcattct
      901 ttgtattata gttgcagccg ttgctttttc tgacgatttc tcatcgcagc cgcagaaggc
      961 attgattatg gccatcgtgg cgcatcccga cgacgagccg agcgcactgc tcgcatgcta
     1021 tggcggcggt ttggggcttc ccgttatctg ggtgcagatg acaagcggcg acgcatggtg
     1081 cggcggaaaa atacccgccg gtcccaagtg cggcgccgtc cgcgaaaagg agagtcttga
     1141 cgcagcacga aattatggca tgaagaatcc accgattttc ggccgctatg tcgactgctg
     1201 cggctggaaa aaggatcggc ttgccgagaa ccttgagcga tggggcggaa aagaaaaagc
     1261 gctcgctttt gttgttggac ttatccggaa acaccggccc gaggtcgtct ggacgcacaa
     1321 tgtcgacggt gaatacaggc acaacaacca tatcgcctgt tcgttaatca cgcgtgaagc
     1381 ggtactggcc gccgccgacc cgtcacgctt ccgcgatctt cccgaagcaa atgatccatg
     1441 gaaagtcaag aagctctact tgtacaatca tggatggaac aagtggtata aaatggatgc
     1501 aatacttcct gggcttgggg ggcgttccgt ggcgaaggcg gccgcggacg gatatcattg
     1561 ccatgtcacg caaggcaagg acaaggagac acccggatcg ggagttcggg gcggactctg
     1621 gagcagctcg gttgggcccg atgtcgccgg cgacgatatt ctcgaaaatc tggacctggc
     1681 gcgctggaaa ccgcgtacca tttacggcac cgtctcgctg cccgcaggac aagtcgcccc
     1741 ggagggcggc attcgtctga ccatgtggat cgtcgatgag acgaacaagt catggcgacg
     1801 attcgcccat atacccgccg gaaagaacgg cacgtggttt catctacttt acggcatgga
     1861 tacagggaag tcatatcgtg tcggggtgca gcgggatctg cccggacaga aaaagaaatt
     1921 gttctggcat cgcgatggca tgaagcccga gaaagaagcc gaccgcgtca cattggagaa
     1981 agacggcgcg aagatcgacc tggtcgcgcc ggaataatac atcaccctca tttcgggaaa
     2041 ggccgcgcat ttccacatgg tgaaatgggt gacccggttt gcgtaacggt tttccgcaaa
     2101 cacggtatag gcgtaatttc tgtattgctg cggttgttgc gcttctcaat catcggcgga
     2161 ctctcttgcc cgaaaatgca aaatcctgat ctatgccatc tgcacttgtc agatttcaga
     2221 tccacgtaaa gggttttttc atgtccatgc tgatgattac gggatgaacc catttccaat
     2281 aatcgtcggc atgtatcttg atacgttgcg aaaatgggcg gagctttcct aattgtaaaa
     2341 aacccagtca tcaaggtggt ttttccaaac cgtatcaatg cgtctggatg attataacga
     2401 gtcggataag aatcggatag ttcttgacgt ggaaaggaaa accgcgtatg acacgaccca
     2461 taagggtatt gtttgtgctc gattctggag gatgtgaatg aacatgagac tggctattgc
     2521 ggtaaccatt tctcttgcgc ttctgtgtgc catgccggga gccgctgccg gagagactgg
     2581 tcggggtgct tatgacgccg gcaaggtgaa acttgatgcg ggggattttt ctggggcggc
     2641 tgatgatttt gcgcgcgcct gccagctcga gcccggcaac cggacctttt tgttgtggca
     2701 catgcggctt caggtgttcc tggggcaaaa cgcggctttt tcgggagatc accgtcaagc
     2761 catcactctg ttttcgcagg ctatggccgc taaggaaaaa ctgaccacgg caaaggatag
     2821 cgagtacctt gccatccggc gcttgctgga tctgacacgc gaacatcaat ctcttggctc
     2881 gatcaagcct gagtatgtcc atcgggtcaa ggtgctctac attcggcata cagaccttca
     2941 gggggtcgtc gacaaggacg gcaggcccgt gaccggcaag ggaagcatga gtgacagtca
     3001 gatcacggcc agcattcgcg cccaggacca gctcaggttt ttcatcgaag cccttacccg
     3061 gggcaagctc agcctttcgt tcgaccgaac ggtcgtcgat gcaaccctga ccaaggctgt
     3121 gatttatgcc cgtcaggcca atacaaccat gtaccgcccc gatttttcaa gcaccagcga
     3181 aagtctcggg ccgtaccttt tcccgaaacg aaacaacatc gacagcatct tcatgtactt
     3241 catcaatccc ggattttcgg tctggccctt tgccaacgct gttcctctga tttacgtccc
     3301 gtacacctgg tacgggcccg aaaggggctc gatagccctg ccaatggacg ccgcagttgc
     3361 aaaaaacaat accgagctgg tgaccaactt tcctcattcc tgggtcacct tccatgagtt
     3421 ctttcacgta atcgaacgcc ggagcggagg gattgggccg atgcacggct ggctgcctga
     3481 atcaatcgaa aagtcgaaga aggcataccc ggactgggtg ccggacgcca acgccacctg
     3541 gacggcaacc gaatatacat ggtatcgtta tcatcttcta aacacgctgc cggcccggat
     3601 gcagatgcag tcccaaaaaa gcgggatcac gcctcctttt gctaacttta gtttttcgtt
     3661 gaccagtcct gacggccata atgatgcaat ttttaaaacc tatacccgcg ttctcgccaa
     3721 gatcagcctc gaaaacctac gcctcgcgga agagcttgtt gcgcaggccg acaaggccag
     3781 gcggagcaag cggaccgagg aggcccgggc cctgtacacg aaggcgctca accacaatca
     3841 gtatcatcat cgggcactct atgaactcgg gatggacgcg caccgcagac gggatagtgc
     3901 agaggctgaa cggtattttt cgattctcga acgggttttc cccgatccga aatatgcccg
     3961 gccgacatct tcgccgacac ggtagcaggg acgagtgagg atgaaacggg gtgtttaaaa
     4021 aaatatcggt ccgcatgaga caccaagagc gagacggtga gaatttcttc tcattttctt
     4081 cttattcttg atgatcgaga agcgctggtt ttcggtagct tgttggtaag tcctgatccc
     4141 atcaggactt acgatgagcg ggtgatggga atcgaaccca cgctatcagc ttggaaggct
     4201 gaagttctac cattgaacta cacccgcgcg ttgccataca ctctgagtat ggcaacgtat
     4261 ccgtcaagta tttttgcatc aatgccggac gtcgatggta tgtttgtacg ctgatgagtc
     4321 agccggacgg catcatcacc cctgaagaat aaaaatgtac atttcccaat aaaaaatcgt
     4381 tttattcttg acaattatgt ctcatggtgt agccctgtgg ggagaagtgg ggattcgtgt
     4441 aacaaccttc cagtttgtgc ggatttttgg ggcattgcca tgtttatggg tgaatatcac
     4501 ccgacgctcg acgacaaggg ccgcgtggcc attccggcca ggctgcgcaa ggcgttcggc
     4561 gagcaggcca tgatcaaccg gctcgtgatc acgcacgggt tcgacaagtg catcatggcc
     4621 ttccgcgaag aggactggaa ggagttcgtt gcccagaagc tggtgccgct ttcgcaggcc
     4681 gatccgaaga accgcatgcg cctgcgcttt cttatgggag gggctgcgga gtgcgatctg
     4741 gacaaacagg gacgcatcgg gatccccggg catctgcagg agtacgcgca gatatcgcgc
     4801 gaggtggtga tcctggggct gttcgaccgg ctcgagatct gggccgagga cgtgtaccgg
     4861 gagtacaagc cggacggcgc cgcactcgat tcgtttgcgc acgagctcgg gttttagcgc
     4921 aggcatgagc ggcgaagggg cgggttctaa tgagacataa tgctggtgag gcggattttt
     4981 ctcccgtgca cgatccggtt ctgtgcgccg agatcgtcgc gctcgcgcgc gagacgcgga
     5041 tcgatcgcgg cggaattttt gttgacgcta ccctgggcga gggcggtcat agcgcattaa
     5101 ttctcgcatc gtttccggcg gcgcgggtga ttgggatcga gcgcgatccc gagatcctgg
     5161 cgcgggcgcg tgaacgcctg cacggcgcgg ccgggcggat tgaattcatc aatgacaatt
     5221 tcgcgaacat cgccggggcg ctgggcgcgc atcgggggaa gattgcggcg gtcctctacg
     5281 atttcggcat ctcatcgttc cactacgacg cgagcgggcg gggcttcagc ttccgcgacc
     5341 acgagccgct cgacatgcgg ctcgacggcg cgcgcggcga gagcgcggcg gacatcgtga
     5401 acgcccggcc ggagcgcgag ctcgcggaca tcttccgcgt gtacggcgag gagcgctggg
     5461 cgaagaagat cgcgcggcgc atcgtcgagc ggcgagaaga cgggcggatc gagacgagcc
     5521 tcgcgcttgc ggagctcgtc ctcggggcga tcccgcggcg cttccacgtg aagaacatcc
     5581 acccggcgac gcgcgtcttc caggcgctgc gcatcatcgt gaacgacgag ctttcggcca
     5641 tcgagcgctc gcttgccgac gcgctggacc tcatcgagcc cggcgggcgc atcatcgcga
     5701 tctcgtttca ttcgctcgag gaccgtatcg tgaaaaccat gttccgcgcg cgggcgaagg
     5761 tcccctacgg cgaggagccg agcttccggc tgatcaccaa gaagcccatc gtgccgggcg
     5821 atgacgaggt ggcgcggaac cggcgggcgc gcagcgcgaa gctgcgctgt atcgagcggc
     5881 tggcgtcgta gggggcgtat gcgttacttt atctttgcgg cggtcgtggc ggcgtttctg
     5941 atcgtatacg tgtggcagaa cgtcgaggtg atgcgcatca agatggga
//
LOCUS       DAORHF010000227         5929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13575_length_5929_cov_5.950289, whole genome shotgun sequence.
ACCESSION   DAORHF010000227 DAORHF010000000
VERSION     DAORHF010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13575_length_5929_cov_5.950289"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PKJ16_15075"
     CDS             <1..901
                     /locus_tag="PKJ16_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HNV48364.1"
                     /translation="GAMGSAFAFLIGRTDRRVILLERDARVVSAVAGGLIVHDGECRE
                     TFRPEIGSDPAIIRDADPVFIFVKCHSTEAAMDDIAPAIGTATMVSLQNGIGNRELIA
                     RHVGEDAIVYGSVVLGATMIAPGEVSLRGGGSVVIGGRDARRVEDVRRLLARAGLAVS
                     VTDDPERAVWEKAIVNAAINPLGALLGVPNGVIAADPHAARIQEEVVREAVTVANGMG
                     IGADPDALIASVRDVCRITASNRCSMLQDLDAGRATEIDGINGAIVRIGKELGIATPW
                     NDELACLIRARERLGTPGGRCEP"
     gene            898..3102
                     /locus_tag="PKJ16_15080"
     CDS             898..3102
                     /locus_tag="PKJ16_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48365.1"
                     /translation="MSTTIREMGRIDELRHRMVDIGLLVSVIIGAPAALTSVLRAFET
                     GWRPVMIGHCVAFVLLAGSVAFKRWIGYHVRAFVLLAIGMTAGALGLFTYGLISMGIP
                     FLMATVVVSVILYGRWGGYVALGAGTLIIAAMGAGIVTGLIPLEFDIARYAVGPLSWV
                     TAGTAYACFMVIVVAMMGTYYGFLIDAVGALDARTGDLAAANERLEAEISERVKTEEA
                     LRATRAYLDNIINSMPSILVGISSDGSVLSMNRAAEAAAGRAERDVRGRPLVEVLPAF
                     AGHLDGAVATLRDGKTFDLQHAEVEWDGAMHTCSLTVFPLIMNGIRGGVVRIDDTTEV
                     VRREQRLRRLQKMDMIGTMAGGLAHDFNNILSGIMGSASILQHDLKDEDLRQRERIED
                     SIATIRHASRRAADTIRALMTISRKQENVFVPADLSRSIGNVVKLCESSFPPTVTIEH
                     RDAAPHERFIGVPARLEQVLLNVCMNAMHAMTIMRDAGEEQGGVVAIDSGVMTADDSF
                     AQAHPGAEAGSDYLYVRIADSGVGMDRMTRERAFEPFFSTKQDVSGTGLGLVMVDAIM
                     KQHRGIVDVESEPGVGTAVTLYLPRCHDEEQATGEASALEQRTSGAAGTVLVIDDDPI
                     VRGVTVKMLDSCGYAILTATGGEEGIMQYRDHLGRIDAVFLDMSMPGISGVDVFEELR
                     RIDPCARVLLSSGFTEDDRMERLVREGDAAFLPKPFTMTQLDAKLREVIGSA"
     gene            3099..3230
                     /locus_tag="PKJ16_15085"
     CDS             3099..3230
                     /locus_tag="PKJ16_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48366.1"
                     /translation="MMEHVVMGALRRPNARCGECAITVGLDEPFVPAAGCSSPACAA"
     gene            complement(3315..4280)
                     /locus_tag="PKJ16_15090"
     CDS             complement(3315..4280)
                     /locus_tag="PKJ16_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HNV48367.1"
                     /translation="MLDALLLIGGLAIIIAAANLLVEGASSIAHKLRVSDLAIGLTIV
                     AFGTSAPELAVSVTAAASGNTTLALGGVIGSNIANILLILGCSAIVYPLAIQESTQWK
                     EVPFSLMAVIAVAICANDLIIDGAGPNRISRIDGLILLLFFSIFLYYSYRLAKRGGAA
                     EPDAHVRELPVWRSIAYIVIGLGGLFLGGQMLVKGAVGVAMRIGMSESMVGLTIVAVG
                     TSLPELATSIVAAIKKKPDIAVGNVVGSNIFNIFFVLGATAVITPIPLAAASNIDIMV
                     AMTATVLLFMTAFTFRERTIDRTEGIVFVALYIGYIVYLVVTQIN"
     gene            4436..>5929
                     /gene="ilvD"
                     /locus_tag="PKJ16_15095"
     CDS             4436..>5929
                     /gene="ilvD"
                     /locus_tag="PKJ16_15095"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNV48368.1"
                     /translation="MKRELRSAMSTQGRRMAGARALWRANGMNEGQFGRPIIAIANSF
                     TQFVPGHVHLHEIGQHVKAVIESQGCFAAEFNTIAIDDGIAMGHAGMLYSLPSRDLIA
                     DSVEYMCNAHCADALVCITNCDKIIPGMLMAAMRINIPAIIVSGGPMEAGVWNGKKYD
                     LIDAMVMSADPAVSDDDVAAIERAACPTCGSCSGMFTANSMNCLCEALGLALPGNGTV
                     VATHANRIAIFEKAARRIVEMADAYYRNGDESVLPRSIATKAAFMNAMALDIAMGGST
                     NTVLHLLAVAHEAGVDFTMRDIDALSRKVPNLCKVAPSSHFHVEDVNRAGGILSIMGE
                     LERAGLIDGAARRVDFPTVGDALNAFDVMRATATDEARELYSSAPAGAGRNLVMGSQN
                     TMHPELDRDRTNGCIRDIAHAYSADGGLAVLFGNIATKGCIVKTAGVDPSIWKFSGRA
                     KVFFSQEAACDGILGGTIAAGDVVVIRYEGPKGGPGMQEMLYPTSYIK"
BASE COUNT         1030 a         1855 c         2036 g         1008 t
ORIGIN      
        1 gggcgccatg ggctcggcgt tcgccttcct gatcggccgt acggatcggc gcgtgatcct
       61 gctcgagcgt gacgcgcgcg tggtgtccgc cgtcgccggc ggcctgatcg tgcatgacgg
      121 cgagtgtcgc gaaaccttcc ggccggagat cgggtccgat cccgccatca tccgcgacgc
      181 cgaccccgtg ttcatcttcg tgaagtgcca ttccacggaa gcggcgatgg acgacatcgc
      241 gcccgcgatt gggacggcca cgatggtctc gttgcaaaac gggatcggca atcgggaact
      301 aatcgcacgg cacgtcggcg aagacgcgat cgtctatggg tccgtcgtgc ttggcgcgac
      361 catgatcgcg cccggcgagg tgtccctgcg cggcggcggt tccgtcgtca tcggcggccg
      421 cgatgcgcgt cgtgtggaag acgttcgccg gctgctggcg cgggccggcc tcgcggtctc
      481 cgtgacggac gatccggaac gtgcggtgtg ggagaaggcg atcgtgaacg ccgcgatcaa
      541 tcccctcggc gcgctcctgg gcgttcccaa cggcgtgatc gccgcggacc cgcacgcggc
      601 gcgcattcag gaggaggtcg tccgcgaggc cgtcacggtc gcgaacggca tgggaatcgg
      661 ggccgatccc gacgcgctga tcgcatcggt gcgcgatgtc tgtcgcataa cggcatccaa
      721 ccgctgctcg atgctgcagg acctcgacgc cggccgcgcg accgagatcg acggcatcaa
      781 cggcgccatc gtccggatag ggaaggagct ggggatcgcc actccctgga atgatgagct
      841 cgcgtgcctg atcagggccc gggagcgcct cgggacgccg ggggggcggt gcgaaccatg
      901 agcacgacaa tccgggagat gggccggatc gacgaactcc gccatcggat ggtcgacatc
      961 ggtttgcttg tcagcgtcat catcggggcc cctgccgcac tgacgagcgt tttgcgcgcc
     1021 ttcgagaccg ggtggcggcc tgtgatgatc gggcattgcg tcgccttcgt ccttctggcg
     1081 ggcagcgttg cgttcaagag atggattggg taccatgtcc gcgcgttcgt cctgctcgcc
     1141 atcggcatga ctgccggcgc cctggggttg ttcacctacg gcctcatcag catggggatc
     1201 ccgttcctca tggcgaccgt cgttgtctcc gtgatcctct atggccggtg gggcggctat
     1261 gtcgcgctcg gcgccggaac gctcatcatc gcggcgatgg gagcaggcat cgtcaccggc
     1321 ctcatccccc tcgagttcga catcgcgcgc tatgccgtgg gtcccctgtc ctgggtgacg
     1381 gcggggacgg cctatgcctg cttcatggtg attgtcgttg ccatgatggg cacatactat
     1441 gggtttctga tcgacgccgt cggggccctc gacgcacgca cgggcgatct ggccgccgcg
     1501 aacgaacggc tggaggctga gatctcggaa cgggtgaaaa ccgaggaggc gctgcgggcc
     1561 acccgcgcct atctcgacaa tatcatcaac tcgatgccgt cgatcctcgt ggggatctcg
     1621 tctgacggtt cggtcctgtc gatgaaccgg gcggcggagg cggccgcggg gcgtgccgaa
     1681 cgggatgttc gcggcaggcc gttggtcgag gtgcttcccg cgttcgccgg tcatctcgac
     1741 ggcgccgtgg cgaccctccg cgacggcaaa acgttcgacc tgcagcatgc cgaggtcgaa
     1801 tgggacgggg cgatgcatac ctgttcgctg accgtgttcc cgctgatcat gaacggaatc
     1861 cgcggcggtg tcgtccgcat cgacgatacg acggaggtgg tccgccgcga gcagcgactt
     1921 cgccgattgc agaagatgga catgatcgga accatggcgg gcgggctcgc ccatgacttc
     1981 aacaatatcc tgagcggcat catggggagc gcgagcatcc tgcagcacga cctgaaggac
     2041 gaggacctgc gccagcgcga acgcatcgag gactctattg ccaccatccg ccatgcctcg
     2101 cggcgcgcgg cggacaccat ccgcgcgctg atgaccatct cccggaagca ggagaacgtt
     2161 ttcgttcccg ccgatctttc gcggtctatc gggaatgtcg tgaagctctg cgaaagcagc
     2221 tttccgccga ccgtcacgat cgagcaccgc gatgccgcgc cgcacgagcg gttcatcggc
     2281 gtccccgctc ggttggagca ggtgctcctc aatgtctgca tgaacgcgat gcacgccatg
     2341 accatcatgc gggacgccgg cgaggaacag ggaggcgtcg ttgcgatcga cagcggcgtg
     2401 atgacggccg atgattcgtt cgcccaagcg caccccggcg cggaggcggg atccgactac
     2461 ctgtatgtga ggatcgccga tagcggggtc ggcatggacc ggatgacgcg cgaacgggcc
     2521 ttcgagccgt tcttctcgac caaacaggac gtgagcggca ccgggctcgg gctggtcatg
     2581 gtggacgcca tcatgaaaca gcatcggggc attgtcgatg tggagtcgga gccgggcgtc
     2641 gggaccgcag tcacgctgta tctgccccgc tgtcacgatg aggaacaagc aaccggcgag
     2701 gcgagcgcgc tcgagcaacg aacctccggc gcggcgggaa cggtgctggt gatcgacgat
     2761 gatcccatcg tgcgcggcgt tacggtcaag atgctcgact catgcggcta tgcgatcctg
     2821 accgcgacgg gcggcgagga ggggatcatg caatatcgcg accatctcgg ccggatcgac
     2881 gcggtttttc tcgacatgtc catgcccggg atctcgggcg tcgatgtgtt cgaggagttg
     2941 cgaagaatcg atccgtgcgc cagggtgttg ctgtcgtccg gtttcaccga ggacgatcgg
     3001 atggaacggc tggtgcgcga gggcgatgcg gcgtttctcc cgaagccgtt cacgatgact
     3061 caactcgatg ccaagctgcg ggaggtcatc ggaagcgcat gatggagcac gtcgtgatgg
     3121 gcgcgctgcg acggccgaac gctcgctgcg gtgaatgcgc catcacggtg gggctggacg
     3181 agccctttgt gcccgcggcc gggtgttcgt cgccggcatg cgcggcgtga ggcgaatcct
     3241 tcccgcgctc cccgaccgaa acgccgagat agccgggcgg cttcacggcc tccaacgcac
     3301 acgcgcgggc ggtcttagtt gatctgcgtt actacgaggt agacgatgta gccgatgtag
     3361 agcgcgacga agacgattcc ctcggtgcgg tcgatcgtcc gttcgcgaaa cgtgaaggcg
     3421 gtcatgaaca ggaggacggt ggccgtcatc gccaccatga tgtcgatatt gctcgcggcg
     3481 gcgagcggaa tgggggtgat gaccgcagtc gctccaagca cgaaaaagat gttgaagatg
     3541 ttcgatccga ccacgttccc caccgcgatg tccggtttct ttttgatcgc ggccacgatc
     3601 gacgtggcca gttccggcag cgaggtcccc acggccacga tggtgagccc aaccatcgat
     3661 tcggacatac cgatgcgcat cgcgacgccg accgcgccct tcacgagcat ctgcccgccg
     3721 aggaagagcc cgcccagtcc gatgacgatg tacgcaatcg aacgccagac gggcagctcg
     3781 cgcacgtgcg cgtccggttc ggcggcgcca ccgcgcttcg cgaggcggta gctgtaatag
     3841 aggaatatcg agaaaaagag gaggaggatg agcccgtcga tgcgcgagat gcggttgggc
     3901 cccgcgccgt cgatgatgag atcgttcgcg caaatggcca cggcgatgac cgccataagc
     3961 gagaagggca cctccttcca ctgggtgctc tcctggatcg ccaggggata gacgatcgcc
     4021 gagcagccga ggatgagcag gatgttcgcg atgttgctgc cgatcacccc tcccagcgcc
     4081 agcgtcgtgt ttcctgacgc ggccgcggtg acgctcaccg cgagctcggg cgcggaggtc
     4141 ccgaaggcga cgatggtgag tccgatcgcc aggtcggaga cgcgcagctt gtgcgcgatg
     4201 gacgaagcgc cctcgaccag gaggttcgcg gccgcgatga tgatcgccag tccgccgatg
     4261 agcaggagtg cgtcgagcat ggcgaatatc tgacggcggc aggtcgtgcg ctgtcaacca
     4321 gttttccgcg gcggcggcgc gcaataaagt tcttgcatca aacacggttt ctcgctatgt
     4381 gcggtagcga cgtgatggtg cggcatcatc aacattaata gcgcgaggtg cggacatgaa
     4441 gcgggaactg agaagcgcga tgagcacgca ggggcggcgc atggccgggg cgcgggcgct
     4501 ctggcgagcg aacgggatga acgaggggca gttcggcagg cccatcatcg ccatcgcgaa
     4561 ctcgttcacg cagttcgtgc cggggcacgt gcacctgcac gagatcggcc agcacgtgaa
     4621 ggccgtcatc gagtcgcagg gctgtttcgc cgcggagttc aacaccatcg ccatcgacga
     4681 cgggatcgcg atgggccacg ccgggatgct ctactcgctc ccgtcgcgcg acctcatcgc
     4741 cgacagcgtc gagtacatgt gcaatgcgca ctgcgccgac gcgctggtgt gcatcaccaa
     4801 ctgcgacaag atcatcccgg gcatgctcat ggccgcgatg cggatcaaca tccccgccat
     4861 tatcgtctcc ggcggtccca tggaggcggg cgtctggaac gggaaaaagt acgacctgat
     4921 cgacgcgatg gtgatgagcg cggacccggc cgtgtccgac gacgacgtcg ccgcgatcga
     4981 gcgcgcggcc tgtcccacct gcggatcgtg ttccgggatg ttcaccgcga attccatgaa
     5041 ctgcctctgc gaggcgctgg ggctcgcgct tccgggcaac ggcaccgtgg tggccaccca
     5101 cgcgaaccgg atcgcgatct tcgagaaggc cgcgcgccgg atcgtcgaga tggcggacgc
     5161 gtactaccgg aacggcgacg aatcggtgct gccgcgctcc atcgccacga aggccgcctt
     5221 catgaacgcc atggcgctcg acatcgccat gggcggatcg accaacaccg tgctgcacct
     5281 gctggcggtc gcgcacgagg ccggcgtcga tttcaccatg cgcgatatcg acgcgctctc
     5341 gcggaaggtg cccaatctgt gcaaggtcgc gcccagctcc cacttccacg tcgaggacgt
     5401 gaaccgcgcc ggcgggatcc tctccatcat gggcgagctc gagcgcgcgg gactcatcga
     5461 cggcgcggcg cggcgggtcg atttcccgac ggtcggcgac gcgctcaacg catttgatgt
     5521 catgcgcgcg acggcgaccg acgaagcgcg cgaactgtac tcaagcgcac cggccggcgc
     5581 ggggcggaac ctggtgatgg gatcgcaaaa cacgatgcac cccgagctcg atcgcgaccg
     5641 gacgaacggc tgcatacgcg acatcgccca tgcctacagc gccgacgggg ggctcgcggt
     5701 cctgttcggc aatatcgcca ccaagggctg catcgtaaag accgccggcg tggacccgtc
     5761 gatctggaaa ttctccggga gggcgaaggt cttcttctcg caggaggccg cctgcgacgg
     5821 catcctgggc ggtaccattg cggcgggtga cgtcgtggtg atccgctacg agggcccgaa
     5881 gggcggcccc ggcatgcagg agatgctcta tcccacctcg tatatcaag
//
LOCUS       DAORHF010000228         5926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13585_length_5926_cov_5.887413, whole genome shotgun sequence.
ACCESSION   DAORHF010000228 DAORHF010000000
VERSION     DAORHF010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13585_length_5926_cov_5.887413"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PKJ16_15100"
     CDS             <1..231
                     /locus_tag="PKJ16_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48369.1"
                     /translation="LTSREAAKLRAKQFGDQFIGLSLKEFASYDVAAGTGAGRIAAIA
                     NPAPGADTDFRASLRAVKKNLILMDEFVFSAR"
     gene            256..1455
                     /locus_tag="PKJ16_15105"
     CDS             256..1455
                     /locus_tag="PKJ16_15105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV48370.1"
                     /translation="MITIRTIAYKNLLRKKTRTILTVVGIALSAWVLSSLFGFNRGYE
                     SALNKDIDNMGFQIMVTAKGCPYEAATLMLKGGTGLRYMNEDIMKDVLKHQEVKQITP
                     MLMQAVFDPNKGESGGMSAYLGVDPTTIASMKGFLEFKQGAWFTNPEGLEVVMGYEAA
                     ELEQREAGDLLLVTPQPGKSVELKVVGILKRTGTQDDGTIFIPLKTLQRLFNNTGKIT
                     SIGVKVQKEADIAAFEEKLYQLPDVQVVSLVQVKRTIISLVATARVMVLSIAVIAILI
                     AMIGVINTILMSVYERYQEIGIIKSMGAMPGDVFRLVWIETMILCLVGGVIGNAAAVA
                     LAQATDVLIKRLLPYTPTGSIVQIDATLFAVTTAIIVGVGILSGLYPAWKASRVRPLE
                     AIRGEVS"
     gene            1452..2162
                     /locus_tag="PKJ16_15110"
     CDS             1452..2162
                     /locus_tag="PKJ16_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48371.1"
                     /translation="MTTNGIAIDAKNLVKIYDRGSERIAAVNDISLQIRTGEYVSFVG
                     PSGSGKTTLINLLGCLDNPSAGSLAIAGTEIFGEGRTLSERMLTTIRRERYGYIFQNF
                     YLIPTLTVLENVTLPFAFYRKENARDDIDRLLSHLGLEGRTGHLPGQLSGGEMQRVAI
                     ARALVNRPEILLADEPTGNLDPARGAEVGAVLKRLNEEEGITVITVTHNPALAATAGR
                     TIEIRDGRIAERAVTPPA"
     gene            complement(2146..3381)
                     /locus_tag="PKJ16_15115"
     CDS             complement(2146..3381)
                     /locus_tag="PKJ16_15115"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48372.1"
                     /translation="MKKKTREWFGNRHQLLSDYGTREDAYAFVLDRLIKRVEYNKTEA
                     GIRGLVKGRVKGFDAFYRKLLEKNVDRAIANPFEDIADIVGLRVVVPFLEDVHSIETL
                     IKKSFDVLEVENKSRELSIGEFGYDSTHVTVEVPQDILDEAGLAEILVCEIQIRTILQ
                     DAWAEVEHELVYKTGIDNSAVEASIRRKLIALNATLSLADITFQELRDYQKKRAQDVQ
                     VRHQKLLDKVSTIPEKMALRAGGDAANMARDETAAGSETSESNTMFMEALDAHLDNRL
                     ERALELYTQILLETPNHYIYNHRGLVYFAMSDYRKAVDDFTAAIELQPGDTRVYTNRG
                     LAYRMLREYESALADFERSIDINPFWADTFYGRALTHYDMGNVSAALADCDVAIDIKP
                     GFKQVVRFKQFLLSIMQGA"
     gene            3640..4449
                     /gene="larE"
                     /locus_tag="PKJ16_15120"
     CDS             3640..4449
                     /gene="larE"
                     /locus_tag="PKJ16_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HNV48373.1"
                     /translation="MDIEHKLESLKAALAGMESVLVAFSGGADSTLLLRIAIDVLGDR
                     VLAVTARSETYPEEELAAAAAIATTLGARHRFIDTNELENETYAENPPDRCYHCKREL
                     YGALGALAAAEGLRHVIDGNNLDDETDYRPGAKAAAELGVVSPLREAGLRKEEIRSLS
                     RAMGLPTWDKPAMPCLASRFPYGTRITPQRLAMVAMAERHLRGLGIRQLRVRFHGDLA
                     RIEVSPGDMPRLIDPDTAAAITKRFREIGFTFVALDLQGYRMGSLNEALEG"
     gene            4560..>5926
                     /locus_tag="PKJ16_15125"
     CDS             4560..>5926
                     /locus_tag="PKJ16_15125"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV48374.1"
                     /translation="MPDTARSESGARARKIRFATATALFDGHDASINIIRRILQQEGA
                     EVIHLGHNRSVKEIVFTAIQEGVQAIAVSSYQGGHIEFFKYMKDLLDENGAGFIKIFG
                     GGGGVIVPEEIEELESYGIEKIYSPEDGQHLGLVGMIRDMIARAGSVTHPPLPDDWRE
                     KIREHNSFEITHLISIAEKDYADKKSIYKAIGKEIRELGGSSPVIGMTGTGGSGKSSL
                     EDEFIRRFLHHYPDKRIAVLAIDPTRKKTGGALLGDRIRMNFLDPERVFMRSLATRES
                     KTELSSAITDSIAVLRAAGYDLILIETPGIGQGDTTVTEFCDFAVYVMTSEYGAPTQL
                     EKIDMLDYADLVVLNKFDHKNSMDALRDIRKQYRRNHNIFGGPDEELPVVGTVASNFL
                     DPGLNVFFAKLLGLLKAKGLIDWEVPGVETYSPGDTSKSAVIPLDRENYLWEISNTVA
                     SYHRKS"
BASE COUNT         1153 a         1916 c         1856 g         1001 t
ORIGIN      
        1 cttacctcgc gcgaggcggc gaagctgcgc gcaaagcaat tcggggacca gttcatcggc
       61 ctgtcgctca aggaattcgc gtcatacgat gtcgccgcgg gcacgggcgc cggacgcatt
      121 gcggccatcg ccaatccagc gccgggcgcg gacacggact ttcgcgcctc gctgcgcgcg
      181 gtgaagaaaa acctcatcct catggatgag ttcgtattca gcgcccgctg acgtcgggcg
      241 caaggaggaa cgccaatgat cacgatacgc accatcgcat acaaaaatct tctgcggaag
      301 aagacgcgca ccatcctcac cgtcgtgggg atcgcgctct ccgcgtgggt gctctcgagc
      361 ctcttcggct tcaaccgggg ctatgagagc gcgctcaaca aggacatcga caacatgggc
      421 ttccagatca tggtgaccgc gaaggggtgc ccctacgagg cggccacgct catgttgaag
      481 ggggggacgg gcctgcgcta catgaacgag gacatcatga aggacgtcct gaaacaccag
      541 gaggtgaagc agataacgcc catgctcatg caggccgtgt tcgaccccaa caagggtgag
      601 agcggcggga tgagcgccta cctcggcgtc gatccgacca ccatcgcctc catgaagggc
      661 ttcctcgagt tcaaacaggg cgcgtggttc acaaatccgg aagggctcga ggtggtgatg
      721 ggttacgagg cggccgagct cgagcagcgc gaggcgggcg acctcctgct cgtcacgccg
      781 caaccgggga agagcgtcga gctcaaggtc gtcggcatcc ttaagcggac gggcacgcag
      841 gacgacggca ccatcttcat accgctcaag acgctgcagc ggctcttcaa caacaccggt
      901 aagatcacca gcatcggggt caaggtgcag aaggaggcgg acatcgccgc gttcgaggag
      961 aaactctatc agcttcccga cgtgcaggtc gtctcgctcg tgcaggtcaa gcggaccatc
     1021 atcagcctgg tcgcgaccgc gcgggtgatg gtgctctcga tcgcggtgat cgccatcctc
     1081 atcgcgatga tcggcgtcat caacaccatt ctcatgtcgg tgtacgagcg ctaccaggag
     1141 atcggcatca tcaagagcat gggggcgatg cccggcgacg tgttccgcct ggtctggatc
     1201 gagacgatga tcctgtgcct ggtcggcggc gttattggaa acgccgcggc cgtcgcgctc
     1261 gcccaggcga ccgacgtgct catcaagcgc ctgcttccct acacgccgac cggaagcatc
     1321 gtgcagatcg acgcgacgct ctttgcggtc acgaccgcga tcatcgtcgg ggtcggcatc
     1381 ctgagcggtc tctacccggc atggaaggcg tcgcgggtca ggccgctcga agcgatacgg
     1441 ggggaggtgt catgaccacg aatgggatcg caatcgacgc taagaatttg gtgaaaatct
     1501 acgatcgcgg cagcgaacgg atcgccgccg tgaacgacat atcgctccag atacggaccg
     1561 gggaatacgt ctctttcgtc gggccgtccg gttccggcaa gactaccctg atcaacctgc
     1621 tgggatgcct cgacaacccc agcgccggaa gtctcgcgat agcggggacg gagatattcg
     1681 gggaaggccg cacgctctcg gaacgcatgc tcacgacgat ccggcgcgag cgctacggct
     1741 acatcttcca gaatttctat ctcatcccca cgctcacggt gctcgagaac gtgacgctgc
     1801 ccttcgcgtt ctaccgcaag gagaacgcgc gggacgacat cgaccgcctg ctgtcgcacc
     1861 tcgggctcga ggggcgcacg gggcacctgc cgggacagct ctcgggcggg gagatgcagc
     1921 gcgtcgccat cgcgcgggcg ctcgtgaacc ggccggagat attgctcgcc gacgagccca
     1981 ccgggaacct cgatcccgca cgcggcgcgg aggtgggggc ggtgttgaag cgcctcaacg
     2041 aggaggaggg gatcaccgtg atcacggtca cgcacaatcc ggcgctcgcg gcgacggcgg
     2101 gacggacgat cgagatcagg gacgggagga tcgccgagcg cgcggtcacg ccccctgcat
     2161 gatggaaagc aggaactgct tgaagcggac cacctgcttg aagcccggct tgatgtcgat
     2221 ggcgacgtcg cagtccgcga gcgcggcgct cacgttcccc atgtcgtagt gcgtgagcgc
     2281 gcgcccgtag aaggtgtcgg cccagaaggg gttgatgtcg atggagcgct cgaaatcggc
     2341 cagggccgac tcgtactcgc gcagcatgcg ataggcgagc ccgcggttgg tgtagacgcg
     2401 cgtgtcgccg ggctgcagct cgatcgccgc ggtgaaatcg tccaccgcct tccggtaatc
     2461 cgacatggcg aagtacacca gcccgcgatg attgtagatg taatgattgg gcgtctccag
     2521 caggatctgc gtatagagct caagcgcccg ctccagccgg ttgtccaggt gcgcgtcgag
     2581 cgcctccatg aacatcgtgt tcgactcgga cgtttcgctc ccggcggccg tctcgtcgcg
     2641 cgccatgttc gcggcgtcgc ccccggcgcg cagcgccatc ttctcgggga tcgtggacac
     2701 cttgtccagg agcttctggt ggcgcacctg gacgtcctgc gcccgctttt tctgataatc
     2761 gcgcaactcc tgaaaggtga tgtccgcgag cgacagggtg gcgttgagcg cgatcagctt
     2821 gcggcggatc gacgcctcga ccgcgctgtt gtcgatcccc gtcttgtaga cgagctcgtg
     2881 ctccacctcg gcccatgcgt cctgcaggat ggtgcggatc tggatctcgc acacgaggat
     2941 ctcggccagc cccgcctcgt cgagaatgtc ctgcggcacc tcgacggtga catgggtgga
     3001 atcatagccg aactcgccga tggagagctc ccgcgacttg ttctccacct cgagcacgtc
     3061 gaaggatttc ttgatgagcg tttcgatgga atggacgtcc tcgaggaagg ggaccaccac
     3121 gcgcaacccg acgatatcgg cgatatcctc gaagggattg gcgatggcgc gatcgacgtt
     3181 cttctcgagg agcttgcggt aaaaggcgtc gaagcccttt acgcgcccct tgaccagccc
     3241 ccggataccc gcttcggttt tattgtattc cacgcgcttg atgagccggt cgagcacgaa
     3301 cgcgtacgcg tcctcccgcg tcccataatc ggacaagagc tggtgacgat tgccaaacca
     3361 ctccctcgtt tttttcttca ttgcgcgcct tcccggtgca gtgccccgcc cgacggacgg
     3421 gtgttgtcaa atgaggcttc gcgatcggtg gattgttaca gacaatagcg taccgaacat
     3481 cacggaaaaa gtcaagcgga tattccctcg agaacgacgg gatcgcgcgc tcgcgcatca
     3541 tgcgctcgtc ccagtggact gtttttttcg tcgttgataa tttcatcgcc gatgataccg
     3601 ttgccggaac actcatcgca tggcatgcac ggagctcaca tggacatcga acacaagctt
     3661 gaatcgctga aggccgcgct tgcgggcatg gaaagcgtcc tggtcgcctt ctccggcggc
     3721 gccgacagca ccctcctgct gcggatcgcg atcgacgtat tgggcgatcg cgttctcgcc
     3781 gtcaccgcgc gctcggaaac ctaccccgag gaggagctcg ccgccgccgc cgcgatcgca
     3841 accacgctgg gcgcgcgcca ccgcttcatc gacaccaacg agttggaaaa cgaaacctac
     3901 gcggaaaacc ctcccgatcg ctgctaccac tgcaagcgcg agctctatgg cgcgctcggc
     3961 gcgctcgccg cggcggaggg cctgcgccac gtgatcgacg gcaacaacct cgacgacgaa
     4021 accgactacc gcccgggcgc gaaggccgcc gcggagctgg gggtcgtaag cccgctcagg
     4081 gaggccgggc tccgcaagga ggagatacga tcgctgtcgc gcgcgatggg gctgcccacg
     4141 tgggacaagc cggccatgcc gtgcctcgcc tcgcgctttc cctacggcac ccgcatcacc
     4201 ccccaacgcc tcgcaatggt cgccatggcc gagcggcatc tgcggggcct ggggatccgc
     4261 cagctgcgcg tgcgcttcca cggcgacctc gcgcgcatcg aggtgtcgcc cggggacatg
     4321 ccgcgcctga tcgatcccga caccgcggcg gcgataacca aacgctttcg ggagatcggc
     4381 ttcaccttcg tcgcgctcga cctgcaggga taccgcatgg ggagcctcaa cgaggctctg
     4441 gaagggtgag cgcctgaatt ctcattgcca aaaaaatagt ttgacacgac cctgccaccg
     4501 ctgatactgg ctaaaaccga gcgaacgctc ggtcgattta gtgacccacg gaggacccca
     4561 tgcccgacac cgcacgatcc gaatccggcg cccgcgcgcg caagatacgc ttcgcgaccg
     4621 ccaccgcgct ctttgacggg cacgacgcct cgatcaacat catccgccgc atcctgcagc
     4681 aggagggcgc cgaggtgatc cacctggggc acaaccgctc cgtgaaggag atcgtgttca
     4741 ccgccatcca ggagggggtg caggccatcg cggtgagttc ctatcagggc ggacacatcg
     4801 agttcttcaa atacatgaag gacctgctcg atgagaacgg cgcggggttc atcaagatct
     4861 tcggcggcgg gggcggcgtg atcgtccccg aggagatcga agagctcgag tcctacggca
     4921 tcgaaaagat ctattcgccc gaggacggcc agcacctggg gctggtgggc atgatccgcg
     4981 acatgatcgc gcgcgccggg agcgtcacgc acccgcccct ccccgacgac tggcgcgaaa
     5041 agatccggga gcacaacagc ttcgagatca cccacctcat ctccatcgcc gagaaggact
     5101 acgccgacaa gaagagcatc tacaaggcca tcgggaagga gatccgcgag ctcggcggat
     5161 catcgcccgt gatcggcatg acggggaccg gcggctcggg caagagctcg ctcgaggacg
     5221 agttcatcag gcgcttcctg catcactacc cggacaagcg catcgcggtc ctcgccatcg
     5281 accccacgcg gaagaagacc ggcggggcgc tcctgggcga ccgcatccgc atgaactttc
     5341 tggacccgga gcgcgtcttc atgcgctcgc tcgccacgcg cgaatcgaaa accgagctct
     5401 ccagcgccat caccgattct atcgccgtgc tacgcgcggc cggctacgac cttatcctca
     5461 tcgagacgcc ggggatcggc cagggcgaca cgacggtgac cgagttctgc gacttcgccg
     5521 tctacgtgat gacctcggaa tacggcgcgc ccacccagct cgagaagatc gacatgctgg
     5581 actacgccga cctcgtggtg ctcaacaagt tcgaccacaa gaactcgatg gacgcgttgc
     5641 gcgacatacg aaagcagtac cgtcgcaatc acaacatctt cggcgggccc gacgaggagc
     5701 tgccggtcgt ggggaccgtc gccagcaact tcctcgaccc ggggctcaac gtcttcttcg
     5761 cgaagctcct gggcctcctc aaggccaagg gcctcatcga ctgggaggtg cccggcgtcg
     5821 agacctactc gcccggcgac acgtcgaaat ccgcggtgat cccgctggat cgcgagaact
     5881 acctctggga gatatcgaac acggtcgcct cgtatcaccg aaaatc
//
LOCUS       DAORHF010000229         5924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13593_length_5924_cov_5.952632, whole genome shotgun sequence.
ACCESSION   DAORHF010000229 DAORHF010000000
VERSION     DAORHF010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13593_length_5924_cov_5.952632"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="PKJ16_15130"
     CDS             complement(<1..695)
                     /locus_tag="PKJ16_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV48375.1"
                     /translation="MIPRTGQRTHRARAALLAAGAAFSLLIASAPGAAGELSFGVNAG
                     ATYDVNRLGEDINRYNVVMEAYSDENPGSMTQSMGTPVAPAFGLSARYQFNQLLFRVG
                     LYFATPVGSIEGYITPADGQENRLKISTFQISMPATVALVLPIKEKTYVYLGLGLTYH
                     QIYVKMSQSNPQQTAALFEANGLSSHRTDQYGGQFGGYHILIGAEFPLLERLTLTVDW
                     IHQEARSQALDET"
     gene            complement(692..1483)
                     /locus_tag="PKJ16_15135"
     CDS             complement(692..1483)
                     /locus_tag="PKJ16_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48376.1"
                     /translation="MRTYIILLVVLIAFAAAPRPLAAAITTNGIGIYTGIAPNMGGSM
                     HSFVQQQHFNTRNGIHDINRVQDGIDTAKIERPMGVTVGIDWKVIIEEHFFFRAAANY
                     SKSVVGGSGTTAWTDNGGISYYALDCRYSVATFDVPLLAGFTIKFWDDVRFYVGGGAA
                     FASGMYDNEFSSEDHPDPFTRKGQFRGTGFPLVAYAHGEYYFTTRYAISATVLYYGGS
                     TPLLRDGIHADTEGLNGKGAYDYARIDFTGYRFAFGITFYFGSDQ"
     gene            complement(1498..2157)
                     /locus_tag="PKJ16_15140"
     CDS             complement(1498..2157)
                     /locus_tag="PKJ16_15140"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HNV48377.1"
                     /translation="MKRTIARTACLLAAAAIVATARPARAAETFAAGVHGGFQHDVGN
                     FAAYNPAVEVDPENTFLLGASFFLNYGRGFFRFGFDTTILFNRGRIIEDPGQVERINR
                     YAINYTALPAFIGIRFDIFDRGAVYLGAGATYFLASGRINFKSTDAEEEITANIVGYG
                     FILGGQINLTRNACLYLEWQYVSGRSQALLNTSAEYTWYNLALDMTGNRYFLGCRYYL
                     F"
     gene            2366..5317
                     /locus_tag="PKJ16_15145"
     CDS             2366..5317
                     /locus_tag="PKJ16_15145"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48378.1"
                     /translation="MGQKRIRNSLAIALIPFFLMAMMSAAAADTTLCEVVITHPPDLR
                     PYAFVDERGESKGVAIDLMRIWGRKVECGVRFVPAEAAKGVELLKAGKADIVGGMHYY
                     GEFHDEIDFAREFLEIEVDLFADKAVPIKSIDGVGPHPVGVVAGGFPEKWLQAQYPQV
                     ALRRFAGNEDLVRSALAGGVAAFVLEYPVAGYYLARHGAHDRFTRIETLYLEKLRVGV
                     RRGNAELLKRINAGMHRITFREVREVFRQWSIMPAIPEWLKRYLWLAGAGAVFVLLLV
                     FIVVLRVKVRHRTRRIEEVLDEARVQNRRLETEIQERQRAEDALADERNLLRTIINNI
                     PDIVFALDGEGKALVLNDAGRRYLAPKDREPIAGRDIAEFLPEDVVLKCRQAGSLIDL
                     DPNLALSEEGRFTGPDGGELWFLDTAVPLRDEDGAVMGMVCISRDITRQKLAEISMRR
                     ALNELESFVTTIPDMFYVLDTAGRLIKWNRKFEEATGYDSAELVSRNIAHLFTPMCGS
                     EATCLVEEHRRLGRGNSLEVYVRNRSGELILYEFVTSALKDENSAVIGYVGIGRDITE
                     RHRYEDALRVSEERFRSIFDLADVSIWEIDYEAIRGAVEEILASGVTDIRAYLDEHPD
                     DFDRIKRGVRIVDVNEASVKLYGASSKEELIAGIEKIFLAESIEAFKDVIAAVASGSR
                     FYQVETRRRTMQGAIITVILRVSVPAPESAFRNILITTIDVTEQKRHEAALQAAKDAA
                     EIANRAKSTFLANMSHELRTPLNSVIAMSDMLVESYLGELNPEQADYVRDIRESGRHL
                     LNLINDILDLSKVEAGYSPLEPGPVDLAQLVANSLVVVRERALKHGIALSIDVAGTLP
                     EIVADERKVKQIVFNLLSNAVKFTGDGGSVGVRAAARDGGVELCVWDTGIGIAPENID
                     RVFGEFYQVEETLTKRYEGTGLGLSLVKRFAEQHGGHVRVESEPGKGSRFYVFLPAVL
                     ADTAVRGGDGA"
     gene            5314..5694
                     /locus_tag="PKJ16_15150"
     CDS             5314..5694
                     /locus_tag="PKJ16_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48379.1"
                     /translation="MSAPTVLIVEDNPKNRKIMADLLRHNGYRFIEAEDGQAGVEKAI
                     AERPDLILMDIQLPRMDGYEATRRLRANEATRGIPIIVITSFAMKDEEARARAAGCDE
                     YMTKPIDIHAFVATVKRYAPPRGE"
     gene            5703..>5924
                     /locus_tag="PKJ16_15155"
     CDS             5703..>5924
                     /locus_tag="PKJ16_15155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48380.1"
                     /translation="MQPRTEHVGPVLIVEDNPQNQKILKGLCARQGLATAVADNGRVA
                     LDMLSDGFFAVYIVDLMMPVMDGRAFIGHL"
BASE COUNT         1067 a         1745 c         2075 g         1037 t
ORIGIN      
        1 aaagtctcgt cgagcgcctg cgagcgcgcc tcctggtgta tccagtccac ggtgagcgtg
       61 agccgctcga ggagggggaa ttcggccccg atcaggatgt ggtagccgcc gaactgcccg
      121 ccgtactggt ccgtgcggtg cgacgagagc ccgttggcct cgaacagggc cgccgtctgc
      181 tgcgggttcg attgggacat cttgacgtag atttgatgat aggtgagccc gagacccagg
      241 tatacatagg tcttttcctt gatgggcagc acgagggcga cggtcgcggg catcgagatc
      301 tggaaggtgg agatcttgag ccggttctcc tggccgtccg cgggggtgat gtacccctcg
      361 atggagccga cgggcgtggc gaagtacagg cccacccgga agaggagctg gttgaactgg
      421 tagcgcgccg agagcccgaa ggcgggcgcg acgggcgttc ccatcgactg cgtcatggag
      481 ccggggtttt cgtcgctgta ggcctccatg accacgttgt agcggttgat gtcctcgccc
      541 aggcgattca cgtcgtaggt cgcgccggca ttcacgccga acgagagctc gcccgccgct
      601 ccgggcgccg atgcgatgag cagggaaaac gccgcgcccg ccgcgaggag cgcggcccgc
      661 gcccgatgcg tccgttgtcc ggtgcgtggt atcattgatc ggacccgaag tagaacgtga
      721 tgccgaaggc gaaccggtac ccggtgaagt cgatgcgggc gtagtcgtag gcgcccttgc
      781 cgtttaagcc ctcggtgtcg gcgtggatgc cgtcgcgcaa cagcggcgtg gacccgccat
      841 agtagagcac cgtcgccgag atcgcgtatc gtgtggtgaa gtaatactcg ccgtgcgcat
      901 aggcgaccag cggaaatccg gtcccccgaa actgcccctt gcgcgtgaag ggatcggggt
      961 ggtcctccga cgagaactcg ttgtcgtaca tgccggaggc gaaggcagcg ccgccgccca
     1021 cgtagaagcg cacgtcgtcc cagaacttga tggtgaagcc cgcgaggagc ggcacgtcga
     1081 aggtggccac ggaatagcgg caatcgagcg catagtagga gatcccgccg ttgtcggtcc
     1141 aggcggtcgt gccgctcccg cccacgacgc tcttggaata attcgcggcg gcccggaaga
     1201 agaaatgttc ctcgatgatg accttccagt cgatgcccac cgtgacgccc atcgggcgtt
     1261 cgatcttcgc cgtgtcgatg ccgtcctgca cgcggttgat gtcgtggatg ccgtttcgcg
     1321 tgttgaagtg ctgctgttgc acgaatgagt gcatcgaccc gcccatgttc ggcgcgatgc
     1381 cggtatagat gccgatgccg ttggtggtga tcgccgccgc aagcggccgg ggggccgcgg
     1441 cgaacgcgat cagcacgacg agcaggatga tgtatgtgcg caccgtattc attgccgcta
     1501 aaagagatag tacctgcatc caagaaaata ccggtttccg gtcatgtcca gcgcgagatt
     1561 ataccaggtg tactccgcgc tcgtgttcag cagggcctgc gaccgcccgc tcacatactg
     1621 ccactccagg tacaggcagg cgtttcgcgt gaggttgatc tgtccgccca ggatgaagcc
     1681 gtagcccacg atgttcgccg tgatctcctc ctcggcgtcg gtcgatttga aattgatcct
     1741 gccggaggcg agaaagtagg tggccccggc gccgagatac accgcgccgc ggtcgaagat
     1801 gtcgaaccgg atcccgatga aggccgggag cgcggtgtag ttgatggcgt agcggttgat
     1861 gcgctcgacc tggcccgggt cctcgataat acgcccgcgg ttgaacagga tggtcgtgtc
     1921 gaagccgaag cggaagaaac cccgcccgta attcaggaag aaggaggcgc ccagcagaaa
     1981 ggtgttctcg ggatcgacct cgacggcggg attgtaggcg gcgaagtttc ccacgtcgtg
     2041 ctggaagccg ccgtgcacgc cggcggcgaa ggtctccgcg gcgcgcgccg gacgggcggt
     2101 cgcgacgatg gccgcggcgg cgaggaggca agctgttcgt gcgatcgttc gtttcatcgt
     2161 ttgcgtatgg gcgcgttcgt ggtcggcgcc ggtccggatg cggcgtgcgc cccgtggcgg
     2221 ctcctgatta ctatgatcgg ccggtttcgg gcgggaattg aggcaattta accgccgggg
     2281 tgatcacggt gcgaaaatgg atggacagca tggcttccgc atggtagcgt gaaatacggc
     2341 atccagaatg ctttggaaac atgccatggg ccagaaacgc atccgaaaca gtctcgccat
     2401 cgcgctcatc cctttctttt taatggcgat gatgagtgcc gccgcggccg atacgacgct
     2461 gtgcgaagtc gtcatcaccc acccgcccga cctgcgccca tacgccttcg tggacgagcg
     2521 cggcgagtcg aagggcgtcg ccatcgacct catgcggatc tgggggcgga aggtcgagtg
     2581 cggggtgcgc ttcgttcccg cagaggccgc gaagggcgtg gagctgttaa aggcggggaa
     2641 ggccgatatc gtcggcggca tgcactatta cggcgagttt catgacgaga tcgatttcgc
     2701 ccgcgagttt ctcgagatcg aggtggacct gttcgcggac aaggcggtgc cgatcaaatc
     2761 gatcgacggc gtggggcccc atcccgtggg cgtcgtcgcc ggcggttttc cggagaagtg
     2821 gctgcaggcg cagtacccgc aggtcgcgct gcgccggttc gcgggcaatg aggacctcgt
     2881 tcgcagcgcg cttgccggcg gtgtcgcggc cttcgtgctc gagtatccgg tggccgggta
     2941 ctaccttgcg cgtcacggcg cgcacgaccg cttcacacgg atcgaaacgc tgtatctgga
     3001 aaagctgcgc gtgggggtgc gtcgtgggaa cgcggagctg ttaaagcgca tcaatgccgg
     3061 gatgcaccgc atcaccttcc gcgaggtgcg ggaggtcttc cggcagtggt cgatcatgcc
     3121 ggcgattccg gaatggctca agcgctacct gtggctggcc ggggccggcg cggtgttcgt
     3181 cctgctgctc gtgttcatcg tcgtgctgcg cgtcaaggtg cggcaccgga cgcggcgcat
     3241 cgaggaggtg ctggacgagg cgcgcgtgca gaaccggcgg ctggagacgg agatccagga
     3301 gcgccagcgc gccgaggacg ccctggccga cgagcgcaat ctcctgcgga ccatcatcaa
     3361 caatattccc gatatcgtgt ttgcgctcga tggcgaggga aaagcgttgg ttctcaacga
     3421 cgcgggtcgc cgctacctcg cgccgaagga ccgggagccg atcgccggtc gggacatcgc
     3481 ggagtttttg ccggaggatg tcgttctcaa gtgccgccag gccgggtcgc ttatcgatct
     3541 cgatccgaac ctcgccttgt ccgaggaggg gcgcttcacc ggcccggacg gcggcgagct
     3601 gtggttcctc gacaccgcgg tgccgctgcg cgacgaagac ggcgccgtga tgggcatggt
     3661 gtgcatctcg cgcgacatca cccgccagaa gctcgcggag atatcgatgc gccgcgcgct
     3721 caatgaattg gaaagcttcg tgacgacgat ccccgacatg ttttacgtgc tggacacggc
     3781 ggggcggctc atcaagtgga accggaaatt cgaggaggcg accgggtacg attccgcgga
     3841 gctggtgagc cggaacatcg cgcacctctt cacgccgatg tgcggatcgg aggccacctg
     3901 tctggttgag gagcaccgcc gcctcgggcg ggggaactct ctggaggtgt atgtgcgcaa
     3961 ccggagcggg gagctgattc tctacgagtt cgtcacctcg gcgctcaagg acgagaacag
     4021 cgcggtgatc gggtacgtgg gcatcggtcg cgacatcacc gagcgccacc ggtacgagga
     4081 cgccctgcga gtttcggagg agcgcttccg gagcatcttc gatctggccg acgtctcgat
     4141 ctgggagatc gattacgagg cgatccgggg ggcggtcgag gaaatcctgg ccagcggcgt
     4201 gaccgatatc agggcctacc tcgacgagca tccggacgat ttcgaccgga tcaagcgggg
     4261 cgttcgcatc gtcgacgtga acgaggcgtc ggtgaagctc tacggcgcct cgtcgaagga
     4321 ggagttgatc gcgggcatcg agaagatttt tctcgccgag tccatcgagg ccttcaagga
     4381 cgtcatcgcc gcggtcgcca gcgggagccg cttctatcag gtggaaacgc gccgccgcac
     4441 gatgcagggc gcgatcatca ccgttatcct gcgcgtgagc gtccccgcgc cggagagcgc
     4501 cttccggaac atcctcatca ccaccatcga tgtgaccgaa cagaagcgcc acgaggcggc
     4561 cctgcaggcg gcgaaggatg ccgcggagat cgccaaccgc gcgaaatcga ccttcctggc
     4621 gaacatgagc cacgagctgc gcaccccgct caattccgtg atcgcgatgt ccgacatgct
     4681 cgtcgagagc tacctgggcg agctgaatcc cgagcaggcg gactatgtgc gcgacatccg
     4741 ggagagcgga cggcacctgc tgaacctgat caacgacatc ctggaccttt cgaaggtgga
     4801 ggccgggtac agcccgctcg agcccggccc ggtggacctg gcgcagcttg tggccaacag
     4861 cctggtggtg gtgcgcgagc gggccctgaa gcacgggatc gcgctctcga tagacgtggc
     4921 ggggacgttg cccgaaattg tcgcggacga acggaaggtg aagcagatcg tctttaacct
     4981 gttgtcaaac gccgtgaagt tcaccgggga cgggggatcg gtgggcgtcc gcgccgcggc
     5041 gcgcgacggc ggcgtggagc tgtgcgtgtg ggacaccggg atcggcatcg ccccggagaa
     5101 catcgaccga gttttcggcg agttctatca ggtcgaggag accctcacga agcgatacga
     5161 ggggacggga ctgggactgt cgctggtgaa gcgcttcgcc gaacagcacg gcggccatgt
     5221 gcgcgtggag agcgaaccgg ggaagggcag ccgcttttac gtgttcctcc ccgcggtttt
     5281 ggcggatacg gcggttcggg gaggtgatgg cgcatgagcg ctccaacggt cctgatcgtg
     5341 gaggacaatc caaaaaaccg gaagatcatg gcggacctgc tgcgccacaa cggctatcgc
     5401 ttcatcgagg cggaggacgg gcaggcgggc gtggagaagg ccatcgccga gcggccggac
     5461 ctcattctca tggacatcca gctcccgcgc atggacggct acgaggcgac gaggcgcttg
     5521 cgggcgaacg aggccacgcg ggggattccg atcatcgtga tcacctcgtt cgcgatgaag
     5581 gacgaggagg cgcgcgcccg cgcggcggga tgcgacgagt acatgaccaa gcccatcgac
     5641 atccacgcgt tcgtcgcgac cgtgaaacgg tatgcgccgc cgagggggga gtgatacgcc
     5701 atatgcagcc gagaacggaa catgtcggac cggtcctgat cgtcgaggac aatccgcaga
     5761 accagaagat cctgaagggg ctctgcgcgc gccaggggct cgcgaccgcg gtcgccgaca
     5821 acgggcgcgt cgcgttggac atgctctcgg acgggttctt cgccgtctac atcgtcgatc
     5881 tcatgatgcc ggtgatggac ggaagggcct tcatcggcca tctc
//
LOCUS       DAORHF010000230         5909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13644_length_5909_cov_4.998463, whole genome shotgun sequence.
ACCESSION   DAORHF010000230 DAORHF010000000
VERSION     DAORHF010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13644_length_5909_cov_4.998463"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="PKJ16_15160"
     CDS             <1..504
                     /locus_tag="PKJ16_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48381.1"
                     /translation="YLQRDYGLISNMSAEQNIRLPLQYHSTLSSAETRAVADRLIDAL
                     NLEGCRARRPINLTMSETLRTAFARSLILEPDLLLVEHALEGQCLINSQTFLRELERW
                     ADRPGCSLMMTTYEPDRFLVCADRFMMLYDGRIVFEGTREEYLHTDNPFVRQYKLASV
                     DGPMRIL"
     gene            516..1253
                     /locus_tag="PKJ16_15165"
     CDS             516..1253
                     /locus_tag="PKJ16_15165"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HNV48382.1"
                     /translation="MLKLDSNEIRVAAFIVVPLALLLLIVAVKLGYSIEGSTIDVYLK
                     VDSISSIKKGTNVKIKGHTIGRVVEIQPVYKPALHFLATMRIDNTVGLFENCAAVIQN
                     QNIIGDPVIELRNPERPADLLAGGSVIEGIEHVNLEVILQDVHNLLANTNAAIGVFKE
                     IQLQSKGNIRSMMQNLANSVANLNSILANSQRDIVAILSSFRETAKTFTEISAELKKR
                     PMGFIIRGKKDEPGEKKPDEQKPEAKK"
     gene            1262..2125
                     /locus_tag="PKJ16_15170"
     CDS             1262..2125
                     /locus_tag="PKJ16_15170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV48383.1"
                     /translation="MGGPFFAVVIPVHNRPGELARAVDSVLAQTCRDFELVVVDDGST
                     DATSEVAASYGDRIRLIRQENAGVSAARNRGIAATRAPWIALLDSDDAWRPDKLARQR
                     RFAEERPDCSILQANEIWVRNGRRVNPPETHRKRDGRIFRESLARCMVSPSAVALRRD
                     LFERYGPFDERLPACEDYDLWLRITAFEPVGLIDEDLLTRYGGHDDQLSRRFWGMDRF
                     RVHAIVRLLEEYGERLGPGDREAAIAHRSSAAGAARRGNAAFAARMDALGGEIRSGRY
                     STVACGFLVEA"
     gene            complement(2073..3149)
                     /locus_tag="PKJ16_15175"
     CDS             complement(2073..3149)
                     /locus_tag="PKJ16_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48384.1"
                     /translation="MKFLIIGLVAGIALGFAPWWPHRTGTELYPEWHGSLAYLNEIEP
                     TAHPALAARALPPIALGNTLVVLAATGAERSRFDLGESHAAASRDGRFLVRYEKVGKS
                     VEYLNMAGEPFWKLRSLEYPHISPNGKLVLLLNGDQTRVRFVDNNGNEIGAKEASGRL
                     CTQIAFAKRTDRACVAFLDGSWLVVDARGEIRASGVLPGNPPIKSAAVSDGGAFAALH
                     YGGAKHDCVAVVRTDDKKIKVIELSSAHLTRTGLFVSDDGSVAVIDREAVLVTEGFSR
                     RGESIRIPARRPGHARIDRSAGLYVASYTRETGEAAWLLFRPDGTVLMHRELPEESFL
                     DCHIEDGLMLLRGSRTLQCYSVRS"
     gene            complement(3153..4205)
                     /locus_tag="PKJ16_15180"
     CDS             complement(3153..4205)
                     /locus_tag="PKJ16_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013254306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HNV48385.1"
                     /translation="MDKKPSRKKPRASRTPDARRDDDAPLEEPLDDEERDEAVGDDEE
                     SARGDERDDDAEELDEAPVRGSKKASRSKSGPPSSISWYLDQINKIPLLTREEEDVLA
                     RAARDGDPKAKDRLIRANLRFVVSIAKKYQTSGISLLDLINEGNMGLIKAADKFDPDR
                     GFHFISYAVWWIRQAIILAISQKTSLIRLPLNRTADMQKIERVHKALENKFGREPTPG
                     EIAEELDMDDDEINHLRAISQDYISLDASYGDSEDTSILSMVEDSRIDAPDKGVLDES
                     LTSALNAVLGTLTSSEREILKMRFGLDGYKPMSLQQIGEKFKLSKERIRQIEKKAIRR
                     LRHPSRSQKLKSFLQD"
     gene            4534..5601
                     /gene="rfbB"
                     /locus_tag="PKJ16_15185"
     CDS             4534..5601
                     /gene="rfbB"
                     /locus_tag="PKJ16_15185"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013968784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HNV48386.1"
                     /translation="MRTLKNILVTGGAGFIGSNFIRYLFERVGFPGAIVNVDKLTYAG
                     NPESLDDIRQSYGGQYHFEHADIGDFERMRDILARYDIDTVVNFAAESHVDRSIFGPK
                     DFVQSNIVGTFTLLEAVRAHWGKRRDTLFHHVSTDEVYGSLGETGAFYETTPYDPRSP
                     YSASKAASDHLVMAYHHTYELAVTISNCSNNYGPYQFPEKLIPLMIMNLREGKPLPVY
                     GDGRQVRDWLHVDDHNSAVWLIMNRGANGETYNIGGENERENIWIVHTLCEAMARITG
                     NSVDSYKRLITHVADRPGHDRRYAINCDKIKGALGWTRAVAFDAGLESTIRWYLANDA
                     WVNRVRSGEYRNWIERNYGKR"
     gene            5588..>5909
                     /locus_tag="PKJ16_15190"
     CDS             5588..>5909
                     /locus_tag="PKJ16_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HNV48387.1"
                     /translation="MGSDKAARTLIATAAFGLESVVASELHRLGYGDLATENGKVTFP
                     ATDADIARCNINLRCADRVLLQMGQFRATDFEELFQGVKAIPWEGLIPENGVMHVTGK
                     SVRSK"
BASE COUNT         1023 a         1883 c         1971 g         1032 t
ORIGIN      
        1 tacctgcagc gggactacgg gctcatcagc aacatgagcg cggagcagaa catcaggctg
       61 cccctgcagt accactcgac gctctcctcg gccgaaacgc gcgcggtggc ggaccggctc
      121 atcgacgcgc tcaatcttga gggatgccgc gcgcggcggc ccatcaacct tacgatgtcg
      181 gagacgctgc ggacggcgtt cgctcgatcg ctcatcctcg agcccgacct gctcctggtc
      241 gagcacgccc tcgaggggca atgccttatc aactcgcaga ccttcctgcg cgagctcgag
      301 cgctgggccg accggcccgg ctgctccctg atgatgacca cctacgagcc ggatcgcttt
      361 ctcgtgtgcg ccgatcgctt catgatgctc tacgacggtc gtatcgtgtt cgaggggacg
      421 cgtgaggagt atctgcacac cgacaatccc ttcgtgcggc agtataaact ggcgtcggtc
      481 gacgggccga tgcgcattct gtgaggaggc ggacgatgct gaagctggat tcgaacgaga
      541 tcagggtggc ggcgttcatc gtcgtgccgc tcgcgctgct gttgctcatc gtcgcggtga
      601 agctgggcta ctccatcgag ggctccacca tcgacgtcta tctcaaggtc gacagcatct
      661 cgtcgatcaa gaagggcacg aacgtgaaga tcaaggggca cacgatcggc cgcgtggtgg
      721 agatacaacc cgtctacaag ccggcgctgc acttcctggc cacgatgcgc atcgacaaca
      781 ccgtgggcct cttcgagaac tgcgcggcgg tgatccagaa ccagaacatc atcggcgatc
      841 cggtcatcga gctccgaaac cccgagcgcc cggccgacct cctggccggc ggttcggtga
      901 tcgagggtat cgagcacgtg aaccttgagg tgattctgca ggacgtgcac aacctgctgg
      961 cgaacaccaa cgcggccatc ggcgtcttca aggagattca gctgcagagc aaggggaaca
     1021 tccgcagcat gatgcaaaac ctcgccaaca gcgtcgcgaa cctcaacagc atcctggcga
     1081 actcgcagcg cgacatcgtg gccatcctct cgtcgttccg cgagaccgcg aagacgttta
     1141 ccgagatatc ggccgagctg aaaaaacggc ccatgggctt catcatccgc gggaaaaagg
     1201 acgagccggg cgagaagaaa cccgacgagc agaagccgga ggcgaagaag tagatccggc
     1261 cgtgggcggc cctttcttcg cggtggtgat ccccgtccat aaccggccgg gggagcttgc
     1321 gcgggcggtc gactcggtgc tcgcgcaaac gtgccgcgac ttcgagctcg tcgtggtcga
     1381 cgacggctcg accgacgcca ccagcgaggt cgccgcatcc tacggggacc gcatcaggct
     1441 gatccgccag gagaacgcag gggtgagcgc cgcgcgcaat cgcggtatcg ccgccacgcg
     1501 cgccccctgg atcgcgctcc tggactcgga cgacgcatgg cgtccggaca agctcgcgcg
     1561 gcagcgccgc ttcgcggagg agcgtccgga ctgctccatc cttcaggcga acgagatctg
     1621 ggtgcgaaac ggccggcgcg tgaatccgcc ggagacgcac cgcaagcgcg acgggcggat
     1681 cttccgcgag tcgcttgcgc gctgcatggt gagcccgtcg gcggtggcgc tgcgccggga
     1741 cctcttcgag cgctatgggc ccttcgacga gcggctcccc gcctgcgagg actacgacct
     1801 ctggttgcgc ataacggcat tcgagccggt gggcctcatc gacgaggacc tgctcacgcg
     1861 gtacggcggg cacgacgacc agctctcgcg ccgcttctgg gggatggacc ggttccgcgt
     1921 ccacgcgatc gtgcggctgc tggaggaata cggggagcgc ctcggcccgg gcgaccggga
     1981 ggcggccatc gcgcaccgct ccagcgcggc gggcgccgcg cgccgcggga acgccgcgtt
     2041 cgccgcgcgg atggatgcgc tcggggggga gatcaggagc ggacgctata gcactgtagc
     2101 gtgcggcttc ctcgtagaag catgagcccg tcctcgatgt ggcagtccag gaacgactcc
     2161 tcggggagct cgcggtgcat gagcaccgtc ccgtcgggcc ggaagaggag ccaggcggcc
     2221 tcgccggtct cgcgggtgta gctcgccacg tagaggcccg cggagcggtc gatgcgcgca
     2281 tgtccaggtc ggcgcgcggg gatgcggatc gactcgccgc ggcgcgagaa gccctcggtc
     2341 accagcacgg cctcgcggtc gatcacggcg accgagccgt catcggacac gaagagcccc
     2401 gtccgggtga ggtgcgcgct cgaaagctcg atcaccttga tttttttgtc gtcggtgcgc
     2461 accacggcga cgcagtcgtg cttcgccccg ccgtaatgca gcgcggcgaa cgcgccgccg
     2521 tcgctcaccg ccgcgctttt gatgggcggg ttgccgggaa ggacgcccga ggcgcggatc
     2581 tccccgcgcg cgtccacgac gagccagctc ccgtcgagaa aggcgacgca ggcgcggtcc
     2641 gtccgcttgg cgaaggcgat ctgcgtgcac agccggccgc tcgcttcctt cgcgccgatc
     2701 tcgttgccgt tgttgtccac gaagcgcacg cgggtctggt cgccgttgag gagcagcacc
     2761 agcttgccgt tgggcgaaat gtgcggatac tccagcgagc gcagcttcca gaacggctcc
     2821 ccggccatgt tcaggtactc cacggacttg cccaccttct cgtagcgcac caggaagcgg
     2881 ccgtcgcggc tggccgccgc gtgcgattcg cccaggtcga agcgcgagcg ctcggcgccg
     2941 gtggcggcga ggacgaccag cgtgtttccc agcgcgatcg gcgggagcgc gcgcgcggcg
     3001 agcgccgggt gcgcggtcgg ctcgatctcg ttcaggtagg ccagggagcc gtgccattcc
     3061 ggatagagct ccgttccggt ccgatggggc caccacgggg cgaacccgag cgcgatgccg
     3121 gcgacaagtc cgatgatgag gaatttcatt gatcaatcct gcaggaagct tttcaatttt
     3181 tggctccgcg aggggtgccg gagccggcgg atggccttct tttcgatttg tcggatgcgc
     3241 tccttggaga gcttgaactt ctcgccgatc tgctgcagcg acatgggctt gtagccgtcc
     3301 aggccaaagc gcatcttgag gatctcgcgc tccgacgagg tgagggtgcc cagcaccgcg
     3361 ttcagggcgc tggtgagcga ctcgtccagc acgcccttgt cgggcgcgtc gatgcggctg
     3421 tcctccacca tggagaggat ggaggtgtcc tcggaatcgc cgtagctggc gtccagcgag
     3481 atgtagtcct gcgagatggc gcgcaggtgg ttgatctcgt cgtcgtccat gtccagctcc
     3541 tcggcgatct cgccgggggt gggctcgcgc ccgaacttgt tctcgagggc cttgtggacc
     3601 cgctcgatct tctgcatgtc ggctgtgcgg ttgagcggca gccggatgag cgaggtcttc
     3661 tgcgagatcg ccaggatgat cgcctggcgg atccaccaca cggcgtagga tatgaagtgg
     3721 aagccgcggt ccggatcgaa cttgtccgcc gccttgatga gccccatgtt gccctcgttg
     3781 atgaggtcca gcagggagat gccgctggtc tggtacttct tcgcgatgga caccacgaag
     3841 cgcaggttcg cgcggatgag gcggtccttc gccttcgggt cgccgtcgcg cgcggcgcgg
     3901 gcgagcacgt cctcctcctc gcgcgttaag agcgggatct tgttgatctg gtccaggtac
     3961 caggagatgg acgagggcgg gcccgatttc gagcgcgacg ccttcttcga gccgcgaacg
     4021 ggcgcctcgt cgagttcctc ggcgtcatcg tcccgctcgt ctccgcgggc gctctcctcg
     4081 tcgtcaccga cggcttcgtc gcgctcctcg tcgtcgaggg gctcctccag cggcgcatcg
     4141 tcgtcgcgtc gcgcgtctgg ggtgcgggac gcccgcggtt ttttgcgtga tggtttcttg
     4201 tccatgtcgt ggagcattct tgcggacatt gcattgaaaa tcaagtgtat tttcctgaac
     4261 agatatcgcc atcagtatat tcatttttta cagtgctgat tgcgggggca ttcgcgcccg
     4321 ctaagcggta cgaaacatcg aggacgacat cggcttgctt ctgggtgtcg aaatccttgc
     4381 ggccgtgctg atataatgaa aaaccctcag ggagtcgaca ccgggcgtcg ggggcttatt
     4441 ggcgcgatga ttcctgcgga gcgaaaaagt gttgtaaaaa ccatcgcgcg ggtgcacgtg
     4501 acctatcacc aggccgcatg tacgggagga gtgatgcgca cgcttaaaaa cattctcgtg
     4561 accggcgggg ccgggttcat cggatcaaac tttatccgct atctcttcga gcgcgtcggt
     4621 ttccccggcg cgatcgtcaa cgtggacaag ctcacctatg cgggcaaccc cgagagcctg
     4681 gacgacatcc ggcaatcata cggcgggcag tatcatttcg agcacgccga catcggtgat
     4741 ttcgagcgca tgcgcgacat cctcgcgcgc tacgacatcg acaccgtcgt gaacttcgcg
     4801 gccgagtcgc acgtggaccg ctccatcttc gggcccaagg acttcgtgca gtccaatatc
     4861 gtgggcacct tcacgctgct cgaggccgtg cgcgcgcact ggggcaagcg acgcgacacg
     4921 ctcttccacc acgtgagcac cgacgaggtg tatggatcgc tgggcgagac cggcgccttt
     4981 tacgaaacga cgccgtatga tccgcgcagt ccctactcgg cgtccaaggc cgcgtcggac
     5041 cacctggtga tggcgtatca tcacacctac gagctcgcgg tgacgatctc gaactgcagc
     5101 aacaactacg ggccctacca gttccccgag aagctcatcc cgctcatgat catgaacctg
     5161 cgcgagggga agccccttcc cgtatacggt gacggccggc aggtgcgcga ctggctgcac
     5221 gtggacgacc acaacagcgc cgtgtggctc atcatgaacc gaggcgcgaa cggcgagacc
     5281 tacaatatcg gcggcgagaa cgagcgcgag aacatctgga tcgtgcacac gctctgcgag
     5341 gcgatggcgc gcataacggg gaattccgtc gattcgtaca agcggctcat cacccatgtg
     5401 gccgaccggc cgggacacga ccgccgttac gccatcaact gcgacaagat caaaggggcg
     5461 ctgggctgga cgcgggccgt ggcgttcgat gcggggctgg agtcgaccat ccgctggtat
     5521 ctggcgaacg acgcgtgggt gaaccgcgtg cgctcgggcg agtaccggaa ctggatcgag
     5581 cggaactatg ggaagcgata aggcggcccg caccctcatc gccaccgcgg ccttcggcct
     5641 cgagtcggtg gtcgcgagcg agctgcatcg actgggctac ggggaccttg cgaccgagaa
     5701 cggcaaggtg accttcccgg cgaccgacgc cgacatcgcc cgctgcaaca tcaacctgcg
     5761 ctgcgccgac cgcgtgctcc tgcagatggg ccagtttcgc gcaacggact tcgaggagct
     5821 cttccagggc gtgaaggcca tcccctggga ggggctcatt cccgagaacg gcgtgatgca
     5881 cgtgacgggg aagtccgtgc gctcgaagc
//
LOCUS       DAORHF010000231         5846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13856_length_5846_cov_4.915213, whole genome shotgun sequence.
ACCESSION   DAORHF010000231 DAORHF010000000
VERSION     DAORHF010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13856_length_5846_cov_4.915213"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PKJ16_15195"
     CDS             <1..641
                     /locus_tag="PKJ16_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HNV48388.1"
                     /translation="FTVGRGNVHAFPEAAYAARDAGADMIWADRAIPLGNAAGQSGES
                     ITSDEYAAFLEDMFAIRRRFSRALFSRTRVFMHRAVQFQPLLRHGEKNVAPYRCSAGR
                     NLLALMPDGALLPCRRMPIVVGTLPAEGLDEIYYRSALFAKLRRSAVTALGCAGCQHE
                     TSCNGGLRCLSYALTGDPFRRDPHCSLPHPQRQCVDDVPAPTTLSHPMPSIP"
     gene            683..2161
                     /locus_tag="PKJ16_15200"
     CDS             683..2161
                     /locus_tag="PKJ16_15200"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48389.1"
                     /translation="MITRTAFAVIIALILFGFAARPLSASYYSKGCGYYSAKKYDKAR
                     EMLQKAVEESPDNGNAYFFLGEVEKLTGNYHRARECYQTALGKKNLNPKYRKLAHWNL
                     SVLSDIKTDYPAMVRNLKAAWQKTGDAGSKKKVENLINKLMWSENAEAIELYKKGQQA
                     AQTGVATQAETHFREAMQKDPAFLAPRFEIGMIQYRDGRADAAAANFREIAAKIPFYG
                     EVHLLLGEIAYNARTYPAAVEHYGRALEFAFLDAAAEYEVVMKRATAALAADKPAEAK
                     RDLLRIGRKKITVEAALLLSSIYIRESAYADALKTLKRTGAAGRTNPEVVFQYGIIAH
                     LQKDEASIGHFDRLFDMTAGGAAPLPQKYYKAFRSNLKEHHANENHRRVIAIFEGLPE
                     AARDRESYAYAALAYYRTGDYARAIPCFTKHPAEGDALFMLARSYALTGARPAAKETL
                     ARMHGSPALFRKALADDALNGLAREVDAEKKSAPRPDGSPAK"
     gene            2142..3035
                     /locus_tag="PKJ16_15205"
     CDS             2142..3035
                     /locus_tag="PKJ16_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48390.1"
                     /translation="MALRPSNIVIVRLAALVLAVAVVSIANVRLGTLDVIGLLVFASG
                     ATVYLTLIKLGSADRQGRIDYLRSGEAAVATLVVFILTLLVTENVFALLGAVMLAFVA
                     YNVARRVLVGTHRRAQAIAGVITLVVCAAVLAHLFSSISVEPARLAGALPALGLPHAF
                     SGALYLLPLFMALSGAALYLFSRSLDEVAAFTQGARYFELTGGPFLPVALLLIVIRSA
                     FTAIVFLCLGWTAGTGAYLQRRMNAGRLTDDVTVGLAVLLYAHLIALTMPLAGPIGTI
                     AIATLLSHVAYAITAGRGNMS"
     gene            3032..3805
                     /locus_tag="PKJ16_15210"
     CDS             3032..3805
                     /locus_tag="PKJ16_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48391.1"
                     /translation="MIELRGIERHRGETTVLSIPQARFNDGEIIGLIGPPGAGKSCLL
                     SILGGVDEDYLGEALFDDTPLRSIKRRERRNLVALLDRTGPENHDETLYNFLLLSRIA
                     HKRFMEPFSDYDIQVVEEHLAHFELTAHRATPLCELTDSAYQRALVAFSLARTAPVTL
                     LDNPTSALDPRAQMLLHRALARAVMSGGAIVLIASNDLNFVLQTADRVIVLREGTVRA
                     DLGASAVEADLIREHFNAEVFVTRNIYNGRPSVHFFPES"
     gene            complement(3812..4159)
                     /locus_tag="PKJ16_15215"
     CDS             complement(3812..4159)
                     /locus_tag="PKJ16_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48392.1"
                     /translation="MKDIDYTVEDYRDIRVVTLSGNISNATKSELMALVRRIAQSASV
                     VINMTNVPMVTSSGLDALVEMCNAARSMDRRVLLMNVHDDIVKMIETLDLYDYFIFVE
                     SIEEGRRKLSYYV"
     gene            complement(4195..5193)
                     /locus_tag="PKJ16_15220"
     CDS             complement(4195..5193)
                     /locus_tag="PKJ16_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HNV48393.1"
                     /translation="MKRLKMGVIGVGHMGQYHVNVVNMLKDKFVLVGVYDANPDRAKE
                     IATKFETTAFGDVEDLVKKIECCIIAVPTSLHYQCATLALDNNVHVLVEKPVTDTAEQ
                     ARELAEKAKSKKLIFQVGHVERFNGAVQELSKTVNTPLIIESRRLSPFSPRIKDVGVV
                     LDLMIHDVDIVMNLVNSPVVRVSASGGSIYTAVEDYAHAVLQFESGCIASITSSRVTQ
                     RKIRTLSISQEKSYLFLDFSTQDIHIHRQASNAYLLTKENIKYSQESFVEELTVRKDN
                     PLKLEIEHFYDCVKNGATPMVSNTSDITILETTLEIERQVRSMLGRVQPSSVAAAK"
     gene            complement(5330..>5846)
                     /locus_tag="PKJ16_15225"
     CDS             complement(5330..>5846)
                     /locus_tag="PKJ16_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HNV48394.1"
                     /translation="LIGEPSLCTMLRENDLASIRRPDTLEELVFRSAGFKAGVVSQDE
                     REGGLRAILNFGHTAGHAIESCLEYRGIMHGAAVAAGMLIAIDLSRARGLLDERVARE
                     YRELAARYDLIPRGLALNAAQVVAHMRYDKKNAGGEIRFVLLAGVGEPRINQPVPEGE
                     LRAAVDDFLAG"
BASE COUNT         1024 a         2030 c         1720 g         1072 t
ORIGIN      
        1 ccttcaccgt cggaagagga aatgtgcacg ccttccctga ggccgcgtac gccgcgcgcg
       61 acgccggggc cgacatgatc tgggccgacc gcgcgatccc gcttggaaac gccgcagggc
      121 aatcgggcga atcgattacg agcgacgaat acgccgcatt tctagaagac atgttcgcaa
      181 tacggcgtcg tttttcgcgc gcgctcttct cgcgcacccg ggtgttcatg caccgggccg
      241 tgcagttcca gccgcttctg cgccacggcg agaagaacgt tgcgccctat cgctgctccg
      301 ccggcagaaa ccttctggcg ctcatgccgg acggcgcgct gcttccctgc cggcgcatgc
      361 ccatcgtcgt gggaacgctc cccgccgaag gcctcgacga gatatactat cgctcggcct
      421 tgttcgcgaa gctccgccgc tccgcggtca cggcgctcgg gtgcgcgggc tgccagcacg
      481 agacctcctg caatggcggc cttcggtgcc tctcctacgc cctcaccggc gatccctttc
      541 ggcgcgatcc ccattgctct ctgccgcacc cgcaacggca gtgtgtcgat gatgttcccg
      601 cccccacgac gctgtcgcac ccgatgccat ccatccccta atgtcgcttg acaactcagc
      661 gcgtttggcg gtacatcacc ccatgatcac acggaccgcg ttcgccgtca tcatcgcgct
      721 catcctgttc gggtttgccg cccgccccct ctccgccagc tactacagca agggctgcgg
      781 atactactcc gcgaaaaagt acgacaaggc gcgggagatg ctccagaagg ccgtcgagga
      841 atcgcccgac aatggcaacg catacttttt cctcggcgaa gtggagaagc tcacggggaa
      901 ctatcaccgc gcccgtgagt gctaccagac cgcgctcggc aaaaaaaacc tcaaccccaa
      961 ataccgaaag ctcgcccact ggaacctgtc ggtgcttagc gacatcaaga ccgactaccc
     1021 cgccatggtg cggaacctca aggcggcgtg gcagaagacg ggcgatgccg ggtcgaagaa
     1081 gaaggtcgag aacctcatca acaagctcat gtggagcgag aacgcggagg cgatcgagct
     1141 ctacaagaag ggccagcagg ccgcccagac gggcgtcgcg acgcaggcgg aaacgcactt
     1201 ccgcgaggcc atgcaaaaag atccggcctt cctcgcgccg cgcttcgaga tcggcatgat
     1261 ccaataccgc gacgggcgcg cggacgccgc tgccgcgaac ttccgcgaga tcgcggcgaa
     1321 gatccccttc tacggcgagg tgcatctcct gcttggcgag atcgcgtata acgcgcgaac
     1381 ctatccggcc gccgtcgagc actacggccg cgcgctcgag ttcgccttcc tcgacgcggc
     1441 ggcggaatac gaggtcgtca tgaagcgcgc gacggcggcg ctggccgcgg acaagccggc
     1501 cgaggcgaag cgcgacctcc tgcggatcgg ccgaaagaaa ataaccgtcg aggccgccct
     1561 gctcctgtcc agtatctaca taagagaatc cgcatacgcc gacgccctga aaaccctcaa
     1621 gcgtacgggg gcggcgggcc gaaccaaccc cgaggtcgta ttccagtacg gcatcatcgc
     1681 ccatctccaa aaggacgagg cctccatcgg gcacttcgac cgcttgttcg atatgaccgc
     1741 cggcggcgcg gcgccgctgc cccagaagta ctacaaggcg ttccggagca acctcaagga
     1801 gcaccacgcg aacgaaaacc accgccgggt aatcgcgata ttcgaggggc tgccggaggc
     1861 cgcgcgcgac cgcgagtcct acgcatacgc cgcgctggcc tactaccgca cgggcgatta
     1921 cgcgcgcgcg attccctgct tcacgaaaca ccccgccgag ggcgatgcgc ttttcatgct
     1981 cgcgcgctca tacgccctca ccggcgcgcg gcccgcggcg aaggaaacgc tcgcccgcat
     2041 gcacggctcc cccgcgctct tccgcaaggc ccttgcggac gatgccctga acggcctcgc
     2101 gcgcgaggtg gatgcggaaa aaaaatcagc gccgcgcccc gatggctctc cggccaagta
     2161 atatcgtcat cgtccggctt gcggcgctcg tcctggccgt cgccgtcgtt tcaattgcga
     2221 atgtccgcct ggggaccctc gacgtcatcg gcctcctcgt cttcgcgtcc ggcgcgaccg
     2281 tatacctgac gctcatcaag ctgggatcgg cggaccggca gggccggatt gattatctcc
     2341 ggtcggggga ggcggcggtt gcgacgctgg tcgtcttcat ccttacgctc ctcgtcaccg
     2401 aaaacgtttt cgcgcttctg ggcgcggtca tgctcgcatt tgtcgcgtac aacgttgccc
     2461 gccgcgtcct cgtcggcacg caccgccgcg cgcaggccat cgcgggcgtc atcacgctgg
     2521 tcgtctgcgc cgccgtgctc gcgcacctgt tctcttcgat atcggtcgaa cccgcacggc
     2581 tcgccggcgc cctccccgca ctcggcctgc cgcacgcgtt ttcgggcgcg ctctatcttc
     2641 tccctctttt catggcgctc tccggcgcgg cgctctacct tttctcgcgc agtttggatg
     2701 aggtcgcggc ctttacgcag ggcgcgcgtt acttcgagct caccggcgga ccgtttctgc
     2761 cggtcgcgct tctcctgatc gtcatccgaa gcgcctttac cgcgatcgtg ttcctctgcc
     2821 tgggatggac cgccggaacg ggcgcctacc tgcagcgccg catgaacgcc ggccgcctca
     2881 cggacgacgt caccgtcggg ctggccgtac tgctctatgc gcatctgatc gccctgacga
     2941 tgccgctggc gggcccgatc ggcaccatcg ccatcgctac gctcctgtcg catgtcgcct
     3001 acgctatcac cgccggaagg gggaacatgt catgattgaa cttcgcggaa tcgagcgcca
     3061 ccggggcgaa acgaccgtcc tgtccatacc gcaggcgcgg ttcaatgacg gggagatcat
     3121 cggtctcatc ggcccgccgg gcgcggggaa atcgtgtctg ctttcgatcc tgggcggcgt
     3181 cgacgaggac tatctcggcg aggcgctctt cgacgacacc ccgctgcggt ccatcaagcg
     3241 ccgcgagcga cggaatctcg tcgcgctact tgatcggacc ggccccgaaa accacgacga
     3301 gaccctgtac aactttctgc ttctttcgcg gatcgcccac aagcgcttca tggagccgtt
     3361 ttccgattac gacatccagg tggtcgagga gcacctggcg cacttcgagc tcaccgcgca
     3421 ccgcgccacg ccgctgtgcg agctcaccga cagcgcctac cagcgcgcgc tcgtggcctt
     3481 ctcgctcgcg cgcaccgcgc cggtgaccct ccttgacaat ccaacgagcg ctctcgatcc
     3541 ccgcgcgcag atgctgctcc atcgcgcact ggcgcgcgcc gtcatgagcg gcggcgcgat
     3601 cgtcctgatc gcgtccaatg atctcaactt cgtgctgcaa acggccgacc gggtgatcgt
     3661 cctgcgggag gggaccgtcc gcgccgacct gggcgcgtcg gccgtcgaag cggaccttat
     3721 ccgggagcac ttcaatgcgg aggtgttcgt tacgcgcaac atctacaacg gcaggcccag
     3781 cgtgcacttc tttccggaga gctaacggaa gctagacgta atagctcagc ttgcgccggc
     3841 cctcctcgat gctctccacg aagatgaaat aatcgtagag gtcaagcgtc tcgatcatct
     3901 tgacgatgtc gtcgtgcacg ttcattagga ggacgcgccg gtccatcgat cgcgccgcgt
     3961 tgcacatctc caccagcgcg tccagccccg acgaggtgac catcgggacg ttggtcatgt
     4021 tgatgacgac gctcgcactc tgcgcgatgc gccggaccag cgccatgagc tcgctcttgg
     4081 tggcgttgga tatgttgccg gagagcgtca cgacccggat gtccctgtag tcctcgacgg
     4141 tatagtcaat gtccttcacg gatcaccccc ggccgcgccg cgcacgtccc cgcgctactt
     4201 cgccgcggcg accgacgacg gctgcaccct ccccagcatg ctgcgcacct gccgctcgat
     4261 ctccagcgtg gtctcgagga tggtaatgtc ggaggtgttt gagaccatgg gcgtcgcgcc
     4321 gtttttcacg cagtcgtaga agtgctcgat ctccagcttc aagggattgt ccttgcgcac
     4381 cgtgagctcc tcgacgaacg actcctggct gtacttgatg ttctctttcg tgagcagata
     4441 ggcattgctc gcctgacggt ggatatggat gtcctgcgtc gagaagtcca ggaaaagata
     4501 ggacttctcc tggctgatcg acagggtgcg gatcttccgc tgcgttacgc gcgacgaggt
     4561 gatggaggcg atgcagccgc tctcgaactg cagcacggcg tgcgcgtagt cctcgaccgc
     4621 ggtgtagatg gagccgcccg aggcgctcac gcggacgacc ggcgaattga ccaggttcat
     4681 cacgatgtcc acgtcgtgga tcatgaggtc caggaccacg cccacgtcct tgatccgcgg
     4741 cgagaagggg ctcaggcgcc tcgactcgat gatgagcggc gtattgacgg tcttcgagag
     4801 ctcctgcacg gccccgttga accgctcgac gtgccccacc tgaaagatga gcttcttgct
     4861 cttcgccttc tccgcgagct cacgggcctg ctccgccgtg tcggtcaccg gcttttcgac
     4921 gagcacatgg acattgttat ccagcgcgag cgtcgcgcac tgataatgga ggctggtcgg
     4981 caccgcgatg atgcagcatt cgattttctt caccaggtcc tcgacgtcgc cgaacgcggt
     5041 cgtttcgaat ttcgtcgcga tctctttcgc ccgatccggg ttcgcgtcgt acacgccaac
     5101 caggacgaac ttgtccttga gcatgttcac cacgttcacg tgatactgcc ccatgtgacc
     5161 cacgccaatt acgcccatct tcaaacgttt catagtgcat tccttttgcg taccgattgt
     5221 tgttacgccc ctgcatccat ccgcgcccgc ggcatacgaa taggcatcta tccggccgaa
     5281 ccggaaaacg tcaattcgaa attgcggaca cggttcccga catgctccat caccccgcga
     5341 ggaagtcgtc aacggccgcg cgcaactccc cctcggggac cggctgattg atccgcggct
     5401 cgcccacgcc ggcaagcagc acgaaccgta tctccccgcc cgcgttcttt ttgtcgtagc
     5461 gcatgtgcgc aaccacctgc gccgcgttga gcgcgagccc gcgcgggatg agatcgtaac
     5521 gcgccgcgag ctcacgatac tcgcgtgcga cccgctcgtc taatagtccc cgcgcgcgcg
     5581 acaggtcgat cgcgatcagc atccccgccg cgaccgccgc gccgtgcatg atcccgcggt
     5641 actcgaggca cgattcgatg gcgtgacccg ccgtatggcc gaagttgaga atggcccgaa
     5701 ggccgccctc gcgctcatcc tgcgacacaa cacccgcctt gaaacccgcc gagcgaaaca
     5761 ccagctcctc gagcgtgtcc ggacgtcgaa tcgaggcgag atcgttttcc ctgagcatcg
     5821 tacacagcga cggctcgccg atgagg
//
LOCUS       DAORHF010000232         5835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13906_length_5835_cov_3.374740, whole genome shotgun sequence.
ACCESSION   DAORHF010000232 DAORHF010000000
VERSION     DAORHF010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13906_length_5835_cov_3.374740"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PKJ16_15230"
     CDS             <1..664
                     /locus_tag="PKJ16_15230"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48395.1"
                     /translation="DFYILREENRSRIAFYKNSIIHYFIPLSYCALALLADARRGGVT
                     TTTTLAQEYASIRELMRREFVHSSAEGADAPEREERAALQYLIGAGMVVADGETVRIG
                     DQAADTLKFYAKILREYLESYYIVLGAVLDYRRGKVNRKDFTVQVRRKGIQLYHIGIV
                     GLAEALSLPNYNNAISVCIDNRILSEEVTGKKTSTITLLDPGRAQGMMDRIKTYLEVV
                     VG"
     gene            complement(828..1433)
                     /locus_tag="PKJ16_15235"
     CDS             complement(828..1433)
                     /locus_tag="PKJ16_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48396.1"
                     /translation="MNIKQFVIIAVICALCALVPVSGYALFDIGAYGGGNYTEYKPGV
                     TKFDSYGGQYGFVAHLNTTGMDLWVIDLEIGIGGFFDRGNFYYTRNYNGYQVNKTSYG
                     PDIRVLVDIPFIIDPYVRGGAAAGEQLDMTDFSIRGNDGSRGEMGHAWGAYYYGGGID
                     ITFFPFLRFFAEYIRYTSTYKEPRNLTFIQNQLNLGLRLNL"
     gene            complement(1574..3367)
                     /locus_tag="PKJ16_15240"
     CDS             complement(1574..3367)
                     /locus_tag="PKJ16_15240"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HNV48397.1"
                     /translation="MKSTDIPALNDIIHEISEIEVELESFIDTTMWISDPHGAGERFV
                     SILKGRFGLVWQTCREALPKTFSNDKIDYLGRLIRKERYFATESYTMERQDVISALVK
                     ITQYKVQNSINFNQIRDTINNNLRPFLENLILNMPVPNIMYENDLIAERVISSLCKIV
                     KQVILGHLVVLGDVFDRGDEPDKILRILSQKDIVNYITFIWGNHDILWMGAAAGNKSL
                     IAEALRISTRYDNLDFLKRLDIDIGRLVAFAERIYPDDVTGTFKAKKPLSRKLEKTLA
                     MIQFKLEEKTIRENPELKMESRLWLEKLAGMLSAGDTEGLTDTNFPTLDLANPTELTD
                     EERAVIDDLALQFTRSIKVKALMQFLFEKGKLYHINNFIFNIHALIPSTENGEFEEFL
                     GKRGKELLDHIEDVVKTVGRNYLEDKPQDPKHLALMFYLWCGPKSPFFGKNAMKTFER
                     YFLKDKATHKERLLYWAENLKRREFMDMIMREFGSRRIVYGHTPVDILKGEKIASDDG
                     RAINIDGGFSEAYLNRGHALIHTPYSLYAIILPTPEEVIDSRKKMEPVGLLIETIDTF
                     DEPIRVRDTHKGAMLKKRREELYNYLSKYTS"
     gene            complement(3637..4125)
                     /locus_tag="PKJ16_15245"
     CDS             complement(3637..4125)
                     /locus_tag="PKJ16_15245"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HNV48398.1"
                     /translation="MSLCKVDIVNIFIDQETNAPVILLQDAETKDVLPIMIAPLEASL
                     IAIELEGKKPVRPLTHDLMANLLRELSFEVESVVIDDLKNNIYYASIHLVSGARSLEI
                     DSRPSDAIALALRTKSPIYVKKKVFALCMGLDKAAHESDKETLKQVLEDMEIDDAGGK
                     IM"
     gene            4342..4794
                     /locus_tag="PKJ16_15250"
     CDS             4342..4794
                     /locus_tag="PKJ16_15250"
                     /inference="COORDINATES: protein motif:HMM:NF015331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV48399.1"
                     /translation="MRKEYNTTQSLEIIAAPEECYRLLCDFEGYPKWFKNVRKVSITD
                     LGEDGRPLRAHYVFDIAIKQGFQIVLEYGYEDDKCRLNYKSVGGTFSDASGYYQFRRL
                     TTGKTLAEFHVHVGLGVVLPSKITSYLMDVVQVGVLKMMKNELEKDIT"
     gene            4811..5269
                     /locus_tag="PKJ16_15255"
     CDS             4811..5269
                     /locus_tag="PKJ16_15255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HNV48400.1"
                     /translation="MGKVYEATQKIKLDADAEVGYRIMCDFEKYPTWWKHVRTVNVLE
                     RDDRDVAVKVHYVFDIALKQGFKLTQHYTYDDENRVLHFNAVGGDFDKSGGFLRFTNT
                     GAGVCEVEYYVKVELSMPLAATVLFFLTEKAMKDVLLMLKTEAEKQAASG"
     gene            complement(5266..>5835)
                     /locus_tag="PKJ16_15260"
     CDS             complement(5266..>5835)
                     /locus_tag="PKJ16_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV48401.1"
                     /translation="EKKLGIRCSETREVIMDNCEVPAENLIGRAGHAFRYAIATLDKS
                     RPVVATSAVGLAQAAYEVAIKYSLEREQFGNPISSLQAIQHILAEMATRVETGRLLAY
                     TACRMCDQEHPSMATFSAMAKYQCGENAFWVANSALQIMGGYGYMRDYPIEKMLRDAR
                     ILTIYEGTSQIQLNALAHELIRSAKSGKI"
BASE COUNT         1188 a         1577 c         1804 g         1266 t
ORIGIN      
        1 ggatttttac atcctccggg aggaaaaccg ctcgcggatc gcgttctaca agaacagcat
       61 catccattac tttatcccgc tgtcgtattg cgcgctggca ttgctggcgg atgcgcggcg
      121 cggcggcgtg accaccacta cgacgcttgc gcaggagtat gcgtccatcc gcgagctcat
      181 gcgccgggag ttcgtccaca gctcggccga gggggccgac gctcccgaac gcgaggagcg
      241 cgcggcgctt caatacctga tcggcgccgg gatggttgtc gcagacggcg agacggtccg
      301 catcggcgat caggccgcgg acacgctcaa gttttatgcg aagatccttc gcgagtacct
      361 tgagtcatac tacattgtcc tgggcgcggt gctggattat cgacgcggca aggtcaatcg
      421 aaaggacttc acggtgcagg tgcgccgcaa gggaatacag ctctatcata tcggcatcgt
      481 ggggctggcc gaggcgctct cgcttcccaa ctataataac gccatcagcg tgtgcatcga
      541 caaccgcatc ctgagcgaag aggtgaccgg caaaaagacc agcaccatca cattgctcga
      601 tccgggccgg gcgcagggga tgatggaccg gatcaagaca tacctggagg tcgtggtcgg
      661 ttaagcgccg gatgtaacgg gaggtcgaat acggcgtccg gttaccacgc atactccgcc
      721 gcatcgctca tcgctctagc gccacatcat gcgtatgcgc ttctcgcaca aaaaacgggc
      781 atcgccggag ggcgatgccc gcatcatttc aagattgccg ccgacggcta caggttcagc
      841 cggagcccca ggttgagctg gttctggatg aaggtgagat tgcgcggctc cttgtaggtc
      901 gatgtatagc ggatgtactc ggcgaagaaa cgcaggaacg ggaaaaaggt aatgtcgatc
      961 ccgccgccgt agtaatacgc gccccaggcg tggcccatct cgccgcggct gccatcgttg
     1021 cccctgatgc tgaagtcggt catgtcgagc tgctcgccgg cggccgcgcc cccgcgaaca
     1081 tagggatcga tgatgaacgg aatgtcgaca agcacccgaa tgtcgggccc ataggaggtc
     1141 ttgttgacct ggtagccgtt gtagttgcgg gtatagtaaa aattgccgcg atcgaaaaaa
     1201 ccgccaatgc cgatctcaag gtcgatgacc cagaggtcca tgccggtagt gttcaggtgc
     1261 gcgacgaacc cgtattgacc gccatagctg tcgaacttgg tgacgccggg cttgtattcg
     1321 gtgtagttgc cgccgccgta tgcgccgatg tcgaagaggg cgtagcctga cacggggacg
     1381 agggcgcaca gcgcgcagat aacggctatg atgacgaact gttttatgtt cacgttcgat
     1441 cctccaaggc taaattcgat ggcgccgacg cgatcggcgg ggccaaggcg ccttaaaagc
     1501 aattaaaaat tatggtaaat aacagctttt gaaaagtcaa tgataataaa tatttttccg
     1561 accccgaatc aggtcaggaa gtatactttg aaagataatt gtatagttcc tcgcggcgtt
     1621 tcttcaacat cgcccctttg tgggtgtcgc ggacacggat cggctcgtcg aaggtgtcga
     1681 tcgtttcaat gagcaggccc accggttcca tcttcttccg ggaatcgatc acctcctcgg
     1741 gtgtcgggag gatgatggcg tagagcgagt agggcgtgtg gatgagggcg tgccccctgt
     1801 tgagatatgc ctcggaaaag cccccgtcga tgttgatggc gcggccgtcg tcggaggcga
     1861 ttttttcgcc cttgaggatg tccaccggcg tatggccgta gacgatcctc cgggagccga
     1921 attcgcgcat gatcatgtcc atgaactccc ttcgcttgag gttctcggcc caatagagca
     1981 gccgctcctt gtgcgtggcc ttgtccttca ggaaatagcg ctcgaaggtc ttcatcgcat
     2041 tcttgccgaa gaagggcgac ttggggccgc accacagata aaacatgagc gcgaggtgtt
     2101 tcgggtcctg cggtttgtcc tcaaggtagt ttcgaccaac ggtcttgacc acgtcctcga
     2161 tatggtccaa gagctccttg ccgcgtttac cgaggaactc ctcgaattcg ccgttctcgg
     2221 tgctggggat gagggcgtgg atgttgaaga tgaagttatt gatgtgatac agcttgccct
     2281 tctcgaagag gaactgcatc agcgccttca ccttgatgct ccgggtgaac tggagcgcca
     2341 ggtcgtcgat caccgcgcgc tcctcgtcgg tgagctcggt ggggttggcc aggtccagcg
     2401 tcgggaagtt cgtgtccgtg agcccctcgg tgtcgccggc ggacagcatg ccggcgagct
     2461 tttccagcca caggcgcgat tccatcttga gctcgggatt ctcgcggatg gtcttctcct
     2521 cgagcttgaa ctggatcatc gcgagcgtct tttccagctt gcgcgagagg ggctttttcg
     2581 ccttgaaggt gccggtcacg tcgtcggggt agatccgttc cgcaaaggcg accaggcggc
     2641 cgatgtcgat gtccaatcgc ttcaggaagt ccaaattgtc gtagcgggtg ctgatccgca
     2701 gcgcctcggc gatcagcgac ttgttccccg ccgccgcccc catccacagg atgtcgtggt
     2761 tgccccagat gaaggtgata tagttcacga tgtccttttg ggacaggatc cgcaggatct
     2821 tatcgggctc gtcgccccgg tcgaagacgt ccccgaggac cacgaggtgc cccaggatca
     2881 cctgcttcac gatcttgcac agcgagctga tgacgcgctc ggcgatgagg tcgttttcgt
     2941 acatgatatt gggcacgggc atgttgagaa tgaggttctc gagaaagggc cgcaggttgt
     3001 tgttgatggt gtcccggatc tggttgaagt tgatgctgtt ttggaccttg tactgtgtga
     3061 tcttcaccag ggcggatatc acgtcctggc gttccatcgt gtacgattcc gtggcgaaat
     3121 aacgttcctt gcggatgagg cggcccaggt agtcgatctt gtcgttggag aaggtcttgg
     3181 ggagcgcctc gcgacaggtt tgccacacga gcccgaagcg ccccttgagg atcgagacga
     3241 accgttcgcc ggcgccgtgg ggatcgctga tccacatcgt cgtgtcgatg aacgattcca
     3301 gctcgacctc gatctcggag atttcgtgga tgatgtcgtt cagggcggga atgtcggtcg
     3361 atttcatcag gggctcctca cggtcagtat gtccggtgtt ttcgggcatt cctgaaggca
     3421 cacaggtcca tcggaggatt catgaccatt tcggcacggg gagtgttcgc cgtgtaacgc
     3481 atggaccact cggcagtcgt cacatgcgca tcacccgccg aaatgggtgt gcataacatg
     3541 gcccgttcac atggctgacg gggagaatgg tgatacgatg accgatgcgt gtcaatgaaa
     3601 tttattcgga aacgtcggca aatcgcaggc ggcgggctac atgatctttc cgccggcgtc
     3661 gtcgatttcc atgtcctcga ggacctgctt cagggtctcc ttatcggact cgtgggcggc
     3721 cttgtcgagg cccatgcaga gggcgaacac ctttttcttc acgtagatgg gcgatttcgt
     3781 ccgcagggca agggcgatgg cgtcgctggg acgcgagtcg atctcgagcg agcgcgcgcc
     3841 ggagaccagg tggatcgagg cgtaatagat gttgttcttg aggtcgtcga tcacgaccga
     3901 ttcgacctcg aaggagagct cgcgaagaag gttcgccatg agatcgtgcg tgagcgggcg
     3961 caccggcttt ttgccctcca gttcgatggc gatgaggctc gcctcgaggg gcgcgatcat
     4021 gatggggagc acatccttgg tctcggcatc ctgcaggagg atgaccggcg cattggtctc
     4081 ctggtcgatg aagatgttta cgatgtcaac cttgcataaa gacatgtccc ttcccgcgac
     4141 aacccctggc cggggagatt ggcgcattcg gatgtcaaaa tatcaacacc tgccgtataa
     4201 agtatagcac tctttcaaaa ggacaaataa taatgtgcca gtgggcgcga tgagggcccg
     4261 gaaaaaaagc attcgaaata tgcaattgta ttgacagaaa tccatttttg gcggtacctt
     4321 tgcagcgtct ggtggcgata catgcgaaaa gaatacaata ccactcaaag tttggaaatt
     4381 attgcggcgc cggaggagtg ctaccggctt ctgtgcgatt tcgagggata cccgaagtgg
     4441 tttaagaatg tcaggaaggt ttccataacc gacctgggcg aggacggacg gccgcttcgg
     4501 gcccactacg tgttcgatat cgccatcaaa caggggttcc agatcgtgct cgagtacggc
     4561 tatgaggatg ataagtgccg gctcaattac aaatcggtcg gcggaacatt cagcgacgcc
     4621 agcggatatt atcagttcag gcggctcacc acgggcaaaa ccctcgccga gttccacgtg
     4681 catgtgggtc tgggcgtggt gcttccatcg aagatcacga gctatctcat ggacgtggtt
     4741 caggtgggcg tgctcaagat gatgaaaaac gaactcgaaa aagatataac ctagggcggg
     4801 gtgatcgcgc gtgggcaagg tatacgaggc gacgcaaaag atcaaactgg acgcggatgc
     4861 ggaggtcggg tatcgcatca tgtgcgattt cgagaagtac ccgacgtggt ggaagcacgt
     4921 acggaccgtg aatgtcctgg agcgcgacga tcgcgacgtc gccgtgaagg tccactatgt
     4981 gttcgatatc gcactcaagc agggctttaa actcacgcag cactatacct acgacgacga
     5041 aaaccgcgta ttgcacttca acgcggttgg cggcgacttc gataaatccg gcggtttttt
     5101 gcgcttcacg aacacgggcg cgggcgtgtg cgaggtcgag tactacgtaa aagtcgagct
     5161 ctccatgccg ttggccgcaa cggtgctttt cttcctaacg gaaaaggcca tgaaggacgt
     5221 tctgctgatg cttaagacgg aggccgagaa gcaggcagct tccggctaga tcttcccgct
     5281 ctttgcgctc cggatgagct cgtgtgcgag cgcgttcagc tggatctgcg acgtcccctc
     5341 gtaaatcgtt aaaatcctcg catcgcgcag catcttctcg atggggtaat cgcgcatgta
     5401 gccgtagccg cccattatct gcagcgcgct attggccacc cagaaggcgt tctcgccgca
     5461 ctgatatttc gccatggccg agaacgtggc catggacgga tgctcctgat cgcacatgcg
     5521 gcaggcggtg taggcgagga gccgcccggt ctcgacccgc gtggccatct cggccagaat
     5581 gtgctggatc gcctgcagcg agctgatggg gttcccgaac tgctcgcgct cgagcgagta
     5641 cttgatggcc acctcgtagg cggcctgcgc gagccccacg gcggaggtcg cgaccaccgg
     5701 acgcgatttg tcgagcgtgg cgatggcgta gcggaaggcg tgccccgcgc gcccgatgag
     5761 gttctcggcc gggacctcgc agttgtccat gatcacctcg cgcgtctccg agcaacggat
     5821 cccgagcttt ttctc
//
LOCUS       DAORHF010000233         5813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_13981_length_5813_cov_5.314519, whole genome shotgun sequence.
ACCESSION   DAORHF010000233 DAORHF010000000
VERSION     DAORHF010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13981_length_5813_cov_5.314519"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1408
                     /gene="mtaB"
                     /locus_tag="PKJ16_15265"
     CDS             137..1408
                     /gene="mtaB"
                     /locus_tag="PKJ16_15265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HNV48402.1"
                     /translation="MARTFSIKTLGCKLNQHESAQMASQLVAEGWTAIPFGQPADMVI
                     VNTCTVTDRSDKKCRNYIRQGAQFSRDGRVVVTGCLVARDPEGIRRMPEVSLAVSGDE
                     REHIASLVGVSPASGASSRSSNAVPLPFFRSRGSIKIQDGCDGACSYCIVPAVRGAPR
                     SMPMAEILDHARFLIDAGVPELVLTGITIGKYAYEGRSLADLARALADLPGKFRLRIT
                     SIEPNHVTDALIDVYSHEKVCQHIHLPLQSGSDRVLSAMRRPYTAAQYQGAVDSIRRA
                     APEIAVGADVIVGFPLEDDAAFNETLSLVRNISFAYVHQFTFSPRRGTEASRMPRSGG
                     AKALKRRADTLREVSLASGITYRRRFEGRIMECVVENNANRDGYTAVSGNYIKIDLEK
                     DGLDALTPGGLMRVRMLRATEAGASGCIFPV"
     gene            1710..2120
                     /gene="speD"
                     /locus_tag="PKJ16_15270"
     CDS             1710..2120
                     /gene="speD"
                     /locus_tag="PKJ16_15270"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HNV48403.1"
                     /translation="MESLGTHVIAELFNCDAYVIDDLKKVKEVFMAAAELSRATIIKP
                     FFHKFSPYGVSGVIVIAESHFTIHTWPEHGYAAVDIFTCGDLNWQDALEHIKTELKSE
                     RCSIFEMQRGLLPDHTIIKQSGVFKEVENAGCIE"
     gene            2173..2523
                     /locus_tag="PKJ16_15275"
                     /pseudo
     CDS             2173..2523
                     /locus_tag="PKJ16_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
     gene            2588..3445
                     /gene="speE"
                     /locus_tag="PKJ16_15280"
     CDS             2588..3445
                     /gene="speE"
                     /locus_tag="PKJ16_15280"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017809925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNV48404.1"
                     /translation="MSVTSELWFEEKLELDSGRTLKIRINRLIEQFESPYQRIEVYET
                     VPFGRMLVLDGVIMCTEWDEHAYHEMITHVPLFTHAKPERVLVIGGGDGGTIREVLRH
                     PSVAQADLCEIDGDVVRLSRTHLPSMACALDDPRVTIYHEDGAKFIAERPNHYDIIIV
                     DSSDPIGPAEVLFSEEFYVAMRGSLREDGIAVTQSESFYYHASIVKRLTGFAKKHYAV
                     AGYYNTIVPTYPSGIIGFTMCSKKYHPLRDFNEARVTPMQGSLRYYNPGIHRGAFDLP
                     SFIKNEINL"
     gene            3473..3916
                     /locus_tag="PKJ16_15285"
     CDS             3473..3916
                     /locus_tag="PKJ16_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48405.1"
                     /translation="MTKSELIMRRVALLYAIMFSGAVSVFIFLPGPMFDAINVVSREL
                     FPALPLASDSGKFWVALTASMMATITALSILIYRDVRKNYHLAIPIAVAKFTSSLMGL
                     AFFIGGFVAPATGWNTLANGIILLTDLPLGIVILLVYRWVDRERA"
     gene            complement(3972..4622)
                     /locus_tag="PKJ16_15290"
     CDS             complement(3972..4622)
                     /locus_tag="PKJ16_15290"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HNV48406.1"
                     /translation="MNHYDEIRTRHEITIIESVAENLRKRDFEAMVLNSNDAVRQYIE
                     SEIQPDQTVGFGGSVTVRELGLDTLLRGRGNTVFDHWDANLSPEERLSTRRRQLDADC
                     FITGSNAITRDGRIVNIDGIGNRVAAMIFGPRRVIAIVGANKIARDLDDAIWRIKNIA
                     SPRNSMRLSMDTPCARTGACVECSSRSSVCRVTTIIDYRPSETKFTVILLPVPLGF"
     gene            complement(4619..5335)
                     /locus_tag="PKJ16_15295"
     CDS             complement(4619..5335)
                     /locus_tag="PKJ16_15295"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HNV48407.1"
                     /translation="MDAVRVILCQLPLGVPITRPIADEMRSYRPHFVCFPEYFFVNRA
                     LGNHGQTPHNFNRQHHRLIELSKSLDTVIIGGTMPETVGELMYNTCFIYDRGAHIGHY
                     RKKNLFFAEVGKITPGDTFRVFTANGITFGVLICADVFHDDSFTAMRDLGARIVFIPT
                     FSLKRIETVEEKHQRDHDIFVRGSRLAGAVLVKVCGVKSPYKDFLQARSLIAGPDGVI
                     FRVRPDEEDSSIIIKQEIWT"
     gene            complement(5335..>5813)
                     /locus_tag="PKJ16_15300"
     CDS             complement(5335..>5813)
                     /locus_tag="PKJ16_15300"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV48408.1"
                     /translation="TEFGHTSIDYRGRPCPCGGRGCLERYAAAPAVVRLARRGLRTHP
                     ESSLHGLMQAKPLSAALLYQAARGGDRFAMHVFETTGEFLGIGIAAFVNIFNPEAVVI
                     GGGLSRAHRFFMPSLRTALARHALPAFRAGVRFHMARDVVRGAALGAAKLAVDRFS"
BASE COUNT         1190 a         1640 c         1742 g         1241 t
ORIGIN      
        1 gcagggcggg atgtgcggat cgccatttcc cgccgattct gcgacatccc ggcgcgtttc
       61 cgcgcggtgg ccgtttttca tacccattgg gcatatttac tattgacatc atcagttgcc
      121 gccagcaaca gggtgcatgg cgcgaacatt ctcgataaaa acgctcggat gcaagctcaa
      181 ccagcacgaa tcggcgcaga tggcctcgca gctcgtcgct gagggctgga cggcgatacc
      241 attcggccag cctgcggata tggtcatcgt caatacctgc acggtgaccg accgaagcga
      301 taaaaaatgc cgcaactata tccggcaggg ggcgcagttt tcccgggacg ggcgcgtggt
      361 ggtaaccggc tgccttgtcg cgcgagatcc ggaggggatt cgccggatgc cggaggtttc
      421 actggcggtt tccggcgacg aacgcgagca tatcgcgtcc ctcgtgggcg tatcgcccgc
      481 gtccggtgct tcgtcgcgat catccaatgc cgttcccctg ccgtttttcc gctcccgcgg
      541 ctccattaaa atccaggacg ggtgcgacgg cgcctgcagc tattgcatcg ttccggcggt
      601 ccgcggggct ccccgaagca tgccgatggc ggaaattctc gaccacgcgc gattcttgat
      661 cgacgccggc gttccggagc tcgtgcttac cggcatcacc atcgggaaat acgcatatga
      721 gggacgctcg cttgccgatt tggcccgggc cctggcggac cttcccggaa aattccgtct
      781 tcggatcacg tccatagagc caaatcatgt caccgatgcc cttatcgacg tgtattccca
      841 cgaaaaggtg tgtcagcaca tccatttgcc gctgcaatcc ggatccgatc gcgtcctctc
      901 ggccatgcgg aggccgtaca cggccgctca gtaccaggga gcggtcgatt ctatcaggcg
      961 cgcggcgccc gagattgccg ttggggccga cgtgatcgtc ggttttcctc tcgaggatga
     1021 tgcagcgttc aacgaaacgc tgtccctggt gagaaatatt tcgtttgcat atgtccatca
     1081 gttcacattt tcgccccggc ggggcaccga ggcatcacgc atgcctcgct cgggaggcgc
     1141 gaaggcgctc aaacggcgtg ccgacaccct ccgggaggtc tcactcgcgt ccggaatcac
     1201 gtatcgacgc cgtttcgagg gcaggatcat ggaatgcgtt gtcgaaaaca acgccaatcg
     1261 cgacggctat acggccgtga gcggcaatta tatcaagatc gatctcgaga aggacggtct
     1321 cgacgcgctc acgccaggcg gtctcatgcg cgttcggatg ctccgggcaa ccgaagccgg
     1381 ggcctccgga tgcatttttc ctgtgtgaca taattttttt atgaattttg ataaaatatt
     1441 acttgctata ttgtaaaaac attttaatgc agtcgtaaat ttatcgcgac actatcctat
     1501 actggaccat cggggggtga tactttaatc cgcacgcacc gaacgagtga tttattttct
     1561 tgacatatag cgctgatatt ttttttgttc acagtctttt gaacgaaggt acgaagaggt
     1621 aaccggctga tgcaggtact cgttttaaaa tatcttttta ccctgcatcc atgcgggtgc
     1681 ggaaaatatt aatgtcaagg ggtgctgcca tggagagtct ggggacacac gtaattgcgg
     1741 aactctttaa ctgcgatgca tatgtcatcg acgatctcaa gaaggttaag gaggtattca
     1801 tggccgcagc cgaactctct agagccacca ttataaagcc gttcttccac aaattctcgc
     1861 cgtacggcgt cagtggcgtc atcgtcatcg ccgaatccca tttcaccatt cacacgtggc
     1921 ctgagcatgg ctacgccgcg gtggacatct tcacctgcgg cgacctcaac tggcaggacg
     1981 cgctggaaca catcaaaacc gagctcaagt cggagcggtg ctccattttc gagatgcagc
     2041 ggggcctgct gccggatcat acaatcatta agcaatcagg tgttttcaag gaggttgaaa
     2101 atgcaggctg tattgaatag caaggatatc gacgaaataa ttacgccatc gccggaacag
     2161 tatgggaccc tcggcgcctg gggattgtac tcgagcgtcg acatccataa ctgcgatccg
     2221 gacatcattc gcagctcgga gatgattcat cggtatgtcg tcgagctctg cgaactcatc
     2281 gagatgcgtc gctttggcga ggccttgatc gtgaatttcg gcgaggacga gcgtgtggcc
     2341 ggctattcga tggtgcagct tatcgaaacc tcctgtatct ccggtcactt cgcgaatctg
     2401 accaacacgt cgtacatcga tgtgttcagc tgcaagtggt atgatccgga ggtggtggcg
     2461 aaattttcgc tcaatttctt caaggggacc gactacaatc tgcacgtcaa tgtgcgaaag
     2521 tagcgggtca cggtcgaaag ccccacgcgg ctgcgtgggg ctttctgcat tatagggggg
     2581 ttgcgcgatg tctgttacgt ctgaactatg gtttgaagag aagcttgagc tcgattccgg
     2641 tcggacgctc aagatccgca tcaatcggct gatcgagcag ttcgaatcgc cgtaccagcg
     2701 tatcgaggtg tacgagaccg ttccgttcgg gcgcatgctc gtgctggacg gcgttatcat
     2761 gtgcaccgaa tgggacgagc acgcctatca tgagatgatt acgcacgttc ccctgttcac
     2821 ccacgcgaag ccggagcggg tgctcgtcat cggcggcggc gacgggggga cgatccgcga
     2881 ggtattgcgc catccgtcgg tggcacaggc cgacctctgc gagatcgacg gcgatgtggt
     2941 gcggctgtcg cggacgcatc ttccgtcgat ggcatgcgcg ctggatgatc cgcgcgtcac
     3001 gatctaccat gaggacgggg cgaagttcat cgccgagcgc ccgaaccact acgacatcat
     3061 catcgtggat tcctccgatc ccattggacc ggccgaggtg ctcttctccg aggagttcta
     3121 cgtcgccatg cgcggttcgc tcagggagga cggcatcgcc gtcacgcagt cggagtcatt
     3181 ttattatcat gcgagcatcg tgaagagact caccgggttc gcgaaaaagc actatgcggt
     3241 ggccggctat tacaatacca tcgtgcccac ctatccgagc ggcatcatcg gatttacgat
     3301 gtgctcgaag aaatatcatc ccctgcgcga ttttaacgag gcgcgcgtga cgcccatgca
     3361 gggctcgctg cgatactaca atccggggat ccatcgtggt gcgttcgacc tgccgagctt
     3421 cataaaaaac gagataaacc tgtaacgttt gttcggggcc tggaggggaa tggtgacgaa
     3481 atcggaactg atcatgcggc gcgtcgcgct tctgtatgca atcatgttct ccggggcggt
     3541 gtccgtattc attttccttc ccggacccat gtttgatgcg attaacgtcg tgtcccgcga
     3601 gctgttcccc gcgctgcccc ttgcgtcgga cagcggaaaa ttctgggtgg ccctcacggc
     3661 gtccatgatg gcgaccatta cggcgctctc gatactgatc taccgtgatg tacggaaaaa
     3721 ctatcatctc gccattccca ttgccgttgc gaagttcacg tcttcgctga tgggcctcgc
     3781 ttttttcatc ggcgggtttg tcgcgccggc caccggctgg aataccctgg caaacggcat
     3841 catcctgctc accgaccttc ccctggggat cgtcatactc ctcgtatacc gctgggtgga
     3901 tcgcgagcgc gcctgagggc cgctgtgacg aaacgtgcgc aaagagtgcg ctctgacggc
     3961 gttggtttag tctaaaaacc aagcgggacg ggcagcaaaa tcacggtaaa cttggtttcg
     4021 gacggtcgat aatcgatgat cgtcgtgacg cggcacacgg agcttcgcga cgagcactcg
     4081 acgcaggcgc cggtccgcgc gcacggcgtg tccatgctca accgcatcga gttgcgcggc
     4141 gaggcgatgt tcttgatgcg ccagatcgcg tcgtcgaggt cgcgggcgat tttattggcg
     4201 ccgacgatgg cgatgacgcg ccgcggaccg aagatcatgg cggccacccg gttcccgatc
     4261 ccatcgatgt tgacgatccg cccatcgcgc gtgatggcat tgctccccgt gatgaagcag
     4321 tcggcgtcga gctggcggcg gcgcgtcgag agccgttctt ccggtgaaag gttcgcgtcc
     4381 cagtggtcaa agacggtatt accgcgaccg cggaggagcg tatcgaggcc cagctcgcgt
     4441 acggtaaccg accccccgaa ccccacggtc tgatccggtt gaatttccga ctcgatatac
     4501 tgccgcaccg cgtcattgga gttgagcacc atggcttcga aatcgcgctt ccggagattt
     4561 tcggcaaccg attcaattat tgtgatctca tgccgagttc gaatctcgtc atagtgattc
     4621 atgtccaaat ctcctgtttg atgatgatcg aggagtcctc ctcgtcgggt cgtacgcgga
     4681 agatcacgcc gtcgggccca gcgatgaggc tgcgggcctg gagaaagtcc ttatagggcg
     4741 atttcacgcc gcacaccttc accaggacgg cgccggcgag gcgggacccg cgaacgaaga
     4801 tgtcgtggtc gcgctggtgc ttttcctcga ccgtttcgat ccgcttgagc gagaaggtcg
     4861 gtatgaacac gatgcgggcg ccaagatcgc gcatggcggt aaaactgtcg tcgtgaaaga
     4921 catcggcgca gataagaacg ccgaaggtaa ttccgttcgc ggtgaaaacg cgaaacgtgt
     4981 cgccgggagt gatcttcccg acctcggcga aaaagaggtt ttttttgcgg taatggccga
     5041 tgtgcgcgcc ccgatcatag ataaagcagg tgttgtacat caactccccg acggtctccg
     5101 gcatcgtacc gccgatgatc accgtatcga gcgatttcga gagctcgatg agccgatgat
     5161 gctggcgatt gaaattgtgc ggggtttggc catgattgcc cagcgcacgg ttcacgaaaa
     5221 aatactcggg gaagcagacg aaatgcgggc ggtatgagcg catctcgtcc gcgatcgggc
     5281 gggtgatggg aacacccagc ggcagttgac agaggataac gcgaacggcg tccatcacga
     5341 gaaccggtcc actgcgagct tcgcggcgcc gagcgcggcc ccgcgcacga cgtcacgggc
     5401 catgtgaaat cgcacaccgg cgcgaaaagc ggggagggcg tgtcgagcga gcgccgttct
     5461 cagggagggc atgaaaaatc gatgggctcg ggaaagacct ccgccgatca caaccgcctc
     5521 cgggttgaag atgttcacga aggcggcgat accgatcccc agaaactcgc cggtcgtttc
     5581 gaaaacatgc atggcgaacc tgtcgccgcc gcgagcggct tgatacagga gcgcggccga
     5641 gaggggtttg gcctgcatca agccgtggag cgacgattcg ggatgcgtgc gcaacccgcg
     5701 ccgtgcgagg cggacaacgg cgggcgcggc ggcatagcgc tcgaggcagc cgcgcccacc
     5761 gcatgggcac gggcgaccgc gatagtcgat cgaggtgtgg ccaaattcgg tca
//
LOCUS       DAORHF010000234         5806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14008_length_5806_cov_3.823509, whole genome shotgun sequence.
ACCESSION   DAORHF010000234 DAORHF010000000
VERSION     DAORHF010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5806
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14008_length_5806_cov_3.823509"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="PKJ16_15305"
     CDS             complement(<1..505)
                     /locus_tag="PKJ16_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48409.1"
                     /translation="MKINIVRSLAIVFIVGLLFFLVLENLRKPRVFILHSYALDYSWV
                     RDINDGIMRIFKKRPYSLRWHYMDTKRHPAEDFKRHAGNTARRLIKNWRPDIIIAIDD
                     TAQEHVAKYFNNDPAINIVFTGVNAETKTYGYDTASNATGMLERVPLKEFKEVFVSLL
                     PKDKRRIV"
     gene            complement(502..2349)
                     /locus_tag="PKJ16_15310"
     CDS             complement(502..2349)
                     /locus_tag="PKJ16_15310"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HNV48410.1"
                     /translation="MDAKMQRLVQDLKSADIFASCSATDIARIIPYLQERTLRAGDVL
                     FEAGSPATTFYLVSSGTVALLTGKKTTQEISTGTIGEESVYEERYLSDAIAVTDATVY
                     AIDREHLRYVLGSGAGFMQALYRSIINHHVVRSLAGALRPTRVEAAPERPDRLKIIGW
                     VLAIALPVVILLAGGGWGMDWKTKIFATVAVSTIVMWIFRLAHEFIPSIFAVLVILIL
                     DIAPPEVALSGFTSGSFFMALSIFGLSAVLVSSGLTYRLVVTLLRFVPRSKFWYNMTI
                     MLVGIFLTPLMPSANGRISLANPILVDMVDSLGYRKGGRAATDLSVATFSGFTLFSGI
                     FLSSKSIHFVIFGLLPIQVQEQFNRGYWLYASLVVGVVLLALHVVVSKYLFANDEPTN
                     LSRERVETQQQVLGPMSTHEWAALGGIVLFLLGIATSTIHKIDLSWIGLTVLYVVLAL
                     EFLSKKEFTGSIDWTFLIFLGTLIGLVKSMSYIGLDAGIGKHLLWFGMYTRTNFALFV
                     LLLGASILVLRFFIPNNATVAILAAIFLPIAEISGVNPWVVGYIILFMSDAWLLPYQC
                     TYYLQFEELAHKDGIYDSRLFLKYNAISVLFRFAAVYASIPFWKYLGIL"
     gene            2772..3416
                     /locus_tag="PKJ16_15315"
     CDS             2772..3416
                     /locus_tag="PKJ16_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48411.1"
                     /translation="MSIRSFESFVRCVIIAALASAITPASLYGTGTRMENRAHITITR
                     GQSPWAGMTESFAQEQANKRISAVAIDPAVFTPLPHLAAYAIGSDPLMGRSAATKRVV
                     VSSAYFSDLERADATRIAAGATKRIGRALPAAISKLGPERLVLFLIEAQIIETYWHIE
                     AKLRLVGEQKTAHAYTARFVGTHIYFTNERNEDPLDFAVSIDLKTGEMGISGSK"
     gene            complement(3460..4296)
                     /locus_tag="PKJ16_15320"
     CDS             complement(3460..4296)
                     /locus_tag="PKJ16_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48412.1"
                     /translation="MELKVVLAITLSVLFFTIINLKSKFFSRELLIVAGFTVFISLAA
                     NKDNPDFSLVRILVAIPLCFTGGIAVFFRDEILPRITERSLHLLSLLHGYTVLVNVPV
                     AQTPVMAMLLMAPVVVAHYYAISRRALPASEKIFAYCVQLYFLVCIGYYNLKRMVLYD
                     MPPGVIVQVDVELFVRLFCISGMLFYILIYVLNLLLAVPLPAKCEPDYSRLFIEKMGD
                     AQANPAVTLAITAMAAALFGLNLRYHAIPHSFITEFLVVCVVIGAPHVDAIAARIAGM
                     RS"
     gene            complement(4525..>5806)
                     /locus_tag="PKJ16_15325"
     CDS             complement(4525..>5806)
                     /locus_tag="PKJ16_15325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015906507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV48413.1"
                     /translation="GADIYSSDMMLYPNPDGTYRANGDKYYIGNGNEAAMVSTFGKIA
                     DSGDYVFFAVNSKHPKYECVKNIINNQDYVAEYALHDYPITEADILEKGQKAWDDMLN
                     TINICKFNLGWGSIGICEHALYESLNHAAHRNLFGSYVIDFTHIKQLFVDAYCRLVAM
                     KLFALRATDYMRSANEDDKRYLLYNPMVKMKVSTQGEEVINLLWDVIAAKGFEKDTYF
                     ENAATDIRALPKLEGTVHVNMALIIKFMPNYFFNPKEYAPVPQRRDAANDDFLFNQGP
                     TKGLGKLQFHDFNIAYNSVNIPNVNVFKEQIEAFKEFMLASMETMKEQANDIDFLLQV
                     GECFTLVAYGQLILENKKIYDVSDELIDQIFDFMIRDMVKFAMQLYAKPIASEKQQAA
                     CLKMIKRPVVNKERYDRICKDYVYSLIDTYTMNE"
BASE COUNT         1292 a         1691 c         1715 g         1108 t
ORIGIN      
        1 gcacgatgcg ccgcttgtcc ttgggcaaga gcgagacgaa cacctccttg aactccttga
       61 gcggcacgcg ctccagcatc ccggtcgcgt tggacgcggt gtcgtagcca taggtcttgg
      121 tctccgcatt cacgccggta aagacgatgt tgatggcggg gtcgttgttg aagtacttcg
      181 cgacatgctc ctgggccgtg tcgtcgatgg cgatgatgat gtccggacgc cagttcttga
      241 tgaggcgccg ggccgtgttc cccgcgtgcc gcttgaagtc ctcggcggga tggcgcttgg
      301 tgtccatgta gtgccagcgg agggagtacg gccgcttctt gaatatccgc atgatcccat
      361 cgttgatgtc gcggacccac gaataatcga gcgcgtagct gtgcaggatg aacacccggg
      421 gctttcgcag gttctcaagc accaggaaaa acagcaggcc cacgatgaac acgatggcga
      481 ggcttctgac gatattgatc ttcataatat acccagatat ttccagaacg gtatggacgc
      541 gtatacggcc gcgaaccgga agagcacgct gatcgcgttg tatttcagga agagccgcga
      601 atcgtagatc ccgtccttgt gggcaagctc ctcgaattgc agatagtagg tgcactggta
      661 cggcagcagc caggcgtcgc tcatgaacag gatgatgtac ccgaccaccc agggattgac
      721 gccgctgatc tccgcgatcg gcaggaatat cgccgcgagg atggcgaccg tcgcgttgtt
      781 cgggatgaag aagcgcagga ccaaaatgct cgcccccagg aggagcacga agagcgcgaa
      841 gttcgtccgg gtgtacatcc cgaaccagag caggtgcttc ccgattcccg cgtccagccc
      901 gatgtacgac attgacttga ccaggccgat gagcgttccg aggaagatga ggaaggtcca
      961 gtcgatgctg ccggtgaact cctttttcga aaggaactcg agcgccagca ccacatagag
     1021 gaccgtgagc ccgatccacg acaggtcgat tttgtggatc gtcgatgtcg cgatccccag
     1081 gaggaaaagg acgatcccgc ccagggcggc ccactcgtgc gtgctcatgg ggcccaggac
     1141 ctgctgctgc gtctccaccc gctcgcgcga caggttcgtc ggctcgtcgt tggcgaagag
     1201 gtattttgaa acgaccacgt gcagcgccag cagaacgacg cccaccacca gcgacgcgta
     1261 gagccagtat ccccggttga actgctcctg cacctggatg ggcagcaggc cgaaaatgac
     1321 gaaatggatc gacttgctgc tcaggaatat tcccgagaag agcgtgaagc cggagaacgt
     1381 cgccaccgac agatcggtcg cggcgcgtcc gcccttccgg tagcccagcg aatcgaccat
     1441 gtccaccaga atcgggttcg cgaggctgat tcggccgttg gccgacggca tcagcggcgt
     1501 aaggaagatc cccacgagca tgatggtcat attgtaccag aatttggagc ggggaacaaa
     1561 ccggagcagg gtgacgacca gccgatacgt gagccccgat gagaccagca ccgccgaaag
     1621 cccgaagatg ctcaaggcca tgaagaagct tcccgaggtg aaccccgaga gcgcgacctc
     1681 cggcggggcg atgtcgagga tcagaatgac cagcaccgcg aagatgctgg ggatgaactc
     1741 gtgcgcgagg cggaagatcc acatcacgat ggtgctcacc gcgacggtcg cgaatatctt
     1801 cgtcttccag tccatccccc agccgccgcc ggcgagcagg atgaccaccg gcagcgcgat
     1861 cgccagcacc cagccgataa tcttcaaccg gtccggccgc tccggggccg cctcgacgcg
     1921 cgtggggcgc agggcgccgg caagcgagcg gacgacgtgg tgattgatga tggaacgata
     1981 cagcgcctgc ataaaccctg cgccggaacc cagcacgtac cgaaggtgct cccggtcgat
     2041 cgcatagacc gtcgcgtccg ttacggcgat cgcgtcggag agatagcgct cctcgtacac
     2101 ggactcctcg ccgatggtcc cggtggaaat ctcttgtgtc gtctttttcc cggtcaggag
     2161 ggcgacggtc ccgctcgaaa ccagatagaa cgtggttgcc ggggacccgg cctcgaacaa
     2221 tacgtcgccg gcgcggagcg tccgctcctg aaggtagggg atgatgcggg cgatatcggt
     2281 cgcgctgcac gatgcgaaga tgtccgcgct cttcaggtcc tgcacaagcc tctgcatttt
     2341 cgcgtccatg ccgcctcccg gtcctgctct ctaaccgata gtacgatgta cccgagcgca
     2401 tatgttcggg caacaacttt tcatcgaaaa aaaatatgtc ggttttcaat gagacataat
     2461 taaaaacgct tgataataat tatgtcgcat cgacatagtg cgggaaatat caatcaggag
     2521 gcctccgatg cccaagtgca gcacctctaa aaaaaccacc aaaaaaccgg ccgccaagaa
     2581 gaccgtgaaa aaggccgccg cgaaacccaa agcgaagaag aaataacacc gcttcgccgg
     2641 catactgccc cgtgcaaccg gcggggtagt atgcgacaat cgcccgcgcg cgccctcccg
     2701 cttcgttctt tttcgattgc cttttttccg atactgtcgc aggattaatc aatacaacac
     2761 gggggccggc catgtccatt cgatccttcg aatcattcgt gcgctgcgtc atcatcgccg
     2821 cgcttgcatc agccattacg ccggcatccc tttatggaac gggcacccgc atggaaaaca
     2881 gagcgcacat cacgatcacc aggggtcaga gcccctgggc cggcatgacc gagagcttcg
     2941 cgcaggaaca ggcgaacaag cggatatcgg ccgttgcgat cgacccggcc gtcttcacgc
     3001 cccttccaca cctcgccgcc tatgcaatag gctctgaccc cctgatgggt cgaagcgccg
     3061 cgacaaagcg cgtggtggtg tcgagcgcgt atttctcgga cctggagcgg gcggacgcta
     3121 cgcgcatcgc cgcaggagcc acgaagcgca tcgggcgcgc gctcccggcg gcgatttcga
     3181 agctgggccc ggagcgactg gtcttatttt taattgaggc tcaaatcatc gagacctact
     3241 ggcatatcga ggcgaagctg cgcctggtcg gggagcagaa aaccgcgcat gcgtacaccg
     3301 cgcgctttgt cggaacccac atctacttca ccaacgaacg gaacgaggac ccgctcgact
     3361 tcgccgtcag catcgacctc aaaacgggcg agatggggat ttcgggatcg aagtagtcct
     3421 gatggcgcgc ccgtaccggg tgatcaggct ggcgaacgat cacgatcgca ttcccgcaat
     3481 tcgagccgcg atcgcatcga catgcggcgc accgatgacg acacacacca ccagaaactc
     3541 cgtgatgaac gaatgcggta tcgcatgata gcgcaggttg agcccgaaca gagcggcggc
     3601 catcgccgtt atggcaagcg tgacggccgg attcgcctgc gcgtccccca tcttttcgat
     3661 gaacagcctc gaatagtcgg gctcgcactt cgccgggagc ggcaccgcca gcagcagatt
     3721 gaggacatag atgagaatat agaagagcat gccggaaatg caaaacagcc tgacgaacag
     3781 ttcgacatcc acctgtacga taacgccggg cggcatatca tacagcacca tccgtttcag
     3841 gttatagtag ccgatgcaga ccagaaagta cagctgcacg caatatgcga atatcttttc
     3901 cgatgccggc agggcccgac gcgagatcgc gtaatagtgc gcaacgacga ccggcgccat
     3961 gagcagcatc gccatgaccg gcgtctgcgc caccggcacg ttgacgagca ccgtgtagcc
     4021 atgcagcagc gagagcaggt gcagcgaacg ttcggtgata cgcggcagga tctcgtcgcg
     4081 gaagaacacg gcgatcccgc cggtaaagca gagcgggatc gcgaccagga tcctcaccag
     4141 agagaaatcc gggttgtcct tgttcgctgc gagggagatg aagaccgtaa acccggcgac
     4201 gatgagaagc tcgcgtgaaa aaaacttact cttcaggttg ataatcgtaa aaaacaagac
     4261 cgacagggtt attgccagaa caaccttcag ttccatacca gcctttaaat gaaaaggtca
     4321 caatcggccc gattcgacac gccacggata cgaaccgtaa cgccatagag aagagtgacg
     4381 catgcaaaaa agtcaagcga ttcacgaaat cgcccacctg ccccttcccg cctcggatcg
     4441 cgcacaaaaa aagaagccgg caggggatgc cggctaattg ggtaacattg tcaaaagctt
     4501 gtactgacaa gtgcgagtat gcgatcactc gttcatcgtg taggtgtcga tgagcgaata
     4561 gacgtagtcc ttgcagatgc ggtcgtagcg ctccttgttc accaccgggc gcttgatcat
     4621 cttgaggcac gcggcctgtt gcttctcgga ggcgatgggc ttcgcgtaga gctgcatggc
     4681 gaatttcacc atgtcgcgta tcataaaatc gaagatctgg tcgataagct cgtccgacac
     4741 atcgtagatc ttcttgttct cgagaatgag ctgcccgtag gcgaccagcg tgaagcactc
     4801 gcccacctgc aggaggaagt cgatgtcgtt ggcctgctcc ttcatcgtct ccatcgacgc
     4861 gagcatgaac tccttgaagg cctcgatctg ctccttgaag acgttcacgt tggggatatt
     4921 cacgctgttg taggcgatgt taaaatcgtg gaactgcagc ttgcccagcc ccttcgtggg
     4981 cccctggttg aacagaaaat cgtcgttggc cgcgtcgcgc cgctgcggaa cgggcgcgta
     5041 ctccttcgga ttaaagaaat agttgggcat gaacttgatg atgagcgcca tgttcacgtg
     5101 caccgtcccc tccagcttgg ggagggcccg gatgtcggtc gcggcgttct cgaagtaggt
     5161 gtccttctcg aaacccttgg ccgcgatcac gtcccacagc aggttgatca cctcttcgcc
     5221 ctgcgtggag accttcatct tcaccatggg attgtacagg agatagcgct tgtcgtcctc
     5281 gttcgcggaa cgcatgtagt cggtcgcgcg caacgcgaag agcttcatcg cgaccagccg
     5341 gcagtaggcg tccacgaaga gctgcttgat gtgcgtgaaa tcgatcacat agctcccgaa
     5401 gagattgcgg tgcgccgcgt gattgagcga ttcgtacagg gcgtgctcgc agatcccgat
     5461 ggagccccac cccagattga acttgcagat gttgatggtg ttgagcatgt cgtcccaggc
     5521 cttctgcccc ttttcgagga tatcggcctc ggtgatgggg tagtcatgca gggcgtactc
     5581 cgcaacgtag tcctggttgt tgatgatgtt cttcacgcac tcgtatttcg ggtgcttgga
     5641 attcacggca aagaagacgt aatcgccgct gtcggcgatc ttgccgaagg tggagaccat
     5701 ggccgcctcg ttaccgttgc cgatgtagta cttgtcgcca ttggcgcggt aggtgccgtc
     5761 cgggttgggg tacagcatca tgtcgctgga gtagatatcg gcccca
//
LOCUS       DAORHF010000235         5792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14060_length_5792_cov_4.500261, whole genome shotgun sequence.
ACCESSION   DAORHF010000235 DAORHF010000000
VERSION     DAORHF010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14060_length_5792_cov_4.500261"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /gene="carB"
                     /locus_tag="PKJ16_15330"
     CDS             <1..1589
                     /gene="carB"
                     /locus_tag="PKJ16_15330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV48414.1"
                     /translation="YNAPDAVGSSSNKKIIVLGGGPNRIGQGIEFDYCCVHAAMEIRE
                     MGYDSIMVNCNPETVSTDFDTSNKLYFEPLTVEDVLAICEKEKPDGVIVQFGGQTPLN
                     IAGELKRNGVTIIGTSPDSIDLAEDRDRFRQMIRDIGIPQPDSGMASTVEEALAVAEK
                     IGYPLIVRPSYVLGGRAMEIVHTGEMLRRYMQAAVDVSPERPILIERFLDNAIEAESD
                     ALCDGTDAFVPAVMEHIELAGVHSGDSACVIPPFSIPPKHIETIKDYTRRIAIELDVV
                     GLMNIQYAIYNDTVYILEANPRASRTVPLVSKVCNIQMAKIATQLMLGGRLADFNLVD
                     TPVRHFGVKESVFPFNMFPEVDPVLGPEMRSTGEVLGIAPSFGLAFFKAQEAAKQILP
                     FSGAVLITVTDKDKPQAVYVAREFKRLGFAIMATEGTHAYLREQGVEATLVVKLNQGR
                     PDMVDRVKNGDIALIVNTPMSKQANFDDSYIRKAAVKYKIPYITTMTAAIATAKGIEA
                     RRGGSTTVKSLQEYHAEIGR"
     gene            1899..2402
                     /locus_tag="PKJ16_15335"
     CDS             1899..2402
                     /locus_tag="PKJ16_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNV48415.1"
                     /translation="MGVLGVGYADPVASVVANHAVMPVPFVSSIPSTHTTPDLSLRNK
                     IVETAKKYIGVRYNFGGMSEKGFDCSGFVNRVYRENGIALPRTSKAQFSNGKKVSLKE
                     AKPGDIVYFVRYPRRRATHVGIYLGDSQFIHAPSRGKRVSISNIGQRYWRHRLIGVVS
                     YLPKNSE"
     gene            complement(2411..3178)
                     /locus_tag="PKJ16_15340"
     CDS             complement(2411..3178)
                     /locus_tag="PKJ16_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005827418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNV48416.1"
                     /translation="MKCETVHALVTGGASGLGEAAVRSIVKGGGRAAILDMADDKGSA
                     LAKELGNAAIFHKTDVASENDVSAAIQAAVSAFGSVNAVVNCAGIATAAKTVSGKGPF
                     PLDLFELTLRVNLIGTFNVIRLAAAQMAKNAPTPDGERGVIINTASVAAFDGQVGQAA
                     YSASKAGIVGMTLPIARDLAAYGIRNNTIAPGIFETPMATLMPDSLKQSLISQVPFPS
                     RFGRPEEFAGLVVHIIENAFINGETIRIDGAVRMAPK"
     gene            complement(3186..3455)
                     /locus_tag="PKJ16_15345"
     CDS             complement(3186..3455)
                     /locus_tag="PKJ16_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48417.1"
                     /translation="MNRTAIAILSLTALVLFSPALRADDAAAPADAETECIKKCYTDK
                     KACMDEVQDIKKKTPGQVGRMRYKTKTCNSTFAQCRNECKSKKAK"
     gene            complement(3656..3961)
                     /locus_tag="PKJ16_15350"
     CDS             complement(3656..3961)
                     /locus_tag="PKJ16_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48418.1"
                     /translation="MKKFLVVLVTVAVAAMFINVADAKKHKKGTPVDKECIKKCAADM
                     KACNAEVKKLKGKEKRAKAKECKKAAKDCKKACVKPAADKPADKPADKPVDQPAEGK"
     gene            complement(4071..5015)
                     /locus_tag="PKJ16_15355"
     CDS             complement(4071..5015)
                     /locus_tag="PKJ16_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48419.1"
                     /translation="MKQTAHIRDAELLDLFDGAIPAARSRAMRTHAADCPRCHARLAA
                     AETLLAASDNAALAPSDAVLRRVRASYRRRAGAPRRLDRVRDFARERRSLAIASALAA
                     ALILLATTTLLVRQGENGGQVTMDISYIEGDVYINNAEADLTSKIKPNSRIRVGKDSL
                     IEFSNTSAYIIKAIDDSVLVFERAKTPGVRGRRAYTIRLIRGTILSQMRKNTLGLEYS
                     YSTPEAYLRPLEGEFILQSAGDRTVVIMKSGSMRIKSLSSDERVTAEPNKKYTITKSI
                     DRGDAEDDDLDDFNINDAIHRQDGQREPADASTGTVNG"
     gene            complement(5040..5618)
                     /locus_tag="PKJ16_15360"
     CDS             complement(5040..5618)
                     /locus_tag="PKJ16_15360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48420.1"
                     /translation="MLDEEKLIALYRRHSRELFVFIVRYTGSHEAAEDILHDCFENLM
                     SYAARYPITDDNLRAFLFTTARNLCVNRHRSRVRRGPRPLDESAPDRRTAAPDALSES
                     RELAALIATLVEGLDEPDRSIFLMKKELGLTYPEIARATGHSERTVRRRLSETLDRMR
                     DALHAAGFLAFFVFLPFFVAAYCATIVTHAWR"
BASE COUNT         1051 a         1762 c         1807 g         1172 t
ORIGIN      
        1 cctacaatgc gccggacgcc gtggggtcct cgtccaacaa gaagatcatc gtgctgggcg
       61 gcggtcccaa ccggatcggc caggggatcg agttcgacta ctgctgcgtg cacgcggcga
      121 tggagatcag ggagatgggg tacgactcca tcatggtcaa ctgcaatccc gagaccgtgt
      181 ccaccgactt cgacacatca aacaaactct acttcgagcc gctcaccgtt gaggacgtcc
      241 tggccatctg cgaaaaagag aaaccggacg gggtgatcgt gcagttcggc gggcagaccc
      301 cgctgaatat cgccggcgag ctcaagcgca acggcgtcac gatcatcggc acctcgcccg
      361 actcgatcga cctggcggag gaccgcgacc gtttccggca gatgattcgc gatatcggca
      421 tcccgcagcc cgactcgggg atggcgagca ccgtcgagga ggcgctcgcg gtggccgaga
      481 agatcggcta tccgctcatc gtgcggccct cgtatgtcct cggcggccgg gcgatggaga
      541 tcgtccacac cggcgagatg ctgcgccgct acatgcaggc cgcggtggac gtctcgcccg
      601 agcgccccat cctcatcgag cgcttcctgg acaacgccat cgaggcggaa tccgacgccc
      661 tctgcgacgg caccgacgcc ttcgtgccgg cggtgatgga gcacatcgag ctcgcgggcg
      721 tgcactccgg cgattccgcg tgcgtgatcc cgccgtttag catcccgccc aagcacatcg
      781 agaccatcaa ggactacaca cgccgcatcg ccatcgagct tgacgtggtg ggcctcatga
      841 acatccagta cgccatctat aatgatacgg tgtatatcct cgaggcgaac ccgcgcgcgt
      901 cgcgcaccgt gccgctggta tccaaggtgt gcaacattca gatggcgaag atcgccacgc
      961 agctcatgct gggcgggcgg ctcgccgatt tcaacctggt cgatacgccg gtgcgacact
     1021 tcggcgtgaa ggagtcggta tttcctttca acatgttccc ggaggtggac ccggtgttgg
     1081 gtcccgagat gcgctcaacc ggcgaggtgc tggggatcgc gccgtccttc ggtttggcct
     1141 tcttcaaggc gcaggaggcg gccaagcaga tccttccctt ttcgggggcg gtcctcatca
     1201 ccgtgaccga caaggacaag ccgcaggcgg tctacgtggc gcgggagttc aaacgcctgg
     1261 ggttcgcgat catggccacc gagggtacgc atgcctacct gcgggagcag ggcgtggagg
     1321 cgacgctggt cgtgaagctc aaccaggggc ggcccgacat ggtcgatcgc gtgaagaacg
     1381 gggacatcgc gctcatcgtt aacaccccca tgagcaagca ggccaatttc gacgattcgt
     1441 acatccgcaa ggccgccgtc aagtacaaga tcccctacat cacgacgatg actgcggcca
     1501 tcgcaaccgc gaaggggatc gaggcgcgcc gcggcgggag caccacggtg aaatcgctcc
     1561 aggagtacca tgcggagatt gggcgatagc gtgtaacagg catgtcgccc tgtccgtgaa
     1621 gccccatcgg cgcgaagcgc gtcatggggc ggacggggca tgatggggtt atgggagacc
     1681 atgcccgtgc atttcggagc gattaacgcc actgtgtcca tcggcgcctc gatcgtggcg
     1741 cccgaagcat cgtattttta tagacaaatc aagaaatcga catagaatca tttttttaaa
     1801 aaaaacttga aaaaaatcca ataatgtata caatcacccc taaatataaa taatgggtgt
     1861 atatgatcag acgatttggc attgtagcga tgtgtgcaat gggcgtgctt ggcgtcgggt
     1921 atgccgaccc ggtcgcttcc gtcgtggcga accacgcggt catgcccgtt cccttcgtgt
     1981 cgtcaattcc ctccacgcac accacacccg acctctcgct tcggaataaa atcgtcgaga
     2041 cggcgaaaaa gtacatcggc gtgcgatata atttcggcgg gatgagcgag aaggggttcg
     2101 attgctccgg gttcgtgaac cgcgtgtatc gcgaaaacgg catcgcgctg ccgcgaacgt
     2161 cgaaggcgca gttcagcaac ggcaaaaaag tctcgctcaa ggaggcgaag ccgggcgaca
     2221 tcgtgtattt cgtgcgctat ccccgacggc gcgcgacgca cgtgggcatc tatctgggcg
     2281 attcccagtt tatccacgcg cccagccggg gcaagcgggt ctcgatcagc aacatcggcc
     2341 agcgctactg gcgccaccgg cttattggcg tggtctcata tcttcccaaa aactccgaat
     2401 aggcgctccc tcacttgggc gccatccgga cggcgccgtc gatccgtatc gtttcgccgt
     2461 tgatgaacgc attttcgatg atgtgaacca ccagtcccgc aaattcctcc gggcgcccga
     2521 agcggctggg aaacggcacc tggctgatga gcgactgctt gagcgaatcc ggcatgagcg
     2581 tcgccatcgg cgtctcgaag atgcccggcg cgatggtgtt gttgcggatg ccgtatgcgg
     2641 ccagatcgcg cgcgatcggc agggtcattc ccacgatgcc cgccttggag gcggaatagg
     2701 cggcctggcc cacctgcccg tcgaaggccg cgaccgacgc cgtattgatg atcacgcccc
     2761 gctcgccgtc gggcgtgggc gcgtttttcg ccatctgcgc cgcggccagg cggatcacat
     2821 tgaaggtgcc gatgaggttc acgcgcagcg tgagctcgaa gagatcgagc gggaagggcc
     2881 ccttgccgct cacggtcttg gcggcggtgg cgatccccgc gcagttcacg acggcgttca
     2941 cggaaccgaa cgccgagacg gccgcctgga tcgccgcgct tacgtcgttt tcgctggcca
     3001 cgtccgtttt atggaagatc gccgcattgc cgagctcctt cgcgagcgcg gaccccttgt
     3061 cgtcggccat gtcgaggatg gccgcgcgtc ccccgccctt cacgatcgag cggaccgccg
     3121 cctcccccag gcccgacgcc ccgccggtca ccagcgcatg tacggtttca catttcatgg
     3181 gtcacctatt tcgctttttt ggatttgcac tcgtttcggc actgggcgaa ggtgctgttg
     3241 cacgtcttcg tcttgtaccg catccggccc acctggcccg gcgtcttctt cttgatgtcc
     3301 tgtacctcgt ccatgcaggc tttcttgtcc gtatagcatt ttttgatgca ctcggtttcg
     3361 gcgtcggcgg gcgcggcggc gtcatcggcc cggagcgcgg gcgaaaacag gacgagcgcc
     3421 gtcagggaca ggattgcgat ggcggttcga ttcatggtga cctcctcgat gatacggtgt
     3481 ggcatcgcgg aacaggcgat tgtcccatga tggtgcacga tatcgcgtgc agtgcaagaa
     3541 ttatattgcc cggcgggaaa aaaatcggtc cgatccgcgt gcgcgcgcat acagaaaggg
     3601 accgccgttc acggcggtcc ctttgaacga gagctcgttc aggtaatcgt gtcgcttact
     3661 tgccttctgc cggctgatcg acgggcttgt cggcaggctt gtccgcgggc ttgtcggcgg
     3721 cgggcttcac gcacgccttc ttgcagtcct tcgcggcttt cttgcactct ttcgccttcg
     3781 ccctcttttc cttgcccttc agcttcttca cttcggcgtt gcaggccttc atgtcggcgg
     3841 cgcacttctt gatgcactct ttgtcgacgg gcgtgccttt cttatgcttc ttcgcgtcgg
     3901 cgacattgat gaacatggcc gcaacagcga cagtcacgag aacgaccaaa aacttcttca
     3961 tacggctgac tcctttgtgg ttaaagtgtt ctatacgcat cgcccgttgc cgggtggacg
     4021 tattcatgta acaatccggg ccatggaagc ggccacggtt ttttcatttt ttacccattc
     4081 acggttccgg tcgaggcatc cgccggttcg cgctgcccgt cctggcggtg aatggcgtcg
     4141 ttgatgttaa aatcatcgag atcgtcatcc tcggcgtcgc cgcggtcgat cgacttggtg
     4201 atggtgtact tcttgtttgg ctcggccgtg acccgctcat cggaactgag cgacttgata
     4261 cgcatcgaac cgctcttcat gatcaccacc gtccggtcgc cggcgctctg gaggatgaac
     4321 tcgccctcga gcggtcgaag gtacgcttcc ggcgtcgaat agctgtattc gaggccgagg
     4381 gtgtttttcc tcatttgcga cagtatggtt cccctgataa gcctgatggt gtaggcccgc
     4441 cgtccgcgca cgcccggcgt tttcgcccgc tcgaacacca gaacgctgtc gtcgatcgcc
     4501 ttgataatgt aggcggaggt attggaaaac tcgattagcg aatccttgcc gacccggatg
     4561 cggctgttgg gcttgatctt cgaggtgagg tccgcctcgg cattgttgat gtacacgtcg
     4621 ccctcgatgt acgatatgtc catggtgacc tgtccgccgt tctcgccctg ccgaaccagg
     4681 agcgtcgttg tcgcaagcag gatgagcgcg gccgcgaggg ccgatgcgat ggcgagcgag
     4741 cggcgctcgc gtgcaaagtc gcgcacccgg tccaaacggc gtggagcgcc cgcgcgccgt
     4801 cggtacgatg cgcgtacgcg gcgaaggacc gcgtcggacg gcgccagcgc ggcattgtct
     4861 gatgcggcaa ggagcgtttc ggcggccgca aggcgcgcgt ggcaccgggg gcagtccgcg
     4921 gcatgcgtac gcatcgcgcg cgaccgcgcg gcgggaatcg ccccgtcgaa caggtcaagc
     4981 agctccgcat cgcgtatatg cgccgtctgt ttcatgccgt accggttatt tcccttccgt
     5041 tatcgccatg catgtgtaac aatcgtcgcg caatatgcgg ccacgaaaaa cggaagaaaa
     5101 acgaaaaaag ctaaaaaacc agctgcgtgg agcgcgtccc gcattcggtc gagcgtctcg
     5161 ctcaatcgtc gcctgacggt ccgttccgag tgtccggtcg cccgtgcgat ctccgggtag
     5221 gtgagtccca gctccttttt catgaggaag atggaccggt cgggctcgtc caatccctcc
     5281 accagggtgg cgatgagcgc ggcaagctcg cgcgattcgc tcagggcgtc cggcgccgca
     5341 gtgcgccggt ccggcgcgga ctcgtccagc ggccgggggc cgcgccggac gcggctccgg
     5401 tggcggttca cgcagaggtt ccgggccgtg gtgaacagga acgcgcgcag gttgtcgtcg
     5461 gtgatgggat agcgggcggc ataggacatc aggttctcga agcagtcgtg cagaatgtcc
     5521 tcggcggcct catgcgagcc cgtgtagcgg acgatgaaca caaagagctc ccggctgtgt
     5581 cgccgataga gggctatgag tttttcttca tcgagcacgg tgtcgttcgg tatgggtcct
     5641 gcgcgcggcc tgattggata cggctatacc ggggtatgtc cggtgtcaat tcgattgcgg
     5701 gacgcactaa actgaaaaag ggccgcgatc cgcgacatgc gcgaacgacg gccccttttc
     5761 gtgcgacgtg tggcgaagat cgccgcttac tt
//
LOCUS       DAORHF010000236         5786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14080_length_5786_cov_4.789391, whole genome shotgun sequence.
ACCESSION   DAORHF010000236 DAORHF010000000
VERSION     DAORHF010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14080_length_5786_cov_4.789391"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="PKJ16_15365"
     CDS             complement(<1..440)
                     /locus_tag="PKJ16_15365"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HNV48421.1"
                     /translation="MAINRVIALITDFGAGDFFVGAVKGAIKSIHPAAEIIDVSHGVA
                     PYSVLNAQFVLRSVHGYFPAGTVFMVVVDPGVGTGRRGLVARDGSYWYVLPDNGILSA
                     VASDELEVFSIRMEQYPDAAATFHGRDVFGPVAANIARGHAPAL"
     gene            649..1047
                     /locus_tag="PKJ16_15370"
     CDS             649..1047
                     /locus_tag="PKJ16_15370"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HNV48422.1"
                     /translation="MEASTEKTIHFRLEKSQEKLVQSDLMLQKGLYNDALLYSYLSMF
                     YAIRVLLINHETDSDDEERIMKLAEQYFEPAGWESIDVIAVLKESKKFKDSIEKTPGI
                     KIGREEADRLYRNAEQILAEVKRTVASPAR"
     gene            complement(1031..1465)
                     /locus_tag="PKJ16_15375"
     CDS             complement(1031..1465)
                     /locus_tag="PKJ16_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HNV48423.1"
                     /translation="MSERKPRIHIDIQDGPIDIAALCERAGTDADGAALLFVGRARNH
                     SNGKAVTHLDYEAYRSMAVRELEKIAQDACGRWDVGACAIVHRVGRVVIGDASICIAV
                     SSPHRDDAYRASRFIIDTIKKTVPIWKKEYYEDGSMWISERG"
     gene            complement(1467..1712)
                     /locus_tag="PKJ16_15380"
     CDS             complement(1467..1712)
                     /locus_tag="PKJ16_15380"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HNV48424.1"
                     /translation="MNVSIKTFASVRDALGYDERTVSVRDGSSVAELLREVLGGAPGL
                     MERAESLLVAVNEEYAAGDRIVRENDVVAIFPMVSGG"
     gene            complement(1709..2098)
                     /locus_tag="PKJ16_15385"
     CDS             complement(1709..2098)
                     /locus_tag="PKJ16_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48425.1"
                     /translation="MAPAKPVVVDPTRLIANINSASSSVALKAIGTVASGALKDPADV
                     MRARISLLRAVRNRRRPTPVRIAAIEAIAALNDRASVPVLVDQLEHETTPAVKRALEN
                     ALQVLSTIPGPNTPVEKPTRRGTEKIK"
     gene            complement(2246..2539)
                     /locus_tag="PKJ16_15390"
     CDS             complement(2246..2539)
                     /locus_tag="PKJ16_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48426.1"
                     /translation="MTSRGKSTEKAKRSSKKKTNLLRLYSEMNKYVIDAVDREVVRRL
                     KILADASEEDITKSSLTAVLRDPGGIELSAFSEGLQPYVKHYVFMVKRGKRKA"
     gene            complement(2625..3437)
                     /locus_tag="PKJ16_15395"
     CDS             complement(2625..3437)
                     /locus_tag="PKJ16_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV48427.1"
                     /translation="MTRLAPRTASRLRWAYAAIIAGVILASFVPQLLSGHSPFSMDDA
                     GACAPGSRHLLGADYLGRDVMSCTLAAGRISVVIGVGARLGSVIIGLAAGLLIGMSGG
                     AARSLLNGTVEVFLAIPALLLAMALAAVLGEGVWAVMTAIVAGTWAPVARFTAARVGD
                     ILSLDYVTAARALGAGRVRIMTRHLVPALLPSLVPLITTGIATSIMMEATLTFLGLGG
                     SASVTAIPTWGQLIQEGSKFLFDAPWIIVPPSVVLMIVILCFNQIGDSIAAD"
     gene            complement(3434..4342)
                     /locus_tag="PKJ16_15400"
     CDS             complement(3434..4342)
                     /locus_tag="PKJ16_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV48428.1"
                     /translation="MARRIAALVLVFLGAVLVVHTLVYLIPGDPAIVIAGEYADARDI
                     ERIRQELRLDDPFLVRYAVYVKRLVQLDLGNSIYSGAPVADIILQRFPATLMLALVSM
                     AIATLVGVAAGMGAAYYRDSIFDRAALWLSSLCVSTPIFVTCFILSLVFSYWLRLLPP
                     SGKEGLDPSYLILPALALASRSIALILRVVRNELIGVLRQHYIRTARAFGFSELRVVA
                     AFAFRNIITPVAVIVLLDFGAYLGGAVVTESVFAWPGVGRALVTALAKRDIPVMQGII
                     LFGTVLFLAIGVVIDLLQRATMRGKS"
     gene            4526..4657
                     /locus_tag="PKJ16_15405"
     CDS             4526..4657
                     /locus_tag="PKJ16_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48429.1"
                     /translation="MKCSRCGTEIHKLNHYYIPSGESSQGLRYCIKCAKEEKIVTLI"
     gene            complement(4688..>5786)
                     /locus_tag="PKJ16_15410"
     CDS             complement(4688..>5786)
                     /locus_tag="PKJ16_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV48430.1"
                     /translation="KAAGGIIISASHNPIEWNAFKLVNKTGTFLNAEEIASFFERMER
                     AGAYRRWDSVGSITCRDDAGDIHMARVREVINERIIKRKKFSIALDSVNGAGSIITQT
                     FLKKLGCAVHPVHCTITGTFPRGAEPLPENLKDLSRAVKRTKAHIGFAQDPDADRLAI
                     VDERGRPIGEENTIALVVEHLLARKKGRVVVNLSTTKAVDDVAARYGAPVVRTRVGEI
                     NVVDEMRRGGARIGGEGNGGVISPEVHLGRDSLVGIGYILEMMAERGKTISELVADLP
                     RYTMIKGKVEFRPGAIGASFFDQLAERYRGERITDIDGVRIDFERDEAFRGGWVHLRA
                     SNTEPIFRIIAEGRTKAQAEGLYAHFAAMVE"
BASE COUNT         1206 a         1861 c         1722 g          997 t
ORIGIN      
        1 aagagggccg gcgcgtggcc gcgggcgata tttgccgcca ccgggccgaa cacgtcccgt
       61 ccatggaagg tcgccgccgc atcgggatac tgctccatgc gaatggagaa gacctcgagc
      121 tcgtccgaag cgacggcgga gagaatgccg ttgtccggaa gcacatacca gtacgatccg
      181 tcccgcgcca ccaggccccg gcggccggtg cccaccccgg gatccaccac gaccatgaac
      241 acggtccccg ccgggaagta cccatgaacg gaacgcagga caaattgcgc attgagcacc
      301 gagtacgggg ccaccccatg ggagacgtcg atgatctcgg cggcgggatg aatggatttt
      361 atcgcgccct tgaccgcacc gacaaaaaaa tcaccggccc cgaagtccgt tatgagcgcg
      421 attacgcgat tgatggccac cctcccccgt tcgatgcggc gccctcgaag cgatatatat
      481 cacgcgcaac cgaccgaatt atgtcaatag cttttgctga agcgccgccg agcccgccgg
      541 caaaataacg agcatgacga aaaaaatgct tgatgatatc ggtctgaatg ggtatgttat
      601 gtacggttcg gagatcactg attgcggggt gcgccggacg gaactcctat ggaagcctca
      661 acagaaaaaa ccatccattt ccgcctggaa aaaagccagg agaagctcgt gcaatccgac
      721 ctcatgctgc aaaagggcct ctacaacgac gccctgctct attcatacct ttcgatgttt
      781 tacgcgattc gcgttctgct catcaatcac gaaaccgatt cggacgacga ggagcgcatc
      841 atgaagctgg cagagcaata cttcgagccg gccgggtggg aatcgattga cgtaatcgcg
      901 gtgctcaagg aatcgaagaa attcaaggac tccatagaga aaaccccggg aatcaagatc
      961 ggccgcgagg aggccgatcg gctgtaccga aacgccgagc agatcctggc ggaagtgaag
     1021 cgaacggtcg ctagccccgc tcgctaatcc acatcgagcc gtcttcgtag tactcttttt
     1081 tccagatggg aaccgtcttt tttatggtgt cgatgatgaa gcgcgacgcc cggtacgcat
     1141 cgtcgcggtg cggcgaggag accgcgatgc agatgctcgc atcgccgatg acgacccgcc
     1201 ccacgcgatg gacgatggcg caggcgccca cgtcccatct gccgcacgca tcctgcgcaa
     1261 tcttctcgag ctcgcgcacg gccatgctgc gatacgcctc atagtccagg tgcgtgaccg
     1321 ccttgccgtt cgaatgattg cgcgcgcggc ccacgaacag gagcgcggcg ccgtcggcgt
     1381 ccgtgcccgc acgttcacac aacgcggcga tatcgatcgg cccgtcctga atgtcgatgt
     1441 gaatgcgggg ttttcgttcg gacatcctag cccccgctca ccatcgggaa tatcgccacg
     1501 acgtcgtttt cccgcacgat ccggtcaccc gcggcatact cctcgttcac cgcgaccagg
     1561 agcgattccg cgcgctccat gagtccgggg gcgccgccaa gcacctcgcg gagaagctcc
     1621 gcgacgctgc ttccgtcgcg aacagatacg gtgcgctcgt catatccgag cgcatcacgc
     1681 accgacgcga atgtctttat cgaaacgttc atttgatctt ctccgtaccg cgcctcgtcg
     1741 gtttctcgac cggggtattc gggcccggaa tcgtcgaaag aacctgaagc gcgttttcga
     1801 gcgctcgctt cacggcggga gtcgtttcat gttccaactg gtcgaccagg accgggaccg
     1861 atgcccggtc gttgagcgcc gcaatcgcct cgatggccgc gatgcgcacg ggcgtcgggc
     1921 gccgacgatt gcgcaccgcg cgcaagagcg agatgcgcgc gcgcatgaca tcggccggat
     1981 ctttgagcgc gccggatgcg acggtcccga tggctttaag cgcgaccgac gatgatgcgc
     2041 tattgatgtt tgcaatgagc cgcgtcggat cgacgacaac cggcttggcg ggcgccacgg
     2101 cgcccgcgcg gggcgcatga cccgcatcgc tccgtgcgga cgctgatttc cgggtacacg
     2161 acaacggtat gaggataaaa aaacacggga acgaacagat gatggcgcga atcagcattg
     2221 ccgacgtctc gcggcgcacc ggccgctatg ccttccgttt tccgcgcttg accatgaaca
     2281 catagtgctt cacgtagggc tgcaatccct ccgagaaggc gctcagctcg atgcctccgg
     2341 ggtcgcgcaa cacggcggtg aggctcgact tcgtgatgtc ttcctcggac gcgtcggcga
     2401 gaatcttcag gcgtcgcact acttcgcgat cgaccgcgtc aatgacgtac ttgttcatct
     2461 ccgaatacag gcggagcagg ttcgtttttt tcttcgacga acgtttcgct ttttcggtcg
     2521 actttcctct ggaagtcatt cagccatctc catcgtgcaa tgatcggcga tggtagtgac
     2581 gccccctcct attgtaaaca tatttttttt tgagtgtgat ttttttaatc cgcggctatt
     2641 gaatcgccga tctggttgaa gcacaggatc acaatcatca acaccacgga cggcggcacg
     2701 atgatccacg gcgcgtcgaa aagaaacttc gagccctcct gtatgagctg cccccacgtc
     2761 ggtatcgcgg tgacggacgc gcttccgccg agcccgagaa acgtgagcgt cgcctccatc
     2821 atgatgctcg tcgcgatccc ggtggtgatc aggggaacca gcgacgggag caacgcggga
     2881 acgagatggc gcgtcatgat ccgcacgcgc cccgcgccga gcgcacgggc cgccgtcacg
     2941 tagtcgagcg agagaatgtc gcccacgcgc gcggcggtga atcgcgcaac gggcgcccag
     3001 gtgcctgcga cgatcgcggt catcaccgcc cacactccct cgccgagaac ggcggcgagc
     3061 gccatcgcga gcagaagcgc ggggatcgcc aggaacacct cgacggtccc gttgagaagc
     3121 gatcgcgccg cgccgccgct cattccgatc agcagtccgg ccgcaagacc gatgatgacg
     3181 ctccccagcc gggcgccgac gccgatgacc acggaaatcc tccccgcggc cagcgtgcag
     3241 ctcataacgt ctcggccgag ataatcggcg ccgagcagat gccgcgaccc cggcgcgcac
     3301 gcgccggcat catccatcga aaacggtgaa tgccccgaca gaagctgggg aacgaacgac
     3361 gcgagtatca caccggcgat gatcgccgca tacgcccatc gcagccggga cgctgtgcgc
     3421 ggggccagac gcgtcacgac ttcccccgca tggtcgcgcg ctgcagaaga tcgatcacca
     3481 cgccgatcgc gagaaagagg acggtcccga acaggatgat cccctgcatg acgggtatgt
     3541 cgcgcttggc gagcgccgtg accagcgccc gcccaactcc cggccacgcg aacaccgatt
     3601 cggtgacaac cgcacccccg agataggcgc cgaaatcgag cagcacgatt acggccaccg
     3661 gcgtgatgat gttacgaaac gcgaaagcgg cgaccacgcg cagctcggag aacccgaatg
     3721 cgcgcgcggt ccgaatgtag tgctgccgca gcaccccgat gagctcattg cgcacgaccc
     3781 gcaggatgag cgcgatggag cgcgaggcga gcgccagcgc cggcagtatg aggtacgagg
     3841 gatcgagccc ctccttgccc gagggcggga gcagccggag ccaataggag aacacaagcg
     3901 acaggatgaa gcaggtcacg aagatgggcg tggaaacgca cagggacgag agccacaggg
     3961 cagcccgatc gaagatcgag tcccggtaat aggccgcccc cataccggcc gcgacgccga
     4021 caagggtcgc gatggccatg gagacgagcg cgagcatcag cgtcgccggg aagcgctgca
     4081 ggatgatatc ggccaccggc gcgcccgaat agatggagtt cccgaggtcc aactgcacca
     4141 gacgcttcac atacaccgca taccggacca ggaaggggtc gtcgaggcgc agctcctgcc
     4201 tgatccgctc gatgtcgcgc gcatcggcgt attccccggc gatcacgatc gcgggatcgc
     4261 cggggatgag atagaccagg gtgtgcacca ccaggacggc gcccaggaac acgaggacga
     4321 gcgcggcgat gcgccgtgcc atcgccatca tccttacgcc gttattcgct gataactgat
     4381 tcgttttcac gggatgtacc gacatgcgac cacgagcgga ggccaccgga agaaaatcat
     4441 gccaacgcac acaatagagt aaaaaatacc gggcgttctt ccgatactat aaccaacgaa
     4501 atccgtatat acccaggagt gcgccatgaa atgctctcgt tgcggaaccg agatacacaa
     4561 gctcaaccac tactatatcc catcggggga aagcagccag ggactccggt attgcatcaa
     4621 gtgcgcgaaa gaagagaaaa ttgtaacgct gatatagccg tcgccgcggc gacatgcggc
     4681 gcgggcgcta ctcgaccatc gccgcgaaat gcgcgtacaa gccctccgcc tgcgccttgg
     4741 tgcgcccctc cgcgatgatc ctgaagatcg gctcggtgtt cgatgcgcgc aggtgcaccc
     4801 accccccgcg aaaggcctcg tcccgctcga aatcgatccg cacgccgtca atgtccgtaa
     4861 ttcgctcgcc ccggtaccgc tccgcgagct gatcgaaaaa cgaagcgcca atcgcacccg
     4921 gccgaaactc caccttgccc ttgatcatcg tgtagcgggg caggtccgcg accagttccg
     4981 atatcgtttt cccgcgctcg gccatcatct cgaggatata gccgataccg acgaggctgt
     5041 cccggcccag atgcacctcc ggggaaatga cgccgccgtt tccctcgccg ccgatccgcg
     5101 cgccgccccg acgcatctcg tccaccacgt tgatctcgcc cacgcgcgtc ctgacgaccg
     5161 gagcgccgta gcgcgcggcc acgtcatcga ccgccttggt cgtcgagaga ttgaccacca
     5221 cccgaccctt ctttcgcgcg aggaggtgct ccacgaccaa tgcgatggtg ttctcctccc
     5281 cgatgggacg gccgcgctcg tcgacgatcg cgagtcggtc ggcgtcgggg tcctgggcga
     5341 acccgatgtg cgccttcgtt cgcttcacgg cgcgggaaag gtccttcagg ttttcaggga
     5401 gcggctcggc tccgcgggga aaggtcccgg tgatcgtgca atggaccgga tgcaccgcgc
     5461 acccgagttt tttcagaaac gtttgcgtga tgatcgaccc cgcaccgttt acggaatcga
     5521 gcgcgatcga gaattttttc cgtttgatga ttcgctcgtt gatcacctca cgcacgcgcg
     5581 ccatgtggat atcgccggcg tcgtcacgac aggttatgga gcctactgag tcccagcgac
     5641 ggtatgcccc cgcccgctcc atgcgctcaa aaaaggaggc gatctcttca gcgttcaaga
     5701 aggtgcccgt tttattgacc agcttaaagg cgttccattc aattggattg tgcgacgcgg
     5761 atatgatgat ccccccggcc gccttg
//
LOCUS       DAORHF010000237         5772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14126_length_5772_cov_5.008046, whole genome shotgun sequence.
ACCESSION   DAORHF010000237 DAORHF010000000
VERSION     DAORHF010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5772
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14126_length_5772_cov_5.008046"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..159)
                     /locus_tag="PKJ16_15415"
     CDS             complement(4..159)
                     /locus_tag="PKJ16_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48431.1"
                     /translation="MHLVKRITVSIALGAFAALGCAAHGPLSPEDAFARLTTAYARSD
                     AAALRPL"
     gene            complement(147..1016)
                     /gene="panC"
                     /locus_tag="PKJ16_15420"
     CDS             complement(147..1016)
                     /gene="panC"
                     /locus_tag="PKJ16_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HNV48432.1"
                     /translation="MKIVKTIAELRSTLAGPRANGARIGFVPTMGYLHRGHISLVEIS
                     KQHSDYQVMSIFVNRIQFNDPKDFETYPVDLKRDFDMAEAAGVDAVFLPDDAEMYRAQ
                     RTFVDIELLTNHLCGAHRPGHFRGVYTVVCKLFNIVQPDVSVFGQKDIQQAITIERMV
                     ADLNIPVRIIIAPIVREPDGLAMSSRNKHLGAEVRERAVVISRSLRRAEELIAQGVRS
                     AGDITASMREIIESGGPSAIDYISMVRYDDLQPVDRLEDPSVIAVASFFGSTRLIDNM
                     IINKTNGGYACIW"
     gene            complement(1085..1933)
                     /locus_tag="PKJ16_15425"
     CDS             complement(1085..1933)
                     /locus_tag="PKJ16_15425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV48433.1"
                     /translation="MESVSCPICVDSSNIPIFTKPSGRGEPFTLVRCAWCGLRFLDPR
                     PDENEIVAYYEREYFTTRTDRGYDNYFSPPVRSEIERVIALNCADLHLDDLERAIPGE
                     RRSLDIGCAAGYFVNYMRDRGWRASGVDIARDCIDFAADRLSLDVAFGNYLDMSYPHR
                     FHLITLWATIEHLHRPLDVLRKAHDDLADDGRLYLTTCRDDRWTFGRLKGAAWRYYNF
                     PEHLFFFSRKTMIALLDRAGFAIERFLTYGSGFGAPSSPLRRIADSMAKRWAMGDMML
                     LSARKK"
     gene            complement(1937..3070)
                     /gene="lpxB"
                     /locus_tag="PKJ16_15430"
     CDS             complement(1937..3070)
                     /gene="lpxB"
                     /locus_tag="PKJ16_15430"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HNV48434.1"
                     /translation="MKRTIFIMTGEASGDLHGAMLVRHIREITDEYQFVGVGGSEMEQ
                     AGVTLIDHYSQYSAMGLIEPLKKIRFFQRAFKRITAYIADNAINTVITVDYPGFNLPL
                     AKRLYRHRPRVIHYISPQLWAWHYSRIHDIRKFFYAVVALYPFEVDMYRAEGVRAYFH
                     GNPLVDSTLAKLADGAPLTVPDSIEYVALLPGSRLSEVSRHIKPLVDAAVILRRERPV
                     EFLVPILSGPSGEFVRRYLADHCPDGLPVHIVENNTQRAIERARIVITSSGTATLETA
                     ILETPMIIIYKVSIPFEILGKLLVRVKDIGLVNIVAGRRICPELTQRDASGAGIAREA
                     MRLLADPAAYDTMLAGIRAVKERLGAPGAIRRIARQFVELIEE"
     gene            complement(3070..3876)
                     /gene="lpxI"
                     /locus_tag="PKJ16_15435"
     CDS             complement(3070..3876)
                     /gene="lpxI"
                     /locus_tag="PKJ16_15435"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF017989.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HNV48435.1"
                     /translation="MAEAERIGLIAGDGTLPVTLARRLEAQGLLAIVIVLQGNTERFG
                     LGPDLVIGMPPGMIKRILKKLKAYNVNRIIMLGTISKQGFIERKGFDLYALKAFKRLR
                     NDNDMTLFGIINETFEEWGMTVLPQDKYLADCIANTGVLTKKKPRGGMMDDAVFGMGL
                     ARQFASMDIGQSIVVKNGTVLAIETVEGTDETIRRGGALGKKGSVVCKAARKNQDPRF
                     DIPAVGAHTITTMAHSGCAMIALEAGRMFLVDRDETIRLADSHGIVIVGV"
     gene            complement(3876..>5772)
                     /gene="dnaE"
                     /locus_tag="PKJ16_15440"
     CDS             complement(3876..>5772)
                     /gene="dnaE"
                     /locus_tag="PKJ16_15440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV48436.1"
                     /translation="VEYVPLYKDAKDGSISTQFEKVSLEKAGLVKMDFLGLKNLSIID
                     ACVKRIAQTRGVAIDIGAIPLDDEPTFRLLQEANTLGVFQLESAGMQNILRKLKPTAL
                     DDIIAVNALYRPGPLDSGMVDDYIIRKRNPAKISYPHASLEPVLKDTLGVIVYQEQVM
                     LISQVIAGFSLPEADKLRKAMGKKDEAIMNALEEKFVKGAHQKGHDPSFSENLYSMIK
                     QFSRYAFNKSHSAAYAMVAYQTAYLKAHYTAEFMAALLSAQPDSQEDVIKYINDCKAN
                     GIAVLPPSVNSSEYDFTIENGSIRFGLSAIKGIGEKAIESIIAARKRCGTFATLEQFL
                     ENIDLFAVNKGVIESLAKAGALDALHPNRAQLLAATDHLLETAKRIQLDKSSGQGSLF
                     DFNEPDGPAKTFSMIELPQVRDWHDNEKLVHEKEVLGIYVSGHPLAKYENEIKALSCT
                     SIARLSEKKTSGDVSIIGIITNLKKRTSKNGNQFAVGVLEDMEGMIETIFFPNVYTKH
                     AELIESDSPVMVIGNIEYDDATPKKLMTREVKSLQQIQREAISAVHIRLDLVGVDDNL
                     LNDLKSIMERNKGECQVFLHLSRTNGDEQTIRVHHTFNISPSERLIKDITGIVGQDAI
                     FYSLGHC"
BASE COUNT         1048 a         1642 c         1865 g         1217 t
ORIGIN      
        1 ggcttacaac ggcctgagcg cggcggcgtc cgatcgcgca taggccgttg taagccgcgc
       61 gaaggcgtcc tcgggtgaaa gcggaccgtg cgcggcgcac cccagcgcgg cgaacgcgcc
      121 gagcgcgatg gagactgtga ttcgttttac cagatgcatg cgtacccgcc attcgtttta
      181 ttgataatca tgttgtcgat gaggcgcgtc gatccgaaaa aggacgcgac ggcgatcacg
      241 gagggatcct cgagacggtc caccggttgc agatcatcgt agcggaccat ggagatgtaa
      301 tcgatggctg acggaccgcc tgattcgatg atctcgcgca tggaggcggt gatgtcgccg
      361 gcgctgcgga cgccctgggc gatgagctcc tcggcgcgtc gcaaactgcg gctgatgacg
      421 accgcgcgtt ccctcacttc ggcgccgagg tgcttgttgc gcgagctcat cgcaagcccg
      481 tccggttcgc ggacaatggg cgcgatgatg atccggacgg gaatattcag gtcggcgacc
      541 atccgttcga tggtgatcgc ctgctgaatg tccttttggc cgaagaccga tacgtcaggt
      601 tgcacgatgt tgaacagctt gcagacgacg gtatagaccc cgcgaaaatg accgggccga
      661 tgcgcgccgc ataggtggtt cgtcaggagc tcgatgtcca cgaacgtgcg ttgggcgcgg
      721 tacatctcgg catcgtccgg gagaaagacc gcatcgaccc cggcggcctc ggccatgtcg
      781 aaatcacgtt tgaggtccac cggataggtt tcgaaatcct tcggatcgtt gaactggatc
      841 cggttcacga aaatactcat gacctgatag tcgctgtgct gtttcgatat ctcgaccagg
      901 ctgatgtgcc cccggtgaag atagcccatg gtggggacaa aaccgattcg cgccccattg
      961 gcgcgagggc cggccagtgt tgagcggagt tcggcgatgg tttttacaat cttcatggcg
     1021 tcgatatcct ccctgcggtg ccggttttat tcaaatggag cacgatgatt gtcaaacgca
     1081 attttcactt cttgcgtgcc gaaagcagca tcatgtctcc catcgcccac cgcttggcca
     1141 ttgagtcggc gatcctccgt aacggtgaac tgggcgcgcc aaaaccgctt ccatacgtca
     1201 ggaagcgctc gatggcgaac ccggcgcggt cgaggagcgc gatcatcgtt tttcgtgaaa
     1261 agaaaaacag gtgctcgggg aagttgtagt agcgccacgc ggcgcccttg aggcggccga
     1321 aggtccagcg gtcgtcccgg caggtggtga gatagagtcg gccgtcatcg gcaagatcgt
     1381 cgtgcgcttt gcgcaacaca tcgagcggtc gatggaggtg ctcgatcgtg gcccagagcg
     1441 tgatcagatg aaaacgatgc ggatatgaca tgtcgagata gttgccgaag gcgacatcga
     1501 gggacaggcg gtcggctgcg aaatcgatgc agtcgcgggc gatatcgacg ccggacgcgc
     1561 gccagccgcg atcgcgcatg tagttcacga agtatcccgc ggcgcagccg atatcgagcg
     1621 agcgtcgttc tccggggatg gcgcgttcga gatcgtctaa atgtagatcg gcgcaattga
     1681 gcgcgatcac ccgttcgatc tcgctgcgca ccggcggcga gaagtaatta tcgtagccgc
     1741 ggtcggtgcg ggtggtgaaa tactcgcgtt cgtagtaggc gacaatttca ttctcgtcag
     1801 ggcgcggatc caggaagcgg agcccgcacc aggcgcaccg gacgagggtg aagggctcgc
     1861 cgcgaccgga cggtttggtg aagatcggga tgttcgacga atcgacgcag atggggcagg
     1921 ataccgattc catccgctat tcctcgatga gctcgacgaa ctggcgtgcg atccgcctga
     1981 tggcgcccgg cgcgccgagc cgctccttaa ccgcgcggat accggccagc atggtatcgt
     2041 aggcggccgg atccgcgagg aggcgcatcg cctcacgcgc aattcccgca ccggacgcgt
     2101 cccgctgggt gagctcgggg cagatgcggc gcccggcgac gatgttcacc aggccgatgt
     2161 ctttgacgcg caccaggagc tttccgagga tttcgaacgg aatgctcact ttgtagatga
     2221 tgatcatcgg cgtttcgagg atcgccgtct cgagcgtcgc ggtcccggag gaggtgatca
     2281 caatgcgtgc tcgctcgatc gcgcgctggg tgttgttctc gacgatatgg accgggagtc
     2341 cgtccgggca atggtcggca agataccggc ggacgaattc gccggagggg ccggagagga
     2401 tcggcaccaa aaactcgacc ggacgctcac ggcgcagtat caccgccgca tcgacgagcg
     2461 gcttgatgtg gcgggaaacc tccgacagtc gcgaaccggg gagaagggcg acatactcga
     2521 tcgaatcggg taccgtgagc ggcgcgccgt ccgcgagctt ggcgagggtt gagtcgacga
     2581 gcggattgcc gtgaaaatag gcgcgcacgc cctcggcccg gtacatgtcg acctcgaagg
     2641 ggtacagcgc caccaccgcg tagaagaact ttcgtatgtc gtgtatgcgc gaatagtgcc
     2701 aggcccacag ctgcggcgag atatagtgaa tgacgcgcgg gcgatgacgg tagagccgct
     2761 tggcgagggg gagattgaag ccgggataat cgaccgtaat caccgtgttg atggcgttgt
     2821 cggcgatata ggcggtgatg cgcttgaatg cgcgctggaa gaagcggatc ttcttgagcg
     2881 gctcgatgag tcccatcgcg gagtactgtg aatagtggtc gatgagcgtg acgccggcct
     2941 gttccatctc cgagccgccg acgcccacga attgatactc gtcggtgatc tccctgatgt
     3001 gccggacgag catggcgcca tggagatcgc cgctcgcctc gccggtcatg atgaaaatcg
     3061 tccgcttcat cagacgccca cgatcacgat gccgtgcgaa tccgcgaggc gtatggtctc
     3121 gtcgcggtcg accagaaaca tcctgcctgc ctcgagcgcg atcatggcgc acccgctgtg
     3181 ggccatcgtg gtaatggtgt gtgcgccgac cgcgggaatg tcgaagcgcg gatcctggtt
     3241 cttgcgcgcc gccttgcaca ccacggaccc ctttttgccg agggcgccgc cgcgccggat
     3301 ggtctcgtcg gtgccctcga cggtctcgat ggcgagcacc gtcccgttct tcacgacgat
     3361 ggactgaccg atgtccatcg acgcgaactg ccgcgcaagt cccatgccga agacggcgtc
     3421 gtccatcatg ccgccccggg gcttcttttt cgtgaggacc cccgtgttcg cgatgcagtc
     3481 cgccaggtat ttgtcctgcg ggagcaccgt catgccccat tcctcgaagg tctcgttgat
     3541 gatcccgaag agggtcatgt cgttgtcgtt tctgaggcgc ttgaaggcct tgagcgcata
     3601 caggtcgaac cccttgcgtt ctatgaagcc ctgtttgctg atcgtgccga gcatgatgat
     3661 gcggtttacg ttgtatgcct tcagcttttt cagaatgcgc ttgatcatcc ccggcggcat
     3721 gccgatcacg aggtcggggc cgagcccgaa ccgttcggtg ttcccctgca ggacgatgac
     3781 gatcgcgagc agcccctgcg cttcgagacg gcgagcgagg gtgacgggca gggtgccgtc
     3841 cccggcgatg agcccgatgc gttccgcctc cgccatcagc aatgccccag cgaatagaag
     3901 atcgcgtcct gtcccacgat gcccgtgatg tccttgatga gccgctcgga cggtgaaata
     3961 ttaaaggtgt gatggacgcg gatggtctgc tcgtcgccat tggtgcggct taaatgcagg
     4021 aacacctggc actcgccctt gttgcgctcc atgatcgact tgaggtcgtt gagaaggttg
     4081 tcgtcgacgc ccaccaggtc gagacggatg tggactgcgg agatggcctc gcgctggatc
     4141 tgctgcagcg atttcacctc gcgggtcatg agcttcttcg gcgtggcgtc gtcgtattcg
     4201 atattgccga tcaccatcac cggcgaatcg gactcgatga gttcggcgtg tttggtgtag
     4261 acgttcggga aaaagatggt ctcgatcatc ccttccatgt cctcgaggac gcccacggcg
     4321 aactggttgc cgtttttgga cgtgcgtttt tttaaattcg tgatgatccc gatgatggac
     4381 acgtctcccg atgtcttttt ttcgctcagc cgagcgatcg acgtgcacga gagcgccttt
     4441 atctcgtttt catattttgc aagcggatgg cccgagacgt agatccccag cacctccttt
     4501 tcgtgtacga gcttttcgtt gtcgtgccag tcgcggacct gcgggagctc gatcatcgaa
     4561 aaggttttcg ccgggccgtc cggttcattg aaatcgaaca ggctcccctg gccggacgat
     4621 ttatcgagct gaatgcgctt ggctgtctcg agcaggtggt cggtggccgc gaggagctgg
     4681 gcgcggttcg gatggagcgc gtccagcgcg ccggccttgg cgagcgactc gatgacgccc
     4741 ttgttgacgg cgaacaggtc gatgttctcg aggaactgct ccagcgtcgc gaaggtgccg
     4801 catcgctttc gggcggcgat gatggactcg atcgcctttt cgccgatgcc ctttatggcg
     4861 ctgaggccga atcgtatcga gccgttctcg atggtgaaat cgtactcgct gctgttcacg
     4921 ctcggcggga gcacggcgat cccgttggcc ttgcagtcgt tgatatactt gatgacgtct
     4981 tcctggctgt ccggttgcgc ggagagcaaa gcggccatga actcggcggt ataatgcgcc
     5041 ttcaaatatg ccgtctggta ggcgaccatg gcatacgcgg ccgaatgcga cttgttgaag
     5101 gcgtatcggc tgaactgctt gatcatcgag tagaggtttt cgctaaagga cggatcgtgc
     5161 cccttctggt gcgcgccctt cacgaatttt tcctcgagcg cgttcatgat cgcttcgtct
     5221 tttttgccca tcgccttgcg gagcttgtcg gcctcgggga ggctgaaccc ggcgatcacc
     5281 tgggagatga gcatcacctg ctcctggtac acgatcacgc cgagcgtgtc cttgaggacg
     5341 ggctcgagcg acgcgtgcgg atagctgatc ttggcggggt tgcgtttgcg aatgatgtag
     5401 tcgtccacca tgcccgaatc gagcggaccc ggccggtaga gcgcgttgac cgcgatgatg
     5461 tcgtcgagcg ccgtgggctt cagctttcga aggatgttct gcatgcccgc gctttccaac
     5521 tggaacacgc cgagcgtgtt cgcctcctgg agcaggcgga aggtgggctc gtcgtcgagc
     5581 ggaattgcgc cgatatcaat cgccacccca cgggtctgtg cgatccgttt cacgcatgcg
     5641 tcgatgatgc tcaggttctt gagccccagg aagtccatct tgaccagccc ggccttttcg
     5701 aggctcacct tttcgaattg cgtggagatg ctgccgtcct tggcgtcctt gtacaggggg
     5761 acgtattcga ca
//
LOCUS       DAORHF010000238         5756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14202_length_5756_cov_5.113138, whole genome shotgun sequence.
ACCESSION   DAORHF010000238 DAORHF010000000
VERSION     DAORHF010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14202_length_5756_cov_5.113138"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PKJ16_15445"
     CDS             complement(<1..405)
                     /locus_tag="PKJ16_15445"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HNV48437.1"
                     /translation="MFMRKIDQYIIRQFLFALSGSITFMVGVFLITNVIDQLKYFSDP
                     RVPGFTVFLYLINYIPEVLTQILPAAALFSTSFMVGYLNQSNEIVAIYNGGIAFTRLL
                     IPLLLVGVLIALGSFFFSEFVAADASKRSFELF"
     gene            complement(450..1334)
                     /locus_tag="PKJ16_15450"
     CDS             complement(450..1334)
                     /locus_tag="PKJ16_15450"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV48438.1"
                     /translation="MTTTARAEGIVDLHAHTTASDGIYAPARLVDLAIESGLRAIAIT
                     DHDTIDGLDEGLARAAERGFLCVPGVELSVEYEGGTFHLVGLSVDRRNRALCDTLEEL
                     KLIRSKRVDGIINDLNRHGIAITAEELHGVSGDTAIGRPHIARALIRKGYGTSMTEIF
                     SKYLVRGKPGYVKKRKIAPSEAVRLITDAGGIPVIAHPISLNFKLFARFEEILDGLVA
                     IGVRGVEAYASMHTPEEAERFAEIARSRGLLVTGGSDFHGDKNERLGFYADGSPIPAE
                     ILADVENFGCAEPRGLVS"
     gene            complement(1409..2407)
                     /locus_tag="PKJ16_15455"
     CDS             complement(1409..2407)
                     /locus_tag="PKJ16_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HNV48439.1"
                     /translation="MRGYYKKIKGKQYDGHLIRLADESVKGKGDGRISLKDAKKILAA
                     VKDSDAYTDIEKATMHFIRDHYAFTGEANAWFRTEIRKWAATKGAAAGSAKKRVPAVK
                     KAAAATPVKKQKPKAAPKKTAKKAVKKHARPSAAPKKEPVYVTESFEERARFDQRVVE
                     HEEEKRSSRFVLALVLVAFLAIGALIVFYPKLQRLFTKSDDAPVRKDQPAQDASVRSV
                     TPSKDAPATDIPSTMVPAKDAAAPGAGAPPKFESYKAEGRENYDYYIVKPKETLVTIG
                     EQLTGKYWDWEKLFNENKRIIKDPRVVYAGQIIKIPDSMKKQIERRQSKTGAKSAE"
     gene            complement(2434..2506)
                     /locus_tag="PKJ16_15460"
     tRNA            complement(2434..2506)
                     /locus_tag="PKJ16_15460"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2471..2473),aa:Arg,seq:ccg)
     gene            2647..4605
                     /locus_tag="PKJ16_15465"
     CDS             2647..4605
                     /locus_tag="PKJ16_15465"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV48440.1"
                     /translation="MVTSSPARCILALTVLLAGVSAAHAALIVTDQDQRVMLGRHSEI
                     LEDPDGALGIEQVSGPAMEHRFERVTRDTVNRATVRGVFWLKVPIEYKPAPGGNRTPQ
                     WVLEIAYPSLETVTLFLPAGGGGWTVTQAGEALPYAVREILHRNPVFAVPLPRDRPVT
                     IFIRAAVEKVMVLPPVLWQREAFVRAAYTEQLIHGLYFGIILVMVLYNLFVFISLRDP
                     SYAFYVLYTASFGLFQFSFTGLAFEYLWPEAPAWNSMANVVLVFVSFSFAMQFSRHYL
                     LMGRHWPLLARIMDGLTAVGVLCAGLTVFVDRTMMTHASNLLPACGVVVFIAVGVLAL
                     RRKSRAARYFLVAWGAYIVGVTLFILKNLGVLPANIITMYGIQAGSAIEIILFSLGLA
                     DRINELRREREQSQQRLLTIQHEMDVAQRIQNSLLPGTIPRLAGAAITVRYAPARCIG
                     GDYYDFAQTGPSSCGVLIADTSGHGIPAAIISSALRLSFHEQRKITHRPPALIRGMNE
                     MLIGNIGNQYITAGYVFVDLAAMTAQYVNAGHQPLCVVDRADGAIRQVSPKGIILGWR
                     QFDDFELCEFPLAQNQRLIFITDGYVECRNGAGELFEHDRLVEAFRELRALPPDECID
                     ALDERISAWNTGRDEFEDDRTILIIDIE"
     gene            complement(4684..5064)
                     /locus_tag="PKJ16_15470"
     CDS             complement(4684..5064)
                     /locus_tag="PKJ16_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV48441.1"
                     /translation="MIAQLTLLDRLRVPTYDDGSQMRYGALVIDRSRCRECGICVRIC
                     PGACLVTDTATKMSLLNGTTHKGKSGVPRLDLMKSGEAGCIACFDCGTACPHGAISLA
                     GHFNPGYRFKRIAQEDVLAYPQRY"
     gene            complement(5066..>5756)
                     /locus_tag="PKJ16_15475"
     CDS             complement(5066..>5756)
                     /locus_tag="PKJ16_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV48442.1"
                     /translation="VERVILERRSIRSFRPEPLPDGMIRRILEAGRFAPSAGNMQPWR
                     FVVIKSPEIIAAMERDAVRIARLFMFFLDYARGGRVRRAITMPLAKLFIRLLPNELHA
                     PPFMLLSQIAQGRAPVFHNAPVIVLILEDRRGVSCPAVDIGVCGQNMVLAAHSMGAGS
                     CWIGLIKLLRYYPKWRRRLGARYPYHMNESIAFGWPRPTADGLVPREVQIVEWREGGP
                     DDPPRYERQGG"
BASE COUNT         1013 a         1843 c         1759 g         1141 t
ORIGIN      
        1 aaacaactcg aaggagcgct tgctggcgtc ggcagcgacg aactcggaga agaaaaacga
       61 cccgagcgcg atcaacacgc ccaccaggag cagggggatg aggagccgtg tgaaggcgat
      121 gcccccgttg tagatggcca cgatctcgtt ggactggttc aggtatccca ccatgaacga
      181 cgtcgagaac agggccgccg cgggaaggat ctgcgtgagc acctcgggga tgtaattgat
      241 cagatagaga aatacggtaa agcccggcac gcgcggatcg gagaaatact tgagctgatc
      301 gatcacgttc gtgatgagga acaccccgac catgaaggtg atggagcccg aaagcgcgaa
      361 gaggaattgc cgtatgatgt attggtcgat tttgcgcatg aacatgatag acgtcccggg
      421 gcgcgtaccg gtgcgccgcc ttcagaacat cacgatacga ggccccgcgg ttcggcacaa
      481 ccaaaatttt ccacgtccgc caggatttcc gcgggaatcg gactgccgtc ggcatagaag
      541 ccgagccgct cgtttttgtc accgtggaag tcgctcccgc cggtcaccag gagaccgcgc
      601 gaccgggcga tttcggcgaa tcgctcggcc tcctcgggag tgtgcatcga ggcataggcc
      661 tccacgcccc ggaccccgat cgcgaccagc ccgtccagga tctcctcgaa ccgggcgaat
      721 agcttgaaat tgagcgagat cggatgggcg atcaccggaa tcccgccggc gtcggtgatg
      781 aggcgtacgg cctctgaggg cgcgattttc cgttttttga catagccggg cttgccccgc
      841 acaagatact tgctgaatat ctcggtcatg ctggtcccgt agcccttccg gatgagcgcc
      901 cgcgcgatgt ggggccgtcc gatggcggtg tcgcccgaga cgccatgcaa ttcctcggcc
      961 gtgatcgcga taccgtgacg gttgagatcg ttgatgatgc cgtcgacccg cttgctccgg
     1021 atgagcttga gctcctcgag cgtatcgcac agcgcccggt tgcgccggtc gacagacaga
     1081 cccaccagat gaaacgtgcc accctcgtat tcgacgctca attccacgcc ggggacgcag
     1141 aggaagccgc gttccgccgc gcgggccaat ccctcgtcca gcccgtcaat cgtatcgtgg
     1201 tcggtgatgg cgatcgcccg caggcctgat tcgatcgcca gatcgacaag gcgcgcgggt
     1261 gcgtagattc cgtcggaggc ggtggtatgc gcatggagat cgacgatccc ctctgcgcgc
     1321 gcggttgtgg tcatgtgccc tcagcaacgc cgccgtaacc cgtgcggatt acggcggcca
     1381 attcgtacac gatgtcgatc cggctgttct attcggccga tttcgcgccg gtcttcgact
     1441 gccggcgctc gatctgtttc ttcatgctgt ccgggatctt gatgatctgt ccggcataga
     1501 ccacccgggg gtccttgatg attcgcttgt tctcattgaa cagcttctcc cagtcccagt
     1561 acttgccggt cagctgctcg ccgatggtca cgagcgtctc cttcggcttg acgatataat
     1621 aatcgtagtt ttcgcgcccc tcggccttgt acgattcgaa cttgggcggt gcgcccgctc
     1681 cgggggccgc agcgtccttc gcgggcacca tggtcgacgg gatgtccgtt gccggagcat
     1741 ccttggacgg cgtcacggat cggaccgacg cgtcctgcgc gggttgatcc ttccgcaccg
     1801 gcgcgtcgtc gctcttcgtg aagagccgct ggagcttcgg atagaatacg atgagggcgc
     1861 cgatggcgag aaaggcgacc agcaccagcg ccaggacaaa acgcgacgag cgcttctcct
     1921 cctcatgctc gaccacgcgc tggtcgaaac gcgcgcgttc ctcgaagctt tccgttacat
     1981 acaccggctc cttcttcggc gccgcggacg gccgcgcgtg tttcttcacc gccttcttcg
     2041 cggtcttctt gggcgcggcc tttggttttt gctttttcac gggcgtggcc gccgcagctt
     2101 ttttcaccgc cggcacacgc ttcttggccg atcccgcggc cgcccctttc gtagccgccc
     2161 actttcgaat ctccgttcgg aaccacgcat tcgcttcgcc ggtaaaggcg taatggtcgc
     2221 gaataaagtg catggtggcc ttctcgatat cggtgtaggc gtccgagtcc ttgaccgcgg
     2281 cgaggatctt tttcgcgtcc ttcagtgaga tgcggccatc gcccttccct ttcaccgatt
     2341 cgtctgcgag cctgatgaga tggccgtcgt attgcttgcc ttttatcttt ttgtagtacc
     2401 ccctcatcgc ggtttcctcc attttttttg atgtgacccc gacaggagtt gaacctgtga
     2461 cgcccagatc cggaatctgg cgctctatcc tctgagctac ggggccattt acgcgtatgt
     2521 acggaacggg atatggagcg aaaagtcaat aacatttttc ccgagtgacg gtgtgaataa
     2581 gttatttgac aaaaaccata ctcgactatt tactcaaaga aaaacgtatg cgtccatgag
     2641 tggttaatgg taacatcgtc accggcccgc tgtattctcg cattgaccgt cctgctcgcg
     2701 ggcgtttccg ccgcgcatgc cgctctcatc gtcaccgacc aggaccagcg ggtgatgctg
     2761 ggacggcaca gcgagatcct cgaggacccg gacggcgcgc tggggatcga gcaggtgtcg
     2821 ggcccggcga tggagcaccg gttcgagcgc gttacccgcg acacggtcaa ccgcgccacg
     2881 gtgcgcggcg tcttctggct caaggtcccg atcgaataca aacccgcgcc gggcgggaat
     2941 cgcacccccc aatgggtcct cgagatcgcc tacccctcgc tcgaaacggt gacgctgttc
     3001 cttcccgccg gaggcggcgg ctggaccgtc actcaggctg gcgaggcgct gccgtacgcc
     3061 gtgcgtgaga tactccaccg caacccggta ttcgccgtgc cgctaccgcg cgaccgcccg
     3121 gtcacgatct tcattagggc tgcggtcgag aaggtgatgg tgcttccccc cgtgctgtgg
     3181 cagcgcgagg cgtttgtgcg cgcggcatac accgagcagc tcattcacgg actgtatttc
     3241 gggatcattc tcgtcatggt cctgtacaat ctcttcgtgt ttatttcgct gcgcgacccc
     3301 agctatgcct tctatgtgct gtacacggcc agcttcggcc tgttccagtt ctccttcacc
     3361 ggtctggcct tcgaatacct gtggccggaa gcgcccgcat ggaactccat ggccaatgtg
     3421 gtgctggtat tcgtctcatt ctcgttcgcg atgcagtttt cccggcacta cctgctgatg
     3481 ggccgccact ggcccctcct cgcgcgcatc atggacgggt tgaccgccgt gggcgtcctg
     3541 tgcgccgggc tcaccgtttt cgtggaccgg acgatgatga cgcatgcgag caatctcctg
     3601 cccgcctgcg gcgttgtcgt gtttatcgcc gttggcgtgt tggcgcttcg gcgaaagtcc
     3661 cgtgcggcgc ggtattttct tgtggcatgg ggcgcgtata tcgtcggcgt cacgctgttt
     3721 atattgaaga acctcggcgt gctgcccgcg aacatcatca cgatgtacgg catacaggcc
     3781 ggctcggcca tcgagatcat cctcttctcc ctggggctgg ccgaccggat caacgagctg
     3841 cgccgtgaac gggagcagtc gcagcaacgg ctcctcacca tccaacacga aatggacgtc
     3901 gcgcagcgca tccaaaactc cctgctcccc gggacgattc cccgcctcgc gggagcggcc
     3961 atcacggtcc gctacgcgcc cgcacgctgc atcgggggcg attactacga tttcgcacag
     4021 accggcccgt cctcctgcgg cgtgctgatc gcggacacct ccggccacgg gatccccgcg
     4081 gccatcatct cctcggcgct caggctgtcg tttcacgaac agcgaaagat cacgcaccgc
     4141 ccccctgcgc tgattcgtgg catgaacgag atgctgatcg gcaatatcgg caaccagtac
     4201 atcacggcgg gctatgtatt cgtggacctg gcggcgatga cggcgcagta cgtcaatgcc
     4261 gggcaccagc cgctgtgcgt cgtcgatcgc gccgacggcg ccatccgaca ggtatcgccc
     4321 aaggggatca tcctggggtg gcggcaattc gacgatttcg agctgtgcga atttcccctg
     4381 gcgcagaatc aacgcctcat ttttatcacc gacgggtatg tcgagtgccg caacggggcc
     4441 ggggagctct tcgagcacga tcgcctcgtc gaggcctttc gcgagctcag ggcgcttccg
     4501 cccgatgagt gcatcgacgc gctggacgag cggatctccg catggaacac cgggcgcgat
     4561 gagttcgagg acgatcgcac catcctcatc atcgacatcg aataggcgcg aagcgcccat
     4621 cgacacccct cccgcaccga ttgcgatacg gcgtcccgaa caccgcaaac gccaactggc
     4681 gaatcaatac cgctgaggat aggcgaggac atcctcctgg gcgattcgct tgaagcgata
     4741 gccgggattg aagtggcccg cgagcgagat cgccccgtgc gggcaggcgg tcccgcagtc
     4801 gaaacatgcg atgcacccgg cctcgcccga tttcatcagg tccaatcggg gaacgccgct
     4861 cttgcccttg tgcgtcgttc cgttcaagag cgacatcttg gtggcggtat cggtgaccag
     4921 gcaggcgccg gggcagatcc gcacgcagat cccgcattcg cggcaccgcg atcggtcgat
     4981 gacgagcgcg ccgtagcgca tctggcttcc gtcgtcgtag gtgggcacgc ggagccggtc
     5041 gagaagcgtc aattgcgcga tcatatcatc ccccctgccg ttcgtagcgc ggcggatcgt
     5101 ccggaccgcc ctcgcgccat tcgacgatct gcacctcgcg cggaacgagc ccgtcggccg
     5161 tgggccgggg ccatccgaac gcgatgctct cgttcatgtg atacggatat cgcgcgccca
     5221 ggcgacgccg ccacttcgga taatagcgga gcagcttgat gaggccgatc cagcagctcc
     5281 ccgcgcccat gctgtgcgcc gcaagcacca tgttctgccc gcacacgccg atatccaccg
     5341 ccgggcagga cacgcccctc cggtcctcga ggataaggac gatcacgggc gcgttgtgga
     5401 agaccggcgc gcgcccctgc gcgatctgcg agaggagcat gaagggcggc gcatggagct
     5461 cgttgggaag caatcgaatg aacagcttcg ccagcggcat ggtgatggcg cgccgcaccc
     5521 ggcccccgcg cgcgtaatcg agaaagaaca tgaagaggcg cgcgatccgc acggcgtcgc
     5581 gctccattgc ggcgatgatc tccgggctct tgatcacgac gaagcgccag ggctgcatat
     5641 tgcccgcgga gggggcgaag cgcccggcct cgaggatcct ccggatcatg ccgtctggga
     5701 gcggctccgg ccggaaggac cggatgctgc gccgctccag gatcacccgc tcgacg
//
LOCUS       DAORHF010000239         5708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14379_length_5708_cov_4.096409, whole genome shotgun sequence.
ACCESSION   DAORHF010000239 DAORHF010000000
VERSION     DAORHF010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14379_length_5708_cov_4.096409"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKJ16_15480"
     CDS             complement(<1..543)
                     /locus_tag="PKJ16_15480"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HNV48443.1"
                     /translation="MTFPTSMMIRPLLALLATLAFTTAATASAKERDPYIDQREIARA
                     KVERIEKRSASAERASLVELRIHLRVLEGSKKGKTHVAVYRGEDDMPKDMFYREGDTV
                     YIGISKFGGGEDVEQISIYDVDNTAGLIVLGALMFAAIVFVGRLRGALSILSLVATIV
                     LLFYVLIPLTLKGTPPLPIAV"
     gene            953..2452
                     /locus_tag="PKJ16_15485"
     CDS             953..2452
                     /locus_tag="PKJ16_15485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HNV48444.1"
                     /translation="MDITKNRLYAVVGAIVLFKLILAYAFPIWPDEGYYLTWTRNIDT
                     GFYCHPPMVGWLMYVMSFISRDIFFHRLIAIFVSTAAGFVIFAVARDITRDEKKAALA
                     SLVYFLSPANVFFIVLTNDAPLFLFTLLCVVCFYYAVARDRIIFSMLAGVFMGLAFLC
                     KYFAVLLFLSMVVYLAFNRNRRTLTHCLVAGIAALPIASINLYYNYMNCWNNVMVQWF
                     NRTGDKSFSALYFIGYVFGQLYWLTPWGLYYLVRERGGVAAFFAERTRLVFALALLAP
                     LAFMALIGLKAASLHWTLPFFSILPIVLLGLQSESLKRIVVGNAIFASAHALVIVVLL
                     LLPLSLFKDFRYYTDFVLYKKGDLICAELAPYRGKYVFGTIGYTVSSTLDYYCGEYFL
                     IFNSLSKWGRNDDKVTDFRKLDGKNILVVSPKIETADEYRHLFERVEVRYVDVLEGRF
                     SFIVGDGFKYAAYRDTTLRKALDKLYNVPTWLPCGSCYFFDRYFPERKY"
     gene            complement(2484..3275)
                     /locus_tag="PKJ16_15490"
     CDS             complement(2484..3275)
                     /locus_tag="PKJ16_15490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV48445.1"
                     /translation="MTYSIILPTYREKDNIVPLIARLIDVCAREGVSAEIIVVDDNSS
                     DGTDTAIAESFGHLGNAVRCYVRTHERGLASAIAYGINRANGEYLLVMDSDFNHDPER
                     VPVLIRHCAEHDLVAGSRFIDGGGMDNAAGRFWGSYAFNAIIRTALGMRTRDNLSGFF
                     VIRKSVLERIRHDSLFTGYGEYFIRILYYAHRLSLRIKEIPVVYLKRPSGTSNTRFVS
                     YSMTILKTIFVLRASKAPFLKQVFVETDAYRTMAMLSEMLSSVCI"
     gene            3621..4886
                     /locus_tag="PKJ16_15495"
     CDS             3621..4886
                     /locus_tag="PKJ16_15495"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014311932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HNV48446.1"
                     /translation="MSISKKTVRDIDFKGKRVIMRVDFNVPMKDGVVQDDTRIKAALP
                     TIQYVLEKGPKYLILMSHLGDPKKDTQKAKEKAEKDGKTFDEKKFMEGKHMMPPVAAY
                     LEKLLGKPVKIAPACFGPEVDAIVNGLGAGDVLMLGNTRMHKEETAKEAGDRETMAKA
                     MAAYADVYVNDAFGTAHRAHASTETMAKFLPAVAGFLMEKELEFLDDKIVKSPVKPFI
                     AVVGGAKVSSKIAVLESLLGKVSGIIIGGGMAYTFLKAMGKGIGKSLVEEDMLDTAKD
                     IMQKASAKGVKILLPLDHVEAKEFSAEAESKTTTSDGIDDGWMGMDIGPKTLAQFEQE
                     LAGAKTVFWNGPMGVFEFPKFAEGTTKIARLLAGLSATTVIGGGDSVSAVNKAGVADK
                     MSHISTGGGASLELVEGKVLPGVAALNDK"
     gene            complement(4942..>5708)
                     /locus_tag="PKJ16_15500"
     CDS             complement(4942..>5708)
                     /locus_tag="PKJ16_15500"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48447.1"
                     /translation="TACVQVGDDDDFIILDAGSGLRELGNHLMRIGGSGRRIHILLSH
                     LHWDHIQGFPFFVPAYIPGNRITFYGFHPDIERAFEVQQSAPWFPATLSMMASEKKFV
                     HLEIGKTYGIGNFTVRGIEQEHPGTAYGYSIQHNGKKIVYSTDSEHKDGTPVEESPFV
                     PFFRDADLLIFDAQYSLADADVVKRDWGHSSNILAVEFASLARAKRLVLFHMDPAKRD
                     EDLDRILSETKKYAALFDDSYAFEIDVAYDGMAVDV"
BASE COUNT         1103 a         1667 c         1772 g         1166 t
ORIGIN      
        1 cacggcgatg gggagcggcg gcgtcccctt gagcgtgagc gggatgagca catagaacag
       61 cagcacgatg gtggccacga gcgacaggat cgacagcgcc ccgcgcaatc ggcccacgaa
      121 gacgatcgcg gcgaacatca gcgcgcccag cacgatgagc ccggcggtgt tgtccacgtc
      181 gtaaatggag atctgctcca cgtcctcgcc gccgccgaat ttcgagatgc cgatgtagac
      241 cgtgtccccc tcgcggtaga acatgtcctt gggcatgtcg tcctcgccgc ggtacacggc
      301 cacatgcgtt ttccccttct tggacccctc caggacgcgc agatggatcc gcagctccac
      361 gagcgatgcg cgctcggcgc tggcgctgcg cttctcgatg cgctccacct tcgcgcgcgc
      421 gatctcgcgc tggtcgatat acgggtcgcg ctctttcgcg gacgcggtcg cagcggtcgt
      481 aaacgcgagt gtcgcgagca gcgcgagcag cggccggatc atcattgaag tggggaaggt
      541 cacatcgtat ctcccggtaa ttggcgtgcg cgatcgggcg cgcatatccg caatcgtcgg
      601 tcatgcggtt tgacaaggca agcacttatt ccgggtatga cgccatgtcg gcgaatcgga
      661 catgtcgcga aaacgagcat ccgttcccgt ttggatgaga tgcgacagcg cagtctgctg
      721 atcccgttca gcgggaaaga ccatgtcttt tcgcggcgca gtgttgatgg cgcgatccgc
      781 atgatcgttg gtcccggccg gttttcgtga ttgacacggg ccggcgtgtc tcatatcacg
      841 agcgtgcgct gtgcggccgg gagagccgcg caaggcgcag cagaatcggt gggtcgccga
      901 tgggcgcatg ggaatgccgc gatgcggcgg atgcatactg taagggggaa tcgtggacat
      961 aaccaagaac cgtctctatg ccgtcgttgg cgcgatcgtg ctttttaaac tgattctcgc
     1021 gtacgcattc cccatctggc cggacgaagg atactatctc acctggacca ggaatatcga
     1081 caccggattt tactgccatc ctcccatggt gggctggctc atgtacgtga tgtcgttcat
     1141 cagccgggac atcttctttc atcggctcat cgcgatcttc gtatcgacgg cggctggatt
     1201 cgtgatcttc gcggtcgcgc gcgatatcac ccgcgacgag aaaaaagcgg cgctggcgag
     1261 cctggtgtat ttcctgtcgc cggcgaacgt cttctttatc gtcctcacca acgacgcgcc
     1321 gctgttcctg tttacgctgc tgtgcgtcgt ctgcttctac tacgcggtgg cgcgcgaccg
     1381 gatcatcttc agcatgctcg ccggcgtctt tatgggcctt gcgttcctgt gcaagtactt
     1441 cgcggtgctg ctgttcctgt ccatggtggt ctatctggcg ttcaacagga accggcgaac
     1501 gctcacgcac tgtctcgtcg ccgggatcgc ggcgctgccg atcgcgtcca tcaacctcta
     1561 ttacaactac atgaactgct ggaacaatgt catggtccaa tggttcaacc ggaccggcga
     1621 caagtccttc agcgcgctgt attttatcgg ctacgtgttc gggcagctct actggctcac
     1681 gccgtggggg ctgtattacc tcgttcggga gcggggcggc gtcgcggcgt tcttcgccga
     1741 gcgcacgagg cttgtgttcg ccctcgcgct tctggcgccg ctcgcgttca tggccctcat
     1801 cggcctcaag gcggcgtcgt tgcactggac gctgccgttt ttcagcatcc tgcccatcgt
     1861 gctgctgggg ctccagtcgg aaagtctgaa gaggatcgtc gtcgggaacg cgatcttcgc
     1921 gtcggcgcat gcgctggtga tcgtcgtgct cctcctcctc ccgctttcgc tcttcaaaga
     1981 cttccggtac tataccgact tcgtgctcta caagaagggc gatctcatct gcgcggagct
     2041 cgctccgtac cgcgggaagt acgtcttcgg caccatcggg tatacagtct cctcgacgct
     2101 ggactattac tgcggcgagt atttcctgat attcaactcg ctctccaagt ggggacgcaa
     2161 cgacgacaag gtgaccgact tcaggaagct cgacgggaag aacattctcg tcgtctcgcc
     2221 caagatcgag acggccgacg agtaccgcca cctgtttgag cgcgtcgagg ttcgctatgt
     2281 cgatgtgctc gaggggcgct tctcgttcat cgtcggggac ggcttcaagt acgcggccta
     2341 tcgcgacacc acgctccgga aggcattgga caagctgtac aatgtcccga cctggctgcc
     2401 ctgcgggagc tgctactttt tcgaccgcta ctttccggag cggaagtatt gatgatgcgc
     2461 tgtcgcgctc gccgggaccg gcgtcagatg cacacggacg atagcatctc cgaaagcatc
     2521 gccatcgtcc gatacgcatc ggtctcgacg aaaacctgct tgaggaaggg cgccttcgac
     2581 gcgcgcagga cgaagatggt cttcaggatg gtcatcgaat agcttacaaa acgggtgttg
     2641 ctggtcccgc tgggccgctt caggtatacc accggaatct ccttgatgcg cagactgagc
     2701 cggtgggcgt agtaaaggat gcggatgaag tactcgccgt agccggtgaa cagcgaatcg
     2761 tgccggatgc gttcgaggac gcttttgcgt atcacgaaaa acccggagag attgtctctc
     2821 gttcgcatgc ccagcgccgt gcggatgatc gcattgaacg catagctccc ccagaatcgt
     2881 cccgccgcat tgtccatgcc gccgccgtcg atgaaacggc tccccgcgac gaggtcgtgc
     2941 tcggcgcagt ggcggatgag cacggggacc cgttcggggt cgtggttgaa atcggaatcc
     3001 atcaccagga ggtactctcc gttcgcgcgg ttgatcccgt aggcgatggc gctcgcgagt
     3061 ccccgttcgt gggtacgcac ataacagcgg accgcgtttc ccaggtgtcc gaatgactcg
     3121 gcgatcgccg tatcggtccc gtcggagctg ttgtcgtcaa cgacgatgat ttcggcggat
     3181 acgccttccc gcgcgcagac gtcgatgagc cgcgcgataa gcgggacgat gttgtctttc
     3241 tctcggtagg tcggaagaat gattgaatac gtcatggagg acctctcaga tcattgtatg
     3301 tttgtgattc gcacgctgag tggggagtcc ggagaatcga tcgtcaaaca aaaaaccgat
     3361 agtgttcgtc gcaaatcgaa aaaaacccat gccatgctgt acatcgggag aatgaatgat
     3421 catttcctgc ccggtatcgt gattataagg cccgtgggct gttttcggcg cccgcaccgg
     3481 acccgtaaaa agtgcttgaa atcgtcaccg cccggagggg aatgaaaccg gcactgatct
     3541 attgatactt tctcggtagc gcttcccggc catcacgtgt ctggagcgcc gtaacatcac
     3601 cacatcttca tggaggccgt atgagcattt cgaagaagac cgttcgcgat atcgatttca
     3661 agggcaagcg cgtcatcatg cgcgtcgatt ttaacgtgcc gatgaaggac ggcgtggtcc
     3721 aggacgacac gcgcatcaag gcggcgcttc ccacgatcca gtatgtcctc gaaaagggcc
     3781 cgaagtatct catcctcatg tcgcacctgg gcgatcccaa gaaggacacg cagaaggcga
     3841 aggaaaaggc cgagaaggac ggaaagacct tcgacgagaa gaaattcatg gagggcaagc
     3901 acatgatgcc gccggtggcg gcctacctcg aaaagctcct cgggaagccg gtcaagatcg
     3961 ctcctgcgtg ctttggcccc gaggtggacg cgatcgtgaa cggcctgggc gctggcgacg
     4021 tgctcatgct gggcaatacg cgcatgcaca aggaggagac cgcgaaggag gctggtgatc
     4081 gcgagacaat ggcgaaggcg atggccgcct atgccgacgt atacgtgaac gacgccttcg
     4141 gtaccgcgca ccgtgcgcac gcctccaccg agacgatggc gaagttcctg ccggccgtcg
     4201 cgggcttcct catggaaaag gagctcgagt tcctcgatga taaaatcgtg aagtcgccgg
     4261 tgaagccctt tatcgccgtc gtgggcggcg ccaaggtgtc gtctaagatc gcggtcctgg
     4321 agagcctgct ggggaaggtg agcggcatca tcattggcgg cggcatggcc tataccttcc
     4381 tgaaggcgat ggggaagggc atcgggaaat cgctggtcga ggaggacatg ctcgatacgg
     4441 cgaaggacat catgcagaag gcgtccgcga aaggcgtgaa gattctcctc ccgctcgatc
     4501 atgtggaggc gaaggagttc tcggccgagg ccgaatcgaa gaccacgacg agcgacggca
     4561 tcgacgacgg ctggatgggg atggatattg gccccaagac cctcgcgcaa ttcgagcagg
     4621 aacttgcggg cgcgaagacg gtgttctgga acgggcccat gggcgtcttc gagttcccga
     4681 aattcgccga gggcaccacg aagatcgcgc ggctcctcgc ggggctttcg gcaacgaccg
     4741 tgatcggcgg cggcgattcc gtttccgcgg tcaacaaggc cggcgtggcc gataagatgt
     4801 cgcacatctc cactggcggc ggcgcctcgc tcgagctcgt tgagggcaag gtgctccccg
     4861 gcgtcgcggc gctcaacgat aagtaaattt gtagccattg tgcccggtag gggcagggca
     4921 atgccatgcc cctaccggca attaaacatc caccgccatc ccgtcgtacg ccacgtcgat
     4981 ctcgaacgca tatgaatcgt cgaacagcgc cgcgtacttc ttcgtctccg agaggatgcg
     5041 atcgaggtcc tcgtcgcgtt tcgcggggtc catgtggaag agcacgagcc gcttcgcgcg
     5101 cgcaagcgac gcgaattcca cggcgaggat attgctcgag tgcccccagt cgcgctttac
     5161 gacgtccgcg tcggcaaggg agtactgcgc gtcgaagatg aggagatcgg cgtcccggaa
     5221 gaacggcacg aagggcgatt cctcgacggg ggtgccgtcc ttgtgctcgg agtcggtcga
     5281 atagacgatc ttcttgccgt tgtgctggat ggaatagcca tatgccgttc ccggatgctc
     5341 ctgttcgatc ccgcgcaccg tgaagtttcc gattccatag gtcttcccga tctcaaggtg
     5401 gacgaacttc ttttccgagg ccatcatcga cagcgtggcc gggaaccacg gcgcgctctg
     5461 ctggacctcg aaggcgcgct cgatatccgg atgaaagccg tagaaggtga tcctgttccc
     5521 ggggatatag gccggaacga aaaacggaaa cccctgaatg tggtcccagt gcaaatgcga
     5581 caggaggatg tggatacgcc gtccgctccc gccgatgcgc ataagatggt tcccgagctc
     5641 gcgcaatccg ctccctgcgt ccaggatgat gaaatcatcg tcgtcgccca cttggacgca
     5701 ggcggtgt
//
LOCUS       DAORHF010000240         5708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14381_length_5708_cov_3.711834, whole genome shotgun sequence.
ACCESSION   DAORHF010000240 DAORHF010000000
VERSION     DAORHF010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14381_length_5708_cov_3.711834"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PKJ16_15505"
     CDS             complement(<1..299)
                     /locus_tag="PKJ16_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HNV48448.1"
                     /translation="MTIHACRICGNSFFEGSLLSYRNMPQAAQFLPDAASLSDDSGID
                     LDIRQCSGCGLVQLSGDPVSYYRDVIRAAAFSDEMRSFRIDQFFGFAGKYGLGGK"
     gene            complement(381..1349)
                     /locus_tag="PKJ16_15510"
     CDS             complement(381..1349)
                     /locus_tag="PKJ16_15510"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV48449.1"
                     /translation="MKFTRIAILGATSHIARGLIYHFSSTPWASLFLFARSPERVGSF
                     IAEHGLMSPVEIFDGFESIHLREYDVIINCVGLGSPPKIKNRKNEFFRLTEHYDNMVM
                     DYLDKHPAALYLNMSSGAVYGTDFSQPAEHDRNAILPVNRITADHFYSIAKINAEAKH
                     RAHPEKNIVDLRVFTYFSRYIDIESGYLITEIVKSARDKRKFLTGKSDIIRDIVGPAD
                     LFSLVCLCIGQRTINTSFDVYSAGTARKFEILDFFQNCYGLEYVVDENFSTTDSIGLT
                     LNYYSTDTRAKEIGYVPQYSSLGVIEEEVKSILSGEGGGAEKRHES"
     gene            complement(1354..1917)
                     /locus_tag="PKJ16_15515"
     CDS             complement(1354..1917)
                     /locus_tag="PKJ16_15515"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HNV48450.1"
                     /translation="MNRHSEREHTIDAQDSIRHAIGTLDSAIADPKQGLPEDVFLFIS
                     RITPMINVDLLVKDEKNRVLLTWRQDRYHEPGWHVPGGIVRFKEKFEERIRKVALNEL
                     GAVVSHDVDPVEMNQLIIRDREERSHFISLLFRCTLVTGLDAAREHISGEPVNGFWRW
                     HESCPNTLLAVQSMYRKYFHDAARYHE"
     gene            complement(1917..3722)
                     /locus_tag="PKJ16_15520"
     CDS             complement(1917..3722)
                     /locus_tag="PKJ16_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HNV48451.1"
                     /translation="MIKLSDYVISFIADRGVDRIFMLPGGGCMHLVDSVGRNGRLTFT
                     GCLHEQAAVIAADGYAQFRGGLGVALVTTGPGGTNALTGVAGSWIDSTPVLVISGQVK
                     RKDLLAGKGVRQMGIQEVDIISLAKPITKYAAMIEDPTSVRFHLEKAVHLATTGRPGP
                     VWLDIPLDVQGAMIDETTLAGFTPDAKSVSPYEMASNAGVEKAIALLNEAERPVILAG
                     RGIRLAGALNEYIRLVDALQVPVLTTWRAADFLPEDHPLFFGRPGSIASRYANFVQQN
                     ADLIFIIGARLDLPQVGHGYENFARAARKVIVDIDEAEIRKTDIDADVSVVGDAGFFV
                     RQFLQGIDSVMSRDRSAWLSRCREWKSHFPLVTSEHHAMGDYVNTYAFIDVLSDLLGE
                     NDVIVPGSSGTCAEITLQALRIKAGQRLINCPGLGSMGYGLPQSIGVCLASGGRRTVC
                     IVGDGGLQHNIQELETLKRTKAPVKLFVLNNNAYASIRNTHTRFFEGRLVCCDPSSGL
                     TLPDTCKVAEAYGLPAVRISDQKNLRRDVEEVLGRAGPVVCELMIDPALQTAPRVSSY
                     AKPDGSMASKPLEDLWPFLERDEFRANMLIPLLDE"
     gene            complement(3734..4327)
                     /locus_tag="PKJ16_15525"
     CDS             complement(3734..4327)
                     /locus_tag="PKJ16_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48452.1"
                     /translation="MLTIAWDVDDVLNDLMRTWFEAEWLTRYPLCEISYNDISANPPH
                     GLLGVSQEEYLCSLDRFRQSDAYLAMEPVGPVMEWFRSYGERFRHIAVTAVPRTAAHR
                     SAQWAIGRFGDWIRTFHFVPSARPGATIPAYDRGKGEFLRWLGRADVFIDDDEGNIAS
                     ADGLDVRCLLFPRPWNGAAGKTIEEALDGLSRMLMDK"
     gene            complement(4341..>5708)
                     /locus_tag="PKJ16_15530"
     CDS             complement(4341..>5708)
                     /locus_tag="PKJ16_15530"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HNV48453.1"
                     /translation="TFIHPDYKGRSEFKYVKRFSETRLSEDEFIALKNEMTRLGFVTI
                     CTPFDEESVDMIERHGFDIIKIASCSFTDWPLLERISKTDKPLIASTAGIALLDIDNV
                     VSFFEHREKDFALMHCVGEYPTQARNLDLGQIELFRARYQNVPIGYSTHEDPENMDAV
                     KMAVAAGAIILEKHVGVPKDAYQLNAYSASPGQVDRWLDSALRAFEMRGEAADRRSIT
                     EKERTDLRGLQRGVFVKNPVKAGEKITAGNVFFAIPNLDGQLVANDLSKYSEFMAKRD
                     ITANGPVLFQDVDHRNLRDRVIEIIGKVVNMISASSICLPNKLDVELSHHFGIDRFDE
                     WGATIINLINREYCKKIIILLPGQNHPAHLHKKKEETFHVVYGSLVLSLDGKEREYRE
                     GDIIIVERGMSHSFRSAGGAIFEEISTTHYVDDSFYDDANVMANKHRKTAMTYWSDWL
                     TRPIR"
BASE COUNT         1269 a         1648 c         1519 g         1272 t
ORIGIN      
        1 ttctttccgc caagcccata cttccccgca aaaccgaaaa actggtcaat acggaaggac
       61 ctcatctcat cagaaaatgc cgcggcgcgt ataacatcgc gataatagga taccggatcc
      121 ccgcttaact gcaccagccc gcatccggag cactggcgta tgtccagatc aatacccgaa
      181 tcgtctgaca gcgatgcggc atcaggcagg aactgtgcgg cctgcggcat gttccgataa
      241 ctaagcaacg agccttcaaa aaaactgttt ccgcatattc tacatgcgtg aatagtcata
      301 cgacgcagcc tcacacatct tacagcgaat atccaattgc tgggatttta caccaaaagg
      361 aaaacgataa cgaaataatt tcatgattca tggcgttttt cagctccgcc gccctctccg
      421 gaaagaatgg attttacctc ctcttctatt acgccaagag aggagtattg aggtacataa
      481 ccgatctcct tggctcgggt atccgttgaa taataattca acgtcaaccc aatggaatcg
      541 gtggtactga agttttcatc aaccacatac tcaaggccat agcaattttg aaaaaaatcg
      601 agaatttcga actttctcgc tgtgccagcg ctgtacacat caaaggaggt gttaattgtt
      661 cgctgcccaa tacaaaggca caccagcgaa aagagatcgg ccggccccac gatgtcccgt
      721 atgatatcgc tcttccccgt gaggaatttt ctcttgtccc tggccgattt aactatctca
      781 gttattaagt aaccggactc gatgtcgata taacggctga aataggtgaa cacgcggagg
      841 tcaacaatgt tcttttccgg gtgcgcccga tgcttcgcct ctgcgttgat tttagcgatc
      901 gagtaaaaat ggtcggcggt tattcgattc acgggtagta tcgcatttct atcatgttcg
      961 gccggttgcg agaaatccgt accatacacg gcgccgctgc tcatgttaag gtaaagcgct
     1021 gcggggtgct tatcgaggta atccatcacc atgttatcgt aatgctcggt taaacgaaag
     1081 aattcgtttt tccggttctt tattttcggc ggcgatccaa ggccaacaca gttgattatc
     1141 acgtcgtact cgcgcaggtg aatcgattcg aacccgtcga aaatctctac cgggctcatg
     1201 agaccatgtt ccgcaatgaa actcccgact cgttctggcg accgtgcgaa gagaaaaaga
     1261 gaagcccatg gtgtgctaga aaaatgataa atcaagccgc gggcgatatg gcttgtcgcc
     1321 ccgaggatcg cgattcgagt gaacttcatg ttttcactca tgataacgtg ccgcatcatg
     1381 aaaatatttc ctgtacatac tttgaacggc tagcagagta ttgggacagc tctcatgcca
     1441 ccgccaaaag ccgtttaccg gctctccgga aatatgttcg cgcgcggcat ccaatcccgt
     1501 cactaacgta cacctgaaca gtagggagat gaaatggctg cgctcctctc gatcgcggat
     1561 tatgagctgg ttcatctcaa ccgggtcgac atcatggctt acgacggcgc cgagctcatt
     1621 gagggcgact ttacgtattc tctcctcgaa tttttcctta aaacgtacaa tcccgccggg
     1681 aacatgccat ccgggctcat gatatcgatc ctgccgccat gttagcagga cacgattttt
     1741 ctcgtcttta acgagcaaat cgacatttat catcggtgtt attctggata tgaaaagaaa
     1801 aacatcctcg ggcaatccct gcttcggatc ggcaatagct gaatcgagcg tcccgatcgc
     1861 atgtcgaatt gaatcctgtg catcaatagt gtgctcgcgc tcactatgtc tattcatcat
     1921 tcatccaaca gcggaatcag catgttcgcc ctgaactcat cgcgttccag gaacggccat
     1981 agatcctcaa gcggttttga ggccatcgag ccgtcgggct ttgcgtatga cgacacgcgc
     2041 ggggccgttt gcagcgcggg atcgatcatg agctcgcata caaccggccc cgctctcccg
     2101 aggacctctt cgacatcgcg gcgaagattt ttttgatcgg aaatcctcac cgcgggtaat
     2161 ccgtacgcct cggcgacctt gcacgtgtcc ggaagggtca agccgctgga gggatcgcag
     2221 cacacgagcc ggccctcgaa aaagcgggta tgcgtgttcc ggatcgaggc gtaggcgtta
     2281 ttgtttaaga cgaacagctt caccggcgcc ttcgttcgct tgagcgtttc cagctcctgg
     2341 atattgtgct ggaggccgcc gtcgcccacg atgcacacgg tgcgcctccc accgctggct
     2401 aggcacacgc caatgctttg gggcagccca tagcccatgg aaccgagacc gggacagttg
     2461 atcaaacgct gcccggcttt aatgcggagt gcctgaaggg tgatctcggc acaggtgcca
     2521 gaactacctg ggacaatcac gtcgttttcc ccgaggagat cggacaggac gtcaatgaaa
     2581 gcataggtat tcacgtaatc gcccatggca tggtgctcag acgtgacaag cgggaaatgg
     2641 gatttccatt cacggcagcg cgagagccac gctgagcggt ctctgctcat gacggaatcg
     2701 atcccttgaa ggaactgccg gacgaagaag ccggcatcgc cgacaaccga cacgtctgca
     2761 tcaatgtcgg ttttgcggat ctccgcttcg tcgatatcga caatcacctt ccgcgctgcc
     2821 cgggcgaaat tctcatatcc gtgccccacc tgggggagat cgagacgtgc gccaataatg
     2881 aatatcaaat cggcgttctg ctggacaaag ttggcgtagc gggaggcgat ggaccccggc
     2941 cgtccgaaaa agaggggatg atcctcgggc aggaaatccg cggcgcgcca ggtagtcagg
     3001 acggggacct ggagcgcatc gacgagccgt atgtactcat tgagggcacc ggcgagcctg
     3061 atgccccgtc ccgcaaggat aacaggccgc tccgcctcgt tgagcaatgc aatcgctttc
     3121 tcaacgccgg cgttactagc catttcataa ggcgacaccg atttcgcatc gggcgtgaag
     3181 cccgcaagcg tcgtctcgtc tatcatggcg ccctgcacat cgagggggat atcgagccac
     3241 accggtccgg gccgtcccgt tgtggccaga tggaccgctt tttcgagatg gaagcgcacc
     3301 gaggtcgggt cttcaatcat cgccgcgtac ttggtaattg gtttcgcaag cgatataata
     3361 tcgacctcct gaatgcccat ctggcggacg cccttgccgg ccaggaggtc ctttctcttc
     3421 acctgcccgg aaatgaccag tacgggggtt gaatcgatcc aggaaccggc cacgccggtg
     3481 agcgcgttgg tccctcccgg cccggttgtc acgagggcga cgccgaggcc cccgcggaac
     3541 tgcgcgtaac cgtccgcagc gatcaccgcc gcctgctcat ggaggcagcc ggtaaacgtc
     3601 aggcgcccgt tgcgccccac cgaatctacc agatgcatgc atccgccgcc gggcagcata
     3661 aaaatccggt ccacgccgcg atcggcgatg aaagagatta catagtcgct aagttttatc
     3721 atatcgtgtc gcttcacttg tccataagca ttctcgagag gccgtccagg gcctcttcaa
     3781 tcgtcttccc agccgcgccg ttccatgggc gggggaagag cagacaccgc acgtccaatc
     3841 catccgccga ggcgatattg ccttcgtcat cgtcaataaa gacgtcggcc cgtccaagcc
     3901 accggagaaa ctctcccttg ccgcgatcat acgccgggat tgtcgcgccg gggcgggcgg
     3961 agggaacgaa atgaaacgtt cgtatccagt cgccaaagcg gcctattgcc cactgggcgc
     4021 tccggtgcgc cgccgtacgc gggaccgccg ttacggcaat gtggcgaaac cgttcgccat
     4081 agcttctaaa ccactccatc acgggcccta ccggctccat cgcgaggtac gcgtccgact
     4141 gccggaagcg gtccagggaa cagagatatt cctcctgcga aacccccagg agaccgtggg
     4201 gcgggttcgc ggaaatatcg ttatacgaaa tctcgcacaa cgggtaacgc gtgagccatt
     4261 cggcctcaaa ccacgttcgc atgagatcgt tcaggacgtc gtccacgtcc caggcgatgg
     4321 tcaacacgcg gaggctcccg tcaccggatg ggccgtgtaa gccagtcgga ccagtaggtc
     4381 atggcggtct tccggtgctt gttggccatg acgttcgcgt cgtcatagaa cgaatcgtca
     4441 acgtaatggg tcgtcgatat ctcctcgaag atcgcgcccc ccgccgacct gaagctatgg
     4501 ctcatgcccc gctcgacgat gatgatatcg ccctcccggt attcccgctc cttcccgtcg
     4561 agagacagca ccaggctgcc gtacacgaca tggaacgtct cttccttttt cttgtgcagg
     4621 tgcgccgggt ggttttgccc gggaagaagg atgatgatct tcttgcagta ttcacggtta
     4681 atgaggttga tgatcgtggc gccccactca tcgaagcggt caatcccaaa gtggtgcgac
     4741 agctccacat cgagcttgtt cgggaggcag atgctgctcg cggagatcat gttcacgacc
     4801 ttcccgatga tctcgatcac gcggtcgcgc aggtttcggt gatccacgtc ttgaaacaaa
     4861 accggaccat tggcggtgat gtccctttta gccatgaact cgctgtattt cgataggtca
     4921 ttcgcaacga gctgcccgtc gagatttggt attgcgaaaa agacattacc ggcggtaatc
     4981 ttctcgcccg ctttgacggg gttctttaca aatacccccc gttgcaaccc gcgcagatcg
     5041 gtccgttcct tctctgtgat gctcctccgg tcggcggcct cgccgcgcat ctcgaaggcc
     5101 cgcagcgccg aatcgagcca ccggtcaacc tggccgggac tggccgagta tgcgtttaac
     5161 tgatatgcgt cttttgggac gcctacatgt ttttcaagta ttatcgcccc cgccgccacc
     5221 gccatcttca ccgcgtccat gttctcgggg tcctcgtgtg tcgaataacc tatcggcacg
     5281 ttctggtagc gcgctcggaa gagctcgatt tgcccgaggt caaggtttcg cgcctgtgtg
     5341 ggatattcgc ccacgcagtg cataagagca aaatccttct cccggtgctc gaaaaagctc
     5401 accacgttgt cgatatcgag gagcgctatg ccggccgtcg atgcgatgag aggcttgtcg
     5461 gttttggaga tccgctccag gagcggccag tcggtaaacg agcaactggc aatttttatg
     5521 atatcgaagc cgtgccgctc gatcatgtcc accgattctt catcgaacgg cgtgcagatg
     5581 gtaacaaagc cgaggcgcgt catctcattc ttgagcgcga taaactcgtc ctcggagagc
     5641 cgtgtctcgg agaagcgctt cacgtacttg aattcgctcc tccctttata atcgggatga
     5701 atgaaagt
//
LOCUS       DAORHF010000241         5706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14391_length_5706_cov_4.107769, whole genome shotgun sequence.
ACCESSION   DAORHF010000241 DAORHF010000000
VERSION     DAORHF010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14391_length_5706_cov_4.107769"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="PKJ16_15535"
     CDS             complement(<1..1075)
                     /locus_tag="PKJ16_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV48454.1"
                     /translation="MIYQQSCETMPRDELEQLQIERLQMTLNRVYRNVAFYRKGFDAA
                     GVDIGKIRSVGDLKELPFTTKEDLHASYPYDMFAVPLQDIVRIHSSSGTTGRPIVVGY
                     TKNDIQHWSALTARVLTAAGITEHDFVQIAFEYSLFTGGLGFHYGAERIGASVIPSSA
                     SGNIQKQLYIMKDYKTSALLSTPSFAITIASNLREMGIHPESLNLRVGIFGAEPWSDA
                     IRARIEDELRLDAYDSYGLSEIMGPGVSGECAHKCGLHVNEDHFIVEVIDPATGAPLG
                     YGAEGELVFTTITKEGVPLIRYRTGDLGTLTGGTCECGRTFARMSRVSRRIDDMIIVR
                     GVKIVPRQIEEILLRVEGARPNYQ"
     gene            complement(1072..1830)
                     /locus_tag="PKJ16_15540"
     CDS             complement(1072..1830)
                     /locus_tag="PKJ16_15540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48455.1"
                     /translation="MLRIRNLHAGYENLLVIKGASLHVNPGEIVTIIGANGAGKTTLL
                     QTLAGLVRPREGEISFDKTSITALPAERIVGMGCVLVPEGRQVFGTLTVEENLFLGSY
                     SRRRSLPREEYRRELGDVYRLFPRLEERSGQLAGTLSGGEQQMLAIGRALMAGPRLIM
                     LDEPSMGLAPIIVKTIFEVLARLRDMGKTILLVEQNAKAALRIADRGYVMETGQIILE
                     ESAAELLNNKDVQRAYLGKEYKSIADLGEQGERQ"
     gene            complement(1830..2588)
                     /locus_tag="PKJ16_15545"
     CDS             complement(1830..2588)
                     /locus_tag="PKJ16_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015402376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48456.1"
                     /translation="MSFFAVENLSRAFGGLQAVSDVSFAVREHSIHAVIGPNGAGKTT
                     LFNLIAGSLAPDSGAIRLNGDPLQSLPPYKIARKGIARTFQNVKLFRHMSVLENVMLG
                     RHVRSRAGFGAALLNLPHTWREERSIRQRSLEILDTLGIADLADADAVSLAFGKQRIV
                     EFARALATEPTLLLLDEPAAGLNMHETDEIARLIADIRARGITVLIIEHDMSLIMDIS
                     DHIAVLSSGKKIAEGTPAEIQRNRDVIAVYLGED"
     gene            complement(2585..3577)
                     /locus_tag="PKJ16_15550"
     CDS             complement(2585..3577)
                     /locus_tag="PKJ16_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV48457.1"
                     /translation="MNIRQYIPAGILVAVVVAFQLAVVLTGQDYLLTQLTMSAYYALV
                     IIGLSVLMGYAGQISIGHAAFFAIGGYTAAVLTTGAFRLSPWLSLAVAVAITVVIAFL
                     IGIPILKLKGHYLAMATLSFGVIVYRIVLGTGVFGEADGISDVPGFTLLPGLTVTGGA
                     ASRVENYYIAWFLVTLAIILLINLINSRVGRALRSIHGSEEAANAMGVNTAGYKLAIF
                     VLSAVFAAVGGVFLTHYTGGIGPSEAGVMKSVRYVAIVAVGGMANIWGALVMGIVLNY
                     LSLRGYFGTYDDIFFGAILIIIMLFYPDGILQRKTLDDLRSLARSLVARIRGRR"
     gene            complement(3764..4654)
                     /locus_tag="PKJ16_15555"
     CDS             complement(3764..4654)
                     /locus_tag="PKJ16_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HNV48458.1"
                     /translation="MTFEHVLQYLLAGITIGSIYAIVAIGFNIIYNTTGIINFAQGEF
                     LMVGAMTAVTFSRLVPLPLAIAIGIAVTVAVGVLIDFVFIRNLKKPTVLNMIIVTIGL
                     SIIIREVALHVWDEKVRSLPYFTGDEISSINILGAYISPQALWVLGICATMVVLLNVF
                     FKYSVMGRSMRACSANPVAARLCGINTKNMVTMSFVLSAAIGALAGCVMSPITQTQYD
                     CGTSLAIKGFTVAILGGLGNSTGAIIGGLIVGILEEFSISVMPLAYKDAIAIAILLLI
                     MFFRPSGLFGKQEVSALKEF"
     gene            complement(4745..>5706)
                     /locus_tag="PKJ16_15560"
     CDS             complement(4745..>5706)
                     /locus_tag="PKJ16_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HNV48459.1"
                     /translation="INGKKIELIIKDTGSSPEKAVSFAKQLIEEEKVLAIVGPSTSGE
                     TMKIKSICDNAKVILISCAAAEVIVNPVAKYVFKSPQNDSQAAEKIFDTMKQMGITKI
                     GIINDNGGFGIAGRDQLKKLAPKKGITILASEEYDSKATDLTGVLTKLAAKKVQAVVN
                     WSIVPAQSIVPKNMKQLNMNVPLFQSHGFGNIKYVKEAGKAGEGIIFPCGRLLVADQL
                     PDNHPQKALLVKYKKDYESKYNEEVSTFGGHAYDAITILFEGIKKAGVAETDKVRDAI
                     ENLKGFAGTAGIFKFSATDHCGLDINAFEMLTVKDGKFVLYKK"
BASE COUNT         1048 a         1820 c         1784 g         1054 t
ORIGIN      
        1 cctgatagtt gggacgggcc ccctcgaccc gaaggaggat ctcctcgatc tgccgcggga
       61 cgatcttcac gccccggacg atgatcatgt cgtcgatccg ccgcgaaacg cggctcatgc
      121 gcgcgaaggt ccgaccgcat tcgcaggtcc cgcccgtgag cgtccccaga tcgcccgtgc
      181 ggtatcggat caggggcacg ccctccttcg tgatggtggt gaacaccagc tcgccctcgg
      241 ccccgtatcc gagcggcgcg cccgtggccg ggtctatcac ctcgacgatg aagtggtcct
      301 cgttcacgtg aagcccgcac ttgtgcgcgc attcccccga cacgccgggc cccatgatct
      361 cgctcaagcc gtagctgtcg taggcgtcca gacgcagctc gtcctcgatc cgcgcgcgga
      421 tcgcatcgct ccagggctcc gcgccgaaga tgcccacacg gagattgagc gactccggat
      481 ggatgcccat ttccctgagg ttcgacgcaa tggtaatggc gaagctcggc gtggacaaaa
      541 gcgccgaggt cttgtagtcc ttcatgatgt acagctgctt ctggatattg ccgctcgcag
      601 aggacgggat cacggaggcc ccgatgcgtt cggcgccgta atggaacccc aggccgccgg
      661 taaagaggct gtactcgaag gcgatctgca cgaagtcgtg ctcggtgatc ccggcggccg
      721 tgagcacgcg cgcggtgagc gccgaccagt gctggatgtc gttcttcgtg tatcccacca
      781 cgatgggccg cccggtagtc cccgacgagg agtggatgcg gacgatgtct tgcagcggca
      841 ccgcgaacat gtcgtaggga tagctggcgt gcaggtcctc cttcgtggtg aacggcaact
      901 ccttgaggtc gcccaccgaa cgaatcttcc cgatatcaac cccggcggca tcgaagccct
      961 tccggtagaa cgccacattc cggtacaccc ggttgagcgt catctgcaag cgctcgatct
     1021 ggagctgctc cagctcgtcg cgcggcatgg tttcgcatga ttgctggtag atcattggcg
     1081 ttccccctgt tccccgagat cggcgatgct tttgtattcc ttgccgaggt aggcgcgctg
     1141 cacgtccttg ttgttcaaca gctcggcggc gctctcctcc aggatgatct ggcccgtctc
     1201 catcacatag cctcggtcgg cgatgcggag ggcggccttg gcgttctgct cgaccaggag
     1261 aattgtcttg cccatgtcgc gaaggcgcgc caggacctcg aagatcgttt tcacgatgat
     1321 gggcgcgagg cccatcgacg gctcgtccag catgatgagg cgcgggcccg ccatgagcgc
     1381 gcggccgatg gcgagcatct gctgctcgcc gccggagagg gtgcccgcga gttgcccgga
     1441 gcgctcttcg agccggggaa agagccgata gacatcgccc agctcgcggc ggtactcctc
     1501 gcgcggaagc gagcgacggc gcgagtacga tccgaggaag aggttctcct ccaccgtaag
     1561 cgtgccgaac acctggcgcc cctccggcac gagcacgcag cccatgccca cgatccgctc
     1621 ggccggcagc gcggtgatcg aggttttatc gaaactgatc tcgccctcgc ggggacggac
     1681 aaggcccgcg agcgtctgga gcaatgtcgt ctttcccgca ccgttcgcgc cgatgatggt
     1741 cacgatctcg ccgggattca cgtgcaacga ggcccccttg atcaccagca ggttctcgta
     1801 gcccgcgtgc aggttcctga tccgcagcat cagtcctccc ccaggtacac cgcgatcacg
     1861 tcccggttgc gctggatctc ggccggcgtt ccctcggcga tcttcttccc ggaactcaag
     1921 accgcgatat ggtcggagat gtccatgatg agcgacatgt catgttcgat gatgagcacg
     1981 gtgatcccgc gcgcgcggat gtcggcgatg agccgcgcga tctcgtcggt ctcgtgcatg
     2041 ttgagccccg cggcgggctc gtcgagcagg aggagcgtcg gctcggtcgc gagcgcgcgg
     2101 gcgaactcca cgatccgctg cttgccgaag gccaggctca cggcgtctgc gtcggcgagg
     2161 tcggcgatgc ccagcgtgtc cagtatctcg agcgagcgct gccggatcga ccgctcctcc
     2221 cgccaggtgt gcggcagatt gaggagcgcg gcgccgaacc ccgcgcgact gcggacatgg
     2281 cgtcccagca ttacgttctc cagcacggac atgtgccgaa acagcttcac gttctggaag
     2341 gtgcgcgcga tccccttgcg ggcgattttg tacggcggga gcgactgcag gggatccccg
     2401 ttcaatctga tggcgccgct gtcgggcgca agacttcccg cgataagatt aaacagcgtc
     2461 gtcttgcccg cgccgtttgg cccgatgacc gcgtgaatgc tgtgttcgcg cacggcgaag
     2521 ctcacatcgc tcaccgcctg caggccgccg aaggcccgac tgaggttctc cacggcaaaa
     2581 aagctcatcg gcgcccccgg atgcgcgcca caagcgagcg cgcgagcgag cgcaggtcgt
     2641 ccagcgtctt tcgctgcaaa atcccatcgg gataaaacaa cataatgatt atgaggatgg
     2701 cgccgaagaa gatatcgtcg taggtgccga agtacccccg aagcgaaagg tagttgagga
     2761 cgatccccat caccagcgcc ccccagatgt tcgccatgcc gcccaccgcc acgatcgcga
     2821 catagcgcac cgacttcatc actcccgcct cggagggccc gatgccgccg gtatagtgcg
     2881 tgaggaacac gccccccacc gccgcgaaca ccgcgctcag gacgaagatg gcgagcttgt
     2941 agccggcggt gttcacgccc atcgcgttcg ccgcctcctc gctgccgtgg atcgaacgga
     3001 gcgccctccc cacgcgggaa ttgatgagat tgatgagcag gatgatggcc agcgtcacca
     3061 ggaaccaggc gatgtagtaa ttctccaccc gcgacgccgc cccgcccgtg accgtgagtc
     3121 ccggaaggag cgtaaagccg ggcacgtcgg agatgccgtc cgcctcgcca aagacgcccg
     3181 tacccaggac aatccggtaa acgattacgc cgaaactgag cgtcgccatc gcgagatagt
     3241 gccccttgag cttgaggatt gggatgccga tgaggaaggc gatcacgact gtaatggcca
     3301 cggcgacggc aagcgacagc cacggcgaga gccggaacgc ccccgtggtg agcaccgcgg
     3361 cggtgtaccc gccgatggcg aagaaggccg catggccgat ggagatctgc ccggcgtacc
     3421 ccatgagcac cgacaggccg atgatgacca gcgcgtagta cgccgacatc gtgagctgcg
     3481 tgagaagata gtcctgcccg gtgagcacga ccgcaagctg gaaggcgacc acgacggcga
     3541 caaggatgcc ggccgggatg tactgtcgta tgttcatcgt tgatagaaat ccgccccgac
     3601 accggaacca ctgccgccat cgagcgcgcg acccggcaga cggtcatgtc gatacggcga
     3661 gcgcgatccg ccgtcgaaac gcgaggcgca cgatggcgcc gtgtgcgacg gcgcgcgcat
     3721 gagtctctgt cgcatgatgc ggatcatccg gtgaaaatgc ctcctagaat tccttgaggg
     3781 cgctcacctc ttgcttcccg aacaggccgc tcggccggaa gaacatgata agaagcaaga
     3841 tggcgatggc gatggcgtcc ttgtaggcga gcggcatcac gctgatgctg aactcctcga
     3901 ggatgcccac gatgagcccg ccgatgatgg cgccggtcga gttccccagc ccgccaagga
     3961 tcgcgaccgt aaaccccttg atggccagcg acgtcccgca atcgtactgc gtctgggtga
     4021 tgggcgacat tacgcagccg gcgagcgccc cgatggccgc gctgaggaca aaggacatcg
     4081 tgaccatgtt cttcgtgttg atgccgcaca ggcgcgcggc gaccggattg gccgaacagg
     4141 cgcgcatgga gcggcccatc accgaatact tgaaaaacac gttgagcagc acgaccatcg
     4201 tcgcgcagat gccgagcacc cacagcgcct gcggcgaaat gtaggccccg agaatgttga
     4261 tggatgatat ctcgtcgccg gtgaaatacg gcagggagcg gaccttctcg tcccacacgt
     4321 gcagggccac ctcgcggatg ataatggaca gcccgatggt cacaatgatc atgttcagga
     4381 ccgtgggttt tttaaggttt ctgatgaaga cgaagtcgat gagcaccccc accgcgacgg
     4441 tcacggcgat cccgatcgcg atggccagcg gcagcggcac caggcgcgag aaggtgaccg
     4501 cggtcatcgc gcccaccatg agaaactccc cctgggcgaa gttgatgatg cccgtggtgt
     4561 tgtagatgat gttaaacccg atcgcgacga tcgcgtaaat gctcccgatc gtgatccccg
     4621 cgagaaggta ctgcaaaaca tgctcgaagg tcatggatgc ccgccgtgaa ctgccctctc
     4681 ggaacgggag ggcgaatgga gtgcgccgcc tcccccgtct cagggagagg cggacgccgg
     4741 tgcgctactt cttgtacagt acgaacttcc cgtccttgac cgtcagcatc tcgaacgcgt
     4801 tgatgtcgag accgcagtga tcggtggccg aaaatttgaa aatcccggcc gttcccgcga
     4861 accccttgag gttctcgatc gcgtcgcgga ccttgtcggt ttcagcgacg cccgccttct
     4921 tgatgccctc gaagaggatc gttatggcgt cgtaggcgtg gccgccgaag gtgctcacct
     4981 cttcgttata cttcgactcg tagtccttct tgtacttcac gagaagcgcc ttctggggat
     5041 gattgtccgg aagctggtcg gcgacaagca gccgcccgca ggggaagata atgccctcgc
     5101 ccgccttgcc ggcctctttc acgtacttga tgttcccgaa accatggctt tggaagagcg
     5161 gcacgttcat gttgagctgc ttcatgttct tggggacgat ggactgcgcg ggaacgatgg
     5221 accagttgac caccgcctgc accttcttgg ccgcgagctt ggtgagcacg cccgtgaggt
     5281 cggtcgcctt gctgtcgtac tcctcgctgg cgaggatggt gatgcccttc ttgggggcca
     5341 acttcttgag ctggtcgcgc ccggcgatcc cgaacccgcc gttgtcgttg atgatgccga
     5401 tcttcgtgat gcccatctgc ttcatcgtgt cgaagatctt ctcggccgcc tggctgtcgt
     5461 tttgcggcga cttgaagaca tacttcgcaa ccgggttcac gatcacctcg gccgctgcgc
     5521 aggagatgag gatcaccttc gcgttgtcgc agatgctctt gatcttcatg gtctcgccgc
     5581 tggtggacgg cccgacgatg gccagcacct tctcctcctc gatgagctgt ttcgcgaacg
     5641 aaacggcctt ctcggggctg gacccggtgt ccttgatgat cagctcgatc ttcttcccgt
     5701 tgatgc
//
LOCUS       DAORHF010000242         5695 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14429_length_5695_cov_3.862943, whole genome shotgun sequence.
ACCESSION   DAORHF010000242 DAORHF010000000
VERSION     DAORHF010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14429_length_5695_cov_3.862943"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..476
                     /locus_tag="PKJ16_15565"
     CDS             45..476
                     /locus_tag="PKJ16_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48460.1"
                     /translation="MEGQRNRTTLIVVAGVLFAIYCLVFGDSGIITRARLAGRKAALQ
                     DRIARQEGEGARLQELLAKYRRGESLRDEAYRAGFIEPGSAVLFFGGALDREAPERMA
                     EDAGRKEAPVDITHLRVLWAVISAVVIMLIVVKQKNESRTE"
     gene            493..1059
                     /locus_tag="PKJ16_15570"
     CDS             493..1059
                     /locus_tag="PKJ16_15570"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HNV48461.1"
                     /translation="MHITLQQTGASDIAVVKWQLGSDSVSIRAIIERCEYGHPRIVLL
                     DPLGGAGPDAFNYESVANVMWLTCPYLNERIHAIESDGMVSKVAEFINQDRALATSMS
                     GAHAHFYFFRKNLFESLFHGSIPSDRLELFDAGIGGIRDTDHIKCLHMHFAHYRICER
                     NIAGYVTNRLLGGKINCDGEDCRHATDR"
     gene            1043..1699
                     /locus_tag="PKJ16_15575"
     CDS             1043..1699
                     /locus_tag="PKJ16_15575"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HNV48462.1"
                     /translation="MPRTAEPKIIRAKKLKKDGSLDGKVVRRIAAALRNGETVLLPVD
                     GVYTLACASRAELEKRHAPARSGGVVYLVSSFKMLADIASFSKADYDFLNRIWPGEVN
                     VTVRPCGDVSGASSCAARFPHNRFIVQVVELAECPIASLCAPGERESVLFHEQDLLAE
                     YRRSADLVVIVEELCKRHPMPTVIDICNGELRIVTEGKVPVDEIKSLYFLGKDDEAVY
                     "
     gene            complement(1721..2527)
                     /locus_tag="PKJ16_15580"
     CDS             complement(1721..2527)
                     /locus_tag="PKJ16_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48463.1"
                     /translation="MTARSKTEETAFTDYAEFWHHNIDKIDEINQFIEKGLARLERQY
                     ADFQEVYDEIIARIDTLAAIYSNYNSLPGREYLLRLKALMRDYEKRYALEGVDFNAIH
                     FLHARIIELKNRQFESFPAIAHEPEAALPPPAPSAPARDIATYPYRWITFEHGLTWYI
                     ARFDEVRVLAARKLAGVERAGSRYRALLDTDTSLITDNAAPAPPLDETIPRFFLVISH
                     NGSERCLAADAAGKRILARRDIISPRLTPLTDAPRSPRYVRIFGRKHFPV"
     gene            2809..4098
                     /locus_tag="PKJ16_15585"
     CDS             2809..4098
                     /locus_tag="PKJ16_15585"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015731776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HNV48464.1"
                     /translation="MVKDAILRYDGREYALPVVEGSEGERAVDIANLRNDSGLITLDV
                     GYQNTGSCKSAITFIDGDRGILRYRGYPIEELAELSTFPETAFLLIYGHLPSREERAV
                     FSQRLTENSMIHEEMLRFFDGFPLTAHPMAILSTMVNALAVYYPRYFTDDWEVATFDL
                     MAARLISKIRTIAAYSYKHSIGEPVVYPRHDLNYCANFLYMMFNSPVKPYDMDEDIVK
                     ALNALLILHADHEQNCSTSTVRLVGSSMVNLYSSICAGICALWGPLHGGANQQVIDML
                     EGIRDQGLTVRKCIEQAKDKNSPFRLMGFGHRVYKNYDPRARILKDKLDLLVRKFNVK
                     EPLVDIALELEQAALTDDYFIERKLYPNVDFYSGILYRIMGIPSDMYTVLFAIGRLPG
                     WIAQWKEMHDTLPFRIGRPRQVYTGETLRSYVPIDAR"
     gene            4416..4967
                     /locus_tag="PKJ16_15590"
     CDS             4416..4967
                     /locus_tag="PKJ16_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013910374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HNV48465.1"
                     /translation="MREIRFHGRGGQGAVTSAELIAISAINKGGFAQAFPSFGPERRG
                     APVVAFCRVDDKKIHIRAKVYNPDVVVVLDPGLVDLLDPTEGMKEGDMLIINTKKTAD
                     QIKGLKNTSIKIATVDANKIAQEELGRVIVNTTMIGAFIKATGIISIDDITEPMMERF
                     GAKLGERNMKALKRAYQEVTIKG"
     gene            4972..5265
                     /locus_tag="PKJ16_15595"
     CDS             4972..5265
                     /locus_tag="PKJ16_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV48466.1"
                     /translation="MAKPIDEITWQELGIGAVVTEIGNSVQYKTGSWRSERPVVKKEH
                     CIKCGVCWLFCPDMSIVQTPEGHFTANLEFCKGCGICAKECPVGCITMQGEEA"
     gene            5267..>5695
                     /gene="porA"
                     /locus_tag="PKJ16_15600"
     CDS             5267..>5695
                     /gene="porA"
                     /locus_tag="PKJ16_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HNV48467.1"
                     /translation="MSEIGKGRKGIEVSIAMADAVAQADVDVIAAYPITPQTHIVEHL
                     ADIVANGELDAEFVHVESEHSAMSVCCGSAAVGARTFTSTSSQGLALMSEIFIIAAAM
                     RLPMVMALANRSLSSPLSIWNDHTDVMMVRDAGWIHFFVEN"
BASE COUNT         1116 a         1639 c         1804 g         1136 t
ORIGIN      
        1 gggaatgatg gcgcggtatt ctgggaacgg ggtcttttac agcattggaa ggtcaaagaa
       61 ataggacgac gctgattgtg gtcgcgggcg tgctcttcgc gatctattgc ctggtcttcg
      121 gcgacagcgg catcatcacc cgcgcgcggc ttgctggacg gaaggccgcg ctgcaggacc
      181 gcatcgcccg ccaggagggc gagggggcgc ggctgcagga gcttctcgcg aagtatcgac
      241 ggggcgagtc gctgcgcgac gaggcgtacc gcgcgggctt tatcgagccc ggaagcgcgg
      301 tcctgttctt cggcggcgcg ctggaccggg aggccccgga gcgcatggcc gaggacgcgg
      361 ggcggaagga agcgccggtt gacattacgc acctgcgggt cttgtgggcc gttatctcgg
      421 ccgtcgtcat catgcttatt gtcgtaaaac agaaaaacga gtcccgaacc gagtagtccg
      481 ttcattgcgt caatgcatat aacactccaa cagacaggcg cgtccgatat tgccgtcgtc
      541 aagtggcagc tgggaagcga ttccgtttcc atacgcgcga taatcgagcg ctgcgaatac
      601 ggccatccgc gcatcgtgct gctcgatccg ctcggcgggg cgggccccga cgcgttcaac
      661 tacgaatcgg tcgccaacgt tatgtggctc acctgtccct atctcaacga acgcatccac
      721 gccatcgagt ccgacgggat ggtctccaag gtggccgaat tcatcaacca ggaccgcgct
      781 ctggcgacca gcatgtcggg cgcgcacgcc cacttctatt ttttcaggaa gaaccttttc
      841 gagagcctgt ttcacggatc gatccccagc gaccgccttg agctgttcga tgcgggcatc
      901 ggcgggatcc gcgacacgga ccacatcaag tgcctgcaca tgcacttcgc gcactatcgc
      961 atctgcgagc gcaatatcgc cggatacgtg accaaccggc tcctgggcgg aaaaatcaat
     1021 tgcgacgggg aagactgccg gcatgccacg gaccgctgag ccgaagatca tcagggcgaa
     1081 aaagctcaag aaggacggtt cgctcgacgg aaaggtcgtc aggcgcatcg ccgcggcgtt
     1141 gcgcaacggc gagacggtcc tgcttcccgt ggacggggtg tacacgctcg cctgcgcctc
     1201 gcgcgcggag ctcgagaagc ggcacgcccc cgcgcgttcc ggcggcgtcg tctatctcgt
     1261 ctcgtcgttc aagatgctcg ccgacatcgc cagcttctcg aaggccgatt acgacttcct
     1321 gaaccgtatc tggccgggcg aggtgaacgt gacggtgcgg ccctgcgggg acgtgtcggg
     1381 cgcgtcatcc tgcgcggcgc gcttccccca taatcgattc atcgtccagg tcgtcgagct
     1441 tgccgaatgc ccgatcgcgt cgctgtgcgc gccgggcgag cgcgagtcgg tcctgttcca
     1501 cgagcaggat cttctggccg agtatcggcg cagcgccgat ctcgtcgtca tcgtggagga
     1561 gctgtgcaag cgccatccga tgcccaccgt catcgacatt tgcaacgggg agctgcgcat
     1621 cgtcaccgag ggcaaggtcc cggtcgacga gatcaagtcg ctgtacttcc tgggcaagga
     1681 cgacgaggcg gtctactgac gcgcgcggga cgcatcccgc tcaaaccgga aagtgtttcc
     1741 tgccgaaaat gcggacatac cgcggggagc gcggcgcgtc ggtcaacggc gtaaggcggg
     1801 ggctgatgat atcgcgtcgc gcgaggatgc gcttgccagc cgcgtcggcg gccaggcagc
     1861 gctccgaccc gttgtgcgaa atgaccagaa agaagcgtgg gatcgtctcg tccagaggcg
     1921 gggccggcgc ggcgttgtcc gttatgaggg acgtgtcggt gtccaggagc gcgcgatagc
     1981 ggctcccggc gcgctcgacg ccggcgagtt tccgggccgc cagcacccgc acctcgtcga
     2041 accgcgcgat gtaccacgtg agcccgtgct cgaaggtgat ccaccgatac gggtaggtcg
     2101 cgatgtcgcg cgccggcgcg gacggggccg gcggcggaag ggcggcctcc ggctcgtgcg
     2161 cgatggccgg aaagctctcg aactgccggt ttttcagctc gatgatgcgc gcgtgaagga
     2221 agtggatcgc gttgaaatcc acgccctcga gcgcatatcg cttctcgtag tcgcgcatga
     2281 gggccttgag gcgcagcagg tattcccgtc ccgggagact gttgtagttt gaatagatgg
     2341 cggcgagcgt gtcgatccgc gcgatgatct cgtcgtagac ttcctggaag tcggcgtatt
     2401 gccgctcgag tcgcgcgaga cccttctcga tgaattggtt tatctcgtcg atcttgtcga
     2461 tgttgtggtg ccagaattcg gcgtaatcgg taaacgcggt ttcttctgtt ttgctgcgcg
     2521 ctgtcatgtc ggtgccatgc gggaggcgtg tcgctctggg tatagtatcg gcgcgggtgg
     2581 gccaattcaa tcatatttcg ccggatcaac cgtgaatttt ccgcctgatc ggtccggacg
     2641 ccacgatcac ccgcgcagga ccatgtccgg cggcggaatc ggcgccgcaa taaatggctg
     2701 gactttcgac ggacccgatg atgtaatgcc gtagcgtgcg cgccctgcgc tggcgaaagc
     2761 gttcaggtgg gcgggccccg gagggcgatc aaacttaaga aggtgccgat agtgaaggat
     2821 gccatactgc gatatgacgg gcgggagtat gccctgcccg tcgtcgaggg ctcggagggt
     2881 gaacgcgcgg ttgacattgc caacctccgg aacgattcgg gcctgatcac ccttgatgtc
     2941 ggctaccaga acacggggtc gtgcaagagc gccattacct tcatcgacgg cgatcgcggc
     3001 attctccgtt atcgcggcta tcccatcgag gagctcgccg agctctcgac gttcccggaa
     3061 accgcatttc ttctaattta cggtcatctg ccctcccgcg aggagcgcgc ggtgttctcg
     3121 cagcgtctca cggaaaactc gatgatccac gaggaaatgc tgcgcttttt cgacggcttt
     3181 ccgctcacgg cgcaccccat ggccatcctc tcgacaatgg tcaatgctct tgccgtgtac
     3241 tatccccgct attttaccga cgattgggag gtggcgacgt tcgatctcat ggcggcgcgc
     3301 ctcatctcga agattcggac catcgccgcc tattcgtaca agcattccat cggcgagccg
     3361 gtcgtctacc cgcgtcacga cttgaattat tgcgcgaact tcctctatat gatgttcaat
     3421 agcccggtca agccctacga tatggacgag gacatcgtga aggcgctcaa tgccctgctt
     3481 atcctgcatg ccgatcacga gcagaactgc agcacgtcca ccgtccgcct ggtgggcagc
     3541 agcatggtga acctctattc ctccatctgc gcaggcatct gcgccctgtg gggccctctc
     3601 catggcggcg cgaaccagca ggtcattgac atgctcgagg gcattcgcga ccaggggctc
     3661 accgtgcgga aatgcatcga gcaggccaag gacaagaaca gccccttccg cctcatgggc
     3721 ttcgggcacc gggtgtacaa gaattatgat ccgcgtgcgc gcatcttaaa agacaagctg
     3781 gacctgctcg tgcgcaaatt caacgtgaaa gagccgcttg tcgacatcgc gctcgagctc
     3841 gagcaggcgg ccctcacgga tgactacttc atcgagcgca agctctaccc gaacgtcgat
     3901 ttctacagcg gaatactcta ccgcatcatg ggcatcccgt cggacatgta cacggttctc
     3961 ttcgcgatcg gccggctccc cggctggatc gcccagtgga aagagatgca cgatacgctg
     4021 ccgtttcgaa tcggccggcc gcggcaggtc tataccggcg agaccctccg ttcctatgtg
     4081 ccaatcgacg cgcggtaggc cggttggcgc tatcggcgtt ttggtggata tcgaaattga
     4141 catttttggg cctatttgga agagtgcttc ttgtcgtgaa aactgttgat gtgttttcgc
     4201 tttacccatg aatcatcact gaatgcggga ctcgttggat tcgcgttccg cagcggcagg
     4261 tttaagcccc gggtgatcga cacggggatc tgttctgaac tgtgcgcgtg tctcatggcc
     4321 gacggtgggg tgtatcggat acccctttcc ggcggttgtg cggacagtcg aatttcaagg
     4381 gcgtaaaata ttcataacac agcgagggag tccgtatgcg ggaaattcgt tttcacggaa
     4441 gaggcggcca gggggcggtg acgtcggcgg agcttatcgc catttcggcg atcaacaagg
     4501 gcgggttcgc gcaggccttt ccgagctttg gcccagagcg gcggggcgcg ccggtcgtcg
     4561 cattctgcag ggtcgatgat aaaaagattc acatccgggc gaaggtatac aatcccgatg
     4621 tggtcgtggt gctcgatccc ggcctggtgg atcttctcga ccccactgaa ggcatgaaag
     4681 aaggcgacat gctcatcatc aatacgaaaa aaacggccga ccagatcaag ggactgaaga
     4741 acacctcgat aaaaattgcg acggtggacg cgaacaagat cgcgcaggaa gagcttgggc
     4801 gggtgattgt caacaccacc atgatcgggg ccttcataaa ggcgaccggg atcatatcta
     4861 ttgatgatat caccgaaccg atgatggagc gtttcggcgc gaagctcggc gagcgtaaca
     4921 tgaaggcgct caagcgcgca tatcaagaag tcacgataaa gggataatgc tatggcgaaa
     4981 ccgattgacg aaatcacctg gcaggaattg gggatcggtg cggttgtcac cgaaataggg
     5041 aattccgttc agtataaaac gggctcctgg cgttcggagc gcccggtggt caagaaggag
     5101 cattgcatca aatgcggcgt atgctggctg ttctgccccg acatgagcat cgtccaaacg
     5161 cccgaggggc attttacggc gaacctggag ttttgcaaag ggtgcgggat ctgcgcgaag
     5221 gaatgcccgg tgggatgcat cacgatgcaa ggtgaggagg cctgagatga gcgaaatagg
     5281 caaaggaaga aaagggatcg aggtgtccat cgcaatggcc gatgcggtgg cgcaggccga
     5341 tgtcgatgtc atcgccgcat atccgatcac tccccagacg cacatcgtcg aacatctggc
     5401 cgatatcgtg gccaacggcg agctcgatgc cgaatttgtc cacgtggaga gcgagcattc
     5461 cgcgatgagc gtgtgctgcg gttcggccgc ggtcggtgcg cgcacgttta cctcgaccag
     5521 ctcgcagggc ctggccttga tgagcgaaat attcattatc gccgcggcca tgcggctccc
     5581 gatggtgatg gcgctagcga accggtcgct ttccagcccg ctttcaatct ggaacgacca
     5641 taccgacgtg atgatggtgc gcgatgcggg ttggatacac ttcttcgttg aaaac
//
LOCUS       DAORHF010000243         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14521_length_5670_cov_5.453072, whole genome shotgun sequence.
ACCESSION   DAORHF010000243 DAORHF010000000
VERSION     DAORHF010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14521_length_5670_cov_5.453072"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PKJ16_15605"
     CDS             complement(<1..346)
                     /locus_tag="PKJ16_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HNV48468.1"
                     /translation="MIPALFRRSPQSCKPREECGVFGIFGNENASNLIYLGLYALQHR
                     GQESAGIATGDGHHIFRYAGMGKVVDIFKTRHIQNLQGFMGIGHNRYSTTGSSFLRNA
                     QPLRADSILGPIV"
     gene            complement(493..1059)
                     /locus_tag="PKJ16_15610"
     CDS             complement(493..1059)
                     /locus_tag="PKJ16_15610"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HNV48469.1"
                     /translation="MNSGDIRVGENIKEIRETMGLSLHDLAAKTGFSTALLSQIENHL
                     ISPPLGAIMNIARAMNIEIGRLFNQQHRAPFTIVRKNERQETSRVASKEGVRYGYAYE
                     SLAPEKVNRKMEPFLVTLEPASKKGVPYNHDGEEFLFVLEGRVEIQLGDFSDILEPGD
                     SIYYDSTVPHRVSCVDDVPAQIIAVIHA"
     gene            1233..1589
                     /locus_tag="PKJ16_15615"
     CDS             1233..1589
                     /locus_tag="PKJ16_15615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HNV48470.1"
                     /translation="MSNNSSARVEGYVTHEPQLRTTKTGKTICHFSVAMNHYSSPEES
                     PKVSFIDIETWEKLADFCGKSINKGRRVMVFGQLRQDRWEGKDGRIQSKIKVVGREIK
                     FLESSSVKTGDDASAN"
     gene            complement(1613..3094)
                     /gene="glgA"
                     /locus_tag="PKJ16_15620"
     CDS             complement(1613..3094)
                     /gene="glgA"
                     /locus_tag="PKJ16_15620"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HNV48471.1"
                     /translation="MKIAFVASEAYPFVKTGGLADVSYSLPRELARRGHDVRLFLPRY
                     YRIDKRNFRLALVHAPLGVPLGFGEKWAALYESPHAHPVRTYFIEHDAYFGRDGLYDD
                     GYSAYADNPERFTFFCRAVFQAMKAFEFRPDIIHCNDWQTGLVPVYRKTHYASDPDFA
                     GAATVMTVHNVGYQGVFHKDNLFWTQLGWDLFTVDGLEYYDQINFLKGGILLADAVTT
                     VSRRYAQEIQSEEFGYNLAPEFRKIRERLHGITNGVDYEKWNPATDPRIPKTYSASSI
                     AGKTECKLHLQHRMGITVDRGIPLMGIISRLTYQKGTDVLAESLFTAMRTDPFQFVVL
                     GSGDEGTIQKFEHLRATFPGRVGLFWGHDEDLAHLVEAGIDLFIMPSRYEPCGLNQMY
                     SLKYGSVPIVRATGGLDDTVVDWDEAHATGTGFKFSELTHESVCAAISRAIAVYWNAA
                     AWKKLQQNAMKFSYSWGDAVIEYERLYSSLAGPAKNRSADRRP"
     gene            complement(3091..4743)
                     /gene="gpmI"
                     /locus_tag="PKJ16_15625"
     CDS             complement(3091..4743)
                     /gene="gpmI"
                     /locus_tag="PKJ16_15625"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HNV48472.1"
                     /translation="MNPLKKIAAPVPRRGPLVFIIMDGVGIGPDNEGNALRLARTPNL
                     DRLHATCPVTSLLAHGPAVGLPSDDDMGNSEVGHNALGAGRIFSQGAKLVNAAIESGA
                     IFESDTWKQLVARPSSHGSTLHFMGLLSDGNVHSHIDQLFRMIAGAAAAGVSRMRAHI
                     LLDGRDVPGQSAEIYIDRLERELDAYRAKGLDYRIASGGGRMITTMDRYEADWSIVKR
                     GWDAHVLGEARPFRSAREALDTFRSEDPAIIDQYLPSFVIVDEAGPVGPILDGDSVIF
                     FNFRGDRAIEISRAFEEDAFTKFDRVRRPDALYAGMMEYDGDLKIPSRYLVSPPAIDR
                     TVSEYLARAGCRQFAISETQKFGHVTYFWNGNRSGKFDEATETYQEIPSDRVEFDQRP
                     WMKAADITDAVIAAVRSGSYDFIRLNYPNGDMVGHTGHLEAAIVAAETVDLSLGRLLP
                     VIAECGGIALITADHGNLDEMYQLDKKGAIQRDAQGVPVPRTAHTLNPVPFIVYDPGF
                     AGGYRLATTDGRPGLGNVAATILFFLGYEPPEGYLPSLVAPA"
     gene            complement(4853..5149)
                     /locus_tag="PKJ16_15630"
     CDS             complement(4853..5149)
                     /locus_tag="PKJ16_15630"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48473.1"
                     /translation="MTTYRTSETPTEVILTPQGDIVSILPGGLRERLHELIENDTRNI
                     VIDIAASTYIDSLSLAVLVDCKNKLAEKGRTIRLLNPNDDIATIISVASLDVLL"
     gene            complement(5160..>5670)
                     /locus_tag="PKJ16_15635"
     CDS             complement(5160..>5670)
                     /locus_tag="PKJ16_15635"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV48474.1"
                     /translation="SGIASGSDVILIPEIPFSLDIVCEKVVERSVSGKRYSIICVAEG
                     AKVSGQEVILEGKDKRTGVVRLGGIGNLISNEIETRTGIESRTSVLGHMQRGGSPTPY
                     DRVLATRYGSRAAALAMEGVFGVTVCLKGNEIDHFPLEKVANNPRRVPPDSEFVKIAR
                     TVSTSFGDY"
BASE COUNT         1003 a         1679 c         1905 g         1083 t
ORIGIN      
        1 gcacgatcgg gcccaggatg gagtcggccc gcagcggctg ggcgtttcgg agaaatgacg
       61 atccggtggt cgagtagcgg ttgtgcccga tccccatgaa cccctgcagg ttctggatgt
      121 gccgcgtctt gaagatgtcc accaccttgc ccattccggc gtagcggaag atgtgatgcc
      181 cgtcgccggt cgcgatgccg gccgattcct gcccgcggtg ctggagcgcg tagaggccca
      241 ggtagatgag gttcgatgcg ttttcgtttc cgaagatccc gaacacgccg cattcctcgc
      301 ggggcttgca ggactggggt gaacgccgga acagggctgg tatcatggat gcggtccgtt
      361 ggttcggcat gaacggctca gaattcgatg agaatcgaaa tggcgaaatc tgtaaaggaa
      421 attttttccg atccccgggg ggcgtcgctc gcgggggttt agcgcggcta aacgtccgtt
      481 cgccgtgctg tgtcatgcat ggatgacggc gatgatctgc gccggaacgt cgtccacgca
      541 cgaaacccgg tggggtacgg tcgaatcata atagatgctg tcgccgggct cgaggatgtc
      601 cgagaagtcg ccaagctgga tctccacgcg cccctcgagc acgaacaaaa attcctcgcc
      661 gtcgtggttg tagggaacgc ccttcttcga ggcgggttcc agcgttacca ggaacggctc
      721 cattttgcgg ttcaccttct ccggggcgag cgactcgtag gcgtacccgt agcgcacgcc
      781 ctctttcgac gccacgcgcg aggtttcctg gcgctcgttc ttccggacga tggtgaaggg
      841 cgcgcgatgt tgctggttga acaggcggcc gatctcgatg ttcatggcgc gcgcgatgtt
      901 catgatcgcg cccagcggcg gggaaatgag gtggttttct atctgggaga gcagcgccgt
      961 ggagaagccg gtcttggcgg caagatcatg gagcgaaagc cccatcgtct cgcgtatctc
     1021 cttgatgttt tcaccgacgc gaatgtcgcc gctgttcatg ggatgctcct ggccgctggg
     1081 gatgatgtga atatcatgat ggcacgccgg gccgatcgtg taaagagaaa tatttatgct
     1141 gaaatgcgcc atcaacacga aaaaaataga aaaatgccgc cggggcggac gtatctccag
     1201 tgtagacaac attttaccaa caggagtgag gtatgagcaa caattcgtca gcgagggtcg
     1261 agggatatgt tacgcacgag ccgcaattgc ggaccacgaa aaccggaaaa acgatctgcc
     1321 atttttccgt ggccatgaat cattattcct cgcccgagga gtccccgaag gtatcgttta
     1381 tcgatatcga aacctgggaa aagctcgcgg acttttgcgg gaagagcatc aacaagggca
     1441 ggcgggtgat ggtgtttggg cagctgcgac aggaccgctg ggagggcaag gacggccgca
     1501 tacagtctaa aatcaaggtg gtcgggcggg agatcaaatt tctggaatca tcatctgtga
     1561 agaccgggga tgatgcatct gccaattaac gctgtggtcc gcgggcggtc gcttacgggc
     1621 ggcggtccgc ggaccggttt ttcgccggtc ccgccagcga cgagtacagg cgttcgtatt
     1681 cgatcacggc gtcgccccat gaatagctga atttcatggc gttttgctga agtttcttcc
     1741 acgccgcggc gttccagtag actgcaatcg cgcgcgagat cgcggcgcac acggactcgt
     1801 gcgtgagctc ggagaacttg aagccggttc cggtcgcgtg cgcctcgtcc cagtccacga
     1861 ccgtgtcgtc cagcccgccc gtcgcgcgga cgatgggcac cgagccgtac ttgaggctgt
     1921 acatctggtt gagcccgcag ggctcatagc gcgacggcat gatgaacagg tcgattcccg
     1981 cctcgaccag gtgcgcgagg tcctcgtcgt gcccccagaa gagccccacg cgtccgggga
     2041 aggtcgcccg caggtgctcg aacttctgga tcgtcccctc atcgccggag cccaggacca
     2101 caaactggaa ggggtcggtt cgcatcgccg tgaacagcga ctccgcaagg acgtcggtcc
     2161 ccttctggta ggtgagtcgc gagatgatgc ccataagcgg tatcccgcgg tcgacggtga
     2221 ttcccatccg gtgctgcagg tggagcttgc attccgtttt gccggcgatg gaggatgcgg
     2281 aataggtctt gggtatgcgc gggtcggtcg cggggttcca tttttcgtaa tcgacgccgt
     2341 tggtgatgcc gtggaggcgc tcgcggatct tgcggaactc gggcgcgaga ttgtaaccga
     2401 attcctcgct ctgtatctcc tgcgcatagc gccggcttac ggtggtgacg gcgtcggcca
     2461 gaaggatccc tcccttgagg aagttgatct ggtcgtagta ttccaatccg tccacggtga
     2521 acaggtccca gccgagctgc gtccagaaga ggttgtcctt gtggaacacg ccctgatagc
     2581 ccacgttgtg gacggtcatg acggtcgccg cgccggcgaa atccgggtca ctcgcgtagt
     2641 gcgttttccg gtacaccgga acgagccccg tctgccagtc gttgcaatgg atgatgtccg
     2701 ggcgaaactc gaacgccttc atcgcctgaa aaaccgcccg gcaaaaaaac gtgaagcgct
     2761 ccgggttgtc ggcgtaggcg ctgtagccgt cgtcgtagag cccgtcccgg ccgaagtagg
     2821 cgtcgtgctc gatgaaatag gtccgcacgg ggtgggcgtg gggtgattcg tacaacgccg
     2881 cccatttctc tccgaagccc agcggcacgc cgagcggcgc gtggacgagc gcgaggcgga
     2941 agttccgctt gtcgatccgg taatagcggg gaaggaacag ccgcacgtcg tggccccgcc
     3001 gcgcgagctc ccgcggcagc gagtacgaga cgtcggccag tccgccggtt ttcacgaacg
     3061 ggtaggcctc ggatgcgaca aaggcgatct tcatgccggg gccacgaggg acggcagata
     3121 gccctccggc ggctcgtacc cgaggaagaa caggatcgtg gcggcgacat tgcccaggcc
     3181 gggacggccg tccgtggtcg cgaggcggta gccgccggcg aagccgggat catacacgat
     3241 gaaggggacc ggattgaggg tgtgcgccgt gcgcggcacg gggaccccct gcgcgtcccg
     3301 ttggatggcg cccttcttgt cgagctggta catctcgtcg aggttgccgt ggtcggcggt
     3361 gatgagcgcg atcccgccgc actcggcgat caccgggagc aggcggccca gcgagaggtc
     3421 gaccgtctcg gccgcgacga tggcggcctc gagatggccc gtatggccga ccatgtcgcc
     3481 gttggggtaa ttgagcctga tgaaatcgta cgatcccgag cgaacggccg cgatcaccgc
     3541 atcggtgatg tccgccgcct tcatccacgg gcgctggtcg aactccacgc ggtcggaggg
     3601 gatctcctgg taggtctcgg tggcctcgtc gaacttgccg ctgcggtttc cgttccagaa
     3661 gtaggtcacg tggccgaact tctgcgtctc ggagatcgcg aactgacggc agcccgcgcg
     3721 cgcgaggtac tcgctcaccg tgcggtcgat cgccggcggc gaaaccagat agcgcgacgg
     3781 gatcttgaga tcgccgtcgt attccatcat gccggcgtag agcgcgtcgg ggcgccgcac
     3841 gcggtcgaac ttggtgaagg cgtcctcctc gaaggcccgc gagatctcga tggcgcgatc
     3901 gccgcgaaag ttgaagaaga tcacagaatc gccgtcaagg atcggtccca ccgggccggc
     3961 ctcgtcgacg atcacgaacg acgggagata ctggtcgatg atcgccgggt cctcgctgcg
     4021 gaaggtgtcg agcgcctcgc gcgccgaccg gaacggacgc gcctcgccca gcacgtgcgc
     4081 gtcccagccg cgctttacga tgctccagtc ggcctcgtag cggtccatgg tggtgatcat
     4141 ccggcctccg cccgaggcga tgcgatagtc gagccccttc gcgcggtagg cgtcgagctc
     4201 gcgttcaagg cggtcgatgt atatctccgc cgactggccc ggcacgtcgc gcccgtcgag
     4261 caaaatatgc gcgcgcatgc gtgatacgcc cgccgcggcg gcgcccgcga tcatgcgaaa
     4321 gagctggtcg atatgcgagt gcacgttgcc gtccgagagg agccccatga agtgcagcgt
     4381 cgagccgtgc gacgaggggc gtgcgacgag ctgcttccag gtatcgcttt cgaagatggc
     4441 gcccgattcg atcgccgcgt tcaccagctt ggcgccctgg gagaagatgc gcccggcgcc
     4501 gagcgcgttg tgtcccacct ctgagttccc catgtcgtcg tcgctgggga gccccaccgc
     4561 ggggccgtgc gccaagagcg acgtgacggg acaggtcgca tggaggcggt ccaggttcgg
     4621 cgttcgcgcg aggcgaaggg cgttgccctc attgtcgggg ccgatcccca cgccgtccat
     4681 gatgatgaag accagcggtc cgcggcgcgg gaccggtgcg gcgatttttt tcagtggatt
     4741 catcgggatt tcctcgggtc gatagtcggc gatcaccggc atgcgccggt gcggcaactc
     4801 catcagcgat ggccgtattt ttcaagtact ttgcgcggcg gcggaacggg cgctacagga
     4861 ggacgtccag ggaggcgacg ctgatgatcg tggcgatgtc gtcgttcggg ttgagcagac
     4921 ggatcgtgcg ccccttctcg gccagcttgt ttttgcagtc cacgaggacg gccagcgaca
     4981 gcgagtcgat gtacgtcgat gcggcgatgt cgatcacgat attgcgcgtg tcgttctcga
     5041 taagctcgtg cagccgctcc cgcaggccgc cgggcagaat cgacacgatg tcgccctgcg
     5101 gcgtgaggat tacctcggtg ggcgtttcgg atgtgcgata ggtggtcatc gcagcacgct
     5161 cagtagtccc cgaaggatgt gctcacggtg cgcgcgatct tcacgaactc ggagtccggg
     5221 gggacgcgcc gcggattgtt cgcgaccttc tcgagcggga agtggtcgat ctcgtttcct
     5281 ttgaggcaaa cggtaacgcc aaagacgccc tccatggcga gcgccgcggc gcgagaaccg
     5341 tagcgcgtcg cgagcacgcg gtcgtagggt gtgggcgatc cgccgcgctg catgtgtccc
     5401 aggaccgagg tgcgcgactc gatgccggtc cgcgtttcga tctcgttgga tatcaggttt
     5461 ccgatgccgc cgagccggac cacgcccgtt cgcttgtcct tgccctcgag gatcacctcc
     5521 tgtcccgaga ccttcgcgcc ctccgccacg cagatgatgc tgtagcgctt gccggagacg
     5581 ctgcgctcca ccaccttctc gcagacgata tcgaggctaa acgggatctc gggaatgagg
     5641 atcacgtcgc tgccgctcgc gatccccgag
//
LOCUS       DAORHF010000244         5608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14743_length_5608_cov_4.646677, whole genome shotgun sequence.
ACCESSION   DAORHF010000244 DAORHF010000000
VERSION     DAORHF010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14743_length_5608_cov_4.646677"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PKJ16_15640"
     CDS             complement(<1..998)
                     /locus_tag="PKJ16_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48475.1"
                     /translation="MSDNIRTITDRTLFEKIFYKFFMGNPVFIKTKSGNLKIRFFGYS
                     EGLVALNISLLKNAPDTCLIFTKHQDYLVYAHLKFAEKQEETIYLFHALKMQIITATR
                     REDRVALVDADGEGGKRIVYLSNVISDFIIENSLAMTQKKVDHIKEMMRYDLQKQFEY
                     LKIFFINEGTTDPRMKQFIEDVTPIYIANLNEKPKEKYEKAFNSYINEIYSRDYYLRN
                     RKNFISEITVPILYRMKVPYGYIQVNGSNPFPESALTVIKRMAIVIEELFTKDKIFPV
                     SDERLLVSDVSKRGLGVAFRERRFIRYFKEKSMVYFDLFLPDGKRASILGVVRNISL"
     gene            1123..1974
                     /locus_tag="PKJ16_15645"
     CDS             1123..1974
                     /locus_tag="PKJ16_15645"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HNV48476.1"
                     /translation="MAVQRHGRRTIAGSLRAGYIFSILPDSVKSFLLGRLSPREAGRL
                     DRALGRIGGLTNADRMEALGSFTHGMRRLREEREAAVDRSLVATLALVAGAIALAAVY
                     SIAMAKNPDGAFAIIDFLVTHGGMHLILLPCLWTVIRVEYRTAPLRALFGSQNIALDI
                     GIGIVAGIALGALFVQPGPRVHSTLPAGARFAAGMLAVTVGPAVEELFFRFILFYRVG
                     RRHGMAAGAALSVCAFVAAHLPHTLPLAAAYLVAGGALCALYAGRRSLLPALTAHAVS
                     NAAVLLL"
     gene            complement(1971..3491)
                     /locus_tag="PKJ16_15650"
     CDS             complement(1971..3491)
                     /locus_tag="PKJ16_15650"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HNV48477.1"
                     /translation="MIYTFSDYSGPIDERCDVCVIGSGAGGAVAAMELAESGLSVIVL
                     EEGGLFTPKSWTGKPTQALIDMYRDGGSTGALGTPFISTTLGKCVGGTTTVNSATCFR
                     TPEVVLQRWQMELGLRDLTMERLAPVFERVERIINVTELSWDVLGNSAKIIKRGADRL
                     GLACRPLKHNVKDCRGCGTCQFGCVENAKQSMDVTYIPRAIACGARLFANCRADRLVI
                     EKGAVRGVVGSVVDPHSGERVFDVTIASRAVVAACGALITPALLKRSGLRNRNIGRHL
                     QIHPASRVVALMNETVEGWKGVSQGVYVHNYETEGIILEGIFIHPSLMLAALPGVGPE
                     HKELAARYANLAAFGVMVHDSTVGRVRRGISRNGFLATYFITRADAEKLKRGIAYAAR
                     VFFAAGAERVYAPVASMPVLSSADDVEKLLGLPVRPNQIETMAFHPLGTCRMAESPRL
                     GAINEKGESFEVRDLYVADAGAVPTSLGVNPQVTIMALATIVAQGIARRFSGRPSS"
     gene            complement(3488..3961)
                     /locus_tag="PKJ16_15655"
     CDS             complement(3488..3961)
                     /locus_tag="PKJ16_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48478.1"
                     /translation="MPHRVLTSTTHRAIMNAVMRAIVPRGGPFAAGAADYDLIPRVRE
                     ILLSYHPIIARGFGLVLWYVHLSALPRYGRPFASLPEETATAFLASMEHSPLYYRRSA
                     VLLLKLLTFLALYDIDEVARAIGYAPDCGPDRPGRESKRPARRAARTTAKRKTRT"
     gene            complement(3986..>5608)
                     /locus_tag="PKJ16_15660"
     CDS             complement(3986..>5608)
                     /locus_tag="PKJ16_15660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48479.1"
                     /translation="DGSQAMKIEFVGGARTVTGSSYILTTDRCTIMIDCGMFQGNAAL
                     RDRNALGLIYAPKKIDALLLTHAHIDHSGLIPKLVKEGFENKIFATSATVDLCAIMLP
                     DSAHIQESDAEWRTRKQRKRGEPGVEPLYTMADAEKAVQFFVPVRYDDLVDIAPGVRA
                     RFRDDGHILGSAFIELMVEDGGTTRTIVFSGDIGSSDQAIIRDPELPDNADVLFIEST
                     YGDRLHKSKKDTYEEFKNIILESHNRKGNIIIPSFAVERTQEIIYTLGKLFAAGDIPR
                     IPVYIDSPLAINATEVFLRNTAYFDDEMKQLLASGVNPLEFPNLTFVRTTEESIALNK
                     DAKGSIIISASGMCTAGRIKFHLANNLYRPESSVIFVGFQAEGTLGRMLVDGATRVKI
                     YGEDVVVKSRIYTLGGFSGHADRDGLLAWMSRIKNEKLTVFVIHGEERSSLALADSIK
                     ERFGYTTYVPRWGEIVDIDTLQSSMAQYGTHAAPTAPPLEGDIERVLGSITGALDRYR
                     EAVRNNRADEMRKFESRLAELKAVLQSLEEDQ"
BASE COUNT          946 a         1800 c         1781 g         1081 t
ORIGIN      
        1 agcgatatgt ttcgcaccac gccaaggatc gacgcccgct tgccgtccgg caggaacagg
       61 tcgaagtaga ccatgctttt ttccttgaaa tagcggatga agcgccgctc gcgaaaggcc
      121 acaccgaggc cgcgcttcga cacgtccgac accagcagac gctcgtcgga caccggaaaa
      181 atcttgtcct tggtgaacag ctcctcgatg acgatcgcca tgcgcttgat gaccgtgagc
      241 gccgactccg gaaacgggtt cgacccgttc acctggatgt agccgtaggg gaccttcatc
      301 cggtagagga tggggaccgt gatctcggag atgaagttct tccggttgcg aagatagtaa
      361 tcgcgcgaat atatctcgtt tatatacgaa ttgaaggcct tttcgtattt ctccttcggc
      421 ttctcgttga gattggcgat atagatgggc gttacgtcct cgatgaattg cttcatgcgc
      481 gggtcggtgg tcccctcgtt gatgaaaaaa atcttgaggt attcgaactg cttctgcagg
      541 tcgtagcgca tcatctcctt gatgtggtcc accttcttct gcgtcatggc cagcgaattt
      601 tcgatgatga agtcggagat cacgttgctg aggtatacga tgcgcttgcc gccctcgccg
      661 tccgcatcga ccagcgccac gcggtcctcg cggcgcgtcg cggtgatgat ctgcatcttg
      721 agcgcgtgaa agagatagat ggtctcctcc tgcttctccg cgaacttgag gtgcgcatac
      781 accagatagt cctggtgttt ggtgaagatg agacaggtgt ccggcgcatt tttcaggagc
      841 gagatgttga gcgccaccaa cccctcggag tagccgaaaa agcgtatctt cagattcccg
      901 cttttcgtct tgatgaagac gggattgccc atgaagaact tgtagaagat cttctcgaag
      961 agcgttctgt cggttatggt tcgtatattg tcggacattc gtttcgttcc ggaccgcgcc
     1021 gccggcaccc tcgggccagt gcgcggaaaa aacgcttgtt acgatgcggc gcctcgtgca
     1081 tggtatcgct cggtcgcgca acatcgacgc atgcgggtca gtatggcggt acaacgacac
     1141 ggccggagaa cgatcgcggg atcgctccgg gccggttata tcttctcgat tcttcccgat
     1201 tctgtcaagt cattccttct cggacgattg tcgccgaggg aggccgggcg gctcgaccgc
     1261 gcccttggcc gcatcggcgg cctcacgaac gccgatcgca tggaggcgct cggctcgttc
     1321 actcacggca tgcgacgcct gcgcgaggag cgcgaggccg ccgtggaccg ctcgctcgtc
     1381 gcgacactcg cgctggtcgc gggcgcgatc gcactcgccg ccgtctattc aatcgcgatg
     1441 gcaaaaaatc ccgatggcgc ctttgccatc atcgacttcc tggtgacaca cggcggcatg
     1501 catctcatcc tcctcccctg cctgtggacg gtgatccgcg tggaataccg tacggcgccg
     1561 cttcgcgcgc tgttcggatc gcaaaacatc gcactcgaca tcgggatcgg gattgtcgcc
     1621 ggaatcgcgc tcggcgcgct ctttgtccag cccgggcccc gcgtccactc cacactgccc
     1681 gccggcgcgc ggttcgccgc cggcatgctc gccgtcaccg tgggtccggc ggtcgaggag
     1741 ttgttttttc gattcatcct gttttatcgg gtgggacgcc ggcacggcat ggccgcgggc
     1801 gcggcgctct cggtgtgcgc attcgtcgcg gcgcatcttc ctcacacgct cccgctggcg
     1861 gcggcatatc tggtcgcggg gggcgctctc tgcgcgctgt atgccggccg acggtcgctg
     1921 ctccccgccc tcacggcgca tgctgtatcg aatgccgcgg tgctgcttct ttaagacgac
     1981 gggcggcccg agaagcgccg tgcgatgccc tgcgcgacga tcgtcgcgag cgccatgatg
     2041 gtcacctgcg gattcacgcc aagcgaggtg gggaccgcgc ccgcatcggc gacgtagaga
     2101 tcgcgcacct cgaagctctc gcccttttcg tttatcgcgc cgagccgcgg ggactccgcc
     2161 atgcggcagg tcccgagggg atgaaacgcc atggtctcga tctggttcgg gcgcacgggg
     2221 agcccgagaa gtttttcgac atcgtcggcg gaggacagga ccggcatgga cgctacgggc
     2281 gcgtacaccc gctcggcgcc ggccgcgaag aacacgcgcg cagcataggc gatcccgcgc
     2341 ttcagcttct cggcgtcggc gcgggtgatg aagtaggtcg ccagaaaacc gttgcgcgaa
     2401 attccgcgcc gcacccggcc cacggtgctg tcgtgcacca tcaccccgaa ggccgcgagg
     2461 ttcgcatacc gcgcggcgag ctccttgtgc tccggcccca cgcccggcag cgccgcgagc
     2521 atgagcgacg ggtggatgaa gatcccctcc aggatgatcc cctcggtctc gtaattatgg
     2581 acatagacgc cctgcgaaac ccccttccat ccctcgaccg tctcgttcat cagcgccacc
     2641 acgcggctgg ccgggtggat ctggaggtgc cgcccgatgt tccggttgcg aagcccggag
     2701 cgcttcagga gcgccggggt gatgagcgcg ccgcacgccg ccaccaccgc gcgcgaggcg
     2761 attgtcacgt cgaacacccg ctcgccgctg tgcggatcga cgacggaccc cacgacgccg
     2821 cgcaccgccc ccttttcgat gaccagccgg tcggctcgac aattcgcgaa aaggcgcgcc
     2881 ccgcatgcga tcgcgcgcgg gatgtaggtc acgtccatgc tctgcttggc gttctccacg
     2941 cagccgaact ggcaggtgcc gcacccgcgg cagtccttca cattgtgctt gagcggccgg
     3001 caggcgaggc ccagacggtc cgcgccgcgc ttgatgattt tcgccgagtt ccccaggacg
     3061 tcccacgaga gctccgtgac attgatgatc cgctcgacgc gctcgaacac cggggcgagg
     3121 cgctccatgg tgagatccct gaggccaagc tccatctgcc agcgctgcag gacgacctcg
     3181 ggcgtccgga aacaggtggc ggaattcacg gtcgtggtgc cgcccacgca tttgcccagc
     3241 gtggtggaga tgaagggggt tcccagcgcg ccggtggagc cgccgtcgcg atacatgtcg
     3301 atgagcgcct gcgtgggctt gcccgtccac gatttcggcg taaacaggcc gccctcctcg
     3361 agcacgatca cggacagccc gctctcggca agctccatcg cggccaccgc gccgccggcg
     3421 ccgctgccga tcacgcacac gtcgcagcgc tcgtcgatgg gtccggaata atcgctgaat
     3481 gtgtatatca tgtccgtgtc ttccgcttcg ccgtggtccg ggccgcccga cgagcggggc
     3541 gtttcgattc gcgcccggga cgatccggcc cgcagtccgg cgcatagccg atcgcgcgcg
     3601 cgacctcatc aatgtcgtat aacgcgagaa aggtaagcag cttgagcaac agcaccgcgg
     3661 agcgccggta gtacagggga ctgtgctcca tcgacgccag aaatgcggtc gccgtttcct
     3721 cgggaagcga tgcgaacggg cgcccgtagc gcggcagcgc gctcaggtga acataccaaa
     3781 ggacaagccc gaagccccgg gcgatgatgg gatgatacga cagcagtatc tcgcgaacgc
     3841 gcgggatgag atcgtaatcg gccgctcccg cggcgaaggg accgccgcgc gggacgatgg
     3901 cgcgcatcac ggcgttcatg atggcccgat gggttgtgct cgtcaacact cgatggggca
     3961 tgcggcgctc cttccgcgcg ggcggctact ggtcctcttc gagggactgg aggacggcct
     4021 tgagctccgc caggcggctc tcgaacttcc gcatctcgtc ggcgcgattg ttccgcaccg
     4081 cctcgcgata acgatcgagc gcgccggtta tcgatcccag cacccgttcg atatcgccct
     4141 caagcggcgg cgcggtgggc gccgcgtgcg tgccgtactg cgccatcgag ctctgcaggg
     4201 tgtcgatatc gacgatctcc ccccagcggg ggacataggt cgtatagcca aagcgctcct
     4261 tgatggaatc ggcaagcgcg agcgatgacc gctcctcgcc atggatcacg aacacggtga
     4321 gcttctcgtt cttgatgcgg ctcatccacg ccaggaggcc gtcgcgatcg gcatgccccg
     4381 agaacccgcc cagggtgtag atgcgcgatt tcaccaccac gtcctcgccg tagatcttca
     4441 cgcgcgttgc gccgtccacc agcatgcggc ccagcgtccc ctccgcctgg aaccccacga
     4501 agattacgct cgactccggc cggtagaggt tgttggccag atggaacttg atccgccccg
     4561 cggtgcacat cccgctggcc gagatgatga tggagccctt cgcgtccttg ttgagcgcga
     4621 tcgattcctc ggtcgtgcgg acgaacgtca ggttgggaaa ctcaagcggg ttcacgcccg
     4681 aggccagaag ctgcttcatc tcgtcatcga aatatgcggt gttccggagg aacacctcgg
     4741 tcgcattgat cgccaggggc gaatcgatgt acacgggaat gcgcggaatg tccccggccg
     4801 caaagagctt gcccagggtg taaatgatct cctgcgtgcg ctccaccgcg aaggagggga
     4861 tgatgatgtt ccccttgcgg ttgtgcgact cgaggatgat gttcttgaac tcctcgtagg
     4921 tgtccttctt gctcttgtgc agccggtcgc cgtaggtcga ttcgatgaag agcacgtcgg
     4981 cattgtccgg gagctcggga tcgcggatga tcgcctggtc gctggagccg atgtcgccgg
     5041 agaagacgat ggtccgcgtt gtcccgccgt cctccaccat gagctcgatg aaggcggagc
     5101 cgaggatgtg gccgtcgtcg cggaagcgcg cccggacccc gggcgcgata tcgacgagat
     5161 cgtcgtagcg caccgggacg aagaactgga ccgccttctc ggcgtcggcc atcgtataca
     5221 gcggctcgac gcccggctcg ccgcgcttgc gctgcttccg cgtgcgccac tcggcgtcgc
     5281 tctcctggat gtgcgcgctg tcgggcagca tgatggcgca gaggtccacc gtcgcggagg
     5341 tcgcgaagat cttgttctcg aagccctcct tgacgagctt ggggatgagc ccgctatggt
     5401 cgatgtgcgc gtgcgtgagc agcagcgcgt cgattttctt gggcgcgtag atgagcccca
     5461 gcgcattgcg gtcacgcagc gccgcgttcc cctggaacat cccgcagtcg atcatgatgg
     5521 tgcaccggtc ggtggtgagg atgtacgacg acccggtgac cgttcgcgcg cccccgacga
     5581 attctatctt catggcttga ctcccatc
//
LOCUS       DAORHF010000245         5603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_14766_length_5603_cov_4.323901, whole genome shotgun sequence.
ACCESSION   DAORHF010000245 DAORHF010000000
VERSION     DAORHF010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14766_length_5603_cov_4.323901"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="PKJ16_15665"
     CDS             complement(<1..172)
                     /locus_tag="PKJ16_15665"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HNV48480.1"
                     /translation="MSRRKVAIIGAGSWGTTIAKVIAENHPDVPIVMWAYEKNLPRAI
                     NERHENTQYLPGV"
     gene            complement(331..2085)
                     /gene="rpoD"
                     /locus_tag="PKJ16_15670"
     CDS             complement(331..2085)
                     /gene="rpoD"
                     /locus_tag="PKJ16_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HNV48481.1"
                     /translation="MSKGELVLNEIPEVKGLIELGKSNGELTYDEINDKLPEKILNSD
                     KIDDLFILLNQLGIEIVEESTRKTPVVKEKKKGKSKTKKAAAGATIDDPIRLYLKEIG
                     TVSLLSGDQEVDLAKKIEEGELLIEDAVVNSTLLVNDLVKHYPKIKSGKMKLVEILKI
                     NKLYYFSSADMHKLEKKFYERMVVILSDDKKIMQLESKLRKLEEGSPKGAELKKKIEE
                     HRASITEAVKDIEISESEIKKVAAKILSMVHRIQETQGFFTEVERKYKKSLKELKHYG
                     RKVEKGEQVKQTLAALKITGKDTLLNLVKDIRNNERKIRRIEQEAGAETEYILKWGKQ
                     IGLGQRKISIAKKELINANLRLVVSIAKKYANRGMHFFDLIQEGNIGLIKAVDKFEYR
                     KGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQINKVMRETRLFQQEFGREPTP
                     EELADRLGWPVSKIKGVKNVAREPISLETPVGEEEDSLLGDFIEDKYVDSPANTAAYR
                     LLHEQINTVLKTLPAREQKVIRMRFGLDDGYSHTLEEVGYVFKVTRERIRQIEAKALR
                     RLRHPTRARKLKDYLDYM"
     gene            complement(2116..3900)
                     /gene="dnaG"
                     /locus_tag="PKJ16_15675"
     CDS             complement(2116..3900)
                     /gene="dnaG"
                     /locus_tag="PKJ16_15675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HNV48482.1"
                     /translation="MQIPREIINRIRDRADIESVVRRYVPSLAKKGKDFIGLCPFHKE
                     KTPSFTVSVEKRIFYCFGCHAGGNVFSFISRIEGLSFPQSVRFVGDLVGIPVTDSANG
                     ASASPEDAYLKLNSVALGVFYNTLREPIGKPGLEYVLGRGVSREAIDEFKLGFAPESW
                     NHLTGFLKGNRANLEQCAEIGLVSKADKEGRIHYYDKFRNRLIFPICNASGEPIAFGG
                     RIIGPGEPKYLNSPESAVFQKRGVLYGFNRARDAIRELKRAIVVEGYLDVIGCHQQGV
                     RNVVAPLGTALTQQHVDLLARYCNEIILVFDADPAGINASLRSIDVISAKNVEVRVAA
                     LPESDPFEFVRTRGIREFMAAVDSALSPVDYRMRRVMAEALPGAANRTRTMVQLFEVI
                     RELPYESERSAYVKRISALLSLDENAVRVDFKKFLGRSEAPFAPSAGGRAERADFETK
                     SYRDLVALLCAHPRLVAKAVFDFSAEEIVDPAARSVFARLLDLYSADEEIYVDKIFDF
                     FSEGDEKRFLEENVAARSPVENPDDAYTEIYINLKLHQIDRKINRCADTIRRSGGADS
                     ADVRACLTEIEVLRREKEKLSLYVYNRR"
     gene            complement(3921..4370)
                     /locus_tag="PKJ16_15680"
     CDS             complement(3921..4370)
                     /locus_tag="PKJ16_15680"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HNV48483.1"
                     /translation="MSLLDTISGDLHEAIKAKNEDVVRTLRMLKSDIMYEKTKGTAEL
                     TDEAVLEVVARASKKRKEAIEEFTKAGRGELAAKEAAELEILARYLPEQLGEEAIAAA
                     IDRKIEEMGAVTAKDFGKIMSVIMKELKGRADGAIVKSLLGKKLEGR"
     gene            4792..>5603
                     /locus_tag="PKJ16_15685"
     CDS             4792..>5603
                     /locus_tag="PKJ16_15685"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV48484.1"
                     /translation="MKKAGIALSVLLLALCFVLFLSFDKIGSIWSQNVKKQSSARARE
                     AAKVAPAVPGFPSFYKGIYLTSNSGKDFKKLQKYVSMAQEGRLNTLVIDVQDSRIKES
                     AIPKQNIDYCIEKGFHTVARVVVFPDGLKKYPVDPQYIANKLAVAENACKLGFREIQF
                     DYIRFNDHGILKKIPLKEKYAFIENFLRKARAHLKPYNVRIAADVFGRIPLNKHDPIG
                     QRMEGLDEVADVICPMAYPSHYTWSKKLMSDPYTTVFITSKKAKERVTRAHIV"
BASE COUNT         1074 a         1726 c         1564 g         1239 t
ORIGIN      
        1 cgacccccgg caggtactgc gtgttctcgt gccgctcgtt gatggcgcgc ggaaggtttt
       61 tctcgtaggc ccacatcacg atgggcacat ccggatggtt ctccgcgatg accttcgcga
      121 tggtggtgcc ccacgatccg gcgccgatga ttgcgacctt tcggcggctc atcggcggga
      181 ccgcagcggc gcgatgtgtg atttcgtgag tgtgcacata cctggaagcc ccatcagaac
      241 ggaccgcggg gatggggtga tgatgccgaa ccggcgcgat aacgcaagca cattatcgaa
      301 aagcggacgc cgtcggcggg cgcgtcgcgc ctacatgtag tccaggtaat ccttgagctt
      361 gcgcgcgcgg gtgggatgcc gcaggcgccg cagggccttg gcctcgatct gccggatgcg
      421 ctcgcgcgtg accttgaaga cgtagcccac ttcctccaac gtgtgggaat agccgtcgtc
      481 cagaccgaag cgcatccgga tcaccttctg ctcgcgcgcg ggcaacgtct tgaggaccgt
      541 gttgatctgc tcgtgcagca ggcggtaggc cgcggtgttc gcgggcgaat cgacgtactt
      601 gtcctcgatg aaatcgccca ggagggagtc ctcctcctcg cccaccggcg tctccagcga
      661 gatgggctcg cgcgcaacgt tcttgacgcc cttgatcttg ctcaccggcc agccgagccg
      721 gtcggccagc tcctcggggg tgggctcgcg gccgaactcc tgctggaaca ggcgcgtctc
      781 gcgcatcacc ttgttgatct gctcgatcat gtgcacgggc acgcggatgg tgcgcgcctg
      841 gtcggagatg gcgcgggtga tcgcctggcg gatccaccag gtcgcgtagg tggagaactt
      901 gtatcccttg cggtactcga acttgtccac cgccttgatg aggccgatgt ttccctcctg
      961 gatgaggtcg aagaagtgca tgccgcggtt ggcgtacttc ttcgcgatgg agacaaccag
     1021 gcgcaggttc gcgttgatga gctccttctt cgcgatggag atcttccgct gccccagtcc
     1081 gatctgcttg ccccacttga ggatgtattc cgtctccgcg ccggcctcct gctcgatccg
     1141 gcggatcttg cgctcgttgt tgcgtatgtc cttcaccagg ttcagcagcg tgtccttgcc
     1201 cgttatcttc agggccgcga gcgtctgctt cacctgctcg cccttctcca ccttgcgccc
     1261 gtagtgtttg agctccttga ggctcttctt gtacttccgc tccacctcgg tgaaaaagcc
     1321 ctgggtctcc tggatgcggt gcaccatgga gaggatcttc gcggcgacct tcttgatctc
     1381 gctctcgctg atctcgatat ccttgaccgc ctcggtgatg ctggcgcggt gctcctcgat
     1441 cttctttttc agctcggcgc ccttgggcga tccctcctcc agcttgcgga gcttgctctc
     1501 cagttgcatg atcttcttgt cgtcggagag gatcaccacc atgcgctcgt agaacttctt
     1561 ctcgagcttg tgcatgtccg ccgacgagaa gtagtacagc ttgttgatct tgaggatctc
     1621 gacgagcttc atcttgccgc tcttgatctt cggatagtgc ttcacgagat cgttcaccag
     1681 cagggttgag ttgaccaccg cgtcctcgat gagcagctcg ccctcctcga tcttcttggc
     1741 caggtccacc tcctggtcgc cggagaggag cgacacggtg ccgatctcct tcaggtagag
     1801 gcggatcggg tcgtcgatgg tggcgccggc ggcggccttt ttcgttttag acttgccctt
     1861 tttcttttcc ttgacgaccg gcgttttgcg ggtcgattcc tcgacgatct cgatgccgag
     1921 ctggttgagg aggatgaaga gatcgtcgat cttgtccgag ttgaggatct tctcgggaag
     1981 cttgtcgtta atctcgtcgt aggtgagctc gccgttcgac tttcccagct cgatcaggcc
     2041 cttcacctcg ggtatttcgt tcaacaccag ctcgcctttg ctcataatga tggcacccct
     2101 cgcctgtgat atgccctagc gcctgttgta gacgtagagg gaaagctttt ccttttcgcg
     2161 gcgcagcacc tcgatctccg tgaggcacgc gcgcacatcg gccgaatccg ccccgccgga
     2221 ccgccgaatc gtgtcggcgc accggttgat tttccggtcg atctgatgca gcttgagatt
     2281 tatgtagatt tcggtatacg catcgtcggg attttcaacg ggagatctgg cagcgacgtt
     2341 ctcttccaga aaccgcttct cgtcaccttc agaaaaaaaa tcgaatattt tgtcaacata
     2401 tatttcttcg tccgctgaat acaggtccag caggcgggcg aagacggagc gcgccgcggg
     2461 atcgacgatc tcctccgcgg agaaatcgaa taccgccttc gcgacaagcc tcgggtgggc
     2521 gcacaaaagc gccaccaggt cgcgatagct tttcgtctcg aagtccgcgc gctcggcgcg
     2581 tcccccggca gagggcgcaa agggcgcttc gcttcgccca aggaatttct tgaaatccac
     2641 gcgtacggcg ttctcatcga gcgacagcag ggcgctgatc cgcttcacgt aggcgctccg
     2701 ctcggactcg tacggcagtt cgcggatgac ctcgaagagc tggaccattg tccgcgtccg
     2761 gttcgccgcc cccggaagcg cctccgccat cacgcgccgc atccggtagt ccaccggcga
     2821 gagcgcggaa tccaccgcgg ccatgaactc gcggatgccg cgcgtgcgga cgaactcgaa
     2881 cgggtcggac tcgggcaggg ccgccacgcg cacctcgacg ttcttggccg agatgacgtc
     2941 gatcgatcga agcgacgcat tgatccccgc gggatcggcg tcgaagacca ggatgatctc
     3001 gttgcagtag cgcgcgagaa ggtccacatg ctgctgcgtg agggccgtgc cgagcggtgc
     3061 gaccacgttc cgcaccccct gctgatggca gccgatcaca tccaaatacc cctccaccac
     3121 gattgcccgc ttgagttccc gtatggcgtc ccgggcccgg ttgaagccat agagcactcc
     3181 ccgcttctga aagacggccg attccgggga gttcaaatac ttcggttcgc ccggtccgat
     3241 gatacgcccc ccgaaagcga tgggctcgcc gctcgcgtta caaataggaa agatgagccg
     3301 gttcctgaac ttgtcgtaat aatggatccg gccctcttta tcggccttgc tcaccaggcc
     3361 gatctccgcg cactgctcca aattggcgcg gttgcccttg agaaatccgg ttagatgatt
     3421 ccagctctcc ggcgcgaatc ccagcttgaa ctcgtcaatg gcctcccgcg acacgccccg
     3481 gcccagcaca tactccaatc ccggtttgcc gatgggctcg cgcagcgtat tgtaaaaaac
     3541 gcccagtgcc acggaattga gcttcaggta cgcatcctcc ggcgaggcgc tcgcgccatt
     3601 ggcggaatcc gtcaccggga tgccgaccag atcgccaacg aacctcacgc tctgcggaaa
     3661 gctgagccct tcaatgcggg atatgaacga aaagacgttc cccccggcat gacagccgaa
     3721 acagtaaaaa atccgcttct cgaccgaaac ggtgaacgat ggcgttttct ccttatgaaa
     3781 ggggcatagg ccgataaaat ccttgccctt cttcgcaagc gacggaacat agcggcgcac
     3841 caccgattca atatcggccc gatcgcgaat gcggttgatg atttcgcgcg gaatctgcac
     3901 tcgggatgat cgtgcgccga ctatcggccc tcgagcttct tgccaaggag ggatttcacg
     3961 atagcgccgt ccgcacgccc cttgagctct ttcatgatca cgctcatgat cttgccaaag
     4021 tccttggcgg tcaccgcacc catctcctcg attttccggt cgatggcggc cgcgatcgcc
     4081 tcttctccca gctgttccgg gagatagcgg gcgaggatct cgagctcggc ggcttccttg
     4141 gcggctagct cgccccgtcc ggccttcgtg aactcctcga tggcctcctt gcgcttcttg
     4201 gaggcccggg cgaccacctc caggacggcc tcgtcggtca attcggccgt tcccttcgtt
     4261 ttctcgtaca taatgtcgga tttgagcatg cgaagcgttc gcaccacatc ctcgttcttc
     4321 gccttgatcg cctcatgcag atcgccggaa attgtgtcga gcagactcac ggatattctc
     4381 ctcgcccgat cgcccgccgc cctcgggtcg acgcagtgcc gatgcacatg cgtaccgccg
     4441 aactgagcgt gagcggatca agccattatt tgataaacgt tttgtgttgt gcgggatagg
     4501 gacatacaca ccaactcaca tatagtatac gcgcaaatag gacaagcgtc aataatggcg
     4561 ccttaaataa tcattattaa caaaaatttt tttagtaaaa accgctgcat gtgttcaatt
     4621 catttttctt gttgagcgcc atctccgctg ttgccgaata ttaactattc aatcgcaata
     4681 atgttgttga cgcggaagat ggccgcggat gccgtatcga gggctatccg gttagtccat
     4741 agagcatata ctacgatact gttaaaaaat caaatttact ggggataccg aatgaaaaaa
     4801 gcgggaatcg cactttccgt actgctgctg gcactctgct tcgtcctctt cctgtcgttc
     4861 gataaaatcg gttcgatctg gagccaaaac gtcaaaaaac aatcatcggc gcgcgctcgc
     4921 gaagctgcaa aggtcgcacc ggccgttccc gggttcccca gcttctacaa gggaatctat
     4981 ttaaccagca actcgggcaa ggacttcaaa aaactgcaaa aatacgtttc catggcccag
     5041 gaagggcgcc tgaacaccct ggtaatcgac gtgcaggact cccgaataaa ggaaagcgcc
     5101 atccccaagc agaacatcga ttactgcatc gaaaagggtt ttcacacggt tgcccgcgtg
     5161 gtggtgtttc ccgacggcct caaaaagtat ccggtcgatc cgcagtatat cgcgaacaag
     5221 ctggccgtcg cggagaacgc ctgcaagctg ggattccggg agatccagtt cgattacatc
     5281 cggtttaacg accacggtat cttgaaaaag atcccgctca aggaaaaata cgcattcatc
     5341 gagaacttcc tacggaaggc gcgcgcgcac ctcaagccct acaatgtccg catcgccgcc
     5401 gacgtctttg gccgcatccc gctcaacaag cacgatccca tcgggcagcg catggagggg
     5461 ctcgatgagg tcgccgacgt catctgcccg atggcctatc cctcgcacta cacctggagc
     5521 aaaaagctca tgtccgaccc gtacaccacg gtgttcatca catcgaaaaa ggcgaaggag
     5581 cgggtcacca gggcccacat cgt
//
LOCUS       DAORHF010000246         5537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_15030_length_5537_cov_4.018242, whole genome shotgun sequence.
ACCESSION   DAORHF010000246 DAORHF010000000
VERSION     DAORHF010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15030_length_5537_cov_4.018242"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2258)
                     /locus_tag="PKJ16_15690"
     CDS             complement(<1..2258)
                     /locus_tag="PKJ16_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV48485.1"
                     /translation="MKAATVQRQARIYLDFLSPGDSTKTVVRIIDDKNITALRDNGVK
                     TVFMVTSEVIELDSYIPIVRRFEGRGLAVRSVYIETLDPGRFLTVKETFREICARCAD
                     GNALILFYGGGFSGALIAGFLMLLGRDADGAISAVNGLHEGLIVHENETRFLREFQAH
                     LDELRAAGEPVVPGNSAASHDIAIYHNNAPLPAAPAPAGAKRKSSAAAKAKSPRPAAP
                     KKPHPEKTESGAKTAPVATDVKFWFSLRFKLVSIISFIIVASLSSMIFVATYFFLGDN
                     KIRVQENNHKVSEIIALKVKSDFQTIIDKSRIIASFMGREGARRTGTGDAEAFINSDR
                     EIIATFIETKAAETGRDPRMKAVYNTTLINRHQINRDDLPSINAANAKIFAKAFDGDT
                     VVHNVSQGFNQPVIAIAAPYLKDRSETIVSILVTYFRLDAVLKAFEISGITRAFMVNE
                     RGDIIAHPDGAIVVSGGNYLNLPIVTMMIKSKVDNGQTRYRDENRVAQLGSYKKIGLG
                     GCGVIATVEEAKAFEEVYNIQRRNIYLMVIVLTVSFLIVFFFGRTITEPVIRLVGATK
                     RIKEGDYLVDISATTRDEIGALTTSFVEMGSGLAEREKLKSAFGKFVNKEIAEAVLRG
                     DIRLGGERKTVAVLFSDIRSFTSISEKLQPEEVVEFLNQYMSRMVDCVNRTNGTVDKY
                     IGDAIMAIWGAPVSKGNDAENAVNGALLMRAALIDFNKGRGGDKKPIIKIGCGINVGQ
                     VLAGQIGSEERM"
     gene            2387..3196
                     /locus_tag="PKJ16_15695"
     CDS             2387..3196
                     /locus_tag="PKJ16_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HNV48486.1"
                     /translation="MKNDLILYERKGHIALVTLNRPDRRNAFHEAMWTALDRVVDDIR
                     AALPRAIVLTGAGPAFCAGFDVNPDNTQVSGMIEAVQRHEREPAERLVARIRSAVDGF
                     VSLPVPIIAALNGLAYGGGAELAVRCDMRIADPDAVICFSETKLGLMPDWGGGAALAR
                     LIGPSRAADLVCTARRVDAGEAFALGLVNRISAPGETRYEALALAESIAQNGPRAVRA
                     ALSVVRRANDMPLPDALDYESEQAATLIASGECIHGISAFLTKQKAEFPDD"
     gene            complement(3491..5323)
                     /locus_tag="PKJ16_15700"
     CDS             complement(3491..5323)
                     /locus_tag="PKJ16_15700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48487.1"
                     /translation="MGSFFKILRFILPYKFIFIVAVLFSILFSLSNGVTIYAIVPLFD
                     TLSDKDRTTLFIVPESERALIAKDGKTLYEHAVATQAGAKLFVNDRLAGKSKLEMLTA
                     LAFAVVPLIFLRALFDFIARALFSYAGNRAVLNIRRRLFLHLLQLPYAYFHRSRSGEL
                     MSRITKDVMPLTTAVSGEVYNFLAGFLLIITNVTILAFVSWKLIIFILVVGPLIALPI
                     NFFSNKVRRYTRKIQEGFADISAHLNESIGGIKVIKSFAMEHFEREQFDEINRAIFLR
                     DLKKRIFQNLNPGIVQVLGSSIAVALFAFGGYQIINGELSSSEFVFFLILVLNLFEPT
                     QMISNATNEMKSAEAASERIFMILDFPTEEYQKGRAGTFERSIGLRGLSFTYADEQVL
                     HGIDLEIPKGRTVAIVGPSGSGKSTIINLIPGFYYPTAGALEFDGTDNRELTLDWLRE
                     KTSMVTQEVFLFNGTVLENITCGAPSTRERVEEAARIAHAHDFIMRLPNGYDTEVGER
                     GVLLSGGERQRITIARAIFDNPEILLFDEATSALDAESERAIQDSLEYLFKTRTSVVI
                     SHRLSTIQNADIIYVIERGRIIDRGSHAELLERCPTYRLLFTQG"
     gene            complement(5328..>5537)
                     /locus_tag="PKJ16_15705"
     CDS             complement(5328..>5537)
                     /locus_tag="PKJ16_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV48488.1"
                     /translation="ERLMNDRTSFVIAHRLSTVTGADRIIVLERGAIVETGTHDELLA
                     KGGIYKSLYEIQFADTPERAAREEA"
BASE COUNT         1088 a         1543 c         1732 g         1174 t
ORIGIN      
        1 tccatgcgct cctcggagcc gatctggccc gccaggacct gcccgacatt gataccgcat
       61 ccgatcttga tgatcggctt tttatctccg ccgcgcccct tgttgaaatc gatgagcgcc
      121 gcgcgcatca acagggcgcc gttcaccgca ttctcggcgt cgttgccctt cgagaccggg
      181 gcgccccata tcgccatgat cgcgtcgccg atgtatttgt caaccgtgcc attggtccgg
      241 ttcacgcaat cgaccatgcg gctcatgtac tggttcagaa actcgaccac ctcctccggt
      301 tgcagctttt ccgagatcga cgtgaaggag cggatgtcgg agaagaggac cgcgacggtc
      361 ttgcgctcgc cgccgagccg gatgtcgccg cgcagcaccg cctcggcgat ttccttgttc
      421 acgaacttac cgaaggccga cttgagcttc tcgcgctcgg cgagccccga tcccatctcc
      481 acgaacgagg tggtaagcgc gccgatctcg tcgcgcgtcg ttgcgctgat atccacgaga
      541 tagtcgccct ccttgatgcg cttggtggcg cccaccagac ggatcaccgg ttccgtgatc
      601 gttcggccga aaaagaacac gatgaggaat gagacggtaa ggacgatgac catgagatag
      661 atgttgcgcc gctggatatt gtagacctcc tcgaaggcct tggcctcctc gacggtcgcg
      721 atcaccccgc agccgccgag cccgatcttt ttgtacgagc cgagttgggc gacgcggttc
      781 tcgtcccgat agcgggtctg gccattgtcc accttgcttt tgatcatcat ggtcacgatg
      841 ggcagattga ggtaattgcc gccggagacg acgatggcgc cgtccgggtg cgcgatgatg
      901 tcgccccgct cgttgaccat gaacgcgcgc gtgatccccg agatctcgaa ggccttgagc
      961 acggcatcga gacggaaata ggtgacgagg atcgacacga tcgtttccga gcggtccttg
     1021 aggtacggag cggcgatggc gatgaccggc tggttaaagc cctgggacac gttgtgtacc
     1081 acggtgtcgc cgtcgaacgc cttcgcgaaa atcttcgcat tggcggcgtt gatggacggg
     1141 agatcgtcgc ggttgatctg gtgccggttg ataagcgtcg tgttgtagac ggccttcatg
     1201 cgcggatctc ggccggtttc cgcggccttc gtttcaatga aggttgcgat gatctcccgg
     1261 tcgctgttga tgaacgcctc ggcgtcgccc gtacccgtgc gacgggcgcc ctcgcgcccc
     1321 atgaacgatg cgatgatcct cgacttgtcg atgatcgtct gaaagtccga tttcaccttg
     1381 agcgcgatga tctcggatac cttgtgattg ttttcctgta cgcggatctt gttgtcgcca
     1441 aggaaaaagt aggtggccac gaagatcatg ctcgaaagcg acgcgacgat gatgaacgag
     1501 atgatggaga cgagcttgaa gcgaagcgag aaccagaact tgacgtcggt cgccacgggt
     1561 gcggttttcg cgccgctctc ggttttttcc gggtgcggct ttttgggggc tgcggggcgt
     1621 ggcgatttcg cctttgccgc agcgctcgat tttcttttcg caccggcggg ggcgggtgcg
     1681 gcggggaggg gcgcgttgtt gtgatagatc gcgatgtcat gcgaggccgc gctgtttccg
     1741 ggtacgacag gttcgccggc agcgcgcaat tcgtcgagat gcgcctgaaa ctcgcgcaaa
     1801 aaacgagttt cgttctcgtg cacaataaga ccttcatgca gcccgttcac tgccgatatc
     1861 gcgccgtctg cgtcacgtcc caacagcatc agaaaaccgg caataagcgc accggaaaat
     1921 ccgcctccgt agaagaggat gagggcattg ccgtcggcgc agcgtgcgca tatttcgcgg
     1981 aaggtctctt ttacggtcag gaagcgaccc ggatcaagcg tctcgatgta cacgctgcgc
     2041 accgcgagtc cgcgaccctc gaagcgtcga acgatcggga tgtaggaatc cagctcgata
     2101 acctccgagg tcaccatgaa gacggttttg actccgttgt ccctgagcgc ggtgatgttc
     2161 ttgtcgtcga tgattcgcac gacggttttc gtggaatcac ccggcgaaag aaaatcaaga
     2221 tatatgcgag cttgtcgctg aacggtggcg gctttcatgg caccctcgaa atatatttct
     2281 tcttattata tattatagtg tgattgtccc ggtaaattta gtagaaatag ggcgaataat
     2341 ccattttttt catggacaaa aaatgttcga tgttgattgt gaaagcatga aaaatgatct
     2401 gatcctttac gaacgaaaag gccatatcgc gctggtcacc ttgaatcgcc cagaccgcag
     2461 gaacgcattt cacgaggcga tgtggacggc cctcgatcgg gttgtcgacg acatacgagc
     2521 cgcgcttccc cgtgcaatcg ttctcaccgg ggccgggccg gccttttgcg cgggattcga
     2581 cgtcaatcct gacaatactc aggtttcggg gatgatcgag gccgtccagc gtcatgagcg
     2641 ggaacctgcc gagcggctcg tcgcccgcat acggtcggcg gtcgatgggt tcgtctcgct
     2701 gccggtgccc atcattgcgg cgctcaacgg cctcgcgtac ggcggcgggg cggagctcgc
     2761 ggttcgctgc gatatgcgca tcgccgatcc ggatgccgtc atatgtttct ccgaaacgaa
     2821 gctggggctc atgcccgatt ggggcggcgg ggctgctctt gccaggctca tcggtccctc
     2881 ccgcgccgcg gacctcgtat gcaccgcacg cagggtcgat gccggtgagg cgtttgcgct
     2941 tggactggtg aaccggataa gcgctccggg agagacgcgc tatgaggcgc tggcgttggc
     3001 ggaatcgatc gcgcaaaacg gcccgagggc ggtccgcgca gcactgtccg ttgttcgccg
     3061 ggcgaatgac atgccgcttc ccgacgcgct tgactacgaa tccgaacagg cggcaaccct
     3121 gatcgcgtcg ggcgagtgca ttcatggaat atcggcgttt ctgacaaagc aaaaggcgga
     3181 gttcccggat gattgatgat gggctcggaa aacgcgagag ccatcgcctt tcggccgatg
     3241 gctctcttaa gactgtgaca tgtaacacta acccttaaag aatctcattc ggtgggttga
     3301 cacgtaacgt cttcgatttg gaagaggctg tcggagcatc tatcgctcac actgcccgta
     3361 taaactcacc gaatcacaat aagaatatat agcatgaaat gcaaatcgtc aacagcgttg
     3421 ttgaaaaaat tctatcgaat tcaaaaaaac cggcgctttc gggtatcgtt tctcggatga
     3481 gaaacgatga ctacccttgc gtaaagagca gccggtaggt cgggcagcgt tcgagaagct
     3541 cggcgtgaga gccgcgatcg atgatccgcc cgcgctcgat tacgtaaatg atgtcggcgt
     3601 tctggatggt cgacaggcgg tgcgagatga ccaccgacgt gcgcgttttg aacaggtact
     3661 ccagcgagtc ctgtatggcg cgctccgatt ccgcgtcgag cgcggatgtc gcctcgtcga
     3721 agagcaggat ttcgggattg tcgaagatgg cgcgggcgat ggtgatgcgc tggcgctcgc
     3781 cgcccgagag cagcaccccg cgctctccca cctcggtgtc gtagccgttg ggaaggcgca
     3841 tgatgaagtc gtgcgcatgt gcgatgcgcg ccgcctcctc gacccgctcg cgcgtgctgg
     3901 gcgcgccgca ggtgatgttc tccagtaccg tcccgttgaa cagaaacacc tcctgagtga
     3961 ccatagaggt tttctcgcgg agccagtcca gcgtgagctc gcggttgtcc gtcccgtcga
     4021 attcgagcgc tcccgccgtc gggtagtaga atccgggaat caggttgatg atggtcgatt
     4081 tccccgaacc ggagggaccg acgatggcaa ccgtacgccc cttgggaatc tcgagatcga
     4141 tgccgtgaag cacctgctcg tcggcatagg tgaaggaaag tccccggagg ccgatgctac
     4201 gctcgaatgt gccggcgcgc cctttctggt actcctcggt cggaaaatcg aggatcatga
     4261 aaatgcgttc cgacgccgcc tcggcggatt tcatctcgtt ggtcgcgttt gagatcatct
     4321 gcgtgggctc gaagaggttg agcacgagga tgaggaagaa aacgaactcg ctggacgaca
     4381 gctcgccgtt gatgatctga tacccgccga aggcgaacag cgccaccgcg attgacgagc
     4441 caagcacctg cacgatgccg ggattgaggt tctggaaaat cctttttttc aggtcgcgga
     4501 ggaagatcgc gcgattgatc tcatcgaact gttcccgttc gaagtgttcc attgcgaacg
     4561 atttgatcac cttgatgccg ccgatggact cgttgaggtg cgcgctgatg tcggcgaagc
     4621 cctcctggat cttgcgggtg tagcggcgca ccttgttgct gaagaaattg atcggcaggg
     4681 cgataagcgg tcccactacc aggatgaata tgatgagctt ccagctcacg aaggcgagga
     4741 tggtcacgtt ggtgatgata agcaggaacc cggcgaggaa gttgtacacc tcgccgctta
     4801 cggcggtcgt gagcggcatc acgtccttgg tgatgcgcga catgagttcg ccgctccggc
     4861 tccggtggaa gtatgcgtat gggagctgca gcagatgtag gaagaggcgg cgccgaatgt
     4921 tcagcactgc gcgattgccg gcatacgaga agagcgcacg ggcgatgaaa tcgaacagcg
     4981 cgcggaggaa gatgagcggc accaccgcga aggcgagcgc cgtgagcatc tcgagtttgc
     5041 tcttgcccgc cagccggtcg ttcacgaaca gcttcgcgcc cgcctgcgtc gcgacggcgt
     5101 gctcgtagag cgtcttcccg tccttcgcga tcagcgcgcg ctcggattcg ggaacgatga
     5161 agagggtggt gcggtcctta tccgagagcg tgtcgaacag cgggacgatc gcatagatgg
     5221 tgacgccgtt ggagagcgag aagagaatcg agaacagcac cgcgacgatg aagatgaact
     5281 tgtacgggag gatgaacctc agtattttga agaagctgcc cacggcgtta tgcctcctcc
     5341 cgggcggcgc gttccggcgt gtccgcgaat tggatctcgt agaggctctt gtagattccg
     5401 cccttcgcga ggagctcgtc atgggttccg gtctccacga tcgcgccgcg ttccagcacg
     5461 atgatgcggt cggcgccggt cacggtggag aggcgatggg cgatgacgaa cgaggtccga
     5521 tcgttcatca ggcgttc
//
LOCUS       DAORHF010000247         5484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_15262_length_5484_cov_5.686130, whole genome shotgun sequence.
ACCESSION   DAORHF010000247 DAORHF010000000
VERSION     DAORHF010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15262_length_5484_cov_5.686130"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..148
                     /locus_tag="PKJ16_15710"
     CDS             11..148
                     /locus_tag="PKJ16_15710"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HNV48489.1"
                     /translation="MFRIPLMEMCRDERELRSEVRITVIHELGHYFGFDEPDLERLGL
                     L"
     gene            complement(310..1674)
                     /locus_tag="PKJ16_15715"
     CDS             complement(310..1674)
                     /locus_tag="PKJ16_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HNV48490.1"
                     /translation="MKQICSYLCILFISIALSLGCAQKGGDDGGGGGGGSAGNATSME
                     SNDQQAGDTEFQFDTTTKVDLATTITDADTNAPIAQVSITVTDPQNRTINSGTTNADG
                     IADFTLVIENSIESVTVIVRHEQYEEFSFVVNDVKKLAEIARDIKLKKKVVVVPIVDS
                     DGDGVADADDEFPNEKKYAKSVKGAFTLVFEDLYPVPGDADFNDAVVKLTIEERINAQ
                     NKIAYIKITAQALASGAGYNNQFGINVLGSEYLLIANFKGDLNDGDGTRIDDPFQPSA
                     FIRVQEIVLDTPIDRSEIAPMPYDPFLIPNGVNWPRGNVDYKGEVHLPFVDSAYKNSG
                     GMVLDSTGFPWALLVPGNWHWPLSGRSIYDGYPDFRAWYESRGAVSANWYTNVVGQYV
                     YPYTLEGQLALTAASKSALVAFVTAKGFAYRYPIGIALFLVVGLLVSIFVYHHIRIGG
                     LKKQ"
     gene            complement(1850..3568)
                     /locus_tag="PKJ16_15720"
     CDS             complement(1850..3568)
                     /locus_tag="PKJ16_15720"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HNV48491.1"
                     /translation="MTAIMIRYIVLLAIATATTAALMVALAPAGSGLVFSGFALIANL
                     ALFVLVAAVDHYLLPRLRMLRRRAAGADAVPNVLGGERRAPRAIVVGAGEAGRTIVVE
                     YRKRGRDREVVGFVDDDPAKFGATVEGRRVLGSVDRLPELIVSHNVDEVIVAIPSAAG
                     ARIYRIITAVKAAHPGVMIRILPSVMRAFDTPLVPELREIDMADLIDRDEIVVDTGRI
                     RERFGGATVLVTGAGGSIGSELCRQMLRFSITRLVAIGHGENSIYELAKNLRDHLAAL
                     PDPPAVEYRIVDIKDYALLERVFAEHRPDVVFHAAAHKHVPLMEFNEAEAVQNNVGGS
                     YALLRLARKYRAGRFVMVSTDKAVNPTSVMGATKRITELLTLSFHRRYGLTTAIVRFG
                     NVIGSRGSIIPLFREQIARGGPVTVTHPDVERYFMSIPEASILVINAAAYAAGGEIFV
                     LDMGRQHRILEVAQRLIRLCGREPGRDIAITFTGLRPGEKLSEELHYAREDLSRTAND
                     KIFLLNQRRQGADLPALDRFVTAGIKRLPSLTSAEIRREIKRIVPECRFTPEQSRKRA
                     SDRLVT"
     gene            complement(3575..4723)
                     /locus_tag="PKJ16_15725"
     CDS             complement(3575..4723)
                     /locus_tag="PKJ16_15725"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012673735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HNV48492.1"
                     /translation="MNIPFHRARITPDEIDAVNEVLRSGWITMGRATLAFEERFAAAV
                     GAPHAIAVSSCTAALHLALVVLGVGPGDEVIVPADTFVATAEAVEYCGARPVLADIDR
                     DTHCVGPAALERAITPRTRAVIPVHFAGQPCDMAAIMDIAQRHGARVVDDAAHAFPAS
                     IGGAAIGSCADLTCFSFYATKTLTTGEGGMITTRNPEWADALATLRLHGMSRDAWRRY
                     SGETSWRYDVTRLGYKYNTTDIAAAIGLGQLARADALRDERERIAQRYSRAFAADAAL
                     IPYAIRPGGVCAWHLYPLKLNLDSLTVDRDRFMALMRERGVATSVHFIPLYHFTHFKG
                     RGWSPDGFPESEWVFAREISLPLYPGMTDAEVDYVIDAVLGIARANRR"
     gene            complement(4725..>5484)
                     /locus_tag="PKJ16_15730"
     CDS             complement(4725..>5484)
                     /locus_tag="PKJ16_15730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HNV48493.1"
                     /translation="VRARYKLILQIVAGVLVWAAGLSFNKFNFLNLFEINFGYFSVVI
                     TVFWVVAFVNAINLLDGMDGLASGIVFIANIFVFYIAFTTGNALVASMSMVMAGSILG
                     FYIFNFPPAKIFMGDGGAYFLGFIYATMPLMGVKKSSVATIFLIPLILLLVPMLDIVQ
                     VVLKRVKMGYNIFIADKNHLHHRLLGIGLSIRGVLVVVYGYTVMLGLTSVLMVRIDPH
                     HSLLLFVIIGLLMFLSLYLLNSAERIIERRDGSD"
BASE COUNT         1061 a         1723 c         1747 g          953 t
ORIGIN      
        1 ccgcatctac atcttccgca ttcccctcat ggagatgtgc cgcgacgagc gcgaacttcg
       61 ctcggaggtc cgcatcaccg tcatccacga gctcgggcac tacttcggct tcgacgagcc
      121 cgacctggag aggctgggac tgctgtaaga acctatccca gcagggggtc gaatgagacg
      181 gcagggctcc gcacgaacgc tcccggctca tcaacggaaa aagcccccga aggggctttt
      241 tccttttaag gctcgatgac aatcgcgtgt gcacaatcga atgcgcgcgt gcgcacacgc
      301 ggaatgcgac tattgctttt tcaagccgcc gatgcgaatg tggtgataga cgaaaatcga
      361 caccagcaac cctacgacaa ggaacaatgc gatgccgatg ggataccggt aggcgaaccc
      421 cttggcggtt acaaaagcaa ccagggccga tttcgacgcc gccgtgagcg ccagctggcc
      481 ctccagcgta tatggataca catactgtcc gaccacattg gtgtaccaat ttgccgaaac
      541 ggcgccgcgg gactcatacc aggcgcggaa gtcgggatat ccgtcataaa tgctgcgacc
      601 ggagagcggc cagtgccagt tgccggggac cagaagcgcc cacgggaagc cggtcgaatc
      661 gagcaccatg ccgccgctgt tcttgtacgc ggaatcgacg aaaggaagat gcacctcgcc
      721 cttatagtct acatttcccc gcggccaatt gacgccgttt ggaatcagga acgggtcgta
      781 gggcatgggc gcgatctccg agcggtcgat gggcgtatcg agcacgatct cctgcacgcg
      841 aataaacgct gacggctgga agggatcgtc aatccgcgtc ccgtcgccat cgttcaggtc
      901 gcccttgaag ttcgcgatca ggagatattc cgaaccgagc acgttgatcc cgaactggtt
      961 gttgtacccc gccccgctgg cgagcgcctg cgcggtgatc ttgatatagg cgatcttgtt
     1021 ctgcgcgttg atgcgctctt cgatcgtgag cttcacgacc gcgtcgttga agtccgcgtc
     1081 gcccgggacc ggatagaggt cctcgaacac cagggtgaac gcgcccttga ccgacttcgc
     1141 gtattttttc tcgttcggga attcgtcgtc ggcatcggca acgccgtcgc cgtcggaatc
     1201 gacgatggga acgaccacga cctttttctt cagcttgatg tcgcgcgcga tctccgcgag
     1261 cttcttcacg tcgttcacga cgaagctgaa ctcctcatac tgctcgtgac ggacgatgac
     1321 cgtgacgctc tcgatggagt tctcgatcac cagcgtgaag tccgcgatgc cgtccgcatt
     1381 ggttgtcccg gaattgatgg tgcgattctg gggatcggta accgtgatcg aaacttgcgc
     1441 gatcggcgca ttggtgtccg cgtcggttat cgtcgtggcg aggtccacct tggtggtggt
     1501 gtcgaattgg aactccgtgt cgcccgcctg ctggtcgttg gattccatcg aagtggcgtt
     1561 tccggccgat ccgccgcctc cgccgccgcc atcatcacca cccttctggg cgcatcccaa
     1621 agaaagcgca attgaaataa aaagaataca taggtacgaa catatttgtt tcatattacc
     1681 ctccacggat tgttctatac tccatggtac caacaggtcc aataatagtc aattaaaaaa
     1741 gcccaaatgg gcttatattt tatcacgaat attctaatcg gaataatact tttacatatt
     1801 aaaatataag ttttgatcct gcgcgctatg tggtaatcgt gcaggtagtc tacgtcacca
     1861 ggcggtccga ggccctcttt cgcgactgtt ccggcgtaaa gcggcactcg ggcacgatcc
     1921 ttttgatctc ccggcgaatc tcggcggagg tgaggctcgg aagccgcttg atgccggcag
     1981 tcacaaaccg gtcgagcgcc gggaggtcgg cgccctgccg gcgctggttc aggaggaata
     2041 tcttgtcgtt cgcggtgcgt gacaggtcct cgcgggcgta gtgcagctcc tccgagagct
     2101 tctcgcccgg ccgcagtccc gtgaacgtta tggcgatgtc gcgcccgggc tcccgcccgc
     2161 agaggcggat caaacgctgc gcgacctcca ggatccggtg ctggcggccc atgtccagca
     2221 cgaagatctc gcccccggcc gcataggccg cggcgttgat cacgaggatc gaggcctcgg
     2281 ggatggacat gaagtatcgc tcaacgtccg ggtgcgtgac cgtcacgggg ccgccgcgcg
     2341 cgatctgctc gcgaaacagg gggatgatgg agccgcggct cccgatcaca ttgccgaagc
     2401 gcacgatggc ggtggtgagc ccgtagcgac ggtgaaacga gagcgtgagc agctccgtga
     2461 tgcgcttggt ggcgcccatc acgctcgtgg ggttcaccgc cttgtcggtg gacaccatca
     2521 cgaagcgccc ggcgcggtac ttccgcgcaa gccgcaggag cgcgtaactg ccgcccacgt
     2581 tgttctgcac ggcctcggcc tcgttgaact ccatcagggg aacgtgcttg tgcgccgcgg
     2641 cgtggaagac cacgtccgga cggtgctcgg cgaacacccg ctcgagcagg gcgtaatcct
     2701 tgatgtcgac gatgcgatac tcgaccgcgg gcgggtccgg cagcgcggcg agatgatcgc
     2761 gcaggttctt cgcgagctcg tagatcgagt tttccccgtg gccgattgcg acgaggcgcg
     2821 tgatcgagaa acgaagcatc tgccggcaca gctccgaacc gatgctcccg cccgcgcccg
     2881 tgaccagcac cgtggccccg ccgaagcgct cgcggatgcg ccccgtgtcc acgacgatct
     2941 cgtcccgatc gatgaggtcg gccatgtcga tctcgcggag ctccggcacg agcggcgtgt
     3001 cgaaggcgcg catcaccgac ggcaggatgc ggatcatcac gcccggatgc gccgccttga
     3061 ccgcggtgat gatccggtag atccgcgcgc ccgccgcgga gggaatcgcg acgatgacct
     3121 cgtcgacgtt gtgcgatacg ataagctcgg gaaggcggtc cacggacccc aggacccggc
     3181 ggccctccac ggtcgcgccg aacttcgcgg gatcgtcgtc gacgaacccg acgacctcac
     3241 gatcgcggcc gcgcttgcgg tactcgacaa cgatggtccg gcccgcctcg cccgcgccca
     3301 cgacgatcgc ccgcggcgcg cggcgctccc cgccgagcac gttggggacc gcatcggcac
     3361 cggcggccct tcgccgcagc atccgaaggc gcggcagcag atagtgatcg acggccgcga
     3421 cgagtacaaa aagcgcgaga ttggcaatca gcgcaaagcc cgaaaagacg aggccgctgc
     3481 ccgcgggcgc gagcgcgacc atgagcgcgg cggtcgtcgc cgtggcgatg gcgagcagga
     3541 cgatgtagcg gatcatgatc gccgtcacgc cctctcacct ccggttcgca cgcgcgatgc
     3601 cgaggaccgc gtcgatgacg taatcgacct cggcgtcggt catgccggga tagagcggga
     3661 ggctgatctc gcgcgcgaag acccattcgc tctccggaaa cccgtccgga gaccagcccc
     3721 gtcccttgaa atgggtaaag tggtagagcg gaataaaatg cacgctggtg gcgacccccc
     3781 gctcccgcat gagcgccatg aaccggtccc ggtcgacggt cagcgaatcg agattcagct
     3841 tgagcgggta caggtgccag gcgcacacgc cccccggccg gatggcgtag ggaatgagcg
     3901 ccgcatcggc cgcgaaggcg cgcgaatagc gctgcgcaat gcgttcgcgc tcgtcgcgca
     3961 gcgcgtcggc ccgcgcgagc tgacccagac cgatcgcggc ggcgatgtcg gtggtgttgt
     4021 atttatagcc caggcgcgtg acgtcgtagc gccaggatgt ttcgccggag tagcgccgcc
     4081 acgcgtcgcg cgacatccca tgcaggcgga gcgtcgcgag cgcatcggcc cactccggat
     4141 tgcgcgtggt gatcatgccc ccctcgccgg tggtgagggt cttggtcgcg tagaagctga
     4201 agcaggtgag gtcggcacag gagccgatcg cggcaccgcc gatgctcgcc ggaaaggcgt
     4261 gcgccgcatc gtccaccacg cgggcgccgt gccgttgcgc gatatccata atggccgcca
     4321 tgtcgcaggg ctgccccgcg aaatggaccg ggattacggc ccgcgtgcgc ggggtgatcg
     4381 cgcgctcgag cgccgccggg cccacgcaat gcgtgtcacg gtcgatgtcg gccaggaccg
     4441 ggcgcgcgcc gcagtattcc accgcctcgg cggtcgccac gaaggtgtcg gcgggcacga
     4501 tcacctcgtc gccgggcccg acgcccagca ccacgagcgc gaggtgcagc gcggccgtgc
     4561 acgagctcac cgcgatcgcg tgcggcgcgc cgaccgcggc ggcgaatcgc tcctcgaagg
     4621 ccagcgtcgc gcgccccatg gtgatccacc cgctgcgcag cacctcgttc acggcgtcga
     4681 tctcgtcggg cgtgatgcgg gcgcggtgaa acgggatgtt cacgtcagtc gctcccgtcg
     4741 cgccgctcga taatgcgctc ggccgagttg agcaggtaca acgagaggaa catcagaagg
     4801 ccgatgatga cgaagaggag aagcgaatgg tgcgggtcga tgcgcaccat gagcaccgag
     4861 gtgagcccca acatgacggt gtagccgtac accaccacga gcaccccgcg gatcgacaat
     4921 ccgatgccga ggaggcgatg gtgcaggtgg ttcttgtcgg cgatgaagat attgtagccc
     4981 atcttcacgc gcttgaggac tacctgcacg atgtccagca tgggcaccag caggaggatg
     5041 agcgggataa ggaagatggt cgcgaccgac gatttcttca cgcccatcag cggcatggtc
     5101 gcgtagatga agccgaggaa atacgccccg ccgtccccca tgaagatctt cgcgggcggg
     5161 aagttgaaaa tgtaaaaccc gaggatcgag cccgccatca ccatcgacat ggaggccacg
     5221 agggcgtttc ccgtcgtgaa ggcgatatag aagacgaaga tgttcgcgat aaagacgatg
     5281 cccgaggcca gcccgtccat cccgtcgagc aagttgatcg cgttcacgaa cgcgaccacc
     5341 cagaagaccg tgatcaccac cgagaagtag ccgaagttga tctcgaagag gttcaggaaa
     5401 ttgaacttgt tgaagctcag tcccgccgcc cacacgagga cgcccgcgac gatctgcagg
     5461 atgagcttgt accgcgcgcg caca
//
LOCUS       DAORHF010000248         5347 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_15861_length_5347_cov_5.271920, whole genome shotgun sequence.
ACCESSION   DAORHF010000248 DAORHF010000000
VERSION     DAORHF010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5347
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15861_length_5347_cov_5.271920"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="PKJ16_15735"
     CDS             <1..768
                     /locus_tag="PKJ16_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48494.1"
                     /translation="TIATGESVEARLPAVPAGAAVRSIAGAGDIVWCVAATASEPSGT
                     LYRLSLADKRVEALGAAHDVCVVSGEVCVLAPAGASAVLRFRGRELPVAIPSPARIRE
                     VIDERLVVAGNGAEAEVFDLAAWRSVHLYSSTKRYAPPGEYNLVVRAAVRGGRPDRTG
                     MEFYKIFINGIEAGRTDTALPAAVKSFKTSVRTNEELVVVAERWVLDAGKKKYLRANN
                     VQQPAPQRITVPEKRITVLEISSDGARFEYLVGAVEE"
     gene            complement(933..1796)
                     /locus_tag="PKJ16_15740"
     CDS             complement(933..1796)
                     /locus_tag="PKJ16_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48495.1"
                     /translation="MKPGTAITSVVTAIIIAVFSVVCLAPIDAHAQQKKDDKDKTAEV
                     KDDKMGWKTRDYRPYIKAFDDLQKLSMEFAEKNLQLAINEYDKGMDALDDMENEVAKI
                     ESKEGKKKNLNERWHWQEIDRKNQTKRYIGRIKYSAKVKSVTNFTRAINLIDTIENSN
                     KKFITENKNYKIFKIKLFQVYVSTQYDLQNFKPCIPVLERYLSIDENTKTDIWAYRYL
                     ASCYGFMENVLAKYKKATEDEIIDYKQKKNTSLLKAAELKYTVESPQYKHLKDIVERD
                     EMKSESLNDFR"
     gene            2001..2819
                     /locus_tag="PKJ16_15745"
     CDS             2001..2819
                     /locus_tag="PKJ16_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48496.1"
                     /translation="MTRKSATADTAAKQFCSYCDHRGIIINPLYREMGENALLPCPKC
                     IQNTCACGGVDPYYFNDNGVIRECHCRSIRLKIDRIAVRYAQSGIDRKYRWRFINDFQ
                     PVSKAAEAAKAAAYDIIMKFPDVRKGLFLWGNPGTGKTLLSAIILTELIARSAIEGRF
                     IKVSRTFFERLRATFREGSESYGEAERIQQELAEADVLVIDDFGVQRDTPWEQETLYN
                     LVDARYEAERFTIFTSNNNPFSALKEFSDGRVLSRIKEMCRIIELAGADFRDKL"
     gene            2821..3300
                     /gene="folK"
                     /locus_tag="PKJ16_15750"
     CDS             2821..3300
                     /gene="folK"
                     /locus_tag="PKJ16_15750"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HNV48497.1"
                     /translation="MALAYLGLGSNLGDREANLRAARVMIISGCAGGLVRESVVEETE
                     PVDYLDQPRFLNQTVLLMTDRAPIELLDEAKRIEAALGRDYAIRRGPRTIDIDILLYD
                     DRVMDTERLTIPHPEITRRPFVMKQLIEIAPDLVDPRTGKLYREVYAHGGNRDHQRL"
     gene            3269..4072
                     /gene="panB"
                     /locus_tag="PKJ16_15755"
     CDS             3269..4072
                     /gene="panB"
                     /locus_tag="PKJ16_15755"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HNV48498.1"
                     /translation="MAAIATINDFKKAKENKEPIAMITCYEFAFARIIEQTGIDVILV
                     GDSLGMVVAGHTSTIPVTLDQMIYHAEIVRRGAPNAFIVCDLPFMSYHVSVEDALRNS
                     GRVMKVTGSNAVKLEGGRDFRATVEALVRASIPVMGHLGLTPQSVHKLGGYTVQGREE
                     DKRRIMIEDAKILEEAGAFSLVLEMVPEELAHEISRMLSIPTIGIGAGRYCDGQVLVI
                     NDLLGMDERFTPKFLKKYANLAEVAKSAIQGYCDEVKSGAFPAEGNVFK"
     gene            complement(4567..>5347)
                     /locus_tag="PKJ16_15760"
     CDS             complement(4567..>5347)
                     /locus_tag="PKJ16_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV48499.1"
                     /translation="IEKALDATRGGTRYNTSGSANIGLADVVDSLMAIKTLVYDERRI
                     SFAGLIEALATDFASAPELPALIRSRVPLFGSGNPHALEMARRVMRLVADAWHGHANF
                     RGGHYTAGFWSMSQHVAYGGLSGTLPSGRLRGTSFTPGLTPHPGASKSFLDNIRDVAG
                     LDASCMDNNIAFNVKLVPRPGEPVRDIVEAMYAYAKSYFDLGGMQMQFNVVSSETLRD
                     AMAHPENYRNLLVRISGYNAYFVTLNREMQIELIERAEYGI"
BASE COUNT          942 a         1616 c         1730 g         1059 t
ORIGIN      
        1 acgatcgcca cgggcgaaag cgtcgaggcg cgccttcccg ccgtgcccgc aggcgcggcg
       61 gtccggtcga tcgccggggc cggcgatatc gtgtggtgcg tcgccgcgac cgcctccgag
      121 ccgtcgggaa cgctctaccg gctttccctc gcggacaagc gagtcgaggc gctcggggcg
      181 gcgcatgacg tgtgcgtcgt gagcggcgag gtgtgcgtgc tcgcgccggc gggcgcgtcg
      241 gccgtgctgc gcttccgggg gcgcgagctt cccgtcgcga tccccagccc ggcgcgcatc
      301 cgcgaggtga tcgacgagcg gctcgtggtt gcgggcaacg gcgccgaggc cgaggtcttc
      361 gatcttgccg cctggcggag cgtgcatctc tattcgtcga cgaagcgata cgcgccgccc
      421 ggcgaataca atctcgtggt gcgcgccgcc gtgcgcgggg gacgccccga tcggacgggg
      481 atggagttct ataaaatctt catcaacggc atcgaggcgg gccggaccga cacggccctt
      541 ccggccgccg tgaaatcgtt caagacctcc gtgcggacga acgaggagct ggtggtcgtc
      601 gccgagcgct gggtgctcga cgcgggcaag aagaaatatc tgcgcgccaa caacgtgcag
      661 cagccggcgc cgcagcggat caccgtaccc gagaaaagga tcacggtcct cgagatctcg
      721 tcggacggcg cgcgattcga atacctggtg ggcgcggtcg aggagtaggc ggcgggcgcc
      781 gtcgggttct tcgccccttc gtcgccggag atatagcgcc ggtttttcca ctgaatgttt
      841 tatcggcagg tttttttcta caggcgcgcc tgccggcaca aaaacgcctc cccgtcgggg
      901 aggcgtttgg tttcgcgatg taatcgtacg atctagcgga agtcgttgag cgactcgctc
      961 ttcatctcgt cgcgctccac gatgtccttc aggtgcttgt actgcggcga ctcgaccgtg
     1021 tacttgagct cggcggcttt caggagcgag gtgttcttct tctgcttgta gtcgatgatc
     1081 tcgtcctcgg tggccttctt gtacttggcc agcacgttct ccatgaaacc gtagcagctg
     1141 gcgaggtagc ggtacgccca gatgtcggtc ttggtgttct cgtcgatgga gaggtagcgc
     1201 tccagcaccg ggatgcaggg cttgaagttc tgcaggtcgt actgcgtcga gacgtacacc
     1261 tggaagagct ttatcttgaa tatcttgtag ttcttgttct cggtgatgaa cttcttgttg
     1321 ctgttctcga tcgtgtcgat gaggttgatg gcgcgggtga agttggtcac cgacttgacc
     1381 ttcgcgctgt acttgatgcg gccgatgtac cgcttggtct ggttcttgcg gtcgatctcc
     1441 tgccagtgcc agcgctcgtt gaggttcttc ttctttcctt ctttactctc gatcttggcg
     1501 acctcgttct ccatgtcgtc cagcgcgtcc atgcccttgt cgtattcgtt gatggcaagc
     1561 tggaggtttt tctcggcgaa ttccatgctc agcttctgga ggtcgtcgaa ggccttgatg
     1621 taggggcgat agtcccgggt cttccagccc atcttgtcgt ccttgacctc ggcggtcttg
     1681 tccttgtcgt ccttcttctg ctgtgcgtgg gcgtcgatgg gcgccaggca cacgaccgag
     1741 aaaacggcga tgatgatggc ggtcacgacg ctggtgatgg cggttccggg tttcatgtac
     1801 gatacctcgt tggttaatat catgtgcaac aggattgctc cttgtatgta tcggcccgag
     1861 aggggccaaa attgattctt tttttatttt gttgacacgc gccgcaggcg ctcctctgat
     1921 accccttcac gggccctgcg ggggaaatat acccacgcat cgggaaaacg taaagaaaat
     1981 gttccaaatc cgggcattcg atgaccagga aatccgcgac agccgatacc gccgcgaaac
     2041 aattctgctc gtattgcgat catcgcggca tcatcatcaa ccccctgtac cgcgagatgg
     2101 gggagaacgc gctcttgccc tgcccgaagt gcatccagaa cacgtgcgcc tgcggcggcg
     2161 tggaccccta ttacttcaac gataatggcg ttattcgcga gtgccactgc cggtccatcc
     2221 gcctcaagat cgaccggatc gccgtgcgct atgcgcaatc cgggatcgac cggaaatacc
     2281 gctggcgctt catcaacgac ttccagccgg tgagcaaggc ggccgaggcg gccaaggccg
     2341 ccgcctacga catcatcatg aaatttcccg acgtgcgcaa ggggctgttc ctatggggca
     2401 atcccggcac cgggaaaacg ctgctctcgg cgatcatcct caccgagctc atcgcgcgct
     2461 ccgcgatcga ggggcgtttc atcaaggtgt cgcgcacctt cttcgagcgc ctgcgcgcca
     2521 ccttccgcga gggctccgag agctacggcg aggccgagcg catccagcag gagctcgccg
     2581 aggccgatgt cctggtgatc gacgatttcg gtgtgcagcg cgacaccccc tgggagcagg
     2641 agacgctgta taatctggtg gacgcgcgct acgaggcgga gcgcttcacc atcttcacct
     2701 cgaacaacaa cccgttctcg gcgctcaagg agttctccga cgggcgagtg ctctcgcgca
     2761 tcaaggagat gtgccgcatc atcgagctcg cgggggccga cttccgcgac aagctgtagg
     2821 atggcgctgg cgtatctggg gctggggtcc aatctgggcg accgggaggc gaatctccgt
     2881 gcggccaggg tgatgataat ctccggctgc gcgggcgggc tggtccggga gagcgtcgtc
     2941 gaggagacgg aaccggtgga ttacctcgac cagccgcgct tcctgaacca gacagtgctg
     3001 ctgatgaccg accgcgcacc gatcgaactt ctggacgagg cgaagcggat cgaggccgcg
     3061 ctggggcgcg actacgcaat ccggcgcggt ccgcgcacca tcgacattga cattctgctg
     3121 tacgacgacc gagtgatgga caccgaacgg ctgacgatcc cgcatccgga gatcacgcgc
     3181 cggccgttcg tgatgaagca gctcatcgag atcgcgcccg atctcgtcga tccccgcacg
     3241 gggaaactgt accgggaggt atacgcgcat ggcggcaatc gcgaccatca acgactttaa
     3301 gaaggcgaag gaaaacaaag agcccattgc gatgatcacc tgctacgagt tcgccttcgc
     3361 gcgcatcatc gagcagaccg gcatcgacgt gatcctcgtg ggcgactcgc tgggcatggt
     3421 ggtggcgggc cacacaagca ccatcccggt cacgctggac cagatgatct accacgccga
     3481 gatcgtacgc cgcggcgcgc cgaacgcgtt tatcgtgtgc gacctgccgt tcatgagcta
     3541 tcacgtgtcg gtggaggacg cgctgcgcaa ttccgggcgc gtgatgaagg tgaccggctc
     3601 gaacgccgtc aagctcgagg gcgggcgcga tttccgcgcg accgtggagg cgctggtgcg
     3661 cgcctccatc ccggtgatgg ggcacctggg cctcacgccg cagtcggtgc acaagctggg
     3721 cggctacacc gtgcagggcc gcgaggagga caagcggcgc atcatgatcg aggacgcgaa
     3781 gatcctcgag gaggccggcg ccttctcgct ggtgctggag atggtgcccg aggagctcgc
     3841 gcacgagatc tcgcggatgc tgtccattcc cacgatcggc atcggcgccg ggcggtactg
     3901 cgacgggcag gtgctggtga tcaacgacct cctgggcatg gacgagcgct tcaccccgaa
     3961 gttcctgaag aaatacgcga acctcgccga ggtcgcgaag agtgccatcc agggctattg
     4021 cgacgaggtg aagtccggcg cgtttcccgc cgaggggaac gtgttcaagt agggggcgag
     4081 accattgtac ggtcctcttc ttcgatgatg cggtatatgc cccgtctcgc aataagaacc
     4141 tgcacggtct ttgtgttgat ggtcttcctg tcctgcggat ctaacaggcc aaaaccggac
     4201 ggtcgcggat gggtcaagtg tccccgctgc cattcgcggg ggagcatcga atattttgac
     4261 aaccgagatc atccggatgc gatcgtttca tccccggatc cgcacagccc ggcatttacc
     4321 ggcgatacct cgaactgctg cctgtctggt accggctcgg gcacaaccga tatcaacaga
     4381 aaatacatcg agaagctcga tgccgacagc acgaaatacg gcgacagcgg acgcggtgtg
     4441 cgctacaagg cgtgtcccgt atgcaacggc gtcggctgga taaaggacag aaatcgattg
     4501 tataattatc ctggattgta ttgacggctc ggggtggttt ggccgccggt acgtccaggt
     4561 ggcccgctag atcccgtatt ccgcccgctc gatgagctcg atctgcatct cgcggttgag
     4621 cgtcacgaaa tacgcattgt agcccgatat gcggaccagc aggttccggt agttctccgg
     4681 gtgcgccatc gcgtcgcgca acgtctccga tgaaaccacg ttgaactgca tctgcatccc
     4741 gccgagatcg aaatagcttt tcgcataggc gtacatcgcc tccacgatgt cgcgcaccgg
     4801 ctcgcccggg cgcggcacga gcttcacgtt gaatgcgatg ttgttgtcca tgcacgaggc
     4861 gtcgagcccc gctacgtcgc ggatgttgtc caggaagctt ttcgacgccc cgggatgcgg
     4921 cgtgaggccc ggcgtgaacg aggtcccgcg caggcggccc gagggcagcg tgccggacag
     4981 gccgccgtag gcaacgtgct gggacatgga ccagaagccc gcggtgtagt ggccgcccct
     5041 gaagttcgcg tgcccgtgcc aggcgtcggc gaccagccgc atcacgcgcc gcgccatctc
     5101 gagcgcgtgc ggattcccgg agccgaacag gggtacgcgc gagcggatga gcgccggcag
     5161 ttcgggggcg ctcgcgaaat cggtggcgag cgcctcgatg agaccggcaa acgagatgcg
     5221 ccgctcgtcg tatacgaggg tcttgatcgc catcagggag tccaccacgt cggccagtcc
     5281 gatgttcgcg ctgcccgagg tgttgtagcg cgtgccgccg cgcgtcgcgt cgagcgcctt
     5341 ctcgatg
//
LOCUS       DAORHF010000249         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_15932_length_5331_cov_5.114670, whole genome shotgun sequence.
ACCESSION   DAORHF010000249 DAORHF010000000
VERSION     DAORHF010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15932_length_5331_cov_5.114670"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PKJ16_15765"
     CDS             complement(<1..417)
                     /locus_tag="PKJ16_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HNV48500.1"
                     /translation="MKKLILITCALGMTLYGMAIRPAPGSGSVRWHRFDAGLQIATAQ
                     KKPIVIDFFSVNCGWCKVMERETFSNAAVLGKLSGHYVPIRIDMDRNESFTFNGRTVS
                     AREFAMMLGVQGLPTIVFMDKNGKFITKIPGYIKTDV"
     gene            657..1343
                     /locus_tag="PKJ16_15770"
     CDS             657..1343
                     /locus_tag="PKJ16_15770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48501.1"
                     /translation="MLDIQIQEALEKNKKVKLIAYSVSPTIEERTKRVLRHFLERCGC
                     VELHGAVYTVVKELLINAVKANYKNIYFEDYNPKNRAAELIGYNTALQLFKLELNRDE
                     SDYFVRAARRKGICAEMVIGLERDTLVVSVTNPVPMTDIEMENVVRKLFDAGNCECMA
                     DYFLINEDDPNTEGAGLGLVLVTMILKSLGVDLSTFGVVSRDGKTEASFRVMLNEDTM
                     RQYEAATKAN"
     gene            1356..2159
                     /locus_tag="PKJ16_15775"
     CDS             1356..2159
                     /locus_tag="PKJ16_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HNV48502.1"
                     /translation="MERRIDIAFSPCPNDTFMFHALVHGAIDVRGWRFDAHIADVEEL
                     NRAALAARFPVSKLSAAAYLRVKDAYAILDTGAALGFGCGPLVVSREARSRIDGMTVA
                     VPGEHTTAHLLFRLWCDGETRVVTARYDEIMPGVADGRFDAGVIIHEGRFVYPAYGLV
                     KVVDLGEWWERETGGPIPLGCVAARRIDIDDAARCDLETLLRASIDHGMRNPAASRGF
                     IRAHAQEMDDGVIDRHIALYVNEFSRSLGDAGRRAIDVLESRARERGLL"
     gene            2156..2767
                     /locus_tag="PKJ16_15780"
     CDS             2156..2767
                     /locus_tag="PKJ16_15780"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48503.1"
                     /translation="MTGIVMATYLEAAPFVRGLNLRRSGKKPFRVYDGEHYRLILSGI
                     GKANAAMATAVLALDGDVDTVINAGAAGALRDGFALGDIRVIDRVVEGDRPRLRDGGP
                     RIVTPDPVEGHAGATLITHDRPILKPADRARAAAVADLADMEGAAVVQACRTFGLRCH
                     LVKIVTDTPEHSTSVHIIFNIRRTRRGLYEYCRTAFFAPGIIT"
     gene            2838..3074
                     /locus_tag="PKJ16_15785"
     CDS             2838..3074
                     /locus_tag="PKJ16_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48504.1"
                     /translation="MTFERENIGYFIAFMLIGGILGSALGTLIAKLAPAASIVKADLT
                     GAIGVNLEIVSFSIKLNIAAIVGIVAGIAIFRKL"
     gene            3385..4203
                     /gene="rpsB"
                     /locus_tag="PKJ16_15790"
     CDS             3385..4203
                     /gene="rpsB"
                     /locus_tag="PKJ16_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HNV48505.1"
                     /translation="MSVVTMKALLESGVHFGHQTRRWNPKMQQFIFTARNGIHIIDLQ
                     KTMQRLKIAYSAMKEVAERGGRVLFVGTKKQAQSAIEEYAKKCGMYYVADRWLGGLLT
                     NYQTVSRSIQRLKELERMSETGQWDAETKKEVLELQKELAKKNKVLSGIKDLGRLPDA
                     IFIIDPKREYIVVQEARKLRIPIFAVVDTNCNPDEIDFPIPGNDDAIRAIALFLDVMA
                     RAIVEGQSGGQSEDVIEGGIDADDTNDAADEDDLADDDATSGDSRKLGNGEVSG"
     gene            4252..4848
                     /gene="tsf"
                     /locus_tag="PKJ16_15795"
     CDS             4252..4848
                     /gene="tsf"
                     /locus_tag="PKJ16_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HNV48506.1"
                     /translation="MAEVTSELIKELREKTQAGMLDCKKALVETGGDLEKAAELLRKK
                     GLASANKRVDRVAKEGIIASYIHGNAKLGVLLELNCETDFVARNEDFQGLAKELCMQV
                     AAANPLYVDIEDVPAEELEKEKGIYREQMKDSGKPDNVVEKIIEGKLKKYYSEVCLLE
                     QPYIKDDKVVVRELVKDKIAVYGENITVGRFTRFKIGK"
     gene            4851..>5331
                     /locus_tag="PKJ16_15800"
     CDS             4851..>5331
                     /locus_tag="PKJ16_15800"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0044210 - 'de novo' CTP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine monophosphate kinase"
                     /protein_id="HNV48507.1"
                     /translation="MDSESGPHPRYRRILIKLSGEALAAEGGFGISPSTVKRIAGEVA
                     AVFSAGVQVAVVIGGGNIFRGSIGTALGMERTTGDSMGMLATVINSLAMQDALEKAGV
                     PTRVMTAIEMRAVAEPYIRRRAIRHLEKGRAVILSAGTGNPFFTTDTAASLRAIEIGA
                     "
BASE COUNT         1065 a         1605 c         1712 g          949 t
ORIGIN      
        1 cacgtcggtc ttgatgtagc cggggatctt ggtgatgaac ttgccgttct tgtccatgaa
       61 cacgatggtc ggcaggccct ggacgcccag catcatggcg aactcccgcg cgctcacggt
      121 ccgtccgttg aacgtaaaac tctcgttcct gtccatgtcg atacggatgg ggacatagtg
      181 gccgctgagc ttcccgagca ccgccgcgtt cgaaaaggtc tcgcgctcca tcaccttgca
      241 ccagccgcaa ttcaccgaaa agaagtcgat gacgatcggc ttcttctgcg cggtcgcaat
      301 ttgcaatccg gcgtcgaagc ggtgccatcg caccgagccg ctccccgggg ccgggcgtat
      361 cgccatgccg tacagggtca tgcccagtgc gcaggtaatc aggatcaact tcttcattat
      421 aagcacctcg ctggttttgc tcgcgactat ccaatgatac tatagcgcgc cggcccgctg
      481 tcgtacaggt tttccccggt tttgtcaaca cacaattgcc aaaacgcgcc atcgagacat
      541 ccggggcatg ccgatggcgc ccctgcgccg gggcgctttt tgtattgacg aaatcccatg
      601 agtgtgcatt gcatattcca cggaagcgga atgttgattg ggcggaatag taacatatgc
      661 tcgatataca gatacaagag gcgctcgaaa agaacaagaa ggtcaagctc atcgcctatt
      721 cggtgagccc cacgatcgag gagcgcacga agcgggtcct gcgccatttc ctcgagcggt
      781 gcggctgcgt cgagctgcac ggagcggtct acaccgtcgt caaggagctg ctcatcaatg
      841 ccgtgaaggc gaattacaag aacatctact tcgaggatta taatccgaag aaccgggcgg
      901 ccgagctcat cggctataat acggcgctgc agcttttcaa gctcgagctc aatcgcgacg
      961 agtccgacta tttcgtgcgt gcggcgcggc ggaagggcat ctgcgccgag atggtcatcg
     1021 gcctggagcg cgacacgctc gtggtgtccg tgacgaaccc cgtgcccatg accgacatcg
     1081 aaatggaaaa cgtggtgcgg aagctctttg atgcgggcaa ttgcgagtgc atggccgatt
     1141 acttcctcat caacgaggac gatcccaata ccgagggcgc gggattgggg ctggtccttg
     1201 tgaccatgat attaaaaagc ctcggtgtgg acctgtccac cttcggcgtg gtttcccgcg
     1261 acggtaaaac ggaggcctcg ttccgcgtga tgctcaacga agacaccatg cgtcagtacg
     1321 aagcggcgac gaaggcgaac tgatcgccgc tatccatgga acgccgcatc gacatcgcct
     1381 tttcgccatg ccccaatgat acatttatgt ttcatgccct cgtacatggg gcgatcgatg
     1441 tgcgcggatg gcgcttcgac gcgcatatcg ccgacgtgga ggagctcaat cgagcggcgc
     1501 tcgcggcgcg ctttccggta agcaagctct cggcggccgc gtacctgcgg gtgaaggacg
     1561 cctatgcgat tctcgatacc ggcgccgcgc ttggattcgg ctgcggcccg ctcgtggttt
     1621 cgcgcgaagc gcgctcgcgc atcgacggca tgaccgtcgc ggtgcccggg gagcacacca
     1681 ccgcgcatct gctgtttcgg ctgtggtgcg acggcgaaac gcgcgtcgtc acggcgcgct
     1741 acgacgagat catgcccggg gtcgcggacg ggcgtttcga cgccggcgtc atcatacacg
     1801 agggccggtt cgtgtatcct gcgtacggcc tggtgaaggt cgtcgatctg ggcgagtggt
     1861 gggagcgcga gacgggcggg cccatcccgc ttggctgcgt cgccgcgagg cggatcgaca
     1921 tcgatgatgc ggcgcggtgt gaccttgaga cgctgctgcg cgcatcgatc gaccatggaa
     1981 tgcggaaccc cgccgcatcg cggggattta tccgcgcgca cgcgcaggag atggacgatg
     2041 gcgtgatcga ccggcatatc gcgctgtacg tgaacgagtt ctcgcgctcg ctcggcgatg
     2101 cggggcggcg cgccatcgac gtgctggagt cgcgcgcgcg cgagcggggc ctgctgtgac
     2161 cggcattgtg atggcgacct atctcgaggc ggcccccttc gttcgggggc tgaacctgcg
     2221 ccgatcgggc aagaagccgt ttcgcgtgta cgacggcgaa cactatcgcc tcatcctctc
     2281 cggaatcggc aaggcgaatg cggcgatggc gacggccgtc ctcgcccttg acggcgacgt
     2341 ggacaccgtg atcaacgccg gtgcggcggg tgcgcttcgg gacggatttg cgttgggcga
     2401 catccgcgtg atcgaccggg tggtcgaggg cgaccggccg cgcctccggg acgggggccc
     2461 gcgcatcgtg acgccggacc ccgtcgaggg ccatgcgggc gcgaccctca tcacccacga
     2521 ccggccgatc ctgaaaccgg cggaccgcgc ccgcgccgcg gccgtcgccg acctcgcgga
     2581 catggagggc gcggcggtgg tgcaggcctg ccggaccttc ggcctccgct gccatctcgt
     2641 aaagatcgtg accgacacgc cggagcacag caccagcgtc cacatcatat tcaatatccg
     2701 ccgaacgcga cgcggcctct acgagtattg ccgcacggcc ttcttcgccc ccggaataat
     2761 cacttgacaa aaatagccgc gtgccgccat gtctcctgca gtttgcatgt ccgcgaatgc
     2821 ggaacatggg agcgcgcatg acattcgaga gagagaatat cggctatttc atcgccttca
     2881 tgctcattgg cggcattctc gggtcggcgc tgggaacgct catcgcaaag ctggcgcccg
     2941 cggcctccat cgtcaaggcg gacctcacgg gcgcgattgg cgtgaatctc gaaatcgtca
     3001 gctttagcat caagctgaac atcgcggcca tcgtcggcat tgtcgccggg atcgcgatct
     3061 tcagaaagct gtaatccgtt ggcggtcccc gggaccgcta cgggagcgaa accatggcgt
     3121 ttcgcggccg cacccgcggc tatcacgcgc accgggatat ggttcggtgc gcagaacgtg
     3181 aatcggcgtg tgatgcgtcg atgccacctt ccaaacgggt cgtcttagtt ttatttgaag
     3241 ttgattcgcg atgcgcaccg catcgatgcc gcgttcgggt gtccgaatgc gcggcggtcc
     3301 gcccggcgtt ttcacgggcg tggcggaggc gcatcgggaa tcgtgccgta cgaacaagaa
     3361 ctcaatcgtt tcagggggta taacttgtca gtagttacaa tgaaggctct attggaatcg
     3421 ggtgtccatt ttggccacca gaccaggcgt tggaatccca agatgcagca gttcatcttc
     3481 accgcgcgca acgggattca catcatcgat ctccagaaga cgatgcagcg gctgaagatc
     3541 gcgtacagcg cgatgaagga agtcgccgag cgcggcggcc gggtcctgtt cgtggggacc
     3601 aagaagcagg cgcaatcggc gatcgaggaa tatgcgaaaa aatgcggcat gtactacgtc
     3661 gccgaccgat ggctcggcgg actgctcacc aactatcaaa ccgtgagccg ttcgatccag
     3721 cgcctcaagg aactcgagcg catgagcgag accggccagt gggacgccga aaccaagaaa
     3781 gaggtgctcg agcttcaaaa ggagctggcc aagaagaaca aggtcctcag cggcatcaag
     3841 gacttgggac ggctccccga cgccatcttc atcatcgatc ccaaacgtga atatatcgtg
     3901 gtgcaggaag cgcgcaagct gcgcatcccc atcttcgcgg tggtggacac caactgcaat
     3961 cccgacgaga tcgattttcc gatccccggg aacgacgacg ccatccgggc gatcgcgctc
     4021 ttcctggacg ttatggcgcg cgccatcgtc gagggacaat cgggcgggca gtccgaggac
     4081 gtgatcgagg gcggaatcga cgccgatgac acgaacgatg ccgccgacga ggatgatctg
     4141 gccgacgacg acgcgacgag cggcgattcg cgaaagctcg gcaacggaga ggtctccggg
     4201 taatcggtcg cttcccgcgc acattttgat attgagacaa ggagtccgaa cgtggctgaa
     4261 gtaacaagcg aactaatcaa ggaactgagg gaaaaaacgc aggccggcat gctcgattgc
     4321 aaaaaggcgc tggtggagac cggcggcgac ctcgagaagg cggccgagct gttgcgcaag
     4381 aaggggctcg cctccgcgaa caagcgcgtc gaccgcgttg cgaaggaagg gatcatcgcc
     4441 tcctatattc acgggaacgc caagctgggc gttctcctcg agctcaactg cgagacggac
     4501 ttcgtcgcgc gcaacgagga cttccagggc ctcgccaagg agctgtgcat gcaagtggcc
     4561 gcggccaacc cgctgtatgt cgacatcgag gacgttcccg ccgaggagct cgagaaggag
     4621 aaggggatat accgcgagca gatgaaggac tccggcaagc cggacaacgt ggtggagaag
     4681 atcatcgagg gcaaactcaa gaagtactac agcgaggtgt gcctgctcga gcagccgtat
     4741 atcaaggacg acaaggtggt cgtccgcgaa ctggtgaagg acaagatcgc cgtgtacggc
     4801 gagaacatca ccgtgggccg cttcacccgc ttcaagatcg ggaagtgacc atggacagcg
     4861 agagcggccc gcacccgcga tatcgccgaa tcctcatcaa actctccgga gaggcgctcg
     4921 ctgctgaggg cgggttcggc atcagcccgt cgaccgtcaa gcggatcgcg ggggaggtcg
     4981 cggcggtgtt ctccgccggc gtccaggtcg cggtcgtgat cggcggggga aacatcttcc
     5041 gcggatccat cgggacggcg ctgggcatgg agcgcaccac gggcgattcg atgggcatgc
     5101 tcgcgacggt catcaattcg ctggccatgc aggacgcgct ggagaaggcc ggcgtcccca
     5161 cgcgggtgat gaccgccatc gagatgcgcg cggtggccga gccctacatc cggcgccggg
     5221 ccatccgcca tctcgaaaag ggccgcgccg tgatcctctc cgcgggcacc ggcaacccgt
     5281 tctttaccac cgataccgcc gcaagcctcc gggccatcga gatcggcgcc g
//
LOCUS       DAORHF010000250         5319 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_15990_length_5319_cov_4.721695, whole genome shotgun sequence.
ACCESSION   DAORHF010000250 DAORHF010000000
VERSION     DAORHF010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15990_length_5319_cov_4.721695"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /gene="mutL"
                     /locus_tag="PKJ16_15805"
     CDS             <1..1581
                     /gene="mutL"
                     /locus_tag="PKJ16_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HNV48508.1"
                     /translation="PAEDLPLAVREHATSKIVEINDIERVGSYGFRGEALSSISSISR
                     MTISTRTDGEALGARLVASDGSAEVSPFAGPVGTTIIVENLFYNVPARKKFLKAKGTE
                     LRYIRETLLGIALALPEISFTLTVDDKRQLVLPHAETPEQRIRDIYGAETAQGLILEH
                     LADLKAEITGYFSSPSFMRSSRSMQQLYVNRRPIEYPYLGFLLSRAYEGIAGHGSHPA
                     ALVFISIDPSLVDVNVHPAKREIKFFDQKYVDSLITSLARKALGGGAHAVRVARKETA
                     PEPFATRDAADHANTTTASSERAPSRTADEPRRLFGHTAQYDDAVSTQSVIRDARHLY
                     RASEAPDRRVLGVIFGTYLLAEDAESLIIIDFHAAHERMLFDRLMSAEYEPSVQGLAF
                     PVVVELSINEYAIIREQLARLREIGFDIDFMSDTTVSVQGVPDIARGIDPAAFVRDFA
                     ESIRTERDRHPDIRAAIMERIACHAAARANDHLTTEDMRALLDTVMSGRHETRCPHGR
                     PFLYRLGRSELEGLFKRS"
     gene            1578..2111
                     /locus_tag="PKJ16_15810"
     CDS             1578..2111
                     /locus_tag="PKJ16_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HNV48509.1"
                     /translation="MKKIILGVSSSIAAYKACDLTRLFVKAGYSVWCVLTQNATHLVT
                     PLSLEVLSGNPVYTDQWARDRREMGHIALKDDAALMAVVPATANAIGKFANGIADDLL
                     STTFLSVACPVVIAPAMNPNMWAHRAVRANIERLAGWGVRIVSPAEGLVACGDEGTGK
                     LADIDTIFQECVNAIGR"
     gene            2095..2808
                     /locus_tag="PKJ16_15815"
     CDS             2095..2808
                     /locus_tag="PKJ16_15815"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HNV48510.1"
                     /translation="MRLAGKKIIVTGGPTREWLDPVRFLSNASTGKMGVAIADEAARI
                     SGEVVFVHGPMREELYAGREYRMIGVETTVELRDAVVGQLAPGCVLIMAAAPADFTPV
                     ETSSKKIKKTAGADEIALRLKKTPDILKGVADIRHRDPGLRDIVVVGFAAETNDIETY
                     AKQKLVEKRLEMICLNDVGRVGAGFGTDTNVVTMFTKHGRRIELPLLSKEETARRILE
                     EVDQLMERSNQIGHSAGAY"
     gene            3059..3883
                     /gene="amrB"
                     /locus_tag="PKJ16_15820"
     CDS             3059..3883
                     /gene="amrB"
                     /locus_tag="PKJ16_15820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV48511.1"
                     /translation="MWTRHPIVAGSFYPSDPTRLRRDIEKYLGAAEAPPVKGSVVALI
                     SPHAGYVYSGPVAAYSFNQLSQVPFDVAIVLAPSHRARFEGASVIPEGVYKTPLGDVA
                     IDAQIGARLMEQPRFSFQRDAHSLEHSLEVQVPFLQVVREDFTLVPIIVGTTRLDVCA
                     EIANGIHAALTGERRNFAIVISTDLSHYFPYDEAVRTDTVFMDALRRFDERALSDVLS
                     QDKAQACGEGPVLTGMVLAKKLGATSVEIIKYMNSGDTAGGKDQVVGYLSAAFSAS"
     gene            3894..4475
                     /gene="amrA"
                     /locus_tag="PKJ16_15825"
     CDS             3894..4475
                     /gene="amrA"
                     /locus_tag="PKJ16_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNV48512.1"
                     /translation="MIELNRDQKIKLLALARATIGGYLGREAPSDESVAADYSDPVFQ
                     NRCGAFVTLHSRGNLRGCIGYIKGVKPIPDTIIDMAKASAFQDPRFRPLRAEELDAVD
                     IEISVLTPIEPLADVSDIVIGRDGLIISSGARSGLLLPQVASEHNWDVPTFLENTCYK
                     AGLPGDAWKKAGTKIERFSALVFGEKELGLPAR"
     gene            complement(4485..>5319)
                     /locus_tag="PKJ16_15830"
     CDS             complement(4485..>5319)
                     /locus_tag="PKJ16_15830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV48513.1"
                     /translation="ISFLYAIDRFSTPFTVDRCGRCGFIFMNPRFTDAALASFYDKGY
                     YTASAEYAYCDERAAERHARHVWNARLKTIRRHVSDGALLDVGCAFGGFLKAAQSRFR
                     PYGIEISPYAAGECRRWLGDALHQGTLADHPFPSGYFSVITMIEVIEHLADPVFAIEE
                     CHRLLRPGGLLVLQTANFDARQARRIRERYAYFLPGHVSYFTKRNLSDALRRAGFSRI
                     VAYQPVDFGLLPKLLKSRGGFRSIADYRAWFRIAWYHLQSTVRWNDFALTSSMVLYAI
                     K"
BASE COUNT          986 a         1664 c         1710 g          959 t
ORIGIN      
        1 ccggcggagg acctgccgct cgccgtgcgc gagcacgcaa ccagcaagat cgtcgagatc
       61 aacgatatcg agcgcgtcgg ttcgtacggg tttcgcggcg aggcgctgtc gagcatatcg
      121 tccatttcgc ggatgacgat atcgacgcgg accgacggcg aggcgctggg cgcgcggctg
      181 gtcgcgtcgg acggatcggc cgaggtgtcg cccttcgcgg gccccgtggg caccacgatc
      241 atcgtcgaga acctcttcta caacgtgccc gcccgcaaga agttcctcaa ggcgaagggg
      301 accgaacttc gctatatccg tgaaaccctg cttgggatcg cgctcgcgct tcctgagata
      361 tccttcacgc tcacggtgga cgataagcgg cagcttgtgc tcccgcacgc ggagacgccc
      421 gagcagcgca ttcgggacat ctacggcgcc gagacggctc agggcctcat cctcgagcac
      481 ctcgccgacc tcaaggccga gatcaccggc tatttctcct cgccgtcgtt catgcgctcg
      541 tcgcggtcga tgcagcagct ctacgtgaac cgccgtccca tcgagtatcc gtaccttggg
      601 tttcttcttt cgcgcgccta tgaggggatc gccggtcacg gatcacaccc ggcggcgctg
      661 gtgttcattt ctatcgatcc ttcgcttgtc gacgtgaacg tccatcccgc gaagcgcgag
      721 atcaagttct tcgaccaaaa atacgtcgat tcgctcatca ccagtctggc gcgaaaggct
      781 ctggggggcg gagcgcatgc cgttcgcgtt gcgcgtaagg agaccgcacc ggaaccattc
      841 gcgacgcggg atgcggcgga tcatgccaat accacgactg cttcgtcgga gcgcgctccg
      901 tcccgtacag cagacgaacc gcggcgtctt ttcgggcaca cggcgcagta tgatgacgcc
      961 gtatcgacgc aatcggtcat tcgcgacgcc cgtcacctct accgcgcgag cgaagccccc
     1021 gatcgccggg tgctcggcgt tatcttcggg acctatctgt tagccgaaga cgccgaatcg
     1081 ctcatcatca tcgatttcca cgcggcgcac gagcggatgc ttttcgaccg actgatgagc
     1141 gccgagtacg agccgtcggt acaggggctc gcgtttcccg tcgtggtgga gctgtcgata
     1201 aacgaatacg cgatcatccg cgagcaactc gcgcgcctca gggaaattgg gtttgacatc
     1261 gatttcatgt cggatacgac ggtatcggtg cagggggtcc cggacatcgc gcgcgggatc
     1321 gacccggcgg ccttcgtgcg ggatttcgcc gagtccatcc ggaccgagcg ggaccggcat
     1381 cccgatatcc gcgccgcgat catggagcgg atcgcctgcc acgcggccgc gcgcgcgaac
     1441 gaccacctca cgacggagga catgcgcgcg ctgctcgata cggtcatgag cgggcgccac
     1501 gaaacccgct gtccgcacgg gcggccgttc ctgtatcgcc tggggagatc cgagctggag
     1561 ggattgttca aacgatcatg aaaaagatca ttctgggagt ttcatcgtca atcgccgcct
     1621 acaaggcctg cgatctcacg cggctcttcg tgaaggcggg ctattcggtc tggtgcgtgc
     1681 tcacgcaaaa cgcgacgcac ctggtcacgc cgctttcgct cgaggtgctc tcgggaaacc
     1741 cggtctacac agatcagtgg gcgcgcgacc ggcgcgagat gggccacatc gcgctcaagg
     1801 acgacgccgc gctcatggcg gtcgttccgg cgacggcgaa cgcgatcggc aagttcgcca
     1861 acggcatcgc ggacgacctg ctgagcacga ccttcctgtc ggtggcctgt cccgtggtga
     1921 tcgcgcccgc gatgaacccg aacatgtggg cgcaccgcgc ggtgcgtgcg aacatcgagc
     1981 gcctcgccgg ctggggcgtg cggatcgtca gtcccgcgga gggactcgtc gcctgcggcg
     2041 acgaggggac cggcaagctc gccgacatcg acacgatttt tcaggagtgc gtcaatgcga
     2101 ttggccggta aaaaaatcat cgtgaccggc gggcccacgc gggaatggct cgatccggtg
     2161 cggttcctct ccaacgcctc caccgggaag atgggcgtgg ccatcgccga cgaggcggcc
     2221 cgcatatccg gcgaggtggt gttcgttcac gggcccatgc gggaggagct ctacgccggg
     2281 cgcgagtacc ggatgatcgg cgtcgagacc accgtcgagc tccgcgacgc ggtcgtgggg
     2341 cagcttgcgc ccgggtgcgt gctcatcatg gcggcggccc cggccgattt cacgccggtg
     2401 gagacatcat ccaaaaaaat caaaaagacc gccggcgccg atgagatcgc gctgcgttta
     2461 aaaaagacgc ccgacatcct caagggcgtg gccgacatcc gccaccgcga tcccggtttg
     2521 cgcgacatcg tcgtggtcgg gttcgcggcc gagaccaacg acatcgaaac ctacgcgaaa
     2581 cagaagctgg tcgaaaagcg gctcgagatg atctgcctca atgacgtggg acgcgtgggg
     2641 gcggggttcg ggaccgacac caacgtcgtg accatgttca cgaaacacgg ccgccgcatc
     2701 gagctgccgc tcctctccaa ggaggagacc gcccggcgca ttctcgaaga ggtcgaccag
     2761 ctcatggagc gcagcaacca gatcgggcat tcggcagggg cgtactaaga gccaatttca
     2821 gtaggagtcg agtgagacga cagtatgtca cgctcgcccc gccgcgaaca atacgtccat
     2881 gtattcgcgg ctccggcagc caatccgggc tgcctgctcg ctgtgacata ctgccgtctc
     2941 actcggtgct attggaaatg aaactactgt aataggttct aacatttact tgaccctgcg
     3001 atcccggcag tgatatattc gtgatgcggt ccggtgtggt cgcatagagg aggctgccat
     3061 gtggacaaga catccgatcg tcgccggttc gttttatccg tcggacccga cgcggttgcg
     3121 gcgtgatata gaaaagtacc tcggcgcggc cgaggcgcct cccgtcaagg gaagcgtcgt
     3181 cgcgctcata tcgccccatg cgggttatgt ctattcgggg ccggtcgccg catattcctt
     3241 caaccagctc tcgcaggtgc cgtttgacgt ggcgatcgtg ctcgcgccat cgcatcgcgc
     3301 gcgcttcgaa ggagcctccg tgatccccga gggcgtctac aagacccccc tgggcgacgt
     3361 ggcgatagac gcgcagatcg gcgcgcgcct catggagcag cctcggttca gcttccagcg
     3421 cgatgcgcat tcgctggagc attcgctcga ggtgcaggtg ccgttcttgc aggttgtccg
     3481 ggaggacttc acgctggtgc ccatcatcgt tggcacgacc cggctcgacg tgtgcgccga
     3541 gatcgcgaac gggatccatg cggcgctcac cggcgagcgc aggaacttcg cgatcgtcat
     3601 ctcgaccgat ctctcgcatt acttccccta cgacgaggcg gtgcggaccg acacggtgtt
     3661 catggatgcg ctgcggcgtt tcgacgagcg cgccctttcc gacgttctct ctcaggacaa
     3721 ggcccaggcc tgcggcgaag gcccggtgct tacgggcatg gttctcgcca agaagctcgg
     3781 ggccacgtcg gtggaaatca tcaaatacat gaactcgggc gacaccgctg gcgggaagga
     3841 ccaggtggtg ggctatcttt ccgccgcctt ttccgcctcg taggaggatg acgatgatcg
     3901 aactgaatcg cgaccagaag atcaagctgc ttgcgctcgc gcgcgcgacc atcggcgggt
     3961 acctcggccg cgaggcccca tcggacgaga gcgtcgcagc tgattattcc gacccggtgt
     4021 tccagaatcg ctgcggcgcc ttcgtgaccc tccacagccg cgggaacctg cgcggctgca
     4081 tcggctatat caagggcgta aagccaattc cggacaccat catcgacatg gcgaaggcct
     4141 cggcgttcca ggacccgcgc tttcgccccc tgcgcgccga ggagctcgat gcggtcgata
     4201 tcgagatatc ggtgctcacg ccgatcgaac cgctcgcgga cgtctcggat atcgtcatcg
     4261 gtcgggacgg gctcatcatt tccagcgggg cgcgctccgg tctgctgttg ccgcaagtcg
     4321 cgagcgagca caactgggac gtccccacct tcctcgagaa cacctgttac aaggcgggcc
     4381 tgccgggaga cgcgtggaag aaggcgggga ccaagatcga gcgcttctcg gcgctggtct
     4441 tcggtgagaa ggagctgggg ctgcccgctc gctgaggcgg cagtttactt aatcgcgtag
     4501 agcaccatgg agctggtgag cgcgaaatcg ttccagcgca ccgtgctttg cagatggtac
     4561 caggcgatgc gaaaccaggc gcggtaatcc gcgatgcttc gaaaaccgcc ccgtgatttc
     4621 agcagtttcg gcagcaggcc gaaatcgacc ggctgatacg cgacaattcg tgaaaagccc
     4681 gcacggcgaa gcgcgtcgct cagattgcgc ttggtgaaat acgagacgtg tcccggcagg
     4741 aagtaggcat agcgctccct gatccgacgg gcctggcgcg cgtcgaagtt cgccgtctgc
     4801 aataccagga gccctcccgg ccgcagcaat cgatggcatt cttcgatggc gaaaaccggg
     4861 tcggcgaggt gctcgatcac ctcgatcatc gttatcacgg aaaaataacc gctggggaac
     4921 gggtgatcgg cgagggttcc ctgatggagc gcgtcgccga gccagcgccg gcattcgccg
     4981 gcggcatagg gcgaaatctc gatgccgtac gggcggaatc gggactgtgc cgccttgaga
     5041 aatccaccga atgcgcagcc gacgtcgagc agcgcgccat cagacacgtg ccggcgtatt
     5101 gttttgagac gggcgttcca gacatgccgg gcgtggcgtt cggcggcgcg ctcgtcgcag
     5161 taggcgtatt cggcgctggc ggtgtagtat cccttgtcgt agaaggaggc gagcgccgcg
     5221 tcggtgaatc gcgggttcat gaagatgaag ccgcaccgac cgcagcggtc caccgtgaat
     5281 ggcgtcgaga accgatcgat cgcgtacagg aatgatatc
//
LOCUS       DAORHF010000251         5304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16068_length_5304_cov_5.538769, whole genome shotgun sequence.
ACCESSION   DAORHF010000251 DAORHF010000000
VERSION     DAORHF010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16068_length_5304_cov_5.538769"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="PKJ16_15835"
     CDS             complement(<1..335)
                     /locus_tag="PKJ16_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019646326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48514.1"
                     /translation="MTREHWGSKLGFVLAAAGSAIGLGNIWKFPYVAGANGGAAFVLV
                     YVLCVIVLGVPIMIAELVIGRHTESDPVGAFKLMFGRSGWVVIGYLGVVCGFLEAGNW
                     DGYHPHHKI"
     gene            complement(402..893)
                     /locus_tag="PKJ16_15840"
     CDS             complement(402..893)
                     /locus_tag="PKJ16_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48515.1"
                     /translation="MAMDVDRARRLFGLDERFGEDELAATYRILAKLNHPDVSARESS
                     DALMASINEAYALLRNVVRARTAAPGAVNDAAPAARAIDPVYEQYKKGFAALKAAFES
                     YFGETEDRRDEANLPLLRERLRAAKRDFSVVITDMPYSAWVDDAIDKVGAINKWLHER
                     DTR"
     gene            complement(910..1434)
                     /locus_tag="PKJ16_15845"
     CDS             complement(910..1434)
                     /locus_tag="PKJ16_15845"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HNV48516.1"
                     /translation="MFEEIFTRNRCTDFRWIEPSGIRVAHWVRMKCHYGCGGYGACAA
                     CPPETPSVAECREFFREYSRAALFHFRLRLDGPDERYAACRAINRQLLSVERDVFLAG
                     EERSFALFADNCSWCDECAGERRICRNKKDSRPTIEAMGVDVYSLARGAGYHIEVCVD
                     DRQAMDRFGLLLIR"
     gene            complement(1475..2560)
                     /gene="ychF"
                     /locus_tag="PKJ16_15850"
     CDS             complement(1475..2560)
                     /gene="ychF"
                     /locus_tag="PKJ16_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002931070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HNV48517.1"
                     /translation="MGLTCGIIGLPNVGKSTIFNALTGAGAAMANYPFCTIEPNHGIV
                     AVPDQRLRAIAELLKKTDPIPARIEFVDVAGLVAGASRGEGLGNKFLGNIRTVDAIVH
                     VVRCFDDENVVHVASGVDPVRDIEVIHTELVLADIEVLGRGLEKLRKIEKSGDKAVKA
                     KIPFLERVLAHLNEGRLLRAMTLESDEAAALGEYGLITGKPELLVANADDGGIDGPAA
                     EAVRAYAAASGAEYLAIAGKVEEEISELPAAEKAEFLGAMGIAESGLDRLIVVANRLL
                     GLITYYTAATHLQAWSIPIGTSARSAAGKIHSDFERGFIRAEVIHSDDLVRAGSEHHA
                     RERGLLRTEGKDYVVRDGDVVRYLFNV"
     gene            2666..3832
                     /locus_tag="PKJ16_15855"
     CDS             2666..3832
                     /locus_tag="PKJ16_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HNV48518.1"
                     /translation="MKRNEHAAPAPGSSPRIASLDAFRGLTIMLMLLVNNPGSWGDIY
                     PVLRHADWNGWTLADMVFPFFLWIVGFSIALSIGPRIAAGAPRASLMRKIFIRAAIIF
                     ALGLALNAFPFGLFGPPFDPGTLRIPGVLQRIALCYLGAALLTCFATPRRQLAIALVI
                     LAAYWYAMTHIPVPDVGTGVLEKGRNLAAHVDSLLLAGHLWPVTKTWDPEGILSTLPA
                     VATTILGAVAGSFMRGAPTPSLMRAMSLCSAGSIAAVSGIVAGYWFPINKSIWTSSYV
                     LLMAGLCMIVFAAAYSLIELTIAVRPFRPFIWCGRNPLTLFIASVLFAKTLSLLVIDG
                     GDGRMISLRDHVFRNHFLPLADPAGASLLYAIAFCGIFMLIGWLLWRRGIFIAL"
     gene            3899..>5304
                     /locus_tag="PKJ16_15860"
     CDS             3899..>5304
                     /locus_tag="PKJ16_15860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48519.1"
                     /translation="MSATAIILHSLLALAAFELIVMTHIRTRRLAAVFATSTLAASIP
                     LLIEIAALYGIVPAHVLTTARPALHAPFILSFGWLLNTYFLRHSAISIRALAAGSLAL
                     VVLSLALPGAIALQTVPLSLFALYAAAMLYGDITAEPNATAARDRLVVIGLLLAQTGA
                     LCANLVIEYEPLAAMSNVVLLALVSIRHFTGEFNRTAVMAHNVLISKDLNRQLVHSIG
                     RLSQKIEQLRQVISDKEAELLQLSKHASLAEITAGVAHELTQPLTGIKAIAQNMIDDI
                     TDDDVNTMEAVAELLKICAMVDKSSSIINHIRHFSGKSKIVRSGIDLNAVVLESIDLV
                     SYQFSKHAIDIVLDLPPEAAIIHGDAIALEQTIINLLLNARDAILEKRSIAPEDISGT
                     ITIRTTIDRSTVALAIEDNGTGIPPHTLANIWSPFFTTKKKSNGTGVGLSISRKIITE
                     HDGTVSVESTPDVGTRFI"
BASE COUNT          958 a         1870 c         1482 g          994 t
ORIGIN      
        1 ctaatcttgt gatgaggatg atatccatcc caattgcccg cctcgaggaa gccgcacacg
       61 accccgagat acccgatgac gacccagccg ctcctcccga acatgagctt gaaggcgccc
      121 accggatcgc tctcggtgtg tcggccgatc accagctcgg cgatcatgat ggggaccccc
      181 agaacgatca cgcacaggac atagaccagc acgaaggccg cgccgccgtt cgcaccggcc
      241 acgtacggga atttccagat attccccaat ccaatggccg accccgcggc ggccagaaca
      301 aatcccagct tcgatcccca gtgctccctc gtcatgctca tgctccttga caccgtgccc
      361 cgcggagtgc gccgcggacc accagactcc gcgatccacg gttatcgagt atcgcgctcg
      421 tgcaaccact tattgatggc gccgaccttg tcgatcgcgt cgtcgaccca ggcgctgtac
      481 ggcatgtccg tgatcaccac ggaaaaatcc cgcttcgccg ctcgcaggcg ctcacgcagg
      541 agcgggaggt tcgcctcgtc ccgccggtcc tccgtttcgc caaagtacga ttcgaacgcc
      601 gccttgagcg cggcaaagcc cttcttgtac tgctcataga ccggatcgat cgcgcgtgcg
      661 gccggcgcgg catcattgac ggcacccggc gcggcggtgc gcgcgcgcac gacgttccgc
      721 aggagcgcat aggcctcatt gatggacgcc atcaacgcat cggacgattc ccgcgcgctc
      781 acgtcgggat gattgagctt cgcgagaatt cgataggtcg ccgcaagctc gtcctcgcca
      841 aagcgctcgt ccagcccgaa gagccgtcgc gcgcgatcga catccatcgc catcagcgtc
      901 ccgcccgtat cacctgatca gcagaagacc gaaccggtcc atcgcctgcc ggtcgtcgac
      961 gcacacctcg atgtggtatc cggcgccgcg cgccagcgag tacacatcga cccccatcgc
     1021 ttcgattgtg ggcctcgaat cctttttatt tcggcagatc cggcgttcgc cggcgcattc
     1081 gtcacaccac gagcaattgt cggcgaacaa cgcaaaggac cgttcctcac cggcaagaaa
     1141 cacgtcccgc tcgacggaga ggagctgccg gttgatcgcc cggcaggcgg cgtatcgctc
     1201 gtcgggacca tcaagccgaa gccgaaaatg aaacaacgcc gcgcgggaat actcccggaa
     1261 aaactcgcga cactccgcga cagacggcgt ttccggagga caggccgcgc atgcgccgta
     1321 cccgccgcac ccatagtggc atttcatccg aacccagtgc gcaacgcgga taccagacgg
     1381 ctcgatccat cggaaatcag tgcagcgatt ccgagtgaat atttcttcga acatgctttc
     1441 catcggcact tcccgcatcg atgcaaccgt tccgctagac attaaacaga taccgcacta
     1501 cgtcgccgtc gcggaccaca tagtccttgc cctcggttcg aagcagcccc cgctcgcgcg
     1561 cgtggtgctc cgatccagcg cgcaccaggt cgtcactgtg gatcacctcg gcgcggataa
     1621 agccgcgctc gaaatcggag tggatctttc ccgctgcgga gcgcgcgctg gtgccgatcg
     1681 gtatcgacca tgcctgcagg tgggtcgcgg cggtataata ggtgattagc cccagcagcc
     1741 ggttggcgac gacgatgaga cgatcgagcc ccgattccgc gatccccatc gcgcccaaaa
     1801 actccgcctt ctcggccgcc ggcagctccg agatctcctc ctccaccttt ccggcgatgg
     1861 cgaggtactc ggcgcctgag gcggcggcat atgcccgcac cgcctcggcg gccggaccgt
     1921 cgatcccgcc gtcatcggca ttggccacca atagttccgg tttaccggtt atcaggccgt
     1981 actcgccgag ggctgcggcc tcgtcggatt cgagcgtcat ggcgcgcagc aaccgccctt
     2041 cgttcagatg cgccagaacg cgctccagaa acgggatttt cgccttcacg gccttgtcgc
     2101 ccgatttctc gatcttgcgc agcttctcga gcccgcgccc cagcacctcg atgtcggcca
     2161 ggacgagctc ggtgtggatc acctcgatat cgcgcaccgg gtccacgccg ctcgcgacat
     2221 gcacgacgtt ttcgtcatcg aaacagcgca ccacgtggac gatcgcgtcg accgtgcgga
     2281 tgttcccgag gaacttgttc cccagcccct cgccgcggga ggcgccggcc accagcccgg
     2341 ccacgtccac gaactcgatg cgggccggaa tcggatcggt ctttttcagt agttcggcga
     2401 tggcgcgaag ccgctggtcg ggaacggcca cgatgccgtg gtttggctcg atggtgcaga
     2461 agggatagtt cgccatcgcg gcgcccgcgc cggtgagcgc attgaagatg gtcgatttcc
     2521 cgacattggg caggccgatg ataccgcagg tgagtcccat cgcgcgctcc tttagaagtg
     2581 tgaatgcacc acatccggcg cgttcgatat tagcaagaaa tattttcctt tgacgcgcca
     2641 tgccccgtcc gctagcgttg gccggatgaa acgaaacgaa cacgccgcac ccgcccccgg
     2701 atcatcgccg cgcatcgcga gcctcgacgc cttccggggc cttacgatca tgctcatgct
     2761 cctggtgaac aaccccggct cctggggcga catctatccc gtcctgcgtc acgcggactg
     2821 gaacggctgg acgctcgcgg atatggtgtt tccgtttttt ctctggattg tcgggttttc
     2881 catcgcgctg tcgatcgggc cccgaatcgc ggcgggggcc ccccgtgcga gcctaatgag
     2941 aaaaattttc atccgcgccg cgatcatctt cgcgctgggg ctcgccctga acgccttccc
     3001 gttcggcctc ttcggccccc cgttcgatcc cggtacgctg cgcataccgg gcgtcctgca
     3061 acggattgcc ctctgctatc tgggcgccgc gctcctcacg tgcttcgcga ccccgcgccg
     3121 gcagctcgcg atcgcgctcg tcatactcgc cgcctactgg tacgcgatga cgcacatacc
     3181 cgtgcccgac gtcggaaccg gcgttctcga gaagggccgt aatcttgccg cgcatgtcga
     3241 ttccctcctg ctcgcggggc atctctggcc ggtcacgaag acctgggacc ccgaaggcat
     3301 cctgagcacc ctgccggcgg tcgcgaccac gatactcggc gccgttgccg gatccttcat
     3361 gcgtggcgca ccgacaccct cgctgatgcg cgcgatgtct ctgtgcagtg ccggcagtat
     3421 cgccgctgtt tccggtatcg ttgcgggcta ctggtttccc ataaacaaaa gcatctggac
     3481 cagctcatac gtcctcctca tggccgggct gtgcatgatc gtgttcgccg cggcgtactc
     3541 actcatcgag ctcacaatcg ccgtccgccc gtttcggccg ttcatctggt gcggacgcaa
     3601 tccgttgacg ctcttcatcg cctcggtgct cttcgcaaaa acgctctccc tgctcgtcat
     3661 cgacggcggc gacggaagga tgatctccct tcgggaccac gtgttccgaa accactttct
     3721 cccgctcgcc gatcccgcgg gcgcatcgct cctctacgcg atcgccttct gcggcatctt
     3781 catgctgatc ggctggctgc tctggcgccg gggcatcttc attgcgttgt gaccgccaaa
     3841 agcgtttgac agattcccgc gcccggaaag gattgtcgca atcacagata cacacgccat
     3901 gagcgccacc gccatcatac tccactcgct cctcgcgctt gccgcgttcg agctcatcgt
     3961 catgacgcac attcgcaccc ggcggctcgc ggcggtcttc gcgacaagca cgcttgccgc
     4021 gtcgatcccg ctcctcatcg agatcgcggc gctgtacggc atcgtacccg cgcatgtcct
     4081 cacgaccgct cgtccggcgc tccatgctcc gttcatcctc tcgttcggct ggctgctcaa
     4141 tacgtatttt ttacggcaca gcgccatcag catacgcgcg ctcgccgcgg gatccctggc
     4201 gcttgtcgtg ttatcgctcg cgcttcccgg cgccatcgcg ctccaaacgg ttcccctgtc
     4261 gctctttgcc ctgtacgctg cggccatgct ctatggcgac atcacggccg aaccgaacgc
     4321 gacggcagcc cgcgaccgcc tggtcgtcat cggcctgctg ctcgcccaga cgggcgcgct
     4381 gtgcgcgaac ctggtgatcg agtacgagcc gctcgccgcg atgtcgaacg tcgtgctgct
     4441 cgcgctcgtc agcatccggc acttcaccgg cgaattcaac cgcacggccg tcatggcgca
     4501 taacgtctta atctccaaag atctcaatcg ccagctcgtg cacagcatcg gccgcctctc
     4561 gcagaagatc gagcagctgc gccaggtcat cagcgacaag gaggcggagc tgctccagct
     4621 ctcgaagcac gcgagcctgg cggagatcac cgcgggggtc gcgcacgagc tcacgcagcc
     4681 gctcaccggg atcaaggcca tcgcccagaa catgatcgac gacatcaccg acgacgacgt
     4741 gaacaccatg gaggcggtgg cggagctcct caagatatgc gcgatggtcg acaagtcctc
     4801 gtcaatcatc aaccacatcc gtcatttctc ggggaaaagc aagatcgtcc ggtccggcat
     4861 cgacctgaac gcggtcgtcc tcgaatcgat cgatctcgtc tcctaccagt tcagcaagca
     4921 tgcgatcgac atcgtactcg accttccgcc ggaggcggcg atcattcacg gcgacgcaat
     4981 cgccctcgag cagaccatca tcaacctgct cctcaacgcg cgcgatgcga ttctcgaaaa
     5041 gcgctccatc gcgccggagg atatttcggg aacgattacc atccgcacaa ccatcgaccg
     5101 ctcgacggtc gcgctcgcca tcgaggacaa tggcaccggg atcccgccgc acacgctcgc
     5161 gaacatctgg tcgccgtttt tcaccaccaa gaagaagtcg aacggcaccg gcgtcgggct
     5221 atcaatcagc agaaaaatca tcaccgagca tgacgggacc gtctcggtcg aatccacgcc
     5281 cgacgtgggt acccgcttca tcat
//
LOCUS       DAORHF010000252         5295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16109_length_5295_cov_4.359924, whole genome shotgun sequence.
ACCESSION   DAORHF010000252 DAORHF010000000
VERSION     DAORHF010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16109_length_5295_cov_4.359924"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /gene="rpsC"
                     /locus_tag="PKJ16_15865"
     CDS             <1..442
                     /gene="rpsC"
                     /locus_tag="PKJ16_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HNV48520.1"
                     /translation="IGRKGAGIEVLKNDLQKIASKTVYINIVEVKKPERVSKLIAEQI
                     ASQLVGRFPYRRAVKQAISSAIRGGVLGIKVAVSGRLNGAEISRSESYKEGRIPLHTL
                     RADIDFGFAEALTTFGLIGVKVWVYNGDILSSDANKEEDRYAVK"
     gene            494..901
                     /gene="rplP"
                     /locus_tag="PKJ16_15870"
     CDS             494..901
                     /gene="rplP"
                     /locus_tag="PKJ16_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HNV48521.1"
                     /translation="MLMPKRTKYRKLQRGRMKGKAQRGSTVAFGEVGLKALECGKISN
                     RQIEAARVAITRKIKRGGKLWIRIFPDYPFTKKPAETRMGKGKGNPEGWVARIKPGRI
                     LFELAGVEEPLAKEAFRLAAFKLPVKTKVVSIK"
     gene            927..1136
                     /gene="rpmC"
                     /locus_tag="PKJ16_15875"
     CDS             927..1136
                     /gene="rpmC"
                     /locus_tag="PKJ16_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HNV48522.1"
                     /translation="MKGKIEDMSIEELGRSLKETKEELRKERFKSVTSKLENPQKIKA
                     LRRHVARVNTIMREYELGIRNLKGK"
     gene            1178..1426
                     /gene="rpsQ"
                     /locus_tag="PKJ16_15880"
     CDS             1178..1426
                     /gene="rpsQ"
                     /locus_tag="PKJ16_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HNV48523.1"
                     /translation="MGRKKQLVGKVVSNKMDKTIVVKIERMMMHPLYNKPVQFSKKVK
                     GHDEKNECSIGDTVRIEETRPLSRDKRYRLIAIVEKVK"
     gene            1463..1855
                     /gene="rplN"
                     /locus_tag="PKJ16_15885"
     CDS             1463..1855
                     /gene="rplN"
                     /locus_tag="PKJ16_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HNV48524.1"
                     /translation="MIQVETFLDVADNSGVKRVQCIKILGGTRRRYASAGDIIVVAVK
                     DSHPSYGLKDSTGKKVHGKAVLRAVVVRTKKPVRRKDGSYIRFDDNAVAIINTKNEPI
                     GSRIFGPVARELREKNFLKIVSLAPEVL"
     gene            1865..2206
                     /gene="rplX"
                     /locus_tag="PKJ16_15890"
     CDS             1865..2206
                     /gene="rplX"
                     /locus_tag="PKJ16_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HNV48525.1"
                     /translation="MKKSVKKGFVRSVVKKDDSVVVTAGADRGKRGKVLFVDRSRGRI
                     VVEGINKKKKFVRPSQQNPKGGAISLEFPINISNVLLFCEKCKKGVRVSMRMKGEKKV
                     RSCSACERNFD"
     gene            2253..2801
                     /gene="rplE"
                     /locus_tag="PKJ16_15895"
     CDS             2253..2801
                     /gene="rplE"
                     /locus_tag="PKJ16_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HNV48526.1"
                     /translation="MSARLREDYQKSIRKRLFEKMKYSSVMQVPRLEKIVLNVGMGDA
                     HSNATALTAIIDELSTITGQKAVKTVAKKSIANFKIRDGYEVGARVTLRGNIMFEFLD
                     RLINVSLPRVRDFRGLSPKSFDNFGNYNFSIREQIIFPEIDFDKVDKIHGINVTIVTT
                     GKSSEEARALLDEFGFPFREQG"
     gene            2808..2993
                     /locus_tag="PKJ16_15900"
     CDS             2808..2993
                     /locus_tag="PKJ16_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HNV48527.1"
                     /translation="MASTCHIAKSNSPRKFKVRQHNRCKICGRPRAYLRRFEMCRICF
                     RNLANAGKLPGVVKSSW"
     gene            3018..3419
                     /gene="rpsH"
                     /locus_tag="PKJ16_15905"
     CDS             3018..3419
                     /gene="rpsH"
                     /locus_tag="PKJ16_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HNV48528.1"
                     /translation="MGKYNDPIADMLTRIRNAIKAGHLKVDIPSSKMKAAIARILKDE
                     GYIKNYKVIDDKKQRILRVYLKYTPDNASAIYSLSRISKPGRRIYVQAESLRPVYNNI
                     GIRILSTSKGIVTNKAAKQLNIGGEVLCEIS"
     gene            3441..3983
                     /gene="rplF"
                     /locus_tag="PKJ16_15910"
     CDS             3441..3983
                     /gene="rplF"
                     /locus_tag="PKJ16_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HNV48529.1"
                     /translation="MSRIGNKPLQIPGGVTVEVSNNVISVKGPLGSDSQAIHASIGVT
                     QKDGTLLVEKLGGADDKATRASHGLYRSLIGNMLTGVSKGFERELEIVGVGYRAQQQS
                     KDISFQLGYSHAIVFPAPDGITLEVVEPTRIRVKGVNKQQVGQVAANIRELRPPEPYK
                     GKGIKYKDEYIRRKAGKTGA"
     gene            3986..4351
                     /gene="rplR"
                     /locus_tag="PKJ16_15915"
     CDS             3986..4351
                     /gene="rplR"
                     /locus_tag="PKJ16_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HNV48530.1"
                     /translation="MNKVDQKKIRLRRRKLRIKRKLKETSERPRMCISRSNKHFRVQI
                     IDDRAGVTLVAASTLDKEFPEMKNRGNITAAQALGKLIAEKAKQRGVTTIVFDRNGML
                     YHGKIKAFADAARESGLTF"
     gene            4364..4876
                     /gene="rpsE"
                     /locus_tag="PKJ16_15920"
     CDS             4364..4876
                     /gene="rpsE"
                     /locus_tag="PKJ16_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HNV48531.1"
                     /translation="MKIKRKTVNPEGFELTEKVVLIKRVAKVVKGGRRFSFSALSVVG
                     DENGHIGIGFGKANEVPDAIRKSVEKAKKNVYQVNVNKNTIPHQVIGSFKSARVLLKP
                     AVPGTGIIAGASVRALVEKAGIKDVLAKSQGSRNPMNIVKATLDGLLQLRTLKEVADV
                     REVTLKKIMN"
     gene            4890..5078
                     /gene="rpmD"
                     /locus_tag="PKJ16_15925"
     CDS             4890..5078
                     /gene="rpmD"
                     /locus_tag="PKJ16_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HNV48532.1"
                     /translation="MAKKLIIKQIRSGIGAKPKQRATLRALGLRRMQRERTHDDTAVI
                     RGMINTVQHLVRVTEVKE"
     gene            5102..>5295
                     /gene="rplO"
                     /locus_tag="PKJ16_15930"
     CDS             5102..>5295
                     /gene="rplO"
                     /locus_tag="PKJ16_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HNV48533.1"
                     /translation="MSESYRIQRPANLKTRKRVGRGTSSGSGKTSARGQKGQMSRSGC
                     NRRAWFEGGQMPIQRRVPKRG"
BASE COUNT         1359 a         1301 c         1670 g          965 t
ORIGIN      
        1 tatcggtcgc aagggtgccg gcatcgaggt gctgaaaaac gatcttcaga agatcgcttc
       61 gaagaccgtg tatatcaaca tcgtggaagt gaaaaagccg gagcgcgtct ccaagctcat
      121 cgcagagcag atcgcttcgc agctcgttgg gcgcttcccc tatcgtcgcg cggtgaagca
      181 ggcgatttcg tcggcaatac gcggcggggt gctggggata aaggtcgcag tgtccggacg
      241 cttgaatgga gccgagattt cgcgttcaga atcgtacaaa gagggacgga ttccgctgca
      301 caccctgcgc gccgacatcg atttcggatt tgccgaggcg ctgacaacct tcggcctcat
      361 cggcgtgaag gtttgggtgt ataacggcga cattctctcg tcagatgcga acaaggaaga
      421 ggaccgatac gcggtcaagt agcataatcg aatcacggaa agcatcgaat acgttaagta
      481 agggataatt accatgttaa tgcccaaacg gacaaaatac cgcaagctgc agcgggggcg
      541 catgaagggc aaggcccagc gcgggtcgac ggtcgcattt ggcgaggttg ggctcaaggc
      601 gctcgagtgc gggaagatct ccaaccggca gatcgaggcg gcgcgtgtcg cgattacccg
      661 caagatcaag cggggcggca agctgtggat ccgcattttt ccggattatc cctttaccaa
      721 gaagccggcc gaaacccgga tggggaaggg gaaaggcaat cccgaggggt gggtcgcgcg
      781 cattaagccg ggccgcattc tcttcgagct cgccggcgtg gaagagccgt tggccaagga
      841 ggcgtttcgc cttgccgcct tcaagcttcc cgtcaagacc aaggttgtat ccattaagta
      901 gttaactgaa tttagggata ggcatcatga aaggcaaaat tgaagatatg agcatcgagg
      961 agctgggccg cagcctcaag gaaacgaagg aggagttgcg gaaagagcgg ttcaagtcgg
     1021 ttaccagcaa gctcgaaaat ccccagaaga taaaggcgct ccgacggcat gttgcccgag
     1081 tcaacaccat catgcgggag tatgagctcg gcattcgcaa tctgaaggga aagtagcccg
     1141 gcggagcagg gcaatcattg gatgacaggg taaggcaatg ggtcggaaga aacagctagt
     1201 cggaaaggtc gtcagcaata aaatggacaa gaccatcgtc gtcaagatcg aacggatgat
     1261 gatgcatccg ttgtacaaca agcccgtcca gttttcgaag aaggtgaagg ggcacgacga
     1321 gaaaaacgag tgcagcatcg gggacacggt gcgcatcgag gaaacgcgtc cgctctcgcg
     1381 ggataagcgc taccggctaa tcgcgattgt cgagaaggtc aagtaggcac cgggtgccgc
     1441 agggcgataa ggagttcgag cgatgataca ggttgaaaca tttcttgatg tggccgataa
     1501 cagcggcgtc aagcgggtgc agtgcatcaa gattctcggc ggaacccggc gtcggtacgc
     1561 atcggcgggg gacattatcg tggttgccgt gaaggactcg cacccctcgt acggtctgaa
     1621 ggattccacc gggaagaagg ttcacggcaa ggcggtgttg cgggccgtgg tggtgagaac
     1681 gaaaaagccg gtgcgccgca aggacggatc gtatattcga tttgacgata acgccgtcgc
     1741 gatcatcaac accaagaacg agccgatcgg ttcgcgtatc ttcgggccgg ttgcgcgcga
     1801 gctcagggag aagaactttc tgaagatcgt atcgctcgcg ccggaagtgc tgtaagggga
     1861 aatcatgaaa aagagcgtca aaaagggctt cgtgagaagc gttgtcaaaa aagacgatag
     1921 cgtcgtggta acggcgggcg ccgatcgcgg caaacgagga aaggttctgt tcgtggatcg
     1981 aagtcgcggg cggatcgttg tcgaagggat caacaagaag aagaagttcg ttcgcccctc
     2041 acagcagaat cccaagggcg gcgccatcag cctcgagttt cccatcaaca tctccaatgt
     2101 gttgctgttc tgcgagaagt gcaaaaaggg cgtgcgcgtg agcatgcgga tgaagggcga
     2161 aaaaaaggtc cggtcgtgca gcgcgtgtga gaggaatttc gactaatgcc ggacgcgcga
     2221 tgcgatcggg tatagcagcg taggtgagga acatgtcggc aagattgcgt gaagattatc
     2281 agaagagcat ccggaagcgg ctgttcgaga aaatgaagta ctcctcggtg atgcaggtcc
     2341 cgcggcttga gaaaatcgtc ctcaatgtcg ggatgggcga tgcgcactcg aatgcgaccg
     2401 cgcttacggc catcatcgac gagctgtcca ccatcaccgg gcaaaaggct gtcaagacgg
     2461 tcgccaagaa gtccatcgcg aacttcaaga tccgcgacgg atacgaggtt ggcgcccgcg
     2521 tgacgctgcg cggtaatatc atgttcgagt ttctcgatcg gctgatcaat gtgtcgctgc
     2581 cccgggtccg cgacttccgg gggttgtccc cgaaatcgtt cgataacttt ggaaattaca
     2641 atttctcgat acgggagcag attatctttc cggaaatcga cttcgacaag gtggacaaga
     2701 tccacggcat caacgtgacg attgtcacga ccgggaagag cagcgaggaa gcgcgcgcgc
     2761 tcctcgatga gttcggcttt ccattcaggg aacaggggtg atagaacatg gcaagcacgt
     2821 gccacattgc gaaaagcaac tcgccgagaa aattcaaggt gcgccagcac aatcgctgca
     2881 agatttgcgg tcggccacgc gcctatctgc gtcgctttga gatgtgccgg atctgctttc
     2941 gaaacctggc gaacgccggg aagctgccgg gcgtcgtgaa atcatcatgg tgaatcagta
     3001 cgagggagta gtgtattatg ggtaagtata acgatccgat cgccgatatg ctcacgagga
     3061 tccggaacgc cattaaggcg ggccatctca aggtggacat tccctcgtcg aaaatgaagg
     3121 cggcaatcgc gcgcatcctc aaagacgagg ggtacatcaa gaactacaag gtgattgacg
     3181 acaagaaaca gcgcattctg cgcgtgtatc tcaaatatac cccggacaac gcctcggcga
     3241 tttattcgct gtcgcgcatc agcaagccgg gacgccgcat atacgtacag gcggagagcc
     3301 tgcgtccggt gtataacaat atcgggattc gaatactgtc gacgtcgaag gggattgtta
     3361 caaacaaagc ggcgaaacag ctcaacattg gcggcgaagt gttgtgcgag atatcataac
     3421 gacgaagcga gcgaagagca atgtcacgaa ttggaaataa gccactgcag atacccggcg
     3481 gggtcaccgt tgaggtttcg aacaatgtaa tcagcgtgaa gggcccgctg gggagcgatt
     3541 cccaggcgat ccacgccagt atcggcgtga cgcagaagga cgggacgctt cttgtggaaa
     3601 agcttggcgg tgcggacgat aaggcgaccc gcgcctccca cggactgtat cgttcgctca
     3661 tcgggaacat gctcaccggc gtttcaaagg ggttcgagcg agagcttgag atcgttgggg
     3721 tcggctatcg cgcccagcag cagagcaagg atatttcttt ccagcttggg tattcgcatg
     3781 ccatcgtctt ccccgcgccg gacggtatca ccctcgaggt ggttgagccg acccgcatac
     3841 gggttaaagg cgtaaacaag cagcaggttg ggcaggttgc ggcgaatata cgcgagctgc
     3901 gtccgccgga accgtataag ggcaagggaa tcaagtacaa ggacgaatat atcagacgga
     3961 aagctggtaa gacgggtgca tagcaatgaa taaggttgac cagaagaaga tacggcttcg
     4021 gcgtcgaaag cttcgaatta agcgcaagct gaaagagacc tccgagcggc cgcggatgtg
     4081 tatttcgaga agcaacaagc attttcgggt ccagatcatc gacgatcgtg cgggcgtgac
     4141 gctcgttgcc gcgtcgacac tcgacaagga attcccagaa atgaagaacc gtggaaacat
     4201 cacggcggcg caggcgctgg gtaaactgat tgccgaaaaa gcgaagcagc gcggtgtgac
     4261 gaccattgta ttcgaccgca acggaatgct gtatcacgga aagattaagg ccttcgccga
     4321 tgcggcgcgc gaaagcggct taacgtttta ggagagcgga ctgatgaaga tcaaacggaa
     4381 aaccgtcaat cccgagggat tcgaactcac cgagaaggtt gtgctgataa agcgcgtcgc
     4441 gaaggtcgtg aagggcggtc gtcgcttctc atttagcgcc ctctccgtgg tcggtgacga
     4501 aaacggccat atcgggatcg gtttcgggaa agcgaacgaa gtacccgatg cgatccggaa
     4561 aagcgtggaa aaggcgaaaa agaacgtgta ccaggtgaac gtgaacaaga acacgatccc
     4621 gcatcaggtg atcggttcgt tcaagtcggc gcgggtgctc ctgaaaccgg ccgttcccgg
     4681 aaccggcatc atcgccggcg catccgttcg ggcgctcgtc gaaaaggccg ggatcaagga
     4741 cgtgcttgcc aaatcgcagg gttcacgcaa tccgatgaat atcgtaaagg ccaccctgga
     4801 cggattgctt caactgagaa cgctcaagga ggtggccgac gttcgcgagg tcactctcaa
     4861 gaagataatg aactaatacg ggttttcaca tggcgaagaa actgatcatc aagcagatac
     4921 gaagcggcat cggcgcaaag ccgaagcagc gagcgacgtt gagggcgctc ggactgcgcc
     4981 gcatgcagcg cgaacgcacg cacgatgaca cggccgtaat caggggaatg atcaatacgg
     5041 tgcagcatct tgtgcgcgtt acggaagtga aggaatagtc agaacggatg gagatcgatc
     5101 gatgagcgaa tcctatcgta tacaacggcc ggcaaacctc aaaacgcgca agcgggttgg
     5161 gcgcggcacc agcagcggat cgggaaaaac gtccgcacgc gggcagaagg ggcagatgag
     5221 ccgttcgggg tgcaaccgcc gtgcatggtt tgaaggcggt cagatgccca tccagcgccg
     5281 ggtcccgaag cgcgg
//
LOCUS       DAORHF010000253         5270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16235_length_5270_cov_4.442378, whole genome shotgun sequence.
ACCESSION   DAORHF010000253 DAORHF010000000
VERSION     DAORHF010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16235_length_5270_cov_4.442378"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            272..841
                     /locus_tag="PKJ16_15935"
     CDS             272..841
                     /locus_tag="PKJ16_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48534.1"
                     /translation="MGIKRKQYVLDKAFQLGITLKAVVVPLVTILIFSGVLIYFANQN
                     NTYIRNNDDVIKEIVGTQESMIEMFLRTPALYKSENEAVRNGQRTFEDNIGKLMKINK
                     TSRLIVRNSSLVLYALILMTVVQTAIIFILFIYFTHKISGPMHVMTNHLRAIRQGKKP
                     HFRPLRKRDEFQAFYIELKETIDYLSEKK"
     gene            866..1063
                     /locus_tag="PKJ16_15940"
     CDS             866..1063
                     /locus_tag="PKJ16_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48535.1"
                     /translation="MLRGPMKASELTCLYCGSGLELKFEHLVIGENSGVCPMCAEPYC
                     IKLNQDDMAGFIDAEEIRPSH"
     gene            complement(1077..1562)
                     /locus_tag="PKJ16_15945"
     CDS             complement(1077..1562)
                     /locus_tag="PKJ16_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HNV48536.1"
                     /translation="MTDYLFNFDKLAYVKGDKPRVGCILCAIRDRDSRVTMLDAYRGE
                     GMIVSLNLYPFNPGHLMIFPERHVAEYGDLQDGEALAMHRLTAHAIAILREEFNPTGF
                     NVGYNIGENSGASIPHLHQHVVPRYGNEVGFLDVLAGARVWVADPQETLRRLRDRFSG
                     A"
     gene            complement(1574..2413)
                     /locus_tag="PKJ16_15950"
     CDS             complement(1574..2413)
                     /locus_tag="PKJ16_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48537.1"
                     /translation="MNYRICIPAFVLAFAMIVPIAIFADAPNAARDADGCRACHQDLK
                     GKLQAPVGPWKGSAHARDGRMCTSCHGGRADAADSRSAKDPKTNFIGKTDKRATTEFC
                     GRDGCHANVVAYFRAGRHAGSVKKAGEPNCVSCHGSHHIERSSARIMTESRCVPCHTP
                     AFAKGVFASLKDADQLTSPIEADIEFLKKKSADTKDLDGKMASLRDHRTRMLHTLTRE
                     SMSMARGLIENEQAILKDVVRTKIAIVKRLDLIYQIIMIFCMCLILGFGVYTFRVYRR
                     KAE"
     gene            complement(2448..2924)
                     /locus_tag="PKJ16_15955"
     CDS             complement(2448..2924)
                     /locus_tag="PKJ16_15955"
                     /inference="COORDINATES: protein motif:HMM:NF016489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HNV48538.1"
                     /translation="MILKEILFTFFYSGYFPIAAGTAGSVVAMGVYVLGYVLFGERVW
                     IANLVLVALLFFPAIRIGDWGERYFGDKDPSPVVLDEAMGYWITVLFHPFSWSVVFLG
                     FVIFRIMDIIKPFPARRLERLPGGRGIMIDDYIAGAYSNIVLIVIVFASRWLNCPI"
     gene            complement(2921..3580)
                     /gene="pgsA"
                     /locus_tag="PKJ16_15960"
     CDS             complement(2921..3580)
                     /gene="pgsA"
                     /locus_tag="PKJ16_15960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HNV48539.1"
                     /translation="MNIPNLLSALRIVLIPLFLYLLFVPSFSSRIWALVVFAFASLTD
                     LVDGWSARKLNQETSFGKFLDPLADKFLVIAALVAFVILDPLVSLWMVFVIFGRDLMI
                     TLMRYLAIKKGEELRTSQFGKIKTAFQMISIIVIIMVFIVRSSGIDVASPFIDKSHID
                     IVSACDVWQSGHPNRLLIIGPYVLMMIVTVLTALSGVRYLFTNWRLLLPPYVKRDQDG
                     R"
     gene            complement(3577..4872)
                     /gene="rimO"
                     /locus_tag="PKJ16_15965"
     CDS             complement(3577..4872)
                     /gene="rimO"
                     /locus_tag="PKJ16_15965"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HNV48540.1"
                     /translation="MSRPSPRARTFSILSLGCSKNQVDSERVNGALASAGLVPVTGDD
                     ADIVIINTCGFIEDAKTESIDAIFEAIRSRCGGQKIVVMGCLAQRYLDDMRREIPEID
                     FIAGIPDDAMVARMLRAIGVRAASAPVYRQCPLVPGEPHAYIKISEGCSNNCSYCAIP
                     LIRGPHVSDTPARIVDEARRAVQRGARELVVVGQDIARYRYGRTRLPELVERMARING
                     VRWIRLMYLHPDHVDDAIIDLVATNNSVVKYLDIPLQHASDEILRAMGRGGTGARYRE
                     LIETLRDRVPGIRIRSTFMVGFPGETDRHFRELVTFVRRVAIDRAGCFVYSPEEGTDA
                     ASRRGRVPKAVGRRRFDELMKVQQAISKRRLRAMIGERVEVLVEEHLEGDVWVGRTQY
                     DAPEVDGIFYLTGPGIERHAIVAARVTDATEYDLMGVPL"
     gene            complement(4928..>5270)
                     /locus_tag="PKJ16_15970"
     CDS             complement(4928..>5270)
                     /locus_tag="PKJ16_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HNV48541.1"
                     /translation="DKGQVVGDHTKVTTQSEKNLRIVFEVEFNKQTYLDLYLDGVNKR
                     KGLVQPGTREKWEAVEYIQVKIGNAGGVTARINGKPHVFGLPGQVANKVITWRKDSKN
                     PNEYKIVVKDW"
BASE COUNT         1121 a         1651 c         1517 g          981 t
ORIGIN      
        1 cacctggtcg cgccagtcct tgatgagcac gaacagcacc gagaacagca agagcaccga
       61 cgcgacgagc gaggagatga tactaacggt ttgcatgggc cgcccccagg gagcgcacga
      121 cgcgctccgt tggataaatg ccgaaaaaat tcattgacgt ttttgcctca gtaccgcctc
      181 atttgagcat tccgattacg ggcgtaccgg gtatcgaccg tacaacatcg tccgttttat
      241 tgcaataaca attcctgttc cgaggtgacc catgggaatc aagcgcaaac aatacgtact
      301 cgacaaggcc ttccagctcg gcatcaccct gaaggccgtc gtcgttccgc tggtcaccat
      361 cctgattttt tccggcgttc tcatctattt cgcgaaccag aacaacacct acattcgaaa
      421 caacgacgac gttatcaagg aaatcgtggg cacccaggaa agcatgatcg agatgttcct
      481 gcggaccccc gccctgtaca aatcggaaaa cgaggcggtc cgcaacggcc agaggacctt
      541 cgaggacaac atcggcaagc tcatgaagat caacaagacc agccgcctca tcgtccgcaa
      601 cagcagcctg gtgctctacg ccctcatcct catgaccgtc gtccagaccg ccatcatatt
      661 cattctgttc atttacttca cgcataagat ctccggcccc atgcatgtga tgaccaacca
      721 cctcagggcc atccgtcagg ggaaaaagcc ccacttccgc cctctgcgga aacgggacga
      781 gttccaggcc ttctacatcg agcttaagga aacgatcgat tatctgagcg agaagaagta
      841 ggcgccgggg ctacgggagg atatcatgct gcgcggaccg atgaaggcga gcgaactcac
      901 ctgcctgtac tgcggatcgg gccttgagtt gaaattcgag cacctggtga tcggcgagaa
      961 ctccggcgtt tgccccatgt gcgccgaacc gtactgcatc aaactcaatc aagacgacat
     1021 ggccggcttc atcgacgccg aggaaatccg gccgtcgcac tgatcgcgcc gcgcgcctac
     1081 gctccggaaa accgatcgcg caaccgccgc agcgtctcct gaggatcggc gacccagacg
     1141 cgggccccgg cgagcacatc cagaaagccc acctcgtttc cgtagcgcgg caccacatgc
     1201 tgatgcaggt gcggaatgct cgcgccgctg ttctcgccga tattatagcc gacattgaac
     1261 ccggtcggat tgaactcctc gcgcagaatg gcgatggcgt gcgcggtgag gcgatgcatc
     1321 gccagcgcct caccgtcctg caggtccccg tattccgcga catgccgctc cgggaagatc
     1381 atcaaatggc cgggattgaa cggatagagg ttgagggata cgatcatgcc ctcgccgcga
     1441 tacgcgtcga gcatcgtaac ccgcgaatcg cgatcgcgga tcgcgcatag gatgcacccg
     1501 acgcgcggct tgtccccctt gacgtatgcg agcttgtcga aattgaacag gtagtccgtc
     1561 atgtcgcgcg gacttattcg gccttgcgcc gatagacgcg gaacgtgtac acgccaaagc
     1621 ccaggatgag gcacatgcaa aagatcatga tgatctggta gatgagatcg agacgcttca
     1681 cgatcgcgat cttggtccga acgacgtcct tcagtatcgc ctgctcgttt tcgatgagcc
     1741 cgcgcgccat cgacatcgac tcccgggtga gcgtgtgcag catgcgggtg cgatgatcgc
     1801 gcaatgacgc catcttcccg tccaggtcct tcgtgtcggc gctctttttt ttcagaaact
     1861 ctatatcggc ctcgatcggc gaggtgagct gatccgcgtc cttcagcgag gcaaagaccc
     1921 ctttcgcgaa tgccggggta tggcagggaa cgcatcgcga ttcggtcatg atccgtgcgg
     1981 atgaacgctc gatgtgatgg gaaccgtggc acgatacgca attcggctcg ccggccttct
     2041 tgacgctccc ggcgtgtcga ccggccctga aatatgcaac gacgttcgca tgacagccgt
     2101 cgcgcccgca aaactcggtt gttgcgcgct tgtcggtttt tccgataaag ttcgtcttcg
     2161 ggtccttggc gctgcgcgag tcggcagcat cggcgcgccc cccatgacag gaggtgcaca
     2221 tgcgtccgtc gcgggcgtgc gcgcttccct tccacggccc gaccggcgcc tgcagcttac
     2281 ccttcaggtc ctgatgacac gcccggcagc cgtcggcgtc tcgagccgcg ttcggcgcgt
     2341 ctgcgaaaat cgcgatcggg acaatcatgg cgaatgcgag gacgaatgcc gggatgcaga
     2401 tgcgatagtt cattgccggt atctccgatg gaaaatctct actgtatcta tatcggacaa
     2461 ttcagccacc gactcgcaaa aacaatcaca ataagcacga tgttggaata cgcgccggcg
     2521 atgtaatcgt ctatcatgat gccccggcct cccggcagcc gctcaaggcg ccgcgccggg
     2581 aagggcttga tgatgtccat gatccgaaaa atcacaaacc ccagaaaaac gacgctccag
     2641 ctgaacggat ggaacaggac cgtgatccag taccccatgg cctcgtcgag aaccacgggc
     2701 gacgggtcct tgtcgccgaa atagcgctcg ccccagtccc cgatgcgaat cgccggaaaa
     2761 aacaggagcg cgacaagcac caggttggcg atccatacgc gctcgccgaa caggacatac
     2821 ccgagcacgt acacgcccat cgccacaacg ctgccggccg tgccggcggc gatcggaaaa
     2881 tagcccgagt agaaaaacgt aaagaggatt tccttcaaga tcatcgaccg tcctggtccc
     2941 gtttcacata gggcggcagc aacagccgcc agttggtgaa gagataccgc acgccggaga
     3001 gcgcggtgag gaccgtgacg atcatcatga ggacgtaggg acctatgatc aggagacggt
     3061 tcggatggcc cgattgccag acatcgcagg cgctcacgat atcgatgtgc gatttgtcga
     3121 tgaaggggga cgcgacatcg atccccgagc tgcggacgat gaacaccatg atgatgacga
     3181 tgatcgagat catctgaaag gcggttttga tctttccgaa ctggctggtg cgcaactcct
     3241 cgcccttctt gatggccaga tagcgcatga gggtgatcat gaggtcccgc ccgaagatga
     3301 cgaacaccat ccacagcgac accagcggat cgaggatgac gaaggcgacc agcgccgcga
     3361 tcacgagaaa cttgtccgcg agcgggtcga gaaatttgcc gaagctggtc tcctgattga
     3421 gcttgcgcgc gctccaaccg tcgaccagat cggtaagcga ggcgaaggcg aagacgacca
     3481 gcgcccagat gcgcgacgaa aacgacggca cgaacagcag gtacaaaaac agcgggatca
     3541 ggacaatgcg caatgcagag agcaggttcg gaatattcac aagggaactc ccatcaggtc
     3601 gtactccgtg gcgtcggtaa cccgcgccgc gacaatagca tgacgctcga ttccggggcc
     3661 ggtcaagtaa aaaattccgt ccacctcggg cgcgtcatac tgcgtgcgcc cgacccacac
     3721 gtccccctcc aggtgttcct cgacgaggac ctccacgcgc tctccgatca tggcgcgtag
     3781 ccgtcgcttc gatatcgcct gctggacctt catgagctcg tcgaatcgcc ggcgcccgac
     3841 cgccttgggg acccgccccc ttcgcgaggc cgcgtcagtc ccctcctccg gcgaatacac
     3901 gaagcaaccg gcgcgatcga tcgccacccg gcgcacaaac gtcacgagct cccgaaagtg
     3961 gcggtccgtc tccccgggaa accccaccat gaaggtcgag cggatccgga tacccggaac
     4021 gcggtcgcgg agcgtttcga tcaactctcg atagcgcgcg ccggtgccgc cgcgtcccat
     4081 cgcgcgaagg atctcgtcgc tcgcgtgctg aagcgggatg tcgaggtact tcaccactga
     4141 gttgttcgtg gccaccagat cgatgatggc gtcgtcgaca tggtccgggt gcagatacat
     4201 cagcctgatc cagcgaacgc cgttgatgcg cgccatccgc tccacgagct cgggcaggcg
     4261 cgttcgcccg taacgatagc gggcgatgtc ctgccccacg acaacaagct cgcgcgcgcc
     4321 gcgttggacg gcccgccggg cctcgtcgac gatccgtgcg ggcgtgtccg agacatgcgg
     4381 cccgcgtatc agcgggatcg cgcaatagct gcaattgttc gagcatccct ccgagatctt
     4441 gatataggca tgcggctcgc cgggaacaag tggacactgg cgatacaccg gcgccgacgc
     4501 cgcacggacg ccgatcgcac gcagcattcg cgccaccatc gcatcgtcgg gaatgcccgc
     4561 gatgaagtcg atctcgggaa tttcccgacg catatcgtcg aggtagcgct gcgcgaggca
     4621 ccccatcacc acgatcttct ggccgccgca acgcgagcgt atcgcctcga agatcgcgtc
     4681 gatcgactcc gtcttcgcgt cttcgataaa accgcacgtg ttgatgatga cgatgtccgc
     4741 atcgtcgccc gtgacgggga cgagaccggc ggacgcgagc gcaccgttga cgcgctccga
     4801 atcgacctgg tttttggaac aaccgagcga gaggattgaa aaggtgcgtg ctcgcgggga
     4861 gggacggctc atgggaacgg gttgcccgct ccatccgctt cgttccggat ggagcggaat
     4921 cagcccccta ccagtctttc acgactatct tgtattcgtt cggattcttc gagtccttcc
     4981 gccaggtgat caccttgttg gcgacctggc cggggaggcc aaacacgtgc ggcttgccgt
     5041 tgatgcgcgc ggtcacgccg ccggcattgc cgattttcac ctggatgtac tccaccgcct
     5101 cccatttctc gcgggttccc ggctggacaa ggcctttgcg cttgttgacc ccgtcgagat
     5161 acagatcgag ataggtctgc ttgttgaatt cgacctcgaa tacgatcctg aggtttttct
     5221 cgctctgcgt ggtcaccttc gtgtggtcgc ccaccacctg ccccttgtcg
//
LOCUS       DAORHF010000254         5256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16292_length_5256_cov_6.329744, whole genome shotgun sequence.
ACCESSION   DAORHF010000254 DAORHF010000000
VERSION     DAORHF010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16292_length_5256_cov_6.329744"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PKJ16_15975"
     CDS             complement(<1..546)
                     /locus_tag="PKJ16_15975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48542.1"
                     /translation="MKKYIRSSIALLLATGFALGCLACAGAPAEKKPSGASFHPNLQR
                     YSDPEINKIIILAWQKFQDGEYESAAIDFEQLERNKYVDYDITFGAGISNFRRGNLRK
                     AMQYLSQTLTLRPDHFEALMQRAAAFERLGSTGPSRRDYEAVLALTYGERLVCGFYFT
                     ENDIATASHLARRQAEARAALE"
     gene            complement(592..798)
                     /locus_tag="PKJ16_15980"
     CDS             complement(592..798)
                     /locus_tag="PKJ16_15980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HNV48543.1"
                     /translation="MRMAQDSGKGEQHIYGVVKVSPKGQVIIPADLRAELNIKPGEQL
                     VATRSRDGKGVLLLKMNALRILCS"
     gene            982..1500
                     /locus_tag="PKJ16_15985"
     CDS             982..1500
                     /locus_tag="PKJ16_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48544.1"
                     /translation="MASLIDEMTPHEAREFFSKGWITHDAMWYYHAIQECGVSVANRL
                     NMAAVKTMSMIEIQRIRKRLGAGGGPVRDFEEFKRVFSQSFDLIKPDFMHCHYSFPAE
                     NILRAGFKECFAYEGVKKAGLVEHYQCGVIERIKGWLEAMGIAYEINPQFSGCLMHQT
                     GSCSIDFLLRFT"
     gene            1621..2691
                     /locus_tag="PKJ16_15990"
     CDS             1621..2691
                     /locus_tag="PKJ16_15990"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HNV48545.1"
                     /translation="MKHDRNTAPYRREVLPPPESLVTNGRFNLGTFNGPIANVNPLDA
                     RRPLGVPLPRALAGFRLKEWEALQLGNEQCFMLVVLYDLKLASLVQFIYYDRREGNKH
                     LYETIVPSWRVRVPSSLRDTVARYRSRRFSIEIHNDLARGLVTVSVSASSFKRLPDVR
                     GRFECLHAINIEPIVVCQPFAENRAMYSHKCLMPMRGALTLGGREIAFDPASSFAILD
                     DHKGFYPYALRYDWVTGAGFDSRKRLVGFNLTDNQVRDPERYNENCLWISGRMHPLPP
                     IRITRPDGDMGRWSVRDAFGMVDIGFTPVVDGVIDMNLGAVKIDYHGPFGEYDGCIVD
                     ANGKKVSVETFFGMGERKYLRA"
     gene            complement(2697..4514)
                     /locus_tag="PKJ16_15995"
     CDS             complement(2697..4514)
                     /locus_tag="PKJ16_15995"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNV48546.1"
                     /translation="MLDALPGKTMPEIFFHNCGVYGERACVSAKDRATGAFGDISWGE
                     MDAMVRRLAYHLIAGGVRPGDRIAVFSPNRYEWWVADLAILSVGAVNVPIYPTNSIDE
                     TRYIFEHAGVSMCFAASQEHLLKALSAAKGLPGMRQIVVFDDLAALDERIMTFAAALE
                     NGAKFPHEAELAARLAQIDPETMATIIYTSGTTGNPKGVMLSHRNFVSNARQVYTKFK
                     EYIDDRQVLLSFLPLSHSLERTAGYYMPMAAGCTVAFCEDFTGIIADMQAVRPTFIIS
                     VPRLYEKIHTVIRSRLPDASIAKKLLFRLAMGTARRNLPLETNNVSRAGIFALRYAIF
                     NRLVYARVKEALGMDRMQFAVSGGAPLAVSDAEFFIGMGIRILEGFGLTETTPVASVN
                     TPWRIRPGTVGAALPETELTVSDDGELLIRGPQVMMGYYRDERATAEAFTADGFFRTG
                     DLAAIDDDGYVRITGRIKDIIVTAGGKNISPQNIENSLKTSIYIEQVAVIGDRRKYLT
                     ALVIPAFEPLRLWAERAGIAHADMAELIRNDRVIALFQAEIDKRMASLARVETIKRFT
                     LLDAEWTQDTGELTPSLKVKRRVLEEKYRNEIEAMYPPE"
     gene            complement(4556..>5256)
                     /locus_tag="PKJ16_16000"
     CDS             complement(4556..>5256)
                     /locus_tag="PKJ16_16000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015949184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HNV48547.1"
                     /translation="ISRADMENMAAWSNRKAADAKTAGKFKKEIIPVTGVDDNGNEML
                     VEHDQWIRDTVDTAKMATMSSPYKPNGVVTAATSSPLTQGACAVLLMGRKKADEMGLS
                     YTHKYRAGVLAGCDPTLMGIGPIPAVKKLFGKTGLSAKDIGPIELNEAFASQALACIR
                     DLKLDNENAPFERVNVWGGAIALGHPLGQSGARVIVTLLNIMKTDFADARYGMASLCG
                     AFGNAAAMVVERVA"
BASE COUNT          968 a         1688 c         1567 g         1033 t
ORIGIN      
        1 ttcgagcgcc gcgcgggcct ccgcctgccg ccgcgcgagg tgcgacgcgg tggcgatatc
       61 gttctcggtg aaatagaacc cgcacacgag ccgctcgccg tacgtcaggg ccagcacggc
      121 ttcgtaatcg cggcgcgacg gtccggtgct ccccagccgt tcgaaggccg cggcgcgctg
      181 catgagcgcc tcgaagtgat cggggcgcag cgtgagcgtc tgcgaaagat actgcatggc
      241 cttgcgcagg ttgccgcgac ggaaattgct gatccccgcg ccgaaggtaa tgtcgtaatc
      301 cacgtacttg ttccgctcga gctgctcgaa atcgatcgcc gcggattcat actcgccgtc
      361 ctggaacttc tgccaggcga gaatgatgat cttgttgatc tcgggatcgg aatagcgctg
      421 caggttggga tggaacgatg cgcccgacgg cttcttctcg gccggcgcgc cggcgcatgc
      481 caggcatccg agcgcgaaac cggttgcgag caatagcgcg atactggagc gaatgtactt
      541 tttcatgaaa aatctcctcc gtgcaatgag aatacggata cagccattac atcagctgca
      601 cagaatgcga agcgcgttca ttttcagcag cagtacgccc tttccatcac ggctccgtgt
      661 ggccaccagc tgctcgccgg gcttaatgtt cagttcggcg cggagatcgg ccgggatgat
      721 cacctgcccc ttcgggctca cctttaccac gccgtatatg tgctgttcac cttttccgga
      781 atcctgcgcc attctcattc actcgttcct gccgggttca gtttacacca tcatgggcat
      841 ttgttttgtc aaacattatt ataccacaaa acacgataat aatgaatagt attacttgtt
      901 ttacatttca tactattaat acttgacgac actccttctc ggattatcgt tgccgaaaac
      961 gcacgaaaac aggaggacgc catggccagt ctcattgatg aaatgacccc gcatgaagcg
     1021 cgcgaatttt ttagtaaggg atggataacg catgatgcga tgtggtacta ccacgccata
     1081 caggaatgcg gggtttccgt tgccaaccgc ctcaacatgg ctgccgtgaa aacgatgtcc
     1141 atgattgaga tacagcgcat ccgcaaacgc ctgggggcgg ggggaggccc cgttagagac
     1201 ttcgaggaat tcaagcgagt gttctcccaa tcgttcgatc tcattaaacc tgacttcatg
     1261 cactgtcact actccttccc cgcggagaac attctgcgcg ccgggttcaa agagtgtttc
     1321 gcctatgagg gagtaaaaaa ggccggcctt gtcgagcact accagtgcgg cgtcatcgaa
     1381 cgcataaagg gatggcttga ggcgatgggg attgcttacg aaatcaatcc gcaattttcg
     1441 ggatgcctca tgcaccagac tggatcatgc tctatcgatt ttttattgcg attcacctga
     1501 tcgtgaactc tcattgtatt gataacacgg cgcatccctc gaaaaaccgg ttgcacgtta
     1561 cgaccgcccc cgatagacag gcggcaggca caagaatcgc cgcatcaaac gggtatcgcc
     1621 atgaaacatg atcgcaacac agcgccttat cgccgggagg tgctcccgcc cccggagagc
     1681 ctggtcacca acggccggtt caatctgggc acgttcaacg ggcccatcgc gaacgtcaat
     1741 ccgctcgacg cccgccggcc gctcggcgtg ccgctgccgc gcgccctcgc gggattccgc
     1801 ctcaaggagt gggaggcgct gcagctggga aacgaacagt gcttcatgct ggtcgtgctc
     1861 tacgacctga agctggcctc gctcgtgcag ttcatctatt acgaccggcg ggaggggaac
     1921 aagcatctct acgagaccat cgtgccgtcc tggcgcgtgc gcgttccgtc gtcgctccgg
     1981 gacaccgttg cgcgctatcg aagcaggcgc ttctcgatcg agatccacaa cgatctcgcg
     2041 cggggcctcg tcaccgtgtc cgtatccgca tcgtcgttca agcgccttcc cgacgtgcgc
     2101 ggccggttcg aatgcctgca cgcgataaac atcgagccga tcgtggtgtg ccagcccttc
     2161 gcggagaacc gcgccatgta ttcgcacaag tgcctgatgc ccatgcgggg cgcgctcacg
     2221 ctgggcggac gcgagatcgc gttcgacccc gcctcgagct tcgccatcct cgacgaccac
     2281 aagggcttct acccctatgc gctgcgctac gactgggtca cgggggcggg gttcgattca
     2341 cggaagcggc tcgtcggctt caacctcacc gacaaccagg tgcgggaccc ggagcgttac
     2401 aacgagaact gcctgtggat cagcggccgc atgcatccgc tcccgccgat tcgcataacg
     2461 cgtcccgacg gagacatggg ccgctggtcc gtccgggacg cgttcggcat ggtcgatatc
     2521 ggattcacgc cggtcgtgga cggcgtcatc gacatgaacc tgggagcggt gaagatcgac
     2581 taccacggcc ccttcgggga gtatgacggc tgtatcgtcg atgcgaacgg aaagaaggtt
     2641 tccgtggaaa ccttcttcgg tatgggcgag cggaaatacc ttcgcgcctg agcccgtcat
     2701 tccggcgggt acatcgcctc gatctcgttc cgatacttct cctcgagcac gcgccgcttc
     2761 accttgagcg agggcgtgag ttcgcccgtg tcctgcgtcc actcggcgtc cagcagcgta
     2821 aaacgcttga tcgtctcgac ccgcgcgagc gaggccatgc gcttgtcgat ctccgcctgg
     2881 aagagcgcga tcaccctgtc gttgcggatg agctccgcca tgtccgcatg cgcgatcccg
     2941 gcgcgctcgg cccacagcct gagcggctcg aaggcgggga tgacgagcgc cgtaagatac
     3001 ttgcgccggt cgccgatcac cgccacctgt tcgatgtaga tcgacgtctt caggctgttc
     3061 tcgatgttct gcggcgagat gttcttcccg cccgccgtca cgatgatgtc cttgatccgc
     3121 ccggtgatgc gcacgtagcc gtcgtcgtcg atcgccgcca gatcgccggt ccggaaaaaa
     3181 ccgtcggcgg tgaaggcctc ggccgtcgcg cgctcgtcgc ggtagtagcc catcatcacc
     3241 tgcggccccc gaatgagcag ctcgccgtcg tccgagaccg tgagctccgt ctccgggagc
     3301 gcggcgccca ccgtgccggg cctgatccgc cagggcgtgt tcacgctcgc caccggcgtc
     3361 gtctcggtga ggccgaaacc ctcgaggatc ctgatcccca tcccgatgaa gaactcggcg
     3421 tcggagaccg cgagcggcgc gccgccggag accgcgaact gcatgcggtc catcccgagc
     3481 gcctccttga cccgcgcata gaccagccgg ttgaagatcg catatcgcag agcgaagatc
     3541 cctgcgcggg agacattgtt ggtttccagc ggcaggttcc tgcgcgcggt ccccatcgcc
     3601 agccggaaga gcagcttctt tgcgatcgac gcatcgggca gccgcgagcg gatgaccgta
     3661 tggatcttct cgtacagccg cggcacgctg atgatgaagg tgggccgcac cgcctgcatg
     3721 tcggcgatga tgcccgtgaa gtcctcgcag aaggcgacgg tgcacccggc ggccatgggc
     3781 atgtaatacc cggccgtccg ctcgagggaa tgcgacagcg gcaggaacga gaggagcacc
     3841 tgccgatcgt cgatgtattc cttgaacttc gtgtagacct gccgcgcgtt cgaaacgaaa
     3901 ttccggtgcg agagcatcac ccccttcggg ttgccggtcg tgccggaggt gtagatgatc
     3961 gtggccatcg tctcggggtc gatctgcgcg aggcgcgcgg cgagctccgc ctcatgcggg
     4021 aatttcgccc cgttttccag cgccgcggcg aaggtcatga tccgctcatc gagcgccgcc
     4081 aggtcgtcga agacgacgat ctgccgcatt cccggcagtc ccttcgcggc cgagagcgcc
     4141 ttgagcaggt gttcctgcga cgcggcgaaa cacatcgaca cgcccgcatg ctcgaagatg
     4201 tagcgcgtct cgtcgatgga gttggtggga tagatcggca cgttcaccgc gcccaccgaa
     4261 aggatcgcga gatccgccac ccaccactcg taccggtttg gcgaaaacac tgcgatgcgg
     4321 tctcccggcc gcaccccgcc ggcgatcaga tgatacgcca gccgtcgcac catcgcatcc
     4381 atctcgcccc acgagatgtc gccaaacgcg ccggtcgccc ggtccttcgc ggaaacgcag
     4441 gcgcgctcgc catacacccc gcagttgtga aaaaaaatct ccggcatggt ctttcccggc
     4501 agcgcgtcaa gcatctcgtc cacctccagt ttgattcgcg ccgcggaacg ggccgtcagg
     4561 ccacccgctc cacgaccatc gccgccgcgt tcccgaaagc cccgcagagc gaggccatcc
     4621 cgtagcgagc gtcggcgaaa tcagtcttca tgatgttgag cagcgtcacg atcacccgcg
     4681 cgcccgattg gccgagcggg tgcccgagcg cgatcgcccc gccccatacg ttcacgcgct
     4741 cgaagggcgc gttctcgtta tcgagcttga gatcgcgaat gcaggcgagc gcctggctcg
     4801 cgaaggcctc gttgagctcg atggggccga tgtccttcgc ggacaggccg gtctttccaa
     4861 agagcttctt caccgccggt atggggccaa tgcccatgag cgtgggatcg catcccgcca
     4921 gcacgccggc tcgatacttg tgcgtatagg agagccccat ctcgtccgct ttctttcgac
     4981 ccatgagcag caccgcgcag gcgccctgcg tcagggggga tgatgtcgcc gccgtcacca
     5041 caccgttggg cttgtacggc gagctcatcg tcgccatctt tgccgtgtcc acggtgtcgc
     5101 ggatccattg gtcgtgctcc acgagcatct cgtttccgtt gtcatcgacg cccgtgacgg
     5161 ggatgatttc cttcttgaac ttgccggccg tcttggcgtc ggcggccttg cggttggacc
     5221 aggccgccat gttctccatg tcggcgcgcg agatgt
//
LOCUS       DAORHF010000255         5228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16439_length_5228_cov_4.653779, whole genome shotgun sequence.
ACCESSION   DAORHF010000255 DAORHF010000000
VERSION     DAORHF010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16439_length_5228_cov_4.653779"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PKJ16_16005"
     CDS             complement(<1..566)
                     /locus_tag="PKJ16_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HNV48548.1"
                     /translation="MAHIDQPSTIRSGEELDAAKVRDFLLGAIPGLSGDIAIKQFPSG
                     FSNLTYLVSVGDTEMILRRPPFGKKAKTAHDMGREYRMLTALKPHFPYCPTALVYSED
                     EGVMGCPFYVMERLKGIILRKDLPAGMALAPAEARRLSENLIDVLAELHTIDYAAAGL
                     AEFGKPEGYVRRQIDGWSARYRDARTTDA"
     gene            complement(581..1315)
                     /locus_tag="PKJ16_16010"
     CDS             complement(581..1315)
                     /locus_tag="PKJ16_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48549.1"
                     /translation="MAATAEQRVGFGAFRAEVSLTVEDICRDYFRENRATLKIKKEAV
                     AVPNLAAFVNATLKLSSAVGFDAMSLRDLCSESGLSMGALYAYFSSKDELLTMIQRQG
                     QATVKRILAERIDHIRDPLPRLRVAIRSHLYLSELLHQWFYFFFMETKNLKKQNRTIP
                     IESELYTERIITDILTAGRDEGVFSVENIELTGAVIKAMLQDWYLKRWKYSQRKVTVD
                     EYASFVEGFIESAIVRERAPNGGERP"
     gene            complement(1317..2513)
                     /locus_tag="PKJ16_16015"
     CDS             complement(1317..2513)
                     /locus_tag="PKJ16_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HNV48550.1"
                     /translation="MDFGISEKMQAVLGMINEFVDRELIPLEREFLVNEFRDMLPVLE
                     QKRAMVKKMELWGPNHPTELGGMGLSMLEHGLVSEALGRSPLGHYVFGCQAPDAGNIE
                     ILHQHGTEEQKDAYLAPLAAGKIRSCFAMTEVDMPGSNPVMMDTTAVKDGDDYVINGH
                     KWYTTGADGATFAIAMAITDPEAPVYLRASMIIVPTDTPGYNLVRNIPVMGHSGSDYA
                     SHGEVLFQSCRVPRKNLLGKEGMGFVIAQDRLGPGRIHHCMRWLGICSRAFDLMCRRA
                     NERPIAPNGKTLATRQIVQEWVADSAAEIQAARLMTLYAAWRMDTAGHKEARDDVSMI
                     KYVVANTMQRVVDRALQVHGGLGMTDDTVLAYFFRHERAARIYDGADEVHKTSFAKRC
                     LARYAG"
     gene            complement(2540..3250)
                     /locus_tag="PKJ16_16020"
     CDS             complement(2540..3250)
                     /locus_tag="PKJ16_16020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HNV48551.1"
                     /translation="MRIISTIYFIRHGQASFGSGNYDKLSDTGMRQARLLAEHFNALG
                     IRFDAAYSGTLVRHAETAQSFMGAYPGPAPGITTLDGLNEYQSREILTALVPILLEEQ
                     PSMKEHSGRLLQDKRSFQTVFEAVMLMWASGRHDVPGLQRWRDFQDAVRSAVTQIMRT
                     DGSGKRVAVFTSGGPISVIAQQALGLDDGATMRLREQVVNSSVSRFKCTPERIMMSTF
                     NEYTHLERAGDASLITYR"
     gene            3443..4222
                     /locus_tag="PKJ16_16025"
     CDS             3443..4222
                     /locus_tag="PKJ16_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014577511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV48552.1"
                     /translation="MSPKEKTILSEEQTFTYFEKAPTIDPGEFQKVIDSRRSVRVFDD
                     TKIPEDIVRTCIENGLLAPNSSNLQPWEIYWVSSPEKKRELARYCLSQIAANKAAELI
                     VLVARTATWRQHAREMVTLFTSGPDKAPAMAIAYYKKIVPFVYTQGFLGILGFLKRIA
                     FFVKGLSSPVIREPVTRAHLRTWAIKTTALAAENIMLSFRAYGYDTCPMEGYDSKRIK
                     KLLGLPRDSAVVMVIAAGKRTEGGIYGQRIRYDSARFIKKV"
     gene            4230..5006
                     /locus_tag="PKJ16_16030"
     CDS             4230..5006
                     /locus_tag="PKJ16_16030"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNV48553.1"
                     /translation="MRILVCIKQVLDSESPIVLDERAHWIEEHTRCTWIMNRYDEFAL
                     EEALLIGERHADVHIDALTVGPPRAAAVVKKALEKGAHDAIHLRVDDPSLSAAQTAAL
                     IAGYIADTSYDLILTGVMSEDLMQAQVGPTLAALRGVPCAASVVSQSCDIDTGTIRAR
                     IELDGGSHEVVDLPMPCVLTIQSGINQPRYPSLSNVLRAKKVEPRTAICDMATVAARR
                     DRVESIDFPERLKNVIMIDGTPGEKAAVLLSLLNEKGVLR"
     gene            5009..>5228
                     /locus_tag="PKJ16_16035"
     CDS             5009..>5228
                     /locus_tag="PKJ16_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48554.1"
                     /translation="MDRVYLVLDTVNNSIPRQSFDVLKCAAVMAGNDRSRILAIVPGS
                     DCTKHAQSCSDEFGIATIALEHHGLRLPN"
BASE COUNT         1036 a         1614 c         1488 g         1090 t
ORIGIN      
        1 gcatcggtcg tgcgcgcatc ccggtatcgc gcgctccacc catcgatctg gcggcggaca
       61 tacccttccg gctttccgaa ttccgcgagg cccgcggccg cgtaatcgat cgtatggagc
      121 tcggcgagca cgtcgatgag gttctccgag agtctgcgcg cctctgcggg ggcgagcgcc
      181 atcccggcgg gaaggtcctt tctcagtatg atccccttta ggcgctccat gacataaaac
      241 gggcacccca tcacgccctc gtcctcggag tagaccagcg ccgttgggca atagggaaaa
      301 tgcggcttga gcgcggtgag cattcggtat tcccgcccca tgtcgtgcgc ggtctttgct
      361 tttttgccga acggcggccg tcgcagtatc atttccgtat cgccaacgga aacaagatag
      421 gtgagattcg aaaacccgct tgggaattgc ttgatggcaa tgtcacccga gagcccgggt
      481 atggctccca agagaaaatc tcggaccttt gccgcatcga gctcttcacc ggaacgaatg
      541 gttgatggtt ggtcaatgtg tgccatgtcg tttctccttg ctatggcctt tcgccgccgt
      601 tgggcgcgcg ttcacggaca atcgccgatt cgatgaatcc ttcgacgaac gaggcgtact
      661 cgtccaccgt caccttccgc tgggaatact tccagcgctt gagataccaa tcctgcagca
      721 tcgctttgat caccgcgccg gtgagctcga tgttctccac cgagaacacg ccctcgtcac
      781 gccccgccgt caggatgtcg gtgatgatcc gctcggtgta caattcgctc tcgataggga
      841 tcgtgcggtt ttgctttttc aggtttttcg tttccatgaa gaaaaaataa aaccattggt
      901 gcaacagttc gctcaggtac agatggctcc gtatcgcgac gcgcaggcgg ggcaggggat
      961 cgcgaatatg gtcgatccgc tccgcgagga tgcgcttcac ggtcgcctgt ccctgacgct
     1021 gtatcatcgt gagcagctcg tccttgctcg aaaagtacgc gtacagcgcc cccatgctca
     1081 gtcccgattc gctgcagagg tccctgaggc tcattgcgtc gaagcccacc gcgctgctca
     1141 gcttgagcgt cgcgttcacg aacgccgcga gattggggac cgcgaccgcc tcctttttaa
     1201 ttttaagagt cgcgcggttc tcccggaagt agtcgcggca gatgtcctcc accgtgagcg
     1261 atacctccgc gcggaaggcg ccaaacccga ccctctgttc cgcagtggcg gccatattac
     1321 ccggcatacc gcgcaaggca gcgtttcgcg aacgaggtct tgtggacctc gtcggcgccg
     1381 tcgtagatcc gcgcggcgcg ctcgtgtcgg aagaagtagg cgagcacggt gtcgtcggtc
     1441 atccccagac ctccatggac ctggagcgcg cggtcaacca cccgctgcat ggtgttcgcc
     1501 acgacgtatt tgatcatgct cacatcgtcg cgcgcctcct tgtggccggc ggtgtccatc
     1561 cgccacgccg cgtagagggt catcaagcgc gccgcctgta tctccgcggc ggaatcagcc
     1621 acccactcct ggacgatctg ccgcgtcgcg agcgtctttc cgttgggcgc gatcggccgc
     1681 tcgttcgcgc gccggcacat cagatcgaag gcgcggctgc agatgccgag ccagcgcatg
     1741 cagtgatgga ttcgccccgg ccccagccgg tcctgcgcaa tcacgaatcc catcccctcc
     1801 ttcccgagaa ggttcttccg cggcacccgg cacgactgga acagcacctc tccgtgactg
     1861 gcatagtcgc tgccggagtg gcccatcacg ggaatgttgc gcaccaaatt atacccgggc
     1921 gtatcggtcg ggacgatgat catgctcgcc cgaaggtaca ccggcgcctc gggatcggtg
     1981 atcgccatcg cgatggcgaa ggtcgcgccg tccgcgccgg tcgtgtacca cttgtggccg
     2041 ttgatcacgt aatcgtcgcc gtccttcacg gccgtcgtgt ccatcatcac cgggttcgat
     2101 ccgggcatgt ccacctcggt catggcgaaa caactgcgaa tcttcccggc tgcgagcggg
     2161 gcaagatatg catccttctg ttcctcggtc ccatgctgat gcagaatctc gatgttgccc
     2221 gcatccggcg cctggcaacc gaacacatag tgcccgagcg gcgagcgccc cagcgcctcg
     2281 gagaccagcc cgtgctcaag catggaaagc cccatgccgc cgagctcggt ggggtgattg
     2341 ggaccccaga gctccatttt tttcaccatc gcccgttttt gctcgaggac cgggagcatg
     2401 tcgcgaaact cgttcaccag gaactcgcgc tcaagcggga tgagctcccg gtccacgaac
     2461 tcgtttatca ttccgagaac cgcctgcatc ttttccgata tgccaaaatc cattgtcgtc
     2521 ctccctgcgc cttcgggcgc tatcgatagg tgatgagcga cgcgtccccc gcgcgctcga
     2581 gatgcgtgta ttcgttaaac gtcgacatca tgatccgttc cggtgtgcac ttgaatctgc
     2641 tcaccgatga gttcacgacc tgttcgcgga ggcgcatcgt tgcgccgtcg tccaggccga
     2701 gcgcctgctg cgcaatgacc gaaatcggcc cccccgacgt gaacacggcc acgcgtttcc
     2761 ccgatccgtc cgtgcgcatg atctgcgtca ccgcagatcg gacagcgtcc tggaagtcgc
     2821 gccagcgttg caatccggga acatcgtgcc gcccggaggc ccacatcagc atgaccgcct
     2881 cgaaaacggt ttgaaacgac cgtttgtcct gaagcaaccg tcctgagtgt tccttcatcg
     2941 agggctgctc ctcgaggagt atcggaacaa gcgccgtaag gatctcccgc gactggtact
     3001 cgtttaatcc atccagcgtg gtgatcccgg gcgcgggccc ggggtaggcg cccatgaacg
     3061 attgcgccgt ttcggcatgt cgaacaaggg tgccggagta cgcggcatcg aagcggatcc
     3121 ccagcgcatt gaagtgctcc gccagaagcc gcgcctggcg catccccgta tcggagagct
     3181 tatcgtagtt cccgctcccg aacgaggcct gtccatgccg tatgaaataa atcgtgctaa
     3241 taattctcaa ctccacgacc atcatgcatg gtggtttata aatgtcaata aatatttcga
     3301 gcgctcgcta tattttttgt tgacaaatca accttccatt tttttattac gattagagcg
     3361 tataacagta ggttcattgc caattgaacc gtacgagatg gctggaatag gttctttgag
     3421 attatgaaaa catgaggtag acatgtcccc aaaagagaag accatccttt ctgaagaaca
     3481 gacctttacg tatttcgaga aggcgccaac gattgatccc ggggaattcc aaaaggtcat
     3541 cgattcgcgc cgctcggtcc gtgtttttga tgatacgaag atccctgagg atatcgttcg
     3601 aacatgcatc gaaaatggcc tcctggcgcc aaattcttcc aatctgcagc cgtgggagat
     3661 ctattgggtt tccagtcccg agaaaaaacg ggagctcgcc cgctattgcc tatcgcagat
     3721 agcggcaaac aaggccgccg agctcatcgt cctcgtcgcg cgcacggcga cctggcgaca
     3781 gcatgcccgg gagatggtga ccctcttcac gtccggaccg gacaaggccc cggcaatggc
     3841 gattgcgtac tacaagaaga tcgttccgtt cgtatatacg cagggattcc tcggcatcct
     3901 gggcttccta aagcgaatag cgttctttgt aaagggactg tcgtcacccg tcatccgcga
     3961 acccgtcacg cgcgcacatc ttcgcacctg ggcgatcaag acgacggcgc tcgccgccga
     4021 aaatatcatg ctctcgtttc gcgcatacgg ctacgacacc tgtcccatgg aggggtacga
     4081 ttcgaagcgc atcaaaaaat tgctcggact gccgcgcgat tcggcggtcg tcatggtgat
     4141 cgcagccggg aagcgcaccg agggcgggat ctacggacag cgaattcggt atgattctgc
     4201 gcgtttcatc aaaaaggtct aaccggtata tgcgcattct cgtctgcatc aagcaggtgc
     4261 tcgattccga atcgccgatc gtactcgacg agcgggccca ttggatcgag gaacacacgc
     4321 gctgcacgtg gatcatgaac cgctacgacg agttcgcgct ggaagaggcg cttctgatcg
     4381 gagaacgaca cgccgacgtc catatcgacg cgttgaccgt gggaccgccg cgcgcggcgg
     4441 cagtggtcaa aaaggcgctg gagaagggcg ctcatgacgc gattcacctg cgtgtcgatg
     4501 atccctcgct ttcagctgcg cagacggccg cgctcatcgc gggttacatc gcggatacat
     4561 catacgatct catactgaca ggcgtcatgt ccgaggacct catgcaggcg caggtcgggc
     4621 cgaccctggc cgcgctcaga ggcgtcccgt gcgccgcatc tgtcgtgtcg cagtcatgcg
     4681 atatcgacac ggggacgatt cgggcgcgca tcgagctgga cggcggcagt cacgaggtgg
     4741 tcgatctccc catgccctgc gtcctgacga tccagtcggg catcaaccag ccgcgatatc
     4801 cgtctctgtc caacgttctc cgggcaaaga aggtcgaacc gcgaacagct atatgcgata
     4861 tggcgaccgt ggcggcacgg agagaccggg tggaatcgat cgattttccc gagcgcctca
     4921 agaatgtcat catgatcgac ggaacgcccg gcgagaaagc cgcagtattg ctttcgttac
     4981 tgaacgagaa gggcgttttg agataatcat ggaccgggta tatctcgttc tcgacaccgt
     5041 aaacaattcg ataccgcgac agagcttcga cgtgctcaaa tgcgccgcgg taatggcggg
     5101 aaatgaccgg tcacgaattc tcgcgattgt gcccggaagc gattgtacga aacatgcgca
     5161 gtcatgttcc gatgagttcg gcatcgccac catcgccctc gagcatcacg gccttcgcct
     5221 ccccaacc
//
LOCUS       DAORHF010000256         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16479_length_5220_cov_5.834850, whole genome shotgun sequence.
ACCESSION   DAORHF010000256 DAORHF010000000
VERSION     DAORHF010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16479_length_5220_cov_5.834850"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PKJ16_16040"
     CDS             complement(<1..543)
                     /locus_tag="PKJ16_16040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV48555.1"
                     /translation="MRPFESEADLAARGVIGAHDLPPEGSAPFVPKRITLQWHITGRC
                     NLSCRHCYDTREHQDELPLADLRTILDAFTARVAAWNVPAHINITGGEPLCREDLFDL
                     LASIGPSTERFGFGILTNGTRIGPDEARRLKRLGCRFVQVSLEGGEAANDAIRGANSY
                     RDIRRAVRALRRQRIYTMVSF"
     gene            complement(550..1419)
                     /locus_tag="PKJ16_16045"
     CDS             complement(550..1419)
                     /locus_tag="PKJ16_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48556.1"
                     /translation="MMTPRIVALLLFACLCAGSCGCGGSHDYAVEEYRAAFAVPATGD
                     HADVTLDILYDIGSTPKAEGFKFVGTGPVTNLVAHDDRGAVVRCETRQMRETRISWHF
                     DPVANARKRIIVSFRLPGALQRDGSTLTLDAPWAGVFRVPVRHARYEIIFPPRTSPRI
                     LSSTPGGGIESLQGALVFIARQSPLSEKRFAVSFDSGGPHVTRPEIQPKRAIPATPRT
                     ERSSDTYASILREHIPVVVVSMVIFFILFNFFRVMTRKGGTGSSGGCSSSSCSGGGCG
                     GGCGGGCGGGCGG"
     gene            complement(1416..2171)
                     /locus_tag="PKJ16_16050"
     CDS             complement(1416..2171)
                     /locus_tag="PKJ16_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48557.1"
                     /translation="MNAINAVIDTLNVPPAVKTDLSRFIQELITLYREDLHSITAFGS
                     SVRGEYREKTSDLNLLVVYHDLEIADLARVAEVARRWQRKRNFAPRFLSKRNLVDTAR
                     YFQVDVLHLKHTSALLFGHDILGGLPESLPDMRWQIAHETKKIRMRLKQQFWRTAGDR
                     AAMAAVIARRSSSLVSLLRATLYLAERQYHETPWQVIDAASRNLGIDAGALERLLALK
                     HAARPNAAELNEAFTALMNIIRALDAKIETIPA"
     gene            2325..3884
                     /gene="murJ"
                     /locus_tag="PKJ16_16055"
     CDS             2325..3884
                     /gene="murJ"
                     /locus_tag="PKJ16_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HNV48558.1"
                     /translation="MTESSIVRSTTITALSTLASRIMGFARDMIVAAAFGASGALDGF
                     YVAFRIPNLFRRLVAEGSLTISFIPVYTDYLVNRGEREALELAQKTLSILVLVLVAIT
                     GVGIAFSPQIVSLFARGFADARIIALTVDLNRIMFPYLFLVGLVAFSMGVLNSHGRFF
                     AAAFSPVLLNVGFIIGATLLSLLFVEPLYGLALGVLIGGVLQVLLQVPDLVRSGFRLR
                     FSIDLNHPGIRRIFRMIVPALFGIAVYQLNILMSTMLASMLAEGSISYIYYSDRLNEM
                     VLGVFVMSIGNVVLPAMSRHTAREDFGRLRALYADSVSASLFVGIPACVALIAASFPI
                     VSVLFARGAFTMYHAEMTARALFFASMGIPALSVLRITTPAFYSLKDTRTPVIASTVS
                     FVVNIAAGYALMHTPLAHAGLTLGNAIASTLQTAILLAVLNRRIGGIAWGALAASVLK
                     YAVASAVMCGVIVAIAQMADWRQASFVHRAGTLAALVAAGGAAYFSVCYLLRVKELHF
                     LVSKIRSRLGR"
     gene            3990..>5220
                     /locus_tag="PKJ16_16060"
     CDS             3990..>5220
                     /locus_tag="PKJ16_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HNV48559.1"
                     /translation="MTRIRAISLAVAVIVALAAGCAREDSKPVDLSDEERQWLQQHRG
                     VIRLSPCPDFPPIDFQDRDGAHRGLTADYLAWIEKKLNYRFIIEHQRDWFTVMQKAKA
                     GEIDGVLTIQEVPERREYLLFTRPYVRIPNIIVTRSTHSGDLTPEALAGMKVSSVRGY
                     AVTDYLTTKYPALVFDHVPNELTGLMKVSFSQSDAMIVSLSTASYFIEEKGITNLRVA
                     GAVGYDWDLRIAVRKDLPILQGILDKTLASMPQDMRTEIYRRWIRLGSHSVFDNPYLR
                     IALGAAVLVALLVFLGIIAWNRMLRRQVMDRTAELSRELAERTRAEQALSQEKERLLI
                     TLRSIGDGFISTDTNGRVVLMNRIAEELTGVEVRDALGADVAQVCRIVDRESGQAQEL
                     PVARCIARLIDPSSGTIH"
BASE COUNT          822 a         1688 c         1746 g          964 t
ORIGIN      
        1 gaaggagacc atggtgtaga tgcgctgccg tcgcagggcc cgcaccgcgc gtcggatgtc
       61 gcgatacgaa ttggcgccgc gaatggcgtc gtttgccgcc tcgccgccct cgagcgacac
      121 ctggacgaag cggcagccga gccgcttgag ccgccgggcc tcgtccgggc cgatgcgcgt
      181 gccgttggtg aggatgccga agccgaagcg ctcggtggac gggccgatgg acgcgagcag
      241 atcgaagagg tcctcccggc agagcggctc gccgcccgtg atgttgatat gcgcggggac
      301 gttccatgcc gcgacacgcg cggtgaacgc atcgaggatc gtgcggaggt ccgctaacgg
      361 gagctcgtcc tggtgctcgc gtgtgtcgta gcaatgacgg caggagagat tgcaccggcc
      421 ggtgatgtgc cattgcaggg tgatccgttt cgggacaaag ggggccgatc cctccggcgg
      481 caggtcgtgt gcgccgatga cgccgcgcgc ggcgagatcg gcctcgctct cgaaggggcg
      541 catgcgctat cagcctccgc agccgcctcc gcatccgccg ccgcaccctc cgccgcatcc
      601 gccccccgag caagagctgg aggagcatcc cccggacgag cctgtgccgc cctttcgtgt
      661 cataacccga aagaagttga ataggatgaa aaagatcacc atcgatacga caacgacggg
      721 aatgtgctcg cgaagaatgc tggcgtaggt gtcgctcgat cgctcggtcc gcggtgtcgc
      781 gggaattgcg cgtttcggct gtatctcggg gcgcgtgacg tgcggaccgc ccgagtcgaa
      841 tgaaaccgcg aagcgcttct ccgagagcgg ggactgtcgt gcgatgaata cgagcgcacc
      901 ctggagggac tcgatcccgc cgcccggcgt cgaggacaga attcgcggac tcgtgcgcgg
      961 cggaaagatg atctcgtagc gcgcgtggcg caccgggacg cggaacacgc cggcccaggg
     1021 cgcgtcgagc gtgagggtcg atccgtcgcg ttgaagcgcg ccgggaagcc ggaacgagac
     1081 gatgatccgc ttgcgcgcgt tcgcgaccgg atcgaagtgc cacgatatcc gcgtttcccg
     1141 catctgcctg gtctcgcagc ggacgaccgc gccgcgatcg tcgtgcgcga cgaggttcgt
     1201 gaccggcccg gtgcccacga acttgaagcc ctcggccttg ggggtggagc cgatgtcgta
     1261 gaggatgtcc agcgtcacgt ccgcatggtc gcccgttgcg ggaacggcga aggcggcgcg
     1321 gtattcctcg acggcgtaat cgtgcgagcc gccgcacccg cacgatcccg cgcacaggca
     1381 ggcgaagaga agaagcgcga cgatccgtgg cgtcatcatg ccggtatcgt ttcgattttc
     1441 gcgtcgaggg cgcgaatgat gttcatgagc gcggtgaagg cctcgttgag ctcggcggca
     1501 ttcggccgtg cggcgtgctt gagcgcgagg agccgttcga gggcgccggc gtcgatcccc
     1561 aggttgcgcg aggcggcgtc gatcacctgc cacggcgttt catggtactg ccgctcggca
     1621 agatagagcg ttgcgcgaag caggctcacc agcgacgagc tccgccgcgc gatgaccgcc
     1681 gccatcgcgg cgcgatcacc cgccgtacgc cagaactgtt gcttgaggcg catgcggatc
     1741 tttttcgttt cgtgggcgat ttgccagcgc atgtcgggga ggctttccgg caggccgccc
     1801 aggatgtcgt gcccgaagag gagggcgctc gtatgcttca ggtggagcac gtccacctgg
     1861 aaatagcgcg cggtgtccac caggttgcgc ttcgagagga agcggggcgc gaagttgcgc
     1921 tttcgctgcc agcggcgcgc gacctccgcg acgcgcgcga ggtccgcgat ctccaggtcg
     1981 tgatagacca ccagcaggtt gaggtccgac gtcttctcgc gatactcgcc gcgcacgctg
     2041 ctcccgaacg cggtgatcga gtgcaggtcc tcccggtaga gggtgatgag ctcctggatg
     2101 aagcgcgaga ggtcggtctt cacggccggc ggtacgttca gcgtgtcgat gacggcattg
     2161 atcgcgttca tggcggcccc cacggtctgt tttcctgaag tatacgcagg gcggacggcg
     2221 atgtcaacga gaatttccgc gcgaggcgga tgcgataaaa tgatttacgg gccatgcggc
     2281 gtcgctagga tgggcgcctg cgagcgcgca ccgggggagc ggaaatgact gaatcaagca
     2341 tcgttcgatc gacgaccatc acggcgctgt cgacgctggc cagccgcatc atggggtttg
     2401 cccgcgacat gatcgtggcc gccgccttcg gcgcctccgg cgccctcgac ggcttctacg
     2461 tggccttccg catccccaac ctgttccgcc ggctggtcgc cgaggggtcg ctcaccattt
     2521 cgttcatccc ggtgtacacc gactacctgg tcaaccgcgg cgagcgcgag gcgctggagc
     2581 tcgcgcagaa gacgctttcg atcctcgtgc tcgtgcttgt ggccatcacc ggcgtcggga
     2641 tcgccttctc gccgcagatc gtctcgctct tcgcgcgcgg gttcgccgac gcgcgcatca
     2701 tcgccctcac cgtggacctc aaccggatca tgttcccgta cctgttcctg gtggggctgg
     2761 tcgccttttc gatgggcgtg ctcaactcgc acgggcgctt ctttgccgcg gccttctcgc
     2821 cggtgctctt gaacgtcggg ttcatcatcg gcgcgacgct cctgtcgctc ctcttcgtcg
     2881 agccgctcta cggcctcgcg ctcggcgtgc tcatcggggg ggtgctgcag gtcctcctgc
     2941 aggtgccgga ccttgtccgg tccggatttc gcctgcggtt ttccatcgac ctcaaccacc
     3001 cggggatccg ccgcatcttc cggatgatcg tcccggcgct tttcggcatc gcggtctacc
     3061 agctcaacat cctgatgagc acgatgctcg cctccatgct cgcggagggg agcatatcgt
     3121 acatctatta ctccgaccgc ctcaacgaga tggtcctggg cgtgttcgtg atgtcaatcg
     3181 gcaacgtggt gctgccggcc atgtcgcggc acacggcgcg cgaggacttc ggccggcttc
     3241 gcgctctcta cgccgactcc gtgagcgcgt cgctgttcgt ggggataccg gcctgcgtcg
     3301 cgctcatcgc ggcgagcttc ccgatcgtgt cggtgctctt cgcccgcggc gccttcacga
     3361 tgtatcacgc ggagatgacc gcgcgggcgc tcttcttcgc gagcatggga atccccgcgc
     3421 tctcggtgct gcggatcacc acgcccgcct tctattcgct caaggacacc cgcacgcccg
     3481 ttatcgcgtc gaccgtttcg ttcgtggtga acatcgccgc gggctacgcg ctcatgcaca
     3541 ccccgctcgc gcacgccggc ctcacgctgg gaaacgccat cgcctccacg ttgcagacgg
     3601 cgatcctgct ggcggtcctc aaccggcgta tcggcgggat tgcgtggggt gcgctcgccg
     3661 cctccgtcct caaatacgcc gtcgcgtcgg cggtcatgtg cggcgtgatt gtcgcgatcg
     3721 cccagatggc ggactggcgg caggcgtcgt ttgtgcatcg ggccgggacg cttgcggcgc
     3781 tcgtggccgc gggcggggcc gcctacttct cggtctgcta tctgcttcgc gtgaaggaac
     3841 tgcacttttt agtcagtaag atccgctcgc ggctcgggcg gtagtcggcg cgcaggcgca
     3901 cattccccga aaaaaacttg ccccgtttcc ggggatgtgc gatactatgg gtgcgccggc
     3961 cttccggtga ttgcggggtt tctggaacca tgacacgaat tcgtgcgata tcgcttgccg
     4021 tggccgtgat tgtcgcgctg gcggccggct gcgcgcgcga ggactcgaag cccgtcgacc
     4081 tctccgacga ggagcggcag tggttgcaac agcatcgcgg cgtgatacgg ctctccccct
     4141 gtcccgattt tcctccgata gatttccagg accgcgacgg cgcgcatcgc gggctcaccg
     4201 cggactatct cgcctggatc gagaaaaagc tcaactaccg gtttatcatc gagcaccagc
     4261 gcgactggtt cacggtgatg cagaaggcga aggccggcga gatcgacggc gtgctaacca
     4321 tccaggaggt ccccgagcgg cgcgagtacc tgctcttcac ccggccgtat gtgcgcatcc
     4381 ccaacatcat cgtgacgcgg agcacgcatt ccggcgacct tacgcccgag gcgctggccg
     4441 gcatgaaggt gtcctcggtg cgggggtacg ccgtgaccga ttacctgacg acgaaatatc
     4501 ccgccctcgt cttcgatcat gttcctaacg agctgacggg gctgatgaaa gtgtcctttt
     4561 cacaatcgga cgccatgatc gtaagcctct cgacggcatc ctactttatc gaagagaagg
     4621 ggatcaccaa tctccgtgtg gcgggcgccg tcggctacga ctgggacctc cggatcgccg
     4681 tgcggaagga cctccccatc ctgcagggca ttctggacaa aacgcttgcg tccatgccgc
     4741 aggacatgcg gacggagatc tatcgacgct ggatacgcct tgggtcgcac agcgtgttcg
     4801 acaatccata cctccggatc gcgctgggcg cggcggtcct ggtagcgctg ctcgtgttcc
     4861 tcggcatcat cgcctggaac cgcatgctgc ggcggcaggt catggaccgg accgccgaac
     4921 tgtcgcgcga gcttgcggag cggaccaggg ccgagcaggc tctgtcgcag gagaaggagc
     4981 gactcctcat cacgctgcgt tcgatcgggg acgggttcat cagcaccgac acgaacggtc
     5041 gcgtggtcct gatgaaccgg atcgccgagg agctgaccgg cgtcgaggtg cgcgacgcgc
     5101 tgggcgccga tgtcgcgcag gtatgccgga tcgtcgaccg cgagagcggc caggcgcagg
     5161 agcttcccgt cgcgcgctgc atcgcccgac tcatcgatcc cagcagcggc acgatccatc
//
LOCUS       DAORHF010000257         5209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16532_length_5209_cov_5.117191, whole genome shotgun sequence.
ACCESSION   DAORHF010000257 DAORHF010000000
VERSION     DAORHF010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16532_length_5209_cov_5.117191"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PKJ16_16065"
     CDS             complement(<1..1006)
                     /locus_tag="PKJ16_16065"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48560.1"
                     /translation="MANIINRDTIGDMARRAATKYGDKTAIIFRDTTLSFYDLERESR
                     RFAHLMASYGVKKGDRVAILAYNSHYYPISLIGLGKIGAVQVPINYMLNAEEIAYIVN
                     HSGAKVFIVEDALYPVIAQAQTQGKFTTVGKWGFLPLADTVVPEGFFNLMAELESMPY
                     DEPQVDVEAEDIVQIPYTSGTESKPKGAMLSHRALMSQYYSCIFDGEYQTEDVSLHAL
                     PLFHCAQLHCFLLPFIYVGATNVILHRADPVEMIKSIEKFQITHMFAPPTVWIGIINH
                     PEFKKYNHASLKKAAYGASIMPVEIIKQLSITFHGLRLWNYYGQTEMGPVATILKPED
                     Q"
     gene            1523..2431
                     /locus_tag="PKJ16_16070"
     CDS             1523..2431
                     /locus_tag="PKJ16_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV48561.1"
                     /translation="MNSEPAYLKSHTTGRLKDITDELRGVIRACALCPHRCGVDRTIG
                     ERGKCRSGALPIVSSFHAHFGEERPLVGRGGSGAIFFTNCNLACIFCQNYDISHYGRG
                     DEVSFDELAAMMLALQAKGCHNINFVSPSHMLYSIVEALLIAVPRGLSLPLVYNTGGY
                     DSANIIHLIDGVFDIYMPDFKFMDASVANELSDAPDYPERAMEAVAEMHRQVGDLELD
                     DRGIARRGLLVRHLVLPNNIARTDAVLDFLAGLSRDTYVNIMDQYRPEYRARECFDLR
                     RRVTLQEFDAAVAWARKAGLRRIDGV"
     gene            2582..3100
                     /locus_tag="PKJ16_16075"
     CDS             2582..3100
                     /locus_tag="PKJ16_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48562.1"
                     /translation="MLAKLRDRFTETHSQYYPLVFSAVHTKVDNVDDAHDICQEVFIK
                     LYEKFDSVENPRKWLYGALRLAVFEYYRRRDPGTDIDDVFNDVSLTFVNGFRDARIVI
                     AEAMEDMVHFDGEEDKTLFDLIAVHNFSYSQTAKQLGLTKRMVEYRYRRIVESILDAL
                     KKKGVGHIEDLL"
     gene            3097..3843
                     /locus_tag="PKJ16_16080"
     CDS             3097..3843
                     /locus_tag="PKJ16_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48563.1"
                     /translation="MNSSIRSFERILERFRFREPVPDSVQAYILSTKRRAAIEVLKEV
                     ENYSFAYGMALLVYYSSRRMGIRPTIVQSKIIAAALMLIALFSIAFVAWLFVPETAPE
                     RGAPPRSRITSTATATASSPKVQTDEKRLDEKTAPSVRYRLGVETFTSDVVTSSERKR
                     IADKITNELVRLAGPDRVVRIGVVGRKNVNRVLLGAIEQLGDTYIITAKIVDVEKSRV
                     LFSTSESAGTEQEIDSACERIARKAAGHVE"
     gene            3873..4889
                     /locus_tag="PKJ16_16085"
     CDS             3873..4889
                     /locus_tag="PKJ16_16085"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV48564.1"
                     /translation="MKRIQHSRLLIFLEIFTILLISGIIAPIVAADKQQHKKRVAILD
                     FTAENTTKTYANAVRNLFEVEIHKTAVFDIVERNQMETILNEQGMQMTGCIDTACAVQ
                     IGKMLSAELIVTGSVSKMDGYTITVKFVNVGKGKIEFADSTAARTDRELQQAVATLAG
                     KIAKAEKTSTASHGEGRGFSISLIGGPSRHMGEKAEYLKPTIVYGLLLGYDFLCSSHL
                     NLSGVIHGFHLAHRESSEFPKKMELALSGGHGGIGFGLTLADTLRFQINVLAGYASSE
                     LKSGVDGKKYSSGDMSLMALFETRLYLFKGVYVLGFASYQAVLYSGSPLQEMQYGGGL
                     GYVF"
     gene            4951..>5209
                     /locus_tag="PKJ16_16090"
     CDS             4951..>5209
                     /locus_tag="PKJ16_16090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48565.1"
                     /translation="MKTLTHKRLQIRNALHNMLGHKTSYNSVLQYALMLLATSLFFTC
                     AGDDILGKYENPASTSTRNTNLSWVLMFQDDFDGDGIWQNIG"
BASE COUNT         1225 a         1441 c         1403 g         1140 t
ORIGIN      
        1 gctggtcttc aggcttgagg atggtcgcga ccgggcccat ctcggtctgg ccgtagtagt
       61 tccacaggcg caggccgtga aaggtgatgg agagctgctt gatgatctcc accggcatga
      121 tgctcgcgcc gtaggctgcc ttcttgaggc tcgcgtggtt gtacttcttg aactccgggt
      181 ggttgatgat gccgatccag accgtgggcg gcgcgaacat gtgcgttatc tgaaacttct
      241 cgatcgactt gatcatctcc accgggtcgg cgcggtgcag gatcacgttg gtggccccca
      301 cgtagatgaa cggcagcagg aagcagtgca gctgcgcgca gtggaacagc ggcagcgcgt
      361 gaaggctcac gtcctcggtc tggtactcgc cgtcgaagat gcagctgtag tactgcgaca
      421 tgagcgcgcg gtgcgagagc atcgcgccct tgggcttcga ctccgtcccg ctggtgtagg
      481 ggatctgcac gatgtcctcg gcctccacgt caacctgcgg ctcgtcgtac ggcattgact
      541 caagctcggc catgagattg aagaacccct ccggaaccac ggtgtcggcc agcgggagga
      601 agccccattt cccgaccgtc gtgaactttc cctgcgtttg cgcctgggcg atcaccgggt
      661 agagcgcgtc ctcgacgatg aacaccttcg cgccggagtg gttcacgatg taggcgatct
      721 cctcggcgtt gagcatgtag ttgatgggca cctgcacggc gccgatcttc ccgagaccga
      781 tgaggctgat ggggtaatag tgcgaattgt acgccaggat cgcgacccgg tcgcccttct
      841 tcacgccgta gctcgccatg aggtgcgcga agcggcgcga ctcccgctcg agatcataaa
      901 acgagagcgt cgtgtcgcgg aagatgatcg cggtcttgtc gccgtacttg gttgcggcgc
      961 ggcgcgccat gtcgccgatg gtgtcgcggt tgatgatgtt tgccattgga ttcacctcgt
     1021 tggtcaatga tgatgtacga tgcagtgatg caaccagttc accgaacgag cgctcggttg
     1081 gcaatgaaat atttttcatg gacaaaaaaa tattatttgt ccaccgattt atacaaaaaa
     1141 ccgattttat atttaaatcc acaccttccc attcattaca acctttttag agattgacac
     1201 tgtatgcgat tgtggcacga atcataattg ttacgtgctg cacctttgcc tacacacgga
     1261 tccgtgactt tcttttgaga ggtcaaaaga aagtcaccaa agaaaaacct ctcgctccaa
     1321 ggccgcgagg ggccaaatag acttcgtatg atgataccac atgcctttgt tagagaacgg
     1381 cttgcactat ccctcggtct ccgactgcgc gaaaacctgc gacctcggcc ttatcgtttc
     1441 agactgttga aaaaccccat gcactggatt cacatattct gcaatgactc aatcatgcgt
     1501 agacgtaaaa tattcatcag caatgaattc tgagccagcc tatctcaaaa gccacacaac
     1561 tggacgactt aaggatatca ccgatgagct gcgcggggtg atccgcgcct gcgcgctctg
     1621 cccgcatcgc tgcggcgtgg accgcacgat tggagaacgc ggaaaatgcc gcagcggcgc
     1681 gctgcccatc gtctcctcgt ttcacgcgca cttcggagag gagcgcccgc tcgtggggcg
     1741 cggcggatcg ggcgccatct tcttcaccaa ctgcaacctc gcctgcatct tctgccagaa
     1801 ctacgacatc tcgcactacg gccgcggcga cgaggtttcg ttcgatgagc tcgccgccat
     1861 gatgctcgcc ctgcaggcga agggctgcca caacatcaat ttcgtgtcgc cctcgcacat
     1921 gctgtattcg atcgtcgagg cgcttctcat cgcggtgccg cggggactct ccctgccgct
     1981 cgtgtacaac accggcggtt acgattccgc caacatcatc catttgatcg acggcgtctt
     2041 cgacatctac atgccggact tcaaattcat ggacgcgtcc gtcgcgaacg aactctccga
     2101 cgcgccagac tacccggagc gcgcgatgga ggctgtcgcc gagatgcacc gccaggtagg
     2161 cgacctcgag ctcgacgatc gcggcatcgc ccgccgcggc ctgctcgtcc gccatctcgt
     2221 gctgcccaac aacatcgcgc ggaccgacgc ggtcctcgat tttctggcgg gactctcgcg
     2281 ggacacctac gtgaatatca tggaccagta ccgccccgag taccgcgcac gggagtgctt
     2341 cgatctgcgg cggcgcgtca ccctccagga attcgacgcg gccgtggcgt gggcgcggaa
     2401 ggcgggtctg cggcggatcg atggggtgtg atgcatgacc atgcttgacg catttcgtaa
     2461 gaaaaacccc atcagaccgc cgcatcgcct atattttgct tgcctcgaca ccgcatggtc
     2521 ccatgcgctt attcccgtgt gtcgaaatcc aacgaatgat cgcttataaa cataccgaca
     2581 gatgctggca aaactgagag acaggttcac cgaaacgcac agccagtatt acccgctggt
     2641 gttcagcgcc gtgcacacga aggtggacaa tgtggacgac gcccacgaca tctgccagga
     2701 ggtgtttatc aagctgtatg agaaattcga ttcggtggag aacccgcgca aatggctcta
     2761 tggggcgctg cggctcgccg tgttcgaata ctatcgacgc cgggacccgg gcacggacat
     2821 tgacgacgtc ttcaacgacg tgagcctcac cttcgtgaac ggcttccgcg acgcgcgtat
     2881 cgtgatcgct gaagcgatgg aggacatggt ccacttcgac ggagaagagg acaagaccct
     2941 ctttgacctc atcgccgtcc acaacttcag ctatagccaa accgccaaac agctcggcct
     3001 caccaagcgg atggtcgagt accggtatcg tcgtattgtt gaaagcatcc ttgatgcgct
     3061 caagaaaaag ggtgtcggcc atatcgagga cctgctatga acagctcgat acggtcattc
     3121 gaacgtatat tggaacgctt ccggttccgg gagccggtgc ccgactccgt tcaggcgtac
     3181 attctatcaa caaaacgacg ggccgccatc gaggttctaa aagaagttga aaactattcc
     3241 ttcgcatacg gcatggcgct tttagtctat tattcctcac gccgcatggg catacgtcca
     3301 acgattgtgc aaagtaagat catagctgcc gcgctcatgc tcatagcgct gttttcaata
     3361 gcgttcgtcg catggctttt tgtgcctgaa acagcgccgg aacgcggagc accgccccga
     3421 tccaggatca cgtcaacagc taccgcgact gcatcttcgc ctaaagtcca aacggacgaa
     3481 aaaaggcttg atgagaaaac ggccccctcg gtacgatacc gactgggcgt tgagacgttt
     3541 acgagcgatg tagtcaccag tagcgaaaga aaaagaatag cggataaaat aacaaacgaa
     3601 cttgtacggc tggctggccc cgaccgggtg gtacggatcg gtgttgttgg caggaaaaat
     3661 gtcaaccggg ttcttcttgg cgcaatagag caactcgggg acacgtacat cattaccgcg
     3721 aaaatcgtgg atgttgagaa atcgagggtg ctgttttcca cgtccgaaag cgcgggaaca
     3781 gagcaggaga tcgattcggc atgtgagcgt atagcccgca aggcggccgg tcacgtcgaa
     3841 tgatttcact tcatcatcca ttggagattc cgatgaagcg catacaacac agccgactgt
     3901 taatctttct tgaaattttc acaattcttc ttatttccgg catcattgct cccattgttg
     3961 ctgcggacaa acaacagcac aaaaaacggg ttgccattct cgattttacc gccgaaaaca
     4021 ccacgaaaac ctacgcaaat gcagtgcgga acctgttcga ggtcgaaata cacaagacgg
     4081 ccgttttcga tattgtcgaa cgaaaccaga tggaaacgat tttaaacgaa cagggaatgc
     4141 agatgaccgg ctgcatcgac accgcatgcg ccgtgcagat aggaaaaatg ctttcggcgg
     4201 agctgatcgt caccggcagc gtaagcaaaa tggacggata cacaattacg gtcaagtttg
     4261 tcaatgtcgg taagggtaaa atcgaatttg ccgattccac ggcagcgaga acagaccgag
     4321 aactccaaca ggccgtcgcc accctcgccg gcaaaatcgc aaaagcggag aaaacgagca
     4381 cagcctctca cggcgaagga agggggtttt ccatttcgct catcggcggc ccctcacgcc
     4441 acatggggga gaaagcggag tacctgaaac cgacgatcgt atacggcctt cttctcggat
     4501 atgattttct ctgttcaagt catctcaatc tctctggcgt aatacatggt tttcacctcg
     4561 cccaccggga atcatcggaa ttcccgaaga aaatggaact ggctctttcg ggcggacacg
     4621 gagggatcgg ttttggccta acgctcgccg acacgttacg atttcagatc aatgtgctcg
     4681 ccggatacgc atcatcagaa ctaaaaagcg gggtggatgg aaaaaaatac agctccggcg
     4741 acatgagcct gatggctctg ttcgaaacac gtctttacct gtttaaaggg gtatacgttc
     4801 tgggtttcgc atcctaccaa gctgttctgt actcgggctc tccgctacag gagatgcagt
     4861 acggaggggg tttggggtat gtgttttagt atttttgtca aattcattcg aattgtctcg
     4921 catgaaacaa caaagaggaa atcagtaacg atgaaaacct taacccataa acgtttgcaa
     4981 atccgtaatg ctctacataa tatgcttgga cacaagacct cctacaacag cgtgcttcaa
     5041 tatgcactaa tgctcttagc gacatctcta tttttcacct gtgccgggga cgacatattg
     5101 gggaaatatg aaaatcccgc atcaacttca acgaggaata ccaacctatc ctgggtgtta
     5161 atgtttcaag atgatttcga cggcgacggc atatggcaaa acatcggca
//
LOCUS       DAORHF010000258         5199 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16589_length_5199_cov_4.430404, whole genome shotgun sequence.
ACCESSION   DAORHF010000258 DAORHF010000000
VERSION     DAORHF010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16589_length_5199_cov_4.430404"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKJ16_16095"
     CDS             <1..551
                     /locus_tag="PKJ16_16095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48566.1"
                     /translation="VMLGYYKNKEATKEVMTKDGFFKTGDIGIIDEKGRLSITGRIKD
                     IIVTAGGKNISPQNIENSLKESKFIEQCAIIGDKRKYLSALVIPAFEAVQKWAKAQGI
                     SFSSNDDLIKNQQVIDLIGKELDTYTKQFARVEQIRKFRLLNAEWTQATDELTPTLKV
                     KRRVIEKKYAAEIESMYPPDEQ"
     gene            823..2640
                     /locus_tag="PKJ16_16100"
     CDS             823..2640
                     /locus_tag="PKJ16_16100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNV48567.1"
                     /translation="MPTVTENSMAMVFKNQVAKLGDKPCAGYKKDGVYYYLSWNEMDE
                     KVRNLGYYLMSIGVKKGDRVALFSPNRYEWWVADLAILSIGAVNVPIYATNSSEESHY
                     VLDHSESKVCLVGTESHLDRVLAVKKRLKKLKTIIIFDKPKKPRAGVLTLQQAYDAGA
                     RYKNKNEFDKRLNAIKRSDMATIIYTSGTTGNPKGVMLSHGNFIANVDQIREDFESYL
                     EQDLHFMSFLPLSHSLERTAGYYLAIGAGARVSFADDFSTLMTDMQLARPTIIISVPR
                     LYEKIHAGILSKAAEVTGVKKALFNFAMSTSAKNMPYVCENRPRTGLFAVRYAIADKL
                     ILSKIKAALGLDKLILAISGGGPLSVSDAEFFLGMGLVILEGFGLTETTPVTNVNRPG
                     EIKPGSVGRPMKDTTIKISDEGEILIKGPQVMLGYYRNKKATEEAFTKDGFFRTGDIG
                     IFDDRGRMSITGRIKDIIVTAGGKNISPQNIENTLKGSKFIEQVAVIGDKRKYLSALV
                     IPAFEEVKKWATKQGLRFNSNDEMIANDAVHALIAKEIEMYMKDFARVEQIRKFRLLN
                     AEWTQATDELTPTLKVKRRVIEKKYAPEIESMYPADSGD"
     gene            complement(2722..4158)
                     /gene="ahcY"
                     /locus_tag="PKJ16_16105"
     CDS             complement(2722..4158)
                     /gene="ahcY"
                     /locus_tag="PKJ16_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HNV48568.1"
                     /translation="MSTTKTVQKLDTSLPYKVADISLAEWGRKEMELAENEMPGLMSV
                     REKYGPQKPLKGLKVTGSLHMTIQTAMLIETLKELGADIRWASCNIFSTQDHAAAAIA
                     KAGTAAVFAWKGETLEEYWWCTEQALTWPDGSGPDLIVDDGGDATLMVHQGVKVEKDP
                     SILSQKFDNPEFRIIMDRIADGYTSSKSRWTDMAKKIRGVSEETTTGVHRLYQMFEKG
                     ELLFPAVNVNDSVTKSKFDNLYGCRESLADGIKRATDIMMAGKIVVVCGYGDVGKGCA
                     QSMKGLGARVIITEIDPICALQAAMEGYQVSTMDEMASVGDIFVTATGCFDVITGEHM
                     EKMKDEAIICNIGHFDSEIQMSYLENTVGCKKITIKPQVDRWMLKSGRSIIVLAEGRL
                     VNLGCATGHPSFVMSNSFTNQCLAQIHLASNTLEPRVYTLSKKLDEEVAALHLGKLGA
                     KLTKLTKEQADYLGIPVDGPYKPEHYRY"
     gene            complement(4180..5112)
                     /locus_tag="PKJ16_16110"
     CDS             complement(4180..5112)
                     /locus_tag="PKJ16_16110"
                     /inference="COORDINATES: protein motif:HMM:NF017001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV48569.1"
                     /translation="MELIKSLKALGDDTRLRIVNILSQYELQVNEIVDVLDMGQPRIS
                     RHLKILADHDLLTARRDGLWAYYSVARGNGHSALIDTIIQHLSADPDPHYRNDLNRAA
                     QVLDERKNRTRSFFNTIAGEWEDLKKNIFGDFDIVRAILDELPAITSVADLGCGTGDL
                     IPALRDRASCVIGVDSSPAMIELARKKLSALGDAVDLRLGELEHLPIGNGEVDAVIIN
                     MVLHHVSQPPAALREAYRIVKRGGLCIITDFEKHADEHVRQAYGDRWLGFDREQISSW
                     SKDAGFVVEKTNRFPVRGGLSIQCFVNRSNVVSN"
BASE COUNT         1176 a         1420 c         1467 g         1136 t
ORIGIN      
        1 aggtgatgct tggctattac aagaacaagg aagccacgaa agaggtgatg acgaaggacg
       61 gtttcttcaa aaccggcgat atcggcatca tcgacgaaaa gggccggctc tcgatcaccg
      121 ggcggatcaa ggacattatc gtgaccgccg gcgggaagaa tatttccccg cagaacatcg
      181 agaacagcct caaagaatcg aagttcatcg aacagtgcgc catcatcggc gacaagcgga
      241 agtatctctc ggcgctggtc atccccgcgt tcgaagcagt tcaaaagtgg gcgaaggcgc
      301 agggaatatc gttctccagc aatgacgatc tcatcaagaa ccagcaggtg atcgatctga
      361 tcggcaagga gctcgatacg tacacgaagc aattcgcgcg tgtggagcag atacgcaagt
      421 tccgcctcct gaacgccgag tggacgcagg ccaccgatga gctcactccg acccttaagg
      481 tgaaacgtcg cgtgatcgag aagaaatatg cggcggaaat cgaaagcatg tatccgccgg
      541 acgaacaata agcggtcttc tatacctggt tgccaaatgg ggcgggaaac acccgcccca
      601 ttttttttat catcccgcct ttgagttctt gcgtaaatat ttcatgtttg cactatgggt
      661 cgtatgagag accggacaat cggtctgcga ataaatgtcg acactgtgcg atttgtgtgc
      721 atatttcgac tgtgatcgat cgagacggat ttgcggcgcg acgtggagcc gggagcgttt
      781 ccaggctctt attatagtat cgatcttcaa aaggagtttg tcatgccaac agtaacggaa
      841 aactcgatgg cgatggtgtt caagaaccag gtggcgaagc tcggagacaa gccgtgcgcc
      901 ggatacaaga aagacggcgt gtattattac ttgtcatgga acgagatgga cgaaaaggtg
      961 cgtaatctcg gttactacct catgtccata ggcgtcaaga agggcgatcg cgtcgcgctg
     1021 ttttcgccga atcgatatga atggtgggtt gccgatttgg cgatcctctc gatcggcgcc
     1081 gtcaatgtac ccatttacgc gacgaattca tcagaggaat cgcattacgt tctcgaccat
     1141 tccgaatcga aggtctgcct tgtcggaacg gagagccacc ttgaccgcgt tttggccgtg
     1201 aaaaagcgcc tgaagaaact gaagacgata ataatctttg ataagccgaa aaaaccgcga
     1261 gcgggcgtac ttacccttca gcaggcatac gacgcgggcg ctcgctacaa gaataaaaac
     1321 gaattcgaca agcgtttgaa tgcgatcaag cgatcggaca tggcaaccat tatttacacc
     1381 tccggaacca ccgggaatcc caagggcgtt atgctctccc atggtaattt catcgccaat
     1441 gtagaccaga ttcgcgagga cttcgagagc tatctcgagc aggacctgca cttcatgtcg
     1501 ttcctgccgc tttcgcactc gctcgaacgg acggcggggt actatctcgc gattggggcc
     1561 ggcgcgcgcg tatcgttcgc cgacgatttc tcgacgctca tgaccgacat gcagcttgcc
     1621 cgtccgacca tcatcatcag cgtaccgcgc ctgtatgaga agatccacgc gggaattctt
     1681 tcaaaggccg ccgaggtgac cggcgttaaa aaggcgctct tcaatttcgc catgtcaaca
     1741 tcggcgaaaa acatgcccta cgtctgcgag aaccgtcccc ggaccgggct gtttgccgtt
     1801 cgatacgcca tcgcggacaa actcattctc tcgaaaatca aggctgcgct cggactcgac
     1861 aagctgatat tggccatctc gggcggcggg ccgctgtcag tgtcagatgc cgagtttttc
     1921 ctggggatgg ggctggtgat tctcgaggga tttggcctga cggaaaccac gccggttacg
     1981 aacgtgaacc gaccgggcga gatcaagccg ggttcggtcg gccggccgat gaaggacacc
     2041 acgattaaaa tctccgacga gggtgagata ctcatcaagg gcccgcaggt aatgctgggc
     2101 tactatcgga acaaaaaggc gacggaggag gccttcacta aggacggctt ctttcgaacc
     2161 ggggacatcg gcatattcga cgatcgcggc cggatgtcga tcaccgggcg aattaaggac
     2221 atcatcgtga ccgccggcgg gaaaaacatc tcaccgcaga atatcgagaa cacgctgaag
     2281 ggatcgaagt tcatcgaaca ggtcgccgtc atcggcgaca agcgaaagta cctctcggcg
     2341 cttgtcattc ctgcgttcga ggaggtgaag aagtgggcga cgaaacaggg gcttcgattc
     2401 aactcgaatg acgagatgat agcgaacgat gcggtacacg cgcttatcgc gaaggaaatc
     2461 gaaatgtaca tgaaggattt cgcgcgcgtg gagcagattc gaaaatttcg tctcctgaac
     2521 gccgaatgga cgcaagccac cgatgagctc accccgaccc tcaaggtgaa acgtcgcgtg
     2581 atcgagaaga aatacgcgcc cgaaatcgaa agcatgtatc cggcagattc gggggattga
     2641 aaaaggcgtt gattcggtgt cgaaccgaat agtgcgaaat gtcgatccgc cggtcgtgtt
     2701 tacgaccggc ggatctgttt actagtagcg ataatgttcc ggcttgtacg ggccatcgac
     2761 agggatgccc aggtaatcgg cctgttcttt ggtgagcttg gtgagctttg cgcccagctt
     2821 gcccaggtga agcgctgcca cctcctcgtc gagcttcttg gagagcgtgt ataccctcgg
     2881 ctcgagcgta ttggacgcaa ggtggatctg cgcaaggcac tggttggtga agctgttgct
     2941 catcacgaaa ctcggatggc cggtcgcgca cccaaggttc accaggcggc cttccgccag
     3001 cacaatgatg gacctgccgg acttgagcat ccatcggtcc acctgcggtt tgatggttat
     3061 cttcttgcat ccgaccgtat tctcgagata cgacatctgg atctcggaat cgaaatgccc
     3121 gatattgcag atgatcgcct cgtccttcat cttctccatg tgctcgccgg tgatcacgtc
     3181 gaaacagccg gtggccgtga caaagatgtc gccgaccgac gccatctcgt ccatcgtgga
     3241 cacctggtag ccctccatcg ccgcctgcag cgcgcagatg ggatcgattt cagtgatgat
     3301 cacgcgggcg cccaggccct tcatcgattg cgcgcacccc ttgccgacat cgccatagcc
     3361 gcacaccacg acgatcttgc cagccatcat gatgtcggtc gcgcgcttga tgccgtcggc
     3421 cagcgactca cgacagccgt agaggttgtc gaacttcgat ttcgtaacgg agtcgttgac
     3481 gttcaccgcg gggaagagca gctcgccctt ttcgaacatt tgatagaggc gatgcacgcc
     3541 cgtggtggtt tcctcggaca cgccgcggat ttttttcgcc atgtcggtcc agcggctctt
     3601 ggaactcgta tacccgtccg cgatgcggtc catgatgatc cggaactcgg gattgtcgaa
     3661 tttttgcgaa aggatcgacg ggtccttctc gaccttgacg ccctgatgca ccatgagcgt
     3721 cgcgtcgccg ccgtcatcaa cgatgaggtc ggggccgctg ccgtcgggcc aggtgagggc
     3781 ctgttcggtg caccaccagt actcctcgag ggtctcgccc ttccatgcga acaccgcggc
     3841 cgtaccggcc ttcgcgatcg ccgccgccgc atggtcctgc gtcgaaaaga tattgcacga
     3901 tgcccagcgg atatcggcgc ccagctcctt gagcgtctcg ataagcatgg ccgtctgtat
     3961 ggtcatgtgc aggcttccgg tgaccttgag gcccttgagc ggtttctgcg ggccgtactt
     4021 ttcgcgcacc gacatcaggc cgggcatttc gttttccgct agctccatct ccttgcgccc
     4081 ccattccgca aggcttatat cggccacctt gtagggaagg gacgtatcca gcttctggac
     4141 ggttttcgtg gtactcattg ttcctccata aaaaaatatt taattgctca cgacgttaga
     4201 acgattgacg aagcactgaa tcgaaagacc gcctcgaacg ggaaagcggt ttgtcttttc
     4261 gacgacgaat cccgcgtcct tcgaccagga cgaaatctgt tcgcggtcga atccgagcca
     4321 gcgatcgccg taggcctgcc gtacatgctc gtcggcgtgt ttttcaaaat cggtgatgat
     4381 gcacaggccg ccccgtttca cgatccggta cgcttcgcgg agggccgccg gcggttgcga
     4441 cacgtggtgc agcaccatgt tgataatgac cgcgtccacc tcgccgttgc cgatgggaag
     4501 gtgctccagc tcgccgagac gcagatccac ggcgtcgccg agcgcggaaa gtttttttcg
     4561 cgccagctcg atcatcgcgg gcgagctgtc cacgccgatg acgcatgagg cgcgatcgcg
     4621 gagcgcagga atgaggtctc cggttccaca gccgaggtcc gcgaccgatg tgatcgcggg
     4681 aagctcgtca aggatcgctc tcacgatgtc gaagtcgccg aaaatgtttt ttttcaggtc
     4741 ctcccactcg ccggcgatcg tgttaaagaa cgatcgcgtc ctgttcttgc gttcgtcgag
     4801 cacctgcgcg gctcggttga gatcgttccg atagtgcgga tcggggtcgg cggagagatg
     4861 ctgtatgatt gtatcgatga gcgcgctatg tccattgccg cgcgccaccg agtaatacgc
     4921 ccacaggccg tcccgcctgg cggtaagaag atcgtgatcg gcaaggatct tcagatggcg
     4981 tgaaatgcgg ggttgtccca tatccaggac atccacgatt tcattaacct gcagctcata
     5041 ctgcgacagg atgttcacta tccgcaatct ggtatcatca cccagcgctt ttaaggattt
     5101 tattaattcc atattactat atcaatatat actgatatag taatatggcg actgatttgt
     5161 caataaattt taaagatttc agtgcaaaat tacgaaaaa
//
LOCUS       DAORHF010000259         5123 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_16955_length_5123_cov_5.104972, whole genome shotgun sequence.
ACCESSION   DAORHF010000259 DAORHF010000000
VERSION     DAORHF010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16955_length_5123_cov_5.104972"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PKJ16_16115"
     CDS             <1..418
                     /locus_tag="PKJ16_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48570.1"
                     /translation="CIDEFRRALQFEGIFLYGDVVLAQDDGDGIYDELLRMLLPLHNE
                     FYLDEELRTVLELKDFSFVKGMLMRFPRSLPSLMDALVRSVPELSVAAALAFIEAHRD
                     AFAAHYQLDAASSIQEPYFLGYFMRNKPKKPAADAP"
     gene            complement(421..1059)
                     /locus_tag="PKJ16_16120"
     CDS             complement(421..1059)
                     /locus_tag="PKJ16_16120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48571.1"
                     /translation="MKRLLISTVALVSFAMLAQCASISITATWKDSAYTAGPFKKTVV
                     MALFNNLETRQAVEAGMVAELKRHGNDAAPSLDFMAPNQKYAHAQIEKLLEKNGIDGI
                     LIIKLMGISKKQKYHPGSNYFQPNFYYDMYNEYYVMYSMRRDPGYIDEKDYVKIRASL
                     FANSTDRMVWRAEMTAVEDYQTKDGLTNPKTEAPVLAKLVHANLVANGLAKR"
     gene            complement(1097..1648)
                     /locus_tag="PKJ16_16125"
     CDS             complement(1097..1648)
                     /locus_tag="PKJ16_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV48572.1"
                     /translation="MRARYLIIAFMLLALLAADTPQDRNTALLEAASAGNLPGIEAAL
                     KTGADINTADRIGRTPLMSAAFNGYEGAVALLIRKGCTVNARDSKGWTALILASSQGR
                     GPAVAALLAAKGIDVNARERDGRTALMWASYTGGDPGVVRALIAKGADVSLKDTKGRT
                     ALNYARGAKQTEIVGIIMAAGGK"
     gene            complement(1709..2494)
                     /locus_tag="PKJ16_16130"
     CDS             complement(1709..2494)
                     /locus_tag="PKJ16_16130"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HNV48573.1"
                     /translation="MNVKDLFDLSGKTAIVTGGGRGIGQFIAMGLAEAGANVVIASRK
                     LDKLEKAAADIRSLGVKSVPVKCDMGNKDDIDRLVEVTMKEFGTVDILVNNAGVTWGA
                     PTLDFPLEQWDKIFNVNVRGVWILSQSVAKIMKEKGGGKMINISSVMGFRGSIEEAHP
                     AVPYNSSKAAINLLTMNLAIKLARYKIYVNAIAPGFFHTDMMGYLDKEEMKPLRDAML
                     MTIPIPKMGEADDIKGLAVFLASRASDYVTGVVIPVDGGMLAK"
     gene            complement(2828..3841)
                     /locus_tag="PKJ16_16135"
     CDS             complement(2828..3841)
                     /locus_tag="PKJ16_16135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HNV48574.1"
                     /translation="MLDHLKAIMRNDSVLLKPEAAATRPRAEALRRAGRAVAVFFVRI
                     KDFFIRDLPRAFKNFNLHDGELSTCAIAFFLLISFIPVSLVIISILSFFFDSGSLAAS
                     TYLVQIKMLLPSIQINELITRIDKIVFQKRYLAFVWLPFLFWWGSLVFDTVERALEIA
                     FRIERNRKYWKAKIRHFIIIIGISALAVVITLISNLAAVAKNADITLLIEQSTRTLPA
                     LKAKLDILIRTPLIFSSLAALAINTFLVFMLYRFIPPKKIDNLSMFKGALFVSFSYEV
                     VKILFSYYITQINDYTSIFGSLSSIVILMIWIWYTCFLFVFGAEVSWVFYERGRDIEH
                     AHP"
     gene            complement(3856..4554)
                     /locus_tag="PKJ16_16140"
     CDS             complement(3856..4554)
                     /locus_tag="PKJ16_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012502526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HNV48575.1"
                     /translation="MFLGIDPLYWMMMAPVMALSLWASFRVKSTFTRFSQVESRSGMT
                     GAEAAAAILHRNGLDDVEVVETGGFLSDHYDPGRRVIRLSTDVFHGSSISSIGVAAHE
                     TGHALQHAHRYGPLVLRNTMAPVAQISSWASWAIIVGGFILNWLDLVKIGIVVFSVVV
                     AFQVITLPVEFNASTRAKEALASYGIVGRNELAGVDRVLSAAAMTYVAAAASAIVTLI
                     YYLLRAGILGRKDD"
     gene            4793..>5123
                     /locus_tag="PKJ16_16145"
     CDS             4793..>5123
                     /locus_tag="PKJ16_16145"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011011541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HNV48576.1"
                     /translation="MAQIGIILGSDSDLPKIKDCFAILEEFGVSFEVIVSSAHRTPAQ
                     THEWASTARERGLRAIIAAAGGAAHLPGVVAAFTTLPVIGVPIETNVAGGLDSILSII
                     QMPAGVPV"
BASE COUNT          947 a         1537 c         1623 g         1016 t
ORIGIN      
        1 gtgcatcgac gagttccgcc gggcgctgca gttcgagggg atattcctct acggcgacgt
       61 ggtgctggcg caggatgacg gcgacgggat ctacgacgag ctgttacgga tgctgctgcc
      121 gcttcacaac gagttctatc tcgacgagga gctgcgcacg gtgctcgagc tcaaggattt
      181 ttcattcgta aagggaatgc tcatgcgctt cccgcgaagc cttccgtcgc tgatggacgc
      241 gctcgtgaga agcgttccgg agttgtccgt cgcagcggcg ctcgccttca tcgaagcgca
      301 ccgcgacgca ttcgccgcgc actatcagct cgatgcggcg tcttccatcc aggagccgta
      361 tttcctgggc tatttcatgc gcaacaagcc caagaagccc gccgccgacg cgccctgacg
      421 ctatcgcttc gccaggccgt tcgcgacgag gtttgcgtgc acgagtttag cgagcaccgg
      481 ggcctcggtc ttgggattgg tgaggccgtc cttcgtctgg tagtcctcca ccgcggtcat
      541 ctccgcccgc cataccattc ggtcggtgct gttcgcgaag agcgaggcgc ggatcttcac
      601 gtagtccttt tcgtcgatgt agcctggatc gcgccgcatg gagtacatga cgtagtattc
      661 gttgtacatg tcgtagtaga agttcggctg gaagtagttg gaacccgggt ggtatttctg
      721 cttcttcgaa atccccatga gtttgatgat gaggatcccg tcgatgccgt ttttctcgag
      781 gagcttctcg atctgcgcat gcgcgtactt ctgattgggc gccatgaaat cgaggctcgg
      841 ggcggcgtcg ttcccgtgtc gcttgagttc ggccaccatg cccgcttcga ccgcctgccg
      901 cgtctcgaga ttgttgaaca gcgccatgac cacggttttc ttgaacggac ccgcggtata
      961 ggccgagtct ttccaggtcg cggtgatgga gatggacgcg cactgcgcga gcatcgcaaa
     1021 ggaaacgagt gcgacggttg atatcaaaag gcgtttcatg caatcctccg ggagtgtgtg
     1081 cttgtgatta tggacgtcat tttcctccgg ccgccatgat gatcccgacg atctcggtct
     1141 gcttcgcgcc acgcgcataa ttgagcgctg tgcgcccctt ggtgtccttc agcgatacgt
     1201 cggcgccttt cgcgatgagg gccctgacca cgcccggatc gcctcccgtg tatgacgccc
     1261 acatcaacgc ggtgcggccg tcccgctcgc gggcattgac gtcgatcccc ttcgccgcga
     1321 gaagagccgc aaccgccggg ccacgcccct gcgatgatgc gaggatgagc gcggtccatc
     1381 ccttgctgtc gcgcgcgttg acggtgcatc ccttccggat gagcagcgcg accgcgccct
     1441 cgtacccgtt gaacgccgcc gacatgagcg gcgtcctgcc gatcctgtcg gcggtgttga
     1501 tgtctgcgcc ggtcttgagc gcggcctcga tccccgggag gtttcccgct gatgcggctt
     1561 cgagcagcgc ggtgtttcgg tcctgcggcg tgtcggcggc gagaagcgcc aacagcatga
     1621 acgcgatgat gagataacgt gcgcgcatgt gtgttcctcc gaatgatcgc tttggatgaa
     1681 ccgcagccct gcggggcgcg gatcggccct atttcgcgag catgccgccg tccaccggaa
     1741 tgacgacgcc ggtcacgtag tcggacgcgc gcgaggccag gaacaccgcg aggcccttga
     1801 tgtcgtccgc ctcgcccatt ttcgggatcg ggatggtcat gagcatcgcg tcgcgcagcg
     1861 gcttcatctc ctccttgtcg aggtagccca tcatgtcggt gtggaaaaag cccggcgcga
     1921 tcgcgttcac gtaaatcttg tagcgcgcga gcttgatggc gaggttcatc gtcaggagat
     1981 tgatggccgc cttcgaggaa ttgtacggca cggcgggatg cgcctcctcg atggagccgc
     2041 ggaagcccat caccgacgag atgttgatca tctttccgcc gcccttttcc ttcatgatct
     2101 tcgcgacgga ctgggagagg atccaaacgc cgcgcacgtt cacgttgaaa atcttgtccc
     2161 actgctcgag cggaaagtcg agcgtgggcg cgccccaggt gacgccggcg ttgttcacca
     2221 ggatgtccac ggtgccgaac tccttcatgg tgacctcaac caggcggtcg atgtcgtcct
     2281 tgttgcccat gtcgcatttc accggaacgc tttttacgcc aagcgaacgg atgtccgccg
     2341 cggccttctc gagcttgtcg agtttgcgcg acgcgatcac cacattggcg ccggcctctg
     2401 cgagccccat ggcgataaac tggccgatac cgcgcccacc gccggtgacg atggccgtct
     2461 tccccgagag atcaaaaagg tccttcacgt tcatgttcgc ctcctgtggt tgttgccgtc
     2521 gatgatatca tcatccccct ttcgctgcgc gttttgagaa atcctcggag cgcgatgccg
     2581 ccacgtgcgg cgggaccggt ccgtatgacg cggagcgtgg gggcgcggtg taccgatgcg
     2641 cacctgttgc cagcagggca cgaaagggta aaaagtcaag ccgattaccc gtaggaatcg
     2701 cggcatatcc atgatgagcg tgggccgatg tgtctgcgtc cgcgatgcgc tcccgcatgg
     2761 caaatatcag gcgcgcgcga accagtcgcc tgcgtgctgc ggacacgtgt gttgatgaat
     2821 cgcgcggtca gggatgcgcg tgctcgatat cgcgcccgcg ctcgtagaag acccacgata
     2881 cctccgcccc gaagacgaac aggaagcagg tgtaccagat ccagatcatg aggatgacga
     2941 tcgaggagag cgagccgaag atggaggtgt aatcgttgat ctgcgtgatg taatacgaga
     3001 acaggatctt cacgacctcg tagctgaacg agacgaagag cgcgcccttg aacatggaca
     3061 ggttgtcgat ttttttgggc gggatgaagc gatagagcat gaacaccaga aaggtgttga
     3121 tggcgagcgc cgcgagcgac gagaagatga gcggcgtcct gatgaggatg tcgagcttcg
     3181 ccttgagggc gggaagggtg cgcgtgctct gctcgatgag cagcgtgatg tccgcgtttt
     3241 tcgcgaccgc ggcgaggttc gagatgagcg taatgaccac cgcgagcgcg ctgatgccga
     3301 tgatgatgat gaagtgccgg atcttcgcct tccagtactt gcggttgcgc tcgatgcgaa
     3361 aggcgatctc gagcgcgcgc tccacggtgt cgaataccaa gctcccccac cagaagagga
     3421 agggcagcca cacgaaggcc aggtagcgct tctggaacac gatcttgtcg atgcgggtga
     3481 tgagctcgtt gatctgtatg gatggcagga gcatcttgat ctgcaccagg taggtgctcg
     3541 cggcgagcga cccggaatcg aaaaaaaagc tcaggatgga gatgatgacc agggagaccg
     3601 gaatgaatga gatgagcagg aaaaacgcga tggcgcaggt cgagagctcg ccgtcgtgca
     3661 ggttgaagtt cttgaacgcc cgggggaggt ctcgaatgaa aaaatctttt atccgcacga
     3721 agaagacagc gacggccctg cctgcgcggc gcagcgcctc cgcgcggggt cgcgtcgccg
     3781 ccgcttccgg tttcaaaaga acgctgtcgt ttcgcatgat ggccttgagg tggtcgagca
     3841 tcgcgtcccc ggggatcagt cgtccttcct gcccagaatc cccgcgcgca gcaggtaata
     3901 gatgagcgta acgatggccg atgccgccgc cgccacgtag gtcatcgccg cggcggagag
     3961 gacccggtcc acgcccgcga gctcatttcg ccccacgatg ccgtaggatg ccagcgcctc
     4021 cttggcgcgc gtcgaggcgt tgaactcgac cgggagcgtg atcacctgaa aagcgaccac
     4081 gacgctgaag acgacgatcc cgatcttcac cagatcgagc cagttgagaa tgaagccgcc
     4141 gacaatgatg gcccacgacg cccacgagct gatctgcgcg accggcgcca tcgtgttgcg
     4201 cagcaccagc ggtccgtagc ggtgcgcgtg ctggagcgcg tggcccgtct cgtgcgccgc
     4261 gacgccgatc gacgagatcg agctgccgtg aaacacgtcg gtcgagagcc ggatgacgcg
     4321 cctgccggga tcgtagtggt ccgagaggaa gccgccggtc tcgacgacct cgacatcgtc
     4381 gagaccgttt cggtgcagga tcgccgcggc ggcctccgcg ccggtcatgc cggagcgcga
     4441 ctccacctgc gagaagcggg tgaaggtgct cttcacgcgg aatgacgccc acagcgacag
     4501 cgccatgacg ggcgccatca tcatccaata gagcgggtcg attccaagaa acatgcggtc
     4561 cctcccgacg gggtgcgatc gtattcagcg attgtgcgca ccatagctcc gaaacagatg
     4621 cgcgcgaaat gttaccacat tcactcgagc ctcaacgtag cccggcaagc cgcatatttc
     4681 aatcattttt ttggggctga tgggcgaccg tccttcgcat ttttttgttg accgacgcat
     4741 gcgggcacgc cgcagtatca tgccatcacg acgcaatcac cgggaggcga caatggccca
     4801 gatcggaatc atcctcggca gcgattcgga cctgccgaaa attaaagact gtttcgccat
     4861 cctcgaggag ttcggcgttt ccttcgaggt catcgtctcg tcggcgcacc gcacgccggc
     4921 tcagacgcac gagtgggcat ccacggcgcg cgagcggggc ctcagggcca tcatcgccgc
     4981 ggcaggggga gcggcgcatc tgcccggcgt cgttgcggcg tttaccacgc tccccgtaat
     5041 cggcgttccc atcgagacga atgtcgccgg cgggctcgat tcgatcctgt ccatcattca
     5101 gatgccggcc ggcgtgccgg tcg
//
LOCUS       DAORHF010000260         5062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17270_length_5062_cov_6.613541, whole genome shotgun sequence.
ACCESSION   DAORHF010000260 DAORHF010000000
VERSION     DAORHF010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17270_length_5062_cov_6.613541"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="PKJ16_16150"
     CDS             complement(<1..304)
                     /locus_tag="PKJ16_16150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015846031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV48577.1"
                     /translation="MTDTARSILKHPFGYDTFRPLQEEIIRSVLSKRDTLAIMPTGGG
                     KSICYQIPALLFEGLTVVVSPLISLMKDQVDQLRGYGVPALALNSMIDPDEYRENVM"
     gene            628..>5062
                     /locus_tag="PKJ16_16155"
     CDS             628..>5062
                     /locus_tag="PKJ16_16155"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV48578.1"
                     /translation="MEHIGTILNGAYRIAEKLREDWSCQVYRAHAVSGGDAVDATIIK
                     REALSGRLEGTIRFRSEMETVRDFDHPAIVRALQAGEQFGTVYVVSARCDAPQLSGVM
                     RDAAGDVPRALAISVDLCRALAYAHGRGVLHRGIRPDAVMAGRDGAMLAGFGLAHVVE
                     CAAATSPEDRATLFGYLAPEQSGLIRKRVDERSDLYQLGVLLYHLTTGRLPITGDSVT
                     AIVHRHLTQVPEPPARVNTRLPAVLDRIIMRLLEKDPDRRYQSATGLCADLERLIGGN
                     DDFIPGLDDHASRIELGGPLIGRETEFERLTRLYERTRRGRCAVCVIDGPPGSGKTRL
                     VEEFVDHVRGVGGAIMTGRCVSQGPARPYGPLNEALAGYAAAFAAYPDERRAAIRART
                     AEEFPSLRSLVATLCPAITGVIGEGGAVEPIRAEREGARFLEVAARLFCVLADCERGL
                     ALIVEDAHWADEGTMAILAAALSARGDHPLMIVLTRRGGPGGDDGFVPGTHARAIAER
                     ISLGPFDEATHERFVARILRDEGDDVRSIARRIFPHSGGIPFLSLELIRRYLDDGALS
                     YLNGRWHADRARLASIDAGGGDRDVLLARIAGLDDDDRDILSVAALIGRSFRPELVAA
                     VSGREVARVLGAIDRAGDRALIEPDVGEAYAFMHDRIREAFCAGLPGERTRALHRAAV
                     ETLARSLSDDDDATLFEIAHHAIEAEDRALIVRWASRAAGRARARYALESAGRYLREV
                     LRHLERDGDGDARALFVSATVELGEIAIVTGRTDEGVETLRGILPRIDDDRTRATAYR
                     LLTDACYRRGDWAGCEENAARGLRLLGERLPRTRGRVVAGILRELAVHAAHVLLPAVF
                     VRASTRGTGAVPREIVGFYEPLSMSYGLNDPLRLVRCALRILNIAERRIGPSPELAKA
                     LSGYASVCMAVPLFARARRYHRRALALNESLGFEGGIAKSCELMGYFYEWSGDFTAAR
                     LSFQRSLDIYRRLGDAKEYAMVLNGLEHCHYYAGEYWDALEVNQRYRSSAALSDDAYA
                     MSAVDIYYSQIYREQGDLEAANRHAGRARDLSRDRAIPFNYCSSLVELGMNALEAGEI
                     DSARTCLEEARALHERGNFLRQYIVPVYPALAQAHLDEFLRDEGGLTSEARKRRMAGL
                     LRICRLAARKTRRWRTHHGTALRIQAQCHAIAGRHAEAAALFARSIALCRELGRRFET
                     ARGLYECGMFRAHTGDDRVSRPLLEEAYRLFTELGAQLHASRIALVLGIRGTDDRETP
                     LKRLMGRERAQAAGRIGREIGGAASEEALLDLMAARTLEMTGARGCVIYLGADGDGAP
                     VRRHERGEVSPDANAHQRALAASVYSAGEFLLDAGPPVGGCALLCAPIRGEGGVTGAC
                     CIEGPADGIAFTVEDGEFLSDIYATAAMRLQAGFGTARPARTRGAERDGVTPGTEEKL
                     NAVIAYIGQNYRDELSREGLAAMVGMSPNHLGKYFAIVTGRM"
BASE COUNT          750 a         1680 c         1830 g          802 t
ORIGIN      
        1 gcataacgtt ttcgcggtac tcgtccgggt cgatcatgct gttgagcgcg agtgcgggca
       61 cgccgtagcc gcgcaactgg tccacctggt ccttcatgag cgagatgagc ggcgatacca
      121 ccaccgtgag cccctcgaag agcaaggcgg ggatctggta acagatggac ttgccgccgc
      181 cggtgggcat gatggccagc gtatcgcgct tcgacaggac cgagcggatg atctcctcct
      241 gcaggggccg gaaggtgtcg tagccgaacg ggtgtttcaa tatggatcgt gcggtatcgg
      301 tcatgattcg cctgggcgtt acatcagttg tgtgcgtctg tattgtatac gagcgtgcgg
      361 gcgattaatc caagcaagtg cgccgtgatc ctcccccgcg cattcgagac gagcatcggt
      421 attcgcgcgg tgaagggcaa atacattttt tcggccgctc aagcgaaaga accgtgcgcg
      481 tgtcatcgcc ggccgtccgc ggatttattc tcttgacttt tcacgcgaag gggagcacac
      541 aaggggtccg gttgtcgccg catcgtccta tcgggtggcc cgcgccgcgg cagggggccg
      601 tgggtcgtcg accgtcgaaa tcgcaccatg gagcacatcg gcacaatact caacggcgcg
      661 taccggatcg cggagaagct ccgggaggat tggtcgtgcc aggtctaccg cgcgcatgcc
      721 gtatccggcg gggacgcggt cgatgcgacg atcatcaagc gcgaggcgct ctccggccgg
      781 ctggagggaa cgatccgatt ccgctccgag atggagaccg tccgcgattt cgaccatccc
      841 gccatcgtcc gcgcgctcca ggccggcgag caattcggca ccgtgtatgt ggtgagcgcg
      901 cgatgcgacg caccgcagct ctcgggcgtc atgcgcgacg ccgcgggcga cgtgccgcgc
      961 gccctcgcca taagcgtcga tctctgccgg gccctcgcgt acgcgcacgg acgcggggtg
     1021 ctccacaggg ggatcaggcc ggatgcggtc atggccggtc gggacggggc gatgcttgcg
     1081 ggcttcggcc tcgcgcatgt ggtcgagtgc gccgccgcca cctcgccgga ggaccgggcg
     1141 acgctgttcg gctatctggc ccccgagcaa agcgggttga tccgcaagcg cgtcgacgag
     1201 cgctccgatc tctatcagct cggcgtgctg ctctatcacc tgacgacggg ccggttgccc
     1261 atcacgggcg acagcgtgac cgcgatcgtc caccgccacc tcacccaggt ccccgagccc
     1321 cccgcgcgag tgaacacgcg ccttcccgcc gtgctcgacc gcatcatcat gcggctcctc
     1381 gaaaaggacc cggaccgacg gtatcagagc gccactgggc tgtgcgccga cctcgagcgg
     1441 ctgattggcg gcaatgacga ctttattccc ggcctggacg atcacgcgag ccgcattgag
     1501 ctcggcggac cgctcattgg acgcgagacg gagttcgagc ggctcacgcg gctctatgag
     1561 cgaacgcgcc gtggacggtg cgccgtgtgc gtcatcgacg gaccgccggg ctcggggaaa
     1621 acgcggctcg tcgaggaatt cgtggaccat gtgcgcggcg tggggggcgc catcatgacg
     1681 gggcggtgcg tctcccaggg gcccgccagg ccgtacggcc cgctcaacga ggcgctggcc
     1741 gggtatgccg cggccttcgc cgcctatccc gacgagcggc gggcggcgat ccgcgcgcgc
     1801 accgctgagg agttcccgag cctgcgctcc ctcgtggcga cgctgtgtcc cgcgatcacg
     1861 ggcgtcatcg gcgaaggggg tgcggtggag ccgatcaggg ccgagcgcga gggcgcgcgc
     1921 tttctcgagg tcgccgcgcg cctcttctgc gtcctggccg attgcgagcg cggcctggcg
     1981 ctgatcgtcg aggacgcgca ctgggctgac gaggggacga tggcgatcct cgccgcggcg
     2041 ctctccgccc ggggagacca tcccctgatg atcgtgctca cccggcgcgg cggtccgggg
     2101 ggcgacgacg gattcgttcc cggaacacac gcccgcgcga tcgcggagcg catcagcctc
     2161 gggccgttcg atgaggcgac gcacgagcgc ttcgtcgcgc gcatcctccg cgacgagggc
     2221 gacgatgtcc gttcgattgc gcggcgcatc ttcccgcaca gcggagggat cccgtttctg
     2281 tcgttggagc tcatcaggcg gtatttggat gatggcgcgc tgtcctatct caacggacgc
     2341 tggcatgccg accgcgcgcg gctggcgtcg atcgacgcgg gagggggcga ccgcgatgtg
     2401 ctgctggcgc ggatcgccgg gctggacgat gacgatcgcg acattctttc ggtcgccgcg
     2461 ctgatcggcc gctcattccg cccggagctc gtcgccgcgg tgagcgggcg ggaggtcgcg
     2521 cgggtgctcg gggcgatcga ccgcgccgga gatcgcgcgc tcatcgagcc cgacgtcggg
     2581 gaggcctacg cgttcatgca cgaccgcatc cgcgaggcct tctgcgcggg gctccccggc
     2641 gagcggacgc gcgccctgca ccgggcggcg gtcgaaacgc tcgcccgatc gctctccgac
     2701 gacgacgatg cgacgttgtt tgaaatcgcc catcacgcga tcgaggcgga ggaccgcgcg
     2761 ctcatcgtcc gctgggcgtc ccgcgcggcc ggtcgcgccc gcgcgcgcta cgcgctcgaa
     2821 agcgccgggc gctacctgcg cgaggtgctg cgccatctgg agcgcgacgg ggacggggac
     2881 gcgcgggcgc tcttcgtgag cgcgaccgtc gagctgggcg agatcgcgat cgtcaccgga
     2941 cggaccgacg agggcgtcga gacgttgcgc ggcatccttc cgcggatcga cgacgaccgc
     3001 acgcgcgcga cggcctatcg cctgctcacc gacgcctgct accgacgcgg ggactgggcc
     3061 ggctgcgagg agaacgccgc gcgcggcctg cgcctgctgg gggagcggct cccgcggacc
     3121 cgcgggcggg tcgtcgcggg gatcctgcgc gagctcgccg tgcacgcggc gcacgtgctg
     3181 ttgcccgcgg tctttgtgcg cgcatcgaca cgcgggaccg gcgcggtgcc ccgggagatc
     3241 gtgggattct acgaaccgct cagcatgtcc tacgggctca atgatcccct gcggctggtc
     3301 cgctgcgccc tgcggatcct gaacatcgcc gagcgccgga tcgggccctc gccggagctc
     3361 gccaaggcgc tctccgggta cgcgtcggtg tgcatggccg ttccgctgtt cgcgcgggcg
     3421 cggcgctatc atcgtcgcgc gctggcgctc aacgaatcgc tgggtttcga gggcggcatc
     3481 gcgaaaagct gcgagctgat gggatacttc tacgagtgga gcggcgattt caccgccgca
     3541 cggctctcct tccagcgctc gctggacatc taccgccggc tgggcgacgc caaggaatac
     3601 gccatggtgc tcaacggcct ggagcactgc cattactatg cgggcgagta ctgggacgcg
     3661 ctcgaggtga accagcgcta ccggtcgagc gccgcgctgt ccgacgacgc ctatgccatg
     3721 agcgcggtgg acatttacta ctcgcagatc tatcgggagc agggcgacct cgaggcggcc
     3781 aaccggcatg ccgggcgcgc ccgcgacctc agccgagacc gggccatacc gtttaattac
     3841 tgctcgtcgc tcgtcgagct gggcatgaac gcgctggagg cgggcgagat cgacagcgcg
     3901 cgcacctgcc tcgaggaggc gcgcgcgctt cacgagcggg gaaactttct gcgccagtac
     3961 atcgttccgg tgtatcccgc gctggcgcag gcgcatctcg acgaattcct gcgcgacgag
     4021 ggcggtctca cgtccgaggc gcggaaacga cgcatggcgg gcctgctgcg aatctgccgc
     4081 ctggcggcga ggaagacgcg ccgctggcgg acccatcacg ggaccgcact gcgcatacag
     4141 gcgcaatgcc atgcaatcgc cgggcggcat gcggaggccg cggcgctctt tgcgcgcagc
     4201 attgccctgt gccgcgagtt ggggcggcgc ttcgagacgg cgcgcgggct ttacgagtgc
     4261 ggcatgttcc gcgcccatac cggcgacgac cgcgtctcgc ggccacttct cgaagaggcc
     4321 tatcggttgt tcacggagct cggggcgcag ctgcacgcat cgcgcatcgc cctcgttctg
     4381 ggcatccggg gcactgacga ccgcgagacc ccgctgaagc ggctcatggg acgggagcgc
     4441 gcgcaggcgg ccgggcggat cgggcgggag atcggcggcg ccgcatcgga agaggcgctt
     4501 ctggatctca tggcggcgcg cacgctggag atgaccggcg ctcggggatg cgtcatctat
     4561 cttggcgcgg acggcgacgg cgcccccgtg cggcggcacg agcgcggcga ggtgagcccg
     4621 gatgcgaacg cgcatcaacg cgccctcgcg gcgtcggtgt attcggccgg ggagtttctg
     4681 ctcgatgcag ggcccccggt cggcggatgc gcgctgctgt gcgcccccat ccggggggag
     4741 ggcggcgtga cgggcgcctg ctgcattgag gggccagcgg acgggatcgc cttcacggtt
     4801 gaggacggcg aatttctttc ggacatttac gcgacggcag ccatgcggct tcaggcgggg
     4861 ttcggcacgg cccgccccgc acgcacacga ggcgcagagc gcgacggagt cacgccgggc
     4921 accgaggaga agctgaatgc ggtgatcgcc tacatcggcc agaactatcg cgacgagctt
     4981 tcgcgcgagg ggctggccgc gatggtcggc atgagcccca atcacctcgg caagtatttc
     5041 gccatcgtca ccgggaggat ga
//
LOCUS       DAORHF010000261         5037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17390_length_5037_cov_4.832196, whole genome shotgun sequence.
ACCESSION   DAORHF010000261 DAORHF010000000
VERSION     DAORHF010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17390_length_5037_cov_4.832196"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="PKJ16_16160"
     CDS             <1..243
                     /locus_tag="PKJ16_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48579.1"
                     /translation="FDGIPALLGGLTNSEMRVRWAIYEELQFHMDGNDIVGAMRANAR
                     WSRYPDMVAKALRVQIENEMDALLTLNETKRKYGVR"
     gene            309..1112
                     /locus_tag="PKJ16_16165"
     CDS             309..1112
                     /locus_tag="PKJ16_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HNV48580.1"
                     /translation="MGVLLTALFPHYASSATRTAPGSVDPAAILRAYYIEHEDGGAAV
                     AQGNPDRRVSVASATKLVTALVARSMLRDDDLVTVPGAATRLIGARIGLTAGERYRVI
                     DLLHGMLMESGNDAAYSIAVRAAGSPARFAARMNEWCRANGFPKSSFADPAGLNRRSA
                     STARELVGIFRAFQSDPALARIITLKRHTIHSAGGRSIELNNSNYFIRYFRREFAGKT
                     GYTRAAGSCYVGWIKNGADRYYVAFLGARKGWQEQDVLFDALVARNGRR"
     gene            1099..2997
                     /locus_tag="PKJ16_16170"
     CDS             1099..2997
                     /locus_tag="PKJ16_16170"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV48581.1"
                     /translation="MAVGRGLRSAARWAIRSAPILAVASTALLAVVLAADAWIVSRAM
                     FAGPPRSVATELYARDGRLFHTLHHRRIVVDPVSAAALDPGRRATIESFLRDAAPRSG
                     VAGRLCALGGVRVALAWHAAALVSNSAPAFPNYALQRVRVYKNALLFAVAVPRMRILD
                     ALLNWKPVLGSLYGAQTVARTLHGAGLNELNDAALVEVLGYVYHRRSIPPERRHSSTR
                     ALWAADAASRPHEGVAGRYYVDACQRRAGGALDASAARVHTAMDLDLQTFLERLTEEY
                     FVSRERMPNNIRERMEPLPRLQAAAVVIDVATGEVRALVGGTSRDRRGWFNRAVRARR
                     QISSTFKPFLYAEAIERLGYTPATTIEDRPVRLTGRDGSPWRPRNFYSYYMGTVTIHK
                     ALAMSINTVSVRLIQRIGVDVMACRARSFFSMPGNDIGRRIRGEPSLALGAVDCTPLE
                     LARGYLVLARGGVDADPVLVREVVSTRGRSVVRNTEGRCPRRLLSAETAATVTDILSA
                     VFTPFGTAGAFMGKPLGFEMAGKSGSSPADSWFVGYNPEYLVCAWAGYDDPVAPPDRA
                     VPLFAVIPYWIMLMERCAPRETRFAVPQTLERRSFCADTGLPPDAECPVASGYFMSGR
                     SAPGSVSR"
     gene            2994..3800
                     /gene="mltG"
                     /locus_tag="PKJ16_16175"
     CDS             2994..3800
                     /gene="mltG"
                     /locus_tag="PKJ16_16175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HNV48582.1"
                     /translation="MTVRTIIRSLLAAYSLCCLVIAGYFSFHLVVPASFADSLVVPEG
                     STMRRIAERVSARTNISSRDFLSACLDMKYIRPLGIDAPSAEGYLYPDTYRLGPGVDA
                     RRLVRMMHRRFAEVVAALDARAPAGAALHELVVLASIVEKESPRDDEKPIIAGVFRNR
                     LGRGMKLQADPTVRYALNKPRGALTMDDIRHRSRYNTYIHHGLPPGPICSPGRAALAA
                     ALRPAQTPYLYFVSRGDGSHYFSRTLDEHRRAVDYFIRGRNNGFVDRRER"
     gene            3847..4413
                     /locus_tag="PKJ16_16180"
     CDS             3847..4413
                     /locus_tag="PKJ16_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48583.1"
                     /translation="MRFPQSIITSTRTTPMRMALLVAITLVGVAVACSSAGSDARAQA
                     VTAPPDVVPVNDKVLGFINARMGTTVGVGDCYALVSHALHYSGAEWDHYYHYGIPVSY
                     ETEEVRPGDIVQFLSVIVHSGPNDRYKNTFGYPMHTGVVYKIIDKLHYVIAHQNYKKQ
                     RFVLLTEVNFNDKRSGQYWIYRPFRRTN"
     gene            complement(4425..>5037)
                     /locus_tag="PKJ16_16185"
     CDS             complement(4425..>5037)
                     /locus_tag="PKJ16_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48584.1"
                     /translation="VLAVAPADVKYRDATLEEYKMKLASLRDDIAGGAGTAITETLEA
                     GRERFEAFRNIFRMILMSDNLQTLVATSIESPDALVGHLRDVRAGLPAQVQNLEDIAA
                     AFQGGRDAEGAEKLNGFIDFMYTYIRTCHQIPPIFRIDLEGIILDGTSLEDINADLTE
                     KLNMIVEVMENNDIISLTDILEYEIKPAFEGLGRYIDELLKAL"
BASE COUNT          765 a         1674 c         1650 g          948 t
ORIGIN      
        1 ttcgacggca ttccggcgct gctcggcggc ctcaccaaca gcgagatgcg ggtccggtgg
       61 gccatttacg aggaactgca gttccacatg gacggcaatg acatcgtcgg cgcgatgcgc
      121 gcgaacgcgc gctggtcccg ctatcccgac atggtcgcca aggcgctgcg cgtgcaaatc
      181 gaaaacgaga tggacgcgct gctcacgctc aacgagacca agagaaagta cggcgtccgg
      241 tagcgacagg cttcgtgcgc actgttcgaa aaccatccgc ccccgcattg cgcgggggtt
      301 tggttttttt gggggtcctg ctcacggcgc tttttccgca ctatgcctca tcggcgaccc
      361 ggaccgcacc cggttccgtc gatcccgccg ccatccttcg cgcctactac atcgagcatg
      421 aggacggcgg cgcggccgtc gcgcagggaa acccggatcg gcgcgtatcg gtcgccagcg
      481 ccaccaagct ggtcacggcg ctcgtcgccc gctcgatgct tcgcgacgac gatctcgtga
      541 ccgtgcccgg cgccgcgacc cggcttatcg gcgcgcgcat cggcctcacg gcgggcgagc
      601 gctatcgcgt tatcgacctc cttcacggca tgttgatgga gtcgggcaac gatgccgcgt
      661 actcaatcgc ggtgcgcgcg gccggctccc cggcgcggtt cgccgcgcga atgaacgaat
      721 ggtgccgggc gaacggcttc ccgaaaagct ccttcgccga tcccgccggc ctcaaccgac
      781 ggtccgcatc caccgcgcgc gagctggtgg gaattttccg cgccttccag tccgatccgg
      841 cgctcgcgcg gatcatcacc ttgaagcggc acacgataca cagcgccggc ggccgcagca
      901 tcgaactgaa caacagcaat tacttcatcc ggtatttccg gcgcgagttc gccgggaaga
      961 ccggctatac gcgcgccgcg ggcagctgct atgtcggatg gatcaagaac ggcgcggacc
     1021 gctactatgt cgccttcctg ggggcgcgga agggctggca ggagcaggac gttctgttcg
     1081 atgcgctcgt ggcgcgaaat ggccgtcggt aggggactgc gcagcgccgc gcgctgggcg
     1141 atccgctccg cgcccatcct ggcggtcgct tccaccgcgc tgcttgcggt cgttctcgcg
     1201 gcagacgcgt ggatcgtatc gcgcgcgatg ttcgcgggtc ccccgcgttc ggtcgcgacc
     1261 gaactgtatg cgcgcgacgg tcgcctgttt cacacccttc atcatcggcg gatcgtcgtc
     1321 gatccggtgt ccgcggcggc gcttgatccg gggcgtcgtg cgacgatcga gtcctttctg
     1381 cgcgatgccg ccccgcgctc cggagtcgcg gggcgtttgt gcgcgctcgg cggcgtccgc
     1441 gtcgccctgg cgtggcacgc ggcggcgctc gtatcaaaca gcgcgcccgc gtttcccaat
     1501 tacgcgctgc agcgcgttcg tgtttacaaa aacgcgctcc tgttcgccgt cgcggtcccg
     1561 cgcatgcgga tcctcgacgc gctcctcaac tggaaaccgg tgttgggatc gctctacggg
     1621 gcgcagaccg tcgcgcggac gctccacggg gcgggattga acgagctcaa cgacgcggcg
     1681 ctcgtcgagg tgctgggata tgtctatcat cgacggtcga ttccgcctga acgccgccac
     1741 agctctaccc gcgcgctgtg ggccgccgac gcggcgtcgc gtccacatga gggcgttgcg
     1801 gggcgctact atgtcgacgc gtgccaacgg cgtgcgggcg gcgcgctcga tgcgtccgcc
     1861 gcgcgcgtgc acaccgcgat ggacctcgac ctgcagacgt ttctcgagcg cctcaccgag
     1921 gaatatttcg tatcacgcga acggatgccc aacaacattc gcgagcgcat ggagcccctg
     1981 ccgcgcctgc aggcggcggc cgtggtcatc gatgttgcaa ccggcgaggt gcgggccctt
     2041 gtcgggggga cctcccggga ccggaggggc tggttcaacc gggccgtccg agcccggcgc
     2101 cagatatcgt ccacgttcaa gccgttcctc tatgccgagg cgatcgagcg gctcggctac
     2161 acgcccgcca ccacgatcga ggatcgcccg gtgcgcctca ccggccgcga tgggagtccc
     2221 tggcggccgc ggaattttta ctcctactac atgggcaccg tcacgatcca caaggcgctg
     2281 gccatgtcca tcaataccgt ctcggtgcgg ctcatccagc gcatcggcgt cgatgtgatg
     2341 gcgtgccgcg cgcgatcgtt tttttcgatg ccggggaacg atatcgggcg gcgcattcgg
     2401 ggcgagccct ccctcgcgct cggggcggtc gattgcacgc cgctggagct cgcgcgcggc
     2461 tacctggtcc tcgcgcgggg cggcgtcgac gccgatccgg tcctggtgcg ggaggtcgtt
     2521 tcgacacggg ggcgttccgt tgtgcgcaac accgaaggcc ggtgcccgcg gcgcctgttg
     2581 tccgccgaaa ccgccgcgac ggtcaccgac atcctctccg ccgttttcac gcccttcggg
     2641 accgcgggcg cttttatggg caagccgttg ggtttcgaga tggccgggaa aagcggatca
     2701 tcccccgcgg attcgtggtt tgtcggatac aacccggaat atctcgtgtg cgcctgggcg
     2761 gggtacgacg atccggtcgc accgcccgat cgcgcggtgc ccctgttcgc ggtcatcccc
     2821 tactggatta tgctgatgga gcgctgcgcg ccgcgtgaga cgcgattcgc ggtcccgcag
     2881 acgctcgagc gccgttcctt ttgcgccgac acgggtctcc cgcccgatgc cgaatgtccc
     2941 gtggcgagcg ggtatttcat gtccgggcgc tcggcgccgg ggagcgtgag ccggtgacgg
     3001 tacggacgat cattcggtct cttcttgcgg cctacagcct gtgctgcctc gtcattgcgg
     3061 gttatttctc gttccatctc gtcgttccgg cttcgttcgc cgattcgctt gtggtcccgg
     3121 agggctccac catgcggcgc atcgcggagc gcgtctcggc gcgcacgaat atatcgtccc
     3181 gcgacttcct ctcggcctgc ctggatatga agtacattcg cccgctgggg atcgacgcgc
     3241 cctcggccga agggtacctg tacccggaca cctatcgcct cgggcccggc gttgacgcgc
     3301 gccgtctcgt tcggatgatg caccgtcggt ttgcggaagt ggtcgccgcg ctcgatgccc
     3361 gggcgcccgc gggcgcggca ttgcacgagc tggtggtcct cgcatcgatc gtggagaagg
     3421 agtcgccccg ggacgatgaa aagccgatca tcgccggcgt gttccgcaac cgtctggggc
     3481 gcgggatgaa gctccaggcc gatcccaccg tgcgatacgc cttgaacaaa ccgcgcggcg
     3541 cgctcacgat ggacgacatc cggcatcgct cgcggtacaa tacctatatc caccacggcc
     3601 tcccgcccgg tcccatctgc tcgcccggtc gcgccgcatt ggccgccgcc ctgcgtccgg
     3661 cgcaaacgcc ctatctctat ttcgtgtcgc gcggcgacgg atcgcattat ttttcacgga
     3721 cgctcgatga gcaccggcgc gcggtggact attttatccg gggccggaac aacggatttg
     3781 tcgaccgcag ggaacgctga tttttttctt gaaatcaggg cgcatccgtg gcatggtgtc
     3841 ccggtaatgc gctttcccca gtccatcatc acctcgacgc gaacaacgcc gatgaggatg
     3901 gccctcctgg tcgccataac gcttgtcggt gtcgccgtcg cgtgcagctc cgccgggtcc
     3961 gatgcgcgcg cgcaggcggt aaccgcgccc ccggacgtgg tccccgtgaa cgacaaggtc
     4021 ctcggcttta tcaacgcccg gatgggaacg acggtcgggg tgggggactg ctatgcgctg
     4081 gtgagccacg cgctccatta cagcggcgcc gagtgggatc actactatca ttacggcatt
     4141 ccggtgagct acgagaccga ggaggtgcgg ccgggggaca tcgtccagtt cctttcggtg
     4201 attgtgcatt cggggccgaa cgaccggtac aagaacacct tcgggtatcc catgcacacg
     4261 ggcgtcgtgt acaagatcat cgacaagctg cactacgtca tcgcccatca gaattacaag
     4321 aaacagcgct tcgtgctgct caccgaggtc aacttcaacg acaagcggtc ggggcagtac
     4381 tggatctatc ggcccttccg gcggacgaac tagcgctctc ccgcttatag cgccttcaag
     4441 agctcatcga tgtagcgccc aagcccctcg aaggccggct tgatctcgta ctcgagaatg
     4501 tcggtgaggc tgatgatgtc gttgttctcc atcacctcca cgatcatgtt gagcttctcg
     4561 gtgagatcgg cgttgatgtc ctcgagcgag gtcccgtcga ggatgatccc ctcgaggtcg
     4621 atccggaaga tgggcgggat ttgatggcag gtgcggatgt aggtgtacat gaagtcgatg
     4681 aagccgttga gcttctcggc gccctcggcg tcccgcccgc cctggaaggc ggcggcgatg
     4741 tcctcgaggt tctggacctg ggcggggagg ccggcgcgga catcgcgcag gtgccccacc
     4801 agcgcgtcgg gcgattcgat gctggtcgcc acgagggtct ggaggttgtc gctcatcagg
     4861 atcatccgga agatattgcg aaaggcctcg aaacgctccc gtcccgcctc gagcgtctcg
     4921 gtgatggccg tcccggcgcc gccggcgata tcgtcgcgca gcgatgcgag cttcatcttg
     4981 tattcctcga gcgtggcgtc gcggtacttc acgtcggcgg gggccaccgc gaggacc
//
LOCUS       DAORHF010000262         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17476_length_5019_cov_4.992546, whole genome shotgun sequence.
ACCESSION   DAORHF010000262 DAORHF010000000
VERSION     DAORHF010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17476_length_5019_cov_4.992546"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..165
                     /locus_tag="PKJ16_16190"
     CDS             <1..165
                     /locus_tag="PKJ16_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HNV48585.1"
                     /translation="QFNVVDTDTLKDAMAHPENYPDLLVRVSGYCGYFTKLHRDLQLE
                     IIRRCEYGLD"
     gene            178..1104
                     /locus_tag="PKJ16_16195"
     CDS             178..1104
                     /locus_tag="PKJ16_16195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HNV48586.1"
                     /translation="MSAQPLILDIKGNSLDDGPGIRSVVFFKGCPLNCRWCQNPESKS
                     PKAELLWDAGKCTRCGTCIDLCHPAAIADSNTAFIDREKCTLCFACVDECPTGALARA
                     GRPMSVDEIVRQVVRYRDFFESSGGGVTLSGGEPTLWMEFIGELLRALKKEGIHTLLE
                     TCGQFSLDRFSALVLPWLDEIYFDIKLIDPLEHERHCGISNRVIIDNFVALNTLASGG
                     RITLLPRTPLIPGITDGEERIRALGEFYREQRVTRIGLLANNPIWLDKLKKLGRDDPF
                     GGADKIRGFYDGEGKKRILERFREYGITAVFG"
     gene            complement(1161..1589)
                     /locus_tag="PKJ16_16200"
     CDS             complement(1161..1589)
                     /locus_tag="PKJ16_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV48587.1"
                     /translation="MPHQVSVFAENKPGKIERITKVLSANGVNIRAITISDSGDYGII
                     KLLLDRPAEGCELLAKEGIAATLKDIVAVKMKDAPGGLHGIASILSANSINVEDAYGF
                     MVKSHEDAVFVFQVTSPQKAEKILKDEGLTILTDKELYFL"
     gene            complement(1599..2897)
                     /locus_tag="PKJ16_16205"
     CDS             complement(1599..2897)
                     /locus_tag="PKJ16_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015851903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV48588.1"
                     /translation="MYWNQDLETIDRPALERLQLSRLRAALERSVRAPFYRTFFETHR
                     VDIDTITTLEHLRDLPFTTKADLRTSYPDGMMAVPRRDVVRLHASSGTTGKSTVIFYT
                     QKDISEWADLIARSMVATGATPDDVFQNMMGYGLFTGGLGLHYGAERLGCLVIPSSSG
                     NSLKQLQLMQDFQATIMHITPSYALHLVEIIKENGVDPHDLTVKKAYLGAEPYTEATR
                     KKLEELWGMDAYNSYGLSEMNGPGVSFECVHKNGMHVWEDFYIIEIIDPDTGAVLPDG
                     AEGELVLTHVNREAMPMFRYRTRDLTRIIPEPCPCGRTHRRIERIKGRTDDMFIIGGV
                     NIFPSQIEAVLMAIPEVGNNYQIVLERDGSLDKVHVKVELYSKMFHGDMKELQTLRKK
                     ITEIVRSTIIVNPVIELMEPGSLPPSQGKAVRVIDNREQR"
     gene            complement(2919..3800)
                     /locus_tag="PKJ16_16210"
     CDS             complement(2919..3800)
                     /locus_tag="PKJ16_16210"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HNV48589.1"
                     /translation="MMHGRNVQFRIGGPLTPTVKALMIVNGAVFLLQTIAGWLSPGMI
                     EAYFGINHVGLIHQFMLWQPFTYMFLHGGFFHILFNLLALWMFGGDLEERWGKALFLR
                     YYIFSGIGAGLFIALMNYFVFITYNASPVTIGASGAIYAILLAYGLTWPDREVLLYFI
                     IPIKMKYLVLIFGGIEFFGTLASARGDGSGVSHIGHLGGLISGFIFIMWKLHGPRSAT
                     TSAQRSEGVIASFFKKERLKKKQKEIDTRIKAKAVIDRLLEKIAREGMGSLTPAERKE
                     LDWARKNYYPDDSATYH"
     gene            complement(3816..>5019)
                     /locus_tag="PKJ16_16215"
     CDS             complement(3816..>5019)
                     /locus_tag="PKJ16_16215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HNV48590.1"
                     /translation="HARLSGLPATRITEIVSMLNRGSETEEIFKTLSTTLKRFIPFNR
                     LTLALIDETTGSVVTKYIESDQQVLLGRGEEFPLERSSLNDAAERKVVIIINDLERYY
                     TEHASSVNAKRLLEEGMRSSLVLPLIINDRTRGFLIFASVTPHSFKEDHISFLESIVG
                     QISFSIQRGELLSELETHTEKLEQLVETRTREVLKTQKVTIFALSKLADARDVDTGQH
                     LERIRRFCALIAQVLKYSEHGKHITNQYLRDLYDSSILHDIGKVGIPDAILLKQGPLT
                     AEEFDIIKTHTTIGFNSLRSASKDLGEDTFLNMAMDITLYHHERWDGLGYPYGLKGDE
                     IPLSSRIVAISDVYDALTSKRPYKAPYEHEMAVEIMKDEQSHFDPLLFKIFLENNEEI
                     DRIRREFK"
BASE COUNT          874 a         1499 c         1655 g          991 t
ORIGIN      
        1 cagttcaacg tggtggacac cgacaccctg aaggacgcga tggcgcaccc ggagaactat
       61 ccggacctcc tggtgcgggt gtccggctac tgcggctact tcaccaagct ccaccgcgac
      121 ctccagctcg agatcatccg ccgctgcgag tacggcctcg actgacggga ccgtcggatg
      181 tccgcgcaac cgctgatact cgacatcaag ggcaattcgc tcgacgacgg gccgggaatc
      241 cggtccgtcg tcttcttcaa gggctgtccc ctgaattgcc gctggtgcca gaacccggag
      301 agcaagagtc caaaggccga actgttatgg gacgccggga agtgcaccag gtgcggcacc
      361 tgcattgatc tttgccatcc ggcagcgatt gctgattcaa atacagcttt catcgaccgc
      421 gaaaaatgca cgctgtgctt cgcgtgcgtc gacgaatgcc cgacgggcgc gcttgcccgc
      481 gcggggcggc ccatgagcgt cgacgagatc gtccggcagg tggtccgcta tcgggacttc
      541 tttgaatcgt cgggcggcgg cgtcacgctc tccggcggcg agcccaccct ctggatggag
      601 ttcatcggcg agctgctccg ggcgcttaag aaggagggga tccacaccct gctggaaacc
      661 tgcggccagt tttccctcga ccgattcagc gcgctggtcc tcccctggct ggacgagatc
      721 tacttcgaca tcaaactcat cgatccgctc gagcatgagc gccattgcgg gatttccaat
      781 cgggtgatca tcgacaactt cgtcgccctg aacacgctgg cgtcgggcgg ccgcatcacg
      841 ctccttcccc gcaccccgct catcccgggg atcaccgacg gcgaggagcg gatccgcgcg
      901 cttggcgagt tctaccggga acaacgcgtg acgcgtatcg gacttctcgc caacaacccc
      961 atctggctgg acaaactgaa gaagctcggg agggacgatc ccttcggcgg cgcggataaa
     1021 atcaggggct tctacgacgg cgaaggaaag aaacggatcc ttgagcgttt tcgggaatac
     1081 ggaatcaccg cggtgttcgg gtgatacgtc attctgctgc tcgatgatgt ccccgctccc
     1141 ttttcgagcg ggtgaggtca ctacagaaaa tacagctcct tgtccgtgag gatggtgagc
     1201 ccctcgtcct tgaggatctt ttcggccttc tgggggctcg tgacctggaa gacgaacacc
     1261 gcgtcctcgt ggctcttcac catgaaaccg taggcgtcct cgacattgat gctgttggcc
     1321 gagaggattg acgcgatgcc gtggaggccg ccgggcgcgt ccttcatctt cacggccacg
     1381 atgtccttga gcgtggcggc gatcccctcc ttcgccagga gctcgcagcc ctccgccggc
     1441 cggtccagca ggagcttgat gatcccgtaa tcgcccgagt cggagatggt gatggcgcgg
     1501 atgttgacgc cgttggccga gaggaccttc gtgatccgct cgatcttgcc cggcttgttc
     1561 tccgcgaaaa ccgatacctg atgcggcatg gcgccctcct agcgttgctc cctgttgtcg
     1621 atcacccgga cggccttgcc ctggctgggc ggcagcgatc cgggctccat gagctcgatg
     1681 accggattga cgatgatggt ggagcgcacg atctcggtga tcttcttgcg cagcgtctgc
     1741 agctccttca tgtctccgtg gaacatcttc gagtacagct cgaccttcac gtgcaccttg
     1801 tccagcgagc cgtcccgctc caggacgatc tggtagttgt tgcccacctc ggggatggcc
     1861 atgagcacgg cctcgatctg tgaaggaaag atgttcacgc cgccgatgat gaacatgtcg
     1921 tcggtgcgtc ccttgatgcg ctcgatgcgc cggtgcgtgc ggccgcaggg gcacggctcg
     1981 gggatgatgc gcgtgaggtc gcgcgtacgg tagcggaaca tgggcatggc ctcgcggttc
     2041 acatgggtga gcacgagctc gccctccgcg ccgtcgggga gcaccgcgcc cgtgtccgga
     2101 tcgatgatct cgatgatgta gaaatcctcc cagacgtgca tgccgttctt gtgcacgcac
     2161 tcgaaggaga cgccgggccc gttcatctcg gagaggccgt aggagttgta ggcgtccatc
     2221 ccccagagct cctcgagctt tttccgcgtg gcctcggtgt agggctcggc gcccagatag
     2281 gccttcttca cggtgaggtc gtgcgggtcc acgccgtttt ccttgatgat ctccaccagg
     2341 tgcagcgcat agctcggcgt gatgtgcatg atggtcgcct ggaaatcctg catgagctgg
     2401 agctgcttga ggctgttgcc ggacgaggag ggaatcacca ggcatcccag ccgctcggcg
     2461 ccgtagtgca ggcccaagcc gccggtgaac aggccgtagc ccatcatgtt ctggaacacg
     2521 tcgtccgggg tggcgccggt ggcgaccatg gagcgcgcga tgagatcggc ccactcgctg
     2581 atgtccttct gcgtgtagaa gatgaccgtc gattttcccg tcgtgccgct cgaggcgtgg
     2641 aggcgcacca cgtcgcgccg ggggacggcc atcatcccgt cgggatagct cgtacggagg
     2701 tcggccttcg tggtgaaggg cagatcgcgc aggtgctcga gcgtcgtgat cgtgtcgatg
     2761 tcgacgcggt gcgtctcgaa gaaggtccga tagaagggcg cgcgcacgga gcgctccagc
     2821 gcggcgcgga gccgggagag ctggaggcgc tccagcgccg ggcggtcgat tgtctcaagg
     2881 tcctggttcc agtacatggc agtggtccct ttcgcggtct agtgataggt ggcgctgtcg
     2941 tcgggatagt agttcttgcg cgcccagtcg agctccttgc gctcggcggg ggtgagggag
     3001 cccatgccct cgcgtgcgat cttctcgagc aggcggtcga tgacggcctt cgccttgatg
     3061 cgcgtgtcga tctccttctg cttcttcttc aggcgttcct tcttgaaaaa cgaggcgatg
     3121 acgccctcgg agcgctgcgc actcgtcgtc gcggatcggg gcccgtggag cttccacatg
     3181 atgaagatga atcccgagat gagcccgccc aggtggccga tgtggctcac gccgctgccg
     3241 tcgccccgcg cgctggcgag cgtcccgaag aactcgatcc cgccgaagat gagcaccaga
     3301 tacttcatct tgatggggat gatgaagtac aacagaacct cgcggtccgg ccaggtgagc
     3361 ccataggcga gcaggatcgc gtagatggcg cccgaggcgc cgatggtgac cggcgaggcg
     3421 ttgtaggtga tgaagacgaa gtagttcatg agcgcgatga agagcccggc cccgatgccg
     3481 gagaagatgt agtagcgcag gaagagcgcc ttcccccagc gctcctcgag gtcgccgccg
     3541 aacatccaca gcgcgagcag gttgaacagg atatggaaga agcccccgtg caggaacatg
     3601 taggtgaacg gctgccacag catgaactgg tggatgagcc ccacgtggtt gatgccgaag
     3661 tacgcctcga tcatgccggg cgagagccag ccggcgatcg tctgcagcag gaagacggcc
     3721 ccgttgacga tcatgagggc cttcacggtg ggcgtgagcg gcccgccgat tcggaactgg
     3781 acattacggc cgtgcatcat cacacctcgg aatcgtcatt tgaattccct gcggatccgg
     3841 tcgatctctt cgttattttc gagaaaaatc ttgaacagca ggggatcgaa gtgcgactgc
     3901 tcgtccttca tgatctcgac cgccatctcg tgctcgtagg gggccttgta ggggcgcttg
     3961 ctggtgagcg cgtcgtacac gtcgctgatc gcgacgatcc gcgacgagag cgggatctcg
     4021 tcgcccttga gcccgtaggg gtaccccagg ccgtcccagc gctcgtggtg gtacagggtg
     4081 atgtccatgg ccatattgag aaaggtgtcc tcgcccaggt ccttggaggc cgagcgcagc
     4141 gagttgaacc cgatggtggt gtgggtcttg atgatgtcga actcctcggc cgtgagcggc
     4201 ccctgcttca acaggatcgc gtcggggatc cccaccttgc cgatgtcgtg cagaatggac
     4261 gagtcgtaga ggtcgcggag atactggttg gtgatgtgct tcccgtgctc ggagtacttg
     4321 agcacctgcg cgatgagggc gcagaagcgg cggatgcgct cgaggtgctg gccggtgtcc
     4381 acgtcgcgcg cgtcggcgag tttgctgagc gcgaagatgg tcaccttctg cgtcttgagg
     4441 acctcgcggg tgcgcgtctc cacgagctgc tccagcttct cggtgtgcgt ctcgagctcg
     4501 gacaggagct cgccgcgctg gatgctgaag gagatctgcc ccacgatgga ttccaggaag
     4561 gagatgtggt cctccttgaa cgagtgcgga gtgaccgagg cgaagatgag aaagccccgc
     4621 gtgcggtcgt tgatgatcag cggcagcacc agcgacgagc gcatgccctc ctcgaggagc
     4681 cgctttgcgt tcacggaact ggcgtgctcg gtataatagc gctcaagatc gttgatgatg
     4741 atgacgacct tccgctcggc ggcgtcattg agcgaggagc gctcaagggg gaactcctcg
     4801 ccccgcccga gcaggacctg ctggtccgac tcgatgtact tggtgaccac cgagccggtc
     4861 gtctcgtcga tgagggcgag cgtgagccgg ttgaacggga tgaagcgctt gagcgtggtg
     4921 cttaaggtct tgaatatctc ctcggtctcg ctgccgcggt tgagcatgga gacgatctcg
     4981 gtgatgcgcg tcgccggtaa gcccgacagc cgtgcatgc
//
LOCUS       DAORHF010000263         4995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17597_length_4995_cov_5.403846, whole genome shotgun sequence.
ACCESSION   DAORHF010000263 DAORHF010000000
VERSION     DAORHF010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17597_length_4995_cov_5.403846"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PKJ16_16220"
     CDS             complement(<1..789)
                     /locus_tag="PKJ16_16220"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HNV48591.1"
                     /translation="MSPTNNRFLREEYAARINRVMDYIDANIDAELSLDALARVARFS
                     PYHFHRIFRALIGEPLFQFIQRIRLEKAAAQLIANPKKPITRIALDCGFSGSAAFARA
                     FREAFGMSASAWRASGGDLRNPGKPNSKQGKTPRNEGKDSRASSVYDTPITTTRRKCM
                     NKRVSTNVEVKELPETTVVYVRNVGPFKGNSALFGSLIGKLCAWAGPRGHLGPDTRIM
                     AVYHDNPDITKEQKLRLSVCLPVSPNASVDGEIGMMTIAGGRYAV"
     gene            941..3178
                     /locus_tag="PKJ16_16225"
     CDS             941..3178
                     /locus_tag="PKJ16_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HNV48592.1"
                     /translation="MELIILSLVILGILAIFDLVVGVSNDAVNFLNSAIGARVASIKV
                     ILIVAGLGIMAGVTFSSGMMEVARKGIFHPEHFILPELLIIFVAVMLTDVLLLDLFNT
                     FGLPTSTTVSIIFELLGASVAVSLIKIYAANESFVLLGQYINTAKVLAIISGIFLSVA
                     IAFSVGIAAQFFSRMLFTFDYGKRLRRYGGIWGGLALTMIVYFIIIKGAKGASFLTQQ
                     HMDWIHVNTVAILCATFVGSTALLQALIVVANVNILRPIVLAGTFALAMAFAANDLVN
                     FIGVPLAGFGAYASGMAAANPLTATMEALKEPVRTDTLLLLAAGAVMVLTLFYSKKAR
                     TVTKTEVSLGRQDEGAERFESFFLARIIVRMGTGFMEGLRTVLPGRLVRAVAARKDMS
                     MAQPAATADGEVPSFDLIRASVNMMVASALVSFATSLKLPLSTTYVTFMVAMGTSLAD
                     GAWDRESAVYRVSGVLMVIGGWFFTAFIAFSVAFGYAFFLYHFRIPALIVLIGLSAFV
                     LWKNHKKHREIVETEREGEVYNLKKNKDPMLAVSTSFEQTGRLLEQVSASLGRSFTGL
                     TSYDRVELRIAKNDAKKIQDWTNIIIANIFKALRLLHQSEMKTAQRYTQIISNLQEIA
                     ESQRDVAMRSYLHVLNHHKGLLAVQVKELDAVVRGVRDLLDVTAASLRQDRIVDMPLL
                     REKSDALASRISEFDANQITRVQNESSKTRLSILFYGYLVSASKISACTKNLLEIFHR
                     SLKLK"
     gene            3222..4190
                     /locus_tag="PKJ16_16230"
     CDS             3222..4190
                     /locus_tag="PKJ16_16230"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HNV48593.1"
                     /translation="MKTVKTTPATPEAITHAAAIIRSGGLVAFPTETVYGLGANALDP
                     IAVARIFEAKNRPFFDPLIVHVANVGALDELVMGVDARAVALAAAFWPGPLTIVFPKQ
                     AVVPDIVTAGLSTVAIRLPAHPVARALIDEAHVPIAAPSANPFNYISPTRAAHVAAQL
                     GGRVDMILDGGDCMVGVESTIVECAGPGTRILRHGGLPLEEIERVIGPLRDATVHRDA
                     PIAPGQLPAHYSPRTRLAIVRDAHHPPAVSDAALLAFRTPPRSLRYAAVEVLSPSGDL
                     REAAANLFSALHRLDEAGVAIIYAEAVPEEGLGRAIMDRLRRAAAK"
     gene            4249..>4995
                     /locus_tag="PKJ16_16235"
     CDS             4249..>4995
                     /locus_tag="PKJ16_16235"
                     /inference="COORDINATES: protein motif:HMM:NF018917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HNV48594.1"
                     /translation="MSLFVKGIVSPFRALRIVREHPGLKRFFVVPFFINMLIIVGVCY
                     VSAAHLFPWVQGLLPQGDAWYWTALRYVVAPLFFVLLGLAIMLFYSILGNIIMAPFND
                     PLSAKVERLLGGDSFDEPITVRGVVADMLRIAKSVAAMLALYLVYIIAIMFLNIIPLI
                     GSPLYSLLSILGMGFFLGFQFFDFPLDRRKLGFRKKLGVVWGHKAMTIGLGLGFFALS
                     LVPVFGFLGLNLGTVAATALFVEHIRPEMNA"
BASE COUNT          798 a         1538 c         1604 g         1055 t
ORIGIN      
        1 gaccgcatag cgcccgccgg cgatcgtcat catgccgatc tcgccatcga cactcgcatt
       61 cggtgagacg ggcaggcata ccgagagacg gagcttctgt tccttcgtga tgtcggggtt
      121 gtcgtgatag accgccatga tcctcgtgtc gggtcccaga tgcccgcgcg gtcccgccca
      181 ggcgcagagc ttcccgatga gcgatccgaa gagcgcgctg tttcccttga aggggcccac
      241 attgcgcacg tagacgaccg tggtttccgg cagctccttc acttccacgt tcgttgatac
      301 ccgcttgttc atgcatttcc tccgtgttgt tgtgataggc gtatcataca cggacgacgc
      361 gcgggaatct ttcccctcgt tgcggggggt tttcccctgc ttgctattcg gttttcccgg
      421 attgcgcaga tcgccgccgg aggcgcgcca ggcgctcgcg ctcatcccga aggcctcccg
      481 gaaggcgcgc gcgaacgcgg ccgacccgga gaagccgcaa tcgagcgcga tccgggtgat
      541 cggtttcttg gggttcgcga tcagctgcgc ggcggccttc tcgaggcgaa tgcgctggat
      601 gaactggaag agcggctcgc cgatcaacgc gcggaagatg cggtgaaagt ggtacggcga
      661 gaagcgcgcc acgcgcgcca atgcgtcgag cgagagctcc gcgtcgatgt tcgcgtcgat
      721 gtagtccatc acgcggttga tgcgcgcggc atactcctcg cggaggaagc ggttgttggt
      781 cggcgacatg gggccgatcc tgcccggcaa tcgcgcgcct gtcaagcgca aggcggcgac
      841 gggaaagtgg ggaacggccc tcatcgtgct tgacatccgg cgccgggtgg cgcagcgtgc
      901 tcccgcgtga gcgcgtgcgc ggcaaggacg gtacccggct atggaactga tcattctctc
      961 gcttgttatt ctgggaatcc ttgcgatatt cgacctcgtc gtgggcgtca gcaatgacgc
     1021 ggtgaacttc ctcaattccg cgatcggcgc gagggtcgcc agcatcaagg tcattctgat
     1081 cgtggccggg ctgggcatca tggcgggcgt gaccttctcc agcggcatga tggaggtcgc
     1141 gcgcaagggc atcttccacc ccgagcattt cattttaccc gagttgctca tcatcttcgt
     1201 cgcggtcatg ctcaccgacg tgctgctgct cgatctcttc aataccttcg gcctccccac
     1261 ctcgaccacg gtgtcgatca tcttcgagct cctgggcgcc tccgtcgcgg tctccctgat
     1321 caagatctac gcggcgaacg agagttttgt cctgctgggg cagtacatca acaccgccaa
     1381 ggtgctggcg atcatttcgg ggattttcct ctctgtcgcg atcgccttta gcgtcgggat
     1441 tgcggcgcag tttttttcgc gcatgctgtt caccttcgat tacgggaagc ggctcaggcg
     1501 gtatggcggg atctggggcg gtctggccct caccatgatc gtgtacttca ttatcatcaa
     1561 gggcgcgaag ggggcgtcgt ttctcacaca gcagcacatg gactggatac acgtcaacac
     1621 cgtggcgatc ctgtgcgcga ccttcgtcgg gtcgaccgcg cttctgcagg cgctgatcgt
     1681 cgtcgccaac gtgaatatcc tgcgcccgat cgtcctcgcg gggaccttcg cgctcgcgat
     1741 ggcctttgcg gcgaacgacc tcgtcaattt tatcggcgtg cccctcgcgg gatttggcgc
     1801 ctacgcgtcg ggaatggccg cggcgaatcc gctcacggcg actatggagg cgctgaagga
     1861 gccggtacgg accgacaccc tgctgttgct cgccgccggc gcggtgatgg tgctcacgct
     1921 cttctattcg aagaaggcgc gaacggtgac caagaccgag gtgagccttg gccgccagga
     1981 cgagggcgcg gagcggttcg aatcgttctt tctcgcgcgg atcattgtgc ggatggggac
     2041 cggcttcatg gaggggctgc ggacggtgtt gcccggacgg ttggtgcgcg ccgtcgcggc
     2101 ccgcaaggac atgtcgatgg cgcaaccggc ggcgaccgcc gacggggagg tcccctcgtt
     2161 cgacctgatc cgcgcctcgg tgaacatgat ggtggccagc gccctggtgt cgttcgcaac
     2221 ctcgctgaag ctgccgctct cgaccacgta cgtgaccttc atggtggcca tggggacctc
     2281 gctcgccgac ggcgcctggg accgggagag cgccgtctat cgcgtgtccg gcgtgctcat
     2341 ggtcatcgga gggtggtttt tcaccgcgtt tatcgccttt agcgtcgcgt tcggctatgc
     2401 gttttttctc taccatttca ggatccccgc cctcatcgtc ctcatcgggc tctccgcgtt
     2461 cgtactgtgg aagaaccaca agaagcaccg ggagatcgtc gaaacggagc gcgagggcga
     2521 ggtgtacaac ctgaagaaga acaaggaccc gatgctcgcc gtgtccacgt ccttcgagca
     2581 gaccgggcgg ctcctcgagc aggtgtcggc gtcgctcggg cgcagcttca ccggactcac
     2641 ctcgtacgac cgcgttgagc tgcgcatcgc gaagaacgat gcgaaaaaaa tccaggactg
     2701 gacgaacatc atcatcgcca atattttcaa ggccctgcgg ctccttcatc aatccgaaat
     2761 gaagaccgcc cagcgctata cgcaaatcat cagcaacctt caggagatcg ccgagagcca
     2821 gcgcgacgtg gccatgcgtt cgtatctgca tgtcctgaac caccacaagg gcctgctggc
     2881 ggtgcaggtc aaggagctcg atgcggtggt gcgcggcgtt cgcgatctcc tcgatgtgac
     2941 cgccgcctca ttgaggcagg accggattgt agacatgccc ctcctgcgcg agaagagcga
     3001 cgcgcttgcg agccgtattt cggaattcga cgccaaccag attacccggg tgcagaacga
     3061 aagctcgaag acgcggctga gcatcctctt ctatggatac ctcgtgagcg cctcgaagat
     3121 ctccgcctgc acgaaaaacc tgctcgagat cttccaccgc tcgctcaagc tgaaataacc
     3181 gcacccgcaa cgggtgtgtt attgtaacga agcctggacc tgtgaaaacc gtgaagacga
     3241 ctcccgccac ccccgaagcc atcacgcacg ccgccgcgat catccgctcc ggggggctgg
     3301 tcgcgtttcc caccgagacc gtctatgggc tcggggcgaa tgcgcttgat ccgatcgccg
     3361 tcgcgcggat cttcgaggcg aagaaccgcc cgttcttcga tccgctcatc gtgcatgtgg
     3421 cgaatgttgg cgcgctggat gagctcgtga tgggggttga tgcgcgtgcg gttgcgcttg
     3481 ctgcggcgtt ttggccgggg ccgcttacga tcgtattccc caagcaggcg gtcgttccgg
     3541 acattgtgac cgcggggctt tcaacggtgg cgatccgcct tccggcgcac cccgtcgcgc
     3601 gggcgctcat tgacgaagcc cacgtcccga tcgccgcgcc gagcgccaat cccttcaatt
     3661 acatcagtcc cacgcgggcc gcgcatgtgg cggcgcagct cggggggcgc gtggacatga
     3721 tcctggacgg gggcgactgc atggtgggcg tcgaatcgac catcgtggag tgcgcggggc
     3781 cggggacccg gatcctgcgg catggcggcc tgccgctcga ggagatcgag cgggtgatcg
     3841 gtccccttcg cgatgcgacg gtccatcgtg acgccccgat cgcgccgggg cagctccccg
     3901 cgcactattc gccgcggacg aggctcgcga tcgtacgcga tgcccatcat ccccccgccg
     3961 ttagcgatgc ggcgctcctt gcgtttcgga ccccgccgag gagcctgcgc tatgccgccg
     4021 tcgaggtgct ctcgccgtcg ggggacctgc gtgaggccgc cgcgaacctc ttctccgcgc
     4081 tccaccggct cgacgaggcc ggcgtcgcca tcatttatgc cgaggccgtt cccgaggagg
     4141 gattgggccg ggcgatcatg gaccgcctgc ggcgcgcggc cgcgaaataa ttgtatttga
     4201 cattttcgtt ttgcgcgtag agcgtctggc acgggagagg aggacgccat gtcgctgttc
     4261 gtcaagggaa ttgtgagccc gtttcgggct ttgcgcatcg tgcgcgagca tccgggttta
     4321 aagcggttct tcgttgtacc gtttttcatc aacatgctga tcatcgtcgg ggtgtgctat
     4381 gtctcggccg cccatctctt tccgtgggtg caggggctgt tgccgcaggg tgacgcctgg
     4441 tactggacgg cgctgcgcta tgtggtcgcg ccattgttct ttgtgctcct ggggttggcg
     4501 atcatgctct tttattccat cctgggaaat atcatcatgg cgccgtttaa tgatccgctc
     4561 tcggcgaagg tcgagcggct gcttggcggc gattcgttcg acgagccgat cacggttcgc
     4621 ggggttgtcg cggacatgct ccggatcgcc aagagcgttg ccgccatgct ggcgctctat
     4681 ctcgtctata ttatcgccat catgttcctg aatatcatcc ccctcatcgg aagtccgctc
     4741 tattcgctgt tgagcatcct ggggatgggg tttttcctcg gcttccagtt cttcgatttt
     4801 ccgctggacc ggcgtaagct ggggtttcgg aaaaagctgg gcgtggtgtg ggggcacaag
     4861 gccatgacca tcggcctggg tctgggattt tttgcgctct cgctggtgcc ggtgttcggc
     4921 ttcctggggc tcaacctggg gaccgttgcg gcgacggcgc tgttcgtgga gcacattcgg
     4981 ccggagatga atgcg
//
LOCUS       DAORHF010000264         4962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17759_length_4962_cov_5.235786, whole genome shotgun sequence.
ACCESSION   DAORHF010000264 DAORHF010000000
VERSION     DAORHF010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4962
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17759_length_4962_cov_5.235786"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PKJ16_16240"
     CDS             complement(<1..367)
                     /locus_tag="PKJ16_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HNV48595.1"
                     /translation="MTTQHRHKRKDGLRRQAQIMSLAMRLFAEKGYHSTSVDDIITAA
                     QIVKGTFYLHFKSKQDLLNSIVDTHLNLLYESLKILDISLPRPLDEIRQLYSDVGAQL
                     MQDPELKVFVKLMLREVMGH"
     gene            complement(364..750)
                     /locus_tag="PKJ16_16245"
     CDS             complement(364..750)
                     /locus_tag="PKJ16_16245"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48596.1"
                     /translation="MRNYDDIESEDCYDMAVEWLHARDYEKAIDCLKRSIALNPNFVY
                     AYVTLAQAQARSGRLSSAVDTLLHALRVDPAFDRLHWLIAKYAHKRGDIPTALAHVNA
                     AIDMTGSPLYLRGREIILNARKRGRP"
     gene            complement(747..1208)
                     /locus_tag="PKJ16_16250"
     CDS             complement(747..1208)
                     /locus_tag="PKJ16_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48597.1"
                     /translation="MAELTRVTRHTGTSAGAVESAHPIDRGKTYELYFRMDQTDECLK
                     VGLTAKTVAAGLGGAHRIVISYFAVDKLVDISAFQSVAPGKSVYSEIGRNFDHRWNRE
                     RELLVCSSREDPIKKFTPGLYKIRFTTFSPAPFSFEVTIHSRQAIRFFDRP"
     gene            1408..2418
                     /locus_tag="PKJ16_16255"
     CDS             1408..2418
                     /locus_tag="PKJ16_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HNV48598.1"
                     /translation="MKILLLDDEPERIDRVSERLAGGGFEVVVVDGPQALEPAIVEHR
                     PSLVVFGEGRFREYGALGAGRTQSATHHPVDTGKRFDAAEMARAIERGEFALVYQPIV
                     SLETGGVSGFEALIRWHHPDRGLVGPDNFIPLAEESGLIIALGFFAVAEAARQIAAWR
                     AIIHAERPLSVSVNLSTVQFIHPDLVEVLRETVLPYGLPPGTIRFEITESALMQDMEQ
                     ANIALLKLKANGFPLYMDDFGTGYSSLSYLRHFPVDVLKIDKSFVRWMGVDEESDEIV
                     RIIIHLAHTLRMKIVAEGVETPETLNKLRELGADYGQGYLFGKPLAPADAEALLRADP
                     RW"
     gene            2471..4198
                     /locus_tag="PKJ16_16260"
     CDS             2471..4198
                     /locus_tag="PKJ16_16260"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HNV48599.1"
                     /translation="MKLTRYLVPTLKENPSDAVVVSHRLMIRAGLIRKETAGMYAYLP
                     LGLRALQKITAIIREEMERAGALEFLMPELTGAELWKESGRWDTMGKEMFRITDRNGM
                     AYALAPTHEEAFTAMVRSLISSYRDLPLNVYQINTKFRDEIRPRFGVMRSKEFIMKDA
                     YSFDLDEAGLETSYQAMRQAYRRIFQRAGIDTIPVEADTGAMGGSASEEFMVASEVGE
                     EVLLICPQCGYRANQERAAHARKASAGGGETPAACAEVSTPDVKTIDDLVAFFGCTAD
                     RFLKSLVYLADGKPVMAVLTGDRDVNEVKLRNLLGAVELELAPDSVVEEVTKAPVGFA
                     GPVNALAVRTVFDIAVRGVVNGITGANKKDVHFNGVNPGRDFTIKEEGDIVLAVAGDA
                     CPACGTPLGEKKGIEVGHIFKLGYKYTKSMNVSVLDEKGAAITPIMGCYGIGVNRTMA
                     AIIEQHHDDKGIIWPVSVAPFAVHLVGLGKSDDELARVEEIYALLCAAGIEVLYDDRH
                     ATPGFKFADADLIGMPIRVTVGKGYFSGGEIEVKRRAGGDTVKVGRDGLMSAVRAIID
                     EERARLNAS"
     gene            4195..>4962
                     /locus_tag="PKJ16_16265"
     CDS             4195..>4962
                     /locus_tag="PKJ16_16265"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNV48600.1"
                     /translation="MSDLLNYVSRDAFGLAYVLLLSFLIGVVRESSYQNHAIKFGGVR
                     TYPLIGLFGYIAYLIDKERLSFFIVGALIIGAIIALFYYYHIKKDRSGFISEITALIT
                     YLIGGLAARGEIWAPLAITILMILIVQSRTYLENFARTIPPEEVTTFVKFMLLSAVIL
                     PVVPNQDFTEVGLNPFKIWLFVVAVNGLSYVSYLLQIFFKGRGGVFLFSVLGGAYSST
                     ITTIVLAKRARELAADATGAIARHYAGIVLASSMMYLR"
BASE COUNT          887 a         1492 c         1620 g          963 t
ORIGIN      
        1 cgtggcccat cacctcgcgc agcatgagct tgacgaacac cttgagctcg gggtcctgca
       61 tgagctgcgc gccgacatcg ctgtagagct ggcggatctc gtcgaggggg cgcgggagcg
      121 agatgtcgag gatcttgagc gattcgtaca gcaggttgag gtgcgtgtcc acgatgctgt
      181 ttagaaggtc ctgtttgctc ttgaagtgca ggtagaaggt ccccttgacg atctgggcgg
      241 ccgtgatgat gtcgtccacc gacgtggagt gatagccctt ctcggcaaag agccgcatcg
      301 ccaggctcat gatctgcgcc tgtcggcgaa ggccgtcctt gcgtttgtgc cggtgctgcg
      361 tggtcatggc cgccctctct tccgtgcgtt caagataatc tccctgcccc gaagatacag
      421 gggcgatccc gtcatgtcga tggccgcatt cacatgcgcc agcgcggtgg ggatgtcgcc
      481 gcgcttatgc gcgtacttgg cgatgagcca gtgcagacgg tcgaaggcgg gatcgacacg
      541 aagcgcatgc agcagcgtgt ccaccgccga cgacagccgt ccggagcgcg cctgcgcctg
      601 ggcgagggtc acataggcgt agacgaaatt cgggttgagc gcgatggagc gcttgaggca
      661 gtcgatcgcc ttctcgtaat cgcgcgcgtg gagccactcg acggccatgt catagcagtc
      721 ttcggactcg atgtcgtcgt agttcctcat ggacggtcga aaaagcgtat cgcctgtctc
      781 gagtgaatgg tcacctcaaa gctaaatggc gcggggctga atgttgtaaa gcgaatttta
      841 tacagcccgg gtgtaaattt tttgatcggg tcttcccggc tcgagcatac gagtagctcg
      901 cgttcccggt tccagcggtg gtcgaagttg cgcccgatct ccgagtacac gctcttcccc
      961 ggcgccacgc tctggaaggc gctgatgtcg accagcttgt ccaccgcgaa atagctgatg
     1021 acgatccggt gcgcgccgcc aagacctgcg gcaacggttt tcgcggtgag cccgaccttg
     1081 aggcattcgt cggtctggtc catccggaag tataactcgt atgtctttcc ccggtcgatg
     1141 ggatgtgcgc tctcgacggc gccagcggag gtcccggtat gccgggtcac gcgcgtgagc
     1201 tcggccatgg tatcggccga accgcactga agaaaaaaaa ttgaggccag gcaaagtgct
     1261 tgatatgctg tgtgcgcgac ccgtatgctt gtcgcgatcc atccgttcgt gtgttgtacc
     1321 atgcgcatcc cggtctctcc cgttgggcct tccgggtatg cacgatgact cagcgcacgg
     1381 taaaatgcaa tgaaaaaccg gccgcccatg aaaatactgc ttcttgacga cgaaccggag
     1441 cgcatcgacc gcgtgtccga acgccttgcg ggcggcggct tcgaggtcgt tgttgtggat
     1501 ggtccgcagg cactcgagcc ggccatcgtc gagcaccgcc cgtcgcttgt cgtctttggc
     1561 gaggggcggt ttcgcgagta cggtgcgctc ggcgccggcc gcacgcaatc cgcgacccat
     1621 cacccggtcg atacgggcaa gcgcttcgac gcggcggaga tggcccgcgc aatcgagcgc
     1681 ggggaattcg cgctcgtgta tcaacccatc gtgtcgctcg aaacgggcgg ggtgagcggt
     1741 ttcgaggcgc tcattcgctg gcatcatccc gatcgggggc tggtgggtcc cgacaacttc
     1801 attccgcttg ccgaggagag cggtctcatc atcgcgctcg ggtttttcgc ggttgccgag
     1861 gcggcgcgcc agatcgccgc gtggcgcgcg atcatccacg cggaacggcc gctgtcggtg
     1921 agcgtgaacc tgtccaccgt gcagtttatc catcccgatc tggtggaggt gcttcgggag
     1981 accgttcttc cctacggcct gccgccgggg acgatccgat tcgagatcac cgaatcggcg
     2041 ctcatgcagg acatggagca ggcgaatatc gccctgctca agctgaaggc gaacgggttt
     2101 cctctctaca tggacgattt tggtaccggg tactccagcc tgagctacct gcgccatttt
     2161 ccggttgacg tgcttaaaat cgacaagtcc tttgtccggt ggatgggcgt cgacgaggag
     2221 agcgacgaga tcgtgcgcat catcatccac ctcgcgcata ccctgaggat gaagatcgtc
     2281 gccgagggcg tcgaaacgcc cgagacgctg aacaagcttc gagagctggg cgccgactat
     2341 gggcagggct acctgttcgg gaagcctctg gcgccggcgg acgccgaagc attgcttcgc
     2401 gccgatcccc ggtggtagcg cgcgccgacg ccgatcatcc gactatatca ctgatggagt
     2461 ctgctgccgt atgaaactga cccgctatct cgttcccacg ctcaaggaaa atccctccga
     2521 cgctgtcgtg gtaagccacc ggctgatgat ccgcgccggg ctcatccgca aggagaccgc
     2581 cgggatgtac gcctatctgc cgctgggcct tcgcgccctg cagaagatca ccgcgatcat
     2641 ccgcgaggag atggagcgcg ccggggcgct ggagtttctc atgcccgagc tcaccggcgc
     2701 ggagctctgg aaggagtccg gccgatggga caccatgggc aaggagatgt tccgcattac
     2761 ggaccgcaac gggatggcgt atgcgctcgc gcccacgcac gaggaggcct tcaccgccat
     2821 ggtccgctcg ctcatctcgt cgtaccgaga cctgccgctg aacgtctacc agatcaacac
     2881 caagttccgc gacgagatcc gcccgcgctt cggggtgatg cgcagcaagg aattcatcat
     2941 gaaggacgcc tattcctttg acctggacga ggccggcctc gagacatcgt atcaggcgat
     3001 gcggcaggcg tatcgccgca tattccagcg cgcggggatc gacaccattc cggtcgaggc
     3061 cgatacgggg gcgatgggcg ggagcgcctc cgaggagttc atggtggcct cggaggtggg
     3121 cgaggaggtg ctgctcatct gcccacaatg cggataccgg gcgaaccagg agcgcgccgc
     3181 ccatgcccgg aaggcgtccg caggcggcgg tgagacgccg gccgcgtgtg cggaggtctc
     3241 cacgccggac gtgaagacca tcgacgacct cgtcgccttt ttcggatgca cggccgatcg
     3301 attcctgaag agcctggtct atctggccga cgggaagccg gtgatggccg tgctaacggg
     3361 cgatcgcgac gtcaacgagg tgaagctcag gaaccttctc ggcgccgtgg agctcgagct
     3421 tgctccggac tcggtggtgg aggaggtgac caaggcgccg gtcggcttcg ccggtccggt
     3481 gaacgcgctc gcggtccgga cggtgttcga catcgccgtg cgcggcgtcg tgaacggaat
     3541 caccggggcg aacaaaaagg acgtccattt caacggcgtc aaccccggcc gcgactttac
     3601 cattaaagaa gagggtgata ttgtcctggc ggtggcgggg gacgcctgcc ccgcatgcgg
     3661 aacgcccctc ggcgagaaga aggggatcga ggtgggacac atcttcaagc tggggtataa
     3721 atacacgaag tcgatgaacg tgagcgtgct cgacgagaag ggcgcggcga tcacgcccat
     3781 catgggctgc tacggcatcg gcgtgaaccg gacgatggcg gcgatcatcg agcaacacca
     3841 tgacgacaag ggcatcatct ggcccgtgtc ggtcgcgcca ttcgcggtgc acctcgtggg
     3901 gctgggaaaa agcgatgatg aactcgcgcg cgtcgaggag atctatgcgc tgctgtgcgc
     3961 cgccgggatc gaggtgctct atgacgatcg gcacgcgacc cccggcttca agttcgccga
     4021 cgcggacctt atcggcatgc ccatacgggt gacggttggc aaggggtatt tctcgggcgg
     4081 cgagatcgag gtgaagcgcc gcgcgggcgg cgatacggtc aaggtcggtc gtgacgggct
     4141 gatgtcggcg gtgcgcgcca tcatcgacga ggagcgtgcg cgcctgaacg cctcatgagc
     4201 gatctgctga actacgtctc ccgcgacgcc ttcgggctcg cctacgtgct tctgctcagc
     4261 ttcctgatcg gcgtcgttcg cgagagcagc tatcagaatc acgcgatcaa gttcggcggg
     4321 gtgcggacct atccgctgat cggcctgttc ggctatatcg cctacctgat cgacaaggag
     4381 cgactctcat ttttcatcgt aggcgccctg atcatcggcg ccatcatcgc gctgttttat
     4441 tattatcaca tcaaaaagga ccgctccggc ttcatctcgg agatcacggc gctcatcacc
     4501 tacctgatcg gcgggctggc cgcccgcggc gagatctggg cgccgctggc gatcaccatc
     4561 ctcatgatcc tcatcgtgca gagccggacc tacctggaga acttcgcgcg gacgatcccg
     4621 ccggaggagg tgacgacctt cgtgaagttc atgctcctct cggcggtgat cctgccggtg
     4681 gtgcccaatc aggacttcac cgaggtgggg ctcaatccgt tcaagatatg gctcttcgtg
     4741 gtcgcggtga acgggctttc gtacgtgagc tacctgctgc agatattctt caaggggcgc
     4801 ggcggggtat ttctcttttc cgtactcgga ggcgcctact ccagcaccat taccacgatt
     4861 gtcctggcga agcgcgcgcg agagctcgcc gccgacgcga caggggccat cgcgcgccac
     4921 tacgcgggca tcgtcctggc gtcgtcgatg atgtatctgc gg
//
LOCUS       DAORHF010000265         4960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17768_length_4960_cov_5.796534, whole genome shotgun sequence.
ACCESSION   DAORHF010000265 DAORHF010000000
VERSION     DAORHF010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17768_length_4960_cov_5.796534"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="PKJ16_16270"
     CDS             <1..201
                     /locus_tag="PKJ16_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HNV48601.1"
                     /translation="PVNGRFTAAQRRVYDAVLAAQTAGIEACRAGALFADVHRAAITV
                     LAGFFEEASLPFSELSPGALTP"
     regulatory      complement(510..711)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(920..1633)
                     /locus_tag="PKJ16_16275"
     CDS             complement(920..1633)
                     /locus_tag="PKJ16_16275"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HNV48602.1"
                     /translation="MPVIINGDDFGLRPSIHESIAVLIRRGAMTSASILVLRDPASFH
                     AAARACRDYADRAGFGLHLDLDRWFRFDETRHFGTDESDIDAGYGELVRERRRALHDD
                     IVSQIRTLQDASVPVTHLDGHHHAHLFPGVLEVVVEAMLECGLSRMRFNRAFYRSAES
                     LGRARECVAKNGIRIPDGFVDLADIMRGDAMPCDDDRIIEVMAHTDTTDNEYGRRDQH
                     HYLLAHGACGRALVTFGAL"
     gene            complement(1644..2135)
                     /locus_tag="PKJ16_16280"
     CDS             complement(1644..2135)
                     /locus_tag="PKJ16_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48603.1"
                     /translation="MKGSLAPSTAMSFPLTISAWRRTAGTIALGAFVALSVYLFAKIS
                     DLGIVLFLFKFGLLVAVPLIFVFYEIGVHSYVFSAEGVVIRFIYGRKKTILPENIVRM
                     ALDDEYRTDLVVNWKKSRRHATRTIRISGKLCAPYGFSATGLLDALTRLYGGAAAPDR
                     SVS"
     gene            2369..2827
                     /locus_tag="PKJ16_16285"
     CDS             2369..2827
                     /locus_tag="PKJ16_16285"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HNV48604.1"
                     /translation="MSKITIEFGDLVAALENNSNEHAHYLDLKTGTVIRTVADRFSES
                     IEDTDQIHSMIEQEPYRYLAITPMGSAERYAIIEQFVDHIQDARLKEQLKTPLMKKKP
                     FRTFNDAINNYPEIREKWRAFHDGELTRRSLDWLEENGIDAELANAASAG"
     gene            2846..3292
                     /locus_tag="PKJ16_16290"
     CDS             2846..3292
                     /locus_tag="PKJ16_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48605.1"
                     /translation="MDSFDELRRALEGITPDTPLPREGALADLYHALQDVGEFRACVS
                     DGDLALIYLTFEEVIAESVLDNPGWHIESDERYTVITLLLPDNGSPTPIPLRFDRHDN
                     TSAAFLQHLKSRGRVTVYFLYILYGDLHRGPSPTLLVPTEILSAIP"
     gene            complement(3389..>4960)
                     /locus_tag="PKJ16_16295"
     CDS             complement(3389..>4960)
                     /locus_tag="PKJ16_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV48606.1"
                     /translation="EWGGMGLPEVLFQSCTEYFSAACLAFTMYPMLTIGCANLIKNFG
                     TDELKKIYLEKMYSGQWGGTMCLTEPDAGSDVGALKTKAVKQADGTYLITGTKIFISS
                     GDNDLYENMVHPVLARIEGDPAGTKGISIFVVPKFFPNPDGSLGKQNDVVCAGIEHKM
                     GIKGSATCTLSFGDNGKCVGWLLGEERKGMKIMFQMMNEARLFVGNQGLSVSSAAYMH
                     AITYAKNRIQGAHVTQMLNPDAKGVQIVQHPDVRRMLLWMKSNVEAMRMLSYLAGYSL
                     DFAHAADGEAKKEAQALVEFLIPITKAGNTDLSWLVTAEACQVYGGYGFCGDYPVEQF
                     ARDSKILSIYEGTNGIQSMDLAMRKLLMNPEQYNYSIYKKRIKETCAKAKGVVDDKYI
                     AMVEKGVAKMDEVVNKMKEQMAGGKFLNLFATATPLQVAFRFLTHAWLHLWSLTLATP
                     QMKKLVGDAKGADRDAILKDNAEAAYYAGKVLSAQFYIGAEFHRYFGQIDYLLSDESA
                     VIKTSDEIYTGAPAE"
BASE COUNT          982 a         1581 c         1468 g          929 t
ORIGIN      
        1 ccggtgaacg gccgcttcac ggctgcgcag cggcgggtct acgacgcggt gctcgcggcg
       61 cagaccgccg gcatcgaggc ctgccgggcg ggggccctct tcgccgacgt ccaccgtgcc
      121 gccatcacgg tgctcgcggg cttcttcgag gaggcatcac tccccttctc cgagctctcc
      181 ccgggggctc tgaccccttg atctcctgat ttccctttcc catcctccga ggctctgacc
      241 ccgtcagggg ctctgacccc gggatcctgg ctctgacccc tttgcgggac gagcacacca
      301 ctggcagcta tcgttaaaat caatacaaaa cgagatactc tgttcatgtc atccatctcc
      361 tttccgaaac cgcatgcgga cggctgcgga atcccgccgc accgtggaga atcccatata
      421 acgaaaaaat ccccggacca ttgctggccc ggggattccc attttcatcc acagttacct
      481 gaacgcccat ccagcccatc tccggggagg atcggtccaa tgttacaggc aggtcttctg
      541 gctctcccgc tcttccggcg gccttcccat cgcacatgcg acagtggcgc actgggccga
      601 aagagttccc tcaaacttcg agggcgggat tacagcggcg ggtccgcacc cggtttgcac
      661 gggtttccct tttgtctccg tatttggaga acctgtaatc gttgcgggca tggtgcttca
      721 tcgatggaaa tcgtcaatga aaatttccac gagaaaaaca cggaatcatt gcgtgtttcg
      781 catcgatatc tgacaatcat gggatatcac gcagaaccgc ggacatcccc tcccccgaac
      841 gaagggggag gttcccaaca ggatgttgca atgccgagca tggacaggat gtccaaaaaa
      901 tgctcggccc gggagggggt cagagcgccc caaatgtcac gagcgctcgg ccgcacgcgc
      961 catgtgcaag caaatagtgg tgctgatcgc gcctgccgta ctcgttgtcc gtcgtgtcgg
     1021 tatgcgccat gacctcgatg atgcggtcgt cgtcacacgg catcgcgtcg ccgcgcatta
     1081 tatccgcgag atcgacgaag ccgtccggaa tcctgattcc gtttttcgca acacactccc
     1141 gcgcccgccc gagggactcc gcgctccggt agaacgcgcg attgaagcgc atgcgcgaca
     1201 ggccgcattc gagcatcgcc tcgacgacga cctcgagcac gccgggaaac agatgcgcgt
     1261 ggtgatggcc gtcgaggtgg gtcaccggta cggaggcgtc ctggagcgtg cgaatctgcg
     1321 acacgatgtc gtcgtggagc gcgcgacgac gctcgcggac gagctcgccg tagcccgcgt
     1381 cgatgtcgct ctcgtcggtc ccgaagtggc gcgtctcgtc gaagcggaac cagcggtcca
     1441 gatcgagatg aaggccgaaa cccgcgcggt cggcataatc gcggcaggcg cgcgcggcgg
     1501 cgtggaagga ggcgggatcg cgcaggacca gaatgctcgc gctggtcatc gcgccgcgcc
     1561 ggatcagcac tgcgatactt tcgtgaattg atggcctgag gccaaaatcg tcgccgttaa
     1621 tgatgacggg cacggcgcgc gccttacgaa accgaccgat ccggcgccgc cgctccgcca
     1681 tagaggcgcg taagcgcgtc gagcaatccc gttgccgaaa agccgtatgg cgcgcacagc
     1741 ttcccggaaa tccggatcgt ccgcgtcgca tggcggcggc ttttcttcca gttgacgaca
     1801 aggtccgtgc gatactcgtc gtcgagcgcc atgcggacaa tattctccgg taggatcgtc
     1861 ttttttcggc catagatgaa ccggatgacg accccctccg cagagaagac atacgaatga
     1921 acgccgatct catagaagac aaagatgagc ggcaccgcca cgagcaggcc gaacttaaac
     1981 aagaacaaca cgatgcccag atcgctgatt ttcgcgaaca gatacacgga aagcgcgacg
     2041 aacgcgccga gggctatcgt gcccgccgtt cgcctccaag cggatatggt gagcgggaat
     2101 gacatggccg tgctcggcgc gagcgatcct ttcatgatgg ccccctgcca ttgtcgcaaa
     2161 atctctctgg tgcggtctca ttctccatca cggccgcatg ctgatacgat cggcgccgga
     2221 tcacgctggc aatcattttt cgcacccatt cgcgatggat ttcgccggcg cgccgaatgc
     2281 caatccagaa aatccttgac agcaccgaaa cgccgggacc agtatgcaat accgtatcga
     2341 gaacaatcgc gaaaaacgta taacggctat gtcgaagatc acgatcgaat tcggcgacct
     2401 ggttgcggcg ctcgagaaca attccaacga acacgctcac tacctggacc tcaagaccgg
     2461 taccgtcatc cgcaccgtcg cggaccgctt cagcgagtcc atcgaggata ccgaccagat
     2521 ccacagtatg atcgagcagg agccctatcg ctatctggcg atcaccccga tgggctcggc
     2581 ggagcgatac gccatcatcg agcaatttgt ggaccacatc caggacgcga gactcaagga
     2641 gcagctcaaa acgccgctca tgaagaaaaa accgttccgc accttcaacg acgcaatcaa
     2701 caattatccc gaaatacgcg aaaagtggcg cgcgtttcac gatggcgagc tcacgcggcg
     2761 ctcgctcgac tggctcgagg aaaacggcat cgacgccgag ctcgcgaacg cggcgtccgc
     2821 aggctgaccg gcgcgcacta cgcacatgga cagcttcgac gaattgcgcc gcgccctcga
     2881 gggcatcaca cccgacaccc cgcttccgcg cgagggcgcg ctcgcggacc tataccacgc
     2941 gctgcaagac gtgggcgagt tccgcgcatg cgtctcggac ggggacctcg cgctcatcta
     3001 tctcaccttc gaggaagtga tcgccgaaag cgtgctcgac aatcccggct ggcacatcga
     3061 gagcgacgag cgctacaccg tcatcacact cctcctgccg gacaacggat cgcccacgcc
     3121 catcccgctc cgcttcgacc ggcacgacaa caccagcgcc gcattccttc aacacctgaa
     3181 atcgcgcggg cgtgtgacgg tgtacttcct gtacattctg tacggagacc tccatcgcgg
     3241 cccgtcgccg accctgctgg tcccgacaga aatactttcc gcaataccct gatatcgagc
     3301 gccgcgaaca tcaccaggcc caataaatga aacggcggcg gtaggaaacc ccaccgccgc
     3361 cgccgtcggt caaatctgct tcgtgcgcct attcggccgg cgcccccgta tagatctcgt
     3421 cggaggtctt gatgacggcg ctctcgtccg agaggaggta atcgatctgg ccgaagtacc
     3481 ggtggaactc ggcgccgatg tagaactggg ccgagaggac cttgccggca tagtacgcgg
     3541 cctcggcgtt gtccttgagg atcgcgtcgc ggtcggcgcc cttggcgtcg cccacgagct
     3601 tcttcatctg cggcgtggcc agcgtgagcg accacaggtg gagccacgca tgggtgagga
     3661 agcggaaggc gacctggagc ggtgtcgccg tggcgaacag gttgaggaac ttgccgcccg
     3721 ccatctgctc cttcatcttg ttgacgacct cgtccatctt cgcgacgccc ttctcgacca
     3781 tcgcgatgta cttgtcgtcc acaacgccct tcgccttggc gcaggtttcc ttgatgcgct
     3841 tcttgtaaat gctatagttg tactgctccg ggttcatgag gagcttgcgc atcgcgaggt
     3901 ccatcgactg gatgccgttg gtcccctcgt agatcgaaag gatcttcgag tcgcgcgcga
     3961 actgctcgac cggatagtcc ccgcagaagc cgtaaccgcc gtatacctgg caggcctcgg
     4021 cggtcaccag ccacgagaga tcggtgttgc cggccttggt gatcggaatg aggaactcga
     4081 ccagcgcctg cgcctccttc ttggcctcgc cgtcggccgc gtgcgcgaaa tcgaggctgt
     4141 aaccggccag atacgacagc atccgcatcg cctcgacgtt ggacttcatc cacagcagca
     4201 tgcggcggac atcggggtgc tggacgatct ggacgccctt ggcgtcgggg ttgagcatct
     4261 gggtcacgtg cgcgccctgg atgcggtttt tcgcataggt gatcgcgtgc atgtacgcgg
     4321 cgctggagac cgagaggccc tggttgccca cgaagaggcg cgcctcgttc atcatctgga
     4381 acatgatctt catgcccttg cgctcctcgc ccaggagcca gccgacgcac ttgccgttgt
     4441 cgccgaagga gagtgtgcag gtcgcggaac ccttgatgcc catcttgtgc tcgatgcccg
     4501 cgcacacgac gtcgttctgc ttgcccagcg agccgtccgg attcgggaag aacttgggaa
     4561 cgacgaagat ggagattccc ttggtgccgg ccggatcgcc ttcaatgcgg gcgagcaccg
     4621 gatggaccat gttttcatag agatcgttgt cgccggagga gatgaagatc ttcgtgccgg
     4681 tgatgagata cgtgccgtcg gcctgcttca cggccttggt cttgagcgcg cccacgtcgg
     4741 agccggcgtc gggctcggtg aggcacatgg tgccgcccca ctggccggaa tacatcttct
     4801 cgagatagat cttcttgagt tcgtcggtgc cgaagttctt gatgaggttc gcgcagccga
     4861 tggtgagcat gggatacatg gtgaaggcca ggcacgcggc gctgaaatac tcggtgcagg
     4921 actggaacag gacctcggga agccccatgc cgccccattc
//
LOCUS       DAORHF010000266         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_17974_length_4920_cov_5.020966, whole genome shotgun sequence.
ACCESSION   DAORHF010000266 DAORHF010000000
VERSION     DAORHF010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17974_length_4920_cov_5.020966"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="PKJ16_16300"
     CDS             complement(<1..378)
                     /locus_tag="PKJ16_16300"
                     /inference="COORDINATES: protein motif:HMM:NF025825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HNV48607.1"
                     /translation="MAPVSALENQLAAIANWATVSYKKNLLKELARTIADDERIDDLL
                     EGFYAGEDTLDAGKELPGILCTTDRRIVFFTSERSKPLVREVRFSDIAAIETEKGYAA
                     MKIAIKTHAANTMVFKSFSRESTV"
     gene            complement(406..1176)
                     /locus_tag="PKJ16_16305"
     CDS             complement(406..1176)
                     /locus_tag="PKJ16_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48608.1"
                     /translation="MKLANPDVAKIVLPVILAELQRRGPQDRRLAWTYDSTLKGDTIS
                     FDTFGYRLIVLLRKDGVWITGGSLRRKSDGKGESITTVLERFMAEKDAFFSSTLRGQF
                     DIPRNRTGNFEVGFHPANLDALRYFLNLATHGVYANNCMGTMQLVSAAVQLHSIVDGV
                     SADTVMDDLGRYLPGRMIACPLKGTYSLAKSGDPRSAVACSIHRTNIVDDSDELYRLF
                     STETEMMKVFQSVRFILMTLGFTPEGIDTNILIRDPLD"
     gene            complement(1173..3104)
                     /locus_tag="PKJ16_16310"
     CDS             complement(1173..3104)
                     /locus_tag="PKJ16_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48609.1"
                     /translation="MKRLLTIVLCGVMAIAMVTDGPAQKTKRGKGTQGRTKEAQKKRA
                     SETTSHEKRAPLKAETPVRENTAAEELANKLSTIVLADPELGYRAAVHLRRRGGSAEL
                     IKQSAWTRERRTSLAELFELTSGSLAIEQSLQLMAGRDLSREEYRRDKTVSVGTLPAP
                     QLKPHDFKALLGEKTVSVPESASMIPHDAIAVRCASIKHAIRLSDLAKLFKQQAPFFV
                     ANVGHGDDFEKKLLAQFGLKTPGPLVRKFYGVAIDEMSVMVEDPYLVEGADMAVLFKL
                     GNKFLFKERIGKYRESFKKQVSGAAEGSISYNGFTIHTLTSPNGELRAHTVEHGDYWI
                     IASSAWMARRIIDTIQGKVRSISDNLDYRYLLSVHRGAGDAFVYIGDDFVSKIISARF
                     KIGELRRVNCEANLRMIGFARNLYAIERRAAPVFADLSGAKYYLPELVCPEGGTYDMR
                     DGVPRCSKHGSLGGLLPLSANPVEMVTEGEAQRYRQFVIITIIIGGSTSIPSAFGSIS
                     EKTSRCGRSSCPWLKIANTACCKRSWAARRFSSRPFQSPGKRSCFTRRASCAFSTGTG
                     KTYRMWTMSACAPACSGFARSLGRRTQGSMSTPWRRSATSWIFSSLNGTFPSSYVGTC
                     FVSTCAILSAFCSGMWWFE"
     gene            complement(3120..4166)
                     /locus_tag="PKJ16_16315"
     CDS             complement(3120..4166)
                     /locus_tag="PKJ16_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48610.1"
                     /translation="MDTLEKEILPGIFIITQKGRWGVVRPAVNLYFIAGPDGLLFDAG
                     YGNRSSVRMVTRGIERVVSRCTGRGEPCVLTRVLPSHAHPDHFAGLKKIRRSTGARVL
                     LTETMAGIISSRSAYRRSYRFSASRYYREKRPMRQKLIDALVNPLISFLYELVFGTAF
                     ISNPDEIVPDSGSLTVNGEQWTIVPSPGHSADHIALYSESRGVLLAGDNVLRRIITWL
                     GPPKSDLAAYFATLERYRALPRLDVILSAHGSPVRNPQRRIDEIVKWRTKRIADVQRA
                     VDRSGAEGIGFTGIIDAFYRRKSLAKRFLAEGWVMLTLDYLVTEGAIERFAVNGRVRF
                     RSTAYAPDKQLHTT"
     gene            complement(4241..4313)
                     /locus_tag="PKJ16_16320"
     tRNA            complement(4241..4313)
                     /locus_tag="PKJ16_16320"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4278..4280),aa:Ala,seq:cgc)
     gene            complement(4383..>4920)
                     /locus_tag="PKJ16_16325"
     CDS             complement(4383..>4920)
                     /locus_tag="PKJ16_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV48611.1"
                     /translation="VQRNLGRPPTDEERAKHIERLIELFSVAVTVDPARFAEIPGARD
                     IIAGMRSRDGWVVAIATGCWLESAEFKLRAGRIDHSGITMVTSTDEGMRDRLVGRCIE
                     TARSRAGVRAFDAIVSIGDGRWDVEVASRLGIGFIGVGSDPRSIFRREERCTVIDRYP
                     DVDSFVQLLETAAVPGRE"
BASE COUNT         1010 a         1529 c         1378 g         1003 t
ORIGIN      
        1 gaccgtcgat tcacgcgaga agcttttaaa aaccatcgtg ttcgccgcat gcgttttgat
       61 ggcgatcttc atggcggcat agcccttctc ggtctcgatg gcggcgatgt ccgaaaagcg
      121 gacctcgcgc acgagcggct tcgaacgctc actggtgaaa aacacgatcc gccggtcggt
      181 cgtgcagagg atgccgggaa gctcctttcc ggcgtcgaga gtatcctccc cggcgtagaa
      241 cccctcgaga agatcgtcga tccgctcatc gtccgcgatg gtccgcgcga gctccttcag
      301 gaggtttttt ttgtacgata ccgtcgccca gttggcgatc gcggcgagct ggttctcgag
      361 cgcagagacg ggtgccatat caactcctcc gatgcatttc gcgtatcaat cgagcggatc
      421 tcgaatcagt atgttcgtgt cgatcccctc cggggtgaac ccgagcgtca tcaggatgaa
      481 tctgacagac tggaacacct tcatcatctc cgtttccgta gagaaaagtc ggtacaattc
      541 gtcagaatca tcaacgatgt tcgtcctatg gatcgaacac gcaaccgcac tccgcggatc
      601 tccggacttc gccagggaat acgtgccctt gagcggacag gcgatcattc gaccgggcag
      661 gtagcggccc agatcgtcca tgacggtatc agcggaaacg ccatcgacaa ttgaatgcaa
      721 ttgcaccgca gctgagacga gttgcatcgt gcccatgcaa ttgttcgcat agacgccgtg
      781 ggtcgcaaga ttcaggaaat atcgaagggc gtcaaggttt gcgggatgga accccacctc
      841 gaagttcccc gtacggttcc tcggaatatc gaattgcccc ctgagggtgg aggaaaaaaa
      901 tgcgtccttc tcggccatga accgctcgag cacggtcgtg atgctttcgc ccttcccgtc
      961 cgatttgcgc cggagcgatc cgcccgtgat ccatacgccg tcctttctga gcagcacaat
     1021 caaacgatag ccgaaggtgt cgaacgagat cgtatcgcct ttgagcgttg aatcataggt
     1081 ccaggcgagc ctccggtcct gcggaccgcg ccgctgcaac tccgccagaa tgacgggcaa
     1141 tacgatcttc gcgacgtccg ggttcgccag tttcattcga accaccacat gccggaacag
     1201 aatgcgctca aaatcgcgca tgttgaaacg aagcacgtcc cgacgtaact gctcgggaat
     1261 gtcccgttca atgaagaaaa aatccaactc gttgccgaac gcctccaggg ggtcgacatc
     1321 gagccctggg ttcttcttcc caaactcctg gcgaaacctg agcatgccgg cgcgcacgcg
     1381 ctcatcgtcc acatgcgata ggtctttccg gtacccgtcg aaaaagcgca gcttgcacgc
     1441 cgcgtgaaac acgacctttt tcccggcgat tgaaacggcc tcgagctgaa ccggcgcgcc
     1501 gcccatgatc gtttgcagca agcggtattc gctattttca accaggggca ggatgaccgt
     1561 ccgcaccgag atgttttttc cgaaatcgac ccgaatgccg atgggatcga cgtacttccg
     1621 ccaataatta tggtaattat aacaaattga cgataacgct gcgcctctcc ttcagtaacc
     1681 atttcgaccg ggttcgccga gagcggcaac aatcccccga gcgatccgtg cttgctgcag
     1741 cgagggaccc cgtcgcgcat atcatacgtc ccgccctcgg gacacacgag ttcgggaaga
     1801 tagtacttcg ctccagagag atcggcaaac accggcgccg cacgtcgctc gattgcatac
     1861 aggttgcggg cgaagccgat catccgcagg ttggcctcgc aattgacccg ccgcaattcg
     1921 ccgatcttga accgtgcgct gatgatcttc gatacgaaat catccccgat gtacacgaac
     1981 gcatcgcccg ctccccgatg gacggaaagc aggtagcgat aatccaggtt gtcggagatc
     2041 gaacggacct tcccctgaat cgtgtcgatg atacgacgcg ccatccaggc cgagctcgcg
     2101 atgatccaat agtcgccgtg ctcgacggta tgcgcccgga gctctccgtt gggactggtg
     2161 agcgtatgga tggtgaatcc gttataactg atcgatcctt ccgccgcgcc cgaaacctgt
     2221 ttcttgaagc tctcgcgata cttcccgatc cgctccttga acagaaattt attacccagc
     2281 ttgaagagca cggccatatc ggccccctcg acaagatagg ggtcttcgac catcacgctc
     2341 atctcgtcaa tcgcgacgcc gtagaatttc ctcaccagcg gtcccggcgt tttaaggcca
     2401 aactgggcga gaagtttctt ttcgaaatca tcgccatgcc cgacgttcgc gacgaaaaag
     2461 ggagcctgct gcttgaaaag cttcgccagg tcgctcaatc tgatcgcgtg cttgatgctc
     2521 gcacagcgca ccgcgatcgc gtcgtgcgga atcatggagg cgctctccgg gaccgaaacg
     2581 gtcttttcgc ccaggagcgc cttgaaatca tggggtttca gctgcggggc cggcagcgta
     2641 cccacgctca ccgtcttgtc ccgccgatat tcctcccggg agaggtcgcg cccggccatg
     2701 agctggagac tctgctcgat cgccagcgat ccgctggtga gctcgaagag ctcggcgagc
     2761 gaggttctgc gctcacgagt ccacgccgat tgcttaatca attctgcgct tccgccacgt
     2821 cgccgcagat gcaccgcagc gcgatagccc agctcgggat cggccaaaac gatggtggac
     2881 agtttattcg cgagttcctc cgccgccgtg ttctcgcgaa ccggcgtttc agccttgagg
     2941 ggagcgcgtt tttcatgaga tgtggtttcc gatgcgcgct tcttctgcgc ttctttcgta
     3001 cggccctgcg ttccttttcc gcgtttcgtc ttttgcgccg gcccgtccgt caccatggca
     3061 atcgccatga ctccacagag tacaatcgtc aacaggcgtt tcattgggct cctcccgcat
     3121 caagtagtat gcagctgttt gtctggcgca tatgcggtgg accgaaaccg cacccgcccg
     3181 ttcacggcaa agcgttcaat agcgccttcg gtcaccaggt aatcgagcgt cagcatcacc
     3241 cagccctcgg ccaggaatcg tttcgcaagc gatttcctcc ggtagaaggc gtcgatgata
     3301 ccggtaaagc cgattccttc ggcgcccgat cgatcgacgg cgcgctggac atcggcaata
     3361 cgctttgtgc gccatttgac gatctcgtcg atccgccgct gcggattccg caccggactg
     3421 ccgtgggcgc tcaggatcac atcgagccgc ggtaaggcgc gataccgctc gagcgtcgcg
     3481 aaatacgcgg caaggtccga tttcggcggc ccgagccagg tgatgatcct cctgagcacg
     3541 ttgtcgcccg cgagaaggac gccgcgcgat tcgctgtaca gcgcgatgtg gtcggcgctg
     3601 tgtcccggcg acggaacgat cgtccactgc tctccgttta cggtgagcga cccggaatcg
     3661 ggcacgatct cgtcgggatt ggaaatgaac gcggtcccga acacgagctc gtacaggaac
     3721 gatatgagcg gatttaccag cgcgtcaatc agcttttggc gcatgggccg tttttctcgg
     3781 taatagcgcg atgcgctgaa tcgatatgaa cgccgatagg ccgaccggct cgaaataatt
     3841 ccggccatgg tttcggtgag gagcacccgt gcgcccgtac tgcgtctgat ctttttcaga
     3901 cccgcgaaat gatcaggatg cgcatggctc gggaggacgc gcgtcagtac gcagggctcc
     3961 ccgcgaccgg tacagcgtga aacgacgcgc tcgatacccc gcgtcaccat tcgcaccgac
     4021 gatcggttcc catagccggc atcgaagagc agcccgtccg gccccgcgat gaagtagaga
     4081 tttaccgcgg gacgcacaac gccccagcgc cccttttggg tgatgatgaa aattccggga
     4141 agaatctctt tctcgagggt atccatacgg cgcggcgtta cctgataaac gggcacacaa
     4201 taaaacgccc tccacgagcg tggaaggcgt ttgcgtgaag tggagccgaa gggaatcgaa
     4261 ccctcgacct ctagagtgcg attctagcgc tctcccaact gagctacggc cccgtctgga
     4321 gtaacgacat cgccgagggg gcgtgtattg tcaatcgata tattccgccg tatgccactt
     4381 ttttactcgc gacctggcac ggcggccgtt tcgagcaatt gaacaaacga atcgacgtcg
     4441 ggatagcgat cgatcaccgt acatcgctct tcgcgccgga atatcgatcg ggggtcagag
     4501 cccactccga taaagccgat cccgaggcgg gaggcgacct ccacgtccca gcgaccgtcg
     4561 ccaatactga cgatcgcatc gaaggccctg accccggccc gcgagcgcgc agtctcgatg
     4621 caccgcccca cgagacggtc ccgcatcccc tcgtcggtgc tcgtcaccat cgtgatgccg
     4681 gaatggtcga tgcggcccgc gcgcaacttg aattcggccg actcgagcca gcacccggtc
     4741 gcgatcgcga caacccatcc gtctcgtgac cgcatccccg caataatgtc gcgcgccccg
     4801 gggatctcgg cgaatcgagc gggatcaacc gtaacggcca cgctgaaaag ctcgatcaac
     4861 cgttcgatat gcttcgcgcg ttcctcgtcg gtgggcggtc gcccgagatt tcgctggacc
//
LOCUS       DAORHF010000267         4852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_18335_length_4852_cov_4.610798, whole genome shotgun sequence.
ACCESSION   DAORHF010000267 DAORHF010000000
VERSION     DAORHF010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18335_length_4852_cov_4.610798"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PKJ16_16330"
     CDS             <1..954
                     /locus_tag="PKJ16_16330"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HNV48612.1"
                     /translation="VSACPLPTRMTDAILRGAARARRRPLVAGALSLLVPGLGQLYNG
                     EPAKAVVFALLRALPGLVAAAAAVSDNAPSHLRVWSLAVGGCVAVWLAAPAEAAWASL
                     RRRDFTPGAVNRAAVYVLAGALGTALFVGSIAALGSVFALERAGTDDMRPTIDRTSLL
                     LVRRRAAVSAGDVVLARAESGRFLARVIASDGRRVTVRDGRLSVGGFELPVGVYSAEE
                     LARAGLANRAELFFETNGMREYPILLPAHDRRRAGMRWEPVAGAPPNAITLAFDDRTR
                     GRVCFMVPRDALVGRVEGIVRGPSLRRSLALPWAERAAPTR"
     gene            1031..1999
                     /locus_tag="PKJ16_16335"
     CDS             1031..1999
                     /locus_tag="PKJ16_16335"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HNV48613.1"
                     /translation="MVLVTGCNGLIGKKLIDRLLGDGEPVRGLDFWRDRNLAAGVEFF
                     EGTVLDDELLLDATADVDTVYHLMDIYNTTHNSRRRMRRINTTGTEKLLAAARDNHVR
                     KVVFLSSGEVYGTSDDMPIGIGDPLHPVTRYGKDKVKAEKICQAAIENDGMDITIFRP
                     TIMAGPGIDDPLILIILYMAMAMGDGNRFYVGGDGSSRFQLVHPDDVVDALIRAKDRT
                     GSRGGVYNLGSDNVPTQIEQVERIKKTARVDGAIHVLSPAITRLLSIVLRPFNINYLS
                     KEHVIFILSNFVLDCSTTKTDLGWSPTRDNVAMMAEAVEWYRKEKL"
     gene            2134..2886
                     /locus_tag="PKJ16_16340"
     CDS             2134..2886
                     /locus_tag="PKJ16_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48614.1"
                     /translation="MKYPHVILIALVALLTHCSGLGIVYYSEPNTHAVNERDSILQYK
                     LRGYETRYGNVFQAQVQDLTVAVKVNNERESGLSFGPLMFPIIPLIGFDYGYGFAPRN
                     DINATTSVFITVAFTAPADKEYVFNPCDVEIIKNNAERIKPVGYSDTFNFAYLNKKAV
                     KLNYSEEIKDVDGLTDCSTEGGGIPLTKSTQVLIKFAVPLSPSDTFQVSLPAITSDEH
                     DDVDVPIINFHPKRGIIYSPFVVSTATLRFIY"
     gene            complement(2897..4606)
                     /locus_tag="PKJ16_16345"
     CDS             complement(2897..4606)
                     /locus_tag="PKJ16_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48615.1"
                     /translation="MAAYQTKFSSTERPCTDFFDYWDSLGFFDNLQVVLPPQFFAIFE
                     IARVIFTEEMVSEALTAIEDITKKEMLPTPDRDVSIMVNRMERTSPLSDNMEIDNFQT
                     IYQLRKTLPRELAWDDDIFNMKLLTKSLLVQKFYESETDRFKPVSTRRDESGREANRF
                     EQKFFLLLDRSKSMDMRMRSFYSKSIVAEFLRRKINSNAKIFYRPFDSKPGELFKLEK
                     KEDFPLLIERVLLTTTGGTSTNLQAAVCQAASDINYDKDMANAEILVVTDGISKINKF
                     ELRERLGDIKLNILKVGRDLAEPDFYGVKGAMENVRIDTDFTAYNIQEVNQKMARAQL
                     DEESLSPREKRAYGFMLEYSDSIITDLKYISNRFIEIGDLEPTELFKLDDDILDFIEM
                     TTAQLAQLDLSERSVEDLKVIYKKAYFLGQYIELLMEYGGNIANPVLKKAQQALQEAR
                     QKMLNDPVLYELVTRAGNFREDKKMLKMSRKEARKRMKEMKLEERALSSKEMKQAQLI
                     LTMDTGTSEGGSMGQLIKLLVIKLVQGIVFVVKKIAGARTAPTDSGGSEPPRQQEKGR
                     KAS"
BASE COUNT          841 a         1468 c         1540 g         1003 t
ORIGIN      
        1 gtttccgcgt gtccgctccc gacgagaatg actgacgcga tactcagggg tgccgcgcgc
       61 gcccgccgac gcccgctggt ggccggcgcg ctctcgctcc tggtccccgg cctgggccag
      121 ctctacaacg gcgagccggc gaaggccgtc gtgttcgcgc tgctgcgggc gctcccgggg
      181 cttgtcgccg ctgccgcggc agtttcggat aacgcgccgt cgcacctgcg cgtctggtcc
      241 ctcgcggtgg gcggttgtgt cgcggtgtgg cttgcggcgc cggccgaggc ggcctgggcc
      301 tccctgcggc gccgggactt cacgccgggc gccgtgaacc gcgcggcggt ctatgtcctc
      361 gcgggcgcgc tggggacggc cctcttcgtc ggatcgatcg cagcgctggg gtcggtgttt
      421 gcccttgagc gcgccggaac ggacgatatg cgccccacga ttgatcggac aagccttctg
      481 ctcgtccgcc gtcgggcggc ggtgtcggcc ggggacgtgg ttctagcgcg cgccgagagc
      541 ggccgcttcc tcgcacgcgt gatcgcttcc gacggacgac gggtgacggt gcgcgacggg
      601 cgcctgagcg tcggcgggtt cgagctgccc gttggcgtgt actccgccga ggagctcgcg
      661 cgcgccgggc ttgcgaaccg cgccgagttg tttttcgaga cgaacggcat gcgagagtac
      721 ccgatacttc tgcccgcgca cgaccggcga cgcgcgggga tgcgctggga gcccgtcgcg
      781 ggcgcgccgc cgaacgccat cacgctcgcc ttcgacgacc gcacccgggg gcgtgtctgc
      841 ttcatggtgc ctcgtgatgc actcgtcggc cgcgtcgagg ggatcgtgcg cggcccctcg
      901 ctccgccgtt cgctcgcatt gccctgggcg gagcgcgcgg ccccgacgcg ctgatgcgtg
      961 cggtattcta cttgacgaaa aacgacacat tgctgcatct ttatcgaagc gcgcgacaag
     1021 ggaggatgcc atggtcctgg ttaccggctg taacggcctt atcggaaaaa aattgatcga
     1081 ccgacttctg ggagacgggg aaccggtgcg cggactggat ttctggcggg accggaacct
     1141 cgccgcaggt gtcgagtttt tcgaagggac cgtgctggac gatgagctcc tactcgatgc
     1201 gaccgcggat gtcgataccg tctaccacct catggacatc tacaatacga cgcacaacag
     1261 ccgccgccgc atgcggcgga tcaacacgac gggcacggaa aagctccttg cggccgcccg
     1321 ggacaatcac gtgcgcaagg tggtgttcct ctcctccgga gaggtgtacg gcacgtcgga
     1381 tgacatgccc atcgggatcg gcgatcccct ccatcccgtt acccgctatg ggaaggacaa
     1441 ggtcaaggcc gagaagattt gccaggcggc gatcgagaac gacgggatgg atatcaccat
     1501 attccgtccc accatcatgg ccggtccggg aatcgacgat ccgctcattc tcatcattct
     1561 gtatatggcg atggcgatgg gcgatggaaa ccgcttctac gtgggcggcg acggcagctc
     1621 gcgcttccag ctcgtgcatc ccgacgatgt cgtcgatgcg ttgattcgcg cgaaggaccg
     1681 caccggatcc cgcggcggcg tatacaatct tggctccgac aatgtgccca cgcagatcga
     1741 gcaagtggag cgcatcaaga agaccgcgcg cgtcgatggc gcgatccacg ttctctcccc
     1801 ggcgatcacg cggctgctct cgatcgtcct gcggcccttc aatatcaatt atctcagcaa
     1861 ggaacacgtc atcttcattc tctcgaactt cgtgctcgac tgctccacga caaaaaccga
     1921 cctgggatgg tcgcccacgc gggacaacgt ggcgatgatg gccgaggcgg tcgagtggta
     1981 ccggaaggaa aaattgtaat caatagtggg ggcggatgag gtccccgcgc ctcaccgtcg
     2041 tcgctctcgc gccggcaggc tcccggggtc gcgcatgatg gccgggggga tgcgtcacgg
     2101 tcggcacgcg ttcatcgatg gtgtgtgaat tccatgaaat atccacatgt gatactgatc
     2161 gcccttgtcg cgctcctgac gcactgctcg ggccttggaa tcgtgtacta cagcgaaccg
     2221 aacacgcatg cggtgaatga gcgggactcc attctgcagt acaagctgcg cggatacgaa
     2281 acgcgctatg gcaatgtgtt ccaggcgcaa gtccaggatc tcacggtcgc ggtgaaggtg
     2341 aacaacgagc gcgaatcggg gctgtcgttc ggtccgctga tgtttccgat aatcccgctt
     2401 atcggcttcg attacggata cgggttcgcg cctcgaaacg atatcaatgc gacaacgtca
     2461 gtcttcatca ccgtggcctt cacggcccct gcggacaagg aatacgtgtt caatccctgc
     2521 gacgtggaga tcattaaaaa caacgcggaa cggatcaaac cggtcggata cagcgacacg
     2581 ttcaacttcg cgtatctgaa caagaaagcg gtaaagctta attattccga agaaataaag
     2641 gatgtcgacg gcctgactga ttgctccacg gagggaggcg gcatcccgct gacgaaatcg
     2701 acgcaggtgc tgatcaagtt tgccgtgccc ctgtcgccct cggacacctt tcaggtgtcg
     2761 ctgccggcca tcacgtcgga cgagcacgac gatgtggatg tgccgatcat caatttccat
     2821 cccaaacggg gaatcatcta ttcgcccttc gtggtgtcga cggcgaccct gcggtttatc
     2881 tactgatcgg gagaccctag ctcgccttgc gtcccttctc ttgctgtcgt ggcggttcgc
     2941 ttcccccgct gtcggtgggc gccgtgcgcg cgcccgcgat cttcttgacc acgaacacga
     3001 tcccttgcac gagctttatc accaggagct ttatcagctg gcccatgctc ccgccctcgg
     3061 aggtgcccgt gtccatcgtc aggatgagct gggcctgctt catctccttg gaggaaagcg
     3121 cgcgttcctc gagcttcatt tccttcatgc gcttgcgcgc ctccttgcgc gacatcttga
     3181 gcatcttctt gtcttcgcgg aagttccctg cgcgcgtcac cagctcatag aggaccgggt
     3241 cattgagcat cttctggcgg gcctcctgga gcgcctgctg cgcttttttc aggacggggt
     3301 ttgcgatgtt cccgccgtat tccatcagga gctcaatgta ctggccgagg aaatacgcct
     3361 tcttgtagat gaccttcagg tcctccacgg agcgttcgga gaggtccagc tgcgcgagct
     3421 gggcggtggt catctcgatg aaatcaagga tgtcgtcgtc gagcttgaag agctccgtgg
     3481 gctccaaatc gccgatttcg atgaagcgat tcgatatata cttgaggtcc gtgatgatgc
     3541 tgtcggagta ttcgagcatg aagccgtagg cgcgcttttc ccgcggggag agcgattcct
     3601 cgtcgagctg cgcccgcgcc atcttctggt tgacctcctg gatgttatac gcggtgaagt
     3661 ccgtgtcgat ccggacgttt tccatcgcgc ccttcacgcc gtagaagtcg ggctcggcga
     3721 gatcgcgccc caccttgagg atgttcaact tgatgtcgcc cagccgctcg cgcagctcga
     3781 acttgttgat cttgctgatg ccgtcggtga ccaccaggat ctcggcgttg gccatgtcct
     3841 tgtcgtagtt gatgtcgctt gccgcctggc agacggccgc ctgcaggttg gtgctggtgc
     3901 cgcccgtcgt ggtcaggagc acccgctcga tcaagagcgg gaaatcctcc ttcttctcga
     3961 gcttgaaaag ctcgccgggc ttcgaatcga aggggcggta gaatattttc gcgttcgagt
     4021 tgatcttccg gcgcaggaac tccgcgacga tgctcttcga gtagaacgag cgcatccgca
     4081 tgtccattga cttggagcgg tccagcagga ggaaaaactt ctgctcgaag cggttcgcct
     4141 cccgtcccga ctcgtcgcgg cgcgttgata cgggcttgaa gcggtcggtc tcggactcgt
     4201 agaacttctg caccaggagc gacttggtga ggagcttcat gttgaagatg tcgtcgtccc
     4261 aggcgagctc gcggggcagg gtcttcctga gttggtagat ggtctggaag ttgtcgatct
     4321 ccatgttgtc gctcaggggc gaggtccgtt ccatccggtt gaccatgatg gagacgtcgc
     4381 gatcgggcgt cggcagcatt tcctttttgg tgatgtcctc gatcgccgtg agcgcctccg
     4441 acaccatctc ttcggtgaag atgacgcggg cgatctcgaa gatcgcgaaa aactgcggag
     4501 gaaggaccac ctggaggttg tcgaaaaagc ccaggctgtc ccagtaatcg aagaaatccg
     4561 tgcaggggcg ttcggtgctg ctgaactttg tctggtatgc cgccacgacg gtgccgggcc
     4621 tacaggtccc tgaagtcctt gagcaggcct tccttgagct cgcgcacggc cgagccggcg
     4681 ttgcgccgcc atctggcagc gcggaaggca gggcacatat aatggcacgt atatgtgggg
     4741 tgacctgcag aggagtccgt ggtggcccag atcacgctgt atgtcgacga acagacccgg
     4801 gagcggctcg agcaggctgc cgcgcgacga gcggtgtcgc agagccgcta tg
//
LOCUS       DAORHF010000268         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_18421_length_4835_cov_4.710251, whole genome shotgun sequence.
ACCESSION   DAORHF010000268 DAORHF010000000
VERSION     DAORHF010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18421_length_4835_cov_4.710251"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="PKJ16_16350"
     CDS             <1..1041
                     /locus_tag="PKJ16_16350"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HNV48616.1"
                     /translation="ARIQKAYKYDDISDAFDDVLDDSEGAGLGLIMALMLCKSAGFPN
                     DGFKIARKGNLTIAIITIPQHIQKKELHTKITDEIIAEIDRIPSFPENIVAIQRLCAN
                     PKSSVREIAEHIRRDPGLTANILKLANSAGYITMNKVETIEGAVKIIGLKGINALLIA
                     SGVQRIMDSRYKKFEEVWKSSNRSAFYAQKIAMQMKRVRLAELVYLAALLSDLGRIVM
                     LSIRPDLGKRIKELVGIKGIGVSSVLEEMSLGMSHSTLGCKVLEKWNFNKTLISVIEY
                     HHRPYMAPDEIRDLVYIVYLANTLADIEKIGEKPYPIDSEILQYFDLTEESDLILLHQ
                     SLSDAYASQTEE"
     gene            1098..2009
                     /gene="glyQ"
                     /locus_tag="PKJ16_16355"
     CDS             1098..2009
                     /gene="glyQ"
                     /locus_tag="PKJ16_16355"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HNV48617.1"
                     /translation="MYFQDIIARLHEYWAAHGCIVIQGYDLEVGAGTFNPATFLRALG
                     PEPWNVAYVEPSRRPTDGRYGDNPNRLQHYYQYQVIMKPSPLDIQDLYLDSLRALGIR
                     LEEHDVRFVEDDWESPTLGAAGLGWEVWLDGMEITQFTYFQLCGSIELNPIPVELTYG
                     LERICMYIQGKDSVFDIQWNDRLTYRDVHHRSEFEFSHYNFNLSDVALHFTLFDLYEK
                     ECLAILDNPAIRVVLPAYDYVLKCSHAFNMLDARGAISVTERVGYIARVRTLARRCAE
                     RYLEIREEMGFPLLNNAAARTAVARNA"
     gene            2024..3007
                     /locus_tag="PKJ16_16360"
     CDS             2024..3007
                     /locus_tag="PKJ16_16360"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HNV48618.1"
                     /translation="MPDLHVLEEYLSRHDRFIISTHESPDGDGLGAEIAFRELLLHLG
                     KEAIILNSDATPEKFLFVDQENEVNVFSKGCEIPDNLAEYAVFVLDTNDFENIGSVHP
                     IIKDLDLLIIDHHEVNESRGSSRGHYIAVDASSTSEIIYTLMRHFGAEPSRKAAQAIY
                     TGILYDTGSFRYPKTSPATFRAIADLVERGANPFNIYEQVYENNSLASFELRALMLAS
                     MEKYFDGRLILMRLTPEMLKESGASFSEGELNINLPHTVKGVVASILVKQDIGGPIKV
                     SMRTKGDYDVAKIAMANGGGGHKNAAGYKSKLSLDETCRKVLQEMQHFFIL"
     gene            complement(3002..3943)
                     /locus_tag="PKJ16_16365"
     CDS             complement(3002..3943)
                     /locus_tag="PKJ16_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HNV48619.1"
                     /translation="MIAPPDATRATLPLCDIEIDDARFRLGAPVPDQRLVDSIRRIGV
                     LRPPVVLEGAPAALPVFGHGRLRALRELAVERAEVVRVRAVDPEWYLRAALDRHYRQA
                     LGPVARLRLVRLLRREFTMDERRIEPIARAELGVPDALAGDDAAASVLSLPPELLDYL
                     DHRAIGYKTIRELVGLDPAAIALLAGWIGATDMRVNVFKEIVEMLGDLTTRPEVLRAL
                     SVETDPSMDRRACERAIHEAVFCARYPDYAASRERANNSLRALTVPGVEVVFPEFFEG
                     NTVEVRIRLGRRDGASVLRERIGRIDDDALARLLELL"
     gene            complement(3949..>4835)
                     /locus_tag="PKJ16_16370"
     CDS             complement(3949..>4835)
                     /locus_tag="PKJ16_16370"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HNV48620.1"
                     /translation="AATTPDPQVKFVALRLDAFIHLQSKRHDEGVKVFQDLLEMARTD
                     EFQDELPLVLYDIGFACIRAEKSRMAYDYWNELYQVDKSFQRIDGLITMLRKEMDVDY
                     KSIKETFDVSVADYIDEWLEKAFPATFLWDLCGLKSAETINLKDVIVTTRVSAGGGGA
                     DLSSYRDYSEALARLSALDTESFRVMANRVVQKLGYKVDQILPTYRDTDGVDFMTVSV
                     STKEKTLIWVRRWTKITVGEIPLRNLAQSTNDLKARQGILITAAELTPAAEESLSRLS
                     KVSVVHPDQLGEILDGLL"
BASE COUNT          953 a         1601 c         1448 g          833 t
ORIGIN      
        1 gcccgcatcc agaaggcgta taaatacgac gatatctccg acgccttcga cgacgtcctg
       61 gacgactcgg agggagccgg cctggggctc atcatggcgc tcatgttgtg caagagcgcc
      121 ggcttcccca acgacggctt caagatcgcg cgcaagggca atctcaccat cgccatcatc
      181 accatacccc agcacatcca aaagaaggag ctccacacca aaatcaccga cgagatcatc
      241 gccgagatcg accgcatccc ctcgttcccc gaaaacatcg tggccatcca gcgcctgtgc
      301 gccaacccga agtcgtcggt gcgcgagatc gcggagcaca tccgacgcga ccccgggctc
      361 accgcgaaca tcctgaagct ggccaactcc gcgggctata tcaccatgaa caaggtggag
      421 acgatcgagg gcgccgtgaa gatcatcggc ctcaagggga taaacgccct cttgatcgcc
      481 agcggcgtcc agcgcatcat ggactcgcgc tacaagaagt tcgaggaggt gtggaaatcg
      541 tcgaaccgct cggccttcta cgcccagaag atcgccatgc agatgaagcg cgtgcgactg
      601 gcggagctgg tgtacctggc ggcgctcctg tccgacctcg gacgcatcgt gatgctctcc
      661 atccggcccg acctgggcaa gcggatcaag gagctcgtgg ggatcaaggg gatcggcgtc
      721 tccagcgtcc tcgaggagat gagcctgggg atgagccaca gcacgctggg ctgcaaggtg
      781 cttgagaaat ggaacttcaa caagaccctg atcagcgtga tcgagtatca tcatcggccc
      841 tatatggcgc cggacgagat acgcgatctc gtctacatcg tgtacctggc gaacacccta
      901 gccgatatcg agaagatcgg cgagaagccc tatcccatcg attcggagat cctccagtac
      961 ttcgacctca cggaggagag cgacctcatc ctcctccatc agtcgctctc ggacgcctac
     1021 gccagccaga ccgaggagtg attattcgca aataaagtac ttgactgaaa tcgcccgcgc
     1081 gacacccttc tatcgatatg tatttccagg atatcatcgc tcgcctgcac gagtactggg
     1141 ccgcacacgg ctgcatcgtc atccaggggt acgacctgga ggtcggcgcg ggcaccttca
     1201 atcccgcgac ctttttgcgg gcgctgggcc cggagccgtg gaacgtcgcc tacgtcgagc
     1261 cctcgcgccg gccgaccgac ggtcgctacg gagacaaccc caaccgcctg cagcactact
     1321 atcagtacca ggtgatcatg aagccctcgc cgctggacat ccaggacctg tatctggact
     1381 cgcttcgggc gctcggcatc aggctcgagg agcacgacgt ccgctttgtc gaggacgact
     1441 gggaatcgcc cacgctgggc gccgccggcc tcggctggga ggtgtggctg gacggcatgg
     1501 agatcacgca gttcacctat ttccaactct gcggaagcat cgagctcaac cccataccgg
     1561 tcgagctcac ctacggcctc gagcggatct gtatgtacat tcagggcaag gacagcgtgt
     1621 tcgacatcca gtggaacgat cgcctcacct atcgcgacgt gcaccatcga agcgagttcg
     1681 agttctcgca ttacaacttc aacctgtccg atgtcgcgct gcacttcacg ctgttcgatc
     1741 tgtacgagaa ggagtgcctg gcgatcctcg acaatcccgc gatccgcgtg gttctgccgg
     1801 cctacgacta cgtcctgaag tgctcgcacg ccttcaacat gctcgatgcg cgcggcgcca
     1861 tctccgtgac cgagcgcgtg ggctacatcg cccgggtgcg caccctggcg cggcgctgcg
     1921 ccgagcggta tctggagatc cgcgaggaga tggggttccc gctcctgaac aacgccgccg
     1981 cacgaactgc cgtcgcgcgg aacgcctgag gctgcgtccg gccatgcccg atctccacgt
     2041 ccttgaagag tacctgtccc gtcacgaccg atttatcatc tcgacccatg aaagtcccga
     2101 cggcgacggc ctcggcgccg agatcgcctt ccgcgagctt ttgctgcacc tgggcaaaga
     2161 ggccatcatc ctcaattccg acgccactcc ggaaaagttc ctgttcgtcg accaggagaa
     2221 cgaggtgaac gtgttcagca agggctgcga gatccccgac aacctcgcgg agtacgcggt
     2281 cttcgtcctg gacaccaacg acttcgagaa catcggatcg gtgcatccca tcatcaagga
     2341 cctggacctg ctcattatag accaccacga ggtgaacgag tcgcgcggct cgtcgcgcgg
     2401 gcactatatc gcggtggacg cctcctccac cagcgagatc atctacaccc tcatgcggca
     2461 cttcggcgcc gagccgagcc ggaaggcggc gcaggcgatc tacacgggca tcctgtacga
     2521 tacgggctcg tttcgctacc cgaagacctc gcccgcgacc ttccgcgcca tcgccgacct
     2581 ggtcgagcgc ggcgcgaacc ccttcaatat ctacgaacag gtgtacgaga acaactccct
     2641 ggccagcttc gagctgcgcg cgctcatgct cgcgtccatg gaaaagtact tcgacggtcg
     2701 gctcatcctg atgcgcctca cgcccgagat gctcaaggag tcgggcgcgt cgttctccga
     2761 gggggaactc aacatcaacc tcccccacac ggtaaagggc gtggtcgcga gcattctggt
     2821 gaagcaggat atcggcgggc ccatcaaggt gagcatgcgg accaagggcg actacgacgt
     2881 cgcgaagatc gccatggcca acggcggggg ggggcacaag aacgcggctg gctataaatc
     2941 gaaactgtcc ctcgacgaga cctgccgcaa ggtgctccag gagatgcagc actttttcat
     3001 tttatagcaa ctccaggagc cgcgcgagcg cgtcatcgtc gatgcgcccg attcgctcgc
     3061 gcaataccga ggcgccgtcc cgacgcccca ggcggatccg cacctcgacc gtattccctt
     3121 cgaaaaattc gggaaacacc acctccaccc ccggcacggt aagcgcgcgc agcgaattgt
     3181 tcgcgcgttc gcgactggcg gcatagtccg gatagcgcgc gcaaaacacg gcctcgtgaa
     3241 tggcgcgctc gcaggcgcgg cggtccatcg acgggtcggt ctccacggaa agcgcgcgga
     3301 ggacctccgg ccgggtagtc agatcgccga gcatctccac gatctccttg aacacgttca
     3361 cccgcatgtc cgtcgcgccg atccagccgg ccaggagcgc gatcgcggcc ggatcgagcc
     3421 cgacgagctc gcggatggtc ttatatccga tcgcccggtg gtcgagatag tccaagagct
     3481 ccgggggaag cgacaggacg ctcgcagccg catcgtcgcc tgcgagcgca tccgggaccc
     3541 ccagctccgc gcgggcgatt ggttcgattc ggcgctcatc catggtaaac tcgcggcgca
     3601 gcaggcgaac gagccgcagg cgcgccaccg ggcccaatgc ctgccgatag tgccggtcca
     3661 gcgcggcgcg caggtaccac tcggggtcga ccgcgcgcac acgcacgacc tccgcgcgct
     3721 cgaccgcaag ctcccgcagc gcgcgcaatc gtccgtgacc gaacaccggg agggccgccg
     3781 gggcgccttc gagaacgacc ggcgggcgca acacgccgat ccggcggatg gagtcgacca
     3841 gccgctgatc cggaaccggg gcgccgagtc gaaagcgcgc gtcgtcaatt tcgatgtcgc
     3901 acaacggcag ggtcgcgcgc gtggcgtcgg gcggggcgat catgcgcgct acagcaatcc
     3961 gtccaggatc tcgcccagct ggtccgggtg caccacggac accttggaga ggcgggagag
     4021 gctctcctcc gcggccgggg tgagctccgc ggccgtgatg agaataccct gccgcgcctt
     4081 gaggtcgttg gtcgactgcg ccaggttccg aagcggtatt tcgcccaccg tgattttcgt
     4141 ccagcgacgc acccagatga gcgttttctc cttcgtggac accgacaccg tcatgaaatc
     4201 aacgccgtcc gtgtcccggt aggtcggaag gatctggtcc accttatacc ccagcttctg
     4261 gaccacgcgg ttggccatca cccggaagga ctcggtgtcc agcgcggaca ggcgggccaa
     4321 tgcctcggag taatctcgat acgaggagag gtccgcgccg ccgccgcccg cgctcacgcg
     4381 cgtcgtgacg ataacgtcct tcaggttgat ggtctcggcg ctcttgagcc cgcagaggtc
     4441 ccatagaaag gttgccggga aggctttctc cagccattcg tcgatgtaat ccgccaccga
     4501 gacatcgaag gtttccttga tgcttttata atcgacgtcc atctccttgc ggagcatggt
     4561 gataaggccg tcgattcgct ggaagctctt gtccacctga tacagctcgt tccagtaatc
     4621 gtaggccatg cgcgatttct cggcgcgtat gcaggcgaag ccgatgtcgt agagcaccag
     4681 gggcagctcg tcctggaact cgtcggtccg ggccatctcg agaaggtcct ggaacacctt
     4741 caccccctcg tcgtgccgct tcgactgcag atggatgaac gcatcgagcc ggagggcgac
     4801 gaacttcacc tgcgggtccg gcgtcgtggc ggcaa
//
LOCUS       DAORHF010000269         4816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_18529_length_4816_cov_5.917664, whole genome shotgun sequence.
ACCESSION   DAORHF010000269 DAORHF010000000
VERSION     DAORHF010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18529_length_4816_cov_5.917664"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PKJ16_16375"
     CDS             <1..214
                     /locus_tag="PKJ16_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48621.1"
                     /translation="SLTENLEGGSLDGMALGDVLRLLDDMSARGILDPDIVGTVKRHS
                     AMMYDTVLAVENESKEFYATRIRASA"
     gene            complement(570..1187)
                     /locus_tag="PKJ16_16380"
     CDS             complement(570..1187)
                     /locus_tag="PKJ16_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48622.1"
                     /translation="MGFFGNLIGSVDSEVIANGILGRGEITNVSISSVTTRNSNGLVH
                     RKCTFSMNVMIDNVAPFQANAVQRIPEIALSKITKGALVPVRVDPGDHGKVFIDFASE
                     LPTVTVPKSAGGSSAAHILEHGKPVKVVFVGSQPMKLKNADGIEMFLLTLTVYEGVDT
                     PYQVQVGNPAPPSALPLMYPGSKLHAKLGNGPNDVVVDWAAGQAT"
     gene            1583..2017
                     /locus_tag="PKJ16_16385"
     CDS             1583..2017
                     /locus_tag="PKJ16_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48623.1"
                     /translation="MIRKFLLIPLLVISACSARSTASTVLNAWTSGDIHVFLAGFEAA
                     AMSHDDAAIMHFFDNSYIQEQMVVNLEGRREQFLNELFGGNFKEILTVRATVLPDRAD
                     PSQPLAATILVTFKSGIQQTMHMDLVWAQRGGRTFALRGPMG"
     gene            2027..4138
                     /gene="tgt"
                     /locus_tag="PKJ16_16390"
     CDS             2027..4138
                     /gene="tgt"
                     /locus_tag="PKJ16_16390"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HNV48624.1"
                     /translation="MPRRYFNDFMPGRHYERSASTYNAPTCPRERTEMQNVTEERLQF
                     TVDSTAVGSSARAGRFTTLHNEVLTPVFMPVATRAVLRTQDTPFVDALGYPVLLANTY
                     HLLLSPGAEVFKACGGIHRFMNWPRSVLTDSGGYQIFSLARSSRITEAGATFRSYLDG
                     KRFLLSPEVSIETQRTIGSDIMMALDYCISSRSDESLSGNAVEVTARWAERSFNARGD
                     SPQSLFGIVQGACFPELRRTSARQITSLPFDGFAIGGLAVGETNEERTEITELTASLL
                     PANRPRYLMGVGTPLDLLEAVHRGVDMFDCILPTSLAQQGVAFTSRGRIRLRRGVHRL
                     SDRPLDEACDCPACSTYSRAYLHHLIKSGEYFGSNLVGRHNLTFYHRLMRVMRERIVQ
                     GDFSDFYRVQRETLGRVDDEDPGRPPRAKKRTETLVLGDYEIIRHDAGFYRIRQRSSG
                     EIMHSVNDPVAESRALYAGQAGLAHVLAQPGDEFVIWDVGLGAGTNAMVALFECQNIL
                     QNSASARRVRIVSFENDLDSFTLAVRNPSRFPHVRHAAPSALLRHGRWMSETVPLEWH
                     LRAGDFPVEMMRAPRPDCIFFDPFSQKTNGLLWSYRVFSSMFQLCKPCGTKLLTYSNS
                     TRVRSSLLAAGFHVGRGAATGPKSETTIAYSQPMAGMELLGREWLERFRRSDARFCEY
                     SSEEDRIAIEKLVESHPQFTI"
     gene            complement(4397..>4816)
                     /locus_tag="PKJ16_16395"
     CDS             complement(4397..>4816)
                     /locus_tag="PKJ16_16395"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HNV48625.1"
                     /translation="GYFHNSQVVAVDHHKKTVTFRFKSWVDRNTKEKSYSTVTMDIYE
                     FMARMLYYLPDRHRKMIRYYGLYAHKVGEKLKIIDRKTWAAAIENSFNKNPELCPDCG
                     STMRPSLVFSFFAEEEMKKLWRSHVLCKGYFRMKRGP"
BASE COUNT          992 a         1439 c         1335 g         1050 t
ORIGIN      
        1 atcgctcacg gagaacctgg aaggtggctc cctcgacggc atggccctcg gcgacgttct
       61 ccgcttgctc gacgacatga gcgcgcgcgg catcctcgat ccggacatcg tcggaacggt
      121 gaagcgtcat tccgccatga tgtacgacac ggtcctcgcc gtcgaaaacg agtccaagga
      181 attctacgcg acgcgcatcc gcgcatcggc ctgatcgacc cgttgcggac cgcatccgac
      241 cgaatatctc cccatccccc acacacgctt tagctccgat ttcgacaaca gcatgcgaac
      301 cgcctgctat tcgaagatcg ctagaaaacg gtattgtctg ccagcagtat cgctttccgt
      361 aatggcgccg cgcaatcaac agccgatcag gacgctatag gggaacgctc ggcatagacg
      421 ccgtccccgg accccggcca ggggacggcg tgggccagga tcacggtatc cgatttgatt
      481 gcgccgtgaa taacgtcctc catcaatcaa ggcgagtacg ttatgtgata tataaatcga
      541 aaccgcacca acccattccg tcgcgaattt tacgtcgcct gccctgccgc ccaatcgacg
      601 acaacgtcat tcggcccgtt cccgagtttg gcatgaagtt tcgagcccgg gtacatcagc
      661 ggcagggccg aggggggcgc cggattgccg acctgcactt gatacggggt atcgacgccc
      721 tcgtagaccg tgagcgtcag gagaaacatc tctatgccgt cggcgttttt gagcttcatc
      781 ggctggctgc cgacaaagac caccttgacc ggcttgccgt gttcgaggat gtgggccgcg
      841 ctgctccccc cagcactctt gggcacggta accgttggca gctcggacgc gaaatcgata
      901 aacaccttgc cgtggtcccc gggatcgaca cgcacgggga ccaacgcgcc cttcgtgatc
      961 ttcgagaggg cgatctccgg aatccgctgg actgcgtttg cctggaacgg cgcgacgttg
     1021 tcaatcatca cgttcatgga gaacgtgcac ttgcggtgga caagtccatt actgttccgc
     1081 gtcgtcacgc tggatattga cacattcgtg atttcacctc tgccgagtat cccgttggcg
     1141 ataacctcag agtctaccga accgatcaaa ttcccgaaaa agcccatagg tgcctccttc
     1201 cttttttatt tgggaatccc ccactacgcg cacatcgaaa agacagaaaa cgacaaaagg
     1261 tacatcaccg cgcaggaaat tgccagtctt ttatcatctg ggattatcca cgccagcctg
     1321 gctccctggg gatggatgct gcgcaatcga cagctgttcc atcgcgcagc cggcgccttc
     1381 ctcctggcgc gaaacggatc gcccttttta ttggttgagt gtaagcgatc gaacacccaa
     1441 ccctccccgg cgttcactac aatccaatcg cggctgcacc ttcccgcagc gcagcgcgca
     1501 ccccccccca agaaacggaa cattcacttg acagaattca tccatccgcg tcccatcatg
     1561 ctcgctggta tgcgaggtat gcatgatacg aaaatttctt ctcattcccc tgctcgttat
     1621 ttcggcgtgt tcggcccggt cgacggcgag caccgtccta aacgcctgga cctccggcga
     1681 catacatgtg tttcttgccg gttttgaggc ggccgcgatg tcgcatgacg atgccgctat
     1741 catgcatttc tttgataaca gctacataca ggaacagatg gtggtgaatc tcgagggacg
     1801 cagggagcag tttctcaatg agctcttcgg cggtaatttc aaggagattc tcactgtacg
     1861 cgcgactgtc ctaccggacc gagcggaccc ctcccaaccg cttgcggcga ccatccttgt
     1921 tacctttaaa agcggaatac aacagaccat gcacatggac cttgtatggg cacagcgcgg
     1981 ggggaggact ttcgccttga gggggcccat ggggtgatgc gtctctttgc cgcgacgcta
     2041 cttcaacgac ttcatgccgg gacgccacta cgaacgcagc gcatcgacct acaatgcacc
     2101 gacatgcccg agggaacgga ccgagatgca aaacgtcacc gaagagcgat tacagttcac
     2161 agttgactcg accgcggttg gctcgtcggc acgggcgggt cgtttcacca ctctccacaa
     2221 tgaggtgctc actccggtgt ttatgccggt cgccacgcgc gcggtcctgc ggacgcagga
     2281 caccccgttc gttgacgcat tggggtatcc cgtgctgctc gcgaacacct accacctcct
     2341 cctgagcccc ggcgccgagg tcttcaaggc atgcggcggc atccaccggt ttatgaactg
     2401 gccgcgatcc gtcctgaccg attccggcgg ttaccagatt ttttccctgg cgcgctcctc
     2461 gcgcatcacc gaggcggggg cgacgttccg cagctatctg gacgggaagc ggtttctcct
     2521 gagcccggag gtgagcatcg agacgcagcg gacgatcggc agcgacatca tgatggcgct
     2581 cgattactgc atttcctcgc ggtcggatga atccttgagc ggaaacgcgg ttgaggtcac
     2641 cgcgcgatgg gccgaacgca gtttcaatgc gcggggcgat tcgccgcaat cgctcttcgg
     2701 cattgtccag ggggcgtgtt tcccggaatt gcgtcgtacg agcgcccgac agatcacctc
     2761 cctgccgttc gacggcttcg caatcggtgg gctcgcggtc ggcgagacaa atgaagagcg
     2821 cacggagatt accgagttaa ccgcctcgtt gctgcctgcc aatcgtcccc gatatctgat
     2881 gggggtcggg accccgctcg accttctcga ggcggtgcat cgcggcgtcg acatgttcga
     2941 ctgcatcctg ccgacctccc ttgcccagca gggagtcgcc ttcacgtcac ggggtaggat
     3001 caggttgaga cggggggtcc atcgcctgtc ggaccgtccg ctcgacgagg cgtgcgattg
     3061 cccggcatgc tccacgtatt cgcgcgccta tcttcaccat ctcatcaagt ccggggaata
     3121 cttcggctcg aacctcgttg gccggcacaa ccttacgttt taccatcggc tgatgcgcgt
     3181 tatgcgagag cggatcgttc aaggcgattt tagcgacttc tatcgggttc agcgggagac
     3241 gctgggaaga gttgacgatg aagatcccgg cagaccgccg cgagcgaaaa agcgcacgga
     3301 aacgcttgtg ctcggcgatt atgaaatcat ccggcacgat gccgggtttt accgcatcag
     3361 gcagcggagt tccggcgaga tcatgcattc ggtcaacgat ccagtggccg aatcccgcgc
     3421 gctgtatgcc ggtcaggccg ggcttgcgca tgtgctcgcg caacccggcg atgaattcgt
     3481 tatatgggat gttgggcttg gcgccggaac caacgcgatg gtcgcgctgt tcgagtgtca
     3541 gaacatactg caaaatagcg cttcggcgcg gcgcgtcagg atcgtcagtt ttgaaaatga
     3601 cctggactcc tttacgctgg cggtgcgcaa cccgtcccgt ttcccgcacg tgcgtcatgc
     3661 cgccccatcg gcgcttttgc gccacgggcg ctggatgtcc gagactgttc cgctggagtg
     3721 gcaccttcgt gcgggggact tccccgtcga gatgatgcgt gcgccgcgac cggattgcat
     3781 tttttttgat ccattctcgc aaaaaacgaa cgggctcctg tggagctatc gagttttctc
     3841 gagcatgttc caattatgca agccctgcgg gacaaagctg ctgacctatt cgaattcaac
     3901 acgcgttcgg agttcgctgc tcgccgccgg gtttcatgtc ggtcgggggg cggcaaccgg
     3961 tcccaagtcg gaaaccacga tcgcgtattc acaaccaatg gcaggaatgg aattgcttgg
     4021 cagggagtgg ctggagcgct ttcgaagaag cgatgcccgg ttttgcgaat acagttcgga
     4081 ggaagatcgc atcgccattg aaaagctggt ggagtctcac ccgcaattta ccatctagga
     4141 aattccgccc ctttccgcaa tgcgttacga gacatactgc cggttatgag aactggatca
     4201 gcccgacaga cagtcctctt acagccaaaa agcgtgtgac atcggtctta aatttacgcg
     4261 gacggggaaa aattgaaaaa ttttgcgagc gatgcgcgtg cggtcccatg ccgcctcgtc
     4321 cgttcaaatc atgcagcttg ggaaaagtca acatgttttt aacccctgaa acagggttca
     4381 gtccaagcgt aactcatcac ggccctcgct tcatccgaaa ataccctttg cacagaacat
     4441 gggaccgcca cagctttttc atttcctctt ctgcgaagaa cgaaaagaca agcgacggcc
     4501 tcattgtact cccgcagtcg ggacagagtt cagggttttt attgaatgaa ttctcaatgg
     4561 ccgctgccca ggtctttcga tcaatgatct tcaacttctc tccgaccttg tgagcataca
     4621 atccgtagta acggatcatt tttcgatggc ggtcgggtaa gtaataaagc atccgtgcca
     4681 taaattcgta gatgtccatc gttaccgtgg agtagctctt ttccttagtg tttcggtcca
     4741 cccagctctt gaatcggaat gtcacggtct ttttatgatg gtcaacagcg acgacctgac
     4801 tgttgtgaaa gtaccc
//
LOCUS       DAORHF010000270         4795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_18640_length_4795_cov_4.400422, whole genome shotgun sequence.
ACCESSION   DAORHF010000270 DAORHF010000000
VERSION     DAORHF010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18640_length_4795_cov_4.400422"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2188
                     /locus_tag="PKJ16_16400"
     CDS             <1..2188
                     /locus_tag="PKJ16_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48626.1"
                     /translation="ELFEMDVHALRAPESATGLEDQVAAVTRDGGSVYETVHRRKDGS
                     TFPVEVSARLIEVLEKRFYQAIIRDISERKRIENALWESQRMYATLVSSLPGFVYRCR
                     NDRKWTMAFISDGCYAITGYTPEDFIDNRTIAFNDIVEHERRDELWSKWQDALAAREE
                     FEDSYRIITKSGLYRWVWERGRGVFADSGELLFLEGFITDVTALKESESNLQKATERF
                     QTAFEYAPTGMTITGTDGRFIDCNTAFCAMLGYTREELAGIPFNDITHPDDRDISREQ
                     LLRVIAGTIDSAIFEKRYLHKSGAVIWVLLSAALVKTPDPQDSYCIAHMIDITQRKRA
                     EDQLRLDEMRFSALYELGRMQNTSEKDIIDFALEEGVRLTGSEVGYAHFIEDDRISLE
                     LFTWSRAALERCTAEKMRHYPVESAGIWADSLRTKRVVFHNDYPNHPDRKGYPEGHFP
                     VTRHMSVPVLDGDRVVLVAGVGNKAEPYDESDALQLELLMDGMWKVVQRTRSDERIRA
                     SLREKEVLLKEVHHRVKNNMQVIASMINLQFRDFEKTLARLTPGDISRDLQGRIRSMA
                     MIHEKLYQSGSFTSVNFGDYARNLGAALFRSYQMDETRVKLAIDAADVPLGVDAAIPC
                     GLILNELISNALKYAFPDGSRGAITVVLRPDEGGGYRLTVADDGVGLPDSVDIANIDT
                     LGLRLVVILAEQIEGTLSLSRDGGTACTIVFRRSGLDERGGTDSIR"
     gene            complement(2377..3060)
                     /locus_tag="PKJ16_16405"
     CDS             complement(2377..3060)
                     /locus_tag="PKJ16_16405"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HNV48627.1"
                     /translation="MKLIASWSGGKDSCLALHRALQAGHEIACLLNFVTEGEDRCAFH
                     GIPSRLLLLQAEAMGIPIVQTEMPRDMAGYESAFTRSVTNLAWSLDAGGMVFGDIYLE
                     EHREWVERVCGDIGIIALEPLWGGEPITISREFINAGFRTIVVSAKADLFTADDVGRD
                     YDMGFVETLVRRGVCPCGENGEFHTFVYDGPLFRNRIVINKTQKTLVDGFWEHWELDI
                     QGFSLSNND"
     gene            complement(3183..>4795)
                     /locus_tag="PKJ16_16410"
     CDS             complement(3183..>4795)
                     /locus_tag="PKJ16_16410"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HNV48628.1"
                     /translation="KGPLYAGVKGEAGSFNYLKGAGWAGGMISDWASFHVFGSRMAMG
                     DYRTDDYGTVHNSWQRDWHTGGNLTFYIGQKQTLRFGFSYSDMTSGFPEWRYTRGVYD
                     KEKYDHYVQDETFVYLEYDQNNRQYNHRERAHADAEYNLKALNDKIQWRSMVYYLRDF
                     NKTNYGDIYLNQKPEDYAYSSDDQTWGTDQQFTFVVPYNKIVVGYTGEHLKRITRNKE
                     AGKSATPFVPSMESRTNSGYAEDMVSLFDERLIFVIGGRYDSYEVNLLKPATGSYASF
                     SRTKRTFQNFSPRGGVVVRPLEWFRLRGNIGASFRPPTPEELAAEYELAWDRWLGNKD
                     LKPESGYTRETGFDISLRSFTFGATYFFTYAKNMIQKSGDKTEYQGKQWTTWENVGAV
                     EIEGYDVFASIAAGKLLELPVDLDLSSSATFNRRYRNMITNADLMFVADQEVKSSLRV
                     GYKQASVTLFHVYVGTQNVYNWDDPYNSCVEIKDPFYYFDLTFQWHISKHVKIDGGIF
                     NLTNRDYEWVRTYPMPERNYKLGVTGTL"
BASE COUNT          970 a         1504 c         1359 g          962 t
ORIGIN      
        1 ggagctcttc gagatggacg tgcacgccct gcgcgccccg gagagcgcca ccgggcttga
       61 ggaccaagtc gccgcggtaa cacgcgacgg cggcagcgta tacgagaccg tccaccgccg
      121 caaggacggc tcaaccttcc ccgtcgaggt gagcgcgcgc ctcatcgagg tactcgagaa
      181 acgattttat caggcgatca ttcgcgacat ctccgagcgc aagcggatcg agaacgcgct
      241 ctgggagagc caacgcatgt acgctacgct cgtgtccagc ctgccgggct tcgtctaccg
      301 ctgccggaac gaccgcaaat ggaccatggc cttcatcagc gacggctgct acgccatcac
      361 cggctatacg ccggaagact tcatcgacaa ccggacgatc gccttcaacg atatcgtcga
      421 gcacgagcgc cgcgacgagc tctggagcaa atggcaggac gcgcttgcgg cgcgcgagga
      481 gttcgaggat tcctatcgca tcatcactaa aagcggccta taccgatggg tgtgggaacg
      541 ggggcggggc gtgttcgcgg actcgggcga gctgttattc ctggagggct ttatcactga
      601 tgtaaccgcg ttgaaggaat ccgaatcgaa cctccagaag gcgaccgagc gcttccaaac
      661 ggccttcgag tatgcgccaa ccggcatgac cataaccggg acggatgggc gtttcatcga
      721 ttgcaatacc gccttctgcg caatgcttgg ctatacgcgg gaggagctcg ccggcatccc
      781 gttcaacgac atcacccatc ccgacgatcg cgacatcagc agggagcagc ttcttcgggt
      841 gatcgcgggg acgatcgatt ccgcaatatt cgagaaacgc tatctgcaca agagcggtgc
      901 cgtcatatgg gtgctcctga gcgccgcact ggtgaaaacg ccggacccgc aggacagcta
      961 ctgcattgcg catatgatcg acatcaccca gcgcaagcgg gcggaggatc agctccggct
     1021 cgacgagatg cgctttagcg cgctctacga acttgggcga atgcagaaca cgagcgaaaa
     1081 ggacattatc gatttcgcgc tcgaggaggg cgtgcggctc accgggagcg aggtgggtta
     1141 cgcgcacttc atcgaggacg accggatcag cctggagctc ttcacctggt cgcgcgcggc
     1201 cctggagcgc tgcaccgcgg agaagatgcg acactacccc gtcgagtccg cgggcatctg
     1261 ggcggactcc ctgcgcacca aacgggtcgt cttccacaac gactacccga accatccgga
     1321 ccgcaagggc tatcccgagg ggcacttccc cgttacgcgc cacatgagcg tgcccgtcct
     1381 cgatggcgac cgggtggtcc tggtcgccgg cgtgggcaac aaggcggagc cgtacgacga
     1441 atccgacgcg ctccagctgg agctcctcat ggacggcatg tggaaggtgg tgcagcgcac
     1501 gcgctcggac gagcggatcc gcgcctcgct ccgcgagaag gaggtcctcc tcaaggaggt
     1561 gcatcaccgc gtcaagaaca acatgcaggt gatcgcgagc atgatcaacc tgcagttccg
     1621 cgatttcgag aaaaccctcg cgcgcctcac cccgggcgac atcagccgcg acctgcaggg
     1681 acgcatccgc tccatggcga tgatccacga gaagctctac cagtcgggga gtttcacctc
     1741 ggtgaacttc ggcgactacg cccgcaacct gggcgccgcg ctcttccgct cataccagat
     1801 ggacgaaacg cgcgttaaac tcgccatcga cgccgccgac gtgccgctgg gtgtcgatgc
     1861 ggccatcccg tgcgggctca tcctcaacga gctgatctcc aatgccctca agtacgcctt
     1921 tcccgacggc agccgcggcg caatcaccgt ggtccttcgg cccgatgagg gcggcggcta
     1981 tcgcctcacc gtcgccgacg atggcgttgg cctgcccgac tccgtcgata tcgcaaatat
     2041 cgacaccctg ggattgcgcc tggtagtcat cctcgccgag caaatcgagg ggacgctctc
     2101 gctctcccgc gacggcggta cggcctgcac catcgtcttc aggcgctccg gcctcgacga
     2161 gcgcggcggc accgactcga tccgttaacg cattcttttg gctaccctgg tctttcgtgg
     2221 acacactaga aatgggggta caagggggct ctgacccctt tttttattcg atagtgcttc
     2281 catcctgtta tccgagcttc atttgagctc gggaaaaaga tggagcatag tgtcgcttcc
     2341 catttaatgg gctctgaccc ccatgcttct taacactcaa tcattattgc tcaaagaaaa
     2401 gccttgaata tcaagttccc agtgctccca gaatccatcg accagcgttt tttgtgtttt
     2461 gttgattaca attctattac ggaaaagcgg tccatcatat acaaaagtat ggaattcgcc
     2521 gttttctccg cagggacaga ccccacgacg gacaagcgtt tcgacgaatc ccatatcata
     2581 gtcgcggccc acatcgtccg ccgtgaagag atcggccttc gcgctcacca cgatcgtccg
     2641 gaaacccgcg ttgatgaact cccgggagat agttatcggt tcgccgcccc agagcggttc
     2701 gagcgcgatg atgccgatgt caccgcacac gcgctccacc cattcccggt gctcttcgag
     2761 gtagatgtcg ccaaacacca tccccccggc gtcgaggctc caggcgagat tcgtcacgct
     2821 tcgcgtgaag gccgattcgt atccggccat gtcccgcggc atctccgtct ggacgatagg
     2881 gatccccatc gcctccgcct gcaggagcag gagccgcgac gggataccgt gaaatgcgca
     2941 gcggtcctcc ccctcggtca cgaagttgag caggcaggcg atttcgtgcc cggcttgaag
     3001 ggcgcgatgc agcgccaggc agctgtcctt gccgccgctc catgatgcga tgagtttcat
     3061 tgaccctcct accccctccc gcgccgagca ttttttggac atcctgtcca tgctcggcat
     3121 tgcgacatcc tattgggaac ctcccccttc gttaggggga ggtggtgaaa tagaacatag
     3181 tgctatagag tccccgtgac gccgagcttg tagttgcgct cgggcatggg atacgtgcgc
     3241 acccactcgt aatcgcgatt cgtgagatta aagatgccgc cgtcgatctt cacatgcttg
     3301 ctgatgtgcc actggaaggt gagatcgaag taataaaacg gatccttgat ctcgacgcac
     3361 gagttgtagg ggtcatccca gttgtacacg ttctgcgttc ccacatacac atggaagagc
     3421 gtgaccgacg cctgcttgta tcccacgcgc aggctggact tcacctcctg gtcggcgaca
     3481 aacatgagat cggcgttcgt gatcatgttt cgatagcggc ggttgaaggt cgcgctcgac
     3541 gacagatcga gatcgacggg aagctccagc agttttcccg cagcgatcga ggcgaagacg
     3601 tcgtagccct cgatctcaac cgcccccacg ttctcccacg tggtccactg ttttccctga
     3661 tactcggttt tatcccccga cttctgaatc atgttcttcg cataggtgaa gaaatacgtc
     3721 gccccgaagg taaacgaccg gaggctgatg tcgaatcccg tctcgcgcgt gtagcccgac
     3781 tcgggcttga ggtctttgtt gcccaaccac cggtcccatg cgagttcgta ctcggcggcc
     3841 agctcctcgg gcgtgggagg acggaacgat gcgccaatat tgccacgcaa ccgaaaccac
     3901 tccaacggcc gcaccaccac gcctccccgc ggtgaaaaat tctggaaggt gcgctttgtc
     3961 ctgctaaacg aagcataact gccggtcgcc ggtttgagca ggttcacttc gtaggaatca
     4021 tagcgaccgc cgataacgaa gatcaatcgc tcgtcgaaca gcgacaccat gtcctccgca
     4081 tagccgctgt tagtacggga ctccatgctc gggacaaagg gcgtggccga ctttccggcc
     4141 tccttattgc gggtgatccg cttcaggtgc tcgccggtat agcccacgac gatcttgtta
     4201 tacggcacca cgaacgtgaa ctgctggtcg gtcccccagg tctgatcgtc ggagctgtag
     4261 gcgtagtcct ccggcttctg gttaagatag atgtcgccat agttcgtttt attgaaatcg
     4321 cgcagataat acaccatcga ccgccactgg atcttgtcat tcagcgcctt gaggttgtat
     4381 tccgcatcgg cgtgggcacg ctcacggtga ttgtattggc ggttgttctg atcgtattcc
     4441 agatacacga acgtttcatc ctggacgtag tgatcatact tctccttgtc gtacaccccg
     4501 cgggtatacc gccattcggg gaaaccgctg gtcatgtcgg aataggaaaa tccaaagcgc
     4561 agggtctgct tctggccgat gtagaaggtc aggttgccgc ccgtatgcca gtcgcgctgc
     4621 caggaattgt gcaccgtgcc gtaatcgtcc gtgcgatagt cccccatggc catccggctc
     4681 ccaaacacat gaaagctggc ccaatccgag atcatgccgc cggcccagcc ggcccccttc
     4741 agatagttaa agctccccgc ctcgcccttc acgcccgcat agagcggtcc cttcc
//
LOCUS       DAORHF010000271         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_18718_length_4780_cov_5.932487, whole genome shotgun sequence.
ACCESSION   DAORHF010000271 DAORHF010000000
VERSION     DAORHF010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_4780_cov_5.932487"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PKJ16_16415"
     CDS             complement(<1..308)
                     /locus_tag="PKJ16_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HNV48629.1"
                     /translation="MALNKLVDSRDVRFVLFEMLEVDKLNKYAKYADMDRDTLEATLD
                     LAEQIAMEQVYPANAPGDKEGSKYLPDTKGVKVPEVYKPAMKAYNEAGFIALATETEW
                     G"
     gene            complement(432..1148)
                     /locus_tag="PKJ16_16420"
     CDS             complement(432..1148)
                     /locus_tag="PKJ16_16420"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HNV48630.1"
                     /translation="MEKPLLFEVENGVAVITLNRPERRNAFNQALLAAIYDAIDEVSR
                     NGDIQAAVVTGAGTAFCSGIDLAVIGTDNLFDPRGDGSDMPDVMGACAKPIIGAVNGP
                     AITGGFELALNCDFLIASEQAQFADTHARVGIHPGWGMTQLLQQAVGVRMARQMSFAC
                     QRIDAQTALRIGLVNEVLPPERLLPRAKEIAAQICEANREMLSTIKSLIEYRHATAHD
                     AAYAHERAEFRAFVARVFAK"
     gene            1345..1884
                     /locus_tag="PKJ16_16425"
     CDS             1345..1884
                     /locus_tag="PKJ16_16425"
                     /inference="COORDINATES: protein motif:HMM:NF025116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HNV48631.1"
                     /translation="MRSYLVFSSMGPDRPGLADAIAEFFTARGINIERSRGSVLGNEF
                     GMIILTSGKTDSIDSLIGDLDSLRASTGLDIHVRRTKAPAQRESQPSIPYRLVATSID
                     HPGIVHQICKVLRERAINIDDMESNVDYNPFTGANIFHMICHFSLPPTVKIVDLRSDL
                     LRISDQFNIDIRFDAVVSK"
     gene            1881..3530
                     /locus_tag="PKJ16_16430"
     CDS             1881..3530
                     /locus_tag="PKJ16_16430"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HNV48632.1"
                     /translation="MKPPALAPIVDIAARAGIREDELEPYGRYKAKVRLEILDRVKRR
                     DDENLILVSAMTPTPAGEGKTTVSIGLAQAIAKLGKNVIAALREPSLGPVFGIKGGAT
                     GGGMSRVHPADEINLHFTNDFPAIESAHNLLAALIDNFVFHGNPYNIDPRRVAWRRVL
                     DMNDRSLRNIVIGLGGTANGFPRESGFDIVPSSEIMAILCLSRSYRELKEKIGNILVG
                     FTYENKPVFARDLKIEGAVTALLKHALLPNLVQTSEGVPVILHGGPFANIAQGANSIM
                     ATDLALRLADYVVTEAGFGFDLGAEKFFDIVAPYGGFSPRIVVLVATIRALKFHGGVD
                     RSELATPDPRAVIRGLPNLRTHYNNIAKFGAPCVIALNVFDGDSPEEIRTLVEAAENE
                     GMNIAPADIFRRGGEGGLELARKVVEVMATNTGRSQPLYRWELPVEDKIFKVASEIYG
                     AVSIDYQPQAKRDLELIKKNGFDKLPVCIAKTQQSLSDNPSLLGLPKDFVVTVREIKI
                     ASGAGFIIPITGEIMRMPGLAKVPAAFNIDIDDEGNITGIV"
     gene            3619..3900
                     /locus_tag="PKJ16_16435"
     CDS             3619..3900
                     /locus_tag="PKJ16_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HNV48633.1"
                     /translation="MFTVNEYFDGKVKSLGFTMADGPATIGVMAPGEYEFGTSSREIM
                     TVTSGSLTVKLPGSDEWRRFGENESFTVEAGRKFQLKVAADASYLCRYR"
     gene            complement(3982..>4780)
                     /locus_tag="PKJ16_16440"
     CDS             complement(3982..>4780)
                     /locus_tag="PKJ16_16440"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HNV48634.1"
                     /translation="NRTSPFAFTGNKFEFRAVGSSQSISFPATVLNTIVAESLDCVAD
                     AIAAKGGSPAQAAMDVIREELTKIKSVLFMGDNYTKEWEVEAGKRGLPNLKTTPEALS
                     GMTTDKARGLFKKYRVLSEVELMSRYHIRVERYVKELDIEAMTLANMVSSQIIPAAIG
                     YQGVLAASVTGAAGVLGKDADLAPQKELLKEVATLIGRMQSGVRDLVAKREKAHSFED
                     EHKKAEFFCSEVKAVMMSLREDVDAIEPIIDDARWPIPKLWEMLFIG"
BASE COUNT          884 a         1582 c         1429 g          885 t
ORIGIN      
        1 ccccattcgg tctcggtggc gagcgcgata aaaccggcct cattgtaggc cttcattgcg
       61 ggcttgtaga cctcgggcac cttcacgccc ttggtgtcgg gaagatactt gctgccctcc
      121 ttgtcgccgg gggcgtttgc gggatacacc tgctccatgg cgatctgctc ggccaggtcg
      181 agcgtcgctt cgagggtgtc ccggtccatg tcggcgtact tggcgtactt attgagcttg
      241 tcgacctcga gcatttcgaa gagcacgaag cgaacgtccc tcgaatcgac caatttattt
      301 aaagccattg ctgcctcctg aagtatttta aataaggtga ttaaaactat acggtgtata
      361 tagaactgaa agacaatgtt actacatgcg tctgtttaac taactaaaat ctgacttttt
      421 tttgtcaaca gctatttcgc aaacaccctc gcgacgaacg cgcggaactc ggcccgctcg
      481 tgcgcatacg ccgcgtcatg ggcggtcgcg tgccggtatt cgatgaggct tttaatcgtg
      541 gaaagcatct cccggttcgc ctcgcagatc tgtgcggcga tctctttcgc ccgcggcagg
      601 agccgttcgg gcggaaggac ctcgttcacc aggccgatcc ggagcgccgt ctgcgcgtcg
      661 atacgctggc aggcaaacga catctgccgg gccatgcgca cgcccaccgc ctgctgcagg
      721 agctgcgtca tgccccagcc gggatggatc ccgacccgcg cgtgcgtgtc ggcgaactgc
      781 gcctgctccg aggcgatgag gaagtcgcag ttgagcgcaa gctcgaaacc gccggtgatg
      841 gcgggcccgt tcaccgcgcc gatgatgggc ttcgcgcacg cccccatcac gtcgggcatg
      901 tcgctcccgt cgccgcgcgg gtcgaacaga ttgtcggtcc cgatgaccgc gagatcgatc
      961 cccgagcaaa aggccgtgcc ggcgccggtg acgacggccg cctgtatgtc gccattgcgc
     1021 gacacctcgt cgatcgcgtc gtagatcgcc gccagaagcg cctgattgaa ggcattgcgc
     1081 cgctcggggc ggttcagggt gatgacggcg acgccgtttt cgacctcaaa cagaagcggt
     1141 ttttccattg ctggctcctt gatggcgtga tggggtttta acagggtttt ccgtcgcgaa
     1201 atcgcgtcaa ggcaaaaaag ataaatgacg cctttgtctt gactttttcc ctgcgtccgg
     1261 cgacattcgc cgtcatcgcg cgcggggatc gcgcgcgaaa tcatactgaa cggaccggcg
     1321 acatctcgcc gcggggagga ctcgatgcgg tcatatctcg tattttcttc gatgggcccc
     1381 gaccggcccg gcctcgccga cgccatcgcg gaatttttca ccgcgcgcgg catcaacatc
     1441 gagcgctccc ggggaagcgt gctgggcaat gagttcggca tgatcatcct cacctcgggc
     1501 aagaccgatt cgatcgactc gctcatcggc gatctggatt cgctgcgcgc ctccaccggc
     1561 ctggacatcc acgtgcgacg caccaaggcc ccggcgcagc gggagtcgca gccgtccatc
     1621 ccctaccgcc tggtggccac gtctatcgac caccccggca tcgtccacca gatctgcaag
     1681 gtgctgcgcg agcgcgccat caacatcgac gacatggaga gcaacgtcga ctacaatccg
     1741 ttcaccggcg cgaacatctt ccacatgatc tgccacttct cgctgccgcc gacggtcaag
     1801 atcgtggacc tgcgctccga cctcctccgg atcagcgacc agttcaacat cgatatccgc
     1861 ttcgacgcgg tcgtgagcaa atgaaaccgc cggcgctcgc tcccatcgtc gacatcgccg
     1921 cccgggccgg catccgcgag gacgagctcg agccctacgg ccgctacaag gcgaaggtgc
     1981 gcctggagat cctggaccgc gtgaagcgcc gggacgacga gaacctcatc ctcgtatcgg
     2041 ccatgacgcc cacgcccgcc ggcgagggca agaccacggt ctccatcggc ctcgcgcagg
     2101 ccatcgccaa gctcgggaag aacgtgatcg ccgcgctgcg cgagccctcc ctggggcccg
     2161 tcttcggcat caagggcggc gcgaccggcg ggggcatgtc gcgcgtccat cccgccgacg
     2221 agatcaacct ccacttcacc aacgacttcc cggccatcga gagcgcgcac aatttgctgg
     2281 ccgcgctcat cgacaatttc gtgttccatg gcaacccgta caacatcgac ccgcgccgcg
     2341 tcgcctggcg ccgcgtgctg gacatgaacg accggtcgct gcgcaacatc gtcatcggcc
     2401 tcggcggtac ggccaacggc ttcccgcgcg agagcgggtt cgatatcgtt ccctccagcg
     2461 agatcatggc catcctgtgc ctttcgcgct cgtatcgcga gctcaaggaa aaaatcggaa
     2521 acattctggt ggggttcacc tacgagaaca aacccgtctt cgcgcgcgac ctaaagatcg
     2581 agggcgcggt gacggcgctg ctcaaacacg cgcttttgcc caacctggtg cagacctcag
     2641 agggcgtccc ggtgatcctc cacggcgggc cgttcgcgaa catcgcgcag ggcgcgaaca
     2701 gcatcatggc caccgacctg gcgctgcgcc tggcggacta cgtggtcacc gaggcgggct
     2761 tcgggttcga cctcggcgcg gagaagttct tcgacatcgt ggcgccctac ggcggctttt
     2821 ccccgcgcat cgtggtgctc gtcgcgacca ttcgcgcgct caagttccac ggcggcgtgg
     2881 accgcagcga gctcgcgacg ccagatccgc gcgcggtgat ccggggcctg cccaacctgc
     2941 gcacccacta caacaacatc gcgaagttcg gcgccccgtg cgtgatcgcg ctcaacgtct
     3001 tcgacggcga cagcccggag gagatccgga cgcttgtcga ggccgccgag aacgagggaa
     3061 tgaacatcgc cccggcggac atcttccgtc gcggcggcga gggcggcctc gagctcgccc
     3121 ggaaggtcgt cgaggtcatg gcgacgaaca ccggacggtc gcaaccgctg tatcgctggg
     3181 agctgccggt cgaggacaag atcttcaagg tggccagcga gatctacggc gcggtttcca
     3241 tcgactatca gccgcaggcc aagcgcgatc tcgagctcat caagaagaac ggcttcgaca
     3301 agctgcccgt ttgcatcgcc aaaacccagc agtcgctctc ggacaacccg agcctgctgg
     3361 gactgcccaa ggacttcgtg gtcacggtcc gcgagatcaa gatcgcctcc ggcgcgggct
     3421 tcatcatccc catcaccggc gagatcatgc ggatgccggg cctggcgaag gtgcccgccg
     3481 ccttcaacat cgacatcgac gacgagggaa atattacggg gatcgtataa gtagagaaag
     3541 ggcaattgtt tcagcaggga cagggcattg ccctgtccct actaattacc catcccctat
     3601 catacaaagg agcaatccat gttcacggtt aatgaatact tcgacggcaa agtgaaatcc
     3661 ctgggcttca ccatggcgga cggtccggcg accatcgggg tgatggcgcc cggcgaatac
     3721 gaattcggca cctcgtcgcg cgagatcatg accgtcacca gcgggagtct caccgtcaag
     3781 ctccccggca gcgacgagtg gcggcgattc ggagagaacg aatcgttcac cgtcgaggcc
     3841 ggccggaagt tccagctgaa ggtcgccgcc gacgcatcat acctgtgccg ctatcgctga
     3901 tccgaggcat cgcataggac atgatgaaaa cgcccccggc aagccggggg cgtttctcgt
     3961 tggcgactgt tcgatgcccg actacccgat gaacagcatc tcccatagtt tggggatggg
     4021 ccagcgcgcg tcgtcgatga tcggctcgat ggcgtccacg tcctcccgga gcgacatcat
     4081 cacggccttc acctcggagc agaagaactc ggccttcttg tgctcatcct cgaaggagtg
     4141 cgccttctcg cgcttggcga ccagatcgcg caccccgctt tgcatccgac cgatgagggt
     4201 cgccacctct ttgagcagct ccttctgcgg cgcgagatcg gcgtccttcc cgagcacgcc
     4261 cgccgctccg gtcaccgacg cggccagcac gccctgatag ccgatcgccg ccgggattat
     4321 ctgcgaggag accatgttcg ccagcgtcat cgcctcgata tcgagctcct tgacgtagcg
     4381 ctcgacccga atgtgatagc gggacatgag ctcgacctcg ctgagcaccc ggtacttctt
     4441 gaacagcccg cgcgccttgt cggttgtcat cccgctcagc gcctcggggg tcgttttcag
     4501 gttcggaagg ccccgcttcc cggcctcgac ctcccactcc ttcgtatagt tgtcgcccat
     4561 gaaaaggacc gacttgatct tcgtgagctc ctcgcggatc acgtccattg cggcctgcgc
     4621 ggggctcccg cccttcgcgg cgatggcgtc ggcaacgcaa tccaggctct cggcgacgat
     4681 cgtattgagc acggtcgccg ggaacgagat ggactgcgac gatcccaccg cgcggaactc
     4741 gaacttgttg ccggtgaagg cgaacggcga ggtccggttg
//
LOCUS       DAORHF010000272         4694 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19247_length_4694_cov_4.854063, whole genome shotgun sequence.
ACCESSION   DAORHF010000272 DAORHF010000000
VERSION     DAORHF010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19247_length_4694_cov_4.854063"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PKJ16_16445"
     CDS             <1..622
                     /locus_tag="PKJ16_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV48635.1"
                     /translation="VQDGDWITRGEKIDDGPVDPHKILDVKGHKDLQKFLVDEIQEVY
                     RLQGVGINDKHIEIIVRQMLRKVKITDPGDTTFVVEQTVDRFNFIDENARVEEEGGKP
                     ASAKPVLMGITKAALNTESFISAASFQETTRVLTDAAIKGKVDELRGLKENVIIGHLI
                     PAGTGYRDYRTIHVYQQEPGDLDFVPEEPTPEEKDEKEEKEPTAKE"
     gene            736..1110
                     /gene="rpsL"
                     /locus_tag="PKJ16_16450"
     CDS             736..1110
                     /gene="rpsL"
                     /locus_tag="PKJ16_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012538144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HNV48636.1"
                     /translation="MPTINQLIRKGRGQKRKKTKSPALRACPQKRGVCTRVSTVTPKK
                     PNSALRKVARVRLTNGIEVTAYIPGVGHNLQEHSAVLVRGGRVKDLPGVRYHIVRGAL
                     DTLGVDGRRKSRSKYGSKKPKA"
     gene            1175..1645
                     /gene="rpsG"
                     /locus_tag="PKJ16_16455"
     CDS             1175..1645
                     /gene="rpsG"
                     /locus_tag="PKJ16_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HNV48637.1"
                     /translation="MSRRRRPPKREILPDPKFGSKLITKFVNCMMMKGKKGVSETIFY
                     RAMDIIQEKTKKDPVEVFVQALENVKPVVEVKSRRVGGATYQVPVEIRSERRQALGIR
                     WIISYARGRSEKTMYQRLSGELMDAYNNTGTSIKKKEDTHKMAEANKAFAHYKW"
     gene            1910..3997
                     /gene="fusA"
                     /locus_tag="PKJ16_16460"
     CDS             1910..3997
                     /gene="fusA"
                     /locus_tag="PKJ16_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV48638.1"
                     /translation="MPRELPITRTRNIGIMAHIDAGKTTLTERILFYSGKTYKIGEVH
                     EGTAEMDWMAQEKERGITITAAATSVSWRNTRINIIDTPGHVDFTVEVERSLRVLDSA
                     IAVFDGVAGVEPQTETVWRQADKYAIPRICFVNKLDRIGADFARCVDMIRTKLFAEPL
                     PLQIPIGVEDAYRGVIDLVAMKGMVWGDDEGRTVEEIEIPSELADAARAHRESMIERL
                     SDFDDALMHKFLEGAEIGADEIKAAVRAATLSATLFPVFCGSALKNKGVQPLLEAIVD
                     YLPSPKDAKPVQGHSIENEDEMVSRDASDKEPFCALVFKIMSDPYVGKLAFARVYAGH
                     FKSGDQVLNVAKNKKERIGRILRMHANDREDIGEAYTGDIVAVVGLKLSATGDTLCAP
                     EHPLLLERMEFPNPVISVAIEPKTKADQEKLISALKRLEDEDPTFRVKLDPDTGQNLI
                     SGMGELHLEIIVDRLLREFAVKANVGKPQVAYKETIIKRVEADHVYEKEIGGALQYGH
                     VRISMEPAKPGSGLVFENALRDGAIPEEVVPFIVEGLKDGMASGVIAGYEMVDVKVAL
                     TGGSYRENDSVDVAYRIAANMALKDGARRADASLLEPVMKLEVVVPDEYTGDVINDLN
                     SRRGKMERLDMRAGLKIIDAHVPLSEVFGYATAVRSLTQGRASYTLQFSHYDVVPRNV
                     MEGIVARITGRSY"
     gene            4280..>4694
                     /locus_tag="PKJ16_16465"
     CDS             4280..>4694
                     /locus_tag="PKJ16_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HNV48639.1"
                     /translation="MGKEKFERTKPHVNVGTIGHIDHGKTTLTSAITKCLTAAYGGKN
                     KAIDFDQIDNAPEEKTRGITIATSHQEYETPNRHYAHVDCPGHADYIKNMITGAAQMD
                     AAILVVAATDGPMPQTKEHVLLARQVNVPNIVVFLN"
BASE COUNT         1083 a         1332 c         1464 g          815 t
ORIGIN      
        1 cgtgcaggac ggcgactgga tcacccgcgg cgagaagatc gacgacggcc cggtggatcc
       61 gcacaagatc ctcgacgtca aggggcacaa ggacctgcag aagttcctag tggacgagat
      121 ccaggaggtg taccggctgc agggcgtggg catcaacgac aagcacatcg agatcatcgt
      181 gcggcagatg ctgcgcaagg tgaagatcac cgacccgggc gacacgacct tcgtggtcga
      241 gcagacggtc gaccgcttca acttcatcga cgagaacgcg cgcgtcgaag aggagggcgg
      301 aaagcccgcg agcgccaagc cggtcctcat ggggatcacc aaggcggcgc tcaacaccga
      361 gtcgttcatc tcagccgcct cgttccagga gaccacccgc gttctcaccg atgcggcgat
      421 caagggcaag gtggacgagc tgcggggcct caaggaaaac gtgatcatcg gtcatctgat
      481 cccggcggga accgggtatc gggattaccg gaccatccac gtctaccagc aggagccggg
      541 cgacctcgat ttcgtacccg aggagccgac cccggaggag aaggacgaga aagaggagaa
      601 ggagcccacc gccaaggagt aaaatccttg acaaaaaggg tgcccaaaaa aatggtatgt
      661 aagcttttta cgcactgcca tgaataatcg gcggctcacg gcggagccgc gggagcgata
      721 taacgaggaa tacgaatgcc aacaatcaat caactgattc gcaagggtag aggacaaaaa
      781 cggaaaaaga cgaagtcgcc cgcattgcgc gcctgtcccc agaagcgcgg cgtatgcact
      841 cgggtgagca cggtcacgcc taaaaaaccg aactcggcgc tccggaaggt cgcgcgggtg
      901 cggctcacca acgggatcga ggtgacggcg tacatccccg gcgtgggcca caacctgcag
      961 gagcactcgg cggtgctggt acgcggcggc cgggtcaagg acctgccggg tgttcgctat
     1021 cacatcgtcc gcggcgcgct cgacacgctt ggggttgacg ggcggaggaa gagccgatcc
     1081 aagtacggat cgaaaaagcc gaaggcgtaa gcgcgccgtt tgaggcggcg ccatcatcta
     1141 acacgatacc acaccactgt aagaggaaac gtatatgtcg cgacgaagaa gacccccgaa
     1201 acgagagatc ctgcccgacc ctaaatttgg cagcaagctc atcaccaagt tcgtcaactg
     1261 catgatgatg aaaggcaaga agggcgtatc ggagaccatt ttctatcggg ccatggatat
     1321 cattcaggaa aagacgaaga aggacccggt cgaggtgttc gtgcaggcgc tggaaaacgt
     1381 gaagcccgtg gttgaggtga agtcccggcg ggtcggcggc gccacgtatc aggttccggt
     1441 ggagatccgc tcggagcggc gccaggcgct gggcatccgc tggatcatct cctatgcgcg
     1501 cggacgatcg gaaaagacca tgtatcagcg gctttcgggg gagctcatgg atgcgtataa
     1561 caacaccggg acctccatca agaagaagga agatacgcat aaaatggcag aggcgaacaa
     1621 ggcgtttgcg cattacaagt ggtaggatcc aataattgaa gcgaatgaaa aaataccagg
     1681 gccatgccct ggtatttttg tagccggaca taataaaaga cagagcggcg agtgcgcgtg
     1741 tctttgcaag gagcgcagga acggcgcaat cgacatgggt gttcgatggg gtttttcggc
     1801 gaggcggccg gatcttttcg cgtcacgtgc gatgataatg catcgagcgc cgttcgtgca
     1861 gttcactcgc catctctttt ccgtgccagg tgacttgtgg tagaacgata tgccccgtga
     1921 acttcccata acccgcaccc gcaatatcgg catcatggcg catatcgatg ccggcaagac
     1981 cacgctcacc gagcgcatcc tcttctattc ggggaaaacc tacaagatcg gcgaggtcca
     2041 tgagggcacg gccgagatgg actggatggc gcaggagaag gagcgcggca tcaccatcac
     2101 ggccgcggcg acctcggtgt cgtggcggaa cacgcggatc aacatcatcg atacgccggg
     2161 acatgtcgat ttcaccgtcg aggtggagcg cagccttcgc gtgctcgatt cggccatcgc
     2221 cgtgttcgac ggcgtcgccg gcgtggagcc gcaaacggag accgtctggc ggcaggccga
     2281 caagtacgcc atcccgcgca tctgcttcgt gaacaaactc gaccgtatcg gcgccgattt
     2341 cgcgcgctgc gtcgacatga tccgcaccaa gctcttcgcg gagccgctcc cgttgcagat
     2401 ccccatcggg gtcgaggacg cctatcgcgg cgtcatcgat cttgtcgcca tgaaggggat
     2461 ggtgtggggc gatgatgagg ggcgcaccgt cgaagagatc gagattccat ccgagcttgc
     2521 tgatgcggcg cgggcgcatc gcgaatccat gatcgagcgc ctgtccgact tcgatgatgc
     2581 gctcatgcac aaattcctcg agggcgccga gatcggggcc gacgagatca aggcggccgt
     2641 tcgggcggcg accctgagcg cgacgctctt ccccgtgttc tgcgggagcg cgctcaagaa
     2701 caagggcgtg cagccgctcc tggaggcgat tgtcgattac ctgccgtccc cgaaggacgc
     2761 caagccggtt caagggcaca gcatcgagaa tgaggacgag atggtgtcgc gcgatgcgag
     2821 cgacaaggag ccattctgcg ccctcgtctt caagatcatg tcggatccct acgtgggcaa
     2881 gctcgccttc gcgcgggtgt acgccgggca tttcaaaagc ggcgatcagg tcctcaatgt
     2941 ggccaagaac aagaaggagc gaatcgggcg gatcctgcgc atgcacgcca atgatcgcga
     3001 ggatatcggt gaggcctata ccggcgatat cgtggccgtg gtgggactca agctgtcggc
     3061 gaccggcgac accctctgcg cgcccgagca tccgctgctc ctcgagcgga tggaattccc
     3121 gaaccccgtc atctccgtgg cgatcgagcc gaagacgaag gcggaccagg agaagctcat
     3181 cagcgcgctc aagcgccttg aggacgagga tccgaccttt cgcgtcaagc tcgaccccga
     3241 caccgggcag aatctcatct ccggcatggg cgagctccac ctggagatca tcgtggaccg
     3301 cctgctgcgc gagttcgccg taaaggccaa tgtgggcaag ccccaggtgg cctataagga
     3361 gaccatcatc aagcgggtcg aggcagacca tgtctacgag aaagagatcg gcggtgcgct
     3421 ccagtacggg catgtgcgta ttagcatgga gcccgcaaag cccggaagcg gcctggtgtt
     3481 tgaaaatgca ctgcgcgacg gcgccattcc cgaggaggtc gttccgttca tcgtcgaggg
     3541 gctcaaggac ggcatggcct ccggcgtgat cgccgggtac gaaatggtcg acgtcaaggt
     3601 cgcgctcacc ggcggatcgt atcgggagaa cgattcagtc gatgtcgcct accgcatcgc
     3661 ggcgaacatg gcgctcaagg acggcgcgcg ccgggccgac gcgagcctgc tggaaccggt
     3721 gatgaagctc gaggtcgttg tgccggacga gtacaccgga gacgtcatca acgacctcaa
     3781 ttcgcggcgc ggcaagatgg agcggctgga catgcgcgcc gggctcaaga tcatcgacgc
     3841 gcacgtgccg ctctccgagg tcttcgggta cgcgaccgcc gtccgttcgc tgacgcaggg
     3901 gcgcgcctcg tacacgctcc agtttagcca ttacgatgtc gtgccgcgaa acgtgatgga
     3961 gggcattgtc gcgcggatca ccggccgctc ctattgacgc gcgaaacggc gcatgcgtca
     4021 taatgtcgtg tgaatatccc gggcgattat aattattttt tgctattttt tgttgacaga
     4081 aatgggtcat attttttaat tctcacactc taactgtcgg ctcagtaatg ttcggcttca
     4141 ttgtagcgtc ttaactgaca catcacacag aaggccgtaa acacgcttgc gacatttatc
     4201 aaccgtgacc gttgatgctc gaaacgcatc gatgtgttag cgacggtgtt attctgaaaa
     4261 aatttgtaag gagcaaaaga tgggaaagga gaaatttgaa agaacgaaac cgcacgtcaa
     4321 tgtgggcacc atcggccaca tcgaccacgg gaaaacgact ttaacttcgg caatcaccaa
     4381 gtgcctcacg gcagcgtatg gcggaaagaa taaggctatc gactttgacc aaatcgacaa
     4441 cgcgccggaa gagaagaccc gtggaataac gatcgcgacc tcgcaccagg aatacgagac
     4501 cccgaaccgt cactatgcgc acgtcgactg cccgggtcat gccgactata ttaagaacat
     4561 gatcaccggc gccgcgcaga tggacgccgc gattctcgtg gtcgccgcga ctgacggccc
     4621 catgcctcag acgaaggagc acgtgctgct cgctcgtcag gtgaacgtgc ccaatatcgt
     4681 cgtcttcctg aata
//
LOCUS       DAORHF010000273         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19288_length_4687_cov_4.736615, whole genome shotgun sequence.
ACCESSION   DAORHF010000273 DAORHF010000000
VERSION     DAORHF010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19288_length_4687_cov_4.736615"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2176)
                     /locus_tag="PKJ16_16470"
     CDS             complement(<1..2176)
                     /locus_tag="PKJ16_16470"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HNV48640.1"
                     /translation="MNLLAYLLLSLMAFLFNNRKRLKAALSHIDGYFNITVGFRSDDG
                     IVDRAISFAHGRARALPRVPETADVVLRFRDSRTALEMLRITPNETLMLILRNMMVLD
                     GDIAALQIFNYYVSLLMGRRHARMLARNRRRDERDRRRTYARTTAPLPERDRGAELMR
                     VPNSGAGVRFLDDPWLGTFTLEDFPRLRGMLDRHFSQMPSICVERPLIITRWFRENGF
                     ETDASGRPWNPELRQAMALRHLLASRRPIIARDDLIAGTTTAQEPTGVLIYPETHGGL
                     LWGELTSVSSRPLNPYRITPEDRELLHREIFPFWMHRNIKSHVRTKYGHPLCMRIDER
                     WVYYFAWKSVGISHTVPDYPRLLARGTQGIMDDIDRRLELIPASESSARDSLAAMRIA
                     LEGVNAYADNLSAEAARLAAVEPDPTRRAELEHISSICSRVPRRPAETLDEAVNAVWI
                     AWVALHLENINTGLSMGRLDQWLQPYFESDMERIPDEAGRAAYLRHAIELIGCLMMRG
                     TDHLPLVPDIGNYLFGGSSSDQAITLGGVTPAGGDGVNDMTYIFLKATELLAIRDPNV
                     NARVHPGITGDDYLRRLCHVNYLTAATPSLHNDAAVISSLSSNGYAEAHRRDWSATGC
                     VEPTISGRHMGHTGSVLMNMVAALEMALNDGGHPHMTERLGPETGDPARGAFPAFEDF
                     FRAYGTQQRFLIDQAVELNNSYAEAHAALRPIPLLSALLDGPI"
     gene            complement(2280..3521)
                     /locus_tag="PKJ16_16475"
     CDS             complement(2280..3521)
                     /locus_tag="PKJ16_16475"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV48641.1"
                     /translation="MKGHVDNGTIIRMHSGLITGRRHAELQRHIDDCDRCRKRAAAIA
                     ALLQQPPAGLRTPPERVRNRILQSYYEIKNAPAGASPINRMLHSWKAPALALAATIAI
                     VVGAAVFFDRWPFDRSGPATVAIDIAGLSGEVLVNGTMPEGTIRIKPHDTITVGKDGA
                     VRLAYAGIFTITLDAESEFIVEKASSPHTGAYEFAFRLNHGSLLSQFAHGDFSCAYSY
                     HTPRGTVSSTGTEFVLVAGPSETIVLLNEGAVRLTPSGRNEWIRVVERKRYAIARSIT
                     TSELGPGDLRAIDDMGAKRDSLAPANRESGDRNGRTRDTDMRKRGALPSDSGHRSVDG
                     GDNGFADGSEPVKPGSGKKAEGDAREPGAGDALREKHEADRDADRRKDLKEQGRDRRD
                     SLREKRRDKREQRGERERRNR"
     gene            complement(3545..4087)
                     /locus_tag="PKJ16_16480"
     CDS             complement(3545..4087)
                     /locus_tag="PKJ16_16480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48642.1"
                     /translation="MLIAEETVMELYDAYRDELYTYALRFVRTREMSEDILHDSFVRL
                     IEYSEHHEIRMGTIKPFLYRITHNLCVNALKKNRPLSAGGNGPEIADRSDVSKNHELD
                     ELNDTIDELVDTLDDESRSIFIMRKDLGMSFEEIAANLGISERTVRRKIAGALSFLAE
                     QLKKTGYIDSYLILLSILLG"
     gene            complement(4116..>4687)
                     /locus_tag="PKJ16_16485"
     CDS             complement(4116..>4687)
                     /locus_tag="PKJ16_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48643.1"
                     /translation="YDRKLEEYFFSTKVGYTNTNTDTYNGALAVNPGEHVSIEGSYQY
                     SNAFYVQFDDNYTKHLGRLGATILPMKELGLTLGGEYGQDSARYVLYGGDVGFTLKLF
                     GRLKFLASYTYLWYQAPLTTTAEKSNAKSSRPKPNQAALPASAITTNAGLSSATNAPQ
                     TGTNPYVRTSLINESYANHAVVFGVTLRI"
BASE COUNT          768 a         1385 c         1587 g          947 t
ORIGIN      
        1 cgatggggcc gtccagaagt gcgctcagga gcgggatggg gcgcagcgcc gcgtgcgcct
       61 cggcgtagct gttgttgagc tccaccgcct ggtctatcag aaagcgctgc tgagtcccat
      121 aggcgcggaa gaaatcctcg aaggcgggga aggcgccgcg cgcgggatcg cccgtttcgg
      181 ggcccagccg ctcggtcatg tgcgggtgcc cgccgtcgtt gagcgccatc tcgagcgcgg
      241 cgaccatgtt catgagcacc gagccggtgt gccccatgtg ccgcccggag atggtgggct
      301 ccacgcagcc cgtcgcggac cagtcgcgcc ggtgggcctc ggcgtagccg ttggacgata
      361 gcgacgagat gaccgccgcg tcgttgtgca gcgagggggt cgcggcggtg aggtagttca
      421 cgtggcagag acgccgcagg tagtcgtcgc cggtgatccc ggggtggacg cgcgcgttca
      481 cgttgggatc gcggatcgcc aggagctccg tcgccttgag gaagatgtag gtcatgtcgt
      541 tcacgccgtc gccgccggct ggggtcacgc ccccgagcgt gatcgcctgg tcggacgagc
      601 ttccgccgaa gaggtagttc ccgatatcgg ggaccagcgg caggtgatcg gtcccgcgca
      661 tcatcaggca gccgatgagc tcgatcgcgt ggcgcagata ggccgcgcgc ccggcctcgt
      721 ccgggatgcg ctccatgtcg ctttcgaagt acggttggag ccactggtcg agccgtccca
      781 tcgagaggcc ggtgttgatg ttttcgaggt gcaatgcgac ccacgcgatc cagacggcgt
      841 tcaccgcctc gtcgagggtt tcggcgggac ggcgcggaac gcgagagcag atcgacgaga
      901 tgtgctcgag ctctgcgcga cgcgtcggat cgggctcgac ggcggccagg cgcgcggcct
      961 cggccgagag gttgtcggcg taggcgttca cgccctcaag cgcgatccgc atggcggcga
     1021 ggctgtcgcg tgcggaggat tccgatgcgg gaatcagctc caggcggcgg tcgatatcgt
     1081 ccatgatccc ctgcgttccc cgcgcgagca ggcgcggata atcgggaacg gtgtgcgaga
     1141 tccccacgga tttccaggcg aaatagtaca cccagcgctc gtcgatgcgc atgcagagcg
     1201 gatgaccgta cttcgtacgg acgtggctct tgatgttccg gtgcatccag aaggggaaga
     1261 tctcgcggtg gagcagctcg cggtcctcgg gcgttatgcg atatggattg agcggacggc
     1321 tggacaccga ggtgagctcg ccccagagga gcccgccgtg cgtctcggga tagatgagga
     1381 cgcccgtggg ctcctgggcc gtggtggtgc cggcgatgag gtcgtcgcgc gcgatgatgg
     1441 gccggcggct cgcgaggagg tgccgcagcg ccatcgcctg gcggagttcg ggattccacg
     1501 gccggccaga cgcgtcggtc tcgaaaccgt tctcgcggaa ccagcgcgtg atgatgagcg
     1561 ggcgctccac gcagatcgac ggcatttggg agaagtgccg gtccagcatg ccgcggaggc
     1621 gtgggaagtc ctcgagggtg aaggttccaa gccagggatc gtcgaggaag cgaacgcccg
     1681 ccccagaatt cgggacgcgc atgagctccg cgccccggtc gcgctcgggg agcggggccg
     1741 tcgtgcgcgc ataggttcgg cgacgatcgc gctcgtcgcg ccggcggttc cgggcgagca
     1801 tgcgcgcgtg gcggcgtccc atgagcagcg acacgtagta gttgaagatc tgcagcgcgg
     1861 cgatgtcgcc gtcgagcacc atcatgtttc gtaaaatgag catgagcgtc tcgttgggcg
     1921 ttatgcgcag catctccagc gccgtgcggc tgtcgcggaa gcgaagcacc acgtcggcgg
     1981 tctcgggaac gcgcgggagc gcccgggcgc gtccgtgtgc gaacgaaatg gcgcggtcga
     2041 cgatcccgtc gtccgaccgg aagccgacgg tgatgttgaa atatccgtca atgtggctga
     2101 gggcggcctt gagccgcttg cggttgttga acaggaacgc catcagagaa aggagcaggt
     2161 aggcgagcag gttcatggcg gtcctccagg gaggcggaat cggatgttac gcaggggtat
     2221 ggtacgggtt tgtttcagga aacgtcaaga tatttgcggg attcggccgg tttcccggat
     2281 caccggtttc tccgttcacg ctctccacgc tgctctcgct tgtcacggcg cttttcacgc
     2341 agggagtcgc gacgatccct gccctgctcc tttaggtctt tccggcggtc ggcgtctcga
     2401 tccgcttcgt gcttttcccg aagcgcgtcg cccgctcccg gctcccgtgc gtccccctcg
     2461 gcttttttgc cgctgcccgg ttttaccggc tcgcttccat cggcaaaacc attatcgcct
     2521 ccgtcgacgg aacgatgtcc ggaatcagag gggagggcgc cgcgcttccg catgtccgtg
     2581 tcccgtgtgc gcccgttgcg gtcgccggat tcccggttcg ccggggccag gctatcgcgt
     2641 ttcgcgccca tgtcgtcgat ggcacggagg tccccgggcc cgagctccga ggtggtgatc
     2701 gagcgcgcga tcgcgtaccg tttccgctcc acgacgcgga tccattcatt cctcccggat
     2761 ggggttagcc gcaccgcgcc ctcgttcagc aggacgatcg tctctgacgg tccggcgacg
     2821 agcacgaatt cggtcccggt ggagctcacc gttccgcgcg gggtgtgata ggagtacgcg
     2881 caggagaagt ctccgtgggc gaactgtgaa agcaggctcc catggttcag cctgaaggcg
     2941 aactcgtatg cgcccgtgtg tggcgatgaa gccttttcga cgatgaattc gctttccgca
     3001 tccagggtga tagtgaagat tcccgcgtat gcgagccgga cggcgccgtc cttccccacg
     3061 gtgatggtat cgtgcggttt gatgcggatc gttccctccg gcatcgtgcc gtttacgagg
     3121 acctcgcccg acaatccggc gatatcaatc gccaccgttg cggggcctga tcggtcgaat
     3181 ggccagcggt cgaagaacac cgccgcgccg acaacgatgg cgatcgttgc ggcgagcgcc
     3241 agcgcgggcg ccttccatga atgcaacatc cggtttatgg gcgatgcccc cgcaggtgcg
     3301 ttttttatct cgtaatacga ctgcaggatt cggttgcgca cccgttccgg tggggtgcgg
     3361 agaccggcgg ggggctgctg cagaagggcg gcgatggcgg cggcgcgctt tcggcaccgg
     3421 tcgcagtcgt cgatgtgccg ctgcaactcc gcatgccgcc tgccggtgat gagaccggag
     3481 tgcatcctga tgatggtgcc gttgtctaca tgccctttca tgacgttccc attaacagta
     3541 caaatcaccc cagcaaaatt gacaaaagta taagataaga atcgatgtac ccggtttttt
     3601 tcagctgttc ggccaggaac gagagcgcac cggcgatctt tcttctgacg gtccgttccg
     3661 aaatgcccag gttcgcggca atttcctcga aggacatgcc gaggtccttc ctcatgatga
     3721 agatcgaccg tgattcatcg tccagcgtgt cgacgagctc gtcgatcgta tcattcagtt
     3781 cgtcgagttc atggttcttc gatacgtcgg agcggtcggc gatctcaggg ccattgcctc
     3841 ccgccgatag cggtcggttc ttttttaggg cgttcacgca caggttgtgc gttatccgat
     3901 acaggaacgg tttgatggtt cccatgcgaa tctcgtggtg ttcggaatac tcgatgagcc
     3961 tcacgaagct gtcgtggagg atgtcctcgg acatctcgcg cgttcggaca aatcggagcg
     4021 cgtaggtgta gagctcgtca cgataggcgt catagagctc catgactgtt tcttccgcga
     4081 tcagcatgat tatcgcccct cggacccccg gtcaatcata tccgcagcgt tacgccgaag
     4141 acgaccgcgt ggttcgcgta cgattcgttg atcaacgagg tccgcacgta cggattcgtt
     4201 ccggtttgcg gagcgttggt cgccgaactc aatcccgcgt ttgtcgttat ggcggaggcg
     4261 ggcagtgccg cctggttggg ctttgggcgg ctcgacttcg cattagattt ctccgccgtc
     4321 gttgtcaagg gcgcttgata ccacaggtag gtgtaggatg ccaaaaattt cagccggccg
     4381 aagagtttga gagtgaagcc gacgtcgccg ccgtacagga cataccgtgc cgaatcctgt
     4441 ccgtactccc cgcccagtgt gaggcccagc tctttcatgg gcaggattgt cgcccccagc
     4501 cgccccaggt gtttggtgta attgtcgtcg aattggacat agaacgcgtt gctgtactgg
     4561 tagcttccct cgatgctcac atgctcgccg ggattgaccg ccagcgcgcc attgtaagtg
     4621 tcggtgttgg tgttcgtata gccgactttg gtgctgaaga agtattcctc gagcttgcga
     4681 tcatagc
//
LOCUS       DAORHF010000274         4687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19290_length_4687_cov_4.224525, whole genome shotgun sequence.
ACCESSION   DAORHF010000274 DAORHF010000000
VERSION     DAORHF010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19290_length_4687_cov_4.224525"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PKJ16_16490"
     CDS             complement(<1..192)
                     /locus_tag="PKJ16_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HNV48644.1"
                     /translation="MIERFFRPFFAGVFFAPDLDVSSRSFEFVFHATAPHATRDWQLY
                     VTRDGWDPKQPLGWGDVDSF"
     gene            complement(308..610)
                     /locus_tag="PKJ16_16495"
     CDS             complement(308..610)
                     /locus_tag="PKJ16_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HNV48645.1"
                     /translation="MPQIPHETRAVDKPVTRDGDGKHVTDEYTTARNRFIRALVVLIL
                     AIVYTISPIDLIPEALVGPLGLADDLFLLLIGFLYAWYSYSKLKRQRALVPRPKPQ"
     gene            complement(689..1072)
                     /locus_tag="PKJ16_16500"
     CDS             complement(689..1072)
                     /locus_tag="PKJ16_16500"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV48646.1"
                     /translation="MTTEVKKLKTDAYHCNECVIVPAIDIYEDANEFVLTADMPGVAR
                     EDLEITMKDNTLTVDGKVSPAYLSAEDLKYREYTLYNFHRVFQVDDAVNAGGITAKLD
                     NGVLVITLPKSEKAKPRKIEITVEK"
     gene            complement(1090..1500)
                     /locus_tag="PKJ16_16505"
     CDS             complement(1090..1500)
                     /locus_tag="PKJ16_16505"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV48647.1"
                     /translation="MYTNYDIFDDMLGLRNTIDGYFSNLAPRERAIDYPHINLYEKDN
                     AITIHALMPGVANDAISIQLVDNSLIIEGERKPDYADKPYIRRERSFGKFKKSIRLPY
                     AVNRETVTAARRDGVLIITLQKSPEALPRKIEIK"
     gene            complement(1561..2019)
                     /locus_tag="PKJ16_16510"
     CDS             complement(1561..2019)
                     /locus_tag="PKJ16_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HNV48648.1"
                     /translation="MKWGLSKRENKQNQGIDSFRREIQNLFDDFFTVGPSSLFDAAWA
                     PSIDIEESDTAIHIKAEVPGIEEKNLSVSIENNMLIISGEKNEERKEEGTDKRYVVSE
                     RRFGSFHRSIQLPENIKSDKISAKFKNGVLAIDIPKDETAQPKKITIDVH"
     gene            2227..2430
                     /locus_tag="PKJ16_16515"
     CDS             2227..2430
                     /locus_tag="PKJ16_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48649.1"
                     /translation="MDISGIQQSISGQRLYQKGSIETTNEYMKSSSALGSEGGNGQAS
                     VQKSVISTANEFTRKGTVLDTTA"
     gene            complement(2420..2866)
                     /locus_tag="PKJ16_16520"
     CDS             complement(2420..2866)
                     /locus_tag="PKJ16_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HNV48650.1"
                     /translation="MESINVLLIFKQQIMYKPIIFRLATEYNVVFNVLEAKILPKQEG
                     RLILELSGGRRELDDCIDYLKREEVQVEVLADKIYRDERACVHCGACTAVCRVDALTI
                     DRVTMEVRFYPERCVACGLCKTACPVKAMSGISIDFEFDGDVKATP"
     gene            complement(2904..3350)
                     /locus_tag="PKJ16_16525"
     CDS             complement(2904..3350)
                     /locus_tag="PKJ16_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48651.1"
                     /translation="MIGSPEGAQEAHRSITLTKIIVRNAALARYRQHRRTIFAGQIPD
                     CRCERVPIDTHRQIEYNDIGTFRFQSRDEIVRCSGWRHDPIAQSGKQICKLRIDGFSE
                     FRNQYRVFVVHISTTVPTCRYACMGILARQKSIDGKNPPELILFLR"
     gene            3741..>4687
                     /locus_tag="PKJ16_16530"
     CDS             3741..>4687
                     /locus_tag="PKJ16_16530"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV48652.1"
                     /translation="MARDNTDTVAMLDNLEWNAEVNAVVAELYGMILQSSSIEELSDI
                     ILDHAKRLTDSVFGFVGYIDPYSGNLVSCTMTKDVWETCNIPDKRIVFEKFCGLWGWV
                     LENSQPMLCNDVSADPRSTGVPRGHIPIDRFLSVPSLFGQKLVGMIALANARDPYTQR
                     DLDLVSRLGSIYAIAVNHHFLRQDLIEAKEAAEYANRAKNEFLANVSHELRTPLNAIL
                     GFGQILEMQKEGRLSMPQREYLRHILKSGHYLLHLVNDILDVSAIEAGRLALVQRSFD
                     LGALIRDLLPEHRLLAEKKRIPILDDVAGDLNGFFADPVR"
BASE COUNT          910 a         1269 c         1398 g         1110 t
ORIGIN      
        1 gaacgagtcg acgtcgcccc agcccagcgg ctgcttcggg tcccagccgt cgcgcgtcac
       61 gtagagctgc cagtcgcgcg tcgcgtgcgg cgcggtggcg tggaacacga actcgaagct
      121 gcggctcgac acatccagat ccggcgcgaa gaagaccccg gcgaagaagg gccggaagaa
      181 ccgctcgatc atccgggacg agaaccccag gtcccgcagc cgctgcatgc gatcgtttta
      241 aagagccgtt tccggcggtc ccgcttccgg ggggtctgtc gtgcgttcac gatgctcgcc
      301 cggtcgctta ctggggtttg ggccggggga ccagcgcgcg ctggcgcttg agtttcgaat
      361 agctgtacca ggcgtagaga aagccgatca ggaggagaaa caggtcgtcg gcgagtccca
      421 gcgggcccac gagggcctcg gggatgaggt cgatgggcga gatggtgtag acgatggcca
      481 aaatgagcac cacgagggcg cgtatgaatc gattgcgggc ggtggtgtat tcgtccgtta
      541 catgttttcc gtcgccgtcc cgcgttacgg gcttgtcgac ggcgcgcgtt tcatggggaa
      601 tctgtggcat ggcgtgctcc tttccgggga atcccgccgg cggcccggcg ggggccgtcg
      661 catgacgacc cctttcgcac gggcggggtt acttttcaac cgttatttcg atttttcgcg
      721 gtttcgcctt ctcgctcttg ggtagggtga tgacgagaac accgttgtcg agtttcgcgg
      781 tgatcccgcc ggcgttcacg gcgtcatcga cctggaagac gcgatgaaag ttgtagaggg
      841 tatactcgcg gtatttcagg tcctccgcgc tcagataggc cggggaaacc tttccatcga
      901 ccgtgagggt gttgtccttc atggtgattt ccaggtcttc ccgcgcgacg cccggcatgt
      961 cggctgtgag aacgaactcg ttcgcatcct cgtagatgtc gatggcgggg acgatcacgc
     1021 attcattgca gtggtatgcg tcggttttca gttttttcac ctcggtggtc atgacggttc
     1081 ctcccttcgc tatttgatct cgatcttgcg gggcagcgcc tccgggctct tctgaagcgt
     1141 tatgataagc accccgtccc tgcgcgctgc cgtcacggtt tctcggttca cggcgtaggg
     1201 cagccggatt gatttcttga atttcccgaa gctgcgttcg cggcggatat acggcttgtc
     1261 ggcgtaatcg ggcttccgct caccttctat gatgaggctg ttgtcgacga gctggataga
     1321 aatcgcatcg ttcgcgaccc ctggcatgag cgcgtggatc gtgatggcat tgtccttctc
     1381 gtacaggttg atgtgcggat aatcgatcgc gcgttcccgc ggagcgaggt tcgagaaata
     1441 gccgtcgatg gtgtttcgca gtccgagcat gtcatcaaag atgtcgtagt tggtatacat
     1501 gggattcctc cttcgtgtga tgtcgcctct ccgcgtattg cgcggagagg cgtttcaggt
     1561 tcaatggacg tcgatcgtga tcttcttcgg ttgcgccgtc tcgtccttcg gtatgtcgat
     1621 ggcgagcacg ccgttcttga atttcgcgga aatcttgtcg ctcttgatgt tctcgggaag
     1681 ctgaatgctc cggtggaacg agccgaatcg ccgctcggag acgacatacc gcttgtccgt
     1741 gccttcctcc tttcgctcct cgtttttttc gccgctgatg atcagcatgt tgttctcgat
     1801 ggaaaccgac aggttcttct cctcaattcc ggggacctcg gccttgatgt gtatcgccgt
     1861 atcgctttcc tcgatgtcaa tcgagggggc ccaggctgcg tcaaagaggg atgacggccc
     1921 tacagtaaag aaatcatcga atagattctg aatttctctc ctgaagctgt cgattccttg
     1981 attttgttta ttttctcgtt ttgacaatcc ccatttcata acacacctcc gattgtgtta
     2041 ttttaactgc tagcactcta gtgaaacgag tgcttatttt ttgcacttaa aatataccta
     2101 tcaaaaagtt gaacgtcaat agggggatgc tcgttttttg aaaaaaaatg ttgggagtat
     2161 ttttgcttga attataaacc cattgttact attttattat cagcgcaatg caaccatgga
     2221 gggtgtatgg atattagcgg cattcaacag agcatatccg gtcagcgact ctatcagaaa
     2281 gggtccatcg aaacgaccaa tgagtacatg aagtcctcat cggcgttggg atcggagggg
     2341 ggcaatgggc aggcctccgt tcagaagagc gtgattagca cggccaacga gttcaccagg
     2401 aagggtacgg tgctcgacac tacggcgtag cttttacgtc tccgtcgaac tcgaaatcta
     2461 tcgagatgcc cgacatcgcc ttgacggggc aggcggtctt gcacaggccg caggcaacgc
     2521 agcgctcggg ataaaatcgg acctccatcg tcacgcggtc gattgtgagc gcgtcgacgc
     2581 ggcagacggc cgtacatgcg ccgcagtgta cgcacgcgcg ctcgtcccgg tagatcttgt
     2641 ccgcgagcac ctcaacctgg acctcttcgc gcttcaggta gtcgatgcag tcgtcgagct
     2701 cgcgtcgtcc tccggagagc tcaaggataa gcctgccctc ctgcttcggc aggatcttcg
     2761 cctcaaggac gttgaacacg acattgtatt cggtggccag ccggaagatg atcggtttat
     2821 acatgatctg ctgcttgaag atgagcagga cgttgatcga ttccatgggt ttctcctcga
     2881 gtacaggaat tggccgcggg gcgtcaacgc aagaataaaa tcagttccgg agggtttttt
     2941 ccgtcgatcg atttctgtct tgctagaata cccatgcagg cgtagcggca ggttgggacg
     3001 gtagtgctga tatgaacaac gaacacacga tactggttgc ggaattcgga gaaaccgtcg
     3061 attcgcagct tgcagatctg cttgccggat tgggctatcg gatcatggcg ccatccgctg
     3121 caacggacga tttcgtcgcg cgattggaag cggaacgtcc cgatatcgtt atactcaatc
     3181 tgtcggtggg tgtcgatggg gacgcgctcg catcggcaat ccgggatctg gcccgcaaag
     3241 atcgttcggc ggtgctgacg atatctcgcg agagcggcat ttcgcacgat gatcttggtg
     3301 agcgtgatcg agcggtgtgc ctcctgcgcc ccttcagggg atccgatcat cgggaggccg
     3361 tacgggacgc cctactgcgc gcgattaccc gtgaggcctc gcacgggatg cgtcgttttc
     3421 aggctcttgc cgaatcggcc ccgtatccaa tagttatatt cgatgcgggc gaacgaatcg
     3481 tttatgccaa ccgaaaatca tcagaattat ttcaagtgaa cgtggcggaa cttgccggaa
     3541 aagggatcga tgacttgttt ccccacgata cggttgacat agcgaaccgg ctcacggacg
     3601 ccttgcgcga ggccatgcgt acgggcgcct ggaccactgt gagcgattgt aaaatggaat
     3661 actccgacgg ggcgcgctgg ttcagtttca ttttcgcgcc gcttgacggc gatgcaggct
     3721 cagacaacgg ggcggtatgc atggcccgtg acaataccga caccgtggcc atgctggaca
     3781 acctcgaatg gaacgccgag gtgaacgcgg tcgtcgccga gctctacggc atgatcctcc
     3841 agtcctcgtc gattgaggag ctgtccgaca tcattctcga tcacgcgaag cggctcaccg
     3901 acagtgtgtt cggtttcgtg ggatatatcg acccctattc cggaaatctc gtttcgtgca
     3961 ccatgaccaa agacgtctgg gagacgtgca acatccccga taagcggatc gtcttcgaga
     4021 agttttgcgg cctctggggg tgggtgctcg aaaacagcca gccgatgctc tgcaacgacg
     4081 tgtctgcgga tccgcgctcg accggggtcc cccgggggca tatccccatc gaccgctttc
     4141 tgtccgtgcc gtcgttgttc ggccaaaagc tcgtgggcat gatcgcgctc gccaacgcga
     4201 gagatcccta cacgcaacgc gatctcgatc ttgtatcccg gctcggttcg atttacgcca
     4261 tcgccgtcaa ccatcacttt ttgcggcagg acctcatcga ggcgaaggag gcggcggagt
     4321 atgcgaatcg agcgaagaac gagtttctcg ccaatgtgag ccatgagctg cggacgccgc
     4381 tcaacgccat cctgggattt ggccagatat tggagatgca gaaagaggga aggctttcga
     4441 tgccgcagcg tgagtatttg cgtcatatcc ttaaaagcgg gcattatttg cttcatctgg
     4501 taaacgacat cctcgatgta agcgccatcg aagcgggcag gttggcgctc gtgcaacggt
     4561 cgttcgatct cggggcgctg atccgcgacc tgcttcccga gcatcggctg cttgccgaga
     4621 aaaagcgaat ccccattctc gatgatgtgg ccggagacct gaacggcttt ttcgccgatc
     4681 cagtccg
//
LOCUS       DAORHF010000275         4647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19529_length_4647_cov_6.251089, whole genome shotgun sequence.
ACCESSION   DAORHF010000275 DAORHF010000000
VERSION     DAORHF010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19529_length_4647_cov_6.251089"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PKJ16_16535"
     CDS             <1..367
                     /locus_tag="PKJ16_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HNV48653.1"
                     /translation="LADPALGTPIAIAIAIHNIPEGIAVAVPIFYATGSRRKAFGYSF
                     LSGLSEPVGALLGFVLLRIYFSGAVMGASLAAVAGIMVFISLDELLPAAREYGQPHLA
                     IYGLVAGMAVMAMSLLMLH"
     gene            complement(384..1778)
                     /locus_tag="PKJ16_16540"
     CDS             complement(384..1778)
                     /locus_tag="PKJ16_16540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV48654.1"
                     /translation="MNQARSRSGFNLSRRLYGPRNLGFVLAALPLAALLYQHDAVAAC
                     WGALALTCFAWPHVAYWIARRADNSNRAELRNLQIDSVLGGAWCAAIGFNPLVGPVMF
                     SMLMMDAIAVGGSRLLARCLAAYAAAALAGGLVTGFRVDAGISLMTALACLPLMILFP
                     SINGTIAYLRTRQVVRLKNELERQKDALFDVQFRLEEANRDLRDTNDTLRQAQRIAAH
                     DMRLAANVQMSVLPGAEPSVEGWEIAFYFRPMSGVSGDFYDFYVRDGRLEGVALFDVS
                     GHGISSALITMLAKSLLYRLFVSGLAGPLGSVLTAFNHELFGDIGQSQYYLTGVLLRI
                     DGDRVEYVNAAHQEIVRRTARGVEPVRPETDEHPGGFFLGISSAPQEYDAVAFVVEAG
                     ELVLLYTDCLSEARNAEGELYGRERISASLLRASDTSAQAALSGLCEDFYSFVNERGT
                     LNDDLTIIVLRKTG"
     gene            1984..2643
                     /locus_tag="PKJ16_16545"
     CDS             1984..2643
                     /locus_tag="PKJ16_16545"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48655.1"
                     /translation="MTAIVGTKSERTRARIVARVAPLFNRKGYAGTSLSDMTEAAGLT
                     KGAIYGNFDGKDEVALHAFRHNIGGIIAELEGAVRSRDRCADKLRAYTETYRRIYPAI
                     AAGGGCPLANTLCDADDTHPGLRAEAGRMLARWRDTIARIVARGIASGECAPSADPRR
                     AAMTIITLIEGGALLAAGARSADYFMNALDRVDDYIDALCRHDDGGAPPQRYITTRRR
                     R"
     gene            2640..3701
                     /locus_tag="PKJ16_16550"
     CDS             2640..3701
                     /locus_tag="PKJ16_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV48656.1"
                     /translation="MSDIYRELQQRLDTYSVGFPATESGIEIRILKKLFTEEEARFFL
                     AMSPLLEAPASVAERTGMPVDAAAARLEDMASRGLLFRQKKGDSARYGAIPFVHGLIE
                     FKVKDLDPELAALFLQYSDEGFDRSFVNVKGAFLRTIPVRQSITPEHHVAALDDVEQI
                     LAGIDTIVVTDCICRKGKAMVSGSCGAPVEVCFMFGSMARYYLDNNMGRRVTAEEALA
                     LTRTAQEAGLVTQPSTSQNPNGMCNCCGDCCGILSSIKKTPRPADLVYTNHFSEVIAD
                     ECAGCEDCIAWCPMAAISMDADGTARIALERCIGCGVCVPKCPADAIVLRAKPEGERR
                     ALPKNALEQMMLMAQERTR"
     gene            complement(3705..4364)
                     /locus_tag="PKJ16_16555"
     CDS             complement(3705..4364)
                     /locus_tag="PKJ16_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015723892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HNV48657.1"
                     /translation="MGGDTLLTLVGIFGASFAVAFSGALMPGPLLAVTVREAAARGFW
                     AGPILIAGHAILECALVALLLVGLGALLATDAAFGVIGAAGALVMLWMAYGMFRALPE
                     LRISSGDGTSSGMHPVLAGIVMSVANPYWSIWWATIGLGYITYSMREGALGVASFFTG
                     HILADLAWYSFVAFGIARGRRFIGDRAYRALIGVCAVALVVFALWFGLAGARRLMALA
                     L"
BASE COUNT          911 a         1582 c         1449 g          705 t
ORIGIN      
        1 actcgccgat cccgcattgg ggacgcccat cgccatcgca atcgcaatcc acaacatccc
       61 cgaagggatc gccgtcgcgg tgcccatctt ctacgccacc ggcagccggc gcaaggcctt
      121 cggctattcc ttcctgtccg ggctctcaga acccgtgggc gcgctcctgg gatttgttct
      181 gctccggatc tatttcagcg gcgcggtgat gggggcgagt ctcgccgccg tcgccgggat
      241 catggtgttt atctcgctcg acgagctcct gccggccgct cgagagtacg gccagccgca
      301 tctggcgatc tacggcctgg tggcgggcat ggccgtcatg gccatgagcc tgctcatgct
      361 ccactaagcg cgggcgagtc tcatcacccc gtttttcgca atacgatgat cgtcaggtcg
      421 tcattgaggg tgccgcgctc gttcacaaac gaatagaaat cctcgcagag tccggaaagc
      481 gccgcctggg cgcttgtgtc ggacgcgcgc aacaacgacg ccgagatacg ctctcggccg
      541 tagagctccc cctcggcatt gcgcgcctcg gaaaggcagt cggtgtacag cagcaccagc
      601 tcccccgcct ccacgacgaa cgcgaccgca tcgtactcct ggggcgcgct ggatataccc
      661 aggaagaagc cgcccggatg ctcgtccgtt tccggacgga ccggctcgac cccgcgggcg
      721 gtccttctga cgatctcctg gtgggcggcg ttcacatatt ccacgcggtc gccatcgatc
      781 cgcagaagca ccccggtcaa gtagtactgc gactgcccaa tatcgccgaa gagctcatga
      841 ttgaacgcgg tcaggacgga acccagcgga cccgcgagac cgctcacgaa cagacggtag
      901 agcagcgact tggccagcat cgtgatcagc gccgacgata tgccgtggcc ggacacatcg
      961 aacagcgcga ccccctcgag ccgcccgtcc cggacgtaaa aatcgtagaa atcgcccgac
     1021 acgccgctca tgggtctgaa gtaaaaagcg atctcccatc cctcgacgct cggctccgcg
     1081 ccgggaagaa cgctcatctg cacgttcgcg gccagccgca tgtcgtgcgc ggcgatccgt
     1141 tgcgcctggc gaagcgtgtc gttcgtatcc cgcaggtctc ggttcgcctc ctcgagcctg
     1201 aactgcacgt cgaacaacgc gtccttctgg cgctcgagct cgtttttcag cctcacgacc
     1261 tgccgcgtgc gcagataggc gatggtcccg ttgatggacg gaaacagtat catgagcggc
     1321 aagcacgcaa gtgcggtcat aaggctgatc ccggcgtcga cccgaaagcc cgtcaccagt
     1381 ccaccggcaa gcgccgccgc cgcgtacgcc gccaggcacc tggcgagcag ccgggagcct
     1441 cccacggcga tcgcgtccat catgagcatc gagaacatga ccggccccac cagcgggtta
     1501 aagccgatcg cggcgcacca ggcgcccccc agcacgctgt cgatctgcag gtttcgaagc
     1561 tccgcacggt tggagttgtc cgcgcgccgc gcgatccagt acgcgacatg gggccaggca
     1621 aaacaggtca gcgcgagcgc gccccagcac gcggccacgg cgtcatgttg atagagcaat
     1681 gccgcgaggg gaagcgcggc gaggacaaag ccgagatttc gcggcccata aagacgacgg
     1741 gaaagattga atccgctgcg ggatcgggcc tggttcatat gtctatcgtg gtgcgcatga
     1801 tggccttcgg cacgatgcta ccgtcgtccg ggcgcccctg tcaagcagag aatcccgaag
     1861 acgaacgccg caggcgaatc atacaatgcg cttgacataa atataccgat cggtatattt
     1921 tgcccccatg cgccgatccg ctgcctccgg gccgcgcccg gcacatcagc acggggaaag
     1981 agaatgacgg caatcgtcgg aacaaaatcg gaacgcacgc gggcgaggat cgtcgcgcgc
     2041 gtggccccgc tcttcaaccg gaagggctat gcgggcacct cgctgtcgga catgaccgag
     2101 gccgcgggac tcaccaaggg cgccatctac gggaacttcg acggcaagga cgaggtggcg
     2161 ctccacgcgt tccggcacaa catcggcggg atcatcgcgg agctggaggg cgcggtccga
     2221 tcgcgtgacc ggtgcgcgga caagctccgg gcctacaccg agacctatcg gcgcatctat
     2281 cccgcaatcg ccgcgggggg cggctgcccg ctcgcaaata cgctctgcga cgccgacgac
     2341 acgcacccgg gactgcgcgc ggaggcggga cgcatgctcg cgcgctggcg ggacacgatc
     2401 gcccgcatcg tcgcgcgcgg catcgcctcc ggcgagtgcg ccccgtcggc cgacccgcgc
     2461 cgcgcggcca tgaccatcat caccctgatc gagggcgggg cgctgctggc ggcgggcgcg
     2521 cgctccgcgg attacttcat gaacgcgctg gaccgggtcg acgactacat cgacgcgctg
     2581 tgccggcacg acgacggggg cgcgcccccg cagcgctata tcacaaccag gagacgacga
     2641 tgagcgacat ctaccgcgaa ctgcagcaac ggctggacac ctactccgtg ggcttccccg
     2701 cgaccgaatc gggcatcgag atccggattc tgaaaaaact cttcaccgag gaggaggcgc
     2761 ggtttttcct cgcgatgagc cccctgctcg aggcgcccgc gtccgtcgcg gaacggaccg
     2821 gcatgccggt tgacgcggcg gccgcgcggc tcgaagacat ggcctcccgc gggctgctct
     2881 tccgtcagaa aaagggcgat tccgcgcgct atggggcgat cccgttcgtg cacggcctca
     2941 tcgaattcaa ggtgaaggac ctcgatcccg agctcgcggc gctttttttg caatactccg
     3001 acgagggctt cgaccggtca ttcgtgaacg tcaagggcgc ctttttacgc accatccccg
     3061 tgcggcaatc catcaccccc gagcaccatg ttgccgcgct cgacgacgtg gagcagatcc
     3121 tcgcggggat cgacacgatc gtggtcaccg actgcatctg cagaaaagga aaggcgatgg
     3181 tgagcggctc ctgcggcgcg ccggtggagg tctgcttcat gttcggctcg atggcgcgct
     3241 actatctcga caataacatg ggcaggcgag tcaccgccga agaggcgctc gcgctcacgc
     3301 gcacggccca ggaggcggga ctggtcaccc agccgtcaac ctcgcagaac ccgaacggca
     3361 tgtgcaactg ctgcggcgac tgctgcggga ttctctcctc gatcaagaag acgccccgcc
     3421 ccgccgatct ggtgtacacg aatcattttt cggaggtgat cgccgacgaa tgcgcgggat
     3481 gcgaggactg catcgcgtgg tgtcccatgg ccgcgatctc gatggacgcc gacgggacgg
     3541 cccgcatcgc gctcgagcgc tgcatcggct gtggggtgtg cgtgcctaaa tgcccggccg
     3601 acgccatcgt cctgcgcgcg aaacccgagg gcgagcgcag ggcgcttccg aaaaacgcgc
     3661 tcgagcaaat gatgctgatg gcgcaggaac ggacgcgcta gcggctacag cgccagcgcc
     3721 atcagccgcc gcgccccggc gagcccgaac cagagcgcga agacgaccag ggcaacggcg
     3781 cacaccccga tgagcgcacg atatgcgcga tcgccgatga agcgccgtcc ccgggcgatg
     3841 ccgaaggcca cgaaggaata ccaggcgaga tccgcgagga tgtgcccggt aaaaaacgag
     3901 gccacgccca gcgccccttc ccgcatggag tacgtgatat aacccaggcc gatggtggcc
     3961 caccagatgc tccagtacgg attggccacg ctcatgacga tccccgcgag gaccggatgc
     4021 atccccgacg aggtcccgtc cccgctcgag atgcggagtt cgggaagcgc gcgaaacatc
     4081 ccatacgcca tccacagcat caccagcgcc ccggcggcgc cgatgacgcc gaaggccgca
     4141 tccgtcgcga ggagcgcgcc gagccccacg agcaaaagcg ccacgagcgc acactcgagg
     4201 atcgcatgac cggcgatgag gatcggaccg gcccaaaacc cgcgcgctgc ggcctcccga
     4261 acggtgaccg cgaggagcgg acccggcatg agcgcgccgg aaaaggcgac cgcgaacgag
     4321 gcgccgaaaa tccccacgag cgtcagcagc gtatcacccc ccatgcgttt ctcctccctt
     4381 ttctctccgt tccgcgccat ccattgcgcg catcgccgat acggcctgtc aaaaataatt
     4441 ttactgaaaa tcaattgaca aaatgccgcg acggcggggc actaaaagaa aacataccat
     4501 caggtggcgg caatgggact cttcggaccg aatatcgaga aactcatcga caacgacgat
     4561 gttgacggcc tgctcaagct cctgggccac cgcaagccga aaatccgcct gggggcgctc
     4621 ctggcgctct cgcgcaagcg cgacgag
//
LOCUS       DAORHF010000276         4640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19572_length_4640_cov_5.948746, whole genome shotgun sequence.
ACCESSION   DAORHF010000276 DAORHF010000000
VERSION     DAORHF010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19572_length_4640_cov_5.948746"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2223
                     /locus_tag="PKJ16_16560"
     CDS             <1..2223
                     /locus_tag="PKJ16_16560"
                     /inference="COORDINATES: protein motif:HMM:NF019122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48658.1"
                     /translation="LPGVALPPSITATNDIAAAVDDADAIIFGTPSKAIYPIAESMAG
                     RVPPDAILGYLTKGFCKLHGRIYTISEALGIALPAFRERVVAIYGPSHAEEVCVRYHT
                     CLNIAGRHREACEAFRELMACEYLECRVVDDILGVELGGTLKNPAAVAAGMLANLPRC
                     GDNMAGALIAESFKEILRVARACGAKDETMFDISGLGDLITTALSDHSRNRRFGKDIA
                     LQLQKSGTTMRFYDRIIMRFRPDYIFDKMSRRLHYLAEGAYAIEPLLQLAAQKGVSIP
                     VYRSLYEILINNRSPDLLIETVKKPEKFEELYRTSALRVTKRKKGLENTGGTTFRSII
                     ARRVVERCAEDENLRRYILEYRTSFVRRYDRASASRRDILGRLFSVQEVWLMRALTEE
                     SLPRTAETLAKQYLGEFSDRFSLLAAVVFYLVIGLRNIPNRLLRRNYFKKMFRNNIRG
                     SGDIARLRSVSDLANVIYLPVARSYFDFIYVTMILRRHSLPYPRFFVNGAVVTNAFRR
                     MVLRLTGGYIVDFRRFVNPLYRETFRQYFLTLVDHGVPVLFCPELTVSKDGSIRELHE
                     HFIRGIVGFIRKNAEEIALVPVGVSHFAKPAELLTSRSSGSLPLARILGNTIAVHFSG
                     PIMLSEVAARENAVGLIAEMIRSSWARDSFVFAHYVFCRYLRDRGYSVAVRDGEDDLA
                     RYLSAAGERSGIAPRKIFKRGMQFCVRNGIATVSGGTLVVSARDEVDYYATLAPTLTA
                     "
     gene            complement(2213..3169)
                     /locus_tag="PKJ16_16565"
     CDS             complement(2213..3169)
                     /locus_tag="PKJ16_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48659.1"
                     /translation="MDEQLQQLVVSISTLIRKVSEGAISPEDARDEYRSLRVRHDDLR
                     DRAVDAQGGVIKNLCARHNGENFSLFSGLFRLTPEHCEPIDAVGDDRLLGKVIQASAE
                     IRASIRGGSPARIEAGRLPFLAHHLHVARVHSGAGEEILFAAVSSSEFFSRDRFVHAA
                     GFFSDLLAPFLRASRPLMLVQFERSLEAIRDHLDATVNAATSARISLFFLKSVDTIFS
                     HMGMAALVEIPKTIHGILSEVAPEDAAVFSLSMNEYLVISPIRRSDESIPKMRPVELK
                     FRGTIIPHHSIQLRFDDGDSVYKLLDDIYNFEDYMLTGDITR"
     gene            complement(3221..>4640)
                     /locus_tag="PKJ16_16570"
     CDS             complement(3221..>4640)
                     /locus_tag="PKJ16_16570"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HNV48660.1"
                     /translation="VDEDPGAAGRHARRPHGRPRVARVRPAMNLEKRLAGLINNEARA
                     HGFVDYSPDHIARLLELHGNPHRRLAAVHIAGTNGKGSTAHMIARIAQTAGYKTGLYT
                     SPHLLRIHERIRIQGRPIPDSALNRLADMAFNALAAHPDIAVTYFDALTFIAFAYFAE
                     ERVDLAVIETGLGGRLDSTNVVTPRVSVITDIALDHTRVLGSTIPQIAREKAGIIKPG
                     VPVVTTNTAPGTVAVLADACRGQRSALFRYGHDFFARLSGEKGEDRTVIDISAAVGSS
                     PPINLQRLPFDGRAPVQMKNAAAAIATALCLTARGFTITPAHMRAGVSARVPGRLQLL
                     SKSPPVLFDPAHNPAAVRAVADAIRASYPDRRVHAVVSFMRDKDAAGMVRALAPLCAS
                     LHYLNIDDARSRTITPGAAGRAWRNMLVPIDTLDALATRLSRMCAPTDLVVFTGSFRL
                     YRHARDFARLRAKAASAPAPFF"
BASE COUNT          816 a         1433 c         1552 g          839 t
ORIGIN      
        1 ctgccggggg tcgcgctccc gccgtcaatc acggcgacga acgacatcgc cgcggcggtc
       61 gacgatgccg acgcgatcat cttcgggacg ccgtcgaagg ccatctatcc catcgccgag
      121 agcatggccg ggcgcgtgcc ccccgatgcg atcctgggtt atctcaccaa gggattttgc
      181 aaacttcatg gacgcatcta tacgatttcc gaggcgctgg ggatcgccct gccggcgttt
      241 cgcgaacggg tggtcgccat ctatggcccc agccatgcgg aggaggtgtg cgtccgctat
      301 catacctgcc tgaacatcgc cgggcgccac cgtgaagcgt gcgaggcctt ccgggagctg
      361 atggcctgcg aatatctgga gtgccgcgtg gtcgacgaca tcctgggggt cgagctgggc
      421 ggaacgctca agaacccggc ggcggtcgcg gcggggatgc tcgccaacct cccccgatgc
      481 ggggacaaca tggccggcgc gctcatcgcc gaatccttta aggagatcct tcgcgtcgcc
      541 cgggcctgcg gcgcgaagga cgagaccatg ttcgacatct cgggcctggg ggacctcatc
      601 accacggcgc tcagcgacca cagccgcaac cgccgcttcg ggaaggatat cgccctgcag
      661 ttgcagaagt cgggcaccac gatgcgcttc tacgaccgta tcatcatgcg ctttcgaccg
      721 gactatatct tcgataagat gagtcgtcgc ctgcactatc tcgcggaggg ggcgtatgcg
      781 atcgagccgc tgctgcagct tgccgcgcag aagggggtga gcatcccggt ctatcgttcg
      841 ctctatgaga ttctcatcaa caaccgctcg ccggacctgc ttatcgagac ggtgaaaaag
      901 cccgagaaat tcgaggagtt gtaccgcacc tccgcgttgc gcgtcaccaa gcggaagaag
      961 gggttggaga acacgggcgg gaccacgttc cggagcatta tcgcccgccg cgtcgtggag
     1021 cgctgcgcgg aggacgaaaa cctgcgtcgc tacatcctgg agtatcgcac gtcctttgtt
     1081 cgccggtacg atcgggccag cgcgtcgcgc cgggacatcc tcggccggct cttctccgtg
     1141 caggaggtgt ggctcatgcg cgccctcacc gaggagagcc tcccgcggac ggccgagacg
     1201 ctcgccaagc aatacctggg cgaattctcc gaccgcttca gcctgctcgc cgcggtggtg
     1261 ttctatctgg tgattggctt gcgcaatatc ccgaaccggc tgttgcggcg gaattatttc
     1321 aaaaaaatgt ttcgaaacaa tatccgcgga tcgggggaca tcgcccggtt gcgaagcgtc
     1381 tcggacctgg ccaacgtcat ctatctgccc gtcgcccgga gctatttcga tttcatctat
     1441 gtgaccatga ttctgcggcg tcactcgctc ccgtatccgc gctttttcgt gaacggcgcc
     1501 gtggtgacca acgccttccg ccggatggtc cttcgcctca ccggagggta tatcgtcgat
     1561 tttcgccgtt tcgtaaatcc gctgtatcgc gagaccttcc ggcagtattt tctgacgctg
     1621 gtggaccacg gcgtgccggt gctcttctgt ccggagctca ccgtgagcaa ggacggcagc
     1681 atccgggagc tgcacgagca cttcatccgc gggatcgtcg gttttatccg caagaacgcc
     1741 gaggagatcg cgctcgttcc ggtcggggtg tcgcatttcg cgaaaccagc cgagctcctc
     1801 acgagccgct cgtcgggatc gctcccgctc gcgcggatcc tgggcaatac catcgcggtg
     1861 catttctcgg gacccatcat gctctcggag gtggccgcgc gcgaaaacgc ggtcggactc
     1921 atcgcggaga tgatccgctc gagctgggcg cgcgattcgt tcgtgttcgc gcattacgtc
     1981 ttctgccgct atctgcggga ccgggggtat tcggtcgcgg ttcgcgacgg cgaggacgac
     2041 ctggcgcgct acctctcggc cgccggggag cgctccggga tcgcgccgcg gaagatcttc
     2101 aagcggggca tgcagttttg cgtgcgcaac ggaatcgcaa cggtgtccgg cgggacgctc
     2161 gtcgtcagcg cgcgcgacga ggtggactac tacgcaaccc tcgctccgac gctcaccgcg
     2221 tgatatcgcc ggtgagcatg taatcctcga aattgtagat gtcgtccagg agcttgtaga
     2281 cggagtcgcc gtcgtcgaac cgcagctgga tgctgtgatg gggaatgatg gtgccgcgga
     2341 acttcaactc caccggccgc atcttcggga tcgactcatc gctccgccgg atcgggctga
     2401 tgacgaggta ctcgttcatc gaaagcgaaa acaccgcggc gtcctccggg gcgacctcgc
     2461 tcaaaatccc gtgaatcgtc ttcgggatct ccaccagcgc agccatgccc atgtgggaga
     2521 agatcgtatc gacggatttc aggaagaaga ggctgatgcg cgccgaggtc gccgcgttga
     2581 ccgtcgcgtc caggtggtcg cgtatcgcct ccagggagcg ctcgaactgc accagcatga
     2641 ggggacgtga ggcgcgcaga aacggcgcga gcaggtccga gaagaacccc gcggcgtgga
     2701 cgaatcggtc ccgggaaaaa aactcggatg acgatacggc ggcaaaaaga atctcctcgc
     2761 ccgctccgga atggacgcgc gccacgtgca ggtgatgcgc gagaaagggc agccgtccgg
     2821 cctcgatgcg ggcgggcgat cccccgcgta tcgaggcgcg aatctcggcg ctcgcctgta
     2881 tcaccttgcc caggagccgg tcgtcgccga ccgcatcgat gggctcgcag tgttcggggg
     2941 ttaggcggaa gaggccgcta aaaagcgaga agttctctcc gttgtgccgc gcgcagaggt
     3001 ttttgatcac gcctccctgg gcgtcaaccg cgcggtcgcg cagatcgtca tggcgcacgc
     3061 ggagcgaacg atactcgtcc cgtgcgtctt cgggggaaat ggcgccctcg ctgaccttcc
     3121 tgatcagcgt gctgatcgaa acgacgagct gttgcagttg ttcatccatt gacgggccgc
     3181 gttcgccggc tattggcgca ttcatcggcg agttttgcaa tcaaaaaaac ggcgcggggg
     3241 ctgatgcggc cttcgcgcgc aggcgcgcga agtcgcgtgc gtggcgatac agccgaaagg
     3301 acccggtaaa gaccaccagg tccgtgggcg cgcacatgcg cgacaggcgc gttgcgagcg
     3361 catcgagggt gtcgatggga acaagcatgt tccgccacgc ccggcccgcg gcgccgggcg
     3421 taatcgtgcg gctgcgcgca tcatcgatgt tcaggtagtg cagcgatgcg cagagcggcg
     3481 cgagcgcccg caccatgccc gccgcgtcct tgtcccgcat aaacgaaacg accgcgtgaa
     3541 cgcgccggtc cgggtatgac gcgcggatcg catcggcgac ggcgcggact gcggccggat
     3601 tgtgcgccgg atcgaacagg accggcggcg attttgaaag gagctgcaga cggccgggga
     3661 cgcgcgcgga aacgccggcg cgcatatgcg cgggcgtgat cgtaaatccg cgggcggtaa
     3721 ggcagagcgc ggttgcgatc gcggcggctg cgtttttcat ctgcaccggc gcgcggccgt
     3781 cgaagggcag gcgctggaga ttgatcggcg gggaagaacc gaccgccgcg ctgatgtcga
     3841 tcaccgttcg atcctctccc ttttcgccgg ataggcgcgc gaagaaatca tgtccgtatc
     3901 ggaacaatgc tgaacgttgg ccccggcagg cgtcggcaag caccgcgacc gtaccggggg
     3961 ccgtattggt ggttacaacc gggacgcccg gcttgatgat acccgccttc tcgcgcgcaa
     4021 tctgcggtat cgtcgagcca agcacccggg tgtggtccag cgcgatgtcc gtgatcacgg
     4081 aaacacgcgg cgtgacgaca ttcgtagaat cgagccgccc gccgagtccc gtctcgatca
     4141 ccgccaggtc cacccgctcc tcggcaaaat acgcgaaggc gatgaaggtg agcgcgtcga
     4201 agtaggtgac ggcgatgtcc gggtgtgcgg cgagcgcatt gaacgccatg tccgcgagac
     4261 ggttgagcgc ggaatccgga atcggccgtc cctgtattcg aatgcgttcg tggatgcgaa
     4321 gcaggtgggg cgaggtgtac aggccggttt tatatccggc ggtctgcgcg atacgggcga
     4381 tcatgtgcgc cgtagagccc tttccgttgg tgccggcgat gtgaaccgcg gcgagccgac
     4441 gatgcgggtt cccgtggagc tcgagcaggc gcgctatgtg gtcgggggaa tagtcgacga
     4501 agccgtgcgc gcgcgcctcg ttgttgatga ggccggcgag ccgtttctcg agattcatcg
     4561 cggggcggac gcgcgcgact cgagggcgcc catgagggcg tcgcgcatga cgtccagcgg
     4621 cgccggggtc ctcgtccaca
//
LOCUS       DAORHF010000277         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19653_length_4628_cov_5.384649, whole genome shotgun sequence.
ACCESSION   DAORHF010000277 DAORHF010000000
VERSION     DAORHF010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19653_length_4628_cov_5.384649"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PKJ16_16575"
     CDS             <1..252
                     /locus_tag="PKJ16_16575"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48661.1"
                     /translation="TFTGQWVDGKIHGRGAMVWENGARYSGQWRNGKRDGRGSFTWPK
                     GASYVGEWRDNNRHGRGTHTDEDGTVRAGRWKEDAYLGK"
     gene            233..751
                     /locus_tag="PKJ16_16580"
     CDS             233..751
                     /locus_tag="PKJ16_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48662.1"
                     /translation="MRILENDTRARARGERSRLALVALAAAAFMWSACSTPGANESTP
                     AGDIREGWVDDEAYRFVHTGRSKVGTDNAPESDRSRTACDAALMMAQARAIEVLAEAG
                     LKNVSGTVTVTEYRDRIVKEIQGFVRGGRVVSQAFSPVDNTCTIVYEIREKDLKKRVL
                     MAAEKNKPPVSY"
     gene            916..1407
                     /locus_tag="PKJ16_16585"
     CDS             916..1407
                     /locus_tag="PKJ16_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48663.1"
                     /translation="MKIRIHAIVVAAIAVAIIGCATGAPVTPDMEEELKKKVGYWVDN
                     HTFVVIANGQPTRELVEKKDRRHTSRRDAILFTQKWVLDRFYRMRISAITEKPIDYRI
                     VEISLMNELARMGIVVGTKDDSVRLPRTWTVLRELYDRDDACEMIVQIRYKSLKDIIY
                     RTK"
     gene            1411..3537
                     /gene="waaF"
                     /locus_tag="PKJ16_16590"
     CDS             1411..3537
                     /gene="waaF"
                     /locus_tag="PKJ16_16590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HNV48664.1"
                     /translation="MPGLRRSLNILHVGTALTWRGGEAQILYLARGLAGMGHSVTVAT
                     PRKSVLYDKSVEAGIGVYAFAPRSELDIVAALRLARYIRKNNIHVLHMHDAHAHTIGV
                     MAMKFCRPLITVLARRVDFRVAGNFLSRVKYRAPIDRIIAISEGVRSVLVDCGIPREK
                     IELVYSGIQFPKEKYSPEVRREFGIAPSEKIVGIVAALAPHKDHENFLAAAAVVRRAH
                     PAVRFMVVGDGELEEPLRARARELGIDDCVIFTGFRTDVHRIMAAFDVFVLSSYLEGL
                     CTSILDAMALALPIVATDAGGIPELVINEKTGLLVPPRDSNALAGAIAALLANKTRAR
                     ALAKAAYAKVQHFSNVAMYTGTEKVYQRTLDEKFSRIVVRTPNWIGDAIMSTPFFAAL
                     REAWPAARITALAKPKLAEVYWNNPDVDDIITFHEAADAVASIKPRRFDLGIIMPDSF
                     SSASLFWRARLPYRFGYRGDGRSFLLTNPQRKSMDDVTESYVGLANALGATRAAGPWR
                     LFVSADEDHAIIKKMAGFGLSTRDTLVGVNPATAFGPSRIWPLERYAELARRMIAAHR
                     AKVIVFTGPGEDSFADYFRNSCADCGKNLVVLGGEDRLSLREYMAVMSHLRAFVTNDT
                     GPMHIALARGVPTVALFGSSDPEKEVFREHRAGNVLIHHTLPCSYCYDRTCESGRDYE
                     CLTSVSVDEVAAAVDGLLRAKTKRRR"
     gene            complement(3548..3802)
                     /locus_tag="PKJ16_16595"
     CDS             complement(3548..3802)
                     /locus_tag="PKJ16_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HNV48665.1"
                     /translation="MDFITLLGLLAGVLTTTAFVPQVVKTWRTKSAEDISLVMFIMLV
                     TGIVLWLAYGLLIRSLPLILSNGIAVFLTGSILYFKLRYE"
     gene            3939..4319
                     /locus_tag="PKJ16_16600"
     CDS             3939..4319
                     /locus_tag="PKJ16_16600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48666.1"
                     /translation="MDEKERIELIRKGNELFNKGDIAAATEIFTKTKYRDGLTRVGDY
                     YFYQQRRPLVAFTYYKMVNKTEKINEIFERMMFALGKLIRGDAAPDEETAKVSLPPLK
                     VSPKLKILAEEILRDQEQCDGPKR"
     gene            4357..>4628
                     /locus_tag="PKJ16_16605"
     CDS             4357..>4628
                     /locus_tag="PKJ16_16605"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HNV48667.1"
                     /translation="MNTLKKTIVGVLTLLAAARADAAIRDAGAYLRAQVVQRTLSNGI
                     EVLMLDRGAAPTLALVISFRVGSADESYDTIGMAHMLEHMLFKGTVT"
BASE COUNT          882 a         1470 c         1505 g          771 t
ORIGIN      
        1 accttcaccg gacaatgggt cgacggcaag attcacggcc gtggcgccat ggtctgggaa
       61 aacggcgcgc gctacagcgg ccagtggcga aacggcaagc gcgacggacg agggagtttc
      121 acctggccca agggggcgtc ctatgtcggg gaatggagag ataataatcg ccatggccgg
      181 gggacccata cagacgaaga cgggaccgtc agggcgggga ggtggaagga agatgcgtat
      241 cttggaaaat gacacacggg ctcgcgcgcg gggcgaacgt tcccgcctgg cgctggtggc
      301 gcttgccgcc gcggcgttta tgtggagcgc gtgttctacg ccgggcgcga acgagtcgac
      361 gccggccggg gatattcgcg aggggtgggt ggacgacgag gcctatcgct tcgttcacac
      421 cggccgcagc aaggtcggga ccgacaacgc gccggagtcc gaccgcagtc gcaccgcctg
      481 cgatgcggcg ctcatgatgg cccaggccag ggcgattgag gtgctcgccg aggccggtct
      541 caagaatgtg tcgggtacgg tcacggtcac ggagtaccgg gaccgcatcg tcaaggagat
      601 ccaggggttc gtgcggggcg ggcgcgttgt gtcgcaggcc ttcagcccgg tggacaatac
      661 ctgcaccatc gtatatgaaa tcagggaaaa agaccttaaa aagcgcgtgc tgatggccgc
      721 cgaaaagaac aaaccgccgg tttcgtactg agcgacacgg tcggcgtctc gacgcgggaa
      781 agtgcttgtc ggcgggtccg cggatctgta caatagcccg atgcgcgtca atggcgcatc
      841 ccgagccgcc acgatttcga ttcgtgcggt cgagggccga accgcctata ccatgcctgt
      901 gccagggagg agagcatgaa gattcgtata cacgccatcg tggtcgcggc gattgccgtc
      961 gctatcatcg gctgcgcgac gggagcgccg gtcacgccgg acatggagga agagctcaag
     1021 aagaaggtcg gctactgggt cgacaatcat acctttgtcg tgatcgcaaa cggccaaccc
     1081 acgcgggagc tcgtcgagaa gaaggaccgg cggcacacgt cgcgccggga cgcgatcctg
     1141 ttcacccaga agtgggtcct cgaccggttc taccgcatgc ggatcagcgc catcaccgaa
     1201 aagcccatcg actaccgcat cgttgagatc tcgctcatga acgagctggc gcgcatgggg
     1261 atcgtcgtgg gaaccaagga cgattcggtg cgcctgccgc gcacgtggac ggtgctccgc
     1321 gagctctacg accgggacga cgcctgcgag atgatcgtcc agatccggta caagagcctc
     1381 aaggacatca tctaccggac caagtgaccg atgccgggac tgcgacgctc gctcaatatc
     1441 ctgcacgtgg gtacggcgct cacgtggcgc ggcggtgagg cgcaaatcct ctacctcgca
     1501 cgggggctcg ccggaatggg ccactcggtg accgtcgcca cgccgcgaaa gagcgtgctc
     1561 tacgacaagt ccgtcgaggc ggggatcggc gtgtacgcct tcgcgccgcg atccgagctc
     1621 gacatcgtcg cagccctgcg cctcgcgcgg tatatacgaa agaacaacat ccacgtcctg
     1681 cacatgcacg acgcgcacgc gcacaccatc ggcgtgatgg ccatgaagtt ctgccgtccg
     1741 ctcatcaccg tcctcgcgcg ccgggtcgat ttccgtgtcg cgggaaactt cctgagccgc
     1801 gtaaaatacc gcgcccccat cgaccggatc atcgccatct ccgagggggt ccgttccgtg
     1861 ctggtcgact gcgggatacc gcgcgaaaaa atcgagctgg tctacagcgg catccagttc
     1921 ccgaaggaga aatattcccc ggaggtgcgc cgcgagttcg gcatcgcccc gtcggagaag
     1981 atcgtgggaa tcgtggccgc gctggcgccg cacaaggatc acgagaactt cctcgccgcg
     2041 gccgccgtcg tccggcgcgc ccaccccgcg gtgcgtttca tggtcgtggg cgacggcgag
     2101 ctcgaggagc cgctccgtgc gcgcgcgcgg gagctcggga tcgacgattg cgtgatcttc
     2161 accggtttcc gcaccgacgt gcaccgcatc atggcggcat tcgacgtgtt cgtcctctcg
     2221 tcgtacctcg agggcctgtg cacctcgatc ctggacgcga tggcgctggc gctgcccatc
     2281 gtggccaccg acgccggcgg cattcccgag ctggtcatca acgagaaaac gggcctcctg
     2341 gtgccgccgc gcgattcgaa tgcgctcgcc ggcgcgatcg ccgccctgct cgcgaataaa
     2401 acgcgcgcgc gtgcgctggc gaaggccgcc tacgccaagg tccagcactt cagcaatgtg
     2461 gccatgtaca cgggcacgga gaaggtgtac cagcggacgc tcgacgaaaa gttttcaagg
     2521 attgtcgtcc gcacgcccaa ctggatcggc gacgccatca tgagcacgcc gttttttgcc
     2581 gccctgcgcg aggcctggcc ggcggcgcgc ataacggcgc tcgcgaagcc caagctcgcg
     2641 gaggtgtact ggaacaaccc cgacgtcgac gacatcatca cctttcatga ggcggcggac
     2701 gcggtggcct cgatcaagcc gcgccgcttc gatctcggga tcatcatgcc cgattccttt
     2761 tcgtccgcat cgctcttctg gcgcgcccgg ctcccgtatc ggttcggcta ccggggcgac
     2821 ggacgcagtt ttctgctcac gaacccgcag cgaaaatcca tggacgacgt gaccgaatcg
     2881 tacgtggggc ttgcaaacgc cctgggggcg acgcgcgcgg ccggtccgtg gcgcctgttc
     2941 gtgagcgccg acgaggacca cgccatcata aagaagatgg cgggcttcgg cctctcgacg
     3001 cgggacacgc tcgtcggcgt caatccggcc acggcgttcg gcccgtcgcg catctggccg
     3061 ctcgagcggt acgcggagct cgcgcgccgc atgatcgcgg cccaccgcgc gaaggtgatc
     3121 gtgttcaccg ggccgggcga ggattcgttc gccgactatt tccggaactc gtgcgccgat
     3181 tgcgggaaga acctggtggt gctgggcggg gaggaccggc tctcgctgcg cgagtacatg
     3241 gccgtcatgt cgcacctgcg cgccttcgtg acaaacgaca ccggccccat gcacatcgcc
     3301 cttgcgcgcg gcgtccccac ggtggcgctt ttcggctcca gcgatccgga gaaggaggtc
     3361 tttcgcgaac atcgcgccgg gaacgtgctc atccatcaca cgctgccgtg cagctactgc
     3421 tacgaccgca cctgcgaaag cgggcgtgac tacgaatgcc tcaccagcgt atcggtcgat
     3481 gaggtggcgg cggccgtcga tgggctgttg cgcgcgaaga cgaagcgccg gcgctaggac
     3541 cggcggccta ctcgtagcgc agcttgaagt agaggatcga ccccgtgagg aaaaccgcga
     3601 tgccgttgga caggataagc ggcagcgaac ggatcagcag tccgtaggcc agccacagca
     3661 ctatccccgt caccagcatg atgaacatca ccagcgagat gtcctcggcc gatttcgtcc
     3721 gccaggtttt tacgacctgg ggcacgaagg cggtcgtcgt gagcacaccc gccagaagcc
     3781 ccagcagcgt gatgaaatcc atggcctttt tcctcgatgg cgtatcgccg acgtgataag
     3841 ctcccttggc tgagccgcac cctgtcaaat gaattttcgc ggcgcacatc gggtttttgt
     3901 ttgacaatcg gccgccgccg gattatgtga tattaccgat ggacgagaaa gagcgcatcg
     3961 agctgatccg caagggcaac gagctcttca acaagggcga tatcgcggcc gcgacggaga
     4021 tcttcaccaa aacgaaatac cgtgacgggc tcacgcgggt gggggactat tacttttacc
     4081 agcagcgccg gccgctggtc gccttcacct actacaagat ggtcaataaa accgagaaga
     4141 tcaacgagat cttcgagcgc atgatgttcg cgctgggcaa gctgatccgc ggcgacgcgg
     4201 cccccgatga ggagaccgcg aaggtttcgc tcccgcccct caaggtttcc cccaagttga
     4261 agatcctcgc cgaggagatc ctgcgcgatc aggaacagtg cgacggcccc aaacggtgat
     4321 cccgccgcgc ggcccatcat acggaggctc ataatcatga acacgctgaa aaaaaccatc
     4381 gtgggcgtcc tgacgctcct cgcggcggcg cgggcggacg cggccattcg ggatgccggg
     4441 gcctatctgc gggcgcaggt cgtccagcgg acgctctcca acggaatcga ggtactcatg
     4501 ctcgaccggg gcgccgcgcc gaccctcgcc ctggtgatct ccttccgcgt gggctctgcg
     4561 gacgagagct acgataccat cggcatggcc cacatgctgg agcacatgct cttcaagggg
     4621 accgtgac
//
LOCUS       DAORHF010000278         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19654_length_4628_cov_4.529193, whole genome shotgun sequence.
ACCESSION   DAORHF010000278 DAORHF010000000
VERSION     DAORHF010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19654_length_4628_cov_4.529193"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2102)
                     /locus_tag="PKJ16_16610"
     CDS             complement(<1..2102)
                     /locus_tag="PKJ16_16610"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV48668.1"
                     /translation="MERIGDYHIVEKVAETHHSIVYRARRDGSHDTVIIKTLKAVYSS
                     PAEIARFRQEYELISRIRLAGVVEYRGLFAYQNAFALVLEDFDGISLAQARESMTFDT
                     ASFLSIAISLSNSLGELHKRNIVHRDINPRNILINPETGVVKITDFGIAAEFARKDEI
                     VSDRTLVEGTLIYMSPEQTGRMNRPVDYRTDIYSLGITLYELLTGQVPARSSDLLEII
                     YSHIARQPAPPHEVAPSIPVMVSRIVMRMIGKMAEDRYQNAFGVAGDLEECRRRITAT
                     GEIAEFEMGSRDIPLRFTIPQILVGRENEIGRLMASFDEAGEGRKEMLLVSGDPGIGK
                     SSVIFEIHKAIVAKRGYFASGKFDQYRRDVPYSAIIQSFAGLVRQLLAESEKRVNDWR
                     EALLSALGPNGRIITDVIPDVELIIGPQPEVPGLSPDESQNRFGFVFRNFVRVFAQAE
                     HPLALFLDDLQWADSASLRLIKTIVADPDIAHLLVIGTYRSNEVGDMHPLVGAIEEVR
                     AAGVPVREIPLRPLTTGHVNTLIAQSLRASEGVSMPLADIVHRKTAGNPFFVHQFLRM
                     IHDRGLLELDPARGWSWDIDAVNRLQVTDNVVDLMTAMIDMLDPAVRADLTAGACIGN
                     EFDVEVVARFANAPIETVVSHMTVALDAGLLNRSGSTYRFQHDRVQEAVYSLVPDAAK
                     ADLHYRIASFFLENTSVE"
     gene            2632..4536
                     /locus_tag="PKJ16_16615"
     CDS             2632..4536
                     /locus_tag="PKJ16_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HNV48669.1"
                     /translation="MSGAYQKAYDLAAKDPAAFWGEAANDIHFYKKYEKVLDDSNKPF
                     YRWFTGGEFNTCYNAVDIHVKNGRGAQTAIIYDSPVTDVKSKFTYQELLDQVSKFAGV
                     LAAQGVSKGDRVIIYMPMIPEAAFAMLACARIGAIHSVVFGGFAPNELAIRIDDAKPK
                     AMVSASCGIEGKKVIEYKPLLDKAIELAKHKPEKCVIYQRPQATSPLISGRDIDWAEA
                     MKSAKPVDCVPVKTTDPLYILYTSGTTGIPKGVVRDNAGHAVALRWSMKNIYGVEPGD
                     VYWAASDVGWVVGHSYIVYAPLLTGCTTIMYEGKPVGTPDPGAFWRIISEYKVKVLFT
                     APTAFRAIKKEDPNGDHFKKYDLSNFKYLFLAGERLDPDTYHWASDLLKKPVIDHWWQ
                     TETGWSIAANCMGLEPFPIKAGSPTKPVPGYQVEILDAEGKKLPNGTEGMVAIKLPLP
                     PATLPTLWQNDDKYKESYLDMYPGYYFTGDGGYYDTDGYIYIMGRVDDVINVAGHRLS
                     TGAMEEIIATHPDVAECAVIGAADTFKGQLPLGFVVLKAGVTKDEKAILDELVKMIRD
                     KIGAVACYKQSLIVKRLPKTRSGKILRGTMRKIADGETYTVPSTIDDPEILTEIAGYV
                     KGVGYGVKQG"
BASE COUNT          950 a         1475 c         1370 g          833 t
ORIGIN      
        1 tcctccacgg aggtgttctc cagaaaaaat gaggcgatgc ggtagtgcag atccgccttc
       61 gccgcatcgg ggacgagcga atagaccgcc tcctggacgc ggtcgtgctg aaaccgatag
      121 gtggaccccg accggttgag cagccccgca tcgagggcga ccgtcatgtg cgacacgacc
      181 gtttcgatgg gcgcgttcgc gaaccgggcc accacctcca catcgaactc attgcctatg
      241 caggcccccg ccgtaaggtc cgcacgaacg gcgggatcga gcatgtcgat catcgcggtc
      301 atcaggtcaa cgacgttgtc ggtcacctgc aggcggttga ccgcgtcgat gtcccacgac
      361 cagcccctgg ccggatcgag ttcgagcagg ccccggtcgt ggatcatccg gagaaactgg
      421 tgcacgaaga acggattgcc cgcggtcttt cggtgcacga tgtccgccag gggcatcgat
      481 acgccctcgg aagcgcgcag gctttgcgcg atcagcgtgt tgacgtggcc ggtggtgagc
      541 gggcgcaacg ggatctccct gacgggcacc cccgccgcgc gcacctcctc gatcgctccc
      601 accagcggat gcatgtcgcc gacctcgttg ctccggtatg tcccgatcac caggaggtgc
      661 gcgatatcgg gatcggcgac gatcgtcttg atgagcctga ggctcgcgga atccgcccac
      721 tgcaaatcgt cgagaaacaa ggccaacgga tgctccgcct gcgcgaacac ccgcacaaaa
      781 ttccggaaca cgaacccaaa ccggttttgc gattcgtccg gggaaagacc gggcacctcc
      841 ggctgcggcc cgatgatgag ctcgacgtcc gggatcacgt cggtaatgat tctcccgttg
      901 ggacccagcg ccgaaagaag ggcctcccgc cagtcgttga cccgcttctc gctctcggcc
      961 agcagctgcc tgaccagccc cgcgaacgac tggataatcg cgctgtacgg cacgtcgcgc
     1021 cggtattggt cgaacttacc ggaggcgaaa tatccccgct tcgcgacaat ggctttgtgt
     1081 atctcgaaaa tcaccgacga tttcccgatc ccgggatcgc ctgaaaccag cagcatctcc
     1141 ttccgcccct cgccggcctc atcgaaggac gccataagcc gcccgatctc attctcgcgt
     1201 cccacgagga tttgcgggat ggtgaatctc agcggaatgt cccgcgaccc catctcgaat
     1261 tcggcgatct ccccggtcgc cgttatgcga cgccggcatt cttcgagatc gccggcgacg
     1321 ccgaacgcgt tctggtagcg gtcctccgcc atcttcccga tcattcgcat gacgatgcgg
     1381 gagaccatca cgggaattga cggcgccact tcgtgcggcg gcgccggctg tcgcgcgata
     1441 tgcgaataga tgatctccag caggtcggac gaccgcgcgg ggacctgccc ggtcagcagc
     1501 tcgtacaggg tgatgcccag cgaatagatg tccgtgcggt aatcgacggg gcggttcatg
     1561 cggcccgtct gctcggggga catatagatc agcgttccct cgacaagcgt ccgatcggag
     1621 acgatctcgt ccttgcgcgc aaattccgcc gcgatgccga agtcggttat tttcacgacg
     1681 cccgtttcgg ggttgatgag gatattccgg gggttgatat cgcgatgtac gatattccgc
     1741 ttatgaagct cccccaggct gtttgaaagg gaaatcgcga tcgaaagaaa tgacgccgtg
     1801 tcgaatgtca tgctctcccg cgcctgcgcg agggagatgc cgtcgaaatc ctcgaggacg
     1861 agagcgaagg cgttctgata tgcgaacagc ccgcggtact cgacaacgcc cgcgagccgg
     1921 atcctgctga tgagctcgta ttcctgtcta aagcgcgcga tctccgccgg cgacgaatag
     1981 accgccttca gcgttttgat gatgacggta tcgtgcgacc cgtccctgcg ggcgcgatag
     2041 acaatcgagt ggtgtgtttc cgcaaccttt tcgactatgt ggtaatcgcc gattcgctcc
     2101 atccaaacct ttcgcgcagt tgaattccgc gcggacagta tccgcacagc cgctcattcg
     2161 catcctgatg catatatcga cgtcgaacat gcgcttgaac ccatgcccgg ttcattgaga
     2221 ggcgacagaa ccgaacgaca ttcacattca taaacacggt ttcagcgaaa gcgtgctttc
     2281 taattatgta tgatctgcgc gcatgcgcac agcacgaatg agaaccgtcc gccgcccggt
     2341 atgtatcccg ctaccaacgt gcatacacaa cggcgacttc aatgtcaaac gaaatagtcc
     2401 atcggcgccc aatcggcgcg acggccgcat cgtccggcgg cgaaccgagc gagcgttcgt
     2461 acggtttttt tattgacacc gatggcgcgc ctcataggct tgttgcggat tggccgtgag
     2521 tgcgtgcatc agcaccatat tccccgaaac atacaggcgt gcacatccgg gggacatcaa
     2581 cacacgcgcc cgctgggcgc gcatcatact cttacctgca gggaggtacc tatgtcagga
     2641 gcataccaaa aggcctatga tttggccgcg aaggacccgg ccgcgttctg gggcgaggcc
     2701 gcaaacgaca ttcatttcta caagaaatac gagaaggtgc tggatgactc caacaagccc
     2761 ttctaccgct ggttcacggg cggggagttc aacacctgct acaatgcggt ggacatccac
     2821 gtaaagaacg gccgcggcgc acagaccgcg atcatctacg acagcccggt caccgacgta
     2881 aaatcgaagt tcacctatca ggagttgctg gaccaggtat cgaagttcgc cggcgtgctc
     2941 gcggcgcagg gcgtctcgaa gggcgaccgc gtcatcatct acatgcccat gattcccgag
     3001 gcggccttcg caatgctggc ctgcgcgcgc atcggcgcga tccattcggt ggtgttcggc
     3061 ggcttcgcgc ccaacgagct cgccatccgc atcgacgacg ccaagcccaa ggccatggtg
     3121 tcggcctcgt gcggcatcga ggggaagaag gtcatcgagt acaaaccgct cctcgacaag
     3181 gcgatcgagc tggcgaagca caagcccgag aagtgcgtga tctaccagcg cccgcaggcg
     3241 acctcgccgc tcatttccgg acgcgacatc gattgggccg aggccatgaa gagcgccaag
     3301 cccgtggact gcgtgccggt gaagaccacc gacccgctct acatcctcta tacctcaggc
     3361 accacgggga tccccaaggg cgttgtgcgc gataacgccg gccatgccgt cgcgctccgc
     3421 tggagcatga aaaacatcta cggcgtggag ccgggcgacg tgtactgggc cgcctcggac
     3481 gtcggctggg tggtgggcca ctcctacatc gtctatgcgc cgctcctcac cggctgcacc
     3541 accatcatgt acgagggcaa accggtcggc acccccgacc ccggcgcctt ctggcgcatc
     3601 atcagcgagt acaaggtcaa ggtgctcttt acggccccga ccgcgttccg cgcgatcaag
     3661 aaggaagatc ccaacgggga ccactttaag aaatacgacc tttcgaattt caagtacctg
     3721 ttcctggccg gtgagcggct cgatcccgac acctatcact gggcgagcga cctgctcaag
     3781 aagccggtga tcgaccactg gtggcagacc gagaccggct ggtccatcgc ggccaactgc
     3841 atgggcctcg agcccttccc gatcaaggcc ggctctccca ccaaacccgt gcccggctac
     3901 caggtggaga tcctggacgc cgaagggaaa aaattgccga acggcaccga ggggatggtg
     3961 gccatcaagc tgccgctccc gccggcgacc ctgcccacgc tgtggcagaa cgacgacaag
     4021 tacaaggagt cgtacctgga catgtatccc ggctactact tcaccgggga cggcgggtat
     4081 tacgacacgg acggctacat ctacatcatg ggacgcgtgg acgacgtcat caacgtcgcg
     4141 ggacaccggc tctccaccgg cgcgatggag gagatcatcg cgacgcaccc cgatgtggcc
     4201 gagtgcgcgg tcatcggcgc ggccgacacc ttcaagggac agctcccgct cgggttcgtg
     4261 gtcctcaagg cgggggtcac gaaagacgag aaggcgattt tagatgagct ggtcaagatg
     4321 atccgcgaca agatcggcgc ggtcgcctgc tacaagcagt cgctcatcgt gaagcgcctc
     4381 cccaagacgc gctccggcaa gatcctgcgc ggcaccatgc gcaagatcgc cgacggcgag
     4441 acctacaccg tcccgtccac catcgacgat ccggagatcc tgaccgaaat cgcggggtac
     4501 gtgaagggcg tgggctatgg cgtgaagcaa gggtaacatg taaccataga tcgagaacga
     4561 cgcacaggcg ggaccgggct gcaatccggt cccgcctttt tattgccttt tctccgtcag
     4621 acgctcat
//
LOCUS       DAORHF010000279         4581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_19982_length_4581_cov_3.813080, whole genome shotgun sequence.
ACCESSION   DAORHF010000279 DAORHF010000000
VERSION     DAORHF010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4581
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19982_length_4581_cov_3.813080"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1344
                     /gene="flhB"
                     /locus_tag="PKJ16_16620"
     CDS             181..1344
                     /gene="flhB"
                     /locus_tag="PKJ16_16620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HNV48670.1"
                     /translation="MAAFADDIIRLRPFDDEPKAFVFDLQLFAAEDEGRTEEPTEKKL
                     REAREKGQVAKTTELPQAVVVIFGVMVVFFLGAWIYDAMARLMTYYLTGFTRFSLTER
                     SVLIEFFRMTEVSGKVLLPIFAAVMVAAVLGNIVQVGFQISTHPLKFDWKKIKFDPAT
                     IIKKMFFSKQVGMNLFKSIFKVAVIGVIAYAIIVNEYELIMKTPDIGIALALKSCGIV
                     ALKIIIWTSVILLVLSIPDYIFQRHEFMESLKMTKEEMKEEIKETIGDPHMRARLRQM
                     QMDLVMKNMIREVPKADVVVTNPTHFAVALRYDRFTMEAPMLVAKGVDSMALRIREIA
                     KQNDVFMIENRPLAQDLYKRIDVGDMIPPDLFYAVSLIYAELYKVKGYRQAAI"
     gene            1378..3480
                     /gene="flhA"
                     /locus_tag="PKJ16_16625"
     CDS             1378..3480
                     /gene="flhA"
                     /locus_tag="PKJ16_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013313718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HNV48671.1"
                     /translation="MAEARRLSFLTDNLQWMQKTDILVGIGVISVVSMLVIPLPTVIL
                     DFLLAVSIMSGLLILLIVMFVPRSYDFSIFPSLLLVTTVFRLALNVSSTRLILLQGTA
                     FDGKIIRTFGDFVVGGNYVIGLIIFIILIAVQFIVITKGATRTAEVAARFTLDAMPGK
                     QMSIDSDLSNGIITEEVAIRRRAEIRKEADFYGAMDGASKFVQGDVKVGIVITLINII
                     GGFIIGMVIRGESFDVALKTYTLLSIGDGLVAQIPSLLVTTATGIIVTRAVSDDNFGT
                     DLGNQIAAQPRALLITAGALAASAIIPGFPKISILMLAAGLGALGYILQQTQEETQKK
                     EEIDEKEAQLKEHKPESVMPLMQVDPLEVEIGYSLIPLVDPEQGGTLLDRITNIRRRC
                     ALDMGLVVPPIRIRDNMELEPDSYAVLIKGVEIGRGNLQVGRLMAMDPGGVKERIEGD
                     EFVEPVFGLTAIWIEPDLRNTAESGGYTVVDCPTIIATHLTEIIKRHADEILGRQEVK
                     QIVDNIKSDYPAVVDELLNEKKMSLGEIQRILQNLLRERVSIRNMVTILETLASYVDY
                     TKDAGMLTEYVRNALSRQICLEHVDREGMLSVITVSPEIEGIIRSSIHDDPIEGRIVG
                     LEPDVHGRIVAAFIEAYKKSKDAGFAPVYLVSPQIRSVTFTLLEREIADPFVLGYNEV
                     RPEIKVRVISTALMSTAA"
     gene            3513..>4581
                     /gene="flhF"
                     /locus_tag="PKJ16_16630"
     CDS             3513..>4581
                     /gene="flhF"
                     /locus_tag="PKJ16_16630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HNV48672.1"
                     /translation="MRYVKIKAKNYNEAMMKLKMEYGDEAIPISHKYVKEGGLFNTAL
                     MAKDLVELTAAIQESKPARGLGVKPEKKSHIDFTVGDTTETKPLSSVAPPKAAVDNGP
                     LMDALNGLYDRGMAKTETKSASVGALQDTIRKAAAVAEPRVASAGSDSIAKLEKEFQD
                     LKNMLNRVIETKGAAAEKSAADTAEDDRTMRPVLEILRNNDFDHDECTLMVEEIKNNV
                     SREDLKDRYKIEKTLRELLKNRIVTSGPITTGNRKKVIMFFGPTGVGKTTTMAKLGAI
                     HALREGHRVTFITIDTYRIAATEQLKKYAEIMKIPLRVVSDQSGLKEVLDKEKAEIIM
                     IDTSGRSHKNLMKISEIKSFAD"
BASE COUNT          955 a         1380 c         1443 g          803 t
ORIGIN      
        1 tggccttcgg gatcctcgtg ctcacgacgc cgctcatcgt gaggatcatg caggtctccc
       61 tcgagcggac cttcgggttc gtttcgaaag tgattctata ctggccgcaa taggcggccc
      121 cgcccgccgg gcggagaacg gggacgggga ttcgatcccg ccccggtttc gtggagtgat
      181 atggcagcgt tcgccgacga catcatccgg ctgcgcccct tcgatgacga accgaaggcg
      241 ttcgtgttcg acctgcagct cttcgccgcg gaggacgagg ggcgtaccga ggagccaacc
      301 gagaagaagc tccgcgaggc gcgcgagaag ggccaggtcg ccaagaccac cgagctcccg
      361 caggcggtcg tcgtcatttt cggcgtgatg gtggtctttt tcctgggcgc gtggatctac
      421 gatgccatgg cgcgcctgat gacgtactac ctgaccgggt ttacgcgctt ttcactcacc
      481 gagcgttcgg tgctcatcga gtttttccgg atgacggagg tctcggggaa ggtgcttctg
      541 ccgatcttcg ccgccgtgat ggtggcggcg gttttgggca acatcgtgca ggtcgggttc
      601 cagatctcca cgcacccgct gaaattcgac tggaaaaaga tcaagttcga cccggccacc
      661 atcatcaaga agatgttctt ctccaagcag gtggggatga acctgttcaa gtcgatattc
      721 aaggtggcgg tcatcggggt gatcgcgtac gccatcatcg tgaacgaata cgagctgatc
      781 atgaaaacgc cggacatcgg catcgcgctg gcgctcaagt cctgcgggat cgtcgcgctc
      841 aagatcatca tctggacctc ggtgattttg ctggtgctgt cgatccccga ctacatcttc
      901 cagcgccacg agttcatgga gtccttgaag atgaccaagg aggaaatgaa ggaggagatc
      961 aaggagacga tcggggaccc gcatatgcgg gcgcgcctcc gccagatgca gatggacctg
     1021 gtgatgaaga acatgatccg cgaggtgccc aaggcggacg tggtggtgac caaccccacg
     1081 cacttcgccg tggccctgcg ctacgaccga tttacgatgg aggcgcccat gctcgtcgcc
     1141 aagggcgtcg acagcatggc gctgcgcatc cgggagatcg cgaaacagaa cgacgtgttc
     1201 atgatcgaga accgcccgct ggcgcaggac ctctacaagc ggatcgacgt gggcgatatg
     1261 attccgccgg acctgtttta cgcggtttcg ttgatttacg ccgagctgta caaggtgaag
     1321 ggctaccgtc aggcggcgat ataatgccgc acgggatgaa cgggaattga ggggacaatg
     1381 gctgaagcga gacgattatc gttcctgacc gacaacctgc aatggatgca gaagaccgac
     1441 atcctcgtgg gtatcggcgt gatctccgtg gtctcgatgc tcgtgatacc gctgcccacg
     1501 gtgatcctcg acttcctgct ggccgtgagc atcatgtcgg ggctcctcat cctgctcatc
     1561 gtgatgttcg tgccgcgctc gtacgatttc tcgatattcc cgtcgctcct gctggtcacg
     1621 acggtgtttc gcctcgcgct caacgtgtcc tcgacccgcc tcatcctcct tcaggggacg
     1681 gcgttcgacg gaaagatcat ccggaccttc ggcgacttcg tggtcggggg gaattacgtc
     1741 atcgggctca tcatcttcat catcctcatc gcggtgcagt tcatcgtgat caccaagggc
     1801 gccacgcgga cggccgaggt cgccgcgcgg ttcacgttgg acgcgatgcc cggcaagcag
     1861 atgagcatcg actccgacct ttccaacggc atcatcaccg aggaggtggc catccggcga
     1921 cgcgccgaga tcaggaaaga ggcggatttc tacggagcca tggacggcgc ctcgaagttc
     1981 gtgcagggcg acgtgaaggt ggggatcgtc atcacgctca tcaatatcat cggcggcttc
     2041 atcatcggca tggtgatccg cggcgagtcg ttcgacgtcg cgctcaagac ctacacgctg
     2101 ttgtccatcg gcgacggcct cgtggcgcag atcccctcgc tcctggtgac caccgcgacc
     2161 ggtatcatcg tgacccgggc cgtatcggac gataacttcg gcaccgacct gggcaaccaa
     2221 atcgccgcgc agccgcgcgc gctcctcatc accgccgggg cgctggcggc atcggccatc
     2281 atccccggct tccccaaaat ctccatcctc atgctcgcgg ccggcctggg cgcgctgggg
     2341 tacatcctcc agcagacgca ggaggaaacg cagaagaaag aggagatcga cgagaaggag
     2401 gcgcaactca aggagcacaa acccgaatcg gtcatgccgc tcatgcaggt ggacccgctc
     2461 gaggtggaga tcggctacag cctcatcccg ctggttgatc cggagcaggg cgggacgctt
     2521 ctggaccgga tcaccaacat ccgccgccgc tgtgcgctgg acatgggatt ggtggtgccg
     2581 cccattcgca ttcgcgacaa catggagctc gagccggact cgtacgccgt gctcatcaag
     2641 ggcgtcgaga tcgggcgggg gaacctgcag gtgggacgcc tgatggcgat ggatcccgga
     2701 ggcgtgaagg agcgcatcga gggcgacgag ttcgtggagc cggtcttcgg gctcacggcc
     2761 atctggatcg agccggactt gcgcaatacc gccgagagcg gcggctatac cgtcgtcgat
     2821 tgccccacga tcatcgcgac gcacctcacg gagatcatca agcggcacgc ggacgagatc
     2881 ctgggccgcc aggaagtgaa gcagatcgtg gacaacatca agagcgacta tccggcggtg
     2941 gtggacgagc tcctcaacga gaagaagatg tcgctgggcg aaatccagcg aattctccag
     3001 aacctgctgc gcgagcgcgt gtccatccgc aacatggtca cgatcctcga gacgctcgcc
     3061 agctatgtcg attacaccaa ggacgcgggc atgctcaccg agtacgtgcg caacgccctt
     3121 tcgcggcaga tctgcctcga gcatgttgac cgcgaaggga tgctctcggt gatcacggtg
     3181 agcccggaga tagagggcat catccgctcg tccatccacg acgatcccat cgaggggcgc
     3241 atcgtgggcc tggagcccga tgtgcacggg cgcatcgtgg ccgccttcat cgaggcgtac
     3301 aagaagtcga aggacgccgg gttcgcgccg gtgtaccttg tgtcgccgca gatccggagc
     3361 gtcaccttca cgctcctgga gcgcgagatc gcggacccgt tcgtgctggg gtacaacgag
     3421 gtccgcccgg aaattaaagt cagagtcatc tcgaccgcgt tgatgtcgac ggcggcctga
     3481 tcacgatata ctatttcggc aaggagtggg tcatgaggta cgtgaagatc aaggcgaaga
     3541 attacaatga ggccatgatg aagctcaaga tggagtacgg cgacgaggcc atccccatca
     3601 gccataaata cgtcaaggag ggcgggctct tcaatacggc gctcatggcc aaagatctcg
     3661 tggagctcac cgcggccatc caggagtcga agcccgctcg gggcctcggg gtcaagcccg
     3721 agaagaaatc gcacatcgac ttcaccgtgg gcgacaccac ggagacgaag cccctgtcgt
     3781 cggtcgcgcc gccgaaggcc gccgtcgaca acggcccgct gatggacgcg ctcaacgggc
     3841 tgtacgatcg cggcatggcg aaaacggaga cgaagagcgc ctcggtgggc gcgctccagg
     3901 acacgatccg caaggccgcg gccgtcgccg aaccgcgcgt cgcatcggcc gggtcagact
     3961 cgatcgcgaa gctggagaag gaattccagg acctcaagaa catgctcaac cgggtgatcg
     4021 aaaccaaggg cgcggcggcc gagaagtccg cagcggacac ggccgaggat gatcgcacca
     4081 tgcgtcccgt tctcgagata ctcaggaaca acgacttcga ccacgatgag tgcaccctca
     4141 tggtcgagga gatcaagaac aacgtgagtc gcgaggacct caaggaccgc tataagattg
     4201 aaaaaaccct gcgcgagctg ctcaagaacc gcatcgtgac cagcgggccc atcaccaccg
     4261 ggaaccggaa gaaggtgatc atgttcttcg gccccaccgg cgtgggcaag accaccacga
     4321 tggccaagct gggcgccatc cacgcgctgc gcgaggggca ccgcgtgacc ttcatcacca
     4381 tcgacaccta ccggatcgcc gcgaccgaac agcttaagaa atacgcggag atcatgaaga
     4441 tcccgctgcg cgtcgtgagc gaccagtcgg ggctcaagga ggtcctcgac aaggagaagg
     4501 ccgagatcat catgatcgac acgtcgggcc gcagccacaa gaacctgatg aagatctcgg
     4561 agatcaagag cttcgcggac g
//
LOCUS       DAORHF010000280         4575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20011_length_4575_cov_3.975885, whole genome shotgun sequence.
ACCESSION   DAORHF010000280 DAORHF010000000
VERSION     DAORHF010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20011_length_4575_cov_3.975885"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..288
                     /locus_tag="PKJ16_16635"
     tRNA            216..288
                     /locus_tag="PKJ16_16635"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Cys,seq:gca)
     gene            365..448
                     /locus_tag="PKJ16_16640"
     tRNA            365..448
                     /locus_tag="PKJ16_16640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:399..401,aa:Leu,seq:taa)
     gene            complement(455..1084)
                     /locus_tag="PKJ16_16645"
     CDS             complement(455..1084)
                     /locus_tag="PKJ16_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48673.1"
                     /translation="MKRIASIAAIIILSAVATGAHPHYRTYTNSPFNYKIIVPSEWER
                     VVLNLSKKHIMYASKSATVEIKVRAFRGDDVRFDNIVHDERWDLRKIDSHLNSIIETG
                     NIAIKKNVKGKLFVFEYTMHRTRMIQRTMITKNADTVYIIECRAPRKTFYTYEKIFTT
                     ALGSFANLEVEKTRSDDNLDDLEDEKTERKGGKKSSSSDDVLEADDERI"
     gene            complement(1133..1876)
                     /locus_tag="PKJ16_16650"
     CDS             complement(1133..1876)
                     /locus_tag="PKJ16_16650"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HNV48674.1"
                     /translation="MTKKAPKAYKDQQFLNSREARTLRIMAEYLEPEARLKKFNIQHT
                     VVFFGSARLKPDGRRTIDKAYWVAEELAYRLAVLSHEMELTGKNFCICTGGGPGIMEA
                     ANRGANRAHANTIGYNISLPMEQEPNDYLTPELNFEFHYFFMRKLWFMYHAKAVVVFP
                     GGFGTLDELFEVLMLVQTQKLNKRMIPILLYDKEFWDEIININKLVEHRLISREDRDL
                     IQFFSSADEGFEIIKSKLGKMIASVNHVK"
     gene            complement(1873..2457)
                     /locus_tag="PKJ16_16655"
     CDS             complement(1873..2457)
                     /locus_tag="PKJ16_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48675.1"
                     /translation="MTNEHEITFLDSFAADTIVVPQVIDNLINDLKRMSYPKDDIDEI
                     ILSMDEAITNAIQETVRKEETTRTADDNERRKITIRYSIREDNFDATIIDHGKGLDLS
                     RLKGFVPNIDSSNYYDQIVKYASESEKNRLTVRVNGKEITLKGIGVGLKVILAFMDSV
                     SIDLIDKERVLANSVSEFTDGTILNLNRRRRYTA"
     gene            complement(2749..>4575)
                     /locus_tag="PKJ16_16660"
     CDS             complement(2749..>4575)
                     /locus_tag="PKJ16_16660"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HNV48676.1"
                     /translation="FFPPLVSLLARERGSAAGGTAYAIESLGSFAGGLVFSIVFVDHI
                     NPIGITALLFCIGMMPCLYAHARAALYPLALLPLAIAAMSSPIESSLLAAMWNRTHAG
                     ALVEYHRTRHQAVQVESQDGQINIYGDGVFYHSLPDRHDARGLFHLIQSLRNGTGERV
                     LLFGTGPGSLPYNLGKSDVARVDYCEPDPALWEITDAYRRRFYPRNDGRLHVITADLR
                     RYFRENDVRYDIIVSRAAAPENVMLNRFHTKEYFEQCRAHLKPDGVFITAVHGFSNYL
                     SDDLLHFIASIYKGFRETFPHHRITSGDTMYLIGSPDPSRLPDDPASLIARYRAALEK
                     KPLTGLEDDIITNFVPDELLMSFEKTQINYFQDKIDRILPSVDANSDHRPLGYWNLVK
                     LSAYQEESAVYSIFRHAPIVPAIGCVLVALALIDARRRFPGVGFAANATMFATGCASM
                     AAMIVLMILYQNANGIIYYRISLMNALFMFGLTAGSWLATAHRLPSLPVRLILLMASL
                     GGILLYASRSVEPLFWLLIAAISLISGTIFPSLFSLSDNDGHHAFAASLDAMEHLGSI
                     VGAAATAMLLIPLLGMSSAIAACMCIVAFAALVLLVSGVRKA"
BASE COUNT          941 a         1185 c         1416 g         1033 t
ORIGIN      
        1 acctcgaacg acagaaggat tactctttga atcatggtcc atgtttataa gtggtaataa
       61 aatgtcaata tttaataagg ccataaaagc aaaacgaaat gtatttttat gtaaatattc
      121 acatcatgcc gaatggaatg gcgggcgtta caaaatcgat gatttttctg ttgacaaggg
      181 tagggccaaa ctcgatgatt gccatcgttt gaaatggcgc cgtacccaag tggctaaggg
      241 agaggtctgc aaaaccttta tgcagcggtt caaatccgct cggcgcctca tcgttttttc
      301 ccgccacgaa ggccgattgc tgcgaggtat cggttctaac cggataatgg ccgtaatcgc
      361 gaacgcccag gtggtgaaat tggtagacac aagggactta aaatccctcg gacctcggtc
      421 tgtgccggtt caagtccggc cctgggcacg ttaattatat ccgctcgtca tcggcctcaa
      481 gcacgtcgtc ggaggacgac gacttctttc cgccctttct ttctgttttt tcgtcttcca
      541 gatcgtccag attgtcgtcc gatcgggttt tttctacctc gagattggca aagcttccca
      601 gcgcggtcgt gaatattttt tcgtaggtgt agaacgtctt gcggggggcg cggcattcga
      661 tgatgtatac ggtatccgcg tttttcgtga tcatcgtgcg ttgaatcatg cgcgtccggt
      721 gcatggtgta ctcgaacacg aacagcttgc ccttcacgtt tttcttgatg gcgatattgc
      781 ccgtttcgat gatgctgttg agatgggagt cgatcttccg caggtcccag cgctcgtcgt
      841 ggacgatatt gtcgaaccgg acatcatcac cccgaaacgc ccgcaccttt atttcgaccg
      901 tcgcgctctt gctcgcgtac atgatgtgct tcttggagag attgagcacc acgcgctccc
      961 attcggaggg gacgataatt ttatagttga aggggctgtt ggtgtaggtc cggtaatgcg
     1021 ggtgggcccc cgtcgcgaca gcgctcagga tgatgatggc ggcgatgctg gcgatccgtt
     1081 tcataacgcg ttccttgtgt tggcgaagca tggtgtgtct ttgcgcatga tattacttga
     1141 cgtgattgac gctggcaatc atttttccga gcttggattt aataatttcg aacccctcat
     1201 cggcgctcga gaaaaactga atgaggtcgc ggtcctcgcg gctgatgagg cggtgttcca
     1261 cgagcttgtt gatgttgatg atctcgtccc agaactcctt gtcgtagagc aggatgggga
     1321 tcatgcgctt gttgagtttc tgcgtttgca cgagcatgag gacctcgaag agctcgtcga
     1381 gggtgccgaa cccgcccggg aacacgacca cggccttggc gtggtacatg aaccacagct
     1441 tccgcatgaa aaaataatga aactcgaaat tgagttccgg ggtcaggtag tcgttgggct
     1501 cctgctccat gggcagggat atgttgtagc cgatcgtgtt cgcgtgcgcc cgatttgcgc
     1561 cgcgattcgc cgcctccatg atccccggcc cgccgccggt gcagatgcaa aaattcttcc
     1621 cggtgagttc catctcgtgc gagaggaccg cgaggcgata tgcgagctcc tcggcaaccc
     1681 agtaggcctt gtcgatggtg cgccgcccat ctggcttgag ccgagccgac ccgaaaaaca
     1741 cgacagtgtg ctggatgttg aacttcttga ggcgcgcctc gggctcgaga tactcggcca
     1801 tgatgcgcag ggtgcgggcc tcccgcgagt tgaggaattg ctggtccttg taggccttgg
     1861 gcgccttttt ggtcatgcgg tataccgcct gcggcggttg agattgagga tggtcccgtc
     1921 ggtgaattcg gaaaccgagt tcgcgagcac ccgctccttg tcgatgaggt cgattgagac
     1981 ggagtccatg aaggccagta tcaccttgag gcccacgccg atccccttga gggtgatttc
     2041 cttgccgttg acgcgcaccg tgagccggtt cttctcggac tcgctggcgt acttgacgat
     2101 ctgatcgtag tagttcgacg agtcgatatt ggggacgaac cccttgagtc gcgaaaggtc
     2161 gagtcctttt ccgtggtcga tgatggtcgc gtcgaaatta tcctcgcgta tgctgtagcg
     2221 tatggtgatc ttgcggcgct cattgtcgtc ggcggtgcgg gtggtctcct ccttgcgcac
     2281 ggtctcctgg atggcgttgg tgatggcctc gtccatcgac aggatgatct cgtcgatgtc
     2341 gtccttggga tagctcatgc gcttgaggtc gttgatgaga ttgtcaatga cctgcggcac
     2401 gacgatcgtg tcggccgcaa agctgtctag aaatgtaatc tcgtgttcgt tggtcatgcc
     2461 gatacagccg cgtggcgcag gaaacgtcgc cggggaccgg cgtgcgcctc atatcgcacc
     2521 ttggtgctcc gtgcggaggt gttgcgcaat catattccac agcgagtttt tttttcaagt
     2581 agtttttatg gcgtcacggg gtattgtcca agtattattg aaagaatcca atatggcgga
     2641 gcgggcggtg ccggttggcc cctgggggaa tgcggatcgg ggcgatttcg catacgtccg
     2701 tccatgactg tatgtcgaac gaaggtttcg aagtcgagaa aaacacgatc aggctttccg
     2761 tacgcccgat accagcagga ccagcgcggc gaatgcgacg atgcacatgc aggctgcaat
     2821 ggcgctggac atgccgagga gcgggatcag aagcatcgca gtcgcggctg cgcccacaat
     2881 cgaaccgaga tgctccatgg cgtccagcga tgcggcgaag gcatgatgcc cgtcattgtc
     2941 cgagagcgaa aacaggctcg gaaagatggt gccgcttatg agtgagattg cggcgatgag
     3001 cagccagaag agcggctcca cgctccggga cgcgtacagg aggatcccac ccagcgatgc
     3061 catgagcaga atcagccgta cgggcagcga cgggagccga tgcgcggtcg cgagccagct
     3121 gccggcggtc aatccgaaca tgaacagcgc gttcatcagg gagatgcggt aatagatgat
     3181 cccgttggcg ttctgataca gaatcatcag cacgatcatg gccgccatgc tggcgcagcc
     3241 ggtcgcgaac atcgtcgcat tggcggcgaa cccgactccg gggaagcggc gtctcgcgtc
     3301 gatgagcgcg agcgcgacga gcacgcagcc gatcgcagga acaatcggcg catgtcggaa
     3361 gattgaatac acggcggatt cctcctggta ggccgaaagc ttcaccaggt tccagtagcc
     3421 gagtggtcga tgatcgctat tcgcgtctac cgacggaagg atcctatcaa tcttatcctg
     3481 aaaataattg atctgtgttt tttcaaatga catgagcagc tcatcgggga cgaagttcgt
     3541 tatgatgtca tcctcgagac ccgtaagcgg ctttttctcc agagcggcac gatagcgcgc
     3601 gataagcgat gcgggatcat ccggcagccg cgacgggtcc ggcgatccga tgagatacat
     3661 cgtatcgccg gaggtgatgc ggtgatgggg gaaggtctcc cggaacccct tgtatattga
     3721 ggcaatgaaa tgaagcaaat cgtcggacag gtaattggag aacccgtgca cggcggtgat
     3781 gaaaacgccg tccggtttca agtgcgcgcg gcattgctcg aagtattctt tcgtatggaa
     3841 tcgattgagc atcacgtttt ccggggccgc ggcgcggctc acgatgatgt cgtaacggac
     3901 gtcgttctcc cggaaatacc gtcgcagatc ggcagtgatg acatggagcc ttccatcgtt
     3961 acggggatag aaacgacgac gataggcgtc ggtgatctcc cagagcgccg ggtccggttc
     4021 gcagtaatcg acacgcgcca catcggattt cccgagattg tacggcagtg agcccggccc
     4081 ggtcccgaag aggaggacgc gctcgccggt cccgttgcga aggctttgaa tcaggtggaa
     4141 gaggccgcgc gcatcgtgcc ggtcgggcag ggagtgataa aacacgccgt cgccgtagat
     4201 gtttatctgc ccgtcctggg attccacctg tacggcctgg tggcgcgtcc ggtggtattc
     4261 gaccaacgcg cctgcgtggg tccggttcca catggcggca aggagcgatg attcgatggg
     4321 ggacgacatg gcggcgatcg cgagcgggag cagggcgagc ggatagagcg ccgcgcgtgc
     4381 atgcgcgtac agacacggca tcatcccaat acaaaaaaga agcgcggtga ttccgatggg
     4441 attgatgtgg tccacgaaca cgatcgagaa gacgagcccg ccggcgaatg agcccagcga
     4501 ctcgatggcg tatgcggtgc cgccggcggc tgaaccccgt tcgcgggcga ggagggacac
     4561 cagcggcggg aagaa
//
LOCUS       DAORHF010000281         4568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20048_length_4568_cov_6.093508, whole genome shotgun sequence.
ACCESSION   DAORHF010000281 DAORHF010000000
VERSION     DAORHF010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20048_length_4568_cov_6.093508"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PKJ16_16665"
     CDS             <1..496
                     /locus_tag="PKJ16_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48677.1"
                     /translation="TKLVRWAAERAGDQRMEFFGALPAVRLADEQRRERMCTAGYLRD
                     ALAASLLLYPVAEPLAIGGTRYHAGYPDCMARGADLRRAGIDEVLLVSIASRSRWDFS
                     DGRLLSFYGTYLNALHGRRSESDRADAIITIDIDEKRYKPAKIISDAEKAVAGALAKS
                     HNPS"
     gene            557..1003
                     /locus_tag="PKJ16_16670"
     CDS             557..1003
                     /locus_tag="PKJ16_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48678.1"
                     /translation="MRSRATNLLQAAALFAGIVYLVVGSLFYLSPILFSRICGMDVGE
                     DWLTEIPKDPLMSTLYYMATAFASLLGVTGASMVLPLFDPEKYRVLMYFTGAIFPGFA
                     AVTLLRNGIRLGHPLLIVFGGVFLAICVVTVLGIAATRHAGRPGGR"
     gene            1220..1564
                     /locus_tag="PKJ16_16675"
     CDS             1220..1564
                     /locus_tag="PKJ16_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48679.1"
                     /translation="MDLKTKKVDNVIVVHLFGRLDVHLSADIEKEIGKIISDEPSSHL
                     LLNLEDVEYMSSSGLRIFVSTMRILKESKRKLKLCNIKSAVKKIFEVVELIDMFDIYD
                     SEEAALQAFKSE"
     gene            1572..3842
                     /locus_tag="PKJ16_16680"
     CDS             1572..3842
                     /locus_tag="PKJ16_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48680.1"
                     /translation="MPLPWISTKNEPADASINLNDIKADFVDTAKIAAGRSDYLRDLR
                     NYVLFQKMPTFRWPLWLTGLADPASEYHRPIVPAFLSNTAGTDAIAFSNILSSDDLLV
                     DLAGMLSPTYATWSMEVWMIADGALIRPQERPGRVSMERIPRRGMVKAVWDESAFRVT
                     TELFGIRTDIDEAVIDIACSVKKSVQTAVLCVAFRPYNGSTLGGIEAIEYQGDTRIVR
                     INGRERALVGAKPDAVAAGSFDAGDIDAASAAGGEHKARCRYGMATLGLFYRLSRGEN
                     ALTVRLSLARDRALRPAKLDFKKLREDFSAFANQRANAGVSIAIGDRGIEQWFLASKL
                     SLLSHLNNGSLFPGKSMPDVAQARLLYYVLAAYVRMGAFAETLMVLEAFLGSLSLGER
                     PGFHDIVVACYFLCAAGDYFTVSRNMDHLKTVFAAIRAVAVPVLAHAAGIKEKQVRSA
                     DAVNTIPYSFLRHAHLHDIFLAACACRQFAYLARCMGLFGDELKFAKESQRLEEIALR
                     EIAQRFPAPRAGKKDDAAARRAAIASEDETLIYCLFAGFPFGLTLLSPEKLRTMTDAL
                     VERYGGVPLYLRSRGGWDSFLSLALAHNLIALKDARAFDIITHFFDAGGKRFSVPDVI
                     NPKSRHGVFGEGDSRLAHAVLFLALRNLLFVDAENRLEIFPLPREEWFTPGSEIVIRD
                     APSRFGLINLRAVSTPNEIQVHFEQLPQFLPHDMLITLPVKTKLVKEDDFIIKKELGN
                     AYLISGWPTIVRFLRQ"
     gene            3861..>4568
                     /locus_tag="PKJ16_16685"
     CDS             3861..>4568
                     /locus_tag="PKJ16_16685"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48681.1"
                     /translation="MIVYILLAAVIFLGLLIYYLFYIAPRFNPRNRAASFEQQGLTRE
                     AIYEYKKILDENPRDPAVHFKLAGLFLSVDEPDKAALHLEEVMKIGQYSAEVDKLDTM
                     KRLAGIYQDREDLERAFQVYFELLRTYPADEEALYHVSFIALGQEEFELAQRYFDRLV
                     KLKPGDFDIQFGAGICSYQNQKINESVEYFKAAVALRTNSDLANLAMAFALQRKRDYK
                     QAMAVLGKLAATTPDPQV"
BASE COUNT          823 a         1409 c         1402 g          934 t
ORIGIN      
        1 cacgaagctg gtcagatggg cggccgagcg cgcgggcgat cagcgaatgg agttcttcgg
       61 cgcgctcccg gcggtccggt tggccgacga acagcgacgg gaacgcatgt gtaccgcggg
      121 atatctgcgg gacgcgctcg ccgcatcgct tctgctgtat ccggtggccg agccgctcgc
      181 catcggcggc acgcgctatc atgccgggta tccggactgc atggcgcgcg gtgccgatct
      241 gcgccgcgcc ggcatcgacg aggtactgct agtgtcgatc gcgagtcgct cgcgctggga
      301 tttttcggac gggagactcc tttcctttta tggaacgtat ctcaacgcgc tccacgggcg
      361 gcgttccgag agcgatcgag ccgatgcaat catcaccatc gacattgacg aaaagcgcta
      421 caagccggcc aagatcatct ccgatgcgga gaaggccgtc gccggcgccc tcgccaaaag
      481 ccataatcct tcttgacttt ctcctcgcct gatcgtatct gtcgctaccg gacatgacaa
      541 tcgatagggg ggcgtgatgc gatctcgtgc tacaaatctg ctgcaggcgg ccgcgctgtt
      601 cgcggggatc gtgtatctgg tcgtggggtc gctgttctac ctgtctccca tattgttcag
      661 ccggatatgc ggcatggatg tgggcgagga ctggctcacc gagattccca aggacccgtt
      721 gatgtcgacc ctgtattaca tggcgacagc cttcgcctcg cttctgggcg tgaccggcgc
      781 gtcgatggtg ttgccgctgt tcgatcccga gaagtatcgc gtgctcatgt actttacggg
      841 ggcgatcttt ccgggcttcg cggcggtcac gctgcttcgt aacggcatcc ggctggggca
      901 cccgctcctg atcgtgttcg gcggcgtgtt tctcgcgata tgcgtcgtga cggtgctcgg
      961 cattgcggcg acccggcacg cggggcgtcc gggcgggcgg taaaccggga tcgtccggcg
     1021 gtaatattaa cagcggtcaa atagtgtaaa gaatattggc ggaaaataat acaacaatag
     1081 cttgacaaaa accgggtgtt cgaagataaa ttcacattga gagcatagtg gccgttccgg
     1141 catgtggagc gtgtaacgct tttaccggag tatctttttt tagcaatgga atgcacccga
     1201 gcgcaataag gaggggttga tggatctcaa gacgaaaaag gtggacaatg tcatcgtcgt
     1261 gcatctcttc gggcgcttgg acgttcactt gtcggccgac atcgaaaagg aaatcggcaa
     1321 gatcatatcg gacgagccct ccagccatct gcttctcaac ctcgaagacg tcgagtacat
     1381 gagcagctcc ggattgcgca tcttcgtgtc caccatgcgc attctcaagg aatcgaagcg
     1441 caaactcaaa ctgtgcaata tcaaaagcgc ggtgaagaag attttcgagg tcgtcgagct
     1501 tatcgacatg ttcgatattt acgattccga agaggcggcg ctgcaggctt tcaagagcga
     1561 gtagcccgga cgtgccgctt ccctggatca gcacgaaaaa cgaaccggcc gacgcgtcca
     1621 tcaatctcaa tgatataaag gcggattttg tcgatacggc aaaaatcgcc gccggccgca
     1681 gcgactatct gcgggacctc cgcaattacg ttctctttca gaaaatgccc acgttccgtt
     1741 ggccgctctg gcttacgggg ctcgccgatc cggcctccga gtatcatcgt cccatcgtcc
     1801 ccgccttttt gtcgaatacg gccgggaccg acgcgatcgc cttctccaat atcctttcct
     1861 cggacgacct attggtcgat ctggccggga tgctctcgcc gacctatgcg acctggtcga
     1921 tggaggtgtg gatgatcgcc gacggcgcgc tcatacgccc gcaggagcgc cccgggcgcg
     1981 tctcgatgga gcgcattccg cgccgcggca tggtgaaggc cgtctgggac gaaagcgcct
     2041 tccgggtgac gacggagctc ttcggcattc gcacggacat cgacgaggcg gtgatcgaca
     2101 tcgcatgctc cgtgaagaag tccgttcaga cggccgtcct gtgcgtcgcg tttcgtccgt
     2161 acaacgggag caccctcggc ggcatcgagg cgatcgagta ccagggcgac acgcgcatcg
     2221 tgcgcatcaa cgggcgcgag cgggccctcg tcggggccaa gcccgacgcc gtggccgcgg
     2281 gcagcttcga tgcgggcgat atcgacgccg caagcgcggc cgggggagag cacaaggcgc
     2341 gctgccgtta cgggatggcc accctggggc ttttctaccg cctttcgcgc ggcgagaacg
     2401 cgctcaccgt gcgcttgagc cttgcgcggg accgcgcgct tcgcccggcc aagctcgatt
     2461 tcaaaaaact ccgcgaggat ttttcggctt ttgcaaatca acgggcgaat gcgggcgttt
     2521 cgatcgccat cggcgatcgg ggcatcgagc agtggtttct cgcctccaag ctctcgctgc
     2581 tgtcgcacct gaacaacgga tcattgttcc cggggaaatc catgccggac gttgcgcagg
     2641 cgcgactgtt gtattatgtc cttgccgcct acgtgcgcat gggcgccttc gccgagaccc
     2701 tcatggtgct ggaggccttt ctcggttcgc tctcgctggg cgagcgcccc ggttttcacg
     2761 atatcgtggt cgcctgctat ttcctctgcg ccgcgggaga ctatttcacc gtttcgcgca
     2821 atatggacca tctcaagacg gtctttgcgg ccattcgcgc ggtcgccgtc ccggtgctcg
     2881 cccatgcggc agggatcaag gagaagcagg tccgatccgc ggacgcggtc aacaccatcc
     2941 cctacagctt tctccgccat gcgcacctgc acgatatatt cctggcggcg tgcgcctgcc
     3001 ggcagttcgc ctatctcgcg cgctgcatgg ggcttttcgg cgacgaattg aagttcgcga
     3061 aggagtccca gcggctcgag gagatcgcgt tgcgcgagat cgcacagcgg ttccccgcgc
     3121 cgcgcgccgg gaagaaggac gacgccgccg cgcgccgggc cgcgatcgcc tccgaggacg
     3181 agacgctcat ctattgcctg ttcgcggggt ttcccttcgg gctcaccctg ctctctccgg
     3241 agaagctgcg caccatgacc gacgcgctcg tcgagcgcta cggcggcgtc ccgctgtatc
     3301 tgcggtcgcg gggcggctgg gactcgttcc tctcgctggc gctggcgcac aatctcatcg
     3361 cgctcaagga cgcgcgggcc ttcgacatca tcacgcactt cttcgatgcg ggaggaaagc
     3421 gcttctcggt cccggacgtg atcaacccga agagccgtca tggcgttttc ggcgagggcg
     3481 attcgcgcct tgcgcatgcg gtgctctttc tcgcgcttcg taacctgctg ttcgtggacg
     3541 ccgagaaccg gctcgagatc tttcccctgc cccgggagga gtggttcacg ccgggttccg
     3601 agatcgtgat ccgcgacgcg ccgtcgcgtt tcggcctcat caatctccgg gcggtctcga
     3661 cgccgaacga gatccaggtc catttcgaac agctgccgca gttccttccc cacgacatgc
     3721 tcatcaccct tccggtgaag acaaagctgg tcaaggagga cgatttcatc atcaaaaagg
     3781 agctcggcaa tgcctatctc attagcggct ggccaaccat cgtcaggttc ctgcgccagt
     3841 gagccgccgg gtgcgtccag atgatcgtct acatcctgct cgccgccgtc attttcctgg
     3901 ggctgctcat ctattatctg ttctatatcg cgccccggtt caatccgcgt aatcgcgcgg
     3961 cgtcgttcga acagcagggg ctcacccgcg aggcgatcta cgagtacaag aagatcctcg
     4021 acgagaaccc gcgcgacccg gcggtccact tcaaactcgc cggcctcttt ctgtcggtcg
     4081 acgagcccga caaggccgca ctgcacctcg aggaggtgat gaagatcggc cagtactcgg
     4141 ccgaggtgga caagctggac acgatgaagc gcctggcggg tatctatcag gaccgtgaag
     4201 atctcgagcg ggccttccag gtgtatttcg agcttctgcg cacgtatccg gccgacgagg
     4261 aggcgctgta tcacgtgtcg ttcatcgcgc tggggcagga ggagttcgag ctggcccagc
     4321 gctatttcga ccgtttggtg aagctcaagc cgggcgattt cgacatccag ttcggcgccg
     4381 gcatctgcag ctaccagaac cagaagatca acgagtcggt cgagtatttc aaggcggccg
     4441 tcgcgctgcg caccaactcc gatctcgcca acctggcgat ggcctttgcg ctccagcgga
     4501 agcgcgacta caaacaggcg atggccgtcc tcgggaagct tgccgccacg acgccggacc
     4561 cgcaggtg
//
LOCUS       DAORHF010000282         4544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20196_length_4544_cov_5.402985, whole genome shotgun sequence.
ACCESSION   DAORHF010000282 DAORHF010000000
VERSION     DAORHF010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20196_length_4544_cov_5.402985"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PKJ16_16690"
     CDS             <1..598
                     /locus_tag="PKJ16_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007016155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV48682.1"
                     /translation="AARAGEQGRGFAVVASEIRNLAQRSGNAAKEIADMIKDTVEKVS
                     NGTELVNNSGEALKEIIKSIKDLGRFISEIAASSEEQMKGIDQINVAVSEMDTMTQQN
                     AALVEETASASEEMSNQAQELLAMTEKFIISDGLRKETAQKKHKELHLHGAETVAPNK
                     KKVARLDHKGGNGGKADAGATKDSDIKRAMESEGFEEF"
     gene            734..895
                     /locus_tag="PKJ16_16695"
     CDS             734..895
                     /locus_tag="PKJ16_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48683.1"
                     /translation="MNDGRDMDDRLLQLREREEKPAAPEPAWLQGRREYQWFHENRTR
                     IEEMIKVLQ"
     gene            1033..3129
                     /locus_tag="PKJ16_16700"
     CDS             1033..3129
                     /locus_tag="PKJ16_16700"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HNV48684.1"
                     /translation="MIDATVREVFIQEAQEILKKLESDVVRLEERFDHDILHDVFRSF
                     HTLKGSSGIVGMTDLSEFTHHLENMMASVRDGKTDPGRGMIDIILNSIDWIKLYLFGD
                     SGAEALSDIRGKLERAIAEYGVAGPSEKGAPAAEAPAEDGVLYRYFKVAARFPHNVFE
                     KGIDPLMIIEDLCALGTVVERAVNRDALPEFGAMDPEKCYLSWEVLLKTEKTRKDVDD
                     VFLFVSEESEIAVADVTAECITLDGGALSRERRLGEIMVNKGIITDGQLLDAVHEQEE
                     RNVRLGDLVVQKGYATPDDVRIALTEQEKLKTRIETDTVRVDTAKLDRVMNLLGEIVI
                     GQAAITRVADLLSEQHSYQLKSALYGLDRTTRELQEQIMSIRMIPIGPTFDQFRRFVR
                     DTAHALGKEIVLEMSGEDTELDKTVIEKIGDPLKHMIRNAIDHGIEPAAERELAGKPR
                     AGRIRLRAYHQEGNVFIEVVDDGRGLDYPRMREKAIMMGLVRADEEVGESRLASFLFL
                     PGFSTAKSVGDLSGRGVGMDVVKTNIDALRGVVTVESRKNAGCTVRVKLPLTLAIIEG
                     MLVRVGAQVYIIPLLSIVESLRPRVEDVKTVEGAGEVISVRGEYIAMVRMDRFFGIEA
                     DIANPWEALVVIVEASGSKIGLMVDDLLGQHQVVIKSMDGGVTASRAISGASILGDGK
                     VALIIDTHGLAGELAS"
     gene            3164..>4544
                     /locus_tag="PKJ16_16705"
     CDS             3164..>4544
                     /locus_tag="PKJ16_16705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48685.1"
                     /translation="MNAQANFALYSVFELMRDDRERLADLKPRLSTIFQEIGDFLLGT
                     SRELDSFRGIIGPYMEYFYPAGAAPANDAGSFVADVVLPSRRTMDEAIDKAVRIMQND
                     AAINDAVAGAIEQSLAIRDKIDTMIDLIETIEIYADNARLVSIKAGAEGQTLARISEE
                     MSALSGVANGLSGDSRALIGKMDEAYTRFRKIRERIDILNENYLTQMKLKCRIVFGDL
                     QRDLDALSGAARGMMDYSADIERSIGSSLGCLQVEDLIRQDIERIIALVEESCALERD
                     EDAGESYRPIVLAVMRDRMNGVFSNADMLVMETHDCCARMKDILNRFIGSIGTDDALG
                     DGSEARFGHVYGRLERIRDEFVGYIVEIIERKKTLYAVAAEILEIMDGFGTSLQGMTS
                     VIRRFEIINMLTKIELAKNFELRKTLATSLTDVADLPGKMKRIVGDTSGVYAALRENM
                     RASIEEYRVN"
BASE COUNT          903 a         1341 c         1604 g          696 t
ORIGIN      
        1 agccgcgcgc gcgggcgagc aggggcgcgg ttttgcggtg gtcgctagcg agatcagaaa
       61 cctcgcgcag cgctccggca acgccgccaa ggagatcgcg gacatgatca aggacacggt
      121 ggagaaggtg tccaacggga ccgagctggt gaacaacagc ggcgaggcgc tcaaggagat
      181 catcaagtcc atcaaggacc tggggcgctt cattagcgag atcgcggcct ccagcgagga
      241 gcagatgaag gggatcgacc agatcaacgt ggccgtctcg gagatggaca ccatgaccca
      301 gcagaatgcg gcgctggtgg aggagacggc ctcggccagc gaggagatgt ccaatcaggc
      361 gcaggagctg ttggccatga ccgagaagtt catcatcagc gacggcctgc ggaaggagac
      421 ggcgcagaag aagcacaagg agctgcacct gcacggcgcg gagacggtag cgcccaacaa
      481 gaagaaggtc gcccggctcg atcacaaggg cggcaacggc ggcaaggcgg acgcgggcgc
      541 cacgaaggat tccgatatca agagggcgat ggagagcgag gggttcgagg agttttagac
      601 cacctcacct gccctccggg ccgccctctc ccacaggaga gggctcatcc ggaaagggca
      661 cgttgccaga acgccccctt ctcctatggg agaaggggga cggggggatg aggtagagct
      721 gatgggagga gctatgaacg atggcaggga catggacgat cggcttctcc agctccgtga
      781 gcgcgaagag aagccggcgg cgcccgagcc cgcatggctc caggggcggc gcgagtacca
      841 gtggttccac gagaaccgta cccgcatcga ggagatgata aaggtgctgc aatgatcggg
      901 gagtaacctc acccccggcc cctctcccat aggagagggg tgtgcaggga aaagactatt
      961 tgcagtgccc cccctctccg atgggagagg gggtccgggg ggtgaggttg gatgaggtaa
     1021 ggaggaacgg tcatgataga cgcgacggtc cgtgaagttt tcatccagga ggcgcaggag
     1081 attctcaaga agctcgagtc ggacgtggtg cgcctcgagg agcgcttcga tcacgacatc
     1141 ctgcacgacg tgttccgttc gtttcacacc ctcaagggga gctccggcat cgtggggatg
     1201 acggatcttt cggagttcac gcaccatctg gaaaacatga tggcctccgt tcgcgacggc
     1261 aagacggacc ccggccgcgg catgatcgac atcatcctca acagcatcga ctggatcaag
     1321 ctctatctct tcggcgacag cggcgcggag gcgttgtccg atattcgcgg gaagctcgag
     1381 cgcgccattg cggagtacgg cgtcgcgggg ccgtccgaga agggcgcgcc ggcggcggag
     1441 gcccccgcgg aggacggcgt gctctatcgg tacttcaagg tggcggcgcg ctttccgcac
     1501 aacgtcttcg agaaggggat cgacccgctc atgatcatcg aggacctctg cgcgctgggg
     1561 acggtcgtgg agcgtgcggt gaaccgcgac gcgctccccg aatttggcgc gatggacccg
     1621 gagaaatgct acctgtcgtg ggaggtgctt cttaaaaccg agaagacccg gaaggacgtc
     1681 gacgacgtgt tcctgttcgt gagcgaggag agcgagatcg ccgtcgccga cgtgacggcc
     1741 gagtgcatca ccctcgacgg gggcgcgctc tcgcgggagc gccggctcgg ggagatcatg
     1801 gtgaacaagg gcatcatcac cgacggacag ctcctggacg ccgtgcacga gcaggaggag
     1861 cgcaacgtac ggctcgggga cctcgtggtg cagaagggct acgccacgcc cgacgacgtg
     1921 cgcatcgcgc tcaccgagca ggagaagctc aagacgcgca tcgagaccga cacggtgcgc
     1981 gtggacacgg ccaagctgga ccgcgtgatg aacctgctgg gcgagatcgt gatcggccag
     2041 gcggccatca cgcgcgtggc cgacctgctc tcggagcagc acagctatca gttgaagagc
     2101 gcgctctacg ggctggaccg caccacgcgc gagctccagg agcagatcat gtccatccgc
     2161 atgatcccca tcgggcccac cttcgaccag ttccgccgat tcgtgcgcga cacggcgcac
     2221 gcgctgggca aggagatcgt cctggagatg agcggcgagg acacggagct cgacaagacg
     2281 gtgatcgaga agatcggcga tccgctcaag cacatgatcc gcaacgcgat cgaccacggc
     2341 atcgagcccg ccgccgagcg ggagctggcg ggcaagccgc gcgccgggcg catccggctg
     2401 cgggcctatc accaggaggg gaacgtcttt atcgaggtgg tcgacgacgg ccgggggctg
     2461 gactatcctc gcatgcgcga gaaggcgatc atgatgggac ttgtccgggc cgacgaggag
     2521 gtcggggaat cgcgcctcgc gtcgttcctg ttcctgccgg gcttctccac cgcgaagagc
     2581 gtgggcgacc tctccggtcg cggcgtgggc atggacgtgg tgaagaccaa tatcgacgcc
     2641 ctgcgcggcg tggtcacggt ggagagccgc aagaacgcgg gatgcaccgt ccgcgtgaag
     2701 ctgccgctca cgctggccat catcgagggg atgctggtgc gggtgggggc gcaggtgtac
     2761 atcattccgc tgctttcgat cgtggagtcc ctccggccgc gcgtcgagga cgtgaagacg
     2821 gtggagggcg cgggcgaggt gatatcggtt cgcggggaat acatcgcgat ggtgcgcatg
     2881 gaccgctttt tcgggatcga ggccgatatc gccaatccct gggaggcgct ggtggtgatc
     2941 gtggaggcct ccgggtcgaa gatcgggctc atggtggacg acctcttggg acagcaccag
     3001 gtggtgatca agagcatgga cggcggcgtg acggcgagcc gcgcgatatc cggcgcctcc
     3061 atcctgggcg acggcaaggt ggcgctcatc atcgacacgc acgggttggc gggggagctt
     3121 gcgtcgtgac cctgcccggc ggacacataa aaggaaggaa tgtatgaatg cgcaggcgaa
     3181 ctttgctctg tactcggtct tcgagctcat gcgcgacgac cgcgagcgtc tggccgatct
     3241 caagccgcgg ctctcgacca tcttccagga gatcggggac tttctcctgg ggacctcgcg
     3301 ggagctggat tcgttccggg gcatcatcgg cccctacatg gagtatttct acccggcggg
     3361 cgccgcgccg gcgaatgacg ccgggagctt cgtcgccgac gtggtgctcc cgagccggcg
     3421 caccatggac gaggccatcg acaaggccgt gcgcatcatg caaaacgacg cggccatcaa
     3481 cgacgcggtc gcgggcgcca tcgagcagtc gctggccata cgggacaaga tcgacaccat
     3541 gatcgacctc atcgagacca tcgagatcta cgcggacaat gcgcgcctgg tgtccatcaa
     3601 ggcgggcgcc gagggacaga cgctcgcgcg catctccgag gagatgagcg cgctctccgg
     3661 cgtggcgaac ggactgtccg gcgacagccg cgcgctcatc gggaagatgg acgaggccta
     3721 cacgcgcttc cggaagatcc gggagcggat cgacatcctc aacgagaact acctgacgca
     3781 gatgaagctc aagtgccgca tcgtcttcgg cgacctgcag cgggacctgg acgcgctctc
     3841 gggcgccgcg cgcgggatga tggactactc ggccgacatc gagcgctcca tcggatcgtc
     3901 gctgggctgc ctgcaggtgg aggacctcat acggcaggac atcgagcgca tcatcgccct
     3961 ggtggaggag tcgtgcgcgc tggagcgcga cgaggacgcg ggcgaatcct accggcccat
     4021 cgtgctggcg gtgatgcgcg accggatgaa cggcgtattc tcgaacgcgg acatgctggt
     4081 catggagacc cacgactgtt gcgcgcggat gaaggacatc ctcaaccgct tcatcggctc
     4141 catcgggacc gacgatgcgc tgggcgacgg ctcggaggcg cgcttcgggc acgtctacgg
     4201 gcggctcgag cgcatccgcg acgagttcgt ggggtatatc gtggagatca tcgagcggaa
     4261 gaaaacgctc tatgcggtcg ccgcggagat cctggagatc atggacggat tcgggacatc
     4321 gctccagggc atgacgtcgg tcatccggcg cttcgagatc atcaacatgc tcacgaaaat
     4381 cgagcttgcg aaaaacttcg aactgcggaa gaccctggcc acatcgctca ccgacgtggc
     4441 cgatctcccc gggaagatga agcggatcgt gggggacacc tccggcgtgt acgccgcgct
     4501 ccgggagaac atgcgcgcct ccatcgagga ataccgcgtg aact
//
LOCUS       DAORHF010000283         4499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20498_length_4499_cov_5.240099, whole genome shotgun sequence.
ACCESSION   DAORHF010000283 DAORHF010000000
VERSION     DAORHF010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20498_length_4499_cov_5.240099"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..716
                     /locus_tag="PKJ16_16710"
     CDS             264..716
                     /locus_tag="PKJ16_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48686.1"
                     /translation="MIIAICIVVLLLSFGFHLFFLMQYVVTKDTRQLRRFVNTAISNI
                     VIAGGVTILVLFRPDLIRSIDVDMLLWMITGAIMVMMVTIKVTIVRNIMQRAKDPAFF
                     HYNYFGKKILDPGVVKPGEVMVFMLTMPFFLLAGAYFVARIVNWALKI"
     gene            736..1194
                     /locus_tag="PKJ16_16715"
     CDS             736..1194
                     /locus_tag="PKJ16_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48687.1"
                     /translation="MLISIFLVLMLLSFALHILFLGQYVMTRDNRALKRFVNTAVSNI
                     TIAGAMTVLVLFNPSLVRDIDVMFLLWLLSGGVMLILLGVKVMIFRTIYRRSQMPEHY
                     HFNFFGKKVLHATVVKPIEVMVFMITIPFFLICGAYFVARLINLFLYNRL"
     gene            1610..2305
                     /gene="queC"
                     /locus_tag="PKJ16_16720"
     CDS             1610..2305
                     /gene="queC"
                     /locus_tag="PKJ16_16720"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004925609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HNV48688.1"
                     /translation="MSKTAVVLLSGGIDSATVAMMAVRQGFSVHAITFSYGQRHAIEI
                     DCARRVAAQIGIAGHIVIDLNHAVFASSSLVAESGIPVPHGRDYAHSADIPSTYVPAR
                     NILFLSYALAYGETVGAHDIFIGVNAIDYSGYPDCRPEFIEAFARMADLGTRSGAEGR
                     GFVIHAPLVDMTKAEIIAHGHALGVDYSLTHSCYDPDREGRSCGTCDSCRIRRAGFSD
                     AHLADPTRYRSAS"
     gene            2302..3060
                     /locus_tag="PKJ16_16725"
     CDS             2302..3060
                     /locus_tag="PKJ16_16725"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HNV48689.1"
                     /translation="MTAGRGVIRRAAYALLCGAIDTVFPSRCAVCGASVSWGRHSLCA
                     ACHDTIPRAGDRCPRCSGYLDSGACSVCGTRAWYVDENIVVANYRGAMRTAVRKYKFS
                     GARRLARPFAELAAQRITDAEGATTCDIVTAVPMGRTKRWRRGYNQSEEVARLVARRL
                     GVPYRRLLRENRGALVQKTLGYRERFLNILGRFSVSPRRMPEGARVLVVDDIFTTGAT
                     INECARVLKGAGARAVYGCALARAAAHGDAERDA"
     gene            3092..3427
                     /locus_tag="PKJ16_16730"
     CDS             3092..3427
                     /locus_tag="PKJ16_16730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48690.1"
                     /translation="MIKVISDDSVEIVQLDGRIDQEASEELEGILEGLLGKKKFNICI
                     DMINVKHICSSALGVMVAIKRKIETNDGDIKLVIVNDNLLKLFQTTMLDKVFEIFETQ
                     RECLTAFID"
     gene            3469..4455
                     /locus_tag="PKJ16_16735"
     CDS             3469..4455
                     /locus_tag="PKJ16_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV48691.1"
                     /translation="MYTTLKSGLDDILRPIAAHLERVDAAIGEQLRTGVPLIDESSIH
                     LFTNKGKMIRASLIILASGMRGRIPEGIIDIAAAAEIVHAATLIHDDIIDNAEMRRGD
                     VAVSRRWGNKVAVLVGDHMYTRALEVAVRDDNYDLFPELVRATADMVKGELLQIEYSN
                     MERITAERYFEIIELKTARFLGACAKLGAIKAGLPAAEADALKRFGVHLGFAFQIVDD
                     TLDCIGDERSTGKDSGNDYLDAKATLPLINALEAASGPGRDELVRMCAQPAPEHWPAV
                     REAIRVSGALARCLDIAREYARRAVGEIDGFPDSECKRIILRLSDFLLERAY"
BASE COUNT          852 a         1288 c         1435 g          924 t
ORIGIN      
        1 ggccgccgca ccacgggcag gcccgcagga tctcgccgca ttcgacgcat cggtgcagcg
       61 cgaatccggg aggatcgtcc cggaggtgtc gcaccgatcg cgtgccgcat cgctcgcaga
      121 gcgccccgtc ccgcatctcg tccatcgata cgcctccccg ctgaaccgta aaaaaacttt
      181 acttgcaatt tttcgtcaag tcgtatacca tattaaaaaa tgtcattttt cgcgactata
      241 ctgggtaacg aaccgcgcac gcgatgataa tagcgatttg tattgttgta ttgctgctct
      301 ccttcggctt ccacctgttt tttctcatgc agtacgtggt caccaaggac acgcggcagc
      361 tcaggcgctt cgtcaacacg gccatcagca atatcgtcat tgcgggcggg gtgaccatcc
      421 tggtgctctt ccggcccgac ctcatccgca gcatcgacgt ggacatgctg ttgtggatga
      481 tcaccggcgc catcatggtg atgatggtga cgatcaaggt gaccatcgtc cggaacatca
      541 tgcagcgcgc gaaggacccg gcgtttttcc attataacta tttcggcaaa aaaatactgg
      601 atccgggcgt ggtgaagccc ggcgaggtga tggtgttcat gctcaccatg ccgtttttcc
      661 tgctggccgg ggcgtatttc gtcgcccgga tcgtgaactg ggcgttgaag atatgacggg
      721 aggacgcgcc gcgcgatgct aatatccatc tttctggtgt tgatgctgct ctccttcgcc
      781 ctgcacatac tgtttctcgg gcagtatgtg atgacgcggg acaatcgcgc gctcaagcgc
      841 ttcgtcaaca cggccgtgag caatatcacc atcgccgggg ccatgacggt gttggtgctg
      901 ttcaatccct cgctggtgcg ggacatcgac gtcatgttcc tgctctggct cctctccggg
      961 ggcgtgatgc tcatcctgct gggcgtgaag gtcatgatct tccggacgat ctatcggcgc
     1021 agccagatgc ccgagcacta ccatttcaat tttttcggga aaaaggtgct ccacgcgacg
     1081 gtggtcaagc cgatcgaggt gatggtgttc atgatcacca tcccgttttt tcttatatgt
     1141 ggggcctatt ttgtcgcgcg gctcatcaat ctttttctct acaatcgcct gtaaatccca
     1201 atttttactg gattttttcg tgaaagatga tacacgtgca atcatcacgg cgtgcgggtg
     1261 tgtacccgca gccgatggag cacagagaac aggaggatgc catggcgttc ataagcgagc
     1321 atgtaaaaaa gaattccgat aaatgcatga attgtgcgtt tatgaaaatt agagcggtga
     1381 agttggacag acacagtatt ttgtacgacg aggcgaaaat gacattcgag ggccttgtgg
     1441 agggcatgaa ccggggtatg tgcaccatgt gcccaagttg caaggatttc gagaaggtat
     1501 atggggtgaa gccgtacgag tatttcaaat tcagcgatca aaaataaacc tgtgagccgg
     1561 aacgctggtc gccggggaaa ttctgcggga agctgcgagg tggtatatca tatcaaaaac
     1621 ggcggtggtc ctcttgagcg gcggtatcga ttccgcaacg gtcgcgatga tggccgtccg
     1681 tcaggggttt tcggtccatg cgatcacgtt ttcatacgga cagcggcacg ccatcgagat
     1741 cgattgcgca cgccgggtgg ccgcgcagat cggcatcgcc gggcatatcg tgattgacct
     1801 gaaccatgcc gtcttcgcgt cctcgtcgct tgtcgctgaa tcggggatac cggtaccgca
     1861 cgggcgcgat tacgcgcata gcgcggatat cccgagcacc tacgtcccgg cgcgcaatat
     1921 cctgtttctc tcgtacgccc tggcgtatgg ggaaacggtc ggggcccatg atatcttcat
     1981 cggtgtgaac gcgatcgatt acagcggcta tccggattgc cgcccggagt ttatcgaggc
     2041 attcgcccgc atggcggatc tcgggacccg cagcggggcg gaggggcgtg ggttcgtcat
     2101 acacgccccg ctcgtcgaca tgaccaaggc ggagatcatt gcgcacgggc atgcgctggg
     2161 ggtcgactat tccctcacgc acagctgtta tgatcccgac cgggaggggc gctcctgcgg
     2221 gacctgcgat agttgccgga tccgccgcgc gggtttttcc gacgcccatc tggccgatcc
     2281 cacacgctat cggagcgcat catgacggcc ggccgcggtg tgattcgccg ggccgcgtac
     2341 gcgctgctct gcggcgcgat cgacacggtg tttccctcgc ggtgcgcggt gtgcggcgcg
     2401 tcggtgtcgt gggggcggca ttcgctgtgc gcggcgtgcc acgacacgat cccgcgtgcg
     2461 ggcgaccgct gtccgcggtg ctcgggatat ctcgactccg gcgcctgctc cgtgtgcggg
     2521 acgcgggcgt ggtatgtcga tgaaaacatc gtcgtcgcga actaccgggg cgcgatgcgg
     2581 accgcggttc gcaagtataa attttccggg gcgcgccggc tcgcccgtcc gttcgcggag
     2641 cttgccgccc agcggataac tgacgctgag ggcgcgacga cgtgcgatat cgtaaccgcc
     2701 gtgcccatgg ggcgcacgaa gcgctggcgg cgcggataca atcagtccga ggaggtggcg
     2761 cggctcgtcg cgcgccgtct tggggtgccg tatcgccgcc tgctgcggga gaaccgcggc
     2821 gcgctggtgc agaagacgct cggataccgc gagcgctttc tgaatatcct cgggcgtttt
     2881 tcggtttccc cgcgccggat gccggagggc gcgcgggtgc tcgtcgttga cgacattttt
     2941 accaccgggg cgacgatcaa cgagtgcgcc cgggtgctca agggtgcggg cgcgcgcgcg
     3001 gtgtacgggt gcgcgctggc gcgggccgcc gcacacggag acgcggaacg agacgcatga
     3061 gtgcgcagac aaccatttga acgagggtgc catgattaaa gtaatttccg atgacagcgt
     3121 cgagatcgtc cagctcgacg ggcggatcga tcaggaggcc tcggaggagc tcgaggggat
     3181 cctcgagggc cttctgggca agaagaagtt caatatctgc atcgacatga tcaacgtgaa
     3241 gcatatctgc agctcggcgc tgggcgtgat ggtggcgatc aagcgcaaga tcgaaaccaa
     3301 cgacggcgac atcaagctcg tcatcgtcaa cgacaacctc ctcaagcttt ttcaaacaac
     3361 tatgcttgac aaggtcttcg agatttttga aactcagcgg gaatgcctta cggcatttat
     3421 cgactaggcc gcatccggcc tgggacgggg cgccatacat actgcggcat gtacacgaca
     3481 ctaaaatcgg gcctcgacga catcctgcgt cccatcgcgg cgcacctgga gcgggtcgac
     3541 gcggcgatcg gcgagcagct gcgtacgggc gtgccgctca tcgacgagag ctcgatacac
     3601 ctgttcacca acaagggcaa gatgatccgc gcctcgctga tcatcctggc cagcggcatg
     3661 cgcggccgga taccggaggg gatcatcgac atcgcggccg cggccgagat cgtgcatgcg
     3721 gccacgctca tacacgacga tatcatcgac aatgccgaga tgcgccgggg cgacgtcgcg
     3781 gtctcccggc gctggggcaa caaggtggcc gtgctggtgg gcgatcacat gtacacgcgc
     3841 gcgctcgagg tcgccgtgcg cgacgacaat tacgacctct ttcccgagct ggtgcgcgcg
     3901 accgccgata tggtgaaggg cgagttgctg cagatcgaat actcgaacat ggagcgcatc
     3961 accgccgagc ggtatttcga gattatcgag ctcaagaccg cgcgcttcct cggggcgtgc
     4021 gcgaaactgg gcgccatcaa ggccgggttg cccgccgctg aggcggatgc cctcaagcgg
     4081 ttcggcgttc acctggggtt cgccttccag attgtagacg atacgctcga ttgtatcggc
     4141 gacgaacgct cgaccggcaa ggacagcggg aacgattatc tcgacgcaaa ggccacgctg
     4201 ccgctcatca atgcgctgga ggcggcgtcc ggaccggggc gtgacgagct tgtacgcatg
     4261 tgcgcgcagc cggccccgga gcattggccg gccgtgcgcg aggcgatccg cgtgtccggc
     4321 gcccttgcgc gctgtctgga tatcgcgcgg gagtatgcgc ggcgcgcggt gggcgagatc
     4381 gacggtttcc cggacagcga atgcaagcgg atcatacttc gactttccga ttttctgctt
     4441 gagcgcgcgt attgagtatg gcagtgtgga gccggatatt accggcgggg gaggacata
//
LOCUS       DAORHF010000284         4496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20517_length_4496_cov_4.913758, whole genome shotgun sequence.
ACCESSION   DAORHF010000284 DAORHF010000000
VERSION     DAORHF010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20517_length_4496_cov_4.913758"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="PKJ16_16740"
     CDS             complement(<1..1049)
                     /locus_tag="PKJ16_16740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HNV48692.1"
                     /translation="MRICLLCYRGNPYCGGQGIYLKYVAEELVRQGHEVHAIVGPPAP
                     PAMRGVTVHYVPNNEYYVKKGASVINPERPFDIFDPVNLYQYATSRVGAFPEISAFSY
                     RAFAMLRELSQTVRFDVIHDNQCLGYGLLLMQGLGVPVIATIHHPLTVDLKNVIERAS
                     SFANKVKGIFFYPISMQRFVAKRLDHVITVSEDSRDRITRSFGVPAQRQTVVYNGLDR
                     TIFRPLRAVKKVRGKIVFVGNVEDGKKGFLYLVKALSHLDAEVNLTVVDGGSPHRRVS
                     TLLIEKLGLAGRISFTGKTTTDDLVRHYNEAQIAVVPSTHEGFGFPAAEAMACGTPVI
                     ASDGGALPEVVGDAGV"
     gene            complement(1046..1720)
                     /locus_tag="PKJ16_16745"
     CDS             complement(1046..1720)
                     /locus_tag="PKJ16_16745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HNV48693.1"
                     /translation="MANGIPQFDAMDDFLKPTFLGSIVFLTRTYLTYHLSFKKKFAHI
                     GGTPRTWGIQNIDVFGPNIRIGKNCVFAAGRGDRTTITTFRMNGHEGAIDIGDNVLLM
                     SGIRLSSASKIQVGDDCMLANRCYLTDADWHDIYDRTQPVGRTAPIILEKGVWIGDSA
                     IVCKGVRVGENSIVGAGAVVRRDIPSNVIAVGNPAKVVKKLDPKKVVLMSDLYRRIDE
                     WKRRGR"
     gene            1702..1995
                     /locus_tag="PKJ16_16750"
     CDS             1702..1995
                     /locus_tag="PKJ16_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48694.1"
                     /translation="MGSRSPPDHIIEKKYCRIISKLRCTSRAPLTHSMCPGSRYSTSS
                     PAMREMLSLSFAPLPPGKSTRPTLSRKMRSPHASAFFSGQYTQMDPGEWPGVR"
     gene            complement(2394..3239)
                     /gene="lpxC"
                     /locus_tag="PKJ16_16755"
     CDS             complement(2394..3239)
                     /gene="lpxC"
                     /locus_tag="PKJ16_16755"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HNV48695.1"
                     /translation="MSAAMDIPRATVSREIRAEGIGIHSGETVRVRFLPAEPGTGIVF
                     IHAARGRRSPIPARLEHVVETHFAVTLGNGEWKIMTIEHVLAVLSMRGVTDAYVESDA
                     EEMPIFDGSARTLARLFDEASVATLPGSIAPYVVTTPVNVVEGDKFIVALPSPTARLS
                     YIIAYTHPVIQTQYADYPLEPSVFFSEIAPARTYGFEKDVQMLIASGLAKGGSLDNAL
                     VIGETGYINEPRFPDECVRHKALDFIGDLALIGRPVVGHFIACKAGHTLDVAFGRTFL
                     AAYGG"
     gene            complement(3236..>4496)
                     /gene="glyS"
                     /locus_tag="PKJ16_16760"
     CDS             complement(3236..>4496)
                     /gene="glyS"
                     /locus_tag="PKJ16_16760"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HNV48696.1"
                     /translation="PDIVLITEMKEHQKYFAVRDAKGALSPTFLVVSNNPPNDNIRAG
                     NVRVISARFNDARFFFDEDRKTTLADKVESLKTVLFHKELGTIYDKVMRVDRIAARIA
                     DMTGLDAATKALIARAVLLAKADLNSAMVFEFTSLQGQIGKIYAELDGEDPAVARAID
                     DQYRPRFQGDELPSAPVSVVLSLAEKFDNIFGSFSVGNIPGGSSDPYALRRQAGAIID
                     LVVKNGMRLDVKGLLEGVSGQYAGGAGLIDKLLAFITARAKTFFEESGFRYDEIDACL
                     SIGRYDYAELYERARRLNEFRADGQFSQMLMSFKRMNNILASFRRDNPTHALAFSEAN
                     LREDSEKRLHEFFSSRKGEIEGCVARNDCRALFRLLIEGKATVDAFFDGVMVMADDPA
                     VRDNRLALLEGILTPYRNFLDFSKIAE"
BASE COUNT          787 a         1464 c         1426 g          819 t
ORIGIN      
        1 acgccggcgt cgcccaccac ctcggggagc gccccgccgt cgctggcgat caccggcgtg
       61 ccgcaggcca tggcctcggc cgcggggaac ccgaagccct cgtgcgtcga ggggaccacc
      121 gcgatctgcg cctcattgta gtggcgcacc aggtcgtcgg tcgtggtctt tcccgtgaag
      181 gatatccggc cggcgagccc cagcttctcg atgagaagcg tggaaacgcg ccggtgcggc
      241 gagccgccgt ccaccacggt gaggttcacc tcggcgtcca ggtggctgag cgccttgacc
      301 aggtagagga atcccttttt cccgtcctcg acgttcccga cgaacacgat cttcccccgc
      361 accttcttca cggcgcgaag cggccggaag atcgtccggt cgaggccgtt gtacaccacc
      421 gtttgccgct gcgccggaac gccgaacgac cgcgttatgc gatcccgcga gtcctcggac
      481 accgtgatca cgtgatcgag gcgtttcgcg acaaagcgct gcatcgagat cggataaaag
      541 aagatcccct tgaccttgtt cgcgaacgac gaggcgcgct caatgacgtt tttcaaatcg
      601 accgtcaggg ggtgatggat cgtcgcgatc acggggaccc ccagcccctg catcagcagg
      661 aggccgtatc ccaaacactg gttgtcgtgg atcacatcga agcgcaccgt ctgcgacagc
      721 tcgcgcagca tcgcgaaggc gcggtaactg aacgcgctga tctcggggaa ggcgcccacg
      781 cggctggtcg cgtactgata gaggttcacc ggatcgaaga tatcgaaggg tcgctccggg
      841 ttgatgaccg acgccccctt cttcacatag tactcgttgt tggggacgta atggaccgtc
      901 acgccccgca tcgccggcgg cgcgggcgga cccacgatcg cgtgcacctc gtgcccctgg
      961 cgcaccagct cctcggccac gtacttgagg tatatcccct gtccgccgca gtaggggttt
     1021 ccccgatagc agagcaggca gattctcacc ggcccctccg cttccattcg tcgattctgc
     1081 ggtacaggtc gctcatgagg accaccttct tcggatcgag ctttttgacg accttcgccg
     1141 ggttcccgac cgcgatcacg ttggacggaa tgtcccggcg caccacggcc cccgcgccca
     1201 cgatggagtt ttcaccgacg cgcacgccct tgcatacgat cgcggaatcg ccgatccaca
     1261 cgcccttctc gagtatgatg ggggccgtgc gcccaaccgg ctgcgtgcgg tcgtagatgt
     1321 cgtgccagtc ggcgtcggtg agatagcagc ggttggcgag catgcagtcg tcgcccactt
     1381 ggatcttcga ggcgctcgag aggcggatgc cgctcatcaa cagcacgttg tcgccgatat
     1441 cgatggcgcc ctcgtggccg ttcatgcgga acgtggtgat ggtggtccgg tcgccgcgcc
     1501 ccgccgcgaa gacgcagttt ttcccgatgc ggatgttggg accgaagacg tcgatgttct
     1561 ggatgcccca cgtccggggc gtcccgccga tgtgcgcgaa cttcttcttg aacgagagat
     1621 gataggtcag gtaggtgcgc gtcaagaaga cgatgctgcc gaggaaggtc ggcttgagga
     1681 aatcgtccat cgcgtcgaac tgtgggatcc cgttcgccac cggaccacat catcgagaag
     1741 aagtactgcc ggatcatctc gaagttgcgc tgcacgtcgc gcgcgccgct gacgcactcc
     1801 atgtgccccg gcagcaggta ctcgacgtcc agccccgcca tgcgcgagat gctctccttg
     1861 agcttcgcgc cgctcccgcc gggaaaatcg acgcgcccca cgctttcgcg gaagatgagg
     1921 tcgccgcacg caagcgcctt tttctccggc cagtacacgc agatggaccc cggcgagtgg
     1981 cccggggtga gatagacctc caggcccacg ccgttcacct cccaggtccc ctccgtgagg
     2041 accggatcga acgtatattt cgggaagggc atgccgaaca tcgcgaaaaa ctgcgggccc
     2101 gtcgtattca agaattcgat ttcgtccgcg tgcatcgcca ccttcacgcc cttgtccatg
     2161 aaatactcgc tcccctcgag atggtcggga tggcagtgcg tgttcacgac atagttgatg
     2221 tcgtcggggt tgagcccgtc ctttttcatg tccgcgaacc gaaggtccag atggtgcttg
     2281 aggccgggat cgaacaggat gttgagcggc tcgccgaaat agaaggtatt gcagttgttc
     2341 tcgaacacgc cgcgccagac ataggcgtac acgccgtcac acagtttcat gccttagcct
     2401 ccgtatgctg caaggaatgt tcgcccgaag gcgacatcga gcgtgtgccc ggccttgcag
     2461 gcgatgaagt gccccacgac cggccgcccg atgagcgcga gatcgccgat gaaatcgagc
     2521 gccttgtgcc ggacgcactc gtcgggaaag cgcggctcgt tgatatagcc cgtctcgccg
     2581 atcaccaagg cattgtccag gctcccgccc ttcgccaggc cgctggcgat aagcatctgc
     2641 acgtccttct cgaagccgta ggtccgcgcc ggcgcgatct cgctaaaaaa aacgctcggc
     2701 tcgagcgggt aatcggcgta ctgggtctgg atgaccggat gcgtgtaggc gatgatgtac
     2761 gacaggcgcg ccgtcgggga cgggagcgcg acgatgaact tgtcgccctc gacgacgttc
     2821 accggcgtcg tgaccacgta cggcgcgatg gatcccggca gcgttgcgac cgacgcctcg
     2881 tcgaacagac gcgcgagcgt gcgcgccgag ccgtcgaaga tgggcatctc ctcggcgtcc
     2941 gactcgacgt atgcgtcggt gacgccccgc atcgacagga ccgcgaggac gtgctcgatg
     3001 gtcatgatct tccattcgcc gttgcccagg gtcaccgcga agtgcgtctc gaccacgtgt
     3061 tcgagccgcg ccgggatggg cgagcgtcgc ccccgcgcgg catggatgaa gacgatcccc
     3121 gtcccgggct cggccgggag aaaccggacg cgcaccgttt caccggagtg gatgccgatc
     3181 ccttcggcgc ggatctcgcg cgacacggtc gcgcggggga tgtccatcgc cgcgctcatt
     3241 ccgcgatctt cgagaagtcc aggaagttac ggtacggcgt gaggatcccc tcgaggagcg
     3301 cgagtcggtt gtcgcgcacc gcggggtcgt ccgccatcac catcacgccg tcgaagaagg
     3361 cgtccaccgt cgccttgccc tcgatgagca ggcgaaagag cgcgcggcag tcgttgcgcg
     3421 caacgcagcc ctcgatctcc cccttgcggg agctgaaaaa ttcatgcagg cgcttttcgg
     3481 agtcctcgcg gaggttcgcc tcggaaaacg ccagcgcatg cgtcgggttg tcgcggcgaa
     3541 acgacgcgag gatgttgttc atgcgcttga agctcatgag catctgcgag aactgcccgt
     3601 ccgcccggaa ctcgttcagg cggcgcgccc gctcgtagag ctcggcgtag tcgtaccggc
     3661 cgatcgacag gcaggcgtcg atctcgtcgt accggaaccc gctctcctcg aaaaaggtct
     3721 ttgcgcgcgc cgtgatgaag gccaggagct tgtcgataag gcccgccccg cccgcatact
     3781 ggccggacac gccttcgagc aatcccttca cgtccaggcg catgccgttt ttcaccacca
     3841 gatcgatgat cgcgcccgcc tggcgccgca gcgcataggg gtcgctcgag ccgccgggga
     3901 tgttccccac cgagaaggac ccgaagatgt tgtcgaactt ctcggcgagc gagagcacga
     3961 cgctcaccgg agcgctcggc agctcgtcgc cctggaagcg gggacggtac tggtcgtcga
     4021 tggcccgcgc gaccgccggg tcctcgccgt cgagctcggc gtagatcttc ccgatctgcc
     4081 cctggagcga ggtgaactcg aataccatgg cggagttgag gtcggccttg gccaggagca
     4141 cggcgcgggc gatgagcgcc ttcgtcgccg cgtccagccc cgtcatgtcg gcgatgcgcg
     4201 cggcgatgcg gtccacgcgc atcaccttat cgtagatggt gcccagctcc ttgtggaaga
     4261 gcacggtctt cagcgattcg accttgtcgg ccagcgtcgt cttccggtcc tcgtcgaaga
     4321 agaagcgcgc gtcgttgaag cgcgccgata tcacgcgcac gttgccggcc cggatattgt
     4381 cgttcggagg attgttcgag accaccagaa aggtgggcga gagcgcgccc ttcgcatcgc
     4441 gcacggcgaa gtatttctga tgctccttca tctcggtgat aagcacgatg tcggga
//
LOCUS       DAORHF010000285         4495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20524_length_4495_cov_5.116441, whole genome shotgun sequence.
ACCESSION   DAORHF010000285 DAORHF010000000
VERSION     DAORHF010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4495
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20524_length_4495_cov_5.116441"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /locus_tag="PKJ16_16765"
     CDS             <1..970
                     /locus_tag="PKJ16_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV48697.1"
                     /translation="VVARCLAMYGATEKDVIQVAYGYGLFTGGLGLHYGSEKLGALTV
                     PIGGGNTERQLMLIKDFGTTMIACTPSYALNIADYLEKKRPDYDIRKTKLRAGIFGAE
                     PWSEQMRREIESRLGIEAYDIYGLSEVIGPGVSGECSQKSGLHVFEDHFYVEIIDPDT
                     GEVLPEGSKGEIVYTSLTKEACPVIRYRSRDITRLYYDGCACGRTLVKMEKVTGRSDD
                     MLIIRGVNVFPSQIESVLMETEGVEPHYQIIVDRKGALDDIEVKVEVTEKIFSDELKV
                     LEALSRRIADRFRSVLGISAKVTLVEPLAIPRSEGKAVRVIDKRTI"
     gene            987..1418
                     /locus_tag="PKJ16_16770"
     CDS             987..1418
                     /locus_tag="PKJ16_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HNV48698.1"
                     /translation="MNVTQLSIFLENKPGHLQNALKVLSDKKINILTLTIAEAADFGI
                     VRMIVTDAAGAAAALRDQHFTCSTTDVLAIEIEDVPGALLKALESFRKQNLNIEYMYA
                     FTEKRGGRAVMIFRFDDIELAKKALQTEGYNILKKIDIIGA"
     gene            1422..2975
                     /locus_tag="PKJ16_16775"
     CDS             1422..2975
                     /locus_tag="PKJ16_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV48699.1"
                     /translation="MQRKILLGNEAIARGAYEAGCRVATAYPGTPSTEILEAMAAAYR
                     GIKSQWSPNEKVALEVACGAAIAGARSLAAMKHVGLNVAADPLFTFSYTGVNAGLVII
                     NADDPNAWSSQNEQDNRHYARAAKLPMIEPSDPAEAKEYTKYAFDLSEEYDRPVLVRI
                     TTRIAHSQGIVALDDPKDVPLKPFVRNPQKFVMLPSHARPRHIFIEEQLVKLKALSNA
                     SPLNRIEMGKTKRGIITNGVAYQYVKETCPEDSILRIGFIWPLPDDLIRTFADSVDEL
                     YVVEELDPFIEDYVRAMGYAPIGKKIIPLTFELTPEIVDKALNGTTYPTAPLYSGTIP
                     PRPPALCKGCPHGFVFDVLKKLDVTVNGDIGCYTLAALPPYNAMHSQGCMGASISMHL
                     GFEKAQGDEMARNSVAVIGDSTFIHSGITPLIDLVYNKGTGTVLILDNRVTAMTGHQE
                     NPVTGKTLMGEDTYEIKLEELCRAVGVKRVRVVDPKNTAEFEAVVREEVAAPEPSVII
                     SLRKCILKK"
     gene            2986..3561
                     /locus_tag="PKJ16_16780"
     CDS             2986..3561
                     /locus_tag="PKJ16_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009104259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HNV48700.1"
                     /translation="MTNVIFAGVGGQGVILASKILMQVAMNAGYDVKESEVHGMAQRG
                     GSVDCNVRYGEKVYSPLIEKGTCDYLVVFEPLEATRKLEYLKPDGTLIVHTERVNPAP
                     VQAGLAKYPDDIETWIAANITNRMVADFGPVLKAVGSQRALNIVMLGVLSNHLEFTPD
                     QWIEAVKGTVKQNFVEMNVKAFTMGREFTIS"
     gene            3577..4245
                     /locus_tag="PKJ16_16785"
     CDS             3577..4245
                     /locus_tag="PKJ16_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48701.1"
                     /translation="MTRRKAALLTLALAVIFTACSEFRVKTIIDNDKKISKLKKSGIL
                     LRLNDQSLIAHQEFHANLSNWLAGFKPIIDLVILGEQNDAVHHYTRHEERFFQLSTRR
                     NVMETVSKSYLPYKSLGIINLFIRNNAAELKKIMADSSLDSIVIYEVYALVSTEMQNF
                     DFDSVMVIVDRDLNLLYVDHQSDTYDSVETDKAKMKEELLNKVSNRLIARLRRLGFLE
                     RIKL"
BASE COUNT          959 a         1429 c         1361 g          746 t
ORIGIN      
        1 ggtggtcgcg cgctgcctgg ccatgtacgg cgccaccgag aaggacgtga tccaggtggc
       61 ctacggctac ggcctgttca ccggcggctt gggcctgcac tacgggtccg aaaagctggg
      121 cgcgctcacc gttcccatcg gcggcggcaa tactgagcgc cagctcatgc tcatcaagga
      181 cttcggcacc acgatgatcg cgtgcacgcc gtcctacgcg ctcaacatcg ccgattacct
      241 cgagaagaag cgcccggact acgacatccg gaagaccaag ctccgcgcgg gcatcttcgg
      301 cgccgagccc tggtcggagc agatgcgccg ggagatcgag tcgcgcctgg gcatcgaggc
      361 ctacgacatc tacggcctct ccgaggtgat cgggcccggc gtctccggcg agtgctcgca
      421 gaagtccggc ctgcacgtct tcgaggacca cttctacgtc gagatcatcg atcccgacac
      481 cggcgaggtg ctgcccgagg gctcgaaggg cgagatcgtc tacacctcgc tcaccaagga
      541 ggcctgcccg gtgatccgct accgctcgcg cgacatcacc cgcctctact acgacggctg
      601 cgcatgcggc cggacgctgg tgaagatgga gaaggtcacc gggcgctccg acgacatgct
      661 catcatccgc ggcgtgaacg tgttcccgtc gcagatcgag tccgtgctca tggagacgga
      721 gggcgtggag ccgcactacc agatcatcgt ggaccgcaag ggcgcgctgg acgacatcga
      781 ggtgaaggtc gaggtgaccg agaagatctt ctccgacgag ctcaaggtgc tcgaggcgct
      841 ctcgaggcgc atcgccgacc gcttccgcag cgtgctgggc atctccgcca aggtcactct
      901 ggtggagccg ctggccattc cgcgcagcga gggcaaggcc gtccgcgtga tcgacaaacg
      961 aaccatctaa caacggaggc accatcatga acgtaacgca actctcgata ttcctcgaaa
     1021 acaagcccgg gcacctgcag aacgcgctca aggttctctc ggacaagaag atcaacatcc
     1081 tcaccctcac catcgcggag gccgcggact tcggcatcgt gcgcatgatc gtgaccgatg
     1141 cggccggcgc cgcggcggcg ctgcgcgatc agcacttcac ctgctccacc accgacgtgt
     1201 tggccatcga gatcgaggac gtgccgggcg cgctgctcaa ggccctggag agcttccgca
     1261 agcagaacct caacatcgag tacatgtacg ccttcaccga gaagcgcggc ggcagggcgg
     1321 tgatgatttt ccgcttcgac gatattgagc ttgccaaaaa agcgctccag acggaggggt
     1381 acaacatcct caaaaagatc gacatcatcg gagcgtagga catgcaacgg aagattctgc
     1441 tgggcaatga ggccatcgcg cggggcgcct atgaggccgg ttgccgggtc gcgaccgcct
     1501 accccggaac gcccagcacc gagatcctgg aggcgatggc cgccgcctac cgcggcatca
     1561 agtcgcagtg gtcgcccaac gagaaggtcg ccctcgaggt ggcctgcgga gcggcgatcg
     1621 ccggcgcgcg gtcgcttgcg gccatgaagc acgtgggcct gaacgtcgcc gccgatccgc
     1681 tgttcacctt ctcgtacacc ggcgtgaacg cggggctggt catcatcaac gccgacgacc
     1741 cgaacgcctg gagctcgcag aacgagcagg acaaccggca ctatgcgcgc gcagcgaagc
     1801 ttcccatgat cgagccgtcc gatccggccg aggcgaaaga gtatacgaaa tacgccttcg
     1861 acctctccga ggaatacgat cggccggtcc tcgtgcgcat caccacgcgc atcgcgcact
     1921 cgcaggggat cgtggccctg gacgacccga aggacgtccc gctcaaaccc ttcgtccgta
     1981 atccgcagaa gttcgtgatg ttgccctcgc acgcacgccc gcggcacatc ttcatcgaag
     2041 agcagctcgt gaagctcaag gcgctctcga acgcgtcccc gctcaaccgc atcgagatgg
     2101 ggaagacgaa gcgcggcatc atcaccaacg gcgtggccta ccagtacgtg aaggagacct
     2161 gcccggagga ttccatcctg cgcatcgggt tcatctggcc gctgccggac gatctcatcc
     2221 gcacattcgc cgactccgtc gacgagctct acgttgtcga ggagctcgat ccgttcatcg
     2281 aagattacgt gcgcgcgatg ggctatgcgc cgatcggcaa gaagatcatc ccgctcacct
     2341 tcgagctcac ccccgagatc gtcgacaagg cgctcaacgg aacgacgtac cccacggcgc
     2401 cgctgtactc cggcacgatc ccgccgcgcc cgccggcgct gtgcaagggt tgcccgcacg
     2461 gcttcgtctt cgacgtgctc aagaagctgg acgtgacggt gaacggcgac atcggctgct
     2521 acacgctcgc ggcgctgccg ccctacaatg cgatgcacag ccagggttgc atgggcgcga
     2581 gcatcagcat gcacctgggc ttcgagaagg cgcagggcga cgaaatggcg cgaaacagcg
     2641 tcgccgtcat cggcgattcc accttcatcc actcagggat cacgccgctc atcgacctgg
     2701 tgtacaacaa ggggaccggc accgtcctca ttctggacaa tcgggtgacc gcgatgacgg
     2761 gccatcagga gaaccccgtg accgggaaga ccctgatggg cgaggacacc tacgagatca
     2821 agctcgagga gctctgccgc gccgtgggcg tgaagcgcgt gcgagtggtg gacccgaaga
     2881 acacggccga gttcgaagcg gtggtgcgcg aggaggtcgc cgcgcccgag ccgtcggtga
     2941 tcatcagcct gcgcaaatgc atcctgaaaa aatgaggtcg taccaatgac aaacgtaatc
     3001 ttcgccggcg tcggcggcca aggggtgatc ctggccagca agatcctgat gcaggtggcc
     3061 atgaacgccg gctacgatgt aaaggagagc gaggttcacg gcatggccca gcgcggcggc
     3121 agcgtggact gcaacgtgcg ctacggcgaa aaggtctatt cccccctcat cgaaaagggg
     3181 acctgcgact acctggtcgt tttcgagccg ctcgaggcga cccgcaagct cgaatatctg
     3241 aaacccgacg ggacgctcat cgtgcacacc gaacgggtga accccgcgcc cgtacaggcg
     3301 ggactggcga aataccccga cgacatcgaa acgtggatcg ccgcgaacat cacaaaccgc
     3361 atggtggcgg acttcggtcc cgtcctcaag gccgtgggct cgcagcgggc gctcaacatc
     3421 gtgatgctgg gcgttctctc gaaccatctc gagttcacgc cggaccagtg gatcgaggcg
     3481 gtcaaaggga ccgtgaagca aaattttgtt gaaatgaacg taaaagcctt taccatgggc
     3541 cgtgagttta caatctcata aggcagggat gatgcgatga cccgacgcaa agcggcgctt
     3601 ctcacgctgg cgctcgccgt gatcttcacg gcatgctccg agttccgcgt gaagaccata
     3661 atcgacaacg ataaaaaaat atcgaagctc aaaaaatcgg ggatccttct gcgcctgaac
     3721 gaccagagcc tcatagcgca ccaggagttc cacgccaacc tctccaactg gctcgcgggc
     3781 ttcaagccga tcatcgacct ggtgatcctg ggcgagcaga acgatgcggt ccatcattac
     3841 acccggcacg aggagcgctt cttccagctc tcgacgcggc gcaacgtgat ggagacggtc
     3901 agcaagagct acctgcccta caaatcactg ggcatcatca acctcttcat tcgcaacaac
     3961 gccgccgagc tgaaaaaaat catggccgac agctccctcg acagcatcgt catctacgag
     4021 gtgtacgcgc tcgtctcgac cgagatgcaa aacttcgatt tcgattcggt gatggtgatc
     4081 gtcgaccgcg atctcaacct gctctacgtt gaccatcaga gcgacaccta cgacagcgtc
     4141 gaaacggaca aggccaagat gaaggaagag ctgctgaaca aggtttcgaa caggctcatt
     4201 gcgcgcctcc gcaggctcgg ctttcttgag cgtataaagc tgtagcggca catatttctt
     4261 acgaacgaac tcattcgatc gaaactcagt atcgggatgg gccgatacga tcaaacacaa
     4321 gcgaggttgc acaatgaata agctggtatc ttccaccgtg gtcgttctgg gcctcttgct
     4381 cgccgttgtg ctggtgagcg gcatatcgtc gatgggcatc gccgccccga aggcgggggg
     4441 ctcctgcacc ggcatcgggc cgatggaggg tcgtgtggcc tgcgacggca agaag
//
LOCUS       DAORHF010000286         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20532_length_4494_cov_4.617031, whole genome shotgun sequence.
ACCESSION   DAORHF010000286 DAORHF010000000
VERSION     DAORHF010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20532_length_4494_cov_4.617031"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2829)
                     /locus_tag="PKJ16_16790"
     CDS             complement(<1..2829)
                     /locus_tag="PKJ16_16790"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV48702.1"
                     /translation="MSLFVRAALACAVVCALCGTAHSFGKNKVNREVFDWEMLRTIHF
                     DVYYPRGMDALGAHAGRLCEEGYVHIANYLRHELTDPVPVIIYPSHIAFQENNIIPNI
                     LGEGTGGFTESFKTRVVVPFNGSYADLRHVLTHELVHAFQYNILFNDTTGALLSRFGR
                     RGLPLWIMEGMAEYLSIGYDETADMVMRDIIFNDKYATLADLTKYNIHSGYLLYKQGQ
                     SFYYYLEETYGRRAIGEFFRDVRDVGDLDTVFLALTGKTMEEVSLDYIRYFKRRYYPI
                     IDGKKFEEEEGEQITHHLKTRSSFNSCPAVSPDGARIAFLSNTDVYSNVVIVDITKKK
                     KERTITTLVEGETSSRFEGMHLLSNNLSWSRDGKRLAFVAQSHGRDAVFIVDPANGRV
                     LDRIELDFRAVLDPALSPDGGSIAFIGQGDGAADLYLYRIAEKKLSRITNDRFSERHP
                     RISAKGDFLVYSSNWNGAGDVERGAYNIYRHDIATGKRTTLVASPGNDLQADLSHDDS
                     RIVFISNRKGIYNAYVRDLSSGEESRLTNVLCGVFYPRWFPDGTRIAYVGYQNLGYDI
                     FTREAKGAGGGTDDSRETPLKRVGFPAAYFDMKNAAQMPYAARITNDFIAFGVAATTG
                     YGLAGIAQAGFSDFMGDHRVIFTANYFQYNENGVLNFDAVYFYLKHRWDFGAGVFRKE
                     NPFGVFSLETINELIHNVYWDTERMYHYGAYGIARYPFTRFARLNLRATTSRYEVDYT
                     EYSPRRDVAGNLHQVSASLSYDNVIWGYLGPLDGIRGQAEVEEAIPAGADSFDYGTVS
                     VDIRKYFLIRKRYVLALRGSAGHVFGRDSQQFKYYLGGFTNLRGYPFFEFSGENVFFT
                     RAEFRFILVEHLKFGWPLFFRMVPIGGVLFADAGSVWNRNVYRFNDPETGKFDDFKVD
                     FGFGFRITLFPIIFKLDYAWPYNNKSFDS"
     gene            3022..3203
                     /gene="ssrS"
                     /locus_tag="PKJ16_16795"
     ncRNA           3022..3203
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PKJ16_16795"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3449..3973)
                     /locus_tag="PKJ16_16800"
     CDS             complement(3449..3973)
                     /locus_tag="PKJ16_16800"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HNV48703.1"
                     /translation="MKANERLNALEVALNNEMNERQFYLNNAERTRNPVGKAMFARIA
                     DDELEHYEMLKKLHAEWTANGKWPDTVPLTVNKTSVKTILQRMVDESKDAPASDSDDL
                     AAVQAAAQFESKGVDLYRELAEASTDKKEKEFFMLLSGIEREHFLSLKDMEEFIRNPS
                     AWYTAKERHGMDGA"
     gene            complement(4080..>4494)
                     /locus_tag="PKJ16_16805"
     CDS             complement(4080..>4494)
                     /locus_tag="PKJ16_16805"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HNV48704.1"
                     /translation="KELAARDGIAEPRFFTDGPDVHLKNCIACSLCTQVCEDVVGVSA
                     ISMVNRGREKQPAAPYMKASNACIGCGACAYACPTGAIEMTDTPDARTIWGKTFEMQK
                     CSVCSTPFIPKEQAEWIIKTTGKDRAFFEKCPTHR"
BASE COUNT          950 a         1332 c         1228 g          984 t
ORIGIN      
        1 ggaatcaaac gatttattat tatacggcca ggcgtagtca agcttaaaaa tgatgggaaa
       61 cagcgtgatg cggaaaccga agccgaaatc gaccttgaaa tcgtcgaact tcccggtttc
      121 cgggtcgttg aagcgataca cgttcctatt ccagaccgaa ccggcatcgg cgaagagcac
      181 gccgccgatg gggaccatcc gaaagaagag cggccagccg aacttgaggt gctcgacaag
      241 gataaaccgg aactccgccc gcgtgaaaaa gacgttttcc ccgctgaact cgaaaaacgg
      301 atacccgcgt aaattggtaa acccgccgag atagtacttg aactgctggc tgtcgcgacc
      361 aaagacgtgg cccgcgctgc cgcgcagcgc gaggacgtac cgctttcgga tgaggaagta
      421 cttccgtata tcgacgctca cggtgccgta atcgaaggag tccgcgcctg cggggatcgc
      481 ctcctcgact tccgcctgac cgcgaatccc gtccaatgga ccgaggtacc cccagatcac
      541 attgtcgtag ctcagggacg ccgacacctg gtgcaaattc cccgccacgt ccctgcgggg
      601 gctgtactcc gtatagtcca cttcatatcg gctcgtcgtg gcgcgcagat tcaaccgcgc
      661 aaagcgcgtg aaggggtatc gggcgatccc ataggccccg tagtgataca tgcgctccgt
      721 gtcccaatag acgttgtgga tcagctcgtt gatggtctcc agcgagaaga cgccgaaggg
      781 gttttccttg cgaaacacgc ccgcgccgaa atcccagcgg tgcttcaggt aaaaatacac
      841 cgcatcgaaa ttgagcacgc cattttcgtt gtactggaaa tagttcgcgg tgaatatcac
      901 gcgatgatcg cccatgaagt cggaaaaccc cgcctgggcg atccccgcca ggccgtagcc
      961 ggtggtcgcc gccacgccaa atgcgataaa atcgttggtg atacgagccg cgtagggcat
     1021 ctgtgcagcg tttttcatgt caaaataggc ggcgggaaac ccgacccgct tcagcggcgt
     1081 ctcgcgcgaa tcatccgtac cgcctcccgc gcccttggcc tcgcgcgtga aaatgtcgta
     1141 ccccaggttc tggtagccca cataggcgat tcgcgtccca tcggggaacc agcgcggata
     1201 gaacacgccg cagagcacgt tcgtgaggcg cgactcctcc ccgctcgaca gatcgcggac
     1261 ataggcgttg tatatccctt tccgattgga gatgaaaacg atccgggagt cgtcgtgcga
     1321 gaggtccgcc tgcaaatcgt tgccgggaga ggccaccagc gtcgtgcgct tcccggtcgc
     1381 gatgtcgtga cgatagatat tgtacgcgcc gcgttcgaca tcgcccgccc cgttccagtt
     1441 cgaggaatag acgagaaaat cgccctttgc ggagatgcgc ggatgtcgct ccgaaaaacg
     1501 gtcgttcgtg atccgcgaaa gttttttctc cgcgatccgg tagagataca ggtccgcggc
     1561 gccatcaccc tgcccgatga atgcgatcga gcccccgtcc ggcgagagcg cggggtccag
     1621 caccgcgcgg aaatcgagct cgatgcggtc cagcacccga ccgttcgccg gatcgacgat
     1681 gaacaccgcg tcgcgcccgt gcgattgcgc cacgaaggca agccgcttcc cgtcgcggct
     1741 ccagctcagg ttgttgctca acaggtgcat gccctcgaac ctcgagctgg tctcgccttc
     1801 gaccagggtg gtgatcgttc gctccttctt tttcttcgtg atatcgacaa tcaccacgtt
     1861 cgagtacacg tccgtgttcg agagaaacgc gatacgcgca ccgtcgggcg aaaccgccgg
     1921 gcagctgttg aacgaggagc gcgtcttcag gtggtgcgtg atctgttcgc cctcctcctc
     1981 ctcgaatttc ttgccgtcga tgatgggata gtaccgacgc ttgaaatagc ggatatagtc
     2041 gaggctcacc tcctccatgg ttttgcccgt gagcgcgaga aaaacggtgt cgaggtcccc
     2101 cacgtcgcgc acgtcgcgaa agaactcgcc gatggcccgg cggccgtagg tctcctccag
     2161 gtaataataa aaggactgcc cctgcttata aagcaagtac ccgctgtgga tattgtattt
     2221 cgtgaggtcc gcgagggtcg cgtatttgtc attgaagatg atgtcgcgca tcaccatgtc
     2281 ggccgtttca tcgtacccga tcgagaggta ctcggccatc ccctccatga tccacagggg
     2341 cagtccccgt cgtccgaatc gcgacaggag cgcgcccgtg gtgtcattga aaaggatatt
     2401 gtactgaaag gcgtgcacga gttcgtgcgt aagcacatgg cgaaggtccg catacgatcc
     2461 gttgaacggc accaccacgc gcgtcttgaa cgattcggta aagccgcccg tcccctcgcc
     2521 gaggatgttc gggattatgt tgttttcctg aaatgcgata tgcgacggat agatgatcac
     2581 cggcaccggg tcagtgagtt cgtgccgaag atagttggcg atgtggacat agccctcttc
     2641 gcagagccgg ccggcgtgcg cgccgagtgc gtccatgccg cggggatagt acacgtcgaa
     2701 atggatcgtc cgcagcatct cccagtcgaa aacctcgcgg ttcaccttgt tcttcccgaa
     2761 cgaatgcgcc gtgccgcaga gcgcgcagac gacagcgcag gcgagcgccg cccgtacgaa
     2821 tagcgacact gcacgtcgac atgtccttcc ggccgttatc atatcgtttt tttggcgcgc
     2881 cgaaaccgaa cgagcagaga cggctccctc ttcttaaaat cggtccgcgt gaatgaaaag
     2941 ctgagggctt tcccgctcgt ttattttttt accgaaaggt gtttttttat tgaattatac
     3001 agcacgggtt ttgtacggtc gtaccacctg gggtgctcgc gattagattt aggtaaattt
     3061 tgaaccaata cttaccacga agggaaccca atatttgatg gccaaaatca tgcttcctcg
     3121 agaagaaggt tgaacccatc gtgagggcgc ccacctgtta atcaggttca aaattcgttt
     3181 ctacacggca ccccgggttt attatccggc agaggtcatt cgaggtattg ggatttccaa
     3241 agggccgggc tgactcggtt gcactatctc aacccacgaa gacttccgac agcgccattc
     3301 tgcccatgcg cgcgaaaaca caacgcagcc ctcgggcacg ccgcgcctgc tgctccatgg
     3361 aaatactatt aatcgctcaa ctatgattgg aacaccgggg gagccgctcg cctcgccccg
     3421 gagtcacttc tggaagggag tatgacgttt acgcgccgtc cataccgtgg cgttctttcg
     3481 ccgtgtacca tgcggacgga tttcgaataa actcctccat gtccttcaac gaaaggaaat
     3541 gctcgcgctc aatcccggaa aggagcatga aaaactcttt ttcctttttg tctgttgagg
     3601 cctcggcgag ttcgcgatac agatcaacgc cctttgattc aaattgcgca gcggcttgga
     3661 ccgcggcaag atcgtcgctg tccgacgcag gcgcatcttt cgattcgtcg accatcctct
     3721 gtaaaatcgt tttaacgctt gtcttgttaa cggtcagcgg aacggtgtcc ggccatttcc
     3781 cgttcgccgt ccattccgca tgcagctttt tcagcatctc ataatgctcc agctcgtcat
     3841 ccgcaatgcg ggcgaacatc gcttttccaa cgggattacg cgtccgttcc gcattattaa
     3901 gataaaactg gcgttcgttc atttcattgt taagcgccac ttcaagagcg tttagccgtt
     3961 cgttcgcttt cattgttcac cttcgtcatg ctggaaaata accacccaca cagtggagtg
     4021 tttaaaatca attccacgcc aggtagtggc tttcaatcac acccgccatc gtccgcaccc
     4081 tatcggtgcg tcgggcactt ctcgaaaaac gcacggtctt tccccgtcgt tttgatgatc
     4141 cattcggcct gctccttcgg gataaacggc gtgctgcaga cgctgcattt ctgcatttca
     4201 aacgtcttcc cccagatggt tctggcgtca ggcgtgtcgg tcatttcaat cgcccccgtg
     4261 ggacatgcat aggcgcacgc cccgcacccg atgcaggcgt tgctcgcctt catatacgga
     4321 gcggcaggct gtttttccct cccgcggttc accatggaga tggccgagac gccgaccaca
     4381 tcctcgcaga cctgcgtaca gaggctgcag gctatgcaat ttttcaggtg aacgtccgga
     4441 ccgtcggtga aaaacctcgg ctcggcgata ccatcccggg cggcgagttc cttg
//
LOCUS       DAORHF010000287         4450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20846_length_4450_cov_3.936974, whole genome shotgun sequence.
ACCESSION   DAORHF010000287 DAORHF010000000
VERSION     DAORHF010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20846_length_4450_cov_3.936974"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="PKJ16_16810"
     CDS             complement(<1..829)
                     /locus_tag="PKJ16_16810"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HNV48705.1"
                     /translation="MLKQPITRIRDKNRIINNIINVMSEHKTFLVLGHTSPDEDCVSS
                     MVAIALLLNRFDKAVTINIAKKTHDNVSYLLRICKFNAIKIVHNPSKLPRTPDTVIIC
                     DTAKRSMLERSKAIDKLLADPKIIKIEIDHHIAGDSEHLGDPDYALVTAASSASELVG
                     LIGLKLKGRRDVLSRFMVLDPLSRNFVLAVITGIIGDTQKGRYLKSRREKRYYDIFTC
                     MFEDILMKSTVRETNFFKIDDVFYLKTIEDGIRLGFTESDCSLIVETNKKMIGALKAV
                     "
     gene            1027..3315
                     /locus_tag="PKJ16_16815"
     CDS             1027..3315
                     /locus_tag="PKJ16_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48706.1"
                     /translation="MVLKKRIIFIVIVVLVALLSLYVIGFNAFTYVFFPDRMVKDTLV
                     GFIRQNFNKAIRFDDAFVDISGNIIVTGLNVSHTSDFNDNVSLVKCERAVIDTSFVSL
                     LSRSIVVHGVTFRGAEVTLMKRAGVSYADNFKRLFVLSRPLAEIPEINRRSFLVDIFD
                     SRLVYRDMQEDDALVLQSEATDIAVRIDGENVRYRVSGKLQPHRGADVGTGRLRVSGT
                     VILPNDGKSPHSEASVEVAGLDITLLNPLFAGRLRSDLFIRGLLGVSAKIATREGRSV
                     ADASVEVEDLVAERVSSESGAVALVSDLSFKARGRIEASNDANEITLRDVRINDGNLD
                     CRADVRYRNLPKDRTVDLALRTNRFDLARLTRSLAVLRGTDVAGFARLDARLIFDHAQ
                     RQPRDVRASLDADGFSLARNRPEGALSLMRDGALRLRLDGDRLAGNASVASGDSRSSV
                     VVQSVVKSWTPFASQTMLSVFSDRLTSRALVPLATAAFDALHALAMDDRRYAYDEIPF
                     PEKPLGKLVNGNTVMITCAATTLVLSPANPLRGLFFSLILSDGSIKLKHFNLEGYGGG
                     YSADVFCNFTSGYPFMSFKAKAKNMDLGAFAKDAGWRGTIGGLMNAEIEYQASVSKPS
                     QFIENGQGALSIEVYPAELSGTAIQSALIAHCRDNGIDATALQSLKVARAWVTLRQSR
                     EQFFVSRLNVTSDVLNLDASGTFPTGPELSIPFTAKLTTNADDKTAGAVSLPAVVKGV
                     LSRPLLFLGTGDRRGMPLFHLK"
     gene            complement(3298..>4450)
                     /locus_tag="PKJ16_16820"
     CDS             complement(3298..>4450)
                     /locus_tag="PKJ16_16820"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV48707.1"
                     /translation="TGRMDINFIGAHGSFKNYPYYYFHRGGTMDNKSLEKKIVLVAAY
                     AATGVASDMHGSPYGTLYGIEHHASAINTILNQDFIVKLAAWQNLLIMLAIALLLGLL
                     LTRLSIIKSTVLTVVLIAGYAVAGYLFFEFRNVIVAYATPIIQMATTFTFIIVYRVLT
                     EQKEKKFIKQTFSKFVSHSVVEELLQDPERLKLGGDKKNLTVLFSDIRGFTTISERLT
                     PEALVVHLNEYLQEMTDVVFKYDGTLDKYVGDEIMAFWGAPIPQEDHALRACRTALEM
                     MTRLRELNENWKSQGKPPLDIGIGINTGEMVVGFMGSSSRMDYTLMGDMVNLGARLEG
                     TNKAYGTNIIISEFTYEHVRDHVIVRELDLVRVKGKLKPVKIYELFEMK"
BASE COUNT          778 a         1352 c         1396 g          924 t
ORIGIN      
        1 agacggcttt gagggcgccg atcatcttct tgttcgtctc aacgatgagg gaacagtccg
       61 actccgtgaa gcccaggcgg atcccgtctt ctatggtctt caggtagaag acgtcgtcga
      121 tcttgaagaa gttggtctcg cgcaccgtcg atttcattag gatgtcctcg aacatgcacg
      181 tgaagatgtc gtagtagcgc ttttcccggc gcgacttgag gtagcgtccc ttctgcgtgt
      241 cgccgatgat gccggtgatg accgcgagca cgaagttgcg cgacagcgga tcgagcacca
      301 tgaaccgcga gagcacgtcc cgccgcccct tgagcttcag gccgatgagc cccaccagtt
      361 ccgaggccga cgaggccgcg gtgaccagcg cgtagtcggg gtcgcccagg tgttcgctgt
      421 ccccggcgat atggtggtcg atctcgatct tgatgatctt cgggtcggcc agcagcttgt
      481 cgatcgcctt cgagcgctcg agcatggagc gcttcgcggt gtcgcagatg atcacggtgt
      541 cgggcgtgcg ggggagcttc gacggattgt gcacgatctt gatcgcattg aacttgcaga
      601 tgcgaagcag gtagctcacg ttgtcgtggg tctttttcgc gatgttgatg gtgaccgcct
      661 tgtcgaatcg gttgaggagc agcgcgatgg cgaccatcga cgagacgcaa tcctcgtccg
      721 ggctggtgtg ccccaggacc aggaaggtct tgtgttcgct catgacgttg atgatgttgt
      781 tgatgatccg gttcttgtcc cggatgcggg ttatcggttg cttgagcatt atgtcgccta
      841 tgggtccgct gcaaatgagc atggcgctct tttatgaaaa agtcaataga aaatattttt
      901 tcccgcagtc aaaaaaacct ccggacgcga tcaagacgct aaaaaaggct tgcggcgggc
      961 gcacggtgcg gcgaatacta ccagagcgac ccgtcgggcg caaaaacagt cgcggaatct
     1021 gcagacatgg tcctcaaaaa acggattatc ttcatcgtca tcgtggtcct ggtcgccctg
     1081 ctgtcgctgt atgtgatcgg gttcaacgcg ttcacctatg tctttttccc cgaccgcatg
     1141 gtcaaggaca cgctggtcgg cttcattcgg cagaatttca acaaggccat ccgcttcgac
     1201 gacgccttcg tcgacatatc cgggaacatc atcgtcaccg ggctcaatgt gtcgcacacc
     1261 agcgatttca acgacaacgt gagcctcgtc aagtgcgagc gcgccgtgat cgacacctcg
     1321 tttgtgtcgc tgctttcccg gtcgatcgtg gtgcacggcg tcacgttccg gggcgcggag
     1381 gtcacgctca tgaaacgcgc cggggtctcc tacgcggaca atttcaagcg cctcttcgtc
     1441 ctgtcgcgtc ccctcgctga gatacccgaa atcaaccgcc ggagcttcct ggtcgatatt
     1501 ttcgattccc ggctggtcta tcgcgacatg caggaggacg atgcgctcgt cctgcagtcc
     1561 gaagcgacgg acatcgctgt tcgtatcgat ggcgagaatg tccggtatcg ggtatccgga
     1621 aaactgcagc cgcatcgggg cgcggacgtc ggcaccggcc gcttgcgggt ctccgggacg
     1681 gtcatacttc cgaatgacgg gaagtcgccg cactcggagg cgtcggtcga ggtcgcgggg
     1741 ctggatatca ccctgctcaa tcccctgttc gcgggccgcc tgcgaagcga cctgttcatt
     1801 cgcgggctcc ttggcgtttc ggcgaagatc gcgacccgcg agggtcgttc ggtcgcggac
     1861 gcctcggtcg aggtcgagga cctcgtcgcg gagcgcgtct cgagcgagag cggcgccgtt
     1921 gccctggttt cggatctctc cttcaaggcg cggggacgca tcgaggcttc gaacgacgcg
     1981 aacgagatta ccctgcggga cgtgcggatc aacgacggga atctcgattg ccgcgccgat
     2041 gtccgctatc gaaacctccc gaaggaccgc accgtcgatc tggccctgcg gaccaaccgc
     2101 ttcgatctcg cgcgcttgac ccggtccctg gccgtgctcc gcgggaccga cgtggccgga
     2161 ttcgcccggc tggacgcgcg tctcatcttc gaccatgcac agcggcagcc ccgcgacgtg
     2221 cgcgcctcgc tggatgccga tgggttctcc ctcgcgcgca accgcccgga gggggcgctg
     2281 tccctgatgc gcgacggcgc gctcaggctc cggctcgacg gcgaccggct ggcgggcaac
     2341 gcgagcgtcg caagcgggga ttcacgctcc tccgtggtcg tgcagtcggt cgtaaagagc
     2401 tggaccccgt tcgcttccca gacgatgctg tcggtctttt ccgatcggct cacctcgcgc
     2461 gccctcgtgc ccctcgcgac ggcggccttc gacgcgctcc atgcgctcgc catggacgac
     2521 cggcgctatg cttacgacga gataccgttt cccgagaagc cgctggggaa gctggtgaac
     2581 ggcaataccg tgatgatcac ctgcgcggcc accacgctgg tgctgtcgcc ggcgaacccc
     2641 cttcggggcc tgttcttctc gctcatcctg agcgatggct ccatcaagct caagcacttc
     2701 aatctcgagg gctacggggg gggctattcg gcggacgtgt tctgcaactt caccagcggc
     2761 tatcccttca tgagcttcaa ggcgaaggcg aaaaacatgg acctgggggc gttcgccaag
     2821 gacgcggggt ggcgggggac catcggcggc ctcatgaacg ccgagatcga ataccaagcc
     2881 agcgtgagca agccctcgca gttcatcgag aacggccagg gagccttatc gatcgaggtc
     2941 tatcccgcgg agctcagcgg cacggcgata cagtcggcgc tgatcgcgca ctgccgtgac
     3001 aacggcatcg acgcgacggc gcttcagtcg ctcaaggtcg ctcgggcgtg ggtcaccctg
     3061 cgacagtcgc gcgaacagtt cttcgtgagc cgcctgaacg tcacctcgga cgtcctgaac
     3121 ctcgacgcga gcgggacctt ccccacgggc ccggagcttt ccattccgtt taccgcaaag
     3181 cttacgacga atgcggacga taaaacggcc ggcgctgttt ccctgccggc cgtcgtcaag
     3241 ggcgtcttgt cccggccgct cctgtttctg ggaacgggcg accggcgcgg catgccgcta
     3301 tttcatctca aatagctcgt agatcttcac gggcttcagt tttcccttca cccgcaccag
     3361 gtcgagctcg cgcacgatca cgtggtcgcg gacatgctcg taggtgaatt cgctgatgat
     3421 gatgttggtg ccataggcct tgttggttcc ctcgagccgc gcgcccaggt tcaccatgtc
     3481 gcccatgagc gtgtagtcca tccgcgagga cgatcccatg aagcccacga ccatctcgcc
     3541 ggtgttgatg ccgatcccga tgtcgagcgg cggcttgccc tgcgatttcc agttttcgtt
     3601 gagctcgcgg agcctggtca tcatctccag cgccgtgcgg caggcgcgca gggcgtggtc
     3661 ctcctgcggg atgggcgcgc cccagaaggc catgatctcg tcgcccacgt acttgtccag
     3721 cgtgccgtcg tatttaaaga cgacgtccgt catctcctgc agatactcgt tcaggtgcac
     3781 cacgagcgcc tcaggcgtga gccgctcgga gatggtggtg aatcctcgga tgtccgagaa
     3841 gagcaccgtg aggttctttt tgtcgccgcc cagcttgagg cgttccggat cctgcagcag
     3901 ctcctcgacc accgagtgcg acacgaactt ggaaaaggtc tgtttgatga acttcttctc
     3961 cttctgctcg gtgagcacgc ggtagacgat gatgaaggtg aacgtcgtgg ccatctgtat
     4021 gatgggcgtg gcgtaggcga cgatgacatt cctgaactcg aagaagagat accccgcgac
     4081 ggcgtagccc gcgatcagca cgaccgtgag gacggtggac ttgataatgg acaagcgcgt
     4141 gaggagcaag cccagcagca gggcgatcgc gagcatgatg agcaggttct gccaggcggc
     4201 cagtttcacg atgaagtcct ggttgaggat cgtgttaatg gcgctggcgt ggtgctcgat
     4261 gccgtagagc gtcccgtagg gcgatccgtg catgtcgctc gcgacccccg tcgcggcgta
     4321 ggcggccacc aggacgatct tcttctcgag cgacttattg tccatggtgc cgccgcgatg
     4381 gaagtagtag taggggtagt tcttgaagct tccgtgcgcg ccgatgaagt tgatgtccat
     4441 gcgccccgtt
//
LOCUS       DAORHF010000288         4449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20852_length_4449_cov_3.675011, whole genome shotgun sequence.
ACCESSION   DAORHF010000288 DAORHF010000000
VERSION     DAORHF010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20852_length_4449_cov_3.675011"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PKJ16_16825"
     CDS             <1..199
                     /locus_tag="PKJ16_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48708.1"
                     /translation="QGWLRHYVVDDDPRSRYQCDECRCVWDSTFRMLTPIGRPHNAAS
                     TLGTNPVLVRDYEHPDDDHRP"
     gene            complement(204..1520)
                     /gene="rodA"
                     /locus_tag="PKJ16_16830"
     CDS             complement(204..1520)
                     /gene="rodA"
                     /locus_tag="PKJ16_16830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HNV48709.1"
                     /translation="MLKRNEIYKIDYLLVVAMALIVLIGILMIYSAGFDPIEKVNSGL
                     FKKQLLWFIIGFVLMIALTFVNYNWLGDYSLHIFIVILLMLLFTVIFGRPVRNSKAWI
                     TLGVLSIQPSEFMKLSLIIILAKYLELREMEIGHFRELVIPALLTFVPIIIILLQPDF
                     GTALVFIPILFTMLFVGGADITHLVSIILIATIATVVPMVITYREWVGEESANMLLDL
                     FKNTDLIFVISGVLILIALAAFILHFFFVKKFYRRIYVPASVMSMGLFFSVIIMKYLK
                     EYQKKRLLVFLNPDLDPHGSGYNVIQSKIAVGSGGFLGKGFLKGSQAQLGFLPEKSSD
                     FIFSVVAEEWGFIGSIVLVGLLGVIIYKGIYIALESKDKFGALLASGITTFFFFHMLI
                     NIGMGIGIMPVTGLPLTFVSYGGSNLIVSMMCIGILINIRMRKFVY"
     gene            complement(1504..3321)
                     /gene="mrdA"
                     /locus_tag="PKJ16_16835"
     CDS             complement(1504..3321)
                     /gene="mrdA"
                     /locus_tag="PKJ16_16835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HNV48710.1"
                     /translation="MLEAFRMRMFFFLGVVTLVIFVIVVQIINLQLIRGDEYREKSRM
                     NMENYVPIPAARGEIYDRNFNPNAKSVVIVSNRPSFNVTMVPSNFKKKDEYRTVMRRL
                     CKLLALPYDETVEGIAKLNPWERVTIKEDVDFNSIVTLATHQDKFPFIDWEASTVRVY
                     NFGNMFAHAVGYTGSISREEFKKLRNFGYRHYQKVGKAGLESEHDILLRGSDGYIRRI
                     VDVRKRTDVEEVGLHPVSGSNMVLTIDYEIQRIAHEAMEAHPKGSVIVMKAASGEVLS
                     LLSKPDYDPNLIISKDNVTTIKDLMEDKERPFLNRAIMSKYPPASTFKLMTAIAALES
                     ERWDPATQLYCPGKYTLKGYVDKDFFCYDVHGTNNMEWAICKSCSVYFYQVGLRTTPT
                     RILEYAGYFGLGEKTGINLPGEIPGFIPSGKWKQKTFGQTWYDGDTVNLSIGQGFISA
                     TPIGMAAFVSGIVNNGIIFKPNLVREVRSQDNRRVVQTFRPVKMREIPLSQRTLAVVK
                     NGMRMVVASGTASRLSGLKVPVAGKTGTAQTRSKRMEDASQHAWFVGYAPFDAPPEQA
                     IVVVVMVEYGVAGAATAVPVAERIFGKLLSQGYFNAEKK"
     gene            complement(3352..3852)
                     /gene="mreD"
                     /locus_tag="PKJ16_16840"
     CDS             complement(3352..3852)
                     /gene="mreD"
                     /locus_tag="PKJ16_16840"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HNV48711.1"
                     /translation="MIFTYLITAGLIIASLVIQGHPSFDVLRIAGVKPDLLFIIVIYF
                     GYSFGSFYGEVTGFVGGLLHDAISNSPIGFLAFSKMALGFVVGMFGRAVFKENILTVL
                     LLLFIASLAKGVLSLFLAFVFHQAAVSDILSIIIPEAFYNAILAPPLFFLLDRLFEHE
                     LSREGY"
     gene            complement(3854..>4449)
                     /gene="mreC"
                     /locus_tag="PKJ16_16845"
     CDS             complement(3854..>4449)
                     /gene="mreC"
                     /locus_tag="PKJ16_16845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HNV48712.1"
                     /translation="EELSEIRRENDRLRAMLSMRPQVQFESIPARIISKDPDNWFRTI
                     VIDRGTGDGIAVNMPVIGFNGENKAVVGKVIEVRRFVSRIQPIISGTMKLGVVFQESR
                     YPGLLSGFTANSSLCLIDYISKSTVVKFGDLVVTSGQGGIFPAGLMVGKVAKTNLMDS
                     NSFQRVLVQPIIDFDRIEEVYVIKKEPDKAMLELLGE"
BASE COUNT          958 a         1293 c         1360 g          838 t
ORIGIN      
        1 ccagggatgg ctgcggcact acgtggtgga cgacgatccg cgcagccgct accagtgcga
       61 cgagtgccgc tgcgtgtggg attccacctt tcggatgctc acgccgatcg gtcgccccca
      121 caatgcggcg agcaccctcg ggacgaaccc ggtgctcgtt cgcgactatg aacaccccga
      181 cgacgatcac cggccatagc cggtcaatac acgaacttgc gcatgcggat gttgatgagg
      241 atgccgatgc acatcatcga cacgatgagg ttcgatccgc cataggagac gaaggtgagc
      301 gggaggccgg tcaccggcat gatgccgatc cccatgccga tgttgatgag catgtgaaaa
      361 aagaagaagg tggtgatgcc gctggccaga agcgcgccga atttgtcctt cgactcgagc
      421 gcgatgtaga tccccttgta gatgatcacg cctaaaagac cgaccagcac gatcgacccg
      481 ataaagcccc attcctcggc caccacggag aagatgaagt cggacgattt ctccggaaga
      541 aacccgagct gcgcctggct gcccttaagg aagcccttgc ccaggaagcc gccggagccc
      601 acggcgattt tcgactggat cacgttgtac cccgatccgt gcgggtcgag gtccgggttg
      661 aggaacacca ggaggcgctt cttctggtac tccttgaggt atttcatgat gatgaccgag
      721 aagaagagcc ccatcgacat caccgacgcg ggaacataga tccggcgata gaactttttc
      781 acgaaaaaga aatgaaggat aaatgccgca agggcgatga ggatgagcac cccggaaatc
      841 acgaagatca ggtcggtgtt cttgaacagg tccagcagca tgttcgcgct ctcctctccc
      901 acccactcgc gataagtgat gaccatgggg accaccgtcg cgatggtcgc gatgaggatg
      961 atcgacacca ggtgcgtgat gtcggcaccc cccacgaaga gcatcgtgaa caggatcggt
     1021 atgaaaacca gcgccgtgcc gaagtccggc tgcaggagaa tgatgatgat gggaacgaag
     1081 gtgagcagcg ccgggatcac gagctcgcgg aaatgcccga tctccatctc gcgcagctca
     1141 aggtacttcg ccaggatgat gatcagggag agcttcatga actccgaggg ctgtatggac
     1201 aacaccccga gcgtgatcca cgccttggag ttgcgcaccg ggcggccgaa gatcaccgtg
     1261 aacagcagca tcaggaggat cacgatgaag atgtgcaggg aataatcgcc cagccagttg
     1321 tagttgacga atgtcagggc gatcatcaac acgaacccga taatgaacca gagcagctgt
     1381 ttcttgaaca gtccgctgtt caccttctcg atgggatcga agccggcgct gtagatcatg
     1441 aggatgccga tgagcacgat gagcgccatc gcgacaacca ggagataatc tattttgtag
     1501 atctcatttc ttttcagcat tgaaatagcc ctgggacagg agtttcccga aaattcgttc
     1561 cgccacgggg acggccgtcg cggcgccggc aacgccgtat tcgaccataa ccaccaccac
     1621 gatcgcctgt tcggggggcg cgtcgaaggg cgcatagccc acgaaccagg cgtgctggga
     1681 ggcgtcctcc atccgcttgg aacgcgtctg cgccgttccc gtcttccccg ccacggggac
     1741 cttgaggccc gagagccgcg acgccgttcc ggaggcgacc accatgcgca tgccgttttt
     1801 caccacggcg agcgtgcgct gcgagagggg gatctcccgc atcttgacgg gccggaaggt
     1861 ctgcaccacc ctgcggttgt cctgggagcg cacctcccgg accaggttcg gcttgaagat
     1921 gattccattg ttcacgattc ccgagacgaa agcggccatg ccgatgggcg tcgccgaaat
     1981 gaagccctgc ccgatggaga ggttcaccgt atcgccgtcg taccaggtct ggccgaaggt
     2041 cttctgtttc cacttgcccg agggaatgaa gcccgggatc tccccgggga ggttgatgcc
     2101 ggtcttctcg cccagcccga aatagccggc gtactcgagg atgcgcgttg gcgttgtgcg
     2161 caacccaacc tggtagaaat agacgctgca cgacttgcag atggcccact ccatgttgtt
     2221 ggtgccatgg acgtcgtaac agaagaagtc cttgtccacg taccccttca gggtgtattt
     2281 cccggggcaa tacagctgcg tcgccgggtc ccagcgctcg gattcgagcg cggcgatcgc
     2341 cgtcatgagc ttgaaggtcg acgccggggg gtacttggac atgatggccc ggttgaggaa
     2401 cggccgctcc ttgtcctcca tgaggtcctt gatggtggtg acattgtcct tggagatgat
     2461 aaggttcggg tcgtaatcgg gcttggagag cagggagagg acctcgccgc tggccgcctt
     2521 catcacgata acggaaccct tggggtgcgc ttccatcgcc tcgtgcgcga tccgctggat
     2581 ctcgtaatcg atggtgagga ccatgttgct cccggacacc ggatgcagtc ccacctcctc
     2641 cacgtcggtg cgcttgcgaa cgtccacgat gcggcgaatg tagccgtcgg acccgcgcag
     2701 gaggatgtcg tgttcgctct ccaggccggc cttgccgacc ttctggtagt gccggtaccc
     2761 gaaattcctg agttttttaa attcctcgcg gctgatgctg ccggtatacc ccactgcgtg
     2821 cgcgaacata ttcccgaagt tatagacccg caccgtggag gcctcccaat cgatgaaggg
     2881 aaacttgtcc tggtgggtgg cgagcgtcac gatggagttg aaatcgacgt cctccttgat
     2941 ggtcacgcgc tcccagggat tgagtttggc gatgccctcg acggtctcat cgtacggcag
     3001 ggcgaggagt ttgcagagcc gccgcatgac cgtgcggtac tcgtccttct tcttgaagtt
     3061 ggagggcacc atggtgacat tgaacgacgg gcggttcgag acgatcacca cgctctttgc
     3121 gttcgggttg aagttgcgat cgtagatctc gccgcgcgcc gccggaatgg ggacgtagtt
     3181 ctccatgttc atccgcgact tctcccgata ctcgtcgccg cggatgagct gcaggttgat
     3241 gatctggacc acgatcacga agatgaccag cgtgacgacg cccaagaaaa agaacatgcg
     3301 catccggaat gcttccaaca ttctggtcct gagcgactgc tgaggcatgg atcagtaccc
     3361 ctctcgggac agttcgtgct cgaaaaggcg gtcgagcagg aagaagagcg gcggggcgag
     3421 gatggcgttg tagaacgcct cggggatgat gatgcttaag atgtcggaca cggccgcctg
     3481 gtgaaaaacg aacgccaaaa acagcgaaag aacgcccttc gccagcgatg cgatgaacag
     3541 caggagcagc accgtgagga tgttctcctt aaagacggca cgcccgaaca tgccgaccac
     3601 gaacccgagc gccatcttcg agaaggccag gaacccgatg ggcgaattgg agatcgcgtc
     3661 gtgcaaaagg ccgcccacga aacccgtgac ctcgccgtaa aacgatccga acgagtagcc
     3721 gaaatagatg acgatgataa acaggaggtc cggcttcacg ccggcgatcc gcagcacgtc
     3781 gaacgaggga tgcccctgaa tgaccagcga agcgatgatg aggccggctg tgatgagata
     3841 agtaaagatc atatcactcc cccagcagct ccagcatggc cttgtcgggc tccttcttga
     3901 tcacgtacac ctcttcgatc cggtcgaagt cgatgatggg ctgcaccaga acgcgctgga
     3961 aggaattcga gtccatgaga ttggtcttgg ccaccttccc caccatgagc cccgccggga
     4021 agatgccgcc ctgccccgag gtgaccacca ggtcgccgaa cttcacgacg gtggacttgc
     4081 tgatatagtc gatgaggcac aggctcgaat tcgccgtgaa gccggacaag agccccgggt
     4141 agcggctctc ctggaagacc acgccgagct tcatggtgcc ggagatgatg ggctggatgc
     4201 gcgaaacgaa ccggcggacc tcgatcacct tgcccaccac ggccttgttc tcgccgttga
     4261 aaccgatgac cggcatgttc acggcgatgc cgtcgccggt tccgcggtcg atgacgatgg
     4321 tgcggaacca attgtcgggg tccttggaga ttatccgcgc ggggatcgac tcgaactgca
     4381 cctggggccg catggacagc atcgcgcgca gtcggtcgtt ctcgcgccgg atctccgaga
     4441 gctcctcgg
//
LOCUS       DAORHF010000289         4446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20878_length_4446_cov_4.514461, whole genome shotgun sequence.
ACCESSION   DAORHF010000289 DAORHF010000000
VERSION     DAORHF010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20878_length_4446_cov_4.514461"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1677)
                     /locus_tag="PKJ16_16850"
     CDS             complement(<1..1677)
                     /locus_tag="PKJ16_16850"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HNV48713.1"
                     /translation="MIKEYATGQIRNVAIIGHGSTGKSTLFDAMLFIGGKIDKIGNAA
                     AGTLVSDFDDEEKSRKMSIKCALGFIETDDVKINIIDTPGMSDFVGDARAALQVADAA
                     ILVVDAVDGVQIETEKYWRYLNEKNIPRIIFINKMDKERANFAAITDSVAADLGTSLV
                     PLAISVGDAEQLKGVVDLVDLKAMSPKGDGRDVQVTDVPADLKGTADEARTKLMELAA
                     EGEDSLIEKFLDGGELTNDEIAIGLRNQLKAAKLHPVVCGSSQKGIGVKLLVDVIRKY
                     VPAPDPACAYAGHDMNDHAKAVSRKCLPSEPMTAVVWKTYIDQFAGRFNYLKVLSGEL
                     MPDTDVLNATRNVKERIGKLFTMVGNKQFDLPKITAGDIGVVVKLDRTATRDTLADAK
                     SPAVMPVIEVPHPVFSYAIESAKKGDEDKIGQAFARVTDENPTITYQYNAETRQTVLS
                     GMGEMQLDIILKSLKEKNKLEIITKIPRIAYRETITKKSEAQYKHKKQTGGHGQYGDV
                     YLRLAPKTRGEGYEFIDSIVGGVIPKNYIPGVEKGVKEALDEGVLARFPVV"
     gene            complement(1796..2722)
                     /locus_tag="PKJ16_16855"
     CDS             complement(1796..2722)
                     /locus_tag="PKJ16_16855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HNV48714.1"
                     /translation="MKLLVVVDDRVAGAAMRKVLERERFDFACASDAASAVAIIREQA
                     IRIAIIDWALHAADPAALCRTIRSLKKSRYVHIVAVCPRKSMGDVIDAMNAGADDYLL
                     TPFDGHELRARIDVGRRMLGSYDRLVDSQKKLLRLTREDPVTGLMNRRALFDVLLAEL
                     NRAVREGRPITVILVGLAGTRAAASGTGTPADDDLVREAGERLARTCRPYDTIGRYDA
                     EEFMAILPNTRQADAETIAVRIRDALAGVPYQAGDDSVQPAFGIGVHSISPDKRRIQK
                     SEIEHLLDGLILKVRAALRQANANGDNQIAVQ"
     gene            complement(2775..3809)
                     /locus_tag="PKJ16_16860"
     CDS             complement(2775..3809)
                     /locus_tag="PKJ16_16860"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: protein motif:HMM:NF013446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HNV48715.1"
                     /translation="MPRWLIGMCVSVAVVTLPALGMADTAAAGYRALTVEAGSEKYSG
                     YTKYQIGGKISYPNGSVGRVHFPISELRFPLEVYMAYLGVQYAATERFRTSVKAKTNI
                     NRDAGVMRDSDWGYYKLMGKDWGEKNSLDIFSESETRLGALIVDASIAYRVMKRKSLN
                     IDVGAGFLYQRFNYTISDIDQWYPSYSRYSAYIAADPELSKYSYEDHVIVHGIVATYD
                     IDYYIPYVTFSPYVNLYGKVEIKLDFMVSPWLKSKDADDHILRSKIATGDSTGMGIAT
                     RLGLRFFIEERFHVAFGFSYSFIRAEGGQTQERYEATREGPAEEIGTLENRIRNETVV
                     VTLGVGCSFF"
     gene            3950..>4446
                     /locus_tag="PKJ16_16865"
     CDS             3950..>4446
                     /locus_tag="PKJ16_16865"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HNV48716.1"
                     /translation="MFDYLIAHASIIDGTGRAPYRGAVALRGDRIEAIIPAQRGAKIA
                     LPRAKKTIDADGRALAPGFIDIHSHNDWLLPDPDHIDMLQHLMRQGVTTVVTGQCGLS
                     PAPIYTEKDDEIQSFVDLLCDRQISFDYSTMNAFFESLESKGIALNMAHLAGHATLRA
                     SLKGLL"
BASE COUNT          767 a         1416 c         1347 g          916 t
ORIGIN      
        1 gaccaccggg aagcgcgcga gcacgccctc gtcgagcgcc tccttcacgc ccttctcgac
       61 gccggggatg tagttcttgg gaatcacgcc gcccacgatg ctgtcgataa actcataccc
      121 ctcgccccgc gtcttgggcg ccagacgcag atacacatcg ccgtactgcc catggccgcc
      181 cgtctgcttc ttgtgcttgt actgcgcctc ggatttcttg gtgatggtct cgcgataggc
      241 gatccgcggt attttcgtga tgatctccag cttattcttt tccttgagcg atttaaggat
      301 gatgtccagc tgcatctcgc ccatgccgga gagcaccgtt tggcgggttt cggcattgta
      361 ctggtaggtg atggtggggt tttcgtcggt cacgcgcgcg aaggcctggc cgatcttgtc
      421 ctcgtcgccc ttcttcgccg attcgatggc gtacgagaat acgggatgcg gcacctcgat
      481 gacgggcatc accgccgggc tcttcgcgtc ggcgagggtg tcgcgggtcg cggtgcggtc
      541 gagcttcacc accacgccga tgtcgccggc cgtgatcttc ggcagatcga actgcttgtt
      601 gcccaccatg gtaaacagct tgccgatgcg ctccttgacg ttccgcgtcg cattgagaac
      661 gtcggtatcg ggcatgagct cgccggagag cactttcaaa tagttgaatc gcccggcgaa
      721 ctggtcgatg taggtcttcc agaccacggc ggtcatcggt tcggacggga ggcatttccg
      781 cgacaccgcc ttggcgtgat cgttcatgtc gtgaccggcg tatgcgcatg ccggatcggg
      841 cgccgggaca tacttgcgga tcacatcgac gaggagcttg acgccgatcc ccttctgcga
      901 cgagccgcag accaccggat gcagcttcgc cgccttgagc tggttgcgca ggccgatcgc
      961 gatctcgtcg ttggtgagct cgccgccgtc cagaaacttc tcgatcagcg agtcctctcc
     1021 ctcggccgcg agctccatga gcttggtgcg cgcctcgtcg gcggtcccct tcaggtcggc
     1081 gggaacgtcg gtcacctgca cgtcgcggcc gtcgcccttg gggctcatcg ccttcaggtc
     1141 gaccaggtcc accacgccct tgagctgctc ggcgtctccc accgaaatgg ccagcggcac
     1201 caggctcgtt cccaggtccg ccgcgacgct gtccgtgatc gcggcgaaat tcgcgcgctc
     1261 cttgtccatt ttgttgatga agatgatgcg gggaatgttc ttctcgttga gatagcgcca
     1321 gtacttctcg gtctcgatct ggacgccgtc gacggcgtcc accaccagga tggccgcgtc
     1381 ggcgacctgc agggccgctc gggcatcgcc gacgaaatcg gacatgccgg gcgtgtcgat
     1441 gatgttaatc tttacgtcgt cggtctcgat gaatcccagg gcgcacttga tcgacatctt
     1501 ccggctcttt tcctcgtcgt cgaaatcgga tacgagcgtc ccggcggcgg cgtttccgat
     1561 cttgtcgatc ttcccgccga tgaagagcat ggcgtcgaag agcgtcgact tgccggtact
     1621 gccgtggccg atgatcgcga cgttgcgtat ctggccggtt gcgtattcct tgatcatacc
     1681 ctgagcctcc cctgtgaaga atagccgaat aatgtggaaa tgagtctggt acgctaaaat
     1741 cccggtttct ttttgtaaag tattttatgg cggcctgcgc atcgacgcgc gcatttcact
     1801 gaacggcgat ctggttgtcg ccgttcgcgt tcgcctggcg aagcgccgcg cggaccttca
     1861 ggatgagccc atcgaggagg tgctcgatct cgctcttctg aatgcgccgt ttatcggggg
     1921 atatgctgtg cacgccgatc ccgaaggccg gttgaacgct gtcgtcgccg gcctgatagg
     1981 ggacccccgc gagcgcgtca cggatgcgca ccgcgatcgt ctcggcatcg gcctgtcggg
     2041 tattgggcag gatcgccatg aattcctcgg cgtcgtagcg tccgatggta tcgtagggcc
     2101 ggcaggtcct cgccagacgc tcacccgcct ccctgacgag atcgtcgtcg gcgggcgttc
     2161 cggtgccgct cgcggccgcg cgcgtcccgg cgagtcccac caggatgacc gtaatcggcc
     2221 gcccctcccg gaccgcgcga ttgagctcgg cgaggagcac gtcgaacagc gcccgtcggt
     2281 tcatgaggcc cgtgaccggg tcctcgcggg tgaggcgcaa gagcttcttc tgtgaatcga
     2341 ccagccgatc ataggagccc agcatgcggc gtccgacgtc gatgcgggcg cgcagctcat
     2401 gcccgtcgaa gggcgtgagc agatagtcgt cggcgccggc gttcatggcg tcgatcacgt
     2461 cgcccatcga cttccgcggg cacaccgcga cgatgtggac gtatcggctc ttcttgaggc
     2521 ttcggatggt gcggcacagc gcggcgggat cagcggcgtg cagcgcccag tcgatgatcg
     2581 cgatccggat cgcctgctcc ctgatgatcg cgaccgcgct cgcggcgtcc gatgcgcagg
     2641 cgaaatcgaa ccgctcgcgt tcgaggacct tccgcatggc cgcaccggca acccggtcat
     2701 cgacgacaac gagcagtttc atgtgcaata cctcgcaatg gcatttggtg cggcatcgcc
     2761 atcatcaccc ctgcctaaaa aaaggagcat cccaccccaa gggttacaac aacggtttcg
     2821 ttcctgattc tgttttccag cgtcccgatc tcctcggccg gcccctcgcg cgtggcctcg
     2881 tagcgctcct gggtttgccc cccctccgcc cggatgaacg agtaggaaaa cccaaacgcg
     2941 acatggaaac gctcttcgat gaaaaagcgc aacccaagcc tcgtcgcgat ccccatgccc
     3001 gtgctgtctc cggtcgcgat tttggagcgg aggatgtggt cgtcggcgtc cttcgacttg
     3061 agccatggcg agaccataaa atccagtttg atctcgacct tgccgtacag gttcacatag
     3121 ggcgagaagg tcacatacgg gatatagtag tcgatgtcgt aggtcgcgac gatcccgtgg
     3181 acgatcacat ggtcctcgta actgtatttg ctgagctcgg ggtccgccgc gatataggcg
     3241 gaatagcgcg aatacgacgg ataccactgg tcgatgtcgc tgatggtgta gttgaaccgc
     3301 tggtacagaa accccgcgcc gacgtcgatg ttgagcgatt tccgcttcat cacccggtac
     3361 gcgatgctcg cgtccacgat gagggcgccc aggcgcgttt cgctttcgga aaagatgtcc
     3421 aggctgttct tctcgcccca atccttgccc atgagcttgt aatagcccca gtcggaatcg
     3481 cgcatcacgc cggcgtcgcg gttaatgttg gttttcgcct tgaccgacgt ccggaagcgc
     3541 tcggtggcgg cgtactggac gccgagatag gccatgtaca cctcgagcgg aaaccggagc
     3601 tcgctgatgg gaaagtggac ccgtccgacg ctcccgttcg ggtaggagat ctttccgccg
     3661 atctgatact tggtatagcc ggaatacttc tccgagccgg cttcgacggt gagcgcgcga
     3721 tagcccgctg ccgccgtatc ggccatgccg agggcgggga gcgtcacgac cgcaacagac
     3781 acgcacattc cgatgagcca cctcggcatg ctggaccctc gttcgaatcg tttcgaccgc
     3841 gcggaacggg cgaaatcacc ttgacttttc cgttacgcgc ccgttccgtt acacagcata
     3901 cgcaaacagg ggcaatttgc aagaataata ccagcatcgg gtgctaatta tgttcgacta
     3961 cctcatcgca cacgcgagca ttatcgacgg aacggggcgc gcgccgtacc ggggcgcggt
     4021 ggccctgcgc ggcgaccgca tcgaggccat cattcccgca caacgcggtg cgaagatcgc
     4081 gctgccccgg gcgaaaaaga ccatcgacgc cgacggacgc gcgctggcgc ccggcttcat
     4141 cgacatccac tctcacaacg attggctgct tccggacccc gaccatatcg acatgctcca
     4201 gcacctgatg cgtcagggcg tgaccaccgt ggtcaccgga cagtgcggcc tctcacccgc
     4261 ccccatctac accgagaagg acgacgagat ccaatcgttc gtggacctcc tgtgcgaccg
     4321 gcagatctcg ttcgactatt ccacgatgaa cgcgttcttc gaatcgctcg agtcgaaggg
     4381 gatcgccctg aacatggcgc acctggcggg gcacgcgacg ctgcgcgcat cgctcaaggg
     4441 cctgct
//
LOCUS       DAORHF010000290         4444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_20893_length_4444_cov_5.290271, whole genome shotgun sequence.
ACCESSION   DAORHF010000290 DAORHF010000000
VERSION     DAORHF010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20893_length_4444_cov_5.290271"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..245
                     /locus_tag="PKJ16_16870"
     CDS             111..245
                     /locus_tag="PKJ16_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HNV48717.1"
                     /translation="MPLKFLLRTKKWWLVPMIVVLLLIGALIVLTSGTAIAPFIYTLF
                     "
     gene            256..687
                     /locus_tag="PKJ16_16875"
     CDS             256..687
                     /locus_tag="PKJ16_16875"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HNV48718.1"
                     /translation="MKDAKTDKRARDLETIGVIALALFIIHFILRYKSPSAIGDGLFA
                     MLSANALLLSGILVLALGLASRTVAGLISDLWLKFSHVLGSVTNRIILSVIFFVFLTP
                     IALLYRLFAANPLQLSREKSGASMYHERNYTFRKEDFEKMW"
     gene            717..1622
                     /locus_tag="PKJ16_16880"
     CDS             717..1622
                     /locus_tag="PKJ16_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HNV48719.1"
                     /translation="MNTPILFIIFNRPETTRLVFERIRQAKPTRLFVSADGPRSSVPG
                     EAERCREARAIVERVDWDCEVKTFFKDSNCGCRVMVSSAITWFFDNVEEGIILEDDCL
                     PDMSFFPFCAELLERYRDDSRIYMISGNNFQFGIKRGTGSYYFSKTNHIWGWASWRRA
                     WRQYDVALASFPEFKRNNIIRNIFSDKKVRTQCMRNFQLVFNKKLDTWDHQWGYAILT
                     NNGLSIMPNDNLVSNIGFTAGATHTTTGGNIFADITTEPINEIIHPSFVVPDLEADKY
                     ERDIYRKAIPFTRKVRNKLSVLMER"
     gene            1623..2756
                     /locus_tag="PKJ16_16885"
     CDS             1623..2756
                     /locus_tag="PKJ16_16885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011373048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV48720.1"
                     /translation="MKVLLLADASVVHTLKWARSLSERGIEVVIFGMVDFDRSIYDGY
                     NNIEIVIYKRETELLRSGAGNVRKLAYLKALPVIRRLIREHKPDIVHAHFATSYGLLG
                     ALAGFRPFIVSVWGMDAFDFPKISIVHRLVFKFILSRADRVLSTSNVMARETQQYTGK
                     TVITTPFGIDLKRFRPAPVKSLFGKKDLVIGTIKDLEEKYGIEYLIRAFAMLKGRHPK
                     LSLKLLIVGGGSQDAFLKKLCADLGITSHVAFTGMIPYDEVVRYHNMIDIYVAVSVYQ
                     SESFGVAIIESSACEKPVVVSNMGGLPEVVDDGVTGFVVPARDPDATAAAIERLVLDK
                     KLRAKMGTAGRRRVMELFDWKVNVDQMVSIYDDVLREKKKSYN"
     gene            2787..3962
                     /locus_tag="PKJ16_16890"
     CDS             2787..3962
                     /locus_tag="PKJ16_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HNV48721.1"
                     /translation="MTIRDVLVQIYIFFITVRDFFLYRLLNPLATRTRPTRSAGKELL
                     VVRCDAIGDYILFRDFLAEIKQSARFSGYRITLCGNALWRDVAERFDRAIIDDFIWLE
                     RKRFVKSARYRLGILNRVRARHYTCVINPAFSRDYFVDDEIVRYASANEKIGSSGDDA
                     NTFPLLKRLADRYYTSLIEATGDTMFEFNRNREFSQKLLGTPSAVDRPRLDVSAIKPP
                     IELPGDFVVLFPSASIKYKRWDTANFAVIADHVAERYGLSIVVAGAPSDGTYAREIAS
                     RARCPFIDITGKTTIPELAAVLAKTRLLVSNDTVAVHLATAVGATSICISQGGRFGRF
                     VTRSREVFPENYFIYPDEIMRDIDDPKLGEKYKYGSGLSINGIRPERVVALIDTILA"
     gene            complement(4217..>4444)
                     /locus_tag="PKJ16_16895"
     CDS             complement(4217..>4444)
                     /locus_tag="PKJ16_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48722.1"
                     /translation="LRATRADPSVRPVAAMPAGKAGGANAALFAVSILASGDAALAAK
                     IDEYRKKTEKKIKERNEKLNAVGYAEYIKGL"
BASE COUNT          948 a         1329 c         1220 g          947 t
ORIGIN      
        1 cttctcgcca gacgtcgccg ccgacgatcc gcatcccaat gccctggctc acaaagaaat
       61 ggctggattc ataaccgcat ttttgaaaac ccggttcggt ggtgcaaatt atgcccctta
      121 agtttctgtt gaggaccaag aagtggtggc tggtgcccat gatcgtagtg ctgctgctca
      181 ttggagcgct catcgtgctc accagcggta cggcaatcgc gccgttcatc tacacgcttt
      241 tctaggaccg aacacgtgaa ggatgcgaaa accgacaagc gggcacggga cctcgagacc
      301 atcggggtga ttgcgctcgc gctgttcatc atccacttca ttcttcgata caaaagccca
      361 tcggccatcg gcgatgggct tttcgcgatg ctctcggcga atgcgctgct attgtccggg
      421 attctcgttc tcgcgctcgg gctggcgtcg cgaaccgtcg ccggcctgat atccgatctc
      481 tggctcaagt tctcgcacgt gctgggatcc gtcaccaacc ggatcatcct gtccgttatc
      541 tttttcgtct tcctgacgcc gatagcgctc ctctatcgcc tctttgccgc aaaccccctt
      601 cagctttcgc gcgaaaagtc aggcgcgagc atgtaccacg agcggaatta cacgttccgg
      661 aaggaagact tcgaaaagat gtggtgatac ggcgatccga gtcatctgaa cacaccgtga
      721 acacccccat cctcttcatc atcttcaacc gccccgagac cacgcgactt gtcttcgaac
      781 gaataaggca ggcaaagccc acgcgcctgt tcgtctccgc cgacggtccg cgctcgagcg
      841 tacccggcga agcggagcgc tgtcgcgagg cgcgcgcaat cgtcgagagg gtcgattggg
      901 actgcgaggt gaagaccttt ttcaaggatt cgaactgcgg gtgccgcgtg atggtgagct
      961 ccgcgataac gtggttcttt gacaacgtcg aggaagggat catcctggag gacgattgcc
     1021 tgccagacat gtcgttcttt cccttctgtg ccgaactgct tgaacgttac cgcgacgaca
     1081 gccgcatcta catgatcagc ggcaacaatt tccagtttgg catcaagcgg gggacgggga
     1141 gctactattt ttcgaagacc aatcacatct ggggatgggc ctcgtggcgg cgcgcgtggc
     1201 ggcaatacga cgttgctctc gcaagctttc cggaatttaa gcgaaacaac atcattcgaa
     1261 atattttttc ggacaaaaag gtacgtaccc aatgcatgcg caattttcaa ctcgtcttca
     1321 acaagaagct cgacacttgg gatcatcaat ggggatacgc catcctgacg aacaacggac
     1381 tatcgattat gccgaacgac aatctcgtct cgaatatagg ctttactgcc ggcgcaacgc
     1441 acacgacaac cggcggcaat atcttcgctg acataacaac tgagccgatc aatgagatca
     1501 tccacccgtc tttcgtggtt ccggatctcg aagctgacaa atacgagcga gacatttacc
     1561 gcaaagccat tccgttcacc cgcaaggtgc gaaacaaact ctccgtgctc atggaacgct
     1621 aaatgaaggt cctcctcctc gccgacgcat ccgtcgtcca cacgctcaaa tgggcgcgct
     1681 cactctccga gcgcggcatc gaggtagtga tattcggcat ggtcgatttc gaccggtcga
     1741 tctacgacgg ctacaataat atcgagatcg taatatacaa gcgcgagacc gagctcctgc
     1801 gcagcggtgc cggaaacgta cgcaagctcg cctacctgaa ggcgctcccc gtcatccggc
     1861 ggctgatccg cgagcacaag ccggatatcg tacacgcgca tttcgcgacg agctacgggc
     1921 tcctcggcgc cctcgcggga tttcgcccgt tcatcgtgtc ggtatgggga atggacgcct
     1981 tcgacttccc gaaaatttcc atcgtccacc gtttggtctt caaatttatt ttatcgcgtg
     2041 cggaccgggt gctctccacg agcaatgtta tggcgcgcga gacgcagcaa tacaccggca
     2101 aaacggtcat caccacgcca ttcggcatcg acctgaaacg cttccggccc gctcccgtga
     2161 aaagcctttt cgggaagaag gaccttgtca ttggcaccat caaggacctc gaggagaaat
     2221 acggcattga atatctcatc cgcgcattcg cgatgctcaa gggacgccat ccgaagttat
     2281 cgctcaagct gctcatcgtc gggggcggat cgcaggacgc ctttctgaaa aagctgtgcg
     2341 ccgatctcgg cataacctcg catgtcgcgt ttacggggat gattccctac gacgaggtcg
     2401 tccgctatca caacatgatc gacatctacg tcgcggtgtc ggtttaccag agcgagagct
     2461 tcggggtggc catcatcgaa tcgtcggcct gcgagaagcc ggtagtcgtg tccaacatgg
     2521 gcggccttcc ggaggtcgtg gacgatggcg tcaccggctt cgtcgtgccg gcgcgcgacc
     2581 cggacgcgac tgccgccgcg atcgaacgat tggtgctgga caaaaaactc cgcgcgaaga
     2641 tgggaacggc cggacggcgg cgggtgatgg agctcttcga ctggaaagtg aacgtggacc
     2701 agatggtgtc gatctatgac gatgtccttc gtgaaaagaa aaaatcgtac aactagaaca
     2761 ctacgccctc acacacaacg aaatccatga caatccggga tgtcctcgta cagatatata
     2821 ttttttttat taccgtccgc gatttttttc tctatcgtct tctgaatccc ctcgcgactc
     2881 gaacgcgccc gacgcgctcg gcgggaaagg agctcctcgt cgtccgctgc gatgccatcg
     2941 gcgactatat tctttttcgc gattttctcg ccgagatcaa gcagagcgcg cgcttttcag
     3001 gctatcgcat aacgctctgc ggaaacgcgc tctggcgcga tgtggcggag cgcttcgatc
     3061 gcgcgatcat cgacgacttc atctggctcg agcggaagcg attcgtaaag agcgcgcgct
     3121 accggctggg aatcctcaat agagtgcggg cacgccacta cacatgcgtc atcaaccccg
     3181 cattctcgcg tgattatttt gtggacgatg agatcgtccg atacgcatcc gcgaacgaga
     3241 agataggcag cagtggcgac gatgcgaaca ccttccctct attgaaacgc ctcgccgatc
     3301 gatactatac gtcgctaatc gaggcgacag gcgatacgat gttcgaattc aatcgcaatc
     3361 gggaattctc gcaaaaactg cttgggacgc cttcggcagt cgaccggccg cggcttgatg
     3421 tgagcgccat caagcctccg atcgaattgc cgggggattt cgtcgttctg ttcccgagcg
     3481 cgagcatcaa atacaagcgt tgggataccg cgaacttcgc cgtcatcgcc gaccatgtgg
     3541 ccgagcgcta cgggctttcg atcgtcgtcg cgggcgcgcc gtctgacgga acctacgcgc
     3601 gcgagatcgc gtcccgggcg cgctgcccct tcatcgacat caccgggaaa accaccatcc
     3661 cggagctcgc ggctgtgttg gctaagacgc gtctgctggt atccaacgac acggttgccg
     3721 tgcacctcgc gaccgcggtg ggcgcgacgt cgatctgcat aagccagggc ggacggttcg
     3781 ggaggttcgt gactcgatcg cgcgaggtgt tcccggagaa ctatttcatt tatccggacg
     3841 aaatcatgcg ggacattgat gatcccaaac tgggcgagaa atataaatac gggtcggggc
     3901 tttcgattaa cggcatacgg ccggaaagag tcgtagcgct gattgacacg atactagcgt
     3961 agttacgcgc ttaccacctc atcccccccg gcccgccgag cgttcattgg gcctcctgcc
     4021 cacgctcggc actgcaacat cctgttggga gccttctccc ataggagaag ggggagaact
     4081 caagaactag ccctttccta agaagccctc tcctatggga ggcagcatat cgaacatcct
     4141 tattcgatac tgcatacgcg acctcctgtc gctaagggcg cggcagagcc ggggtgaggt
     4201 aacgtcagcg aatcgactac aaccccttaa tatactccgc atacccgacc gcgttcagct
     4261 tctcgttgcg ttccttgatc ttcttctccg tctttttccg atactcgtcg atcttcgcgg
     4321 cgagcgcggc gtcgcccgag gcgaggatgg agacggcgaa gagcgccgca ttcgctccgc
     4381 cggccttccc cgcgggcatg gccgcgaccg ggcgcacgct cggatcggcg cgggtcgctc
     4441 gcag
//
LOCUS       DAORHF010000291         4360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21510_length_4360_cov_5.528455, whole genome shotgun sequence.
ACCESSION   DAORHF010000291 DAORHF010000000
VERSION     DAORHF010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21510_length_4360_cov_5.528455"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PKJ16_16900"
     CDS             <1..628
                     /locus_tag="PKJ16_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48723.1"
                     /translation="PQRRAQRREQRRPAEPTPPEHTREELIERFVALRESAKDVLYRY
                     FQYNLNNIARRDEPKNKGTFNEVVYSLRKNFHSIKRLATLTKDAELLEHFSVFTEMLF
                     NFYRSSECLTILDSYNSRLDIEAYRLYKKGDDALHASHREIFYDRHNRGSFKKDIAVT
                     HLVKAEQYFSQTMKTFPDSSWAVETGIKLEYCQSLKKYLILFFSDAAA"
     gene            625..1194
                     /locus_tag="PKJ16_16905"
     CDS             625..1194
                     /locus_tag="PKJ16_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HNV48724.1"
                     /translation="MNLADSLAILGIAVGLAMDAFSVCIAAGMIIDRPTARHYFRLAF
                     HFGLFQFMMPILGYFAGRYLESIIKDYDHWIALAILTVIGAKMIRESLSDDDRRIERD
                     PSRGWTLIVLAVATSIDALAVGLSLGLLGSGILVPSVIIGVVCALFSVIGISIGKRVG
                     ALFGRRVEMIGGVILIAIGIRIAVSHLFP"
     gene            1393..2082
                     /locus_tag="PKJ16_16910"
     CDS             1393..2082
                     /locus_tag="PKJ16_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48725.1"
                     /translation="MRKTTIAIMCLLLLPLSLGAEDYQYQFFKEKDQKKHLPFSDYIP
                     KVRLHYKTVPHYLEDYYELYGKKQYYNENTLRMNIARLTTALECKFRHPSEALVPVST
                     EEEYRKYRNLMFMHIHLLIMRSHMRIASRYDKRVIKFYNLDFAKEIAESLDIARTHYS
                     AAIPHWEHAQRYARSASEIKLSTELGFMESERYRIIKGETDFGKIIGNYLSLLDRKKE
                     TLDAALAEAKK"
     gene            2198..3277
                     /locus_tag="PKJ16_16915"
     CDS             2198..3277
                     /locus_tag="PKJ16_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48726.1"
                     /translation="MSEPAPDKPVRTALLITDDKAASITIRNIVKKYGVEINTSYLNL
                     NSTAMIKKIILKTGNTAFMRADLYKLITEIGVPFLVSLDYTIDLGLDHALDPDRRKIL
                     RTLLISYIILAKAQGLEHLQANFMVLYRSAQAAHVRPFEANPAGILDILYTGNTKANE
                     IINELKGDPARFNRLFQIRFVNISQTQGEIDAAIRAFITGLETREKLFEMIARKKNTD
                     LEQEHKTAAQIVYRIDRDTVYVDGETSEIPKGSPYLDFSINEFYAVGSWEQLNQLAVA
                     DKLKKAITGGIGEKSFKPADPIVLNLGDDCLIDGATANSLARLFVQELGNYKDVKIYV
                     NSDNAIALEKSKGFIIIKNLIRHSY"
     gene            complement(3316..>4360)
                     /locus_tag="PKJ16_16920"
     CDS             complement(3316..>4360)
                     /locus_tag="PKJ16_16920"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HNV48727.1"
                     /translation="FYSVVGGWTIGYIVKSVSGGMSSLTNVETAKNAFAQFIHNPYEM
                     IAYHFLFMAACAFIVIRGVKNGIEWWSKVLMPLLFILLLVLIVKGVSMKGGMAGVSFF
                     LNPDFSKLTVDAVLSALGQSFFSLSLGMGAMITYGSYLSRKERILQSAMYIVLLDTLV
                     AILAGLAIFPAVFAMEMAPDKGPGLVFHVIPAVFANMPYGQVFAVVFFGLLFIAALTS
                     GISLLEVVVAWAGDERGWSRTKAVLILGGAIFAMGVPSALSFNVLEGNTVFFGKTFFD
                     FIDALTSNYMLPIGGFFIAVALGWKYGLDKTIHELDPDTRIISLKELWAFTIKFVSPL
                     LVALVFIVNFLGR"
BASE COUNT          981 a         1446 c         1167 g          766 t
ORIGIN      
        1 gccccagcgc cgcgcccagc gacgcgaaca gcgccgcccc gccgagccaa cgccgcccga
       61 gcatacgcgc gaggagctta tcgaacggtt cgtcgcgctc cgggaatcgg cgaaggacgt
      121 cctctaccgg tatttccagt acaacctcaa caacatcgcg cgacgcgatg agcccaaaaa
      181 caaggggacc ttcaacgagg tcgtctattc cctgcgaaag aatttccact cgatcaagcg
      241 ccttgcgacg ctcacgaagg acgccgagct tctggagcac ttttccgtat tcacggagat
      301 gctgttcaac ttctaccgct cctccgaatg cctcaccata ctcgactcgt acaacagccg
      361 tctcgacatc gaggcctacc gtctgtacaa aaagggggat gatgcgctgc acgcgtcgca
      421 ccgcgaaata ttttacgacc gccacaaccg cggctccttc aagaaggaca tcgccgtgac
      481 gcacctggtc aaagcggagc aatacttttc ccaaaccatg aagaccttcc ccgattcgtc
      541 ctgggcggtc gagaccggga tcaagctcga atactgccag tcgctcaaga agtatcttat
      601 cctctttttt tccgatgcgg cggcatgaac ctggccgatt ctctcgccat cctcggcatt
      661 gccgtgggcc tggccatgga cgcgttctcc gtctgcatcg ccgccggcat gatcatcgac
      721 cgacccaccg cccggcacta cttccgcctt gcgttccatt tcgggctctt ccagttcatg
      781 atgccgatcc tgggctactt cgccggccga tacctcgaat cgatcatcaa ggactacgac
      841 cactggattg cgctggccat cctcaccgtg atcggcgcaa agatgatccg cgagtcgcta
      901 tccgacgacg accggcgcat cgaacgcgac ccctcgcgcg gatggaccct gatcgttctc
      961 gccgtcgcga cgagcatcga tgcgctcgcg gtgggcctga gcctgggcct cctcggctcg
     1021 ggcattctcg tgccgagcgt catcatcggc gtggtgtgcg cgctgttttc cgtgatcggc
     1081 atctcgatcg ggaagcgcgt cggcgcgctc ttcggccggc gggttgagat gatcggcggc
     1141 gtcatcctca tcgcgatcgg gatcaggatc gccgtttcgc accttttccc ctgacgcggc
     1201 ccggacgggc gcccaacgcc ggccatccga ccggatcata ctttgtgctt taaaacaaac
     1261 gcctcccgcg agatgcccgt tcggaaccgt gcggtttcct ccctcatttt ttcccgcaaa
     1321 acggccgata ggagaagaag catcgtgccg cccttggcgc tgggagggcg cgcgtttccg
     1381 gtcatcgggt gtatgcgaaa aacgactatc gccatcatgt gcctgctcct ccttccgctc
     1441 tcgctcgggg ccgaggacta ccagtatcag ttcttcaagg aaaaggacca gaaaaaacac
     1501 ctgcccttct cggactacat tcccaaggtg cgcctgcact acaagaccgt cccccactat
     1561 ctcgaggact actacgagct ctacgggaaa aagcagtact acaacgagaa caccctccgc
     1621 atgaacatcg cgcggctgac caccgcgctg gagtgcaagt tccgccaccc ctccgaggcg
     1681 ctggtcccag tttcgaccga ggaggaatat cgcaagtacc ggaacctgat gttcatgcac
     1741 atccacctgc tcatcatgcg cagccacatg cggatcgcat cgcgctacga caagcgcgtg
     1801 attaaattct acaacctcga tttcgccaag gagatcgccg agagcctgga catcgcccgg
     1861 acccactact ccgcggcgat tccccactgg gagcacgcgc agcgctacgc ccgcagcgcg
     1921 agcgagatca agctctccac cgaactgggg ttcatggagt cggagcgcta ccgcatcatc
     1981 aagggggaga ccgatttcgg gaagatcatc gggaactacc tctcgctcct ggaccggaaa
     2041 aaagagacgc tcgatgcggc cctcgccgag gcgaaaaagt aacgcgatac cgccccgcga
     2101 ccgcgtcgct tccgctattc ccgttgacaa tcccgcaccc attgaacgac aatgaccctc
     2161 ggcccggagg agtgctccgg gaccgcacaa tcaacccatg agcgaaccag caccagacaa
     2221 acccgtccgc accgcgcttc tcataaccga cgacaaggcg gcgtcgatca cgatccgcaa
     2281 catcgtgaag aaatacggcg tcgagatcaa taccagctac ctcaacctga actcgacggc
     2341 catgatcaag aagatcatcc tcaagacggg caacaccgcc ttcatgcgcg cggacctcta
     2401 caagctcatc acggaaatcg gcgtcccttt tctggtttcg ctcgactaca ccatcgacct
     2461 cgggctcgac cacgcgctcg accccgaccg gcggaagatc ctgcgcacgc tcctcatctc
     2521 gtatatcatc cttgcgaagg cgcaggggct ggagcacctg caggcgaatt tcatggtcct
     2581 ctaccgcagc gcccaagcgg cgcacgtacg ccccttcgag gcaaatccgg ccggtatact
     2641 cgacatcctc tacaccggca acaccaaggc gaacgagatc atcaacgagc tcaagggaga
     2701 tcccgcgcgc ttcaaccggc tcttccagat ccggttcgtg aacatctcgc agacccaagg
     2761 ggagatcgac gccgcgatcc gcgccttcat taccgggctt gaaacgcgcg agaagctctt
     2821 tgaaatgata gcgcgcaaga aaaacaccga cctcgagcag gagcacaaga ccgccgcgca
     2881 gatcgtctat cgcatcgacc gcgacacggt gtacgtcgac ggcgagacga gcgagatccc
     2941 caaggggtcg ccctatctcg acttctccat caacgagttc tatgccgtgg gttcgtggga
     3001 gcagctcaat cagctggcgg tggcggacaa gctcaagaag gcgatcaccg gcggaatcgg
     3061 cgaaaaaagt tttaagcccg cggaccccat cgtcctcaac ctcggcgacg actgcctgat
     3121 cgacggcgcg acggcgaact cgctcgcgcg gttgttcgtg caggagctgg gaaactataa
     3181 ggacgtgaag atatacgtga acagcgacaa tgcgatcgcg cttgaaaagt cgaaggggtt
     3241 catcatcatc aagaacctca tcaggcactc gtactgatcc gccgcccgcc gacgcgcagc
     3301 ggcgatcccc ccgtcctacc tccccagaaa gttgacgatg aacaccagcg ccaccaagag
     3361 cggcgagacg aacttgatgg tgaaggccca cagctccttg agcgatatga tccgggtgtc
     3421 gggatcgagc tcgtggatcg tcttatcgag accgtacttc catccgagcg cgacggcgat
     3481 gaaaaaaccg ccgatgggca gcatgtagtt cgacgtgagc gcgtcgatga aatcgaagaa
     3541 ggtctttccg aaaaacaccg tattcccctc gagcacgttg aacgacagcg ccgaggggac
     3601 gcccattgcg aagatcgctc cgcccagaat gagcaccgcc ttcgtgcggc tccagccccg
     3661 ctcgtcgccc gcccaggcga ccaccacctc gagcagcgag atcccgctgg tgagcgccgc
     3721 aatgaacaac agcccgaaga acacgaccgc gaacacctgc ccgtagggca tattggcgaa
     3781 gacggccggg atcacgtgaa acacgagccc cggtcccttg tccggcgcca tctccatcgc
     3841 gaagacggcg ggaaagatgg ccaggccggc gaggatggcc accagggtgt ccagcagcac
     3901 gatgtacatc gccgattgca ggatccgctc cttgcgcgac agatagctgc cgtaggtgat
     3961 catcgccccc atgccgagac tgaggctgaa gaaggactgg cccagcgccg agaggaccgc
     4021 atccacggta agcttcgaaa agtcgggatt cagaaaaaac gaaacccccg ccatcccgcc
     4081 tttcatcgac acgcccttga cgataaggac cagcagcaaa atgaacagga gcggcatgag
     4141 caccttgctc caccattcga tgccgttttt cactccccgt atcacgatga aggcgcaggc
     4201 ggccataaac aagaaatgat acgcgatcat ctcgtacgga ttgtggatga attgggcgaa
     4261 tgcgtttttc gccgtctcca cgttcgtcaa cgacgacatc cccccgctca cgctcttgac
     4321 gatatatccg atcgtccagc cgcccacaac gctgtaaaaa
//
LOCUS       DAORHF010000292         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21657_length_4341_cov_5.735884, whole genome shotgun sequence.
ACCESSION   DAORHF010000292 DAORHF010000000
VERSION     DAORHF010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21657_length_4341_cov_5.735884"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="PKJ16_16925"
     CDS             complement(<1..451)
                     /locus_tag="PKJ16_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48728.1"
                     /translation="MTLYRIGLVVLILLFAVGFALVFKSVHLPARVRRAEEFMNEEDL
                     SRASAIVKSILDKKKDYVPARYVRARILMKQSQFMLAISELNAILGIPDFNKHVSELD
                     IHYLLADLYSATQQFPKEIDEYREILKFKPEDVKANHRLGHAQYKQKK"
     gene            711..2618
                     /locus_tag="PKJ16_16930"
     CDS             711..2618
                     /locus_tag="PKJ16_16930"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48729.1"
                     /translation="MKNRCFVPFVTVLIPVALSLSGCTSAPPVPTKPAAEPTASREIV
                     ADWHLYSMGLYYKSTKNYTTAIKYFLDAAAHGVALDLVYEKLAECYSLLFDYDTAINY
                     AKLSIEKNPSSPAPYLLLYRIYVNLNNHQQAAEALERLIAVDPEPVEVHFRLGVHYYT
                     RMKNYDRALVSFRAILDIAASKHVEDRFLEYAHYYIGYIHYNKGQIDASIEHFARSAD
                     INPGNAQTLNILIILLMHSTQIDRAAGYAAQFLERYPDNARMNAVMGQILYLRNSPRA
                     KSYLRRGMGDASPYGITARGLYYELLRRDDRALASIKKSLENNPGAVAAHVALAKLSL
                     RKHKKRVALSEYFTAGILLYKAKQYSDARTYLTKSLALDDGIPETYIYLGKISEETGN
                     RSLAIVHYKKANQLQPRSDIYSHLGYLYSQRNDFAESMRNFDRAIAMEPKNPKSYFIK
                     GIVLSDRKDYPRAEQEFRTAVSLDGNDTYYFYLATVQEKQRKLDEAMDSLRRALAVNA
                     ENPRACNYLGYLYADNNMRLDESIDLIRRALKHEPRNGAYLDSLGWAYYRKGDYQRAL
                     KKLLEAQRLLERDKASDPVVYDHIADTYKMIGDREKALEYWRKSLHLEKNPAIETKIR
                     EIEALIKQQGN"
     gene            2625..3455
                     /locus_tag="PKJ16_16935"
     CDS             2625..3455
                     /locus_tag="PKJ16_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48730.1"
                     /translation="MNSVARMTRGVAAAVAVVCMAGCASGNRAADDQRASLGDAAVKA
                     LVQRVRDLNAGAPDRFVASFTVEGTMHNRKNFKSLGSAMFLRDPLRMKVIFTDLIFKS
                     PISMLVRNGDELRFYFPAERKLVIDSAAKIDLNRYIEIDVDFLFLSQIASGRVGTIEN
                     FKASGGADATGDPKAAGTTYLILENDRFYQTIAFRNNLPEKILLVRKSNNHRFEFHFE
                     APAYQNNRFTCRSIRFISLARKDVIRLTFNRINYNVAIDPDGVTDIPMDRKTETIRVN
                     "
     gene            complement(3517..3783)
                     /locus_tag="PKJ16_16940"
     CDS             complement(3517..3783)
                     /locus_tag="PKJ16_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002786879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HNV48731.1"
                     /translation="MISIAASNVVDVEPAACRVWVDARTVFVELTDGRILGFPADRFR
                     ILSKATDEQLRKVVLRLNGFALRWEELDEDISVPGIISGIFQLP"
     gene            complement(3761..4000)
                     /locus_tag="PKJ16_16945"
     CDS             complement(3761..4000)
                     /locus_tag="PKJ16_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HNV48732.1"
                     /translation="MPTVLRIGPFRFHFYSNEGGEAPHIHVESSEGECKFWLDPVRLA
                     RNKGLSPHVVREIEKLVYEHLEYLKERYYDFHRGQ"
     gene            complement(4053..>4341)
                     /locus_tag="PKJ16_16950"
     CDS             complement(4053..>4341)
                     /locus_tag="PKJ16_16950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HNV48733.1"
                     /translation="EGFGFPACRRPWPRPRVIASDGGALPEVVGDAGVIVPARDSYAL
                     AEAIHALLADPKKMKSLGAAGQARVAEHFSWEAAVRRMVGVYERVLMHASV"
BASE COUNT          985 a         1413 c         1181 g          762 t
ORIGIN      
        1 atttcttctg cttgtactgg gcgtgcccca gccgatggtt ggccttcacg tcctcgggtt
       61 tgaacttgag gatctcacga tactcgtcga tctccttggg gaactgctgg gtggcgctgt
      121 agagatcggc cagcaggtag tggatgtcga gctcgctcac gtgcttgttg aaatcgggaa
      181 tgcccaaaat ggcgttgagc tccgagatgg ccagcatgaa ctgcgactgc ttcatgagga
      241 tgcgcgcccg cacatagcgc gccgggacat agtccttctt cttgtccagg atgctcttga
      301 cgatggcgct cgcgcgggag agatcctcct cgttcatgaa ctcctcggcg cgacgcaccc
      361 gcgcaggcag gtgcacggac ttgaagacga gcgcaaaccc caccgcaaac agcaggatga
      421 gcaccaccag cccgatccgg tataatgtca tatcacccat aggtcacctg cacgaaggcc
      481 cgcgcgccgc caatgcgcgc aatggcccat aaacatagtc agtggagcga tatccgtcaa
      541 caataattcg ccgcatggcg gcaacggccg cgaatatcgg cgaagcgcca tcacccccac
      601 acccatgtga agaaataccc tgtgagccgc aaatccattt gacaagaacg cattgtattg
      661 ctatactatg agggctggga accaaaagtg aacttgtgca caaaaatgta atgaaaaatc
      721 gatgtttcgt accgttcgtg accgtgctca ttcccgtcgc cctttcccta tcgggctgca
      781 cgtccgcccc tccagtgccc acgaagcccg ccgcggaacc gaccgccagc cgggagatcg
      841 tggccgactg gcacctgtac agcatggggc tgtattacaa atcgaccaag aactacacca
      901 ccgccatcaa gtacttcctc gacgccgcgg cgcacggggt cgcgctcgat ctggtctacg
      961 aaaagctcgc ggaatgctac agccttctct tcgactacga caccgcgatc aactacgcga
     1021 agctgtccat cgagaagaac ccgtcctcgc cggcgccgta tctgctgctg tatcggatct
     1081 acgtgaacct gaacaaccat cagcaggcgg cggaggccct cgagcggctc atcgcggtgg
     1141 accccgagcc ggtggaggtg catttccgcc tcggcgtcca ctactacacg cgcatgaaga
     1201 actacgaccg cgccctggtg agctttcgcg ccatcctgga catcgccgcc tccaagcacg
     1261 tggaggaccg cttcctggag tacgcgcatt actacatcgg ctacatccac tacaacaagg
     1321 gacagatcga cgcgtcgatc gagcacttcg cccgttcggc cgacatcaat cccggaaacg
     1381 cgcaaacgct caacatcctg atcattctcc tgatgcacag cacgcagatc gaccgggcgg
     1441 ccggatacgc cgcgcagttc ctggagcgct atcccgacaa tgcgcgcatg aacgccgtca
     1501 tggggcagat cctctacctg cgcaacagcc cgcgcgcgaa atcatacctg cgccgcggca
     1561 tgggcgatgc gtcgccctac ggcatcacgg cgcgcgggct gtactacgag ctcctgcgtc
     1621 gcgacgaccg cgcgctcgcg tccatcaaaa agtcgctcga gaacaatccg ggcgccgtcg
     1681 ccgcccatgt cgcgctggcg aaactctcgc tgcgcaagca caagaagcgc gtggccctga
     1741 gcgagtactt cacggcgggc atcctcctgt acaaggccaa gcagtattcc gatgcgcgca
     1801 catatcttac aaaatcgctc gcgcttgatg atgggatacc cgaaacctac atctacctgg
     1861 gaaagatttc ggaggagacg gggaaccgct cgctggccat cgtccactac aagaaggcca
     1921 accagctcca gccgcgctcg gacatctatt cgcacctggg atacctctac agccagcgca
     1981 acgacttcgc cgaatcgatg cgcaacttcg accgcgccat cgccatggag ccgaagaacc
     2041 cgaaatcgta tttcatcaag gggatcgtcc tgtcggaccg caaggactat ccccgcgccg
     2101 agcaggagtt ccgcacggcc gtgtcgctgg acggcaacga cacctactac ttctacctgg
     2161 ccacggtgca ggagaagcag cgcaagctcg acgaggcgat ggactcgctg cgcagggcgc
     2221 tcgcggtgaa cgcggagaac ccgcgcgcgt gcaactatct gggctatctc tacgccgaca
     2281 acaacatgcg cctggacgag tcgatcgacc tcattcggcg cgcgctgaag cacgaaccgc
     2341 gcaacggggc ctacctcgac tcgctgggct gggcctacta ccggaagggc gactaccagc
     2401 gcgcgctcaa gaagctcctc gaggcgcagc gcctcctcga gcgcgacaag gcctcagatc
     2461 cggtggtcta cgatcatatc gccgacacct acaaaatgat cggcgaccgg gaaaaagcgc
     2521 ttgaatactg gcgcaaatcc ctgcacctgg aaaaaaatcc cgcgatcgaa accaagatcc
     2581 gggagatcga agcgctcatc aaacaacagg ggaactaatc gcatatgaac agcgtggctc
     2641 ggatgacccg cggcgtcgcc gcggcggttg cagtcgtgtg tatggcggga tgcgcctccg
     2701 gaaaccgcgc cgcggacgat cagcgcgcct cgctcggcga tgcggcggtg aaggcgctcg
     2761 tacagcgggt gcgggacctc aacgccggcg cgccggaccg cttcgtggcc agcttcaccg
     2821 tcgagggcac catgcacaac cggaagaact tcaagtcgct cgggagcgcc atgttcctgc
     2881 gcgatccgct gcgcatgaag gtcatcttca ccgacctcat cttcaagagc cccatctcca
     2941 tgctggtgcg aaacggcgac gagctgcgct tctactttcc cgccgagcgc aagctcgtga
     3001 tcgacagcgc cgccaaaatc gacttgaacc gctacatcga gatcgacgtc gactttttgt
     3061 tcttaagcca gatcgcaagc gggcgcgtgg ggacgatcga gaacttcaag gcatcgggcg
     3121 gcgcagacgc gacgggcgat ccgaaggcgg cggggaccac ctatctcatc ctcgagaacg
     3181 accgctttta tcagaccatc gccttcagga acaatctccc ggagaagatc ctcctggtca
     3241 ggaaatcgaa caaccaccgc ttcgagttcc actttgaagc gccggcatac cagaacaacc
     3301 gcttcacctg ccggtcgatc cggttcattt cgctggcccg caaggacgtg atccggctca
     3361 ccttcaaccg gataaactac aacgtggcca tcgatcccga tggcgtcacc gacatcccca
     3421 tggaccggaa aacggagacc atccgggtca actgagaaga cgtccagctt tactgtacat
     3481 caactcggtg caataaaaca tacgtttcac taaaacttat ggcagctgga aaatacccga
     3541 gatgattccc ggtacggaga tgtcttcatc gagttcctcc caacgtaacg caaagccatt
     3601 aagccgcagg acaaccttac gcaactgctc atccgtcgct ttgctaagaa ttcggaaccg
     3661 gtcggcgggg aagcccagta tccgcccatc agtaagctcg acaaaaaccg tacgcgcatc
     3721 gacccacacc ctgcaggcgg cgggttcgac atccacgacg ttactggccg cgatggaaat
     3781 cataatatcg ctccttcaag tactcaaggt gctcatagac cagcttttct atctcacgca
     3841 ctacatgtgg agacaggccc ttgttccgcg ccaagcgaac cggatcaagc cagaacttac
     3901 actcaccttc acttgactca acatgaatat gcggagcctc acctccctca ttggaataga
     3961 aatggaagcg aaacggtccg atacgcaaga cggttggcat tatgcaaccc ctgaataaat
     4021 atatatcaag aacattatgc cgtcaaatag aatcatacgg aggcatgcat cagcacccgc
     4081 tcgtacaccc cgaccatcct gcgcaccgcg gcctcccacg agaagtgctc cgcgacgcgc
     4141 gcttggcccg ccgcgcccag gctcttcatc ttttttgggt cggccaggag cgcatggatc
     4201 gcctcggcga gggcgtagct gtcgcgtgcg ggaacgatga cgccggcgtc gcccaccacc
     4261 tcggggagcg ccccgccgtc gctggcgatc acccgcggcc gaggccatgg cctgcggcac
     4321 gccgggaacc cgaagccctc g
//
LOCUS       DAORHF010000293         4337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21689_length_4337_cov_3.957730, whole genome shotgun sequence.
ACCESSION   DAORHF010000293 DAORHF010000000
VERSION     DAORHF010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21689_length_4337_cov_3.957730"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..505
                     /locus_tag="PKJ16_16955"
     CDS             68..505
                     /locus_tag="PKJ16_16955"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48734.1"
                     /translation="MAGVHLAHNTKVGNEVIIANNTVLAGYVEVDDFCFVSGLVGFHQ
                     FVKIGKYCMISGMSRINMDCVPYMIHEGNPSRIAGINIVGLRRRGFSEERRRTIKNLY
                     KLLVRSKLNTTQAVERIKAEYGNDPDGQEMLAFIERSDRGINK"
     gene            692..1417
                     /locus_tag="PKJ16_16960"
     CDS             692..1417
                     /locus_tag="PKJ16_16960"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HNV48735.1"
                     /translation="MNKQSHENVLKPRLNVARSLVLALGLMVAFSTSCVADVKSELRK
                     AFAKHPGPYAIHVSKGEFTLRVINRKLETVASYRVGYGRNGDRRPKLYEGDLRTPEGT
                     YEVTEILSMDAGKNTESYRKLKMMNETHFYAKDGHFKFGRPKEDLGTDVYGPRFFRIS
                     YPNAADERRYAAALKKGSIPRSAEDPDEPAGIGFGIAIHGNNDAPGVGQLSSSGCVRM
                     RNNDVVELDRFVRLGTPVVITAN"
     gene            1540..2811
                     /locus_tag="PKJ16_16965"
     CDS             1540..2811
                     /locus_tag="PKJ16_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48736.1"
                     /translation="MIKKITITVLAASLCMLSVRCIKSDGELGDSTVVINLGLDQAFN
                     APKTRFDRFMNFIMPKAYAAPSNIASVSIVVSAEDIKTFENSYSPPPTYITVTVPAGK
                     SRFIQVIGYVKDGDPSAVLSYRGTLTADLEPGETKEVAMQMVTYETKLVIPDFRNYRI
                     VQIENMAGKNWIDRNGSQIGYGGAMQPYDIDFDAQGRIYIISNSTTPLMVRIDNIYST
                     TCDTIVTAGLTNPSIAIDRKSNLVYYTQNANDPSTVYRRDTAGGNLQSFTLNGVEFIR
                     GLAVDTDGILYIATTQKIHKFNPSGSGTLIGSYTFQINGARDICVKDDKVFVSNNAGA
                     NGYRFIELDKNLRFVRGYGSVLPGGSPSGPGKFYGPERIVAKTNRKIFVIDESDPGYD
                     QLLAFDDIDGENWQSYGSKGVGVGYFKFYEC"
     gene            2815..3693
                     /locus_tag="PKJ16_16970"
     CDS             2815..3693
                     /locus_tag="PKJ16_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48737.1"
                     /translation="MRIIYYTSGITGSGRIVRGIAIGNALRRKGLDAEYAILSSSDFA
                     FLCDELKFAHREIPLEDEEALSDRNYKSSHLFNELSRLRPDVLIVDLLWFPLYHFIGD
                     LACKKIFLCRQVNDRFFQMELNGGLLDFNPDAFDFVLRIEPFDSKVRMKAISPIIVRN
                     RDELLSREQACARIGLSHNEQNCLIAYNGEPGEYHQVVKEYSYLQEQGYVLKFSSNYE
                     NGLFPIADYYNAFDLVVCGAGYNFFWEMIYFNKEAIFVPAVRTFETGQRRMREYRDFD
                     FSQNGADQLVDEYLLS"
     gene            complement(3744..>4337)
                     /locus_tag="PKJ16_16975"
     CDS             complement(3744..>4337)
                     /locus_tag="PKJ16_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HNV48738.1"
                     /translation="NDNKAEIVVVANNYWNSSVNQGVYVFGDANNTWVNTRKIWNQHS
                     YHVTNVNDDATIPRVEQNNWITYNNYRQNQLERPSGCFDVSASYVRVTPDANPGDYTI
                     TARIGNSGALAIGNTIDVALFDGNPETGGTLLGVSQITNRMEVGACVDVTFTWSGPAA
                     GTHSIYIVADSNGTGLSRLREMNEGNNTGTTSINITP"
BASE COUNT         1014 a         1046 c         1205 g         1072 t
ORIGIN      
        1 ggttgaccac gcccagcgag cgtgaagcca tcgcgtagcc gtcggccatc gccaccgcgc
       61 aatgctcatg gcgggcgtgc acctggcgca caacacgaag gtcggcaacg aggtgatcat
      121 cgcgaacaac acggtgcttg ccggctacgt ggaggtggac gacttctgct tcgtctcggg
      181 actggtgggc ttccatcagt tcgtgaagat cgggaagtac tgcatgatca gcgggatgtc
      241 gcgcatcaac atggactgcg tgccgtatat gatccacgag ggcaacccct cgcgcatcgc
      301 gggcatcaac atcgtgggcc tgcgccgccg cgggttctcc gaggagcgcc gccgcaccat
      361 caagaacctc tacaagctgc tggtgcgttc caagctcaac accacgcagg cggtcgagcg
      421 catcaaggcc gaatacggca acgatcccga cggacaggag atgctcgcgt ttatcgagcg
      481 atcggaccgg gggatcaata agtaaccgcc ccctcccggg ccgagcgtta ttagacgtcc
      541 tgtccacgct cggcactgca atatcctgtt gggagcctcc ctcttcgtta ggggaggggt
      601 ggcgagaggt ttcttgttgg catctccccc tagcgaaggg ggagatggcc ggaggccaga
      661 gggggtaaat gccgcactgg atggaatcga aatgaacaaa caatcgcatg agaacgtact
      721 gaagcctcgg ttgaatgtcg cgagatcgct cgtcctggcg ctcggtctca tggtcgcgtt
      781 ctcgacgtcg tgcgttgcgg acgttaagag cgagctgcgg aaggcctttg cgaagcaccc
      841 ggggccgtat gccatccatg tgagcaaggg cgagttcacg ctgcgcgtca tcaatcgaaa
      901 gctcgagacg gtcgccagct accgcgtcgg gtacggccgg aacggcgatc gccggccgaa
      961 gctctacgag ggcgacctgc gcacccccga ggggacctac gaggtgaccg agatcctgag
     1021 catggacgcc gggaaaaaca ccgaatcgta ccgcaaactc aagatgatga acgagaccca
     1081 tttttacgcc aaggacggcc acttcaagtt cggccgtccg aaggaggacc tggggaccga
     1141 cgtatacggc ccgcgcttct ttcgcatcag ctatccgaac gccgccgacg agcgcaggta
     1201 cgccgctgcg ctcaagaaag gctccatccc gcgctcggcg gaggacccgg acgagccggc
     1261 ggggatcggc ttcgggatcg ccatccacgg caacaacgac gcgcccggcg tgggccagct
     1321 ctcctcgagc ggctgcgtgc gtatgcgcaa caacgacgtg gtggagctgg accgcttcgt
     1381 tcgcctgggg acgccggtcg tcatcaccgc gaactagata tttttgttga catttccgcg
     1441 gaaaatcgta cttttataaa cgtaccgaat ataaagagac cgtccaactc gctcccagtg
     1501 tgttttttgc ggaagagtat gtgcgtgagg agtttctgta tgataaagaa aatcacaatt
     1561 accgttctgg cggcgtcatt gtgcatgcta tccgttcgct gcatcaaatc cgacggagag
     1621 cttggcgatt ccaccgtggt gatcaacctc ggcctcgacc aggccttcaa tgctccaaag
     1681 acccggttcg accggttcat gaatttcatc atgccgaagg cgtatgcggc gccgtcgaac
     1741 atcgcgagcg tgtccatcgt ggtgagcgcc gaggacatca agaccttcga gaattcctac
     1801 tcgccgccgc ccacctatat caccgtgact gtccccgcgg gcaagtcgcg ttttatacag
     1861 gtgatcggct acgtgaagga cggcgatccg agcgcggtgc tctcctatcg cgggacgctc
     1921 accgcggatc tcgagccggg ggagacgaaa gaagtcgcca tgcagatggt gacatatgaa
     1981 acgaagttgg tgattccgga ttttagaaat tatcgtatcg ttcaaataga aaacatggct
     2041 gggaagaatt ggattgatcg aaatggttcg cagatcggtt atgggggagc aatgcagcca
     2101 tatgatattg actttgatgc ccaagggagg atttatataa tttctaattc aacgacccca
     2161 cttatggtga ggattgataa tatttatagt accacgtgcg atacgattgt tactgctggg
     2221 cttacgaatc cttcgatcgc gattgaccga aaaagtaacc ttgtttatta tactcaaaat
     2281 gctaacgatc ctagtactgt atatagaaga gatacggctg ggggtaatct ccagtccttt
     2341 acactcaacg gcgtggagtt tatccgtggc cttgcggttg acaccgatgg aattttatat
     2401 atagcaacta cgcagaagat tcataaattt aatccaagtg gaagtgggac gctgattggg
     2461 tcgtatactt ttcaaatcaa cggggctagg gatatttgtg ttaaggatga caaggtgttt
     2521 gtttcaaata atgctggagc gaatggctat agatttattg agcttgataa gaatctaagg
     2581 tttgtacggg ggtatggttc agttttaccg gggggttctc catctgggcc tggcaaattt
     2641 tatggtccgg aacgtattgt tgcaaaaaca aatagaaaga tttttgtgat tgatgaaagt
     2701 gatcctgggt atgatcagtt acttgccttt gatgatattg atggtgaaaa ttggcaatca
     2761 tatggctcga aaggtgtggg tgtcgggtac tttaaattct acgagtgttg atccttgcgg
     2821 ataatttatt atacctcagg aataactggt tccggtagaa tcgtaagggg cattgcaatc
     2881 ggcaatgccc ttcgtcgtaa ggggcttgat gcagagtatg ccatcttgag tagttccgat
     2941 ttcgcgtttc tttgcgacga attaaaattt gcacatagag aaataccatt agaggacgag
     3001 gaggcactct cagacagaaa ttataagtct tcccatttgt ttaatgagtt gtcgcgcttg
     3061 cgtccggatg tcctcattgt tgatctcctc tggtttccgt tgtaccactt cattggtgat
     3121 ctcgcatgta aaaaaatatt tctctgtcgt caggtgaacg atcgattttt ccaaatggaa
     3181 ctgaatggtg gtttgttaga ttttaatcct gacgccttcg attttgtctt gagaattgaa
     3241 ccgtttgact caaaagtgcg aatgaaggcc attagcccaa tcattgtacg caatcgggat
     3301 gaattgcttt cgagagagca ggcttgtgca cgcatcggct tgtcgcataa cgaacagaat
     3361 tgtttaatcg cctacaatgg ggaacctggc gagtatcatc aagtggtaaa agaatattcg
     3421 tatttgcaag agcagggcta tgtgttaaaa ttttcatcga attacgagaa cggcttgttc
     3481 ccaattgcgg attactacaa tgctttcgat ctcgttgtgt gcggggccgg gtataatttt
     3541 ttctgggaaa tgatttactt taacaaagaa gcgatatttg ttcccgcggt acgtacattc
     3601 gagacggggc agcgccgaat gcgggagtat cgtgattttg atttctcgca aaacggcgct
     3661 gatcagctgg tggatgaata ccttctttcg tgaatgtgaa aaaaaacatt aatgaacgaa
     3721 ataaaaaatt gcacaatatt tcgctaggga gtaatattta ttgacgttgt ccccgtatta
     3781 ttcccctcat tcatctcccg caaccgactc aatcctgtcc cattactgtc agcgacgata
     3841 tagatcgaat gtgtcccggc cgccggtccg ctccacgtga atgtgacgtc cacgcatgcg
     3901 ccaacctcca tgcggttggt tatctgactc acgccaagca gcgttccgcc ggtctccggg
     3961 ttgccgtcga agagtgctac atcgatagtg tttccaattg cgagcgcgcc gctgttgccg
     4021 atgcgggcgg tgatggtata atcgcctgga tttgcatcag gcgtgacgcg gacgtacgac
     4081 gcggacacgt caaagcatcc ggatggtcgc tcaagctggt tctggcggta gttgttgtac
     4141 gtaatccagt tgttctgttc tacgcgcggg atggtggcat cgtcgttcac gttggttacg
     4201 tgatacgagt gctggttcca gatcttgcgg gtgtttaccc aggtgttgtt cgcatcgccg
     4261 aagacgtaaa ctccttgatt gaccgatgaa ttccagtaat tattcgcgac aacaacaatt
     4321 tcggccttgt tgtcgtt
//
LOCUS       DAORHF010000294         4334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21716_length_4334_cov_4.351951, whole genome shotgun sequence.
ACCESSION   DAORHF010000294 DAORHF010000000
VERSION     DAORHF010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21716_length_4334_cov_4.351951"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="tsaB"
                     /locus_tag="PKJ16_16980"
     CDS             <1..547
                     /gene="tsaB"
                     /locus_tag="PKJ16_16980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HNV48739.1"
                     /translation="PGDLELLGVGVGPGSFTGVRIAVATARMLAQALSLPLIGVPSPL
                     IHAAAIDAASGSNILIAFDAKKERVFGALYVVAGDGSLDEIVAPGDYPIGTLIEGVDR
                     AAPVHLAGDGIGPYWDRGLSSLANAHHHERFVPSGVRACELVRGIYARANGGPFPYER
                     VVPHYARKSDAEILRDATQRG"
     gene            complement(544..1731)
                     /locus_tag="PKJ16_16985"
     CDS             complement(544..1731)
                     /locus_tag="PKJ16_16985"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HNV48740.1"
                     /translation="MLNVKQRKQRTFDDLASVDDVIELLKTEFRTRPLRVKYSLEGSE
                     VQINEYLDDKSLMMITDPDFRPDDDTIVIYGLLDKYIEIDLQVVETRGPGYFHCRIRS
                     VRKAKTGRRDLRFKSGPGEVVTTNFRVSKHTIDVSGLIVPTGIKVILDQFQTQNSKLS
                     DIVKVGVFDTEDVVLKLIKKTGKTFYVEDAANPESYRAVTEDFIDLKEFLGDDFSRYI
                     KKVVERGYKSIIACPLIYITESEQSFPFAYIQLISKSENFSMDKLLDVKGMTFKLVER
                     IRDANTLTIPVHQQVVDISRGGAKIMITDDNLKKYLIRARGFIFDIVFKLQAPITIYG
                     EIRYTNVDDDGNMFIGVDFAGNSSRKGEMKRFYSIIKPMELEYKSRLLKDLKKRKKAD
                     GEA"
     gene            complement(1933..2946)
                     /locus_tag="PKJ16_16990"
     CDS             complement(1933..2946)
                     /locus_tag="PKJ16_16990"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HNV48741.1"
                     /translation="MKNVNVVILGATGAVGVEFRKILLERKFPIGKIKFLGNSTVGQT
                     IEFGGRSVTVEAAREDSFAGYQLGLFSAGATISKQVARAAAAAGCVVVDNSSAWRMEK
                     DIPLVVPEVNPNDVDWHPGIIANPNCSTIQMVVALKPLHDAARIKRIVVSTYQAVSGT
                     GLKAVDELTIQTRDIMAGKPISQRKVYPHQIAFNALPQIDVFLENGYTKEEMKMVNET
                     KKIMGDDAIQVTATTVRVPVFYGHSESVNIETEKKLSAQAVRELLAGAPGVTVVDDPA
                     NGRYPLALDAAGKDDVFVGRIREDESIPNGINMWIVSDNIRKGAALNAVQIAELLMKK
                     NII"
     gene            complement(3037..3402)
                     /locus_tag="PKJ16_16995"
     CDS             complement(3037..3402)
                     /locus_tag="PKJ16_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48742.1"
                     /translation="MEKRIILHSVDGDRSCSLPAGEWERLIGLARERGWSPEGTRLDY
                     RFECFMKIDDDDEPDDAFFEAICIQQYCLGWDGNYLDPMNQVVSADDVYDLLPALMDA
                     GAPPDLLCVLRDGAFRICA"
     gene            complement(3495..4025)
                     /locus_tag="PKJ16_17000"
     CDS             complement(3495..4025)
                     /locus_tag="PKJ16_17000"
                     /inference="COORDINATES: protein motif:HMM:NF033223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH domain-containing protein"
                     /protein_id="HNV48743.1"
                     /translation="MKRIVALSVVCMFALVQMVFAHGGRTDKDGGHCDYSKGEYHYHK
                     DGKVTVDSAKSAQCQKKGKRDDSKSSKGKKVEKGDDAGKDAVADKAKDKKKKGKDKDA
                     DTDKKKDDKKSDKDKKKDDKKAEKDKKKEEKKLAKEKAKKCKADNAACKKACKKGDKK
                     CTSSCKDEYEKCKKAA"
     gene            complement(4100..>4334)
                     /locus_tag="PKJ16_17005"
     CDS             complement(4100..>4334)
                     /locus_tag="PKJ16_17005"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV48744.1"
                     /translation="IVRAENGEDVLEIKIEISEGIFNDELKKMLELKNKIARSFEADL
                     GIAPHITLVEQDSLRSAGGGKVNRIVDMRSGRV"
BASE COUNT          760 a         1318 c         1229 g         1027 t
ORIGIN      
        1 gcccggggac ctggaacttc tgggcgtcgg cgtgggcccg gggtccttca cgggtgtccg
       61 catcgccgtc gccacggcgc ggatgctggc gcaggcgctc tcgctgccgc ttattggggt
      121 gccctccccg ctcatccatg cggccgcgat cgacgcagcc tcgggatcga acatactgat
      181 cgccttcgac gccaagaagg agcgtgtctt tggcgccctg tacgtagttg ccggcgacgg
      241 ttcgctcgat gagatcgtcg caccggggga ttatcccatc ggaaccctta tcgagggcgt
      301 cgatcgcgcc gccccggtcc acctcgcggg cgacggaatc gggccatact gggaccgggg
      361 gctttcttcg ctcgccaatg cgcatcatca tgaacgcttc gttccgtcgg gggtgcgcgc
      421 atgcgaactg gtgcgcggca tctatgcacg cgcgaacggc ggaccgttcc cctacgaacg
      481 ggttgtgccg cactatgcgc ggaaatcgga cgccgagatt ctcagggatg caacacagcg
      541 gggctaggct tcgccgtcgg ccttttttcg ctttttcagg tctttcagca ggcggctttt
      601 gtattccagc tccatgggct tgatgatcga gtaaaaccgc ttcatctcgc ccttgcgcga
      661 ggagttcccc gcgaaatcga cgccgatgaa catgttgccg tcgtcgtcca cgttggtgta
      721 gcggatctcg ccgtagatgg tgatgggcgc ctggagcttg aagacgatgt cgaagatgaa
      781 accgcgggcg cggatcaggt acttcttgag attgtcgtcg gtgatcatga tcttcgcccc
      841 gccccggctg atatccacca cctgctggtg caccgggatc gtgagcgtgt tcgcgtcgcg
      901 gatgcgctcg acgagcttga aggtcatccc cttgacgtcg aggagcttgt ccatgctgaa
      961 attttcgctc ttcgagatga gctggatata ggcgaagggg aacgactgtt ccgattcggt
     1021 tatgtagatg agcgggcagg cgatgatcga cttgtacccc cgctcgacca ccttcttgat
     1081 gtaacgggag aaatcatcgc ctaaaaactc cttaaggtcg atgaaatcct cggtcacggc
     1141 ccgataggac tcgggattcg cggcgtcctc cacgtagaag gtcttgccgg tcttcttgat
     1201 gagcttgagg accacgtcct cggtgtcgaa tacgccgacc ttcacgatgt ccgaaagctt
     1261 cgagttctgc gtttggaatt ggtccaggat caccttgatc ccggtgggca cgatgaggcc
     1321 ggaaacgtcg atcgtgtgtt tcgagacccg gaagttggtg gtcacgacct cgccggggcc
     1381 gcttttgaag cggaggtccc gccggccggt tttcgccttg cgcaccgacc tgatccggca
     1441 gtgaaaatac ccgggaccgc gcgtttcaac cacctggagg tcaatttcaa tatacttgtc
     1501 gaggaggccg tagatcacga tggtgtcgtc gtccggccgg aagtccgggt ccgtgatcat
     1561 catcaggctc ttgtcgtcga ggtattcgtt gatctgcacc tcgctcccct cgagggaata
     1621 cttcacccgc aggggccgcg tccgaaactc ggtcttcaac agctcaatga cgtcgtcaac
     1681 cgacgcgaga tcatcgaagg ttcgctgctt tctctgctta acatttagca cggaaacggc
     1741 tccacggtgc gatttgaact ctgtacgccg gtatgatccg ctttgtttga gtctgcgcgt
     1801 atatatctat tatcgaaaca ataactgagt atttttatac attatttatc attcatgccc
     1861 gcacgatgca ataaaaaaaa ccccctgccg cgcctgggcg gagggggcct tttttcgaga
     1921 aaccgccgca ggctaaatga tgtttttctt catcaaaagc tcggcgatct gaacggcgtt
     1981 gagcgccgcg cccttgcgga tattgtccga cacgatccac atgttgattc cgttcggtat
     2041 cgactcgtcc tcccggatgc gaccgacgaa cacgtcgtcc ttgcccgcag cgtcaagcgc
     2101 caacgggtag cggccgttcg cgggatcgtc cacgacggtc acgccggggg cccccgcaag
     2161 gagctcccgg acggcctgcg ccgacagctt cttctcggtt tcgatgttca ccgattccga
     2221 gtgcccgtaa aacaccggga cgcgaacggt ggtggccgtc acctgaatgg catcgtcccc
     2281 catgatcttc ttggtttcat tgaccatctt catctcttct ttggtgtacc cgttctccag
     2341 gaacacgtcg atctggggaa gcgcattgaa ggcgatctgg tgcggataga ccttccgctg
     2401 cgagatgggc ttcccggcca tgatgtcccg ggtctgaatg gtgagctcat cgaccgcctt
     2461 gagacccgtg ccggagaccg cctggtaggt gctcacgacg attcgcttga tgcgcgccgc
     2521 gtcgtgcagc ggcttgagcg ccaccaccat ttgtatggtc gagcagttcg ggttggcgat
     2581 gatcccggga tgccagtcca catcattcgg attgacctcg ggaacgacaa gcgggatgtc
     2641 cttttccatg cgccaggccg agctgttgtc gaccacgacg cacccggcgg cggccgccgc
     2701 gcgcgccacc tgcttgctga tcgtagcccc ggccgagaac aggccgagct ggtatcccgc
     2761 gaaagaatcc tcgcgggccg cctcaaccgt cacggaccgg cccccgaatt cgatcgtctg
     2821 gcccaccgtg ctgttaccca ggaactttat cttcccgatc gggaatttgc gctcgaggag
     2881 tattttgcgg aattcgacgc cgacggcacc ggtagccccg agaatcacga cattgacgtt
     2941 tttcataaca cctcctcgca agcgcatgcg cacgctcatt gtggttgggc aatagttgcg
     3001 gcggagtgtt tatttgcaac caaatatttc aggggatcat gcgcagatcc ggaaggcgcc
     3061 gtcgcgcaac acgcacaaca gatccggtgg cgcgccggca tccatgagcg ccggcaggag
     3121 gtcgtacaca tcatcggcgg agacgacctg gttcatgggg tccagatagt tcccgtccca
     3181 gcccaggcag tattgctgga tgcagatggc ctcgaaaaag gcgtcatcgg gctcgtcgtc
     3241 atcgtcgatc ttcatgaagc attcgaagcg gtaatcgagc cgggtgccct ccggcgacca
     3301 tccgcgctcc cgggcgaggc cgatcaggcg ctcccactcg cccgcgggca gggaacagga
     3361 gcgatcgccg tctacactat ggaggatgat gcgtttctcc atctcacccc gtcaccaggg
     3421 tccgaaaaca cgcgagggca aggatacact ccccgccctc gctgcgttca gccaatcgat
     3481 gccgcgacgt ggaactatgc ggcctttttg cacttttcgt attcgtcctt gcacgaggag
     3541 gtgcacttct tgtcgccctt cttgcaggct ttcttgcagg cggcattatc ggccttgcac
     3601 ttcttggcct tttccttggc gagcttcttt tcttctttct tcttgtcctt ttcggccttt
     3661 ttgtcgtctt tcttcttgtc tttgtcggat tttttgtcgt ccttcttctt gtccgtatcg
     3721 gcgtccttgt ctttgccctt tttcttcttg tccttcgctt tgtccgctac ggcgtccttg
     3781 ccggcgtcat cgcccttctc gaccttcttg cccttcgagc tcttggaatc gtcccgcttg
     3841 cctttctttt ggcactgggc ggacttggcg gagtcgacgg tcaccttgcc gtccttgtgg
     3901 taatgatatt cgcccttcga gtaatcgcaa tggccgccgt ccttgtccgt ccttccgccg
     3961 tgcgcgaaca ccatctgcac cagcgcgaac atgcatacaa ccgataatgc aacaatgcgt
     4021 ttcatatgac agatctccta tggaatgatt ttgcgtatca tagggacccg cacgggcccc
     4081 gtcaacacaa atattgcgac tacacacgcc ccgagcgcat gtccacgatg cggttcacct
     4141 tcccgccgcc cgcactccgg agactgtcct gctccaccag ggtgatgtgg ggagcgatcc
     4201 ccagatcggc ctcgaacgac cgcgcgattt tattcttgag ctccagcatc ttcttgagct
     4261 cgtcgttgaa tatgccctcg gatatctcga ttttgatctc gaggacgtcc tcgccgttct
     4321 cagcgcgcac gatg
//
LOCUS       DAORHF010000295         4321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21819_length_4321_cov_4.877403, whole genome shotgun sequence.
ACCESSION   DAORHF010000295 DAORHF010000000
VERSION     DAORHF010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21819_length_4321_cov_4.877403"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PKJ16_17010"
     CDS             <1..395
                     /locus_tag="PKJ16_17010"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013970062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HNV48745.1"
                     /translation="GGPGMQEMLYPTSYIKSMGLGKECALITDGRFSGGTAGLSIGHI
                     SPEAAAGGEIALVEDGDVIEIDIPARSINVRIESAELEARRAKERAKGNRAFTPAGRD
                     RTVSQSLKVYAMLAASADRGAVRVLPEE"
     gene            complement(402..1208)
                     /locus_tag="PKJ16_17015"
     CDS             complement(402..1208)
                     /locus_tag="PKJ16_17015"
                     /inference="COORDINATES: protein motif:HMM:NF021613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HNV48746.1"
                     /translation="MGIDDIKYLVFDIESIPDARLIRMVKYPHLDIDEKAAVLKFQEE
                     VLDNSGGTTTFIPATFQYPVSICILKCREDFTIADIVSLDEPEFRPGEMTRLFWQGVE
                     KQYDKASLVTFNGRGFDIPLMEFMAFRYGITAQRHFRDKFAGRYRFGTKHIDLHDWLS
                     NYNAVRVAGGLNLLAKLLGKPGKMDAKGDEVYDMYLAGKKKEINDYCIHDVLDTYFVF
                     LRTRVMQSELTIEREQDLVKMAKNYIEENQQRIPALQIYLKNWGDWDPWP"
     gene            complement(1288..1770)
                     /locus_tag="PKJ16_17020"
     CDS             complement(1288..1770)
                     /locus_tag="PKJ16_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HNV48747.1"
                     /translation="MKKFLISFVSVFMLISFLNAQDAAKKEEKPKTTKEYINDLGSTD
                     ENAIVKACDYLGQKEEKDAIPKLLPLLKDSGKSAQVRMWSAIALGLIGDEKTLDPLAE
                     SVQKDSSADVRYSAILAMSRIGVKDKKYLELFKDRMNGDPDPFIRDYLTKLYEKAGGK
                     "
     gene            complement(1905..>4321)
                     /locus_tag="PKJ16_17025"
     CDS             complement(1905..>4321)
                     /locus_tag="PKJ16_17025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HNV48748.1"
                     /translation="AGGELNAIVRERAAVRMAICYPDLYEIGMSNNGIQILYHAANRM
                     EDVACERVFTVEADLEARLRSLDLPLFTLETRTPLSELDLLGFSVAHELLATTVLQVL
                     DLGGVPLLRSERGGRDPIVIAGGAGCSNPLPLSDFIDAFFIGDGEEGIIDILSVLKAM
                     RAEGRSRADIIAGLSSIEGVYVPSVHDGTGRTIRKRVYRAAHPLVPERPVVPSIRITQ
                     ERVALEVTRGCGNLCKYCHAGYYELPYRTYPPDRFRNDIITLLDNTGYEELSLSSLSL
                     GDYRHLAALLNDIMPALTERGVSLSLPSLRVDRATLGIIERLSDIRRSTLTFAVEAAA
                     EDMRLRANKRISTDELVEIIDHVFARGWRAVKLYFMLGMPGCEEHDEAADIAALLKRL
                     LPPGGKKREINVTLSPFIPKPHTPFQRERQMGMDYFDDAVRRIKQSVPRRIAIKNHDI
                     RASTLEGALSRGDARLGRAILAAYRAGCRLDSWKEHFRFDLWRQALDEHAPGWEAYLG
                     PRDETRPLPWGFIATGFERLTDHMTRRVATVAHPRPDAGDVPALDTAAIEQAFARFTR
                     RYATVSRMRLRIEKIGDARLVPHIDFMEIVKRALRMASAPVSFTQGFNKRERISAGFP
                     LPLGVQSVAELIDVDLFEQADPAALAAALNRALPRGISIATAREIHGAESIMAITSAA
                     RFIAGIADSAIGDRLTASLAAGRSLVKNGKNGPREVACADAIIGHGAIAGADCGDDAD
                     TAIRLLRGSPAIITLTLAVGTEHSIRIDDLLRQLSGAAPEDAHLFTMVKTAQLRGAAG
                     TYSEIT"
BASE COUNT          728 a         1319 c         1467 g          807 t
ORIGIN      
        1 agggcggccc cggcatgcag gagatgctct atcccacctc gtatatcaag tcgatgggtc
       61 tcgggaagga atgcgcgctc atcaccgacg ggcgcttctc cggggggacg gccggcttgt
      121 ccatcggcca catatcgccg gaggcggcgg ccggcggcga gatcgcgctc gtcgaggacg
      181 gcgacgtcat cgagatcgac attcccgcgc ggtccatcaa tgtccgcatc gagagcgcgg
      241 aactggaggc ccgccgtgcg aaagaacgcg ccaaggggaa ccgcgccttc accccggccg
      301 gacgggatcg gaccgtatcg cagtcgctca aggtgtacgc gatgctcgcg gcctcggccg
      361 accggggcgc ggtgcgcgtg ttgccggagg agtagccctt cctacggcca cgggtcccag
      421 tcgccccagt tcttaaggta gatctgcagg gcggggatcc gctgctgatt ctcctcgatg
      481 tagtttttcg ccatcttgac gaggtcctgc tcgcgctcga tggtgagctc gctttgcatc
      541 acccgcgtgc gtaggaagac gaagtacgtg tccagcacgt cgtggatgca gtagtcgttg
      601 atctccttct tcttgccggc gaggtacatg tcgtacacct cgtcgccctt ggcgtccatc
      661 ttgccgggct tgcccaggag cttcgcgagc aggttcaacc ctcccgccac gcgcacggcg
      721 ttgtaattcg agagccagtc gtgcaggtcg atgtgcttgg tgccgaagcg gtagcgaccg
      781 gcgaacttgt cgcggaagtg ccgctgcgcg gtgatgccgt agcggaaggc catgaactcc
      841 atcaggggga tgtcgaagcc gcgtccgttg aaggtcacca gcgaggcctt gtcgtactgc
      901 ttctcgacgc cctgccagaa gaggcgggtc atctcgcccg ggcggaactc gggctcgtcc
      961 aacgagacga tgtcggcgat ggtgaaatcc tcgcggcact tgaggatgca tatggacacc
     1021 gggtactgga aggtcgccgg gatgaacgtg gtggtgcccc cggaattgtc cagcacctcc
     1081 tcctggaact tcaagacggc ggccttctcg tcaatatcga ggtgcggata ctttaccatg
     1141 cggatcagcc gcgcgtcggg gatcgactcg atgtcgaaaa ccagatattt gatgtcgtcg
     1201 atgcccacag gacctccgga ataaaaaaag catgttttag tcgctaaaac atgctttgtg
     1261 tgatggtgcc gaattaagcg gcggggctta ctttccgccc gccttctcgt acagcttggt
     1321 gagatagtcc cgaatgaagg gatcggggtc gccgttcatg cggtccttga acagctcgag
     1381 atacttcttg tccttcacgc cgatgcggct catcgcgagg atggcgctgt agcggacgtc
     1441 ggcgctggag tccttctgga cgctctccgc aagcggatcc agggtttttt cgtcgccaat
     1501 gaggcctagg gcgatcgcgg accacatgcg cacctgcgcc gattttccgc tgtccttcag
     1561 gagcgggagc agcttgggaa tggcgtcctt ttcttccttt tggccgagat agtcgcaggc
     1621 cttcacgatg gcgttctcgt cggtgctgcc gagatcgttg atgtattcct tggtcgtttt
     1681 cggtttctct tcctttttgg cggcgtcctg cgcgttcagg aagctgatga gcatgaatac
     1741 ggacacgaac gagatcagga atttcttcat ggcatacctc aatggaagaa tgggtgtaat
     1801 cgtcatatgc ggaatatacg ccgaacgacg atgaggagag tatccgtccg ttgattttat
     1861 gtcaatactt ttattttttt catttttgtc gctctgatat cgcatcatgt gatctcggaa
     1921 tacgttcccg ccgcgccacg gagctgggcg gttttcacca tcgtaaagag atgcgcgtcc
     1981 tccggagcgg cgccggagag ctggcgcagc aggtcgtcaa tccggatcga gtgctcggtc
     2041 ccaacggcca gggtcagggt gatgatggcg gggctccccc gaaggagccg tatggccgta
     2101 tcggcgtcgt cgccgcagtc agcgcccgcg atcgcgccat gcccgatgat ggcgtccgca
     2161 caggcgacct cgcgggggcc gttcttcccg ttttttacca gcgagcggcc cgcggccagt
     2221 gaggcggtca gccggtcgcc gatggcgcta tcggcgatcc cggcgatgaa ccgcgcggcg
     2281 gacgtgatcg ccatgatgga ttccgcgcca tggatctcgc gcgcggtcgc gatggatatg
     2341 ccgcggggaa gggcgcggtt gagcgccgcg gcgagcgccg cgggatcagc ctgctcgaag
     2401 aggtccacat cgatgagttc ggccacgctc tggacgccga ggggaagcgg gaagcccgcc
     2461 gagatccgct cgcgcttgtt gaacccctgc gtgaaggaca ccggtgccga cgccatgcgg
     2521 agcgcgcgct tcacgatctc catgaaatcg atgtgcggga ccaggcgcgc atctccgatc
     2581 ttttcgatcc gcagtcgcat gcgcgacacc gtcgcgtacc ggcgcgtaaa gcgcgcgaag
     2641 gcctgctcga tggccgccgt gtccagcgcg ggaacgtccc cggcatccgg gcgcgggtgc
     2701 gcgacggtcg cgactctccg cgtcatgtga tcggtgaggc gctcgaagcc ggtcgcgatg
     2761 aatccccacg gaagcgggcg cgtttcgtcg cgcggcccga gataggcctc ccagcccggc
     2821 gcgtgctcgt cgagggcctg ccgccacagg tcgaaacgga agtgctcctt ccacgagtcg
     2881 agccggcagc ccgcccggta ggccgcgagg atcgcgcgcc ccagccgcgc atcgccccgc
     2941 gagagcgcgc cctcgagcgt ggaggcgcgg atgtcgtggt tcttgatcgc gatacgtcgc
     3001 gggaccgact gtttgatgcg ccggacggcg tcatcgaaat agtccatgcc catctgccgt
     3061 tcgcgctgga agggcgtgtg gggcttgggg ataaacggcg agagcgtcac gttgatctcg
     3121 cgcttcttgc cgccgggggg caggaggcgc ttcaggagcg cggcgatgtc ggccgcctcg
     3181 tcgtgctcct cgcaccccgg catgcccagc atgaaataca gcttcacggc gcgccatccg
     3241 cgcgcaaaga cgtggtcgat gatctcgacg agctcgtcgg tggatatccg cttgttcgcg
     3301 cgcagccgca tgtcctcggc ggccgcctcg acggcgaagg tgagcgtgct gcggcggatg
     3361 tcgctcaggc gctcgatgat gcccagcgtg gcgcggtcca ccctgagcga cggcagggag
     3421 agcgaaacac cgcgctccgt cagcgcgggc atgatgtcgt tcaggagcgc ggcgaggtgc
     3481 cggtagtccc ccagcgagag cgaggaaagc gaaagctcct cgtagccggt gttgtcaagc
     3541 agggtgatga tgtcgttccg aaatcgatcg ggcgggtagg tgcgatacgg cagctcatag
     3601 tagccggcgt ggcagtactt gcacaggttg ccgcagccgc gcgtgacctc gagggcgacg
     3661 cgctcctgcg tgatgcggat gctggggacc acgggccgct cggggaccag cgggtgcgcg
     3721 gcgcgataga cccgcttgcg gatggtgcgc cccgtgccgt cgtggacgga agggacgtac
     3781 acgccctcga tggaagaaag ccccgcgatg atgtcggcgc gggaccgccc ctccgcgcgc
     3841 atcgccttca gcactgaaag gatatcgatg atcccctcct cgccgtcgcc gatgaaaaag
     3901 gcgtcgatga agtcggacag ggggagcggg ttggagcacc ccgcgccgcc cgcgatcacg
     3961 atggggtcgc gcccgccgcg ctcgcttcga agcagcggaa cgccgcccag gtcgaggacc
     4021 tgcaggacgg tcgtcgccag gagctcgtgc gcgacgctga agccgaggag gtcgagttcg
     4081 gaaagcggcg tgcgcgtctc gagcgtgaac agcggcaggt cgagcgagcg caagcgcgcc
     4141 tcgagatcgg cctccaccgt gaagacgcgc tcgcaggcga cgtcctccat ccggttggcg
     4201 gcgtgataca ggatctggat gccattgttc gacatcccga tctcgtagag gtccgggtag
     4261 cagatcgcca tgcgcacggc ggcgcgctcc cgcacgatcg cgttgagctc cccgccggca
     4321 t
//
LOCUS       DAORHF010000296         4307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_21926_length_4307_cov_4.116416, whole genome shotgun sequence.
ACCESSION   DAORHF010000296 DAORHF010000000
VERSION     DAORHF010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21926_length_4307_cov_4.116416"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2382)
                     /locus_tag="PKJ16_17030"
     CDS             complement(<1..2382)
                     /locus_tag="PKJ16_17030"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48749.1"
                     /translation="MALCALAWTACKGCGPLSSKEKRPVAVKGVIDLRNWDFERDGAI
                     DLNGEWEFCWDRELASTDFVRMGSREGCGHIAVPGSWRGHTHTGAPLPGAGRATYRLR
                     ILLSPSKRATTITVRRVFTAYRLWINGVLIDRKGSMGESPRTREDYPHVHDRRPSTFE
                     PRAGVNEIVLQVFNREHPGGGIDQPIRLGDAETTSRRAFLLHTFDMIIVGLLLFMVAY
                     NIMFYIFRRQDAATLYIGLSSLAWALNTYNIQHPLISDALGPVANPLLVNYLTVIPGP
                     ILFLMIVEALFPDEFSTTVIRISQVIAAGFALSLFFVDARTAERIMGVFYPIIFVWIT
                     YSLVGLIASIRRRRDDAVVFFAGLVFVYAGAVNNVLFILKFLDTGNVMPYAMVAFSLF
                     ATFVVSRRFARALSTVEEMSRELTEKNIALEKLDRLKDQFLANTSHELRTPLHGMIGI
                     AESMMEDAPGQLPPQARENLSLIASSGRRLATMVNDLLDMAKIHDEGLSLAPRPVDLQ
                     ALSAVAVRLSRPLAGDKPLEIVNRIEPDIPTVWADEDRIRQVLSNLLGNAIKFTAEGT
                     IELSARMIRVPDAGSGDDARAMVEVRVADTGIGVPEEHRERIFEAYRQADAGDTRSYG
                     GTGLGLAIAKQIVEAHGGAIALAPRDGGGSVFSFTLPVSHTQVRAAAGGPAMDAGPDL
                     PPADEPAGPFVLPAGISESQFDGSPVILVVDDDPLNLRIVQRYFEARQCVVKTAPDGI
                     RALDLIEKDHAIDLVLLDIMMPIMSGYEVCRRIRMLRSPEELPVIMLTAKNMMA"
     gene            complement(2699..>4307)
                     /locus_tag="PKJ16_17035"
     CDS             complement(2699..>4307)
                     /locus_tag="PKJ16_17035"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNV48750.1"
                     /translation="VTAAPNVGYHFTSWSDGGLIATRTDTNITGDLMVTASFSNEYTV
                     TFDAQSGSTPAPATKIVTYNAAYGALATTARDSYVFSGWWTGPNGTGTEIAAATIVLM
                     TSNHTLYAYWVPANMVFTSMRDGRSQIYVMNAGVQTNVSNNGWNDFNPHYSPDGSRIA
                     FMSTRTGNKEGFIMNADGTNPVNLTNNGADDSGGSWSPDGTKIVFHSNRWSGDRIYIM
                     NSDGSGTTILNPSGVNGGSAVWSPDGTKIAFNSTSGRIYVMNADGTNQQIVSSGLGDW
                     GPSWSPDNTKIAFSSTRTGNREIFVRNADGSGPETNLTNYVGADDDSPSWSPDGTKIA
                     FHSNRDGHYEIYLMGADGSRQTRITWGTSDNHFPSWAHTFANGVIGPGGGYIFYDKGF
                     YSTDGNGTWRYLEAAPVDQSAPMSMLWWNGASTTTGATGTAIGTGKTNTNAIISSQGA
                     GTYAATACTTYSNNGYNDWFLPSRDELIAIRNNLYVFGIGGLNWQDYVSSSEINSTTV
                     YYIYFDNGNTYTVGKNFPKTVRAIRAF"
BASE COUNT          820 a         1244 c         1362 g          881 t
ORIGIN      
        1 ggccatcatg ttcttggccg tgagcatgat cacgggcagc tcctcgggcg agcggagcat
       61 ccgtatgcgc cggcatacct cgtagccgga catgatgggc atcatgatgt ccaggagcac
      121 gaggtcgatg gcgtggtcct tttcgatgag gtccagcgcc cgtatcccgt ccggggccgt
      181 tttcacgacg cactgcctgg cctcgaagta gcgctggacg atcctgaggt tcagcgggtc
      241 gtcgtccacg acgaggatga ccgggctgcc gtcgaactgg ctttcgctga tccccgcggg
      301 aaggacgaac gggcccgccg gctcgtcagc cggcggaagg tccggtccgg cgtccatggc
      361 ggggccgccg gcggcggcgc ggacctgcgt gtgcgatacg ggcagcgtga atgaaaatac
      421 ggacccgccg ccgtctcggg gagccagggc gattgcgccg ccgtgcgcct ccacgatctg
      481 cttcgcgatg gccagcccca ggcccgtccc gccgtaggag cgcgtatcgc ccgcatcggc
      541 ctgccggtag gcctcgaaga tgcgctcccg gtgctcctcc ggaacgccga tcccggtgtc
      601 ggccacgcgc acctcgacca tggcgcgcgc gtcgtcgccg ctcccggcgt ccggcacgcg
      661 gatcatgcgc gcggagagct cgatggtccc ctcggcggtg aacttgatcg cgttgcccag
      721 cagattggag agcacctggc gtatgcggtc ctcgtcggcc cagacggtgg ggatgtcggg
      781 ctcgatgcgg ttcacgatct ccaggggctt gtcccccgcg aggggacgcg agaggcgcac
      841 cgccaccgcg ctcagggcct gcaggtccac cggccgcggc gcgagcgaga ggccctcgtc
      901 gtggatcttt gccatgtcca gcaggtcgtt caccatggtc gcgaggcggc ggccgctcga
      961 ggcgatcagg gagaggttct cgcgcgcctg cggcgggagc tgcccggggg cgtcctccat
     1021 catggattcg gcgatgccga tcatgccgtg cagcggcgtg cgcagctcgt gggaggtgtt
     1081 ggcgaggaac tggtccttga ggcggtccag cttctcgagc gcgatgttct tctcggtgag
     1141 ctcccgcgac atctcctcga cggtcgaaag cgcacgggcg aaccggcgcg agacgacgaa
     1201 ggtggcgaac agcgagaagg cgaccatcgc gtagggcatc acgttcccgg tgtcgaggaa
     1261 cttcaggatg aagaggacat tgttcacggc ccccgcgtac acgaaaacca ggccggcgaa
     1321 aaacaccacg gcgtcgtctc tgcgccgccg aatcgacgcg atcagcccca cgaggctgta
     1381 ggtgatccag acgaatatga tcggatagaa gacgcccatg atccgctccg ccgtcctggc
     1441 gtcgacgaag aacagcgaga gggcgaaccc ggccgctatg acctgcgaga tccggatgac
     1501 ggtcgtggag aattcatcgg ggaagagcgc ctccacgatc atgaggaaca ggatcgggcc
     1561 cgggatcacg gtgagataat tgaccaggag ggggtttgcg acagggccca gcgcgtcgga
     1621 aatcagcggg tgctgtatgt tgtaggtgtt cagggcccat gccaggctcg agaggccgat
     1681 gtacagcgtg gcggcgtcct gccgccggaa gatgtagaac atgatgttgt aggcgaccat
     1741 gaacagcagc aggcccacga tgatcatgtc gaaggtgtgc agcaggaacg cccggcggga
     1801 agtcgtctcg gcgtcgccaa gccgtatcgg ctggtcgatc ccgccgcccg ggtgctcgcg
     1861 gttgaagacc tgcagcacga tctcgtttac gcccgcgcgc ggctcgaacg tcgagggccg
     1921 ccggtcgtga acgtgcgggt agtcctcccg cgtccgcggc gattccccca tcgagccctt
     1981 gcggtcgatg aggaccccgt tgatccagag ccggtaggcc gtgaacaccc ggcggacggt
     2041 gatggtcgtc gcgcgcttgc tcggggaaag cagtatgcgc agccgatagg tcgcgcggcc
     2101 cgcgccgggc aggggcgcgc ctgtgtgcgt atggcccctc catgaaccgg gcacggcgat
     2161 gtggccgcag ccttcgcggc ttcccatgcg aacaaagtcc gtcgacgcaa gctcccggtc
     2221 ccagcagaac tcccattcgc cgttcaggtc gattgcgccg tcgcgctcga agtcccagtt
     2281 ccgaaggtcg atgacgccct tgaccgcgac gggccgcttc tccttcgagg acaacggccc
     2341 gcagcccttg catgcagtcc aggcgagagc gcacaaggcc aatgcgacgc acaaccttcg
     2401 catggatgat gggtggattg gcattgaccc attgggtgcg ggaaaaacag aatgtcaagt
     2461 acgatagggt cggtgcatca tgccggtagg gggcgtaacg gtcggcgctt gtgaaaaatg
     2521 ctttagattt ctcgcaatct ttccggaaga ccgtcttgtt gcgagaggcg gggcgcagcc
     2581 ctgtacatgt gatgcggcat tccctcgtct gtttctttgg agaaaacgca tgcaatcgat
     2641 gagcctgcct ggatagtgca ggatgcttga tgaaaaatta cagttcgcaa tcccatatct
     2701 aaaatgccct gatggcccga acagtctttg ggaagttctt tccgactgtg taggtgttgc
     2761 cgttgtcgaa gtagatgtaa taaacggtag tactgtttat ctccgatgag ctcacatagt
     2821 cctgccagtt gagacctccg ataccgaata cgtataaatt gttccgtatc gcgataagct
     2881 cgtctcgtga gggaaggaac cagtcattgt atccattgtt gctgtacgtg gtgcaagccg
     2941 ttgctgcgta tgttcccgct ccttggctgg aaataattgc gttcgtattc gttttcccgg
     3001 tgcctatcgc cgttccggtt gccccggtcg tcgtggaagc tccattccac cacagcatgg
     3061 acatcggtgc ggactggtca acgggggcgg cttccagata tcgccacgtt ccgttgccgt
     3121 cagttgaata aaagcccttg tcgtagaata tatatccgcc tccgggaccg ataacgccgt
     3181 tcgcaaaggt atgcgcccaa ctggggaagt gattatcgct cgtgccccat gtgatcctgg
     3241 tctgacggct tccatcggca cccatcaaat atatttcgta gtgtccatct cgattggagt
     3301 gaaaagcgat tttagtgccg tcgggagacc aggatggaga atcatcgtcg gctccaacat
     3361 agttggttag attggtttca gggccgcttc cgtcagcatt cctgacaaaa atctcccgat
     3421 tcccagtgcg agtggaggaa aaggcgattt tggtgttgtc gggagaccag gatggccccc
     3481 aatcgcccag accggatgaa acaatttgct gattggtccc gtcggcgttc ataacataaa
     3541 tacggcccga agtactatta aaggcgattt tagtgccgtc gggagaccaa actgcggaac
     3601 caccgttgac tccgctggga ttcagtattg tcgtcccgct tccatcgcta ttcattatat
     3661 aaatacggtc ccccgaccac cgatttgaat gaaagacgat tttagtgccg tctggggacc
     3721 agcttcctcc gctatcgtct gccccattat tggtgagatt gactggattg gtcccatcgg
     3781 cattcattat aaaaccttct ttattcccag tacgagtgct catgaatgcg atgcgggacc
     3841 cgtcgggtga gtaatgtgga ttaaaatcat tccatccgtt atttgaaacg ttagtttgaa
     3901 cgcccgcatt catgacatat atctgagaac gaccgtcgcg catgcttgta aagaccatgt
     3961 tggccggtac ccagtaagcg tacagcgtat ggttcgatgt cattaaaaca atggtagcgg
     4021 cggcgatttc cgttccggtc ccgtttggac ccgtccacca gccggaaaat acatagctgt
     4081 cgcgagcggt tgtcgcgagc gccccgtagg ctgcgttgta ggtgacgatt tttgtcgcgg
     4141 gcgcgggcgt gctcccgctt tgcgcatcga aggtcacggt atactcgttc gaaaacgagg
     4201 cggtcaccat gaggtcgccg gtaatgttcg tgtccgtccg cgtggcaata agccctccgt
     4261 cgctccacga ggtgaagtga tagccgacgt tcggcgcggc ggtgacg
//
LOCUS       DAORHF010000297         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22210_length_4271_cov_5.448055, whole genome shotgun sequence.
ACCESSION   DAORHF010000297 DAORHF010000000
VERSION     DAORHF010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22210_length_4271_cov_5.448055"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="PKJ16_17040"
     CDS             <1..1501
                     /locus_tag="PKJ16_17040"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV48751.1"
                     /translation="SSIVRFGLFNIDRIINRSFYIVVLLAVFTAVYAGFFVLLDGALG
                     GLFYGSGMIAGVIAFVATIVLYIVFSYQFEQLVYRLLFHNVYRVNSAIDSIIERTAAV
                     IRQDDIIDFVAGVLRDVIGAQFACIYLREQRPQGAYQAHFEHGENRRLLPASFDGASG
                     IVRHLGEAGRAVPVEYLEAADLAENVGDDAARLIHAAGIATAAPLYSQGRLIGFVLIG
                     KKTGGTLFGYYDIDFVNRLLHPIALAIENISLIDSYRREQVMTNEILIAKSIQSQLLP
                     PRLPAVPGYDIAAATHPAFDMSGDFYDVVVRDTGEVDCIVGDVSGKGVPAAFYSALVS
                     GIIAASTYAERASDMILQSMHRVITKRYIQRFYLAACYAILRPERHALEFTNAGLIYP
                     VRASADATEYLKSGGVPVGTRLPPRYTPHTVALEVGDLVVFATDGIIEATNADKEFFG
                     FERYLDTVRRFRELPARGLLEKLLGAVYEHIGDTGQDDDITIVIVKRVS"
     gene            complement(1515..2363)
                     /locus_tag="PKJ16_17045"
     CDS             complement(1515..2363)
                     /locus_tag="PKJ16_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016520619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48752.1"
                     /translation="MKTIDLSEKKLSLTNDGALELFFIGAGSAFTKRQYQTNLLIVKG
                     KDHLLVDCGTKCNQALFELGVPISSIRNFLITHSHADHIGGLEEVSLIGRYVSKKKPV
                     MIINETYQYILWDMSLRGGCAYNEEEARDMLGFSDFWDVVRPQWMPDYTRETFSATIG
                     AIDIKMMRTKHIPDSSSDWQSSFWSCGVIIDDRVMFTSDTRYDEDLVLSYDKRFNFEA
                     IFHDCQFYTGGVHASLEELKNLPADVKKKMYLSHYNDNWEEFEGTVREYGFADLAKQH
                     VYYAFP"
     gene            2584..3126
                     /locus_tag="PKJ16_17050"
     CDS             2584..3126
                     /locus_tag="PKJ16_17050"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HNV48753.1"
                     /translation="MKRALKLVAVFLVVIIAFGIFVNCYGSFALFHRIHKWNGTIGNQ
                     WLRSIVHFLMWFPLPVYPICLFIDWLILNNLEFFGFGNPMAMGPGEEETKVVVYNGKK
                     YRITVSQNRVDLEQLTGEQAGQMGALIFNPEDKSWSVENKLMMVREKVAQMDENDPNI
                     LHVVHPDGHTERVDMTTYLQ"
     gene            complement(3173..3661)
                     /locus_tag="PKJ16_17055"
     CDS             complement(3173..3661)
                     /locus_tag="PKJ16_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012674438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48754.1"
                     /translation="MSAQCAIILPLLSALLLASAGCGIVPKIVVMRDPLSAEEHNDLG
                     VAYERKGTYALAEDEYRAAQRKRTDWHLPAYNLGNLYYRMRRYDDAEERYRAAIALDP
                     RCSDCMNNLAWLLRERGRLAEARALAERALAVRNAPEYADTLRKIAEDEKRPSGKTPG
                     GR"
     gene            complement(3658..4134)
                     /locus_tag="PKJ16_17060"
     CDS             complement(3658..4134)
                     /locus_tag="PKJ16_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HNV48755.1"
                     /translation="MNAIAVIATLALLSAGMDVPFVRQKPHFCGPAALASVMAFFGDA
                     IGQDEIARAVYSPSLKGALVTDLENFARGRGYKTELRQGSLDDVRRHIAARRPVILLV
                     DRGLWFVSRPHYLVVFGRSGDSFIAHTGDHAGVAFSPAELRSLWDKAGNVLLVVFR"
BASE COUNT          746 a         1350 c         1361 g          814 t
ORIGIN      
        1 ctcgtccatc gtccgcttcg ggttgttcaa catcgaccgc atcatcaacc gcagctttta
       61 catcgtggtg ctgctcgcgg tgttcacggc cgtgtacgcc ggcttcttcg tcctgctcga
      121 cggcgcgctc ggcggcctct tctacggcag cggtatgatt gcaggcgtga tcgccttcgt
      181 cgcgaccatc gtgctctaca tcgtcttcag ctatcagttc gagcagctgg tgtaccgcct
      241 gctgttccac aatgtgtacc gggtgaattc ggcgatcgat tccatcatcg aacgcacggc
      301 ggccgtcatc cggcaggacg atatcatcga cttcgtcgcc ggggtgctgc gcgacgtcat
      361 tggggcgcag ttcgcctgca tctacctccg ggagcagcgg ccccagggcg cctaccaggc
      421 ccacttcgag cacggcgaga accggcgcct gctgccggcc tccttcgacg gcgcaagcgg
      481 catcgtgcgc cacctcggcg aggcgggccg ggcggtgccc gtcgagtatc tcgaggcggc
      541 cgatctcgcc gagaacgtcg gcgacgacgc ggcgcggctg atccacgccg ctgggatcgc
      601 gacggcggcg ccgctgtact cgcaggggcg gctcatcggc ttcgtgctca tcgggaagaa
      661 gacgggcggg acgctgttcg gctactacga catcgatttc gtgaaccggc tgctgcaccc
      721 catcgcgctc gccatcgaga atatctcgct catcgacagc taccggcgcg agcaggtgat
      781 gaccaacgag atcctcatcg ctaagagcat ccagagccag ctcctgccgc cgcgcctccc
      841 cgcggtgccg ggctacgaca tcgcggccgc gacccatccc gccttcgaca tgagcggcga
      901 cttctacgat gtcgttgtgc gcgacaccgg cgaggtggac tgcatcgtgg gcgacgtttc
      961 cggcaagggc gtgcccgcgg ccttctacag cgcgctggtg agcggcatca tcgccgcgag
     1021 cacctacgcc gagcgcgcca gcgacatgat cctgcagtcg atgcaccgcg tgatcaccaa
     1081 gcgctacatc cagcgcttct acctcgcggc ctgctatgcg atcctccgcc cggagcggca
     1141 cgcgctcgag ttcacgaacg ccgggctcat ctaccccgtg cgcgcgagcg ccgatgcgac
     1201 cgagtacctc aagagcggcg gcgtgccggt gggcacccgg ctccccccgc gctacacccc
     1261 gcacacggtc gcgctggagg tgggcgatct cgtggtgttc gcgaccgacg gcatcatcga
     1321 ggcgaccaat gcggacaagg agttcttcgg gttcgagcgc tatctcgaca cggtgcgccg
     1381 gttccgcgag ctgccggcgc gagggctcct ggaaaagctc ctgggcgcgg tgtacgagca
     1441 tatcggcgac acggggcagg acgacgacat caccatcgtg atcgtgaagc gcgtttcgta
     1501 gcggcggtca agggctacgg gaaggcgtag tagacgtgct gtttcgcgag gtcggcgaaa
     1561 ccgtactcgc gcaccgttcc ctcgaactcc tcccagttgt cgttgtagtg gctcagatac
     1621 atcttctttt ttacgtccgc ggggaggttc ttcagctcct cgagcgatgc gtgcactccg
     1681 ccggtgtaga actggcagtc gtggaagatc gcctcgaagt tgaagcgttt gtcgtaggag
     1741 agcaccaggt cctcgtcgta gcgcgtatcg ctggtgaaca tcacgcgatc gtcgatgatc
     1801 acgccgcagc tccagaacga gctctgccag tcggaggagg aatccgggat gtgcttggtg
     1861 cgcatcatct tgatgtcgat ggcgccgatc gtcgcgctga aggtctcgcg ggtgtagtcc
     1921 ggcatccact gaggtcggac cacgtcccag aagtcgctga agcccagcat gtcgcgcgcc
     1981 tcctcctcgt tgtaggcgca cccgccgcgc agggacatgt cccagaggat gtactggtag
     2041 gtctcgttga tgatcatcac cggctttttc ttggagacgt agcggccgat gagcgatacc
     2101 tcctcgagtc cgccgatgtg gtcggcgtgc gaatgcgtga tgaggaagtt ccgtatcgag
     2161 ctgatgggca cccccagctc aaaaagcgcc tggttgcact tggtcccgca atcgacaagc
     2221 aggtggtcct tccccttgac gatgagcagg ttcgtctggt actgtcgctt ggtgaaggcg
     2281 ctccccgcgc cgataaagaa gagctccagc gcgccgtcat tggtcaggga aagtttcttc
     2341 tcgctcaggt cgatggtctt catattccgc cttccagggg gatgatttct ttcggtgcgg
     2401 atatctatat tgcctctgcc gcatcggctt gagcgtatac aggattacat atttgtcaac
     2461 caactttgta gcatcacggc gaaatcggcg ggaagggcgg gcgcgcgtcg cggcaaaaat
     2521 gttgttgcat tttcacgccg atgccgtatc cttccggcaa tcaatcatgg aggactccaa
     2581 cgtatgaaaa gagcattaaa gcttgtcgcg gtctttctcg tcgtcatcat cgccttcggc
     2641 atcttcgtga actgctacgg cagcttcgcg ctcttccacc gcatccacaa gtggaacggg
     2701 accatcggca accagtggct acggtccatc gtccactttc tgatgtggtt cccgcttccg
     2761 gtctatccga tatgcctgtt catcgactgg ctcattctga acaacctcga gttcttcggg
     2821 ttcggcaacc cgatggcgat gggccccggc gaggaggaga ccaaggtggt cgtctataac
     2881 ggcaagaagt accggatcac cgtgagccag aaccgcgtgg acctcgagca gctcaccggc
     2941 gagcaggccg gacagatggg cgcgctcatc ttcaatcccg aggacaagtc ctggtcggtc
     3001 gagaacaagc tcatgatggt tcgcgagaag gtcgcgcaga tggacgagaa cgatccgaac
     3061 attctgcacg tggtccatcc cgacgggcat accgaacggg tcgacatgac cacctacctg
     3121 cagtaatttg cgcaacgcgc acgataaaaa accccgccat cggcggggtt ttttatcggc
     3181 cccccggcgt tttccccgat gggcgctttt cgtcctccgc gattttccgg agcgtgtcgg
     3241 cgtactccgg cgcgttcctg acggccagcg cgcgctcggc gagcgctcgg gcctcggcga
     3301 gccggccgcg ctcgcgcagc agccaggcaa ggttgttcat gcagtccgag cagcgggggt
     3361 cgagcgcgat ggccgcgcga tagcgctctt cggcgtcgtc gtagcgccgc atgcggtaat
     3421 acaggttgcc caggttgtag gccggaaggt gccagtccgt ccgcttgcgc tgcgccgcgc
     3481 ggtattcgtc ctcggcgagc gcataggtcc ccttccgctc gtaggccacg ccgaggtcat
     3541 tgtgctcctc ggcgctcagt ggatcgcgca taacgacgat cttcgggacg atcccgcatc
     3601 cggcgctcgc gagaagaagc gcggaaagca gcgggaggat gatggcgcat tgcgcgctca
     3661 tcggaacacc accaggagca cgttgcccgc cttgtcccac agcgagcgca gctccgcggg
     3721 cgagaatgcg acgccggcat ggtcgccggt gtgagcgata aacgaatcgc cggagcggcc
     3781 gaagacgacc agatagtgcg gccggctcac gaaccacagg ccccggtcca ccaggaggat
     3841 gaccggccgc cgcgccgcga tatggcgccg cacatcgtcg agcgatccct ggcgcagttc
     3901 cgttttgtac ccgcgccccc gtgcgaagtt ctcgaggtcg gtcaccagcg cccccttcag
     3961 cgatggggaa tagaccgcgc gggcgatttc gtcctgtccg atggcgtcgc cgaaaaacgc
     4021 cattaccgag gcgagcgcgg cggggccgca gaagtgcggc ttctggcgga caaagggcac
     4081 gtccatgccg gcggaaagga gtgcgagcgt ggcgatgacg gcgatcgcgt tcacgccgat
     4141 cggcgcccgg tttcggcgtg gggtgtcagc acggcgcgag gcgcgggcgt ggcgtcacct
     4201 cttcttgtca aggaagagcg cgagtatgac gatgacgatg gcgacgatga tgatgagccc
     4261 gcccaccgag a
//
LOCUS       DAORHF010000298         4270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22224_length_4270_cov_4.551601, whole genome shotgun sequence.
ACCESSION   DAORHF010000298 DAORHF010000000
VERSION     DAORHF010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22224_length_4270_cov_4.551601"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..1347)
                     /locus_tag="PKJ16_17065"
     CDS             complement(73..1347)
                     /locus_tag="PKJ16_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48756.1"
                     /translation="MMARIHVRIAIAVACCVTLLAGWALAKDVDAEAPPRTPLQLALL
                     NPMQLFDQDYDVYGLRINLLYGSNTSVYGLDLGLFNDCVRDLYGIQAGIIGNMVSGVG
                     SSGDGLLGVQIAGLFNYAESRASGHGPRGIQIAGICNYTPGAKGYFQAAGIFNLATDA
                     DFTGVQVAGLLNIGVKTMTGLAIAGLVNHTTAGFAGIRAGGWGNYTEGDMAGVQIAGV
                     INVTVRGSVSGLEAALICNASYNEHYRVDKPTGDPTLRGIQLAGLYNDGHAMLTGAQI
                     TLGGNMAGYFAAAPEAGNSFLQLAGGFNYCVRKFTGAQVAGVLNLADSLSGVQVAGVA
                     NIIYGDSGSLTGRREGDLRGMQVSLGLNSTNLDVYGCQVGLINVADRVKGVQIGLVNI
                     ARRVYGVQIGLANFISEGAVPFFPIVNFSTSF"
     gene            complement(1360..1896)
                     /locus_tag="PKJ16_17070"
     CDS             complement(1360..1896)
                     /locus_tag="PKJ16_17070"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV48757.1"
                     /translation="MAGIFEITKDRKGEWRFALKATNGEIIATSEGYKTLESCRKGIA
                     SVVRNATGARIRNRDADAALIDAADEGDLDGIRRALKNGADVNCRDEEGETPLIEAVE
                     SGNLEAVKHLVAKGAKLNLQDAEGETALHEAIEEGHAEIIRFLVRAGARRNLKDKKGR
                     TPVALAAQKGIELPSGKQ"
     gene            2322..2900
                     /locus_tag="PKJ16_17075"
     CDS             2322..2900
                     /locus_tag="PKJ16_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HNV48758.1"
                     /translation="MNVQEAIAKRRAYRALDPVEIPDETVRELAGAAQLMPSCFNNQP
                     WRFVFARSPEALERMRRALNKGNEWAHHASTIVAAFARREHDCLIKEREYYLFDLGMA
                     VSAMLLRATEMGLVAHPIAGYDPDEVRRALGIPDDCMVITLIIIGKKSDDLSALSEWQ
                     AAQEPQRPVRLPLETIYSIDRFDERMNAKVER"
     gene            complement(3023..>4270)
                     /locus_tag="PKJ16_17080"
     CDS             complement(3023..>4270)
                     /locus_tag="PKJ16_17080"
                     /inference="COORDINATES: protein motif:HMM:NF027798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,3-glucanase family protein"
                     /protein_id="HNV48759.1"
                     /translation="GGGSKDGPFDTFITCEFVNSTSGGETYGNDKIWVTAYSKDESGA
                     FFSIDLNDDGVGSRVYAKAAYQQLISRNLEYISARGGLKLPVYTNGARLYISYGDSAI
                     FIQYVDTPVGLGVEGPAIANGEWGTKIWDFIEFAAVKPDGVDFNVNTSTVDSFCIPIQ
                     IELKNAIGTSLGTRGINKSRSYVFSQWEASTMPDAFKKLVYKDGGTYIRIISPGPIAT
                     AASISADGDFKYLKDYLHNLDGTSLVGIQNSLKEFLNSTLNTMWGNHTSSGAGLSFVD
                     GGKTYTCWAEGSDWLFSTDGGSTNDKIVNLKVGSDSSCIWGCSGPLECQPGEDEKNTV
                     KKFLAAAFNRGMVTQGWDDTDGSKYYKPDGSGGVYNHYSKFFHRTDVSPDGKAYGFSF
                     DDVHDKNSSIGNASTKKMIITVR"
BASE COUNT          876 a         1327 c         1155 g          912 t
ORIGIN      
        1 gatcggcctg gccaacttca tctcggaggg ggcggttccc tttttcccga ttttcttttt
       61 catggtgatc cgctaaaacg acgtgctgaa attgacaatc gggaaaaagg gaaccgcccc
      121 ctccgagatg aagttggcca ggccgatctg cacgccgtac acgcggcgcg cgatgttcac
      181 gagcccgatc tgcaccccct tcacccggtc ggcgacgttg atcaggccca cctggcagcc
      241 gtacacatcg aggttggtcg aattgaggcc caggctcacc tgcattccgc gcagatcccc
      301 ctcgcggcgc ccggtgaggg agcccgaatc gccgtatatg atattcgcga cgcccgcgac
      361 ctgtacgccg gacaggctat cggccaaatt gagaacgccc gcaacctgcg cccccgtaaa
      421 ctttcgcacg cagtaattga atcctccggc aagttgaaga aaggaattgc cggcttcggg
      481 cgcggcggcg aaatacccgg ccatattgcc ccccagggtg atctgggcgc ccgtgagcat
      541 cgcgtgaccg tcattatata atccggccag ctgaatcccc cgcagggtgg gatcgcccgt
      601 tggcttgtcg acacgatagt gctcattgta gctcgcattg cagattaaag cggcctcgag
      661 cccgctcacc gatccccgca cagtcacgtt gatgacgcca gcgatctgga cgcccgccat
      721 gtcgccctcc gtataattgc cccagccacc cgcccggata ccagcaaagc cggcggtggt
      781 gtggttcacg agaccggcga tagccagtcc cgtcatggtc ttgacgccta tgttcaagag
      841 ccccgcgact tgcaccccgg tgaaatcggc gtcggtggcc aggttaaaga tgccggccgc
      901 ctggaagtat cccttcgcac cgggcgtgta gttgcagatc ccggcaatct gaatcccccg
      961 cggcccgtgc cccgacgcgc gcgactccgc gtaattgaac aaccccgcga tctgcacccc
     1021 caacaggccg tcgcctgacg agccaacccc gcttaccata ttgccgataa tcccggcctg
     1081 aatgccgtag aggtcccgca cgcaatcgtt aaacaggccc aggtcgagcc catagacgct
     1141 ggtattgctg ccatacagaa ggttgatccg cagcccgtag acgtcgtagt cctgatcgaa
     1201 gagctgcatg gggttaagga gcgcgagctg cagcggcgtg cgcgggggag cctccgcgtc
     1261 aacatccttg gcgagcgccc atccagccag gagcgttacg cagcacgcca cggcaatcgc
     1321 gattcgcaca tggatgcggg ccatcatcaa accgaggccc tactgctttc ccgacggcag
     1381 ctcgatcccc ttttgagcgg caagcgcgac cggcgtgcgc ccctttttgt ccttgagatt
     1441 gcgccgggcc ccggcgcgca ccaggaagcg gatgatctcc gcatgcccct cctcgatcgc
     1501 ctcgtgcaac gcggtttcgc cctccgcgtc ctgcagattg agcttcgcgc ccttcgcgac
     1561 gaggtgcttc acggcctcca gattgccgga ctcgacggcc tcgatgagcg gcgtctcgcc
     1621 ctcctcgtcc cggcagttca cgtccgcgcc gtttttgagc gcccgccgga tgccgtcgag
     1681 gtcgccctcg tccgcggcgt cgatgagcgc cgcgtcggcg tcgcgattgc ggatgcgcgc
     1741 gccggtcgca ttgcgcacca ccgaggcgat ccccttccgg cagctctcga gggttttata
     1801 cccctcgctc gtcgcgatga tctcgccatt ggtcgccttg agggcgaagc gccactctcc
     1861 ctttcggtct ttcgtgattt caaatatacc ggccatgtgg ttcctccctg tggtattact
     1921 gtcacttcac gcgcatcgac gtatcgtcag gtcatctcat ttcgcattgc ttcgatcgcc
     1981 gtcttgattg aaagaatgtg attcgaatca tagcgaggag cgatagcgac gaagaaatcc
     2041 agcacactct tagggggatc aaacttgtac cggatgctca taaagtgccg cttattgtaa
     2101 cagatcgctt cgtttcgctc gcgatgacat cgagcaaatc aacacgtttg atataattgc
     2161 atacgatacc cggccccgcc ccgtcaatta tttttgcatg ggacattcca gcacggaaat
     2221 cttggaacat ctcgaaaaat ggagtatgtt ttctatgcgc atacgggcca aggtgccccc
     2281 aaccgcgctc ggcgacacaa cgaaatacgg gaggatccgc catgaacgta caggaagcca
     2341 tcgcgaaacg cagggcgtac cgggcgctcg atccggtcga gatacccgac gagaccgtca
     2401 gggagctcgc gggcgctgcg cagctcatgc cgtcgtgctt caacaatcag ccgtggcgct
     2461 tcgtattcgc ccgctcgccg gaggcgctgg agcggatgcg ccgggccctg aacaagggca
     2521 atgagtgggc gcaccacgcc tcgaccatcg tcgccgcctt cgcgcggagg gagcacgact
     2581 gcctcatcaa ggagcgggag tactacctgt tcgatctggg gatggcagtc tcggccatgc
     2641 tgctgcgcgc cacggagatg ggccttgtgg cgcaccccat cgcgggctac gatcccgacg
     2701 aggtccgccg cgcgctgggc attcccgacg actgcatggt gattaccctg attatcatcg
     2761 ggaagaagtc ggacgatctg agcgcgctct cggaatggca ggccgcccag gagccgcagc
     2821 gtccggtgcg gcttccgctc gagacgatct acagcatcga ccgcttcgac gagcggatga
     2881 acgcgaaggt ggagcggtag gacctcaccc cctgcccctc tcccaaagga gaggggggaa
     2941 tattgccata atcagtgaaa cgcacatggc gcgtggggca tccccggaag atgcgccatg
     3001 ctgttaccga ctcggcggcg aactaccgca cggtgattat catttttttt gtggacgcat
     3061 ttccaatgga agaattttta tcgtgaacgt cgtcaaagga gaaaccgtac gctttcccat
     3121 cgggactcac gtccgttcta tgaaagaact tcgaatagtg attgtacacc ccgccagagc
     3181 catcgggttt gtaatacttg gagccgtcgg tatcgtccca accttgtgtc accatacccc
     3241 ggttgaaagc ggcggccagg aatttcttga ccgtgttttt ttcatcttca ccgggctggc
     3301 attccaaggg accgctacag ccccagatgc aggatgagtc cgatccgact ttcagattaa
     3361 caattttatc atttgtagag ccaccatcgg tcgaaaaaag ccaatcactg ccttcggccc
     3421 agcatgtata tgtttttcca ccatccacga aagaaagacc agctccggac gacgtatggt
     3481 ttccccacat cgtattgagc gtgctgttca agaattcctt taacgaattt tgaattccca
     3541 caagcgacgt tccgtcaagg ttatgaaggt aatctttaag atacttaaag tccccgtcgg
     3601 ccgatatgct cgcagctgtg gcaatcggtc ccggcgagat aattcgtata taggtaccgc
     3661 catccttgta caccagcttt ttgaatgcat ccggcatggt agacgcttcc cattgcgaaa
     3721 acacataact tctcgatttg tttatccctc gcgtgccgag ggatgtcccg atggcgttct
     3781 taagttctat ctggatggga atgcaaaacg aatcgaccgt cgaggtgttt acgttaaaat
     3841 ccactccatc gggttttacc gcggcgaatt ctatgaagtc ccagattttt gtgccccatt
     3901 ccccgttcgc tatcgccggc ccctccactc ccaaaccgac tggggtatca acgtactgaa
     3961 tgaatattgc cgaatcccca taggaaatat acaatcttgc gccgttcgta tagacgggca
     4021 gctttagacc tccccgggca gatatatatt cgagattgcg gctaatcaac tgttggtatg
     4081 ctgctttggc atatacgcga ctcccgaccc catcgtcatt gagatcgatg gaaaagaacg
     4141 ctccgctttc gtccttggaa tacgccgtca cccaaatctt atcgttgcca tacgtctcgc
     4201 cgccgctcgt actgttaacg aactcgcagg taataaacgt atcaaacgga ccgtccttgc
     4261 tgccgccgcc
//
LOCUS       DAORHF010000299         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22336_length_4256_cov_5.072364, whole genome shotgun sequence.
ACCESSION   DAORHF010000299 DAORHF010000000
VERSION     DAORHF010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22336_length_4256_cov_5.072364"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PKJ16_17085"
     CDS             <1..1049
                     /locus_tag="PKJ16_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48760.1"
                     /translation="ADIGWVTGHSYIVYGPLANGATSIMFEGVPSYPDFGRFWAVCEK
                     WKVNIFYTAPTAIRAIAKEGDSWVDKHNIKSIQLLGSVGEPLNPEAWNWYFNKIGRGE
                     CPIVDTWWQTETGGILITPLPGAIDIKPAMATVPFFGVKPSIVDDAGKPLEGVCEGNL
                     CIDMSWPGQMRGVYGDPERFFNTYFVQFPGKYFTGDGCRRDKDGYYQITGRVDDVINV
                     SGHRMGTAEVESALVSHPKVAESAVVGYPHEIKGQSIYAFVTLNTGVEKTDELKKELV
                     AHVRKEIGPIATPEIIQWADALPKTRSGKIMRRILKKIAAADYDKKGYGDISTLADPT
                     VVDVLMDGSKAIKR"
     gene            1276..2610
                     /locus_tag="PKJ16_17090"
     CDS             1276..2610
                     /locus_tag="PKJ16_17090"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48761.1"
                     /translation="MKRTLIALIACMCAAANLSAGAKLKKMNVLVYPFANTGDAAFAW
                     ISAGMTDTVIADLKTLSGMNVFSEEDRKLAVHEMELAMAGLIPEKQAVSVGKIMGAHM
                     IFTGSYTVAGGAIRIIAKLQNVETTRVEKTLKIDGSLSDIFRVQDQVVLQLLDETKRV
                     VLPGVATVEVRPGEQQAITQKPAINVSAYEWYSKGLAVHDVNRDQALDFYSKAVALQP
                     DYSAALNRIGMTYTAKKDIGAALDALARAEQSLVKGNRQNSVLFANVLNSFGMAYFSK
                     KEYDRSLAFFNRSREVMEGLGKKQSRDYAVLMNNIAAVHFGTRDIPRTISYLDAALAI
                     LDAIGSRDTPQYARFSHNLGVMQASRKEYARALRSYAAAREIAERNGFTNRVWYGKIL
                     QSQAIALIKTGKSCDAVSPARASLAIFERAKDADASKARATLNAATKACAKK"
     gene            complement(2614..3243)
                     /locus_tag="PKJ16_17095"
     CDS             complement(2614..3243)
                     /locus_tag="PKJ16_17095"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HNV48762.1"
                     /translation="MEDFANRLIEYFGKAPDAALYLFLFASSVVENIFPPIPGDTITA
                     FGAFLVGTGRLNYGLVFAVTTVGSVVGFMTLFALGRYLERHFFIERDYTYFSAKSIIA
                     AEEWFRRRGNLVVLGNRFLPGVRSVISIVAGISMLNPLTVLLLSTVSAAVWNLIWIHA
                     GYMLGDRWEIVREKVGALMRSYNIVAAIVIVIAIIAVILYRWRKRPPEA"
     gene            3382..3948
                     /locus_tag="PKJ16_17100"
     CDS             3382..3948
                     /locus_tag="PKJ16_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48763.1"
                     /translation="MTLRKIAFTRTLPIVLGLAVSLASVACASKGAVPGAKAPDPIEE
                     FLKVDPESKELFRVVLMSDKYRIAQMSHQGTIKRMHDPGGDSFMAEELIKLDKIDEVR
                     FGTVKIWLYPTSGKVMKVRPFELTNLLEIDKLLVDDVQRWSFEFPGGSVQPQSFSIRY
                     MVVLRKKLTDGAILDQIRKDMSGKKTWR"
BASE COUNT          885 a         1280 c         1291 g          800 t
ORIGIN      
        1 cggccgacat cggctgggtc accggccact cgtacatcgt ctacggtccg ctcgccaatg
       61 gcgcgacctc gatcatgttc gaaggcgttc cgagctaccc ggatttcggc cgcttctggg
      121 ccgtctgcga aaagtggaag gtgaacatct tctacaccgc cccgaccgcc atccgcgcca
      181 tcgcgaagga aggcgattcg tgggtcgaca agcacaacat caagtccatc cagctcctcg
      241 gttccgtggg cgagcccctg aaccccgaag catggaactg gtacttcaat aagatcggcc
      301 gcggcgagtg cccgatcgtc gacacctggt ggcaaaccga gacgggcggc atcctcatca
      361 ccccgctccc cggcgccatc gacatcaagc ccgccatggc caccgttccg ttcttcggcg
      421 tgaagccgtc gatcgtggac gatgccggaa agcccctcga gggcgtgtgc gaaggaaacc
      481 tctgtatcga catgtcatgg cccggccaga tgcgcggcgt gtacggcgat cccgagcgct
      541 tcttcaacac ctacttcgtc cagttccccg gcaagtattt caccggtgac ggctgccgcc
      601 gcgacaagga cgggtactat cagatcaccg gccgcgttga cgacgttatc aacgtatccg
      661 gccaccggat gggtaccgcc gaggtcgagt ccgccctcgt gtcgcacccg aaggtcgccg
      721 agtcggccgt tgtcggctac ccgcacgaga tcaagggcca gtcgatttac gccttcgtga
      781 cgctgaacac cggcgtcgag aagaccgacg agctcaagaa ggaactcgtg gcccacgtcc
      841 gcaaggagat cgggcccatc gccaccccgg aaatcatcca gtgggccgat gcgcttccca
      901 agacccgttc gggcaagatc atgcgtcgta tcctcaagaa gatcgccgcc gccgactacg
      961 acaagaaagg ctacggcgac atctcgacgc tggccgatcc gaccgttgtc gatgttctca
     1021 tggacggctc gaaggccatc aagagatagt atctcttttt cacgcgaatc atcaaagggg
     1081 cggcatggtg tacatgccgc ccctttttcg tgcgtatggc agaggcgcac ccgtatcctg
     1141 cttggtcgtc ggcacacaat cgatcgatag tttcttcatt gacgagccgt gacacgcggg
     1201 tacggtttgt tcgcgaacat gccgcggcca tcgtcgcgaa cggctgtcat gtgcgttgtg
     1261 aatgcggagg ctgttatgaa acgaaccctg attgcgctta tcgcctgcat gtgcgccgcg
     1321 gccaatctct cggccggcgc gaaattgaaa aaaatgaacg ttctggtgta tcccttcgcc
     1381 aatacaggcg atgcggcctt tgcctggatc tcggcgggga tgaccgacac ggtcatcgcc
     1441 gatctgaaaa cgctgtccgg gatgaacgtg ttctcggagg aggaccggaa gctcgcggtc
     1501 cacgagatgg agctggcgat ggcgggcctc atccccgaaa agcaggccgt ttcggttggc
     1561 aagatcatgg gcgcgcacat gatcttcacc ggcagctata cggtcgccgg cggcgcgatc
     1621 cgcatcatcg ccaagctgca aaacgtcgag acgacgcggg tcgagaaaac cctgaagatc
     1681 gacggatcgc tcagcgacat cttccgcgtt caggaccagg tggtgctcca gctgctcgac
     1741 gagacgaagc gggtggtcct cccgggcgtc gcgacggtcg aggtgcgtcc cggcgagcag
     1801 caggccatta cgcagaaacc cgcaatcaac gtctccgcat acgaatggta ctcgaagggc
     1861 ctggcggtcc acgatgtgaa tcgcgatcag gcgcttgact tttacagtaa agcggtcgcc
     1921 cttcagcccg attactcggc ggccctgaac cggatcggca tgacctatac cgcgaaaaag
     1981 gacatcggcg ccgccctgga cgcgcttgcg agggccgagc agtcgttggt gaaggggaat
     2041 cggcagaatt ccgtgctgtt cgcgaatgtg ttgaacagct tcgggatggc gtacttttcg
     2101 aagaaggaat acgatcgctc gcttgcgttt ttcaatcgct cccgcgaggt catggagggg
     2161 ctcgggaaaa aacagtctcg cgactatgcc gtcctcatga acaatatcgc cgcggtccat
     2221 ttcggcacgc gcgacatacc ccggacaata tcgtatctgg atgcggcgct tgcgatcctt
     2281 gacgcgatcg gctcacgcga cacgccccag tacgcgcgat tttcgcataa cctgggcgtc
     2341 atgcaggcgt cgcggaagga gtacgcacgc gcgctcaggt cgtacgcggc agcgcgcgag
     2401 atcgccgaac ggaacggttt taccaaccgg gtctggtacg ggaaaatact ccagtcgcag
     2461 gcgatcgcgc ttattaaaac cggaaagagc tgcgatgcgg tttcaccggc gcgcgcaagc
     2521 cttgcgatat tcgagcgcgc caaggacgcg gatgcgagca aggcccgcgc gacgctcaat
     2581 gccgcgacga aggcctgcgc gaaaaagtga accctacgcc tccggcgggc gttttcgcca
     2641 ccggtagagt ataacggcga tgatggcgat gacgatgacg atggcggcga cgatgttgta
     2701 gctgcgcatg agcgccccca ccttctcccg aacgatctcc cagcggtcgc ccagcatgta
     2761 tccggcgtgt atccagatga ggttccatac cgccgcgctc accgttgaaa ggaggagcac
     2821 cgtgagcgga ttgagcatgg agatgcccgc gacgatggag atgacggagc ggaccccggg
     2881 caggaagcgg ttgcccagca caacgagatt cccgcggcgg cggaaccatt cctcggcggc
     2941 gataatgctt ttcgccgaaa agtatgtgta gtcgcgctcg atgaagaagt gccgctcgag
     3001 atagcgcccg agcgcgaaga gggtcatgaa gccaaccacg ctccccacgg tggtcactgc
     3061 gaacaccagg ccgtagttga gccggccggt ccccaccaga aaggcgccaa aggcggtgat
     3121 ggtgtcgccg ggaatgggcg gaaagatgtt ttcgaccacg gaactggcga acaggaagag
     3181 gtagagcgcg gcatcggggg ccttgccgaa gtattcaatg agccgatttg cgaaatcttc
     3241 catgggcgct cccagtagaa aaaatgtgtt gaaactattc tttggcaact ctattttgca
     3301 agtaaaatac ggcgcttcat tccacgcacc cttgtttgcc ggcttgcgcg gaggtgcggc
     3361 gccacgagat ggaagatgtt catgacactg cgaaaaattg cattcacccg caccctgcca
     3421 atcgtccttg gtcttgccgt ttcgcttgcg tcggtcgcct gcgcgtcaaa gggcgccgtt
     3481 ccgggcgcga aggcccccga tcccatcgag gaattcttga aggtggaccc ggaatcgaag
     3541 gagctgttcc gggtggttct catgtcggac aagtatcgga tcgcccagat gagccatcag
     3601 gggacgatca aacggatgca cgatcccggc ggcgacagct tcatggccga ggaactcatc
     3661 aagctcgaca agatcgacga ggtccgcttc gggacggtga agatctggct ctatccgacc
     3721 agcggaaagg tcatgaaggt gcgcccgttc gagctcacca acctgctcga gatcgacaag
     3781 ctccttgtcg atgatgtcca gcgctggagc ttcgaatttc cgggaggctc ggtgcagccg
     3841 cagagcttct cgatccgcta catggtggtg ttgcgcaaga agctcaccga tggcgccatc
     3901 ctggaccaga tccgcaagga catgtcgggg aagaagacct ggcgctgacc tcacacgctt
     3961 cgacaggctc agcgcgacgc ccgtcccctc tcctccggga gaggggcgca cagcgcacaa
     4021 tttaaaatta aatctattcc ccccttctcc tgcgggagaa ggggccgggg gatgaggtca
     4081 catcgacgtc gtattgatga tgaaccggat cgagttctcg tcgatgagga gcttcttcgc
     4141 gatctcgttc gatgaccagc cctttcgcga caattcccgc accgtccgga ccattccctc
     4201 gttgatgctc ttgtcgatgg gcagctcgcc cttcatttgc ttggcgatgg gcccgg
//
LOCUS       DAORHF010000300         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22337_length_4256_cov_4.983099, whole genome shotgun sequence.
ACCESSION   DAORHF010000300 DAORHF010000000
VERSION     DAORHF010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22337_length_4256_cov_4.983099"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKJ16_17105"
     CDS             complement(<1..418)
                     /locus_tag="PKJ16_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010508931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNV48764.1"
                     /translation="MNLADYIRERRARRGILLMTHAVVGYPSLEANRRMLDAMISADV
                     DLVELQLPFSEPVADGPLFVKANLDALAGGASHGQYWNLFSETAARAPFPVLAMCYYN
                     SAFRMGHAAFCERLRGSGGRGFIIPDLPREEYGDLEA"
     gene            complement(415..1653)
                     /gene="trpB"
                     /locus_tag="PKJ16_17110"
     CDS             complement(415..1653)
                     /gene="trpB"
                     /locus_tag="PKJ16_17110"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010153878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HNV48765.1"
                     /translation="MTQELFDFTKPGYYGQYGGAFVPEILRATLDELIAAFDAARADD
                     QFWGAYVRELQTYSCRPTPVTPLPNLTKKLGGATICCKREDLNHTGAHKVNNVIGQGL
                     LAKRMNKTRVIAETGAGQHGVATATMAARFGFECTIYMGAVDVRRQRPNVFWMEQLGA
                     TVVPIEDGSATLKDAINACLRDWSESMDSTHYVLGTACGPHPFPHMVSYFQSIIGQEA
                     RGQMLTQSGRLPDRVYACVGGGSNALGMFVPFIKNPEVELVGVEAGGHGIESGRHAAR
                     IPAHDIGVAQGYKTLFLQDDEGQMRETHSVAAGLDYIGVSPILAHLADTGRVRFTAAR
                     DDEAIAAAKLLIATEGIIPALESSHAFAGAIREAPSMRPDEIVLINLSGRGDKDIFTI
                     ADAIGDERWFSYLREMGARR"
     gene            complement(1664..2293)
                     /locus_tag="PKJ16_17115"
     CDS             complement(1664..2293)
                     /locus_tag="PKJ16_17115"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HNV48766.1"
                     /translation="MKTLLKICGITSLDDACMAIDEGADMLGFNFFRESPRYIDPKTA
                     AGIIEHLPSVAMCVGLLVRPTLADALEAVATSGVRFLQIYDPVDIPDYSALPVPVIHA
                     RRIGDGTPFSAQMHGAAMLLIDTHSKSALGGTGKTFDWDLIPASFPRERLALAGGITV
                     DNIAEALERVNPGVIDVASGAESAPGRKDRMKMRRLSIAILKHNLNTLT"
     gene            complement(2277..3077)
                     /gene="trpC"
                     /locus_tag="PKJ16_17120"
     CDS             complement(2277..3077)
                     /gene="trpC"
                     /locus_tag="PKJ16_17120"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015837542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HNV48767.1"
                     /translation="MTILDRIIEHKRGEVAERKRRAPLSALIDAERYRRATVSFRDAL
                     ARDDRFHFICEIKKASPSKGIIRHDFDPVRQARSYHDGGASAISVLTDEKFFMGSLDH
                     LSAIRETVGLPILRKDFIIDPYQIHEARAAGADMILLIARVLTSSQIDEYAALARGLG
                     MDVLLELADESDIDKLPSCDTIIVGINNRDLATFAVDINRSVSLRRLLPADALVIGES
                     GITGSDDCRLLKANGIRGALVGKTFMRSSDPAALLRRIIDEVNDEDAS"
     gene            complement(3082..>4256)
                     /gene="trpD"
                     /locus_tag="PKJ16_17125"
     CDS             complement(3082..>4256)
                     /gene="trpD"
                     /locus_tag="PKJ16_17125"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HNV48768.1"
                     /translation="SHPTFGVQFHPESILTTHGMQIIANWLAITAEHAAANAKAAVPA
                     IAKPAGENAIRPFLEKITAGKDLAREEASCVLEKIIAGDASETEIGALLVGLRIKGET
                     VDELVGFVDTMERHMDAVSLADADAIDVCGTGGDSSFTFNVSTTAALVIAAGGVTVAK
                     HGNRSISSKTGSADLLEALGVKIDLNSAQVKRCIDEIGIGFFFAPLFHPAMKAVAPYR
                     KKLGLRTFFNMLGPMLNPARVTRQLVGAFNLETAGKIAAVLQARGSRKVCAVHGNDGL
                     DEISIFADTTVFETAPGVMAPRRYEYRPPRANATASLAQVKGAGIDDNIRMTMQVLQG
                     ERSTARDMTVVNAAFGFYVAGRASSIEDGIRMAEESIDSGAALKKLSQFKDFSLRI"
BASE COUNT          703 a         1373 c         1393 g          787 t
ORIGIN      
        1 gggcctccag gtcgccgtat tcctcgcggg gcaggtcggg tatgatgaag ccgcgcccgc
       61 cggatccccg cagccgttcg cagaaggccg cgtgccccat gcgaaaggcc gagttgtagt
      121 agcacatcgc cagcaccggg aagggggcgc gcgcggccgt ttcggagaag aggttccagt
      181 actgcccgtg tgaggcgccc ccggcgagcg catcgaggtt ggccttcacg aagaggggcc
      241 cgtcggccac cggttcgctg aagggcagct gcagctcgac gagatcgacg tccgccgaga
      301 tcatggcgtc gagcatgcgc cggttcgctt ccaatgacgg atagccgacg accgcgtgcg
      361 tcatcaacaa tataccgcgc cgtgcgcgcc gttcgcgaat gtagtccgcc agattcatcg
      421 tcgcgccccc atctcccgaa gatacgaaaa ccagcgctcg tcgccgatcg cgtccgcgat
      481 ggtaaagatg tccttgtcgc cgcgccccga gagattgatc aggacgatct cgtccggccg
      541 catcgacggg gcctcgcgga tggcgccggc gaaggcgtgg ctgctctcca gcgccgggat
      601 gatcccctcg gtcgcgatga gcagcttcgc cgcggctatc gcctcgtcgt cgcgcgccgc
      661 ggtgaagcgc acgcgtccgg tgtcggccag gtgcgcgagg atgggactca cgccgatgta
      721 gtcgagcccg gcggccaccg agtgcgtttc gcgcatctgt ccctcgtcgt cctgcaggaa
      781 gagcgttttg tagccctgcg ccacgccgat atcgtgcgcc gggatgcggg cggcgtgcct
      841 gccgctctcg atcccatggc cgcccgcctc gacacccacc agctccacct cggggttctt
      901 gatgaacgga acgaacatgc ccaaggcgtt cgaaccgccg cccacgcagg cgtatacgcg
      961 atcgggcagg cgaccggact gcgtgagcat ctgcccgcgg gcctcctgcc cgatgatgga
     1021 ctgaaaatac gaaaccatgt gcgggaaggg atgcgggccg caggcggtcc cgagcacgta
     1081 gtgcgtcgag tccatggact cggaccagtc gcgcaggcag gcgttgatcg cgtccttgag
     1141 cgtggccgac ccgtcctcga tggggacgac tgtcgcgcca agctgctcca tccagaagac
     1201 attgggccgt tgccgccgaa cgtccacggc gcccatgtag atggtgcact cgaagccgaa
     1261 gcgcgccgcc atcgtggccg tcgcgacgcc gtgctgtccc gcgccggtct cggcgatcac
     1321 gcgggtcttg ttcatgcgct tcgcgagcag cccctggccg atcacgttgt tcaccttgtg
     1381 cgcgccggtg tgattgaggt cctcccgctt gcagcagatc gtcgcgccgc cgagtttctt
     1441 tgtaagattg ggaagcggcg taacgggcgt cggccgacag gagtaggtct gcagctcgcg
     1501 gacgtaagct ccccagaatt gatcgtcggc gcgcgcggca tcgaaggccg cgatgagctc
     1561 gtcgagcgtc gcgcgaagga tctcgggcac gaacgctccg ccatactggc catagtaccc
     1621 gggtttcgtg aaatcgaaga gttcttgtgt cattgttccg cctttatgtg agtgtgttca
     1681 gattatgctt cagtatcgcg atcgaaagcc ttctcatctt cattcggtcc ttccggccgg
     1741 gtgcgctctc ggcgccgctg gccacgtcga tcacgcccgg attcacgcgc tcgagggcct
     1801 cggcgatgtt atcgacggtg atgccgccgg cgagcgcgag ccgctcacgc ggaaacgacg
     1861 ccgggatgag gtcccagtcg aaggttttcc cggttcctcc cagcgcgctc ttggaatgcg
     1921 tgtcgataag gagcattgcc gcgccatgca tctgtgcgct gaacggcgtc ccgtcgccga
     1981 tgcggcgcgc gtggatgacc gggacgggaa gtgccgagta gtcggggatg tcgaccggat
     2041 cgtagatttg cagaaagcgc acgccgctgg ttgcgacggc ctcgagtgcg tccgcgagcg
     2101 tcggtcggac cagcagtccc acgcacatcg ccactgacgg caggtgctcg atgatccccg
     2161 ccgcggtttt cggatcgata tatcgcgggc tctcccggaa gaaattgaat ccgagcatgt
     2221 cggccccctc atcgatcgcc atacacgcgt catcgagcga ggttatgccg cagattttaa
     2281 gaagcgtctt catcgttcac ctcgtcgatg atccgccgca ggagcgccgc cggatcggag
     2341 ctccgcatga aggtcttgcc gacgagagcg ccgcgaatgc cgttcgcttt gagtagccgg
     2401 cagtcgtcgg atccggtgat gccgctctcg ccgatcacca gcgcatcggc gggcagcagg
     2461 cggcgcaggg aaaccgatcg gttgatgtcg accgcgaagg tcgccaggtc gcgattgttg
     2521 atgccgacga tgatcgtgtc gcacgacggc agcttgtcga tatcggattc atcggcaagt
     2581 tccagcagca cgtccatgcc gaggccccgg gcgagcgcgg catattcgtc gatctgcgaa
     2641 ctcgtgagca cgcgcgcgat cagcaatatc atgtccgcgc ccgcggcgcg agcctcgtgg
     2701 atctggtacg gatcgatgat gaaatccttc cggagaatgg ggaggcctac cgtttcccgg
     2761 atcgccgaca ggtgatcgag tgagcccatg aagaatttct cgtccgtgag cacggagatc
     2821 gccgacgcgc cgccgtcatg gtacgagcgg gcctggcgga ccggatcgaa atcgtgccgg
     2881 atgatcccct tcgagggcga cgccttcttg atctcgcaga tgaaatgaaa acgatcatcg
     2941 cgcgcgagcg catcgcgaaa gctcacggtc gctcggcgat atcgttcggc gtcgatgagc
     3001 gcggagagcg gggccctgcg tttccgctcg gcaacctcgc cgcgcttgtg ttcgatgatg
     3061 cgatcaagta tcgtcatgcc gttagatccg tagcgagaag tccttgaatt ggctgagctt
     3121 cttgagcgcc gcgccgctgt cgatgctctc ctcggccatg cggatgccgt cctcgatgct
     3181 tgacgctcgc ccggcgacat agaagccgaa ggcggcattg accaccgtca tgtcccgtgc
     3241 ggtggatcgc tcgccctgca gcacttgcat ggtcatgcgg atattgtcgt cgatgcccgc
     3301 gcccttcacc tgcgcgagcg acgcggtggc gtttgcgcgc ggggggcggt actcgtagcg
     3361 ccgcggcgcc atcacccccg gagcggtctc gaagaccgtc gtgtcggcga agatagatat
     3421 ctcgtcaaga ccgtcgttcc cgtggacggc gcacaccttg cgcgatccac gcgcctgcag
     3481 cacggcggcg atcttcccgg ccgtttcgag attgaaggcg cccacgagct gccgcgtcac
     3541 gcgcgccggg ttgagcatgg gccccagcat attgaaaaag gtgcgcagcc ccagcttctt
     3601 tcggtacggg gcgacggcct tcatcgccgg atggaagagc ggcgcgaaaa agaacccgat
     3661 cccgatctcg tcgatgcagc gcttcacttg cgccgagttg aggtcgatct tcacgcccag
     3721 cgcctcaagg agatcggccg acccggtttt gctcgatatc gaccgattgc cgtgcttggc
     3781 caccgtgacg ccgcccgccg cgatcaccag ggccgccgtc gtcgatacgt tgaaggtgaa
     3841 gctcgaatcg ccgcccgttc cgcacacgtc gatcgcgtcc gcgtcggcga ggctcacggc
     3901 gtccatgtgc cgctccatgg tgtccacgaa gccaacgagc tcgtccaccg tctcgccctt
     3961 gatgcgcagg cccacgagaa gcgcgccgat ctccgtctcg ctcgcatcgc cggcgatgat
     4021 cttctcgagg acgcaggagg cctcctcgcg cgcgaggtcc ttgcctgcgg tgattttctc
     4081 caggaaaggc ctgatggcgt tctcgcccgc gggtttcgca attgccggga ccgccgcctt
     4141 cgcgttcgcg gccgcgtgct cggcggtgat cgcgagccag ttcgcgatga tctgcatgcc
     4201 gtgcgtggtg aggatcgatt ccgggtggaa ctgcacgccg aaggtcggat gcgacc
//
LOCUS       DAORHF010000301         4252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22377_length_4252_cov_5.435073, whole genome shotgun sequence.
ACCESSION   DAORHF010000301 DAORHF010000000
VERSION     DAORHF010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22377_length_4252_cov_5.435073"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PKJ16_17130"
     CDS             <1..251
                     /locus_tag="PKJ16_17130"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HNV48769.1"
                     /translation="TVGEADDKPDVMVFYNTGVRLAVTGSPVLDDLRLIASKGARILV
                     CGTCLKFFNLTESLAVGNVSNMYEIKELLFSAGRLVRP"
     gene            248..487
                     /locus_tag="PKJ16_17135"
     CDS             248..487
                     /locus_tag="PKJ16_17135"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNV48770.1"
                     /translation="MTSTEANSILIFHSVHHAIWAESVLRKGGIECKMIPIPRSISSD
                     CGVCVRIASVDESRAAAALSELAEEWRIEPYPARG"
     gene            627..1784
                     /locus_tag="PKJ16_17140"
     CDS             627..1784
                     /locus_tag="PKJ16_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HNV48771.1"
                     /translation="MTPLFQDEFAGGPPAKRSLQYVLGKREKGSPVVGTYCTYAPIEL
                     VRAMGGVPAMLCAFAQGPIEAAETVLPRNLCALIKSSYGFIKTDTCPFFTVSEAVIAE
                     TTCDGKKKMFELIADKRPLHVMDLPQLPDEPGAVANWSAMVVRLKSFLEKTFSRSITD
                     QAVEQAIADTNKRNALVRRFLAYNARRPAVLSWSEIYDVTWLAASASGADLETMMEGV
                     CARLDERIKSGAVIGDAKSPRVLITGCPVGGDATKAFKTIEAAGGVIVAIDNCTGSKL
                     YAEDIDAKSGDPMAALARRYLKIPCSCMTPNTGRLTELDRMIAEFKPDIVVDVVLQAC
                     HGYNIESVKVEEHVKKNHGLPFLKIETDYSSADVEQIRTRVEAALEGTRKK"
     gene            complement(1910..2293)
                     /locus_tag="PKJ16_17145"
     CDS             complement(1910..2293)
                     /locus_tag="PKJ16_17145"
                     /inference="COORDINATES: protein motif:HMM:NF015754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCCL domain-containing protein"
                     /protein_id="HNV48772.1"
                     /translation="MKNVLMILAVVCCAALLASCAQGVAVDCATKMRDVKVEKKDEGA
                     SFLVKCPAACASGSVWGVDTYTTDSSPCVAAVHAGVIKADKGGVVKITVVKGLDSYPG
                     SQRNGVTTSEWKTSWGTTAFTVSAK"
     gene            2394..3299
                     /locus_tag="PKJ16_17150"
     CDS             2394..3299
                     /locus_tag="PKJ16_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HNV48773.1"
                     /translation="MKTRSIKRVFKGKPTIEGAGVHLRRVFGHSEVPWFDPFLLFDDF
                     RSDEPARFRKGFPWHPHRGIETITYVLAGDVEHGDSLGNAGVISSGDVQWMTAGSGII
                     HQEMPKGDGRGRMYGFQLWANLPAAHKMMAPRYRDVRAGDIPEVTLDGGVRVRIICGA
                     VAGARGPVADIVIEPEYLDITVPAGTEYRHPVKAGHTAMAYVIEGKGFFCREKKPFSH
                     DVAGETYFDFAREPYLEDGSLALFDDGDEVLVFTEEEPVRLLFIAGRPIGEPVAWGGP
                     IVMNTREELRIAFEEYENGTFIKSS"
     gene            complement(3408..3992)
                     /locus_tag="PKJ16_17155"
     CDS             complement(3408..3992)
                     /locus_tag="PKJ16_17155"
                     /inference="COORDINATES: protein motif:HMM:NF028235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48774.1"
                     /translation="MKQPVKDKIVFTALVLFYEQGIHHTGINQIIEASDVAKASFYHH
                     FPSKDDLVDECLRQYGAIIADQIRSLVAESGDLDTLIRNWVARIKAGIVDTASYHGCP
                     VANMRFSLMLDSDQNIREKFSAVIDSWADILDAFIETLKKDGRIAHTIVTRQLARRMI
                     HIYQGAVVMWKLTGDTTYFDDLIDIAHELVGPPK"
BASE COUNT          864 a         1197 c         1279 g          912 t
ORIGIN      
        1 atactgtcgg cgaggcggac gacaaaccgg atgtcatggt tttttacaac actggcgtga
       61 ggcttgcagt gacgggctcg cccgtgctcg acgatcttcg cctcatcgcc tcgaaggggg
      121 cgagaatcct ggtgtgcggc acttgcctga agtttttcaa cctgaccgag tcgctcgccg
      181 tggggaacgt ttccaatatg tatgagatca aggaactgct ctttagcgcg ggccgactcg
      241 ttcggccatg acctcgacgg aggcgaattc gatactcatc ttccactcgg tgcaccatgc
      301 catctgggcc gaaagcgtcc ttcgaaaggg cggcatcgag tgtaagatga tccccatacc
      361 ccggtcgata agctcggatt gcggcgtgtg cgtccgcatc gcgtccgtgg atgagtcacg
      421 cgctgccgcg gcgctctccg agcttgccga ggagtggcgc atcgagccgt atccagcgcg
      481 cggataggtc cggcgtgggg gcttttttga atccaataat cagaaatatt aatactgtat
      541 atcgataaat atgattgacc attgtggctg gtcgggatcg acttgtgccc gcgcaccatc
      601 ttccatatga ataccgaggt acagccatga cgccattgtt tcaggatgaa ttcgccggag
      661 gccctcccgc caaacgctcg cttcagtatg ttctgggcaa gcgcgagaag ggaagccccg
      721 tcgtcggcac ctattgcacc tacgctccca tagaactggt ccgcgccatg ggcggggttc
      781 ccgcgatgct gtgcgcattt gcacaggggc ccatcgaggc ggcggaaacc gttctcccgc
      841 gaaacctgtg cgcgctcatc aagtcgagct acgggtttat aaaaaccgac acctgtccct
      901 tctttaccgt ttccgaggcc gttatcgcgg aaacgacctg cgacgggaaa aagaagatgt
      961 tcgaactcat cgcggacaaa cggcccctgc acgtcatgga tcttccgcag ctccccgacg
     1021 agccgggcgc ggtggccaac tggagcgcca tggtggtccg cctgaaatca tttctcgaga
     1081 aaaccttttc gcgctccatc accgatcagg ccgtcgagca ggccatcgcc gatacgaaca
     1141 agcgaaatgc gctggtgcgc cgctttcttg cgtataacgc ccgccgtccc gcggtcctca
     1201 gctggagcga gatatacgat gtcacctggc tggcggcctc ggcctcgggg gcggacctcg
     1261 aaaccatgat ggaaggcgta tgtgcgcgtc ttgatgagcg gatcaagtcg ggtgcggtta
     1321 tcggcgatgc gaaatcgccg cgcgtgctga taacgggatg ccccgtcggc ggcgacgcga
     1381 cgaaggcgtt caagaccatc gaggccgccg gcggcgtcat cgtcgccatc gacaattgca
     1441 cgggaagcaa actgtatgcg gaagatatcg atgcgaaaag cggcgatccc atggcggcgc
     1501 tcgcgcgtcg ctacctgaag atcccgtgct cgtgcatgac gcccaataca ggacggctta
     1561 cggaactcga tcgcatgatc gcggaattca aacccgatat cgtggtcgac gtcgtacttc
     1621 aggcatgtca cggttacaat atcgagtcgg tgaaggtcga ggagcacgtt aaaaaaaacc
     1681 acggactgcc gttcctgaaa atcgagacgg attattcaag cgccgatgtg gaacaaatcc
     1741 gcaccagggt cgaggccgcg ctggagggca ctcgaaagaa atagcccgta tgagaattca
     1801 catcgcggtt cgagccgtcg catgcttcaa gcagggtgtg cgatgataac gagaaaagcg
     1861 ggcgaatatg cgcccgcttt tctcgttgaa tgttctgagg agtgtacggc tatttcgcgg
     1921 aaacggtaaa cgccgtggtt ccccaggagg tcttccattc gctggtggtc acgccgttgc
     1981 gctggctgcc ggggtagctg tcgagccctt tcacgacggt gatcttcacg acgccgccct
     2041 tgtcggcctt gatgacgccg gcatggaccg ccgccacgca gggagaggag tcggtggtgt
     2101 aggtatccac gccccacacg ctgccgctgg cgcatgccgc cggacacttg accaggaagc
     2161 tcgcgccctc gtccttcttc tcgaccttca cgtcgcgcat cttggtggcg caatcgacgg
     2221 caacgccctg ggcgcagctt gccaggagcg cggcgcagca tacaaccgca agaatcatca
     2281 gtacgttttt cattggtaac ctcgctattt aattgatttc tgccatcgat catgctattg
     2341 tttgggtaca gtgtcaatga ttattcgatt tttttccagc gcagggggaa gcggtgaaaa
     2401 cgcgttcaat caagcgggtg ttcaagggca aaccgacaat cgagggggcc ggtgtccatc
     2461 ttcgacgcgt gttcggtcac agcgaggtcc cctggttcga tccgttttta ttgttcgatg
     2521 attttcgctc tgatgagccg gcccggttcc gcaagggatt cccctggcat ccgcatcgcg
     2581 gcatcgaaac catcacctac gtgctggccg gagatgttga gcatggcgac agcctgggca
     2641 atgccggcgt catctcctcg ggtgacgtgc agtggatgac cgccggaagc ggcatcatcc
     2701 atcaggagat gccgaagggc gacgggcggg ggcggatgta cggattccag ctctgggcga
     2761 accttcccgc cgcgcacaag atgatggcgc cgcgctatcg cgacgtgcgc gccggcgaca
     2821 ttcccgaggt cacgctcgac ggcggcgtgc gcgtgcgtat catctgcggc gcggtcgcgg
     2881 gcgctcgcgg gcccgttgcg gacatcgtca tcgaacccga atacctcgac atcaccgtcc
     2941 cggcggggac cgagtaccgg caccccgtga aggcggggca caccgcgatg gcgtacgtta
     3001 ttgaggggaa ggggtttttc tgccgggaaa aaaagccgtt ctcgcatgat gtggcgggcg
     3061 agacctattt cgattttgcg cgcgagccct acctcgaaga cggatcgctc gcgctgttcg
     3121 acgacggcga cgaggtcctc gtcttcaccg aggaggagcc ggtgcggctg ctgttcatcg
     3181 cggggcgccc catcggcgag ccggtcgcct ggggcggtcc catcgtcatg aacacgcgcg
     3241 aggagctgcg gatcgccttc gaggaatacg agaacgggac ctttatcaag tcctcatgag
     3301 gtcgtattcg cgatacacat gatcacgttg tcgctggagc ggtagtcgca caggcggccc
     3361 ttgacgaacg tgatggcggt tcagaacagg gaatcacaat caagatttca tttcggcgga
     3421 cccaccaact cgtgggcgat gtcgatgaga tcgtcgaaat aggtcgtgtc tccggtgagt
     3481 ttccacatga ccacggcgcc ctggtagatg tggatcatcc gccgtgcgag ttgccgcgtg
     3541 acgatggtgt gcgcgatgcg gccatccttc tttagcgtct cgatgaaggc gtccaggata
     3601 tccgcccagc tgtcgatgac cgcggaaaac ttttcccgta tgttctggtc cgaatcaagc
     3661 atgagactga agcgcatatt cgcaacggga catccgtggt acgatgcggt gtcgacgatg
     3721 cccgccttga tccgtgcgac ccagttgcgt atcagtgtgt cgagatcgcc gctttccgcg
     3781 accagcgagc ggatctggtc cgcgatgatg gcgccgtact gccgcaggca ctcgtccacc
     3841 aggtcgtcct ttgacgggaa gtgatgatag aaactggcct tggcgacatc ggaggcctcg
     3901 atgatctggt tgattccggt atgatgaatt ccctgctcgt agaacagcac caatgccgtg
     3961 aaaacgattt tgtcttttac cggttgtttc ataggccctt ttatattaat tcgataacaa
     4021 attatgcttg acagtagaca gacatgtctg tatggtgtca agcatattat tgaatacttt
     4081 tttaaggggc cacaatgcaa atccaggttc caaacgttcc catagcattt tttgatttgg
     4141 atgaaaccat catcaacgag gacgccaatt cgctctggtt gaaatggcgc gtcccgagaa
     4201 gcgtgctcag tcttccggag ctgtgtatcg gattgtataa ccggcactat ta
//
LOCUS       DAORHF010000302         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22437_length_4242_cov_5.498448, whole genome shotgun sequence.
ACCESSION   DAORHF010000302 DAORHF010000000
VERSION     DAORHF010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22437_length_4242_cov_5.498448"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="PKJ16_17160"
     CDS             complement(<1..1671)
                     /locus_tag="PKJ16_17160"
                     /inference="COORDINATES: protein motif:HMM:NF017437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HNV48775.1"
                     /translation="MYEFTKRSRKVLEVYAQSEGRRLNSDSLGPEHILLALIKDNDSI
                     AARILKNLGVNFDVLRRNIELAAKKSGTTIILGNVPISTRFNRIIEIAKDESRKLKNS
                     YIGTEHLLLSIFRESTCSGLDNLIKSGVDYAVVKSEILKILGLQQNAGGEKQSSEKGK
                     TPSLNEFSEDLTHMAAQDKLDPVIGRHAEIERVIRILSRKRKNNPVLIGEPGVGKTAI
                     VEGLAQRIVAKQVPEPLQNRRVLALDMAAIVAGTKYRGEFEERLKRVIKEIRASGNVI
                     VFIDELHTIIGAGAAEGAIDAANILKPTLARGELQCIGATTLNEYKLYIEKDAALERR
                     FQSVLVEEPDTEQTLEILRGLKQRYESHHKVTYSDEALEAAVYLSDRYINDRYLPDKA
                     IDIIDETGSKARLEHCDRPTDIVALEGEIDEINERKNDLVRSQEYEQAAALRDLINEK
                     KGQLAAKVNDWQQKINEYRIIVDDGQIAAVVAQWTGIPVEKLTESETEKLLRIEEELH
                     HRVIGQDEAIGVVSRAIRRSRTGLRSPDRPIGSFIFIGPTGVGKTELAK"
     gene            complement(1725..2759)
                     /locus_tag="PKJ16_17165"
     CDS             complement(1725..2759)
                     /locus_tag="PKJ16_17165"
                     /inference="COORDINATES: protein motif:HMM:NF012443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48776.1"
                     /translation="MFDYLLERPGFWSVGRSSDVVISTRARLARNMPSIPFPGSMSEH
                     ETGLLRSVAHRFFEQSTLANLSYIELQGLDTVDRRFLRERNIITYEMEAGDSSFVIFD
                     NDERFEIFVNEEDHFRVQAMRPGLQAHECYEEANRVEDELNRFVSFAFSEELGFLTAS
                     PANLGTGLRASSMVHLPSLTALKRMTDVTAAIAERGFLIRSTLGDEQKSVGGMYVVCN
                     RASLGVSEVDIVEALDTVVAMIVEEENRARDDWCANYRQQLEDRVWRSYGLLRYSRSI
                     GFLEAIANLANIRLGTILSIVKDIDLGAIHELMVRIQYAHLQKHFGAAFTGEGECDAF
                     RAQYIRDQFN"
     gene            complement(2761..3303)
                     /locus_tag="PKJ16_17170"
     CDS             complement(2761..3303)
                     /locus_tag="PKJ16_17170"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HNV48777.1"
                     /translation="MLCEKCKTRDATVHLTEIMKNVKSEIHLCEKCAKEFGLNTKFSK
                     FSLTVPDMLSFLDVHEVNPSDRTAVCMSCGSSYIDIRKRGRLGCPDCYRYLGAELESI
                     ILNVHGRNRHVGKIPAYYVEMKSFSKPLIDSDLPPAGEMSDLDELRRQLDDAVMNERY
                     EEAALLRDRIRGLQAKAHGA"
     gene            complement(3441..3698)
                     /locus_tag="PKJ16_17175"
     CDS             complement(3441..3698)
                     /locus_tag="PKJ16_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48778.1"
                     /translation="MIDQHDALTCHCRMLGHEVPFSYCRKLQSGIPCSRIADCWFEYI
                     GIEDFLKSQYSEDELKKIFSPPRPKMATIIDIVNRLNQNNS"
     gene            complement(3797..>4242)
                     /locus_tag="PKJ16_17180"
     CDS             complement(3797..>4242)
                     /locus_tag="PKJ16_17180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948697.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HNV48779.1"
                     /translation="TPLLPMHTLGHAFIPEPIHAGGLRYHGMAPLVSQAVVEGLLSPR
                     SYRQNDCYEAALLFARTEGFIPAPETSHAIKCAIDEAVKAKEEGKEKVIIFNWSGHGL
                     MDLVGYDKYMSGQLVDHELSDADMQKSLKAIANLPKAKAVKSGRW"
BASE COUNT          819 a         1298 c         1199 g          926 t
ORIGIN      
        1 cttcgcgagc tcggtcttcc ccacgccggt gggcccgatg aagatgaacg acccgatggg
       61 ccggtcgggg cttcgcaggc cggtgcgtga tctcctgatg gcccgactga ccacgccgat
      121 ggcctcgtcc tgcccgatca cccgatggtg cagctcctcc tcgatgcgaa ggagcttctc
      181 cgtctccgat tcggtgagct tctccaccgg tatgcccgtc cactgcgcca ccaccgcggc
      241 gatctggccg tcgtcgacga tgatccggta ttcgttgatc ttctgttgcc agtcgttcac
      301 ctttgcggcg agctggccct tcttctcgtt gataagatcc cgcaacgcgg cggcctgctc
      361 gtattcctgc gaacggacca aatcgttctt tcgctcgttg atctcgtcga tctcgccctc
      421 gagcgcgacg atgtcggtgg gccggtcgca gtgctccagc cgcgccttcg atcccgtctc
      481 gtcgatgatg tcgatggcct tgtccggcag gtagcggtcg ttgatgtagc ggtccgagag
      541 atataccgcg gcctcgagcg cctcgtcgga ataggtcacc ttgtgatggc tctcgtagcg
      601 ttgtttgagc ccgcgcagaa tttcgagggt ctgttcggtg tcgggctcct ccaccaaaac
      661 gctttggaaa cgccgctcga gcgcagcgtc cttttcgatg tagagcttgt attcgttgag
      721 cgtggtcgcg ccgatgcact gcaattcgcc cctcgcgagc gtcggcttga ggatgttcgc
      781 cgcgtcgatc gcgccctcgg ccgcccccgc accgatgatg gtatgcagct cgtcgatgaa
      841 cacgatcacg ttgccgctgg cgcgtatctc cttgatgacc cgcttgaggc gctcctcgaa
      901 ctcgccgcgg tacttggtcc cggcgacgat cgcagccatg tccagcgcga ggacccgtcg
      961 gttctgcagc ggctccggca cctgcttggc gacgatccgc tgcgcgagac cctccacgat
     1021 ggcggtttta ccgacgccgg gctccccgat gagcaccggg ttgttcttcc gcttgcggga
     1081 gaggatgcgg atcacgcgct cgatctcggc atgcctgccg atcacgggat cgagcttgtc
     1141 ctgcgcggcc atgtgcgtaa ggtcttcgga aaactcattg agactcggcg tcttgccctt
     1201 ttcggacgac tgcttctccc cgccggcgtt ctgctggagc cccagaatct tcagtatttc
     1261 gcttttcacg acggcgtaat cgacgcccga tttgatgaga ttgtcgagac cggagcaggt
     1321 gctctcccga aagatcgaca ggagcaggtg ctcggtgccg atgtagctgt ttttcagctt
     1381 tcgggactcg tccttggcga tttcgataat gcggttgaag cgggtgctga tgggcacatt
     1441 gcccaggatg atcgtcgtgc cgctcttctt cgcggccagc tcgatatttc gacgtaacac
     1501 gtcgaaattg acgccgaggt tcttcagaat gcgcgcggcg atagaatcgt tgtctttgat
     1561 aagcgcgagg aggatgtgtt cgggccccag cgaatcggaa ttgaggcgcc gcccctccga
     1621 ctgcgcatag acctcgagga cctttcgtga ccgtttagtg aactcgtaca tgatcgctcg
     1681 ccgtatgccc tttccgtacc actatagccc gccatgcggt cctgtcaatt aaattgatcg
     1741 cgaatgtact gcgcgcggaa cgcgtcgcat tcgccctcgc cggtaaacgc cgcgccgaag
     1801 tgtttctgca aatgggcgta ttggatgcgg accatgagct cgtgaatcgc cccgaggtca
     1861 atatccttga cgatggaaag gatcgtcccc aaccgtatgt tcgcaagatt cgcgatcgcc
     1921 tccagaaagc cgatgctgcg ggaataccgc aacaatccat aggagcgcca cacgcggtcc
     1981 tcgagctgct gccggtagtt cgcgcaccaa tcgtcgcgcg cgcggttctc ctcctcgacg
     2041 atcatcgcca cgacggtgtc gagcgcctcg acgatgtcca cctcggacac gcccagcgag
     2101 gcgcggttgc atacaacgta catgccgccc acggacttct gttcgtcgcc gagcgtgctt
     2161 cggatcagga agccgcgctc cgcaattgcc gccgtgacgt ccgtcatgcg cttgagcgcc
     2221 gtgagcgacg gcaggtgcac catcgacgag gcgcgcaggc ccgtcccgag gttcgccggc
     2281 gaggccgtga ggaagccaag ctcctccgaa aaggcgaacg atacgaagcg gttcagctcg
     2341 tcctcgactc tgttcgcctc ctcgtagcac tcatgcgcct ggagtcccgg gcgcatcgcc
     2401 tggacgcgaa aatggtcctc ctcgttcacg aatatctcga accgctcgtc attgtcgaaa
     2461 atgacgaaac tcgagtcgcc ggcctccatc tcataggtga taatgttccg ctcgcgcaaa
     2521 aaacgcctgt ccacggtgtc cagtccctgc agttcaatat agctgagatt ggcgagggta
     2581 gactgctcga aaaagcggtg ggccaccgag cgcaagagcc ccgtctcatg ttcgctcatg
     2641 ctgccgggaa atggaatgct cggcatgttg cgcgcaagcc gtgcgcgcgt ggatatgaca
     2701 acatccgatg atcgcccgac ggaccagaag ccgggccgtt cgagaagata gtcgaacacg
     2761 ctaggcacca tgcgccttgg cctggagccc cctgatccgg tcgcgcagca acgccgcctc
     2821 ctcgtaccgc tcgttcatga ccgcatcgtc gagctggcgg cgcaattcgt cgagatcgct
     2881 catttcgccg gccggcggca ggtcgctgtc gatgagcggt ttggagaacg atttcatctc
     2941 gacataatac gcgggtattt tccccacgtg acggttgcgc ccatgaacat tcagtatgat
     3001 cgactcgagc tcggcgccga gatatcgata gcaatcggga cacccaagcc gtccccgctt
     3061 tcggatatcg atataggacg aaccgcagct catgcacacc gcggtgcggt cggagggatt
     3121 gacctcgtgc acgtcaagga aggaaagcat atcgggaacc gtgagcgaaa atttggaaaa
     3181 tttcgtgttg agaccgaact cctttgcgca tttttcgcac agatgtatct ccgacttcac
     3241 atttttcatg atttcggtga gatgcaccgt cgcatcgcgc gttttgcatt tctcacacag
     3301 catcactcga cccgactccg ctcaaacaaa ttatgagaca taatatatca tcacaatgat
     3361 cacggcaatc aaaaaaacaa tgaaaagtta acaaacatat aattcctgtt cattaatata
     3421 gcggcaaaga cgatggggaa tcaagaatta ttttgattta acctgttcac aatatcgata
     3481 attgtagcca tttttggccg cggaggagaa aaaatctttt tgagttcatc ttcggaatac
     3541 tggcttttca ggaaatcctc gatcccgata tattcgaacc agcagtccgc gatgcgtgag
     3601 cagggaatgc cggactgcag cttacgacaa tacgaaaacg gaacctcatg gcccagcatt
     3661 cgacaatggc aggtgagcgc atcatgttga tcaatcatcg cattcgtcct caaatgctcc
     3721 cggaggaaca ccggcgcgac cgaaatgaat cggctgcgcc ggcatccatg tccgatgagc
     3781 ccgcagtacg atactcttac cacctgccgc ttttcacggc cttcgcctta ggcagattcg
     3841 caatcgcttt cagcgatttc tgcatgtccg cgtcggagag ctcgtgatcg acgagctggc
     3901 cgctcatgta cttatcgtag ccgaccaagt ccatcagccc gtgcccgctc cagttgaaga
     3961 tgatgacctt ctccttgccc tcctccttcg ccttcacggc ctcgtcgatc gcgcacttga
     4021 tcgcatggct ggtctccggg gccggaatga atccctcggt gcgcgcgaac aggagcgccg
     4081 cctcgtagca gtcgttctga cggtaggagc gcggcgagag cagcccctca accaccgcct
     4141 ggctcaccag cggcgccatg ccatgatagc gcaggccgcc ggcgtgaatc ggctcgggga
     4201 tgaacgcatg ccccagggtg tgcatcggca acaggggcgt cg
//
LOCUS       DAORHF010000303         4240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22458_length_4240_cov_5.494146, whole genome shotgun sequence.
ACCESSION   DAORHF010000303 DAORHF010000000
VERSION     DAORHF010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22458_length_4240_cov_5.494146"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PKJ16_17185"
     CDS             complement(<1..246)
                     /locus_tag="PKJ16_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48780.1"
                     /translation="MRISSGIAEITSLSTSAGPRLEKTYLWPVYNNGTVNKIHGVTRR
                     TDSSAVYTKPLGDDHNKLLRLSMTAAESEYTSKGNVIS"
     gene            complement(399..818)
                     /gene="nikR"
                     /locus_tag="PKJ16_17190"
     CDS             complement(399..818)
                     /gene="nikR"
                     /locus_tag="PKJ16_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HNV48781.1"
                     /translation="MSNLIRFGVSIDEKLLTRFDATIGEKGYVNRSEAIRDLIRDMLV
                     AEETSDPSAEAMGTLTLVYSHEVREIADKLNDIQHNYYGNIVSAVHVHLDEHNCLEVL
                     ILRGKSKTVKEIADRLLSIKNVRHGKLTITSAGSQHA"
     gene            complement(921..2906)
                     /locus_tag="PKJ16_17195"
     CDS             complement(921..2906)
                     /locus_tag="PKJ16_17195"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HNV48782.1"
                     /translation="MSASSARSPLFSAVIFLVLACSPGPARAAGIELDVIPASGGYAH
                     SANGGGSWTALPDGLPAGAVAERLYRDAAGNRWMATRGHGLFLLPAGAREWRARNSDA
                     FRYRGRGPAGPMRSVSAFAVDRENPNELALATKHTLFASSDGGATWRLIPLRGGLSSK
                     TYITSLHRALGHTLAGTSFEGLYWAHGSSFRKSSSGIPYEAYSGQHWFYDEVSAIEVD
                     PSNRNRLFAGLSSGNGVYESTNGGASWTSLALPLARESHFFVWDVKIAENRLYAATDG
                     GIFEFDLAARRWSASKLADVIHAIPKKYNAAAVLITLSKEKVSLFHRTVAQVNRKQEP
                     PMKAAAGRDALYVGYYPFVKNFSSVMKTLALTGMNALVIDMKDDLGSVFYPVTNATAN
                     EIKAVKKAPDLPRLIKEAKARGVYLIARIVVFKDWHLYRAYRSKYAIWNYRTGAPWTG
                     NPGEYWVDPHAEFVHDYNIAIAKEIAAAGFDEIQFDYIRFPLDGPVHLCSFRHRTDAD
                     TYKSEAINDFLMKARAAISAPISVDIYGLNAWYRFGNRIGQNIEELAWIVDAICPMAY
                     PSHFGGRFFGRYGHQERPYHIIRETGRRSIAFVGPQAYIRPYLQAFNLLSPTWGPEYI
                     ASQVDAARASGCSGFALWNANAEYSVARRALEKKSGD"
     gene            complement(2893..>4240)
                     /locus_tag="PKJ16_17200"
     CDS             complement(2893..>4240)
                     /locus_tag="PKJ16_17200"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HNV48783.1"
                     /translation="ALLFNEIVRDRKIQLYIDERVDDHTIRIKSAYRGHIKQALIRIG
                     FPVEDLAGYDDGDPCLFDLREIRAGGAPFAVRDYQQSAINAFYRGGGPEGGSGVIVLP
                     CGAGKTIVGIGIMALLKTETLIMVTNTVALRQWRDELTDKTGIDPALIGEYSGERKEI
                     KPVTIATYNIITYRKKKHEDFLHFNIFMSKNWGLIIYDEVHLLPAPVFRITSEIQSKR
                     RLGLTATLVREDGLETDVFSLIGPKKFDMPWKILEKTNWIATAICTEIRVELPDDLRM
                     QYSLASDKEKFRVASENERKTAIVRRILAHHEGANVLIIGQYLTQLGDLAAALSFPII
                     TGSTPIAERERLYAAFKKGDIRNLIVSKVANFAIDLPDANVAIQISGTFGSRQEEAQR
                     LGRILRPKADDNRAYFYTIITTNSAEEKFAHNRQLFLTEQGYSYIILNEDMFNERF"
BASE COUNT          695 a         1258 c         1435 g          852 t
ORIGIN      
        1 gctgatcacg ttgcctttcg aggtgtactc gctttcggcg gcggtcatcg agaggcgcag
       61 gagtttgttg tgatcgtcgc ccagcggctt ggtgtacacg gcgctggagt cggtgcgccg
      121 cgtgaccccg tggatcttgt tcacggtgcc gttgttgtac accggccaca gataggtctt
      181 ctccaggcgc ggtccggcgc tggtggacag actcgtgatc tccgctatgc cgctgcttat
      241 cctcataccg gtcatatcgg cagatggaat cgttttcttt accggttttc ccttcgttgg
      301 gttgcggggt tggacacatg ttctcccgtc gcaatgcgga gataaagggg tttccctgcg
      361 gtagatgcga gcgaagacgg tatcgccccc cgacgtttct atgcgtgctg gcttcccgcc
      421 gaggttatgg tgagcttgcc gtgtcgcacg ttttttatgg acaggagccg gtcggcgatt
      481 tccttcaccg tcttgctctt cccgcgcagg atgagcacct ccaggcagtt gtgctcgtcg
      541 aggtgcacgt gcacggcgga gacgatgttg ccgtagtagt tgtgctggat gtcgttgagc
      601 ttgtcggcga tctcgcgcac ctcgtggctg tagacgagcg tgagggtgcc catcgcctcg
      661 gcgctgggat cggaggtctc ctccgcgacc agcatgtcgc gaatgaggtc gcggatcgcc
      721 tccgagcggt tcacatagcc cttctcgccg atcgtcgcgt cgaagcgcgt gaggagtttt
      781 tcgtcgatgg aaacgccgaa gcggatcaga ttgctcatgg ggtgcctcgc gaagtgtggt
      841 ccgtgtgttg caaacctgtg gatatacggt gattcgggaa gcgcaagaaa aaaacggatt
      901 ccatcgacag tcccggccgg ctagtccccg ctttttttct ccagcgcccg gcgcgcgacg
      961 gagtactccg cattggcgtt ccacagcgca aacccgctgc agccgctcgc gcgcgcggca
     1021 tcgacctggc tggcgatgta ctccggcccc caggtgggcg agagcagatt gaaggcctgc
     1081 aggtaggggc gtatgtaggc ctgcgggccg acgaaggcga tggaacgcct cccggtctcg
     1141 cggatgatat gataggggcg ctcctggtgc ccgtagcgcc cgaagaagcg gccgccgaaa
     1201 tgcgacgggt aggccatggg gcagatcgcg tccacgatcc acgcaagctc ctcgatgttc
     1261 tggccgatgc ggtttccgaa gcggtaccat gcgttcaggc cgtagatgtc caccgagatg
     1321 ggggccgaga tcgccgcgcg cgccttcatc agaaaatcgt tgatcgcttc ggatttgtag
     1381 gtgtcggcgt ccgtccggtg ccggaacgaa cacaggtgca ccgggccgtc cagcgggaag
     1441 cggatgtagt cgaactggat ctcgtcgaag cccgccgccg cgatctcttt ggcaatggcg
     1501 atgttgtagt cgtggacgaa ctctgcgtgg ggatcgaccc agtattcgcc gggattgccg
     1561 gtccagggcg ccccggtgcg atagttccag atcgcgtatt tcgaccgata cgcccggtac
     1621 aggtgccagt ccttgaagac cacgatgcgc gcgatgagat agacgccacg cgccttcgcc
     1681 tcctttataa gccgcgggag gtccggggcc ttcttgaccg ctttgatctc gttggccgtc
     1741 gcattcgtca cggggtaaaa aacgcttccc aggtcgtcct tcatgtcgat gaccagcgcg
     1801 ttcatgccgg tgagcgcgag cgtcttcatg accgacgaaa agttcttcac gaacgggtag
     1861 taccccacgt agagcgcgtc ccgtccggcg gccgccttca tcggcggctc ctgcttccgg
     1921 ttcacctgcg cgacggtgcg atgaaagagc gacaccttct ctttggagag tgtgatgagg
     1981 accgcggccg cattatactt tttcggtatc gcgtgtatca catcggcgag tttcgatgcg
     2041 gaccaccggc gggcggcgag gtcgaattcg aagatgccgc cgtcggtcgc cgcatagagc
     2101 cggttctccg cgattttcac gtcccagacg aagaagtgtg attcgcgcgc gagcggaagc
     2161 gcaaggctcg tccaggacgc gccgccgttg gtcgattcgt acacgccgtt tccggaggaa
     2221 agtcccgcga acaggcggtt tcggttcgag ggatcgacct cgatcgcgct cacctcgtcg
     2281 tagaaccagt gctggccgga atacgcctcg tagggaatgc cggatgatga tttgcggaat
     2341 gaagacccat gcgcccagta cagcccctcg aaggaggtcc cggcaagggt atgaccgagc
     2401 gcacggtgca gggacgtgat ataggtcttg gacgaaaggc cgccccgaag ggggatcagc
     2461 cgccaggtgg cgccgccgtc cgaagacgca aagagcgtgt gttttgtcgc aagcgcaagc
     2521 tcgttcgggt tctcccgatc gacggcgaag gccgagaccg atcgcatcgg gccggcgggg
     2581 ccccttccgc gataccggaa ggcgtcgctg tttcgcgccc gccactccct cgcgccggcg
     2641 ggcagaagga agagaccgtg cccccgggtc gccatccagc ggtttcccgc cgcgtcccgg
     2701 tacaggcgct cggcgacggc cccggcgggc agcccgtccg ggagggccgt ccagcttccc
     2761 ccgccattgg cggaatgcgc gtacccgccc gacgccggaa taacgtccag ttcgatcccc
     2821 gcggcgcgcg cgggcccggg cgagcaggcg agcacaagga agatgacggc ggagaaaagc
     2881 ggcgagcgcg cgctagaagc gctcattgaa catgtcctcg ttcaggatga tgtagctgta
     2941 gccctgctcc gtgaggaaga gctggcggtt gtgcgcgaat ttctcctcgg cggagttggt
     3001 ggtgatgatg gtgtagaaat acgcgcggtt gtcgtcggcc ttgggccgga ggatccgtcc
     3061 caggcgctgg gcctcctcct ggcgcgagcc gaaggtcccg gagatctgga tcgccacgtt
     3121 cgcgtccggc aggtcgatcg cgaaattcgc caccttggag acgatgaggt tgcggatgtc
     3181 gcccttcttg aaggcggcgt acaggcgctc gcgctccgcg atgggcgtgc tcccggtgat
     3241 gatgggaaac gagagcgccg cggcgagatc gcccagctgc gtgaggtact ggccgatgat
     3301 gaggacgttt gcgccctcgt ggtgcgctag gatgcggcgc acgatggcgg tcttccgctc
     3361 gttctcggag gccacgcgga acttctcctt gtcggaggcg agcgagtact gcatgcgcag
     3421 gtcgtcgggg agctccacgc ggatctcggt gcagatggcc gtcgcgatcc agttcgtctt
     3481 ctcgaggatc ttccacggca tgtcgaactt cttggggccg atgaggctga agacgtcggt
     3541 ctccaggccg tcctcgcgga ccagcgtcgc ggtgaggccc aggcggcgct tggactggat
     3601 ctctgacgtt atgcgaaaaa ccggcgcggg aagcaggtgc acctcgtcgt agatgatcag
     3661 cccccagttc ttggacatga agatgttgaa gtgcaggaag tcctcgtgct tctttttccg
     3721 gtaggtgatg atgttgtagg tcgcgatggt gaccggcttg atctccttgc gctcgcccga
     3781 gtattcgccg atgagcgccg gatcgatgcc ggtcttgtcc gtgagctcgt cgcgccactg
     3841 gcgcagcgcc acggtgttgg tgaccatgat gagcgtttcg gtcttcagca gcgccatgat
     3901 gccgatgccc acgatggtct tgcccgcgcc gcagggcagg acgatcacgc cgcttccgcc
     3961 ctcgggcccg ccgccgcggt agaaggcgtt gatggcgctt tgctggtagt cgcgcacggc
     4021 gaacggcgcg cccccggcgc ggatctcgcg cagatcgaag aggcagggat cgccgtcgtc
     4081 gtagccggcc aggtcctcca ccgggaaccc gatccggatg agcgcctgct tgatgtgccc
     4141 ccggtaggcg gatttgatgc ggatggtgtg gtcgtccacg cgctcgtcga tgtagagctg
     4201 gatcttccgg tcgcggacga tctcgttgaa cagcagcgcg
//
LOCUS       DAORHF010000304         4230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22547_length_4230_cov_4.412216, whole genome shotgun sequence.
ACCESSION   DAORHF010000304 DAORHF010000000
VERSION     DAORHF010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22547_length_4230_cov_4.412216"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PKJ16_17205"
     CDS             complement(<1..406)
                     /locus_tag="PKJ16_17205"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014002070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HNV48784.1"
                     /translation="MILFIDNYDSFTYNLVDYFGQLNPNLMVVRNDAITVDEVREMAP
                     TGIVISPGPGSPVDAGVSCNIIREFHTTVPILGVCLGHQCIGEVFGGRVKRAPEPVHG
                     KTAEIYHKNDRLFGSVPLPFSATRYHSLIIERE"
     gene            complement(387..1865)
                     /gene="trpE"
                     /locus_tag="PKJ16_17210"
     CDS             complement(387..1865)
                     /gene="trpE"
                     /locus_tag="PKJ16_17210"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HNV48785.1"
                     /translation="MTQEQFEALAKPGTIVPVFRKFNSDFITPVMAYLKIREAGTASF
                     LLESVQKGENIGRYSFIGHSPFRTIKSTGGKTVIGDLRANRTLDENFFDVLKRELQNY
                     RSVPVEGISRFTCGAVGFIGYDMIRQIERIPESRRTPVDCDDASLAFYDNLIAIDHLK
                     NEIILIANVFVDDDSHVPTLYRDAGHRLDTIIGKLNAPVTPKTEFHADEASFAANFTQ
                     EEFERAVVRAKEHIFAGDIFQVVLSQRFTIDYTGDVFQAYRSLRGINPSPYLFYLDFV
                     DYQLVGSSPEPLIRLENNELEIIPIAGTRPRGATIEEDNRHAESLLADPKELAEHIML
                     VDLARNDMGRVSRHGSVRVLDFETVEKYSHVMHIISRVRGELKPHLTAVDAFRASFPA
                     GTVSGAPKIRAMEIINDIEPDRRGFYAGSIGYFDYRNNMDMCITIRSMLAKDGKLHLQ
                     AGAGIVADSVPASEYQETLNKGMALRNAIKQASGGINDIIYR"
     gene            2401..2781
                     /locus_tag="PKJ16_17215"
     CDS             2401..2781
                     /locus_tag="PKJ16_17215"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV48786.1"
                     /translation="MLDTCTLLWLASRQEDLSAAARAAIRDSSGGLYVSAISAFEIAI
                     KSKKGALALPMPADAWFTRALSHHGIAEISVDARLFGEAVRLPDHHNDPADRIIIATA
                     LAFSMRIVTPDPLFGRYSGVITVW"
     gene            2802..3650
                     /locus_tag="PKJ16_17220"
     CDS             2802..3650
                     /locus_tag="PKJ16_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV48787.1"
                     /translation="MPEVLTQNIGDADIQYLHYPGDGPVVIMLHATGFLPWMWHPIAR
                     ELSRGYRVIAPYFCDHRHAEPEEGVEWMLLADDLCRLCMGLGIERPYLVGHSMGATVM
                     TLAEAIHGPTARALVLIEPIFLPGEIYSMGLTIDQHPLASKSIKRRNQWDGPDEARDY
                     LRSKALFKNWDDEMLDLYVQYGMVPGETGGLNLACHPRREAALFMGSQKHNPWPLLPD
                     VSCPALVVEGGESENRHFIDLKKAASLIPEGRYHLVAGAGHLVPMERPGEVLSFVQEF
                     FESVPR"
     gene            complement(3751..>4230)
                     /locus_tag="PKJ16_17225"
     CDS             complement(3751..>4230)
                     /locus_tag="PKJ16_17225"
                     /inference="COORDINATES: protein motif:HMM:NF024591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48788.1"
                     /translation="EVHVRSPYVMLGYWNDPQATAAALKPGGWLAMGDVARIEDGLLY
                     INARARDLLLVSAENVSPTEVEHCLDAHPKVLEACAIGVPHATRGESIKAFVVLKPGE
                     TATEDEIIGFCKNKLAPYKLPTEVEFRDDLPKSTVGKILRKELRAEELEQRKKEKAS"
BASE COUNT          787 a         1247 c         1321 g          875 t
ORIGIN      
        1 cctcccgctc gatgatgagc gagtggtagc gcgtcgccga gaagggaagg gggacggagc
       61 cgaagagccg gtcgttcttg tggtagatct cggccgtctt cccgtgcacg ggctccgggg
      121 cgcgcttcac gcggccgccg aacacctcgc cgatgcactg gtggcccagg cacacgccca
      181 gaataggcac ggtcgtgtgg aattccctga tgatgttgca ggaaactccg gcgtcgaccg
      241 ggctccccgg gcccggcgag atcacgatgc ccgtgggcgc catctcccga acctcgtcga
      301 cggtaatcgc gtcgttgcgc accaccatga ggttgggatt cagctggccg aagtaatcga
      361 ccaggttgta ggtgaacgag tcgtagttat cgataaataa tatcattgat gcctcccgag
      421 gcctgtttga tggcgttgcg cagggccatg cccttgttca gcgtctcctg gtactcgctc
      481 gccgggacgc tgtcggccac gatgccggcg ccggcctgca ggtggagctt cccgtccttc
      541 gcaagcatgg agcggatggt gatgcacatg tccatgttgt tgcggtagtc gaagtagccg
      601 atggaccctg cgtagaagcc gcgccggtcc ggctcgatgt cgttgatgat ctccatcgcg
      661 cggatcttcg gcgctcccga gaccgtgccg gccgggaacg aggcgcgaaa ggcgtccacc
      721 gccgtcaggt ggggcttgag ctcgccgcgc acgcgcgaga tgatatgcat cacgtgcgag
      781 tatttttcca cggtctcgaa atcgagcacc cgcacgctgc cgtggcgcga tacgcgtccc
      841 atgtcgttgc gcgcgaggtc cacgagcatt atatgctcgg ccagttcctt ggggtcggcg
      901 aggagcgatt cggcatgccg gttgtcctcc tcgatggtgg cgccgcgcgg tcgggtgccg
      961 gcgatcggga tgatctcgag ttcgttgttc tccagccgga tgagcggctc gggcgacgag
     1021 ccgacgagct ggtaatctac gaaatccaga tagaacagat acggcgacgg gttgattccc
     1081 cgcaggctcc gataggcctg gaagacgtcg ccggtgtagt cgatggtgaa gcgctgcgag
     1141 aggaccacct ggaagatgtc cccggcaaag atgtgctcct tcgcccggac cacggcgcgc
     1201 tcgaactcct cctgcgtgaa attggccgcg aaactcgcct cgtcggcgtg gaactcggtc
     1261 ttcggggtca ccggcgcgtt cagcttgccg atgatcgtgt ccagccgatg gcccgcgtcg
     1321 cgatagagcg tcggcacgtg gctgtcgtcg tccacgaaga cattcgcgat caggatgatc
     1381 tcgtttttca ggtggtcgat ggcgatgagg ttgtcataga aggccagcga cgcgtcgtcg
     1441 caatcgaccg gcgtccgccg cgattcgggg atccgctcga tctggcggat catgtcgtag
     1501 ccgatgaagc cgacggcccc gcaggtgaac cgcgagatgc cctccaccgg caccgagcgg
     1561 tagttttgca actcgcgctt gaggacgtcg aaaaagttct cgtcgagcgt gcggttcgcg
     1621 cggaggtcgc cgatcacggt cttcccgccg gtggacttga tcgtccggaa gggcgagtgc
     1681 ccgatgaacg agtagcgccc gatgttctcg cccttctgca cgctttcgag caggaacgac
     1741 gcggtccccg cctcgcggat cttgagatag gccatcaccg gggtgatgaa atcggaattg
     1801 aatttgcgga acacggggac gatggttccc ggcttcgcca gcgcttcgaa ttgttcctgc
     1861 gtcatatggt tcacctgttc ggtagacata aaaaagcccg cttgttttca agcgggcttt
     1921 cgtgaatcag caaatactac tattgctaca ttgacatagc acgcccgctc cgatgttgga
     1981 tgcgccacca ccagttccag gccccgaatg tcgaagtgaa tgcgttcatt cccataaatc
     2041 cttgctatgc gtcacccgta tagcgcgcta tgcgaaatgg caagaaaaat aatatttggt
     2101 attgacaaaa cccgcatagt gcattagatt aaactaagtc tagtttatta aatgtgctat
     2161 gattattgtc aatacacata aggcaaaaac aaacctctcg atgctgctcg cccttataga
     2221 ggaaaagggg gagattgtgc atatctgcag gaatggcaag ccgatagccg aacttcggcc
     2281 cgtggccagt gcgcatgatc cgttgaagat ggatccgcag ctaaaaaaag tcgtttttaa
     2341 cgagagtccc agttcgccgc tcagtgaaaa cgactggccc ggtgataggc gataatgctt
     2401 atgctcgata cctgcaccct gctctggctt gcctccaggc aggaagacct gtccgccgcc
     2461 gcacgcgcgg cgattcggga ttcgtccggc ggactctatg tttcggcgat aagcgcgttc
     2521 gaaatcgcga taaaaagcaa gaaaggcgcc ctggcgcttc cgatgccggc ggatgcgtgg
     2581 ttcacccggg cgctttcgca ccatggcatt gcagagattt cggtcgatgc gcgtcttttc
     2641 ggtgaagcgg ttcgactgcc cgatcatcac aatgatcccg ccgatcgaat catcatcgcc
     2701 accgctctcg ctttctcgat gcgtatcgtt acccccgatc ccctgtttgg ccgctattcc
     2761 ggcgtgataa ctgtttggtg aggatcaaca cgatgaatca gatgcctgaa gtcctcaccc
     2821 agaacatcgg cgatgccgac atccagtatc ttcattaccc cggcgacggt cccgtcgtca
     2881 tcatgctgca cgcgacgggt ttccttccct ggatgtggca tcccatcgcg cgggagctgt
     2941 cgcgcgggta tcgggtcatc gcgccctatt tctgcgacca tcggcatgcc gagccggagg
     3001 agggcgtcga atggatgctc ctggccgacg acctctgccg cctgtgcatg ggactgggca
     3061 tcgagcggcc gtatctagtg ggtcattcca tgggcgccac ggtgatgacg ctggccgagg
     3121 cgatccacgg gccgacggcc cgcgcgctgg tgctcatcga gcccatattc ctgcccgggg
     3181 agatctactc gatggggctc accatcgacc agcatcccct cgcgtcgaag tcgatcaagc
     3241 gacggaacca gtgggacggt cccgacgagg cgcgggacta cctgcgctcg aaggcactgt
     3301 ttaaaaactg ggacgatgag atgctggacc tctatgtgca atacggcatg gtccccggtg
     3361 agaccggcgg cctcaatctc gcctgccacc cgcggcgcga ggcggcgctg ttcatgggtt
     3421 ctcagaagca caatccgtgg ccgctcctcc cggatgtgtc ttgccccgcg cttgtcgtgg
     3481 agggcgggga gagcgagaac cggcatttca tcgacctgaa gaaagcggct tcgctcatac
     3541 cggagggacg atatcatctc gtcgctggag cgggccacct ggtgcccatg gagcggcccg
     3601 gcgaggtcct gtcgtttgtg caggagtttt tcgaatccgt tccgcgatga cacgatgcgg
     3661 caggactcct cgatagcttg ccatcggaaa ataagagcgc cactgcagca gtggcgctct
     3721 cgcgttcgag attagagaat acgactacga ctacgacgcc ttctccttct tccgttgctc
     3781 caactcctcg gcacggagct ccttgcggag gatcttcccg acggtcgact tgggaaggtc
     3841 gtcgcggaac tcgacctcgg tggggagttt gtacggggcg agcttgtttt tgcagaagcc
     3901 gatgatttcg tcttcggtgg cggtttcgcc gggcttgagg accacgaagg ccttgatcga
     3961 ttcgccgcgc gtcgcatgcg gcacgccgat tgcgcacgcc tcgagcacct tcggatgggc
     4021 atcgagacaa tgctcgacct cggtcggcga gacgttctcg gcgctgacga gcagcaagtc
     4081 ccgcgcgcgc gcattgatgt agaggagccc gtcttcgatg cgcgcgacgt cgcccatcgc
     4141 gagccagccg ccgggtttga gcgcggcagc ggtcgcctgc ggatcgttcc agtagccgag
     4201 catgacgtag gggctgcgga cgtggacctc
//
LOCUS       DAORHF010000305         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_22728_length_4203_cov_5.433703, whole genome shotgun sequence.
ACCESSION   DAORHF010000305 DAORHF010000000
VERSION     DAORHF010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22728_length_4203_cov_5.433703"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="PKJ16_17230"
     CDS             complement(<1..941)
                     /locus_tag="PKJ16_17230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HNV48789.1"
                     /translation="MNDAFARELRAGRRVTRDEAAGLFAEDLLELGAIADARRRLVHP
                     EETVGFIVDRIINFTNICEAKCRFCAYHARAGVIEPYDLRLDEIIEKADALARAGGTQ
                     IMLQGGLNPEYTIESYVTMLRAVKERHPDIALHSFSPAETVHLAKRHGMPLADVIGML
                     KHAGLDSMPGASDLLVDRVRRSVSPRKCTVDEWRAVMDALAAHGLFSSATMTYGMGET
                     TDELVEHLDVVRSTQERTGVIRAFIPWSFSPANTALSDVEPATGTDFLRIVAIARIYL
                     DNVRYIQSGWLTEGLPLAQLALAMGANDMGGVLTEEVV"
     gene            complement(1011..2090)
                     /locus_tag="PKJ16_17235"
     CDS             complement(1011..2090)
                     /locus_tag="PKJ16_17235"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV48790.1"
                     /translation="MAENIYHTLRDVLDTIPNGFPATESGVEIKILKKIFTEEEAALT
                     AQLKLKWETPDQIAERTGLDIDYLKKMLPIMDQKGQLMSINLGAMSVYRLMPFVFGIY
                     EFQLKRIDRELAELFEEYAMTAFGKDFFDRSPALMKVVPIESEIDASSSIEPYESVTK
                     LIERAKSFMVNECICKTERALMGHPCSKPTEVCLGFAPIENYFDDGTHGRPISKEEAL
                     DILKKSEEAGLVHMTSNTKKGHFYICNCCSCCCGPLIATNLISTKATAKSNYLAAVDA
                     ESCTACGLCIDRCQVNAITVDDVAVINDRCIGCGLCATTCPAASITMKRRDESDIPPV
                     PRNELDWMAQRAAARGQNDDYKKLL"
     gene            complement(2115..2774)
                     /locus_tag="PKJ16_17240"
     CDS             complement(2115..2774)
                     /locus_tag="PKJ16_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48791.1"
                     /translation="MPPRISFSKEEIIQAAIDVVRADGPNSASARSIAKRMGGSTQPI
                     YRAFETIEDLGRELVAAAEEIAIRHMLEYQYGEWHFLSMGLGFLEFAIREPELYRLLY
                     LSGKNKFQFQHDRPPYDRLFEKMKRDAALAELDDAARVRLFRDMAIYSHGLCGAALMR
                     PGGFTVEEYRPLLMEMGMRLIVFEVLRRRGTLTDAAIRTCLHETAHPIAREILSPSAT
                     S"
     gene            2974..3753
                     /locus_tag="PKJ16_17245"
     CDS             2974..3753
                     /locus_tag="PKJ16_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48792.1"
                     /translation="MIEAIGVREHIRCLEGVKDDIVAFHESAARPDDALRRIWIADVR
                     ELYGVVLAAAELLPSIAGGSDENAGACVQFLGAAESLNRQVLSELKGVRSKASRRLQR
                     RLAEAFESCHDRIAETVRPFVRANDGQPPVATVSRAGDTEFHLHCAVCGEVSFVFRIG
                     VPRFSTEECLVYEGITHAGGVDRKHARRIFTLLDAGAVGRAHSYLMRKKLLEDGIDAY
                     CPECDAVYCREHYHANEEWDDGFYDCTTGTCPRGHHRIIDD"
     gene            complement(3779..>4203)
                     /locus_tag="PKJ16_17250"
     CDS             complement(3779..>4203)
                     /locus_tag="PKJ16_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HNV48793.1"
                     /translation="VARAKDKDNRFRLFGFGHRIYKNYDPRAIILKGMCDTLLKKFNI
                     SEPLIDIAMELESAALADDFFIERKLYPNVDFYSGILYRMIGIPTNMFTVMFAIGRLP
                     GWIAQWKEMHDSVPLKIGRPRQLYTGPPLSDYVPMDQR"
BASE COUNT          717 a         1257 c         1409 g          820 t
ORIGIN      
        1 accacctcct cggtgagcac gccccccatg tcgttcgcgc ccatcgcgag cgcgagctgc
       61 gcgagcggga gcccctcggt gagccagccg gattggatat agcggacatt gtccagatag
      121 atgcgcgcga tcgcgacgat ccgcaggaag tccgtgcccg tcgcgggctc gacgtcggaa
      181 agcgccgtgt tggcgggcga gaaggaccag gggatgaagg cgcggatgac gccggtgcgc
      241 tcctgcgtgc tccgcacgac gtcgaggtgc tcgacgagct cgtcggtcgt ctcgcccatg
      301 ccgtaggtca tcgtcgcgct ggagaaaaga ccgtgcgccg ccagcgcgtc catcaccgcg
      361 cgccactcgt ccacggtgca cttgcgcggg cttacgctgc gacgcacgcg gtccacgagg
      421 aggtcggacg cgccgggcat ggagtcgagc ccggcgtgct tcagcatgcc gatgacgtcg
      481 gcgagcggca tgccgtgccg cttcgcaagg tgaacggtct cggcgggaga aaatgagtgg
      541 agcgcgatgt ccggatgtcg ttccttcacg gcgcgcagca tcgtcacgta cgattcgatc
      601 gtgtactcgg gattgagccc gccctgcagc atgatctgcg tgccgcccgc gcgcgcgagg
      661 gcgtcggcct tttcaatgat ttcgtccagg cgcaggtcgt acggctcgat caccccggcc
      721 cgggcgtggt aggcgcagaa gcggcacttc gcctcgcaga tattggtgaa gttgatgatg
      781 cggtccacga tgaagccgac ggtctcctcc ggatgcacga gccgccggcg ggcgtcggcg
      841 atcgcgccga gctcgaggag gtcctcggcg aaaaggccgg cggcctcgtc gcgcgtaacg
      901 cggcggccgg cgcgaagctc gcgggcgaag gcgtcattca tacggcggta tcggcctccc
      961 tgaaggtgtg gcaatgatac aagccgcact ctgcgcgcgg ggccgaatcg tcacaacaac
     1021 tttttgtagt cgtcgttctg gccgcgggcc gcggcgcgct gggccatcca gtcgagctcg
     1081 ttccggggaa cggggggtat gtcgctctcg tcgcgtcgct tcatggtaat ggacgctgcg
     1141 gggcaggtgg tggcgcacag gccgcagccg atgcagcgat cgtttatcac ggcgacatca
     1201 tcgacggtga tggcgttcac ctggcagcga tcgatgcaca atccgcaggc ggtgcagctc
     1261 tcggcatcga ccgccgcgag gtagttcgat ttcgcggtcg ccttggtgct gatgaggttt
     1321 gtcgcgatga ggggcccgca gcagcacgaa cagcaattgc agatgtagaa atggcccttc
     1381 ttcgtgttgg aggtcatgtg gacgagcccc gcctcctcgg actttttgag aatgtccagc
     1441 gcctcctcct tgctgatggg ccgcccgtgc gtcccgtcgt cgaagtagtt ttcgatgggc
     1501 gcgaagccca gacacacctc ggtgggcttg ctgcatgggt gtcccatgag ggcgcgctcc
     1561 gttttgcaga tacactcgtt caccatgaac gacttggcgc gctcaatgag cttggtcacg
     1621 ctctcgtagg gctcgatgct ggatgacgcg tcgatctcgc tttcgatggg caccaccttc
     1681 atgagcgcgg gcgagcggtc gaaaaagtcc tttccgaagg cggtcattgc gtattcctcg
     1741 aaaagctcgg cgagctcgcg gtcgatgcgc ttgagctgga actcgtagat gccgaagacg
     1801 aagggcatga ggcggtacac cgacatcgcg cccaggttga tggacatcag ctgccccttc
     1861 tggtccatga tggggagcat cttcttcagg tagtcgatgt caagccccgt gcgctcggcg
     1921 atctggtccg gcgtctccca cttcagcttg agctgcgcgg tcagcgccgc ctcctcctcg
     1981 gtgaagattt ttttcaggat cttgatctcc acaccgctct cggtggcggg gaaaccgttg
     2041 gggatggtgt cgagcacgtc gcgaagggtg tgatagatgt tctcggccat ggcaatctcc
     2101 tttgtgggcg ttcgttatga tgtcgcggaa ggcgatagta tctcgcgcgc gatcgggtgt
     2161 gcggtctcgt gcaggcaggt gcgaatcgcc gcgtcggtga gcgtcccccg gcgtcgcagc
     2221 acctcgaaga cgatcagccg catccccatc tccatgagca gcggccggta ttcctcgacg
     2281 gtaaagcccc cggggcgcat gagcgccgcc ccgcagaggc cgtgggaata gatcgccatg
     2341 tcgcggaaga gccggacgcg ggccgcgtcg tccagctcgg cgagggcggc gtcgcgcttc
     2401 atcttctcga agagccggtc gtaggggggg cggtcgtgct ggaattggaa cttgttcttt
     2461 ccggaaagat agagcagccg atacagctcg ggctcgcgga tcgcgaattc caaaaagccc
     2521 agccccatgg acaggaagtg ccactccccg tactggtact ccagcatgtg ccggatggcg
     2581 atctcctccg ccgcggcgac gagctcccgg ccgaggtcct cgatggtctc gaaggcgcgg
     2641 tagatgggct gggtggatcc gcccatgcgc ttcgcgatcg agcgcgcgct cgcgctgttg
     2701 ggcccatcgg cccgaaccac gtcaatcgcg gcctggatta tctcttcctt cgagaagctt
     2761 attcgtggcg gcatggcata tcatacataa ataacatgtg taattataaa taactaatgt
     2821 tatttaaaaa gtcaagcatt tttttagggt gatatgattt tgcttcgcaa tgcgccatgt
     2881 gacatgaact ttctgtttga catgccatgg gcggggggat atcttgaggc tatcttcctc
     2941 gaaaagcgaa aaatcttaaa taaccggaca ggtatgatag aagcgatcgg cgttcgcgag
     3001 catatcaggt gcctggaggg cgtgaaggac gatatcgtcg cctttcacga gtcggccgcg
     3061 cgtccggacg acgcactgcg ccgtatctgg atcgccgatg tcagggagct ctatggcgtt
     3121 gtgctggccg cggcggagct gttgccgtcg atcgccggcg gcagcgacga gaacgccggc
     3181 gcctgcgtcc agttcctcgg cgcggccgag agcctcaacc ggcaggtgtt gagcgaactg
     3241 aagggcgttc gcagcaaggc gtcgcgtcgg ctccagcggc ggcttgccga ggcgttcgag
     3301 agttgccatg accggatcgc cgaaaccgtg cgtcccttcg tgcgcgcgaa cgacggccag
     3361 ccccccgtgg cgacggtctc gcgcgcgggc gataccgagt ttcatctgca ctgcgcggtc
     3421 tgcggcgagg tgtcgttcgt gttccgcatc ggcgtgccgc gcttctccac ggaggagtgc
     3481 ctcgtctacg aggggatcac ccatgccggc ggtgtcgacc ggaaacacgc ccgccggatc
     3541 ttcaccttgc tcgatgcggg cgccgtcggc cgggcgcatt cgtatctcat gcgaaagaag
     3601 ctcctcgagg acgggatcga cgcctactgc cccgaatgcg acgccgtgta ctgccgcgag
     3661 cactatcacg cgaacgagga gtgggacgac gggttctacg actgcaccac cggcacctgc
     3721 ccgcgcggac atcatcgcat catcgacgat tgacgggggc gcggcggcgc agacgcgatc
     3781 agcgctggtc catgggcacg tagtcgctca gcggcggtcc cgtgtagagc tggcgcggcc
     3841 ggccgatctt cagcggcacg ctgtcgtgca tctccttcca ttgcgcgatc cagccgggca
     3901 ggcgcccgat ggcgaacatc accgtgaaca tgttggtggg gatgccgatc atccggtaga
     3961 ggatgccgct gtagaagtcg acgttcggat acagcttgcg ctcgatgaaa aagtcgtcgg
     4021 cgagcgcggc ggattcgagc tccatggcga tgtcgatgag cggctcgctg atgttgaatt
     4081 ttttcagcag cgtgtcgcac atgcccttga ggatgatcgc gcgcgggtcg tagttcttgt
     4141 agatgcgatg gccgaacccg aagagccgga agcggttgtc cttgtccttc gcgcgcgcca
     4201 cgc
//
LOCUS       DAORHF010000306         4155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_23142_length_4155_cov_3.721707, whole genome shotgun sequence.
ACCESSION   DAORHF010000306 DAORHF010000000
VERSION     DAORHF010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23142_length_4155_cov_3.721707"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="PKJ16_17255"
     CDS             complement(<1..1816)
                     /locus_tag="PKJ16_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HNV48794.1"
                     /translation="MKQLLVHIVAIIITLTATGLVAQDWPIFKGNIYFTGNNDEVIVK
                     NNNLKWLYQADERVFNPVVSDGRVFFSDIKSNVYCLDEEYGKVQWRVDFKQISSQFKA
                     LSRSAGKIKYPLVKDNMLFLSDPIAMYAFDKHSGRVLWARTGMRTETVKPQGLSGRTS
                     AVSVDGIYADPIIMNDKIFYGTRNMFLARDVVNGHELWENRAIKTYSAFPTYYDNMII
                     TQSMDYATGLFTVHCLAYDTGKTIWERRLEKPLQIFPPVVYRQRVYIPTGRSLYCLDL
                     KTGDTRWSKEYGAPITSTPSFTDRAILFTVNNSDIAVINPDSGERIRTIPVGTSAGPL
                     FVTIRDLIYIAYNESVPVKGKPITFGRVRAMNFDDNTVLWEYRTPFPGAVSQPIASKG
                     ILILPAGNYVYALGTEYYARVVDGGDGYAVKPGGPVKPDGPSDREGATKRPAKHEKKS
                     EKTPTGPDRPDDRMTPGREKATQRNEPPKLETRKFKVTITDDNKRGISAQVEIKKWDK
                     DKLVYSKKADISSSGEVEVPTGDNVEILATASGYLPKKTIIGGKDAGADIHLERLEKG
                     KGYVVDNILFDFDKWYLKKASIDIVGKLVMLLKRDPSLR"
     gene            complement(1838..2083)
                     /locus_tag="PKJ16_17260"
     CDS             complement(1838..2083)
                     /locus_tag="PKJ16_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48795.1"
                     /translation="MILHIYYRPQKKKEALFDEFLSRFRERYTRSINEIKFVPYSGAG
                     RLIAVVFAADDVDEHEIGSFLTTEFSGGTLEEFVFQR"
     gene            2393..2665
                     /locus_tag="PKJ16_17265"
     CDS             2393..2665
                     /locus_tag="PKJ16_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48796.1"
                     /translation="MISKRRWYDRDPDLRKVMITLEHADEETRNRIVHEINRLLCEYG
                     CDGIDCKLTDLPLAMNKRWYDCDPYWWITVNCLRYVNADIIKKIAL"
     gene            2760..3629
                     /locus_tag="PKJ16_17270"
     CDS             2760..3629
                     /locus_tag="PKJ16_17270"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HNV48797.1"
                     /translation="MYVHGESPLGAAEKKSLISGAPFDIVCITGFAVMADGNVRVAVN
                     REKLAHISRSSRARGARMYPLIAPRSVSQGKRLLRSVEARDTAIRNLGRILKVFNFTG
                     LHLDFEYFPPDYRHDLAVFIRELKASFPGTTISMAVFPQTGFPSEYARFHDLALLAPL
                     LDEIVLMCYDIHRPGTAPGPVTSVDWARRSIAHARAHVDSRRLYLGIPAYGYRWTKDG
                     RAAVVTGFQGKRLIHSALSVRDPSGTVRLNGRKDGSFVFFADDHTRAVLTALAKEHRL
                     AGIAVWRLGFDDL"
     gene            3783..3998
                     /locus_tag="PKJ16_17275"
     CDS             3783..3998
                     /locus_tag="PKJ16_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48798.1"
                     /translation="MKRKKNNFKKLLQKYLKFKGLDIVIYTIDGTVIELNKNRTMIKD
                     EIVVFDKYNREQRIPISLIKAVDLFAA"
BASE COUNT          859 a         1114 c         1143 g         1039 t
ORIGIN      
        1 gccggagcga cggatcacgc tttaacaaca tgacgagctt gccaacgatg tcgatcgacg
       61 ccttcttgag gtaccattta tcgaaatcga agaggatgtt gtccaccacg tatcctttgc
      121 ccttttcgag ccgctccaga tgaatgtccg ctccggcgtc cttgccgccg atgatggttt
      181 tcttcggcag atacccggag gcggttgcca aaatctcgac attgtcgccg gtgggaacct
      241 caacctcgcc cgatgaggag atgtcggcct tcttcgagta gacgagcttg tccttgtccc
      301 atttttttat ctcaacctgc gcggagatgc ccctcttgtt atcatcggtg atggttacct
      361 tgaattttct ggtctccagt ttcggtggtt cattccgttg tgtggctttc tcccgtcccg
      421 gcgtcatgcg gtcatccggc cggtccggtc cggttggggt tttctcggat ttcttctcgt
      481 gtttcgcggg gcgtttcgtc gcgccttcgc ggtctgatgg cccgtccggt ttcaccgggc
      541 ctcccggttt aaccgcatag ccgtcgccgc catcgacaac gcgcgcgtag tattccgttc
      601 cgagcgcata gacatagttc cccgctggca ggatcaggat gcccttcgag gcgatcggct
      661 gcgaaacggc gccggggaac ggcgttcggt actcccacaa taccgtgttg tcgtcaaagt
      721 tcattgcgcg aacccgtccg aaggtaatcg gttttccctt aacggggacc gactcgttat
      781 aggcgatgta gatgaggtcg cggatcgtca cgaacagcgg tcccgcggat gtcccgacgg
      841 ggatggtgcg gatgcgctcg ccggaatccg gattgatcac cgcgatatcg ctgttgttca
      901 cggtaaacag gatggcgcga tcggtaaaac tgggagtgga ggtgatgggg gcgccgtact
      961 ctttagacca gcgggtgtcg ccggtcttca ggtcgaggca gtacagggag cggccggtgg
     1021 ggatgtacac acgctggcga tacaccaccg gggggaatat ctgcaggggt ttttccaggc
     1081 ggcgctccca gatggtttta cccgtgtcat aggcgaggca atggaccgta aacaggcccg
     1141 tcgcataatc catcgattgc gtgatgatca tattatcgta gtacgtgggg aaggcgctgt
     1201 atgttttgat ggcgcggttt tcccagagct catggccgtt cacgacatcg cgcgccagga
     1261 acatgttccg cgttccgtaa aagatcttgt cgttcatgat gatggggtct gcgtagatcc
     1321 cgtcgacgga aacggcgctg gttcgtcccg agaggccctg cggtttcacg gtctcggtgc
     1381 gcatgccggt tcgcgcccag agcacgcggc cgctgtgctt gtcgaaggca tacatggcga
     1441 ttggatcgga aaggaagagc atgttgtctt tgaccagggg atatttgatc tttcccgcgg
     1501 acctcgacaa tgccttgaat tgcgacgata tctgcttgaa gtcgacccgc cattgcacct
     1561 ttccgtattc ctcatcgagg cagtagacat tcgatttgat gtcgctgaaa aagacccggc
     1621 cgtccgacac caccggattg aacacccgtt cgtcggcctg gtagagccat ttcaggttgt
     1681 tgttcttgac gatcacctcg tcattgttgc cggtgaaata aatattgccc ttgaagatgg
     1741 gccagtcctg cgcaaccagg ccggtggccg tgagcgttat gataatcgcg actatgtgga
     1801 cgaggagctg tttcatcacc gtgtaccgtt ccgtgggtta tcgctgaaag acgaattctt
     1861 cgagggtccc gcccgaaaac tctgtcgtga gaaatgagcc tatttcgtgc tcgtcgacat
     1921 cgtccgccgc aaagacgacc gctataagcc ggccggcgcc ggagtacggc acgaacttta
     1981 tctcgttgat agacctggtg taccgttccc gaaaacggga gagaaactca tcgaagagcg
     2041 cctctttctt tttttgcggt cgatagtata tgtgcaggat catgatacgg taaccatgtg
     2101 cgcccggatt cgatttttca agtaaatatt atgcatgcgg aggcccaatg tgccattcga
     2161 gattgttata tgcaacttcc cataacatat cttatgtctc ataaggcggg atgtgctttt
     2221 agggattgtc gcaaatttcg ccgttcttat cggatttact tttcttttct cttggatttc
     2281 aatatatttc gtatgatatt tcccgggatg tttcaggaac ttgtgcgata tcaaatccca
     2341 ggcgatacta ctgtatatac aaaaaaccgc acacccccag gaggcgttgg gtatgatcag
     2401 caagcgtcga tggtatgacc gggacccgga ccttcgcaag gttatgatta ccctggagca
     2461 tgctgacgag gaaacacgga acaggatcgt gcacgaaatt aaccggcttc tgtgcgaata
     2521 cggctgtgac ggcatcgact gcaagcttac ggatcttccc ctcgccatga acaagcgttg
     2581 gtacgattgc gacccctatt ggtggatcac ggtcaattgc cttcggtacg tgaatgccga
     2641 tatcatcaaa aaaattgcac tgtagacgat ttctcgcgat attccccgtt attacacttt
     2701 tcatcacagc agcgtacggc cgtgctgacg cccgagatac agctgcattt cggagcatga
     2761 tgtacgtcca cggagaatcg ccgctcgggg ccgctgaaaa aaaatcgctg atatcgggcg
     2821 cgccgttcga tatcgtgtgc attaccggat ttgccgtcat ggcggatggg aacgttcgcg
     2881 tggccgtaaa ccgtgagaag cttgcgcata tcagtcgatc gagtcgggcc cggggggcgc
     2941 gcatgtatcc cctgatcgcg cctcgttcgg tttcgcaggg gaagcgtctt ttacgctcgg
     3001 ttgaagcgcg cgataccgcc attcggaatc tggggcgcat tctgaaggtt ttcaacttca
     3061 cgggattgca cctcgatttc gagtactttc cgccggatta tcgacacgat cttgccgttt
     3121 ttatccgcga gctgaaagcg tcgtttcccg gaaccacgat ctcgatggcg gtattcccgc
     3181 agacgggatt cccgtccgaa tacgcccgat ttcacgatct cgccctactc gcgccccttc
     3241 tcgacgagat cgtgctcatg tgttacgata ttcatcgtcc gggaacggcg cccggcccgg
     3301 tcacctcggt cgactgggcg cgtcggagca tcgcacatgc gcgcgcccac gtggattcgc
     3361 ggcgcctgta tctcggcata cctgcatacg ggtatcggtg gacaaaagac ggacgggctg
     3421 cggtcgtcac cggatttcag ggaaagaggc tgattcactc cgcgttatcc gtgcgcgatc
     3481 cctccggcac ggtgcgattg aatggtcgga aagacggatc gttcgtcttt tttgcggacg
     3541 atcatactcg cgccgtcctc acggcccttg ccaaggagca tcgtctcgcc ggaatcgccg
     3601 tctggcggct cggatttgat gatttgtaac caaaactttt attaaacata aaaatattcc
     3661 cgtttattat aattttttta acgtattttg gcgaatcacc cgatactttt tatgagacat
     3721 aatggattgg tggcgtgttg aggatacgga ggtcccgacc attgctatgc tggaggttct
     3781 ccatgaaacg caaaaagaac aacttcaaga agctgcttca aaaatacctc aagttcaagg
     3841 ggctggacat cgtcatctac accatcgacg ggaccgtgat cgaactcaac aagaaccgga
     3901 ccatgatcaa agacgagatc gtcgtcttcg acaagtacaa ccgggaacag cgaataccga
     3961 tttcgttgat caaggcggtc gatctcttcg ccgcatagcc gctgacggaa tcctccgacg
     4021 cccttctctc tcgcgccgtc cgcatgcgcc cgacccgttc atccgtcatg gatgttcgga
     4081 tcggagctac catgcgcatt ttgaccaatg tgcggcaata ttttccgtaa atagtattga
     4141 caataatcac acctc
//
LOCUS       DAORHF010000307         4058 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_23928_length_4058_cov_5.011242, whole genome shotgun sequence.
ACCESSION   DAORHF010000307 DAORHF010000000
VERSION     DAORHF010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4058
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23928_length_4058_cov_5.011242"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1720)
                     /locus_tag="PKJ16_17280"
     CDS             complement(<1..1720)
                     /locus_tag="PKJ16_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HNV48799.1"
                     /translation="MADYQDTSMAAVFQRNAAKYADKSYASYKTDSGWKDISWGSMNT
                     MVHNLAYYLLSIGIGKDDKVGLFSPNRWEWHLAALAINSIGAVDVPIYATNSAEESEY
                     ILGNSDAKACFVGTADHLKKILSIRTKIPNVKQIVVFDEVDVNQTEELTLSKALSLGE
                     ASPNKDLFDQRLAAIQPNDLSTLIYTSGTTGNPKGVMLTHNNFVSNVRNTLAEMKDPK
                     TGEDLLGPNDHYLSFLPLSHSLERTAGFHGAIWMGARTAFAEDITKLLDYFQEVRPTI
                     IISVPRIYEKVHAGILAKVAGAPGLKKKIFNFAMRQAAKNLPHVCRDIPRSGLFAFKY
                     NLADKLVLSKLKVALGMDKLRYAISGGAPLSQSDAEFFIGMGFKILEGFGLTETTPVT
                     NFNRPWFIKPGTVGQPIPETEVKIADDGELLIKGPQVMAGYYKNDEATKEAIRSDGFF
                     RTGDIGIIDEDGCLRITGRIKDIIVTAGGKNISPQNIESSIKESAFVEQIGVIGDKRK
                     YLSALIVPAFPELERWAGENGVTFGSRADLIANEKVQALIRAEIDKYTAQYARVEQIR
                     KFTLLDT"
     gene            complement(2186..3013)
                     /locus_tag="PKJ16_17285"
     CDS             complement(2186..3013)
                     /locus_tag="PKJ16_17285"
                     /inference="COORDINATES: protein motif:HMM:NF025055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HNV48800.1"
                     /translation="MDTNTRLHAAAIRRYVTYGIIAALILGMAVFQFITARQQEFDGV
                     RINLAGRQRMLSQKILSGILLYDDGRETRSAVEKNIAVFDSALRALIDGGTAPLDLAG
                     DTVRTLRPIDNTSIRTQMLRVMETWRPFRDRCEEFIERRDRAALQYLIANNFALLDQI
                     DRTAYLLQVESERKNSIITGVLAAEIALIFLLFGLILSRYILRLRNVEAHIRDLEKLL
                     PICSACKKIRIDDAHPENMRSWISIEKYLHESKINLTHGICPECAARLYPDLSPYKK"
     gene            3293..>4058
                     /locus_tag="PKJ16_17290"
     CDS             3293..>4058
                     /locus_tag="PKJ16_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HNV48801.1"
                     /translation="MAARKKQVISEAGLVKGFIEYLRGLFYIDPDTDQKGTVESITRG
                     VEFRGIGLWTLIFAIYIASIGLNTNSTAVIIGAMLISPLMGPIMGAGLALGIYDFELL
                     KKSLRNLAVMTGISIATSAVYFYLSPLSDAQSELLARTTPTIYDVLIAVFGGAAGIVA
                     GSRKEKISNVIPGVAIATALMPPLCTAGFGLATGNLRFFAGAFYLYLINSVFIGMSTL
                     IFVRYFRFKRKTYLDRAVERKIHRYVLVFSVLFILPS"
BASE COUNT          738 a         1115 c         1278 g          927 t
ORIGIN      
        1 cagtatccag cagggtgaac ttgcggatct gttccacccg ggcgtactgc gcggtgtact
       61 tgtcgatctc agcgcggatg agcgcctgca ccttctcatt ggcgatgagg tcggcccgag
      121 atccgaacgt gacgccgttt tcgcccgccc agcgctccag ctccgggaat gccgggacga
      181 tgagcgccga caggtacttg cgcttgtcgc cgatgacgcc gatctgctcc acgaacgccg
      241 attccttgat gctgctttcg atgttctgcg gggagatgtt cttcccgccg gcggtcacga
      301 tgatgtcctt gatgcgtccg gtgatccgta ggcaaccgtc ctcgtcgatg atgccgatgt
      361 cgcccgtccg gaagaacccg tcgctccgga tggcctcctt ggtggcctca tcgttcttgt
      421 agtagccggc catcacctgc ggccccttga tgaggagctc gccgtcgtcg gcgatcttca
      481 cctcggtctc ggggatcggc tggcccacgg tgcccggctt gatgaaccac ggacggttga
      541 agttggtcac cggcgtggtt tcggtgaggc cgaagccctc gaggatcttg aagcccatgc
      601 cgatgaagaa ctcggcgtcg gattgcgaaa ggggcgcacc gcccgagatc gcataccgca
      661 gcttgtccat gcccaacgcg accttgagct tggacaggac gagcttgtcg gccaggttgt
      721 acttgaaggc gaacaggccg gagcggggaa tgtcgcggca cacatgcgga aggtttttcg
      781 ccgcctgccg catggcgaag ttgaatatct tcttcttgag ccccggcgcg ccggcgacct
      841 tcgccaggat gcccgcgtgc actttttcgt agatacgggg aacgctgatg atgatggtgg
      901 ggcgcacctc ctggaaataa tcgagcagct tggtgatgtc ctccgcgaag gccgtgcgcg
      961 cgcccatcca gatggcgccg tggaagcccg ccgtccgctc gagcgagtgc gagagcggga
     1021 ggaaggacag gtaatggtcg ttggggccga gaaggtcttc gcccgtcttc ggatccttca
     1081 tctcggcgag cgtgttgcgc acgttcgaga cgaagttgtt gtgtgtgagc atgaccccct
     1141 tgggattgcc cgtggtcccg gaggtgtaaa tgagcgtcga gaggtcgttc ggctggatcg
     1201 ccgcgaggcg ctgatcgaag agatccttgt tggggctcgc ctcgcccagc gacaatgcct
     1261 tcgacagcgt cagctcctcg gtctggttta cgtcaacctc gtcgaatacg acgatttgct
     1321 tcacgttcgg tatctttgtc cgaatggaga gtatcttctt cagatggtcc gcggtcccca
     1381 cgaagcaggc cttggcgtcg gagttgccca ggatatactc ggactcctcc gccgagttgg
     1441 tcgcgtagat gggaacgtcc accgcgccga tggagttgat cgcgagggcg gcgaggtgcc
     1501 attcccagcg gtttggcgag aacagtccca ccttgtcgtc cttgccgatg ccgatggaga
     1561 gcaggtaata cgcgaggttg tgcaccatcg tgttcatcga gccccagctg atgtccttcc
     1621 agccgctgtc ggtcttgtag gatgcgtacg atttgtcggc gtatttcgcg gcattccgct
     1681 gaaaaaccgc cgccattgag gtgtcttggt aatcagccat catggcctcc tttgtgaaaa
     1741 gatgtacggc gtatatagtt aatgcccgtc cgcataatag aagcgcggaa gcgcatgttg
     1801 ctgagccatg cgatgaatac tatcggtaat gaggagaacg ggatgacggt ttacccgtcg
     1861 aatgcgtctc tatagcgcgg aatgaccact gttacccgat cacatttcgt tcagaatgag
     1921 taaaatttgt caaggcgaaa tgcagcggaa ctgcagaaaa atgcgtgcgt tcgccatgtc
     1981 catgcgtacc tttgttaatg cgacaaagct cgcttcgatg tgcatgaaat gcgcacgtct
     2041 gatcatgcat ttccaatacg gccgattgtc ggacgcattg ttgattacat cgtcattgcg
     2101 tgtgaagcaa gcggcattac gtacgaacct attccagcag ggagccgaat gagacggcag
     2161 gcgtgaatcc ccttgtgggg cgacgctact ttttgtatgg agatagatct ggatagaggc
     2221 gcgccgcgca ctcggggcat atgccgtggg tgaggttgat cttgctctcg tgcaggtatt
     2281 tctctatcga gatccacgag cgcatgttct cgggatgcgc gtcgtcgatg cggatcttct
     2341 tgcaggcgct gcatatcggg agcagcttct ccaggtcccg gatgtgcgcc tcgacgttcc
     2401 ggaggcgcag gatgtagcgc gacaggatca gcccgaacag caggaagatg agcgcgatct
     2461 ccgcggcgag gacgccggtg atgatggagt ttttccgttc ggattccacc tgcagcaggt
     2521 atgcggtccg gtcgatctgg tcgagcaggg cgaaattgtt ggcgatcagg tactgcagcg
     2581 cggcgcggtc gcggcgctcg ataaattcct cacaccggtc gcggaagggg cgccatgtct
     2641 ccatcacgcg taacatctgt gttctgatgg atgtgttgtc gatggggcgc agggtgcgga
     2701 ccgtgtctcc cgccaggtcg agcggggccg tgccgccatc gattagggcg cgcagcgcgc
     2761 tgtcgaagac ggcgatattt ttttccaccg cggagcgcgt ttcccggcca tcgtcgtaca
     2821 ggaggatgcc gctcaggatc ttttgcgaca gcatgcgctg ccgccccgcg aggttgatcc
     2881 gcacgccgtc gaattcctgc tggcgtgcgg tgatgaactg aaacaccgcc atcccgagga
     2941 tgagcgccgc gataatcccg tatgtgacgt accggcgaat tgcggcggcg tgcagtcgtg
     3001 tgttcgtgtc caagtccgct ccgtatgcgc tggagtgtga accgctctgc gcgatcggtc
     3061 catgaacact atgtataccg acgagtgcgt gcggtgacaa gtttttttgt ttttttgcat
     3121 tgttgttcgc gtgattttta tggagcgctg cgctgcgatc gattatcgga ccgccggtcg
     3181 acggccgcct gacggtgaag cgcggtatgc ccaggacgct ggaatgtgct tgacgttcga
     3241 tcgcgaacca gatcaaactt ataccgaatg atgtggacac tgggaggtgg gcatggccgc
     3301 gaggaaaaag caggtgatct ccgaggcggg actcgtaaag ggctttatcg agtatcttcg
     3361 cggcctgttt tatatcgatc cggacaccga ccagaagggg accgtcgagt ccatcacgcg
     3421 gggcgtggag tttcgcggga taggattatg gacgttgatc ttcgcgatct acatcgcctc
     3481 gatcgggctc aataccaact cgacggcggt gatcatcggc gccatgctca tatcgccgct
     3541 catggggccc atcatgggtg cgggcctcgc gctgggtatc tacgatttcg aactcctgaa
     3601 gaaatcgctg cgcaatcttg ccgtcatgac gggcatcagc atcgcgacct cggccgtgta
     3661 tttctatctt tccccgttgt cggatgcgca atccgagctt ttggcgcgca ccacgccgac
     3721 catctacgac gtgctgatag ccgtatttgg cggggcggcg gggatcgtcg ctggatcgcg
     3781 caaggagaag atttcgaacg tgatccccgg cgtggctatc gcgacggcgc tcatgccgcc
     3841 gctgtgcacg gcggggtttg gcctcgccac cggaaacctc aggtttttcg ccggcgcgtt
     3901 ttatctttat ctcatcaaca gcgtgttcat cggcatgtcc acgttaatat tcgtccgata
     3961 cttcaggttc aagcgaaaga cctatctcga ccgggcggtg gagcggaaga ttcaccgata
     4021 tgtgctggtg tttagcgtgt tgttcatctt gccgagcg
//
LOCUS       DAORHF010000308         4057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_23940_length_4057_cov_5.215392, whole genome shotgun sequence.
ACCESSION   DAORHF010000308 DAORHF010000000
VERSION     DAORHF010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23940_length_4057_cov_5.215392"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..2750
                     /locus_tag="PKJ16_17295"
     CDS             288..2750
                     /locus_tag="PKJ16_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48802.1"
                     /translation="MENFMNPSAVLRILCLSGCALLTLLLFNSYPVDAADGKKWDGKR
                     YPFRLLNPSQPNGESNPFIIDTAGKLAYFAWLAKSDLDMADYGKKNGLNGLNQAFSKN
                     FVKLAADLDMNGSKFEFVPIPATYVSFDGGGHVIYNLRISDKTTKAITDDVTGEVEMH
                     LALFQGVGMIKNLGIGKGSTITNYGLRKEYPKRAYKVYAAGITVSAWGIDNCFSDATI
                     AVKEDGEAWIGGIAANCESIANSYNRGAITFEGTVINSKEKNKTGKILPGPLWVAGVC
                     AIPAKKMSGCYNTGAITVRASGRDLKIGGVAAETNDTDCMDLNNTGALRVIATGAIKK
                     AHIGGVLGYGSTSRRIYVKPIKYVDRGIFYNKGAIAVTVHTGDSINAGGIIGGDKGGF
                     GASSYAFGGVYGCINAYNTGSISVTSTGTVGALSAGGIAGNLCMVINSYNSGRISGAS
                     GAGTTLYLGGLGGSDVYVQNGYNIGAVSAKGSGTNFTGGVIGHAGEAIWGEVDTALHA
                     SLNGFWLKQSKSGGINSDIKYAKGSYFYKKKSEIKKANSGIGTLIDSFDKEDPNTMIE
                     ASYGAVYSFDGPSASVMTRSDDAKGKRSNLNGTLLDNLNRMVEDRINRLYRRWVVDGT
                     NGGYPVLSPKPTVFSHTSEKPDASVAQRIAGEYWGVHKQWNDRVFLGADGSFRRAKGG
                     DGGAWSFTGKRIILRWAKWEPEILEQKSAGAFSSNVYNFTLKRTGVASPDASVPTDKP
                     VSPLAKKLAGVYYAQHKDWTSSLTINANGSFTRDSNGDRGTWTYDGKTLVLTWDKGAS
                     DTLKKTDTGFYCPAYKFTLRQR"
     gene            complement(2915..3643)
                     /locus_tag="PKJ16_17300"
     CDS             complement(2915..3643)
                     /locus_tag="PKJ16_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HNV48803.1"
                     /translation="MAALSLSIIIAAACGSSGPTTDASAFKYEYFFTYVKKSDYENVR
                     KALRNGFRIDQCDYAGKQAIHWAVINNDGRMMLLLLDHGADINARNRDGDTPLFLFLR
                     FGTERHDDEMIRSLLRNGADVNAKNNAGQTPLHQAAMWGTFERAAVLVRNGAHVNARD
                     HSGNTPLHMAARWGGRSRIAALLLENGAEVNPRGELFTTPLRQAILSGDEKTIALLKE
                     RGAEDFGVIPVRMHKALRNLFFLR"
     gene            complement(3747..3902)
                     /locus_tag="PKJ16_17305"
     CDS             complement(3747..3902)
                     /locus_tag="PKJ16_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48804.1"
                     /translation="MKGNTIVIDPHVKLRDDTEVEITPIELKDPIHGIWTDDRSAEDT
                     IREIGSS"
BASE COUNT          945 a         1026 c         1171 g          915 t
ORIGIN      
        1 gagttgttag aatgatcttt tcgggagctg tgtgctggca gttgtcgttg aacagtataa
       61 ccaatcgctt tccgatctca ttacgcccgt ctgctgctgt ttgtcccgac gcggacagct
      121 cgacatgtag gccgcaacga gcggttgcca catcggcgga gttgaattcg tagaaccgta
      181 ctgatcattt ttgactggcg aggactgacc acgcctgccg gataggctga gtgagcaact
      241 cggacgatat ggagtaatgc atcgaccccg cgcttgatta ttgttgattg gagaacttca
      301 tgaatccttc tgccgtgtta cgcattcttt gtttgtccgg atgcgctctt ctgacgctcc
      361 ttcttttcaa tagctaccca gttgacgccg ccgacggaaa gaaatgggac gggaagcgat
      421 atccgtttag actgcttaat ccgtcgcaac cgaacggcga gagcaatccg tttatcatcg
      481 ataccgcggg taagctggca tatttcgcct ggctcgcgaa atcagacctg gacatggcgg
      541 attacggcaa aaaaaacggc ctcaacgggc ttaaccaggc attttcgaag aatttcgtga
      601 aactcgccgc ggacctcgac atgaatggtt cgaagtttga gtttgtcccc ataccggcca
      661 cctatgtcag ttttgacggc gggggtcatg tgatctacaa tctgcgcatc agcgataaaa
      721 cgaccaaggc gatcaccgat gatgtaaccg gcgaggttga gatgcatctc gccctttttc
      781 agggcgtcgg catgataaag aatctcggta tcggcaaagg gtcaaccatt accaattacg
      841 gcctgcggaa agagtatccg aaaagggcct acaaggtgta tgcggcggga ataaccgtaa
      901 gcgcgtgggg gatcgataac tgtttcagcg atgcgacgat agccgtaaaa gaagacggcg
      961 aggcatggat cggggggatc gcggccaatt gcgaatccat cgccaattcc tataaccgcg
     1021 gcgcaatcac cttcgagggg accgtcatca attcgaagga aaaaaacaaa acagggaaaa
     1081 ttcttccggg accgctctgg gttgccgggg tgtgcgcgat acccgcaaaa aaaatgtcag
     1141 ggtgttacaa cacgggggcc attaccgtca gggcgtcggg aagggatcta aaaattggcg
     1201 gcgttgccgc ggagacaaac gataccgatt gcatggatct taataataca ggcgccctgc
     1261 gcgtaatcgc caccggcgct atcaaaaaag cgcatatcgg cggcgtgctg gggtatggat
     1321 cgacatcccg gcggatatat gtaaagccca tcaaatacgt cgatcgcggt attttttata
     1381 acaagggcgc cattgccgtt acggtgcaca cgggcgactc catcaatgcc ggcggcataa
     1441 tcggcgggga caagggggga ttcggcgcga gctcctacgc tttcggcgga gtctacggat
     1501 gcattaatgc gtacaacacc ggttcgattt ccgttacgtc gacgggaacg gtcggtgcgc
     1561 tcagcgcggg cggcatcgcc gggaacttgt gcatggtgat caacagttac aattccggcc
     1621 gcatcagcgg cgcctcgggc gcgggaacca cgctctacct cggtggcctc ggcggcagcg
     1681 atgtgtatgt gcagaacggc tacaacatcg gcgcggtcag cgcaaaagga tccgggacca
     1741 actttacggg aggcgtaatc gggcatgccg gcgaagcgat atggggagag gtggataccg
     1801 cgctgcatgc ttctttgaac ggcttctggc tgaagcagtc gaaatccggc ggcatcaaca
     1861 gcgacatcaa gtacgccaag ggttcgtatt tttacaaaaa gaaaagcgaa ataaaaaaag
     1921 cgaacagcgg gatcggcacg ctcatcgact cctttgacaa ggaagatccg aacacaatga
     1981 tcgaggcttc atacggcgcg gtctactcat tcgatggtcc gtccgcatcg gttatgacgc
     2041 gatctgatga cgcgaagggg aaaagatcca atctgaacgg tacgctgctt gataatctca
     2101 accggatggt ggaagataga atcaatcgcc tgtaccgccg atgggtcgtt gacgggacca
     2161 atggcggata cccggtgttg tcacccaagc cgactgtgtt cagccatacc tctgaaaaac
     2221 cggacgcatc ggtcgcacag cggatagccg gcgagtattg gggcgtgcac aaacaatgga
     2281 acgacagagt ctttctaggc gcggatggtt ccttcaggcg cgccaagggc ggtgatggcg
     2341 gcgcctggtc atttaccggg aagaggatta tactcaggtg ggccaaatgg gagcctgaga
     2401 ttctcgagca gaagtctgcg ggagcgttct cctctaatgt gtataatttt acgctgaaac
     2461 gcacaggcgt cgcttcccct gacgcgtctg tcccgaccga taaacccgta tcgccgctgg
     2521 cgaagaagct ggcgggagtg tattacgccc agcacaagga ctggaccagt agcctcacca
     2581 tcaatgccaa cggctccttt acgcgtgaca gcaacggcga tagggggacc tggacctatg
     2641 atggaaaaac gcttgttctc acgtgggaca aaggggcgtc cgatacgctc aagaaaacgg
     2701 acacgggatt ctattgtccc gcatataagt tcacgctgcg gcaacgctga cgcgtgccgt
     2761 gaatataaag tatcggtttt acgacagttc atttgcattc ggcacaaaaa cagcaacaat
     2821 aaatatgtac gtgcgtgctc tacggtcttt ttaaagagaa aaagagggca gggcgaaagt
     2881 ttcaattatg aagtgggtgc gatcccttga tcattcaacg gagaaagaaa agatttctca
     2941 gcgccttgtg catcctcacg gggatgacgc cgaagtcctc cgcgccgcgc tcctttaaaa
     3001 gcgcgatcgt tttttcatcg cccgacagga tggcctgccg caggggcgtg gtgaagagtt
     3061 cgccccgggg gttgacttcc gcgccgtttt ctaatagcag tgcggcgatc cggcttcggc
     3121 cgccccaccg tgccgccatg tgaagcggcg tgttgccgct atggtccctg gcgtttacat
     3181 gtgcgccatt acggaccaaa acggcggccc gttcgaaggt tccccacatg gcggcctggt
     3241 gcagcggtgt ttgcccggca ttgtttttcg cgttaacatc ggcgccgttg cggagcagag
     3301 accgtatcat ttcatcgtca tgacgttcgg tgccaaagcg caggaaaaga aacagcggcg
     3361 tatcgccgtc gcgattacgt gcgttgatgt ccgcgccatg gtcgaggaga agcagcatca
     3421 tcctcccgtc gttgttgata acggcccagt gtatcgcctg ttttccggca taatcgcatt
     3481 gatcgatcct gaagccgttc cggagcgcct ttctgacgtt ttcatagtcc gattttttca
     3541 catacgtaaa gaagtactcg tatttgaatg cgctcgcgtc agtcgtcggg cctgaagagc
     3601 cgcaggcggc tgcgatgatg attgaaagtg acaatgcggc catgcggagc gcgtgtttca
     3661 tcgggatgaa cgatgttgtt gctcgcgtgt cgttggtgtc aaatccggtt ttcgatccgg
     3721 agtgcaggga ttctttcaaa atgggtttaa gatgatccga tctcccggat tgtgtcctcc
     3781 gccgatcgat cgtcggtcca gatgccgtgt atggggtcct tgagttcaat cggagtgatt
     3841 tccacctcgg tgtcgtctcg gagcttgacg tggggatcga tgacgatcgt attccctttc
     3901 actattcccc gataagtcat tgtcgcatcc tgttttttcc tgtgatgaaa tcattgtata
     3961 atgcggattg cgctttgtca aatgtaaatg atcttccatg cgccctgggc ttgccccgga
     4021 aaagcggaca cgaagttaag cacaaagtac gatatta
//
LOCUS       DAORHF010000309         4032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_24187_length_4032_cov_5.305255, whole genome shotgun sequence.
ACCESSION   DAORHF010000309 DAORHF010000000
VERSION     DAORHF010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24187_length_4032_cov_5.305255"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="PKJ16_17310"
     CDS             complement(<1..983)
                     /locus_tag="PKJ16_17310"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48805.1"
                     /translation="MRSMKGEMVDTRSFARGRFLLRFLDPVMERQYGEYFAARFLVQA
                     RWSYFLGTLLYVGFLFIDYHVMPESFTRQAIIRCGVVAPISMLIYLLSYTKYYTTFLQ
                     FAVVLVGAAGHYAMAIFSETPPFYVLGTTLILLPFPYLFAGIRFRHALLCGSLIVIGY
                     ELTEVFVTRISAMQLLYYNFFLLSVNGVGIFASHVAERLEGETFVQKLIIADEQKKSE
                     WLLAEVEAKNRSLEESEERYRDIIQNATDGIFKNDSRGRFLFVNPAGASLLGYTSDEL
                     AGMNYRDLLPPDARIVEWMRNERNQNLRRWMVIGEFPNWDRCSGHNHAYPP"
     gene            complement(984..>4032)
                     /locus_tag="PKJ16_17315"
     CDS             complement(984..>4032)
                     /locus_tag="PKJ16_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48806.1"
                     /translation="RAPEPAAPLATIAAPAITTDDDPGVDAEARIANLKEELRAQADY
                     LEINRHELEVANETMRRSEEQFRDIIERATEGIHVANRNGDIQYMNPPGLKKLGYTLD
                     EIVGKNYLELVREDFRDTAALFYLDQMRNRTEDTYLECPVVRKDGVNIWIGMSVKMFT
                     EKQGDIGFYCIARDITEQKKMEDALRESEERYRTLVENASDVIYHCDWRGYFTYFSPY
                     AFRLSGYQPEEILDHHFTSIIHADYRKQMLEFYVQQFRDRVPETYAELPMIKKDGSVF
                     WVGQRVKIVTGSDGKVEFYGIARDVTELKNTENALRASEEKYRTILETNKAGFFEVDL
                     AGSIISCNDVVVSFTKYPREQLIGMSFTKLMSPATADRVFEVYHTVYAREVKVGFVDH
                     EIINSAGEIGFIETTVTLITDEHDAPTGFRALAVDVTARTNAEAALRASEVKYRGILS
                     TIKEGYYELDLKGNFTFINDSMCEIWGRTSEEMIGLNYREYMDERGKDIARDAFRAIY
                     ESRERGTLVDFVIYRKNGDERLLETSLGVLIDSTDAIVGFSGMVRDVTERKKVEDALR
                     VSEQKYRSILENIEDAYYEVNLDGIVIFINDAMCRFMNCPADEICGKSYRAFTDDESA
                     TRIFDIFNSVFMTGAPSESFDYPIIRKGGERLFTESRASLITDARGRAVGFRGILRDI
                     TARKRVAEELQAAKEAAEAANQAKSRFLANMSHELRTPLNAINGIIDLLRFGSFERDE
                     DILASLERMAGRLDLLPADERGEFATFLRDELHSLIDHVTDDGNYKRFVFRRIRSQML
                     SMSPEPDAELAAILDAIESHVDAEEKEVFASYRRIKESGEYLLGLIDTVLNLAKIESG
                     KIEIKKAAVVVREMVDSVLTNARSYAKSKGKEYVRIICTVRDSVPEIAIIDSLKTRQV
                     LLNMFSNAIKFTPRGDVSLHVELDGDRIVFKVSDSGIGIRDEDRGRIFVEFERTESAR
                     NIEGTGLGLAISKRLVELQGGEVGFESEYGKGSTFWFSLPVGG"
BASE COUNT          819 a         1257 c         1096 g          860 t
ORIGIN      
        1 tcgggcggat aggcgtggtt gtggccggag cagcggtccc agttggggaa ttcgccgatg
       61 accatccacc ggcgcaggtt ctggttgcgc tcgttgcgca tccattccac gatgcgcgca
      121 tcgggcggca gcaggtcccg gtaattcatc cccgcaagct cgtcggaggt ataccccagc
      181 agcgatgcgc cggcaggatt gacgaacaga aacctccccc gggaatcgtt cttaaagatg
      241 ccgtccgtcg cgttctgaat aatgtcgcga taccgctcct ccgattcctc gaggctccga
      301 ttcttcgctt caacttccgc caggagccat tcggacttct tctgctcgtc ggcgatgatg
      361 agcttttgga caaacgtttc gccctcgagc cgttccgcga catgtgaggc gaaaatcccc
      421 acgccgttca ccgacagcag gaagaagtta tagtagagga gctgcatggc cgatatccga
      481 gtcacaaaca cctccgtgag ctcgtacccg atgacaataa gcgatccgca cagcagggcg
      541 tgccgaaagc gtatgccggc aaatagatag ggaaagggca gaaggatgag cgtggtgccg
      601 aggacgtaaa atggcggcgt ttccgaaaaa atcgccattg cataatggcc cgcggcgccg
      661 accagcacaa cggcaaactg cagaaaggtc gtgtagtatt tcgtataact caacagataa
      721 atgagcatgc tgatgggcgc caccacgccg catcggatga tcgcctggcg cgtaaagctc
      781 tcgggcataa cgtgataatc aataaacaga aaaccgacat agaggagcgt gccaaggaag
      841 taggaccatc gggcctgcac gagaaagcgc gcggcgaaat actcgccata ctgccgctcc
      901 atgacgggat caagaaacct gagcagaaac cgccccctgg cgaacgatcg cgtatccacc
      961 atctccccct tcatgcttcg cattcaacct cccaccggca gcgagaacca gaaggtgctt
     1021 cccttcccgt actcggactc gaacccgacc tcgccgccct gaagctcgac cagccgtttc
     1081 gatatggcaa gtcccaggcc cgtcccctcg atgttgcggg cactctccgt acgctcgaat
     1141 tccacgaaga tccgtcctcg atcctcgtcc cggatcccga tcccggagtc gctgaccttg
     1201 aagacgatcc gatcgccgtc gagctccaca tgcagcgaca catcgcctct cggagtgaac
     1261 ttgatggcgt tcgagaacat gttcaggagc acctggcggg ttttcagact gtcgatgatc
     1321 gcgatctccg gcaccgaatc ccgcacggtg cagatgatgc gaacgtactc cttgcccttg
     1381 gatttcgcat agctgcgggc gttcgtgagc accgaatcga ccatctcccg gacgacgacc
     1441 gccgccttct tgatctcaat ctttcccgat tcgatcttcg caaggttgag cacggtgtcg
     1501 atgagtccca ggaggtactc gccgctctcc ttgatccggc gatacgacgc gaacacctcc
     1561 ttctcctcgg cgtcgacatg gctctcgatg gcgtcgagga tcgccgcaag ttccgcatcg
     1621 ggctccgggc tcatcgacag catctggctc cggattcttc ggaagacgaa ccgcttgtag
     1681 tttccgtcgt cggtgacatg gtcgatcagc gagtgcagtt catcgcggag gaaggttgcg
     1741 aactcgcccc gctcatccgc cggcaacaga tcgaggcgcc ccgccatacg ctccaatgaa
     1801 gcgaggatgt cctcgtcgcg ctcgaacgag ccgaagcgca gaagatcgat gatcccgttg
     1861 atcgcgttga gcggggttcg cagctcgtgg ctcatgttcg ccaggaaccg gctcttcgcc
     1921 tggttcgccg cttcggccgc ctccttggcg gcctggagct cctccgcgac ccgtttccgt
     1981 gcggtgatgt cgcggaggat gccgcgaaaa ccgaccgccc gacccctcgc gtcggtgatg
     2041 agggacgccc gggactcggt aaacagacgc tcgccgccct tccggatgat cgggtaatcg
     2101 aacgattccg acggcgcacc cgtcatgaag accgagttga agatgtcgaa gatgcgcgtg
     2161 gcgctttcat catccgtgaa cgcacggtag ctttttccgc agatctcatc cgcagggcag
     2221 ttcatgaacc ggcacatggc atcgttgata aaaatcacga tgccgtcgag attcacctcg
     2281 taatacgcat cctcgatatt ctcgagaatg gagcgatatt tctgttcgga aacgcggagt
     2341 gcgtcctcaa cttttttccg ctcggtgacg tctcgaacca tgccgctgaa cccgacaatc
     2401 gcatcagtgg aatcgatgag aacgccaagg gacgtttcaa gcaaccgctc gtcgccattt
     2461 ttacgataga tgacaaagtc caccagcgtt cctctctcgc ggctctcgta aatggcccga
     2521 aaggcatcgc gggcgatgtc cttcccgcgc tcgtccatat actcgcgata gttgagaccg
     2581 atcatctcct cgctggtgcg tccccatatc tcgcacattg aatcgttaat aaacgtaaag
     2641 ttgcccttta gatcgagctc gtaatatccc tccttaatgg tggagagtat cccccggtat
     2701 ttcacctcgc ttgcgcgaag ggccgcctcg gcattcgtcc gcgcggtaac atccaccgcc
     2761 agggcccgga aacccgtcgg agcgtcatgc tcgtcagtga tcagcgtcac cgtcgtttcg
     2821 atgaaaccga tctcgcccgc gctgttgatg atctcatggt ccacgaatcc gaccttgacc
     2881 tcacgggcgt acaccgtgtg atacacttca aaaacccggt cggccgtggc cggcgacatg
     2941 agtttcgtga agctcattcc gatcaactgc tcccgcgggt acttcgtgaa gctgacaacg
     3001 acatcattac aggagatgat cgatcccgca agatcgacct cgaagaagcc ggccttgttg
     3061 gtttcgagga tggtccgata cttctcctcg ctcgcccgaa gcgcgttctc ggtatttttt
     3121 aactccgtaa cgtctcgggc gatcccgtag aattccacct tgccgtcgga tcccgtcacg
     3181 atcttaacgc gctgcccgac ccagaacacc gagccgtcct ttttgatcat cggaagctcg
     3241 gcgtaggtct cgggcacgcg atcccggaac tgctgcacgt aaaactcaag catctgcttc
     3301 cgataatcgg cgtggatgat agacgtgaag tgatgatcga gtatttcctc cggctggtac
     3361 cccgaaagcc ggaacgcgta gggactgaag taggtgaaat acccgcgcca atcgcagtgg
     3421 tagatgacat cgctcgcatt ctcgaccagc gtccggtatc gctcctcgct ctcgcgcagc
     3481 gcatcctcca tctttttttg ctcggtgatg tcacgagcga tacaatagaa gccgatatcg
     3541 ccctgttttt cggtgaacat cttcacgctc atgccgatcc aaatgttcac tccgtccttc
     3601 ctgacgaccg ggcactcgag gtaggtgtct tcggtcctat tccgcatctg atccagatag
     3661 aagagcgccg ccgtgtcccg aaaatcctcc cgcaccagct cgagatagtt tttccccacg
     3721 atttcatcaa gcgtgtaccc cagcttcttg aggcccggag ggttcatata ctggatatcg
     3781 ccgttcctgt tggcgacatg gatcccttcg gtcgccctct cgataatatc ccggaactgc
     3841 tcctccgatc gccgcattgt ctcgttggcg acctccagct cgtgccggtt gatctccagg
     3901 tagtccgcct gggcccgcag ctcctccttc aggttggcga tgcgcgcctc ggcatcgacg
     3961 cctggatcgt cgtcggtcgt gatcgccggg gcggcgatcg tcgcgagcgg tgcggcgggt
     4021 tcgggggcgc gc
//
LOCUS       DAORHF010000310         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_24474_length_4002_cov_5.498860, whole genome shotgun sequence.
ACCESSION   DAORHF010000310 DAORHF010000000
VERSION     DAORHF010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24474_length_4002_cov_5.498860"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /gene="dnaK"
                     /locus_tag="PKJ16_17320"
     CDS             <1..1488
                     /gene="dnaK"
                     /locus_tag="PKJ16_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HNV48807.1"
                     /translation="GRIAGLTVERIINEPTAASLAYGLDKKTKNEKIAVYDLGGGTFD
                     VSILELGDGVFEVKSTNGNTHLGGDDFDQRIMNWLIDEFKKQTGIDVSSDKMALQRLK
                     EGAEKAKIELSGKSQTEINIPFLTADQSGPKHLIMTLTRAKFEQLVEDLVESTKIPCQ
                     RALEDAGLTPGDIDEVILVGGSTRIPAVQELVRRIFGKEPHKGVNPDEVVAVGAAIQG
                     GVLAGDVHDVLLLDVTPLSLGIETLGGVMTKLIERNTTIPTRKSQVFSTASDSQPAVS
                     IHVLQGEREMAAQNRTLGRFDLVGIPPAPRGVPQIEVTFDIDANGIVHVSAKDLGTGK
                     EQKIRIESSSGLNEEEIKNMVREAETHADEDKKLRELIETRNSADTLIYSIEKLLREN
                     EGKIEAAEKQAIEAEVENLKSAMGADDVEQIKAAKTKLEQASHKFAERIYRDAAAQQG
                     GAEQGAPGAGANPGAGQDNKASGAGPGQGEKVYDADYEVVDDDKK"
     gene            1504..2613
                     /gene="dnaJ"
                     /locus_tag="PKJ16_17325"
     CDS             1504..2613
                     /gene="dnaJ"
                     /locus_tag="PKJ16_17325"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HNV48808.1"
                     /translation="MANKRDYYEVLGVAKDAGEDQIKQAYRKLALKYHPDKNRGNPEA
                     EEKFKEATEAYEILRDPKKRAQYDKFGHEGVAGFEGFGRGAYTDFSDIFGEFDLGDIF
                     EGFFGSGFGKRGRAGRTRRGHDIQYDMDITLEDAFNGKEVKIDIPRNETCETCGGSGA
                     EAGTKPAVCNVCSGTGQIRRTQGFFTIAQTCYRCGGAGKMITSPCKACGGGGSIVKNR
                     KITVKVPAGVESGSRLKINGEGEMGENGGPRGDLYVVLHIMKHEIFERHGNDIINIVD
                     ISFPMACLGGDIEVPTIGGKKAKMKIPPGTDNGHIFRLKGNGIPYLGSYGRGDQLVKI
                     NIHVPKKLTPRQKELLQEFAKLDSEAVGSSTRQFY"
     gene            2615..3403
                     /locus_tag="PKJ16_17330"
     CDS             2615..3403
                     /locus_tag="PKJ16_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48809.1"
                     /translation="MIRLRRSIVAVACGAVIAGIASFAALGYITYAGSGGDVVTPATY
                     PNRHHFTQAIFWFELLDSREELFEALGDPGTPQGAAMRRIMDVTNTYDVLLFSAYPPF
                     FALLLALVHLLLRRDDADPPAALPILVAGIVVCAVMPLADHMENVQLLKLTTFAAPGD
                     VDDGVIAALKVWTRIKWGAIFAVTALLALRYWRYFIDSRFPRYAFTGIYGITAVVGAI
                     SLMFDGTRFLIEAASTTMGIGWLVSAVHAGVVAVKAMRMRERAE"
     gene            3580..3894
                     /locus_tag="PKJ16_17335"
     CDS             3580..3894
                     /locus_tag="PKJ16_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48810.1"
                     /translation="MIRPTIFLAITCLFFMILSLSIHDAAYRVGTPGFAACTTEEIKN
                     KCCYEFLELMKSELETDQRTTREDRAVRGYVRCLRSVNCSQEMIEMKAKNVRQIRSIC
                     GE"
BASE COUNT          804 a         1230 c         1304 g          664 t
ORIGIN      
        1 gggcgcatcg cgggcctcac cgtcgagcgc atcatcaacg agcccacggc ggcgtcgctc
       61 gcctacggcc tggacaagaa gacgaagaac gagaagatcg cggtgtacga cctcggcggc
      121 ggcaccttcg acgtctcgat cctcgagctc ggcgacggcg tattcgaggt gaaatcgacc
      181 aacgggaaca cccacctggg cggggacgat ttcgaccagc gcatcatgaa ctggctcatc
      241 gacgagttca agaagcagac cggcattgac gtgtccagcg acaagatggc gctccagcgg
      301 ctcaaggaag gcgccgagaa ggccaagatc gagctttcgg gcaagtcgca gacggaaatc
      361 aacatcccgt tcctgacggc ggaccagagc gggcccaagc acctcatcat gacgctgacc
      421 cgcgccaagt tcgagcagct cgtcgaggac ctggtcgagt cgaccaagat cccgtgccag
      481 cgcgcgctgg aggacgcggg actcacgccg ggcgatatcg acgaggtgat cctggtgggc
      541 ggatcgacgc ggatccccgc ggtccaggag ctcgtgcgcc gcatcttcgg caaggagccg
      601 cacaagggcg tgaaccccga cgaggtggtc gccgtgggcg ccgcgatcca gggcggcgtg
      661 ctcgcgggcg acgtgcacga cgtgctcctg ctcgacgtga ccccgctctc gctggggatc
      721 gagaccctgg gcggcgtgat gaccaagctc atcgagcgca acaccaccat cccgacgcgc
      781 aagagccagg tgttctccac cgcgtcggac agccagcccg ccgtgtccat ccacgtattg
      841 cagggcgagc gcgagatggc cgcgcagaac cggacgctgg gacgcttcga cctggtgggc
      901 atcccgcccg caccgcgcgg cgtgccgcag atcgaggtga ccttcgacat cgacgcgaac
      961 ggcatcgtgc acgtttcggc gaaggacctg gggaccggca aggagcagaa gatccgcatc
     1021 gagtcctcga gcggcctcaa cgaggaggag atcaagaaca tggtgcgcga agccgagacg
     1081 cacgcggacg aggacaaaaa actccgcgag ctcatcgaga cgcgcaacag cgccgacacg
     1141 ctgatctact ccatcgaaaa gctcttgcgg gagaacgagg ggaagatcga agcggccgaa
     1201 aagcaggcga tcgaggccga ggtcgaaaac ctcaagagcg cgatgggagc ggacgacgtc
     1261 gagcagatca aggcggccaa aaccaagctc gagcaggcgt cgcacaagtt cgccgagcgc
     1321 atctatcgtg acgccgcggc gcaacagggc ggcgcggagc agggcgcgcc gggcgccggc
     1381 gcgaacccgg gcgcgggcca ggacaacaag gcctccggtg cgggaccggg ccagggagag
     1441 aaggtgtacg acgccgatta cgaagtggtc gacgacgata aaaagtaatc gcgagaggat
     1501 gctttggcca acaagagaga ttactacgag gtgctgggcg tcgccaagga cgcgggtgag
     1561 gaccagatca agcaggccta tcgcaagctt gccctgaagt atcacccgga taagaaccgc
     1621 gggaatcccg aggccgagga gaagttcaag gaagcgaccg aggcctacga gatcctgcgc
     1681 gatcccaaga agcgcgccca gtacgacaag ttcggccacg agggcgtcgc cgggttcgag
     1741 ggattcggcc gcggcgccta caccgatttc tccgacatct tcggtgagtt cgacttgggc
     1801 gacatcttcg aggggttctt cgggagcggg ttcggcaagc gcggccgtgc gggacgcacg
     1861 cgtcgcgggc acgatattca gtacgacatg gatatcaccc tcgaggacgc gttcaacggg
     1921 aaggaggtca agatcgacat cccccgtaac gagacctgtg agacctgcgg cgggtccggc
     1981 gccgaggccg gcaccaagcc ggccgtctgc aatgtgtgca gcggcaccgg gcagatccgc
     2041 cgcacgcagg gattcttcac catcgcgcaa acctgttacc ggtgcggcgg cgcgggcaag
     2101 atgatcacct cgccctgcaa ggcctgcggc ggcggcggct ccattgtgaa gaaccgcaag
     2161 atcaccgtga aggtcccggc cggcgtcgag tcgggatcgc ggctcaagat caacggcgag
     2221 ggcgagatgg gcgagaacgg cgggccgcgc ggcgacctgt acgtggtcct gcacatcatg
     2281 aagcacgaga tcttcgagcg tcacggcaac gacatcatca acatcgtgga catctcgttc
     2341 ccgatggcgt gcctgggcgg cgatatcgag gttcccacga tcggcggcaa gaaggccaag
     2401 atgaagatcc cgcccgggac cgacaacggc cacatcttcc gcctcaaggg gaacgggatc
     2461 ccgtatctgg gctcctacgg gcggggcgac cagctcgtga agatcaacat ccacgttccc
     2521 aaaaagctca cgccgcggca gaaggagctc ctgcaggagt tcgcgaagct cgacagcgag
     2581 gcggtgggat cgtcaacgcg gcagttttat taggatgatt cgccttcgcc ggagcatcgt
     2641 cgcggtcgcg tgtggcgccg tgatcgcggg catcgcctcg tttgccgcgc tcggctatat
     2701 cacctacgcc gggagcggcg gcgatgtcgt cacgccggcg acctatccca accggcacca
     2761 tttcacccag gccatcttct ggttcgagct cctcgacagc cgggaggagc tcttcgaggc
     2821 gctcggcgat ccgggcacgc cgcagggcgc ggccatgcgg cgcatcatgg acgtcacgaa
     2881 cacctacgat gtgttgctct tttcggccta cccgccgttc tttgcgctgc tgcttgcgct
     2941 ggtccacctc ctgctgcgaa gggatgacgc ggacccgccc gccgcccttc cgatcctcgt
     3001 cgcggggatc gtcgtctgcg ccgtgatgcc gctcgcggat cacatggaga acgtgcagct
     3061 cctgaagctc acgacatttg ccgcccccgg cgatgtcgat gacggcgtga tcgccgcgct
     3121 caaggtgtgg acgcggatca agtggggcgc gatcttcgcc gttacggcgc tcctggcgct
     3181 tcgctattgg cggtatttca tcgattcacg gttcccgcga tatgcattta ccggcatata
     3241 cggcataacg gcggtcgtgg gtgcgatatc cctcatgttc gacgggacgc gcttcctgat
     3301 cgaggcggcc agcaccacca tgggcatcgg ctggcttgtg tcggccgtgc acgcgggcgt
     3361 ggttgccgtg aaggcgatgc gcatgcgcga acgcgcggaa taattcctcc ccatctcgca
     3421 ccccggttat cgacatagtg cgttttgtcc gcgtatgatg tcttgttgtg taactccatg
     3481 acaggcaacc cgggtttgcg tcaacgtaaa tgaatacggt tgacgctgat ctgcgccgaa
     3541 taataatcag tgacgttact ttgtgcgctg ggggcttcca tgatcagacc gacgatattt
     3601 ctcgccataa cctgcctgtt tttcatgatc ctgtcgcttt ctatccatga cgccgcgtac
     3661 cgggtgggga ctcccggctt tgccgcatgc acgaccgagg agatcaagaa caagtgctgc
     3721 tacgaattcc tcgagctcat gaagagcgaa ctggagaccg accagcggac cacacgcgag
     3781 gaccgggccg tgcgcggata tgtccggtgc ctgcgatcgg tcaactgctc ccaggagatg
     3841 atcgagatga aggctaaaaa cgtacggcag atccgctcga tctgcggcga gtagttttca
     3901 ctttctatcg accggttttt ttcgacttct ttttcgcttt cttggtgtcg cgctctgccg
     3961 cccggggcca tagcgcaatg ggatcctcga tcatcatgag cg
//
LOCUS       DAORHF010000311         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_24854_length_3958_cov_4.917499, whole genome shotgun sequence.
ACCESSION   DAORHF010000311 DAORHF010000000
VERSION     DAORHF010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24854_length_3958_cov_4.917499"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="fmt"
                     /locus_tag="PKJ16_17340"
     CDS             <1..668
                     /gene="fmt"
                     /locus_tag="PKJ16_17340"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HNV48811.1"
                     /translation="PRAIFDLPRHRTINLHPSLLPRYRGAAPMQWALINGETETGITV
                     QLINERLDAGDIILQERFAIDETMTAGELAEIVFPRGAALLDEAIVRIAAGTAAPVVQ
                     DESAATYCKKIDKDLPRIVWTGGARDIHNMVRGLNPAPVAWTTFRGRVLKIWRTMPFE
                     AADAPALDPGEVAVYKKKRLLAGTGVGRLEIVSVQPETKKVMDAASFINGHRLQAGER
                     LGV"
     gene            781..1056
                     /locus_tag="PKJ16_17345"
     CDS             781..1056
                     /locus_tag="PKJ16_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014958954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HNV48812.1"
                     /translation="MFEFDSGLSQEKRKARDLRASAWWRKKTAAGICHYCRRQCKPGE
                     LTMDHIIPLSRGGTSERFNIVPACKECNNRKKYLLPTEWDEYLNSIT"
     gene            1077..3536
                     /locus_tag="PKJ16_17350"
     CDS             1077..3536
                     /locus_tag="PKJ16_17350"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HNV48813.1"
                     /translation="MAEIASGKNAETLRKGAFLVVQGDEPRFVHFLEKGSLEILSAPK
                     EFNGKDADTIIAYSRRVGTISGRSLVSGLSLVFSQPYTKSIRALEDSVVTKYPIREGG
                     FRQMIDENLSLALGVLDNLVKRIDLSLTDAAKYMKLYQNLCKISDNIAIMASSIGDHK
                     LPEGLLNHADELYGTFTEHGGELPLSFDGKFLVSDNSAALRKKYVFPGLPLESIADLK
                     QCSFFKRLMGAGQEIVKSSMRSDPNIATYIFETASDNLIKILDRIENVHKEIDEQIAH
                     LFGEEESWAMYLADGKGLDRWASTGKLSPDFLKNLLTVAMKINEFYEEISGEKIVENY
                     PGLKRLHELYQSRKDSGGEAAPAQQRQAAIAPSIGKEYKNSIHQIFEFALFDKENQKI
                     LLQALKTFKEMKNPFNTEIDGRKVRRTITKAYWDLYKQAYIRSRSEGVAPLPVRQMIR
                     FGFLDETLVEPQQLVELHELINRHVERTEIPIHLEEEFLMRVNTGQDIPSINEMGLTY
                     EQHLKEEGKAPTPKDKKKAPDVDDGVKKTFYEIEQRVQRTVAVCSGSTATAFPVLTAM
                     VVKTGLTNLYVSKRKLESVIKELRDVDYSVFYREASIDLGERRELIKQEVVPNFIILP
                     AFGSKTMMWQDLEGINRKSRGRIVVPILFMGDLIKAMAHTFAVFRYELNRTITGAMWR
                     DPVEGGITGIYLDYVQFFKKNPNLSIEAKEKIKEKFKSLRDDRSKFAYDYMLWVLFEK
                     DGTPKLNNVVRDIFYKNIPFKKEVRTKLETMPAFAETAVRFKNVNTRTVEGYKRKFKK
                     YLDDPGNMPEELKRFMDFLNT"
     gene            3565..>3958
                     /locus_tag="PKJ16_17355"
     CDS             3565..>3958
                     /locus_tag="PKJ16_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48814.1"
                     /translation="MGNNKKKVTGIIRVYSRKARDQIVGIGSEFFNEAGIADCAEDVF
                     ACVDELIKNAVKANYKFLLIMDRIQEDLIRDNPGRDADDIKRQTYDIIKDKKMFDRIA
                     SRIVSSEEISQTVREILNQESQQLSIKNR"
BASE COUNT          867 a         1206 c         1209 g          676 t
ORIGIN      
        1 tcccccgcgc gatcttcgat ctgccgcggc accgcaccat caacctgcac ccgtcgctct
       61 tgccgcgcta ccgcggcgcg gcgcccatgc agtgggcgct catcaacggc gaaaccgaga
      121 cggggatcac ggtgcagctc atcaatgagc ggttggacgc gggcgatatc attctgcagg
      181 agcgcttcgc gatcgacgaa accatgaccg cgggcgagct cgccgagatc gtgttccccc
      241 gcggcgcggc gctcctcgac gaggccatcg tccgtatcgc cgcgggcacc gccgcgcccg
      301 tcgtccagga cgagtccgcg gccacctact gtaaaaaaat cgacaaggat ctgccgcgga
      361 tcgtgtggac cggcggcgcg cgcgacatcc ataacatggt tcgcgggctc aatcccgcgc
      421 ccgtcgcctg gacgaccttc cgcggccgcg ttctaaagat ttggcgcacc atgccgttcg
      481 aggcggcgga cgcccccgcg ctcgatccgg gcgaggtcgc ggtgtacaaa aagaagcgtt
      541 tgttggccgg gaccggcgtg gggcggcttg agatcgtgtc agtgcagccc gagacgaaga
      601 aggtgatgga cgccgccagc ttcatcaacg gacaccggct gcaggcgggc gagcgtttgg
      661 gcgtgtaacg cctgcgctat tttttattga caccgcgggg cgtgtgcact agcgttatca
      721 tactccaatt gtacaggaca taacgcggtc atcatgcccc ggcggacgtc agcccatagc
      781 gtgttcgagt ttgattcggg cctttcgcag gaaaagcgaa aggcgcgcga ccttcgcgca
      841 tcggcgtggt ggcggaaaaa gactgccgcc ggaatctgcc actactgtcg gcggcagtgc
      901 aagccgggcg agttgaccat ggaccacatc atcccgctgt cgcgcggggg cacctcggag
      961 cggttcaata tcgtgcctgc ctgcaaggag tgcaataatc ggaaaaagta tctcctcccg
     1021 accgagtggg acgagtatct caattcaatc acgtaggcga cggtacgggc gggaccatgg
     1081 ctgagatcgc atcgggaaaa aacgccgaga cgctccgcaa gggggcgttc ctggtcgtgc
     1141 agggcgacga gccgcggttt gtccactttc tggagaaggg gtcgcttgag atcctctcgg
     1201 cgcccaagga gttcaacggc aaggacgccg acactatcat cgcctacagc cgccgagtgg
     1261 gcaccatcag cgggcgctcg ctggtgtccg gcttgagtct cgtcttcagc cagccctaca
     1321 ccaagtcgat ccgggcgctc gaggattcgg tagtgacgaa ataccccatc cgcgagggcg
     1381 gcttccgcca gatgatcgac gagaacctct cgctcgccct gggggtgctc gataacctcg
     1441 tcaagcggat cgacctttcc ctcaccgatg ccgccaagta catgaagctc taccagaacc
     1501 tctgcaagat cagcgataac atcgccatta tggccagcag catcggcgac cacaagctcc
     1561 ccgagggcct gctgaaccac gccgacgagc tctacgggac cttcaccgag cacggcggcg
     1621 agctgccgct gtcgttcgac ggcaagttcc tcgtgtccga caacagcgcc gcgctgcgca
     1681 agaagtacgt gttcccgggg ctgcccctcg agtcgatcgc cgacctcaag cagtgctcct
     1741 tcttcaagcg cctgatgggg gcgggacagg agatcgtcaa gtcctccatg cgcagcgacc
     1801 cgaacatcgc cacctatatc ttcgagaccg cgtcggacaa tctcatcaag atactcgacc
     1861 gcatcgagaa cgtgcacaag gagatcgacg agcagatcgc ccacctcttc ggcgaggagg
     1921 agagttgggc catgtatctt gccgacggca aggggctgga ccgatgggcc tccaccggga
     1981 agctctctcc cgattttctg aagaacctcc tcacggtcgc gatgaagatc aacgagttct
     2041 acgaggagat ctccggggag aagatcgtcg agaattatcc cggcctgaag cggctccacg
     2101 agctgtacca gtcgcgcaag gacagcggcg gcgaggccgc tccggcccag cagcgccagg
     2161 ccgccatcgc gccgtcgatc gggaaggagt acaagaattc catccatcag atcttcgagt
     2221 ttgcgctctt cgacaaggaa aatcagaaga tactccttca ggccctcaag accttcaagg
     2281 agatgaagaa ccccttcaac accgagatcg acgggcggaa ggtgcggcgc accatcacca
     2341 aggcctactg ggacctctac aagcaggcct acatcaggag ccgcagcgag ggcgtcgcgc
     2401 cgcttccggt gcgccagatg atccgcttcg gtttcctgga cgagacgctg gtggagccgc
     2461 agcagctggt ggagctgcac gagcttatca accggcacgt cgagcgcacg gagatcccca
     2521 tccatctcga ggaagagttc ctgatgcgcg tgaacaccgg acaggacatc cccagcatca
     2581 atgagatggg actcacctac gagcagcacc tcaaggagga ggggaaggcg cccacgccca
     2641 aggacaagaa gaaggcgccg gacgtcgacg atggagtcaa gaagaccttc tacgagatcg
     2701 agcagcgcgt ccagaggacc gtcgcggtgt gctcgggatc gaccgcgacc gccttcccgg
     2761 tgcttaccgc gatggtggtc aaaaccgggc tcacgaacct gtatgtctcc aagcgcaagc
     2821 tcgagtcggt gatcaaggag ctgcgcgatg tcgattactc cgtgttctac cgcgaggcct
     2881 ccatcgacct cggcgagcgg cgcgagctca tcaaacagga ggtggtgccc aacttcatca
     2941 tcctgcccgc cttcggctcg aagacgatga tgtggcagga cctcgagggc atcaaccgga
     3001 aatcgcgcgg acgcatcgtg gtccccatcc tgttcatggg cgatctcatc aaggcgatgg
     3061 cgcacacctt tgcggtgttc cgctacgagc tcaatcgaac catcaccggc gcaatgtggc
     3121 gcgatcccgt cgagggcggg atcaccggga tctacctcga ttacgtccag tttttcaaga
     3181 aaaacccgaa cctctccatc gaggcgaagg agaagatcaa ggagaagttc aagagcctgc
     3241 gggacgaccg gagcaagttc gcctacgatt acatgctctg ggtgctcttc gagaaggacg
     3301 gcaccccgaa gctcaacaac gtggtgcgcg atatctttta taaaaacata ccgttcaaga
     3361 aggaggtccg gacgaagctg gagaccatgc cggcctttgc cgaaacggcc gtccgcttca
     3421 agaacgtgaa cacccgcacg gtcgaggggt acaagcggaa attcaagaag tatctcgacg
     3481 atcccggcaa catgccggag gagttgaagc ggttcatgga ctttctcaat acctaggatt
     3541 cgtcggggcg gggtgcgata gcgcatgggc aacaacaaga aaaaggtaac ggggatcatc
     3601 cgggtgtaca gccgaaaggc gcgcgaccag atcgtgggca tcggctccga gtttttcaac
     3661 gaggccggca tcgccgattg cgcggaggac gtgttcgcgt gcgtggacga gctgatcaag
     3721 aacgcggtga aggcgaacta caagtttttg ctcatcatgg accgcattca ggaggacctg
     3781 atccgcgaca atccgggccg cgacgcggat gatatcaagc ggcagacata cgatatcatc
     3841 aaggacaaga agatgttcga ccgcatcgcc tcgcggatcg tttcgagcga ggagatttcg
     3901 cagacggtgc gcgagatcct gaaccaggag tcgcagcagc tctccatcaa gaaccgct
//
LOCUS       DAORHF010000312         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25055_length_3937_cov_4.339773, whole genome shotgun sequence.
ACCESSION   DAORHF010000312 DAORHF010000000
VERSION     DAORHF010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25055_length_3937_cov_4.339773"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PKJ16_17360"
     CDS             complement(<1..491)
                     /locus_tag="PKJ16_17360"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HNV48815.1"
                     /translation="MSEKKTTAVDAYVGAQFRSWEQNKQIVQSDRERLVVDRVPMPFV
                     TISREYGCGGFEIAERVVTIINEELSPPRVWAAYDRKILERVVSDMGLSERLAETLSN
                     TARTTMNSFIGHALGGLPSQVEVYRRLVEIVRTLAANGHVIVVGRAGNAITRDMENGL
                     HVK"
     gene            complement(525..1379)
                     /gene="asd"
                     /locus_tag="PKJ16_17365"
     CDS             complement(525..1379)
                     /gene="asd"
                     /locus_tag="PKJ16_17365"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HNV48816.1"
                     /translation="MRKKSLTIFLFKVLPTGLISRVFGVIARMPLPGFLLRRVIDWYI
                     RSYGVDRDEIDWPQRGFRSLDAFFTRTLVPGTHMIDADPRSVVSPVDARIDMFGEIID
                     GVTMMQAKGVCYSLRDLVPSAMHERFIDGSFVTLYLSPSDYHRIHAPAGGRVRGFFNI
                     PGRLFTVQEFMVQGLPGLFSRNERLISYIETQRGLVAVCKIGAMNVGRITLSYDPIAT
                     NRTFRRRREVLFGDDAACPIAKGDELGVFHLGSTVIMLFEKDMITFTDCAVGTKVRVG
                     QRLAEFRA"
     gene            complement(1345..2793)
                     /locus_tag="PKJ16_17370"
     CDS             complement(1345..2793)
                     /locus_tag="PKJ16_17370"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HNV48817.1"
                     /translation="MRSVHVVGICGVAMGTLAGMLKERGVSVTGSDENVYPPMSDMLR
                     SWGIPVHQGYDAGRIGSPDLVIIGNVISRGNPEAEAVLNRRIPYLSMAQAIQRFFLCE
                     KETIAVCGTHGKTTTTCLLAHIFETAGRDPSFLVGGVSRNYNSNYRMGRGAHFIIEGD
                     EYDSAFFEKVPKFIVYRPQHIVLTSLEFDHADIYRDLDEISLWFSRLINIIPSDGTIV
                     YSKEYPILDTLCASSFSRVRSYGPTSADVAYRFLRYDGERAVITLESRDAGEFELRTT
                     VSGSFNYSNIAAAASTALAFGIESAVIIEAVRTFRGVKRRQELIFRAPGIRIYEDFAH
                     HPTAIAGIMREMRQRYPDYTLWALYEPRSATSRRNVFQRELPGAFVSADRVLIKRPPL
                     MEKIPADDRIDIEAVIASLRAMNARADLFESGGAMADHVFDSIDAASKNVLVVMSNGG
                     FDGIYQRLVQLASDRYGISDHDEKKIPDHLLV"
     gene            complement(2835..3242)
                     /locus_tag="PKJ16_17375"
     CDS             complement(2835..3242)
                     /locus_tag="PKJ16_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48818.1"
                     /translation="MHQRKHMSIFCAVLLAFALMSVAVGAEKEKATSPEKKVESKKSA
                     GKKKAVRAKVSGASFYGKIIFLGADGCEVKSGKRELSVAFAPDTVFIAKDGSKKDKAI
                     LELCQMVKVVYARKGKENVLKRIAVVKPGTCAR"
     gene            3604..>3937
                     /locus_tag="PKJ16_17380"
     CDS             3604..>3937
                     /locus_tag="PKJ16_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48819.1"
                     /translation="MTVIAFTVILFAPNAFATLSGLTWGISKSECVQKFSYTDADIRA
                     FSPSDDSRYENRIMNYIVAIDSDLKSDITIFRTVKSPIRDLLFVKNRLYSILEDYGTV
                     SAAVERRTL"
BASE COUNT          878 a         1199 c         1037 g          823 t
ORIGIN      
        1 attttcacgt gcagcccgtt ttccatgtcc cgggtgatcg cgtttccggc gcgtcccacg
       61 acgatcacat gcccgttcgc ggcgagcgtc ctgacaatct cgacgagccg ccgatacacc
      121 tcgacctgcg acggaagacc gccgagggcg tggccgatga agctgttcat ggtggtgcgt
      181 gcggtattgg aaagcgtttc ggccaggcgc tcggagagcc ccatgtcgga aacgacccgc
      241 tcaagaatct tccggtcgta ggccgcccac acgcgcggcg gcgaaagctc ctcgttgatg
      301 atcgtcacga cgcgctcggc gatctcaaaa ccgccgcacc catactcgcg ggatatggtc
      361 acaaagggca tcggcacgcg atcaaccacg agacgctcgc ggtcactctg cacgatctgc
      421 ttgttctgct cccacgaacg gaattgcgcc cccacgtagg cgtctacggc tgtcgttttt
      481 ttttcggaca tggacatcct cccatctggt cgcgcggatg acggttacgc gcggaattcc
      541 gcaaggcgct gccccacccg caccttcgtc cctacggcac aatccgtgaa tgtaatcatg
      601 tccttctcaa agagcatgat gacggtggac ccgagatgga atacgcccag ctcatcgccc
      661 ttggcgatcg gacatgccgc atcgtcgccg aagagcacct cccggcgtcg ccgaaacgtg
      721 cggtttgtcg cgatcggatc gtacgacagg gtgatccgcc ccacgttcat cgcgccgatc
      781 ttgcagacgg cgaccagccc gcgctgcgtt tcaatgtagc tgatgagccg ctcgtttcgg
      841 gagaagagcc ccggcagtcc ctgcaccatg aattcctgca ccgtaaagag cctgccggga
      901 atattgaaaa agccgcgcac gcgccctccg gccggcgcat ggatccggtg ataatccgag
      961 ggcgagagat acagagtgac aaagctccca tcgatgaaac gctcgtgcat cgccgaaggg
     1021 acgagatccc gaagcgaata gcacacaccc ttcgcttgca tcatggtaac gccgtcgatg
     1081 atctccccga acatgtcgat ccgcgcgtcc accggggaga cgacggaccg cggatcggca
     1141 tcgatcatat gcgtgcccgg gacgagcgtt cgcgtaaaaa acgcgtcgag cgagcgaaag
     1201 ccgcgctgcg gccagtcgat ctcgtcacga tccacgccat aggaccgaat gtaccagtcg
     1261 atcacccgcc gcaggagaaa tcccggcaat ggcatccggg cgatgacccc gaagacgcga
     1321 gagatcaggc ccgtcggcag gaccttaaac aagaagatgg tcagggattt ttttctcatc
     1381 gtgatcactg attccgtacc ggtccgaggc caactgcacc agacgctgat atattccgtc
     1441 gaacccgccg ttcgacatga caaccaggac gttctttgac gcggcgtcga tcgaatcgaa
     1501 gacgtggtcc gccatcgctc cgccgctctc gaagaggtcc gcacgagcat tcattgcgcg
     1561 aagcgacgcg atcaccgctt cgatgtcgat tcgatcatcc gccgggatct tctccatcag
     1621 cggcggccgc ttgatgagaa cgcgatcggc tgaaacgaac gccccgggca gctcgcgctg
     1681 gaacacgttg cgcctgctgg tcgccgatct cggttcgtac agcgcccaga gcgtgtagtc
     1741 gggatatcgc tggcgcatct cgcgcataat ccctgcgatt gccgtgggat gatgcgcgaa
     1801 atcctcgtaa atcctgatgc ccggggccct gaagataagc tcctgtcgcc gcttcacgcc
     1861 gcgaaaggtc cgaaccgcct cgataatcac agccgattct attccgaacg caagcgccgt
     1921 cgaggcggcc gcggcgatgt tcgaataatt aaatgatccg ctcacggtcg tacgcaactc
     1981 gaactcgccc gcatcgcgcg actccagcgt tatcaccgca cgctcgccgt cgtaccgcag
     2041 aaaccgatag gcgacatcgg cgctggttgg accatacgag cgaacgcgcg agaacgatga
     2101 cgcgcacagc gtgtccagga tcggatattc cttcgaatac acgatcgtcc catccgacgg
     2161 gattatgttg atgaggcgcg agaaccacaa tgaaatctcg tcgagatcgc gatagatgtc
     2221 ggcgtggtcg aactccagcg aggtgagcac gatatgctgg ggccggtata cgatgaactt
     2281 cggcaccttt tcgaagaagg ccgaatcata ctcgtcgcct tcgatgataa aatgcgcgcc
     2341 gcggcccatt cgataattgg aattgtaatt tcgcgatacg ccgccgacca gaaatgaggg
     2401 atcacgcccg gccgtttcga agatatgcgc cagcagacag gtcgtggtgg tcttcccatg
     2461 cgtgccgcac accgcgatcg tttccttctc gcaaagaaag aagcgctgga tggcctgcgc
     2521 catcgacagg tacggaatgc gtcggttgag gacggcctca gcctcggggt tcccgcggct
     2581 gatgacattg ccgatgataa cgagatcggg cgaaccgatc cgtccggcgt catatccttg
     2641 atggacggga atcccccagg aacgcagcat atcgctcatg ggcggatata cattctcgtc
     2701 ggacccggtg accgacaccc ctcgctcctt aagcatgccc gcaagcgtac ccatggcaac
     2761 gccgcatatc ccgacgacat ggaccgatct caccggtccg tgcgcaccat cgattccaga
     2821 acgctgtgtt tcgctcaccg cgcgcacgtg cccggtttta cgacggcaat tctcttaaga
     2881 acgttttcct tgccctttcg cgcatacacg accttgacca tctggcaaag ttcgaggatc
     2941 gctttgtcct tcttcgatcc gtcctttgcg ataaacacgg tgtccggcgc aaatgcaacc
     3001 gaaagctctc gcttcccgga tttcacctca cagccatcgg cgccgagaaa aatgatcttc
     3061 ccgtaaaacg acgcccccga caccttcgcg cgcaccgctt ttttcttacc ggcgcttttc
     3121 ttcgattcta cttttttctc aggtgacgtg gctttttcct tttccgcacc cacagcgacc
     3181 gacatgaggg cgaacgcgag aagaacggca cagaaaatcg acatatgttt tctttgatgc
     3241 atggcaccac ctccacgatg gaaacgtact caggctggca cgtaaaatca acttctttac
     3301 agaatcaaat aagtaaccat ttcaaggaaa atgtctgttg attctaatat aatggcatat
     3361 ttttatcaat tataaatcta aaatatttga caatattttt tattcaaata catcgtgtgt
     3421 gcgatacggc atgatagccc aacgcaccat tgaacttttt tcgattaaga cccgctcgga
     3481 acaaaggcca ggcgccggca caatctggat cttggacgtt acacgtatac gacaacgcgc
     3541 aactggaatg agtatatgac acgaacatct cacacatgca ccctcaggtc agtcgccatc
     3601 atcatgacgg tgatcgcgtt taccgtgatc ctcttcgcac cgaatgcttt tgcgacgttg
     3661 agcggcctca cctgggggat ctcaaaaagc gaatgcgtac agaagttttc atataccgat
     3721 gcggatatcc gcgcattctc accgtcggac gacagtcgct acgagaaccg tatcatgaat
     3781 tacatcgttg cgatcgattc agacctcaaa tccgatatca cgatttttcg aactgttaaa
     3841 tcccccatcc gtgatctgct gttcgtgaag aaccgcctct actccatcct tgaggattac
     3901 ggtacggttt ctgccgcggt tgagcgtcgt acgcttg
//
LOCUS       DAORHF010000313         3915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25307_length_3915_cov_4.344560, whole genome shotgun sequence.
ACCESSION   DAORHF010000313 DAORHF010000000
VERSION     DAORHF010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25307_length_3915_cov_4.344560"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..134)
                     /locus_tag="PKJ16_17385"
     CDS             complement(9..134)
                     /locus_tag="PKJ16_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48820.1"
                     /translation="MIAKKKLKLDRPKEGTASVRLYTNDPADPVVELKVRNRVMP"
     gene            complement(204..380)
                     /locus_tag="PKJ16_17390"
     CDS             complement(204..380)
                     /locus_tag="PKJ16_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48821.1"
                     /translation="MIFIILAVCAACTRTPRIHLDAREHHIGDVTTPGQYSHVFHFKN
                     VGTGTLVIEKVKPG"
     gene            complement(454..1512)
                     /locus_tag="PKJ16_17395"
     CDS             complement(454..1512)
                     /locus_tag="PKJ16_17395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV48822.1"
                     /translation="MRKIIRPITIKAIAVLLILSPFLNIYEYLFFGRTSHWLLVRHDF
                     SFQSVIGLVTMLLAIFSGIGLYYIKRWGWYLFLGFSGYVTVFSVIRLFMVKTGFAVMI
                     TSLLVVVLAITIYLISRNTRLPYLSNQFRGWRIRDRIPLAIPVQIQLDENPDVSIATK
                     TIDFSELGALVELAPDAYPLTLGQRMQIQFNIGARDPIILSGVVVQVRESAPGAFLVG
                     LKFVRVRRDKRAIIERFINAHYTPRYRVALTAECRNEKGEKVRAKTWNVSSGGCYISA
                     TGTTFEKDDRVRIELHIPASSGHRTVLCAGTVVWVNHDDRFGKPPGFGITITKFKKGR
                     IAFSRLLLRLSRTAEMAR"
     gene            complement(1679..2443)
                     /locus_tag="PKJ16_17400"
     CDS             complement(1679..2443)
                     /locus_tag="PKJ16_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020443142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV48823.1"
                     /translation="MKNLFDLTGKIALVSGASRGIGESIAKTFAAYGAEVVLTSRKRE
                     GLTVVEKAIVADGGKAKSIPCHNGKMEEIIALFKQIESEYGRIDILVNNAATNFYFGD
                     VLNAPEAAWDKTMEVNLKGYFFMSQHAAKMMVAKGGGSIVNVASINGIRPAPLQGVYS
                     ITKAGVIAMTKSFAKELAPYKIRVNALLPGLTDTKFSSVMIENQELMDTLILPAIPLH
                     RAGQPDEMAGAALYLASDASSFTTGATIVVDGGALA"
     gene            complement(2495..3403)
                     /locus_tag="PKJ16_17405"
     CDS             complement(2495..3403)
                     /locus_tag="PKJ16_17405"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HNV48824.1"
                     /translation="MKIDDVKKVLIIGGGTMGQQISVPCAIGGYDVVIYDIKNEVLQT
                     ARERIGRLVQGFVDWKKITPEEADAALARISMTTDAAAAAADADIVSESVPEDPSLKG
                     KIFSQFNALCPERTVFTTNTSTLLPSMFAEATGRPDRFLAFHFHDIRITNVVDIMPHT
                     GTDPAVVSLVKAFAERTGQLPIMMTKESHGYVFNAMISTLFFSAQTLAANGVASVEDI
                     DRAWMGVTHMMVGPFGMMDSVGIDTVWHITNYWAQKRKDPQAMKNADLMKRYVDEGRL
                     GQKTRKGFYEYPNPKYSQPEFLKGAH"
     gene            complement(3416..>3915)
                     /locus_tag="PKJ16_17410"
     CDS             complement(3416..>3915)
                     /locus_tag="PKJ16_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007152026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HNV48825.1"
                     /translation="FERVMAWLHDTMPPDCPRVSIIHNDFKFDNAVLNPDDPVEITGL
                     LDWEMATIGDPLMDLGSSLAYWIEKDDPPNMQAIRLMPTHLDGMLTRREQVARYSAKT
                     GIAVGSFDYYYCFGLFRLAVIAQQIYYRYVHGQTKDERFKMLIFAVKILEETARAVID
                     ATDHR"
BASE COUNT          753 a         1157 c         1181 g          824 t
ORIGIN      
        1 gaacgggctc agggcatcac ccgattgcgg accttgagct ccacaaccgg gtctgccgga
       61 tcgtttgtgt acagccgtac tgaagcggtc ccctccttgg gacggtcaag cttcagtttt
      121 ttcttcgcga tcatgatcaa ttccagcgtt ccctggccgc cggccgggat ctccgtgctc
      181 gaaagccggt aggtgtcgca ccctcacccg ggttttacct tttcaatgac gagcgtgccg
      241 gtccccacgt tcttgaaatg aaatacgtgc gagtactgtc ccggcgtcgt cacgtcgccg
      301 atatgatgct ccctggcgtc gaggtggatg cgcggggtcc gcgtgcatgc cgcgcacacg
      361 gccagtatga taaaaatcat cgcgagcgct tcgcggatca accgtgcgcg tggtgcgttt
      421 tttcgcagta tgtgttgcat ggccgggaac cgcctatcgc gccatctccg ccgttctcga
      481 gagccgaaga agcagccgcg agaaagcgat gcgccctttc ttgaatttcg ttatggtgat
      541 cccgaaaccg ggcggtttgc cgaagcggtc atcatggttg acccagacga ccgttccggc
      601 gcagagcacg gtccgatgcc cggacgaggc gggaatgtgc agttcgatgc ggacacggtc
      661 gtccttctcg aacgtggtcc cagttgcgga tatataacag ccgcccgaag atacgttcca
      721 cgtcttcgcg cggacctttt cgcccttttc attgcggcac tccgcggtaa gcgcgacacg
      781 atagcgcgga gtataatgcg cgttgataaa ccgctcgatg atggcgcgct tgtcccgccg
      841 cactcgcacg aacttgagac ccaccaaaaa agcgcccggc gcgctttcgc gaacctgcac
      901 aacgacgccc gaaaggatga tcgggtcgcg cgcgccgatg ttgaactgta tttgcattcg
      961 ctgcccgagc gtaagcggat aggcgtcggg agcgagttcc accagcgcgc ccagctcgga
     1021 gaagtctatc gttttggtgg cgatcgagac gtcaggattt tcgtccaact gtatctggac
     1081 cgggatcgca agcgggatgc ggtcccgaat gcgccatccg cggaactggt tggagaggta
     1141 gggcaggcgg gtattccgcg agatgaggta gatggtgatc gcgagcacca ccacgaggag
     1201 cgaggtgatc atgaccgcga aaccggtttt taccatgaag aggcgtatca cgctgaacac
     1261 ggtcacatag cccgagaacc cgagaaagag ataccacccc catcgcttga tataatacag
     1321 gccaatgccg gagaagatcg ccagcagcat ggtcacaagc ccgatgaccg actggaacga
     1381 aaaatcatga cggacgagca gccagtggga ggtgcggccg aaaaacaggt attcgtagat
     1441 attgagaaac ggcgagagga tgagcaggac ggcgatggct ttgatggtga tgggccgtat
     1501 gatttttctc atggatatcg ccaacggaac cgcggataat gtgaacgtac ggcggtgatg
     1561 attgggcggg aaagcaaata tgtcaataca aatacgcgcg gtgcgtcatc aggttgttcg
     1621 ctccccggcc catcggcgag tgattcctct accgccgaca cgccggggcg caattgcgtc
     1681 aggcgagcgc gccgccgtca acgacgatgg tcgcgccggt ggtgaatgac gatgcatcgg
     1741 aggcgaggta gagcgccgcg cccgccatct cgtccggctg ccccgcccga tgcaggggga
     1801 tcgcgggcag gattagcgta tccatgagct cctggttctc gatcatgacc gaggagaact
     1861 tggtgtccgt gagccccggg agaagcgcat tgacgcggat tttgtagggc gcgagctcct
     1921 tcgcgaacga ctttgtcatg gcaatcacgc cggccttggt gatggagtag acgccctgca
     1981 ggggcgccgg ccgaatgccg ttgatggacg cgacattgac gatcgagccg ccgcccttcg
     2041 cgaccatcat tttcgcggca tgctggctca tgaaaaaata ccccttgagg tttacctcca
     2101 tggtcttgtc ccatgccgcc tccggagcgt tcagcacgtc gccgaaatag aaattcgtgg
     2161 cggcgttgtt gaccagtatg tcgatgcgtc catactcgct ctcgatttgc ttgaagagag
     2221 cgatgatctc ttccatttta ccgttgtggc agggaatgga tttcgctttc ccgccatcgg
     2281 cgacaatcgc cttttcgacg accgtcaatc cttcacgctt ccggctggtg agcaccacct
     2341 cggcgccgta cgccgcgaag gtcttcgcga tcgattcccc gatcccgcgg ctcgcaccgg
     2401 acacgagcgc tattttcccg gtaagatcaa acaggttctt catgatgctt cctccgctgt
     2461 tatcgtatcg tagatgtcct tcggcgctct tgtatcaatg cgcccctttg aggaattccg
     2521 gctggctgta tttcggattc gggtactcgt agaatccctt tcgcgtcttc tggcccagcc
     2581 gtccctcgtc gacataccgc ttcatgaggt ccgcgttctt catcgcctgc gggtcctttc
     2641 gtttctgcgc ccagtagttg gtgatatgcc agacggtgtc gatgcccacg ctgtccatca
     2701 tgccgaaggg gcccaccatc atgtgcgtca cgcccatcca ggcgcggtcg atgtcctcca
     2761 cggacgcgac gccgttcgcc gcgagcgtct gcgccgagaa gaacagcgtc gatatcatcg
     2821 cattgaatac gtagccgtgg ctttccttgg tcatcatgat gggaagctgt ccggtccgct
     2881 cagcgaacgc cttcaccagc gacaccacgg cgggatcggt gcccgtgtgc ggcatgatgt
     2941 ccacgacgtt ggtgatgcgg atgtcgtgga agtgaaaggc gagaaagcgg tcgggacgac
     3001 cggtcgcctc cgcgaacatc gacgggagca gggtggaggt gttggtcgtg aacacggtcc
     3061 gctccgggca cagcgcatta aactgcgaaa agatctttcc cttgagcgaa gggtcctcgg
     3121 ggaccgattc gcttacgatg tcggcgtcgg ccgcggccgc cgcggcgtcg gtcgtcatgc
     3181 tgatccgcgc gagcgcggcg tcggcctcct ccggcgtgat cttcttccag tcgacgaagc
     3241 cctgcacgag ccgtccgatc cgctcgcgcg cggtctgcag cacctcgttc ttgatgtcgt
     3301 agatcacgac atcgtagccg ccgatcgcgc agggaacgct gatctgctgt cccatcgtgc
     3361 cgccgccgat tatgagcacc tttttcacat catcaatctt cattctctcc ctcccttatc
     3421 gatggtccgt cgcgtcgatc accgcacgcg ccgtctcctc gaggatcttc accgcgaaga
     3481 tgagcatctt gaaacgctcg tccttggtct ggccgtgaac gtatcgatag tagatctgtt
     3541 gcgcaatcac cgcgaggcga aacaatccga agcaatagta gtagtcgaac gaaccgacgg
     3601 cgatgccggt cttcgccgaa tagcgggcga cctgctcgcg ccgggtgagc atcccgtcga
     3661 gatgcgtggg catgagccgt atggcctgca tgttcggcgg atcgtccttt tcgatccaat
     3721 aggcgagaga cgagcccagg tccatcagcg gatcgccgat ggtcgccatc tcccagtcga
     3781 ggagcccggt gatctcgacc gggtcgtccg gattgagcac cgcattatcg aacttgaagt
     3841 cgttgtggat gatggacacg cgcgggcagt cgggcggcat ggtgtcatgc agccacgcca
     3901 tcacccgctc gaaat
//
LOCUS       DAORHF010000314         3891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25522_length_3891_cov_4.592544, whole genome shotgun sequence.
ACCESSION   DAORHF010000314 DAORHF010000000
VERSION     DAORHF010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25522_length_3891_cov_4.592544"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PKJ16_17415"
     CDS             complement(<1..588)
                     /locus_tag="PKJ16_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48826.1"
                     /translation="MKQTIRRTLPAILFFLMAAPSLLSAQVAVGVIPFKGEGGDPRAL
                     EAKVRELLVKTKGVVVVADSMMKDIVKIHEQAMASGTAYHDISKLRVAEFIITGSLAG
                     NSLTVKAVDVNTTGEVFNMTVDLSSARDYQIRKVSRELQNAIITSAGSKNRNIPADAK
                     PYMALVKDFIAALPQGDQACWKYLAFYHAGSYQRPV"
     gene            870..2057
                     /locus_tag="PKJ16_17420"
     CDS             870..2057
                     /locus_tag="PKJ16_17420"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HNV48827.1"
                     /translation="MLKDNDAVIVSAVRTPLGSYNGSLAGIGATDLGGIVIAEAVKRA
                     GIDMALVQEIFMGIVLPCGYGQNPARQAAIKAGVPMQSAALSINKVCGSALMAVVLAS
                     ELVKSGYADICVAGGMENMSAAPYFMPKARWGVRMGNAPLMDHMVYDGLWDVVNDRHM
                     GGCNDFISREWSVSREDQDRYALSSYERALAAIANGQFSDEIVPVPLKDRKGNTVHFA
                     VDECPRPTDYDALSKMRPAFNKDGFATAGNSSVISDGAAAVVVMSYAKAKELGAKPMV
                     KIVASGTAGAELDFVLIAPIFSIPKVCAKAGIDVARIDLHEINEAFAGSMISVMRKLD
                     LDRERVNVHGGGIALGHPIGASGARVLTTLVHEMKRRGSALGQASLCLGGAEAVTMIV
                     ENI"
     gene            complement(2032..2490)
                     /locus_tag="PKJ16_17425"
     CDS             complement(2032..2490)
                     /locus_tag="PKJ16_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HNV48828.1"
                     /translation="MNRRPSWEDYFMKIADDVATRTTCVRRGVGAVVVKDKRILTTGY
                     NGPPAGISHCTHETCVRERLKVPSGERHELCRGLHAEQNAIIQAALHGVSINGATIYV
                     THQPCSICTKMLINSGIRTIVFREAYADALAAEMIDEAGVELIRYSPRSS"
     gene            2670..>3891
                     /gene="eno"
                     /locus_tag="PKJ16_17430"
     CDS             2670..>3891
                     /gene="eno"
                     /locus_tag="PKJ16_17430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008721464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HNV48829.1"
                     /translation="MTIITDVHAREILDSRGNPTIEVEVQLSSGVVGRAAVPSGASTG
                     EHEAVELRDGDTARYGGKGVLKAVENVNDAIAQAVVEMDALNQVEIDRIMIELDGTPN
                     KAKLGANAILGVSMACARAVAETYGMPLYRYLGGVGACELPVPMANIMNGGAHSDAPI
                     DLQEYMVMPVGATNFHDGIRWVAEIFQALKGILKGRGLNTAVGDEGGFAPNLSDNEEP
                     LKVIVQAIEKAGYQPGAQVMIALDPAASEFYDSSKGKYVFKKSDKRELSPAQMVDFYA
                     DLVSKYPIISIEDGMAEDDWDGWKLMTEKCGDRIQVVGDDLFVTNTQRLAMGIEKGIA
                     NSILIKVNQIGTLTETVEAIEMAKRAGYTAVVSHRSGETEDTFIADLVVGMNTGQIKT
                     GSASRTDRVAKYNQL"
BASE COUNT          722 a         1175 c         1289 g          705 t
ORIGIN      
        1 gacggggcgc tgatatgaac cggcatgata gaacgcgagg tatttccagc aggcctgatc
       61 gccctggggc agcgcggcga tgaagtcctt caccagcgcc atgtagggtt tcgcgtcagc
      121 cgggatgttt cggtttttcg atcccgcaga cgtgataatc gcgttctgga gctcgcgact
      181 gaccttccgg atctgataat cgcgcgcgct cgacaggtcc accgtcatgt tgaagacctc
      241 gcccgtcgtg tttacgtcca ccgccttgac cgtgaggctg ttgcccgcca ggctgccggt
      301 gatgatgaac tccgcgacgc ggagcttcga gatgtcgtga tacgccgtgc ccgaggccat
      361 cgcctgttca tggatcttga cgatgtcctt catcatggag tcggccacca ccacgacgcc
      421 cttggttttc acgaggagct cgcggacctt cgcctccagg gccctcggat cgccgccctc
      481 gcccttgaag gggataacgc ccacggcgac ctgcgcggag agcagggagg gggccgccat
      541 gaggaaaaac agaatggcgg gcagcgttcg tcgtatcgtt tgtttcatgg ggggcctccg
      601 tcggtaaatc cgagctaacg aaatcttctc ccggcagtgt gcgggcggtt gtgaaatgtc
      661 cgcaactctt tttcgagggt tgatggcgaa aacgggcaaa cggacctgtt tcgctgaaat
      721 tgacttgcat ttttccgccg tccagcatgg aatgagagcg tatgcaaccg gcttccgcga
      781 ggggccggtt gtttgttgcc cgctccatcg ccgtggcgtg ccgcgcggga ctatcaccac
      841 attacgagag ataatgaaag gaaaccacaa tgctcaagga caatgacgcc gtaatcgtga
      901 gcgccgtgcg cacgccgctc ggatcgtaca atgggagcct ggcggggatc ggggccaccg
      961 atctcggcgg tatcgtgatc gccgaggccg tgaagcgcgc cgggatcgac atggcgctgg
     1021 tgcaggagat ctttatgggc atcgtgcttc cgtgcgggta cgggcagaac cccgcccggc
     1081 aggccgccat caaggcgggc gttcccatgc aatcggcggc gctcagcata aacaaggtgt
     1141 gcggatcggc gctcatggcg gtcgtgctcg cctcggagtt ggtcaagtcg ggatatgcag
     1201 acatctgcgt cgccggcggg atggagaaca tgagcgccgc gccgtatttc atgcccaagg
     1261 cccgctgggg cgttcgcatg ggtaatgcgc ccctgatgga ccacatggtg tacgatggcc
     1321 tgtgggacgt cgtgaacgat cgccacatgg gcggatgcaa tgacttcatc tcccgcgagt
     1381 ggagcgtgag ccgtgaggac caggaccgct atgccctgag ctcctacgag cgcgccctgg
     1441 ccgcaatcgc gaacggacag ttttccgacg agatcgtgcc ggtgcccctg aaggaccgaa
     1501 agggcaacac cgtccacttt gccgtcgacg agtgcccgcg tccgaccgac tacgatgcgc
     1561 tgtcgaaaat gcgcccggca ttcaataaag acggtttcgc gacggcgggc aactcctcgg
     1621 tgatcagcga cggcgcggcg gcggtcgtgg tgatgagcta cgcaaaggcg aaggagctcg
     1681 gggcgaagcc gatggtgaaa atcgttgctt cgggcacggc cggcgcggag ctcgatttcg
     1741 tgcttatcgc gccgatattt tccatcccga aggtgtgcgc caaggcgggc atcgatgtcg
     1801 cgagaatcga cctccacgag atcaacgagg ccttcgcggg ctcaatgatc tcggtgatgc
     1861 gcaagctgga cctcgaccgc gagcgggtca acgtgcacgg cggcgggatc gcgctggggc
     1921 acccgatcgg cgcttccggc gcgcgggtgc tcacgacgct tgttcacgag atgaagcggc
     1981 gcggttcggc gctcggtcag gcgtcgctgt gcctgggcgg cgcggaggcc gtcacgatga
     2041 tcgtggagaa tatctgatca gctcgacgcc cgcctcgtcg atcatctccg ccgcgagcgc
     2101 atcggcgtag gcctcgcgaa agacgatcgt gcggatgccg ctgttgatga gcatcttggt
     2161 gcagatggag cacggctggt gcgttacgta gatcgtcgcg ccgttgatgg acacgccgtg
     2221 cagcgccgcc tggatgatgg cgttctgctc ggcgtggagc ccccggcaca gctcgtggcg
     2281 ctcgcccgag gggaccttca gccgctcgcg cacgcaggtc tcgtgcgtgc agtgcgagat
     2341 tcccgccggc ggcccgttgt atccggtggt gagaatgcgc ttgtccttca cgaccaccgc
     2401 gcccacgccg cgccgcacgc aggtggtccg cgtcgccacg tcgtcggcga tcttcatgaa
     2461 ataatcttcc cagctcggtc gtctgttcac ggccttcgct ccatttcacg cctgatgatt
     2521 gccgcccgac gcggcggtcc cggcatgctg gcatcagcgc gacgcatttg gcaaggacta
     2581 ttcgcggtgg cgcgcgggaa aaaatatgct tgattctttg aaaaactatg aataggctgc
     2641 acgtaagaaa ctgcgaaggg agcggccaca tgaccatcat caccgacgtc cacgcacgcg
     2701 agatcctcga ttcgcgcggc aatcccacca tcgaggtcga ggtgcagctt tcgtccggcg
     2761 ttgtcggacg ggccgcggtc ccctccggcg cctcgaccgg cgagcatgag gccgttgagc
     2821 tccgcgacgg cgacacggcg cgctacggcg ggaagggggt gctcaaggcg gtcgagaacg
     2881 tgaacgacgc catcgcgcag gccgtcgtcg agatggacgc gctcaaccag gtcgagatcg
     2941 accggatcat gattgagctg gacgggaccc ccaacaaggc gaagctcggt gcgaacgcca
     3001 tcctgggcgt ttccatggcc tgcgcccgcg cggtcgccga gacctacggc atgccgctgt
     3061 accggtatct tggcggcgtg ggcgcctgcg agctcccggt gcccatggcc aatatcatga
     3121 acggcggcgc gcattccgat gcgcccatcg acctacagga atacatggtg atgcccgtcg
     3181 gcgcgaccaa ctttcatgac ggcattcgct gggtggccga gatattccag gcgctcaagg
     3241 gcatcctcaa gggcaggggc ctcaacaccg ccgtgggcga cgagggcggg ttcgcgccga
     3301 atttgtccga caacgaggag ccgctcaagg tgatcgttca ggccatcgag aaggcgggct
     3361 accagcccgg cgcacaggtg atgatcgcgc tcgatccggc ggcgagcgag ttctacgatt
     3421 cgtccaaggg caagtacgta ttcaagaaat ccgacaagcg ggagctgtcc cccgcgcaga
     3481 tggtcgattt ctacgccgat ctcgtctcga agtatccgat catctcgatc gaggacggca
     3541 tggcggagga cgattgggac ggctggaagc tcatgaccga gaagtgcggc gaccgcatcc
     3601 aggtcgtggg cgacgatctc ttcgtgacca atacccagcg gctggcgatg ggcatcgaaa
     3661 agggtattgc gaactccatc ctcatcaagg tgaaccagat cgggacgctc accgagaccg
     3721 tcgaggcgat cgagatggcg aagcgcgccg ggtacaccgc cgtggtgtcg caccgatcgg
     3781 gcgagaccga ggacaccttc atcgccgacc tcgtggtggg gatgaatacc gggcagatca
     3841 agaccggatc ggcgtcgcgc accgaccgcg tggccaagta caaccagctc a
//
LOCUS       DAORHF010000315         3886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25567_length_3886_cov_5.116680, whole genome shotgun sequence.
ACCESSION   DAORHF010000315 DAORHF010000000
VERSION     DAORHF010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3886
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25567_length_3886_cov_5.116680"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="PKJ16_17435"
     CDS             complement(<1..589)
                     /locus_tag="PKJ16_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HNV48830.1"
                     /translation="MLAKRVIPCLDVKDGRVVKGVNFVNLIDAGDPVENGKFYDAEGA
                     DELVFLDITASSDRRDIIIEMVKRVAETVHIPFAVGGGIRTVDDVRRILENGADKVSI
                     NTAAVLDPSILDDSAVRFGSQCIVVAIDAKRNDAGGWTVYLHGGRTPTEHDAIQWARQ
                     ACERGAGEILLTSMDRDGTRIGYDLELTRAIADAVP"
     gene            complement(579..2198)
                     /locus_tag="PKJ16_17440"
     CDS             complement(579..2198)
                     /locus_tag="PKJ16_17440"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HNV48831.1"
                     /translation="MRIVFAGIALVFAALAAYVYDCNGPRSFRFTDPLDYRDVSAHNA
                     RYNASKQHRWPLRYDAVIIAVLDGVRRDYLYNAEIAPNITARWRVGGMRFENAQCMLP
                     SVSAPNYYAIMTGAPPFLNGITNNARRFQRHARIPTVFHHLKQHGRAGEVIGFVWYGD
                     LAAGQAQYIQVERGREDTPGVTAALLSAVQSNSLSPLTLVHYTAPDTRAHQTRSNASP
                     EYLHAVTSIDSDLGRVFRELDARARNACVIVMADHGMDSDGHHGGSDALSLDIPLYIL
                     ARDIPHGVVRRQVHNISIAPTVAAMLGAPLPDCAAGTIIHEVVGADRRVSYLTESIEK
                     KGHLIGMLRGSAATAGFAVGGDIVTSLESRDLDQTRDILGFVEERNAESLFYQRVGAI
                     ACAIALIAWVVFKSGHGVPTLVALNALYCAGIAVGKYMVTSNARFTEAVAAYFVALVL
                     GALLFRAIFMDRALADQLALRAPGAHIVALLLCSSIVAAIAVIPLHPLVPDQHLYAFR
                     FSMALFAHPVLFAGLMFLLAAQRRREAPDAG"
     gene            complement(2198..2584)
                     /locus_tag="PKJ16_17445"
     CDS             complement(2198..2584)
                     /locus_tag="PKJ16_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48832.1"
                     /translation="MRTKFSDRDETMNTRTRHALALILLAGVIAAPAGCSKIDERLIG
                     KWEAEIKAPAGIRTVMMLEFKKNGSMRVRLGKGGPVLGMRYSAHDGTITVTERGASQS
                     IPYSFTDDRLVLSYPLLGELNFKRAH"
     gene            2666..3775
                     /locus_tag="PKJ16_17450"
     CDS             2666..3775
                     /locus_tag="PKJ16_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV48833.1"
                     /translation="MAASKKTTGAAQSAFRGFNARHSPAIDRFIASFFKGKIRSAQFP
                     FIADCYRDLSAYCLRPGKRLRPLVLIAAYLGYGGDPKRIGEITPIAASLELMHSFLLV
                     QDDVIDRSDVRRGGKALHVLMGERYRGAVNNEHVGNDVALILGDVLFANAIEIISSAR
                     IDGDVRTEFMKLFANTYELTAWGQILDIMHSLPADIAPGNGVSTSIGLLKTAYYTVLN
                     PMCMGHVLSGRVRGVAGLVERVPIMQFALPLGMAFQLRDDILGVYGKKKDTGKPSDSD
                     IAEGKLTLFVELAVSSLSDAERKRFISRFSAPRKTAKDIAAIRDVFDRTGVREIATDR
                     LGVLIRQAREALPGCRLREAERKVFDSLVDEVARV"
BASE COUNT          665 a         1220 c         1330 g          671 t
ORIGIN      
        1 ccgggaccgc gtcggcgatg gcgcgcgtga gctcgagatc gtatccgatc ctggtcccgt
       61 cgcggtccat gctggtcagg agtatctcgc ccgccccgcg ctcgcaggcc tggcgcgccc
      121 attggatcgc gtcatgttcg gtgggggtgc gcccgccgtg caggtatacg gtccagccgc
      181 ccgcatcgtt gcgtttcgcg tcgatcgcca ccacgatgca ctgcgagccg aagcgcacgg
      241 ccgaatcgtc gagaatggac ggatcaagaa cggcggcggt gttgatggag accttgtcgg
      301 cgccgttctc caggatgcgg cgcacatcgt cgaccgtgcg gatgcccccg cccaccgcga
      361 agggtatgtg gaccgtttcg gccacgcgtt tgaccatttc gatgatgatg tcgcgccggt
      421 cgcttgacgc ggtgatgtcg aggaagacga gctcgtcggc cccctcggcg tcgtagaact
      481 tcccgttctc gaccgggtcg ccggcgtcga tcaggttcac gaagttcacg cccttgacca
      541 cgcggccgtc cttgacgtcg agacagggga tgacgcgctt agccagcatc gggcgcctcg
      601 cgcctgcgtt gcgcggccag cagaaacatg aggccggcga agaggacggg gtgcgcgaac
      661 aacgccatgg aaaacctgaa ggcgtacagg tgctggtcgg gcacgagcgg atgaagcggg
      721 atgacggcga tggccgcgac gatggacgag cacagaagga gcgcgacgat gtgggcgccc
      781 ggagcgcgca gcgcaagctg atccgcgagc gcacggtcca tgaagatcgc ccgaaacaag
      841 agcgcgccca ggaccagggc gacaaagtag gccgcgaccg cctcggtgaa gcgggcattt
      901 gatgtcacca tgtatttccc gacggcgatg ccggcgcagt agagcgcgtt gagcgcgaca
      961 agcgtcggga cgccgtgccc gctcttgaac acgacccatg cgatgagggc gatcgcgcag
     1021 gcgatggcgc cgacacgctg gtagaagagc gactccgcgt tgcgctcctc gacgaatccg
     1081 aggatgtccc tggtctggtc gaggtcgcgg ctctccaggc tcgtgacgat gtcgccgccg
     1141 acggcgaacc cggcggtggc ggcgcttccg cgcaacatgc cgataaggtg ccccttcttc
     1201 tcgatggact cggtgagata cgatacccgt cggtccgcgc cgacgacctc gtggatgatc
     1261 gtgccggcgg cgcaatcggg caggggggcg cccagcatgg ccgcgacggt gggcgcgatc
     1321 gaaatattgt gaacctgccg ccgcaccacg ccgtggggga tgtcgcgcgc caggatatag
     1381 agcgggatgt cgagcgagag ggcgtcggag ccgccatgat ggccgtctga gtccataccg
     1441 tggtcggcca tgacgatcac gcaggcgttt cgcgcgcgcg cgtcgagctc ccggaagacg
     1501 cgacccaggt cgctgtcgat ggacgtgacg gcgtgaaggt actcggggct cgcgttcgag
     1561 cgtgtctggt gcgcgcgggt gtcgggcgcg gtatagtgga ccagggtgag gggcgagagc
     1621 gaattcgact ggaccgcgct caggagcgcc gcggtgacgc ccggggtgtc ctcgcgcccg
     1681 cgctccacct ggatgtactg cgcctgccct gcggcgagat cgccgtacca gacgaaaccg
     1741 atcacctcgc ccgcgcgccc gtgttgcttc aggtgatgga agaccgtggg aatgcgcgca
     1801 tgccgctgga agcgacgggc gttgttggtg atgccgttta aaaacggcgg cgcgccggtc
     1861 atgatcgcgt agtagttggg cgcgctcacc gagggaagca tgcactgcgc gttctcgaag
     1921 cgcatgccgc ccacccgcca gcgcgcggtg atgttcggcg cgatctcggc attgtatagg
     1981 tagtcgcgcc gcaccccgtc gagcaccgcg atgatcaccg cgtcgtaccg cagcggccag
     2041 cgatgctgtt tgctcgcatt gtatcgcgcg ttgtgcgcac tgacgtcacg gtagtcgagc
     2101 gggtcggtga accggaagga gcgcggccca ttgcagtcgt agacatatgc cgcgagggcg
     2161 gcgaagacga gcgcgatgcc ggcgaagacg atccgcatta gtgagcccgt ttgaagttca
     2221 gctcgcccag tagcgggtag ctcaggacaa gccggtcgtc ggtaaaggag tacgggatgc
     2281 tctgactggc cccccgctcc gtgacggtga tggtcccgtc gtgggcgctg tagcgcatgc
     2341 cgagcacggg cccgcccttg cccagccgga cgcgcatgct cccgttcttc ttaaactcca
     2401 gcatcatgac cgtccggatc ccggcggggg ccttgatctc cgcctcccat ttgccgatga
     2461 gacgctcgtc gatcttcgaa cagcctgccg gcgcggcgat cacgcccgca agaaggatga
     2521 gcgccagtgc gtgtcgtgtg cgtgtgttca tggtctcgtc gcggtcgctg aatttcgtgc
     2581 gcactatatc gtccgattgg cggcggcggc aaggatattt ttcccattga caaaaaatag
     2641 tggcggcact gtattccgtt cacctatggc agcatcgaaa aaaacgacgg gcgccgcaca
     2701 gagcgccttc cgcggcttta acgcgcgcca ttcgccggcg atcgaccggt tcatcgcatc
     2761 attcttcaag gggaagatcc gctccgcgca gttcccgttt atcgccgatt gctaccgcga
     2821 cctctccgcg tattgcctgc gccccgggaa gcggctgcgt ccgctcgtcc tcatcgccgc
     2881 ctatctcggg tacgggggcg atccgaagcg catcggcgag atcacgccca tcgccgcgtc
     2941 gctcgagctc atgcactcgt tcctgctggt gcaggacgac gtgatcgacc gttcggacgt
     3001 gcggcgcggc ggaaaggcgc tccatgtgct gatgggcgag cgctatcggg gcgcggtgaa
     3061 caacgagcac gtcgggaacg acgtcgccct catcctcggc gacgtcttgt tcgcgaacgc
     3121 gatcgagatc atctcctccg cgcgcatcga cggagacgtc cgcaccgagt tcatgaagct
     3181 cttcgcgaac acctacgagc tgacggcgtg ggggcagata ctcgacatca tgcattccct
     3241 tccggccgac atcgcgcccg gcaacggcgt gtcgacgagc atcgggctgc ttaaaacagc
     3301 gtactacacc gtgctcaatc ccatgtgcat gggccatgtc ctctccggtc gcgtacgcgg
     3361 cgttgcggga ctcgtggagc gggtccccat catgcaattt gcgctaccgc tgggcatggc
     3421 cttccagctt cgggacgaca tcctgggcgt gtacggaaaa aaaaaggaca ccggcaagcc
     3481 gtccgattcc gatatcgccg agggcaagct cacgctgttc gtggagctcg cggtttcctc
     3541 gctctctgac gccgagcgaa agcggttcat ctcccgcttc agcgcgccgc gcaaaacggc
     3601 caaggacatc gccgccatcc gcgacgtctt cgatcgcacc ggcgtgcgcg agatcgcgac
     3661 ggaccgcctg ggcgtgctga tccggcaggc gcgcgaggcg ttgcccggct gcaggctgcg
     3721 cgaggcggag cggaaggtct tcgattcgct ggtcgacgag gttgcgcgcg tataaccggg
     3781 ggagtggcca tgaaacagtt gcgaacatac aacggggagc aggccgcgca ggcgcggatc
     3841 ggacaggtgg tgtcggacct gtgcgacgag cggtcgtacc gctcgg
//
LOCUS       DAORHF010000316         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25627_length_3880_cov_5.301438, whole genome shotgun sequence.
ACCESSION   DAORHF010000316 DAORHF010000000
VERSION     DAORHF010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25627_length_3880_cov_5.301438"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="PKJ16_17455"
     CDS             <1..227
                     /locus_tag="PKJ16_17455"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HNV48834.1"
                     /translation="NVQVNAVAPGMVRTDFSKPFWSSPELYDHIVKAVPMGRIAEVRD
                     VVHPVLFLCSEGSDFITGQTIMVDGGATAV"
     gene            434..613
                     /locus_tag="PKJ16_17460"
     CDS             434..613
                     /locus_tag="PKJ16_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48835.1"
                     /translation="MEIQSISGIDIAQRGNIGETRVAHEAERVERAERTQESPRMPER
                     APETTGHGSAVDAYA"
     gene            635..1447
                     /locus_tag="PKJ16_17465"
     CDS             635..1447
                     /locus_tag="PKJ16_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48836.1"
                     /translation="MAITKAEKAAYNDYVKDFKVEIEDNLKSIKDLEARRKKMANISG
                     YLSVELCLKYLKNVQIYMKMSDASQEMLHIRNEKFLDNSRKDFYKVVQLLEEMLGNDI
                     ERSLNDNKEYLRKIDKLNPAQVLKIIRTIMSVFNSLLKRMGESKWKWSFVDLQGRIAV
                     LVKNFINFSDLQKNRDPRMEFYRDRQELMRVCKSVLNEAAKQFRNKYELSTKVPEDIL
                     KSINLLAALRSIHILFGESEDATKLKNTIDALRARLEADEVKDEEDKKKKKK"
     gene            1571..2626
                     /gene="hrcA"
                     /locus_tag="PKJ16_17470"
     CDS             1571..2626
                     /gene="hrcA"
                     /locus_tag="PKJ16_17470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HNV48837.1"
                     /translation="MVKSNKTERVLDERESAVLKALVYEFIVTGKPVGSRSFVQKYSF
                     SISPATMRNIMFDLENLGFLMQPHTSSGRIPTDVGYRFYVDSLLDTYEFVMSEKVRVR
                     EELIKKELQLDKMFASVSKMLSLVSKYASVVLSPKPDFTVVKHVELIPLDSDEILFIL
                     VTRNGMILNKKVSVSTRITQDDIYELSRYLTGELCGYSLYEIKKDIFDRMRVQTNHDY
                     KKGLALDIAQLALTEETDSEIFIDGIENLLRIPEMIEADKLKSLLHLIEEKRLLKSII
                     ERNIDKDGVNTLIGEEVSDLEISGCSIITSSYQIGKSKVGVVGIIGPTRMDYEKVVPL
                     VDYTGKVVTDLLTKMSK"
     gene            2660..3370
                     /locus_tag="PKJ16_17475"
     CDS             2660..3370
                     /locus_tag="PKJ16_17475"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HNV48838.1"
                     /translation="MVENKYVDDSMNKESGNDTMGNEREIPQDEMFEGADPAVETEAA
                     GAPDDGAVREGDGRDALAAELKKRDEEIASLKDVMQRRQADFENFKKRNLKQQEEQRK
                     MAVRDIALDIIAINDDLLRAIEAAEAIPQGESLEASHAAFVEGVHMISRRIEDALTSH
                     GVVAIDTLNAEFDPCVSEAVEIVASDEVETDTVTKVYQKGFMLDDMVIRHAKVRVSRS
                     AKQGAKDVATECATDGSG"
     gene            3448..>3880
                     /locus_tag="PKJ16_17480"
     CDS             3448..>3880
                     /locus_tag="PKJ16_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HNV48839.1"
                     /translation="MSKIIGIDLGTTNSCVAVMVGGEPVVIQNSEGQRTTPSIVAYTD
                     KGERLVGQVAKNQIITNPENTIRSIKRFMGRAFSEVGEEIKTIPYNVMDDGKGGVKVK
                     TQAGEFTPQEISARVLQKMKQTAEDYLGEKVTRAVITVEPAA"
BASE COUNT          922 a         1046 c         1248 g          664 t
ORIGIN      
        1 tcaacgtgca ggtgaacgcg gtggcgcccg gcatggtgcg caccgatttc agcaagccgt
       61 tctggtcgag tccggagctg tacgatcaca tcgtgaaggc ggtcccgatg gggcgcatcg
      121 cggaggtgcg cgatgtggtg catccggtgc tgttcctgtg ctcggagggc tccgacttca
      181 tcaccgggca gacgatcatg gtggatggcg gcgccacggc ggtgtgatgg atggtgcggg
      241 catgcgcggg agcgcatgag attttacttg aaagcatacg ttggtttggg tatagtagcg
      301 acagagcagt cctgagcggg gcgtgtcaca gcatccggat ggggcggggc gtccgcacat
      361 tcgaagcgca aacgccgtcg cggcataatc aggcgatgcg gcccgcgggt ggggccgccg
      421 tggagtaggc gaaatggaaa tacaaagcat cagcggaatc gacatcgcgc agcgcgggaa
      481 catcggcgaa acgcgcgtgg cgcacgaggc cgagcgcgtc gagcgcgcgg agcgaacgca
      541 ggagtccccc cgcatgccgg agcgggcgcc ggagacgacc ggccacggaa gcgccgtgga
      601 cgcgtacgct tagacggcgg ccggatcatc ccccatggca atcaccaagg ccgaaaaggc
      661 ggcgtacaac gactatgtga aggacttcaa ggtcgagatc gaggataacc tgaagtccat
      721 caaggatctc gaggcgcgcc ggaagaagat ggccaacatc tcgggttacc tgagcgtcga
      781 gctgtgcctc aaatacttga aaaacgtcca aatatacatg aagatgagcg acgcctccca
      841 ggagatgctg cacatccgca acgagaagtt tctggataac tcgcgcaagg acttctacaa
      901 ggtcgtccag ctcctcgagg agatgctggg aaacgatatc gagcgctcgc tgaacgacaa
      961 caaggaatac ctgcgcaaga tcgacaagct gaatcccgcc caggtgctca agatcatccg
     1021 caccatcatg agcgtgttca attcccttct gaagcggatg ggggagtcca agtggaaatg
     1081 gtccttcgtg gacctccagg ggcgcatcgc ggtgctggtg aagaacttca tcaacttttc
     1141 cgatctgcag aagaaccgcg acccgcggat ggagttttat cgcgaccggc aggagctgat
     1201 gcgcgtgtgc aaatccgtcc tgaacgaggc ggcgaagcaa ttcaggaaca agtacgagct
     1261 ctccaccaag gtgcccgaag atattctcaa gtcgatcaat ctcctggccg cccttcggtc
     1321 gatccacatc ctttttggcg agtccgagga tgccaccaaa ctcaagaata cgatcgacgc
     1381 cctccgcgcg cgcctcgagg ccgacgaggt caaggacgaa gaagataaaa agaaaaagaa
     1441 aaaataaaaa aagatacatg cccgaccaaa agatgtacat gatttaaaat atgacttgac
     1501 gtggggattt ttttttatta tattcctggc actcagtcga ttagagtgct aataagcatg
     1561 atagcaggct atggtaaaga gtaataagac agaaagggtt ctcgatgagc gcgaaagcgc
     1621 cgttttgaag gcgctcgtgt acgaatttat cgtaaccggt aagccggttg gttcgcgttc
     1681 ctttgtgcaa aagtactctt tttccatatc tccggccacg atgcgtaata tcatgttcga
     1741 cctggagaac ctgggatttc tcatgcagcc gcacacgtcg tccgggcgga tccccacgga
     1801 cgtggggtac cggttttacg tcgattcgct gctcgacacc tacgagttcg tgatgagcga
     1861 gaaggtgcgg gtgcgcgagg agctcatcaa gaaagagctc cagctcgaca agatgttcgc
     1921 ttcagtgagc aagatgctgt ccctggtttc aaaatacgca tcggtggtgc tctcgccaaa
     1981 gcccgatttc accgtggtca agcacgtcga gctcattccg ctcgacagcg acgagatcct
     2041 gtttatcctc gtcacccgaa acggcatgat cctgaacaag aaggtcagcg tttcgacccg
     2101 catcacccag gacgacatct acgagctgtc gcgctatctc accggggagc tctgcgggta
     2161 ctcgctgtat gagatcaaga aggacatctt cgaccggatg cgcgtccaga cgaaccatga
     2221 ttacaagaag gggctggcgc tcgacatcgc gcagttggcg ctcaccgagg agaccgattc
     2281 cgagatcttc atcgacggga tcgagaatct gctgcgcatc ccggagatga tcgaggccga
     2341 caagctcaag agcctgctgc atctcatcga ggagaagcgt ttgctcaaga gcatcatcga
     2401 gcgcaatatc gacaaggacg gcgtgaacac gctcatcggc gaggaggtga gcgacctgga
     2461 gatctcgggc tgcagcatca tcacctcctc gtatcagatc ggaaagagca aggtcggcgt
     2521 ggtgggcatc atcggcccca cgcggatgga ctatgagaag gtggtgcccc tcgtcgatta
     2581 tacgggcaag gtggtcaccg acctgctgac gaaaatgtcc aagtgattgg cgcggcggtc
     2641 cgccgcgcgg gggtatggca tggttgagaa caagtacgtg gatgacagca tgaacaagga
     2701 gagcggtaac gatactatgg gcaatgaacg ggaaatcccg caggacgaga tgttcgaggg
     2761 tgccgatccg gccgtcgaaa ccgaggcggc cggcgctccc gacgacggcg ccgtgcgcga
     2821 gggagacggt cgcgatgcgc tcgcggcgga gctcaagaag cgcgacgagg agatcgcctc
     2881 gctcaaggac gtgatgcagc ggcggcaggc cgacttcgag aatttcaaga agcgcaacct
     2941 gaagcagcag gaggagcagc ggaagatggc ggtgcgcgac atcgcgctgg acatcatcgc
     3001 catcaacgac gatctcctgc gcgccatcga ggcggccgag gccattccgc agggcgagag
     3061 cctcgaggcg tcgcacgcgg cgttcgtgga gggcgtgcac atgatctcgc ggcgcatcga
     3121 ggacgcgctc acgtcgcacg gggtcgtggc gatcgacacg ctcaacgccg agttcgatcc
     3181 gtgcgtgagc gaggccgtcg agatcgtcgc cagcgacgag gtggagaccg acaccgtgac
     3241 gaaggtgtac cagaagggct tcatgctcga cgacatggtg atccgccacg cgaaggtgcg
     3301 cgtgtcgcgg tcggcgaagc agggggcgaa ggacgtcgcg acggagtgcg cgacggacgg
     3361 gtccggatga cgcgcgcggc ggaaacgacg gaagacgcga gacgtagaat ggaagatcga
     3421 caaatcacaa tgcaatgagg taaccatatg agcaaaataa ttggaattga cttgggaacg
     3481 acgaactcct gcgtggccgt gatggtcggc ggcgagccgg tcgtgataca gaactccgag
     3541 gggcagcgga ccacgccgtc catcgtggcc tataccgaca agggcgagcg cttggtcggg
     3601 caggtggcca agaaccagat catcaccaac ccggagaaca ccatccgctc catcaagcgc
     3661 ttcatgggcc gggcgttctc cgaggtgggc gaggagatca aaacgatacc gtacaacgtc
     3721 atggacgacg gcaagggcgg cgtgaaggtg aagacccagg cgggcgagtt cactccgcag
     3781 gagatctccg cgcgcgtgct ccagaagatg aagcagaccg ccgaggacta tctcggcgag
     3841 aaggtgaccc gcgcggtgat caccgtcgag ccggccgcca
//
LOCUS       DAORHF010000317         3873 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25702_length_3873_cov_3.711367, whole genome shotgun sequence.
ACCESSION   DAORHF010000317 DAORHF010000000
VERSION     DAORHF010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25702_length_3873_cov_3.711367"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="PKJ16_17485"
     CDS             <1..349
                     /locus_tag="PKJ16_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48840.1"
                     /translation="TRWNAASFFVKQKYYQDITREIGGTCTRIIAFMETHEREMSRLD
                     EMSALLRAIRDKLASSCDGCAPTGAGEMAGAAALASERLEASFGDGARGMVFSALLRE
                     YAAQQNEGAVLLF"
     gene            370..615
                     /locus_tag="PKJ16_17490"
     CDS             370..615
                     /locus_tag="PKJ16_17490"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48841.1"
                     /translation="MTVREDVGVKNIKAFHADLAALLAEGGDAVLDFSQVRRIDLAVA
                     QVLTAAQRLVRAEGRAIRLKGLNQTVRAQLALAGLAR"
     gene            625..996
                     /locus_tag="PKJ16_17495"
     CDS             625..996
                     /locus_tag="PKJ16_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008871092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48842.1"
                     /translation="MKYIMVIDDSPTIRTSIQFAIKETGYPVVPADNGRVALEKVKEL
                     KEKGDELALVMVDVNMPEMDGITFIKHFRQEDRFTPILVLTTESQNEKIKEGKDAGAS
                     GWMVKPFQQPQLLQTVQRLVR"
     gene            1009..1503
                     /locus_tag="PKJ16_17500"
     CDS             1009..1503
                     /locus_tag="PKJ16_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV48843.1"
                     /translation="MYEPSAKSTVLENETLESAQYVTFVIGAEVYGVPVLKVQEIIGM
                     TQITHVPNSLPYMKGVINLRGTVVPVVDMRIKFRMEERAYEVNTVILIVEVRGINIGM
                     IVDAVSDVVEIPIKGIQDTPHFSAKIETDFISGIGNRDDQLVIILDVDRILSQDEVES
                     IERK"
     gene            complement(1684..2265)
                     /locus_tag="PKJ16_17505"
     CDS             complement(1684..2265)
                     /locus_tag="PKJ16_17505"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV48844.1"
                     /translation="MYERYSKKYEKKITIIHPGEFYVSRNDELIGTLLGSCVAVCLHD
                     ASSGLAGMNHFMLPGRISERDIFQDSSAKYGITAINELISHMERAGAPIRNLSAKVFG
                     GGHVLSLEANSVTIPHDNVRLARVLLEHADIPIVASDVGENYTRKILMDVKSGRIYLK
                     KSTRQDVIDFVDGREQTYAQRLGRPAGKAVAGR"
     gene            complement(2282..2872)
                     /locus_tag="PKJ16_17510"
     CDS             complement(2282..2872)
                     /locus_tag="PKJ16_17510"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HNV48845.1"
                     /translation="MIKKTDSQFNKPLYILYPGEFYATKEDCVISTVLGSCMAVCLFD
                     VAKRIGGMGIFIVPGTIGTSGIISDAIASKGIGDIELLLGEIVKIGGDRRYLKAKIFG
                     AGYPADSSSQMEQVMNSNIKFIYEYFTLENMTVERSDLGGEFRRRLYFFPWTGKVYRN
                     ILQNNEESSEFIKLEQEYIDSEFRQKEKFGRVILFD"
     gene            complement(2941..3312)
                     /locus_tag="PKJ16_17515"
     CDS             complement(2941..3312)
                     /locus_tag="PKJ16_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48846.1"
                     /translation="MKHILIIDDSPTIRISVEHALKSLGHAMVCAENGRDALGKIDAL
                     IAQGHDIALCVLDVNMPEMDGISFLKEFRSRDKFTPVLILTTESEDDKIKEGRSHGAS
                     GWIIKPFKPDELLSVAKRFLK"
     gene            complement(3445..>3873)
                     /locus_tag="PKJ16_17520"
     CDS             complement(3445..>3873)
                     /locus_tag="PKJ16_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006921885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HNV48847.1"
                     /translation="VNGQTRLSVKEAVHGDRLYRGIALIAPGNFHMLLRRDATGYWVE
                     INDGPPVNRHRPSVDVLFRSTAQVAGGSAVGVLLTGMGADGAEGLLEMKEAGARTIAQ
                     DEASSTVFGMPCEAIIRGAADMVKNPRGIIAYLGGLTGED"
BASE COUNT          711 a         1147 c         1244 g          771 t
ORIGIN      
        1 cacccgctgg aacgcggcgt cgttcttcgt gaagcagaag tactaccagg acattacccg
       61 ggagatcggc ggcacctgca cgcgcatcat cgccttcatg gagacgcacg agcgcgagat
      121 gtcccggctc gacgagatga gtgcgctgtt gcgcgcgatc cgcgacaagc tcgcgtcctc
      181 gtgcgacggg tgcgcgccga cgggcgccgg cgagatggcc ggggcggcgg cgctggcgtc
      241 ggagcggctc gaggcgagct tcggtgacgg cgcgcggggc atggtgttct ccgcgctgtt
      301 gcgggaatac gcggcgcagc agaacgaggg cgccgtactg ctgttttaag gagggacgcg
      361 atgcggcaga tgaccgttcg ggaagacgtc ggcgtgaaga acatcaaggc ctttcatgcc
      421 gacctggcgg cgctgttggc cgagggcggc gatgcggtgc tcgatttctc gcaggtgcgc
      481 cggatcgacc tggccgtggc gcaggtgctc acggccgcgc agcggctggt gcgcgccgag
      541 gggcgggcga tcaggctcaa gggcctcaat cagacggttc gcgcacagct ggccctcgcg
      601 gggctggcgc gttaggaggg acgcatgaag tacatcatgg tgatcgacga ttcgcccacg
      661 atccgcacga gcatccagtt cgccatcaag gagaccgggt accccgtggt gccggccgac
      721 aacggccgcg tggcgctgga gaaggtgaag gagctcaagg agaagggcga cgagttggcg
      781 ctggtcatgg tggacgtgaa catgcccgag atggacggca tcaccttcat caagcatttt
      841 cgccaggagg accgctttac gcccatcctg gtgctcacga ccgagtcgca aaacgagaag
      901 atcaaggaag gcaaggacgc gggcgcttcg ggctggatgg tgaagccgtt ccagcagccg
      961 cagctcctgc agaccgtcca aagactggtg agataggggg agaggattat gtatgaaccg
     1021 tcggcaaaat cgaccgtgct cgaaaacgag acgctggaat cggctcagta tgtgaccttc
     1081 gtgattggcg cggaggtgta cggcgtgccc gtgctcaagg tgcaggagat catcggcatg
     1141 acgcagatca cgcacgtgcc caacagcctg ccgtacatga agggcgtcat caacctgcgc
     1201 gggaccgtgg tcccggtggt ggacatgcgc atcaagttcc gcatggagga gcgcgcctac
     1261 gaggtgaaca cggtgatcct catcgtggag gtgcgcggca tcaatatcgg catgatcgtg
     1321 gacgcggtct ccgacgtggt ggagatcccc atcaagggca ttcaggacac gccgcacttc
     1381 agcgcgaaga tcgagaccga ctttatcagc gggatcggga accgggacga ccagctggtc
     1441 atcatcctgg acgtggaccg gatcctctcg caggacgagg tcgagtccat cgagcgcaaa
     1501 taggccgatg cggcatcgga atgcatcgaa cgtgctgggt ggcggtggga ggtcttatgt
     1561 ttctgtgact cattcccctt tttaatccca ccaaacaatc atctctgtaa cggcgccggt
     1621 taatccagcg ccgtcttttt ttctcccgtg gcgcggcgcg gcgcggatga taggcgacgg
     1681 cccctagcgt ccggcgacgg cttttccggc ggggcgcccg aggcgctgcg cataggtctg
     1741 ttcgcgccca tcgacaaaat cgataacgtc ctggcgcgtg gatttcttca ggtagatccg
     1801 gccgcttttc acgtccatga ggatcttgcg agtgtagttc tcgcccacgt ccgatgccac
     1861 gatggggatg tcggcatgtt ccagcagcac gcgcgcgagg cgtacattgt cgtgcggtat
     1921 cgtgaccgag ttggcctcaa ggctcaagac gtgccctccg ccgaacacct tcgccgagag
     1981 attcctgatg ggggccccgg cgcgttccat gtgggaaatc agctcgttga tggccgtaat
     2041 gccgtacttc gccgagctgt cctggaagat gtctcgttcc gagatgcgcc ccggcagcat
     2101 gaaatggttc atgccggcca gccccgacga ggcatcgtgc aggcacaccg ccacgcagga
     2161 ccccagcagc gtgccgatga gttcgtcgtt gcgcgaaaca tagaactcgc ccggatggat
     2221 gatggtgatc tttttctcgt attttttgct atagcgctcg tacatggtgc aatgcgttcg
     2281 gtcagtcgaa gaggatcacg cggccgaact tttccttctg gcggaattcc gagtctatgt
     2341 actcttgctc aagcttgatg aactcggacg actcctcgtt gttttgcagg atgttccggt
     2401 acacctttcc cgtccacggg aaaaagtaga ggcgtcggcg gaactctccg ccgaggtcgc
     2461 tgcgctcgac ggtcatgttt tccagggtga agtactcgta gatgaacttg atgttgctgt
     2521 tcatcacctg ctccatctgg gaggagctgt ccgcggggta gccggcccca aagatcttgg
     2581 ccttcaggta ccggcggtcg ccgccgattt tcacgatctc gcccagcaga agctcgatgt
     2641 ccccgatccc cttgctcgcg atggcgtccg atatgatgcc gctggtgccg atggtccccg
     2701 ggacgatgaa aatgcccatt ccgccgatcc tcttggccac gtcaaagagg cacacggcca
     2761 tgcaggaccc gaggaccgtg ctgataacgc agtcctcttt cgtcgcgtag aattcacccg
     2821 ggtagaggat gtagagcggt ttattgaact gggaatcggt tttctttatc atgtcgcctg
     2881 ttcctggtta ttgtccgaga cgatcgttgt gcgcccgacg gatggcatgc gaatgcgcga
     2941 tcacttcaag aaccgcttcg ccacggaaag gagctcgtcg ggcttgaacg gcttgatgat
     3001 ccatcccgac gcgccgtggg aacggccctc ttttatcttg tcatcctcgg attccgttgt
     3061 caggatgagc acgggggtga acttgtcccg tgaccggaat tccttgagaa acgagatgcc
     3121 gtccatctca ggcatgttca cgtcgagcac gcatagcgcg atatcgtggc cctgcgcgat
     3181 cagcgcgtcg atcttgccga gggcgtcccg gccgttttcg gcgcatacca tcgcatggcc
     3241 gagcgacttc aaggcgtgct cgacgctgat ccggatcgtc ggggaatcgt cgatgatgag
     3301 tatatgcttc atgaaacctc ctctgagcgg gcctggcgcg tctcccggtc atgcgccatt
     3361 actcgatact gactgtcaat ccgaaacgac ccgttccgta tgatgtaatc gccgatgcga
     3421 tgtatcgcac ctgcggccca aacgtcaatc ctcgccggta agcccaccga gataggcgat
     3481 gatcccgcgc gggtttttca ccatgtcggc cgcgccgcgg atgatcgcct cgcacggcat
     3541 gccgaagacg gtcgaggacg cctcgtcctg cgcgatggtg cgcgcccccg cctccttcat
     3601 ctcgagcagg ccttccgcgc cgtcggcgcc catgccggtg aggagcaccc cgacggcgct
     3661 tccgccggcg acctgcgccg ttgaccggaa cagcacatcc accgacgggc ggtgacggtt
     3721 caccgggggc ccgtcgttta tctccaccca gtatccggtc gcgtcgcgcc tgagcagcat
     3781 gtggaaattc cccggcgcga tgagggcgat gccccggtag agccggtcgc catggacggc
     3841 ctctttgacg gagagccgtg tctgtccgtt tac
//
LOCUS       DAORHF010000318         3863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25803_length_3863_cov_4.638918, whole genome shotgun sequence.
ACCESSION   DAORHF010000318 DAORHF010000000
VERSION     DAORHF010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3863
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25803_length_3863_cov_4.638918"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PKJ16_17525"
     CDS             <1..569
                     /locus_tag="PKJ16_17525"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HNV48848.1"
                     /translation="ELGTSTLVMGVAVVFIATVSAGLPGATPLTVCGGLLFGAIGALY
                     SVAAATCGAIIAFTVSRRLAGRWLQERFAERLANFNRDFDENGAWYVLAIRMTPLFPS
                     FLVNLLAGLSTLPARSFLLITALGIIPNSLAYTYAGRRLALAETPRDLLSPETMLALA
                     LIALAALVPPAVRKLAHRRGTRTSSQPD"
     gene            642..1193
                     /locus_tag="PKJ16_17530"
     CDS             642..1193
                     /locus_tag="PKJ16_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48849.1"
                     /translation="MAQATALIKAYMEGTIPDNGGFVLSAVFDPGSIYSIYECTAYRN
                     VKDVTRTPDGIVFKSDGNRTHMLVEPPIYPLKHIEPVNRDDGHAIPYRFNELTILTGK
                     KSEKIMVPEEPRMLYTSFTIADRRSDYFAYVFYPTPDVYVAIKKFLADSLYNDCNISK
                     QDSIEAATAMLATIKKFVIWKAD"
     gene            1237..1551
                     /locus_tag="PKJ16_17535"
     CDS             1237..1551
                     /locus_tag="PKJ16_17535"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV48850.1"
                     /translation="MSNKRKHDRANVTIRSEVRSPESITLSKTVNVSRGGLFISTPEP
                     LGEGSELNLTIQVPGGEVLEIRGKVKWVRQNEVDGERAGMGIEFVDASDKDVIELKKI
                     MD"
     gene            1860..2174
                     /locus_tag="PKJ16_17540"
     CDS             1860..2174
                     /locus_tag="PKJ16_17540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48851.1"
                     /translation="MKNHMNEWTFPERIDYNEVPEYLRRFEAEAITGTLVFDLRDTEV
                     AHSSFIGFLIVAKYAIEKSGHDLVLRLSPALERLLAMLHILEYFSVRAHRAHRAEQRL
                     TA"
     gene            2256..2561
                     /locus_tag="PKJ16_17545"
     CDS             2256..2561
                     /locus_tag="PKJ16_17545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HNV48852.1"
                     /translation="MKPMPMLFNDEQVESTCEILKSIAHPIRLKILCFIMEGEKNVTE
                     IEGEFHSTISNISQHLTILRKANILARRKEANYMFYSIKDPCIQVLMKTLKDLYCQA"
     gene            2594..3601
                     /locus_tag="PKJ16_17550"
     CDS             2594..3601
                     /locus_tag="PKJ16_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48853.1"
                     /translation="MNAKSHKTVVAVVVTALVALGSIFACQQENLWDLATLEESHGMF
                     IAAGDNGVILSSSDGKNWTKSGFADPDNTYYSIATDKKGTWMIVGYNNTYGMGIIFSS
                     TDSGKSWKNTTSTNTNGNVLRAISTNQSGAWVVASDSGYYAVSSNSGASWSDSSFSNS
                     IYGIAHNSNRFVTVGSGFGSFHYSTDAGTSWQTAAATTPSFSYCAVTINNAFLSIGTD
                     VWASNDGYTWDYVSSPFGFTREGAASNTDGRVIAVGETPEGSFYSDDSGVSWTLVDPS
                     ATSNLIGITFGKGIWVTVGADSTTMIDGVMHYSTDNGSSWSFNVAPASPVLRCVAYGY
                     R"
     gene            3660..>3863
                     /locus_tag="PKJ16_17555"
     CDS             3660..>3863
                     /locus_tag="PKJ16_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48854.1"
                     /translation="MTAKTLIILGVLCAPACQQENLWELASKDSRREGIFVAVGESGV
                     VITSRDGLQWTPQPSIVNTHLIGI"
BASE COUNT          862 a         1172 c         1073 g          756 t
ORIGIN      
        1 tcgaactcgg cacctcgacg ctggtgatgg gcgtcgcggt cgtcttcatc gcgacggtgt
       61 cggccgggct tccgggggcc acgcccctga ccgtgtgcgg agggcttctc ttcggggcga
      121 tcggcgctct gtactccgtc gccgccgcca cctgcggcgc catcatcgcg tttaccgtgt
      181 cgcgccgcct ggcgggccgg tggctccagg aacgcttcgc cgagcggctg gcgaacttca
      241 accgcgattt cgacgagaac ggggcgtggt acgtgctcgc catccgcatg accccgctct
      301 ttccgtcgtt cctggtgaat ctgcttgccg gcctgtcgac ccttcccgcg cggagcttcc
      361 tgctcatcac cgcgctgggc atcatcccga actcgctcgc ctacacctat gcggggcgcc
      421 ggctcgcgct cgcggaaacg ccgcgcgacc tcctctcgcc cgaaacgatg ctggcgctgg
      481 cgctcatcgc gcttgcggcg cttgtcccgc ccgcggttcg gaaactcgcc caccgccgcg
      541 ggacgcggac atcatcccaa ccggattgaa aaagtgttgc ttttttccct ttgtgccgca
      601 tcctttgcgg gagaacgttt gcgacgacac cgaggaggct tatggcacag gcaaccgcgc
      661 ttatcaaggc ctacatggag gggaccatcc cggacaacgg cggtttcgtc ctgagcgccg
      721 tgttcgaccc gggctcgatc tactccatct acgaatgcac cgcctaccgc aacgtgaagg
      781 acgtgacgcg cacgcccgac ggcatcgtgt tcaagtccga cggcaatcgc actcatatgc
      841 tggtcgagcc gcccatctac ccgctcaagc acatcgaacc ggtaaaccgc gacgacgggc
      901 acgccatccc ataccgtttc aacgagctca ccatcctcac cggaaagaaa agcgagaaga
      961 tcatggtgcc cgaggagccg cgcatgctgt acacctcctt caccatcgcc gaccgccgct
     1021 cggattactt cgcctacgtg ttctacccga cgcccgacgt gtatgtcgcc atcaagaaat
     1081 tccttgcgga cagcctgtac aacgactgca acatctccaa gcaggactcg atcgaggcgg
     1141 cgacggccat gctcgccacc atcaaaaagt tcgtcatctg gaaggccgat taggcgcgac
     1201 ggccggacgc gcacaccgac acacacggag gacgccatga gcaacaagag aaagcacgat
     1261 cgcgcgaacg tcaccatccg gtcggaggtg cgctcgcccg agagcatcac cctgagcaag
     1321 accgtaaacg tgagccgcgg cggattgttc atctccaccc ccgagcccct gggcgagggg
     1381 tccgagctga atctcacgat acaggtcccc ggcggcgagg tgctcgagat ccgcggcaag
     1441 gtaaaatggg tgcgccagaa cgaggtcgat ggcgagcgcg ccgggatggg gatcgagttc
     1501 gttgacgcga gcgacaagga cgtcatcgaa ctcaagaaga tcatggacta acgccgctcg
     1561 cttcgccgca gtccgccccc accgtgggac gaaaacccat cgacggatgg gttttttcgc
     1621 cggccgcttc cagattatgt cgcattattg gcccgccccg acaacagaca ccgcgccatg
     1681 ccgacaaccc tgggttttgc cgggaccatg cgggcgcacc gcggcccatc gccatcctcc
     1741 agccgacggc gcgcaggggg cgggaaaaaa cacgaaaaaa atgtgctaaa cctttatgaa
     1801 aaaaagcacg acaatagtat cataagggat tgaacgaaat caggtcaagg atgaccgcca
     1861 tgaaaaacca catgaacgaa tggaccttcc ccgagcggat tgactataac gaagttcccg
     1921 agtacctgcg ccgattcgag gcggaggcga taaccggcac gctcgtcttc gaccttcggg
     1981 acacggaggt ggcgcactca tcgttcatcg gcttcctcat cgtcgccaag tacgccatcg
     2041 agaagagcgg acacgacctc gtgttgcggc tgtcacccgc gctggagcgg ctacttgcga
     2101 tgctgcatat tctcgagtac ttcagcgtcc gcgcccaccg cgcccaccgc gccgaacagc
     2161 gcctgaccgc ctgatccgcg cgggcatgtt tcgaaaaaaa atttgacaat tcatctcgcg
     2221 cgcgtatatg gtagtatcga tacgcgcgaa tcgccatgaa accgatgccc atgctcttca
     2281 acgacgaaca agtcgaatcc acgtgcgaga tcctcaagtc gatcgcgcac cccattcgac
     2341 tcaagatcct ctgcttcatc atggagggcg agaagaacgt gaccgagatc gagggcgagt
     2401 tccactccac catatccaac atatcgcagc acctcaccat ccttcgcaag gcgaacatcc
     2461 tcgcccgtcg caaggaggcg aattacatgt tctactccat caaggacccc tgcatacagg
     2521 tgctcatgaa aacgctcaag gacctgtact gccaggcgtg aggggcgccg tcgccccaaa
     2581 cggaggaccg gccatgaatg cgaaatcaca caaaaccgtc gtcgccgtcg ttgtcacggc
     2641 gctcgtggcg cttggttcta tcttcgcctg ccagcaggag aacctgtggg atttggcgac
     2701 gctggaagag tcgcacggga tgtttattgc agcgggggat aatggggtga tcctgtcttc
     2761 gagcgatggg aaaaactgga cgaagagtgg ttttgcagac ccagacaata cgtattatag
     2821 tatcgcgaca gacaaaaagg gcacttggat gatcgttggc tacaacaata cttatggtat
     2881 gggtatcatt ttttcttcga cagatagcgg aaaatcctgg aaaaacacga caagcaccaa
     2941 tacaaacggg aacgtgctca gagccatctc gacaaaccaa tccggcgcct gggttgttgc
     3001 cagtgactcc gggtattatg ccgtttcttc aaatagtggc gcttcatgga gtgatagttc
     3061 attctccaac tccatctatg ggattgcgca caattcaaac agattcgtca ccgtgggaag
     3121 cggattcgga agttttcact acagcaccga tgccgggaca agctggcaaa cagcagcagc
     3181 aacgaccccc tcattttcat attgtgcggt gaccataaat aatgcttttc tttctatcgg
     3241 cacagacgtg tgggcttcaa acgatggata cacctgggac tacgtatcat cgccattcgg
     3301 gtttacgcga gagggtgcgg cctcgaatac tgatggcaga gtaatcgccg ttggagaaac
     3361 acctgaaggt tcgttttatt ctgatgactc cggagtgtct tggacactcg tcgatccatc
     3421 ggcgacatcg aatctgatcg gaataacatt cgggaaaggc atttgggtta ccgttggagc
     3481 agactccact acgatgatag acggcgtgat gcactactcc acagacaacg ggtcatcatg
     3541 gtcgttcaat gtcgcgcccg catcgccggt tttacgctgc gtcgcctacg ggtaccgcta
     3601 atcacagaac acgcgtgggg tatatgttaa ggcgaacatc cactaatctg tttgttggca
     3661 tgaccgcgaa aaccctgatc attcttggcg tactttgtgc gccggcctgc cagcaggaga
     3721 acctatggga gcttgcatca aaggactcac gccgagaggg gattttcgtg gccgtgggcg
     3781 aatcgggtgt cgttattaca tcacgcgatg gcttgcaatg gacgccgcaa ccgtcgatag
     3841 tcaataccca cttgatcggg ata
//
LOCUS       DAORHF010000319         3861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_25828_length_3861_cov_4.952181, whole genome shotgun sequence.
ACCESSION   DAORHF010000319 DAORHF010000000
VERSION     DAORHF010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25828_length_3861_cov_4.952181"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /gene="aspS"
                     /locus_tag="PKJ16_17560"
     CDS             <1..1486
                     /gene="aspS"
                     /locus_tag="PKJ16_17560"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HNV48855.1"
                     /translation="VADEIVILNASVAPPIPIDSRENVNEEQRLAYRFLDLRRADMQE
                     AIIVRHRVMQAVRAYCAANRFIEIETPMLNKSTPEGARDFLVPSRLSRGMFYALPQSP
                     QLFKQILMVSGFDRYFQIVKCFRDEDLRNDRQPEFTQVDLELSFITRDMIIDIIEGMI
                     AVIVREGFGKEVSLPFPRITYDDAMRRYGKDAPDTRFGMELSELSDLFAQSSFNVFSS
                     TVASGGIIKAFVVEDKDRLSRKNLDDYTEYVKVFKAGGMPWVRFRNGAFEGGIAKFLS
                     EPEREGLRARLSLRENAVVIFGCDRAEIVNATLAGMRLKIAQDLGMIDPELLNFVWVT
                     DFPLLEYDPGEKRYYAKHHPFTAPAAEHVAALDAITPETVDGITAQAYDIVLNGVEIG
                     GGSIRINTPALQKKMFELLGIGEEEARVKFSFLLDALQYGAPPHGGIALGLDRVMMLL
                     LKRASIRDVIAFPKTQKGQCLMSQAPSPVAPQQLKELSIKILER"
     gene            1488..2456
                     /locus_tag="PKJ16_17565"
     CDS             1488..2456
                     /locus_tag="PKJ16_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HNV48856.1"
                     /translation="MVERTFEIDDSSLCRRICGFRDRNIKAIEKILDVSIIPRGNTLI
                     VTSPREQGEKAMRLLHLMGDYLVVKDGTFEFDDFDIKYLAASLDRGTVVSAKDIERLK
                     IVIPESGRSIAPRTVNQANYITAIHRNPVTFSYGPAGTGKTYLAVAVALSYHLSGKAE
                     RIILTRPAVEAGENLGFLPGDLIQKINPYLRPLYDALFDLLPFERISGMMEKNIIEVA
                     PLAYMRGRTLNRAFIILDEAQNTTVSQMKMFLTRMGNNSKIVISGDITQIDLDKPKKS
                     GLLHAMKILKGIDEIGFIEFAKEDICRHPIVEKIVAAYERHTKSEA"
     gene            2490..>3861
                     /locus_tag="PKJ16_17570"
     CDS             2490..>3861
                     /locus_tag="PKJ16_17570"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48857.1"
                     /translation="MINFTDARRKINIYFRRPGIRNAFFYLLGIVVIATVLLSFNAIG
                     KTYTYTVGEISHDDIKVPYDIRYTIETETEKERQREVDGVALVFDRDQSVLVERLGIV
                     NALMNHVASTLKETPPIGTEDRTFQLILLKDTLPKYLQFGDDVLLELLRLENPAEFRK
                     ALSRIVIYLYERGILNEPYKNSLNLANKNVLIRPINASGEVKEITASLEDLWLPETAR
                     QNLLAVCKAIAPDLTAEQMRAAYQIVRSNLKPNLSFNLEETKKRVDEAAKRVKPATGV
                     LKKGLMIVREGDVITSDALENINLLNRHTTTANVSYIVGIFLIQLLFLLIFGYFLVSD
                     YSAILEDNKSSIVVFTLLLFFILFSFFVSRAENVHSSKFIFALVLPLSFVTMILVILY
                     NIFLALLVGVYMVFFTFVISGFSFPSLVLGFSSAMLGTFIVGNVEKRTDFHVAHNKGP
                     QHGRAEA"
BASE COUNT          758 a         1245 c         1144 g          714 t
ORIGIN      
        1 ggtcgccgac gagatcgtca tactcaacgc gtcggtcgcg ccgcccatcc ccatcgacag
       61 ccgtgagaac gtgaacgagg agcagcgcct cgcgtatcgc ttcctcgacc tcaggcgcgc
      121 cgacatgcag gaggccatca tcgtgcggca ccgcgtgatg caggccgtcc gcgcctactg
      181 cgccgccaac cgcttcatcg agatcgagac gcccatgctc aacaagtcca cgcccgaagg
      241 cgcgcgcgac ttcctcgttc ccagccgcct gagccgcggc atgttctacg cgctgccgca
      301 gtcgccgcaa ctgttcaagc agatcctgat ggtctccggc ttcgaccgtt acttccagat
      361 cgtgaagtgc ttccgcgacg aggacctgcg gaacgaccgc cagcccgagt tcacccaggt
      421 cgacctcgag ctttcattca ttacgcgcga catgatcatc gacatcatcg aggggatgat
      481 cgcggtcatc gtgcgcgagg gattcgggaa ggaggtgtcg ctgccgtttc cgcgcatcac
      541 ctacgacgac gcgatgcgcc gctacggaaa ggacgcgccc gacacgcgct tcgggatgga
      601 gctctcggag ctcagcgacc tgttcgcgca atcgtcgttc aacgtgttct cctccaccgt
      661 cgcctccggc ggcatcatca aggccttcgt cgtggaggac aaggaccgcc tgtcgcgcaa
      721 gaacctcgac gattacaccg agtacgtgaa ggtgttcaag gccggcggca tgccctgggt
      781 ccgcttccgc aacggcgcct tcgagggcgg cattgcgaaa ttcctgagcg agcccgagcg
      841 cgagggcctg cgcgcgcggt tgtcgctgcg cgagaacgcc gttgtcatct tcggctgcga
      901 ccgcgccgag atcgtgaacg cgacgttggc cggcatgcgc ctcaagatcg cgcaggacct
      961 gggcatgatc gatccggagc tcctcaactt cgtctgggtg accgatttcc cgctcctgga
     1021 gtacgatccc ggcgagaagc gctactacgc gaagcaccat cccttcacgg cgccggcggc
     1081 ggagcacgtc gcggcgctcg acgcgatcac ccctgagacg gtggacggca tcacggcgca
     1141 ggcctacgat atcgttctca acggcgtcga gatcggcggc gggtccatcc ggatcaacac
     1201 ccccgcgctg cagaagaaga tgttcgagct cctgggcatt ggcgaggagg aggcgcgcgt
     1261 caagttctca tttctgctgg acgcgctgca atatggcgcg ccgccccacg gcgggatcgc
     1321 gctgggcctg gaccgggtga tgatgctgct gctcaagcgc gcctcgatcc gcgacgtgat
     1381 cgcgtttccc aagacccaga agggccagtg cctgatgagc caggcgccct cgcccgtggc
     1441 accccagcag ctgaaggagc tttcgatcaa gattctcgaa cgctagtatg gttgagcgca
     1501 cattcgaaat cgatgattcg tccctgtgcc gcaggatctg cgggttccgg gaccggaaca
     1561 tcaaggccat cgaaaagatc ctggacgtga gcatcatccc ccgggggaac accctgatcg
     1621 tcacatcgcc gcgtgagcag ggcgagaagg cgatgcgcct cctgcacctg atgggcgatt
     1681 acctcgtcgt gaaggacggg accttcgagt tcgacgattt cgacatcaag tacctcgccg
     1741 cgtccctcga ccggggaacg gtcgtgagcg cgaaggacat cgagcggctc aagatcgtga
     1801 taccggagtc cggccgcagc atcgcgccgc gcacggtgaa ccaggcgaac tacatcacgg
     1861 cgatccaccg caacccggtg accttttcct acgggccggc cggcaccggc aagacctatc
     1921 ttgcggtggc cgtcgcgctg tcgtaccatc tttcgggcaa ggcggaacgg atcatcctca
     1981 cgcggccggc ggtggaggcc ggcgagaacc tgggctttct gcccggggac ctcatccaga
     2041 agatcaatcc atacctgcgc ccgctctacg acgcgctctt cgacctgctg cccttcgagc
     2101 gcatcagcgg catgatggag aagaacatca tcgaggtggc gccgctggcc tacatgcgcg
     2161 gccgcacgct caaccgggct ttcatcatac tggacgaggc gcagaacacc acggtgtcgc
     2221 agatgaagat gttccttacc cggatgggga acaactccaa gatcgtcatc tccggcgaca
     2281 tcacccagat cgacctggac aagcccaaga agtcgggtct gttgcacgcg atgaagatac
     2341 tcaagggcat cgacgagatc ggttttatcg agttcgccaa agaggacatc tgccgccatc
     2401 cgatcgtcga aaagatcgtc gccgcctatg agcggcacac caagagcgag gcctagcgac
     2461 ccgcacgcac gggcggggca gggacagcca tgataaactt caccgacgcg cgacgcaaga
     2521 tcaacatcta cttccgcagg cccggcatcc ggaacgcctt tttctatctg ctggggatcg
     2581 tcgtcatcgc caccgtgctc ctgtcgttca acgccatcgg caagacctat acctacaccg
     2641 tgggcgagat atcgcacgac gacatcaagg tcccgtacga catccgctac accatcgaga
     2701 cggagaccga gaaggagcgg cagcgcgagg tcgacggcgt ggcgctcgtc ttcgatcgcg
     2761 accagtcggt gctcgtggag cgcctgggca tcgtgaatgc gctcatgaac catgtcgcga
     2821 gcaccctcaa ggaaacgccg cccatcggga ccgaggaccg caccttccag ctcattttgc
     2881 tcaaggacac gctgccgaaa tatctgcagt ttggcgacga cgtcctgctt gagcttctgc
     2941 gcctcgaaaa tcccgccgag ttccggaagg cgctcagccg gatcgtcatc tatctgtacg
     3001 agcgcgggat cctcaacgag ccgtacaaga actccctcaa tcttgcgaac aagaacgtgc
     3061 tcatacggcc gatcaacgcc tcgggcgagg tcaaggagat caccgcgtcg ctggaggacc
     3121 tgtggcttcc ggagacagcc cgccagaacc ttctggccgt ttgcaaggcc atcgcgccgg
     3181 acctcacggc ggagcagatg cgggccgcct accagatcgt gcggagcaac ctgaagccca
     3241 atctgtcgtt caacctggaa gagaccaaga agcgcgtgga cgaggcggcc aagcgcgtga
     3301 agcccgcgac cggcgtcctg aaaaaagggc tcatgatcgt gcgcgagggc gacgtgatca
     3361 cctcggacgc gctcgagaac atcaaccttc tcaaccggca cacgaccacc gcgaatgtga
     3421 gctatatcgt gggcattttc ctgatccagc tcctgtttct gctcatcttc ggatattttc
     3481 tggtgtcgga ttacagcgcg atcctcgagg acaacaaatc gtcgatcgtg gtgttcacgc
     3541 tccttctgtt cttcatcctg ttctcgtttt tcgtgtcccg cgctgaaaat gtccacagct
     3601 cgaagttcat cttcgcgctg gtgctcccgc tgtcgtttgt gaccatgatt ctcgtgatcc
     3661 tgtacaatat cttcctggcg ctcctggtcg gggtgtacat ggtcttcttc acgttcgtga
     3721 tcagcggctt ctcgtttccg tcgctggtgc tgggcttcag ctcggccatg ctggggacct
     3781 ttattgtggg caacgtggag aaacggacgg atttccacgt tgcccacaat aaaggtcccc
     3841 agcatggccg agctgaagcc c
//
LOCUS       DAORHF010000320         3825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_26158_length_3825_cov_3.637135, whole genome shotgun sequence.
ACCESSION   DAORHF010000320 DAORHF010000000
VERSION     DAORHF010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26158_length_3825_cov_3.637135"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PKJ16_17575"
     CDS             <1..812
                     /locus_tag="PKJ16_17575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV48858.1"
                     /translation="EQRDTLDKLMGYSIVNSKGMLVPVREIVKVKEEVEPTKIWRTNG
                     RRCVSITAKIGRLSYEEAADRITAVMRDVSLPPEYAFEFDENIEKIRKSRSEMLVLIL
                     LAVLFVYMVLAVLFESLSIPLVIMVSVPLAVIGVLIVLFLTGSSLNMSVYIGLIILAG
                     TAVNNGCVLVDAMTINLRSAGSRSDRIAVIKETCLKHFRPVMATTITAIMGLIPALLN
                     TGEGSNLWRPLSLTVITGLSTSTALTLLVVPLMFNWMSRTIGLKTKITKKK"
     gene            831..1787
                     /locus_tag="PKJ16_17580"
     CDS             831..1787
                     /locus_tag="PKJ16_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48859.1"
                     /translation="MVKAHWGMFEQFAHAVTHIVCGLAFFLNKLIMIKTVTLIFGMML
                     VAAVLDLPAFAEPREVLADWERNADRTLWGEKIRTTVAESVLPGERAGYALNANCDEA
                     TIEAFGANRRLLQCELASDIRRGEITPRTVIMADQLVGYGAAGIAPMQDGERFAVRAV
                     YARTLSRGFSSRDLSASPKSSRNGEITLCGALVILNDNNHRVRVKLSSVTLKATDGRR
                     CAVCAENVNLFEYSLDFAKVDIFGTVLYLWEENPILDLRPFGHIPLVSFPVVQLSGTV
                     SLRNHQQPEMKNPIRRTIRADALCAHIGYAHRSPIISNEVFS"
     gene            1874..>3825
                     /locus_tag="PKJ16_17585"
     CDS             1874..>3825
                     /locus_tag="PKJ16_17585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48860.1"
                     /translation="MHGADVRFKTYQDDKSRFERSIDQAKDAAELEAWERVVEGGRDE
                     LRAAWERDADFKIAAALREEGDTPERRAALEQDRQEARAAWERDITAATDEAAGSWYA
                     RRQGITYERFDVERFFEALRDTEAIDRTQALADQVRDWDAIINPVAEEIRARWEESEE
                     AFLNALRARANDLDDARRDAYGREVARIEQEIERMFRLERNSYIYRERNKFISELLID
                     QQSLKRKSADEAAGSASERIINETLKDLEKEEERIRTGEMKTPEGAGAVDFSKLGENW
                     KEEIDRLLDAGLERWKRAQEELMNAMRNWKANAEDAYAAGDRMWREAYERLLGAQDQW
                     RTKIDREIQEGLADWQEQQLDLRDNIDLSRDNLLAYLQSQQEQWNENSRGLREMALYG
                     REAYRDARENELFLKDMYDRYLNSGYYEVAGYSRQCFPSDPCNNTPWGSVSIPIGNRY
                     GSNRLWYNVWFSQNDYDPATRTVVEHYRYSWYWNDDGGPDPWWKWHYWEYEWTNSINS
                     STPLEKRSRFYFYATTYDDWRSTASEYANIIATAEDYIHNRNMLGQSGGPGYLVNSDG
                     TYGLKYLMDGANIRGLENDQYLMTSAEFGYEYARRELDFRQKRLDIAEAIMQYAYPEE
                     YSSNPYTDILGRRESAETTVSRRDAA"
BASE COUNT          847 a         1020 c         1197 g          761 t
ORIGIN      
        1 aggaacagcg agacacactc gacaagctca tggggtattc cattgtaaac agcaagggca
       61 tgcttgtccc ggtcagggag atcgtgaagg taaaggagga ggtggagcca acgaagatct
      121 ggcggactaa cggacgccgc tgcgtttcaa ttaccgcgaa gatcgggaga ctttcatacg
      181 aggaggcggc ggacaggatc acggcggtca tgcgcgatgt gtccctaccg ccggagtacg
      241 ccttcgagtt cgatgaaaac attgagaaaa tcaggaaaag ccggtcggag atgctggtgc
      301 ttattctcct tgccgtcctg ttcgtgtaca tggtccttgc cgtcctgttc gagtcgttga
      361 gtattccgct ggtgatcatg gtttcggttc cgcttgccgt gattggtgtt ctcatcgtcc
      421 tttttctgac cggctcttcc ctgaacatgt cggtctatat tggccttatt atccttgccg
      481 gcaccgcggt gaacaacggc tgtgttttgg tcgacgccat gaccatcaat ctcaggtcgg
      541 cgggaagcag aagtgacaga atcgctgtaa tcaaggagac ctgcctcaag cacttccgtc
      601 cggtaatggc cacaaccatt acggcgataa tgggccttat tcccgccctt ctgaacaccg
      661 gtgagggaag caacctgtgg cggccgcttt cgcttaccgt gataaccggc ctttccacat
      721 ccactgctct aaccctcctt gtggtgcctc tcatgtttaa ctggatgagt cgtacgatag
      781 gcttgaagac gaaaatcact aaaaaaaagt aattgacacg ataaacggtg atggtaaaag
      841 cgcattgggg catgttcgag caatttgcac atgcggttac ccatattgtg tgcggtttgg
      901 cgtttttcct gaataagctt ataatgatta aaacagtcac attgatcttc ggtatgatgc
      961 ttgtcgcggc ggttctcgac cttcccgcgt tcgctgaacc gcgggaggtc ctcgcagact
     1021 gggagcgtaa tgccgaccga acgctgtggg gcgagaagat ccggacgacg gttgcggagt
     1081 ccgtgctccc tggcgagcgc gccggctatg cgctgaacgc gaactgcgat gaggccacca
     1141 tcgaggcctt tggcgccaat cgccgtcttc tccagtgtga acttgcatcg gacatccgtc
     1201 gcggtgagat aacgccacgc accgtcataa tggcggacca gctggtgggt tacggggccg
     1261 ccgggatagc gcccatgcag gacggggagc gtttcgccgt ccgggcagtc tacgcgcgca
     1321 ctctatcgcg cggcttctcc agccgggacc tgtcggcatc cccgaaatcc agccgaaacg
     1381 gcgagatcac gctgtgcggg gcgttggtca tcctgaatga taacaatcat agagtgcgag
     1441 ttaagctgtc ttctgtgacc ctgaaggcca ccgatggccg gcggtgcgcg gtgtgcgccg
     1501 agaacgtgaa tctcttcgaa tactcgctcg atttcgccaa ggtggacatc tttggcaccg
     1561 tgctctacct gtgggaggaa aatccaattt tggacctacg gccgtttggc cacatccccc
     1621 tcgtctcatt ccctgttgtt caattgtccg gcaccgtttc gcttcgaaat catcaacaac
     1681 ctgaaatgaa gaacccgatc cgaagaacca ttcgggcgga cgccctttgt gctcatatcg
     1741 gatacgccca tcgatcaccc atcatttcga acgaggtttt ttcatgaaga cagcgatcct
     1801 cccgaccctg atccggcgga tgtcatgtgt tgcattagtt gcagcgtgcg tgttcctgcc
     1861 gctgcaaggg cggatgcatg gcgcagacgt ccggttcaag acctaccagg acgacaagag
     1921 ccgcttcgag cgctctatcg atcaggcgaa ggacgccgcc gagctggagg cgtgggagcg
     1981 cgttgtcgag gggggacggg atgagcttcg ggcggcatgg gagcgcgatg ccgacttcaa
     2041 gatcgcggcg gcgcttcgcg aggagggtga cacgcccgag cggcgggcgg cgctggagca
     2101 ggatcggcag gaggcccgcg cggcctggga gcgggacatt acggcggcga cagacgaggc
     2161 ggcgggctcg tggtacgccc gtcggcaggg gatcacctat gagcgcttcg acgtggaacg
     2221 ctttttcgag gctctgcggg acaccgaggc gatagatagg acgcaggccc tcgcggacca
     2281 ggtgcgcgac tgggacgcaa taattaatcc ggtggcagag gagatccgcg cgcgctggga
     2341 ggagagcgag gaggcgttct tgaacgcatt gcgggcccgc gccaacgatc tcgacgacgc
     2401 gcggcgcgac gcctacggac gcgaggttgc gcgcattgag caggagatcg agcgaatgtt
     2461 ccggctcgag cggaacagtt atatttaccg cgagcggaac aagttcatct cggaactctt
     2521 gatcgaccag cagtcgttga agcggaaaag cgccgacgag gcggcgggaa gcgcaagtga
     2581 gcgcatcatc aacgaaacac tcaaggacct ggagaaagag gaggagcgga tacggactgg
     2641 cgagatgaag acgccggagg gcgccggagc cgtagatttc tccaagctgg gtgaaaattg
     2701 gaaagaagag atagaccgac tccttgacgc ggggcttgag cgctggaaac gcgcacagga
     2761 agagttgatg aacgcgatgc gtaattggaa ggcgaacgca gaggatgcct acgcggccgg
     2821 cgaccgcatg tggcgcgagg cctatgagcg gctccttggc gcgcaagacc agtggcgtac
     2881 aaagatcgac cgcgagatcc aggaagggct ggccgactgg caggagcagc agttggacct
     2941 gcgggacaac atcgacctgt cgcgggacaa cctgctggca tatttacaat cccagcagga
     3001 acaatggaac gaaaactccc gcggactcag ggagatggcg ctgtacggga gggaggcgta
     3061 tagagatgct cgggagaatg agctgttctt gaaagatatg tatgatcgct accttaatag
     3121 cggttattac gaggttgcgg ggtattcccg ccagtgtttt ccgagcgatc catgcaataa
     3181 tacaccctgg ggttctgttt ccatccctat cgggaatcgc tatggcagca atcgcttgtg
     3241 gtacaacgtt tggttttcac agaacgatta tgatcctgcc actagaaccg ttgtcgagca
     3301 ttatcgttac agctggtatt ggaatgacga tggcggcccc gatccgtggt ggaagtggca
     3361 ttactgggaa tatgaatgga ccaattccat aaacagcagc acgccgttgg aaaaaagatc
     3421 ccgcttttat ttctatgcga ccacatacga cgactggcgg agcactgcgt cggaatacgc
     3481 aaacatcatc gccaccgccg aggactacat ccacaaccgg aacatgctgg ggcaaagcgg
     3541 cggccccgga tacttggtca actccgacgg gacctacggc ctcaagtacc tcatggacgg
     3601 cgcgaacata cggggcctgg agaacgacca gtacctgatg acgtcggccg agttcggcta
     3661 cgagtacgcg cggcgcgagc tggacttccg gcagaagcgt ctggacatcg ccgaggcgat
     3721 catgcagtac gcgtatccgg aggaatactc gagcaatccc tatacggaca tcctgggccg
     3781 ccgcgagagc gcggagacga cggtgtcgcg gcgtgacgcg gcgca
//
LOCUS       DAORHF010000321         3784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_26580_length_3784_cov_4.861089, whole genome shotgun sequence.
ACCESSION   DAORHF010000321 DAORHF010000000
VERSION     DAORHF010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26580_length_3784_cov_4.861089"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..973
                     /locus_tag="PKJ16_17590"
     CDS             245..973
                     /locus_tag="PKJ16_17590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48861.1"
                     /translation="MPRKRKTTGTTLTGDSGRTVWKRRPSEIRGYQQALYANTDTGDF
                     LRVDFNLREKKARIYLEDHEEGGNPYFSVIIDGKVTIERNQTTGRAYDLNAKFSKRAD
                     HLATIPNREVLKLIRSNSGIGEGGEKRDEAKKRRKETLERTRKKYFRPEDEYGAESAR
                     RRGFRRFIGWWDVVDLVVGVGGAIGLYFYTFNFIYIGIALACFSLLVSFMDFFIRNRD
                     LMVVKTLLFFVAGAGLYVYGYYFR"
     gene            1080..1541
                     /locus_tag="PKJ16_17595"
     CDS             1080..1541
                     /locus_tag="PKJ16_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012750493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HNV48862.1"
                     /translation="MKKAESVASSLQVVCFKVGREEYGIDILKVQEILKVPKITVLPK
                     SAEYIKGVIDLRGKVIPIIDLGKRFGIEMGGDLRSSRVIVVDVKGKRIGLAIDAVSHV
                     AKIDAKDIEPPPPVVKGISGRYIAGIGKLGQDFVVILDIDQIFMIEELKNL"
     gene            complement(1538..2704)
                     /gene="larC"
                     /locus_tag="PKJ16_17600"
     CDS             complement(1538..2704)
                     /gene="larC"
                     /locus_tag="PKJ16_17600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HNV48863.1"
                     /translation="MNAALFDIQFGVSGDMLLGSLIDAGCDAGSLAAALGTLGLDGWT
                     MAPEKTTRHAITGTAARISARDDHAHRTLGDIERILTRSGLSESSKERARRVFARLAE
                     AEGRIHGIPASDVHFHEVGALDSIIDICAFCVALELMDIDTVHFGDFAFGTGTIQTRH
                     GEIPVPVPAVVDLCNGFRGLMTGRPGELTTPTGAALLTVLGTQRSLPFAGTVARQGYG
                     FGTRDYPFPSYTRVILMSEPAPPIETIVQMECSIDDMNPQLYPHVLERLFAAGALDAY
                     LAPVHMKKGRPGMLLTVIAREDATERLREIVYRETTTLGIRYARTAREKIEREFATVT
                     VKGCAIRIKIGKHNGEVLNIQPEYEDCKNAAEKIGIALKTVQALARLEYDRNPN"
     gene            complement(2701..3471)
                     /gene="larB"
                     /locus_tag="PKJ16_17605"
     CDS             complement(2701..3471)
                     /gene="larB"
                     /locus_tag="PKJ16_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HNV48864.1"
                     /translation="MDEKKLSELLDRFRAGELSRDDIIGHLRGLPFEDLSFAKVDHHR
                     ALRWGFPEVIFCRGKRPDQVALIAQQIHATGGNLLATRATPEMYEAVRERLPEATFDE
                     IAGTISCASAPPDPLPGRCLIITAGTSDLPVAHEARETARMLGLDASLIADVGVAGIH
                     RVLHFREELEAAAVIIVIAGMEGALPSVVAGLVTAPVIAVPTSIGYGTSFGGVTALLG
                     MLNSCVPGTAVMNIDNGFGAAFLAFRIISTQKRIGRTQ"
     gene            complement(3475..>3784)
                     /locus_tag="PKJ16_17610"
     CDS             complement(3475..>3784)
                     /locus_tag="PKJ16_17610"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV48865.1"
                     /translation="GVILPHIMEYNLTSSPGKYVHMSKVMDEDVKEITVIEAAIKAVE
                     GVRRMEIDVDIPQRLSQFNITKADFARAADIAVSYPLLDNAPRPLTKNEFETIMIAAF
                     "
BASE COUNT          732 a         1093 c         1243 g          716 t
ORIGIN      
        1 gatccggggt tttcgacatc tcgatgaaca actgaaacca atcattttaa tataggagta
       61 gcgtatgccc tgcgggagaa aaaaaaagag aaagaagatc gcgaatcata agcgcaaaaa
      121 acgccgacgg cagaatcgtc acaaaaagaa ataagggtcg attcggcagc catacatgag
      181 ttcctgaagg agatacgacg attggtgccg acacggcatc aatcgttttt tattgaaagc
      241 ggaaatgcca cgaaagagaa aaacaacagg caccacgctc accggcgatt cgggccgcac
      301 ggtctggaag cgccgtcctt ctgagattcg cggctaccag caggccctgt atgcgaacac
      361 cgatacgggc gatttccttc gggtggattt caatctgcgc gagaaaaagg cgcgcatcta
      421 cctcgaggac cacgaggagg ggggcaaccc gtatttctcc gtcatcatcg acggcaaggt
      481 caccatcgag cgcaatcaga ccaccggtcg cgcctacgac ctgaatgcga aattttcgaa
      541 gcgcgcggac cacctcgcga ccatccccaa ccgggaggtc ctcaagctca tccggtcgaa
      601 ttccggcatt ggcgagggcg gcgaaaagcg cgacgaagcg aagaagcgcc ggaaggagac
      661 gctcgagcga accagaaaga agtactttcg tccggaggat gagtacggag ccgagtccgc
      721 gcggcgacgc ggctttcggc ggtttatcgg ctggtgggac gtggtcgatc tggtcgttgg
      781 cgtcggcggc gcgatcggtt tgtatttcta taccttcaac tttatctata tcgggatcgc
      841 gctcgcctgc tttagccttc tggtgagctt catggatttc ttcatccgaa accgggatct
      901 catggtcgtg aaaacgctcc tctttttcgt cgcgggcgca ggcctgtatg tgtacgggta
      961 ttatttccga taaccgaaaa atgttcttga aatttccaat cgggcgaggg agtattacgg
     1021 cggcccccgg caacggcttc atcatgcgcg ggtgagcatc atgggaaacg ggaaccagaa
     1081 tgaaaaaggc tgaaagcgtc gcgtcatccc tccaggtggt ctgcttcaag gtcggccgcg
     1141 aggagtacgg tatcgacatc ctgaaggtgc aggagatcct caaggtcccc aagatcacgg
     1201 tgcttcccaa gtcggccgag tacatcaagg gggtcatcga ccttcggggc aaggtgattc
     1261 ccatcatcga tctcgggaag cgcttcggca tcgagatggg cggcgacctg cgcagcagcc
     1321 gggtcatcgt ggtcgacgtc aaggggaagc ggatcggctt ggccatcgac gcggtgagcc
     1381 acgtggccaa gatcgacgcg aaggacatcg agccgccgcc ccccgtggtg aagggcatct
     1441 cgggccgcta tatcgccggt atcggcaagc tggggcagga cttcgtggtg atcctcgaca
     1501 ttgatcagat cttcatgatc gaggagctta aaaacctcta gttcgggttt cggtcgtact
     1561 ccaggcgcgc cagcgcctga acggtcttga gcgcgatgcc gatcttctcg gcggcgtttt
     1621 tgcaatcctc gtactcgggc tggatgttca acacctcgcc attgtgtttt cctatcttga
     1681 tcctgatggc gcatcccttg acggtcacgg tcgcaaactc gcgttcgatc ttttcgcggg
     1741 cggtccgtgc gtagcggatg cccagggtcg tcgtttcgcg atatacgatc tcgcggagcc
     1801 gttccgtcgc atcctcgcgg gcgatcacgg tgagcagcat gccgggacgt ccctttttca
     1861 tgtggaccgg cgcgaggtag gcgtcgaggg ctccggcggc aaacaggcgc tccagcacat
     1921 gcggatacag ctgcggattc atgtcgtcga tcgagcattc catctggacg atcgtctcga
     1981 tgggaggcgc gggttcgctc atgaggatga cgcgggtata ggaggggaag ggatagtcgc
     2041 gcgtgccgaa gccgtatccc tggcgcgcga cggtgccggc gaacggtaac gagcgttgcg
     2101 ttcccagaac ggtcaacagc gcggcgcccg tcggggtggt gagttcgccg ggccggcccg
     2161 tcatcagccc ccgaaagccg ttgcacaggt ccacgaccgc agggaccgga acggggattt
     2221 ccccgtgtct ggtttgaatg gtgccggtcc cgaaggcgaa gtctccgaag tggacggtgt
     2281 cgatgtccat gagctcgagc gcgacgcaga atgcgcagat gtcgatgatg ctgtcgagcg
     2341 ccccgacctc gtggaagtgg acgtcggacg cgggtatgcc gtggatccgc ccctcggcct
     2401 cggcgagccg ggcgaacacc cggcgcgcgc gttccttcga tgattccgag aggccgctgc
     2461 gggtgaggat gcgttcgata tcgccgagcg tgcggtgcgc gtgatcgtcc cgggcgctga
     2521 tcctcgccgc ggtcccggtg atggcatggc gggtcgtctt ctccggcgcc atggtccaac
     2581 cgtcgaggcc gagcgttccc agcgcagcgg cgagcgatcc ggcatcgcac cctgcgtcga
     2641 tgagcgatcc gaggagcatg tcgcccgaga ccccgaactg gatgtcgaac agcgccgcgt
     2701 tcactgcgtg cgcccgatcc gcttctgcgt cgagatgatg cgaaaggcga gaaaggccgc
     2761 gccgaagccg ttgtcgatat tcatgacggc ggtccccggt acgcaggagt tgagcatgcc
     2821 caatagcgcc gtaacgccgc cgaacgaggt cccgtagccg atggacgtgg gtactgcgat
     2881 gaccggggcc gtaaccagac ccgcgacgac ggagggcagc gccccctcca tgccggcgat
     2941 tacgataatg accgccgctg cctcgagttc ctcgcggaag tgcaggaccc ggtggattcc
     3001 cgcgacgccc acgtcggcga tgagggaggc gtcgagcccc agcatgcgcg cggtctcgcg
     3061 cgcctcgtgg gccacgggga gatcggaggt gccggccgtg atgatgaggc agcgccccgg
     3121 aaggggatcg ggcggggcgc tcgcgcagga aatcgtcccg gcgatctcgt cgaaggtcgc
     3181 ctccggcagg cgctcgcgga cggcctcgta catctccggg gtcgccctgg tggcgagtag
     3241 attgccgccc gtggcgtgga tctgctgcgc gatcagcgcg acctggtccg gccgcttccc
     3301 ccggcagaag atgacctcgg ggaagcccca gcgcagcgcg cgatggtggt cgaccttggc
     3361 gaacgagagg tcctcgaagg gaaggccgcg gagatggccg atgatatcgt cgcgggaaag
     3421 ctctcccgcg cggaagcggt cgagtagttc tgatagtttt ttttcgtcca tgcgctagaa
     3481 ggccgcgatc atgatcgttt cgaattcgtt cttcgtgagc ggacgcggcg cgttgtcgag
     3541 caacggatag gagacggcga tatcggcggc gcgcgcgaaa tcggccttgg taatgttgaa
     3601 ctgcgagagt cgctgcggaa tgtccacgtc gatctccatg cgccgcaccc cttcgaccgc
     3661 cttgatggcc gcctcgatga cggtgatctc cttgacgtcc tcgtccatca ccttggacat
     3721 gtgcacgtac ttgccgggcg acgaggtgag gttgtactcc atgatgtgcg gcaggatcac
     3781 cccg
//
LOCUS       DAORHF010000322         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_26644_length_3778_cov_3.579103, whole genome shotgun sequence.
ACCESSION   DAORHF010000322 DAORHF010000000
VERSION     DAORHF010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26644_length_3778_cov_3.579103"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="PKJ16_17615"
     CDS             <1..442
                     /locus_tag="PKJ16_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48866.1"
                     /translation="VIGQRADGLRSSAWIARVRREEIEHAGAYRFLAPDGSWTGDIGA
                     AAALFGDVMGECSLSHHPGRGGYRIVYCRMFSNTIMMATFEDFASIGSMRTRAIVTLA
                     APEGSSGRPGWYYSGKEIFAAGDRLYVVCMHPFEYQPHLVRVRR"
     gene            complement(449..1039)
                     /locus_tag="PKJ16_17620"
     CDS             complement(449..1039)
                     /locus_tag="PKJ16_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV48867.1"
                     /translation="MDKSEKIRKKKKAIIEALKRCLARDVYSRITVQGIADEAGFSKG
                     GVLYYFPIKEQMYVELMEEMFRDIASDHERALKGNLKSDEKASISALYEVERFVLDRN
                     TIKILINLVMYGFEEEKIMQPIRMFLNRHLELYKSVINEARQDTPTRRKTDFEPDFIA
                     RIAQMTVLSAGFLESIEKTDFDSSKLIRYIISLFKG"
     gene            1293..3074
                     /locus_tag="PKJ16_17625"
     CDS             1293..3074
                     /locus_tag="PKJ16_17625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48868.1"
                     /translation="MKRNNRRTASIVIALVIGVAVHIAPDAARSQFLTDSASGYRDKD
                     LKRLPQSVARNIRAEVLRNVKGAVKITWAVHPESDDDFIVGRSTDMVDTPQRALKARS
                     VKLVPAGADSAVVDSNLRPGKYYYVIVSRDRVQEKDIKLYPDVNYTTVPVVIDEGGAY
                     VPADGSRYPDKVTLIHAMLVNRSQVLLTWKGVDGASVVYTVYRGSGPLNSPEKIRAAE
                     RLATVSDGSERFIDRAINRSGDYYYAVTTRDSAGNEDLQLVPYQSYTANGLYVDLKSL
                     SPVSGIQAELYGDRAVRIMWSGSGSSISGYRVYRSNEPINSRERLSRARLVGDSTMHR
                     TELFDRNLRNGSYYYAVLPLLGDGTTDTTLVERVNFTIDPVRIGTAARLALKSIRART
                     AGSGITVSWSVSGAPGTEKYILVRSTERLVSAADLEGGTFLRNVDISRGSYPDENPPV
                     GRLYYALVPENYASIKGFRLVSGRNVTAQPVSITGDDRDRDRAGTVTEESEDEYDATA
                     QSVDRVLKKYFYPGKYQLALQHLKPIAKKSESAFERAKAKLYIGRTLIEMRKYNRALD
                     YLLVRDVKKYFPEDSEFWSKYAIQRVR"
     gene            3156..>3778
                     /locus_tag="PKJ16_17630"
     CDS             3156..>3778
                     /locus_tag="PKJ16_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48869.1"
                     /translation="MRTKTIVGILLIVLGVALFLYYDRYLKPEREARILLTEGKIVFE
                     RGEESGDKIAINQAIGIFSKVIARYSDTKVVPEAYYYVGQCYEKLKLYKLAYLKYAYL
                     IKNQNDVLTKDMRKDVHMRIAHINILKNYSEEGVNRLYDLLNNSFDKDFRSRVYTELG
                     HAYLNQGSFQKAGKMFDISLAENGMNEESVLGKARAYKRMGRDAEADA"
BASE COUNT          776 a         1092 c         1158 g          752 t
ORIGIN      
        1 cgtcatcggg cagcgcgcgg acggattgcg ctcatcagcg tggatcgccc gggtgcggcg
       61 cgaggagatc gagcatgcgg gtgcgtaccg ctttctcgcg ccggatggct cctggacggg
      121 cgatatcggc gctgcggccg cgctctttgg cgacgtgatg ggcgaatgct cgctctcgca
      181 tcatcccggg cgcgggggat accggatcgt ctactgccgc atgttcagca atacgatcat
      241 gatggcgacc ttcgaagatt tcgcgtccat cggctcgatg cggacgcgcg cgatcgtcac
      301 gctggcggcg ccggagggat cgtcagggcg acccgggtgg tactattcgg gcaaggagat
      361 cttcgcggcg ggcgaccgcc tctatgtggt ctgcatgcat ccgttcgagt atcagccgca
      421 tttggtccgg gtgaggcggt gaccgtcatc accccttaaa aagggatatg atgtagcgga
      481 tgagcttcga cgagtcgaaa tcggtctttt ctatcgattc caggaaaccg gcgctcagga
      541 ccgtcatctg cgcgatgcgc gcgatgaagt cgggctcgaa atcggtcttc cggcgcgtgg
      601 gcgtgtcctg ccgcgcctcg ttgatgacgc tcttgtagag ctcgaggtgg cggttgagga
      661 acatgcggat cggctgcatg atcttttcct cctcgaagcc gtacatcacc aggttgatga
      721 ggatcttgat ggtgttgcgg tcgagcacga agcgctccac ctcgtagagc gccgagatgc
      781 tcgccttctc gtccgacttc aggttgccct tgagggcgcg ctcgtggtcg ctggcgatgt
      841 cgcggaacat ctcctccatg agctcgacgt acatctgctc cttgatgggg aagtagtaga
      901 gcacgccgcc cttggagaaa ccggcctcgt cggcgatgcc ctgcacggtg atgcgtgaat
      961 acacgtcgcg cgccaggcag cgcttgagcg cctcgatgat ggcctttttc tttttacgga
     1021 ttttttcgga tttgtccatt tctgtgttgt tacccctttt atatcaggaa cccgccatgc
     1081 gaatgccata ttatccgcat actatcataa ttttgttgca tttttccata gaggtcaatt
     1141 agttttttta taaatggtcg gttttatgcc ccaggtcgac tggggggcgc gggcgccggg
     1201 acgggacaaa ttaatgataa agggcgcacc tggcgttgcc gaaaattata aaagcgggtg
     1261 tgcatgcaca acagtggcgg attgataacc ctatgaagag aaacaaccgg cggaccgcat
     1321 caatcgtgat cgcgcttgtg atcggcgtcg cggtgcacat cgcgcccgac gctgcgcgct
     1381 cccagtttct cacggattcg gcgagcgggt atcgggacaa ggacctcaag cgcctcccgc
     1441 agagcgttgc gagaaatatc cgcgcggagg tcctgcgcaa tgtcaagggc gcggtgaaga
     1501 tcacctgggc cgtccatccc gagagcgacg acgatttcat cgttggacgt tccaccgaca
     1561 tggtggacac accgcagcgc gccctcaagg cccgctcggt caaactggtg cccgccggag
     1621 ccgacagcgc cgtcgtcgat tcgaacctgc gacccgggaa gtactattat gtgatcgtct
     1681 cgcgcgatcg ggtccaggag aaggacatca agctctaccc cgacgtcaat tacacgacgg
     1741 tgccggtggt catcgacgag ggcggcgcat atgtcccggc cgacggatcg cgctatcccg
     1801 acaaggtcac gctcattcac gcgatgctcg tgaaccgaag ccaggtgctg ctcacctgga
     1861 agggggtcga tggcgccagc gtggtctata cggtctaccg gggcagcgga ccgctcaatt
     1921 cccccgaaaa gatacgtgcg gccgagcggc tcgcgacagt ttccgacggc agcgagcgat
     1981 ttatcgaccg cgccatcaac cgctccggcg actattatta cgccgtcacg acccgcgatt
     2041 ccgcggggaa cgaggacctg cagctggtgc cgtatcagag ctacacggcg aacggattgt
     2101 atgtcgatct caagtcgctc agtcccgtgt caggcatcca ggccgagctc tacggcgacc
     2161 gcgcggtgcg catcatgtgg tcggggagcg gatcgtccat cagcggatac cgcgtatatc
     2221 ggagcaacga gcccatcaac agccgggagc gattgtcgag ggcgcgactg gtcggcgaca
     2281 gcaccatgca tcggaccgaa ttgttcgacc gcaatctgcg gaacggatca tactattacg
     2341 ccgtactccc gcttctgggc gacggcacca ccgatacgac gctcgtggag cgggtcaatt
     2401 tcaccatcga tccggttcgc atcggcaccg cggcccgtct tgcgctcaag agcattcggg
     2461 cgcgcaccgc cggaagcggc atcaccgttt catggtccgt ctccggtgcg ccgggcaccg
     2521 agaaatacat cctggtgcgc tccacggagc gcctggtgag cgccgcagac ctcgagggcg
     2581 gcacgttcct ccgcaatgtc gatatttcgc gcggatcgta ccccgacgag aatccgccgg
     2641 tcggtcggct ctactacgcg ctcgtccccg agaactacgc atcgatcaaa ggattccgcc
     2701 ttgtcagcgg ccggaacgtg actgctcagc cggtctccat aaccggggac gaccgcgatc
     2761 gcgaccgtgc gggaaccgtc accgaggagt cggaggacga gtatgatgcg accgcgcaat
     2821 cggtcgatcg tgtcctgaaa aagtactttt atccgggaaa ataccagctc gcattgcagc
     2881 acctgaagcc gatcgcgaaa aaatccgaaa gcgccttcga gcgcgccaag gcgaagctgt
     2941 acatcggccg cacgctcatc gagatgcgca agtacaatcg cgcgctcgat tatctgctcg
     3001 tccgtgacgt gaagaagtat tttcccgagg attcggaatt ttggagcaag tacgcgatac
     3061 agcgggtgcg ataaggcgcg gcgtttgtag tgaagataac ggaggcgccg gtcgataatc
     3121 tcattgagcg gaagccacgg caaggagaat gacccatgag gaccaaaacg atagtcggca
     3181 tactgttgat cgtgctcggc gtggcgctgt tcctgtatta cgaccgctac ctgaagcccg
     3241 agcgcgaggc gcgcatcctg ctcaccgagg gcaagattgt cttcgagcgc ggggaggagt
     3301 cgggcgacaa gatcgccatc aaccaggcca tcggaatctt ttcaaaggtc atcgcacggt
     3361 attccgacac caaggtggtt cccgaggcct actattacgt agggcagtgc tacgaaaaac
     3421 tcaagctgta caagctggcc tacttgaagt acgcctatct catcaagaac cagaacgacg
     3481 tattgaccaa ggacatgcgg aaagacgtcc atatgcggat cgcgcatatc aacatattaa
     3541 agaattattc ggaggagggt gtcaaccggc tctacgatct gctcaacaac tccttcgaca
     3601 aggattttcg cagcagggtg tacaccgagc tcgggcacgc ctatctcaat cagggcagct
     3661 tccagaaggc cggcaagatg ttcgacatct cgcttgccga gaacgggatg aacgaagagt
     3721 cggtgcttgg caaggcgcgc gcttacaagc gcatggggcg cgatgccgag gccgatgc
//
LOCUS       DAORHF010000323         3761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_26826_length_3761_cov_3.743659, whole genome shotgun sequence.
ACCESSION   DAORHF010000323 DAORHF010000000
VERSION     DAORHF010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26826_length_3761_cov_3.743659"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1315)
                     /locus_tag="PKJ16_17635"
     CDS             complement(47..1315)
                     /locus_tag="PKJ16_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48870.1"
                     /translation="MKKLMVLLTLAAVSLGTGCIHYVRGDLGYINEKLDANKEPKKIA
                     YRVNTSYYGAGLTYGDYEKEEKVIGKVVGDLFNKNVLYTEQTLKKKPENLFFDVSVMF
                     HQVSDEGDLMGGAVLAGLTFMLYPNWWSRYNIYFAVDVYDKNQVSTPEGAGIALGDNR
                     TFRPLARYEYQQAIVSRWGGVILWALVLPCIIANKDRALTRVITDMTKEFMCDLKDGR
                     KNIVSHNHWRGSRGLTLAPEQVGDYRKGGKYRDMLEAAAGGMMAASSAMNTYASMQQG
                     AKPGMSPAASMMNTTQAVLRITKDLSLVAKSASDIRTQKKMLAYKNDNPDMRFFATDG
                     DPFKYKGGVKLWWVTYKTSKTGDQQFLDYFSGIDDAKWSHNFMSRYPDKYQITSNLES
                     KTFGSPSAAVQFIRNKAKQIGDKRYMDIKL"
     gene            complement(1556..3001)
                     /locus_tag="PKJ16_17640"
     CDS             complement(1556..3001)
                     /locus_tag="PKJ16_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48871.1"
                     /translation="MKKLMVLLTLAAVSLGTGCIHYVRGDLGYINEKLDANKEPRQIA
                     YRVNTNFYSKLPPLGNGEAYYSGIGSTLNDVSKPIGDYNPLNIFSGQKGGLAKVEVTL
                     KCGHGDCPGNPWRFKYYQWGAQEKKEINKIVGDIFNGNVLYTEKTLGKKPENLFFDIS
                     VMFHEVTDAVEGMKQAMLGGCTLTLWPFRTNRWNVYYSIDIYDKSQVSTPEGADVALG
                     DNRTFKPIARYEYQHVVMSRYTLLASLVIIPHLVGTSGRALNRAITDMTKEFMLDLKD
                     GRKSIVSHNHWRGARGLTLSFEQVGDDKRAEARAMSQMTAAGLQAASGALNTYASMQR
                     GSQPGMSPGSQYVDATSALMRTTKDLGLVAKAATDYRTQKKIQANKNDNMDERLFATD
                     GDPFKYKGGVRLWWYTYKTSTTGDQQFLAYFAGVDVAASNLNMMMRNPSAYAITSKIE
                     TQTFGSPSAAVQFIRSKAKQIGDKRFVDIKL"
     gene            complement(3242..>3761)
                     /locus_tag="PKJ16_17645"
     CDS             complement(3242..>3761)
                     /locus_tag="PKJ16_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48872.1"
                     /translation="IDTAAAGMMAASSAMNTYASMQKGARPGTGMQPGMQYLANTTAV
                     LRTTKELSLVAKAAKDMRTEKKNLANKKDNMDERLFASEGNAFPNKGGVKLWWYTYKM
                     STTGDQQYLAYFTGPDVVSSNASMMVNSPNAYTLTSKIETQTFGSPSAAVQFIRNKAK
                     QIGDKRYMDIKL"
BASE COUNT          789 a         1089 c          923 g          960 t
ORIGIN      
        1 gaccttcgga agcccgtccg cgggacgcac gctctgttaa cttctattaa agctttatat
       61 ccatataccg cttgtccccg atctgcttcg ccttgttgcg gataaattgc accgccgcgg
      121 acgggcttcc gaaggtcttc gattccagat tggaggtaat ctggtattta tctggataac
      181 gactcatgaa attgtgactc cattttgcat cgtcaatacc ggagaagtaa tccaggaact
      241 gctgatcacc ggtcttgctc gttttgtagg tgacccacca tagctttacg ccgcccttgt
      301 acttgaaggg atcaccgtcg gtcgcaaaga accgcatgtc ggggttgtcg ttcttgtatg
      361 cgagcatctt cttctgggtg cgaatgtcgc tggctgattt tgcgacaagg ctcagatcct
      421 tcgttatacg cagtaccgct tgcgtagtgt tcatcattga agccgcaggc gacattcccg
      481 gcttcgcacc ctgctgcatt gaggcatagg tattcattgc gcttgaggcg gccatcatgc
      541 cccctgcggc ggcttccaac atatcgcggt atttaccccc tttacgataa tcaccgacct
      601 gctctggcgc caaggtcagc ccccttgatc ctcgccagtg attatgcgag acgatgttct
      661 tgcgaccgtc cttgaggtcg cacatgaatt ccttggtcat gtcggtgatc accctggtga
      721 gcgcacggtc cttgttcgcg atgatgcatg gaagaaccag cgcccacagg ataacaccgc
      781 cccaacgaga cactatggct tgctgatatt catagcgggc gaggggcctg aatgtccggt
      841 tgtccccaag cgcgatgccc gccccctcgg gagtggacac ctggttttta tcgtacacgt
      901 cgacggcgaa atagatgttg taccggctcc accagttggg atagagcatg aaagtcaaac
      961 ccgccaagac ggcgccaccc atgagatcgc cctcgtccga cacctgatgg aacattacag
     1021 atacatcgaa gaaaaggttt tcgggcttct tcttcagggt ttgttcggtg tataagacgt
     1081 tcttgttgaa caggtccccg acgactttcc cgatgacctt ttcctccttc tcgtaatcac
     1141 cgtaggtcaa accagcccca taataacttg tattgactct atacgcgatt ttcttgggct
     1201 ccttgttcgc atccagcttc tcgttgatgt agcccagatc gccccggaca tagtgaatgc
     1261 agccggtacc aagggacacg gccgcgagcg ttaaaaggac cataagtttt ttcatttgca
     1321 cattacccca cgtgatagta ttgcatagat tcgattcacg ttgtcattgc gagcgaaaca
     1381 atccaacgcc ggctcttcga gttgaattga aaatagtacg aacgtaatct cgtgccgctt
     1441 tgccagattg cttcgtcgct atcgctcctc gcattgacgg cgcgaacgga ctcgctcaat
     1501 taaacctcga tcgggcgccg ccacgttgcg cgcaatgacg acacccgagg ttttactaca
     1561 gctttatatc cacaaaccgc ttgtccccga tctgcttcgc cttgctccga atgaattgca
     1621 ccgccgcgga cgggctgccg aaggtttgcg tctcgatctt cgaggtgatt gcatacgccg
     1681 atggattacg catcatcata ttgaggttgc tagcggccac gtccactccg gcaaaatacg
     1741 cgagaaattg ctggtctcct gtggtgctag tcttgtacgt gtaccaccac aatcgtacgc
     1801 cgcccttata cttgaaggga tcgccgtcgg tggcaaacag acgctcgtcc atattatcgt
     1861 tcttattcgc ctggatcttc ttctgcgtgc gataatcggt cgccgccttc gccaccaaac
     1921 cgagatcctt cgtggttcgc atcagcgccg acgtggcgtc cacgtactgg gaacccggcg
     1981 acatacccgg ctgagagccc cgctgcatgg acgcgtacgt attcagggca cccgatgcgg
     2041 cctgcaaacc cgctgccgtc atttgcgaca tggcgcgcgc ctctgcacgc ttgtcatcgc
     2101 ccacttgctc aaaggaaagc gtgagcccgc gcgctccgcg ccagtggttg tgcgagacga
     2161 tgcttttccg gccgtccttg aggtccagca tgaactcctt ggtcatgtcg gttatcgcac
     2221 ggttgagggc tctgccgctt gtgccaacaa gatgcgggat aataaccaac gacgccagca
     2281 acgtatagcg cgacataaca acatgctggt attcatagcg ggcgatgggc ttaaaggtgc
     2341 ggttgtcacc cagagcgaca tcagctcctt ccggcgtcga aacctggctc ttatcgtaaa
     2401 tgtcgatgga ataataaaca ttccaccgat tagtacgaaa cggccacaac gtaagcgtac
     2461 acccgcccag cattgcctgt ttcatccctt caacagcatc ggtcacttcg tggaacatga
     2521 ctgatatgtc gaaaaagagg ttctcgggct ttttccccaa tgttttctct gtatacagca
     2581 cgtttccgtt gaaaatgtca ccgacgattt tattgatctc cttcttttcc tgtgcccccc
     2641 attgataata cttaaacctc caaggattac cggggcaatc cccgtgaccg catttcagcg
     2701 tcacctcaac ttttgccaac cctcccttct ggcccgaaaa aatatttaga ggattgtaat
     2761 ccccaattgg cttcgagaca tcatttagcg tgcttcctat tccagaataa tacgcctcgc
     2821 cgtttcccag tggaggcagc ttcgaataga aattcgtgtt cactctatac gcaatctgcc
     2881 taggctcctt gttcgcatcc agcttctcgt tgatgtagcc cagatcgccc cggacatagt
     2941 gaatgcagcc ggtaccaagg gacacggccg cgagcgttaa aaggaccata agttttttca
     3001 tttgcacatt accccacgtg atagtattgc atagattcga ttcacgttgt cattgcgagc
     3061 gaaacaatcc aacgccggct cttcgagttg aattgaaaat agtacgaacg taatctcgtg
     3121 ccgctttgcc agattgcttc gtcgctatcg ctcctcgcat tgacggcgcg aacggactcg
     3181 ctcaattaaa cctcgatcgg gcgccgccac gttgcgcgca atgacgacac ccgaggtttt
     3241 actacagctt tatatccata taccgcttgt ccccgatctg cttcgccttg ttgcggatga
     3301 actgcaccgc cgcggacggg ctgccgaagg tttgcgtctc gatcttcgag gtgagcgtat
     3361 acgcgttagg cgaatttacc atcatgctgg cgttgctgga taccacatca ggaccggtaa
     3421 aatacgccag gtactgttgg tcgccggtcg tgctcatctt ataggtgtac caccacagct
     3481 ttacgccgcc cttgttcggg aaggcgttcc cctcggaggc gaagagtcgt tcatccatat
     3541 tgtctttctt attcgcgagg ttcttctttt cggttcgcat gtccttcgcg gccttcgcaa
     3601 ccagactcaa ctccttcgtt gtgcggagca ccgcggtggt attcgccaga tattgcatgc
     3661 cgggctgcat ccccgtcccc gggcgcgcac ccttctgcat ggatgcatag gtgttcatgg
     3721 cgctcgaggc ggccatcatt cccgccgcag cggtatcaat c
//
LOCUS       DAORHF010000324         3741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27033_length_3741_cov_4.992404, whole genome shotgun sequence.
ACCESSION   DAORHF010000324 DAORHF010000000
VERSION     DAORHF010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27033_length_3741_cov_4.992404"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2838)
                     /locus_tag="PKJ16_17650"
     CDS             complement(<1..2838)
                     /locus_tag="PKJ16_17650"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HNV48873.1"
                     /translation="MRAPYRYMPLAALLVLALATAAPAQLLDLPMVKFDPALEWRSIR
                     TEHFWIHYHRGLEPDARRLAVIAERVHQTLTPLTKWRPYFRTDIVLVDNMDDANGFSI
                     PVPFNRVQLFLARPTMHESLNNYDNWLELLFTHEYTHTLNIDAVRGLPEASRYLLGRV
                     CFPNAFLPYWATEGNAVYHESRDSRFGRVNSTYTDMVMRTEALHNTLASITAASHMPR
                     RWPTGLVPYLYGGLFVQYLEDTRGRNRFADIFIENSYNLLPYSDNIIPFILPFIDTTA
                     ETVYRKTFRALWEDYRTHLSKKYEAQIASIRQAPVTRHALVSDSGHNTVLPRFSRDGG
                     DLYYVRMTNYDKRALMRYSLKNATTATLARVNAPGDIAVGKSGAVYLADLELYRSFSL
                     YSDVFRYTDGYRQMSRGLRAVSIDVSDDEHETVYVTAPGDRYSLVLADAALRERARII
                     DTSDLQISYARLSPDGGRIAFSFKDRSGNVDIAVYDRAAKAFTRITDDASIDLQPVWH
                     PDGARIIFSSDRSGAYNLYEYRLATGELVRLTNLLGGALWPAVSPDGSGIAFSAYGKN
                     GFDIALMPYPVASPPQKLPAKPMAPDYFLPPPSAPVRTSEPTEQPYTPLRSLFNPAWI
                     PMLYSDEIYNDTYDAWLGAFTMGTDALYRHFYALAAAGAGTQQRAAVSAMYLYSGLYP
                     NIMLQYNDDSLFYGHDGFPWRETNNFNVKRTMEKSGTVAVDVPFLSFYSSHDIVLAYT
                     YQRTYTDWFLPPTDERHYTDDLARVRVAYLFSNASRYAWSVSPEDGRSVSLSYDFYHR
                     GIGSDYSFSKARAEYAEYLPGIFRNHVTLLRLRGGAAFDNPDHLAPFNVGRFERGAQG
                     GVASDDDLFGLRGYPAGLIYGNRTASAALEYRFPAVQPDCGFSTLPVLLKDVWLTVFG
                     EYGNTWSGDPRLSDFRSSAGLELHVRIPLGYT"
     gene            3078..3539
                     /locus_tag="PKJ16_17655"
     CDS             3078..3539
                     /locus_tag="PKJ16_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48874.1"
                     /translation="MKSSHDFPNVNPKNPDGLKPSGKPYTVMVVEDKEFHRKQIVQIL
                     ESEQYKIVATAQNGKEAVEMYDKVADKLDLITTDLDMPVLDGYALLFELMRKKPQAKI
                     VFISDETSKGVVQDLIKMGAADFILKPIQRQRLLDRVKLALSRPKGEAVDL"
BASE COUNT          742 a         1042 c         1295 g          662 t
ORIGIN      
        1 ggtatagccc agggggatgc ggacgtggag ctcgaggccg gcgctcgagc ggaagtcgct
       61 caggcgcgga tcgccgctcc aggtgttgcc gtattcgccg aagaccgtga gccacacgtc
      121 cttgaggagg acggggaggg tgctgaaacc acaatcgggc tggaccgccg gaaatcggta
      181 ctccagcgcg gccgaggccg ttcggttgcc gtagatgagg cccgcggggt agccccgaag
      241 cccgaagaga tcgtcgtcgg acgcgacgcc gccctgcgcg ccccgttcga accggcccac
      301 attgaatggg gcgaggtggt cgggattgtc gaaggccgcg ccgccccgca ggcgcagcag
      361 ggtcacgtgg ttgcgaaaaa tgccgggaag gtactccgcg tattcggcgc gcgccttgct
      421 gaaggaatag tcggacccaa tgccccgatg atagaaatcg tacgaaagcg aaacgctccg
      481 cccgtcctcg gggctcacgg accaggcgta ccggctcgca ttcgagaaaa gataggccac
      541 gcggacgcgc gcgagatcgt cggtatagtg gcgctcgtcc gtcggcggga ggaaccagtc
      601 cgtataggtg cgttgatagg tgtaggcgag cacgatatcg tggctgctgt agaacgagag
      661 aaacgggaca tcgacggcaa ccgtgcccga tttttccatg gtgcgcttga cgttgaaatt
      721 gttcgtctcc cgccagggaa aaccatcatg cccataaaag agggagtcgt cgttgtactg
      781 gagcatgatg ttcgggtaga ggcccgaata gaggtacatg gcggacacgg cggcccgttg
      841 ctgcgtcccc gccccggcgg ccgcgagcgc gtagaagtgg cgatacaggg cgtccgtgcc
      901 catcgtaaat gcgcccaacc acgcgtcgta ggtgtcgttg tagatctcgt ccgaatagag
      961 catcgggatc cacgcgggat tgaacagcga gcgaagcgga gtgtacggct gctcggtcgg
     1021 ttcgctcgtc cggaccgggg cgctcggcgg tgggaggaag taatccggcg ccatgggttt
     1081 cgcgggaagc ttttgcggcg gcgacgcgac cggatagggc atgagcgcga tatcgaaccc
     1141 gtttttcccg taggccgaaa aggcgatgcc cgagccgtcg ggcgagacgg cgggccacag
     1201 ggcgccgccg agcaggttgg tgagccggac gagctccccg gtggccaggc ggtattcgta
     1261 gagattgtag gcgcccgagc ggtccgacga gaatatgatg cgggccccgt ccgggtgcca
     1321 gaccggctgc aggtctattg aggcgtcgtc ggtgatgcgg gtgaaggcct tcgcggcgcg
     1381 gtcgtagacg gcgatatcga cattgcccga gcggtccttg aacgagaacg cgatccttcc
     1441 gccgtcgggc gagaggcgcg catacgagat ctgcaggtcg cttgtgtcga tgatgcgcgc
     1501 gcgctcgcga agcgcagcgt cggccagcac cagcgagtag cggtcgccgg gcgccgtcac
     1561 gtatacggtc tcgtgctcgt cgtcggagac gtcgatggac acggcgcgca acccgcggct
     1621 catctggcgg tagccgtccg tgtatcggaa tacgtcgctg tagagcgaaa aggagcggta
     1681 cagctccaga tcggccagat agaccgcgcc cgatttcccg acggcgatgt cgcccggcgc
     1741 gttgacccgc gcaagcgtcg cggttgtcgc gttcttcagc gaatagcgca tgagcgctcg
     1801 cttgtcgtaa ttggtcatgc gcacgtagta gaggtccccg ccgtcgcggc tgaagcgcgg
     1861 aaggacggta ttgtggccgc tgtcgctcac gagcgcatgc cgggttacgg gcgcctgccg
     1921 gatcgaggct atctgcgcct cgtatttttt ggaaagatgg gtccggtagt cctcccagag
     1981 ggcgcggaag gttttgcggt acaccgtttc ggccgtcgtg tcgatgaatg gcaggatgaa
     2041 ggggatgatg ttgtcggaat acggtaaaag gttgtaggaa ttctcgatga atatgtccgc
     2101 aaaccgatta cggccgcggg tgtcctcaag gtattggacg aagaggccgc cgtagagata
     2161 cgggaccagc ccggtcggcc agcgccgcgg catatgcgac gcggcggtga tcgacgcgag
     2221 cgtgttgtgg agcgcctccg tgcgcatcac catgtccgta taggtgctgt tgacgcggcc
     2281 gaagcgcgag tcgcgcgact cgtggtagac ggcgtttcct tcggtcgccc aatagggcag
     2341 gaaggcgttg gggaagcaga cccggcccag gaggtagcgg ctcgcctccg ggagcccgcg
     2401 cacggcgtcg atgttcaggg tgtgcgtgta ttcatgggtg aagaggagtt cgagccagtt
     2461 gtcgtaattg ttcagggact cgtgcatcgt cgggcgcgcc aggaagagct ggacccggtt
     2521 gaaggggacg gggatggaga agccattggc gtcgtccatg ttgtccacga gcacgatatc
     2581 ggtccggaaa tagggccgcc atttcgtgag cggggtgagc gtctggtgca cgcgctcggc
     2641 gatcacggcg agacggcggg cgtcgggctc caggccccgg tggtagtgga tccagaaatg
     2701 ctcggtccgg atggagcgcc actcgagcgc gggatcgaac ttcaccatcg gcaggtcgag
     2761 aagctgcgcc ggggcggccg tcgcgagggc gaggacgagc aacgcggcca ggggcatgta
     2821 tcgatagggg gctctcatcg gttgtgcgat ggacggaccg tcccgttgcc tgcgggccgg
     2881 acagggggca agtgtcggcg ggacgcgccg cctcgtcaaa ccgttatttg cttgatctgt
     2941 cgggattttt ttgcttgcat ggcattcggc gatgcggtat cccatttttt atagccggga
     3001 catcataatg attgacaaaa tcgctttttt acatttgtta cgagtgccgc agaatctcca
     3061 atgcgtggtg caaggaaatg aaaagcagtc atgacttccc caacgtaaac ccgaagaacc
     3121 ccgacggcct caagccgagc ggcaagccct ataccgtgat ggtcgtggag gacaaggaat
     3181 tccaccggaa gcagatcgtg cagatcctcg agtccgagca gtacaagatc gtcgccacgg
     3241 cccagaacgg caaggaggcc gtcgagatgt acgacaaggt ggccgacaag ctcgacctca
     3301 tcaccaccga cctcgacatg ccggtgctcg acggctatgc gttgctcttc gagctgatgc
     3361 ggaaaaaacc tcaggcgaag atcgtgttca tcagcgacga gacgtccaag ggcgtggtgc
     3421 aggacctcat caagatgggc gccgcggatt tcattctcaa gcccatccag cgtcaacgcc
     3481 tgctcgatcg cgtgaagctg gcgctgtcac gcccgaaggg cgaagcggtc gatctgtaaa
     3541 aaaataaaga tccgagagga agggacatgg accccgaaat cgccgaaacc atcgcgaaat
     3601 ctcccgagct cgcccggaag taccagatcg cgctgcacta tacgaacggc gatgaggaca
     3661 aggccctcaa gatgctcgat ggcgcctacc aggatctcta tatcatcaaa gggcgattca
     3721 gctcgaccac gcaaaacggc c
//
LOCUS       DAORHF010000325         3725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27216_length_3725_cov_6.344687, whole genome shotgun sequence.
ACCESSION   DAORHF010000325 DAORHF010000000
VERSION     DAORHF010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27216_length_3725_cov_6.344687"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PKJ16_17660"
     CDS             complement(<1..255)
                     /locus_tag="PKJ16_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48875.1"
                     /translation="MIKRNALFAVCAMGVIVLLSSVALNAQRKVDERVLNPKSKRMLA
                     ALRSGSASYFKSISFTEFNQANIVDDKGPSLISVFGFDGIP"
     gene            436..1077
                     /locus_tag="PKJ16_17665"
     CDS             436..1077
                     /locus_tag="PKJ16_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48876.1"
                     /translation="MKTIMHCTPIAPILIVCVCAIALIGCASGGASLTKETFAQIQNP
                     AAKGYFFGTVTRTGDWGMETKVMKPVARFLVQSETGVMYDLEFPMTDENKKNAFIFEL
                     PAGSYTIKQFKTGGGTFDKVMAFMDSSGSAKKGKKKVASKPSKENVARPLEVGLDIST
                     GSMTYVGSIVVQQQGKIKTDQWSKSLAEDKRVLLNDFPKLSTLRVSDPYNIVR"
     gene            1461..2861
                     /gene="argH"
                     /locus_tag="PKJ16_17670"
     CDS             1461..2861
                     /gene="argH"
                     /locus_tag="PKJ16_17670"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HNV48877.1"
                     /translation="MAGGEKKLWGGRFEKPSADITERLSASIHFDARLYRHDIRGSIA
                     HANMLAKTGIISPAERDAIVAGLREIEREIDAGTFRFERSREDIHMNIEAALTERIGE
                     PGRKLHTARSRNDQVALDTRLYLRDAIDEIDARLARLITLLSDLAERHIDIVMPGYTH
                     LQVAQPVRFSQHLLAHAWALTRDRRRLAFARESANELPLGAGALAGVNYPTDREMVRD
                     ELGFAGITPNSMDTVSDRDFALDFLYCAAALGMHLSRFCEELVLWSSAEFNFVRLADG
                     VTTGSSIMPQKRNPDLAELIRGKSGRLYGNLIGLLATLKGLPLAYNRDMQEDKEPLFD
                     SADTVTLALEGMIEMLATMTVNADVMRAALYRNFSTATDLADYLAKKGVPFREAHEIT
                     GRVVAHCEKNGIDFFALSADALAKISPAFGPDAAALLSPETSPERKVSAGGTARESVL
                     RQITALRRLVDAPTHG"
     gene            2854..3549
                     /locus_tag="PKJ16_17675"
     CDS             2854..3549
                     /locus_tag="PKJ16_17675"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HNV48878.1"
                     /translation="MVDSLTGIELSSPIVIVMRLTLAMLAGFLIGFEREKHHQAAGVR
                     THMALALGASLVMLLSIFIPTIFYPDAPESDPGRLAAQVISGIGFLGAGAIFRFGFDV
                     RGLTTAASIWTTSGIGLAFGAGFYWLGVIGTAFLIIVLQVFEFVENFMVAKRYIRFLH
                     VTFRSDQYEANKVINRIKQTKLEIRQVSIKENVEEKTTELIVNCRISDDFSIRELFDN
                     IKSLGHIISIRID"
BASE COUNT          759 a         1220 c         1056 g          690 t
ORIGIN      
        1 cggaatgccg tcgaaaccga acacgctgat gagcgacggg cccttgtcgt cgacgatatt
       61 ggcctggttg aattcagtga acgatatcga tttgaaatag gaagcgctcc ccgaccgcag
      121 cgccgcgagc atccgcttgc tcttgggatt gagcacccgc tcgtcgacct tgcgctgcgc
      181 gttaagcgcg acagacgaaa gaagaacaat cacgcccatg gcgcacacgg cgaataatgc
      241 attccgcttg atcattgtga tacctcggtc acgttcagta tgtactggtt ttttaataat
      301 acaagacgga tgtgtaaaga ttttgtcaat aataattaca aatatgtttg acctttgcaa
      361 aataagctga gatatgtgtc gaatatgggg aggatcgcgc cccgactatc aacaacgcca
      421 ttttggagaa ttgaaatgaa aaccatcatg cattgcacgc cgatcgctcc catcctgatt
      481 gtctgcgtat gcgcaatcgc cctcataggg tgcgcatccg gcggcgcctc gctcaccaaa
      541 gaaaccttcg cccaaattca aaatcccgcc gccaagggct acttcttcgg gaccgtgacc
      601 cgaaccggcg actggggaat ggagaccaag gtcatgaaac ccgttgcgcg cttcctggtg
      661 cagtccgaaa ccggcgtcat gtacgatctc gagttcccca tgaccgacga gaacaagaag
      721 aacgcattca tcttcgagct tccggcgggt tcgtacacca tcaagcagtt taaaaccggc
      781 ggcggcacct tcgacaaggt gatggctttc atggactcgt ccggctcggc gaagaagggg
      841 aaaaagaaag tcgcctccaa gccttcgaag gagaacgtcg cccgcccgct cgaagtcggc
      901 ctcgacatct cgaccggcag catgacctat gtcggctcta tcgtcgtcca gcaacagggg
      961 aagattaaaa ccgaccagtg gtcgaagagc ctcgccgagg acaagcgcgt gctcctgaat
     1021 gacttcccga agctctccac cctgcgggtt tccgatccgt ataacatcgt tcgatagtcg
     1081 cgcttcagcg acgcttgcaa cgccaataga tcacgacgac ccccgcgcac cgggggtcgt
     1141 cgttttttct ttcgatcgtt cacgcattac tcgatgcatt cgcgggcatg caatccgtca
     1201 tcgtcatcgc aacatcacgc gttgcgccag gtatgcatgc catgcgggtt cgcacgttcc
     1261 gatcgcctcg cttcgctcgc gacgacatgg tccaaacaac acgcatactt ttttccacac
     1321 gaatgatccg gagcgcttcc cggccaacac tcccagcacg gaccggaaat gcgcgccggc
     1381 agcaggcgtc gacaaacgat tcctgaattc gtccggaaaa aacaattgac gaaacgcgtc
     1441 tcgcggccac aatcccgcgc atggcaggcg gcgaaaagaa actctggggc gggcgattcg
     1501 aaaagccctc ggcggacatc accgagcggc tctcggcgtc gatccatttc gacgcgcgcc
     1561 tctaccgcca cgacatccgc gggtccatcg cccacgcgaa catgctggcg aaaacgggca
     1621 tcatcagccc ggccgagcgc gacgcaatcg tcgcgggcct gcgcgagatc gagcgcgaga
     1681 tcgacgcagg gactttccgc ttcgagcgct cgcgcgagga catccacatg aacatcgagg
     1741 cggcgctcac cgagcggatc ggcgagccgg gccgcaagct ccataccgcg cgctcgcgaa
     1801 acgaccaggt cgcgctggac acgcgcctgt acctgcgcga cgcgatcgac gagatcgacg
     1861 cgcggctcgc gcggctcatc accctcctgt ccgatctggc cgagcggcat atcgacattg
     1921 tgatgccggg ctacacgcac ctgcaggtgg cgcagcccgt gcgcttctcc cagcaccttc
     1981 tcgcccacgc ctgggcgctc acgcgcgacc gacggcgcct cgccttcgcg cgcgaatcgg
     2041 caaacgaact cccgctgggc gcgggggcgc tcgcgggcgt caactatccc accgaccggg
     2101 agatggtgcg cgacgagctc ggcttcgccg ggatcacccc caactcgatg gacacggtaa
     2161 gcgaccgcga cttcgccctt gattttctct actgcgccgc cgcgctgggc atgcatcttt
     2221 cgcgcttctg cgaggagctc gtgctctggt cgagcgccga gttcaacttt gtccgcctgg
     2281 ccgacggcgt gacgaccggt tcgtccatca tgccgcagaa gcgcaatccc gacctcgccg
     2341 agctcatccg cggcaagagc gggcggctgt acggaaacct catcggcctc ctcgcgacgc
     2401 tcaaggggct gccgctggcg tacaaccgcg acatgcagga ggacaaggag ccgctcttcg
     2461 actcggccga caccgtaacg ctcgcgctcg agggaatgat cgagatgctg gccacgatga
     2521 cggtgaacgc cgacgttatg cgcgccgctt tgtatcgcaa tttctccacc gcgaccgacc
     2581 tcgccgatta tctcgcgaag aagggcgtgc ccttccgcga ggcgcacgag atcaccgggc
     2641 gcgtggtggc ccactgcgaa aagaacggca tcgacttctt cgcgctatcg gccgatgcgc
     2701 ttgcgaaaat ctcgcccgcg tttggacccg acgccgcggc cctcctctcc ccggagacat
     2761 cgccggagcg aaaggtgtcg gcgggcggca ccgctcgcga gtccgttctc cgacaaatca
     2821 ccgcgctgcg ccggctcgtc gacgccccca cccatggttg attcgctcac cggcatagag
     2881 ctgagctcgc cgattgtcat cgtgatgcgg ctcacgctcg cgatgctggc gggctttctc
     2941 atcggcttcg agcgcgagaa gcaccatcag gcggcggggg tccgcacgca catggcgctc
     3001 gccctcggcg cgagcctggt gatgctgctc tccatcttca ttccgacgat cttctatccc
     3061 gacgcgcccg aatcggaccc cgggcggctc gccgcacagg tgatcagcgg catcggtttc
     3121 ctaggcgcgg gcgccatctt ccgcttcgga ttcgacgtgc gcgggctcac caccgcggcg
     3181 tccatctgga cgacatccgg catcgggctg gcgttcggcg cggggttcta ctggctgggc
     3241 gtcatcggca ccgcatttct catcatcgtc ctgcaagtct tcgagttcgt cgaaaacttc
     3301 atggtcgcga agcgatacat ccgcttcctc cacgtgacgt ttcggagcga ccagtacgag
     3361 gccaacaagg tcatcaaccg catcaagcag accaagctgg agatacggca ggtttccatc
     3421 aaggaaaacg tcgaggaaaa gaccaccgag ctcatcgtca actgccgcat cagcgacgac
     3481 ttcagcatcc gcgagctctt cgacaacatc aagtcgctgg ggcacatcat ctcgatccgg
     3541 atcgattgag cgggcggcgg accatcatcg aatcagcgcg cggggaggcg tacgcacatg
     3601 gacattattc ttctcatcgg ggccggcttc aatatcgcgg gaggcatcgg aattttcgtt
     3661 tcacaatttg tcgaactccc gtacagcttc ccacgcgtga ccgctcccga ccgcgccgat
     3721 ccacc
//
LOCUS       DAORHF010000326         3722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27259_length_3722_cov_4.788110, whole genome shotgun sequence.
ACCESSION   DAORHF010000326 DAORHF010000000
VERSION     DAORHF010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27259_length_3722_cov_4.788110"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PKJ16_17680"
     CDS             <1..411
                     /locus_tag="PKJ16_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48879.1"
                     /translation="AVTAEERASIGTLDKLTRIRTEMKERDIKILLKIEYDGDFIYIE
                     VTNTAPIMTQDMNRIHEKRDEYRQYRMEGREYEFFINNIDTSDSGFGLGYAKIDSFLT
                     SMGLDADRSISIISANNTTVMLSIPVSALRERMG"
     gene            complement(521..1141)
                     /locus_tag="PKJ16_17685"
     CDS             complement(521..1141)
                     /locus_tag="PKJ16_17685"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV48880.1"
                     /translation="MKKTIAGMIIIAIFGSFALSCKYADCRIVKPNQVKKMKRFAMFP
                     LKSENETLGTEIANNFEMYLAGKSDAGTEFEFVERSQLQKLLKEQNLTMAGVVDNPSL
                     MAGKVKGVDAILIGTVRLKTLSEAGQTCGQQALGCISLGMYRPKTYAETSIYPASVSV
                     KLVDVQSGVVLAYLNYEKDSFSEAKFVGEEMAKAFYTRINALTAQR"
     gene            1422..1883
                     /locus_tag="PKJ16_17690"
     CDS             1422..1883
                     /locus_tag="PKJ16_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012609460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3015 family protein"
                     /protein_id="HNV48881.1"
                     /translation="MKKFLVIALAAVVAISVGTELFAGQNNYGCGLGSIIFKGKDGLL
                     SQTCAVTFNGTCANQTFGITTGTSNCEQFKGIVSNEKINKFVADNMDNLAKDIAKGNG
                     EYLNTLAVLMEVKEPVRGQFYGKLQNNFGKIYTSEKITHVDVLNNIEKVMN"
     gene            1931..>3722
                     /locus_tag="PKJ16_17695"
     CDS             1931..>3722
                     /locus_tag="PKJ16_17695"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HNV48882.1"
                     /translation="MRKHVMSPLFFLAALLSLCVPVSAARADDTVAYQNYLVDLAVKK
                     RLHDERYWHLLLHYKRGVFGLRSLVDDPKFFLAPNGKRDPRAELEATIRAFFQTPIIE
                     DEHPVCKFVARFTWLTEQLAIDEDRLPVKRCKKVDTMLANMKPKSTTLIFPTSHINSP
                     ASMYGHTLLIVETATRSKLLAYAINYSAVTGDENFGPLYALKGLFGFYPGYFSVLPYY
                     SKIAEYSDFDFRDMWEYPLTLTEAETRRMMLHVFEMDYIASDYFFFDENCSFKLLFLL
                     ELARPTVRLTDDYPFWTIPLDTIRAVHRTGLVDSAIYRPSQVNKIRHIASLMSEDERE
                     LAEAVFAGKRPMDDIVKGPLPLEGRIRTVDFLSEYVQYKYSKDDITKAEYSSLFIKTL
                     RARSELGRPELSEYSYVAPPEPTTGHESDRVNLGVGVRGAEFFQEIRYRPSYHALSDF
                     DGGYVRGGQIVLGDVAVRYYDTRNIFVLQYLNAIDIQSISARDRFITPISWKVNTGLF
                     QKVMSDGRDDLVFRLSGGAGAAWDIRVPGLIFFMADLEADAGYKLRYAYCAGVGASVG
                     VLNEVRGTWKYQLGLRYVYFGVGDVHGEFSA"
BASE COUNT          776 a         1073 c         1051 g          822 t
ORIGIN      
        1 gccgttaccg cggaggagcg cgcgagtatc gggaccctcg acaagctaac gcgcattcgc
       61 accgagatga aggagcgcga catcaagata ctgttgaaga tcgagtacga cggcgacttc
      121 atctatatcg aggtgaccaa taccgcgccc atcatgacgc aggacatgaa ccgcattcac
      181 gaaaagcgcg acgagtaccg acagtaccgg atggaggggc gcgagtacga gttcttcatc
      241 aacaatatcg acaccagcga ctcgggattc ggcctgggct atgcgaaaat cgacagcttc
      301 ctgacaagca tgggcctgga cgccgaccgg tccatctcca ttatttcggc gaacaacacc
      361 acggtgatgc tctccatccc cgtatcggcg ctgcgggagc ggatggggta gcggtgggga
      421 agcgtcggct tgacccgcgg cgctacaagc aggtgcgggt gggcataaaa aaggaccgcg
      481 actatcgcgg tcctttttct atacggccgt aagttccgga ttatctttgc gcggtcagcg
      541 cgttaattct ggtgtaaaat gcctttgcca tttcctcccc gacgaacttc gcttccgaaa
      601 aactgtcctt ttcgtaattc agataggcga gtacgacgcc ggactgaacg tccaccagtt
      661 taacggaaac ggatgccgga taaatggacg tctcggcata tgtttttggc cgatacattc
      721 cgagcgagat gcaccccagc gcctgctggc cgcaggtctg tccggcctcg ctcagggtct
      781 tgagacgcac ggtcccgatg aggatcgcat cgaccccttt caccttgccg gccatgagcg
      841 aaggattgtc cacgacgccg gccatggtca ggttttgttc cttgaggagc ttctggagct
      901 gcgaccgctc gacgaattca aattcggtcc cggcatcgct tttgcccgca aggtacatct
      961 cgaaattgtt ggcgatctcg gtccccaggg tttcgttctc gctcttaagc gggaacatcg
     1021 cgaagcgctt catcttcttg acctgattgg gcttcacgat tcggcagtcg gcgtatttgc
     1081 acgaaagcgc gaacgagccg aatatggcaa tgatgatcat gccagctatc gttttcttca
     1141 tagcgtactc cttgaaaata gtcggaaaca tattgtcgct acatgggaat gcggatttat
     1201 gatttgatcc aatcttgata tggggcgatc attttgtcaa tacattatgc taaccatgta
     1261 tcgatattca atgaagcatg atagtgctcg ctggcgaacc cggtttttca actgattgaa
     1321 acattataaa gttgttgaca tgttttcaaa ttctagccag tctgtatgac ccgttcggga
     1381 actgttatcg tacgggtaaa atcaattatc ggaggttttt catgaagaaa tttcttgtca
     1441 tcgctttagc cgctgttgtc gccatttccg ttggcaccga actgttcgcc ggtcagaaca
     1501 actacgggtg cggtctcggt tcgatcattt tcaaggggaa ggacggcctc ctttcgcaga
     1561 cctgcgccgt gaccttcaac ggcacctgcg cgaatcagac cttcggcatc accaccggca
     1621 cctcgaactg cgaacagttc aagggcatcg tctccaatga gaagatcaac aagttcgtcg
     1681 ccgacaacat ggacaacctc gcgaaggaca tcgcgaaggg caacggcgag tacctgaaca
     1741 ccctcgcggt gctcatggaa gtcaaggagc cggtccgcgg ccagttctat ggcaagctgc
     1801 agaacaactt cggcaagatc tacacctctg aaaagatcac ccatgtcgac gttctgaaca
     1861 acatcgagaa ggtcatgaac taggcgttcg gtactaactg taattggtat tgtaatcagg
     1921 gggatatgag atgcgaaaac atgtcatgtc ccctttgttt tttcttgcgg cccttctttc
     1981 gctctgtgtc ccggtgtcgg cggcgcgcgc cgatgacaca gtcgcctatc agaactacct
     2041 cgtggatctg gcggtcaaga agcggctcca cgacgagcgc tactggcacc tcctgctgca
     2101 ctacaagcgc ggcgtgttcg gactgcggag cctggtggac gatccgaagt tctttctcgc
     2161 ccccaatggg aagcgcgacc cccgcgccga gcttgaggcg accatccgcg cgtttttcca
     2221 gacgcccatc atcgaggacg agcatccggt gtgcaagttc gtcgcgcgct tcacctggct
     2281 cacggagcag ctcgctatcg acgaggaccg cctgccggtg aagcgctgca agaaagtgga
     2341 taccatgctg gcgaacatga agccgaagtc caccacgctc atctttccca cctcgcacat
     2401 caacagcccg gcgtcgatgt acggacacac gctgctcatc gtggagacgg ccacccggag
     2461 caagctcctg gcctacgcca tcaactactc cgccgtaacg ggggacgaga acttcggacc
     2521 gctctacgcc ctcaaggggc tcttcgggtt ttatcccggg tatttctcgg tgctgcccta
     2581 ctattccaag atcgccgagt acagcgattt cgatttccgc gacatgtggg agtatccgct
     2641 cacgctcacc gaggcggaga cgcgccggat gatgctccac gttttcgaga tggattacat
     2701 cgcgtccgat tacttcttct tcgacgagaa ctgctcgttc aagctgttgt tccttttgga
     2761 gctggcgcgc cccacggtgc ggctcaccga cgattatccg ttctggacca ttccgctgga
     2821 caccatccgc gcggtgcacc gcaccggcct ggtcgattcc gccatttacc gcccctcgca
     2881 ggtgaacaag atcaggcaca tcgcgtcgct gatgagcgag gacgagcgcg agctcgccga
     2941 ggccgtgttt gcgggcaagc gcccgatgga cgatatcgtg aagggcccgc ttcccctcga
     3001 ggggaggatc cggacggtcg atttcctttc ggaatacgtc cagtacaagt attcgaagga
     3061 cgacatcacc aaggccgagt actccagcct gttcatcaaa acccttcgcg cgcgaagcga
     3121 gctcggccgc ccggagctct cggagtattc ctatgtcgcg ccgcccgagc ccacgaccgg
     3181 tcacgaaagc gaccgggtca atcttggcgt cggcgtgcgc ggggcggagt tctttcagga
     3241 gatccgctat cggccgtcgt accatgcgct ctcggacttc gacggtgggt atgtgcgcgg
     3301 cgggcagatc gtgctgggcg atgtcgccgt gcgctactac gatacgcgca acatcttcgt
     3361 attgcaatac ctgaacgcga tcgacatcca gtccatctcg gcgcgcgacc gcttcattac
     3421 ccccatctcg tggaaggtga acaccggcct ctttcagaag gtgatgagcg acggtcgcga
     3481 cgatctcgtg ttccgcctca gcggcggcgc cggagccgcg tgggacatcc gtgtgccggg
     3541 cctgatattt ttcatggccg acctcgaggc cgatgcgggc tacaagctgc gatacgccta
     3601 ttgcgcggga gtgggcgcgt cggtgggagt gctcaacgag gtccgcggga cgtggaaata
     3661 tcagctgggg cttcggtatg tgtactttgg cgttggggat gtccatggcg aattctcggc
     3721 at
//
LOCUS       DAORHF010000327         3705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27451_length_3705_cov_4.665753, whole genome shotgun sequence.
ACCESSION   DAORHF010000327 DAORHF010000000
VERSION     DAORHF010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27451_length_3705_cov_4.665753"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="PKJ16_17700"
     CDS             <1..1726
                     /locus_tag="PKJ16_17700"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV48883.1"
                     /translation="RTQVRGDIVIPIDRSGMVHINWAGKYYQSFNHVPYYAFLEYPDV
                     RDAVYGIFEQANPTAWSERASLARILSVFEADYQKGDDGARRALWPKILAARQRIAEI
                     ERAYLAPMERQIEALNAQLATGDDPALRRQLAERQNELTAMTLVQQVENLRGHALLIG
                     LTATASHDIGPISLDNNYPMVGTYHTIVNMVLAGAYIVVAPAAVNYLLMLLVALVIAL
                     AVQRLSARLSVMVIAASFFVLNAAVILLFALGPIWVDQLGLNLALVLPSMAVAGIKFI
                     REESQKKFIKEAFAHYMAPGVIDQIIQNPDLLQLGGENRRLTIFFSDVAGFTSISERI
                     TPNELVTLLNEYLSEMSDIVMKYDGTVDKFEGDAVMAFYGAPYHFPDHAAKACLAAIE
                     MQERLVDMRAAWQKQNKDQLFVRIGMNTGDVVVGNMGSKTRMDYTVMGDAVNLAARLE
                     GVNKTYGTGIIVSEFTYREAKDSIEVRHLDRIRVKGKEEPITIYELVGRAGRLADPVR
                     ELLACFDDGLRLFNERNWKQARQRFRDGLRINPDDGPCRVYADRCAEFMVKPPSRNWD
                     GVYRFTTK"
     gene            1883..2134
                     /gene="yajC"
                     /locus_tag="PKJ16_17705"
     CDS             1883..2134
                     /gene="yajC"
                     /locus_tag="PKJ16_17705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HNV48884.1"
                     /translation="MSFVPIVLMIVIFYFLLIRPSQKKEKERKKMIEALQKGDKVITA
                     AGIYGVISAIKPEEGIVVLKVAEGTKIEFSRTAIQAKVS"
     gene            2166..2762
                     /locus_tag="PKJ16_17710"
     CDS             2166..2762
                     /locus_tag="PKJ16_17710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48885.1"
                     /translation="MLKRWYIVFFLVLLVCAVVGVSHAQDDGATPPSGTVEKKAAESA
                     EDLVADPDAETKPAIKKEAKKKAKETKAKKKPKKSVEGEADDASGREAARDEKGMDPS
                     EVPGLLDLTDAEFKYRRIPNIKLERPAPAAADRDLKDSPAGDGKAVKAKGKDSESGGR
                     TSDTVAKVILLLIIIAIFVLYRLRAGKGQSRVLRRYPK"
     gene            2829..>3705
                     /locus_tag="PKJ16_17715"
     CDS             2829..>3705
                     /locus_tag="PKJ16_17715"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48886.1"
                     /translation="MTRGLFYKLIIILFTVALGVVWIMPTVGEKTMEVSFTAGATAEQ
                     REAVRKRFSVPSYRVADESGAVAVTGINLNDAILNEVRSQFPGVDDAKLRPHWSEKYF
                     YAKKVNLGLDLQGGMQLVLAADYERMEKKSGRKLTDDDKKDITEQALEMIRNRIDAFG
                     VSEPSIRSKGAEAIEIQLPGVKDPDKVKKVFGFTGKVEYRLVDNEYSSRAMEWLKANY
                     KEEKLPLTSKEQFALLEKIAKGVNLPAKLELLFYFERVKGVKDIYPVYPIALQREVAL
                     EGSDISKAWIGNDEYG"
BASE COUNT          777 a         1090 c         1166 g          672 t
ORIGIN      
        1 gcgcacgcag gtccgcggcg atatcgtcat ccccatcgac cgcagcggga tggtgcatat
       61 caactgggcc gggaagtact atcagagttt caaccatgtg ccctattacg catttctcga
      121 gtatcccgac gtgcgcgacg ccgtgtacgg gatcttcgag caggcgaacc cgacggcatg
      181 gtccgagcgc gcgagtctcg cccgcatcct gtccgttttc gaagcggact atcaaaaagg
      241 ggatgatggc gcgcgtcgcg cgctgtggcc caagattctc gccgcgcgtc agcgcatcgc
      301 ggaaatcgaa cgggcgtatc tcgcgccgat ggagcgccag attgaggcgc tcaacgccca
      361 gctcgcgacg ggggatgatc ccgcgctccg gcggcagctc gccgagcggc agaacgaact
      421 cacggccatg accctggtgc agcaggtgga gaacctgcgc ggccatgcgc tgctcatcgg
      481 actcaccgcg acggcctcgc acgatatcgg ccccatttcg ctcgataata attatcccat
      541 ggtggggacc tatcacacca tcgtgaacat ggtgcttgcc ggcgcgtaca tcgtcgtggc
      601 gcccgctgcc gtcaactatc tgctcatgct cctggtcgcc ctggtcatcg cgctcgcggt
      661 gcagcggctc agcgcgcgac tttcggtgat ggtcatcgcg gcctcgtttt tcgtgctcaa
      721 tgccgcggtc atcctcctct tcgcactggg gccgatctgg gtcgaccagc tgggtctcaa
      781 cctggcgctg gtgttgccgt cgatggccgt cgcaggcatc aagttcatcc gcgaggagag
      841 ccagaagaaa tttatcaagg aagccttcgc gcactacatg gccccgggcg tgatcgacca
      901 gatcatccag aatccggacc tgctgcagct cgggggcgag aaccggcgcc tcaccatctt
      961 cttctccgac gtcgccggct tcacgtcgat ctccgagcgg atcaccccga acgagcttgt
     1021 gaccctcctg aacgaatacc tctccgagat gtcggacatc gtcatgaaat acgacgggac
     1081 ggtggacaag ttcgagggcg atgcggtgat ggccttctac ggcgcgccgt atcatttccc
     1141 cgatcacgcg gccaaggcgt gcctggccgc catcgagatg caggagcggt tggtggacat
     1201 gcgcgcggcc tggcaaaagc agaacaagga ccagctcttc gtgcgcatcg ggatgaacac
     1261 cggcgacgtg gtggtgggga acatgggctc caagacgcgg atggactaca cggtcatggg
     1321 cgatgcggtg aacctcgccg cgcgcctgga gggcgtcaac aagacctacg gcaccgggat
     1381 catcgtcagc gagttcacct atcgcgaggc gaaggacagc atcgaggtcc ggcacctgga
     1441 ccgcatcagg gtcaagggca aggaagagcc catcaccatc tacgagctcg tggggcgcgc
     1501 cggacggctg gccgatcccg tgcgggagct tctcgcgtgc ttcgacgatg gcctgcggct
     1561 gttcaacgag cgcaactgga agcaggcgcg ccagcgcttt cgcgacggtc ttcgcatcaa
     1621 ccccgacgac gggccgtgcc gcgtatacgc cgatcgctgc gccgaattca tggtgaagcc
     1681 cccgtcgcgg aactgggacg gcgtatatcg cttcaccacc aaataaaatc ggttgacaga
     1741 tgggtgtgtt gtatttaagt ggtgaacgat ttttccccca tggaggatgc gtgtgttaac
     1801 ctgtatgtca attgcgatcg cccagggcac cacgggcgca cctgccggaa gctcgtccgg
     1861 cgccagcggc tttggttcgc tgatgagttt tgttccgatc gttctcatga tagtgatatt
     1921 ctatttcctg ctgattcgtc cctcgcaaaa gaaggagaag gagcgcaaaa agatgatcga
     1981 agcgctccag aagggcgaca aggttatcac cgccgccggc atctacgggg tcatctcggc
     2041 catcaaaccc gaggaaggga tcgtcgtcct caaggtcgcc gagggaacga agatcgagtt
     2101 ttcccgtacg gcaatacagg cgaaggtttc ctaggacaag aaccgcgcgg gacccctgct
     2161 gaccgatgct gaaacgatgg tacatagtgt tcttcctcgt gctgcttgta tgtgcggtgg
     2221 tcggggtgtc ccatgctcag gacgacggcg caacgccgcc gtcgggtacg gtggagaaga
     2281 aggcggcgga atcggcagag gacctggtcg ccgaccccga tgccgagacg aagcccgcga
     2341 taaagaaaga agcgaagaag aaagcgaaag aaaccaaggc gaagaaaaaa ccaaaaaaat
     2401 ctgtcgaggg cgaggctgat gacgcctcgg ggcgcgaggc cgcccgggac gagaagggga
     2461 tggatccgtc cgaggtgccg ggccttctcg atcttaccga cgcggaattc aaatatcggc
     2521 gcatcccgaa catcaagctc gagcgtcccg cgccggcggc cgccgatcgg gacctgaagg
     2581 acagccccgc cggggacggc aaggccgtca aggcaaaggg gaaggattcc gagtccggcg
     2641 ggaggacgtc cgataccgtt gcgaaagtga ttttgctgct cattatcatc gcgatattcg
     2701 tgctctatcg gctgcgcgcc gggaaggggc agtcacgggt gttgcggcga tatcccaagt
     2761 gatgcgggcc gcgtgcggcg acgcatcctc catacatatc ccatgaccgt tcgcgcaaag
     2821 agggtttcat gacacgagga ctattctaca agctcatcat aattctgttt accgtcgcgc
     2881 tgggcgttgt gtggatcatg ccaactgtcg gcgagaagac catggaggtc tccttcactg
     2941 ccggggccac cgcggagcag cgagaggcgg tccgaaagcg cttttcggtc cccagctatc
     3001 gggtcgcgga cgagtccggc gcggtcgcgg tcaccggcat caatttgaac gatgcgatcc
     3061 tcaatgaggt gcgctcgcag tttcccggag tcgatgatgc gaaactccgt ccgcactggt
     3121 cggaaaagta tttttacgcc aagaaggtga atctgggcct cgacctgcag ggcggcatgc
     3181 agctggtgct cgccgccgac tacgagcgca tggaaaagaa gtccggccgc aagctcaccg
     3241 acgacgacaa aaaggacatc accgagcagg cgctcgagat gatccggaac cgaatcgacg
     3301 ccttcggcgt ttccgagccg tccatccgct ccaagggcgc cgaggcgatc gagatacagc
     3361 tccccggcgt gaaggacccc gacaaggtga agaaggtctt cggtttcacc ggcaaggtcg
     3421 agtatcgcct ggtcgacaat gaatattcat ctcgggcgat ggagtggctc aaggcgaatt
     3481 acaaggaaga gaagctcccg ctcacgtcga aggagcaatt cgcattgctc gaaaagatcg
     3541 cgaagggcgt caatctgccg gcaaagctcg aattgctctt ttacttcgag cgcgtcaagg
     3601 gcgtcaaaga catttatccg gtgtacccga tcgccctgca gcgcgaggtc gcgctcgaag
     3661 ggagcgacat ctccaaggcg tggatcggga acgacgaata cggcc
//
LOCUS       DAORHF010000328         3665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27866_length_3665_cov_5.665097, whole genome shotgun sequence.
ACCESSION   DAORHF010000328 DAORHF010000000
VERSION     DAORHF010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27866_length_3665_cov_5.665097"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1907)
                     /gene="recJ"
                     /locus_tag="PKJ16_17720"
     CDS             complement(<1..1907)
                     /gene="recJ"
                     /locus_tag="PKJ16_17720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HNV48887.1"
                     /translation="MIPIRDKSAAGHEWRIAEIDESRAESIAAASGINPFIARLMVGR
                     GVDDPAQAERFLSPRLSYLYSPFQLEGMHDAVSRLRRALESGERIGIFSDSDLDGLTS
                     LAVLHGLIARINPSIDIIMRYPVADETYGLTRAVVDEMIAAGVRLLVTLDSGIRDVEE
                     IALARAAGIDVIVCDHHHPDDRLPDAIIVNPKRHDCHYPFKDLAGVGVTFKLCCGVLM
                     SYRSGFQKPLLVLCELGDALVALSMTDGAVVSVESLDAQSARIRLPNIVPDDSACIVS
                     LGDRYGWVPSECVPGRRMFDLATLVRDVSDAGTVPAGDGLDALCRALGLERSMSDVPG
                     ALAALVSEIDFRKSEKLRNYIETTMPLVALGTIADIMPVIGENRIIVHHGLKNLPATS
                     HPGLAYMVGGLKSLSARNVSWNIVPSLNAPGRFGKTELTAKFFLERENAELGVILKEI
                     GSLNERRRAMVDEIFTSIVRDMSNAETDDTGVLFLVRNDTPDGLTGLVANRLSGHYQK
                     PVIVVSHQGATGLVKGSGRSSVACDFLSIVSSAQGFFEKFGGHEQAFGFTTRAESLPE
                     IEACIREATQSYIGRAPIVRIDCEIPIEVITTEFVGALSSLEPYGNKNEEPVFLSRGV
                     MVRDFLRIGKD"
     gene            complement(1888..2574)
                     /locus_tag="PKJ16_17725"
     CDS             complement(1888..2574)
                     /locus_tag="PKJ16_17725"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HNV48888.1"
                     /translation="MDRVLQLLAEGKSLEKIAELGDASVADVVALVENARDILLAHHK
                     VTTRKKVVIKKEAKAVTAESEDRPDSDDVDVAQLFSGAELSAIPFSSTLVMNTDGASS
                     GNPGPAGIGIVIFDRDGRQVGKVSSYIGKRTNNYAEYYALVKALKIAVYFKARELRVR
                     TDSELVVKQIKGEYKVKNDSIRSLYEEAMALKKMVPSFKIEHVMRTFNDKADYLAKKA
                     VEGSGDSDSR"
     gene            complement(2612..3325)
                     /locus_tag="PKJ16_17730"
     CDS             complement(2612..3325)
                     /locus_tag="PKJ16_17730"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HNV48889.1"
                     /translation="MNKEIIIMIELQRHWDAVVRARADRQSSEREIADTNAKISAYEK
                     EVNDAETGLKALKTNMRQLELELKNLDETVSRLESRQAHLKTEKELDALNAELSRAKS
                     DRGNLEESLIAMIDEIAENELALGKSREGLAALRSESEAAIERLRERILRFDALGEEH
                     RGQFAELLTGLSPPVRVRFQKMTESASGVALARIDGSSCGVCHFELPPYLVTDAGRDD
                     KIATCINCGRYLFIAAPSR"
     gene            complement(3318..>3665)
                     /locus_tag="PKJ16_17735"
     CDS             complement(3318..>3665)
                     /locus_tag="PKJ16_17735"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HNV48890.1"
                     /translation="QERLGLTFAVYVGDESRVIRTVGVINGAGGGLVERILDERRLDC
                     IVTGDIGYHHARAAMDRGAALIDAGHYGTERYLLAVLIRDLREFLGSRDSIAPEFVQS
                     KIDLNPFAVRLNE"
BASE COUNT          774 a         1122 c          989 g          780 t
ORIGIN      
        1 ttgtccttgc caatccgtaa aaaatcgcga accatcaccc cgcgtgaaag aaacacgggt
       61 tcttcatttt tattgccgta gggttcgagc gatgagagtg cgccgacgaa ttcggtcgtg
      121 atgacctcta tcggaatctc gcagtctatg cgcactatcg gcgcacgccc gatatacgat
      181 tgcgtcgcct cgcggatgca cgcttctatc tccggaagcg attcggcgcg cgtcgtaaag
      241 ccgaaggctt gttcgtgtcc gccgaatttc tcgaagaagc cctgcgccga tgatacaatc
      301 gacaagaaat cgcacgcgac cgagctcctg cccgacccct tcaccaggcc agtggcccct
      361 tgatgtgaga caacgattac cggcttctga taatgtccgg acaatcgatt cgcgaccagt
      421 cccgtaagtc catcaggcgt atcattgcgg accagaaaca gcactcccgt atcgtccgtc
      481 tcggcgttcg acatatcgcg aacgattgac gtaaagattt catcaaccat ggcccgacgg
      541 cgctcattga gcgatccgat ctccttcagg ataactccca attcggcgtt ttcgcgctca
      601 agaaagaact tcgcggtcag ttccgttttc ccgaagcgcc cgggggcatt gagggacggc
      661 acgatgttcc aactcacatt gcgcgccgaa agcgatttga ggccgccaac catgtatgcg
      721 agtcccggat gcgaggtcgc ggggaggttt ttgagtccat gatggacgat aatcctgttt
      781 tcgcctatca ccggcatgat gtcggcaata gtacccaggg ccaccaaggg catggttgtt
      841 tcgatgtaat tgcgcagctt ttctgatttc ctgaagtcta tctcagatac gagggcagcg
      901 agggcacccg ggacatcgct catcgaacgc tccaggccca gagctcggca caatgcgtcc
      961 agtccgtcac ctgcaggaac cgtgccggca tcgctgacat cgcgcacaag cgtcgcgaga
     1021 tcgaacatgc gccgccccgg cacgcattcc gaggggaccc atccatagcg atcgccaagc
     1081 gacacgatgc acgcagaatc gtcaggaaca atattcggca agcgaattcg cgccgattgc
     1141 gcatcgagag attcgaccga gacgaccgcg ccatcggtca tcgaaagagc gacgagcgca
     1201 tcgccgagct cacagagcac caggagcggc ttttggaagc cgctgcgata gctcatcagc
     1261 acgccgcaac agagtttgaa ggtcaccccg acgccggcaa ggtccttaaa tggatagtgg
     1321 caatcatggc gcttcgggtt aacgatgatc gcatcgggga gacggtcgtc gggatgatga
     1381 tgatcgcata cgatgacatc aatgccggcg gcgcgcgcca gcgcgatctc ttcgacatca
     1441 cgaatccccg aatcgagcgt aacgagcaaa cggacgccgg cggcgatcat ttcatcgacc
     1501 accgcccggg tgaggccata ggtctcatcc gcgaccggat aacgcatgat gatgtctatg
     1561 gacggattga tgcgagcgat cagaccatga agaacggcca gcgaggtcag tccgtccagg
     1621 tccgaatcgg aaaaaattcc gattcgttcc ccggactcga gcgcacgccg aagacgcgag
     1681 acggcgtcat gcatcccctc gagctggaac gggctgtata gatacgaaag tcgcggtgaa
     1741 agaaaccgct cggcctgcgc tggatcgtcg acaccgcgac cgaccatcag acgcgcaata
     1801 aacgggttga tcccggaggc agcggcgatc gattcggcgc gcgattcgtc aatctcggcg
     1861 atccgccatt cgtgcccggc tgcggattta tctcgaatcg gaatcacccg atccctcaac
     1921 cgcctttttt gcgagataat ccgccttgtc gttgaacgtg cgcatcacat gctcgatttt
     1981 gaacgagggc accattttct tgagcgccat ggcctcttcg tacagggagc gaatcgagtc
     2041 gttcttgacc ttgtactcac ccttgatttg cttgaccacg agctcggagt ccgttcgcac
     2101 cctgagctcg cgcgctttga aatacaccgc gatcttgagc gccttgacca gggcgtaata
     2161 ctctgcgtaa ttattggtgc gctttccgat gtatgatgac actttgccta cttgcctgcc
     2221 gtcgcgatcg aaaatcacga tcccgatccc tgccgggccc gggttcccgc tggaggcgcc
     2281 gtcggtgttc ataaccagcg tcgaggaaaa aggtatcgcc gagagttccg ctccggaaaa
     2341 tagctgggcg acatcgacat cgtcactgtc cggacgatcc tcgctctcgg cagtcaccgc
     2401 tttcgcttcc ttcttgatga cgaccttctt tctcgtcgta accttgtgat gggcgagcag
     2461 gatgtcgcgt gcgttttcga ctagcgcgac aacgtccgcg acgctcgcat caccgagctc
     2521 ggcaatcttc tcgaggctct tcccctcggc gagaagctgc aagaccctat ccatgcttaa
     2581 catcatatct gtatcttcca gtttttttac gtcatcgtga cggagcggcg atgaacagat
     2641 atcggccgca attaatgcat gtggcgattt tatcgtcccg cccggcgtcc gtaacaagat
     2701 acggcggcag ctcaaagtgg caaacgccgc acgatgaacc gtcgatgcgc gcgagggcaa
     2761 cgccgctcgc ggactcagtc atcttttgaa agcgaacgcg caccggcggc gacagccccg
     2821 tgagtagctc ggcaaactgc cctcgatgct cctcacccag tgcatcaaag cgcaaaatcc
     2881 gctcgcgcaa ccgttcgatc gccgcctccg attcgctcct gagtgcggca agtccctccc
     2941 ggcttttacc gagggctaac tcgttctcgg cgatctcgtc gatcatcgca atgagcgatt
     3001 cctcgagatt tccccggtcc gatttcgcgc gtgacagctc ggcgttgagc gcatcgagct
     3061 ccttttcggt cttcaggtgt gcctgacgcg actccagccg cgagaccgtc tcatccaaat
     3121 ttttaagctc aagctccaat tggcgcatat tggtcttgag cgctttaagg ccggtttccg
     3181 cgtcattgac ctctttttcg tacgcggata tttttgcgtt ggtatcggcg atctcgcgct
     3241 ccgatgactg ccgatcggcc cgcgcccgga ccacggcgtc ccaatggcgc tgcaattcta
     3301 tcataatgat gatttcttta ttcattgagc cgtaccgcaa acggattgag gtcgattttc
     3361 gattgcacga attccggcgc tatggaatca cgcgagccca ggaattcccg gagatcgcga
     3421 atgagcaccg caagcaggta tcgctcagtg ccgtaatggc cggcgtcaat gagcgccgct
     3481 ccccggtcca tcgccgcgcg tgcgtgatga tagccgatat cgccggtgac gatgcagtcc
     3541 aagcgccgct cgtcaagaat tcgctccacc agtccgcccc ctgcgccgtt gatcacgccg
     3601 accgtccgaa tcactcgcga ctcatcaccg acatataccg cgaaggtcag ccccaatcgc
     3661 tcctg
//
LOCUS       DAORHF010000329         3661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_27913_length_3661_cov_3.722962, whole genome shotgun sequence.
ACCESSION   DAORHF010000329 DAORHF010000000
VERSION     DAORHF010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27913_length_3661_cov_3.722962"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PKJ16_17740"
     CDS             complement(<1..371)
                     /locus_tag="PKJ16_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48891.1"
                     /translation="MIIQINGHTADFELENERTARDVAESVTHWVKERNLILMEVQID
                     GEPSVASETPDTPLDAIDTLNFIVQSQADVVISSLNEGIGYCDRALRFIARATEDAGA
                     RPDELERLAAGIDWLVSLLDTV"
     gene            complement(478..2505)
                     /gene="fliD"
                     /locus_tag="PKJ16_17745"
     CDS             complement(478..2505)
                     /gene="fliD"
                     /locus_tag="PKJ16_17745"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HNV48892.1"
                     /translation="MPITMGGLSSGVDTQGIIQKLMEVEARPIQQWEEDRNWYNKRKT
                     ALGAMSTVVRDLNGAASELYGFRASYHDKKALSSNTGIVDALAGKEAEEGVRKVEVVR
                     LAGSHKISTDPIDQEEKLPAGKFTFTINNEAHHINFRGGSLKALQERIIESVSDWVST
                     NYINTEGTKHIITIESKTTGRRGEINISGDKEFLKKIGLIKGVKSDDKEKVTLQFDEK
                     YFTAYTGEKKIDEQDGSFTVEGGGASVKVNGMLWREYVLPSEVPVKDDTLLSFNFAYT
                     ARPEEKDDALPYRMEIGPDEKINIKGIELKGYNVPRVRPLNKKPPKPGDDASGIGVVS
                     MDGGVRTEKLYPIDRKATGVQEIPVGRDFKDKKIAKIIVYANDGTSVISNGAFTTPLK
                     DKGFLEPKNEIKKAGDAELKVDGVPMVRDKNEGISDVIKGLTLNLRSASKETVTINIT
                     NDLDNAVKKIKAFVEKYNKYLEVTNELTKAERAEKAGDYKKNRAKNGLFIGDMGLLRL
                     ENSLKTAVTGAYPSRAEVPVKVFSQLGVSTGAVNAEWSTIKDGKLVMDEDALRKALKQ
                     NPEGAKDFFGSDTDGDNRIDNGMAFTVAGILKPYVMTGKNIIQAKMDLETASIKNTDE
                     KIERLKDHLRRHEDKLRTKFAAMEKALNNSKSQGKWLNQQMGGGDGEGKRR"
     gene            complement(2649..2996)
                     /locus_tag="PKJ16_17750"
     CDS             complement(2649..2996)
                     /locus_tag="PKJ16_17750"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HNV48893.1"
                     /translation="MEINKVANDGRNFAHPRYAAGSERPVKDQGEKDAAEQKPTLSRE
                     DMLAAVESLNSVSRSVNTRVSFHFNDDANRVVMKVRDGKTEEVIQEIPPRELIRLLTQ
                     IRETIGMFVDESR"
     gene            complement(3134..>3661)
                     /locus_tag="PKJ16_17755"
     CDS             complement(3134..>3661)
                     /locus_tag="PKJ16_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HNV48894.1"
                     /translation="RMQIQVEVSQLIEEVDRIATSSEFNRMKMLTGVYSKASRTGSMF
                     FHVGPNQNQRIKAFISTMSSKALNLTDPGGKKRSISTVGQANSMIGYIDLALDKLNRQ
                     RADLGAYYNRMENTIRALDRSYENMLAADSRIRDADMATEMVEFTKNQILIQSGTAML
                     AQANFKPQLILNLLG"
BASE COUNT          603 a         1106 c         1051 g          901 t
ORIGIN      
        1 acggtgtcca gaaggctcac cagccagtcg atccccgcgg ccagccgctc gagctcgtcg
       61 ggccgtgcgc ccgcgtcctc ggtggcgcgc gcgatgaagc gcagcgcgcg atcgcagtac
      121 ccgatccctt cgttcaggga cgatatcacc acatcggcct gcgactgtac aatgaagttg
      181 agcgtgtcga tggcgtccag aggcgtatcg ggcgtctcgc tggccacgga gggctcgccg
      241 tcgatctgca cctccatgag gatgaggttt cgctccttga cccagtgcgt gacggactcg
      301 gccacgtcgc gcgcggtccg ttcgttttcc aattcgaaat cggcggtatg cccgttaatc
      361 tgaataatca cggtcgtctc cgaaagggtg atatgaatac ctatcggcag gggaaggggg
      421 attctgtcgg attttactcg tcgaagggcg ccgggccgat gcgcgttcgc gtgatcgtca
      481 gcgccgtttc ccctcgccgt cgccgccgcc catttgctgg ttgagccatt ttccttggga
      541 cttcgagttg ttgagcgcct tttccatggc ggcgaacttc gtccggagct tgtcctcgtg
      601 gcgccgcagg tggtccttga gtcgctcgat cttttcatcg gtgtttttga tcgaggcggt
      661 ctcgaggtcc atcttcgcct ggatgatgtt cttgccggtc atcacgtagg gcttgaggat
      721 cccggcgacg gtaaaggcca tgccgttgtc gatgcggttg tcgccgtcgg tatcggagcc
      781 gaagaaatcc ttggcgcctt cgggattctg cttgagcgcc ttgcgcagcg cgtcctcgtc
      841 catcaccaac ttgccgtcct tgattgtgct ccactcggcg ttcaccgccc cggtggacac
      901 gcccagctgg gaaaacacct tgaccgggac ctcggcgcgg ctgggatagg cgccggtcac
      961 ggccgttttc aacgagttct ccagccgcag caggcccatg tcgccgatga agagcccatt
     1021 cttggcgcgg ttctttttat agtctccggc cttctcggcg cgctcggcct tggtaagctc
     1081 gttggtcacc tcgaggtatt tgttgtactt ctcgacgaag gccttgatct ttttcacggc
     1141 gttgtcgagg tcgttggtga tgttgatggt gaccgtttcc ttcgaggcgc tgcgcaggtt
     1201 gagcgtgagc cccttgatca cgtcgcttat tccctcgttc ttgtcgcgca ccatcggtac
     1261 gccgtccact ttcagttccg cgtcgccggc tttcttgatc tcgttcttcg gctccaggaa
     1321 gcccttgtcc ttgagcgggg tcgtgaaggc gccgttggag atgacggagg tcccgtcgtt
     1381 ggcgtacacg ataatcttcg cgattttctt gtccttgaaa tcgcgcccaa cggggatctc
     1441 ctggacgccc gtcgccttgc ggtcgatggg atagagcttc tcggtgcgca cgccgccgtc
     1501 catcgacacc acgccgatgc cgcttgcgtc gtcgcccggt ttgggcggtt tcttgttcag
     1561 ggggcggacc cgcgggacat tgtatccctt gagctcgatt cccttgatgt tgatcttctc
     1621 gtcggggccg atctccatgc ggtaggggag ggcgtcgtcc ttttcctccg gccgagccgt
     1681 gtaggcgaag ttgaacgaga gcagggtgtc gtccttcacg ggcacctcgg agggcaacac
     1741 gtattcgcgc cacagcatgc cgttcacctt cacggacgcg ccgccgccct ccacggtaaa
     1801 cgatccgtcc tgctcgtcga tcttcttctc gccggtgtag gcggtgaagt atttctcgtc
     1861 gaactggagg gtcaccttct ccttgtcgtc ggatttcacg cccttgatga ggccgatctt
     1921 cttgaggaat tccttgtcgc cggagatgtt gatctcgccc cgacgtccgg tcgttttcga
     1981 ctcgatggtg atgatgtgtt ttgtgccctc ggtgttgatg taattcgtcg agacccagtc
     2041 cgagaccgat tcgatgatgc gttcctgaag cgccttgagc gagccgcccc ggaaattgat
     2101 gtggtgcgct tcgttattga tggtaaacgt gaactttccg gcagggagct tctcttcctg
     2161 gtcgatgggg tcggttgaga tcttgtggct gcccgcaagg cgcaccacct ccaccttccg
     2221 gaccccctcc tctgcttctt ttccggccag ggcgtccacg atgcccgtgt tcgaggagag
     2281 ggcctttttg tcatgatacg aggcgcgaaa accgtacaat tcgctcgcgg cgccgttgag
     2341 gtcgcggacg accgtgctca tggcgccgag agcggttttc cttttattgt accagttgcg
     2401 gtcctcctcc cattgctgga tgggacgcgc ctcgacctcc atgagcttct gaatgatgcc
     2461 ctgggtatcg actcccgatg aaagcccgcc catggtgatt ggcatgagat cctccccgat
     2521 gccgggacac tatacgcatg ttgcgcttac gcaagctgca tacaatgtat caacggcata
     2581 caatctcttc aatagaatat cggcaaaaag ccgttttttc gaaagacgcc aatcgaaaaa
     2641 aacgcgaatt accgcgattc gtccacgaac atgccaatcg tctcccggat ctgcgtgagc
     2701 agccggatca attcgcgcgg cggaatctcc tggatcacct cctcggtctt tccgtcgcgc
     2761 accttcatga cgacgcgatt cgcatcgtcg ttaaagtgaa acgacacgcg ggtgtttacg
     2821 ctccgggaca ccgaattgag gctctcgacc gccgccagca tgtcctcgcg cgaaagcgtg
     2881 ggtttctgct cggcggcgtc cttttccccc tggtccttca cgggccgttc cgagcccgct
     2941 gcataacgcg gatgcgcgaa attccttcca tcatttgcga ctttgttgat ttccatacct
     3001 acctccttgt agccggcccg gcttgtgtcg ggccaccgta cgtcttccct ccgtgaaaaa
     3061 aatccccctc cccgtttccc aggcggggag ggtaaaaccg accgcactgc agaagccggt
     3121 tttcatgaga tccctatccg agaaggttta agatcagctg aggtttgaaa ttcgcctgag
     3181 ccaacatcgc agtaccactc tggataagaa tctggttctt tgtgaactca accatctcag
     3241 tcgccatatc cgcgtcacgg atgcgggagt cggccgccag catgttctcg tacgagcggt
     3301 caagcgcccg aatcgtgttc tccatccggt tgtagtaggc ccccagatcc gccctctgtc
     3361 gattcagctt gtccagggcc aaatcgatgt atccgatcat ggagtttgcc tgccctacgg
     3421 tggagatcga ccgtttcttg ccgccggggt ccgtcagatt gagcgccttg gagctcatcg
     3481 tcgagatgaa cgccttgatg cgctggttct gattggggcc cacatgaaaa aacatgctcc
     3541 cggtccggct cgccttggag tagacgcccg taagcatctt catccggttg aactcggagg
     3601 aagtggcaat gcggtccacc tcctcgatca actgcgagac ttcaacctgg atctgcatcc
     3661 t
//
LOCUS       DAORHF010000330         3622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28385_length_3622_cov_4.608915, whole genome shotgun sequence.
ACCESSION   DAORHF010000330 DAORHF010000000
VERSION     DAORHF010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28385_length_3622_cov_4.608915"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1365)
                     /locus_tag="PKJ16_17760"
     CDS             complement(<1..1365)
                     /locus_tag="PKJ16_17760"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNV48895.1"
                     /translation="MTNSDRTKTVIRTAIAITIGLLLLAPLGYFLTVYFVSHEEIRTI
                     KPFNPALSTKLYDAHGVLISELYDEKRSYADLSAIPSVVRAAFLSAEDRGFYRHRGID
                     PLSVLRALVVDIAAGEIRQGGSTITQQLVKQLYTGRERTIRRKILELLLAGEIEKKFT
                     KDRILEMYLNQVYFGHGVYGVQSAARFYFDKDARALTPIEAAVLASIPAAPRRFSPLV
                     NPRNVYEKSKGILYTMIQDGHVEKSTVNRDFDGFWATFLASLRLQSPTASVRQRGSDR
                     APQVTEHVRRLLVAQYGEETVYRGGLSVYTTFDLNAQDIAQRTLSEGVERQRTIAARV
                     NAWKLAKLEKEQIAKRTKAAGNAHAAINAVRRDLSDTIILLSDVLGVSSIAQVAEDDS
                     SHNEEFIRSSRVEGALISIDPRTGAIVAVVGGSDTDDANQLNRAFQSLRQPGSAFKPF
                     VYGAG"
     gene            complement(1352..2143)
                     /locus_tag="PKJ16_17765"
     CDS             complement(1352..2143)
                     /locus_tag="PKJ16_17765"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV48896.1"
                     /translation="MFGFALENLTRALTLNDTLTDAANMIGDIYARRHKIKKAIRYFN
                     RSLEIKPEQDDIHVRIGALYDTIGKFDESRSHYRRAVDINRNNMGAQFNLIRSYVGAG
                     DRTSARASFNACSALCREEGSRIMNLGIELEKAKKMDRALAAYHQSMEVCPTLADAYF
                     AAAEAYRRTKKISKAAAIIDRLLSIDPENERALVYMAHLLFDNRDRAFRARHIRHTMK
                     CLQAALAINPKSVASHLLLSDVYRFTGDSLKADQHERKAREHDEF"
     gene            2306..2378
                     /locus_tag="PKJ16_17770"
     tRNA            2306..2378
                     /locus_tag="PKJ16_17770"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2339..2341,aa:Phe,seq:gaa)
     gene            complement(2404..>3622)
                     /locus_tag="PKJ16_17775"
     CDS             complement(2404..>3622)
                     /locus_tag="PKJ16_17775"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNV48897.1"
                     /translation="IDSVLKSDYNAQQRILIKNHVKQRLGFRAADPRVVAITTQLIPW
                     AVMERLDAEEAARFIVAFEGAISAGIEFTSAEDLIPALSRRKPATDDFILYSLYNRET
                     TRAGVPEHVRAAFLTRALDAGFDALSILAGGRGLICAHLISANTGDVSQRLLRSIPRA
                     GAKVPPGGFSRIIFTALHADVSKVTYERINEILENLVTIQRIHVGQSRARSDLQRIVT
                     TAQKIDSRIRSLSRSDEHRDGPIEPDTPYHHDEETPPEKLRPEEPAIDRWKRLRIDSL
                     QSAIKPWIGVPYKWGGLSKSGIDCSGFTRMVLTDRLIGVPPKLVPRSSLGQHRIQAGT
                     ALSSTTAYRAGDLLFFSASPDRKKITHVGLAVSRERFYHASSKGVIADDIDGKHWKPR
                     RIGGRRLFTDVVR"
BASE COUNT          773 a         1006 c         1018 g          825 t
ORIGIN      
        1 gcccgcaccg tacacaaacg gcttgaatgc cgatcccggc tgacggagcg attgaaaggc
       61 gcggttgagc tggttcgcgt catcggtgtc gctcccaccg acaacggcga caatcgcacc
      121 ggttcttgga tcgattgaaa tgagcgcgcc ttcgacacgt gacgatcgaa tgaattcttc
      181 attgtgcgaa ctgtcatctt ccgcgacctg cgcaatggac gaaacgccca gcacatccga
      241 caggaggatg atggtgtccg aaaggtcccg acggaccgcg ttgattgcgg cgtgtgcatt
      301 ccccgccgct ttcgtacgct tggcgatctg ttccttttca agttttgcga gtttccatgc
      361 gtttacccga gccgcgattg ttcgctgtcg ttcgacacct tcggacaatg ttcgctgggc
      421 gatatcctgc gcattgagat caaaggtcgt gtagacagaa agaccgccgc ggtatacggt
      481 ctcctcaccg tactgcgcga cgagcagtcg cctgacatgc tcggtcacct gcggcgcgcg
      541 atctgaaccg cgttgacgaa cgcttgccgt cggagattga agacgcaatg aggcgagaaa
      601 tgtggcccaa aaaccgtcga aatcgcgatt gaccgtagat ttttcaacgt ggccgtcctg
      661 gatcatcgta tagagaatcc ccttgctctt ctcatagacg ttgcgtggat tgaccagcgg
      721 cgagaaccgt cgcggcgccg ccgggatgga ggccagaacc gcggcctcga tcggggtgag
      781 ggcgcgggcg tctttatcga agtaaaagcg cgccgccgac tgtaccccgt agacgccatg
      841 cccgaagtac acctgattca aatacatctc aaggatccgg tccttggtga attttttttc
      901 tatctccccg gcgagcagca attcaagtat tttacgcctg atggttcgtt cgcgtccggt
      961 atacagctgc ttcacgagct gttgtgttat cgtggaccct ccctggcgga tttccccggc
     1021 ggcgatgtcg acgactagcg cacggagcac gctcaatgga tcgattcctc gatggcgata
     1081 aaaaccgcga tcttcggcgc ttaaaaatgc tgcccgcacc accgagggga tggcgcttaa
     1141 atcggcatag cttcgcttct cgtcatagag ttccgagatg agaacgccgt gggcatcgta
     1201 tagtttcgtc gaaagagcgg gattgaacgg cttgatggtg cgaatctcct catgtgagac
     1261 gaagtagacg gtaagaaaat acccaagcgg agcgaggagc agcagcccaa tcgtaattgc
     1321 gattgccgtt cgaataaccg tttttgtccg gtcagaattc gtcatgttcg cgcgccttcc
     1381 gctcgtgctg gtccgctttc agcgagtctc ccgtgaagcg atatacatcc gagaggagca
     1441 gatgggatgc cacgctcttc ggattgatcg caagcgccgc ctggaggcat ttcatggtgt
     1501 gccgtatatg ccgcgcgcgg aatgcccgat cgcgattatc gaacagcaga tgcgccatgt
     1561 agacgagcgc acgctcgttt tcgggatcga tcgataacaa gcggtcgata atcgccgccg
     1621 ccttggaaat cttttttgtc cgtcgatacg cttccgcggc cgcgaagtac gcatcggcaa
     1681 gcgtggggca tacctccatc gattgatgat acgcggcgag cgcgcgatcc attttcttcg
     1741 ctttttcgag ttcaatcccg aggttcatga tgcgagaacc ctcctcgcgg cacaacgcgc
     1801 tgcacgcatt gaacgacgcc cgtgcggatg tccgatcgcc cgcaccgacg taggagcgga
     1861 tgagattgaa ctgggccccc atattgttcc gattgatgtc gaccgcccgc cgataatggg
     1921 agcgcgattc atcgaacttt ccgatcgtat cgtacagcgc gccgatacgg acatgaatat
     1981 catcctgctc gggttttatc tcgagggagc gattgaagta gcgaatggcc tttttgatct
     2041 tgtggcgacg cgcataaata tcgccgatca tattcgccgc atccgtgagg gtgtcgttta
     2101 gggtaagcgc tcgcgtcagg ttctccagcg cgaatccgaa catctgttct cggtactgga
     2161 cggttccttt ttttaaatag tactcccaat cgttttgggc atgagctacg atgtgtgata
     2221 ataacaaaag tgagaaaatc gctgaatatt ttattgactt tttcacgggc accaatttaa
     2281 tgacttttcg tttcacagga ctaacggtca ggtagctcag tcggtagagc agtggactga
     2341 aaatccgcgt gtcggcggtt cgattccgtc cctgaccaca ttcactattc ttctaagcgc
     2401 cagttaacgc accacatcag taaacaaccg ccttccgcca atccgtctgg gcttccagtg
     2461 ctttccgtcg atgtcgtcgg caataacgcc tttcgagctg gcatgataga accgctcccg
     2521 tgaaaccgcc aaaccgacat gcgtgatctt cttgcggtcc ggtgacgccg agaagaacag
     2581 caggtcgcct gcgcgatacg ccgtggtcga cgacagcgcg gttccggcct gtatccgatg
     2641 ctgtcccaac gatgatcgcg gcaccaattt cggcggcaca ccgatcaaac gatcggtcaa
     2701 caccatccgg gtaaaccccg agcaatcgat tccgcttttc gaaaggcctc cccatttgta
     2761 cggaacgccg atccatggct tgatcgcgga ttgtagtgaa tcaatacgaa gccgtttcca
     2821 tcggtcgatt gccggctcct cgggtcggag cttttcgggg ggcgtttcct catcatgatg
     2881 atacggcgtg tcgggctcaa tcggaccgtc acgatgttca tcactgcgag aaagcgaacg
     2941 gatacgtgaa tcgatttttt gcgccgtagt gacgatgcgc tgcaggtctg atcgcgccct
     3001 cgattgcccc acatggatac gctgtatcgt taccaggttt tccaatattt cattgatgcg
     3061 ttcataggta actttcgaaa catcagcatg caaggcggtg aaaatgatac gggaaaaacc
     3121 gcccggtggc acttttgcgc cggcgcgggg aatcgagcgc aacagccgtt gggagacgtc
     3181 cccggtgttt gcggaaatca gatgcgcgca aatgagaccg cgacctccgg cgagaatcga
     3241 aagagcgtcg aaaccagcat cgagcgcgcg ggtcaggaat gcggcccgga cgtgttccgg
     3301 caccccggca cgtgtggttt cccgattata cagcgagtac aggatgaaat cgtcggtcgc
     3361 gggttttcgg cgtgataggg cgggaatcag atcctctgcg ctcgtaaact cgatccccgc
     3421 tgagatcgcg ccctcgaacg caacgatgaa ccgcgccgcc tcctcggcgt caagccgctc
     3481 catgaccgcc cacggaataa gctgcgtggt aatggccacg acgcggggat ctgcggcgcg
     3541 aaaaccgagg cgctgcttca catggttttt aatcagaatt cgctgctggg cattgtagtc
     3601 gctcttgagg acagaatcga tt
//
LOCUS       DAORHF010000331         3613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28507_length_3613_cov_4.788364, whole genome shotgun sequence.
ACCESSION   DAORHF010000331 DAORHF010000000
VERSION     DAORHF010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28507_length_3613_cov_4.788364"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PKJ16_17780"
     CDS             complement(<1..947)
                     /locus_tag="PKJ16_17780"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HNV48898.1"
                     /translation="MKDVAIVSTGNELVYGSVQDENAYYMSREFFRTDFQVVLHLTAG
                     DVPDDLELCIRQAVGRADVVIVTGGLGSTEDDYTFRVLQRIFDCGARVHEPAYERLKS
                     FLKFMKRRELENDVRMVTVPEGAEVFDNEYGLAPGYAIRHGEKIIIAMPGVPREMQLM
                     FDGKVLPFLLWEHQAGVRENFFLRVVLMRESEVNELIKKMDLPFDEMTWGVTAVPGMN
                     NISFVKRGAGRLDRWAIMSEARRVFKDRLLDPDSESLEMELVKLLTEASATIGVAESC
                     TGGLIAKRITDVPGSSAVFRGGVVAYGNEAKIEILASLP"
     gene            complement(964..2631)
                     /gene="argS"
                     /locus_tag="PKJ16_17785"
     CDS             complement(964..2631)
                     /gene="argS"
                     /locus_tag="PKJ16_17785"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HNV48899.1"
                     /translation="MNVKDEIEQLIRESLQSAIRDGKLPDAELPFVKVEYPKDERFGD
                     YATPIAMESARVLRKSPLEIGAILQEYVGKAPLVERVEVVKPGFINLFVSFDSLANNL
                     SRVLAEGDGYGRTTRATPFRVNIEFVSANPTGPLNIVSARAAALGDSIANLYEATGAT
                     VDREFYVNDYGNQVTLLGRSVLCRYRELAGEAVAFPEDGYHGEYVKDIARHVREQHAA
                     EVESLPDDEARIAYMAEKAVAYNVAGQREDLARFNVRFARWFSEKTLHESGAVERTSE
                     LLGARGVLREEEGKKLFVATAYGDEKDRVVVRDDGRPTYLLADIAYHRDKLERGYDRI
                     IDVWGPDHHGYIARLAGAMTALGAPAGRFSILIAQQVNLIMEGEIVKMSKRLGQFSTM
                     RELIDEIGVDVARYFFVMRSLESHLDFDLALAKRESSENPVFYLQYAHARICSIFREA
                     EKRGVAYTPGAVDREPLQNREAVALLKLIARFPEEIVDAATQCEPHRIANYLLRFAQQ
                     YHRFYTEHRVISDDPVTTNTYLALCDAVRIVMRNGLRLLGVTAPERM"
     gene            2770..>3613
                     /locus_tag="PKJ16_17790"
     CDS             2770..>3613
                     /locus_tag="PKJ16_17790"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HNV48900.1"
                     /translation="MSILNVLKKNFFGVFVAMLTFLVLTVVYLKTPLLDLAETGTVNF
                     RFYLRDPMQDTNQIQAGVTTYTPNPRAREDIVILGIDLNTIRSFTQENVFWPFPWNVH
                     AKFVSYVASGNPKAIFFDIMFVDHKPFEKELADAIRKAGNVYVDYAFEEEKKHTKVPD
                     LAERMEALARFRLPLDPKDASPTWPGEIEPPTPLIANAARGVGFANVKPDPDHVNRTM
                     PLLIKHEGHYYPNPDLLIVMDYFGIGAKDVEIRMGKHIRLKNLPAEKMAKPNGDHKAV
                     TDHGK"
BASE COUNT          710 a         1177 c         1077 g          649 t
ORIGIN      
        1 tatggcaacg aggccaggat ctcgatcttg gcctcgttgc cataagcgac aacgccgccg
       61 cgaaacaccg ccgaggatcc gggaacgtcc gtgatgcgct tggcgatcag cccgccggtg
      121 cacgactcgg cgacgccgat ggtggcgctc gcttcagtga ggagcttcac gagctccatc
      181 tccagcgact ccgagtccgg atcgagcagc cggtccttaa atacgcgccg cgcctccgac
      241 atgatggccc accggtcgag ccgccccgcc ccgcgcttca cgaaggagat attgttcatc
      301 cccgggacgg ccgtgacccc ccaggtcatc tcgtcgaaag gaaggtccat cttcttgatg
      361 agctcgttca cctcggactc gcgcatgagg acgacgcgca ggaagaagtt ttcgcgcacc
      421 cccgcctggt gctcccatag cagaaacgga aggacctttc cgtcgaacat gagctgcatc
      481 tcccggggca ctcccggcat ggcaatgatg atcttctccc cgtggcggat cgcatacccc
      541 ggcgcgagtc cgtactcatt gtcgaacacc tcggcgccct ccgggaccgt gaccatgcga
      601 acgtcgttct ccagctcccg gcgcttcatg aacttcagga aggacttgag ccgctcgtag
      661 gcgggctcgt gcacgcgcgc cccgcagtcg aagatccgct gcagcacgcg aaacgtgtaa
      721 tcgtcctcgg tggagcccag accgccggtc acgatcacca cgtcggcccg gccgaccgcc
      781 tggcggatgc acagctcaag atcgtccgga acgtcaccgg cggtgaggtg cagcaccacc
      841 tggaagtcgg tgcgaaagaa ttcccggctc atgtagtacg cgttctcatc ctgcacgctg
      901 ccgtagacca gctcgttgcc tgtcgatacg atggcgacat ccttcatagc cgtctcccgg
      961 gggtcacatc cgttcgggcg ccgtcacgcc gagcaggcga aggccgttgc gcatgacaat
     1021 gcgcacggcg tcgcaaagcg ccagataggt gttggtggtc accggatcat cggagatgac
     1081 gcggtgttcc gtgtaaaagc ggtgatactg ctgcgcaaag cgcagcaggt aattggcgat
     1141 acgatgcggc tcgcactggg tcgcggcgtc gacgatctcc tccggaaacc gcgcgatgag
     1201 cttcaggagc gccaccgcct cgcggttttg aagcggctcc cggtcgacgg cgccgggcgt
     1261 ataggcgacg ccgcgcttct ccgcctcgcg gaagatggag cagatgcgcg catgcgcgta
     1321 ttgcagatag aagaccgggt tctccgagct ctcgcgcttg gccagcgcca ggtcgaaatc
     1381 cagatggctc tccagcgagc gcatcacgaa aaagtagcgc gcaacgtcca ccccgatctc
     1441 gtcgatgagc tcgcgcatcg tggagaactg ccccaagcgc ttggacatct tcacgatctc
     1501 gccctccatg atcaggttca cctgctgggc gatgaggatg ctgaagcgcc ccgcgggcgc
     1561 gcccagcgcc gtcatggcgc cggcaaggcg cgcgatgtag ccgtggtggt cggggcccca
     1621 gacgtcgatg atccggtcgt agccgcgctc cagcttgtcc cgatgatatg cgatatccgc
     1681 gagcaaatag gtggggcggc cgtcgtcgcg caccaccacg cggtcctttt cgtcgccata
     1741 ggcggtcgcg acaaagagct tcttgccttc ctcctcgcgg agcacgcccc gcgcgccgag
     1801 gagctcggag gtccgctcca ccgcgccgga ctcatggagc gttttttcgc tgaaccagcg
     1861 cgcgaatcgc acattgaagc gtgcgagatc ctcgcgctgg ccggcgacat tgtacgcgac
     1921 ggccttctcg gccatgtagg cgatgcgcgc ctcgtcgtcc gggagcgact ccacctcggc
     1981 ggcgtgctgc tcgcgcacgt ggcgggcgat gtccttcacg tactcgccgt gatagccgtc
     2041 ctcggggaac gccacggcct cgccggcaag ctcgcgatag cgacacagga ccgaacggcc
     2101 caggagcgtc acctggttgc cgtaatcatt cacgtagaac tctcgatcca cggtcgcacc
     2161 ggtcgcctcg tagaggttgg cgatggagtc gcccagcgcg gcggcgcgcg cggaaacgat
     2221 gttcagggga ccggttgggt ttgcggatac gaactcgata ttgacgcgaa acggcgtcgc
     2281 gcgcgtcgtg cggccgtatc cgtccccctc ggcgaggacg cgtgacaggt tgttcgcaag
     2341 cgaatcgaac gacacgaaga ggttgatgaa tccgggcttg acgacctcca cgcgctcgac
     2401 cagcggcgcc ttccccacgt actcctgcag gatggcgccg atctcgagcg gcgacttgcg
     2461 caggacccgg gccgattcca tcgcaatggg cgtggcgtaa tcgccgaagc gctcgtcctt
     2521 gggatactcc accttgacga agggaagctc cgcgtcgggc agctttccgt cacggatcgc
     2581 gctttggagc gattcacgga tcagttgttc aatctcgtcc ttgacgttca cacgatacct
     2641 caaacattat catacatatt cccaaaaaat gtcggaattg aaaatatttt tgtcaaccat
     2701 taaataaaaa acttgataaa tagccattcg cgctgtagcg attgagcata cacacaccag
     2761 gagacgacca tgtcaatcct caatgtcctg aaaaagaact ttttcggcgt gtttgtcgcg
     2821 atgctcacct tcctcgttct taccgtcgta tatctcaaaa cgccgctgct ggacctggcc
     2881 gagaccggaa cggtcaactt ccgattctat ctccgcgatc ccatgcagga cacgaaccag
     2941 atacaggcgg gcgtgaccac ctacaccccc aatccccgcg cgcgcgagga catcgtcatc
     3001 ctggggatcg acctgaacac catccgcagc ttcacccagg agaatgtctt ctggccgttc
     3061 ccgtggaacg tgcacgcgaa attcgtttcg tatgtcgcgt cgggaaaccc gaaagcgatc
     3121 ttcttcgaca tcatgttcgt ggaccacaag ccgttcgaga aggagctggc cgacgccatc
     3181 cgcaaggccg gcaacgtcta cgttgactac gccttcgagg aggaaaagaa gcatacgaag
     3241 gtccccgacc ttgccgagcg catggaggcg ctcgcgcggt ttcgcctgcc gctcgatccg
     3301 aaggacgcct cccccacgtg gccgggtgag atcgagccgc ccaccccgct tatcgcgaat
     3361 gccgcccgcg gcgtgggctt cgcgaacgtg aagcccgacc ccgaccacgt gaatcgcacc
     3421 atgccgcttc tgatcaaaca cgagggccac tactatccca accccgacct gctgatcgtg
     3481 atggactact tcgggatcgg cgcgaaggac gtcgagatac ggatggggaa gcacatcagg
     3541 ctcaagaacc tgcccgcgga gaagatggcc aagccgaacg gggaccacaa ggcggtgacc
     3601 gaccacggca agt
//
LOCUS       DAORHF010000332         3611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28543_length_3611_cov_4.318616, whole genome shotgun sequence.
ACCESSION   DAORHF010000332 DAORHF010000000
VERSION     DAORHF010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28543_length_3611_cov_4.318616"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PKJ16_17795"
     CDS             complement(<1..722)
                     /locus_tag="PKJ16_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48901.1"
                     /translation="MTTEHGTDAPLFAGVVHKQLNPVVEFLARIFFRNFDIDPNSLTT
                     LKEYSEKGSVVFASFHGSNISLLILHHVLRRTGVSPPDYAMEYNPFLLQTLGYVFSRL
                     GHFLNGLFSRRDATITTEFDYVEGLVRSGKSILVSLMSRRFFLRRYLEIRHDSIIALV
                     EMQRRTQRPIFLIPEMIFWNMNPEKSRSILSSSALGDRRFLQALFAVVKSVTPSFVRL
                     STPINLQEEIASSPSADSAHIA"
     gene            complement(750..1079)
                     /locus_tag="PKJ16_17800"
     CDS             complement(750..1079)
                     /locus_tag="PKJ16_17800"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48902.1"
                     /translation="MVFPHEYKNKTLVVKIEERDDPLSEIFSDEFFGELIRLNVHGAR
                     AVAFDLTGKQYFNSADLGVLIKIKDMLMDEGLELILINTSDKTRELLQMVGLIDFFHL
                     VEREQDI"
     gene            1388..1461
                     /locus_tag="PKJ16_17805"
     tRNA            1388..1461
                     /locus_tag="PKJ16_17805"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1422..1424,aa:Pro,seq:cgg)
     gene            1593..2015
                     /locus_tag="PKJ16_17810"
     CDS             1593..2015
                     /locus_tag="PKJ16_17810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV48903.1"
                     /translation="MSESGKIEIFKATYPSKREIRKDIINALVNAISQNNIKIKINEN
                     ELYLIIDEALTNAMEHGNKWDPAKSVSLRMVKNGKHLHLEIKDQGPGFKPESNAIDAN
                     NIKNLKPRGRGIYIIKQFCSPRWSDNGNTVDLQIELVN"
     gene            2019..2747
                     /locus_tag="PKJ16_17815"
     CDS             2019..2747
                     /locus_tag="PKJ16_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48904.1"
                     /translation="MACRRNSTRVPLAPVSLLLSIAITLGIANLWRPAIDHAPDRNAH
                     AGIMDSIGETKNTVEGLGRFVGSIRTAVSAITGFFGFRAIVLFCAVLFVSMGLSQIGV
                     PPGLWSFCGAMAIVNAIWLAWMRSFSPDSSAYLSTMLSANAMVLAPVAALGVIRWIAP
                     RAFGFARRLLRSLPVFRGRPVSAERLTALLSDYQRASGDLSTQLTRDILAGGPDGVVL
                     SSTSKKTLDEINSIIERLKATHRN"
     gene            2806..3138
                     /locus_tag="PKJ16_17820"
     CDS             2806..3138
                     /locus_tag="PKJ16_17820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48905.1"
                     /translation="MDIRNTKSGKTVIAHLSGQFTYSDDKKFMDTARELVLDQPLTLA
                     IDVSGLPHVNSTLIAAFLAILKMANEQGCEFVIYGMNPGVMSVLEKVFSSDLVPLLTE
                     EEFMRKYL"
     gene            3161..>3611
                     /locus_tag="PKJ16_17825"
     CDS             3161..>3611
                     /locus_tag="PKJ16_17825"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HNV48906.1"
                     /translation="MSEKKIGIITAGGDCPGLNAVIRSVVKFAINDCGARAVGFLDGF
                     RGLVLNEYVDLTFDRVSNILSMGGTILGSSNRDNPFAFYYIDDQKEPMDVSDKVVETY
                     ERHDLSALICIGGDGTLSMSHEFSKKGLNIVGVPKTIDNDVQGTDQTF"
BASE COUNT          819 a         1045 c         1026 g          721 t
ORIGIN      
        1 atcgcgatat gcgccgagtc cgcggacggc gagctcgcga tctcctcctg taggttgatg
       61 ggcgttgaga gacgcacgaa cgacggggtc acgctcttga cgacggcgaa cagggcctgc
      121 aggaagcgac ggtcgcccag ggcgctcgag ctcaagatac tgcgcgactt ctccgggttc
      181 atgttccaga agatcatctc ggggatcagg aagatggggc gctgggtacg ccgctgcatc
      241 tcgacaagcg cgatgatcga atcgtgccgg atctcgagat agcgtcggag gaagaagcgg
      301 cgcgacatga gggagacgag gatgcttttc cccgagcgca caagcccctc gacatagtca
      361 aattccgtcg tgatggtcgc gtcccgacgc gaaaacaggc cgttgaggaa atgccccagg
      421 cgcgagaaga cgtaccccag ggtttgcaga aggaagggat tgtactccat ggcgtagtcc
      481 ggcggactca cgcccgtgcg ccgcagcaca tggtgcagga tgagcaggga aatattcgat
      541 ccatgaaatg aggcgaagac gacgcttccc ttctcgctgt actccttgag cgtcgtgagg
      601 ctgttggggt cgatatcgaa gttgcggaag aatatccgcg caagaaactc gacgaccgga
      661 ttgagctgct tatgtacgac cccggcgaag agcggggcgt ccgttccatg ttcagtggtc
      721 ataatcgcac gcaccgtccc gtacgggggt tagatgtcct gctcccgctc cacgaggtgg
      781 aagaaatcga tgaggcccac catttgcaac agctcccgcg tcttatccga ggtgttgata
      841 agtatgagct cgaggccctc gtccatgagc atgtccttta ttttgatgag cacccccaga
      901 tcggcactgt tgaagtactg cttcccggtg aggtcgaagg cgactgccct cgcaccatgc
      961 acgttgagcc gtatgagctc gccgaagaat tcgtccgaaa agatctccga caggggatca
     1021 tcacgctcct cgatcttgac gacgagggtt ttgtttttat actcgtgcgg aaaaaccaaa
     1081 ccaggccacc tcttcccgtg aatatgtcag aaacccgcaa cgtgcgtacg ttcatacaaa
     1141 cgaatgtgag aggcaagcaa tttttcaggt tattgtcgat ttctatgaat ctgtacaaaa
     1201 attagaccct gtggccttgt tactatgcat gtctagcccc tggaccgcgt gcagcacatg
     1261 tgataaattc aaaagtgtcc gggtgttgaa tttatcataa atcctccaaa aaaaaggatt
     1321 gataaatatc atcaataatg ttcattgcgt ttagtttccg atagtttcaa acgtcggcaa
     1381 ccgcatacgg gatgtggcgc agccaggtag cgcgcttcgt tcgggacgaa gaagccggga
     1441 gttcgaatct ccccatcccg aagcaaatgt ctttttcgac gctggttttc gactgagatc
     1501 agattgcgat acatataaac cgtgaatagt gcacatgtac gcaaccaatg gccggtacca
     1561 taatgcacat cggtggtagg ttataaagag gcatgtctga atcgggcaaa atagagattt
     1621 tcaaggccac ctatccgtcg aagcgcgaaa tccggaagga catcatcaac gccctcgtta
     1681 acgcaatcag ccagaacaac atcaagatta aaatcaacga aaacgagctc tatctcatca
     1741 tcgacgaagc gctcaccaat gcgatggagc acggcaacaa gtgggacccc gccaagagcg
     1801 tgagccttcg catggtgaaa aacggcaagc acctccatct cgagatcaag gaccaggggc
     1861 cggggttcaa gccggagagc aacgccatcg acgcgaacaa catcaagaat ctgaagcccc
     1921 gcggcagggg catatacatc atcaagcaat tttgcagtcc acggtggagc gacaacggaa
     1981 acaccgtcga cctccaaatc gagctcgtca actgattcgt ggcctgccgc cgcaacagca
     2041 cccgtgtccc acttgcgccc gtttccctgc ttctgagcat cgccatcacg ctcggcatcg
     2101 caaatctttg gcgtcccgcg attgatcacg cgcccgaccg gaacgcccat gcgggcatca
     2161 tggactccat cggcgagacg aaaaacaccg tcgagggcct gggccgcttt gtggggtcga
     2221 ttcgcacggc cgtgtccgcg atcacgggct ttttcgggtt ccgcgccatc gtgctgtttt
     2281 gcgccgtttt gttcgtctcg atgggcctct cgcagatcgg cgtcccgccc gggctgtggt
     2341 ctttctgcgg ggcgatggcg atcgtcaacg cgatctggct cgcctggatg aggagctttt
     2401 cgccggattc gagcgcctat ctctcgacca tgctctcggc aaacgccatg gtgctcgccc
     2461 ccgtggccgc actgggcgtc atccggtgga tcgcgccgcg cgcatttgga ttcgcccgta
     2521 ggctccttcg atcgcttccg gtatttcgcg ggcgccccgt gagcgccgag cggctcaccg
     2581 ccctcctctc cgactaccag cgggcgagcg gcgatctctc cacgcagctc acgcgcgaca
     2641 tcctcgccgg cgggcccgac ggggtcgttt tatcatcgac atccaagaaa acacttgacg
     2701 agatcaattc gatcatcgaa cgattaaagg cgacacacag gaattgatcg ccggcgcgcc
     2761 atggcggatc gaacatcgcc gcgccggcac ccagcggagt agagcatgga catacgcaac
     2821 accaagagcg gaaagaccgt cattgcccac ctgtccgggc agttcaccta ttcggacgac
     2881 aagaaattca tggataccgc gcgcgagctc gtgctcgacc agcccctgac gctcgcgatc
     2941 gacgtatcgg gcctccccca cgtcaactcg acgctgatcg ccgcattcct cgcgatcctg
     3001 aaaatggcga acgagcaggg gtgcgaattc gttatttacg gcatgaaccc gggcgtgatg
     3061 agcgtgctcg agaaggtgtt ttcctcggac ctggtcccgc tgctgaccga ggaagagttc
     3121 atgcggaaat acctgtagga ccgacaatac ggaggcacgc gtgagcgaga aaaagatcgg
     3181 catcatcacc gccggcgggg actgcccggg gcttaatgcg gtcattcggt ccgtcgtcaa
     3241 gttcgccatc aacgactgcg gcgcgcgcgc cgtgggcttt cttgacggct tccgcggcct
     3301 ggtgctcaac gaatacgtcg acctcacctt cgatcgtgtt tcgaacatcc tgagcatggg
     3361 cggaaccatc ctgggaagct cgaaccggga caacccgttc gctttctact atatcgacga
     3421 ccagaaagaa cccatggacg tgtccgacaa ggtcgtggaa acctacgaac ggcacgatct
     3481 gtcggcgctc atctgcatcg ggggcgacgg cacgctgagc atgtcgcatg agttttcgaa
     3541 gaaggggctc aatatcgtgg gcgtgcccaa gaccatcgac aacgacgtgc agggcaccga
     3601 ccagaccttc g
//
LOCUS       DAORHF010000333         3594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28766_length_3594_cov_4.308844, whole genome shotgun sequence.
ACCESSION   DAORHF010000333 DAORHF010000000
VERSION     DAORHF010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28766_length_3594_cov_4.308844"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..1803
                     /locus_tag="PKJ16_17830"
     CDS             145..1803
                     /locus_tag="PKJ16_17830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV48907.1"
                     /translation="MNTPHETIARIVETVAAVVDTARDVNRHSHERKDITQRSLAVLE
                     AVIGESGRLKSLYERVCDNLGGAGNANASNAEAFRVDVGYFKEIIRRFNDIKETFSTL
                     RREITKLSRLVSEIQNDTNEIFSLALNASIASSKYSHTSGVFDVLADKLNEMSIFINQ
                     NLEGIINVVAPIIDGIEKLVDNNNVMVVEMDRGHRNFLQVPDILDRQRVSIAALLDRA
                     QGSARKIDDQVVMMAELRDKIQRMDEDAGGAITGSANVIATGESLQKKVQEAGRMAET
                     GEPIDGAINHIREQATVISGNATAVNEKSKSQLDFSISAVQFCDSIVAESLELKEIAK
                     TFSAQSDESHRITETISDNLMQFNTQLGQIDAMLVSSNGTIQKFNSDFSQMDSIIGFL
                     KNIMKSMSVIGMLSRIESARDPEEFRGFMTIAENIRKLQEHIHANIPLIEHNIAQTHA
                     LIENVNVNFAEISRVFGTITQSSSQIIERLQDINRISAESQGISASIMDVSGGIDGSM
                     GSLRGQLTELSEVVKKPIEGSAANMERGKAIANLCDEVLSVPIG"
     gene            complement(1793..2764)
                     /locus_tag="PKJ16_17835"
     CDS             complement(1793..2764)
                     /locus_tag="PKJ16_17835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01212.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HNV48908.1"
                     /translation="MKKPDPSEDGTAPRPKTEWHGKPYNLFGDYLQHRYRTRVLKLPL
                     DAHLSCPNRDGTLGDDGCVFCRDGSASPGMSGIPSIAEQMRIAKGSFRRADGDTRFIS
                     YLQAYTNTYAPLQQLKDLYDRAIADPEVIGLMIGTRPDCCPDEVLDLIETYRRKDFEL
                     WIELGMQTTHDRSLRLLNRRHTFEHTVDAVDRITKRRIPVCLHCILGIPGETWEDMMR
                     TASTVASLPVQGVKLHHLHVIRGTRLEELHEHGRLPLLSQRDYVSTVCDFIERLPGDV
                     LIHRLMGDREESTLVAPTWGLHKGTVMRDIDDMFSRRGTHQGFLAIR"
     gene            complement(2742..>3594)
                     /locus_tag="PKJ16_17840"
     CDS             complement(2742..>3594)
                     /locus_tag="PKJ16_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48909.1"
                     /translation="ISGNSPAFTTMRAPITMMKLSRIAIHYLKNGNPQNAIYAIGLMQ
                     KSYGSSEFRNYLAPYVKVKNNIARASQLQSQALKLAKGPAKNRKIAIRRQAKARQLYR
                     SAMRHIGKRSLYAPLREHYAMEFKTAIGARTAVSVAAPPTSTAAPAEKMVSFSSVKRA
                     GLHTPIILTIENGDSLERSIEKALGPDRATLQKLVTSFSNAGEYRRVKVASYSKKKKK
                     MVWSWKRVRQPVSFEHGIYIVRLNRTLGVVSVSKTGRVPLVAGRYQNHQYLKNSYFAP
                     GEKTRSL"
BASE COUNT          785 a         1026 c         1092 g          691 t
ORIGIN      
        1 cactcacatc agggaaatcg aggcgatcgc cgccatgagc gacagcctca acgaaatgat
       61 caagaagatc gacgcgaaga cgaatcagat catcaaccag gtgatagagc tcgagaaggt
      121 tacctcgtag aaggcgacga caccatgaac acccctcatg agacaatagc gcgcatagtg
      181 gaaaccgtgg ccgcggtcgt tgacacggcg cgcgacgtaa accgccattc gcatgagcgc
      241 aaggacatca cccagcggag ccttgcggtg ctggaggccg tcatcggcga aagcggccgg
      301 ctcaaatcgc tctacgagcg ggtctgcgac aatcttggcg gtgcggggaa cgcgaacgcc
      361 agcaatgccg aggcgtttcg cgtcgatgtc gggtatttca aggagattat tcgtcgcttc
      421 aacgacatca aggagacctt ctcgaccctg cgtcgtgaga tcaccaaact ctcgcgactc
      481 gtcagcgaga tccaaaacga caccaacgaa atattctccc tcgcgctcaa tgcgtcgatc
      541 gcgtcgagca agtattcaca cacctccggc gtgttcgacg ttctcgcgga caagctcaat
      601 gagatgtcca tcttcatcaa ccagaacctc gagggcatca tcaacgtggt cgcgcccatc
      661 atcgacggta tcgagaaact cgtggacaac aataatgtca tggtggtgga gatggatcgc
      721 ggccaccgga acttcctgca ggtcccggac atcctggacc gtcagcgggt atcgatcgcc
      781 gcgctgctgg atcgggcgca ggggtcggcg cggaagatag acgatcaggt cgtcatgatg
      841 gccgagctga gggacaagat ccaacgaatg gacgaggacg ccggaggcgc catcaccgga
      901 tcggccaacg tgatcgcgac gggcgaatcg ctgcagaaaa aggtacagga agccggcaga
      961 atggccgaga cgggggagcc gattgacggc gccatcaacc acattcggga gcaggcgacc
     1021 gtcatcagcg gaaacgccac cgcggtgaac gagaaatcga agagccagct cgatttttcc
     1081 atcagcgcgg tgcagttctg cgattcgatc gtggcggaat cgctcgagct caaggagatc
     1141 gcgaaaacgt tcagcgcgca gtcggacgag agccaccgga tcaccgaaac catatctgac
     1201 aacctgatgc agttcaacac ccagctcggt cagatcgacg cgatgctggt gagctcgaac
     1261 ggcaccatcc agaaattcaa cagcgacttc tcgcaaatgg acagcatcat cgggttcctc
     1321 aagaacatca tgaagtcgat gagcgtcatc ggcatgctct cccgtatcga gtcggcccgc
     1381 gaccccgagg agtttcgcgg cttcatgacg atcgccgaga acatccgcaa gctccaggag
     1441 cacatccacg cgaacatccc gctcatcgag cacaatatcg cgcaaaccca tgcgctcatc
     1501 gagaacgtaa acgtgaactt cgccgagatc tcgcgcgtgt tcgggaccat cacgcagagc
     1561 tcgtcccaga tcatcgagcg cctgcaggac atcaaccgca tcagcgcgga gtcccagggc
     1621 atcagcgcct cgatcatgga tgtgtccggc ggcattgacg gctcgatggg atcgctgcgc
     1681 gggcagctca ccgagctctc cgaggtggtc aagaagccga tcgagggatc cgcggcaaac
     1741 atggagcgcg gaaaggcgat tgcgaacctg tgcgatgagg tgttgtcggt ccctatcgga
     1801 tagccaggaa cccctggtgc gttccgcgcc gtgagaacat gtcgtcgatg tcccgcatca
     1861 ccgttccctt gtgcaatccc cacgtcggtg cgacgagcgt gctctcctcc cgatccccca
     1921 ttagtcggtg gatgaggaca tctcccggca gacgttcgat aaaatcgcac accgtcgaaa
     1981 cataatcgcg ctgagaaaga agcggcagac ggccgtgttc gtgaagctcc tccagacgcg
     2041 ttccccggat aacatgcagg tggtgaagct tcaccccctg gacgggcagc gatgcgacgg
     2101 ttgacgccgt ccgcatcatg tcttcccatg tctcgccggg gatgcccagg atacaatgca
     2161 ggcacacggg tatcctccgc ttcgtgattc ggtcgacggc atcgaccgta tgttcaaagg
     2221 tgtggcggcg gtttaaaagg cgcaggctcc ggtcgtgcgt cgtctgcatt cccagctcga
     2281 tccagagctc gaaatccttt cgtcggtagg tctcgatgag atcgagcacc tcgtccgggc
     2341 agcagtcggg ccgcgtgccg atcatgaggc cgattacctc gggatcggcg atggcgcgat
     2401 catacaaatc tttgagctgc tgaagcgggg cgtaggtgtt cgtatacgcc tgcaggtagg
     2461 agatgaaacg cgtatcaccg tcggcgcgcc ggaaagagcc cttggcgatg cgcatctgct
     2521 cggcgatcga tggaatgccg ctcatgcccg gagaggcgga gccgtcgcgg cagaaaacac
     2581 aaccgtcatc gccgagggtc ccgtcgcggt tcgggcagct caggtgcgcg tcgaggggga
     2641 gcttgagcac gcgcgtacgg tagcggtgtt gaaggtaatc gccaaagaga ttatacggct
     2701 tgccgtgcca ttcggttttc ggacgggggg cggttccgtc ctcagaggga tcgggttttt
     2761 tcaccgggcg cgaaatacga atttttcagg tattggtggt tctggtaccg gccggcgaca
     2821 agcggcacgc gtccggtttt tgacaccgag acgacgccca gggtgcggtt gaggcggacg
     2881 atgtaaatgc catgctcgaa ggaaaccggc tgtcgcaccc gcttccatga ccacaccatc
     2941 ttctttttct tcttcgaata tgacgcgacc ttcacgcgcc ggtactctcc ggcgtttgaa
     3001 aacgatgtga cgagcttctg aagagtggcg cggtccggcc ccagggcctt ttcaatagac
     3061 cgttcgagag agtcgccgtt ttctattgta agtatgatcg gagtgtgcaa accggcgcgc
     3121 ttaaccgacg agaaggatac cattttctct gctggggccg ccgtcgatgt cggtggggcg
     3181 gcaacggaga ctgcggtgcg ggcgccaatc gcggttttga attccatcgc atagtgttcg
     3241 cgcagcggcg catacaggga gcgcttgccg atgtggcgca tggcggagcg atagagctga
     3301 cgggccttgg cctgccgtcg aattgcgatc ttccggtttt tcgccggtcc cttcgcaagt
     3361 ttcagtgcct gcgattggag ttgcgacgcc cgggcgatat tgttcttcac ttttacatag
     3421 ggtgcaagat agttccgaaa ctccgacgaa ccgtaggatt tctgcataag cccgatcgca
     3481 taaatcgcat tctgcggatt gccgtttttc aggtaatgga tcgcgattcg cgaaagcttc
     3541 atcatggtga tgggcgcgcg catcgtcgtg aaggccggac tgttcccgga gatg
//
LOCUS       DAORHF010000334         3580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28929_length_3580_cov_5.915177, whole genome shotgun sequence.
ACCESSION   DAORHF010000334 DAORHF010000000
VERSION     DAORHF010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28929_length_3580_cov_5.915177"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PKJ16_17845"
     CDS             complement(<1..256)
                     /locus_tag="PKJ16_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HNV48910.1"
                     /translation="MSAPLMKSVSGIRGIVGESLTPELVTSVAAAFAAYANGGTVVVG
                     RDSRCTGGAIAQCVSSVLALAGCDVVDIGIVPTPTVQVMVE"
     gene            complement(258..3008)
                     /gene="secA"
                     /locus_tag="PKJ16_17850"
     CDS             complement(258..3008)
                     /gene="secA"
                     /locus_tag="PKJ16_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HNV48911.1"
                     /translation="MVDKILSLFFGTKHERDIKRLTPLVQRINALEPDMKKLSLDEMR
                     SKTAEFRRRLERGETLDDLLPEAFALAREASLRTLGMRPFDVQLMGGVVLHEGKISEM
                     KTGEGKTLVATMPVYLNALSGKGAHVVTVNDYLARRDAEWMSPIYTGLGLTVGIIQHD
                     MDPYARQEAYACDITYGTNNEFGFDYLRDNMVEHKSLRVQRPLNYAIVDEVDSILIDE
                     ARTPLIISGSTDESTKKYYLVNRIIPSLIEKQDFEVNEKDRTALLTEAGVKHVERLLK
                     LENMYDPQNIELVHHVNQALKAHTLFKRDVDYIVKEGEVVIVDEFTGRLMPGRRYSDG
                     LHQALEAKENVTIARESQTLATITFQNFFRMYTKLAGMTGTADTEAMEFKKIYGLDVV
                     VVPTNEPMVRKDYPDRIYRTEREKFNAIVDEIETLSRKGQPVLVGTISIERSEKLARI
                     LKAKGVPHNVLNAKFHEREAAIVAEAGRPGAVTIATNMAGRGTDIVLGGRKTYLDELE
                     SHAAVHDAGMWGEFRNAVLGGDFDRSAAHAESMLGQDKHKAQEIARKGREWTQNNKTV
                     VSAGGLHILGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDDLMRVFGSDRISNV
                     MQRLGMEEGQEIESRMVSRAIENAQKRVEGRNFEIRKHLLEYDDVMNTQREYIYRERN
                     EILEGEDISAKILGYIAEVCREEIEFIVGNAEKDDNGVETVVAWLRSKFLLELDPGLF
                     GAQRLPADEIVRIVEERLVERYREKERELEPENMRTLERMISLQVIDSKWREHLLGMD
                     ELREGIWTVGYGERNPLVEYKIQGSQIFGVTMNSIKQDIIEFLFKVQVERIVEEQPRA
                     FRSIGQEFHPELEQFGAGGGIMDVVGGEDPRAAQRHLARHEEVETVGGVKRKKTRRNR
                     RR"
     gene            complement(3146..>3580)
                     /locus_tag="PKJ16_17855"
     CDS             complement(3146..>3580)
                     /locus_tag="PKJ16_17855"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HNV48912.1"
                     /translation="DCARERAGRGYRMAMTMDADSRLQIGDVARLVGMSQRTIRYYEE
                     IGLMNSVRRLEGGKRVYTDDDVRRLKLIKRLKVLGLTLADMHELEAIWSIHRSNDRVL
                     GRLQEILTGHLDRLDGRIADLQLLRNEIVEYEERIKSKLARS"
BASE COUNT          555 a         1218 c         1057 g          750 t
ORIGIN      
        1 gctccaccat cacctgtacc gtcggcgtgg gaacaatgcc gatgtccacc acgtcgcatc
       61 cggcgagcgc gagcacggag gatacgcact gtgcgatcgc gccgcccgtg cagcggctgt
      121 cgcgaccgac gacgaccgtg ccgccgttcg cataggccgc gaaggccgcg gcgacgctgg
      181 tgacgagctc cggggtgagc gactcgccaa cgatcccgcg gatccccgaa acgcttttca
      241 tcaagggcgc gctcatgtta ccgcctcctg ttccgcctgg tctttttccg cttcacgccg
      301 cccacggtct cgacctcctc gtgccgcgcc agatggcgct gcgccgcgcg cgggtcctcg
      361 ccgccaacca cgtccatgat cccgcccccg gcgccgaatt gctcgagctc gggatgaaac
      421 tcctgcccga tcgaccggaa ggcgcgcggc tgctcctcga cgatgcgctc cacctgcacc
      481 ttgaagagaa actcgatgat gtcctgcttg atcgaattca tcgtcacgcc aaagatctgc
      541 gagccctgga tcttgtattc caccagcgga ttgcgctcgc cgtatcccac tgtccagatc
      601 ccctcgcgga gctcgtccat tcccagcagg tgctcgcgcc atttcgagtc gatcacctgc
      661 agcgatatca tccgttcgag cgtgcgcatg ttctcgggct caagctcgcg ctccttttct
      721 cgatagcgct cgacaagccg ctcctcgacg atgcgtacga tctcgtccgc cggcaggcgt
      781 tgcgcgccaa agagcccggg atcgagctcc agcaggaact tcgaccggag ccaggccacg
      841 acggtctcta cgccgttgtc gtccttctcc gcgttcccga caataaactc gatctcctcg
      901 cggcacacct ccgcgatata tccgaggatc ttcgccgaga tgtcctcgcc ctcgaggatc
      961 tcgtttcgct cccggtagat atattcccgc tgcgtgttca tcacgtcgtc gtattccagc
     1021 aggtgtttgc ggatctcgaa gttgcgcccc tcgacccgct tctgcgcgtt ctcgatcgcc
     1081 cggctcacca tccggctctc gatctcctgg ccctcctcca tgcccagccg ctgcattaca
     1141 ttcgagatgc ggtcggagcc gaacacgcgc atgaggtcgt cctcgagcga gaggtagaag
     1201 cgggatgatc cgggatcgcc ctgccggccg gaccggccgc ggagctgatt gtcgatccgg
     1261 cgcgcctcgt gccgctccgt ccccaggatg tgcaggccgc cggccgacac caccgtttta
     1321 ttgttctgcg tccattcgcg ccccttgcgc gcgatctcct gcgccttgtg cttgtcctgc
     1381 cccagcatcg attcggcgtg cgcggcgctt cggtcgaaat cgccgcccag caccgcgttc
     1441 cgaaactcgc cccacatccc ggcgtcgtgc accgccgcgt gcgactcgag ctcgtcgagg
     1501 taggtcttgc gtccgcccag gacgatgtcg gtcccccggc cggccatgtt ggtggcgatg
     1561 gtgaccgcgc ccggccgccc cgcctccgcg acgatcgcgg cctcccgctc gtggaacttc
     1621 gcgttgagga cgttatgcgg gactcccttc gccttcagga tccgcgcgag cttctccgat
     1681 cgttcgatcg agatggtccc caccagcacc ggctgtccct tccggctcaa ggtctcgatc
     1741 tcgtccacga tcgcgttgaa cttctcccgc tcggtccggt agatccggtc ggggtagtcc
     1801 tttcggacca tcggttcgtt cgtggggacc accaccacgt cgaggccgta gatcttcttg
     1861 aactccatcg cctcggtgtc ggcggtcccg gtcatcccgg cgagctttgt gtacatgcgg
     1921 aagaagttct ggaaggtgat ggtcgccagc gtctggctct cgcgcgcgat ggtcacgttc
     1981 tccttcgcct cgagcgcttg gtggagcccg tcggaatagc gacgcccggg catgagtcgc
     2041 ccggtgaatt cgtcaacgat caccacctcg ccctccttca cgatgtaatc gacatcgcgc
     2101 ttgaagaggg tgtgcgcctt gagcgcctgg ttcacgtggt gaacgagctc gatgttctgc
     2161 gggtcgtaca tgttctcgag cttgagcaat cgctccacgt gcttcacgcc cgcctcggtc
     2221 aagagcgccg tccggtcctt ctcgttcacc tcgaagtcct gcttctcgat gaggcttgga
     2281 atgatgcggt tgaccagata gtatttcttc gtcgattcgt cggtggagcc cgagatgatc
     2341 agcggcgtcc gcgcctcgtc gatgaggatc gagtcaacct cgtcaacgat cgcgtaattc
     2401 aacgggcgct gcacccgcag gctcttgtgc tcgaccatgt tgtcgcgcag ataatcgaac
     2461 ccgaactcat tgttggtccc gtaggtgatg tcgcaggcgt acgcctcctg gcgcgcatag
     2521 gggtccatgt cgtgctggat gatgcccacc gtgagcccga ggccagtgta aatggggctc
     2581 atccactcgg cgtcgcgtcg cgcgagatag tcgttcaccg tcaccacgtg cgcccccttc
     2641 ccggagagcg cgttgaggta gacgggcatg gtggccacga gggttttccc ctcccccgtt
     2701 ttcatctcgg atatctttcc ctcgtgaagg accacgccgc ccatgagctg cacgtcgaag
     2761 gggcgcatcc ccagagtccg cagcgaggcc tcgcgcgcga gcgcgaacgc ctcgggaagc
     2821 agatcgtcga gcgtctcgcc ccgctccagg cgtcgccgga actcggcggt cttcgaccgc
     2881 atctcatcga gcgagagctt tttcatgtcg ggctccagcg cgttgatccg ctgcaccagc
     2941 ggcgtgagcc gtttgatgtc ccgttcgtgc tttgtcccga agaacaagct caggatcttg
     3001 tcgaccattg ctgtttcctt tgcgaaagag ggattgtccg tcgcggacgc gcatggtgcg
     3061 ccccgcggag aattgactca taggggagaa tgcggacttt tttttcaaga aaaataatgc
     3121 gggaggcccg gggcgcggga cccgctcacg aacgggcgag cttgctcttt attcgttctt
     3181 catattcgac gatctcgttt cggaggagct gcagatcggc gatccgaccg tcgaggcgat
     3241 cgaggtgccc cgtgaggatc tcctgcagac gcccgagcac gcggtcgttg gagcggtgga
     3301 tcgaccagat cgcctctagc tcgtgcatgt cggcaagcgt gagccccagc accttgagcc
     3361 gcttgatgag cttgaggcgc cgcacgtcgt cgtcggtgta tacgcgcttg ccgccctcga
     3421 gacggcgcac cgagttcatg aggccgatct cctcgtaata gcggatggtg cgctggctca
     3481 ttcccacgag ccgcgcgaca tcgccgatct ggagtcgact gtcagcgtcc attgtcatgg
     3541 ccattcgata gccccgcccc gcccgctccc gggcgcaatc
//
LOCUS       DAORHF010000335         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_28958_length_3577_cov_4.306360, whole genome shotgun sequence.
ACCESSION   DAORHF010000335 DAORHF010000000
VERSION     DAORHF010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28958_length_3577_cov_4.306360"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PKJ16_17860"
     CDS             <1..1079
                     /locus_tag="PKJ16_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48913.1"
                     /translation="GAINIYGQRVLVPEGTAVGLTESQSIAFIDTWVSTVRMRMRGRA
                     MDLSGRIEFFANGDVRSATIGSPVTLQNGPHRVTVDRKISFYDNGMIESCTLASDVTL
                     TINGSRVVFEAGRSIGIKEPASQLHFYRNGRVRSGDINGGTYTHRGVRIPLTTSGVIS
                     FYEGGAIKLFPVEGGTVLRAGGQDIRVKGYKLMLYENGGVLMAELQEGSDFLFRGKPV
                     HADSSVYQAFFDERQEVVAFSIMTPREVTYRGNRITLPPYYAAGYADYDKKEISFADI
                     CWDDLKLPDNATIVPEPGLKYSTTKKFVKADRLINAKRKNCHTNDLALLAEGVREIMF
                     SDDVTMVIDGKRQVCKAMRWVKLR"
     gene            1373..3484
                     /locus_tag="PKJ16_17865"
     CDS             1373..3484
                     /locus_tag="PKJ16_17865"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV48914.1"
                     /translation="MGYMPEVVKSLLRKFDRRMTIRNKIMIGGAGIAIASMISFGLAG
                     MLITGGTIRQMAENDLAHITDNLVSLCDTQYDINQNIVASNLNVADFFVKGRTSLKPD
                     TPVRFTVENQMTKESQQTALPLMTVDNAGVSNDFRLVDRITGMIGGTVTIFQVMKNPD
                     GLLRVSTSVKRADGTRAAGTYIPPSSPVYKAVMSGETYKGSAFVVNDWYITAYKPIYD
                     GNAIIGAIYVGVRQSELEILRKSVLALKIGKRGYAYILDNHDGSRGNVIIHPTREGKN
                     LLDAKDIAGNLYVKEMLEEKSGSKVYTLAEPGGGPASKRLVNFRSIDRLGWVAVAEAD
                     YDEVFAPLSMLRGTNILISILVLVIIVTASVAFSGSINRVMNRIKGLLTSLSSGDYSG
                     NIPQRDIERTDEFGDMATLFNVLVRNTRDLLEKIMRTTSVLTASINDLTVSSREVSTT
                     SNQQAAAVKEIVSTMEDSDALSRTVASRIDEVARIAGQTRTLVEKGFALIQDSLGKME
                     EIRHTNTETISGVKDLGAQIEGIWEVVNIINGIADQTKIIAFNAELEAASAGESGKNF
                     RIVAAEVRRLADSTVSSTAQIKGRINEIQRASDSLILASEKGTERIQEGADLSSKLKD
                     VFGDILSSAEISAGSSQQIVSSVNQQVSAFEQILITLKQISSGIDEFVATTRSTSGAA
                     ESLKDMGEELKTSLSRYKISE"
BASE COUNT          764 a         1033 c         1158 g          622 t
ORIGIN      
        1 acggcgcgat caacatttat ggccagaggg tcctcgtgcc ggagggaacc gctgtcggtt
       61 tgaccgagtc gcagtccatc gccttcatcg atacctgggt ctcgacggta cggatgcgga
      121 tgcggggccg ggcgatggat ctgtccggga gaatcgagtt ctttgcaaac ggcgatgtcc
      181 ggtcggccac tatcggttcg cccgtcacgc ttcagaacgg tccgcaccgg gtgacggtcg
      241 acaggaaaat atctttttac gacaacggga tgatcgaatc gtgcacgctg gcctccgacg
      301 tgacgctgac gatcaacggg agcagggtcg tattcgaggc gggccggtcg ataggcatca
      361 aggaaccggc aagccagctt cacttctacc ggaacggcag ggtgcgttcg ggcgatatca
      421 acggcggtac ctatacccat cgcggggtgc ggataccgct gacgacatcg ggcgtaatat
      481 cgttctacga aggcggcgcc atcaagctgt ttcccgtcga ggggggaacg gtcctgcgcg
      541 cgggcgggca ggacatacgc gtaaaggggt acaagctcat gttgtatgag aacggcggcg
      601 ttttaatggc cgagcttcag gagggatccg atttcctgtt tcgggggaag ccggtccatg
      661 cggattccag cgtctatcag gcattcttcg acgagcgaca ggaggtcgtc gcgttttcga
      721 tcatgacgcc gcgggaggtc acctaccggg ggaaccggat cacgctgcct ccctattacg
      781 ccgcgggata cgcggattac gacaaaaagg agatctcgtt cgccgacatc tgctgggacg
      841 acctgaagct gcccgacaat gcgacgatcg tgcccgagcc cggattgaaa tatagcacca
      901 caaagaaatt cgtgaaggcg gaccgtttga tcaatgcaaa gcggaagaac tgccatacca
      961 acgacctcgc gctcctcgcc gagggggtgc gggagatcat gttctccgac gacgtgacga
     1021 tggtcatcga cggcaagcgg caggtgtgta aggcgatgcg gtgggtgaag ttgcgataga
     1081 ggggtggcag agtgttctct tcgcctagga actccccgct ggcttcgata cgccgttggc
     1141 tactcagcca gcggggtcag gttccatggt aatccgttcc agcaatagcc cggtggccga
     1201 gtacccgaag gcgtatcgaa gccaccgttt ggcgcgcctg cagagtccgt ctgtcccgaa
     1261 aaagagtgat cgcttcccgc ccgaccgtgc cgataatcaa aagcagtagg acggatacct
     1321 ccgccggttt caggcggggg cgttcgtgaa atacggcagt ggaggggcgt ccatggggta
     1381 catgcctgag gttgtgaagt cgctattgcg gaagttcgac cgccgcatga cgatccggaa
     1441 caagatcatg atcggggggg cggggatcgc gatcgcgtcg atgattagtt tcgggctggc
     1501 gggtatgctg atcaccggcg gcaccatcag gcagatggcc gagaacgatc tggcgcacat
     1561 tacggacaat ctggtgagct tgtgcgacac ccagtacgac atcaaccaga acatcgtggc
     1621 cagtaatctc aacgtggccg atttcttcgt caagggtcgc acgagcctca agccggacac
     1681 cccggttcgc ttcacggtcg aaaaccagat gacgaaagaa tcccagcaaa cggccctgcc
     1741 gctgatgacg gtcgacaatg ccggcgtttc gaacgatttc aggctggtgg accgcatcac
     1801 cggcatgatt ggcggcacgg tcaccatatt ccaggtcatg aagaaccccg acggcctgtt
     1861 gcgggtgtcg accagcgtaa agcgcgccga cggcaccagg gcggcgggga cctacatccc
     1921 gccctcgtcc cccgtgtaca aggccgtcat gtccggtgag acgtacaagg gatcggcgtt
     1981 cgtggtgaac gattggtaca tcacggcgta caagccgatc tacgacggca acgcgatcat
     2041 cggggccata tacgtgggcg tgcgccagtc cgagcttgag attttgcgga agagcgttct
     2101 cgcgctgaaa atcggcaagc gcggttatgc atacatcctc gacaatcacg acggcagccg
     2161 agggaatgtc atcatccacc cgacccggga ggggaagaac ctgctcgatg cgaaggacat
     2221 cgccggcaac ctgtacgtca aggagatgct cgaggaaaag agcggcagca aggtgtacac
     2281 cctcgcggag ccgggcggcg gaccggcatc aaagcggctc gtgaacttca ggtccatcga
     2341 ccggctcgga tgggtggccg tggcggaggc cgattacgac gaggtgttcg cccccctgag
     2401 catgctgcgg ggcaccaata tccttatcag cattctcgtg ctcgtgatca tcgtgacggc
     2461 gagcgtcgcg ttctccggct cgatcaaccg cgtgatgaac aggatcaagg ggctgctcac
     2521 cagcctctcc tccggggact attcgggcaa catcccccag cgggacatcg agcgaacgga
     2581 cgagttcggc gatatggcca cgctcttcaa cgtcctggtg cgcaataccc gcgatctgct
     2641 cgagaagatc atgcgcacca cgagcgtgtt gaccgcctcc atcaacgacc tcacggtgtc
     2701 ctcccgcgag gtgtcgacca cctcgaacca gcaggccgcg gcggtcaagg agatcgtctc
     2761 gacgatggag gactcggacg ccctgtcgcg cacggtggcc tcgcgcatcg acgaggtcgc
     2821 gcggatcgcg ggccagacgc gcacgctggt cgagaagggc ttcgcgctga tccaggacag
     2881 cctgggcaag atggaggaga tccgccacac caacacggaa accatcagcg gcgtcaagga
     2941 cctcggcgcg cagatagagg gcatctggga ggtggtcaat atcatcaacg gtatcgccga
     3001 ccagaccaag atcatcgcat tcaacgcaga gctcgaggcc gcatcggcgg gtgaatcggg
     3061 gaagaacttc cgtatcgtgg ccgccgaggt gcggcggctg gcggacagca ccgtatcgtc
     3121 caccgcgcag attaagggca ggatcaacga gatccagcgc gcctccgaca gcctgatcct
     3181 cgcctccgag aagggcaccg aacggatcca ggagggggcc gatctctcgt cgaagctgaa
     3241 ggacgtgttc ggcgatatcc tcagttcggc agagatatcc gccgggtcgt cgcagcagat
     3301 cgtgtcctcg gtcaaccagc aggtctcggc gttcgagcag atcctcatca cgctcaaaca
     3361 gatctcgagc gggatcgacg agttcgtcgc cacgacgcgg tcgacctcgg gggcggccga
     3421 gtcgctcaag gacatggggg aagagctcaa aacgagcctg tcccgctaca agatctcgga
     3481 gtaacagcgc catggaaaac agcgaactgc tgagcgcgat atccgcgacg gggcagggcg
     3541 acgcggccgc ggaagagccg gtccggctca ggaaggt
//
LOCUS       DAORHF010000336         3574 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29003_length_3574_cov_7.370276, whole genome shotgun sequence.
ACCESSION   DAORHF010000336 DAORHF010000000
VERSION     DAORHF010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29003_length_3574_cov_7.370276"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..868
                     /locus_tag="PKJ16_17870"
     CDS             245..868
                     /locus_tag="PKJ16_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48915.1"
                     /translation="MRAKWIRVIAIILAFGVAGCGASRTVKKNSVKSIRNVAIVSVYC
                     PAAITIDNQRQAVGFHAVDAAVQAKQRTADAMAEFRPRGLFLLNRAHAETVGQFERAF
                     GWKVQPLTASIKSGHYAALKQWSDERKDTSFHKMMGVDGTAFLWVVKDDDSLRALLGE
                     CCAKAGYDAVMIASFDFAYSKSWFGKSESPPRPAPSSACRSSIATVT"
     gene            871..1047
                     /locus_tag="PKJ16_17875"
     CDS             871..1047
                     /locus_tag="PKJ16_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48916.1"
                     /translation="MATDRADEREREYSVMADDTMPLKGWTADINDTTIKTFDSAKKK
                     SVANMVSLLKSELN"
     gene            complement(1077..2180)
                     /locus_tag="PKJ16_17880"
     CDS             complement(1077..2180)
                     /locus_tag="PKJ16_17880"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HNV48917.1"
                     /translation="MMLSGKGWSERARAGLERLIESGAGKGHAAVFDFDNTIVCGDIG
                     EATLALLAKERLIKKDDIPAELAPPFRSPAGDTVSLSACPDLTVYYEELLGATAHQAG
                     DFAPLSNGYVWAVEAMRGLSVAEVTGATERVNARSEPFVTKRIEVTPGVSSYPVPFFY
                     PEMIELIAALITNRYEVWIVSASNAWTIRWMALRALNPLLAALGCDEGIAPERVIGVS
                     TLLSDRQERLYKDHLLVRAERRYADMDREYLDGLRLTARTVYPAPTYSGKVACVLDLI
                     GRRPFLAAGDSPGDLPMLEYAENRLWVARLEKPSYQHEMAAIAEKSGGNWIAQKTLCA
                     NATGFACDTACTVETGPTKDAITASIAAIGRFI"
     gene            complement(2182..3408)
                     /locus_tag="PKJ16_17885"
     CDS             complement(2182..3408)
                     /locus_tag="PKJ16_17885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV48918.1"
                     /translation="MKNPDSRPAGAADLRAWQLRIFWLLWTAYASYYLCRVNFAVAQP
                     LILREFSDWTAAQIGLIPSIYAMCYAVGQVVNGTLGERFGARLLMTLALIGAATTNIL
                     FSMTSSFSMMLVLWAINGYAQSAGWSLLIQVISNWNTSARRGTLVGLISTCYQVGNVV
                     SWLLAGYLCDSIGWRAAFMVPGLVLVHMAVIFAVGLRNRPEDAGFPPVRDDIDHRPGP
                     AGSFPQAVLSTRDILVMTLQNRMIWILGMGFFCMNSVRYTFMNWTVQYMADFQDQSIK
                     GSAFTAIAIPLIGTLGAVAAGWASDHVFGRRRAPVCAIMLFGLAGFLIYGPDMLMSGA
                     ATIDLSHPRAASIATGLTMSLGATGAIFSGTGIGLLKDFAKGDWSLVFWVLAGLSVVS
                     ALLMVSIWNARPRGAK"
BASE COUNT          690 a         1070 c         1198 g          616 t
ORIGIN      
        1 agcgcatcgc cgagagcgcc cggcaggtct attatctgct gaagccccac ctcacgcccg
       61 aggcgcttgt cgaggtggag aagctcctcg actgacgcgc tcggcccgct ttttttcctt
      121 gcgtgccgcg ccgtacgccg atatacttca cctgccttca atcgggacag aaataaccga
      181 tcgacccgtc tgccgcagtg ccctgtgccc gcttgaaaaa aacataccgg aggagttcgg
      241 aaatatgcgt gcgaaatgga ttcgtgtaat tgcgatcatc ctggcgttcg gcgtcgccgg
      301 ctgcggcgcc tccagaacgg tgaagaagaa ttcggtgaag tcgatacgga acgtcgcgat
      361 cgtcagcgtc tactgcccgg ccgccataac gatcgacaac cagcggcagg ccgtgggctt
      421 tcacgcggtc gacgcggcgg tgcaggcgaa acagaggacg gccgacgcca tggcggagtt
      481 caggccgcgc gggctgtttc tgctcaacag ggcccatgcc gagacggtgg gacagttcga
      541 gcgtgccttc ggctggaagg tccagccgct caccgcgtcc ataaagtccg ggcattacgc
      601 cgcgttgaag caatggtcgg acgaacggaa ggacacgtct ttccataaga tgatgggagt
      661 cgacggtacg gccttcctgt gggtggtcaa ggacgacgat tcgctgcggg cgctgctcgg
      721 ggaatgctgc gcgaaggcgg gctacgacgc ggtgatgata gcgagcttcg atttcgccta
      781 cagcaagagc tggttcggca agtcggaatc tccgccaagg cccgcacctt cgtcggcgtg
      841 cagatcgtcg atcgcaacgg tgacttgatc gtggccaccg atcgggccga cgaaagagaa
      901 cgcgaatact ccgtgatggc cgacgacacc atgccgctca agggctggac ggccgacata
      961 aacgatacga cgatcaagac cttcgattcc gcgaagaaaa agagcgtcgc gaacatggtt
     1021 tctttgctga agagcgagct gaactagcga accgtccgga tcggttccgc aggcgtctaa
     1081 atgaatcgcc ctatcgccgc gatcgaggcg gtgatggcgt ccttcgtggg gccggtttcg
     1141 acggtgcacg cggtgtcgca cgcaaaacca gttgcgttcg cgcataacgt cttctgtgcg
     1201 atccagttcc cgccgctctt ttcggcgatc gcggccatct cgtgctggta ggagggcttt
     1261 tcgagccgcg cgacccacag gcggttttcc gcgtattcca gcatggggag gtcgcccggg
     1321 ctgtccccgg cggcgagaaa gggccgcctg ccgatgaggt cgagtacgca ggccaccttg
     1381 ccggagtagg tcggcgccgg gtagacggtg cgcgcggtga ggcgaagccc gtcgaggtat
     1441 tcccggtcca tgtccgcgta gcgccgttcc gcgcgcacca ggaggtgatc tttatagagc
     1501 ctttcctggc gatcggaaag gagcgtcgag acaccgatca cgcgctccgg cgctatgccc
     1561 tcgtcgcagc cgagcgcggc gagcaggggg ttcagcgcgc gaagcgccat ccagcgtatc
     1621 gtccaggcgt tgctcgccga gacgatccac acctcgtagc ggttggtgat gagcgcggca
     1681 atgagctcga tcatctccgg gtagaagaag gggacgggat aggagctcac gcccggcgtg
     1741 acctcgatgc gtttcgtgac gaagggttcg ctccgcgcat tcacccgctc cgtggcgccg
     1801 gtcacctcgg cgacggagag gccccgcatc gcctccacgg cccacacgta gccgttcgag
     1861 agcggcgcga agtcgccggc ctgatgcgcc gtggcgccca gaagctcttc ataatatacg
     1921 gtgaggtccg ggcaggcgga gaggctgacc gtgtcccccg ccggggaacg gaacggaggc
     1981 gcgagctcgg cggggatatc atcctttttt atcagcctct ccttcgccag aagcgcgagc
     2041 gtggcctcgc cgatgtcgcc gcagacgatg gtgttgtcga aatcgaagac cgccgcgtgc
     2101 ccctttcccg cgccggactc gatgagccgc tcgagccccg cgcgcgcccg ctccgaccag
     2161 ccttttcccg aaagcatcat attatttcgc cccccggggc cgcgcgttcc agatcgagac
     2221 catgagcagg gccgacacca ccgagagccc cgcgagcacc cagaacacga gcgaccagtc
     2281 gcccttcgcg aagtctttga gaaggccgat gcccgtgccg gagaagatgg cgcccgtggc
     2341 ccccaggctc atcgtgaggc ccgtggcgat ggaggcggcg cgcgggtggc tgaggtcgat
     2401 cgttgcggcg ccgctcatga gcatgtccgg accgtagata aggaagccgg ccaggccgaa
     2461 gagcatgatg gcgcagaccg gagcccgccg ccggccgaat acgtggtccg aggcccagcc
     2521 cgccgcgacg gcgccgagcg tgccgatgag cggtatggcg atggcggtaa aggcgctccc
     2581 ctttatgctc tggtcctgga agtcggccat gtactgcacg gtccagttca tgaaggtgta
     2641 gcgcaccgag ttcatgcaga agaagcccat gccgagtatc cagatcatcc tgttctggag
     2701 ggtcataacg agaatgtcgc gggtggagag caccgcttgc gggaaggatc ccgccgggcc
     2761 cgggcgatga tcgatatcgt cccgtaccgg tgggaagccc gcgtcctcgg gccggttgcg
     2821 gaggcccacg gcgaagatga cggccatgtg tacgaggacg agtcccggca ccatgaaggc
     2881 ggcgcgccag ccgatcgaat cgcagagata tccggccaga agccacgaga ccacattgcc
     2941 gacctggtaa caggtcgaga tgagcccgac gagcgtgccg cggcgcgcgg aggtgttcca
     3001 gttggagatg acctggatga gaagcgacca tccggccgac tgggcgtagc cgtttatggc
     3061 ccagagaacc agcatcatgg aaaaagacga agtcatcgag aagaggatgt tcgtcgtcgc
     3121 ggcgccgatc agggccagcg tcatgaggag cctggccccg aagcgctcgc cgagggtgcc
     3181 gttgacaacc tgtccgacgg cgtagcacat ggcatatatg gaaggaatga gtccgatctg
     3241 cgcggccgtc cagtcgctga attcgcgcag tatgaggggc tgggcgacgg cgaaattcac
     3301 acggcacagg tagtacgagg cgtaggcggt ccacaggagc cagaatatcc tgagctgcca
     3361 cgcccgcagg tcggccgcgc cggcgggccg ggaatcggga tttttcatgg tgccattctt
     3421 acgggagtaa catgagcgtg gcgagtttct tgcccatgaa accggtcgag tcaagagaaa
     3481 gggcgcccgg ctggtgtgaa attattatga atattttccg gaaaacgcgc tgtttttatg
     3541 ccgggcggca ggcggggtgt cgaatcttgc taag
//
LOCUS       DAORHF010000337         3556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29233_length_3556_cov_4.297058, whole genome shotgun sequence.
ACCESSION   DAORHF010000337 DAORHF010000000
VERSION     DAORHF010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29233_length_3556_cov_4.297058"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKJ16_17890"
     CDS             complement(<1..298)
                     /locus_tag="PKJ16_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012662619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HNV48919.1"
                     /translation="MARRITGIISALIIAGTAFAAIGCSRDTGNKIVIKGSTTVLPIT
                     QKVAEAYRKTNKVAISVEGSGSGNGIKAVIDGTCDIGNSSREMKGNEIEEAKKKG"
     gene            524..1276
                     /gene="pstB"
                     /locus_tag="PKJ16_17895"
     CDS             524..1276
                     /gene="pstB"
                     /locus_tag="PKJ16_17895"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HNV48920.1"
                     /translation="MDKITSVNLTFSYGGVPAIKNINLTVEPNTVMALIGPSGCGKST
                     FLRCINRMNDIIPNIGIDGDILIDNESIYHRDFDVTGLRRRVGMVFQKSNPFPKSIFE
                     NIAYGLRINGIKNTYEIERTVEQSLKRTALWDEVKDRLDQSALGLSGGQQQRLCIART
                     IAVAPEIILMDEPASALDPLSTQKIEELIQDLKQNYTIIIVTHNMQQAARVSDHTAFF
                     YLGELIEVNRTDMIFTKPDNEMTENYITGKFG"
     gene            1286..1960
                     /gene="phoU"
                     /locus_tag="PKJ16_17900"
     CDS             1286..1960
                     /gene="phoU"
                     /locus_tag="PKJ16_17900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HNV48921.1"
                     /translation="MITHLEQELEAVKTRIFEMSDLAMEAIARSVESLKKGDVRLAER
                     VIQGDSELDRLEIAIDEECVRVLVTKQPAAKDLRLVTAFLKINTDLERIGDLATVVAK
                     ETIRMAGKPPVKPLVDIPRMAVLCAEMIKESFLAIAERDAARARGVIGRDREIDELNM
                     QIYRELFSYMAENPHSISPALSLIMASKALERIGDHATNIAERAIYYIEGVDVRHGAH
                     EGSEGE"
     gene            2012..2707
                     /locus_tag="PKJ16_17905"
     CDS             2012..2707
                     /locus_tag="PKJ16_17905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HNV48922.1"
                     /translation="MKSAKKNIFVVDDEADILEILKINLAEAGFIVHTFSSGETAMSA
                     LKKIIPDLIILDVMMSGMDGYDFCKTVRGIPDFHAVPIIFLSAKSEEFDKVLGLELGG
                     DDFITKPFGIKELISRIKAVLRRAEKQLRAAEHEALRFKGVELHPDKYIVTVDGEEAK
                     LTKTEFEILHLFMRYRGKIFTRDNIIDSIRGNDVYVVDRTIDVHVMNLRKKLGPYGES
                     IKTFSGVGYGIKE"
     gene            2752..>3556
                     /locus_tag="PKJ16_17910"
     CDS             2752..>3556
                     /locus_tag="PKJ16_17910"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV48923.1"
                     /translation="MFNTIGNKIIVSVAVLIAALIGALSLYVDARARDNHLHMVRRVM
                     AEKADLLGLFLSDGRLNRSALSRADAALMTDIARIGDMRITVIARDGRVLADTEVADL
                     STLDNHRYRAEVMGALETGSGESVRYSDTLKANIMYYAELHHDLVVRVAKPLADAEAS
                     LARLRRAIVVAGLSAILLTVAGAFVLVRRVTRPVRETIAFAERFSAGDFSHRILNYND
                     DEIGTLQKALNRMADTIIEKMNGLILEQKKLQVTIESITDGIAVIDHSKR"
BASE COUNT          733 a         1107 c         1073 g          643 t
ORIGIN      
        1 cgcccttctt cttcgcctcc tcgatctcgt tgcccttcat ttcgcgcgac gagtttccga
       61 tgtcgcaggt gccgtcgatg accgccttga tcccgttgcc cgagccgctg ccctcgaccg
      121 agatggccac cttgttcgtc ttccggtagg cctcggccac cttctgggtg atggggagca
      181 cggtggtgga tcccttgatg acgatcttgt tgccggtgtc gcgcgaacag ccgatcgcgg
      241 cgaacgccgt accggcgata atgagcgccg atataattcc ggtaattctt cttgccattg
      301 ttgcctcctt gggtgctgta tgatgtaccg ataccgggac cgtgttaaga ttttattaag
      361 aaacgcttta aaaaccgtga agccatgaag aaatccgaaa ttaacaaaat ctttacaaaa
      421 ccatcacgga atatttaaca ccgggtttta ttgtgttttc aattgattac gtgtattgta
      481 gtaagggcaa ggcgctgcct tgaccctact accgggaaaa cgcatggata aaataacatc
      541 ggtaaacctc accttctcgt atggcggcgt tccggccatc aagaacatca atctcacggt
      601 cgagcccaat accgtgatgg cgctcatcgg gccgtcgggc tgcgggaagt ccaccttcct
      661 gcggtgcatc aaccgcatga acgacatcat cccgaacatc ggcatcgacg gcgacatcct
      721 catcgacaac gaatcgatct accaccgcga tttcgacgtg acgggcctgc ggcgccgggt
      781 ggggatggtg ttccagaagt cgaacccgtt tcccaagtcc atcttcgaga acatcgccta
      841 cgggcttcgc atcaacggca tcaagaacac gtacgagatc gagcggacgg tggagcagtc
      901 gctcaagcgc acggccctct gggacgaggt gaaggaccgc ttggaccagt cggcgctggg
      961 gctctccggc ggccagcagc agcggctctg catcgcgcgc accatcgcgg tcgcgcccga
     1021 gatcatcctc atggacgagc ccgcctcggc gctcgatccc ctctccacgc agaagatcga
     1081 ggagctcatc caggacctca agcagaacta caccatcatt atcgtgaccc acaacatgca
     1141 gcaggcggcg cgcgtgagcg accataccgc cttcttctat ctgggcgagc tcatcgaggt
     1201 gaaccgaacc gacatgatct tcaccaagcc cgacaatgag atgacggaaa actatatcac
     1261 cggaaaattc ggctaaggag cgcgcatgat tacccatctg gaacaggaac tggaggcggt
     1321 caagacccgc atcttcgaga tgtcggacct cgcgatggag gcgatcgccc gctcggtgga
     1381 atcgctcaag aagggcgacg tgcgcctggc cgagcgcgtg atacagggcg actccgaact
     1441 cgaccggctc gagatcgcca tcgacgagga gtgcgtccgc gtcctcgtga ccaagcagcc
     1501 cgccgcgaag gacctgcggc tggtgacggc ctttctcaag atcaacaccg atctggagcg
     1561 catcggcgac ctggcgacgg tggtcgccaa ggagaccatc cgcatggccg gcaagccgcc
     1621 ggtgaaaccg ctggtggaca tcccgcgcat ggccgtcctg tgcgcggaga tgatcaagga
     1681 gtcgttcctg gccatcgccg agcgcgacgc cgcgcgggcg cgcggcgtga tcggccgcga
     1741 ccgcgagatc gacgagctca atatgcagat ctatcgcgag ctcttctcgt acatggcgga
     1801 gaacccgcac agcatctcac cggcgctgtc gctcatcatg gcgtcgaagg cgctcgagcg
     1861 catcggggac catgcgacca atatcgccga gcgcgccatt tactacatcg agggcgtgga
     1921 cgtgcggcac ggcgcccacg aggggtccga gggcgagtag cccggaacgc gcggacgcat
     1981 tcgcgcgtgc gcagatgacg gaaggggcca gatgaaatcc gcaaagaaaa acatcttcgt
     2041 cgtggacgac gaggccgaca tcctggagat cctgaagatc aacctcgccg aggcggggtt
     2101 catcgtgcat accttctcgt cgggtgagac ggcgatgagc gcgctcaaga agatcatccc
     2161 cgacctcatt atcctggacg tgatgatgag cggcatggac ggctacgatt tctgcaagac
     2221 ggtccggggg atcccggact ttcacgccgt gcccatcatc ttcctttccg cgaagtccga
     2281 ggagttcgac aaggtgctgg ggctcgagct cgggggcgac gatttcatca ccaagccctt
     2341 cgggatcaag gagctcatct cgcgcatcaa ggcggtgttg cgccgggcgg agaagcagct
     2401 gcgcgccgcc gagcacgagg cgctgcgctt caagggcgtg gagctccatc ccgacaagta
     2461 catcgtcacg gtggacggcg aggaggccaa gctcaccaag accgagttcg agattctgca
     2521 cctgttcatg aggtaccgcg ggaagatctt cacgcgcgat aatatcattg acagcatccg
     2581 cggaaacgac gtatacgtgg tggaccgcac catcgacgtg cacgtgatga accttcgcaa
     2641 gaagctcggg ccctacggcg agtccatcaa gaccttttcc ggcgtcgggt acggcatcaa
     2701 ggaatagccc gcgccgttcg gcggcccgtc cgtccgggag ggagcgcgat catgttcaac
     2761 accatcggca acaagatcat cgtctccgtg gccgtgctga ttgctgcgct gatcggcgcg
     2821 ctgtcgctgt atgtcgacgc ccgcgctcgc gacaatcacc tgcacatggt ccgacgcgtc
     2881 atggccgaga aggcggacct cctcgggctc tttctgtccg acgggcggct caatcgctcg
     2941 gccttgtccc gcgccgacgc cgcgcttatg accgatatcg cgcggatcgg ggacatgcgg
     3001 atcaccgtga tcgcgcgcga cggcagggtg ctcgccgaca ccgaggtggc ggacctctcg
     3061 acgctggaca atcaccgcta tcgcgccgag gtgatggggg cgctcgaaac ggggtccggc
     3121 gagagcgtcc ggtacagcga caccctgaag gcgaacatca tgtactatgc cgaactccac
     3181 cacgatctcg tggtgcgggt cgcgaagccc ctggccgatg ccgaggcgag cctggcgcgc
     3241 ctgcgccgcg caatagtggt tgcagggctc tcggcgatcc ttctcaccgt ggcgggggcc
     3301 ttcgtcctag tccggcgcgt cacgcgcccg gtccgcgaga ccatcgcctt cgccgaacgc
     3361 ttttccgcag gcgacttctc gcaccgcatc ctcaactaca acgacgacga gatcggcacg
     3421 ctgcaaaagg ccctcaatcg catggccgac accatcatcg agaagatgaa cgggctcatc
     3481 ctggagcaga agaagctcca ggtgaccatc gagagcatca ccgacgggat cgcggtgatc
     3541 gaccattcga agcggg
//
LOCUS       DAORHF010000338         3554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29264_length_3554_cov_5.741640, whole genome shotgun sequence.
ACCESSION   DAORHF010000338 DAORHF010000000
VERSION     DAORHF010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29264_length_3554_cov_5.741640"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..1932
                     /locus_tag="PKJ16_17915"
     CDS             313..1932
                     /locus_tag="PKJ16_17915"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV48924.1"
                     /translation="MTQKKGKQLNRNLAATRHDEIFTKIITKNHKRLFISCIPIYLLA
                     NVAAVGLLITGRASKHLTWEAIILEFSIAIPILALTYLIVKRMKGRPSSAYVAIAGAT
                     MFFFIFQYFIFGTPELFATHYIVLIFSVLYFNERVALFAFLMVVLSQTGLFALRPELR
                     PDLVPGAHTSALVVRMLIYLFVGIGATVGSKASRELLMLAIEKADESEKNLTGLRQVV
                     GAVAKSVKVLKSQVESQDNVVTEINDLSQKQSASLEEVSSTLEELSGNSESITKTAKS
                     LFEEMGIANDSVEDLRKVYDVILNSASSINTSISEVTKYSKQTFEQIKIIMEKFQILG
                     DKGTDISNFIQVINDIADKVNLLSLNASIEAARAGEYGRGFSVVAEEISKLADATSKN
                     ASEIEKLINENKLLLEGSREFVEEFSRMVIQLNNAIGSITKEIGSVGDLIVDIGNTVK
                     IINSMGTKIYDSSKNIETSTNEQQVATEESSRSVQYVYEASEQLVAVATNITYSTKTI
                     NRLADDLIALVQTIHFEEAAASESAETGSAA"
     gene            2071..3387
                     /locus_tag="PKJ16_17920"
     CDS             2071..3387
                     /locus_tag="PKJ16_17920"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV48925.1"
                     /translation="MSYSLINYCLAPVLTVIAVWIDLSSMHWSHVVVLGGVGSAVTLG
                     CMGLVMARTHISNRFSLMLMLGQYIVFLAIFAVWVALLAEARFISLFVIVVSCLFLVF
                     ESTLVQSITVVSVICLTYAGAAWYGVAHLGQQSSLVRDLFMLGCFAPIGVMVSFIARS
                     FRNQRRGLAQAKRQSDEARRELEEAHKALWGEMELAKKIQTVLLPDEPAITGFEIAAY
                     MKPADEVGGDYYDIINVGGRDWVVIGDVSGHGVPAGLIMMIVQTAIKMVLAQRPDIDP
                     SELLTLINRTIAGDIKKISEDKYMTITVLALHQEGRFYFSGLHQDIIVYRGDSGRVET
                     VATDGMWLGIVPEIGDMMRDSTLSIGVGDVMMVFTDGVTEAWRRGSVEGRRDPRADMF
                     GQSRLIEIVERSAVSGPEAVKQAILADLADYQTRDDVTFVILKRVR"
BASE COUNT          719 a         1049 c         1057 g          729 t
ORIGIN      
        1 tggggcgcat tgatggaaca ctgttctttc atgggcacta ctgtggcctt cgtttccgta
       61 ttatcaagga aaaatttcat tcgtggcaca ggtgtggatg cgtgcaatgc acatgcgatt
      121 ttgccttcga tgtgagggaa aagccttcct gaagcgccat ccagtccggt ggtgcatgcg
      181 ctcccggatt cacttcttcg cggttcgttc cgatagtaaa agtaacaaaa ttgttattgc
      241 aaatttgttc ttaagcagtg agttttgggt ttacaagagg tttcgcgtca ctcgaggcag
      301 gagagaattg ccatgacaca gaaaaagggc aaacagttga ataggaatct ggcggccacc
      361 cgtcatgacg agatcttcac caaaataatc accaagaacc acaagcggct cttcatttcc
      421 tgcattccga tttatcttct cgcgaacgtg gctgcggttg ggctcctgat caccggacgt
      481 gcctccaagc acctcacctg ggaggccatc atcctcgagt tctcgatcgc gattcccatt
      541 ctcgcgctta cgtatcttat cgtgaagcgc atgaaggggc gcccgtcgtc cgcctacgtg
      601 gcaatcgcgg gggcgacaat gttctttttt atttttcagt actttatttt cggtacgccc
      661 gaactttttg cgacgcacta catcgtcctc attttctccg tgctctactt caacgagcgt
      721 gtcgcgctct tcgcctttct gatggtggtg ctgtctcaga cgggcctgtt cgccctgcgc
      781 ccggagttgc gccccgacct cgtccccggc gcacacacct cggcgttggt cgtgcgcatg
      841 ttaatctatc tcttcgtggg catcggcgcg accgtgggat ccaaggcctc gcgagagctg
      901 ctgatgctcg ccatcgaaaa ggccgacgag tccgaaaaaa acctgacggg cctgcgccag
      961 gtggtgggcg ccgtcgcgaa atcggtgaag gtgctcaaga gccaggtgga gagccaggac
     1021 aatgtggtca cggagatcaa cgacctgtcg cagaagcaat cggcgtcgct cgaggaggtc
     1081 tcctcgacgc tcgaggagct ctcggggaac tcggagtcca tcaccaagac cgccaaatcg
     1141 cttttcgagg agatggggat cgccaacgat tcggtggagg acctccgcaa ggtctatgac
     1201 gtgatcctca acagcgcctc gtcgatcaac acgtcgatca gcgaggtcac gaaatactcg
     1261 aagcagacct tcgagcagat caagatcatc atggaaaaat tccagatcct gggcgacaag
     1321 gggaccgaca tctcgaactt catccaggtg atcaacgaca tcgccgacaa ggtgaacctc
     1381 ctgtcgctca acgcctccat cgaggcggcg cgggccgggg aatacgggcg cggcttctcg
     1441 gtggtggccg aggagatatc gaagctcgcc gacgccacat cgaagaacgc cagcgagatc
     1501 gaaaagctca tcaacgagaa caagctgctt ctggagggga gccgcgagtt cgtcgaggag
     1561 ttctcccgca tggtgatcca gctcaacaac gccatcggca gcatcaccaa ggagatcggg
     1621 tcggtgggcg acctcatcgt ggacatcggg aacaccgtga agatcatcaa cagcatgggg
     1681 accaagatct acgattccag caagaacatc gagacctcca ccaacgagca gcaggtcgcg
     1741 acggaggaat cgagccggtc ggtgcagtac gtctacgagg cctcggagca gttggtcgcc
     1801 gtggccacaa acatcaccta ttccaccaag accatcaacc ggctcgctga cgacctcatc
     1861 gccctcgtcc agacgatcca tttcgaggag gccgccgctt ccgaatccgc cgagaccgga
     1921 tcagccgcgt aacgccgggt ccgcggtgcg ccgcgatttt gttgttgcga tgaaacatcc
     1981 acgcgctatg ctgatgccat gaatgcgcac tcgacatccc ccgcgtccgc tgacaagctc
     2041 cggcgaatca agcggaccat cattcaaaac atgagctatt cgctcatcaa ttactgcctc
     2101 gcgcccgttc ttaccgtcat cgcggtgtgg atagacctct cttcaatgca ctggtcgcac
     2161 gtagtcgtct tgggcggcgt cgggtcggca gtgaccctcg gctgcatggg gctggtgatg
     2221 gcgcggacgc acatctcgaa tcgcttctcg ctcatgctca tgctggggca gtatatcgtt
     2281 tttctcgcga tcttcgccgt ctgggtcgcg ctcctggccg aggcgcgctt catcagcctg
     2341 ttcgtgatcg tggtttcgtg cctgtttctt gtgttcgaat cgacgctcgt ccagtccatt
     2401 acggtggtca gcgtcatctg cctcacctat gccggggccg cgtggtacgg ggtcgcgcac
     2461 ctcggccagc agtcgtccct cgttcgcgac ctgttcatgc tggggtgttt cgcgcccata
     2521 ggggtgatgg tttccttcat cgcacggagc ttccggaacc agcggcgcgg actcgcgcag
     2581 gcgaagcggc agtcagacga ggcgcggcgc gagctggagg aggcgcacaa ggcgctctgg
     2641 ggcgagatgg agcttgcgaa aaagatacag accgtgctgt tgccggatga gccggccatt
     2701 acgggattcg agatcgccgc gtacatgaaa ccggccgacg aggtgggcgg agactattac
     2761 gatatcatca acgtcggggg gcgtgattgg gtggtcatcg gcgacgtctc cgggcacggc
     2821 gtgcccgccg ggctcatcat gatgatcgtg cagaccgcca tcaagatggt cctggcccag
     2881 cgccccgaca tcgacccctc ggagctgctc accctcatca accgcaccat cgccggagac
     2941 atcaaaaaga tcagcgaaga caaatacatg accatcacgg tgcttgcgct gcaccaggaa
     3001 ggccggtttt atttttcggg cctgcatcag gacatcatcg tctatcgcgg cgattccggc
     3061 cgggtcgaaa ccgtcgccac cgacgggatg tggctgggaa tcgtccccga gatcggcgac
     3121 atgatgcgag acagcacgct ctcgatcggc gtgggagacg tgatgatggt gttcaccgac
     3181 ggcgtgaccg aggcctggcg gcgcggttcg gtcgagggac ggcgcgatcc gcgcgccgac
     3241 atgttcgggc aaagccgcct catcgagatc gtggagcgct ccgccgtgtc cgggcccgag
     3301 gcggtgaaac aggccatcct ggccgacctc gccgattacc agacgcgcga cgatgtgacc
     3361 ttcgttatcc tgaaacgggt gcgatgagcc gataccgtca gtgtcggaac cgctgcgcca
     3421 tgggaatgcg gcggccgatg ccgaaggcct tcgaggttat ctttaagccc ggcggggcct
     3481 gccggcgctt gtattcgttt cggtccaccg tggccagaaa cgaatacaag cgccggcagg
     3541 ccccgccggg ctta
//
LOCUS       DAORHF010000339         3546 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29374_length_3546_cov_4.041822, whole genome shotgun sequence.
ACCESSION   DAORHF010000339 DAORHF010000000
VERSION     DAORHF010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29374_length_3546_cov_4.041822"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            598..834
                     /locus_tag="PKJ16_17925"
     CDS             598..834
                     /locus_tag="PKJ16_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48926.1"
                     /translation="MIDNREQNDNENENRVLLGELMIAGRAINHSQLKAALTVQGILG
                     GRIGEILVTNKCISCEMLDEYLTRQMRIKQGGTD"
     gene            915..2030
                     /locus_tag="PKJ16_17930"
     CDS             915..2030
                     /locus_tag="PKJ16_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48927.1"
                     /translation="MKKKTELAIRKLVTRSIGDAHSPELSLRILSELGIPPTESHLKL
                     LEGADEDGIMVRLGRAAEAWANRPIKDPILNFLNAQDNGANSPGELLPLLGIEIHGFT
                     RHLFETYLNRNSTYELIDAILCEMRSAMRHLADIDRLVQSGVRANLSLHKKLLRLGLH
                     HLDEYLMITRSFDLDEGDLSHAQLFVLGCCCMNEERFKDAMHYFSIAAGIEPDDWRLY
                     YNRAVACDRLEMNQEALNDYTAAIELEENAFQPYVNRGLLRSRLGDLDGAMEDFTAAI
                     DCNAECVEAYVNRGILEASGGNHEYAIQDFSDALSIDPDLATVYCSRAMSYFHLGLKD
                     LAHYDLSVSASLGHSPADELLLKLFSIDNRDTDRSVH"
     gene            2129..2635
                     /locus_tag="PKJ16_17935"
     CDS             2129..2635
                     /locus_tag="PKJ16_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48928.1"
                     /translation="MKPYRTVVACAAFALIAMCAATAIAQTTSFTCSMPAGWNTGTEK
                     DTATMSATSPDGNVHVAVWKVTLSSQDSALNLARRQMQKLKPVKVLAPPTDMSRHAAR
                     FKADSVAKMHFTTLAFNQPDKKPVSYRAFVFVKGKTFVAIEAMATIKATEADFKAADA
                     VIEGFRFN"
     gene            2654..3469
                     /locus_tag="PKJ16_17940"
     CDS             2654..3469
                     /locus_tag="PKJ16_17940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV48929.1"
                     /translation="MGPSPFIVHELTGHIQSIFLVEYPDRILVLDGGCHCDAVLITRF
                     VTRTLRRSMDDVRLAVVTHMHPDHAGGIMLLRRRHHIPVASHAAADSWYAGVGGAIQH
                     VLDIVMAWFVVIRVKSSFRRLWYRRRLAPDHPLEDGSALPGFPDWRAIAVPGHTTHDI
                     ALYHESTGVLYVADLMVQVKSTLTMPIPVSIPELMERSLVRLAALPVRTLLVAHGGRI
                     EHPSPKIFTDLVDHIAARTGIRYALLMLLCGFPAEIRRTKRALAARRPPGERA"
BASE COUNT          735 a         1153 c          994 g          664 t
ORIGIN      
        1 gcgcgcctgc tcgatcttcg cacgcgcggc cttcaggtcc ggatggttct ttcgcgcctc
       61 gatcacgcaa tcctgccagg agtagacgac ctcggcatga agcgagtcgg ccggaagcag
      121 cagcgcgagc gctgcaatca tccctgcgca caacgcccgc aggtgcgttc gcatcacgat
      181 cgagcagcga atgcgagccg accgaacgat atcgcacgat cggcacccat gcggaacggg
      241 cacgacacgt acatggccca ctggccgggg accctgtcca tgcgcccctt catggcgccg
      301 ttttttatcg tcgatgtgca ttgatgattc acgctcgcta ccataagaaa attttcgagt
      361 tttggatcac atcaacccgc aataaacaat ggtgcgcagg tctgatcatt actgcaatca
      421 gtttccgaaa agcatggatt tgtcggaccg ttaacattat tttaaaaatg tttattattt
      481 taaaaaatta gtccagccgt ccgaattttg aatattttta cttgtcaaaa tgccgccctc
      541 tatgatgtat gctcgatcag tgaaacgacc acgcaccgct ccaagcagga ttgccgcatg
      601 atcgacaatc gagaacaaaa cgataacgag aacgagaacc gggtcctctt gggcgagctg
      661 atgatcgccg ggagggcaat caaccattcg caactgaagg cggcgctcac ggtccagggg
      721 atactcggcg ggcgcatcgg agagattctc gtcaccaata agtgcatttc gtgcgagatg
      781 ctcgacgagt atctcacgcg tcaaatgcgc atcaagcagg gcggcaccga ctaacgctct
      841 caagagagac acacgcacat gatcgttcat ccgccccgtg agacgcagga cggtcatccg
      901 cgacatcaaa ccgaatgaaa aaaaagacgg aactcgccat tcgaaaactt gtaacgcgat
      961 ccatcggtga cgcgcattcg ccggagctgt cgttacggat cctctctgag ctgggcatac
     1021 cgcccaccga atcgcatctc aagcttcttg agggcgccga tgaggacggc atcatggtcc
     1081 gcctcggccg cgccgcggag gcctgggcca accgccccat caaagacccg atccttaatt
     1141 tcctgaacgc gcaggacaat ggcgcaaaca gccccgggga gcttcttccc ctgctgggaa
     1201 tcgagatcca cggcttcacc cgccatctgt tcgaaaccta cctcaatcgc aattcgacct
     1261 atgagctgat cgacgcgatc ctgtgcgaga tgcgaagcgc gatgcggcat ctggctgata
     1321 tcgaccgcct ggtgcaatcg ggggtgcgcg ccaatctctc cctgcacaaa aaactgctgc
     1381 ggctggggtt gcaccacctg gacgagtacc tgatgatcac gcgctccttc gatcttgacg
     1441 agggcgacct gtcccacgcg cagctgttcg tcctgggctg ctgctgcatg aacgaagagc
     1501 gcttcaaaga cgccatgcac tacttcagca tcgccgcggg gatcgagccc gacgactggc
     1561 gtctctacta caaccgggcc gtggcctgcg accgtctcga gatgaaccag gaggccctga
     1621 acgactacac cgcggcgatc gagctggagg agaacgcgtt ccagccgtac gtgaaccgcg
     1681 gcctgctgcg ctcgcgcctc ggcgacctgg acggcgcgat ggaggacttc accgcggcga
     1741 tcgactgcaa tgccgaatgc gtcgaggcct atgtgaaccg cgggatcctg gaggcctccg
     1801 ggggcaacca cgagtacgcg atccaggact tcagcgacgc cctctccatc gaccccgacc
     1861 tcgccacggt ctattgcagc cgtgcgatgt cgtacttcca cctgggcctc aaggacctcg
     1921 cgcactacga cctgtcggtc tcggcgtcgc tgggccattc cccggccgat gagctgctgc
     1981 tcaagctctt ttccatcgac aatcgcgata ccgaccgctc ggtccattga cgcgccgacc
     2041 ggcggcagcc ggcatcaccg aaaaaaacct tgccccattt cgcgcacgcc gatacggtat
     2101 gccccgtacg cacgcatagg aggaacccat gaaaccgtat cggaccgttg tcgcatgcgc
     2161 cgccttcgcg ctgatcgcaa tgtgcgccgc caccgcaatc gcgcaaacga catctttcac
     2221 atgctccatg cccgccggat ggaacacagg cacggagaag gacactgcaa cgatgagcgc
     2281 cacctcgccg gacgggaacg tccacgtcgc cgtatggaag gtgacgctgt cgtcgcagga
     2341 tagcgcgctc aatctcgcca ggcgccagat gcaaaagctc aagcccgtga aggtgctcgc
     2401 accccccacc gacatgtcga ggcacgcggc gcgcttcaag gcggattccg tcgcgaagat
     2461 gcacttcacc accctggcct tcaatcagcc cgacaagaaa ccggtttcgt atcgcgcctt
     2521 cgtattcgta aagggaaaga ccttcgtcgc gatcgaggcg atggccacca tcaaggcgac
     2581 ggaggccgat ttcaaggcgg ccgacgccgt catcgaaggc ttcaggttca actgagcggg
     2641 agggcttcct gcgatgggcc catcaccctt catcgtccat gagctcaccg gccacatcca
     2701 gtcgatcttt ctggtcgagt atcccgaccg catattggtc cttgacgggg ggtgccactg
     2761 cgacgccgtc ctcatcacgc gctttgtgac gcgcacgctt cgacgcagca tggacgatgt
     2821 gcgattggct gtcgtcacgc acatgcaccc ggaccacgca ggagggatca tgctgctccg
     2881 gcgacggcac catatccccg tggcctccca tgcggccgcc gacagctggt acgccggcgt
     2941 cggcggcgcc atacagcatg tcttggacat cgtcatggcc tggttcgtgg tgatccgcgt
     3001 gaagagctct tttcgacggt tgtggtatcg acgccgcctc gcgcccgacc atccgctcga
     3061 ggacgggagc gcgcttccgg gatttcccga ctggcgcgcc atcgccgtgc cgggccacac
     3121 cacgcacgat atcgccctgt atcacgagtc gaccggcgtc ctctatgtgg ccgacctcat
     3181 ggtccaggtg aagagcaccc tcaccatgcc catcccggtt tcgatccccg aactcatgga
     3241 gcgatcgctt gtccggctgg cggcccttcc ggtgcgcacg ctgctcgtcg cgcacggcgg
     3301 caggatcgaa cacccgtcgc cgaagatatt cacggacctc gtcgaccaca ttgccgcccg
     3361 aacgggaatt cgctacgccc tgctgatgct cctctgcggt tttcccgccg agatccggcg
     3421 caccaagcgg gcgcttgctg cgcggcgacc cccaggggag cgcgcgtgat attttcgttg
     3481 atttttacgg gctctaccgt acagtagccg gcgcgtaccg gaatcacaat aataatcgag
     3541 aacagg
//
LOCUS       DAORHF010000340         3541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29429_length_3541_cov_5.074871, whole genome shotgun sequence.
ACCESSION   DAORHF010000340 DAORHF010000000
VERSION     DAORHF010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29429_length_3541_cov_5.074871"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="dxr"
                     /locus_tag="PKJ16_17945"
     CDS             complement(<1..1045)
                     /gene="dxr"
                     /locus_tag="PKJ16_17945"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HNV48930.1"
                     /translation="MPATLTILGSTGSIGLSTLRVVDSLDGEYAVYGLACRSNITALE
                     GQIRGYAPRVVAVSADGPETDRAVDALRAKFPDVEFLRGDDGVAELASRPVDVAVSAI
                     VGAAGLRPTMAALGGARRIALANKETLVMAGALFMDEVAARGVELVPVDSEHSAIFAL
                     LASMPRDSLERIILTASGGGLRDHPLDGLSSVTPEEALAHPTWDMGSKITIDSATLFN
                     KGLEVIEAHFLFGVEYERIDVVVHPESIIHSMVELVDGSIHAHLGVADMALPILGSLV
                     HPALRRNPFGRLDLARLGRLSFLPVDPGRYPALALCYAAGRRAGTLPAVLNAANEVAV
                     QAFLHRRIRFTDIA"
     gene            complement(1057..1947)
                     /locus_tag="PKJ16_17950"
     CDS             complement(1057..1947)
                     /locus_tag="PKJ16_17950"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HNV48931.1"
                     /translation="MSSVAKNTITRILTAAIALPVYVFAFTTDRWLNIPILTASAIIS
                     LACLYEYYRVSSVDATKRPFMAAGLVAGLGVNLVMYFYALHAQPGYHAWARPFMDARI
                     IIALVAVLIGVIAAAQLFRRPIQNGIYSLAVTVFGVVFIALFFSHIILIKAVPDGVFH
                     IFFINIVVMLNDTGAYFSGVLLGKHKTNFPVSPNKSWEGYAGGFLISVLSAAATVLIF
                     RSCFGVEIFGVLEAMLIGAALSVTGNLGDLIESAVKRDGSIKDSGSIIPGHGGMWDAF
                     DALVFSMPFYYYYLIVRGLS"
     gene            complement(1944..2660)
                     /gene="uppS"
                     /locus_tag="PKJ16_17955"
     CDS             complement(1944..2660)
                     /gene="uppS"
                     /locus_tag="PKJ16_17955"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HNV48932.1"
                     /translation="MEEIRKLLDPKNIPAHIAIIMDGNGRWAAQHGLSRIEGHRRGAD
                     AVEPIVEAALDIGVQAVSLYAFSTENWSRPKTEVMALWRLLDDFFANKLGRIKERGIR
                     IRHSGSLTGLPPSTKRVIRNSVAETSRNRAMVLNMCLNYGGRREIADAVNAWADTRRA
                     GERITEAKLARHLYTAGMPEVDLLIRTSGECRVSNFLLWQIAYAEFMFTDVLWPDFSP
                     RHLYEMILAFQKRERRFGGL"
     gene            complement(2695..3252)
                     /gene="frr"
                     /locus_tag="PKJ16_17960"
     CDS             complement(2695..3252)
                     /gene="frr"
                     /locus_tag="PKJ16_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HNV48933.1"
                     /translation="MLDEIIADVEDRMKKSISALEKDFVAIRTGRANPAIFDGVKVDV
                     YGSPMPLNQVATISCPEPRLVVINPWDKGNLGEIEKAILKSDLSLNPSSDGNLIRIQI
                     PDLTEERRKEYVKLAKNKAEDCRVAIRNIRRDGNDTIKELEKEKEISEDDSKNYQARV
                     QKITDKYIADVQKLVDNKEKEILNI"
     gene            complement(3316..>3541)
                     /gene="pyrH"
                     /locus_tag="PKJ16_17965"
     CDS             complement(3316..>3541)
                     /gene="pyrH"
                     /locus_tag="PKJ16_17965"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544168.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HNV48934.1"
                     /translation="VDGVYDRDPATDPAAVRLPELTHLDIIQRQLRVMDLTAISLCMD
                     NRMPIVVCDIFHEQSLAKIIHGERIGTLVS"
BASE COUNT          657 a         1132 c         1143 g          609 t
ORIGIN      
        1 gcgcaatgtc ggtgaagcgt atgcgccggt gtaaaaacgc ctgcaccgcg acctcgttgg
       61 ccgcgttgag caccgccggc aaggtgcccg cccgtctccc cgccgcgtaa cacagcgcaa
      121 gcgccggata gcgcccgggg tccaccggaa ggaaggaaag ccgcccgagc cgcgcaaggt
      181 ccagccggcc gaagggattt cgccgcagcg cgggatggac cagcgatcca aggatcggca
      241 gcgccatgtc cgccacgccc agatgcgcgt gaatggaccc atcgacgagc tcgaccatcg
      301 agtggataat gctctcggga tggacgacaa cgtcaatcct ttcgtactcg acgccgaaca
      361 aaaaatgcgc ctcgatcacc tcaaggccct tgttgaacag cgtcgccgaa tcgatggtga
      421 tcttgcttcc catgtcccag gtcggatgcg cgagcgcctc ctccggcgtg acggaggaaa
      481 gcccgtcgag gggatgatcg cgaaggccgc cgcccgaggc cgtgaggatg atgcgctcga
      541 gcgagtcgcg cggcatcgac gcgagcagcg cgaagatggc gctgtgctcg ctgtcgaccg
      601 gcaccagctc cacgccgcgc gcggcgactt cgtccataaa cagcgcgccg gccatcacca
      661 gcgtctcctt gttcgcaagg gcgatccgcc gcgcgccgcc caaggccgcc atggtggggc
      721 gcaatcccgc ggcgcccacg atggccgaaa ccgccacgtc caccggccgg gaggccagct
      781 cggcgacccc gtcatcgccg cgaagaaact cgacatcggg aaatttcgcc cggagcgcgt
      841 cgacggcgcg gtcggtctcg gggccgtcgg cgctcacggc gaccacgcgc ggggcatagc
      901 cgcggatctg cccctcgagc gccgtaatgt ttgagcggca cgcgagcccg tagacggcgt
      961 actcgccgtc cagcgaatcg accacgcgca gcgtcgagag tccgatcgag cccgtcgagc
     1021 cgaggatggt aagcgtcgcg ggcattccgt ttcggctcag gagaggcccc ggacaatcag
     1081 atagtaatag tagaacggca tgctgaagac gagcgcgtcg aaggcgtccc acatcccgcc
     1141 gtgcccgggg atgatcgacc ccgagtcctt gatggagccg tcgcgcttca cggccgactc
     1201 gatgagatcg cccaggttgc ccgtgaccga cagcgccgcg ccgatgagca tcgcctcgag
     1261 tacgccgaag atctcaaccc cgaaacacga ccggaagatg agaaccgtcg ccgcggccga
     1321 gagcacgctg atcaggaagc cgcccgcata gccctcccac gatttattgg gcgacacggg
     1381 aaaattggtc ttgtgcttcc cgaggagcac cccggagaaa taggcgccgg tgtcgttgag
     1441 catcaccacg atgttgatga agaaaatatg gaacaccccg tcggggaccg ccttgatgag
     1501 gatgatatgc gagaagaaga gcgcgatgaa caccacgccg aagaccgtaa ccgcgagcga
     1561 gtagatgccg ttctggatcg gtcggcggaa gagctgcgcc gccgcgatca cgccgatcag
     1621 gaccgcgacg agcgcgatga tgatgcgggc gtccatgaac gggcgcgccc aggcgtgata
     1681 gcccggctgc gcgtgcagcg cgtagaagta catcaccagg ttcacgccca gccccgcgac
     1741 aagccccgcc gccatgaagg gccgcttggt cgcgtccacc gacgacaccc ggtaatactc
     1801 gtagaggcac gcgagcgaga tgatcgccga ggccgtcagg atgggaatgt tgagccaccg
     1861 gtcggtggtg aaggcgaaca cgtataccgg cagcgcgatc gccgcggtca ggatccgggt
     1921 gatggtgttt ttcgcaacgc tgctcatagg ccgccaaacc tccgttcgcg cttctgaaac
     1981 gcgagaatca tttcgtacag atggcgcgga gaaaaatccg gccagaggac gtcggtaaac
     2041 atgaattccg cataggcgat ctgccacagc aggaagttgc tcacgcggca ctccccgctg
     2101 gtgcggatga gcagatccac ttcgggcatg ccggccgtgt acaggtggcg cgccagcttc
     2161 gcctcggtga tccgctcgcc cgcacgccgc gtgtccgccc aggcgttcac ggcgtcggcg
     2221 atctcgcgac gcccgccgta gttgaggcac atattgagca ccatcgcccg gttgcggctc
     2281 gtctccgcga cggagtttcg gataacgcgc ttcgtggagg gcggcaggcc cgtcaacgag
     2341 cccgagtgcc ggatgcggat cccccgctcc ttgatgcgcc cgagcttgtt cgcgaagaaa
     2401 tcgtcgagga gccgccacag cgccatcacc tcggttttgg gccgcgacca gttctccgta
     2461 gaaaacgcgt agagcgaaac ggcctgcacg ccgatatcga gcgccgcctc cacgatgggc
     2521 tcgaccgcgt cggcgccgcg ccgatgcccc tcgatccggg agaggccatg ctgcgcggcc
     2581 catctcccgt tgccgtccat gatgatggcg atatgggcgg gaatgttctt cgggtcgagg
     2641 agtttccgta tctcttccat gactggttgc ggcgtccgcg gggttgcgag cgccctagat
     2701 gttcaggatt tccttttcct tgttgtcgac aagcttctgg acgtcggcga tatacttgtc
     2761 cgtgatcttc tgcacgcgcg cctggtagtt cttggagtcg tcctcggaaa tctccttctc
     2821 cttctccagc tccttgatgg tgtcgttgcc gtcgcggcgg atgttgcgga tcgcgacgcg
     2881 gcagtcctcg gccttgttct tggcgagctt cacgtactcc ttgcgccgct cctcggtgag
     2941 gtccgggatc tgtatccgga tgaggttgcc gtcactcgac ggattgagcg acaggtccga
     3001 tttgaggatc gccttctcga tctcgcccag gttccccttg tcccagggat tgatgaccac
     3061 cagccgcggc tcggggcacg aaatggtggc cacctggttg aggggcatgg gcgatccgta
     3121 cacgtcgacc ttcacgccat cgaagatggc cgggttggcg cgtccggtgc ggatggccac
     3181 gaaatccttt tcgagcgcgg aaatgctctt cttcatgcgg tcttctacgt cggcaatgat
     3241 ctcatcaagc atagtcgtac acctggtcaa tagagagtgg cccggagtgc acacggcgcg
     3301 cccgggcgcg ggagcctacg aaaccagcgt cccgatccgc tcgccgtgaa tgatcttcgc
     3361 gagcgactgt tcgtgaaaga tatcgcacac cacgatgggc atgcggttgt ccatgcacag
     3421 cgatatggcc gtgaggtcca tgacgcgcag ctggcgctgt atgatgtcga ggtgcgtcaa
     3481 ttccggcagg cgtacggccg cggggtccgt ggcgggatcg cggtcgtaca cgccgtcaac
     3541 c
//
LOCUS       DAORHF010000341         3499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_29924_length_3499_cov_4.021487, whole genome shotgun sequence.
ACCESSION   DAORHF010000341 DAORHF010000000
VERSION     DAORHF010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29924_length_3499_cov_4.021487"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2946)
                     /gene="mfd"
                     /locus_tag="PKJ16_17970"
     CDS             complement(<1..2946)
                     /gene="mfd"
                     /locus_tag="PKJ16_17970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HNV48935.1"
                     /translation="MRLIRLAERYAASAEFRTLLDARASGARSLEGIAPSSFPLVIAA
                     LHHHAPTQILVIAKNYQRMEELAGDCASFLDERRVCTFPPWETLPYEFVSPSESIERE
                     RITTIYRIMRGDPVVVIATVESLMRAIPAQSFFTKKGITLTIGEDYPFEDILAALVEY
                     GYRREQRVEAFGQFAVKGGILDIFLPSEANPMRLDFFGDTLESIREFDIDSQVSLDEV
                     EEITVYPRREIILSKAEREALDGKIREAAGRGLETPVIDDADGLPVDGSHAVEGIEEL
                     FPLAIDASTIDTFLRPDAEFVIADTLETMSQCDSLMRSFAELYERRNAHALCLPPETL
                     LRTDAFDAVRARALALNTFSTGIDAVSARLRSVTSFQGKIKTVRGELAERIENGWRAV
                     ICTGFEGQARRLYDLFADLSPSGDFEAIDDASPLNIVITPLREGVEIVDDRTIVITDH
                     EIFGKTYKRKSQFKKKKSRPIDSFLDLREGDYVVHINHGIGIFKGLERMTASGVERDF
                     LLIEYAEGDKLYVTLDQITMVQRYVGLEGKPPRVDLLGKKSAWNRIKEKVQKAIEELA
                     NDLIQIYSQRMALKGFPFPPDTQWQEEFESKFEYEETPDQITAIEDVKDDMESPRPMD
                     RLVCGDVGFGKTEVAIRAAFKSVMAGKQVAVLVPTTVLAMQHFNTFTKRFADYPISVD
                     MISRFRSASEAGDIKRRLTEGALDIVIGTHALLGADVAFKNLGLLVVDEEQRFGVKHK
                     ERLKKLRSLVDVLTLSATPIPRTLHMAVAGIRDLTIIATPPENRQAIDTYVIEENPDI
                     VRDAILREIGRGGQVFYVHNRVETITAQKNILETLVPEATFCVAHGQMEEHELERVII
                     DFLDNRFDVLVSTTIIESGLDMPHVNTIIINRADTFGLSQLYQLKGRVGRSAAKAYAY
                     LFYPRHASLSEIAQKRLQVISEYAELGSGFKIAMKDLEIRGSGNILGREQSGNIMDVG
                     SDLYCQMLED"
     gene            complement(2955..>3499)
                     /locus_tag="PKJ16_17975"
     CDS             complement(2955..>3499)
                     /locus_tag="PKJ16_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48936.1"
                     /translation="QGRAPQCRRSTLDRQSGERHWTLDEGAYDFSIERLIDGEAVTSR
                     HYNYRKYPIRETFVLDTRSPQPTDALPPERLIDYVELARANSVNGYNILFYSGAATVS
                     AGLGALIWFGTDRSLVWMILAGICFGSTAAGYTASGYYTYQYVSTRRALKKIIDLGPR
                     MSLMGGTGPDDIAAGIQLSF"
BASE COUNT          578 a         1057 c         1196 g          668 t
ORIGIN      
        1 gtcctcgagc atctggcagt agagatcgga ccccacgtcc atgatattgc ccgactgctc
       61 gcggcccagg atgtttcccg agccgcggat ctccaggtcc ttcatggcga tcttgaagcc
      121 gcttcccagc tcggcgtatt cggagatcac ctggaggcgc ttttgcgcga tctcgcttaa
      181 tgacgcgtgc cgcggataga acaggtacgc atacgccttg gcggccgaac gccccacgcg
      241 ccccttgagc tgatacagct gcgagaggcc gaaggtgtcg gcccggttga tgatgatggt
      301 gttcacgtgc ggcatgtcca gcccggactc gatgatggtg gtgctgacga gaacgtcgaa
      361 ccggttgtcc aggaagtcga tgatcacccg ctcgagctca tgctcctcca tctgcccgtg
      421 cgccacgcag aaggtcgcct cggggacgag cgtttcgagg atgttcttct gcgcggtgat
      481 cgtctccacg cggttgtgga cgtagaagac ctgtcccccg cgcccgatct cgcgcaggat
      541 cgcgtcgcgc acgatgtcgg ggttctcctc gatcacgtag gtgtcgatcg cctggcggtt
      601 ctccggcggc gtggcgatga tggtgaggtc gcggatgccg gcgacggcca tgtggagcgt
      661 gcgcgggatc ggcgtggcgg acagcgtgag gacgtccacc agcgaccgga gtttcttcag
      721 ccgctccttg tgcttcacgc cgaagcgctg ctcttcgtcc accaccagga gccccaggtt
      781 cttgaaggcc acgtccgcgc ccaggagggc gtgcgtgcca atcacgatgt cgagcgcgcc
      841 ctccgtgagc cgccgcttga tgtcgcccgc ctcgcttgcg gagcgaaagc gcgagatcat
      901 gtccacgctg atggggtagt cggcgaagcg cttcgtgaag gtgttgaagt gctgcatcgc
      961 gagcactgtc gtaggcacga gcacggccac ctgcttgccc gccatcaccg acttgaaggc
     1021 cgcgcgaatg gccacctcgg tttttccaaa gcccacgtca ccgcagacga gccggtccat
     1081 cggtcgcggc gattccatgt cgtccttgac gtcctcgatc gcggtgatct ggtccggcgt
     1141 ctcctcgtac tcgaacttgg actcgaactc ctcctgccac tgggtgtccg gcgggaaggg
     1201 gaagcccttg agcgccatgc gttgggagta tatctggatg aggtcatttg ccagctcctc
     1261 gatggccttc tgcaccttct ccttgatgcg gttccacgcg gatttcttgc cgagcaggtc
     1321 cacgcgcggc ggcttgccct cgagccccac gtagcgctgc accatggtga tctggtccag
     1381 cgtcacgtag agcttgtcgc cctcggcgta ttcgatcagg aggaagtcgc gctcgacgcc
     1441 gctcgcggtc atgcgctcca ggcccttgaa gatcccgatg ccgtggttga tgtgtacgac
     1501 gtaatcgccc tcgcgcagat cgaggaaaga gtcgatgggg cgcgatttct tcttcttgaa
     1561 ctggctcttg cgcttgtagg tcttgccgaa gatctcgtgg tcggtgatta cgatcgtcct
     1621 gtcgtcgacg atctcgacgc cctcgcgaag cggggtgatg acgatgttga gcggagacgc
     1681 gtcgtcgatg gcctcgaaat cgcccgaggg cgagaggtcc gcgaagagat cgtacagacg
     1741 gcgcgcctgt ccctcgaaac cggtgcagat gacggcgcgc cagccgtttt cgatgcgctc
     1801 ggcgagctcg ccgcgcaccg tcttgatctt cccctggaag ctggtcacgc ttcgcaggcg
     1861 tgcgctcacg gcgtcgatgc cggtcgagaa ggtgttgagc gcgagcgccc gtgcgcgaac
     1921 ggcgtcgaag gcgtccgtcc gcaggagcgt ttcgggcggc aggcacaggg cgtgtgcgtt
     1981 ccggcgctcg tataattcgg cgaaggagcg catcaggctg tcgcactggg acatcgtctc
     2041 gagggtgtcg gcgatgacga actcggcgtc cgggcgcagg aaggtgtcga tggtgctcgc
     2101 gtcgatcgcg agcgggaaga gctcctcgat gccctcgacg gcatgggacc cgtccacagg
     2161 gagaccatcg gcatcatcga tgacgggcgt ttcgagaccg cgtcccgccg cctcgcggat
     2221 cttcccgtcc agcgcctcgc gctcggcctt cgagaggatg atctcgcgac gcgggtacac
     2281 ggtgatctcc tcgacctcgt cgagagagac ctggctgtcg atgtcgaact cgcggatgga
     2341 ctcaagcgtg tcgccgaaga aatccaggcg catggggttc gcctcggagg gaagaaagat
     2401 gtccaggatg ccgcccttga cggcgaactg gccgaaggcc tccacgcgct gctcgcgccg
     2461 atagccgtac tccacgagcg ccgcgaggat gtcctcgaac gggtagtcct cgccgatggt
     2521 gagcgtgatc ccttttttcg tgaagaagga ttgcgcgggg atggcgcgca tgagcgactc
     2581 gacggtcgcg atgacgacga cgggatcgcc gcgcatgatg cgatagatgg tggtgatccg
     2641 ctcgcgctcg atggattccg acggcgagac gaactcatag ggcagcgttt cccacggcgg
     2701 gaacgtgcac acgcgccgct cgtcgaggaa ggaggcgcaa tcgcccgcga gctcctccat
     2761 gcgctggtaa tttttggcga ttaccaggat ttgcgtgggg gcatggtgat ggagcgcggc
     2821 gatgaccagc gggaacgagg acggcgcgat cccctcgagc gagcgggcgc cggatgcgcg
     2881 cgcgtcgaga agggtgcgga attccgccga ggcggcgtag cgctcggcca ggcgtatcag
     2941 tctcatgggg ccggttaaaa ggacagctgg atgccggcgg cgatatcgtc ggggccggtg
     3001 ccacccatca gcgacatccg ggggccgagg tcaatgattt ttttgagcgc tcgccgcgtg
     3061 gagacgtact ggtaggtgta gtaaccggac gcagtgtagc ccgcggcggt cgagccgaag
     3121 cagattccgg cgaggatcat ccagacgagg ctgcggtccg tgccgaacca gatgagcgcc
     3181 cccaatccgg cggacacggt ggctgcgccg gagtagaaga ggatgttgta tccgttgacc
     3241 gaattggcgc gcgcgagttc cacgtagtcg atgagccgtt ccggcgggag cgcgtcggtc
     3301 ggctgcggcg agcgcgtgtc caggacgaag gtttcccgga tgggatactt gcggtaattg
     3361 tagtgccgcg aggtcacggc ctcgccgtcg atgaggcgct cgatggaaaa atcgtaggcg
     3421 ccctcgtcga gcgtccagtg ccgctcgccg ctctgccggt cgagcgtact tcgtcggcat
     3481 tgaggcgcac gaccttgcc
//
LOCUS       DAORHF010000342         3493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30003_length_3493_cov_4.200116, whole genome shotgun sequence.
ACCESSION   DAORHF010000342 DAORHF010000000
VERSION     DAORHF010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30003_length_3493_cov_4.200116"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2470)
                     /locus_tag="PKJ16_17980"
     CDS             complement(<1..2470)
                     /locus_tag="PKJ16_17980"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HNV48937.1"
                     /translation="MGFIAWYSRKRRDTVAGAGVYQWIAFLASLLSIFQGLSMIGFSS
                     EWARFWYDMRIVCFAVIPVLWLVFVLYFVGKSEWISKVRIAVLFIIPVVTQVMLWTND
                     MHGLWVIHNVSFRSAGPFFIPVLESRVPGPWFVVHNIYTYGMMMVGFVILFVLSVRLQ
                     SLYRGQVVALGTGTLIMMIGALVPTLNLFPGMELNPLPQCFAIGSLVIAWGLYRHQFL
                     VASPKYDDDKRIPGVIVMLFVIVTVGILSVGFLYYRQHQNQFRAEVGRTLSSIAELKV
                     SELVQWRKERLADADSLHGNIAFANLMRRIISNPADGDAYRQMRTWLEKIRASYRYDH
                     VVLLDVRGTARLSVPQSVEPLCANVRQQTAEIMRTGRITFLDFHQEAPGKPIHLAVVV
                     PIVEQGHPMGLVALFIDPTAYLFPLLNRWPTPSRTAETLLVRRDGNDVLFLNELRFRK
                     NTALSLRIPLVDERVPAVMAALGREGVVEGIDYRGVPVAAAIRAVPGSPWVMVARMDL
                     AEVYAPLREWLWLMIVLVCAILAGAGSGVWLIWRRQSDRALKEKVETAELLRASENRY
                     RRLFESAKDGILILDVVTGMIVDVNPYMIEMLGFSQEQFREKTIWDIGFLGDIIPNKE
                     KFRELQEKGYVRYEGLPLETSDGRRIDVEFISNVYEIARGKVIQCNIRDISERIRMQR
                     ALLGVTSHQEALLSAIPDIVMEVDVNKVYTWSNGPGFEFFGDDVIGREAAYYFEGEQD
                     TYRSVQPLFNGAEETIYLESWQRRRDGERRLLAWWCRVLKDEFGKVTGALSSARDVTE
                     SRQAEEAIRESEDKFRGIFDGSTVG"
     gene            complement(2549..>3493)
                     /locus_tag="PKJ16_17985"
     CDS             complement(2549..>3493)
                     /locus_tag="PKJ16_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HNV48938.1"
                     /translation="LYYSLLGYDNEEFPATYASWRLLVHPDDIDRVERDLSQSIETGK
                     GFSIDLRMRMKSGEWRWVCTRGKTVAWDQRNKVRRMVGTLTDITERKQAEEYLGALSC
                     ERELLLREVHHRIKNNMSMIASLLSLQASRVSDPSSAFALNDSRGRVISMAVIYDLLY
                     KTSDFRYVPVREYLGRIVDGITASLPSIPGVAVEKRIDDFVMDSKILFPIGIIINELV
                     TNAFKYAFPDDRSGMIAIAVARLPENRVEITVRDDGVGIPDSVMSNGHTGFGMELVAL
                     LVKQIRGSFECVREGGTVFRIGFQYVAGEGNDDVLNGE"
BASE COUNT          807 a         1072 c          902 g          712 t
ORIGIN      
        1 tccccacggt tgaaccatcg aatatgccgc ggaacttgtc ttcgctttcc ctgatcgcct
       61 cttccgcctg cctgctttca gtgacatccc gggccgacga gagggccccg gtaaccttcc
      121 cgaactcatc cttgaggacc ctgcaccacc aggcgagcag tcgccgctcc ccgtccctgc
      181 gccgttgcca gctctcgagg tagatcgttt cttctgcgcc gttgaacagc ggctgcaccg
      241 atcggtacgt atcctgttca ccttcgaaat aataggccgc ctccctgccg atgacatcgt
      301 cgccgaaaaa ctcaaatccc ggcccgttgc tccacgtata caccttgttg acgtcgacct
      361 ccatgacgat gtccggtatg gccgacagca gggcctcctg gtgcgaggtg acccccagca
      421 gggccctttg cattctgatt ctttccgaga tatccctgat gttgcactgg atcaccttac
      481 cccgggcgat ctcatatacg ttactaatga attccacgtc gattctgcga ccatccgagg
      541 tttccagcgg caggccttca tatcgcacat atcccttttc ctgtaattcc cgaaattttt
      601 ctttattggg gatgatgtcc ccgagaaatc cgatgtccca gatcgttttc tcccggaact
      661 gctcttgcga aaaaccaagc atctcgatca tataagggtt cacatccacg atcattcccg
      721 tcacgacatc gaggatgaga atcccgtcct tggccgactc aaaaaggcgg cgatatcggt
      781 tttccgaagc gcgaagaagc tccgcggtct ccaccttctc ctttaacgca cggtcgctct
      841 gcctgcgcca gatcaaccag acgcccgatc ctgctcccgc gagaatcgcg catacgagaa
      901 cgatcatcag ccacagccac tcccgcagcg gcgcatacac ctccgcaagg tccatgcggg
      961 cgaccatgac ccatggtgaa ccgggaacgg cgcggattgc ggcggcaacc ggcacacccc
     1021 ggtaatcgat gccctccacc acgccttcgc gccccagcgc tgccatcacg gccgggactc
     1081 tttcatcgac cagcggaatg cggaggctta gggcggtgtt ttttcggaaa cgaagctcat
     1141 ttaaaaacaa aacgtcattg ccgtcgcggc ggacgagcag cgtctcggcg gttcgactgg
     1201 gcgtgggcca gcgattgagc aacggaaaga gataggcggt gggatcgatg aacagcgcga
     1261 caaggcccat cgggtgcccc tgctcgacta tcggcacgac aacagcaagg tggatcggct
     1321 tccccggcgc ctcctggtga aaatcgagga aggtgatcct gcccgtacgc atgatctccg
     1381 ccgtctgctg ccgtacattc gcgcacaacg gttcgacgga ttgcggtact gacaaccggg
     1441 ccgttccccg cacatcgagc agaacgacat gatcataccg ataggatgcc cgtatttttt
     1501 cgagccaggt gcgcatctgc ctgtacgcgt cgccgtcagc cggattggat ataatccgcc
     1561 gcatgaggtt ggcaaaagcg atattcccat gcaaagaatc ggcgtcggcc agccgctcct
     1621 ttctccactg caccaactcg ctcactttga gctcggcaat ggaggagagc gtgcgcccca
     1681 cctcggccct gaattgattt tgatgctgcc ggtaatagag aaatcccacc gagagaatac
     1741 cgacggttac aataacgaac agcatgacga tcacgcccgg tattcgtttg tcatcatcat
     1801 atttcggcga tgcgacaagg aattggtggc gatagaggcc ccaggcgatt acaagcgagc
     1861 cgatcgcgaa gcattgcggc agcggattga gctccatgcc cgggaaaagg ttgagcgtgg
     1921 ggaccagggc tccgatcatc ataatgagcg taccggtgcc gagtgcgaca acctgccccc
     1981 ggtacaggct ttgcaggcgt actgacaaaa cgaagagaat gacgaatccc accatcatca
     2041 tcccgtaggt atagatgtta tgcaccacga accacggacc gggaactcga gactctaata
     2101 caggaatgaa aaacggaccc gccgatcgga aactcacgtt atgaatcacc cacagcccgt
     2161 gcatatcgtt cgtccagagc attacctgcg ttacaacagg aataatgaag agcacggcga
     2221 tacgtacttt cgaaatccat tcggatttcc cgacaaagta tagtacgaat accagccaga
     2281 gcaccggtat cacagcgaag cacacgatcc gcatgtcgta ccagaagcgg gcccactccg
     2341 acgagaaacc gatcatcgac aatccctgga agatggacag gaggctggcc agaaacgcga
     2401 tccactgata cacgccggcc ccggcgacgg tatcccgccg cttccgggaa taccacgcga
     2461 tgaatcccat caccgcgacc gcgatgatga tggaaatgaa gtaggtgatg tgctgccagt
     2521 tcattgcctg taccgccttc gatgcgaatc actcaccgtt taaaacgtcg tcattgcctt
     2581 ctcccgcgac gtactgaaaa ccaatccgaa aaaccgtccc gccttcgcga acgcattcga
     2641 acgatccccg gatctgctta actaggagcg ccaccagctc cattccaaaa ccggtgtggc
     2701 cgttactcat taccgaatcg gggatgccaa cgccgtcgtc gcgaacggtt atttctacgc
     2761 ggttttccgg cagacgcgcc accgcgatgg caatcatccc gcttctgtcg tccgggaacg
     2821 cgtacttgaa cgcgttggtt accagctcgt taatgatgat gccgatcgga aaaagaatct
     2881 tcgaatccat gacaaaatca tcgatccgct tttccaccgc aacccccggg atggagggca
     2941 acgatgcggt aatgccgtcc acgatcctgc cgaggtattc tctgacggga acatatctga
     3001 aatcgctcgt tttatacagc aggtcataga tcacggccat gctgatcacc ctgccacggg
     3061 agtcgttcaa tgcgaacgcg gacgaaggat cggacacccg cgatgcctga agcgaaagga
     3121 ggctcgcgat catggacatg ttgttcttaa tacggtgatg cacctcgcgt aggagaagtt
     3181 cgcgctcgca agacagagcg ccgagatatt cctcggcttg cttacgctca gtgatatcag
     3241 tcagggttcc caccatgcgc cgaaccttgt tccgctggtc ccaggcgacg gtcttgcccc
     3301 tggtgcatac ccatctccac tcgccggatt tcatccgcat acggaggtcg atggagaatc
     3361 ccttgccggt ttcaatactc tgcgagagat cgcgctcgac acgatcgata tcatccggat
     3421 gaacaagcaa ccgccatgac gcgtaggtgg cgggaaactc ctcattgtcg tagccgagca
     3481 acgagtagta gag
//
LOCUS       DAORHF010000343         3466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30380_length_3466_cov_5.421284, whole genome shotgun sequence.
ACCESSION   DAORHF010000343 DAORHF010000000
VERSION     DAORHF010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30380_length_3466_cov_5.421284"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1365)
                     /locus_tag="PKJ16_17990"
     CDS             complement(214..1365)
                     /locus_tag="PKJ16_17990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HNV48939.1"
                     /translation="MIGAETLIRVLQKYRLHRPVSEADQARIERAGARTLRLILRQET
                     AGPMRSTAVALLCALRGMGVAITPAWSGRLANAALAACAIVVVGAASVALRSYVYDPH
                     MAARDLAVGSRGSVLFVIGDATIRSQDAQPRRAAVRDSIVRGDVLQTGPASQVAVQIG
                     SHVVFTLEPDTSVAVTALFESSGARLDLHRGTILSKVMKLGKDARYAIATTNAVAAVR
                     GTGFSVAYEPVKTTVSVSEGAVLVRFVAPATGAVKDEKELGPGSTAIVTDRLEIRDAE
                     RAEGLRLGKLAALRFIPVEAPGDERALADLAAQYRRAAEDADKKISGDTERPLTLDEL
                     RAKYNRIDTVRLYNGKVITGVIVSRGATYRILTPAGTVSVSQDDIKSTK"
     gene            complement(1362..1901)
                     /locus_tag="PKJ16_17995"
     CDS             complement(1362..1901)
                     /locus_tag="PKJ16_17995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48940.1"
                     /translation="MSQANSIGSERTRRYTEDFTAHYSALFSAVHSRIGNFQEAEDIC
                     QEVFLRLYRKYDEVEDPRRWLYGAMRIVMLDYFKAKGRSDIDVDSILDDASLAYVNGF
                     RDTRLLLQEAMEAIYTDEGEREWVLFNLIAIHGFSLVQAGRHLKISYKQVRYGYEKAM
                     KKLLAHLNARGIRSLEDLL"
     gene            complement(2631..2885)
                     /locus_tag="PKJ16_18000"
     CDS             complement(2631..2885)
                     /locus_tag="PKJ16_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48941.1"
                     /translation="MKKIIIASLFVPLMLLVAIGIMARSDQKNAPDTRTPLEKCYDRC
                     KDSHRRYVGHSPAQNIVKAGKVKLDACKKSCDQTGKPKNF"
     gene            complement(3142..>3466)
                     /locus_tag="PKJ16_18005"
     CDS             complement(3142..>3466)
                     /locus_tag="PKJ16_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HNV48942.1"
                     /translation="RDPQFGPCVMFGLGGVFTEVLRDTTFRVAPLSREDAAEMLDEVK
                     ARALLGEFRGQPAVDREALIDALVGIGALALENDEIAEIDINPIIVAGDRPVAVDAVV
                     VLRRE"
BASE COUNT          732 a         1071 c          938 g          725 t
ORIGIN      
        1 cggctggatt aagaatgttg cttaactgcg tgtccgcaaa atcggggcaa gcccagggga
       61 aaggggccgg gggagagggg tataataata acgcaaccct ccggatgtcc tttaacaaca
      121 tccgtccgaa acaatgcgta ccagtacgtt cactggtttc cctctagtag gaaaattgtc
      181 cgggatttgc ccaaaaaaat cgaaatcttt gttttatttc gtactcttga tatcgtcctg
      241 tgagacgctg accgtcccgg caggagtcag aatacgatag gtcgctccgc gagacacgat
      301 aacgcccgtg atgacctttc cgttgtacag gcgcaccgta tcgatgcgat tgtatttcgc
      361 gcggagctca tcgagcgtga gcggcctctc tgtgtcgccg gaaatcttct tgtcggcatc
      421 ctccgccgcg cgccggtact gcgcggccag gtccgcgagc gcccgctcgt cgcccggcgc
      481 ctcgaccgga atgaagcgca gggcggcgag ttttccgagc cgaagccctt ccgcgcgctc
      541 cgcatcgcgg atctccagcc ggtcggtgac gatcgccgtc gatcccggtc ccagttcttt
      601 ctcgtccttg acggccccgg tcgcgggcgc cacgaaccgt acgagcaccg caccctcgga
      661 caccgagacc gtggttttca ccggctcata cgccacggaa aagcccgttc ccctgaccgc
      721 cgcgaccgcg ttcgtcgtcg cgatcgcata ccgcgcgtcc ttcccgagct tcatcacctt
      781 cgagagtatc gtaccgcgat gcagatcgag ccgcgcgccg gacgattcga agagcgccgt
      841 caccgcgacc gacgtgtccg gctccagcgt gaaaacgaca tggctcccaa tctgcaccgc
      901 cacctgcgag gcgggtccgg tctgaagaac atcgccccgc acaatgctgt cccgcacggc
      961 cgcgcggcgc ggttgtgcgt cctggctccg gatggtcgca tcgccgatga cgaacaatac
     1021 ggaaccccgc gagcccacgg ccagatcccg cgccgccatg tgcggatcat acacgtacga
     1081 tctgagcgcg acggacgcgg cgccgacgac gacaatcgcg catgcggcaa gcgcggcgtt
     1141 cgccaatcgg cccgaccatg ccggcgtgat cgcgacgccc atcccccgaa gcgcgcacaa
     1201 cagcgcgacc gccgtcgaac gcatgggacc cgcggtttcc tgccggagaa ttaaacgcag
     1261 ggtgcgcgcg cccgcccgtt cgatccgcgc ctgatcggcc tccgacacgg gccggtggag
     1321 ccggtatttc tgcaatacgc gaatgagcgt ctcagcgccg atcacagcag atcctccagg
     1381 ctcctgatgc cgcgcgcatt gagatgcgcg agaagctttt tcatcgcctt ctcgtagccg
     1441 tagcgaacct gtttgtagga aatcttcaaa tgcctccccg cctggaccag tgaaaaccca
     1501 tggatcgcga taagattgaa caggacccac tcccgctcgc cttcgtccgt atagatggct
     1561 tccatcgcct cctgcagcag cagccgcgtg tcacgaaagc cgttcacgta ggccagcgac
     1621 gcgtcgtcga gaattgaatc gacatcgata tcggaccggc cttttgcctt gaaatagtcc
     1681 agcatgacga tgcgcatggc gccgtacagc cacctgcgcg gatcctccac ttcgtcgtac
     1741 ttgcgatata gccggagaaa cacctcctgg cagatgtcct ccgcctcctg gaagttcccg
     1801 atgcgcgaat ggaccgcgct aaacagcgcg gagtaatgcg ccgtgaagtc ctcggtgtac
     1861 cgtcgcgttc gctcggagcc tattgaattg gcctgggaca cgtgccctct atattgctaa
     1921 cgtagcggat ttgctggaaa agtgcccaca aaaacgaatg tcgcgggcat ttttacgctc
     1981 aaatggtact agatttctga atttgtccag ttgaaattta ccagcaagaa aggtgcggcc
     2041 ttttttcgta aggaatcaag agacatgctg cgggttgtat tggatcaacc ctgcaggcag
     2101 tacccccttg gcttcgatac gcctgcggtt actcagccag cggggggcta cttccacggt
     2161 agttcgttac ttaattattc cgcgagctga atacctgcag ggatacttga acagcctgat
     2221 ggctcaatag ccaacaacat gccagaattg ctcggtggca gagtggttta caacgtacca
     2281 gaataacacg gtggctgagt agccgcaggc gtatcgaagc caccgtgcca gcagtgtgcg
     2341 aacagtcaac gcctatcttg gtattgtgtt acgggtgaaa tacagcggtg gcaaaacctt
     2401 catggggcac atcaacaaac agtcctcata atggtgctgc cacatcccgc atcggtccat
     2461 tccaagcgaa aaagcccttt atatcggcct tataatttac atgcagaccg aaaaaggaac
     2521 acgagagaga ttgtcagcgg ggatcgctgg aggaatcaat tacggtgagg ggctcccggc
     2581 ccgattgggg gaatccgggc cgggaatgag ttgtttcagc agcactatgc ttaaaaattc
     2641 tttggctttc cggtttggtc acatgacttt ttgcatgcat ccagtttaac ctttcctgcc
     2701 ttgacgatat tttgcgcagg actgtggcca acatacctcc gatgactgtc cttgcaccgg
     2761 tcatagcatt tctcaagcgg ggtccgggta tcgggggcgt ttttctggtc actccgcgcc
     2821 atgatgccta tcgcgactaa taacatgagg ggcacaaaca ggctggcaat aattattttc
     2881 ttcatgctgt tcctccaaaa tcttttttta catgaatcct atcacaatcg tgcaatggca
     2941 taacggacac tgtcaagatt attttaactt tcaagggaat tgcgaccttt tgtgaaatct
     3001 gatacgggac acaggttccg ttatcagcaa cggtcacatc ccgcaaccag ccccgtccaa
     3061 gcccaaaaag tcctttttac cggccttata ttttcctact gcaactacta tcgtatttca
     3121 ggaaactagt ttcaacttcc gtcactccct ccgcagcacc accaccgcat ccaccgcgac
     3181 cgggcggtcg cccgcgacga tgatcgggtt gatgtcgatc tcggctatct cgtcgttctc
     3241 cagcgcgagc gcgccgatcc ccaccagcgc gtcgatgagc gcctcgcggt ccacggccgg
     3301 ctggccgcgg aactcaccca ggagcgcgcg cgccttcacc tcgtcgagca tctccgcggc
     3361 gtcctcgcgc gaaagcggcg ccacgcgaaa ggtcgtgtcg cgcagcacct cggtgaagac
     3421 gcccccgagg ccaaacatca cgcagggccc gaactgcggg tcgcgc
//
LOCUS       DAORHF010000344         3460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30456_length_3460_cov_4.372981, whole genome shotgun sequence.
ACCESSION   DAORHF010000344 DAORHF010000000
VERSION     DAORHF010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30456_length_3460_cov_4.372981"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /gene="hisH"
                     /locus_tag="PKJ16_18010"
     CDS             <1..495
                     /gene="hisH"
                     /locus_tag="PKJ16_18010"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HNV48943.1"
                     /translation="SALVLPGDGAFGRAMENLARQGWIEPLMRFIEAGGVFMGICLGF
                     QLLFSTSEEFGAHRGLDLIPGDVIRFRANGLKVPHMGWNRVALAGASKFLDGIAPESF
                     FYFIHSYHPVTAAREWILGEAEYGVRFPCIVGRGNVIATQFHPEKSHRVGLKIVENFV
                     RAAC"
     gene            489..1208
                     /gene="hisA"
                     /locus_tag="PKJ16_18015"
     CDS             489..1208
                     /gene="hisA"
                     /locus_tag="PKJ16_18015"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019421946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HNV48944.1"
                     /translation="MLVIPAIDLKNGRCVRLVQGDPDRETVYSDDPVSVARRFVDAGA
                     RLIHVVDLDGAFQGVPVNAGIVEEIARAVPIPIEIGGGIRSAQTIERYLKAGIGRVIL
                     GTAVIEGDFDAMLAAYGDAIIVGLDARDGMVATRGWKAVSSLPVADCIDRLKEKGIKE
                     IIFTDISTDGMLSGPNYTSLENVLSRAGAIAVIASGGISALDDIRRLAAYRDPAVKGC
                     IVGKSIYDGRIDLADAIEAAR"
     gene            1263..2327
                     /gene="dgcA"
                     /locus_tag="PKJ16_18020"
     CDS             1263..2327
                     /gene="dgcA"
                     /locus_tag="PKJ16_18020"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014454926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcA"
                     /protein_id="HNV48945.1"
                     /translation="MNSDQHGKGLEIKLPIEIEQYERKIYDLKQLIEISKGLNSTLDY
                     NILIDSILLTCMGQMQLLKAGIFTKKEISHDLFVLHRNYKGFEIDHSLEYGIPVDSAL
                     VRFLESGGRCYEMSEILKMMGDDPSLETFKIIDPCLIVPLKGKGRLNGIIILGERINT
                     SKFLESEKEFLLNIASLAGIAIQNAYLFELATTDMMTGLKIHHFFQNSLMEERERALA
                     NKTPLSLIMMDIDHFKNFNDSYGHLCGDQVLINVARLTMDNIRQLDLAARYGGEEFII
                     LLPNTEITEARAVAERIRKSIEGARVECGQNALGVTVSLGVAQFDPERDMSNLNLIER
                     ADKAMYLSKEGGRNRISVLT"
     gene            2336..2917
                     /locus_tag="PKJ16_18025"
     CDS             2336..2917
                     /locus_tag="PKJ16_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48946.1"
                     /translation="MASAKPRTGSTRPKRLIRSMAFLLAAVLVFNTGAVPAVFAVTVE
                     EEAGRKGKAPRRDYEYDSDFRRKVPERREREYERGEKDPRLACLLSIIVPGGGHVYLN
                     REFKGVAFCLTTVSFYGAAVYFYLRSTNSDLSETESRSNAVIAGVLLVIAAIFHTVGI
                     VEAYNDAIDVNEKRYYYGPRRSRNPYVGDIEKE"
     gene            2980..3053
                     /locus_tag="PKJ16_18030"
     tRNA            2980..3053
                     /locus_tag="PKJ16_18030"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3014..3016,aa:Ile,seq:cat)
     gene            3114..3425
                     /gene="rplU"
                     /locus_tag="PKJ16_18035"
     CDS             3114..3425
                     /gene="rplU"
                     /locus_tag="PKJ16_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HNV48947.1"
                     /translation="MYAIVEISGKQYRVEKDKTIEVDRLDVGADTMTIDKVLLCANGD
                     TVMVGQPYLSNVKISATVLGGIKGKKVVGVKFKKRKNYTRTKGERAELTRLKIGEIAV
                     Q"
BASE COUNT          729 a          994 c         1056 g          681 t
ORIGIN      
        1 agcgcgctcg tgttgccggg cgacggcgct ttcggtcgcg cgatggagaa ccttgcgcga
       61 cagggatgga tcgagccgct catgcgcttc atcgaagcgg gcggcgtttt catgggaatt
      121 tgcctgggct tccagctcct cttttccacc agcgaggagt tcggcgcgca ccgggggttg
      181 gatctgatac cgggcgacgt gattcgtttc cgcgccaatg gcctgaaggt cccgcacatg
      241 ggctggaacc gtgtcgcact ggcgggcgcg tcaaaattcc ttgacgggat tgccccggaa
      301 tcgttttttt acttcataca ttcatatcac ccggttaccg cggcgcgtga atggatcctg
      361 ggcgaggcgg agtacggcgt ccggtttccc tgcattgtgg gacggggaaa cgtgatcgcg
      421 acgcagttcc atccggaaaa aagccatcgc gtgggattaa aaattgttga aaatttcgtg
      481 agagcggcat gttagtaata ccggcgatag acctgaaaaa cggtcggtgc gtgcggctgg
      541 tccagggcga tcccgatcgc gagaccgtgt attcggacga tcccgtaagc gtcgcgcggc
      601 gtttcgtcga tgccggcgcg cggctcatcc acgtggtcga tctcgacggc gcgttccagg
      661 gcgttccggt gaatgcgggc atcgttgagg agatcgcccg cgcggttcca attcccatcg
      721 agatcggggg cggcatccgc agcgcgcaga cgatcgagcg atacctcaag gccggcatcg
      781 gccgggtgat actcgggacc gcggtcatcg agggcgattt cgacgccatg ctcgccgcgt
      841 atggtgacgc gatcatcgtc gggctcgatg cgcgcgacgg catggtcgcc acgcgcgggt
      901 ggaaggcggt ctcgtcgctc ccggtcgccg attgcatcga tcggcttaaa gaaaagggaa
      961 taaaagagat catttttacc gatatttcta ccgacggcat gttgagcggg ccgaactata
     1021 catctcttga aaatgtattg tcgcgggcgg gcgcgatcgc cgtaatcgca tcaggcggga
     1081 tatccgcgct cgacgacatc cgacgccttg ccgcgtaccg cgatccggcc gtcaagggat
     1141 gtatcgtcgg gaagtcgata tacgatggcc ggatcgatct cgccgatgcg attgaagcgg
     1201 cgcggtagcg ggacgggacc gatgaaacct tttgccgtaa gcggagtcat actggtgcaa
     1261 ccatgaacag cgatcagcac ggaaaaggac tggagatcaa gctccccatc gagatcgagc
     1321 aatacgagcg caagatctac gacctcaagc agctcatcga gatcagcaag gggctcaatt
     1381 ccacgctcga ctacaatatc ctcatcgact ccattctgct cacctgcatg gggcagatgc
     1441 agcttttgaa ggcggggatc ttcaccaaga aggagatcag ccacgacctg ttcgtgctcc
     1501 accgcaacta caaggggttc gagatagacc attcgttgga gtacggcatc cccgtcgatt
     1561 cggcgctggt ccgcttcctg gagagcgggg gccgctgcta cgagatgagc gagatcctca
     1621 agatgatggg cgacgatcct tcgctcgaga cgttcaagat catcgaccca tgtctcatcg
     1681 ttccgctcaa gggcaagggg cggctcaacg gcatcatcat cctcggggag cgcatcaata
     1741 ccagcaagtt cctcgagtcc gagaaggaat tcctgctcaa catcgcgtcg ctcgcgggta
     1801 tcgccatcca gaacgcgtat ctcttcgagc tggccaccac cgacatgatg accggcctca
     1861 agatccatca cttcttccag aactcgctca tggaggagcg cgaacgggcg ctggcgaaca
     1921 agacgccgct ttcgctcatc atgatggaca tcgaccactt caagaacttc aacgactcct
     1981 acgggcacct ctgcggggac caggtgctca tcaacgtggc ccgacttacc atggacaata
     2041 tccgccagct cgatctcgcg gcgcgctatg gcggcgagga gttcatcatc ctgctgccca
     2101 ataccgagat caccgaggcg cgcgccgtcg cggagcgcat ccgtaagagc atcgagggag
     2161 cccgggtcga gtgcggacag aatgccctcg gcgtcacggt atcgctgggc gtggcgcagt
     2221 ttgatcccga gcgcgacatg tcgaacctca acctcatcga gcgtgccgac aaggcgatgt
     2281 atctctccaa ggagggcggc cgaaaccgca tctcggtttt gacctgatcg atccaatggc
     2341 atcagcgaaa ccgcgtaccg gatcaacccg accgaaacgg ctcattcgtt cgatggcgtt
     2401 cctgcttgcc gccgtgctgg tgttcaatac cggcgcggtg cccgccgtgt tcgccgtcac
     2461 ggttgaggag gaggccggtc ggaaggggaa ggccccccgc cgcgactacg agtacgattc
     2521 ggacttcagg cgcaaggtgc cggagcggcg cgagcgcgaa tacgagcgtg gcgagaagga
     2581 cccgcgcctg gcctgcctgc tgtcgatcat cgtgcccggc ggcggccatg tgtacctgaa
     2641 ccgcgagttc aaaggggtcg ccttctgcct aacgacggta tcgttctacg gggcggccgt
     2701 ttacttctac ctgcgttcca ccaacagcga cctttcggaa acggagtccc ggtcgaatgc
     2761 cgtcatcgcc ggcgtccttc tggtgattgc cgccatattc cacacggtgg gcatcgttga
     2821 ggcgtacaac gacgcgatcg acgtgaacga aaagcgctat tactacggtc cgcgtcgttc
     2881 tcgcaatccc tatgtgggcg atatcgagaa agagtgacat ttttcgatat ttccttgaca
     2941 atttccaccc ccatccttct agttggtatg ctttaaaacg ggcccatagc tcagtcggtt
     3001 agagcggctg actcataatc agtaggtcca tggttcaatt ccatgtgggc ccacactgtt
     3061 cgcatgtgct cacgggaacg cgcgaaacat caatcggata agggacgaaa cgcatgtatg
     3121 caatagtgga gatcagcggc aagcaatata gggtcgagaa ggacaagacc attgaggtgg
     3181 accggcttga tgtaggcgcc gatacgatga ccatcgacaa ggtgcttttg tgcgccaacg
     3241 gcgacaccgt catggtgggt cagccgtatc tcagcaatgt gaagatcagc gccacggtgc
     3301 ttggcgggat caagggcaag aaggtcgtcg gggtgaagtt caagaaacgc aaaaactaca
     3361 cccgcaccaa gggcgagcgg gccgagctca cgaggctcaa gatcggcgag atcgccgttc
     3421 agtaaggcgt ggtcgcagtc catttccgca acatgacccc
//
LOCUS       DAORHF010000345         3457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30492_length_3457_cov_4.267784, whole genome shotgun sequence.
ACCESSION   DAORHF010000345 DAORHF010000000
VERSION     DAORHF010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30492_length_3457_cov_4.267784"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PKJ16_18040"
     CDS             <1..551
                     /locus_tag="PKJ16_18040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV48948.1"
                     /translation="STYASYWIKHYISRFIAKKSRTIRLPIRKGDLFKKVKRVRDELL
                     REFGKEPTRLDIANVLGVEEKAVAEIMEFFQPTMSLEGPLNNDDFSLMQVLGDEKTLS
                     PETMLYRQDLHDELEEALESLMENERKILRMRFGFDDDRVITLKEIGSEFGISAETVR
                     QIENRAIEKIRQKFSHLQDYMD"
     gene            694..1623
                     /locus_tag="PKJ16_18045"
     CDS             694..1623
                     /locus_tag="PKJ16_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HNV48949.1"
                     /translation="MHHHDHGHVHGRVGAPVSRSAKFLALIVFNAVITIAEFVGGVLS
                     GSLALISDAGHNLSDVLALMLGYAGERVSERSPTPRYSFGFKRFEVVVAVVNALSLAG
                     IGIYIVYEALMRYRHPVPIDPGVMLPIAIVGLAGNALSILVINRDRHANLNMRAAFLH
                     LAYDAVSSIAVIAVGITLVFVPRLAVLDLAASCIIVAMIVVSSWGIVRDSLRIFLQGA
                     PKGIDPDAVRRDILAIAGAGSVHGLHIWSVSSAEVFLSCHICAAEGSDPADSDGLIGR
                     VNAMLAERYGITHTTLQVEARALCRLDDGDCCR"
     gene            1720..2625
                     /locus_tag="PKJ16_18050"
     CDS             1720..2625
                     /locus_tag="PKJ16_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48950.1"
                     /translation="MKKNSGFGGRNGVRLILIAMAVLCLVPAFRTASPAADLTVGAVS
                     WYAWWNGFYNDTTKTETDLSNDNKTRLLMYGPVISLGLGDWSISTRFIAGNAEVNEYE
                     AYSYDRESDVKLREKAFKYDSDTTAGYAVSKHVKLFAGVKVMGYQYEKSFWEIRSRGK
                     IVYRHILDYTILAGPGGGIGFSVGLSGDLFLFMNITGIYVTGVSNEKSTAYCTQRFED
                     ENGVDKNGVTVHHADMVQWGGNGDFSLAYYFSGARTIIAVGIRYQHLKCIEYNRSHVV
                     PESMERDGDDRFYGFTVSALRTFEL"
     gene            complement(2634..>3457)
                     /locus_tag="PKJ16_18055"
     CDS             complement(2634..>3457)
                     /locus_tag="PKJ16_18055"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV48951.1"
                     /translation="LTKPFDRRELLARVDTLLTLKNAVYHHNRFERIQRELEIARRIQ
                     NAILPETLPALPGLDIQVAYRPMDSVGGDFYDFHIVDDNRIGILIADVSGHSIPAALI
                     ASMIKIAFYMQSGLADAPDRLMQSIHNTLFDKCESHFITASYSFIDRRRGKLLNANAG
                     HHPCLIHQRSRDRILSVKGKGRIIGLLPSSGYELTETDIAPGDRVVLFTDCILECRTT
                     SGALFGEENFMALVRECAGMSPADFVRHVPDHLSRWSGNEGLFEDDMTIIVVDVK"
BASE COUNT          644 a         1016 c         1105 g          692 t
ORIGIN      
        1 tctcgaccta tgcgtcgtac tggatcaagc actacatcag ccggttcatc gcgaagaaga
       61 gccgcaccat ccggctgccc atcaggaagg gcgacctctt caagaaggtg aagcgggtcc
      121 gcgacgagct tctgcgcgag ttcggcaagg agcccacacg gttggacatc gccaacgtgc
      181 tgggcgtcga ggagaaggcg gtcgccgaga tcatggagtt cttccagccg accatgtcgc
      241 tggagggccc gctcaacaac gacgatttca gcctcatgca ggtgctgggc gacgagaaga
      301 cgctctcgcc ggagacgatg ctctatcgcc aggaccttca cgacgagctc gaggaggcgc
      361 tggagtcgct catggagaac gagcggaaga ttttgcggat gcgcttcggc ttcgacgacg
      421 accgcgtgat caccctgaag gagatcggca gcgagttcgg gatctcggcc gagaccgtgc
      481 ggcagatcga aaaccgcgcg atcgagaaga tccggcagaa attttcgcat ttacaggatt
      541 acatggacta gggtatgctg tgcgggacgc ggtccggtcc cggcgcttcc agtcgtatca
      601 ggtcgcatga tggcgcatgc caatgaaggg cccggtccca tcgcacggcg aatcattccg
      661 aacgccgacg tatgatgtac cctgggggcc cgaatgcacc atcacgatca cggacatgtc
      721 catggccgcg tcggagcgcc ggtttcacgg agcgcgaaat ttctggccct catcgtattc
      781 aacgccgtca tcaccatcgc ggagttcgtg ggcggggtgc tctccgggag cctcgcactc
      841 atctccgacg cggggcacaa cctctccgac gtcctggcgc tcatgctggg ctatgccggc
      901 gagcgcgtct cggagcggtc ccccacgccg cgctattcct tcggcttcaa gcgtttcgag
      961 gtggtcgtcg cggtcgtaaa cgcgctctcc ctggcgggca tcgggatcta tatcgtgtac
     1021 gaggcgttga tgcgataccg ccatcccgtt cccatcgacc ccggcgtgat gctccccatc
     1081 gcgatcgtcg ggctggcggg aaatgcgctg tccatcctgg tgatcaaccg tgaccgccac
     1141 gcgaacctca acatgcgcgc cgccttcctg cacctggcct acgacgctgt gtcctcgatc
     1201 gcggtgatcg cggtggggat tacgctcgtg tttgttccgc gcctggccgt cctcgacctc
     1261 gcggcaagtt gcatcatcgt cgcgatgatc gtcgtgagct cgtgggggat cgtgcgcgat
     1321 tcgttgcgga tcttcctgca gggagcgccg aaggggatcg accccgacgc cgtgcgccgc
     1381 gacattctcg cgattgcggg cgcgggaagc gtccacggcc tgcacatctg gtcggtgagc
     1441 tcggctgagg tgtttctctc gtgccatatc tgcgccgccg agggctctga ccccgcggac
     1501 tccgacggcc tcatcggcag ggtgaacgca atgcttgccg agcgctacgg catcacccac
     1561 accacgctgc aggtcgaggc cagggcgctc tgccgcctcg atgacggcga ctgctgccga
     1621 taggtttcgc gattccgctt tttccctcaa agtttcttga cttttcgcag aaccggccat
     1681 tccttttagt catcgttgca tcgcgtatac ataccagaca tgaaaaagaa cagcggtttt
     1741 ggcgggagga atggcgttcg tttgatcctg atcgcgatgg cagtactgtg ccttgtcccc
     1801 gcgttcagga cggcgtcgcc ggcggcggat ctcaccgtgg gcgccgtctc ctggtacgcg
     1861 tggtggaacg gcttctacaa cgataccacg aagacggaaa cggacctcag caacgacaat
     1921 aaaacccgct tattgatgta cgggcccgtt atctcccttg gccttggcga ctggtcgatt
     1981 tcgaccaggt ttatcgccgg caatgccgag gtgaacgaat atgaggcgta tagctacgat
     2041 cgggagagcg atgtcaaact gcgcgagaag gccttcaagt acgattccga caccacggcg
     2101 ggttacgccg tatcgaagca cgtgaagctg ttcgccggag tcaaggtgat gggatatcag
     2161 tacgaaaaaa gcttttggga gatacggtcc agggggaaaa tcgtatatcg ccatatcctt
     2221 gactacacaa tcctggcggg acccggcggc ggcatcggct tctcggtcgg tctgtcgggc
     2281 gatcttttcc tgttcatgaa cattaccggc atttacgtga cgggcgtctc gaatgaaaaa
     2341 tcaacagcct attgcacgca acggtttgag gacgagaacg gggtggacaa gaacggcgtg
     2401 acggtgcacc atgccgacat ggtgcagtgg ggcgggaacg gcgatttttc gctcgcgtat
     2461 tatttttccg gcgcccggac catcatcgcc gtcggcatcc gctaccagca tctcaagtgc
     2521 atcgaataca atagaagcca cgtggttcca gagtcgatgg agcgcgacgg cgacgaccgg
     2581 ttctacggtt tcacggtctc ggccctgcgc accttcgagc tgtagcgcga ttcctacttc
     2641 acgtccacca cgatgatggt catgtcgtcc tcgaagagcc cctcgtttcc ggaccagcgc
     2701 gagaggtggt ccgggacatg gcgcacgaaa tcggccggcg acatgccggc gcactcgcgc
     2761 acgagcgcca tgaagttctc ctcgccgaag agcgccccgg acgtcgtgcg gcattcgagg
     2821 atgcagtcgg tgaacaggac cacgcggtcg cccggggcga tgtcggtctc ggtgagttcg
     2881 tatccgctcg acgggagcag gccgatgata cgccccttgc ccttgacgga caggatccgg
     2941 tccctgctcc gttgatggat gaggcagggg tgatggcccg cattggcgtt caggagcttt
     3001 ccccggcggc ggtcgatgaa cgaatagctg gcggtgatga agtgcgattc gcacttgtcg
     3061 aagagcgtgt tgtggatgct ctgcatcagc cggtcgggcg cgtcggcgag tcccgactgc
     3121 atgtagaagg cgatcttgat catcgaggcg atgagcgcgg cggggatgct gtggccggac
     3181 acgtccgcga tcaggatgcc gatccgattg tcgtccacga tgtggaaatc gtaaaaatcg
     3241 ccgccgaccg aatccatggg tcggtaggcg acctggatgt ccaggcccgg cagcgccgga
     3301 agggtctcgg gcaggatggc gttctggatg cgtcgggcga tctcgagctc gcgctggatg
     3361 cgctcgaagc ggttgtgatg atacaccgcg tttttcagcg tgagcagcgt gtccacgcgg
     3421 gccaggagct cgcgcctgtc gaagggtttg gtgagat
//
LOCUS       DAORHF010000346         3453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30538_length_3453_cov_4.341377, whole genome shotgun sequence.
ACCESSION   DAORHF010000346 DAORHF010000000
VERSION     DAORHF010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3453
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30538_length_3453_cov_4.341377"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="PKJ16_18060"
     CDS             complement(<1..1332)
                     /locus_tag="PKJ16_18060"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HNV48952.1"
                     /translation="MEQSQKVKKLETIAIVVLAAATLLGGIVFGIITAQVKNFAGIDN
                     LRKFQVSMPTKILDVHGELIAELFQEKRELVAFEDLPNNLIVAFLATEDKEFFSHIGL
                     NPMAIVRAMGRNILAGKIKQGGSTITQQLAKRLFTTGERTFTRKALEAILALQIEKRF
                     TKEEILEMYFNQIYLGHGCYGIATAADFFFNKKAQHLNIDESSVLAALPSAPGRYSPL
                     KNTRNAFRKNWDILSRIVEQGYLTTERAGQIYREFWPRYVEMTKLDSPSRTVYTKSID
                     RAPYFTDYIRQIMITRYGEDAVYNDGLTVYSTLDLRRNTIGEKVLRSGLDQQRAMSDR
                     SHATFDYYHSAVDMSLFSAYNTLRMIFNLPAMQTRADDVTQVKRTLAENIMDELDILS
                     LLVNAGGTSKFIENFREVVLGISTLKVEGALIAVKPQTGYITTMIGGHTFTV"
     gene            complement(1389..1598)
                     /locus_tag="PKJ16_18065"
     CDS             complement(1389..1598)
                     /locus_tag="PKJ16_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48953.1"
                     /translation="MMDERWKNVFMVIGILAVVYVLFWHVLPAVLKAVGWLLGVLFQI
                     AIWGAVIFGAVLLVAYIARMVKNRV"
     gene            complement(1675..2811)
                     /locus_tag="PKJ16_18070"
     CDS             complement(1675..2811)
                     /locus_tag="PKJ16_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HNV48954.1"
                     /translation="MKQRVIFASVLVIAIMLFGLVKINCKSGGSSVFGASGDSPLKSS
                     SEAAKAFEIQKTFRDIYSLYEDRVVFISTEQTVRMRPNPFLDDPFFRQFFGVPNQQRQ
                     MQRKRTGLGTGFVLSEDGYICTNHHVIAGVDKVTVKVGGNSYVAQIVGYDQQTDLALL
                     KVNARLKPVFIGNSDEVKVGDWAIAIGNPFGLDKTFTVGVVSATARRDVDMMGGSQSH
                     IQTDASINPGNSGGPLINIRGEVVGINRMIYSQSGGYMGIGFAIPVNTAKAVLEQLKK
                     HRKIKRGFIGVQVGPVTEEVAASLGLNTVEGALVGDVVEGSPAARGGVQPGDVVMSVN
                     GERIKSYQGLIDAVGAAPVGSTIKMTVWRQKSTVNLFITVVERT"
     gene            3115..>3453
                     /locus_tag="PKJ16_18075"
     CDS             3115..>3453
                     /locus_tag="PKJ16_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HNV48955.1"
                     /translation="MFNKEFEAMPLPKMRELQRERLKWSLTHAYANVPHYKKSFDAAG
                     FNPDTFKTLDDLRRVPFLTKQDMRASYPFGLFAVPLDKVARVHSSSGTTGNATVVGYT
                     TGDVGEFAEVV"
BASE COUNT          640 a         1112 c         1013 g          688 t
ORIGIN      
        1 cactgtgaag gtgtgcccgc cgatcatggt ggtgatatag ccggtctgcg gcttcaccgc
       61 gatcaacgcc ccctcgacct tgagcgtgga gatgcccagc accacctcgc ggaaattttc
      121 gatgaacttg ctggtgccgc cagcgttcac caggagcgag agaatgtcca gctcgtccat
      181 gatgttctcg gcgagcgtcc gcttcacctg ggtcacgtcg tcggccctgg tctgcatggc
      241 gggcaggttg aagatcatcc gcagcgtgtt ataggcgcta aagaggctca tgtccaccgc
      301 gctgtggtaa tagtcgaagg tcgcgtgcga gcggtcggac atcgcgcgct gctggtcgag
      361 cccgctgcgc agcaccttct cgccgatcgt gttgcggcgc aggtcgaggg tcgagtacac
      421 ggtgagcccg tcattgtaca cggcgtcctc gccgtagcgg gtgatcataa tctgccggat
      481 atagtcggtg aaatacggcg cccggtcgat gctcttggtg tagaccgttc gggacgggga
      541 atcgagcttg gtcatctcga catagcgcgg ccagaactcg cggtagatct gcccggcgcg
      601 ctccgtggtg aggtagccct gctccacgat gcgcgagagg atgtcccagt tcttgcggaa
      661 ggcgttccgg gtgttcttga gcggcgagta gcggcccggc gccgagggga gcgcggccag
      721 caccgagctc tcgtcgatat tcaggtgctg cgccttcttg ttaaagaaga aatccgcggc
      781 ggtcgcgata ccgtagcagc catgcccgag gtagatctgg ttgaagtaca tctccaggat
      841 ctcctctttc gtgaatcgct tctcgatctg gagcgccagg atcgcctcga gggccttgcg
      901 ggtgaaggta cgctcgccgg tggtaaacag ccgcttcgcc agctgctggg tgatggtgga
      961 gccgccctgc ttgatcttcc cggccaaaat gtttcgcccc atggcgcgga cgatcgccat
     1021 cggattgagg ccgatgtgac tgaagaattc cttgtcctca gtcgccagga aggcgacgat
     1081 gaggttgttg ggcaggtcct cgaaggcgac cagctcgcgt ttctcctgaa agagctcggc
     1141 gatgagctcg ccgtgcacgt ccaggatctt cgtgggcatg ctcacctgga acttgcgcag
     1201 gttgtcgata cccgcgaagt tcttcacctg ggccgtgatg atgccgaaga cgatcccccc
     1261 gagcagcgtg gccgccgcca ggaccacgat ggcgatcgtt tcgagttttt tcactttctg
     1321 gctctgttcc attgacatcc ctttgcgcta tcgctctgcc gcccgcgggc cgtttccggc
     1381 gcgcgccctt ataccctgtt tttcaccatc cgggcgatat aggcgaccag cagcaccgcg
     1441 ccaaaaatca ccgcccccca gatggcgatc tggaaaagca cccccagcag ccatccgacc
     1501 gccttgagca cggccggcag cacgtgccag aacagcacgt acaccaccgc gagtatgccg
     1561 atgaccatga acacgttttt ccatcgttca tccatcatgc gctccacctc accgacacga
     1621 aaatgcacgc cgcgtacggc gcgataccac tatggcaatc ggcctgcggt ccgtttaggt
     1681 ccgctcgacc acggtaataa acaggttcac ggtgctcttc tggcgccata ccgtcatttt
     1741 gatcgtactg cccaccggag cggcgcccac cgcgtcgatg agcccctgat agctcttgat
     1801 gcgctcgccg ttgacgctca tgaccacgtc cccgggctgc acgccgccgc gcgccgcggg
     1861 gctcccctcg accacatcgc ccaccagcgc gccctcgacg gtgttgagcc ccaggctcgc
     1921 ggcgacctcc tcggtgacgg ggcccacctg cacgccgatg aagccgcgct tgatcttccg
     1981 gtgctttttc agctgctcca gaacggcctt cgcggtgttc acgggaatgg cgaagccgat
     2041 ccccatgtag ccgccgctct gcgaatagat catccggtta atgccaacca cctcgccgcg
     2101 tatgttgatg agcggcccgc cggaattgcc cgggttgatg gaggcgtcgg tctggatatg
     2161 cgactgcgac ccgcccatca tgtccacgtc gcgccgcgca gtggcgctta ccacgcccac
     2221 ggtgaaggtc ttgtccaggc cgaagggatt gccgatcgcg atggcccagt cgcccacctt
     2281 cacctcgtcg gagttcccga tgaacacggg cttgagccgg gcgttcacct tcaggagcgc
     2341 caggtccgtc tgctggtcgt agcccacgat ctgcgcgacg tacgaattgc cgcccacctt
     2401 cacggtcacc ttgtcgacgc ccgcgatcac gtgatggttg gtgcagatgt agccgtcctc
     2461 ggagagcacg aacccggttc ccaggcccgt gcgcttccgc tgcatctggc gttgttggtt
     2521 gggcaccccg aagaattgcc ggaaaaacgg atcgtcgagg aagggattgg gccgcatgcg
     2581 aaccgtctgc tcggtgctga tgaagaccac gcggtcctcg tagaggctat agatgtcgcg
     2641 gaaggtcttc tgtatttcga aggccttcgc cgcctcgctg ctcgacttca ggggcgaatc
     2701 gcccgaggcg ccgaagacgc tggacccgcc gctcttgcag ttgattttca ccaggccgaa
     2761 gagcatgatg gcgatgacga ggacgcttgc gaaaataact ctctgtttca taataccact
     2821 cctggacgct tcgcggcgtc cgatgaccta ccgactggtt cccatacgat tgatgattgc
     2881 aagcggttcg cggcgcgggg gaccggcttc tgtcaacact ccgtcgagca tagcgcacat
     2941 aaaatttacc catagaaaaa atcaataaaa aaaggccaaa ttccgccacg tcgatcgcgc
     3001 cccgccggcc catttttatc gaccgcctac gggaaggttg ttgacaaaaa cgatcgtccc
     3061 gaataagcac gagacgcaac acacccccag catgccgcca tcgaggtaca cgccatgttc
     3121 aacaaagaat tcgaagcaat gccgcttccc aagatgcggg aactgcagcg ggagcgcttg
     3181 aaatggtcgc tcacacacgc ctatgccaat gtcccgcact acaagaagtc cttcgacgcc
     3241 gcggggttca atcccgacac gttcaaaacc cttgacgacc tccgccgcgt cccgttcctc
     3301 accaagcagg acatgcgcgc tagctacccc ttcggcctct tcgccgtccc gctggacaag
     3361 gtcgcccgcg tccactcgtc ctccggcacc acgggcaatg cgaccgtcgt gggctacacc
     3421 acgggcgatg tcggcgagtt cgccgaggtg gtc
//
LOCUS       DAORHF010000347         3452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30552_length_3452_cov_4.421254, whole genome shotgun sequence.
ACCESSION   DAORHF010000347 DAORHF010000000
VERSION     DAORHF010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30552_length_3452_cov_4.421254"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="PKJ16_18080"
     CDS             <1..886
                     /locus_tag="PKJ16_18080"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HNV48956.1"
                     /translation="SAGDHIVATRDIYGGSYRLLTDYLDRFGIEHTFADTSDPREVEH
                     AIRPNTRVLFLESPSNPLLKIIDFEAMAAIARARGCITILDNTFLTPYFFRPLDFGID
                     MSVHSATKFLGGHSDLLAGAVITKSRELGEQVYRVQATTGGVLSPENSWLLLRGIKTL
                     RARMTLQAEGAMRIARWLADQRWATSVYYPGLPEHPGHDILKRQATGFGAVVSFTTDS
                     VARAHAIMKHVQYWTVAVSLGGVESILSYPSRMSHAALPVETRESLGITDTLIRLSVG
                     LEDADDLIEDLARAAEAR"
     gene            927..1370
                     /locus_tag="PKJ16_18085"
     CDS             927..1370
                     /locus_tag="PKJ16_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HNV48957.1"
                     /translation="MKLSTRSRYGVRLMLALGTGEKPGPTLLKDVARREQISEKYLSQ
                     IIIHLKAKGLVHASRGAHGGYELARPAAEITLREIVEPLEGDLCLVDCILRPASCTRS
                     ATCATRDVWGAMNTMILDYLDTITLEDLVRKHRGKLGDAGIDFAI"
     gene            1635..2096
                     /locus_tag="PKJ16_18090"
     CDS             1635..2096
                     /locus_tag="PKJ16_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HNV48958.1"
                     /translation="MIRKILLIPIIASIVFIFGCKGSSDSCCTITPPATGTVSDAPIL
                     TSPGSRIAINNDLSFTYAFDKKPALGTAILKIQVFDKSGKQVSDLAITGDSGMPSMKG
                     HHDSGDVAFQKNRKGDYLMPVQIVMPGDWAVRVKIAKDGAHIYTGLIRFDV"
     gene            2089..3075
                     /locus_tag="PKJ16_18095"
     CDS             2089..3075
                     /locus_tag="PKJ16_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48959.1"
                     /translation="MFNRAVILCACILLITTSAARAQGRLLYLEAQGIAGYSSAEKRM
                     IFYSMRREDAMQKPGLGFDYLERFAGETGDIAMIALQARAAFDDTQPYSTEGQVYNAY
                     LKIKPPFADIWMGHNRPALGISSYLDSHSHLLPTLAMNGFGYDRDWGLGAARQFDWGD
                     IAVSATTGSGMPIYWKGNYLTSGRVSYGVLGRDNYTVGASGAYGQTLDTMGYHLMMPD
                     PMAFAMGGIDYALLWDNLEFSVEGMAGRKGDDPAYAFFFRAGINLMDESRLKLEVQPI
                     YLKTGHEENMQLHATVSYIASGDIKLRAMYAYDRELRDHRVVAQAYWYWRAL"
     gene            3104..>3452
                     /locus_tag="PKJ16_18100"
     CDS             3104..>3452
                     /locus_tag="PKJ16_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48960.1"
                     /translation="MKTILVFLLMIFGSHSAYAAVGCDLNDPDRDVKRLFPGSTGYKT
                     QYVSIDKKGGAALLARIEQRLGDKFQGLFETIDVPYTVYEVYRGTQRIGYIHGVNQKG
                     KFGEATQKWAQRLV"
BASE COUNT          712 a         1054 c         1055 g          631 t
ORIGIN      
        1 ctcggcggga gaccacatcg tcgcgacgcg cgacatctac ggcggctcgt accgcctgct
       61 cacggactac ctcgaccgtt tcggcatcga gcacaccttc gcggacacct ccgacccgcg
      121 cgaggtggag cacgccattc gtccgaacac gcgcgtcctg ttcctcgaaa gcccgtccaa
      181 tcccctgctg aagatcatcg acttcgaggc gatggcggcg atcgcgcggg cgcgcggatg
      241 catcaccatt ctcgacaaca cctttctcac gccctacttc ttccggccgc ttgacttcgg
      301 catcgacatg agcgtccaca gcgcgacgaa gttcctcggc gggcacagcg acctcctcgc
      361 cggcgcggtg atcacgaaat cgcgcgagct cggcgaacag gtgtaccgcg tgcaggcgac
      421 gacgggcggg gtgctctcgc ccgagaactc gtggctgctc ctgcgcggga tcaagaccct
      481 gcgcgcgcgc atgaccctac aggccgaggg cgcgatgcgg atcgcccgct ggctcgcgga
      541 tcaacgttgg gcgacatcag tgtattatcc cggcctgccg gaacatccgg ggcatgacat
      601 cctcaagcgc caggccacgg gcttcggcgc ggtcgtctcg ttcactacgg acagcgtcgc
      661 gcgcgcgcac gcgatcatga agcacgtgca gtattggacc gtcgcggtat cgctgggcgg
      721 ggtggagtcg attctctcgt acccgtcgcg aatgtcgcac gccgcgctcc ccgtcgagac
      781 gcgcgagtcg ctgggcatca ccgacacgct catccgactt tccgtggggc tcgaggacgc
      841 ggacgacctc atcgaggacc tcgcacgggc ggcggaggcc cgatgaggtc gagatcccat
      901 atatcgtcta tcgtgagggt cgcgctatga agctctcaac gcggtcacgc tacggggtca
      961 ggctgatgct ggcgctgggg accggcgaga agccgggacc gacgctgctg aaagacgtcg
     1021 ctcgccgcga gcagatatcg gaaaagtacc tgagccagat catcatccac ctgaaggcca
     1081 agggtctggt gcacgcgtcg cggggcgccc atggcggcta cgaactcgct cggccggccg
     1141 cggagatcac cctgcgcgag atcgtggagc cgctcgaggg cgatttatgc ctggtggact
     1201 gcatcctcag gcccgcgtcc tgcacgcgtt ccgcgacatg tgcaacccgg gacgtatggg
     1261 gggcgatgaa cacgatgatc ctggattatc tcgacaccat tacgctcgag gacctggtgc
     1321 gaaaacaccg cggcaagctg ggcgatgcag ggattgattt tgcgatatag gctccttagc
     1381 caggccgtcc agtatatggc acattctcat cgatcttcat gataatgagt acgtcaggga
     1441 aaaagtgttg catgttctga aggtctcaac actccaacgc ggggtcagag ccccttgacg
     1501 cggggtcaga gccccaacac aattcaccag cttgttcgca tttatggtac tttagaataa
     1561 actttattct taaaacgacc agacatgatg gtatactaag tatcagactt ttattaaaaa
     1621 caaggagcac gccaatgatt cgaaaaatac tgcttatacc gataatcgca tcgatagtgt
     1681 tcatttttgg ctgcaaggga tcgtccgatt cctgctgcac catcacaccg ccggcgaccg
     1741 gaaccgtgtc cgatgcgccc atactcacct cgcccgggag caggatcgcc atcaacaacg
     1801 acctgtcttt cacatacgcc ttcgataaaa aaccggcgct ggggaccgct atcctcaaga
     1861 tccaggtgtt cgacaagtcg gggaaacagg tatcggacct cgccatcacc ggcgactcgg
     1921 ggatgccgtc gatgaaggga caccacgaca gcggcgatgt cgcctttcag aaaaaccgga
     1981 agggcgatta cctcatgccg gtgcagatcg tgatgccagg cgactgggcc gtccgggtga
     2041 agatcgccaa ggacggcgct cacatctata cggggttgat acgattcgat gtttaacagg
     2101 gcggtcatcc tgtgcgcgtg catactgctg ataacgacat cggcggcgcg cgcccaggga
     2161 cgactgctct atctggaggc gcagggaatc gccggctact cgagcgcgga gaagcgcatg
     2221 atcttctact cgatgcgccg ggaggacgcg atgcaaaaac ccggcctggg attcgactac
     2281 ctcgaacggt tcgccgggga gaccggcgat atcgccatga tcgccctcca ggcgcgcgcc
     2341 gccttcgacg acacgcagcc ctacagcacc gagggacagg tctacaacgc ctatctgaag
     2401 atcaagccgc cgttcgccga tatctggatg gggcacaacc gccccgcgct gggcatctcg
     2461 tcgtatctgg acagccacag ccatctcctg ccgacgcttg cgatgaacgg tttcggctac
     2521 gaccgcgatt gggggctcgg cgccgcgcgg cagttcgact ggggcgatat cgccgtctcg
     2581 gcgaccaccg gctccgggat gcccatctac tggaagggga actatctcac atccggcaga
     2641 gtatcgtacg gcgttttggg gcgcgataac tataccgtgg gcgcaagcgg cgcctacggc
     2701 cagacgctcg acacgatggg gtatcatctc atgatgcccg atccgatggc cttcgcgatg
     2761 ggcggcatcg actacgcgct cctgtgggac aatctcgaat tcagcgtgga gggcatggcg
     2821 ggccggaagg gggacgaccc cgcgtatgcg ttttttttcc gcgcaggcat caaccttatg
     2881 gacgagagcc gtctcaagct tgaggtccag ccgatatacc tgaagaccgg ccacgaggag
     2941 aacatgcagc tgcacgcgac ggtttcgtac atcgccagcg gcgacatcaa actgcgcgcg
     3001 atgtatgcat acgaccgcga actgcgggat catcgcgtcg tcgcgcaggc gtactggtac
     3061 tggagggcgt tatgattgtg attacagtta caggaggatt ccaatgaaaa ccatactcgt
     3121 atttctgttg atgattttcg gctcgcacag cgcatatgcg gccgtcggct gcgatctgaa
     3181 cgatccggac cgggacgtga agcggctctt cccgggatcg accggctaca agacgcagta
     3241 tgtgtccatc gacaaaaagg gcggcgcggc gctcctggcg cggatcgaac agcgactggg
     3301 agacaaattc caggggctct tcgagaccat cgacgtcccc tacaccgtgt acgaggtcta
     3361 ccgcgggacg cagcggatcg gctacatcca cggcgtaaac cagaagggga agttcggcga
     3421 ggcgacacag aagtgggccc aacgcctggt gc
//
LOCUS       DAORHF010000348         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_30964_length_3422_cov_4.537868, whole genome shotgun sequence.
ACCESSION   DAORHF010000348 DAORHF010000000
VERSION     DAORHF010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_3422_cov_4.537868"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PKJ16_18105"
     CDS             complement(<1..477)
                     /locus_tag="PKJ16_18105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV48961.1"
                     /translation="MVASRPARGRMEELFTGAGIALSRAQLDRFWAFHLLIEKHNDEL
                     DLTRIRRFDDIVIKHFIDCIHVTALCELAGPLVDIGTGAGFPGIPLKIVRPELEIVLA
                     DTRSKRTDFLALVVAELGLEGVHIYPHKVTDLSFFEANGVITRALESADDTLNRVHH"
     gene            complement(471..1382)
                     /locus_tag="PKJ16_18110"
     CDS             complement(471..1382)
                     /locus_tag="PKJ16_18110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV48962.1"
                     /translation="MKMRTIPSATLVLCIALLCAACSKSGMGAHHDFFATEDDYVQYV
                     EQNVRFDGLELAIVDIQRGENFWKIAKRHNVNIDTLLGANPYWESLVASLDQRIIVPS
                     RKGVLHFVNSRSEIRDVAKAYQADESSVLVQRLPVMDGILRRFTSRSPIAVFVLNARP
                     RAINMTEALASQFNLREMFRSPLGGRYSSFFGGRIHPIFREHRFHNGLDIAARHGTPV
                     GAAAGGVVTSTGWMGGYGNTVIISHPKGFRTLYGHLSRISVRAGQQVGAGRLIGRVGS
                     TGWSTGPHLHFTMWHNGKLVNPMKVLW"
     gene            complement(1571..1774)
                     /locus_tag="PKJ16_18115"
     CDS             complement(1571..1774)
                     /locus_tag="PKJ16_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HNV48963.1"
                     /translation="MPKGVVKWFNSKKGFGFIKKEDGSDIFVHFTGIGGDGFKSLNEG
                     DNVEFQIENSDKGPRAIDVRVAQ"
     gene            2021..3325
                     /locus_tag="PKJ16_18120"
     CDS             2021..3325
                     /locus_tag="PKJ16_18120"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV48964.1"
                     /translation="MSIYELFVGLRYLKAKKSQGFISFNTVLSIVTVFIGVFMLIVVI
                     SVMNGFQSQIKDKILDVDSHITVISGFGDENENITDYRKLVERLRSVNGVRAVTPYIQ
                     GQGLFRYKTRITPILLRAMGDTTSMPDEVKKFVTKGAREFKSASDVFVGAEMAANYGI
                     KVGDAIEVIVPKGTIKITTGLMPGIGTFRVAGFFKSGYYEFDTKLVVMSLPAAQQLYE
                     IGDVVWGIGLKIDDIYRMDYMASMVQTRLGSFRYHTLTAEERNQNLFYALKLEKLIMT
                     VILFLVIIAAGFTIMGTLVMVVMEKRKAIGVLKSMGARPISIMIIFVLEGFLIGVLGA
                     TLGSVFGIAASLNLEAIIKWVESAINGVMAFVYGVFNLGAYFPMSLVPKNVYYIDTIP
                     TEIKPEFIVFIAIFAVFLSTIAAIFPSWHASRLKPVETIRYE"
BASE COUNT          644 a         1016 c         1077 g          685 t
ORIGIN      
        1 gtgatggacg cggttcaggg tatcatcagc ggattccagc gcgcgggtaa tcacgccgtt
       61 cgcctcgaaa aacgagaggt ccgtcacctt gtgcgggtag atgtgaacgc cctcgaggcc
      121 cagctcggcg acgacgagcg cgagaaaatc ggtccgcttg ctccgcgtgt cggcaaggac
      181 aatctcgagc tcgggccgga cgatcttgag cggaatgccc gggaaaccgg ccccggtccc
      241 gatgtccacg agcggtcccg caagctcgca cagcgcggtc acgtgtatgc agtcgatgaa
      301 gtgcttgatg acgatgtcgt cgaagcggcg gatgcgcgtg aggtcgagct cgtcgttatg
      361 cttttcgatg aggaggtgga acgcccagaa ccggtcgagc tgggcgcgtg aaagcgcgat
      421 cccggcgccg gtgaagagct cctccatccg gccgcgcgcg gggcgcgacg ctaccacagc
      481 accttcatgg ggttcaccag tttgccgttg tgccacatgg tgaagtgcag gtgcggcccc
      541 gtggaccagc cggtcgatcc cacgcggccg atgagccgcc cggcgcccac ctgctgcccg
      601 gcgcgcacgc tgatgcgcga gaggtgcccg tagagcgtgc ggaacccctt cgggtgcgag
      661 atgatcacgg tgttcccgta tccccccatc catccggtgg aggtgaccac gccgccggcg
      721 gctgcgccca ccggcgtgcc gtgccgcgcc gcgatgtcca ggccgttgtg gaagcggtgc
      781 tctcggaaga tcgggtggat gcgcccgccg aagaacgaac tgtaccggcc gccgagcggc
      841 gatcggaaca tctcgcgcag gttgaactgt gaggcaaggg cctccgtcat gttgatggcg
      901 cgcggccgcg cgttcagtac gaagacggcg atgggcgagc ggctcgtgaa gcggcgcagg
      961 atcccgtcca tgacgggcag ccgctggacg aggacgctcg attcatccgc ctgatacgcc
     1021 ttcgcgacgt cccggatctc cgagcgcgag ttcacgaagt gcagcacccc cttgcgcgac
     1081 ggaacgatga tgcgctggtc gagcgaggcg accagcgact cccagtaggg gttggccccc
     1141 aggagcgtgt cgatgttcac gttgtggcgc ttggcgatct tccagaagtt ctcgccgcgt
     1201 tgtatgtcga cgatcgccag ctcgagcccg tcgaatcgga cgttttgttc gacgtattgg
     1261 acatagtcgt cctcggtcgc gaagaagtcg tggtgggcgc ccatgccgga tttcgagcac
     1321 gccgcgcaga ggagcgcaat gcacaggacg agcgttgcgg acggaatggt tctcattttc
     1381 atgcggtttc ccgttgctgt ttcgcgagag ggtttggcgt ccgggtcccg cttgaccgtg
     1441 ttccgtttgc gcgggaggga ctgcccttca acaatactat cggccccatt tgtcaagagc
     1501 attatcgggg cggaggataa aaaagggcgc ccgctggcgc cctcgtgtcg gtcatgcggc
     1561 cgatgtgaaa ttactgcgcc actcgaacat ctatcgcgcg cggtcccttg tcgctgtttt
     1621 cgatctgaaa ctcgacgttg tcgccctcgt tgagggactt aaagccatcg ccgccgattc
     1681 cggtgaagtg gacgaagatg tcgctgccgt cctccttctt tatgaacccg aacccctttt
     1741 tgctgttaaa ccacttaacc actcccttcg gcatgatgca gactcctgaa ccttaagatg
     1801 tcgactggtt tcagctgcag cgctccagcg ggcctagcac tgctaaccga gatccggggc
     1861 gaccccatag ccaaaactac ggtttcattt taccgacgtt catcgttttt cgtcaattaa
     1921 aaaatgggtg cgtcgggctg tgccgggtaa tatgtattga caaaatagcc gcgagggcta
     1981 cggttccgtt gcgatactga aatcatgggg attgtgcgat atgagcatct atgaactgtt
     2041 cgtgggcctc cgctatctca aggccaagaa gagccagggg ttcatatcct tcaacacggt
     2101 cctgtcgatc gtcacggtct tcatcggcgt gttcatgctc atcgtggtca tctcagtgat
     2161 gaacggcttc cagagccaga tcaaagacaa gatcctggac gtcgattcgc acatcaccgt
     2221 catcagcggt ttcggcgacg agaacgaaaa catcaccgac taccggaagc tcgtcgagcg
     2281 gctgcggagc gtcaacgggg tccgtgcggt cacgccgtac attcagggcc aggggttgtt
     2341 tcggtacaaa acccggatca cccccatcct gttgcgggcc atgggcgaca ccacgtccat
     2401 gcccgacgag gtgaagaagt tcgtgaccaa gggcgcgcgc gagttcaagt cggcaagcga
     2461 cgtgttcgtg ggcgccgaga tggccgcaaa ctacggcatc aaggtcggcg acgcgatcga
     2521 ggtgatcgtc cccaagggca ccatcaagat cacgaccggt ctcatgccgg gaatcggcac
     2581 cttccgggtt gcgggcttct tcaagtccgg atactacgag ttcgacacca agctggtggt
     2641 gatgtcgctc ccggcggcgc agcaactcta cgagatcggc gacgtggtgt gggggatcgg
     2701 gcttaagatc gacgacatct accgcatgga ctacatggcc tcgatggtgc agacgcgcct
     2761 ggggtccttc cgctatcaca ccctcaccgc ggaggagcgc aaccagaacc tcttctacgc
     2821 gctcaagctg gagaagctca tcatgacggt gatccttttc ctggtgatca tcgccgccgg
     2881 cttcaccatc atggggacgc tggtgatggt ggtgatggag aagcgcaagg cgatcggcgt
     2941 gctcaagtcg atgggcgcgc gccccatctc catcatgatc atcttcgtgc tcgagggctt
     3001 cctcatcggc gtgctggggg cgacgctggg ttccgtgttc ggcatcgccg cgtcgctcaa
     3061 tctcgaagcc atcatcaagt gggtcgagtc ggccatcaac ggcgtgatgg cgttcgtgta
     3121 cggcgtgttc aatttggggg cctacttccc gatgtcgctg gtgccgaaga acgtgtacta
     3181 tatcgacacc atccccacgg agatcaagcc ggagttcatc gtgttcatcg cgatcttcgc
     3241 ggtgttcctc tcgaccatcg cggcgatctt tccgtcgtgg cacgcctcgc gcctcaagcc
     3301 ggttgaaacg atccgatacg aataatacgg gggcacagac gagtcatggc acaagacctc
     3361 atcatacagg ccgcgaacat cgaaaagtcc tacggcgccg ggcgcagcgc ggtgcgggtg
     3421 ct
//
LOCUS       DAORHF010000349         3399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31284_length_3399_cov_4.131280, whole genome shotgun sequence.
ACCESSION   DAORHF010000349 DAORHF010000000
VERSION     DAORHF010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31284_length_3399_cov_4.131280"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PKJ16_18125"
     CDS             complement(<1..298)
                     /locus_tag="PKJ16_18125"
                     /inference="COORDINATES: protein motif:HMM:NF023834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HNV48965.1"
                     /translation="MALNPLIDSRDVRFVLFEMLELEKFGKDYSHYADFDRDTYEEFL
                     NLAERIAVEKIYPASKEGDKEGCTYNPDTKEVKIPKAYKPAIDAFYEAGFLGTID"
     gene            564..1508
                     /locus_tag="PKJ16_18130"
     CDS             564..1508
                     /locus_tag="PKJ16_18130"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HNV48966.1"
                     /translation="MSDYKYWMALEQSSGMGPAHCATVAAALESRSLSLADIFMLDAA
                     DLKAEFPFNETIVKGLAGARAILDRVEQDYLRLMEGGVETVLFFEPSYPPRLRDRIAG
                     AFPPILYAHGNRALLKDPAIAILGDKDVSDKGGVIAHHASAELVRRRFAIISGLARGV
                     GQIAHRAAAESGGATIAVLPYGMFHLAIPDLLHEVYDPDRFLILSPFYPTTPFTTFNA
                     FTRNRMICALSDAVYIVESPVEGGIFEAGKSARKIGVPLFTTEYAEYPKSAAGNKTLI
                     DELGAIPVRGRLKDNVLQPNLDRLIGAAKFPSSAPPAK"
     gene            1615..>3399
                     /locus_tag="PKJ16_18135"
     CDS             1615..>3399
                     /locus_tag="PKJ16_18135"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HNV48967.1"
                     /translation="MHLSLKKKILIASLVVNLCLSSLLGIALYWFAGDQYYEAFLESK
                     LSLARSLAMSIDGSKHATFTSLRAMNDPDYQRTLRYLNSVRTNEKYVSYLFTLHYDPA
                     RDRVSYIVDSDILVRDTVWITTEHFGLAMTIDASDRISLKYNEEERTKDFPVKVGEKT
                     VPLQIRNESEIWIDNKRILTVAARNPLTIETPAGTLSRDHRERYVGAEIGGTAMQVYC
                     TFSAAGESQSIPGEQYMESKEVVERCKSVIRGQAHTIVRRGQQTSIYGMNTATVYGVI
                     KDARGVANGLVVFELFVREVSNFKLAMVRIVIIAAAVIFLVTLLLSNMLSRSLVTPIR
                     RLTEGANRIREGDLDHAVEMERTDELGVLAESFNNMVSRLKTTQRELAATNAELTRAN
                     LLKDQFLANTSHELKTPLNGIIGIADSLIDGAAGSVNSHQAENLRLISLSGKRLFHLV
                     NDILDFSKLKNRDISIDPKPVDIRQIAEIVIILCTHLVGKKPLRIVNQIEPHLPPVLG
                     DEDRLQQIMYNLVGNAIKFSDAGTIAITAAIAGDSVDVTVSDTGIGIPADRFEDIFKY
                     FEQLDAGPARRYGGTGLGLALTRQLVELH"
BASE COUNT          669 a         1116 c         1001 g          613 t
ORIGIN      
        1 aatcgatggt gccgaggaag ccggcttcgt agaacgcgtc gatggcgggc ttgtaggcct
       61 tgggaatttt cacttccttg gtgtcgggat tgtaggtgca gccttccttg tcgccttcct
      121 tgctggccgg atagatcttc tccaccgcga tccgctcggc gaggttcagg aactcctcgt
      181 aggtgtcacg gtcgaaatcg gcgtagtgcg agtagtcctt gccgaatttt tcaagctcta
      241 gcatctcgaa cagcacaaac cgaacgtccc tcgaatcaat taacggattt aacgccatga
      301 tacaacctcc tcgatacaat aattaattat tcatcgcgcg tgagacagaa ataacgttga
      361 caaatccctc caccgccgat ggcattccgt caccgatggg gcgcatgctc gaagcgcgcc
      421 ccgcacacga caggggtttt tcgaatcaag cctattttcg ggggtgtatc tgtcaaccgg
      481 ctttttgcgg tttttcggcc caagtattga aaaaagccgg cgcaatcccc gcatctccgc
      541 gtacgatcgt tcgatgggag ccgatgtccg attacaaata ctggatggcg ctggaacagt
      601 ccagcgggat gggaccggcg cattgtgcga ccgtcgccgc cgcgctcgag tcgcgctccc
      661 tctcccttgc cgacatattc atgctggatg cggcggacct gaaggccgag ttccccttca
      721 acgagacgat cgtcaaggga cttgccggcg cccgcgccat ccttgatcgc gtcgagcagg
      781 attatcttcg cctcatggag ggcggcgtcg agaccgttct attcttcgag ccgtcatacc
      841 cgccccggct tcgcgaccga atcgcgggcg ccttcccccc catcctgtat gcccacggga
      901 accgcgccct cctgaaggat ccggccatcg ccatactcgg cgacaaggac gtgagcgaca
      961 agggaggggt gatcgcgcat cacgcatccg ccgagctggt gcgccgccgg ttcgcgatca
     1021 tcagcggcct ggcgcgcggc gtgggccaga tcgcgcaccg cgcggccgcc gagagcgggg
     1081 gcgcgaccat cgcggtgctc ccctacggca tgtttcatct ggccatcccg gaccttctcc
     1141 atgaggtgta cgatccggac cggttcctga tcctctcgcc gttctatccg acgacgccct
     1201 tcacgacctt caatgccttc acccgcaacc gcatgatctg cgcgctgtcg gacgcggtgt
     1261 acatcgtgga atcgcccgtc gagggcggca tcttcgaggc cggcaagtcg gcgcggaaga
     1321 tcggagtgcc gctgttcacc accgaatacg cggagtatcc gaaaagcgcc gcggggaaca
     1381 aaaccctcat cgacgagctg ggcgcgatcc ccgtacgcgg tcgcctgaag gacaacgtgc
     1441 tgcagcccaa cctggaccgg ctcatcggcg cggcgaaatt cccgtcatcg gcgccgccgg
     1501 cgaaatgacg ggacgcgaca cgggccccgc gcgccccgca ttcgcgcagg gcataatgta
     1561 tttgattttt ttcacgccat caccgagcat gcgttccggc ggatatttgg caccatgcac
     1621 ctctccctga aaaagaagat cctgatcgcc tccctggtgg taaacctgtg cctgtcctcg
     1681 ctgctgggga tcgcgctcta ctggttcgcc ggcgaccagt attacgaggc ctttctcgaa
     1741 agcaagctct cgttggcgcg ttccctcgcc atgtccatcg acggatcgaa gcacgccacg
     1801 ttcacctcgc tgcgcgcgat gaacgacccc gactaccagc gcaccctgcg atatctcaac
     1861 agcgtccgga cgaacgagaa atacgtaagt tacctgttca cgctccacta cgatcccgcg
     1921 cgcgaccgcg tctcctacat cgtcgacagc gacattctgg tccgcgacac cgtctggatc
     1981 accacggagc acttcggcct ggcgatgacc atcgacgcga gcgaccgcat cagcctgaaa
     2041 tacaacgagg aggagcgcac gaaggacttc ccggtgaagg tgggtgagaa gaccgtgccg
     2101 ctgcagatca ggaacgagag cgagatctgg atcgacaaca agcggatcct gaccgtcgcg
     2161 gcgcgcaatc cgctgaccat cgagaccccc gccgggacgc tcagcaggga tcatcgggag
     2221 cgctacgtgg gcgccgagat cggcggcacg gccatgcagg tctactgcac cttctcggcg
     2281 gcgggcgaat cgcagagcat cccgggcgag cagtacatgg agtcgaagga ggtggtggag
     2341 cgctgcaaga gcgtcatcag gggacaggcg cacaccatcg tccggcgcgg ccagcagacg
     2401 agcatctacg gcatgaacac cgccaccgtg tacggggtga tcaaggacgc gcggggcgtc
     2461 gccaacgggc tggtggtctt cgagctcttc gtgcgcgagg tgtcgaactt caagctcgcc
     2521 atggtgcgga tcgtcatcat cgccgctgcg gtgatctttc tcgtgaccct gctgctgtcg
     2581 aacatgctgt cacgctcgct ggtcaccccc attcggcggc tcacggaggg cgccaaccgg
     2641 atccgagagg gcgacctcga tcacgcggtc gaaatggagc gcaccgacga gctgggcgtc
     2701 ctggccgaaa gcttcaacaa catggtctcg cggctcaaga ccacccagcg cgagctcgcg
     2761 gcgacgaacg ccgagctgac gcgggcgaac ctattgaaag accagttcct cgccaacacc
     2821 tcccacgagc tcaagacgcc gctaaacggc atcatcggca tcgccgattc gctcatcgac
     2881 ggggccgcgg ggtccgttaa cagccatcag gctgagaacc tgcgcctcat ctcgctgtcg
     2941 ggaaagcgcc tcttccatct cgtgaacgac atcctcgact tctccaagct gaagaaccgc
     3001 gacatcagca tcgaccccaa gccggtggac atccgccaga tcgccgagat cgtcatcatc
     3061 ctgtgcacgc acctcgtggg gaaaaaaccg ctgcgcatcg tcaaccagat cgagccgcac
     3121 ctgccgccgg tgctcgggga cgaggaccgc ctgcagcaga tcatgtacaa cctcgtcgga
     3181 aacgcaatca agttctcgga cgccgggacc atcgccatca ccgcggccat cgcgggggac
     3241 agcgtggatg taaccgtctc cgatacgggc atcggcatcc ccgccgatcg cttcgaggac
     3301 atcttcaaat acttcgagca gctcgatgcg gggcccgcgc gccgatacgg cgggacgggc
     3361 ctggggcttg ccctcacccg gcaactggtg gagctccac
//
LOCUS       DAORHF010000350         3386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31448_length_3386_cov_4.949865, whole genome shotgun sequence.
ACCESSION   DAORHF010000350 DAORHF010000000
VERSION     DAORHF010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31448_length_3386_cov_4.949865"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PKJ16_18140"
     CDS             <1..294
                     /locus_tag="PKJ16_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNV48968.1"
                     /translation="EDLARFTGVGDDEIFCQVIDYGVDYPSGNPKSIAEVSYAELKTG
                     RVTVHGKEIPAAPLSSYPLAKRIAGILRDWIKSGQFTLGVPQIPLPTCPNDMK"
     gene            294..1037
                     /locus_tag="PKJ16_18145"
     CDS             294..1037
                     /locus_tag="PKJ16_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HNV48969.1"
                     /translation="MRHTERAYRDFSDSDRWRAFHVRVETTDLYIRAAADYSSRVRDL
                     VSRLRGEILAHIAQHRDFLTALAPIARIDTASYIVNSMLDASEAAGVGPMAAVAGAIA
                     AAVGSDLAADSNEVIVENGGDIWLRLASPALVDVYAPGTPFAGRLALRIDPAKTPLGI
                     CTSSGRVGPSLSFGRADAATVLATDAALADAVATETGNRARRPEDAGAALEYALGVPG
                     VIGALVVIRDTLAVRGDIELVDPSDDGAR"
     gene            complement(1034..2275)
                     /locus_tag="PKJ16_18150"
     CDS             complement(1034..2275)
                     /locus_tag="PKJ16_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48970.1"
                     /translation="MIEKRILVAEDETASADLIREMLAASGYAVTLCEHGAAALEAYR
                     ERPFPVVVTDIEMPVMDGNELVTRLKKIDEDQIVIVLTVHSEPDVIIDIMRKGVYDYV
                     IKPARSAEMLYKLERAFEAAESRRMRRTIEREKVVRLENQLEWFKWSDKIIQRDYDRV
                     DRTLFASLHMSFNQGAGFGALLTLISLISTSAKREGDTYSIDANLFNLINENAKMAER
                     ALNVFSDINNIICNELKREAVSLADLYAFIQLVIRERGEAAAIKGHTIRLSEMKSWSG
                     SDTVLIDSGNLYKAIAELLVNALKFSEPRSDIFVILDYHRTESVTISIINRPGANERG
                     IEGIPAEYENLVFEPFFRLVSSTYEQYNTLDYGLGLTLVEKILKRHDGSVTVSNIMDH
                     TDITRDPVVKVNFTIALPIAR"
     gene            complement(2272..>3386)
                     /locus_tag="PKJ16_18155"
     CDS             complement(2272..>3386)
                     /locus_tag="PKJ16_18155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV48971.1"
                     /translation="LLSNAIKYTDPGRRVGLTVRRGNARVVIEVWDEGRGISADVYDA
                     IFQPFVQVRDFNASKPMGTGLGLSITKRLVELHGGTIKVQSAIGQGSRFTIIMPNVPS
                     AQERSAESPVRGSLNDAQDRFAGASVLVVEDNELNSILMRTLLEEFGISVQCVPTGED
                     AVSLLAEHRVDLVFMDIDLPGMSGVEAAHRLREQDADCPPIVALTAYSMKGDRERYLA
                     EGMDGYISKPIEIAGIIAVLDQFLRREGAREDVARVDGAPPMRYDFGAVAREIGIDTA
                     VFKGIVKKFFDSVSDEYLRDLREAVARGDHDRIHRAAHRFKGSAANLRFGTLVEILRT
                     IDEHARRADDVDYASLCDRVDDELEVLRRNVHGGLL"
BASE COUNT          632 a         1086 c         1001 g          667 t
ORIGIN      
        1 gaggacctcg cgcgcttcac cggcgtgggg gatgatgaga tcttctgcca ggtgatcgac
       61 tacggcgtag attatccgtc tggaaacccg aagagcatcg ccgaggtgag ctacgccgag
      121 ctgaagaccg gccgcgtgac cgtacacggc aaggaaatcc ccgcggcgcc gctgtcgagc
      181 tatccgctcg cgaagcgcat cgccggaatc ctccgcgact ggatcaagag cggccagttc
      241 accctgggcg tgccgcagat tcccctgccg acctgcccga acgacatgaa gtgatgcgcc
      301 acaccgagcg cgcctatcgg gacttctccg attcggaccg ctggagggcc tttcacgtac
      361 gcgtggaaac gaccgatctc tacatccgcg cggccgccga ttactcttcg cgcgtacgcg
      421 atcttgtctc gcgtcttcgc ggggagatac tcgcgcatat cgcgcagcat cgcgacttcc
      481 tgaccgccct tgcgccgatc gcgcgcatcg acaccgcctc ttatattgtg aattcaatgc
      541 ttgatgcgtc ggaggccgcc ggggtggggc cgatggccgc cgtcgcgggc gccatcgccg
      601 ccgcggtggg gagcgatctc gcggcggact cgaacgaggt gatcgtagaa aacggcggcg
      661 atatctggct tcggctcgcc tcgcccgcgc tggttgatgt ctacgccccg ggaaccccct
      721 tcgcggggcg cctcgcgctt cggatcgatc ctgcgaaaac gccgcttggg atttgcacct
      781 catcggggag agtgggcccc tcgctcagct tcggccgcgc cgacgccgcg acggtcctcg
      841 cgactgacgc cgcactggcc gacgccgtgg cgaccgagac cggcaaccgc gcgcgtcgac
      901 ccgaagacgc cggagccgcg ctggagtacg cgctcggcgt acccggcgtc attggcgcgc
      961 tcgtcgtcat ccgcgacacc ctcgccgtgc ggggcgacat cgagctcgtc gatccgtcgg
     1021 atgacggcgc gcgctagcgc gcgatcggca atgctatggt gaaattcact ttgacgaccg
     1081 gatcgcgcgt gatgtcggtg tggtccataa tattcgaaac ggtcacggac ccatcatgcc
     1141 gcttcagtat cttttcgacc agcgtgaggc caagcccgta atccagcgta ttgtactgtt
     1201 cataggtcga gctcactaga cggaagaagg gctcgaacac gaggttctcg tattccgcgg
     1261 ggatgccttc gataccccgc tcgttcgctc cgggacggtt gatgatggag atggtcacgg
     1321 actccgtgcg gtgataatca agaataacga agatgtccga tcgcggctcg gagaacttga
     1381 gcgcattgac cagcagctcg gcgatcgcct tgtacaaatt gccgctgtcg atgagcaccg
     1441 tgtcgcttcc cgaccacgat ttcatttcgc tgagccgaat cgtgtggccc ttgatcgcgg
     1501 cggcctcccc gcgctctctg atgacgagct gaatgaaggc gtacagatcc gcgagcgaaa
     1561 cggcctcccg tttcagctcg ttgcagatga tgttgttgat gtcggaaaac acgttgaggg
     1621 cgcgctccgc catcttcgcg ttttcgttga tgagattaaa caggttcgcg tcgatgctgt
     1681 aggtgtcccc ctccctcttt gccgatgtcg atatcaggct gatgagcgtg aggagcgcgc
     1741 cgaatcccgc cccctggttg aagctcatgt ggaggctggc gaagagggtg cggtccaccc
     1801 ggtcgtaatc gcgctggatg atcttgtcgc tccacttgaa ccactcgagc tggttctcga
     1861 gccgcaccac cttctcgcgc tcgatcgtgc gccgcatacg cctgctttcg gccgcctcga
     1921 aggcccgctc cagcttgtag agcatctcgg cggatcgcgc cggcttgatg acgtaatcgt
     1981 acacgccctt gcgcataatg tcgatgataa cgtcgggctc gctgtgaacg gtgagcacga
     2041 tcacgatctg gtcctcgtcg atcttcttga ggcgggtgac cagctcatta ccgtccatca
     2101 ccggcatctc gatatcggtc acgaccaccg ggaagggacg ctcgcgatag gcctcgagtg
     2161 cggccgcccc gtgttcgcac agggtcaccg catagccgct ggcggcgagc atctcgcgaa
     2221 tgaggtcggc gctcgccgtt tcgtcctcgg caaccagaat tcgcttttcg atcataacaa
     2281 ccctccatgc acattacggc gcagtacttc gagttcgtcg tcaacgcggt cgcatagcga
     2341 cgcgtaatcg acatcgtccg cccgccgggc atgctcgtca atcgtgcgca aaatttcaac
     2401 aagagttcca aatcgcagat tggccgccga ccccttgaac cgatgcgcgg cccggtggat
     2461 gcgatcatgg tcgccgcgcg ccacagcttc gcgtaaatcg cgaagatact cgtccgagac
     2521 cgaatcgaaa aactttttca cgataccctt gaacaccgcg gtgtcgatgc cgatctcacg
     2581 cgcgaccgca ccgaaatcgt accgcatggg cggtgcgccg tcgacgcgcg cgacatcttc
     2641 ccgggctccc tcacggcgta aaaactggtc aaggaccgca atgatcccgg cgatctcgat
     2701 cggtttggag atatatccat ccatgccctc cgccaggtat cgttcacggt cgcccttcat
     2761 cgagtaggcc gtgagcgcga caatcggcgg gcagtccgcg tcctgttcgc gcaagcggtg
     2821 ggccgcctct acgccgctca tgccgggaag atcgatgtcc atgaacacta aatcaacgcg
     2881 atgttcggcg aggagcgata cggcatcctc gcccgttggc acgcactgga cggaaatccc
     2941 gaactcctca aggagcgtac gcatgaggat gctattgagt tcattgtcct cgactacgag
     3001 caccgatgca ccggcaaatc ggtcctgcgc gtcgttgaga gatccccgca ccggcgactc
     3061 ggcgcttctc tcctgcgcag agggaacgtt gggcatgata atggtgaaac gactcccctg
     3121 gcctatcgca ctctggactt ttattgtgcc gccatgaagc tccacgaggc gcttggtgat
     3181 ggaaagaccc agtcccgtcc ccatgggctt cgatgcattg aaatcgcgca cctgtacgaa
     3241 cggctggaaa atcgcatcat acacgtcggc ggaaataccg cgtccctcgt cccacacctc
     3301 gatgaccacc cgggcatttc cccgacgcac cgtaaggcca acacgccgcc ccgggtcggt
     3361 atacttaatc gcattcgaga gaagat
//
LOCUS       DAORHF010000351         3384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31478_length_3384_cov_4.910183, whole genome shotgun sequence.
ACCESSION   DAORHF010000351 DAORHF010000000
VERSION     DAORHF010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31478_length_3384_cov_4.910183"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PKJ16_18160"
     CDS             complement(<1..1663)
                     /locus_tag="PKJ16_18160"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HNV48972.1"
                     /translation="MALCLLVWTACAGCGPLSSKEKRPVAVKGVIDLRDWDFERDGAI
                     NLNGEWEFCWDRELAPADFTRPENTRGCSYITVPGLWEKQNLNGNPLPGEGRATYRLK
                     ILRGPDNRAKTMTIHRIYSAYRLFVNGVPAGGRGSMSGSPPEKREDFIYIHNTYKASF
                     PLRPGVNEIVLQVVNRDHDCGGIDRALTLNDRDADARTVCILHAIDMMIIGLALFTAI
                     YNMLLYGFRRSDRTSLLVGLISLAGAVNTFNIHYPILTGPLYFTPNPFLLNFISAVSF
                     SVLFFMIFKSLFPDDFSTPALRLYQVVAAGVIASLFFVGFRTSERIVSAFYILTLAYL
                     LYQTVMLAKIMLYRRKDAVLFFIGFIAVNGTFVNNLLYALQIIDTGNIMQYAMVVFCV
                     STTAVISRRFARAIETVEEMSRELTEKNIALEKTDRLKDQFLAATSHELRTPLHGMIG
                     LSNSLLDGAAGGLSERARDIIALIAASGNRLANMVNDLLDMAKIQDEGFSLDLRPVDL
                     QALSAVVMRLALPLLGERPVEIVNRITPDLPCAWADEDRVRQVLSN"
     gene            complement(1953..>3384)
                     /locus_tag="PKJ16_18165"
     CDS             complement(1953..>3384)
                     /locus_tag="PKJ16_18165"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HNV48973.1"
                     /translation="VTAAPNVGYHFTSWSDGGVIATRTDTNITGDLTVTASFAIDQYT
                     VSFDSQGGSAVDPQLVNYGGLAIEPTAPSRTGYAFLGWHKESACTTPWAFAGDIVTGA
                     TTLYAKWGTAGELDTSLDPGTGANDNIHSIAIQSDQKIIIGGEFTDFDGTSIIRLARL
                     NANGSLDTTFNPGSGPYIMWQTFSYVYAIAIQSDGKIMIGGGFSHYNGTPVNHIARVN
                     IDGSLDTTFNQGSGCTGYVKALAIQSDGKILIGGSFSSYNGTSREKIVRVNADGSIDT
                     TFDPGSGAQDWPVNSIAIQSDGKIMIAGQFSYFNGTVRERMARLNANGSLDMSFTPGF
                     NANNTANSVAIQGDGKILVGGWFSNYNGSWSHSGRLNADGSIDTAFATNAGYGTSGNV
                     YSIVLQADGKILAGGIFTAYNLISRNRIARVNTDGTLDTTFVPGSGADAAINAIAIQS
                     NGYIVIGGAFTSYNGTARNRIARIIP"
BASE COUNT          674 a          928 c         1034 g          748 t
ORIGIN      
        1 ggttcgagag cacctgccgg acgcggtcct cgtcggccca ggcgcagggg aggtccgggg
       61 tgatgcggtt cacgatctcc acgggccgct cgcccaaaag cgggagtgcg aggcgcatca
      121 ccaccgcgct gagggcctgc aggtccaccg ggcgcaggtc cagcgagaac ccctcgtcct
      181 gtatcttggc catgtccagc aggtcgttca ccatgttcgc gaggcggttg ccgcttgccg
      241 cgatgagcgc gatgatgtcg cgcgcccgtt cggagaggcc gccggcggcg ccatccagga
      301 gggagttcga gaggccgatc atgccgtgca gcggcgtgcg cagttcgtgc gaggttgcgg
      361 caaggaactg gtctttgagc cggtccgtct tctcgagggc gatgttcttc tccgtgagct
      421 cacgcgacat ctcttcgacg gtttctatcg cccgggcgaa gcgccgggag atgaccgccg
      481 tggtggaaac gcagaagacg accatggcat actgcatgat gttgccggtg tcgatgatct
      541 gcagcgcata cagcagattg ttgacgaatg tgccgttcac ggcgataaat ccgatgaaga
      601 aaagcacggc gtccttgcgc cggtacagca tgatcttcgc cagcatgacg gtttgataca
      661 ggaggtaggc gagcgtgagg atgtagaagg cgctcacgat ccgttccgac gttcggaagc
      721 cgacgaagaa cagggacgcg atgacgcccg ccgcaacaac ctggtagagg cgcagggccg
      781 gcgtcgaaaa atcgtcgggg aacagcgatt tgaagatcat gaagaaaaga acggaaaacg
      841 aaacggcgct gatgaagttc agcaaaaacg ggttcggtgt aaaatagagc gggccggtga
      901 gaatgggata gtggatattg aaggtgttga ccgcccccgc aaggcttata agccccacga
      961 gcagagaggt cctgtccgat ctgcggaagc cgtagagcag catgttgtag atggcggtga
     1021 acagtgccag gccgatgatc atcatgtcga tggcgtggag gatgcacacg gtgcgcgcgt
     1081 ccgcgtcccg gtcattgagc gtgagcgccc ggtcgatacc gccgcagtca tggtcccggt
     1141 tgacgacctg cagcacgatc tcgttcacgc ccggcctgag cgggaacgat gctttgtatg
     1201 tgttgtgtat gtagatgaaa tcctcgcgtt tctccggggg cgatccgctc atggagcccc
     1261 tgccgccggc cggcacgccg ttgacgaaca gccggtaggc cgagtagatg cggtgtatcg
     1321 tcatggtctt ggcccggttg tccgggcccc gcaggatctt cagccgatag gtggcgcgcc
     1381 cctcgccggg taaggggttc ccattgaggt tttgtttttc ccacaggccg ggtacggtta
     1441 tgtagctgca gccgcgcgtg ttctccggcc tggtgaagtc cgccggcgca agctcccggt
     1501 cccagcagaa ctcccattcg ccgttcaggt tgatcgcgcc gtcgcgctcg aagtcccagt
     1561 ccctgaggtc gatgacgccc ttgaccgcga cgggccgctt ctccttcgag gacagcgggc
     1621 cgcagcccgc gcatgctgtc cagacgagga ggcacagggc caatgcggtg cacagccttc
     1681 gtgtcgataa tgggtgggtg ggcattgccc cattatatgc gggaaaaacg gcatgtcaag
     1741 tacgataggg acgggtctcg ttggggcagg gcagtgtcct gccccaggcc cagagacggg
     1801 gcgatgcccc gtctgtaccg aacgaaaata gaggttcaat agaggttgat aagaggttga
     1861 atagaggttg atcgggacca attggccggg ataacgcggt attgattgag agcgtggcgc
     1921 tgctccgcct ttacaagcgg atatctgtgg ttttatggaa ttattcgcgc gatgcgattc
     1981 cgggccgttc cgttatagga agtgaacgct cctcctatga cgatgtaccc attgctctgg
     2041 atggcgatgg cgtttattgc ggcatctgcg ccgcttccgg gaacaaaggt cgtatcgagg
     2101 gttccatccg tgtttacacg cgcaatcctg tttcttgaaa tcaagttgta cgcggtaaat
     2161 attcctccgg ccagtatttt cccatctgcc tggaggacga tggaatatac attaccgctg
     2221 gttccgtacc cggcgttggt tgcaaaagcg gtatcaatgc ttccgtcggc attcaaacgc
     2281 ccggaatgac tccatgagcc attataattg ctgaaccatc ccccaaccag gatctttcca
     2341 tcgccttgaa tggcaacaga atttgctgta ttatttgcat tgaatcctgg agtgaacgac
     2401 atgtcgaggc tgccgttggc attcaggcgc gccattctct cgcgtaccgt tccattgaaa
     2461 taggaaaatt gtcccgcaat cattatcttc ccgtcgctct ggatggcgat ggagttcacc
     2521 ggccaatctt gcgcgcctga tccgggatcg aaggtcgtat cgatgctccc gtccgcattc
     2581 acgcgtacga tcttttccct tgacgtgccg ttgtaggaag aaaacgatcc tccaattaaa
     2641 attttcccat cgctctggat ggcaagggct tttacatagc cggtacagcc tgacccttga
     2701 ttgaacgtcg tatcgaggct gccgtcgata ttcacacgtg cgatgtgatt tactggcgtt
     2761 ccgttgtaat gggaaaatcc tcctccgatc attattttcc catcgctctg gatggcgatg
     2821 gcatagacat acgaaaaggt ttgccacatg atatagggac cggatccggg attgaaggtt
     2881 gtatccaggc tcccgttcgc gttcaaacgc gccaacctga ttatggatgt tccatcaaag
     2941 tcggtaaact ctccgccaat gatgattttc tggtcgctct gtatggcgat tgagtgaata
     3001 ttgtcattgg cgccggtccc gggatccagc gaagtgtcaa gctctcccgc agttccccat
     3061 ttcgcataca gcgtcgtcgc ccctgtgacg atatcgccgg caaatgccca cggcgtggtg
     3121 caggcgctct ccttgtgcca gcccagaaat gcgtaccctg tccttgatgg cgcagtcggc
     3181 tctatggcca gtccgccgta attcaccagc tgtgggtcga ccgcgcttcc gccctgtgaa
     3241 tcgaatgata cggtatactg gtctatcgcg aagctcgcgg taacggttag gtcgccggta
     3301 atgttcgtgt ccgtccgcgt ggcaataacc cctccgtcgc tccacgaggt gaagtgatag
     3361 ccgacgttcg gcgcggcggt gacg
//
LOCUS       DAORHF010000352         3362 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31810_length_3362_cov_3.568793, whole genome shotgun sequence.
ACCESSION   DAORHF010000352 DAORHF010000000
VERSION     DAORHF010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31810_length_3362_cov_3.568793"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PKJ16_18170"
     CDS             complement(<1..1183)
                     /locus_tag="PKJ16_18170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48974.1"
                     /translation="MIPKQIEKLRTALASGEIPPRLPVIVASFVGLIIVAAILVFQSQ
                     KERIRSGRSEDLAAISRLKAAHIATWYAERMGDAMLLSHTAHWRDSSRAYLATRSGAE
                     MSHLREHMRDIVRHYGYADIIITDAAGTVLFGVDDPIPHISSYVRTLIARAVRENTVL
                     FSDIYRCPDDGDIFCDFIVPLSGAIAAPGPVSTCAVIRVDPRAFLFPLIQEWPTPSET
                     AETLLVRRDGDDVLYLNELRHRKVTALSLRNPINRPALPTAMAVRGVTGIVEGMDYRG
                     VEVLADLRGIDGTPWHLVSKVDISEVYRSVRVLFWMILVIGTLVIAGTLSIAGYWWKR
                     RSANYFRRQYEAEVRLRALRFHYEYLTRYANDIIVLGDENLRIIEANDRAVEVYGYSR
                     KE"
     gene            complement(1309..2619)
                     /locus_tag="PKJ16_18175"
     CDS             complement(1309..2619)
                     /locus_tag="PKJ16_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011792054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HNV48975.1"
                     /translation="MITAQNKLGLIFFPAFDWAISPTHPEREERLLYTQDQLREEGVF
                     DIAGVTEYKPLVASEEDVARVHFCLPDVASVITESHLISAGGAIKAGELVMDKTEQKA
                     FALVRPPGHHAMKVVHGNRGFCNINIEAVMVERLRERYGPLRVAIVDTDCHHGDGTQD
                     IYWHDPNTLYISIHQDGRTLYPGSGFTFEDGGPNARGRTINIPLPPETSDEGFLYAIE
                     RMVLPILTDFKPDLVVNSAGQDNHYSDPITNMNFSAQGYARLSELLNADIAVLEGGYS
                     IQGALPYVNLGIVLAMAGVDYTAVREPTYDAACIEQPRNIMPFIKKVADEVLAAYFNP
                     SGERGMPGNYDVRRKGVFYDTDMIREEQVESIMICDECAGTLKIETSSSANPLSLCVE
                     IPIGACDRCRDEGLRIVHEGKKKKGKDYAHIVLINRRDREYVRT"
     gene            complement(2630..>3362)
                     /locus_tag="PKJ16_18180"
     CDS             complement(2630..>3362)
                     /locus_tag="PKJ16_18180"
                     /inference="COORDINATES: protein motif:HMM:NF014071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HNV48976.1"
                     /translation="GTSVTAGPERRGPSMADGGSAPTLIDAFNVKKLAAYGDTAASEK
                     GVRALAKKSGRSPEDLAGAAIDYAMRAIKEASDAMVRAINEKPVYTVHEVLEGKTIVP
                     KRLYVMGGPARAFSDALAKRFGLPITVPEQYAVANAVGAALTHATMDIELFADTERGV
                     MLIPFLNVNERVSGSYTLEHAKKDAQARLADHLAGLGVKSAECASEIIEAASFNMVGG
                     WSTTGRNIRVKCQVKPGIVKGYRVG"
BASE COUNT          539 a         1055 c         1151 g          617 t
ORIGIN      
        1 gctccttgcg cgaatacccg tagacctcca cggcgcggtc gttcgcctcg atgatccgca
       61 ggttctcgtc gcccagcacg atgatgtcgt tggcgtagcg ggtgaggtat tcgtagtgga
      121 agcgcaatgc gcggagtcgc acctcggcct cgtactgcct gcggaagtag ttcgccgagc
      181 ggcgtttcca ccagtacccc gcgattgaga gggtgcccgc gatgacgagg gttccgatga
      241 cgagtatcat ccagaatagg acgcgtaccg atcggtacac ctccgatatg tcgaccttcg
      301 agacgaggtg ccatggcgtc ccgtcgatgc cgcgcaagtc cgccagcacc tcgaccccgc
      361 ggtagtccat gccctcgacg atgcccgtga cgccccgcac cgccatcgcg gtggggagcg
      421 caggcctgtt gattgggttt cgcagggaga gggccgtgac cttccgatgc cggagctcgt
      481 tcaggtacag aacgtcatcg ccatcgcgcc gcaccaggag cgtctcggcc gtctcgctgg
      541 gcgtgggcca ttcctggatg agcggaaaga ggaacgcgcg cgggtcgacg cggatcaccg
      601 cgcaggtcga tacgggcccc ggtgcggcga tggccccgga aagcgggacg atgaagtcgc
      661 agaagatgtc cccgtcgtcc gggcagcgat agatgtcgga gaagagaacg gtgttctcgc
      721 gcacggccct tgcgatgagc gtacggacgt atgagctaat atgcgggatc gggtcgtcga
      781 cgccgaagag gacggtcccg gcggcgtcgg tgatgatgat gtcggcatag ccatagtgcc
      841 gcacgatgtc gcgcatgtgc tcgcgcaggt gagacatctc ggcgccgctg cgggtcgcca
      901 ggtacgcgcg ggagctgtcg cgccagtgcg cggtatggct caacagcatc gcgtcgccca
      961 tgcgttctgc gtaccaggtg gcgatgtgcg ccgccttgag gcgggagatg gccgcaaggt
     1021 cctccgaccg acccgagcgg atccgttcct tctgggactg gaagacgagg atggccgcaa
     1081 cgatgatgag tccgacgaac gacgcgacga tgacgggcag acggggaggg atctccccgc
     1141 tcgccagtgc cgtgcgcagc ttttcgattt gtttcgggat catacacggt tctcgcgacg
     1201 cgctcggccg gaggcagcct ggggcggccg cgaaccgggc atcgtcacgc tgccggaagc
     1261 atggcggatc gggcccggaa aagcaataac aaaatgcccg ttacaccgct aggtccggac
     1321 gtactcgcgg tcgcggcggt tgatgaggac gatatgcgca tagtccttcc ccttcttctt
     1381 cttgccctcg tggacgatgc gcagtccctc gtcgcggcag cgatcgcacg ccccgatggg
     1441 tatctccacg cacagcgaaa gcggattcgc ggacgatgag gtttcgatct tcaaggtccc
     1501 cgcgcactcg tcgcagatca tgatcgattc cacctgctcc tcgcggatca tgtcggtgtc
     1561 gtagaagacg ccctttctgc gcacgtcgta gttgccgggc atcccccgct cgcccgacgg
     1621 attgaaatac gcggccagga cctcatcggc caccttcttt atgaaaggca tgatgttcct
     1681 cggctgctcg atgcacgcag catcgtaggt gggctcccgc acggccgtgt aatcaacccc
     1741 ggccatcgcg agcacgatcc ccaggttcac gtacggcagc gcgccctgga tggagtagcc
     1801 gccctcgagc acggcgatgt cggcgttcaa gagctcggag aggcgcgcat agccctgcgc
     1861 cgagaagttc atgttggtga tggggtcgga gtagtggttg tcctgcccgg cggagttcac
     1921 cactaggtcg ggcttgaagt ccgtgaggat gggcagcacc atgcgctcga tcgcgtacag
     1981 gaacccctcg tcggaggtct ccggggggag ggggatgttg atggtgcgcc cgcgcgcatt
     2041 gggcccgccg tcctcgaagg tgaaccccga gcccggatac agcgtgcggc cgtcctggtg
     2101 gatggagatg tagagcgtgt tcggatcgtg ccagtaaatg tcttgggtgc cgtcgccgtg
     2161 atggcagtcg gtgtccacga tcgcgacgcg cagcggtccg tagcgctcgc gcaggcgctc
     2221 caccatcacc gcctcgatgt tgatgttgca gaagccgcgg ttgccgtgca ccaccttcat
     2281 ggcgtggtgc ccgggcgggc gcaccagggc gaaggccttc tgctcggtct tgtccatcac
     2341 cagctcgccg gccttgatgg ccccgcctgc cgagatgagg tgcgactcgg tgatcacgct
     2401 cgcgacgtcc ggcaggcaga agtggacgcg cgcgacgtcc tcctcgctcg cgacgagcgg
     2461 cttgtactcc gtgacgcccg cgatgtcgaa gacgccctcc tcgcgaagct ggtcctgcgt
     2521 atacagcagc cgctcctcgc gctccgggtg cgtggggctg atggcccagt cgaacgccgg
     2581 gaagaagatg aggccgagtt tgttctgtgc ggttatcatt gctgtttcct caccccactc
     2641 gatatccctt cacgatgccc ggcttcacct ggcacttcac gcggatgttc cgtccggtgg
     2701 tggaccagcc gcccaccatg ttgaaggagg cggcctcgat gatctcggag gcgcactccg
     2761 cggatttgac gcccagcccg gcgagatggt ccgccagtcg cgcctgcgcg tccttcttcg
     2821 cgtgctccag ggtgtaggag ccggagacgc gctcgttcac gttgaggaag gggatgagca
     2881 tcacgccccg ctccgtgtcg gcgaagagct cgatgtccat ggtcgcgtgc gtgagcgcgg
     2941 cgccgacggc attggccacc gcgtactgct cgggcacggt gatcggcagg ccgaagcgtt
     3001 tcgcgagggc gtcggagaag gcgcgcgcgg gccctcccat cacatagagg cgtttcggga
     3061 cgatcgtctt tccctcgagc acctcgtgga cggtgtacac gggcttctcg ttgatcgcgc
     3121 gcaccatggc gtcgctcgcc tccttgatcg cgcgcatggc gtagtcgatg gcggcgccgg
     3181 ccagatcctc cggggagcgg ccgctcttct ttgcgagcgc gcggacgccc ttctcggagg
     3241 cggcggtgtc gccgtaggcg gcgagcttct tcacgttgaa ggcgtcgatg agcgtgggag
     3301 cggaaccgcc atcggccatg gaggggccgc gccgctcggg tcccgcggtg acggacgtgc
     3361 cg
//
LOCUS       DAORHF010000353         3358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31869_length_3358_cov_5.160763, whole genome shotgun sequence.
ACCESSION   DAORHF010000353 DAORHF010000000
VERSION     DAORHF010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31869_length_3358_cov_5.160763"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="PKJ16_18185"
     CDS             complement(<1..1236)
                     /locus_tag="PKJ16_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HNV48977.1"
                     /translation="MSEFVKISIGDLARQTAGKFPNNEALICPEFGVRETYAQFLDNC
                     VRTAKGFMALGIQRGERVSVWTTNLPEWVYLQFGLSMIGAVLVTVNTNYKSHELEYIL
                     KQSDSTTLVLMDSHRDTNYYDIAREVVRNLENTAPGQTKSDNLPLVKNVVYIGKRESV
                     PGMYRFSDVLALGESVSDADLAARERSIDVHDTINMQYTSGTTGFPKGVELTHYNIVN
                     NARMVGDVMGMTEKDKLLIQVPLFHCFGCVMSTLNSVYHGSTMVVLEAFDPAKAAKAI
                     QDERCTAINGVPTMFIAIMSHPDFAKYDVKSLRTGIMAGAPCPVEAMNDVRTKMHCSE
                     VVIAFGQTECSPVMTMTRRDDPVELRVSTVGRLLPDIEGKIVDPETGEDMPPKTQGEI
                     VTRSECVMKGYYKKPEATEK"
     gene            complement(1429..1773)
                     /locus_tag="PKJ16_18190"
     CDS             complement(1429..1773)
                     /locus_tag="PKJ16_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV48978.1"
                     /translation="MGRFVTMRDSGDVLEVEICSEYLDFSNASIICNQVVKILARHGY
                     PNSVIDISHVQEMSSAGVGFLMHVKDLLVRNGSWMKLRCENHALFDFMCFLHVNPLFE
                     ILPSEALAPSAL"
     gene            complement(1971..>3358)
                     /locus_tag="PKJ16_18195"
     CDS             complement(1971..>3358)
                     /locus_tag="PKJ16_18195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV48979.1"
                     /translation="VNDSIAELGYDPRELIGKHFDTIIQPEDRDAIDRMIYFGGDTVG
                     KPARQPKFFNERRTNERITRNLRVRLRPRSPAANREPILGEVFASGLYKPGPERGKKF
                     IGTVGIIRYVNESLRSAKTLSKTEQYYRLLIENSFEIIMITAHDGTVLYASSSVERVL
                     GRASFGLIGESIRDIVHLDDVRAVEAAIGGRRAGQRPRRRMEFRLVDGAGGVKHFEAA
                     ITMIADECHGAAMCYVFHAIDITARKEIESAMEKRERIYKILLRTSPDAIVLFDADGD
                     IIMANDRASELLGQRREELIGRHFMSFLFEKEKARASNLRRRMVLARVMQSYFLNNVF
                     TLRRDDGGELPVEVSFSAINDPGGIVEGYLAVIRDITERRRAEEDKRRLEEELLRIVT
                     SRLSGREIELLKYIHDGLSWPAHKREIGKAMDVIPGTLDKFVSRIKQKMGAADMDEIV
                     NIAALRFKWFR"
BASE COUNT          647 a         1036 c          942 g          733 t
ORIGIN      
        1 cttttcggtg gcctcgggct tcttgtagta gcccttcatg acgcactcgc tgcgcgtcac
       61 gatctcgccc tgcgtcttcg gcggcatgtc ctcgccggtc tcgggatcga cgatcttccc
      121 ctcgatgtcg ggcaacaggc ggcccacggt ggaaacccgc agctccaccg gatcgtcgcg
      181 ccgcgtcatc gtcatcacgg gcgagcactc ggtctggccg aaggcgatca ccacctcgga
      241 gcagtgcatc tttgtgcgca cgtcgttcat cgcttccacc gggcagggcg cgccggccat
      301 gatgcccgta cgcagcgact tcacatcgta cttcgcgaaa tcgggatggc tcatgatggc
      361 gatgaacatc gtgggcacgc cgttaatggc cgtgcatcgc tcgtcctgga tcgctttcgc
      421 cgcctttgcg ggatcgaacg cctccagcac caccatggtg gacccgtgat acacggagtt
      481 gagcgtgctc atcacgcagc cgaagcagtg aaagagcggg acttggataa gcaacttgtc
      541 cttctccgtc atgcccatca cgtcgcccac catccgggcg ttgttcacga tattataatg
      601 ggtaagctcg acccccttgg gaaatccggt ggtgcccgag gtatattgca tgttgatggt
      661 gtcgtgcacg tcgatcgagc gctcgcgcgc cgcaaggtcc gcgtctgaga ccgactcgcc
      721 aagcgcgagc acgtcggaga atcggtacat gccggggacc gattcgcgct ttccgatata
      781 gacgacattt ttcacgagcg gcaggttgtc cgatttcgtc tgtcccggcg cggtgttctc
      841 aaggttgcgg accacctcgc gcgcgatatc gtagtaattg gtgtcgcgat gcgagtccat
      901 gagcaccagg gtcgtcgagt ccgactgctt caggatatac tcgagctcgt gcgacttgta
      961 attggtgttc accgtcacca ggaccgcgcc aatcatgctg agaccgaact gcaggtacac
     1021 ccactccgga aggttcgttg tccacactga gacgcgctcg ccgcgctgga tgccaagcgc
     1081 catgaagccc ttcgcggtcc tgacgcagtt gtccaggaac tgcgcgtatg tctcacgcac
     1141 cccgaattcg gggcagatga gcgcctcatt gttgggaaat tttccggcgg tctgacgcgc
     1201 caggtcgcca atgctgattt ttacaaactc cgacatatgc ctccccctgc actgatttga
     1261 aatagaactg tacgatgcat acttcgtaat ccagttaacg ttaactggca agcttgggga
     1321 gcaaaccgtt tttgtcaagg gataatccac gatttttgtc attcacgcgg aggcttcggg
     1381 agggggcgca catatccccg agcaatcgca gcggcagcgg ggatggcgct acagcgcgga
     1441 cggcgccagc gcttcagagg gaaggatctc gaacagcggg ttaacgtgca gaaagcacat
     1501 gaaatcgaac agggcgtgat tttcgcagcg aagcttcatc cagcttccat tccgcacgag
     1561 aagatctttc acatgcatca ggaaccccac cccggcggag ctcatctcct gaacatgcga
     1621 tatatcgatt acggagttag gatagccgtg tcgtgcgaga atctttacaa cctggttaca
     1681 gatgatcgaa gcgttcgaaa agtcgagata ctccgagcat atctccacct cgaggacgtc
     1741 ccccgagtcg cgcatcgtga cgaatctacc cataaatact cccgaatcaa gttcaggcac
     1801 actcatgtcg ttccgcttat gtatctctgt ctattgtttt gatctcgtgc gacataaatc
     1861 attttcggcg acattcgcca ataattcgac gcttgtcaac aactagacat atttttttta
     1921 ctgacaattt ttgtcattta catatttcta attttgttgt tctttataat ctatcgaaac
     1981 cacttgaatc gaagggcggc gatgttcacg atttcatcca tgtcggcggc gcccatcttc
     2041 tgtttgatgc gcgagacgaa tttatcgagg gtgccgggga tcacgtccat cgccttgccg
     2101 atctcccgct tgtgcgccgg ccaggacagg ccgtcgtgaa tatatttcaa cagctcgatc
     2161 tcgcggccgg agagccggct ggtcacgatc cgcagaagct cctcctcgag ccgccgcttg
     2221 tcctcttcgg cgcgccgccg ttcggtgatg tcgcgaatga ccgcgagata cccctccacg
     2281 atcccgcccg gatcattgat ggccgaaaag ctcacctcga ccggcaactc gccgccgtcg
     2341 tcccgccgga gggtgaagac attgttcagg aagtagctct gcatgacccg cgcgagcacc
     2401 atccggcgcc gcaggttcga cgcgcgcgcc ttttccttct cgaacaggaa cgacatgaag
     2461 tggcgcccga tgagctcctc gcgccgctgc cccagaagct cgctcgcgcg gtcgttggcc
     2521 atgatgatgt ccccatcggc gtcgaacagg acgatcgcat cgggagaggt ccgcaggagg
     2581 atcttgtaga tgcgctcgcg cttctccatc gcgctctcga tctccttgcg cgccgtgatg
     2641 tcgatcgcgt gaaagacata gcacatggcg gcgccgtggc attcgtccgc gatcatggtg
     2701 atcgcggcct cgaagtgctt cacgcccccc gcgccgtcca cgagacgaaa ctccatccga
     2761 cgccgcggcc gctgccccgc ccgccggccg ccgatcgccg cctcgacggc ccgcacatcg
     2821 tcgagatgca cgatatcgcg gatgctctcg ccgatgagcc cgaagctggc gcgccccagc
     2881 acccgctcga ccgacgaact ggcgtagagc acggtgccgt cgtgcgcggt gatcatgatg
     2941 atctcgaagg aattttcgat gaggagccgg tagtactgct ccgtcttgct gagggttttt
     3001 gcgcttcgaa gcgactcatt gacatagcgg atgatgccca cggtgccgat aaatttcttc
     3061 ccgcgctccg gccccggctt gtacaggccg ctcgcgaaga cctcgcccag gatcggctcc
     3121 cggttcgcag ccggcgaccg gggacgcagc cgcaccctga gattccgcgt gatccgctca
     3181 ttggtccgcc gctcgttgaa aaacttcggc tgccgcgcgg gtttcccgac ggtatcgccg
     3241 ccgaaataga tcatgcggtc gatcgcatcc cggtcctccg gctgaatgat ggtgtcgaaa
     3301 tgctttccga taagttcgcg cggatcatac ccgagttcgg cgatggagtc gttcacat
//
LOCUS       DAORHF010000354         3353 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_31940_length_3353_cov_4.176774, whole genome shotgun sequence.
ACCESSION   DAORHF010000354 DAORHF010000000
VERSION     DAORHF010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31940_length_3353_cov_4.176774"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PKJ16_18200"
     CDS             <1..233
                     /locus_tag="PKJ16_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48980.1"
                     /translation="RERTQQRHHAAITRVAGVSQDVTQREGVLETSVHVAGLGPEALR
                     DLQVIVRTMMIGLDEVRRLHPESITIDFDCEV"
     gene            236..496
                     /gene="rpmA"
                     /locus_tag="PKJ16_18205"
     CDS             236..496
                     /gene="rpmA"
                     /locus_tag="PKJ16_18205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015168307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HNV48981.1"
                     /translation="MAHKKGGGSTRNGRDSNSQRLGVKRFGGERVKGGTVLVRQRGTR
                     IHPGTNVGLGKDDTLFAKIDGIVTFERLDKTRKKVSVYPVAP"
     gene            616..1623
                     /gene="obgE"
                     /locus_tag="PKJ16_18210"
     CDS             616..1623
                     /gene="obgE"
                     /locus_tag="PKJ16_18210"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HNV48982.1"
                     /translation="MPKFTDTIVIRVKAGDGGPGSVSFFREKYVPNGGPDGGDGGKGG
                     DVYLEASSNHHNLSHLFKDRAYEAERGQFGMGLNRHGRNGKDLVVKVPVGTEVMDEES
                     GELVCDLTETGQRFLVAAGGIGGRGNAFFKSSTHQSPRFAQPGMPGEDKRLLLNLKLI
                     ADVGLVGLPNAGKSTLLSQLTNAKPKIADYPFTTLIPNLGVVAREEGRAYKIADIPGI
                     IEGAHRGMGLGLSFLMHIERVRAILYLIEATEADPAYNLELLRHELTAYSPRMIDKPY
                     YVVITKTDLVDADALRECIDKIGDDRVISISSVTRHNLDELYRVIDSLLETGGAPQGA
                     A"
     gene            1601..2710
                     /gene="proB"
                     /locus_tag="PKJ16_18215"
     CDS             1601..2710
                     /gene="proB"
                     /locus_tag="PKJ16_18215"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HNV48983.1"
                     /translation="MPRRELLKDVRRIVVKVGTSSLTEGEVLSPEKIARLANDIVGVV
                     RRGYQVVLVSSGAISAGAGLMSRRRDFVTIPEKQALAAVGQILLMDQYRACFNAAGLE
                     IGQILLTEDDVKNRRRYLNARHTFNALLEFGVVPIVNENDSVVIKEIKIGENDTLSAY
                     VANIIEAELLVLLSDVDGFYRDLADPEPIEEVFDITDEIRDLAGGAGSAHGSGGMSTK
                     IRAAEIVIGSGEMMIIANGAVEGVLGRIMNGEKIGTIFVGRDRALSSRKRWIAYSVKA
                     AGTVIIDDGAVRALRERKKSLLARGITGVQGDFDLGDAVDIVDARGERIGKGIVNYNA
                     KELQLIKGKKTDEIRDMLESNYFDEVVNRDDLIIY"
     gene            complement(2704..>3353)
                     /locus_tag="PKJ16_18220"
     CDS             complement(2704..>3353)
                     /locus_tag="PKJ16_18220"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV48984.1"
                     /translation="KIGGDFFSFTHLQEGGMGVFIGDVSGHGVSAALFLSLLKAETDH
                     VCRAHGLKPHDFIERLNANLIPSMPHHFVTAIYGRFYVNPDTGRIELSFANGGHPNPI
                     IHHRDSGIIETLDSHGPVIGKFPNAVFQNMKIDLRPGDRVFFYTDGIPESRNARNEYL
                     GFDRLPSIIEYTANANLDDSLEAIIQTVGRFRGGLAQEDDMVLIGCEVRSGGADQ"
BASE COUNT          677 a          997 c         1089 g          590 t
ORIGIN      
        1 accgtgaacg aacgcagcag cggcaccacg cggcgatcac cagggtcgcc ggcgtcagcc
       61 aggacgtgac gcagcgcgag ggggttctgg agacgagcgt gcatgtcgcg ggccttggcc
      121 cggaagcgct cagggacctt caggtcattg tacgaacgat gatgatcgga ctggatgagg
      181 tcaggcggct tcatccggag agcataacga tagatttcga ctgcgaggtg taatcatggc
      241 acataaaaaa ggcggcggtt caacacggaa cgggcgcgat tcgaattcgc aacggctggg
      301 cgtgaagcgc ttcggcggcg agcgcgtcaa gggcggcacg gtgctggtgc gccagcgcgg
      361 aacccgcatt catccgggca ccaacgtcgg tcttggaaag gacgatacgc tttttgcgaa
      421 aatcgacggg atcgtgacct tcgagcgctt ggataaaacg cgaaagaagg tgtcggtgta
      481 tcctgtggcg ccgtaagacc cccacccgac cgtccacacc gaaaaatcgc gcctggacaa
      541 atccggacgc gattttttgt ttcccgcgcg ggatgcgcga tggacgtccg gagcggtcga
      601 caatacacga atatcatgcc aaaattcacc gatacaatcg tcatacgcgt aaaggccggg
      661 gacggcgggc cgggctcggt ttcgttcttc agggagaagt atgttcccaa cggagggccc
      721 gacggcggcg atggcgggaa gggcggcgac gtgtatctcg aggcgtcgag caatcatcac
      781 aatctctcgc atctcttcaa ggaccgcgcc tacgaggccg agcgcggcca gttcggcatg
      841 gggctcaatc ggcatgggcg caacggcaag gaccttgtgg taaaagtccc tgtggggacc
      901 gaggtgatgg acgaggaaag cggcgagctg gtatgcgatc tcaccgaaac ggggcagcgc
      961 tttctggtcg ccgcgggagg gatcggcggg cggggaaacg ccttctttaa atcgtcgacc
     1021 catcaatccc cccgcttcgc gcaaccgggc atgccgggcg aggacaagcg cctgctgcta
     1081 aacctgaagc tcatcgctga cgtgggcctg gttggcctgc ccaatgccgg aaagtcgacc
     1141 ctgctgtccc agcttaccaa cgcgaagccg aagatcgccg attacccgtt cacgacgctc
     1201 atccccaatc tcggcgtggt cgcccgtgag gaaggccgcg cctacaagat cgcggacatc
     1261 cccggcatca tcgagggcgc ccaccgtggg atggggctgg gcctgtcgtt tctcatgcat
     1321 atcgagcgcg tccgcgccat tctgtatctc atcgaggcga ccgaggccga tccggcctac
     1381 aacctcgagc tattgcggca cgaactcacc gcgtacagcc cgcggatgat cgacaagccg
     1441 tactacgtcg tcatcaccaa gacggacctg gtcgatgcgg acgcgctccg ggaatgtatt
     1501 gacaaaatcg gggacgatcg ggtgatatcg atatcgtcag tgacccggca caatctcgac
     1561 gagctctacc gggtgataga ttcattgctg gagaccggtg gtgccccgca gggagctgct
     1621 taaagacgta cgccgcatag tcgtcaaggt ggggacctcg tccctgaccg agggggaggt
     1681 gctgtcgccc gagaaaatcg cgcggctggc caacgacatc gtcggggtcg tccgccgggg
     1741 gtaccaggtg gtgctcgtct cctccggcgc cattagcgcc ggcgcggggc tgatgagtcg
     1801 gcggcgcgat ttcgtgacca ttcccgagaa acaggcgctc gccgccgtcg gccagatcct
     1861 cctcatggac cagtaccgcg cctgcttcaa cgccgccggc ctcgagatcg gccagatcct
     1921 gctgaccgag gacgacgtga aaaaccgccg tcgctacctc aacgcgcgcc acaccttcaa
     1981 cgcccttctc gagttcggcg tggtccccat cgtcaacgag aacgattccg tggtcatcaa
     2041 ggagatcaag atcggcgaga acgacaccct ctcggcgtat gtggccaata tcatcgaggc
     2101 cgagctcttg gtgctgctgt ccgacgtgga cggtttttac cgcgatcttg cggacccgga
     2161 gccgatcgaa gaggtgttcg atatcaccga cgagatccgc gatctcgccg gcggggcggg
     2221 gagcgcgcac gggagcggcg gcatgtccac caagatccgc gcggcggaga tcgtcatcgg
     2281 ctccggcgag atgatgatca tcgccaacgg cgccgtcgag ggcgtgctcg gcaggatcat
     2341 gaacggcgag aagatcggca ccatcttcgt gggacgggac cgcgcgcttt ccagccgcaa
     2401 gcgctggatc gcctacagcg tgaaggctgc ggggaccgtg atcatcgacg acggcgccgt
     2461 gcgcgcgctt cgcgagcgga agaagtccct tctcgcgcgc ggcatcaccg gcgtgcaggg
     2521 cgatttcgac ctcggcgacg cggtggacat cgtggacgcg cgcggcgagc ggatcggcaa
     2581 ggggatcgtc aactacaatg cgaaggagct ccagctcatc aaggggaaaa aaacggacga
     2641 gatccgcgac atgctggaga gcaattattt cgacgaggtc gtcaatcgcg acgatctcat
     2701 catctactga tccgcaccgc cagagcgcac ctcgcacccg atgaggacca tgtcgtcctc
     2761 ctgcgcaagc cctccccgga accggccgac cgtttggatg atggcctcca gcgaatcgtc
     2821 gagattggcg ttggccgtgt attcgatgat ggacggaagt cggtcgaatc cgaggtattc
     2881 gttgcgcgca ttgcggcttt cgggtatgcc gtcggtatag aagaacaccc ggtcccccgg
     2941 ccgcaggtca attttcatgt tctggaagac ggcgttcggg aactttccga tcaccggtcc
     3001 gtggctatcg agggtctcaa tgatccccga gtcccggtgg tggatgatgg gattcgggtg
     3061 gccgccgttg gcgaagctga gttcgatgcg tcccgtgtcc ggattgacgt aaaagcgtcc
     3121 gtagatcgcg gtcacgaaat ggtgaggcat gctgggaatc aggttcgcat tgagccgttc
     3181 gatgaaatca tgcggcttga gcccgtgcgc gcggcagaca tggtcggtct cggccttgag
     3241 gagcgaaaga aacagcgccg ccgaaacccc gtgtccggaa acgtccccga tgaacacccc
     3301 catgcccccc tcctggaggt gcgtgaacga gaagaaatct ccgccgatct tgt
//
LOCUS       DAORHF010000355         3333 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_32230_length_3333_cov_4.067724, whole genome shotgun sequence.
ACCESSION   DAORHF010000355 DAORHF010000000
VERSION     DAORHF010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32230_length_3333_cov_4.067724"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>391)
                     /locus_tag="PKJ16_18225"
     CDS             complement(<1..>391)
                     /locus_tag="PKJ16_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012984284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNV48985.1"
                     /translation="GKNILGSGFDFDIKISRGGGAFVCGESSALMRSLEGKVGEPRAK
                     YIRSVEKGLYDKPSVLNNVETWNNVPVIMLNGAAWYAAMGTEGSKGTKVFSLVGKVNN
                     TGLVEVPMGITIGTSTRPVLFTLPTRE"
     assembly_gap    392..401
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<402..1116)
                     /locus_tag="PKJ16_18230"
     CDS             complement(<402..1116)
                     /locus_tag="PKJ16_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HNV48986.1"
                     /translation="MAAALEEEIKKQGLAVNVSIGLKTTGCHGYCENGPLVVLRPQDV
                     LYLKVKPKDVPEIVEKSIKEGAIIDRLVYKDVNTKAAIAKYHEIPFYAYQKRIALRNI
                     GTVDPCVIDDYLINDGYKALAKTLTMDPEAVIKEIEESGLRGRGGGGFPAGRKWRSCR
                     AVESDVRYVMCNGDEGDPGAFMDRSIMEGDPHSVIEGMIIGGYAVGARQGYVYVRDEY
                     PLAVKNLEIAIDAAREKGLL"
     gene            complement(1257..1715)
                     /locus_tag="PKJ16_18235"
     CDS             complement(1257..1715)
                     /locus_tag="PKJ16_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HNV48987.1"
                     /translation="MDVTIAKVDEIIGRYGKTEESILAVLQDFQREFRYVPEEGMKRL
                     AGVLDVPESRIYAMGTFYKALSLEPRGKHTVKVCMGTACHLKGANQILEAIERGFSVK
                     RNHTTKDKNFTVESVNCVGACAMAPVIVVDEDYHGHARSSKVVDILKKYN"
     gene            complement(1718..2644)
                     /locus_tag="PKJ16_18240"
     CDS             complement(1718..2644)
                     /locus_tag="PKJ16_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV48988.1"
                     /translation="MDKYSVYSARLVDQTEYMAPGHRACQGCAEVLAVRLILKALGRD
                     TIMAMSTGCMEIISSPLPLTSWRIPWIHNAFENSSAVVSGCESGMKAMMRKGRIPQKK
                     INFVAMGGDGATADIGMGPLSGALERGHDFLYVCLDNEAYMNTGIQRSSSTPYGASTT
                     TSPAGKKSIGQHTQKKNMAQIAVAHNIPYVATACTSYPFDLMEKAKRAAEMNGAAYLH
                     VLSVCPTGWRIPPEEAIKYGRLAVESGMFPLYEVVNGKYSLSYKPPVMIPVKEYLAAQ
                     GRYRHLTDTDLHKIEEMAMQEYQRIVRLSEGD"
     gene            complement(2655..>3333)
                     /locus_tag="PKJ16_18245"
     CDS             complement(2655..>3333)
                     /locus_tag="PKJ16_18245"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HNV48989.1"
                     /translation="LDGFIMTHMVEPVEYVDGELIKKYLPEFKMKNALHPDNPVSMGC
                     FAMPEIYTEAQMSREVGIKDAYPTIVKAWDEWGALTGRHYKPIETYRTDDADYCIITM
                     GSFGETAMEVADMLREEGEKVGVVKIRLWRPFPMEEFRKSVLGKKYLIVLDRAISFGS
                     QGGPVAMELRSALYGQKDAPVVVNYVAGLAGRDVSRKDFRNIILEGKKKAVSGHTDGF
                     TLYGLRG"
BASE COUNT          638 a         1039 c          852 g          794 t
ORIGIN      
        1 ttttccctgg tgggcaaggt gaacaacacc ggtctcgtgg aagtgccgat ggtgatcccc
       61 atcggcactt ccacgagacc ggtgttgttc accttgccca ccagggaaaa gaccttggtg
      121 cctttcgatc cctcggtgcc cattgcggcg taccatgcgg cgccgtttaa cataatgacc
      181 ggaacgttgt tccacgtctc tacattgtta aggaccgacg gcttgtcgta caggcccttt
      241 tcgacagagc ggatgtattt tgcgcgcggt tcgccgactt ttccctcgag ggagcgcatg
      301 agcgccgatg actccccgca cacgaaggcg ccgccgccac ggctgatttt aatgtcgaaa
      361 tcgaaccccg aaccgagaat gtttttcccc annnnnnnnn nccaacaagc ccttctcccg
      421 ggctgcgtcg attgcgattt cgaggttttt tacggcgagc ggatattcat cgcgcacgta
      481 gacgtagccc tggcgggccc cgaccgcgta tccgccaatg atcattcctt caatcaccga
      541 atgcgggtcc ccctccatga tggagcggtc cataaacgcg ccgggatcgc cttcgtcgcc
      601 attgcacatc acatagcgca cgtcggattc aactgctcgg catgagcgcc actttcttcc
      661 cgcgggaaat cccccgccgc cccggccccg gaggcccgat tcctcgattt ctttaataac
      721 cgcttccggg tccatggtga gcgtcttggc cagcgccttg tatccgtcat tgatgagata
      781 atcgtcgatc acgcagggat cgacggttcc gatattgcga agcgcgatgc gcttctgata
      841 ggcgtaaaaa gggatctcgt gatatttcgc gatcgccgct ttcgtattca cgtccttgta
      901 cacgagtcgg tcgataatcg ccccttcctt gatcgacttc tcgacgattt ccggcacgtc
      961 cttgggtttc accttcaaat acaatacgtc ctgcgggcga agcaccacca gcggcccgtt
     1021 ctcgcagtac ccgtgacaac cggtcgtctt gagaccgatg gaaacattga cggcaagccc
     1081 ctgtttcttt atttcttcct ccagggctgc ggccacgtca cgcgctccgt tcgcctgaca
     1141 gccggtccca aagcacacca ataccgatgg aagatcgtgc ttcacgccgg ccctgagcgt
     1201 ctctcgtact ttttctagct gatctgctga ttgaattgtc gccatctgcc gtacccctaa
     1261 ttgtatttct tgagtatgtc gacgaccttc gacgatcgag catggccgtg ataatcctcg
     1321 tccacgacga tcaccggcgc catcgcgcac gcaccaacgc agttgacgct ctcaacggtg
     1381 aaattcttat cttttgtggt atggttccgt ttgacgctaa acccgcgctc aatcgcctct
     1441 aaaatctggt tcgcgccctt gagatggcat gcggtcccca tgcacacctt cacggtatgc
     1501 ttgccgcgag gctccagcga cagcgccttg tagaaggtgc ccatggcata aatgcggctt
     1561 tcggggacat cgagcactcc ggcgaggcgc ttcatccctt cttcgggaac atagcggaac
     1621 tcgcgctgaa aatcctgcag aaccgcaagg atggattctt ccgtttttcc gtatcgtccg
     1681 atgatttcat cgacttttgc gattgtcaca tccatgatca gtctccttcg ctcaatctga
     1741 cgattctctg atactcctgc atcgccattt cttcgatttt atgaagatcc gtatcggtga
     1801 ggtggcggta ccgtccctgc gcggcgaggt attcctttac ggggatcatc accggaggct
     1861 tgtacgaaag gctgtatttg ccattcacca cttcatagag ggggaacatc cccgattcaa
     1921 ccgccaggcg cccgtatttg atcgcttctt ccggaggaat ccgccagccg gtcgggcaga
     1981 ccgacagcac gtgcagatag gccgcgccgt tcatttcggc cgcccgcttc gctttctcca
     2041 tcaggtcgaa cggatagctc gtgcacgccg tcgcgacata cggtatattg tgggcaacgg
     2101 cgatctgcgc catgttcttc ttctgcgtgt gctggccgat gcttttcttt cccgcagggc
     2161 tcgtggtggt gcttgccccg tacggcgtgg aactcgagcg ctggatcccg gtgttcatgt
     2221 atgcttcatt atccaaacag acatacagaa aatcatgacc gcgctcgagc gccccggata
     2281 acggccccat cccgatatcg gcagtcgcgc catcaccgcc catcgccacg aaattaatct
     2341 tcttctgggg gattcggccc ttgcgcatca tcgccttcat ccctgattca cagccggata
     2401 cgaccgcgga tgagttttcg aacgcgttgt gtatccacgg aattcgccat gatgtcaacg
     2461 gcagcggcga tgaaatgatt tccatgcaac cggttgacat cgccatgatg gtatcacggc
     2521 cgagcgcctt gagaatcaac cgcaccgcca acacctcggc gcacccctgg cacgcccggt
     2581 gcccgggcgc catatattcg gtttgatcga caagccgggc ggaataaaca gaatatttat
     2641 ccatatggtt ctccttatcc tctcaagccg tacagggtga agccgtcggt gtgacccgat
     2701 accgccttct ttttgccctc taaaatgatg tttctaaaat ccttccgcga cacgtcccta
     2761 cccgcaaggc cggcgacgta gttcaccacg acgggggcgt ccttttgccc gtagagcgcg
     2821 gaacgcagct ccatcgccac cggcccgccc tgcgagccga agctgattgc gcggtcgagg
     2881 acaatgaggt acttcttccc gagaaccgat ttccggaact cctccatcgg gaacggccgc
     2941 cagaggcgta tcttcaccac gccgaccttc tccccttcct cgcgaagcat atcggccact
     3001 tccatggcgg tttcgccgaa cgatcccatc gtgatgatgc agtagtcggc atcatcggtg
     3061 cggtatgtct caatcggctt gtagtgccgg cccgtgagcg cgccccattc gtcccacgcc
     3121 ttcacgatgg tcgggtacgc gtctttgatg ccgacctcgc gcgacatctg cgcctcggtg
     3181 tagatttccg gcatcgcgaa acagcccatc gacaccgggt tgtcgggatg cagggcattt
     3241 ttcattttga attcaggcag atacttcttg atgagttcgc catcaacata ttccaccggt
     3301 tcgaccatgt gcgtcatgat gaacccgtcg agg
//
LOCUS       DAORHF010000356         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_32589_length_3309_cov_6.036263, whole genome shotgun sequence.
ACCESSION   DAORHF010000356 DAORHF010000000
VERSION     DAORHF010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32589_length_3309_cov_6.036263"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PKJ16_18250"
     CDS             complement(<1..1139)
                     /locus_tag="PKJ16_18250"
                     /inference="COORDINATES: protein motif:HMM:NF014474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HNV48990.1"
                     /translation="MTPSANSITVGLIGNPNCGKTTIFNALTGARQKVANWSGVTIEK
                     REGSFRTGEYSVRVVDLPGIYSLSAYSLEEIVARDYIIHEQPDVIVNIVDAGNLERNL
                     YLTTQLINIGVRVILVLNMSDEAEAKGIVIDTDTLGTLLGMPVVRTVGTRNDGIGRLI
                     AAIIAVAEKTAPVVRHLHIDYGADVEEEIQAVQRAIRTVGDIGERYSTRWLAIRLLEN
                     DCDVLAKLEGFPNYAAIVGQAERSRRILEKNYGDDPAMVLTERRQGFVTGLLKEAVRR
                     VSVEKTDVSERIDRVLMNRYLGYPILLAFLWLLFQATYVLGEYPKQGIQWLVDALSHW
                     LALSLPDGAVKSLVVKGVVGGVGGVIVFLPNILILFLGVSFLEDSG"
     gene            complement(1136..1834)
                     /locus_tag="PKJ16_18255"
     CDS             complement(1136..1834)
                     /locus_tag="PKJ16_18255"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HNV48991.1"
                     /translation="MKIDHARDMFESTDLSSNMEDYLETIAVLSRAHRVVRVKDIAKS
                     LKVKMPSVTAALGKLSEQGLVNYEKYGYVDLTERGKAIADKVYSRHECLMDFFSEVLR
                     MDAETADGEACRMEHHLAPQSCRQLYKLVEYFKRADAQSPWRKELADLLDERPLSELS
                     EGDEAMIVRIEAEGVLKKRLIELGFRRGASIALVRYAPLQDPLQVTIQKYSISLRVEE
                     ARAVIVKLKAKTVE"
     gene            2007..2525
                     /gene="cobU"
                     /locus_tag="PKJ16_18260"
     CDS             2007..2525
                     /gene="cobU"
                     /locus_tag="PKJ16_18260"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013557239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HNV48992.1"
                     /translation="MGHTIFITGGCRSGKSTHALARAQALGAETVFIATCRPGDAEME
                     RRIARHRAERPAAWRTVEEYGPLADVLRSETQRDSAIILDCLTLYVSNLLVSDMPTGG
                     IIENIREIAEIIAQGAANAIVVSNEVGWGIVPDNALARDFRDIAGTANQIVAARANEV
                     ICMIAGIPMRIK"
     gene            2522..>3309
                     /locus_tag="PKJ16_18265"
     CDS             2522..>3309
                     /locus_tag="PKJ16_18265"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HNV48993.1"
                     /translation="MKPGNTHELADIIAAVRPPRDTLMAQALARLDNLTKPRGSLGRL
                     EEIAVRIACIAGRIDPQLANKVVFTMAADHGVADENVSLYPKAVTEQMVYNFLRGGAA
                     VNVLARHAGARVVVVDMGVAADIADPGGTLAIRKIAYGTASMTKGPAMTRDEAIRAIR
                     AGFEVVAEEHARRPIDIVALGDMGIANTTASSAIVSCITALPPEETTGRGTGIDDERL
                     AWKIGEIETALDINRPDPADPIDVLAKVGGFEIGALAGVALAAAA"
BASE COUNT          608 a         1086 c         1022 g          593 t
ORIGIN      
        1 ccgctgtcct ccaggaagct cacgcccaaa aacaggatga gaatgttcgg caggaacacg
       61 atcacgcccc ccaccccgcc cacaacgccc ttgaccacca ggctcttcac ggcgccgtcg
      121 ggcagggaca gcgcgagcca gtgcgacagc gcgtccacca gccactggat gccctgcttg
      181 ggatattcgc ccagcacata agtcgcctgg aacagcagcc acaggaaggc gagcagaatg
      241 ggatagccca gatagcggtt catgaggacc cggtcgatgc gctccgagac gtccgtcttt
      301 tcgacgctca cacggcgtac cgcctctttc aggaggcccg tcacgaatcc ctgtcgccgc
      361 tccgtgagga ccatcgccgg atcgtcgccg tagttctttt cgaggatgcg ccgggagcgc
      421 tctgcctgcc cgacgatggc cgcgtagttg ggaaagccct ccagcttggc cagcacgtcg
      481 caatcgttct cgagcaggcg gatggcgagc cagcgcgtgg agtagcgctc gccgatatcg
      541 cccaccgtcc ggatcgcgcg ctggaccgcc tggatctcct cctcgacatc ggccccgtag
      601 tcgatgtgca gatggcgcac cacgggcgcc gtcttttccg ccaccgcgat gatggccgcg
      661 atgagccggc cgatcccgtc attgcgcgtc cccacggtgc gcacaaccgg catccccagc
      721 agcgtcccca gcgtatcggt gtcgatcacg atgcccttgg cctcggcctc gtcggacata
      781 ttcaggacga ggatcacccg cacgccgatg ttgatgagct gtgtggtgag atacaggttt
      841 cgctcgaggt tgccggcgtc cacgatgttc acgatcacgt ccggctgttc atggatgata
      901 tagtcgcgcg cgacgatctc ctcgagcgaa taggccgaca ggctgtagat accgggcagg
      961 tccaccacgc gaacggaata ctccccggtc cgaaaagagc cctcgcgctt ttcaatggtc
     1021 accccgctcc agttcgcgac cttctgccgc gcgccggtga gggcgttgaa aatggtggtc
     1081 tttccgcagt tgggatttcc gatgagtccg acggtgatgc tgttcgcgct cggggtcatt
     1141 cgacggtttt cgctttcagt ttaacgatga ccgctcgggc ctcctccacc cgaagcgaga
     1201 tgctgtattt ctgtatcgtg acctgcagcg ggtcctgcag cggggcgtag cgcaccagcg
     1261 caatcgacgc gccgcggcga aaacccagtt cgatgaggcg ctttttaaga acgccctcgg
     1321 cctcgatgcg cacgatcatc gcctcgtcgc cctcgctcag ttcgctcaac gggcgctcgt
     1381 ccaggagatc ggcaagctcc ttcctccacg ggctctgcgc atcggcgcgc ttgaaatatt
     1441 cgacgagctt gtagagctga cggcaggact gaggcgcaag gtggtgttcc atccggcagg
     1501 cctcgccgtc cgccgtttcg gcgtccatcc ggagcacctc gctgaagaaa tccatgaggc
     1561 attcgtgccg gctgtacacc ttgtccgcga tcgcctttcc gcgctcggtg aggtccacgt
     1621 acccgtactt ttcatagttg accaacccct gctcgctgag cttccccagg gcggcggtca
     1681 cgctgggcat cttcaccttg agcgatttgg cgatgtcctt gacgcggacg acccggtgcg
     1741 cccgcgagag cacggcgatg gtctccaggt agtcctccat attggaggag aggtccgtcg
     1801 attcgaacat gtcgcgcgcg tgatcgattt tcatgattgg ccgaatccct atccactatc
     1861 aagatacagt caagcaataa agttagttat ttctaatatt ttttcggaaa aatttcatct
     1921 cgggcatgct gaagtttttg tatttacaaa tccggttggc gcaccgagga ttcctccatc
     1981 atcgctaccg acaaattgca tccataatgg gccatacgat cttcatcacc ggcggctgcc
     2041 ggagcggaaa gagcacgcac gcgctcgcgc gcgcgcaggc gttgggcgca gagaccgtct
     2101 tcatcgccac ctgccggccc ggcgatgcgg agatggagcg gcgcatcgcg cgccatcgcg
     2161 cggagcgccc cgccgcatgg cgcacggtcg aggaatacgg cccgctggcc gacgtgctgc
     2221 ggtccgaaac gcagcgcgac agcgcaatca tactggattg cctgacgctc tatgtctcga
     2281 acctgctcgt atcggacatg ccgaccggcg ggatcatcga aaacatcagg gagatcgcgg
     2341 agatcatcgc gcagggcgcc gccaacgcga tcgtcgtatc gaacgaggtc ggttggggca
     2401 tcgtgccgga caacgccctc gcgcgcgact ttcgcgatat cgccggcacc gcaaaccaga
     2461 tcgttgcggc gcgcgcgaac gaggtcatct gcatgatcgc cggcattccg atgaggatca
     2521 aatgaaacca ggaaacacac acgagctggc cgacatcatc gccgcggtcc gtccgccacg
     2581 cgacacgctc atggcgcagg cgctggcgcg cctggacaat ctcaccaagc cgcgggggag
     2641 cctgggccgg ctcgaggaaa tcgccgtgcg catcgcctgc atcgccggac gcatcgatcc
     2701 gcagctcgcg aacaaggtcg tcttcaccat ggccgccgat cacggcgtgg ccgacgagaa
     2761 cgtgagcctg taccccaagg ccgtgaccga gcagatggtg tacaacttcc tccggggcgg
     2821 cgccgcggtg aacgtgctcg cgcgtcacgc gggcgcgcgc gtggtggtcg tggacatggg
     2881 cgtcgcggcc gatatcgccg acccgggcgg gacgctcgcg ataagaaaga tcgcctatgg
     2941 aaccgcctcg atgacaaagg ggcccgcgat gacccgcgac gaagcgattc gcgcgatcag
     3001 ggccggattc gaggtcgttg ccgaggagca tgcgcgccgt cccatcgaca tcgtcgcgtt
     3061 gggcgacatg ggcatcgcca acaccaccgc gtcgagcgcg atcgtgagct gcatcaccgc
     3121 gcttccgccc gaggagacca ccgggcgcgg caccggcatc gacgacgagc gcctcgcgtg
     3181 gaagatcggc gagatcgaga cggcgctcga tatcaatcgg cccgatcccg ccgaccccat
     3241 cgacgtcctg gcgaaggtgg gcggcttcga gatcggcgcc ctggcggggg tcgcgctggc
     3301 cgcggccgc
//
LOCUS       DAORHF010000357         3305 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_32639_length_3305_cov_6.134154, whole genome shotgun sequence.
ACCESSION   DAORHF010000357 DAORHF010000000
VERSION     DAORHF010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32639_length_3305_cov_6.134154"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="PKJ16_18270"
     CDS             complement(<1..893)
                     /locus_tag="PKJ16_18270"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HNV48994.1"
                     /translation="MARGLVNPSALEFTPEGDLLVTERPGRRRIIRKGVLLPDAVAGL
                     PPIQALGRGARKMSGLEQAGLRDVVLHPDFATNRLLYISYVKPGADSLGSLAVVRGRF
                     ERDRLTNVQEVFHARADGNGTSRASMWGGRLAFDKQRYLYITLGDRQWPSTGDLTAHP
                     AQRLSVHNGKTVRLHDDGRIPADNPFVNTPGALPETWTSGHRNAQGLAVHPVTGDVWS
                     NEHGPQGGDEVNLLRSGRNYGWPVVGYGVNYTTGRAIHEGTLREGMEPPAFVWVPSIG
                     VSGMIFYTGNAFPGWKGDMLVG"
     gene            874..1227
                     /locus_tag="PKJ16_18275"
     CDS             874..1227
                     /locus_tag="PKJ16_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48995.1"
                     /translation="MTSPRATSTTRKSWNAVWTTSAGSAASLGRAEVRAAQADAATVA
                     ARANEEERIIRGVLRRAGFEVGAVRVKIRWACDSSGNRIVGQLLRQRRVHDANIARVE
                     TDHASTDHGVSILML"
     gene            complement(1351..1776)
                     /locus_tag="PKJ16_18280"
     CDS             complement(1351..1776)
                     /locus_tag="PKJ16_18280"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HNV48996.1"
                     /translation="MIYTLDTNVLVDALRQPGELDRLKAFLSWALPSTVLSSVVVAEL
                     TAGARTAKAREALEHQFLAPFERRGRILAPSAAAWRRAATVTGATTAAPSANRFNDVL
                     LACQAREFGWTIVTRDADFGAIRRSVTGLKVAAPFPARP"
     gene            complement(1773..1985)
                     /locus_tag="PKJ16_18285"
     CDS             complement(1773..1985)
                     /locus_tag="PKJ16_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48997.1"
                     /translation="MARTRRVIRRKNMLIDQRKLDAAKAALGAETETAAVDAALDLVV
                     FRAEVFRGLDALVAAGGLGTRTRRAG"
BASE COUNT          541 a         1105 c         1141 g          518 t
ORIGIN      
        1 cccaccagca tgtcgccctt ccagccgggg aatgcattcc ccgtgtagaa gatcatcccg
       61 ctcacgccga tggacgggac ccagacgaac gccgggggct ccatcccctc gcgcaacgtc
      121 ccctcgtgga tggcgcgtcc ggtggtgtag ttcaccccgt agcccaccac cggccagccg
      181 tagttgcgcc ccgaacggag gaggttcacc tcatcgcccc cctgcgggcc gtgctcgttg
      241 ctccagacgt cgccggtcac gggatggacg gccagcccct gtgcgttgcg atgcccggag
      301 gtccaggtct cggggagcgc tccgggcgtg ttcacgaacg ggttatccgc ggggatgcgt
      361 ccgtcgtcgt gcaggcggac ggtcttgccg ttgtggacag aaagccgctg ggccgggtgc
      421 gcggtgaggt cgccggtgga gggccactgg cggtcgccga gcgtgatgta gaggtagcgc
      481 tgcttgtcga aggcgagccg accgccccac atggaggcac gcgaggtgcc gttgccgtcc
      541 gcgcgtgcgt ggaagacctc ctgcacgttc gtcaggcggt cacgctcgaa gcgcccgcgg
      601 accacggcca ggctgcctaa cgaatcggcg ccgggcttga cgtaactgat gtagagcagc
      661 cggttggtcg cgaagtccgg atgcaggacg acatcgcgca gcccggcctg ctcgagtccc
      721 gacatcttcc gcgcgcctcg gccgagtgcc tggatgggcg ggagccccgc gaccgcgtcc
      781 gggaggagga ctcccttgcg gatgatgcgg cgccgcccgg ggcgctcggt cacgagcagg
      841 tccccctccg gcgtgaactc gagggcggat gggttgacga gcccacgcgc cacctcgacg
      901 acgcggaagt cgtggaacgc ggtctggacg acgtcggccg ggagcgcggc gtcgttaggg
      961 cgcgcggagg tgcgggcggc gcaggccgac gcagcgacgg tagcggccag ggccaacgag
     1021 gaggagcgaa tcatccgggg agtgctccgt agggcgggat tcgaggtggg ggcggtgcgc
     1081 gtgaagataa gatgggcgtg cgacagttcg ggaaatcgga tcgtcgggca gcttctccga
     1141 caacgccgcg ttcacgatgc gaacatcgca cgcgttgaaa ccgatcacgc gagcacggat
     1201 catggggtca gcatcctgat gctttgatcc cgcgatcgcg agtttagcga tcgccgataa
     1261 actcgcgaat ctcgctataa ccggatgctg cgcccccgct gccatgcttc agtgtcacca
     1321 gaaaaagccg tcgcaccgcc cacgcctggc ctacgggcgc gcggggaacg gcgccgcgac
     1381 cttcagcccg gtgaccgaac ggcggatcgc gccgaagtct gcgtcccgtg tgacaatcgt
     1441 ccacccgaac tcgcgcgcct ggcatgcgag gagcacgtcg ttgaagcgat tggccgatgg
     1501 ggcggccgtg gtagcgccag tgacggtcgc cgctcgccgc cacgccgcgg cactcggcgc
     1561 gagaatgcgc ccgcgccgct cgaacggcgc caggaactga tgctcgagcg cctcgcgggc
     1621 cttcgcggtg cgcgcgccgg ccgtcagttc cgcgacgacg acgctcgaca gaacggtgga
     1681 aggcaacgcc cagctgagga acgccttcag gcggtcgagt tcgcccggtt gccgaagcgc
     1741 gtcgacgaga acgttggtgt cgagggtgta gatcaaccgg cgcggcgggt gcgcgtgccc
     1801 aatcccccgg ccgcgaccag cgcgtcgagg ccgcggaaga cttcggcacg aaagacgacg
     1861 aggtcgaggg cggcgtccac ggcggccgtc tcggtctcgg cgccgagcgc ggccttcgcg
     1921 gcatccagct ttcgctggtc gatgagcatg ttcttgcggc ggattacgcg gcgcgtcctg
     1981 gccatggctc ccttccggaa agtgccaagg gaaggtacgg ctccatacag ctcggagcaa
     2041 ggttcggtct gtatggcggt cgtcatgcac ccagtgagcg tcgccgtgag cgtccgcgag
     2101 gacgcgctcg acgagctgac ccgccagacg caggacatgg gactcggcca ctgacgggcc
     2161 cttcgctgtt gtctacgacg catgcgtgct gcaccccgcg ccgctgcggg acctgcgcta
     2221 tgcgccgaga ggaaaagccg tcgactcctc gaccgctccc atagcgcgcc gaatccatat
     2281 gcaagcgtcg ccataacatg gccctccacg actcccatgc tatgcgcagg ccatgacatg
     2341 gactccgccg gcccgctcaa ccgaaaccgt cagaaaccgt cagaaaggaa gaaggacgaa
     2401 gcgctccctc ccgctgagcg ctccgtaggg gatcgggcga acgccccgcc gattcacgcc
     2461 cgggcgaggt atctgtggga ccacgcgccg agcagatatg ggccatgtat tagcccctca
     2521 gcagatatcg gcccactatc tccccatcga cgtcctcagc agatcgtcgc tccgtgttcc
     2581 ttcccccgct ccccaccgtc tggaacctct acaacctcgc cagctccccc tacttccagg
     2641 ggacgctgga gcgccgcgaa cagtcgcagc gcccgctcgc gctcttcgtt tggcgcggcg
     2701 aggaactcca gcggttgcgg gcccgcgtgc acggctcacc cagggcgcgg tctcgaccgc
     2761 gctcgcctag ttcatcgaac gcggcttcgt cgtcgcgctc acgcgtcagg gagcagggcc
     2821 gacggagtac gtgctctccg ggaccagccg gctgatcttc ccggcggcgg ccaagccgtc
     2881 gcgcgtcagc gaggggccag caaaggggcg agctcggtcg tgacgcgcga aagcgggcct
     2941 gttcgaaagt ctgtttgtga gaccggtgat ggggtcacac gcgatcggtg aagcgcgcgc
     3001 cgaataggag cgcgaggact tgggaaatgg cgccgcgtca tctcgatctt cgggagcagg
     3061 atgtgtaggc ctcgatcgca tcccccacga cttgccgccg tcgcctgtgc gcctcgcatc
     3121 gtgccctcgc gacgcgacgc ggtcgttcga gcatcgcgca acgtgacttt gcgattcgac
     3181 acgtgcgttt gtgaatcgcg cagcgtgaca tggcggctcg gcgcggtcat ttgtgcatcg
     3241 tgcaacgaga ccatgcgatg cgacgcggtc gtttgtgcat cgcgcaacac gacctggcga
     3301 ctcgg
//
LOCUS       DAORHF010000358         3302 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_32691_length_3302_cov_4.881121, whole genome shotgun sequence.
ACCESSION   DAORHF010000358 DAORHF010000000
VERSION     DAORHF010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32691_length_3302_cov_4.881121"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..353
                     /locus_tag="PKJ16_18290"
     CDS             90..353
                     /locus_tag="PKJ16_18290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48998.1"
                     /translation="MKAIAGQLGALRGGSFSTIVWNRNTEGMKYRMTGSVEKSGDEMF
                     LFVKVVDLRTSSIVYVAKEKADSLDGIPDACRRVSQGVAERVR"
     gene            467..1459
                     /locus_tag="PKJ16_18295"
     CDS             467..1459
                     /locus_tag="PKJ16_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV48999.1"
                     /translation="MVIPVRLGASEASVDPTAERKRVAIISFNANNVPTAHANIVRNL
                     VEVALYSSNAFNLVEWEQVQKVMKSYGRDTIANTDMKSSLELGRLLATDYLVVGSVDK
                     IDEYRVTIRVVSIREKKIIIAYSRAYGSETGTEAVVEKMARDTARDLQKYIRTGSIEG
                     RFYDHHRILLGVEANYLYPAGRFYDLVNAGPGIALQGVIEHVFIDNLVMGVGASYSRL
                     SGKENANDSITIMSLSFPVSYRFYPQKRFFLGPIISPGLAFLTIAHGEGAGFNMRDNY
                     TDNFIEPICAGGGVIGVLPTDHLTVQFSAQYGFIYEREGVFQFYMYCLGVFFSF"
     gene            1601..3214
                     /locus_tag="PKJ16_18300"
     CDS             1601..3214
                     /locus_tag="PKJ16_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49000.1"
                     /translation="MKKYLVIAILLLMVHGISCTNSINDLFGSKGAASSSTVDPGVDS
                     AQFLYLGYLYGDSTGLAIHNQVLDGLDPADQSELQKALDASLRFPLPVDDPASGLPAA
                     FIPGTNWHRTRPASFADAANPEEIAEMLFDWFLSMEGIDGANGFCAAASGRISNLETY
                     SRTLVADDFVDPDKTSLDLSGIDLGDNAGMIAATNAGMQIQQCMLMIANVFFQQGGVG
                     ANPEVIDRLNQISFRRVTIQKGLKSIEKSVDQADAVASPDTRTDVNGARYFLEQLHTQ
                     LGACVHGSDIAAIVRSQMLSGGVYEDVVTRAYHAALASGERIAISRSAANLDVIARLD
                     KLKDDSAACDNQTAQCMAISFALCAGLGLPNCGEYATLICAAKQQECIANPPQAVVGS
                     VQYYFKEPLQYAQIALEDIALLRRLSLERLALLPYAIRGSDLLTVRADVARKDLQRLL
                     VIKEGIRGYWGNIDTYIANSTLDTTGADAYRLYDSMNQETEATDPTLHALMSAYVGTQ
                     DAYKRYQLAMYAVVLDWEAQLRAHIAAST"
BASE COUNT          716 a          882 c          981 g          723 t
ORIGIN      
        1 cccccgtgca accgccccgc ccagtatatg ccttggccat agaaccgttc accggtgagg
       61 gtgtcgatcg aggcgttcgg acagcggcga tgaaagcaat cgccggtcag ctgggcgcgc
      121 tcaggggagg ctccttctcg acgatcgtgt ggaaccgaaa caccgagggc atgaaatacc
      181 gcatgaccgg atccgtggag aagtcgggag acgagatgtt cctgttcgtg aaagtggtcg
      241 acttgcggac ctcttcaatc gtgtatgtcg cgaaggaaaa ggccgattcc cttgacggca
      301 tcccggacgc gtgccggcgc gtgtcgcagg gcgtggccga acgcgtgcgt tgagcgagtc
      361 gtgtgaccgc gcacggtgca aacacatgga cgggggagtt cgaatctcac acaaattgag
      421 tatggcatat cgcagggcga tcggtgcatg tatcgttttg tcgtgtgtgg tgatccccgt
      481 tcggctcggc gcatccgagg cctcagtcga cccgacggcg gaacggaagc gggtggccat
      541 catcagcttc aacgcgaaca acgtcccgac cgctcacgcg aacatcgtgc ggaatctcgt
      601 cgaggtcgcg ctgtacagct caaacgcgtt caacctggtc gaatgggaac aagtgcagaa
      661 ggtcatgaaa agctacggtc gggacacgat cgcgaacacc gatatgaaga gttcgctcga
      721 gctgggcagg ctcctggcca ccgactacct cgtggtcggc agcgtcgaca agattgacga
      781 ataccgcgtt accattcggg tggtctccat ccgggaaaaa aagatcatca tcgcctattc
      841 acgggcatac ggttcggaaa cggggaccga agcggtcgtg gagaagatgg cgcgtgacac
      901 tgcgcgcgac ttgcagaaat acatccgtac cggcagcatc gagggacggt tttacgacca
      961 ccaccggatt ctcctcggtg tcgaggcgaa ctacctgtat cccgcgggac ggttttacga
     1021 tctggtgaat gccggtccgg gaatcgcgct tcaaggagtg atcgaacacg tgtttattga
     1081 caacctcgtc atgggtgtgg gcgcgtcgta ttcccgcctg tcggggaagg agaacgccaa
     1141 cgattcgatc acgatcatgt cactgtcctt cccggtgtcc taccggtttt atccgcagaa
     1201 gcgttttttt cttgggccga ttatatcgcc cggcctggcc ttcctgacga ttgcacatgg
     1261 cgagggcgcc ggcttcaaca tgcgggataa ctataccgac aactttatcg agccgatatg
     1321 cgcgggcggc ggcgtcatcg gcgttctgcc gactgaccat ctgacggtac aattttccgc
     1381 acagtatgga ttcatctacg aacgggaggg tgtgttccaa ttctacatgt actgcctggg
     1441 cgtgtttttt tcgttttgaa atggtgatac ttctatgttg aaaggagtta ccacgcaatg
     1501 aatcaggaaa tccacgttgt ttgacatccg gcagaatgca aaactatcat acgcggatgt
     1561 cgccggctgg cgccggtgaa tagtaggtga ggtgtagacc atgaagaaat atcttgttat
     1621 cgctatccta ttgttgatgg ttcacgggat ttcgtgcacg aacagcatca acgatctgtt
     1681 tggctctaaa ggagcggcat catcctcgac cgtggatccg ggcgtcgatt cggcgcaatt
     1741 cctgtatttg gggtatctgt acggcgattc gacgggactg gcgatacaca accaggttct
     1801 ggacggtctt gatcccgccg accagtccga actgcagaag gcgctcgatg cgtcgttgcg
     1861 gtttccgttg ccggttgacg accctgcaag cgggctgccc gccgccttca ttcccggtac
     1921 gaactggcat cgaacgcgac cggcgtcgtt cgcggatgct gccaatcccg aagagatcgc
     1981 cgagatgctt ttcgactggt tcctgtcgat ggaggggatt gacggggcga acggcttctg
     2041 tgccgcggcc agcggccgca tttcgaacct tgagacctac tcgcgcaccc tggtggctga
     2101 tgacttcgtc gatccggaca agacatcgct cgatctgtcg ggaattgacc tcggggacaa
     2161 cgccggcatg atcgcggcaa cgaacgcggg tatgcagata cagcagtgca tgttgatgat
     2221 cgcaaatgtg tttttccaac aggggggtgt gggtgccaat cccgaggtga tcgatcgatt
     2281 gaaccagatc tcctttcgac gggtgaccat acagaaggga ttgaaatcaa ttgaaaaaag
     2341 cgtcgaccag gccgatgctg ttgcttcgcc agacacaaga accgatgtga acggggccag
     2401 gtattttctc gaacagctcc atacacagct cggtgcctgc gtacatggca gtgatatcgc
     2461 cgccatcgtt cgcagtcaga tgctctcggg cggcgtatat gaggatgtcg tgacccgtgc
     2521 atatcatgcg gcgttggcgt caggtgaacg gatcgccata tcccgcagcg ctgcaaacct
     2581 cgacgtcata gcccgattgg acaagttgaa ggatgatagc gcagcctgcg acaaccagac
     2641 agcgcagtgt atggccatca gctttgcgct ctgtgctggg ttgggattgc ccaattgcgg
     2701 ggaatacgca acattaatct gtgctgcgaa acagcaggag tgcattgcaa atcctccgca
     2761 ggcggttgtc ggatccgttc agtactactt caaggagccg cttcaatatg cccagattgc
     2821 tcttgaggat atcgccctgt tacgacggct cagtctcgag cgacttgctc ttcttccgta
     2881 tgccatccgt ggaagcgatc tgctcacagt cagagccgat gtcgcgagaa aagatctgca
     2941 gcgcctcctg gtcattaagg agggcatccg gggttattgg ggaaacatcg atacctatat
     3001 cgcgaacagc acccttgata ccaccggcgc cgatgcgtat cgactttacg attcaatgaa
     3061 tcaggagacc gaggcgactg atccgaccct gcacgccctg atgagtgcat atgtgggaac
     3121 ccaggatgca tacaagcgct accagctggc aatgtacgca gtcgtactcg actgggaggc
     3181 ccagctcagg gcgcatattg cagcgtccac gtagatagga accgttttat ggctggattg
     3241 agcgatgcgt gtccgataga ttgggacaaa tcgctcgatt ttcccatgcc cgtgcacgat
     3301 at
//
LOCUS       DAORHF010000359         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_32735_length_3299_cov_4.130703, whole genome shotgun sequence.
ACCESSION   DAORHF010000359 DAORHF010000000
VERSION     DAORHF010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32735_length_3299_cov_4.130703"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /gene="rplB"
                     /locus_tag="PKJ16_18305"
     CDS             complement(<1..323)
                     /gene="rplB"
                     /locus_tag="PKJ16_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270754.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HNV49001.1"
                     /translation="MGIKKFRPVTPTLRYKTVLTFDELTTDEPYKPLTVGKKESAGRG
                     HKGQVAVRRRGGGHKRRLRIVDYKMNKYGIEGTVSTIEYDPNRSANIALISYRDGEKR
                     YIVAP"
     gene            complement(355..639)
                     /gene="rplW"
                     /locus_tag="PKJ16_18310"
     CDS             complement(355..639)
                     /gene="rplW"
                     /locus_tag="PKJ16_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HNV49002.1"
                     /translation="MDYNDIIIKPVLSEKSTDLATQNKYVFRVATKANKTMVKKAIKE
                     IFNVNADKVNIICMRGKRKRARYQYGYTAAWKKAIVTLKQGDKLDIFETK"
     gene            complement(642..1268)
                     /gene="rplD"
                     /locus_tag="PKJ16_18315"
     CDS             complement(642..1268)
                     /gene="rplD"
                     /locus_tag="PKJ16_18315"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HNV49003.1"
                     /translation="MKIDKYSLDGKVIGQVDVPDSVFNAEINDVLIYELIKSANANLR
                     QGTHMTKQRAQIQGGGAKPWRQKGTGRARQGSIRAPQWRGGGTIFGPQPRDYRQDLPK
                     NMRRKAYISLFSLKAKEGAVKVVEEISITDGKTKAMAKIGISLGVEKGVLVTASDDSM
                     LKRAIRNLPWFAYNNVKRLSGRDIFYAKTLVITEGAVGALAELFSKGK"
     gene            complement(1279..1896)
                     /gene="rplC"
                     /locus_tag="PKJ16_18320"
     CDS             complement(1279..1896)
                     /gene="rplC"
                     /locus_tag="PKJ16_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HNV49004.1"
                     /translation="MKKSLIGRKMGMSQYIDPEGKVIPVTICEVGPCVVVQKKTVESD
                     GYEALKLGYLDTKEKRLAKPVLADLKKKNIAPKRVFCEVEVFDASLDVGSVVTCDIFG
                     ENDVVKVSGTSKGHGFAGVMRRHGFHGGRKTHGSNFHRAPGSIGACAYPSEVWKGKKM
                     PGRFGGDRVTVRNLKIVKVLKDKNIVLISGAIPGRKDSIITIKAV"
     gene            complement(2028..2345)
                     /gene="rpsJ"
                     /locus_tag="PKJ16_18325"
     CDS             complement(2028..2345)
                     /gene="rpsJ"
                     /locus_tag="PKJ16_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HNV49005.1"
                     /translation="MVNFTTQKIRVKLISFDAKLLDQSAAKIVATTKRSGAQVAGPVP
                     LPTKIEKFCVLRSPHVNKKSREQFEIRTHKRLIDIINPNEDTIEALMKLELPAGISVD
                     IKS"
     gene            complement(2489..>3299)
                     /locus_tag="PKJ16_18330"
     CDS             complement(2489..>3299)
                     /locus_tag="PKJ16_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HNV49006.1"
                     /translation="FLNKVDMIDASERAEMLELVEMEIRELLNKYEFPGDEIPIVPGS
                     ALKAMGCGCGKAECEACGPIINLAKALDEYIPTPQRATDKPFLMPIEDVFSITGRGTV
                     VTGRIERGIVHTGDEVEIVGFVETKKTVCTGVEMFRKILDEGMAGDNVGCLLRGTGKD
                     EVERGQVLAKPGSITPHKKFESEVYVLSKEEGGRHTPFFGNYRPQFYFRTTDVTGIIQ
                     LPSGVEMVMPGDNIKMTVELITPIAMEETLRFAIREGGRTVGAGVVAKIIE"
BASE COUNT          654 a          999 c          832 g          814 t
ORIGIN      
        1 tcgggagcga caatgtagcg cttctcgccg tcacgatacg aaatcagtgc gatgttcgcc
       61 gaacggttcg gatcatattc gattgtcgaa acggtcccct cgatgccata cttgttcatt
      121 ttgtaatcga caatgcgcaa gcgacgcttg tgtccgccgc cgcgccgccg caccgccacc
      181 tgccccttgt gcccgcgtcc cgcggactcc tttttgccaa ccgtgagggg cttgtagggc
      241 tcgtccgtgg tcagctcgtc gaaagtaagt accgttttat aacggagtgt cggcgtgacc
      301 ggtctgaatt tctttatgcc catggctcgt gcacctgatt cgtattgtcg tttgctactt
      361 cgtttcgaag atatcaagct tgtcgccctg cttgagggtg acgatggcct ttttccatgc
      421 cgcggtgtac ccgtactgat accgtgcccg cttgcgcttg ccgcgcatgc agatgatgtt
      481 caccttgtcg gcgttcacgt tgaagatctc cttgatggcc tttttcacca tggtcttgtt
      541 ggccttcgta gcgacccgga atacgtactt gttctgcgtt gcgagatcgg tcgacttctc
      601 ggacagcacg ggcttgatga taatatcgtt gtagtccatc gtcacttacc tttcgaaaac
      661 agttcggcga gcgccccaac cgcgccctcg gtgattacca aggtcttcgc atagaagatg
      721 tcccggcccg agagtcgctt tacgttatta taggcgaacc agggcagatt gcgaatcgcc
      781 cgtttgagca tcgaatcgtc ggaggcggtc acgaggacgc ccttctcgac acccagcgat
      841 attccgatct tcgccatcgc cttcgttttt ccgtcggtaa tggatatttc ctcgacgacc
      901 ttgaccgcgc cctccttcgc cttgagcgag aaaagggaaa tatacgcctt gcgacgcata
      961 ttctttggca gatcctggcg ataatcgcgc ggttgcggcc cgaaaatcgt tccgccgcca
     1021 cgccactggg gcgcccgaat ggatccctga cgggctcgac cggttccctt ttgtcgccac
     1081 ggcttcgcgc cgccgccttg aatctgtgcg cgctgctttg tcatgtgcgt tccctggcgc
     1141 aggttcgcat tggccgactt gatcaattca tagatgagaa cgtcgtttat ctcggcatta
     1201 aagacgctgt cgggaacgtc cacctgccca atcaccttgc cgtccagtga gtacttgtcg
     1261 atcttcatgg ccgttcgcct ataccgcctt aatggtaata atagaatcct ttctccccgg
     1321 aatcgcaccg gagatcagga cgatattttt atccttgagc acttttacga tttttaaatt
     1381 tcgcacggtc acgcgatcgc cgccgaatct tccgggcatc tttttccctt tccacacttc
     1441 gctgggatac gcacacgcgc caatggaacc gggcgcacgg tggaagttgg aaccgtgcgt
     1501 cttgcgaccg ccatgaaatc catgccgccg catcaccccg gcgaacccgt ggcccttcga
     1561 ggttccgctc accttcacca catcgttctc accaaaaata tcgcaggtga caacgctccc
     1621 gacatcgagg gaggcgtcga acacctcaac ctcgcaaaac acccgcttcg gagcgatgtt
     1681 tttctttttg aggtccgcca acaccggctt ggcaaggcgt ttttccttgg tgtcaagata
     1741 gccaagcttg agggcctcgt agccgtcaga ctcgacggtc ttcttctgga ccaccacgca
     1801 cggcccgacc tcgcatatcg taaccggtat caccttcccc tcggggtcga tatactggct
     1861 cattcccatt tttcttccga tcagagactt cttcatgact tgttcctgtt accgcccgta
     1921 cgcgcttttt acagaatact gcacagttcc actatttccg acagagtgga aatgccacgc
     1981 aaccaacccc gaacacgggg taaggggaat tccgaatcaa gccctcacta ggacttgatg
     2041 tccactgata taccggcggg aagctcgagc ttcatcagcg cctcaatggt atcctcattg
     2101 gggttgatga tatcaataag gcgcttgtgc gtcctgattt caaactgctc tcgagacttt
     2161 ttgttcacat gcggcgatcg caacacgcag aacttctcga ttttcgtcgg caacggaacc
     2221 gggccagcca cctgggcccc gctccgtttt gtcgtcgcta cgatttttgc cgccgattga
     2281 tccagcaatt tcgcatcaaa cgatatcagc ttaacccgta ttttttgcgt cgtaaaatta
     2341 accaactcag tagacctatc tcacatgaat tcgttgccgc atcccgcgta ctgccgaaac
     2401 gggctcccta gaaaccgtca gaccacaatc gctcgtggtc tgacaagaat cgtaaagggc
     2461 cttgtaaaat caagattttt tcaaaaaatt attcaatgat cttcgcgaca acgcccgcgc
     2521 cgaccgttct gccgccttcg cgtatcgcga agcggagggt ttcctccatc gcgatgggcg
     2581 taatcagctc gacggtcatc ttgatgttat ctcccggcat aaccatctca acgccggacg
     2641 ggagctgaat gatcccggtc acgtccgtcg tgcggaagta gaattgcgga cgataattcc
     2701 caaagaacgg ggtgtgacga ccgccctctt ccttggagag cacatacacc tcggactcga
     2761 atttcttgtg cggggtgatg gaacccggct tcgcgagcac ctggccgcgc tcgacctcgt
     2821 ccttgcccgt accgcgaagc aggcatccga cattgtcgcc agccatgccc tcgtcgagga
     2881 tcttgcggaa catctcgacg ccggtgcaca cggtcttctt cgtttcaaca aatccgacga
     2941 tctcgacttc gtcgccggta tgaacaatac cgcgctcgat acggccggtc accacggtcc
     3001 cgcggccggt gatcgagaac acgtcttcga ttggcatcag gaagggcttg tcggtcgcgc
     3061 gctggggcgt ggggatatat tcatcaagcg cctttgcaag attgatgatg ggcccgcacg
     3121 cctcgcactc cgccttaccg cagccgcagc ccatcgcctt cagggcggag ccgggaacga
     3181 tcggaatctc gtcgccgggg aattcgtact tgttcaggag ctcgcgaatt tccatctcga
     3241 cgagctcgag catttctgcg cgctccgagg cgtcaatcat gtcgacctta ttcaggaag
//
LOCUS       DAORHF010000360         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_33076_length_3277_cov_5.904097, whole genome shotgun sequence.
ACCESSION   DAORHF010000360 DAORHF010000000
VERSION     DAORHF010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33076_length_3277_cov_5.904097"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..105)
                     /locus_tag="PKJ16_18335"
     CDS             complement(<1..105)
                     /locus_tag="PKJ16_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014704564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family protein"
                     /protein_id="HNV49007.1"
                     /translation="MKALVPLAEGFEEIEAVSIIDVLRRADISVITAAL"
     gene            complement(201..737)
                     /gene="pth"
                     /locus_tag="PKJ16_18340"
     CDS             complement(201..737)
                     /gene="pth"
                     /locus_tag="PKJ16_18340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HNV49008.1"
                     /translation="MFIIACLGNPGSAYGRNRHNVGFLAGEYLARTNGIRLDKKSFSS
                     ITGKGRFAGSDVLIMLPQTYMNASGDAVQAAMSFYRLGGESLVVLHDEVELPFGEVRV
                     KFGGGHKGHNGVRSIIQQTGSADFHRIRIGVGRPPREGMSVADHALSNFSAEELAALE
                     GRYPAIAAMVDDVLNSAS"
     gene            complement(789..1130)
                     /locus_tag="PKJ16_18345"
     CDS             complement(789..1130)
                     /locus_tag="PKJ16_18345"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV49009.1"
                     /translation="MRITANDQKNYVVLKIEGSFSNEHIKELDFQVNLNAKKKRHIMI
                     DFTELSFISSSVLSALLLYNDQLRSENIVFNIFGLSENIRKMFDITGVSEHLFIFDSL
                     DDAAADIRGTA"
     gene            1310..2278
                     /locus_tag="PKJ16_18350"
     CDS             1310..2278
                     /locus_tag="PKJ16_18350"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV49010.1"
                     /translation="MVSPRTTLAIMITALTLATIAAPPETHGDPYNTNRPQSPTKADI
                     EKSAALYRQALSAYRTRKKPAEAVAIFKESAVLNPAMPTYYELGNALIDVKRYDEAIR
                     AYEAALALDTVASKTHLIYYNMACAESFANRIANALTNIEMALRNNYYHYAHLRSDAD
                     LANVRAAAKQTIDDLIARYDNRAHLSRLLVGRWEDAPMTAERANPSYRFNRDGTFSWA
                     AGNRSMTERAVSKSGRWSVRDGVLTLIVQERVIREGGRIIASTEPHAMGERTLVDYRE
                     KTVRPARAETSAWPISHVTPDGGELSPAKGRPTVLINTVRYWKTGD"
     gene            2324..>3277
                     /locus_tag="PKJ16_18355"
     CDS             2324..>3277
                     /locus_tag="PKJ16_18355"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HNV49011.1"
                     /translation="MRDSRARACGPSGNQLDMQNSTPLSAITPSPRRRSLWAPIVAVV
                     ALVALGLPSMLSAAAPFSVAPELYDHPLGAHIDILEDPGASLAIEEVCRAQPPAGTAW
                     RPVHSATHNFGFTRSAFWVRLAVANEYPRAMDWYMEIGYPMLDHIELYQSGPAGWEVR
                     RTGDQYPFAQRDIEYRNFMFALTEAPSSSRVLYLRFQSSSAMNIALAAWRPHDFVRQL
                     NIEHPALWMYYGLFLSLILYNLFMYYSVREHLYLYYILFLVSFFFCQLTLNGLAFQYL
                     WPARIWWANACLPLFMCSSYLWGALFSRRYLNLSYNWPFFDT"
BASE COUNT          649 a         1072 c          948 g          608 t
ORIGIN      
        1 cagcgcggcc gtaatcacgg aaatgtccgc gcgacgcaaa acgtcgatga tggaaacggc
       61 ctcgatctcc tcgaatccct cggccagggg caccagggcc ttcatgcgca cctccagaac
      121 ggcatcaacc gacgccgaat tcccgccacc cgttttgcga aatgggcgct cgtactttat
      181 atcggcatgc tggggggaat tcaggaagca gaattcaaga catcgtcgac catcgcggcg
      241 atggcgggat agcggccctc gagggccgcc agctcctcgg cgctgaagtt tgagagggcg
      301 tggtcggcca ccgacatccc ctcgcggggc ggacgcccca ccccgatgcg gatccggtgg
      361 aaatcggcgc tcccggtttg ctggatgatg gaccgaacgc cattgtggcc cttgtgcccg
      421 ccgccgaact ttacgcgcac ctccccgaag ggaagctcga cctcgtcgtg cagcaccacc
      481 agcgactccc cgcccaggcg gtaaaaggac atcgcagcct gcacggcgtc gccgctcgcg
      541 ttcatatagg tctgcggaag catgatgagc acgtcgctcc ccgcgaaacg acccttgccg
      601 gtgatcgacg agaatgactt tttatcgagc cgaatgccgt tggtgcgggc gagatattca
      661 ccggcgagga agccgacatt gtgacgattg cgcccatagg cgcttccggg attcccgaga
      721 caggcgatga taaacatgga aagcggctta cggacggcgc cgcggttcgc cggagcgccg
      781 caatcgcgtc atgcggtgcc tcgaatgtcg gcagccgcat catcgagcga atcaaagatg
      841 aacaggtgct cgctcacgcc ggtgatgtcg aacatcttgc ggatgttctc gctgaggccg
      901 aagatgttaa acacgatgtt ctcggagcgc agctgatcat tgtacaggag gagcgcgctt
      961 aacaccgaac tcgagatgaa cgacagctcg gtgaagtcga tcatgatgtg ccgcttcttt
     1021 ttggcgttca ggttcacctg aaaatcgagc tccttgatat gctcgttcga aaacgagccc
     1081 tcgattttca gcaccacata gtttttctgg tcgtttgctg taattctcat ccctgctcca
     1141 gagtttcgac caaaaaattc cgcatttccg ttattacatc cgtgcacgaa aaacctatcc
     1201 tgccggtcaa aatacgtcaa gataattttt gtggacaagc gccacgccga ccgccatact
     1261 atggacttgc attgcataca tcatagcaac ggctcacggg gggttgatga tggtttctcc
     1321 acgcaccaca ctcgcgatca tgataacggc tctgaccctg gcgacgatcg ccgcgccacc
     1381 ggaaacgcat ggagatccgt acaacacgaa ccgcccgcag agccccacga aagcggacat
     1441 cgagaaaagc gcggcgctct accgacaggc gctttccgcg taccggacgc ggaagaagcc
     1501 ggcggaggcc gtcgcgatct ttaaagagtc cgccgtcttg aatccggcga tgcccacata
     1561 ctacgagctc ggaaacgcgc tcatcgacgt gaagcgctac gacgaggcga tcagggccta
     1621 cgaggcggcg ctggccctcg acaccgtcgc gtcgaaaacg cacctcatct actacaacat
     1681 ggcctgcgcg gaatcgttcg cgaaccggat cgccaatgcg ctaaccaata tcgagatggc
     1741 gctgcgcaac aactattacc actacgcgca ccttcgaagc gacgccgacc tcgccaatgt
     1801 gcgcgcagcg gcgaagcaga cgatcgacga cctgatcgcg cgttacgaca accgcgcgca
     1861 cctctcacgc ctgctcgtcg gccgatggga ggacgcgccg atgacggccg agcgcgcgaa
     1921 cccgtcgtac cggttcaacc gcgacggcac attttcctgg gcggcgggga accggagcat
     1981 gaccgagcgc gcggtatcga aaagcgggcg atggagcgtg cgcgacggcg tcctcacgct
     2041 catcgtccag gagcgggtga tccgggaggg cggccggatc atcgcttcaa ccgagccgca
     2101 cgcgatgggc gagcgcacgc tggttgacta ccgcgagaag acggtacgcc cggcgcgcgc
     2161 cgagacaagc gcatggccga tctcgcacgt gactcccgat ggcggcgaac tgtcgcccgc
     2221 gaagggccgc cccaccgtcc tcatcaacac tgtgcgctac tggaaaacgg gcgactgatt
     2281 ttgtgattgc attatcgcga cggcggcgcc atcatgcgga caggtgcgcg acagccgggc
     2341 gcgcgcctgc ggtccgtccg gaaaccagct cgacatgcag aacagcacgc ccctgagcgc
     2401 catcacccca tcaccccgcc gccgttcgtt atgggcgccg atcgttgcag tggtcgcgct
     2461 cgtcgcgctc ggccttccgt cgatgctttc cgccgcagcg ccgttctccg tcgcccccga
     2521 gctctacgac catccgctcg gcgcgcacat cgacatcctc gaggaccccg gcgcgtcgct
     2581 cgcgatcgag gaggtctgcc gcgcgcagcc gccggcgggt accgcatggc ggccggtgca
     2641 cagcgcaacg cataatttcg gattcacgcg ttcggccttc tgggtgcgtc tcgccgttgc
     2701 gaacgaatac ccgcgcgcga tggactggta catggagatc ggctatccga tgttggacca
     2761 tatcgaactc tatcagtccg gacccgccgg ctgggaggtt cggcggaccg gcgaccaata
     2821 tcccttcgcg cagcgcgaca tcgaataccg caacttcatg ttcgcgctca ccgaggcgcc
     2881 gtcgtcctcg cgcgtgctct accttcggtt ccagagctcg agcgccatga acatcgcgct
     2941 cgcggcctgg cgtccgcacg acttcgtccg gcaattaaat atcgaacacc cggcgctgtg
     3001 gatgtactat ggactgttcc tgagcctgat cctctacaac ctgttcatgt actactcggt
     3061 gcgcgagcac ctctatctct attacatcct gtttctcgtg agcttcttct tctgccagct
     3121 cacgctcaac ggactggcct tccagtatct ctggcccgcg cgcatctggt gggccaacgc
     3181 ctgcctcccg ctgttcatgt gctcatcata cctgtggggg gcgctcttct cgaggcgcta
     3241 tctgaaccta tcgtacaact ggcccttctt cgacacc
//
LOCUS       DAORHF010000361         3257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_33387_length_3257_cov_5.018426, whole genome shotgun sequence.
ACCESSION   DAORHF010000361 DAORHF010000000
VERSION     DAORHF010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33387_length_3257_cov_5.018426"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PKJ16_18360"
     CDS             <1..417
                     /locus_tag="PKJ16_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49012.1"
                     /translation="CADDAPAPVGTGRILIMDDEEPIRTVAQRLLEKLGYEVQIAQNG
                     EEAIELFRAARQSGAPMDLAVLDLTVPGGMGGLECLHRLREIDPDVPAIVSSGYSNDP
                     IMAEHRRHGFDGCIVKPFRMQELSDAIRRLIRPARS"
     gene            558..2570
                     /locus_tag="PKJ16_18365"
     CDS             558..2570
                     /locus_tag="PKJ16_18365"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HNV49013.1"
                     /translation="MSYKPGVCTFCGTGCAHLLKTDNGSVDGVFPVQNHPVSRGRLCV
                     RGWNIHELLNTPERITTPLVKKDGALVPASYDEAIGVLVKGLADRKANAAEEIAVLAS
                     PRSSNEENYLLTRLARSVWGTNNITLDSDSGHRNSLDVLAAGTGMAGMTGSLEELRSC
                     EYVLVAGIDITKQNPIIGSEIHYAVRSGATLVTVDSRKTQIARLSTSFLQARPGSNKI
                     VYAAMAKILIEENLVDFDFLKKHNDGFEGFANSLGALRDEDISAKTGVSMDAIRAAAR
                     GLAAAKSAMVFFSSGISGLDADTISYIYNLFLIAGKVGRPGCGVNPIAGINNLQGGYD
                     MGVAPDLLSGFRPITDAKAASAVSAAWGAPISAKPGRGVHELLESGASKLTALIVVDH
                     DEGIVRHAERIKTIPFVAYIGAYKNPFMEYATVVLPIATYIEDDCTFTNTERRVQLSK
                     KKADAPSGVLPGWKLLSAIAEKSGSKWSYASSAAVMDEIAKITPTYAGISHAKLEGTF
                     GMQWPCDAKNPKGTARLSIEASKTFTFAPVSGSFEVPKPTGDFPSLLMIGKAQHFWHQ
                     NNIMRKTSIPLREYNTTLLLYPDGYVDITADEAKAAGVRDGWPVNVVSAKGSMRVQAR
                     VTDEVGPGAAYVPYFIQEMISEFLLENVDILKLGEDATIPVRIEKV"
     gene            2573..>3257
                     /locus_tag="PKJ16_18370"
     CDS             2573..>3257
                     /locus_tag="PKJ16_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49014.1"
                     /translation="MYLIKKSDVLRLAEELGKTHDLYGPFMEPATKQAMFERVGKAAD
                     IDLAAPIPALPPKHALLPHYEKILAYSYDKKTQKVDLAPIYDDRPKALFGVRPCDLTG
                     ILCLDRFFLGQEFLDEVYKYHRKNLFIVSQTCVAPFKQCFCVCCDGGPAASEGYDLNL
                     TDLGDTYLVEAGSEKGKKLAEAMGLAAADAKHQAEKTRRIDKCITLFDSVATDNKAWI
                     SRVMNRVTTG"
BASE COUNT          647 a         1079 c         1007 g          524 t
ORIGIN      
        1 tgcgcggacg acgcgccggc gcccgtcggc accggccgca tcctcatcat ggacgacgag
       61 gagcccatcc gcacggtcgc gcagcgtctg ctggaaaagc tgggatacga ggtgcagatc
      121 gcgcagaacg gcgaggaggc catcgagctc ttccgcgcgg cgcggcagtc cggcgctccc
      181 atggacctgg cggtcctcga ccttaccgtg ccgggcggca tgggagggct cgagtgcctg
      241 catcggctcc gcgagatcga cccggacgtg ccggcgatcg tgtcgagcgg ctattcgaac
      301 gatcccatca tggccgaaca ccggcggcac gggttcgacg gctgtatcgt caagcccttc
      361 cggatgcagg agctatcgga cgccattcgc aggctgattc gccccgcgcg gagctgaccg
      421 cgtccccgcg gatcaccgca acgacgtagg ggaatttccc tacgcgcaaa aagatttctc
      481 ccctatttac gcaccgcggg ttttttagta cctattgggt atcggaacgc gatcgctgat
      541 acccaaggag tacgtatatg tcctataagc ccggtgtatg caccttttgc ggcacaggct
      601 gtgcccacct cctgaaaaca gacaatggca gcgtcgatgg ggtgtttcct gtccaaaacc
      661 atcccgtgag caggggacgg ctctgcgtgc gcggttggaa catccacgag ctcctgaaca
      721 ccccggagcg catcaccacg ccgctcgtca aaaaggacgg cgccctcgtc ccggccagct
      781 acgacgaggc gatcggcgtc ctggtgaagg gcctcgccga tcgaaaggcg aacgccgccg
      841 aggagatcgc cgtgctcgcg tcgccgcggt catcgaacga agagaactac ctgctcaccc
      901 gcctggcccg cagcgtgtgg ggcaccaaca acatcaccct cgattccgat tccgggcacc
      961 gcaacagcct ggacgtcctt gccgcgggca cgggcatggc gggcatgacc ggctcgctcg
     1021 aggagcttcg ctcctgcgaa tacgtcctgg tcgcggggat cgacatcacc aagcaaaacc
     1081 ccatcatcgg cagtgaaatc cattacgcag tccgatccgg cgcgacgctg gtcacggtgg
     1141 acagccgaaa aacgcagatc gcgcgcctct ccacgagctt cctgcaggcg cggcccggct
     1201 cgaacaagat cgtgtacgcc gccatggcga agatccttat cgaggagaac ctcgtcgatt
     1261 tcgacttcct gaaaaagcac aacgacggct tcgagggctt cgccaactcg ctcggcgcgc
     1321 tcagggacga ggacatctcg gcgaagacgg gcgtgtcgat ggacgccatc cgcgcggcgg
     1381 cccgggggct tgcggccgcg aaatcggcca tggtgttctt ctcgtccggc atctccgggc
     1441 tcgatgcgga caccatcagc tacatctaca acctgttcct catcgccggg aaggtcgggc
     1501 ggccgggctg cggcgtaaac ccgatcgcgg gcatcaacaa tcttcagggc ggctacgaca
     1561 tgggcgtcgc gcccgacctg ctgtcggggt tccgccccat caccgacgcg aaggccgcga
     1621 gcgccgtgag cgcggcgtgg ggcgcgccca taagcgcgaa acccggccgg ggcgttcacg
     1681 agctcctcga aagcggtgca tcgaagctca cggccctgat cgtggtggac cacgacgagg
     1741 gaatcgtgcg ccacgccgag cggatcaaga cgatcccgtt cgtcgcctac atcggcgcgt
     1801 acaagaaccc cttcatggaa tacgcgaccg tggtgctgcc catcgccacc tacatcgagg
     1861 acgactgcac cttcacgaac accgagcgtc gcgtgcagct ttcaaaaaag aaggccgatg
     1921 cgccctccgg cgtgcttccg ggatggaaac tcttgtcggc catcgcggag aagtccggat
     1981 cgaaatggag ctacgcctcc tccgccgcgg tgatggacga gatcgcgaag ataacgccca
     2041 cctacgccgg gatctcgcac gcgaagctcg aggggacctt cggcatgcag tggccctgcg
     2101 acgcgaagaa cccgaagggc acggcgcgcc tgtcgatcga agcatcaaaa accttcacct
     2161 tcgcgccggt gagcggctcc ttcgaggtgc cgaagccgac gggcgatttt ccgagcctgc
     2221 tcatgatcgg caaggcgcag cacttctggc atcagaacaa catcatgcgc aagacgagca
     2281 ttccgctgcg cgagtacaac accaccctgc tgctctatcc cgacggctac gtggacatca
     2341 ccgcggacga ggccaaggcg gccggcgtgc gcgacggctg gcccgtgaac gtggtgtcgg
     2401 ccaagggctc catgcgcgtc caggcgcgcg tcaccgacga ggtggggccc ggcgcggcct
     2461 acgtgcccta ctttatccag gaaatgatct cggaattcct gcttgagaac gttgacatcc
     2521 taaagctggg agaggacgcg accatcccgg tgagaatcga gaaggtgtag ccatgtatct
     2581 gatcaagaag agcgacgtcc tccgtctggc tgaagaactc gggaagaccc acgatctcta
     2641 cggtccgttc atggagccgg ccacaaagca ggccatgttc gagcgcgtcg ggaaggccgc
     2701 ggacatcgac ctggccgcgc ccatcccggc gctcccgccg aagcacgcgc tgctgccgca
     2761 ttatgaaaag atcctggcct actcctacga caagaagaca cagaaggtcg atctcgcacc
     2821 gatctacgac gaccgcccca aggcgctctt cggtgtgcgg ccctgcgacc tcaccggcat
     2881 cctgtgcctg gaccgcttct tcctcggcca ggagttcctt gacgaggtgt acaagtatca
     2941 tcgcaagaac ctcttcatcg tctcgcagac ctgcgtcgcc cccttcaagc agtgcttctg
     3001 cgtgtgctgc gacggcgggc cggcggcctc cgagggatac gacctcaatc tcacagacct
     3061 gggcgacacc tatctcgtcg aggccgggag cgagaaggga aagaagctcg ccgaggcgat
     3121 gggcctcgcg gccgcggacg cgaaacacca ggccgagaag acgcgccgga tcgataaatg
     3181 catcaccctc ttcgactcgg tcgcgaccga caacaaggcc tggatctcgc gcgtgatgaa
     3241 ccgcgtgacc acgggct
//
LOCUS       DAORHF010000362         3209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_34157_length_3209_cov_4.600824, whole genome shotgun sequence.
ACCESSION   DAORHF010000362 DAORHF010000000
VERSION     DAORHF010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34157_length_3209_cov_4.600824"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..793
                     /locus_tag="PKJ16_18375"
     CDS             482..793
                     /locus_tag="PKJ16_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49015.1"
                     /translation="MKRLIVVLLVILATGFLTLCSHMDTVMKVVDQTTTPANVPANAS
                     VNGKYYTLLQVVRCPNDAGQYGQFYDYGAWSGSSWCNQTVQSGYWVWVNPNWYIWQNQ
                     R"
     gene            complement(1051..2637)
                     /locus_tag="PKJ16_18380"
     CDS             complement(1051..2637)
                     /locus_tag="PKJ16_18380"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HNV49016.1"
                     /translation="MNDKNKVIIFDTTLRDGEQSPGFSMNIEEKLHFANQLIRLGVDV
                     IEAGFPIASKGDFEAVRQIAEMARGVQIAGLARANNDDIDTAWEAIKNAESPRIHTFI
                     SSSDIHIEHQLKKSRAQVLEQAVRAVERAKSYTANVEFSPMDATRSDRAYLAEMVGAV
                     IAAGAVTVNIPDTVGYTVPSEFHDIIAYLMKNVKNIEQAVIAVHCHNDLGLAVANSLA
                     AVQAGARQIECTVNGIGERAGNTSMEEVVMAIRTRSELFGVHTTINTAQIAPTSRLLS
                     HITGITVQPNKAIVGANAFAHESGIHQDGLLKNAMTYEIMRPEDVGITKSTLVLGKHS
                     GRHAVVTRLADLGYQLSEEEIERFFKYFKALADVKKQIYDEDLEVLIGEAVYKIKGRY
                     KVTNVQISTGMFSPPMTMVTVKDRYRDGAESFEVAHGNGGVDAGINAVKKVTGTNAKI
                     DSFSLVAITGGSDALCEVTVNVSDEFEGHAISVFGNGVNIDISVAGILSFVDALNKLE
                     YKKKMNGFKDKAAAYAQAEL"
     gene            complement(3011..>3209)
                     /locus_tag="PKJ16_18385"
     CDS             complement(3011..>3209)
                     /locus_tag="PKJ16_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49017.1"
                     /translation="LLMKPEGAIIDCVYIRGGTELYPPPPEWGSANQPSSSKGRSVFR
                     IGPVDTDSAADWVSGLPTPGW"
BASE COUNT          621 a          898 c          924 g          766 t
ORIGIN      
        1 cttcatgatg gagcccttgc cgaattgctt ttcgatctgg aggatcgccg attcgagcgc
       61 ctgttccttt tggctgagtt gcttttgttc tgccatgata tatctcccac tgcgtgatgt
      121 gatcaaatgt gaacggaacc cgctgccgcg ccgcgcgccg gtgcggcctc cgcaatcata
      181 ttcgcgatgt gcactgcgcg cgtggcaatt atgtcacgtt agcggcacag agcgcaaaca
      241 ggaatttacc ggtattgaaa aaaatcatca tatacagaga atacgtatcc gtctccatgg
      301 aattgcatgt ttttgtattg aataggataa atcatctgaa ttttaaaagg acgacctgtc
      361 aagaaaaata ctataaattt gtttggtatt ttcaaatatt tttttacttg cgattggcgc
      421 ggtcggcagt agtataatgc tgtcgaaagc taacgcacta tcacacatac ggggtgatcc
      481 catgaaacga ttgattgtcg tgctgctcgt catccttgcg acgggatttc tgacgctgtg
      541 ttcgcacatg gacacggtca tgaaggtcgt cgatcaaacc acgacgccgg ccaatgtccc
      601 ggcaaatgcg agcgtcaatg gaaaatacta tacgctgctg caggtcgtgc gctgtcccaa
      661 tgacgcgggc cagtatgggc agttttacga ttacggggcg tggagcggca gctcgtggtg
      721 caaccaaacc gttcaaagcg ggtactgggt ctgggtgaac ccgaactggt atatctggca
      781 aaaccagcgc taaggcgaga tggtccaacg gtattggtgg ctgttccgtc gggctcggaa
      841 ccgccaccga tcgttgcctg cgggttatga tgcgctgtcg cggggccgga tattcggttc
      901 cgcgacgcgc ctatcatact gcgatgcgag tgtagtacgg tttggacggc ggatcggaca
      961 acaccgttcg tgtcttccga tgatgatcga acaaacaaaa cagcaagcct catggcgggg
     1021 cgtgctgttt catgagggtc gtgttcgttt ctacagttcc gcttgggcgt aggcggccgc
     1081 cttgtccttg aatccgttca tcttcttttt gtattcaagc ttgttgagcg cgtccacgaa
     1141 cgacagaatg ccggccacgg atatgtcgat attgacgccg tttccgaaca cgctgatcgc
     1201 atgcccctcg aactcgtcgc tcacgttcac cgtcacctcg cagagagcgt cggaaccgcc
     1261 ggtgatcgcg accaggctga aggagtcgat cttcgcattg gtgcccgtga ccttcttcac
     1321 ggcgtttatc ccagcgtcca cgccgccgtt cccgtgcgcg acctcgaagc tctccgcacc
     1381 gtcgcggtag cggtccttga cggtcaccat ggtcatgggc ggggagaaca tgccggtcga
     1441 gatctgtacg ttcgtgacct tgtagcgtcc ttttatcttg tacaccgcct cgccgatcaa
     1501 gacctcgagg tcctcgtcgt agatctgctt cttgacgtcg gcgagcgcct tgaagtactt
     1561 aaagaaccgt tcgatctcct cctcggaaag ctgatagccg aggtccgcga gccgggtgac
     1621 cacggcgtgg cgtcccgagt gcttgccgag cacgagcgtg gatttggtga tgcccacgtc
     1681 ctccggccgc atgatctcgt aggtcatcgc gttcttgagc aggccgtcct ggtggatgcc
     1741 cgactcgtgc gcgaaggcgt tggcgcccac gatggccttg tttggctgca cggtgattcc
     1801 cgttatgtgc gagaggagcc tgctcgtggg cgcgatttgc gcggtgttga tcgtggtgtg
     1861 cacgccgaag agttcgctgc gagtgcggat cgccatcacc acctcctcca tggaggtgtt
     1921 gcccgcccgc tccccgatgc cgttcacggt gcactcgatc tgtcgcgcgc ccgcctgcac
     1981 cgccgcgagt gagttggcca ctgcgagccc gaggtcgttg tggcaatgca ccgcgatgac
     2041 ggcctgctcg atgtttttca cgttcttcat cagataggcg atgatgtcat ggaattcgga
     2101 cgggacggtg tagccgacgg tgtccggaat gttcacggtc acggccccgg cggctatgac
     2161 ggcccccacc atctcggcaa ggtacgcccg gtcgctgcgc gtggcgtcca tgggggagaa
     2221 ctcgacgttc gcggtgtatg atttcgcgcg ctccaccgca cgcaccgcct gttcgagcac
     2281 ctgcgcgcgc gattttttca gctggtgctc gatgtggatg tcggagctgg agatgaaggt
     2341 gtggatgcgc gggctctccg cgttcttgat cgcctcccac gcggtgtcga tgtcgtcgtt
     2401 gttcgcccgc gcgagccccg cgatctgcac gccccgcgcc atctcggcga tctggcgcac
     2461 ggcctcgaaa tcgcctttgg aggcgatggg aaagcccgcc tcgatcacgt ccacccccaa
     2521 tcggatgagt tggtttgcga agtggagctt ctcctcgatg ttcatgctga agccgggcga
     2581 ctgctcgccg tcacggaggg tcgtatcgaa aataatgacc ttgtttttat cgttcatgat
     2641 gatttccttt tcattataag atagtatcgg catccccgca gtgcgggcgt gcgtaattcg
     2701 cacaaatgaa aatcatggaa tcatcaaagc gtatacggga atatcatcat ccccttcacc
     2761 tccgggtgtc ccataaaata aaaaggccgt cagggaggtt tccgtgacgg cctttgcaag
     2821 ttcagcgcct aagcgtatat cacgaacccc tccgggtatc tggcaacccg agcagcagca
     2881 gtaggccgaa aagtagggcg aggatgcgtg acattggttt ctcctggcga ttgattgcat
     2941 ccaataaacc tcatcgggtg aggttgtcaa tattatttac gaccgaaatg ctttaaaata
     3001 cgcacttttt tcaccacccc ggcgtgggca atccgcttac ccaatccgcc gctgaatcgg
     3061 tatcgacagg acctatccgg aacaccgacc ttcctttcga cgatgacggc tggttggcgg
     3121 aaccccattc gggcggtgga ggatagagct ccgtcccgcc ccggatgtac acgcagtcga
     3181 tgatcgcgcc ctcgggtttc atgagcaat
//
LOCUS       DAORHF010000363         3184 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_34532_length_3184_cov_4.041227, whole genome shotgun sequence.
ACCESSION   DAORHF010000363 DAORHF010000000
VERSION     DAORHF010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34532_length_3184_cov_4.041227"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PKJ16_18390"
     CDS             <1..576
                     /locus_tag="PKJ16_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HNV49018.1"
                     /translation="TVFELQSLFLALALVGFFSYATFSGLMVPLLAAAACGIGLAAVV
                     YVRSLRRDEGPDAAAAGAEPGGGSSIRSHANPLDLESALVFAVLFVIVKTASHFAILH
                     VGTSGFYALSFVMGFTDVDPFILSVVQSPHEIATHMAVFAIILAAMSNNLLKGLYAYL
                     FTRSAMGLHSLITLFVMSLMSIAAYALMARW"
     gene            627..700
                     /locus_tag="PKJ16_18395"
     tRNA            627..700
                     /locus_tag="PKJ16_18395"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:661..663,aa:Pro,seq:tgg)
     gene            705..778
                     /locus_tag="PKJ16_18400"
     tRNA            705..778
                     /locus_tag="PKJ16_18400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:739..741,aa:Arg,seq:tct)
     gene            1005..2009
                     /locus_tag="PKJ16_18405"
     CDS             1005..2009
                     /locus_tag="PKJ16_18405"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HNV49019.1"
                     /translation="MAYEPRYTISPRLLSLVEAIAALRERIQGAAVELTWLPAMQKDT
                     RTRNTHASTAIEGNPLTLEEVRAIEEGRDFFADPRSKREVVNYLAGLRYVEKHVSKKR
                     IRHEDVFALHRILAEGVMDQGRAGRYRDIPVRVGAYVPPAPGEVSGLMFEFLDWWNHQ
                     STSISPVISSAIVHYRFEAIHPFADGNGRVGRALALWELYRRGFDTHHIFSVDEFYWD
                     DRPRYYSALESVRHEGDDLTSWLEYSAEGLRQTLDRVWLRVQSFSNLTKEKIVLRPKQ
                     ERLLALLRDIGSMSPAEIWVALDISKQGAMDLIKPLVEAGLIEKVGGKKTGRYQLRKP
                     "
     gene            complement(2345..>3184)
                     /locus_tag="PKJ16_18410"
     CDS             complement(2345..>3184)
                     /locus_tag="PKJ16_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49020.1"
                     /translation="SSCSSKSGGDGGGGSGGGVGASVPSRVYRINYSSGVVVGFNFRS
                     LFVQNAWAAELQAYKVAEFDADTGKLKKVILYNGSGLSDSYYDYELNADGRPTRHYCR
                     NTDGTLWYQYHMTWNAEKKITEFYSKKANGTTDTYIEFLYLPDGNLEWLKQWNDNTRV
                     TLYYRLKNVYDTDGNLIRVYAGTASGIERFEVTYPESGKRRIDIFQHATMDDPTSGDG
                     SNYRVFTYDEKNRLVHILFGDSNTERVYEYAKESVSEDKSDEWETWYNLPVNLQAWID
                     GPQ"
BASE COUNT          685 a          865 c          867 g          767 t
ORIGIN      
        1 accgtgttcg aactgcagag cctgttcctc gcactggcgc tggtgggctt tttcagctat
       61 gcgacctttt ccgggctcat ggttcccctg ctggcggcgg ccgcgtgcgg gatcggcctg
      121 gccgcggtcg tctatgtgcg cagcctgcgg cgcgatgagg ggcccgatgc ggcggccgcg
      181 ggagcggagc cgggcggcgg atcgtcgata cgcagccacg cgaacccgct cgatcttgaa
      241 tcggcgctgg tcttcgccgt cctgttcgtg atcgtgaaaa cggcgagcca tttcgccatc
      301 ctccatgtgg ggacctccgg gttttacgcg ctatcattcg tcatgggttt cacggacgtg
      361 gacccgttca ttctcagcgt ggtccagtct ccgcacgaga tcgccacgca catggcggtg
      421 ttcgccatca tcctggcggc catgtccaac aatctgctca aggggctcta tgcgtatctt
      481 ttcacgcgaa gcgcgatggg gttgcacagc ctcatcaccc tgtttgtcat gagtttgatg
      541 agcatcgccg cgtatgcgct catggcccgg tggtgaaaat agtattgaca cgccctccgc
      601 gcgccggtac gtgacaactg aaatcgcggg gtgtggcgca gcccggtagc gcacctggtt
      661 tgggaccagg ccgtcggagg ttcaaatcct ctcaccccga atacgcgcca gtagctcagt
      721 cggatagagc aacagccttc taagctgtgg gtcacaggtt cgattcctgt ctggcgcgca
      781 ggtgctcccg agagatcggg ggcatgttgt ttccgtccat agcgtttccc tctgtcacaa
      841 caaagcgtgc catcctccac gaactgaaga aatagctctc gcgctgtggc gtttgtatgg
      901 cgccgtgatg tcttttcacg aatagcgggt gtagccggtt cgcaggagca tggaagacct
      961 gaaatctact tgactgataa cttgacttat acttgactaa tcacatggcg tacgaacccc
     1021 gatatacgat cagcccgcgc ctcctatccc tagtggaggc cattgcggcc cttcgtgagc
     1081 gaatccaggg cgccgccgtc gagcttacat ggctgccggc catgcagaag gacacgcgca
     1141 cgcgcaacac gcatgcctcg actgccatcg agggcaatcc tctcacgctc gaagaagtgc
     1201 gggccatcga ggagggcagg gatttcttcg ccgatccgcg atcgaagcgc gaggttgtga
     1261 actaccttgc aggattgcga tatgtggaga agcacgtaag taagaagaga atccggcatg
     1321 aggacgtgtt cgcgctgcac cgcatcctgg ccgagggggt gatggatcag ggcagggccg
     1381 gccgttaccg ggacatcccg gtcagggttg gagcgtatgt tccgccggcg ccgggcgaag
     1441 tatcgggatt gatgtttgaa tttcttgatt ggtggaatca tcaatcgacg tcgatatctc
     1501 cggtcatcag ctccgctatc gttcattacc ggtttgaggc aattcacccc tttgccgacg
     1561 gcaacggacg cgtcggccgt gcgctcgcgc tttgggaact gtaccgccgc gggttcgata
     1621 cgcatcacat cttctccgtc gatgagttct actgggatga ccggccgcgc tactactccg
     1681 cactcgagtc cgtccgtcat gaaggggacg atctgacatc ctggctcgag tattcggccg
     1741 aggggcttcg acaaaccctc gatcgagtct ggctgcgggt ccagtcattc agtaatctca
     1801 caaaagagaa gattgtcctc aggccgaaac aggagcggct gctggcactt cttcgggata
     1861 tcggcagcat gtcgcccgct gagatctggg tcgcattgga tatatccaaa caaggagcga
     1921 tggatctgat taagccgctc gtcgaagccg gcctcatcga aaaggtcggc ggtaagaaga
     1981 cgggtcgtta tcagctacgc aaaccatagc tgatagcatt atagtgtgct ataggacctc
     2041 tgaatggggt ttggcggcag gatcggttgt tatacagccc ctatccattc ggccggatgt
     2101 cgttgcaatg gcggtcatat cgaatctgtt aatgagaagt gtatgtgttt gttggttcga
     2161 gtttagatcg gttattgcgg taaatagaat caatctcgac cttgcgtatg gatatatcgt
     2221 gcaagctaac gcaggcatac agttgcaggt ataattgcaa accgcatgcc tgcgttcgaa
     2281 ggatggttac ttgatctgga atacacacat aaccagaaga acaaggagac aaacgagaat
     2341 gagcttattg cgggccgtca atccatgcct gcagatttac gggaagatta taccaagtct
     2401 cccattcgtc tgatttatct tcagagactg attcttttgc atactcatat actcgttctg
     2461 tgtttgagtc gccaaacaaa atatggacga gcctgttctt ttcatcgtag gtaaatacgc
     2521 gatagttcga cccatcaccc gacgtgggat cgtccattgt cgcatgctga aagatgtcga
     2581 ttcgacgttt ccccgattca ggataggtaa cctcgaagcg ctcgataccg gaggccgtac
     2641 ccgcatatac gcgaatgaga ttaccgtcag tatcgtacac gttcttcaga cggtaataca
     2701 gggtaaccct cgtattgtca ttccactgct ttaaccactc aaggttgcca tcaggaagat
     2761 agagaaactc gatatatgta tccgtcgtgc cattggcctt tttcgagtag aactcggtga
     2821 tcttcttttc ggcgttccac gtcatatgat actggtacca cagcgttcca tccgtatttc
     2881 tgcagtaatg tcgtgtaggc cgcccatctg cattgagttc ataatcatag tagctatccg
     2941 aaagtccgct accattgtac aaaattacct ttttcagctt ccctgtatct gcgtcaaact
     3001 cagctacctt atatgcttgg agttctgcag cccatgcgtt ttgtacgaac agactacgga
     3061 aattgaaacc aacaaccaca ccggagctat aattaattct atacacgcga cttggcaccg
     3121 aggcgcccac tccaccgcca ctgccaccac caccatcacc gccacttttg cttgaacaac
     3181 ttga
//
LOCUS       DAORHF010000364         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_34610_length_3180_cov_4.034240, whole genome shotgun sequence.
ACCESSION   DAORHF010000364 DAORHF010000000
VERSION     DAORHF010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34610_length_3180_cov_4.034240"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="PKJ16_18415"
     CDS             complement(<1..992)
                     /locus_tag="PKJ16_18415"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HNV49021.1"
                     /translation="MKLLVGFSAPAIVGTIVTSMYSVIDRIFVGLVVGPLAIAGMGLT
                     FPISLVLMAFGMLIGTGTAARVSISLGQKDLASAERILGNGISLVLIASLSLTVSGLI
                     FLDPILSLFGGSPATLPYARQFIGIILLGAVFQFLGFGLNGVISAQGHPRIAMMTMIL
                     NAALNIVLLILFIYGFGWGVRGSALATLIAQATSAAWVMGFLRSRRSMLRIRLRNMRL
                     DRRIVLAIASVGMAPFAMQFTGSFVNAILNRGLKEHGGDMAVGAMSAIFSLAILLVMP
                     VIGINHGMQPIVGYNFGAQKYDRVKKTLTLAIAGATIHLSAMFALVQIGPEFFIR"
     gene            1305..2669
                     /locus_tag="PKJ16_18420"
     CDS             1305..2669
                     /locus_tag="PKJ16_18420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HNV49022.1"
                     /translation="MAQREKDYDLLVIGGDAAGMSAASQARRIDPAMTIGVFDKGEHI
                     SYAACGMPYYIAGQIADSNELIAVDRDEFVAKRNVDITNGAEAVRVDFDAREVVIRVG
                     SEERAYGFRKLVIATGARAFRPPLRGIDAPDVFFLRNLSDGIAIKTRLAEKRPKRIVT
                     IGGGFIGLELAEAFRGLGIETVIVERMESVAMSMSPFIRERIITTLQKNGVTLVTGAE
                     INAIVEAGGALGVVTGAGTFDADMVVISTGVVPNTEFLIASPLAFDSRKAIVVNGKSE
                     TNLPGVYAAGDCATVPHLVTGKDVYMPLGSTANKQGRVAGLQVAGVASEVFPGIVGSQ
                     FVKIFDLEVGKTGFNAADAEREGVPAESVTATWRSRAGYCPKAQKIFMTLTINADTRH
                     VIGAELAGTDGAALRTNVVAAAIAARMTIDELAYLDLGYAPPFSPVWDPVNAAAQKFV
                     KRGM"
     gene            2676..>3180
                     /locus_tag="PKJ16_18425"
     CDS             2676..>3180
                     /locus_tag="PKJ16_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49023.1"
                     /translation="MKYTPGFFDRLDCDREEKVQCVEIIDALIVLSEKSRRMGLLVLE
                     DDIGLIDDAFLKKGLQLIVDGTDPEIVRNVLYTRMLASRLTGSALLRAVLILEGIMGI
                     QAGDNPGILADKLSAFLGADVDLWDEHWARVMRESEAMDAGPDEYCPDDMPAHDDDGP
                     ELPHGEIR"
BASE COUNT          638 a          907 c         1066 g          569 t
ORIGIN      
        1 cgaatgaaga actccgggcc gatctggact agcgcgaaca tggccgaaag atgaatcgtt
       61 gcgcccgcga tcgcaagcgt gagcgttttc tttacccggt cgtatttttg cgcgccgaaa
      121 ttatatccga cgatcggctg catgccgtga ttgatgccga tgaccggcat gacgagcagg
      181 atcgcgagcg agaagatggc gctcatggcc ccaacggcca tgtcgccgcc gtgctccttg
      241 agcccgcgat tcaggatggc gttcacaaag ctccccgtga actgcattgc gaacggcgcc
      301 atgccgacgg aggcgatggc gagaacgatc cgtcgatcaa ggcgcatgtt tcgcagtctg
      361 atccggagca tgctgcgcct gctccgcaaa aagcccatta cccacgccgc cgaagtcgcc
      421 tgcgcgatga gggtggccag cgccgatccc cgcacgcccc agccaaaccc gtaaataaac
      481 aggatcagga ggacgatgtt gagcgcggcg ttcaggatca tcgtcatcat ggcgatgcgc
      541 ggatgcccct gggcgctgat gacgccgttg aggccgaagc cgaggaactg gaacacggcg
      601 cccagcagga tgatgccgat gaactggcgc gcatagggga gcgttgccgg gctcccgccg
      661 aagagcgaca ggatggggtc cagaaagatg aggcccgaga ccgtgagcga cagcgatgcg
      721 atgagcacca gggagatgcc attgccaagt attcgttcgg cgcttgcgag gtctttttgt
      781 cccaggctga tggagacgcg cgccgcggtg ccggtgccga tgagcatccc gaaggccatg
      841 aggaccaggg agatggggaa ggtgagtccc atgccggcga ttgcgagcgg ccccaccacc
      901 agccccacga agatgcggtc gatgacgctg tacatcgagg tcacgatggt gcccacgatc
      961 gcgggggccg agaagccgac caggagcttc aaaatgggtt cttcgccgag gcgttgggtg
     1021 ttacgttcca tagatgggat gatggtatct gttgtctccg aggcgacatg cttcccctgg
     1081 acatcgcatt gtcaagtgga gaaaatcgtt cgaaccgtca tgatttgttg tcagatattc
     1141 atgcccggct ggtataacag tactgatcgc tttgctcgcg aagatattga gttatgcaac
     1201 aatactacgg ttggggacaa ttcgagtata aaaaaaactt gcgggatgcg ccggaatgtg
     1261 cgatacgatg tcggtgtagc ggactgagga atgctggggg gaccatggcg cagagagaga
     1321 aggactatga cctgctggtc atcggcggcg acgcggccgg gatgagcgcg gcgtcgcagg
     1381 cacggcgcat cgatccggcg atgacgatcg gcgtcttcga caagggcgag cacatttcct
     1441 acgccgcctg cgggatgccg tactacatcg cggggcagat cgcggattcg aacgaactca
     1501 ttgccgtgga ccgcgacgag ttcgtcgcca agcgcaatgt cgatataacc aacggcgccg
     1561 aggcggtgcg ggtcgatttc gacgcgcgcg aggtggttat ccgcgtcggc tcagaggaac
     1621 gggcatacgg cttccggaag ctggtgatcg ccaccggcgc gcgcgccttc cggccgccgc
     1681 tgcgcgggat cgacgcgccg gacgtgtttt tcctgcgcaa cctgagcgac ggcatcgcca
     1741 tcaagacccg attggcggag aagcggccga agcgcatcgt gaccatcggc ggcggcttca
     1801 tcgggctcga gctcgcagag gcctttcgcg gcctgggcat cgagacggtc atcgtcgagc
     1861 ggatggaatc ggtggcgatg agcatgtcgc cgtttatccg cgagcggatc atcacgacgt
     1921 tacagaaaaa cggtgtgacg ctcgtgaccg gagcggagat caacgccatc gtggaggcgg
     1981 gcggcgcgct tggcgtggta accggtgcgg gaaccttcga cgccgatatg gtcgtgatat
     2041 cgacaggcgt agttccaaac accgagtttt tgatcgcctc gccgctggcc ttcgattcgc
     2101 gaaaggcgat cgtggtgaac gggaaatcgg agacgaacct ccccggcgtt tatgcggccg
     2161 gtgactgcgc caccgtgccc cacctggtca ccggaaaaga cgtctacatg ccgctgggat
     2221 cgaccgcgaa caagcagggg cgcgtggcgg ggctgcaggt cgcgggtgtc gcctccgagg
     2281 ttttcccggg catcgtgggc tcgcagttcg tgaagatctt cgatctcgag gtgggaaaga
     2341 ccggcttcaa tgcggccgat gcggagcggg agggcgtccc ggccgaatcg gtcacggcca
     2401 cctggcgatc gcgcgcgggc tactgcccga aggcgcagaa gatattcatg acgctcacca
     2461 tcaatgcgga tacgcgccac gtcatcggcg cggagctcgc cgggaccgac ggagcggcgc
     2521 ttcgcacgaa cgtggtcgcc gccgccatcg ccgcacgcat gacgatcgac gagctcgcct
     2581 atctcgatct cggctatgcg ccgccctttt cgccggtgtg ggaccccgtg aacgcggccg
     2641 cgcaaaaatt tgtaaagcgg ggaatgtgag ggaccatgaa atacactcca ggattttttg
     2701 accgtcttga ttgcgatcgc gaggagaagg tgcaatgcgt cgagatcatc gacgccctca
     2761 ttgtgctgtc ggaaaagtca cggcggatgg gccttctcgt cctcgaggac gacatcggac
     2821 tgatcgacga cgctttcctg aagaagggac tgcagcttat cgtggacggc accgacccgg
     2881 agatcgtcag gaacgtcctg tatacgcgga tgctggcgag ccgcctcacg ggcagtgcgc
     2941 ttctacgcgc ggtccttata ctggaaggga tcatgggtat tcaggcgggc gacaatcccg
     3001 ggattctcgc cgacaagctc tccgcctttc tgggcgcgga cgtcgatctc tgggacgagc
     3061 actgggcgcg cgtcatgcgg gagagtgaag cgatggacgc gggccccgac gaatattgtc
     3121 ccgacgacat gcccgcccac gacgacgatg gcccggaact gccgcatggg gagattcgtg
//
LOCUS       DAORHF010000365         3164 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_34875_length_3164_cov_4.054358, whole genome shotgun sequence.
ACCESSION   DAORHF010000365 DAORHF010000000
VERSION     DAORHF010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34875_length_3164_cov_4.054358"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PKJ16_18430"
     CDS             complement(<1..674)
                     /locus_tag="PKJ16_18430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49024.1"
                     /translation="MIPTKTRFSLLLAIALLAWAIVAPAPPVSGQSSTDGVRIDWEAI
                     DGAIGYAIRITDKDGKEIIATSTETNTVTVPLPPGTYRLRIGVVNKFRKIGSWSEWET
                     IEIKRGTTAIETRFSRNLFNLGIKLSVGIVNYSLSGNWQNYFMDSIGNFNGILAYDVG
                     THGAFGEWGVLRYTGVEFEGSTMEFKAKQNTAGTTFDIQGSTFGVNLYAATRFDIPVN
                     FILKAGG"
     gene            complement(679..2811)
                     /locus_tag="PKJ16_18435"
     CDS             complement(679..2811)
                     /locus_tag="PKJ16_18435"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HNV49025.1"
                     /translation="MKLSRNDKTVLVAGLVIIVLFSGLFYYDLTRRRGAGSEEVVGTI
                     TFKRQQAQRKYASQVVWEDVAEAVPVHNHDSIRTAELSEAVIKLKDGTEIALNENSMI
                     LISFSKNEVDIEFAQGSINAKRGDADPKQKMNIKSKDTVVSIDKSDVSLTRMGGTDLN
                     LTVNRGTAKINRGETEKLIRQDEKAVLSKDALTIYRLNLKLLSPTPNEHITTTAARHR
                     VQFSWEAVRDAADIHHEVAQSPSFGDIVSIRRIAGDGRTLELPSGIYYWRLRAMNRKT
                     RKIEYSEVRRLSITRDAPLSLISPHDGATYSYNAKKPLVSFKWSGSELSSSYTLTVAS
                     DSSMSRVVQRIDTSATGAALDTLGSGTYYCMVRRSGAGQSVSSPVHRFTITQTASLDP
                     PELVFPPDARQFNKISLKNGTITFTWRTVPEIGSSRIDIARDRGFTNIIHTGTSATNF
                     AKLTGALDTGPYFWRVTGMIRGGSESVTSSARQFRIIESDVIAIIAPRDNATIAPDGE
                     DTAAAVAFSWKRTEMPGQFHLVVSRDSGLSSAVRDARTDGHHQTVKGLEPGRYFWQVR
                     QLDKSGAAIMKGPVVSFTVAERMGTPVVIAPAIREVVDMDASDALHFKWNRLRGANHY
                     RIELFQQDGDVLKPIVRHTTRDGEWKMTELEKLDVGNFCWTLQAFDRAPGATAVRKSP
                     LVKTCFEITLGKSLRKTKLKSPRVIYVK"
     gene            complement(2792..>3164)
                     /locus_tag="PKJ16_18440"
     CDS             complement(2792..>3164)
                     /locus_tag="PKJ16_18440"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HNV49026.1"
                     /translation="NVGQVLAGQIGSDERMEYTVIGDAVNLASRIEALNKPFGTDILI
                     TQDTYELVKDVFRVEPMQTIKVKGKVEPQQIYAVLGRTDDPTAPRSLDAVRALMGIEM
                     KGPIADHAVDEEEVKYEIIEK"
BASE COUNT          538 a          923 c         1007 g          696 t
ORIGIN      
        1 ccgccggcct tgaggatgaa attgaccggg atatcgaacc gcgttgcggc atagaggttc
       61 accccgaagg tcgatccctg aatgtcgaag gttgttcccg cagtgttttg ctttgccttg
      121 aattccatgg tgctcccctc gaactcaacg ccggtgtacc gaagcacgcc ccactcgccg
      181 aatgcgccgt gcgtccccac atcgtaggcg aggatgccgt tgaagtttcc gatggagtcc
      241 atgaaatagt tttgccagtt gccgctcaag ctgtaattca cgatgccgac gctcagcttg
      301 atgcccaggt taaaaaggtt cctactgaag cgcgtctcga tagcggtggt cccgcgcttg
      361 atttctatcg tttcccactc ggaccagctg ccgatcttcc tgaacttgtt gaccaccccg
      421 atgcggagac ggtacgttcc ggggggcaac ggtacggtga cggtgttggt ctccgtggat
      481 gtggcgatga tctccttgcc gtccttgtcg gtgatgcgaa tggcgtagcc gatggcgccg
      541 tctatcgcct cccagtcgat ccgcactccg tcggtggacg attgcccgga aacgggcggg
      601 gcgggcgcga caatcgccca tgccagcagg gcgatcgcga gaagcaggga gaatcgtgtc
      661 ttggtcggta tcatcggcct acttgacgta aatgacgcgc ggcgatttta acttggtctt
      721 cctgagggat ttccccaggg tgatttcaaa gcaggtcttt accagcgggc ttttccgtac
      781 ggcggtcgca ccgggcgcgc ggtcgaacgc ctgcagcgtc cagcagaaat ttcccacgtc
      841 gagtttctcg agttcggtca tcttccattc gccgtcccgg gtcgtatggc gcacaatcgg
      901 cttgagaacg tccccatcct gttggaacag ctcgatgcgg taatgattcg ccccgcgcag
      961 gcggttccat ttgaagtgca gcgcgtccga ggcgtccatg tcgacgacct cgcggatcgc
     1021 cggggcgatg acgaccggtg ttcccatcct ctcggcgacg gtaaatgaaa ccacgggccc
     1081 cttcatgatc gccgctcctg atttatcaag ctgacgcacc tgccagaagt atcgacccgg
     1141 ctccaggcct tttacggttt gatggtgccc gtcggtgcgg gcgtcacgaa ccgccgagga
     1201 gagtccgctg tctcgagaga cgacgaggtg gaactggcca ggcatttcgg tgcgcttcca
     1261 tgagaatgcg acggcggccg cagtgtcctc cccgtccggg gcgatggtcg cattgtcacg
     1321 cggcgcgatg atggcaatga cgtcggattc gatgatgcgg aactgacgcg cggacgaggt
     1381 gaccgactcg gaaccgccgc gaatcatgcc ggtcacccgc cagaagtagg gaccggtatc
     1441 gagcgcgccg gtgagcttgg cgaaattcgt agcgctcgtt cctgtgtgga tgatgttggt
     1501 aaagccgcga tcacgcgcga tatcgattcg cgacgatccg atctcgggga cggtccgcca
     1561 ggtgaaggtg atggttccgt ttttgagcga tatcttgttg aactgtctgg cgtcgggcgg
     1621 gaatacgagc tcgggcggat caagcgatgc ggtctgcgtg atggtgaagc gatgcacggg
     1681 gctggagacg ctctgtccgg cgccggaacg gcgcaccatg cagtagtagg ttccgctgcc
     1741 aagcgtgtcg agcgccgctc cggtggcgga cgtgtcgatc cgctggacga cgcggctcat
     1801 tgatgaatcg ctcgcgacgg taagcgtgta tgaggacgac aattcgctcc cggaccactt
     1861 gaagctcacg agcggctttt tggcattgta cgaataggtc gcgccgtcgt gcggggatat
     1921 cagcgagagg ggggcgtcgc gggtgatgga cagccgacgc acctcgctgt attcgatctt
     1981 gcgcgtcttc cggttcatgg cgcgcaggcg ccagtagtag atcccggagg gcagctcgag
     2041 cgtacgtccg tctcccgcga tccggcggat gctcacgatg tcgccgaatg atgggctctg
     2101 cgccacctcg tggtggatgt cggcggcgtc gcggacggcc tcccaggaga actggacccg
     2161 gtgccgcgcc gcggtcgtgg ttatgtgctc gttcggcgtc ggcgacagga gcttgaggtt
     2221 gagccggtag atcgtaaggg cgtccttcga aagaacggcc ttttcgtcct gtctgatgag
     2281 cttctccgtt tcaccgcggt tgatcttcgc cgtgccccgg ttgacggtca gattgagatc
     2341 ggtcccgccc atccgcgtaa ggctcacgtc gctcttgtcg atggaaacga ccgtatcctt
     2401 ggatttgatg ttcatcttct gcttggggtc ggcgtccccg cgcttggcgt tgatggagcc
     2461 ctgcgcgaac tcgatgtcga cctcgttttt cgaaaacgag atcaggatca tgctgttctc
     2521 gttgagcgcg atctcggtgc cgtccttgag cttgatcacc gcctcggaga gctcggccgt
     2581 ccggatggag tcgtgattgt ggacgggaac ggcctcggcg acgtcctccc agacgacctg
     2641 gctggcgtac ttgcgctgcg cctgctggcg cttgaaggtg atggtgccca ccacctcctc
     2701 cgaaccggcc ccgcgtcggc gcgtgaggtc gtagtagaag agacccgaga acaggacgat
     2761 gatgaccagg cccgccacga ggacggtttt gtcatttctc gataatttca tatttcactt
     2821 cttcttcatc gactgcgtgg tcggcgatcg gacccttcat ttcgatgccc atgagcgccc
     2881 gaacggcgtc aagcgagcgc ggcgcggtgg gatcatccgt tctcccgagc acggcgtata
     2941 tctgctgggg ttccaccttg cccttcacct tgatcgtctg catcggttcg acacgaaaca
     3001 cgtccttcac cagttcatag gtgtcctggg tgatgaggat gtcggtgcca aacggcttgt
     3061 tcagcgcctc gatccgggag gcgaggttga ccgcatcgcc gatcaccgtg tactccatgc
     3121 gctcatcgga gccgatctgg cccgccagga cctgcccgac attg
//
LOCUS       DAORHF010000366         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_35354_length_3135_cov_6.183766, whole genome shotgun sequence.
ACCESSION   DAORHF010000366 DAORHF010000000
VERSION     DAORHF010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35354_length_3135_cov_6.183766"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PKJ16_18445"
     CDS             complement(<1..555)
                     /locus_tag="PKJ16_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49027.1"
                     /translation="MKKLLIILMLAAVSLGTGCVYYVRGDLDYYNMAMDKNKEPKKIA
                     YRVNTNFYSKMYSMQQFFLASILQDESKPYNQYDPMNMYTGLKGDLPDVQVLSGNRTW
                     VMGQKEKNIINKVVGDLFSSNVLYTEKTLGKKPENLFFDVSVMFHEVSDKGDHLAGAM
                     ISGLTLWLFPNRFSRHNIYYAVDVY"
     gene            complement(588..1076)
                     /locus_tag="PKJ16_18450"
     CDS             complement(588..1076)
                     /locus_tag="PKJ16_18450"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HNV49028.1"
                     /translation="MERVPENILNIWQNIVTIVAETCGVDVCLITRFDPPEEECVNVS
                     GSAAGRFAPGERWSMSGSFAGTVFESKSRLLVGDAARDGRWERCSGVRGGMVSYLGYP
                     LFHGDGRVFGTICVLDSAVIRSGNRFDAIMKECKSMIEEHLRMMDSGEYAAVRGEVVR
                     NR"
     gene            1319..>3135
                     /locus_tag="PKJ16_18455"
     CDS             1319..>3135
                     /locus_tag="PKJ16_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV49029.1"
                     /translation="MNSGTVHAIQRGAPPETPAITIGEERFRTGLDRYHSASDPTGAL
                     FLAKMPVRENADTRAQGRYARELDASRALAGDGILAATESVENGRALLVASVDVIPLR
                     LAGRERVTGLANALAIGEQAAATLARIHKARMIHTGINPDSILIGSALPADGDGPIAV
                     LIGDLGLSRNADDVGSERRAPHPDLDPRYFSPESTGRINRVPDYTSDLYSLGVVLYEL
                     LAGVCPFASPRPADVIYGHIALIPTDPREHDASIPSSVAAILMKLLAKNSPDRYRTAE
                     GLRLDLARCRSELRATGTISHFSAGAADGAGILRRPSRLHGRDRERGELMERLARARD
                     GASCITLVTGQPGVGKTALTASLARDAGESGGMFVSGKFELTPGQAPHGALVQALSSL
                     VTRTLIKSDDVVGRWRDRLLDALQPNARIILDLIPELGHVLGEQPEVPITGPIETANR
                     LNTYLLRFVAACADDCRPLVLFLDDLQWADPANLAMIFSLATDPAMKHLHLIGAYRDI
                     EVDAAHPLSGTIDEIAAAGVSIHRLHLKPLAREHLRALAADVIDCGEETTDRLADILF
                     SRTRGNPLHSLILIRDLVDRGLLAFGTGGWRLDETELRSL"
BASE COUNT          602 a         1077 c          896 g          560 t
ORIGIN      
        1 gtacacatcc acagcgtaat aaatattatg ccgagaaaaa cgattgggga acaaccaaag
       61 cgtcaatccc gagatcatgg ccccggcaag atgatcaccc ttgtccgaca cctcgtggaa
      121 cataacggac acatcaaaaa aaagattctc cggctttttc ccgagcgtct tttcagtata
      181 gagaacatta ctgctaaaaa gatctcccac caccttgttg atgatgtttt tctccttttg
      241 tcccatgacc caggtgcgat tacctgacag tacctgcaca tccggcaaat cgcccttgag
      301 gccggtatac atattcatgg gatcatactg gttatatggt ttcgattcat cctgaaggat
      361 agaggctaaa aaaaactgct gcatcgaata cattttcgaa taaaagttgg tattcacccg
      421 gtacgcgatc ttcttgggct ccttgttctt gtccatggcc atgttatagt agtcaagatc
      481 gccccgtaca tagtagacac agcccgtccc aagggacacg gccgcgagca tcagaataat
      541 taaaagcttt ttcataggtc ttactcctcg tatttaatgt ataacgacta gcggttccgt
      601 acgacctcgc cccggaccgc cgcgtactcc cccgaatcca tcatccgcaa atgctcctcg
      661 atcatggact tgcactcctt catgatggcg tcgaagcggt tcccggagcg gatcacggcg
      721 ctgtcgagca cgcagatggt gccaaagacg cgcccgtcgc cgtgaaacag cgggtacccc
      781 aggtacgaaa ccatcccgcc gcgcacgccg ctgcagcgct cccatctgcc gtcgcgggcg
      841 gcgtccccca caaggaggcg cgacttcgat tcgaacacgg tccccgcgaa gctgccgctc
      901 atgctccagc gctctcccgg cgcgaagcgc ccggcggccg aaccgctcac gttgacgcac
      961 tcctcctccg gaggatcaaa cctcgtgatg aggcacacgt cgaccccgca tgtttcggcg
     1021 acgatagtga cgatattctg ccagatgttc agtatgtttt cgggtacgcg ttccatgccg
     1081 cgcgacgccc ccctcgtatc cgcccgcggg gacggacccc gcgcttcatc ggaaagatac
     1141 agaggatccc gccgtcgggg aagcggcatg taatgaacgc tatccggcat gtaatgaacg
     1201 ctacgtacga tgcaatgaaa ccggcatgcg gaaaaacgct tgcgctgaac gcgccatgcc
     1261 atgcacatga gaagcggcgt ttgacgccga tcgaccattc gggagccaca cccaggtcat
     1321 gaattccgga acggtacatg cgatacaacg gggcgccccg cccgagaccc cggccataac
     1381 gattggcgag gagcgcttca ggacgggact cgaccgctat cactcggcga gcgatccgac
     1441 gggcgccttg ttcctggcca agatgcccgt gcgggaaaac gccgacacgc gggcgcaggg
     1501 acggtatgct cgggagctcg atgcgtcgcg ggcgctcgcc ggggacggga ttctcgccgc
     1561 caccgagagc gtcgagaacg gtcgggcgct ccttgtcgcg tctgtcgacg tcatcccgct
     1621 ccggcttgcc gggcgcgaac gcgttaccgg cctggcgaac gcgcttgcga tcggggaaca
     1681 agccgccgcg acgctcgctc ggatccacaa ggcccgcatg atccataccg gcatcaaccc
     1741 ggacagcatt ctgatcggga gcgccctccc ggccgacggc gacggcccga tagcggtcct
     1801 gatcggcgat ctgggccttt cgcggaacgc ggacgacgtc ggatccgagc gccgggcgcc
     1861 gcacccggac ctcgatcccc gctacttctc gccggagagc accggcagga tcaaccgcgt
     1921 ccccgactat acgagcgacc tgtattccct cggcgtcgtc ctctacgaac tcctggccgg
     1981 cgtctgcccc ttcgcctcac cccgtcccgc ggacgtcatc tacggccaca tcgcgctgat
     2041 cccgaccgat ccgcgcgaac acgacgcatc gatcccgtcg tccgtggcgg cgatcctcat
     2101 gaagctgctg gcgaaaaatt cccccgaccg ctatcggacc gccgagggcc tgcggctcga
     2161 cctggcccgc tgccggagcg agcttcgcgc gaccggtacg atctcgcatt tctcggccgg
     2221 tgcagcggac ggcgcgggca tcctccgccg gccttcgagg ctacatggac gcgaccggga
     2281 gcggggcgaa cttatggaac gcctcgcgcg ggcccgcgac ggcgcatcgt gcatcacgct
     2341 cgtgacggga caacccggcg tcgggaagac cgccctgacc gcctcgctgg cgcgcgacgc
     2401 gggcgagtcc ggcggcatgt tcgtttcggg caagttcgaa ctgactcccg gccaggcgcc
     2461 ccacggggcc ctcgtccaag ccctgagctc gctggtcacc cgcacgctca tcaaatcgga
     2521 cgatgtcgtc gggcgctggc gcgatcggct cctggatgcc ctgcagccca acgcccgcat
     2581 catcctggac ctcatcccgg agctcgggca cgttctgggc gaacagccgg aagtgcccat
     2641 cacgggaccc attgagaccg caaaccgcct caacacctac ttgctgcgct tcgtcgcggc
     2701 ctgcgccgac gattgccggc ccctggtcct gttcctggac gacctccagt gggcggaccc
     2761 ggccaacctg gcgatgatct tctcgctggc gaccgatccc gcgatgaagc acctccacct
     2821 catcggcgcc tatcgcgaca tcgaggtgga cgccgcgcac cccctttcgg ggacgatcga
     2881 cgagatcgcg gccgccggcg tgtcgatcca ccggctccac ctgaagccgc tggcccgtga
     2941 gcacctgcgg gcgcttgccg ccgacgtgat cgactgcgga gaagaaacga ccgaccgtct
     3001 cgccgacatc cttttttccc ggacgcgcgg aaatccgctc cactcactga ttctcatccg
     3061 cgatctcgtc gacaggggac tgcttgcctt cggaaccggc ggctggcgcc tcgacgagac
     3121 ggagcttcgc tccct
//
LOCUS       DAORHF010000367         3118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_35680_length_3118_cov_3.959843, whole genome shotgun sequence.
ACCESSION   DAORHF010000367 DAORHF010000000
VERSION     DAORHF010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35680_length_3118_cov_3.959843"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKJ16_18460"
     CDS             <1..546
                     /locus_tag="PKJ16_18460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HNV49030.1"
                     /translation="LISSLVSLQASGAADDSTRAVLMDVESRIMSIARLYGQLYESGG
                     YARIDLRDYLTRIINGVQMGYMARQGVRIRASIDFATCSFKTASSLGIILNELLTNAF
                     KYAFPGERTGAVSVSLTRKDESFALHVEDDGAGLPPGFDLESSQGMGMQLVRLLAKQH
                     RGAAVVDPGGRASFTVTVVAD"
     gene            872..1333
                     /locus_tag="PKJ16_18465"
     CDS             872..1333
                     /locus_tag="PKJ16_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HNV49031.1"
                     /translation="MNCPKCDQPLEKKDLRGERVGHCASCNGWWLETADIACVSKAWK
                     EFPDLRKSNPRKRRETLAVCSLCRVELDGFYHDTGATTLLVERCPKCGGVWLDDGELK
                     RVTIYAKDARRGGEQPPVEKQMKDHLKKMGVWVIAAVVGIVLLVAVLVYLL"
     gene            1353..3068
                     /locus_tag="PKJ16_18470"
     CDS             1353..3068
                     /locus_tag="PKJ16_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49032.1"
                     /translation="MLTRRTCLALLAAIAIGQVFVSGSRADDALVTVKRLVSPRSASV
                     DRIIPSPDGARAAVLYAKGEARYIQVGDRLYAGDDSMSGFTFSANGARYAFWYRSPTS
                     TQTLYNACLSKPIATTHCCYDLATCNWSILGGGGKYTPIRKWSYYIQVDTETHGPFDN
                     LFITPRLHDPRGRHSDIRYASDSFVSFSPVSGDVAFGGMRDKQTFVRIANRSIGPYGY
                     SEMGHTNVYLHFSDDLGLFGYEYEKMERGDRFGVIRYAQIGGTTYGPYDFLKHLTVTA
                     NGAWAFAYQKGDGYYVKLKGADIGPFPVIRGFGLSDDGRRYYVWYREQQANYLRVNDA
                     VTRLSHDGDGITDIFANRGLSKIAVRYMKGSDYYIDIDGKTYGPSDYHGSIGIRFGDT
                     GDGYAVWHALPGRPARCYLHLPGKTIGPYASIKLERLSPDGLRYGIVYQKDGKRFAGT
                     TDATHGPFESVVETAFSDDARRCAVIYSSPKKIGSWDCYVRVDGKVFGPYRDARNFSF
                     SPDGRRYGLTFHAHGDGRMVSAVVDGTAIPRFAVAPAAFSFTKDNRAILAYCDNANVT
                     VREVR"
BASE COUNT          588 a          896 c         1003 g          631 t
ORIGIN      
        1 ctcatatcga gcctggtctc gttgcaggcg tcgggcgcgg cggacgattc gacccgcgcc
       61 gtgctcatgg atgtcgagag ccgcatcatg tccatcgcgc gactctacgg ccagctttac
      121 gagagcggcg ggtacgcccg gatcgacctg cgcgattatc tcacgcgcat catcaacggc
      181 gtgcagatgg gctacatggc ccggcagggc gtccggattc gcgccagcat cgatttcgcg
      241 acatgcagct tcaagaccgc ctcgtcgctg ggcatcatcc tcaacgagct tctcaccaat
      301 gccttcaagt acgccttccc cggagagcgc acgggcgcgg tgagcgtgag cctgactcgg
      361 aaggacgaat cgttcgcgct gcatgtcgag gacgacgggg cgggccttcc gcccgggttc
      421 gatctcgaat cgtcgcaggg gatggggatg cagctggtgc ggctcctcgc gaaacagcat
      481 cgcggcgcgg ccgtggtcga tcccgggggc cgggcgtcgt tcaccgtcac cgtcgtcgcc
      541 gattgagccg ttcgcggcga cttcgcgcgc accggtcgca catggtatcg gttggatcgg
      601 cgtatgatgg cgccatcgcg tttcggtaac gatgcccggt ggcgggaaaa actggttgcc
      661 aaatcaaaat ggtgggcgca tgagagcggt tgcagcacgc aagttcctgt catcgcgagg
      721 agcgcggcga cgaagcggcc cgatgtcttg aaccggaaga tgagcctgta gctgattgcg
      781 tgtgcggcag cgtttcatgg tgcggatcgc ttcgccgtgt tcgcggtgac attgaagtgt
      841 gaacagatcc gccaaggaaa cggtataatc catgaactgt cccaaatgcg atcaacccct
      901 cgaaaaaaaa gatttgcgcg gcgagcgggt gggccactgc gcctcgtgca atggctggtg
      961 gctcgagact gccgacatcg catgcgtctc gaaggcctgg aaggagtttc ccgatctccg
     1021 aaagagcaat ccccgcaagc gccgcgagac gctcgccgtc tgttccctct gccgcgtcga
     1081 gctcgacggc ttttaccatg atacgggcgc cacgacgctt ttggtggagc gctgcccgaa
     1141 gtgcggcggc gtgtggctcg acgacggcga gctgaagcgc gtgaccatct acgcgaagga
     1201 cgcccgacgc ggcggcgagc agccgccggt tgaaaaacag atgaaagacc atctgaagaa
     1261 gatgggcgtc tgggtgattg ctgcggtcgt cggcattgtc ctgctggtgg ccgtgctcgt
     1321 ctatctgctg taagcgcgcg ggggaggggc cgatgctgac gcgacgaaca tgtctcgccc
     1381 tcctggccgc gatcgccatc ggtcaggtgt tcgtttcggg ctctcgggcg gatgatgcgc
     1441 tcgtgaccgt caagcgtctc gtctccccgc gctccgcttc cgtcgaccgc atcataccct
     1501 cgcccgacgg cgctcgcgcc gcggtcctgt atgcaaaggg cgaagcgcgg tatatccagg
     1561 tgggcgaccg gctctatgcg ggcgatgata gcatgtccgg tttcaccttc agcgcgaacg
     1621 gcgcgcgata cgccttctgg tatcgcagcc ccacctcgac gcagacgctg tacaatgcgt
     1681 gcttgtcgaa gcccatcgcc acaacccatt gctgttacga ccttgcgaca tgcaattgga
     1741 gcatattggg agggggcggg aaatacactc cgattcggaa gtggtcgtat tatattcagg
     1801 tcgatacgga aacgcatgga ccatttgaca atctcttcat cacgccgcgg ctgcacgatc
     1861 cgcggggacg ccactctgat attcgttacg caagcgattc cttcgtgtcg ttcagtcccg
     1921 tgagtgggga tgtcgctttc gggggcatgc gggacaagca aacgtttgtc cgcatagcca
     1981 acagatcgat cggtccctat gggtacagcg agatggggca cacgaacgtc tatctgcatt
     2041 tcagcgacga tctcggtctc tttggctatg agtatgaaaa gatggagcgc ggggatcggt
     2101 ttggcgttat caggtatgcg caaatcggcg gaacaaccta cggcccgtat gatttcctaa
     2161 agcatctcac cgtaaccgcg aacggggcat gggccttcgc ctaccagaag ggtgacggat
     2221 attatgtgaa gctcaagggc gctgatatcg gcccattccc cgttatcagg ggcttcggcc
     2281 tgagcgatga tggcaggagg tactatgtct ggtatcgcga gcagcaggcg aactatcttc
     2341 gtgttaacga tgcagtgacc cggctgtcgc acgatggtga tgggataact gatatatttg
     2401 cgaaccgggg cctttcgaag atcgcggtga gatacatgaa agggagcgat tactatatcg
     2461 acatcgacgg gaagacctac gggccctcgg attatcacgg gagcatcggc atccgtttcg
     2521 gcgatacggg cgacgggtac gccgtctggc atgcgctgcc cgggaggccc gcacggtgct
     2581 atctgcacct gcccggaaag accatagggc cctatgcttc gatcaagctc gagcgcctgt
     2641 ccccggacgg cctgcgctac gggattgtat atcaaaagga cggaaagcgc tttgccggga
     2701 cgacggatgc gacccatggt ccatttgaaa gcgtcgtcga gacggcattc agcgacgacg
     2761 cgcgtcgatg cgccgtcatc tacagttcgc ccaaaaaaat cggcagctgg gactgttacg
     2821 ttcgcgtgga cgggaaggtg ttcggcccgt atcgggatgc gcgaaatttt tcgttctcac
     2881 cggatggccg tcggtatggt ctcacctttc atgcgcacgg tgacggccgg atggtgtcgg
     2941 cggtcgtcga cggtactgcc attccgcgct tcgcggtcgc gccggcggcg ttttcgttca
     3001 cgaaggacaa tcgcgccatt ctcgcctatt gcgacaatgc gaacgtaacg gtgcgcgagg
     3061 tccggtaacg gacgggggat gattccgtcg cggccgcgcc ggtttcctcc tcaggatt
//
LOCUS       DAORHF010000368         3081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_36377_length_3081_cov_4.271646, whole genome shotgun sequence.
ACCESSION   DAORHF010000368 DAORHF010000000
VERSION     DAORHF010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36377_length_3081_cov_4.271646"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1265
                     /gene="argJ"
                     /locus_tag="PKJ16_18475"
     CDS             75..1265
                     /gene="argJ"
                     /locus_tag="PKJ16_18475"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HNV49033.1"
                     /translation="MPSDSPALASVPGFTFSAIKCGIRYPDRLDLALIYSQTPCVACG
                     TFTANRLAAAPVRLCRERIDRPIHAILINSTNANACTGDTGYENAITLASAVSRRLSI
                     SDDSVLMASTGIIGVQLPVDTIERGIPKLIDGLSTGQGPLIKQAIMTTDTFPKEAGAY
                     FTHDGRRYAVAGVAKGAGMIAPNMATMLAFVITDAPIARKDLDPIFRRCIDTTFNAIT
                     IDGDMSTNDTALILAPDTGQPLTDETALESFEAALLHTTRTLAEMIVRDGEGATRLVT
                     VTVRGAKTDDDARTAARAIAESLLVKTALFGRDPNWGRIACAAGYSGAAFDERTLSIS
                     IESVQLLTDGVPLSFDKTALLEIMSRREYEIHVNLGLGSGRFSFMTTDLSYDYVKINA
                     EYST"
     gene            1318..2409
                     /locus_tag="PKJ16_18480"
     CDS             1318..2409
                     /locus_tag="PKJ16_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49034.1"
                     /translation="MKRIFVHILLLIVTCAIATSVEARDREKVVLYYFKDLSASTDYS
                     YYSYIIPDSIAVDLKRSKRFDVTTFPVSADTLPKGATNDIRNSHIRMLSEKGREFSAA
                     YIICGSYHIKNRRIFIDTQIFIVERQEIVEVSESSSELGTMLFAMIDSITKKINSELG
                     RYAPHKTVDAPVMTSSPFYNFIRGTTFGMSYGTVKFQEEWGRYFDTASMVACHLSYDL
                     NNIGLFRDIPVLSDLSLGAEYSFFNTKPISEYEHYLNMWGALISVSYQIRFTDLFIAG
                     IWAGWGEAHTTYTIFPYSIDSGSNDNGPFVAPSQKKSSTDNYIGGGLSLNVILDPLVI
                     TSGISLRKIYNSPRPITFSVVSFGLGYRI"
     gene            2499..>3081
                     /locus_tag="PKJ16_18485"
     CDS             2499..>3081
                     /locus_tag="PKJ16_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV49035.1"
                     /translation="MNSKKLQSIINDDGAYLFQTYTRQPVSFARGEGSYLYDQDGRRY
                     IDFLSGIAVNLLGYGHPRLLKTLHKQLDVMLHTSNLFLNSEQIAAAKLLSKTAFPGRT
                     FFSNSGTEANEAAIKLARRHGRTVSNEKFRIISFEGSFHGRTFGAMAATGNEKIKKGF
                     EPLMEGFTILPYNDIDSFLSVADGAKYCAVLTEL"
BASE COUNT          674 a          925 c          771 g          711 t
ORIGIN      
        1 gacgggtatt gtttactatt ttattttatt taattttttg tcaaagactt tttatgcttc
       61 ccgtggagga ttcaatgcca tcggattcac ccgccctggc aagcgttccg ggcttcacct
      121 tttctgcgat caaatgcggt atccgatatc cggaccgtct cgatctcgcg cttatctatt
      181 cacagacacc ctgtgtcgcc tgcggcacct tcaccgccaa tcgccttgcc gccgcgccgg
      241 tccggctctg ccgcgagcgg atcgacaggc cgattcacgc gatccttatc aactcaacga
      301 acgcaaacgc atgtacgggc gataccggtt atgaaaacgc gatcacactt gcgagcgccg
      361 tgtcccgtcg tctttcgatt tcggacgatt cggtgctcat ggcctccacc gggatcatcg
      421 gcgtccagct gccagttgac acgatcgaac gtggaatccc gaagctcatc gacgggcttt
      481 ccacgggcca ggggccgctc atcaagcagg ccatcatgac gacggacacc ttcccgaagg
      541 aggccggtgc ctacttcacg cacgacggtc gccgttatgc cgtcgccggc gtcgcgaagg
      601 gcgcgggtat gatcgccccg aacatggcca cgatgctcgc cttcgtcatc accgatgcgc
      661 ccattgcgcg caaggatctc gacccaatct ttcgtcgctg cattgatacg accttcaatg
      721 cgataacgat cgacggagac atgtcaacca acgacaccgc gctcatcctc gcccctgaca
      781 ccggccaacc gctcacggac gaaacggcgt tggaatcgtt cgaagcggcc cttctgcata
      841 cgacacgcac ccttgcggag atgatcgttc gcgacggcga gggtgcgacc cgactggtga
      901 ccgtgaccgt ccgcggcgcg aagaccgacg acgatgcccg caccgccgcc cgcgcgatcg
      961 ccgaatcgct tcttgtgaaa acggccctct tcggacgcga cccgaattgg ggtcgcatcg
     1021 cctgcgccgc cggctactcg ggagccgcgt tcgacgagcg cacgctttcg atatcgattg
     1081 agtcggttca actccttacc gacggcgttc cgctttcgtt cgataaaacc gcactcctag
     1141 aaataatgtc gcgacgggaa tatgaaatcc atgttaacct gggtctggga tcgggccgtt
     1201 tttcattcat gactaccgat ctttcgtacg attatgtgaa gataaacgcc gaatactcga
     1261 catgacggga tcgacattac tcggattgca ggcacattcg cctactgatg cgcgcacatg
     1321 aaacggattt tcgtacatat attgttgctt atcgtcacct gcgcgatcgc cacctccgtc
     1381 gaggcgcgcg atcgcgagaa ggtggtcctg tactatttca aggatctcag cgccagcaca
     1441 gactacagtt attattcgta catcatcccc gattcgatcg ccgtcgacct caagcgctcc
     1501 aaacgctttg acgtcaccac cttcccggtg agcgccgata cgctgcccaa gggcgcaaca
     1561 aacgacattc gaaacagtca catacggatg ctttcggaaa aaggtaggga gttttccgcc
     1621 gcttacatta tttgcggatc ataccacatc aagaaccgtc gaatattcat cgatacgcaa
     1681 attttcatcg tcgagcgcca ggagatcgtc gaggtctcgg aatcgagctc ggagctgggc
     1741 accatgcttt tcgccatgat cgattccatc acgaaaaaga tcaacagcga gctgggccga
     1801 tatgctcccc acaagacggt cgatgcgccg gtcatgacgt catcgccgtt ctataatttc
     1861 atccggggca cgacctttgg catgagctat ggcactgtca aattccagga ggaatggggc
     1921 cggtatttcg atacggcctc gatggtcgcg tgccatcttt cgtatgacct caacaacatc
     1981 gggctctttc gcgacatccc ggttctgtcc gacctctcgc tgggcgcgga gtactcattc
     2041 ttcaatacga aaccgatcag cgaatacgag cactatctga acatgtgggg cgcactgatc
     2101 tcagtttcct accagatccg cttcaccgat cttttcatcg ccggcatttg ggccggatgg
     2161 ggcgaagcgc acacgacata taccatattt ccgtattcga tcgactccgg ctcaaacgat
     2221 aacggcccat ttgttgctcc atcgcagaaa aaatcctcga ccgacaatta catcggcggc
     2281 ggtctctcgt tgaacgtgat tctcgatccg cttgtcatca cctcgggcat ttcgctcagg
     2341 aagatttata attccccgcg cccaatcacc ttctcggttg tttccttcgg tttagggtat
     2401 cgcatctaag ccgagtgaat ttcacctcgt cgggcaatta atgtattgac aagccattgc
     2461 cgcgttcatg tggtaatatt ttttcaggcg gggacgatat gaactcaaaa aaacttcagt
     2521 cgattattaa tgacgacggc gcatatcttt ttcaaacgta cacgcgtcag ccggttagct
     2581 tcgcacgagg cgagggatca tacctgtacg atcaggacgg ccgtcgatat atcgattttc
     2641 tctccggcat cgccgtcaat ctgcttggct acgggcatcc gcggctcctg aagacgcttc
     2701 acaagcagct cgatgtcatg ctgcacacct cgaacctgtt tctgaactcg gaacaaatcg
     2761 ccgcggcgaa actgctttca aagaccgcct tcccgggccg caccttcttt tcgaacagcg
     2821 gcaccgaagc gaacgaagcg gccatcaagc ttgctcgtcg ccatggccgc accgtatcga
     2881 acgagaagtt ccgcattata tcattcgagg gctcgttcca tggccggacc ttcggcgcga
     2941 tggcagcgac cggaaacgag aagatcaaga agggtttcga accgctcatg gaaggattca
     3001 ccatccttcc atataatgac attgactcgt ttctctcggt cgcagacggg gcgaagtatt
     3061 gcgctgttct aaccgagctc a
//
LOCUS       DAORHF010000369         3061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_36754_length_3061_cov_5.933134, whole genome shotgun sequence.
ACCESSION   DAORHF010000369 DAORHF010000000
VERSION     DAORHF010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36754_length_3061_cov_5.933134"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="PKJ16_18490"
     CDS             complement(<1..462)
                     /locus_tag="PKJ16_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49036.1"
                     /translation="MTSPQRRVALWIIVLCAVLAAHCAKSDQAEKQAPAGAEMEDATL
                     ATKAEKSKAAAPAAPGKVRQSSDESAANRRDDADRGLQSFMIAPMDLAKERLLEYRIT
                     LTYECKDLNRARQELLGIIAKYGYIRSSSATTETRNPSLDADLSVKEALLYD"
     gene            648..1976
                     /locus_tag="PKJ16_18495"
     CDS             648..1976
                     /locus_tag="PKJ16_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HNV49037.1"
                     /translation="MAGIRTQAATRELFGWAMFDFANSSYTTVIITVVYSVIFPRIIV
                     GDAPTFPYGNFLWSVALAVSNCVVLLCAPVLGAVMDYSAAKKRFLLVSTAITVLATAA
                     LFCVRPGDVWLGIALIVCSNIGFMLGESFIASFLPALGPPERLGAISGYAWGMGYFGG
                     LASTAIVLFGLGALEAENFSNLRYVGPITAVFFAIAAIPTFIWLREPGVPRTLPGIAA
                     YARVGFQRLRATLGALGDFRDLAVLLLSYLFAYAGLSVVISFAFIYGDQVVRWSPMVQ
                     MSMFIVTQVTAACGALAFGHIQDRAGPKPAYAATLLLWITAVVLIFFVDDITAAANRL
                     GGEWQVQHLFLVIGSVAGLGLGATQSSCRAMVGVLSPLSKSGEFFGLWGMANRLAAVC
                     GLMALGFLQAAFGLRNAFLICIAFFAAALAIGLMVNVDRGRAAARSHEGE"
     gene            1987..2679
                     /locus_tag="PKJ16_18500"
     CDS             1987..2679
                     /locus_tag="PKJ16_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005329902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HNV49038.1"
                     /translation="MDSFLVIGHRGACGYEPENTLRSFEKAIALGAHMVELDVHRCRT
                     GELVVIHDNRLERTTNGSGYVAEKTLDELRALDAGGGERIPLLREVVERVGVRAAINI
                     ELKGAGTAGPVSELIERTVGSGGYDTSHFLVSSFDHRELARFAEINRRVRIGALVEGV
                     PLGLAAFAQELGAFAVNAGLDFVTDEFVADAHRRGMRFFVYTVNHPEDIDRMRRMGVD
                     GIFTNYPDRASP"
     gene            complement(2813..>3061)
                     /locus_tag="PKJ16_18505"
     CDS             complement(2813..>3061)
                     /locus_tag="PKJ16_18505"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49039.1"
                     /translation="PAKNGQWTEYFPDGTTKKIEANYMLNKLNGEYKEYYPTGKIKAE
                     GEYMTNKKNGEWKFYLQDGSVDEENSGRYMNDRKWKMK"
BASE COUNT          446 a          915 c         1013 g          687 t
ORIGIN      
        1 gtcgtagagc agggcctcct tgacggagag gtccgcgtcc agcgacgggt tgcgcgtctc
       61 ggtggtggcc gagctcgaac ggatgtagcc gtatttcgcg atgatcccga gaagctcctg
      121 acgcgcgcga ttgaggtctt tgcactcgta ggtgagggtg atgcggtatt ccagcaagcg
      181 ctccttcgcc aggtccatgg gggcgatcat gaaggactgc agcccgcgat cggcatcgtc
      241 gcgcctgttc gcggcgcttt catccgagct ttgccgaacc ttccccggcg cggcaggagc
      301 cgcggccttg ctcttctcgg ccttcgtcgc aagcgttgcg tcttccattt ccgcaccggc
      361 gggcgcctgc ttctccgcct gatcgctttt cgcgcagtgc gctgcgagaa cagcgcagag
      421 aacgatgatc cagagtgcga ctcgtcgttg tggtgatgtc acgatcggtt cctccctgtg
      481 tggtgtccgt gcgcggcgac cgatggcgcc gccgtcgcgc gcctgtccat gagaccgcgc
      541 ccccgccgcc gcggttccca ggatcgctga aaaatatggg cgtgtcaacc gtaatccggc
      601 caatcttctg gttgacaaaa tcgcatctgc gggggccaca tcgtgcgatg gcggggatac
      661 ggacacaggc ggcaacgcgc gagctcttcg ggtgggcgat gttcgatttc gcgaactcgt
      721 cctataccac cgtcatcatc accgtggtgt acagcgttat cttcccgcgg atcatcgtcg
      781 gcgacgcccc cacgttcccg tatggcaact tcctctggag cgtcgcgctt gccgtgagca
      841 attgcgtcgt gttgctgtgc gcgcccgtgc tgggggcggt catggactac tccgcggcca
      901 agaaacgatt tctgctcgtg agtacggcga tcacggtgct cgcgacagcc gccttgttct
      961 gcgtgcgtcc cggcgatgtg tggctgggca tcgcgctcat cgtctgttcg aatatcgggt
     1021 tcatgctggg agagtcgttc atcgccagct ttcttcccgc gctggggccg ccggagcggc
     1081 tcggggcgat atccggctac gcttggggga tgggctactt tggcggtctt gcctcgacgg
     1141 caatcgttct cttcgggctc ggcgcgctcg aagcggaaaa cttttctaat ctgcgatacg
     1201 tgggacccat tacggcggtg ttttttgcca tcgccgccat tcccacattt atatggcttc
     1261 gcgagccagg cgttccgcgg accctgcccg ggattgccgc atatgcccgt gtcggattcc
     1321 agcgactcag ggcgacgctt ggcgcgctgg gcgattttcg cgatctcgcg gtgctgctcc
     1381 tgtcctacct gttcgcttat gccgggctct cggtggtcat cagcttcgcg ttcatctacg
     1441 gcgaccaggt ggtgcgctgg agcccgatgg tccagatgtc gatgttcatc gtcacccagg
     1501 tgacggcggc ctgcggcgcg cttgcattcg gccatatcca ggaccgggcg ggccccaagc
     1561 ccgcctatgc ggcgacattg ctgttgtgga tcacggccgt cgtgctcatt tttttcgtgg
     1621 acgatatcac cgcagccgca aaccggctcg gcggcgaatg gcaggtgcag cacctctttc
     1681 tcgtcatcgg ttcggtggcc ggcctggggc tcggggccac gcaatcgtcg tgccgggcga
     1741 tggtgggcgt tctctcgccg ctgtcgaaat cgggggagtt tttcgggttg tgggggatgg
     1801 ccaatcgact tgccgccgtg tgcgggctca tggcgctggg ttttctccag gccgccttcg
     1861 ggctcaggaa tgcgtttctc atctgcatcg cgttcttcgc cgcggcgctt gcgatcgggc
     1921 tcatggtgaa cgtggatcgc gggcgtgcgg cggcgcgatc gcatgaaggg gagtgagatg
     1981 ccgaatatgg attcgtttct tgtcattgga catcggggcg catgcggata cgagccggag
     2041 aacacgctcc gctcgttcga aaaggcgatt gcgctgggcg cgcacatggt ggagctcgat
     2101 gtgcaccgct gccgcacggg cgaactcgtg gtgatccacg acaaccgact ggaacgcacg
     2161 acaaacggga gcggatacgt ggccgagaaa accctcgacg agctcagggc gctcgatgcc
     2221 gggggcggcg agcggatccc ccttcttcgg gaggtcgtcg agcgtgtcgg cgtgcgcgcc
     2281 gcgatcaaca tcgagctcaa gggagcggga acggccgggc cggtgagcga gctcatcgag
     2341 cggacggtcg ggtccggcgg gtacgacacg agccactttt tggtttcgtc gttcgatcac
     2401 cgagagcttg cgcgctttgc cgagataaac cgccgggtgc gtatcggcgc gctggtggag
     2461 ggcgtgccgc tgggccttgc ggcctttgcg caggagctcg gcgccttcgc agtcaatgcc
     2521 gggctcgatt tcgtcaccga tgagttcgtt gcggacgccc accgccgggg catgcgcttc
     2581 ttcgtataca cggtgaacca cccggaggac atcgaccgga tgcggcgcat gggcgtcgat
     2641 gggatcttta cgaattatcc cgaccgggcg tcgccgtgag gtcgcgcgaa gcggatcata
     2701 ctggaacagc ggcgtagtgc cgacgcgggt ataatgcgaa aggcgaggtt gatgtcctcg
     2761 cctttcgtgt tcggtgcggc tgaatgcggc cgcgcgtatg tgtcgttgcg cgctacttca
     2821 tcttccactt gcggtcgttc atgtatcgcc cggagttttc ctcgtcaacc gagccgtcct
     2881 ggagatagaa cttccactcg ccgtttttct tgttggtcat gtactcgccc tcggccttga
     2941 tcttgccggt gggataatac tccttgtact cgccgttgag cttgttaagc atgtaattcg
     3001 cttcgatctt cttggtggtg ccgtccggaa agtattccgt ccactggccg ttcttcgccg
     3061 g
//
LOCUS       DAORHF010000370         3051 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_36964_length_3051_cov_3.653538, whole genome shotgun sequence.
ACCESSION   DAORHF010000370 DAORHF010000000
VERSION     DAORHF010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36964_length_3051_cov_3.653538"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKJ16_18510"
     CDS             complement(<1..552)
                     /locus_tag="PKJ16_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49040.1"
                     /translation="MRRIILVVVALSMVLFSHRVDAHVTDTARTWNFLEFRAKFAPDL
                     GVRIRPEHIYEHYRRDGEPLQTTEYYVYAGIFYDLPVSKYFMLNYALDYCYKGMPVHS
                     KYRDTSWQSNYPYEHAVYVWVTPTVLLGDFIIQTRLALYNQLFIGNTERWRGDAKLQG
                     YDASEASGYAATFRGMLQLSYRVT"
     gene            complement(558..1301)
                     /locus_tag="PKJ16_18515"
     CDS             complement(558..1301)
                     /locus_tag="PKJ16_18515"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HNV49041.1"
                     /translation="MIGNSKKLFARIVPIIITALAVGCTDVKDSNTPAGPPLEGLIAY
                     YPFTGNSEDESGNGRHASIRGNPRLTADVNGYLDNAYAFNGSGDFMDTPIYHLPTEFT
                     LAAWIKVNDTSSENPIISKNSSDGAPENGCEFYMSVLPLLHIRFVVSDGTVFSVVSTQ
                     RIKVGEWAHVAVRFRENGRSVIIDGQYDTLVTGFVSRAQKPMQIQIGRYHPDTGLLHF
                     NGSIDEVLIYGRALSDGEIRAIYDFGRPW"
     gene            1493..>3051
                     /locus_tag="PKJ16_18520"
     CDS             1493..>3051
                     /locus_tag="PKJ16_18520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HNV49042.1"
                     /translation="MEPYLRVLHLDAASGFYRIEKFPVGAFFGPVDLGLHLSKRFNSL
                     NIGTGLFAGSIFPGSNRMVFTGFSPCWGSFYISSMGGAGLVFDDLGINLLAIRNRAPV
                     PSLLCLNRVHGEEVQIDIAPVDCTAVWGAEPGGVYSMMRHSLDRFGDRYADSPRVLAV
                     GPAALATDMGAIASAPIKDGEISHIDTWAGRGGFGSKLLQEHGIAAIVYGGTVIDEDF
                     RDRKVADEWFQNKFQKKLAAKDMEATTKYRFDPKFDTGGTFGVNYAGMGGRIIAFNYR
                     SIYMTEDERRSLHSSFILDHYLKQFNDETIKTKSQKTCGEPCAAVCKKYRDEYKKDYE
                     PYQTMGPLCGVFDQRAAERLVRHADMLGFDSISIGGTLAWLMECLDAGHIAPAEIGVS
                     GTPRFSPTGFSVVVDSAHNAELGIAILDSIVGHRGLLERTEGMRKLARGLARDRGRAL
                     LDPFIYTANARTGWMVPNQYWTPGVLAPMPIMGKYYMHYGAEFHPPRELGRQCAKRLI
                     GELVMDNAGFCR"
BASE COUNT          655 a          900 c          848 g          648 t
ORIGIN      
        1 tgtaacccgg taggagagtt gcagcatgcc gcgaaacgta gcggcatatc ccgatgcctc
       61 gctcgcgtcg tatccctgca gtttggcgtc gccccgccag cgctcggtgt tgccgataaa
      121 gagttgattg tacaacgcca accgcgtctg aatgatgaaa tcccccagca gcaccgtggg
      181 cgtgacccac acgtagaccg cgtgttcgta gggatagttc gactgccagc tcgtgtcgcg
      241 gtatttgctg tgtaccggca ttcccttgta acagtagtcg agcgcgtaat tgagcatgaa
      301 atatttgctc accgggaggt cgtagaaaat ccccgcgtac acgtaatact ccgtcgtctg
      361 tagcggctcg ccgtcgcggc gataatgctc atagatatgc tcggggcgaa tacgcactcc
      421 gagatcgggg gcgaatttcg cgcgaaattc gagaaagttc cacgtgcgcg cggtatcggt
      481 cacgtgcgcg tcgacgcgat gcgagaacag caccatcgac aatgccacaa cgaccagtat
      541 gatccttcgc atgccgatta ccatggtctc ccgaaatcgt atatcgccct gatctcgcca
      601 tcgctcaggg cgcgtccgta aatcagcacc tcgtcgattg acccgttgaa atgtagcagg
      661 cctgtatcag ggtgatatcg cccgatctgg atctgcatgg gcttttgcgc acgactcacg
      721 aagccggtca cgagcgtatc atattgaccg tcaatgatga ccgatcgtcc gttctcccta
      781 aaacgaacag caacatgcgc ccactcgcca actttgattc tttgcgtcga cactaccgaa
      841 aacaccgttc catcgctcac cacaaatcta atatgcaaaa gtggaagcac gctcatataa
      901 aattcacacc cattctccgg tgccccatcg cttgagttct ttgagataat tgggttctcg
      961 ctcgacgtat cgttcacttt gatccatgcc gcaagcgtaa actcggtcgg gagatgatag
     1021 atcggcgtat ccatgaaatc gccgctccca ttgaaggcat aggcgttgtc gagatagccg
     1081 ttcacatcgg ccgtcaagcg cgggtttccg cggatcgacg catgcctgcc attgccgctc
     1141 tcatcttcgc tattgccggt gaatggatag tacgcgatca atccttcaag aggcgggccg
     1201 gccggtgtat tgctgtcctt gacgtccgtg caaccgacag cgagcgctgt tatgataatg
     1261 ggaacaatac gagcgaaaag cttcttgcta ttaccaatca tatcgttacc gtaattattc
     1321 aaattgcaaa acacggagca taatgaagaa tatatagtac ttttcggaaa aaacaagaat
     1381 tttatccatc aattcacatg ttcagatgta cgaaacgact gagtaattat tctgcttgac
     1441 acatcggttt caacgcatag agcttaggag agactgttcg gaggaagccg ccatggaacc
     1501 ctatctccgc gttttgcacc ttgacgcagc gtccggcttt tatcgaatcg agaaattccc
     1561 cgttggcgcg tttttcggtc cggtggacct tgggttgcat ctgtcaaaac ggttcaacag
     1621 cctcaatatc gggacaggcc ttttcgccgg atccatcttt cccggatcaa accgcatggt
     1681 cttcacggga ttctcgccct gctggggaag cttttatatc tcgtcgatgg gtggcgcggg
     1741 cctcgtcttc gacgacctcg gcatcaacct gcttgcgata cgcaatcgcg cgccggtgcc
     1801 ctccctcctc tgcctcaatc gcgttcacgg cgaagaggtg cagatcgaca tcgcgccggt
     1861 cgactgcacg gcggtatggg gcgcggagcc cggcggcgtc tattcgatga tgcgccactc
     1921 actcgatcgc tttggcgatc gctacgccga ttccccacgc gtactcgcgg tgggtcctgc
     1981 ggctctggcc accgacatgg gcgccatcgc gtcggcgccg atcaaggacg gcgagatttc
     2041 gcacatcgac acctgggccg gacgcggcgg tttcggcagc aagctgctcc aggagcacgg
     2101 catcgccgcc atcgtgtacg gcggaacggt catcgacgag gacttccgcg accggaaggt
     2161 cgccgacgaa tggtttcaga acaaattcca gaaaaagctg gccgccaagg acatggaggc
     2221 taccaccaag taccgcttcg atccgaagtt cgacaccggg ggcaccttcg gggtgaatta
     2281 tgcgggcatg ggcggccgca tcatcgcctt caactatcga agcatctaca tgaccgagga
     2341 cgaacggcgc tcgcttcatt catcgttcat tctcgaccac tacctcaagc agttcaacga
     2401 cgaaaccatc aagaccaaga gccagaagac ctgcggcgag ccgtgcgcgg ccgtgtgcaa
     2461 aaaataccga gacgagtaca agaaggacta cgagccctac cagacgatgg ggccgctgtg
     2521 cggcgtgttc gaccagcgtg cggccgagcg gctggtccgc catgccgata tgctcggttt
     2581 cgacagcatt tcaatcggcg gaacgctcgc atggctcatg gaatgcctcg atgcgggcca
     2641 tatcgcaccg gcggagatcg gcgtgtccgg aacgccgcgt ttctcgccga ccggcttcag
     2701 cgtggtggtc gattcggcgc ataatgccga gctcgggatc gccatactcg actcgatcgt
     2761 cggacatcgc ggtctcttgg agcgaaccga ggggatgcgc aagctcgccc gcggcctggc
     2821 gcgcgatcgc gggcgcgccc tgctcgaccc gttcatttat accgcgaacg cacggacggg
     2881 ctggatggtc cccaaccagt attggacgcc cggcgtgctc gcgccgatgc ccatcatggg
     2941 aaaatattac atgcactatg gcgcggaatt ccatccgccg cgcgagctgg ggcgccagtg
     3001 cgcgaagcgg ctcatcggcg agctggtcat ggacaatgcc ggcttctgcc g
//
LOCUS       DAORHF010000371         3026 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_37473_length_3026_cov_4.060922, whole genome shotgun sequence.
ACCESSION   DAORHF010000371 DAORHF010000000
VERSION     DAORHF010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37473_length_3026_cov_4.060922"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PKJ16_18525"
     CDS             <1..589
                     /locus_tag="PKJ16_18525"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HNV49043.1"
                     /translation="GFVAFSSSHVFGSPSQSAHRDDDEPHPISTFGDSLALGESHIRA
                     ASANSLIVRIAELCHPDAWFIGGAVRSLCAGKRVTVVRDWHVSPTSLSDCANALVGLL
                     LKDHHGIVNVSCAGSDTMRSVIERAAGRVSDLRGVDATSMIDEIDAVEFVTPYDFPLS
                     AVLDISLLSRVASCNPRPWQEALRECVEAIIASGQ"
     gene            complement(578..2059)
                     /locus_tag="PKJ16_18530"
     CDS             complement(578..2059)
                     /locus_tag="PKJ16_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49044.1"
                     /translation="MQITCSTCDSQYTLTDAQVSGLRHSIFQCRSCGKYIKIAFCQHC
                     NCLYSITFTTASSPQYRLTCRRCGKQFVVNFPTVAPVAVDATTQRAQAPATRPKKNDG
                     TIAQKIGRLVTVVSIFRKKPADPSEKTAKRRVDTPVSRNTEPVFSVDTFRDFTLREIF
                     TVCASSFTLPKIGAGVIGTVLLALLLIVYARIDTLVVMHVVPKTHASIRGVLQLVPIL
                     IVFVAYVTIAAVISRITIKDLFRQNYADAGSLPRFIIRALPSICITNVLVLFSAGALF
                     IVFGHIPLIGPFFYSLIFLPVYLLSLGIIIVAIIGFWFYPPLVAYRKSGIAENAINLF
                     HFIRRHNFNLLFYIPILSFTGLLVVGVLYYVHVGAFSLTAFLTESIIPHEGAAVFSAI
                     PSDLLRVFDISITGRGQGLLRSFHSNAGAVHQIAGLFVGIGLFSLSLVLYAMFMSITA
                     TLSTHVFIMLERGRDFEDWKKAVVLITLVLLLAGCYLVKRLLS"
     gene            complement(2062..>3026)
                     /locus_tag="PKJ16_18535"
     CDS             complement(2062..>3026)
                     /locus_tag="PKJ16_18535"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HNV49045.1"
                     /translation="FLDSPTAFEGVRKSWTPTNYNKSYSGKVLVRKALSSSLNVVSVL
                     AYDEIGGDRIAKFSGHLMGLPDNRFDVNPTLALGTTEVSPYEIALGFAVFANGGKSVR
                     PYCIRYILDRHGKRINDTEAEIEKIHKKQLVHPGVSYILTDLLRSVVDSGTGHGPIRR
                     AAGFRLPAAGKTGTNSDFKDAWFVGYTADMVTAVWVGCDSPKFTLGHGQSGAVAAAPI
                     WGSYMKSIYATRKPAPFPRRPKGITYCTVCTKTGMLPVPGCASRTEIFLEGTQPTDSC
                     SLEHDDPDSVFELARKRKARMIERETGKNSREDIESLNLEMLDD"
BASE COUNT          711 a          799 c          855 g          661 t
ORIGIN      
        1 cggcttcgtc gcgttcagct cctcgcatgt cttcggttcc ccgtcgcagt cggcgcatcg
       61 tgacgatgac gaaccgcacc cgatctccac cttcggagat tcgctcgcgc ttggggaatc
      121 gcatattcgc gctgcatcgg cgaattcgct aatcgttcgc atcgccgagc tatgccatcc
      181 ggatgcatgg ttcatcggcg gcgccgtgcg cagcctgtgt gccggaaaaa gggtaaccgt
      241 cgtccgtgac tggcatgttt cgccgacatc cctctctgat tgcgcaaacg cgctggttgg
      301 gctcttgttg aaggaccatc atggaatcgt gaatgtatca tgcgcgggaa gcgacaccat
      361 gcgatcggtc atcgagagag ccgccggacg cgtttccgac ctacgtggcg tcgatgcaac
      421 gtcgatgatc gacgagatcg atgccgttga gtttgtcacg ccgtatgatt tcccgctatc
      481 ggccgttctg gacatttcgc tcttgtcccg tgtcgcttca tgcaatcctc gtccctggca
      541 agaggcgctt cgagaatgcg tagaggcgat aatcgcgtca ggacaataat cgcttgacga
      601 ggtagcaccc ggcgagaagc agcacgagcg taatcagcac caccgccttt ttccaatctt
      661 cgaagtccct cccacgctca agcatgatga agacatgggt cgagagcgtt gccgtaattg
      721 acatgaacat ggcgtacaaa accaacgaaa gtgaaaaaag accgatcccg acgaagaggc
      781 ctgcaatctg atggacagcc ccagcgttag agtggaacga tcgcagaagg ccctgaccgc
      841 gtccggttat tgaaatgtcg aatacgcgca agaggtccga cggaatggcg gaaaatacgg
      901 ctgcgccttc atgaggaatg atcgattcgg taagaaacgc ggtcagggaa aatgcgccca
      961 catgaacgta gtacaggacg ccgacgacca gcaatccggt aaatgagagt atgggaatat
     1021 aaaaaagcaa attgaaatta tgacgacgga tgaaatggaa caggttgatc gcgttttccg
     1081 ctattccgga cttccgatat gcgacaagcg gcgggtaaaa ccagaatccg ataatcgcga
     1141 caatgatgat gcctagcgac agcaggtata cgggcaggaa tatcagcgag tagaaaaacg
     1201 ggccgatcag cggaatatgc ccgaacacga tgaagagggc gcctgcggaa aacagcacca
     1261 ggacattggt gatgcaaatc gacggaaggg ctcgaatgat aaatcgcggc aatgaccccg
     1321 cgtcggcata gttctgccgg aagaggtcct tgatggtgat ccgcgaaatg accgcggcga
     1381 tggtgacata ggcgacgaaa acgatcagaa tcgggaccaa ctggaggact ccgcgaatcg
     1441 acgcatgggt tttcggcacc acatgcatta cgacaagcgt atcgatgcgc gcatagacaa
     1501 tgagcagcaa tgcaaggagg acagtcccga ttacaccagc ccctattttt ggaagcgtaa
     1561 aagaagacgc gcagacggta aatatctcgc gtagggtgaa atcacggaac gtgtccacag
     1621 agaataccgg ttccgtgttg cgagaaaccg gtgtatcgac acgcctcttc gcggtttttt
     1681 ccgatggatc ggccggcttt tttcgaaaaa tggatacaac ggtcaccaac cgacctattt
     1741 tttgcgcaat ggtcccatca tttttcttcg gtcgtgttgc tggagcttgt gctcgctgcg
     1801 tcgtggcatc aaccgccacg ggcgcgacgg tcgggaaatt gacaacgaat tgtttcccgc
     1861 atctcctgca ggtaaggcga tactgcgggc tggaagccgt ggtaaacgta atcgagtaca
     1921 gacaattgca atgctggcaa aatgcgattt taatatactt cccgcagctt ctgcactgga
     1981 agatcgaatg acgcaatccg ctcacctgcg catcggtgag cgtatactgg gaatcgcatg
     2041 ttgaacaggt tatctgcata cttagtcgtc cagcatctca aggttcaggg attctatgtc
     2101 ctcacggctg ttctttcccg tttcacgttc aatcattcgc gcctttcgtt tgcgcgccag
     2161 ttcgaatacg ctatcgggat catcgtgctc gagtgagcat gagtccgttg gctgcgtacc
     2221 ctcaagaaaa atctccgtgc ggctcgcgca tccggggacc gggagcattc ccgtcttcgt
     2281 gcatacggtg caatatgtta tgcccttcgg gcgacgcggg aagggcgctg gttttcgtgt
     2341 cgcatagatc gacttcatgt acgatcccca aatcggggcc gcggcaacgg cgccgctctg
     2401 tccgtggcca agggtgaact tcggcgaatc gcatccgacc caaaccgccg ttaccatgtc
     2461 cgcggtatat ccgacgaacc acgcgtcctt gaagtcggag ttggttccgg tttttcctgc
     2521 ggccgggaga cgaaatccgg cggcgcgtcg aatcggccca tgaccggtcc cggaatcgac
     2581 gacactgcgg agaaggtcgg tgaggatata tgaaacgccc ggatgtacaa gctgtttttt
     2641 atgaattttt tcgatttcgg cttccgtatc attgatgcgt tttccgtgcc ggtcgagtat
     2701 ataacggata cagtacggac gcaccgattt cccgccattt gcgaacacgg cgaatccgag
     2761 cgctatctcg tatggagaca cctcggtggt tccgagcgcg agcgtcggat ttacatcgaa
     2821 ccgattgtcg gggagtccca tcaggtgacc ggaaaatttc gcgatgcgat caccgccgat
     2881 ctcatcgtat gccaggaccg agacgacatt cagcgatgag ctgagcgcct ttcgcacgag
     2941 tactttgccg ctataggatt tattgtagtt tgtcggggtc cagctctttc gaacgccctc
     3001 gaaggcggtc ggcgaatcca aaaagg
//
LOCUS       DAORHF010000372         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_37561_length_3020_cov_5.309612, whole genome shotgun sequence.
ACCESSION   DAORHF010000372 DAORHF010000000
VERSION     DAORHF010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37561_length_3020_cov_5.309612"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(297..1319)
                     /gene="aroF"
                     /locus_tag="PKJ16_18540"
     CDS             complement(297..1319)
                     /gene="aroF"
                     /locus_tag="PKJ16_18540"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HNV49046.1"
                     /translation="MVLVLNEPESPHALKAKADCATRGCRAYDLATSRGTVSAVLGLG
                     SVDLERMAGESTSLSVVTPKSPYILAGRAIKDTDTVVTIRERAQIGGGPLAVIAGPCS
                     IESEAQIMETAERVRAAGAAILRGGAFKPRTSPYAFQGMGLEGLRLLRRAGDAVGLPI
                     ITEVLDADDIGPVAELADIIQIGARNCQNFSLLRKIGRVGRPVLLKRGMMVTIEEFLM
                     SAEYLLSEGNMNVILCERGIRTFETETRNTLDISAVPILKAKTHLPVIVDPSHAAGTW
                     TLIRPLALAAVAAGADGLMIEVHSSPETALCDGGQSLRPYRFDELMSALAPLAGAMGR
                     AIAGSA"
     gene            1758..2072
                     /locus_tag="PKJ16_18545"
     CDS             1758..2072
                     /locus_tag="PKJ16_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49047.1"
                     /translation="MAVIADSVCLENSLIDDVCRRELMAMDRHEIIVKLCDVIGPCPS
                     NLSKAEELFETLRYEIANYVLHHDYKSSRGVSADLLLWLDGQCNPDQAQAFRALMGRF
                     IK"
     gene            2076..2684
                     /locus_tag="PKJ16_18550"
     CDS             2076..2684
                     /locus_tag="PKJ16_18550"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HNV49048.1"
                     /translation="MTVHRLLLHACCAPCAAHVCSLLAREHDVVVYYYNPNIAPRSEY
                     DRRLDELDRYARSEAFPLVVGGYDAREWTRAVRGLRHQGERSARCGACFRFRLAGVFR
                     YARENGFAAVATVLSVSPHKDAALINEVGRSLAAESGIGFIEADFKKHDGFRRATALS
                     RERGFYRQNYCGCIYSKLERDRSSTWFRKYCADRGATRIVPR"
     gene            2764..>3020
                     /locus_tag="PKJ16_18555"
     CDS             2764..>3020
                     /locus_tag="PKJ16_18555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49049.1"
                     /translation="MTPESEYDQDGLLRAARRLRFPSFLESSFLDYYHNKTILFIRWA
                     SVFGILLYLANLNTDFISFPGLERLAIGVRVGFVVYGVIILA"
BASE COUNT          531 a          937 c          950 g          602 t
ORIGIN      
        1 acgaggaaca tgatgatttt cgctttgatg ctcatggagc gcccccggcg tttcatttcg
       61 aacaaccggt gctttgctat ggaagaatcg cgcatctgtc catcatttta ttggctggac
      121 gggaatcggc gccggcgcgt ccgcacgggg gccgtgcgac cctgactcga agacccgcag
      181 ctgcggaacg acaggcccgt ggcgtcccgc ccgccagtgc agtgcggccg ggaaaccgct
      241 cggagcggcc gtgaacgcac tgtcgatgtc gcgctcttcg aattgggccg atccgcctac
      301 gcgctcccgg caatcgcccg gcccatcgcg cccgcaagcg gggccagcgc gctcatgagc
      361 tcgtcgaagc gatagggcct gagggactgg ccgccgtcgc acagggccgt ctccgggctg
      421 ctgtgcacct cgatcatgag gccgtcggcc cccgcggcca cggcggccag cgcgagcggg
      481 cggatgagcg tccacgtccc cgccgcgtgc gacgggtcca cgatcaccgg caggtgcgtc
      541 ttggccttga ggatcggcac cgccgagatg tccagcgtgt tccgcgtctc cgtctcgaag
      601 gtgcggatgc cgcgctcgca gaggatcacg ttcatgtttc cctcggagag caggtactcg
      661 gccgacatga gaaattcctc gatcgtgacc atcatccccc gtttgaggag gaccgggcgg
      721 ccgacgcgtc caattttcct gagcagggag aagttctggc agttgcgcgc gccaatttgt
      781 atgatatcgg cgagctccgc gacggggccg atgtcgtccg cgtccagcac ctcggtgatg
      841 atgggaagtc ccacggcgtc ccccgcgcgg cgcaggagcc gcaggccctc gaggcccatt
      901 ccctggaagg catagggcga tgtgcggggc ttgaaggcgc ccccgcgcag aatggcggcg
      961 cccgcggcgc ggacgcgctc ggcggtttcc atgatctgcg cctcgctctc gatggagcag
     1021 ggccctgcga tcaccgccag cggtccgccg ccgatctgcg cgcgctcgcg gatcgtcacg
     1081 accgtgtccg tgtccttgat ggcgcgcccg gccaggatat agggcgattt cggcgttacg
     1141 acggacagcg aggtcgattc gcccgccatc cgttcgagat cgaccgatcc cagccccaga
     1201 acggcgctta ccgtcccgcg gctggtggcc agatcgtagg cgcggcaccc ccgcgtggcg
     1261 caatcggcct tcgccttgag cgcgtgggga ctctcgggtt cgttgagtac gagtaccatg
     1321 gtgtacctcc ggtcgtttgg gtttgcgggg ctttggggag gttggcgacc ggggtgggtc
     1381 ctgaaacgaa gaaggccgcc gacttgtcgt ccgcggcctt cgcgcaatgc gtcatcagca
     1441 aaagctacgg acagctttta aaataaaagt accaaaaata aaagcagctg ctgatggcgg
     1501 caatcttcga attcatgatg gtgccatctt tcatgacccc gggtgtctgt caactgaata
     1561 atgcatgatc cggggcatta aggacaaaaa gacggttttt ttggaaaatt gttccgcggc
     1621 ggttgacgct tgggcgcggt atgtatatat tctcaatata tacaaatggt taaaactata
     1681 aggaatattt tttctattaa aaacagggtg ctattgccgc tatgatgaac aaacctgccg
     1741 cgaccctcga gcccgaaatg gctgttatcg ccgattcagt gtgcctcgaa aattccctca
     1801 ttgacgatgt ctgccgccgc gaactcatgg ccatggaccg acacgagatc atcgtgaaac
     1861 tgtgcgatgt catcggcccg tgcccctcga acctgtcgaa ggccgaggag ctgttcgaaa
     1921 cgctgcgata cgagatcgcg aattacgtgc tccatcacga ctacaaatcg tcgcgcgggg
     1981 tgagcgcgga ccttctgctg tggctcgatg gccagtgcaa ccccgatcag gcacaggcct
     2041 tccgcgcgct gatgggtcgc ttcatcaagt aggccttgac cgtccacaga ctcctattgc
     2101 acgcctgctg cgctccatgc gcggcccatg tctgctcgct cctcgcgcgg gagcacgacg
     2161 tggtcgtcta ttactacaat cccaacatcg ctccccgttc cgagtacgac cggcggctgg
     2221 atgagctcga ccggtacgcc cgttccgagg ccttcccgct cgtcgtgggg ggatacgatg
     2281 cgcgcgagtg gacgcgcgcg gtgcgcgggc tgcgccatca gggtgagcgc tcggcccggt
     2341 gcggggcctg ctttcgattt cgccttgccg gggttttccg ctatgcgcgc gagaacggct
     2401 tcgcggccgt cgccaccgtc ctgtcggtga gcccgcacaa ggacgccgcg ctcatcaacg
     2461 aggtcggccg ttcgctcgcg gcggagagcg gcatcggctt catcgaggcg gacttcaaga
     2521 agcatgacgg atttcgccgc gcgacggcgc tctcgcgtga gcgggggttt taccggcaga
     2581 attactgcgg atgcatctac tcgaagctcg aacgcgatcg aagctcgacc tggttccgga
     2641 agtattgtgc ggaccggggc gcgacgcgga tcgtcccgcg atgaagtgca aatgatcttg
     2701 actttttccc gttccggtga cttctgaggc gcagaaacac agaaagagcg cgtcgcgatg
     2761 ccaatgaccc ccgaatccga atacgaccag gacggactgc tgcgagccgc acgccgattg
     2821 cgctttccgt cgttcctcga gtcgtcgttc ctcgactatt atcacaacaa gaccattctg
     2881 ttcatccgat gggccagcgt gttcggcatc ctgctgtatc tggcgaacct caataccgat
     2941 ttcatctcgt tcccgggcct ggagcgcctc gcgatcgggg tgcgcgtcgg gttcgtcgtc
     3001 tacggcgtga ttatcctggc
//
LOCUS       DAORHF010000373         3019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_37591_length_3019_cov_3.431511, whole genome shotgun sequence.
ACCESSION   DAORHF010000373 DAORHF010000000
VERSION     DAORHF010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37591_length_3019_cov_3.431511"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PKJ16_18560"
     CDS             complement(<1..407)
                     /locus_tag="PKJ16_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HNV49050.1"
                     /translation="MALKSLLKGFKRPNKISFEHDELKKDYGRFVAEPFEKGYGLTLG
                     NSLRRVLLSSIEGAAITAIKIEDVPHEFHAIDGVYEDVARIILNLKKLRFRYHGELQK
                     VLHIVKKGPGDLTGQDFNIDSDVEVMNPELVIAT"
     gene            complement(482..1111)
                     /gene="rpsD"
                     /locus_tag="PKJ16_18565"
     CDS             complement(482..1111)
                     /gene="rpsD"
                     /locus_tag="PKJ16_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HNV49051.1"
                     /translation="MGRYTGPSCKLCRREGVPLMLKGQRCLTDKCAVKKKKYLPGPPR
                     KRRSKLSEYGIQLREKQKIKKSYGLLEKQFKLIFEEASRMKGVTGHNMLSLLERRLDN
                     VVYRMGFATSRKQARQFVQHRHVLVNDRIVDIPSFVVREGFEVRIGDSFKESAMLTDA
                     VKLSKAISSVPEWINADFENKSGKVVRLPNRQDVTANINEQLVVELYSK"
     gene            complement(1124..1513)
                     /gene="rpsK"
                     /locus_tag="PKJ16_18570"
     CDS             complement(1124..1513)
                     /gene="rpsK"
                     /locus_tag="PKJ16_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HNV49052.1"
                     /translation="MKQKGQKKKKEKKIVPIGRVCIQASYNNTIITITDMEGQVISWA
                     SAGGEGFRGSRKSTPFAAQTAAKTAVGKAIDIAGLRSVEVFVKGPGIGREAAIRSLAQ
                     AGLAVTKIKDVTPIPHNGCRPPKRRRV"
     gene            complement(1530..1904)
                     /gene="rpsM"
                     /locus_tag="PKJ16_18575"
     CDS             complement(1530..1904)
                     /gene="rpsM"
                     /locus_tag="PKJ16_18575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HNV49053.1"
                     /translation="MARIAGIDLPNNKRIEVALTYIFGIGPSTSHKILAETGINPDVR
                     VKDLRDDEINSIKKLIEQSYKVEGDLRTEVAMNIKRLMDIGCYRGIRHRRGLPVRGQN
                     TKNNAHTRKGNRKSAQAKKKKA"
     gene            complement(1926..2057)
                     /gene="rpmJ"
                     /locus_tag="PKJ16_18580"
     CDS             complement(1926..2057)
                     /gene="rpmJ"
                     /locus_tag="PKJ16_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005771506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HNV49054.1"
                     /translation="MKVRVSVKKVCSDCKIIKRHGVVRVICKNARHKQRQKVRTASK"
     gene            complement(2096..2317)
                     /gene="infA"
                     /locus_tag="PKJ16_18585"
     CDS             complement(2096..2317)
                     /gene="infA"
                     /locus_tag="PKJ16_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041278396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HNV49055.1"
                     /translation="MAKEEPIVVEGVVIEPLPNAMFRVMLDNGHKILAHISGKMRMHY
                     IRILPGDRVTVELSPYDLSRGRITYRSKN"
     gene            complement(2330..3004)
                     /gene="map"
                     /locus_tag="PKJ16_18590"
     CDS             complement(2330..3004)
                     /gene="map"
                     /locus_tag="PKJ16_18590"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HNV49056.1"
                     /translation="MDVGVTTYELDRFARDIIAKHRARPAFLGYKGFTAALCTSINEV
                     VVHGIPSKSARLKSGDIVGIDVGVFLDGYYGDTARSFAIGAISKEARQLMDVTRASLY
                     AGIDRCVVGNRISDVSHAIEAYVVQYGFLPVREFVGHGIGRSLHEEPPIPNHGKPGRG
                     PRIKNGMVLAIEPMINVGTYLVDVDQKDGWTVYTKDRKLSAHFEHTVAVVNGRAEILT
                     KGNNFS"
BASE COUNT          608 a          959 c          703 g          749 t
ORIGIN      
        1 gtcgcgatca ccagctcggg attcatcacc tcgacatcgg aatcaatgtt aaaatcctgc
       61 cccgtgaggt cgccgggacc tttcttgacg atatgaagaa ccttttgaag ctcgccatga
      121 taccgaaacc ggagcttctt caggtttaag ataatccgag cgacatcctc gtagactccg
      181 tcgattgcgt ggaactcgtg cggcacatcc tctatcttga tcgccgtgat cgccgcaccc
      241 tcgatcgacg agagcaacac gcgacgaagc gaattgccca gcgtgaggcc gtatcctttc
      301 tcgaagggct ccgcgacaaa ccgcccgtaa tcctttttca actcgtcgtg ttcaaaggat
      361 attttattgg gtctcttgaa cccttttaag aggcttttta gagccatccg tccctccaca
      421 aaatcttcat catcccagac tgactggccg ttgcgcggac tcgtttccgc cgcatcgacc
      481 gttacttcga atagagttcc accacgagct gctcgttaat gttcgcggta acatcctgcc
      541 gattcggcaa ccggaccact ttgcccgatt tattttcgaa atcggcgttg atccactccg
      601 ggaccgagct gatcgccttc gacagcttta ccgcatcggt cagcatggcg ctctccttaa
      661 aggaatcgcc gatacgcacc tcgaatccct cacgaaccac aaacgaggga atgtcgacga
      721 tgcgatcatt caccaggaca tgccggtgct ggacaaactg ccgcgcctgc ttgcgcgatg
      781 tcgcgaaccc catgcgatac accacattgt caagccgtcg ctcgaggagc gagagcatgt
      841 tgtggccggt cacgcccttc attcgagagg cttcctcgaa aatcagctta aactgcttct
      901 caagcagtcc atagctcttt ttgatcttct gcttctcccg cagctgaatg ccgtattcgg
      961 agagtttcga ccgccgcttt cggggcggcc caggcagata ttttttcttc ttcacggcgc
     1021 acttgtccgt caggcagcgc tgccccttca gcatcaacgg cacgccctcg cgccggcata
     1081 gtttacacga tggtccggta tatcttccca tgctctcccg tcctcacact ctgcgtcgtt
     1141 ttggcggacg acagccgtta tgtggaatcg gcgtaacgtc cttgatcttg gtgaccgcca
     1201 ggcccgcctg tgccagcgac cggatagcgg cctcacggcc gatgccgggt cccttcacga
     1261 acacctcgac actcctgagc cccgcgatat cgatggcctt gcccacggcg gtctttgccg
     1321 cggtctgcgc ggcaaacggc gtcgacttcc gggagccccg gaacccctca ccgccggcgg
     1381 acgcccacga tatcacctgg ccctccatgt cggttatggt gatgatcgta ttattatagc
     1441 tcgcctgaat gcagaccctc ccgatgggga cgatcttctt ttctttcttt ttcttttgcc
     1501 ctttctgctt cacgaatcgt cactccctct tatgcttttt tcttctttgc ctgcgcggat
     1561 ttgcgattgc ccttccgcgt atgggcgttg ttcttcgtgt tctggccgcg aaccggcaga
     1621 ccgcggcgat gtcgaatccc tcgatagcag ccgatatcca tgagccgctt gatgttcatc
     1681 gcgacctcgg ttcgcagatc gccctccacc ttgtagctct gttcgatgag cttcttgatg
     1741 gagttgatct cgtcgtcccg aagatccttc acccgcacgt caggattgat gccggtctcg
     1801 gcgagtatct tgtgcgatgt gctcggtccg atgccgaaga tataggtcaa cgcgacctct
     1861 atccgcttat tgttcggtaa atcgatacct gctatacggg ccatatccca ttaacctctg
     1921 aagagttatt tacttgcggt ccggaccttc tgccgctgct tatgacgcgc gttcttgcag
     1981 ataacgcgca ccacgccgtg ccgcttgatg attttacaat cgctgcacac ctttttaact
     2041 gacactcgca ccttcatcac aaaccccaca caacagcaat attgatccgc ctgccctagt
     2101 tcttggagcg atacgttatt cggcccctgg acagatcgta tggcgaaagc tcaaccgtca
     2161 cccggtctcc cggcagaatg cggatgtagt gcattcgcat cttccccgat atatgagcca
     2221 atattttatg gccattatcg agcatcaccc ggaacatcgc atttggcaac ggctcaataa
     2281 caacgccctc tacaacaatt ggctcttcct tcgccatact cccgctcgat cagctgaaat
     2341 tattcccttt tgtcagaatc tccgcccggc cattgacaac ggcaaccgtg tgttcaaaat
     2401 gcgcagagag ctttcgatcc ttcgtataga cggtccaccc gtccttctgg tcaacatcca
     2461 ccaaatacgt gccaacgttt atcatcggct caatcgccaa caccatgccg tttttgatgc
     2521 gcggcccgcg tcccggcttt ccatgattgg ggatgggcgg ctcttcgtga agcgaccgcc
     2581 cgatcccatg cccgacaaac tcacggaccg gcagaaatcc atattgaaca acatacgcct
     2641 cgatcgcatg cgatacgtcg gaaattcggt ttcccaccac gcaccgatca ataccggcat
     2701 acagcgacgc cctggttacg tccatgagct gccgcgcttc tttggatatg gcgccgatcg
     2761 cgaaactccg cgcggtatcg ccgtaatatc cgtccaaaaa caccccaacg tctataccca
     2821 cgatatcgcc gctttttaat cgcgcgcttt tgctgggtat tccatgcacc acaacctcgt
     2881 ttatcgaggt gcacagcgcg gctgtaaatc ccttgtaccc taaaaacgcc ggccgtgccc
     2941 ggtgcttcgc aatgatgtcg cgcgcaaacc ggtcaagctc atacgtggtt acgcccacat
     3001 cgatgatccg gtcaatttc
//
LOCUS       DAORHF010000374         2998 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_37965_length_2998_cov_6.002039, whole genome shotgun sequence.
ACCESSION   DAORHF010000374 DAORHF010000000
VERSION     DAORHF010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37965_length_2998_cov_6.002039"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="PKJ16_18595"
     CDS             <1..1784
                     /locus_tag="PKJ16_18595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49057.1"
                     /translation="WCLDETALRSLPVSDTLAGHLGERLTSLPAPTSRLLAAAAMIGG
                     DFHARLLSRATGFSEEELSRNLGELAQEGYIRETDGGAWRIAHDRILEAASALVDADD
                     AARLHLAIGRALLAELGDDPGDLLFAVADHLNRAGTPVGADEAVRLAEINLRAGERAM
                     SSGAFESARSFFRCGMDAIGLPGAGEAWRTRYDLSFALCRHRAETEYLCGDHDAFDAV
                     LQSSLNLVSDPLDRTELEMLRIQQLAMAARHAEAIQHGINALHSLGIEFPNENFRAAV
                     AGELAAVEAYMKGRSIASIFDNPEMSDPAYRNSTKIMSIMLNSAFHCDPVLFRLILLK
                     IISLSIKHGNNAHSCAGFIGYGLILTNVFFDFVNGFEYGLLGVRLVERFPELQIRCSA
                     LHQFTIHILPWNKHLRETVPYNANAYQAGIESGELYFANINKHHTMASRYHAGESLDA
                     LRDDFIACLAQMHAQRQYVTLEPALNYIGFLANLTGETGSADCFDTEYITDDQHARNM
                     TTENARTSLCQYYMLRAQALHLYGRCEQALPFAERALLNQQHMMGCFHVAELNCSHSL
                     ILAALCDSADGDRRPAFSGRSARTSKR"
     gene            1781..>2998
                     /locus_tag="PKJ16_18600"
     CDS             1781..>2998
                     /locus_tag="PKJ16_18600"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HNV49058.1"
                     /translation="MKKWSDNCPSNFLHKYLLVEAELARLDGNNAVATDLYDAAIESA
                     GANGFVQNEAIANECAGRFWLARGKHDFAALYLERARRGYARWGARRKVWEMNAQYPD
                     ILAPVGGDRAPHADTPTIPVEPMPANVDLEAVFRASTALSQEIDLDRLLERVVDIILR
                     NSGARRMALAIEHDGEYFIEAEGRFDDERSGVRTKLVRSVPYADYPGLPVSVISYVAR
                     TGEPVWLDNAAAEGLFVADPYIRDGRIASVACNKLRYGKTATIGYLENADTIGAFTPE
                     RMGVVNLLARQVGISLDNARLYRTLEESNARLRELDAVRDRLLRQYEESRQKNLQDRM
                     RPHFLYNAINTVHSLIATDPARADEALMTLSGLFRYLTDVSFEPLVPFSEAWGFTESY
                     LEFEKLRFPRFLTF"
BASE COUNT          568 a          999 c          900 g          531 t
ORIGIN      
        1 gctggtgcct cgacgagacg gcgcttcgct cccttcccgt ttcggacacc ctggcggggc
       61 atctcggcga acggctcacg agcctcccgg cgccgaccag ccggctgctt gccgcggcgg
      121 cgatgatcgg cggcgatttt cacgcacgac tcctttcccg agccaccggc tttagcgagg
      181 aagagctatc gcgcaatctc ggcgaactgg cccaggaagg ctacatcagg gagacggatg
      241 gcggcgcctg gcggatcgcc cacgaccgca ttctcgaggc ggcgtcggcc cttgtcgatg
      301 cggatgacgc ggcgcggctg cacctggcga tcgggcgtgc cctgctcgcg gaactcggcg
      361 acgatccggg cgatcttctg ttcgctgtcg cggaccatct caaccgcgcc gggacgcccg
      421 tcggcgccga cgaggccgtc cgtctggccg agatcaatct gcgggcggga gagcgcgcga
      481 tgtcgtcagg ggcattcgag tcggcgcgct cgttcttcag gtgcggaatg gacgcgatcg
      541 gcctcccggg ggccggcgag gcatggcgga cccgttacga cctgtcgttc gccctgtgcc
      601 gacatcgcgc cgagacggaa tacctgtgcg gcgatcacga cgccttcgac gccgtgctgc
      661 agtcgtcgct caacttagtg tcggacccgc tggaccgcac ggagctggag atgttgcgca
      721 tccagcagct cgccatggcg gcgcggcacg ccgaggcgat acaacatggg atcaatgcat
      781 tgcattccct gggaatcgag tttccgaatg aaaacttccg agcggccgtc gccggagaac
      841 tggcagccgt cgaggcatac atgaaaggcc gatcgatcgc ctcgatcttc gacaatcccg
      901 agatgtccga cccggcatat cgcaacagca ccaagatcat gagtatcatg ctcaatagcg
      961 cgtttcattg cgatcccgtt cttttccgtc tcattctgtt gaaaatcatt tcactttcca
     1021 taaaacatgg caacaatgcg cactcgtgcg caggttttat cgggtatgga ctcatcctca
     1081 caaatgtttt ctttgatttc gtcaatggat tcgagtacgg acttctgggc gtccgacttg
     1141 tcgaacggtt cccggaacta cagatccgat gttcagcgtt acaccagttc accatccaca
     1201 ttctgccatg gaacaagcat ctcagggaga ccgtacccta taacgcgaat gcctaccagg
     1261 cgggcataga gtccggcgaa ttatactttg ccaatataaa caagcaccac accatggcaa
     1321 gccgctacca cgcgggcgaa tccctcgacg cgctgcggga cgacttcatc gcgtgcctcg
     1381 cccagatgca cgcccagcga cagtacgtta ccctggagcc agcgctaaac tacatcggct
     1441 tcctcgcaaa cctcaccggg gagaccggat cggcagattg tttcgacacg gaatacatca
     1501 ccgacgacca gcacgcgcga aacatgacga cggagaacgc ccggacatcg ttgtgccagt
     1561 actacatgct gcgggcgcag gcgctgcatc tgtacgggag atgcgagcag gcgctcccgt
     1621 tcgccgagcg cgccctgtta aaccagcagc acatgatggg atgtttccac gtcgccgagc
     1681 tcaactgctc gcactcgctc atactcgccg cgttatgcga cagcgccgac ggggaccgcc
     1741 ggccggcttt ctcgggcaga tccgcgagaa ccagcaaacg atgaagaaat ggtccgataa
     1801 ctgcccgtcg aacttcctcc acaagtacct cctcgtcgag gccgagctcg cgcgcctcga
     1861 tggaaacaat gccgtcgcca cggacctcta cgacgcggcg atcgagtcgg ccggcgcgaa
     1921 cggattcgtc cagaacgagg cgatcgcgaa cgagtgcgcc gggcgcttct ggctcgcgcg
     1981 gggcaagcac gacttcgccg ccctctatct cgagcgggcg cggcggggat acgcccggtg
     2041 gggcgcgcgc cgcaaggtgt gggagatgaa cgcacagtat cccgatatcc tcgccccggt
     2101 cggcggggac cgggccccgc acgccgacac gccgacgatc cccgtcgaac ccatgccggc
     2161 caacgtcgac ctcgaggcgg tgttccgcgc ctcgacagcg ctttcccagg agatcgacct
     2221 cgaccggttg ctcgagcggg tcgtcgacat catcttacgg aactccgggg cgcgtcggat
     2281 ggccctcgcc atcgagcacg atggcgagta tttcatcgag gcggagggac gcttcgacga
     2341 cgaacggtcc ggcgtccgga cgaagctggt ccgctcggtg ccgtacgccg actatccagg
     2401 actcccggta tcggtaatca gctacgtggc ccgtacggga gaacccgtgt ggctggacaa
     2461 cgcggcggcc gaggggctgt tcgtcgcgga cccctacatc cgggatggcc gcatcgcctc
     2521 cgtcgcctgc aacaagctcc gttacggaaa gaccgccacc atcgggtacc tggagaacgc
     2581 ggacacgatc ggcgccttca cccccgagcg catgggcgtg gtaaacctcc tcgcccgaca
     2641 ggtgggcatc tcgctggaca atgcgcgcct ctaccgcacg ctcgaggagt cgaacgcgcg
     2701 gctgcgcgag cttgacgcgg tgcgcgatcg cctgctccgt caatacgaag aatcgcgcca
     2761 gaagaacctg caggaccgca tgcgcccgca ttttctctac aacgcgatca acaccgtcca
     2821 ttcgctcatc gccacggacc cggcccgcgc cgacgaagcg ctcatgacgc tttcgggcct
     2881 cttccgatac ctcaccgacg tttcctttga gccgctcgtt ccgttcagcg aggcatgggg
     2941 cttcaccgag agctacctcg agttcgaaaa actgcgcttc ccgcgctttc tcacgttc
//
LOCUS       DAORHF010000375         2957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_38785_length_2957_cov_4.911440, whole genome shotgun sequence.
ACCESSION   DAORHF010000375 DAORHF010000000
VERSION     DAORHF010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2957
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38785_length_2957_cov_4.911440"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1659)
                     /locus_tag="PKJ16_18605"
     CDS             complement(127..1659)
                     /locus_tag="PKJ16_18605"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HNV49059.1"
                     /translation="MSAVHPHTEQRILLFSVFILSLCGIVYELVLGSLATYLLGNPVQ
                     QYSITIGIFLSAMGLGSYLSRYITKDLVRNFILIEAVLGLVGGVSVTVLSVFFTYLLS
                     FYALQFFFLIGIGVLVGLEIPLVTRILKKHGSLRDIISNVLTFDYVGGLIGSLLFPLV
                     LFPFVGRYLTSVIVGSVNIAVALFLVVVIEYPGKRRWHVVYPISCLIIMGVLAGASGK
                     IGHLLQRNLYADDIVFSKRSSFQEIVLTRSGDDFRLFLDGALQFSTFDEYRYHEMLVF
                     PALLMRETDPKSVLVMGGGDGLAVREIKKMRTVQLIHLVELDPEMIRLSLRNPSFQKI
                     NDLALHDPRVRITVGDAHRFLMEHRRLFDVIIADFPDPHDETIAKLYTEEFFRLVAKN
                     LAPDGVFVTQSTSPLFAREAFWCIHNTMKRVFPSVVPYRVYVPTFGEWGFNIGSKKTL
                     GEIRLREHDTAVDARYYTRTTFMNALHFAQDNGPVDTRINTFNTPVLYSYYVRGWKSH
                     DL"
     gene            complement(1656..1943)
                     /locus_tag="PKJ16_18610"
     CDS             complement(1656..1943)
                     /locus_tag="PKJ16_18610"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HNV49060.1"
                     /translation="MDWGSVGIGIIETVIYSLVGIAVMGIGFLLFKLICPFSIKKEIE
                     DDQNTSLGIIIGSIIIGLSIIVASVISTPTSSKTRPVKADAPVATDIEAKK"
     gene            complement(2022..2213)
                     /locus_tag="PKJ16_18615"
     CDS             complement(2022..2213)
                     /locus_tag="PKJ16_18615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49061.1"
                     /translation="MWRAIVVVVLALGGVYWMSSQFVGYNRAGNYYIGKQRTERKSVR
                     VGSAGYYGRGRPGGIRTGK"
     gene            complement(2235..>2957)
                     /locus_tag="PKJ16_18620"
     CDS             complement(2235..>2957)
                     /locus_tag="PKJ16_18620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49062.1"
                     /translation="YEKTRRKRSAYAWRARIYGIGIVAAIIYIIYAAVSGDSLPDISK
                     TRRVLVENEVKVEQGKQVYASQILFGPAVFQRSNSLYTVRVAVDSAVQPLRLEWQSFR
                     LLLIKEDRIRQFESRQAGADGIRTALAEIDEFEEPVESYAFNGDFWDEEGYDDEGKWH
                     ESDTSGETDFILDDPGSYYLYVELFSEKPRKSGAIAISILENVKGFRYFFIPIVLFSV
                     LLLVNHYRGKNYNELPFEMANH"
BASE COUNT          722 a          840 c          756 g          639 t
ORIGIN      
        1 gctgaaggat atcggcatcg cattcggcat cgatcgcaat atccgactgt ccttcgccac
       61 gtcagatgat aacatcatcg aaggcgtcag ccgcatgaag gaggtcgtcg gccggctcat
      121 atgatcctag aggtcgtgtg acttccatcc gcgcacatag tacgaataca gcaccggcgt
      181 gttaaacgtg ttgattctcg tatcgaccgg tccgttgtcc tgggcgaagt gcagcgcatt
      241 catgaacgta gttctggtgt aataccgcgc atcgacggca gtgtcgtgct ccctcagacg
      301 aatttcgcca agcgtctttt tcgatccgat attaaacccc cactcgccga aggtcgggac
      361 gtaaacgcga tacggcacca cgctcgggaa cacgcgcttc atcgtattgt gtatgcacca
      421 gaaagcctct cgcgcgaaga gcggcgacgt cgattgcgtt acaaatacgc cgtccggcgc
      481 gaggtttttc gcgaccaatc gaaaaaactc ctcagtatac agcttcgcga tcgtctcgtc
      541 atggggatcc ggaaaatccg cgatgatgac atcgaagagc ctgcggtgct ccatgaggaa
      601 ccgatgcgca tctcccacgg tgatgcgaac ccggggatca tgaagcgcta aatcgttgat
      661 tttctggaat gacggatttc gcagggaaag gcgaatcatc tcgggatcga gctcgacgag
      721 atgtatcaat tgcacggtac gcattttctt tatctcgcgt acggcaagcc cgtcgccgcc
      781 gcccatcacc aggacgcttt tcggatcggt ttcgcgcatc aagagtgcgg gaaagacgag
      841 catctcgtga tagcggtact cgtcgaaggt cgagaattgc agcgcgccgt caaggaagag
      901 ccgaaagtcg tccccgctcc tcgtgagcac aatctcctga aaggacgacc gtttcgaaaa
      961 cacgatatcg tcggcataca ggttccgctg gaggaggtgt ccgatcttcc cggacgcacc
     1021 cgcgagaacc cccataatta taaggcatga gatcggatat acgacatgcc atcgtctttt
     1081 tccgggatac tcgataacga cgaccagaaa taacgccacc gcgatattca ctgaaccgac
     1141 gatcacgctg gtgagatatc gtcccacgaa cgggaatagg accaacggga acagcaggga
     1201 cccgatgagg ccgccgacat agtcgaaggt gagcacattc gagatgatgt cgcgaagcga
     1261 tccgtgtttt ttcaggattc gcgtcaccag cggaatctca agacccacca agacgccgat
     1321 cccgataagg aaaaaaaact gcagcgcgta aaaggaaagc aggtaggtga agaatacgct
     1381 gaggacggtg acggacaccc ccccgaccag gcccagcacc gcttcaatga gtatgaaatt
     1441 cctgacgaga tccttggtga tgtatcgcga gagatacgat ccgagtccca tcgcgcttaa
     1501 aaaaattccg atggtgatcg aatactgctg aaccggattc cccagcaagt acgtcgccag
     1561 ggaacccagc acgagctcgt agacaatacc gcagagcgag agaatgaata ccgaaaagag
     1621 cagtatccgc tgttcggtat gcggatgtac ggcgctcact ttttcgcttc gatatcggtt
     1681 gcaacgggcg catcggcctt aaccggccgt gttttcgagc tcgtcggcgt cgaaataacc
     1741 gatgcgacga ttatcgacag tccaatgata attgaaccga tgataatgcc gagcgaggtg
     1801 ttctggtcgt cctcgatctc tttcttgatg gagaacgggc agatcagctt gaacaacagg
     1861 aagccgatac ccatgactgc gatgccaacc aatgaataaa taaccgtttc gatgatccct
     1921 attcccacac ttccccaatc catgatggta tggtacctcc gtatttgata ttcttcgtta
     1981 ccgactgccg cgaatcgaat gacacgcaca tttcacgcat gctactttcc cgttcgtatt
     2041 ccgcccggcc tgccccgtcc ataatacccg gcgctcccga cgcgcaccga cttgcgttcc
     2101 gtacgctgtt tgccgatata atagttcccg gcgcgattgt acccgacaaa ctgcgacgac
     2161 atccagtaca ctccgcccag ggcaaggacc actaccacaa tcgcgcgcca catcgcatac
     2221 ctcgcttcac ggtctcaatg gttcgccatt tcgaacggca gttcattgta gttcttcccc
     2281 cgataatgat tcaccaatag cagcacggaa aataatacga tcggaatgaa aaagtaccgg
     2341 aaccctttca cgttttcgag aatcgaaatg gcgattgcgc cggatttccg cggcttttcg
     2401 ctgaacagct ccacatacag ataatagctc ccgggatcat cgaggatgaa atcggtctcg
     2461 cccgaggtat cgctctcgtg ccacttgccc tcgtcgtcgt aaccctcctc gtcccagaag
     2521 tctccgttaa aggcgtatga ttcgacgggt tcctcgaatt catcaatttc cgcaagcgcc
     2581 gttcgtatcc cgtctgcacc cgcctgccgc gactcgaatt gacgaatgcg gtcctccttg
     2641 atcagcagta gacggaacga ttgccattcc aggcgaagcg gttgcacggc gctgtcgacg
     2701 gcgactctga ccgtatacaa tgagtttgaa cgctggaaca ctgcgggacc gaacagtatt
     2761 tggcttgcat acacctgctt cccctgctcg accttcactt cgttttcaac gaggacccgg
     2821 cgggttttcg aaatgtccgg gagcgaatcg ccggaaacgg cggcatatat gatatagatg
     2881 atcgcggcga caatgccgat cccatagata cgcgcacgcc aggcatatgc ggaacgcttc
     2941 ctgcgagttt tttcata
//
LOCUS       DAORHF010000376         2951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_38895_length_2951_cov_3.921961, whole genome shotgun sequence.
ACCESSION   DAORHF010000376 DAORHF010000000
VERSION     DAORHF010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38895_length_2951_cov_3.921961"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="PKJ16_18625"
     CDS             complement(<1..475)
                     /locus_tag="PKJ16_18625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49063.1"
                     /translation="MRLREHGVRLLAFRYNPAGRRPTRKPLGGARQHIETAFLSTGEQ
                     NMQRLISEKLVGLIEKNSASILEKWVERLLADGITSSFSKGHVEYVSDKAKGLLQNLG
                     KWLHYDTNKDEIEKRYTDEAKDLFKMKIPLHEATRAMVVLRRFVWLFVVDETSSDS"
     gene            806..1423
                     /locus_tag="PKJ16_18630"
     CDS             806..1423
                     /locus_tag="PKJ16_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HNV49064.1"
                     /translation="MNIQLKELIDKIKQEGVGEAEAKSRQIIADAEKQAADIVARARK
                     ESDEMVSRAKSQAVQLENDGKEALKQASRDLLLSVKAALTDLLDAIIKNKVSESLSGD
                     VVKEIVPVIVREWVKKGTDDISVLISEKDGEKLASGLMAALAAEMKKGVVIKPHAGIK
                     AGFRIAEKDGSAYYDFTDEGIAENIIQHVNPVLADIIKKSVGGAK"
     gene            1425..1991
                     /locus_tag="PKJ16_18635"
     CDS             1425..1991
                     /locus_tag="PKJ16_18635"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HNV49065.1"
                     /translation="MAQYYYVVASLPALVYDMEKPPALEYFLDACETGVSPEDMAMIR
                     KTHLADVEPEEGESGVLGRWRNWERNLRNKLARLRAAKKSIDAEQFVRENADPHVEDK
                     AAREAFEEASPLAAEDLLDWVRWTYLEELEQGHYFDIDRLVVYYLKLQLLWRKRSFDK
                     ERGAGVFSEIVGKISNEDYSSFKNVVEA"
     gene            2010..>2951
                     /locus_tag="PKJ16_18640"
     CDS             2010..>2951
                     /locus_tag="PKJ16_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002697196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNV49066.1"
                     /translation="MSNIGKVVGVNGNMVRVEFENDVLMNEVAYVKLKEKRLKSEVIR
                     VRGKRAEMQVFEMTKGIGVGDEVEFTSDLLSVSLGPGLLGQIFDGLQNPLPQLAEQCG
                     FFLERGIYLDAIPANRKWKFTPVAKVGDTVTRGEKLGSVPEGIFEHWIMVPFNQYGTY
                     KVKSIAAPGEYAVKDRIAELEDEKGRTISVNMVFQWPVKRAVDVYAERLKPVDPLVTQ
                     IRIIDALFPVARGGTYCIPGPFGAGKTVLQQLTSRYADIDIVIVAACGERAGEVVETL
                     REFPELIDPRTGKSLMERTIIICNTSSMPVAAREASVY"
BASE COUNT          662 a          794 c          902 g          593 t
ORIGIN      
        1 ccgaatccga agatgtctca tcgaccacga acagccatac gaaccggcgc aaaacgacca
       61 ttgcccgggt cgcttcgtga agcgggatct tcattttaaa gaggtccttc gcttcatcgg
      121 tgtagcgctt ttcaatctca tccttgttgg tgtcgtaatg cagccatttc cccagatttt
      181 gcaacagtcc ctttgccttg tcggagacat attcgacatg tcccttggag aatgacgacg
      241 tgatgccgtc ggcaagcaga cgctcgaccc acttctcaag aatcgatgcg ctgttcttct
      301 cgatcagtcc gacgagtttt tcagatatga gtcgttgcat gttctgttcc cccgtagata
      361 aaaatgccgt ttcgatgtgc tgacgtgcgc cgccaagagg ttttcgtgtc ggtcggcgcc
      421 ccgcagggtt gtatcggaat gccagaagcc gcactccgtg ttcccgcagt cgcatgctac
      481 cttcgagcgt aatgatgaaa aatgcaagta aaatatttca cgggaatcgt tttcgctcgc
      541 gcccttcgac gcatgccatt atggcgctgg cacccgcgtc atgagaaata tggaattata
      601 tatacgaaga atgcagtttg taggctttat tttgcgatac atcgcggaaa attttcttga
      661 ttatttcccc agctgcacaa gtattggcct caaagttgaa tatcacgctg ctgcggttct
      721 tttacagtaa ttatttttgt cgatggtgcg tgccggcacg ccggttcaag gtcgcaacca
      781 tatcacgcca cgggaagagg gaaacatgaa catccagctg aaagaactca tagacaagat
      841 aaagcaggag ggcgttggcg aggccgaagc gaagtcccgg cagatcatcg ccgacgcgga
      901 gaagcaggcc gcggatatcg tcgcgcgcgc gaggaaggaa tccgatgaga tggtgtcgcg
      961 cgccaagagc caggcggtcc agctcgagaa tgacgggaag gaagccctca agcaggcgag
     1021 tcgcgatctt ctcctttccg taaaagcggc gctcaccgat ctgctcgatg ctatcataaa
     1081 gaacaaggtg tccgaatccc tctccggcga tgtggtgaag gagatcgtcc cggtgatcgt
     1141 tcgcgagtgg gtcaagaagg gcacggacga catctcggtc ctcatctccg aaaaggacgg
     1201 ggagaagctg gccagcgggc tcatggcggc gctcgctgcc gagatgaaga agggtgtcgt
     1261 catcaagccg catgccggca tcaaggcggg gttccgcatc gcggaaaagg acggcagcgc
     1321 ctactacgac ttcaccgacg aaggaatagc cgaaaacatc atccagcacg ttaatccggt
     1381 gctggccgac atcattaaga aatcggtcgg cggggccaaa tagcatggcg caatactatt
     1441 atgtcgtcgc atcgcttccc gcgctggtgt acgacatgga gaagccgccg gcactcgagt
     1501 attttctcga cgcgtgcgag acgggtgtgt cacccgaaga catggccatg atacgcaaga
     1561 cccatcttgc cgatgtggag cccgaggagg gcgagagcgg cgtgctgggc agatggcgga
     1621 actgggagcg gaaccttagg aacaagctcg ctcggcttcg ggccgcaaag aagagcatcg
     1681 acgcggagca gtttgtgcgg gagaacgcgg atccccacgt cgaggacaag gcggcgcgcg
     1741 aggcgttcga ggaagcatcg ccgctcgcgg ccgaggacct tttggactgg gtgcgctgga
     1801 cgtatctgga ggagctcgag caggggcatt atttcgatat cgacaggctg gtcgtctatt
     1861 atctcaagct ccagctcctg tggcgaaagc gttcattcga caaggagcgc ggcgcgggcg
     1921 tgttttccga aatcgtggga aaaatttcta acgaagatta ttcaagcttc aagaacgtgg
     1981 ttgaagcata gaggcccagg gggaatcgga tgtcaaacat cgggaaggta gtcggcgtaa
     2041 atggcaacat ggtgcgggtc gagttcgaga acgacgttct catgaacgag gtcgcctatg
     2101 tgaagctcaa ggagaagcgc ctcaagtcgg aggtcatccg ggtgcgcgga aagcgcgccg
     2161 agatgcaggt gttcgagatg acgaagggca tcggcgtcgg cgatgaggtc gagttcacct
     2221 cggacctcct gtcggtttcg ctaggacccg gcctcctggg gcagattttt gacggtcttc
     2281 agaatccgct gccgcagctc gccgagcagt gcggcttttt cctagagcgc ggtatctatc
     2341 tcgacgccat cccggccaat cggaaatgga aattcacgcc ggtggccaaa gtgggcgaca
     2401 ccgtcacccg cggcgaaaag ctggggtcgg tccccgaagg aatattcgaa cattggatca
     2461 tggtcccgtt caatcagtat gggacgtata aggtaaaatc aatcgccgcc cccggtgaat
     2521 acgcggtcaa ggaccggatc gccgagctcg aggacgaaaa ggggcgcacc atatcggtga
     2581 acatggtgtt ccagtggccg gtcaagcgcg cggtggacgt gtacgccgag cggctcaaac
     2641 cggtcgatcc cctcgtcacg cagatccgca tcatcgacgc gctcttcccg gtcgcgcgcg
     2701 gcggcacgta ctgcatcccc ggcccgttcg gcgccggaaa aaccgtgctg cagcagctca
     2761 ccagccgcta tgctgacatc gacatcgtga tcgtcgcggc ctgcggcgag cgcgcgggcg
     2821 aggtggtgga aacgctccgt gagttcccgg agctcatcga cccgcggacc ggcaagtcgc
     2881 tcatggagcg gaccatcatc atctgtaata cgagctcgat gccggtcgcc gctcgagagg
     2941 cgtcggtgta c
//
LOCUS       DAORHF010000377         2864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_40818_length_2864_cov_3.554290, whole genome shotgun sequence.
ACCESSION   DAORHF010000377 DAORHF010000000
VERSION     DAORHF010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40818_length_2864_cov_3.554290"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /gene="amrB"
                     /locus_tag="PKJ16_18645"
     CDS             complement(<1..483)
                     /gene="amrB"
                     /locus_tag="PKJ16_18645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HNV49067.1"
                     /translation="MNAKRFVPRICVVLFLCVAFACSVDRAATQTRPSVLAGKWYPSS
                     PAELSREIDGLLDSAARQPSIDNPLVFILPHAGYRYSGAVAAAGYRLMRETAPRPDII
                     VIIAPSHYGDFSGCSTLAVDYFETPLGPVRVDTRIARDLLDNHGFADKPSLHAPEHAV
                     E"
     gene            762..1544
                     /locus_tag="PKJ16_18650"
     CDS             762..1544
                     /locus_tag="PKJ16_18650"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HNV49068.1"
                     /translation="MKIVVCVKEVPDMESKFKIIGDSKIDESQIAFKMNDFDQYAVEA
                     ALQLKEKFGGEVMIVSAGPERVAKEVRQAFAMGADWGIQVQDPEVDAGDSFVVASALF
                     GAIKTIGDVDLVLTGVQAEDDQAAVTGVMIADMMGVPHCTNVVKLDVDGKNMKVNREL
                     EGGKNEVIEMAMPAVLSIQSGMNQPRYPTLPGIMKAKKKRLDVKKAAEVGCTAVGAAG
                     SKTSFVKMFFPVSDHKAEIIQGDPKAAAATLVNKLKNEAKVL"
     gene            1557..2510
                     /locus_tag="PKJ16_18655"
     CDS             1557..2510
                     /locus_tag="PKJ16_18655"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HNV49069.1"
                     /translation="MARILVIAEHRNGALSEGTLELCKAAKAIASAAGAQPAAALLSK
                     DGSLAAELAKYVPEVFTVSDGNLENYTADAYTQAVKAVVDAQDVKGVVIAHSYDGVDF
                     AAKVAMALGCGIVSNCNKIEAQGGNITFTRNTYNGKIQEQKTVKTDKFVATFEKGAFD
                     KEEAGGAGSVTAVSASISASRTKVKEVVETMAGSVDISQATIIVSGGRGTKDKDKYND
                     VIVNLAKKLGGEYAASRPVVDSGWTDAARQVGQSGKTVTPKLYIAAGISGAIQHVAGM
                     KGSQCIVAINKDPEAPIFNIATYGIVGDLFEVIPAMMEAIG"
BASE COUNT          674 a          775 c          838 g          577 t
ORIGIN      
        1 ctcgacggcg tgctcgggcg cgtggagcga gggtttgtcc gcgaaaccgt gattgtcgag
       61 aaggtcgcgc gcgattcgcg tatcaacacg caccgggccc aatggtgttt cgaaataatc
      121 gaccgcgagg gtggagcatc ccgaaaagtc gccataatgc gacggggcga taatgacgat
      181 gatgtccggt cgcggcgccg tttcgcgcat gagacggtac cccgcggccg caacggctcc
      241 ggagtaccga tagcccgcgt gcggcagaat gaaaacgagc gggttgtcga tggacggttg
      301 tcgcgcggcc gaatcgagca gtccgtcgat ctccctcgag agctcggctg gcgacgaggg
      361 ataccatttt ccggcaagga ccgaagggcg cgtctgggtg gcggcccgat cgacgctgca
      421 ggcgaacgca acgcacaaaa aaaggacaac gcaaattcgc gggacaaatc gtttcgcgtt
      481 catcgatcaa tcatacaatt attatggagc atcgcatatc aagagtagcc ctatgggaaa
      541 tcaacagcag cgatcattca atttcaagtt ctttatccgg caatccatga aataattata
      601 caattgttgg ataattgttt cactaatgat ggctttttgt acaggaatta ctcttgacat
      661 caaatacacc catctttaca tggtttataa aagtactttt cattcctttt tctgtacaat
      721 ttaacgtaat ttcgtaaaat tttttaaagg agaggtggat aatgaagatc gttgtctgcg
      781 taaaagaggt tccggatatg gaatccaagt tcaagattat cggcgactcc aagatcgatg
      841 aatcgcaaat cgcatttaaa atgaacgatt ttgaccagta tgccgttgaa gcggccctcc
      901 agctcaagga gaagttcggt ggcgaggtta tgatcgtttc ggccggcccc gagcgggtcg
      961 ccaaggaagt gcgtcaggcg ttcgcgatgg gcgccgattg gggcatccag gttcaagatc
     1021 ccgaggtcga tgccggcgac agctttgtgg tggcctcggc gctcttcggc gcgatcaaga
     1081 ccatcggcga tgtcgatctg gttctcaccg gcgtacaggc cgaagacgat caggccgcgg
     1141 tgacgggcgt gatgatcgcc gacatgatgg gcgttccgca ctgcaccaac gtcgtcaagc
     1201 ttgacgtcga tggcaagaac atgaaggtca accgcgaact cgagggcggg aagaacgaag
     1261 tcatcgaaat ggcgatgccc gccgtccttt ccatccaatc cgggatgaat cagccgcggt
     1321 atcccaccct gcctggcatc atgaaggcga agaagaaacg tctcgatgtg aagaaggccg
     1381 ccgaagtcgg atgcaccgcc gttggcgccg ccggttcgaa gaccagcttt gtgaagatgt
     1441 ttttcccggt ttccgaccac aaggccgaaa tcatccaggg cgacccgaag gccgccgctg
     1501 cgacgcttgt caataagctg aaaaacgaag cgaaagttct gtaagggggg agaaaaatgg
     1561 ctagaatttt agtaattgct gaacacagaa acggtgctct gagcgaagga acccttgagc
     1621 tctgcaaggc cgcgaaggcg atcgcctctg ccgccggcgc ccagcccgca gcggccctcc
     1681 tgtccaagga cggttcgctt gccgccgagc ttgcgaaata tgttcccgag gtcttcaccg
     1741 tgtccgacgg gaacctcgag aattacaccg ccgacgcgta cacccaggcc gtcaaagcgg
     1801 tcgtggatgc gcaggatgtc aaaggcgtgg tcatcgcgca cagctatgac ggcgtcgact
     1861 tcgccgcgaa ggtcgccatg gcgctcggct gcggtatcgt ctccaactgc aacaagatcg
     1921 aagcccaggg cgggaacatc acgttcaccc gcaacactta caacggcaag atccaggagc
     1981 agaagaccgt caaaaccgac aagttcgtcg cgaccttcga aaagggcgcg ttcgataaag
     2041 aagaggcggg cggcgcaggt tccgtaactg ccgtatccgc gtccatcagc gcatcgcgca
     2101 ccaaggtcaa ggaagtcgtc gagaccatgg cgggttcggt cgatatctca caggccacca
     2161 tcatcgtttc cggcggtcgt gggaccaagg acaaggacaa gtacaatgac gttatcgtaa
     2221 acctcgccaa gaagctgggc ggcgaatacg cggcgtcgcg tcccgtggtc gactccggct
     2281 ggaccgatgc ggcccgccag gtcgggcagt ccggcaagac cgtcacgccg aagctgtaca
     2341 tcgcggccgg aatctccggc gccatccagc acgtcgccgg catgaaggga tcgcagtgca
     2401 tcgtggccat caataaggac cccgaagcgc cgatcttcaa catcgccacc tatggcatcg
     2461 tgggcgacct cttcgaggtc attcccgcga tgatggaagc gattggttaa ttagtcggtt
     2521 taacaagcat atgacgcaca aagggacggc atctgccgtc cctttgtgcg ttctggcgcg
     2581 taatcgtcac gtatcggtta aggtcctatt ccagtaggga gtcgaatgag atggccggtt
     2641 gtcacagcga ggtcgctcac ggttatccat gccttcgcga cacgcttcac ctcctgtgaa
     2701 gcgcagcagc ccggattggc tgcctgagcc gcgaaaacat ggatgtattg ttctaggcgg
     2761 ggcgagcgtg acatccggct gtctcatttg gtgttatttg taatgaacct actgaaatag
     2821 gctctaaaac gctcggtgca gcaccccgat cttcaccgag tagt
//
LOCUS       DAORHF010000378         2842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_41296_length_2842_cov_4.780409, whole genome shotgun sequence.
ACCESSION   DAORHF010000378 DAORHF010000000
VERSION     DAORHF010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41296_length_2842_cov_4.780409"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="PKJ16_18660"
     CDS             <1..703
                     /locus_tag="PKJ16_18660"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV49070.1"
                     /translation="HFLPAPRPGLPIGSRYRPVERIGGDFFDFIGQPDPDEIGLFISD
                     VSGHGIPAALYTAMLRVALSQASAHHHDPQGLLERLDRGLGRHVGDAFITCFYGILNI
                     RTGELRYANGGHLPPLVVARDSIQSLPCPPSVPLGMFSRQAAIASSRSFTTEVATLPA
                     GGKLLLFTDGLTEARQPAQQDRLFDEIGLVESIVRARDLPAQDFTDALFADLVRFRGG
                     ESFEDDVCIICVDVA"
     gene            801..1097
                     /locus_tag="PKJ16_18665"
     CDS             801..1097
                     /locus_tag="PKJ16_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49071.1"
                     /translation="MEAEKTGGVVGMLLQAALLVAGIALIVFARYWSRGPAVVASGVF
                     FINLFWITMVLSHDVKLWSVLRNTKAGNVALLAIVLVNLLAIALLALFGDFFKR"
     gene            1255..2019
                     /gene="hisF"
                     /locus_tag="PKJ16_18670"
     CDS             1255..2019
                     /gene="hisF"
                     /locus_tag="PKJ16_18670"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HNV49072.1"
                     /translation="MGTIGIMPCLDMKNGRVVKGVHFVDIKDAGDPVENAKFYQAEGA
                     DELAMLDIAATLENRSTRLEWVRAVASVIGIPLTVGGGIGSLEDIELVLDAGASKVSM
                     NSAAVGNPALVEQAAKKIGPARVTVAIDARRNSAMPSGFELVVSGGTKPIGRDAIEWA
                     RRCQELGAGVILPTSMDGDGTLAGYDLEYTRAIADAVTLPVVASGGAGTLEHFYEGVV
                     KGGASVLLAASVFHFRTFSIRQVKEYLKGRGIEVRL"
     gene            complement(2171..>2842)
                     /locus_tag="PKJ16_18675"
     CDS             complement(2171..>2842)
                     /locus_tag="PKJ16_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HNV49073.1"
                     /translation="KSIAAYERSHELNPFNSRFDDFWRKAVAAGRAVDKIDEKNMAAY
                     TGLGLSDREVRGLMLFNKKGKCAECHVLTTPGAHPPLLTDFTYDNLGTPRNPRNPFYT
                     QERQWNPDGTKWADPGLGGFLKGDPRFARHAAANMGKHKVPTLRNVDLRESASFVKAY
                     LHNGVFTSLKEVVDFYNTRDRDKKRWGPPEVSANVNTEELGDLKLTDDEVGAIVDFMK
                     TLSDR"
BASE COUNT          463 a          860 c          962 g          557 t
ORIGIN      
        1 gcacttcctg ccggcccccc gcccggggct gccgatcggc agccggtatc gcccggtgga
       61 gcgcatcggc ggggattttt tcgatttcat cgggcagccc gatcccgacg agatcgggct
      121 gttcatcagc gatgtctcgg ggcatgggat cccggccgcg ctgtataccg ccatgcttcg
      181 ggtggccctg agccaggcgt cggcgcacca ccatgatccg cagggactgc tggagcgcct
      241 cgaccgcggg ctggggaggc atgttggaga cgccttcatc acctgctttt acgggatcct
      301 gaacatcagg accggggagc tgcgctatgc caatggcggg cacctgccgc cactcgtcgt
      361 ggcccgcgat tcgatacaat ccctgccgtg cccgccgagc gtgccgctcg ggatgttctc
      421 acgacaagcc gcgattgcgt cgtccaggag cttcacgacg gaggtcgcga cgcttcccgc
      481 cggcggcaag ctgctcctgt ttaccgacgg gctcaccgag gcgcgccagc cggcgcaaca
      541 ggaccggctc ttcgacgaaa tcggcctcgt cgaatcgatc gtgcgcgcgc gcgaccttcc
      601 ggcgcaggat ttcaccgacg cgctctttgc ggacctggtg cggtttcgcg gcggcgagtc
      661 gtttgaggac gatgtgtgca tcatttgtgt tgacgtggcc tgatgcgatc acggggcccg
      721 gcgttagctg aagcgcgata gcccatcagt cgtctgtctc ataacgcagt cgcgtcaata
      781 ctctctctgg agggtttcgc atggaagcgg aaaaaacggg aggcgtcgtc ggaatgttgc
      841 tgcaggcggc gctgcttgtc gcgggcatcg cccttatcgt gttcgcccgg tactggagca
      901 ggggccccgc ggtggtcgcc tcgggggtgt tcttcatcaa cctcttctgg ataacgatgg
      961 tcctgtcgca tgacgtcaaa ctctggtccg tgttgcgcaa cacgaaggcg gggaatgtcg
     1021 cgctgttggc catcgtcctg gtcaatctgc tcgcgatcgc gcttctcgcc ctgttcgggg
     1081 atttttttaa aaggtaggat tcgctccgcg tcagggccgt cgcccgatcc gcgaatcggt
     1141 tgacagacgc ggggccattc ggtccgctcc gccgaaaagc ccggtatggc gcggccggat
     1201 tcgatggcgt ataacgcgcg catgcgcgct atcatacacg cacaaggagc agccatggga
     1261 acgataggca ttatgccgtg cctggacatg aagaacggac gcgtggtcaa gggcgtgcac
     1321 ttcgtggata tcaaggacgc gggcgatccg gtcgagaacg cgaaattcta ccaggccgag
     1381 ggggccgacg agctcgccat gctggatatc gccgccacgc tggagaaccg ctccacgcgc
     1441 ctggaatggg tgcgcgctgt cgcctcggtc attggcattc cgcttacggt ggggggcggc
     1501 atcggcagcc tcgaggacat cgagctggtg ctagacgccg gcgcgagcaa ggtgtccatg
     1561 aacagtgcgg ctgtgggcaa tccggcgctc gtggagcagg cggcgaagaa gatcggcccg
     1621 gcgcgcgtca ccgtcgcgat cgacgcgcgg cgtaatagcg cgatgccgtc ggggttcgag
     1681 ctggtggtgt ccggcgggac gaagcccatc ggtagggacg ccatcgaatg ggcgcggcgg
     1741 tgccaggagc tgggcgccgg ggtgatcttg ccgaccagca tggacggcga cgggaccctc
     1801 gcgggatacg atctcgaata cacccgcgcc atcgccgacg cggtgacgct tcccgtagtc
     1861 gcttcgggcg gcgccgggac gctggagcat ttctacgagg gcgtggtgaa gggcggcgcg
     1921 agcgtgcttc tcgcggcctc ggtgtttcac ttcaggacct tcagcatccg ccaggtgaag
     1981 gaatatctca aaggccgcgg gatcgaggta cggctgtagg gcgacgagtg cgcccgaccg
     2041 cgcgccgtgg gttaccggga cgcgcggttc ttcttcgcgt actcccgcag atactggaat
     2101 atcctgttgt tcgaactgtc aggagtgatt ctattccgga atcacccctg acagccctgc
     2161 gcagcgtcgc ttaccggtcc gaaagagttt tcatgaaatc gacgattgcg cccacctcgt
     2221 cgtcggtgag tttgaggtcg ccgagctcct ccgtgttcac gttggccgaa acctcgggcg
     2281 gcccccagcg ctttttgtcg cggtcgcgcg tattgtagaa atcgaccacc tccttgaggg
     2341 aggtgaacac gccattgtga agataggcct tcacgaaact tgccgattcg cgcaggtcca
     2401 cgttgcgaag cgtgggcacc ttgtgcttgc ccatgttcgc ggcggcgtgg cgcgcgaagc
     2461 gcggatcacc cttgagaaag ccgcccaggc ccggatcggc ccactttgtc ccgtcggggt
     2521 tccactgtcg ctcctgcgtg taaaagggat tgcgcggatt gcgcggcgtg ccgaggttgt
     2581 cgtaggtgaa atcggtaaga agcgggggat gcgcacccgg cgtcgtgagg acgtggcact
     2641 cggcgcattt tccctttttg ttaaagagca tgaggccgcg cacctcgcgg tcgctgaggc
     2701 ccagtcccgt gtaggccgcc atgttctttt cgtcgatctt gtcgaccgcg cgcccggcgg
     2761 cgaccgcctt ccgccagaaa tcgtcgaacc gggagttgaa cgggttcaat tcatgcgagc
     2821 gctcgtaggc ggcgatggac tt
//
LOCUS       DAORHF010000379         2833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_41499_length_2833_cov_4.753420, whole genome shotgun sequence.
ACCESSION   DAORHF010000379 DAORHF010000000
VERSION     DAORHF010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41499_length_2833_cov_4.753420"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PKJ16_18680"
     CDS             complement(<1..297)
                     /locus_tag="PKJ16_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HNV49074.1"
                     /translation="MEFLDLFLKTILLRPYVFAFLAAFLFSAILLIGWPRTWRFWLIS
                     WITAFVCEYSSTRNGIPFGWYHYNGSTVGQELYVSNIPFMDSISFSFLLFASYCL"
     gene            complement(387..1370)
                     /locus_tag="PKJ16_18685"
     CDS             complement(387..1370)
                     /locus_tag="PKJ16_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HNV49075.1"
                     /translation="MKALVTGATGFVGGAVARALVKAGVDVRTLSRKGADLQNLAGLP
                     VEQVHGDLRDRESLRQALRGCRQLYHVAAHYALWAKDPSIFYDINVTGTRTLLETARE
                     VGIERTVYCSTIGAIGLPPGGGLGTEETPVSLEQMAGHYKRSKYLAEQEVLKLAREGL
                     PIVIVNPSAPVGEADVKPTPTGQIIVDFMKGRMPAYIETGMNLIDVDDVAQGHLLAMQ
                     KGRQGERYILGNKNLLLNEVFHILSRITGVKAPSVKLPRLAILPLAYANQWISNLTGV
                     PPRIPLEGVKMAKYKMHYDCSKAIRELGLPQTPVDVALEKAVRWFREHGYV"
     gene            complement(1480..2370)
                     /locus_tag="PKJ16_18690"
     CDS             complement(1480..2370)
                     /locus_tag="PKJ16_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HNV49076.1"
                     /translation="MNIKEYLEQKRLAVDHFLDQVSPPAATPPTTLHESMRYSLMAGG
                     KRVRPILTIAAAEALGSTPPGLMAVACSLEFIHTYSLIHDDLPSMDNDDFRRGKPTNH
                     KVYGEAMAILAGDSLLTMAFDLISRPELMKGCDPVRQVRMLQELAYGSGNMGMVGGQV
                     FDIQAENQDIDLPTLQNIHKHKTGMLIRAAVRMGAIAAGATDRQLDDMTGYAEDIGLA
                     FQIADDVLNVTGTREELGKNPNTDAERGKKTYPTFYGVDGARKLADDCVTRAIGRLNT
                     FGPSADPLREIARYITARKN"
BASE COUNT          570 a          788 c          912 g          563 t
ORIGIN      
        1 gaggcagtag ctggcaaaaa gcagaaacga aaacgagatc gagtccatga acggaatgtt
       61 cgaaacgtag agttcctgac cgaccgtgga cccgttgtag tggtaccacc cgaacggaat
      121 gccgttacgc gtcgaggaat actcacagac gaaggcggtg atccagctga tgagccagaa
      181 acgccacgtc cgcggccaac cgatcagcag aatcgctgaa aacaaaaacg ccgcgaggaa
      241 ggcaaagacg tagggacgga ggaggatggt tttgagaaaa aggtcgagaa attccattaa
      301 tgctcaattt tcaatgctca atgggcaatg aaagttgaat ggatttaaaa gtgaaccgtg
      361 agcattgaac attgtgcatc ggatgcctac acatacccat gctcgcgaaa ccaccgcacc
      421 gccttctcca acgcgacgtc aaccggtgtt tggggcaggc cgagttcgcg gatggccttt
      481 gaacagtcgt aatgcatctt gtacttggcc atcttcaccc cttcgagcgg gatgcggggg
      541 ggcacaccgg tcaggttcga gatccactga ttcgcgtagg cgagcggcag gatagccagg
      601 cgcgggagtt tgaccgaggg cgccttcacg cccgtgattc gactgaggat gtgaaagact
      661 tcgttcagta gaagattttt attgcccaga atataacgtt cgccctgtcg gcccttttgc
      721 atcgcgagca ggtggccctg cgcgacgtcg tcgacatcga tcaggttcat gcccgtttcg
      781 atataggcgg gcatccggcc cttcatgaaa tccacgatga tctggccggt gggcgtcggc
      841 tttacgtctg cttctccgac cggtgcgctg gggttcacga tgacgatcgg taggccctcg
      901 cgcgccagct tcagcacttc ctgctcggcg agatatttgg accgtttgta atgtcccgcc
      961 atctgttcca gcgagaccgg tgtctcttcg gtgcccagtc ctccgccggg aggcaagccg
     1021 atcgcgccga tggtactgca atagaccgtg cgctcgatgc cgacctcacg ggcagtttcg
     1081 agcagcgttc tggtgccggt gacattgatg tcgtagaaga tggagggatc tttggcccac
     1141 agcgcgtaat gcgccgccac gtgatagagc tgccggcagc ctcgtagggc ctggcgaagg
     1201 gactcccgat cgcgaagatc gccgtgcacc tgttcgactg gaaggccggc caggttctgc
     1261 agatccgcgc ctttgcggga gagcgtccgg acgtccaccc cggccttgac cagcgcgcgt
     1321 gcgacggctc cgccgacaaa cccggtggct ccggtgacga gcgccttcat ccttgaaccc
     1381 tcgtgaatcg cgacgcgtga cgggtgaagc gttcagggcg caggtaagcc gaccgatgag
     1441 ggatcattcg cttcacgaga tgcgtttcac gaacgacgat tagttttttc gcgccgtaat
     1501 gtagcgcgcg atttcacgga gcgggtcggc tgaggggccg aatgtgttca gccggccgat
     1561 cgcgcgggtc acgcaatcgt ccgccagctt gcgcgccccg tcgacgccat aaaacgtcgg
     1621 ataggtcttc ttcccgcgct cggcatcggt attgggattc ttgcccagtt cttcgcgcgt
     1681 ccccgtgaca ttcagcacat cgtcggcgat ctggaaggcg aggccgatgt cctcggcata
     1741 ccccgtcatg tcgtcgagtt gccgatccgt cgccccggcg gcaatcgcgc ccatgcgcac
     1801 ggcggcgcga atcagcatgc cggtcttatg cttgtggatg ttctggagcg tggggaggtc
     1861 gatgtcctga ttttcggcct gaatgtcgaa gacttgcccg cctaccatgc ccatattgcc
     1921 ggagccgtag gccaactcct gaagcatgcg cacctgccgg acggggtcgc agcctttcat
     1981 gagctcgggc cggctgatga gatcgaaggc catggtcagc agggagtcgc cggccagaat
     2041 cgccatggct tcgccgtaca ccttgtggtt ggtcggtttg ccccggcgaa agtcgtcgtt
     2101 atccatcgag ggcaggtcgt cgtgaatcag ggaataggta tggatgaatt ccagcgaaca
     2161 ggccacggcc atcagcccgg gcggtgtcga gccgagtgcc tcggctgcgg cgatggtgag
     2221 gatcggtcgg acgcgcttgc cgcctgccat caaactgtag cgcatactct catggagggt
     2281 cgtggggggc gtggccgcgg gagggctgac ctgatcgaga aaatggtcca ccgccagccg
     2341 cttctgttcg agatactctt taatgttcat gggtagatac gggacattca cgatgagtgg
     2401 atgggaaccg gcggagagta gcaaaagggt gaaaggggtg tcaatgaaga ggggtgacac
     2461 ccgccagcag ctcctcgctc gcagagcgcg caccaagaac aatcatgagc tgaaatatat
     2521 ggcagtgctc actcgatgcg cgcggtagag ccccttgcag gtgccacccc tacctgggaa
     2581 ggaaacgagc gagcttggag ggaacattgt agctcgcggc tggcgcgccc tcagaaggac
     2641 ctcgcctggg taaatggcgc gtctcgacgc gccagaggtg tgggcgggtg aaacgtatgc
     2701 gcgcaatgga agaccggtgc ggctcccctc gtgagtctgg atgcgagtta gctagaaggg
     2761 gatgtcgcga tgcggcgagc agacggcttc ggtctgacgc agccttgctt cgctgctgta
     2821 gtgggcggga gtc
//
LOCUS       DAORHF010000380         2823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_41757_length_2823_cov_5.226156, whole genome shotgun sequence.
ACCESSION   DAORHF010000380 DAORHF010000000
VERSION     DAORHF010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41757_length_2823_cov_5.226156"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="PKJ16_18695"
     CDS             <1..682
                     /locus_tag="PKJ16_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49077.1"
                     /translation="PDKRVRQIVDALNRDNPVFVVHLGNLVFGGKDWMGFSAKDVRDQ
                     YRHHRNQIKFLRPVLFTVKGELDTFNDSSEIYTAFTRRPRYYSFNNGGIHGIVLDTGD
                     GKAGAIDPNQMKWLEAELARYRDGTGIIVFTHHPLVVPGKNSAPSGVTLSGDGERLHE
                     LFRTYPVKAVFSTSRATALQGHRDGILYCIAACGVTVSTPWQKGAITYYLVDYFNGSC
                     SVYQKRIQ"
     gene            679..984
                     /locus_tag="PKJ16_18700"
     CDS             679..984
                     /locus_tag="PKJ16_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49078.1"
                     /translation="MTAGEPMDAQREITYDGLDGFIDAMKREGVVRLACAEIREKRAT
                     QVEPNVLSVVDLSRLEILAYRRPTIHKCAIDTADFAAVYERLRAQGFEITRRSRNIT"
     gene            990..1949
                     /locus_tag="PKJ16_18705"
     CDS             990..1949
                     /locus_tag="PKJ16_18705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV49079.1"
                     /translation="MDHILQIENLDVSFSLPGARNLAVRGVSVALERGTTLGLVGESG
                     CGKSVTAFSVLRLIQPPGRIDGGSICYNGTDILAMGDEEVRRIRGKEIAMIFQEPMTS
                     LNPVFRVGMQIAETIRLHLGVSKAEARERTVELLRQVGIPSPESRFASYPHELSGGMR
                     QRIMIAMALSAEPQVLIADEPTTALDVTIQAQILDLLLTIQRARRMSLVLITHNLGIV
                     ANLADRIAIMYAGEIVEYGTVADIFASPLHPYTIGLFEAIPKIGAETDRLKTIPGTVP
                     AIAEAPRACVFHPRCFKRGDECLAGPVPLIDKGGERRVRCVRA"
     gene            complement(2092..2334)
                     /locus_tag="PKJ16_18710"
     CDS             complement(2092..2334)
                     /locus_tag="PKJ16_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49080.1"
                     /translation="MKKIVAVLAVCLFVLSFVMVITHTEAGSGFCYMCGSGSPGQYCR
                     AASGKDNSADRNACMKKGCKITGTASCPTAANYKVL"
     gene            complement(2427..>2823)
                     /locus_tag="PKJ16_18715"
     CDS             complement(2427..>2823)
                     /locus_tag="PKJ16_18715"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HNV49081.1"
                     /translation="SLLMLGVAFIYSITSLLGKRGVLCSSPSFFAATYFTLLALPLFA
                     LATARGTLRSVFSRELVAAGLLAAAMISFHMAAIRIAPVSYMIAIKRASLLFGIVFGS
                     LFCGETGLREKLVGGLLMVAGMLGIAMWG"
BASE COUNT          541 a          969 c          860 g          453 t
ORIGIN      
        1 gcccgacaag cgcgtccgcc agatcgtcga cgccctcaac cgcgacaatc ccgtcttcgt
       61 ggtgcacctg ggcaacctcg tcttcggcgg caaggactgg atgggcttta gcgcgaagga
      121 cgtgcgcgac cagtaccgcc atcaccgcaa ccagatcaag ttcctgcgcc cggtgctgtt
      181 cacggtcaag ggcgagctcg acaccttcaa cgactcctcc gagatctaca cggccttcac
      241 gcggcggccg cgctactact ccttcaacaa cggcggcata cacggcatcg tgctggacac
      301 cggcgacggc aaggccggcg cgatcgaccc caaccagatg aaatggctcg aagcggagct
      361 tgcgcgctat cgcgacggca ccggcatcat cgtctttacg caccacccgt tggttgtgcc
      421 ggggaaaaat tcggcgccct cgggcgtcac cctctccggc gacggcgagc gcctgcacga
      481 gctgtttcgc acctatccgg tcaaggcggt cttctcgacc agccgcgcga cggccctcca
      541 ggggcaccgg gacggcatcc tatactgcat cgccgcctgc ggcgtgaccg tatcgacccc
      601 gtggcagaag ggcgccatca cgtattatct tgttgattat ttcaacggct cctgtagcgt
      661 ataccagaag cggatccaat gacggccggg gaaccgatgg acgcccagcg cgagatcacc
      721 tacgacgggc tcgacggctt catcgatgcg atgaagcggg agggcgtcgt ccgcctcgcc
      781 tgcgcggaga tccgcgaaaa gcgcgcgacc caggtggagc cgaacgtgct ctcagtcgtg
      841 gacctgtcgc ggctggagat cctggcctat cgacggccca ccattcacaa gtgcgccatc
      901 gacactgccg atttcgccgc cgtttatgaa cggctccgcg cgcagggctt cgagatcacg
      961 cggcgcagca gaaacatcac ctgatgacca tggaccacat actccagata gaaaacctcg
     1021 acgtatcctt ctcgcttccc ggcgcgcgaa acctggcggt gcgcggcgtg agcgtcgcgc
     1081 tggaacgcgg gaccacgctg ggcctggtgg gcgaatcggg gtgcggcaag tcggtcacgg
     1141 ccttctcggt cctgcgcctc atccagccgc cggggcgcat cgacggcggc agcatctgct
     1201 acaacggaac ggacatcctc gccatgggcg acgaggaggt gcggcgcatt cgcggcaagg
     1261 agatcgcgat gattttccag gagcccatga cctcgctcaa tcccgtcttt cgcgtgggga
     1321 tgcagatcgc cgaaaccatc cggctccatc tgggcgtctc gaaggccgag gcgcgcgagc
     1381 ggaccgtcga gctcctccgc caggtgggca tcccgagccc ggagagccgc ttcgcgagct
     1441 acccgcacga gctctccggg ggcatgcgcc agcgcatcat gatcgcgatg gccctctcgg
     1501 ccgagccgca ggtgctcatc gccgacgagc cgaccaccgc gctcgacgtg accatccagg
     1561 cgcaaatcct ggacctcctg ctcaccatcc agcgcgcgcg ccgcatgtcg ctggtcctca
     1621 tcacccacaa cctggggatc gtggcgaacc tcgcggaccg gatcgccatc atgtacgccg
     1681 gcgagatcgt cgagtacggc accgtcgccg acatcttcgc gtcgccgctg catccgtaca
     1741 ccatcggcct cttcgaggcc atcccgaaaa tcggcgccga aaccgaccgg ctcaagacca
     1801 tccccggcac cgtccccgcc atcgccgagg ccccacgcgc gtgcgtgttc catccgcgct
     1861 gtttcaagcg cggcgacgaa tgccttgcgg ggccggttcc gctgatcgac aagggcgggg
     1921 agcgtcgggt gcggtgcgtg cgggcgtagc gtaagctttc gaagggccgc cgtgcggcgc
     1981 gatcccccaa acggccgcac atgccgcatg gacgcgccaa taaacagacg agcggcgcga
     2041 gtgtttgacc cgcgccgctc gtgtggcact acaatcgtaa tgcgttcgct gctacagcac
     2101 cttgtagttc gcagcggtgg ggcaggaagc ggttccggtg atcttgcatc ccttcttcat
     2161 gcaggcattc cggtccgcgc tgttgtcctt cccagaggcc gctctgcaat actggccggg
     2221 gcttcccgaa ccgcacatgt agcagaatcc tgatccggct tcagtatggg taatcaccat
     2281 gacaaacgaa aggacgaaca gacatacggc gagaacggcg acgatcttct tcatagagcc
     2341 tccctgttaa atagttaccc aaagcatact ccccgggcgg tccgtcgtca acacaaattt
     2401 cggaccctgc ggggaccggg gacgcatcaa ccccacatgg cgataccaag catcccggcc
     2461 accattaaca atccccctac cagcttctcc cgcaggcccg tctcgccaca aaacagcgat
     2521 ccgaacacga tgccaaagag caggctggcg cgcttgatcg cgatcatgta gctcaccggg
     2581 gcgatccgga tcgcggccat gtggaagctg atcatcgccg ccgcgagcag ccccgcggca
     2641 acgagttcgc gcgagaacac ggaccgcagc gtcccgcgcg cggtcgcgag cgcgaacagc
     2701 ggcagcgcca ggagcgtgaa gtaggtggcg gcgaagaacg acgggctcga gcacagcacc
     2761 ccgcgcttcc ccaggagcga ggtgatgctg tagatgaagg cgactcccag catgaggagc
     2821 gac
//
LOCUS       DAORHF010000381         2814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_41946_length_2814_cov_4.611091, whole genome shotgun sequence.
ACCESSION   DAORHF010000381 DAORHF010000000
VERSION     DAORHF010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41946_length_2814_cov_4.611091"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="PKJ16_18720"
     CDS             <1..1294
                     /locus_tag="PKJ16_18720"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HNV49082.1"
                     /translation="CVVGMANTACTSVRSGAVFSMPGVMDTITNIGITQEIIDHFAAR
                     DPWFAYDCYRRLIQDFAISTYGIDRSVFENLMQRAKDEAGVPLKEKLTGAQMEALTRK
                     YRYVINKHSYSIPKDPYTQLFYAIIAVYRSWESTIAKNYRRFVNLSDAWGTAVIVQKM
                     VFGNYSPTDITGVVHSAYLGRENISLFGEYKTRAQGHDIVSGVAKVFPISEEQKWAYP
                     RLRSFVSLEKEFPETYRLLFDTVRRIRQRWGNDVEIEFTLENGVLYILQIRGMVKHAF
                     ETEELVGHPDELQDYLLGQGLAASGGAVSGRVVFDIDRIDLVRGKYAGDKIILVRPET
                     NPEDVIGLEKSDGILTCIGGMTSHAVLQMRRLEKSGVSDFSIMRIDEENRQGIITNED
                     PEEGITIIREGDFITIDGATGHVYLGYHRTRKKTYVVR"
     gene            1442..2593
                     /locus_tag="PKJ16_18725"
     CDS             1442..2593
                     /locus_tag="PKJ16_18725"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HNV49083.1"
                     /translation="MSTISHTTPIGVNGIGRIGKLMAWALTHRAEQESIAISTGREVG
                     AGIKDLAAYLEYDSTYGNYAKFYWGANPKKRVEIRDKTLYLNGVKVIWLNDPKYRVAG
                     EIPWADHGVELVFDTTGKATDPTITEPKGLRGHLAHSKKVVLTAPFKIKNKGALMPDD
                     AVTVVGGVNIHAYDPDKHAIISNASCTTNCCAPPIKALVDHFGDKLLSFAITTVHAAT
                     NSQSTLDTVPTAGSKDTRKNRSTFNNMIPTSTGAANAVLEVIPEIRTLGIGSQASSIR
                     VPTNTGSIVVMDVSIMGEYDDAYMHGIMRSYSEKYPEVMRYSDESLVSADIIGSTHST
                     VYDAPFTHRRTSKRGSEYFTMFDLNFWYDNEYGYVCSLLRLYDHMTGRG"
BASE COUNT          571 a          941 c          869 g          433 t
ORIGIN      
        1 ctgcgtggtc ggtatggcga acacggcctg cacgtcggtg cgcagcggcg ccgttttctc
       61 catgccgggg gtgatggaca ccatcacgaa catcggcatc acccaggaga tcatcgacca
      121 cttcgccgcg cgcgatccct ggttcgccta cgactgctac cgccgcctga tccaggattt
      181 cgcgatctcc acctacggga tcgaccgctc cgtcttcgag aacctgatgc agcgcgcgaa
      241 ggacgaggcc ggcgtcccgc tcaaagagaa gctcaccggc gcgcagatgg aggcgctcac
      301 gcgcaagtac cgctatgtca tcaacaagca ctcgtactcc attcccaagg acccgtacac
      361 gcagctcttc tacgccatca tcgcggtgta ccgctcgtgg gagtcgacga tcgcgaagaa
      421 ctaccggcgg ttcgtgaacc tctccgacgc gtgggggacc gccgtgatcg tgcagaagat
      481 ggtcttcggc aactactcgc ccacggacat caccggcgtg gtgcacagcg cctacctggg
      541 gcgcgagaac atcagcctct tcggcgaata caagacgcgc gcccaggggc acgacatcgt
      601 cagcggcgtc gcgaaggtct tccccatcag cgaggagcag aagtgggcct acccgcgcct
      661 gcgctcgttc gtctcgctcg agaaggagtt ccccgagacc taccggctcc tcttcgacac
      721 ggtgcggcgc atccggcagc gctggggcaa cgacgtggag atcgagttca ccctcgagaa
      781 cggcgtgctc tacatcctgc agatccgcgg gatggtgaag cacgccttcg agaccgagga
      841 gctggtgggc catcccgacg agctgcagga ctacctgctg ggacagggcc tggcggcctc
      901 cggcggcgcg gtgtcggggc gcgtggtctt cgacatcgac cgcatcgacc tggtgcgcgg
      961 caagtacgcg ggcgacaaga tcatcctggt gcgccccgag accaatccgg aggacgtgat
     1021 cggcctggag aaatccgacg gcatcctcac ctgcatcggc gggatgacct cgcacgcggt
     1081 gctgcagatg cgccggctcg agaaatccgg cgtgagcgat ttttccatca tgcgcatcga
     1141 cgaggagaac cggcagggaa tcatcacgaa cgaggacccc gaggagggca tcacgatcat
     1201 ccgcgagggg gattttatca ccatcgacgg cgcgacgggg cacgtgtatc tggggtatca
     1261 ccggacgcgg aagaagacgt atgtggtgcg atgaccccct ccgtccctcg gacacctccc
     1321 ccatcgacag ggagaggaca gacgcgatcg cgagagcacg accgctcatt gtcgacacgc
     1381 gggcggcgtt tctcatagac tacggcagga aagcaccaaa ccatcaccca aggaggaacc
     1441 aatgagcacc atatcgcaca caacgccaat cggcgtgaac ggcatcgggc gtatcggcaa
     1501 gcttatggcg tgggcgctca cgcaccgcgc agagcaggag tcgatcgcga tctccaccgg
     1561 tcgcgaggtg ggcgccggga tcaaggatct cgccgcctat ctggaatacg attcgaccta
     1621 cgggaattac gcgaaattct actggggcgc gaacccgaag aagcgggtcg aaatccgcga
     1681 caagaccttg tacctgaacg gcgtcaaggt gatctggctc aacgacccga agtaccgcgt
     1741 cgcaggcgag atcccctggg cggaccacgg cgtcgagctc gtcttcgaca ccaccggcaa
     1801 ggcgaccgat ccgaccatca ccgagcccaa gggccttcgc ggccacctcg cgcacagcaa
     1861 gaaggtggtc ctcacggccc cgttcaagat caagaacaag ggggcgctga tgcccgacga
     1921 cgccgtgacc gtcgtgggcg gcgtgaacat ccacgcctac gatcccgaca agcacgccat
     1981 catatccaat gcgtcgtgca ccaccaactg ctgcgcgccg cccatcaagg cgctggtgga
     2041 ccacttcggc gacaagctgc tctcgttcgc catcaccacc gtgcacgccg cgacgaactc
     2101 gcagagcacg ctggacaccg tccccacggc cggctccaag gacacgcgca agaaccgcag
     2161 caccttcaat aacatgatcc ccacctccac cggcgccgcc aacgcggtcc tcgaggtgat
     2221 cccggagata cgcacgctgg gcatcggctc gcaggcctcg tccatccgcg tccccaccaa
     2281 caccggctcc atcgtggtca tggacgtctc catcatgggc gagtacgacg acgcctacat
     2341 gcacgggatc atgcggagct attcagagaa atatccggag gtgatgcgct actccgacga
     2401 gagcctggtg agcgcggaca tcatcggcag cacccactcc accgtgtacg acgcgccctt
     2461 tacgcaccgg cgcacctcga agcgcggcag cgagtatttc acgatgttcg atctcaactt
     2521 ctggtacgac aacgagtacg gctatgtgtg ctcgctcctg cggctctacg accacatgac
     2581 cggcagggga tgattcgaca gggcgtgcgc gcatcgggaa agtcgttcgg gaacagggtg
     2641 ataccggtgc gcggcgcgcc gcgcaccgcg tcccatgcac ggaacgtcgg gcgcacccgg
     2701 cccgcggccg ggtcccggcg cgaacggacc gttaggcgcg cggcgtgccc atgaggaagg
     2761 tgtcgatgtc gaccgccgcg cgctttccgg cgcccatggc gctgatgacg gtcg
//
LOCUS       DAORHF010000382         2796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_42354_length_2796_cov_3.424298, whole genome shotgun sequence.
ACCESSION   DAORHF010000382 DAORHF010000000
VERSION     DAORHF010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42354_length_2796_cov_3.424298"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PKJ16_18730"
     CDS             complement(<1..237)
                     /locus_tag="PKJ16_18730"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HNV49084.1"
                     /translation="MNEFSVVLFHSTNQAIWASKVFAQAGLEYSMIPVPRHLSSDCGY
                     CLRIPSSLADAAAALMRDRGVEFERIAALDGQATS"
     gene            complement(275..718)
                     /locus_tag="PKJ16_18735"
     CDS             complement(275..718)
                     /locus_tag="PKJ16_18735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49085.1"
                     /translation="MKRKRMPYVGWAIVIGMAFALLSAGCASVLFQDLHRETRVFSVP
                     LAESDKAWSRVQYYVYTVMGYTNTIQSNSGTIRVTDTAFSRDYNDLTIIRVVKNEKPV
                     FTVQFKKMLYGDDDEVKDQVGKMKGRLAELEEYAKTGVLPSVPKY"
     gene            865..2082
                     /locus_tag="PKJ16_18740"
     CDS             865..2082
                     /locus_tag="PKJ16_18740"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HNV49086.1"
                     /translation="MNPFKKSAQHQNTFLVSIGAGFNQLPLIDEARKQRYHVIGVDRD
                     SRAAGFIKCDIKIQESLENHHDIYLKLRELLIDGQIKAVLSRSFGIAVRTACYIANQL
                     RIPMMPFERVDQCINKRAMKEAFARNGIVSPQYRIITPKTAAARLRKEPFPAIIKPVV
                     GHAKTNVRRVNDAAALSEFLASAPPDHEWILEEFIDGEEIIAVGIVHKKRFHLVEVSD
                     KVKTNPPHFVDIMHVAPSRHLDRRREISEIGQRLAEAFDMYTTPLVMELIAVPGARLH
                     LIEAVPEFGGEYLADYLIPTHTGYNLFGETIRAISDTGFTPPQFDRARGAVAVRYITG
                     TPGTLASFNPENPKGHRDTVYSRIFREVGAQIKSPTSNHDRIGVVITKGKTADEAVAA
                     ACRAEESFNLRII"
     gene            2093..2740
                     /locus_tag="PKJ16_18745"
     CDS             2093..2740
                     /locus_tag="PKJ16_18745"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HNV49087.1"
                     /translation="MTRDIKPWEQHYGRDRSRLGYPDEALVRMLRPWIAERNCTALRA
                     LDLGCGSGRHLSLLAESGVMEPVGLDLSAAALAQCRELGFTRLLRSDNSNIALRSASF
                     DIVVAWGSLHYCRKDETARMIGEIRRIIRPGGALFGTLRSDRDSYLRRGEHIGDNTWI
                     TDLDDIRQSVASFFGESEIVSLFGGFARFRYGIMERSPLGDTSRILSHWFYTAEA"
BASE COUNT          555 a          915 c          828 g          498 t
ORIGIN      
        1 ggaagtagcc tgcccgtcga gcgccgcgat gcgctcgaac tcgacgccgc gatcccgcat
       61 gagcgcggcg gccgcatcgg ccagcgagct ggggatgcgc aggcaatacc cgcagtccga
      121 gctcaggtgg cgcggcacgg ggatcatcga atactccaac ccggcctgcg caaacacctt
      181 tgacgcccag atcgcctggt tggtggagtg gaacaggaca acggaaaact cattcatgat
      241 cgcgctcgcg gcgttgcgtc gggcacgcgc cgcatcagta cttcggcacg ctgggcaaca
      301 cgccggtttt cgcatactct tccaattcgg caaggcggcc cttcattttg ccgacctggt
      361 ccttgacctc gtcatcgtcc ccgtagagca tttttttgaa ctgaaccgta aaaaccggct
      421 tttcgttctt aaccacccgg atgatggtga gatcgttgta atcccgtgaa aacgcggtgt
      481 cggtgacccg tatggtgccg gaattggact ggatggtgtt ggtgtacccc atcaccgtat
      541 agacatagta ctggacccgc gaccatgcct tatcggactc cgcgagcgga acggaaaaaa
      601 cgcgggtttc ccggtgcagg tcctggaaca ggacgctcgc acaacctgcc gataaaaggg
      661 cgaaggccat gccgataact atcgcccaac cgacgtatgg catacgtttt cttttcatga
      721 attccgattg tccgggcctc gggaaatagg caagcacatt ttcccgcctg cccgaaggac
      781 ccgaagccga tttttcattg tgttttttac cggcggcggc cacagtgtgc cgattaatag
      841 ctgaggcggt ccatatcggc accaatgaac ccgtttaaaa agagcgcaca acaccagaac
      901 accttcctcg tctccatcgg ggccggcttc aaccagttgc cgctgatcga cgaggcgcgc
      961 aagcagcgct atcatgtcat cggggtcgat cgcgactcgc gcgccgcggg cttcatcaaa
     1021 tgcgacatca agatccagga atcgctggag aaccatcacg acatctacct caagctccgc
     1081 gagctgctca tcgacggcca gatcaaggcg gtgctgtcgc gctcgttcgg gatcgccgtg
     1141 cgaacggcct gctacatcgc caatcagctc cgcatcccga tgatgccctt cgagcgcgtg
     1201 gaccagtgca tcaacaagcg cgccatgaag gaggccttcg ccagaaacgg catcgtgtcg
     1261 ccgcagtatc ggatcatcac ccccaaaacg gccgccgcgc gcctccgcaa agaaccgttc
     1321 cccgcaatta ttaagccggt ggtgggacac gccaagacga atgtccgccg ggtgaacgac
     1381 gccgccgctc tctcggaatt cctcgcgtcg gccccgccgg accacgagtg gatactcgag
     1441 gagttcatcg acggggagga gatcatcgcc gtgggcatcg tccacaagaa gcgcttccac
     1501 ctggtggagg tgagcgacaa ggtgaagacc aacccgcccc atttcgtgga catcatgcac
     1561 gtggcgccgt cgcgccatct cgaccgcaga cgcgagatca gcgagatcgg ccagcgcctt
     1621 gccgaggcgt tcgacatgta caccacgccg ctggtgatgg agctcatcgc cgtgcccggc
     1681 gcgcgcctgc acctcatcga ggcggtgccg gaattcggcg gcgagtacct cgccgattac
     1741 ctcatcccga cgcacacggg ctacaacctc ttcggcgaga ccattcgcgc catcagcgat
     1801 accggtttta ccccgccgca gttcgatcgc gcccgcggcg cggtcgccgt gcgctacatc
     1861 accgggacgc ccggcacgct cgcctcgttc aacccggaaa accccaaggg ccatcgggac
     1921 accgtctact cgcgcatctt cagggaggtg ggcgcccaga tcaaatcccc gaccagcaac
     1981 catgaccgca tcggcgtggt catcacgaaa ggaaaaacgg ccgatgaagc ggtcgcggcg
     2041 gcatgccggg ccgaagaaag cttcaatctg cgcatcatct gaacacgcga acatgacacg
     2101 cgacatcaaa ccatgggaac agcactacgg gcgggaccgc tcccgcctgg gctatcccga
     2161 tgaagcgctg gtccggatgc tgcgcccctg gatcgccgag cggaactgca ccgcgctccg
     2221 cgcgctcgac ctcggctgcg gcagcggtcg ccacctctcc ctcctcgcgg aatcgggcgt
     2281 catggaaccg gtggggctcg acctgagcgc cgcggccctc gcgcaatgcc gggagttggg
     2341 cttcacccgg ctcctccgct ccgacaattc gaacatcgcc cttcgttccg cgtcgttcga
     2401 catcgtggtt gcgtggggtt cgctgcacta ctgccgcaag gacgagacgg cgcgcatgat
     2461 cggcgagatt agacggatca tacggccggg aggcgcgctc tttggcaccc tgcggagcga
     2521 tcgggacagc tacctgcgcc gcggcgagca tatcggcgac aatacctgga tcacggacct
     2581 ggacgacatc aggcaatcgg tcgcatcttt tttcggggag tcggaaatcg tgtcactgtt
     2641 cggcggcttt gcccgctttc ggtacggcat catggagcga tcgcctctgg gcgacacgtc
     2701 gcgaatccta tcacactggt tctacacggc cgaggcatga gcggcgcatc ggcggccttc
     2761 gagacgcggc gggacgattg cccgctctgt ggcgca
//
LOCUS       DAORHF010000383         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_42989_length_2770_cov_4.190792, whole genome shotgun sequence.
ACCESSION   DAORHF010000383 DAORHF010000000
VERSION     DAORHF010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42989_length_2770_cov_4.190792"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PKJ16_18750"
     CDS             complement(<1..1389)
                     /locus_tag="PKJ16_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HNV49088.1"
                     /translation="MIREKKVIAAEREYEAVKEKDIPISHYFGEDTFNSRVQQMKLPQ
                     SMYKRLQAIINEGSQLDIETANAVAHAMKEWAIEKGATHYSHWFQPMTGATAEKHDSF
                     IEMVSADEVIEKFSGKQLVQGEPDASSFPSGGLRTTFEARGYTAWDISSPAFILRSGI
                     GTTLCIPTVFISYSGEALDKKTPLMRSNRAVNESALRLMKLLGDTGAKRVFSTLGPEQ
                     EYFLIDKDYYFKRQDLVLANRVLTGAAPPKGQQLEDQYFGSVKERILSFMHDVEEELY
                     KLGIPAKTRHNEVAPSQFEIAPIFEEANLAADHNQLVMEMLRKVAKKHNLAALLHEKP
                     FAGINGSGKHLNWSLSDDRGNNLLNPGKNPHENLQFLVILVATIRAVYLHADILRASV
                     ASSGNDHRLGANEAPPAIISVFLGEQLTKILEDIEKGAAAKATDEAIIDLGISSLPAV
                     SKDNTDRNRTSPF"
     gene            1736..>2770
                     /locus_tag="PKJ16_18755"
     CDS             1736..>2770
                     /locus_tag="PKJ16_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49089.1"
                     /translation="MKRIKIIAVFAAAALLFSCSSAGKKDGPKEDKVKEGEGKMYWKD
                     GSLRGVGQYKDYKKNGKWTLYYQMTGEKQGEGDYKDDEQHGPWIYFHKKGPKMAEGSF
                     DAGQKTGEWVEFYETGQMFTKAAYVVSDVTVEGITSRVGGIEGIKKTFFLSGKVKSEE
                     EYKKGLKNGITREYYEDGKPKEFSQYRDNEYDGKVNKFHPNGRRSEESFYAAGKRQGK
                     YDAYYDNGPASITGQFEQNKQSGMWKFFARDNRVMKEGLYKEGREEGYWAFYEYVGGK
                     SKFMDATITGGMITGNCIVYKNGKKLGEGQLKQSIPKPQCVEKCDDCTECKTWSGKWI
                     MPAKNGQWTEY"
BASE COUNT          628 a          772 c          821 g          549 t
ORIGIN      
        1 gaacggcgag gtccggttgc ggtcggtgtt gtccttgctc accgccggca gcgacgagat
       61 gccgaggtcg atgatggcct cgtcggtcgc cttggcggcg gcgcccttct cgatgtcctc
      121 caggattttc gtgagctgct cgcccaggaa caccgagatg attgcgggcg gcgcctcgtt
      181 tgcgcccaaa cggtgatcgt tgcccgacga ggccaccgat gcgcgcagga tgtccgcatg
      241 cagatagacc gcgcggatcg tcgcgacaag gatcaccaga aactgcaggt tctcgtgcgg
      301 gtttttgccg ggattgagca ggttgttccc gcgatcgtcg gagagcgacc agttgaggtg
      361 cttgccggag ccgttgatac ccgcgaacgg cttttcgtgc aagagcgccg cgaggttgtg
      421 cttcttggcg accttgcgca gcatctccat gaccagctga ttatggtccg ccgccaggtt
      481 cgcctcctcg aagatgggcg cgatctcgaa ctggctcggc gcgacctcgt tatggcgcgt
      541 cttcgccggg atgcccagct tatagagctc ctcttcgaca tcgtgcataa aggaaaggat
      601 gcgctccttc acgctcccga agtactggtc ttcgagctgc tgccccttgg gcggcgcggc
      661 gccggtaagc acgcgattgg ccagcaccag gtcctgccgc ttgaagtagt agtccttgtc
      721 gatgagaaag tattcctgct cgggtcccag cgtcgagaag acccgcttcg cgccggtatc
      781 gcccagcagc ttcatgagcc ggagcgcgct ctcgttcacc gcacggttgg agcgcatgag
      841 cggggtcttt ttatcgagcg cctcgccgga atacgatatg aataccgtgg ggatgcacag
      901 cgtggtgcca atgccgctgc gcaggatgaa cgcgggactc gagatgtccc acgcggtgta
      961 cccgcgcgcc tcgaaggtgg tgcgcaatcc accgcttggg aagcttgacg cgtcgggctc
     1021 gccctgcacg agctgtttgc cggaaaattt ctcgatcacc tcatcggccg aaaccatctc
     1081 gatgaacgaa tcgtgcttct cggcagtcgc gccggtcatg ggctggaacc agtggctgta
     1141 atgggtcgcc cccttttcga tcgcccattc cttcatggcg tgggcgaccg cgttcgcggt
     1201 ctcgatatcc aactggctgc cttcgttgat gatcgcctgc aaacgcttgt acatgctctg
     1261 cggcagcttc atctgctgca cccgcgaatt aaaggtatcc tcaccgaagt agtgggatat
     1321 cgggatgtcc ttttccttga ccgcctcgta ctcgcgctcg gcggcaataa cttttttctc
     1381 ccgtatcata atgcctccac aaaaagattc tcgttatcgt gttgaatgca acatagttcg
     1441 caccccggtt tttttcaatt aacaaacccg gatttcatat tacgtacttg taaactttat
     1501 ggaacatatg tgtcgtatcg gaatcgtgcg ccccgcgcat cccctgtgca tggtgcacat
     1561 gcagcggtca aacacaataa aaatgacgat tatcgggcgc aatccgggcc ttaaaaagtt
     1621 acacgcgcgt taaattttat acttgaagta taactgcgcc taaaataggc tatcgttggt
     1681 gcgagggacg caccgcccat acaccagcca tatcaaccaa gtaaggagta accatatgaa
     1741 acgcatcaag ataatcgcgg tgttcgctgc agccgcactg ctgttttcct gctcctcggc
     1801 cgggaagaag gacggcccca aggaagacaa ggtcaaagag ggcgaaggaa agatgtactg
     1861 gaaggacggc tctctgcggg gcgtgggaca atacaaggac tacaagaaaa acggcaaatg
     1921 gaccctctac taccagatga ccggcgaaaa gcagggcgag ggcgactata aagacgatga
     1981 acagcacggc ccatggatct actttcacaa aaaaggaccc aagatggccg agggctcctt
     2041 tgacgccggc cagaagactg gcgagtgggt cgagttttac gagaccgggc agatgttcac
     2101 caaggccgcg tatgtggtca gcgacgtgac ggtggagggc atcacctccc gggtcggcgg
     2161 aatcgagggc atcaagaaaa cctttttcct ctccggcaag gtcaagagcg aagaggagta
     2221 caagaagggc ctcaagaacg ggatcacccg ggaatactac gaggacggca agccgaagga
     2281 attttcgcag tatcgcgata atgaatacga cggcaaggtg aacaagttcc atcccaacgg
     2341 gcgccgctcg gaggaatcgt tctacgccgc cggcaagcgc cagggcaagt acgacgccta
     2401 ctacgacaat ggccccgcca gcatcaccgg acaattcgaa cagaacaagc aaagcggcat
     2461 gtggaagttt ttcgcccgcg acaatcgggt catgaaggaa ggcctgtaca aggagggccg
     2521 ggaggaaggc tactgggcct tctacgaata tgtcggcggg aaatcgaagt tcatggacgc
     2581 caccatcacc ggcgggatga tcaccggaaa ctgcatcgtc tacaaaaacg gcaagaagct
     2641 cggcgagggg cagctcaagc aatcgatacc caagccgcaa tgcgtggaga agtgcgacga
     2701 ctgcaccgag tgcaagacct ggtcgggcaa gtggattatg ccggcgaaga acggccagtg
     2761 gacggaatac
//
LOCUS       DAORHF010000384         2735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_43781_length_2735_cov_6.225000, whole genome shotgun sequence.
ACCESSION   DAORHF010000384 DAORHF010000000
VERSION     DAORHF010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43781_length_2735_cov_6.225000"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="PKJ16_18760"
     CDS             <1..512
                     /locus_tag="PKJ16_18760"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HNV49090.1"
                     /translation="SGALPGDCICVTGTLGKNEAGLRLLMAGKPGYTQGFLEPRARRA
                     DEGRVIARYAHAMIDVSDGLASEVRHICEQSGAGAIIECDAIPISAETRDAAAAVGSD
                     PSAYALYGGEDFELVFTAERGAIDTLRSVFQDFTIVGEILSPDGGIRLRKGERLVELD
                     QGFDHFAKT"
     gene            complement(608..811)
                     /locus_tag="PKJ16_18765"
     CDS             complement(608..811)
                     /locus_tag="PKJ16_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014957708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HNV49091.1"
                     /translation="MTKGTVKWFDEKKGYGFLSQENGPDVFVHHSGIAGEGFKVLYEG
                     DRVEFEIEKSDKGPRAVQVRTIQ"
     gene            complement(1002..1409)
                     /locus_tag="PKJ16_18770"
     CDS             complement(1002..1409)
                     /locus_tag="PKJ16_18770"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HNV49092.1"
                     /translation="MPFIVWTEDLSVGVKAFDEDHKQLVGYVNKLHLGMQAGERIDSM
                     SHILDGLIDYTITHFKREQDLMAANSYPEYEDHKMQHEILTNRVLEFKELIKNGKSAF
                     TIELMVFLRDWLTGHIKGSDMRYREFFTKKGIS"
     gene            1674..2480
                     /locus_tag="PKJ16_18775"
     CDS             1674..2480
                     /locus_tag="PKJ16_18775"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV49093.1"
                     /translation="MTFAIAPFPQRFLVPMMAACIMLSANPVAIGAPSPADDHNRQGL
                     ALYKSGKLEEAAALFHRAITADPNHKWGHYNLACVYARLFECEQIQLGERMLDEREIY
                     RHLEKAISLDRTFIERAKKDRDFERVYGKVGYLTTTGLSVKDPAHVRTILCSVRWYLN
                     ECSGGVYGCSTMTFGKDGTVRYNHYDPCEYAEGNDARDAERAKAECARLKKMHHGTFR
                     IENSKIVVRFNNGETLRYAFPSRDGRIRETGGSKTRDWNLRDSDPNACSA"
     gene            2544..>2735
                     /locus_tag="PKJ16_18780"
     CDS             2544..>2735
                     /locus_tag="PKJ16_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV49094.1"
                     /translation="MNVTFWGTRGSLPAPIDASTVKTKIERVLADVRDGRFTAAELLE
                     GGLAANYGHGAYGTYGGNTT"
BASE COUNT          611 a          796 c          781 g          547 t
ORIGIN      
        1 gctcgggcgc cctcccgggc gactgcatct gcgtgaccgg gaccctcgga aaaaacgagg
       61 cgggcctccg ccttctgatg gcgggaaaac ctggatacac gcagggcttt ctcgagccgc
      121 gcgcacggcg ggccgatgag gggcgcgtca tcgcccggta cgcgcatgcc atgatcgacg
      181 tgagcgacgg gctggcaagc gaggtgcggc acatctgcga acagagcggc gccggcgcga
      241 tcatcgagtg cgacgcgata ccgatatccg ccgagacgcg ggatgccgcg gccgcggtgg
      301 gctctgaccc ctctgcgtac gctctctacg gcggcgagga tttcgagctg gtgttcacgg
      361 ccgagcgggg cgctatcgac acacttcgta gcgtattcca ggattttacc atagttggcg
      421 agatactatc ccccgacggg gggatccggc tccggaaggg cgagcggctt gttgagcttg
      481 accagggctt cgatcatttc gcaaaaacat aagtctcgat tggctggatg ctcgcctgga
      541 gcacgatttc aataaaaaaa gcccatccga ttggatgggc tttctggttg tccgtttttc
      601 aaatggctta ctgaatggta cggacttgaa cggcgcgggg gcccttgtcg cttttctcaa
      661 tttcgaactc aacacgatcg ccttcataga ggaccttgaa gccttcgccc gcaataccgg
      721 aatgatgtac gaacacatcc ggaccgtttt cctgagagag gaaaccgtaa ccctttttct
      781 cgtcaaacca tttaactgtt cctttcgtca tgctgcctgt actccctgta cgtatttaaa
      841 aaatgccggt tataccggag gaaccgcctc gaacatcaat tatgtttacc ttgagaaaag
      901 cggaaagata atcggatagt cactgaagct gttcttaagc taaccatggt gcaaaggcgg
      961 agggacgtgt caacgaaatt tactgaaaat aataaaaaat attacgaaat tccctttttt
     1021 gtgaagaact cccgatagcg catatcgctg cccttgatat gcccggtgag ccagtcccga
     1081 aggaacacca tgagctcgat ggtaaaggcg cttttcccgt ttttgatcaa ttccttgaac
     1141 tccagcaccc ggttggtgag gatctcatgc tgcatcttgt ggtcctcata ctcgggatag
     1201 ctattggcgg ccatgaggtc ctgctcccgc ttgaaatgag taatcgtata gtcgatgagc
     1261 ccgtcgagga tgtgcgacat gctgtcgatc cgctcgccgg cctgcatgcc caggtgcagc
     1321 ttgttcacat acccgaccag ctgtttatgg tcctcatcga aggccttcac gccaacgctc
     1381 aagtcttcgg tccacacgat aaacggcatc tcccccctcc ccggcacatg cgccaattag
     1441 ggcaagtata gaaagcgctc ccggtgtttt caagtttttt cagggaccgc gtcgaaaaac
     1501 atcgacggtg agcgtctgta ccgccggtcc actcgcacgg gatggccgtt gccggacgga
     1561 aaccggcgcc tcatcggatt atactgttga caggacgggt tttcacagta ggctgcgctg
     1621 atgggagaac gaattcttac atccgcacca gccgcccgga ggaaccccgc cccatgacat
     1681 tcgccattgc gccctttccg caaagattcc ttgtgccgat gatggcggca tgcatcatgc
     1741 tctccgccaa tcccgtcgca atcggcgcgc catcgcccgc cgacgaccac aatcgccagg
     1801 gcctggcgct gtataaaagc ggaaaactcg aggaggccgc tgcgctgttc caccgggcga
     1861 tcacggccga tcccaaccac aaatggggcc actacaacct cgcctgcgtc tatgcccgac
     1921 tgttcgaatg cgaacagatc cagctcgggg aacgcatgct ggacgaaagg gaaatctatc
     1981 ggcatctcga aaaggccatt tcgctggacc gaacgtttat cgagcgcgcc aaaaaggaca
     2041 gggacttcga gcgcgtttac ggcaaggtcg gctatctgac gacgacgggc ctcagcgtaa
     2101 aggatccggc gcatgtccgc acgatcctgt gctcggtgcg ctggtatctc aacgagtgca
     2161 gcggaggcgt gtacggatgc agcacgatga ccttcgggaa ggacggcacg gtgagatata
     2221 accattacga cccctgcgaa tacgccgagg gaaatgatgc ccgtgacgcg gaacgggcca
     2281 aagcggaatg cgctcgcctc aagaagatgc accacggcac atttcgcatc gagaattcga
     2341 aaatcgtcgt tcgattcaac aacggcgaga cgctgcgcta tgcgttcccg tcgcgtgatg
     2401 gccgaattcg ggaaaccggc ggctcaaaaa cgagggactg gaacctgcgc gacagcgatc
     2461 ctaatgcgtg cagcgcgtaa cgacacccgc gcagctcggc gcgactgctg acgctatcaa
     2521 tcaatacgag cggagccggt acgatgaatg taaccttctg gggcacgcgg ggatcgcttc
     2581 ccgcgccgat cgatgcaagc actgttaaga ccaagatcga gcgcgtcctc gcggacgtgc
     2641 gcgacggccg cttcacggcc gcggaactgc tcgaaggcgg tctcgcggcg aactacggac
     2701 acggcgcata cgggacctac ggcggcaaca cgacc
//
LOCUS       DAORHF010000385         2731 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_43886_length_2731_cov_5.270179, whole genome shotgun sequence.
ACCESSION   DAORHF010000385 DAORHF010000000
VERSION     DAORHF010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43886_length_2731_cov_5.270179"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..495)
                     /gene="rnpB"
                     /locus_tag="PKJ16_18785"
     ncRNA           complement(149..495)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PKJ16_18785"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(578..1147)
                     /locus_tag="PKJ16_18790"
     CDS             complement(578..1147)
                     /locus_tag="PKJ16_18790"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HNV49095.1"
                     /translation="MIAGSLKGRAIPFDNRRYGGAETSPQRLKGALFSMLGEDLSGKA
                     FLDLYACSGQMGIEAYSRNARPVVMNERDGRRYEHLIGLVGAWGLADGCLTMRMDAAQ
                     CVRHCAERGMRFECVFIDPPYAKNAGEASVYGEILGFLDEAAVLAPGGRIVVQHFARN
                     TLPDNVGRLRMIDSRTYGTSALSSYLPDR"
     gene            complement(1166..1360)
                     /locus_tag="PKJ16_18795"
     CDS             complement(1166..1360)
                     /locus_tag="PKJ16_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HNV49096.1"
                     /translation="MKIKETTTVEEAIKKGPRVVEVFQQYRLDCPSCRGLKEDTIRIV
                     AVNNGLDLKKLLDDLNAAMS"
     gene            1469..1657
                     /gene="rpmB"
                     /locus_tag="PKJ16_18800"
     CDS             1469..1657
                     /gene="rpmB"
                     /locus_tag="PKJ16_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HNV49097.1"
                     /translation="MAKCQVCGKSVQFGNNVSHSNKKTSKVWHANIKRIRIVDNGSVK
                     RTYVCTRCIRTGKVTKAS"
     gene            complement(1759..2613)
                     /locus_tag="PKJ16_18805"
     CDS             complement(1759..2613)
                     /locus_tag="PKJ16_18805"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV49098.1"
                     /translation="MERSVFDWNGSRVVLHRAGSGSPILFLHNGGNDHRIWDYQVARF
                     SRSHAVIAVDLPGYGDSDRPRTSYTLSFYEGFVRDCIDHLSLERPVLVGNCVGSAMAV
                     RYALSCPDRVAGLVLCNVLTERTLSAGVFGPLYRAIVRHPMVRRLVARWTGNAAVRRI
                     AREISPSMQYGESGDPDPAFIGHLRARYAHPEHLRVLYDLLCNIPDFAQLDRMAKPAG
                     FPKTCVIWGEQNRVLPWSAGREWCERFVPERMVVLPKSGHLIMRELYDQVNECIDGFL
                     SELQAAQG"
BASE COUNT          561 a          873 c          775 g          522 t
ORIGIN      
        1 cggatcgcct cgtcggttca tttccaaagg cgtcccgcaa tgtctcgtgg cggacatggg
       61 cggggccccg gcgcccggca agcatacccc gacgggggcg aataaatcaa acatttttag
      121 cgcgacggcg aagcgatgcg ggaaaaacta tccgggggga tcataagccg aattctgttt
      181 atgcagccat ttatcttgga cggctgttgc cagccgcctc tttgcagcct acccgcaacc
      241 tcggacgggc cgtcctcgat cggttgctta tttggccttg caccggacgg ggtttgcact
      301 gccgccctcc tcgcggagga cgcggtgagc tcttacctcg ccttttcacc cttaccccgt
      361 tgggggcggt atgttttctg ttgcactttc catcgccttg cggcgcccgg gcgttacccg
      421 gcgtcctgcc ctacggtgtt cggactttcc tcaccgcctg catgcgcagg caagcgcggc
      481 tgcttgatcc ccccggatag ggaatcatat ctataaaacc attcgcgttc gctgaacgca
      541 tgaaactgca gtcaatcaca tcgaacaaat accgtaatca tcggtccggc aggtagctcg
      601 aaagggcgct ggtcccgtat gtccgcgaat cgatcatccg caaacgcccc acattatcgg
      661 gaagcgtgtt tcgcgcaaag tgctggacaa cgatccgtcc ccctggagca agcacggcgg
      721 cctcgtctaa aaaaccgaga atctcgccgt acacggacgc ctcaccggcg tttttcgcgt
      781 agggagggtc gataaacacg cactcgaatc gcatcccccg ctcggcgcaa tgacggacgc
      841 actgtgcggc gtccatgcgc atcgtgaggc agccgtccgc gagaccccat gcccccacca
      901 gcccgatgag gtgctcgtac cgccgtccgt cccgttcgtt catgacgacc ggtcgggcgt
      961 tgcggctgta tgcctcgatg cccatctggc cggagcaggc gtacaaatcg agaaaggcct
     1021 tccccgaaag gtcctcgccc agcatggaaa agagcgcccc tttcagtcgc tgcggcgagg
     1081 tctcggcccc gccgtatcgc cgattgtcaa atggaatggc gcggcccttc aggctgccgg
     1141 cgatcacccg catcgtcgaa cacggtcatg acatcgcggc attgaggtcg tccagcagtt
     1201 ttttcaggtc gagcccgttg ttcaccgcca cgatgcgtat cgtgtcctcc ttgagcccgc
     1261 gacaggaggg gcaatccagc cggtactgct gaaacacctc aacaacccgc gggcccttct
     1321 ttatcgcctc ttcgaccgtt gtcgtttctt tgatcttcat tgagcctcgg tttcaatatg
     1381 cgcaaaaatc ggtccatgac gaaaaaatct tgcatttttt tagcatgact tctaagagtg
     1441 tctatcaatt tttcgtacgg ggtacatcat ggccaaatgc caagtttgcg gtaaatcggt
     1501 tcaattcgga aacaacgtga gccactccaa caagaaaacg tcgaaggtgt ggcacgccaa
     1561 catcaaacgg atacgcatcg tcgacaacgg aagcgtcaag cggacgtatg tgtgcacgcg
     1621 ctgcatccgc acgggaaagg tcaccaaggc cagctgacga gcatctccgc ccaccggcgg
     1681 tcaacgcgaa ccgatgccgg accgtactgg ttcggcattc gcgttttacc gccatgtgtc
     1741 catcctcgcg aggcgccatc acccctgcgc cgcttgtagt tcgcttagaa acccgtcgat
     1801 acactcgtta acctgatcgt acaactcgcg catgatcagg tgcccgcttt tcggaaggac
     1861 gaccattcgc tccgggacga accgttcgca ccactcacgc cccgcggacc atgggagcac
     1921 acggttttgc tcgccccaga tcacgcacgt cttcggaaaa ccggccggtt tcgccattcg
     1981 atcgagctgg gcaaagtccg gaatattgca caggaggtcg tacagcacgc gcaggtgctc
     2041 gggatgcgca tagcgggcgc gcaggtggcc gatgaacgcc gggtcgggat cacccgactc
     2101 cccatactgc atcgacggtg aaatctcgcg ggcgatgcgc cgaacggcgg cattcccggt
     2161 ccatcgcgca acgagccgcc gtaccatggg atgacggacg attgcccggt aaagcggccc
     2221 aaagacgccg gcgctcaggg tgcgctcggt gagcacattg cacagcacca gtcccgcgac
     2281 acggtcggga cacgaaagcg catagcgcac ggccatggcg ctccccacgc agttcccgac
     2341 cagaacggga cgctcgagag agaggtgatc gatgcagtca cgaacgaaac cttcgtagaa
     2401 cgacagcgtg taggatgtgc gcggtcggtc ggaatcgcca tagccgggca ggtccaccgc
     2461 aatgaccgca tgcgaacggg agaagcgggc gacctgataa tcccaaatcc ggtgatcgtt
     2521 cccgccgttg tgaaggaaaa ggatgggcga tccgctcccg gcgcggtgca ggacgacccg
     2581 gcttccgttc cagtcgaata cgcttcgctc catggcctga tccccatcgc gcgccgtctc
     2641 cgatggcgac ggaccggcgg tcaccttacc catttaccaa cgccaccatg agcatgccga
     2701 tgagcaccat cgcgaagaac agggcgagcc c
//
LOCUS       DAORHF010000386         2698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_44709_length_2698_cov_3.482028, whole genome shotgun sequence.
ACCESSION   DAORHF010000386 DAORHF010000000
VERSION     DAORHF010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44709_length_2698_cov_3.482028"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..872)
                     /locus_tag="PKJ16_18810"
     CDS             complement(159..872)
                     /locus_tag="PKJ16_18810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49099.1"
                     /translation="MAYIEGKMMAENAGAGTKRLAILSLSGALVVLLVFLLPATRWTV
                     FSTVTLAVVLCLIIAYMYFRMDPYAWKRIFDRPGYERLRELDRRVIESLRLLDDSCLV
                     LVNTTVELFHIEYLVVSQKGIYLLSKIEGTGMTGCENGIPRVGEHSLGNEVALLWRTC
                     HFLGILMKKTYQVDVLPHPVLVSSDTGAFIMDNCAEITITSCESIPSIITRASGPPLP
                     EGAAENFAYFIKTRYMSAG"
     gene            complement(1125..2561)
                     /locus_tag="PKJ16_18815"
     CDS             complement(1125..2561)
                     /locus_tag="PKJ16_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49100.1"
                     /translation="MSIKRDFGSVQPGIPLGPLTLRVPFIHYRFEWADFAQGLLMCTV
                     CLSIITVLTAKLGMSFEVALAIVVLNGALYLAHVTFGDPVVPGWVTPAIPLLIAYCET
                     FPIGAERIQALIAFEMTFGVFCIFLGITGLAKRFIALIPNAVQSAILIGAGFAAIMLI
                     FDPAKQGIRSFSTSPLTISVCVGLAFYFIFSESFKAFRRKHRWAYYLGNLGMMPALAL
                     AVILGPLFGELPWPTYDGSVFTSPDFATLWKDFTILGAIPFPPLDMFISSVPMVISAY
                     IVVFGDVIQSQALLDDAQKYRPDENVNYNPNRSHIIFGGRNLVMSIAGPDISMCGPIW
                     AAMQVVVCERFKQGPKAMESINSGAGSFRWGTFTGYFFAPIVSTVKPILGVGLASTML
                     IQGYVSVRVGVLKSRAFNDLGIAGVAGAILAVRGASWGLSAALILTALVYISKNWKKA
                     YEQEEPVFPCDSPECIAREVQETMGNAH"
BASE COUNT          580 a          750 c          778 g          590 t
ORIGIN      
        1 taatatcgat ctgctcgccg ggctgatcaa gaagaacgta aagctggaag agatctgata
       61 catcattccc gggggcgcgc cgtggcgtgt ccccagtttc cgtattcctt tctcctcatg
      121 cccgggcgct ttcacccccc atcgcgtccg gcggcatttc atccggcgga catatatctg
      181 gtctttatga aatacgcgaa gttttcggcc gctccttccg gaagcggcgg cccggaagca
      241 cgcgtgatga tcgatggaat gctctcgcag gatgttatgg tgatttccgc gcagttgtcc
      301 atgatgaagg ctccggtgtc ggacgaaacc agtaccgggt gcggcaatac atcaacctgg
      361 tatgtttttt tcatcaggat gcccaggaag tggcaggtgc gccagagcag tgccacctca
      421 tttcccaggg aatgctctcc aacgcgggga attccgtttt cgcaccccgt cattcccgtc
      481 ccttctattt tcgacagcag gtatattccc ttttgggaaa ccaccaggta ctctatgtgg
      541 aacaactcga ccgtggtgtt gaccagcacc aggcatgagt cgtcgagaag acggagcgat
      601 tcaattacgc gccggtcgag ctcgcgcagg cgttcatagc ccggtctgtc gaagatccgc
      661 ttccacgcgt acggatccat cctgaaatac atgtaggcga taatgaggca caaaacgacc
      721 gcgagcgtga cggttgaaaa gaccgtccac cttgtcgccg ggagcagaaa gacgagcagc
      781 accaccagcg cgccggaaag ggagagtatg gcaagccgtt tcgtgccggc gccggcgttt
      841 tcggccatca ttttaccttc aatatatgcc attggccgcc ttctccgttc tcgtttttgg
      901 tattcagttc ctggcgtcgt gtttcgtccg cagatcgcgg attcgtagtc ttcccgtccc
      961 gcccgataat gacatgtgtt tttattgtcg tccaccaata aaagaaacga gtgccgcagc
     1021 gcaggtattc gcggcactcg ttgatcaggt aagggtccgg acgggatcgt ttgcctgagg
     1081 ccgggagcgg cgcggcccca tgctgtctcc ccccggttga tcgatcaatg cgcgtttccc
     1141 atggtctcct gaacttccct ggctatgcat tcgggggaat cgcaggggaa caccggttct
     1201 tcctgttcgt atgccttctt ccagttcttg ctgatgtata cgagcgcggt gagtatcagg
     1261 gcggccgaga gtccccacga ggcgccgcgg accgcgagaa tcgcacccgc gacgcccgcg
     1321 atgcccaggt cgttgaaggc ccgtgacttg agcacgccga cgcgcacgct gacgtaaccc
     1381 tggatcagca tggtggatgc gagccccacg ccgagtatcg gcttgaccgt cgagacgatg
     1441 ggggcgaaga aataaccggt gaacgttccc cagcgaaacg agcctgcgcc actattgatc
     1501 gattccatag cctttgggcc ctgcttaaaa cgctcgcaca ccacaacctg catggctgcc
     1561 caaattgggc cgcacatcga tatatcgggt ccggctatgc tcattactag gttccggccg
     1621 ccgaagatta tgtgcgatct gttggggttg tagttaacat tttcgtcggg acggtatttc
     1681 tgggcgtcgt cgagaagggc ttggctctgg atgacgtctc caaataccac gatgtacgca
     1741 cttattacca tcgggacact cgagatgaac atatcgagcg gcgggaaggg aatggcaccg
     1801 agaatcgtga aatccttcca gagggtggca aaatccgggg aggtgaaaac gcttccgtca
     1861 taggtcggcc aagggagctc gccaaaaagc ggtcccagta tgacggcaag cgcgagtgcc
     1921 ggcatcatcc cgagattgcc aagataataa gcccatctgt gtttgcggcg gaaagccttg
     1981 aagctttcgg aaaaaatgaa gtagaaggcg agacccacgc agacgctgat ggtcagcgga
     2041 ctagtgctga agctccgtat gccctgcttt gcgggatcga agatgagcat tatggctgcg
     2101 aagccggcgc cgataagaat ggccgactgc acggcgttgg gaatcagcgc gatgaagcgc
     2161 ttggcgagcc ccgtaatccc aaggaagatg cagaacacgc cgaacgtcat ttcgaacgct
     2221 atcagcgcct gtatgcgctc ggctccgatc gggaaggtct cgcagtaggc gatcaggagc
     2281 ggaatggcgg gagtaaccca gcccggtaca accgggtcgc cgaaggttac atgcgccagg
     2341 tacagggccc cgttgagaac gacgatggcc agtgcgacct cgaaagacat gccgagcttg
     2401 gcggtgagca ccgttatgat cgaaaggcat acggtgcaca tcagaagccc ctgggcgaaa
     2461 tcggcccatt cgaatcggta atgaataaac ggcactctca gtgtcagcgg gcccaggggt
     2521 ataccgggct ggaccgatcc gaaatctctc ttgatactca tactatagtc acctcctatc
     2581 tatttatcat gtctttcagg tcgtttgggt gagaccgccc ccccggacat ttcaatgcgt
     2641 gccgctccgt tctttgacgg tttttcgtct tattgtgtaa acctctcgtg gaagcgtt
//
LOCUS       DAORHF010000387         2690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_44914_length_2690_cov_3.654269, whole genome shotgun sequence.
ACCESSION   DAORHF010000387 DAORHF010000000
VERSION     DAORHF010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44914_length_2690_cov_3.654269"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PKJ16_18820"
     CDS             <1..283
                     /locus_tag="PKJ16_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49101.1"
                     /translation="STMTPWGWFRHRWITVKWIICCYGVIVGTYPLGPWLAEMVAIAD
                     RAGLGAFADPVYRHNRTMLMILGTVQTGTLVFACFVSALKPWRNRRAAA"
     gene            395..2563
                     /locus_tag="PKJ16_18825"
     CDS             395..2563
                     /locus_tag="PKJ16_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HNV49102.1"
                     /translation="MRRRLLLLAIAILPLASCAKPVSIDLSKDWQAHHIDITKADLYG
                     AGLPWSATDERCRTDALEASAWSPVPSLPAALTLERKKQLCWLRKEVVVPESFRGKNL
                     SLYLGKIWDAQTTYLNGVRIGVSGSEYPNFHSDWNVAVSHFLPPELIRYGEKNVIAVR
                     QFSDQQLNFNGEPFIGDDHRVRVYAFWMRFMAEYLVMGLGVMTMLVGAGMLMYFFLSG
                     RRDRVSLHFGSISVLWFVLTAHFWAPGFGALSWRLHDNLFYVLVGALVVWIYVSLETI
                     LKYRFRWARIVVGVIFAAQTVMALTSTVEDPVTGWRFNLMAPMGLAVQLLWGFVLVRG
                     IIDKKPEAKILAIGYFIFFATLVHDGLMMNRVIMSYAFMSNIAYPGFILSFAIIIFRR
                     IALMNEELRRSTMEIGEKNERLEQLMKSVIESTDELIGIAVTVKESSATLSEQMESQT
                     ASLEETSASIEEVSGSIASIADHAALQDELVKRSEGTLAGHIRSLGEITEAATYAVSL
                     GTRSREQTGSITERLGRVTKGMMKIKESSASIEVIADMINEIAEKTNLLSLNAAIEAA
                     RAGEYGRGFAVVADEIGKLADSSVEQAKTIQNIIHGVVGNIDEETRIIMESSQAVTAL
                     NAAAEDVNGAVSGIMRLCEAQNAQTASIRDNMQSISMGSTEISTATKQEQIAMHEVLT
                     TILSLNEVVDRVNDSTARMVEISEKLAHRIALLNRIVVEQ"
BASE COUNT          504 a          845 c          875 g          466 t
ORIGIN      
        1 ttcgacgatg acgccgtggg ggtggttccg ccatcgctgg atcaccgtga aatggattat
       61 ctgctgttac ggcgtgatcg tcgggaccta tccgctgggc ccctggctcg ccgagatggt
      121 cgccatcgcc gaccgcgcgg gtttgggagc cttcgcggac ccggtgtacc ggcacaatcg
      181 gaccatgctg atgatcctgg gcacggtgca gaccggcacg ctcgttttcg cctgtttcgt
      241 gtcggcgctc aagccgtgga ggaaccggcg cgccgccgcg tgacggccgg gaacggccgc
      301 accgggcgag gaaaatgacc ggcaccggga aaatgtggtg tacagaagcg gcgtcggcga
      361 tcaggatgtc cccataatga ttggggttgc ctcaatgcgc agacgattgc tgctcctcgc
      421 catcgcgatc ctccccctcg cctcctgcgc caaacctgtt tccatagacc tgtcgaagga
      481 ctggcaggcg catcacatcg acatcacgaa ggccgatctc tacggcgcag ggcttccctg
      541 gagcgcgacg gacgagcgct gccgcaccga cgcgctcgag gcatccgcgt ggtcgccggt
      601 cccgtcgctc ccggcggcgc tcaccctgga gcgcaaaaaa cagctctgct ggctgcggaa
      661 ggaggtggtg gtccccgaat ccttccgcgg aaagaacctc tcgctgtatc tgggcaagat
      721 ctgggacgcc cagacgacct atctcaacgg cgtgcgcatt ggcgtttccg ggagcgagta
      781 tccgaatttc cattccgatt ggaacgtggc ggtgagccac ttccttcctc ctgagctcat
      841 ccgctacggc gagaagaacg tgatcgccgt gcggcagttc agcgaccagc agctcaactt
      901 caacggcgag cccttcatcg gcgacgacca tcgcgtgcgc gtgtacgcct tctggatgcg
      961 cttcatggcg gaatacctgg tgatggggct gggcgtgatg accatgctgg tgggcgcggg
     1021 catgctcatg tacttcttcc tcagcgggcg gcgcgaccgg gtgagcctgc acttcggttc
     1081 aatctcggtc ctgtggttcg tgctcaccgc gcatttctgg gcgccgggat tcggcgcgct
     1141 ctcctggcgg ctgcacgaca acctgttcta tgttctggtg ggcgccctgg tcgtgtggat
     1201 ctatgtgagc ctggagacca tactcaagta ccgcttccgc tgggcgcgga tcgtcgtggg
     1261 cgtcatcttc gccgcacaga cggtcatggc gctcacgagc accgtggagg accccgtcac
     1321 cggctggcgc ttcaatctca tggcgccgat ggggctcgcg gtgcagctcc tgtggggttt
     1381 tgtgctggtg cgcggcatca tcgacaaaaa gcccgaggcg aagatcctcg ccatcggtta
     1441 tttcatcttc ttcgcgacgc tggttcacga cgggctcatg atgaaccgcg tgatcatgag
     1501 ctacgccttc atgagcaata tcgcctatcc cgggttcatt ttgtccttcg ccatcatcat
     1561 cttccggcgc atcgcgctga tgaacgaaga gctccggcga agcaccatgg agatcgggga
     1621 aaagaacgag cggctcgagc agctcatgaa gagcgtgatc gagtccaccg acgaactcat
     1681 cgggatcgcc gtcaccgtga aggagtcctc cgcgaccctg agcgagcaga tggagtcgca
     1741 gacggcgagc ctggaggaga cctccgcctc catcgaggag gtgagcgggt ccatcgcctc
     1801 catcgccgac cacgccgcgc tgcaggacga gctcgtgaag cgcagcgagg ggacgctcgc
     1861 gggccacatc cgctcgctgg gcgaaatcac cgaggcggcg acctacgccg tgtcactggg
     1921 gacgcgcagc cgcgagcaga ccggctccat caccgagcgc ctgggccgcg tgacgaaggg
     1981 gatgatgaag atcaaggagt cgtcggcctc catcgaggtg atcgccgaca tgatcaacga
     2041 gatcgccgag aagacgaacc tcctgtccct gaacgcggcc atcgaggcgg cgcgcgccgg
     2101 cgagtacggc cgcggcttcg cggtggtcgc cgacgagatc ggcaagctcg cggacagctc
     2161 ggtggagcag gccaagacta tccagaacat catccacggc gtggtgggca acatcgacga
     2221 ggagacgcgc atcatcatgg aatcgtcgca ggccgtgacg gcgctcaacg cggccgcgga
     2281 ggacgtgaac ggcgccgtga gcgggatcat gcggctctgc gaggcgcaga acgcgcagac
     2341 cgcgagcatc cgggacaaca tgcagagcat ctcgatgggg tccacggaga tatccaccgc
     2401 caccaagcag gagcagatcg cgatgcacga ggtgctcacc accatcctgt cgctcaacga
     2461 ggtggtggac cgcgtgaacg acagcaccgc gcggatggtg gagatatccg agaagctcgc
     2521 gcaccgcatc gcgctcctca accgcatcgt ggtggagcag taaggattga caaccgcggg
     2581 gcgcgcggct agggtgccgc ggtacgcgcc ccaatcacac caaaacggga aaacggcaat
     2641 gaacgcgata ttcaactaca ccggcaacgg cgcctgtccc ctgtgccgca
//
LOCUS       DAORHF010000388         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_45124_length_2682_cov_3.703464, whole genome shotgun sequence.
ACCESSION   DAORHF010000388 DAORHF010000000
VERSION     DAORHF010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45124_length_2682_cov_3.703464"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="PKJ16_18830"
     CDS             <1..206
                     /locus_tag="PKJ16_18830"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49103.1"
                     /translation="GKYQLAAPFRCEAVGWNMADAMERHANNGPIDLVYRLSLNEYNG
                     RVTSQLVLIDLRESGSSNEKINI"
     gene            273..572
                     /locus_tag="PKJ16_18835"
     CDS             273..572
                     /locus_tag="PKJ16_18835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV49104.1"
                     /translation="MDIKINDGNTQVDIVVSGDIEMMTIKEFKEKLFEIGQNANKDVE
                     IDLSKVDYIDSSGVGVLISLLKMQKKKGKALKIEKVSSQVLNVLQLSSLSDVFNL"
     gene            634..1122
                     /gene="ispF"
                     /locus_tag="PKJ16_18840"
     CDS             634..1122
                     /gene="ispF"
                     /locus_tag="PKJ16_18840"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HNV49105.1"
                     /translation="MDSFRIGIGYDVHRLVQGRPCIIGGVRIPFDRGLDGHSDADVLV
                     HAIIDALLGAAGLGDIGAHFPDTDERYRGADSLRLLTAVHEMLVARSVAIVNIDSVVQ
                     CQMPKILPHAEEMKRAIGRALGDLDISRIGIKGKTTEGLGFPGRGEGIAAQAVALIMI
                     RA"
     gene            1241..2455
                     /gene="tyrS"
                     /locus_tag="PKJ16_18845"
     CDS             1241..2455
                     /gene="tyrS"
                     /locus_tag="PKJ16_18845"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013011551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HNV49106.1"
                     /translation="MIHNDVELLKRGVEEIIPEDEFVKKVERSQRENRPLIVKAGFDP
                     TAPDIHLGHTVLLRKMRHFQSMGHTIVFLIGDFTGMIGDPSGKQDTRKRLTREEVLAN
                     AETYKKQVFKILDREKTVVDFNSRWCEQMRFADVLELTAKYNVARMLERDDFANRYRD
                     GKPISILEFLYPLIQGYDSVALKADIELGGTDQKFNLLVGRDMQREYGQEPQVIMTMP
                     LLVGLDGVQKMSKSLGNYIGINEPPKEIFGKVMSISDDLMFWYYELVTDVPLDEVAGL
                     KAGIADGSVHPRDVKVRLGKEICAQFYDRETADAAEAEFNRIFVRKDLPDTIEEYAIP
                     ESEKSDGKIWIVKLLVLSGMAKSNGEARRLISGGGVYIDSDRVEKDDYELPLPATCVI
                     KVGKRRFMKITG"
     gene            2487..>2682
                     /locus_tag="PKJ16_18850"
     CDS             2487..>2682
                     /locus_tag="PKJ16_18850"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HNV49107.1"
                     /translation="MSDYSDDAVIARVLAGDADAFEEIIKRYNDKLFRYVYSKVNNYD
                     EAADVTQDIFVMAYESLASFR"
BASE COUNT          631 a          715 c          787 g          549 t
ORIGIN      
        1 acggcaaata ccagctggcg gccccattcc gctgtgaggc ggtgggctgg aacatggccg
       61 atgccatgga acgccatgcc aacaacggcc cgattgacct ggtgtatcgg ctgtcactga
      121 acgaatataa cgggcgtgtc acttctcaat tggtcctaat cgacctgcgc gaaagcggat
      181 catcaaacga aaaaattaat atttgacttt tatcgccatt gatgaaattg taacgccatt
      241 gaaactcacg gggcgggaat tgggtagcca ctatggatat aaagataaat gacggaaaca
      301 cacaggttga tattgtcgtc agcggcgata ttgaaatgat gacgatcaaa gagttcaagg
      361 aaaagctctt tgaaattggc cagaatgcca acaaagatgt cgaaatcgac ctttccaaag
      421 ttgactacat cgattcatcg ggtgttggcg tcctcattag ccttctgaag atgcaaaaaa
      481 agaagggaaa agcacttaaa atagaaaaag tcagctcgca ggtcctcaat gttcttcagc
      541 tcagttcgct atcggacgta ttcaatctgt gacggaatcg gtgcgctccg ttttcaatag
      601 cgcgcgctcg ttgcatccat aacggattat cccatggatt catttcgcat cggcatcggg
      661 tacgacgtgc atcgattggt gcaggggcgc ccgtgcatca tcggaggagt gcgcataccg
      721 ttcgaccgcg ggctcgatgg ccactccgac gccgatgtcc tggttcacgc gatcattgat
      781 gcgcttctcg gggccgccgg ccttggcgat atcggcgcgc actttccgga caccgacgag
      841 cgctatcgtg gcgccgacag ccttcgcctg cttacggccg tccatgaaat gcttgtggcc
      901 cggtcggttg cgatcgtcaa catcgattcg gtcgtgcagt gccagatgcc gaagatcctt
      961 ccgcacgcgg aggagatgaa gcgggcgatc ggacgcgcgc tgggcgatct cgatatttcc
     1021 cgcatcggta tcaaggggaa aacgacggaa ggcctcgggt ttcccggacg gggagagggg
     1081 atcgccgcgc aagcggtcgc gctcatcatg attcgcgcgt gatggcgtaa aaaatgttat
     1141 tgcagaaacc cgccatctga tttgtggtgc aatctccgga cgataccgga cgcgcatgcg
     1201 actcgattat gcgcgcacgc ggcactacag ggattgtgcg atgatacaca atgacgtgga
     1261 actactgaaa cgcggcgtcg aggagattat ccccgaggac gaattcgtga aaaaggtcga
     1321 gcggtctcag cgcgagaacc ggccgctcat cgtgaaggcg ggattcgatc ccaccgcccc
     1381 cgacatccac ctgggccata cggtcctcct gcgcaagatg cggcacttcc agtccatggg
     1441 acacaccatc gtgttcctga tcggcgactt caccggtatg atcggcgatc cctcgggcaa
     1501 gcaggacacc cgcaagcgcc tgacacgcga ggaggtgctg gccaatgcgg aaacgtacaa
     1561 aaaacaggta ttcaagatcc tcgatcggga gaagacggtc gtcgatttca actcgcgatg
     1621 gtgcgagcag atgcgcttcg ccgacgtgct cgagctcacc gcgaaataca atgtcgcgcg
     1681 catgctcgaa cgtgacgatt tcgccaaccg atatcgggac ggcaagccca tatcgattct
     1741 ggaattcctc tatccgctga tccagggcta cgactcggtc gcgctcaagg ctgacatcga
     1801 actgggcggc acggatcaga aattcaacct gctcgttggc cgggacatgc agcgcgagta
     1861 cggccaggag ccccaggtga tcatgaccat gccgctgctg gtgggccttg acggcgtgca
     1921 gaaaatgagc aagtccctgg ggaactacat tggaattaac gagccgccga aggagatctt
     1981 cggcaaggtg atgtccatct cggacgacct catgttctgg tactacgagc tcgtcaccga
     2041 cgttcccctc gacgaggtcg cgggcctcaa ggcgggcatc gccgatggga gcgtccatcc
     2101 gcgggacgtg aaggttcggc tcggcaagga gatttgcgcc cagttctacg atcgtgagac
     2161 ggcggacgcc gccgaggcgg aattcaaccg gattttcgtt cgcaaggacc tgccggatac
     2221 catcgaagag tatgccatac cggagtccga gaaatcggac gggaaaatct ggatcgtgaa
     2281 gcttctggtg ctttccggca tggcgaaatc gaacggcgag gccaggcggc tcatttccgg
     2341 cgggggcgtg tacatcgaca gcgaccgcgt cgagaaggac gattacgagc ttccgctccc
     2401 cgcaacgtgc gtcatcaagg ttgggaagcg tcgtttcatg aaaatcaccg gataataaaa
     2461 tagtgtgtaa ccgtcggata aactgaatgt ctgattattc tgatgatgcc gtgatcgccc
     2521 gcgtgcttgc gggagacgcg gacgccttcg aggagattat caaacgctac aacgataagc
     2581 tcttccgata cgtatactcg aaggtgaata attatgacga ggcggccgac gtgacccagg
     2641 atattttcgt catggcctat gagtcgctgg cgtcgttcag gg
//
LOCUS       DAORHF010000389         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_45685_length_2660_cov_4.895202, whole genome shotgun sequence.
ACCESSION   DAORHF010000389 DAORHF010000000
VERSION     DAORHF010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45685_length_2660_cov_4.895202"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="PKJ16_18855"
     CDS             complement(<1..2022)
                     /locus_tag="PKJ16_18855"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HNV49108.1"
                     /translation="MRWFIHLVIAAVVVGAAIPSVAAESKELQRLMEGNARFVAGTTL
                     ARGAIADRRTELTKGQHPFATILTCSDSRVPPELIFDQGLGDVFIVRAAGNVVDRIAI
                     GSIEYGVEHLHTPLLVILGHTSCGAVKATLEQSGAPHGNIGAIVKKIVPAVTEARTHA
                     HGEKAVLDEAIRINMKNVYAEILAKSPVIAELVKGKKLTVVLGVYDLKSGGVSLVEAD
                     DMASRTASALGSFFTGLSLSTVLIIVLSAMVALLLVLYVKKKPEEEAENADEQVGALE
                     TGLHGNSHIRNRSWENSRRAENEKRGGGIMSWFNNLKVGRKVFLSCMIFILIIVGIAG
                     EGFLSQQTASNDFHEFYKYRFVPVRQLNRIMRNLLQIRVNMVQELIAAEEGNIVEVEK
                     RFESSMKLAEEYQKLWEQFTSSKLTPEEKKMADEWVTLGKEPREIRTKYGDAIKRKDL
                     KTAKVYLAQWAAAYQKLRDKTDELINLQQTEGDKLMAEADRSFRASIILSIILLAASI
                     VIGIIITMVLARAVSGPVNKGLAFAQMIAKGDFTSRIDLNQKDELGELGSALNTAADN
                     LENLISNLIVASQNLAQAVEQIASGNQNLSQRTSEQASSLEEIASTIEEATANVKQNT
                     ENSLEANKLAGASSQLAEDGGRVTSEAVASINEINQASKRIGEITSVINE"
     gene            complement(2038..2406)
                     /locus_tag="PKJ16_18860"
     CDS             complement(2038..2406)
                     /locus_tag="PKJ16_18860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49109.1"
                     /translation="MKVYIVEDAPLIRGILIDQLRNMKSIELCGFSHESALAIGDIRE
                     IRPDLVVLDIQLYNGNGIEVLKEFQFDPARPTFVVLTNYPYAHYRERCRQLGVDYFFD
                     KSLELSQAIDVIRERAGAVA"
BASE COUNT          494 a          850 c          709 g          607 t
ORIGIN      
        1 ctcgttgatg accgaggtga tctcgccgat gcgcttgctc gcctggttga tctcgttgat
       61 ggaggccacc gcctcgctcg tgacgcgccc gccgtcctcc gcgagctgcg acgaggcccc
      121 cgcgagcttg ttcgcctcga gggagttttc cgtgttctgc ttcacgttgg ccgtcgcctc
      181 ctcgatcgtg gaggcgatct cctccaggga cgaggcctgc tcgctggtgc gctgcgagag
      241 gttctggttg ccgctggcga tctgctccac ggcctgcgcg aggttctgcg aggccacgat
      301 gaggttcgag atgaggttct ccagattgtc ggccgccgtg ttgagcgcgc tcccgagctc
      361 accgagctca tccttctggt ttaagtcgat gcggctggta aaatcgccct tcgcgatcat
      421 ctgcgcgaaa gcgaggccct tgttcaccgg gcccgacacc gcgcgcgcga gcaccatggt
      481 gatgatgatg ccgatgacga ttgaggcagc gagcagaata atcgaaagaa tgatcgatgc
      541 acgaaatgac cggtctgcct cggccatgag tttgtcgccc tcggtctgct ggagattaat
      601 gagctcatcg gttttatcgc gaagcttctg atacgcggcc gcccactggg cgagatacac
      661 cttggccgtt ttcaggtcct tgcgttttat cgcgtctcca tacttggtgc ggatctcgcg
      721 cggctccttg cccagcgtta cccactcatc ggccattttt ttctcttccg gggtgagctt
      781 cgaggacgta aactgctccc acagcttctg gtattcctcg gcgagcttca tggacgattc
      841 gaaccgtttc tccacctcga cgatgtttcc ctcttcggcg gcgatgagct cctggaccat
      901 gttcacccgg atttgcaaca ggttccgcat gattcggttc aactgccgca cgggcacgaa
      961 gcggtactta taaaactcat gaaagtcgtt ggatgccgtc tgctgcgaaa ggaatccctc
     1021 gcccgcgatc ccaacgataa tcaggatgaa tatcatgcac gacagaaaga ccttgcgtcc
     1081 cacctttaag ttattgaacc agctcataat cccccctcct cgtttttcat tttctgccct
     1141 ccggctgttt tcccaactcc tgttacgtat gtggctatta ccgtgcagtc ctgtttcgag
     1201 agcgcctacc tgctcgtcag cgttttccgc ttcttcttcg ggcttttttt ttacgtagag
     1261 aaccaacaaa agcgccacca tggcgctcag cacgataatg agcaccgtcg acagcgaaag
     1321 tcccgtaaag aacgagccga gcgccgaggc cgttcgcgac gccatgtcgt cggcttccac
     1381 cagcgacacg ccgccgctct ttagatcata gacgcccagc acaaccgtaa gctttttccc
     1441 cttcaccaac tccgcgatta ccgggctctt cgccaggatc tcggcgtaca cgttcttcat
     1501 gttaatgcgt atcgcctcgt cgagcaccgc tttctcgcca tgcgcatgcg tcctcgcctc
     1561 cgtgaccgcc gggacgatct ttttcacgat ggcgccgatg ttcccgtggg gcgcgcccga
     1621 ctgttcaagc gtcgccttca ccgcaccgca ggacgtatgc cctaaaatga ccagcagcgg
     1681 agtgtgcaga tgctccacgc catattcgat gctgccgatg gcgatcctgt ccaccacatt
     1741 gcccgccgcg cggacgataa agacgtctcc gagcccctgg tcgaagataa gctccggcgg
     1801 gacgcgcgaa tccgagcagg tgaggatcgt cgcaaaggga tgctgtccct tggtgagctc
     1861 cgttcgtcga tcggcgatgg cgccacgggc aagcgtcgtt cccgcgacaa atcgcgcatt
     1921 gccctccatg agccgttgca gctctttgga ttcggccgcg actgagggga tggccgctcc
     1981 aaccacgacc gccgcaataa ccagatgaat gaaccacctc atggcatcct ccacacccta
     2041 ggcgacagcg cccgcccgct cccggatcac gtcaatcgcc tgggagagct ccagcgactt
     2101 gtcgaagaaa taatcgacgc ccaactggcg gcatcgttct cggtaatgcg cgtatggata
     2161 gttggtaagc acgacaaacg tggggcgcgc cggatcgaac tggaactcct tgagcacctc
     2221 gatcccgttg ccgttgtata attgtatgtc caggacaacc aggtcggggc gaatttcacg
     2281 gatatcgccg atcgccaatg ccgactcgtg cgaaaacccg cacaactcaa tgctcttcat
     2341 gttcctgagc tggtcgatga ggatcccccg gataagcggc gcgtcctcga cgatatacac
     2401 tttcatgggc tccctccccg tgtgcgttcc cgtctcctgc gctcgtcaac aacatactcc
     2461 gttccgctcc gctcgtatat tgggaggccc cctattttac cgggttcccg gagaaatttt
     2521 taacatagga tttcacctag gcgcggcgta gggaccgacc tactccattc tgtatcgata
     2581 ggcccacctc atccccccgc cccccttctc ccataggaga agggggtgtt cccgacaagt
     2641 agcttgttcc caaggagccc
//
LOCUS       DAORHF010000390         2652 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_45891_length_2652_cov_3.726223, whole genome shotgun sequence.
ACCESSION   DAORHF010000390 DAORHF010000000
VERSION     DAORHF010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45891_length_2652_cov_3.726223"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /gene="dnaX"
                     /locus_tag="PKJ16_18865"
     CDS             complement(<1..544)
                     /gene="dnaX"
                     /locus_tag="PKJ16_18865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HNV49110.1"
                     /translation="MAYQVLARKWRPQDFTGVVHQDHISKTLSSSIRNGRVSHAYLFA
                     GPRGVGKTTMARILAKALNCLSAPGPTDAPCGTCENCVEIRDGSSFDVIEIDGASNRG
                     IENVRELRENVNFAPLKSRFKVYIIDEVHMLTRESFNALLKTLEEPPAHVVFVFATTE
                     IHQIPDTILSRCQKYFFKKIS"
     gene            complement(571..655)
                     /locus_tag="PKJ16_18870"
     tRNA            complement(571..655)
                     /locus_tag="PKJ16_18870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(619..621),aa:Ser,seq:gga)
     gene            complement(724..809)
                     /locus_tag="PKJ16_18875"
     tRNA            complement(724..809)
                     /locus_tag="PKJ16_18875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(773..775),aa:Ser,seq:cga)
     gene            complement(819..1466)
                     /locus_tag="PKJ16_18880"
     CDS             complement(819..1466)
                     /locus_tag="PKJ16_18880"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015404963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HNV49111.1"
                     /translation="MRIILLGAPGAGKGTVAELLIEKYGIPQISTGDMLRSAVKAGTD
                     LGKQAADFMNRGELVPDALIMGLIENRLQEPDARGGFILDGFPRTIEQADQLAAILTK
                     LGMGLDLVVNLEVPEDVIIRRLTSRRTCSNPNCQAIYNIYTKPSQRDGVCDKCGSPTV
                     QRDDETEAVIKKRLSTYQEKTAPLIGYYERNPAYCSVPSLVAGEVIDAIVQRLNR"
     gene            complement(1498..2004)
                     /locus_tag="PKJ16_18885"
     CDS             complement(1498..2004)
                     /locus_tag="PKJ16_18885"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012939900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HNV49112.1"
                     /translation="MAVEQTLVLIKPDGLIKSLTGNILSRLSETRLVIIASKVVRVSR
                     ELAEQHYEHLKNEPFFEELVEYIIGDVHNTHRVMALVYQGDDAIKKVRDIVGHTNPEK
                     ANPVSIRGAYGRITTTGVFENVVHASSSPEDAEKEIKLWFQADEVVGDIYPVKDAVLK
                     EVRQKVWA"
     gene            complement(2432..>2652)
                     /locus_tag="PKJ16_18890"
     CDS             complement(2432..>2652)
                     /locus_tag="PKJ16_18890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49113.1"
                     /translation="TRRVMEVADNISIGTGEQMNANREMTTTINMITESTQSVAESAS
                     QVASSSEQINAQAEALYESIKYFKVTES"
BASE COUNT          496 a          804 c          748 g          604 t
ORIGIN      
        1 ccgagatctt cttgaaaaag tacttctggc agcgggacag tatggtgtcg gggatctgat
       61 ggatctcggt ggtcgcgaac acgaacacca cgtgcgcggg cggctcctcg agggtcttca
      121 ggagcgcgtt gaacgactcc cgcgtgagca tgtggacctc gtcgatgatg tagaccttga
      181 agcgggactt cagcggcgcg aagttcacgt tctcgcggag ctcgcggacg ttttcgatgc
      241 cgcggttcga ggcgccgtcg atctcgatca cgtcgaagga gctgccgtcg cggatctcca
      301 cgcagttctc gcaggtcccg cagggggcgt cggtgggccc cggcgcgctc aggcagttga
      361 gcgccttcgc gaggatgcgc gccatggtgg ttttccccac gccgcgcggg cccgcgaaca
      421 ggtacgcgtg ggaaacccgc ccgttcctga tgctgctcga tagtgtcttc gaaatgtggt
      481 cctgatgaac gaccccggtg aaatcctggg gccgccattt acgggccaga acctggtatg
      541 ccatagtgtg ttgtgcgcgg ttgcgtgatg cggagaggga gggattcgaa ccctcggtag
      601 cttgcgccac acacggtttc caaccgtgct ccttcggcct ctcggaaacc tctcccggaa
      661 cggcgtttcg aacatcatga tacgattgcg cgaaacctgt caaacattgt acgactatcg
      721 ctgcggagag ggagggattc gaaccctcgg tggacaaagc ccacaacggt tttcgagacc
      781 gcccctttcg gccgctcagg cacctctccg attgcgtgct accggttcaa cctctgtacg
      841 atcgcgtcga tgacctcgcc cgccaccagc gaggggacgg agcagtaggc cggattcctc
      901 tcgtaatagc cgatcagcgg cgccgtcttc tcctgatagg tggacagccg ctttttgatc
      961 accgcctcgg tctcgtcgtc gcgctgcacg gtagggcttc cgcacttgtc gcacaccccg
     1021 tcgcgctgcg acggcttggt gtagatattg taaatcgcct ggcagttcgg gttcgagcag
     1081 gtgcgccggc tcgtgaggcg acggatgatg acgtcctcag gcacctcgag attcaccacg
     1141 agatcaagcc ccatgccgag cttcgtcagg atggccgcaa gctgatcggc ctgttcgatc
     1201 gtccggggga agccgtcgag aatgaatccg ccgcgcgcgt ccggttcctg cagccggttc
     1261 tcgatgagcc ccatgatgag cgcatcgggg accagctcgc cccggttcat gaaatctgcg
     1321 gcctgtttcc ccaggtcggt cccggccttc acggcgctgc gcagcatgtc gcccgtcgag
     1381 atctgcggta tgccgtactt ctcgataagc agctcggcaa ccgtgccctt ccccgctccc
     1441 ggcgccccca agagaataat cctcatcgcg accttcctca attcgatgcg tgtggcgcta
     1501 cgcccacacc ttctggcgta cttccttcag tacggcgtcc ttaacgggat agatgtcgcc
     1561 gaccacctcg tcggcctgga accagagctt gatttccttc tcggcgtcct cggggctcga
     1621 cgacgcgtgc accacgtttt cgaacacgcc ggtggtggtg atgcgcccat aggccccgcg
     1681 gatactcacg gggttcgcct tttcgggatt ggtgtgtccc acgatgtcgc gcaccttttt
     1741 gatggcgtca tcgccctgat acaccagcgc catgacccgg tgcgtgttgt gcacgtcgcc
     1801 gatgatatac tcaacgagct cctcgaaaaa cggctcattc ttaaggtgtt cataatgctg
     1861 ctcggcaagc tcgcgcgaca cgcgaacgac cttagaagcg atgataacca atctggtctc
     1921 ggacaggcgc gacaggatgt ttcccgtaag cgatttaata agcccgtcgg gcttgataag
     1981 caccagcgtt tgttcgacgg ccattgcgat gtctccttaa acaaaaaaga ctcttcggaa
     2041 gagtcccatc atatctcgtg cccacgaaca gcgtggggtg aaaataatca acaaattttt
     2101 attggtgctc cgggctgtgc cattcgcact tcgggcactt cacgtcccga tacttgtaaa
     2161 aaccgccatc gagcgttttc acgacctgcg ccatcacaag cgattcgcac gccgggcaat
     2221 gtttcgcaat tacctcatat tcgacattca tctgatactg catacagctt gctcccatct
     2281 cctgaaaaga tcatcgacga acactcccca tgataccgag tgaaggagcg ttcgtatatt
     2341 aaaatccata cgctaccgta gcgcacagat gggcgttcgc cgtattttgt aaagactatt
     2401 tttaattttt tttgattatg tctcctgccg cttacgattc cgtaaccttg aaatacttga
     2461 tgctctcgta cagcgcctcc gcctgcgcgt tgatctgctc ggacgacgag gccacctggg
     2521 aggcgctctc cgccaccgac tgcgttgatt cggttatcat attgatggtg gtggtcatct
     2581 cacgattggc gttcatttgt tcgcccgtgc cgatggagat gttgtccgcg acctccatga
     2641 cccgccgggt ct
//
LOCUS       DAORHF010000391         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_46205_length_2641_cov_4.227378, whole genome shotgun sequence.
ACCESSION   DAORHF010000391 DAORHF010000000
VERSION     DAORHF010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46205_length_2641_cov_4.227378"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="PKJ16_18895"
     CDS             <1..1436
                     /locus_tag="PKJ16_18895"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HNV49114.1"
                     /translation="EQKDMYIPKMMSGEWGGTMCLTEPDAGSDVGALKTKAVKQADGT
                     YKITGTKIFISSGDNDYYKNMVHPVLARIEGDPAGTKGISIFLVPKYLPKADGSIGEF
                     NDVTCAGIEHKMGIKGSATCTLSFGDNGNCKGWLLGEERKGMKIMFQMMNEARLGVGM
                     QGYALASTAYNHAVTYARNRVQGAHVTQMLNPEAKGVTIINHPDVKRMLLWMKSHVEG
                     MRALSYFLTHMLNIEHVADGETKKEALGMAELLIPISKAGNTDMGTLVASEAIQVYGG
                     YGFCSDYPVEQFMRDAKITAIYEGTNGIQSMDLTMRKILMNPEQYNYKTFQNRIANTV
                     AKAKGVVDEKYVALVERGIKKLDELIEMMKAQMAGGKFLHLFMEATQLQQAMHMLALA
                     WVHLWALTIAQPKMKALVGDKKGDDREAFLKDNAEAAYYSGRVLSAQFYIGSEFPKFF
                     GRIEALMGEETAVIKMSDACYTGALME"
     gene            complement(1554..2357)
                     /locus_tag="PKJ16_18900"
     CDS             complement(1554..2357)
                     /locus_tag="PKJ16_18900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HNV49115.1"
                     /translation="MRTIVFTDLDGCLLDHRDYSFDAALPALAELKRRGIPLIIATSK
                     TRAEVTPIRERLGIDHPFIVENGGAIVYPPGAHHLAVEGCVDDDGARIEIRGASYDAL
                     RRFIVEHREEYGITGFGDMTPDAVAALTGLSTEEAARAQKRVFSEPFSLAYERRLPDL
                     AKRAASRGLAILRGGRFYHCVASGHDKGTAARALLERYRGALGGDLRSIAIGDAENDL
                     PLLRAATDPVLLPRSDGVHIACDIPRLFRATQPGPRGWAEAVLALCGGR"
     gene            complement(2367..>2641)
                     /locus_tag="PKJ16_18905"
     CDS             complement(2367..>2641)
                     /locus_tag="PKJ16_18905"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HNV49116.1"
                     /translation="GFVEEAGVVELVIACASAIAIGGALLGWRFALPASAAIASTALY
                     AIFMKKKIGGITGDTLGALNELVETIALASFLALPVLAHTLPAFPV"
BASE COUNT          533 a          816 c          866 g          426 t
ORIGIN      
        1 aggagcagaa ggacatgtac atccccaaga tgatgagcgg cgaatggggc ggcaccatgt
       61 gtctcaccga gcccgacgcc ggttccgacg tgggcgcgct caagaccaag gccgtgaagc
      121 aggccgacgg cacctacaag atcaccggca cgaagatctt catctcctcc ggcgacaacg
      181 actattacaa gaacatggtg cacccggtcc tcgcgcgcat cgagggcgac ccggccggca
      241 ccaagggcat ctccattttc ctggttccca agtatctccc gaaggcggac ggttcgattg
      301 gcgaattcaa tgacgtcacc tgcgccggta tcgagcacaa gatgggcatc aagggctccg
      361 cgacctgcac gctgtccttc ggcgacaacg ggaactgcaa gggctggctc ctgggcgaag
      421 agcgcaaggg catgaagatc atgttccaga tgatgaacga ggcccgtctg ggcgtcggca
      481 tgcagggcta cgcgctggcg agcaccgcgt acaaccacgc cgtcacctac gcgcgcaacc
      541 gcgttcaggg cgcgcacgtc acgcagatgc tcaacccgga agcgaagggc gtgaccatca
      601 tcaaccaccc cgacgtgaag cgtatgctcc tgtggatgaa gtcgcatgtc gaggggatgc
      661 gcgcgctgtc gtatttcctc acgcacatgc tcaacatcga gcacgtggcc gatggcgaga
      721 ccaagaaaga ggcgctgggc atggccgagc tcctgattcc gatctcgaag gcgggcaaca
      781 ccgacatggg gacgctggtc gcctccgagg ccattcaggt gtacggcggg tacggcttct
      841 gctccgacta tccggtcgag cagttcatgc gcgacgcgaa gatcaccgcg atctacgaag
      901 ggaccaacgg catccagtcg atggacctca ccatgcggaa gatcctcatg aaccccgagc
      961 agtacaacta caagaccttc cagaaccgca tcgcgaacac cgtcgccaag gcgaagggcg
     1021 ttgtggacga gaaatacgtc gctctcgtgg agcgcggcat caagaagctc gacgagctca
     1081 tcgagatgat gaaggcgcag atggccggcg ggaagttcct ccacctgttc atggaggcca
     1141 cccagctgca gcaggcgatg cacatgctgg cgctggcgtg ggtgcacctc tgggcgctca
     1201 ccatcgcgca gcccaagatg aaggccctcg tgggcgacaa gaagggcgac gatcgcgagg
     1261 ccttcctgaa ggacaatgcg gaagccgcct actacagcgg ccgcgttctc tcggcgcagt
     1321 tctatatcgg ctccgagttc ccgaagttct ttggccgcat cgaggcgctg atgggcgaag
     1381 agaccgccgt catcaagatg tccgatgcct gctacaccgg ggcgctcatg gaatagccgt
     1441 accgatcgca tacgcagcag tacccacaca agaaggcggg gatattcccc gccttcttgc
     1501 atttcgggag ccgctggacg tgcagcggat cgatgccggg cgtatcgcgc cggctaccgt
     1561 ccgccgcaaa gcgccaggac cgcctcggcc catccgcgcg gtcccggttg cgttgcgcga
     1621 aacaaacgcg ggatgtcgca ggcgatatgg acgccgtcgc ttcggggcag gaggacgggg
     1681 tcggtggcgg cgcgaagcag cgggagatcg ttttcggcgt cgccgatcgc gatacttctg
     1741 aggtcgccgc cgagcgcgcc gcgatagcgc tccaagagcg cacgtgccgc ggtccccttg
     1801 tcatgtcccg aggccacaca atgataaaac cgcccgccgc gcaggatggc aaggccgcgc
     1861 gatgccgccc gcttggcgag gtccggaaga cggcgctcat acgccagtga aaacggttcg
     1921 gagaatacgc gcttttgtgc acgggcggcc tcttcggtcg aaagtcccgt gagcgcggcg
     1981 accgcatcgg gcgtcatgtc cccaaagccg gtgatgccgt attcctcacg gtgctcgacg
     2041 atgaatcgac ggagcgcgtc gtacgaggcg ccgcggattt cgatgcgggc gccgtcgtcg
     2101 tcgacgcatc cctcgacggc cagatgatgc gcgcccggcg ggtagacgat cgcgccgccg
     2161 ttttccacga tgaacggatg gtcgatcccg aggcgctcgc ggatcggcgt cacctcggcg
     2221 cgcgttttgc tggttgcgat gatgagcggt atcccgcggc gtttcagctc ggcaagggcg
     2281 gggagggccg cgtcgaacga gtaatcgcga tgatcgagaa ggcagccgtc gagatcggtg
     2341 aagacgatcg tgcgcatggc ggatcgttac acgggaaatg cgggaagggt gtgtgcgagc
     2401 accggcagcg caaggaagga ggccagtgcg atggtctcga caagctcgtt gagcgcgccc
     2461 agcgtgtcgc cggttatccc gccgattttt ttcttcatga agatggcgta caacgcggta
     2521 cttgcgatgg ccgccgatgc gggaagcgcg aagcgccaac cgagaagcgc accgccgatg
     2581 gcgatcgcgc tcgcgcacgc gatgacgagt tcgacgacgc ccgcctcttc gacgaaaccc
     2641 t
//
LOCUS       DAORHF010000392         2608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_47101_length_2608_cov_4.106541, whole genome shotgun sequence.
ACCESSION   DAORHF010000392 DAORHF010000000
VERSION     DAORHF010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47101_length_2608_cov_4.106541"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="PKJ16_18910"
     CDS             <1..1038
                     /locus_tag="PKJ16_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49117.1"
                     /translation="GDIMRYRGHSIPARIVIIACAIACVPTMARADDSLSPRRLALKL
                     LQQYSPSGYYIISQYAAARTSFTCESGSVSVSKTDFSSYLRGTAPAQIAESLNTAVHE
                     ICHGYTGLLAYPAKEAAGIGACASGTYFSYYIGGTESVLVKQTPVFPTREMAASIPAR
                     MHTLRFPTYVLASGNLGAQVNGIYGLLDELNAYYHGTKTAFDLLTFYRTEMPQTPETW
                     LAFFTGVNGTYYAYGEFTYYISMYLRHAKANHPAQYREIVANESLKNAYAKIDRNYRD
                     LIAAYVAAKKDILAELARQGYSVSEDSKLLWIGKNGSRQGRGNFLDVHTRLMDELAKD
                     EHRTMLNALRP"
     gene            1044..1775
                     /locus_tag="PKJ16_18915"
     CDS             1044..1775
                     /locus_tag="PKJ16_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49118.1"
                     /translation="MSIRTASIRMARQTLTVCALAGALAAAPVRSAVPEQEAVFSALR
                     ARVAESNRKYIGNESRRAITTHEYDAKTGALISSSEALVHRKEYYHRKPVNTPIKYRK
                     NGIELPPEEFKYRSRDPVHLPFDTTDGEQYRYRIIGRKTVEGRECHAIEIIPIKNTAR
                     HLRAVLYVTVDTIEPQYLEGTIADRPLGVSDIRVRLSFATVRGESIMRRGTYVIMLNI
                     PMLYPHRRIVSSFVSSDERPIPKED"
     gene            1781..2401
                     /locus_tag="PKJ16_18920"
     CDS             1781..2401
                     /locus_tag="PKJ16_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49119.1"
                     /translation="MPQRAFGIVALLFACSLTTSGCDGDLIDIDAVVRGASLDRARES
                     SVDEFFSRRMTNRPRKAAVGSWQILHENDRSVYYAYPRFTSLLGSRRAADALYRTGRS
                     ELVRRFPAYRNIAGNDVRLAVFGAIGAHRTALRPRDESLFLGWEWSARLATDGIHVDA
                     VAGVTRAGVPSRVRYLVRLNATTLKVLAISERPLDQKTPESSDDLI"
     gene            complement(2394..>2608)
                     /locus_tag="PKJ16_18925"
     CDS             complement(2394..>2608)
                     /locus_tag="PKJ16_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49120.1"
                     /translation="VVFGLKAVALQKRDEVGAVDEDCRTHFGFLLSGMRNYSDTVIFL
                     HSHGIVKLNMFHHRAGREIWMQHGVK"
BASE COUNT          535 a          891 c          714 g          468 t
ORIGIN      
        1 ggtgacatca tgcgatatag aggccattct ataccagcgc gcattgtcat tatcgcgtgt
       61 gcgatagcat gcgtaccgac gatggcccgc gccgacgatt cactctcccc gcgacgtctg
      121 gcgctcaagc tccttcagca gtattcgccc tcgggatatt acatcatcag ccaatacgcc
      181 gccgcgcgaa catcgttcac ctgcgaatcc gggagcgtct ccgtctccaa aacggatttc
      241 tcctcgtacc tccgcgggac agcacccgca cagatcgccg agtcgctgaa taccgccgta
      301 catgaaatct gtcacggcta caccggcctc ctcgcctatc ccgcgaaaga ggcggccggc
      361 atcggcgcct gcgcgtccgg gacttacttc agctattaca tcggcgggac ggaatcggtc
      421 ctggtgaaac agacccccgt gttccccacc cgggaaatgg ccgcgtcgat cccggctcga
      481 atgcacacgc tccgctttcc cacctacgtc ctggcgagcg gaaatctggg cgcccaggtc
      541 aacggcatat acggcctcct ggacgagctc aacgcctatt atcacggcac aaaaaccgcc
      601 ttcgacctgc tgacgttcta ccgaacggag atgccccaga ccccggagac ctggctcgcc
      661 ttctttaccg gcgtaaacgg gacctattac gcctatggcg aattcaccta ctacatctcg
      721 atgtacctca ggcacgcgaa ggcgaatcat cccgcgcagt accgggagat tgtggccaat
      781 gagtccctta aaaacgcata cgccaagata gaccgcaact atcgggacct cattgccgcc
      841 tacgttgccg ccaaaaagga cattctggcc gaacttgcgc gccagggata cagcgtctcg
      901 gaggacagca agcttctctg gataggtaaa aacggttcgc ggcaggggcg cggcaacttc
      961 ctcgatgtcc atacgcggct catggacgag ctggccaagg acgagcaccg aacaatgctg
     1021 aacgcattgc gcccgtgacg ctcatgtcga tccgcaccgc gtcgatacgg atggcccggc
     1081 aaaccctgac ggtgtgcgca ttggccggcg cgctcgcggc cgcgccggtg cgctccgcgg
     1141 tccccgagca ggaggcagtc ttctccgcac tgcgggcgcg ggtggcggaa tcgaaccgga
     1201 agtacatcgg gaacgagtcg cgacgcgcca tcaccacgca cgaatacgac gcaaaaaccg
     1261 gcgcgctgat cagcagctcg gaggcgctgg tgcaccggaa ggagtactat caccggaaac
     1321 ccgtcaacac gcccatcaaa taccgtaaaa acgggatcga actgccgccg gaggaattca
     1381 agtatcgctc gcgagatccc gtccaccttc ccttcgatac gaccgacggc gagcagtacc
     1441 gctaccgcat catcggtcgt aaaaccgtcg agggccggga gtgccatgct atcgagatca
     1501 ttccgattaa aaacaccgcg cgccatctcc gggccgtcct gtacgtcacc gtcgacacga
     1561 tcgagccgca gtatctcgaa ggaaccatcg cggaccgacc gctgggggtg agcgacatac
     1621 gggtgcgcct ctcgttcgcg acggtgcgcg gcgagtccat catgcggcgc ggcacctacg
     1681 tcatcatgct caacatcccc atgctctatc cgcaccgccg catcgtcagc tcgttcgtct
     1741 catcggacga acggccgatc cccaaggaag actgaccgcc atgccccaac gcgcattcgg
     1801 gatcgtcgca ctccttttcg cctgctcgct cacgacgtcg ggctgcgatg gcgacctgat
     1861 cgacatcgac gcggtcgtgc gcggcgcatc gctcgatcgc gcgcgggaga gctccgtgga
     1921 cgagtttttc tcgcgacgca tgaccaaccg cccgcgcaag gccgcggtgg gcagctggca
     1981 gatcctccac gagaacgatc ggtccgtata ttacgcgtat ccccggttca catcgctcct
     2041 gggctcccgg cgggccgccg acgccctcta ccgcaccggg cgctcggagc tcgtgcgtcg
     2101 atttcccgcc tatcgaaaca tcgcgggcaa cgacgtccgg ctggcggtct ttggcgccat
     2161 cggagcgcat cgcacggccc ttcgcccccg cgacgaatcc ctctttctgg gatgggaatg
     2221 gagcgcccgc ctggccaccg acggcatcca tgtcgatgcc gtcgccggtg tcactcgcgc
     2281 gggcgtgccg tcccgagtgc gctacctggt ccgcctgaat gcgaccacgc tcaaggttct
     2341 cgcgatctcc gagcgccccc ttgaccagaa aacccccgaa tcctccgacg acctcatttg
     2401 acaccgtgct gcatccatat ttcgcgccct gcacggtgat ggaacatatt caatttgaca
     2461 atcccatgcg aatgtaaaaa aatgactgta tccgaatagt tccgcattcc actcaggagg
     2521 aaaccaaaat gcgtgcggca atcttcatcg acggcgccta cctcatctct tttctgaagc
     2581 gccacggctt taagcccaaa tacgacga
//
LOCUS       DAORHF010000393         2575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_48035_length_2575_cov_5.539286, whole genome shotgun sequence.
ACCESSION   DAORHF010000393 DAORHF010000000
VERSION     DAORHF010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48035_length_2575_cov_5.539286"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..1180
                     /gene="rho"
                     /locus_tag="PKJ16_18930"
                     /pseudo
     CDS             1..1180
                     /gene="rho"
                     /locus_tag="PKJ16_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465982.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
     gene            1290..1490
                     /gene="rpmE"
                     /locus_tag="PKJ16_18935"
     CDS             1290..1490
                     /gene="rpmE"
                     /locus_tag="PKJ16_18935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HNV49121.1"
                     /translation="MKPDIHPEYVDTVIKCACGNEIKTRSTAKDLRVEVCSACHPFYT
                     KKQRIVDSTGRVEKFKKKYKIK"
     gene            complement(1637..1909)
                     /locus_tag="PKJ16_18940"
     CDS             complement(1637..1909)
                     /locus_tag="PKJ16_18940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49122.1"
                     /translation="MQNTKGFWCVQMGLVALLYGVVLFHAGALFPCWPWAGPVGVVLM
                     MLFHASEIPIARKALAGSGIPRARQILLIVLFGYTWWGGAKRGVLG"
     gene            1993..>2575
                     /locus_tag="PKJ16_18945"
     CDS             1993..>2575
                     /locus_tag="PKJ16_18945"
                     /inference="COORDINATES: protein motif:HMM:NF017200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HNV49123.1"
                     /translation="MLSKTISSGICGIDAHIVEVEVDIVAGLPNFTIVGLPDSTIKES
                     KTRIRSAIENSGFEFPPSNYAVNLAPAWVRKQGVAFDLPIAIALLRASGQVAAPAHDT
                     PMVGEISLDGGIKPVHGVIAMAIAFYRSGYRRMIVPFENRFEAAAVGEIAVFPVRSIG
                     EVIRVLAGEAEPFVAGDPAPHDDGAAHDFADVRG"
BASE COUNT          529 a          814 c          801 g          431 t
ORIGIN      
        1 gtcgaggggg tcacgggcct caagtaacag gagctcatct tcgagatcct gaagacgcag
       61 accgagcgaa acggccttat cttctccagc ggcgtgatcg aaatccaacg acggctacgg
      121 tttcctgcgt tcgcccaatt acaactacct cccgggcccc gacgacatct acgtatcgcc
      181 ctcgcagatc cggctcttcg gcctgcgcac cggcgacacc gtgaccgggc aaatccgccc
      241 gcccaaggac aacgagcgct tctacgccct gctgcgcgtg gaggcggtga acttcgagga
      301 cccggagaac ctgcagcgcc gcgtgttgtt cgacaacctc acgccgctct atccggacaa
      361 gcgcatcgag cttgagaccg tgaccgagaa catcgacatg cgcatcatga acatcgtgac
      421 gccgctgggc aaggggcagc gcgcgctgat cgtggcgccg ccgcggacgg ggaagacgat
      481 cctgctccag aagatcgcga actccatcac caacaatcac cccgaggtga tcctgctggt
      541 gctcctgatc gacgagcggc cggaggaggt gaccgacatg tcgcgctccg tgcggggcga
      601 ggtcatatcg tccacgttcg acgagccggc gtcgcggcac gtgcaggtgg ccgagatggt
      661 gatcgaaaag gccaagcgcc tcgtcgagca ccacaaggac gtggtgatcc tgctcgattc
      721 gatcacccgc ctggcccggg cctacaacca ggtggtcccc accagcggca agatcctctc
      781 cggcggcgtg gactcgaacg cgctgcacaa gcccaaacgg ttcttcggcg cggcgcgcaa
      841 catcgaggag ggcggcagtc tcaccatcat cgccaccgcg ctcatcgaca ccgggagccg
      901 catggacgag gtcatcttcg aggagttcaa gggcaccggc aactcggagc tccacctcga
      961 ccgcaagctg gtagaccgcc gaatcttccc cgcgatcgac atgaacaagt cgggcacgcg
     1021 caaggaggaa ctcctcgtgg agccggagga gctcaacaag atatgggtgc tccgcaaggt
     1081 catctcgggc atgagcccca ccgaggccat ggagctttta gtggaaaaga tgcgcgcgac
     1141 caagagcaac cgggccttct ttaaggccat gaatgcctga tcgccgtccg gcgtttcccg
     1201 gaaaatgccg gggacgggaa aaaaatgttg aataatttgg cgggtatata accctgcgcc
     1261 atgcgacatc agcagtgtgg agatttgcca tgaaacccga tattcatccc gaatacgtgg
     1321 acaccgtgat caagtgcgcc tgcggcaacg agatcaaaac ccgctcgacc gctaaggacc
     1381 tgcgcgtgga agtgtgctcg gcctgccatc cgttctacac caagaagcag cgcatcgtgg
     1441 acagcaccgg ccgcgtcgag aagttcaaga agaagtacaa gatcaagtag gcccgagacc
     1501 gccgacatcg tgcacgcatc cggacccctg ccctggcagg ggtccgctcg tcatagagtg
     1561 cggatttctc gatatagatc tcttcgctcg ctgcaacggc gagtgaaaca gtacggcaaa
     1621 gttatattcc ctcgggctac cccagcacgc cccgcttcgc cccgccccac caggtgtagc
     1681 cgaacaggac gatgagcagt atctgtcgtg cgcgagggat gcccgaaccg gcaagcgcct
     1741 tccgtgcaat cgggatctcg gaggcgtgga agagcatcat gagcacgacc ccgacggggc
     1801 cggcccaggg ccagcaggga aacagcgcgc ccgcgtggaa gagcaccacg ccgtagagga
     1861 gcgccaccag gcccatctgc acgcaccaga atccctttgt gttttgcatc gtcgttctcc
     1921 gcggtttaaa tttttccgtc ccgggttcgt attaatgatg ttgatcatca tcatacgaac
     1981 ggggacggcg acatgttgtc aaagacaatt tcttcgggca tctgcgggat cgacgcgcac
     2041 atcgtggagg tggaggtgga catcgtggcg ggcctgccca atttcaccat cgtgggcctg
     2101 cccgattcca ccatcaagga atcgaagacg cggatccgtt ccgccatcga gaattccggg
     2161 ttcgagttcc cgccgagcaa ttacgcggtg aacctcgcgc cggcatgggt gcgcaagcag
     2221 ggcgtggcct tcgatctccc catcgcgatc gcgctcctgc gggcgagcgg gcaggtggcg
     2281 gccccggcgc acgacacgcc gatggtcggg gagatctcgc tggacggcgg catcaagccc
     2341 gtgcacgggg tgatcgccat ggccatcgcg ttttaccggt ccggctaccg gaggatgatc
     2401 gtcccgttcg aaaaccgatt cgaggccgcg gcggtgggcg agattgcggt gttcccggtg
     2461 cgaagcatcg gcgaggtgat ccgcgtgctg gccggcgagg ccgagccgtt tgtcgcgggc
     2521 gatcccgcgc cgcacgacga cggcgccgcg cacgatttcg ccgatgtgcg cgggc
//
LOCUS       DAORHF010000394         2553 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_48695_length_2553_cov_5.122498, whole genome shotgun sequence.
ACCESSION   DAORHF010000394 DAORHF010000000
VERSION     DAORHF010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48695_length_2553_cov_5.122498"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="PKJ16_18950"
     CDS             complement(<1..261)
                     /locus_tag="PKJ16_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49124.1"
                     /translation="MKVVVLLMIVFALGCGKKALFDELAENRLKVVIKGTYESNDPRS
                     WLGAFPDDNSIDDGIANTELPTRLMLDIAEIQLLDKEGGVEPF"
     gene            complement(272..841)
                     /locus_tag="PKJ16_18955"
     CDS             complement(272..841)
                     /locus_tag="PKJ16_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49125.1"
                     /translation="MRKLFIAACVMCMVLVSLSAHARQGRKKDPFTKFQAGFWFGPIT
                     PVYTTADDVNKSLGGGIFLRSLTFLDSFKLGIDASYEKFESRGVNKLILWPVYGSLLW
                     RIPLKTPLVIQLKAGGGGCYVTIYPDRASQWDPMFMLGGEVSFPAGKWVNIGLRIEYL
                     CIYENHIPGSVRNGHIVNAGISLYFNFGS"
     gene            complement(986..1789)
                     /gene="truA"
                     /locus_tag="PKJ16_18960"
     CDS             complement(986..1789)
                     /gene="truA"
                     /locus_tag="PKJ16_18960"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HNV49126.1"
                     /translation="MEPAGGPAGSDRIALTIQYDGTRFEGSQIQARGRTVQGELERAL
                     ELLTKRHIRLIFSSRTDSGVHAIEQVAHFEGPPGIALSRLCIGLNGIAERDVSIRNAY
                     RVPADFHARYSAVEREYIYCIHNHPNRSPFSRYRAMWVREPLDVDYLRCALGPLTGEM
                     DYAAFCKKKSAEAERSTVRRISHADVARWDDYIIVVIRGNAFLHNMIRVIVGTMISLH
                     REGRDPEHMRSVLSSASRMAAGFTAPPYGLYLNRVIYDPPLSSMEAAFK"
     gene            complement(1750..>2553)
                     /locus_tag="PKJ16_18965"
     CDS             complement(1750..>2553)
                     /locus_tag="PKJ16_18965"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HNV49127.1"
                     /translation="EEMLTGGGRLIAGKLTDELRRLYDKNEKFGKVYPMAYVLTVCPR
                     CLYTAYPKDFGILTDEEKKKMQGTAQARINSVKKFFGDIDFREDRSLVLGAASYLLAV
                     DCYSFRRKEVAPTFKNAMSSIRAAWLFGDLAREVPEKPYGKISQFFYKKSYAYYMQAI
                     DLAQTGGETLEAAGHIGPDTDKNWSYEGLLYMCAILTVKVGAQEPDLAKRLESFEKTK
                     RFLSRLFGVGKSSKSKPGALIDMTRDLYERMNAMIEEWNQQAGQPDQTE"
BASE COUNT          586 a          776 c          649 g          542 t
ORIGIN      
        1 gaacggctca acaccccctt ctttatcgag cagctgtatc tcggcaatat caagcatcag
       61 gcgcgtcggc agctcggtat tcgcgattcc gtcatcgatc gaattatcat ccgggaatgc
      121 gcccagccag ctgcgtggat cattcgactc ataggtcccc ttgattacaa ccttgagtcg
      181 attttcggcc agctcgtcga acagggcctt cttcccgcag ccaagcgcga aaacaatcat
      241 taacaatacg accactttca tgacattcgc atcagctccc gaaattgaaa tacagactga
      301 tcccggcatt gacgatgtgt ccgttgcgaa ccgagccggg tatgtgattt tcgtaaatgc
      361 acagatactc gatccgaaga ccgatattga cccactttcc cgccggaaag gaaacctctc
      421 ctccgagcat gaacatcggg tcccactggc tcgcccggtc cggatagatc gtcacatagc
      481 agccaccccc gcccgccttc agctgaatca ccagcggggt tttcagcgga atgcgccaca
      541 gcagacttcc gtacaccggc cagaggatga gcttgttaac gccgcgcgat tcgaattttt
      601 cataggacgc gtcgataccg agtttaaacg aatcgagaaa cgtcaacgat cgtaaaaaga
      661 tgccgccccc aaggcttttg ttcacgtcat cggcggtcgt gtacacggga gtgatcggcc
      721 cgaaccaaaa ccccgcctgg aatttcgtaa acgggtcctt cttccgcccc tgccgggcat
      781 gcgccgagag cgatacgagc accatgcaca tgacgcaggc agctatgaaa agttttctca
      841 tcacccgttc gatttacaag aatccgatat gttacaggaa aaagcatccc cagggagaag
      901 gccgggcagg atgcgccccc tcattatggg acttacagca atccgcgcaa cggttttgtc
      961 aaataaaaat acccgcactc gcgaattatt taaacgcagc ctccatcgag gaaagcggcg
     1021 gatcgtatat cacgcgattg agatacagcc catagggcgg ggcggtgaat cccgcggcca
     1081 tgcggcttgc cgaggagagc accgaccgca tgtgctccgg gtcgcgtccc tcgcgatgca
     1141 acgaaatcat cgtccccacg atgacgcgga tcatattgtg aagaaaggca ttgcccctga
     1201 taacgacaat gatataatca tcccaacgcg caacatcggc atgggaaatc cgtcgaacgg
     1261 tcgaacgctc cgcttccgct gatttctttt tacaaaaggc ggcgtaatcc atttcgccgg
     1321 taagcggccc aagcgcgcat ctgagataat cgacgtcgag cggctcgcgg acccacatgg
     1381 cgcggtaacg ggaaaacggg ctgcgattgg gatgattatg gatgcagtat atatactcgc
     1441 gctcgacggc gctatagcgc gcatgaaaat cagcgggtac gcgataggcg tttcgtatcg
     1501 aaacatcgcg ctcggcaata ccgttgagcc cgatgcacaa ccggctgagc gcgataccgg
     1561 gcggcccctc gaaatgcgca acctgctcga tcgcatgaac gccggaatcg gtcctactgg
     1621 aaaagatcaa ccgaatatgc cgcttcgtca gaagctcgag cgcccgctcc agctctccct
     1681 gcaccgtccg cccgcgcgcc tgtatctgcg aaccctcgaa acgcgttcca tcgtactgga
     1741 tcgtgagcgc tattcggtct gatccggctg gcccgcctgc tggttccatt cctcaatcat
     1801 cgcattcatc cgttcataca gatcgcgcgt catgtcgatg agggcgcccg gtttcgactt
     1861 ggacgatttc cccacgccaa agagacggct caggaagcgc tttgtctttt caaagctctc
     1921 gagccgtttg gccagatccg gctcctgcgc accgaccttc acggtaagga tggcgcacat
     1981 atacaggagc ccctcatagc tccagttttt atccgtatcc ggaccaatgt gcccggcggc
     2041 ctcaagcgtc tccccgccgg tttgcgcgag atcgatcgct tgcatgtagt acgcatacga
     2101 cttcttgtaa aaaaactgcg atatcttccc gtagggcttt tccggaacct cacgcgcgag
     2161 atcgccgaag agccacgcgg cgcggatcga cgacatcgcg tttttaaacg tgggggccac
     2221 ttccttgcgc ctgaaactgt aacaatcgac ggcaagcaaa tatgatgccg cgccgaggac
     2281 cagcgagcgg tcctcccgga aatcgatatc accaaaaaac ttttttactg agttgatccg
     2341 cgcttgcgcg gtcccctgca tcttcttttt ttcttcgtcc gtcagtatcc caaagtcttt
     2401 cggatacgcg gtatacaggc agcgcggaca cacggtcagc acatatgcca ttgggtacac
     2461 cttgccgaac ttctcgtttt tatcgtagag gcgccgcagt tcgtcagtca gcttgcccgc
     2521 gatgagccgc ccgccgccgg taagcatctc ctc
//
LOCUS       DAORHF010000395         2535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_49294_length_2535_cov_5.489919, whole genome shotgun sequence.
ACCESSION   DAORHF010000395 DAORHF010000000
VERSION     DAORHF010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49294_length_2535_cov_5.489919"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /locus_tag="PKJ16_18970"
     CDS             complement(<1..1905)
                     /locus_tag="PKJ16_18970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HNV49128.1"
                     /translation="MSAASIGPYLIIEKVGESHKSVVYRCRGRGADETVIVKLLKKAA
                     PTPADVARIRLESALVERLDFDGIVRGRGIIEHEGAIALVLEDPHGISLKDFISRTRP
                     TLRQFLDIAVRLARILGLIHRANIIHKDIKPHNIIIRTARGRVRDVHITDFGISVLLD
                     DGLRELYHPDVLQGTLPYISPEQTGRMNRPVDYRTDLYSLGVTFYELLTGDVPFHADD
                     PLELIHAHMARAPEPPAAVNSALAGPVSDIVMKLLAKSPDERYQNALGLAYDLQECHT
                     RLVRDGRIAPFELSTRDIAERLTVPQVLFGRERERDQLFAAFERVVGGAAEMVLISGA
                     PGIGKTSLAMELHKPVTARSGFFVAGKYDALRRDVPYTAIIQAFQGLIRQILTEGEER
                     IDLWRIRLETALGLHAKIIGDQIPELRLIIGEQPEVAQLDLEESHNRFTLVFRNFMSV
                     FAKPEHPLVVFLDDLQWADSASLTLLATVLSDRNMRSLLLLGAIREGPTVDSLLDQTV
                     STLQTAGVAVSTLSLGPLEPDAVGEYLANFLRVRNRRQQTFAEIVYEKTLGNPFFINQ
                     FIGMLYDDGLIRFDPESGWTWSLGKLRALEVSDNVVELMAKKIGTLPPRTQEALQIAS
                     VIGNRFSLETV"
     gene            2301..>2535
                     /locus_tag="PKJ16_18975"
     CDS             2301..>2535
                     /locus_tag="PKJ16_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HNV49129.1"
                     /translation="MTERMHDSSMPAKSYYKNINEFQLLTQEEEIELARAKTKGDDTA
                     RERLVNANLRLVIKIARNYTMLEPSLVDLIQEGN"
BASE COUNT          505 a          718 c          816 g          496 t
ORIGIN      
        1 caccgtctcg agggaaaaac ggttcccgat caccgaggcg atctgcagcg cctcctgcgt
       61 gcgcggcggg agcgtgccga tcttcttggc catgagctcg accacgttgt cgctcacctc
      121 cagcgcgcgg agcttcccga ggctccaggt ccagcccgat tccggatcga agcggatgag
      181 cccgtcgtcg tagagcatgc cgatgaactg gttgatgaaa aacgggttgc ccagggtctt
      241 ctcgtagacg atctcggcga aggtctgctg gcggcggttg cgcacgcgca ggaagttcgc
      301 caggtattcg cccaccgcgt cgggctcgag cggtcccagc gacagcgttg acaccgccac
      361 cccggcggtc tggagcgtcg agacggtctg gtccagcagc gagtcaacgg tgggcccctc
      421 gcggatcgcg ccgaggagaa ggagcgagcg catgttgcgg tcagaaagga cggtggccag
      481 gagcgtgagg ctcgcgctat cggcccactg gaggtcgtcc aggaagacga ccagcgggtg
      541 ttccggtttg gcgaagacgc tcatgaagtt ccggaacacc agggtgaacc ggttatgcga
      601 ttcctccagg tcgagctggg ccacctccgg ttgctcgccg atgatgagcc gaagctccgg
      661 gatctggtcg ccgatgatct tcgcgtgcag ccccagcgcc gtctccagac ggatccgcca
      721 gaggtcgatc cgctcctcgc cctcggtgag gatttggcgg atgagcccct ggaaggcctg
      781 gatgatggcg gtgtagggga cgtcgcggcg cagcgcatcg tactttccgg cgacgaagaa
      841 gccgctgcgc gccgtcaccg gcttgtgcag ctccatcgcc agcgaggtct ttccgatgcc
      901 gggcgcgccg gagatgagca ccatctcggc ggcgccgccc accacgcgct cgaaggcggc
      961 gaagagctgg tcgcgctccc gctcgcgccc gaagagcacc tgcgggacgg tgagccgctc
     1021 ggcgatgtcc cgcgtcgaca gctcgaaagg cgcgatgcgc ccgtcgcgga ccaaacgggt
     1081 gtggcattcc tgcaggtcgt aggccaggcc cagggcgttc tggtagcgct cgtcgggcga
     1141 tttcgccagg agcttcatca cgatgtccga aaccggcccg gccagcgcgg agttgacggc
     1201 ggcgggcggt tcgggcgcgc gcgccatgtg ggcgtggatg agctccagcg ggtcgtccgc
     1261 gtgaaacggc acatcgcccg tgagcaactc gtagaaggtg acgccgagcg agtacaggtc
     1321 cgtgcggtaa tcgaccgggc ggttcattct gccggtctgt tcgggcgata tgtacggcag
     1381 cgttccctgc agaacgtcgg ggtgatagag ctctcggagc ccgtcgtcca agagcaccga
     1441 gatgccgaag tcggtgatat gcacgtccct gacgcgtccg cgggcggtcc ggatgatgat
     1501 attgtgcggc tttatgtcct tgtggatgat attggcccga tggatgagcc ccaggatgcg
     1561 cgccagcctc acggcgatat ccaggaactg acggagcgtc ggccgggttc gcgagatgaa
     1621 gtccttgagc gatattccgt gcgggtcctc gaggaccagc gcgatcgccc cctcgtgctc
     1681 gatgatgccg cgtccccgga cgatcccgtc gaaatcgagc cgctcgacga gggcgctctc
     1741 cagccggatg cgggcgacat cggccggggt gggcgccgct tttttgagca gcttgacaat
     1801 gacggtctca tccgccccgc gtcctcggca gcggtagacg acggatttat ggctctcgcc
     1861 gaccttttcg atgatgagat acggcccaat ggacgcagcg gacattgatt gatcgcgcct
     1921 cgtagcgaag tgtggcattc atacgcgccg tcaatgacgc tatcggtttc gcgcccgctg
     1981 tcaaatctat ttcgaggcgg ggttttccct cgcggtatag aaatatcata caaaatgata
     2041 ttatagatag aatgcgtgcc gctcctggcg ttttaatgtg caaaattata tttttgaaaa
     2101 caaatcttgt tacagtcagg ttaatttcat tataaaactg taacaatttc aatatttggg
     2161 gtggtcgaat aggtaaacgc tctgtatatt attagggagt aaactaaatc agaatttaat
     2221 ttaaatctta aatatttcaa taacgttcat agtgttgatt gttcatagga gtaataatct
     2281 cggtcgatag gagggaggcc atgacggaaa gaatgcacga tagctccatg ccggcgaaat
     2341 catactacaa gaacataaat gaatttcagc tgcttaccca ggaagaggag atcgagcttg
     2401 cccgggcgaa gacgaagggg gacgataccg cccgcgagcg cctggtgaac gccaacctca
     2461 ggctggtgat taaaatcgcg aggaactaca ccatgctcga accgtccctg gtggacctca
     2521 tccaggaggg gaaca
//
LOCUS       DAORHF010000396         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_49444_length_2530_cov_5.738586, whole genome shotgun sequence.
ACCESSION   DAORHF010000396 DAORHF010000000
VERSION     DAORHF010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49444_length_2530_cov_5.738586"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PKJ16_18980"
     CDS             complement(<1..370)
                     /locus_tag="PKJ16_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49130.1"
                     /translation="MDRLDHIISFIEGDVRSGGFIEPPDRLAGLCDRVFDVIGTYKPS
                     VIVKAGLGRGGLVRAIAESFDAYLVVVEPSLSLIRDFVDGNRGQSWLDRVRCICGDLE
                     SFPVDYYAADLLICTDYLNLF"
     gene            567..1382
                     /locus_tag="PKJ16_18985"
     CDS             567..1382
                     /locus_tag="PKJ16_18985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HNV49131.1"
                     /translation="MPNVKVQYDSWKEDRDIVLEYNLVKLEDATVANILGVGVDNVTR
                     SQAIVKVVNLIESGGVHHIMLMNPYKLYRIRSNNDLRTISGKATMHLPCGAGLSWAAR
                     MLGMPLKERIAPLGFIMELIRFAEIKEYSIFIVGGKPEVAEKAFFNIRKSFPKIRVVG
                     RHGGYFNPEREKSVIEAMRKSEAKIILVGMGFPKEDKWINEIRKDFVNTVFISVGGSI
                     DVISGDIKKAPAFFMERGLEWFYRIITRPWRIGRLAIAGLFCVQVTFKRLFKK"
     gene            1473..1937
                     /locus_tag="PKJ16_18990"
     CDS             1473..1937
                     /locus_tag="PKJ16_18990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49132.1"
                     /translation="MAIRTTAHLHLSTLALIDSARQKTRQRRNDIVVALLTRAMTDPR
                     METVLGRAIRYQDRDRKECWRTIHVSFSEDEADYFTDLRNFFKLSISLIIAITARRYL
                     SEMPNATESRSRVDNYPFQNYVLSRETIDGLIYWKICWGFPHDPQNHFPTPP"
     gene            complement(1982..2200)
                     /locus_tag="PKJ16_18995"
     CDS             complement(1982..2200)
                     /locus_tag="PKJ16_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49133.1"
                     /translation="MSANFVSVIFCRNCYSRYVEISEWEGGRAVLHCRTCGTTEEIAH
                     FTLGRCRVTNTELQNARDSQAKRGKAEK"
     gene            complement(2202..>2530)
                     /locus_tag="PKJ16_19000"
     CDS             complement(2202..>2530)
                     /locus_tag="PKJ16_19000"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV49134.1"
                     /translation="LLPGDPQGLGAARYIRYGTKPLGVAGEEARRVSVALRSGDAFVV
                     MTDGVISLNNRRGQQLGLKRVMDFFSREFSGAEDILDSLLRFADEFTEGLEKREDISI
                     VALVVD"
BASE COUNT          556 a          760 c          734 g          480 t
ORIGIN      
        1 cgaagaggtt gaggtaatcg gtgcagatga gcaggtccgc ggcgtaataa tccaccggga
       61 aggactcgag gtcgccgcag atgcagcgca cccgatccag ccatgattgc ccgcggttgc
      121 cgtcgacgaa atcgcgaatg agcgaaagcg agggctccac cacgacgagg taggcgtcga
      181 acgattccgc aatcgcgcgg acgagcccgc cgcggcccag gccggccttg acgatcaccg
      241 acggcttata ggtcccgatg acgtcgaaga cgcgatcgca caggcccgca aggcgatcgg
      301 gcggctcgat gaatccgccg gagcgaacgt ctccctcgat aaaactgatg atgtgatcga
      361 gtcggtccat gcaccatccc tgccttggct atgcgcgatg cctccggcgc gagccgggat
      421 ggcgagcata cagggcgcag cgcaacgcgc aatcataaat aaatttattg taaaaataat
      481 tgacaccggc gccattttcc ccctaggtac gaattatgtc ctgttgcgac gcgcaacggc
      541 acgcagctat cgggaggagc gcattcatgc ccaacgtgaa agtacagtac gattcctgga
      601 aggaggatcg ggacatcgtc ctcgaataca atctggtgaa gctcgaggac gcgaccgtcg
      661 cgaacatcct tggcgtgggc gtggataatg tcacccgatc gcaggccatc gtgaaggtgg
      721 tgaacctgat cgagtccggc ggcgttcatc acatcatgct catgaacccc tacaagctct
      781 accgcattcg ctccaacaac gacctgcgca ccatctccgg caaggcgacc atgcacctgc
      841 cctgcggggc cggcctcagc tgggcggcgc gcatgctggg catgccgctg aaagagcgca
      901 tcgcgccgct gggcttcatc atggagctga tccgcttcgc ggagatcaag gaatactcga
      961 tcttcatcgt gggaggaaag ccggaggtcg cggagaaggc cttcttcaac atcaggaaat
     1021 cgtttccgaa aatccgcgtc gtggggcgtc atgggggcta cttcaacccg gagcgcgaga
     1081 aatcggtgat cgaggccatg cgcaagtcgg aggcgaagat catcctggtg ggcatgggtt
     1141 ttcccaagga agacaagtgg atcaacgaga tccgcaagga cttcgtgaac accgtgttca
     1201 tcagcgtggg cggcagcatc gacgtgattt ccggcgacat caaaaaggcg ccggcgttct
     1261 tcatggagcg cgggctcgag tggttctatc gcatcatcac ccgcccctgg cgcatcgggc
     1321 ggcttgccat cgcggggctg ttctgcgtgc aggtgacatt caagcgactg ttcaagaagt
     1381 agttttagcg accccggaat acttcgcatt tttttatttc agaatcgcca tcatcgcccg
     1441 taatagaagt attacatcaa tggggtgatg atatggcgat tcgcacgacc gcacacctgc
     1501 atctatccac acttgcgctg atcgattccg cgcgccagaa gacgcgtcaa cgtcgaaatg
     1561 atattgttgt cgcgctgctc acgcgggcga tgaccgaccc gcgcatggag accgtgctcg
     1621 ggcgggcgat ccggtaccag gaccgagacc ggaaggaatg ctggcgtacg attcatgtga
     1681 gtttcagcga ggatgaggcg gactatttta cggatttgag gaattttttc aagctttcga
     1741 tttctttgat tattgcaata accgctcgaa gatacttgag cgaaatgccc aacgccacgg
     1801 aatcgcgatc gagagtggat aactatccat ttcaaaacta tgttctatcg agagaaacga
     1861 tcgacggatt gatatactgg aagatctgct ggggcttccc ccacgaccca caaaaccact
     1921 tccctacgcc cccataaaca accgccgacg ccctgcgtca ctcgagctca aacaaacgcc
     1981 atcacttctc cgccttcccc cgctttgcct ggctgtcgcg cgcgttttgc agctccgtgt
     2041 tcgtgacgcg gcaccgaccc agcgtgaagt gcgcgatctc ctcggtggtc ccgcaagtgc
     2101 ggcagtgcag caccgcccgg ccgccttccc actccgagat ctccacgtag cgggagtagc
     2161 agttgcgaca gaagatcacg gacacgaaat tggcgctcat gttagtccac caccagggct
     2221 acaatcgaga tgtcctcgcg cttctccagc ccctccgtga attcgtcggc gaaccggagg
     2281 agcgaatcca ggatgtcctc cgcgccggag aactcccgcg agaaaaaatc catgacgcgc
     2341 ttgagcccca gctgctgccc gcgccggtta ttgaggctaa tgacgccgtc ggtcatcacc
     2401 acgaacgcat cgccgcttcg cagcgcaacg ctcacccggc gcgcctcctc cccggcgacc
     2461 cccaggggct tggtgccgta gcggatatag cgcgccgcac caagcccctg ggggtcgccg
     2521 gggaggaggc
//
LOCUS       DAORHF010000397         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_49634_length_2524_cov_4.405832, whole genome shotgun sequence.
ACCESSION   DAORHF010000397 DAORHF010000000
VERSION     DAORHF010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49634_length_2524_cov_4.405832"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PKJ16_19005"
     CDS             <1..321
                     /locus_tag="PKJ16_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012162814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HNV49135.1"
                     /translation="VATICIGQAASMGSLLMAAGAAGKRSALPNSRIMIHQPAGGFRG
                     QASDIEIQAKEILKIKQRLNQIYAKHTGKPVEKIEKDMDRDYFMSPEEALEYGLIDKI
                     LERR"
     gene            334..1593
                     /gene="clpX"
                     /locus_tag="PKJ16_19010"
     CDS             334..1593
                     /gene="clpX"
                     /locus_tag="PKJ16_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HNV49136.1"
                     /translation="MSNNKKSKNEKLFCSFCGKSQDVVKKLVAGPSVYICDECIELCN
                     EIVSDEWDSTGPEKNFKDLPKPKEIKGILDQYVVGQEEAKKILSVAVYNHYKRIASNA
                     NLKRDDVELEKSNIVLIGPTGCGKTLLAQTLAKILKVPFAIADATSLTEAGYVGEDVE
                     NILLRLIQNADGDIKKAETGIVYVDEIDKISRKSENPSITRDVSGEGVQQALLKIIEG
                     TVANVPPQGGRKHPHQEFLSINTKNILFICGGAFVGLDKIVQKRMGNKTMGFGADIKS
                     SAEMSGVNPLHQVQPDDMMKYGLIPEFVGRLPIQAVLDNLDRASLIKILTKPRNAIIK
                     QYQKIFSFEDVTLEFAPEAIDEIADTAIKRESGARGLRSVLEKLMLDLMFEVPSKEGV
                     EKIVIGREVVTGAEKPAIIFKKEEKTA"
     gene            1780..>2524
                     /locus_tag="PKJ16_19015"
     CDS             1780..>2524
                     /locus_tag="PKJ16_19015"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HNV49137.1"
                     /translation="MKTPLDFNRKYPLLPLRDVVVFPGMVIPLFVGREKSIGALDAAM
                     KGDRLIVLVTQTDAKVAVPKRSEIFEVGTIAEILQLLKLPDGTIKVLVEGLGRGYINE
                     FIETNKDCLEVTVTNIERPMEVDSEIVALKRAVLEAFEKYIKLNKKVPDEALTTVSNI
                     ENPAHLADVIASHVNLRIELKQMLLDLGNPKDRLEKLVEYINGEIEVMYIEKKIQGKV
                     RKQMEKTQKEYYLNEQMKAIKKELGQGDEE"
BASE COUNT          603 a          699 c          802 g          420 t
ORIGIN      
        1 gtcgccacca tctgcatcgg ccaggcggcc tcgatgggtt cgctgctcat ggcggcgggc
       61 gccgcgggaa agcgttcggc gcttcccaat tcgcgcatca tgatccatca gcctgcggga
      121 gggtttcgcg gacaggcatc cgacatcgag atccaggcga aggagatcct caagatcaag
      181 cagcggctca accagattta cgcgaaacac acgggcaagc ccgtcgagaa gatcgagaag
      241 gacatggacc gggactactt tatgagtccc gaggaggcgc tggagtacgg cctcatcgat
      301 aaaattctgg aacgccgata gcgcgagggg actatgtcga acaataaaaa gagcaagaac
      361 gagaagctgt tctgttcatt ttgcggaaag agccaggacg tggtgaagaa gctcgtggcg
      421 ggtccgtcgg tgtatatctg cgacgagtgc atcgagctgt gcaatgagat cgtttccgac
      481 gagtgggact cgaccggtcc cgagaagaac ttcaaggacc ttcccaagcc caaggagatc
      541 aagggcattc tggaccagta cgtcgtggga caggaggagg ccaagaagat cctttcggtc
      601 gcggtgtaca atcactacaa gcgcatcgcc agcaacgcca acctcaagcg cgacgatgtg
      661 gagctcgaga agagcaatat cgtactcatc gggcccaccg ggtgcgggaa gacgctgctc
      721 gcgcagaccc tggcgaagat cctgaaagtc cccttcgcca tcgccgacgc gacctcgctc
      781 accgaggccg gctatgtggg cgaggacgtg gagaacatcc tcctgcggct catccagaac
      841 gccgacgggg acatcaagaa ggccgagacc gggatcgtgt acgtggacga gatcgacaag
      901 atatcgcgca agtcggagaa cccgtccatc acccgggacg tgtcgggcga gggcgtgcag
      961 caggcgctcc tgaagatcat cgaggggacg gtggccaatg tcccgccgca gggcggacgg
     1021 aagcacccgc accaggagtt cctctccatt aacaccaaga acatcctctt catctgcggc
     1081 ggggccttcg tgggtctcga taagatcgtc cagaagcgga tgggcaacaa gaccatgggc
     1141 ttcggcgcag acatcaaatc gtccgcggag atgtcgggcg tgaacccgct ccaccaggtg
     1201 cagcccgacg acatgatgaa atacggcctc atccccgagt tcgtgggacg gctccccatc
     1261 caggcggtgc tggacaatct tgaccgggcc tcgctcatca agatcctcac caagccgcgc
     1321 aacgccatca tcaagcagta tcagaagata ttctcgttcg aggacgtgac gctggagttc
     1381 gcgcccgagg cgatcgacga gatcgccgac acggccatca agcgggagtc cggcgcgcgg
     1441 ggcctgcgct ccgtgctcga gaagctcatg ctggacctca tgttcgaggt gccgtcgaag
     1501 gagggcgtgg agaagatcgt cattggccgc gaggtggtca ccggcgccga gaagcccgcg
     1561 atcatcttca aaaaggaaga gaaaacagcg taattttttg aaacgtatcg cgctgtttgg
     1621 taatctaact gtcgtaccac tgatgcatgg ccggtcgcga ccgggtcgta cgcgttgatg
     1681 gagcggttcc cggaaccgtg atcgggaagc gcgaaagacc atcgataccg acgcacagac
     1741 cggcgaaatg cagtatgttt ataacctggg caggtgtgca tgaaaacccc gttggatttc
     1801 aatagaaaat acccgctgct cccgcttcgg gacgtggtgg tgtttcccgg aatggtgatc
     1861 ccgctcttcg tcgggaggga gaagtcgatc ggcgcgctgg acgcggccat gaagggcgac
     1921 cgcctcatcg tgctggtcac ccagaccgac gcgaaggtgg cggttcccaa gcgaagcgag
     1981 atattcgagg tgggaaccat cgccgagatc ctgcagctcc tgaagcttcc ggacggcacc
     2041 atcaaggtgc tggtggaggg attggggcgc ggctatatca acgaattcat cgaaacgaac
     2101 aaggactgcc tcgaggtgac cgtcacaaat atcgagcgtc cgatggaggt cgattccgag
     2161 atcgtcgcgc tcaagcgcgc ggtgctggag gccttcgaga agtacatcaa gctcaacaag
     2221 aaggtgccgg acgaggcgct cacgaccgtg agcaatatcg agaacccggc gcacctggcg
     2281 gacgtgatcg cctcgcacgt gaacctgcgc atcgagctca agcagatgct gctcgacctc
     2341 ggcaatccga aggaccggct ggagaagctc gtcgagtaca tcaacggcga gatcgaggtg
     2401 atgtacatcg agaagaagat ccagggcaag gtccgcaagc agatggagaa gacgcagaag
     2461 gaatattacc tgaacgagca gatgaaggcc atcaagaagg agcttggcca gggcgacgag
     2521 gagg
//
LOCUS       DAORHF010000398         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_50007_length_2512_cov_5.538869, whole genome shotgun sequence.
ACCESSION   DAORHF010000398 DAORHF010000000
VERSION     DAORHF010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50007_length_2512_cov_5.538869"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="PKJ16_19020"
     CDS             complement(<1..552)
                     /locus_tag="PKJ16_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49138.1"
                     /translation="MVHHITACFLAALLALPCFTSCKGKLDEAAESFLDRRPTLFRIV
                     SDRALVRSDTDETAPITAISGTVPTHTFGGTYPADGGIVPTGTGIVATGSGIVPTGSG
                     ITPPLGGLTGDNATVPFVANISVSFSFPEIAEKAAKILAPFAAIPFLFTGFTVDRSQI
                     TATADFIARAVAGNGRETDYLDSN"
     gene            complement(590..>2512)
                     /locus_tag="PKJ16_19025"
     CDS             complement(590..>2512)
                     /locus_tag="PKJ16_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HNV49139.1"
                     /translation="IVVKQKDGAVRTISRDAILRIQYTELYMGRVMIEQTDGLSFEGH
                     IVNDDGDAYLVRTDIARPEERSIRKRDVARISRENPDGLRGSYGDGKIRLEWNRPFTR
                     PARYHVYVRGPGEDGFRRIDTTGRTRFTVDDVRADASYAFRVTAEKNDVESIPSNEIS
                     VFTGKLSVDEASSAAPLRLSMDELTRRGAAEAHIFLRDGQIENGTLTAETPAVYRMTR
                     FDGKPVTVPKRSVLRVIYRKMYLGMAYIRLTTGKVLGGFIVDEDRNTVTYRRDLFRPE
                     EKKIPRKDIVFISQSGPTNLKGEAGQTEIALTWDAPYTPVKHYRIFTRTPEAPEYAFA
                     ARSGDNRFTLRKMKSNTEYAVIVTAVGLDDNESFPTNAVRVTTKNIPPEAPTGLTKEK
                     TPDPGSSAFSLRITWNPSRDPDGAVVRYHVYRDSGKGYARIASPTVPEYIVSGLDPGE
                     LHSFYVLAEDDRGALSEKSATADTGTDIEIIVRAEYLQPLGDFANLFGGGYGGTVTVA
                     VREPFVDNLDIGLTTGYWLFTRKRESVKSAFAVPALLSARYRFRLAPWLSLAPSISAG
                     VSYFSVTYEDAVVNESYENIAGGGAVNALVTGGCTAYFHLGGSVSLQVGVEAGAFIET
                     NLYPFAMGTIGIGIVF"
BASE COUNT          424 a          810 c          802 g          476 t
ORIGIN      
        1 attgctgtcc aggtagtcgg tctcgcggcc gtttcccgcc acggcgcgcg cgatgaagtc
       61 cgccgtcgcc gtgatctgcg agcgatccac cgtgaacccc gtaaacaaga aggggatggc
      121 tgcgaagggc gcgagtatct tcgcggcctt ttccgcgatc tccggaaacg agaacgagac
      181 actaatgttc gcgacaaacg gcaccgtcgc attgtcgcca gtcaggccac cgagcggcgg
      241 cgttatcccg gagccagtgg gcacgatgcc gcttccggtc gcgacaatac cggtaccggt
      301 gggaacgata cccccatctg ccggataggt ccccccgaag gtatgcgtcg ggaccgtccc
      361 cgaaatcgcg gtgattggcg cggtttcgtc ggtatcgctg cgcaccagcg cgcggtcgga
      421 cacaatgcga aacagcgtgg ggcggcggtc gaggaacgat tccgcggcct cgtcgagctt
      481 gcccttgcag gacgtgaagc agggtagcgc aaggagggcc gcgaggaagc aagccgttat
      541 gtgatgtacc atatgattgt ttcgtgcgat catatcatca tccccttttt taaaacacga
      601 tccctatgcc gatggttccc atggcgaagg gatacaggtt ggtttcgatg aacgcccccg
      661 cctcgacgcc cacctgcagc gacacggagc cgcccagatg aaaatacgcc gtgcagccgc
      721 cggtcacgag cgcgtttacc gcgccgccgc cggcgatgtt ctcatagctc tcgttcacca
      781 cggcgtcctc gtaggtcacg gaaaaatacg acacccccgc cgagatcgac ggcgcgagcg
      841 acagccacgg ggcgaggcgg aaccggtatc gcgcggagag gagcgcgggg accgcgaacg
      901 cgctcttcac gctctcgcgc ttgcgggtaa agagccagta gcccgtcgtg agcccgatgt
      961 ccaggttgtc gacaaacggc tcgcgcaccg cgacggtcac ggtcccgccg tagccgccgc
     1021 cgaagaggtt cgcgaaatcc cccagcggtt ggaggtactc ggcgcgcacg atgatttcga
     1081 tgtcggtccc cgtgtccgcg gtggcgcttt tttccgagag tgcgccgcga tcgtcctctg
     1141 caagtacgta aaaggaatgg agctcgccgg ggtccagtcc cgaaacgatg tattccggga
     1201 ccgtgggcga ggcgatccgc gcatagccct tcccgctgtc ccgatacacg tgatagcgga
     1261 ccaccgcgcc gtccgggtcg cgcgacgggt tccacgtgat gcgcagggaa aacgcgctcg
     1321 aacccgggtc gggcgttttc tctttggtaa ggccagtcgg ggcctcgggc gggatgttct
     1381 ttgtggtaac gcgcaccgcg ttcgtgggga acgattcgtt gtcgtccagc ccaacggcgg
     1441 tcacgatcac ggcgtattct gtgttgctct tcatcttccg cagggtgaac cggttgtccc
     1501 cgcttcgcgc cgcaaaggcg tactcgggag cctcgggcgt ccgcgtgaat atccggtagt
     1561 gctttactgg cgtgtacggc gcgtcccacg tgagcgcgat ctcggtttgc cccgcctcgc
     1621 ccttcaggtt cgtggggccg ctctgcgata tgaagacgat gtccttccgc ggtatctttt
     1681 tctcctccgg cctgaagagg tcccggcgat aggtgacggt attgcggtcc tcgtccacga
     1741 taaatccgcc aaggaccttc cccgtggtga ggcgtatgta cgccatgccc aggtacatct
     1801 tgcgatagat gacgcgcagc acgctccgct tcgggacggt cacgggcttc ccgtcgaagc
     1861 gcgtcatgcg gtacacggcg ggcgtctcgg cggtcagcgt accgttttcg atctgcccgt
     1921 cccgcaaaaa aatatgcgct tcggcggcgc cccgccgcgt gagctcgtcc atcgagaggc
     1981 gcagcggcgc ggcgctcgag gcctcgtcca cgctcaactt cccggtgaac accgatatct
     2041 cgttggaggg aatggattcc acgtcgttct tctccgccgt gacgcgaaac gcgtacgagg
     2101 cgtccgcgcg gacatcatcg acggtgaacc gggtccggcc cgtcgtgtcg atgcggcgaa
     2161 acccgtcctc gccggggccg cgcacgtaca cgtgatagcg cgcgggcctg gtgaacggcc
     2221 ggttccactc cagccggatc ttgccatcgc cgtagctgcc acgaagcccg tccgggtttt
     2281 cgcgcgagat tcgggcaacg tcccgtttgc ggatgctccg ctcctcgggg cgggcgatgt
     2341 ccgtgcggac caggtaggcg tcgccgtcgt cgttcacgat atgcccctcg aacgacaagc
     2401 cgtcggtctg ctcgatcatc accctgccca tgtacagctc cgtgtactgg atgcgcagga
     2461 tggcgtcgcg cgaaatcgtg cgcaccgcgc cgtccttctg cttcaccacg at
//
LOCUS       DAORHF010000399         2511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_50054_length_2511_cov_4.018322, whole genome shotgun sequence.
ACCESSION   DAORHF010000399 DAORHF010000000
VERSION     DAORHF010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50054_length_2511_cov_4.018322"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PKJ16_19030"
     CDS             <1..669
                     /locus_tag="PKJ16_19030"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HNV49140.1"
                     /translation="QAPEGVMDYIVGDIHGMADKLRGLVQIIEPHLTDDDTIVFIGDY
                     IDRGPSPYEVVEFLVRLADTYNVVFLKGNHEDLFLRYVSGRDPDGVYLFNGGSYTIQS
                     YRAHGGVHRLPARHEEFFNRLRMYHEGSGYIAVHAGLNPDTARLAEQTEHDMLWIREA
                     FYRSSYRWPATVVFGHTPTMQLTGSQEVYVNDEINIIGIDTGAVYGGPLTCLRMPDRI
                     IFQS"
     gene            706..1899
                     /locus_tag="PKJ16_19035"
     CDS             706..1899
                     /locus_tag="PKJ16_19035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HNV49141.1"
                     /translation="MKRFRLRSAAGAVALVAVIAGAAFAVQEYHLSAKVESKRKDGTL
                     TLLFTERPDRSEYNIIDDNAPIGKIAVISVEDRSTGAAPAFRVLASYIVFDTRAEQRV
                     RTGASIGLAVRRIESAYGYARSRSESKLHKQIATEPDGRIMLLVPGGKFLRGSNRGDR
                     DEYPQRIVDVPGFYIDKYEVSNLDFKRFVAAANHKPPRSWPGGAYPPGADTLPVLVTY
                     YEADAYARWAGKRLPTEDEWEKAARGPGVVEVVKGGERRTEPSNPIVYPWGNSFSPER
                     ANCREFWDDARVVSPLKREFSRGLLPVTAFEGKGDSPYGVVNMAGNAMEWTATWYRAY
                     EGNRTPHRRYGAQVKAVRGGAWHHDRFKLRASDREYGGIPSLYTDSLAGFRCARNLRV
                     ADRAR"
     gene            1995..>2511
                     /locus_tag="PKJ16_19040"
     CDS             1995..>2511
                     /locus_tag="PKJ16_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49142.1"
                     /translation="MRFFYLLARRTSYGFAIVFFTVLGGWLGAFFGYYLGFSFLLSTD
                     GTYTSLVLKWFPLAVLVPTFIHYVHFGLLSPIQIPALARSLRLINNNIKGDALRPNIG
                     DDELVALYRSFSDLPTYNAAFAAFYALLVGLLTIGLVYYDIRFLSNITTVDVRLGIRI
                     ITVADIVVVVLY"
BASE COUNT          432 a          787 c          802 g          490 t
ORIGIN      
        1 caagctcctg agggcgtgat ggactacatc gtcggggaca tccacggcat ggcggacaag
       61 ctccggggcc tcgttcagat catcgagccg cacctcaccg atgacgacac cattgtcttc
      121 atcggcgatt acatcgaccg ggggccgtcg ccctacgagg tcgtggagtt tctggtgcga
      181 ttggccgaca catataacgt cgtgtttctc aaggggaatc atgaggacct cttcctgcgg
      241 tatgtgagcg ggcgtgaccc ggacggggtg tacctgttca acggcgggtc gtacaccatc
      301 cagagttaca gggcgcacgg gggcgtgcat cggctccccg cccggcacga ggagttcttc
      361 aaccgcctgc ggatgtatca tgaggggtcc ggctatatcg ctgtccatgc ggggcttaac
      421 ccggatacgg cgcgtcttgc cgaacagacc gagcacgaca tgctgtggat tcgcgaggcg
      481 ttttaccgtt cgagctatcg gtggcccgcg acggtcgtgt tcggccatac gcccacgatg
      541 cagctcaccg ggtctcagga ggtctatgtc aacgacgaga tcaatatcat cggcatcgat
      601 accggggccg tatacggcgg tccgctcacc tgcctgcgca tgccggaccg catcatcttc
      661 caatcatagg gctgtgggcc gcgcggtacg tgccgggagg tcgcgatgaa gcgttttcgg
      721 ttgcgaagtg ccgccggggc cgtcgcgctt gtcgcggtga tcgccggtgc ggccttcgcg
      781 gtgcaggaat accatctgag cgcgaaggtc gaatcgaagc gcaaggacgg gaccctgacg
      841 ctcttgttca cggagcgccc ggaccgatcc gagtacaaca tcatcgatga caatgcgccc
      901 atcggcaaga tcgccgtcat aagcgtcgaa gaccggagca cgggcgccgc gcccgcattt
      961 cgggtcctgg cgagctatat cgttttcgat acgcgcgccg aacagcgtgt ccgcacgggc
     1021 gcgtccatcg gactggccgt tcggcgcatc gagtccgcat acggctatgc gcgctcgcgc
     1081 tccgaatcga aactgcacaa gcagattgcc accgagcccg acggcaggat catgctgctc
     1141 gtcccgggtg gaaagttttt gcgcggctcg aaccgcggag accgggacga gtacccgcaa
     1201 cggatcgtcg acgtgcccgg gttttacatc gacaaatacg aggtctcgaa cctcgatttc
     1261 aagcgcttcg tggcggccgc gaaccataaa ccgccgcgct cctggcccgg cggggcgtat
     1321 cctcccggtg cggacaccct gccggtcctg gtgacgtatt acgaggcaga cgcctatgcg
     1381 cgctgggcgg gcaagcgcct tcccaccgag gacgaatggg agaaggccgc ccgcggcccc
     1441 ggggtggtcg aggtggtcaa gggcggcgag cggcgcaccg agcccagcaa tcccatcgtc
     1501 tatccgtggg ggaactcctt ctccccggag cgggccaatt gccgggagtt ctgggacgat
     1561 gcccgcgtgg ttagtccgct caagcgcgag ttttcgcggg ggctccttcc ggttacggcc
     1621 ttcgagggga agggtgattc gccgtacggc gtggtgaaca tggccggaaa cgcaatggag
     1681 tggacggcga cctggtaccg ggcctatgaa ggcaaccgca ccccgcatcg gcgctatggc
     1741 gcccaggtga aggcggtgcg ggggggcgcc tggcatcacg accgcttcaa gctccgggcg
     1801 agcgaccggg agtacggcgg cattcccagc ctgtatacgg attcgctcgc ggggttccgc
     1861 tgcgcccgga acctccgggt cgccgaccgc gcgcggtagc gcgcggtcgg atcatactcc
     1921 attaaccggt aaattattgc gccgaaatga cgataagtat accagggagc atatcttcca
     1981 tctaggggac cgtcatgagg tttttttatc tgctggcgcg ccggaccagc tatgggttcg
     2041 ccatcgtgtt cttcaccgtg ctggggggat ggctgggggc cttcttcggc tactatctcg
     2101 gcttttcgtt tctcctgtcc accgacggga cctacacatc gctggtgctc aaatggttcc
     2161 cgctggccgt gctggtcccc acgttcatcc attatgtgca cttcggcctc ttaagcccca
     2221 tccagatccc ggcgctcgcg cgctccctgc ggctcatcaa caataacatc aagggcgatg
     2281 cgctccggcc gaacatcggc gatgacgagc tcgtggcgct ctatcggtcc ttctccgacc
     2341 tgccgaccta caatgcggcc ttcgcggcct tctacgccct gctggttggc ctgctcacca
     2401 tcgggctggt gtattacgat atccggttcc tgagcaacat caccaccgtc gacgtgcgtc
     2461 tgggcatccg catcatcacc gtggccgata tcgtcgtggt ggtgctctac g
//
LOCUS       DAORHF010000400         2495 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_50576_length_2495_cov_4.721311, whole genome shotgun sequence.
ACCESSION   DAORHF010000400 DAORHF010000000
VERSION     DAORHF010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50576_length_2495_cov_4.721311"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PKJ16_19045"
     CDS             complement(<1..616)
                     /locus_tag="PKJ16_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HNV49143.1"
                     /translation="MRKSFLLSSIAAIATLAAAMTYSSIVSADSAKIIIKGSTTVLPI
                     TQKAIEAYPESVAKKVAISVDGSGSGNGIKAIMDGTCDIANSSRAMKKEELAKAKEKK
                     LRIKEIVVAWDMIVPVVHPKNPVASLTKDQLKGIYEGTIKNWKEVGGPDMRIVVISRD
                     TSSGTYEFWYEDVLKKTNVRKDALLQASNGAVVATVANNEKAIGY"
     gene            904..2025
                     /locus_tag="PKJ16_19050"
     CDS             904..2025
                     /locus_tag="PKJ16_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49144.1"
                     /translation="MMKRKSLFFSAVLLLAAIVGVMSVTDAFAVVRKQNQRTMEKLSL
                     NVVPAKDVTLFLKGANRYEHWNRADTEWNKGENAYKNQAGGTVEWEYEVGGSYNLKLS
                     DDFSLGLGAYWYQEVYPSDQYSGPASDKLGNKAGDPPYLDSINFDIAPKYKLGDASIY
                     GRVRFVNEVFWGNTEVWTKRSYATSAVNDLRGKRVGYDKSEEYGWAMTTRILLGADYK
                     VTKKFTVFGDFEVRYGTIEDNETKFVPGLDEKVIRTGRGAGYNPKGPDGNSDTDPDVS
                     TGIAHGKYFKTHGLWRYTWQVGMKLQLTDALGMTLSYFQETNLNPYHNPNDPVVQNKK
                     TGAISDYRYTWNGVPNTQQIVGQNNYLYLELAYKLDLTK"
     gene            complement(2305..>2495)
                     /locus_tag="PKJ16_19055"
     CDS             complement(2305..>2495)
                     /locus_tag="PKJ16_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49145.1"
                     /translation="FLDEVRPELAKTTVTYFGIEQGSPFIFLTVFAERTGKRSKHKFG
                     IMEMNDGSLAIGSYEPAR"
BASE COUNT          547 a          653 c          712 g          583 t
ORIGIN      
        1 tatagccgat ggccttctca ttgttggcca cggtggcgac caccgcgccg ttcgaggcct
       61 gcagaagcgc gtccttgcgg acattggtct tcttgagcac gtcctcgtac cagaactcgt
      121 aggtgccgga gctcgtatcg cgcgaaatga ccacgatgcg catgtccggg ccccccacct
      181 ctttccagtt tttgatggtg ccttcgtaga tgcctttaag ctggtccttg gtgaggctcg
      241 caaccgggtt cttcgggtga acgaccggga cgatcatgtc ccaggcgacc acgatctcct
      301 tgatgcggag cttcttttcc ttcgccttgg cgagctcttc cttcttcatt gcgcgcgacg
      361 agttcgcgat gtcgcaggtg ccgtccatga tcgccttgat gccgttcccg gatccgctgc
      421 cgtccaccga gatggcgacc ttcttcgcga ccgactccgg atacgcctcg atcgcctttt
      481 gggtgatcgg gagcacggtg gtggagcctt taatgatgat cttcgcgctg tcggcgctca
      541 cgatcgacga gtacgtcatg gctgccgcga gtgtcgcgat cgcggcgatg ctgctgagta
      601 aaaagctttt acgcatgggc tgtatgcctc ctgagtgatg atgaatcaaa tgtgcttggt
      661 cggaaaatgg aaaccgcggg tgaatgaacg ataatggttt tgtaaatatt ttgttaacct
      721 ggggggtttt tttatcggga cgccccgtgt cgcgccaaga aataattaac ggaaaataaa
      781 catttcactg atgattactt aatcctcgcc gcgcttattg ttttagaaaa ataacggcgg
      841 atcggagtga agcgattcca ttcgttcgtt attcatcaat catcaaacaa ggagacaagt
      901 agaatgatga aacgcaagtc gctctttttt tcggctgtgc ttctgttggc cgccatcgtg
      961 ggcgtcatgt cggtgacaga cgccttcgca gtcgtgcgca agcagaacca gcgcaccatg
     1021 gaaaagttgt cgctcaacgt agtccccgcc aaggacgtga cgctctttct caagggagcc
     1081 aatcggtacg agcactggaa ccgcgcggac accgaatgga acaaggggga aaatgcctac
     1141 aagaaccagg ccggcggaac ggtcgagtgg gagtacgagg tcggcggaag ctacaacttg
     1201 aagctgtccg atgatttctc gctgggcctt ggtgcgtatt ggtatcagga agtctatccc
     1261 agtgatcagt atagcgggcc cgcgtccgac aagctgggta ataaagccgg cgatccgccg
     1321 tatctggatt caatcaattt tgacatcgcg cccaagtata aacttggcga tgcgagtatc
     1381 tatgggagag ttcgtttcgt gaacgaagtc ttctggggta atactgaggt gtggactaaa
     1441 agaagttatg ctacttcagc ggtaaatgac ctgaggggta agcgtgtcgg gtacgacaag
     1501 agcgaggagt atggctgggc catgacgacc cggatccttc ttggcgcgga ttacaaggtc
     1561 accaagaagt tcacggtctt cggcgatttc gaggtccgct acgggaccat cgaagacaat
     1621 gagaccaagt ttgttcccgg actcgacgag aaggtcatcc gtaccggacg gggcgccggg
     1681 tataatccga agggcccgga cggaaacagc gataccgacc ccgacgtttc caccggcatt
     1741 gcgcacggga agtatttcaa gacgcacggc ctctggcgct acacctggca ggtcggcatg
     1801 aagctgcagc tgaccgacgc gctcggcatg acgctctcgt acttccagga gacgaacctt
     1861 aatccgtatc acaatccgaa cgacccggtt gttcaaaaca aaaagaccgg cgcgattagc
     1921 gattatcgat acacgtggaa cggcgtcccg aacacgcagc agatcgtagg acagaacaat
     1981 tatctctatc tcgaacttgc ctataagctc gatctcacca agtagccgaa aactttcgtt
     2041 gtttcttcat gtgcatgggc tcccgtatgg gggcccgttt tttcacatat gaaaagtgtg
     2101 aactcttcgc aaggcattca ttcagtgcgg tgttgcatca gattgcttcg cttcgctcgc
     2161 aatgacggtg agattggcat cgggacggtt gaattcaatg cgaagcctgt cgttcgaccg
     2221 tcgtcatgag cgctcgcaat acgcctatac gatccgacct gggagggtgt ttttgaattg
     2281 cggaaaccgg gagctgggtg tcgcttaccg ggccggttcg tagcttccga tggccaggct
     2341 gccgtcgttc atctccatga tgccgaactt atgcttgctg cgcttgcccg tgcgctcggc
     2401 gaacaccgtc aggaaaatga agggagaacc ctgctcgatc ccgaaatagg tcacggtggt
     2461 cttcgccagt tccggccgca cctcgtcgag gaaca
//
LOCUS       DAORHF010000401         2490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_50727_length_2490_cov_4.395483, whole genome shotgun sequence.
ACCESSION   DAORHF010000401 DAORHF010000000
VERSION     DAORHF010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50727_length_2490_cov_4.395483"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PKJ16_19060"
     CDS             complement(<1..1214)
                     /locus_tag="PKJ16_19060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49146.1"
                     /translation="MDSRQQGLSSKLKIAAIDDDFFIRGLLRSFLEEDYDFRSASCWM
                     EFKSLTDDFHPDVLFLDLYLPDGNGIEICRDLKKQKRFENLYIMMLTGSDDKETIIDA
                     YSAGADDFIRKPFVTYEVKSKIANLERLTRYQNAILNEYRHQSVLNRKLFNLSRIIKD
                     HLLTADRDELIAAIDRFNGIMDIAALEVVIDDGGRTETTLSRRFDEKFVPVPADSLSR
                     KLAAFRDREDFMHFCRIQTAGRREVFCCITPLIFNLRSRAYIIIQKRAPFEMEDKKIL
                     SLIVDFFNIMLDRIDIQSTLAKQNNRYRREITNARKIQVALLPEFKELTKFDIAYTFL
                     PAEDISGDFFDGFYLDERTYQIIICDVSGHGVASSYIGSQMRTLFRTFSTPGTPPAEI
                     LRSMQAQITAER"
     gene            1445..2035
                     /locus_tag="PKJ16_19065"
     CDS             1445..2035
                     /locus_tag="PKJ16_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HNV49147.1"
                     /translation="MTSTAKKFDKVYTYADYLGWPHNERWEILGGVAYGMSPAPDRRH
                     QEISGALHNAFYSFLKGRTCRVYAAPFDVRLPESGTSDDSITTVVQPDLVVVCDPAKL
                     DKRGALGPPDLVVEIVSPTSAARDMKEKVHLYEKHGITEYWIVHPEEAIVMVYRHVDG
                     AYGRPDVFAGDDTIEVGVLPGLTIAINEIFPQSQSE"
     gene            2184..2258
                     /locus_tag="PKJ16_19070"
     tRNA            2184..2258
                     /locus_tag="PKJ16_19070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2219..2221,aa:Val,seq:gac)
BASE COUNT          521 a          672 c          740 g          557 t
ORIGIN      
        1 tcgcgctcgg cggtgatctg cgcctgcatc gagcgcagga tctcggcggg cggcgttccg
       61 ggcgtcgaga acgttcggaa gagcgtccgc atctggctgc cgatatacga tgacgccacg
      121 ccgtgccccg atacgtcgca gatgatgatc tggtacgtcc gctcgtcgag gtagaagccg
      181 tcgaagaaat cgcccgatat gtcctcggcg ggaaggaacg tgtaggcgat gtcgaacttc
      241 gttaactcct taaactcggg caggagcgcc acctggatct tccgcgcatt ggtgatctcg
      301 cgacggtagc ggttgttctg cttggccagc gtcgattgga tgtcgatgcg gtccagcatg
      361 atgttgaaaa agtccacgat gagcgagagg atcttcttgt cctccatttc gaagggcgcg
      421 cgcttctgga tgatgatgta ggcgcggctg cgcagattga agatgagcgg cgtaatgcag
      481 cagaagacct cgcgcctgcc cgccgtctga atccggcaga agtgcatgaa atcctcccgg
      541 tccctgaagg ccgcgagctt ccgcgagagc gaatcggccg gaacgggaac gaacttctcg
      601 tcgaaacgcc gggaaagcgt cgtttcggtg cggccgccgt cgtcgataac cacctccagc
      661 gcggcgatgt ccatgatccc gttgaagcgg tcgattgcgg cgatgagctc atcgcggtcg
      721 gcggtcagga ggtggtcctt gatgatgcgg ctcaggttga acagctttcg gttgagcacc
      781 gactggtgcc ggtactcgtt caggatggcg ttctggtagc gggtgagccg ttcgaggttc
      841 gcgatcttgc tcttcacctc gtaggtcacg aagggcttgc ggataaagtc atcggcgccc
      901 gccgagtagg cgtcgatgat ggtctcctta tcgtcggagc cggtgagcat catgatgtag
      961 aggttctcga agcgcttctg ttttttgagg tcgcggcaga tctcgatgcc gttcccgtcg
     1021 gggagataga ggtccaggaa cagcacatcc gggtggaagt cgtcggtgag gctcttgaac
     1081 tccatccagc acgaggcgct ccgaaagtcg tagtcctcct cgaggaaact gcgcaacagc
     1141 ccgcgtatga aaaaatcgtc gtcaatggcg gcgattttca gcttgctgga taatccctgt
     1201 tgtctgctgt ccacggttcc cgcttgctcg catcgatctt tctctagttc agatagatac
     1261 cgcgcctgca gcaatatttc aagcacaatt tgcatagcgg cgatactgca gtattggcgt
     1321 gatagtgcat cctggcgggt gaatacgtga tgaatcacaa tcgcaaaaaa attgacaaat
     1381 accagggaat aatggtagga tagttttagc ttgggttcat cgtgccaaac cgataggatt
     1441 ttttgtgaca tcgactgcga aaaaattcga taaagtgtat acctacgccg attatcttgg
     1501 atggccgcac aacgagcggt gggagatact cgggggagtc gcctacggga tgtcgcccgc
     1561 gccagacagg agacatcagg aaatatcggg cgcattgcat aacgcatttt attcgttctt
     1621 aaagggccgc acctgcaggg tgtatgccgc gcccttcgat gtgcggctgc ccgaatcggg
     1681 aacgtccgat gattcgatta cgaccgtagt gcagccggac ctggttgttg tgtgcgatcc
     1741 cgcgaagctg gacaaacgcg gggcgctcgg cccgccggac ctcgttgtcg agatcgtttc
     1801 tccgacttcc gccgcgcggg acatgaaaga gaaggtgcac ctgtacgaaa agcacggcat
     1861 taccgagtac tggattgtgc accccgagga ggcgatcgtc atggtgtacc ggcatgtcga
     1921 tggcgcgtat ggccgcccgg acgtgtttgc cggtgacgac accatcgagg tcggagttct
     1981 cccgggcctc acgatcgcga taaacgagat ttttccccaa tcgcagtctg aataaaaccc
     2041 agttggaatt tgcaaaccgg caacaaggcc atagtgcgtt tatgggtttc ttgctgtgta
     2101 ccgataacta tcggacctga tccacatcac ccggaaaaaa agattgacaa tggggcggga
     2161 atggcggttt ggtctttgca catgggcgat tagctcagtt tggttagagc gcctgctcga
     2221 cacgcaggag gtcactggtt cgactccagt atcgcccaca ggaagcgcgt ccatctgccc
     2281 cggatgcgct tttttcgtct caagactggt attcccccga ctattgccac aacaccgata
     2341 actatcggcg ccctgcagcg cgtccatcgg attctgtgac gtactaccga taactatcgg
     2401 ccggcaatca ccatgggctc catgcgcgcc gcgacaatcc accgcccccg catcaccatc
     2461 atgcaaatat tttgttgact atttccccca
//
LOCUS       DAORHF010000402         2479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_51110_length_2479_cov_4.111386, whole genome shotgun sequence.
ACCESSION   DAORHF010000402 DAORHF010000000
VERSION     DAORHF010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51110_length_2479_cov_4.111386"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="PKJ16_19075"
     CDS             complement(<1..2264)
                     /locus_tag="PKJ16_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV49148.1"
                     /translation="MRYFLEKRITTYMVFAGLFLFGVIGLSKLPISLMPYTGYPGISI
                     IIEYPGISPEKIETIITKPVEKIVKTVPGIEKIDSVSEDGKARINVNFQMDADVKIAA
                     LKVREKIGLIRGSFPRAVQEPVVIRYDPSDKPIIIATIARKGVSLEEMREIAERKVKP
                     QLQRIEGISEINIAGGRQREIHIDLNQGRFEGRSLSFEKLFTTVQSGNVSLPGGLLAY
                     GKREYVVYTPSRFKSIGEIADTAVLQTAQGSLVRLGDIGQVLDSYRDQEDIARLDGKE
                     NVTIYIQKAGDANILELCNSIVRLFSKLTEYEINVVYNQGRYIRAAITNVVSSCIWGV
                     LIVIMVIYIFFRKLDYVITIGLTIPYTIVVVFACMYFAGVNIDIMSLSGVALGGGMIV
                     DTSIIVTESIFSSRRINTETILGGIGYVRNAVVSSTVTTVIVFLPLVFGDILTQRMYG
                     GMAFTITLALLVSLVVALLLVPAVYQELAIRAPRSKSSISRRSAVFDRLRPLLDAFFA
                     QADSFERTMHQKYEKALGYVFGHPRTVLGVVAVMLAIATGSLFFIKSEFIDPMGTGEF
                     YVYIEFPTGSTLEFANEASRKAEDHVKGLKVTEKISTKVEKMRATLSITLLEEVSSTK
                     KRERIKARIRQDLNNLMTPYGGFAFLSEADEAASREFTITFLGDENETLRSIAKTAAG
                     KIGAIKGIEECVLRFREGRPSYCLYLDREKANLSMLSARDVAGFFHNAVFGPVVTKFV
                     AEDREVDVRVRYLK"
     gene            complement(2272..>2479)
                     /locus_tag="PKJ16_19080"
     CDS             complement(2272..>2479)
                     /locus_tag="PKJ16_19080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HNV49149.1"
                     /translation="KMRLKPILMTTATAVLGMLPIAIGIGEGAELQQPMAIAVIGGLT
                     VSTFLTLVFIPTVYALTHAESTKS"
BASE COUNT          560 a          730 c          665 g          524 t
ORIGIN      
        1 tccttgagat agcggacccg gacgtcgacc tcgcgatcct cggcgacaaa tttggtcacc
       61 accggcccaa agaccgcgtt atgaaagaaa cccgcgacgt ctcgcgcgga aagcatcgag
      121 aggttcgcct tctcgcgatc aagatacagg cagtacgacg ggcgaccctc ccgaaagcgc
      181 agcacgcact cctcgatccc cttgatggcg ccaatcttcc cggcggccgt ctttgcgatc
      241 gagcgaagcg tttcgttctc gtcgcccaga aaggtaatgg taaattctcg ggaagcggcc
      301 tcatcggcct ctgagaggaa ggcaaatcca ccgtacggcg tcatgagatt attcagatcc
      361 tgcctgatcc gcgccttgat tcgctcacgt tttttggtgg acgacacctc ctcgagaagg
      421 gtgatcgaca gcgttgcccg catcttctcc accttcgtgg aaatcttctc ggtcaccttg
      481 agccccttca cgtggtcctc ggcctttcgg gacgcctcgt tggcgaactc aagggtcgag
      541 ccggtcggga actcgatata gacatagaat tccccggtgc ccatcggatc gataaactcg
      601 ctcttaatga agaaaagcga ccctgtcgcg atggcaagca taacggccac caccccaagc
      661 accgtacgcg gatgcccaaa tacgtatccc agcgccttct cgtatttttg atgcatggtg
      721 cgctcgaagg aatccgcctg tgcgaaaaac gcgtcaagga gaggccgcag ccggtcgaac
      781 acggccgacc ggcgggatat cgacgatttc gaacgcggcg cacggatcgc cagctcctga
      841 tatactgcgg gaaccagcaa cagggcaacc acaagtgaga cgagtagcgc caaggtgatg
      901 gtaaaagcca tgccgccata catgcgctgc gtcaggatat ccccgaatac caacggcaaa
      961 aacacaatga cggtcgtgac tgtcgaagag acgaccgcgt tgcgcacata gccaatgccg
     1021 ccaagaatcg tctcagtgtt gatccttcgg ctgctgaaaa tcgactcggt cacgatgatg
     1081 ctggtatcga caatcatgcc gccccccagc gccaccccgc tgagactcat aatgtcgatg
     1141 ttgacgccgg cgaagtacat gcaggcaaaa acgacgacga tcgtataggg aatggtgaga
     1201 ccgatggtta tcacgtagtc cagcttccgg aaaaagatat agatgaccat gataacgatc
     1261 agaacgcccc atatgcacga cgatacgaca ttggtgatcg cggcacggat atagcgtccc
     1321 tggttgtaca ccacgttaat ctcgtattcc gtgagcttcg agaaaagacg cacgatgcta
     1381 ttgcacaact cgagaatatt cgcatcgccc gctttctgta tgtagatggt tacattctcc
     1441 ttgccgtcaa gccgggcgat atcctcctgg tcgcggtatg aatcaagcac ctggccaata
     1501 tccccaagcc gcaccagcga gccctgcgcg gtctggagaa ccgctgtgtc ggctatctct
     1561 ccgatcgatt tgaatcggct tggcgtgtac acgacgtatt cccgttttcc atacgccaac
     1621 aggccgcccg gcagcgagac attgcccgat tgaacggtgg taaaaagctt ctcgaacgaa
     1681 agcgagcgcc cttcaaagcg cccctgattc agatcgatat ggatctcccg ctggcgaccc
     1741 cccgcgatgt ttatctcgga aatcccctcg attcgctgga gctgcggctt taccttacgt
     1801 tcagcgatct cccgcatttc ctcgagcgaa acgcccttac gggcgatcgt cgcgatgatg
     1861 atcggtttgt ccgatgggtc atatcgaatg actaccggct cctgcacagc tcgcgggaaa
     1921 ctgccccgga tcaggccgat cttctcccgc accttcagcg cggcaatttt aacatcggca
     1981 tccatctgga agttgacatt gatcctcgcc ttcccgtcct ccgaaaccga gtcgatcttt
     2041 tcgatgccag gaacggtttt tactatcttc tcgaccggtt tggtgatgat cgtctcgatc
     2101 ttctccgggg agatgccggg gtactcaatg atgatggaaa tgcccggata gccggtatac
     2161 ggcatcagag aaatcggcag cttgctcaga ccgatgaccc cgaacaggaa gagcccggcg
     2221 aagaccatgt aggtggttat gcgtttttcc aggaagtatc gcatgccggg ttcacgattt
     2281 cgtggattcg gcgtgtgtga gcgcatagac ggtgggaatg aacaccagcg tgaggaaggt
     2341 cgataccgtc aggccgccga ttaccgctat cgccatcggc tgttgcaact ccgcaccttc
     2401 gccaatgccg atggcgatgg gcagcatgcc gagcacggcg gttgcggttg tcatcaggat
     2461 cggtttcagc cgcatcttc
//
LOCUS       DAORHF010000403         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_51891_length_2455_cov_4.933750, whole genome shotgun sequence.
ACCESSION   DAORHF010000403 DAORHF010000000
VERSION     DAORHF010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51891_length_2455_cov_4.933750"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(259..1977)
                     /locus_tag="PKJ16_19085"
     CDS             complement(259..1977)
                     /locus_tag="PKJ16_19085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HNV49150.1"
                     /translation="MGVTLVPKYDGPDGALHNFDLEQVRRLFIMPDSPDKFLEFGHEL
                     LGLIHKFFKEKGGIHSEIPLTRLARIFSDIEVPREPHLLKNVLLEIKDNVIAHSVKVG
                     SPYYIGHMTSAIPYFMILLEMIIAALNQNQVKIETAKASSYVERELLAWIHRVVFDRD
                     PRFYRRFIQDPKVALGNVTVDGTVANLTALLVARNRAFPAKGRFPGIRAAGVYEAFRF
                     YGCRRAVILVSRRGHYSITKVARIIGLGHDSVVQVPVDSDNRIDIGELKRICKEIAEE
                     NARTDEKVRIISLVGIAGTTETGNIDDLHELARVAEINKTSFHVDAAWGGGVMLVDEY
                     RHLFKGIELADSVTFDSHKLLYSPLSMGMVLFRDEKALANIKHTSDYIIRADSVDQGR
                     FTVEGSRPFACLKPWATIKILGSVGFKLLFENAFALTSLLRGIIERHCDMELMNNPEL
                     FIVNYRFAPKKVRRRLAQLMAKINSGESRKEKYLRKKVIEINNLLNELNIELHRAIRE
                     EDNSFVSRTMLESTRYRPQRVVVLRAVVINPLTTPEILKEIIEEHHKLGVCIYKRDFE
                     SRLEKI"
     gene            2255..>2455
                     /locus_tag="PKJ16_19090"
     CDS             2255..>2455
                     /locus_tag="PKJ16_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49151.1"
                     /translation="MTPSPRTPIIYCAAIAALALAVRLLGYDELLSVESLVRHRAALM
                     DLVLRDYALSVALFAVVFIATVS"
BASE COUNT          452 a          750 c          776 g          477 t
ORIGIN      
        1 ggtcgcggcg accgcgtcca ttccgaccgg cgccacgatg cggcgtcgga cgggcccggg
       61 gtgccggcgt aatacagcgc gccgccgctt tcggcgatgc acgacacgcg gagatttatc
      121 tttaccggtt ccggcgcgcc cgcagtgcgg acatataccg cgcccccatc aaaaaaaaac
      181 ctgccggcgg aaacgtctcc ggacaggcag ggtaaaaaaa acagactcag gagaagaaaa
      241 aatggcatgc cccgtcggtt aaatcttctc aaggcggctc tcgaagtccc tcttgtagat
      301 gcacacgccg agcttgtggt gctcctcaat aatttccttg aggatctccg gcgtggtgag
      361 ggggttgatg acgaccgcgc gcagcaccac gacccgctgc gggcggtacc gcgtcgattc
      421 cagcatggtg cgcgagacga agctgttgtc ctcctcgcgg atggctcggt gcaattcgat
      481 attgagctcg ttgagcaggt tgttgatctc gatgaccttt ttgcgcagat acttttcctt
      541 gcgcgattcg ccgctgttga tcttcgccat gagctgggcg aggcggcgcc gcaccttctt
      601 gggggcgaac cggtagttca cgataaagag ctcgggattg ttcatgagct ccatgtcgca
      661 gtgccgctcg atgatgcccc gcagaagcga ggtgagcgcg aaggcgttct cgaagaggag
      721 cttgaatccc acggacccca ggatcttgat ggtggcccag ggcttgagac aggcgaacgg
      781 gcgcgagccc tccaccgtga agcgcccctg gtccacggag tcggcgcgga tgatatagtc
      841 cgaggtgtgc ttgatgttcg ccagcgcctt ctcgtcccgg aacaacacca tacccatcga
      901 cagcggcgag tacaggagct tgtgcgaatc gaaggtgacc gagtccgcga gctcgatccc
      961 cttgaataga tggcggtact cgtccaccag catgaccccg ccgccccacg ccgcgtccac
     1021 gtggaacgac gtcttgttga tctctgcgac acgggcgagc tcgtgcagat cgtcgatatt
     1081 gccggtctcg gtggtccccg cgatccccac cagggagatg atgcggacct tctcgtcggt
     1141 gcgggcgttc tcctcggcga tctccttgca gatccgcttg agctcgccga tgtcgatgcg
     1201 gttgtcggaa tcgaccggca cctggaccac gctgtcgtgt cccaggccga tgatgcgcgc
     1261 gaccttcgtg atcgaatagt gcccgcgccg ggacaccagg atcaccgcgc gccggcagcc
     1321 gtaaaagcgg aaggcctcgt acacgccggc ggcccggatg cccgggaagc ggcccttcgc
     1381 ggggaaggcg cgattacgcg ccaccaggag cgccgtgagg ttcgcgacgg tcccgtccac
     1441 cgtgacattg cccagcgcaa ccttgggatc ctggatgaag cgccggtaga agcgcgggtc
     1501 gcggtcgaac accacgcgat ggatccaggc gagcagttcg cgctccacgt acgacgaggc
     1561 cttggcggtc tcgatcttca cctggttctg gttgagcgcg gcgatgatca tctccagcag
     1621 gatcatgaaa tacgggatcg cgctggtcat gtggccgatg taataggggc tgcccacctt
     1681 cacggagtgg gcgatcacat tgtccttgat ctccaggagg acgttcttca gcaaatgggg
     1741 ctcgcgcggc acctcgatgt cggagaagat tcgcgcgagg cgcgtgagcg ggatctcgct
     1801 gtggatgccg cccttctctt taaagaactt gtggatgaga ccgaggagct cgtggccgaa
     1861 ctcgaggaac ttgtccggcg agtcgggcat gatgaacagg cggcgcacct gctccaggtc
     1921 gaaattgtgg agcgcgccgt cgggtccgtc gtatttcggt accagtgtaa cgcccataga
     1981 acggggaccc tctgcgacaa gattagcgcg cacgccgccg tggatgcgaa ccggacgttc
     2041 gcataaaaca atgtgccggt gcggccgatt tttttcaagt aaaaatgaca taaatccttg
     2101 atatattatt tacagcaatg cgcattgtgt cgatattttt cgcgaaaaag acgcgccgga
     2161 tcgcgcgcga acgcgccatg cgacataacc gcgctcgcgt tatccgaaac catgcgccgc
     2221 cgcgaccgcg cgccggcgct cacccgaggt gcgcatgacg ccgtcgcccc gcacgccgat
     2281 catctattgc gccgcgatcg cggcgctggc gctcgccgtc cgtctgctgg gctacgacga
     2341 gctcctgtcg gtcgagtccc tcgtccgtca ccgggcggcg ctcatggacc tcgttttgcg
     2401 ggactatgcg ctctcggtcg cgctgttcgc ggtcgtcttc atcgcgacgg tgtcg
//
LOCUS       DAORHF010000404         2427 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_52836_length_2427_cov_5.381113, whole genome shotgun sequence.
ACCESSION   DAORHF010000404 DAORHF010000000
VERSION     DAORHF010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52836_length_2427_cov_5.381113"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..1106
                     /locus_tag="PKJ16_19095"
     CDS             330..1106
                     /locus_tag="PKJ16_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49152.1"
                     /translation="MDKEEKSRQMVKSIKMLESVYKTTTSAAQRSRVKKDVETLRARL
                     KELYPEANIKELEEAIYSDMLTLKSNGTGIDEDLEFLRGIEVVEYSPYKDDAEINTAA
                     SILKHFEERIWGVITDQHTKLDFSNSGERDTLYRKLDQCDRALKMFLQTVEDIDKAKS
                     NDYASQLQMMRVKQGRVFQMELNEFLKKARDFVVNLVSDAEFGGTMILNPDDPIAYAE
                     YETYRMFENVKVIDALRFMKNFLEETQKVIRMPDLGKMNK"
     gene            1222..1647
                     /locus_tag="PKJ16_19100"
     CDS             1222..1647
                     /locus_tag="PKJ16_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV49153.1"
                     /translation="MAITKSGGDIADNVIGENSYFTGKFIINGSLRIDGRFEGKYLQA
                     EQLYVGPNGKVRTNINAVSLIIEGLIIGNINASNRVLLMPTAKILGDIKTPELIIQNG
                     VILEGRCTIANDLRSSAKDLIEVEYDKNKIIPEEFIARA"
     gene            1634..>2427
                     /locus_tag="PKJ16_19105"
     CDS             1634..>2427
                     /locus_tag="PKJ16_19105"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HNV49154.1"
                     /translation="MPARDIVVGVTAGDPAGIGPEVACRALMERYDGIVPILICREEV
                     VASLGILGRDRYRVARSVEEIADPSDRDRIALVSPESDAPVPRAGAGTAATGAESLGY
                     VDTAIELWRAGAIDAIVTGPVSKALIEQSGHPFTGHTEYIAAAINETTPYMLMYSDEL
                     RVLLVTTHLPIEAVARAITMEKIYDTIMMGHASIAALDGKTGAIAVTGLDPHCGDAGA
                     IGAFDERVTRPAIERARREGVAVEGPFPADTIFMPGKRDRYALIVA"
BASE COUNT          541 a          692 c          759 g          435 t
ORIGIN      
        1 gttcgaatat cgttccggat tgtttgtatc gcggggttcg acggatcaag cgccgagagc
       61 gttcgtatgc cggagcgggg gccgtcggca aatgccgcgg accaggcaag gaggaccgcc
      121 gcaagggtgg cggccatatg tgctgtcatg gatcgcatga ttggctgatg tatcatctat
      181 cggcagatgt gacataatat attaggcgaa aaacccgacg acggaaaaaa ggtcccggtg
      241 aaaaatatgg ttgaattata tgaaacgcgg tcgctatagt agcccatctg atggcgcgat
      301 tattcgcggg cgcccgtgcg gaaggggcta tggataaaga agaaaaatcc aggcagatgg
      361 tgaaatcgat caagatgctc gagagcgttt ataagacgac cacgagcgcc gcccagcgat
      421 cgcgcgtaaa aaaggacgtc gaaaccctcc gcgctcgttt gaaggaactc taccccgagg
      481 ccaacatcaa ggagctggag gaggcgattt actccgacat gctgacgctc aagtcgaacg
      541 ggacggggat cgacgaggac ctggaattcc ttcgcggcat cgaggtggtc gagtattccc
      601 cgtacaagga cgacgcggag atcaacaccg cagccagcat actcaagcac ttcgaggagc
      661 ggatctgggg cgtgatcacc gaccagcaca ccaagctgga tttctcgaac tcgggagagc
      721 gcgatacgct gtaccgaaag ctcgaccagt gcgaccgcgc cttgaagatg tttctccaaa
      781 ccgtggagga catcgacaag gcgaaatcga acgattacgc gtcgcagctc cagatgatgc
      841 gggtgaagca gggccgggtc ttccagatgg agctcaacga attcctcaag aaggcgcgcg
      901 attttgtggt aaacctggtc tccgacgcgg agttcggggg taccatgatc ctgaaccccg
      961 acgatccgat cgcctatgcc gagtacgaaa cctaccggat gttcgagaac gtcaaggtga
     1021 tcgacgcgct cagattcatg aaaaatttcc tcgaagagac acaaaaggtg atccgcatgc
     1081 ccgatcttgg caaaatgaat aaatgacccc cgccgcggcg cgcgcccgtc tttatgtatt
     1141 gacatgcgcg ctggatgcgg ggcgtagtgg ttaccggcgc gttgggtgcg ccgcgcacaa
     1201 tgaaattcgg tcgaggtatt tatggctatt acgaaaagcg gcggcgatat tgccgataac
     1261 gtgatcggtg agaattcgta tttcaccgga aagttcatca tcaacggctc gctgcgcatc
     1321 gacggccgct tcgaggggaa gtatcttcag gccgagcagc tctatgtcgg cccgaacggg
     1381 aaggtgcgga cgaacatcaa cgccgtttcg ctcatcatcg agggcctcat catcggcaac
     1441 atcaacgcct ccaatcgcgt gctgctcatg cccaccgcga aaatactggg cgacatcaaa
     1501 acgcccgagc tgatcataca aaacggcgtg atcctcgagg gccgctgcac catcgccaac
     1561 gacctccgct cctccgccaa ggacctcatc gaggtcgaat acgacaagaa caagatcatc
     1621 cctgaggagt tcattgcccg cgcgtgacat cgtcgtaggc gtgacggcgg gcgatcccgc
     1681 gggcatcggc cccgaggtcg cctgcagggc gctcatggag cgctacgatg ggatagttcc
     1741 gatcctcatt tgccgcgagg aggtcgtcgc ctccctcggc atcctcgggc gtgatcgtta
     1801 ccgggttgcg cgatcggtcg aagagatcgc ggacccgtcc gatcgcgacc gcattgcgct
     1861 cgtatcgccg gaaagcgacg ctccggtgcc gcgggcgggc gcgggaacgg cggcgaccgg
     1921 cgcggagtcg ctgggctatg tcgataccgc catcgagctt tggcgcgcgg gcgcgatcga
     1981 cgcgatcgtg accggtccgg tgagcaaggc gcttatcgag caatcgggcc atcccttcac
     2041 cgggcatacg gaatatatcg ccgcggccat caacgaaacg acgccctaca tgctgatgta
     2101 ttcggacgaa ctgcgcgtcc tgctagtgac cacgcacctg ccgatcgagg cggtcgcgcg
     2161 cgccatcacc atggaaaaaa tttacgatac catcatgatg gggcatgcgt cgatcgcggc
     2221 gctcgacggc aagacggggg cgatcgcggt gaccgggctt gatccgcact gcggcgatgc
     2281 cggggcgatc ggcgccttcg acgagcgcgt tacccggccg gcaatcgagc gcgcgcgccg
     2341 tgagggggtc gccgtcgagg gcccgttccc ggccgacacg attttcatgc cggggaagcg
     2401 ggaccggtac gcgctcatcg tcgcgca
//
LOCUS       DAORHF010000405         2392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_54104_length_2392_cov_3.553701, whole genome shotgun sequence.
ACCESSION   DAORHF010000405 DAORHF010000000
VERSION     DAORHF010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54104_length_2392_cov_3.553701"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PKJ16_19110"
     CDS             <1..466
                     /locus_tag="PKJ16_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HNV49155.1"
                     /translation="TLNNTQRKSIVITAKKYMGVKYKNGGTTPSGFDCSGYVMYVYNK
                     NGIRVPRAVQEQYKAGKTVPIRHARPGDLVFFTTGRNRYSHVGIYLGNLRFIHAPSTG
                     KTVSVASMKNSYWKSRFIGSVAIVNTKSRPLALSSQQSPSGKHDKNDLEVYN"
     gene            541..1026
                     /gene="coaD"
                     /locus_tag="PKJ16_19115"
     CDS             541..1026
                     /gene="coaD"
                     /locus_tag="PKJ16_19115"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HNV49156.1"
                     /translation="MMRIGIYPGSFDPLTNGHLDIIERAKRLCDRLIIAVARNSSKKP
                     LFSIEERMDMLRACCDTGDNTIEVATFDGLLVDFCKNSDVSFIVRGLRAIVDFDYEYA
                     IALLNRQLAPDIDTIFLMTKGEYSFISSNIVREVASYGGDVSSFVPQFVQQRLQEKLS
                     S"
     gene            1129..2127
                     /locus_tag="PKJ16_19120"
     CDS             1129..2127
                     /locus_tag="PKJ16_19120"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HNV49157.1"
                     /translation="MFKQKVRARPRRIVFPEGDDIRVMKGLAMLSGDGLLGEAVLIGN
                     ADIINENARTVELNKKVFSVIDAGKTAKNPRYADEYRRARNLSVLEQERIMNDVNDPI
                     VLGALMLRLGEVDAYIAGLRTSTAQILRTGLNVIKADRMIGVITAFSMVYADSRERRF
                     KSVIGDEGVILMADPVVNSDPSVGVLCKIAEAAARYLRLYLNLEPRIAFLSYSTKGSS
                     EGRSVDKMRIATERAREKMPEYLIDGELQLDAAISPIASELKAPENALNGNANILIFP
                     NLDAANIGSKLVKHFGNAKLLGPIIYGLQKPFNDISRGANEFDVYDLAIVTQLQVK"
     gene            2212..>2392
                     /locus_tag="PKJ16_19125"
     CDS             2212..>2392
                     /locus_tag="PKJ16_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HNV49158.1"
                     /translation="MELTTLLLIIVLSIMSGIAGFALRAYLGKIKLSSAESRSIRIVQ
                     DAVKEADAKRKELLIE"
BASE COUNT          567 a          657 c          656 g          512 t
ORIGIN      
        1 tactttaaat aatactcagc ggaaatcaat cgtcataacc gccaaaaagt atatgggcgt
       61 aaaatataaa aacggcggca cgacgccgag tggattcgac tgttccggat acgtcatgta
      121 cgtgtacaat aagaacggca tacgagttcc aagggcggta caggaacagt ataaagcggg
      181 aaagacggta ccgattcgac atgcccgccc cggcgatctg gtattcttca caaccggccg
      241 aaatcgatac tcgcatgtgg gcatttatct cgggaatctg cgctttattc acgcaccgag
      301 cacgggaaag accgtctcag tcgccagcat gaagaattcg tattggaaaa gtcgattcat
      361 cggttcggtg gcaatagtaa ataccaaaag caggccgctg gccctttcct ctcaacagag
      421 tccctccggc aaacacgaca aaaatgatct cgaagtctac aattaacttc gcgatttgtt
      481 gttgacaaat atatgtactt aaatagcgtt attttcgtga gaaaagcact ggtgcaattt
      541 ctgatgcgaa ttggcattta ccctggatca tttgatccgt taacaaatgg tcacttagat
      601 atcattgaac gggcgaagcg gttgtgcgat cgtctcatca tcgcggttgc ccggaacagc
      661 tcgaagaagc ccctcttctc gattgaagaa cggatggata tgcttcgcgc atgttgcgat
      721 accggcgata ataccatcga ggtagccacc ttcgacggtt tgctcgtcga tttctgcaag
      781 aatagcgacg tttccttcat cgtccgtggg ttgcgggcga tcgtggattt cgactacgaa
      841 tacgcgatcg cgttgctcaa cagacagctg gcgcccgata tcgataccat tttcctcatg
      901 acaaaaggcg aatattcgtt catctcgtcg aacattgttc gcgaggttgc cagctatggc
      961 ggcgatgtct cgtcttttgt cccccaattc gtccagcaac gcctccagga aaagctttcc
     1021 tcctaacacc gccggcggca tcgttgtgcg ccggatgacg cgttgtcggc tgtattgtcg
     1081 ccgaaacgca cgtacgtgtg agtacgatgg agggacgtca ttctcgaaat tttcaaacag
     1141 aaggttcgcg cgcgcccgcg aaggatcgtt ttccccgagg gtgacgacat tcgcgtgatg
     1201 aagggccttg caatgctgag cggtgacggc ctgctcggag aggccgtgct catcggcaat
     1261 gccgacatca tcaacgaaaa cgcccgcacg gtcgagctga acaaaaaggt cttctcggtc
     1321 atcgacgccg gtaaaacggc gaaaaatccc cgatatgccg acgaatatcg ccgcgccagg
     1381 aacctgagcg tcctcgagca agaacgaatc atgaacgacg tgaacgatcc gatcgttctg
     1441 ggtgcgctca tgctgcgcct gggggaggtg gacgcgtaca tcgccggcct tcgcaccagc
     1501 accgcgcaaa tcctgcggac gggattgaac gtcatcaagg ccgaccggat gatcggcgtg
     1561 attaccgcat tcagcatggt gtacgcggat tcgcgcgaac gccgattcaa gtcggtcatc
     1621 ggcgacgagg gcgtgatcct catggcggac cccgtggtca attccgaccc atccgtgggc
     1681 gtgctctgca aaatcgccga ggccgccgca cgctacctgc ggctgtatct caatctcgaa
     1741 ccgcggatcg cgttcctttc ctactccacg aaggggagct cggaagggcg gtcggtggac
     1801 aagatgcgca tcgccaccga gcgcgcccgc gagaagatgc ccgagtacct catcgacggc
     1861 gaactgcagc tcgacgcggc catctcgccg atcgccagcg aactgaaggc cccggagaac
     1921 gcgctgaacg gaaacgcgaa catactcatc tttccgaacc tcgacgccgc gaatatcgga
     1981 tcaaaactgg taaagcactt cgggaacgcg aagctcctgg ggccgatcat ttacggactg
     2041 caaaagccgt tcaatgacat ctcccggggg gcgaacgaat ttgacgtgta cgatttggct
     2101 attgttaccc agctgcaggt caagtaggcc tgccgccggg cgtgtttgtt gtttggtcgt
     2161 atcgcgcgca cgcatttcgc gcgacactca cgtaaccgtg gaggaaatca catggaactt
     2221 acaacgctcc tcctgatcat cgttctctcg atcatgtctg gaattgccgg gtttgccctg
     2281 cgcgcgtacc tgggtaaaat aaagctgtcg tcggcggagt cgcgctccat ccgcatcgta
     2341 caggacgcgg tcaaggaggc ggacgcaaag cgcaaggagc ttctcatcga gg
//
LOCUS       DAORHF010000406         2383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_54415_length_2383_cov_3.528351, whole genome shotgun sequence.
ACCESSION   DAORHF010000406 DAORHF010000000
VERSION     DAORHF010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54415_length_2383_cov_3.528351"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="PKJ16_19130"
     CDS             complement(<1..692)
                     /locus_tag="PKJ16_19130"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HNV49159.1"
                     /translation="MKTEHPGDSRGGVQFRRELRLLDATLLVMGSMIGSGIFIVSADI
                     ARNIGAPAYLLLVWIIAGLITVVGALSFGELVGLMPWAGGQYVFIREAYNPLTAFLYG
                     WSLFMVIQAGTIAAVGMAFAKFTAVVYPALGEGVKIFVLGRFSVSAAQCVAIASIALL
                     TYMNTRGIRGGKIVQDIFTLAKVAALVGLIVLGILIGRDHDVIARNLAGFWDAHMVRA
                     VDGTIVSMEALSG"
     gene            complement(763..1569)
                     /locus_tag="PKJ16_19135"
     CDS             complement(763..1569)
                     /locus_tag="PKJ16_19135"
                     /EC_number="1.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245230.1"
                     /note="This oxidoreductase belongs to a branch of the SDR
                     family in which the NAD cofactor is especially deeply
                     buried and is non-exchangeable. Members of this branch
                     occur only in species that product mycofactocin, a small
                     molecule electron carrier derived from the final two
                     residues of the mycofactocin precursor protein, MftA.
                     Mycofactocin is thought to mediate transfers of electrons
                     between such non-exchangeable NAD cofactors from different
                     enzymes acting on different substates, and has been shown
                     to play a role in the metabolism of alcohols and aldehydes
                     in Mycolicibacterium smegmatis and in Mycobacterium
                     tuberculosis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HNV49160.1"
                     /translation="MAGECEGRVAFITGAAHGQGRAAALALAREGADIVGFDVAKRIE
                     YPVYEFGTGEELESLKNEVEALGRRALVFAGDVRDDAAIVRAVDATVAEFGRIDILFN
                     NAGICAYAMAHEMTELEWDAMIDINLKGAWLVARRIIPHMIRERSGVIINNSSVMGLR
                     GGNRLSHYAASKWGMIGLTKSWAIELAPHNVRVVSIHPTGVNTPMNDGLAFMEGATPI
                     EIAERSAGNLLPVPWVEPEDVAHMVVYLSSDRARYVTGAQFVLDAGLLSR"
     gene            complement(1571..2110)
                     /locus_tag="PKJ16_19140"
     CDS             complement(1571..2110)
                     /locus_tag="PKJ16_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49161.1"
                     /translation="MSYISMTEYETAGEEVRREYDDQIARHGRITNMKRTLLHSVPAF
                     RAYMEWYTLRDLIVPFIGERALSLFSYAISNGNNCLICSLFFRKILVDSGEDPDNPRL
                     NDVERLLMELGRRIAVDPHAITDDVYDGLKLKFTEEQIVLLVAFAGIMYATNLFNTIA
                     KVPLDEVLYPYRGKQTGEE"
BASE COUNT          433 a          807 c          703 g          440 t
ORIGIN      
        1 cccgaaaggg cttccatcga tacgattgta ccgtccacgg cgcgcaccat gtgcgcgtcc
       61 cagaagcccg cgaggttccg ggcgataaca tcatggtcgc gaccgatgag gatcccgaga
      121 acgatcagcc cgacgagcgc ggcgaccttg gccagtgtga agatgtcctg cacgatcttc
      181 ccgccgcgga tcccccgcgt gttcatgtat gtcagcagcg cgatgctcgc gatagccacg
      241 cactgcgcgg ccgacacgct gaagcggccg aggacgaaaa tcttcactcc ctcgcccagc
      301 gcggggtaga cgaccgccgt gaacttggcg aaggccattc ccaccgccgc gatggttccc
      361 gcctggatga ccatgaagag cgaccagcca tagagaaagg ccgtaagcgg attgtatgcc
      421 tcgcggatga agacgtactg cccccccgcc catggcatga ggcccacaag ctcgccgaag
      481 ctgagcgctc cgaccaccgt gatgaggccg gcgataatcc atacgagcag caggtaggcc
      541 ggcgccccga tgttgcgcgc gatgtcggcg ctcactatga agatgcccga gccgatcatg
      601 gagcccataa cgagcagcgt cgcgtccaga agcctcagct cgcgcctgaa ttgtacgcct
      661 ccacgcgaat cgccgggatg ttccgtcttc atggctcctc cttgcaggta tgggaagacc
      721 gtaaccactg gttccgtctt catccgccgg agcgggtcgg ccctatctcg acagaaggcc
      781 cgcgtcgagg acgaactggg cgccggtaac gtagcgcgcc ctgtcgctcg aaagatagac
      841 caccatgtgc gcaacgtcct ccggttcgac ccatggaacg ggcagaaggt tccccgccga
      901 gcgctcggcg atctcgatcg gagtggcgcc ctccatgaac gccaggccgt cgttcatcgg
      961 agtgttgacc cccgtgggat ggatgctcac cacgcgcacg ttgtgcggcg cgagctcgat
     1021 cgcccacgac tttgtcaggc cgatcatgcc ccacttggac gcggcgtaat gggagagcct
     1081 gttcccgccg cgcagcccca tcaccgacga attgttgatg atgacccccg agcgctcccg
     1141 gatcatgtgc gggatgatcc gccgtgccac gagccacgcg cccttcaggt tgatgtcgat
     1201 catggcgtcc cattcgagct cggtcatctc gtgcgccatg gcgtaggcgc agatgcccgc
     1261 gttgttgaag aggatatcga tcctcccgaa ttccgcgacc gtcgcgtcga cggcccgcac
     1321 tatcgccgcg tcgtcacgca catcgccggc gaacaccagc gcccttcggc ccagcgcctc
     1381 gacctcattt ttcagcgact cgagctcctc gccggtgccg aactcgtaga ccggatactc
     1441 gattcttttc gccacgtcga agccgacgat gtcggccccc tcccgcgcga gcgccagcgc
     1501 cgccgcgcgt ccctgaccgt gcgcggcccc ggttatgaac gccaccctgc cttcacattc
     1561 ccccgccatc tcactcctcc cccgtttgtt tgccccggta ggggtacagc acctcgtcga
     1621 gcggcacctt cgcgatcgta ttgaaaagat tcgtcgcgta cattatcccg gcgaaggcga
     1681 caagcaggac gatctgttcc tcggtgaatt tcaatttgag gccgtcgtag acatcgtcgg
     1741 ttatcgcgtg cggatccacc gcgatccggc ggcccagctc cataagcagg cgctcgacgt
     1801 cgttgagcct cgggttgtcc gggtcctccc cgctgtccac cagtatcttg cggaaaaaga
     1861 gcgagcagat gaggcagttg ttgccgttgg agatcgcgta ggaaaagagc gagagggccc
     1921 gctccccgat gaagggcacg atgaggtccc ggagcgtata ccattccatg taggccctga
     1981 aggccggcac ggaatgcagc agggtgcgct tcatgttggt gatacggccg tgccgggcga
     2041 tctggtcgtc gtactcgcgg cggacctcct cgcccgcggt ctcgtactcg gtcatcgaaa
     2101 tatagctcac aacgccctcc cggatgctcg tgatttcaat gcctgccggg catccaatta
     2161 ccaccccgcg gacgggatgt ccagccaatt aatggaacat tgcgcgcccg tcccccggaa
     2221 gatgtttgac ggcctcgcgc cgcgccggta ccatgtgtcg ccatgaaagc attcgccggt
     2281 acgatctgtg cgcttaccat ccttttcgcc tccgccggcg gtcgcggggc ggtgatgttc
     2341 gatcccgccc tggactggcg ctccattaag accgaacact tcc
//
LOCUS       DAORHF010000407         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_55064_length_2364_cov_4.895626, whole genome shotgun sequence.
ACCESSION   DAORHF010000407 DAORHF010000000
VERSION     DAORHF010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55064_length_2364_cov_4.895626"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2364)
                     /locus_tag="PKJ16_19145"
     CDS             complement(<1..>2364)
                     /locus_tag="PKJ16_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49162.1"
                     /translation="DGLDAIIVELNGYKDIIAQRVTALRAKEDDLNAKKQAYEDMRLM
                     YVLEANEDFSAEATVSKETLDALQTASAAYYAVQQEYNALRAELEQARTDYNTRSGDY
                     TAQMNTIADLYKTYQEREFEYEKAYAVWEYANTPYLKGSQNYDAGLTTGSVPGSGTGD
                     FAGMTSPDARENYEHHLTQYNTAHDVWAQAKDRMDNQETVEMLYAMDGTYAGLRDAAA
                     TAVRGYADGGSDPTAAVNEYLNYCAGAAMLGLHDQTVLLRDQEGLFYELLQYSKDRDS
                     DAAYNYTINALVSNGSGTSVGDRIVSGMLEQNRLFQEQNWDQRQATMDERERRWQEVV
                     GFIKNRGERDWTNNLLSFENAWAKWREEMRAEITKGEEKWTGVFNEFNGRMANWQVKM
                     AEAASDAGSKRMVEDLMADMQRYIDGLNRNLPGSARLDFDAQGLVTRLTQKIPEGIGV
                     LADSMRFVDTTAGFTNIFSLGLNYTDAFSSFEKKMKSFQEGMEKMRAVRQGEAAYQVF
                     LAMIDEFNRQLAEMNTNNYGRVESEIRSQYKAPFRRGWKLSVTTGKTMMGGREKDEIS
                     FSDYRSYVNTTVFLKPLKGIEGNVDFRNPYTYQNLNERELGLFVKLETDFLNREIDRV
                     FRPGGYFESHTTREFGRLHRDFADAYSKYQGGKSFEKMNGFDAPIVAGAPSMRMVTQI
                     SASIAANILLPGSGAAVTAAFTAYDVSQGKLSATQAITETGIGFVAGAAGPMGGMFVN
                     TLAQGFKYGKSGGLSFDLNNISQDAWKTAVANAAVSGAIGNGIASKF"
BASE COUNT          406 a          802 c          610 g          546 t
ORIGIN      
        1 cgaactttga agcgatcccg ttgccaatgg cgcccgagac tgccgcgttg gccaccgccg
       61 tcttccaggc gtcctgagag atgttgttta ggtcgaaact taatccgccg gattttccgt
      121 acttaaagcc ctgtgcgagt gtattcacga acatgccgcc catggggccg gccgcaccgg
      181 caacaaatcc aatacccgtc tcggtaatcg cctgcgtcgc gcttaactta ccctgagaca
      241 catcataagc agtaaatgct gctgttactg cggcccccga tccgggcaag agtatatttg
      301 ccgcgatgct cgcgctaatc tgcgttacca tccgcatgct cggcgccccc gccacgatgg
      361 gcgcatcaaa gccgttcatt ttttcaaaag acttgccgcc ctgatacttc gaataggcgt
      421 cggcgaaatc gcggtgcagt cgtccaaact cccgcgtcgt gtggctctcg aaatacccgc
      481 ccgggcggaa cacgcggtct atctcgcggt tcaggaagtc cgtctccaac ttcacgaaca
      541 ggcccagctc gcgctcattg aggttctgat aggtgtaggg attgcggaaa tcgacgttgc
      601 cctcaatccc cttgaggggt ttcaggaaca ccgtggtgtt cacataactc cggtaatcgg
      661 aaaagcttat ctcgtccttc tcccggccgc ccatcatggt ctttcccgtg gtcaccgaaa
      721 gtttccagcc gcgcctgaac ggcgctttgt actggctccg gatttccgac tcaacgcgcc
      781 cgtagttgtt cgtgttcatc tcggccagct gccggttaaa ctcgtctatc atcgccaaaa
      841 acacctggta ggccgcctcg ccctggcgca ccgcccgcat cttctccatc ccctcctgga
      901 aggacttcat cttcttctcg aaggagctga aggcgtcggt gtaattgagc cccaggctga
      961 atatgttcgt aaatcccgcc gtcgtgtcca caaaccgcat cgaatcggcc agcacgccaa
     1021 tcccctcggg tatcttctgc gtaagccgcg tgaccaatcc ctgcgcgtca aaatcgagac
     1081 gcgcgcttcc cggcaggttc cggttgagac cgtcgatata gcgctgcatg tccgccatca
     1141 ggtcttccac catgcgcttc gatcccgcat ccgacgcggc ctcggccatt ttcacctgcc
     1201 agttcgccat ccggccgtta aattcattga aaacgcccgt ccacttctcc tcgcccttcg
     1261 tgatctcggc gcgcatctcc tcgcgccatt tcgcccaggc gttctcgaag gacaggagat
     1321 tgttcgtcca atcgcgctcc ccacggtttt taataaaacc aaccacttcc tgccagcgcc
     1381 gctcgcgctc gtccatcgtg gcctgccgct gatcccagtt ctgttcctga aacagccggt
     1441 tctgctcaag catgccggac acgatccggt cgccaacgct cgtcccgctg ccgttggaca
     1501 ccagcgcgtt tatcgtataa ttgtacgcag cgtccgaatc gcggtccttc gagtactgca
     1561 acagctcgta aaacagcccc tcctggtcgc gcaacagaac cgtctggtcg tgcaggccca
     1621 gcatcgccgc gccggcgcaa taatttaaat attcgttcac cgccgcggtc ggatcggatc
     1681 cgccgtcggc gtagccgcga accgcggtcg cagccgcgtc gcgaagtccg gcgtatgttc
     1741 cgtccatcgc gtagagcatc tcaaccgttt cctggttgtc catccggtcc ttcgcctgcg
     1801 cccatacgtc gtgcgccgtg ttgtactgcg tcaggtgatg ctcgtagttc tcgcgcgcgt
     1861 cggggctggt catccccgcg aagtcgcccg tgccggaccc gggaacgctc cccgtggtga
     1921 gtcccgcatc gtagttctgg cttcccttca gatacggcgt attcgcgtac tcccataccg
     1981 cgtaggcctt ctcgtactcg aactcgcgct cctgataggt cttgtagaga tcggcgatgg
     2041 tgttcatctg cgccgtgtag tcgccgctgc gggtgttata gtcggtccgc gcctgctcca
     2101 gctcggcccg cagcgcattg tattcctgct gcaccgcata gtaggccgcg ctcgccgtct
     2161 gcagcgcgtc cagcgtttcc ttgctcaccg tggcctcggc cgaaaaatcc tcgttcgcct
     2221 ccagcacata catcaggcgc atgtcctcgt acgcctgctt cttcgcgttc agatcgtcct
     2281 ctttggcccg cagcgccgtc acccgctggg cgatgatgtc cttgtagccg ttcagttcca
     2341 cgatgatcgc gtcaagtccg tcgt
//
LOCUS       DAORHF010000408         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_55629_length_2349_cov_4.252398, whole genome shotgun sequence.
ACCESSION   DAORHF010000408 DAORHF010000000
VERSION     DAORHF010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55629_length_2349_cov_4.252398"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..803)
                     /locus_tag="PKJ16_19150"
     CDS             complement(18..803)
                     /locus_tag="PKJ16_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV49163.1"
                     /translation="MDDLLQVDGLSLSFGGLRAVHGIRFSVKPGMIFSIIGPNGAGKT
                     TIFNCISGIYRPNEGRISFAGRDITRLAPHAIARLGIARTFQNIELFSRMTAMENIML
                     GRHVHMKRGLLRGAFMWGRRSAAAREECENRLVAERIIDLLDLQSARDHFVGELPYGR
                     QKLVELARALALEPTLLLLDEPSAGMNSEEKQDLIFWIKDIQEEMGITILLIEHDMKM
                     IMDISDRVLAINFGEPIIEGAPAEVANHPDVLRAYLGEDQASA"
     gene            1033..1956
                     /locus_tag="PKJ16_19155"
     CDS             1033..1956
                     /locus_tag="PKJ16_19155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HNV49164.1"
                     /translation="MNKKKTILIIVAVILLIPAIVFFGFPQVILNMSVNKARKAAGLT
                     AKTVVVDEFTVAYLEGGSGEPVIMLHGLGGDKDNWAAFAKTITPSYRVIALDLPGSGE
                     STKNLAALYGIALQSERLGKIASALGAAKFHLVGNGMGSVIASHFAISKPDKVLSLTL
                     INTEAVPCPERSEFEKQVAVGKNPLSVTNVADFDNLLKFMFVTPPSIPGPIKRYLTKK
                     SIANSDFNQKIFWDVFGEDYKLAGELGKIKSRTLVMWGEKDNRVHVSCTKLLEKGISK
                     SAVVIIKDCGHFPMREKPDDAAKEFLKFVKG"
     gene            complement(2022..>2349)
                     /locus_tag="PKJ16_19160"
     CDS             complement(2022..>2349)
                     /locus_tag="PKJ16_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49165.1"
                     /translation="FDDAETKYVPQGESGGAQNVIRTRTNNSAKIAYGTYFRRQGLQL
                     NDVFVGINLSLTPDVLFSFQFIHEEEYNQNYDPKSELASERVRLLQINRYLSMGVTYT
                     LDFSGK"
BASE COUNT          519 a          680 c          630 g          520 t
ORIGIN      
        1 tctcgacatt gttgatctta agcactggcc tggtcctccc cgaggtaggc ccgcaggaca
       61 tcgggatgat tcgccacctc cgccggcgcg ccctcgataa tgggctctcc gaaattgatg
      121 gccagcacgc gatcggatat gtccatgatc atcttcatgt cgtgctcgat aaggaggatg
      181 gtgatgccca tctcctcctg tatgtccttg atccagaaga tgaggtcctg cttctcttcg
      241 ctgttcatgc cggccgacgg ctcgtccagg agcagcagcg tcggctcgag cgcaagcgcg
      301 cgcgcaagct ccaccagttt ctgccgtccg tacggtagct cgcccacgaa gtggtcgcgc
      361 gccgactgca ggtccagcag atcgatgatc cgctctgcga ccaggcggtt ttcgcactcc
      421 tcgcgcgcgg cggcagaccg tcgcccccac atgaaagcgc cccgcaggag gccgcgcttc
      481 atgtgcacat gccgccccag catgatgttt tccatcgcgg tcatgcgcga aaagagttcg
      541 atattctgga aggtgcgcgc aatgccgagg cgcgcgatgg catggggcgc gagacgggtg
      601 atgtcgcgtc ccgcgaatga tatgcgccct tcattgggtc gatagatacc gctgatgcag
      661 ttgaatatcg tggttttccc cgcaccgttg ggaccgatga tggagaaaat catgccgggc
      721 ttcacggaaa agcgtatccc atgcacggca cgcaaaccgc cgaagctcag cgacaggcca
      781 tcgacctgga gtaaatcatc catgagggag taacggtatg tccttccaat tatttatcgc
      841 tatgctaagt taatctcgct gtatcgttat caccctgtca aatcaatttt cattttttcg
      901 tatgattcat agtacgatcg ttgatttctt cttgactgat ataaaatcac actgtagata
      961 tgtgtaaaaa gacaccacag atatacacac agccaaggcc atatcctata acatttcgag
     1021 cgaggtaaac ctatgaataa aaagaaaacc attctcatta tcgtagcggt cattctcctc
     1081 atcccggcca ttgtcttttt cggattcccc caggtcattc tcaacatgtc ggtgaacaag
     1141 gcgcgtaagg cggccggtct taccgcgaaa accgtcgtcg ttgatgaatt taccgtggca
     1201 tatctcgagg ggggcagcgg cgaacccgtc atcatgctcc atgggctcgg cggcgacaag
     1261 gacaactggg cggccttcgc caagaccatc acgccgtcgt atcgcgtcat tgcgctcgac
     1321 ctgcccggat ccggcgaaag cacgaagaac cttgccgccc tgtacgggat cgcgcttcaa
     1381 tcagagcggc tcggaaagat cgcctccgca ctgggggccg cgaagttcca tctcgtgggc
     1441 aatgggatgg ggagcgttat cgccagccat ttcgccatct ccaaacccga caaggtgctg
     1501 tcgctcactc ttatcaacac cgaggccgtg ccgtgccctg agcgaagcga gttcgaaaag
     1561 caggtcgccg tggggaaaaa cccgctttcg gtcacgaacg tcgccgattt cgacaacctg
     1621 ctcaaattca tgttcgtaac gccgccgtcg atccccggcc ccatcaagcg ctatctgacg
     1681 aaaaaatcca tcgcgaactc ggacttcaat caaaagatct tctgggacgt gttcggcgag
     1741 gactataagc ttgccggaga gcttggcaag atcaagtccc gaacgctggt catgtgggga
     1801 gaaaaggaca atcgggtcca tgtttcctgc acgaagctgc tcgagaaggg gatttcgaaa
     1861 agcgcggtgg tcatcatcaa ggactgcggc catttcccga tgcgcgagaa accagacgac
     1921 gcggcgaaag agttcctgaa gttcgtcaaa ggctgattcc cctgcggggt atttgccgcg
     1981 cccgggcagc atgcgtttta tgcgatctcg ggtgcggccc cctacttccc gctaaaatcc
     2041 agcgtatagg tcacacccat cgagagatac ctgttgattt gcagcaaccg aacccgctcc
     2101 gacgccagct cgcttttcgg atcgtaattc tgattgtatt cctcttcgtg gatgaactgg
     2161 aacgaaaaaa gaacatccgg cgtgagcgat agattgatcc cgacgaatac gtcgttcaac
     2221 tggagtccct ggcggcggaa ataggtccca taggcgatct tcgcgctgtt attggtccgc
     2281 gtacggatca cgttttgcgc gcctcccgat tcgccttgcg gcacatactt tgtctccgca
     2341 tcatcgaac
//
LOCUS       DAORHF010000409         2317 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_56848_length_2317_cov_4.671972, whole genome shotgun sequence.
ACCESSION   DAORHF010000409 DAORHF010000000
VERSION     DAORHF010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56848_length_2317_cov_4.671972"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="PKJ16_19165"
     CDS             <1..306
                     /locus_tag="PKJ16_19165"
                     /inference="COORDINATES: protein motif:HMM:NF012662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49166.1"
                     /translation="EDLFKSFADVVEYDFEKVLCVSANTKKHDIAEVFKAFNSINFDS
                     VIITKVDETGYIGSVVDVADRFKKPIAYYTFGQEVPNDIAIAESERMVDLMFGNAHN"
     gene            322..1221
                     /locus_tag="PKJ16_19170"
     CDS             322..1221
                     /locus_tag="PKJ16_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HNV49167.1"
                     /translation="MDQAANLRRLVLENNAVRKCKTIAITSGKGGVGKSTFSVSLAIA
                     LAQNGASVTLLDADLGLANVNVILGIIPKFNLYHVIKGKKSLKEIIIDVPEGIKIIAG
                     ASGFHQLANLEDKQLAKFIETIGEIDTDDYMIIDTGAGVSQNVLSFVIAADEVLVVTT
                     PEPTAITDAYGIIKSIASHTSDKNIKLVVNRVQSVSEGQRVAQRVINIAGQFLNIKVE
                     NLGFIFDDISVQKSIRNQKPFMVSYPKTKASVCVKLIADRICNRDVEAERGTGLVSFF
                     RNMLRSSKDAQSVAEAVAEAPSA"
     gene            1284..1778
                     /locus_tag="PKJ16_19175"
     CDS             1284..1778
                     /locus_tag="PKJ16_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49168.1"
                     /translation="MDIKELLPSFGGIDAKIRTALAFGATAFIISLFVGLVSGNFITG
                     ALATSLVMAAVFMVLGYAAVFVITRYVPEVLALFTRTGSGTGGESESAAPAGGETATN
                     EPEPTASAEFKPMTDDSFTRMESVPASGDGQLGKHIVKKERKIKYEPKLMAEAIRTMI
                     KKDE"
     gene            1814..>2317
                     /locus_tag="PKJ16_19180"
     CDS             1814..>2317
                     /locus_tag="PKJ16_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HNV49169.1"
                     /translation="MRNKKYEEFDKVDEDKLWKQFAKSKDNDLRNYFVEKYAPLVKYV
                     AGKISIGMPHNIEFDDLVSYGVFGLIDAINKFDFNRNIKFKTYAMTRIRGAIFDELRS
                     IDWIPRSIRQKAKQLEQVIAELENKLGRTVEDEEIARELGVSSEEFQTLLNKLSGTAL
                     MSLNDIWY"
BASE COUNT          553 a          639 c          699 g          426 t
ORIGIN      
        1 gaggaccttt tcaagagctt cgcggacgtg gtggagtacg atttcgaaaa ggtcctctgc
       61 gtgagcgcga acaccaagaa gcacgatatc gccgaggtgt tcaaggcgtt caacagcatc
      121 aatttcgaca gcgtcatcat caccaaggtg gacgagaccg ggtacatcgg cagcgtggtc
      181 gatgtggccg atcgcttcaa gaagccgatc gcctattaca ccttcggcca ggaggtcccc
      241 aacgacatcg cgatcgcgga atcggaaaga atggttgact tgatgttcgg caacgcgcat
      301 aattagatcg ggaggcgcaa cgtggatcag gcagcgaact tgagaagact ggtactggag
      361 aacaacgccg tcaggaagtg caagaccatc gccatcacca gcgggaaggg gggcgtgggg
      421 aagtccacgt tttcggtgag cctggcgatc gcgctggcgc aaaacggtgc gagcgtcacc
      481 ctgctcgacg ccgacctggg ccttgcgaac gtgaacgtga tcctggggat catccccaag
      541 tttaacctgt atcacgtcat caagggcaag aagtccctca aggagatcat catcgacgtc
      601 cccgagggga tcaagatcat cgcgggggcg tccggattcc accagctggc gaacctcgag
      661 gacaagcagc tggcgaagtt catcgagacc atcggcgaga tcgacaccga cgattacatg
      721 atcatcgaca ccggcgcggg ggtttcgcaa aacgtgctct cgttcgtgat tgcggccgac
      781 gaggtgctcg tcgtgaccac gccggagccc acggcgatca ccgacgcgta cggcatcatc
      841 aagtcgatcg cctcgcacac ctcggacaag aacatcaagc tcgtggtgaa ccgcgtgcag
      901 tccgtttcgg aggggcagcg cgtggcgcaa cgcgtcatca acatcgccgg gcagttcctg
      961 aacatcaagg tcgaaaacct ggggttcatc tttgacgata tttcagtgca gaaatcgatc
     1021 agaaaccaga aacccttcat ggtgtcgtat ccgaaaacga aggcgtcggt atgcgtgaag
     1081 ctcatcgccg atcggatttg caaccgcgat gtggaggcgg agcgcggcac cggcctggtg
     1141 tccttcttcc gcaacatgct gcggtcgtcg aaagacgcgc aatccgtcgc cgaggcggtc
     1201 gcggaagcgc catcggcgta aaaaaagggt tgaagaaaaa cttgtgaata tttaccttat
     1261 atataccggg tagcgggagc gggatggata tcaaggagct cctgccgtcg ttcggtggca
     1321 ttgacgcaaa aattaggacc gcgctggcat ttggcgcaac ggcgttcatc atttcactgt
     1381 ttgtcgggct tgtatcggga aacttcatca ccggcgcgct ggcgacttcc ctggtgatgg
     1441 cggccgtgtt catggttctc ggctatgccg ccgttttcgt catcacccgc tacgtcccgg
     1501 aggttcttgc gctcttcacg cgaacggggt cggggaccgg aggggagagc gaaagcgccg
     1561 cacccgccgg aggcgagacg gcgacgaacg aaccggagcc gacagcctcg gcggaattta
     1621 agccgatgac cgatgatagc ttcacccgca tggagtcggt tcccgcttcg ggcgacgggc
     1681 agctggggaa gcatatcgtg aagaaggagc gtaagatcaa gtacgagccg aaattgatgg
     1741 ccgaggccat caggaccatg atcaagaagg acgagtagtc gcgttcggtt ataccaagca
     1801 ggcaagggac ggtatgcgaa ataagaaata cgaagagttc gacaaggtag acgaggacaa
     1861 gctctggaag cagttcgcca agtccaagga caacgacctg cggaactatt tcgtcgaaaa
     1921 gtacgccccg ctcgtcaagt atgtcgccgg caagatctcc atcggcatgc cgcacaatat
     1981 cgagttcgac gatctggtct cctacggcgt gttcggcctc atcgacgcca tcaacaagtt
     2041 cgattttaac cgcaacatca agttcaagac ctacgcgatg acccgcatcc gcggcgcgat
     2101 cttcgatgag ctgcgctcca tcgactggat cccccgctcg atccgccaga aggcgaaaca
     2161 actcgaacag gtgatcgccg agctggagaa caagctgggg cgcaccgtcg aggacgagga
     2221 aatcgcccgc gagctgggag tgtcgagcga agagttccag accctgctca ataaactctc
     2281 cggaaccgcc ctcatgtcgc tcaacgacat ctggtat
//
LOCUS       DAORHF010000410         2310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_57111_length_2310_cov_4.328160, whole genome shotgun sequence.
ACCESSION   DAORHF010000410 DAORHF010000000
VERSION     DAORHF010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57111_length_2310_cov_4.328160"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="PKJ16_19185"
     CDS             <1..555
                     /locus_tag="PKJ16_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49170.1"
                     /translation="GVSITIAKAAMLIGVSALFLFGGAASSVYYAVRALTAPDSEIRV
                     QSEESGQSVASSQALVSNTTAPTAAADPPELVERRFGVQGFTAANVDPATAARVSGMV
                     ASELAKLKGGERVIRGASKDRKAGMLVLGSVEELGGAYDVTARVVSVKDGRILFYSSE
                     TAGSAAELDGAAVRLSRKIAAGIR"
     gene            565..1605
                     /locus_tag="PKJ16_19190"
     CDS             565..1605
                     /locus_tag="PKJ16_19190"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HNV49171.1"
                     /translation="MSRRADRYSGMGAVLAGVSIILFCVVMMVPARSLAREAAKGKKR
                     IALLEFEANNTTPGVARIVRNTVEMNLFQSGHFDVLERSQMDLILEERKTQMRACRDE
                     SCAGELGEILATDFVIVGSVDVLETTKVSVKVIDVKTKRVVLAETRETDKVTEVRGVL
                     KAVSEKIADRLRRGDLDGAGWLGNAVPAIDVRVLYAQSYGYLKDVLGAGYGLSVTGAL
                     ENIFITDFVLRVSLSYFHFAGLENKAHHARFIPLSIGAGYVLRLGPVEFLPSVGVGLS
                     QNSLYYYTDAAGTAYAEKTRSQPIVIGDARVRYFFKDSFFAQAGFEYANVFEKSGDVT
                     FISIFGGAGMRF"
     gene            1848..>2310
                     /locus_tag="PKJ16_19195"
     CDS             1848..>2310
                     /locus_tag="PKJ16_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49172.1"
                     /translation="MKRFMAVMMAVMMVGGLFACTGKKGSKGIPGGSGSSIDRSGSAF
                     VSAQDGGTVVYNDEVALTVPENSIQQDATITVEKTSEIPAGQSDSLVHAGTAYKFGPE
                     GTSFDPSKPAVLEISYNEQALVAKGLSPQTLQIYYFNTELQQYVAVPSYVDD"
BASE COUNT          424 a          639 c          750 g          497 t
ORIGIN      
        1 ggagtatcga tcaccatcgc gaaggcggcc atgctcatcg gcgtctcggc gctcttcctg
       61 tttggcgggg ccgcgtcgag cgtgtattat gccgtccgcg cgctcacggc gccggattcg
      121 gagatccggg tccaatcgga agaatccggg caatccgtcg cgagcagtca ggcgctcgtc
      181 tcaaacacga ccgctcccac ggctgcggcc gatccgcccg agctcgttga gcgacgcttc
      241 ggcgtgcagg gcttcacggc ggccaatgtc gatccggcca cggccgcgcg cgtgagcggc
      301 atggtcgcct cggagctcgc gaaactcaag ggcggcgagc gcgtcattag gggcgcgtcg
      361 aaggaccgaa aggcgggcat gctggtgctg ggctcggtgg aggagctcgg cggcgcctac
      421 gacgtaaccg cgcgcgtggt gagcgtgaag gacggccgca tcctgttcta ctcgtcggaa
      481 acggccggga gcgcggccga gcttgatggc gcggcggtgc gcctgtcgcg caagatcgcc
      541 gccggtatcc gttaggcgat attcatgtcc cgcagggcgg atcgctactc cggaatgggc
      601 gccgtgctgg cgggagttag tatcatactt ttttgtgtgg tgatgatggt tcccgcccgt
      661 agcctggccc gcgaggccgc gaagggcaag aagcgcatcg ccctgctcga attcgaggcg
      721 aacaacacca cgccgggcgt cgcgcgcatc gtccgcaaca ccgtggagat gaacctcttt
      781 cagtccggcc acttcgacgt ccttgagcgc agtcagatgg acctcatcct cgaggagcgc
      841 aagacgcaga tgcgcgcctg ccgggacgag tcgtgcgccg gggagctggg cgagatcctc
      901 gcgaccgatt tcgtgatcgt ggggagtgtg gacgttctgg agaccaccaa ggtgagcgtg
      961 aaggtgatcg acgtgaagac caagcgcgtg gtgctggccg agacgcgcga gaccgacaag
     1021 gtgacggagg tgcgcggcgt ccttaaagcg gtctcggaaa agatcgccga tcggctccgg
     1081 cgcggcgacc tcgacggcgc ggggtggctg ggtaacgccg ttcccgcgat cgatgtgcgg
     1141 gtcctctacg cgcaatccta tgggtacctc aaggacgtgc ttggggccgg gtacgggctc
     1201 tcggtgacgg gcgcgctcga gaatattttt atcaccgatt tcgtcctccg cgtttcgttg
     1261 tcctactttc acttcgccgg tctggagaac aaggcccatc atgcgcgctt cattccgctt
     1321 tcgattggcg cgggatatgt cctgcgcctg ggccccgttg agtttctgcc gtcggttggc
     1381 gtggggttga gccagaacag cctgtactat tacaccgacg cggccggcac cgcctatgcc
     1441 gaaaagaccc gctcccagcc gatagttatc ggtgacgccc gagtgcgcta cttcttcaag
     1501 gattccttct tcgcgcaggc gggttttgaa tatgccaacg tattcgagaa atccggcgac
     1561 gtgaccttca tttccatttt cggaggggcg gggatgcggt tttagggcga gttgttatcg
     1621 gttttttcgg aaggtttttt tatgaggctc tgaccctttg atgggcattt aaataaacat
     1681 ttttcggaat acgtcttttt tgttccgtga gaattatcac tttccttccc tggcgaacgt
     1741 catttcttcc cccggaaatt ttttgaaaaa ttttgtcact ccactcgtac ttccgacgct
     1801 tctccggcaa agcagcatat ttcatagata cgtcagagga gcaacagatg aagcggttta
     1861 tggcagtgat gatggccgtg atgatggtgg gcggcctgtt tgcgtgtacc ggcaagaagg
     1921 gcagtaaggg tattccgggc ggaagcggta gttcgattga tcgctcgggc agcgcgttcg
     1981 tgtcggcgca ggatggcgga actgtcgtgt ataacgatga agttgcgctc acggtgccgg
     2041 agaactcgat ccaacaggat gccacgatca ccgtggagaa aacatccgag atccccgcgg
     2101 ggcaatcgga cagtctggtt cacgcgggaa ctgcttataa gtttggcccg gaaggaacgt
     2161 cgtttgatcc gagcaagccg gccgttctcg agatcagcta taatgaacag gcgcttgtag
     2221 cgaagggtct ctcgccgcag acgcttcaga tttactattt caatacagag ctgcagcaat
     2281 acgtggccgt tcccagctat gtggatgacg
//
LOCUS       DAORHF010000411         2266 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_58867_length_2266_cov_3.850294, whole genome shotgun sequence.
ACCESSION   DAORHF010000411 DAORHF010000000
VERSION     DAORHF010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58867_length_2266_cov_3.850294"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2266
                     /locus_tag="PKJ16_19200"
     CDS             <1..>2266
                     /locus_tag="PKJ16_19200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV49173.1"
                     /translation="DTVLDASIKMAYRLFAANSELKKKEALLKESEEKYRLLYEQAGL
                     GIGYYTPEGVVISFNEQALANMGARSEDVVGKSIHDLFPDAEAERYMGRLMQALESDE
                     SAEYEDGVDLPAGSFCFSTVFRRIRDERGSVIGVQLISKNVTERKRAEDALRESERKL
                     HHIEKNIPGILFQARVAPGGSLRFTYVSPRAYDLFGFHLALDDPRWEPGINMHPDDRE
                     RYLQAIASAIASGGDFEFEGRFKPIEGKIPWFQAIATASRIGNELVYNGVIIDITERK
                     LVERELEASERILALINESADLRTLMRGIAAYLYEWSGCEAIGIRLREGEDFPYFEYR
                     GFDEEFIRLENSLCVTDLDGQLLRDDVGNPVIECMCGNVICGRIDPAKPFFTQQGSFW
                     SNCTTELLATTTEADRQAQTRNRCNGMGYESVALIPLRDTGVTFGLLQLNDRRRDRFS
                     PTIIRTLERIADRIAIALAHHHMRAKLEEREERFRLFVDYTYDWEYWIDPDGGFRYVS
                     PSCERITGYSAREFMDDKDLLERIVHADDRSRYRDHIRESRSPDCPACDCTVDFRVVA
                     RTGEVRWIGHRCQPVFGVAGQWLGVRGSNRDITERKNAEQALAGSEERFRTLFEYAPV
                     LIDAFDENGRCILWNKECERVFGWTMDDINAHEDPLALFYPDPEVRREVVSSVTAIPR
                     NSFREWHPVTREGIELTTQWANFMLPDGMVINIGMDFTERRKAEEALAAALEEKTALL
                     AELQHRVKNSLALIS"
BASE COUNT          480 a          655 c          714 g          417 t
ORIGIN      
        1 gataccgttc tggacgcctc gatcaagatg gcctaccgac tcttcgccgc gaacagcgaa
       61 ctgaagaaaa aggaagcgct cctcaaggag agcgaggaga agtatcggct gctgtacgag
      121 caggcggggc tgggcatcgg ctactacacg cccgagggcg ttgtgatctc cttcaatgag
      181 caggctcttg cgaacatggg cgcacggagc gaggatgtcg ttggaaaatc aatccacgat
      241 ctcttccccg atgctgaagc ggaacgctac atgggcaggc tcatgcaagc gctcgaatcc
      301 gacgagagcg ccgagtacga ggacggtgtc gatctgccgg cgggatcgtt ctgcttctcg
      361 accgtctttc ggcgcatacg ggacgagcgc ggaagcgtaa ttggcgtaca gctcatctcg
      421 aagaatgtta ccgagcggaa acgggccgaa gacgccttgc gcgagagcga gcggaagctc
      481 catcacatcg aaaagaacat cccggggata ctgttccagg cgcgggtcgc gccgggaggc
      541 tcccttcgct tcacctatgt cagtccccgg gcatatgacc tgttcggatt ccacctcgcg
      601 ctcgacgatc cccgatggga gcccggcatc aacatgcatc cggacgaccg tgagcgttat
      661 cttcaggcga tcgccagtgc gatcgcgagc ggcggtgatt ttgaatttga gggccggttc
      721 aagccaattg aagggaaaat accctggttt caggcgattg ccaccgcatc ccggatcggc
      781 aatgaactcg tctataatgg cgtcatcatc gacattaccg agcgaaagct cgtcgagcgg
      841 gagctcgagg cctcggagcg catcctcgca ttgatcaacg agagcgcgga cctgcgcacc
      901 ctgatgcggg gcatcgccgc ctacctgtac gaatggtccg gctgcgaggc gatcggcatt
      961 cgcctgagag aaggggagga tttcccgtat ttcgagtacc gcgggttcga cgaggaattc
     1021 attcgtctcg aaaacagcct ctgtgtgacc gacctcgacg ggcagctgct gcgcgacgat
     1081 gtcggcaacc ccgtgatcga gtgcatgtgc ggcaacgtga tctgcgggcg catcgatccc
     1141 gcgaagccgt ttttcacgca gcagggcagc ttctggtcga actgcaccac ggaactcctc
     1201 gcgaccacca ccgaggcgga ccgacaggcg cagacgcgca accgctgcaa tggcatgggc
     1261 tatgaaagcg tggcgctgat tcccctgcgg gatactggcg ttaccttcgg cctgctgcag
     1321 ctgaacgacc ggcgccgtga ccggttcagc ccgacgataa tcaggacgct ggagcgcatc
     1381 gccgaccgga ttgcgatcgc gctggcgcac caccatatgc gcgcaaagct cgaggagcgt
     1441 gaggagcggt tccgcctgtt cgtcgattat acgtacgact gggaatactg gatcgacccg
     1501 gacggcggat tccggtacgt ttccccgtcg tgcgagcgga taaccggcta ttccgcccgg
     1561 gagttcatgg acgacaagga tctcctcgag cggatcgttc atgccgatga tcgctctcgg
     1621 taccgggatc acatccggga gtcacgcagt cccgattgcc ctgcctgcga ctgcaccgtc
     1681 gattttaggg tggtcgcccg gaccggcgag gtgcgatgga tcgggcatcg ctgccagcca
     1741 gtgttcggcg tagcgggaca gtggctcggc gtgcgtggga gcaaccgcga catcaccgag
     1801 cggaaaaacg cggagcaggc gctcgccggg agcgaggagc ggttcaggac gctctttgaa
     1861 tatgctcccg tcttaatcga cgcattcgat gaaaatggcc gctgcatatt atggaacaag
     1921 gaatgcgaaa gggtcttcgg gtggaccatg gacgatatta atgctcatga agaccccctt
     1981 gccctgttct atcccgatcc ggaagtccgt cgagaggtgg tcagtagtgt aaccgccatt
     2041 ccacggaaca gtttccggga atggcatccc gtgaccaggg aaggaataga actgaccacg
     2101 caatgggcga acttcatgct tcccgatgga atggtgatta acatcgggat ggatttcacc
     2161 gaacgcagga aagccgagga ggcgctcgcg gcggcgctcg aggagaagac cgcgctcctt
     2221 gccgagctcc agcaccgcgt gaagaacagc ctcgcgctca tatcga
//
LOCUS       DAORHF010000412         2257 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_59258_length_2257_cov_3.913261, whole genome shotgun sequence.
ACCESSION   DAORHF010000412 DAORHF010000000
VERSION     DAORHF010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59258_length_2257_cov_3.913261"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..1844
                     /locus_tag="PKJ16_19205"
     CDS             168..1844
                     /locus_tag="PKJ16_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HNV49174.1"
                     /translation="MKIVYLTDIHGAFERVKNLLVETVADVYIIAGDLIDIPFYNMDS
                     AIKYHELQTYFHALRRRMEQEGTIIEDFIDELLDMPNVSDEIQEMGSKYQQYTIRARR
                     VMQQKYKIIENIVSTKQSALCLCLPGNYDMDLKYTSLHERDLHLHWYQVEHLKIAGYG
                     GADTWTAGIPERYVVNHQAGIGVDDKKNEMNRFFKAVKPDIIVSHQPAHGVHDRLSFK
                     GPSGSPALRTYCENNPVLLCLTGHIHSDWGFFESDGTVYLNPSNFGEVTTVTGEVSEG
                     GFFHRIEVDAGRVVGVKFRKLVDDRVYDIADYVPENGKWTERVVDAERFEALRRGENF
                     DMKVKKYSHIPEIVLFKEIKQFFRMFQTQESEERVERLEEVVQRIEGKLDDIAMDLVG
                     SVNLGMSQPSSDIDVVLYLRCNAQCSEMLVNCEHFKQAERLIREQLGDDYKFEIIDCI
                     DLTVVERSIREKNYECEVTQRFVTYRSICRPVNYRVIAPLEDLLNDDIEFRMELEGSI
                     RSYFKIFVTTSQHIRSFDKYESRLKSIGIKLPEAIRRKIIHYLQGGPPTA"
     gene            complement(1857..2006)
                     /locus_tag="PKJ16_19210"
     CDS             complement(1857..2006)
                     /locus_tag="PKJ16_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49175.1"
                     /translation="MDITSLVFVTAGALAINGIRIDIRIITDLLLSVSARTGAEHDYH
                     TGTRE"
     gene            2005..>2257
                     /locus_tag="PKJ16_19215"
     CDS             2005..>2257
                     /locus_tag="PKJ16_19215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49176.1"
                     /translation="MNGDVLLQNIFSVFIIAIILEAAVMALFNLSALKGLDRNRAIEA
                     TRDGIIIIVAFFLCWKVDILRIFRGTGMNMPWMVDVIISG"
BASE COUNT          467 a          686 c          689 g          415 t
ORIGIN      
        1 gggcaccgtg agcgccgacc tcgatttctc gatgaaccgg acgaccgtgt acgcccgcat
       61 cggggatgtg tttttatatc tggtcgtcgc gttcatcgcg gccgtcgggg cgttcgccct
      121 gaacgcgtgg cgacgcgagc ggcggggata gggcggacgg gaccggcatg aagatcgtct
      181 atctcaccga catccacggc gccttcgagc gggtgaaaaa cctgttggtc gagactgtgg
      241 ccgacgtcta catcatcgcg ggggacctca tcgacatccc cttctataac atggattccg
      301 ccatcaagta ccatgaactg cagacgtact tccacgccct gcggcgccgc atggaacagg
      361 aagggaccat catcgaggac ttcatcgacg agttgctcga tatgcccaac gtctccgacg
      421 agatccagga gatgggcagc aagtatcagc agtacaccat ccgcgcgcgg cgcgtgatgc
      481 agcagaagta caagatcatc gagaatatcg tgtccaccaa gcagtcggcg ctgtgcctgt
      541 gcctgcccgg caactacgac atggatctca agtacacctc gctgcacgag cgcgacctgc
      601 acctgcactg gtatcaggtc gagcacctga agatcgccgg ctacggcggc gccgacacct
      661 ggaccgccgg tatccccgag cgctatgtcg tgaaccatca ggcgggtatc ggcgtggacg
      721 acaagaagaa cgagatgaac cgcttcttca aggccgtgaa gcccgacatc atcgtgagcc
      781 atcagccggc gcacggcgta cacgaccggc tctcgttcaa gggcccgtcc ggatcgccgg
      841 ccctgcgcac ctactgcgag aacaacccgg tgctgctgtg cctcaccggg cacatccaca
      901 gcgactgggg ttttttcgag agcgacggga ccgtctacct caacccgtcg aacttcggcg
      961 aagtgaccac cgtgaccggg gaggtctccg agggcggctt cttccaccgg atcgaggtcg
     1021 acgccgggcg ggtggtcggc gtcaagttcc gcaagctcgt ggatgatcgc gtctacgaca
     1081 tcgccgacta cgtcccggaa aacgggaagt ggaccgagcg ggtggtcgac gccgagcgct
     1141 tcgaggcgct gcgccgcggc gagaacttcg acatgaaggt gaagaagtat tcgcacatcc
     1201 cggagatcgt gctgttcaag gagatcaagc agttcttccg catgttccag acgcaggagt
     1261 ccgaggagcg cgtggagcgg ctcgaggagg tggtgcagcg catcgagggc aagctcgacg
     1321 acatcgcgat ggacctggtg ggctcggtga acctggggat gtcgcagccg agctccgaca
     1381 tcgacgtggt gctgtacctg cgctgcaacg cgcagtgctc cgagatgctg gtcaactgcg
     1441 agcacttcaa gcaggccgag cgcctcatcc gcgagcagct cggcgacgac tacaagttcg
     1501 agatcatcga ctgcatcgac ctgacggtgg tggagcgcag catccgcgag aagaactacg
     1561 agtgcgaggt cactcagcgc ttcgtgacct accgctccat ctgccgcccg gtgaactatc
     1621 gcgtcatcgc cccgctcgag gacctgctca acgacgacat cgagttccgc atggagctgg
     1681 aggggagcat ccgctcgtat ttcaagatct tcgtgactac ctcgcagcac atccgctcgt
     1741 tcgacaagta cgaatcgcgc ctcaagtcca tcggcatcaa gctcccggag gcgatccgcc
     1801 ggaagatcat ccattacctg cagggcggcc cgccgacggc ataaaaaacc ttgattttat
     1861 tcacgggtgc cggtatgata atcatgttcg gcgcccgtcc gcgccgatac tgagaggagg
     1921 agatcggtta tgatacggat gtcgattctg atgccattga tcgccaaggc gcccgccgtg
     1981 acaaaaacaa gcgaggtgat gtccatgaac ggtgacgtac tgctccaaaa catcttcagc
     2041 gttttcatta tcgccatcat cctggaggcg gccgtgatgg cgctgttcaa tctctcggcg
     2101 ctcaagggat tggaccgcaa ccgcgcgatc gaggcgacgc gcgacgggat catcatcatc
     2161 gtcgcgttct ttctctgctg gaaggtggac atccttcgca tcttccgcgg gaccggcatg
     2221 aacatgccgt ggatggtcga cgtcatcatc agcggcc
//
LOCUS       DAORHF010000413         2256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_59304_length_2256_cov_3.677419, whole genome shotgun sequence.
ACCESSION   DAORHF010000413 DAORHF010000000
VERSION     DAORHF010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59304_length_2256_cov_3.677419"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /gene="msrA"
                     /locus_tag="PKJ16_19220"
     CDS             <1..498
                     /gene="msrA"
                     /locus_tag="PKJ16_19220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HNV49177.1"
                     /translation="SDTNAVPARYEKATFAAGCFWGVEHTLGTMKGVKATSVGYIGGR
                     TANPTYEQVCSGATGHAEAVEVTFDPSVVSYNELLAAFFALHNPTTLNRQGPDVGEQY
                     RSAIFYHTDEQKKAAEAMIRTLTTEKRFKQPIVTQVVPAGTFFRAEEYHQKYHEKRGG
                     GSCAF"
     gene            763..>2256
                     /locus_tag="PKJ16_19225"
     CDS             763..>2256
                     /locus_tag="PKJ16_19225"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNV49178.1"
                     /translation="MNKKALIGMIAFIVVYFIVQIVSGFYVDWEWFRINGHLNVFWTL
                     LLTKFNVHGIFSAAFIALFFLNFLLIRLMAGSGRIFTSAILNRLQLPFLGTPRRALFI
                     VIAAGVVVTGFFLGLAASVYWREFLIYLNAVPFEGFPKDPIFSLDMGFYVFRLPFYQF
                     LYGWLMFCLAIITVFSLVFHLANGGIGYREGAFNFSLFARAHLSTLAALIVILYGVGY
                     RLSAYQLLFADKGRFFGAGYTEANAQLLAYNVCMVISFVAAGLLLFNIVKRSFKLPVL
                     VLLTLIPVNFVLGTVYPGLMQRFVVVPNELDKETKYIEHNIAFTRRAYGLEGMAERPF
                     ANKPTLTPADIARNRSTLESIRLWDWRPLKQTYKQLQELRPYYQFNDVDVDRYSINNT
                     LLAVNLAARELAVANIAKSSQTWVNKHLVYTHGYGIVFNRVDRVTADGQPEMLIYDIP
                     PKTKIPLEVKRPEIYYGEHDNPYVITNTTIQPGEFDYPYGDQNRYTRY"
BASE COUNT          409 a          760 c          651 g          436 t
ORIGIN      
        1 tcggatacaa acgccgtccc cgcacggtac gagaaggcga ccttcgccgc cgggtgcttc
       61 tggggcgtcg agcatacgct tggcacgatg aagggcgtga aggcgacgag cgtcggctac
      121 atcggcggcc ggaccgcaaa ccccacctac gagcaggtgt gctcgggcgc gacgggacat
      181 gcggaggcgg tggaggtcac gttcgatccg tcggtggtga gctacaacga gctcctcgcg
      241 gccttcttcg cgctgcacaa cccgaccacg ctcaaccggc agggcccgga cgtgggcgag
      301 cagtatcgct cggccatctt ctatcacacc gacgaacaga agaaggccgc cgaggcgatg
      361 atacgaacgc tcaccacgga gaagcgattc aagcagccca tcgtcacgca ggttgttccc
      421 gccgggacgt tttttcgcgc ggaggagtat catcagaaat atcacgagaa acgcggcggg
      481 ggcagttgcg cgttttgacg ctccgacccc ctccgccctt tgggcacctc ccccttcgtt
      541 agggggaggc agaagcagga gctcgctgcc acatcatccc cgttttttta cttgcaaata
      601 atgcgctcgc tgatctgcta ctatcacaac agcgtgcgac cgaccgtgcg gcggtttcgc
      661 cagcgacaca cattcgccgg ccacacggcg gtcccggcat agcgcaccga tcgcggatct
      721 tgcgggacgg ccgcgcgagc gtcatcaccc aggagcgtta ccatgaacaa aaaggccctc
      781 atcggaatga tcgccttcat cgtcgtctat ttcattgtcc agatcgtctc cggcttttac
      841 gtggactggg agtggtttcg catcaacggg catctcaacg ttttctggac gctgctgctg
      901 acgaagttca acgtgcacgg aattttctcc gcggccttca tcgcgctttt ctttctcaac
      961 ttccttctca ttcggctgat ggcgggctcg ggccggatct tcaccagcgc catcctcaat
     1021 cggctgcagc tgccgttcct gggaacgccg cggagggcgc tgttcatcgt catcgcggcg
     1081 ggagtggtgg tcacgggatt tttcctgggg ctggcggcgt cggtctactg gcgcgaattt
     1141 ctcatctacc tgaacgcggt ccccttcgag ggctttccga aggacccgat cttctccctc
     1201 gacatgggct tctacgtctt ccggctcccg ttctatcagt tcctctacgg gtggctcatg
     1261 ttctgcctgg ccatcatcac cgtcttctcg ctggtgttcc acctggccaa cggcgggatc
     1321 ggctaccgcg agggcgcgtt caacttctcg ctcttcgcgc gggcgcatct ctcgacgctg
     1381 gccgcgctca tcgtgatcct ttacggcgtc ggctaccgcc tgtcggccta ccagctcctg
     1441 ttcgcggaca aggggcgctt cttcggcgcg ggctacactg aggcgaacgc gcagctccta
     1501 gcgtacaacg tgtgcatggt catctccttt gtcgctgcgg ggctgctgct gttcaacatt
     1561 gtcaagcgga gcttcaagct cccggtgctc gtgctcctca cgctcatccc ggtcaatttc
     1621 gtgctcggga cggtctaccc gggcctcatg cagcgcttcg tcgtggtccc caacgagctc
     1681 gacaaggaga cgaagtacat cgagcacaac atcgccttca cccggcgcgc ctacggcctc
     1741 gagggcatgg ccgagcgccc cttcgccaac aagcccacgc tcaccccggc cgatatcgcg
     1801 cgcaaccgct ccacgctgga gagcatccgc ctgtgggact ggcgcccgct caagcagacc
     1861 tacaagcagc tccaggagct gcggccctat taccagttca acgacgtcga cgtcgaccgc
     1921 tacagcatca acaacaccct gctcgcggtg aacctcgcgg cgcgcgagct ggccgtcgcg
     1981 aacatcgcca agagcagcca gacctgggtg aacaagcacc tggtgtacac gcacgggtac
     2041 gggatcgtct tcaaccgcgt ggaccgcgtg accgccgacg gccagccgga gatgctcatc
     2101 tacgacatcc cgcccaagac gaagatcccg ctcgaggtga aacggcccga gatctactac
     2161 ggggagcacg acaacccgta cgtgatcacc aacacgacca tccagccggg cgagttcgac
     2221 tacccctacg gcgaccagaa ccgctacacg cgctac
//
LOCUS       DAORHF010000414         2194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_61948_length_2194_cov_3.900421, whole genome shotgun sequence.
ACCESSION   DAORHF010000414 DAORHF010000000
VERSION     DAORHF010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61948_length_2194_cov_3.900421"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2194)
                     /locus_tag="PKJ16_19230"
     CDS             complement(<1..>2194)
                     /locus_tag="PKJ16_19230"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HNV49179.1"
                     /translation="VGKQGGKTVALNSATCTTVASIPYPIKVEGEPGKYLTSSYSDPK
                     DVQYLKDLSQKIIGDAKTLDAAMARIGYWVRNHVSYELGAPTDAIGVIKARRAFCVGY
                     GEVMTELLRAAGIPARTLTCYVPPGCGWGFNDSGGMHAFVEFYYPGKGWLCSDPQSTL
                     DYVDPFHIIESAYVSTIASDNRNYVVDYQEEPKNWPAFKAMSRGRYPRPVIVLSVRTR
                     DGSYIYNAERASFKVFKPAVFGSYQTVGDVRGFKATFYSNSIFSFMQSGGRYASIIIT
                     DPEFIAKRYTETISVASDSCLFVKDVNLTGPGKYMVEGDFYENPNVRRIVVRGENDAP
                     VSEKTVTVTVNGTPYDISTCKNGLVHVLANGNVSALTVDGVTHALAFDKNLVCRVPGK
                     RPDGDAVGAFIRERKIDQAAGTHLLAACYDIAGALSVNAFDGVYLCSRGMEPKRLRIE
                     APGIAQCGGMEIGRDYWLLFERGNRYIRKNVRFTANGIRVLDAKPLEETYTLLEGALS
                     KYLPMKAFYEYYDGMKTIVYTANRGRVYLSLPEGEYLFSDNDKGTDVQRIRFDRRELK
                     RVYSPESQTLDEFIAVSRMLHPAGTMIAGSVIADGAKAAGGSIMLFNEERRSTVTATV
                     NASGHYLFSGVDPGARNRLIYSGGGLFAAKTVIPQKGLVTKAVIDTAHGKSSTLKTYT
                     VRRETHGSRTFHYRSAGSRALFLLVPYAAKGRPDVVAVPLSNGDETRLC"
BASE COUNT          440 a          676 c          604 g          474 t
ORIGIN      
        1 ggcagagtct cgtttcatcg ccattcgaca acgggaccgc gacgacgtcg ggccgcccct
       61 tcgccgcata gggcaccagc agaaagagcg ccctcgagcc tgcacttcga tagtggaacg
      121 tcctggagcc gtgcgtctct ctcctgaccg tatacgtctt gagagtggac gatttcccgt
      181 gcgccgtatc gatcaccgct ttcgtcacga gtcccttctg cggaatgacc gtcttggcgg
      241 cgaaaagtcc cccgcccgaa tagatgagtc gattgcgcgc gcccggatcg acgccggaga
      301 acaggtaatg cccggatgcg ttgacggtcg ctgtgaccgt actccggcgc tcctcattga
      361 acagcataat cgagccgccg gctgccttcg cgccgtccgc gataaccgaa cccgcgatca
      421 tcgtccctgc gggatgcagc atcctgctca cggcgatgaa ctcgtcaagc gtctgcgact
      481 ccggcgaata cacccgcttc agctcgcgcc ggtcgaagcg gatccgctgg acgtccgttc
      541 ccttgtcatt atcggagaaa agatactcgc cctcgggcag gctgagatac accctgcccc
      601 tgttcgccgt atatacgatc gtcttcattc cgtcgtaata ctcgtaaaac gccttcatcg
      661 gaagatattt ggacaaagcg ccttcaagaa gcgtataagt ttcttccaac ggctttgcgt
      721 ctagtacccg gataccgttc gcggtaaacc gcacgttctt tctgatatat cggtttccgc
      781 gctcgaagag aagccagtag tctctgccga tttccatccc cccgcactgc gcaatccccg
      841 gggcctcaat cctgagccgc ttcggctcca tgccgcgtga gcagaggtat acgccatcga
      901 aggcgtttac ggaaagagcg cccgcgatgt catagcacgc ggccaggaga tgcgttcccg
      961 ccgcctggtc gatcttcctc tcgcggataa atgctcctac cgcatcgccg tccggccgct
     1021 tcccgggaac ccggcacacc aggttcttgt cgaacgcaag tgcgtgggta acgccgtcca
     1081 ccgtgagtgc gctcacgttc ccgttggcga gaacatggac gagcccgttc ttacatgtcg
     1141 agatatcgta gggggttccg ttgacggtaa ccgttacggt cttctcagag accggcgcgt
     1201 cattttcgcc tcgaacgacg atccgtctga cgttcggatt ctcgtagaaa tcgccctcga
     1261 ccatgtactt gccggggccc gtgagattga cgtccttcac gaacaggcag gagtcggaag
     1321 cgaccgagat ggtttcggtg taccgcttcg cgataaactc agggtcggta atgatgatgc
     1381 tcgcatagcg gccgccggat tgcataaaac tgaatatact gttggagtag aaggtcgctt
     1441 tgaacccacg cacatcgccc accgtttgat atgatccgaa taccgccggt ttgaagacct
     1501 tgaacgatgc ccgctcggcg ttgtagatat atgagccatc acgcgtgcgc acgctgagga
     1561 caatgacggg acgcgggtat ctcccacgcg acatggcttt gaacgccggc cagttcttag
     1621 gctcttcctg ataatcgacg acatagttgc gattgtccga tgcgatcgtc gatacatacg
     1681 ccgactcgat tatgtgaaag ggatctacgt aatccagggt cgattgcggg tcggagcaga
     1741 gccatccctt accagggtaa taaaattcga cgaacgcatg catgcccccg gaatcattga
     1801 atccccatcc gcatcccggg gggacatagc acgtgagcgt tcgcgccggg atgcctgcgg
     1861 cccgcagaag ctctgtcatg acctcgccat agcccacgca gaaagcccgc cgcgccttga
     1921 tgacgccaat cgcatcggtt ggtgcgccaa gctcgtaact tacatgattg cgcacccagt
     1981 agccgatacg ggccatcgcc gcatccagcg ttttcgcgtc gccgatgatt ttttgcgaaa
     2041 ggtctttcaa atactgcacg tccttgggat cgctataact gctggtcagg tatttccccg
     2101 gctccccttc gaccttgatg ggatagggga tcgaagcgac cgtcgtgcag gtggcggaat
     2161 tgagcgcgac ggtcttgccc ccctgcttcc cgac
//
LOCUS       DAORHF010000415         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_62737_length_2176_cov_4.031117, whole genome shotgun sequence.
ACCESSION   DAORHF010000415 DAORHF010000000
VERSION     DAORHF010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62737_length_2176_cov_4.031117"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /gene="lon"
                     /locus_tag="PKJ16_19235"
     CDS             <1..1577
                     /gene="lon"
                     /locus_tag="PKJ16_19235"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HNV49180.1"
                     /translation="TEIEKFEERIKNLKLTDEAATKVREEIEKIKVLEPHSAEYGVSR
                     NYPEWLTTLPWGVHSADNYDIAKARSVLDRDHYGLKDIKERILEFISTGKMKGNITGS
                     IICFVGPPGVGKTSIGKSVAEALGRKFFRFSLGGMRDEAEIKGHRRTYIGAMPGKIIQ
                     SLKTVGTANPVIMLDEIDKIGISFQGDPASALLEVLDPEQNSQFLDHYIDVRFDLSNI
                     LFIATANQMDTIPLPLLDRMEIMKLSGYILEEKMQIAKKYLIPKQLKEHGLTKAMASI
                     DMAALKRIIEGYAREAGVRSTENNIKKIMRKAAKKIAEGEATAVAITEKNLSDFLGKP
                     VFTDEELYDKRIPGVVMGLAWTSMGGATLYVEATAIPSKSRGFKQTGQLGDVMKESTE
                     IAYTYVSSKVKEFGIPEDFFDTRAIHLHVPAGATPKDGPSAGITMATALYSLAKGKPI
                     KKNIAMTGELTVTGKVLPIGGVKEKTLAAKRAKVKTIIYPKENKRDFDELPDHIRKGV
                     DARFVNYFDDVLKIVY"
     gene            complement(1880..>2176)
                     /locus_tag="PKJ16_19240"
     CDS             complement(1880..>2176)
                     /locus_tag="PKJ16_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49181.1"
                     /translation="LLSLVQRAALRHSRWHPELREHPSVENKPADKYEYNPDPGVHHF
                     LLAGRSAVDHEAGTVLRVHEVDLHGRTLAQIAGKLGVDDYIDRIVRDPRFVGLR"
BASE COUNT          545 a          624 c          630 g          377 t
ORIGIN      
        1 ccaccgagat cgagaagttc gaggagcgca tcaagaacct gaagctcacc gacgaggccg
       61 ccacgaaggt ccgcgaggag atcgagaaga ttaaagttct cgaaccgcac tcggcggagt
      121 acggcgtgag ccgcaactac ccggaatggc ttaccacgtt gccgtggggc gtgcactcgg
      181 cggacaacta cgacatcgcc aaggcacgct cggtcctcga ccgcgaccac tatgggctca
      241 aggacatcaa ggagcgcatc ctggagttca tcagcaccgg gaaaatgaag ggaaacatca
      301 ccggctccat catctgcttc gtggggcctc ccggcgtggg taagacctcc atcggaaaat
      361 cggtcgcgga ggcgctggga cgcaagttct tccgcttctc gctgggcggc atgcgcgacg
      421 aggccgagat caagggacac cggcgcacct acatcggcgc gatgccggga aagatcatcc
      481 agagcctcaa gaccgttggc accgccaacc ccgtcatcat gctcgacgag atcgacaaga
      541 tcggcatcag cttccagggc gatccggcct ccgcgctcct tgaggtgctg gaccccgagc
      601 agaattcgca gttcctggac cactacatcg acgtccgctt cgacctgtcg aacatcctct
      661 tcatcgcgac cgccaaccag atggacacca tccccctgcc cctcttggac cgcatggaga
      721 tcatgaagct ctccggctac atcctcgagg agaagatgca aatcgcgaag aaatacctca
      781 tccccaagca gctcaaggag cacggcctca cgaaggcgat ggcgagcatc gacatggccg
      841 cgctcaagcg gatcatcgag ggctatgcgc gcgaggccgg cgtccgcagc accgagaaca
      901 acatcaagaa gatcatgcgg aaggccgcca agaagatcgc cgagggagaa gcgacggccg
      961 tcgccatcac cgagaaaaac ctgtccgatt ttctcggcaa gcccgtcttt accgacgagg
     1021 agctctacga caagcgcatc cccggcgtgg tgatggggct cgcgtggacg agcatgggcg
     1081 gcgcgacgct gtacgtagag gcgaccgcga tcccgtcgaa gtcgcgcggc ttcaagcaga
     1141 ccgggcagct gggcgatgtg atgaaggaat ccaccgagat cgcctacacc tacgtgagtt
     1201 ccaaggtgaa ggaattcggg attccggaag attttttcga tacgcgcgcg atccatctgc
     1261 acgtccccgc cggcgcgacg cccaaggacg ggccctcggc cgggatcacc atggccacgg
     1321 cgctctactc gctggcgaag ggcaagccta tcaagaagaa catcgcgatg acgggcgagc
     1381 tcaccgtgac cggcaaggtg ctgcccatcg gcggcgtgaa ggaaaagacg ctcgcggcca
     1441 aacgcgccaa ggtgaagacg atcatctacc cgaaagagaa caagcgggac ttcgacgagc
     1501 tgcccgacca catccgcaag ggtgtcgatg cgcgcttcgt gaactacttc gacgatgtgc
     1561 tgaaaatcgt ctactgagaa ccgattcaag aagggaaccg aaagagcctg ctggaataaa
     1621 cgctatgagc ctattccatt ggggaattta aaaattctaa aaaatacaaa aaagaagttg
     1681 acgattctat tgtattttag tattttattt aatattaaat actaatggag agactgctat
     1741 gaaaatatca atcacagata gcgccagaga caagctcaag gagataatag cgtcaagcga
     1801 actgccccat ccatcattgc gacttgtcgt cgcaggagtc ggttgaggcg ggccccggct
     1861 ggggctgact ctggatgagt catcgaagac cgacgaaacg cggatcacgg acaatacgat
     1921 cgatataatc gtcgacaccg agcttacccg ctatctgcgc cagggttcgg ccgtgaagat
     1981 cgacttcatg gacacgcagt acggttccgg cttcatgatc gacagcggat cgacctgcta
     2041 ggaggaaatg atggactcct ggatctggat tatactcgta cttgtcggct ggtttgttct
     2101 caacagatgg gtgctcccga agctcggggt gccaacgtga atgtcgcaaa gctgctctct
     2161 ggacgagaga aagaag
//
LOCUS       DAORHF010000416         2166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_63176_length_2166_cov_4.938892, whole genome shotgun sequence.
ACCESSION   DAORHF010000416 DAORHF010000000
VERSION     DAORHF010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63176_length_2166_cov_4.938892"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            197..640
                     /locus_tag="PKJ16_19245"
     CDS             197..640
                     /locus_tag="PKJ16_19245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49182.1"
                     /translation="MDGKKLKAQILQDLLAENKKFLYEKVRSARIEVRGRSLALLYPQ
                     DSGESYYRRMLDANDLAHISELAGKRTGATVSVSVGDDAAVPPPADELVPDEALPDDA
                     EMVRAPEVDEMDELNPMVEKIKDMFHGQIVESKQKKEKNNAQRPR"
     gene            621..944
                     /locus_tag="PKJ16_19250"
     CDS             621..944
                     /locus_tag="PKJ16_19250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HNV49183.1"
                     /translation="MLKGLGDVGQMIKLQRELKNIQKRLKKSEASGTSHDGAVSVTVN
                     GEYRIVDLSIDQEFAGKADKKTLEKAIANAVNNAVDRIKEHMAEEMQKLTGGMNIPGM
                     GDLLK"
     gene            958..1548
                     /gene="recR"
                     /locus_tag="PKJ16_19255"
     CDS             958..1548
                     /gene="recR"
                     /locus_tag="PKJ16_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HNV49184.1"
                     /translation="MSRYLDALVREFSRLPGVGPKSASRLAFHVLRMPEGDVRSLVDA
                     IREVREHITACAVCGGIADGERCAICADPARDRGVICVVEQQKDVLTVERSGAFTGLY
                     HVLNGVISPLDGIGPEELNIKPLLERCKDAAVVEIVIATNPSVEGDATSLYLSKIIKP
                     SGIKVTRIAHGIPVGADLEFADGATIYQSLKCRVEM"
     gene            1575..2126
                     /locus_tag="PKJ16_19260"
     CDS             1575..2126
                     /locus_tag="PKJ16_19260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49185.1"
                     /translation="MNRIACCALIVLASCLVSASAAPVHTQCGPDVSFSFDDAVWQIA
                     RPAAPEAGKKSYELLLRLKANPGEQARFWSVQLGAEERAKMEAMSPAQLLGHMANMLS
                     RRNTGPAITATESAGPVHFAYVESQGPSGRPFSLYVGAVRKGDLLLYVMHMFEPHRSE
                     GTALDARSMKARFVALVSTIAVR"
BASE COUNT          426 a          657 c          728 g          355 t
ORIGIN      
        1 ccgatctggc cctgatcacg gcgcacctgc aacgccgcga gttcgatcag gcactacggt
       61 ccttcccgaa gcgaagccat cgacgcctcg cagtgaatcc gcatcgaaaa ccgctccggg
      121 aaacgtgccg atcgcgccgt ctggcgagca gccggcgccg acgcaacagg atactcccgt
      181 cccggaggac gccggggtgg acggcaagaa gctcaaggcg cagatactgc aggacctgct
      241 ggcggagaac aagaaattcc tctacgagaa ggtgcgatcc gcgcgcatcg aggtgcgcgg
      301 ccgctccctc gcgctgctgt acccgcagga ttccggcgaa tcgtactacc gaagaatgct
      361 tgacgcgaac gatctggcgc atatctcgga gctcgccggg aagcgcaccg gcgcgaccgt
      421 atcggtgtcg gtgggcgacg acgcggctgt accgcccccc gcggacgagc tggttcccga
      481 cgaggcgctg ccggacgacg ccgaaatggt acgcgcgcct gaggtggacg agatggacga
      541 gctcaatccc atggttgaga aaatcaagga catgtttcac gggcaaatcg tagaatcgaa
      601 acaaaagaag gagaagaaca atgctcaaag gcctcggtga tgtcggtcag atgatcaagc
      661 tccagcggga gctcaagaat atccaaaaac gactcaagaa gtcggaggcg tccgggacga
      721 gccacgacgg cgcggtttcg gtcaccgtga acggcgagta ccgcatcgtc gatctctcga
      781 tcgaccagga gttcgcgggg aaggccgaca aaaagacgct ggagaaggcg atcgcgaacg
      841 ccgtgaacaa cgcggtcgat cgcatcaagg agcacatggc cgaagagatg cagaaactca
      901 ccggcggcat gaacatcccg ggcatgggcg acttgctgaa atagaatagt gggcgctatc
      961 tcgcgatatc ttgacgccct ggtccgtgag ttctcgcggt tgccgggggt cggacccaag
     1021 agcgcctcgc gtctcgcctt ccatgtgctt cggatgccgg aaggcgacgt gcgctcgctg
     1081 gtggacgcga tacgcgaggt gcgcgagcat atcaccgcct gcgccgtgtg cgggggcatc
     1141 gccgacggcg agcgctgcgc catctgcgcc gatccggcgc gcgaccgggg cgtcatctgc
     1201 gtcgtcgaac agcagaagga cgtgctcacc gtggagcgct cgggcgcgtt cacggggttg
     1261 tatcacgtgc tcaacggcgt catctccccg ctcgacggga tcggcccgga ggagctcaac
     1321 atcaaaccgc tcctggagcg ctgcaaggac gcggcggtcg tcgagatcgt catcgcgacc
     1381 aaccccagcg tagagggcga cgcgacgagc ctgtatcttt ccaagatcat caagccgtcg
     1441 gggatcaagg tcacgcgcat cgcccacggg atccccgtgg gggccgacct cgagttcgcc
     1501 gacggcgcca cgatatatca atcgctcaag tgccgcgtgg agatgtgacg cggcggcgga
     1561 aggaggccat ccccatgaat cgaatcgcgt gttgcgccct gatcgttctg gcgagttgcc
     1621 tggtgtcggc gtccgcggcg ccggtgcaca cgcaatgcgg tcccgacgtt tcgttttcat
     1681 tcgatgacgc cgtatggcag atcgcgcgac cggccgcgcc cgaggcgggg aaaaaatcct
     1741 atgagctgtt gcttcgcctt aaggcgaatc ccggtgagca ggcgcgcttc tggtcggtgc
     1801 agcttggcgc ggaggagcgc gcgaagatgg aggcgatgtc gccggcgcag cttctcgggc
     1861 acatggcgaa tatgctgagc cgccgcaata ccggaccggc aatcaccgcg acggaatcgg
     1921 ccggtcccgt tcattttgcc tatgtcgaat cgcagggccc gagcgggcgg ccgttttcgc
     1981 tgtatgtggg cgccgtgcga aagggcgatc tcctgctcta tgtgatgcac atgttcgagc
     2041 cgcaccgctc cgaagggacg gcgctcgatg cgcggtcaat gaaggcgcgc tttgtggcgc
     2101 ttgtttcgac gatcgcggtg cggtagcgcc gtcgtcccgt cgcccggcgg gctaacggtc
     2161 aggagg
//
LOCUS       DAORHF010000417         2165 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_63225_length_2165_cov_4.887678, whole genome shotgun sequence.
ACCESSION   DAORHF010000417 DAORHF010000000
VERSION     DAORHF010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63225_length_2165_cov_4.887678"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="PKJ16_19265"
     CDS             <1..698
                     /locus_tag="PKJ16_19265"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HNV49186.1"
                     /translation="LTVVGVFRSGNPEYDQTLLVMPIEQARATFSMERYIHAIVVRLA
                     DIKHDAAVIDSLRASLPETERSSIEVLGWDTLMPELVQFIALDDISAYIFDFILFLVV
                     AFGILNTVQMAVFERTREFGVMLAVGTRPGQVVHMVLIESAIISTLGVIAGGIAGFLA
                     SLYFKLRPLDFSDYSNEMATIGVSMTKLPADATAANIAVTAVITLALTIVFAYFPARR
                     ASKLKPVEAIRQL"
     gene            712..1947
                     /locus_tag="PKJ16_19270"
     CDS             712..1947
                     /locus_tag="PKJ16_19270"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HNV49187.1"
                     /translation="MQTIFAMGWRNIWRHRRRSLVVISSIALGIFAMILSMGIMNGMN
                     NQAIENTIRTALGHIAIHRKGFHEDMKIAHNFKSTDRIMRSIAADRRIAAAAPRVKLP
                     GMVRSSETSQGVLIVGIDPEKEMKISNLYQYTSKENGSRFLASPDENAALISQQTADR
                     LKLVVGDKLVLMLQDRNNEIVGVGMTVAGTFQTPVESFDKLVVFTGIGKLQEITGLGE
                     NISEISIAITDPAAITDIKRRLIGAIGDDSLEVLTWMDMAPYLVSAVKFFDTIMYITF
                     VIIFLVVVFSVANTLVMAIMERFHEIGVMKSIGTRPSWIATMIVFEAVNLGLVGLVAG
                     VAAGTLSVAALSIGGINLGLFMESLRAWGTGSVIFPVIKGMDIVVAALIVLATTVLAA
                     VYPAVKAARIKPLEALHFV"
     gene            1976..>2165
                     /locus_tag="PKJ16_19275"
     CDS             1976..>2165
                     /locus_tag="PKJ16_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002701298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HNV49188.1"
                     /translation="MNIIELQSITKTYDTNGLTVPAVRGVDLVVEKGEFSALAGPSGS
                     GKTTLLNLIGGLDNPSGGT"
BASE COUNT          403 a          689 c          675 g          398 t
ORIGIN      
        1 agctcacggt ggtgggcgtc ttccgctcgg gcaatcccga gtacgaccag acgctcctcg
       61 tcatgccgat cgagcaggcg cgcgcgacct tctcgatgga gcgctacatc cacgccatcg
      121 tcgtccgtct cgccgacata aagcacgatg cggcggtcat cgattcgctg cgtgcgtccc
      181 tgccggagac ggagcggtcc tcgatcgagg tgctgggatg ggacacgctg atgcccgagc
      241 tcgtccagtt catcgcgctc gacgacatca gcgcctacat cttcgatttc attctgttcc
      301 tggtggtcgc cttcggcatc ctgaataccg tacagatggc ggtcttcgag cgcacgcgcg
      361 agttcggggt gatgctggcg gtgggcacgc ggccgggcca ggtggtccac atggtgctca
      421 tcgaatccgc gatcatatcg acgttaggcg tcattgccgg cggcatcgcg gggttcctgg
      481 cgagcctgta cttcaaactg cggccgctgg atttttcgga ctactcgaac gagatggcga
      541 cgatcggcgt gagcatgaca aaactcccgg ccgatgcgac ggcggcgaac atcgccgtga
      601 ccgccgtcat caccctggcg ctgacgatcg tcttcgccta tttcccggcg cgtcgcgcgt
      661 cgaaactcaa gccggtcgaa gcgatccggc aactgtagcg ggaggataca catgcagacc
      721 atattcgcca tgggctggcg caacatctgg cgacaccgcc gccgctccct ggtggtgatc
      781 tcgtcgatcg ccctgggcat tttcgccatg atattgtcga tgggcatcat gaacggcatg
      841 aacaaccagg cgatcgagaa caccatccgg accgcgctgg gacacatcgc catccaccgg
      901 aagggattcc acgaggacat gaagatcgcg cacaacttca aatcgaccga tcgcatcatg
      961 cggtcgatcg cggccgaccg gcgcatcgcc gccgccgcgc ctcgggtgaa gctccccggc
     1021 atggtccgct cgagcgagac ctcgcagggc gtcctcatcg tgggcattga tcccgagaaa
     1081 gaaatgaaga tctcgaacct gtaccagtac acgtcgaagg aaaacggcag ccgcttcctc
     1141 gcctcgccgg acgagaacgc ggcgctcatc tcgcagcaga cggccgatcg cctcaagctc
     1201 gtcgtgggcg acaagctggt gctgatgctc caggaccgca acaacgagat cgtgggcgtg
     1261 ggcatgaccg tcgccgggac cttccagacg cccgtggaga gcttcgacaa gctggtggtc
     1321 ttcacgggga tcggaaagct gcaggagatc acggggctcg gggagaacat ctccgagatc
     1381 tccatcgcga tcacggaccc cgcggcgatc acggacatca agcggcgcct catcggcgcg
     1441 atcggcgacg attcgctcga ggtgctcacc tggatggaca tggcgcccta cctggtgagc
     1501 gcggtgaagt tcttcgacac gatcatgtac atcaccttcg tcatcatctt cctggtggtc
     1561 gtcttctcag tggccaacac cctggtgatg gcgatcatgg agcgattcca cgagatcggc
     1621 gtcatgaagt cgatcggcac gcgcccgtcg tggatcgcaa cgatgatcgt cttcgaggcg
     1681 gtgaacctgg gcctcgtggg gctcgtcgcc ggtgtcgccg cgggaacgct ctcggtcgcg
     1741 gcgctctcga tcggcgggat caacctgggg cttttcatgg agtcgctgcg cgcctggggg
     1801 accggcagcg tcatcttccc ggtcatcaag ggcatggaca tcgtcgtggc ggccctgatc
     1861 gtcctcgcga ctacggtcct cgcggcggtc tacccggccg tcaaggccgc gcgcatcaag
     1921 ccgctcgagg cgcttcattt cgtataacgc aatagaccat catgtggaga aaacgatgaa
     1981 tatcatagaa ctgcaatcga tcaccaagac ctacgacacc aacgggctca ccgtcccggc
     2041 ggtgcgcggc gtcgatctcg tcgtggaaaa gggcgagttc tccgccctcg cggggccctc
     2101 gggctccggc aagacgacgc tcctgaacct catcggcggc ctggacaatc cgtcgggcgg
     2161 aacgg
//
LOCUS       DAORHF010000418         2128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_65087_length_2128_cov_4.098890, whole genome shotgun sequence.
ACCESSION   DAORHF010000418 DAORHF010000000
VERSION     DAORHF010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65087_length_2128_cov_4.098890"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PKJ16_19280"
     CDS             <1..216
                     /locus_tag="PKJ16_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HNV49189.1"
                     /translation="YLNNSIKAVKVNGIEPTLKNAKAKKNRYPIARELYMYVNENKYT
                     PQAKAFVKFLLGKKGQELVKSAGYIPL"
     gene            complement(217..408)
                     /locus_tag="PKJ16_19285"
     CDS             complement(217..408)
                     /locus_tag="PKJ16_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49190.1"
                     /translation="MKKSAHWKVLLSTHRSRYEHFLELRMELAPYEATILEMIGGART
                     KKWKGARPFHGDESRMRGK"
     gene            complement(593..1018)
                     /locus_tag="PKJ16_19290"
     CDS             complement(593..1018)
                     /locus_tag="PKJ16_19290"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HNV49191.1"
                     /translation="MRLYIDTSALLKRYVSEEGSDRLDAIMGTADEIVVSAITELELC
                     SALRRLLANRVVNADGYRTVLNEFGTDYPSFVCVPFDDVVADNAKALIDAHQLRTLDS
                     IQLGAALAVRDEIDCFVACDAKLATAARKAGLKVFNPLS"
     gene            complement(1015..1239)
                     /locus_tag="PKJ16_19295"
     CDS             complement(1015..1239)
                     /locus_tag="PKJ16_19295"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HNV49192.1"
                     /translation="MQYLKFTDFRNRSKEYFDKVERGSSFIIIRKGKPVAKIVPFGET
                     GAAWKREPKRITLKTGTTTLDYIMHERNER"
     gene            complement(1411..>2128)
                     /locus_tag="PKJ16_19300"
     CDS             complement(1411..>2128)
                     /locus_tag="PKJ16_19300"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HNV49193.1"
                     /translation="QTTLLPERPELSGYEITAYMEPAAEVGGDYYDVISVGGNDWIVI
                     GDVSGHGVPAGLIMMMVQTSIHVALDQNRDLDPSGLLTVINRTITENVRKLNDDKYMT
                     ITVLAAHKDGRFVFAGLHQDILVYRASSAAVEVIETDGVWIGLMEDISGMIGNNSFTL
                     DSGDVLLVYTDGITEAWSRGSRPGTRTPERDMFGAHRLSETLAALGARPTEEIRDGIL
                     QSLRQFECMDDVTMVVIRRV"
BASE COUNT          486 a          623 c          580 g          439 t
ORIGIN      
        1 tatctcaaca acagcattaa agcggtgaag gtgaatggga tcgagcccac gctgaaaaac
       61 gcgaaggcga agaagaaccg ctacccgatc gcccgcgagc tgtacatgta cgtgaacgaa
      121 aacaaataca ccccgcaggc gaaggcgttt gtgaaattcc tgctgggcaa aaagggacag
      181 gagctggtga agtccgcggg atatattccg ctctgatcat tttccacgca ttctactctc
      241 atcaccatgg aagggccgtg cgcccttcca ttttttcgtt cgcgcgccgc cgatcatctc
      301 gagaatggtc gcctcatagg gggcgagctc catgcggagc tcgaggaaat gctcatagcg
      361 cgaccgatgc gtcgagagga gcaccttcca atgcgcggat ttcttcaccg tgctggactt
      421 cgaggcgctc aaccagggct atgtggacgc gcgaatggag acgaaaacga gcgtctcgta
      481 gtcacggcac aatcacagca tcatgtatga ttcggccgcg ggagatcgcg gccgtttcgc
      541 gtcaggggat ccacgccaaa cgatcagcac gaagagggtc cagcgatgcc catcatgaga
      601 gcggattgaa gaccttcaat cccgcctttc tcgccgccgt tgcgagtttt gcatcgcatg
      661 cgacaaaaca atctatctcg tccctcacgg caagcgcggc gccgagttga atggagtcga
      721 gcgtccgcag ctgatgcgca tcaatgagcg cctttgcatt atctgcgaca acatcatcaa
      781 atggcacgca gacgaatgaa ggataatctg tcccgaactc gtttaacacc gtccgatagc
      841 cgtccgcatt cacgacacgg ttggcaagaa gtcgccggag cgcggagcac agttcgagtt
      901 ccgttatcgc cgatacgacg atctcatcag ccgtgcccat gatggcgtcc aagcgatccg
      961 acccttcctc gctgacatac cgcttaagca gggccgaggt gtcgatataa agcctcatcg
     1021 ctcatttcgc tcatgcatga tatagtcgag ggtagtggtt ccggtcttga gggtgatacg
     1081 tttcggctct cgcttccaag cggcgcccgt ttcgccaaag ggaacaattt tcgcaacggg
     1141 tttccccttg cgaatgatta taaaagaact cccacgttcc actttgtcaa agtactcctt
     1201 ggagcggttg cggaaatcgg taaacttgag atactgcata gtgcctccac tatcgcctac
     1261 tgtcataata atgacagacg gagaccatgt caagacaaat ggagacgaaa acgagcgtct
     1321 cgtagtcacg gcacaatcac agcatcatgt atgattcggc cgcgggagac cgcggccgtt
     1381 tcgcgtcagg ggatcagggg cgtaatacgg ctacactcga cgaatgacaa ccatggtcac
     1441 atcatccatg cattcgaatt gacggagcga ctggaggatg ccgtcgcgga tctcctcggt
     1501 cgggcgtgca cccagcgcgg cgagcgtttc gctgagccgg tgggccccga acatgtcacg
     1561 ctccggcgtg cgggtcccgg gacggctccc tcggctccat gcctcggtga tgccgtcggt
     1621 atacaccagc aacacatcgc cggaatcaag cgtgaacgaa ttgttgccga tcattccgct
     1681 gatatcctcc atgagcccga tccagacccc gtctgtttca atgacctcca ccgcggccga
     1741 cgaggcgcga tataccagaa tgtcctgatg caatccggcg aacacgaagc gcccgtcctt
     1801 gtgcgcggcg agcacggtta tggtcatgta cttgtcgtca ttgagcttcc tgacgttttc
     1861 cgttatggtc cgattaatca ccgtcaacaa cccggaggga tcgaggtccc ggttctggtc
     1921 aagcgccaca tgaatcgagg tctgcaccat catcataatg aggcccgccg gaaccccatg
     1981 ccccgaaaca tcgccgataa cgatccagtc gtttccgccg acactgatga cgtcgtagta
     2041 atccccgcca acctccgcgg ccggctccat atatgcggtg atctcatacc cgctgagctc
     2101 gggtcgttcc ggcaacaggg tggtctgg
//
LOCUS       DAORHF010000419         2106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_66245_length_2106_cov_3.529985, whole genome shotgun sequence.
ACCESSION   DAORHF010000419 DAORHF010000000
VERSION     DAORHF010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66245_length_2106_cov_3.529985"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PKJ16_19305"
     CDS             <1..998
                     /locus_tag="PKJ16_19305"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HNV49194.1"
                     /translation="PIRSLLKRIVFYTLTIEALVALSLMIFFSMRFPVADAAWHAVFH
                     SVSAFCNAGFSTFSDNLAGFHDHYAIQLLVCINIVLGGLGFIVLDELVAIRFRIFGRK
                     KFSTLTVHTRLSLVITGACIALGTVAFLLIEWKNVLQPLSLSQKFMASLFQSITARTA
                     GFNTVDIGALRPNTLFMLIILMFTGGCPGGIAGGVKTTTVGVIALQMYNKFKGNESIV
                     IWGRSIDRDTIDRCMTLVVISIITLSASTFLFQAFDDLPGNNSYLAAAFEVTSAFGTV
                     GLSTGITPQLSDMNKLLLCAVMYIGRLGPLTLMMAIASRAKKTNIHYPKTHIMTG"
     gene            1003..1677
                     /locus_tag="PKJ16_19310"
     CDS             1003..1677
                     /locus_tag="PKJ16_19310"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HNV49195.1"
                     /translation="MKKFIVIGLGNFGFHIAKTLVENDCEVLGIDSDHEIVERAKDHI
                     TYAIIGDAANREVLESLSIRDFDCAVVSVGRDMGMSILITLYLKEIGIPKIIVRAITE
                     DHGKILDMLGVSEISFPEKDMAIRLGFKLSLKNALDYLPIGAEYGIVEVNTPDSFVGK
                     NLKELAINTRFHCQIIGIKNNTGVAGASREQISIAPSPDEPLAKGSIMIVLGKEKDID
                     RIQSLG"
     gene            complement(1679..>2106)
                     /locus_tag="PKJ16_19315"
     CDS             complement(1679..>2106)
                     /locus_tag="PKJ16_19315"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV49196.1"
                     /translation="NLRILNKRMRHPGGCFGFRIEDRGKAFVFTSDCEFNIDEIDNIG
                     AYREFFNNAEVVVFDTQYTFEESINKIDWGHSSASIAIDIVSRFNVKRLILWHHEPDY
                     GDDKLESVLANAKAYLNMGGKRNSNLRVEIAYDGMEIDI"
BASE COUNT          480 a          612 c          525 g          489 t
ORIGIN      
        1 tccccatccg cagcctcctg aaacgaatcg tcttctacac cctgaccatt gaagcacttg
       61 tcgccctgtc gctcatgatt tttttcagca tgcggttccc ggtcgcggac gctgcatggc
      121 atgcggtttt tcactcggtg tcagcgttct gcaatgccgg cttctcgacc ttttccgata
      181 atctcgcagg ctttcacgat cactacgcca tacaattgct cgtctgcatc aatatcgtcc
      241 ttggcggcct tggcttcatc gttctcgatg aactcgtcgc tatccggttt cgcatattcg
      301 gtcgtaaaaa attcagcacg cttaccgttc atacgcgatt atcgcttgtg atcacgggcg
      361 cgtgcatcgc gctgggcacc gtcgcctttc ttctcatcga atggaaaaac gtgctacaac
      421 cattgtccct ttcgcagaaa ttcatggcga gcctcttcca gtcgattacc gcgcgaaccg
      481 ccggcttcaa cacggtcgat atcggcgccc tgcgccccaa tacgctgttc atgctgatca
      541 tcctcatgtt taccggcggc tgccccggcg ggatcgccgg cggagtgaaa acaacgaccg
      601 tcggagtcat cgcactccag atgtataata aattcaaggg gaacgaatca atcgtcattt
      661 gggggcgctc gatcgatcgt gacacgatcg atcgctgcat gacactcgtg gtgatctcaa
      721 tcatcacgct atcggcatcc acatttctct tccaggcgtt cgacgatctc cccggcaaca
      781 attcatatct cgccgccgcg ttcgaggtca cctcggcgtt cgggaccgtc ggcctttcaa
      841 ccgggataac gcctcaactt tccgacatga acaagctcct tctgtgcgcg gtcatgtaca
      901 tcggcaggct cggtccgctc acgctcatga tggcgattgc gtcacgtgcg aaaaaaacaa
      961 atattcacta tccgaaaacc catatcatga caggataaac acatgaaaaa gttcattgtg
     1021 atcggactcg gcaatttcgg cttccatatt gcaaaaacgc tggttgaaaa cgactgcgag
     1081 gtgctcggca tcgatagcga ccacgagatc gtggaacgcg cgaaagacca tatcacctac
     1141 gccatcatcg gcgacgcggc gaaccgcgag gtcctcgaaa gcctttccat cagggatttc
     1201 gactgcgccg tcgtgagcgt tggacgggac atggggatga gcatcctgat cacgctctac
     1261 ctgaaggaga tcggcatacc gaaaataatc gtgcgcgcca tcacggagga ccatgggaaa
     1321 atcctggaca tgctgggcgt aagcgagata tcctttcccg agaaggacat ggccatccgg
     1381 ctgggattca agctttccct gaaaaacgcg ctcgattatc tcccgattgg cgccgaatac
     1441 ggaatcgtcg aagtgaacac gcccgatagc ttcgtgggga aaaacctcaa ggaactcgcc
     1501 atcaatacac ggttccactg ccagatcatc ggcatcaaga acaatacggg cgtggcaggg
     1561 gcctcgcgcg agcagataag cattgcgcca tcacccgacg agcccctcgc aaagggatcg
     1621 atcatgatcg tccttggaaa ggaaaaggac attgaccgaa tccagtctct tggataggct
     1681 agatatcaat ctccatccca tcgtaggcga tctcgacgcg tagattgctg ttgcgttttc
     1741 cgcccatgtt caggtatgcc ttggcgttcg ccagcacgct ttcgagcttg tcgtctccgt
     1801 aatccggttc atgatgccag aggatcagcc gcttcacatt gaagcgcgaa acgatgtcga
     1861 tagcgatgga cgccgatgag tgcccccaat cgatcttgtt gatggactcc tcgaacgtgt
     1921 actgggtatc gaagacgacc acctcggcgt tattgaagaa ttcccgatat gcgccgatat
     1981 tgtcgatctc gtctatgttg aactcacagt cgcttgtaaa cacgaatgcc ttgccccggt
     2041 cctcgatgcg aaacccgaaa cagccgcccg gatgtcgcat tcgcttgttg aggatccgaa
     2101 gattgt
//
LOCUS       DAORHF010000420         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_66551_length_2100_cov_5.259658, whole genome shotgun sequence.
ACCESSION   DAORHF010000420 DAORHF010000000
VERSION     DAORHF010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66551_length_2100_cov_5.259658"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PKJ16_19320"
     CDS             complement(<1..884)
                     /locus_tag="PKJ16_19320"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HNV49197.1"
                     /translation="MATVSLARCPEYSRDAIRGRMMRSLEEIGFDPNRFRGARVAMKP
                     NLLLPAAPDKAIVTHPVFFEAALDIVIEHGGRPIVVESPALASVEKALRRSGYGPALD
                     RTGVGVADATGTAIVHNERGVAFKRFEIIREVCDAEFIINLPKMKTHGLTYFTGAVKN
                     YFGLVPGLEKAQWHLRAPTAAEFTSFLLDFYGAIRDGFSPRKTLLNIMDGICALEGEG
                     PGPGGRARFVGVMLASEDAVALDAVASAVVGLDREKIPTLAIAESRGLGVGALENITI
                     VGESVSSCAVSGFVPNRST"
     gene            complement(898..>2100)
                     /locus_tag="PKJ16_19325"
     CDS             complement(898..>2100)
                     /locus_tag="PKJ16_19325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49198.1"
                     /translation="SASSNTAASPFPLVADAYRSFRRGEYRDTVLLLEKIVEAGGRSP
                     YPIFLSALALLMTSQFARADALLKRIRALSPEYIPARQLEAFLFMKSSPAVEPAISAY
                     IDLVNRFPADRTLRRTLGVLRRVRDFTDFQQRARIADFVDIPRPRKADYARTTGSYGF
                     EIARRRGRFPAPGIGTIAIVGIILLIAGAGAFVAVRFDDIRNSIAPRIPGVAAPSHRI
                     DTVTLDASGYDLIEKLNRTRKPVFYYSSDEIASNFANAKMLIKKERYNEALSILNAIN
                     NSNANLMVKERVEFLIGFVNGVDNRAYESVPYREVEKSPYRYTGYAVLWKGKVANLAR
                     TQGRRIFNLLVDYRETNEFAGVAEVYSEDVSTPVENGDLIMVKGLFMYKIGSENRAYL
                     VAKEITKQ"
BASE COUNT          407 a          647 c          660 g          386 t
ORIGIN      
        1 gtggaccgat tgggaacgaa ccccgaaacc gcgcacgatg agacgctctc tccgacgatg
       61 gttatgttct cgagcgcgcc aacgcccaaa ccgcgtgatt cggcgatggc gagcgtgggt
      121 attttctcgc gatcgagccc gaccacggcg ctcgccacgg catcgagggc gaccgcatcc
      181 tcgctggcga gcataacgcc gacaaaccgc gcgcgcccgc cgggaccggg gccctccccc
      241 tccaatgcgc agatgccgtc catgatgttc agcaatgtct ttctcggtga aaaaccgtcg
      301 cggatggcac cgtagaaatc gagcaggaac gacgtgaact cagcggccgt cggcgcgcgg
      361 aggtgccatt gcgccttttc cagaccgggg acaagcccga aataattttt caccgcaccg
      421 gtgaaatagg tgagcccgtg cgtcttcatt tttgggaggt tgatgatgaa ctcggcgtcg
      481 cagacctccc ggatgatctc gaatcgcttg aaggcgacgc cgcgctcatt gtgcacgatc
      541 gcggtcccgg tcgcatccgc gacgccgacc ccggtccgat cgagcgcagg gccgtatccg
      601 gagcgacgaa gcgccttctc gacgcttgcc agcgccggtg actcgacgac gatcggtctg
      661 ccgccatgct cgatcacgat atcgagcgcc gcctcgaaaa ataccggatg cgtcacgatc
      721 gccttgtccg gagcggccgg gagaaggagg ttcggcttca tcgccacgcg ggcgccgcgg
      781 aatcgattcg ggtcgaaccc gatctcctcg agcgatcgca tcatgcgccc cctgatggcg
      841 tcacgcgaat actcgggaca ccgcgcgagt gaaaccgtcg ccatcacgcc gccgttctca
      901 ttgttttgta atctctttcg cgacaagata ggcccggttc tccgaaccga tcttgtacat
      961 gaagagtccc ttgaccatga tcagatcgcc gttttcgacg ggtgtcgaca catcctcgct
     1021 gtagacctcg gcgacaccgg cgaactcgtt ggtctcgcgg tagtcgacca ggaggttgaa
     1081 gattcgcctc ccctgcgtgc gagcgagatt ggccaccttc cccttccaga gcaccgcata
     1141 tcccgtatag cgatatggag acttctccac ctcgcggtag ggaacggact cataggcgcg
     1201 gttgtccacg ccgttcacaa atccgatgag gaactcgacg cgctccttga ccatgaggtt
     1261 tgcattgctg ttgttgatcg cgttgagaat cgacaacgcc tcgttgtagc gctccttctt
     1321 gatgagcatc ttcgcgttcg cgaagttcga cgcgatctcg tcgctggaat agtaaaagac
     1381 cggcttccgg gtgcggttga gcttttcgat gagatcgtac ccggaggcat cgagggtcac
     1441 ggtgtcgatc cgatgggagg gcgccgccac gcccggaatt cgtggcgcga tcgaattgcg
     1501 aatgtcgtcg aaccgaacgg ccacgaacgc tcccgccccc gcgatgagca ggattatgcc
     1561 gacgatggcg atggtcccga ttcccggcgc gggaaaccga ccgcgccggc gcgcgatctc
     1621 aaacccgtaa ctgccggtcg tgcgggcata atcggccttg cgcgggcgtg ggatatcgac
     1681 gaaatccgcg atgcgcgccc gctgctggaa gtcggtgaaa tcgcgtacgc gacgaaggac
     1741 gccgagcgtg cgtcgaagcg tgcggtcggc cggaaagcgg ttcaccagat cgatatacgc
     1801 cgatatcgcc ggttcgacgg ccggcgagga tttcatgaac agaaaggcct caagctggcg
     1861 ggcggggatg tattcgggtg aaagggcgcg gatccgcttg agcagcgcat cggcccgtgc
     1921 gaactggctg gtcatcagaa gcgccagcgc ggagagaaaa atcggatacg gcgatcggcc
     1981 ccccgcctcg acgatctttt cgagcaggag cacggtgtcg cggtactccc cccgacggaa
     2041 tgagcggtag gcgtccgcga cgagcggaaa cggcgacgcg gcggtgttcg atgatgccga
//
LOCUS       DAORHF010000421         2099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_66610_length_2099_cov_3.951076, whole genome shotgun sequence.
ACCESSION   DAORHF010000421 DAORHF010000000
VERSION     DAORHF010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66610_length_2099_cov_3.951076"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="PKJ16_19330"
     CDS             complement(<1..1186)
                     /locus_tag="PKJ16_19330"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HNV49199.1"
                     /translation="MRKIYSRIDEISGNVVTVQADGIKYGELAEITSAHGKSLAEVIL
                     LDGGKVSLQVFAGSRGISTGDEVRFLSQPMMVSFSDDLMGRIFNGRGTPRDNGSALTE
                     NLIEIGGPSVNPAKRIIPRNMIRTGIPMIDLFNSLVESQKLPIFSISGEPYNELLARV
                     ALQAEVDLIVLGGCGLKYDDYLFFKERLESGGAMSRSIFFVHTAADPVVECLMVPDIS
                     LAVAEKFALKGKRVLVLITDMTNFSDAMKEIAITMEQVPSNRGYPGDLYSQLASRYEK
                     AVDFDGAGSITILAVTTMPGDDVTHPVPDNTGYITEGQFYLKNGRIEPFGSLSRLKQL
                     VNGKTRDDHRALMDGMIKLYASYKETLEKKSMGFRMSDWDEKLLKYGTLFESELMDLS
                     VNI"
     gene            complement(1256..>2099)
                     /locus_tag="PKJ16_19335"
     CDS             complement(1256..>2099)
                     /locus_tag="PKJ16_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010256695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HNV49200.1"
                     /translation="EASVYTAVTMAEYYRQMGLHVLLLADSTSRWAQAMREMSGRLEE
                     IPGEEAFPAYLESVIATFYERAGIVRLKSGQIGSVTVGGTVSPAGGNFEEPVTQATLK
                     VVGAFHGLSRERSDARRYPAIHPLESWSKYQGIIDKKRTSFVRQALFASDEVGQMMKV
                     VGEEGTSLDDYMVYLKGEFFDSVFLQQNAFDKVDAATTGDRQKYVFDTIISIILATFD
                     MSDKESARSFFNQLRQRFLDWNGAPMDSDEFKKIEGEIMAMLEQHRKESAAEAAEKFK
                     QMFG"
BASE COUNT          376 a          632 c          655 g          436 t
ORIGIN      
        1 ggatattgac cgaaaggtcc atgagctcgc tctcgaagag cgtgccgtat ttgagcagct
       61 tctcgtccca gtcggacatc cggaagccca tcgatttctt ttccagcgtt tccttatagg
      121 aggcatagag cttgatcatg ccgtccatca gcgcgcgatg gtcgtcgcgc gtcttgccgt
      181 tcaccagctg cttcagtcgc gagagcgatc cgaagggctc gatccgtccg ttcttaagat
      241 agaactggcc ctcggtgatg tagccggtat tgtcgggcac ggggtgggtc acgtcgtcgc
      301 cgggcatggt tgtaaccgcc aggatggtga tggacccggc gccgtcgaag tcgaccgcct
      361 tctcgtagcg cgacgcgagc tggctgtaga ggtcgcccgg atacccccga ttggagggga
      421 cctgctccat ggtgatcgcg atctccttca tggcgtccga gaagttggtc atgtcggtga
      481 tcagcaccag cacgcgcttg cccttgaggg cgaacttttc cgcgacggcc aggctgatgt
      541 cgggcaccat gaggcattcc acgaccgggt cggcggccgt gtgcacgaag aaaatggagc
      601 ggctcatggc gccgccgctc tcaaggcgtt ccttaaagaa gaggtagtcg tcgtatttga
      661 gtccgcagcc tcccaggacg atgaggtcca cctcggcctg gagggcgacc cgcgccagca
      721 gctcattgta gggctcgccg gatatcgaga agatgggaag cttctgggat tcgaccagcg
      781 agttgaacag gtcgatcatg ggaatacccg tacggatcat gttccgcggg ataattcgct
      841 tggcgggatt gacggacggg ccgccgatct cgatgaggtt ttcggtgagc gccgatccat
      901 tgtcccgcgg cgttccgcga ccgttgaata tgcgccccat caggtcgtcg gaaaaggaga
      961 ccatcatcgg ctgcgaaagg aagcggacct cgtcgccggt ggatatcccg cggctccccg
     1021 cgaagacctg cagggaaacc tttccgccgt cgaggaggat cacctccgcc agggactttc
     1081 cgtgggccga ggtgatctcc gcgagctcgc cgtacttgat gccgtcggcc tgaacggtga
     1141 cgacattgcc cgaaatctca tcgatcctgc tatatatctt tctcatgtag gcctctcaaa
     1201 tgatgatatg cgctccacta tggtgcatcg cgaccggcgc cgaatccgcg ccgcgtcaac
     1261 cgaacatctg cttgaacttc tcggcggcct ccgcggccga ctctttccga tgctgctcga
     1321 gcatcgccat gatctcgccc tcgatcttct tgaattcgtc cgaatccatc ggtgcgccgt
     1381 tccagtcgag gaatcgctgc cgcagctgat tgaagaacga gcgggcgctt tccttgtcgg
     1441 acatgtcgaa ggtggcgagg atgatggaga tgattgtgtc gaaaacgtac ttctgtcgat
     1501 cgccggtggt tgcggcatcg accttgtcga aggcgttctg ctgcaggaag accgagtcga
     1561 agaactcgcc cttgagatag accatatagt cgtcgaggct cgtgccttct tcgccgacca
     1621 ccttcatcat ctggccgacc tcgtcgctgg cgaagagcgc ctgccgaacg aagctcgtcc
     1681 gctttttatc gatgatgccc tggtacttgc tccaactttc gaggggatgg atcgccgggt
     1741 agcgccgcgc gtcggaacgc tcgcgggaga ggccgtggaa ggcccccacg accttcaggg
     1801 tcgcctgggt gaccggctcc tcgaagttgc cgcccgcggg gctcaccgtt ccgcctaccg
     1861 tcacggaacc gatctggccg ctcttgaggc gtacgatccc ggcgcgctcg tagaaggtcg
     1921 cgatcacgga ctcgaggtag gccgggaagg cctcctcgcc ggggatttcc tcgagacgac
     1981 cggacatctc ccgcatggcc tgcgcccagc gggacgtcga gtccgcgagg agaaggacgt
     2041 gcagccccat ctggcggtag tattccgcca tggtgacggc ggtgtacacc gacgcctct
//
LOCUS       DAORHF010000422         2094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_66861_length_2094_cov_5.111820, whole genome shotgun sequence.
ACCESSION   DAORHF010000422 DAORHF010000000
VERSION     DAORHF010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66861_length_2094_cov_5.111820"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1477)
                     /locus_tag="PKJ16_19340"
     CDS             complement(<1..1477)
                     /locus_tag="PKJ16_19340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HNV49201.1"
                     /translation="MNKTSILVVSNDERFTGDITEVLGRLGYQVEVTGYSVTEIKAYL
                     RDTLDVILVHLPSTDYIDASVPIQTYCEINYLPVVFCLERIAENLLGLTLSRATYGYV
                     NVPVDENTLRATIELAMYRNVMDLVRKEAERRYRLLVETMNDGLIMVNAAGIILFLNQ
                     KTCDMTGYSSDEMLNRPFVDFFDEANRAIVEEQLAKRRQGVHDYYDIEWTCKNGRKIS
                     TIMSPQPLFDDTGHFRGSFAIITDITERKRAQDLMHIQHELAVSLSGIANLQDALRYV
                     VESAIRVEGMDSGGIYLVDPATGALDLACSIGFSDAFVSLSSHFDYNTPNANIVYRKM
                     PVYVDYSDLDVPDKKVLEGLQAIAILPIVYRNEVIGCLNVASHTVHGVPAAAREALET
                     IASSIASAIVRLRAEETLQRRNEELTAAKNELEAMNEELQAALEEMESVNEALIQSQD
                     ELIELNKMLNESTTLLQTVLDTIPARVFWKDRESRYLGCNMI"
     gene            1608..>2094
                     /locus_tag="PKJ16_19345"
     CDS             1608..>2094
                     /locus_tag="PKJ16_19345"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HNV49202.1"
                     /translation="MYIGIDIGGTGVKMVLVDAKGALHGFRSFRTGSSAQAIENGIVD
                     SAGALLASRRGGRHSLRGVGIGAPGQIDTGRGIIISSPNIPAWVRYPIATRVSALLGA
                     PAAIENDATVAVMGEQWLGHGRSYRNWIMLTLGTGIGGGAMIDNAIYLGRSGSATEFG
                     HT"
BASE COUNT          414 a          638 c          623 g          419 t
ORIGIN      
        1 agatcatgtt gcacccgagg tagcggctct cgcggtcctt ccagaacacc cgcgcgggaa
       61 tggtgtcgag caccgtctgg aggagcgtcg tgctttcatt gagcatcttg ttgagttcga
      121 tcagctcgtc ctgcgactgg atgagcgcct cgttgaccga ctccatctcc tcgagggcgg
      181 cctgcagctc ctcgttcatc gcctcgagct cgtttttcgc ggccgtgagc tcctcgttac
      241 gccgttggag cgtctcctcc gcgcgcaggc gaacgatcgc gctggcgatc gacgatgcaa
      301 tggtttccag cgcctcgcgg gccgcggccg gaacgccatg caccgtatgc gacgcgacat
      361 tgaggcaccc gatcacttca ttccggtata cgatggggag gatggcgatc gcctgcagcc
      421 cctccaacac cttcttgtcc ggaacatcca gatcggagta atcgacgtac accggcattt
      481 tccgatacac gatgttcgca ttcggcgtat tgtaatcgaa atgcgagctg agcgagacaa
      541 aggcatccga aaaaccgatc gagcaggcca gatcgagcgc gcccgtcgca gggtcgacga
      601 ggtaaatccc gcccgaatcc ataccctcca cccggatggc cgattccacg acatagcgga
      661 gcgcatcctg caggttcgca atgccgctga gcgatacggc aagttcgtgc tgaatatgca
      721 tcaggtcctg cgcccgtttt cgctcggtga tgtcggtgat gatggcgaag ctgccgcgga
      781 aatgtcccgt atcgtcgaag agcggctgcg gcgacatgat ggtggatatc ttcctgccgt
      841 ttttacaggt ccactcgata tcgtagtaat cgtgcacgcc ctgtcggcgt ttggcgagct
      901 gctcctcgac aatggcgcga ttggcctcat cgaagaaatc gacgaacggc cggttgagca
      961 tttcgtccga gctataaccg gtcatgtcgc aagttttctg gttaagaaag aggatgatcc
     1021 ctgcggcgtt caccatgatg agtccgtcat tcatggtctc gaccaggagt cggtaccgac
     1081 gctcggcctc cttgcggacc aggtccatca cattgcgata catcgccagc tcgatggtgg
     1141 cgcggagggt gttctcgtcg accggcacgt tcacgtagcc gtaggtcgcg cgcgacagcg
     1201 tgagccccag caggttttcc gctatacgct cgagacagaa caccacgggg agatagttga
     1261 tctcgcaata ggtctggatg ggaacgctcg catcaatgta gtccgtcgat ggcaggtgga
     1321 ccaggatcac gtcgagcgtg tcgcgaagat aggccttgat ctcggtcacg gaataaccgg
     1381 tcacctcgac ctgatacccc agccggccca ggacctcggt gatgtcgccg gtgaagcgtt
     1441 catcgttgct cacgacaaga atgctggtct tgttcatttc cgaacggtcc ccccggagaa
     1501 acggcccgac cgaatcatag aattattcct tgtttatttc aagaaataaa tttattccgg
     1561 tcacacagac cgccatgcgc ggcggcagcg ccgcagaaga ggggaccatg tacatcggaa
     1621 tcgacatcgg agggaccggc gtgaaaatgg tgctggtcga tgcgaaggga gcgcttcacg
     1681 gtttccgctc gttccgcacg ggatcatcgg cacaggcgat cgaaaacgga atcgtcgatt
     1741 cggccggcgc cctcctggcg agccgacgcg gcgggcgaca ttcattgcgc ggggtgggaa
     1801 tcggcgcccc cggccaaatc gacaccggcc gcggcatcat catctcgagc cccaacatcc
     1861 ccgcctgggt ccgctaccca atcgccacac gcgtgtccgc cctcctcggt gcgcccgcgg
     1921 ccatcgagaa cgacgccacc gtcgcagtca tgggcgagca atggcttggc cacggccgct
     1981 cctatcggaa ctggatcatg ctcaccctgg ggacgggcat cggcggcggc gcgatgatcg
     2041 ataacgccat ctatctgggc cgatcgggaa gcgcgaccga atttggccac acct
//
LOCUS       DAORHF010000423         2091 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_67024_length_2091_cov_4.933202, whole genome shotgun sequence.
ACCESSION   DAORHF010000423 DAORHF010000000
VERSION     DAORHF010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67024_length_2091_cov_4.933202"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..916
                     /locus_tag="PKJ16_19350"
     CDS             98..916
                     /locus_tag="PKJ16_19350"
                     /inference="COORDINATES: protein motif:HMM:NF015987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HNV49203.1"
                     /translation="MKIVTTFPRRSEYEKMAASLTHHGIPFEEIDAGIAYGRVGVAAI
                     ILDAEARSQLAAADGSSFLCAGWVDHHGPSSAIPSEQPPSFVEDLFGDAVVMVLAPCT
                     ADPKKIRVIAHTTKDMSPAFPYLNATMREGCYNPDGPLFTFMDCYRMITLYPQRIAAA
                     KADDIVDAWRMLEKIRRRVNEVYADRASLQPSYETRKKPPALEIYRRLPGTNCRECGE
                     KTCMAFALQLWNGNQKPESCAPLFSGEHQHLRSAYIEICAGLGFLDLDAREENV"
     gene            913..1062
                     /locus_tag="PKJ16_19355"
     CDS             913..1062
                     /locus_tag="PKJ16_19355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49204.1"
                     /translation="MNIKEVTMKFSESDVLRILAIALDDDEGDTLRLVQDTLAPYIKK
                     SLEPH"
     gene            1118..1576
                     /locus_tag="PKJ16_19360"
     CDS             1118..1576
                     /locus_tag="PKJ16_19360"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HNV49205.1"
                     /translation="MNKTKILFLCTGNSCRSQMAEGWANHLKGDAIEAYSAGVAPHSL
                     DPRAVRAMADAGVDISGHHSKHVSELVGIEFDYVVTVCGHAQETCPFFPGKTKVVHAG
                     FEDPPKLAEGAATDEEAMAHYRRVRDEIRVYIETLPEALINGSDGIIIQE"
     gene            1698..>2091
                     /locus_tag="PKJ16_19365"
     CDS             1698..>2091
                     /locus_tag="PKJ16_19365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV49206.1"
                     /translation="MSALNNRFSWTIRTERQRVSPDITQVYLELTSFCNLACTTCARH
                     SIAGFKPRHLSPSLFGKVIAALGELPRLERVVLLGFGEALCHPRAHELLSALRDAAPS
                     VVLVSNALLLDELARVHLVDGQRDDVYLS"
BASE COUNT          419 a          657 c          617 g          398 t
ORIGIN      
        1 gccttcgcca cggtggtggg gccgctggtg gaggtgccgg tactcatcgg gttggtgaac
       61 gttgcgttgt atttcaagaa gcggtttttc gcctaacatg aagatcgtca ccaccttccc
      121 gcgtcggagc gagtacgaaa aaatggcggc cagcctcacg catcacggca tcccgttcga
      181 ggagatcgat gccgggatcg catatggccg ggtgggcgtc gccgccatca tcctcgacgc
      241 ggaagcccgc tcacagctgg cggccgctga tggaagctca tttttatgcg cgggctgggt
      301 cgatcatcat ggcccatcga gcgccatacc gtccgaacaa cccccgtcat tcgtcgagga
      361 cctgttcggc gatgccgtcg tcatggtgct cgcgccctgc accgccgacc cgaagaagat
      421 ccgcgttatc gctcacacga cgaaggacat gtcgccggcg tttccgtatc tcaacgccac
      481 aatgcgcgag ggatgttaca atccagacgg tccgctgttc accttcatgg actgctaccg
      541 catgattacg ctctatccgc agcgcatcgc agccgccaag gcggacgata tcgttgatgc
      601 ctggcgtatg ctcgaaaaga ttcgccgccg ggtgaacgag gtgtacgcgg atcgcgcctc
      661 cctgcagccg tcgtacgaaa cacgaaagaa gccgccggcg ctcgagatct atcgacggtt
      721 gccgggtact aactgcaggg agtgcggcga aaagacctgc atggccttcg ccctgcagtt
      781 atggaacggg aaccagaaac ccgaatcgtg cgcgccactg ttctcgggcg aacaccagca
      841 ccttcgcagc gcctatatcg aaatatgcgc gggtctgggg ttcttggacc tggatgcacg
      901 ggaagaaaat gtatgaacat caaagaagtc actatgaaat tcagcgaatc agacgtgctg
      961 cgcatcctgg caattgcact cgacgatgac gagggcgaca cccttcgctt ggtccaggac
     1021 accctggctc cgtacatcaa aaagtctctc gagccgcact gagtcccggt tttcgaggcc
     1081 agttatcggc cccatcatcg aaacaggagc attgagcatg aataaaacca agatcctctt
     1141 cctgtgcacc ggcaactcgt gccgcagcca gatggccgaa ggctgggcga accacctcaa
     1201 gggcgacgcg atagaggcct attcggccgg cgtcgcgccg cattcgctcg acccgcgcgc
     1261 cgtgcgggcg atggccgatg cgggcgtgga catatcgggc caccattcga aacacgtgag
     1321 cgaacttgtg ggaattgagt tcgattacgt cgtgaccgta tgcggccacg cgcaggagac
     1381 ctgcccgttt tttccgggga aaacgaaggt ggtgcatgcc ggcttcgagg acccgcccaa
     1441 actcgccgag ggcgccgcga ccgacgagga ggcgatggcc cactaccgcc gcgtgcgcga
     1501 cgagatacgc gtctacatcg aaacgctgcc tgaagcgctt attaatggaa gcgatgggat
     1561 tattattcag gaataggatt ctccattgcc cccgcccgca gcccgcccgt acagacaggg
     1621 cattgccctg tctcaacgaa ggccctgtct caacgaaggc cctatctcaa cgccccgcca
     1681 cgctataatg aaccccgatg agcgccctca ataatcgctt ctcctggacc atccgcaccg
     1741 agcgccagcg cgtgtcgccc gacatcacgc aggtatacct ggaactcacg tcgttctgca
     1801 atctcgcctg caccacttgc gcgcggcact cgatcgcggg gtttaagccg cgccatcttt
     1861 cgccatcgct ctttggaaag gtgatcgcgg cgctcggaga actgccccga ctcgagcggg
     1921 tggtgctcct cggttttggc gaggcgctgt gccatccgcg cgcgcatgag ctgctcagcg
     1981 cgcttcgcga cgcggcgccc tcggtggtgc tcgtgagcaa tgcgcttctt ctggacgagc
     2041 tcgcccgggt acacctcgtc gatgggcagc gcgacgatgt gtacctctcg t
//
LOCUS       DAORHF010000424         2090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_67073_length_2090_cov_4.284029, whole genome shotgun sequence.
ACCESSION   DAORHF010000424 DAORHF010000000
VERSION     DAORHF010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67073_length_2090_cov_4.284029"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PKJ16_19370"
     CDS             complement(<1..392)
                     /locus_tag="PKJ16_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49207.1"
                     /translation="MEDHGHQRTHSEKIRIEAAEKIHRIPSPAKTPYQFLRCGKILRR
                     MQCRIDDIHAPHRAFPGCARRSIAPQPSTENLVNTRIRIYVSTSAAFREYRFAGKKST
                     RIPSVDHANGSANQNTLPAPYSESKPTTP"
     gene            450..>2090
                     /locus_tag="PKJ16_19375"
     CDS             450..>2090
                     /locus_tag="PKJ16_19375"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HNV49208.1"
                     /translation="MGLLSTLFLFFNAGYFIASSFYHPLAAYHRWITVGTILLCETHA
                     NMFLYYFHEERHPRLGKVLLYLQYLISIIVTIVFYVSTYGAEKVFLVNAHQWDFLADN
                     ISRMVGIVIQFFLAIFAIIMAWKMATIKTRERWVVLLLSLSYIVATLVPSIANTMSRS
                     GLLGRDAFQVLLNIFTVIGFTFMAILALNYTRDRTFVVVKIIGVSLVAILMLFQGFSF
                     FLYLEQEGSFDRLYAVFAQVAAADGEQPVASAYRARLDIDRKTVSVDHDESLGWQFGR
                     SIDPDVILNTALHVQIERLPDADFQRGLSRLLDASHRSFAGYRDSIRTYAGGLGDTAC
                     TRQGLFAYTANLQQELRRERNAIANIPSIDYRRSLLAYLQSSSRAGFVPFRNVLLGHL
                     ASSESEGSRLKEETLAYFSPFAPQGVRHYRTAAQHDFLTFHYVDMARRLAFEVGYPYL
                     AYRERMHPAAMRFVYILFVVVVIVALGFPLFFRGILIKPLARLVQGLERVERGDYHIQ
                     LPVQMKDEIGYVTENFNRMTGTVREAKMRLDNYTQTLED"
BASE COUNT          386 a          571 c          604 g          529 t
ORIGIN      
        1 ggcgttgtgg gtttcgattc cgaatacggc gcgggaagcg tgttctggtt tgcgctgccg
       61 ttggcgtgat cgaccgacgg gattctggtt gactttttac ccgcgaaccg gtattctctg
      121 aaggcggcag aagtggatac atagattcgt attcgggtat tgacgaggtt ctcggttgat
      181 ggctggggcg cgatggagcg tcgagcacaa ccgggaaacg ccctgtgggg cgcgtgaata
      241 tcgtcgatcc ggcattgcat gcggcgaagg atttttccgc accgcagaaa ctgatacggt
      301 gtcttggcgg gggaagggat acggtggatt ttttcggcag cttctatgcg aattttttca
      361 ctgtgggttc tttggtgccc atggtcttcc atggtttcct cgctataata tttctgtcca
      421 tccccaacaa gtccaaatcg acctttcata tgggcttgtt gtcgacattg tttttattct
      481 tcaacgcggg ctattttatc gccagcagtt tctatcatcc cctcgccgcc tatcatcgct
      541 ggatcacggt gggaaccatc ctcctgtgcg aaacgcatgc gaacatgttc ctctattatt
      601 tccacgagga gcggcacccc cgcctgggga aggtccttct gtatctgcaa tacctcatct
      661 cgatcatcgt cacgatcgtg ttctacgttt cgacctatgg cgccgagaag gtgttccttg
      721 tcaatgcgca tcagtgggat tttctagccg acaacatcag tcggatggtc ggcatcgtca
      781 tccaattctt tctggcgatt tttgcgataa tcatggcgtg gaagatggcg accattaaaa
      841 cgcgggagcg ttgggtggtc ctgctgttaa gcctgagcta tattgtggca accctggttc
      901 cgagcatcgc gaacacaatg agtcgttccg ggctcctcgg acgagatgcc tttcaggtgt
      961 tactgaacat cttcacggtc atcggtttca ccttcatggc catactggcg ctcaactaca
     1021 cgcgggaccg cacattcgtg gtggtgaaga tcatcggcgt gagtctggtg gccatcctta
     1081 tgctttttca aggattcagt tttttcctgt acttggagca ggaaggctca ttcgacaggc
     1141 tgtatgcggt ctttgcccag gtcgccgccg ctgacgggga gcagccggtt gcgtcggcgt
     1201 atcgcgcccg gctcgatatc gacaggaaaa ccgtatccgt tgatcacgat gaaagcctcg
     1261 ggtggcagtt cggtcggtcg atcgacccgg acgtgatcct caacacggcc ctccatgttc
     1321 agattgagcg gctccctgac gccgatttcc aacggggact ctcccgcctc ctcgatgcaa
     1381 gccaccgctc gtttgccggg tatcgggatt cgatccgtac gtatgccggg ggtcttggcg
     1441 acacggcctg tacccggcag gggctcttcg catatacggc gaatctgcag caggaactgc
     1501 gcagggaaag aaacgcgatc gcgaatattc cctcgatcga ttaccggcgg tcgctcttgg
     1561 cgtatctgca atcgtcttcg cgcgcgggat tcgttccgtt caggaacgtg cttctcgggc
     1621 acctggcctc gtcagagtcg gagggcagcc gtctcaagga ggagacgctc gcgtattttt
     1681 caccgttcgc tcctcagggg gtccggcact atcgcacggc cgcgcagcat gatttcctga
     1741 ccttccatta tgtggatatg gcccggcgac tggcgtttga agtcgggtac ccgtatctcg
     1801 cctaccgcga gcgcatgcat ccggccgcca tgaggttcgt atacatcctg ttcgtcgtcg
     1861 tggtcattgt cgcgcttggg tttccgctct tcttccgcgg catactcatc aaaccgctcg
     1921 cacggctggt ccagggtctc gagcgggttg aacggggcga ttatcacata caactccccg
     1981 tgcagatgaa ggacgagatc ggctatgtga cggagaactt caaccggatg accgggaccg
     2041 tcagggaagc gaagatgcgt ctcgacaact acacgcagac gctcgaggat
//
LOCUS       DAORHF010000425         2075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_67883_length_2075_cov_4.974257, whole genome shotgun sequence.
ACCESSION   DAORHF010000425 DAORHF010000000
VERSION     DAORHF010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67883_length_2075_cov_4.974257"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PKJ16_19380"
     CDS             complement(<1..469)
                     /locus_tag="PKJ16_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011696937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNV49209.1"
                     /translation="MSVKKVVLAYSGGVDTSVIVKWLIENYRCEVVCFAADVGQEEEL
                     DGLEAKAIKTGASTCYIEDIREEFVRDYLFQIIKANAIYENRYLLGTSIARPVIAKKQ
                     VEIALREGADAVCHGATGKGNDQVRFEMAFKALAPHLRIIAPWREWELNSRSVL"
     gene            complement(489..1448)
                     /gene="argF"
                     /locus_tag="PKJ16_19385"
     CDS             complement(489..1448)
                     /gene="argF"
                     /locus_tag="PKJ16_19385"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HNV49210.1"
                     /translation="MKIPSVAAKDLLTVTDLTRQEMLEIFKFSAKLKKETLAGKRHKF
                     LEGLTLAMIFEKSSTRTRVSFETGIFQLGGYALFLSKGDIQLARGETVADTARVLSGY
                     VDGIMVRAYEHGTLIDLARTATVPVINGLTDLYHPCQALTDYFTIYERDPAFPRIKLT
                     YVGDGNNMAHSLLLCGAILGADVAIASPKEYTCDRSIIERAFSIASSTGSRIDITTNI
                     DQAVAGAQYLYTDVWVSMGQEKGAAKRKKALSRYTITMPLLNKAAPGCRVMHCLPAHR
                     GEEITDEVLASNRSIVFEQAENRLHLQKGLMCAFMSKHKLFKK"
     gene            complement(1445..>2075)
                     /locus_tag="PKJ16_19390"
     CDS             complement(1445..>2075)
                     /locus_tag="PKJ16_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HNV49211.1"
                     /translation="IQGEGGIRPATREFASVLVETCKKHNMLLIIDEVQTGLGRTGKP
                     FAFQHYNLKPDAITLAKGLAAGLPLGALHARTDRAGLMEKGSHASTFGGNHLSCAAAR
                     VVLEEIAKPSFFKNMSAVSAYIFDRLATLKKELAIIADIRGKGLHVGIELTRPGAPLV
                     GAALARGLVINCTSERVLRLMPPLTVSRKTAREALDILEELLRVENGTQ"
BASE COUNT          380 a          607 c          647 g          441 t
ORIGIN      
        1 acagcaccga tcgtgagttg agctcccact cgcgccaggg tgcgatgatc cgcaggtgcg
       61 gcgcgagcgc cttaaaggcc atctcgaagc gcacctggtc gttgcccttg ccggtcgcgc
      121 catggcacac tgcgtcggcg ccctcgcgca gggcgatctc cacctgcttc ttcgcgatca
      181 ccgggcgggc gatggaggtg cccagaagat atcggttttc gtaaatagca ttcgccttta
      241 tgatctggaa caagtagtcg cggacgaact cctcccggat gtcctcgatg tagcacgtag
      301 atgcgccggt cttgatcgcc ttcgcctcga ggccgtcgag ctcttcctct tgtcccacgt
      361 ccgcggcaaa gcagacgacc tcgcagcgat agttctcaat gagccatttt acgatcacgg
      421 aggtgtccac tccgcccgaa tacgccagca cgaccttttt aacgctcatg gttttccccg
      481 gtgttcaatt atttcttgaa taacttatgc ttgctcatga atgcgcacat gagccccttt
      541 tgcagatgca ggcggttctc cgcctgctcg aatacgatcg atcggttcga ggcaaggacc
      601 tcgtccgtga tctcctcgcc gcgatgcgcc ggcaaacagt gcatgacgcg gcagcccggt
      661 gcggccttgt tcagcagggg catggtgatt gtatatcgcg agagcgcctt cttgcgttta
      721 gcggcgcctt tttcctggcc catgctcacc caaacgtcgg tgtaaagata ctgggcgccc
      781 gcaacggcct ggtcgatgtt cgtggtgatg tcgatgcggg agccggtcga tgaagcgatc
      841 gagaaggcgc gctcgatgat cgaccggtcg caggtgtact ccttggggct cgcgatggcc
      901 acatccgcgc cgaggatggc gccgcacagg agcagcgaat gcgccatgtt gttgccgtca
      961 ccgacatagg tgagcttgat acgcggaaat gccgggtcgc gctcgtagat ggtgaaataa
     1021 tcagtgagcg cctggcacgg atgatacaaa tccgtgaggc cgttgatgac cggaaccgtc
     1081 gcggtgcgcg cgagatcgat cagggtgccg tgctcgtagg cgcgcaccat gatgccgtcg
     1141 acgtaccccg agaggacgcg ggccgtgtcg gcgaccgtct cgccgcgggc gagctggatg
     1201 tcgcccttgc ttagaaagag cgcatagccg ccaagctgga agatgcccgt ctcgaacgac
     1261 acgcgcgtcc gcgtggagct tttttcgaaa atcatcgcca gcgtgagtcc ctcgaggaac
     1321 ttgtggcgct ttccggccag tgtctccttc ttgagtttcg ccgaaaactt gaagatttcg
     1381 agcatctcct ggcgggtaag atcggtaacg gtcaggaggt ctttagcggc gaccgatgga
     1441 attttcattg tgtgccgttc tccacgcgta aaagttcctc aaggatgtcg agcgcctcgc
     1501 gtgcggtctt ccgggaaacc gtgagcggcg gcatgaggcg aagcacccgc tcggaggtgc
     1561 aattgatcac caggccgcgc gcgagggccg cgccgacgag gggcgctccg ggtcgggtga
     1621 gctcgatacc cacatgaagg cccttgccgc gtatgtccgc gatgatcgca agttccttct
     1681 tgagcgtcgc gaggcgatca aaaatatacg cgcttactgc gctcatgttc ttgaagaacg
     1741 acggtttggc aatttcctcg aggaccaccc gggccgcagc gcaggagaga tgattcccgc
     1801 caaacgtcga ggcgtggctt cccttttcca taagtcctgc gcgatcggtc cgtgcgtgca
     1861 gcgcccccag cggaagtccg gcggcaaggc cctttgcgag ggtaatggcg tccggcttga
     1921 ggttgtagtg ctgaaaggca aagggcttac cggtgcgacc gagtccggtt tgcacctcgt
     1981 cgatgatgag gagcatgttg tgtttcttgc aggtttccac caggaccgac gcgaattcac
     2041 gcgttgcagg tcggatgccg ccctcgccct ggatg
//
LOCUS       DAORHF010000426         2066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_68376_length_2066_cov_4.256091, whole genome shotgun sequence.
ACCESSION   DAORHF010000426 DAORHF010000000
VERSION     DAORHF010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68376_length_2066_cov_4.256091"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            407..>2066
                     /locus_tag="PKJ16_19395"
     CDS             407..>2066
                     /locus_tag="PKJ16_19395"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HNV49212.1"
                     /translation="MALCLLIWTACAGCGPLSTKAKRPVAVKGVIDLRSWDFERDGAI
                     NLNGEWEFCWDRGLTSTDFVRIGSSEGCGYISVPGSWKDHTHFGAPLPGAGRATYRLR
                     ALFAPTKRTLTITIRRVFTAYRLWINGVLADSKGVMDESPRMKEDFSHVHDRRSSTFE
                     PRTGVNEIVLQVSNRDYASGGIDQPIRLSNTEYATQREFLLHTLDMIIVGILLFSAIY
                     NILFFFSRRRDIAPLYIGLTSLALALNTYNIQHPVLSTWLPHWANPLLVNHLTIITGT
                     TLFLMIAQSIFPGKFPTPILKLSLAVSACFSGVLVFTRAPIAERIIGVFYIIGLVWIL
                     FFAYGIFTTILKNRDEVVFFFIGLMSILLAGINNFLYSRTIIDTGNMLSYSMVIFCIM
                     STFVVSRRFARALETVEKMSRELTEKNIALEKADRLKDQFLANTSHELRTPLHGMIGL
                     SNSLLEGAAGGLSARTRDIIALIAASGTRLANMVNDLLDTTKIQDEGLPLDLRPVDLH
                     AVSTVVVRLALPLLGERPVEIVNRIGPDLPCAWADEDRVRQVLSN"
BASE COUNT          418 a          674 c          543 g          431 t
ORIGIN      
        1 cgaccggagc aggatcaggt cccattggcg tggcggtgaa tcccagcacc aatactgtgt
       61 acgtcataac ctataacaca cgcaccgtga tattcttcga tgcaaccacc ggagagtata
      121 agttttagcg ctctactcga taacggccct atcatcgcga tcaacacccc ccggtagaga
      181 caggtcggtg ccctatctct accgggccct ttggtccaga tcaacctctt atcaaccact
      241 attttcgttc ggtacagacg gggcatcgcc ccgtctctgg gcctggggca gggcaatgcc
      301 ctgccccaac gagtcccgtc cctatcgtac ttgacatgcc ctatttcccg catacaatgg
      361 ggcaatgccc acccacccat tatcgacacg aagactgttc accgcattgg ccctgtgcct
      421 cctcatctgg actgcatgcg cgggctgcgg cccgttgtcc acgaaggcga agcggcccgt
      481 cgcggtcaag ggcgtcatcg accttcggag ctgggacttc gagcgcgacg gcgcaatcaa
      541 cctgaacggc gaatgggagt tctgctggga ccgggggctt acctcaacgg actttgttcg
      601 gatagggagc agtgagggct gcggatacat ctccgtaccc ggttcctgga aagaccatac
      661 gcacttcggc gcgcccctgc ccggcgcagg ccgtgcgacc taccggctgc gcgcgctgtt
      721 tgccccgacc aagcggacac tcacgatcac catccgacgc gttttcacgg catacagact
      781 gtggatcaac ggtgtcctcg cggacagcaa gggcgtgatg gacgaatcgc cccgcatgaa
      841 agaggacttc tcgcatgttc atgaccggcg atcctcgaca ttcgaaccgc gcacgggcgt
      901 aaacgagatc gtcctgcagg tctccaatcg cgactatgca agcggcggta tcgaccagcc
      961 gatacggctt tccaacaccg agtacgccac acaacgggaa ttcctcctgc acacgctgga
     1021 catgatcatc gtcggcatcc tgctgttttc ggccatctac aatatcctgt tcttcttctc
     1081 gcgaaggcgg gacatcgccc cgctctatat cggcctcacg agcctggcat tggcactcaa
     1141 cacgtacaac attcagcacc cagtcctgtc aacctggctc ccccattggg ccaatccgct
     1201 gctggtgaac cacctcacga taatcacggg aacgacacta ttcctgatga tcgcacagtc
     1261 catctttcct ggaaaattcc cgacgcccat cctcaagctc tccctggccg tatctgcgtg
     1321 cttcagcggc gtactcgtat ttacacgcgc gccgatcgca gaaaggatca tcggcgtatt
     1381 ttacatcatt ggtctagtgt ggattctgtt tttcgcatac ggaatcttca cgacgattct
     1441 caagaaccgg gatgaggtcg tgtttttttt catcgggctc atgtcgatac tcctcgcggg
     1501 catcaacaac ttcctctact cccggaccat catcgacacc ggcaatatgt tatcgtattc
     1561 catggtgatt ttctgcatca tgagcacctt cgtcgtctcg cgccgtttcg cacgggcgct
     1621 tgaaacggtg gagaagatgt cacgggagct tacggagaag aacatcgcgc tcgagaaggc
     1681 ggaccgttta aaggaccagt tcctcgccaa cacctcacac gagcttcgaa cgccgttgca
     1741 cggcatgatt ggtctttcga actcgctcct tgagggcgcc gctggcggcc tctccgccag
     1801 gacccgcgac atcatcgcgc tcatcgcggc aagcggcacc cgactcgcga acatggtaaa
     1861 cgacttgctg gacacgacca agatccagga cgaggggctc cctcttgacc tgcgccccgt
     1921 agacctgcac gcagtgagca cggtggtggt acgcctcgcg ctcccgcttc tgggcgagcg
     1981 gcccgtggag atcgtcaacc gcatcgggcc cgacctcccc tgcgcctggg ccgacgagga
     2041 ccgcgtccgg caggtgctct cgaacc
//
LOCUS       DAORHF010000427         2061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_68643_length_2061_cov_4.847458, whole genome shotgun sequence.
ACCESSION   DAORHF010000427 DAORHF010000000
VERSION     DAORHF010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68643_length_2061_cov_4.847458"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="amrA"
                     /locus_tag="PKJ16_19400"
     CDS             <1..1104
                     /gene="amrA"
                     /locus_tag="PKJ16_19400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HNV49213.1"
                     /translation="HAVEIHLPFLQRIYGTRLERDAPILPILTGTVDERDATRIAAVL
                     ASVLRSKRAPVIIISTDFTHYGRRFGYLPFAASGMPDFSARMTALDRGAIDPILARTL
                     PAFARYVRNTGITVCGKHPIMIALALPIADAQTKLLAYDTSARITGDYATSVSYASIA
                     IAGALRAADIAAPAPDPDDADVPLTRADKQSLLSIARKNIQAHLSGSGPFRPPADGVT
                     PAMRSKRGLFVTLKVNDELRGCIGTVTGVKSIIDATLDNSFNAAFRDPRFPPLRRDEL
                     GLIRIEISVLTEPRPVGKPGDVVVGRDGLIMERGSQRGLLLPQVPVELGWDRNTFLSH
                     TCGKSGLPPDCWRDSRTKIKSFRAIVFGEPSKR"
     gene            1151..>2061
                     /gene="amrS"
                     /locus_tag="PKJ16_19405"
     CDS             1151..>2061
                     /gene="amrS"
                     /locus_tag="PKJ16_19405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HNV49214.1"
                     /translation="MLSRKELLRSACRALYAGACAPFLLTHDDACTIMAAQPDSPFIK
                     PARHWKKLAGGSVRCQLCPNECVISAGSRGACRVRMNLRGGLYTLVYGRLAAMHVDPI
                     EKKPLYHVLPGSLAYSVATAGCNMWCTFCQNWQISQAAPEAIDAAFVPPGQLSANARS
                     KRCPVIAYTYNEPSVQFEYLVDSARVARSRGIRPVIISNGYINDAPGRELLGVLDAVK
                     IDLKAFTNSFYAKVCGGDLHNVLKTLERIKRNGTWLEIVVLVIPTLNDAPGEIREMTR
                     WVRANLSPDVPMHFTRFHAMYRITNLPP"
BASE COUNT          366 a          732 c          590 g          373 t
ORIGIN      
        1 cacgccgtcg agatacacct cccgttcctc cagcgaatct acggcacgcg tctcgagcgc
       61 gatgcgccga tcctgcccat cctcacggga acagtcgatg agcgcgacgc gacgcgtatc
      121 gccgcggtgc tcgcgtccgt acttcgctcg aaacgcgctc ccgtgatcat catcagcacc
      181 gacttcacgc actatggccg caggttcggt tacctgccgt tcgcggcctc cggcatgccc
      241 gacttctccg cgcgcatgac cgcgctcgac cgcggtgcga tcgacccgat acttgcgcgc
      301 acccttcccg cgttcgcgcg atacgtccgc aacaccggca tcaccgtttg cggaaaacat
      361 ccaattatga tcgcgctcgc gctgccgatt gccgacgcgc aaacgaagct cctcgcctac
      421 gacacctccg cgcgcatcac cggcgactac gcaacgtcgg tgagctacgc ctcgatcgca
      481 atcgcaggcg ccctgcgcgc cgccgatatc gccgcgccgg ccccggatcc cgacgatgcc
      541 gatgtgccgc tcacgcgggc cgataagcaa tccctgcttt ccatcgcccg gaagaatatc
      601 caggcccatc tctccggatc gggcccgttc aggccgccgg ccgacggagt gaccccagcc
      661 atgcgatcca agcgcggatt gttcgtgacg ctgaaagtga acgatgagct tcgcggctgc
      721 atcgggacgg tcactggcgt gaaatcgatc atcgacgcca cgctcgacaa ctcgttcaat
      781 gccgcgtttc gggacccgcg cttcccgccc ctgcgacgcg atgagctggg actcatccgc
      841 atcgagatct cggtcctcac ggagccgcgc ccggttggca aaccgggcga tgtcgttgtc
      901 gggcgcgacg gactcatcat ggagcgcggt tcacagcgcg gcctgctgct cccgcaggtg
      961 ccggtggagc ttggttggga ccgaaacacc ttcctgtccc acacctgcgg aaaatccggc
     1021 cttccgcctg attgctggcg tgattcccgc acgaagatca aaagctttcg cgctattgtg
     1081 ttcggcgagc cgtcaaagcg ataggcggcg atacgcgtat gcgacgataa agaacgagcg
     1141 aacgatcatc atgctttcga gaaaagaact gctccgttcc gcatgccgcg cgctgtacgc
     1201 cggtgcgtgc gcgccatttc ttcttacgca cgatgatgcg tgcaccatca tggcggccca
     1261 gcccgattcg ccgttcatca agcccgcgcg ccattggaaa aagcttgcgg gcggttccgt
     1321 ccgctgccag ctctgcccca acgagtgcgt catcagcgcc ggcagccggg gagcctgtcg
     1381 cgtccgcatg aacctgcggg gcggtctcta tacactcgtc tacggacggc tcgcggccat
     1441 gcacgtcgat ccgatcgaaa aaaaaccgct gtaccacgtt ctccccggct ccttggccta
     1501 ctcggtcgcc acggccggct gcaatatgtg gtgcaccttc tgccagaact ggcagatatc
     1561 gcaggccgcc cccgaggcca tcgacgccgc cttcgttccg cccggccagc tttcggccaa
     1621 tgcgcgatcg aagcgttgcc cggtgatcgc ctacacctac aacgagccga gcgtgcagtt
     1681 cgagtacctc gtcgattccg cgcgcgtcgc gcggtcccgc ggcattcgcc cggtgatcat
     1741 ctcaaacggc tacatcaatg acgccccggg gcgcgagctg ctcggcgtcc tcgacgccgt
     1801 gaagatcgac ctcaaggcct tcaccaactc gttctatgcg aaggtctgcg gcggcgatct
     1861 ccacaacgtg ctcaagacgc tcgagcggat caagcgaaac ggcacctggc tggagatcgt
     1921 ggtgctcgtc atccccacgc tcaacgacgc gccgggcgag atacgtgaga tgacgcgctg
     1981 ggtgcgcgcc aatctctcgc cggacgttcc catgcacttc acgcgttttc acgcgatgta
     2041 tcggatcacg aacctgccgc c
//
LOCUS       DAORHF010000428         2024 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_70592_length_2024_cov_5.429660, whole genome shotgun sequence.
ACCESSION   DAORHF010000428 DAORHF010000000
VERSION     DAORHF010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70592_length_2024_cov_5.429660"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="PKJ16_19410"
     CDS             <1..870
                     /locus_tag="PKJ16_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005988686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HNV49215.1"
                     /translation="ERSYRSVYHRANHDVPMPAIERLSEIVELLRAILFPGYFGNSEV
                     KPETMGYYIGASVDTVYQLLAEQLKRGYCFSCAETETLDCADCERRARDIAVRFIERL
                     PEVRHLLSTDVAAAFEGDPAAKSSGEAIFSYPSIVALTNFRIAHELYRLDVPLIPRII
                     TEMAHSRTGIDLHPGAEIGERFFIDHGTGVVIGETSVIGRNVRLYQGVTLGAKSFPMD
                     DKGNPIKGIPRHPIVEDDVIIYSGATVLGRVTIGRGAVIGGNVWLTRDVPPGGRIMQG
                     APDENGFSGGGGI"
     gene            1035..>2024
                     /locus_tag="PKJ16_19415"
     CDS             1035..>2024
                     /locus_tag="PKJ16_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49216.1"
                     /translation="MEFLKSFYANYFTVGALIPACFHLFLAFLFLHIEKKSRSTFHIG
                     MGLVAMTVFNLAYTVAGSFYHPLAAFHRWVTVGVILIAETHFTMFAFYLCEEKRVTFG
                     KWFLVAQYAVALLVVAVFFAKTYGAGIVYHFAGNYYDFDAEGISRVVAICIILYILIF
                     FGVILWKVFTISGRDRWIVLAIGITYLLATVVPSVTNMLSRDGVLDRGTFQITWDLFN
                     ILGFFMMAILYINYTNDRTTFMAKIVGISLATLLLVLQGLSYVTFTDKEEAFDEIHRK
                     SAELVLRDDRHPHDLAYVTAFSPGADGFTRRYIASDAARDIDFHTRTVEFLNAA"
BASE COUNT          346 a          647 c          604 g          427 t
ORIGIN      
        1 gagcggtcgt accgctcggt gtaccatcgc gcgaaccacg acgttcccat gccggccatc
       61 gagcggctct cggagatcgt cgagctcttg cgcgccattc tctttcccgg atatttcggc
      121 aactccgagg tgaagcccga gacgatgggc tactacatcg gcgcgtcggt ggacacggtc
      181 taccagctcc tggccgagca gctcaagcgc ggctactgct tctcgtgcgc cgagaccgaa
      241 acgctcgact gcgccgattg cgagcggcgg gcgcgcgaca tcgccgtgcg cttcatcgag
      301 cggctgcccg aggtgcgcca tctgctctcc accgacgtgg ccgccgcctt cgagggggac
      361 ccggccgcca agagctccgg cgaggccatc ttcagctatc cgagcatcgt ggccctcacg
      421 aacttccgca tcgcgcacga gctctatcgg ctcgacgtgc cgctcatccc gcgcatcatc
      481 accgagatgg cgcactcgcg cacgggcatc gacctgcacc cgggggccga gatcggcgag
      541 cgcttcttca tcgaccacgg caccggcgtg gtgatcggcg agacctcggt gatcgggcgc
      601 aatgtccgtc tctatcaggg cgtgacgctg ggcgcgaaga gcttcccgat ggacgacaag
      661 ggaaatccca tcaaggggat cccgcgccac cccatcgtgg aggacgacgt gatcatctac
      721 tccggcgcga cggtgctcgg tcgcgtgacg atcgggcgcg gcgcggtgat cggcggcaac
      781 gtgtggctca cgcgcgacgt cccgccgggc gggcgaatca tgcagggcgc acccgacgag
      841 aacgggtttt ccggcggcgg cggcatctag gcgcgaatcc cttcggtacg atactgtaca
      901 tttcatttcc gcgtgttccg gcgtaaatat tttccccgcc cggtaaaata tgcttgaaat
      961 gacgccggtg gcgactatcg ttcaagccgt cctgtcgtat tataaagaga gatttccgtt
     1021 cgggtggcat tggcatggag tttctgaaga gtttttacgc gaattacttt accgttggcg
     1081 cgctcatacc cgcctgcttc catctgtttc tcgccttcct gttcctgcac atcgagaaga
     1141 agtcccgctc gacctttcac atcgggatgg ggctggtcgc gatgacggtc ttcaatctcg
     1201 cctatacggt cgccgggagc ttctatcatc cgctcgccgc gttccatcgc tgggtgacgg
     1261 tgggggtgat cctcatcgcc gagacgcact tcaccatgtt cgccttctat ctctgcgagg
     1321 agaagcgcgt aacgttcgga aaatggtttc tcgtcgcgca gtacgcggtg gccctgctgg
     1381 tcgtcgcggt tttcttcgcg aagacctatg gcgccggtat cgtgtatcat ttcgccggca
     1441 attactacga tttcgacgcc gaagggatca gccgggttgt cgcgatctgt attatcctgt
     1501 acatactgat cttcttcggc gtgatcctgt ggaaggtgtt cacgatctcg ggccgcgacc
     1561 gctggatcgt cctggccatc gggattacgt atttgctcgc gaccgtggtg cccagcgtca
     1621 ccaatatgct cagccgcgac ggcgtcctgg accgggggac cttccagatc acctgggacc
     1681 tgttcaatat tctcggcttt tttatgatgg cgattctcta catcaactac accaacgacc
     1741 ggaccacgtt catggccaag atcgtgggga taagcctggc gaccctgctt ctggtgctgc
     1801 agggcctcag ctatgtcacc ttcacggaca aggaggaggc cttcgacgag atccaccgga
     1861 aatcggcgga actggtgctg cgcgacgatc ggcatcccca cgacctcgcg tacgtgacgg
     1921 cgttctcgcc cggggccgac ggcttcacgc gccgatacat cgccagcgac gccgcgcgcg
     1981 acatcgattt ccacacccgc accgtcgagt ttctcaatgc cgcc
//
LOCUS       DAORHF010000429         1999 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_72038_length_1999_cov_5.230967, whole genome shotgun sequence.
ACCESSION   DAORHF010000429 DAORHF010000000
VERSION     DAORHF010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72038_length_1999_cov_5.230967"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PKJ16_19420"
     CDS             <1..508
                     /locus_tag="PKJ16_19420"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HNV49217.1"
                     /translation="NLAGDLKVSFDSVKSWLRTFDDFYVTFRIAPWSEKITRAITKEK
                     KLYLFDYALVGEEGARFENMVALELHRAVTGWTEQGNGDYALHYVRTKDGDEVDFLLA
                     RNGLPFLLVECKLSDNRPTPALLKIQSRLNIPAVQLVNQAGVYKLFSNDNLKVAVISA
                     DRWITSLP"
     gene            934..1593
                     /locus_tag="PKJ16_19425"
     CDS             934..1593
                     /locus_tag="PKJ16_19425"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HNV49218.1"
                     /translation="MPDASNRHDISFKLFLDALLAGDRPACSSIAWAYLDSGEPIISL
                     YEHIITRSLYEVGRLWEFNRISVATEHMATAIAEAIMNELYPRVVQTTHIGRAMVAAC
                     VESEEHQVGIKMVADIFEMRGWDAYFLGANIPTGELMSFIRTRRPSLVALSLAIYFNV
                     PLLAHMVAALRSAFPEISVLVGGQAFSHGPDVILRALGEVKHLPNLYSIEEYITHEWR
                     E"
     gene            1598..>1999
                     /locus_tag="PKJ16_19430"
     CDS             1598..>1999
                     /locus_tag="PKJ16_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49219.1"
                     /translation="MDRNMLLESAGRLKQATEASAAEYDAMRDSLVVRINERLLARED
                     IEFLVGPHNLAMMRDNHANHARFIASILKFHNPEALLDTIVWVFRAYRSRGFHVNYWA
                     AQLNAWMDVLKEMLSADAYADILPLYEWMQVN"
BASE COUNT          427 a          553 c          566 g          453 t
ORIGIN      
        1 caacctagcc ggcgacctca aggtttcctt tgattcggtc aagtcatggc tgcggacctt
       61 cgacgatttt tacgtcacct tcaggatcgc cccctggtcg gaaaaaatca cccgggcgat
      121 cacaaaggag aaaaaactat atctattcga ctatgccctt gtcggcgaag aaggcgcgcg
      181 cttcgaaaac atggtcgcgc tcgaactgca tcgcgccgtc accggctgga ccgaacaggg
      241 taacggcgac tacgccctgc actacgtacg cacgaaagac ggcgacgagg tcgacttcct
      301 gctggcgcga aacggcttgc cctttctcct ggtggaatgc aagctgtcgg ataatcgtcc
      361 cacgccggcg ctcttaaaga tccaatcgcg gctcaacatt cccgcggtgc agctggtcaa
      421 tcaggcaggc gtgtacaagc ttttttcaaa cgacaatctt aaggtcgcgg tgatttcggc
      481 tgatcgatgg atcacgtctt tgccatgaag cgtttcccaa cagcattata atggttctac
      541 agcccggcga tccgcttttc cggcgatgcg acggaggcgc cagtgctcgg cgaaattcaa
      601 ggccgatgtc acactctttt tggcccgaag aggactgtct gcatgagtga tccagttctc
      661 aatcggcagt atgtctcttg acgcattgcg gaaaggggcg gagtttcctt gatggtaaat
      721 ttacgcgtgg accaaggcca gcaggaacgt caccgattgg catgtttcgg cttgcacgat
      781 ggaaccgcgt attctttgat gcttgcagaa atttcttgtc cgtacgcgaa gttttctcct
      841 tgatcttgcg agacgcccgg tgcaagatat gcatcggtga attcgctcgg gcacgtgctt
      901 agtatgcatc atcaacgcga ggagccacgt ccgatgcctg atgcatccaa taggcatgat
      961 atatccttca agctttttct tgacgcgctt cttgcgggcg atcgccccgc gtgttcctct
     1021 atagcctggg catatctgga ttcaggagag ccaatcatat cgctgtacga acacatcatc
     1081 acgcggtccc tctacgaggt gggccgcctg tgggagttca accgcatcag cgtggcgaca
     1141 gagcacatgg ccacggcgat cgccgaggcg atcatgaacg aactctatcc ccgcgtggtg
     1201 cagaccacac acatcggcag ggccatggtt gccgcctgcg tggaaagcga ggagcaccag
     1261 gtcgggatca agatggtggc cgacatattc gagatgcgcg ggtgggacgc gtattttctc
     1321 ggggcaaaca ttcccacggg cgagctcatg tccttcatcc ggaccaggcg cccgtcgctt
     1381 gtcgcgcttt cgctcgccat ttacttcaat gtgccgttgc ttgcgcatat ggtggcggcg
     1441 ctgcggtccg cgttcccgga gatatcggtg ctcgtcggag gacaggcgtt ctcgcacggc
     1501 ccggatgtga ttcttcgtgc actcggcgaa gtgaagcatt tgccgaacct gtattcgatt
     1561 gaggaatata tcactcatga atggagagag tgacaccatg gacaggaaca tgcttcttga
     1621 gagcgcggga cggttgaaac aggcaacaga ggcgtcggcc gctgaatacg atgccatgcg
     1681 cgactcgctc gtggtgcgca taaacgaacg ccttctggcc cgcgaggaca tcgaatttct
     1741 ggtcggcccg cataatcttg cgatgatgcg cgacaatcac gccaatcatg cgcggtttat
     1801 cgcgtcaatt ctgaaattcc acaatccgga ggcgctcctc gacaccatag tgtgggtgtt
     1861 cagggcgtac cgcagtcgag ggttccatgt caactactgg gccgcgcagc tgaacgcctg
     1921 gatggatgta ttgaaggaaa tgctctccgc tgatgcatac gccgacatcc ttccgttata
     1981 cgaatggatg caggtcaac
//
LOCUS       DAORHF010000430         1987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_72756_length_1987_cov_3.337992, whole genome shotgun sequence.
ACCESSION   DAORHF010000430 DAORHF010000000
VERSION     DAORHF010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72756_length_1987_cov_3.337992"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PKJ16_19435"
     CDS             <1..299
                     /locus_tag="PKJ16_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49220.1"
                     /translation="ISTIVQSITRMDEAQRRALSTRLEIDPSHLATLTVRGYLALQFS
                     KGDRLGADVYREAVRHPILRTEKSGTRAVVTASNGMGIVFVKEGRYWLLDISDY"
     gene            complement(293..1159)
                     /locus_tag="PKJ16_19440"
     CDS             complement(293..1159)
                     /locus_tag="PKJ16_19440"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV49221.1"
                     /translation="MKASTAISTEAISLYNNALVFSNRGEYDHALTEYRKAIALCPGF
                     VEAYNNIGEIYSRMGDTSQAITTYQTALAIERNYKVLLNIGVEYYNKRDYRGALAFFT
                     ESLAKKPDFIEGNFYAGMAHFNMAAYEKAEQFFSRVVQFDRKHLKANYLLSYIYYEWK
                     DYGRTLECLDRIRDTADDRVFLNRYYGFCYYHLGRYDDAVAHLTDALEACPQYARFKG
                     YLKKLTYENKRKEMGDIDAKIREMEERMMRDAPTLSEYTRLSMLYIFQGEYKKAEDLL
                     LAAKGTRGQYPQ"
     gene            complement(1245..1745)
                     /locus_tag="PKJ16_19445"
     CDS             complement(1245..1745)
                     /locus_tag="PKJ16_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49222.1"
                     /translation="MSHSPEITFEMRNTRLSFDPERSILVCRQSGGSGKKLWAKKIFD
                     IGRIEEIIEDALCYYLAGASGETSGRLLALDKKSGNTAWFIAGKSVLQVLYQGHLFSI
                     FIDDTDAYYLVKIGLERGEVIWHHRVGSDLSEYHFAGDRITLDYSGGDRETLSIQNGS
                     VVSKTR"
     gene            complement(1755..>1987)
                     /locus_tag="PKJ16_19450"
     CDS             complement(1755..>1987)
                     /locus_tag="PKJ16_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49223.1"
                     /translation="WKTARMAALLGVKAREAERIIVEKGTEREEFLKNMVQFDSDDPY
                     NFHLIINNSTMSVDEAARLVIAAMRAKGQLPR"
BASE COUNT          393 a          578 c          548 g          468 t
ORIGIN      
        1 agatttctac gattgttcag tcaataacgc ggatggacga agcgcagcgc cgggccctct
       61 ctacgcgatt ggaaatcgat ccttcacatc ttgccacgct cacggttcgc ggctatctcg
      121 cgctgcaatt ttcgaagggc gatcgcctgg gcgccgatgt ctaccgcgag gccgtccggc
      181 atccgatcct tcgcaccgag aaatcgggca cccgtgcggt tgtcaccgcg tccaatggga
      241 tggggatcgt cttcgttaag gaaggccgat attggctgct tgatatcagt gactactgag
      301 gatactgtcc ccgcgttccc ttcgcggcaa gcagaaggtc ctcggctttt ttatactcgc
      361 cctggaagat atagagcatg ctcaggcgcg tgtactcgct gagcgtcggg gcgtcgcgca
      421 tcatccgctc ttccatctcg cgtatcttcg cgtcgatgtc ccccatctcc tttcgtttgt
      481 tttcataggt gagctttttc aggtaaccct tgaatctcgc gtattgcggg cacgcctcga
      541 gcgcgtcggt cagatgggcc accgcgtcat cgtaccggcc gagatgataa taacaaaacc
      601 cgtagtaccg gttcagaaac acccggtcgt cggcggtatc ccgaatgcgg tccaggcatt
      661 cgagcgttcg gccgtagtcc ttccactcgt aatagatata cgagagcaga tagttcgcct
      721 tgaggtgttt tcgatcgaac tgcacgaccc gcgaaaaaaa ctgttcggcc ttttcgtagg
      781 cggccatatt gaaatgggcc atgccggcgt agaaattccc ctcgataaaa tccggttttt
      841 tcgccagcga ctccgtgaaa aacgcgagcg cgccccgata atcgcgttta ttgtagtact
      901 ccacgccgat attcaggaga accttgtagt tacgctcgat cgccagcgcc gtctgatagg
      961 tcgtaatcgc ctgtgaggtg tcccccatgc gcgaataaat ctcgccgatg ttgttgtagg
     1021 cctcgacaaa acccggacac aatgcgatcg ccttccggta ctcggtgagc gcgtgatcgt
     1081 actctccccg attggagaaa accagcgcgt tgttgtacag ggaaatggct tccgtggaaa
     1141 tggcggtcga tgctttcatt ccgcaacctc gtttgatgtg attcgcgacc ggtgccggtc
     1201 ctcaggtaca ctatcggcac ggcgcgatga tgcggttgag cattttatcg ggttttggaa
     1261 acgaccgacc cgttctgtat ggacagcgtt tcgcgatcgc ccccggaata gtccagcgtg
     1321 atccggtccc cggcgaaatg gtattccgac agatcgcttc cgacgcgatg gtgccagatc
     1381 acctctccgc gctcgagccc gatcttgacg agataatagg catcggtgtc gtcaataaaa
     1441 atcgagaaga gatgtccctg atacagcacc tggagcaccg atttccctgc gatgaaccag
     1501 gcggtgttac cgctcttttt gtcgagcgcg agcagccgtc cgcttgtttc acccgacgcc
     1561 ccggcgagat aataacagag cgcgtcctcg atgatttctt cgatgcggcc gatgtcgaat
     1621 atcttcttcg cccagagctt tttcccggaa ccgcccgact gtcggcatac gagaatcgag
     1681 cgctcgggat cgaacgacag acgcgtgttt cgcatctcga aggtaatctc cggggagtgg
     1741 ctcatggcgc acgatcagcg cggaagctgt ccttttgcgc gcatcgcggc gatgaccagc
     1801 cgcgcggcct catcaacaga catggtgctg ttgttgatga tgaggtggaa attatagggg
     1861 tcgtcggaat cgaactgaac catgttcttc aggaattcct cgcgctcggt ccccttttcg
     1921 acgatgatgc gctccgcctc gcgcgccttg accccgagca gcgcggccat ccgcgccgtc
     1981 ttccact
//
LOCUS       DAORHF010000431         1970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_73747_length_1970_cov_5.163969, whole genome shotgun sequence.
ACCESSION   DAORHF010000431 DAORHF010000000
VERSION     DAORHF010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73747_length_1970_cov_5.163969"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PKJ16_19455"
     CDS             complement(<1..1197)
                     /locus_tag="PKJ16_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HNV49224.1"
                     /translation="MEESTHARRDILKLADALIFGAPPASFASAANASSSPAASSQDL
                     LARQFPKGFFWGTATSAYQIEGAWNEDGKGPSIWDTYAHTPGKIAKGDTGDIANDHYH
                     RYKEDVTLMKDIGANAYRFSIAWPRIFPQGTGTPNPKGVDFYKRLTDELREKGIEPFA
                     TLYHWDLPQALQDKYGGWRSADTAKAFGDYAGYVAEKLSDRIRYFFTINEFVSFTSMG
                     YQGRMVDVGSKKVRFEMAPGMDVGPSEIAQVRHHAVLGHGLAVQAIRARAKAGTMVGP
                     AEVIQVAVPMIEAPEHIKAAQTATRELNAEFLTVMLEGKYTDAYLAKLGKNAPKFNDA
                     DLKIIASPLDFVGINVYKPTFYVLASDERPGWRQIPFAKAHPRMLNSWLVFAPESIYW
                     APRFVQS"
BASE COUNT          415 a          559 c          559 g          437 t
ORIGIN      
        1 cgactggacg aatctgggtg cccagtagat cgactccggc gcaaatacaa gccagctgtt
       61 gagcatcctc ggatgcgcct tggcgaacgg gatctgccgc cagccgggcc gctcgtcgga
      121 ggccagcacg tagaacgtcg gcttgtagac attgataccg acgaagtcca aaggagaggc
      181 gatgatcttc aggtctgcgt cgttaaactt cggcgcattc ttcccgagct tcgcgagata
      241 ggcgtcggtg tacttgcctt cgagcatgac ggtgaggaac tccgcgttca gctcacgcgt
      301 ggctgtctgc gccgccttga tatgctctgg agcttcgatc atcggcacgg cgacctggat
      361 cacctccgcg ggaccgacca tggttccggc tttcgcccgg gcgcgaatgg cctgcaccgc
      421 gagtccatgg ccgaggaccg cgtgatgccg cacctgggcg atctcgctag ggccgacgtc
      481 catgcccggc gccatctcga agcggacctt cttgctgccg acgtccacca tgcgaccttg
      541 gtagcccatc gacgtgaagg acacgaattc gttgatggtg aagaagtagc gtatgcggtc
      601 ggagagcttt tctgcaacat agccggcgta gtcaccgaac gccttggcgg tatcggcaga
      661 gcgccagccg ccatacttgt cctgcagcgc ctgcggcagg tcccaatgat acagcgtggc
      721 aaagggctcg atcccctttt cgcgcaactc gtcggtcaat cgcttgtaga agtcgacgcc
      781 cttcgggttc ggcgtaccgg tgccctgcgg aaaaatgcgc ggccaggcga tggaaaagcg
      841 gtatgcattc gcgccgatgt ccttcatcag cgtgacgtct tccttgtagc ggtggtagtg
      901 gtcgttggcg atatcgccgg tgtcgccctt agcgatcttt cccggtgtgt gagcgtaagt
      961 gtcccagatc gacgggccct tgccatcctc gttccatgcg ccttcgatct ggtaggcgga
     1021 ggtagcggtc ccccagaaga aacccttggg gaattggcgc gccaacaaat cctgcgaaga
     1081 tgcagcggga gatgacgagg cattcgccgc cgatgcaaag cttgctggcg gcgccccgaa
     1141 aatcagcgca tcggcgagct tcagaatatc acgtctggcg tgagtggact cttccatagc
     1201 tacctcctgc gagatcgata ccgatcgaac tcatgccgtc cattatctcc ctgttgaatt
     1261 cagggatggg cctgtgcctc gacccaatgc tcttcgggca gtaatcgccg caatgaagga
     1321 gtctggcacg gcttcgaccc ggtcttggtt cattaccagg acatcacccg ctcgtggatc
     1381 aggaaagctc gttctactcc agattgcata aaaccggcgc atgacatgat aaatatggtt
     1441 ggaatttcaa atctcaataa gggctggttt tccgcatctt ccggaaactg aaaaaactcc
     1501 ggcttagcaa aaaaatacga tatgtacgct aaccacacaa ctgagatggg ccacgacaat
     1561 aggtgctgta cgcaaagtgt ttattggagc cacagcagca tagcgccgat gatccagagc
     1621 gcccgataga tcgtggcgga tttatcatag cgaacagaga gcgacctgaa gtgtttcatc
     1681 cgattgatta agcgctcgat gcgatttctc aaccgataga tgaccttgtc gaatttacgc
     1741 gttctggttt cattgatcct gtgcgggata gtccggactt gcaatatcgg gatcagtggc
     1801 gctttttcca ttcgacgtca cgctcggact tgaaacagat ggcgcctcct tcttcagggg
     1861 catagaaaaa gctcatcaga ttcgtctctc cgtcccggtc gaggtaatat acgttataca
     1921 ggacgacacc gggaacatcg cctttctccg ccttcacctt ctcgatcgag
//
LOCUS       DAORHF010000432         1942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_75496_length_1942_cov_5.939057, whole genome shotgun sequence.
ACCESSION   DAORHF010000432 DAORHF010000000
VERSION     DAORHF010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75496_length_1942_cov_5.939057"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="PKJ16_19460"
     CDS             complement(<1..1119)
                     /locus_tag="PKJ16_19460"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HNV49225.1"
                     /translation="MNTRVLMIYPEIPSTYWSMKHALQFVGNKSSMPPLGLMTIAAML
                     PARYDVRLLDMNVEPLTDEAIERADLVFISAMIVQKKSFEEAVARCRRFGKTIAAGGP
                     YPTSSHGAIAGVDHFVLGEGEITLPRFLADYEAGCALPCYECDEKPDISTTPVPRMDL
                     IDTTHYGAMALQYSRGCPFNCEFCDIIEMFGRKPRVKTPAQFVAELEAVYATGYRGSL
                     FVVDDNFIGNKRTVKELLPAVEAWQRERGFPFSLFTEASVNLAGDAELMALMVAAGFD
                     SVFLGIETPVEKSLEQAQKGQNMKGDLLTSVETIQRAGLEVMAGFIVGFDGDPDNVFD
                     LQIEFIRKSGIPMAMVGMLMALPKTQLHRRLEREGRLLA"
     gene            1383..1829
                     /locus_tag="PKJ16_19465"
     CDS             1383..1829
                     /locus_tag="PKJ16_19465"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HNV49226.1"
                     /translation="MTVVSVVTHEKVDPIVHSGLRAGMRAYRAAEHAHNPLGGPVAQA
                     FIVVDMPLSFIFDTALLPVTGPLALYDIYFDSANLALCAARGDIDGVRRASRQRFIDL
                     KAADRGGRTALHLAVLPRGRGDLPVPLGGDRSLPSRDSLARCGRFP"
BASE COUNT          352 a          591 c          606 g          393 t
ORIGIN      
        1 ggcgagcagc cgtccctcgc gctccaggcg tcgatggagc tgcgtcttgg gcagcgccat
       61 cagcatgccc accatcgcca tggggatccc cgatttcctg atgaactcaa tctgcaggtc
      121 gaagacattg tcggggtcgc cgtcgaagcc cacgatgaag cccgccatca cctcgagtcc
      181 cgcgcgctgt atcgtctcca cgctggtcaa aagatcgccc ttcatgttct gtcccttttg
      241 ggcctgctcc aggctcttct ccaccggcgt ctcgatccct aaaaagacgc tgtcgaatcc
      301 tgccgcgacc atgagcgcca tgagctcggc gtcgccggcg aggttcacgc tcgcttcggt
      361 gaacagcgag aagggaaagc cgcgctcacg ctgccaggcc tcgacggccg ggaggagctc
      421 cttcaccgtg cgcttgttgc cgatgaaatt gtcgtccacc acgaagagcg atccgcggta
      481 gccggtcgcg tacaccgcct ccagctcggc cacgaactgc gcgggcgtct tcacgcgcgg
      541 cttccggccg aacatctcga tgatgtcgca aaattcacag ttgaacgggc aaccgcggga
      601 atactgcagc gccatcgcgc cataatgcgt tgtgtcgatg aggtccatgc gcggaacggg
      661 cgttgtgctg atgtccggct tctcgtcgca ctcgtagcag gggagcgcgc acccagcctc
      721 gtagtccgcc aggaagcggg gcagggtgat ctcgccctcg cccaggacga agtggtccac
      781 gcccgctatc gcgccgtgcg acgaggttgg atagggtccg ccggccgcga tcgttttacc
      841 gaaccgacgg cagcgcgcga ccgcctcctc gaacgatttc ttctgcacga tcatcgccga
      901 tatgaacacg aggtccgcgc gctcgatcgc ttcgtcggta agcggctcca cgttcatgtc
      961 gaggaggcgc acgtcgtagc gcgccgggag catggccgca atcgtcatga ggcccagcgg
     1021 cggcatggag gatttattgc cgacgaactg cagcgcgtgc ttcatgctcc agtaggtgga
     1081 cgggatctcg ggatatatca tgagtactcg ggtgttcatg cggctctcct tgtcgtaact
     1141 ggccataggt atgtgtattg gcggcgtaat atgccacaaa ggacaggagc ggtcgcgtcc
     1201 ggttgcaaaa aaacgatcat gcgtccgata cactgaagcg gcgaccgaat ggcgtcgcgg
     1261 ataattcgtt gatgcgcgcc gatatagttg atataaaacg gatcggcggg gggccaggaa
     1321 caggtgtcga tccatgtgga gtgcgctgac ggcgctcgct tccctttttt atgtcgtcgt
     1381 gcatgaccgt tgtgtccgtc gtcacgcacg aaaaggtcga tccgatagtc cattcgggcc
     1441 tgcgcgcggg catgcgggcg tatcgggccg ccgagcacgc ccacaatccg ctgggcggcc
     1501 cggtcgcaca ggcgtttatc gtcgtcgata tgccgctgtc attcattttc gacacggcgc
     1561 tgttgccggt gacggggccg ttggcgttgt acgatattta tttcgacagc gcgaacctgg
     1621 ccctgtgcgc ggcccgcggc gatatcgacg gggtccggcg cgcttcgcgg cagcgattca
     1681 tcgacctgaa ggcggcggat cgcggcgggc gcaccgcctt gcatctcgcc gtgctgccgc
     1741 gggggcgcgg ggaccttccc gtgcccctcg ggggcgaccg ctcattaccc tcgagggatt
     1801 ctctcgccag atgcgggcga tttccatgaa acagatcgag ttgtacgcgg gttggcggcc
     1861 aaaaataatt tcgtttgaca gacacttcat tttactgata ccctgtgatt aatgaaagtt
     1921 attggcacaa aaaaaatcag gc
//
LOCUS       DAORHF010000433         1921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_76849_length_1921_cov_4.380493, whole genome shotgun sequence.
ACCESSION   DAORHF010000433 DAORHF010000000
VERSION     DAORHF010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76849_length_1921_cov_4.380493"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..>1921
                     /locus_tag="PKJ16_19470"
     CDS             77..>1921
                     /locus_tag="PKJ16_19470"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV49227.1"
                     /translation="MKRNRFVLAPDDSEGPRDERVGRNRISAAGAGGGRNMFAELRDS
                     RFFWPAVASLGIVAAIAATVLVVTTVVQRRGAPDSGQSAELRRDGRDTVYTDGEKKVL
                     PEEKTDNPGIARGRESYLRGYLADAISEFTAVVESGASERDKAIALTYIGIIHDDRGE
                     FDKAIEMYNRALRYDKRNPIIYRNLALAYRHKKEFEKAEEAIRAGLSISPDNVDNKIL
                     QGNILFEQGKFREARDMYDSVLKTDAGKPQALYNMGITLLKLGDLVSAMEYLKRAGNA
                     DRIGEVAHLAYAKLGVLYSQQRDLPEAEKYLRLAVAINPRDAVDRYNLGVVLLQQQKS
                     QEALEEFQKAEDLGKSDASVLENIGEAYSSLKQYERGIAAFERLYAINRRNVRVLSRI
                     AELYYEKGDLESAYEAYRRITQIEPATENARVAYLNMGNILDDAARHSEAVDAYLKAL
                     TINPKDDAALYNLGIAYKHMNKPELAIETWRKAADLNPSNPAPLLALADYYYETGHME
                     HAMDEFQKILRRFPGQQEGHFTLGTIYHKKGQNEYALEQFKRVIEINDKNELAQKAYI
                     NIGVLTSRSATDEKGLEQGLNYVQRALLLKPGDPEALVSMGTVYFKKEM"
BASE COUNT          411 a          603 c          626 g          281 t
ORIGIN      
        1 cagttcgtga acggcattct ggacgccatc aagaaaagcg agcgtgccga tgctcgcgaa
       61 ccgggaggtg gatccggtga aacgcaatag gttcgtgctc gcgcccgacg actccgaggg
      121 gccccgggac gagcgcgtcg ggcgaaatcg aattagcgcc gccggggccg ggggcgggcg
      181 gaacatgttc gccgagctgc gcgacagccg cttcttctgg cccgcggtcg catccctcgg
      241 catcgtcgcc gccatcgcgg cgaccgtcct cgtggtgacc accgtcgtcc agcgtcgggg
      301 cgcgcccgat tccgggcaat ccgccgagct gcgccgcgat gggcgcgata cggtgtatac
      361 ggacggcgaa aagaaggtcc tgcctgaaga aaagaccgac aatcccggca tcgcgcgcgg
      421 ccgcgagagc tatctgcgcg gctaccttgc cgatgcgatt tctgagttca ccgcggtcgt
      481 ggaatcgggc gcgagcgagc gggacaaggc gatcgcgctc acctatatcg gcatcatcca
      541 cgacgaccgc ggcgagttcg acaaggcgat cgagatgtac aaccgggcgc tgcgctacga
      601 caagcgaaat cccatcatct accgcaatct cgcgctggcc tatcgccaca agaaggaatt
      661 cgagaaggcc gaggaggcca tccgcgcggg gctctccatc agccccgaca atgtcgacaa
      721 caagatcctc cagggaaaca tccttttcga gcagggcaag ttcagggagg cgcgcgacat
      781 gtacgattcg gtcctgaaga ccgatgcggg caagccgcag gcgctctaca acatggggat
      841 caccctcctg aagctgggcg acctggtctc cgcgatggag tacctcaagc gcgcggggaa
      901 cgccgaccgt atcggcgagg tggcgcacct ggcgtatgcg aagctcggag tgctctacag
      961 ccagcagcgc gaccttcccg aggcggagaa atatttgcgg ctggccgtcg ccatcaaccc
     1021 ccgtgacgcc gtagaccgat acaacctggg cgtggtgctg ctccagcagc aaaagagcca
     1081 ggaggcgctc gaggagttcc agaaggccga ggacctcggc aagtccgacg cgtcggtcct
     1141 ggagaacatc ggcgaggcgt actcgtcgct caagcagtac gagcgcggga tcgcggcctt
     1201 cgagcggctc tacgcgatca accggcgtaa cgtgcgcgta ctctcgcgca tcgccgagct
     1261 ctattacgag aagggcgacc tggagtcggc ctatgaggcc tatcgacgca tcacgcagat
     1321 cgagccggca acggagaatg cgcgggtcgc ctatctcaac atgggaaaca tcctcgacga
     1381 tgccgcccgc cacagcgagg cggtcgacgc ctacctgaag gccctcacca tcaaccccaa
     1441 ggacgacgcg gcgctgtaca atctcgggat cgcctacaag cacatgaaca agccggagct
     1501 ggccatcgag acctggcgca aggcggccga cctgaacccg tcgaaccccg cgccgctgtt
     1561 ggcgctggcc gattactact acgagacggg gcacatggag cacgcgatgg acgagttcca
     1621 gaagatattg cgccgcttcc ccggacagca ggaggggcac ttcacgctgg ggaccatcta
     1681 tcacaagaag gggcagaacg aatacgccct cgagcagttc aagcgcgtga tcgagatcaa
     1741 cgacaagaac gagctcgcgc agaaggccta cattaacatc ggggtgctca cctcgcgctc
     1801 ggccacggac gagaagggac tggagcaggg gctcaactac gtgcagcggg cgctgctgct
     1861 caagcccggg gacccggagg cgctggtctc gatggggacg gtgtacttca agaaggagat
     1921 g
//
LOCUS       DAORHF010000434         1919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_76977_length_1919_cov_4.418455, whole genome shotgun sequence.
ACCESSION   DAORHF010000434 DAORHF010000000
VERSION     DAORHF010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76977_length_1919_cov_4.418455"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..1345
                     /locus_tag="PKJ16_19475"
     CDS             317..1345
                     /locus_tag="PKJ16_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49228.1"
                     /translation="MSIIKTPKTFVAIVIAGLLLVAANAFSWEFVDINFIPLNLENIS
                     RYNEDRIAYQQIQFSVPENGFSLVDNSFASLLIIRDRKMYLLYDGFDNAADVYQKKQI
                     QETENKLISNDALWVNKIDGKPDYIRITDRKIELMMKVDSAADGTTKAPAGAAAPKGE
                     KPASESEKNFVAKNFQTLYTNVRNEFIKRHVNIFRQLMIDRKESGLVVDRQPLPKRLF
                     LGHGTNEVTKYSTRVTAKTLDEKLYVAEDADGDGVCETFTVHLPDAFNWGNKSGPNII
                     FIYKNREKDIQEMIGALTNDAYYGTDEEAKTIKEEMRDSFPKEDDVQKVKNWIDNLVP
                     TIEQNTKK"
     gene            complement(1349..>1919)
                     /locus_tag="PKJ16_19480"
     CDS             complement(1349..>1919)
                     /locus_tag="PKJ16_19480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49229.1"
                     /translation="IAQRRIDALRGLGFALDNERVWQRTGGRAPAATSIIIEVFENDA
                     NRSDQRLAEYFGGPKESDRIINFYREYFGHGAAAYVPFDSIGIDEGIDCIHAAGGVAV
                     LAHPVFVRNRDWLDEIASMGLDGIEAISTYHTAEDISYYLGFAERRALAVTAGSDFHG
                     PTSKPLITIGGVTGNHYRYFEELAARVRR"
BASE COUNT          467 a          527 c          538 g          387 t
ORIGIN      
        1 gtcggtaaag ataaatccgt cggttccgca gtaataacat cgtgtgtgct ctttcatggt
       61 gcgcggggag cgtttctcgt catatccaga gtattgtcaa tgggctgtca ctcgaacagt
      121 ataacagtta cgacccgtcg gggcgaaata aaagaaaata tttgacaaaa atgccgatat
      181 tttatatagt tcaatggtat cgagtgtatt tcgtatgata cgtatgagct cccatgaggg
      241 gggatcatca atcccggtta ccgataaacg gagtttggcg gtaatgtgca ggtagaccag
      301 acagggaggt tccgcaatgt caatcattaa aaccccaaaa actttcgtcg cgatcgtcat
      361 cgccggtttg ctgctcgtcg ccgcgaatgc cttttcctgg gagtttgtcg atattaactt
      421 tataccgctt aatttagaga acatatcgcg ctacaacgag gaccgcatcg cctatcagca
      481 gatccagttt tcggtccccg agaacgggtt ttcgctcgtt gacaattcct tcgcaagcct
      541 tctgatcatt cgcgaccgta agatgtatct tctctatgac ggctttgaca atgccgcgga
      601 cgtgtatcag aagaagcaga ttcaggaaac ggaaaacaag ctcatcagca atgacgccct
      661 gtgggtcaat aagatcgacg gcaagccgga ttacatccgc atcaccgatc gcaagatcga
      721 gttgatgatg aaggtcgact ccgccgccga cggcaccacg aaggcccccg cgggcgcagc
      781 cgccccaaag ggcgaaaaac ctgcgtccga gagcgagaaa aattttgtgg cgaagaactt
      841 ccaaacgctc tacaccaatg tccgcaacga gtttatcaag cggcacgtga atatcttccg
      901 ccagctcatg atcgaccgca aggagtccgg gcttgtggtc gatcggcagc cgctccccaa
      961 acggctgttc ctcggtcacg gcaccaatga ggtcaccaag tactcgaccc gggtcacggc
     1021 gaaaacgctc gatgaaaagc tctacgtcgc cgaggacgcc gacggcgacg gcgtgtgcga
     1081 gacgttcacc gtgcatcttc ccgacgcctt caactggggg aacaagtccg gtccgaatat
     1141 tattttcatt tacaagaacc gcgagaagga cattcaagag atgatcgggg cgctgacgaa
     1201 cgacgcctat tacggaacgg acgaagaggc caagaccatc aaggaagaga tgcgggattc
     1261 atttcccaag gaagacgatg tccagaaggt caagaactgg atcgacaatc tcgtcccgac
     1321 catcgaacag aatacgaaga aatagccctc accggcgaac gcgcgcggcg agttcctcga
     1381 aatagcggta gtggttgccc gtcacccctc cgatggtgat gagcggcttc gaggtggggc
     1441 cgtggaagtc ggaaccggcg gtcacggcaa gcgcgcgtcg ttctgcaaag cccagataat
     1501 acgagatatc ctcggcggtg tgataggtcg agatcgcctc gatgccgtcg agtcccatcg
     1561 acgcgatctc gtccagccag tcccgattgc gcacgaagac ggggtgcgcg agcaccgcaa
     1621 ccccgccggc cgcgtgtatg caatcgatcc cctcgtcgat gccgattgaa tcgaacggaa
     1681 catacgccgc cgcgccatgc ccgaaatact cgcgatagaa gttgatgatg cggtcgcttt
     1741 cctttgggcc gccgaaatac tcggcgaggc gctgatcgga acggttcgcg tcgttttcga
     1801 agacctcgat gatgatggac gtggccgccg gggcccgccc gccggtgcgc tgccatacgc
     1861 gctcgttgtc gagcgcgaag cccagaccgc gcagggcgtc gatgcgacgc tgggcgata
//
LOCUS       DAORHF010000435         1909 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_77665_length_1909_cov_3.212513, whole genome shotgun sequence.
ACCESSION   DAORHF010000435 DAORHF010000000
VERSION     DAORHF010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77665_length_1909_cov_3.212513"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /gene="cdaA"
                     /locus_tag="PKJ16_19485"
     CDS             <1..575
                     /gene="cdaA"
                     /locus_tag="PKJ16_19485"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HNV49230.1"
                     /translation="VVITIVIVFQPELRRLIAQFGQRNWLSGAIFSESFPLDELVNAI
                     FSMAKEKVGSLIVIERNTGLKGYIESGVAINANISEELIRTIFHHNTLLHDGAIIIQG
                     ARIAAAACYLPLSESKQLKKSHGARHRAALGIAEESDALVIVTSEETGGITIMTNGKM
                     YPKIKFSDLKNMILSFMNPKTEKKETPVEQ"
     gene            593..1552
                     /locus_tag="PKJ16_19490"
     CDS             593..1552
                     /locus_tag="PKJ16_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49231.1"
                     /translation="MKDFAVRRKVVPKALSILLAIGLWLYVGQKKIGAVKLRIPVEYR
                     NLPKTLVIASNQNSFVAVELHGRKEDLNSVTIKNLRAYVNLESPNVGAKESYPIELVK
                     QQLPDNVEVALTQKDMSLVVERKETKTVRVKPVITGSIRSGHIMGIVRVTPDTVVATG
                     PESALRDLSAVPTRKISIENEKGRLVKIVAIDRSAIKKHLEFDKTSVNVVIPIVEASN
                     LVRVERPIELRNKNPHYLYEYENIVSKVMFRPRKPGIEVSGKDFRVIVDVASVPLDRM
                     FDEKQETRVTVELPVVVVPRPGLDIDVISVAPDSLSVTVIKKP"
     gene            1651..>1909
                     /locus_tag="PKJ16_19495"
     CDS             1651..>1909
                     /locus_tag="PKJ16_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49232.1"
                     /translation="MEAFIQTINPYSILPLSSSLLLLFIVISIICKYTKERLTLAVLF
                     FCVCISFWMFSSAVMLLFSDKDVSDLMIITYFLGWLLIPFAF"
BASE COUNT          451 a          529 c          511 g          418 t
ORIGIN      
        1 atgtcgtcat aaccatcgtc atcgtctttc agcccgagct gcgtcgcctc atcgcccagt
       61 tcggtcagcg aaactggctc tccggagcga tcttcagcga atcgttccca cttgacgagc
      121 tggtgaacgc cattttttcc atggcgaagg aaaaggtagg ttcgctcatc gtcatcgagc
      181 ggaacacggg gctcaagggc tatatcgagt ccggcgtcgc catcaacgcg aacatctccg
      241 aggagctgat ccggacgata ttccatcaca acacgctgct gcacgacggg gcgatcataa
      301 tccagggggc gcgcattgcc gccgccgcgt gctatctccc cctgagcgaa tcgaagcaat
      361 tgaagaaatc ccacggcgcc cggcatcgcg ccgctctcgg tatcgctgag gagtccgatg
      421 cgctggtcat cgtcacctcg gaggaaaccg gcggcatcac catcatgacc aacggaaaga
      481 tgtatcccaa gatcaagttc tccgacctga agaacatgat cctgtccttt atgaacccga
      541 aaactgaaaa gaaggagacc ccggtcgaac aatgaaggag ttttttaaaa cgatgaaaga
      601 tttcgccgtt cggagaaagg ttgttccgaa ggcattgtcc attctcctgg cgatcggatt
      661 atggctctac gtgggccaga agaagatcgg cgcggtgaaa ctgcgcatcc ccgtggagta
      721 tcgcaatcta ccaaaaacgc ttgttatcgc ctcgaaccag aacagctttg tcgccgttga
      781 attgcacggt cgcaaggagg acctgaacag cgtcaccata aaaaacctgc gcgcttatgt
      841 aaatctcgaa agccccaatg tgggcgccaa ggagagctat cctatcgaac tggtcaagca
      901 gcagctgccc gacaatgttg aggttgccct tacgcagaag gatatgtcgc tggttgtcga
      961 acggaaggag acaaaaaccg ttcgcgtgaa gccggtcatc accggttcga tccgtagcgg
     1021 ccacatcatg ggcatcgtca gggtaacgcc ggatacggtt gtcgcaaccg gtccggaatc
     1081 ggccctccgt gatctttccg ccgttcccac gaggaagatc tccatcgaaa acgaaaaggg
     1141 ccgcctggtg aaaatcgtcg cgatcgaccg cagcgccata aaaaagcatc tcgagttcga
     1201 taaaacgagc gtcaacgtcg tcatcccgat cgtcgaggcg agcaatctag tccgcgtaga
     1261 acgccctatc gaattgcgga acaaaaatcc ccactatctg tatgaatatg aaaatatcgt
     1321 gtcaaaggtg atgtttcggc ctcggaagcc gggaatcgag gtatcaggca aggacttccg
     1381 ggtcattgtc gacgtcgctt ccgtgccgct tgatcgcatg ttcgacgaaa aacaggaaac
     1441 gcgcgtgacg gtcgagcttc ccgtcgtcgt tgtgccgcgc cccggactcg atattgatgt
     1501 gatctcggtg gccccggaca gcctttcggt gacggtgatt aaaaaaccgt aggcggtccg
     1561 gtaaaacggg cgttcgtgcg attcgcgtgt ctgtaccctc atcggcggga cgtgcctgat
     1621 tgtcgccagg gagcaagcga aaaagttgcg atggaagcgt tcatacaaac aataaatcca
     1681 tattcgatac tgcccctttc cagctcgctc ctgctgttgt tcatcgtcat aagcattatc
     1741 tgcaaataca cgaaagagcg cctcacgctg gcggtcctgt tcttctgcgt gtgcatcagt
     1801 ttctggatgt tttcgtcggc ggtgatgctc ctgttctccg acaaggacgt ctcggacctc
     1861 atgatcatca cgtacttttt gggatggctc ctcatcccgt tcgcgtttt
//
LOCUS       DAORHF010000436         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_78295_length_1899_cov_4.280911, whole genome shotgun sequence.
ACCESSION   DAORHF010000436 DAORHF010000000
VERSION     DAORHF010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78295_length_1899_cov_4.280911"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PKJ16_19500"
     CDS             complement(<1..449)
                     /locus_tag="PKJ16_19500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HNV49233.1"
                     /translation="MGIKDNFDRIRSEADGICRAYGRDPVSLRIIAVSKTHPAETVQE
                     AIESGILLFGENKVQEAQKKIPLLTGSPVFHLVGHLQSNKARQAVALFDLIHSIDRLT
                     TAAAVNDEAARIGKVQKVLVQVNTSYESSKNGVSPDEAATLVHQMSDL"
     gene            915..1850
                     /locus_tag="PKJ16_19505"
     CDS             915..1850
                     /locus_tag="PKJ16_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49234.1"
                     /translation="MISPVCDTPYGADKKWRGDAGKTVILMEVVRHALQELGVDFDAY
                     APRCDLPIALDGRIATPGVRALNAVSAGAAALGWSHGCSLARRFASAYPSLVTDFAQM
                     CPSGYVNWGNMALAGPARLLVRFLTECLNISMRATFSHPVKATLSGLNVMKGNTIDTI
                     NAVSSLIDGNKHPMKLLRNMRDLKECAIYLDSYDAPMAHVKNAVIIFGEHDSLFNLMK
                     ISGVKRRSEIGQKEIDRFWATYYPDIDVDSSNVRVMVADGDHIAPYVHHTLYPELVLK
                     GLGLYHDENATPVRPAVVTRRPRVKRSRQMTRKTA"
BASE COUNT          436 a          515 c          513 g          435 t
ORIGIN      
        1 aggtccgaca tttggtgaac aagcgtagcg gcttcgtcgg gagatacgcc gtttttggat
       61 gattcgtacg acgtgttcac ctgtacgagg actttctgga cttttccgat ccgtgccgcc
      121 tcgtcattca ccgccgcagc ggttgtaagc cggtcgattg aatgaatgag atcgaacaag
      181 gccaccgcct ggcgcgcctt gttcgactgt aaatgaccca ccaggtggaa cactggagat
      241 cccgtgagta aggggatctt tttttgggcc tcctggacct tgttttcgcc aaatagcaga
      301 atcccgctct cgatggcctc ctgaacggtc tcggctggat gtgttttgct gacggcaatg
      361 atgcgaagcg acacagggtc acggccgtat gctcgacaaa tcccatccgc ttcggatcga
      421 atccgatcaa agttgtcttt tatccccata gctccattga gtagagtcat taaaatttag
      481 caatacaaaa aatattttat gagcaattca gatgcaaaat atccattcag taccagttaa
      541 caggaattga taagtaaaaa ttcgaaaagt caattgaccc atattgaccg tccggccaac
      601 ttttattata attaaattga tttattaaac aacagcaata acgttatcta agatagaaac
      661 cagaggttag aatgttcaag agagctcttc caaagaaacc agctacgcga aaggtaaaag
      721 tttcattcaa tggcgagact ctgagcgcct actatgcggt taccgacagc cgtacgtccg
      781 acgaaaagtg gctcaatcaa aaaaatggac ggttgtcggg tccggtaatc gttttctttc
      841 aggggcatgc ccagcggccg gctgatattt tcccgtttac cgcggagttg tcctttaggt
      901 gcacgtccgg ggttgtgata tcacccgtat gcgatacgcc ctacggtgcg gataaaaaat
      961 ggcgtggcga tgccggtaag accgtcattc tcatggaggt cgtccggcat gccctgcagg
     1021 agctgggagt ggatttcgat gcgtatgcgc cccggtgtga tctgccgatc gcgcttgacg
     1081 gacgcatcgc gacacccggc gtcagggcgc taaatgccgt ttccgccgga gcagccgcgc
     1141 tgggttggtc gcacggttgc agtctcgcac gccgattcgc cagcgcctac ccgtcgctgg
     1201 taacggattt tgcccagatg tgcccctcgg ggtacgtgaa ctgggggaat atggccctgg
     1261 ccgggccggc gcgcctgctg gtgcgctttc tcaccgagtg cctcaatatc tcaatgcgcg
     1321 cgaccttctc gcaccccgtg aaggcgaccc tctcgggact taacgtcatg aaggggaaca
     1381 cgatcgatac catcaatgcc gtttcgtcgc tgatcgacgg caacaagcac ccgatgaagc
     1441 tcttgcggaa catgcgggac ctcaaggaat gcgcgatcta cctcgacagc tacgatgcgc
     1501 ccatggcgca cgtgaaaaac gcggtgatca tcttcggcga acacgattcc ctgttcaatc
     1561 tcatgaaaat tagcggggtg aagcggcgct ccgaaatcgg ccagaaggaa atagaccggt
     1621 tctgggcgac ctattatccc gacatcgatg tcgattcttc caatgttcgc gtaatggtgg
     1681 ccgatggcga tcatatcgct ccctacgtgc accatacgct ctatcccgag ctggtcctca
     1741 agggccttgg cctgtatcat gacgagaacg ccacgcccgt tcgcccggct gtcgtaaccc
     1801 gccggccccg cgtgaagcgg tcccgtcaga tgacgcgaaa gacggcctag ctcaactgcg
     1861 catggatgat ccgcgaaatc ccgcttgcag ggcgggatg
//
LOCUS       DAORHF010000437         1888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_79012_length_1888_cov_5.269504, whole genome shotgun sequence.
ACCESSION   DAORHF010000437 DAORHF010000000
VERSION     DAORHF010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79012_length_1888_cov_5.269504"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PKJ16_19510"
     CDS             <1..546
                     /locus_tag="PKJ16_19510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HNV49235.1"
                     /translation="PSAHRERVALSVEEFVTMGRFPHRRPWEIAREDDHAAVAAAIES
                     VGIAGLARRPLTELSGGELQLARIALALAQDCGVILLDEPTSFLDIAHAVRIMDALHG
                     MHRRGKTIITVLHDINTAADYCTRIVGIGGGRIAFDGPPAAVLTYENLEALYGTTCIV
                     YDNAVTKKPHVFTVPEYARRG"
     gene            complement(560..>1888)
                     /locus_tag="PKJ16_19515"
     CDS             complement(560..>1888)
                     /locus_tag="PKJ16_19515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49236.1"
                     /translation="YRAGVVERVVRILKEGGSAEPMEEKFLTADGRIIDVEVTGVPIM
                     FKDRPSVMVLIHNVTEKKAMERELVQARRLDSIGRLAGGIAHDLNNMLTPVLGYSSML
                     VHNVTPGTEEHECLMQIEKAAERAKNLTMQLLTFSRKRTIDLKAVSLGRVVRDFEPIL
                     RRTIREDIRIELGIGAEGLLVMADVSQIEQILINLAVNAEEAMPDGGVFTISLEPVNR
                     PSKPAVSNGVAGLGVALTVGDTGRGMDAETLEHAFEPFYTTRSNGTGLGLATVYGIVR
                     QHDAAIDIRSAPGAGTAVVITFPAVEGREEEMRAQRPSMDSPAAGITILVVEDEEAVL
                     RLAERILGSVGYRVLTASNGPDALAAAERHAGPIDLLLTDVIMPGMDGKRLYESLGRL
                     HAGLPVVFMSGHHRDVLAGRDIDSAGTGFIQKPFTAPELIRQVRDVLGKR"
BASE COUNT          317 a          634 c          612 g          325 t
ORIGIN      
        1 ccatcagcac atcgagaacg tgttgccctt tcggtggagg aattcgtgac gatggggcgc
       61 ttcccccacc ggcgcccgtg ggagatcgcg cgcgaggacg atcacgccgc cgtcgccgcg
      121 gcgatcgagt cggtcggaat cgccgggctc gcccggcggc cgctcacgga gctctcgggc
      181 ggggagctgc agcttgcgcg catcgcgctc gccctggccc aggactgcgg ggtgatcctc
      241 ctggacgagc cgacctcgtt tctggacatc gcccacgccg tgcgcatcat ggacgcgctc
      301 cacggcatgc accgccgggg gaagacgatt atcacggtgc tccacgacat caacacggcg
      361 gccgattact gcacgcgcat cgtcgggatc ggcggcgggc gcatcgcctt cgacgggccg
      421 cccgcggcgg tgctcaccta cgagaacctc gaggcgttat acggaacaac ctgcatcgta
      481 tacgacaacg ccgtgacgaa aaaaccgcac gtgttcaccg tgcccgaata cgcacgacgg
      541 ggataggggc gttcgcggat cagcgttttc cgaggacgtc gcggacctgc cggatgagtt
      601 ccggcgcggt gaagggcttc tggataaacc cggtccccgc ggagtcgatg tccctgcccg
      661 ccaggacgtc ccggtgatgt cccgacatga agacgaccgg gagcccggca tgcagccgac
      721 cgagcgattc atagagccgt ttcccgtcca tccccggcat gatcacatcg gtgaggagca
      781 ggtcgatggg gcccgcgtgc cgctcggccg cagccaacgc atccgggcca ttggacgcgg
      841 tgagcacgcg atagccgacc gatcccagga tccgctccgc caatcgcagg accgcttctt
      901 cgtcctcgac caccaggatc gtgattccgg ccgccggaga gtccatcgag gggcgctgcg
      961 cccgcatctc ctcctcgcgt ccttcaacgg cgggaaaggt aatgacaacc gccgtccccg
     1021 cgccgggtgc gctccggata tcgatcgcgg cgtcgtgctg ccggacgatg ccatacaccg
     1081 ttgcgagacc aagaccggtg ccgttcgatc gggtggtata gaagggctcg aaggcgtgct
     1141 ccaacgtttc ggcatccatc ccgcgaccgg tgtcgccgac ggtgagcgcc acgccgagtc
     1201 ccgcgacgcc attcgacacc gcgggcttcg aagggcgatt gaccggttcg agggatatgg
     1261 tgaacactcc gccatcgggc atcgcctcct cggcgtttac ggcgagattg atgaggatct
     1321 gttcgatctg cgacacgtcc gccatcacca gaagcccttc cgcgccgatc ccgagctcga
     1381 tgcggatgtc ctcccggatc gtgcgccgca ggatgggctc gaaatcgcgc acaacgcgcc
     1441 ccagcgacac cgccttgagg tcgatcgtcc gcttccgact gaaggtgagc agctgcatcg
     1501 tgaggttctt cgcgcgctcg gcggccttct cgatctgcat gaggcactcg tgctcctcgg
     1561 tgccgggcgt cacgttatga acgagcatgc tcgagtagcc gaggacgggc gtgagcatgt
     1621 tgttcagatc gtgggcgatg cccccggcga ggcggccgat ggaatcgagc cggcgcgctt
     1681 ggacgagctc gcgctccatg gctttcttct cggtcacgtt gtggatgagg accatgaccg
     1741 agggacggtc tttgaacatg atcgggaccc cggtcacctc gacgtcgatg atccgcccgt
     1801 cggcggtgag gaatttttcc tccatcggtt cggcgctgcc gccctccttg agaatgcgca
     1861 ccacccgttc gaccacgccg gcccgata
//
LOCUS       DAORHF010000438         1881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_79508_length_1881_cov_3.601314, whole genome shotgun sequence.
ACCESSION   DAORHF010000438 DAORHF010000000
VERSION     DAORHF010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79508_length_1881_cov_3.601314"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..253)
                     /locus_tag="PKJ16_19520"
     CDS             complement(14..253)
                     /locus_tag="PKJ16_19520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49237.1"
                     /translation="MMRIAACAIAIAVCLTAGIVHGQKPDEQRIMVAYTYYVVSGNTD
                     EMLSGIRAAARERGGYVKFFSKDRIVIRLPHKETA"
     gene            complement(250..1455)
                     /locus_tag="PKJ16_19525"
     CDS             complement(250..1455)
                     /locus_tag="PKJ16_19525"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNV49238.1"
                     /translation="MTVRIAHIRSILLVCLVGIFAACGTAQYRSEATGGSRPGSPAAY
                     SKMKSERYAGKRALDSLADEMRSPKEEEADESPKPAKPVERMVVYFAKYSLQVESVRK
                     SMADVQAIVGAAGGFIESAVTSDSYRAARIVIRVPVAEFEKTLAALERVGEVSAKEVS
                     AADVTMEYRDISLRMEMARTVRDRLYQLLKAAKKADDRVKILREIERLTTQIDSMNAR
                     MAYLKNRATLSTIELRLAAKVREVTRSYLPSPFPWIAGLDPNKRTIFDSGGSVSYDAM
                     SGFFHMKDEYYDRSGAYLFMTPDRSTGMRIGTVDNYPPANQEFWREALRLELSNRMYT
                     IRKEDAIVAGAARFMSYEIALAKGVYVLSVAVTDENIVIVETVFPDDASYKKYAGQIV
                     KFIQTIRVK"
     gene            1663..>1881
                     /locus_tag="PKJ16_19530"
     CDS             1663..>1881
                     /locus_tag="PKJ16_19530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49239.1"
                     /translation="MFEAIMLVCFGCSWPLSIIKSVRAKRVEGKSIFFLWLIFIGYMA
                     GIANKIVTRPDWVTLFYAVNGAMVMADII"
BASE COUNT          347 a          573 c          559 g          402 t
ORIGIN      
        1 gatagcgcgc gagctatgcc gtttccttgt gggggagccg tatcacaata cggtccttcg
       61 aaaagaattt cacgtagccg ccgcgttcgc gcgccgccgc gcgtatgccg gaaagcattt
      121 cgtcggtatt ccccgacacc acataatagg tataggcgac catgatccgt tgctcatcgg
      181 gcttctggcc atgtacgatt ccggcggtaa ggcacacggc gatggcgatg gcgcaggcgg
      241 ctatccgcat catttcaccc gtatcgtctg aatgaatttc acgatctggc cagcgtattt
      301 tttatagctc gcgtcgtcgg gaaacacggt ctcgacgatg acgatatttt cgtccgtgac
      361 cgcaaccgag agaacgtaca cgcccttcgc gagcgcgatc tcgtagctca tgaagcgtgc
      421 agcgcccgcg acgatggcgt cctctttgcg gatcgtatac atgcggttgg acagctcgag
      481 ccgaagggcc tcccgccaga attcctggtt cgccggggga tagttgtcca ccgtgccgat
      541 ccgcatcccc gtgctgcggt cgggcgtcat gaagaggtac gcgccgctgc gatcgtaata
      601 ctcgtccttc atgtggaaaa aaccgctcat cgcatcgtag gacacggacc cgccggaatc
      661 gaagatggtc cgcttgttgg gatcgaggcc ggcgatccag gggaacggcg atggcaggta
      721 actgcgcgtg acctcgcgca ccttcgcggc gagccgaagc tcgatggtgg aaagcgtggc
      781 gcggtttttc agatacgcca tgcgcgcgtt catggaatcg atctgcgtgg tgagacgctc
      841 gatctcccga aggatcttta cgcggtcgtc cgctttcttc gccgccttca ggagctgata
      901 gagccgatcg cgcacggtgc gcgccatctc catgcgaagc gagatatcgc gatactccat
      961 ggtcacgtcg gcggccgaga cctccttcgc gcttacctcg cccacgcgct cgagcgccgc
     1021 gagcgtcttt tcgaactcgg cgaccgggac gcgtatcacg atccgggccg cccggtagct
     1081 gtccgacgtc acggcggact cgatgaaacc gcccgcagcg ccgacgatcg cctggacatc
     1141 ggccatcgac ttgcgtacgc tttcgacctg aagcgagtat ttcgcgaagt acacgaccat
     1201 ccgctccacc ggcttcgcgg gcttcggaga ctcatcggcc tcttcctctt tcggggagcg
     1261 catttcatcg gcaagactgt cgagcgcgcg ttttccggca tagcgctcgc tcttcatttt
     1321 cgagtaggcg gcaggcgagc ccggccgtga gccgccggtg gcctcggagc gatactgcgc
     1381 ggttccgcag gctgcgaaga tgccgacaag gcagacgagc aggatcgagc ggatatgagc
     1441 gatacgaact gtcatggcgt attattgcag ctgcaatgca ttctgtcaag ctgaataatc
     1501 agctcccgga ctcccggaat cctcgttaat accgtcgtgg cgcttcgccc tcggttcctt
     1561 cctgaaaacg gataatggac ttgacgcggg ttgccgcgtt cattcgttaa atacgcggcg
     1621 gacgcggccg cgcctccgcc gacatcatac ggagggcctt ccatgttcga agcgatcatg
     1681 ctcgtttgtt tcggctgctc gtggccgttg tccatcatca agtccgttcg cgcaaagcgg
     1741 gtcgagggaa aatccatctt cttcctgtgg ctcatcttca tcgggtatat ggcgggaatc
     1801 gccaacaaga tcgtcacgcg tccggattgg gtcacgctct tctacgccgt caacggcgcc
     1861 atggtgatgg ccgacatcat c
//
LOCUS       DAORHF010000439         1870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_80246_length_1870_cov_4.736088, whole genome shotgun sequence.
ACCESSION   DAORHF010000439 DAORHF010000000
VERSION     DAORHF010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80246_length_1870_cov_4.736088"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PKJ16_19535"
     CDS             <1..525
                     /locus_tag="PKJ16_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1921 domain-containing protein"
                     /protein_id="HNV49240.1"
                     /translation="GCAFDIILKREIQTANPANLKYGLNARPSPDERELVVTFVDNHD
                     MGASPYSPANGWGQQCWPCPPYFKSMAYAYILTMPGTPCVYWPDCFDWGLKEEIRRLI
                     AARKKAGIVARSGWTDLTNRHTGFAGIVQNENGEETLAVSIGSDYAGPGAGWSVAAEK
                     RGEWTVWIPKKLNC"
     gene            575..>1870
                     /gene="der"
                     /locus_tag="PKJ16_19540"
     CDS             575..>1870
                     /gene="der"
                     /locus_tag="PKJ16_19540"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HNV49241.1"
                     /translation="MAGKLPIVAIIGRQNVGKSTLFNALIRSKKAIVDPYPGLTRDII
                     SCVVTAGESSFVLADTPGLDIASSAELSQSIVQKTKEFLHECAAVVLLLENPLPAPFD
                     LDLIELLRKLERPVIVAVNKMDTIEDLANMPHFYEVGFNDIVPISAEKRRNLDLLLDK
                     VTAVIPRRAPAEETPDLRVAIVGRPNSGKSTLLNAFMGYERALVSDIPGTTRDAVDER
                     FRFQGKLIAISDTAGIRRKSRITERVEYFSLDRALGSIRASDIVVHLVDAEQGLTETD
                     KKISDEIMKAMKPVIIAINKWDVIEKDHTTFNAMKDRIHFKFYKAADFPIISVSARDR
                     LRIHKLMETVIDLYERSSLHIETGRLNRTLETILKKHRPPKVGSTLKVYYATQTGTCP
                     PQLVMFVNRPELFTKDIHRFFEKRLQEELAIPGVPVIIRV"
BASE COUNT          396 a          633 c          534 g          307 t
ORIGIN      
        1 ggctgcgcct tcgacatcat tcttaagcgc gagatacaaa cggccaatcc cgcaaacctg
       61 aaatacggac tcaacgcccg ccccagcccg gatgagcgcg agctggtcgt caccttcgtg
      121 gacaaccacg acatgggcgc ctcgccctac agtcccgcga acggctgggg acagcagtgc
      181 tggccgtgcc cgccctactt caagtcgatg gcctacgcct atatcctcac catgccgggc
      241 accccctgcg tctactggcc cgactgcttc gactggggac tcaaggaaga gatacgccgg
      301 ttgatcgccg cgcggaagaa ggccgggatc gtcgcgcgct ccggatggac cgatctcacc
      361 aaccggcata ccggcttcgc ggggatcgta cagaacgaga acggcgaaga gacgcttgcc
      421 gtatccatcg gttcggacta cgccggtccg ggagccggat ggtccgtcgc cgcggagaag
      481 cggggcgaat ggaccgtgtg gattccgaaa aaattgaatt gctaaaaatg ggccactccc
      541 gacccttctc ccggtacgag gagaaggaac acgcatggcc ggaaaactcc ccatcgtcgc
      601 aatcatcgga aggcaaaacg tgggcaaatc cacgctcttc aacgcgctca tccgctcgaa
      661 gaaggcgatc gtggacccct accccggcct cacgcgcgac atcatctctt gcgtggtgac
      721 ggcgggcgaa tcctccttcg tgcttgccga caccccgggt ctggacatcg cctcctccgc
      781 ggagctctcg cagagcatcg tccaaaagac gaaggaattt ttacacgagt gcgcggccgt
      841 cgtcctcctg ctcgagaacc cgctgcccgc gcccttcgac ctcgacctca tcgagctcct
      901 gcgcaagctc gagcgtccgg tgatcgtggc cgtgaacaag atggacacca tcgaggacct
      961 ggcgaacatg ccgcacttct acgaggtcgg gttcaacgac atcgtgccca tctcggcgga
     1021 aaagcggcgc aacctcgacc tcctgctgga caaggtcacc gcggtgatcc cccggcgcgc
     1081 ccccgcggag gaaacgccgg acctccgggt cgcgatcgtg gggcgcccca actcggggaa
     1141 atcgaccctg ctcaacgcat tcatgggcta cgagcgcgcg ctggtctccg acatccccgg
     1201 cacaacgcgc gatgcggtgg acgagcgttt ccgctttcag ggaaagctca tcgcgatttc
     1261 cgacaccgcc ggcatccgcc gcaagagccg catcaccgag cgcgtggagt acttctccct
     1321 cgaccgcgcg ctcggatcca tccgcgccag cgacatcgtg gtccacctcg tggacgccga
     1381 gcaggggctc accgaaaccg acaagaagat ttccgacgag atcatgaagg ccatgaagcc
     1441 ggtcatcatc gccatcaaca aatgggacgt cattgaaaaa gaccacacga ccttcaacgc
     1501 catgaaggac cgcatccatt tcaagttcta caaggccgcc gattttccca tcatctcggt
     1561 ctcggcgagg gaccggctgc gcatccacaa gctcatggaa acggtgatcg acctctacga
     1621 gcgttcgtcg ctgcacatcg agaccgggcg gctcaaccgg acgctcgaga ccatcctcaa
     1681 aaagcaccgg ccgccgaagg tgggatcgac gctcaaggtc tactacgcca cgcagacggg
     1741 cacctgcccg ccgcagctgg tcatgttcgt gaaccggccg gagctcttca cgaaggacat
     1801 ccatcggttt ttcgagaagc gattacaaga ggagttggcg atccccggcg tcccggtgat
     1861 catccgcgtc
//
LOCUS       DAORHF010000440         1849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_81697_length_1849_cov_4.262542, whole genome shotgun sequence.
ACCESSION   DAORHF010000440 DAORHF010000000
VERSION     DAORHF010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81697_length_1849_cov_4.262542"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PKJ16_19545"
     CDS             <1..487
                     /locus_tag="PKJ16_19545"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HNV49242.1"
                     /translation="PELASRDHVRKLLPLVREFGYPVTLFVYADTIYSRADTNLTWSR
                     LREMQQAGIDVQSHTISHRDLVRLSASDNPDARKRLFDELVMSRRLIELYLKKPVRLL
                     AFPYGHYDDRVIDVSRRAGYTRVFSTDYGPNIITRNNFCLRRRHIKSDYSLAVIQSLV
                     Q"
     gene            693..1433
                     /locus_tag="PKJ16_19550"
     CDS             693..1433
                     /locus_tag="PKJ16_19550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HNV49243.1"
                     /translation="MKHTTKYLCIALILLLATAVSAHSIQVDSNAIQQYRGSLGKWIQ
                     VDSPGTLHAYAKKFSVDPKDVYTINGTNGYGPLVNNYVFFPYSKEYLKELESKGITRQ
                     TVEDQADKFFWPIRTFGHISSVLGNRWGRFHPGVDIPSTRETPIHAAQSGRVIYARYC
                     GGYGLTVAIEHKNNLMTRYAHNSVNLVKEGDFVQKGQIIALVGSTGNSTGNHLHFEVR
                     CNDIPLDPLDFLPRIENIQKPHSIKSWK"
     gene            1548..>1849
                     /locus_tag="PKJ16_19555"
     CDS             1548..>1849
                     /locus_tag="PKJ16_19555"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HNV49244.1"
                     /translation="MTDFDTPIDLHIHSSFSEDADYPVDAIFAMAHESGVSVISITDH
                     DSVESIPVALSLTGAHPVRYVPGVELTTMFPRDGSQQHVLGYFVDPDNRRLQETLTG"
BASE COUNT          433 a          580 c          462 g          374 t
ORIGIN      
        1 ccccgagctg gccagccggg accacgtccg caagctcctg cccctggtgc gggaattcgg
       61 atatccggtc accctgttcg tgtacgccga taccatctat tcccgcgccg acaccaatct
      121 cacctggtcg cgactccggg agatgcagca ggccggcatt gacgtgcaga gccatacgat
      181 aagccaccga gatctggttc ggctttcggc cagcgataac cccgacgccc gaaagcgcct
      241 cttcgacgag ctcgtcatgt cccgccgatt gatcgagctg tacctgaaga aaccggtccg
      301 gctcctggcc tttccctacg gccattacga cgatcgcgtc attgacgtga gtcgccgcgc
      361 cgggtataca cgcgtttttt cgaccgacta cggcccaaat attatcaccc gtaataattt
      421 ttgtctgcgc cgcagacata tcaaaagtga ttacagcctt gccgtaatac aaagccttgt
      481 gcaataaaaa agcacggatg ggtttattta ttaaaaaaaa tttgacatta tttgacccac
      541 tgatctaaat ggacaggggc aaacacataa aagaagccaa aaatgtagcg gttgatgacg
      601 catgccggcc tgtggagcat atacacacat atgcggctcc gggcaacgca tcatacaccg
      661 gcaagttcgc tacaaaatag tcaggagcga tcatgaagca cacaacgaaa tacctatgca
      721 tagcgcttat cctcttactc gcgacagcgg tatcggcgca cagcattcaa gtcgattcca
      781 acgccatcca gcaatatcgc ggatcgctgg ggaaatggat acaggtggac tcgccgggca
      841 ccctgcatgc ctatgcgaaa aagttttcgg tcgaccccaa agacgtctat acgattaatg
      901 ggaccaacgg gtacgggccc ctcgtcaata attatgtctt ttttccctac tcgaaggagt
      961 atctcaagga gctcgaatcc aagggcatta cgcggcaaac cgtcgaggac caggccgaca
     1021 agtttttctg gcccatcagg accttcgggc acatctcgtc tgtactcggg aaccgctggg
     1081 ggcgctttca tccaggcgtg gacatcccct cgacgcgcga gaccccgatc catgccgctc
     1141 agagcggacg cgtgatctac gcccgctact gcggcggata cggcctcacc gtcgccatcg
     1201 agcacaagaa caatctcatg acccgctatg cgcacaactc ggtcaacctc gtcaaggagg
     1261 gcgacttcgt gcaaaagggg cagatcatcg cccttgtggg cagcaccggg aactcgacgg
     1321 ggaaccacct tcacttcgag gtgcgctgca acgacatccc gctcgatccg ctcgattttc
     1381 tcccgcgcat cgagaacatt caaaagcccc actcgattaa aagctggaag tagttcccgt
     1441 ccgctcgcat gataacaaac ggcgccaatg gcgccgtttt tgttttccaa ggtcaagtga
     1501 ttgacgcaaa tcctcgtgcg gcctcatcat ggcgcacgag gaccgcgatg acagatttcg
     1561 atacaccgat cgacctgcac attcactcaa gcttcagcga ggacgccgac taccccgtcg
     1621 atgcgatctt cgccatggcg cacgagtcgg gcgtgtccgt catctccatc accgaccatg
     1681 attccgtcga gtccataccc gtcgccctga gcctcaccgg cgcacaccct gtccggtacg
     1741 ttcccggggt ggagctcacc accatgttcc cgcgcgacgg ctcacaacag catgttctgg
     1801 gttatttcgt cgatcccgac aaccgccgcc tccaggagac cctgaccgg
//
LOCUS       DAORHF010000441         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_84706_length_1809_cov_5.252566, whole genome shotgun sequence.
ACCESSION   DAORHF010000441 DAORHF010000000
VERSION     DAORHF010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84706_length_1809_cov_5.252566"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..>1809)
                     /gene="rseP"
                     /locus_tag="PKJ16_19560"
     CDS             complement(236..>1809)
                     /gene="rseP"
                     /locus_tag="PKJ16_19560"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HNV49245.1"
                     /translation="TITYILAAVVLLGLCIFIHELGHLLGGRMVGIRAEIFSIGYGKG
                     VLKKKIGDTTYQLTLIPFGGYCKFYGDEPGESRTGESYEFLSAHPLRRIVTVVMGPLF
                     NLFFGIVLFFIMNLVGYPVESSRIAIPDFFKSGAYVAPAHTAGLMTGDRIVEISGKRI
                     TGFSDIQSNVVFSKGEPLDVAVERDGKILRFPVTPSRVSENDYFTIGVMPYGERVLVV
                     DSLENEPAARAGIEKLDEVKALDGVPVKTPADFVAYIRSHPEKRVVVSLVRRGKSMDV
                     TVAPRYREELALAGFEDGRFKGTSIEFSITDPDKIKIIKAAIGAKKVRVNDRTVSTYD
                     EFLSVLAQNRGKRVRIYNGAEEYSASVEYKKYGFIGVETAIAPEMVTVRHSLPMAAAR
                     AIVDPVDFIVMNLKGLGMLITGKLDVRKNLSGPVRIAKIAGDTAHYRGISSFIILMAK
                     ISIILMVMNLLPIPVVDGSHIIFYLIEMIKGSPINEKVMEKIQFVGVALLIMLGVFVV
                     FNDLSFFPFFQKLFN"
BASE COUNT          379 a          555 c          521 g          354 t
ORIGIN      
        1 gattctcctg agcaccgggg tcaacaggag aatcttttcc aggcgggacc gtactgtcgt
       61 attctgcatt gccacctgcc aatcggattc gtgcgttcgc tgcggtcgcc ctattgcact
      121 cgctccgccg caaaacaatc atcgtgattt tgcggctcac ggcggacatc ctgtccgcct
      181 gctcgctgca acagggcgac cgcagcgaac ttgagtcgga tgccaaatga atacactagt
      241 taaacagctt ctgaaaaaac gggaaaaagc tcaagtcatt gaacaccacg aacactccca
      301 gcatgatcag gagcgccacg ccgacgaatt gtatcttctc catcaccttt tcgttgatgg
      361 gactgccctt gatcatctcg atgagataga agatgatgtg gctcccgtcg accaccggga
      421 tgggcagcag gttcatcacc atgagaatga tggatatctt cgccatgagg atgatgaacg
      481 acgagatgcc gcgatagtgc gcggtgtcgc cggcgatctt cgcgatgcgc accggccccg
      541 acaggttttt acgcacatcg agcttgccgg tgatgagcat tcccagtccc ttgagattca
      601 tgacgatgaa atcgaccggg tccacgatcg cccgcgcggc cgccatgggg agcgaatggc
      661 gcacggtgac catttccggc gcaatggcgg tctcgacgcc gatgaagccg tacttcttgt
      721 actcgaccga cgcggaatac tcctccgcac cgttatagat gcgaacgcgc ttcccgcggt
      781 tttgcgccag cacggagagg aactcatcat acgtcgacac ggtccggtcg ttcacgcgga
      841 ccttcttcgc cccgatggcc gccttgatga tcttgatctt gtccggatcg gtgatgctaa
      901 attcgatcga cgtgcccttg aaacgaccat cctcaaaccc ggcgagggcc agttcctccc
      961 ggtagcgggg cgcgactgtg acatccatcg acttgccgcg acgcaccagc gaaaccacga
     1021 cgcgcttctc gggatgcgag cggatgtacg caacgaagtc cgccggcgtt tttaccggca
     1081 cgccgtcgag cgccttcacc tcgtcgagct tttcgatgcc ggcccgcgcg gccggctcgt
     1141 tctcgagcga gtcgaccacc aggacgcgct ccccgtaggg catcaccccg atggtgaaat
     1201 aatcgttctc ggagacccgc gacggcgtta cgggaaagcg caggattttc ccgtcgcgct
     1261 cgacggcgac atcgaggggt tcgcctttcg agaacaccac gttcgactgg atgtcggaga
     1321 agcccgtgat gcgctttccg gatatctcaa cgatccggtc gcccgtcatg aggcccgccg
     1381 tatgcgccgg cgcgacatag gcgccggact tgaagaagtc cgggatggcg atgcggctcg
     1441 actcgaccgg atagcccacc aggttcatga tgaaaaagag cacgatgccg aagaacagat
     1501 tgaagagcgg acccatcacc acggtcacaa tgcgcctgag cggatgcgcc gagaggaact
     1561 cgtaggattc gcccgtccgc gactcgcccg gctcgtcgcc gtagaacttg cagtagccgc
     1621 cgaaggggat caacgtgagc tgataggtgg tgtcgccgat cttcttcttg aggacgccct
     1681 tgccgtagcc gatggagaag atctcggccc tgatgccgac cattcggccg cccagcaggt
     1741 gccccagctc gtgaatgaag atgcacagtc ccaacaggac caccgccgcg agaatatagg
     1801 ttatcgtta
//
LOCUS       DAORHF010000442         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_85831_length_1795_cov_4.105747, whole genome shotgun sequence.
ACCESSION   DAORHF010000442 DAORHF010000000
VERSION     DAORHF010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85831_length_1795_cov_4.105747"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="PKJ16_19565"
     CDS             <1..1052
                     /locus_tag="PKJ16_19565"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HNV49246.1"
                     /translation="GPFASLYHSGVMLCLVFTFAFVRLRFRHATASGVLVGAGYIIPH
                     ALIGDLPGDQLAHNAMNLLGTIGVGMCIAYLNEYHLRYSFVQELVIGEKSRTLEAQNA
                     LIDHELEIAKTIQEQYLPASGVHGRIGALYRPMDRVGGDFFDIITFADPDEIGVFISD
                     VSGHGVPAALYTSMLRVALTQAGDLQRDPQGLLGHLDRALLPHLKSAFITCFYGVLNM
                     RSGDFRYANAGHLAPLIVSADSIEPLPCPPSVPLAAFSESTVRRHERDFSNRAVTLPT
                     GSRLLLFTDGLTEARNRFRPAEMFEYAGMHDALRSAGAMPVGAFIDRLFEALVGFRGG
                     DSFEDDICILCLEVG"
     gene            1055..>1795
                     /locus_tag="PKJ16_19570"
     CDS             1055..>1795
                     /locus_tag="PKJ16_19570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49247.1"
                     /translation="MPNGMNTGLYVGRYYTEGELARAVRRLSFPAPLEEAFLAYYHEK
                     TVLFIRIAGLFGVLLYLANITTDFNSFPGLEGVSLAIRIGYSLFASMAVVITFFPALR
                     SILQFYLFLVMVTGSAGIAAFTYLGTGIYASLYHSGIMLVLVFTFAFVRLRFRPASLA
                     GLVIGVGYVVPHAVIGDLPANQLSHNAMNLVGTIAVGMCIAYLNEYHLRDSFVKGLII
                     DEKSRSLESRTRQIDREMEIAKTIQEHFL"
BASE COUNT          270 a          617 c          546 g          362 t
ORIGIN      
        1 ccggcccctt cgcgagcctc tatcacagcg gggtgatgct gtgcctcgta ttcaccttcg
       61 ccttcgtgcg gctgcgcttc cgccacgcga cggcctccgg cgtcctggtc ggcgccggct
      121 acatcatccc ccatgcgctt atcggggacc ttcccgggga ccagctggcg cataacgcca
      181 tgaacctcct ggggacgatc ggggtgggca tgtgcatcgc ctacctcaac gagtaccatc
      241 tccgctactc gttcgtccag gagctcgtca tcggcgaaaa gagccggacg ctcgaggcgc
      301 agaacgccct catcgatcac gagctcgaga tcgcgaaaac gatccaggag cagtacctgc
      361 ctgcctccgg cgtccacggt cgcatcggcg ccctgtaccg cccgatggac cgcgtgggcg
      421 gcgatttttt cgacatcatc accttcgccg atcccgacga gatcggcgtg ttcatcagcg
      481 atgtgtccgg ccacggcgtc cccgcggccc tctatacctc catgctccgc gtggccctga
      541 cccaggccgg cgacctgcag cgcgatccgc aggggctcct ggggcacctg gaccgcgccc
      601 ttctcccgca cctgaagagc gccttcatta cctgcttcta tggtgtgctg aacatgcgct
      661 cgggcgactt ccggtacgcc aatgcggggc acctggcccc gctgatcgtg agcgccgatt
      721 cgatagaacc gttgccctgc ccgcccagcg tgccgcttgc ggcattctcc gaatcgaccg
      781 tccgccggca cgaacgggac ttttcgaacc gcgcggtcac gctgccgacc ggttcgcggt
      841 tgctcctgtt caccgacggg ctcaccgagg cgcgcaaccg cttccgtccc gccgagatgt
      901 tcgagtatgc ggggatgcat gatgcgctcc ggagcgcggg cgccatgccg gtgggcgcgt
      961 tcatcgaccg cctgttcgag gcgctggtcg ggtttcgggg cggcgactcc ttcgaggatg
     1021 atatctgcat cctctgcctc gaggtgggct gatcatgccc aacgggatga acacggggct
     1081 ctatgtcggc cgctattaca ccgaaggcga gctcgcgcgc gccgtgcggc ggctctcgtt
     1141 tcccgcgccg ctcgaggagg cctttcttgc ttactatcat gagaaaaccg tgctgttcat
     1201 acgcatcgcg ggcctgttcg gcgtgctgct gtacctggcg aacatcacca ccgatttcaa
     1261 ttccttcccc ggcctggagg gcgtctccct cgccatccgg atcgggtaca gcctgttcgc
     1321 ctcgatggcg gtggtgatca cgtttttccc ggcgctcaga tcgattcttc agttctatct
     1381 cttccttgtc atggtgaccg gcagcgccgg catcgcggcc ttcacctacc tgggcacggg
     1441 gatctacgca agcctgtatc acagcggcat catgctggtg ctggtgttca ccttcgcctt
     1501 cgtgcggctg cgcttccgcc cggcgtcgct ggccgggctc gtcatcggcg tggggtacgt
     1561 cgttccgcac gccgtcatcg gcgatctgcc ggcgaaccag ctttcgcaca acgccatgaa
     1621 cctcgtgggg acgatcgcgg tgggcatgtg catcgcctac ctgaacgagt atcacctgcg
     1681 ggactcgttc gtcaaggggc tcatcatcga cgagaagagc cggtcgctgg agagccgcac
     1741 gcgtcagatc gaccgcgaga tggagatcgc gaaaacgatc caggagcact tcctg
//
LOCUS       DAORHF010000443         1788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_86415_length_1788_cov_2.829198, whole genome shotgun sequence.
ACCESSION   DAORHF010000443 DAORHF010000000
VERSION     DAORHF010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86415_length_1788_cov_2.829198"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1788
                     /gene="carB"
                     /locus_tag="PKJ16_19575"
     CDS             <1..>1788
                     /gene="carB"
                     /locus_tag="PKJ16_19575"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007358665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HNV49248.1"
                     /translation="CKALKEEGYKVILVNSNPATIMTDPEFADVTYIEPLTVEILEKI
                     IAKERPSALLPTLGGQTALNLSMSLHKAGILEKYDCEMIGAKPPAIEMGEDRNLFKEA
                     MLRIGLDVAKSGVVHSLEEARLAAEKIGTFPLIIRPSFTLGGTGGGIAYNREEFEQIV
                     NGGLEASPTHEVLVEECLLGWKEYEMEVMRDHKDQCVVICSIENFDPMGVHTGDSITV
                     APAMTLTDKEYQVMRDASFAVIREVGVETGGSNIQFSVDPKTGRMVVIEMNPRVSRSS
                     ALASKATGFPIAKIAAKLAVGYSLDELRNDITRLTPASFEPTIDYVVTKIPRFTFEKF
                     PDADPTLTSSMKSVGEAMAIGRTFKESLQKALRSLEIGARGFGGGGKFGTDDLPDMQT
                     IQAKLATPNAERIFYIRYAFMAGLTIDDVFELTQIDPWFLHQLHEIFEMEQQLRKQVL
                     TLIDTDLLRRAKKFGFSDAQLAYIFKTDLAAVRAERKKRKIDTTYRLVDTCAAEFEAF
                     TPYYYSSYGDENEVIPSSRRKIMILGGGPNRIGQGIEFDYCCVHASFALRENGFETVM
                     VNSNPETVSTDYDTSDRLYFEPLTLEDVL"
BASE COUNT          356 a          618 c          495 g          319 t
ORIGIN      
        1 cgtgcaaagc cctcaaggag gagggttaca aggtcatcct cgtgaactcc aacccggcca
       61 cgatcatgac cgatccggag ttcgccgacg tcacctacat cgagccgctc acggtcgaga
      121 tcctggaaaa aatcatcgcc aaggagcgcc cctccgcgct gctgcccacg ctcggcggcc
      181 agaccgcgct caatctctcg atgtccctgc acaaggccgg cattctggag aaatacgact
      241 gcgagatgat cggcgccaag ccgcccgcga tcgagatggg cgaggatcgc aaccttttca
      301 aggaggctat gctccgcatc ggcctcgatg tcgccaagtc cggcgttgtc cattcgctgg
      361 aggaggcccg cctcgcggcc gagaagatcg gcaccttccc gctcatcatc cgcccgagct
      421 tcacgctcgg cggcaccggt ggcggcatcg cctacaaccg cgaggaattc gagcaaatcg
      481 tcaacggtgg cctcgaggcc tcgccgaccc acgaggtgct cgtcgaggaa tgtctcctcg
      541 gctggaagga atacgagatg gaggtcatgc gcgaccacaa ggaccagtgc gtcgtcatct
      601 gctcgatcga gaatttcgac ccgatgggcg tccacaccgg cgactccatc acggtggccc
      661 cggcgatgac gctgacggac aaggaatatc aggtcatgcg cgatgcctcc tttgcggtca
      721 tccgcgaagt cggcgtcgag accggcggct ccaacatcca gttctccgtc gatccgaaga
      781 ccggccgcat ggtcgttatc gagatgaatc cccgcgtgtc gcgctcctcg gcgctcgcgt
      841 ccaaggccac cggcttcccg atcgccaaga tcgcggccaa gctcgccgtc ggctactcgc
      901 tcgacgaact gcgcaacgat atcacgcgcc tcactcccgc ctccttcgaa ccgacgatcg
      961 actacgtcgt cacgaagatc ccgcgcttca cgttcgagaa gttcccggat gccgatccca
     1021 cgctgacgtc gtccatgaag tcggtcggcg aagcgatggc catcggccgc accttcaagg
     1081 agtctctgca aaaggcgctt cgctctctcg aaatcggcgc ccgcggattt ggcggcggcg
     1141 gcaagtttgg gactgacgat ctgcccgaca tgcagaccat ccaggccaag ctggcgacgc
     1201 ccaacgccga gcgcattttc tacattcggt acgccttcat ggcgggcctc acgatcgatg
     1261 atgttttcga gctcacccag atcgatccgt ggttcctcca ccagctccat gagattttcg
     1321 aaatggagca gcagctccgc aagcaggtcc tcaccctcat cgataccgac ctgctccgcc
     1381 gcgcaaagaa gtttggcttc agcgatgccc agctcgcgta catcttcaaa accgacctcg
     1441 ccgccgtgcg cgccgagcgc aagaagcgca agatcgacac cacctaccgc ctggtcgaca
     1501 cctgtgcggc tgagttcgag gccttcacgc cctactacta ttcgagctat ggcgatgaga
     1561 acgaggtcat cccttcgtcc cgccgcaaga tcatgatcct cggtggcggc cccaaccgca
     1621 tcggccaggg catcgagttc gactactgct gcgtgcacgc cagcttcgcc cttcgcgaaa
     1681 acggcttcga gaccgtgatg gtcaactcga accccgagac cgtttcgacc gactacgaca
     1741 ccagcgaccg cctctacttc gagccgctca cgctggaaga tgtcctcg
//
LOCUS       DAORHF010000444         1776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_87320_length_1776_cov_4.254503, whole genome shotgun sequence.
ACCESSION   DAORHF010000444 DAORHF010000000
VERSION     DAORHF010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87320_length_1776_cov_4.254503"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PKJ16_19580"
     CDS             <1..225
                     /locus_tag="PKJ16_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HNV49249.1"
                     /translation="RLEVRGHTDSVGDPRYNKRLSERRADAVIEYMVKNGISPERLSA
                     VGMGSEKPIASNTTPEGRRKNRRTEFFFKN"
     gene            complement(230..646)
                     /locus_tag="PKJ16_19585"
     CDS             complement(230..646)
                     /locus_tag="PKJ16_19585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49250.1"
                     /translation="MKRLLLVDDNEKYAAIITNHFRPLGYEIDHAVSAADGLSMFTKN
                     GLDHYALIVTDITMESQVAGLFMLRKIHTMGYRGPIVIASTGFDFPGAKTLSKLFLRP
                     TGATYLVPKTTVLRNDFEYHPMAFFAKPLKAFPESS"
     gene            complement(746..1498)
                     /locus_tag="PKJ16_19590"
     CDS             complement(746..1498)
                     /locus_tag="PKJ16_19590"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HNV49251.1"
                     /translation="MNRKARIENTVETRSRILQSAKKEFAERGYDGARMGSIAKRARA
                     NQALIHYYFGNKENLYREVLDRIFGIGTFKERGFDSLIAEWGLSPRESLYVVIYFFVH
                     FHFDASDNDFNKIIIREIVEERATLKAILREYVIPWHEAIERIIVRGIEAGEFETANP
                     VLMVYNVFNFIFSYINSRHCFEGTEWHHRLFSHSDVQQFLQFTLDYVFKALQVPGAKV
                     RPVRLPPGIIASVDEIIRECKKLSIAEGAPGS"
     gene            complement(1577..>1776)
                     /locus_tag="PKJ16_19595"
     CDS             complement(1577..>1776)
                     /locus_tag="PKJ16_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HNV49252.1"
                     /translation="HELAGYFMEFSRYHDACSFRPCTHDHEPQCAVRDMVDEGAIAHE
                     RYISYLNILATLREYRAIKYG"
BASE COUNT          386 a          497 c          508 g          385 t
ORIGIN      
        1 cggctcgagg tgcgcggcca caccgactcg gtgggcgacc ctcgctacaa caaacgcctg
       61 tccgagcgtc gcgcggatgc cgtgatcgaa tatatggtga aaaacgggat cagcccggag
      121 cgcttgagcg cggttggcat gggatcggaa aagccgatcg catcgaatac gacacccgag
      181 ggccggagaa aaaatcgacg gaccgaattc ttctttaaga attgaagcgc tacgacgact
      241 cgggaaacgc cttgagcggt ttcgcgaaaa acgccatcgg atgatactcg aaatcgttcc
      301 tgagcacggt cgtcttcggg acgaggtacg tggcgcccgt gggccgcaga aacagcttcg
      361 acaacgtctt tgcgcccggg aaatcgaatc cggtgctcgc aatgacgatg ggtccacgat
      421 agcccatcgt atggatcttc cgcagcataa agagcccggc gacctgcgat tccatcgtga
      481 tatcggtcac gatcagcgca tagtgatcga ggccgttttt cgtgaacatc gaaagcccat
      541 cggcggcgct cacggcgtgg tcgatctcgt acccgagcgg tcggaaatgg ttcgttatga
      601 tggccgcgta tttttcgttg tcgtcaacca gtagcaatcg cttcatgagc ccgcctgccc
      661 gtcattgttc gcccgcaacc agcgggtcgg gacgatatcg tgtattcacg cctatttatc
      721 aaggattttt tcttgaaccg gattgttatg atcccggcgc gccttcggcg atcgagagct
      781 ttttgcattc gcgtatgatc tcgtcaaccg acgcgatgat cccgggcggg aggcgcaccg
      841 gccgcacctt tgcgccggga acctggagcg ccttgaacac gtaatccaga gtaaactgca
      901 aaaactgctg cacgtcgctg tgtgaaaaca accgatggtg ccattcggtt ccctcgaaac
      961 agtgacggct gttgatgtag ctgaagatga agttgaagac gttgtacacc atgagcaccg
     1021 ggttcgccgt ctcgaactcc cccgcctcga tgccgcgcac gatgatccgc tcgatcgcct
     1081 cgtgccacgg aatgacatac tcgcgaagga tcgccttcag tgtggcgcgc tcctccacga
     1141 tttcgcgtat gatgattttg ttgaaatcgt tgtcggacgc atcgaagtga aaatgcacga
     1201 aaaagtatat caccacgtac aggctctccc gcggagaaag tccccattcg gcgatgagcg
     1261 aatcgaaccc gcgctccttg aacgtcccga tcccgaaaat tcgatcgagg acctcccggt
     1321 agaggttctc cttgttcccg aaataataat ggataagggc ctgattcgcc cgagcccgtt
     1381 ttgcgatgga acccatccgc gcgccgtcgt atccgcgctc ggcgaactcc tttttcgctg
     1441 attgaaggat tcgggagcgc gtttcaaccg tgttttcgat gcgggctttt cgattcatgg
     1501 cggcgtttga cattccatgc tgcggcgcta tcgcgttacc gtgtactcgc gacgcgcgca
     1561 gctgtcaatg gaataatcat ccatatttga tcgcgcggta ctcccggagc gtggcgagaa
     1621 tgttcaggta ggaaatatag cgctcatgtg cgatggcgcc ctcgtcaacc atgtcccgca
     1681 cagcgcactg cggttcatga tcgtgcgtgc agggacggaa ggaacaggcg tcatggtaac
     1741 gggaaaactc catgaagtag ccggcaagct catggg
//
LOCUS       DAORHF010000445         1770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_87829_length_1770_cov_3.683382, whole genome shotgun sequence.
ACCESSION   DAORHF010000445 DAORHF010000000
VERSION     DAORHF010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87829_length_1770_cov_3.683382"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="PKJ16_19600"
     CDS             <1..974
                     /locus_tag="PKJ16_19600"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HNV49253.1"
                     /translation="NMADWPLLQSLIGAANAFIVCAVGFMRELPFALVLRNVELSFAN
                     GVVNAILALGIIPVYENLFGISTRFKLLELSDLNAPIFKRMLIRAPGTYNHSLMVANM
                     AEAACKDIGANALLARVGGYYHDIGKINDSGIYIENRVTDPRVKTFSPLEYSRLIISH
                     VEKGVVLAREQGLPDSIVDFIREHHGKSVMTYFYHQALEAVEADGPDSVRKGDFQYPG
                     PPPSSRESAVVMLADAIEAASRSINEPNKAKLEGMVKKIIFNRLNDGDLEFSELTMSE
                     LNKIQRSFLRILFGIFHTRIEYPEKSDVARLEEKIQKKENGPAGNGE"
     gene            964..1422
                     /gene="ybeY"
                     /locus_tag="PKJ16_19605"
     CDS             964..1422
                     /gene="ybeY"
                     /locus_tag="PKJ16_19605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HNV49254.1"
                     /translation="MANSVSICSENVRFPYRRIRKKDIDRWLRAVCRAAFPKGATVTL
                     IVCDNAAIRIINKKYRKKDRPTDVITFAYDESPFPARGTQETSGDIYLSLERAAENAA
                     EYGVTLADEIQRLLVHGVLHMAGYDHERSVAEERRMRKREEEVLRGLRVR"
BASE COUNT          363 a          510 c          551 g          346 t
ORIGIN      
        1 cgaacatggc cgattggccg ctcttgcaga gcctgatcgg cgcggcgaac gcgttcatcg
       61 tgtgcgcggt cggctttatg cgggagctgc cgttcgcgct ggtgctgcgc aacgtggagc
      121 tctcgttcgc caacggcgtg gtgaatgcga tcctggcgct gggcattatc cccgtgtacg
      181 agaacctgtt cggcatttcc acgcggttca agctcttgga gctttcggac ctcaacgccc
      241 ccatcttcaa gcggatgctc atccgcgcgc cggggacgta taatcactcg ctcatggtcg
      301 cgaacatggc cgaggcggcc tgcaaggaca ttggcgcgaa cgccctcctg gcgcgcgtgg
      361 gcgggtatta tcacgacatc ggcaagatca acgattcggg catctacatc gagaaccgcg
      421 tgaccgaccc gcgcgtgaag acgttttcgc cgttggagta ttcgcggctc atcatatcgc
      481 acgtggagaa gggggtcgtg ctggcgcgcg agcagggatt gccggactcg atcgtcgact
      541 ttattcgcga gcaccacggg aagtcggtga tgacctactt ctaccatcag gcgcttgagg
      601 cggtcgaggc cgatggcccg gactccgtcc ggaagggcga tttccagtat ccggggcccc
      661 cgcccagctc tcgcgagtcg gcggtggtga tgctggccga cgcgatcgag gccgcctcgc
      721 gctccatcaa cgagcccaac aaggcgaagc tcgaggggat ggtgaagaaa atcatcttca
      781 accgccttaa cgacggcgac ctggagttct cggagctcac gatgtcggag ctcaacaaga
      841 tacagcgctc gttcctgcgc atcctcttcg gcattttcca cacgcgcatc gaatacccgg
      901 agaagtccga tgtggcccgc ctcgaggaga agatccagaa gaaggaaaac ggaccggcgg
      961 gaaatggcga atagcgtttc catctgcagc gagaatgtcc gcttcccgta tcgtcggatc
     1021 aggaagaagg acatcgaccg ctggctgcgc gccgtctgcc gcgcggcgtt cccgaagggc
     1081 gcgacggtca cgcttatcgt gtgcgacaac gcggccatca ggatcatcaa taaaaaatac
     1141 cgcaagaagg accgccccac cgacgttatc accttcgctt acgacgaaag cccatttccc
     1201 gcgcgcggaa cgcaggaaac gagcggcgac atctatcttt cattggagcg cgccgccgag
     1261 aatgccgcag agtacggcgt gacgctggcc gacgagatac agcgcctgct ggtgcacggc
     1321 gtgttgcaca tggccgggta cgatcacgag cgctcggtcg cggaggagcg gcgcatgcgg
     1381 aagcgggagg aagaggtgtt gagagggttg cgagtgcgtt gagtatgtat ttcgatagta
     1441 tacatcctcg atatccagca ataaaccatg taattgtgca atagtattga agaattgcat
     1501 ggttgtatat ggaatacggg acgcgttttc ttgaaaaccg ttatgaatga attatcctcc
     1561 gaacgggaca tccccttcgg catagtgctc tacggcggga atgaggccct gccgatcacc
     1621 gagaagattg tcgccatccc ggcgtcggcg gttctgtagg ggggtggtgc cgctaaaggc
     1681 tcagcacgta gaacagctgt cgtcgccgca gccgcagccg cattctccgt agccgtactt
     1741 cgcctcgtcg ttcacctcga gcagcttgat
//
LOCUS       DAORHF010000446         1751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_89351_length_1751_cov_6.102594, whole genome shotgun sequence.
ACCESSION   DAORHF010000446 DAORHF010000000
VERSION     DAORHF010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89351_length_1751_cov_6.102594"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PKJ16_19610"
     CDS             complement(<1..498)
                     /locus_tag="PKJ16_19610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HNV49255.1"
                     /translation="MPRSPVTVATRYRITEAIGEGGISTVYRAVDEAENGAVVAIKLL
                     KEAIASKRIEDIIRFQAEAVTVSRLEHPSVIRIHDTGEHEGRHYLVMDLVDGRSLAAI
                     MAADDPPTVGEAVFIVRTIAQALDYIHSSGVIHRDIKPGNILVPRGENGHDFARLVII
                     DFGLAQ"
     gene            complement(515..>1751)
                     /locus_tag="PKJ16_19615"
     CDS             complement(515..>1751)
                     /locus_tag="PKJ16_19615"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HNV49256.1"
                     /translation="YAGAGGSSLSSFAKRLLFAISFRDINLLISSNVTAESRVLFRRN
                     IGEMVRTLTPFLEFETDPYVVLSEGRLFWIIDAYTTSNRFPYATPVRTDSGRINYIRN
                     SVKVVIDAYNGSMSYYLSDPADPIVRTYANIFPGLFKKLDEMPAGLRAHIRYPEAIFN
                     IQCRILLRYHMTRADIFYNNDDAWDIPRQIYDDSEEPVHSYYIVTTLPGEKKSEFILV
                     MPFTPVKKDNMIGFLIARCDVPNYGALTLYMLPKDKLSYGPMMIERRIKQDADISKQL
                     ALWSGKGSRVIRGNMLFVPIEESLIFVEPLYLKAESNESREMPELKRVIVSFADRITM
                     GENLNSAMEQLFTGGGFSIGEGASSGTARERLRDLADKAQFHLNEAERYQREGDWARY
                     GEELKKMKAVLNNMKGVRE"
BASE COUNT          288 a          514 c          616 g          333 t
ORIGIN      
        1 ctgcgcgagg ccgaaatcga tgatgacgag ccgggcgaag tcgtgaccgt tctcgccgcg
       61 cgggacgagg atgttgccgg gcttgatgtc gcggtggatc acgccgctcg agtggatgta
      121 gtccagcgcc tgcgcgatgg tccgcacgat gaacacggcc tcgcccacgg tgggcgggtc
      181 gtccgccgcc atgatggccg cgaggctccg gccgtccacg aggtccatga ccaggtagtg
      241 ccggccctcg tgctcgccgg tgtcgtggat cctgattacg gacgggtgct cgaggcgcga
      301 caccgtgacg gcctccgcct ggaagcggat gatgtcctcg atgcgcttgg aggcgatcgc
      361 ctccttgaga agcttgatcg cgacgaccgc gccattctcc gcctcgtcaa cggcgcggta
      421 gaccgtgctg atgccgccct cgccgatggc ctccgtgatg cggtagcgcg tggcgactgt
      481 tacgggtgag cggggcatgg cgcgccgccg gggatcactc ccgaaccccc ttcatgttgt
      541 tgagcacggc cttcattttt ttgagctcct cgccgtagcg cgcccaatcg ccctcccgct
      601 gataccgctc cgcctcgttg agatggaact gcgccttgtc ggcaagatcc cgcaggcgct
      661 cgcgggcggt gccggacgag gcgccctcgc cgatcgagaa gccgccgccc gtgaagagct
      721 gctccatcgc cgagttgagg ttctcgccca tggtgatgcg gtcggcgaag gagacgatca
      781 cgcgcttgag ctcgggcatc tcgcgcgact cgttcgattc ggccttgagg tagagcggct
      841 ccacgaagat gagcgattcc tcgatgggaa cgaagagcat gttgccgcgg atcacccgcg
      901 agcccttgcc gctccacaac gcaagctgct tggagatgtc ggcgtcctgc ttgatgcgcc
      961 gctcgatcat catggggccg taggagagct tgtccttggg aagcatgtag agcgtgagcg
     1021 cgccgtagtt gggcacgtcg cagcgcgcga tgaggaagcc gatcatgttg tccttcttga
     1081 cgggcgtgaa gggcatcacg aggatgaact cgctcttctt ctcgcccggg agcgtggtca
     1141 cgatgtagta gctgtggacg ggctcctcgg agtcgtcgta gatctgccgg gggatgtccc
     1201 aggcgtcgtc gttgttgtag aagatgtccg cgcgggtcat gtgatagcgc aggaggatgc
     1261 ggcactggat gttgaagatc gcctccgggt agcgtatgtg ggcgcgcaac ccggccggca
     1321 tctcgtcgag cttcttaaag aggcccggga atatgttcgc ataggtgcgc acgatggggt
     1381 cggccggatc gctcaggtag tagctcatgg agccgttgta ggcgtcgatg accaccttca
     1441 cggagttgcg gatgtagttg atgcgcccgg agtcggtgcg cacgggcgtc gcgtacggga
     1501 agcggttcga ggtggtgtag gcgtcgatga tccagaagag gcgcccctcg gagagcacca
     1561 cgtaggggtc ggtctcgaac tccaggaacg gcgtgagcgt gcgcaccatc tccccgatgt
     1621 tgcggcggaa gaggacgcgg ctctcggctg tcacgttgga cgaaatgaga aggttgatgt
     1681 cgcggaacga tatcgcgaac agcagccgct tcgcgaacga cgagagtgac gatccgcccg
     1741 ccccggcgta g
//
LOCUS       DAORHF010000447         1749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_89513_length_1749_cov_4.757969, whole genome shotgun sequence.
ACCESSION   DAORHF010000447 DAORHF010000000
VERSION     DAORHF010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89513_length_1749_cov_4.757969"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PKJ16_19620"
     CDS             complement(<1..319)
                     /locus_tag="PKJ16_19620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein"
                     /protein_id="HNV49257.1"
                     /translation="MPEQAVLNVDLADVWKERGRKQYIRTIAWGDEITVVKQAATYLE
                     VTITFFREKADGSILPESVTGYIEPTKSSGIKVADVVRPVADNKVLKVNFVDVQQGDG
                     MVIE"
     gene            379..993
                     /locus_tag="PKJ16_19625"
     CDS             379..993
                     /locus_tag="PKJ16_19625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV49258.1"
                     /translation="MIVGDRIRRSSGEITPASLLPMESPACYICAMTMQFQKKDPRAT
                     ISTPFGEIRIRFYPDDAPRHVENFINLAKMGFYDDTTFHRVVPGFIIQGGDPLSKTPD
                     RLSHGTGGPGYFLNPEPNDRPHKRGAISMAKMPRDGNSTRDFNDNGSQFFISLQDNGG
                     LDRRYSIFGEIIRGIEVIDMIAKMPCDERDNPLEPIRMKVTVKE"
     gene            1166..1465
                     /locus_tag="PKJ16_19630"
     CDS             1166..1465
                     /locus_tag="PKJ16_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49259.1"
                     /translation="MTEQYPSYPEGVRWAIDKGNILRGMTEDQVYLALGTPVCKKDVE
                     DEGRTVTVWLYPPIGRNACITSAFRVYFEQGAVTTWDRYTTPTRYTDPVGGVPAY"
BASE COUNT          341 a          535 c          493 g          380 t
ORIGIN      
        1 attcgatgac catgccgtcg ccctgttgga catcgacgaa gttgaccttg aggaccttgt
       61 tgtccgccac gggccggacc acatcggcca ccttgatgcc tgaagatttg gtcggttcga
      121 tataaccggt gacggattcg gggaggatgc tgccgtcggc tttttcgcga aagaacgtga
      181 tcgtgacttc aaggtaggtg gcggcctgtt tgaccaccgt gatttcatcg ccccaggcaa
      241 tcgtccggat gtattgtttg cggccacgtt ctttccacac atcggccaga tcgacgttga
      301 gaacggcttg ttcaggcatc acatctcccc ttgcaggcgg tagcggctgg tctcacgctg
      361 gtgcattgcg gcggtccgat gattgtcggc gacagaatac gccgttcatc gggagaaatc
      421 acgccggcct ccctcttgcc gatggaatcg ccggcctgtt acatttgcgc catgactatg
      481 caatttcaga aaaaagatcc acgcgccacg atcagcacgc ccttcggtga aatccggatt
      541 cgtttttacc cggacgacgc cccgcgccat gtcgagaact tcatcaacct ggcgaagatg
      601 gggttttatg acgacaccac gttccatcgt gtggtgccgg gcttcatcat tcaaggcggt
      661 gacccgttga gcaaaacgcc ggaccgcctg tcacacggca ccggaggacc cggctacttc
      721 ctcaaccccg aacccaacga ccggccccat aagcgcgggg cgatttcgat ggcgaagatg
      781 cctcgcgacg gcaacagcac ccgcgacttc aacgacaacg gttctcagtt ttttatttcc
      841 ctccaggaca acggcggcct ggatcggcgc tattcgattt ttggcgaaat catccggggc
      901 atcgaggtca tcgacatgat cgcgaagatg ccgtgcgacg agcgcgacaa cccgctcgag
      961 ccgattcgta tgaaggtgac ggtgaaggaa tagtcgcctg cgcgaccacg cgtgggtgtg
     1021 tagtcgacga gtctgccacc tgatctcatc cattgtgacg atccccttcc ccttaccagg
     1081 tcggtcgctg atacgcagtg tgggaccttg cctgctgctc ctcaccgtct tgctgtcagc
     1141 ctgcgatggt cagcgatcac gcctgatgac ggagcaatac ccctcctatc ccgaaggcgt
     1201 gcgttgggcg attgataagg ggaacattct gcgcggcatg acggaagatc aggtgtatct
     1261 cgcgctcggc acaccggtgt gcaaaaagga tgtcgaggat gaagggcgga ccgtcacggt
     1321 ctggctctat ccaccgatcg gacgcaacgc ctgcatcact agcgctttcc gtgtctactt
     1381 cgagcagggc gccgtcacca cctgggatcg ctataccacg ccgacgagat ataccgatcc
     1441 ggtcggcggc gtccccgcct actagccggt tgttcacaaa ggccacccag catcgttctc
     1501 ggtcgctcat ctccctgcga cgtaccgcaa gggtacgcct cgtcccttcg ctcgctgcgg
     1561 ccgcgctggg cggacttttt gaccctcctg ttactccaag cggtgctccc ctgcgtgcgc
     1621 gccgacataa ccttcacgct tgacgcgcgg ggtgtatgac gatacgatga tcgatcagta
     1681 atcggtaagg aggtgctgca gtgtctatga ccactatctc gccgaagttc cagatcgtca
     1741 tccctaagg
//
LOCUS       DAORHF010000448         1742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_90090_length_1742_cov_4.448726, whole genome shotgun sequence.
ACCESSION   DAORHF010000448 DAORHF010000000
VERSION     DAORHF010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90090_length_1742_cov_4.448726"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="PKJ16_19635"
     CDS             <1..1006
                     /locus_tag="PKJ16_19635"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HNV49260.1"
                     /translation="DPAIEVVGTANNGQTAIYKNDTLDPDVITMDIEMPIMDGLESLR
                     TIIATKPKPVIMMSVLTQHGAEATFKAMEYGAVDFIPKPSSILSLTMDDISELLVSKV
                     KSVAGIVIKVPEAVAPPRADDAPRAKTPRRIEEVRRAAQPIKERSARVIAIGTSTGGP
                     SALFNVFKNLPHNLPNPVLVVQHMPEGFTKAFAERLNANSPLSVKEAADGDIVRPGWG
                     YIAPGHSHMRIENAGGERIIRVFTADKVSGHRPSIDVLFDSVAETCGAESVGVIMTGM
                     GKDGARGMLNIKRKGGYCVAQDEETSVVFGMNRVAVELGAVDDVVPLDEIPQKIVEHI
                     "
     gene            1158..1517
                     /locus_tag="PKJ16_19640"
     CDS             1158..1517
                     /locus_tag="PKJ16_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49261.1"
                     /translation="MARILIVDDAKFMRTLVRDALIPKGHEIIGEAENGVEAVELYKK
                     LKPDLVTMDITMREKDGIEAAEEILNFDKAARIIMVTALGQENLLTKAIKIGVKDFVV
                     KPFPPERLQKAAEKALS"
BASE COUNT          378 a          486 c          579 g          299 t
ORIGIN      
        1 ggatccggcg atcgaggtgg tgggcaccgc gaacaacggg cagaccgcga tttacaaaaa
       61 cgatacgctc gatcccgacg tcatcacgat ggacatcgag atgcccatca tggacgggct
      121 cgagtcgctg cgcaccatca tcgccacaaa gccgaagccc gtcatcatga tgagcgttct
      181 cacccagcac ggcgccgagg cgaccttcaa ggccatggag tacggcgcgg tcgacttcat
      241 ccccaagccc tcgtcgatcc tatccctgac gatggatgac atctcggagc ttttggtgag
      301 caaggtgaag tcggtcgccg ggatcgtgat aaaggtcccg gaggcggttg cgccgccgcg
      361 cgccgacgat gcgccgagag cgaagacgcc ccgccgcatc gaggaggtgc gacgggccgc
      421 gcagccgatc aaggagcgca gcgcgcgcgt gatcgcgatc gggacctcga ccggcgggcc
      481 ctcggcgctc tttaacgtgt ttaaaaacct tccgcacaat ctccccaatc cggtgctggt
      541 ggtgcagcac atgcccgagg ggttcaccaa ggcgtttgcg gagcggctca atgcgaattc
      601 cccgttgagc gtgaaggagg ccgccgacgg cgatatcgtc cgtccggggt gggggtatat
      661 cgcccccgga cactcccaca tgcgcatcga gaacgcgggc ggcgagcgga tcattcgggt
      721 ttttaccgcg gacaaggtgt cgggacaccg gccgtcgatc gacgtgctgt tcgattcggt
      781 ggccgagacc tgcggcgccg agtcggtggg cgttatcatg accgggatgg gaaaggacgg
      841 cgcgcgcggc atgctcaata tcaagcggaa gggcggctat tgcgtggcgc aggacgagga
      901 aacgtcggtg gtctttggca tgaaccgcgt ggccgtggag ctgggggcgg ttgatgatgt
      961 ggtcccgctc gatgagatac cccagaagat agttgaacat atatagggaa tggcgcgttg
     1021 ggaggcccgt gacgcggata aaatgaacaa aaattgcatt tcaggaaaat tttggttgat
     1081 cgcatatccg cagtatgtag gatactgccc agcgacgccc gatttccgga tttatctaaa
     1141 ctggctggag cgagaccatg gcgagaatac ttatcgttga tgacgcgaaa ttcatgcgga
     1201 cgcttgtgcg tgacgcgctc atcccgaagg gtcatgagat catcggcgag gccgagaacg
     1261 gggtcgaggc ggtggaactg tacaagaagc tcaagcccga tctcgtgacc atggacatca
     1321 ccatgcgcga aaaggacggt atcgaggcag ctgaggaaat cctcaatttc gacaaggcgg
     1381 cgcggatcat catggtgacg gcgctcggcc aggagaacct cctcaccaag gcgatcaaga
     1441 tcggcgtgaa ggatttcgtc gtgaagccgt tccctccgga gcgcctccag aaggcggcgg
     1501 aaaaagccct ctcctaagag cgaagcggac ggcgcggtcg cgcgccggga aaatcaacct
     1561 atcgacgtgc acgcgcgaag cgggtcccga ctcgcctgcg gcgcgtgtga aaacggtttg
     1621 gcatggaaca gcagacggac agggacagtg gcggcggtcg aacggatgcc tgcatcctgc
     1681 acgtcaacga tttcagcggg ccgctggacc tgctgtggga cctcatcaag cgcgcgaaga
     1741 ta
//
LOCUS       DAORHF010000449         1719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_92097_length_1719_cov_3.993990, whole genome shotgun sequence.
ACCESSION   DAORHF010000449 DAORHF010000000
VERSION     DAORHF010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92097_length_1719_cov_3.993990"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="pstA"
                     /locus_tag="PKJ16_19645"
     CDS             <1..581
                     /gene="pstA"
                     /locus_tag="PKJ16_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HNV49262.1"
                     /translation="KSPRIVKVIRLGINNLAGVPSVVFGLFGLSLFVVFLGFGTSILA
                     GSLTLGVLNLPVIIRSTEEALLVVPGTYREASLSLGATRWQTIYKIILPNALPGILTG
                     VMLSLGRAAGETAPIMFTAAAYYTPELPTSIFNEIMALPYHIYVMATAGTHIQATRHL
                     QYGTAIVLIALVLALNMTGLILRYRFRKRLKG"
     gene            complement(776..>1719)
                     /locus_tag="PKJ16_19650"
     CDS             complement(776..>1719)
                     /locus_tag="PKJ16_19650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV49263.1"
                     /translation="HSKRVLIANRAFLSLLDIRAAVFGRAYYEVVRGSSFNAKIEYSL
                     ARGVEARFIEALASGRYCEVFISPITEEKAVQGILIVLHDITEKRHLEELKTDLVGNL
                     SHELKTPIAILRGYLETVSEHLNNPALCRELVDKALTNVDRQSSIVSDMVKLNMIETL
                     PELPMEEVNPARIIEGCVEILQPKAGDRNIALARSLADVDVTVRGNRFLAEEVFFNII
                     DNAINYNVPDGAVRILGLRDQAGLVVTVADTGIGIPAESLPRLFERFYRVDKSRSRAT
                     GGTGLGLSIVKHAAEIMGWRVEASSDGTGTTFTVRIA"
BASE COUNT          289 a          594 c          520 g          316 t
ORIGIN      
        1 cgaagagccc gcgcatcgtg aaggtcatcc ggctgggcat caacaacctc gctggcgtcc
       61 cctcggtggt cttcggcctc ttcggcctgt cgctgttcgt ggtgttcctc ggcttcggca
      121 cgtcgatcct cgcgggctcg ctcacgctgg gcgtactcaa tctcccggtg atcatccgct
      181 ccaccgagga agcgctcctg gtggtgccgg ggacctaccg cgaggcgtcg ctctccctgg
      241 gcgccacgcg ctggcaaacc atatacaaga tcattctccc gaacgccctc ccggggatcc
      301 tcacgggcgt catgctctcg ctgggccgcg cggccggcga gaccgcgccc atcatgttca
      361 cggcggcggc ctactacacg ccggagcttc ccacctcgat cttcaacgag atcatggcgc
      421 tcccctacca catctacgtg atggccacgg cggggacgca catccaggcg acacggcacc
      481 tccagtacgg cacggccatc gtgctgatcg cgctggtgct cgcgctcaac atgaccgggc
      541 tcatcctgcg ctaccgcttc cgcaagcgcc tcaaggggta gttccgcgcg ccgttcccgt
      601 cgaacatcga acggccccgc gcgtttaggc atatcacata acgccttccg ttcagccgcc
      661 gccctgatta ccggatgttc atacgctctt cgccgcgatc acccgccacc ggccggatgt
      721 cagcaatcga aaacccgatg gacgacgatg aacggggaga tacgcgcgcg aacgcctacg
      781 cgatcctgac ggtgaacgtg gtgcccgtcc cgtccgagct cgcctcgacg cgccagccca
      841 tgatctccgc ggcgtgcttc acgatggaca gcccgagccc cgtcccgccc gttgcgcgcg
      901 atcggctttt gtccacgcgg tagaagcgct cgaagaggcg cggcagcgac tcggcgggga
      961 tgccgatccc ggtgtcggcg acggtgacga cgagccccgc ttggtcgcgg agccccagga
     1021 tgcgcaccgc cccgtccggg acattgtagt tgatcgcatt gtcgatgata ttaaagaaga
     1081 cctcctcggc caggaagcgg tttccccgca ccgtgacgtc gacgtcggcg aggctgcgcg
     1141 ccagggcgat gttccggtcg cccgccttgg gctggaggat ctcgacgcaa ccctcgatga
     1201 ttcgcgccgg attgacctcc tccatgggaa gctcgggcag cgtctcgatc atgttgagct
     1261 tgaccatgtc gctcacgatg gacgattgcc ggtccacgtt cgtgagcgcc ttgtccacga
     1321 gctcgcggca caacgcggga ttattcaggt gttcgctcac cgtctccagg taaccccgca
     1381 ggatggcgat gggggtctta agctcgtgcg agaggttgcc cacgaggtcg gtcttgagct
     1441 cctcgaggtg ccgcttttcc gtgatgtcgt gcaacacgat gaggattccc tgcaccgcct
     1501 tctcctcggt gatgggcgaa atgaacacct cgcaatagcg gccgctcgca agcgcctcta
     1561 tgaaacgcgc ctccacgccc cgcgcaaggg agtactcgat cttcgcgtta aacgagcttc
     1621 cccgcaccac ctcgtaatac gcgcggccga acaccgcggc gcggatatcc aggagggaga
     1681 ggaaggcgcg attggcgatg agcacccgct tcgaatggt
//
LOCUS       DAORHF010000450         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_93140_length_1708_cov_5.557774, whole genome shotgun sequence.
ACCESSION   DAORHF010000450 DAORHF010000000
VERSION     DAORHF010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93140_length_1708_cov_5.557774"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PKJ16_19655"
     CDS             <1..228
                     /locus_tag="PKJ16_19655"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HNV49264.1"
                     /translation="MDGSVQSVIVDMKGVNAVDSTGLAALYVGKRKMEALKGTFALAN
                     VGKGIADLLRIAQMDFRIIEFNPGPLADEEE"
     gene            259..1413
                     /locus_tag="PKJ16_19660"
     CDS             259..1413
                     /locus_tag="PKJ16_19660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49265.1"
                     /translation="MGAIRGLMAAAVVAALCVFPGGPLLGARDDGKSVVVMASGSAPV
                     MGGDVKRARETAVQAALRAAVEEGVGMYITSRTRVENMAMIQDIIVKQSGGLAVIKRI
                     VREGAEKDVYRVQAEIAVSALSIIDVLRQNGLLRVWRVMVVIPEFHIQRPIVDPAAET
                     EFLRQFAAVGFKTMDQTAYRELRDKTPHYFKDNARAATFARGKGADILITGEAFSERS
                     MDATGSLMRGLVACNARVEVRAIAVDTGEVIYADAHTTPVPLLHTSESVAAKRALQSA
                     AEELAKKFVMVLVQRPAALTRPTVIEAGPFPGVQQATAFERDIRKLRGVKKVHREQFD
                     RGSLVLEAEVASDESDAFAAEIERRVSVPGHSLVVEGAARNVVRIRLMKK"
     gene            1457..>1708
                     /locus_tag="PKJ16_19665"
     CDS             1457..>1708
                     /locus_tag="PKJ16_19665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49266.1"
                     /translation="MKKLALIGIVLAGMAAFAGCRGGVDVVISRHGNPEEIRKVAIFN
                     LKLQNRGSYSTGDRVSDLLMHEFLKLGYDVVERTEIERLV"
BASE COUNT          318 a          497 c          606 g          287 t
ORIGIN      
        1 atggacggca gcgtgcagag cgtgatcgtg gacatgaagg gcgtcaatgc cgtcgattcc
       61 acggggctgg cggcgctgta tgtgggaaag cggaagatgg aggcgctcaa ggggacgttc
      121 gccctggcca acgtgggcaa gggcatcgcg gacctcctgc gaatcgcaca gatggacttt
      181 cgaatcatcg aattcaaccc cggccccctg gccgacgagg aggagtaagc gtcgccgcga
      241 tggcgacgcg catgcgatat gggagcgata cgtgggctga tggccgcggc cgttgttgcg
      301 gcgctgtgcg tgtttccggg ggggccgctc ctcggtgcgc gtgatgacgg aaagagcgtg
      361 gtggtcatgg ccagcggaag cgcgcccgtc atggggggcg acgtgaagcg ggcgcgtgag
      421 acggccgtcc aggccgcatt gcgcgccgcg gtggaggagg gcgtgggaat gtacatcacc
      481 tcgcgcacgc gcgtcgagaa catggcgatg atccaggaca tcatcgtcaa gcagtcgggg
      541 gggctggccg tcatcaagcg gatcgtgcgc gagggcgcgg agaaggacgt ctatcgcgtc
      601 caggccgaga tcgcggtgag cgcgctgtcg atcatcgacg tgttgcggca gaacggcctt
      661 ctgcgcgtct ggcgcgtcat ggtggtgatc cccgagttcc acattcagcg tcccatcgtc
      721 gatcccgcgg ccgaaacgga gtttctccgc cagttcgccg cggtcgggtt taaaacgatg
      781 gaccagacgg cttaccggga actgcgcgac aagaccccgc actacttcaa ggacaatgcg
      841 cgcgcggcga ccttcgcgcg cggcaagggc gccgacatcc tgattacggg cgaggccttc
      901 agcgagcgct cgatggacgc gaccgggtcg ctcatgcgcg ggctagtcgc ctgcaatgcc
      961 cgcgttgagg tgcgggcgat cgccgtggac accggcgagg tgatatacgc cgacgcccat
     1021 acgacgccgg tgccgcttct gcacacctcc gaatccgtcg ccgcgaagcg ggcgttgcag
     1081 agcgcggcgg aggagcttgc gaaaaagttc gtgatggtgc tcgttcagcg gcccgccgcg
     1141 ctgacgcgcc cgacggtgat cgaggccggg ccctttcccg gcgtgcagca ggccacggcc
     1201 ttcgagcgag atatcaggaa gctccggggc gtgaaaaaag tgcaccgcga gcagttcgac
     1261 cggggcagcc tggtcctcga ggccgaggtc gcctccgacg agagcgacgc attcgccgcc
     1321 gagatcgaac ggcgggtgag cgtccccggg cattcgctcg tggtggaggg ggccgcgcga
     1381 aacgtcgtgc ggatcaggct gatgaaaaaa tgaattgtcc ttgcataaaa taccgtttca
     1441 cgatttcaat ccacccatga aaaaattagc gcttatagga atcgtccttg ccggcatggc
     1501 cgcattcgcg ggctgccggg gcggcgtgga cgtggtcatt tcgcgccacg gcaacccgga
     1561 ggagatacgc aaggtcgcca tcttcaattt aaaactccag aaccgcggat cgtactcgac
     1621 cggtgaccgg gtgtccgatt tgctcatgca tgagttcctt aaactcgggt acgacgtggt
     1681 ggagcgcacc gagatcgaac gactggtg
//
LOCUS       DAORHF010000451         1682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_95601_length_1682_cov_4.610326, whole genome shotgun sequence.
ACCESSION   DAORHF010000451 DAORHF010000000
VERSION     DAORHF010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95601_length_1682_cov_4.610326"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PKJ16_19670"
     CDS             complement(<1..424)
                     /locus_tag="PKJ16_19670"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HNV49267.1"
                     /translation="MIEEGKYAVIHYTGTFTNGEVFDSSVDGDPFEFLVGSGQVIPGF
                     DAAVRDMKIDEEKDVFISADDGYGQYDESLVYHFPIDQVQGQFTPEVGMTIGLQLEDG
                     RQMPATITEIGADAITVDMNHPLAGKDLSVSYKMGDPDF"
     gene            complement(587..>1682)
                     /locus_tag="PKJ16_19675"
     CDS             complement(587..>1682)
                     /locus_tag="PKJ16_19675"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HNV49268.1"
                     /translation="VTDLSFFEANGVITRALDSAADTPNRVHHFLPKCGRVYLMKGGE
                     AGDDLRAISAENRRRYATVMDRTYELPNTDHQRRLIVFEKIDSARTATYRILRDENES
                     VGTVVTSPDNKSFKEMKRLTAIEGIRKQRRLIVSGPRQVVEFSRSFPELTQGVVIGDG
                     LVETDDEIMRLVRAHAESRTLWLLKKSLFNELDGFNTGRPLLVAALPDIPAWDGTLAP
                     GCSLVLPFQDPVNAGSAIRSAAAFGVARIIVLKECAHSFHPRAVRSSGGAVFHAPLAA
                     GPSLAEFAAIVRESRLPVVSLDAAGTPIDEFSFPVSFLLVPGMEGPGLPDDLKRDAVS
                     IPIARDVESLNAAVAASIALFAWKTRQGKA"
BASE COUNT          305 a          502 c          548 g          327 t
ORIGIN      
        1 agaagtccgg atcgcccatc ttgtagctga cgctgaggtc cttgcccgcg agcggatggt
       61 tcatgtccac ggtgatggca tcggcgccga tctcggtgat ggtcgcgggc atctgccgcc
      121 cgtcctcgag ctggaggccg atggtcatcc ccacctcggg agtgaattgc ccctgtacct
      181 ggtcgatcgg gaaatggtag accagcgatt catcgtactg gccatagccg tcgtcggctg
      241 agatgaagac gtccttctcc tcgtcgattt tcatgtcgcg caccgcggcg tcgaagccgg
      301 ggatcacctg ccccgatccg acgaggaatt cgaagggatc gccatcgacg ctcgaatcga
      361 acacctcgcc gttcgtgaag gttcccgtat agtgtatgac cgcgtatttt ccttcttcaa
      421 tcattggtcg ctccttggaa gcagttttca accgaatcaa tgtgcgtatt ggagagaagc
      481 gtgggaatgg gtatctcgaa taggtacatt gccgggtact gtagcgcgct ttcggttggt
      541 gtcaataaaa tatttcatgc cgtaccgggg aaaagccaac cgcagtctac gctttcccct
      601 gccgcgtttt ccacgcaaac agcgcgatcg aggccgcgac cgccgcattg agcgattcga
      661 cgtcgcgtgc gatggggatt gatacggcgt cgcgcttgag atcgtccgga agacccggtc
      721 cctccatgcc gggcaccaga aggaaggaca ccggaaacga aaattcgtcg atgggcgtcc
      781 cggccgcatc cagcgatacg accggaaggc gcgattccct gacgatggcc gcgaactctg
      841 cgagcgacgg tccggcggca agcggcgcgt gaaacaccgc accgcccgag gagcgcaccg
      901 cgcgcggatg aaaggagtgc gcgcattcct tcaggacgat gatgcgcgct accccgaacg
      961 ccgccgctga gcggatcgcc gatcccgcat tgaccgggtc ctggaacggg aggacgaggg
     1021 agcagccggg cgcgagcgtg ccgtcccatg cggggatgtc gggcagcgcc gcaacgagaa
     1081 gcggccggcc ggtgttgaag ccgtccagtt cgttgaagag ggatttcttc agaagccaga
     1141 gcgttctcga ttcggcgtgg gcccggacca gccgcatgat ctcgtcatcg gtctcgacga
     1201 gcccgtcgcc gatgacgacg ccctgcgtca gttccggaaa ggatcgcgag aactcgacaa
     1261 cctggcgcgg tccggagacg atcaggcggc gctgtttccg gatgccctcg atggcggtga
     1321 gccgtttcat ctccttgaac gacttgttgt cgggcgaggt gacgacggtg ccgacgctct
     1381 cgttctcgtc gcgcaggatg cggtaggtcg ccgttcgcgc gctgtcgatc ttttcgaaca
     1441 cgatcaggcg gcgctgatgg tccgtgttcg ggagttcgta tgtgcggtcc atgacggtcg
     1501 cgtaccgccg gcggttttcc gcggatatcg cgcgcaggtc gtccccagcc tctccgccct
     1561 tcatgaggta cacgcgcccg catttcggga ggaagtgatg gacgcggttg ggggtgtcag
     1621 cagcggagtc cagcgcgcgg gtaatcacgc cgttcgcctc gaaaaacgag aggtccgtca
     1681 cc
//
LOCUS       DAORHF010000452         1675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_96248_length_1675_cov_4.671605, whole genome shotgun sequence.
ACCESSION   DAORHF010000452 DAORHF010000000
VERSION     DAORHF010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96248_length_1675_cov_4.671605"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="PKJ16_19680"
     CDS             complement(<1..815)
                     /locus_tag="PKJ16_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HNV49269.1"
                     /translation="MSVEIKKSYEEINAKIRQGTAVVVTADEMVGIVKRDGAKSAFNT
                     VDVVTTGTFGTMCSSGAFLNFGHTKPRMKASKVWLNEVEAYGGIAAVDCYIGATQVQD
                     DDPLNKVYPGSFHYGGGHVIEDLIAGRTVKLRAKGYGTDCYPLKEYETAITIDALRDA
                     ILYNPRNAYQNYNCAVNLSRRTIYTYMGILRPNMGNATYSSAGELSPLLKDPFYWTLG
                     TGTKIFLGGAVGAIAWRGTQHAPDCLRGDNSVPREGAGTIAVTAVCSAFLFLML"
     gene            1130..1579
                     /locus_tag="PKJ16_19685"
     CDS             1130..1579
                     /locus_tag="PKJ16_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49270.1"
                     /translation="MTTARLPMDLKTSGKVSLVLFIILLIEGLFVNLFVGSEAANDAP
                     MERCISAVRYAMPALSAFGIAMALGLVMGRPWARRGMIAVCAGWITFIGVMGLAMGRA
                     VLSARPDRFAPGTGRDEALVVLLFAVGAFTFLIALAIRHFLRAGRAS"
BASE COUNT          260 a          514 c          541 g          360 t
ORIGIN      
        1 agcatgagga acagaaacgc cgagcagacc gcggtgacgg cgatggtccc cgcgccctcg
       61 cgtgggacgc tgttgtcccc gcgcaggcag tccggcgcgt gctgggtgcc ccgccaggcg
      121 atcgcgccca cggcgccgcc gaggaagatc ttggtcccgg tgcccagcgt ccagtagaac
      181 gggtccttga gcagcggcga gagctccccg gcgctcgagt aggtggcgtt tcccatgttg
      241 ggacggagga ttcccatgta ggtgtagatg gtccgccggg agaggtttac ggcgcagttg
      301 tagttctgat aggcgttgcg cggattgtac agaatggcgt cgcgcagggc gtcgatggtg
      361 atggcggtct cgtactcctt gagcggatag cagtccgttc cgtagccctt ggcccgcagc
      421 ttcaccgttc gcccggcgat gaggtcctcg atcacgtgtc cgccgccata gtgaaacgag
      481 ccgggatata ccttgttgag cgggtcgtcg tcctgcacct gggtcgcgcc gatgtagcag
      541 tcgaccgccg cgatgccgcc gtacgcctcc acttcgttga gccacacctt cgaggccttc
      601 atgcgcggct tggtgtgccc gaagttgaga aaggcgccgg aggagcacat ggtcccgaac
      661 gtgccggtgg tgaccacgtc caccgtgttg aatgcgctct tcgcgccgtc gcgcttcacg
      721 atccccacca tctcgtcggc ggtgacaacg acggccgtgc cctgcctgat cttcgcgttt
      781 atctcttcgt agcttttctt gatctcgacg ctcatggcgc gctccctaat ctctgctgcg
      841 tccctccgca cacggcgtac ggaagtgtga tgatgaagat gtatcggcgg tattccgtca
      901 atttgatttt atagtatttg caaaaaacag cgtcgcggca tagcgtggcc gcgacggtct
      961 cacggatcgt acgcggcgcg tgaagggagg tgacatgttt ccgggaatgc ccgaactgat
     1021 tcttattgtc gtcatcatcg gcggctgtat tgccgtcgcg gttatcatga cgcgtcgtga
     1081 cacgcgcgcc aagaaataat cccgcctgga ggatgaggcc ctgcccgcca tgacaaccgc
     1141 acgccttccg atggatctga aaacctccgg aaaggtctct ctggtcctct tcatcatttt
     1201 gttgatcgag ggcctgttcg tgaacctgtt tgtcggcagt gaggcggcga atgatgcgcc
     1261 catggaacga tgtatttccg ccgttcgcta cgccatgccc gcgctctcgg cattcgggat
     1321 tgcgatggcc ctcggtcttg tcatggggcg tccgtgggcg cgtcggggca tgatcgccgt
     1381 gtgtgccggc tggatcacgt tcatcggcgt catggggctc gcgatgggcc gggccgtcct
     1441 atcggcgcgg ccggaccggt tcgcgccggg aacgggccgg gacgaggcgc tcgtcgtgct
     1501 cctgttcgcc gtgggcgcgt tcacgttcct catcgcgctc gccatccgcc attttctgag
     1561 ggccggccgc gcatcgtgat cgatgaaacc accctgacat cgcgcataac gcagcagtac
     1621 gccgggcgat cgctcatcga tttcctgagc ggccggttcc gctaccgcgg tcgcg
//
LOCUS       DAORHF010000453         1647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_98998_length_1647_cov_4.717965, whole genome shotgun sequence.
ACCESSION   DAORHF010000453 DAORHF010000000
VERSION     DAORHF010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98998_length_1647_cov_4.717965"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="PKJ16_19690"
     CDS             <1..313
                     /locus_tag="PKJ16_19690"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HNV49271.1"
                     /translation="TVRSGAPRWPFTSSFVKNLVLERPVPDARLCTRCHQCRDICPAR
                     AISTTAKNEKTPRFDYALCIRCYCCMEICPEGAIELQKGIVKRIIMRRSVGKNTIEPM
                     R"
     gene            330..872
                     /locus_tag="PKJ16_19695"
     CDS             330..872
                     /locus_tag="PKJ16_19695"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HNV49272.1"
                     /translation="MKKAIGIGIVVVAGSFIFLVYNGVFNDMKVSEAELGPFTFVFER
                     HRGPYHATGPLFAKVSATLAGDLACAQLYGIGVYYDDPRTVAPEALRSDIGYAIEKPD
                     ADTAIKIAARLAVRDIPRTRYAVIAFPFKNRLSVMIGAMKAYDPLYGYVSRQGYRIAP
                     AMELYDRKGGTILYAVPLAR"
     gene            complement(915..>1647)
                     /locus_tag="PKJ16_19700"
     CDS             complement(915..>1647)
                     /locus_tag="PKJ16_19700"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008395678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HNV49273.1"
                     /translation="FDYAQKHDIPLPITRDKPYSMDRNILHISYEGGILEDPFNPPNE
                     DMFILTKSPEKAPDKATIIDIDFEKGVPVAIDGERLGPVALMKKLNTIAGENGVGRID
                     IVENRLVGIKSRGVYETPAGTVLQIAHRDLESITLDRDTQHLKDRIAVEYAILVYNGL
                     WFTRRREALDAFIDTTQAHVTGTVRVKLYKGNCMVIGRKSPVTLYDPDIATFDASELY
                     DQRDASGFLNIFGLPSKVEALLRKK"
BASE COUNT          316 a          474 c          501 g          356 t
ORIGIN      
        1 caccgtgcgt tccggcgcgc cacgctggcc gttcacgtcg agtttcgtca agaacctcgt
       61 tctcgaacgg ccggtcccgg atgcgcggct gtgcactcgg tgtcatcaat gccgcgacat
      121 ctgtccggca cgcgccatat cgacgaccgc gaagaacgag aaaacacccc ggttcgacta
      181 tgcgctctgc atccggtgct actgctgcat ggagatttgt cccgagggcg ccatcgagct
      241 gcaaaagggg atagtcaagc gcatcatcat gcgtcgctca gtcggtaaga acacaatcga
      301 accaatgcgc taaccgcagg agatcgagta tgaaaaaggc tatcggaatc ggtatcgtag
      361 ttgtggccgg atcttttatc tttcttgtct acaacggcgt attcaatgac atgaaggtgt
      421 ctgaggcgga actcggtccg ttcacgtttg tctttgaacg tcaccggggc ccgtatcatg
      481 caaccggccc gctgttcgcg aaggtgtccg caacccttgc cggtgatctt gcctgcgcgc
      541 agctgtacgg gatcggggtg tattatgacg atccgcgcac ggttgcgcca gaagcgcttc
      601 ggagcgacat cggatatgcg atcgagaaac ccgacgcgga tacggccata aaaatcgccg
      661 cgcgactcgc tgtgcgtgac atcccgcgaa cgcggtatgc ggtgattgcg ttcccattca
      721 agaaccggct gtcggtcatg atcggcgcga tgaaggccta tgatccgctc tatggatatg
      781 tttcgcgcca aggataccgc attgcgccgg cgatggagct atacgacagg aagggcggga
      841 caatactcta cgccgtgccg ctggcgcgat agaaaacgtt ggcgacggga gcatcccgcc
      901 gccaacggca acgattactt tttcctgagc aacgcttcga ctttcgaggg aagaccgaag
      961 atgttcaaaa atcccgacgc gtcgcgttgg tcgtagagct cgctcgcgtc gaaggtcgcg
     1021 atatcggggt cgtagagcgt caccggcgac ttccgcccaa tcaccatgca attccccttg
     1081 tacagcttca cgcggaccgt tccggtcacg tgcgcctgtg tggtgtcgat gaaggcgtcg
     1141 agcgcctcgc ggcggcgcgt gaaccacagt ccattgtaca ccaggatggc gtattcaacc
     1201 gcgatgcggt ctttcagatg ctgggtatcg cggtccaggg tgatcgattc gaggtcgcga
     1261 tgcgcgatct ggaggacggt gccggccggc gtttcataca cgccgcgcga tttgatcccc
     1321 accagccggt tctcgacgat gtcgatgcgc cccacgccgt tttcgccggc gatcgtgttc
     1381 agcttcttca tcagtgcgac cggcccgagc cgctctccgt cgatcgcgac gggcacgccc
     1441 ttctcgaaat cgatgtcgat gatcgtcgcc ttgtccggtg ccttctcggg gcttttcgtg
     1501 agaatgaaca tgtcttcatt gggcggattg aaggggtcct cgagaatgcc gccctcgtag
     1561 gagatatgca ggatattgcg gtccatgctg tagggcttgt cgcgggtgat gggcagcggg
     1621 atgtcgtgct tctgcgcata gtcgaac
//
LOCUS       DAORHF010000454         1643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_99399_length_1643_cov_4.056045, whole genome shotgun sequence.
ACCESSION   DAORHF010000454 DAORHF010000000
VERSION     DAORHF010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99399_length_1643_cov_4.056045"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PKJ16_19705"
     CDS             complement(<1..418)
                     /locus_tag="PKJ16_19705"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HNV49274.1"
                     /translation="MNIIDATIQVNPYSRPGRIRPETKAVILHWVANPGTSARANRDY
                     FASLAKQTTAPFRYASAQYIVGLSGEILRVMPEEEVAWHCGSSQVDPASGRIYTDWAR
                     EVIGEKYASRWSTPNHATVGIELCHPDSSGVFTAETE"
     gene            complement(415..744)
                     /locus_tag="PKJ16_19710"
     CDS             complement(415..744)
                     /locus_tag="PKJ16_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49275.1"
                     /translation="MPIETITALALGALVPIVVGGIVTAIYRAVRRRVLVQSPESRQL
                     EQLVPLVNALARTTGPQNDALIAILEAQKGVRNGNVDRALDRMRETRDEFNQFLVSSA
                     LVDKEAS"
     gene            complement(757..>1643)
                     /locus_tag="PKJ16_19715"
     CDS             complement(757..>1643)
                     /locus_tag="PKJ16_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49276.1"
                     /translation="ASLPATANEDDTALAYSATAEERGIYAMIAGTWTKLAAPSVDAL
                     SRAWPDISRATLTEASGGFAGGPYGVADDYFGSGVDVFSVLAAQTAFIESLFAKQITI
                     QTGGLIKSSNYSAGTAGFQVEADGSAEFNDVTVRGAFEGTVDATSIEAVVETGTTTVK
                     KIASIAGTSSLLESGYLEFAGAGEITISYSYSIYIRDGGIVDTVQRMRPRLNETSYTT
                     IRRKTVGASVETGDDSFDVVVGEGDIFKLSGRYAMAAVLLEQCQYTNFSIKVNGPAGI
                     LSWLGYTGKSGASYVAPR"
BASE COUNT          303 a          502 c          496 g          342 t
ORIGIN      
        1 cttctgtctc ggcggtgaaa acgccgcttg agtcgggatg gcagagctcg atccctaccg
       61 ttgcgtggtt cggggtcgac catcgcgagg cgtacttctc gccgatcact tcgcgcgccc
      121 aatcggtgta gatccgtccg gaggctggat ccacttggct cgagccgcag tgccaggcga
      181 cttcttcctc gggcatcacg cgcaggatct cgccggagag accgacgatg tactgggcgg
      241 acgcgtagcg gaacggggcc gtcgtctgct tcgcgagcga ggcgaagtaa tcccggttcg
      301 cgcgcgcgga ggttccaggg ttcgcgaccc aatgaaggat gaccgccttc gtctcggggc
      361 ggatccgccc cggtcgtgaa tacgggttga cctggatcgt cgcgtcgatg atgttcatga
      421 cgcctcctta tccacgagcg cggaggagac aaggaactgg ttgaattcat cgcgcgtctc
      481 gcgcatccgg tcgagcgcac gatcgacgtt gccgttgcgc acgcctttct gcgcttcgag
      541 gatcgcaatg agcgcgtcgt tctgcgggcc ggtcgttcgg gcgagcgcgt tgacgagcgg
      601 gacgagctgt tcgagctggc gcgattccgg cgactggacg agcacgcgtc ggcggacggc
      661 gcggtagatc gccgtgacga tcccgccgac gacaatcggg acgagtgctc cgagcgcgag
      721 ggcggtgatg gtttcgatcg gcataaggtc tcctgtttac cttggcgcga cgtagctagc
      781 gccggacttt cctgtgtatc caagccacga caagatccct gcaggtccgt tcacctttat
      841 cgagaaattt gtatattggc actgttccaa caggacggcg gccatcgcgt atcgaccgga
      901 cagcttgaaa atatcgcctt ctcctaccac gacatcgaat gagtcatcac cagtttctac
      961 tgacgctcca actgtttttc tccgtatcgt tgtgtagctg gtctcattaa gcctagggcg
     1021 catgcgctgc acggtgtcga caatcccgcc gtcgcgtata tatatgctgt acgaatagga
     1081 aatcgttatt tctccggcac cggcaaattc aagatacccg gactcaagca gcgatgaagt
     1141 accggcgatg cttgctattt tttttaccgt ggtcgttccg gtctcgacga cggcctcgat
     1201 actcgttgca tccaccgttc cctcaaacgc tccgcgcacc gtgacgtcat tgaactcagc
     1261 cgaaccatcg gcctcaacct ggaagccggc agtccctgcg gaataattcg aggactttat
     1321 cagccccccg gtttgaatcg tgatctgctt cgcaaagagc gattcgatga acgcagtctg
     1381 tgccgcgagc acggagaaca cgtccacgcc cgacccgaaa taatcatcgg ccacgccata
     1441 cggcccgccc gcgaatccgc ccgatgcttc cgtgagcgta gccctcgaaa tgtccggcca
     1501 tgcgcgcgag agagcgtcca ccgaaggggc ggcgagcttc gtccacgttc cggcgatcat
     1561 cgcgtagata ccgcgctcct ccgcggtagc cgagtacgcg agagccgtgt cgtcctcgtt
     1621 tgcggtcgca gggagcgagg cga
//
LOCUS       DAORHF010000455         1636 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_100118_length_1636_cov_4.362429, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000455 DAORHF010000000
VERSION     DAORHF010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100118_length_1636_cov_4.362429"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..994)
                     /locus_tag="PKJ16_19720"
     CDS             complement(41..994)
                     /locus_tag="PKJ16_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HNV49277.1"
                     /translation="MIIQLRGVRGSLPTPMGNREYRAKLASILSRAVEAGIKSAAEIG
                     AFIDALPEDLGYNYGGDTTCVTVTSRSGRTYVLDCGTGIRPLGDEMVSGPWGVGRGAV
                     DIFITHTHWDHIQGLPFFKPLYIPGNDFTFYSPYPDLADRFARQMEFTFFPAPFDKTG
                     STKRFVHMNPEEPLELEEGLTVDCYPLKHPGGSVAYRFREGDKIFIFCTDCEFTGETM
                     ESFGTHSAFFLDADLLLLDSQYTLDESFNKFDWGHTSYTVAVNCAIRWRAKRLVLTHH
                     EPSYSDAKLREIHLDALNHRNEMRCDSPEILMAREGMVFQL"
     gene            1211..>1636
                     /locus_tag="PKJ16_19725"
     CDS             1211..>1636
                     /locus_tag="PKJ16_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49278.1"
                     /translation="MKNLIPIVLSAVLLASCSTFKGKLTVQQTLSEKDKIVAAANPAM
                     RYLLTEDLKKKRVVITDATVSDVIESLHIDYDFCVVVTMTVDANKVDCYLYSDDLKTI
                     SKLVKGSTRIDAVGDFEKFFTLLDEKYTKIEVLDACIDIK"
BASE COUNT          366 a          477 c          491 g          302 t
ORIGIN      
        1 ccgacatcat cttgtactac cgctacgcgg cgcggccgga tcagagctga aagaccatcc
       61 cttcgcgagc catgaggatc tcaggggaat cgcaccgcat ctcgttgcga tggttgagcg
      121 catcgaggtg tatctcgcgg agcttggcgt cggaatagga tggctcgtgg tgagtgagga
      181 cgagccgctt cgcgcgccat cggatcgcgc agttcacggc caccgtatac gaggtgtggc
      241 cccaatcgaa cttgttgaac gactcgtcca gcgtgtactg ggaatccagc aggagcaggt
      301 cggcgtcgag gaagaaggcg ctgtgcgtcc cgaacgactc catggtctca cccgtgaact
      361 cgcaatccgt gcaaaagatg aaaattttat cgccttcgcg aaatcggtac gcgacgctcc
      421 cgcccgggtg cttgagggga taacagtcga ccgtaaggcc ctcctccagc tcaagcggct
      481 cctccgggtt catgtgcacg aaccgcttgg tcgagccggt tttatcgaac ggggccggaa
      541 aaaaggtgaa ctccatctgg cgggcgaaac ggtccgcgag atccgggtac ggcgagtaaa
      601 aggtgaaatc attgccggga atgtagagcg gcttaaaaaa cggaaggccc tgtatgtggt
      661 cccagtgggt gtgggtgatg aaaatatcaa ccgcaccccg ccccacgccc cacgggccgg
      721 aaaccatctc gtcgcccaga ggacggatgc cggtgccgca atcaaggaca taggtccgcc
      781 cgctgcgcga ggtcacggtc acgcaggtgg tgtcgccccc gtagttgtag cccaggtcct
      841 cgggcagggc atcgatgaac gcgccgatct cggccgcgct tttgataccc gcctcgacgg
      901 cgcgggaaag tatcgacgca agtttcgcgc ggtattcgcg gtttcccatc ggcgtgggga
      961 gcgatccgcg cactccccgg agctgaataa tcatgcgggc accttgaaga atgatctcac
     1021 tgccgaacgg cacgcactgc gccggcgtcg gagcgccggt cccccggtac gatgcttgta
     1081 catcgcactt gaagcgggat tgtcaagata aaaaatggcg catcgctact atcggccgcg
     1141 aattaatgta ttgacaggac cggcgccgcc aaccccaaat gcagataatt cagatacgcg
     1201 gagtggcccc atgaagaacc tcatcccgat agtcttgagc gccgtcctgc tggcctcctg
     1261 ctcgacgttc aagggcaaac tgaccgttca acaaaccctg agcgaaaagg acaagatcgt
     1321 tgcggccgca aatcccgcga tgcgctattt gctgaccgaa gacctcaaga agaagcgcgt
     1381 cgtgatcacc gacgcgacgg tgagcgacgt aatcgagtcg ctgcacatcg attacgactt
     1441 ctgcgtggtg gtgacgatga cggtggacgc gaacaaggtc gactgttatc tgtattccga
     1501 cgacctcaag accatatcca agctcgtaaa ggggtccacc cggatagacg cggtgggcga
     1561 cttcgagaag ttcttcaccc tcctcgatga gaagtacacc aagatcgagg tcctcgacgc
     1621 ctgcatcgac atcaag
//
LOCUS       DAORHF010000456         1623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_101446_length_1623_cov_4.513393, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000456 DAORHF010000000
VERSION     DAORHF010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101446_length_1623_cov_4.513393"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..>1623
                     /gene="fdhF"
                     /locus_tag="PKJ16_19730"
     CDS             68..>1623
                     /gene="fdhF"
                     /locus_tag="PKJ16_19730"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:311..313,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HNV49279.1"
                     /translation="MQGDKRITKPLIRKNGKLEESSWEEALGLVASKLSDIKAKSGPD
                     SIMAFSSAKCTNEENYLMQKFMRAVIGTNNVDHCARLUHSSTVAGLAATLGSGAMTNS
                     IREIPLTEAFLVIGSNTTDNHPVIGSMIKNEVINKGKKLVVVDPRRIDLAKYADVFLQ
                     ILPGTNIAILNGLMHVILKENLQDKDYIANRCEGFDDVAKTLEKYTPEYVAGICGLKN
                     PDDIVKAARILAKATPMAAYYCMGITQFKSGVNGVKSVANLQMMLGNIGVAGGGVNPL
                     RGQSNVQGACDMGALNAVYPAYQPIASEDNKKKFMEAWKVAFDLSMKPGLTIVESLNG
                     AIAGDVKALYVLGENPVMSDPNQHHVIEALEKLDFLVVQDIVLTETAQYAHVVLPGCA
                     FLEKEGTVSNTERRVQLMHKVVDPIGDVRDDWWIITQIANRMGASWSYAKAEEIFEEV
                     RKVTPSYAGITYARLEKELIQWPCPTEEHPGTQFLHKDRFSRGLGLFTAIDYTPPAEE
                     VDPEYPLVLTT"
BASE COUNT          346 a          508 c          493 g          276 t
ORIGIN      
        1 cctgggcaac gacgacggcc ccaatcgcgg ccatctctgc gtgaagggcc gcttcggctt
       61 cgatttcgtg cagggggaca agcgcatcac caagcccctc atccggaaga atggaaaact
      121 cgaggaatcg tcctgggagg aggcgctcgg tctggtcgcc tcgaagctct ccgacatcaa
      181 ggcgaagagc ggccccgact ccatcatggc cttctcgtcg gccaagtgca ccaacgagga
      241 gaactacctg atgcagaaat tcatgcgggc ggtgatcggc accaataatg tcgatcactg
      301 cgctcgacta tgacacagct ccacggtcgc cggtctggcg gccacgctcg gttctggtgc
      361 catgacgaat tcgatacgtg agatacccct taccgaggcc tttctggtca tcggctcgaa
      421 cacgaccgac aaccatccgg tcatcgggtc catgatcaag aacgaggtca tcaacaaggg
      481 taaaaaactg gtggtcgtcg atccgcggcg catcgatctg gcgaaatacg ccgatgtgtt
      541 cctccagatc cttccgggga ccaatatcgc catcctgaac ggcctgatgc acgtgatttt
      601 aaaggaaaat cttcaggaca aggactacat cgccaatcgc tgcgagggct tcgacgatgt
      661 cgcgaaaacg ctcgagaagt atacgcccga gtacgtcgcc ggcatctgcg gcctcaagaa
      721 ccccgacgac atcgtgaagg ccgcgcggat cctcgcgaag gccacgccca tggcggcgta
      781 ctactgcatg ggcatcacgc agttcaagtc gggcgtcaac ggcgtgaaga gcgtcgcgaa
      841 cctgcagatg atgctgggca atatcggcgt cgcgggcggc ggcgtgaacc cgctgcgggg
      901 tcagagcaat gtgcagggcg cctgcgacat gggcgcgctc aatgccgtct acccggccta
      961 tcagcccatc gcgagcgaag ataataagaa gaaattcatg gaggcctgga aggtcgcctt
     1021 tgacctgtcg atgaagcccg gcctcaccat cgtggagtcg ctcaacggcg ccatcgccgg
     1081 cgacgtgaag gcgctctacg tgctcgggga aaaccccgtg atgtccgatc ccaaccagca
     1141 ccacgtgatc gaggcgctgg agaagctcga cttcctggtg gtgcaggaca tcgtcctcac
     1201 cgagacggcg cagtatgcgc acgtggtgct gcccggctgc gccttcctcg agaaggaggg
     1261 gacggtctcc aacaccgagc gccgcgtcca gctcatgcac aaggtcgtcg atcccatcgg
     1321 cgacgttcgc gacgactggt ggatcatcac ccagatcgcc aaccgcatgg gcgcctcgtg
     1381 gagctatgcg aaggccgagg agatcttcga ggaggtgcgg aaggtcacgc cgtcgtacgc
     1441 cggcatcacc tacgcgcgtc tcgaaaagga gcttatccag tggccctgcc cgaccgagga
     1501 gcatcccggc acgcagttcc tgcacaagga ccgcttcagc cgcggcctgg gactcttcac
     1561 ggcgatcgac tacacgccgc cggcggagga ggtcgatccg gagtatccgc tcgtgctcac
     1621 cac
//
LOCUS       DAORHF010000457         1610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_102832_length_1610_cov_4.047588, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000457 DAORHF010000000
VERSION     DAORHF010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102832_length_1610_cov_4.047588"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="PKJ16_19735"
     CDS             <1..1221
                     /locus_tag="PKJ16_19735"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HNV49280.1"
                     /translation="DLPCAWADEDRVRQVLSNLVGNAIKFTNEGSIDLTALVVNRTDK
                     NGGPGPGAMIEVRVIDTGIGVPEDHRESIFDAYRQADGSDTRTYGGIGLGLAIAKRIV
                     EAHHGSIWMTPGERGGSVFTFTLPVVDAAPLNSPEGYGIKEASGDPRPDTNAPDALPE
                     AICDAAFDGSPVVLAVDDDPVNLTVLRGILEPRGCIVRTAANGISALDLIERDDSIRL
                     VLLDIMMPIMSGFEICRRIRANRSPGELPVIMLTAKNMMTDIDAAFAAGANDYIMKPF
                     QPAELLARAGTMLAFRKVRRAMAESLTVRDGGTAYSIRFDDIVFITAHLKNIVVHTVN
                     GEVELPIILKEIADRLPPDIFIRIHKSHIVNLRYIHSVSHVLSGRYKVRLLDENETEL
                     PVGAAYLESLRKKI"
     gene            1415..>1610
                     /locus_tag="PKJ16_19740"
     CDS             1415..>1610
                     /locus_tag="PKJ16_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49281.1"
                     /translation="MIRTTAHLMSLASKFYRMTAIVCLLALVAPACNRDVYDNLFDPV
                     NQYTVTFDSQGGTAVSAQTVD"
BASE COUNT          330 a          511 c          482 g          287 t
ORIGIN      
        1 gacctcccct gcgcctgggc cgacgaggac cgcgtccggc aggtgctctc gaacctcgtg
       61 ggcaatgcga tcaagttcac gaacgagggg agtatcgacc ttacggcgct cgtcgtcaat
      121 cggacggata aaaacggcgg gccgggccca ggcgccatga tcgaggtgcg cgttatcgac
      181 acggggatcg gcgttcccga ggaccatcgc gaatcgatat tcgatgccta ccggcaggcc
      241 gacggaagcg acacgcgcac ctacggaggc attggcctgg ggctggccat cgcaaagcgg
      301 attgtggagg cgcaccacgg ctcgatttgg atgacgcccg gagagcgcgg gggctcggtt
      361 ttcaccttta cgctgccggt tgtcgacgcc gcgccactca actcaccgga ggggtacggc
      421 atcaaagagg cgtccggcga tccgcgcccg gacacgaacg cgccggacgc gcttccggag
      481 gctatatgcg atgccgcgtt tgacggctcc ccggtcgtcc ttgcggtcga cgacgacccg
      541 gtgaacctga cggtcctccg gggcatcctc gagccgcggg gatgcatcgt gaggaccgcc
      601 gccaacggga tcagcgcgct tgacctgatc gaacgcgacg actccattcg gctggtgctc
      661 ctggacatca tgatgcccat catgtcggga ttcgagatat gccggcggat acgcgcgaat
      721 cgatcgccgg gcgagcttcc ggtgatcatg ctcacggcca agaacatgat gaccgacatc
      781 gacgcggcct tcgcggccgg ggccaacgac tacatcatga agccctttca gccggccgag
      841 ctcctcgcgc gcgcgggcac catgctcgca ttcaggaagg tccggcgggc gatggccgag
      901 agcctcaccg tccgcgacgg tggaacggcc tacagtatcc ggttcgacga tatcgtcttc
      961 atcaccgccc atctgaaaaa catcgtcgtc cacaccgtaa acggcgaggt cgagctgccg
     1021 atcatattga aggagatcgc cgaccggctc ccgccggaca tattcattcg catccacaag
     1081 agccatatcg tcaacctgcg gtacatccac agcgtctccc atgtgctgtc gggccgttac
     1141 aaggtgcgtc tcctcgacga aaacgagacg gagctccccg tgggggcggc ctacctggaa
     1201 tcactgcgta agaaaattta attgtcgcgc accgttcatt cccagcgagg cacggatcat
     1261 tcccagacca ttgacatatc tgccgtcagt gtttatgtta ccgtaaagca aagtccgcaa
     1321 acgcacactg cgatcatacg actgcgtcgc gggcaggttc gccatgtaca gatgcgatac
     1381 cattaccgca gatgcgcgtg agaagattgc acgaatgata cgaacaaccg cacatctgat
     1441 gagcctcgca tcaaagtttt accgcatgac cgcgatcgtc tgccttctcg ccctcgtcgc
     1501 cccggcctgt aaccgggacg tgtacgacaa cctgttcgat ccggtaaacc aatacaccgt
     1561 gaccttcgac tcgcagggcg ggacggcggt atcggcccag acggtggatt
//
LOCUS       DAORHF010000458         1606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_103255_length_1606_cov_4.922631, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000458 DAORHF010000000
VERSION     DAORHF010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103255_length_1606_cov_4.922631"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PKJ16_19745"
     CDS             <1..710
                     /locus_tag="PKJ16_19745"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HNV49282.1"
                     /translation="IKRAKIDIAEISISRITEQYIAYLSLMEKNDTQVAAEFIVMASE
                     LLYYKSKSILPSDAIEDEYFVPPLPPDLIAKLLEFKKYQMASHELQSQFDQRADMFSR
                     PTVPAEFDDVDATLIEVSLFDLLAAFADVMAETVETPEMEIVFDEILVSERIVHIQNL
                     LGAREQVLFAELFSERPRRMEIVATFLAILELAKMHAVRLYQHAVFGDIHIVRGGWDP
                     GVAAAGDGAEGSIADGE"
     gene            710..1342
                     /gene="scpB"
                     /locus_tag="PKJ16_19750"
     CDS             710..1342
                     /gene="scpB"
                     /locus_tag="PKJ16_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HNV49283.1"
                     /translation="MAVNEEELEQGLEPSGEGAGDAEGGLPEEEDRDRLDLHGLFEAV
                     LFISSEPLPAGFFVKNFGVDATHARIILDSLVDAYEERDGGVKIVRLSNGYQYVTDRR
                     YAEQIRRVLGLSRKETLSKSMMETLAIVAYKQPIVISEIDELRGVSSRMMVANLLKKN
                     LIKPVGRKELPGRPLAYGTTDDFLKYFGLNKLSDLPRLSEIKEFTIQHGE"
     gene            1346..>1606
                     /locus_tag="PKJ16_19755"
     CDS             1346..>1606
                     /locus_tag="PKJ16_19755"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HNV49284.1"
                     /translation="MMGIMEDLDRRIIELINERVRRYVDEMKRRGEGAADAFSPMDRV
                     RVYEQIANYNGGPIASDALRKIYTEIISNALVQACPTRVAYLG"
BASE COUNT          323 a          455 c          520 g          308 t
ORIGIN      
        1 tcatcaagcg cgcgaagata gacatcgcgg agatttccat atcgcgcatc accgagcagt
       61 atatcgccta tctgagcctc atggagaaga acgacaccca ggtggccgcg gaattcatcg
      121 tcatggcctc ggagttgttg tactataagt cgaaatcgat ccttccctcc gacgccatcg
      181 aggacgagta cttcgttccc ccgcttccgc ccgatctcat cgccaagctg cttgagttca
      241 agaagtacca gatggcgtcg cacgagctgc agagccagtt cgaccagcgc gcggacatgt
      301 tctcgcggcc gacggttccc gccgagttcg acgacgtgga tgcgacgctg atcgaggtgt
      361 cgctcttcga tctgctggcg gcgttcgccg acgtgatggc cgagaccgtc gagacgcccg
      421 agatggaaat tgtatttgac gaaatcctgg tgagcgaaag gatcgtgcat atccaaaatt
      481 tgctgggagc gcgcgagcag gtgctgttcg cggagttgtt ttccgagcgg ccgcggcgca
      541 tggagattgt cgccaccttt ctggccattc tcgaactggc gaagatgcat gcggtgcgac
      601 tctatcagca tgcggtgttt ggcgatatcc acatcgtccg cggcggatgg gatccgggag
      661 ttgcggcggc cggtgacggc gccgaagggt cgattgcaga cggcgagtga tggcagtgaa
      721 cgaagaggaa ctcgaacagg ggctggagcc ttccggcgag ggcgcgggcg acgccgaggg
      781 cggacttccg gaggaggagg accgcgaccg gctggacctg cacggccttt ttgaggcggt
      841 gctgtttatt tcgagcgagc cgctgcctgc gggttttttt gtgaagaact tcggggtgga
      901 cgcgacgcac gcgcgcatca tcctcgattc gctggtcgat gcctacgagg agcgcgacgg
      961 cggcgtgaag atcgtccggc tctccaacgg ctatcaatat gtgaccgacc ggcggtacgc
     1021 cgagcagatt cggcgggtgc tgggcctctc ccgcaaggag accctctcga agagcatgat
     1081 ggaaacgctg gcgatcgtcg cctataagca gcccatcgtc atctcggaga tcgacgagct
     1141 tcgcggcgtg tcgtcgcgca tgatggtcgc caaccttctc aagaagaacc tcatcaaacc
     1201 cgtggggaga aaggagctgc cggggcggcc gctcgcctac ggcaccacgg acgattttct
     1261 caagtacttc gggctcaaca aactctccga cctgccgcgg ctttctgaaa taaaggaatt
     1321 taccatacag cacggtgaat aggcgatgat gggaatcatg gaggacctgg accggcggat
     1381 catcgaactc atcaacgaac gcgtgcggcg gtatgtcgat gagatgaagc ggcgtggcga
     1441 gggcgccgcg gacgccttct cgcccatgga ccgtgtccgg gtgtacgagc agatcgcgaa
     1501 ctacaacggc ggcccgatcg catcggacgc gctgcgcaag atatacaccg agatcatctc
     1561 gaatgcgctc gtgcaggcgt gtcccacccg cgtggcctat ctgggg
//
LOCUS       DAORHF010000459         1597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_104190_length_1597_cov_3.251621, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000459 DAORHF010000000
VERSION     DAORHF010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104190_length_1597_cov_3.251621"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PKJ16_19760"
     CDS             <1..479
                     /locus_tag="PKJ16_19760"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HNV49285.1"
                     /translation="WALTGCKGRAPQLLKSLYLADGEQEAHNLVMKQKYDIIRDREVM
                     YETRHLEDAELMIVAFGTPARIAKTAIENLRARGIRAGLLRLISLFPFPVHQVHDFSI
                     RIKKILVVEMNTGQMLRDVQISAHRDSRIFFYGRPGGGVPYPDDIEAQVVAALEEA"
     gene            483..1235
                     /locus_tag="PKJ16_19765"
     CDS             483..1235
                     /locus_tag="PKJ16_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HNV49286.1"
                     /translation="MNRIFQRPASLLDRPTHFCPGCTHGIAHRLIAEAIDELGIRERT
                     IGIPGVGCCVFLYHYFPIDVLEAPHGRAPACATGMKRAQPDKIIFAYQGDGDMAAIGT
                     SEVIHAANRGENVTVFYINNSVYGMTGGQMAPTTLLGQQTTTSPYGRDFKSEGYPIRM
                     AEMLATLEGVCYSTRVALHTPRHVNQARKAVIKALEMQNAEMGFSVVEMLSTCNTNWR
                     VTPLEAIDMIENEMIPYYPLGVFKERTGKDFL"
     gene            1263..>1597
                     /locus_tag="PKJ16_19770"
     CDS             1263..>1597
                     /locus_tag="PKJ16_19770"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HNV49287.1"
                     /translation="MGGFGGQGIQVISQLVVEAAIRKGLEVTYLPSYGVEKRGGRTTV
                     TVVLSDHEIGSPITNHPLCVIAMDVLALDKYQAMVAPGGSLIANTSLIPDEKIERGDI
                     SAITIRCNE"
BASE COUNT          351 a          467 c          477 g          302 t
ORIGIN      
        1 cctgggcgct caccggatgc aaagggcgcg cgccgcaatt gctcaagtcc ctctatctcg
       61 cagacggaga gcaggaagcg cacaacctgg tcatgaaaca gaaatacgat atcatccgcg
      121 atcgcgaggt catgtacgaa acgcgacacc tcgaggacgc cgaactgatg attgtcgcat
      181 tcggcacgcc ggctcgtatc gcgaaaaccg ccattgagaa ccttcgcgca cgcggcatca
      241 gggcgggtct gttgcgcctc atcagcctct ttccttttcc agtgcatcag gtgcatgatt
      301 tttctattcg aatcaagaaa atcctggtgg tcgagatgaa caccgggcaa atgctccgcg
      361 acgtgcagat ttccgcgcat cgtgattcgc ggatattttt ttacggccgg ccgggaggcg
      421 gggtccccta tcccgacgac attgaggcgc aagtcgttgc agcgctcgag gaggcgtagt
      481 cggtgaatag aatattccag cggcccgcat cgctcctgga caggccgacc cacttctgtc
      541 ccggatgtac ccacggcatc gcacaccggc tcatcgccga agcgatagac gaactgggaa
      601 tacgcgaacg caccatcggc ataccgggcg tcggctgctg cgtgttcctt taccattact
      661 tccccattga cgtacttgag gcgccgcatg gaagggcccc ggcatgcgcg accggcatga
      721 agcgcgcaca gccggacaag ataatattcg cctatcaggg tgatggcgac atggccgcca
      781 tcggcacctc ggaggtcatt cacgccgcaa accgcggaga aaatgtcacc gtcttttaca
      841 tcaataattc ggtatacggg atgacgggcg gccagatggc gccgacgacg cttcttggac
      901 agcagacgac caccagcccc tacggtcgag acttcaagag cgagggatat ccgattcgca
      961 tggccgagat gctcgcgaca ttggagggcg tctgttactc gacccgcgtt gcgcttcaca
     1021 cgccgcggca tgtcaaccag gcgcgcaagg cggtcatcaa ggcgctcgag atgcagaacg
     1081 cggagatggg cttttcggtg gtggagatgc tctcgacctg caacacgaac tggcgcgtaa
     1141 caccgctcga ggcgatcgac atgatcgaga atgaaatgat accctattat ccgctcgggg
     1201 tgttcaagga acggaccggg aaggacttcc tgtgaggtat gcgcatgtac ggtgatgtga
     1261 tcatgggcgg gttcggcggt cagggcattc aggtcatcag ccagctcgtc gtggaggcgg
     1321 cgatccgcaa agggctcgag gtgacctatc tgccgtccta cggcgtggaa aagcgcggcg
     1381 ggcgcaccac ggtgaccgtc gtgctgtcgg accacgagat cggatcgccc atcaccaatc
     1441 acccgctctg cgtgatcgcc atggacgtgc tcgcgctcga taaatatcag gcaatggtcg
     1501 ccccgggagg atcactgatc gcgaatacca gccttatccc ggatgagaaa atcgaacgtg
     1561 gcgatatctc cgccattacg atccggtgca acgaaga
//
LOCUS       DAORHF010000460         1575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_106739_length_1575_cov_3.346711, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000460 DAORHF010000000
VERSION     DAORHF010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106739_length_1575_cov_3.346711"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PKJ16_19775"
     CDS             complement(<1..292)
                     /locus_tag="PKJ16_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49288.1"
                     /translation="MITTRQHRTASAVIALALGLFAILRPAPLCAEDAPERFPVLLGV
                     GVLYGATGMIDYDTPPERNQEWIGDRMWGGGLVFEKMFKDFGGELPLPTQMLI"
     gene            complement(315..680)
                     /locus_tag="PKJ16_19780"
     CDS             complement(315..680)
                     /locus_tag="PKJ16_19780"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HNV49289.1"
                     /translation="MADSKEMVEDENKIGTVIGDDIEFRGTLSFKKSLKIKGFFEGKI
                     ETDGHLLVGQDARVSADIRASVVSVNGRVNGTIKADKKVELFSDSVTMADIIAPDLSI
                     ESGSSFNGVCVMNGAPAKE"
     gene            816..1205
                     /locus_tag="PKJ16_19785"
     CDS             816..1205
                     /locus_tag="PKJ16_19785"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HNV49290.1"
                     /translation="MEELLAKANELGLMIKGTELYKRLVDISGKIDRDEPAKALLEEY
                     ISASEELHYKEQTGQPIEAEEKKKIKELSERAAQSDLIKEYIATQSYYMNLMMQIQKA
                     ISEPVGEPIAESRIIKPTEGGKIITDF"
     gene            complement(1206..>1575)
                     /locus_tag="PKJ16_19790"
     CDS             complement(1206..>1575)
                     /locus_tag="PKJ16_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49291.1"
                     /translation="GKAADNHVVPIFRDSLLAKLQAGGFTINDKNAGLSIRQTIQVTS
                     QSQALKVFNPNMNSIRVGGHLSFTMAAGTVLGSWNNSEAGVHIDTKTAAGTAAQRIAD
                     KAARYIIRTILEKYAAGQLP"
BASE COUNT          342 a          451 c          462 g          320 t
ORIGIN      
        1 cgatcagcat ctgggtcggg agcggcaact cgccaccgaa gtccttgaac atcttctcga
       61 agaccagacc gccgccccac atgcgatcgc cgatccattc ctggttccgc tcgggcgggg
      121 tatcgtaatc gatcatcccg gtcgcgccat acaggacccc gacgcccagc agcaccggga
      181 atcgttcagg cgcgtcctcg gcgcacagcg gggcggggcg gaggatggcg aaaagcccca
      241 gcgcaagcgc gatcacggcc gacgcggttc gatgctgtcg cgtggtgatc atgcgaatcg
      301 gttccgatac tggatcattc cttggccggt gcgccgttca tgacgcacac gccgttgaag
      361 ctggaaccgc tttcgatgga gaggtccggc gcgatgatgt cggccatcgt gacgctgtcg
      421 ctgaagagct cgactttttt gtcggccttg atggtgccgt taacgcgtcc gttcaccgag
      481 acgacgctcg cgcgaatgtc ggcgctcacg cgggcgtcct gacctacgag aaggtgtccg
      541 tcggtctcga tcttgccctc gaaaaagcct ttgatcttga gtgatttctt gaaggagagc
      601 gttcccctga attcaatgtc gtcgccgatg acggtcccga tcttgttttc gtcctcgacc
      661 atctctttgg aatctgccat gcgtcctcct gcgggattcg tgcgttgatt gcgtatacac
      721 tgtacaaagg cgcgctctaa tttcaagaaa ataatttaca aatgatgatt attgaaaacg
      781 aatggccgtc actgctgatt cagggggcgt gtacgatgga agagctgctg gcgaaggcga
      841 acgagcttgg actcatgata aaagggacgg agctctacaa gcgcttggtc gatatttccg
      901 ggaagatcga tcgcgacgag cccgcgaagg cgctcctgga ggagtacata tccgcgagcg
      961 aagagctgca ctacaaggag cagacgggcc agcccatcga ggcggaggaa aaaaagaaga
     1021 tcaaagagct ctccgaacgg gcggcccaga gcgacctcat caaagagtat atcgcgaccc
     1081 agtcgtacta catgaacctg atgatgcaga tacaaaaagc catcagcgag cccgtgggcg
     1141 agccgatagc cgaatcgcgc atcatcaagc ccaccgaggg cgggaaaatc atcaccgact
     1201 tttaatcacg ggagctggcc ggcggcgtat ttctccagta tcgttcgtat gatgtagcgc
     1261 gccgccttgt cggcgatccg ctgggccgcg gtcccggccg ccgttttcgt atcgatatgc
     1321 acgcccgcct ctgaattgtt ccaggacccc agcaccgtcc ccgcggccat cgtaaaactc
     1381 agatgcccgc ccacccgaat ggaattcata ttgggattaa aaaccttaag cgcctgcgac
     1441 tgcgaggtga cctgaatggt ctggcgaatg gagaggccgg cgttcttgtc gttgatggta
     1501 aaaccgcctg cctgcaattt cgccaacagg ctgtcgcgga agatgggaac gacatgatta
     1561 tccgccgcct tgccg
//
LOCUS       DAORHF010000461         1571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_107125_length_1571_cov_5.527704, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000461 DAORHF010000000
VERSION     DAORHF010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107125_length_1571_cov_5.527704"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="PKJ16_19795"
     CDS             complement(<1..290)
                     /locus_tag="PKJ16_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49292.1"
                     /translation="MSDTLSKYKWRAIIGGTIAVISYMYFFAGSKQDPVERKVQELSY
                     YKPRFDDSISLEKKLLLMGLIDEQRHSSNEINDYLLFVYEEHGKDIHRPWGQ"
     gene            complement(338..1396)
                     /locus_tag="PKJ16_19800"
     CDS             complement(338..1396)
                     /locus_tag="PKJ16_19800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HNV49293.1"
                     /translation="MKRIIRPTSIIVISLFLVATPFINLYQLLFFGQRTHWIVTQHPF
                     TPASILGFTMIVLALCAGIGIYQIRRWGWYCFLAFAVVILAVSAYRIVTVRTAFAILI
                     AALNVAVVMGTLYLISRNVRKPYFSNEFRGWRIARRVPLRLPVQLLLNPLTGRSLDAT
                     TEDFSELGALIPLANNEPGFSLGQTVRMSIKLENDEPMTVAGEIVQFRDGSDRAACIG
                     LRFYVDDARRNRIARFIRHRYTPRYECAFPIDVATASGNALRGTVSNMSSGGCYVMCS
                     SGLPLAGEEVECTLYTKERARERATIGFSGTITWVNRDGRYDKPRGFGVHIGRVSMGR
                     VAFILLHLFNLRRKALVR"
BASE COUNT          353 a          442 c          452 g          324 t
ORIGIN      
        1 tattggcccc agggccggtg aatgtccttc ccgtgctcct cgtagacgaa gaggagataa
       61 tcgtttattt catttgatga atggcgctgc tcatcgataa gccccatgag cagcagtttt
      121 ttttcgagcg aaatcgagtc gtcgaaacgc ggtttgtaat aggagagctc ctggaccttg
      181 cgttcgaccg ggtcctgctt ggagccggcg aaaaagtaca tgtatgatat gacggcgatg
      241 gtgccgccga tgatcgcgcg ccatttatat ttcgagagtg tgtcactcat acgtgctccc
      301 gataggttcg acccggctat tcatgacggt attcacgtta tctcaccagg gcctttcggc
      361 gcaagttgaa cagatgcaga aggatgaagg ccacccgccc cattgacacg cgcccgatat
      421 ggacgccgaa tcccctcggt ttgtcatatc gtccatcacg attgacccag gtgatcgtcc
      481 cggaaaaacc gattgtcgcg cgttccctcg cccgctcctt cgtatacagg gtgcattcga
      541 cctcctcccc ggccaacgga aggccgctgg aacacatcac ataacagccg ccggacgaca
      601 tattgctcac cgtgccgcga agcgcgtttc cggaggcagt ggccacatcg atgggaaacg
      661 cgcactcgta tctcggcgta tatcgatgcc gtataaatcg cgcgatacgg ttgcggcgcg
      721 catcatcgac ataaaaccgg agaccgatac aggcggcgcg atcggagccg tctcgaaact
      781 gtacgatctc gccggcgacg gtcatcggtt cgtcattttc gagcttgatc gacatccgca
      841 cggtctgtcc cagtgaaaat cccggttcat tatttgcaag tggaattaat gcgcccaatt
      901 ccgaaaaatc ttccgtcgtc gcgtccaggg agcggcccgt gagcggattg agcagcaatt
      961 gcacgggaag ccgcagggga acgcgtcgag cgatgcgcca cccgcggaac tcgttcgaaa
     1021 aatacggttt gcgcacattt cgcgagatga ggtacagcgt tcccatcacc acggccacat
     1081 tgagggcggc aatcaggatg gcgaacgcgg tgcggacggt cacgatgcga taggcgctca
     1141 cggcgaggat gacgaccgcg aaggcgagaa agcagtacca gccccagcgg cgtatctgat
     1201 agatgccgat ccccgcgcag agggccagga caatcatggt aaagccgaga atggaggccg
     1261 gcgtaaacgg atgttgcgtc acaatccagt gcgtccgctg tccgaagaag agcagttggt
     1321 acagattaat gaacggtgtt gcgaccagga acagcgaaat cacgataatg gacgtcggtc
     1381 gaatgatccg tttcatgtgc tctccttcac tgatgcgcgg tgtctcatgg cgcgtcgcag
     1441 aacgtcatga cgtataatac catatcgccc tcgatcgtcc ggtcgacgga gtggccgcgc
     1501 ttccgggcat ggcgcacgag cccgatcatc gccttgttct ccgtgagcac atcgacggtg
     1561 aagcccgata t
//
LOCUS       DAORHF010000462         1545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_110141_length_1545_cov_4.640268, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000462 DAORHF010000000
VERSION     DAORHF010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110141_length_1545_cov_4.640268"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PKJ16_19805"
     CDS             complement(<1..550)
                     /locus_tag="PKJ16_19805"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HNV49294.1"
                     /translation="MKSATTPEFHIPTQVFLKEDILAEAGEICTLFGSRAVLATTSAD
                     FNQFQDVIENIAQTLKNSNVSCIIYDEMPTSLDTEYLDSASSFLKKTQCDLIIGFGGL
                     ESINAAKAAAVLVNNFIFADELHSTENLKPPVNLVTMPAFPLFGFEISPMLYLNDIHD
                     NVKKLFKRRDLFPVGTIIDPSIA"
     gene            complement(648..944)
                     /gene="flgM"
                     /locus_tag="PKJ16_19810"
     CDS             complement(648..944)
                     /gene="flgM"
                     /locus_tag="PKJ16_19810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HNV49295.1"
                     /translation="MVIDKIGNINNIIETKSAKTVSKAKSPKKSDMVEISSEGKKAQE
                     TAQLGQIIRETPDIRREKVEAIKAQIKDGTYDKFVDNAVLEMVADKIASNLLRR"
     gene            complement(1164..>1545)
                     /locus_tag="PKJ16_19815"
     CDS             complement(1164..>1545)
                     /locus_tag="PKJ16_19815"
                     /inference="COORDINATES: protein motif:HMM:NF012511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HNV49296.1"
                     /translation="YGDLEALSAENGLAAIQFFTPTSTDERMMRLAQGARGFVYCVAR
                     RGVTGSATELDRGLYDLLARYRRMTDLPLALGFGIASADDIRRLRGVVDIAIVGSALL
                     RSWERGGEDAYRAHLTEMAQARYS"
BASE COUNT          285 a          430 c          452 g          378 t
ORIGIN      
        1 tcgcgatgga cggatcgatg atggtgccca ccggaaacag gtcgcggcgc ttgaagagct
       61 tcttcacgtt gtcgtggatg tcgttcaggt acagcatggg cgagatctcg aagccgaaca
      121 ggggaaaggc cggcatggtg acgaggttca ccggcggctt caggttctcg gtcgagtgca
      181 gctcgtcggc gaaaatgaaa ttgttcacca gcacggcggc cgccttcgcc gcgttgatgg
      241 actccagccc cccgaagccg atgatgagat cgcattgcgt cttcttaagg aacgaggagg
      301 cgctgtcgag atactcggta tcgagcgagg tgggcatctc gtcgtagatg atgcatgaca
      361 cgttcgagtt cttgagggtc tgggcgatgt tttcgatgac gtcctggaac tggttgaaat
      421 ctgcggaggt ggtcgccagc acggcgcgcg aaccgaacag cgtgcagatt tcgcccgcct
      481 cggccaggat gtcctccttg aggaacacct gtgtcggaat gtggaattcc ggggtagttg
      541 cgcttttcac atccgtttcc cgtagtggag ataaaaaaag cggaactcag ggttgaagtc
      601 cgctttcctc atgccgcgtc cgtacgcgca ccgcgccgga tcgtttgcta acggcgtaaa
      661 aggttcgatg cgatcttgtc ggcgaccatc tccagcaccg cattgtcgac gaatttatcg
      721 taggtgccgt ccttgatttg cgcctttatg gcctcgacct tctcgcggcg gatgtccggc
      781 gtttccctga tgatctggcc gagctgtgcc gtttcctggg ccttcttccc ctcggaagag
      841 atttcgacca tgtcgctctt cttgggggat ttcgccttgc ttaccgtctt ggcgctcttg
      901 gtctcgataa tgttgttgat gttcccgatt ttgtcaataa ccatcgtttg taaccctttt
      961 attatctgtt cctattatcg gtcgttttcg acatttcttt actttatttc cagttgaata
     1021 ttcgcttttc gtttgtattt ttactcaaaa tacaaaagtg taaagatgta tgatgacgcc
     1081 ttccggcgcg tcctgacttc gttacgaacg taacatactc acaaatcgac agaatgtcaa
     1141 gcaactgtcc ttggaacgcc ccgttagctg tatctggcct gcgccatttc ggtaagatgc
     1201 gcccgatagg cgtcctctcc gccacgctcc catgaccgaa gcagggccga tccgacgatg
     1261 gcgatatcga cgacaccccg gagccgcctg atgtcgtccg cgctcgcgat gccgaagccc
     1321 agggcgagcg gcagatcggt catgcggcgg tagcgggcga gcaggtcgta caggccccgg
     1381 tccagctcgg tcgcgcttcc cgtgacgccg cggcgggcga cgcagtagac gaacccccgc
     1441 gcgccctgcg cgaggcgcat catgcgctcg tcggtgctgg ttggcgtgaa gaattgaatt
     1501 gccgcaaggc cgttctccgc cgaaagggcc tccaggtcgc cgtat
//
LOCUS       DAORHF010000463         1537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_111139_length_1537_cov_4.692308, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000463 DAORHF010000000
VERSION     DAORHF010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111139_length_1537_cov_4.692308"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1537)
                     /gene="dnaE"
                     /locus_tag="PKJ16_19820"
     CDS             complement(<1..>1537)
                     /gene="dnaE"
                     /locus_tag="PKJ16_19820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HNV49297.1"
                     /translation="DYSILDGAITVDRLISQAAAFGMPGVAMTDHGNMFGAIEFYQKA
                     RAKGIKPILGQEFYVAPRSRFEREPRSDGKDTSHHLLLIARDLEGYKNLIKLSSYGYT
                     EGFYFKPRIDRELLEAHSRGLICASACLGGIIPSYILAGKTDEAANIAGFYNELFGPD
                     SFYLELQDHGIKEQAIVNRELVALSARLGIPLIATNDCHFPRKEDSFSHEILLCIQTG
                     KTLENEQRMRFSSDQFYFKSSDEMYALFADYPDALYNTYKIFEMVDVTLNLGDAILPH
                     FAVPEGFTLNTYLEHLVAEGARKRFGEPVPATVLERIEYELSVITRMNFSGYFLVVWD
                     FINYAKSQKIPVGPGRGSAAGSMVSYCLGITELDPIRYNLLFERFLNPDRNEMPDMDI
                     DFCANRREEIIAYVKQKYGETHVSQIITFNKMKAKAVVKDVARVLNIPFTESNEITKL
                     ITENTLEEVMKTSKEFKEIAKSGKKGKTLVDTALNLEGLVRSAGKHAAGIVISREPLV
                     EYVP"
BASE COUNT          301 a          407 c          485 g          344 t
ORIGIN      
        1 gggacgtatt cgacaagcgg ttcgcgggag atgacgatgc cggcggcgtg cttgcccgca
       61 gaacggacaa gcccctcgag gttgagagcg gtatcgacga gggtttttcc ttttttaccg
      121 cttttcgcaa tctccttgaa ttcctttgag gtcttcatga cctcttccag cgtgttttcg
      181 gtaatgagct tggtgatctc gttggattcc gtgaacggaa tgttgagcac gcgggcgacg
      241 tccttgacga cggccttcgc cttcatttta ttaaaggtga tgatttggct cacatgcgtc
      301 tcgccgtact tctgcttcac gtacgcgatg atctcctcgc ggcgattggc gcagaagtcg
      361 atgtccatgt caggcatctc gtttctgtcg gggttgagga agcgctcgaa gagcagattg
      421 taccggatcg ggtcgagctc ggtgatcccg aggcagtacg acaccatcga tccggcggcg
      481 gacccgcgtc cgggtcccac ggggatcttc tggctcttgg cgtagttgat gaaatcccat
      541 acgacaagga aataaccgga gaaattcatg cgggtgatga cggacagctc gtactcgatg
      601 cgctcaagga ccgtcgcggg gaccggttcg ccgaatcgtt tccgcgcgcc ctcggcgacc
      661 agatgctcga ggtaggtgtt gagcgtaaat ccctcgggaa cggcgaaatg cggaaggatg
      721 gcgtcgccga ggttgagcgt gacatcgacc atctcgaaga tcttgtaagt gttgtagagg
      781 gcgtccgggt aatcggcgaa aagcgcatac atctcgtcgg acgacttgaa gtagaactgg
      841 tccgacgaga atcgcattcg ttgctcgttt tcgagggtct tgccggtttg aatgcacagg
      901 agaatctcgt gggaaaatga gtcctctttc cgggggaagt gacagtcgtt cgtcgcgatg
      961 agcggtatac cgagccgggc ggagagggcg acaagctcgc gattcacgat cgcctgctcc
     1021 ttgatgccgt ggtcctggag ctcaagataa aaggaatcgg gcccgaagag ctcgttgtag
     1081 aacccggcga tgttcgccgc ctcatccgtc tttcccgcta ggatatacga ggggatgatc
     1141 ccgccaaggc acgccgaggc gcagatcagc ccgcgcgaat gcgcttcgag cagttcgcgg
     1201 tcgatgcgcg gcttgaaata aaacccctcg gtgtagccat acgatgagag tttgataagg
     1261 ttcttgtagc cctcgaggtc gcgcgcgatc aggagcaggt ggtgcgacgt gtcttttccg
     1321 tccgaacggg gctcccgctc gaatcgcgac cggggcgcga cgtaaaactc ctgtcccagg
     1381 attggcttaa tgcctttcgc gcgcgccttt tgatagaact cgatcgcgcc gaacatgttg
     1441 ccgtgatccg tcatcgcgac gccgggcatg ccgaaggccg ctgcctgcga tatgagccgg
     1501 tccacggtaa tggcgccgtc gaggatcgaa tagtcgg
//
LOCUS       DAORHF010000464         1529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_112115_length_1529_cov_4.883989, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000464 DAORHF010000000
VERSION     DAORHF010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112115_length_1529_cov_4.883989"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="PKJ16_19825"
     CDS             <1..621
                     /locus_tag="PKJ16_19825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HNV49298.1"
                     /translation="PDTLPQVFDPFFTTKEVGKGTGLGLSVVYGIIKQHKGWIHVESD
                     PGKGTAFSIFIPAVSSAVAAEPAGEVPPTPQGRDEGVLVVEDQNEVRHMAVKMLRLGG
                     YRVFEAETASEALAVFERERDRIGAIFCDVVLPDMSGPLLVERLCEMSPNCRVLFSSG
                     YSDERSQWDIIRGRGYNFIQKPYSLDELLKAVRKTIDGERDTAPAR"
     gene            complement(655..852)
                     /locus_tag="PKJ16_19830"
     CDS             complement(655..852)
                     /locus_tag="PKJ16_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HNV49299.1"
                     /translation="MSCKRARESEQNSCTGQCKEQFELAVQNCGAQGNEQAKANCQFA
                     AMKDQSACVQKCNEAFMKSVK"
     gene            complement(1098..>1529)
                     /locus_tag="PKJ16_19835"
     CDS             complement(1098..>1529)
                     /locus_tag="PKJ16_19835"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HNV49300.1"
                     /translation="ILLNESITATDHTEGMVRQERTMKREQLRTARKNQSMGQVAPAN
                     YNWAEREASLERSENALDAAEHARWQIRDRVAWAQVHINLRGPVAPDYIDVPPYRNAF
                     VGLANLLLRLVYVTIYLLPFIAIIAAIVWKRDSIRAIFKRT"
BASE COUNT          264 a          462 c          492 g          311 t
ORIGIN      
        1 cccgacacgc ttccgcaggt gttcgatccc ttcttcacca ccaaggaggt gggcaagggg
       61 accggcctgg ggctttcggt ggtgtacggc atcatcaagc agcacaaggg gtggatccac
      121 gtggagagcg atcccgggaa ggggaccgcc ttcagcattt tcattccagc ggtgtcgagc
      181 gcggtggcgg ctgagccggc cggcgaggtg cccccgacgc cgcaggggcg ggacgagggc
      241 gtcctggtgg tcgaggacca gaacgaggtc cgccacatgg ccgtgaagat gctgcggctc
      301 ggcgggtatc gggtcttcga ggccgagacg gcgtccgagg cgctcgcggt gttcgagcgg
      361 gagcgcgacc gcatcggcgc catcttctgc gacgtggtgc ttcccgacat gagcgggccg
      421 ctcctcgtgg agcgtctctg cgagatgtcc ccgaactgcc gcgtgctctt cagcagcggc
      481 tattccgatg aacgctcgca gtgggacatc atccgggggc gcgggtacaa cttcatccag
      541 aaaccctatt cgctcgacga gctcctcaag gcggtgcgaa aaacgataga cggcgagcgg
      601 gatacggcgc cggcgcggtg agtcgatcct gtccggacag tttcgcgata agccctattt
      661 caccgatttc atgaaagcct cgttgcattt ctgcacacag gcggattggt ctttcatcgc
      721 cgcgaactgg cagttcgcct tcgcctgttc attcccctgc gcgccgcagt tttgcacggc
      781 cagctcgaac tgctccttgc attgtccggt gcaggagttc tgttccgatt ctctcgccct
      841 tttgcatgac atggaaaaga tcgatacgca aaccgccgca agtgtgcacg cgataattgc
      901 ttgtctcatg gtccctccgt tatgccggaa tatgacatca tataatcgta ttaaaaaaca
      961 cctatggtcg atctgtcaat gacaatcgaa acgtcacgag cgctcgtttc gcgcagtgaa
     1021 aagatggggg gatcgcgatg gggcgcttcg gtcatcaccc ccatcgtttg gaggcgatgc
     1081 ccgaagcgcg gctccgtcta cgttcgcttg aaaatcgccc tgatgctgtc gcgcttccag
     1141 acgatcgccg cgatgatcgc gatgaacggc agcaggtaga tcgtcacata gaccaggcgc
     1201 agcagcaggt tcgccagccc cacgaaggcg ttccggtacg ggggcacgtc gatgtagtcg
     1261 ggcgcgaccg ggccgcggag gttgatatgc acctgcgccc aggcgacgcg gtcgcggatc
     1321 tgccagcgcg catgctcggc ggcgtccagg gcgttttcgc tgcgctcgag cgacgcctcg
     1381 cgctcggccc agttgtaatt cgcgggggcc acctgtccca tgctctggtt cttgcgcgcc
     1441 gtgcgaagct gctcgcgttt catggtccgt tcctggcgca ccatgccctc ggtgtggtcg
     1501 gtcgccgtga tcgattcgtt caggaggat
//
LOCUS       DAORHF010000465         1515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_113821_length_1515_cov_4.960959, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000465 DAORHF010000000
VERSION     DAORHF010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113821_length_1515_cov_4.960959"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /gene="rmuC"
                     /locus_tag="PKJ16_19840"
     CDS             complement(<1..1017)
                     /gene="rmuC"
                     /locus_tag="PKJ16_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007935349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HNV49301.1"
                     /translation="MFLEIALICIAVLLIANLALSWKAARSNSDAALVQMRTSLEYFE
                     KLLKSTETAMREEIARYRAESAGSAKDAREELSRTFAGFSDAIARRIVEIAGQQKGQL
                     ELFSKQLADLMRLNEQKLDTMRDTIEVRVRSLQEENSRKLDQMRDTVDEKLQTTLEKR
                     LGESFKLVSERLEMVHKGLGEMQGLASDVGDLKKVLTNVKSRGVWGEIQLGNLLEQVL
                     TAEQYEKNVAIKERSQERVEFAIKLPGRGESARDVVWLPIDAKFPQEDYQRLVDAQER
                     ANPAMAEEAAKRLEGRIKLEAKAIREKYIDPPQTTDFAIMYLPTEGLYAEVIRRPGLA
                     DSLQR"
     gene            complement(1235..>1515)
                     /locus_tag="PKJ16_19845"
     CDS             complement(1235..>1515)
                     /locus_tag="PKJ16_19845"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587278.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the K subunit is a
                     nonenzymatic component which binds the dimeric form by
                     interacting with the G and E subunits; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HNV49302.1"
                     /translation="FVGAPLSQTIYGMILMNAIAAAQNTDPLIKLGVGVFGGLAIGAS
                     AFYQGKAGASGSDALAETGKGFGNYIMVVGIIETVALFVMVFLLGMLK"
BASE COUNT          277 a          516 c          426 g          296 t
ORIGIN      
        1 ccgctgcagg gaatcggcca ggcccgggcg acggatcacc tcggcgtaca acccctccgt
       61 gggaagatac atgatggcga aatccgtcgt ctgcggcgga tcgatgtact tctcgcgaat
      121 cgccttcgct tccagtttga tccgcccctc gaggcgcttc gccgcctcct cggccatggc
      181 cgggttcgcg cgctcctggg cgtcgacgag acgctgatag tcctcctgcg ggaatttcgc
      241 gtcgatgggc agccacacca cgtcccgcgc cgactccccc cgcccaggca gcttgatggc
      301 gaactccacg cgctcctggc ttcgctcctt gatggcgacg ttcttctcgt actgctcggc
      361 ggtgagcacc tgctcgagca ggttccccag ctggatctcg ccccataccc cgcgcgactt
      421 aacgttggtg agcacctttt tcagatcacc cacgtcggag gccagtccct gcatctcccc
      481 caaccccttg tgcaccatct cgaggcgctc gctgacgagt ttgaacgatt cgcccagccg
      541 cttttcgagc gtcgtttgca gtttctcgtc aaccgtatcg cgcatctggt cgagcttgcg
      601 gctgttctcc tcctgcagcg aacgcacgcg gacctcgatg gtgtcgcgca tcgtgtcgag
      661 cttctgctcg ttgagccgca tgaggtccgc gagctgcttc gaaaaaagct cgagctggcc
      721 cttctgctgt ccggcgatct cgacgatacg gcgcgcgatg gcgtcggaga acccggcgaa
      781 tgtccggctc agctcctcgc gcgcgtcctt cgccgatccg gcggactcgg cacggtagcg
      841 cgcgatctcc tcccgcatcg ccgtctcggt cgacttgagg agcttttcga agtattccaa
      901 tgatgtccgc atctgcacca atgcggcgtc gctgttcgaa cgcgcggcct tccaggagag
      961 cgccaggttg gcgatcagca gtaccgcaat gcatatgagc gcgatttcca agaacatatc
     1021 tcacctcgtt gaaaacggca tgtgggaagg aatgcggcga cggcccggca tcgcaaccgt
     1081 ttcgaaggag cgatcgccgc cctccgaagg gtttggcgcg ggcggggaac acgcccgcgg
     1141 atcatactcg ctaaaatgga aaaagccggg agcgcgcccg aaacggcacc acccccggcc
     1201 tcgatgcgaa ggaatacgct cgtacgtcgt gggactactt gagcataccc agcaagaaca
     1261 ccatcacgaa cagcgcaaca gtctcgataa taccgacaac catgatgtag ttaccgaacc
     1321 ccttgcccgt ttccgccagt gcgtcggaac cgcttgcgcc cgcctttccc tggtaaaagg
     1381 ccgatgcgcc aatcgccaat cccccgaaca cacccactcc cagctttatc aatggatccg
     1441 tattctgggc cgcggcgatc gcgttcatca ggatcatgcc gtaaatcgtc tgcgacagcg
     1501 gagcgccgac aaacg
//
LOCUS       DAORHF010000466         1511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_114312_length_1511_cov_5.225275, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000466 DAORHF010000000
VERSION     DAORHF010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114312_length_1511_cov_5.225275"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1511
                     /locus_tag="PKJ16_19850"
     CDS             <1..>1511
                     /locus_tag="PKJ16_19850"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HNV49303.1"
                     /translation="AIRLHPLVCHPYNADFDGDQMAVHVPLSPKAQIECWTLMLSANN
                     LLSPANGHPVVTPTQDIVLGIFYLTSELHDSPGEGMMFDDEAEIHRAVDAGQIKIRTK
                     IKFIQGDTILETTAGRLIFNGILPDGFPYVNEVVNDSRLSKLISSVFRTYGPDTTVKF
                     LDDVKETGYRYATIFGPTISMSDIIVPEIKHQIIAASDKKVLAIEDEYRNGFITNDER
                     YNRVVNVWTQTNEEVAEKMFDEYKGATNGFNAIFVMAQSGARGSKQQIRQLSGMRGLM
                     AKPSGEIIDVPIRANFKEGLNVLEYFISTNGARKGLADTALKTADAGYLTRRLVDIAQ
                     DVVVTIEDCGTTVGLETTAIKEGDEIIESLGARALGRTLLYDLIHPISGEVICKANDI
                     ITEEVADIIDELKIDSIEIRSALTCESRHGICVRCYGRNLATARPVDIGEAVGIIAAQ
                     SIGQPGTQLTMRTFHIGGIASRSVEESEIKLNYPIFLKEMTFKTIKTDDKKTI"
BASE COUNT          331 a          481 c          446 g          253 t
ORIGIN      
        1 aggcgatccg gctgcacccg ctggtgtgcc atccatacaa cgccgacttc gacggcgacc
       61 agatggccgt ccacgtcccg ctctcgccca aggcgcagat cgagtgctgg acgctcatgc
      121 tttcggccaa taacctgctc tcgcccgcaa acggacaccc ggtcgtcacg cccacgcagg
      181 acatcgtgct cggcatcttc tatctcacct ccgagctgca tgactccccg ggcgagggga
      241 tgatgttcga cgacgaggcc gagatccatc gcgcggtcga cgccggtcag atcaagatac
      301 ggacgaaaat caaattcata cagggcgata ccattctgga gacgacggca ggccgcctca
      361 tcttcaacgg catactcccc gacgggttcc cgtacgtgaa cgaggtggtc aacgattcgc
      421 ggctctcgaa gctcatttcg tcggtgttcc gcacctatgg ccccgacacc accgtgaagt
      481 ttctcgatga cgtgaaggag accgggtacc ggtacgcgac gatcttcggc cccacgatct
      541 ccatgtccga tatcatcgtc cccgagatca aacaccagat catcgcggcg tcggacaaaa
      601 aggtgctggc catcgaggac gagtaccgga acggcttcat caccaacgac gaacgctaca
      661 accgcgtggt gaacgtctgg acgcagacca acgaagaggt cgcggaaaag atgttcgacg
      721 aatacaaggg cgcgacgaac ggatttaacg cgatcttcgt catggcgcag tcgggcgcgc
      781 gggggtcgaa gcagcagatc cgccagctct ccggcatgcg cggtctcatg gccaagccga
      841 gcggcgagat catcgacgtg ccgatccgtg cgaacttcaa ggaagggctc aatgtccttg
      901 agtacttcat ctcgaccaac ggcgcccgga aggggttggc cgatacggcg ctcaagaccg
      961 ccgacgcggg gtatctcacc cggcgcctgg tggatatcgc gcaggatgtc gtggtgacca
     1021 tcgaggattg cggcaccacg gtggggctcg agacgaccgc catcaaagag ggcgacgaaa
     1081 tcatcgaatc gctcggcgcg cgcgccctgg ggcggaccct gctgtacgat ctcatccatc
     1141 ccatctccgg cgaggtgatc tgcaaggcca acgacatcat caccgaggag gtcgccgaca
     1201 tcatcgacga gctcaagatc gactcgatcg agatccgatc ggcgctgacc tgcgaatcgc
     1261 ggcacggcat ttgcgttcgc tgctacgggc gcaacctggc gacggcgcgc cccgtggata
     1321 tcggcgaggc cgtgggcatc atcgccgcgc aatcgatcgg ccagccgggg acgcagttga
     1381 ccatgcgcac cttccatatc ggcggcatcg cctcccggtc ggtggaggag agcgaaatca
     1441 agctgaacta tcccatcttc ctcaaggaga tgacctttaa gaccatcaag accgacgaca
     1501 agaaaaccat c
//
LOCUS       DAORHF010000467         1500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Spirochaetota bacterium isolate AMR_MDS_1211
            NODE_115791_length_1500_cov_2.903114, whole genome shotgun
            sequence.
ACCESSION   DAORHF010000467 DAORHF010000000
VERSION     DAORHF010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32854416
            Sequence Read Archive: SRR4236660
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Spirochaetota bacterium (wastewater metagenome)
  ORGANISM  Spirochaetota bacterium
            Bacteria; Spirochaetota.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 18:38:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,971
            CDSs (total)                      :: 3,933
            Genes (coding)                    :: 3,913
            CDSs (with protein)               :: 3,913
            Genes (RNA)                       :: 38
            rRNAs                             :: 1 (5S)
            complete rRNAs                    :: 1 (5S)
            tRNAs                             :: 34
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 20
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 20
            Pseudo Genes (frameshifted)       :: 5 of 20
            Pseudo Genes (incomplete)         :: 13 of 20
            Pseudo Genes (internal stop)      :: 4 of 20
            Pseudo Genes (multiple problems)  :: 2 of 20
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Spirochaetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115791_length_1500_cov_2.903114"
                     /isolate="AMR_MDS_1211"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2202144"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.84 N 87.68 W"
                     /collection_date="2014-12-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="PKJ16_19855"
     CDS             <1..1417
                     /locus_tag="PKJ16_19855"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HNV49304.1"
                     /translation="NTSDTVQTNIVVEAMMEEVTDSPAESETIVELKPGEQRTIAVPV
                     ALTRKVLAIVEDRTMSVRLKVIFSGENTKRYYVETGKALVHNKNALTWDRTHHLGSFI
                     QPKESTVSTLTRNIMSQYAAKREKGISINLQIAMQVFDALQALGVTYAADPDNKYAES
                     KSRNAVDYVQLPWETLKKRAGDCDDLTVLFASMLESVGIRTAIVTAPNHVFMMFDTGV
                     PRECAYEVSVDPAEYVVRNDSVWVPVEITAVGKGFMVAWKEGVKGIGGAESGIDVVGT
                     DQAWEKYPPADIGGGSGVPLPDNDILTRLYSRDIAVLKQELIDAPMKRLQDAVKGNPN
                     DYKSWNSLGILYGRNGTLYKSIECFMASYKLKDDYLPAYINLGNVFMLLNDYTKAIFY
                     YKKALQLNPQTVAARLNVARAYYEMRDYDSAYEEYRKVIERNPSYAQKYSYLDRKRTD
                     ASKERAAFGTGRADSNIWMAE"
BASE COUNT          363 a          400 c          451 g          286 t
ORIGIN      
        1 gaacacgagc gacacggtgc aaacgaacat tgtcgtcgag gccatgatgg aggaggtgac
       61 tgattcaccg gccgaatcag agaccattgt cgagttgaag ccgggagagc aaagaacgat
      121 cgcggttccg gtagcgctca cgcgaaaggt ccttgcgatc gtcgaggacc gcacgatgtc
      181 cgtgcgcctc aaggtcattt ttagcggcga aaacaccaag cggtattatg ttgaaaccgg
      241 gaaagcattg gttcacaaca aaaacgccct cacctgggac cggacccatc atctcggcag
      301 ttttatccag cccaaggagt cgacggtatc gaccctcacg cgcaacatca tgtcgcagta
      361 cgcggccaaa cgcgagaagg gcatatcgat caatctgcaa atcgcgatgc aggtgttcga
      421 cgccctccag gcgctgggag tcacctacgc ggccgatccg gacaacaagt acgccgaatc
      481 gaaatcgcga aacgccgtcg actatgtgca gctcccgtgg gagacgctga aaaaacgggc
      541 gggcgattgc gacgacctga ccgtgctctt tgcgtcgatg ctggaatccg ttggcatccg
      601 caccgcgatc gtgaccgctc cgaaccacgt gttcatgatg ttcgacaccg gcgttccgcg
      661 cgagtgcgca tacgaggtga gcgtcgatcc ggcggagtac gtggtacgca acgacagcgt
      721 ctgggtgccg gttgagataa ccgcggtggg caagggattc atggttgcct ggaaggaggg
      781 ggtgaaaggc atcggaggcg ccgagtcggg tatcgatgtc gtcggcaccg atcaggcgtg
      841 ggagaagtac ccgcccgcgg atatcggcgg cgggtccggc gttcccctcc ctgataatga
      901 tatccttaca cgcctttata gccgtgatat cgccgttctc aagcaggagc ttattgatgc
      961 gccgatgaag cgccttcagg acgccgtgaa gggaaacccc aacgattaca agtcctggaa
     1021 ctcgcttggc attctctacg ggcgaaacgg gacgctgtac aagtcgatcg agtgcttcat
     1081 ggcgtcctac aagctgaaag acgattatct ccccgcttat atcaatctgg ggaatgtttt
     1141 catgctgctc aacgattaca ccaaggcgat cttctactac aagaaagcgc tccagctcaa
     1201 cccgcagaca gtggcggcgc ggctgaatgt cgcgcgggca tattacgaga tgcgcgacta
     1261 cgacagtgcc tatgaagaat accggaaagt gattgaacgg aacccgtcat acgcccaaaa
     1321 atactcgtat ctggatcgga aaagaacgga cgcgtcgaaa gagcgcgccg ccttcggaac
     1381 aggtcgggcg gattcgaaca tctggatggc ggagtaagga caagtgaagg tgtgcgatat
     1441 gatgaaaacg ctgcggcact ggatcatgat gatgtctttg atcggtgcga tcgtctcatg
//
